LOCUS       DATDOZ010000001         4498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3403541, whole genome shotgun sequence.
ACCESSION   DATDOZ010000001 DATDOZ010000000
VERSION     DATDOZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403541"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1540)
                     /locus_tag="VI541_00005"
     CDS             complement(107..1540)
                     /locus_tag="VI541_00005"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLF69322.1"
                     /translation="MSPAVRSRNNQAIWLTLFKVITDVVSILGGFYIAAYIRFRTDFF
                     GVTEAAVNYRGIIAVAVPVWLTLFGMMGMYDKTHTKSITEELRRVFNGVNLGLVALIM
                     VSFFLAQRDISRGFVATAWIGCLVSVTVGRLIFRKILHIARSHGRLCLPMLVVGLNEE
                     ARAIARTLARNSWIGFKPVGFVAANGELDGTDITRVEGLPVYGSAPDIAGAVDMSRAS
                     AVLVASSAVPEDKLARLYRDLQDYDVDVRVSAGLMNIAASRVAVEPLDGVPVLALRKI
                     QLKGSQAAVKRLFDLVMSAAMIAVLSPILIVVALAIKLTSSGPVFYKQIRVGQYGREF
                     KMIKFRTMVRGAHDLVVDLRDRNDADGLLFKIKDDPRVTRVGRKLRRWYLDEVPQLFN
                     VLSGRMSLVGPRPPLPEEVARYDDWLRGRIKVKPGMTGLWQVNGRHELSFADYVRYDL
                     FYVQNWSVVSDLHILWRTVPAILKHAG"
     gene            complement(1537..2649)
                     /locus_tag="VI541_00010"
     CDS             complement(1537..2649)
                     /locus_tag="VI541_00010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF69323.1"
                     /translation="MHDFLNQKGGAERVVAGMARLYEDAPIFTSLYLPDQTFEEFRSR
                     DVRTSFLQAVPFKQALFEALLPLYPRAFRSFDLSGFDLVISSSTHWAHHVRPREAFHV
                     VYCHNPPRWLYQTEEYLQAGSPAPGWTHGLLRPLLSHLRRLDQDAARGPDLYIANSQV
                     VAERIRRVYGREAPVVNPPVDTAKFRGPQQGTSMPERRPEGGEKHYLVVGRLLPYKRI
                     DLAIEACNRRRTPLVIVGEGPARASLERLAGPTVRFAGKVGEEELVRLYRGAHALIQC
                     GAEDFGIAPLEANAAGVPVVAFRNGGALETVITDVTGIFFPEQRGDELELALDKVEAS
                     TWDRTTLQAHAERFDESSFMRQLSRVIGERMNGSSA"
     gene            complement(2661..3845)
                     /locus_tag="VI541_00015"
     CDS             complement(2661..3845)
                     /locus_tag="VI541_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF69324.1"
                     /translation="MDIEVLFVDHESRLSGGEQDLLDLVAALGHGHPRIKIHAALPAE
                     GPLSRALADQGCGVHLVAFGDQMRKTSRWELQRRPHLAILRLMDAMRTARALGRLMDE
                     IKPRVVHTNSMKSHFLALVPARFRGIPVIWHVRDILGEGWLRRVFRFVGGRGPAHILC
                     ISEATRRQFDGSRAHSRSRVVYNGIDLRRFDDVDRAGWRLRLGAPGDEVLVGIVGQIS
                     GWKGQDTFIEAAGVVSKQVPEARFVVVGECLFPENEGEYERRIHTRASELGLDPVLVW
                     AGWSDDPQSVMSAIDVVVHASHLPEPFGRVIVEGMAAGRPVIASSEGAGPEIVRDGEG
                     LVIPPGEPGLLVDALLPLVTSGDVRKRMGESARAGARRFDIALTADAVASVYAELLGR
                     AP"
     gene            complement(3897..4202)
                     /locus_tag="VI541_00020"
     CDS             complement(3897..4202)
                     /locus_tag="VI541_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF69325.1"
                     /translation="MKAVESHIDYNAMGRREQRRTRLIAQVAASAGLDPETDLGSGGP
                     MLRTGEVAVLFGVSERCIRNWANAGKLPSVRTLGRHRLFPAREVALVLREAMKGDQR"
BASE COUNT          901 a         1408 c         1373 g          816 t
ORIGIN      
        1 tgagctgatg atgcgcagta ccagccagct gcccgtcccc cagacgagag ccttcgagag
       61 aggcgatctc gttcgggggg cttcggtcat caggaccgaa gcatattcat cccgcgtgct
      121 tgagtatcgc tgggactgtg cgccagagga tgtgcaggtc cgagaccacc gaccagttct
      181 gaacatagaa gaggtcgtac cggacgtaat ctgcgaatga gagctcgtgg cgtccgttga
      241 cctgccatag accggtcata ccaggcttga ccttgatgcg accgcggagc cagtcgtcgt
      301 agcgagcgac ctcctcgggg agtgggggcc ttggtccgac aaggctcatc ctgccgctga
      361 ggacgttgaa gagctgcggg acctcgtcca gataccaccg acgaagcttg cggcccacac
      421 gggtgaccct gggatcgtcc tttatcttga agagcagtcc gtcggcgtca ttgcggtccc
      481 tcaggtcgac gaccaggtca tgcgcgccgc gaaccatcgt gcggaacttg atcatcttga
      541 actcacgacc gtactggcca acacggatct gcttgtagaa gaccggaccc gagctcgtta
      601 gcttgatggc cagtgcaacc acgatgagga tcggcgacaa caccgcgatc atcgctgcgg
      661 acatcacgag gtcgaagagt cgcttgacgg ctgcctgtga acccttcagc tggatcttgc
      721 ggagcgcaag aacggggacc ccatcgagcg gttcgacggc aaccctcgaa gcggcgatgt
      781 tcatgagacc agccgagacc cgtacatcaa cgtcatagtc ctgaaggtct cggtatagac
      841 gcgccaactt gtcttcgggt acagccgaac tcgcaaccag aaccgcgctg gctcgcgaca
      901 tgtccacggc ccccgcgatg tcgggagcgg agccgtatac cggcaatccc tcgacacgag
      961 tgatgtcggt tccatcgagt tcgccgttcg cagcgacgaa gcctacgggc ttgaagccga
     1021 tccaactgtt gcgtgcaagg gtacgggcga tcgcacgagc ctcttcattg agaccgacga
     1081 cgagcatcgg caggcagagt cgcccatgag agcgtgcgat gtgcaggatc ttgcggaaga
     1141 tgagtcgccc aacggtgacc gatacgagac acccgatcca cgcggtggcc acgaatcctc
     1201 tagagatgtc gcgctgcgca aggaagaacg aaaccatgat gagggcaacg agtccgaggt
     1261 tcactccgtt gaatacgcgc ctgagctcct cggtgatcga cttcgtgtgg gtcttgtcat
     1321 acatgcccat catcccgaag agggtcagcc agaccggtac cgcgacagcg atgatgccgc
     1381 gatagttgac tgctgcttcc gtcacgccga agaagtcggt gcggaaacgg atatacgccg
     1441 cgatgtagaa gcccccgagg atcgaaacga cgtcagtgat gaccttgaag agggtcagcc
     1501 agatagcctg gttgtttcgg ctgcgtaccg cgggactcac gcagagctcc cgttcattct
     1561 ttctccgatg acgcgcgaga gctggcgcat gaatgagctt tcgtcgaagc gctccgcatg
     1621 tgcctggaga gtggtcctgt cccaggtgct tgcctcgact ttatcgagag ccagttccaa
     1681 ctcgtccccc ctctgttccg ggaagaagat ccccgttaca tcagtgataa cggtctcgag
     1741 ggctcctccg ttacgaaatg ccacaacggg gacccctgcg gcgttggcct cgaggggggc
     1801 gatgccgaag tcctcggccc cgcactggat cagagcatgg gccccccggt acagccggac
     1861 caactcttcc tctccgacct ttccggcaaa acggacggtt gggcctgcca ggcgctcgag
     1921 cgacgcccgg gccgggccct ctcccacgat caccaggggt gtccgtcggc ggttgcatgc
     1981 ctcgatcgcc aggtctatcc gcttataagg aaggagtcgc cctacgacta gatagtgttt
     2041 ttcacctcct tccggccggc gttccggcat cgaggttccc tgctggggcc cccgaaactt
     2101 ggcggtatcg acaggagggt tcacgacagg agcctctcgc ccgtaaacac gccggatccg
     2161 ctcggctacg acctgtgagt ttgcgatgta caggtccggc cccctggccg catcctggtc
     2221 gagcctccgc agatgactca agagagggcg tagtaacccg tgggtccacc cgggggccgg
     2281 actgcccgcc tggagatact cctctgtctg atagagccat cgcggtgggt tgtggcagta
     2341 gacgacatgg aacgcttccc tcggccggac gtggtgggcc cagtgtgtcg aactcgagat
     2401 gacaaggtcg aacccgctca ggtcgaagga gcgaaaggcc cgcgggtata gcggcaggag
     2461 ggcctcgaat aacgcctgtt tgaagggaac ggcctgcagg aaactcgtcc gaacatcacg
     2521 agaccgaaac tcttcgaagg tctgatcagg aaggtagagg ctggtgaaga tgggtgcatc
     2581 ctcgtagagg cgtgccatgc cagccacgac gcgctcggcc ccccctttct ggttcaggaa
     2641 gtcatgcacg agcgcggttt tcagggggcc ctcccgagaa gttccgcgta gaccgaggca
     2701 accgcatctg ccgttagtgc gatgtcgaac ctgcgggcgc cagcgcgggc cgactcaccc
     2761 atcctcttgc gaacatcccc ggaggtaact aaaggaagaa gagcatcgac gaggagcccg
     2821 ggttcaccag gtgggataac gagcccctcg ccatctctca cgatctcggg gcctgcaccc
     2881 tcggaactag cgatgactgg ccgtcccgct gccatccctt caacgatgac gcgcccgaaa
     2941 ggttcgggaa ggtgcgatgc atggaccacc acgtcgatcg cactcatgac cgactgggga
     3001 tcgtcgctcc aacccgccca gaccaggacc ggatctagcc cgagttcgct cgccctggtg
     3061 tggatcctgc gctcgtactc cccttcgttc tcggggaaga ggcactcgcc cacaacgacg
     3121 aagcgagctt ctggcacctg ttttgagaca actccggccg cctcgatgaa ggtgtcctgc
     3181 cccttccagc cggagatctg acccacgatc ccgacgagca cttcatcccc gggggccccc
     3241 agacgcagac gccaacccgc cctgtccaca tcgtcgaagc gccgcaggtc gatcccgttg
     3301 taaacgacac ggctacgcga gtgagctcga gacccgtcga actggcgccg agtcgcttcg
     3361 gagatgcaga ggatgtgcgc gggcccccgg cctccgacga atctgaagac cctccgtagc
     3421 cagccctcac ccaggatgtc gcggacatgc cagatcacag ggatacctcg aaagcgcgcc
     3481 ggaaccagcg cgaggaagtg cgacttcatc gagttggtat ggacgaccct cggcttgatc
     3541 tcgtccatca aacggccaag agcccgagcg gttcgcatcg cgtccatgag tctgaggatg
     3601 gcgagatggg gtcggcgctg gagttcccag cgcgaggtct ttcgcatctg gtcgccaaac
     3661 gcaactaagt gaacgccgca cccttgatcg gccagggcac gcgagagcgg cccctccgcc
     3721 ggaagggccg catggatctt gatgcggggg tgaccatgcc caagggctgc cacaaggtcg
     3781 agcaggtcct gctcgcctcc ggagaggcga gactcatggt cgacgaagag cacctcgata
     3841 tccacacccc gcagactacg acgaggcgat accgccccgt cgtaatcgag tggttgctag
     3901 cgctggtcgc ccttcatcgc ttcacgaagg accagggcca cctcgcgggc cgggaagaga
     3961 cggtgccgac caagggtccg cacgctcggg agtttccctg cgttcgccca gtttcggatg
     4021 cagcgctcgc tgactccgaa gagaactgcg acctcgccgg tgcggagcat ggggcccccg
     4081 cttccgagat cggtctcagg gtcgagacca gcactcgctg ctacctgggc gatcaatctg
     4141 gtgcgacgct gctcacgcct gcccatggcg ttgtagtcga tgtgactctc tactgctttc
     4201 atgcctaccc cctctgggtc gttgtcctgg ttcgtttcag ggcctggctt ttgccgcttg
     4261 ttccctcaaa tcaaggtaga ccactgcggc accctgttcc atacgccgtc gggcccattc
     4321 gcctgcgact tcaggcgtat aacgagcaag cacataccga cctttgggtc aggactatca
     4381 gcggataaaa gcagaaaacc cgccccgtca ggcaggattc gggggggccg atggagaact
     4441 caaactagga ccgaatatct agtgagggag aggccgcatg cgcaagtttt cgaagtta
//
LOCUS       DATDOZ010000002         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2880344, whole genome shotgun sequence.
ACCESSION   DATDOZ010000002 DATDOZ010000000
VERSION     DATDOZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880344"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..828
                     /locus_tag="VI541_00025"
     CDS             187..828
                     /locus_tag="VI541_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF69326.1"
                     /translation="MSGELAYPSSVPSVTEADAEATAKETARVEETFELVTRAKTGDQ
                     TAFAALYRMHVQSVYRYLAYRLRDNSTAEDLTAEVFVRALRKIGDFEWRGIDFSAWLL
                     RIARNILLDHVKSSGTRLEVVGISPGDVKEGSSPGSDLEVLDRLDREDLHKALNKLRP
                     EHQEVLYLRFLQGLSALETAQAMGKSEGAVRVLQFRALKHLVKIMQQGQEDAG"
     gene            825..1892
                     /locus_tag="VI541_00030"
     CDS             825..1892
                     /locus_tag="VI541_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69327.1"
                     /translation="MKPAHTTKGFSRLGKGLALGTGARSIPIDAIIHNLATLSSPSLS
                     QLAVERIESKVLGSLLFDPPPPASNDITDDDLTRHISDDEISAFLAESVSPPMVPVTS
                     PEAKPVSRPLVRTRALGFGTAFAVLVCSALIVAASTAGPGTLLHPLRTKIEAVRVFVA
                     PSPLPKANVYMEIAQNRLDSLRAITSDQPEVMKQTLGQMSLATLHSLRLIESADDGAT
                     KFDALRRLDRLANAQGRTLIQLMRSWDPRIDDALSSSYRVATYVETWTHAILTGRSLS
                     FLSGTFRPPPGPITVTGHGLVPITHPADGRTGEGNRQSTTSGDTHGDTQGPEPETCDM
                     ASAMLCVSVPDTVTLPALPSL"
     gene            1952..2869
                     /locus_tag="VI541_00035"
     CDS             1952..2869
                     /locus_tag="VI541_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69328.1"
                     /translation="MRQMRWRVATSMTLAVAIFAVTGASAMPEPIHAIGHLDDPLTPT
                     ESIIVASSGAEGLPLPWNAAALPSSSILFPGIRPGSWMIYPNYCTMNFIFNESGSLAG
                     GGPYYIGTAKHCVQTQTGTDQFDASENVIGKHVVLGLSRKSGGAPQMVDIGTVAYATD
                     GASSLGNDYALVRINSSLERIISPSISVIGGPTSVYSASGSDMILTSGHGLGLGSTGN
                     GRPGQISAYNRYFPQGYGMILPVSFGDSGGPVRTINGAAVGNVTNIGVSDYFSPGIAF
                     GTRVGITGRWGLKVVTCRTNDPWPLAGCP"
BASE COUNT          636 a          969 c          846 g          641 t
ORIGIN      
        1 aaaatcagac caggaacgta acgccagccc acggttctcg ttgataggag caacaaggtg
       61 gaacaccctg atcaggggaa ctctctggca ggggaacgag attcaacgtc gaatccacag
      121 attgatgatc tctgggttag acgacaaggg ggggtatccg atggtgcttc ggggagggcg
      181 atcgcggtga gcggagagct cgcttatccg tcgtctgtgc cgagtgtgac cgaggccgac
      241 gccgaggcca ctgcgaagga aactgcccgc gtcgaggaga ccttcgagct cgtcaccaga
      301 gctaagaccg gcgatcagac cgcctttgcg gctctctatc gcatgcacgt ccagtcggtc
      361 taccgctacc tcgcgtacag actgcgtgat aactcgaccg ccgaggacct gactgctgaa
      421 gtcttcgtca gagcgctccg caagatcggt gacttcgagt ggcgcggcat cgacttctcc
      481 gcttggctgc tgcggatcgc ccgcaacatc cttctcgatc acgtgaagtc atcgggaacc
      541 cgtctcgaag tggttggtat ctctcccggt gacgtcaaag aaggaagttc tccgggaagc
      601 gacctcgagg ttctcgaccg cctcgatcgc gaggatctgc acaaagcgct caacaaactt
      661 cgtcccgagc atcaagaggt cttgtacctg aggttcttac aagggctctc cgcactcgaa
      721 accgctcagg cgatgggaaa gtcggaaggc gcagtcagag tcctgcagtt ccgggccctc
      781 aaacatctgg tcaagatcat gcagcaaggt caggaagacg cggggtgaaa cccgcacaca
      841 caaccaaagg cttctccagg ttaggcaagg gcctagccct gggcaccggt gcgcgctcga
      901 tccccatcga cgccatcatt cacaacctcg caacactctc gagtccatcg ctcagccagc
      961 tcgcggttga acgcatcgaa agcaaggtct tgggctccct gctgttcgat cctcctccac
     1021 cggcaagcaa cgacatcact gacgacgacc tcactagaca catctcggat gacgagatct
     1081 cggcgttcct tgcggaatca gtctctccgc cgatggtgcc ggttacttcc ccggaagcca
     1141 agccagtttc ccgccctcta gtccgcactc gtgcactcgg ctttggaact gcattcgccg
     1201 tccttgtctg ttcagcactc atcgtcgccg cgtccacagc gggcccgggg acactccttc
     1261 acccgctgcg gaccaagatc gaggcggtgc gggtctttgt ggcgccatcg ccccttccta
     1321 aagccaacgt ctacatggag atcgcccaga acaggctcga ttcgctcaga gcgatcactt
     1381 cggatcaacc tgaagtgatg aaacagaccc tgggtcagat gagcctggcc actcttcaca
     1441 gcctacggtt gatcgaaagt gccgacgatg gggcgaccaa gttcgatgct ctccgtcggt
     1501 tagatcgact cgcgaacgcc caaggcagaa ctctgattca gctgatgcgg agctgggatc
     1561 cccggatcga tgatgcactt tcttcttcgt atcgggttgc aacatatgtc gagacttgga
     1621 cccatgcgat cctgaccgga agatcgctgt cattcctcag cggtacattc agaccgcccc
     1681 caggtccgat aaccgtgacc gggcacggtc tagtccccat cacccatcca gccgatggac
     1741 gcaccggtga agggaaccgc cagtccacga cttctggtga tacccatggt gatacacagg
     1801 ggccggagcc cgagacctgc gacatggcgt ctgcgatgct ttgcgtatct gtgccagaca
     1861 cggtaactct gcctgcactc ccctcgttgt aactgacgac aagggtcctg gtcttgggcc
     1921 gggcatcaga gcaggaggaa cattggtctg catgagacag atgagatggc gagtcgcgac
     1981 atctatgact ctcgcagttg caatcttcgc agtcacgggt gcatccgcca tgcctgagcc
     2041 tatccacgcc atcgggcatc tcgatgaccc tctgacaccg accgagtcga tcatcgtcgc
     2101 gtcctcggga gccgaaggcc tgccacttcc atggaatgcc gcggcccttc cttcctcatc
     2161 gatactcttc cccggcatcc gtccgggatc ttggatgatc tatcccaact actgcacgat
     2221 gaacttcatc ttcaacgagt ccggctcact cgctggcggt ggcccctact acatcggcac
     2281 cgctaagcac tgcgtccaaa cccaaacagg gacggatcag ttcgacgctt ccgagaacgt
     2341 tataggcaag cacgtcgtac tcggcctatc tcgcaagtcg ggcggcgcgc cacagatggt
     2401 cgacatcgga acagtggcct atgccaccga cggagcatcc tctcttggga atgactatgc
     2461 gctcgttcgg atcaactctt cacttgagag aatcatcagc ccctcgatct ctgtcatcgg
     2521 gggcccgacc tccgtctact cggcgtcggg ttcggacatg atactcacgt caggacacgg
     2581 gctcggactc ggaagcacag gcaatggccg accgggtcag atctcggcct acaatcgtta
     2641 cttcccgcag ggctacggca tgatcctgcc cgtctccttc ggcgattcgg ggggcccggt
     2701 tcgtaccatc aatggtgccg ctgttggaaa tgtaaccaac atcggggtga gcgactactt
     2761 ctctcccggg atcgctttcg gaacgcgagt cggaataacc ggaagatggg gcctgaaggt
     2821 cgtcacctgt cgaaccaacg atccctggcc gctcgccggc tgtccttgag atctcttcgc
     2881 taggtacgtt tttcgacgta gtttttcgac cgatatagaa ggagtttcgc cgacttgcct
     2941 agaactcctt cattgtgggt ttacgccgcc ttgtcgcact ggctctagta tctgccgttt
     3001 tgtcggccta ctctttcgtc gcgcaggcca tcgctccccc acatccaacc gacagcttca
     3061 gttgcgactc agctacgaca gacgacttgg ag
//
LOCUS       DATDOZ010000003         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_265844, whole genome shotgun sequence.
ACCESSION   DATDOZ010000003 DATDOZ010000000
VERSION     DATDOZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265844"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VI541_00040"
     CDS             complement(<1..596)
                     /locus_tag="VI541_00040"
                     /inference="COORDINATES: protein motif:HMM:NF036798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HLF69329.1"
                     /translation="MTRESEFERRIGEYEREVGELQMQVKMLEEEAGGLRVKLADVPL
                     KIRKLEEELELGRSELLKSHDQGERMAAILREAREQIVALRDEIDKLTAPPNGYGVFL
                     GTNDDETVNVFTGGRKVRVNVSPELDASTFVRGQELMLNEALNVIAACDYEVVGEVVH
                     LKELIEENRALVFTHADEERVVQLSSQLAAQTLQAGDLL"
     gene            complement(729..1511)
                     /locus_tag="VI541_00045"
     CDS             complement(729..1511)
                     /locus_tag="VI541_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HLF69330.1"
                     /translation="MTLQEGDAVLLSDSKKRRYLIRLETGGSFHFHRGIVSHDDLIGR
                     KDGSRVRSTSGEILIAVVPTYEEYILKMPRGAQVVYPKDVATIIVKGDIYPGARVVEA
                     GAGSGALTLGLLRAVGPKGSVTTYEVREDFANNAQRNIASFLEDSSNHVLRMGDFYER
                     TQEPESSFDRTVLDLPEPWRCLEEAARILRSGGILTAYLPTVMQFHSLVGAMRASGSW
                     EMIQTVETLLRSWHVEERSVRPDHRMVGHTGFVTVARLVSTS"
     gene            complement(1508..2644)
                     /locus_tag="VI541_00050"
     CDS             complement(1508..2644)
                     /locus_tag="VI541_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLF69331.1"
                     /translation="MLLGRSFRIGQVLGIEVRLDGSWFLVSVLIAWSLASLFGANPAL
                     HGFSQGPLLVMGAMAALLFFATLLAHELSHGLVARRKGIEVESITLFIFGGVASLKGD
                     PQGPGDEFQIAIVGPATSLVCALVLFAVSRVAAASHVAPAAVIFSVIAASNVLLAAFN
                     MLPGFPLDGGRVFRSAIWKATGDLVKATRVAALAGKVVAALIAMRGIYIVVFEHDLFG
                     LVYVMMAMFLIQAGTATYRNVLVRRSVEGVSVADVMTPTPITVAGNVSVAEAVDSHFN
                     HHRHSAFPVVGYGDHIEGIVTLSAIEAIPAQQREHLVVRQVMLARQPTMITDPTEKMT
                     SLLDRVPANPAGRFMVMDAEKLVGILTPADISRYLQLHPAKERT"
BASE COUNT          572 a          878 c          819 g          548 t
ORIGIN      
        1 agaagatcgc ccgcttgaag ggtctgagca gccaattgcg acgagagctg aacgacccgt
       61 tcctcatcgg catgtgtgaa gacgagggct ctgttctcct cgatcaactc cttgaggtgg
      121 acgacttcgc cgacgacttc atagtcgcag gcggcgatca cattgagggc ctcgttgagc
      181 atgagttctt gcccacgaac gaacgtggat gcatccaatt ccggagaaac gttgacgcgg
      241 accttgcggc ccccagtgaa gacgttgacg gtctcgtcgt cgttggtgcc gaggaagacc
      301 ccatagccgt taggtggagc tgtgagcttg tcgatctcat cgcgcagagc cacgatctgt
      361 tcgcgcgctt cgcgcaggat cgcagccatc cgctctccct ggtcatgcga tttcaggagt
      421 tcgctacgac cgagctcgag ttcctcttcg agttttcgga tcttgagggg gacgtcggcc
      481 agcttgaccc tcaggccccc ggcctcttct tcgagcatct tcacctgcat ctgaagctcg
      541 ccgacctcgc gctcgtactc gcctatccgc cgttcgaact cagattcacg agtcatctgt
      601 cttcttgccc ccgttcgact gcagcaccct atctatcaca tcgcacccaa ggcccaagta
      661 taggcaccga gcacctctat gaatggattg tcggtcggtt ggtccgctta ctgaagtttt
      721 gaccgatttc agctggtgct gacgagtctt gccaccgtga cgaacccggt atgtccgacc
      781 atcctatggt ctggcctcac tgagcgctcc tcgacatgcc aagagcgcag cagtgtctcg
      841 accgtctgga tcatctccca tgaaccgctg gcgcgcatgg ctcccacgag tgagtggaac
      901 tgcatgaccg ttggcaggta ggccgtgagg atcccccctg atcgcaggat cctcgcggct
      961 tcttccaggc atcgccaagg ttcgggcaga tccagcaccg tgcgatcgaa cgaggactca
     1021 ggctcctggg tcctctcata gaaatcaccc atacgcagca cgtggttcga agagtcttcg
     1081 aggaaggaag cgatgttccg ctgggcattg ttggcgaagt cctccctcac ctcataggtg
     1141 gtgactgacc ccttcggacc cacggcccta agaaggccaa gtgtcagtgc gcccgaccct
     1201 gctcccgctt ccactacgcg cgctcccggg tagatgtctc ccttgacgat gatcgtcgcc
     1261 acgtctttcg ggtagacgac ctgtgcgccc cgaggcatct tcaggatgta ctcctcataa
     1321 gtgggtacga cagcgatcaa gatctcgcct gaggtggaac gcacccttga accgtccttg
     1381 cgaccgatca ggtcatcgtg agagacgatc cctctgtgaa agtgaaagga ccccccggtc
     1441 tccaatcgga tgaggtagcg ccgcttcttc gagtcagaga gaagtaccgc gtccccctct
     1501 tggagtgtca tgttcgctcc ttggcggggt gaagctgcag gtaacgagag atgtcggcag
     1561 gagtgaggat gccaacgagc ttttcagcgt ccatgaccat gaaccgaccc gctggattgg
     1621 ccgggacgcg gtcgagaagg ctcgtcatct tctcggtcgg atcggtgatc atcgtgggct
     1681 gacgagccaa catgacctgc cttactacga gatgctcacg ctgctgcgcg gggatcgcct
     1741 cgatcgcgct caaggtgacg atcccctcga tgtgatcgcc gtacccgacc accggaaagg
     1801 cactatgcct gtggtggttg aagtgggagt cgaccgcctc tgccacagag acgttgccgg
     1861 cgaccgttat cggtgtcggg gtcatgacgt cggcaacgct gactccttcg accgagcggc
     1921 gaacgaggac gttgcgatac gtcgccgtcc cggcttgtat caggaacatc gccatcatca
     1981 cgtagacgag cccaaagagg tcatgttcaa aaaccacgat gtagatgccg cgcatggcga
     2041 tgagtgcggc cacaaccttc ccggcaagcg cagcaacgcg agtagccttc acgaggtctc
     2101 cggtcgcctt ccatatcgcg gaacggaaga ccctcccacc gtcgagcgga aagcctggaa
     2161 gcatgttgaa agccgccagc aggacattgg aggccgcgat cacagagaag atgactgcgg
     2221 caggggccac gtggcttgca gcggcaacgc gtgagaccgc gaacagaact agcgcgcaca
     2281 ccagtgatgt cgcggggccg actatcgcga tctggaactc atctccgggc ccttgagggt
     2341 cgcccttcag tgatgctacg ccaccgaaga tgaatagcgt gatcgactcc acctctatgc
     2401 ccttgcggcg ggcgaccaaa ccatgggaga gctcgtgagc cagaagagtc gcaaagaaga
     2461 gcagggccgc catcgccccc atcaccagta gggggccctg cgagaaccca tgaagagcgg
     2521 gattggcgcc gaacagagac gccagggacc atgcgatgag taccgagacc aggaaccaag
     2581 aaccgtcgag cctcacctcg atacccagta cctggccgat ccgaaaactg cggccgagca
     2641 gcacctagat gctccttctc tcggcccctt gagcgcccat gaggcccttg gcgtcggact
     2701 gccagcggcg aaggccccga ttctcggccg caatgcgttc gggtgacgag tagaactcgc
     2761 cgaggctcct cttctgcacc atggctccag ggttgccagc caccgaaccc gccaatc
//
LOCUS       DATDOZ010000004         5111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907 k141_6120,
            whole genome shotgun sequence.
ACCESSION   DATDOZ010000004 DATDOZ010000000
VERSION     DATDOZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6120"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VI541_00055"
     CDS             complement(<1..208)
                     /locus_tag="VI541_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HLF69332.1"
                     /translation="MRVLHVFKDYYPPTRGGIEQHIHDVTHSLSGYDFAVLTSSRSRR
                     RITELDDGVRVVRAPELFRPVSTPV"
     gene            287..1267
                     /locus_tag="VI541_00060"
     CDS             287..1267
                     /locus_tag="VI541_00060"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF69333.1"
                     /translation="MGFWDGKKVCVTGGAGFIGSYLTELLVSDGAIVTVADNLSRGSL
                     ARLSAVATDIRLVDADLGTAEGALAATQGQDVVLNLAAKVTGIEYNRHHHADMFTANM
                     RIASSVIEAAVANRVDRFLVVSTACIYPHDAGVPTAETEGDRGTPEPTNEGYGWAKRM
                     AERLGRYHSAESGISVAICRPFNAYGPRDHWDEQTSHVIPALIKRVMDGEDPVIVWGS
                     GNQTRAFLHARDAARGMKLIAEKATSADPINIGHDDEVSIRDLAQTIVGIASSGAEIR
                     FDTSKPDGYPRRASDVTRLREVTGWVPDTPLGSGIEEMVAEYQLVRAGSL"
     gene            1277..2086
                     /locus_tag="VI541_00065"
     CDS             1277..2086
                     /locus_tag="VI541_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF69334.1"
                     /translation="MKHEVAQPSEMRPPERYRAVAYREILRALVTKELKVKYKRSILG
                     FFWSMITPIALTGIYLFVFIYVYKVPQKDFILFLLTGLLPWNYFNMSLMAATSSFVES
                     GSMIRKVYFPRLLVPIATVTANLVNFLVGLSLLLIVLVVSGRPIWLTLHWLLIAVVLE
                     TALAIGLSLILATWNVFFRDIGQLISIAMIGLFFATPVVYDISRVPAPFRPIILANPM
                     SSIIQTYRSSLFRAKSPDLGLLALGAAEVALIFVLGWLVYRRHANDLPKEV"
     gene            2086..2844
                     /locus_tag="VI541_00070"
     CDS             2086..2844
                     /locus_tag="VI541_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69335.1"
                     /translation="MSQASVAVTGLSEEYRRLTDREFSLKEAISRFRLPRRRSHIALQ
                     DIDISIPPGQTVGLIGENGSGKSTLLKCMAGILRPTSGSVLVSGPVSSLIELGAGFHP
                     ELTGRENVMVAGVLMGMTHDELLRKMETIVAFAELASMIDEPVRSYSSGMYVRLAFSL
                     AVHVEPEILLIDEALAVGDESFQRKCLSRVRDLEAQGRTIVFVSHDLELVRRVCRRTI
                     WLHEGRIAADGPTDEVISRYLAAEDLKSGGPEGP"
     gene            complement(2851..3858)
                     /locus_tag="VI541_00075"
     CDS             complement(2851..3858)
                     /locus_tag="VI541_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLF69336.1"
                     /translation="MSRVMLDSRMPIGGAGRYARDLIAGLAELDGTFEYKVLGSSGAG
                     TAAFTPWGRRSVAREARRWPADLIHGLHFELPRSKLPKVVTIQDLIPVEFPDSMPSPS
                     RRRVFRKILELSLARASCVIVPSDATASAVIRFGGRAANIRVIPLGVSDAFAPLQPGR
                     ADEVRSRVSGGRHYVAAIYHPKAHKNLGPLIAASCMWRSDLDLVLAGGGELPRQRRMH
                     ALTDVDDSALRDLYGAATLVVIPSLVEGFGLPALEALATGTPVIAGPGLASARYLAGA
                     VQMCDVTDPASIAAAVDALSHDKDRLNAMTSDGASAVADLTIEKMATLTSQVYRELLG
                     A"
     gene            3938..>5111
                     /locus_tag="VI541_00080"
     CDS             3938..>5111
                     /locus_tag="VI541_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLF69337.1"
                     /translation="MGASATLTPTLGLLEKIASRDANVAVIGLGYVGLPLALHACAAG
                     LRVFGLDDDPRRVARLQEGHTDIVDVDDELLGGAIDSVKLTVGGDPSVLANVDVVVIC
                     VPTPLADDLPDMSFVKAAAEQVAAHLRQGQLVILESTTYPGTTEEMLLPILETSGLKA
                     GIDFSLGYSPERIDPGNPTAKLENVPKLIGGIDDRSVESMAAFYGLFIEKTVRVSSPR
                     TAEMAKLLENTYRHVNIALVNEIAILCHDLGIDVWEVIAAASTKPFGFQPFYPGPGWG
                     GHCIPVDPAYLSWRVRQMGSTARFVELAREFNEKMPRYVVERIDEVLLSNGKTLKGSS
                     VLMIGVTYKADVADTRETPAVPIIEGLRKAGATTTFHDPLIASLETRHGTMQSIDLT"
BASE COUNT         1021 a         1576 c         1520 g          994 t
ORIGIN      
        1 tcacgggcgt cgataccggc ctgaacagtt cgggggcccg aaccacccgg accccatcgt
       61 ccaactccgt gatccgcctg cggctacggg aagaggtcag gacagcgaag tcgtagccgg
      121 atagcgagtg agtcacgtca tggatgtgct gttcgatccc acctctggtc ggtgggtagt
      181 agtccttgaa gacgtgcaga actctcatcg gtgcgcaggt tacagtcttg caggtcatat
      241 cctgcaagag tcggagttct gactccccta gcggaagggc gtcatcttgg gtttctggga
      301 tggaaagaag gtctgcgtca caggtggggc tggcttcatc ggttcctacc tcacagagtt
      361 gctggtgtca gacggtgcca tcgtcacggt cgcagacaac ctctcaagag gttccctcgc
      421 gaggctctct gccgtggcca cggacatccg actcgtcgac gcagatctgg ggacagccga
      481 gggggccctt gccgcaacac aggggcagga cgtcgtgctg aacctggcgg ccaaagtgac
      541 gggtatcgag tacaaccgcc accaccacgc cgatatgttc accgcgaaca tgcggatagc
      601 ctcatctgtc atcgaggcag ccgtcgccaa ccgagtggac aggttcctgg tcgtctcgac
      661 cgcgtgcatc tatccccacg atgccggggt ccccacagcc gagaccgagg gggatcgcgg
      721 cacaccagag cccaccaacg agggctacgg ctgggcgaag aggatggctg aaaggctggg
      781 ccgttaccac agcgcggaga gcgggatatc ggtcgccatc tgtcggccgt tcaacgccta
      841 cggccctcgg gaccactggg atgaacagac atctcatgtg atacctgccc tgatcaaacg
      901 cgtgatggac ggagaggacc ccgtcatcgt ctggggttca ggcaaccaaa cacgagcatt
      961 cctgcatgca cgagacgcgg cccgcggtat gaaactgatc gccgagaaag cgaccagcgc
     1021 tgacccgatc aacatcggcc acgacgacga ggtatccatc cgagatcttg ctcagacgat
     1081 cgtggggatc gcgtcctcgg gggccgagat ccgattcgat acctccaaac ccgacggtta
     1141 cccacgtcgt gcctcagacg tcacgcgact ccgtgaagtc acagggtggg ttcccgacac
     1201 tcccctagga tccgggatcg aagagatggt cgcggagtac caactcgtcc gcgccggaag
     1261 cctctaggtt caggccttga agcacgaggt cgctcaaccg agtgagatga ggcccccgga
     1321 acgataccga gcggtggcgt accgagagat cctcagagcc ttggttacga aagagcttaa
     1381 ggtcaaatac aagcggtcga tactcgggtt cttctggtcg atgatcacac cgatagccct
     1441 cacgggcatc tatctcttcg tattcatata cgtctacaaa gtgccgcaga aggacttcat
     1501 cctcttcctc ctgaccggtc ttctcccatg gaactacttc aacatgtcgc tcatggcagc
     1561 aacatcatct ttcgtcgaga gcgggtcgat gatccgcaag gtctactttc cgaggctgct
     1621 agttccgatc gcgacggtca cggccaacct ggtcaacttc cttgttgggt tgtcccttct
     1681 tctgatcgtg ttggtggtat ctggccgacc gatctggttg accctccact ggctattgat
     1741 cgcagtcgtg ttggagacgg cgctggccat cgggctatcc ctcatcttgg caacctggaa
     1801 cgtcttcttc cgcgacatcg gtcagttgat cagcatcgcg atgatcggcc tcttcttcgc
     1861 aaccccggtc gtctatgaca tctcgcgtgt tcccgctcca ttcaggccca tcatcctggc
     1921 caacccgatg tcttcgataa tccagacata taggtcgtct ctcttcagag cgaagtcgcc
     1981 agatctggga cttcttgcgc tgggagccgc cgaagttgcg ctgatcttcg ttctcggttg
     2041 gttggtctac cggcgtcatg ccaacgacct tcccaaggag gtctgatgag tcaggcctct
     2101 gtcgcagtaa ccggcctcag cgaggagtat cgacgcctca ccgatagaga gttctctctc
     2161 aaagaagcca tctcacgctt tcggctccca cgtcgaaggt cccatatcgc actgcaagac
     2221 atcgacatct cgatccctcc gggtcagacg gttggtctca tcggcgagaa cgggtcgggc
     2281 aagagcaccc ttctgaagtg catggcgggg atcctgcggc ccaccagcgg aagcgtcttg
     2341 gtttctggac ctgtctccag tctcatagaa cttggcgcgg gatttcatcc ggaactgacc
     2401 ggtcgagaga atgtcatggt tgccggtgtt ctcatgggga tgactcacga cgaactcctg
     2461 aggaagatgg aaacgatcgt cgccttcgcc gagttggcat cgatgataga cgagccagtc
     2521 aggagctact catcggggat gtacgtccgc ctggcgttct cgctagcagt acatgtcgaa
     2581 cccgagatcc tcttgatcga tgaagccctg gccgttggag acgaatcctt tcaacgcaag
     2641 tgcttgtcgc gggtcagaga cctcgaagcg cagggccgca cgatcgtctt cgtctcgcac
     2701 gacctagaac tcgtgaggcg ggtctgccgc aggaccatct ggctccacga gggccggatc
     2761 gcggccgacg gtccgaccga cgaggtgatc tcacgttacc tcgcggccga ggacctcaag
     2821 agcgggggcc cggaagggcc ctgaggatcc ttaggccccc agaagctcac ggtagacctg
     2881 cgatgtgagg gttgccatct tctctatcgt gagatcggca actgcgctcg cgccatcact
     2941 ggtcatcgcg ttcaagcgat ctttgtcgtg cgatagcgca tcgactgccg cagcgatgct
     3001 cgctgggtcg gtcacatcgc acatctgcac ggcccccgca aggtatcgag ccgatgccag
     3061 gcctgggcca gcgatcaccg gggtcccggt ggcaagggcc tcgagtgccg ggagaccgaa
     3121 cccctcgaca agtgacggga tcacgaccaa ggttgcggca ccgtaaagat cgcgaagggc
     3181 actgtcgtcg acatctgtca gcgcatgcat cctcctttgg cgtgggagct cacccccgcc
     3241 cgccaacact agatcgaggt cgctgcgcca catgcacgac gctgctatca gggggccaag
     3301 gttcttgtga gccttcgggt ggtagatggc cgcgacgtag tgacggcccc cggagacccg
     3361 agagcgaacc tcatcggctc gtcccggctg cagaggtgcg aaggcatcgg atacccccag
     3421 cgggatcact cggatgttcg ccgcgcgccc cccgaagcgg atgacagctg atgcggtcgc
     3481 gtcgctgggg acgatcacac acgatgctcg cgcgagggac agttcgagga tctttcgaaa
     3541 gacacgacgt cgtgaggggc ttggcatcga atctggaaac tcgaccggga tgaggtcctg
     3601 gatcgtcact accttcggga gcttcgagcg aggcagttcg aagtgaagac catggatcag
     3661 atctgctggc cagcgtcgag cctcgcgggc gaccgacctt ctcccccatg gagtgaaggc
     3721 ggctgtaccc gcccctgacg aaccgagcac cttgtactcg aaggtgccgt cgagctcagc
     3781 gaggcctgcg atcaggtccc gcgcgtatct accggcgccc cctatgggca tccgagagtc
     3841 gagcatcacc cgggacatga cggcgaccct acctcgcgaa ccgttctagc gcggctggcc
     3901 gtcggaaacc gacgccagat cgcgccagaa cggagacttg ggggcctcgg ctacactcac
     3961 acccactttg ggtctcttag agaagatcgc ttcacgcgac gccaacgtgg cggtgatcgg
     4021 cctcggttac gttggactcc cactcgcgct gcatgcttgt gcggcgggcc tgagggtctt
     4081 cggactcgac gacgatccac gccgagtcgc gcggctgcaa gaggggcaca ccgatatcgt
     4141 cgatgtagac gacgaacttc ttggtggcgc gatcgactca gtgaagttaa ccgtcggggg
     4201 cgacccatcg gtccttgcta acgtcgatgt cgttgtcatc tgcgtcccaa cgcctctcgc
     4261 cgacgacctt cccgacatgt cattcgtgaa ggcagccgca gaacaggtcg cggcccacct
     4321 ccgtcaagga caacttgtca tcctggagag cacgacctac cccggcacga ccgaagagat
     4381 gctgctaccc atactcgaga cctctggcct caaagcgggc atcgacttct ctcttgggta
     4441 ttcaccagag aggatcgacc ccggcaaccc gacggccaag ctagagaacg ttccgaaact
     4501 gatcgggggc atcgacgaca gatccgtcga gtcgatggcc gccttctacg gtctgttcat
     4561 cgagaagact gtccgcgtct cgtctcccag aaccgccgag atggccaagc tgctagagaa
     4621 cacctaccgg catgtcaaca tcgcgctcgt caatgagatc gctatcctct gccacgacct
     4681 cggcatcgac gtctgggagg tcatcgccgc agcgagtacc aagccattcg gtttccagcc
     4741 gttctacccc ggccctggat ggggcggcca ctgcataccc gtagaccccg cctacctatc
     4801 ctggcgcgtg aggcagatgg gttcgaccgc acgattcgtc gagctagcgc gcgagttcaa
     4861 cgagaagatg ccacgttatg tcgtcgagcg gatcgacgag gtccttctgt cgaacgggaa
     4921 gactttgaag gggtcgtctg ttctgatgat cggcgtcacg tataaggccg acgtcgcgga
     4981 caccagggag actcctgcgg tcccgataat cgaagggttg cgcaaggctg gggcgaccac
     5041 aacgtttcat gaccctctta tcgcatcgct agagacaagg catggcacga tgcagagcat
     5101 cgaccttacc g
//
LOCUS       DATDOZ010000005         2300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3668225, whole genome shotgun sequence.
ACCESSION   DATDOZ010000005 DATDOZ010000000
VERSION     DATDOZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668225"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VI541_00085"
     CDS             complement(<1..470)
                     /locus_tag="VI541_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69338.1"
                     /translation="MRFSDLYLLLLGMTASFASVLMYVSGITPLASLLLLLGVGFAVI
                     SYRRTVRSPRLLTVGFPASRSALRYRLDEVGYRLRDCAGPSAGPCPVMQGEACPYSSK
                     AFASAVIYLEAGSPDAPPCGSAVAAPELLIAEGSFEPASFGTGGGHIGANQAPD"
     gene            complement(627..711)
                     /locus_tag="VI541_00090"
     tRNA            complement(627..711)
                     /locus_tag="VI541_00090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(674..676),aa:Leu,seq:gag)
     gene            complement(736..2190)
                     /locus_tag="VI541_00095"
     CDS             complement(736..2190)
                     /locus_tag="VI541_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69339.1"
                     /translation="MSARKLLVFTSLAGLLAASGIVYMSRRQPQGPRPSAGPPQDPSG
                     EVTIAYPQDPVSLDPYSVQGDTSATKDLLRPVLPTLLTITPEMRYAPGLALRVPRGRD
                     ITTTPFSVTYHLDPAARWSDGSAMGAADVRATWQAIRQGTVASSRYSHVVDVKVDGPH
                     RVTVVFDTPQEGWRDLFSAGDFILPASLAGRGDRAEVMSTGAPISGGPFLLQAYEAGL
                     SLTYSSNPKWWRNGPGLKRVTVLIVPDDETAIGLFKQDEVDVIAATSRLNLRHRLSAA
                     GASVSARFGYSWWELVFNTSSGDVREPHLRRAIALSVDRQGFVEAFLSSEGRVLDSQV
                     PGSGPKLYADLHRDNKAASAELKLAKVPASTGRSTLTISAAVDSLGDLLERTIERALT
                     DLGLKVDLRDTSADELYGEWLPKGDFQIALVERRGSPALSAISADSAALRSQLTVLPL
                     LETQMIIGFRRNVKGPSANASTDGPLWNLHMWKG"
BASE COUNT          487 a          692 c          702 g          419 t
ORIGIN      
        1 ttatcggggg cctggttggc accgatgtgc cctccgcctg tgccgaacga cgcgggctcg
       61 aaggagccct ctgcgatcag caattccggt gcggcgactg ctgaaccgca ggggggcgca
      121 tcaggagaac ccgcctcgag atagatcact gctgaagcga aagccttgga cgagtatgga
      181 caggcctccc cctgcatgac ggggcacggc ccagcggagg gtcccgcgca atctcggaga
      241 cggtaaccaa cttcatcgag tcggtatcga agggcacttc tcgatgccgg gaaaccgaca
      301 gtcaacaatc tcgggctccg gacggtgcgc cgatatgaga tgaccgcgaa tccgacaccc
      361 aggagcagca ggagcgaggc cagcggggtg atccccgaca catacatcag gaccgacgcg
      421 aaggacgcgg tcatcccgag tagtagcaga tagagatccg aaaacctcat gggacagcct
      481 cctgaatcaa tcggtgtttc atctaaggat gggctcaaga aatgcccgag gatagggcca
      541 ttcggcccta tcgctaccta tctgcaggag ctggcgtcgg tagcgggttg aattccgccg
      601 gcaagatcga acaggagtga taagcgtgtc ggagcgggga tttgaacccc gatgatcttg
      661 cgatcactag gccctcaacc tagcgcgtct acctagttcc gccactccga ctggaacccg
      721 ggcattatgc catcactacc ccttccacat atgcagattc caaaggggcc cgtcggtaga
      781 agcgttggca gacgggccct tcacgttgcg gcgaaaccct atgatcatct gggtctccag
      841 cagaggcagc acggtcaact gcgaccgtag agccgccgag tcggcgctga tggcagagag
      901 ggccggagaa cctcgacgct cgacgagagc tatctggaag tcgcctttgg gaagccactc
      961 gccgtacagc tcatcagccg aggtgtcgcg cagatctacc ttgagaccta gatccgtcag
     1021 ggctcgctct atggtccgtt ccaggaggtc cccaagagaa tctaccgccg cgctgatggt
     1081 cagagtggat cgacccgtcg acgcgggaac cttcgccagc ttcaactcag ccgacgccgc
     1141 cttgttgtcg cgatgcagat cggcatacaa cttcggcccc gacccgggga cctgcgaatc
     1201 gagaacccgc ccttcggacg ataggaaggc ttcgacgaaa ccttgtctat ccacagagag
     1261 agcgatcgcc cgtcgtaggt gaggctccct gacatcgccc gaggaggtat tgaagacgag
     1321 ttcccaccat gaatatccga accgggcact cacgcttgcc cccgccgctg aaaggcgatg
     1381 ccgtaggttc agtctcgaag tggccgctat cacatccacc tcgtcctgct tgaagagccc
     1441 gatcgcggtc tcgtcgtctg gaacgatcag aacggtaact ctcttgagac caggcccgtt
     1501 acgccaccac ttgggattac tgctgtaggt cagagagagt cccgcttcgt aggcttgcaa
     1561 caagaacggc ccgccagaga taggggcccc cgtgctcatc acctctgcac ggtcgccacg
     1621 acctgctagc gacgcaggaa ggatgaagtc gccagcggag aagagatcac gccacccttc
     1681 ttgaggagtg tcgaaaacga cggtgactct gtgtggtcca tccaccttca cgtccactac
     1741 atgtgagtag cgcgaggacg ccactgttcc ctgtctgatg gcttgccacg tcgctcgaac
     1801 atccgcggca cccatggcgg acccatccga ccatcgagcg gctgggtcga ggtggtatgt
     1861 gactgagaat ggagttgtcg tgatgtctct accgcgtggg actcgcagtg cgaggccggg
     1921 cgcatatcgc atctcgggtg tgatggtgag aagtgtcggc aggacgggcc tcagaaggtc
     1981 cttcgttgcg gatgtatccc cctgtaccga gtaagggtcg agagatacgg gatcttgtgg
     2041 gtatgcgatc gtgacctcgc cgctaggatc ctggggcggc cctgccgaag gcctcggccc
     2101 ctgtggttgc ctgcgcgaca tgtagacgat ccccgacgcc gctaggagtc cagccaacga
     2161 tgtgaagacc agcagtttcc tagcggacat gtctgagctt gccgaggtcg aaggttccgt
     2221 ccggacgaac cgtgagcccc ttcacgtcca aagccgcgat gaaacggctt cgcagttcaa
     2281 tgagggggat caccgccagg
//
LOCUS       DATDOZ010000006         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2622497, whole genome shotgun sequence.
ACCESSION   DATDOZ010000006 DATDOZ010000000
VERSION     DATDOZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622497"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VI541_00100"
     CDS             complement(<1..594)
                     /locus_tag="VI541_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69340.1"
                     /translation="MKAPTKKTQTKASPAGKSKLKARESIKPKVSAKPKVSAKPKVSA
                     KPKPKTRQKPSVKVKTKAKAKVSPKPKLKPIPKPAPAKKPPAAAEPVVKPRPVLKLTP
                     IPKPKPVPVKKVRRVNFDAKTLATIQANLENERIDLLAQLEQIEQTGLAGPQAEGHGE
                     GGFDEDYADAGSATFEKELDLSLANNVTDLLGKIDKAL"
     gene            869..>3070
                     /locus_tag="VI541_00105"
     CDS             869..>3070
                     /locus_tag="VI541_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLF69341.1"
                     /translation="MRLILFEKVNSNSELPTGERDILDHWERSRTFDALRAKVAGGPR
                     WSFLDGPITANNPMGVHHAWGRTYKDAFQRFFAMTGHDQRFQNGFDCQGLWVEVQVEK
                     ELGFTSKRDIEAFGLERFIDLCKERVRKYSAIQTEQSIRLGMWMDWDNSYYTMSDENN
                     YTIWSFLKRCHERGLIYKGLDAMPWCPRCGTGISEQERKEGYVPVTDRSVYVRLPLRE
                     RPSEYLLVWTTTPWTLAANVAAAVNPELTYVKVRQGDDIYYLSKGRVDVLKSESKVRG
                     KPEVLAEMKGADLVGWTYDGPYDDLPAAREASLAHRIIPWDGVEESSGTGIVHIAPGC
                     GKEDFDLGRQLQLPTVAPIDEEGVYVAGFGPLTGLSALDVSEQVFGHLKEKSLYYKIE
                     PYEHDYPHCWRCGTALLFRAVDEWYINLDWRDEIRKVVDQITWIPDYGRELELDWLRN
                     MGDWMISKKRYWGLALPIFECDECGWFDVIGNREELEARAVQGWEQFQGDPDSPNSPH
                     RPWVDAIRIECGNCGALASRIPDVGNPWLDAGIVPYSTVGYNSDRGYWARWIPADFIV
                     ESFPGQFRNWFYSLLAMSTMMENIAPFKTLLGYALVRDEQGDEMHKSKGNSIEFNAAA
                     DEVGADVMRWLFCRHNPTLNLNFGYTSAHQIERKTFGTLWNSYLFFTNYAILDEFDPT
                     SPQIPPQDRPEIDRWILSNLQLLIQTAHLSLTNYSTYQFVREAEQFIDDLSN"
BASE COUNT          607 a          916 c          889 g          658 t
ORIGIN      
        1 gagggccttg tctatcttgc cgagcagatc ggtgacgttg ttggccagag acaggtcaag
       61 ctccttctcg aacgtggcgc tccccgcatc tgcatagtcc tcatcgaatc caccctcgcc
      121 gtgaccctct gcctgggggc ccgcgagacc cgtctgctcg atctgctcta actgcgctag
      181 taggtctatg cgctcgtttt cgagattcgc ttggatcgtg gctagcgtct tagcatcgaa
      241 gttcactcgg cgcaccttct tcacgggtac cggcttcggt ttgggtatcg gagttagctt
      301 gagtaccggt cttggtttca caactggttc cgccgccgcg gggggcttct tggcgggtgc
      361 tggcttcggg atcggcttga gcttgggttt gggggatacc ttggcctttg ccttagtctt
      421 gactttcact gaaggcttct gcctcgtctt cggcttgggt ttggcggaca ccttgggttt
      481 ggcggacacc tttggtttgg cggacacctt tggcttgatc gactctctgg ccttgagctt
      541 ggacttgcca gcggggctcg ccttggtctg agtcttcttc gtcggcgcct tcactggctt
      601 gcgccctgtt cgcgccttgg caggggcagc tcttcgcgtt gctggcttac ccttcaagcg
      661 cgacggcttc gacttcgtcc gagcgggcgc gaccttcttt ttcgtggaga ctggaggcgt
      721 cttgcgacct gaggaccttg gcataaaatc ggcgaacctt agcacgcgac gatatgcgag
      781 tcaaagaaat cgacacagcg atggtcgcgc gacgcgagat actcacatct agcgcacccc
      841 acgcccatat agttgatatg tcttccaagt gaggctcatc ttgttcgaaa aggtcaatag
      901 caactcagag ctccccaccg gagagcgaga catcctcgat cactgggaaa ggtcccgcac
      961 attcgatgct ctccgtgcaa aggtcgccgg ggggccccga tggtctttcc tcgacggacc
     1021 tatcacagcc aacaacccga tgggcgtgca tcacgcctgg ggacgcacct ataaggacgc
     1081 ctttcagcgc ttctttgcga tgaccggcca cgaccagaga ttccagaacg gtttcgactg
     1141 ccaggggctc tgggtcgagg ttcaggtcga gaaggagctc ggcttcactt ccaaacgcga
     1201 tatcgaggca ttcgggctcg agagattcat cgacctatgc aaggagcggg tgcgcaagta
     1261 ctcagccatc cagacggaac agtcgatcag gctcgggatg tggatggatt gggacaactc
     1321 ctactacacg atgtccgatg agaacaacta caccatctgg agtttcctta agcgttgcca
     1381 cgagcgcggt ctgatctaca agggcctcga cgcgatgcca tggtgtccac gctgtggaac
     1441 gggtatctca gaacaggaac gtaaagaggg ctacgtcccg gtgaccgacc ggtcggtcta
     1501 cgttcggctc ccgcttcgcg agagaccgag tgaatacctc ttggtctgga ccacaacacc
     1561 atggaccctg gccgccaatg ttgcggcggc tgtgaaccca gagctgacct acgtcaaagt
     1621 cagacagggc gacgacatct actacctgtc caagggaagg gtcgacgttc tgaagtcgga
     1681 gtcgaaggta cgcggcaagc ccgaagtcct agcggagatg aagggagccg acctcgtcgg
     1741 gtggacatat gacggcccct acgacgacct tccagccgcc agggaagcat cactggccca
     1801 ccgcatcatc ccttgggacg gcgtcgaaga gtcctctggc actggcatcg ttcatatcgc
     1861 cccgggatgt ggaaaggagg acttcgacct cggtcgccag ctacagctgc ccacggttgc
     1921 cccgatcgat gaagagggtg tctacgtcgc tgggttcggc cccctgaccg gcctctcggc
     1981 ccttgatgtc tccgaacagg tcttcggaca tctcaaggag aagagcctct actacaagat
     2041 cgagccctac gaacacgact acccacactg ctggcgttgt ggaactgccc tcctcttcag
     2101 agcggtcgac gagtggtaca tcaacctcga ctggcgcgat gagatccgca aggtcgtcga
     2161 tcagatcaca tggatccccg actacggccg cgagctcgaa ctcgactggc tccgcaacat
     2221 gggcgactgg atgatcagca agaagcgcta ctggggtctg gcacttccaa tattcgagtg
     2281 cgacgagtgt ggatggttcg atgtgatcgg gaaccgagag gagttagaag cgagagcggt
     2341 acaggggtgg gagcagttcc agggcgatcc cgacagcccc aacagtcccc acaggccatg
     2401 ggtggatgcg atcaggatcg agtgcggcaa ctgtggggcc ctggcgtcgc gcatcccgga
     2461 tgtcggcaac ccctggcttg atgcggggat agtcccctac tccacggtcg gctacaactc
     2521 ggaccgggga tattgggctc gttggatccc agccgacttc atagtggagt cgttccccgg
     2581 ccagttccgc aattggttct attcgctgct tgcgatgagc acgatgatgg agaacatcgc
     2641 ccccttcaag actctcctcg gctatgccct cgtccgcgac gagcagggcg atgagatgca
     2701 caaatccaag ggcaactcca tcgagttcaa cgccgccgcc gacgaggttg gggcagatgt
     2761 catgcgctgg ttgttctgcc gccataaccc gaccctcaat ctgaatttcg gttacacgtc
     2821 ggcccaccag atcgagcgca agacgttcgg cacgctctgg aactcatatc tcttcttcac
     2881 caactacgcg atcctcgacg agttcgaccc cacctcacct cagatcccgc cacaggatcg
     2941 accggagatc gatcggtgga tactctccaa ccttcaattg ctgatccaaa ccgctcactt
     3001 gagtctcacg aactactcca cgtatcaatt cgtccgagag gccgagcagt tcatcgacga
     3061 tctctcgaat
//
LOCUS       DATDOZ010000007         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3669473, whole genome shotgun sequence.
ACCESSION   DATDOZ010000007 DATDOZ010000000
VERSION     DATDOZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669473"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VI541_00110"
     CDS             <1..234
                     /locus_tag="VI541_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921206.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLF69342.1"
                     /translation="GIFEGIPSPFEATRYHSLVVKSDRLPVGLQITASTSDGVIMGLE
                     HERFPIYGVQFHPESILTTVGMNIIRNFLESVR"
     gene            complement(231..>2046)
                     /gene="pknB"
                     /locus_tag="VI541_00115"
     CDS             complement(231..>2046)
                     /gene="pknB"
                     /locus_tag="VI541_00115"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HLF69343.1"
                     /translation="VMTGKLYGERYEITGPIATGGMSEVFMARDHLLNRTVAVKVLDS
                     ALSRDRTFIERFRREAQAAASLNDPHIVSIFDWGAMGGDHYIVMEYVEGRNLRDLITT
                     EGPLTLERAAEIASDVCSALEVAHSQGIVHRDVKPANIAITPKGQTKVMDFGIARAVM
                     DGETVTQAGTVIGTANYLSPEQAQGLPVDARSDVYSTGVVLYEMLTQQVPFKADTAVA
                     IAYKHVKEDPLPPSSLNPEVSPPLDAVVMKALAKNPDNRYQSAEEMRLDLGRVLRGQA
                     VEATPLLSTDRTSLMGAGRATSVMESAEVPRGRKALAYTLIFALFASLLVLAVLGLFA
                     LFGKTTPTVAVPDVRGLSLTEAQRTLDAAGLKSIQDRLEFSESVSEGNVISQDPPDGR
                     KVPSGSKVNLVVSNGPERVEVPDLSGKTEEEARSALEDVGLTMGSVSSAFSDSVAQGK
                     VISQEPAAGVRVDRNRSVDVVVSGGKRTSRVPDVVGLDEAVARQRITDAGLNAVVREV
                     CNTSEKDGRVLDQDPPARQEVGEGSNVKITVNRAPTIPSVLDMTEANAKNRLETAGFK
                     VKVVHTHSLPGEDRVIAQDPVPSTVACKGDTVTITIDN"
BASE COUNT          364 a          602 c          655 g          425 t
ORIGIN      
        1 gggatattcg agggcatccc ctcgccgttc gaagcgaccc gctatcactc actggtagtc
       61 aagtccgaca ggcttcctgt gggactacag ataacggcgt cgacctcaga tggagtgatc
      121 atggggctgg agcacgagcg ctttccgatc tatggcgtgc agttccaccc agagtcgata
      181 cttacgacgg tggggatgaa catcatccgc aacttccttg agagcgttcg ctagttgtcg
      241 atcgtgatgg taacggtgtc gcccttgcat gcgacggtgc ttggcacggg gtcctgcgcg
      301 ataacgcgat cctcgcccgg gagcgagtga gtgtgcacga ccttcacctt gaagcccgca
      361 gtctctagac ggttcttggc gttggcttcg gtcatgtcga ggaccgaggg gatcgtcggc
      421 gcgcggttga cggttatctt cacgttggag ccttcgccca cctcctggcg cgctggagga
      481 tcttgatcta ggaccctgcc gtccttctcg gaggtgttgc agacctctcg aacgacagcg
      541 ttcaggccgg cgtcggtgat gcgctggcga gctacggctt catccaggcc aacgacgtcg
      601 ggcacccgag atgtgcgttt cccaccgctg acgaccacat cgacgctgcg gttgcgatcg
      661 actcggaccc ctgcggcggg ttcctgactg atgaccttgc cctgggcgac gctgtcgcta
      721 aaggctgatg agaccgaacc catggttagg ccaacgtcct cgagggcaga gcgcgcctct
      781 tcttcggtct tgccggagag gtcggggacc tcgacgcgct ccgggccatt ggaaaccacg
      841 agattcacct tcgaacctga ggggaccttg cgtccgtcgg gaggatcctg agagatgacg
      901 tttccctcgg agacggactc agagaattcg agtcggtcct ggatgctctt gagcccagcg
      961 gcatcgaggg tccgctgagc ctcggtgagt gacagccccc tcacgtcggg aactgcaacg
     1021 gtgggagtgg tcttgccgaa gagagcgaac agtccgagga cggcgaggac caggagactt
     1081 gcgaagagag caaagatcag cgtgtaagcg agggccttgc gtccccgtgg cacctctgcg
     1141 ctctccatca ccgacgttgc gcgtccggcc cccatcaacg aggtcctgtc tgtcgacaga
     1201 agaggggtgg cttcgaccgc ctgcccgcga aggacccggc caagatcgag ccgcatctcc
     1261 tcggcgctct ggtagcggtt gtcagggttc ttggcaagcg ctttcatcac tacggcatcg
     1321 agagggggtg agacctcagg attgagtgaa cttggtggga gagggtcctc cttcacatgc
     1381 ttgtatgcga tcgccaccgc ggtatcggct ttgaatggca cctgctgtgt cagcatctcg
     1441 tagagcacca ctccagttga gtagacatcg gagcgagcgt cgactggcaa accctgagcc
     1501 tgttctgggg agagatagtt ggctgtaccg atcacggtgc ctgcctgcgt gaccgtctct
     1561 ccgtccatga ccgcgcgtgc gatcccgaag tccatcacct tggtctgccc cttcggtgtg
     1621 atcgcgatgt tcgccggctt cacgtcgcgg tgaacgatcc cttgtgagtg agcgacttcg
     1681 agagcggagc agacgtcaga tgcgatctct gcagcgcgtt cgagcgtgag tgggccttcg
     1741 gttgtgatca ggtcccggag gttgcggccc tccacatact ccatgacgat gtagtggtcg
     1801 ccgcccatcg ccccccagtc gaagatcgac acgatatgtg ggtcgttgag actcgcagcg
     1861 gcctgtgcct cacgccgaaa cctctcgatg aatgtgcgat cgcgcgagag agcactgtcg
     1921 agtaccttga cggccactgt gcggttgagg agatggtcgc gggccatgaa gacctctgac
     1981 atcccaccgg tggcgatggg accggtgatc tcgtagcgct cgccgtagag cttgcctgtc
     2041 atgact
//
LOCUS       DATDOZ010000008         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907 k141_8941,
            whole genome shotgun sequence.
ACCESSION   DATDOZ010000008 DATDOZ010000000
VERSION     DATDOZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8941"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2537
                     /gene="uvrA"
                     /locus_tag="VI541_00120"
     CDS             <1..2537
                     /gene="uvrA"
                     /locus_tag="VI541_00120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLF69344.1"
                     /translation="PHCHNCGRPIARQSPQDIVDQISALKEGTKFHVLAPVVRGKKGE
                     YENLFEDLAKKGFTRAKVDGEVITLADKPRLQRYYKHDIDVIVDRLVAKPDIARRLTD
                     SIETALGLAEGLASVEILDSGEVLTFSQHAACTFCNISFEELAPRNFSFNTPYGACPR
                     CDGIGTHLELDPSLVIADESLSISEGVIAPWGGATYGYFDSLMSAIGERYGFSIETPW
                     KELSKAHRDVILSGTGGERVLVKYKNRHGRARQYNAAFEGVIPWLRRRHTTSESDSVR
                     ERYEQFFRQVPCPECKGARLRPESLAVTISDLSISQVCAKSVLDGEKFFGSLALTERE
                     HAIADRILKEVNARLGFLMDVGLDYLTIERGAATLAGGEAQRIRLATQIGSGLVGVLY
                     ILDEPSIGLHQRDNRRLIETLTRLRDLGNTLIVVEHDEETISVADHVVDIGPGAGEHG
                     GRIVYSGPLAGLLEERTSITGAYLSGRMKIPIPVERRKTNERMLVVKGASEHNLKGID
                     VAFPLGVFTCVTGVSGSGKSTLVNEILFKAVHRHLFRDARQLPGKHKRVLGLEHVDKV
                     IDIDQSPIGRTPRSNPATYTGVFDHIRTLFSKTEEARVRGYKPGRFSFNIRGGRCENC
                     AGDGQIKIEMYFLPDMYVTCEVCKGRRYNRETLEVHYKGKTVSEVLDMSVEEALEFFE
                     PIPQIARHMRTLFEVGLGYVKLGQPAPTLSGGEAQRVKLASELAKRATGRTLYILDEP
                     TTGLHFEDISKLLKVLHGLVTKGNSVIVIEHNLDVIKTADWVIDLGPEGGDLGGEVVV
                     KGTPETVASASASYTGQFLKRILERKATPVARRPAAAAKKVKTARR"
BASE COUNT          568 a          822 c          817 g          486 t
ORIGIN      
        1 aaccgcactg ccacaactgt ggccggccga ttgcacgaca gtctccgcag gacatcgtgg
       61 accagatctc cgcgctcaaa gagggaacga agttccacgt cctggccccc gtagttcgcg
      121 gcaagaaagg cgagtacgag aacctcttcg aggacctggc caagaagggc ttcacgcgcg
      181 ccaaggtcga cggcgaagtg ataacacttg ccgacaagcc gcggctgcag cgctactaca
      241 aacacgacat cgacgtgatc gtcgataggc tcgtcgctaa gccagacatc gctcgtcggc
      301 tgaccgactc gatcgaaacc gcgctggggc tcgctgaagg gctggcttct gtggagatac
      361 tcgattcggg tgaggtgctc acattctctc agcatgctgc ttgcaccttc tgcaatatct
      421 cgttcgaaga gttggctccg cgcaacttct ctttcaatac tccatacggt gcatgcccac
      481 gatgcgacgg gatcggcacc catcttgagc ttgatccatc actcgttatc gccgacgaga
      541 gcctttcgat ctcggaaggc gtcatcgctc catggggagg agcgacctac ggatacttcg
      601 acagcctcat gtccgcgatc ggtgagcgct acgggttcag tatcgagacg ccatggaagg
      661 aactgagcaa ggcccacaga gacgtcatct tgagtggcac cggcggcgag cgcgtgctgg
      721 tcaagtacaa gaaccgtcac gggcgagcaa ggcagtacaa cgccgcattc gagggcgtga
      781 tcccgtggct ccgaagacgt cacacgacct cggagtccga ctctgtacgg gaacgctacg
      841 agcagttctt cagacaggtg ccctgtcccg aatgcaaagg ggcgagactt cgcccggagt
      901 cactggccgt gaccatcagc gacctcagca tctcccaggt ctgtgcaaag tccgtgctcg
      961 acggcgagaa gttcttcggc tcactggcac tcacggagcg tgagcacgcg atagccgacc
     1021 ggatcctcaa agaggtcaat gcgcgcctcg ggttcctcat ggacgtcggg ctcgactacc
     1081 tgaccatcga acgtggcgct gcaactctcg ccggtggcga ggcccagcgc atacgactgg
     1141 ccactcagat cggcagtgga ttggtcggag tcctctacat actcgacgaa ccctcgatcg
     1201 ggctgcatca gcgtgacaat cgtcgcctca tagagaccct gacgcgactg cgagaccttg
     1261 ggaacaccct gatcgtcgtc gaacacgatg aagagacgat ctcggtggcc gatcatgtgg
     1321 tcgacatcgg tccgggggcc ggtgaacatg ggggccggat cgtctactca ggccccctcg
     1381 ccgggttact cgaggagcga acatccataa cgggcgcata cctgtcgggc cgtatgaaga
     1441 tcccgatccc ggtggagcgt cgtaagacca acgaacggat gctcgtcgtc aagggggcct
     1501 cggagcacaa cctcaagggt atcgacgtgg ccttcccgct cggtgtattc acctgcgtca
     1561 ccggggtttc gggctcgggt aagtcgacgc tggtgaacga gatcctcttc aaggcggtgc
     1621 atcgtcatct attccgagat gcccggcagc ttccgggtaa gcacaagcgg gtgcttggtc
     1681 tggagcatgt cgacaaggtc atcgacatcg atcagtcacc gatcggtcgc acgcctaggt
     1741 cgaatccggc gacatacacc ggcgtcttcg accacatcag aacgctcttc tccaagacag
     1801 aggaagcccg ggtccgcggt tacaaacctg ggcgcttctc attcaacatc cgaggtggac
     1861 ggtgtgagaa ctgtgcagga gacggtcaga tcaagatcga gatgtacttc ttgcccgaca
     1921 tgtacgtcac gtgcgaggtc tgcaagggcc gccgctacaa ccgggagacc ctcgaggtcc
     1981 actacaaagg caagaccgtc tccgaggtgc tcgacatgag cgtcgaagag gcgctcgagt
     2041 tcttcgaacc gatcccacag atagcgcgcc acatgcgcac tctcttcgag gtcggtcttg
     2101 ggtatgtgaa gctcggccag cccgcgccca cgctctcggg aggcgaggca caacgtgtga
     2161 agctcgccag cgaactggcc aagcgcgcga ccggtaggac cctctacata ctcgacgagc
     2221 caactaccgg actgcacttc gaggacatat cgaaactgct caaggtcctt cacggtttgg
     2281 tcacaaaggg caactcggtg atcgtcatcg aacacaacct cgacgtgatc aagacagcag
     2341 actgggttat cgacctcgga ccggaagggg gtgacctggg cggcgaggtc gtcgtcaagg
     2401 gaacccctga aaccgttgcc tcagcctcgg cgtcgtatac gggacagttc ctcaagcgga
     2461 tcctcgagcg aaaggctacg ccggtggcgc gacgcccagc cgctgcagcg aagaaggtga
     2521 agacggcccg gcgatgaggc ctcggcgcgt gctcgctccg ctcgattggg cgttgaccgg
     2581 tcccgttagg gggctacgcg gcgacctcca ggaactgttg ctcttccccc tggtcaggcg
     2641 attcgccaag atgcgggtcc gtggcgagca caacctcgcg acggcggggc cat
//
LOCUS       DATDOZ010000009         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2885713, whole genome shotgun sequence.
ACCESSION   DATDOZ010000009 DATDOZ010000000
VERSION     DATDOZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885713"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..2097
                     /locus_tag="VI541_00125"
     CDS             298..2097
                     /locus_tag="VI541_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HLF69345.1"
                     /translation="MPVPGKTLAEIFVNRVAKTPDFVALRQKEDGQWKPWTFREFGQQ
                     AREVAQGLIALGLTKGGGVALLSSNRAQWHISDAGIALSSGVSVPIYVTNSPSQVAYV
                     IGHSDSKLAIVENQDQLEKILKVREELPKLEKVIVISGEGADQSDFVISFEELKRLGR
                     EFEKANPSLLDETIRSITADDLATIVYTSGTTGDPKGAMLTHGGFAWTLNSLNEVLTF
                     HDGTERVLSYLPLSHIFERLTSDWGGIAFGVEVWFAESIDKLLANLQECKPTFFIGVP
                     RVYEKFYMGVKAKVAAHEKRELVEKALALGAKKVAAKEGGKSFGLVDSLKLALFDKLI
                     LSKLRAGLGMENVRFAITGAAPISPEIIRFVHSMGIDLVEGYGQTEDNAPTSVNPPGR
                     ARIGTVGPPIPGLEVKFDADGEILVRGPNVFRGYFKNETATKESFTEDGFLRTGDVGE
                     LDEAGYLRITDRKKDLIKTAGGKYIAPQVIEGKLKFEPLIGQAVVIGDRRPFPTALLT
                     LDPDVAPAWAKGQGIDFIDVTELPGNPKVQEAVSAIVAKVNDGLSHAEQIKKWTLLPR
                     DFTQDAEEVTPTLKVRRKIIAEKYADAIEKMYS"
BASE COUNT          474 a          621 c          653 g          396 t
ORIGIN      
        1 ggcgaacgga accagacggc gacggatggt gcgatgagga cgtgggtgtc acgggggcgt
       61 tgcccgcggg ttcgccgccg ggtgtgggag cggtcttcgt acatgccaca agaatcgata
      121 ggaccatcaa cgcagacgcg aatcgtttca tggcgcgagc ctagtgggtt gacccaggcg
      181 acgtttgctc ccccttcagg tggcggggga taatcgagag aacaacgctc ttcggcctcc
      241 gctatgagat ggtggccccg cactcgtaga cttcaacgcc aggaaagggg accctagatg
      301 ccagtaccgg gtaagactct tgctgagatc ttcgtcaatc gcgtcgcaaa gacacccgac
      361 ttcgtcgctc tgcgtcagaa ggaggacggt cagtggaaac cctggacatt ccgtgaattc
      421 ggccaacagg ctcgcgaagt cgctcagggc ctcatcgccc ttgggctgac caaggggggc
      481 ggcgtagccc tcctctcatc gaacagggcg cagtggcaca tcagcgacgc cgggatagct
      541 ctgagcagtg gcgtgagcgt tcccatctat gtgacgaact cgccatcaca ggtcgcttac
      601 gtcatagggc actcggactc aaagctggcc atcgtcgaga accaggatca gttggagaag
      661 atcctcaagg ttcgcgagga gctacctaag ctcgagaagg taatcgtcat cagcggtgag
      721 ggtgccgacc agtcagactt cgtcatctcg tttgaagagt tgaagcggct cgggcgcgag
      781 ttcgaaaagg cgaacccatc cttgctcgat gagaccatcc gctcgatcac agccgatgat
      841 ctcgccacga tcgtctacac atctgggacg accggggacc ccaagggcgc gatgctgact
      901 catggcggtt tcgcatggac actcaacagc ctcaacgaag ttctcacctt ccacgacggc
      961 accgagcgtg ttctgtctta cttgcccctc tcgcacatct tcgaacgact cacatctgat
     1021 tgggggggca tcgcgttcgg cgtcgaagta tggttcgccg agtcgatcga caaactgctg
     1081 gccaatcttc aagagtgcaa gcccacgttc ttcatcggcg tgccgcgcgt ctacgagaag
     1141 ttctacatgg gcgtcaaagc caaggtcgcg gcgcatgaga agcgggaatt ggtcgagaag
     1201 gccctcgccc tcggagccaa gaaggtggcg gccaaagaag ggggtaagtc cttcggtctt
     1261 gtggattcac tgaagctcgc cctcttcgac aagctgatcc tttcgaagtt gcgcgcagga
     1321 cttggtatgg agaatgttcg cttcgccatc acaggcgcgg cccccatatc tccagagatc
     1381 atccggttcg tccactcgat gggcatcgat ctcgtcgagg gctatggcca gaccgaggac
     1441 aacgcaccaa cctcggtcaa ccctcctggg agggctcgca tcggcactgt gggcccaccg
     1501 atcccggggt tggaagtcaa gttcgacgca gatggcgaga tcctcgtgcg ggggccgaac
     1561 gtcttccggg gttacttcaa gaatgaaacc gcgacgaagg agtcgttcac cgaagatggc
     1621 ttccttcgca ccggcgatgt cggtgaactc gacgaggcgg gttaccttcg gatcacggat
     1681 cggaagaagg acctcatcaa gaccgcgggc ggaaagtaca tcgcgccgca ggttatcgaa
     1741 ggcaaactga agttcgaacc gctcatcggt caggcggtgg tcataggaga ccgccgtcca
     1801 ttcccgacag cgctattgac actagatccc gatgttgctc cggcatgggc gaagggccag
     1861 gggatcgact tcatcgatgt aaccgaactg cccggcaacc cgaaggttca ggaggccgtt
     1921 agcgccatcg tggcgaaggt caacgatggc ctgtcgcacg cggagcagat caagaagtgg
     1981 acgctacttc ccagggactt cacgcaggac gctgaagagg taacaccgac cctcaaggtg
     2041 cggcgcaaga tcatcgccga gaagtacgcc gacgccatcg agaagatgta cagctgacaa
     2101 ccaaggatcc ccaaccatgc cgctctatat ggaccgtcac gacc
//
LOCUS       DATDOZ010000010         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2102617, whole genome shotgun sequence.
ACCESSION   DATDOZ010000010 DATDOZ010000000
VERSION     DATDOZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102617"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..160
                     /locus_tag="VI541_00130"
     CDS             2..160
                     /locus_tag="VI541_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HLF69346.1"
                     /translation="MRPNTRSGAWITTPNDSSVLAPLPGSFLTLTTCHPKGSAAKRLI
                     LRAELVST"
     gene            complement(178..1344)
                     /locus_tag="VI541_00135"
     CDS             complement(178..1344)
                     /locus_tag="VI541_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF69347.1"
                     /translation="MAVAERILHLRPIIDLRSDASLVKAAQSGAEDAAEALARRYYGK
                     VHSFVSYMTYSSASHPDDLTQEVFARAWGALPRFNGRYQFSAWLLRIARNLMIDEARR
                     RLRAPTLTDPVDLEKLEPIDPTDHVWEAVRSDATARSVREVLADMPMRQRTVLVLREI
                     EGLSYSEIAAVMGTNDRAIEGILRRARERFRTEGRGALALAPPGFMRRGLNEILRLAR
                     IPDTSSLSYGFQMVATAAVAGIIAVSSVAPPPVGPPTARIASAAAMTADPIIAETPPV
                     VAGRSQVATPIKKHLPDVLQTTENQIQPIDETAPPLLGIETSVIKVRIFGGETIRVFG
                     DIAAALPASIDLAVGPIGPVSNEPTGSSVPLTGLPEIIQSYLPLPRALMRIRTK"
     gene            complement(1479..2189)
                     /locus_tag="VI541_00140"
     CDS             complement(1479..2189)
                     /locus_tag="VI541_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69348.1"
                     /translation="MLKVSNLSVFYGGVQVLKDVAFEVRPGEVVTIIGANGAGKTTTL
                     KTTTNVSELYKTVKGEIEFEGERIEKLPAHKISRLGMAHVPEGRKVFPNQSVEDNLIL
                     GAYHRKDKEIAKDVAQQYERFPRLGERRGQKAGLMSGGEQQMLAIARALMSRPRYLLF
                     DEPSMGLAPLIVKQVFEIINQLKSEGKTILLVEQMAFQALGIADRAYVLETGRIIKEG
                     TGAELLNDPAVKEAYLGA"
     gene            complement(2182..>2945)
                     /locus_tag="VI541_00145"
     CDS             complement(2182..>2945)
                     /locus_tag="VI541_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69349.1"
                     /translation="AKSMSKVFGGLRANDQIDMVVHRGTVHSLIGPNGSGKTTFINTV
                     TGVYTADEGLVEFMGERCDDKPLFTNVDRGMGRTFQNLQLWRRMTVLENAMVGLHSRM
                     HSSLLACMVRTPRQRAEEARVTARAMGLLKFCGIDSYSHLPAGQLPYGPQRLLEIARA
                     LALDPDFLILDEPAAGLNPAESHELTNLIREIAKTGITVFLIEHHMDIVMNVSDRITV
                     LDFGSRIADGTPEQVQSNPRVLEAYLGGEPETADA"
BASE COUNT          528 a          874 c          942 g          601 t
ORIGIN      
        1 agtgcgtccc aacacgcggt cgggggcttg gataacgact ccgaacgact catccgtatt
       61 ggcgcctctt ccgggatcgt tcctaacgct cacgacatgt caccccaagg gaagtgcggc
      121 gaagcgactc atcctgcggg ccgagttggt gtcgacctag gggccatctc gcgaggccta
      181 cttcgtccgg atcctcatca gggccctggg caggggcagg tatgactgga tgatctcggg
      241 gagccccgtt aggggaaccg atgatccggt gggctcgttc gacactgggc cgatcgggcc
      301 aacggcgagg tcgatcgaag cagggagcgc cgccgcgatg tcgccgaata ctctgatggt
      361 ctcaccaccg aagatccgca ctttgatgac cgaggtctcg ataccgagaa ggggtggcgc
      421 cgtctcgtct ataggttgga tctggttctc ggtagtctga aggacatcgg gcaggtgctt
      481 cttgatcggt gtcgcaacct gagagcgccc ggcgacgacg ggcggagttt cagcgatgat
      541 gggatcggcg gtcatggccg cggcagacgc tatgcgtgcg gtcgggggcc ccaccggcgg
      601 cggggctacg gacgagaccg cgatgatccc agcaactgcg gctgtcgcca ccatctgaaa
      661 tccgtaggag agcgatgaag tgtcggggat gcgggcgagt cgaaggatct cgttcagccc
      721 ccgtcgcatg aatccaggag gtgccagtgc caatgcgccc cggccttcgg tacggaaccg
      781 ttcgcgggcc cggcgcagga tcccctctat cgctcggtcg ttggtgccca tcaccgcagc
      841 gatctcggag taggagaggc cctcgatctc gcgaaggacc aatacggtgc gctgtctcat
      901 cggcatgtcg gcgagaacct ctcgcaccga gcgcgccgtc gcgtcgctac ggactgcttc
      961 ccacacgtga tccgttgggt ctatcggctc gagtttctcg agatcgacag ggtcagtcag
     1021 cgttggggcc cgaaggcgtc tgcgggcctc gtcgatcatg aggttccggg cgatccgcaa
     1081 tagccacgcg ctgaactggt atcggccgtt gaatctcggc agggctcccc acgcgcgtgc
     1141 gaagacctcc tgggtcagat cgtcggggtg cgacgccgat gaataagtca tgtacgagac
     1201 gaaagagtga accttgccgt agtaacgacg ggcgagggcc tcggcggcat cctcggcccc
     1261 cgactgggcg gccttgacca atgaggcgtc cgaccgaaga tcgatgatcg gcctgaggtg
     1321 aagtatgcgt tcggctacag ccattcggtt ccccccgcat acgacgcgtt gcaacgcgtc
     1381 gagtcattat ccagggtagc accggatgtt ctcggttaga aggtggtgga gggtgaactt
     1441 ccggcaaacc ttcgcaccag aacgcgaagc ctcgcgtctc aggcccccag gtacgcttcc
     1501 ttgacggctg gatcgttgag cagttcggct ccggttccct ctttgatgat gcgcccagtc
     1561 tcgaggacat acgcgcggtc cgcgatcccg agggcctgaa acgccatctg ctcgacgagc
     1621 aggatggtct ttccctcaga tttcaactga ttgatgatct cgaatacctg cttgacgatc
     1681 agcggagcca gtcccatcga gggctcatcg aagaggaggt agcgcggcct gctcatcagc
     1741 gctcgtgcga tggcgagcat ctgctgttcg ccgcccgaca tcaacccggc cttctgcccg
     1801 cgtctctcgc cgagccgcgg gaatcgttcg tactgctgcg cgacgtcctt agctatctcc
     1861 ttgtccttgc ggtggtaggc accgaggatg aggttgtcct cgacgctctg gttcgggaag
     1921 accttgcggc cctcggggac gtgggccatg ccgagtctcg atatcttgtg cgctggcagt
     1981 ttctcgatac gctcaccctc gaactcgatc tcgcccttaa ccgtcttgta cagctccgag
     2041 acattggttg tggtcttcag agtggtcgtc ttgccggcac cgttagcacc gatgatggtg
     2101 acgacctcgc ccggacgaac ctcgaatgcg acatccttga ggacctgtac gcccccgtag
     2161 aagacgctga ggttagaaac cttaagcatc tgctgtctcc ggctctccac cgaggtatgc
     2221 ctcgagaacc ctggggttgc tctgcacctg ctcgggggtc ccatctgcta tgcgagatcc
     2281 gaagtccaaa accgtgatgc gatccgagac gttcatgacg atgtccatat ggtgttcgat
     2341 gagaaagacg gtgatgccag tcttggcgat ctcgcggatc aggttcgtga gctcgtgact
     2401 ctcggccggg ttgaggcctg ctgcgggttc atcgaggatg aggaagtcgg ggtcgagcgc
     2461 gagtgcccga gcgatctcga gaagacgttg ggggccatat ggcagctgcc ccgcaggcag
     2521 gtgagagtag ctatcgatcc cgcagaactt gagaagaccc atcgcacggg cggtcacgcg
     2581 cgcctcctcg gccctttggc ggggtgtccg taccatgcat gcgagaagac tcgagtgcat
     2641 ccgcgagtgc agtccgacca tcgcgttttc gagcactgtc atgcgccgcc agagctgaag
     2701 gttctggaat gtgcggccca tcccgcggtc aacgttggtg aagagaggct tgtcgtcgca
     2761 gcgctctccc atgaactcga ccaatccctc atctgcggtg tacacaccgg tgacggtgtt
     2821 gatgaaggtc gtctttccgg agccgttcgg gcctatgagt gagtggacgg ttccgcgatg
     2881 aacgaccatg tcgatctggt cgttagcgcg aagtcctccg aagaccttgc tcatcgactt
     2941 tgcct
//
LOCUS       DATDOZ010000011         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3674368, whole genome shotgun sequence.
ACCESSION   DATDOZ010000011 DATDOZ010000000
VERSION     DATDOZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3674368"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="hemE"
                     /locus_tag="VI541_00150"
     CDS             complement(<1..826)
                     /gene="hemE"
                     /locus_tag="VI541_00150"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HLF69350.1"
                     /translation="MTATQLDRFSTAAGGGRPDATPIWFMRQAGRVLPRYRELRERYD
                     FQTLSSDPALTAEVTMLPLEVLPVDAAILFADLMTPLAAMGIDYSIVEGVGPVVAHPL
                     TDADRIGRIELRPTVEAIPALFEAIKLIRKELAHGIPLIGFAGAPFTIASYLIEGRGS
                     RDLAKTKRLMFSDDPSWQMLMEKLTNLLIDYLTEQVNSGVQAFQLFDSWVGVLSPHDY
                     REYVLPFSRRIFAQTKQLGVPRIHFGTGNATLLADMASAGAEVMGVDWRVPLDHARQ"
     gene            999..1742
                     /locus_tag="VI541_00155"
     CDS             999..1742
                     /locus_tag="VI541_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69351.1"
                     /translation="MQAKIARKMTLWLAVVMATAVMNLASASEVTVTNYTSNYDASQV
                     HDSSGLGSAQSSAVADDQGNFDLTAATTSSTPVGVVAPFPGTIAGLYTPTYQFKYMVG
                     ANQAYALSYMYSYVPVAPVGGGTWTIRASIADLLVALDHDQFDMRNPAEVRPSRGDAQ
                     AKVRLNVTFEPAWNCPTCGSNKFFRTSEDLYHWDTSAVIEGQIPHTAANRGSLLIEVT
                     MEGFSEAVGNAYGNLRLKGKLASIVVTDA"
     gene            complement(1750..>2148)
                     /locus_tag="VI541_00160"
     CDS             complement(1750..>2148)
                     /locus_tag="VI541_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69352.1"
                     /translation="AMCNGLAQYCLNLGLEYAKTRTAFDRPIGKNQYVQGHLVDSLVE
                     LKASRLLTNDCAWRYDRGEGVIQESSIAKLYASEMVNRVAYRMIQVYGGMGWMREVPL
                     ERIYRFVRIFTLVEGTSEIQKYIIAKTLGL"
BASE COUNT          431 a          634 c          654 g          429 t
ORIGIN      
        1 tctggcgcgc gtgatcgagc ggcacccgcc agtcgacccc catcacctcc gcgccggctg
       61 aagccatgtc ggcgagcagt gtggcgtttc cggtaccgaa gtggatccga ggaactccta
      121 gttgcttcgt ctgcgcgaag atcctgcgac tgaacggaag aacgtattcg cggtagtcat
      181 gtggcgagag gactccgacc caactgtcga acagttgaaa cgcctgcacc ccggagttga
      241 cctgttccgt caggtagtcg atcagcaggt tggtgagctt ctccatcagc atctgccacg
      301 aagggtcgtc cgagaacatc agccgcttcg tcttcgccag gtcacggctg cctcggccct
      361 cgatcagata actggcgatg gtgaaggggg cccccgcgaa accgatgagt gggatcccgt
      421 gggcgagctc cttacggatc agtttgatgg cttcgaacag cgcagggatc gcctcaaccg
      481 tcggcctgag ctcgatccgg ccgatcctgt cagcatcggt caaggggtgc gcgacgaccg
      541 ggccgacgcc ttcgacgatg ctgtaatcga tacccatcgc tgcgaggggg gtcatgaggt
      601 ccgcgaagag gatcgctgca tcgaccggga gaacctcgag aggaagcatc gtgacctcag
      661 ccgtgagagc tgggtcggag gagagggtct ggaagtcgta gcgctctcgc aactccctat
      721 accggggcag cacgcgcccc gcctgacgca tgaaccagat cggcgttgcg tctggtcggc
      781 cgccgcctgc ggctgttgag aatctatcga gctgagtggc agtcatgtga gggacattat
      841 cgccccgagt ggtgggacga gttgctggct acctggccaa acgaacgccg gatgcccttc
      901 tttaggaata aagaagggca ggaaagcagg aaagacctcc ccggtgtcga agatttcaga
      961 ccagccgaga ctgctcggct tgagccgcag gagatggatt gcaagcaaag attgcgagga
     1021 agatgacgct gtggctcgct gtggtgatgg ctaccgcagt catgaacctc gcgagcgcgt
     1081 ccgaggtgac agtgaccaac tacacatcca actatgacgc atctcaggtt cacgactctt
     1141 ccgggctggg cagcgctcag tcgagcgccg ttgctgacga ccagggcaac ttcgacctca
     1201 ccgcggccac gacgagctcc acaccggtgg gagtagttgc cccctttccg gggaccatag
     1261 ccgggctcta tacgcccacg taccagttca agtacatggt cggggctaat caggcatatg
     1321 ccctctctta catgtactcg tacgtacccg tagctcccgt tggtggtggg acttggacca
     1381 tcagagcttc gatcgcggat ctgctagtgg ccctcgacca tgaccagttc gacatgcgga
     1441 atcctgccga ggtcaggccc tcgcgaggcg acgcgcaggc taaggttcgg ttgaatgtga
     1501 cattcgaacc ggcttggaac tgtccgacct gcgggtcgaa caagttcttc agaacgtccg
     1561 aggatctgta tcactgggat acgtcggcgg ttatcgaagg acagataccc catacggctg
     1621 cgaatagggg ttcgctcctc atcgaggtta cgatggaggg cttctccgag gccgtcggca
     1681 atgcgtatgg gaacctcaga ctcaaaggga agcttgccag catcgttgtc acggacgcgt
     1741 gatcctggtc tacagcccca gcgtcttggc gatgatgtat ttctggatct ctgacgttcc
     1801 ctcgacgagc gtgaagatcc tcacgaatct gtagatcctc tcgagcggga cctctcgcat
     1861 ccaccccatt cccccatata cctggatcat ccggtacgcg acccggttca ccatctcgct
     1921 cgcgtagagc ttggcgatgg atgactcctg gatcacgccc tcgccgcggt cgtagcgcca
     1981 agcgcagtcg ttcgtaagta accgagacgc tttcagttct acgagtgagt ccaccagatg
     2041 cccctgcacg tactggttct tgccgatcgg gcggtcgaag gcagtcctag tcttggcgta
     2101 ctccaacccg aggttgaggc agtactgagc tagaccgttg cacatggc
//
LOCUS       DATDOZ010000012         2663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3675186, whole genome shotgun sequence.
ACCESSION   DATDOZ010000012 DATDOZ010000000
VERSION     DATDOZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3675186"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VI541_00165"
     CDS             complement(<1..468)
                     /locus_tag="VI541_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69353.1"
                     /translation="MRRGYRLAIKVAASSAMTLAAISDDEDVLAAITIGGEEIIQSAS
                     RAIQTHHLPVFEMQDDLYSTSLGPTVFQSVIPLEWQAWRLARYFGPDDRGYKVVGLAV
                     ESSQRATSVRQILSDALSLRGVALVASSAGPEAAIEELAGASPSAVVVSGSASY"
     gene            complement(623..1093)
                     /locus_tag="VI541_00170"
     CDS             complement(623..1093)
                     /locus_tag="VI541_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLF69354.1"
                     /translation="MNPNTQIEELLIVGTGPVARALVAIGEPMGYHVRVAAGPQTPRV
                     GEFDGADEVIVTPESTDVEAIRPSERTTVVVCAEDREFSKRVVRSLASTRVPYLGMLD
                     DAPELLETLRADGLSHEALERIHIPMGLDINAVTPEEVAVSVFAEIIKVKLREA"
     gene            complement(1090..2562)
                     /gene="lysS"
                     /locus_tag="VI541_00175"
     CDS             complement(1090..2562)
                     /gene="lysS"
                     /locus_tag="VI541_00175"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HLF69355.1"
                     /translation="MSKIPDSGEHEQARLNKLDALIAAGDEPYKLGFERTHTTGEIIE
                     KYANLEAGTETADMVRIAGRLMTLRVHGALAFSDLQDASGKIQLFAHSDEITDRFAAF
                     CDLDMGDWVGAWGRVMTTRRGELSVKIAGFELLSKCLRPLPEKWHGLKDVELRYRQRY
                     LDLITNPRAREILDARSRVVSTMRTWLEERGFIEVETPMLQTIPGGAIARPFVTHHEA
                     LGSNLYLRIAPELYLKRLVVGGVEKVFEINRNFRNEGVSTQHNPEFTMLELYEAFGDY
                     NTMAELLEGLIRTAAMAVLGTLEFEYQGRALDLASPFRRARLIDLVREAGADPDGDLQ
                     VECDKLGVGYDPDWGWGKLMLEIYDKKVEPNLFQPTFVLDFPREVSPLARQHREDPRF
                     TEHLDLVICGMEIAPAYSELTDPIDQRQRFAEQLRQREGGDVEAHLMDEEFLKALEYG
                     MPPTGGLGLGIDRLAMILTDSASIREVILFPALRPEKSER"
     gene            complement(2546..>2663)
                     /locus_tag="VI541_00180"
     CDS             complement(2546..>2663)
                     /locus_tag="VI541_00180"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719053.1"
                     /note="type III; catalyzes the formation of
                     (R)-4'-phosphopantothenate from (R)-pantothenate in
                     coenzyme A biosynthesis; type III pantothenate kinases are
                     not subject to feedback inhibition from coenzyme A and
                     have a high Km for ATP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HLF69356.1"
                     /translation="TGGLAPMILKECSTPIQHEPALTLTGLRILYERNVEDP"
BASE COUNT          481 a          830 c          835 g          517 t
ORIGIN      
        1 gtatgaggcc gagcctgaga cgaccacggc tgagggcgag gcccccgcga gttcctcgat
       61 cgctgcctca ggaccggccg acgaagccac caacgcgacg ccgcgaagag acagggcgtc
      121 agagaggatc tgcctcaccg atgttgcgcg ctgcgaggac tcaacggcaa gacccaccac
      181 tttgtagccg cgatcgtcag ggccgaagta gcgggcgaga cgccatgcct gccattcaag
      241 tgggatcact gactgaaaga ccgtcgggcc aagcgaggtt gagtagaggt catcctgcat
      301 ctcgaacacc gggaggtggt gggtctggat cgctcgcgac gcgctctgga tgatctcctc
      361 tccgccgatg gtgatggctg cgagaacatc ctcgtcgtca gagatcgcgg ccagggtcat
      421 ggcgctgcta gctgcgacct tgatcgcaag acggtagcca cggcgcatcg cagtcacctc
      481 atcggccgcg agttgagcac ccgccaaggt gtctgcgtcg gagccgagga tgtagatagt
      541 ccgcgtcggt gttggcctcg cgggggacgg atcggtctgg cgtgtgcatg aaaccagtgt
      601 cagagccagc agcccgacgg ccctaagctt ctctgagttt gaccttgatg atctcggcga
      661 agacactgac tgcgacctcc tccggcgtaa ccgcgttgat gtcgagaccc atggggatgt
      721 ggatccgctc caaggcctcg tgtgatagcc catcagctct cagggtttcc agcagctcgg
      781 gtgcatcgtc gagcatccca aggtagggga ccctggtcga cgctagcgat cgtacgacgc
      841 gcttcgaaaa ctctcggtcc tcggcgcaca ccaccacggt agtgcgctcg ctgggcctga
      901 tggcctcgac atccgtcgac tccggcgtga cgatcacttc atccgcccca tcgaactctc
      961 cgaccctcgg cgtctggggg cctgctgcca cgcgcacgtg atatcccatg ggctcgccga
     1021 tggcgacaag ggcccgcgca accggacccg tccccacgat gaggagttcc tcgatctggg
     1081 tgttcgggtt catcgctcag acttctcggg cctgagggcc gggaagagga tcacctcccg
     1141 tatcgacgcg gagtcggtga ggatcatcgc cagtcggtcg ataccgagac cgaggccccc
     1201 ggtcgggggc atgccgtact cgagggcctt caggaactcc tcgtccataa gatgagcctc
     1261 cacgtcgccc ccctcgcgct ggcgcaactg ctccgcgaat cgttgcctct ggtcgatcgg
     1321 gtccgtcaac tccgagtagg cgggtgcgat ctccatcccg cagatgacca ggtcgagatg
     1381 ctccgtgaat cgggggtcct cgcgatgctg gcgcgccagg ggtgagacct cgcgcgggaa
     1441 gtcgaggacg aatgttggct ggaataggtt gggctccacc ttcttgtcgt agatctcgag
     1501 catcagcttg ccccaacccc aatcagggtc gtagcccacg ccgagcttgt cgcattcaac
     1561 ttgcagatca ccatcggggt cagctcccgc ctcgcgcacg aggtcgatga gtcgagctct
     1621 ccgaaatggc gatgccaggt cgagagcacg gccctggtac tcgaactcca gcgtgccgag
     1681 gactgccatg gcggcggtcc tgatcaggcc ctccaacaac tcggccatgg tgttgtaatc
     1741 gccgaacgct tcgtagagtt cgagcatcgt gaactctggg ttgtgttgcg tactgacgcc
     1801 ctcgttgcgg aagttgcggt tgatctcgaa gaccttctca accccgccaa ccacgagtcg
     1861 cttgaggtat agctcgggtg cgatgcgaag gtagaggttc gagccgagag cctcgtggtg
     1921 cgtcacgaac gggcgagcga tggctccgcc cgggatggtc tgaagcatcg gagtctcgac
     1981 ctcgatgaag ccacgctctt cgagccaggt ccgcatcgtc gatacaactc gcgaccgtgc
     2041 atcgaggatc tcgcgcgccc tggggttggt gatcaggtcg agatacctct gccggtatct
     2101 cagttccacg tccttgagtc catgccactt ctcgggaaga ggtcgcagac acttgctcag
     2161 cagttcgaac ccggcgatct tgacggacag ttctccgcgc ctggtcgtca tgacccttcc
     2221 ccaggccccc acccagtcgc ccatgtccag atcacagaat gcagcgaagc gatcggtgat
     2281 ttcatcggaa tgcgcgaaga gctggatctt gccggacgca tcctgcaggt cactgaatgc
     2341 aagggcccca tgaacgcgaa gggtcatcaa gcgtccggcg atcctgacca tatccgcggt
     2401 ctcggtcccc gcttcgaggt tggcgtattt ctcgatgatc tcgcccgtcg tatgggtgcg
     2461 ttcgaagccg agtttgtacg gctcgtcgcc cgcggctatg agcgcgtcca gcttattcag
     2521 acgcgcctgc tcgtgctctc cgctatcagg gatcttcgac attgcgctcg tagaggatac
     2581 gcagtccggt caaggtcaag gcgggttcgt gctggatcgg ggtggagcac tctttgagga
     2641 tcatgggggc caggccccca gtc
//
LOCUS       DATDOZ010000013         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_272910, whole genome shotgun sequence.
ACCESSION   DATDOZ010000013 DATDOZ010000000
VERSION     DATDOZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272910"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..126
                     /locus_tag="VI541_00185"
     CDS             4..126
                     /locus_tag="VI541_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69357.1"
                     /translation="MGLYIVKNLVEEQGGLVSVSSKVAEGSTFTVRLPKRTEDR"
     gene            126..542
                     /locus_tag="VI541_00190"
     CDS             126..542
                     /locus_tag="VI541_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF69358.1"
                     /translation="MARILIVDDEPSLLKMLQVNLELEGHETLLAGDGETALKRIEAE
                     EPDLVLLDIMMPVLDGWEVLRRLELSVGRKQPRVIVMTAKAGGHDVVKGLELGADEYV
                     TKPFEMDDLLALIVVVLSRSDEAAAQRRRSMIEERS"
     gene            539..1327
                     /locus_tag="VI541_00195"
     CDS             539..1327
                     /locus_tag="VI541_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF69359.1"
                     /translation="MSDSVLLSRTGAALHITLNRPEVFNVFDDDMGRRFLEGLTEAAD
                     PSVRAVLITGAGKAFCGGEDLKALSSHYEAGEAPDLGEILLRRYNPAIEAIISLEKPV
                     IAAVNGVAAGAGFSLALACDLRVMAENSSFVLAFPKVGLVPDSGGTWLLPRYLGPGRA
                     LEVALLADPIKAARALELGLVNSVVPADELATAAADLVRRLAEGPTIAFGLSKRLIWE
                     SSQSSLVEHLTREADAQRRAGLSGDHLEGVKAFGEKRSPRFTGA"
     gene            1376..2443
                     /gene="pdhA"
                     /locus_tag="VI541_00200"
     CDS             1376..2443
                     /gene="pdhA"
                     /locus_tag="VI541_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HLF69360.1"
                     /translation="MESSQPLCVVSPDGTFDDKAASGLDLKEADLLEIYRLMSLVRRA
                     DLESTALQRQGELAVYPPLLGQEAAQVGSAYALASGDFVFPSYRELGAAIVRGIDMVE
                     YLNFYRGTWHGGTWDFNEDRFGMVSVPIASQIPHAVGYALGSKLEGKSQVTLVYFGDG
                     ATSQGDFHEGANLAAVFKAPVVLFCQNNQWAISVPVAAQTAAPIHTKAQGYGMPGVLV
                     DGNDVLATYLVTKHAIDRARSGEGPTLIEALTYRMGPHSTADDASKYQPKEQIEKWGA
                     LDPLQRYRTYLEKAGILTAPFEEQTRRDADELAAYIRQGIVGAPPPALEDLFKWVFAE
                     PTPHLSAQSAEMLSEAQGQDG"
     gene            2436..>2738
                     /locus_tag="VI541_00205"
     CDS             2436..>2738
                     /locus_tag="VI541_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HLF69361.1"
                     /translation="MAEVTMAEALNTALRHALADDEKVLVLGEDIGKLGGVFRITDRL
                     QVEFGESRVFDTPLAESGIVGVSLGLAMYGYRPVAEIQFDGFVYPAFNQIISHVAKY"
BASE COUNT          538 a          784 c          868 g          548 t
ORIGIN      
        1 ggtttgggtc tgtacatcgt taagaatctc gttgaagaac agggcggctt ggtatctgta
       61 agcagtaagg ttgcagaagg ttcgaccttc acagtgaggt tgccgaagag gacggaagat
      121 aggtaatggc gaggatactg atcgtcgatg acgaaccctc cctattgaag atgcttcagg
      181 tcaacctgga actcgagggt catgagactc tgctcgccgg agacggagag accgcactca
      241 aacggatcga agcagaagaa cccgatctcg ttctactcga catcatgatg ccggtcctcg
      301 atggatggga agtcttgaga cgtctcgagc tctcagtcgg ccgcaaacag ccgagagtca
      361 tcgtgatgac ggccaaggca ggagggcacg atgtcgtcaa agggctcgaa ctcggggccg
      421 acgaatatgt caccaaacca ttcgagatgg acgacctcct agccttgatc gtcgtggttc
      481 tctcgcgaag tgacgaagca gccgctcagc gacgccgctc gatgatcgag gagaggagtt
      541 gagcgattcg gtactgctct cgcgaacggg agcagcgctt cacatcacat tgaatcgtcc
      601 ggaggtcttc aacgtcttcg acgatgacat gggccgcagg ttcctcgagg gtctgaccga
      661 agctgccgat ccatcggttc gcgcggtgct catcacgggc gcaggcaagg cgttctgtgg
      721 tggtgaggat ctcaaggctc tctcatctca ctacgaggcg ggggaggccc ccgatcttgg
      781 cgagatactt cttcgccgat acaacccagc catcgaggcc atcatctcgc tcgaaaaacc
      841 tgtgatcgca gcggtgaacg gcgtggctgc gggggctgga ttctcgttag ctctcgcctg
      901 cgatcttcga gtgatggccg agaactcctc gttcgttctg gcattcccca aggtcggtct
      961 ggttcccgac tcaggaggca cctggctgct gccccgttat ctcggcccgg gccgtgctct
     1021 ggaggtggcc ttgctcgccg atccgatcaa ggccgcacga gctctcgagc tgggactcgt
     1081 aaactcggtg gtccctgcag atgaactggc gactgccgcg gccgatctgg ttcgtaggct
     1141 tgccgaggga ccgaccattg cgttcgggct atcgaagaga ttgatctggg agtcatccca
     1201 gtcatcactc gttgaacatc tgaccagaga ggctgatgca caacggcgtg cggggctttc
     1261 gggcgatcac ctcgaaggcg tcaaggcctt tggcgagaag cgctctccga ggttcacggg
     1321 ggcctaggct cggcctaggc tcggcccccg gtcgctcttc gatagtctta ggcatatgga
     1381 gagttcccag ccgctatgcg tcgtctcccc cgacgggacc ttcgacgata aggccgcaag
     1441 cggtctagat ctcaaagagg cggacctcct cgagatctac cgcctcatgt cactggttcg
     1501 aagggcggat ctagagtcga cggctcttca gcggcagggt gagttggctg tgtacccccc
     1561 tctgctcggc caggaggccg cccaggtcgg cagtgcctac gcccttgctt caggggactt
     1621 cgtgtttccg agttaccgcg agttgggtgc ggcgatcgtg cgtggcatcg acatggtcga
     1681 gtacctcaac ttctataggg ggacatggca cggggggacc tgggacttca acgaggatcg
     1741 gttcggcatg gtctcggttc ccatcgcaag ccagatccct catgcggttg ggtacgccct
     1801 gggctcgaag cttgagggca agtcccaggt caccctcgtc tacttcggcg acggggcgac
     1861 ctcgcagggc gacttccatg agggggcgaa tctcgcagct gtattcaagg cccccgtcgt
     1921 gttgttctgc cagaacaacc aatgggcgat ctccgtgccg gtggcggcgc agaccgccgc
     1981 tcccatccat acgaaggctc aggggtatgg gatgccgggc gtgctcgtag acgggaatga
     2041 cgttcttgct acctacctcg tgacgaagca tgccatcgac cgtgcacgct ccggagaagg
     2101 gccgacgctc atcgaagccc tgacctaccg catgggtccg cactcgaccg cagatgatgc
     2161 gagtaagtac cagccgaagg aacagatcga gaagtgggga gctcttgacc cgctgcagcg
     2221 ataccggacc tatctcgaga aggcgggcat cctgaccgcc ccattcgagg agcagacgcg
     2281 cagggatgcc gatgaactag ccgcatatat ccgccaaggg atcgtcggcg cacccccgcc
     2341 agcgctggag gatctgttca agtgggtctt tgcagagccg acaccgcacc tgtcggcaca
     2401 gagcgcggag atgttgtcgg aagcgcaggg gcaagatggc tgaggtgacg atggccgagg
     2461 ccctcaacac agcgctgcgc cacgcacttg cggatgatga aaaagtcctt gtgcttggcg
     2521 aggacatcgg caaactcggt ggagtctttc ggataacaga caggctccag gtcgagttcg
     2581 gcgaatctcg cgtgttcgac acgccactcg ccgagtcggg gatcgtcggc gtgtcgttgg
     2641 ggctcgccat gtatggatat cggccggtgg ctgagatcca gttcgatggc ttcgtctatc
     2701 cagcgttcaa ccagatcatc tcgcatgtcg ccaagtac
//
LOCUS       DATDOZ010000014         5129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2366425, whole genome shotgun sequence.
ACCESSION   DATDOZ010000014 DATDOZ010000000
VERSION     DATDOZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366425"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..810
                     /gene="rimP"
                     /locus_tag="VI541_00210"
     CDS             343..810
                     /gene="rimP"
                     /locus_tag="VI541_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HLF69362.1"
                     /translation="MRSTCEDILSRRSIELVTLDLKAGPGRQILEILVDRVDPAVPLD
                     ELTEISEEISRALDIEDPIPGRYHLEVATAGLERPLVRPSDYVRFEGRQIRLKLSEPI
                     EGRSSFKGLITSSNDETFVLQVEGGGVVEIDYGSVFRANLVVDWEKELKGGLS"
     gene            807..1877
                     /gene="nusA"
                     /locus_tag="VI541_00215"
     CDS             807..1877
                     /gene="nusA"
                     /locus_tag="VI541_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLF69363.1"
                     /translation="MNPIEMDALRLVEREKGISFESVVAALEQALAAAFKRSPDAEGD
                     EARVEIDRVTGEVKVFAQEVEEDAETGNFTVLREWEVSSAALGRIAAQTAKQVILQRI
                     RDAEREVTYGEYSGRETDIVTALVQQADPRYVLLDLGRGVEALLPQVEQVPGERYDHG
                     SRIKAYIVEVRRSPRGPQIICSRTHPNLLRRLFELEVPEIVDGIVEIKQVAREPGHRS
                     KIAVVSSDPNVDATGACVGPKGSRVRMVVNELRGEKVDIIAWSDVPAQFVANALSPAK
                     VKEVRIDEATQTAEVIVPDFQLSLAIGKEGQNARLAARLTGWRVDIKSESQILREEAK
                     KVASPPKLAEPDEAPSVVSNGA"
     gene            2152..4296
                     /gene="infB"
                     /locus_tag="VI541_00220"
     CDS             2152..4296
                     /gene="infB"
                     /locus_tag="VI541_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HLF69364.1"
                     /translation="MRVHELAKEIGLTSKEALEKLAGLGIEVKSHASSLTDDQASQLR
                     ATAVKGAPAPKSAPQRKTAPKAQAPEPKPEPAAKVPEPKPKPEPAAKAPRPVAAASEP
                     EPAAKAPEPKPEPAAKAPEPDLKVSVAAIQVTRGITVEEFAHKLERSPSEVIKILLSL
                     GEMKTITQSLSDDAVELVAGELGIEVEVVSPVSDGVDQTVVLDEEVENPANLVVRPPI
                     VTVMGHVDHGKSSILQQFRKKEMLALEAGGITQAIGAYRVHDAAGRLVTFIDTPGHEA
                     FTQMRARGAQVTDIAILVVAADDGVQPQTVEALDHARAASVPILVAVNKIDKPEADPT
                     RVRQQLAELGLQPEEWGGDTVYVDISAKQGTNLDDLLEMIHLVAEMQEFKADPAASVR
                     GVVIEAHLDKGRGPVATLIVLRGTLRVGDLVVCGTSWCRVRALTDEIGKTMPSAGPSQ
                     PVLVTGWSKVPAAGDEFRFVADERDAKRISQEREAKMRQADLVAGGKSRTLEDLLSLT
                     RAGELPELRLIVKADTQGAIEALAESIAKTDQSAVRTTLLRRAVGAINENDITLAQAS
                     GAIVVGFNVRPDQGARQLAEKEGVDVRLYEVIYQLLDDIDKATKGLLVPEEEEVVMGG
                     AQVRDLFRTPKGVVAGCYITEGKITRNSKARLLRDGTIVYTGEIASLRRFKEDVREVA
                     TGYECGIMISGYNDIKTGDVIETFEIREVARV"
     gene            4300..4599
                     /locus_tag="VI541_00225"
     CDS             4300..4599
                     /locus_tag="VI541_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HLF69365.1"
                     /translation="MNMFIGYARFDFLVPGSDSLKDKRRIVRRIVERMRARFHVSVAE
                     VDHLDLHQRSAVGASCVSNSHSHARQMLDELERFVRSEPEIEMVEVLREVVSADA"
     gene            4592..5008
                     /gene="rbfA"
                     /locus_tag="VI541_00230"
     CDS             4592..5008
                     /gene="rbfA"
                     /locus_tag="VI541_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HLF69366.1"
                     /translation="MPKNYPRSERVEQLAREVLSQAVSSLKDPRVGFATVTQVRLSPD
                     LRTARVYVSALGDDEERADTIEAIIHAAPHLRARLGEEVRLKYLPILDIVEDETAQNS
                     ERIEQLLRGVGASKPPELESPRGPDELDESYLGAED"
BASE COUNT         1048 a         1539 c         1626 g          916 t
ORIGIN      
        1 cgtgccaata cttcggggct gagtcggtca acgccttcat ctcgcagagg gtgaactaca
       61 agatcctggg ggtcgcgaaa gatctctacc tggacctgcc gctgcgtctg gaggtcaagc
      121 taccctccgc cggtacctca gcccccgcct gccccagcta ggaccaccct gagcccatct
      181 cttccggttc cgatatactg atctgacgaa aaacaggcgc cctggcgtct ttcagtgcag
      241 caaatgtggg cttgggggcc cactttttta cgcttagcga tacgacaacc taggatgagg
      301 aggaggtgag agcgattgac tcgccctgac gtcctatcct cagtgcgctc cacctgcgag
      361 gacatcctct ccaggcgttc gatcgaattg gtgaccctcg atctcaaagc cgggcccgga
      421 cggcagatcc tagagatcct ggtggatcga gtggacccag cggtgccatt ggatgagctg
      481 accgagatct ccgaggagat ctcgagggct ctcgacatcg aggaccccat cccgggccgc
      541 taccacctcg aggtcgccac ggctggtctg gagcgacccc tagtgagacc gtcggactat
      601 gtgcggttcg agggaaggca gatccgattg aagctcagcg agccgatcga gggtcgaagc
      661 agcttcaaag gtttgatcac gagtagcaac gacgagacgt tcgttctaca ggtagagggc
      721 gggggagtgg tcgagatcga ctacggctcc gtcttccgag cgaacctcgt cgttgattgg
      781 gaaaaggaac tcaaaggagg gctgtcatga atcccatcga gatggatgcc cttcggttgg
      841 tcgagcgcga gaaagggatc tcattcgaaa gtgtcgttgc ggcactcgag caggccctgg
      901 ctgccgcatt caagcgatca ccggacgccg agggcgacga agcacgagtt gagatagacc
      961 gagtaacagg cgaggtgaag gtcttcgctc aagaggtcga ggaggatgcc gagaccggca
     1021 acttcacggt ccttcgcgag tgggaggtct cgtctgctgc gctcggtcgc atcgcggctc
     1081 agaccgcaaa gcaggtgatc ctccaacgga tccgagatgc agagcgtgag gtcacctacg
     1141 gtgagtattc gggacgcgag accgacatcg ttacggccct cgttcagcag gctgacccgc
     1201 gatacgtcct tctggaccta ggtcgaggcg tcgaggctct ccttccgcag gtcgagcagg
     1261 ttccagggga gcgctacgat cacggctcgc gcatcaaggc ctacatcgtt gaggttcggc
     1321 gctcaccccg tggaccccag atcatctgct cccggacaca ccccaacctg cttcggcgac
     1381 tcttcgaact cgaggtcccc gagatagtcg atgggatagt cgagatcaag caggtcgctc
     1441 gcgagccggg tcatcgctcc aagatcgcgg tcgtttcgag cgatcccaac gtcgacgcga
     1501 caggtgcctg cgttggtcct aagggttctc gggtccggat ggttgtgaac gagctccgcg
     1561 gcgagaaggt cgacatcatc gcgtggtccg atgtgcccgc tcagttcgtc gcgaatgctc
     1621 tctcgcctgc gaaggtgaaa gaggttcgga tcgacgaagc cacccaaacg gcagaggtca
     1681 tcgttcccga cttccagctg tcgctggcca tcggaaagga ggggcagaac gcccggcttg
     1741 ctgcacgcct caccggctgg agggtcgaca tcaagagcga gtcgcagatc ttgcgcgagg
     1801 aggccaagaa ggtcgcgtct ccccctaagc tggcggaacc cgatgaagct ccttcggtgg
     1861 tgagtaacgg cgcctgagcg catgtgcgtc tggtgtcgga ccagaagaac caaggacgaa
     1921 ctgctcagat tcgttcgagt gggcgaaacg tttgttcttg acagggaagg aaggcagcag
     1981 gcccgagggg cctacacgtg caagcgacgt gagtgcgtgg agggagcgat cctctcgggc
     2041 aagatgggac gtggccttcg cgtaacggca tccccgcaag ggtcgggagt gccacttagt
     2101 gaggcgttgg agtatcttct aagtaccacg cctgaatgag aaaggtcaga tttgcgcgtt
     2161 catgagttgg cgaaggagat cgggctcacc tcgaaggagg cgctcgagaa gttggcgggc
     2221 ctcgggatag aggtcaagag ccacgcgtcc tctttgaccg acgaccaggc ctcgcagcta
     2281 cgggccaccg ccgtgaaggg ggccccggcc cccaagtcag cgccgcagcg caagacggct
     2341 ccgaaagcgc aagcccccga gccaaagccc gaacccgctg ccaaggtccc ggaaccgaaa
     2401 ccaaagcccg aacccgctgc caaggccccc aggccggtcg cggccgcctc ggagcctgag
     2461 cctgcggcca aggcccccga acccaagcct gagcccgcgg cgaaggcccc agaacccgat
     2521 ctcaaggtct cggtcgcagc gatccaggtc acccgcggca tcacggtcga agagttcgcc
     2581 cacaagctcg agcgcagccc ttcggaggtc atcaagatcc tgctctcgct cggcgagatg
     2641 aaaaccatca cgcagtcact ttcggacgat gccgtcgagt tggttgcggg agagttgggg
     2701 atcgaggtcg aggttgtctc tccggtctcc gacggtgtcg atcagacggt cgtgctggac
     2761 gaagaggtcg agaacccagc gaacctcgta gtcaggcccc cgatcgtgac cgtcatgggc
     2821 cacgtggatc atggaaagtc ttcgatcctt cagcagttcc gcaagaagga gatgctcgca
     2881 ctagaggcgg gcgggatcac tcaggccatc ggtgcgtacc gggtccacga tgctgcgggc
     2941 cggttggtca cattcatcga tacgccaggc cacgaggcgt tcacccagat gcgtgcacga
     3001 ggagcgcagg tcaccgacat cgcgatcctc gtcgtcgctg ccgacgacgg tgttcagccg
     3061 cagaccgtgg aggccctaga ccacgctcgc gctgcgagtg tccccatcct cgttgcggtc
     3121 aacaagatcg acaagcctga agccgacccg acgcgcgtgc gacagcaact ggccgagctt
     3181 ggtctgcagc ccgaagagtg gggcggcgac acggtttacg tcgacatctc tgccaagcag
     3241 ggcaccaacc tcgacgacct gctcgagatg atccatctcg tagccgagat gcaggagttc
     3301 aaggccgatc cagccgcttc ggtccgcggc gtcgtcatcg aggctcacct cgacaagggc
     3361 cgtgggcccg tcgcgacctt gatagttctg cgcggaacac tccgtgtcgg cgacctggtc
     3421 gtatgcggaa cgtcatggtg ccgggtcaga gctctcacag atgagatcgg caagaccatg
     3481 ccatcggcgg gtccttcgca gccggtgctc gtcacaggtt ggtccaaggt ccctgcggcg
     3541 ggcgacgagt tccggttcgt tgccgacgaa cgcgacgcga agcgcatctc tcaggaacgc
     3601 gaggccaaga tgcgccaggc cgatctggtt gcaggcggca agtcgcgcac gctcgaggat
     3661 cttctctcgc tgacgcgcgc cggtgagctt cccgagctgc gactcatcgt caaggccgat
     3721 acgcagggcg ccatcgaggc gctggcagag tcgatcgcta agaccgatca gagcgctgtc
     3781 cgcacaaccc tgcttcgtcg agccgttggc gcgatcaacg agaacgacat cacgctcgca
     3841 caagcatcgg gtgcgatcgt ggtcggtttc aatgtccgtc ccgatcaggg ggcccgccag
     3901 ctggccgaga aagagggcgt cgatgtccgt ctctacgagg tcatctacca actgctcgac
     3961 gacatcgaca aggccaccaa gggtctgctc gttcctgaag aagaagaagt cgtcatgggt
     4021 ggcgcgcagg tacgcgatct gttccgtact cccaaggggg tcgttgcggg ttgttacatc
     4081 accgagggca agatcacacg aaactcaaag gctcggctcc ttcgcgatgg caccatcgtc
     4141 tacaccggcg agatagcgtc ccttcgtcgt ttcaaggagg acgttcgtga ggtggcaacg
     4201 ggatacgagt gcggcatcat gatcagcgga tacaacgaca tcaagactgg agacgtcatc
     4261 gagacgttcg agatccgtga ggtcgcgcgg gtctagccga tgaacatgtt catcggctac
     4321 gccaggttcg acttcttagt acctgggtct gactccctca aggacaagag gaggatcgtg
     4381 cgccgcatcg tcgagaggat gagagctcga ttccatgttt ccgtggcaga ggtcgatcat
     4441 ctcgaccttc accagcgctc cgccgtgggg gcatcgtgtg tttcgaactc tcactcccat
     4501 gctcgccaga tgctggacga actagagcga ttcgtccgaa gcgagccgga gatagagatg
     4561 gtcgaggttc taagagaggt ggtctccgcc gatgcctaag aactatccgc gatccgagcg
     4621 ggtcgagcag ctagcgcgcg aggtgttgag ccaggccgtt tcaagcctca aagatccacg
     4681 cgtaggcttc gcaacggtga cccaggttcg gttgtcgcca gacctgcgta cggcgcgggt
     4741 ctatgtcagt gccctcggcg atgatgaaga gagggccgac accatcgaag ccatcatcca
     4801 tgcggccccc catctgcgag cgaggcttgg cgaagaggtc cgcctcaagt acctccccat
     4861 cctggacata gtcgaggacg aaaccgcgca gaactccgag aggatcgagc agctgctaag
     4921 gggcgtgggg gcctccaaac ctccagagct cgagagcccc aggggccccg atgaactcga
     4981 tgaatcctat ctaggagccg aagattgaac gctgccgatt gggtgaagat cagcgacgcg
     5041 atcgagggct catccaccgt catcgtcgca tgccatgtcg atcccgatgg agacgcgctc
     5101 ggttctatcc tggccctggc gagtctcct
//
LOCUS       DATDOZ010000015         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2106617, whole genome shotgun sequence.
ACCESSION   DATDOZ010000015 DATDOZ010000000
VERSION     DATDOZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106617"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VI541_00235"
     CDS             complement(<1..652)
                     /locus_tag="VI541_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HLF69367.1"
                     /translation="MELIRPGERSAAVLDLQSRLEDIGFELEASERGHFGPSTEKAVR
                     ALQRDRGLDVDGIVGENTWRELVESSWKLGDRLLQLSRPFSRGDDVAELQIRLNALGF
                     DAGKQDGIFGPRTARALTRFQTDLGIDPDGIAGHETIVALDRLRLVTKRGLGARTRER
                     EARRDVSRGMLDKRIVIDPGHGGTDPGSLGPSGESEAELAFRISASLARRLDSVGTL"
     gene            complement(692..1015)
                     /gene="trxA"
                     /locus_tag="VI541_00240"
     CDS             complement(692..1015)
                     /gene="trxA"
                     /locus_tag="VI541_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLF69368.1"
                     /translation="MSEIIHVTDQSFDAEVTSSAEPVIVDFWAEWCGPCRLVEPHLSA
                     IAREQAGKVRVVKLNIDENPGVASRYGVMSIPTMMLFIEGAERTRIVGARPKEAIWAE
                     VSSNL"
     gene            complement(1083..1853)
                     /locus_tag="VI541_00245"
     CDS             complement(1083..1853)
                     /locus_tag="VI541_00245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLF69369.1"
                     /translation="MARIKGALSAALALALLLGPAGSASSTPSVLSTASMRRYWAPVI
                     SNGRTFPVLLSVDGRWLNWRNTYGAPRMRLIRGTWKQVGIHEGVDIYSERSTPVVAMT
                     AGTVENIGWTFYSGYRVGVRGSDGNYYFYAHMLPDFAPGIVMGAAVQPGRRLGYLGSS
                     GYGSEPTMDEFPAHLHFGLQVGKRWVDSNETLEALFAASVANINSARSERLALDNLAS
                     AIASRFGGPASPPFEVLQAGLARVVDHGALIDQKSLLG"
     gene            complement(1829..2791)
                     /gene="trxB"
                     /locus_tag="VI541_00250"
     CDS             complement(1829..2791)
                     /gene="trxB"
                     /locus_tag="VI541_00250"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLF69370.1"
                     /translation="MQDVRDVIIIGSGPAGLTAALYTARANLHPLMIEGFEAGGQLML
                     TTEVENYPGFRDAIMGPDLMEAFREQAGRFGTEFLTDDVTEVDLTRSPFGVKVGSSEY
                     RGRAVIISTGASAKMLGLPSEKRLLGRGVSTCATCDGAFFKGRELLVVGGGDSALEEA
                     IFLTRFASKVTIVHRRDELRASKIMQDRSFANKKVSFIWNSKVVDILGTDKVEAARLE
                     DAVTGEVSEVATDGIFVAIGHTPNTKLFEGKLELDEAGYLIVGNELGSDFDPDSTYAT
                     RTSIEGVFAAGDVVDHIYRQAVTAAGMGCMAAIDAERWLESKGH"
     gene            complement(2861..3202)
                     /locus_tag="VI541_00255"
     CDS             complement(2861..3202)
                     /locus_tag="VI541_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69371.1"
                     /translation="MNSGPSDAPAYILRQTAPPFTQDPSGEPMTVKGNSFFQIVMMGA
                     SGVDLSGSTPNMTYTGPKTINANLPTLLQLVEQGDFEATLSWIFGLNHNACPTVTVLQ
                     SPLRLVLDFPA"
BASE COUNT          626 a         1059 c         1063 g          645 t
ORIGIN      
        1 tgagagtgcc gaccgagtcg agtcgccgcg ccagtgatgc cgagatccgg aaggcgagct
       61 ctgcctcgga ctcacccgac ggccccaggc tccctgggtc ggtaccaccg tggccggggt
      121 cgatcacgat gcgtttgtcc aacatcccgc gactgacatc gcgtctagcc tcacgctctc
      181 gggttctagc cccgagccca cgcttcgtta ccaaccgcag tcgatcgaga gcgacgatgg
      241 tctcatgccc cgcgatgcca tcggggtcga tgccgaggtc ggtctggaaa cgcgtcaggg
      301 cacgcgccgt tcgggggccg aagatcccat cctgcttccc agcgtcgaaa ccgagagcgt
      361 ttagccggat ctgaagctcg gccacgtcat cgccgcgcga gaacggccgg gacaactgca
      421 ggagtcgatc ccccagcttc caagagctct cgaccaactc gcgccaggtg ttttcgccga
      481 cgatcccgtc gacgtcgagc ccccgatcgc gttgtagcgc gcggaccgct ttctccgtgg
      541 aaggtccgaa atggccgcgc tcggaggcct cgagttcgaa accgatatct tcgagtctcg
      601 actgaagatc gagaaccgca gccgacctct cacccggacg gataagttcc atcggagcag
      661 attagtccgg aaagacttct aaggcaggaa gctacaggtt cgaggagacc tcggcccaga
      721 tcgcttcctt cggacgggcc ccgacgatgc gtgtgcgctc ggccccctcg atgaatagca
      781 tcatcgttgg gatcgacata acgccgtatc ggctggcaac cccggggttc tcgtcgatat
      841 tcagcttcac cactctcacc ttccccgcct gttcgcgggc gatcgcactg agatgtggct
      901 cgactagtcg acacggcccg caccactcag cccagaagtc gacgatgacc ggttcagcgg
      961 aagacgtgac ttcggcatcg aagctctgat cggttacatg gatgatctca ctcatagcat
     1021 ttcgggccgg gggccctgct cccttcgggg ttcgtcttgg taaggtgcca tatcttagcg
     1081 gctcatccga gcagtgactt ctgatcgatc aaggccccgt gatcaacgac tcgagccagc
     1141 cccgcctgga ggacctcgaa aggaggtgag gccggaccac cgaagcgcga cgcgatggcg
     1201 ctcgcgaggt tgtcgagggc gagccgctcc gaacgcgcag agttgatgtt ggccaccgac
     1261 gcggcgaaga gagcttcgag ggtctcgttc gagtcgaccc agcgtttgcc cacctggagt
     1321 ccgaagtgaa ggtgtgcggg gaactcatcc atcgtcggtt cgctgccgta ccccgaactg
     1381 ccgaggtacc ccaaccttcg gcccggctgc acggccgcac ccatgacgat cccgggcgcg
     1441 aagtcgggaa gcatgtgcgc gtagaagtag tagttgccgt cgctaccgcg tacccccacg
     1501 cggtacccgc tgtagaaggt ccagccgatg ttctcgacgg tgccggccgt catggcgaca
     1561 accggagtgg aacgctctga gtagatatcc acgccttcgt ggataccgac ctgcttccat
     1621 gttcctctga tcagtcgcat cctgggggcc ccgtaggtgt tgcgccagtt gagccaccgc
     1681 ccatcgaccg agagcaggac cgggaaggtc cgcccgttcg agataacggg ggcccagtag
     1741 cgcctcatcg atgcggtgga caagaccgat ggcgtagacg aggcagagcc ggcaggtcct
     1801 agcagcagag ccagcgcaag agccgcgctc agtgcccctt tgattcgagc catctctcag
     1861 catcgatagc ggccatgcaa cccatcccgg cggctgtcac ggcctggcgg tagatgtggt
     1921 cgacgacatc gccagccgcg aagaccccct cgatgctcgt gcgggtcgca taggtcgagt
     1981 ccgggtcgaa gtctgagccg agttcgttcc cgacgatcag gtagccagcc tcatcaagtt
     2041 ccagtttccc ctcgaagagc ttcgtgttcg gcgtgtgtcc gatcgccacg aatatgccgt
     2101 cggttgcgac ctcagagacc tcgcccgtta cagcatcttc gagccgcgcc gcctcgacct
     2161 tgtccgtgcc gagtatgtcc accactttgg agttccagat gaacgagacc ttcttattcg
     2221 cgaacgaacg gtcctgcatg atcttcgagg cacgcagctc gtcgcgccgg tgcacgatcg
     2281 tcaccttgct tgcgaagcga gtgaggaaga tcgcttcctc tagggccgag tcacccccgc
     2341 cgacgacgag gagctcgcga cccttgaaga aggcaccatc gcaggtggca catgtcgaga
     2401 cgcctcgccc gagcagacgc ttctcgctgg gaaggcccag catcttcgcc gacgctcccg
     2461 tcgagatgat gactgcccgg ccacggtatt cggatgagcc gacctttaca ccgaaaggcg
     2521 acctggtgag gtcgacctcg gtcacgtcat cggtgaggaa ctcggttccg aaccggcctg
     2581 cctgttcgcg gaacgcctcc atcaggtcgg ggcccatgat cgcgtcgcgg aaccccgggt
     2641 agttctcgac ctcggtggtg agcatcaact ggccgcctgc ctcgaagccc tcgatcatca
     2701 gagggtgaag gttcgctcta gcggtgtaga gcgcggccgt cagccccgca gggccggagc
     2761 cgatgatgat cacatctcgt acgtcttgca tctctctcag gatatatccg gacgcggaac
     2821 tgacatcgca acttcccctg ggtgtcgagc cttggtcgag tcaggccggg aagtcgagga
     2881 ccagtcggag tgggctctgc aggactgtca cggtgggaca ggcgttgtgg ttgaggccga
     2941 agatccaaga gagcgtggct tcgaagtcgc cctgttccac gagctgtagg agggtgggca
     3001 ggttcgcgtt gatcgtcttg ggtccggtgt aggtcatgtt cggggtggac ccactcaggt
     3061 caacccccga ggcccccatc atcacgatct ggaagaaaga attccctttg accgtcatcg
     3121 gctcgccgct cggatcctgc gtgaatggtg gtgcggtctg ccgaaggatg taggcgggag
     3181 catcggacgg tccactattc acagtgaact cgaagaccac gcggtcggaa tccgtctgat
     3241 gagccgtgcg gatgctgcgc agctgtagtg aggtctgcga gccccctctc tgttctgaac
     3301 attgaacagg cgcgggcgat gtgctcgggc taggtgaggc agatggagat ggtgacgggc
     3361 taggtttcgg tttgggtgcc ttggtggtgc atg
//
LOCUS       DATDOZ010000016         4043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2367409, whole genome shotgun sequence.
ACCESSION   DATDOZ010000016 DATDOZ010000000
VERSION     DATDOZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367409"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VI541_00260"
     CDS             <1..470
                     /locus_tag="VI541_00260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLF69372.1"
                     /translation="EVLFRGIIFRIMEEGIGSWGAIALSSLIFGGAHLSNPHSSVFAA
                     IAIAVEAGLMLSGAYMLTRDLWMAIGIHWSWNFFEGPVFGTAVSGGDFPVMVRSTMTG
                     PALWTGGSFGPEAGLVAFIIAGSIGGLMIYLAVKKGFTLSPMWRAKEVSPEQT"
     gene            complement(451..1326)
                     /gene="sucD"
                     /locus_tag="VI541_00265"
     CDS             complement(451..1326)
                     /gene="sucD"
                     /locus_tag="VI541_00265"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HLF69373.1"
                     /translation="MGIFLGAESRVLVQGLTGEQGKFHSLRNRGYGTKIVAGVTPGKG
                     GQTVEGIPVFNNVAEGAEQTGADVAVIYVPARSAPDAILESIDAKVPLIVCITEGIPA
                     NDMARVYSYLSDGRHCSRLIGPNCPGLISPGSSNVGIIPGEITAPGPVGMVSRSGTLT
                     YQIMHELTQLGVGQTTTVGIGGDPIVGTNFIDCLGAFEEDPETEVVVLMGEIGGDEEE
                     KAAEFISAHMKTPVVAYVAGYTAPEGRRMGHAGAIVSGSKGTAAAKAQAFERAGVRVG
                     RNSVEVAQLVKSALG"
     gene            complement(1326..2261)
                     /locus_tag="VI541_00270"
     CDS             complement(1326..2261)
                     /locus_tag="VI541_00270"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69374.1"
                     /translation="MRLFLGGSNGLVLYEDGDVTSLHQEPVLCALRTSSSRLVAGTAS
                     GWTLVWEGNGDSRVSAKDLGDAVHSLAQTANNVLFAGSMPANTWVSKDGGDSWKLSPA
                     LAEAPNYESWTAPWGIPVAASIAAHPKDAKTLYVGIEQGGIYRTRDAGRRWFDLEIPG
                     SDVHSIQICPANHLRIYVTTGEGSFCSDDEGFNWRLMGPTNARQYTMGLAAHPTEADR
                     VIISAAEGPPPAWGKSGARCDIYLSTDAGRRFRTVAKGLKGGVGRKALVINSKVPSEI
                     VFGTSLGELYYSNDGGESFDLEAKGLGDLNAIAFA"
     gene            complement(2258..3400)
                     /gene="sucC"
                     /locus_tag="VI541_00275"
     CDS             complement(2258..3400)
                     /gene="sucC"
                     /locus_tag="VI541_00275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HLF69375.1"
                     /translation="MDLFEYQGKELLARWGIPVPEGGVATTPREAEKIAESLGGSVVV
                     KAQVQVGGRGKAGGIKVVSSPEAALEAASAILGMDIKGHKVERLLVEKASAIKQEYYL
                     SVLLDRSQKQFLLMLSSKGGMDIEEVAATDPSALARVHVDALLGLRSFHINQLVYGSG
                     IDPAACKPTAGIIAKVFLLFIEADATLVEINPLILTSDGSVVALDAKVSLDDSASFRH
                     PEFEELREVSAADPFERMAKERGFGNFIKLDGNVGIIGNGAGLVMSTLDVVDQCGGRA
                     ANFLDVGGGADAEALSSALEVILADPAVRSVLINIFGGITRCDLVAEGVVDALSRLEV
                     RVPIVVRLDGTNAEQGRRILAEARNPNVIPAETMSQAAERAVEAAR"
     gene            complement(3448..3861)
                     /locus_tag="VI541_00280"
     CDS             complement(3448..3861)
                     /locus_tag="VI541_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HLF69376.1"
                     /translation="MNDGHRIRVVVAKPGLDGHDRGIKVVARALRDAGVEVIYTGLHQ
                     TPEQIAEAAIQEDADAVGLSCLSGAHMTLFPKVVDILRSRGAADVQVFGGGIIPSDDV
                     PKLKEAGIVEIFTPGASTQQIVEWVQEHCGRAQKV"
BASE COUNT          812 a         1281 c         1164 g          786 t
ORIGIN      
        1 aggaggtcct cttccgaggg atcatcttcc gcatcatgga agagggcatc ggatcgtggg
       61 gagcgatcgc actcagctcg ctcatcttcg gtggggccca ccttagcaac ccgcactcga
      121 gcgtcttcgc ggctatcgcc atcgcggtcg aggcggggct gatgctcagc ggcgcctaca
      181 tgctcactcg ggatctatgg atggccatcg gcatccactg gtcatggaac ttcttcgagg
      241 ggcctgtatt cggaacagcg gtttcaggtg gtgacttccc tgtcatggtg agatccacga
      301 tgaccggccc tgccctctgg accggcgggt cgttcggacc cgaagccgga ctggtcgcat
      361 tcatcatcgc agggtcgatc ggagggttga tgatctacct tgctgtgaag aagggcttca
      421 ctttaagccc gatgtggagg gcgaaagaag ttagcccaga gcagacttga cgagttgggc
      481 cacctcgacg gagttccgac caacgcgaac gccggccctc tcgaacgcct gggccttagc
      541 tgcagccgtt ccttttgaac ccgagactat cgcgccagca tgccccatcc gacggccctc
      601 gggagctgtg tatcccgcga catacgcgac gacgggggtc ttcatgtggg cagagatgaa
      661 ctcggcggcc ttctcctctt catcgccacc gatctcgccc atgagaacga cgacctcggt
      721 ctcgggatct tcctcgaatg cccccagaca gtcgatgaag tttgttccga cgatggggtc
      781 cccaccgatg ccgacggtag tcgtctgtcc gacgccgagc tgtgtgagtt catgcatgat
      841 ctggtaggtc aaggtccccg atcgcgacac catcccaacg ggtccgggag cggtaatctc
      901 tcccgggatg atcccgacgt tgctgctccc cggagagatg agacccggac agttaggccc
      961 tatcagacgg ctgcagtgac gaccgtccga gagatacgag tagacccgag ccatgtcgtt
     1021 ggctggtatc ccttcggtga tgcagacgat gaggggaacc ttcgcatcga tcgattcgag
     1081 tatcgcgtcg ggagcggatc tcgccggaac gtagatcacg gccacatcgg caccggtctg
     1141 ctcagcacct tccgcaacgt tgttaaagac cgggatgcct tcgacggtct gaccaccctt
     1201 accgggagtg acgccagcaa cgatcttcgt gccgtatccg cggttgcgaa gcgagtgaaa
     1261 cttgccctgc tcgccggtga gcccctgcac cagaacccgc gactcggctc ctaggaagat
     1321 ccccatcagg cgaatgcgat cgcgttcaga tcgccaaggc ccttggcctc gaggtcgaag
     1381 gactctccgc cgtcgttgga gtagtagagc tcaccgagcg acgtgccgaa gacgatctcc
     1441 gacggaacct tcgagttgat gaccagggcc ttgcgaccta cgccaccctt gagccccttc
     1501 gcgaccgtgc ggaaacgtcg gcccgcatcg gtcgagaggt agatgtcgca tctggccccc
     1561 gacttccccc atgcaggagg cggaccctcc gcagccgaga tgatcactcg gtcagcctca
     1621 gtcgggtggg ccgcgagacc catcgtgtac tgcctggcgt tcgtcggacc catgagccgc
     1681 caattgaaac cttcatcatc cgaacagaaa gaaccttcgc cggtggtgac atagatcctc
     1741 agatgattag cggggcagat ctggatcgag tggacatcgc ttccggggat ctcgaggtcg
     1801 aaccagcgac gacccgcgtc acgtgtcctg tagataccgc cctgctcgat gccgacgtag
     1861 agagtcttgg cgtccttggg atgcgccgcg atcgacgcgg cgaccggtat cccccaaggc
     1921 gccgtccagg actcgtagtt gggggcctca gcaagtgcgg gcgatagctt ccagctatct
     1981 cctccgtcct tactgaccca ggtattcgct ggcatcgacc cagcgaagag gacattgttt
     2041 gcggtctgag ccaacgagtg aacagcgtct cccagatcct tcgcgctaac gcgcgagtcg
     2101 ccattgcctt cccagaccag ggtccacccc gaagcggtcc ctgcgacaag cctagaagag
     2161 ctcgtgcgaa gagcacatag aacgggctct tgatgcagcg atgtaacgtc gccgtcttca
     2221 taaaggacca agccgttgga ccctcccagg aataatctca tcgtgcagcc tccacagctc
     2281 tctcggccgc ctgcgacatc gtctcggcgg ggatgacatt ggggtttctc gcctccgcca
     2341 ggatcctgcg accctgctca gcgttggttc cgtcgagcct tactacgatg ggtacccgta
     2401 cttccaggcg gctgagtgca tcaacgacac cctcagctac gaggtcacat cttgtgatcc
     2461 caccgaatat gttgatcagg accgagcgca ccgccggatc ggcaaggatg acctcgagag
     2521 ccgatgagag ggcttcggca tccgcgcccc cgccgacatc caagaagttg gcggcgcggc
     2581 ccccacactg gtcgaccaca tcgagggtcg acatgacaag tcccgcaccg ttgccgatga
     2641 tgccgacgtt gccatcgagt ttgatgaagt tcccaaagcc gcgttccttg gccatgcgtt
     2701 cgaaaggatc ggctgctgag acctctctca actcctcgaa ctcggggtgg cggaacgaag
     2761 cagaatcatc gagcgagacc ttggcgtcga gagcaacgac ggaaccgtct gacgtaagta
     2821 tcaaggggtt gatctcgacg agcgttgcat cggcctcgat gaacagcaga aagaccttcg
     2881 cgatgatccc tgcggttggt ttgcaagcag caggatcgat gccggacccg tagaccagtt
     2941 ggttgatatg gaatgaccta agtcccaaaa gagcatcgac atgaactctt gccagcgccg
     3001 aggggtcggt cgccgcaacc tcttcgatgt ccatccctcc tttggaggac agcatcagca
     3061 agaactgctt ctgggaccta tcgagaagaa cggagaggta gtactcctgc ttgatggcag
     3121 aagccttctc gaccaggagt cgttccacct tgtgaccctt gatgtccatg cccaggatcg
     3181 cagatgcagc ctcgagcgcc gcctctggcg agctgacaac cttgatgccg ccggccttcc
     3241 cgcggccccc aacctggacc tgagccttca cgacaaccga gccccctaag gactctgcga
     3301 tcttctcagc ctcacgaggg gtggtcgcga ccccgccctc gggcacgggg ataccccatc
     3361 gggcaagcaa ctccttgccc tgatattcga atagatccaa aactggttct ccgtgacatc
     3421 gttgatacgc cgttgagatt cagacggtca gaccttctgt gcgcgaccgc aatgttcctg
     3481 cacccactcg acgatctgtt gagtcgatgc cccaggagtg aagatctcga cgatccccgc
     3541 ctccttgagc ttgggtacgt catcggaagg gatgatccct ccgccaaaca cctgcacatc
     3601 cgccgccccc cggctgcgaa ggatatcgac aaccttgggg aacagagtca tgtgggcacc
     3661 cgacaagcat gagagaccca ccgcatcggc atcttcttgg atggcggcct cagcgatctg
     3721 ctccggggtc tgatggagtc ccgtatagat cacttcgaca cccgcatccc gcagggcccg
     3781 cgcgacgacc ttgatcccgc gatcgtgacc gtcgaggccg ggtttggcca cgacaacacg
     3841 gatacgatgt ccgtcgttca cactctcatc caagggctcg ggaagtccga aaagttgcgc
     3901 agatacgccg cataaatgta gaccaaaacc cctccgcttg ctcaggagac cctggtgtcg
     3961 ctcatagacg aacgcatatc ccgatacttc gatgccttcg aggcgttcag cccagcgctg
     4021 cggatgcccg accccatcgg ctt
//
LOCUS       DATDOZ010000017         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2368133, whole genome shotgun sequence.
ACCESSION   DATDOZ010000017 DATDOZ010000000
VERSION     DATDOZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368133"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VI541_00285"
     CDS             <1..787
                     /locus_tag="VI541_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF69377.1"
                     /translation="MKINPIAAKGFGIGTGDYERARPDYPADAVAHLIGALELGRETI
                     LLDLAAGTGKLTNQLTEHVSTTIAVEPLDSMRAVLSRTVPRVQSLAGTAEAIPLDDQS
                     VDAVTVGQAFHWFDAPRAIAEIHRVLKSGGGLGLIWNLLDTRRRWVLRAEELTEPKRK
                     GVGRMLDSGWRSHFDRTSAFEPLVERWFEHRQLVDRETVLARYRSKSFIAAMDPPDQE
                     DLMGAIGGLLDGDPETRDRELIEIPYRTVVFCTRAVSADSWRG"
     gene            802..885
                     /locus_tag="VI541_00290"
     tRNA            802..885
                     /locus_tag="VI541_00290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:836..838,aa:Leu,seq:caa)
     gene            complement(886..1659)
                     /gene="trpA"
                     /locus_tag="VI541_00295"
     CDS             complement(886..1659)
                     /gene="trpA"
                     /locus_tag="VI541_00295"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HLF69378.1"
                     /translation="MIPLQERLIGLRDGGRKLFVPYVTAGLPSEVEFAALLGGLAEHA
                     DAIEVGIPFSDPMMDGPVIQESSRRAIEVGVTARSTFELLATLDVPVPVVVMTYVNIV
                     DAFGADEFISAMTACAVQGAIIPDLPLEASGRLCADMADAGLGLVQMVAPSTSAGRAG
                     QIAHSSRGFVYAVSRMGITGLRTSLSDEAKAVVEKIRPHTSLPVLLGIGISDGPQARE
                     AASIADGVVVGSALVRRVLDGDVAGALTLAGEIRAALER"
     gene            complement(1656..>2358)
                     /locus_tag="VI541_00300"
     CDS             complement(1656..>2358)
                     /locus_tag="VI541_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLF69379.1"
                     /translation="SGTLTLKDAVNEAFRDWVTNVETTHYVIGSVVGPHPFPELVARL
                     QSVIGLEARAQSLELLGRLPDAVVACVGGGSNAIGMFRAFIEDSSVALWGVEAAGEGL
                     EGHHAATLTRGRPGIFHGARSYVLQDEFGQIEEAHSISAGLDYPGVGPEHSWLKESGR
                     ASYLSATDGEALEAFQLLSRTEGILPALESSHAIARAVDIARELGPQAELLVCLSGRG
                     DKDVEIVARAIGMSP"
BASE COUNT          486 a          751 c          705 g          416 t
ORIGIN      
        1 gatgaagatc aaccctatcg ctgccaaagg tttcggtatt ggtaccggcg attacgagag
       61 ggcgcgtccg gactatccag ctgatgcggt agcgcatctc atcggcgcgc ttgaactcgg
      121 cagggaaacg atcctgctcg atctcgccgc cggcacgggg aaactaacga accaattgac
      181 ggagcacgtc tcgacgacga tcgccgtcga acctctcgac tcgatgcggg cggtattgtc
      241 ccgcacggtc ccccgtgtcc aatcgctcgc gggaacagcc gaggcgatcc ccctcgacga
      301 ccagagtgtc gatgcagtca ccgttggtca ggccttccat tggttcgacg ctccccgagc
      361 catcgcagag atacaccgag tcctgaaatc cggtggcgga ctcggtctta tctggaatct
      421 gctcgatacg cggcgccgat gggtccttcg ggcagaggaa ttgaccgagc ccaagcgaaa
      481 gggtgtgggg cggatgcttg actctggatg gaggtctcac ttcgaccgga cttccgcgtt
      541 cgagccattg gtcgagagat ggttcgaaca tcggcagttg gttgataggg agacagtact
      601 agcccgatac cgctcgaaga gcttcatcgc ggcgatggat ccgccggacc aggaggatct
      661 gatgggggcc atcggtggcc tcctagatgg agaccccgaa acacgtgacc gcgagctgat
      721 cgagatcccc taccgcaccg tcgtcttctg cacacgggcg gtctctgcgg actcgtggcg
      781 cggttagact tcaagtcgaa cgccgggatg atgaaatcgg caaacatgat cgtctcaaaa
      841 gcgattgagc taacgctctt gcgggttcga gtcccgctcc cggcactatc gttcgagggc
      901 tgcgcgaatc tcaccggcta gtgtgagggc ccccgcgaca tcgccatcta gaacccttcg
      961 gaccagggct gaaccgacga cgactccgtc tgcgatcgat gcagcttcgc gcgcttgcgg
     1021 accatcggat atcccgatgc ccagaaggac cgggagcgac gtgtggggcc tgatcttctc
     1081 caccaccgct ttggcttcat cggataacga cgtgcgaagc cccgtgatac ccatacgcga
     1141 cactgcatag acgaacccac gcgacgagtg cgctatctgg ccagcccgac ctgccgaggt
     1201 cgagggtgca accatctgta cgagaccgag acccgcatcc gccatgtccg cgcagagccg
     1261 accggaggct tcgagaggca ggtcggggat gatcgcaccc tgaacggcgc atgcggtcat
     1321 ggccgagatg aactcatccg caccgaacgc atcgacgatg ttgacgtagg tcataacgac
     1381 cacggggacc ggcacgtcaa gggtcgcaag caactcgaac gtcgaccggg ccgtcactcc
     1441 cacctcgatc gctcggcggg atgactcctg gatcacagga ccatccatca tcgggtccga
     1501 gaatgggatc ccgacctcga tggcatcggc atgctcggca aggcccccaa ggagcgccgc
     1561 gaactccacc tctgaaggca gaccagccgt cacatacggc acgaagagtt tgcggccccc
     1621 atcacgcaga ccgatgaggc gttcctgaag tgggatcacg gggacatccc aatggctcgg
     1681 gcaacgatct cgacatcctt gtcgcccctt ccagaaagac agacgagaag ttcggcttgg
     1741 gggccgagct cacgcgcgat atctacggct cttgcgatcg catgagacga ctcgagggcc
     1801 ggaaggatcc cctcggtccg gctcaaaagt tgaaacgctt caagggcttc cccatccgta
     1861 gcactcaggt atgaggctcg ccctgactcc ttgagccacg agtgttcggg cccaacacct
     1921 ggatagtcga ggcccgccga gatcgagtgc gcctcctcga tctgaccgaa ctcatcctga
     1981 agtacatatg agcgtgcccc atggaagatg ccaggtctcc cccgcgtgag ggtcgcggcg
     2041 tgatgaccct cgagcccctc gcctgcggcc tcgactcccc agagagcaac cgatgaatcc
     2101 tcgatgaagg cacggaacat cccgatcgca ttcgaccctc ccccgacgca tgcgaccacc
     2161 gcatcgggca accttcccag taactcaagg ctctgtgctc gcgcctcgag accgatgacc
     2221 gattgcagac gtgcgaccag ctccgggaac gggtggggac ccacgaccga cccgatcacg
     2281 tagtgggtgg tttcgacgtt ggtcacccag tcgcggaatg cctcgttgac cgcgtccttt
     2341 agggtgagcg taccactg
//
LOCUS       DATDOZ010000018         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_799157, whole genome shotgun sequence.
ACCESSION   DATDOZ010000018 DATDOZ010000000
VERSION     DATDOZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799157"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VI541_00305"
     CDS             complement(<1..133)
                     /locus_tag="VI541_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLF69380.1"
                     /translation="MIDFAADGAEAPGYLAAHGKGPGLIVIQEWWGLVDHIKDVCDRF
                     "
     gene            complement(175..906)
                     /locus_tag="VI541_00310"
     CDS             complement(175..906)
                     /locus_tag="VI541_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLF69381.1"
                     /translation="MNIAVKGDWGAGTQAQRDVTAAMCRERKRRNFAVVITTGDNFYR
                     PDGRATDKNFSDPERCLIDGGVTWRAVWGNHDLAGSSTKDVLGAERRYTWSEGDVGFF
                     MLDSNNVDADQTRWLETQLKRSTARVKIAAFHHPVFTDGPHEDTAAIKASWVPLFERW
                     GVALVLTGHNHDYEHAVSGGVTYVVTGGGGAQVYPCLRREAEVLRCLPENHFLLLTFT
                     ASSVTVEAIGVDGKRLDRFKVPISR"
     gene            complement(1010..1471)
                     /locus_tag="VI541_00315"
     CDS             complement(1010..1471)
                     /locus_tag="VI541_00315"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1343..1345),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLF69382.1"
                     /translation="MEEGSVAPDFSLIDQDGQTFTLSDHLGRWLLLWWYPKAATPGUA
                     LEGQGFRDRIPLFEALNATVVGASFDEPSANKEFALAYDFPFRLLSDETKEVADAFGV
                     SFPLEHEYREYAKRLSFLIDPEGRINRVYVVRDLNAHPDEVLADIERAKVS"
     gene            1511..2542
                     /locus_tag="VI541_00320"
     CDS             1511..2542
                     /locus_tag="VI541_00320"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HLF69383.1"
                     /translation="MTLPFDPPLLPMLAKAAAKIPVGDGWRYEPKWDGFRAIVFRDGD
                     SLHIGSRNAKPLERYFPELVHTLRESLADPAIVDGEIVIAGRHGLDFDALQLRIHPAS
                     SRVNMLAQQMPSTFVAFDLLADDEGDHRDDPLDERRGRLIASVQPSENSFLTPQTSDA
                     AQAEDWFHMFEGAGLDGVVAKRQDGAYTPGQRTMIKVKHKRTADCVVAGYRPSVVPDA
                     IGSLLLGIYREDDLLYVGHTSSFKDEERVAMFGLLQGFVTDESFGQSRAPGGPSRWSG
                     AQEKPWVALRPELVCEVTFDHLQGDRFRHGTTFLRWRPDKSPHECTWDQLIPPNPFDL
                     DEIRKMSSA"
     gene            2539..2964
                     /locus_tag="VI541_00325"
     CDS             2539..2964
                     /locus_tag="VI541_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006769433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69384.1"
                     /translation="MSARFDVLFVCVHNAGRSRMAEALFNRIAAGRFTARSAGTEPAA
                     HPHPEVVAAMREIGIELEGGPGTLLTQELASDAGRVIGMGCDVEAACPALRVPLEDWE
                     LPDPRGQGVDEVAAIRDEIASRVTSLLDEMAASALEDQP"
     gene            complement(3092..>3293)
                     /locus_tag="VI541_00330"
     CDS             complement(3092..>3293)
                     /locus_tag="VI541_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69385.1"
                     /translation="DEGDQMRRVRRAVFVIVFALAGLVPVTAALALPGCVGIVDHNCT
                     IEGLNCRTYVVRPNGGYLCSIM"
BASE COUNT          664 a         1029 c          964 g          636 t
ORIGIN      
        1 cgaaacggtc acaaacatcc ttgatgtggt cgaccagacc ccaccactcc tggatcacga
       61 tgaggccggg acccttgccg tgcgcagcca ggtaaccagg cgcctcggca ccatcggctg
      121 cgaaatcgat catctgtccc acgctctacc tcctcgactt tgataggtca gagactatcg
      181 cgagatgggg accttgaagc ggtcgagacg cttgccatcg acacctatcg cttcgacggt
      241 cacagagctc gccgtgaaag tgagcaacaa gaagtggttc tcaggaaggc agcgaaggac
      301 ctccgcctcg cggcgaagac acggatagac ctgagctcca ccccctccag tcacgacgta
      361 tgtaacgccg cctgagactg cgtgctcgta atcatggttg tggccggtta ggacgagagc
      421 tacgccccag cgctcgaaga ggggaaccca gctcgccttg atggcggctg tgtcctcgtg
      481 aggaccatcc gtgaagaccg gatggtggaa ggccgcgatc ttgacccgag cggtcgatcg
      541 tttcagctgt gtttctagcc aacgcgtttg gtccgcgtcg acgttgttcg agtcgagcat
      601 gaagaatccg acatcacctt cggaccaggt gtatcggcgt tccgcgccaa ggacatcctt
      661 ggtcgaggaa cctgcgaggt cgtggttacc ccagacggct cgccaggtaa cccctccgtc
      721 gattagacat ctctccgggt ccgaaaaatt cttgtcggtc gcgcggccat ccggacggta
      781 gaagttgtct ccggttgtga tgacaactgc gaagttgcgg cgcttgcgtt cgcggcacat
      841 cgccgcggtc acgtcgcgtt gcgcctgcgt cccggccccc caatcgccct tgacggcgat
      901 gttcaactct cgtctgggtg aaggcgtcgc tttcggttga gatggctggg cgcaggcgct
      961 caacgaaatc gagcagatca gcaagagcct gaagaacctc atggttctac taggaaacct
     1021 tggccctctc gatgtcggcc agaacctcat cgggatgtgc attgagatcc ctgacgacgt
     1081 agacgcggtt gatgcgaccc tccgggtcga tcaagaagga gagtctcttg gcgtactccc
     1141 ggtattcatg ctccaacggg aaagagactc cgaatgcgtc agccacctct ttcgtttcgt
     1201 cagaaagcaa ccggaatgga aagtcatagg ccaacgcgaa ctccttgttg gcggatggct
     1261 catcgaatga agcacccacg acggtggcgt tcagggcttc gaacaacggg attcgatcac
     1321 ggaacccctg accttcgaga gctcagccag gcgtcgccgc cttcggatac caccagagca
     1381 acagccaccg acccagatga tccgagagcg tgaaggtctg cccgtcttga tcgatcaagc
     1441 tgaagtcggg ggcgaccgat ccttcttcca ccaagtcccc cctgaactct tatgagcgta
     1501 aatttacccc atgacgcttc ctttcgaccc acccctgctc ccgatgctcg ccaaggcggc
     1561 cgcgaagatt cctgtcggcg atggttggag atacgaacca aagtgggatg gcttcagggc
     1621 catcgtcttt cgcgatggcg actcgctaca catcgggagc cgaaacgcga agccgttgga
     1681 gaggtacttc cctgaactgg ttcacaccct gcgggaatct ctcgcagacc cagccatcgt
     1741 cgacggcgag atcgtgatcg ctgggcgcca cgggctcgac ttcgacgctc tgcaacttcg
     1801 gatccaccct gcaagctcgc gagtgaacat gctcgcgcag cagatgccct cgacattcgt
     1861 cgcgttcgac ctcctcgcag acgatgaagg cgaccacagg gatgaccctc tcgacgagcg
     1921 caggggccgt ctcatcgcat cggttcaacc atccgagaac tctttcctca ctccacagac
     1981 gagcgacgcc gcgcaggctg aagactggtt ccacatgttc gagggcgcag gtttggacgg
     2041 ggtcgttgca aagcgccaag acggcgcgta cacgccgggg cagcgcacga tgatcaaggt
     2101 caagcacaaa cgcacggccg actgcgtcgt cgctggttat cgcccctcgg tggtccccga
     2161 cgcgatcggc tcactccttc tcggtatcta ccgcgaagac gaccttctct atgtgggtca
     2221 cacgtcctca ttcaaggatg aagagagggt ggcgatgttc ggcctcctcc agggcttcgt
     2281 aaccgacgag agtttcggtc aaagtcgcgc ccctgggggg ccgagccgct ggtcgggggc
     2341 ccaggagaaa ccatgggtcg cgcttcggcc cgagctcgtc tgcgaggtga ccttcgatca
     2401 tctccagggc gatcgtttcc ggcatggcac cacctttctt cgatggcgtc ctgacaagtc
     2461 tccgcacgag tgcacatggg atcagctgat cccaccaaat ccattcgatc tcgatgagat
     2521 ccggaagatg agttcggcgt gagcgcgcgt ttcgacgttc tgttcgtctg tgtccacaac
     2581 gccggccgct ctcgcatggc ggaggcactc ttcaacagga tcgcggcggg ccgcttcacc
     2641 gcgcggtccg ctggcacaga gcccgcagcg catcctcatc cagaagtcgt ggccgcgatg
     2701 cgggagatcg gcatcgaact cgaaggtgga cctggaacgc tcctgaccca ggagttggca
     2761 tcggatgcag gccgcgttat cggcatgggt tgcgacgtgg aagcagcatg tcctgcgctg
     2821 cgcgttccgc tggaagattg ggagctgccc gaccctcggg gccaaggggt cgacgaggtt
     2881 gctgcgatcc gcgacgagat cgcttcccgc gtaacatcgc tcctagatga gatggctgcc
     2941 tccgccctgg aggaccagcc ctaacggccc tgctagccac cccgaggtac gcaagcacgt
     3001 cgatgagggg atgcatctcc aactcattcc cacttgggct acaccagaag aagccgatgc
     3061 ggccggggtc gtaaaggctc tcgggatgat ctcacatgat cgagcacaga tatccgccgt
     3121 taggcctcac aacgtaggta cggcaattga ggccttctat ggtgcagttg tgatccacta
     3181 tcccaacgca cccgggcaat gctagagctg cagtcaccgg aaccagtccg gccagcgcga
     3241 acacaatcac gaacacagcc ctgcgcaccc ttctcatctg gtctccttcg tct
//
LOCUS       DATDOZ010000019         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1063361, whole genome shotgun sequence.
ACCESSION   DATDOZ010000019 DATDOZ010000000
VERSION     DATDOZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063361"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VI541_00335"
     CDS             complement(<1..325)
                     /locus_tag="VI541_00335"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HLF69386.1"
                     /translation="MTRVTQSDLIDRLISTRGTVMLLGGLDAGKTSFGLSLAQEAKRQ
                     GVEVGYVDADISQSTIGPPTCIGLKFTSRLEKVDLESVRQADELGFVGSTTPESHLLP
                     HVVGTA"
     gene            complement(419..610)
                     /locus_tag="VI541_00340"
     CDS             complement(419..610)
                     /locus_tag="VI541_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF69387.1"
                     /translation="MSRETISEFLTVNEVARLMRVSNMTVYRLIKSGSLRAVQVGNRY
                     RVRESDVHQYLDDHYVRAV"
     gene            706..1470
                     /locus_tag="VI541_00345"
     CDS             706..1470
                     /locus_tag="VI541_00345"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLF69388.1"
                     /translation="MSKIELSLEGKVALVTGASRGIGRAIAVAFGAHGAAVSVSARNV
                     EDLEQTAKLVEEAGGRALVVPCDVTQTDQVEAMVHQTISGLGGLEIIVNNAGGTRFMS
                     PLAAMREDGWNKVIDLNLTSVFRVCKAVAPHLIEQKKGSVINISSIDGVEPTPLRANY
                     SAAKAGVIALTRVLSQEWAELGIRVNTISPGAVSTEIWGSLIEDEHFVKMVTDRIPMK
                     RWATPEEIASTAVYLASDAASYVTGANFIVDGGSTA"
     gene            1511..1972
                     /locus_tag="VI541_00350"
     CDS             1511..1972
                     /locus_tag="VI541_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69389.1"
                     /translation="MRRFLALMLFAAAFIAAPAPATTTTQTKPYNIATGNTADCGGFD
                     DVCFTIPTSTSHVTITVNDSVSLTGVGGRYFFYKGRDCYPDGYGGHICYRGDVIAAST
                     GSFCTTVPSVVIPADAYELEVWVSALILDPPCGGIPRPGISGSVKAEFSGP"
     gene            complement(1969..>2331)
                     /locus_tag="VI541_00355"
     CDS             complement(1969..>2331)
                     /locus_tag="VI541_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF69390.1"
                     /translation="MLPTMRIAQEEIFGPVTGIMSFETLDEAIAIANSTRYGLSCSIY
                     TRDITATFKAITELEFGVVYINAPTIGAEVQLPFGGMKSTGNGHREAGPQSLDEFMEW
                     KAVSVDFSGRVQKAQMKD"
BASE COUNT          465 a          731 c          698 g          437 t
ORIGIN      
        1 gggccgtccc caccacatgg ggcagcaggt gactctctgg ggtggtggaa ccgacgaaac
       61 ccagttcatc agcctgcctc accgattcga gatcgacctt ctccaaacgg gaggtgaact
      121 tgagccctat acaggtgggg gggcctatgg ttgattggct gatgtcggca tccacgtatc
      181 cgacctctac cccctggcgc ttggcctcct gcgcaaggct caggccgaaa ctggtcttgc
      241 ccgcgtcgag gccccccaga agcatcacgg tgccgcgcgt cgaaatgagt ctgtcgatga
      301 gatcgctctg cgttactcgc gtcatgtcgc ctccctgaga caggggggga tgcctcgata
      361 catgggtcag acctacgcgg ccccttggca agggcgcggc tctcgagcca acgccttcct
      421 agacggctcg gacgtagtga tcgtccagat actggtgtac atcagactcc cggacccggt
      481 aacggttgcc cacctgcacg gcccgaaggg agcccgactt gatcagccgg tagacagtca
      541 tgtttgacac acgcatgaga cgagcgacct cgttcacggt cagaaattca gatatcgttt
      601 ctcgagacaa cgtcgcttca cccctttcac agaatcatac tacggccaca tgagtcacgg
      661 gggcctcaga agggaccccc caacgaccgg gaaggagccg ctcagatgtc caagatcgaa
      721 ctatcgctcg aagggaaggt tgccctagta actggagcca gccgagggat cggccgggcc
      781 atcgccgtgg ctttcggtgc tcatggagcg gcggtatccg tctcggctcg caatgtcgag
      841 gacctagagc agactgccaa gctggtcgag gaggccggag gcagggccct cgttgtcccg
      901 tgcgacgtga cccagaccga ccaggtcgag gcgatggtgc atcagaccat ctcggggctc
      961 gggggcctcg agatcatcgt caacaatgcc ggcggaactc gcttcatgtc tcccctcgcc
     1021 gccatgcggg aggacggctg gaacaaggtc atcgacctca acctgacctc ggtcttccga
     1081 gtctgcaagg cggtggcgcc ccatctgatc gagcagaaga aggggtccgt gatcaacatc
     1141 tcctcgatcg acggagtcga accgacgcca ctgcgagcga actactcagc cgccaaagct
     1201 ggcgtgatag cgctcacgcg ggtcctctct caggagtggg ccgagttggg aataagggtc
     1261 aacaccatct caccgggggc cgtatctacc gagatctggg gctccctcat cgaggacgag
     1321 cacttcgtca agatggtcac cgaccggatc ccgatgaaac gctgggctac accagaagag
     1381 atcgcgagca ctgcggtcta cctcgcctca gacgccgcct cctacgtcac tggggctaat
     1441 ttcatcgtcg atgggggttc aaccgcctag aggggcagga atcgggggcc cgccgtcgaa
     1501 tcaacatagg atgcgaaggt tcctggcatt gatgctgttc gccgccgcct tcatcgccgc
     1561 gcccgcgcca gcaaccacga cgacccaaac caagccatac aacatcgcga ctggcaatac
     1621 cgcagactgc ggcgggttcg acgatgtctg cttcacgatc ccaaccagca cttctcacgt
     1681 gacgatcacg gtgaacgact ccgtgtcgtt aacgggagtc ggcggacgct acttcttcta
     1741 taagggacgc gactgctacc ccgacggata tggagggcac atctgttaca gaggtgacgt
     1801 gatagccgcg tccaccggat ctttctgcac cacggttccc tcggtggtca tacccgcgga
     1861 tgcctatgaa ctcgaagtct gggtctcggc gctcatcctt gacccgccat gtggcggcat
     1921 ccccaggccg gggatatccg gaagcgtcaa ggctgagttc tcgggccctt agtccttcat
     1981 ctgtgccttc tgaacccgcc ccgagaagtc gaccgatacc gccttccact ccatgaactc
     2041 gtcgagcgat tgggggcccg cctcgcggtg accgttgccg gtcgacttca tgcccccgaa
     2101 cggaagctgc acttcagcgc cgatggtcgg agcattgata tagacgacac cgaactcgag
     2161 ttcggtgatc gccttgaagg tcgccgtgat atcgcgggtg tagatggagc atgacagccc
     2221 gtagcgcgtc gagttcgcga tcgcgatcgc ctcgtcgagg gtctcgaatg acatgatgcc
     2281 cgtcaccggg ccgaagatct cttcctgcgc gatgcgcatg gtggggagca t
//
LOCUS       DATDOZ010000020         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1585918, whole genome shotgun sequence.
ACCESSION   DATDOZ010000020 DATDOZ010000000
VERSION     DATDOZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585918"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VI541_00360"
     CDS             <1..227
                     /locus_tag="VI541_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HLF69391.1"
                     /translation="AGLHTGECEVRGDDVGGVAIHVAARVLGLAGDGEVLVSQTVKDL
                     TIGSGLDLSDRGSHVLRGVPGEWRLFEVGT"
     gene            273..>2036
                     /locus_tag="VI541_00365"
     CDS             273..>2036
                     /locus_tag="VI541_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF69392.1"
                     /translation="MSQGVVPPVGTVTFLFTDIEGSTRLLRSLGSDYDKLLEDHRRLL
                     FQAAGRQQGYPLGQEGDACFFAFGRATEAVAAALESQLNLTNHPWPPGGEIKVRMGIH
                     AGEVRMTEGRYVGLALHKTARICAAGHGGQILVSAITRDLTDTEGGITFADLGDHVLK
                     DLGRPERILQLVHPELESKFPPLRTIDLLPNNLPSSMTSFVGRHDEIAEVTQLLSSSR
                     LVTLLGPGGAGKTRLALQIAADVLDRYNEGAWMIDLASITTGDAIHQTIAAALRVGEA
                     RADGQGPVQIEEPTGTGAERILSKLRTSQTLLVLDNCEHVITECADFAETLLAQCPNV
                     SILATSRQPLEVPGEARWNVPPLSLPASNMDDLEAVAAHEAVALFLERARLNQSTFAL
                     TTENAGDVVEICRRLDGMPLAIELAAAWVTALSPKQIVERLDDQFKLLATSVRRGPGR
                     QQTLRATIDWSYDMLPQLEQRLLENLTIFTGGFTLEAVEKVCSTEGITPGAVLHLLSQ
                     LINKSLVVRETEGDRYQLLETIRKYGAEKLSEAPQPGVPSASASGQLVFLREGDYWSV
                     GAQEAISRIKHSKGLAYLHVLI"
BASE COUNT          397 a          608 c          636 g          395 t
ORIGIN      
        1 gcgccgggct acacacgggg gaatgcgaag tccgaggcga cgatgtcggc ggtgtcgcta
       61 tccacgtcgc ggcccgcgtg ctgggcctcg caggagacgg cgaagtgctg gtgtcgcaga
      121 cagtgaagga tctcaccatc gggtccggcc tcgacctgtc cgatcgaggt tcgcacgtcc
      181 tgcggggagt accgggggag tggagactgt tcgaggtcgg gacgtgacct aggttgtagc
      241 cttagcacct acgccgggtg gggggtctga ggttgtcgca gggagtagtt ccgccagttg
      301 gaaccgtaac tttcctattc acggacatcg agggctcgac gcgcctgctt cgatcactcg
      361 ggagcgacta cgacaaactt ctcgaggacc accgtcgcct cctcttccag gctgccgggc
      421 gccaacaggg atatccgtta ggtcaagaag gcgatgcgtg tttcttcgct ttcggccgcg
      481 cgaccgaagc ggtcgctgct gcgctcgaat ctcagctgaa ccttactaac catccctggc
      541 cccctggcgg cgagatcaag gtccgcatgg ggatccacgc tggagaggtt cgtatgaccg
      601 aaggtcgata tgtcggcctt gcgcttcaca agacggcaag gatctgcgcg gccggtcatg
      661 gcggacagat cctggtgtcc gccatcaccc gcgacctgac ggacaccgaa ggcgggatca
      721 cgttcgccga ccttggtgat cacgtgctga aggatctcgg ccggcccgaa cggatccttc
      781 agttggtcca tccagaactc gaatcgaagt ttccgccgct ccgaacgatc gaccttctgc
      841 ccaacaacct gccctcttcg atgacctcct tcgtggggag gcatgacgag atcgccgagg
      901 tcactcaact gttgtcatcg tctcgtcttg taacactgct cggccccggc ggagctggca
      961 agacgcgcct tgcactgcag atcgcggcgg acgttttgga tcggtacaac gagggggcat
     1021 ggatgatcga cctggcctcc atcacgaccg gtgatgccat ccatcaaacg atcgcggctg
     1081 cccttcgggt cggtgaagcg cgagccgatg gccaagggcc tgttcagata gaagagccaa
     1141 cagggaccgg agccgagagg atcctcagca aactcaggac gagccaaact ctattggtcc
     1201 tggataactg cgagcacgtc atcacagagt gcgcagactt tgccgagact ttgcttgccc
     1261 agtgccccaa cgtgtcgatc cttgccacga gtcgccagcc gctggaggtg ccgggtgagg
     1321 cgagatggaa cgtgccccca ctgtcgctgc ctgcatcgaa catggacgac ctggaagcag
     1381 tggcggcaca cgaagcagtg gccctgttcc tcgagcgggc aagactgaac cagtcgactt
     1441 tcgccctaac aactgagaat gctggcgacg ttgtcgagat ctgccgcagg ctcgatggca
     1501 tgccattggc tatcgaactc gctgctgcat gggttacggc gttgtcgccg aagcagatcg
     1561 tcgaaaggct cgatgatcag ttcaagcttc ttgcaacgtc ggtgcgacgg gggccggggc
     1621 gccaacagac cctacgcgcc accatcgatt ggagctatga catgctcccg cagctcgagc
     1681 agcggcttct cgagaacctg accatattca cgggcggatt cactctcgag gccgtggaga
     1741 aggtttgttc gaccgaaggg atcactccgg gcgcggtgct tcatcttctc tcgcaactca
     1801 tcaacaagtc cctcgtggtg agagaaaccg aaggcgaccg atatcagttg ttggagacga
     1861 tccgtaagta tggggcggag aaactatcgg aagcccccca gcccggagtt ccatcagcct
     1921 ccgcgtcggg ccagttggtt ttcttgcgcg agggcgacta ttggtccgtt ggggcccagg
     1981 aggcgatatc gcggatcaag cactcgaagg gcctcgccta ccttcatgtc ctgatc
//
LOCUS       DATDOZ010000021         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1064183, whole genome shotgun sequence.
ACCESSION   DATDOZ010000021 DATDOZ010000000
VERSION     DATDOZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064183"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VI541_00370"
     CDS             complement(<1..1094)
                     /locus_tag="VI541_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLF69393.1"
                     /translation="MEETAEDDAARLSDLIAKDQLPRALEMLDSRRPADAADLLESLD
                     DDLLARLVDEWSLTAAAEVLEELDPERRAALAERIPAHKLADILETMPPDEAADFLGE
                     VDPHRRAGLIGRMERDEASDVAELFDYPEDSAGRRMSQDFVAVGEELSADAVIERLRE
                     VPEDIELIYYVYVLGGSEQLKGVVSLRKLLTAPSITTVGSLMETDVVSIRPNDDVEAV
                     VDLVRRYDLLALPVTDDLGRMLGIITVDDVLEAMEEESEQDVLRFAGSINDQAQAITA
                     WPTFRRRLPWLAISTLIELLIAYLLLRPLPRELMAATVAYIPLLIFLGGNAAIQAAAR
                     VLVRLQGPRTEAWSAWKQARRELEAGALLGV"
     gene            complement(1110..1319)
                     /locus_tag="VI541_00375"
     CDS             complement(1110..1319)
                     /locus_tag="VI541_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69394.1"
                     /translation="MPQGSIKEYNVDTKTGLIVSDDGESEWEIDPIAIDTGMFRFFRT
                     GQRVTFDLIEEDSVLRPRNLRIGIV"
     gene            complement(1328..2221)
                     /gene="era"
                     /locus_tag="VI541_00380"
     CDS             complement(1328..2221)
                     /gene="era"
                     /locus_tag="VI541_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLF69395.1"
                     /translation="MSAQPFRSGFVSIAGRPNVGKSTLMNALVGEKVAIASSRPQTTR
                     NALRGILNRETFQIIFVDTPGLHKAVTPLGRRLNSLVSRTISEVDVGVFVGDASEQIG
                     TGDFYAARQILDAGIPALCVLNKADKVQPPRLLEQIEAARKLGAWVDVLTTSARRKTG
                     LNFLVDAIVKLLPPGPALYPQDMVSDQPEELAIAEIIREKVLELTREEIPHSVAVQIE
                     EMEQRENSDIFDVFASIYVERDSQKGILIGRGGKMLKEVGSRARVDLEARVGGRVFLD
                     LRVKVADNWQRDDRALDDFGY"
     gene            complement(2218..>2965)
                     /locus_tag="VI541_00385"
     CDS             complement(2218..>2965)
                     /locus_tag="VI541_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLF69396.1"
                     /translation="VAEKEEVIEAGEREMIHSIFEFTDTVVREVMVPRPDMICVESSA
                     SLEEVLEIAIRKGFSRIPVISDNIDDIVGIVYEKDVIRRLHNGGGRAKVRKAGDIARD
                     ATFVPETKKVAELLREMQRTKTHMVIVVDEYGGTAGLATLEDILEEIVGEISDEYDRD
                     EPAAVRVDPTTVRVSARFSITELNELLDAELPHEEWDSVGGLVAGLLGRVPSAGDGVV
                     LHGIRFEVERVRGRRIEKLLVHHGSDAKDR"
BASE COUNT          559 a          952 c          860 g          594 t
ORIGIN      
        1 actccgagca aggcccccgc ctccaactcc cgacgcgcct gtttccacgc gctccaagcc
       61 tcggtccggg ggccttggag acggaccagc acacgagccg ccgcctggat cgcagcgttt
      121 ccgcccagga agatcaggag aggtatgtag gccaccgtcg ccgccatcag ttcacgcggc
      181 aacgggcgca ggagcagata ggcgataaga agttcgatga gggtacttat cgccagccat
      241 ggaagacggc ggcggaacgt gggccatgcg gtgatggcct gagcctggtc gttgatcgat
      301 ccagcgaagc gaaggacatc ctgttcggac tcctcctcca tcgcttcgag gacatcatcg
      361 acggtgatga tcccgagcat gcggcccagg tcatccgtca ccggcagggc cagcaggtca
      421 tagcgacgga ccagatcgac aaccgcctcg acatcgtcgt tgggtctgat cgaaaccaca
      481 tcggtctcca tcagcgatcc cacggttgtg attgaggggg cggtcaggag cttgcgcagc
      541 gataccacgc ctttgagctg ttcactaccg ccaagcacat agacgtagta gatgagttcg
      601 atatcctcgg ggacctcgcg caaccgctcg atcacagcgt cggcactcaa ctcttcgccc
      661 actgcgacga agtcctgcga catccgtcgg ccagccgaat cctcggggta gtcgaagagt
      721 tccgcaacgt cggaggcctc atcgcgctcc atgcggccga tcaatccagc ccgcctgtgg
      781 gggtcgacct cacctaggaa gtccgccgct tcgtccggcg gcatggtctc gaggatgtct
      841 gcgagcttgt gggccgggat cctctcggcg agagcggctc gtcgttctgg atccagttct
      901 tcgagaacct cggcggcggc ggtgagggac cactcatcga cgagccgggc caaaaggtcg
      961 tcgtccagag attcgagaag gtccgcggca tcagcgggcc ggcgagagtc gagcatctcg
     1021 agagcccggg gcagctggtc tttcgcgatc aggtcgctca gccgagcggc gtcatcctcg
     1081 gcggtctcct ccatggcgag ggcccagctt cagacgatcc cgatgcgcag gttacgggga
     1141 cgcaacacgg aatcttcttc gatcagatcg aatgtgaccc gctgcccggt cctgaagaat
     1201 cggaacatcc ctgtgtctat cgctatcggg tcgatctccc actcgctctc accatcatca
     1261 ctgacgatca gtccagtctt cgtatcgacg ttgtactcct tgatcgaacc ctgaggcatc
     1321 gcccctccta gtacccgaag tcgtccagtg cacggtcatc gcgctgccag ttgtctgcta
     1381 ccttgacgcg aagatcgagg aatacccggc ccccgactcg cgcctcgaga tcaacacgtg
     1441 cgcggctgcc gacctccttg agcatcttac ccccgcgccc gatgaggatg cccttctggg
     1501 agtctctctc cacatagatc gaggcgaaga catcgaagat gtcgctgttc tcacgctgct
     1561 ccatctcctc gatctgcaca gccaccgagt gagggatctc ctcgcgggtc agctctagga
     1621 ccttctctcg gatgatctct gcgatcgcga gttcctcggg ctgatccgaa accatgtcct
     1681 gcggatagag cgcggggcca ggagggagca gcttgacgat ggcatcgacg aggaagttca
     1741 aaccggtctt tcgccgagcc gacgtcgtga ggacgtcgac ccaggccccc agtttgcgag
     1801 cggcttcgat ctgttcgaga agccgtgggg gctgcacctt gtcggccttg ttcagaacac
     1861 agagcgcggg gatcccggca tctaggatct gcctcgccgc atagaagtcg ccagtgccga
     1921 tctgctctga ggcatcgccc acgaacacgc cgacatcgac ctctgagatc gtgcgcgata
     1981 ccagcgagtt gagccttcgc ccaaggggtg tcacggcttt atgaagcccc ggtgtatcga
     2041 cgaagatgat ctggaaagtc tcgcgattca atatccctcg caaggcgttg cgtgtggtct
     2101 gcggtctgct cgatgcgatc gcgaccttct cgccgaccag ggcgttcatc agggtgctct
     2161 ttcccacgtt tgggcgcccg gcgatcgaaa cgaaaccgct gcggaatggt tgggcgctca
     2221 acgatccttc gcatccgagc cgtggtggac gagaagtttc tcgatccgcc tccctcgcac
     2281 cctctcgact tcgaaccgga tgccgtggag tactacaccg tcgcccgccg aaggaacacg
     2341 tccgagcagt ccggcaacga ggcccccgac cgagtcccat tcttcgtgcg ggagctccgc
     2401 gtcgagtaac tcgtttagtt cagtgatact gaacctcgcg ctgaccctga ccgtagttgg
     2461 atcgacgcgc acggccgctg gttcgtcgcg gtcgtactca tcggatatct cgccgacgat
     2521 ctcttcgagg atgtcctcca aggtcgcgag cccggccgtt cctccatact catcgacgac
     2581 gatgaccatg tgcgttttgg tcctctgcat ctcacgaaga agttcagcta ctttcttggt
     2641 ctcgggtacg aaagtggcat ctctagcgat gtctccggct ttgcgtacct tggctcggcc
     2701 cccaccgttg tgaagtcttc ggatcacatc cttctcgtag acgatgccaa cgatgtcgtc
     2761 gatgttgtcc gagatcaccg ggatacgcga aaaccccttg cggatcgcga tctcgagaac
     2821 ctcctcgaga gaagcggacg actcgacaca gatcatgtcc ggccgaggaa ccatgacctc
     2881 ccttacgacg gtgtcggtga actcgaagat cgagtggatc atctcgcgct cgccagcttc
     2941 gatcacctcc tccttctccg cgaca
//
LOCUS       DATDOZ010000022         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_539622, whole genome shotgun sequence.
ACCESSION   DATDOZ010000022 DATDOZ010000000
VERSION     DATDOZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539622"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /gene="rpoN"
                     /locus_tag="VI541_00390"
     CDS             <1..1584
                     /gene="rpoN"
                     /locus_tag="VI541_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLF69397.1"
                     /translation="LRINPRLYQAMDMLYMPMMDLQQHLKTELLTNPFLELVEPEEEE
                     QANDNATADQEKEKEKAEKNDEIDWEEILLNGFDVGGTKQQFEVQEYVEPVTVEVLDL
                     GDHLREQLRFLQLTPRELLLGEELIGHINDEGYLATPLDDVLVSVNQFLASNIPEADD
                     DQEDEETDMVTEVARDTMEVVFSHPSTSTPLPNPGALPQPATRAAPAEPVFEGEVVPG
                     VSYFSAKEVEDSLRIIQRLDPPGIGARDIRECILLQMAEDKAGDPLARQLVEEAFEDL
                     KAHRWNDLAKRYGVEPAQVQGAADTLSRYDPKPGLKYANRSDGYIIPDLVVEKVDEHY
                     HVFLNDTGVPRLRVSRSYQELARDRSKMTAEHREFITTKMNSANWMIQAIEQRRQTML
                     KVMNFIVDRQRAFFEKGVEYLKPLTLREVADVISMHESTVSRVTNEKYVQTPRGVLPL
                     KFFFSSALATASGEEASARSIRAKLEKMVADENTGKPLTDQQIVHLFQEQGIQIARRT
                     VAKYRDQLGILPARMRKRV"
     gene            1584..>2052
                     /locus_tag="VI541_00395"
     CDS             1584..>2052
                     /locus_tag="VI541_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF69398.1"
                     /translation="MSSPRHVDVSLLVPAKDEADNLPEFVRRCSEGLTAGDFSFEVIV
                     VDDGSRDASATILTQLATKHPFLRVVTHRRQLGIADALRSGSEVAAGDIFVFYPADLQ
                     YAPEDIPTLVTPILEGRADIVTGTKQGKYEKAFVSRVYNVLCRWLFGVRVEDLN"
BASE COUNT          470 a          618 c          603 g          361 t
ORIGIN      
        1 ctgcggatca atccgcggct gtaccaggcc atggacatgc tctacatgcc catgatggac
       61 ctccagcagc atctcaagac cgagctgctt accaacccgt tcctcgaatt ggtggaaccc
      121 gaagaggaag aacaagccaa cgacaacgcc acggcggacc aagaaaagga gaaagaaaag
      181 gctgaaaaga acgacgagat cgactgggag gagatcctgc ttaacgggtt cgatgtcggg
      241 ggaacgaagc agcagttcga agtccaggaa tacgtcgaac cggtgaccgt cgaggtcctc
      301 gacctgggcg accatcttcg ggaacagctc cgcttcctgc agctcacccc ccgcgaactg
      361 ttgctgggtg aagaactgat cggccacatc aacgacgagg ggtaccttgc cacgccactc
      421 gatgacgtgc tcgtctcggt caaccagttc cttgcctcaa atatccccga agcggacgac
      481 gatcaagaag acgaagagac cgatatggtg acggaagtcg cgcgcgatac catggaagtc
      541 gtcttctcac atccatcgac ctccacacca ttgcccaacc ctggagccct accgcagccg
      601 gcaacacggg cggcaccggc agagcccgtg ttcgaggggg aagtcgtgcc gggggtgagc
      661 tatttcagcg ccaaggaggt ggaggactct ctgcggatca tccagcggct cgatcctccg
      721 ggcatcgggg cgagagacat ccgcgagtgt atcctcctgc aaatggccga ggacaaggct
      781 ggagacccgt tggcgcggca actggtggaa gaagccttcg aagacctcaa ggcccatcgc
      841 tggaatgacc ttgccaagcg gtacggtgtg gagcctgcac aggttcaggg tgccgctgac
      901 accctctccc gctacgaccc caagccaggc ctcaagtacg ccaatcgctc cgatggctac
      961 atcatccccg atctcgtggt cgagaaggtc gacgagcatt accatgtctt cttgaacgac
     1021 accggcgtgc cgcggctccg cgtgagtcgt tcgtatcagg aactggcccg tgaccgttcg
     1081 aaaatgacgg cagagcaccg cgagtttatc accaccaaaa tgaacagcgc caactggatg
     1141 attcaggcga tcgagcaacg gcgacagacc atgttgaagg tcatgaactt catcgtggat
     1201 cgtcagcgcg cgttcttcga aaagggggtc gaatacctga agcccctcac gttgcgcgag
     1261 gtcgcggacg tgatcagcat gcacgagtcc acggtcagtc gcgtgaccaa cgagaaatac
     1321 gtccaaacgc ctcggggcgt actccccctc aagttcttct tctcgagtgc tctggccacg
     1381 gcctccggcg aggaggcgag tgcccgatcc atccgcgcga aactcgagaa gatggtggcg
     1441 gatgagaaca ccggtaaacc gctcaccgat cagcagattg tccacctctt ccaggagcag
     1501 ggaatccaga ttgcccggcg gaccgtagcg aagtacagag atcaactggg cattctgccg
     1561 gcccgaatgc gaaagcgcgt gtgatgtcgt cccctcgaca tgtggatgtg agtctgctgg
     1621 ttccggcgaa ggatgaggcc gacaacctcc ccgagttcgt ccgacgatgc agtgaaggac
     1681 tcaccgcagg ggacttcagc ttcgaggtca tcgtggtgga cgacggctcc cgcgatgcga
     1741 gcgcgacgat cctaacccag ctcgccacga aacatccgtt ccttcgtgtg gtgacgcatc
     1801 gacgtcagct aggaatcgcg gacgccctca gaagcgggag cgaggtcgcg gccggtgaca
     1861 tctttgtgtt ctatccagcc gacctgcagt atgcaccgga ggacattcca accctggtca
     1921 cccccattct cgagggcaga gcggacatcg tcaccggcac caaacaaggt aagtacgaga
     1981 aggccttcgt ctcacgcgtc tacaatgtgc tctgtcgctg gctctttgga gtgcgcgtcg
     2041 aggatctcaa tt
//
LOCUS       DATDOZ010000023         3764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3157817, whole genome shotgun sequence.
ACCESSION   DATDOZ010000023 DATDOZ010000000
VERSION     DATDOZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3157817"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VI541_00400"
     CDS             complement(<1..720)
                     /locus_tag="VI541_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69399.1"
                     /translation="MRRIAMLMALALGVVLALPALASYHDEPAVGFRTESCALGSNAL
                     PGSGNLLVVDPVLGNGDYTTIVAAVAAASANDYILVRPGTYNEAVVIPATKPGLRIRG
                     TNRNTVILDGGADATHAGALYGFDVRADRVVIENMTAHNYRGTAFYWHGVTGYWGSHL
                     TGYNTGGYGLYAYNARCGQFDHSFGSGNADSAFYIGECFPCEAVIHDVEATGNGLGYS
                     GTNAGGELVIRDSYWHDNGLGI"
     gene            complement(746..1171)
                     /locus_tag="VI541_00405"
     CDS             complement(746..1171)
                     /locus_tag="VI541_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69400.1"
                     /translation="MITSNLSWMTTTRLAMAALLSATLLTIPISAGPALGAGTTHEVR
                     SLANTYIPGDHDISLELRIRQGDSIRYQNFDFAPHNIVSVAQTTTIPPEPLFWSETSD
                     LSHRNVETVNGVPSLQTGSFEFFCTIHESMRGTLHVDPA"
     gene            complement(1183..1533)
                     /locus_tag="VI541_00410"
     CDS             complement(1183..1533)
                     /locus_tag="VI541_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLF69401.1"
                     /translation="MEETRIGSASELVGGKLIGVGGYVVGKSQGAYFAVSRRCRHLGA
                     DLARGSVDREGCLVCPLHASRYNVENGRMVRGPQGIYAKIPGLGAAYKLLTMVIPLRR
                     AQVTEREGILYLES"
     gene            1613..3163
                     /locus_tag="VI541_00415"
     CDS             1613..3163
                     /locus_tag="VI541_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLF69402.1"
                     /translation="MDEVTPVALFVFDRHVDLPRTIECLRADHVKRLLVFADGPRGEA
                     DVEGVERVRALIRSIDWADVTLDAADHNRGLSASIEHGLGTIFDSHERAIVVEDDVCV
                     APGFYAYMNACLDRYADEPRVAGVTGLRYPFDPKGFDNYPYDVFFAPRFSSWGWGTWK
                     RFWMSRETDRNKLSEMALRRGTIYAKGGADLPETIGGYLDGTLEGAWDAGCAANILIN
                     DQLFVWPVRNMIENTGLINGTHSSARPPKWRLEFEGSSTNLRMPDEVKVDPRIMKEFL
                     TFFEPDLSLRQTARRVAGRSKRAVLGLLKGTPRGRPDPKDYTTTDGPMEVPCQREAYL
                     HALNQHVADGDRVLDVGHGLGYGMKLLSTKAGEVYGVDVDLKAVEYARAHVLGSDPKL
                     KEVEHYDGYGLPYGDKTFDLVTCVDVIEHVEDYHRFIDELLRVAKRTVIFSTPNRRPE
                     YTNRDGTPRNHWHLREWSFEELDEILHQHPVVLEWRFIDGPWEGPFTLSERVGPRTLV
                     LLPALHPN"
     gene            3235..3573
                     /gene="trxA"
                     /locus_tag="VI541_00420"
     CDS             3235..3573
                     /gene="trxA"
                     /locus_tag="VI541_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLF69403.1"
                     /translation="MSNLPNVTDQDFTAEVIESGTPVLVDFWAEWCRPCHMVAPEVEA
                     VSEKLAGRLKVVKLDIDENQITAAKYGVVSIPTLILFSAGQEALRLVGARPRTAILAE
                     LETVLGAQAS"
     gene            complement(3570..>3764)
                     /locus_tag="VI541_00425"
     CDS             complement(3570..>3764)
                     /locus_tag="VI541_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69404.1"
                     /translation="RYGIDRWHLARDFGPGDSALHDALGGRGMPITVFYDRSGRKIDF
                     ANGALSEPALRQRLADLYGV"
BASE COUNT          766 a         1074 c         1167 g          757 t
ORIGIN      
        1 tatcccaaga ccgttgtcat gccagtacga gtcgcggatc acgagttccc cgcccgcatt
       61 ggtcccggag tagccgaggc cgttgcccgt cgcctcgaca tcgtggatga ccgcttcaca
      121 tgggaagcac tcgccgatgt aaaaagctga gtcggcgtta ccactgccga atgagtggtc
      181 gaactgcccg cacctcgcgt tgtatgcata caggccatag ccgcctgtgt tgtatcccgt
      241 cagatggctg ccccagtagc cggtaacccc gtgccaatag aaggccgtgc cacgatagtt
      301 gtgagccgtc atgttctcga tcacaacgcg atccgctctg acatcaaaac cgtatagggc
      361 gccggcatgg gttgcatctg ctccgccatc gaggatcacg gtgttgcggt tggttcctct
      421 gatacgaagt cccggcttcg tagcaggtat gacgactgcc tcgttgtagg ttccggggcg
      481 gacgaggatg tagtcgtttg cgctggccgc cgccacagca gcgacgatcg ttgtgtagtc
      541 cccattgccc aggacgggat cgacgacaag tagatttcca ctgcccggta atgcgttcga
      601 gccgagagcg catgactcgg tacggaaccc gacggctggc tcatcgtgat aactggccag
      661 ggcaggaaga gctagtacga cccccaaagc cagggccatc aacatggcga ttcttctcat
      721 ctcactttcc tccgttctgt tctggttagg ccggatctac atgaagagtg ccgcgcatgc
      781 tctcgtggat cgtgcagaag aactcaaagc tccctgtttg gagggaggga acaccgttga
      841 ccgtctcgac atttcggtgt gacaggtccg aagtctcgct ccaaaagagt ggctcaggcg
      901 ggatggtcgt cgtctgagcg accgaaacga tgttgtgcgg ggcgaagtcg aaattctgat
      961 atctgatcga gtcgccctgc ctgatcctta gctccaggga gatgtcgtgg tcacccggga
     1021 tgtaggtgtt cgcaaggctc cggacctcgt gagtcgtgcc cgctcccagg gcgggccccg
     1081 ctgagatcgg aatcgtcagg agggtggccg agaggagcgc agccatcgct aggcgggttg
     1141 tggtcatcca tgacagattc gacgtgatca tcaagattcc tcctagctct ccaggtaaag
     1201 gatgccttca cgctcggtga cctgggcccg cctcagcggg atcaccatcg tcagcagttt
     1261 gtaggcggcc cccaggccgg ggatcttggc gtagatgccc tggggccccc ggaccatccg
     1321 cccgttttcg acgttgtatc gcgaggcatg gaggggacag accagacacc cctcccggtc
     1381 gacgctgccg cgagcgaggt ctgcgccgag gtggcgacag cgtctgctca ctgcgaagta
     1441 ggcaccctgt gacttgccga cgacgtaacc accgacgccg atgagtttgc caccgaccag
     1501 ctcagaagcc gatccgatgc gagtctcttc catgaggctg atgctatttc atcgggggat
     1561 gccgaagcgg cccgcggtca acggctcgag cctgcaacta ggattgtcgg atatggatga
     1621 agtaactccc gtggccctgt tcgttttcga tcgtcatgtg gatctgccca ggaccattga
     1681 gtgccttagg gcggatcacg ttaagaggct gctcgtattc gctgacggac cacgtggtga
     1741 ggccgacgtt gagggcgtag agagagtcag ggccctcata cgctctatcg attgggctga
     1801 cgtaacattg gatgcagccg accacaaccg gggcctgagt gcatcgatcg agcacgggct
     1861 ggggactatc ttcgatagtc atgagagagc catcgtcgtg gaagatgacg tctgcgtcgc
     1921 tcctggcttc tacgcgtata tgaacgcctg cttagatcga tacgcggatg agccacgagt
     1981 ggcaggggta acgggtctgc gttatccctt cgatcccaag ggattcgaca actatccata
     2041 cgatgtcttc ttcgcccccc gcttctcatc gtggggttgg gggacctgga agcggttctg
     2101 gatgtcgcgc gaaaccgatc gcaacaagct gagtgagatg gcgctacgac gaggcaccat
     2161 ctacgcgaag ggcggagccg acctacccga aaccatcgga ggctacttgg atggaacgct
     2221 cgagggtgca tgggatgctg gctgcgcggc aaacatcctg atcaacgatc agctattcgt
     2281 ctggccagtg cgcaacatga tcgagaacac cggcttgatc aacggtacac attcgagcgc
     2341 gaggcccccg aagtggcgct tggagttcga ggggtcttcc acaaaccttc gcatgccaga
     2401 tgaggtgaag gtcgatccac ggatcatgaa ggagttcttg accttcttcg agccggacct
     2461 cagcctgagg cagacggccc gcagggtcgc tggacggtcc aagcgggctg tgctggggct
     2521 tctcaaaggg accccccgag gaaggccgga cccgaaggac tacacaacca ccgatggtcc
     2581 gatggaggtt ccctgtcagc gagaagccta cctgcacgcg ctcaaccagc atgtcgccga
     2641 tggtgaccga gtgttggatg tcgggcatgg tctcggctac gggatgaaac tgctatcaac
     2701 aaaggccggt gaggtctacg gtgtcgatgt cgacctcaag gccgtcgagt atgcgagggc
     2761 ccatgtgctg ggcagcgatc ccaaactgaa agaggtggag cactacgacg gttacgggtt
     2821 gccatacggt gacaagacgt tcgaccttgt cacctgtgtg gacgtgatcg agcatgtcga
     2881 ggactatcac cgcttcatcg acgagttgct gcgagtagcg aagcgcaccg tgatcttcag
     2941 tactccgaac cgacggcccg agtatacgaa cagagacggg acgcccagga accactggca
     3001 tctgcgcgag tggagcttcg aagaactcga cgagatcctt catcaacatc cggtggtcct
     3061 ggagtggcgg ttcatcgatg gcccttggga gggacccttc actctcagcg aacgagttgg
     3121 cccccgcact ttggtgctat tacccgccct tcatccaaac tgaccggatc cagggaataa
     3181 tggaggaacg cggcggcgtg agtaacttga gatcgaacga tcgggaggtt cttgatgtct
     3241 aacttgccaa atgtcacaga ccaggatttc acggccgagg tgatcgaatc agggacacct
     3301 gtactagtcg atttttgggc cgagtggtgt cgcccctgcc acatggttgc acccgaggtc
     3361 gaggctgtga gcgagaagct cgccggtcgc ctgaaggtgg tcaaactcga tatcgacgag
     3421 aaccagatca ccgctgccaa gtatggggtg gtctctatcc caactttgat cctcttctcg
     3481 gcagggcaag aagccttgcg cctcgtgggg gcccggccgc ggaccgcgat ccttgcggag
     3541 ctcgagacgg tgctgggcgc gcaggcgagc tagaccccgt agaggtcggc gagtctctgt
     3601 ctcaacgcag gttcggacaa cgcgccgtta gcgaagtcga tctttctacc agagcgatcg
     3661 tagaacaccg tgatgggcat gccgcgacct ccgagagcgt catgcagcgc actgtcgccg
     3721 ggtccgaagt ccctcgcaag atgccagcgg tcgatcccat acct
//
LOCUS       DATDOZ010000024         4084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1588279, whole genome shotgun sequence.
ACCESSION   DATDOZ010000024 DATDOZ010000000
VERSION     DATDOZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588279"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VI541_00430"
     CDS             complement(<1..260)
                     /locus_tag="VI541_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF69405.1"
                     /translation="MGWQGRRVLVTGGEGFIGSHLVERLVREGAEVRVFAYYNSFGKW
                     GWLDEVEGAFEVMPGDVRDSERVFTAVDGMDTVFHLAALIGIP"
     gene            complement(260..1252)
                     /locus_tag="VI541_00435"
     CDS             complement(260..1252)
                     /locus_tag="VI541_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLF69406.1"
                     /translation="MKRFIVKPSARIDAVFERAASLGVLFIVDDGVMVGWLTDAAIRR
                     HILDGGSLDGVVADLATAVGKSKAQVTLEPESAPVSAVIEGGRLVSIAVRPPIPPPIA
                     VVMAGGRGQRLRPITDKVPKPLLKVGNSSIVERILRGLGAAGITDCYLSVNYKAREFE
                     SRLRDGKQLGIELRYLKEESKLGTAGALSLLPKKPKVPVLVTNADILTRLDYARIVEF
                     HRTEGGAATMAAIPYVTQVPYGVARNQGARLVGIEEKPDISVLCNAGIYVLEPEALSV
                     VRKGKPLDMPDLLSRLVEKGKQVNVFPVIERWFDIGSPEDFKQVLMEFATYEEE"
     gene            complement(1254..2378)
                     /gene="murG"
                     /locus_tag="VI541_00440"
     CDS             complement(1254..2378)
                     /gene="murG"
                     /locus_tag="VI541_00440"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HLF69407.1"
                     /translation="MSAVKARSVVIAAGGTGGHIFPGLALADAIKRAAPGTRIAFVGT
                     KRGLESRLVVDAGYPLDLIDMVPMVRDQLVRFPFALVRSVVQARRILRERGADVAVGM
                     GGYSSAPLMIAARTARIPSLIHESGAIPGRANLFAARFTGNIALSFATAAKSFKRARV
                     VGMPISDDLSRTPDPAQRAVHRATFGVPHGARVVLVIGGSQGATTLNRAAIGLAKRWL
                     TREDLFIILKTGKAHLADIEREIDALGAGERIRCLSFIEKMSEAYGVADVAVCRAGAG
                     TIAELAAVGLPAVLIPYPHAPYDHQAVNASVLVETGGAEMVRDHEATPDRLGGIIEKM
                     LDDPEALMARRESLRSVAHPQAADELARWVLELGGLVASG"
     gene            complement(2383..2757)
                     /locus_tag="VI541_00445"
     CDS             complement(2383..2757)
                     /locus_tag="VI541_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HLF69408.1"
                     /translation="MSPTNGDKVKEAKIRRIQQDQRHSIQMMADRILVASPKELGERR
                     SRGGILIPATVASETRRAIWGEVIDVGPMVRNVLAGDLVLFVPETAAEVEIREEEYIV
                     LRERDIHAVASERSESRTGLYL"
     gene            complement(2754..3149)
                     /locus_tag="VI541_00450"
     CDS             complement(2754..3149)
                     /locus_tag="VI541_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006547640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLF69409.1"
                     /translation="MSERARDRDPRRAVYIISVVADLAGVHPQTLRMYERRGLLEPKR
                     TAGNSRRYSEQDVDQLRRIQELTQDEGVNLAGVRRILELERELARAREHVHRLQRRVA
                     HAERNAARAKRGVMVRLVDVRSIFDRPNA"
     gene            complement(3146..4048)
                     /locus_tag="VI541_00455"
     CDS             complement(3146..4048)
                     /locus_tag="VI541_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HLF69410.1"
                     /translation="MNQREWVEKDYYAILGVDKSASEAQIRKAYRKLAQKHHPDANQG
                     DPKAEERFKEISQANDVIGDPVKRREYDQVRAAFGSGTRFQGFGGGQRVNVEDVFGSG
                     AGGFEDLFGSLFGGSRGPQKGPDLETQATLGFEEALDGTTVTLNLSDGAESRTIKARI
                     PAGVNDGARIRLPGKGGASPYGGPRGDLFIRVAVTPHRFFGRKGKDLTLQLPVTFVEA
                     TLGAEVEVPTLNGHAVLLKVPVGTSSGKTFRVRGKGPAGAPDILVTLQVAVPKRLSKD
                     QKELLRRFAELEGESPRESFEEPR"
BASE COUNT          767 a         1362 c         1182 g          773 t
ORIGIN      
        1 gggatcccga tgagggcagc aagatggaag accgtatcca tgccatcgac cgcggtgaat
       61 actcgctcgg aatcgcgaac atctccaggc atcacctcga aggcaccctc tacttcatcg
      121 agccagcccc acttcccgaa ggagttgtag taggcgaaga cgcgaacctc ggccccctct
      181 cgcacgagac gctcaacgag gtgactgccg atgaacccct cgccacccgt gacgagcacc
      241 ctgcgaccct gccagcccat cactcctcct cgtatgtggc gaactccatc aagacctgct
      301 tgaaatcctc ggggctcccg atatcgaacc agcgctcgat gacggggaag acattaacct
      361 gcttaccctt ctcgaccaga cgcgagagca agtccggcat gtcgaggggt ttgccctttc
      421 gcacaaccga gagagcctca ggttcgagga cgtagatacc tgcattgcac agaacactga
      481 tgtccggctt ctcctcgata cccacgaggc gggccccctg gttacgggcg acaccgtacg
      541 ggacctgcgt gacgtatggg atcgcagcca tagtagcggc gccgccttcg gtgcgatgga
      601 actccacgat ccgcgcatag tcgagtcgag tgagtatgtc tgcgttggtg acgagtacgg
      661 gtaccttggg tttcttcggc aagagcgaga gcgctcccgc ggtgccgagc ttgctctctt
      721 ccttcagata acgcagttcg ataccgagct gcttgccatc acgaaggcgc gactcgaact
      781 ctcgcgcttt gtagttcacc gagagataac agtcggtgat gcctgctgcg ccgagaccgc
      841 gcaggatccg ttcgacgatc gaagagttgc cgactttgag cagaggcttg ggcaccttgt
      901 cagtgatggg ccgcaaccgc tggccacggc ccccagccat cacgactgct atgggtggcg
      961 gtatcggggg ccgcaccgcg atcgatacga gacgcccacc ctcgatcacc gccgatacgg
     1021 gggccgactc aggctcaagc gtgacctgag ccttgctttt gccgaccgcg gtagcgagat
     1081 cggcgaccac accgtcgaga gatcctccat caaggatgtg acgacggatc gcggcatctg
     1141 ttagccaccc gaccattact ccgtcgtcga cgatgaagag aacacccagc gaggccgcgc
     1201 gctcgaacac tgcgtcgatg cgcgcgctgg gcttcactat gaagcgcttc aactcagccg
     1261 ctcgcaacca gccccccaag ctccaagacc cagcgcgcaa gttcgtcggc cgcctgcgga
     1321 tgtgcgacag atcgcagact ctcgcgacga gccatcagag cctccggatc gtcaagcatc
     1381 ttctcgatga tgcccccgag cctatccggc gtagcctcgt ggtcacggac catctcggcc
     1441 cccccggtct cgacgagcac cgaagcattc acagcctggt ggtcgtaggg ggcatgcgga
     1501 tacgggatca gcaccgcggg aagtccgaca gccgccaact cggcgatggt gcctgccccg
     1561 gcacggcaga ccgcaacatc ggcaacgccg taggcctcgc tcatcttctc gatgaagctc
     1621 aaacaacgga tgcgctctcc cgcgccgaga gcatcgatct cgcgctcgat gtctgcgaga
     1681 tgcgccttcc ctgtcttgag gatgatgaag aggtcctcac gggtgagcca ccgcttcgcc
     1741 agccctatcg cggcgcggtt caatgttgtg gcaccctggc tgccccctat caccaggaca
     1801 accctcgcgc cgtgggggac cccaaaggtc gctcggtgca cggccctctg agcggggtca
     1861 ggagtacgcg agaggtcatc cgatatcggc atcccgacga cacgcgcccg cttgaagctc
     1921 ttggctgctg tcgcgaacga gagtgcgatg ttgccagtga aacgtgccgc gaagagattg
     1981 gctcggcccg ggatcgcgcc cgactcatgg atgagtgatg gtatccgggc ggtgcgcgct
     2041 gcgatcatca gcggagccga cgaatacccc cccatcccca ctgcaacatc ggctcctctc
     2101 tcgcgaagga tcctccgcgc ttggaccacc gaccgcacga gggcgaatgg gaagcgcacg
     2161 agttggtcgc gaaccatggg gaccatgtcg atgaggtcga ggggataacc cgcgtcgacc
     2221 accagtctgc tctcgagccc gcgtttcgta ccgacgaaag cgatccgtgt gccgggggcc
     2281 gctcttttga tcgcatctgc cagagccagc ccagggaaga tatgtccgcc agtgccaccg
     2341 gccgcgataa cgacggatcg cgccttgacg gcactcacaa cttcagaggt agagaccggt
     2401 gcggctctcg gaacgctccg atgccacggc atggatgtcg cgttcacgaa ggacgatgta
     2461 ctcctcttcg cggatctcga cctcggctgc tgtctccggc acgaagagca cgaggtcacc
     2521 ggcgagcacg tttcggacca tgggcccaac atcgatgacc tcgccccaga tcgctcgcct
     2581 cgtctcggac gcgacggtgg cggggatgag tatcccgccg cgactgcgac gttcccccaa
     2641 ctccttaggc gacgcaacga ggatccggtc ggccatcatc tggatcgagt gtctctggtc
     2701 ctgctggatc cttctgatct tcgcctcttt caccttgtct ccattcgttg ggctcatgcg
     2761 ttgggtcgat cgaagatgct gcgcacatcg actagcctaa ccatcacgcc ccgcttcgcg
     2821 cgtgcggcgt ttcgctcggc gtgagcgacg cgccgctgaa gtcgatgaac atgctctcgg
     2881 gccctcgcaa gctcccgctc caactcgagg atccgccgga ctccagcgag attgaccccc
     2941 tcgtcctgag tgagctcctg gatgcggcgc aactggtcga catcctgctc cgagtagcgt
     3001 cgactgttcc ccgcggtcct ctttggttcg agaaggcccc tgcgctcgta catcctcaat
     3061 gtctgcgggt gcacgccggc aagatcggcg acgacgctga tgatgtacac cgctcgacgc
     3121 ggatcgcgat ctcgcgctcg ctcgctcatc tcggctcctc gaagctctct ctcggtgact
     3181 cgccctccaa ctcggcgaac ctgcggagca actccttctg atccttggag agcctcttag
     3241 gaacggccac ctgcagagtg accaggatat ccggggcgcc cgcaggacct ttgccgcgca
     3301 ctcgaaaggt cttgcccgac gaggtaccca ccggaacctt gagcaacacc gcgtgaccat
     3361 tgagcgttgg cacttcgacc tcggccccca aggtggcctc gacgaatgtc actggaagtt
     3421 ggagcgtgag atccttacct ttgcggccga agaatcggtg tggtgttacc gcaaccctga
     3481 tgaataggtc gcctcgcgga ccaccgtacg ggcttgcccc tcccttgccg ggcaaacgga
     3541 tccgggctcc gtcgttgact ccggccggga tgcgggcctt gatggtccgt gactccgctc
     3601 catcagagag attcagcgtg acggtggttc catcgagagc ctcttcgaaa cctagagtcg
     3661 cctgcgtctc gagatcgggg cccttctggg gcccacgaga accgccgaac agagaaccga
     3721 aaagatcctc gaacccaccc gctccggagc cgaaaacatc ctcaacgttg accctctggc
     3781 ccccgccgaa accttggaaa cgtgtaccgc tgccgaaagc ggccctgacc tgatcgtact
     3841 cacggcgctt gaccggatcg ccgatcacat cgtttgcctg actgatctcc ttgaatcgct
     3901 cttcagcctt agggtcacct tggttggcgt ccgggtgatg tttctgagcc agtttcctat
     3961 aggccttgcg gatctgcgct tcagacgcgc tcttatcgac gccgaggatg gcgtagtagt
     4021 ccttctccac ccactcgcgc tggttcactc agatccatcg gccttctggg taactttgac
     4081 catc
//
LOCUS       DATDOZ010000025         2952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3158587, whole genome shotgun sequence.
ACCESSION   DATDOZ010000025 DATDOZ010000000
VERSION     DATDOZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3158587"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VI541_00460"
     CDS             complement(<1..1035)
                     /locus_tag="VI541_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLF69411.1"
                     /translation="MKRFFAGVAAAAVGLGIVYAISTLFPVVPYPPVALAGILRDAAP
                     GGVATALIEGLKHTAKSLLSLLVHIGILASVGGAGLWVSAVRESTRRARRAAAVGAAT
                     ALFQIGLVAISPEHFGVLAVITYLGAAYFVGRVLSGSSLAVGFRRQVRDGETPLDAIQ
                     RSRRRFIVRALSAVVGVIAGGAAMLRVGGRKTPIDLKLAEPAEPVVPPARDPSFPNVP
                     GLSSEITSVDDFYNVDINIIKPAVDHTTWRLKIHGLVESPYSLSYRTMQSSFEVVELA
                     HTLSCISNEVGGDLISTAIWRGVRLRDVLRRAGLRDGVVDVVFRAADGYSDSIPLTKA
                     LEETTLLVFGM"
     gene            complement(1060..2028)
                     /locus_tag="VI541_00465"
     CDS             complement(1060..2028)
                     /locus_tag="VI541_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69412.1"
                     /translation="MIDPLDMPTLFDRIDRGLLRFTGPKAAWFLDQLVTNDIEHLDDE
                     RGAEALLLTPKGKITSAMRVIVNGGSVYADVAADHSKSLTVFLEGRIFATQVEVEDVS
                     DEFHLLSVFGPRSRETVGEVLQRMHMPELEHENCAFGGGLLISVARPVDGVDLWVRAE
                     RLDEVRSSLLKGGISPGSEDLLTDIQIISGFAVMGRDYDETFLTQEAAMEHAVHFHKG
                     CYLGQEAVAMAQRGRVKKRLRHLVFDGRGAVGPVENEGDVVGEVKSFAAVGGQSFAIA
                     SIKTSVELGSRVKVGEAMASVHELPGTAEGERGPDARALREKLTAE"
     gene            complement(2021..2620)
                     /locus_tag="VI541_00470"
     CDS             complement(2021..2620)
                     /locus_tag="VI541_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HLF69413.1"
                     /translation="MFERFTDRARKLIVLAQHEADRSGQRRIGTEHILLALLEEKQSR
                     ASRILIDRGVTRESLVPIISDLPELEPVDEADGMRFTIDAKAVFALGYEESSALGHEF
                     VAPEHLLLGLLADVEGDAAKVMRYTADSVSRIREEVIDAVSGDGSKVCTKCGAAIEEF
                     GKVQPLDLNVEQGEPIKVFAYFCGECRSTYGLVRGGTDD"
     gene            complement(2645..>2952)
                     /locus_tag="VI541_00475"
     CDS             complement(2645..>2952)
                     /locus_tag="VI541_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69414.1"
                     /translation="IADMYLGAEHSRSLLYYAAWAADQATPEADRRGPLNRAAEKAIR
                     RAAIWSSEAALRSTADAIQVHGGIGFTWEHDLHMYFKRARSNHALLPDTASLAIANV"
BASE COUNT          582 a          961 c          880 g          529 t
ORIGIN      
        1 catcccaaag accagcagag ttgtctcttc gagggccttc gtcagtggga tcgaatccga
       61 gtacccgtca gccgcgcgga agacgacatc gacgactcca tcgcgcaggc ccgcgcgtcg
      121 cagcacatcg cgcaggcgaa ctccgcgcca gatcgcggtt gagatgaggt ccccgccgac
      181 ctcgttcgat atacaggaga gcgtgtgtgc cagctccacg acctcgaaag agctctgcat
      241 ggttcggtag ctcagtgagt atggcgactc gactaggccg tggatcttca atctccaagt
      301 cgtgtgatcc acggcaggtt tgatgatgtt gatatcgacg ttgtagaagt cgtcgaccga
      361 ggtgatctcg gaagataagc cgggcacgtt cgggaacgac ggatcgcgtg ctggaggaac
      421 gaccggttca gcaggctcgg cgagtttcag gtcgataggc gtcttgcgcc caccaaccct
      481 cagcatcgcg gcccccccgg cgatcacccc gaccaccgcg gacagcgctc tgactatgaa
      541 ccggcgcctc gatcgctgga tcgcatcgag cggggtttca ccatcgcgga cctggcgacg
      601 aaagcccacg gctagggagg agcccgacag gacgcgaccg acgaagtagg cagctccgag
      661 atacgtgatc accgcaagga ccccgaagtg ttctggcgat atcgcaacga ggccgatctg
      721 gaagagtgcg gttgcggcgc cgacggcggc ggctcttcga gctctccgag tgctctctct
      781 cacagcgctc acccagaggc cagctccacc gacagaggcc agtatcccga tgtggacgag
      841 cagcgagaga aggctcttgg ccgtgtgttt gaggccctcg atcagggccg tggccactcc
      901 gcccggtgct gcatcgcgca ggatcccggc cagagccacg ggtggatagg gcacgaccgg
      961 gaagagggtc gagatcgcat agacgattcc aagacccacg gctgccgctg ccacgcccgc
     1021 aaagaaccgt ttcaagtcag gcgatccagt cgatgcgagt cactcggccg tcagtttctc
     1081 gcgcaaggcc cgcgcatcgg gcccccgctc tccctccgcc gtgcccggca actcatgaac
     1141 cgaggccatg gcttcgccca ccttaaccct tgaccccaac tcgaccgagg tcttgatcga
     1201 agcgatggcg aacgactggc cgcccacagc cgcgaagctc ttgacctcac cgacgacatc
     1261 gccctcgttc tcaaccgggc ccacggcacc acgaccatcg aagaccaggt ggcgcagtcg
     1321 cttcttgacc cgtcctcgct gagccatcgc gacagcctcc tgcccgagat agcacccctt
     1381 gtggaagtgg accgcgtgct ccatcgcggc ttcctgcgtg agaaacgtct catcgtagtc
     1441 acggcccatc acggcgaaac cgctgatgat ctggatatcg gtcaacagat cctctgagcc
     1501 tggcgagatg ccgcccttga gcagagagga cctcacctcg tccagcctct cggccctgac
     1561 ccagaggtcc accccatcga cgggcctcgc gaccgatatc agaagtccgc ccccgaatgc
     1621 gcagttctca tgctcgagct cgggcatgtg catgcgttgc aacacttcgc ccaccgtctc
     1681 gcgcgaacgg gggccgaaca ccgaaagaag atggaactcg tcagacacat cttcgacttc
     1741 gacctgcgtt gcgaatatcc gcccttcgag gaatacggtg agcgacttcg agtgatctgc
     1801 cgcaacatca gcatagacgg agcctccgtt gacgatgacg cgcatcgcag atgtgatctt
     1861 gcccttcggc gtgagaagga gggcttcggc ccccctctcg tcgtcgagat gctcgatgtc
     1921 attcgtcacc aactgatcca ggaaccaggc agccttgggg ccagtgaatc gcagcaaccc
     1981 gcggtcgatg cggtcgaaca acgtcggcat gtcgagagga tcaatcatca gtaccgcccc
     2041 gcaccaaccc ataggtcgat cgacactcgc cgcagaagta ggcgaacacc ttgatcggct
     2101 caccctgttc aacgttcagg tcgagcggct ggaccttgcc gaactcctcg atggcggcgc
     2161 cgcacttcgt gcagaccttc gagccatccc cagagacggc gtcgatcacc tcttcacgga
     2221 tgcggctgac cgagtcggcc gtatatctca tgaccttggc ggcgtctccc tcgacatccg
     2281 caagcagtcc caacagtagg tgttccggag caacgaactc gtgaccgaga gcggaagact
     2341 cttcgtagcc gagcgcgaag acggccttcg catcgatggt gaacctcatc ccatcggcct
     2401 cgtcgacggg ctcgagctca gggaggtcgc tgatgatcgg gaccagactc tctcgggtca
     2461 ctcccctgtc gatgagtatc cgcgaggccc tcgactgttt ctcttcgagc agggctaaca
     2521 ggatgtgctc ggtcccgatc cttcgctgac cgcttcgatc ggcctcgtgt tgagcaagga
     2581 cgatgagctt gcgggcccta tctgtgaacc gctcgaacat acctcaaggc tacagcggga
     2641 atgatcagac gttggctata gccagagaag ccgtgtccgg gagcagggcg tggttggaac
     2701 gagcccgctt gaaatacatg tgcagatcat gttcccaggt gaaaccgatg cctccgtgca
     2761 cctggatggc atcggcggtg ctgcggagcg cagcctcgct cgaccagatg gctgcccggc
     2821 ggatcgcctt ctcggctgcg cggttgagtg gacctcttcg atcggcttct ggggtcgcct
     2881 ggtcggcggc ccatgccgcg tagtagagga gggaccgcga gtgctcggcc cccagataca
     2941 tgtcggcgat ct
//
LOCUS       DATDOZ010000026         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2634843, whole genome shotgun sequence.
ACCESSION   DATDOZ010000026 DATDOZ010000000
VERSION     DATDOZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634843"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2646
                     /locus_tag="VI541_00480"
     CDS             <1..2646
                     /locus_tag="VI541_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLF69415.1"
                     /translation="IHLHHREYNIRVVNNSWGGAGDWDPESSITKAVQKLVNEDRIVV
                     VFAAGNSGGNGSNIQTSVWGNIPEVIQVANYYDRTGWLDATSSRGKKSMERTWPDVAA
                     PGTEIISTAATGKPVTYVGNYQDAVINEISGYGEPDVITAPVPQPIDANLAGKDVIVG
                     NYASFTGTSMASPFISGVVALILQANPALEPEQVKEILRATADMPPGRTYRSDGFAVG
                     TGVVDAAEAVAVALRMKDGADMTGAMRTAYVDSSSAPWHINEQLPRMVEIDSPNPNSD
                     VAGKVHVTGRFLSGGLTDNTIPFMPPPPQILTGDPHIYQGGVIDPFGLFGSYAVIPGE
                     PVGLSVRSITSNDATVKLKQETFRVTYRITRGAEVLFGPADAVVAADGSALRSDLDWD
                     VPADAPPGDYVFESTGHVAGAVYQLENLPFSIGVPVVGSASGTRPGVPIRAGTPFSEN
                     STIFFENDFETNADGWTVEQNGTGPGLLTSWTLIDADTSTLTWNGAHSGSKTFYVGDS
                     LAPDQVNGLYYEDLSNVSLVSPVIDLAKALSAELSYWRSGMSERDFDLLRVLVAPEGS
                     QTWTKVDETSGDFLSGGGAWEPSTIDLSAYAGQAVRLKFEFTSDETSLPPNLVGWYID
                     DVAVSGTSGTGLLIKPSLDLDLDQGIGSLKTSINYSALSSSPIKQYELDFGDGTPLIV
                     TKEPGTAVHTYTGGSWITTLKVVTEGGSASVSQLVDVARLGAIEVRTGSGPWIDAGPA
                     LSADKSFAVDLDLASLSAGGVQLQARHCEGVGACIFDSVPVRLVVVGPVAVPSIKPST
                     KPRRAPVAVPAVQSEMTTEPSASAPASTVEPTPSASPAVDASDDPVVKVQGWSASLRA
                     TSLLLVALVAASLGFVLGRRRRRPS"
BASE COUNT          554 a          855 c          866 g          584 t
ORIGIN      
        1 atccatctgc atcatcgcga atacaacata agagtggtca ataactcctg gggcggggct
       61 ggggattggg atcccgagag ttcgataaca aaggccgttc agaagttggt gaatgaggac
      121 aggatcgttg tggtgttcgc cgctggcaac agtggtggta acggttcgaa catccagacc
      181 tcagtgtggg gcaatatccc agaggtcatt caggtcgcta actactacga ccgcactggc
      241 tggctcgacg cgaccagttc gcgaggtaag aagtcgatgg agaggacctg gcccgacgtc
      301 gcggcccccg gaaccgagat catctcaaca gctgccactg ggaagcctgt gacctacgtc
      361 ggtaactatc aagacgccgt gataaatgag atatccgggt acggcgaacc cgatgtcatc
      421 acggcccccg tgccccagcc catcgacgct aacctcgcag gcaaagatgt gatcgttggc
      481 aactatgcat ccttcaccgg caccagcatg gcttctccat tcatctcggg agtcgttgcc
      541 ctgatcctgc aggcgaatcc cgcgctggag cctgagcagg tgaaggagat cctgcgggct
      601 accgccgaca tgcctcccgg tcgcacctat cgcagcgatg ggttcgcagt tggtaccggg
      661 gtcgtcgatg cggcagaagc ggtcgcagtg gctctgagga tgaaggatgg agcggacatg
      721 actggggcta tgcgcaccgc gtatgtcgat tcatcctcgg cgccatggca catcaacgaa
      781 cagctcccac gcatggtcga gatcgactcg ccgaacccca actcagatgt cgcgggcaag
      841 gtccatgtga caggtcgctt cctgtctggc ggtttgaccg acaacaccat cccgttcatg
      901 cccccgcccc cacagatatt gactggtgat ccccacatct atcaaggggg agtgatcgac
      961 ccgtttggac tatttggaag ctacgcggtg attccaggtg aaccggtggg cctaagtgtc
     1021 cgcagtatca ccagcaacga cgcgaccgtg aagctgaagc aggagacctt cagggttacc
     1081 tatcggatca cccgcggggc ggaggtactc ttcggacctg ccgatgcggt ggttgcagca
     1141 gatggatccg cgctccgcag cgacctagac tgggatgtcc cggcggacgc gccgcccggg
     1201 gactatgtct tcgagtcgac aggtcacgtc gcaggcgccg tgtatcaact cgagaacctt
     1261 cccttctcta tcggtgtccc agtcgtcggt tcggcgtcgg ggactcggcc tggggtcccg
     1321 atcagggctg gcacgccgtt cagcgagaac tcgacgatct tcttcgagaa tgacttcgag
     1381 acgaacgccg acggctggac agtggaacag aacgggacag gcccgggcct gctcacctcc
     1441 tggactctta tcgacgctga cacctcgacc ctcacctgga atggtgccca ctcggggagt
     1501 aagacctttt acgtgggtga ctcccttgca ccggatcaag tgaacggtct gtactacgag
     1561 gatctctcga acgttagcct cgtttcacct gtcatcgact tggcgaaagc attgtccgcg
     1621 gagctcagtt actggcggag cgggatgtcg gaacgtgact tcgacctact gcgcgtactc
     1681 gtcgcccccg aaggatcaca gacctggacc aaggttgatg agacgtcagg cgactttctg
     1741 agcggaggcg gggcatggga gccgtcaacc atcgatctga gcgcatacgc tggacaggct
     1801 gtccggctga agttcgagtt cacgagcgat gagacgagcc tgccaccgaa cctggtgggt
     1861 tggtacatcg atgatgttgc cgtctcgggg acctccggca ctggcctctt gatcaagccg
     1921 tcactggatc ttgatctcga ccagggcatc ggttctctca agaccagcat caattactcg
     1981 gctctgtcta gttcgccgat caagcagtac gaactcgact tcggcgacgg cacgcccttg
     2041 atcgtgacca aggagccagg gacggctgtt cacacctaca ccggcggatc gtggataaca
     2101 acgctgaagg tcgtgaccga gggtgggtct gctagcgtct cgcaactggt cgatgtcgca
     2161 cgtctcggcg cgatcgaggt caggactgga tccgggccct ggatcgatgc gggcccggcg
     2221 ctctctgccg acaagtcttt cgctgtcgac ctggaccttg cgtcactgtc agcggggggt
     2281 gttcagcttc aagctcggca ctgtgaagga gtcggtgcct gcatattcga ctcggtgccc
     2341 gttaggctgg tcgttgttgg tcctgtcgct gtgccttcca tcaagccctc aaccaagcct
     2401 cggcgcgcgc cggtagcagt cccagcggtg cagagcgaga tgaccaccga gccctctgcg
     2461 agtgcccctg cttcgaccgt agaaccgacc ccatctgcca gtccggcagt agatgcctca
     2521 gatgatcccg tcgtgaaggt tcaggggtgg agcgcatcac tgcgagctac ctctctactg
     2581 ctggtggccc tggtagcagc ctcgctcggc ttcgtcctcg gacggagacg tcgccgcccg
     2641 agttagccgc cctttctgtc tcggggggcg aagcaggaac ttgccctgtt agtgtcgaac
     2701 ccgtctaggt gttcgtccac taacttgaga ggtgctgaat gctcgtcacg ccacgcaaac
     2761 ttctgatcat tgcagtagta gtggcttcgg cctccgtact ggttcctgcg ggtgcccata
     2821 cttcaggagc accggtcaga cgtccctgtg acccactcg
//
LOCUS       DATDOZ010000027         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2374103, whole genome shotgun sequence.
ACCESSION   DATDOZ010000027 DATDOZ010000000
VERSION     DATDOZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374103"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VI541_00485"
     CDS             complement(<1..379)
                     /locus_tag="VI541_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF69416.1"
                     /translation="MSVLTIMGLSLRRVTRDRQALFFMILLPVLITLIIGVSIFKSVD
                     TLSVGLVTRDSAGALSKELASQIRSTKGFQVEELDSVEAARKAIRRSNILAAVIIPKD
                     YDAALRRGDRVSVEILVDPVRNAP"
     gene            complement(376..1554)
                     /locus_tag="VI541_00490"
     CDS             complement(376..1554)
                     /locus_tag="VI541_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF69417.1"
                     /translation="MGAIVIAIKDIKLRIRDRSFFIQGFIAPLALATIINFAFAGNGG
                     SFHPNYIVVDLDHGPLAAGFVDQVLKGPALKDFMTLSTKSTEAQARPILDAGAASAAF
                     VIPKGFSQSILTGQVANLKVLRSASSQIGADVAIAISNAFISQINANRLSVATALPDP
                     DNATPEEVGALVPLLQNIRVPIELRQGKVGTREVKPAAYFGPSMAIFFLFFTVQFGAR
                     SLIAEKREGTLARLAASPTSTTSILLGKLLSVFVVGLTSLGALLVAVTFIFGANLGDH
                     LAVAAIAVAIVIAAVGVTAMVISFTKTAEQADAFGAMATMALTLLGGNFIPLNQAPEL
                     FRKLSLLTPNGWALRSFTDLIADGGGVSTILPAVGVILAFGVVGLTVAAFRSRKLVVA
                     "
     gene            complement(1554..>2297)
                     /locus_tag="VI541_00495"
     CDS             complement(1554..>2297)
                     /locus_tag="VI541_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69418.1"
                     /translation="QPMTTRSTETRRSIGYVPQEIALYPDLSGAENLRFWGRLYSLSG
                     KELKARVAHVLEVVGLTDRAGDRVSEYSGGMKRRLNIAAGLLHNPALLVLDEPTVGVD
                     PQSRNAILENVETLNAQGMSVLYTSHYMEEVERLCDRVGIIDEGKLIAEGTRRQLVEQ
                     LGQRDRVEIAATGNIETLARSLGQVEGISEVVAHEHGIRLLAGQGSAALPRILEHAAS
                     AGVTVTGAEVIEPDLEAVFLHLTGKALRD"
BASE COUNT          451 a          679 c          746 g          421 t
ORIGIN      
        1 tcggcgcgtt tcgaaccgga tccaccagga tctcaacgct caccctgtca ccacgccgaa
       61 gggcggcgtc gtaatccttg gggatgatca cagccgcgag gatgttcgag cggcggatgg
      121 ctttgcgcgc agcctcgaca ctgtcgagtt cctcgacctg gaatcccttg gtggagcgga
      181 tctgcgaggc cagttctttg ctgagggcgc cagcgctgtc gcgcgtaacc aatccgaccg
      241 acagcgtatc gactgatttg aagatagaga cgccgatgat caacgtgatc agcaccggaa
      301 gaaggatcat gaagaagagg gcttgccggt cgcgcgtaac tcggcgaagg ctgaggccca
      361 tgatcgtgag gacgctcatg cgaccaccag cttccgggac cgaaatgcgg cgacggtgag
      421 accgaccaca ccgaaggcca ggatcacccc aaccgcgggg aggatcgtac tcaccccgcc
      481 cccatccgcg atcaggtcag tgaatgatcg cagcgcccaa ccgttgggcg tgaggagcga
      541 gagtttccgg aagagttcgg gggcctgatt cagggggatg aagttgccgc ccagcagcgt
      601 cagagccatg gtcgccatag ccccgaaggc gtcagcttgt tccgccgtct tggtgaacga
      661 gatgaccatc gcagttacgc caaccgcggc gatgacgatc gcgacggcga tcgcggcaac
      721 agcgagatga tctcccaggt tggctccgaa gatgaacgtc actgcgacga ggagcgcgcc
      781 aagactcgta agaccaacaa cgaacaccga cagcagcttc ccgagaagga tcgatgtcgt
      841 cgaggttggc gaggcggcaa ggcgcgccag tgttccctcg cgcttctcgg cgatgaggct
      901 tcgcgcgccg aactgaacgg tgaagaagag gaagaagatc gccatcgagg ggccgaagta
      961 tgcggccggt ttgacctcgc gtgttccaac cttgccctgc cgcaactcga tcggcacgcg
     1021 tatgttctga agcagcggca ccagggcacc cacctcttcg ggtgtggcgt tgtcggggtc
     1081 ggggagggcg gtcgccaccg atagccggtt agcgtttatc tgcgagatga aggcgttgga
     1141 gatagcgatc gccacgtcgg ccccgatctg cgatgacgcg cttcgaagaa ccttgaggtt
     1201 ggcgacctgg cccgtgagga tcgactggga gaaccccttg gggatgacga atgccgccga
     1261 tgccgccccg gcatcgagga tgggtcgagc ctgcgcctcc gtcgacttcg ttgaaagcgt
     1321 catgaagtcc ttgagggcgg ggcccttgag cacctggtca acgaaacccg cagcgagggg
     1381 gccgtggtcg agatcaacca cgatgtagtt ggggtggaac gatccaccgt ttccagcgaa
     1441 cgcgaagttg atgatggtgg cgagcgcgag gggagcgatg aatccctgga tgaagaagga
     1501 gcggtcgcgt atgcgcagtt tgatgtcctt gatcgcgatg actatggcgc ccatcagtct
     1561 ctcaacgcct tcccggtcag gtgaaggaag accgcttcga ggtcgggctc gatgacctcg
     1621 gcccccgtaa ccgttacacc tgccgacgcc gcgtgttcga ggatgcgcgg aagtgccgcc
     1681 gagccctgtc cggccaacag tcggatccca tgctcatgtg caacgacttc gctgatccct
     1741 tcgacctgac cgagcgaacg ggccaaagtc tcgatgttgc cagtggcagc gatctcgacg
     1801 cgatctctct gaccgagttg ttcaacgagc tgccgtcgcg tcccttcggc gatgagcttg
     1861 ccttcgtcga tgatgccaac gcggtcgcac aaccgctcca cctcctccat gtagtgcgag
     1921 gtgtaaagga cgctcatccc ctgcgcgttg agggtttcga cgttttcgag gatggcgttt
     1981 cggctctgcg gatcgacacc gaccgttggt tcatcgagaa ccagaagtgc agggttgtgc
     2041 agaagtcccg ccgcgatatt cagtctgcgt ttcatcccgc ccgagtactc ggagacccgg
     2101 tcgcccgcgc gatccgtcag accgacgacc tcgagaacgt gtgcaaccct ggccttcagt
     2161 tctttcccgg agagcgagta gagcctgccc cagaatcgta ggttctcggc ccccgacagg
     2221 tccgggtaga gggcgatctc ttggggaacg tagccgatgg agcggcgtgt ctcggtcgag
     2281 cgtgtggtca tcggctg
//
LOCUS       DATDOZ010000028         5239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3164430, whole genome shotgun sequence.
ACCESSION   DATDOZ010000028 DATDOZ010000000
VERSION     DATDOZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3164430"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..898
                     /locus_tag="VI541_00500"
     CDS             146..898
                     /locus_tag="VI541_00500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLF69419.1"
                     /translation="MLPVTGLIPIHDANPTRTRSILTIALIIVNVAIFFVEPQLGSGS
                     SCQLGVFLFHWGVVPKELVSGKALSGNICAGFPLQDKSIYLSLITSMFLHAGWLHLAG
                     NMLFLWVFGNNVEDRLGKIRFGIFYLACGIAAGLAHTFVNPESVTPTIGASGAIAGLL
                     GAYIVLFPKARVTTIIPLGFLFIRDLPAYLVLGFWFLSQFFIARGQQAGGGVAWMAHV
                     GGFVAGIALIVLLGGLKSRSTLSDGPPDPIWS"
     gene            complement(858..1709)
                     /locus_tag="VI541_00505"
     CDS             complement(858..1709)
                     /locus_tag="VI541_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HLF69420.1"
                     /translation="MLAYARRWDSSDMSLVPLSPIEAGELLSRYRGFAVDLDGVMWRG
                     DEPVMGAASAIAQMRTHGARVVFLTNNGGSSPAEFARRLTDAGVPADGADVITSVTVA
                     REWTEAAGLERPRIVVLGAPALCEQVEDFASVVAFDDGSPDVVLLGRDTDFDFETLDR
                     ASRAIRDGAAFLALNLDATMPIEGGLAPGTGALAAALIVASGREPIVMGKPQFAMMQC
                     ARRILGTNEALMIGDRLDSDIAGAIQIGWDAALVLTGVTHHDDELVPEPTYLLNSISD
                     LGAHQRE"
     gene            1724..2872
                     /locus_tag="VI541_00510"
     CDS             1724..2872
                     /locus_tag="VI541_00510"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HLF69421.1"
                     /translation="MPRLDPFTAIRYSPADPVERVSAPPYDVISPESRDVLAADPHNV
                     VHLTLPQDEGPRDRYSVAATTLAGWLEQGVLVPDAEPSFFVYGTDETEGVSHSSRGVI
                     GALTLEEFGTGVHPHERTLAGPKADRLALMQATDANLEPLWFFGSGPLRGFEEAVVAA
                     TSADPLSDLTTEGVRHRLWRLLPEDAQLLLTDLRTTDLIVADGHHRYETALTFRGLRR
                     ATRGPGPWDQTLALISDTFYMPPQLRPIHRVCRGDVTGVLAELAIEELGLELSALLEE
                     VGRRGKGSIGVISSRVSGIFRSGGDLDTEYLGGIVEKHSAPVRYEHAFEQLSVEIGDG
                     ATVFIMAPVELPTVAVRAISGERMPPKTTLFMPKPRSGLVMRSLVGSS"
     gene            2935..4074
                     /locus_tag="VI541_00515"
     CDS             2935..4074
                     /locus_tag="VI541_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69422.1"
                     /translation="MTRSTRLTKRRLWYMGLALAWSLFIALGSQGSTPSKTINDLRPS
                     GQGWYWDRPAQSDDLFNPGLPSGGGNAATAADHLYVGWDGVNQRENMISGINFDLFQA
                     GVPEGSKIVGFVITAIEHPAGENQSLNAAEAGKQGVMVCAMPQFVAGEAAANINNAPP
                     RDCTVSVASLRPSGAVDPSATGAAITYAWSFDVTSLAAEQAQSGSQVAFSLQPAITTK
                     SAQTWFTTFHSGDYSEKTTAADGTSVDVPKPGILVKVTYLPPVTEPEPDTSTSTGSAF
                     DIGGFSDGPSFSDTSGSLAETLAPEPPPTVNTRRSNTQIPVAGVFEGKPAGFWDLPPI
                     AWVSGILGLALLMASGWALNSDPMQGRPPGAASSLMAKLDDENGG"
     gene            4078..>5239
                     /locus_tag="VI541_00520"
     CDS             4078..>5239
                     /locus_tag="VI541_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69423.1"
                     /translation="MTRSWRAVCAVVALALLLSACGQKPGVHSVGGAARFASATEGND
                     LDVAGAGSGGELAAGVSDGTTSQGTLRNTTSTGGTKTGGRLPTQTQNDPNDRVGITKT
                     TIKIGFHAPLTGAAAIDLNDLRYGNDTYARWLESKGVDIFGRKTQVVFEDDQYSPSVA
                     VAVCRKLVEQDGVFLLLGGAGTDQIQACARYAYSKRIPYLSAGVTEQVVNKLPNYLAF
                     SMSYPAQAAPLAKLIKTFDASAHGGAGGKVLLDRCNSDACAPDPRAPAAAGGSAANST
                     PKVAIIYSNTEGFYDARDAFVRAMGSDTTLISITKYTISSSEASSVVTQLKSAGIDVV
                     YVLTSPTNFGNILSPAEQQGYRPRWVGVGLTMGINLVAAPACGRQRTTFENAI"
BASE COUNT         1006 a         1708 c         1525 g         1000 t
ORIGIN      
        1 cctgcttcac caggtcgcga gccaacctct ccgcctgctt caaggggacc tcggtcaaac
       61 tcgaggccat ctgtatggca gacctgatct gttcgagcat gatttctccg atcactccgc
      121 tggatgcgca ggatatcctg cccccatgct tccggtcacg ggcttgatcc cgatccacga
      181 tgcaaacccg acgcggaccc ggtcgatcct taccatcgcc ctgatcatcg ttaacgtcgc
      241 catcttcttt gtcgaacctc agctgggttc aggatcgagc tgccagctcg gagtcttcct
      301 cttccactgg ggagtggtcc cgaaggaact tgtctccgga aaggctctct cggggaatat
      361 ctgcgccggg ttccccctgc aggacaagtc gatctatctg tctttgatca catcgatgtt
      421 cctgcatgct ggctggctcc acctagctgg gaacatgctc ttcctctggg tcttcggcaa
      481 taacgtcgag gaccggcttg gcaagatccg gttcgggatc ttctacctcg cgtgcggtat
      541 agccgcgggt cttgcccaca cgttcgtaaa tccggaatcg gtcacgccta ccatcggagc
      601 gagtggagcg atcgcgggcc tcctcggcgc gtacatcgtg ctcttcccga aggcccgcgt
      661 cactacgatc atccccctcg gattcctatt catccgagat ctgccggctt acctggtact
      721 tggtttctgg ttcctgtctc agttcttcat cgcacgcggc cagcaggccg gagggggcgt
      781 tgcctggatg gctcatgtcg gggggttcgt cgctggcatc gccctgatcg ttctacttgg
      841 gggactgaaa agtcgttcta ctctctctga tgggccccca gatccgatat ggagttgagt
      901 agataggtcg gttcgggaac aagttcatcg tcgtggtggg ttacccccgt caataccaaa
      961 gcagcatccc agccgatctg gatcgcacct gcgatgtcgg agtcgagccg atccccgatc
     1021 atcagggcct cgttcgttcc gaggatgcgc cgggcacact gcatcatggc gaactggggc
     1081 ttgcccatca cgatcggttc cctgccactc gcgacgatca gcgccgccgc cagggccccc
     1141 gttcctggag cgagcccacc ctcgataggc atggtcgcgt cgaggttcag ggccaggaag
     1201 gccgcgccat cccttatcgc acggctcgct cggtcgaggg tctcgaagtc gaagtctgta
     1261 tcgcgcccga gcagaacgac gtcgggagaa ccatcatcaa acgcaacgac gctggcgaaa
     1321 tcctcaacct gctcacacaa cgcaggagca ccgaggacca cgatgcgcgg acgttccaag
     1381 cccgccgcct ctgtccactc ccgtgcaacg gtgaccgacg tgatgacgtc ggcaccatcg
     1441 gcaggcactc cggcgtctgt cagtcgcctc gcgaactcgg ccggggaaga gcctccattg
     1501 ttggtaagga agacgaccct ggccccatgc gtccgcatct gagcgatcgc tgaagccgca
     1561 cccatgaccg gttcatcacc tcgccacatg acgccgtcga gatcgacagc gaaccctcgg
     1621 tatcgcgaaa gtagctcgcc tgcttcgatc ggcgataacg gaaccagtga catgtcgcta
     1681 ctatcccatc ggcgggcgta ggccagcatg gtataagtgc aacatgcccc ggctcgaccc
     1741 attcaccgcg atccgctact cacccgccga ccccgtggag cgcgtcagcg cgccaccgta
     1801 cgacgtgatc tcgcccgaga gtagagatgt gctcgccgcc gacccccaca acgtcgtcca
     1861 cctcacgctc ccccaggacg aggggcccag ggacaggtac tcagtcgcag ccactaccct
     1921 cgcagggtgg ctcgagcaag gtgtactcgt gccggacgca gagccctcgt tcttcgtcta
     1981 cggcaccgac gaaaccgaag gcgtctcgca ctcatcgcgc ggagtcatcg gcgccctcac
     2041 cctcgaggag ttcggaacag gtgtccatcc ccacgagcga accctggctg ggccgaaggc
     2101 agaccgcctt gccttgatgc aggcaaccga cgccaacctc gagccactct ggttcttcgg
     2161 gtcgggcccc ctaagggggt tcgaagaggc ggtggtcgcg gccacatcgg cagatccgct
     2221 ctcggacctc actaccgaag gggtgcgtca cagactctgg cgactgctac ccgaagatgc
     2281 acaactgctc ctcaccgatc tgcgaaccac cgacctcatc gttgcagacg gacaccaccg
     2341 ttacgagaca gcactcacgt tcaggggtct tcgtcgcgcc acacgggggc cgggcccttg
     2401 ggatcagacc ctcgcgctca tctcagacac tttctacatg cccccgcaac tacggcccat
     2461 ccatagggtc tgtcggggag atgtgactgg ggtcttggct gagctcgcga tcgaggaact
     2521 cggcctcgaa ctatctgcgc tactcgaaga ggttggaagg cgcggcaaag gctcgatcgg
     2581 agtcatctcg tcgcgcgtct cagggatctt tcggtcgggt ggagacctcg acaccgagta
     2641 cctcgggggg atcgtcgaaa agcactcggc acctgttcgc tacgaacatg cattcgaaca
     2701 gctatcggtt gagatcggcg atggcgcgac ggtgttcatc atggctcccg tggaactccc
     2761 cactgtggcg gtcagggcta tctcaggtga gcggatgccc cccaagacca cccttttcat
     2821 gcccaagccg cgaagcgggt tggtgatgcg ttcgctggtt ggttcatcct gaaccccaag
     2881 gccctttggt gggctaagat ggtgactagc tagcatgact cttaagggtg atcgatgact
     2941 aggtcaacac gcctcaccaa gcgtcgcctt tggtacatgg gcctggccct cgcctggagc
     3001 ctcttcatcg ccctagggtc ccaaggctca accccttcaa agacgataaa tgaccttcgt
     3061 ccatccggac agggctggta ctgggatcgg cccgcgcagt ccgacgatct cttcaacccg
     3121 ggtctcccgt ctggcggagg gaacgcggcg accgccgcgg atcatctcta tgtcgggtgg
     3181 gacggtgtga atcaaaggga gaacatgatc tcggggatca acttcgacct cttccaggcc
     3241 ggagtgcccg agggctcaaa gatcgtgggc tttgtgatca cggctatcga gcatcctgcc
     3301 ggcgagaacc agagtctcaa cgccgcagaa gctgggaaac aaggcgttat ggtctgcgcg
     3361 atgccacagt tcgttgcggg agaggccgct gcgaacatca acaacgcacc gccgcgcgac
     3421 tgcaccgtat cggtcgcatc gctccgaccc tcgggcgccg tcgacccaag tgctacgggt
     3481 gcggccatca cctacgcttg gagcttcgat gttacgagcc tggctgcaga gcaggctcag
     3541 tcggggtcgc aggtcgcatt ctcgctgcaa cccgccatca cgacgaagtc cgcccagaca
     3601 tggttcacga cattccacag cggcgactac tccgagaaga ccacggctgc tgacggcact
     3661 tcggtggatg tacccaagcc agggatcctc gtcaaggtga cctacctccc accggttacc
     3721 gaacccgagc cggataccag cacatcgaca ggatccgcat tcgatatagg cggattctcg
     3781 gatggaccca gcttctcgga tacatcaggg agcctggccg aaactttggc ccccgagcca
     3841 cctccaacgg tcaacacccg aaggtcgaat acacagatcc ccgtggcggg ggttttcgaa
     3901 ggcaaaccgg caggtttctg ggatcttccc cccatcgcct gggtatcggg gattcttggg
     3961 ctcgcgcttc tcatggcctc cggttgggcc ctcaactccg atcccatgca agggcgccct
     4021 ccaggggccg ccagttcgtt gatggccaag cttgacgatg agaacggggg atgatcgatg
     4081 actagatcct ggcgtgccgt ctgcgcggtg gtcgcgttgg ccctgctgct ctcggcctgc
     4141 ggacagaagc ccggcgtcca ctctgtgggt ggcgctgcgc gcttcgcatc ggccaccgag
     4201 ggcaacgatc tcgacgtcgc gggggctggt tctggtggcg agttagcggc cggcgtcagc
     4261 gacggcacga cctctcaagg taccctccgc aataccacgt caacgggtgg gaccaagacc
     4321 ggtggcaggc tccccacgca aacacagaac gaccccaacg atcgggtggg catcacgaag
     4381 accacgatca agatcggctt ccacgctccc ctcaccggag cggcggccat cgacctcaac
     4441 gaccttcgct acggcaacga cacctacgct aggtggttgg aaagcaaagg cgtcgacatc
     4501 ttcggtcgca agacccaggt tgtattcgaa gacgatcagt acagcccatc ggtagcggtt
     4561 gcggtctgcc gaaagttggt cgaacaagat ggggtgttcc tcctgcttgg cggcgctggc
     4621 accgaccaga tccaagcctg cgcgcgatac gcgtactcga agaggatccc gtacctctcg
     4681 gcaggagtga ccgaacaggt ggtcaacaaa ctgcccaact atctcgcgtt ctcgatgagc
     4741 tacccggctc aagcagctcc cctggctaaa ctcatcaaga ccttcgatgc ttcggctcat
     4801 ggtggagctg ggggcaaggt gctgctcgac cgctgcaact ccgacgcctg cgctccagat
     4861 ccacgcgcac cagccgcggc tgggggaagc gctgccaact ccacccccaa ggttgcgatc
     4921 atctactcga acaccgaggg cttctatgac gcccgcgatg ccttcgtgag ggcgatgggg
     4981 tccgatacga cactcatctc gatcacgaag tacaccatct cgagttcaga agcatcgagt
     5041 gttgtcacgc agctcaagtc agcagggatc gacgtggttt acgtccttac ctcaccgacc
     5101 aacttcggca acatcctcag cccagccgaa cagcagggat accggccgag gtgggtcggc
     5161 gttggcctta cgatggggat caaccttgtg gccgcgccgg catgtggtcg tcagcgcacg
     5221 accttcgaga acgccatct
//
LOCUS       DATDOZ010000029         7991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2118703, whole genome shotgun sequence.
ACCESSION   DATDOZ010000029 DATDOZ010000000
VERSION     DATDOZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118703"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VI541_00525"
     CDS             complement(<1..296)
                     /locus_tag="VI541_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69424.1"
                     /translation="MRRGSSLSAVAFMVAALAGSLWAGPTQAAGHTVTLNSDSGSYFA
                     PDLTEAVSGDTVTFHWNSGFHTATWYGGDQGDPASFDSEGKSAPTSDYTITFLGG"
     gene            479..1279
                     /gene="whiG"
                     /locus_tag="VI541_00530"
     CDS             479..1279
                     /gene="whiG"
                     /locus_tag="VI541_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HLF69425.1"
                     /translation="MQQQTDDAVVIEEDPILAIWRDFKATGDAAARDNLIVHYSPLVK
                     YVAGRVSTGLPSNIEQADLVSYGIFGLIDAISKFEIDRGIKFETYAINRIKGAIIDEL
                     RSIDWVPRSVRTKAREVERTLAKLESELRRAPTDQEVAAAMGVSVEDLRQVYTQMSFV
                     SVAALDELMSVGGEKGDSVSLIDTLEDRNAESPEASFEVEEMKSILAGAINKLSEREK
                     IVVTLYYYEGLTLAQIGRVLGVTESRICQMHTKAVLQLRSRLSEARAD"
     gene            1422..2387
                     /gene="rpsB"
                     /locus_tag="VI541_00535"
     CDS             1422..2387
                     /gene="rpsB"
                     /locus_tag="VI541_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLF69426.1"
                     /translation="MAVVTLKDLLEAGVHFGHQTRRWNPKMRRFIFTERNGIYIIDLL
                     KTMAGIDDAYRFCRSLAARGGTVLFVGTKKQIADSIAEEASRVGMPYVNYRWLGGMLT
                     NFQTMHRRILRMRELEQQESQGIFEEIPKKEALKLRNELEKLRKNLDGIRDLNRRPDA
                     VFILDTRKEDICVREARKLGMPIIAVVDTNCDPDEVDYIIPGNDDAIRSGGLLTRVIA
                     DAIAEGLTKRPPEVIAAAEAAAASGITLTAGGEEPPAEWEIMLAQQETDIAKRSKDEL
                     VPEAGPAASTEDKFGKLLDATPLPGEEIEEELKAIYKDDDEGKHR"
     gene            2384..3010
                     /gene="tsf"
                     /locus_tag="VI541_00540"
     CDS             2384..3010
                     /gene="tsf"
                     /locus_tag="VI541_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLF69427.1"
                     /translation="MSINAQDVARLRSETGAGMMDCKKALTETAGDFEAAKDLLRTWG
                     LAGVEKRAGRAASEGMVGYYVHQLDADLPPKVGVLVELNCETDFVAKTPDFKQLARDI
                     AMHIAAMEPRWASRTEIPDDLIDRERKIVLESDSVKGKPENIVEKIVEGKLTNLYSGK
                     GGVLVDQVYVKDESGRKTVGELISEYAAQVKENIVVRRFARFRVGEDD"
     gene            3012..3737
                     /gene="pyrH"
                     /locus_tag="VI541_00545"
     CDS             3012..3737
                     /gene="pyrH"
                     /locus_tag="VI541_00545"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLF69428.1"
                     /translation="MGRGPFRRVLLKLSGESFAEPTLGFGIDANIVSSLAKQLASVQS
                     SGVQIGVVVGGGNIFRGSQAAAMGMERARADYMGMLATVINALALQDALGQVGAECRV
                     LSAIWMQEVAEPFIRGRALRHLERGMIVIFAGGTGNPYFSTDTAASVRALEVGAEALL
                     KGTKVDGIYDTDPVNNADAVRFEHLEYIEVLNKGLKVMDSTAISLCMDNGLPIVVFDL
                     TRDGNIARVVAGENVGTIVSAGA"
     gene            3738..4295
                     /gene="frr"
                     /locus_tag="VI541_00550"
     CDS             3738..4295
                     /gene="frr"
                     /locus_tag="VI541_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017151256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLF69429.1"
                     /translation="MLEDHMKTAQEKMRKAIAASKDEFATIRTGRASPNLLERIEVDY
                     YGTKTPLMQIAGVSVPEARLLVISPYDRSALSAIEKAILGSDLGLTPSNDGSVIRLSF
                     PPLTEERRKELIKQVRERAEEGRVAIRNVRRHSKDEMERIAKEGHVSEDDVKRTEKDL
                     QKITDHFIGEIDELVSHKEKELLEI"
     gene            4308..5195
                     /locus_tag="VI541_00555"
     CDS             4308..5195
                     /locus_tag="VI541_00555"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLF69430.1"
                     /translation="MNSEEDSTEDPETPPESSTRRVGRDLRLATVTGLTLAAMVIGLL
                     FLGPEAFFALAFVVILIAQAEFYMAVRRAGHEPATTLGVVAGAAMLLGVFNRGEAAAG
                     VVLFLTMLFGFLWYLSMHRRTNIVSDLAITVLGVAYVPMLGSFAGLLTKACQPGAHCP
                     PSGGGIALTMIGAAAIYDIAAFAGGSLIGKTPLVPSISPRKTREGALVATIVSLVAIT
                     TLAPVFGPWDHLQALVLAVLVSVAAPLGDMVESMIKRDLGIKDMGGIFPGHGGALDRI
                     DAILFVAPAVYVSLKIFGL"
     gene            5232..6386
                     /gene="dxr"
                     /locus_tag="VI541_00560"
     CDS             5232..6386
                     /gene="dxr"
                     /locus_tag="VI541_00560"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLF69431.1"
                     /translation="MKDLFGSSEQTRIAICGSTGSIGKQALDVVRRFPDRFEIVALTA
                     GTDALTLMEQAREFGPQILGLREGQLPDVDARSFVGEDAAATVARECDASVILNAVVG
                     AAGLSTTMETILSGKTLALANKESLVAGGEAVMAKAGPQQIRPVDSEHSAIWQLLDRL
                     DPAGVRKMILTASGGPFRGWTRAQLEGVTVEQALAHPVWRMGDKITVDSATLMNKGLE
                     VIEAHYLFGFTYDEIEVLVHPQSIIHGMVESVDGAVYAHAAKPDMRLPIEVALAWPER
                     LSPPMVERIEWSALSALTFEPPDAEAFPCLPLAVEAGRRGDSYPAALNAANEVAVRAF
                     LAGALSFVDIPVVIEKVMERHEPVPPTIDAVLEVDAWARDLASSIVKESA"
     gene            6383..7537
                     /locus_tag="VI541_00565"
     CDS             6383..7537
                     /locus_tag="VI541_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HLF69432.1"
                     /translation="MTSSGGVIAFILTLVVMVIVHEFGHYITAKRFGMRVEEYFFGFG
                     PRIFSRRIGETEYGIKAIPLGGYVRISGMNPWQQIPDEEIPRTFGAKPSWQRAIVLGA
                     GSFTHFVLAFLIFTAMYGVVGLHDPDHPTTKIERISETVDGQPGPAKVAGLQQGDKVI
                     AVNARAANTWDKVRAEIRKSPGVPITLDVLRDGKTVSVSVTPLGVKVPVDEAHPARKQ
                     TVGQIGVVPTLPVKKESLPRAVVLGAQTTGVAIARSVVGIGSIFSPHGISRVFGALGG
                     SGRRSADQPFGLVGSARLAGEVASAGEFETLVLFLTGFIVFVGVINLVPLPPLDGGHL
                     LVLLIEKVRRRKVDMRKVVPVAGVVLSFLVILTVVLLYLDIVRPVSNPFQ"
     gene            7564..>7991
                     /locus_tag="VI541_00570"
     CDS             7564..>7991
                     /locus_tag="VI541_00570"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986091.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLF69433.1"
                     /translation="MALTKPSEDFPAWYQEVVKGAEMAEPGLVRGTMIIRPYGYAIWE
                     AIQSGLDRRIKKTGHSNLYFPMFIPMKLIDKEAEHVEGFAPQIAVVTHAGGAALEEPI
                     AVRPTSETIIWATYARWIQSYRDLPLLYNQWANVVRWELRT"
BASE COUNT         1580 a         2389 c         2503 g         1519 t
ORIGIN      
        1 ccgccaagga aggttatggt gtagtcgcta gtgggtgcgc tcttcccttc actgtcgaag
       61 ctcgctgggt ccccctggtc gccgccatac caggtggcgg tgtggaatcc tgagttccag
      121 tggaaggtca cggtgtcgcc cgatacagct tcggtcagat cgggcgcgaa gtaactcccg
      181 ctatcactat tgagggtgac ggtgtggcct gcggcctggg tcggccctgc ccacaaacta
      241 cctgccaacg ctgcgaccat gaatgcaacc gccgatagag atgatcctct tctcacgatg
      301 ccctccgggt ccgcggtccg aacgcgattg cggacatact cagatattcg accctcgcgg
      361 cgtatttcct gcttatccgc tcaacaaggt tccgccggct aacgtgtctt cccgttctat
      421 actcgggctc catcctcgaa cataggatca agccttggcc gccgacggtg ttccaaaagt
      481 gcagcagcag accgatgacg ctgtcgtcat cgaagaggac cccatcctgg ccatctggcg
      541 cgacttcaaa gccacgggcg acgctgctgc ccgcgacaac ctcatcgtcc actactcgcc
      601 cctggtcaag tacgtggctg gccgtgtctc gaccggtctt ccctcgaata tcgaacaggc
      661 cgacctcgtc tcctatggga tcttcgggct catcgacgcg atctccaagt tcgagatcga
      721 cagagggatc aagttcgaga cctacgcgat caatcggata aagggagcga tcatcgacga
      781 actcagatcg atcgactggg tcccgcggtc ggtgcgcacc aaagccagag aagtcgagag
      841 gaccctggca aagctggagt ctgaacttag gcgggccccc accgaccagg aagtggccgc
      901 tgcgatgggt gtcagcgtcg aggatctgcg ccaggtttac acacagatgt cgttcgtttc
      961 ggtagcggca ctcgacgagt tgatgtcggt gggcggcgag aagggcgact cggtgtcgct
     1021 catcgacacc ctcgaggacc gaaacgccga gagtcccgag gctagcttcg aggtcgagga
     1081 gatgaagtcg atcctggccg gggcgatcaa caagctctcc gagcgcgaga agatagtcgt
     1141 cacgctctac tactacgagg gtctgaccct ggcgcagatc ggccgggtcc tcggcgtgac
     1201 cgaatcacgg atctgccaga tgcacacgaa agccgtgctt caactgcgct ctcgtctcag
     1261 cgaagcaagg gccgactaaa ccgactatca tcttccgggc gtgccagccg gctcgcttta
     1321 tcgaatcaag catgttcgcc gacaacggtg cctgatggtc ttcgacctag ggtggggcgg
     1381 atgttagggg agatgactcc cgtaaccgga aaggaggccg catggcggtc gtaactttga
     1441 aggacctgct cgaggcgggg gtccacttcg gacaccagac ccgtcgttgg aaccccaaga
     1501 tgcgtcgttt catcttcacc gagcgcaacg gcatctacat aatcgatctt ctcaagacga
     1561 tggctgggat cgacgacgcc taccgattct gccgaagcct tgcggcgcgc gggggcacgg
     1621 tcctcttcgt cggaaccaag aagcagatcg ctgactccat cgccgaagag gcgagtcgtg
     1681 tcggtatgcc ctatgtcaac tatcgctggc ttggtgggat gctgaccaac ttccagacga
     1741 tgcaccgacg gatccttcgg atgcgcgagt tggaacagca ggagtcgcaa gggatcttcg
     1801 aggagatccc caagaaggaa gcactcaaac tccgcaacga attggagaag ctgcgtaaga
     1861 acctagacgg gatcagggac ctcaatcgcc gacccgacgc ggtattcatc ctcgacacgc
     1921 gcaaggagga catctgcgtg cgtgaagctc gtaagctcgg gatgcctatc atcgcggtcg
     1981 tcgacaccaa ctgtgacccc gatgaagtcg actacatcat ccccggtaat gacgatgcga
     2041 tccggtcggg cggtctgctc accagggtca tcgcagatgc catcgccgag gggttgacga
     2101 agcggccccc agaagtgatc gctgccgccg aggccgccgc ggcttcgggt atcaccctga
     2161 ccgccggtgg cgaagagccg cccgctgagt gggagatcat gctcgcccag caggagacag
     2221 acatcgccaa gcgctccaaa gacgaactgg ttcccgaggc gggccccgct gcgtcgaccg
     2281 aggacaagtt cggaaaacta ctcgacgcga caccgcttcc cggcgaggag atcgaggaag
     2341 agttgaaggc tatctataag gatgacgacg aggggaaaca tcgatgagca tcaatgcaca
     2401 ggacgtagcg cgtctccgct ccgagaccgg cgcggggatg atggattgca agaaggcttt
     2461 aacagagacg gccggtgact tcgaggctgc aaaggatctg ctccgaactt gggggcttgc
     2521 cggagtcgag aaacgggcag gcagggcggc ctccgagggg atggtcggct actacgtcca
     2581 ccagttggac gcagacctgc cgccgaaggt cggagttctg gtcgaactca actgtgagac
     2641 cgacttcgtt gcgaagacgc cagacttcaa acagttggcg cgcgacatag cgatgcacat
     2701 cgcagcgatg gagcctcgtt gggcttcgcg taccgagatc cccgacgacc tgatcgaccg
     2761 agagcgcaag atcgttctcg agtcagactc ggtcaagggc aagcccgaga acatcgtcga
     2821 gaagatagtc gagggcaaac tcaccaatct ctattcgggg aagggcggcg ttctcgtcga
     2881 tcaggtctac gtcaaggatg aatccggacg caagaccgtc ggcgaactga tcagcgagta
     2941 cgcggcgcag gtcaaggaga acatcgttgt tcgacggttc gctcgattcc gcgtcgggga
     3001 agacgactag cgtgggccgc ggcccctttc gcagggtcct cctgaagctc tcgggagagt
     3061 ctttcgccga gccgacgttg ggtttcggga tcgatgccaa catcgtttcg tcactcgcca
     3121 aacagttggc cagcgtccag agctccggtg tccagatcgg agtcgtggtc ggcggcggca
     3181 acatcttccg cggctcccaa gccgctgcga tgggcatgga gcgcgccaga gccgactaca
     3241 tggggatgtt ggcgaccgtc atcaatgccc tggccctgca ggacgctctg gggcaggtgg
     3301 gggccgagtg cagagtcttg agcgctatct ggatgcaaga ggtcgccgaa ccattcatac
     3361 gcgggcgagc gcttcgccat ctcgagcgcg gcatgatcgt catattcgct ggtgggaccg
     3421 gcaaccctta cttctcaacg gatactgcgg cgtcggttcg ggccctcgag gtgggggccg
     3481 aggcactcct caaggggacc aaggtcgatg gcatctacga taccgaccca gtcaacaacg
     3541 ccgatgccgt taggttcgag cacctggaat acatcgaggt cctcaacaag gggctcaagg
     3601 tgatggactc gacggctatc tcgctctgca tggacaatgg actccccatc gtagtattcg
     3661 acctcacccg ggatggcaac atcgcgcggg tcgttgccgg cgagaacgtg ggcaccatcg
     3721 tctcggcggg ggcctagatg ctagaagatc acatgaagac ggcgcaagag aagatgcgca
     3781 aggccatcgc cgcttcaaag gatgagttcg cgacgatccg taccgggcgc gcctcgccga
     3841 atcttctgga gaggatcgaa gtcgactact acggaacgaa gacgccactc atgcagatcg
     3901 caggtgtctc agttcccgag gcccggctgt tagttatcag tccgtacgac cgctcggccc
     3961 tctcggctat cgagaaggcg atcctggggt cagacctcgg cctgactccg tccaatgacg
     4021 ggtcggtcat acggctctca ttcccgccgc tgaccgagga gcgccgcaag gagttgatca
     4081 agcaggtgcg cgaacgcgct gaggaagggc gagtggccat ccgcaacgtc cggcggcact
     4141 cgaaggacga gatggagcgc atcgctaaag agggccacgt ttctgaagac gacgtgaagc
     4201 gcaccgagaa ggaccttcag aagatcaccg accatttcat cggcgagatc gacgagttgg
     4261 tatcgcataa agagaaggaa ctgctcgaga tctgaggacc ggaatctttg aatagcgagg
     4321 aagactcaac cgaagatccc gagactccgc ccgagagttc cacgcgacgg gtcggccgtg
     4381 atctcagatt ggccaccgtc accggtctga cccttgcggc gatggtcatc ggcctcctat
     4441 tcctggggcc cgaggctttc ttcgccctcg cattcgtcgt gatactcatc gcacaagccg
     4501 agttctatat ggcggttcgt cgcgcgggtc acgaacctgc tacgaccctc ggcgttgtgg
     4561 cgggggcggc catgcttctg ggcgtcttca atcgcggtga ggccgcagcc ggagtggttc
     4621 tgtttctcac gatgctgttt ggattcctct ggtacctctc gatgcatcgc cgaacgaaca
     4681 tcgtgagcga cctcgcgatc accgtgctcg gcgtcgccta cgttccgatg ctcggctcat
     4741 tcgcggggct gctcaccaag gcgtgtcagc caggagccca ctgtccgccg agtggcggtg
     4801 gtatcgctct cacgatgatc ggggccgcgg cgatctacga catcgcagcg ttcgctggcg
     4861 gttccctcat cggcaagaca ccgctcgtgc cgagcatctc tccgcgcaag acgcgagagg
     4921 gggccttggt cgcgaccatc gtgagcttgg ttgcgatcac gacgctcgct ccagtgttcg
     4981 gaccttggga tcacctccag gccctcgtgc tcgccgttct cgtgtcggtg gccgcaccgc
     5041 ttggcgatat ggtcgagtcg atgatcaagc gagaccttgg gatcaaggac atgggtggca
     5101 tcttccctgg ccacggcggg gccctcgacc gcatcgatgc catcctcttc gtcgccccgg
     5161 ccgtctatgt ctctctcaag atttttggcc tgtgaatcat cctgtcggcc actttgagac
     5221 atgatgttgg gatgaaggat ctcttcggct cgtccgagca gacgcgtatc gcaatctgcg
     5281 ggtcgaccgg atcgataggg aaacaggctc tcgatgtcgt gcgccgtttc cccgatcgct
     5341 tcgagatcgt tgcgctgacc gcaggcaccg atgccctgac tttgatggaa caagcgcgcg
     5401 agttcggccc ccagatcctc ggccttcgcg aaggccaact acccgacgtt gacgcgagaa
     5461 gtttcgtcgg agaagacgct gctgctacgg tggcgcgcga gtgtgacgca agcgtgatcc
     5521 tcaatgcggt ggtgggcgcg gccggactca gcaccacgat ggagacgatc ctgtcgggca
     5581 agaccctggc actagcgaac aaggagagcc tggtggctgg gggagaggct gtaatggcca
     5641 aggcgggccc ccagcagatc cgcccggtag acagcgaaca ctctgcgatc tggcaactgc
     5701 tagatcggct cgatccagcc ggggttagaa agatgatcct cacggcgagc ggggggccct
     5761 tcagaggttg gacgcgtgcc cagcttgaag gagtcaccgt cgaacaggca cttgcgcacc
     5821 cggtgtggag aatgggagac aagatcaccg ttgactcggc gacgctcatg aataaggggc
     5881 tcgaagtgat cgaggcccac tacctgttcg ggttcaccta cgacgagatc gaggtgctgg
     5941 tccacccgca gagcatcatc cacggcatgg tcgagtcggt cgatggcgcg gtctacgcgc
     6001 acgcggccaa accggacatg cgtcttccga tcgaggtcgc tctcgcgtgg ccagagcggt
     6061 tgagtccgcc gatggttgaa cgcatcgaat ggtcggccct ctctgcgttg accttcgagc
     6121 ctccagacgc cgaagccttc ccatgcctac ctctcgccgt cgaggcgggg cgccgcggcg
     6181 acagctaccc cgctgccctc aatgctgcga atgaggtggc cgtgcgtgcg ttcctcgccg
     6241 gggctctgag tttcgtggat atcccagtcg tgatagagaa ggtcatggag agacacgaac
     6301 ccgtaccccc gaccatcgat gccgttctcg aagtagacgc gtgggcccgc gatcttgctt
     6361 ccagcatcgt taaggagagc gcgtgacctc cagcggcgga gtgatcgcat tcatcctgac
     6421 cctcgtggtc atggtcatcg tccacgagtt cgggcactac atcacggcca aacgattcgg
     6481 gatgcgggtc gaggagtact tcttcggttt cggcccacgc atcttctcac gccgtatcgg
     6541 cgagaccgag tacgggatca aggccatccc gctcggggga tacgttcgca tatcagggat
     6601 gaacccatgg cagcagatcc ccgacgagga gatccccaga accttcggtg ccaagccctc
     6661 ctggcagcgc gcgatcgttc tcggcgcagg ttccttcact catttcgttc ttgctttcct
     6721 gatcttcact gcgatgtatg gggtcgtggg tctgcatgat cccgatcacc cgactaccaa
     6781 gatcgagagg atctcggaaa cggtcgatgg gcagcctggc cctgccaagg ttgcaggtct
     6841 gcagcagggc gacaaggtca tcgcggtgaa cgccagagct gcgaacactt gggacaaagt
     6901 ccgcgccgag atacgcaaga gtccaggcgt gccgataact ctcgatgtcc tgcgcgacgg
     6961 caagaccgtg agcgtctcgg tgacgccact tggggtgaag gtgcccgtcg atgaggcgca
     7021 cccggcgcgc aagcagacag tgggtcagat cggggtcgtc cccaccctcc ccgtgaagaa
     7081 ggagtccctg cctcgggcgg ttgtgttggg ggcccagacg accggcgtag cgatagcccg
     7141 aagtgtcgtc ggtatcgggt cgatcttctc tccgcacggg atctctcgtg tgttcggtgc
     7201 actcggcggg tcgggtcgtc gaagcgccga tcaacccttc ggcctcgttg ggagcgcgcg
     7261 gttggcgggc gaggtggcat ccgccggaga gttcgagaca ctggtcctct tcctcacggg
     7321 gttcatcgtc ttcgtaggag tgatcaacct cgtccccctg cctcccctcg atggtggtca
     7381 cctgttggtg cttctcatcg agaaggtgag gcgtcgcaag gtcgacatgc gaaaggtcgt
     7441 gccggttgcg ggagtagtgc tcagttttct ggtgatcctc acggtcgtgc tgctctacct
     7501 cgacatagtc agacccgtct ccaacccgtt ccagtgagcc acgccgttta gacttgccgg
     7561 actatggcgc taaccaaacc gagcgaagac tttccggcct ggtatcagga ggtcgtgaag
     7621 ggggccgaga tggccgagcc tggtctcgtg cgcggcacga tgatcatccg gccttacggc
     7681 tacgcgatat gggaggcgat ccagtccggc ctcgatcgca ggatcaagaa gaccggacac
     7741 tcgaacctct acttcccgat gttcatccct atgaagctca tcgacaagga agcagaacat
     7801 gtcgaaggct tcgcgccaca gatcgcggtg gtgacccacg cgggaggcgc cgctcttgag
     7861 gagcccatcg cggtgcgtcc gacatccgag acgatcatct gggcgacata cgcgcgctgg
     7921 atccagagct atcgtgacct gccactcctc tacaaccagt gggccaacgt ggttcggtgg
     7981 gagttgcgga c
//
LOCUS       DATDOZ010000030         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2119310, whole genome shotgun sequence.
ACCESSION   DATDOZ010000030 DATDOZ010000000
VERSION     DATDOZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119310"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..721
                     /gene="pgsA"
                     /locus_tag="VI541_00575"
     CDS             161..721
                     /gene="pgsA"
                     /locus_tag="VI541_00575"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLF69434.1"
                     /translation="MRRRFGWANAVTVLRIGLVPLFAWLLLAPQNPASAVNWGAFVVF
                     AVAAWTDTLDGYLARRQGMITPLGQFLDPLADKLLVGVALLGLVLIRDFPLWAALVII
                     GREFAISILRTAGLRRGRSMPATIHGKIKTALQVPMVLIWLMPRRGTMLTVQDVVMWL
                     AVAFTVISGAQYLMRARGLLDRSQPS"
     gene            718..1968
                     /locus_tag="VI541_00580"
     CDS             718..1968
                     /locus_tag="VI541_00580"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HLF69435.1"
                     /translation="MRAQIISVGTELLLGQIANTNAQVMSQWLAEVGVDVLFHVTVGD
                     NEERLAGSLTEALQRSDVVIVTGGLGPTHDDLTREAISRATGRALIRDEQIELWLREL
                     FMKLGREMPEMNLRQADVPIGAVSIPNPLGTAPGVDLTIDGTRIFAVPGVPAEMESMM
                     RRWVLPALSKVTGAVIKSRIVRVVGLGESDVARRVSAIIEGLEGAGGATIAILASAGE
                     VRLRITAKAPDEAAALMQIAPIEQELRTTLGRAVVGADDEVIEIVIGETMRSRGLTLA
                     VAESFTGGMVASTLIGAPGASRFLEAGYICYSDEAKIRDVGVAPALIESDGSVSAAVA
                     EAMASGARERADTDLGIATTGEAGPVASSDVQGHAFIALAWEGGSTARELRLPGDREA
                     IRKRGSLAALDLLRLWLNGEISEK"
     gene            2142..2489
                     /locus_tag="VI541_00585"
     CDS             2142..2489
                     /locus_tag="VI541_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69436.1"
                     /translation="MNGTLESSEPETWLAALANDNFPGRLQSCVVAFIPFGGLYLGTA
                     FLLDRFTVIPWMIIQWTFTLAYVSTGAILLGIAVSPLAFKGRLRVAAAAGAVAVALTA
                     VATTVIYLIKGGT"
BASE COUNT          438 a          742 c          848 g          525 t
ORIGIN      
        1 caccttcacg gccgtagatc atctacgcgt tacgatcggg gatctcggtg cagtgtcgtt
       61 gaccttgaac ggtcgcaacc tcggcgttgc gggtagcgac ggtgaggcaa ggaccctgac
      121 cttcacgcgg gatggcggcc ccatcggtag gggttgaatc atccgacgca ggttcggctg
      181 ggctaacgcg gtaacggtcc ttcggatagg gctcgtcccg ctgttcgctt ggctcctgct
      241 cgccccccaa aacccagctt ccgctgtcaa ttggggcgca ttcgttgtct tcgcggtcgc
      301 agcctggacg gacacgctcg atggatatct cgctaggcgt caggggatga tcacacccct
      361 cgggcagttc ctcgaccctc tcgctgacaa gctcttggtg ggggtggccc tgctggggct
      421 tgtcctcatc agagactttc ccctctgggc ggcgctggtc atcatcggtc gtgagtttgc
      481 catctcgatc cttcgcaccg ccggcctccg caggggtcgc tcgatgcccg cgaccatcca
      541 cggaaagatc aagaccgcac ttcaggtgcc gatggtcttg atctggttga tgccgcgacg
      601 cggcacgatg cttaccgtac aggatgtggt gatgtggctt gcggtcgcgt tcactgtcat
      661 ctcgggggcc cagtatctga tgcgagcgcg agggctcctc gataggagtc agcccagttg
      721 agggcgcaga tcatctcggt ggggaccgag ttgcttctcg ggcagatcgc caacaccaac
      781 gcgcaggtga tgtcgcagtg gctggccgag gtgggtgtcg atgttctctt ccatgtaacc
      841 gtcggtgaca atgaagaacg gttggctggc tcgctgactg aggcgctgca gcgaagcgat
      901 gtcgtcatcg tcacgggtgg gttgggcccg actcacgacg acctgactcg cgaggctatc
      961 tcccgcgcga ctggccgcgc gctcatccgc gacgagcaga tcgagctctg gctgcgcgaa
     1021 ctcttcatga agctcggccg cgagatgccg gagatgaatc ttcgtcaggc cgacgttcct
     1081 atcggcgcgg tatcgatacc caatccgctg gggacggccc ccggcgtcga cctcacaatc
     1141 gatggcacgc gcatattcgc tgttcctgga gttccagccg agatggagtc gatgatgagg
     1201 cgctgggtgc tgcccgctct ttcaaaagtc acgggcgcgg tcatcaagtc gcgcatcgtg
     1261 agggtggttg gtctgggaga gtcagatgtg gcgcgtcgag tgagcgcgat catcgagggc
     1321 ctggaggggg ccgggggagc gaccatcgcg atcctggcct cggcgggtga ggtgagactc
     1381 cggataactg cgaaggcccc cgatgaggcc gcggctctca tgcagatcgc accgatcgag
     1441 caggagcttc gaacgaccct cgggcgtgcc gtggtagggg ccgacgatga ggtgatcgag
     1501 atagtgatcg gcgagacgat gcgttctcgc ggactcacgt tagcggtggc cgagtccttc
     1561 acagggggca tggtcgcgtc gacgttgatc ggcgctccgg gggcctcgcg gtttcttgag
     1621 gccggctata tctgctactc cgatgaagcg aagatccgtg atgtgggagt ggcgccagcc
     1681 ttgatcgagt cggacggatc ggtcagcgcg gcggttgccg aggcgatggc atcgggtgca
     1741 cgcgagcgtg ccgataccga ccttgggatc gcaaccacgg gcgaggccgg gccggtcgca
     1801 tcatccgacg tccagggtca cgcattcatt gcgcttgcgt gggagggcgg atcgactgcg
     1861 cgtgagctga ggcttccagg agatagggag gcaatcagaa agcgtggctc cctggcggct
     1921 ctcgacctgc tgcgcctctg gctgaacggc gagatcagtg agaagtgagc tttatactga
     1981 cccgctgggc gggctaacac gcatgggggc cccgggatga atcgagcaat tgcgctgctt
     2041 aacctggggg cctgccgtcg ggtccatcca aggccacgcg cgttaaccgg accgagtcgg
     2101 gcgtttaacc aggtccgcag aagcatgagt taaacagact gatgaatggg acacttgagt
     2161 cttccgaacc agaaacatgg ctagctgccc tcgcaaacga caatttccca ggtcgtctgc
     2221 aatcctgcgt cgtcgcgttc ataccgtttg gtggtcttta tttggggacg gcctttttgc
     2281 tcgacaggtt tacagtgatt ccatggatga tcatccaatg gacgttcact ctcgcttacg
     2341 tctctacagg ggcgatcctc ctggggattg cggttagtcc cctcgccttc aagggtcgcc
     2401 ttcgagttgc cgcagcagcc ggagcggtag ccgttgccct aacagctgtg gccaccaccg
     2461 tgatttacct catcaaaggc gggacgtgat ctactgctgt ttaacacggc cgtgcaaccg
     2521 accgccttcg gcggcggctg acggcccaag ccg
//
LOCUS       DATDOZ010000031         3342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2376690, whole genome shotgun sequence.
ACCESSION   DATDOZ010000031 DATDOZ010000000
VERSION     DATDOZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376690"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1466
                     /locus_tag="VI541_00590"
     CDS             144..1466
                     /locus_tag="VI541_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF69437.1"
                     /translation="MREISLAGRYRLVEVLSRQENIVSALATDEVLGRSVMARILKQG
                     VPEELISTFRRSALAWAALTHPNIATVYDAGRHRGLEYLVSEFVPRGTLADKLAAGPL
                     DPMRVCEIGDQLCAALSHASAAGVGHGHITADNVLITESGQVRLTDFGVHAALGGVVP
                     NAADDIRDVGQILFRALTGRALDECPTNSPRDQRPDVPRFLDEAIVRATSEEGYQDAA
                     AFAAALGVAASLPAPARAAPSVTEPPGEQASFLRSEARLFLTIALIVAVAAAIVLAIP
                     TITRNIAPTAQTRATPVVLGAVGSGIYDPPPGDGHENDELVSLAFDGDRRSAWRTDRY
                     GNASFGSLKSGVGIWVDLGKPRRITSVIIHTGHPGWLGTLRYSEDSVIWTDPGASEEV
                     EDGHAFSVNQTHRYVMLWIARVVPVEPADKNPFQADVTEFRALGGGAT"
     gene            1463..2041
                     /locus_tag="VI541_00595"
     CDS             1463..2041
                     /locus_tag="VI541_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF69438.1"
                     /translation="MSEQISENAADLALVKAFVSDHDIDALEQLLEKHRNRIFTHAFR
                     VTRNRSDALDVTQEVFITVYKKSSSFRGASSFDTWLYRLTTNACYDLLRKRARQPVAS
                     AEIPEGSGRVDDEIARVDDRLAVEAALASLSPEHRAALVMRELEDMTYEQIAEVLEVP
                     VGTVKSRIARGRAALADLVTEHQSQPGRQRDE"
     gene            2043..2768
                     /locus_tag="VI541_00600"
     CDS             2043..2768
                     /locus_tag="VI541_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69439.1"
                     /translation="MHEHLDDEALSGLLDTDGIETISSCPRCSGRYRAMLSASTILRE
                     IDPAPMTADEVRELRLGILAATARRPPARWIAAAAGSVAILVAVGLYISGPRPRNDGV
                     GGPRTMELRAARLDIASEGDIRSIVEDDPQVKAGLGRYRVSDVGSEKGQVSEAQAPSN
                     LYSDASIESIESCITKVRRSQSYPMISVLAREATFKSQPAYLLAYVWTSSTEDDAPLD
                     RVQAWVVSPSQCATLTYVSFKPR"
     gene            complement(2771..3163)
                     /locus_tag="VI541_00605"
     CDS             complement(2771..3163)
                     /locus_tag="VI541_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69440.1"
                     /translation="MRASAVGMKCPDCARLPRRATRAAPPRIYLAASATAFGLAGGLG
                     AITSLFGIGFLGFFFPLLTAFVIGSLVDRVAKRTGGAAMKALAALMTFTGLILGSLAV
                     GVPLFAFAGGRLINPLIAAGVVALLVGR"
BASE COUNT          645 a         1074 c         1035 g          588 t
ORIGIN      
        1 gaatccttga cgctggacag acgatcgcgt gtggtcatcg tgcatctgac caagctgact
       61 ccaactcatc tgatccagcg gatctctttt cttagcaggt tctcggtggc cacggcaacg
      121 gcgcgccggt cctacggctg agtgtgcgtg agataagtct cgctgggcgt taccgcctcg
      181 ttgaggttct ttcgcgtcag gagaacatcg tcagcgccct ggcaaccgat gaggtgttgg
      241 gacgatccgt gatggcccgg atcctcaaac agggcgtgcc tgaggagctg atctccacct
      301 tccgcaggtc cgcccttgca tgggctgcac tcactcatcc caacatcgcc acggtgtacg
      361 acgcgggcag gcacaggggc ctggagtacc tggtcagcga gttcgttccc agaggtaccc
      421 tcgccgacaa gttggccgcg ggccccctcg acccgatgag ggtctgcgag atcggcgacc
      481 aactctgtgc ggctctctct catgcgtctg cggccggcgt cggccacggc cacatcacag
      541 cggacaacgt cctgatcacc gagtcggggc aggttcggtt gaccgacttc ggcgtgcacg
      601 cggcgctagg tggcgtggtt ccgaacgctg ccgacgacat ccgggacgta gggcagatcc
      661 tgtttcgtgc cctgacaggt cgcgcccttg atgagtgccc cacgaactcc ccccgtgacc
      721 agaggcccga cgtacctcga ttcctcgatg aggcgatcgt tcgcgcgaca tcagaggagg
      781 gttatcagga tgccgccgca ttcgctgccg ccctcggagt cgcggcgagc ctcccggccc
      841 cggctcgcgc cgccccgagc gtaacggaac ctcctggaga acaggcatcc ttcctacgca
      901 gtgaagcgcg gctgttcctg acgatcgcat tgatagtggc ggtagccgct gcgatcgttc
      961 tcgctatccc taccatcacc cggaacatcg caccgaccgc gcagaccaga gctactccgg
     1021 tggtccttgg cgctgtgggc tcgggcatct atgaccctcc accgggcgac ggccacgaga
     1081 acgacgaact cgtctcattg gcattcgatg gggatcgacg gagcgcgtgg cgaactgacc
     1141 ggtacgggaa cgcctcattc gggagtctca agtccggcgt cggcatctgg gttgacctcg
     1201 gcaaaccgcg tcggatcacg tccgtgatca tccacaccgg acatcctgga tggctcggga
     1261 cgcttagata ctccgaagac tcggtcatct ggacggaccc aggtgccagc gaggaggtcg
     1321 aggacggtca cgctttctcg gtcaaccaga cacaccgata cgtgatgctg tggatcgcgc
     1381 gggtcgtccc ggtcgaaccc gcagacaaga atcccttcca ggcagacgta accgagtttc
     1441 gagccctcgg cggcggtgcg acatgagcga acagatctcg gaaaacgccg cggacctcgc
     1501 attggtgaaa gcattcgtga gcgaccacga catagacgcg ctcgagcaac tcctcgagaa
     1561 gcaccgcaac cggatattca ctcacgcgtt ccgggtcact cgaaaccgct cagacgcgct
     1621 cgatgtgacc caagaggtct tcatcaccgt ttacaagaag tcctccagct tccggggggc
     1681 ctcgagtttc gacacctggc tataccgatt gaccactaac gcctgctacg acctacttcg
     1741 caagcgcgca cgtcaacccg tggcctcagc cgagatccca gaaggttcgg gacgagtcga
     1801 cgatgagatc gcacgggtgg acgaccggct cgcggtcgaa gcggcgctgg ccagcctctc
     1861 gcccgagcat cgagccgcac tggtgatgcg ggagctggag gacatgacct acgaacagat
     1921 cgctgaggta ctcgaggtcc cagtggggac cgtgaagagc cggatagctc gtgggcgcgc
     1981 cgctctcgca gatctcgtga cggaacatca gagccaaccc gggcgtcaaa gggatgagtg
     2041 acatgcatga acatctagac gacgaggccc tcagcgggct cttggacacc gacggcatag
     2101 agaccatctc ctcatgcccc cgatgctcgg gccgctaccg agcgatgctg agcgcgtcca
     2161 cgatccttcg cgagatcgat ccggccccca tgacggcgga cgaggtgagg gagcttcgcc
     2221 ttgggatcct cgcggcaacc gcacggaggc cccctgctag atggatcgct gcagccgctg
     2281 gatccgttgc gatcctggtc gcggtagggc tctatatatc agggccgcgg ccgcgcaacg
     2341 acggtgttgg ggggcccaga acgatggagc ttcgggctgc ccggttggac atcgcatccg
     2401 agggtgatat ccgctcgata gtcgaggacg acccacaggt caaagcgggc cttggacggt
     2461 atcgagtttc ggatgtcgga tccgagaagg gccaggtctc cgaagcgcag gcgcctagca
     2521 acctctacag tgatgcgagc atcgagagca tcgagagctg catcaccaag gtgcggcgct
     2581 cccagtcgta cccgatgatc tcggtgcttg cccgtgaggc caccttcaag tcgcagcccg
     2641 cataccttct ggcatatgta tggacatcgt ccaccgagga cgatgcgccc ctcgaccgtg
     2701 tgcaggcctg ggtcgtctca ccctcccagt gcgcgactct gacctacgtc tcgttcaaac
     2761 cgcggtagct tcagcggcca accaggagcg ccaccacgcc cgcagcgatc agggggttga
     2821 tgagtcggcc gcctgcgaat gcgaagaggg gaacgccgac cgcaagagag cctagtatca
     2881 aacctgtgaa tgtcatcagg gccgcgagcg ccttcatcgc agccccaccc gtgcgcttcg
     2941 cgacccgatc tacgagcgac ccgatcacga atgccgtcag cagagggaag aagaacccca
     3001 gaaatccgat accgaagagc gaggtaatcg ccccgagtcc tcctgcgagc ccaaatgccg
     3061 tggccgacgc cgcgagatag atccttggag gagccgcgcg ggtggcacgg cgcggtaacc
     3121 tcgcgcagtc cgggcacttc atccctaccg cagacgccct catgcacttc gtgcagatgg
     3181 gtcggtcgca ggacgaacag cgcacgcgcg tctcacgaga agggtgcctg taacaggtgg
     3241 gctctttttc taccgacatc ggtgcctagg ctaccgctac cgctgcgagt atcattcgaa
     3301 tcatgaatcg tcgggttgtg gtgtcggtgg cggccgccct cc
//
LOCUS       DATDOZ010000032         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1332921, whole genome shotgun sequence.
ACCESSION   DATDOZ010000032 DATDOZ010000000
VERSION     DATDOZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332921"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VI541_00610"
     CDS             <1..634
                     /locus_tag="VI541_00610"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF69441.1"
                     /translation="AEAVHRDAHGMLASKIMEVDPNVVEQFLGRIEEAPIGEPRAASG
                     LRTILVTDIEGSTSMVQELGDDAAIRVIREHEGIIGACLRRANGRMVKGLGDGVLASF
                     DSIVRALECTLDIRERLHERNQTSDQPLRVRVGLSAGEPVSENDDLYGSAVTMASRIC
                     AVCRPGAILTSSTVRDLALGKGFSWDDQGQVTLKGFDDPVHVYSLTGRSG"
     gene            637..900
                     /locus_tag="VI541_00615"
     CDS             637..900
                     /locus_tag="VI541_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69442.1"
                     /translation="MTETAPSFSTAVERAYLKELRSREAASSFADASARSPLLTVHEE
                     ILAVAIYQADPNGGGLALKRLPNKQELEERLLEALRRSALTDQ"
     gene            complement(904..1368)
                     /locus_tag="VI541_00620"
     CDS             complement(904..1368)
                     /locus_tag="VI541_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69443.1"
                     /translation="MLARLAREIPSKVEVDRAGGFMRLTGEPAEADMQVAIDALSQIG
                     YEASRVTEEEDLPTEWFDAHSVIELSLTEADALADRWSGRYAEESADAATPQVRELLF
                     SVISNSFKAAHGGGPFPSTGRIRADLVTRAASLPEEIRARFIEWVGRATLGG"
     gene            complement(1440..1937)
                     /locus_tag="VI541_00625"
     CDS             complement(1440..1937)
                     /locus_tag="VI541_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF69444.1"
                     /translation="MSSILDHIYEQNAWANLAMLDLVESLTDEQLDAPTIDGTMGTIR
                     QMITHIANSQEGYVAGLSGVTREPWTEFPGFPALRDSLTRTNRKILEFANQVPDRTII
                     RTWNEQDYEIEPEVFLVQAINHATEHRIHIKVALTQLEVEHPFFDGWGYGGAKGLFKT
                     KESVG"
     gene            complement(2033..>2543)
                     /locus_tag="VI541_00630"
     CDS             complement(2033..>2543)
                     /locus_tag="VI541_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLF69445.1"
                     /translation="DRLGLGRDSEKIGAALAAELTAAIEDATSEVGTRVVEGAVEAVD
                     SLRAAGIPTALICDTGFTPARHVRRFLDQHGLKLDHYFFSELVGAPKPLPPMFQAALT
                     ATASPPERAIHIGDLKSTDIAGARAAGMATIRFIGVHDDAWAEEESRGEEADEVLGAW
                     QDLPSLLGL"
BASE COUNT          476 a          760 c          812 g          495 t
ORIGIN      
        1 ggcagaggct gtgcatcgcg atgcccacgg gatgctggca tccaagatca tggaagtcga
       61 tccgaatgtc gtcgagcaat tcctggggcg gatcgaggaa gcaccgatcg gcgagccgcg
      121 cgcggccagc gggttgcgaa ccatcctagt gacggacatc gagggctcca cctcgatggt
      181 gcaagaactt ggagacgacg cggctatccg tgtgatccgg gaacatgaag ggatcatcgg
      241 ggcttgtctg cgaagagcca atggtcggat ggtcaagggt ctcggcgatg gcgtgctcgc
      301 atccttcgac tcgatcgtgc gcgctctaga gtgcacgctc gacatccgag agcgcctgca
      361 cgagcgcaat cagacaagcg accagccgct tcgagtgcgg gtcggcttga gcgcaggtga
      421 gccagtcagc gagaacgatg acctctacgg gtctgccgta acgatggcat cgcggatctg
      481 cgccgtctgt cggcctggtg cgatcctcac ttccagcacg gttcgtgatc tggctcttgg
      541 gaaggggttc agctgggacg atcagggcca ggtcaccttg aagggtttcg acgatcctgt
      601 tcatgtctac tctctgaccg ggcgttcggg gtaggcgtga ccgagacagc gccgagcttt
      661 tcaacggcag ttgagcgtgc gtatctgaag gagttacgca gccgcgaggc cgcatcttcg
      721 ttcgcggacg cgagcgctcg ctccccgctg ctcacagtcc acgaggagat acttgctgtg
      781 gccatctacc aagctgatcc caacggcggg ggccttgcgt tgaagcggct tccgaacaag
      841 caagagctcg aagaacgtct gctcgaagca ctccgtcgct ctgctctcac cgatcagtga
      901 gacctagcct ccgagcgtag ctctcccgac ccactcgatg aacctcgcgc gtatctcttc
      961 gggtaatgat gcggcgcgag tgacgagatc cgcgcggatc cgaccggtcg aagggaaggg
     1021 gcccccaccg tgagcggctt tgaacgagtt ggagataaca gagaagagaa gttcccggac
     1081 ctgcggcgtc gccgcgtctg cggattcttc cgcgtatcgt cccgaccagc ggtcggcaag
     1141 ggcatccgcc tccgtcaggg atagttcgat gacactgtgt gcatcgaacc actccgtggg
     1201 aaggtcctcc tcttcagtta cccgagatgc ctcgtaaccg atctgcgaaa gagcgtcgat
     1261 agcgacctgc atatccgcct cagcaggttc gccggtgagc ctcatgaaac cccccgcgcg
     1321 atcgacctcc accttggatg ggatctcacg ggccagtcgc gccaacacgg gcgcgatgag
     1381 ggagcctcag ccgagccccc ggaccgcgct tcagccaagg ccactgaggc gaaggacgat
     1441 caaccgaccg actccttcgt cttgaacaat cccttcgcgc cgccgtagcc ccagccgtcg
     1501 aagaatgggt gttcgacctc gagctgagtg agagcaacct tgatgtggat gcgatgttcg
     1561 gttgcgtggt tgatggcttg aaccaagaaa acctcgggct cgatctcgta atcctgttca
     1621 ttccatgtgc ggatgatggt gcggtcgggc acctggttgg cgaactcgag gatcttgcgg
     1681 ttcgttcgag tcagcgagtc gcgcagggcc ggaaaaccgg ggaactcggt ccacggctcg
     1741 cgggttacgc ccgaaagacc tgcgacgtag ccctcttgag agtttgcgat gtgagtgatc
     1801 atctgacgga tggtccccat agtgccgtcg atcgtcgggg catccaactg ctcgtcggtg
     1861 agactctcga cgaggtcgag catcgctagg ttggcccagg cattctgctc gtagatgtga
     1921 tcgaggatag aactcatacc ggctccttct atagggcacc cgattctatg tccttgcgaa
     1981 cttgggcgaa aaagcgtcgg tagccgatgg tttttcgccc aagttcgggg ggttagaggc
     2041 cgaggagcga ggggaggtct tgccaggccc ccagaacctc gtcggcctct tcgccacgag
     2101 actcctcttc ggcccatgca tcgtcgtgaa caccgatgaa cctgatcgtc gccatccctg
     2161 cggccctggc gcctgcgatg tcggtgcttt tgaggtcgcc tatgtggatc gctctctccg
     2221 gcggtgatgc cgtggccgtc agcgcggctt ggaacatcgg cgggagcggc ttgggggccc
     2281 cgaccaactc cgagaagaag tagtggtcga gtttcaatcc atgttggtcc aggaaacggc
     2341 ggacgtgacg ggcgggtgtg aaaccggtgt cacagatgag ggccgtgggg atccccgcag
     2401 cgcgaagcga gtcgaccgct tcgaccgcgc cctcgacgac tctggtcccc acctcggagg
     2461 tcgcatcctc gatagccgcc gtcaactcgg cggcaagggc agctccgatc ttttcggaat
     2521 ctcggcccaa ccccagcctg tcg
//
LOCUS       DATDOZ010000033         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3166831, whole genome shotgun sequence.
ACCESSION   DATDOZ010000033 DATDOZ010000000
VERSION     DATDOZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166831"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2517
                     /locus_tag="VI541_00635"
     CDS             <1..2517
                     /locus_tag="VI541_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLF69446.1"
                     /translation="WMALKEGGKAFYTTPLKALSNQKFGDLVALHGAGEVGLLTGDNS
                     INPEAPIVVMTTEVLRNMIYERSALLDDLRYVILDEVHYLQDRYRGAVWEEVIIHLPV
                     DVKIVSLSATVSNAEEFADWIQTLRGPTTAIIEEARPVKLEQHYLVDSEIYPMFISTG
                     DEQIPNPEIRRLESRSNRPRSRRGRHPSHQYTRKLARRTDVVDLLSAGHMLPAIYFIF
                     SRKGCNGAVSQCVREGIDLVDQDERKRIGDYADARCSYLAQEDLAVLGYDEWLRALKS
                     GIAAHHAGLIPAFKETVEELFQAGLVKVVFATETLSLGINMPAKSVVIESLFKFTGER
                     HEVLTPGEYTQLTGRAGRRGIDVLGHSVVPQQRDATFQQIAGLASTRTYPLISSFQPS
                     YNMATNLIRNYSRHEADHLLNSSFAQYRADKDVVVLERLIERNEGYLASYREKMHCDR
                     GEFSAYWELREQVTALEKSISRWAMTAGKEQTKSVLDLARPGQVLMVTTGKMRGPVAV
                     VGHERSRRGDPRVLALTRNGRLVRLSASDFPRAPRVVGSLQEMKGRSGLNSFNSAKSI
                     DHDTKRKLVHALEGLVLPDDAYDPTPLPEEEQGSGLDITKQKLLQHPCHSCEDRDKHA
                     QWAERAARLEKENDGLIKRVRRRTETLSRRFERVLGILEEYAYVDGFNLTEKGWLLAR
                     IYNENDLLVAEALAKGWLAGLKPAELAAVASTFVFESRGPIEVVGTLPTQDSKKVFGK
                     VVRLTERIKRAESRAGLEMTRGPESGFADVVYRWCQGSALEDVIDDDSTPGDFIRGCK
                     QTIDLLKQMSQAAGSSELRQALDDAVDGVNRGVVAYTGVL"
BASE COUNT          584 a          704 c          824 g          526 t
ORIGIN      
        1 tggatggccc tcaaggaggg cgggaaggcc ttctacacga ctcctcttaa agcgctgtcc
       61 aatcagaagt tcggtgatct ggtggcgctt cacggtgctg gcgaagtcgg actcttgacc
      121 ggcgacaact cgatcaatcc cgaggccccc atcgtcgtga tgacgaccga agtgcttcga
      181 aacatgatct acgagcggtc agctctactc gacgaccttc gctacgtgat cttggatgag
      241 gtccactatc ttcaagacag gtaccgcggt gctgtttggg aagaggtgat catccatctt
      301 cccgtcgacg tcaagatagt ctcactatca gccacggtct ccaacgctga ggagttcgcc
      361 gactggatac aaactctgcg ggggccgacc accgcgatca tcgaagaggc gcgtccggtg
      421 aagcttgagc agcactacct tgttgactca gagatctacc ccatgttcat ttcgacgggc
      481 gacgagcaga taccgaaccc agagatccga aggctcgagt cacgttctaa tcggccgcgg
      541 tcccggcgcg gaaggcatcc cagtcatcaa tacacgcgca agctcgctcg cagaaccgat
      601 gttgtggacc tgttgtcagc tggtcacatg ctccctgcca tctacttcat cttctcgagg
      661 aagggatgca atggagcggt ctcccagtgt gttcgagagg ggatagatct cgtagatcag
      721 gatgaaagga agcgcatcgg ggactatgcg gacgcgagat gcagctacct cgcccaagaa
      781 gacctcgcgg tgcttggcta cgacgaatgg ctcagggcgc tgaagtcagg gatcgcggca
      841 catcatgctg gccttatccc agcgttcaaa gagaccgtgg aagagctgtt ccaggcaggt
      901 ctcgtcaagg tggtgttcgc gaccgagacg ctgtccctcg ggatcaacat gcctgcgaag
      961 tccgtggtca tcgagagctt gttcaagttc accggcgagc ggcacgaggt gctcactcct
     1021 ggggagtaca cgcagttgac aggaagggcg ggccgaaggg gaatagatgt acttggccac
     1081 tccgtcgttc cacaacagcg cgacgctacg tttcagcaga tcgcaggtct ggcatctacg
     1141 aggacgtatc cgctcatctc gtctttccaa ccgtcctaca acatggcaac gaacctgatc
     1201 cgtaactact cccgtcacga agcagaccac ctgcttaact cttcattcgc ccagtaccgg
     1261 gccgacaagg acgtggtcgt gctggaaagg ctgatcgaga ggaacgaagg ttacctggcg
     1321 tcgtatcggg agaagatgca ctgcgaccgc ggagagttct ctgcctattg ggagcttcgc
     1381 gagcaggtaa cagcgttgga gaaatctatc tcacggtggg ccatgaccgc aggcaaggaa
     1441 cagacgaagt cggtcttaga cctagccaga ccgggtcagg tgctgatggt gacaacagga
     1501 aagatgaggg gccccgtcgc tgtcgttggt catgagcgat cgcgccgcgg agaccctcgc
     1561 gttctggctc ttacgcgtaa cgggcgcctg gtgagactta gcgcgtcgga tttcccacgg
     1621 gctcctcggg tggttgggtc tctgcaggag atgaagggaa ggagcggact caactcgttc
     1681 aacagtgcta agtcgatcga ccacgacacc aagcgcaagc tagtacatgc gcttgagggt
     1741 ctggttcttc ccgatgacgc gtatgacccg acgccccttc ccgaggaaga acaaggttcg
     1801 ggtctggaca tcacgaagca gaagctgctt caacatccgt gccattcgtg cgaggacaga
     1861 gacaagcacg cgcaatgggc tgagcgggcg gcgaggttag agaaggagaa cgacggcctc
     1921 atcaaacgcg tgagacgacg caccgaaaca ctgtcgcgtc ggtttgaacg ggttctcggg
     1981 atccttgagg agtacgccta cgtggatggc ttcaatctga ccgagaaggg atggctgctc
     2041 gctaggatct acaacgagaa cgacttgctg gtagcagagg ctctggcaaa gggatggctg
     2101 gccggcctta agccggcgga gctggctgcc gttgcttcga cattcgtctt cgagtctcgg
     2161 gggcccatcg aagtcgtggg gacgctgccg acgcaagact cgaagaaggt gttcggcaag
     2221 gtggttcgct taacagaacg gatcaagagg gcagaatcac gtgcaggatt ggagatgaca
     2281 agaggaccag agtctgggtt cgctgacgtt gtgtacagat ggtgtcaagg cagtgctctc
     2341 gaggacgtga tcgatgacga ctccacgccg ggcgacttca tccgaggatg taagcaaacg
     2401 atcgatctgc ttaagcagat gagtcaagcg gctggatcga gtgagctgag gcaggctctt
     2461 gacgatgcag tcgatggagt taatcgaggc gtggtcgcat acaccggggt tctttgatgg
     2521 tcgtctcttc ctgtgattag accggcctgc gaggttcctt cggaacgact ggatcaatac
     2581 gcggccgatt ggtgggctct tgcgatgatg cgccagcgac ggggagagcc attcgccc
//
LOCUS       DATDOZ010000034         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1597305, whole genome shotgun sequence.
ACCESSION   DATDOZ010000034 DATDOZ010000000
VERSION     DATDOZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597305"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..367
                     /locus_tag="VI541_00640"
     CDS             41..367
                     /locus_tag="VI541_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69447.1"
                     /translation="MKRRIAPLALAAMVSTVVVSGAFADPPGGEPTLHTQGAGNAGDG
                     SIYVTSGGVYLESNTEAGLQSTAGFHADDPNTSEDEYHAWDADESLDPSLIPALRRKI
                     EKVLER"
     gene            complement(508..582)
                     /locus_tag="VI541_00645"
     tRNA            complement(508..582)
                     /locus_tag="VI541_00645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(545..547),aa:Pro,seq:tgg)
     gene            complement(694..764)
                     /locus_tag="VI541_00650"
     tRNA            complement(694..764)
                     /locus_tag="VI541_00650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Gly,seq:tcc)
     gene            801..1262
                     /locus_tag="VI541_00655"
     CDS             801..1262
                     /locus_tag="VI541_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69448.1"
                     /translation="MTIGTDYLALLYLCQNIFPSSASHALGHVKQLVPQVIELEDEYV
                     ALTAVDAGMFPQESDYVSHAHIEYAHLPISCVIDVTLTILRVVFLLIRRPTKSAVVVQ
                     LPSFDAPPGKVRRRLTKTAAATPAKNDRAAGGLLYWKHPLLIHASNCREGV"
     gene            1297..2667
                     /locus_tag="VI541_00660"
     CDS             1297..2667
                     /locus_tag="VI541_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLF69449.1"
                     /translation="MLDLQKIRDHVEGNREKYIESLKEACRIPSVSTEGASFGAMADW
                     VQARLEALGASVARLKVGESPEALLGVIPGGGEGTLMIYDHYDVQPVDPIELWDSDPF
                     EPVERDGRIYARGAADNKGDLVARLCALEAYRELGDELPYTIKFFIEGEEENGSTHFE
                     KICNEYAAELASDNCVWEGGWFDFDDRPHMYYGCKGLLYVELSVQRLSGDQHSSIAVY
                     APSAAWDLLRAIACLKDPDGNIVIDGFHDDIVPAGPRERAMLESLPFNEDAIKANLKI
                     DAFNAGLTGEELKHDLLFKPTANIAGLISGYTVPGASKTVLPAEAMAKIDFRLVPDQD
                     PEDLATKLRAHLDKNGFEHVDVRVLNGENPSRSTFDTALARAVEGTAARWFSQPTAVM
                     PFMWATGPMHPLAQGLGIPICSPPGVGRPDSNLHAPNENARIADYLDIVGFTVAYLDA
                     YGQTVD"
     gene            2733..>2927
                     /locus_tag="VI541_00665"
     CDS             2733..>2927
                     /locus_tag="VI541_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69450.1"
                     /translation="MRVQAGLLGAAIIIVLGAALFTIVNPVRGPITLPDDSDTNTQVS
                     PTPSISPTPQASPSPSVEPTG"
BASE COUNT          619 a          899 c          844 g          565 t
ORIGIN      
        1 agcgtgcggg cgatccgcac cgaggtgaag ggagcaagac atgaagcgtc gcatcgcgcc
       61 tctcgccctg gctgccatgg tgagcacagt tgttgtaagc ggagcattcg cagatcctcc
      121 aggcggggaa ccaaccctcc acacgcaggg cgctggcaat gccggcgacg gcagcatcta
      181 cgtcacctcc ggcggcgtct accttgagtc gaacaccgaa gctggtcttc agagcacggc
      241 tggctttcat gccgatgacc caaacacctc ggaggacgag tatcacgcgt gggatgctga
      301 cgagtcgctt gaccccagtc taatccccgc tctgcgccgc aagatcgaaa aagtcctaga
      361 gagataacgg aaggaaggcg tatctaactt gtctagttgc taagtaagaa acggccccca
      421 ctcgggggcc gtttctcttt ttgcccgtga cctcgacggt caaccaaccc cgtaagctgc
      481 ccacgacccg atggtcgata aacgcagtcg ggacggggag attcgaactc ccggcctcgg
      541 cgtcccaaac gccgcgcgct aaccaagctg cgctacgtcc cgatactgct cagtctaacg
      601 gttgacgtct atgggcttca gccgcgcttg ggttcctatt cgttagcgga gccgcgctgc
      661 aaagcgatgc gggcccccaa gatcgcaccc gagagcggcg agcggggatc gaacccgcgt
      721 aatcagcttg gaaggctgag gctctaccat tgagctaccg ccgcacgaag aaaattccgt
      781 gacttcgatg ttctcctacg atgacaattg gcacagacta cctggcactt ctctatctct
      841 gccagaacat cttcccatct tcggcttcgc acgccctggg tcacgtcaaa cagcttgttc
      901 cccaggtgat cgaactcgag gacgagtatg tcgctctcac cgcagtcgac gcagggatgt
      961 tccctcagga atcggactat gtaagccatg cgcacattga gtatgcgcac ttgcctatct
     1021 cgtgcgttat cgatgtaacg ctgacgattc ttcgcgtagt gttccttctt atacgccgcc
     1081 cgacaaagtc ggcagtagtt gtccaactgc cctctttcga tgcgccgcca ggcaaagtcc
     1141 gcaggaggct tacgaagacc gcagcggcca cacctgcgaa gaatgaccga gccgcgggtg
     1201 gcctcctcta ttggaagcat ccgttgttga tccacgcatc caattgtagg gagggggtct
     1261 gacaggtagc ctggcgaatc acgatgagga gggacgatgc ttgacctaca gaagatacgt
     1321 gaccacgttg aaggcaaccg cgagaaatac atcgagtcgc tgaaagaggc ctgccgcatc
     1381 ccaagcgttt cgacggaagg ggcctcgttc ggtgcgatgg ccgactgggt gcaggcgcgc
     1441 ctcgaagcac tcggtgcctc ggtcgcaagg ctcaaggtcg gcgagtcacc ggaagcattg
     1501 ctcggtgtta tccctggcgg tggcgaaggc acgctcatga tctacgacca ctacgacgtc
     1561 cagccggtcg acccgatcga gctatgggat tcggatccat tcgagccggt ggagcgcgac
     1621 gggcggatct acgcgcgggg ggccgccgac aataagggag acctcgtagc gcgactctgc
     1681 gcgctcgagg cctaccgcga actcggggac gaacttcctt acacgatcaa gttcttcatc
     1741 gagggcgaag aggagaacgg gagcactcac ttcgagaaga tctgcaatga gtacgccgcc
     1801 gaattggcgt ccgacaactg cgtctgggag ggagggtggt tcgacttcga tgaccgtcct
     1861 catatgtatt acggatgcaa gggtctgctc tatgtcgaac tcagcgtgca gcgtctgagc
     1921 ggggatcaac actcgtcgat cgcggtgtat gcaccttcgg ccgcctggga cctgcttcgc
     1981 gctatcgctt gtctgaaaga tcccgacggc aacatcgtca tcgacggatt ccacgacgac
     2041 atcgttcccg ccggtccccg ggaacgagcg atgctcgaga gtctcccttt caatgaggac
     2101 gcgatcaaag ccaacctgaa gatcgatgcg ttcaatgcag ggttgaccgg agaagaactg
     2161 aagcatgacc tgctgttcaa gccgacagcg aatatcgcgg gtctcatcag cggttacacg
     2221 gtgccggggg cctcgaagac cgtcctcccc gccgaggcca tggcgaagat cgatttcaga
     2281 ttggtcccgg accaggaccc cgaggacctc gccaccaaac tgcgcgctca tctcgacaag
     2341 aacggattcg agcatgttga cgtgagggtc ttgaacggcg aaaacccatc gcgctccaca
     2401 ttcgacaccg cgctcgctcg agccgttgaa ggaactgcag cgagatggtt cagtcagcca
     2461 actgcggtca tgccattcat gtgggcgacg gggcctatgc accctctcgc ccagggactc
     2521 gggatcccga tctgctcacc tcccggtgta ggtcgacctg attccaacct gcacgcgcca
     2581 aatgagaatg ctcgtatcgc tgactatctc gacatcgttg gattcaccgt cgcgtatctc
     2641 gatgcttatg gccaaacggt tgactaatgc ccctaagggc cagggcccac tgctagactg
     2701 acctccaacg caacggcagc aggggtgaat ctgtgagagt tcaagcgggc ctgctggggg
     2761 ccgcgatcat catcgttctc ggcgcggctc tgttcaccat cgtgaacccc gtgcggggac
     2821 ccatcaccct gcctgatgat tcggacacca acacgcaggt ttctcccacc ccttccatct
     2881 caccaacacc gcaagcttcc ccctcgccct cggtcgagcc gaccggt
//
LOCUS       DATDOZ010000035         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1333227, whole genome shotgun sequence.
ACCESSION   DATDOZ010000035 DATDOZ010000000
VERSION     DATDOZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333227"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="thiI"
                     /locus_tag="VI541_00670"
     CDS             complement(<1..966)
                     /gene="thiI"
                     /locus_tag="VI541_00670"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HLF69451.1"
                     /translation="MSTRHDLVVAHYHEVGLKGRNRGYFERLLAQNIRDSLAGTGYSK
                     VSLISGRVLIGLGPDHDLGEVRSRLSKVFGLSGFSPAISVEPSMDALIATALEFASQS
                     QFSTFAVRARKGNSSFPDSSSTVNEVVGQAINDASSARVDLSNPEWTCHIELVENRAF
                     MYHERCEAPGGLPVGSAGRALALLSGGIDSPVAAWLIAKRGAGIDFIHFHGQPFADPS
                     SARQATRLARALKPWLLRSRLWLIEFGPIQSEIVTAAPQELRIVLYRRFMMRIAERLA
                     MREGAEALVTGESLGQVASQTLPNMAAIDKVVETLPVLRPLVGSDK"
     gene            complement(963..1259)
                     /locus_tag="VI541_00675"
     CDS             complement(963..1259)
                     /locus_tag="VI541_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLF69452.1"
                     /translation="MINAIVLLKVDVHSIPETAQRIAALERVSEVYSVAGEWDLVVVI
                     RVKEHDELASVITGQLDRIEGITRSTTLIAFQVFSKHDLEAMFSVGFEGAAPSP"
     gene            complement(1334..2287)
                     /locus_tag="VI541_00680"
     CDS             complement(1334..2287)
                     /locus_tag="VI541_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF69453.1"
                     /translation="MEYEPTRLEVATEAPHEDLVRQYLREIGQYPLLNDVGEVELAKA
                     YEAGQAATVTLGDPKKKITPKRKIELKRIEQQGQAAKRRFIQSNLRLVVSIAKKYSAA
                     GLPLLDLIQEGNLGLMRAVEKFDYRRGFKFSTYATWWIRQAITRAIADKARTIRIPVH
                     MVETLYRVRKVQAELLENLGREATIDEIAAAAEMTPDKVREAFRVLPEPVSIHEPVGD
                     EDAELGDFIQDDDAQGPFEAAALILRQEDLWSMLGTLSDREKKVLALRFGLVTGEPRT
                     LEEVGREFALTRERIRQIEAKALSKLRHPSSPGKLRDMVTL"
BASE COUNT          470 a          771 c          797 g          512 t
ORIGIN      
        1 cttgtcgctc cccacgagcg gtcgcaggac cggcaaggtc tcgacgacct tgtcgatcgc
       61 cgccatgttc ggaagggtct gagacgcgac ctgcccgaga ctctcaccgg tcacgagggc
      121 ctcggccccc tcacgcatcg ccagccgttc agcgatgcgc atcatgaacc gtcgatatag
      181 aacgatccgc aactcctggg gtgccgccgt gacgatctcg gactggatcg ggccgaactc
      241 gatgagccag agccgcgatc gcaggagcca tggtttcaat gcccgcgcaa gtcgagtcgc
      301 ctgacgtgcg ctggacggat ccgcgaatgg ttgtccgtgg aagtggatga agtcgatgcc
      361 cgctccgcgc ttggcgatca gccatgcggc gacgggggag tcgatgcccc cggagagaag
      421 cgccaaggct cggcccgccg agccgaccgg caggcccccg ggggcctcgc agcgttcgtg
      481 gtacatgaat gctcggttct cgaccagctc tatgtggcag gtccactcgg ggttggacag
      541 gtccacgcga gcactgctgg catcgttgat cgcttgtccc acgacctcat tcacggtcga
      601 agagctgtcg gggaaagagg agttgccctt gcgggctcga acagcgaacg tcgagaactg
      661 gctctgcgac gcgaactcca atgccgtcgc gatgagtgca tccatcgacg gttccaccga
      721 gatggcaggg gagaaccctg agagcccgaa gaccttagag agccgagagc gcacctcgcc
      781 gaggtcgtga tctggtccaa ggccgatgag gacacgtccc gagatcaagg agaccttgct
      841 gtagccggtg ccggccaaac tgtcgcggat gttctgagca agcagacgct cgaagtaacc
      901 tcgattgcga cccttcagcc cgacctcgtg atagtgggct acgacgaggt cgtgccgtgt
      961 actcatggac tcggcgcggc tccctcgaac ccgacactga acatggcttc gaggtcatgt
     1021 ttgctgaaca cctgaaaggc gatgagtgtg gtgcttcggg tgatgccctc gatcctgtcg
     1081 agctgaccgg tgatgaccga tgcaagctcg tcatgctcct tcacgcggat cacgacaacc
     1141 agatcccact cgccagccac cgagtagacc tcggaaacgc gctcaagggc ggcgatccgc
     1201 tgtgccgtct cggggatgga gtggacgtcg accttcagca agacgatggc gttgatcatg
     1261 tgttcatgct acctcgcccc gttctcgggc ggattaccac cggtagccga cgcttattcg
     1321 cccaagttcg ggactagaga gtgaccatgt cgcggagctt gccagggctc gaggggtggc
     1381 gcagcttcga aagcgccttc gcctcgatct gacggatccg ctcacgcgtg agtgcgaact
     1441 cgcggccgac ctcttcgagg gtacgaggct cgcccgtcac gaggccgaac cgcagcgcga
     1501 gtaccttctt ctcgcgatcc gagagcgtgc cgagcatgct ccataggtct tcctgtcgaa
     1561 ggatgagagc ggcggcctcg aatgggccct gagcgtcgtc gtcctggatg aagtcgccta
     1621 gttcagcgtc ctcgtcgccg accggctcgt ggatcgagac aggctcggga agcacacgga
     1681 aggcttcgcg aaccttgtct ggagtcatct cggcggcggc ggcgatctca tcgattgtgg
     1741 cttcgcgccc gaggttctcg agaagttcgg cctgcacctt gcggacccga tagagggtct
     1801 cgaccatgtg tactgggatc cggatcgtac gggccttgtc ggcgatggcg cgggtgatgg
     1861 cctggcggat ccaccaggtg gcataggtcg agaacttgaa gccacggcgg tagtcgaact
     1921 tctcgacggc tcgcatcagg cccaggttgc cctcctggat gaggtcgaga agaggaaggc
     1981 ccgcggccga gtacttcttg gcgatcgaga ctacgaggcg caggttgctc tggatgaatc
     2041 gacgctttgc cgcctgaccc tgctgctcga tgcgcttgag ttcgatcttg cgcttgggag
     2101 tgatcttctt cttgggatcg cccaaagtga ccgttgcggc ctgccctgcc tcatacgcct
     2161 tggcgagctc tacttcacca acgtcgttca ggagtgggta ctgcccgatc tcgcgaaggt
     2221 actgacggac aaggtcctcg tggggggcct ccgtcgctac ctcgagccgg gtgggctcgt
     2281 actccatata atcctgctct ctcttcactg tcgatccttc cttagcaact gcatcagtcg
     2341 tgttgtatgc gggggttcgt tcaggattgt gctgacccga ccgcttgttc cgatttcgat
     2401 tgacctcctc acctggcaag gtcaatgaac cctgtaggag cgggaagaag attgtaggct
     2461 catctgacct acttgacaag accttgcccc gcaacctcac gaaatcttca tacatgtcaa
     2521 gtaacgcttg tgaaccgttt cacattacgg
//
LOCUS       DATDOZ010000036         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2900850, whole genome shotgun sequence.
ACCESSION   DATDOZ010000036 DATDOZ010000000
VERSION     DATDOZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2900850"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VI541_00685"
     CDS             complement(<1..721)
                     /locus_tag="VI541_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HLF69454.1"
                     /translation="MIVVAILLAVVGLILAIRRWPDPAQDSLFNRIGWGGVAIAVILV
                     LTSTVTIVPPGEVAVPVLFGSAQAPLEEGFHLRNPFASVRALSVRTQESTYTDTVGEG
                     EATDRADAITAVSLENASVRVEITILWHLVGAEASNVYRSLGTGYREVIVRPVSRSAV
                     RDCVSGYRFELARTTQRPEVERCIEDSLVEEFLPRGLAVDGVQLRDIAATPELQASIE
                     AKLQAEQDAQRAQFRQDQARID"
     gene            1060..>2066
                     /locus_tag="VI541_00690"
     CDS             1060..>2066
                     /locus_tag="VI541_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69455.1"
                     /translation="MRRRTLASLALATTTVFALASVGVATADPSPGQHPGGTNHLFGT
                     GANGNIELVGVERVTTTPGLVADVAVSPDGNWAFLANWGEPDCAGPETGGQNTPDAGA
                     WVVDIHDLANPETVGFIPSHQDSRPGEGMQVINLTTKNFSGQMLVMNNEQCGPQGKGG
                     VSLFDVTDPTQPKKLSEHFGDRSGLGNDSNDIHSAFAWDAGDNAYLVMTDNLEFPDVD
                     ILDITNPKRPRLIAEYDLNDFAVDQPEIGRIESFLHDMVVKEIDGHFIMLLSYWDGGY
                     VQLNVDDPTNAVFIGDTDFTNPDPELLESTGIALTPEGNGHQAEFNSDNRFFIGTDED
                     FG"
BASE COUNT          422 a          632 c          650 g          362 t
ORIGIN      
        1 catcgatccg ggcttgatct tggcggaact gggcgcgttg agcgtcctgt tcggcctgca
       61 gcttcgcttc gatggacgcc tggagctcgg gggtggccgc gatgtcgcgc aactggaccc
      121 catcgaccgc cagaccgcgg ggcaagaact cttcgacgag tgagtcctcg atgcagcgct
      181 cgacttcagg ccgctgcgtg gtccgggcca gctcgaatcg gtaccccgag acgcaatccc
      241 tcaccgccga tcgtgatacc ggtcgcacga tcacctcccg atacccggtg cccaacgagc
      301 ggtagacgtt cgatgcctcc gctccgacca ggtgccagag aatggtgatc tccaccctca
      361 ccgacgcgtt ctccaaggag acagccgtga tcgcatccgc tcgatctgtt gcttctccct
      421 cgccgaccgt gtcggtgtag gtcgactcct gggttcgaac ggagagcgca cgcaccgagg
      481 cgaacggatt ccgcaggtga aagccctctt cgagtggtgc ctgagccgaa ccgaagagga
      541 ccggcacggc cacttccccg ggagggacga ttgtcaccgt cgaggtcagc acgaggatca
      601 cggcgatggc gacgccgccc caaccgatcc tgttgaagag gctgtcctga gccggatcag
      661 gccatctcct gatcgcgagt atcaggccta caacggccag gaggatggca acaacgatca
      721 tcagaggttc cttgttctcg tcggtgatga cagggtgtgg gcgcgccggc tatcgaacgt
      781 tgttgtcctc gtatgggccg acggaatatc tcgcccattc aacgtcgcag acggtagtgg
      841 agcactactc gaagccgccg atcagcgagc gggtcggcgg cgagagacag tacctcatgc
      901 ctcgccgtcg aatcatggtt catccgagga cggaacgcag agagattggc attctcgggc
      961 ggacctgaca gtcgcacagt cacgcgggct tgcaggttta gaaacggaag cggttgggaa
     1021 ctgaataggg atcgtcagac ggagacacga ggaggtctca tgcgtagacg tactttggca
     1081 tccctagctc tcgccacgac caccgtattc gctctcgctt cggtcggagt cgcgacggct
     1141 gatcccagcc cgggacagca cccgggcggg acgaatcacc tttttgggac cggtgcgaac
     1201 ggaaacatcg agctggtggg ggtagaacgc gtcaccacca caccgggtct cgtcgcggac
     1261 gtcgcggtga gccccgacgg taactgggcc ttcctagcca attggggtga gccggactgc
     1321 gccggaccgg agacgggggg tcagaacacg ccggacgcgg gcgcatgggt agttgacata
     1381 cacgatctcg cgaatccaga aaccgtaggg ttcattccat cccaccagga ctctcgtccc
     1441 ggcgagggca tgcaggtcat caacttgacc acgaagaact tctcaggaca gatgctcgtg
     1501 atgaacaacg agcagtgcgg gccgcagggc aagggtgggg tgtccctgtt cgatgtcacc
     1561 gacccgacgc agcccaagaa gctgtctgag catttcgggg atcgcagcgg cctcggcaac
     1621 gactcgaacg acatccacag cgcatttgcc tgggacgcgg gcgacaacgc gtacctggtg
     1681 atgacggata acttggaatt tccggatgtc gacatcctcg acatcaccaa cccgaagagg
     1741 ccgcggctga tcgccgagta tgacctcaac gacttcgccg tggaccaacc ggagataggg
     1801 cgcatcgagt ccttcctgca cgacatggtg gtgaaggaga tcgacggtca cttcatcatg
     1861 ctgctgtcgt actgggacgg cggatacgta cagctcaacg tcgacgaccc gacgaacgcg
     1921 gtcttcatcg gggatacgga cttcacgaac cccgatccgg agttgctcga gtcgacgggg
     1981 atcgccctga ccccggaggg aaacgggcac caagccgagt tcaactccga caatcgcttc
     2041 ttcatcggca ccgacgagga cttcgg
//
LOCUS       DATDOZ010000037         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2901562, whole genome shotgun sequence.
ACCESSION   DATDOZ010000037 DATDOZ010000000
VERSION     DATDOZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901562"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1148)
                     /locus_tag="VI541_00695"
     CDS             complement(6..1148)
                     /locus_tag="VI541_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HLF69456.1"
                     /translation="MGGLLVVVLLSGCATSSASITSAPSPSASPRTTQTSEASPVASQ
                     VRAVSPALRAVIPSGISAELVSYVRATPGVAAAARISIDNISIRTDQGTSSISVAGVD
                     PTEFRPLAPDITAQAPFVWRGLARSEILLAHEEHERVGAEPGSVVFAQLRDKTLALRL
                     GGVAANGIPNIAGGLISLSRASLLGLGEPTILLIGIEDPSHFAEVRSALRKLIMGVEF
                     QDVRPLGRRAFLTGTAAARALGSFSFTANSDGSIDQDPRWVKRNIVNAKVPILGTVTC
                     HRAMIRQLSSALAEVERSGLAAVIDRKQYGGCYVPRFIGHDEAKGLSMHAWGLAVDLN
                     VSSNLQGSRPTIDPRVVEIFQRWGFRWGGDWSVPDGMHFELGALLR"
     gene            1320..1742
                     /locus_tag="VI541_00700"
     CDS             1320..1742
                     /locus_tag="VI541_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HLF69457.1"
                     /translation="MSERPDAAQLAEAIKGLSDEDLKVELEKWGIDTVLKDIFAGMQD
                     AFRPDKAGSNAALIQYDIDVDGDIRSWTVDIANGACKTSEGAAENPRLTLQLGLINFV
                     RLIFRQADGTQLFMSGKLKLKGDMMFAMQMQNYFESPA"
     gene            1752..2615
                     /locus_tag="VI541_00705"
     CDS             1752..2615
                     /locus_tag="VI541_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007258381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLF69458.1"
                     /translation="MESCIITAALSGVAADRSQCPAIPYTPDEYAAEAKRARDAGAAV
                     VHLHARHADGSPSYRVEEYRAITEAIKASAPDLIINYSTGAVGIPKEERIAHIVDLRP
                     EIGALNMGSMTYAKYSSKRKQMIFDFVFENRFETIVYFLEKMNEVGVKPECECFDTGH
                     IHNAIPLLDMGLLTAPVQFSLILGVLGGAPADARTLAHMASLVPQPATWEVIGISRDQ
                     WMLVTAAVALGGNVRVGLEDNFYKPSGEMASSNGDLVAAAAQIVQLSGRKVAEPDEAR
                     GLLDLKIPQGL"
BASE COUNT          523 a          805 c          845 g          478 t
ORIGIN      
        1 cggcgctagc gcagtagggc ccccagctcg aagtgcatgc cgtcggggac cgaccagtct
       61 ccgccccaac ggaagcccca ccgctggaag atctcaacga cgcgcggatc gatcgtcggg
      121 cggctcccct ggagattcga agatacgttg aggtcgaccg cgaggcccca tgcatgcatc
      181 gataggcctt tggcttcgtc gtgccctatg aagcgcggga cgtaacagcc cccgtactgt
      241 ttgcggtcga tcacggcggc gagccccgag cgctccactt cggcgagggc cgatgagagc
      301 tggcggatca tcgctcgatg acaggtaacg gttcccagga tgggaacctt ggcgttgacg
      361 atgtttcgtt tcacccagcg cggatcctga tcgatcgacc cgtctgagtt ggcagtgaag
      421 ctgaacgaac ccagagcgcg cgcagcagcg gtacctgtca ggaacgctcg cctgccgagt
      481 ggtcggacat cttggaattc aactcccatg atgagtttgc gcagtgcaga gcgcacctcc
      541 gcaaagtggg acgggtcctc gatgccgatg agcaggatcg ttggctcgcc gaggcccagc
      601 agactggcac gtgagagcga gatgaggccc ccggctatgt tcgggatccc gttggcggcg
      661 acaccaccga gccgcagagc gagggtcttg tcgcggagct gcgcgaagac caccgacccg
      721 ggctctgctc cgacgcgttc atgttcctca tgggctagga ggatctcgga gcgagcgaga
      781 cccctccaaa cgaagggggc ctgcgcagtg atatcggggg ccagtggccg gaactccgtg
      841 gggtcgacgc cggcgaccga gatagatgag gtcccctggt cagtccggat cgagatgttg
      901 tcgatcgata tccgcgcggc tgctgctaca cctggtgttg cccgcacata tgagacgagt
      961 tccgctgaga tgccgctcgg tatgactgct ctcagggcag gtgagacggc cctcacctgt
     1021 gatgccaccg ggctggcctc tgaggtttgg gtggtgcgag gagatgccga tggactcggt
     1081 gcgctcgtga tggatgcact cgacgtagcg cagccgctca gcagcacgac gaccaagagg
     1141 ccccccatcg cgcggacccg atccgttctc gggcgagata ccaccgggta ccggtggtta
     1201 ttcgcccgag aacggattga gagcatctgg aaagggtacc ggacctcggg agcaccgggg
     1261 caactcgcac ccgaaccgct gcaacggtta gggttgcaac aactagagga ggaggagcca
     1321 tgtcagaacg acctgatgct gctcagttgg cagaagcgat caagggactc tcggatgagg
     1381 acctaaaagt ggaactcgag aagtggggca tcgacacggt gctcaaggac atcttcgcag
     1441 ggatgcagga cgcattccgg cccgacaaag ccggctcgaa tgcggccctc atccagtacg
     1501 acatcgatgt cgacggcgac atccgttcat ggacggtcga tatcgcaaac ggtgcctgca
     1561 agacatccga gggggccgct gagaacccca ggctcacgct ccagctgggc ctcatcaact
     1621 tcgtgagact catcttccgt caggccgatg gcacgcaact cttcatgagc gggaagctga
     1681 agctcaaggg cgacatgatg ttcgcgatgc agatgcagaa ctacttcgaa tctccggcct
     1741 aggtcgtctc tttggaaagc tgcatcatca cggccgctct gtcgggcgta gcggcggatc
     1801 gttcgcagtg tcctgcgatc ccatacacgc ccgatgagta cgccgccgaa gccaaacgcg
     1861 cccgcgacgc gggggccgcg gtcgttcacc ttcacgctcg gcatgcggat gggagtccgt
     1921 catatcgagt cgaggagtac cgcgccataa ccgaagccat caaggcatcg gcccccgacc
     1981 taatcatcaa ctactcgacg ggcgcggttg gcatcccgaa agaggagcgg atcgcgcaca
     2041 tcgttgacct tcggcccgag atcggcgctc tcaacatggg ttcgatgacc tacgcgaagt
     2101 actcatccaa acgcaagcag atgatcttcg acttcgtctt cgagaatcgc ttcgagacca
     2161 tcgtctactt cctcgagaag atgaatgagg tcggcgtgaa gcccgagtgt gagtgtttcg
     2221 atacgggcca catccacaac gcgattccgc ttctcgacat ggggcttctc acggcccccg
     2281 tgcagttctc gctcatcctc ggtgtgctcg ggggggcccc cgcagacgcg cgaacgctcg
     2341 cccacatggc atcgctcgtt ccgcagccgg ctacctggga ggtcatcggt atctctcgtg
     2401 atcaatggat gcttgtgacg gcggccgtgg ctctcggcgg caatgtgcgc gtcggtctcg
     2461 aggacaactt ctacaaacct tcgggcgaga tggcatcttc gaatggggac ctcgtcgcgg
     2521 ccgcagctca gatcgtgcaa ctcagtggcc gcaaggttgc cgagcccgat gaggcccgcg
     2581 ggctcctcga cctcaagatc ccgcaggggc tctgagtggg agaggttctc agcgagatgg
     2641 tcgccaacgt t
//
LOCUS       DATDOZ010000038         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3688848, whole genome shotgun sequence.
ACCESSION   DATDOZ010000038 DATDOZ010000000
VERSION     DATDOZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688848"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1101
                     /gene="hisC"
                     /locus_tag="VI541_00710"
     CDS             10..1101
                     /gene="hisC"
                     /locus_tag="VI541_00710"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLF69459.1"
                     /translation="MSDPELRERIGGRSDLADLQPYRAPQMDAPVKLNTNESPYPPPE
                     QFIERLSAKLAETSLNRYPDRDFTEVRESLAAHVGTLTDRIWVANGSNELLLQLLLAF
                     GGGERKAMTFEPTYAMHSHIIRISGTRMLRGRRLPDYSLHIDATLEAIRVQRPDVVFL
                     CSPNNPTGNIDSEEAVSAICDVAPGLVILDEAYGEFSGSTLIRLVEEHDHLVVTRSFS
                     KAWRLAGARVGYLVGQPWTIEELLKVRLPYHLSSLAQAVVTSALSMAGEMLSTVETIR
                     HERDRLWGELSTTRGLKAFPSYANFILFRCESRPASEVWQDLLASGVLVRDFSDVVGC
                     EECLRVSVGTPDEDERFLEALSLSLAKSR"
     gene            1098..1691
                     /gene="hisB"
                     /locus_tag="VI541_00715"
     CDS             1098..1691
                     /gene="hisB"
                     /locus_tag="VI541_00715"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HLF69460.1"
                     /translation="MSNRTSVIERSTTETRVRVSIDLDGSGTTDISTGVGFFDHMLEQ
                     LGRHSGCDIEVKAQGDLHIDSHHTVEDVGIVLGAALVEALGDKAGIRRYGWSLVPMEE
                     ALAQVALDLSGRPFLAFDVEIPAETIGNYDPDLTQEFFTALTRSAGITLHVKLLAGTN
                     SHHIVEAVFKGVAQALSAAVALDPRRADSVPSTKGSL"
     gene            1688..2284
                     /gene="hisH"
                     /locus_tag="VI541_00720"
     CDS             1688..2284
                     /gene="hisH"
                     /locus_tag="VI541_00720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HLF69461.1"
                     /translation="MKVGVVDYRMGNLASVSKALERVGARAFVSDDKQELATSDLLVL
                     PGVGNFTAGMANLKQFNLIDPVSEWARDGRPLLGICLGMQLLFDSSQEGESEGLGILT
                     GDVVRLAASVKVPHMGWNSISARGSEIFEGSDGRSFYFVHSYVCRPETSTVGAVTEYG
                     DGFASGVHIGSICGVQFHPEKSSHDGLALLAKIVEVLV"
     gene            2281..3006
                     /gene="hisA"
                     /locus_tag="VI541_00725"
     CDS             2281..3006
                     /gene="hisA"
                     /locus_tag="VI541_00725"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HLF69462.1"
                     /translation="MTEIIVAIDIVEDQVVRLRQGELLERTVYGDDPIETAIRWEAQG
                     ATRLHVIDLDGATRGDASNSEAVERIISAVRIPVQVGGGIRSIDSIERWLEKGAARIC
                     VGTRSLDPTFLKEAIEVAGDRLIVAIDSRGGEVKVAGWLEGTGTRALDLAKQVESAGV
                     SRIIFTDIERDGTLLGPNVEAIDELLSEVGIPVIASGGVSSDIDVMALAKLHERGLEG
                     IIIGKALYSGALTLEVAQAAAEG"
BASE COUNT          594 a          879 c          984 g          613 t
ORIGIN      
        1 atgtagcgga tgagtgaccc agagctgaga gaacgtatcg ggggcagatc ggatctagcc
       61 gacctgcagc cataccgcgc gcctcagatg gatgctccgg tcaagctgaa cacgaacgag
      121 agtccttacc cgccacctga acagttcatc gagaggttgt ccgccaaact ggcagagaca
      181 tctcttaacc gttatccgga ccgcgacttc acggaggtga gggagagcct cgccgctcat
      241 gttggtacgc tcaccgatcg tatctgggtc gcgaacgggt cgaacgagtt actgctccaa
      301 ctcctcctcg ccttcggcgg gggcgagcgc aaggcgatga ccttcgagcc gacctatgcg
      361 atgcactcgc acatcatccg catctcggga accaggatgc ttcggggacg caggcttccc
      421 gactactcgc tgcatatcga cgcaactctt gaggccatca gggttcagcg ccccgacgtg
      481 gtctttctct gctctccgaa caatccgacg gggaacatcg atagcgagga agccgtctcc
      541 gcgatatgcg atgtcgctcc cggattggtc atcctcgacg aggcatacgg cgagttctcg
      601 ggctcgacct tgatcaggct ggtcgaagaa cacgaccacc tggtggttac taggagcttt
      661 tcgaaagcct ggcggctcgc gggtgcgcgg gtcggatatc tggtcggaca accctggacc
      721 atcgaagagc tgctcaaagt gcggctgccc tatcacctct catctctggc tcaggctgtt
      781 gtgacatcag cgctctcgat ggctggggag atgctcagca ctgtcgagac catccggcac
      841 gaacgtgatc gactctgggg tgaactgtct acgaccagag ggctgaaagc atttccttcg
      901 tacgccaact tcatcctctt cagatgcgag tcccggcccg cgagtgaggt ctggcaggac
      961 ctgctcgcgt ctggggttct ggtgcgtgac ttctccgatg tcgtcggatg cgaggagtgt
     1021 cttcgggtct ctgtaggcac ccctgacgag gacgagcgat tcctcgaagc actctcactc
     1081 agtctggcga aatctcgatg agcaacagga cgagcgtgat cgagaggagt accaccgaga
     1141 cccgcgtccg ggtgagcatc gacctcgacg ggtcagggac caccgatatc tcgacaggcg
     1201 tcgggttctt cgatcacatg ctggaacagt tgggacgcca ctcgggatgc gatatcgaag
     1261 tcaaggcgca aggcgatctg cacatcgact cgcaccacac cgtcgaggat gtcgggatcg
     1321 tcctgggtgc cgcattggtc gaggctcttg gtgacaaagc cggtatccga aggtacgggt
     1381 ggtccctggt acccatggag gaagctttgg ctcaggtagc gctcgacctg agcggtcggc
     1441 cctttctagc attcgatgtc gagataccag cggagaccat cggcaactac gaccccgacc
     1501 tcacgcagga gttcttcacc gcgcttaccc gctcggcggg catcacgctc cacgtcaaac
     1561 tccttgctgg cacgaactcc caccacatag tcgaagccgt attcaagggg gtagctcaag
     1621 cattgtctgc cgccgtggcg ttggatccgc gaagggccga ctcggtgccc tcgacgaagg
     1681 ggagcctttg aaggtcggtg tcgttgacta tcgcatgggc aaccttgcga gcgtgtcgaa
     1741 ggccctcgag cgagtaggtg cgagggcctt cgtttctgac gacaaacaag agctggcgac
     1801 ctctgacctg ctggtactgc ccggtgtcgg caacttcact gctggtatgg cgaacctgaa
     1861 gcaattcaac ctcatcgacc cggtatcgga gtgggcgcga gatggccgtc cacttcttgg
     1921 tatctgcctg gggatgcaac tcctcttcga ttcgtcacaa gagggagaga gtgagggcct
     1981 tggcatcctg actggagatg tcgttcggct cgcggcctcg gtcaaggttc cgcacatggg
     2041 ttggaactcg atctccgcca ggggttcaga gatattcgaa ggctcggatg ggcggagctt
     2101 ctatttcgtt cactcatacg tctgccgacc agagacctcg actgtggggg ccgtgacgga
     2161 gtacggcgat gggttcgctt caggagtgca catcgggagt atctgcgggg ttcagttcca
     2221 tccggagaag agcagccatg acggcctggc tctgctcgca aagatcgtgg aggtgctcgt
     2281 atgaccgaga tcatcgtggc gatagatatc gttgaggatc aggtggttcg tctgcgtcag
     2341 ggcgaattgc tcgagagaac cgtctacggc gacgacccca tcgagacggc gatccggtgg
     2401 gaggctcagg gcgccacgcg gcttcatgtg atcgacctcg acggagccac tcgcggcgac
     2461 gccagtaact ccgaggccgt cgagaggatc atctcggcgg ttcggatccc ggttcaggtt
     2521 ggagggggca tccgctcgat cgattcaatc gagagatggc tcgaaaaggg cgcggcgcgc
     2581 atctgcgtgg gtacgcgcag tctcgatcca acgttcctga aggaggccat cgaggtcgcc
     2641 ggagatcgtc tgatagtggc gatcgattcg cgaggcggcg aagtgaaggt ggcaggttgg
     2701 ctcgagggca cgggcaccag ggccctcgat ctcgcgaaac aggtcgagtc cgcgggggtg
     2761 tcgcggatca tcttcaccga tatcgagagg gatgggacac tcctcggccc caacgtcgag
     2821 gcgatcgatg aactcttgag cgaggtgggg atacctgtga tcgcctccgg gggggtctcg
     2881 agtgacatcg atgtgatggc gcttgccaaa cttcacgagc ggggcctcga ggggatcatc
     2941 atcggcaagg ccctctactc gggggccctc accctcgaag tcgctcaagc cgcagccgag
     3001 ggctgaccgt tgctctgcaa gcgtgtcatc ccatgtctcg atgtcgatcg cgggcgcgtg
     3061 gtcaaggggg
//
LOCUS       DATDOZ010000039         4320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2124114, whole genome shotgun sequence.
ACCESSION   DATDOZ010000039 DATDOZ010000000
VERSION     DATDOZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124114"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VI541_00730"
     CDS             <1..1314
                     /locus_tag="VI541_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLF69463.1"
                     /translation="SDIRQDEDVLDTWFSSQLWPFSTLGWPERTHELEYFYPTSVIVP
                     GYDIIHLWVSRMIMSGLHFMGDVPFRHALIHGIVRDADGKKMSKSAGNVRDPLDLMDK
                     YGTDALRFTLAEHATGQDIFLHEEWLGGSRNFSNKLWNAARFVLSDLRDEGSIELPPR
                     TRWTLADRWIMSRLERLTDEVTEMIEGFEISAAARSLYEFTWSSYCDWYIEAAKPRLY
                     SKDEGSRSDVVAILVHVLTRTIKLLHPIMPFITEEIWSYLPGAQGHLIVAEWPSADMQ
                     ATSLIDEQRFESVQEVVTALRRFRANHGIPLKTELPVGVHGQGDGFAQLLGVKADLSS
                     LARHCLFDFEPAAETKGPNARLVAGGVELRIPLEGLLDLGAERTRLTKALSDAASEID
                     RIETKLGNPRFVDKAPAPVVEEHRRRLDESLELRTKLAAQLEELN"
     gene            1259..2632
                     /locus_tag="VI541_00735"
     CDS             1259..2632
                     /locus_tag="VI541_00735"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HLF69464.1"
                     /translation="MNHWNFERSWQLNSKNSTEMDAYRRAIKALDRRGFGIRPGLDRV
                     RALLELLDDPQKSYPSIHVAGTNGKTTTSRIIGALLAAHGCSVGTYTSPHLQSVRERF
                     ERLGSAEEGIVADLIEPEEFALLHEYLGPFIATIENERGETVTYFEVLTAMAFDWMAQ
                     QAVGAGVFETGLGGRWDATNVLEPQVCVLTNIDVDHAIFLGSTPVENAREKAGIIKPG
                     TVVVSAQQDPEVDPVITEAALREGAELHRMQDSFEILSDDVAEGGRITTVRGLHSTYE
                     DLFLPLMGQHQSRNLAVSIAAVEAFFGRQLDPEATRAALLQVRSPGRMEIVRLHPVVM
                     LDGAHNPDGAATLAGAIKTSFPGRHTTLVLSIMSDKDREGILERILPLADSVIFTRCE
                     GPRASDPRDLASIASKLGHASSVVPSLREAIDAAIDQSGHEDLVLMTGSLYAVGEARD
                     HLVGPIE"
     gene            2654..3061
                     /gene="ndk"
                     /locus_tag="VI541_00740"
     CDS             2654..3061
                     /gene="ndk"
                     /locus_tag="VI541_00740"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLF69465.1"
                     /translation="METTLVLVKPDGVRRGLIGEVIGRIERKKLRVSALRMLTIDDAL
                     ARKHYEEHLDKPFFGDLLAFITSGPVVAMAVEGEEAISVVRTLMGATDPKKATPGTLR
                     GDLGLEVTENIVHGSDGPESARRELALYFPELS"
     gene            3173..4210
                     /locus_tag="VI541_00745"
     CDS             3173..4210
                     /locus_tag="VI541_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLF69466.1"
                     /translation="MPVDKAFTFMGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINT
                     KTNAILAVGAEAKRMIGRTPSNIQAVRPLKDGVIADFDVTEKMLRYFIQKVHKRRVLA
                     KPRVVVCVPSGITGVEQRAVEDATIAAGARSAFIIEEPMAAAIGAGLPVHEPAGNMVV
                     DIGGGTTEVAVISLGGIVTSLSIRIGGDELDESIIAYIKKEYSLMLGERTSEEIKMTI
                     GSAFPLPDEPHAEIRGRDLVTGLPKTIIVSSEEIRRAIEEPVHAIVDSVKATLDKTPP
                     ELAADIMDKGIVLTGGGALLKGLDERLKHETGMPIHVTENPLSCVALGSGKCLEEFEA
                     LKRVLIASSRR"
BASE COUNT          892 a         1239 c         1317 g          872 t
ORIGIN      
        1 agcgatatac gtcaagacga ggatgttctc gacacctggt tctcgagcca gctctggcct
       61 ttctccactc tggggtggcc cgaacgcacg catgaactcg aatacttcta tccgacgtcg
      121 gtgatcgttc ctggctacga catcatccat ctctgggtct cgcgcatgat catgtcgggc
      181 ctccacttca tgggagacgt tccttttcga catgccctca tccatgggat cgtccgtgac
      241 gctgatggga agaagatgtc aaagtccgcg ggtaacgtac gcgatcccct cgacctcatg
      301 gacaagtacg ggaccgatgc attgaggttc actctcgccg aacatgccac gggtcaagac
      361 atcttccttc acgaggaatg gctgggaggg tcgcgcaact tctccaacaa gctttggaac
      421 gcggcacgat tcgtcttgtc ggatctgagg gacgaaggct cgatcgaact tcctccgagg
      481 acgagatgga cactggcaga tcgctggata atgtcccgac tcgaacgact cacggatgag
      541 gtcaccgaga tgatcgaagg attcgagatc tcggctgccg cgcggtcgct ctatgagttc
      601 acctggagtt catactgcga ctggtacatc gaagctgcga agccgcgtct ttactccaag
      661 gatgaagggt cccgctccga cgttgtggcg atcctggtcc atgtgctgac caggacgatc
      721 aaactactcc acccgatcat gcccttcatc accgaagaga tctggagcta tctgcctggc
      781 gcgcagggcc acctcatcgt ggccgagtgg cccagtgccg acatgcaggc gacctcgctg
      841 atcgacgagc agcgtttcga gagcgtgcag gaggtcgtca ctgccctccg gcgattcagg
      901 gcgaatcatg ggatacctct gaagaccgaa cttccggtcg gggtccatgg tcaaggcgat
      961 ggtttcgcac aacttctcgg agtcaaggcc gacctgtcgt ccttggccag acactgcctg
     1021 ttcgatttcg aaccggctgc cgagacaaag ggacccaatg cccgtctcgt tgctggtgga
     1081 gtcgaactaa ggatcccgct cgaaggtctt cttgatctgg gggccgaacg gacacgtcta
     1141 acgaaggcgt tgtcggacgc agcgtcagag atcgacagga tcgagacaaa gctcggcaat
     1201 cctcgcttcg tcgataaggc tccggccccc gtggtcgagg agcaccggcg gcgactcgat
     1261 gaatcactgg aacttcgaac gaagctggca gctcaactcg aagaactcaa ctgagatgga
     1321 cgcataccga agggctatca aggccctcga tcgacgcggg ttcgggatcc ggcctggcct
     1381 agaccgcgtg cgagctctgc tcgaactgct cgacgacccg cagaagagtt atccatcgat
     1441 ccatgtggct ggtacgaatg gtaagacgac aacttcgcgc atcatcgggg cccttcttgc
     1501 tgcgcatgga tgcagcgttg gaacgtacac gtctccgcac ctgcagtcgg ttcgagagag
     1561 gttcgagcgc ttggggtcgg cggaagaggg gatcgttgcg gatctgatcg agccagagga
     1621 gttcgcgcta ctgcacgagt atctgggccc gttcatcgcc acgatcgaga acgagcgcgg
     1681 tgagacggtc acctacttcg aagtgctcac tgcgatggcc ttcgattgga tggcccaaca
     1741 ggccgtgggg gccggtgtct tcgagaccgg tctaggcggc cgatgggatg caacgaatgt
     1801 gctcgagccg caagtctgtg tcctaacgaa tatcgatgta gatcacgcga tcttcctcgg
     1861 gtcgaccccc gtggagaatg cccgcgagaa ggcagggatc atcaagccgg gtaccgttgt
     1921 cgtctcagcc caacaggacc ccgaggtgga cccggtcatc accgaagcgg cgctccggga
     1981 gggcgctgag ctacatcgca tgcaagattc cttcgagatc ctctcggatg acgtagcgga
     2041 gggaggccgg atcacgaccg tccgcggact ccattcgacc tacgaagacc tgttccttcc
     2101 cttgatgggc cagcatcaat cacgcaacct cgcagtgtcg atagcagccg tcgaggcgtt
     2161 cttcggacgt cagctcgatc ccgaggctac gcgagccgcc ctccttcagg tgaggtcccc
     2221 aggtcggatg gagatcgtac ggttgcatcc cgtggttatg ctggatggcg cccacaaccc
     2281 cgacggggca gcgacgctgg caggtgccat caagacatcc tttcccggaa gacacacgac
     2341 tttggtcctc tcgatcatgt cggacaagga tcgggaaggg atcctcgagc ggatactgcc
     2401 cctggctgac tcggtcatct tcacgcgttg cgaggggccg cgagcgagcg acccacgaga
     2461 cctagcatcg atcgcctcga aactcggtca cgcgtcgtcg gtggttccct cccttcgtga
     2521 ggcgatcgac gccgcgatcg accagtcggg gcatgaagac cttgtcctga tgacaggctc
     2581 tctctacgcc gttggcgagg cgcgtgacca tctggtcggt cccatcgagt gacatcgact
     2641 atccttgccc gtcatggaaa ccacactcgt tctcgttaag ccagacggag tcaggcgcgg
     2701 tctgatcggc gaggtcatag gcaggatcga gcgtaagaaa cttagggtca gcgccctgcg
     2761 gatgctcact atcgacgatg ctcttgctcg caagcattac gaggagcatc tcgataagcc
     2821 gttcttcggg gatcttcttg ctttcatcac ttcgggcccg gttgtcgcca tggctgtcga
     2881 gggcgaagag gcgatcagcg tcgttcgaac gctcatgggg gctaccgacc ccaagaaggc
     2941 aacacccggc acactcaggg gcgatctggg gctagaggtg accgagaaca tcgtccacgg
     3001 ttcggacggt cctgagtcag ccaggcgaga gttggccctg tatttccccg agttgagcta
     3061 gttggagctc gctcatctgc acgcttgcgg tttgcccggt acgcttcggt aggattcaat
     3121 gtgttgttca ccttgaacct gatccgctgg gttcggtcgt ctaggagtct caatgccggt
     3181 tgacaaagct tttactttca tgggccgcga catggcggtg gaccttggga ccgccaacac
     3241 cctggtctat gtgcgcggca gagggatcgt cttgaacgag ccctcagtgg tcgctatcaa
     3301 caccaagacg aatgcgatcc tcgcagtggg ggccgaggca aagcggatga tcggccgcac
     3361 gcccagcaac atccaggcgg tcaggccctt gaaggacggc gtcatcgccg acttcgatgt
     3421 gaccgagaag atgctccgct acttcatcca gaaggtgcac aagcgtcgag ttctggcgaa
     3481 gccgcgcgtc gtggtctgcg tgccatcagg gatcacaggg gtcgagcagc gtgcggtcga
     3541 agacgccacc atcgctgcag gggcccgcag tgcgttcatc atcgaggaac ccatggctgc
     3601 agcgatcgga gctggcctgc cggtacatga accggcaggg aacatggtcg tcgatatcgg
     3661 tggcggaacc actgaagttg ccgtcatctc actaggtggc atcgtcacca gtctttcgat
     3721 acggataggt ggcgatgagt tagatgaatc gatcatcgct tacatcaaga aggagtactc
     3781 cttgatgctc ggtgagagaa cgtcggaaga gattaagatg acgatcggct ccgcgttccc
     3841 tctgccggat gaaccacatg cagagatacg cggccgggat cttgtcaccg gacttcccaa
     3901 gacgatcatc gtctcctccg aggagatccg cagagctatc gaggaaccgg ttcacgcgat
     3961 agtggactcg gtgaaggcaa cattagataa gacaccaccg gagttggccg cagacatcat
     4021 ggacaagggg atcgtcctga cgggtggcgg cgcactcctg aaaggattgg atgagagact
     4081 caagcatgag accgggatgc caatacatgt cacagagaac cctctgtcct gtgtggcgct
     4141 cgggtctggt aagtgcttgg aagagttcga ggccctcaag cgggtactga tagcctcaag
     4201 ccgtcgttga ggtttagccc ccacgggcgc tatccatcgg gcggtcgctc gtcgaccgtg
     4261 atccccgtat cgataggggt tttttctgca gatgcatcga agatcaagta ggcagcggat
//
LOCUS       DATDOZ010000040         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_288419, whole genome shotgun sequence.
ACCESSION   DATDOZ010000040 DATDOZ010000000
VERSION     DATDOZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288419"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VI541_00750"
     CDS             <1..559
                     /locus_tag="VI541_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLF69467.1"
                     /translation="SPDYLSLAVLSSVIGGSANSRLSNRVREEKGYTYGIGGGFDFRR
                     FAGPFRVSTPVKTAITVDAIREIIDVLDDVRENGITAKELEEAKGFLAGLHILRFEGS
                     ASIAGMLASQIVHDLPEDYNDTFRDQVWALDLDEVNAATRYLRENRAIVLVGDAEEIR
                     DDMVSADFGPVAIVEDPEPGQPPDA"
     gene            complement(577..1362)
                     /locus_tag="VI541_00755"
     CDS             complement(577..1362)
                     /locus_tag="VI541_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69468.1"
                     /translation="MNRIFLVAGATIREHSRRRTLIFFGVVSALVTAGSIYFVLTRPD
                     LASLLGANLTLPELTSLGLFSLLATIVTLVISMGNIRPLFASGDADAMLSRPITRTDL
                     VAGRTLGSVMLVIGSCFAFGAASQIISMIAGEGLSMDLVGHWVSEAFNLIVLCLMTTL
                     LSGWFKAPAIVAVCGYLADQVVTSIQTFYQMVESGTLAGTVAKVIKMAWWVSPKYLPS
                     PLLEKTGLVGQPSLQWTLVLWAAGWSALLMFFIVKKASHSEAF"
     gene            complement(1359..2069)
                     /locus_tag="VI541_00760"
     CDS             complement(1359..2069)
                     /locus_tag="VI541_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69469.1"
                     /translation="MKALTARQVSKAYPGGEQALDGIDVEVAEGEAFGLLGPNGAGKS
                     TFVRCALDLIRPTSGEIQLFGVASGDREARVGVGSALESPRYPGYLTGAEFMRLQSQL
                     AGIVPDSAHIAALLDRASLSPEQKIGTFSKGMLRQLAIAQALIGNPRLLLLDEPTADL
                     DPIGRRQVRDRLHELKQQGVTVVLNSHLLSEVERLCDRVAIMHQGKIIASGQLDTLVP
                     EGSDLETVFIDLITKAQA"
BASE COUNT          465 a          631 c          662 g          424 t
ORIGIN      
        1 gtccccggac tacctgtcgc tagctgtcct ttcctcggtc atcggtggct cagccaactc
       61 gcgtctcagc aatagggtta gagaagagaa gggatacaca tacgggatcg gcggagggtt
      121 cgacttccgc aggttcgccg gacctttccg tgtgagcact ccagtcaaga ccgcgatcac
      181 tgtggatgcc atcagagaga tcatcgacgt tctggatgac gttcgcgaga atggcataac
      241 tgccaaggag ctagaagagg ccaaggggtt cctcgccgga ttgcacattc tgcgcttcga
      301 agggtctgcc tcgatcgcag ggatgctggc ctcgcagata gtgcacgatc tgccagagga
      361 ctacaacgac acgttccgcg atcaggtttg ggctcttgat ctggacgagg tcaacgctgc
      421 gaccaggtat cttcgtgaga accgcgcgat cgtacttgtc ggagacgccg aggagatcag
      481 agacgacatg gttagcgcgg acttcggacc cgttgcgatc gtggaagacc cagagccagg
      541 gcagccgcca gacgcctagg gagggatcct tgatcgtcag aaggcctcgc tatgcgatgc
      601 cttcttgacg atgaagaaca tcagaagtgc tgaccaaccc gcggcccaga ggacaagggt
      661 ccactgcaag ctgggctgac caactaaccc ggtcttttcg agcagtgggg atggcagata
      721 cttcgggctg acccaccaag ccatcttgat gacctttgca accgttcccg ccaaggtgcc
      781 gctttcgacc atctgataga aggtttggat gcttgtcacc acctggtcgg ccagatagcc
      841 gcacacggcg acgatcgcag gggccttgaa ccaaccagat agcagcgtcg tcatcaggca
      901 aaggacaatg aggttgaatg cctccgagac ccagtggccg acgaggtcca tcgacaatcc
      961 ctcgccggcg atcatcgaga tgatctggga ggcggccccg aatgcgaagc aggatccaat
     1021 caccaacatc acgcttccta gcgtccgtcc ggcaacaagg tccgtccttg tgatgggccg
     1081 cgacaacatc gcatcggcgt ctccgctagc gaatagtggg cggatgttgc ccatcgagat
     1141 cacgagcgtc acgatggtgg cgagcagact gaagagtccg agcgaggtta gttccgggag
     1201 cgtcaggttg gctcccagca gggaagccag atccgggcga gtgaggacga agtagataga
     1261 gcctgctgta accaacgcgg acacaacacc gaagaagatc agggttcgcc tacgtgagtg
     1321 ctctcggatg gtcgcccccg cgactaggaa gatgcggttc atgcctgtgc tttcgtgatc
     1381 aggtctatga atacggtctc cagatcactg ccttcaggaa caagcgtgtc cagttggcct
     1441 gaggcgatta tctttccctg gtgcatgatc gcgacccggt cgcagagtct ctcaacctca
     1501 gataacaagt gcgaattcaa gacaaccgtg acgccctgct gtttcagctc gtggagtctg
     1561 tctctgactt gtctgcggcc gatcggatcc aggtcagccg tcggctcatc gaggagaagc
     1621 agacgagggt tgccgatgag tgcctgtgcg atcgccagct gccggagcat ccccttagag
     1681 aacgtgccga tcttctgctc gggcgaaagg ctcgccctat cgagaagagc ggcgatgtga
     1741 gcgctatccg gaacgatccc ggcaagctga gactgcagtc gcatgaactc ggcccccgtg
     1801 agatagccgg ggtaccgagg ggattcgaga gcagatccaa cgccgacgcg ggcctcgcgg
     1861 tcaccagagg ccacgccgaa aagttgtatc tcgcctgatg ttgggcgtat caagtcgaga
     1921 gcgcagcgga cgaatgtcga cttcccagcg ccgttcgggc caagcagccc gaaggcttcg
     1981 ccctctgcta cttcaacgtc gatcccatcg agcgcctgct cgccgcccgg atatgccttc
     2041 gaaacctggc gggcggttag cgccttcaag cttaggcgag gtcttcgacg atgatcacat
     2101 cgaacgctgg cgcgaactgt ttggcatcct cggtggcgag cttggcctcc tccgagttca
     2161 tgtcggcctt catgccgtcc tt
//
LOCUS       DATDOZ010000041         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_288699, whole genome shotgun sequence.
ACCESSION   DATDOZ010000041 DATDOZ010000000
VERSION     DATDOZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288699"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VI541_00765"
     CDS             complement(<1..1179)
                     /locus_tag="VI541_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLF69470.1"
                     /translation="MSDPMSNGQVRLTIDDQVIDTLPGRMLIDVAEEHGVFVPRFCYH
                     PGLESVAACRMCLVEIEGGKRPLEPACATQIAQGMVVHTKSETAEAAQKAVLEMLLIN
                     HPLDCPICDRGGECPLQDQTMAFGPGTSRYMEEKRHFKKPLPISDLVLLDRERCVLCW
                     RCVRFCDEVSGDRFIDLMDRGSHTQINIAEEKPFDSYFSGNTIQICPVGALTSSSYRF
                     LSRPWDLKKSSTVCSHCSVGCPISLEHRDGEVLRAQALPNEEVNSFWNCDKGRFGHRY
                     VSHPERLRSPSVRQPSGMKPASWTVALDRIANTLKNISASSGPQAIGLIGGSRATNED
                     LYAAATFFRETIGTNNLDFRTFDADFPYEHLAVAGFAGSTASLNDLDDAGVILWVGPD
                     P"
     gene            complement(1176..1592)
                     /locus_tag="VI541_00770"
     CDS             complement(1176..1592)
                     /locus_tag="VI541_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69471.1"
                     /translation="MRAWRWLAAVFSVVGWVYAWYMFRGFRNAFARDEIPMPPWLLIA
                     MGLATTLLIATAVLIFMRSALWPRIAVVAGPVYLASLVIGQFAYMRGIFSSVTIKEAF
                     DTLFSGGGLAKVAFVVGVSLVLVLSGLTALIHREAT"
     gene            complement(1589..>2097)
                     /locus_tag="VI541_00775"
     CDS             complement(1589..>2097)
                     /locus_tag="VI541_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HLF69472.1"
                     /translation="IAGGLDEGTQFRAVIPGGASAPWLNTLDINMDFESLGAVGSMLG
                     SGSVIFMSDAICPVRMAWISTRFFNHESCGKCTPCREGSWWSVKILERIEKGEGRSED
                     LQTLLDVCDQMSAPGPTYVPKGQCFCALGDGQAWSLRSAISLFREEFEEHITAGACPL
                     DITAEVAS"
BASE COUNT          449 a          628 c          640 g          380 t
ORIGIN      
        1 tgggtctgga ccgacccaca ggatcacgcc ggcatcatcg agatcgttca gggaagccgt
       61 ggaaccggcg aagcccgcga ccgcgaggtg ttcgtacgga aagtcagcat cgaacgttcg
      121 gaagtcgagg ttgttcgtac cgatcgtctc gcggaagaag gttgcagccg cgtagaggtc
      181 ttcgttggtg gcgcgagaac caccgatgag ccctatcgct tgggggccgg agctcgccga
      241 gatgttcttg agtgtgttcg cgatccggtc gagcgccacg gtccacgacg ctggcttcat
      301 gccgctcggc tgacgaaccg atggcgaccg aagccgctcc gggtgcgaaa cgtagcgatg
      361 accgaaacgc cccttgtcgc agttccagaa cgagttgacc tcttcgttgg gaagtgcctg
      421 ggctctcagc acttcgccat cgcgatgttc gagtgagatg ggacacccga ccgagcagtg
      481 cgagcagacc gtcgatgact tcttgagatc ccacggcctt gataggaagc gataactgct
      541 cgacgttagg gccccgaccg gacagatctg gatcgtgttt cccgagaagt aggagtcgaa
      601 tggtttctcc tcggcgatat tgatctgggt atgacttccg cggtccatga gatcgatgaa
      661 cctgtcgccc gagacttcat cgcagaagcg gacgcaacgc caacagagta cacaacgctc
      721 gcggtcgaga agaacgaggt ccgagatcgg caacggcttc ttgaagtgac gcttctcttc
      781 catgtatctc gaagttcccg ggccaaacgc catcgtctga tcctgtagcg ggcactcgcc
      841 gccgcggtca cagatggggc agtcgagcgg gtggttgatg aggagcatct ccaacacggc
      901 cttctgtgcg gcctcggcgg tctcgctctt cgtatgaacg accatgccct gcgcgatctg
      961 tgttgcacac gcgggttcga ggggtcgttt gccaccttcg atctcgacga gacacatacg
     1021 gcatgcagcg accgactcga ggcctgggtg ataacagaac ctcggaacga atacgccatg
     1081 ctcctcggcg acgtctatga gcatccggcc aggtagcgtg tctatcacct ggtcatcgat
     1141 ggtcaatcgg acctgaccat tcgacatcgg gtcgctcatg tcgcttctct atggatgagg
     1201 gcggtgagcc ctgaaaggac gagcaccaaa gatacgccga ccacgaatgc caccttcgcg
     1261 agcccgcccc ccgagaagag cgtgtcgaag gcttctttga tcgtgaccga ggagaagatc
     1321 ccacgcatgt acgcgaactg accgatcacc agggatgcga gatagacggg cccggcgacg
     1381 acagcgatcc ggggccacag agccgaccgc atgaagatga gaacagcggt cgcgatcaac
     1441 agggtagtgg ctagccccat agctatcagg agccatgggg gcatggggat ctcatcacgg
     1501 gcgaatgcat tgcgaaaacc gcggaacatg taccacgcgt agacccaacc gaccacagag
     1561 aagacggcag cgagccatcg ccaggccctc atgaagccac ctccgcggtg atgtcgagag
     1621 ggcaggcccc cgcggtgatg tgctcttcga actcctctcg aaacagcgag atagccgacc
     1681 gaagtgacca agcctggcca tctccaaggg cgcagaagca ttggcccttg ggaacatatg
     1741 tcggacccgg agctgacatc tggtcgcaca catcgagaag tgtctggaga tcctctgaac
     1801 gtccctcgcc cttctcgatg cgctcgagga tcttcacgct ccaccaactg ccctctcggc
     1861 acggcgtgca cttcccgcag gactcgtggt tgaagaatcg agtcgagatc caagccatcc
     1921 tgacggggca gatcgcgtcg gacatgaaga tcaccgaacc agacccgagc atgctgccga
     1981 cggctccgag cgactcgaag tccatgttga tgtcgagcgt gttcagccaa ggcgcgcttg
     2041 ctcccccagg gatgacggcc cggaactggg taccttcgtc gaggccaccc gcgatcg
//
LOCUS       DATDOZ010000042         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1339467, whole genome shotgun sequence.
ACCESSION   DATDOZ010000042 DATDOZ010000000
VERSION     DATDOZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339467"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VI541_00780"
     CDS             complement(<1..802)
                     /locus_tag="VI541_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HLF69473.1"
                     /translation="MGFQRTDVEALSSALGEPGWVLDSRLAAWDTFSKLELPGEKDEP
                     WRYTDLRRLRFNLDAFKPIPPDPGREITDLQLELISQEQSRAGYVIQRDTDVVKVELN
                     ADLAKRGVILTDLGTAIREYPELVRPYLHSEADHSGHIFDALQVALSSGGTFLYVPRG
                     VTVELPIESQRWIDEPGSAVFPRTLVVAEEGAEVTYIERLRSTELESPSLCNAAVEVF
                     AGQASRVNLVTLQELGPQVWHFANNRYKTERDVNLRSLILTLGSRFSRN"
     gene            complement(803..1600)
                     /locus_tag="VI541_00785"
     CDS             complement(803..1600)
                     /locus_tag="VI541_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HLF69474.1"
                     /translation="MSVASSSARLDFPLDTRLLPYLSEDLANKARSVPAPKLRFHRLV
                     GRSQIELPDTVELQDVEVGEHLDSEYPTWDIEVEGTGNFVCEGFIVHNSKVTMKYPAV
                     ILAGEGAKAEILSVAYAGDGQHQDAGAKVTHAAPNTQSTIISKSISKDGGRAGYRGLV
                     RMDDGATHAKSNVRCDALILDAESRSDTYPYNEIEEQTGSVGHEATVSKVGDEQLFYL
                     QSRGIDESEAMGLIVRGFIEPITKELPLEYSVELNRLIQLQMEGSVG"
     gene            complement(1607..2581)
                     /locus_tag="VI541_00790"
                     /pseudo
     CDS             complement(1607..2581)
                     /locus_tag="VI541_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
     gene            complement(2558..3154)
                     /locus_tag="VI541_00795"
                     /pseudo
     CDS             complement(2558..3154)
                     /locus_tag="VI541_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
BASE COUNT          641 a          941 c          914 g          658 t
ORIGIN      
        1 cgttacgaga gaaacgcgaa ccaagggtca ggatgagact gcgcagattg acatcgcgct
       61 cggtcttgta gcggttgtta gcgaagtgcc acacctgggg gccgagctcc tgaagcgtaa
      121 ccaggttgac gcgcgaagcc tgcccggcga agacctcgac agcggcgttg cataggctcg
      181 gtgactcgag ctcggtagag cggagtcgct cgatatacgt gacctcggcc ccctcctcgg
      241 ccacgaccaa ggtgcgaggg aagaccgcag atcccggctc gtcgatccat ctctgactct
      301 cgatgggcag ctcgaccgtg acaccgcgcg gaacgtacag gaatgtgccg cccgaagata
      361 gggcgacctg cagcgcatcg aagatgtgac cgctgtgatc ggcctcggag tgaaggtacg
      421 gtcgcacgag ttcggggtac tcgcggatcg ccgtacccag gtcggtcaag atgacgccac
      481 gcttcgcgag atcggcgttg agttcgacct tcacgacatc ggtgtcgcgc tggatcacat
      541 agccggcgcg tgactgttcc tggctgatga gttcgagctg aagatcggtg atctcgcgac
      601 ccggatctgg cggtatcggc ttgaaggcat cgaggttgaa gcgcaacctt cgcagatccg
      661 tatagcgcca gggttcatcc ttctcgccgg gcagctcgag tttgctgaag gtatcccacg
      721 ccgcaaggcg cgagtcgaga acccagcccg gctcgccgag cgcgctgcta agagcctcta
      781 catcggttct ttgaaatccc acttaaccga ccgacccctc catctgcagc tgaatcagtc
      841 tgttgagctc gaccgaatac tcgagcggca gttccttcgt gatcggctcg atgaacccac
      901 gaactatcag ccccatcgcc tcggactcat cgatgccacg gctctgcagg tagaagagct
      961 gctcatcgcc gaccttcgat accgtcgcct cgtggccgac cgacccggtc tgctcctcga
     1021 tctcgttata gggataggtg tcggatcggc tctctgcatc gaggatgagc gcatcgcatc
     1081 ggacgttgct cttggcgtgc gttgcgccat catccatgcg aaccaggcca cggtagcctg
     1141 cccggccccc gtccttcgag atggacttcg agatgatggt cgactgcgtg ttgggggccg
     1201 catgcgtaac cttcgcaccg gcatcctgat gctgtccgtc accggcatat gcaactgaga
     1261 ggatctcggc cttggccccc tcgcccgcca ggatcaccgc gggatacttc atcgtgacct
     1321 ttgagttatg aacgatgaaa ccttcgcaaa cgaagtttcc ggtgccttcg acctcgatat
     1381 cccaggtcgg gtactcgcta tccaagtgct ccccaacctc gacatcttga agttcaacag
     1441 tatccggcaa ttcaatctgg gaacgaccca caagacgatg gaaacgcaac ttgggggcgg
     1501 ggactgaacg ggccttgttt gcaaggtcct cgctcaagta agggaggagt ctcgtgtcga
     1561 gagggaagtc gagtctcgct gaactcgagg caaccgacat ccgcccagat atccacacag
     1621 ggcgggggtc ctcatgctcc acgtagagtc tgctgtttat gcctagtccg gtcaggagat
     1681 ctgccacctg cctcagaaga tcactactgc acgacttaag tgagaatcgt cgtctggctg
     1741 caaccaaaca gccgtcggca tccagataac ctgctatgaa ggccagccgc tgcgacatcg
     1801 gaagtgagaa aacccacggc ggcactcgct tgttgtgtgc accacttgag aatccgttac
     1861 gttcgagcgc atccgcgagg tcgacgctgt tccaggaaag gtgcacgccg tcgctacgct
     1921 cagtaggggc agaagcactt atctccgtca tgaaggcgag cagttttgaa cgagctcggt
     1981 cagccttttg agtactgaag ccaaccttcg cccatcgcgt cacaccctcg gtctggcctg
     2041 gctcccggtc gatgtcccca tccccgagga aatagccgaa gaaccacatc acggcgggat
     2101 ccgtgatgac aggaaactca acagtggact ttctagttct ggtggcggtg agggttgatg
     2161 aatactggtt ggcggtttga tagtgcgttt cgatttcgct tggtggcgac caaagagaat
     2221 aagactgtcc gaactcgggt gacgtggttg gcactatcgc ccgcttcaaa tcatcagccc
     2281 gtacgtatgc gagatcgtag cgcccaagtt tcttgggccg tgacgggtcg tagtcgtatg
     2341 agtagaaggg atggttgtct gtcacgagta tcgagcgcga gccgctcttc acctgtcgga
     2401 ccggttgata gcccgagaac ttcttaccaa gtatctttcg gaagccgaac acacccttcg
     2461 actcatcgaa cgaaagaact tcctcgccta cttcaacgtc ctggatagcc ttcggccccg
     2521 ttggggtggt aactcgctca ccttgtgcga tacaccctaa gttgccatcg atccattcca
     2581 tcgttgcgtt cttctgcgcg accgccctct tcgtgaccag gttgtagacg ttgttcgacc
     2641 agttctggat ggtggtgtac cggcaccggg cgccctcttt gaccatgatc tcgaccactg
     2701 cggagtgcag tgagtcggac gagtagacgg gggccgagca gccctcgacg tagtggaccc
     2761 aagaaccggg ctcgcagatg atcaacgtgc gctcgaactg ccccatgttc tcctgattga
     2821 tccggaagta ggcctgcaga ggaacctcga ccgaaactcc ctcaggaacc cagatgaagg
     2881 aaccgccgga ccagacagcc gagttgaggg cggcgaactt gttgtcgttc tgcgggatga
     2941 tcgtgccgaa gtactcacgc accagatcct cgtgctcacg caacgcagtg tccatgtcgg
     3001 tgaagatgac gccacggtcc tccaactcct tcttggtgga gtggtatacc gactcgctct
     3061 cgtactgcgc ggacacgccc cccaggaagt tcttctctgc ctctgggatg ccgagctggg
     3121 gcgccgacct ctccgggatc gacttcgcga acat
//
LOCUS       DATDOZ010000043         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2384313, whole genome shotgun sequence.
ACCESSION   DATDOZ010000043 DATDOZ010000000
VERSION     DATDOZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384313"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VI541_00800"
     CDS             complement(<1..611)
                     /locus_tag="VI541_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69475.1"
                     /translation="MSPIGLELDDLQQEILRTVRDFVDREVIPVADEMEHRDEYPEKI
                     VEQMKEMGLFGVTIPEEFGGLGQPLTLYALIVAELSRGWMSLSGIMNTHFMTGYLIRK
                     HGTAEQKASYLPRLATGEVRAALSMSEPGAGSDVAAITCKAIRDGDHYVVDGQKMWVT
                     NGMRSGLITLLCKTDPSTEPAHKGISILLVEKTPDQEKPAPGLT"
     gene            complement(608..1558)
                     /locus_tag="VI541_00805"
     CDS             complement(608..1558)
                     /locus_tag="VI541_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLF69476.1"
                     /translation="MRARRSCHAVPGSSPKFLAKAPSLPADEIFFDLEDSVAPLAKEE
                     ARGNIVEALKNNDWGAKTRVLRINGVYTNWCYRDIIGVVEAAGRYLDCVMIPKVEYAS
                     DVVFVDKLLTMIEETVGLPNRIGLEVQIETATGLRNIYDIAHASPRMETLIFGPADMS
                     ASLGLPTVTAGLPMPGYPGDHWHWVLETILVAARDAGLQAIDGPYLLIDDLDGYREAA
                     RRARALGYDGKWSLHPGQIPILNEVFTPTQEEFDKAAAMLEAYGHATSVDLRGAVMFG
                     KEMIDEASRKMATQFVTRGEAAGLKPVKVWGDFKKEWGEA"
     gene            1643..>2357
                     /locus_tag="VI541_00810"
     CDS             1643..>2357
                     /locus_tag="VI541_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF69477.1"
                     /translation="MADPIELAAIERGAGDPVALVHGGVFHSGPAWAQSIGALAGAGY
                     RVIAVDRRGHGHSDAGDADFIPVHLHADDLRLTLELRDAKNVHLVGVSYGALVCLEFA
                     LSWPDRVSSMTLVEPALFAWVADDPDMGVWLERFDEIAASAAAGAPMTEWVPRWLMLI
                     DSKMAKEITPDSPSWSLIRKQGPLIFKEEPGWRYRPDRGLIRNLFVPTLVLNGDQSEP
                     PVQMIGEMLAEDLPLGTHEW"
BASE COUNT          443 a          708 c          715 g          491 t
ORIGIN      
        1 gtgagccctg gcgctggctt ctcctggtcc ggcgtctttt cgaccaggag gatgctgatg
       61 cccttatggg cgggctctgt tgacggatcc gtcttgcaga gcagtgtgat caaccccgag
      121 cgcatgccgt tagtgaccca catcttttga ccgtcaacga catagtgatc accgtcacgg
      181 atggccttac aggtgatcgc ggcgacatcc gacccggccc ccggctcgga catggaaagg
      241 gccgctcgga cctcacctgt tgccagcctc ggcaggtagc tcgccttctg ttcggcagtg
      301 ccgtgtttgc ggatgagata accagtcatg aagtgcgtgt tcatgatgcc ggagaggctc
      361 atccacccgc gagagagttc ggccacgatc aaggcataga gcgtgagcgg ctggccgagg
      421 cccccgaact cctctgggat agttacaccg aagaggccca tctccttcat ctgctcgact
      481 atcttctcgg ggtactcgtc gcgatgttcc atctcgtccg cgacggggat cacttcgcgg
      541 tcgacgaaat ctcttactgt ccgaaggatc tcctgctgaa gatcgtctaa ttcaaggccg
      601 atcggactca cgcctcgccc cactccttct tgaagtcacc ccagaccttg acgggtttca
      661 atccagcggc ctcgcctctt gttacgaact gagttgccat cttgcgcgag gcctcatcga
      721 tcatctcttt cccgaacatg accgcaccac gaaggtcgac cgaggtggcg tgtccgtatg
      781 cctcgagcat cgccgcggcc ttgtcgaact cctcctgggt tggggtgaac acctcgttga
      841 ggatagggat ctgacctggg tgaagcgacc acttgccgtc gtacccgagt gcacgagcgc
      901 gccgcgctgc ctcgcggtat ccatcgaggt cgtcgatgag caggtacggc ccgtcgatgg
      961 cttggagccc cgcgtcgcga gcggccacga ggatcgtttc gaggacccag tgccagtggt
     1021 cacccggata gccaggcatg gggagcccgg cagtcactgt cggcagaccc aacgaggcac
     1081 tcatgtcagc agggccgaag atgagggtct ccatccgggg tgacgcgtgg gctatgtcgt
     1141 agatgttgcg aagtccggtt gccgtctcga tctgaacctc cagaccgatc ctgttgggca
     1201 ggccaaccgt ctcctcgatc atggtgagca acttgtccac gaagacgacg tcgctggcgt
     1261 actcgacctt ggggatcatc acgcagtcca ggtatcggcc agcggcctca acgaccccga
     1321 tgatgtctcg gtagcaccaa ttcgtataga ccccgttgat ccgaagaacc ctggtcttcg
     1381 caccccagtc gttgttcttc aacgcctcga cgatgttccc ccgagcctct tccttggcca
     1441 ggggtgcaac cgaatcctcc aggtcgaaga agatctcgtc ggcgggaagt gatggggcct
     1501 tcgcgaggaa ctttggcgag gatccgggca cagcatggca ggaacgtctt gctctcatcg
     1561 gggatgcctt ccaggtaggg tgctcttgca agcaagatga tatcgaagtg gagcagtaga
     1621 ggctcttggt cgtgaggaac atatggcaga cccgatagaa ctggccgcga tagaacgcgg
     1681 agccggagat ccagtggccc tcgtgcatgg gggcgtgttt cactctggcc cggcctgggc
     1741 tcagagcata ggcgccctcg caggggccgg ataccgagtc atagccgttg accggcgtgg
     1801 gcatggccac tccgacgcgg gagatgcaga tttcatcccg gtgcatcttc acgccgacga
     1861 cttgcggctg actctcgaac tcagagacgc caaaaacgtt caccttgtgg gagtctcgta
     1921 cggtgcgctg gtatgtcttg agtttgctct gtcttggcct gaccgagtta gctcgatgac
     1981 gcttgtcgaa cctgctctat tcgcatgggt cgccgacgac cccgacatgg gtgtttggct
     2041 cgagcgattc gatgagatcg ccgcctctgc tgcagctggg gcccccatga cggagtgggt
     2101 accgcgttgg ctcatgctca tcgacagcaa gatggcgaag gagatcacgc cggactctcc
     2161 atcgtggagc ctcatccgca agcaagggcc actgatcttc aaggaggaac caggatggcg
     2221 atatcggccc gatcgaggtc tcatccgcaa cctcttcgtt ccaacacttg tgctgaatgg
     2281 agatcagagc gagccaccgg tacagatgat cggcgagatg ctggccgagg atctgcccct
     2341 cggtacgcac gagtgga
//
LOCUS       DATDOZ010000044         5778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2385752, whole genome shotgun sequence.
ACCESSION   DATDOZ010000044 DATDOZ010000000
VERSION     DATDOZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385752"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..250
                     /locus_tag="VI541_00815"
     tRNA            180..250
                     /locus_tag="VI541_00815"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:Gln,seq:ttg)
     gene            266..1639
                     /gene="glmU"
                     /locus_tag="VI541_00820"
     CDS             266..1639
                     /gene="glmU"
                     /locus_tag="VI541_00820"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLF69478.1"
                     /translation="MLISPLAVVVLAAGQGKRFHSDMPKVLHRMNGRTLIHHVISVVQ
                     SLGEVERLVVVVGRNADAVRTEVARHAPTAICVEQPEPLGTADAVKCALPLLDGFDGV
                     LVVLPGDTPLVSARSLISLIAYHADSSAAVSLLTARVDDPTGYGRVIQAANGHVTIVE
                     DADATDAQKSVDDISSGFWCFDISRLSDALGRIDASNEQGEYYLPDAAMAIGAGGGIV
                     TLQIEDPDEVRGINDREQLADAAAVARRRTISLLAAAGVTFEDPATTYVDADVEIGPD
                     TIVRPNTFLEGRTVIGARCSIGPSTRIVDSVVDDDAEVTFSVVREAKIGSGAQVGPFA
                     SVRPGTTLGPRSKVGTFVEVKASSIGEGSKVPHLSYIGDAELGADVNIGAGTITCNYD
                     GETGIKSKTVIGDGVLVGSDTMLVAPVNLADDSVTGAGSVVTKDVAEGDVVVGSPARP
                     VRRRRPR"
     gene            1681..2682
                     /locus_tag="VI541_00825"
     CDS             1681..2682
                     /locus_tag="VI541_00825"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HLF69479.1"
                     /translation="MEIGVKKRAMLYSGSSNPGLAEEIADHLGMRLGEVDLSRFASGE
                     VYVRFHDSTRGADVFVIQSHCEPVNDMIMEQLIMIDALKRASAKRINVVIPFYGYARQ
                     DRKSLAREPISARLVADLLRVAGADRILTVDLHSGQIQGFFDGPVDHLTALPILAQYV
                     SERYNENLVVVAPDAGRARHASKYAHHLRAPIAVMQKSRRADIRNSSSVLNVVGEVEG
                     RRCLIVDDMIDTAGTLTNAVAALLDQGATDVVAAATHGVFSPPAFERIESSALKEVIV
                     TNTLPQTRRSDKITVLSIAPIVAATVKAVFEDESVSEIFQGEHLEAIPPERRLDEDD"
     gene            2711..3361
                     /locus_tag="VI541_00830"
     CDS             2711..3361
                     /locus_tag="VI541_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HLF69480.1"
                     /translation="MEITLEAQTRLETGKGPARRARMSGGVPAVLYGYGVDATPLVVN
                     AKEMANAFNTEAGMNVLISLKLDGKQYLTVPREVHRHPVRGDYLHVDFVAVASDTKIT
                     AHIPVHVIGESRGIKEGGQLDQHLHELVVEALPADLLSAFEIDVTDLGIGDVVRVSDV
                     VAPSGVDITTDAELVVLGIIQPHELKVEAEGEAEAEAEAAAAAAAAPAEPTPETSE"
     gene            3398..3976
                     /gene="pth"
                     /locus_tag="VI541_00835"
     CDS             3398..3976
                     /gene="pth"
                     /locus_tag="VI541_00835"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLF69481.1"
                     /translation="MTDVWLIVGLGNPGPRYSDTPHNAGARTVELLASRLGGRFAMGK
                     ASALVADVRSGEHRLLIARPSTYMNISGEPVGRLARYLKVDGDHIVVVHDDIDLPLGQ
                     LRIKLGGGNAGHNGLSSIQRHLGTPDFYRVRIGVGRPPAGGQDPADYVLAKPGREAQE
                     AITQTQERAADAVLALIEDGLERTMNEFNTRS"
     gene            3973..>5778
                     /locus_tag="VI541_00840"
     CDS             3973..>5778
                     /locus_tag="VI541_00840"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLF69482.1"
                     /translation="MSAPLAALCGLWSGSSLLQRILEEGSCAVADVARAFTLAGLSAE
                     SSPLLVVLPRSRDAEVFADDLRQYLTSSDVVLYPAWEVLPGEAMSPTLSTMGMRIRVI
                     RDLRGGAGARVIVTSIRALLQRVSPGPIESIALEVGGSWSLDLLAEKLTGFGYERNYL
                     VERPGEFSIRGGILDVFPADGVEPVRADMFGDEIAELRSFGVSSQRSREPVETVEILP
                     CRELLLTDEVREKAATLTEKVKDQDTSSALSKIASGVLFPGMEAYLPIVSEDLVTISS
                     LLPSEGSVVILDPKQARDRAADFLSQATQWGGTVAAGHFVEFEEATSDVRTVEVWPYA
                     RGEEDPQVDVTGWDGFLGRVDDLAAELRGVHASGASLVVAAGRSSPRAREVLAQGGLG
                     LAEGPAAIGKAMLTDAHVMRGFKMMHGGIHLAIVGESDLFGKRRPVVASRAETPARAS
                     ALLLELASEDYVVHETYGVGMYHGMVTRDIGGVTRDYLIIEYHGDDRLYLPTEQLEAI
                     TRYTGGETPRLNRLGTAEWEKAKGRARKAVADIAGELIVLYSKRLNSPGHAFALDTPW
                     QRELEDSFIHVETPDQSRTIDEVKADMERPIPMDRL"
BASE COUNT         1128 a         1694 c         1860 g         1096 t
ORIGIN      
        1 cggcgatcgc gggcctctgc ctgaatgagg cgcatgcccg tgaagtggcc ggaggagctg
       61 cgatgagctt cgatcgggtc gaggtcgtta cctcggcccc cctgggggcc gaggtaacga
      121 ggatcacttg accggccgaa ccgtggcctt tataggaaac taatgaccca actgcccatt
      181 ccgggatggt gtaacggcag cactagggat tttgattccc tcagcgaagg ttcgaatcct
      241 tctcccggag catgcggata actccatgct gatctcaccc cttgccgtgg tggtgctcgc
      301 cgcgggccag ggcaagcgtt tccactccga catgccgaag gtcctccatc ggatgaatgg
      361 ccggaccctc atccaccatg tgatctcggt cgtgcagagc ctcggagagg tggagcggtt
      421 ggtggtggtc gttggccgga acgctgatgc tgtccgaacc gaggttgcac gccacgcccc
      481 gacggcgatc tgcgttgaac aaccagaacc gctcggcacc gccgatgcgg tgaagtgcgc
      541 gttgcctctg ctcgatggct tcgacggggt cctcgtggtt ctgccaggtg acacgcctct
      601 ggtctctgcg cggagcctga tctcactgat cgcctaccat gcagacagca gtgcggcggt
      661 gagtctgctc acggcgaggg ttgatgaccc cactggctac ggacgagtca tccaggccgc
      721 gaatggtcac gtcacgatcg tcgaggatgc cgacgccacg gatgcgcaga agagcgtgga
      781 tgacatctcg agcggcttct ggtgcttcga catctcgcgg ctctcggatg cgctcgggcg
      841 gatagatgca tcgaacgaac agggcgagta ctaccttccc gacgctgcta tggcgatcgg
      901 agccggtggg ggtatcgtga cgctacagat cgaagaccct gacgaggtga gaggtatcaa
      961 cgaccgcgaa cagttggcag atgccgcagc cgtagcgcga aggcggacga tctctctcct
     1021 agcggctgct ggcgtgacat tcgaggaccc cgctacgacg tatgtcgatg cggatgtcga
     1081 gatcggcccc gacaccatcg ttcgcccaaa cacattcctc gagggcagga ccgtgatagg
     1141 cgcgagatgc tccatcggac cgtcgacgcg gatcgtcgat tcagtggtcg atgacgatgc
     1201 cgaggtgacc ttctcggtgg tgagggaggc caagataggg agcggggcac aggtaggtcc
     1261 gttcgcttcc gtgcggcccg gcacaacgct cggcccccga tcgaaggtcg ggaccttcgt
     1321 cgaggtcaag gcatcgagca tcggcgaggg ctccaaggtg ccccacctgt catacatagg
     1381 tgatgcggag ttgggcgccg atgtgaatat cggtgcgggc acgatcacct gcaactacga
     1441 cggcgagacc ggcatcaagt ccaagaccgt catcggtgac ggggtcctgg ttggaagtga
     1501 cacgatgttg gtggcacccg tgaacctggc cgacgactcg gtaacgggag caggctcggt
     1561 cgtgacgaaa gacgtggcgg aaggcgatgt cgtggtcggg agtcccgccc ggcccgttcg
     1621 ccgtaggaga cccagatagc agtcgggtag cattaagacc ccgaacctaa ggaatggtcg
     1681 atggagatcg gtgtcaagaa gagggcgatg ctctactcgg ggtcatcgaa cccgggcctc
     1741 gctgaagaga tcgccgatca ccttgggatg cgcctcggcg aggtcgatct gagtcgcttc
     1801 gcgtcgggcg aggtctacgt caggtttcac gactcgacgc gaggcgccga tgtattcgtc
     1861 atccagagtc actgcgagcc cgtgaacgac atgatcatgg agcagctgat aatgatcgat
     1921 gctctcaagc gtgccagcgc gaaacggatc aacgtggtga tccctttcta cggttacgcc
     1981 aggcaggatc ggaagtctct ggcccgtgaa ccgatatcgg caaggttggt ggcggacctg
     2041 ctgcgcgtcg cgggtgctga tcgcatcctg accgtcgatc tgcacagcgg tcagatccaa
     2101 ggattcttcg atggacccgt cgaccaccta acggctctgc cgatactcgc ccagtacgtt
     2161 tcagagcgct acaacgagaa tctcgttgtg gtggcacccg atgcaggtcg cgctaggcat
     2221 gcgtcgaagt atgcccacca cctaagggcg ccgatcgccg tcatgcagaa gagccgtcgt
     2281 gctgacatcc gcaactcatc gtcggtactc aacgtggtcg gcgaggtcga gggtcgaagg
     2341 tgcctgatcg tggacgacat gatcgacacc gcagggacgc tcacaaatgc ggtcgcagct
     2401 ttgctcgacc agggtgcaac cgatgttgtg gcggcggcca cgcacggagt cttctcgccg
     2461 ccagccttcg agcggatcga gagctcagcc ctgaaggagg tgatcgtcac gaacacgctt
     2521 cctcagactc ggcgttcgga caagatcacc gtgctatcga tcgcacccat cgtcgctgca
     2581 acggtgaagg cggttttcga ggacgagtcc gtttcagaga tcttccaggg agagcacctg
     2641 gaagccatac cacccgaacg tcggctcgat gaggacgatt aggcctcgac ccgctacaat
     2701 ctgacggctc atggagataa ccctcgaagc acagacgcgt ctagagaccg gaaaaggtcc
     2761 tgcgcgtcgc gcgcgcatgt cagggggcgt ccccgcggtt ctctatgggt acggcgtaga
     2821 tgccactcct ctagttgtca acgccaaaga gatggccaac gcgttcaata ccgaggccgg
     2881 catgaacgtg cttatcagcc taaagctgga cggcaaacag tatctgactg tacccagaga
     2941 ggttcatcgc catccggtcc gcggtgacta tctgcatgtc gatttcgtgg cggtcgccag
     3001 cgatacgaag atcacggcgc atatccctgt tcatgtgatc ggtgagtcac gcggcataaa
     3061 ggagggcggg cagctcgacc agcatctaca cgagcttgtc gtcgaggccc ttccggccga
     3121 cctgctttcg gctttcgaga tagatgtcac ggatctaggc atcggtgatg tcgtccgcgt
     3181 ctcggatgtt gtcgcacctt cgggagtcga tatcaccaca gatgctgaac tcgttgtgct
     3241 cgggatcatc cagccgcatg agctgaaggt cgaggccgag ggcgaggcag aggcagaggc
     3301 cgaggccgcc gcagcagcag ccgcagcacc cgccgaacca accccagaga cttccgagta
     3361 gttacgagtc gtacgactcg tcatagtctc ttctgcgatg actgacgtct ggctgatcgt
     3421 gggtcttggt aaccccggcc cccgctattc ggacacaccc cacaacgcgg gtgctcgaac
     3481 cgtagaactc ctcgcgtcgc gcctcggtgg acgcttcgcc atgggcaagg cttcagcttt
     3541 ggtcgctgat gtgagatcgg gggaacaccg gctgctcatc gcacggccct cgacttacat
     3601 gaatatcagt ggtgagcctg tcggccgtct tgcacgttac ctcaaggtcg acggcgatca
     3661 catagtcgtc gtacatgacg atatcgacct tcctctcggt caactccgga tcaagctcgg
     3721 tggcggcaat gccggacaca atggattgag ttcgatccaa cgccacctcg gtacgccgga
     3781 tttctaccgt gtccgcatcg gagttggccg cccacctgca ggcggtcagg atccggctga
     3841 ctatgtactg gcgaaaccgg gacgcgaggc acaggaagcg atcactcaga ctcaggagcg
     3901 tgcggcggat gcagtacttg ccctcatcga agatggcctg gaacggacga tgaacgaatt
     3961 caacacgagg tcatgagcgc accccttgcg gccctatgtg gcctctggag cggatcatct
     4021 cttctgcagc ggatcctcga agaggggtca tgcgcggttg cggatgtggc tcgtgcattc
     4081 accctcgccg gtctatcggc agagtcttca ccgctgctcg tcgtgctgcc gcgaagccgc
     4141 gatgccgagg tcttcgcgga tgatctccgt cagtacctca catcttcaga cgtcgtgctc
     4201 tatcccgcct gggaggtact gccgggggag gccatgtctc ccacgctttc cacgatgggc
     4261 atgcgcatcc gagtcataag ggatctgcgt gggggggccg gggcgcgagt gatagtgacc
     4321 tcgatccgag cgctgcttca gagagtgagt cccggaccga tcgagtcgat cgcactcgag
     4381 gtgggcgggt cgtggtccct cgacctgctg gccgagaagc tcaccggttt cggatacgag
     4441 cgcaactacc tcgtcgagcg accaggcgag ttctcgataa gaggagggat cctcgatgtc
     4501 tttccggccg atggcgtcga gccggtgcgt gccgacatgt tcggcgatga gatcgcggaa
     4561 ctccgatcat tcggtgtttc gagtcagcgt agccgcgagc cggtcgagac cgtggagatc
     4621 cttccctgcc gcgagctgct tctcacagat gaggtacgtg agaaggcggc aacgctcacg
     4681 gagaaggtca aagatcagga cacatcctct gcgttgagca agatcgcatc tggagtgctc
     4741 tttccgggga tggaggccta cttgccgatc gttagcgagg acctggtcac gatctcttcc
     4801 ctcttacctt ccgaaggctc ggtagtcatc ctcgacccga agcaggcgcg cgaccgggcc
     4861 gcggacttcc tgtcgcaagc gacgcagtgg ggcggcacgg tggcggccgg tcacttcgtc
     4921 gagttcgaag aggcgacgtc ggatgtccgg acggtcgagg tttggcccta cgcacgcggg
     4981 gaagaggatc cccaagtcga tgtcaccggg tgggacggct tcctgggacg tgttgatgac
     5041 ctcgctgcgg aacttcgagg agtccatgca tcaggcgcga gcctggttgt cgccgcgggg
     5101 cgttcgtccc ctcgtgctcg cgaggttctc gctcaaggcg ggctcggcct agccgagggg
     5161 ccggccgcga tcggcaaggc catgctcaca gatgcccacg tgatgcgggg tttcaagatg
     5221 atgcatggag ggatacatct agcgatcgtc ggcgagagcg acctgttcgg caagcgcagg
     5281 ccagttgtcg cctcgcgtgc ggagacacca gcgcgtgcat cggccctcct gctggaactc
     5341 gcgagcgagg actatgtggt gcatgaaacc tacggggtag gcatgtacca cgggatggtt
     5401 acgcgggaca tcggtggcgt gacccgcgac tacctgatca tcgaatatca cggggacgac
     5461 aggctctacc tgcccaccga acaactcgaa gcgatcactc gttacaccgg tggggagact
     5521 ccgagactca accgactcgg aacggctgag tgggagaagg ccaaagggcg ggctcgcaag
     5581 gctgtcgccg acatcgcggg cgagttgata gtcctgtatt cgaagagact gaatagcccc
     5641 gggcatgcat tcgcgctcga taccccgtgg cagagggaac tcgaagactc gttcatccac
     5701 gtcgaaactc cagatcagag ccgcacgatc gatgaggtca aggccgacat ggagagaccg
     5761 atccccatgg atcggctc
//
LOCUS       DATDOZ010000045         3594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1863045, whole genome shotgun sequence.
ACCESSION   DATDOZ010000045 DATDOZ010000000
VERSION     DATDOZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863045"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VI541_00845"
     CDS             complement(<1..1024)
                     /locus_tag="VI541_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HLF69483.1"
                     /translation="MPAWQEPVHLICEQIPTDLPREDVLRSFRGDEDLVVLAGEWAGG
                     GALVASDPQHRASARDDPFLTMTTMPKVIGQVPGAIGGGWFGYLGYQLGGNIEQLPDP
                     PPRPTALPAFGLSYYDSLLRYDGTNDQWWFEALWSQDRAPVLERRLATIRTRLFGDPE
                     PPRRYTCTQFLACPDAARHREAVERCIEYIWAGDIYQANICMRLEADFTGDPFELYLS
                     GLLRLDPARSAYISGPWGAVASFSPELFLHRVGRQVRSAPIKGTGRRSSRPQDDEKQR
                     AALEASEKDRAENVMIVDLMRNDLGRVCAAGSVSVPSLISAEDHPGLWHLVSVVTGQL
                     HDGSDDT"
     gene            1158..1517
                     /locus_tag="VI541_00850"
     CDS             1158..1517
                     /locus_tag="VI541_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF69484.1"
                     /translation="MKMLTTGRVAASLGVTINTVKNWARAGKIQAVRLPSGHFRIPAT
                     EVDRLLQGLRSAGLREYEQWSRKQPSTPPNLDQVLTWNRQALEIARSQGKLPNDSLEE
                     KGERVRRMHRILAALSR"
     gene            1514..2083
                     /locus_tag="VI541_00855"
     CDS             1514..2083
                     /locus_tag="VI541_00855"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HLF69485.1"
                     /translation="MNDLEGAVVAIAMTLKANAVPYMVIGGFANLLWGEPRTTRDLDV
                     TVDIDLADWKEVDKLCQQIGRFLASDPQAMAERTRVVPLMTAGGVPVDLIIGMLTFES
                     EAIARARQVEFQGGLVSVCTPEDLVVHKIISERPRDLDDVVGILRRQGGAIDVPGLEA
                     LVSQLSTTMDDATIIDRFVEAKRLANLAP"
     gene            2139..>3594
                     /locus_tag="VI541_00860"
     CDS             2139..>3594
                     /locus_tag="VI541_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF69486.1"
                     /translation="MSTESGLETATVVFTDLEGSTDLRTRLGDELAHERIRIHEEIIR
                     EQISKFEGFEAKALGDGFMIVFGSARQAINCSVGIQIAIEEHNHSDPARAVKVRIGIN
                     SGEVTREASDVYGAAVNAAARICAKAQGGQILIPQVVKDLAGFVPDLRIVDRGLYWLK
                     GFPQRWRLHEVMWRGRGDEAVERADAPTIPRIRTDVPELPRALAPIVGRASELAAIDE
                     ELERVTTGTLRVLAFEGEAGIGKTRLLEAAAVAAKSKDLAVLRVGADEELRGPFFIFR
                     ALFATAEAEAVAEEAVALEALDRAKDALAGRDAGHQAGLSPQEQMLHVFDEVASAIRA
                     LASGSPLALLLDDVQWADEDSLRLLRYIARTIAASPILVLMTVREQVESPTGGTAHLL
                     ADLDRMHLARRVRLPRFSKKQTAELLRNLLGSPVAEQTLDMLHGRAEGVPFFVEEFAR
                     TYRESKALQQVGETWVMTQLTGPSVPASVQSLIER"
BASE COUNT          722 a         1037 c         1121 g          714 t
ORIGIN      
        1 ctgtgtcgtc ggatccgtcg tgaagctgac cggtgacgac agataccaga tgccatagcc
       61 ccgggtggtc ctcggcgctg ataagagagg gcacacttac agaacctgcg gcgcagaccc
      121 gacctaggtc gttgcgcatg agatctacga tcatcacgtt ttcggctcga tccttttcag
      181 aggcttcgag agccgcgcgc tgtttctcgt catcctgcgg acgggaactt cggcgacccg
      241 ttcccttgat gggtgcggat cgcacctgtc ggcccactcg atgcaggaag agttctggcg
      301 agaaacttgc gacagcaccc cacggcccgg agatgtaagc acttcgcgct ggatcgagcc
      361 ggagaaggcc cgataggtag agctcgaacg gatcgcctgt gaagtctgcc tccagacgca
      421 tgcaaatgtt ggcctggtat atgtcacctg cccatatgta ttcgatgcat ctctcgactg
      481 cttctctatg ccgtgcggca tcggggcagg ctagaaactg cgtgcatgtg tatcgccgcg
      541 gtggctcagg atcaccaaac aatctggttc ggatggtcgc aagccgtcgt tccaggacgg
      601 gggcccgatc ttgactccag agggcttcga accaccactg atcgttcgtg ccgtcgtagc
      661 ggagcagaga atcgtagtag gagaggccga atgcaggcag tgcggttgga cgcggtggcg
      721 ggtcgggaag ttgctcgatg ttgccgccca gttgatagcc gagatatccg aaccagccgc
      781 ctccgatggc tccgggcacc tgtccgatca ccttgggcat cgttgtcatc gtcaggaaag
      841 ggtcgtctct cgccgaggcc ctgtgttgtg ggtcagatgc cacgagagcg ccgcctccgg
      901 cccactcacc cgccaggacg acgaggtctt cgtcccccct gaaggagcgc agcacgtctt
      961 cgcgtggaag gtcggtcggg atctgctcac agatgaggtg aacaggttct tgccaagcag
     1021 gcatccgaaa ccttccttag gtcgctgggg ggaacgaaac tttacctcgc ctcgccgtga
     1081 ccaccagttc tggactcgct ctcgtgcccg ccctcggccc tgctagtttt gatgaatctg
     1141 atggtagtgt aagaggcatg aagatgctca ccacggggcg agtggctgct tcgcttggcg
     1201 tcaccataaa caccgtcaag aattgggctc gagctgggaa gatccaggcg gttcgcctgc
     1261 cttctggcca ctttcgtatt ccagcaaccg aggtcgatcg cttgctgcag ggccttcgct
     1321 cggcggggct tcgggagtac gagcagtgga gtcgcaaaca accgtcgacc ccccccaacc
     1381 tcgatcaggt gcttacctgg aatagacagg ctcttgagat cgcaaggtcg cagggaaaac
     1441 tccctaatga ctccttagaa gaaaagggtg aacgcgttcg gcggatgcat cggatcctcg
     1501 ccgcgctcag cagatgaacg atctcgaggg tgcagtggtg gctatcgcca tgactctcaa
     1561 ggcgaacgcg gttccttaca tggtcatagg tggattcgcc aacctgctgt ggggcgaacc
     1621 gcgaacgacg agagacctag acgttaccgt tgacatcgat ctggcggatt ggaaagaggt
     1681 cgacaaactg tgccagcaga taggaaggtt cttggcctct gatccgcaag caatggcgga
     1741 gcgaacgaga gttgtgcctc tcatgaccgc aggaggagtt ccagttgacc tgatcatcgg
     1801 catgctgacc tttgaatcag aggcgatcgc gcgggcccgt caagttgagt tccaaggcgg
     1861 tctggtcagc gtctgcaccc cggaagatct ggtcgtgcac aagatcattt ccgagagacc
     1921 cagggatctc gatgatgtcg tggggatcct gcgccgccag ggtggcgcta tcgatgtccc
     1981 cggattagag gctctggttt cgcaactttc gaccaccatg gatgatgcga cgatcatcga
     2041 ccgattcgtc gaagccaaac gactcgctaa cctggctccc tagatgagga gttggggccg
     2101 ggcaaggcat ctggagtgcg atgtgacact atttctgcgt gtctactgag tctgggctcg
     2161 aaacggccac agttgtattc accgacctag aagggtcgac tgaccttcga acgcggctcg
     2221 gtgatgaatt ggcccacgag cggatacgca tccacgaaga gatcatccgc gaacagatct
     2281 ccaagttcga aggtttcgag gccaaggcac tcggcgatgg gttcatgatc gtcttcggct
     2341 ctgcccggca ggcgatcaac tgctcggttg ggatccagat agcgatcgag gaacacaacc
     2401 atagtgatcc ggcgcgagcc gtcaaggtgc gaatcggcat caatagcggc gaggtaacgc
     2461 gcgaggcgag cgacgtatac ggcgccgcgg tgaatgccgc cgctcgcatc tgcgcgaagg
     2521 ctcagggtgg tcagatcctc atcccacaag tcgtgaagga ccttgctgga ttcgtcccag
     2581 acctccggat cgtcgatcgc ggtctctact ggctcaaggg attcccgcag cgatggcggc
     2641 tccacgaggt catgtggagg ggccgaggcg atgaggccgt cgaacgcgcc gatgccccca
     2701 cgatccctcg tatcagaact gacgtccccg aactgccgcg cgccttggcc cccatcgtcg
     2761 gacgtgccag cgagctagcc gccatcgacg aggaactgga acgagtaaca acggggaccc
     2821 ttcgcgtgct cgcattcgaa ggcgaagcgg ggataggaaa gacccgactc ttggaagccg
     2881 ctgcagtggc cgcgaaaagc aaagatctcg cggtgctccg cgttggcgca gatgaggagc
     2941 tacgcggacc gttcttcatc tttcgggcac tattcgcaac tgcagaagcc gaagcggtcg
     3001 ccgaggaggc tgtcgcgctg gaggccctag atcgagccaa ggatgcactt gccggacgtg
     3061 atgcggggca ccaggcgggc ttatctcctc aggagcagat gcttcatgtt ttcgacgagg
     3121 tcgcttcggc catacgcgcg ctggcttcgg gttcgccgct agcgctgctg ctcgatgatg
     3181 tgcagtgggc ggacgaagat agtctgcgac tccttcgcta catagcccgc actatcgccg
     3241 cctcgccgat cctggtgttg atgaccgtga gggagcaggt tgagagtccg accggaggca
     3301 ccgcgcatct gcttgcagat ctagaccgta tgcatctcgc cagacgtgtc cggctcccgc
     3361 gcttctcgaa gaagcagacc gcagaactcc tacggaatct gttgggatca ccggtggccg
     3421 aacagaccct agacatgctt catggacgag ccgagggcgt ccccttcttc gtagaggagt
     3481 tcgctcgcac ctatcgggag tctaaggcgc ttcagcaggt gggggagacc tgggtgatga
     3541 cgcagcttac cggaccatct gttccagcct cggtgcagtc gctcatcgaa cgca
//
LOCUS       DATDOZ010000046         4842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3184441, whole genome shotgun sequence.
ACCESSION   DATDOZ010000046 DATDOZ010000000
VERSION     DATDOZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184441"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VI541_00865"
     CDS             <1..450
                     /locus_tag="VI541_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF69487.1"
                     /translation="RWQGRRVVAALMDHPDLVRAIIVDDNEDIRLLLKMELERDGRFE
                     VLGEAADGLQGIMLAERTQPDVIILDLAMPVMDGLQALPELKRVCPAAKIVVLSAFSA
                     TMVAEEALACGADTYVEKGVAIKELADIIAGIVSGPMWTPSGSQLML"
     gene            514..1992
                     /locus_tag="VI541_00870"
     CDS             514..1992
                     /locus_tag="VI541_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF69488.1"
                     /translation="MSHSLLPEVEKSLQDPQKLFISGGFTDAASGRTFATVDPATGEE
                     ITSVAEAGRADVDVAVKAARTSVDSQVWSGMRPAQRARILWDIADRIEARKDELAQIE
                     TLDQGKPFNMAKAEMDLVASTFRYFAGWVDKYHGETDAGDGSMLVYTLREPVGVTVGI
                     TPWNYPLVMAAYKVAPALAFGNAMILKPAEQTPLTALRLGEIAAEAGVPEGIFQVLTG
                     DGSTGAALVEHPGVDKVAFTGSTEVGRAIMANAAKTLKRVTLELGGKSPNIVFADADM
                     KRASSTAMYGVFLNSGQTCTAATRLLVERSIHDEFVESVASASSKMKLGHGLDPQTRM
                     GPLVSKEQLDRVTGYIEVGTQDGATLAHGGGRPDGDLASGFFVEPTIFTGVKPGMRIA
                     REEIFGPVLAVIPVDDVEEAIRIGNDSEYGLAAAVWTNDLRKAHRVAAGLKAGTVWIN
                     TYGLYDPSVSFGGYKQSGFGRELGPHALEAYTQIKSVWLNLA"
     gene            complement(2015..2995)
                     /locus_tag="VI541_00875"
     CDS             complement(2015..2995)
                     /locus_tag="VI541_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLF69489.1"
                     /translation="MTKDDQPRSASDPLIWSALAIVYVVWGSTYLAIRVLAIEHAPAF
                     ISAGVRFVIAGAVMLLVGRLRTRRAPERITPHQLRSSVIVGGALLLGGNGMVVFAEQR
                     VDSGVAALMVASMPLWFALFLWLFHGERLPRPALVGLALGFGGIALLSWPSGTSTIAT
                     SGIVALLIAPMAWSAGSIYARRAPLPRDPFLAAGLETVAGGILLLAAAAATGEFRNLD
                     PHLTTRAWLSLSFLIVAGSLVAFTAYMWLVRNAPTSLVSTYAYVNPVVAVLLGSLLLQ
                     EPIGVRTVTSAAIIIAGVAMMVSTQARRQSDVVELSSPADAPVPVGIDHK"
     gene            complement(3035..>4842)
                     /locus_tag="VI541_00880"
     CDS             complement(3035..>4842)
                     /locus_tag="VI541_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HLF69490.1"
                     /translation="DLQKPIAVDGGHDNLHHSRSRLLTLPSTWATPTPEDAGGNDLFC
                     ADQRHLADGRVIAVGGTEWVTEQGAAVDDGNGGSFGPIELLGSKNTRIFDPATNQWAT
                     STAWDMNYRRWYPTLVTLPSGKLFVAGGVEKLIYNDAGTNVHNTETLDPNALSTGWVD
                     NGESGATSLPLFARLHLLPNGKVFYDATGQMWGPAGQGYDEVLWNMMKWYDPSTNSWT
                     DKGLAEFGARSGAFSVMLPLKPDNAGNYSKAKILIAGGTIGTSPSTYIANSISEIVSI
                     DGDSVTRKRTGDLNNRRWYSSGVVLPSGEVLALSGADKDEVIDPGSEFPVRQAEMYNP
                     TTKHWSRLASGSRDRTYHNSAVLLADGSVLVGGHSPIVSQYGNTHANYFGPPFANNFK
                     DPSFEILKPPYLFRGPRPTITAAPSNIGLGDDFEITTPDAGSITSVVLSRLPAVTHIT
                     DADQRTVELKIISQGSGTVKVKAPASAAVLPNGPYYLFLIRNNPAGIDHPAGPTPSIA
                     AVVNATGTSPSAGSGSGPSGLDLDSLTGSATPPTSPKQAASKVTNIFAADSSTGTPPG
                     SHRPRPGALLLATVAIATAFGAGDMRFRRRRTAKI"
BASE COUNT          898 a         1406 c         1626 g          912 t
ORIGIN      
        1 agatggcaag gacgaagggt agtggctgct ctcatggatc acccagatct cgtcagggcg
       61 atcatcgtcg atgacaacga ggacatcaga ctcctcctga agatggagct tgagcgcgac
      121 ggacggttcg aggtgctggg agaggctgct gatggccttc aggggatcat gcttgcagag
      181 cgcactcaac cggacgtgat catcctcgat ctcgcgatgc ccgtcatgga cggactgcag
      241 gcccttccgg aactgaagag ggtctgtccc gcggccaaga tcgtcgtact ctcggccttc
      301 agcgcgacca tggtggccga ggaagcactg gcctgcgggg ctgacactta cgtcgagaag
      361 ggtgtggcca tcaaggagct ggccgatatc atcgcaggga tcgtctctgg tccgatgtgg
      421 accccctccg gatcgcaact gatgctctga gccgggttcg tgcatggcgg aataggattc
      481 tccgaacccc taactatcga ggagagtccg tcgatgagtc attccctgct gcccgaagta
      541 gagaagtcac tgcaagaccc ccagaagctc ttcatctccg gcgggttcac cgatgcggct
      601 tccggacgga cctttgccac cgtagatccg gccacaggtg aggagatcac gtcggtagcc
      661 gaggccgggc gagccgatgt cgatgtcgcg gtgaaggccg ctcggactag tgtcgactcg
      721 caagtgtggt cgggtatgcg tcccgctcag agggcacgga tcctgtggga catcgccgac
      781 cgtatcgagg cgcgcaagga tgagctggcc cagatcgaga cactcgacca gggcaagccc
      841 ttcaacatgg ctaaggcgga gatggatctg gtcgcctcga cattccgata cttcgcaggt
      901 tgggtcgaca aataccatgg cgagaccgat gcgggtgacg gctcgatgct ggtctacaca
      961 ctgcgcgagc ctgtcggcgt gaccgtcggc atcaccccgt ggaactaccc cttggtcatg
     1021 gctgcataca aggtggcacc cgctctcgcc ttcggcaacg ccatgatcct caagccagcc
     1081 gaacagaccc cgctgaccgc gctgcgtctc ggcgagatcg ctgcagaggc cggggtcccc
     1141 gagggcatct ttcaggtgct gacgggtgat ggttcgacgg gggccgcgct tgtggagcac
     1201 cccggtgtcg acaaggtggc gttcaccggc tcgaccgagg tgggccgagc gatcatggcg
     1261 aacgccgcaa agaccctcaa gcgagtgacc ctcgagttgg gcggcaagtc ccccaacatc
     1321 gtcttcgcag atgcggatat gaagcgtgcg tcgtcaactg ccatgtacgg ggtgttcctg
     1381 aactctggtc agacctgcac cgcggcaacg cggctcctcg tcgagcggtc gatacacgat
     1441 gagttcgtcg agtcggtcgc atcggcgtcg tcgaagatga agttaggcca tggcctcgac
     1501 ccgcagacgc gtatgggtcc tctcgtttcg aaggagcaac tcgatcgcgt caccgggtac
     1561 atcgaggtcg gaacacaaga tggggcgact ctcgcgcatg gcggtggacg acccgatggg
     1621 gacctggcat ccgggttctt cgttgagccg acgatcttca ccggtgtgaa gccggggatg
     1681 aggatcgctc gcgaggagat attcgggccg gtgcttgctg tgatcccagt cgatgatgtc
     1741 gaagaggcga tacgcatcgg taacgacagc gaatacggac tggcggcagc ggtttggacg
     1801 aatgatctgc gcaaggccca tcgcgtggct gcgggtctca aggcggggac cgtctggatc
     1861 aacacatacg gtctttacga tccatcggtg tctttcggtg gctacaaaca gtccgggttc
     1921 ggtcgagagc tcggccccca tgcgctcgag gcttacacgc agatcaagtc ggtctggcta
     1981 aacctggcct gaccctgacc tagatgtcgg gggcctactt gtggtcgatc cccaccggta
     2041 ctggcgcgtc ggcggggctg gagagttcga cgacatctga ttgtcttctc gcttgggtcg
     2101 agaccatcat ggcaacgccc gcgatgatga tcgcagcgga tgtgaccgtt cggaccccta
     2161 tcggttcttg aaggagcagc gaccctagaa gcaccgcaac gacggggttc acatatgcat
     2221 aggtgctaac caatgacgtg ggggcgttgc gcaccagcca catgtaagcg gtgaacgcta
     2281 ccaacgagcc cgcaacgatg agaaagctaa gagagagcca ggcacgcgtc gtgaggtgcg
     2341 ggtcgaggtt gcggaactcg ccggtggcgg cagccgcggc aagcagcagg atccctccag
     2401 ccacggtttc gagtcccgcg gccaagaacg ggtcgcgggg taggggggcc cgtcgcgcgt
     2461 agatcgagcc cgccgaccat gccatcggcg cgatgagaag ggccacgatc ccagaagtcg
     2521 ctatggtgct ggttccgctc ggccatgaga gaagcgctat gcccccgaac ccgagggcca
     2581 ggccaaccag ggccggccgt ggcaggcgct ctccgtggaa taaccaaagg aagagagcga
     2641 accacaacgg catggatgcc accatgaggg ccgccacgcc ggagtccacg cgctgttcgg
     2701 cgaagacgac catgccgttg ccgccaagaa ggagtgcgcc gccgactatg acggacgaac
     2761 gcagttgatg aggggtgatc ctctcgggag ccctgcgtgt tcgcaacctg ccaacgagca
     2821 acatcaccgc acctgcgatg acgaaacgca cgccggcact gatgaatgcc ggagcatgtt
     2881 cgatcgcaag gactctgatg gcgaggtatg tagagcccca cacgacatag acgatggcca
     2941 gcgcggacca gatgagagga tccgatgcgg atcgcggttg atcgtccttc gtcatggatg
     3001 gagtttagtg ctgcccgaga tcgcgggccc cgattcagat cttggcagtg cgccttctgc
     3061 ggaaccgcat gtctccggcg ccgaatgccg tcgcgatcgc gacggttgcg agaagaagcg
     3121 ctccgggtct cgggcggtgc gacccgggcg gggttcccgt ggatgagtcc gccgcgaaga
     3181 tgttggtaac cttcgatgcc gcttgcttcg gcgaggttgg cggcgtcgct gaccccgtga
     3241 ggctgtcgag gtccagaccg cttggaccgg aacccgatcc ggcgctcggt gacgtgccgg
     3301 ttgcattcac aacggcagcg atggaggggg tcggtcccgc cgggtggtcg atccccgcgg
     3361 ggttgttgcg gatcaggaag aggtagtagg gcccattggg aaggactgcg gcgctggcgg
     3421 gggccttgac tttcactgtc cccgaaccct ggctgatgat cttgagttcc acggttcgct
     3481 ggtccgcatc ggtgatgtga gtcacagcag gcaggcgcga gagcactacc gaagtgatcg
     3541 aaccggcatc gggggtcgtg atctcgaagt cgtcgccgag cccgatgttc gaaggcgccg
     3601 ccgtgatggt gggtcggggc cccctgaaga ggtatggggg cttgaggatc tcgaaggatg
     3661 ggtctttgaa gttgttcgcg aacggggggc cgaagtagtt agcgtgtgtg ttgccgtact
     3721 gcgagacgat gggggagtgg cccccgacca gaacactgcc atctgccaga aggaccgcgc
     3781 tgttgtgata ggtacgatcg cgactcccac tcgcaagccg ggaccaatgt ttggtggtgg
     3841 ggttgtacat ctccgcctgt ctcaccggga actctgagcc ggggtcgatg acctcgtcct
     3901 tgtcggcgcc gctcagggcg agcacctcgc cggatggaag cacgacaccg ctcgagtacc
     3961 agcgccggtt gttcaggtcg ccggttcgct tccgagtcac gctgtcgcca tcgatgctga
     4021 cgatctcgga tatcgagttg gcgatgtagg tgctgggcga ggttccgatg gttccaccgg
     4081 cgatcaggat cttcgccttg gaatagttgc ccgcgttatc gggcttaagt gggagcatca
     4141 cggagaatgc gccgcttcgg gcaccgaact cggcgagccc cttgtcggtc cagctgttcg
     4201 tggatgggtc gtaccacttc atcatgttcc acagcacttc gtcataaccc tgtcccgccg
     4261 ggccccacat ctgacccgtt gcgtcgtaga agaccttccc attggggagc agatggagtc
     4321 ttgcgaagag cggcagagag gtggctccgc tctcaccgtt gtcgacccaa cccgttgaca
     4381 gcgcgttggg gtcaagtgtc tcggtgttgt ggacgttcgt gccagcatcg ttgtagatca
     4441 gcttctcgac accacccgcg acgaatagct tgccgctcgg gagcgtaacg agggtcgggt
     4501 accaccgccg gtagttcatg tcccaggccg tggaggtggc ccactgattc gtggctgggt
     4561 cgaagatccg cgtgttcttg ctaccgagca gttcgatcgg gccgaatgac ccgccgttgc
     4621 catcgtcgac agcggccccc tgttcggtga cccactcggt gccgccgacc gcgatgacgc
     4681 gtccatcggc gaggtggcgc tggtcagcac agaagaggtc attgccccca gcgtcctcgg
     4741 gcgtcggcgt tgcccaggtg ctcggcagag taaggagtct gctgcgactg tggtgcaggt
     4801 tgtcgtgacc gccatcgact gcgatgggtt tctggaggtc ct
//
LOCUS       DATDOZ010000047         5481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3699839, whole genome shotgun sequence.
ACCESSION   DATDOZ010000047 DATDOZ010000000
VERSION     DATDOZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699839"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VI541_00885"
     CDS             complement(<1..324)
                     /locus_tag="VI541_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69491.1"
                     /translation="MRFKAVFFDAGETLLGPHPSFHALFGDVLREHGEPSSEEAIQSA
                     FERIAPTMTEVLDNLGTETWSTSPDVSRAFWARVYRAAFDHLEIDDGQGVLVSALYDR
                     FTQYSS"
     gene            complement(321..929)
                     /gene="coaE"
                     /locus_tag="VI541_00890"
     CDS             complement(321..929)
                     /gene="coaE"
                     /locus_tag="VI541_00890"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077947.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLF69492.1"
                     /translation="MLVGLTGGIASGKSTVSRMLAERGAVIIDADEIAHSILEPGGAA
                     YDDAILRFGREIVGSDGRIDRAKLGAIVFSDADARHDLEAITHPRIFSEILRRVAQGR
                     DEASVVVVDAALLVETLPDRGKALGLDALIVVSARPRDQVERLISDRQMTRTQAQERI
                     AAQLPVEKRLAAADIVLDNRGSLADLETSVGLLWDELTGDSA"
     gene            complement(943..2472)
                     /gene="rpsA"
                     /locus_tag="VI541_00895"
     CDS             complement(943..2472)
                     /gene="rpsA"
                     /locus_tag="VI541_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HLF69493.1"
                     /translation="MSQVTGTTPDDREVITENNIGTTQEELMAAIDATIKPFADGDLV
                     SGTIVKIDKEEVLVDIGYKTEGVIPARELSIRHDIDPSEIVSLGDSIEALVLQKEDKD
                     GRLILSKKRASYERAWGRIEELRRSGEVVTGTVIEVVKGGLIVDIGLRGFLPASLVDL
                     RRVRDLAPFIGKELECKIIELDKNRNNVVLSRRAYLEEAHSEQRKEFLDNLQPGERRR
                     GTVSSIVNFGAFVDLGGVDGLVHVSELSWRHVDHPNEVVQVGEEVEVEILEVNLERER
                     VSLSLKNTQEDPWQIFSRSHGMGDVISGKVTKLVPYGAFLEVAAGIEGLIHISELSDA
                     HVELPEQVISLGQELEVRIIDIDLERRRISLSLKQASETSPMLMYSTSTAAPAEAEAV
                     AEAHDFAPEPGNEMVSEGAPALEVEAPAVAEVIEELAPVAEAAPIAEAAPVIAETVPE
                     IQTPAEPEAPAVPEPAPVVAEVVEVKPEPELEGELLERQEASSEDSLESIVEEMKRDR
                     S"
     gene            complement(2619..5276)
                     /gene="polA"
                     /locus_tag="VI541_00900"
     CDS             complement(2619..5276)
                     /gene="polA"
                     /locus_tag="VI541_00900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLF69494.1"
                     /translation="MTAPNPEPVLLFDGHSLAYRAFYALPSDLRTSSGQITNAVYGFT
                     SMLIKAIQDLKTQHVTVAFDSGPPLERLAIRPEYKAQRVSAPDEFRQQMGLIHEVLDV
                     LKVPVFEIPNVEADDILSVIATSVADSGGDAVIVTADRDYFQIVRPGIRLMMNRRGIS
                     DTVVYDTAAVIERFGFPPAQYLDYAALRGDPSDNIEGVPGVGEKTAARLIIKYGGLDE
                     LFENIDVQTPKLKENLLEARDRLFENRDFFRFRGREHLEAQGGTLDISVGGLAMGEWD
                     FDEIRKLFDALEFRVLFDRIAAGRPEAAVAGFEAEVTDVAGPSIDVVLARPHEGPLCV
                     VITAPGSREPATAVAVASVGGAEVLHPDPSQWSAVGEWLSARGIITHGCKEAVQALAA
                     RGLATPVVEMDTEIAAYLLEPARGSYGLDELARTYLEKELRLGAASEDEGQQSLSLEE
                     PGAEPSGAGIEVLAVAQVAAILTRELAQRGLLELLRDLEIPLALVLAKVEDAGVRIDV
                     DYLKSLSDEAGDELSDIEAKIYEMAGEPFNIGSPPQLRSILYDKLGLKPGKKTKTGYS
                     TDASVLESLREEHQIVDAILRHREISKLKSTYLDALPPLVDPKTGRVHCHFNQTVAAT
                     GRLSSDSPNLQNIPIRTEEGKRIRKAFIPDEGHLLIVADYSQIELRILAHLSKDPNLV
                     DAFRRGEDIHKLSISKALGIGVTEVTSELRSIGKMVSYGVTYGMGAFGLSQRLKIPMD
                     QARTYIDGFFGLYPGVRQYLDAVVEQAAVDGFTTTMLGRRRYVPELRSRNPRVRSLGE
                     RQALNAPIQGSAADILKLAMVKTDEALRGFDARMVLTVHDELVFEAPTKEVDRVAVAV
                     KEAMESVVDLDVPLSVDVASGPNWAEAKA"
BASE COUNT         1013 a         1706 c         1652 g         1110 t
ORIGIN      
        1 tgagctgtac tgggtgaagc ggtcgtagag ggcggacacg aggacccctt gaccatcatc
       61 tatctcaaga tggtcgaagg ctgcccgata gacccttgcc cagaatgcgc gcgagacatc
      121 gggcgaagtc gaccaagttt cggtccccag gttgtcgagg acttcagtca tagtcggagc
      181 gatcctctcg aatgccgact gaatggcttc ttcgctcgac ggctcgccgt gttcacggag
      241 aacatcgccg aagagagcgt ggaacgacgg gtgggggccg agcagcgtct cgccagcatc
      301 gaagaagact gctttgaatc tcatgcgcta tcaccggtta gttcatccca gagaagaccg
      361 acggatgtct cgagatcggc aaggcttcct cggttgtcga ggacgatatc cgcggcagcc
      421 aagcgtttct cgacaggcag ctgcgctgcg atgcgctcct gagcttgggt ccgcgtcatc
      481 tgacggtcag agatcaatcg ctcaacctga tcgcgaggtc gagctgacac cacgatcagc
      541 gcatcaagac cgagtgcttt gcctctatcg ggaagggtct caaccaacag ggccgcatcg
      601 accaccacta cagacgcctc gtcgcgaccc tgggccactc gccggaggat ctcgctgaag
      661 atgcgcgggt gcgtgatcgc ctctaggtcg tggcgcgcat ctgcatctga aaacacgatg
      721 gcccccagct tcgctcgatc gatcctgcca tccgacccga cgatctccct accgaagcgc
      781 aggatggcgt cgtcgtaagc cgctccgccg ggctcgagga tcgagtgcgc gatctcgtcg
      841 gcatcgatga tcaccgcgcc gcgctcggct agcatcctgg acacggttga cttcccggat
      901 gctatccctc cggttagacc gaccagcatc atctatgcga cgttagctac ggtcgcgctt
      961 catctcttcg acgatcgact ccaacgagtc ttcggagctg gcctcttggc gctccagaag
     1021 ttctccttcg agctcgggtt cgggcttgac ctccacgacc tccgccacga cgggagcagg
     1081 ctcgggtact gccggagcct cgggctcggc gggagtctgt atctcgggaa cagtctccgc
     1141 gatgaccggt gcggcctccg ctatcggcgc ggcttcggca accggtgcca actcctcgat
     1201 tacctcggcc acggcaggtg cttcgacctc tagagccggg gccccctcag agaccatctc
     1261 gttgccgggt tcgggagcga agtcgtgggc ctcggccaca gcctcggcct cggccggtgc
     1321 ggccgtgctc gtcgagtaca tgagcatcgg cgaggtttcg gatgcctgct tgagcgacag
     1381 actgatccgg cggcgctcga ggtcgatgtc gatgattcgg acttcgagtt cctgccccag
     1441 tgagatcacc tgttccggca gctcgacatg cgcgtcggag agctctgaga tatggatcaa
     1501 gccctcgatg cccgcggcga cctcgaggaa ggcaccgtat ggcacgagct tggtcacctt
     1561 gcctgagatc acatcgccca tgccgtggga gcgagagaag atctgccagg ggtcctcttg
     1621 agtgttcttc aacgagaggg aaactcgttc tcgctcgaga ttgacctcca ggatctcgac
     1681 ctcgacctct tcgcctacct gaacgacctc gttcggatga tcgacatggc gccaagagag
     1741 ttccgagaca tgcacgagac cgtccacgcc cccaaggtcg acgaaggcgc cgaagttgac
     1801 gattgatgag acggttccac gccttctctc gccgggctga agattgtcga ggaactcctt
     1861 gcgctgctcc gaatgagcct cttcgaggta ggcccttcgg gacaggacca cattgttacg
     1921 gttcttgtcg agttcgatga tcttgcactc gagttccttg ccgatgaaag gcgccagatc
     1981 ccggacccta cgaagatcta caagtgaggc tggcaggaat ccgcgcagac cgatgtccac
     2041 gatcagtcca cccttgacga cttcgatcac cgtgcccgtc acaacctcgc cggatcggcg
     2101 cagctcctcg atcctgcccc aggcgcgttc gtaggacgct cgcttcttcg agaggatgag
     2161 acggccgtcc ttgtcttcct tctgcaggac gagggcctcg atcgaatcac cgagactcac
     2221 gatctcggat gggtcgatgt catggcggat gctcagctcg cgagctggga tgacaccttc
     2281 ggtcttgtag ccgatatcaa cgagtacttc ttccttgtcg atcttgacga tagttccgct
     2341 cacaagatcg ccatcggcga aaggcttgat cgtcgcgtcg atagcggcca tcagctcctc
     2401 ttgggtagtg cctatgttgt tctccgtgat gacctcgcgg tcgtcaggag tagttccagt
     2461 tacttgggac atctggtgct ccttcgtgcc ctctaggggc ggggtactcc tcggcgggtg
     2521 accgtcacga gggcccaaga atcttagcat cccaactcgg cccccggagc ctgaaaggac
     2581 ggatctggct gaaaatcagg cgtttcctca ggggtttctc aggccttggc ttctgcccag
     2641 ttgggccccg acgccacatc gacgctcaac gggacatcca gatcgaccac cgactccatc
     2701 gcctccttga ccgccacggc gactcggtcg acctccttcg tgggggcctc gaataccagt
     2761 tcgtcatgca ccgtgagcac catcctggcg tcgaacccgc gcagcgcctc atcggttttc
     2821 accatcgcca gctttaggat gtcggcggca gatccttgga tcggagcgtt gagggcctgc
     2881 ctctcgccga ggcttcgcac gcgcggattc ctggagcgca attcgggcac gtatcgccgg
     2941 cggcccagca tcgtcgtagt gaatccgtcg accgcggcct gctcgaccac cgcgtcgaga
     3001 tactggcgca cccccgggta gaggccaaag aaaccatcta tatatgtccg agcctggtcc
     3061 atcgggatct tgagcctctg agagagtccg aaggctccca tcccgtaggt cacgccgtac
     3121 gagaccatct tgccgatgct gcgcaactcc gaggtgactt cggtgacacc tatccccagg
     3181 gcctttgaga tcgagagctt gtggatgtcc tcgccgcgac ggaacgcgtc tacgaggttg
     3241 gggtctttcg aaagatgagc gaggatccgc aactcgatct gcgagtagtc ggcgacgatg
     3301 agcagatgtc cttcgtcggg gatgaacgcc ttacgtatcc gctttccttc ctctgtacgg
     3361 atggggatgt tctgcaggtt ggggctatcc gagctcaatc gacccgttgc cgcgacggtc
     3421 tgattgaagt ggcagtggac gcggccggtc ttcggatcga cgagtggcgg caaggcatcg
     3481 agataggttg atttcaactt cgagatctct cgatgacgaa ggatggcatc gactatctgg
     3541 tgctcctcgc gcagagactc gaggacgctc gcgtcggtgg agtaacccgt cttcgtcttc
     3601 ttgcccggct tcaacccgag cttgtcgtag aggatgcttc gcagctgagg aggcgacccg
     3661 atgttgaagg gctcccccgc catctcgtag atcttcgctt cgatgtccga gagttcatcc
     3721 ccagcctcat ccgagaggct cttgaggtaa tcgacgtcga tgcgaacccc ggcatcctcg
     3781 accttggcga gcacgagagc gagaggtatc tcgagatctc tcagaagttc gagaaggccg
     3841 cgctgggcaa gctctcgagt caggatcgct gcaacctgag caacggccag gacctcgatc
     3901 cccgcccccg atggttcggc ccccggctcc tcgagtgaga gcgattgctg accctcatcc
     3961 tcggacgctg cccccagcct taactctttc tccaggtagg tacgagcgag ttcgtcgagc
     4021 ccatacgatc cccgtgcagg ctcgagcagg taagcggcga tctccgtgtc catctctacg
     4081 acgggggtcg caagccctcg tgctgctagg gcctgcaccg cctccttgca cccatgagtg
     4141 atgatcccgc gagccgataa ccactctcca accgctgacc actgggacgg gtcagggtgc
     4201 agaacctcgg cccccccaac actcgctacc gctacggccg tcgctggctc gcgcgagccg
     4261 ggggccgtta tgacaacgca gaggggccct tcgtgagggc gcgcgagcac cacatcgata
     4321 gaaggaccag cgacatctgt gacctctgcc tcgaaacctg cgacagcagc ctccggccgt
     4381 cctgccgcga tgcggtcgaa tagcactcga aactcgagcg cgtcgaagag tttccggatc
     4441 tcgtcgaagt cccactcccc catggcgaga cccccgaccg atatgtccaa cgtcccgccc
     4501 tgagcttcga ggtgttcgcg cccgcggaac ctgaagaagt cgcggttctc gaatagccgg
     4561 tcgcgggctt cgagcaggtt ctccttgagt ttgggggtct gaacatcgat gttctcgaaa
     4621 agttcgtcga ggcccccgta cttgatgatc agcctcgccg ccgtcttctc gcccacgccc
     4681 ggaacgccct cgatgttgtc ggagggatcg cctcgaaggg ccgcgtagtc gaggtactgc
     4741 gctggtggaa acccgaaccg ctcgatgacc gctgcggtgt cgtagacgac cgtgtcggat
     4801 atccccctgc ggttcatcat cagtcttatt ccgggccgga cgatctggaa gtagtcgcgg
     4861 tcggccgtga cgatcaccgc atctccgccc gaatcggcga cgctggttgc gatcaccgag
     4921 aggatgtcgt cggcctcgac gtttgggatc tcgaagacgg gaaccttcag gacatcgagg
     4981 acctcgtgta tcaaacccat ctgctgtctg aactcgtcgg gagcagagac gcgttgtgcc
     5041 ttgtactcgg gccggatcgc gagacgctcg agcggggggc ccgaatcgaa cgcgacggtt
     5101 acgtgctgcg tcttgaggtc ctggatcgct ttgatgagca tgctcgtgaa gccatagacg
     5161 gcgttcgtga tctgaccgct cgaggtccgc aggtcggatg ggagcgcgta gaaggcgcgg
     5221 taagccaggg agtgtccatc gaacaggagt accggttcag ggttcggggc agtcactgac
     5281 ggggaatata gtaggccagc catgatcaag caggtcgctg tggcgactcc taagctccgg
     5341 cgggccgagg gaagcactgc tgcagaaggt ctgagggttt gacaccaggg ccctgggccc
     5401 ccacaatcct gatagagcaa aggcgctccc gatccggggg cgtcttttct ttttctaacc
     5461 gagtctccgt acaggctgac a
//
LOCUS       DATDOZ010000048         3727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2912353, whole genome shotgun sequence.
ACCESSION   DATDOZ010000048 DATDOZ010000000
VERSION     DATDOZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912353"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VI541_00905"
     CDS             complement(<1..673)
                     /locus_tag="VI541_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HLF69495.1"
                     /translation="MSGDAVVLDVGGLEVQITNPDKVFFPELGLTKMDLVSYYLAVAP
                     GVLVGCRDRPTSLKRFPNGIHEEFFFQKRVPAHRPEWIRTAWVTFPSGRTAEFLTILD
                     AAHLAWAINLGCIDINPWPVRSSDLDHPDELRVDLDPTPDIPWRHVREVALLVREVLN
                     EFGLTGFPKTSGSRGIHINVRIEPSWEFTEVRRAALALAREVERRSDLATTAWWKEQR
                     VGVFLD"
     gene            710..1198
                     /gene="purE"
                     /locus_tag="VI541_00910"
     CDS             710..1198
                     /gene="purE"
                     /locus_tag="VI541_00910"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HLF69496.1"
                     /translation="MEPLVGIIMGSHSDWEPMSAAAETLDRLGVSYETRVLSAHRTPD
                     ALLAYVQTAAERGLKVIIAGAGGAAHLPGVTAAKTDLPVLGVPIESKALKGMDSLLSI
                     VQMPGGVPVGTLGIGKSGATNAALLAARILALADPDIAKSVVAHREEQTRKALERTDP
                     RE"
     gene            1226..2764
                     /locus_tag="VI541_00915"
     CDS             1226..2764
                     /locus_tag="VI541_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLF69497.1"
                     /translation="MIGSGPNGLVAACILAMKGWKVTVVERSDRAGGAVRSDELTAPG
                     FIHDTYSAFYGMLHASPVLKELGLDTRIKWADFEIPVAAVTTRERAALCFRDSASTAS
                     HLGELASGDGDAWQDLYGWWRRSGRHFLSATLASIPSLRPTVRFAASTGLRHALPTAG
                     MLLSTMRSLAEDRFTSQEARSLLACGASHADVSIDATGSVPPALILAMVAQEKGMPIP
                     VGGASRIPDELVTILRSNGGSLVLDSAVSRVIAEHGRAVGVETSDGRYFRARRAVLAD
                     VGVKQLFQHLVTDSFLPRQFLDGVRRFRHGTGFFKMDMALSGPVPWLAGGLAKTGVIH
                     LTGDLHQMSMSMNEVAAGALPTRPLLIVGQQTVADPTRAPAGKHTLWVETSVPSTPMQ
                     GGTRLDWRELARPFGDTVLSLLEDHAPGVSSLIEGLSIKTPQDLEAANPNLVGGDPGG
                     GSMSLDQQLIFRPVPGWFRYRTPIKGLYLCSASAHPGGGVHGMVGRNCAERVLRDRLS
                     PRRR"
     gene            complement(2761..>3727)
                     /locus_tag="VI541_00920"
     CDS             complement(2761..>3727)
                     /locus_tag="VI541_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HLF69498.1"
                     /translation="VVPDLGAFEIVRPMGVQQSNSSLVYDERVVLKLLRQITDPSRDL
                     EMTKALWSAGFRQVAEPIGVWARQGRDLAMAQRYLGGGVEAWALARTSLRDLFGSGNE
                     PEIAGGDFAGDCRRIGETTAQMHRVLAQAFGSEPAEPRSWVDLMNSSLERVSDPRLDV
                     DAARGLFDSLADLADAGSSMRIHGDYHLGQLVETDDGWFVLDFEGEPARPAAERRAPS
                     SPLRDVAGMLRSLDYAARVVEAEHENPHGSQAESWIQRNREAFLDGYTQAVGGLDLLP
                     SDDTSFKMILRAFELDKAIYEVGYERAHRPDWVEIPLRAVESMLA"
BASE COUNT          707 a         1190 c         1131 g          699 t
ORIGIN      
        1 agtcgaggaa gacccctacg cgctgttcct tccaccacgc ggtcgtcgca aggtcgctac
       61 gacgctccac ctcgcgagca agtgcgagag ccgcccttct cacctcggtg aactcccatg
      121 atggctcgat gcgaacgttg atgtgtatcc cccttgagcc agacgtcttt gggaatcccg
      181 tcaacccgaa ctcgttcaag acctcccgaa ccaacagagc cacttcgcgc acatgtcgcc
      241 aggggatgtc aggggtcgga tcgaggtcaa ccctcaattc atcgggatga tcgagatccg
      301 aactgcgcac cggccagggg ttgatatcta tacaacccag attgatcgcc cacgcgaggt
      361 gggccgcatc gaggatggtg agaaactcgg cagtgcggcc gctcggaaat gtgacccaag
      421 ccgtgcggat ccactctggt ctgtgggcgg gaacacgctt ctggaagaag aactcctcgt
      481 gtatcccgtt ggggaatctc ttgagagatg tcggtcggtc gcggcacccc acgagcaccc
      541 cgggtgcaac ggcgaggtag tagctcacga ggtccatctt cgtgagccca agttcaggga
      601 agaacacttt gtctgggttg gtgatctgga cctcaaggcc cccgacatcc aggaccaccg
      661 catcgccact caccagtaga tattagttcg tgggctacaa tcgcccacga tggaaccgtt
      721 ggtcggaatc atcatgggtt cgcactccga ttgggagccc atgagtgcag cggctgagac
      781 cctggaccgt ctgggcgtct cttacgagac gcgcgtactg tccgcacatc gaacaccgga
      841 cgcactcctc gcttacgttc agaccgctgc cgagcgcggg ctgaaggtga tcatcgccgg
      901 ggcaggcgga gcggctcatc tgccgggcgt gaccgcggcc aagaccgatc taccggttct
      961 cggagtcccg atcgagtcga aggccctcaa aggcatggac tcgctccttt cgatcgttca
     1021 gatgccggga ggggtccccg tcggaactct tgggatcggc aagtcggggg ccacgaatgc
     1081 agcccttctt gcagccagga tcctcgcgtt ggcagaccca gacatcgcca agagcgttgt
     1141 tgcccaccgc gaagaacaga cccgaaaggc tctcgagcgc acggaccccc gggagtgatc
     1201 cacgcctgac atagccgacg cgatcgtgat cgggtcgggc ccgaacggac tcgtcgcggc
     1261 ctgcatcctg gcgatgaagg ggtggaaggt cactgttgtc gagaggtccg atcgtgcggg
     1321 tggtgcggtc cgcagcgacg agttgactgc gcctgggttc atccacgaca cctactctgc
     1381 tttctacggg atgctccacg cctcgcctgt actgaaggag ttgggtctcg acacgcgcat
     1441 caagtgggca gacttcgaga tcccggtcgc cgcggtcacg acgcgagaga gggccgctct
     1501 ctgcttccgc gactcggcct ccaccgcatc gcacctcggt gaactggctt ctggcgatgg
     1561 cgatgcttgg caggacctct acggctggtg gcgccgtagc ggcaggcact tcctctcggc
     1621 gacattggcc agcatcccat cgctccgacc aacggtccgc ttcgccgcct cgaccggtct
     1681 tcggcacgcg cttcccaccg cgggcatgct gctgtcgacg atgcggtctc tcgccgaaga
     1741 tcggttcacg tcgcaggagg cacgttcact tcttgcatgt ggcgcatctc acgctgacgt
     1801 cagcatcgac gctaccggaa gtgtccctcc cgcattgatc ctcgcgatgg tcgcgcagga
     1861 gaaggggatg ccgatcccgg taggtggggc gtcacgcatc ccagatgaac tggtcaccat
     1921 cctgagatcg aacggcggga gcctggttct cgactctgcg gtatcgcgtg tcatcgccga
     1981 gcatggccgc gctgtcggtg tcgaaacatc cgatggacga tacttccgag cgcgccgcgc
     2041 cgtgttggct gatgtcggcg tgaaacagtt gtttcagcac ctagtcacgg actcattcct
     2101 tccgcggcag ttcttggacg gcgtgaggcg cttcagacat ggcacgggct tcttcaagat
     2161 ggacatggca ctttccgggc ctgtcccctg gctcgcgggg ggcctggcca aaactggcgt
     2221 catccacttg accggagacc ttcaccagat gtcgatgtcg atgaatgaag tagcagcagg
     2281 agcgcttcca accagaccgc ttctcatcgt cggacagcag acggtcgccg acccaaccag
     2341 ggcacccgcg gggaaacaca ctctctgggt cgagacctcg gttccatcca cgccgatgca
     2401 gggcggaact cgtcttgatt ggagagaact cgcgcgccca ttcggcgaca ctgtcctatc
     2461 gctcctggag gatcatgccc ccggtgtttc gagcctcatc gaaggtctgt cgatcaagac
     2521 tccgcaggac ctcgaagcag ccaaccccaa cctcgtcgga ggcgacccgg gcggcggctc
     2581 gatgtcgctt gaccaacagt tgatattcag accggtcccc ggctggttca gatatcgaac
     2641 gcccatcaag gggctctacc tatgctcggc ctccgcgcac cctggtggcg gggtccacgg
     2701 gatggtgggc cgcaactgcg ctgagcgcgt acttcgcgat cggctgagtc cgcgccggcg
     2761 ctaggccagc atcgactcaa cggcgcgaag ggggatctcg acccagtccg gtcggtgggc
     2821 ccgctcgtat ccgacctcgt agatggcctt atctagttcg aatgcgcgaa ggatcatttt
     2881 gaaggaagtg tcatcggacg gcagaaggtc taggccccca accgcctggg tgtacccatc
     2941 caagaaagcc tcacgattgc gctggatcca ggactcggcc tggcttccat gcgggttctc
     3001 atgctccgct tcaaccaccc gggcggcgta gtcgagcgac ctgagcatcc ccgcgacgtc
     3061 tcgcaggggc gagctggggg cccggcgctc tgctgcgggc ctggctggtt ctccctcgaa
     3121 gtcgagcacg aaccaaccat catcggtttc aaccaactga ccgaggtgat agtcaccatg
     3181 tatgcgcatc gacgaacctg cgtcagcgag atcggccagt gaatcgaaca gccctctcgc
     3241 cgcatcgaca tctaagcgag ggtcggagac ccgttccaag gaagagttca tcaggtccac
     3301 ccaacttcgt ggttcggctg gctcagatcc gaaggcttgc gcgagaacgc gatgcatctg
     3361 ggcagtcgtc tcaccgatcc tgcggcagtc ccccgcgaag tcaccgccgg cgatctcggg
     3421 ttcgttgccg gaaccgaaga ggtctctcaa cgatgttcgg gcgagcgccc aagcctcgac
     3481 accaccacct agatatcgct gagccatggc caggtcgcga ccttggcgcg cccagacacc
     3541 gatcggctcg gcgacctgac ggaatccggc actccaaaga gcctttgtca tttcgagatc
     3601 acgactcgga tcggtgatct gcctcaagag tttcagaaca accctctcgt catagacgag
     3661 agaggagttg gactgctgca ctcccatggg gcgaacgatc tcgaaggccc ccaagtcggg
     3721 gacgacc
//
LOCUS       DATDOZ010000049         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2140536, whole genome shotgun sequence.
ACCESSION   DATDOZ010000049 DATDOZ010000000
VERSION     DATDOZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140536"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VI541_00925"
     CDS             <1..608
                     /locus_tag="VI541_00925"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLF69499.1"
                     /translation="HFSLLPRHRGAAPVQWALIEGDDETGVTIMRMDEGMDTGNIVTT
                     VREPILPGDTTGSLSKRLSIKGAPLLVDVIDRRDGDFRGVAQDGSQATYAPKLRSTDA
                     KLDWTLDADAIERKVRAFDPRPGAWGILDGKRLKFWEVAVGNDDPQIPPGSLVVEREV
                     MKISTGSNSIECRVVQPEGGSRMSAAEFLRGHRPSTPAKFS"
     gene            605..1270
                     /gene="rpe"
                     /locus_tag="VI541_00930"
     CDS             605..1270
                     /gene="rpe"
                     /locus_tag="VI541_00930"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLF69500.1"
                     /translation="MTPELAPSILSADFSRLAEQVASVEPYAGRIHVDVMDGHFVPNL
                     TMGPVVVASIRPHTRIPIEVHLMVTDPAMFIDSFAAAGADRIIFHVEVGEDPDSVIDK
                     IVGTRTGVGIALNPQTSFDLVEQYMRRIDLVTIMTVNPGFGGQSFMHEVIPKIEQARK
                     VSDRLGGKVQIEVDGGVDSSTIEAARSAGADVFVAGNAIFGAEDPVLAARSLARMLGL
                     EHA"
     gene            1263..2198
                     /locus_tag="VI541_00935"
     CDS             1263..2198
                     /locus_tag="VI541_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF69501.1"
                     /translation="MPDRVLIVDDDPDIVRFVQVNLELEGFEVSTAKDGEDALLKVES
                     DHPDLVLLDVMMPKMDGFEVCARLRADDRSRHLPIIMLTARALSSDKVSGLTAGADDY
                     IIKPFDPLELSARVKTTLRRAREMRDLSPLTGLSGNVVIVSELNSRIASARSFALMHV
                     DLDNFKAYNDHYGFARGDVALKETAGLLRACMEKHPGAEPSFLGHIGGDDFAIICDPT
                     ISVELAEEIVRSFDEFVPSLYDPEDLEAGYIVVTDRRSEPQKYPMLTISLGIANNLKR
                     EIHSHLEASAIANEMKHFSKMREGSSYSVDRRAGL"
     regulatory      2215..2343
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2437..>2975
                     /gene="ribD"
                     /locus_tag="VI541_00940"
     CDS             2437..>2975
                     /gene="ribD"
                     /locus_tag="VI541_00940"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HLF69502.1"
                     /translation="MSRALVLARRGKGKTNPNPMVGAVLVVRGRIVGEGFHKRAGDPH
                     AEINAIEAASGRCAGSTLYVTLEPCVHHGRTPPCVEAIVAAGVESVVVAMLDPDERVR
                     GKGVKALRKAGLQVEVGDGQHDAAILNRSYIMQRSQGRPFVTYKAAVSLDGRTAAVNG
                     SSRWISGDASRSDVHRLRAA"
BASE COUNT          608 a          851 c          919 g          597 t
ORIGIN      
        1 tgcacttctc actgcttccg cgccatcggg gagccgcacc ggtacagtgg gctctcatcg
       61 aaggcgacga cgagactggc gtcacgatca tgaggatgga cgagggcatg gataccggaa
      121 acatcgtcac aaccgtgcgg gaaccgatcc tgccaggaga cacgaccggc tccctctcga
      181 aacgtctctc gatcaagggg gctccgcttc tggtcgatgt tatcgatcgc cgggatgggg
      241 acttcagagg agttgctcag gatggatccc aagctaccta cgcaccgaaa ctcagatcga
      301 ccgacgcgaa gctggattgg accctcgacg ccgatgcgat cgaacgtaag gtccgcgcct
      361 tcgatccgcg accgggggcc tggggcatcc tggatgggaa gcgcctcaag ttctgggagg
      421 ttgcggtggg caacgatgat ccccaaattc cgcctgggtc gctcgtggtc gaacgagagg
      481 tcatgaagat ctccaccggc tcaaactcga tcgagtgtcg cgtggttcag cccgaaggtg
      541 ggtcgcggat gagcgcagca gagttcctgc gcggacaccg gccttcgacc ccggccaagt
      601 tctcatgacc cctgaactcg ctccgtcgat cctctcggcc gacttctcgc gactggccga
      661 gcaggtcgct tctgttgagc cctacgctgg ccgcatccat gtcgatgtga tggacggcca
      721 cttcgtgccg aacctgacga tgggccccgt cgtcgtcgcg tccatccgac cacatacccg
      781 tatccccatc gaggtgcacc tgatggtgac cgacccagcg atgttcatcg actcattcgc
      841 ggcggccggt gccgacagga tcatctttca cgtagaggtg ggtgaggacc ccgactcggt
      901 catcgacaag atcgtgggga ccaggacggg cgttggcatc gctctgaatc cgcagacgag
      961 tttcgacctc gtcgagcaat atatgcgcag gatcgatctc gttacgatca tgacggtcaa
     1021 ccccggtttc ggtgggcagt ccttcatgca tgaggtcatc cctaagatcg aacaggcacg
     1081 gaaggtgagc gaccgactcg gcggcaaggt ccagatcgaa gtggatggtg gtgtcgactc
     1141 atcaaccatc gaagcggccc gctcggctgg ggcagatgtc ttcgttgcag gcaacgccat
     1201 attcggcgct gaagacccgg tcctcgcagc gaggtctctc gcacggatgc tggggctcga
     1261 acatgcctga tcgcgtgctc atagttgacg acgacccaga catcgtacgt ttcgtccagg
     1321 tcaatctaga actcgaaggg ttcgaagtat cgacggctaa ggatggcgaa gatgctcttc
     1381 tcaaggtcga atcagatcac cccgatctgg ttctgctcga tgtgatgatg cccaagatgg
     1441 acgggttcga ggtttgtgct cgcttgcgag ctgatgatcg ctcgcgtcat ctaccgatca
     1501 tcatgttgac ggcaagggcc ctgtcctcag acaaggtgtc ggggctaact gccggcgctg
     1561 acgactacat catcaagccg ttcgatcctc tcgagttgtc ggcccgcgtg aagaccaccc
     1621 tgcgaagggc tcgcgagatg cgcgatctga gccccctgac cggtctttct ggcaatgtgg
     1681 ttatcgtctc ggaactcaat tctcgcatcg ccagcgcgag gtccttcgca ctgatgcatg
     1741 tcgatctcga caacttcaag gcctacaacg atcactatgg gttcgccagg ggagatgtcg
     1801 ccctgaagga gacggcagga ctcttgcgcg cgtgcatgga gaaacatccg ggggccgagc
     1861 catctttcct gggtcacata ggaggagatg attttgcgat catctgcgac ccgaccatca
     1921 gcgtggaact ggccgaagag atcgtccgga gcttcgatga attcgtaccc tccctatacg
     1981 atcccgaaga tctcgaagcg ggatacatcg tcgtgacgga tcggcgctct gaacctcaga
     2041 agtatccgat gctcaccatc tcactgggga tcgctaacaa tctgaagcgc gagatccact
     2101 ctcatctcga ggctagcgct atcgcaaatg agatgaagca cttctcgaag atgagagaag
     2161 gaagctccta ctccgtcgat aggcgtgcgg gcctataatc aacctaatcg aatagatagc
     2221 cttcggggca cggtgtaatc ccggaccggc agttacagcc tgcgacccac cgtcgttttg
     2281 gcgggggtgg acctggcgaa attccagggc cgacagtgaa agtctggatg ggagaaggct
     2341 caaagcacgc tcgtaccagg gcttgcttgg ttctgtcttg ccccgaagat cttgaaacga
     2401 cgggagagcg agatagaaac cgagtcatcg aagttcatga gcagggcact agtcctcgct
     2461 cggcgcggca agggtaagac gaatccgaac cccatggtcg gcgcggtgtt ggttgtccgc
     2521 ggccggatag tcggtgaggg gttccacaag cgagccggcg atcctcacgc tgagattaac
     2581 gccatcgagg ccgcctccgg ccgctgcgcg gggtcaacct tgtacgtgac attagagcca
     2641 tgcgttcatc atggaaggac tcccccctgt gtcgaggcga tcgtcgcggc aggggtcgag
     2701 agtgttgtgg tggcgatgct agatcccgat gagcgtgtcc gcggcaaggg cgtgaaggct
     2761 ctgcgcaagg ccgggctcca ggttgaggtc ggcgatggcc agcatgatgc cgcgatcttg
     2821 aatcgaagtt acatcatgca gagaagtcag ggtcgtccat tcgtgacgta caaggcagcg
     2881 gtctcgcttg acgggcgtac cgcggcggtg aacggttcgt ctcggtggat atcgggtgac
     2941 gcatccagat ctgacgttca taggctgcgc gccgc
//
LOCUS       DATDOZ010000050         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3436089, whole genome shotgun sequence.
ACCESSION   DATDOZ010000050 DATDOZ010000000
VERSION     DATDOZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3436089"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..460
                     /locus_tag="VI541_00945"
     CDS             119..460
                     /locus_tag="VI541_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HLF69503.1"
                     /translation="MSTLIGAVALREVEMSEKDDRSMLSRIAGPLLGIVGARYAMKGM
                     KGAWRFTFKSEPPGEDEERVGRRIAWVGISTAVVGMAAEFMRYIVGGRRTPKSSQDQA
                     KEQSEGGNSSG"
     gene            453..1745
                     /locus_tag="VI541_00950"
     CDS             453..1745
                     /locus_tag="VI541_00950"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLF69504.1"
                     /translation="MAEVIGVGLLGCGIVGSAVARILDAHVEEVSERVGARLEVRRVA
                     VKDLGKKRDTPLDPAIFTTKPSEVVSDPEVGIVVEVMGGIEPARTLMIEAMGRGKHVV
                     TANKELLSTHGKDLFDAAEHAGVDLYFEASVAGGIPIIRPLKESLAGERITRVMGIVN
                     GTTNYILTRMSEEALDFNSALADATRLGYSELDPTADIEGFDAAAKLAILSSIAFNAR
                     VVASDVYREGISSVTARDIAYGHQLGYEVKLLAIGEKVGGEVSVRVHPAMIPKHHPLA
                     SVRENLNAVFVEGEQVGELMFLGRGAGGPPTGTSVVGDLVEVARNMVSGGRGPGCTCY
                     RDDARIRSMDQTMSQHYILLEVFDRPGVLAEVARSFADHNVSIKSLWQEGYGDEASLV
                     MVTHSAVEHDVRELLRSLEKLESVDEVAASMRVQSSDE"
     gene            1746..>2571
                     /gene="thrC"
                     /locus_tag="VI541_00955"
     CDS             1746..>2571
                     /gene="thrC"
                     /locus_tag="VI541_00955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLF69505.1"
                     /translation="MPVWHGVIEEYRSRLPVGPDYPVITLGEGGTPLVHSKRLSHQTG
                     LEVFLKYEGANPTGSFKDRGMTVAVSMAVGSGAKAIACASTGNTSASAAAYAARAGIM
                     CAVLIPSGNVALGKLAQALVHGARTVEVRGNFDRALEITRKLEESYPVTLVNSINPYR
                     IEGQKTAAFEVCDALDGAPDYHLMPVGNAGNITSYWKGYREYLSDSIIDSTPRMFGFQ
                     AEGAAPIVRGYPIDSPKTIATAIRIGNPASWSQALEAAADSGGEIRAVRDKEILEAY"
BASE COUNT          512 a          731 c          829 g          499 t
ORIGIN      
        1 tccctcggta tcagtctcag cctcaagttg tacctttcta ttcacgatat gaaccgtctg
       61 agtggtggct gattgtctct agctgcacca ctcatcccct agactaccca agtcgaatgt
      121 gagtacgctc atcggagccg ttgcactgag agaggtggag atgtctgaga aggatgaccg
      181 ttcgatgctg tcacggatcg cgggtcccct cctgggcatc gtgggtgctc gttatgcgat
      241 gaagggcatg aagggcgcct ggcgattcac cttcaagagc gagcctcccg gtgaggacga
      301 ggagcgggtc ggccgtcgga tcgcctgggt ggggatcagc accgcggtcg tgggcatggc
      361 cgctgagttc atgagataca tcgttggggg ccgaagaaca cccaagtctt cgcaggatca
      421 ggcgaaggaa caatcggagg gtggaaactc gagtggctga ggtgatcgga gtcggtcttc
      481 tgggctgcgg gatcgtcggg tcggccgtgg cgcggatact cgacgcccat gtagaagagg
      541 tatccgagcg agtgggggcc cggctggaag tgcgccgggt tgctgtgaag gatctcggaa
      601 agaagcgcga cactccactc gatccggcca tattcacaac caagcccagc gaggtagtca
      661 gtgaccctga ggtcgggatc gtcgttgagg tcatgggtgg gatagagccc gctcggaccc
      721 tgatgatcga ggcgatgggc cgcggcaagc atgtcgtgac cgctaacaag gaactcctgt
      781 caactcatgg caaggatcta ttcgatgccg ccgagcacgc cggagtggac ctctatttcg
      841 aagcatccgt cgccgggggt atcccgataa tccgtcccct caaagagtcg ctcgctggcg
      901 agcggatcac tcgtgtgatg gggatcgtga acggcacgac caactacatc cttactcgga
      961 tgagcgagga ggctctcgac ttcaactccg cccttgcgga cgcgacgcgt ctgggttatt
     1021 cggaactcga cccgacagca gacatcgagg gtttcgacgc ggccgcgaaa ctggcgatcc
     1081 tctcgagcat cgcgttcaat gctcgtgtcg ttgcgagtga tgtctatagg gaaggcatct
     1141 cgagtgtcac ggcgcgcgat atcgcatacg gacatcaatt gggttacgag gtgaaactgc
     1201 tagccatcgg cgagaaggtc ggtggtgagg tgtctgtgcg agttcatccg gcgatgatcc
     1261 ccaagcacca ccccttggcg tcggtgcgtg agaatctgaa cgcggtgttc gtcgaaggcg
     1321 aacaggtcgg agagttgatg ttcttgggtc gcggggccgg tggcccccca acgggaacat
     1381 cggtggtggg tgatcttgtc gaggtggctc gcaacatggt ctccggcgga cgggggcctg
     1441 gatgcacctg ttaccgcgac gatgctcgta tccgttcgat ggatcagacc atgagccagc
     1501 actacatcct gctggaagtc ttcgaccgcc ccggtgtact tgccgaggtt gcgcggtcgt
     1561 tcgccgatca caacgtgagc atcaagagtc tctggcaaga gggctacggc gacgaagcaa
     1621 gtctcgtaat ggttacgcac agcgccgtcg aacatgacgt gcgtgaactg cttcgatctc
     1681 tcgagaaact cgagagtgtc gacgaagtgg ccgccagcat gcgggtccag tcctcggacg
     1741 agtagatgcc cgtctggcat ggggtcatcg aggagtaccg ctctcggctc ccggtcggcc
     1801 ccgactatcc cgtgatcacg ctgggagaag gtggaacccc cctcgttcac tcgaagcgac
     1861 tctcgcacca gactgggctt gaggtcttcc tcaaatacga gggcgcaaac ccgaccgggt
     1921 cattcaagga tcgcggcatg accgtggcgg tctcgatggc tgtgggttcg ggggccaaag
     1981 cgatcgcatg cgcctctacg ggtaacacct cagcttcggc agctgcctac gccgcaaggg
     2041 cggggatcat gtgcgcagtg ctcatcccgt cgggcaatgt cgctctcggc aaactagccc
     2101 aggctctcgt acatggcgcg aggacggttg aggtcagagg caatttcgat agggcactcg
     2161 agatcacgcg caaactcgaa gagtcttacc cggtgaccct cgtgaactcg atcaatcctt
     2221 accgtatcga aggccagaag acagcggcat tcgaggtttg cgatgcactc gacggggccc
     2281 ccgactacca cctgatgccc gtcgggaacg cgggcaacat aacttcctac tggaagggct
     2341 atcgcgaata cctgtcggac tcgatcatcg actcgacccc gcgcatgttc gggttccagg
     2401 ccgagggagc ggcccccatc gtgcggggat acccgatcga ttctcccaag acgatcgcca
     2461 ccgcgatcag gatcggcaac cccgcgtctt ggtcgcaagc tctcgaggcc gcagccgact
     2521 ccggcggcga gatacgcgcg gtgcgcgaca aggagatcct cgaggcatat c
//
LOCUS       DATDOZ010000051         5972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3436343, whole genome shotgun sequence.
ACCESSION   DATDOZ010000051 DATDOZ010000000
VERSION     DATDOZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3436343"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2544)
                     /locus_tag="VI541_00960"
     CDS             complement(79..2544)
                     /locus_tag="VI541_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HLF69506.1"
                     /translation="MDKWLRLVVGKAVGSVRRGAISQGSHDIVPGSSRSGVEVVRDTR
                     RALVGGFAAVTLVAMALAVLPLVRARGAESKVQMVGTQFVPAVTAIQTGDSVTWENAE
                     ATNYPVVIGVHNIISDDNAFTPSPPISPGKSWSLTFLKPGTFPYHCGVHPNQMKGVVK
                     VDGDPQPEPSGTAAKKSDGQSVTSDREGKLRKVELTVAERDITVLGVPTKWWTYDGTV
                     PGTTIRANVGDTLEITLKNPHNLPHAIHTHFEEYELSSDGSSMTASLPMVPHQEDDTV
                     GAVGGIVPGAAQQPGPKVGQNPIGPYDPRHDSDVARPGGSFTYRYKLTEPGTSWYHCH
                     VFEATDHIEKGLYGMIVVYPKGWTWKPLPPDPINGNTKAWVTNDLGEKLFEDLVIIGE
                     RNPAADSAVGLAGTGGVTGGPLHLANARGWNDPYIIGPVRSGQKILLHVGNIGESMHS
                     WHMHAHHFTRLEQFSEESSPGWFTRPLEHMHTKSVSPGEIFPMMITARRPGYWFNHDH
                     VVPQAYLGMVPWLHVLPAEVAASAVGSKTAEVKIVESKADDPESWKYDSSELNITVGD
                     KVVWRNTGKQDHTVTSASNSSEKFDSGTMKAGVAWEKTFSATGVFDYYCTVHPWMKGT
                     VSVAAVGAKAPAVSHSKSSGSHTSASAKRPSASGTKKVAPNIKIVEGKPDVPDSWVYD
                     PSEVTVSKGETVTWRNLGKLSHTVTTAGATKVFDSSGIAPGGTWKYTFNTEGVFAYIC
                     TLHPWMKGVVKVGDNGVVPVAMGGHGGEAMAVTTDEAAPKPVSHQDPQPLGTSPVALV
                     AFGFTGAAALGLAARTLLRRIKQ"
     gene            2713..4239
                     /locus_tag="VI541_00965"
     CDS             2713..4239
                     /locus_tag="VI541_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HLF69507.1"
                     /translation="MPFIDRLFSFEPQPRVVKRAWLIPLATAVVFDQLFWMRFPGLNV
                     PLFVAVLLGGGYGFMRLQGLRPTKSTRWLALSLLLFSSASTARDEPLTLILDYFVVLV
                     LAMLVADSASGKRWIDYGFREHVIAIVHVALAGIARGGVALLEPKETPGIEDGRDRWR
                     VLLRGSLITLPILLLFGSLLASADAVFASFITKPLSWLDSATFAEHVIRAVVILMVTY
                     VLFGVMLHAVRVRHDVGPPFAHGSADGRPARGVVEVAILLGSLNLLFGLFVGFQVRYF
                     FGGPSGFRRLGLTYSQYARKGFGELLAVGVFVLLLMLALNVLVRTQTRGERLLVASMA
                     SGLVVWTGLILISAFQRLLLYEDAYGFTRLRTYSHVFMLWVGIALTAILVLQWARAPH
                     RFALTLLIAGLGFTASLNILGVDAFVANKNIDRALQGAEFDADYLSGLSAEAIPVLIS
                     RRVELAPQDRDVVERGLVCDASRHRSHQIPWQSANHARLRAKRLLREFQARASAEVSC
                     "
     gene            complement(4267..4869)
                     /locus_tag="VI541_00970"
     CDS             complement(4267..4869)
                     /locus_tag="VI541_00970"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HLF69508.1"
                     /translation="MTTAPRVLVIDNYDSFTFNLVQYLLELGAEPLVARNDALSLDEV
                     TSLKVDGIVISPGPCTPHEAGITTELIRALGEQIPILGVCLGHQCIAQAYGAVVTRAE
                     RVVHGKRSLIHHDESHIYRGIPSPIEAARYHSLIVVPASLPEELVATAWTEDGHLMGI
                     RHKTYPVEGVQFHPESIITQHGHELMHNFLTSFQTLRDPV"
     gene            complement(5107..5937)
                     /locus_tag="VI541_00975"
                     /pseudo
     CDS             complement(5107..5937)
                     /locus_tag="VI541_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
BASE COUNT          997 a         1832 c         1865 g         1278 t
ORIGIN      
        1 agagtacata cgaactcgat atggcggtgg ttgcgcttct gctgtggacc ttacctactg
       61 tttcgtacag gacgctgatc actgcttgat ccgcctcagt agggtccgtg ctgcgaggcc
      121 aagcgccgcg gcgcccgtga agccgaaggc gacgagggcc accggagagg ttcccaaggg
      181 ctgcgggtcc tggtgactca ccggcttcgg tgccgcctca tcggtggtca ccgccatcgc
      241 ttcaccgccg tgtccgccca tcgcaacggg gaccacgccg ttatcgccaa ccttgaccac
      301 tcccttcatc catggatgga gggtgcagat gtaggcgaaa actccttcgg tgttgaaggt
      361 gtacttccag gttccgccgg gagcgatgcc gctcgagtcg aagaccttgg tcgctcccgc
      421 cgtcgtaacg gtgtgcgaca acttgccgag gttgcgccag gtcactgtct cgcccttcga
      481 gacggtgacc tccgaagggt cgtacaccca tgagtcgggg acatcgggct tgccctcgac
      541 gatcttgatg ttcggagcca ccttcttcgt cccgcttgct gatggtctct tggctgaggc
      601 actcgtatga gaaccggacg acttcgagtg cgagactgcc ggggccttcg caccaacagc
      661 ggcgaccgaa acggtcccct tcatccaggg atggacggtg cagtagtagt cgaagactcc
      721 ggtggccgag aaagttttct cccaggcgac tccggccttc atggttccgg agtcgaactt
      781 ctcggaagag ttcgatgcag aggtcacagt gtggtcctgt ttgccggtgt tgcgccagac
      841 gaccttgtct ccgacggtga tgttgagttc actggagtcg tacttccatg attcgggatc
      901 gtcagccttg gactcgacga tcttcacttc agcagtcttt gagccgaccg ctgacgccgc
      961 gacttccgct ggcagaacgt gcagccaagg caccatcccc aggtacgcct ggggcacgac
     1021 atggtcgtgg ttgaaccagt agccgggccg cctcgcagtg atcatcatgg ggaagatctc
     1081 gccgggcgaa acgctcttgg tgtgcatgtg ttcgaggggc cgggtgaacc agcccggcga
     1141 cgactcctca gagaactgct cgagtcttgt gaagtggtga gcgtgcatat gccacgagtg
     1201 catgctctcg ccgatgttgc cgacgtgtag cagtatcttc tgcccgctgc ggactggacc
     1261 gatgatgtaa gggtcgttcc atcctcgggc attcgccaga tgcagtggac cccccgtcac
     1321 gccaccggtt ccggcgaggc ccacagcgct gtccgctgca gggttgcgct ccccgatgat
     1381 gacgaggtcc tcgaagagtt tttcgccgag gtcgttggtg acccaggcct tggtgttccc
     1441 gttgatcggg tcgggaggca gcggcttcca cgtccacccc ttcgggtaga cgacgatcat
     1501 cccgtatagg cccttctcga tgtggtctgt cgcttcgaag acgtggcagt gataccacga
     1561 cgttcccggc tcggtgagct tatagcggta ggtgaaactg cctccgggcc gggcgacgtc
     1621 gctgtcgtgc cttgggtcgt agggcccgat cgggttctgt cccaccttcg ggccgggctg
     1681 ctgtgcggcc cccgggacta ttccacccac tgccccgact gtgtcatctt cctgatgcgg
     1741 gaccatcggc aacgaggcgg tcatcgatga gccatcgctc gagagttcgt actcctcgaa
     1801 gtgtgtgtgg atcgcgtgtg gaaggttgtg tgggttcttg agggtgatct cgagagtgtc
     1861 gccgacgttg gctcggatcg tggtccccgg caccgttcca tcgtaggtcc accacttcgt
     1921 ggggactccg agaacggtga tgtcgcgctc ggcaactgtc aactccacct tgcgaagttt
     1981 gccctccctg tcactcgtga ccgactgccc atctgacttc ttcgctgccg tccccgaagg
     2041 ttcgggctgg gggtccccat caaccttgac gacgcctttc atctggttcg ggtgaacgcc
     2101 gcagtggtac gggaaggtgc ccggcttgag gaacgtaaga gaccaggact tgcccgggct
     2161 gatcggaggc gagggggtga aggcgttgtc gtcggagatg atgttgtgga ctccgatgac
     2221 caccgggtag ttggtggcct cagcgttctc ccatgtgacg ctgtcgccgg tctggatggc
     2281 ggtaaccgca gggacgaact gagtgcccac catctgcacc ttgctctcgg ccccccgggc
     2341 cctcaccagt ggcaggaccg caagggccat ggcgaccagc gtaacggcgg cgaaaccgcc
     2401 aaccagggca cgacgcgtat cccgcaccac ctcgacacca gatctcgacg accccggaac
     2461 tatgtcgtgg gacccttgag atatggcgcc tctgcggaca ctgcctactg ccttgccaac
     2521 cacgagcctc agccacttgt ccatgcctac ccctcctctt gcgacggtca gacaagcatc
     2581 gccctcgagg gcaggcgctc ggtagggcct tccgactcga ttaactagtt agattgggct
     2641 actcccaagg tgccgttcgg ccgggccgat cggcaggtct ttcgggggcc aaggccgtag
     2701 tctgtggcca cgatgccctt catcgacaga ctcttctcct tcgagcctca acccagggtc
     2761 gtcaaacgtg cttggttgat ccccttggcc acggcagttg tcttcgatca gctgttttgg
     2821 atgagattcc ccgggctcaa cgttccgttg ttcgtggccg tgctgctcgg gggcggatat
     2881 gggttcatgc gccttcaggg cctccgacct acgaagtcaa cgagatggct cgccctttcc
     2941 ctactgctct tcagttcggc ctcgaccgcc cgcgacgaac ctctaaccct gatcctcgac
     3001 tacttcgtgg tcctggtctt ggcgatgctg gttgccgatt ctgcgtcggg gaagcgctgg
     3061 atcgactatg ggttccgcga acatgtcatc gccatcgttc atgtcgctct cgccgggatc
     3121 gcccgagggg gtgtcgcgct gcttgaaccg aaggagacgc ctgggatcga agatgggcga
     3181 gatcgctggc gtgtcctgct aagaggctct ctcatcactc tgcccatcct gctgttgttc
     3241 gggtccctcc tcgcgtcggc cgatgccgtg ttcgctagct tcatcaccaa gccgctgtcg
     3301 tggttggact ccgccacatt cgccgagcat gtgatccgcg cggtggtgat cctgatggtc
     3361 acatacgtcc tgttcggtgt gatgttgcat gcggtccgcg tgcgacacga tgtggggccc
     3421 ccgttcgcac atgggagcgc tgatggccgc ccggctcgcg gcgtcgtcga ggttgccatc
     3481 ctcttgggga gtttgaacct cctattcggc ctcttcgtcg gcttccaagt gcgctacttc
     3541 ttcggggggc ccagtggctt ccgcagactt ggcctgacct attcgcagta cgcgaggaag
     3601 ggtttcggtg agctgttggc ggttggtgtg ttcgtcctcc tcctcatgct ggccctcaac
     3661 gtgctggtca gaacgcagac gaggggggag cggttgctag ttgcgtctat ggcatctggg
     3721 cttgtcgttt ggacgggcct gatcctcatc tcggccttcc aacggcttct cctctatgag
     3781 gatgcttacg gattcaccag gctgcgcacc tactcgcacg tgttcatgct gtgggtgggc
     3841 attgcgctca ctgccatcct ggtgcttcag tgggcgcgcg ccccgcatcg gttcgcgcta
     3901 acgctcttga tagcgggact cggcttcacc gcgtcgctga acatcctggg cgtcgatgcg
     3961 ttcgtggcga ataagaacat agaccgagcg ctacaggggg cggagttcga cgcggactac
     4021 ctgagcgggc tctctgccga ggcgataccc gtattgatca gccgacgagt ggaactggcc
     4081 ccacaagacc gagacgtcgt ggagcggggg ctcgtctgcg atgccagtcg ccataggagc
     4141 caccagatcc cgtggcagtc agcgaatcac gcgcgcctac gcgcgaagcg gctcctgcgg
     4201 gagttccagg cgcgggcttc ggcggaagtc tcctgctagg tctcgtcaac atagacgtct
     4261 ccgatgttag accggatcac gaagtgtctg gaagctagtc aggaagttgt gcatcagttc
     4321 gtggccatgc tgggtgatga tcgactcagg gtggaattga accccttcga cggggtaggt
     4381 cttgtgcctt atccccatca gatgaccatc ttcggtccag gcagtagcaa caagttcctc
     4441 aggaagactc gccggtacca cgatcagcga gtggtacctg gccgcctcga tggggcttgg
     4501 gatgccccgg tagatgtgac tctcgtcatg atggatgagc gatcgtttcc cgtggacaac
     4561 ccgctcggcc ctcgtcacca cagcgccata ggcctgtgcg atgcactggt gaccaagaca
     4621 gactccgagg atggggatct gctcgcccaa ggctcggatc agctctgtgg tgatccctgc
     4681 ctcgtgaggg gtgcaggggc ctggcgagat gacgatcccg tcgaccttca gcgatgtcac
     4741 ctcgtcgagg ctgagcgcgt cgttgcgagc gactagcggt tctgccccca actcgagaag
     4801 gtactgaacc agattgaagg tgaaggagtc gtagttgtcg atcaccagta cgcggggcgc
     4861 agtggtgatg ccatcgactc caaggtgtat gggcgtgtac tgccggacgg cctggctcgg
     4921 agggttatga gaaccccgtc cctgcttcac gcggttggcc tgctcgtcga cgaaccgatc
     4981 gagttccgta ccgatcatgg tggttatggg gccccgcgcc caagagttca gcgatgcgca
     5041 ctcactgacc cactcgacgc ggcgcagaga ccccgtggtg aatacctcgt cagcagttct
     5101 cagctcttcc aaagagatgg gttcctctcg caccgggatc gagttcagcc gcaggagcga
     5161 gagcacccgg cttcgcgtga tgcccggcag gatccgatcg tcgcatgggg gcgtcaccac
     5221 gctcccatcg aagactgcga agaggttggc tcgcgcggtt tcgagcacga ggcctgcttc
     5281 atcgactatc aacaagtgct cgtcctcggc agggtcggtc atgcgtggtg cgatcgcctc
     5341 gcgatcgcac cacttatggg ggccgagccc gcctgagaca acgaacggag tcagggcggt
     5401 gccaactctc tgcccgtcgg cgaaccccgc aggcatcggt aggatcgctg cagttagcga
     5461 gacttcgccg cggtggtcgg gtttcgcaac gagtctcaat cgggcgattc cttcagctgc
     5521 tcgtgacgcg gccttcatcg caagttgtgc ggcccccggg tctagagcga ctccgtacaa
     5581 cgcacgaaca ctcgacttca tccgtgcgag gtgggcgtgc agctcgatac acgctccgcg
     5641 tctcacgagg atggtctcga agaccccgag ctcctgacga accgaacgac gcagcgccga
     5701 caccgttgtg ggttgcggca cagccaggtg atcactcatc gaggctccga tcgcaccgat
     5761 cagaggggcg gccttcacat aacactctct gcgctcgcct tcgggatcgg aatcggcaac
     5821 gatccccccg ccaaccccca accagatcct gccctccgcg atctcgaatg tgcggatcgc
     5881 gacgttgaat tcgagttgat cgagggggct cgcgtatccg atcgctcccg tgtacacctc
     5941 gcgcgcggtt gtttcgagtt catggacgat ct
//
LOCUS       DATDOZ010000052         4996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2141986, whole genome shotgun sequence.
ACCESSION   DATDOZ010000052 DATDOZ010000000
VERSION     DATDOZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141986"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="aroB"
                     /locus_tag="VI541_00980"
     CDS             <1..1059
                     /gene="aroB"
                     /locus_tag="VI541_00980"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HLF69509.1"
                     /translation="RTISVELGRDSYDVVVGEGALDELGPRVATMGAKRVAIITDSHV
                     SDIAKTKVEPLLAEHLPTHRIELPAGENSKSLASASEIYERLLDLEMRRDDLVVTIGG
                     GMIGDLGGFVASTFQRGVQLVHVPTTLLAQVDAAIGGKTAVNLARGKNLVGTFYQPAL
                     VVCDTSLLVTLPDRQFRSGAAEVVKYALTFGGGLLDRLRSDPWPPSGLELDETVALCV
                     DAKARVVSGDRADRGERLFLNYGHTLGHAIEAAGGYERWTHGEAIALGMVFASGVSHA
                     MGLVGEDVVQLHRHLLEKVGLPVRDEIPRLQTESAMTLDKKHAQRQRWVLLGGVGRPV
                     IRDDVPDPAIEAGFEAIS"
     gene            1056..1499
                     /gene="aroQ"
                     /locus_tag="VI541_00985"
     CDS             1056..1499
                     /gene="aroQ"
                     /locus_tag="VI541_00985"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLF69510.1"
                     /translation="MKTIFVLNGPSLNLLGERDPEVYGSSTLADVESLCRETAAGQQL
                     ETVFRQTDEEGELIGWLNEAGIKADGVVINPAAFTHYSVAVRDAIAALECPVIEVHLS
                     NIYAREEFRAHSVVSGVVKGVIAGLGITGYALAIEALAEVIGRDT"
     gene            1499..2575
                     /locus_tag="VI541_00990"
     CDS             1499..2575
                     /locus_tag="VI541_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HLF69511.1"
                     /translation="MTFQGRTAGLQQRIVREEVDGLLVTNLINVRYLTGFTGSSGALL
                     LSPDAAVFFTDGRYIAQSAQEVVGAEIEIYTTQPQYAQQLSERSREMGISRLGREETL
                     TLADAVAYSKRLPEVEFVSANGWVEALRAVKDSTELASMRVAAEMADAGLAYILERVE
                     VGRTESDLALDLEFFMRRNGASDVSFDVIVAAGERSALPHAHPTDKPVEKGGFLLFDL
                     GCVYQGYCSDLTRTVVIGPSEQRHREIYDIVLGAQEAALTTLGPQVVVGEVDSAARTL
                     IADAGYGEAFSHGLGHGVGLEIHEAPTLRRDEEQVLLPGNVVTVEPGIYIEGFGGVRI
                     EDLVIIGESGAEIISKSPKTFTVL"
     gene            2612..3172
                     /gene="efp"
                     /locus_tag="VI541_00995"
     CDS             2612..3172
                     /gene="efp"
                     /locus_tag="VI541_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLF69512.1"
                     /translation="MISTNDMRPGMTLDLDGDLFTIVNYQHHKPGKGGAMVRTKLKNV
                     KTGAVTDRTFRADEKVELARLDKREMQFLYSDEESYVFMDNESFEQMHIDKDFVGDAS
                     NYLKEGDKVQIPLYQGVPIGLDLPTTVDLTITETDPGLQGDRSSAGTKPATLETGAIV
                     QVPLFIVVGETIRVDTRTGSYVTRVR"
     gene            3176..3559
                     /gene="nusB"
                     /locus_tag="VI541_01000"
     CDS             3176..3559
                     /gene="nusB"
                     /locus_tag="VI541_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLF69513.1"
                     /translation="MSRRRGSRKLALDVLYEHDVSGAPLSEILERHRSNPGFEMTREI
                     VDGVESNIDSIDATITSLSEQWAIDRMPVVDRNLIRIGVYEILYASDVPSAVAIDEAV
                     ALAKIYSTEDSGRFVNGLLAAVAKD"
     gene            3702..4238
                     /gene="pyrR"
                     /locus_tag="VI541_01005"
     CDS             3702..4238
                     /gene="pyrR"
                     /locus_tag="VI541_01005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HLF69514.1"
                     /translation="MSSDRILLEASDISRAIRRIAHEIVERNKGASELAIVGIRKRGL
                     PLAERLGAEIAAIEGRAPDVGALDVTFYRDDFAIRSPEQIGSTVFPFDIEGRTVVLVD
                     DVLFTGRTIRAAIDALVDFGRPRAVQLAVLIDRGHKEIPIRADYIGKNIPTSIKEDVK
                     VMLVETDGIDAVQIVKEP"
     gene            4238..>4996
                     /locus_tag="VI541_01010"
     CDS             4238..>4996
                     /locus_tag="VI541_01010"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HLF69515.1"
                     /translation="MRHLLSIDQLSPEDIELILATAESFREVGTRKIKKVPILRGRTV
                     CNLFFEASTRTRISFELAAKRLSADVINFTADAKTSVSKGESFKDTAMTLQAMGVDAI
                     VVRHSSSGAAQRLAEWVEARVINAGDGSHEHPTQALLDLYSIRERFPSFEGLRVAIVG
                     DVIHSRVARSNIKAMIKMGAQVIAVGPPTLMPRTVESWGAEVSTEIDDVLPKVDVVYL
                     LRVQRERQNEQLFPSVREYAALWGLDRRRVELLKP"
BASE COUNT         1001 a         1458 c         1585 g          952 t
ORIGIN      
        1 aggacgatct cggtggaact gggccgggac tcgtacgacg ttgtcgtcgg agagggggcc
       61 ttggatgaac tcggcccccg ggtggcgacc atgggggcca agagggtggc gatcatcact
      121 gattcacatg tatccgacat cgcaaagacc aaggtagagc ctctgctcgc cgaacacctg
      181 ccgacgcacc ggatcgagct gccagcgggg gagaacagca agtcgctcgc atctgcatcc
      241 gagatctacg agaggcttct tgatctagag atgcgtcgcg atgatctggt cgtcaccatc
      301 ggcggcggga tgatcggcga cctaggtggg ttcgtcgcct caacctttca acgcggggtc
      361 caattggtcc atgtacccac aacgctgctt gcgcaggtcg atgccgcgat cgggggcaag
      421 actgccgtca acctcgcgcg cggaaagaat ctcgtgggta ccttctatca acccgcgctc
      481 gtggtgtgcg atacatcgct cctggtaact ctgccagaca gacagttccg ctccggagca
      541 gcggaggtcg tcaagtacgc gctcactttc ggcggaggtc tgctggaccg cctacggtcg
      601 gacccttggc ccccgtccgg tctcgagctg gacgaaacgg tggccctctg tgtcgacgcg
      661 aaggcgcgtg tagtctcggg cgatcgagcc gatcggggcg agagactctt cctcaactac
      721 ggccacacgc tcggtcatgc catcgaggct gcgggaggat acgagaggtg gacgcacgga
      781 gaggcgatcg cacttgggat ggtcttcgcc tcgggggtgt cccatgcgat gggtctggtc
      841 ggcgaagatg tcgtccaact tcatcgtcat ctccttgaga aggtcggtct gcccgtgcgt
      901 gacgagatcc cacggctgca gacggagtca gctatgaccc tcgacaagaa gcatgcgcag
      961 cggcagcgat gggttctgct cggaggcgtg ggtcgtcccg tcatccgcga cgatgtcccc
     1021 gatccggcca tcgaggctgg gttcgaagcg atctcatgaa gacgatcttc gttctgaacg
     1081 gtccaagcct caacctgcta ggcgaacgcg atcccgaggt gtacggctcc tcgacgctgg
     1141 cggatgtcga gtcgctctgt cgagagaccg ccgcgggaca gcaactcgag actgttttca
     1201 ggcagaccga cgaggaggga gagctcatcg ggtggctgaa cgaggccggt atcaaggcag
     1261 acggagtcgt gatcaatccg gcggctttca cgcactactc ggtcgcggtg cgcgatgcca
     1321 tcgcagctct cgagtgcccg gttatcgagg ttcacctctc gaacatctat gctcgcgagg
     1381 agttcagagc tcactccgtt gtgtcaggcg tagtcaaggg agttatcgcc ggattgggca
     1441 tcactggtta cgctcttgcc atcgaagctc tcgcagaggt gatcggaagg gacacgtgat
     1501 gacatttcaa ggtaggactg cgggtctgca gcagcgtatc gtgcgtgaag aggtcgatgg
     1561 cctcctcgta actaacctga tcaatgtccg gtatctgact ggtttcaccg gatccagcgg
     1621 ggccctactg ctctccccag acgcagcggt tttcttcaca gacggccggt acatcgccca
     1681 gagcgcccag gaggtcgttg gggctgagat cgagatctat acaacccagc ctcagtacgc
     1741 gcagcagctt tcggagcgtt cgcgtgagat ggggatctcg cggctgggtc gagaggagac
     1801 tctgacgctc gctgatgcag tggcttattc gaaaaggttg ccggaggtcg agttcgtgtc
     1861 cgcgaacggg tgggtcgagg ccctgcgagc ggtcaaggat tcaacggagt tggcctcgat
     1921 gcgcgttgcc gccgagatgg cggacgctgg gttggcctac atcttggagc gagtagaggt
     1981 cggcaggacc gaatcagatc tggccctgga ccttgagttc ttcatgcggc gcaatggtgc
     2041 aagcgatgtg tcgttcgatg tcatcgtagc agcgggcgaa cgcagtgccc ttccgcatgc
     2101 ccaccccacc gacaaaccgg ttgagaaggg cggtttcctg ttgttcgacc tcggctgcgt
     2161 gtatcagggg tactgctccg acctgacccg caccgtggtg attgggccga gcgagcagcg
     2221 acaccgcgag atctacgaca tcgtgctggg ggcccaggag gcggccctca ctacgctcgg
     2281 cccccaagtc gtcgtgggag aggtcgactc cgctgctcgg accttgatcg cggacgcggg
     2341 gtatggcgag gccttctctc atggcctcgg gcacggcgtc ggtctcgaga tccacgaggc
     2401 gcccaccctg aggcgcgacg aggaacaggt cctcctgcca ggcaatgtcg tcacggtcga
     2461 acccggtatc tacatcgagg gtttcggtgg tgtgcggatc gaagatctgg tcatcatcgg
     2521 cgagtcgggg gccgagatca tcagcaagtc acccaagacc ttcaccgtcc tgtaaccttc
     2581 acatccgctt caagatggaa tgaggtagtc gatgatctca acgaatgaca tgcgtccggg
     2641 catgactttg gacctcgatg gtgacctgtt caccatcgtg aactatcagc accacaaacc
     2701 cggcaagggc ggggcgatgg tgcgcaccaa actgaaaaac gtcaagacgg gagcggtcac
     2761 cgatcgcaca ttccgtgccg atgagaaggt cgaacttgcg cgcctggaca agagagagat
     2821 gcagttcctc tactcagacg aagagtccta tgtctttatg gacaacgagt cgtttgagca
     2881 gatgcacatc gacaaagact tcgtcgggga tgcgtccaac tatctcaagg agggcgacaa
     2941 ggttcagatc cccctgtatc agggtgttcc gatcggcctc gaccttccaa cgaccgtcga
     3001 cctcaccatc acagaaacgg atcccggcct tcagggcgat cgcagttcgg ctgggaccaa
     3061 gcccgcgacg ctcgaaaccg gcgccatcgt ccaggttccc ctcttcatcg tcgttggaga
     3121 gaccatccgg gtcgatacgc ggacgggcag ctacgtaacg cgagtcagat agcggatgtc
     3181 ccggcgcagg ggtagccgga aactcgcgct cgacgtcctg tacgagcacg acgtgtcggg
     3241 ggcaccactg agcgagatcc tcgaacgaca tcgctcgaac ccaggcttcg agatgacccg
     3301 agagatagtg gacggtgtgg agtcgaacat cgacagcatc gatgcgacga tcacttcgct
     3361 gtctgagcag tgggcgatcg accggatgcc tgtggtggat cgcaacctca tccggatcgg
     3421 cgtctacgag atcctctatg cgagcgatgt cccaagcgcc gtggccatcg acgaggccgt
     3481 ggccctcgcg aagatctact caaccgagga ttccggcagg ttcgtgaacg gcctcctggc
     3541 ggccgtggcc aaggattgag gaaccccttc ttgcggcccc cagggcgcgc gaggtaaggt
     3601 agtcgggaac tacttccttt aagactggtc ctgagaggcc agaaaggggc acagatggct
     3661 gcacactgct gcgccgtacc gcttgcggac ggcgtttttt gatgtcttcc gaccggatcc
     3721 tcctcgaagc ttcagacatc tcgcgggcca tccgcaggat cgctcacgag atcgtcgagc
     3781 gaaacaaggg ggcatccgag ctggccatcg ttggcatccg caaacgcggc cttccgttgg
     3841 cagagaggct gggggccgag atcgcggcga tcgaaggcag ggcccccgat gtcggtgctc
     3901 tcgacgtgac cttctaccgc gatgacttcg cgatccgcag tcccgagcag ataggttcga
     3961 ccgtcttccc attcgacatc gaaggacgca ctgtcgttct ggtcgatgac gtcctcttca
     4021 cgggacgcac catcagagcc gccatcgacg cgctggtcga cttcggacga ccaagagcgg
     4081 tgcagttggc cgttctcatc gatcgaggac ataaagagat ccccatccgg gccgactaca
     4141 tcggaaagaa catcccgaca tcgatcaagg aggatgtcaa ggtgatgctc gtcgagacgg
     4201 acggcatcga cgccgtccag atcgtgaagg aaccatgatc cgccacctgc tctcgataga
     4261 tcagctgagc cccgaggata tcgagctgat cctggccacc gccgagtcat tcagagaggt
     4321 aggcactcgc aagatcaaga aggttccgat cctgcgcggc aggaccgtct gcaacctctt
     4381 cttcgaggca tcgacccgaa cgcgtatctc cttcgagttg gccgcgaagc ggctgtcggc
     4441 tgacgtgatc aacttcacgg cggatgcgaa gacatcggtg tcgaagggcg aatcattcaa
     4501 ggacaccgct atgactcttc aagcgatggg cgtcgacgcc atagtggtgc ggcactcatc
     4561 ttcgggtgcg gctcagcgac tagccgagtg ggtcgaggct agggtcatca atgctggaga
     4621 tggttcccac gagcacccta cgcaagcgct gctcgacctc tactcgatac gcgaacgctt
     4681 cccgagcttc gaaggtttga gagtggcgat cgtgggagac gtgatccatt ccagggtggc
     4741 acgttcgaac atcaaggcga tgatcaagat gggcgcacag gtgatcgcgg tcggcccccc
     4801 aacgctcatg ccccgaaccg tcgagagctg gggggccgag gtatcgaccg agatcgacga
     4861 tgtactgccc aaggtggatg ttgtgtacct gcttcgggtc cagagggaac gacagaatga
     4921 acagctcttc ccgtcggtgc gcgagtacgc ggccctctgg gggctcgatc gaagacgagt
     4981 cgaactcctc aagccc
//
LOCUS       DATDOZ010000053         3448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_561942, whole genome shotgun sequence.
ACCESSION   DATDOZ010000053 DATDOZ010000000
VERSION     DATDOZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561942"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VI541_01015"
     CDS             <1..316
                     /locus_tag="VI541_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF69516.1"
                     /translation="GVQAAGSVHHIAWACSDEEHEAWRRRLSDSGAEVTPIIDRTYFR
                     SIYFREPNGILFEIATRGPGFTVDESAADLGTGLQLPPQYESMRSNLESQLTPLRNPR
                     AL"
     gene            357..989
                     /locus_tag="VI541_01020"
     CDS             357..989
                     /locus_tag="VI541_01020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HLF69517.1"
                     /translation="MDFPGEQVFKYMRSLHDKLDEPVLVEMEAVAAEAGFPIVGRVVG
                     AALETLARTIGAARVFEMGSGFGYSGYWFARAVGESGEVTLTDGDPENALKAKDYLTR
                     AGLWDRCRFEVGDAIEALGRADGEFDVIYCDIDKGDYPAAFKVARERVRVGGLYICDN
                     VLWSGRVAADDADAWTVAIRQHNLDIYGDESFLPVILPIRDGVIMALRTK"
     gene            complement(1001..1621)
                     /locus_tag="VI541_01025"
     CDS             complement(1001..1621)
                     /locus_tag="VI541_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF69518.1"
                     /translation="MATGEASILEAQGSWSGSISVTEASDLISRARIGDKDAFAALYR
                     EYVQTVHRFVAARVKNPQKAEDIVAETFVKALRNINRFEFRGIEFSAWLVRIARNLIM
                     DNARSATSNLEVLQDETPEGAPEADTGDLAMAAIEAERLRGALDQMIPDHRTVLELRF
                     VKGYPVTEVSQLMAKSEGAVRVLQYRALRAMKRYLEKNAPDLAPAS"
     gene            1780..2421
                     /locus_tag="VI541_01030"
     CDS             1780..2421
                     /locus_tag="VI541_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF69519.1"
                     /translation="MSDLAEGALIRVVVVDDHPSYAKGLQMLLASELANIDVVGLATS
                     GADGLDVVGKLLPDVVLMDIRMPGLGGIETTRLIKEQFPVVKVVMLTVSEEDQDVYDA
                     MRLGASGYLPKQIEVEDLVSAIRSIHAGQVVISPLVAGKLLGHSREEDVPLTDGDREL
                     LRLVAEGYDNAKIAETLSVSESTLKRNLRNILEKLQLHNRVQAAVYAAKKGWI"
     gene            complement(2448..>3448)
                     /locus_tag="VI541_01035"
     CDS             complement(2448..>3448)
                     /locus_tag="VI541_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69520.1"
                     /translation="RRNGIPVGTSMIGPPVRQGLCELQPNFPTCSIFGVAFAANTIPG
                     HQGFWRSLVQGGEGVLATMSVLVTAHSVDGGVASATRSFTFDPQAPEFGPVTRALEPG
                     KQSVSGTVGDNLGLSRVDITYFSGPESTARSFGQSRPIEQILDEINVTDEPLCPGEAW
                     NNECGQEILFLYNYSAANADTERVVRKKTLCDRDCGQAFNWLDSPTLSPGDYTVLVGA
                     SDVAGNLASHLLSLSIPYQGCTIVSHVTATSTNGSVSPAKDQCSYIATGPGTFYSASN
                     FFSIQTEPGDGQWHGQDQRPAPFGRGNSGRINVAPGTTVRIRFGGGEYGAVRVRDD"
BASE COUNT          653 a         1002 c         1087 g          706 t
ORIGIN      
        1 cggggtgcaa gccgccgggt ccgttcacca catcgcgtgg gcatgttcgg atgaagagca
       61 tgaagcttgg aggcgcagac tgagtgattc gggggcggag gttacaccga tcatcgaccg
      121 aacctacttc cgctccatct acttccgcga acccaacggc atcctgttcg agatcgcgac
      181 ccggggcccc ggcttcacag tcgatgagtc ggccgcggac ctcgggactg gtctgcagct
      241 tccgcctcag tatgaatcga tgcgctctaa cctcgaatca caactcacgc cgctgcgaaa
      301 cccaagggcg ttgtgacgcg tgcacttacg gccatcgcta tgaaagactg acgccgatgg
      361 actttccggg tgagcaggtc ttcaagtaca tgcggtctct gcatgacaag ctcgacgagc
      421 cggtcctcgt agagatggag gccgtcgccg ccgaggctgg ctttccgata gttggacgcg
      481 tcgtgggggc cgctctcgag acgcttgcgc gcacgatcgg tgccgcacgc gtattcgaga
      541 tggggtccgg gttcggatat tcgggttact ggttcgcaag agcggttggt gagtccggcg
      601 aggtgacgct cacggatggg gaccccgaaa acgcgctcaa ggccaaggac tacctcacgc
      661 gagcgggtct ttgggataga tgccgcttcg aggtcggcga cgcgatcgag gccctcggtc
      721 gggccgatgg agagttcgac gtcatctact gcgacatcga caagggggat tatccggccg
      781 ccttcaaagt cgcgcgggag cgtgttcgag tgggggggct ctacatctgc gacaacgtcc
      841 tatggtctgg acgcgttgcg gccgacgacg cagatgcgtg gaccgttgcc atacgtcagc
      901 acaatctcga tatctacggt gatgagtcgt tcctgccagt gatactgccg atccgcgatg
      961 gcgtgatcat ggccctgcga acgaagtagc agggcccgtt ctagctggcg ggagccaggt
     1021 cgggagcgtt cttctcgagg tagcgcttca tcgcccgaag agcccggtac tgcagcaccc
     1081 ggacggcccc ctcgctcttg gccatgagtt gtgaaacctc ggtcaccggg tagcccttta
     1141 cgaagcgcag ttccaggacc gtgcggtgat cggggatcat ctggtcgagt gctccacgaa
     1201 gacgctcggc ctcgatggcg gccatggcga ggtccccggt gtcggcctcg ggggcccctt
     1261 ccggtgtctc atcctgcagg acctcgaggt tcgaggtggc agagcgagca ttgtccatga
     1321 tcaggtttcg tgcgatccgc acgagccatg ccgagaactc gatccctctg aactcgaaac
     1381 ggttgatgtt gcgaagagcc ttgacgaagg tctcggcgac gatgtcctca gccttctggg
     1441 ggttcttcac cctggcggcg acgaatcgat ggaccgtctg aacgtactca cgatagaggg
     1501 ctgcgaacgc atccttgtct ccgatgcggg ctcggctgat gagatccgat gcctctgtga
     1561 ccgagatcga gccgctccag ctgccctgtg cctcgaggat gctcgcttct ccagttgcca
     1621 tcgaaccccc tacgtagtcc atgtgtacta gtcatttcga actagtgatg ggccatcttg
     1681 aggcgacgca ggtgaaacga tccctgcggt tcacaggttg accttcgggg ctcacggacc
     1741 tgaaccggat atccgctcgg ttgattagat aatcgggata tgtccgacct cgccgaaggc
     1801 gctctgatca gagtcgtggt tgtcgatgac cacccttctt atgccaaagg gcttcagatg
     1861 ctgcttgcat ccgagttagc caacatcgat gtggttggtc tcgcgacctc gggggccgac
     1921 ggactcgatg tcgtcgggaa gctccttccc gatgtggtcc tgatggatat caggatgccg
     1981 gggctagggg gcatcgagac cacccgcttg atcaaggaac agttcccggt ggtcaaagtt
     2041 gtcatgctca cggtttctga ggaggaccag gatgtgtacg acgcgatgcg cctaggggcg
     2101 agcggctacc ttcccaagca gatcgaggtc gaggacctcg tgtccgccat ccggtcgatc
     2161 cacgcgggcc aggtggtcat ctcacccctt gtggctggga agctgcttgg gcactctcgc
     2221 gaggaggatg ttccgctgac cgatggcgac cgtgagcttt tgagactcgt ggctgagggg
     2281 tacgacaacg ccaagatcgc ggagacactc tccgtcagcg aatcgacctt gaagcgcaat
     2341 ctccgcaaca tcctagaaaa gctgcagctg cacaaccgcg tgcaggcggc ggtctatgca
     2401 gccaagaagg gctggatctg acctagatcg tgcatcgatc tgggggatca gtcgtcgcgc
     2461 actcgaactg caccgtattc gcctccgcca aaacgaatcc tcacggtggt tcctggggcg
     2521 acgttgatcc gaccagagtt gccgcggcca aacggcgcgg gacgttggtc ctgcccgtgc
     2581 cactggccgt cgcctggctc cgtttggatc gagaagaagt tggacgcgga atagaacgtg
     2641 ccgggcccag tagcgatgta tgagcattga tccttcgcgg ggctcacact gccattggtg
     2701 ctagtcgcgg tcacatggct gacgatcgtg cacccttgat atgggatgct caggctaagc
     2761 aggtggcttg ctaggttccc agctacatct gaagcgccaa cgaggacggt gtagtcgccc
     2821 ggtgagagcg taggactatc cagccagttg aacgcttgcc cgcaatcgcg gtcacaaagg
     2881 gttttcttcc tcacgactct ttcggtatca gcgttggcgg cagagtagtt gtaaagaaag
     2941 aggatctcct gaccgcactc gttgttccat gcttcgccgg ggcagagtgg ctcatcagtg
     3001 acattaatct cgtcaagtat ctgctcgatg ggccgactct ggccgaatga acgtgccgtc
     3061 gactccggcc cactgaagta ggtgatgtcg acccgagaca agcccaggtt gtccccgacg
     3121 gttccgctga ccgactgctt ccctggttcg agtgctctcg tgacgggccc gaactcgggg
     3181 gcttgcgggt cgaaagtgaa ggatcttgtt gccgatgcga ctcccccgtc aacggagtgt
     3241 gcagtgacca gaacactcat cgtagcgagc acgccctccc caccttggac gagagacctc
     3301 cagaagccct gatgaccggg gatcgtgtta gccgcgaagg cgaccccgaa gatcgagcat
     3361 gttggaaagt tcggctgcag ttcacagaga ccctggcgaa ccggtggacc gatcatcgac
     3421 gttcccacgg ggatcccgtt acgtcgcc
//
LOCUS       DATDOZ010000054         4383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_302073, whole genome shotgun sequence.
ACCESSION   DATDOZ010000054 DATDOZ010000000
VERSION     DATDOZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302073"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..2163
                     /locus_tag="VI541_01040"
     CDS             70..2163
                     /locus_tag="VI541_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69521.1"
                     /translation="MRAQAAVPTVEVEMFSIKSRQDEGRYALDEEVVLESLRTINSPK
                     VLGSALRSLLTRALAICGADEGSVMLLGDDSKLRISSSVGLPPEVIHNTVLELGAGIA
                     GKVAQTGTPLLLNGEIPQIDEGGLAPRKPVYSALSVALKSGNTTVGVLNLNITTKERA
                     FSERDLSVASIFAEEAAIAIEKSRLLQKSESRAKALTELLAASRALLDTVEPEALLTN
                     VLNQARSLSGIAGGIAGIVDGSEYKMCVFSGPERDAVTAFIHESLAVPGCLTGGVSVR
                     ASDTQLGPHLGPSDWITSWSLPTGDSTRLVLFLVGERLNEDHSGILSTFLATASLALR
                     NANLADGLSKRSNELVSIVYSMTNPVIVADARGNLVTSNPAAEELFGFSTVFQVGHHI
                     NNVLNDPSLVSLLTEDVDDTVETVAGVTERRVWKAKSSRIRSGHSQLSGRVLVLEDVT
                     AEREFAEMKANFVAVVGHELRTPMTLIKGFLRTLVRKGDLMTEAQRGESLRTLESQAV
                     RLERLIEDLLFVSRIENSRPELFAEHGNLMHVIHDLLLEFQTREPGRHLSIQGPDRLD
                     LVFDKTRVEQILVHLLDNACKYSDPSTPVVVAVHEHLDHVSVAVTDRGMGILSGDLED
                     LFRLLHQVDGTSTRAAGGLGVGLYICKSLVESHGGHMEVNSLWGKGSTFTFTIPKGIE
                     VASRAITATLTDPQA"
     gene            complement(2154..3083)
                     /locus_tag="VI541_01045"
     CDS             complement(2154..3083)
                     /locus_tag="VI541_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HLF69522.1"
                     /translation="MAESTHDKFRRLIAMINLVQERGAIEVQELVDIFQVNKRQLMND
                     LDMIQMCGLPGYSPGDLIEVVLEGDLVSIRSADYFARPLNLAPSEAIMLFAGVQALAA
                     TGDADKNLRSAMEKLAGAMGEHVAGRLAVDVDQLEEVAVIRGAMHDHRRIHLIYQSGS
                     KEEITERKVDPHRLFVWEGIWYVWAYCHRAQADRVFRVDRIRSLELLEEIYVPSQQDA
                     TRYSGIDFEGPPGQELTLEVSPESSWVIEKLRAKVVGETEDGWTSVSVDGADTVWMER
                     MLLMLGPRVRNVEPVSVAERVRALAQKVLNSYA"
     gene            complement(3076..4032)
                     /locus_tag="VI541_01050"
     CDS             complement(3076..4032)
                     /locus_tag="VI541_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HLF69523.1"
                     /translation="MSTKKAERVMNLLIMLLAARAPVTSAEIKTTIPGYDQENDDTFR
                     RMLERDKQDLREMGIQVDFAPTDIWEVEKGYRIVKEDFYLPDVELTSAEAAALWLAVT
                     MVARENDSVARNALLKLAGGVPEGHESSGLSADVGLSDPNLTIAFEAVSRRRSVTFQY
                     RKGSDAERRTLDPYNLKNRRGAWYLIGRDHDRDATRAFRLDRIRGTMLVMDPGGEPSF
                     SIPDDLDLSHVLDSPPFVVGDDVFAVARVRFEPTLAWWVGRTTPWLRLEDHPDGSAEA
                     DLTVTNVNGFIRWLFWFGEDAHLLSPQHLIDEVRSELESRLG"
     gene            complement(4032..>4383)
                     /locus_tag="VI541_01055"
     CDS             complement(4032..>4383)
                     /locus_tag="VI541_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HLF69524.1"
                     /translation="VAALRVSFADARERHRGVSHHTIGALRFGTTAGCTVAVPDLDVA
                     ELSAVMEDLIETGVAQMHDVRIVDAWETLTVLSDLDLRVTTMGRSAKDDPAFFKAAGA
                     AGLVAGQMVLGSYE"
BASE COUNT          912 a         1320 c         1257 g          894 t
ORIGIN      
        1 ttggccatac ccacggtagt catcgggact agtcctaacc ttcctggcat ttcctgccga
       61 tattcctgcg tgagggcaca ggccgctgtg cccacggttg aggttgagat gttctcgatc
      121 aagagccgac aggacgaggg aagatacgca cttgatgaag aggttgtcct cgagtcgttg
      181 aggacgatca attcacccaa ggtcctcggc agcgcacttc gatccctact tacgcgcgct
      241 cttgcgatat gcggagcgga cgagggctcc gtcatgcttc tgggcgatga ctcgaagctt
      301 cggatctcgt cgagcgttgg actcccgccg gaagtcatcc acaacaccgt ccttgagctc
      361 ggggctggga tagccggcaa ggttgcccag actggtaccc ccctgctgct caatggtgag
      421 ataccgcaga tcgatgaagg gggccttgcc ccgaggaagc cggtctactc tgcgcttagt
      481 gtcgcgctga agagtgggaa cacgacagtg ggcgtcttga acctcaacat caccaccaaa
      541 gaaagagcat tctccgagcg agatctgagt gtggcgtcca tcttcgcgga ggaagcggcc
      601 atagcgatag agaagtccag gctgcttcag aagtcggaga gtagagcgaa ggctctgacc
      661 gaactgcttg cggccagcag ggccttactg gacaccgtcg agcccgaggc actcctcacg
      721 aacgtcttga atcaagcaag gtcactctcg ggaatcgccg gcggtatagc aggcatagtg
      781 gatggctccg agtacaagat gtgcgtcttc tcgggccccg aacgtgatgc cgtcaccgcg
      841 ttcatccatg agagccttgc ggtgccggga tgcctgaccg gtggagtctc tgtccgtgca
      901 agtgacacgc agctcggccc ccacctgggg ccttctgatt ggatcacaag ctggtccctg
      961 ccaaccggcg atagcacaag actggtgttg ttccttgtag gcgagcgcct gaatgaagat
     1021 cactcaggca tcctgtcgac ctttctagcg acagcctctc tggctctgag gaacgcaaac
     1081 ttggccgacg gtctcagcaa gcggagcaac gaactcgtgt ccatcgtcta ctcgatgacc
     1141 aacccggtga tcgttgccga tgcccgaggg aacctcgtca cctcaaaccc ggcagccgag
     1201 gaactcttcg gattctcaac cgttttccag gtgggccacc acatcaacaa cgtactcaac
     1261 gacccgtccc tcgtctcgct cttgaccgag gatgttgacg acaccgtcga gaccgttgct
     1321 ggggtgacag agcgtcgagt gtggaaagcg aaatcctctc gtatccgctc gggacattct
     1381 cagctatctg gaagagtcct ggttctcgaa gatgtgaccg cggagcgcga gttcgctgag
     1441 atgaaggcga acttcgttgc ggtggtaggc catgagctcc gcacacctat gacgctcatc
     1501 aaaggcttct tgcggaccct tgttcgcaaa ggagacctca tgaccgaggc gcaacgaggc
     1561 gaaagcctgc ggaccctgga atcccaagct gtccggctcg aaagactcat cgaagacctt
     1621 ctattcgtca gccggatcga gaactctagg cccgagttgt tcgccgaaca cggcaacctg
     1681 atgcatgtga tccacgacct cttactcgag ttccagacac gggaacctgg caggcacctc
     1741 tcgatccagg ggcctgatcg cctggaccta gtcttcgata agaccagggt tgagcagatc
     1801 ctggttcatc tgctggacaa tgcatgcaag tattcggacc cctcaacacc tgtggtggtg
     1861 gcggttcacg aacatctcga tcacgtatcg gtggccgtca cggatagggg tatgggcatc
     1921 ctgtcgggag acctggaaga tctgttccgc cttctgcatc aggtcgatgg aacgagcacg
     1981 agagcggctg gcggcctcgg cgtcgggctg tacatatgca agagcctcgt tgaatcacac
     2041 ggcggtcaca tggaagtgaa ctcgctctgg ggaaagggtt cgaccttcac gttcactatc
     2101 cctaagggga tcgaagtagc gtctcgggcc atcacagcga cgcttaccga ccctcaggca
     2161 taagagttca ggaccttctg cgcaagtgcc ctgacgcgtt ccgccaccga aaccggctcc
     2221 acattgcgaa ctcgggggcc gagcatcaag agcatgcgct ccatccacac cgtatctgca
     2281 ccatcgacag aaactgaggt ccagccatcc tcggtctcac caacaacctt cgcacgtagt
     2341 ttctcgatca cccaactcga ctcaggagat acttccagcg tgagctcctg cccaggagga
     2401 ccctcgaagt cgatcccgct gtaacgcgtc gcatcttgct gtgaggggac gtagatctct
     2461 tcgaggagtt ccagacttcg gatccggtcc accctgaaga cgcgatccgc ctgggctcta
     2521 tgacagtagg cccagacgta ccagattccc tcccagacga atagtcggtg ggggtcaacc
     2581 ttgcgctctg tgatctcctc cttggatcca gattggtaga tcaggtggat ccggcggtga
     2641 tcatgcatgg cgccacggat cacagccacc tcctcgagct gatccacatc aacagccagg
     2701 cgaccagcaa catgctcacc catggcaccg gccaatttct ccatggctga gcgaaggttc
     2761 ttgtctgcat cccctgttgc tgcgagagcc tggactccgg cgaagagcat gatcgcctcc
     2821 gatggggcga gattgagcgg tcgggcgaag tagtctgccg accggatcga caccaggtct
     2881 ccttcgagga ccacctcgat caggtcaccc ggcgaatatc ctgggagccc gcacatctga
     2941 atcatgtcca ggtcgttcat cagctgccgc ttgttcactt gaaagatatc gaccagctcc
     3001 tgcacttcga tcgccccccg ctcttggacg aggttgatca tggcgatcag gcgtcggaac
     3061 ttatcgtgag tagactcagc caagacggct ctccagttca gaacgcactt cgtcgatcag
     3121 gtgctgcgga gacagaagat gtgcatcttc tccgaaccag aagagccatc tgatgaagcc
     3181 gttaacgttc gtcactgtga ggtcggcttc ggcagaaccg tcgggatggt cctcgagtct
     3241 cagccaggga gttgtcctgc cgacccacca ggctagggtg ggctcgaatc tcactcgagc
     3301 cacggcgaac acatcgtcac ccaccacgaa aggtggtgag tccaggacgt gactaaggtc
     3361 gaggtcgtcg ggtatcgaga aggagggttc gccgccggga tccatcacga gcatggtgcc
     3421 gcggatcctg tcgagccgga aggctctggt cgcatcacgg tcgtggtccc ggccgatcag
     3481 ataccaggcc ccccgtcgat tcttcagatt gtagggatcc agagtgcgtc tctcagcgtc
     3541 ggatcccttg cggtattgaa aggtaaccga tctgcgacgc gagacggcct cgaaggctat
     3601 ggtgaggttc ggatcagaga gacctacatc agccgataga cccgacgact catgaccttc
     3661 ggggactcct ccggccagtt tcagcagcgc attcctagcg acactgtcgt tctcacgagc
     3721 gaccatggtg acggccaacc agagagccgc cgcttctgct gatgtgagtt cgacatcggg
     3781 gaggtagaag tcctccttta cgatgcggta tcccttctcg acttcccaga tgtctgtcgg
     3841 agcaaagtca acctggatgc ccatctctcg aagatcctgc ttgtcgcgct ccagcatcct
     3901 gcggaatgtg tcgtcattct cctggtcata tccgggaatc gtggtcttga tctccgctga
     3961 agtgactggc gcgcgcgctg ccaggagcat gatcagcaga ttcatgacgc gctctgcctt
     4021 cttggtgctc actattcgta gcttccaaga accatctgac ctgcaaccaa acctgcagcc
     4081 ccggcggcct tgaagaaggc cggatcgtcc tttgcgctgc ggcccatggt agtgactctc
     4141 aagtcgagat ccgaaagaac ggtcagcgtt tcccaggcat cgacgatcct cacatcatgc
     4201 atctgggcga cgccggtctc gatcaggtcc tccatcactg cgctcagttc tgcgacatcc
     4261 aggtcgggaa cggcaacggt gcagccggcc gtggtgccga accgaagcgc gccgatcgtg
     4321 tgatggctca cacccctgtg tcgctcacgg gcgtcggcga acgagacgcg cagcgccgcc
     4381 acg
//
LOCUS       DATDOZ010000055         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_564666, whole genome shotgun sequence.
ACCESSION   DATDOZ010000055 DATDOZ010000000
VERSION     DATDOZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564666"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..893
                     /locus_tag="VI541_01060"
     CDS             147..893
                     /locus_tag="VI541_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69525.1"
                     /translation="MDPRRSLKGRGEWVFLALGIFVFGAGLAGNLVGGSRPAKPLSEH
                     DVHATPNVSVGPGEGVLIDAYIRTRRGALSELETQRPNATARAVVSFDRYLTGLEVER
                     LLDQHGLRALRLLTRIPLTGFKDRSIVVGPEGISQALITLTGTETQSKIRDELEELIK
                     LIPTVDQPEFKRVYEEDAANHRRALEMLDGDPAIIYAVNVEGTAADLVGIAGSAAVRL
                     VDLVPHGAPANVSLFGLHPEDETKTGSGPR"
     gene            942..1757
                     /locus_tag="VI541_01065"
     CDS             942..1757
                     /locus_tag="VI541_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69526.1"
                     /translation="MALLSACQPKDVPDKKASLSRSPSPSAGAQVSGTSTPSAAPSAT
                     SSILPPPEVKGGSLSRSAVQPPKPGDYLMDATGKIKISGCMDTERPPPTPMTVTVEAP
                     DGDRQRITQSSGGGSGDGQRTTLVLEYRVEGVYLTFLRIERTGIASSASDFTPNPPVL
                     VYPADPRVGQSWTYTLATGDGSMKIDSTSTVSGLSESVQLGDGSRASAHRITTARHVT
                     AKTTQGDTDVRSTEVSWFAPAPRLQVRSVEDSSGTSGICRLDSHVESVLRSAG"
     gene            1891..>2541
                     /locus_tag="VI541_01070"
     CDS             1891..>2541
                     /locus_tag="VI541_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLF69527.1"
                     /translation="MDRIEVKSGAEVSFVHLHSHSEYSMLDGASRINRSEHSNQPTMF
                     DRAAELGMPALALTDHGVMYGAIPFYLEGRSTGVKPIIGMEAYVAPKSRFDRPAGRED
                     WYFHLTTIAATNAGYQNLIKLASLGFTEGYDSRSRRPRVDKELLGEYADGIVVLSGCL
                     SGELPKNIVNGRLDEAERIAAEYREIFGDRYFLEIQNQGLVQQAPLNAAIARIGMKL"
BASE COUNT          489 a          808 c          764 g          480 t
ORIGIN      
        1 gcccgccgga tcggcctgcg ccggatgttc ctgcacgcct ctcggctctc tttcctgcat
       61 ccgaccaccg gcgagcaggt tgcgatcgtc gaaccccttc cgccggatct cgaggatgcg
      121 gtatcgaaac ttcgcagcac acgatgatgg accccaggcg atccctgaaa ggaagaggcg
      181 agtgggtctt tcttgccctg gggatcttcg tcttcggggc cggtctggcc gggaacctgg
      241 tgggtggttc aagacccgcg aagccgctct cagagcacga cgtccatgcg actccgaacg
      301 tgagtgtcgg acccggcgaa ggggtgctga tcgatgccta tatcaggact cgacgcggcg
      361 ctctctccga gctcgaaacg cagcgaccta acgcgaccgc ccgggctgtg gtttcgttcg
      421 ataggtatct caccggcctc gaggtcgagc ggttactcga tcaacatggc ctccgtgcac
      481 ttcgacttct cacgcgcatc cccctcacag gcttcaagga ccgcagcatc gtcgtcggcc
      541 ccgaagggat atcgcaggcg ctgatcacac tcacaggaac agagacgcag agcaagatca
      601 gagacgaact cgaagagctg atcaagctca taccaactgt cgaccaaccc gagttcaagc
      661 gcgtctacga agaggatgct gccaaccatc gcagggccct cgagatgctc gatggtgatc
      721 cggcgatcat ctacgccgtc aatgtcgagg gtaccgctgc cgatctggtg gggatcgcgg
      781 gatccgcggc ggtacgactc gtagacctgg tccctcacgg tgctcccgcc aacgtgtcac
      841 tattcggtct tcatcccgag gatgaaacca aaaccggatc gggcccacgg tagatgacag
      901 agagaacttt cattaagcct cgagccgcga cgttggttgt cgtggcactt ctatctgcgt
      961 gccagcccaa ggatgtgcct gacaagaagg cgtcacttag tcgttcgccg agcccctccg
     1021 cgggcgcgca ggtctcgggc acctcaactc catcagccgc accctcggca acctcctcga
     1081 tcctcccgcc gcccgaggtg aaagggggat cgctctcgcg ctcggcggtg cagcccccca
     1141 agcctggtga ctatctgatg gatgcgactg gcaagatcaa gatctccggc tgcatggata
     1201 ccgagcggcc cccaccgaca ccgatgaccg tgaccgttga agcgcctgat ggggatcgac
     1261 aacggataac tcagtcgtcg ggcggcggca gcggtgacgg tcagcggacg acgctcgttc
     1321 tggagtacag ggtggagggc gtctacctga cctttcttcg gatcgagagg accggcatcg
     1381 ccagctcagc cagtgatttc acacccaacc ccccggtgct cgtctacccg gccgatccac
     1441 gggtagggca gtcatggacc tacacgctcg cgaccggtga cggctcgatg aagatcgact
     1501 caaccagcac cgtctctggc ctctcggagt cggtgcagct cggggacggc tctcgggcca
     1561 gcgctcatcg gatcaccacg gccaggcatg tgaccgcaaa gaccactcag ggggatacgg
     1621 atgtgcgcag caccgaggtc agctggttcg ccccggcccc ccgcctccag gtcagatctg
     1681 tcgaggactc ttcaggcacc tcagggatat gccgcctcga ctcgcatgta gagtcggtcc
     1741 tgcggtcggc tggctagccg actggacgca gactcgggct agaccccagg cggtcccggc
     1801 ggtagtatgc ttctagttac agcgctgtaa tttcttcccc actcgccttg cgcttggttt
     1861 cgttgcttat cgggagggca agatgggcgg ttggaccgca tcgaggtcaa gtcgggggca
     1921 gaagtgagct tcgtacatct gcattcgcac tccgagtatt cgatgctcga tggtgccagt
     1981 cgcatcaatc gatcagagca ttccaatcag ccaactatgt ttgatcgggc agccgaactg
     2041 ggcatgcccg cactcgcgct taccgaccac ggcgtcatgt acggggccat cccgttctac
     2101 ctcgagggaa ggtcgaccgg ggttaagccg atcatcggca tggaggccta tgtggctccc
     2161 aagagccgat tcgacagacc tgccgggcgg gaggactggt acttccacct caccaccatc
     2221 gccgcgacga acgctggcta tcagaacctc atcaagctcg cgtcactcgg cttcaccgag
     2281 ggttacgact cacgctcgcg caggccgcgg gtcgacaagg agctgcttgg cgagtatgca
     2341 gatggcatag tggtgctctc aggctgcctt tcgggtgagc ttcccaagaa catcgtcaat
     2401 ggtcgcctcg atgaggccga acggatcgcc gccgagtatc gcgagatctt cggcgatcgc
     2461 tacttccttg agatccagaa tcagggtctt gttcagcagg cccccctgaa cgcggcgata
     2521 gcccgcatcg gtatgaagct a
//
LOCUS       DATDOZ010000056         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_565021, whole genome shotgun sequence.
ACCESSION   DATDOZ010000056 DATDOZ010000000
VERSION     DATDOZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565021"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..538)
                     /locus_tag="VI541_01075"
     tRNA            complement(466..538)
                     /locus_tag="VI541_01075"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(503..505),aa:Asn,seq:gtt)
     gene            complement(572..1012)
                     /locus_tag="VI541_01080"
     CDS             complement(572..1012)
                     /locus_tag="VI541_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69528.1"
                     /translation="MPGRRVAWFFLTVFLAGFFLACSPKPIITGKFGAGVASKIEKVP
                     VPSTAILDARPDPVVAYTIPNEDDLKGLAEWYNSRFPLNSDWERGWKWCAGAQADAKG
                     EGFRRHYRRDGRLLSFSIGGARTINDQDVLSIAIAVAEDLGATC"
     gene            complement(1016..2278)
                     /gene="rpoD"
                     /locus_tag="VI541_01085"
     CDS             complement(1016..2278)
                     /gene="rpoD"
                     /locus_tag="VI541_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HLF69529.1"
                     /translation="MSSAVVSTYAEEVLMAKEAQIDDVKDLVARGKEKGWLTADEIAD
                     ALGGLELSAEQVDGVYSLIAEEGIEVVEHEPGAATEELEDFRKDTELLKAPTNDPVRM
                     YLKEIGKVPLLTAEQEVDLAMRIEAGLWAEEMIEVPAKFDVERIRSLAERSHLNLEMG
                     SGRLSQDKAREIARRVSLDGLLGKSKLVEANLRLVVSIAKRYVGRGMLFLDLIQEGNL
                     GLIRAVEKFDYTKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLLRIQR
                     QLMQDLNREPLPEEIAEQMEMPPEKVREIMKVSQEPVSLETPIGEEEDSHLGDFIEDS
                     DAVIPLDAASFILLQEQLESVLHTLSEREKKVIQLRFGLHDGTPRTLEEVGKEFGVTR
                     ERIRQIESKTLSKLRHPSRSQKLRDYLE"
     gene            complement(2247..>2948)
                     /locus_tag="VI541_01090"
     CDS             complement(2247..>2948)
                     /locus_tag="VI541_01090"
                     /inference="COORDINATES: protein motif:HMM:NF021895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69530.1"
                     /translation="GAEVTEKVLGEAEPLMRFVLERSLQGAALDTPGSKAKAVSATAA
                     ILAWEPNRVARSDYVFWLARRIGVDPLQIQQEVSEVQSDPNRAGPATRIERLPGHVKV
                     EREALALVIDSPAELKRVSTWVTGDHFTVAEHRVILNVLLEYSRSTEGTFMDGLPDDE
                     TRRLAAELALSQLTTQDPEEVFLRLEEFRLRRQIASLRTKLDRLDPDMDPDGYAELFA
                     ELMKLDEQRRRFDLR"
BASE COUNT          570 a          925 c          877 g          576 t
ORIGIN      
        1 agccccaccc cgctcccgcc aaccccgagc ccgagtccct cgcccccggg cccaacaccg
       61 agcccgaccg aatcacccac gtcccctggg ccataggccc tcctatcccc cggatggtgg
      121 atatctacca gtgggttttc agccgaaaac tacggataag aggggtctat cggacccccg
      181 cccatccaag aagtgaaggt ggggtcgatg gaggaagatc tgccggcggt ccgcacgatc
      241 agggcgacca tccgcgacct ccaatcgata gccgatgaat tgggggcccg gctaaccagg
      301 tcccagcgcg gcgcgatcgc ccacagcgtg gagctactgc gcgagcaggt cgagaaggag
      361 agggcccagg ccgtcgagat cctggaccgg gctagacgct cccccatcat cgtcatacca
      421 gaaacgccag aactggaggg cagcactatg gctcgaagct cgaagctcgg ggggagggac
      481 tcgaaccctc aacctactga ttaacagtca gccgcgctgc cggttgcgcc acccccgaat
      541 gaggcagaga cgaggatagc atcgagtcgc gtcagcaggt tgcgcccaga tcctccgcca
      601 ccgcgatcgc gatcgagagc acgtcctgat cgttgatcgt ccttgcgcct ccgatggaga
      661 acgagagtag gcgaccatcg cgccgatagt ggcgccggaa cccttctccc ttggcgtcag
      721 cttgggcccc ggcgcaccac ttccaccctc tctcccaatc agagttaagg ggaaagcggg
      781 agttgtacca ctcggcaaga cctttgaggt cgtcctcgtt ggggatcgtg taggccacaa
      841 cagggtcggg ccgagcgtcg aggatggcag tggaagggac cggcaccttc tcgatcttcg
      901 aggcaacacc agcgccgaac ttgcccgtga tgatgggttt gggtgaacag gccaggaaga
      961 agcctgccag gaagaccgtt aggaagaacc atgcaacgcg acgaccgggc atctgctact
     1021 cgaggtagtc tcgaagcttc tgagaacgcg atgggtgcct aagcttcgag agggtcttcg
     1081 actcgatctg gcgtatccgc tcacgagtga ccccgaactc tttgcccacc tcttcgagcg
     1141 ttcggggcgt tccatcgtgc aacccgaaac gcaactggat gaccttcttc tcgcgttcgg
     1201 agagcgtgtg gagcactgac tcgagctgtt cctgcagaag gatgaaactc gctgcatcga
     1261 gtgggatgac cgcatctgag tcctcgatga aatctcccag gtgagagtcc tcttcctcgc
     1321 cgatcggcgt ctctagtgag accggttcct gggagacctt catgatctcg cgcaccttct
     1381 cggggggcat ctccatctgc tcggcgatct cttctggaag aggctcgcgg ttgaggtcct
     1441 gcatgagctg tcgttggata cgaaggagct tattgatcgt ctcgaccatg tgaacaggga
     1501 tgcggatcgt tcttgcctga tccgcgatgg ctcgcgtgat ggcctgacgg atccaccatg
     1561 tggcataagt cgaaaacttg tagcccttgg tgtagtcgaa cttctcgacg gcccggatca
     1621 gaccgaggtt tccttcttgg atgagatcga ggaagagcat gccgcggccc acatatcgct
     1681 tcgcgatcga tacaaccaag cggaggttgg cctcgaccag tttgctcttg cccagcaggc
     1741 catcgagtga gacccggcga gcgatctcgc gcgccttgtc ctgcgagagg cgaccgctgc
     1801 ccatctcgag gttcaagtgc gagcgttcgg cgagagaacg gatacgctcg acatcgaact
     1861 tcgcagggac ctcgatcatc tcctcggccc acagacccgc ctcgatcctc atggcgaggt
     1921 cgacctcctg ctcggccgtg agcaaaggga ccttgccgat ctccttgaga tacatacgga
     1981 ccggatcgtt ggtgggggcc ttgagaagtt cggtgtcctt gcggaagtct tcgagctcct
     2041 cggttgcagc cccaggttcg tgctcgacaa cctcgatgcc ctcttcggcg atcaacgagt
     2101 agacgccatc gacttgctca gccgaaagtt caaggccccc gagcgcgtcg gcgatctcgt
     2161 cggcggtgag ccagcctttc tccttaccgc gcgcgacgag gtccttcacg tcgtcgatct
     2221 gtgcttcctt ggccatgagc acctcttcag cgtaggtcga aacgacggcg ctgctcatcg
     2281 agcttcatca gctctgcgaa caactcggcg tatccgtcag ggtccatatc ggggtcgagt
     2341 cgatcgagtt tagtccgtaa agaagctatt tgccttcgaa gacgaaactc ctccaaccta
     2401 aggaaaactt cttcgggatc ctgggtggtc aactggctca aagccaactc tgcggcgagc
     2461 cgccgagtct cgtcgtcggg gagcccatcc atgaatgttc cttcagtgga acgcgaatat
     2521 tccaagagaa cattgaggat cactcggtgt tcggccacgg tgaagtggtc gccggtcacc
     2581 caggttgaca cgcgcttgag ctcggccggg gagtcgatca ccagggcaag ggcctccctc
     2641 tctaccttga cgtgtcctgg gagcctctcg atcctcgtgg cgggccctgc ccggttcgga
     2701 tcggactgga cctcggagac ctcctgctgg atctggagag gatccacacc gatccttctg
     2761 gccagccaga agacatagtc actgcgcgcc acgcggttgg gctcccaagc caggatggcc
     2821 gccgtcgcag agaccgcttt cgctttgctg cccggcgtgt cgagggcggc cccctgcagc
     2881 gaccgctcga ggacgaatct catgagtggc tcggcttcgc ccagcacctt ctctgtcact
     2941 tccgcccc
//
LOCUS       DATDOZ010000057         3632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2397403, whole genome shotgun sequence.
ACCESSION   DATDOZ010000057 DATDOZ010000000
VERSION     DATDOZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397403"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VI541_01095"
     CDS             <1..139
                     /locus_tag="VI541_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905040.1"
                     /note="produces methionine from
                     2-keto-4-methylthiobutyrate and glutamine in vitro;
                     mutations do not affect methionine salvage in vivo
                     however; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HLF69531.1"
                     /translation="RAGVVVAPGSGYGAHGEGYVRFSLTLPDDRLAVGAERILRAMKD
                     R"
     gene            136..1464
                     /gene="hflX"
                     /locus_tag="VI541_01100"
     CDS             136..1464
                     /gene="hflX"
                     /locus_tag="VI541_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLF69532.1"
                     /translation="MTSEPTIKERVVLVGLARSRSEIEAAEESLDELAQLADTAGAEA
                     VGRVVQVRDNPDPATYLGKGKAREVVGQARMLKADAIIFDDELAPAQGRNLEDMAGVN
                     PLAEHAMKIIDRTGLILDVFSQHAQSSEGKLQVELAQISYMVPRLRGWGDVMSRIGGS
                     AGPGIGTRGPGETKLEVDRRRIQRRLVKLKKDLQDLERTRKLKRKGRIRAGVAQVSLV
                     GYTNAGKSSLLNALTGAGVLVEDKLFSTLDPTARRLEIPDGREVVLIDTVGFVRKLPH
                     QLVESFRSTLEETLVADLIIHVVDATRREPSEQIAAVESVLGEIKAELIPRLLVLNKS
                     DAIDSEHRERLAREFPDARFVSALTGAGTDELLAAVSERLRASRVFVSLRIPFDRGDL
                     VSALHDQAEIIDESHGESGTRLVARVPAEVLPRYLKYVVVEDPDASKSAD"
     gene            complement(1448..2602)
                     /locus_tag="VI541_01105"
     CDS             complement(1448..2602)
                     /locus_tag="VI541_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69533.1"
                     /translation="MLGLRSNHLVAGDIAGYLAPLSRTAREVEGPIARSSRSVPLTKQ
                     QLVLDLNGFPAGTDAFSGLKVDLTYRYRGLETDNVFRIPFVYSVKRIAGSWKVTSSVL
                     APDASLPLWATGAVKTSMTKHFLAIYRPGLERVDETLEIAEQARERLGKKLTIPLEQK
                     QLMLLARDRTDYLGTTTQLSPVSAIAQAETSYEVTERTIKVQSRQIVINLEQLYSNSL
                     PSETLQHELGHLALARVTRPFTPSWVSESAAMFLADTKPLNLWRTGTRTRRFASISFV
                     DLNRLAALGQHDVTGDSASFEYAYAAAAAWYLTETFGVPNYFEFYDSYSKVPAKSLFD
                     RLPHSGGAAANDQAILDLAVQTTARLLHASYGFEENELDRRVRTWISQQT"
     gene            complement(2697..3347)
                     /gene="lexA"
                     /locus_tag="VI541_01110"
     CDS             complement(2697..3347)
                     /gene="lexA"
                     /locus_tag="VI541_01110"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HLF69534.1"
                     /translation="MTTQDGSDLTERQRSVLDFIVHAVEEQGYPPTVRDIGEATGLNS
                     PSTVHAHLKTLTEKGYLRRDPTRPRAIEVLWEREVGSMESRPSVRNLPIYGAIAAGPT
                     SLAQQEHEGILPFPKEFIGDTPHFALTVKGDSMIDAGILDGDLIVIRSQTDAANGDIV
                     AAQITGETGESESTVKRFQRKGNQILLVPANQTMEPIPAPPDLHILGKVVAVLRRL"
BASE COUNT          686 a         1025 c         1190 g          731 t
ORIGIN      
        1 gcgggccgga gtcgtggtgg ctcccgggtc agggtacggc gcgcatggcg aaggttatgt
       61 gaggttctct ctcacgcttc ccgacgatcg tcttgcagtg ggtgctgagc ggatcctgag
      121 ggcgatgaag gatcggtgac ctcagagcct acgatcaagg aacgtgtcgt cttggtgggt
      181 ctcgcgcgct cgcgctccga gatagaagcg gctgaagagt cgctcgatga gttggcgcaa
      241 ctggccgaca ctgcgggggc cgaggcagtt gggcgtgtcg tccaggttcg cgacaatccc
      301 gaccccgcga catacctcgg caaagggaag gcccgtgagg tcgttggaca ggcgcgcatg
      361 ctgaaagccg acgccatcat cttcgacgac gaactggccc ctgcacaggg acgcaacctc
      421 gaagacatgg ctggcgtgaa cccacttgcc gaacatgcga tgaagatcat cgatagaacg
      481 ggcctgatac tcgatgtctt ctcgcaacat gctcaatcgt ccgaggggaa actgcaggtc
      541 gaactggcgc agatcagtta catggtcccg cgccttcgcg gctggggcga tgtcatgagc
      601 cgcatcggag gaagcgccgg accgggcatc gggacccgtg gccccggaga gacgaagctc
      661 gaggtcgatc gtaggcggat ccaacgcaga ctcgtgaagc tgaagaagga cctgcaggac
      721 ctcgaacgca cgcggaaact caaacgaaag ggacgcatcc gcgctggcgt cgcgcaagtg
      781 agtctcgtcg ggtatacgaa cgcagggaag tcctcgctgc tcaatgccct gacgggggcc
      841 ggagttctcg tcgaggacaa actgttctca accctcgatc cgaccgcccg gcgactcgag
      901 atcccagacg gtcgcgaggt cgttctcatc gacacggtcg gcttcgttcg caaacttccc
      961 caccagctcg tcgagtcatt ccgatccact ctcgaagaga ccttggtcgc cgacttgata
     1021 atccatgtcg tcgacgcgac tcgaagggag ccgagcgaac agatagctgc ggtcgagtca
     1081 gtactcggcg agatcaaggc ggagttgata ccgcgactac tggtactcaa taagtccgac
     1141 gcgatcgact cagagcatcg tgagcggttg gcccgggagt ttcccgatgc tcgattcgtc
     1201 tcagcgctca cgggggccgg aacagacgaa ctgctcgctg ctgtttcgga gcgtctgcgc
     1261 gcatctcgag ttttcgtttc ccttcgcatc cctttcgacc gcggggacct ggtgagcgct
     1321 cttcatgatc aggccgagat catcgacgag tcgcatggcg agtccgggac gcgactcgtt
     1381 gcgcgtgttc cggcggaagt gctcccgaga tatctgaagt atgtcgtcgt cgaggatcct
     1441 gacgcctcta agtctgctga ctgatccagg tccgcacgcg tctgtcgagt tcgttctctt
     1501 cgaatccgta ggacgcgtgg agaagtcgcg ccgtggtctg aacggccaga tccaatatgg
     1561 cctgatcgtt cgccgcggcc cctcctgagt gcgggagtct gtcgaagaga ctcttagctg
     1621 ggaccttgga gtaggagtcg tagaactcga agtagttggg gaccccaaag gtctccgtca
     1681 ggtaccaggc tgccgcggcc gcatacgcgt actcgaatga tgcggagtcg ccggtgacat
     1741 catgctggcc gagggcagcg agccgattca ggtcgacgaa agagatcgat gcgaacctgc
     1801 gtgttcgggt tcccgtgcgc cagagattga ggggcttggt atcagcgaga aacatcgcgg
     1861 ccgactcgct cacccacgag ggagtgaacg ggcgggtcac gcgagccagg gcgagatgcc
     1921 ccaactcgtg ctgcagggtt tctgagggca ggctgttcga gtagagctgc tcgaggttga
     1981 tcacgatctg cctgctctga accttgatag tcctctcggt tacttcgtac gaggtctcgg
     2041 cttgagcgat ggccgaaacg ggggagagct gagtcgtggt gcccaggtag tccgtgcgat
     2101 cccgagcgag cagcatcagc tgcttctgct cgagcgggat cgtgagcttc ttacccagcc
     2161 gctctcgcgc ctgttctgcg atctcaagag tttcgtcgac ccgttcgagc cccggtcggt
     2221 atatcgcgag gaagtgtttc gtcatcgacg tcttcacggc ccccgtcgcc cagagcggaa
     2281 gggatgcatc gggcgcaagc accgaagatg tgaccttcca gctgccagct atacgcttga
     2341 cggagtagac gaatgggatc ctgaagacgt tgtcggtttc cagaccccgg taacggtagg
     2401 tgaggtcgac cttcagtccc gagaatgcgt ccgttccggc ggggaagcca ttgaggtcga
     2461 gaaccagttg ttgcttcgtg agagggactg agcgcgagct tctcgcgatg ggtccttcga
     2521 cctctctagc ggtgcgcgat agtggcgcga ggtaaccggc gatgtctcca gcgacaagat
     2581 ggttcgaacg caggccgagc atcgcgatga tctgctcctg cggtgatgga ggcggggtgc
     2641 gatgaaaggg ggagaggccg cacccctgta aaacaagtag gagtagagcg agcggtctag
     2701 agcctgcgaa gaaccgccac gaccttgccg aggatgtgaa ggtccggggg ggccgggatc
     2761 ggctccatcg tctggttggc tgggacgagc agtatctggt tgcccttgcg ctggaagcgc
     2821 ttgaccgtgg attcactctc tcctgtctct ccagtgatct gggcggcgac gatatctcca
     2881 ttggcagcgt cggtctgtga acggatgacg atgaggtctc catcaaggat accggcgtcg
     2941 atcatcgaat cgcccttaac cgtgagcgcg aagtgcggcg tgtcgccgat gaactccttg
     3001 gggaatggga gtatcccctc gtgctcttgc tgagcgagag aggttggtcc tgcggcgatc
     3061 gcaccgtaga tcggcaggtt tcgcaccgag gggcgtgatt ccatcgaccc aacctcgcgc
     3121 tcccagagga cctcgatcgc gcggggcctg gtcgggtcac ggcgcaggta ccccttctcg
     3181 gtgagggtct tgagatgggc gtggaccgtg ctgggggagt tcagtcccgt ggcctcgccg
     3241 atatccctga ctgtcggcgg gtacccctgc tcttcgacag cgtgaactat gaagtcgagg
     3301 accgaacgct ggcgctcggt caggtctgac ccatcctgtg tcgtcatctc tggcctccag
     3361 atctagatcc ccgcggggcg tctctaagta catatgttcg ggcgaggata gcagaaaagc
     3421 aaacgtatgt tcgatatttc gctcggaata gttgactcga acagatgttc gggactacga
     3481 tatcgacatg gagaacagac gttcgattca gctccgaaga gcggcagccc gactgggggc
     3541 cgtggccctt ctcatcggct tccttctggg gttgctggtg ccgcgcatct tcgcggccga
     3601 tgtttcacac tctggcgcgg tgcgtgttca tg
//
LOCUS       DATDOZ010000058         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1871386, whole genome shotgun sequence.
ACCESSION   DATDOZ010000058 DATDOZ010000000
VERSION     DATDOZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871386"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VI541_01115"
     CDS             complement(<1..903)
                     /locus_tag="VI541_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLF69535.1"
                     /translation="MFRTAKCSTSACYSAPVKDRSLIRAFSLIAHVDHGKSTLADRIL
                     QICKAVPEREMREQYLDKMDLERERGITIKAQAIRLSFPAADGKIYELNLIDTPGHVD
                     FSYEVSRSLAACDGVVLLVDAAQGIEAQTLANLYLALEAGLEIIPAINKIDLPAADPE
                     RVGKELSNLIGIDPSEIRRVSGKTGEGVEELIEQIVRKIPSPKGDPDATARALIFDST
                     YDNYRGVVTFVRVRDGELRARQKVKMMATGSVTEVEEVGVFAPTMTPVERLVAGEVGY
                     LIPGFKDVRQAHVGDTITDALRPAS"
     gene            919..1185
                     /gene="rpsT"
                     /locus_tag="VI541_01120"
     CDS             919..1185
                     /gene="rpsT"
                     /locus_tag="VI541_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLF69536.1"
                     /translation="MANIKSQKKRILTNEKRRVRNRNVSTALRTYVKRFNQAVEGGDS
                     DTARTALQFASRQLDKAASKGVIHANSAANRKSAMAKTLNSRTS"
     gene            complement(1211..>2061)
                     /gene="holA"
                     /locus_tag="VI541_01125"
     CDS             complement(1211..>2061)
                     /gene="holA"
                     /locus_tag="VI541_01125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HLF69537.1"
                     /translation="KVLEGLTNTFVDEFGPQEEPSVVAQALRTPSMFSDLRVVLIRDV
                     DTSVQEFQRALIDYALDPTPGSLLVMTASRSPAKLAAAVRKVGHVAEATRGRRPDLFA
                     WVKEKAREKALLLTADAGGALVEAVGEERAALAQAIETLSIAHGGRRVGPDEVREMFS
                     GHSDVKAFGFIDAVASRDVNTALESLHRLIRSGESAQGLFWMMVRHFRMLLLASGRST
                     STIAKDLGLPDWRAEKLVRQARGFSKARLIESYQELSKADRKMKRSEEPEVLTIERAV
                     VAIARD"
BASE COUNT          414 a          661 c          595 g          391 t
ORIGIN      
        1 cgaagccgga cgcagagcat cggtgatcgt gtcgcccacg tgggcttgac ggacatcctt
       61 gaaaccgggg atgaggtagc ccacctcgcc tgccacgagt ctctccacag gagtcatcgt
      121 gggtgcgaag acgcccacct cctcgacctc cgtcactgac ccggtcgcca tcatctttac
      181 cttctgacgg gcccgaagtt caccatctcg cacccgcacg aatgtcacga cacctcggta
      241 gttgtcgtag gtcgagtcga agatgagggc acgcgcggtc gcatctgggt cgcccttcgg
      301 tgaggggatc tttcgcacga tctgctcgat cagctcctcg accccctcgc ccgtcttccc
      361 tgagactcgg cggatctcgg atgggtcgat accgatgagg ttcgacagtt ccttccctac
      421 cctctccggg tcggcagccg gcagatctat cttgttgatg gcggggatga tctcgagacc
      481 cgcctcaagg gctagataga ggttggcgag cgtctgggcc tctatcccct gggcagcatc
      541 cacgagcagg acgactccgt cgcaagcagc aagtgagcgt gacacctcgt atgagaagtc
      601 gacatggccg ggcgtgtcga tcaggttcag ttcgtagatc ttgccatccg ccgcagggaa
      661 gctcaggcgg atcgcctgag ccttgatggt gatgcctcgc tcacgctcca gatccatctt
      721 gtcgagatac tgctcgcgca tctcgcgctc aggaaccgcc ttgcatatct gaaggatcct
      781 gtcggccagc gtggacttcc cgtggtccac atgtgcgatg aggctgaatg ccctgatcag
      841 ggagcgatct ttcactgggg cagagtaaca ggctgaggtg ctacacttcg ccgttcgaaa
      901 catacgacag gatcgaccat ggcaaacatc aaatctcaga agaagcgcat cctgaccaac
      961 gagaagcggc gcgttcgcaa tcgcaatgtc agcacggctc tgcggacata cgtgaagcgc
     1021 ttcaaccaag cggtagaggg tggcgactcc gatacagcac gtacggccct gcagttcgca
     1081 tctcgccaat tggacaaggc ggcatcgaag ggcgtcatcc acgccaacag cgcggccaac
     1141 cgcaagtcgg cgatggccaa gacgctgaac tctcgcactt cctaagcgtc aacggcggcc
     1201 ctgtcgctct tcagtccctc gcgatcgcga ccacagctcg ttcgatagtc aacacctccg
     1261 gttcttcgct tcgcttcatc ttgcggtcgg ccttcgatag ctcctgatat gactcgatca
     1321 gccgggcttt gctgaatccg cgggcctgac gcacgagctt ctcggcacgc caatcgggaa
     1381 gacccagatc cttcgcgatc gtcgatgtgg aacgtcccga agccagcaat agcatcctga
     1441 agtggcgaac catcatccag aagagaccct gagcggactc accagaacgg atcaagcggt
     1501 gaagagactc gagagcagtg ttgacgtcgc gtgaagctac ggcatcgatg aaaccgaatg
     1561 ccttgacgtc ggagtggcca ctgaacatct cacggacctc gtccgggccc acccttcggc
     1621 ccccatgcgc gatcgagagc gtctcgatgg cctgagcgag agccgccctc tcctcgccga
     1681 ccgcctcgac cagcgctccc ccagcatcgg cggttagcaa cagagccttc tcgcgggctt
     1741 tctctttcac ccaggcgaat aggtccgggc ggcgaccgcg cgttgcctcg gcgacatgtc
     1801 ccaccttgcg aaccgccgca gccagcttcg ctggtgatcg agacgcggtc atgaccagga
     1861 gcgaaccagg ggtcgggtcg agggcatagt cgatcagtgc tcgctgaaac tcctgcactg
     1921 aagtgtcgac atctcggatc aacacgaccc gaaggtccga gaacatcgac ggcgtgcgaa
     1981 gtgcctgcgc cacgaccgag ggctcctcct gggggccgaa ctcatccacg aaggtgttcg
     2041 tcagaccttc gaggaccttc t
//
LOCUS       DATDOZ010000059         5945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_51105, whole genome shotgun sequence.
ACCESSION   DATDOZ010000059 DATDOZ010000000
VERSION     DATDOZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51105"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VI541_01130"
     CDS             complement(<1..1421)
                     /locus_tag="VI541_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLF69538.1"
                     /translation="MSSSIDAWRGIAEETDEVSSDASIRLRRRSRQLLWSLLTPHKRA
                     LFLSTLLIILHTGAQLSLPYLVGRGIDRAIKPLAGAHGDPSTLWTIAVVLLGAVFTAS
                     AADRWFLSLTGKLGQDVLFDLRTRLFAHLQKLGIAFYERFTSGRVVARMTSDIDAISE
                     LLQTGLTSLAWAALSVVGIMVILLRLSLALGIVTLLSFPLILLLTHWFRRHSEQSYRA
                     VRNAVALVIVHFTESLRGVRAVHAYRREPRNQEIFEDLDAKYRDANIWSSWLAAILGP
                     GVNYLGRLTTLAIVVYGGFLLAHGKLTFGVLASFILYVRMFFDPLQELSQFYNVFQSS
                     AAALEKLSSVLEEVPTVPEKANAVATADIRGAIRFEGVSFGYTSESEVLHDVELDIPA
                     GQTVALVGETGAGKSTIARVISRFYDPTAGRITLDGHDLRDIALDLMHSSVVAVTQES
                     FLFSGTVSDNILFGRPSATREEV"
     gene            complement(1418..3178)
                     /locus_tag="VI541_01135"
     CDS             complement(1418..3178)
                     /locus_tag="VI541_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69539.1"
                     /translation="MQRSALRRVFSIYARPFAGRLVVVFGVATVALAASVSIPRVIKQ
                     AVDLVAGIHAGTIAPGELGKLWIYGAVLLGLASAEVVLAYIRRNTAARVSLQMETKLR
                     DDFYRHLQSLQISFHDNWQSGQLLSRAIYDINAIRRFVGFGAIFFGLSLIQLAGVFAM
                     LWSLDSQLAVLTFISVSPILWFSYRFGSGYRVVAKRIQDEQGDLSTLIEESASGIRII
                     KAFGRGALVLGNFSRQASKLRDSNLDAVKLRRTAWSTFGFFANLSLPVILLVGGFAVV
                     SGRLTIGGLVAFMAYLSMLIFPFDILGWILAMGEEARTAADRVFEVLDAKPEIQDRPG
                     ARALPHAQGRVAFEGIWFRYSDESDWILRDVNLEIEPGETLALVGKTGCGKTTLASLL
                     PRLYDATEGKVTLDDNDVRDLTLTSLRSHIGMAFEDPILFSASVTENILMGRPDATDD
                     DLRSALQTAQADFAHELPWGLDTRIGEQGYTLSGGQRQRLALARAVLGAPQVLVLDDP
                     LSAVDVHTEAMIEDALRTVLDGVTALLVVHRPSTLALADRVALLDNGTIVAIGKHHDL
                     MENVPLYRAILSQEAEEMQV"
     gene            complement(3343..4263)
                     /locus_tag="VI541_01140"
     CDS             complement(3343..4263)
                     /locus_tag="VI541_01140"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HLF69540.1"
                     /translation="MPVIPTKFIWMDGELVPWDEARIHVLSHTLHYGSGVFEGIRAYE
                     TKRGPAVFRLTEHIQRLARSSKIYLIDLPFTVGSLIDAVKETISANELKSCYIRPLVY
                     HGYGEMGLNPTVAPVNVAIITWAWGAYLGDDSFTKGVRCQISSWRRYDHNTLPPAAKA
                     TGQYLNSGLAKVDALRSGYDEAIMLNPEGYIADATGENVFIIRDDVLLTPPLHAGPLG
                     GITRDSIMTIARDLGVSVFETDLVRTDLYTADEAFMTGTAAELVPIRSVDDRDLGEPG
                     PTTKKIQEVFFATVRGEVERYSSWNEYVNQ"
     gene            complement(4370..>5945)
                     /gene="serA"
                     /locus_tag="VI541_01145"
     CDS             complement(4370..>5945)
                     /gene="serA"
                     /locus_tag="VI541_01145"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLF69541.1"
                     /translation="LVAEALAPEGIERLRSEGLEVDVSVGISREELLGRIEGADAVII
                     RSGTRIDSELIERAVNLKVIGRAGIGLDNIDLDAATRRGVLVVNAPQSNIHSAAEHTL
                     ALLLSMARRIPAADASMRAGAWERDRFTGVELFGKTLGVLGLGRIGTLVAQRAAGFGM
                     RLVGYDPYVSQSRAAQLGIEMLETVEELCSVADFITIHLPKTSETEAILGTRQFEVMK
                     AGVRIVNASRGGIIDEAALLAALQDGRVAGAALDVYAKEPPTGSPLVQLEEVVVTPHL
                     AGSTEEAQTKAGVAIAEQVLLALRGEFAPYAVNVEGGAGYVEMLKPFIPLTEKLGRIL
                     AAIAGAPLTEVRFEFHGTVAEQDTHVLTLAGLKGLLSSPDENVTFVNAPILAKERGIE
                     THETRSAVSLDWVNLVRMRGTSSAGEVAVAGSLVGKRDEERIVQVFEYTIDMLPERFM
                     TFLLYKDVPGVIGKVGSVLGEADINIAGMQVSRENIGGEALMGLTVDSPIPKEVLEQI
                     VETIGAREGRFIDLGA"
BASE COUNT         1228 a         1859 c         1804 g         1054 t
ORIGIN      
        1 tcaacttcct ccctcgtcgc agatggacgc ccgaacagga tgttgtccga aacagtcccc
       61 gagaagagga aactctcctg cgtaaccgcc acgaccgatg agtgcatcag gtccaaagcg
      121 atgtcgcgca ggtcgtggcc gtcgagtgtt atccgacccg cagtcggatc gtagaaacga
      181 gagatgacac gagcgatcgt cgacttgccg gcccccgtct cgccaacgag agcaaccgtc
      241 tgaccggccg ggatgtcgag ttcgacatcg tggaggactt cgctctctga ggtgtaaccg
      301 aaactgacgc cctcgaacct tatcgcccct cggatgtctg ccgtcgcgac cgcgttggcc
      361 ttttcgggga ccgtcggcac ttcctcgaga acgctggaga gcttctctag cgcggcagct
      421 gacgactgga agacgttgta gaactggctc aactcctgaa gcgggtcgaa gaacatccga
      481 acgtagagga tgaacgaggc caagacaccg aatgtcaact tgccatgagc gagcaggaag
      541 cccccgtaca ccacgatggc cagcgtcgtg agccgcccga ggtagttgac gccagggccg
      601 aggatcgccg cgagccacga ggaccatatg ttggcatcgc ggtatttcgc atcgaggtcc
      661 tcgaagatct cctggttgcg tggctctctg cggtacgcat gtacggcccg cacgccgcga
      721 agagactcgg tgaagtgaac gatgacgagc gcgaccgcat tgcggaccgc acggtatgac
      781 tgctcggagt gtcgtctgaa ccagtgcgtg agaagcagga tcaacgggaa agacaggagc
      841 gtcacgatgc caagagccaa cgagagtcgc aggaggatga ccatgatccc caccaccgag
      901 agcgccgccc acgcaagact cgtgagccct gtctgaagca gctcagatat cgcatcgatg
      961 tcggatgtca tgcgcgccac aacgcggccc gatgtgaatc gctcgtagaa ggcgatgccg
     1021 agtttctgca gatgagcgaa cagccgcgtg cgaagatcga agagaacgtc ctgacccagt
     1081 ttccccgtta ggctcaggaa ccatctgtca gctgccgacg ccgtgaacac cgcgccgaga
     1141 aggaccacag cgatcgtcca aagcgtgctc gggtcgccat gggctcccgc cagcggcttg
     1201 atcgccctgt cgatgccgcg acccaccaga tagggcagcg acaactgggc ccccgtgtgc
     1261 aggatgatca gaagggtcga gaggaacaga gcgcgtttgt gcggcgtcaa cagcgaccag
     1321 agcagctgtc ggcttctgcg ccgcagtcgg atggacgcgt cggacgagac ctcgtcggtc
     1381 tcctctgcta tccctctcca ggcgtctatc gaactgctca tacctgcatc tcctcagcct
     1441 cttgcgacag gatcgcccgg tagagcggaa cgttctccat caaatcgtga tgcttcccga
     1501 tcgcgacgat ggtcccgttg tcgagcaaag ccacccgatc ggccagcgct aacgtcgagg
     1561 gtcggtgcac taccaacagt gcggtcacac cgtcgaggac ggttctcagg gcatcctcga
     1621 tcatcgcttc ggtgtgaaca tcaacggccg atagcgggtc gtcgagcaca agaacctggg
     1681 gggcccccaa gaccgctcga gccagtgcaa gtcgctgccg ctgtcctccc gaaagggtgt
     1741 agccctgctc gccgatgcgg gtgtcgagcc cccagggaag ctcgtgagca aagtcagctt
     1801 gagccgtctg aagagcggat cgcagatcgt cgtccgtggc atccggacgg cccatcagga
     1861 tgttctcggt gacgctcgcc gagaagagga tcgggtcctc gaatgccatg ccgatatgcg
     1921 atcgcagtga ggtcagcgtg agatcgcgca catcgttgtc gtcgagggtg accttgccct
     1981 ccgtcgcgtc gtacagccgc gggagcagag aagcaagggt ggtcttgccg catccggtct
     2041 tgccaacgag cgccagagtc tcgcccggtt cgatctcgag gttcacatca cgaaggatcc
     2101 agtcggactc atctgagtac ctgaaccaga tcccttcgaa cgcgaccctc ccctgagcat
     2161 gcggcagagc ccgcgcgccg gggcgatcct ggatctcggg tttcgcgtcg aggacctcga
     2221 agacccggtc ggccgcggtg cgtgcctctt cacccatcgc caggatccag ccgaggatgt
     2281 cgaacgggaa gatcagcatc gagaggtacg ccatgaaagc aaccaggccc ccgatcgtga
     2341 gacgcccgga caccacagcg aaccctccga cgagcaatat gaccggcagg ctgaggttcg
     2401 cgaagaaccc gaatgtcgac caggccgtcc gacgaagttt cacggcatcc aggttcgagt
     2461 cacgcagttt cgaagcctgg cgggagaagt tgccgagcac gagcgctcca cgaccgaacg
     2521 ccttgatgat ccggatcccc gaggcagact cctcgatcaa agtcgagagg tcaccttgct
     2581 cgtcctggat cctcttggcc acaacgcggt agcccgatcc gaaccgataa ctgaaccaga
     2641 ggatcggcga cacggagatg aacgtgagga ccgccaactg cgaatccaaa gaccagagca
     2701 tggcgaaaac ccccgctaac tggatgagac tgagtccaaa gaagatggcc ccgaagccga
     2761 cgaaccgccg gatcgcgttg atgtcgtaga tcgcccgtga gagcaactgc cccgactgcc
     2821 agttgtcatg gaaagagatc tgcaggctct gcaggtgtcg gtagaagtcg tcgcggagtt
     2881 tcgtctccat ctgcagactc acccgagcag ccgtgtttct gcgtatgtag gcaaggacaa
     2941 cctcggcgct ggccagacca agaagcaccg caccgtagat ccagagcttg cccagttcgc
     3001 ccggagcgat ggttcccgcg tggatccccg ccaccaggtc gacagcctgc ttgatgacgc
     3061 gcgggatcga gacgctcgcg gctagagcga ccgtggccac gccgaagacc accaccaggc
     3121 gaccggcgaa tgggcgtgca tagattgaga agacacgacg cagcgcggag cgttgcatca
     3181 cagaaccttt ctgatcacga gactgcgccg gaagcccttg ttggggacgg tcctagaacc
     3241 gttggaaaga cgacagcctg aaagagacta ggggtaagag acgcccgccg acccgaggaa
     3301 ggaaaaagta tagcgcgacg tttgtcgcgt ttcaacggcg acttactggt tcacgtactc
     3361 gttccatgag gagtagcgct ctacctcgcc cctcacggtt gcgaagaaga cctcctggat
     3421 cttcttggtc gtcggccccg gctcaccgag gtcgcggtca tcgaccgaac ggatcggcac
     3481 caactctgca gcggtgcctg tcatgaaggc ctcgtcggcc gtgtagaggt cggtccgcac
     3541 taggtcggtc tcaaagaccg agacacccag gtcccgggcg atggtcatga tcgaatcacg
     3601 cgtgatgccc cccaggggcc cagcgtggag cggcggcgtg agcaggacat cgtctctgat
     3661 gatgaaaacg ttctcgcccg tagcatcggc gatgtacccc tctgggttca gcatgatcgc
     3721 ctcgtcgtag ccgctgcgca gtgcatcgac cttggcgagg cccgagttga ggtactggcc
     3781 cgtggccttg gccgcggggg gcagggtgtt gtggtcgtag cgccgccatg acgagatctg
     3841 gcaccgcaca cctttggtga agctgtcgtc gccgaggtag gccccccacg cccaggtgat
     3901 aatcgccaca ttgacggggg cgactgtggg gttcagcccc atctcgccat agccgtggta
     3961 gacgagagga cggatgtagc aggacttcaa ctcgttagcg ctgatggtct ccttcaccgc
     4021 atcgatcaga gatccaactg tgaatggtag gtcgatgagg tagatcttcg aagatctggc
     4081 caatcgctgg atgtgctccg tcaatcggaa gacagccggc cctcgcttgg tctcatacgc
     4141 acggatcccc tcgaagacac ccgacccgta gtgcagggtg tggctcagaa catggatccg
     4201 cgcctcgtcc cagggtacga gctcaccatc catccagatg aatttggtag ggatcaccgg
     4261 catgagtcag cctcctgaag gttcgtttgg aaaagcaccc gaagcctatt gtaatgggag
     4321 ctggggggcg tcggactccc ggcccgcgtt cgggcccggc aagcgaggct caggccccca
     4381 gatcgatgaa cctgccctcg cgtgccccga tagtctcgac gatctgttcg aggacctctt
     4441 tggggatggg cgagtccaca gtgagaccca tgagggcctc gcccccgatg ttctcgcgac
     4501 tcacctgcat gccggcgatg ttgatgtcgg cctcgccgag aaccgagccg accttgccga
     4561 tgactccagg tacatcctta tagagcagga atgtcatgaa tctctcggga agcatgtcga
     4621 tcgtgtattc gaagacctgg actatccgtt cctcgtcgcg cttgcctacc agcgaccccg
     4681 cgaccgcaac ctcaccagcc gacgacgtcc ccctcatccg cacgagattc acccaatcca
     4741 aagacaccgc agacctcgtc tcgtgcgtct cgatacctcg ctccttggcg aggatcggag
     4801 cgttcacgaa cgtgacgttc tcatccggcg acgagagcag gcccttcagc cccgctagcg
     4861 tgagaacgtg agtgtcctgc tcagcaaccg tgccgtggaa ctcgaaacgc acttcggtga
     4921 gaggtgcccc cgcgatcgcc gcgagtatcc gacctagttt ctcggtaagg gggatgaaag
     4981 gcttcagcat ctctacatag ccggcgccac cctcgacgtt gacggcataa ggagcgaact
     5041 cacccctcag agcgagcagg acctgctctg cgatggccac acctgccttg gtctgagctt
     5101 cttccgtcga gcccgcgaga tgcggcgtaa cgacgacctc ctccaactgg acaagcgggc
     5161 tgccagtcgg aggctccttc gcgtaaacat cgagagccgc ccccgcgacc ctgccatctt
     5221 gaagggccgc caggagagcc gcttcatcga tgatcccgcc gcgggatgcg ttgactatgc
     5281 gtacccccgc cttcatcact tcgaattggc gggtgcctaa gatggcctct gtctccgagg
     5341 tcttcggcag gtggatagtg atgaagtcgg cgacactgca gagttcctcg acggtttcaa
     5401 gcatctcgat cccgagttgg gctgcacggg actgcgagac ataagggtca tagccgacga
     5461 gcctcatccc gaaccctgct gcacgttgag cgacgagtgt tcctatcctc ccgagaccca
     5521 agacccccaa cgtcttgccg aagagctcaa cgcccgtgaa tctgtctcgc tcccaggccc
     5581 ccgcacgcat cgaggcgtcc gccgcgggga tccttcgagc catcgagagc aggagggcga
     5641 gggtatgttc agctgccgag tggatgttcg actgcggagc gttcacgacc agaactccac
     5701 ggcgcgtggc tgcgtcaagg tcgatgttgt ccaacccgat gcccgcgcga ccgatgacct
     5761 tcagattcac ggcgcgttct atcaactcgg agtcgatacg ggtgcccgag cggatgatca
     5821 cggcgtcggc accctcgatc ctgcccagaa gttcttcacg ggagatgccc accgagacgt
     5881 cgacttcgag accctcggaa cgcagccgct cgatgccttc gggggcgagg gcctcggcga
     5941 cgagt
//
LOCUS       DATDOZ010000060         3673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2148982, whole genome shotgun sequence.
ACCESSION   DATDOZ010000060 DATDOZ010000000
VERSION     DATDOZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148982"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3673)
                     /locus_tag="VI541_01150"
     CDS             complement(<1..>3673)
                     /locus_tag="VI541_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HLF69542.1"
                     /translation="RERSVKQMINRVVDWYSERGLGDGYFSTSEGVATFRDELSFLLV
                     RQFMAFNSPVWFNVGLVEPPRVSACFILDVEDEMSSILNWYVEEGLIFKAGSGAGVNL
                     SKLRSSREHLKSGGTASGPVSFMRGADASAGTIKSGGSTRRAAKMVVLDVDHPDVEEF
                     INCKVKEERKARVLREAGFEMDLDGADSYSLQYQNANNSVGLTDEFIQAYLGNRDWDL
                     KAVGDGSVLKSVKARDLMRQIAQAAWECADPGVFYLTTMNRWHTSPSEGPIRATNPCF
                     TGDTRVHTDKGLVRFDDLVIRAARGETFGIYTHDATNKDAPAECVELTNPTQYMVTGV
                     NEVLRLKFSNGMEVRATPNHKFFTTNRGMVPAAELTEADKVKVLNVATPAAFADWALP
                     LAALEATSRKKGEQRQNMNLPEKWSEELADYLGWLIGDGCFSNGGFSTIYGSKEDQSE
                     ILPRHQRFLKQITGGREPKVSVQENGTQQLRVTRHALIRFIEALGLKRATAPDKDVPS
                     SIFEAPQEIQAAFLRSLFDADGCVSLSQKEKGSRYVGLYSRSEALLQNVQRLLSGFGV
                     RSRIYAEKRVSPRKGFTYTRKDGTHVEYSSETGFDLRIFGRSIREFHERIGFSLSNKS
                     SKLQEAIGHEFYSVDESARLVSKESDGYELTYNLTEPRNHSYVVGGLVTANCGEYVRP
                     ANTSCNLASLRLTKFLDADGSFNVRDFISAIETTLTAMDISVTNAEYPTEKITEMVAN
                     YRELGLGYTDLGALLMDQGIAYDSDAGRAWAAATTALMSGVAYRRSAEIASVLGPYPG
                     WHKAGNSTAHLDVIRKHRAALDEIDGSLVPEDIVSAARKAWDDALEMGEKYGYRNAQV
                     TLIAPTGTISFLMDADTTGIEPDLALVKTKKLVGGGSMKIVNQRVPSALKRLGYLPNE
                     VSEIVSYISENNTVKGAPFMKPEHHSVFDCAMGESPIHYMGHVKMMSAVQPFLSGAIS
                     KTVNMPEDATVDDIESLYVEGWHLGLKNLAIYRDNCKVGQPLSADRKSDKVVVAGHEA
                     KAPVTAEGPVRRRLPRKRNSRTMSFRIADCDGYVMAGEYPDTEELGEIFLKVAKQGST
                     LSGIMDAFAISVSLGLQYGVPLKAYVEKFVNMRFEPSGVTDDPDFRFASSIVDYIFRR
                     LGADYLSEDDREANNIKTTLERQEDLDGGLPIVANGHSKSPDTELTLTQRPISDAPFC
                     YTCGISMRRAGSCFACESCG"
BASE COUNT          714 a         1086 c         1071 g          802 t
ORIGIN      
        1 ccgcatgact cgcatgcgaa gcacgaaccg gcacgtcgca tcgatatccc gcaggtgtag
       61 cagaagggtg cgtcagagat ggggcgctgg gtcagggtca gttcggtgtc agggctcttc
      121 gagtgaccat tcgccacgat cggcagccca ccgtcgaggt cctcctgcct ctctagtgtg
      181 gtcttgatgt tgttggcctc acggtcgtcc tcgctgaggt agtcggcccc cagtcgcctg
      241 aagatgtagt cgacgatgga gcttgcgaac ctgaagtcgg gatcatcggt gactcccgat
      301 ggctcgaagc gcatgttcac gaacttctcg acgtaagcct taagaggaac gccatactgt
      361 aggccgagtg agacgctgat cgcgaatgcg tccatgatcc ccgacagggt gctcccctgt
      421 ttcgcaacct tcaggaagat ctcgccgagt tcctcggtat cggggtactc ccccgccatg
      481 acatagccat cgcagtcggc gatccgaaag gacatcgttc gagagttcct cttgcgtgga
      541 agtctgcggc ggacggggcc ctcagccgtc acgggggcct tggcttcgtg cccagcaacg
      601 actaccttgt cgctcttgcg gtcggcggag agtggctgcc caaccttgca gttgtcccga
      661 tatatggcga ggttcttcag ccccagatgc cacccctcga catagaggct ctcgatgtcg
      721 tcgaccgtcg cgtcctcggg catgttcacg gtcttggaga ttgcccccga gaggaacggc
      781 tgaactgccg acatcatctt cacgtggccc atgtagtgga tggggctttc gcccatggcg
      841 cagtcgaaca cggagtggtg ttcaggtttc atgaagggag cccccttcac ggtgttgttt
      901 tcgctgatgt agctgacgat ctcggatacc tcattcggaa ggtagccgag acgtttcaga
      961 gcactcggga ctcgctggtt cacgatcttc atcgatccgc caccgaccag tttcttggtc
     1021 ttgaccagtg cgagatcggg ctcgataccg gtggtgtcag catccatgag gaaagagatg
     1081 gtcccggtcg gtgcgatcag agtgacctga gcgtttcggt agccatactt ctcgcccatc
     1141 tcgagagcgt catcccaggc cttgcgggcg gcagagacga tgtcctcggg aaccagggaa
     1201 ccgtcgatct cgtcgagtgc tgcccgatgt ttgcggatga cgtcgaggtg cgcagtcgag
     1261 ttaccagcct tgtgccagcc cgggtatgga ccaagcactg aagcgatctc ggctgaacga
     1321 cggtacgcga cgcctgacat gagcgcggtg gtggccgccg cccaggccct gcccgcgtcg
     1381 gagtcgtatg cgatcccctg gtccatcaga agggcaccga ggtcggtata tccaaggccc
     1441 agctcgcgat agttcgcgac catctcggtg atcttctcgg tcggatattc ggcattggtg
     1501 accgagatgt ccatcgcggt gagtgtcgtc tcgatcgcgg agatgaagtc tcggacattg
     1561 aagctgccgt cagcgtcgag gaacttggtg agacgaaggc tggccagatt acaagatgtg
     1621 ttcgcgggcc taacgtactc gccacagttc gctgtcacta gaccgccgac tacgtatgag
     1681 tggtttctcg gctcggtaag gttgtatgtc aactcatacc cgtctgactc cttggagaca
     1741 agacgtgccg actcatcgac tgagtagaac tcgtggccta tggcctcctg caacttgctc
     1801 gatttgttcg agagagagaa gccgatccgc tcgtggaatt cacgtatcga acgtccgaag
     1861 attcggaggt cgaaccctgt ctcagaggag tactcaacat gggttccatc cttgcgcgta
     1921 tatgtgaagc cctttcttgg acttaccctc ttctccgcgt agatacgtga gcgcaccccg
     1981 aaaccggaca gcaacctctg gacgttctga aggagggcct cggaccggct gtacagaccg
     2041 acatagcggg accccttctc cttctggctg agagacacac atccatctgc atcgaacagt
     2101 gatcgaagga atgcagcctg aatctcttgc ggggcctcga atatcgaaga cggaacatcc
     2161 ttatctggtg ctgtagcccg ctttaggccc aacgcttcga tgaatcggat gagagcgtgt
     2221 cgggtcacac gcagctgctg ggtcccattc tcctgcaccg aaaccttcgg ttcgcggccc
     2281 ccggtgatct gcttcaggaa ccgttggtgt cttggcagga tctctgactg gtcctccttc
     2341 gaaccataga tcgtagagaa ccctccgttg ctgaagcaac cgtcgccgat gagccatcca
     2401 agatagtcag cgagttcctc actccacttt tccggcaagt tcatgttttg gcgctgctcg
     2461 cccttcttgc gcgaggtcgc ctcgagagct gccaatggca gagcccaatc cgcaaaggca
     2521 gctggcgtcg caacattgag gaccttgacc ttgtctgcct ccgtcagttc ggcagcaggc
     2581 accatcccgc ggttcgtggt gaagaacttg tgattcggcg ttgcccttac ttccatcccg
     2641 ttagagaact tcagtcgcag gacctcgttc acgcccgtaa ccatgtactg cgtggggttg
     2701 gtcaactcca cgcattcggc aggagcatcc ttgttcgtcg catcgtgggt gtagatgcca
     2761 aacgtctcgc ctcttgccgc tcgaatgaca agatcatcaa atctgacaag tcccttgtcg
     2821 gtgtgaactc tcgtgtcgcc ggtgaagcag gggttagttg cgcggatcgg gccctcggat
     2881 ggcgaggtgt gccacctatt catcgtcgtg agatagaaga caccaggatc ggcgcactcc
     2941 caagcggcct gcgctatctg tcgcatcaag tcgcgagctt tcacgctctt gaggaccgaa
     3001 ccatcgccga cagccttcaa gtcccagtcg cgattcccca agtaagcctg gatgaactca
     3061 tcggtgagac ctacagagtt gttagcgttc tgatactgca aagagtacga gtcggccccg
     3121 tcgaggtcca tctcgaagcc ggcttcgcgc agcacacgcg ccttgcgctc ttccttgacc
     3181 ttgcagttga tgaactcctc gacatcgggg tgatcgacgt cgagcacgac catcttcgct
     3241 gcgcgtcgcg ttgaaccgcc cgacttgatc gtgccggcgg atgcgtccgc tccccgcatg
     3301 aacgagacgg gacccgatgc tgttcctccg gatttcagat gctcgcgtga cgagcgcagt
     3361 ttcgagaggt tgaccccggc ccccgagccc gccttgaaga tgagaccctc ctcgacgtac
     3421 cagttgagga tcgaggacat ctcgtcttcg acatcgagga taaagcaagc gctcactcga
     3481 gggggctcga cgagcccgac gttgaaccag accggagagt tgaaggccat gaactgtctg
     3541 accaagagaa atgagagttc gtcgcggaag gtcgccacgc cctcggaggt cgagaagtaa
     3601 ccatcgccca gtccgcgctc ggagtaccag tcgacgacac ggttgatcat ctgcttcacc
     3661 gagcgctcgc gct
//
LOCUS       DATDOZ010000061         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2659770, whole genome shotgun sequence.
ACCESSION   DATDOZ010000061 DATDOZ010000000
VERSION     DATDOZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659770"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1352
                     /gene="tuf"
                     /locus_tag="VI541_01155"
     CDS             162..1352
                     /gene="tuf"
                     /locus_tag="VI541_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLF69543.1"
                     /translation="MGKAKFERNKPHLNIGTIGHIDHGKTTLTAAITKTLTDRGLGNF
                     FTPFDQIDKAPEEKARGITITIAHVEYETEKRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMQQTREHILLARQVGVPSIVVAMNKVDMVDDPELLDLVELEVRELLT
                     FYEFPGDDIPVIRVSALKALEGDAAAQEQVMELMNAVDSGVPEPVRDVDKPFLMPIED
                     VFTITGRGTVVTGRVERGQVKVGEEIEIVGIHEKTQKTVCTGVEMFRKLLDSGQAGDN
                     IGALLRGIDKEAVERGQVLAAPGSITPHTDFEAQVYVLSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGAVSLGEGVEMVMPGDNTVMKVELIQPIAMDEGLQFAIREGGRTVGAGRVT
                     KILK"
     gene            1363..1524
                     /gene="rpmG"
                     /locus_tag="VI541_01160"
     CDS             1363..1524
                     /gene="rpmG"
                     /locus_tag="VI541_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLF69544.1"
                     /translation="MATDKRPHVILACGECKQRNYITTKNRSKQTERIELRKYCRWCR
                     THRPHKETR"
     gene            1524..2336
                     /locus_tag="VI541_01165"
     CDS             1524..2336
                     /locus_tag="VI541_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF69545.1"
                     /translation="MGRLDDRVAIVTGAGRGIGAATAKLFAEQGAAVVVNDVDKDPAQ
                     EVVEAITKAGGRALLNVDNTVNAEASQNLINATVEEFGKLDILVNNAGITRDKTFHNM
                     DDEQWDFVLDVNLRTAFNNSRAAVRHMRDAAKKELETTGAPAYHRKITFTSSTAGIQG
                     NAGQANYTTAKMGLVGLTRTLAVELGRFHINSNAVAPGFIETRLTQSKEQSTDPNLGI
                     PESLRGMALMMIPMGYYGQPEDVAKSHLFLSSSDSDYVTGQLIVVGGGLVRY"
BASE COUNT          526 a          746 c          700 g          387 t
ORIGIN      
        1 agcaagcggc tcataatcgc tgtgtcggcg gttcgaatcc gcccgccgct acagggtaaa
       61 gtttcccgtt cgcaccgact gctcatctat ggggccgtcg gcgagcgccg atatcagaga
      121 agaatacgcg tgaaggccca cgggccagaa ggagaaagag gatgggcaag gcgaagttcg
      181 aacgcaacaa accgcatctg aacatcggga ccatcggtca catcgaccat ggcaagacga
      241 ccctcaccgc agcgatcacc aagacgctga ccgacagagg actcggtaac ttcttcacgc
      301 ctttcgatca gatcgacaag gcacccgaag agaaggcccg cggcatcact atcaccatcg
      361 ctcacgtcga gtacgagaca gagaagcgcc actacgcgca cgtcgactgt ccgggtcacg
      421 ccgactacat caagaacatg atcacggggg ccgcccagat ggacggagcc atcctcgtcg
      481 tagcggccac cgatggtccg atgcagcaga cgcgtgagca catcctgctc gcacgtcagg
      541 tcggtgtccc ctcgatcgtc gtcgcgatga acaaggtcga catggtcgat gacccagaac
      601 ttctcgacct cgtcgagctc gaagtgcgcg agctgctcac cttctacgag ttcccaggtg
      661 acgacatccc ggtcatccgg gtatcggccc tcaaggccct cgaaggcgac gccgccgctc
      721 aggagcaggt catggagctc atgaacgcag tcgactcggg cgtccccgag ccggtgcgcg
      781 acgtcgacaa gcccttcctc atgccgatcg aggatgtctt caccatcacc ggccgtggca
      841 ccgtcgtgac cggacgtgtc gagcgcggac aggtcaaggt cggggaagag atcgagatcg
      901 tcggcatcca cgagaagact cagaagaccg tctgcacagg tgtcgagatg ttccgcaaac
      961 tcctcgactc aggtcaggct ggcgacaaca tcggcgctct cctgcgaggc atcgacaagg
     1021 aggccgtcga gcgcggtcag gtcttggcgg ctcccggatc gatcacgcct cacaccgatt
     1081 tcgaggcgca ggtctacgtc ctctccaagg acgaaggtgg ccgccacacg ccgttcttca
     1141 acaactaccg gccgcagttc tacttccgta ccaccgacgt gacgggcgcc gtatctctag
     1201 gcgagggcgt cgagatggtc atgcccggcg acaacaccgt catgaaggtc gagctcatcc
     1261 agcccatcgc gatggacgag gggctccagt tcgccatccg tgagggtgga cgcaccgtcg
     1321 gcgctggccg ggtcacgaag atcctcaagt aagggacgca agatggcaac cgacaaacga
     1381 ccgcacgtca tcctcgcttg tggtgagtgc aaacaacgca actacataac taccaagaac
     1441 cgttcgaagc agacggagcg catcgaactg cgcaagtact gcagatggtg tcggacgcac
     1501 agaccacaca aggagacacg ctagtgggaa ggctcgatga tcgcgtagcg atagtcacgg
     1561 gcgcggggcg cggcatcggt gcggccaccg cgaagctttt cgccgaacag ggtgcggccg
     1621 tcgtcgtcaa cgacgtcgat aaggatcctg ctcaggaggt ggtcgaggcc atcacgaagg
     1681 cggggggccg ggcgctcctc aatgtcgaca acaccgtcaa tgccgaggct tcgcagaacc
     1741 tcatcaacgc gaccgtcgag gagttcggca agctcgacat cctcgtcaac aatgcgggta
     1801 tcactcgcga caagaccttc cacaacatgg acgatgagca gtgggacttc gtcctcgacg
     1861 tcaaccttcg taccgctttc aataacagcc gcgcggctgt tcgccacatg cgcgatgcag
     1921 ccaagaagga gttggagacc acgggggccc cggcgtatca ccgcaagatc acatttacct
     1981 catcgactgc ggggatccag ggcaacgccg gtcaggcgaa ctacacgaca gcgaagatgg
     2041 gccttgtcgg actgactcgc acactcgcgg tcgagttggg ccgcttccac atcaactcca
     2101 acgcggtcgc gcccgggttc atcgagacac gcctgacaca gtcgaaggag cagtcgacgg
     2161 acccgaacct tgggatcccc gagtcacttc gaggcatggc cctgatgatg atcccgatgg
     2221 gctactacgg tcagcccgag gatgttgcca agtctcacct cttcttgtca tccagcgact
     2281 ccgactacgt caccggtcag ttgatcgtgg tcggcggggg cctcgtccgc tactaaccct
     2341 gaaggccgga cccttgaca
//
LOCUS       DATDOZ010000062         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1361171, whole genome shotgun sequence.
ACCESSION   DATDOZ010000062 DATDOZ010000000
VERSION     DATDOZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361171"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VI541_01170"
     CDS             <1..413
                     /locus_tag="VI541_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLF69546.1"
                     /translation="SLPIGRGRVVKQGDACTLITYGAMVARCLEVAEAASEDGVELEV
                     IDLRSLVPLDIDLIVESVSKTGRAVVVHEAPQTAGFGAEVVAQVAERAFLKLEAPILR
                     VTGYDTPYPPAKVEKHFVPSLDRILGAVERVLSF"
     gene            413..1651
                     /locus_tag="VI541_01175"
     CDS             413..1651
                     /locus_tag="VI541_01175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLF69547.1"
                     /translation="MAKVKKDFRLPDLGEGLEDAELVRWLVDLGDVVDLNQPIVEVDT
                     AKALVEIPSPVAGTIVTLHAEAGEVVKVGASLVTFEIEVSSDAEAEQPALLVGYGAQE
                     SRAPARRRRLSGARGPGKSGQADVASPPESAAEAGPPDAAPPVRKLASELGVDLDAIS
                     GTGPGGRITREDVLAASDATAGERDVERVPVRGARRLIAQKMARSVHEIPHVTTYLSV
                     DATEVLSLREQIIDSGVKVTPLAIVARAFVEVCKQNPLLNASFDAQAQEILLYRDCHL
                     GIATDTERGLMVPVVRDAHALRIAALGVQIAELTTKAREGRSEPSELLGGTVTISNVG
                     SFGAEYGTPIINYPESSILALGVIEARPVVRKGEVVVRPMATMSLSFDHRVLDGAQAG
                     RALMDLKILLEDGKRLAELR"
     gene            complement(1655..>2291)
                     /locus_tag="VI541_01180"
     CDS             complement(1655..>2291)
                     /locus_tag="VI541_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HLF69548.1"
                     /translation="TAFGVFQGLRAVSMELWNTADLAGRRLALQGVGKVGHALVGHLV
                     EAGAKVTIADIDVDNIARAVSDFGVETTDLDEIHKLDCDIFIPCALGGVLNDETIPEL
                     RCAAVAGAANNQLLSPEHGKLLEDRSILYAPDFIINAGGLINVADELRGYDPQRAMAQ
                     VEGIFHTLRKVFQIAKLEKITTAVAAEHLAEERIRQVGRIKLIQVGPRDVW"
BASE COUNT          410 a          681 c          752 g          448 t
ORIGIN      
        1 cctcacttcc gatagggagg gggcgcgtcg tgaagcaggg cgatgcctgc actctcatca
       61 catacggtgc gatggtcgcg cggtgcctag aagtcgctga ggctgcgtct gaagatggcg
      121 tcgaactcga ggtcatcgat ctgcgctctc tcgtgcctct agatatcgac cttatcgtcg
      181 agtcggtctc gaagaccggc cgtgccgtgg tcgtccacga ggccccccag acagcggggt
      241 tcggtgccga ggttgtggcg caggttgcag agcgggcctt cctgaagcta gaagctccta
      301 tccttcgggt tactggatac gacacaccct acccacctgc caaggtggag aagcatttcg
      361 tcccgagcct cgaccgtatc ctcggtgcgg tagagcgtgt gctctccttc tgatggcgaa
      421 ggtgaagaag gattttcgcc ttcccgatct gggtgagggg ctcgaggatg ccgaactcgt
      481 ccggtggttg gtggacctgg gtgatgtcgt cgatctcaac cagccgatcg ttgaggtgga
      541 caccgctaag gctctcgtgg agatcccatc tccagtcgcc ggcacgatcg tgacgctgca
      601 cgccgaggcg ggagaggtag tgaaggttgg ggcgagtctg gtcacattcg agatagaggt
      661 ttccagtgat gccgaggcgg agcagccagc tctgctcgtc ggttatgggg ctcaggagag
      721 ccgggccccc gctcggcgcc gacgattgag cggcgcccgg gggcccggca agagcgggca
      781 agccgacgtc gctagccctc ccgagtcggc agccgaggcc ggtccacccg atgccgcacc
      841 acctgtacgc aagctggcat ctgagctcgg tgtcgacctc gacgcgatct cagggaccgg
      901 tcctgggggc cggatcacac gtgaggatgt cctggcagcc tccgacgcga ctgcggggga
      961 gcgtgatgtc gagagggttc ccgtcagggg agcgagacgc ctgatcgctc agaagatggc
     1021 tcgttcggtg cacgagatcc cccatgtcac gacttatctg agcgttgatg cgaccgaggt
     1081 cctgtcgttg cgggagcaga tcatcgacag cggcgtcaag gtgacgcccc tcgctatcgt
     1141 cgcgagagcc ttcgtcgagg tgtgcaagca gaacccactg ctcaacgctt cgttcgacgc
     1201 ccaggcccaa gagatccttc tctatcgtga ctgccatctc gggatcgcca ccgacaccga
     1261 gcgcgggctg atggtcccgg ttgtacgcga cgcccacgca cttaggatcg ctgccctcgg
     1321 agtgcagatc gcggagctca cgaccaaggc gcgagaagga agatccgaac cgtcggagtt
     1381 gctgggcgga accgtgacga tctcgaacgt cgggtcgttc ggagcggagt acggcactcc
     1441 gatcatcaac taccctgaat cgtcgatcct cgcacttggg gttatcgaag cgcgcccagt
     1501 ggtccgcaag ggtgaggtcg tggttcgacc gatggcgacg atgtccctct ctttcgatca
     1561 ccgcgtcctt gatggcgcgc aggccggtcg cgctctgatg gacctaaaga ttcttctcga
     1621 agacgggaag cgtcttgccg aactgcgata ggagctacca gacgtcgcgg gggcccacct
     1681 ggatgagctt gatcctgccg acctgacgga tccgttcttc ggcgagatgt tccgcagcaa
     1741 ccgcggtggt gatcttctcg agcttggcga tctggaagac cttgcgaaga gtatgaaaga
     1801 tgccttcgac ctgagccatg gcccgttggg ggtcgtaacc ccgcagttcg tcagcaacat
     1861 tgatgaggcc cccggcgttg atgatgaagt cgggcgcgta gaggatgctg cggtcttcca
     1921 gcaacttccc gtgctccggc gagagcagct ggttgttcgc ggcccccgct accgctgcgc
     1981 accgtagttc cgggatggtc tcatcgttca gaacgccgcc aagggcgcag gggatgaaga
     2041 tgtcgcagtc gagtttatgg atctcatcga ggtcggtagt ctcgaccccg aagtcactga
     2101 cggctcgagc gatgttatct acgtcgatgt ctgcgatggt gaccttggca ccagcctcga
     2161 ccaggtgtcc gacgagtgcg tgtcccacct tgcctacacc ctgaagggct aaccgtcggc
     2221 ccgctaggtc tgcggtgttc cagagttcca tggacacggc acggagaccc tggaaaaccc
     2281 cgaaggccgt g
//
LOCUS       DATDOZ010000063         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3444399, whole genome shotgun sequence.
ACCESSION   DATDOZ010000063 DATDOZ010000000
VERSION     DATDOZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3444399"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="phoU"
                     /locus_tag="VI541_01185"
     CDS             complement(<1..654)
                     /gene="phoU"
                     /locus_tag="VI541_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HLF69549.1"
                     /translation="MTGTRRVFHHELRDLELRIQGMGAAVQEQVEHSVKGLASNDDGL
                     SRAVIEGDDDIDAYFIEIENKILDLFALQTPVASDLRLLTVLLHINLHLERVGDMAVN
                     VAKLSELSRDLPRNDNVLKHLEEMGAIDLKLIAAAMQALADRDLALCERLTVMDDPID
                     RLNRGMMDEVLAVSDDKRMLEWAIRMHVVSRQLERVGDHAVDIGEQVAFLITGEFREF
                     "
     gene            complement(655..1431)
                     /gene="pstB"
                     /locus_tag="VI541_01190"
     CDS             complement(655..1431)
                     /gene="pstB"
                     /locus_tag="VI541_01190"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HLF69550.1"
                     /translation="MSTGIEVAGLSAWYGDLIAIEDVTLSMAANSVTAIIGPSGCGKS
                     TLIRCLNRLHEVVPSSRVEGKVLINDTNIYEQGTDPVSVRRMVGMVFQRANPFPTMTI
                     YQNVAAGYRLNGRVAKKELDEVVERSLTHAGLLDEVKDRLDTRATSLSGGQQQRLCIA
                     RAIAIRPEVLLMDEPTAALDPAATLRIEDLIGELKKTFTVVIVTHNMQQAARVADNTA
                     FLLSERAGHAGKLIEYADTHKIFTRPDDPRTEDYITGRFG"
     gene            complement(1432..2286)
                     /gene="pstA"
                     /locus_tag="VI541_01195"
     CDS             complement(1432..2286)
                     /gene="pstA"
                     /locus_tag="VI541_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HLF69551.1"
                     /translation="MSVISGKRRATDIAARVLLATAVVITLIPLVWIIWSVLHLGIGA
                     IEGLSFFTEPPPGSVALSGGGVANGIIGTLMMVGIAALISIPLGVLGAIYLIEFSQDS
                     VLAKAIRFFAEVMTGIPSIVFGIFVYSVVVVATGGFSAMAGALALALIMWPIALRTTD
                     EVLRLVPSEVREAAYALGIPRWRTILKVVLPTAAGGIATAGMLAIARGAGETAPLLFT
                     ALGNQHIALGLGSPMSALPLEIFKGATGAFLAGNERAWAGALTLISLILLLSITARIF
                     AARRMRGA"
     gene            complement(2283..>2552)
                     /locus_tag="VI541_01200"
     CDS             complement(2283..>2552)
                     /locus_tag="VI541_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HLF69552.1"
                     /translation="GIFAAVMLGLGRALGETIAVTMVIGNSPKIAASIFSPGYTMASL
                     IANEFSEATGPSHLEALIAVGLLLFAVTLLINAGARLVIRKGLMS"
BASE COUNT          531 a          771 c          721 g          529 t
ORIGIN      
        1 gaactctcgg aactccccag tgatcaagaa cgcgacctgc tcgccgatgt ccactgcgtg
       61 gtcgccgacg cgttccagct ggcgtgacac aacatgcatg cgtatcgccc actcgagcat
      121 ccgcttatcg tccgagacgg ccagcacctc gtccatcatc ccgcggttga gacgatctat
      181 cggatcgtcc atgactgtga gcctttcgca aagagccagg tcgcgatcgg caagagcctg
      241 catggctgcc gcgatcagct tgagatcgat ggcccccatc tcctcaagat gtttcaacac
      301 gttgtcgttg cgaggcagat ctcttgaaag ctctgaaagc ttggcgacgt ttactgccat
      361 gtccccgact cgctcgagat gcaggttgat gtgcaagagc acggtgagga ggcgcaagtc
      421 ggatgccacg ggagtttgaa gcgcgaacag gtcgagtatc ttgttttcga tctcgatgaa
      481 gtatgcgtcg atgtcgtcgt ctccttcgat gactgcgcga ctcagaccat cgtcgttgga
      541 ggctaaaccc ttcactgagt gctcgacctg ttcctgtact gcagccccca tgccttggat
      601 gcgcaactca aggtcgcgta gctcgtggtg aaagaccctt cgtgttcctg tcatctaacc
      661 gaacctcccg gtgatgtagt cctcggtccg cgggtcatcg gggcgggtga atatcttatg
      721 cgtatcagcg tattcgatga gctttcctgc atgtcccgcg cgctcggaaa gcaggaatgc
      781 tgtgttgtct gcgacacgcg cggcttgttg catgttgtga gtaacgatga ccacggtgaa
      841 cgtcttcttg agttcaccga tcagatcttc gatccgaagg gtcgcggcgg ggtcgagcgc
      901 cgcggtgggc tcgtccatga gcaaaacctc cggccggatc gcgatcgccc ttgcgatgca
      961 aagacgttgt tgctgacctc ctgacaaact agtcgccctc gtatcgagtc ggtctttgac
     1021 ttcatctagg agccctgcgt gggtcaggct gcgctcgaca acttcgtcca attccttctt
     1081 ggcaaccctt ccattcagac ggtatccggc cgcgacgttc tggtagatgg tcatcgtcgg
     1141 gaatgggttc gcgcgctgga acaccatccc caccatccgt cggaccgata ccgggtctgt
     1201 gccctgctcg tagatgttgg tgtcgttgat cagcaccttg ccttcaacgc gtgagctcgg
     1261 cacaacttca tgcaaacggt tcaggcacct gatcagagtc gacttcccac agcccgaagg
     1321 cccgatgatc gcagtgacgg agttggcggc cattgagagc gtcacatctt cgatcgcgat
     1381 caggtcgccg taccaggctg aaagccccgc gacctctatc ccggtactca ttcaggcacc
     1441 cctcatgcgt ctggccgcga atatccgcgc agtgatagag agcagcaata tcaacgagat
     1501 gagagtcaag gctccagccc acgccctttc attgccagca aggaacgcac cggtggcacc
     1561 tttgaatatt tccagtggga gcgccgacat cggacttcca agaccaagcg cgatgtgctg
     1621 gttgcccagc gcggtgaata aaaggggagc ggtctcaccc gcgcccctgg cgatcgccag
     1681 catgccggct gtcgctatgc cgccggccgc tgtaggaaga accaccttca agatggtgcg
     1741 ccacctcggt atacccaacg cgtatgcggc ttccctcacc tcggacggca ccagccgcaa
     1801 gacctcgtcg gttgtcctta gcgctatcgg ccacatgatc aaggcaagag ctaaggcccc
     1861 cgccatcgcg gaaaaccccc cggtggccac cacgaccacg gagtaaacga atatcccgaa
     1921 gacgatcgat gggatcccgg tcatcacttc tgcgaagaag cggatcgcct tcgcaagcac
     1981 tgagtcctgg gaaaactcga tgagatagat cgcgcccagt actcccaatg ggatggatat
     2041 aagcgcggcg atgccgacca tcatcaaggt tccaatgatg ccgttggcaa ctccgcctcc
     2101 cgagagggcg actgatcccg gtggtggctc ggtaaagaag gacaagcctt cgatggcccc
     2161 gatcccgagg tgaagaacag accagattat ccaaacgagg gggatcaagg tgatgacaac
     2221 ggcggtggcc agcagtaccc gcgctgcgat gtctgtggcg cgccgcttcc ccgagatcac
     2281 gctcacgaca ttagtccttt ccttatcacc aatcgtgcgc cggcatttat caaaagtgtc
     2341 acggcgaaga gcagtaaccc gacggcgatc aaggcttcga ggtgggatgg acctgttgcc
     2401 tccgagaact cattggcgat gagcgatgcc atggtgtatc cgggggagaa tattgacgct
     2461 gcgatctttg gcgaattccc gatcaccatc gtgacggcga tcgtctcgcc caacgcacgt
     2521 cccaggccaa gcatcacggc tgcgaagatc cc
//
LOCUS       DATDOZ010000064         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_831025, whole genome shotgun sequence.
ACCESSION   DATDOZ010000064 DATDOZ010000000
VERSION     DATDOZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831025"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="tsaD"
                     /locus_tag="VI541_01205"
     CDS             complement(<1..811)
                     /gene="tsaD"
                     /locus_tag="VI541_01205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLF69553.1"
                     /translation="MIKGSLPLAEEVLEDFDVEEFETLPFLEEVPTPAREEILTLGIE
                     TSCDETSVAVLRGARDVLSNVISSSADLHGKFGGVVPEVASRAHVEAINPAIEQALRD
                     AQVTLSDISVIGVTIGPGLIGSLTVGVAAAKSLSSVLDIPLVGVNHLEAHLYACLLEH
                     KEADFPAFGLIVSGGHTLLVNAVGHGLYEIIGQTLDDAVGEAFDKIGRFLGLGFPGGP
                     AIDAAARRGDPKSVALPRAMRNEGYDFSFSGLKTAVVNHVKKQRAAGNEPKV"
     gene            complement(808..1353)
                     /gene="rimI"
                     /locus_tag="VI541_01210"
     CDS             complement(808..1353)
                     /gene="rimI"
                     /locus_tag="VI541_01210"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HLF69554.1"
                     /translation="MAIAKEQDFHPNDAELTRMRRRHVRAVMRIENLVYPRPWSSTLF
                     YQEIANRPDRVYLIARLEGEVIGYAGIMTSGYESHVTTIAVDPEYQGRRLGMKLMIGL
                     FDAAIENGGRTMSLEVRMTNAVAQTMYTRFGFKPVGIRRGYYVESGEDAIVMLCEQID
                     TVEFAQRLDDLREVVAGPLRS"
     gene            complement(1354..2052)
                     /gene="tsaB"
                     /locus_tag="VI541_01215"
     CDS             complement(1354..2052)
                     /gene="tsaB"
                     /locus_tag="VI541_01215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLF69555.1"
                     /translation="MLVLGIETSTPHSSVAIGSEQGIIASALVARGTSHGEFLLSAVE
                     FLLHQSDLRYRNISAVAVGVGPGLYTGLRIGITTAKTVAQALAVPIVGVSSLDVLAFD
                     VRHSSRSIWAVMDAKRNEIFFARYRQVPGGIARDGEPHLGTVETLIGELSAAREDSLL
                     VGSGALLNRARLEEAEKVEFSSLAESFPRSSSLVELALPRLFREDFDKVFEVAPLYMR
                     RSDAEIDWDRRNTT"
     gene            complement(2053..>2644)
                     /gene="tsaE"
                     /locus_tag="VI541_01220"
     CDS             complement(2053..>2644)
                     /gene="tsaE"
                     /locus_tag="VI541_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLF69556.1"
                     /translation="NSLLPPLLLLQKRVEMRSSPLSSDGSPDDSPEPVHIVTRCAEDT
                     RRLASAITPVLKPGDVIVLAGDLGAGKTTFVQGLGAGLGVNEPITSPTFILMKEYLGR
                     FPLLHLDVYRLSGVQELLDLGYDEFLAPHIVAVEWGDVVEPMLPRDHLKVELKAMADD
                     DERSVMLIGTGQTWDIRMKTMQTLISGLFDEPRGDA"
BASE COUNT          545 a          830 c          768 g          501 t
ORIGIN      
        1 cgaccttcgg ctcattccct gcagctcgct gcttcttgac gtggttcacg acggcggtct
       61 ttagacctga gaacgagaag tcgtagccct cgttgcgcat cgctcgagga agtgccaccg
      121 acttggggtc gccccgccgc gccgcggcat cgatagccgg cccccccggg aacccgagac
      181 ccaggaaccg accgatcttg tcgaatgctt ccccgaccgc gtcgtcgagg gtctgaccga
      241 tgatctcgta gagaccatgc cccacggcat tcaccagcag agtgtgtccg cccgagacga
      301 tgagtccgaa tgccgggaag tccgcctctt tgtgttccaa caaacatgcg tatagatgag
      361 cctctagatg gttcacgccg acaagcggga tatccagaac cgatgaaaga ctcttggccg
      421 cggccacgcc cacggtcagc gaccctatga gtccgggccc gatggtgaca ccgatcacgg
      481 atatgtcgct gagggtcacc tgggcatcgc gaagggcctg ttcgatcgcc gggttgatgg
      541 cctcgacgtg agcgcgtgat gcaacctcag gaaccactcc cccgaacttt ccgtgcaggt
      601 ccgctgatga agagatcacg ttcgaaagga catcgcgcgc tccccgaagg acggcaaccg
      661 atgtctcgtc gcaggaggtc tcgataccga gggtgagtat ctcctcacga gcgggagttg
      721 ggacctcctc taggaatggg agggtctcga actcttcgac gtcgaagtcc tctaggacct
      781 cctcggccag aggcagggag cccttgatca agatcgtagg ggacctgcga ccacctcgcg
      841 cagatcgtcc agcctctgag cgaactcgac ggtgtcgatc tgttcgcaga gcatcacgat
      901 cgcgtcctcg ccgctctcga cgtagtagcc gcgccggatg ccgacgggct tgaagccgaa
      961 tcgcgtatac atcgtctgcg ccaccgcatt ggtcattcgg acctccagcg acatcgtccg
     1021 gcccccattc tcgatcgcag catcgaagag accgatcatc agtttcatcc cgaggcgtcg
     1081 tccttgatat tcggggtcaa cggcgatagt tgtcacatgc gattcgtagc ccgatgtcat
     1141 gatccctgca tagccgatca cctcgccctc gagacgtgcg atcagataga ccctgtcggg
     1201 acgattcgct atctcttgat agaagagcgt cgaactccaa gggcgggggt agacgagatt
     1261 ctcgatcctc atcaccgcgc gcacatgtcg acgacgcatg cgggtcagtt cagcgtcgtt
     1321 cggatggaag tcctgctcct tcgcgatcgc catctaagtg gtgttccttc tgtcccagtc
     1381 gatctcagcg tctgaccgtc gcatatatag cggggcgacc tcgaatacct tgtcgaaatc
     1441 ttccctgaat agtcgcggaa gggccaactc gacgagtgaa gaggagcgcg ggaacgactc
     1501 tgccagagat gagaactcga ccttctcggc ctcctcgagg cgagcacggt tgagaagggc
     1561 cccactgccc accagcaggg agtcctcgcg agccgcggag agctcgccga tcagggtttc
     1621 gacggtaccg agatgcggct cgccatcgcg agctatgccg ccggggacct ggcggtatcg
     1681 ggcgaagaag atctcgttgc gcttcgcatc catgacggcc catatcgacc tcgatgagtg
     1741 ccgaacatcg aacgccagga catcgagcga cgagacaccc actatcggca ccgcgagcgc
     1801 ctgagccacc gtcttcgccg tcgtgatgcc gatcctcagc cccgtgtaca ggccgggccc
     1861 gactccgacg gccaccgccg agatgttgcg gtatcgaagg tccgactgat gcaatagaaa
     1921 ctccacagcg ctcagaagga actcaccgtg gctggttccc cttgcgacca gagcggacgc
     1981 gatgatcccc tgttcggagc cgatggcaac agaggagtgc ggcgtcgagg tctctatccc
     2041 tagcaccaac atctacgcgt ctcctcgcgg ctcgtcgaag agacccgaga tcagcgtctg
     2101 catggtcttc atcctgatat cccaagtctg accggttccg atcaacatca ccgagcgttc
     2161 atcgtcatcg gccatcgcct tgagctcaac cttcaagtga tcgcgaggca acatcggttc
     2221 cacgacatct ccccactcaa ccgcgacgat gtgcggagcg agaaactcgt catagccgag
     2281 atcgagcagt tcctgaacac cgctcaatcg atagacatcg aggtggagca gagggaagcg
     2341 ccctaagtat tccttcatca ggatgaaggt cggactggtg atcggctcgt tcacaccgag
     2401 tccggccccc aacccctgca cgaatgtcgt cttgcccgcc cccaggtcgc cagcaagcac
     2461 tatcacgtca ccgggtttca atacgggagt gatggcagag gcaagtcggc gagtgtcctc
     2521 ggcacatcta gtgacgatgt gcacgggctc gggcgagtcg tcaggcgatc cgtcagaaga
     2581 gagagggctg ctgcgcatct cgactctctt ctgtagtagg agcaacggag gaagcagaga
     2641 gttc
//
LOCUS       DATDOZ010000065         3771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1363998, whole genome shotgun sequence.
ACCESSION   DATDOZ010000065 DATDOZ010000000
VERSION     DATDOZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363998"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VI541_01225"
     CDS             complement(<1..631)
                     /locus_tag="VI541_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF69557.1"
                     /translation="MTEPIALVFVTTNEGHVLIPALDSLFSSVGETPLEVVVVDNAST
                     DGAGEQISRKWPNVRVITQDRPGGLPANLNRGIRETGSPYIMLCNSDLIFTPGAVETL
                     ARFMDEHPRCGIVGPLFNSPDGEPRVTARRWYTWPVLAALKGPWKPFTSRWAFVLHSF
                     YDEWNRQEPLEVDWVPCPGTMFRRAALDEVGLMDERFRLYFDDVDIALRM"
     gene            complement(628..1722)
                     /locus_tag="VI541_01230"
     CDS             complement(628..1722)
                     /locus_tag="VI541_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLF69558.1"
                     /translation="MNLLFLVPGEVGASEALLVNLVRSMSSSGHRIVVFGLRGLRGTY
                     PDLFDQVQLVEAPWSRGRQGLRIAAENTWLGWQARRWKLDVIHHGVGTTPLLKTVPSV
                     ATIHDIQYRHYPANFAAMKLRWLSFNVPMTLRSCRMVSVPSEWVKRDLVEQLGADAEK
                     VTVVPFGSEDPFAGPPADPDEARSRYRLDRPFLLYPARSYVHKNHRFLLEAYKPVSER
                     CDLVLTGAPWPRDRELADAARGLGLSGRVRQLGLIDRRDLGGLYLSACALVYPSRFEG
                     FGAPILEAMSVGCPVIASDATCIPEVVGEAGVLLDPDDLDGWTETISRVLEDDGYAAN
                     LATKGRERASGFNWERSARLQIETYERALA"
     gene            1758..2870
                     /locus_tag="VI541_01235"
     CDS             1758..2870
                     /locus_tag="VI541_01235"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLF69559.1"
                     /translation="MRLAFDVSAILGTRTGIGHYSTILLERLVSHDADIEIQPFALTT
                     KKDVSRIPESLRETLRRISVPARLGVTAWTRAGRPYAETYLDGVDVVHGPNFWVPPLS
                     SASGVVTIHDMTFSLYPEMCTPQVRRYAWIIPKMLNWTRLVITPSESVRSEVAEVMGF
                     PRDRIVVTPEGVRGSFVGVAPNQDAAREFGVDGPYVLFAGTQEPRKNLDRLIQAFAML
                     SDVEVQLLIVGPPGWGSVDLQAVATKLHVSSRVHFTGYVSDERLAVLMAGASAFAFPS
                     IYEGFGLPPLEAMAAGVPVVAGRAGSLEEILGEAPFWCDPLDTSSIADALSRCLHDDA
                     ARSRAKEAGRSRAGLYSWDETARLTIEVYERAQRIR"
     gene            2887..>3771
                     /locus_tag="VI541_01240"
     CDS             2887..>3771
                     /locus_tag="VI541_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HLF69560.1"
                     /translation="MPSPQRSLADLIKPGMVHPGRRTRRLGVRILIGLLLVAVLGVLA
                     TAGLYFLGGPKASHVKGLGELPAGEPMNVLVLGSDSREGLSKEELRKYDPQGTDRRSG
                     RRSDTIMLLHVDPDKEKVVMVSFPRDLRIRHPNGSLGRINAIYQQGPSAMVQAVKTYT
                     GLPIHHYVEVNFEGFKNIVDTVGGVDVFFERSIREPDSGLNVRKGCVHLVGDQALAFV
                     RVRKIDSDFGRIARQQLFLRLMMDKVTHAKILINPVKIVKLVEILSNNVTTDTGLSLG
                     DLKGLALQLRGFDQSRIDM"
BASE COUNT          725 a         1139 c         1155 g          752 t
ORIGIN      
        1 tcatccgcag agcgatgtcg acgtcgtcga agtagagcct gaatcgctcg tccatgaggc
       61 caacttcatc gagcgcagct ctgcggaaca tggttccggg gcagggaacc caatccactt
      121 caagaggttc ctggcggttc cactcgtcgt agaaactgtg cagcacgaac gcccagcgcg
      181 aagtgaacgg cttccaggga cccttcaggg cagcaagcac tggccatgtg taccagcgac
      241 gagcggtgac tcgtggctcc ccatccgggg agttaaagag cggtccgact atcccgcatc
      301 ttgggtgctc gtccatgaat cgagcgaggg tttcgacagc accgggggtg aatatcaagt
      361 ccgagttgca gagcatgatg tagggcgagc cggtttcgcg gatgccgcga ttcagattgg
      421 cgggaaggcc ccccggccga tcctgtgtga tgactcggac gttcggccac ttgcgcgaga
      481 tctgctctcc cgccccgtcg gtcgacgcgt tgtccaccac tacaacttcg agcggggtct
      541 cgccgacaga cgagaagagc gagtcgaggg cagggatcaa cacatgcccc tcgttggtcg
      601 ttacgaagac gagtgcgatg ggctcggtca tgccagcgcc ctttcgtagg tctcgatctg
      661 cagacgcgcc gatcgttccc agttaaaccc agaggccctc tccctgcctt tcgttgcgag
      721 attcgcggcg taaccatcgt cctcgagaac cctggagatg gtctcggtcc agccgtcgag
      781 gtcgtcgggg tcgaggagta ctccggcctc accgaccacc tccgggatgc atgtggcgtc
      841 ggacgcgatg accgggcatc cgaccgacat ggcctcgagt attggggccc cgaatccttc
      901 gaagcgcgat gggtagacga gtgcgcaagc actaaggtag agccccccga ggtcgcgtct
      961 gtcgatgagg ccaagctgtc taacgcgccc tgatagccca agtccccgtg ccgcatctgc
     1021 cagctcacga tctcgcggcc agggggcccc cgtaagcacg aggtcacacc tctccgagac
     1081 gggcttgtac gcctcgagca ggaagcgatg attcttgtga acgtagctcc tcgccgggta
     1141 gagaagaaat gggcgatcca acctgtatcg ggatctcgct tcgtcgggat cggccggggg
     1201 cccggcgaac ggatcctcgc tcccgaacgg aacgaccgtg actttctcgg cgtctgctcc
     1261 caactgctcg acgaggtcgc gcttcaccca ctcgctcggg acgctgacca tccgacagga
     1321 acgcagggtc atcggaacat tgaacgatag ccaccggagt ttcattgcgg cgaagttggc
     1381 ggggtagtgg cggtactgga tgtcgtggat ggtcgcaacc gatgggacgg tcttaagcag
     1441 tggcgtcgtt ccaacgccat ggtggatcac gtcgagcttc caccgtcgcg cctgccaccc
     1501 gagccatgtg ttctcggcgg cgatccgcag gccctgcctt ccgcgcgacc agggggcctc
     1561 caccagttga acctggtcga agagatccgg atacgtgcct cgcagtcccc taagaccgaa
     1621 gacgacgatc cgatgtcctg aagaactcat cgaccggacg aggttgacca acaaggcctc
     1681 agaggccccg acttcaccgg gaacgaggaa caggagattc acgcctatgc gcataacctg
     1741 aaggaggata tagacaaatg cgcttggcct tcgacgtatc tgcgatcctg gggaccagga
     1801 ccggtatcgg gcactactcg acgatcctgc ttgaacgact cgtctcgcac gatgcagata
     1861 tcgagatcca gcccttcgcc ctgacgacta agaaggatgt ctcgcggatc ccggagtcgc
     1921 tgcgtgagac ccttcgcagg atcagcgtgc cagcgaggct cggggttact gcttggacca
     1981 gggctgggcg tccctacgct gagacctatc tggacggggt agatgttgtc catgggccca
     2041 acttctgggt tccccctctc tcgagcgcat ccggggtcgt gacgatccac gacatgacct
     2101 tctcgcttta cccagagatg tgcacgcccc aggtgcgtcg ctacgcatgg atcatcccca
     2161 agatgttgaa ctggacgagg ctcgtgatca cgccgtcgga gtcggtccgt tcggaggttg
     2221 ccgaagtcat gggcttcccg cgtgaccgga tagtcgttac tcccgaaggc gtacgcggca
     2281 gtttcgtcgg cgtcgcaccc aaccaagatg ccgcgcgcga gtttggcgtc gatgggcctt
     2341 atgtgttatt cgccggcacg caggagcctc gcaagaatct cgaccgtttg atccaagcct
     2401 tcgcgatgct ctcagacgtt gaagttcaac tgttgatcgt aggtccgcca gggtggggga
     2461 gcgtcgatct tcaagctgta gccacgaaac tgcatgtttc atcgcgcgtt cacttcaccg
     2521 gttatgtgtc cgatgagcgg ttggcggttc tcatggcggg agcatcagcc ttcgcgttcc
     2581 cgagcatcta cgaagggttc ggactgccac cgctcgaggc gatggctgcc ggggtccccg
     2641 tcgtcgctgg tagagccggc tccctggaag agattctcgg tgaggccccc ttctggtgtg
     2701 acccgctcga cacgagcagc atcgccgacg cactgagccg ttgtcttcac gatgatgcgg
     2761 ctcggagcag agccaaagag gccggacgtt cgcgagcggg tctttatagc tgggatgaga
     2821 ctgcccgcct cactatcgaa gtctacgaac gcgctcaacg gatccgatga gatacttgct
     2881 caccccatgc cttctccgca gcgatctctc gccgacctga taaagcccgg gatggtccac
     2941 cctgggcgta ggaccagacg tcttggcgtc aggatcctca tcgggttgct gctcgttgcg
     3001 gtccttgggg ttctggctac cgccggtctc tacttcctcg gtgggccaaa ggcctctcat
     3061 gtgaaggggt tgggggagct gcccgcgggt gagccgatga atgtactcgt tctcggttct
     3121 gactctcgtg aggggctctc caaagaggaa ctgcgcaagt atgacccgca gggtaccgac
     3181 cgtcgttcgg ggcggcgctc cgacacgatc atgcttcttc acgttgaccc ggacaaggaa
     3241 aaggtcgtga tggtgagctt tccgcgagac ctgcgcatcc ggcacccgaa cggttccctt
     3301 ggccgcatca acgccatcta tcaacagggg ccgagtgcga tggtccaggc cgtgaagacc
     3361 tacacgggtc ttccgatcca tcactacgtc gaggtcaact tcgagggctt caagaacatc
     3421 gtcgatacgg tggggggcgt agatgtcttc ttcgaacggt ccatccggga gccggactca
     3481 ggactcaatg tacgcaaagg ctgtgtgcat ctcgttggcg accaggccct cgcgttcgtt
     3541 cgggtccgca agatcgactc ggatttcgga aggatcgcaa gacaacagct cttcctgcgc
     3601 ctcatgatgg acaaggttac tcacgccaag atcctcatca acccggtgaa gatcgtgaag
     3661 cttgtagaga tcctctccaa caacgtgact accgatacgg gcttgagtct cggggatctc
     3721 aagggcctag ccctgcaact tcggggattc gatcagtcac gcatagacat g
//
LOCUS       DATDOZ010000066         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1103832, whole genome shotgun sequence.
ACCESSION   DATDOZ010000066 DATDOZ010000000
VERSION     DATDOZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103832"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VI541_01245"
     CDS             complement(<1..793)
                     /locus_tag="VI541_01245"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HLF69561.1"
                     /translation="MNRGKTYFIRTFGCQMNEHDSERIEGLLQVQGFEKALAPDGVDL
                     VILNTCAIRENADKKLYGTLGQLKARKDEHPEMKIIVGGCLAEMDREGIVARAPWVDV
                     VFGTRNIEALPGLLRRVEEQGIPVVEFAETLSTFPSALPALRGSTTHAWIAIQFGCNN
                     SCTFCIVPSVRGVETSRRIGDILGEVSQVCADGVVEVSLLGQNVNSYGRDIYGTPRFA
                     DLLLALDDVDGLRRVRFTSPHPKDFREPVARAMAESSVVCEHLHLP"
     gene            complement(813..1841)
                     /gene="corA"
                     /locus_tag="VI541_01250"
     CDS             complement(813..1841)
                     /gene="corA"
                     /locus_tag="VI541_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HLF69562.1"
                     /translation="MATWRLHSPETQRTDPLPSTQRLRARAYLTDTGEVQDLEPERIS
                     DLLEKPGTFVWLDIENPVEDDFALITDEFGLHDLAVEDATKPYQRPKLEFYDDHLFMV
                     VHPMWAQDDQIARSEIDMFVGPRFLVTVRKDPVRSIEEARVRWESSPEMLKEGVGYII
                     YVLLDSIVDEYFVVADDLTERIEDLEDEVISPGKGSAVPELIFRLRKELVTVRRAVFP
                     MREVMNALSRRDSRLVSPDLEPYFQDIYDHVLRVSESIENQQELLSAALEIYLSAVSN
                     NLNAIMKRVTSWAAIIGVATAIAGIYGMNFELVPRSGGIFGFWFAIGLILLSTIGLYI
                     YFKARDWL"
     gene            complement(1882..2148)
                     /locus_tag="VI541_01255"
                     /pseudo
     CDS             complement(1882..2148)
                     /locus_tag="VI541_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
     gene            complement(2246..>2776)
                     /locus_tag="VI541_01260"
     CDS             complement(2246..>2776)
                     /locus_tag="VI541_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HLF69563.1"
                     /translation="NIAGVIAAELGTDIALAKRCTLLHDIGKAITHEVEGSHALIGAE
                     LARRLKESPAVVHAIEAHHAEVEQRTIEAVITQTADAVSSARPGARRESLETYVKRLE
                     RLEALATAFPGVEKCYAMQAGREVRVMVRSDQIDDLQSEVLARDIAKKIEEELQYPGQ
                     IKVMVIREHRVVEFAK"
BASE COUNT          541 a          831 c          836 g          568 t
ORIGIN      
        1 ccgggagatg aagatgctcg cagacaacag atgattcggc catcgctcgt gcgaccggct
       61 ctcggaaatc cttggggtgg ggcgatgtga atctcacacg acggagccca tcgacatcgt
      121 cgagagcaag gagcaggtct gcgaatcttg gtgtgccgta tatatcacgg ccgtatgagt
      181 tgacgttctg gccgagcaga ctcacctcga ccactccgtc cgcacagacc tggctcactt
      241 caccaaggat gtccccgatg cgtcgcgagg tctcaacgcc gcgcaccgat gggacgatgc
      301 agaaggtgca tgagttgttg cagccgaact ggatcgcgat ccacgcgtgg gtcgtggaac
      361 ctcgaagggc gggaagggca gaggggaacg tcgacagggt ctcggcgaat tcaacgacgg
      421 ggatcccctg ctcctcgacc ctgcgcagca gccccggcag ggcctcgatg ttgcgtgtgc
      481 cgaagaccac atcgacccat ggggcccgcg cgacgatgcc ctcgcgatcc atctcagcga
      541 ggcatccccc tacgatgatc ttcatctccg gatgctcatc cttgcgggcc ttgagttggc
      601 cgagggtccc gtagagcttc ttgtcggcgt tctcgcgtat ggcgcaggtg ttgaggatca
      661 cgaggtcgac gccgtcgggg gccagagcct tctcgaagcc ctgtacctga aggagccctt
      721 cgatccgctc ggagtcatgc tcgttcatct ggcacccgaa ggtcctgatg aagtaggtct
      781 taccgcggtt catcggggaa tcatcccaga cttcagagcc agtcgcgggc cttgaaatag
      841 atgtagagcc cgatggtgct gagcagtatc agcccgatgg cgaaccagaa gccaaagatg
      901 ccacctgatc gcggaacaag ttcgaagttc atgccgtaga tgccagcgat cgctgtcgct
      961 actccgatga tcgccgccca actcgtaact cgtttcatga tggcgttcag attgttcgaa
     1021 accgcagaga gatagatctc gagagcagca gagagtagct cctgctgatt ctcgatcgac
     1081 tcgctaactc gaagcacatg gtcgtagata tcctggaagt agggctcaag atccggtgac
     1141 accaggcggg agtcgcgacg cgacagggca ttcatgacct ctctcatggg gaagactgcg
     1201 cgcctgaccg tgaccaactc cttgcgtagc cggaagatca gttctggcac ggcgctaccc
     1261 ttcccaggag agatgacctc atcttcaagg tcttcgatcc tctcggtgag atcatcggca
     1321 acaacgaagt actcatcgac gatcgagtcg agaagcacgt agatgatgta gcccacgccc
     1381 tccttcagca tctcgggcga gctctcccac cgcactctgg cttcttcgat ggatcggacc
     1441 ggatccttgc gtacggtgac gaggaaacgg gggccgacga acatgtcgat ctcgctacgc
     1501 gctatctgat catcctgcgc ccacatcggg tggacgacca tgaagaggtg gtcgtcatag
     1561 aattcaagtt tcggccgctg gtagggcttc gtagcatcct caacggcgag gtcatggagg
     1621 ccgaactcat cagttatgag ggcgaagtca tcttcgacag ggttctcgat gtcgagccag
     1681 acgaaggtgc cgggtttctc gagtaggtca gagatccgtt ccggttcgag gtcctggacc
     1741 tcgccggtat ccgttagata agcgcgcgct cgaagccttt gggtgctggg cagtgggtca
     1801 gtcctctggg tctccggcga gtgtaaccgc caggtcgcca acctcttcca ctattagacg
     1861 gggaagccca ggacgaaggg tccgctcctc tatcttgagc cggatcgccg tgcggccctc
     1921 gccatcgatg aagatgtcct ggaaactggg gatgcagacc aggtcgatgc cgccaggcgc
     1981 caggaacccc ctggcgatcg cgatggcttt gacggcctgg ttgagcgagc cggcgccgat
     2041 aacctggatc tcgaccgacc ccacctgtcg aacgattccg gcgatagccc cagccacgga
     2101 gctggggttc gacttggatg agaccttgag gagaccgact gtttccactt ttccgaagcc
     2161 ctccgacgag tcctacggct catcatttcg agaaaagttc cggattccct gcctgaggat
     2221 tccttttttt ccgcttttac agaacctatt ttgcgaattc gaccacacga tgctcgcgga
     2281 tgaccatcac ctttatctga ccggggtatt gaagctcctc ctcgatcttc ttggcgatgt
     2341 cccgcgccag gacctcggac tgcagatcgt cgatctggtc tgagcgcacc atgaccctga
     2401 cttcccgccc ggcctgcatc gcatagcact tctcgacgcc tgggaaggca gtggccagag
     2461 cctcgagccg ctccagccgc ttgacatagg tttcgaggct ctcacggcgg gcccccgggc
     2521 gtgcactgga tacagcgtca gccgtctggg tgatgacagc ttcgatcgtg cgctgctcga
     2581 cctcggcgtg gtgggcctcg atcgcgtgga ccacggctgg agactccttg agcctacgag
     2641 cgagctcggc cccgatgagg gcgtgagacc cctcgacttc atgggtgatg gccttgccga
     2701 tgtcgtgaag cagcgtgcac cgcttcgcga gcgcgatgtc ggtgccgagt tcggccgcga
     2761 tcacgcccgc gatgtt
//
LOCUS       DATDOZ010000067         4093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_313442, whole genome shotgun sequence.
ACCESSION   DATDOZ010000067 DATDOZ010000000
VERSION     DATDOZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313442"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VI541_01265"
     CDS             <1..354
                     /locus_tag="VI541_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674998.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HLF69564.1"
                     /translation="IEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNWELRLDRRAS
                     EKRIFPAIDIEASGTRKEELLLTKEELSLVWKLRRVLHALEGVAGLELLIDKMRQTKG
                     NLQFLMEVSKQNTGD"
     gene            369..680
                     /locus_tag="VI541_01270"
     CDS             369..680
                     /locus_tag="VI541_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69565.1"
                     /translation="MGTRLADPFTIGLFAVLIVGAMLATFKIMGRASKGYEKARARRA
                     AGGPAIPCSTCGSSMEYAGVNEFKVAPGPLSADTDSLPLEVHRCPTCRKVEMFLPPAA
                     G"
     gene            739..960
                     /gene="rpmE"
                     /locus_tag="VI541_01275"
     CDS             739..960
                     /gene="rpmE"
                     /locus_tag="VI541_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009409245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HLF69566.1"
                     /translation="MKKGIHPEYMEATVHCSCGNTFKTRSTVSDIRSDLCSECHPFYT
                     GKQKLVDTGGRVERFQRKYGKRPMPNITP"
     gene            1000..1953
                     /locus_tag="VI541_01280"
     CDS             1000..1953
                     /locus_tag="VI541_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HLF69567.1"
                     /translation="MEGVMMRGPLTWAVACRKPDSQIAVESFDLPTTVKRHRWLAWPF
                     FRGVYVLVESLTIGMRALKISANYQLEEEQAPSAEAVPDADPASPPPKKGAEKAIGWS
                     LGIALVFFSAIFIALPALISKLGGRALGVHGTVSQNLLEGAIRIGFFLGYIMLISLVP
                     DIKRVFQYHGAEHKTIYAYENGDPLEPEVVDRYSTLHVRCGTNFLFIVMFLAIIGHFV
                     VDLALAGQPVWLKILSRVLMIPVIAGSSYEVIRAAGKNERSIVFRTASLPGLALQKIT
                     TRPPTRDQIEVAIKAMEAVVASSGAPVVPPGDAGGLAPGGL"
     gene            1976..3043
                     /gene="prfA"
                     /locus_tag="VI541_01285"
     CDS             1976..3043
                     /gene="prfA"
                     /locus_tag="VI541_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLF69568.1"
                     /translation="MFERLEELDARYEEVQRKLSDPALNTDPKQLRELGKQSAELGEI
                     VLPFRRLRAAEAELASAKELLNVEQEPAMRELIEQEIQAQRAIVEELEVSIKALLIPH
                     DPRDDKDVIVEIKAGEGGDESALFAGDLFRMYGRYAERKGWKLEVLSSDPSSIGGFKD
                     VTFAIKGKSAYSRMKHEAGVHRVQRVPETESQGRIHTSAAGVLVSPEAEEVEVEIDQK
                     EIQIDVYRSSGPGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLQNREKAMRILRSRL
                     LQAEIDRQNAEQAAERKSQVKTGDRSEKVRTYNYPQNRVTDHRVKVSVHNLPAVMDGA
                     LDEFIEVLIAQERAEILSEDQ"
     gene            3040..3885
                     /gene="prmC"
                     /locus_tag="VI541_01290"
     CDS             3040..3885
                     /gene="prmC"
                     /locus_tag="VI541_01290"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HLF69569.1"
                     /translation="MKLAQGSPVLDALNAARLALALVTESAQAEAETLLMVVLGCRRA
                     DLFILREPLSAQQAQLLEQMVQRRCGLEPLQYITGRAAFRGAEFEVGPGVLVPRPETE
                     LVVEHALALLPSTDGARVIDIGTGSGAIGASIAAERPRVSVWATEVSPQAAEWARRNF
                     ARLGVSVELFEGDLFCGLRGPFDLVVSNPPYLSSDEVDDLPFDVATHEPRIALTAGPT
                     GLEVPARIIEQSATRLAPRGALVMETSPTHASELASLFERVLREVAVHDDLGGNARIV
                     TGRGA"
     gene            3885..>4093
                     /locus_tag="VI541_01295"
     CDS             3885..>4093
                     /locus_tag="VI541_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HLF69570.1"
                     /translation="MRVVSMADADAVHTCVEVLDEGGIVVLPTDTVYGIAARIDRPSA
                     VKRIFELKSRPDALALAVLVADLEEA"
BASE COUNT          794 a         1218 c         1287 g          794 t
ORIGIN      
        1 attgaagaag gcggatcgct caccattatt gccaccgccc tggtcgacac cggttcccgc
       61 atggacgagg tcatcttcga agagttcaaa ggcaccggga actgggagct tcgtctcgac
      121 cgcagagcat cggagaagcg catcttcccc gccatcgaca tcgaggcctc tggcacccgt
      181 aaggaggagt tgctcctcac caaggaagag ctctcactgg tttggaaact gcggcgtgtc
      241 ctgcatgccc tcgaaggcgt tgcaggtctc gaactgctca tcgacaagat gcgacagacc
      301 aagggcaacc tgcagttcct catggaggtg agcaagcaga acaccggtga ctagccggtg
      361 ttgcatccat ggggacgcgt ctagccgatc cgttcacgat cggattattc gcggttctga
      421 tcgtcggcgc aatgcttgca acattcaaga tcatgggtag agcatcgaag ggctacgaaa
      481 aggcaagagc gaggcgagcg gcaggagggc cggccatccc gtgttcgacc tgcggctcgt
      541 cgatggaata cgcgggcgtg aacgagttca aggttgcgcc aggccccctt tcggcggaca
      601 cggactcctt gccgctcgaa gtccatcgat gccccacctg cagaaaggtg gagatgttcc
      661 tgcccccagc cgctgggtag ttcgcgtatc agaagtgctg tgctactctc gataggttca
      721 cgcttaggag taaatgtaat gaagaagggc atccaccccg aatacatgga agcgaccgtt
      781 cactgctctt gcgggaacac cttcaagacg cgttcgaccg tttctgatat ccgcagcgac
      841 ctctgctcag agtgtcaccc gttctatacg ggcaagcaga agttggtcga caccggaggc
      901 cgtgtcgaac gcttccagcg caagtacggg aagcgtccga tgcccaacat cactccctga
      961 tctgtggcat ccaaaccctc gataggaggg caagccgtca tggagggggt catgatgcgc
     1021 ggccccctga cctgggcggt ggcctgccga aagcccgact ctcagatagc agtggagagt
     1081 ttcgatcttc cgacaacggt gaagcgccat cgctggctcg cttggccgtt cttccgcggg
     1141 gtgtatgtgc tggtggagtc tcttaccatc gggatgcggg cgctcaagat atcggccaac
     1201 tatcagctcg aagaagagca ggcaccgtcc gctgaggccg tgcctgatgc cgaccccgcg
     1261 tcgccgccac cgaagaaggg tgccgagaaa gcgatcgggt ggagtctcgg tatcgccctt
     1321 gtcttcttca gtgccatctt catcgctctg ccggcgttga tatcgaaact tgggggccgg
     1381 gcactcgggg ttcacggaac cgtctcgcag aatctgctcg agggggccat ccgcatcggc
     1441 ttcttcctcg gttacatcat gctcatctca ctcgtgcccg acatcaaacg cgtcttccag
     1501 taccacggtg cggaacacaa gacgatctat gcatacgaga atggcgaccc gctcgagccc
     1561 gaggtggtcg atcgctactc gactcttcac gttcgctgtg ggaccaactt cctgttcatc
     1621 gtgatgttcc ttgcgatcat cggccacttc gtggtcgatc tggctctcgc aggacaaccc
     1681 gtctggctca agatcctctc ccgcgttctg atgatccctg tgatcgccgg ttcgtcatac
     1741 gaggtcatcc gggctgctgg caagaatgaa cgctcgatcg tctttcgtac cgcaagcctt
     1801 ccggggctcg ctcttcagaa gatcaccacc aggcccccga cgcgcgatca gatcgaagtg
     1861 gcgatcaagg cgatggaggc tgtcgtggca tcgagtggtg cgccggttgt gccgcccggt
     1921 gatgctgggg gcctggctcc cggcggcctc taacttcacc taacctcgga acccgatgtt
     1981 cgagagactc gaagagcttg atgctcgcta tgaggaggtt cagcgaaagc tgagcgaccc
     2041 tgcgctcaac accgacccca agcagctgcg tgaactcggc aagcagagcg ccgagttggg
     2101 cgagatcgtc ctgccgttcc ggcgtcttcg cgcggctgag gctgagctcg catcagcgaa
     2161 ggaacttcta aatgtcgaac aggaacctgc gatgcgcgag ttgatcgaac aggagatcca
     2221 agcccagcgc gcgatcgtcg aagagctcga ggtgtcgatc aaggcccttc tcatcccgca
     2281 cgacccgcgc gatgacaaag atgtcatcgt cgagatcaag gctggcgaag gcggggacga
     2341 atctgcacta ttcgccgggg acctgttccg catgtatgga aggtatgcgg aacgcaaagg
     2401 ctggaagctc gaagtgttgt ccagcgaccc ttcgtcgatc gggggattca aggacgtcac
     2461 atttgcgatc aagggtaaga gcgcctactc gcgcatgaaa cacgaggccg gagtccatcg
     2521 cgttcagcgc gttcccgaga ccgagtcgca gggccgcatc cacacatctg ccgctggtgt
     2581 gctggtatct cccgaggctg aggaggtcga ggtcgagatc gatcagaagg agatccagat
     2641 cgatgtctat cggtcgagtg gccctggggg ccagtcggtt aacaccaccg actcggccgt
     2701 tcggataacc catctcccga ccggcctcgt ggtcacgtgc caggacgaga agtcccagtt
     2761 gcagaaccgc gagaaggcca tgcggatact gaggtcgcgg ctcttgcagg cggagataga
     2821 tcgtcagaac gccgagcagg ccgccgagcg caagagccag gtcaagacag gcgatcgatc
     2881 ggagaaggtc cggacctaca actatccgca gaaccgagtg accgatcatc gggtcaaggt
     2941 gtcggtccac aatctccccg cggtcatgga tggcgcactc gacgagttca tcgaggtcct
     3001 catcgctcag gagcgggcgg agatactttc ggaggatcag tgaagctcgc gcagggttcg
     3061 ccggtcctcg atgccctgaa cgcggctcgc ctcgctctgg ctttggtgac ggagtcggct
     3121 caggccgagg cggagaccct tctgatggtg gtgcttggat gtcggcgcgc cgatctgttc
     3181 atcctccgag aaccattgag cgcgcagcag gctcaacttc ttgagcagat ggtccagcgt
     3241 cgatgcggcc tcgaaccgct gcagtacatc accggaaggg ccgcgttccg gggggccgag
     3301 ttcgaggtag gtccgggggt cctggtgcct cgaccggaga ccgagctggt cgtcgaacat
     3361 gcccttgcgc tgctcccgtc tactgacggt gcgcgagtca tcgacatcgg tacgggatcg
     3421 ggggccatag gggcctccat cgctgcggag cgccctcgcg tatcggtctg ggcgacggag
     3481 gtcagtccgc aggcggccga atgggcgcgg cgaaacttcg ccaggctggg cgtttcggtg
     3541 gaactcttcg agggagatct cttctgcggt ctgcgggggc cgttcgatct cgtcgtgagc
     3601 aaccctccct acctctcgtc cgatgaggtc gatgacctgc cgttcgatgt ggccacacat
     3661 gaaccgagga tcgccctcac cgccgggccc acggggctcg aggtccccgc aaggatcatc
     3721 gagcagtcgg cgacccgcct ggctcctcgg ggggccctcg tgatggagac ctctccgacg
     3781 catgcgtccg aacttgcatc gttgttcgag agagtgttgc gtgaagttgc agtccacgat
     3841 gacctcggag gaaacgctcg gatcgtgacc gggcgcggag cttgatgcgc gttgtctcca
     3901 tggcggacgc cgatgcagtc cacacctgcg tggaggttct cgacgagggc ggcatcgttg
     3961 tcctgccgac ggataccgtc tacggcatcg cggcacgcat cgaccgcccc tcggctgtga
     4021 agaggatctt cgagttgaaa tccagaccag acgctcttgc gttggcggtg ctggtcgctg
     4081 atctggaaga ggc
//
LOCUS       DATDOZ010000068         2858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_313863, whole genome shotgun sequence.
ACCESSION   DATDOZ010000068 DATDOZ010000000
VERSION     DATDOZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313863"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VI541_01300"
     CDS             <1..1204
                     /locus_tag="VI541_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HLF69571.1"
                     /translation="PGMRVTIAFDDPTVPCYAPLWAIALPEIISTLYAAGVEKKDLTL
                     VCANALHRKFTHDELARLIGLELVAEFGPQLLCHDAEDATNMVALGRTPSAYHVDLNR
                     LAVESDLVIYLNCSTTRGFSGGWKSICVGLASYRSIAHHHTPEIMSMSLDRNEMHEML
                     DEMGAFTEATLGSERFFKLETVLANPLQVAAIFGGSVGATRRAALDLNRANLTNRRDL
                     LAQKADIVLYGVPDWSPYAAFSFNNPILTLISTGLGYLGGMIEALGKPGCTVILATPC
                     EDRWDHQHHPSYKEVWDRVLPVTTEPGEARRMFEAEFATRSDYIDRYRTEYGFHGVHG
                     IMALYPLKRLRHASRVIAAGADPALARHVGFTPSATIEDAISEARLEHGSDAVIACVK
                     YPPGVSRQ"
     gene            1201..2502
                     /locus_tag="VI541_01305"
     CDS             1201..2502
                     /locus_tag="VI541_01305"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLF69572.1"
                     /translation="MTAARLFPRAAVASPHYLATTAGVHVLMQGGNAVDAAIATNLVL
                     AVVCPHLCGVGGDLFALIFAEGRIHGLNSSGCLPKAATLPPDGQVPVRGVGAATVPGA
                     VAGWLAMHDRFGSMPIGNLAAPAIRYAQQGFPRTSSLRRSITRSLDVFDDGTDFARLF
                     LRDEPTGRNVDLAATLGDLSNFYSGPVAQNAPAPFTPDDFSSHQAQWVDPVTTPFRGH
                     DIYEMPPNSRGHLVLRALDRLEPLEGSGPDDAQFHERLFRAIASVSLTGDTVYLCVHD
                     EKGMAVSLNESNFMGFGSGVLVPGTGVHLHNRGAYLTPATYKGGVRPIHTLSPGMSLL
                     DGQPSLIFGTMGGEAQIQIHMQLLARVLVAGQELGAAVAAPRWVKHGEGQLLAEEGLP
                     EMGATVIPRSDIAGHAHMIRRTDEGIEAVSDPRSDGLALGY"
     gene            2612..>2858
                     /locus_tag="VI541_01310"
     CDS             2612..>2858
                     /locus_tag="VI541_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69573.1"
                     /translation="MVSLKKNKDASSSAPTQATAIKKPRKEKRWDELVRKYPWLKKLS
                     KLKPRWPGFKKLFGPLLLFLIASLFISYGFILRAIQPV"
BASE COUNT          554 a          933 c          831 g          540 t
ORIGIN      
        1 acccgggatg cgtgtgacga tcgcattcga cgatccaacc gtcccctgtt atgcaccgct
       61 ttgggcgatc gcactgccgg agatcatctc caccctctac gccgcgggcg tcgagaagaa
      121 ggacctcacc ttggtatgcg ccaatgccct gcaccgcaag ttcacgcacg atgaactcgc
      181 tcgcctcatc ggcctcgaac tggtcgccga gttcggcccc caactgctct gccacgacgc
      241 ggaggatgcc accaacatgg tcgcacttgg tcgaaccccg agcgcatacc acgtcgacct
      301 caaccgtctt gccgtcgaga gcgaccttgt tatctacctg aactgctcaa cgacccgcgg
      361 gttcagtggg ggctggaagt cgatctgcgt cgggctggcc tcttatcgat ccatcgccca
      421 tcaccacacc cccgagatca tgagcatgtc gctcgaccgc aacgagatgc acgagatgct
      481 cgatgagatg ggggccttca ccgaggcaac gctcggaagc gagaggttct tcaagctgga
      541 gactgtgctc gcaaaccccc ttcaagtagc agccatcttc ggagggtcgg tcggagcgac
      601 gcgacgcgcg gccctcgatc tgaaccgagc caatctgacc aaccgtcgcg acctgctcgc
      661 gcagaaggcc gacatcgttc tctatggagt gcccgattgg agtccttacg cagcattctc
      721 attcaacaac ccgatactca cactcatctc gaccgggctt ggctacctcg gtgggatgat
      781 cgaagcactt ggcaagccgg ggtgcacggt gatcctagcg actccctgcg aggaccgttg
      841 ggatcatcag caccacccct cgtacaagga ggtttgggat cgcgttctac ctgtcactac
      901 cgaacccgga gaggcacgga ggatgttcga ggcagagttc gccacgagga gcgactacat
      961 agatcgctat cgcaccgagt acggcttcca cggggtccac ggcatcatgg ccctgtatcc
     1021 attgaagagg ttgcgtcatg caagcagagt gatcgcggct ggcgcggacc cggctctcgc
     1081 gagacatgtc ggcttcacac cgtcagcgac gatcgaagat gccataagtg aagctcgact
     1141 cgagcacgga agcgatgcag tgatcgcgtg cgtaaagtac ccaccaggag tgagtaggca
     1201 gtgaccgccg ctaggctctt cccgcgagcc gcggtggctt ccccccacta cctcgcaaca
     1261 accgctgggg ttcatgttct gatgcagggt ggcaacgcgg tcgacgcggc gatcgcgacc
     1321 aacctcgtgc tggcggtcgt ctgtcctcat ctctgcggcg tcggtggaga cctcttcgca
     1381 ctcatcttcg ccgaggggcg gatccatggg ctcaactcgt ctgggtgtct accgaaagca
     1441 gccaccctcc cgccggatgg gcaggttccc gtaaggggcg tcggcgctgc caccgtcccg
     1501 ggcgccgtgg ccgggtggct cgcgatgcac gaccgattcg ggtcgatgcc tatcggtaac
     1561 ctcgcggccc ccgccatccg ctatgcccaa cagggcttcc cccgcacctc gagtcttcga
     1621 aggtccatca cgaggtcgct cgacgtcttc gacgatggca ccgacttcgc gcgactcttc
     1681 cttcgcgacg agccgaccgg ccggaacgtt gatctcgcgg caacccttgg cgacctttcg
     1741 aacttctact ccgggccggt cgcgcagaat gctccggccc ctttcacgcc cgacgacttc
     1801 tcgagccacc aagcccagtg ggttgacccc gtgaccacac catttcgggg ccatgacatc
     1861 tacgagatgc ccccgaactc gcgcggtcat ctcgttcttc gggccctcga ccgcctcgag
     1921 ccactagagg gatcggggcc ggacgatgcc cagttccatg aacggctctt tcgcgcgatc
     1981 gcatccgtct cgctcaccgg cgacacggtc tatctctgcg tgcacgatga gaagggcatg
     2041 gctgtgagcc tcaacgagag caacttcatg gggttcggat ctggggtcct ggtgcctggc
     2101 acgggcgtgc acctgcacaa ccgcggggcg tacctcacgc cggccacata taaaggaggc
     2161 gtgcgcccaa tccataccct ctcgccgggc atgtcgctgt tagatgggca gccatcgctc
     2221 atcttcggga cgatgggagg cgaggcacag atacagatcc acatgcagct tctggctagg
     2281 gtgctcgtgg ctgggcagga gttgggggca gccgtggccg ctcctcgctg ggtgaaacat
     2341 ggtgagggtc aactcctggc cgaggagggt ctccccgaga tgggagccac ggtcataccg
     2401 cggtcggaca tcgcaggcca cgcacacatg atccgcagaa cagatgaggg catcgaagca
     2461 gtctcggacc cacgatccga cgggcttgcg ttaggatatt agggttcggc gactattcga
     2521 cagagtacgc tcgaaccatc atcatggttc ggttgtctca acaactccaa tcgccaccca
     2581 cactgtccat cttgaacaag gagcctgtca gttggtttcc ctaaagaaga acaaagatgc
     2641 ctcttcgtcc gcgccgaccc aagcaaccgc gataaagaag cccaggaagg agaagcgctg
     2701 ggacgagctt gttcgcaagt acccttggct caagaagctc tcgaaactga agccgcgttg
     2761 gcctggcttc aagaaactgt tcggccccct gctcctcttc ctgatcgcgt cgctattcat
     2821 ctcgtacgga ttcatcctgc gggccataca gcccgtcc
//
LOCUS       DATDOZ010000069         4233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3449237, whole genome shotgun sequence.
ACCESSION   DATDOZ010000069 DATDOZ010000000
VERSION     DATDOZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449237"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VI541_01315"
     CDS             complement(<1..331)
                     /locus_tag="VI541_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69574.1"
                     /translation="MKKTKLLVSLISVMVLMLAGIGSPAQADMPTRPGLYAATSVPQV
                     LPAPDITGGDPSAVGLWSSPFAENPLFNTTPPATNAESKQLPTAVSSVVLKDGRILYW
                     NGLEGTED"
     gene            447..596
                     /locus_tag="VI541_01320"
     CDS             447..596
                     /locus_tag="VI541_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69575.1"
                     /translation="MSDEKVPRGPDLGDEAPDAEVFRADGSSVLLASLFSRGPVALIF
                     LRHYG"
     gene            612..1016
                     /locus_tag="VI541_01325"
                     /pseudo
     CDS             612..1016
                     /locus_tag="VI541_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
     gene            complement(1006..1737)
                     /locus_tag="VI541_01330"
     CDS             complement(1006..1737)
                     /locus_tag="VI541_01330"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HLF69576.1"
                     /translation="MRTGRTWAIARADGSVQTGTFGTSRFQRIPLLRVIFGIFGALAI
                     TIRRVGRGKQNRKFFFALLALMVAMWPLDSWVHHADLPGWAQAIYGPLSLLLVIGLLK
                     VAAPASLWRYHGAEHKAVAAYERDISLDDPDAVMLCSRIHDRCGTNLVFLIGVISLLP
                     LPSGGMGALTMLALIGLVAEVLGFAVRQHPRSILTRILLGGGRFLQRALTTREPQRDE
                     VTVACTALKAALAEHANLALRAATG"
     gene            1814..2389
                     /locus_tag="VI541_01335"
     CDS             1814..2389
                     /locus_tag="VI541_01335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF69577.1"
                     /translation="MTDSRRDYEQVYSELTWAYGDKPDLELIHALEGRPRGRAVDVGG
                     GQGRHALPLAALGFDVVVVDTAAAGLHQGAESAAEKDLNIHFVQQDAAQFEYGPGLKA
                     AVAALFFHIPAHKTSISIAQRIGEALEPGSPLYLSVPGFTSETVELVEAVIEAAGCKK
                     DFIVKHLVTKKERPRLSVPRRNETRALGIKP"
     gene            complement(2390..>4233)
                     /locus_tag="VI541_01340"
     CDS             complement(2390..>4233)
                     /locus_tag="VI541_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69578.1"
                     /translation="TVEPPLIKESRRARAKRLRRNITRGLHLAWMSSPRSVVIILAMN
                     AVAALMPPAAVWLGKELARVIRVGFATQQPFAFALPVVAALGAVAAAQRVIGILQATR
                     QELFQRRVVLEVEYRFLQQAASVDLGHFDNSDWHDRVARAKRDMNWKPAQLTQSIIGL
                     LGAVITTAGMLGLLLTLHPLLVVMSLLSVIPSLTIQRRVTRKIYKFFFTNTPEERERD
                     YIADLVSQPKTSKEIRAFNLSDYLLKRRKDLAEDLFAEHSRLYRSANRAYGWSGILSG
                     IALGVAYAFVASRGLAGQLRPEDLIVVIGAFASVTGQIGLISGSLIAIDQHATFLDDY
                     FSFMEIETLVPSPAPSPSPITLARSQIELHGIEFSGVQFTYPGGTQPALDGLDLHIHC
                     GEMLALVGENGAGKTSLVKLLLRFYDPQQGSIKIGGVDLRDLRPDVLRDRIGILFQDY
                     AMYELSIRENVILGRADTQDHDDVLVMEALRRARSEWLVDQMPKGLDSKVGRLFEGGH
                     DLSGGEWQRLALGRLMYRDADIWILDEPTASLDPEAEAGIFAELRENLRGRMGIVISH
                     RFSTVRIADRIAVIADGGVTELGTHEELMAAGGRYSELFELQASGYR"
BASE COUNT          817 a         1280 c         1359 g          777 t
ORIGIN      
        1 ggtcctcggt cccttcgagc ccgttccagt agaggatccg tccatcctta agtacaacag
       61 atgagacagc ggttggcagc tgtttggact ccgcgtttgt ggccggggga gtggtgttga
      121 acagcgggtt ctcagcgaac ggcgatgacc acagaccgac agcgctcgga tcgccgcccg
      181 tgatgtcagg tgcgggcaaa acctgaggta ccgatgtcgc ggcgtacaga cctggtctcg
      241 tcggcatgtc agcttgagcg ggcgagccga tgccagccaa catcaacacc atgaccgaga
      301 tgagcgatac gagcagcttg gtcttcttca tgcctaagaa cctcccccgg ggtatcgaga
      361 ctctttctac atcaggcccc ccttctcctg cctggtcggt gaagttccct gaggacgaag
      421 tgggccaaac ctgtagcctt ggtcccatga gtgacgaaaa ggtcccgcgg gggcccgact
      481 taggcgacga ggcccccgat gccgaggtgt tccgtgccga tggctcctcg gtcctgctcg
      541 ccagcctgtt ctcgcgggga cccgtcgcgt tgatattcct gcggcactac ggctgaacgt
      601 tctgcaagca acacatcgcg cagttgcgcg atgccaaaga tgagttcgat gctgctgggg
      661 tcggaatagt gatggtcggt caatcaacac ccgaggacct gtcgaagttc ctggccagcc
      721 gaaagctgcc cttcgagatc tactgcgacc caggcaacaa gacctatcgt gcctacggcc
      781 ttgagcgcgg ctcactctgg caggtcacta cgtcgcctaa ggtactgctg aagggggcca
      841 tcgcccacgc gcagggtcac atgcagaccg gcatcgtcgg cgatgcgatg cagatgccag
      901 gttcgttcgt gatcgtcgag ggcaagatcg tctacgcgca tagagggcag atgtcctcgg
      961 accttgcacg ccccgccgaa ctgctcacgg ctgcgactcg aagccctagc ccgtagctgc
     1021 tcgcaacgcg aggttggcgt gctcggcgag agctgccttc agcgcggtgc aggcaacggt
     1081 cacctcgtcg cgctgcggct ctcgtgtcgt tagcgcgcgc tgaaggaatc gtccgccgcc
     1141 gagcaggatg cgtgtgagta tcgagcgagg gtgttgacgc accgcgaagc cgaggacctc
     1201 tgcaaccaga ccgatgagtg ccagcatggt cagggccccc atgccccccg atggcagcgg
     1261 cagcagtgag atgaccccga tcaagaagac gaggttggtc ccgcagcggt catggatccg
     1321 agagcagagc atcaccgcat cggggtcgtc gaggcttatg tcgcgttcgt atgcggcaac
     1381 ggctttgtgc tctgcgccgt gatacctcca gagcgacgct ggtgccgcga ccttgagaag
     1441 cccgatgacc agcagcagcg agagcgggcc atagatggcc tgcgcccacc cgggcagatc
     1501 cgcatgatgt acccaactgt ccagtggcca catggcgacc atcagagcga gcaaggcaaa
     1561 gaagaacttt cgattctgtt tcccccgtcc aactcgacgg atcgtgatgg ccagggcccc
     1621 gaagatcccg aagatgaccc gcagcagggg gatcctttgg aatcgtgagg tcccaaaggt
     1681 tccggtctga acgctcccat ccgcgcgggc gatggcccag gtccggccgg tacgcatcaa
     1741 gactccgtca gcgagggcct ggcccccaac ccgcaatcgc atcccccaag gataagggat
     1801 agccttgcaa gtgatgactg actcgcgccg cgactatgag caggtctaca gcgaactcac
     1861 ctgggcgtat ggcgacaagc ccgacctcga actcatccat gcactcgaag gcagaccgcg
     1921 cggacgagca gtcgatgttg gcggcggtca gggaaggcac gcgttgcccc ttgcggcact
     1981 cggcttcgac gtcgtcgtcg tcgacactgc agcggccggt ctgcaccagg gggccgagtc
     2041 ggcggctgag aaggatctga acatccactt cgttcagcag gacgcagcgc agttcgaata
     2101 cggtccgggc ctcaaggctg cggtggccgc gctcttcttc cacatccctg cccataagac
     2161 ctctatcagc atcgcgcagc ggatcggcga ggccctcgaa ccggggtcgc cgttatacct
     2221 atcggtcccc ggcttcacat ccgagacggt cgagttggta gaggcggtca tcgaggcagc
     2281 gggatgcaag aaggacttca tcgtcaagca tcttgttacg aagaaggaac gtccccggct
     2341 gtcggtcccg aggcgcaacg agacgagggc cctcggcatc aagccctgac tagcggtacc
     2401 ccgaggcctg taactcgaag agttcgctgt acctgccccc cgccgccatg agttcctcat
     2461 gagtgccaag ttccgtcacc ccgccatccg cgatcaccgc gatgcggtcg gcgatgcgca
     2521 ccgtcgagaa gcggtgtgag atgacgatcc ccatgcggcc cctgaggttc tctcgaagct
     2581 ccgcgaagat cccagcctcg gcctcggggt cgagtgatgc ggtgggctcg tcgaggatcc
     2641 agatgtcggc atcgcgatac atcagtcggc ccagcgcgag gcgctgccac tcgccgcccg
     2701 aaagatcgtg gccgccctcg aacagacggc ctaccttcga gtcgagaccc ttgggcatct
     2761 gatcgacgag ccactcggag cgcgctcgac gcagcgcttc catcacaagg acatcgtcat
     2821 gatcctgcgt gtcagcgcga ccgaggataa cgttctcgcg aatgctgagt tcgtacatgg
     2881 cgtagtcctg gaagagtatg ccgatgcgat cgcgcagaac atcgggcctt aggtcacgaa
     2941 ggtccacgcc tccgatcttg atggaccctt gctgtgggtc gtagaagcga aggagcagtt
     3001 tcacgaggct ggtcttgccg gcaccgttct cgccgacgag ggcgagcatc tcgccgcagt
     3061 ggatgtgaag gtcgagcccg tctagcgcag gttgggtccc ccctgggtag gtgaactgga
     3121 ccccggagaa ctcgatgcca tggagctcga tctgagatct cgccaatgtg atggggcttg
     3181 ggctgggggc aggtgaggga accagtgttt cgatctccat gaacgagaag tagtcgtcga
     3241 ggaatgtagc gtgctggtcg attgctatca gcgacccaga gatgagcccg atctggccgg
     3301 tgaccgaggc gaatgccccg atgaccacga tgaggtcctc gggtcgcagc tggcctgcca
     3361 agcctcgcga ggcgacgaac gcgtatgcga cgccgagggc gatgccgctg agtatccccg
     3421 accatccgta ggctcggttt gcagacctgt acagccgcga gtgctcggcg aagagatcct
     3481 ccgcgaggtc cttgcgtcgt ttcaggaggt aatcggagag gttgaaggcc ctgatctctt
     3541 tggatgtctt gggttgactg acgaggtcgg cgatgtagtc gcgctcgcgc tcttcaggtg
     3601 tattggtgaa gaagaacttg tatatcttgc gcgtgaccct gcgttggatg gttaaggatg
     3661 ggatcactga aagaagtgac atcacgacca gcagaggatg gagagtgagc agaagtccga
     3721 gcatcccggc tgtggtgatg actgccccca gtagcccgat gatcgactgg gtcaactgag
     3781 caggcttcca attcatatcg cgcttggccc tcgccacgcg gtcgtgccag tcggagttgt
     3841 cgaagtgtcc gaggtcgacc gacgccgcct gctggaggaa tcggtactcg acttcgagga
     3901 caactcgccg ctggaagagt tcctgacggg tggcctgcag tatcccgatg acgcgctgtg
     3961 ccgcggctac tgcgcccaga gccgcgacga cgggcagtgc gaatgcgaat ggctgctgcg
     4021 tagcgaaccc aacgcggatg actcttgcca gttccttgcc caaccagacc gctgcggggg
     4081 gcatcagcgc ggcgaccgcg ttcatcgcaa ggatgatcac gaccgagcgg ggcgaagaca
     4141 tccacgcgag gtgaagcccg cgggtgatgt tgcgccgcag tcgcttcgcg cgtgcgcgtc
     4201 tcgactcttt gatgaggggc ggctcgacgg tgt
//
LOCUS       DATDOZ010000070         4562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3449817, whole genome shotgun sequence.
ACCESSION   DATDOZ010000070 DATDOZ010000000
VERSION     DATDOZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449817"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..872
                     /locus_tag="VI541_01345"
     CDS             156..872
                     /locus_tag="VI541_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HLF69579.1"
                     /translation="MREMQLWKSIFRPGSIFQKGHQDSPRNRAYVVMNSVLYHLHPVK
                     VKRHGIKLSYTLCLGGLSFFLFIFLTVTGIFLMFFYRPSTEVVYSDMFALHTSVAFGL
                     LVRNLHRWGAHLMTISVFLHMARVFYTGSYKPPREFNWILGVTLLLVTLLLAFTGYLL
                     PWDQLAIWAVTVGTQMAYESPLIGKEVSFVLLGGVQIGASTLIRWYTLHVLALPFVIV
                     IQMAVHFWRVRKDGGISGPL"
     gene            891..1697
                     /locus_tag="VI541_01350"
     CDS             891..1697
                     /locus_tag="VI541_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HLF69580.1"
                     /translation="MPETPDEIYDKVLAEEQGKGSSPAVAEARAKAARHRARKGLPTD
                     PKAAAAASGGTAPVSEPEPTQADASQETPVAAAVGAAPAGRAGRAPSAGPDVAPERIQ
                     RLLAVVRPEAIQRVEREPIDRVNVWPHLLAIEMVALLAMTAFLVIYSTFINAPFRELA
                     NINVTPNPSKAPWYFLGLQELLRYFHPQIAGVTIPQWIILGLMAAPYIDRNPSVKPKD
                     RKLAIAIFTIFLLMNAVLVILGSIFRGPGFNFVYPWSCGEGAKNCIFFEL"
     gene            1694..2482
                     /locus_tag="VI541_01355"
     CDS             1694..2482
                     /locus_tag="VI541_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HLF69581.1"
                     /translation="MSPNVITALVVIVSLGAWALFMAYTALGAKKGSHGIGIPLPEPV
                     EGTVPIEGAPGFMRETTVSANKKARRTDAELQGVSRRQFLSKAWVGGIIVAFAQFGLA
                     SLDFLYPRLRGGFGAKITVGDVEEYRQLISAARQPVFIPDGRFWLTIFEGDAKTAEKV
                     PTYKLANVHETGFMALYRKCVHLGCSVPWCDSAKFFECPCHGSKYSVNGEWRDGPAPR
                     SLDQFRVEIVGGKLVVDTSSVIEGAPRGTITSQPQPEGVHCVNV"
     gene            2512..3426
                     /locus_tag="VI541_01360"
     CDS             2512..3426
                     /locus_tag="VI541_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF69582.1"
                     /translation="MRQVAIAVVILGFLAIAIAVLTRRGPRGAERVAPALRPGPSDDV
                     LEDERLTKTMRWGVVIVLFFAIFLPIYWFAEPGRMDAKTKAFEEESISRGKAYYALRV
                     DQETGEENHEGVECARCHGSEAQGGETQFLDPNSGQVRNVKVPSLFDVFARYETAPPG
                     FKDARAFILETIERGRPGTAMPTWGNENGGPLTEQQIDDIINYLYAIQHAPEVKAQAT
                     GADIFSQNCSPCHGIGGTGGAGPAMVNGAVAAQFPTIEDHIAFVKAGSKQGVKYGTNG
                     KGTGGMPAWGKLLTDAQIQLVVEYERSL"
     gene            3427..4386
                     /locus_tag="VI541_01365"
     CDS             3427..4386
                     /locus_tag="VI541_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HLF69583.1"
                     /translation="MDPVFVKGIVAFALMILVFIGSVYLLLSMILGARLGYLVLGSCF
                     FGVMILIALIWMVTALGPKGPETTWVTVRTGPSLSTIESEFGNFDVSSYPNGWVDPSP
                     DGHLADLSGEDNTQTELENVKPVLSLLVDEAISPIPGIRKRVADQVDGSISLETGHFE
                     VIDVRMKEAKVNGKDSIIAVGRAVSSATLSAVEFSNGAKEGTVARFLVSNGDAVGQGT
                     PLVEVTTDTGTIALVSPKDGKLIAFGLRKGDKIKPGVPFAKLDVTGQPGAAEPALVAG
                     VRVRGAVRTTALYYLAACTLLFALHMAALSRSEKARKTVPQPA"
BASE COUNT          912 a         1393 c         1355 g          902 t
ORIGIN      
        1 cgatcgggaa actcggagca accaagtcgg gagctcgcgc tttgggtatc gttgccggcg
       61 agcagggcga gtgaggggaa tctgtgtcta gagatctcaa agagaagaag cgctttccga
      121 atccagcgga agagatgaag aagcgcttcg agcagatgcg cgagatgcag ctatggaagt
      181 ccatcttccg tcccggctcg atcttccaga agggacacca ggactctccg cgcaaccggg
      241 catacgtcgt gatgaactcg gtcctatacc acctgcaccc ggtgaaggtt aagcgccacg
      301 ggatcaagct ctcctacacc ctctgtctcg ggggcctctc gttcttccta ttcatcttcc
      361 tgaccgtgac aggcatcttc ctgatgttct tctacaggcc gtcgaccgag gtcgtctact
      421 cggacatgtt cgccctccac acatcggtgg cgttcggcct tttggtacgt aaccttcaca
      481 ggtggggcgc gcacctcatg accatctcgg tcttcctgca tatggccagg gtcttctaca
      541 ccggctccta caagcccccg cgggagttca actggatcct cggtgtaacc ctcctgctcg
      601 tcacgctgct acttgcattc accggatatc tgttgccttg ggaccaactc gcgatctggg
      661 ctgtgacggt aggaactcag atggcttatg aatcgccgct cataggcaaa gaggtctcat
      721 tcgtcttgct gggcggggtt cagatcggag catcgacgct catcaggtgg tacacgctgc
      781 atgtcttggc actgcccttc gtcatcgtca tccagatggc cgtccacttt tggagggtcc
      841 gtaaggatgg cggcatctca ggcccccttt aggccgatag gatcgagaac atgcccgaaa
      901 ctcccgacga gatatacgac aaggtcctag ctgaagagca ggggaagggt tcttcgccgg
      961 cagtggccga ggcgagggcc aaagccgctc ggcatcgggc taggaagggt cttccgactg
     1021 acccgaaggc cgcagccgct gcaagcggcg gtacggcccc cgtcagcgaa cccgagccca
     1081 ctcaggcaga tgcctcgcag gagactcccg tggccgctgc cgtcggtgcg gcccccgcag
     1141 gacgggccgg gcgggcgcca tccgcaggac cggacgtcgc tcctgagcgc atccagcgac
     1201 ttctagcagt ggtgcggccc gaggcgatcc agagggttga gcgcgaaccc atcgataggg
     1261 tcaacgtgtg gccccatcta cttgctatag agatggtggc gctcctggcg atgactgcat
     1321 tcctggtgat ctattcaacc ttcatcaacg ccccattccg agagctcgcc aacatcaacg
     1381 tcaccccgaa cccctcgaag gctccctggt acttccttgg tctgcaggag ctcctgcgct
     1441 acttccaccc acagatcgcg ggtgtgacga ttccgcagtg gatcatcctg gggctcatgg
     1501 ccgcgccata catcgatagg aacccttcgg tgaagccaaa ggaccgcaaa ctcgccatcg
     1561 cgatcttcac gatcttcctg ctgatgaacg ccgtgttggt catcctcggt tccatattcc
     1621 gcgggcccgg tttcaacttc gtctacccct ggagctgcgg cgaaggggcc aaaaactgca
     1681 tattcttcga gctatgagtc caaacgtaat cacggctctc gtcgtcatcg tttccctggg
     1741 tgcctgggca ctcttcatgg cctacaccgc gctcggtgct aagaagggtt cgcatgggat
     1801 agggatacca ctaccggaac cggtcgaagg aaccgtcccg atcgaggggg cccctggctt
     1861 catgcgcgag accacggtca gtgcaaacaa gaaggccagg cgcaccgatg ccgaactgca
     1921 gggagtgagt cggaggcagt tcctatccaa ggcctgggta ggcggcatca tcgtggcatt
     1981 cgcgcagttc ggtctggcat ctctcgactt cctctacccc cgactccgcg gcggattcgg
     2041 cgccaagatc accgttggcg atgtcgagga gtaccgccag ctgatctctg ccgcgcgtca
     2101 gccggtattc atccccgatg ggaggttctg gctgacgatc ttcgagggcg atgccaagac
     2161 cgctgagaag gtcccgacct acaagctagc aaacgtccat gaaacggggt tcatggccct
     2221 ctatcgcaag tgtgtgcacc tcgggtgcag cgtgccctgg tgtgacagcg caaagttctt
     2281 tgaatgtccg tgccacggct cgaagtactc ggtcaacggc gagtggcgtg acggtccggc
     2341 ccccaggagc ctcgatcagt tccgcgtcga gatcgtcggc ggaaaactag ttgtcgacac
     2401 ctccagcgtc atagagggag ctcctcgcgg aacgatcacg tcgcagccgc agccagaggg
     2461 cgtgcactgc gtgaacgtct agtgctcgac tacgaaacga gaaagtagaa gatgagacag
     2521 gtagcgatcg cggttgtcat ccttggcttc ttggccatcg caatagccgt tctcacccgg
     2581 cgaggaccgc gcggcgccga gagagtggcc cccgctctgc gcccaggtcc gtcagatgac
     2641 gtcctcgagg acgagcgtct taccaagacc atgcggtggg gtgtggttat cgtcttattc
     2701 tttgcgatat tcctgccgat ttactggttc gccgagcctg gaaggatgga cgctaagacc
     2761 aaagcgttcg aagaagagtc catatctcgt ggcaaggcct actatgcgct tcgagtcgac
     2821 caggagactg gcgaggagaa tcacgagggc gtcgagtgcg ctcgctgcca cggtagcgaa
     2881 gcgcagggcg gcgagactca gttcctcgat cccaactcag gccaggtccg caacgtcaag
     2941 gttccgagtc tgttcgatgt gtttgcaaga tacgagacgg ccccgccggg ctttaaagat
     3001 gctcgcgcgt tcatcctcga gaccatagag cgcggccgac caggcaccgc catgccgacc
     3061 tggggcaacg aaaacggcgg ccccctcaca gagcagcaga tcgatgacat catcaactac
     3121 ctgtatgcga tccagcatgc gcccgaggtc aaggcacaag ccaccggcgc ggatatcttt
     3181 tcccagaact gctcgccctg tcatggcatc gggggcacgg gcggcgcggg ccccgctatg
     3241 gtgaatgggg cggtcgctgc tcagttcccc accatcgaag atcacatcgc cttcgtgaag
     3301 gcaggatcca agcagggcgt taagtacggc acgaatggca agggcaccgg cgggatgccc
     3361 gcgtggggca agctacttac cgatgcgcag atccaactgg ttgttgagta tgagaggagc
     3421 ctctagttgg acccagtctt cgtcaaaggg atcgtcgctt tcgctctgat gatcctcgtc
     3481 ttcatcggca gcgtctacct cctgctctcg atgatcctgg gggcccggct tggctacctc
     3541 gttctaggct cctgtttctt cggggtcatg atcctgatag cccttatctg gatggtgacc
     3601 gcccttgggc ccaagggtcc tgaaaccaca tgggtaactg tcaggacggg accctctctc
     3661 tcgaccatcg agtcggagtt cggcaacttc gacgtatcca gctacccaaa cggatgggtc
     3721 gacccaagcc cagatggcca cctcgccgac ctctcgggcg aggacaacac gcagacggag
     3781 ttggaaaacg tcaaacccgt cctctcgttg ctagtggacg aggcgatctc gcccatcccc
     3841 ggtatccgca aaagggttgc tgatcaagtt gatggttcga tctcactgga gacgggtcac
     3901 ttcgaggtta tcgacgtgag gatgaaggag gcaaaggtca acggcaagga ctcgatcatc
     3961 gccgttggta gggccgtatc gtcggcgaca ctgtctgccg tggagttctc gaatggggcc
     4021 aaggagggca cagtggcccg cttcctcgtc tcgaacggcg acgccgtagg ccagggcact
     4081 ccccttgttg aggtgacaac cgacacgggc accatcgcac tcgtatctcc caaggacggg
     4141 aagctcatcg cgttcggcct tcgtaaaggc gacaagatca agcctggcgt gcccttcgct
     4201 aaactcgatg tcacgggcca gccaggagcc gcggagcccg ctctggttgc cggggtgcga
     4261 gtcaggggcg ctgtgcggac aaccgcgctc tactacctgg ccgcctgcac ccttctattc
     4321 gctctgcaca tggcggccct gagccgatcc gaaaaggctc gcaagacagt gccacaaccc
     4381 gcctaacccc ccagatcaga cgggagctac caggaccggc gacggattga gcacttgaag
     4441 ctcaacttgc aaccggatcc gaaaatctca gcctgggaga ttctcggcag cccctgtgcg
     4501 catcctatgt tcgtagaagc tcaggatctg caattccatc gaaaggtcca cgtggcgtag
     4561 tg
//
LOCUS       DATDOZ010000071         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3452307, whole genome shotgun sequence.
ACCESSION   DATDOZ010000071 DATDOZ010000000
VERSION     DATDOZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3452307"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1076)
                     /gene="pheS"
                     /locus_tag="VI541_01370"
     CDS             complement(30..1076)
                     /gene="pheS"
                     /locus_tag="VI541_01370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLF69584.1"
                     /translation="MGSEVAQELKRIEKEALAAIAAAAASADLEDARVTSLGRRSRLA
                     EISRDLGSFDDATRRDVGRVINEVRGSIEGALAARLSELAKADLVERLEAERIDMTLP
                     GRAPARGAIHPLTTVIDDIVDVFVGLGFKVAEGPIVETDYYNFEVLNIPKDHAARSMH
                     DTLYVESSTPESILVKTHTSPMQARLMQSQSPPIYVVIPGRCARRDETDSNHLSSFMQ
                     IEGLAVDEGISFSDMKGTLEVFAKAMFGPQQRVRMHPSYFPFTEPSAEVYVSCFVCDG
                     SGCPTCRGEGWIEIMGAGMVHPNVLNAVGYDAERYTGFAFGMGVERIAKLRYGVHDLR
                     TFYENDLRFLGAFR"
     gene            complement(1106..>2032)
                     /locus_tag="VI541_01375"
     CDS             complement(1106..>2032)
                     /locus_tag="VI541_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69585.1"
                     /translation="GSRIDELIDTHDVNGNQCPIGASPRLERMPTVKATPEQEFLART
                     KSGEEVWVGVTCNFERDGASKITRTIAVMRDIRRRKLIDLAKSEVISAVSHELRSPLT
                     SVKGFTSTLLHKWDRFDDDTKKHLLLTINTDADRVTRLIGELLDVSRLEAGRLQLRRQ
                     MVPVADVAHRVVERIQPRSTEHALSSRFPDGFPEVFCDPDKVEQVLTNLVENAVKYTE
                     SGEVVVTGVVEESVVKISVTDHGPGIPPEQRAQVFGKFFRRGETARAENPTGTGLGLY
                     ISKGLVEAHGGRIWIEDPDHRGACFAFTLPRS"
BASE COUNT          397 a          631 c          585 g          419 t
ORIGIN      
        1 gaactcgctc aaccatgaga gcggaaccct catcggaacg ccccgaggaa tcgaagatcg
       61 ttctcgtaga aggttcgcag gtcgtgcacg ccgtatcgaa gtttcgctat gcgctccaca
      121 cccatgccga aggcaaagcc cgtgtatctc tcggcgtcat aacccacagc attcaacaca
      181 ttggggtgca ccatgcccgc ccccatgatc tcgatccaac cctcccccct gcatgtcggg
      241 cacccgcttc cgtcacagac gaaacatgaa acgtatacct ccgccgaggg ttcggtgaat
      301 gggaagtagc tcggatgcat cctcacacgc tgctgggggc cgaacatcgc ctttgcgaag
      361 acctcgaggg tacccttcat gtcggagaag gagatcccct cgtcgacggc gagtccctct
      421 atctgcatga aggacgagag atgattcgag tcggtctcgt cgcggcgggc acatctgcct
      481 gggatcacca cgtagatcgg tggcgattgc gactgcatca gccgggcctg catcggagac
      541 gtatgtgtct tgacaaggat cgactctggc gtcgacgact cgacgtacag agtgtcgtgc
      601 atcgagcgtg cagcgtggtc tttggggatg ttcaggacct cgaagttgta gtaatcggtc
      661 tcgacgatgg ggccctcggc gaccttgaac cctaggccga cgaagacatc cactatgtcg
      721 tcgatgaccg tagtgagggg gtgtatcgct ccgcgtgcgg gggcccggcc ggggagggtc
      781 atatcgatcc gctcggcttc gagccgttca accagatcgg ccttcgccag ctcggagagc
      841 ctggcagcga gtgctccctc tatggatcca cgaacctcgt tgataacgcg accgacatcg
      901 cgacgtgttg cgtcgtcgaa ggaaccgaga tctctgctta tctcagcaag acggcttcgg
      961 cgccccaaag atgtgacccg cgcatcctcg aggtcggctg aggcagccgc cgctgcgatg
     1021 gcagcgagtg cctctttctc gatccgtttg agttcttgcg cgacttccga gcccatcgcc
     1081 ccccaggtcc atcagatgct gatgcctatg aacgcggaag tgtaaacgca aagcaggcgc
     1141 cgcgatggtc agggtcctcg atccagatcc tgcccccatg cgcctcgacg agcccttttg
     1201 agatgtagag gccaaggccg gtccctgtgg gattctccgc cctggcggtc tcaccccggc
     1261 ggaagaactt tccgaacacc tgtgctcgct gctccggggg tatgccgggg ccatgatcgg
     1321 tgacggatat cttaacgact gactcctcga cgaccccggt cacgacgacc tctccagatt
     1381 ccgtgtactt caccgcgttc tcgacgagat tcgttaaaac ctgttcgacc ttgtctggat
     1441 cgcagaacac ctctgggaat ccatcgggga atcgagaact caatgcatgt tccgtcgaac
     1501 gtggctggat cctctccacg accctgtgcg cgacatcggc caccgggacc atctgacgtc
     1561 gcaactggag cctgcccgcc tcgagccgcg agacgtccag aagctcgccg atcaaccgag
     1621 tgacgcggtc ggcatccgta ttgatagtga gaagcaagtg tttcttcgtg tcatcgtcga
     1681 agcgatccca cttgtgaagc agcgttgaag tgaagccctt caccgacgtc aagggcgaac
     1741 gcagttcgtg agacaccgcg gagatgactt cggacttcgc aagatcgatc agtttgcggc
     1801 gcctgatgtc tcgcatcacc gcgatcgtgc gcgtgatctt cgacgcacca tctctctcga
     1861 agttacacgt aacaccaacc cagacctctt cgccgctctt agtccgtgcg aggaactcct
     1921 gctccggtgt cgctttgacc gttggcatgc gctcaagacg cggggaggct ccgatggggc
     1981 actggtttcc attgacgtca tgggtgtcga tgagctcgtc tatccggctc cc
//
LOCUS       DATDOZ010000072         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_837062, whole genome shotgun sequence.
ACCESSION   DATDOZ010000072 DATDOZ010000000
VERSION     DATDOZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837062"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VI541_01380"
     CDS             complement(<1..566)
                     /locus_tag="VI541_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLF69586.1"
                     /translation="MSILVQGLALGAIYGLLAIGLVLVFKATRVINFAQGEFGTIGAY
                     LAWFLIMRARLPWGVGLLISAALVGGIGYMWERFVIRRMLDGPKLTMAVATLGLMILL
                     GFIGIRFGPRILRQPFAGRGPELFGTLIPPARLLAIAMAIVVGGGLYLFMKRTTFGLG
                     LLAAAQDPIAVRLMGIRLRHLSAFTWIVA"
     gene            complement(563..1174)
                     /locus_tag="VI541_01385"
     CDS             complement(563..1174)
                     /locus_tag="VI541_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLF69587.1"
                     /translation="MGVTQDSKELAPLTPKGEATRKRLLVASEDVFGEVGFDQASVSE
                     ITRRGGVAQGTFYLYFPSKRAVFSEVVRSIGRDVRHVIQEAVAGLDDRLEIERKGFET
                     FFAYVRKHPDIYRIVRQAEFVDRDAFRDYYESFADGYVKGLKHAARKGEFRDLDPEVV
                     AWCLMGMGDLLGIRWILLRDEGKVPDRVLDTMIELIGNGLAAR"
     gene            1541..1993
                     /locus_tag="VI541_01390"
     CDS             1541..1993
                     /locus_tag="VI541_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLF69588.1"
                     /translation="MPELRSSLPSDPSPPGGSSQSVTWPEGSEDGDYARAIAVEYQSE
                     LLGEIAVAKGRGEQITKQDDALLADLASQVGLAMRHLRLTEELRHRLIELQDSRKRIV
                     AASDTQRRRMERDLHDGAQQQLVSMSVKLSLLRGLISRDQTKAEAMAG"
     gene            complement(2015..2230)
                     /locus_tag="VI541_01395"
     CDS             complement(2015..2230)
                     /locus_tag="VI541_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLF69589.1"
                     /translation="MRDYHINVFWSEEDSQWIADIPDLKGCSASGDSPEEAVKEVQVA
                     KKLWLEVAEQDGEKIPQPNYRPAIYAT"
     gene            complement(2233..2487)
                     /locus_tag="VI541_01400"
     CDS             complement(2233..2487)
                     /locus_tag="VI541_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLF69590.1"
                     /translation="MKPQRLLRRIQQGEVNNIRFSDFVRLVEDLGFVLRRTEGSHRIY
                     KHPDLAEKLNLQPLTNGDVKPYQARQLVRLVEQYSLSLEV"
     gene            2620..>3403
                     /locus_tag="VI541_01405"
     CDS             2620..>3403
                     /locus_tag="VI541_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69591.1"
                     /translation="MRKLHVLSAVVALALLLVACSGYPAARLQSKGDDTIEIIEPLPS
                     QSPVEALEGGTTPSPGTTGSPRPKQSPRVIARTKSGTTITVGPGGLPRADIWPAADDR
                     RGFTDNEIKLCMHAAFALGPVFNTSPDDGNVYWKKVNAEGGVFGRQVKVTYLDDLYTS
                     AGTTQALDKCESEQDPFLYIGGVGFDQAPAGRSWAESHHQPYLFNMAVETTGLKYSFS
                     VLPSIETNGRILGQFVGSRFPGKKVAVIYVDTDNWRAGYQTFE"
BASE COUNT          669 a         1069 c         1011 g          654 t
ORIGIN      
        1 gccactatcc aggtgaaggc agagagatgg cgcagccgga tgcccatcag tctcaccgct
       61 atgggatctt gcgcggcggc tagtagtccg agcccaaatg ttgtgcgctt catgaacagg
      121 tagagacccc cgccgacgac gatcgccatc gcgatagcga gcagtcgcgc gggaggtatc
      181 aaggtcccga agagttcagg acctctgccc gcgaagggct ggcggagtat ccgggggccg
      241 aagcggatgc cgatgaatcc gagcaggatc atcaggccga gtgtcgcaac agccatcgtc
      301 agtttcgggc catcgagcat ccgtcggatc acgaaccgct cccacatgta tccgatgcca
      361 ccgacgagcg cggccgatat caataggccg actccccagg gaagtcgcgc gcgcatgatg
      421 aggaaccatg cgaggtaggc gccgatcgtc ccgaactcac cctgcgcgaa gttgatgacg
      481 cgtgtggcct tgaacacaag gaccagaccg atcgccagca agccatagat cgcgccgagt
      541 gcgagaccct gaacgaggat gctcaacgcg ccgccaagcc gttgccgatg agttcgatca
      601 tggtgtcgag cacgcgatcg gggaccttgc cttcatcgcg aagcaagatc cagcggatgc
      661 cgagcaggtc gcccatcccc atgagacacc acgcgacgac ctcggggtcg aggtcccgaa
      721 actcgccctt gcgcgcggca tgcttcaggc ccttcacgta cccatccgcg aaagattcgt
      781 agtagtcgcg aaacgcatct cggtccacga actctgcctg gcgcacgatc cggtagatgt
      841 cggggtgctt ccgaacgtat gcgaagaatg tctcgaatcc cttgcgctcg atctcgaggc
      901 gatcgtcgag tccagccacc gcttcctgga tgacatgccg aacatctcgg ccgatggatc
      961 tgaccacttc ggagaagacc gcgcgtttgg acgggaagta gaggtagaag gttccctgcg
     1021 ctactccccc ccgccgcgtt atctccgaga ccgacgcctg gtcgaatccg acttcgccga
     1081 agacatcttc cgatgcgaca agcagtcgct ttcgagtggc ctctcccttg ggggtcaggg
     1141 gggccaactc cttcgaatcc tgcgtaacgc ccatacacgg attctgtcag aaaactgaca
     1201 cctgactcag gtcccctcct accaacactc gtcggccccc ggagcctcga tccgtcggac
     1261 cgtcgccagt catctcagca tgcggggagt gttcgagcag tcgaaccgcg gcccagtcat
     1321 gatccatctt gccgatggtc ggtgcagatc agaaccctgg gcgtagtcga gctagccctt
     1381 tgggcgatca ccctaccctc acggggggca ggtgagcggc cgggtattga tgacctgctt
     1441 ctgctcgtag cgttctcaac cttcgggact gcgggcgcgc tcatcgcatc gaaacgtccc
     1501 aagaacccct tgggctggct catgatcgca gcctctttct ttgccggagc ttcggtcctc
     1561 gctcccgagt gacccttcac ctcccggcgg ttcatcgcag tcggtcacct ggcccgaggg
     1621 atccgaggat ggggactacg cccgagcgat agctgtggag taccagtcgg aactcctggg
     1681 agagatcgcg gtcgccaagg gtcgcggcga gcagataacc aagcaggacg acgctcttct
     1741 ggccgacctc gcatctcaag taggcctcgc gatgcgccat cttcgattga ccgaggagct
     1801 tcgtcaccgt ctcatcgaac ttcaggactc ccgaaagcgc atcgtcgccg catccgacac
     1861 ccagagacgc cgcatggagc gagacctcca cgacggcgca caacagcaac tcgtctcgat
     1921 gtcggtgaag ctctcgcttc tgcggggcct catctcgcgc gaccagacca aggccgaggc
     1981 catggcaggt tagccccgag tcatgagggg tgaatcaggt ggcgtagatg gctgggcgat
     2041 aattgggttg ggggatcttc tcgccatcct gctcagcaac ttctaaccac agcttcttgg
     2101 ccacttgcac ttccttcacc gcctcctcgg gcgagtcgcc cgaagcggaa catcccttga
     2161 ggtcggggat gtcggcgatc cactgggagt cctcttcgct ccagaaaacg ttgatgtggt
     2221 agtcgcgcat gatcaaacct ccagactcaa agaatactgc tccaccagcc ggacgagttg
     2281 gcgggcctgg tatggcttga cgtctccatt tgtcagcggt tgaaggttga gtttctcggc
     2341 tagatccggg tgcttgtaga tgcgatggct cccctcggtt ctgcgaagta cgaacccgag
     2401 gtcctcgacg agccggacaa agtcggagaa gcggatgttg ttgacctctc cctgttggat
     2461 ccgtctcaag agtctctgtg gtttcacttg tgtttccccc tcggcacttc gaagatgagc
     2521 ctaagagggg tgtatgacat cgcgggttct agcgaggccc ccaagtggag ttcttcggag
     2581 taaactgacg agtgagtcag gttggtggag ggttcgcgat tgagaaagtt gcatgtcctc
     2641 tcggccgtag tggccctagc gttgctcttg gtcgcgtgca gtggctaccc ggcggcccgg
     2701 ctgcagagca aaggcgatga cacgatcgag atcatcgaac cgctccccag ccagtctccg
     2761 gtcgaggctc tcgagggcgg caccacacca tcaccaggta cgacaggctc cccgcggccc
     2821 aagcagtcgc ctcgcgtcat agcgcgcacc aagtcgggga ccacgatcac ggttggaccc
     2881 ggtggacttc ctcgcgctga tatctggccg gccgccgacg atcgacgcgg cttcaccgac
     2941 aacgagatca agctctgcat gcacgccgcc ttcgcgctgg gcccggtctt caacacaagc
     3001 cccgatgacg gcaatgtcta ctggaagaag gtgaacgccg agggcggtgt cttcgggcgt
     3061 caggtgaagg tcacctacct cgatgacctc tacacctccg cgggcaccac tcaggccctc
     3121 gacaagtgcg agtcagagca ggatcccttc ctatatatag gtggagtcgg attcgaccag
     3181 gcccccgcag ggcgttcgtg ggccgagagt caccaccagc cctatctctt caacatggcg
     3241 gtcgagacca cgggactcaa gtactccttc tcggtgcttc cttcgatcga gaccaatggc
     3301 cgcatcctcg gacagttcgt cgggtctcgc ttccctggca agaaggttgc cgtcatctat
     3361 gtcgacaccg acaattggcg tgcgggttat cagaccttcg agg
//
LOCUS       DATDOZ010000073         3848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_575401, whole genome shotgun sequence.
ACCESSION   DATDOZ010000073 DATDOZ010000000
VERSION     DATDOZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575401"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VI541_01410"
     CDS             <1..436
                     /locus_tag="VI541_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLF69592.1"
                     /translation="EFGGIIRHGAKLLYAFTEATVPRLTVVTRKAYGGAYVVMNSKHI
                     RADINFAWPTAEFAVMGAAPAVNLLHRKELSDARNPAALRSQLVSEYEERFLNPFIVA
                     ERGYIDDVIEPPQTRTRLIQALEMLQTKRETLPQRKHGNIPL"
     gene            complement(614..1816)
                     /locus_tag="VI541_01415"
     CDS             complement(614..1816)
                     /locus_tag="VI541_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69593.1"
                     /translation="MIRRRIAMFVAVAIFGVPALAIALPHPIGTPQVDRTLLQTLTLP
                     ASGAIGSSGLLTADVLEQSRAYIFEVTGTWRPDKNTLPNKAADAAYYTTNKDSNPWSP
                     PARSQNGSGFMAFNTKFGESAQQVAFKANHVYSFNWVGRGVRESLRVFDPDSDWDDND
                     GSLTVSVFKVNKVTYTYFLQQGIPPEPVPAPYVVQTIPTTTVAPGVVPGVTVTAPVPG
                     VEIPTVAYVNHNHNDPSFPGLNCLSITIPLTTPPITTQIGCVYDAGLIPDGSSAIGGG
                     QVISPTTICNPCPSVDGVTPTAVVPSTSIEGGVPPGSKLDLTITWSADPSRLYPFYRN
                     GSSEVWAPFDPVVDGPWFATNAASVAATVRAVIRDSSGAVIYDVPITLPGMGQVIEGI
                     FASKVVGI"
     gene            complement(1931..3289)
                     /locus_tag="VI541_01420"
     CDS             complement(1931..3289)
                     /locus_tag="VI541_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69594.1"
                     /translation="MNRRRMILFTVLAMLAPQFAHAVPRDATSPEGEALFHQTQPETP
                     ITSVNVAANSSEGVTLTLTPGRRYTFYVSGYWCPSSTTCPASTPNQADAGFETTNAWS
                     THFQSNGRGIIIDGANYGLPAEDLNFSPGHRYVMFVAAPVTGPTPGQVRVRIADGNYS
                     NNSGSLKVDVTSQTKVAWNLTYSVDVGTSLPSPPDRTPEIGVDGRPYMSTQTVTNVAV
                     PAITVPVNVPLIAEVQIEAQTTSDPNCARRIIFHVKLMGQEAPGSPRELACVPANIGP
                     FPFGDSLRQYHPVGGSTSTTGTPPIDVCATFATATFGQWNNTDPNNPNLQPRPEGPNC
                     VIPPPHSVYIGSSQINIPIPNPLPPGSKLQIEVKWKADTSKLFLLYRDLAGDEVWAPF
                     GPQDAQWFQDNANDLDVNVVGTVLGPGGEAFFKVESKTQAPMPGTGQILEALFASKIR
                     GS"
     gene            complement(3454..>3848)
                     /locus_tag="VI541_01425"
     CDS             complement(3454..>3848)
                     /locus_tag="VI541_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69595.1"
                     /translation="LVRTVATSDQVLADSKALVLAVGKHPVFCEDRAGFIVNRLLFPY
                     LNDAVRMLEQGYALAEEIDDAMRLGCGHPMGPLALIDLVGLDVTLEILRSLHEEFRDE
                     AFAPAPLLEYLVKAGYLGKKTKQGFFAY"
BASE COUNT          762 a         1138 c         1221 g          727 t
ORIGIN      
        1 agagttcggt gggatcatcc gccacggggc gaaacttctc tacgcattca ccgaggccac
       61 ggtcccccgg ctcacggtcg tcacgcgcaa agcatatgga ggggcatacg tggtcatgaa
      121 ctccaagcac atccgagccg acatcaactt cgcctggccc actgccgagt tcgcggtcat
      181 gggggccgca cccgcagtga accttctgca tcgcaaagaa ctcagcgatg ctcgcaaccc
      241 tgcggctctg cgttcacagc tcgtctccga gtacgaggag cgcttcctga acccgttcat
      301 cgtcgcggag cgcggctata tcgacgatgt gatcgagccc ccgcagaccc gaactcgtct
      361 catccaagca cttgagatgc tccagaccaa acgcgagacc ctgccccagc gtaagcacgg
      421 caacatcccg ctgtgaccag gagcatccgg atcatcacag gttctcccac ggctcaggag
      481 cagatggcca taagggcggc aacagagttg ctccagcgat ctcagatgtc ccctgacgac
      541 ccggccccag ataggtggcg atcgaggaaa cctatcctcg aagccaaggc ccccctacga
      601 aggagttcct gggtcagatc ccaactacct tagaagcgaa gatcccctcg atgacctgcc
      661 ccatgccggg cagagtgatg ggaacgtcgt agatcacggc accgctcgag tcgcggatca
      721 ccgctcgcac ggtcgccgcc accgaagcgg cgttcgtcgc gaaccacggt ccatcgacta
      781 ccgggtcgaa tggggcccaa acctcgctgg aaccgttgcg gtagaagggg tagagacggc
      841 tcgggtctgc cgaccacgtg atggtgagat cgagtttcga acccggcggc acgccgccct
      901 cgatgctcgt gctcggcacc accgcggtcg gcgttactcc gtcgacgcta gggcatgggt
      961 tacagatcgt tgttgggctg atgacctgcc cgccgcctat cgcagagctg ccgtcgggta
     1021 tgaggccggc atcgtagacg cagccgatct gggtggtgat cgggggcgtg gttagcggga
     1081 tggtgatcga caagcagttg aggccgggga acgaagggtc gttgtggttg tggttcacat
     1141 aagcgacggt cgggatctcg actcccggca cgggagcagt caccgtgact cctggaacca
     1201 caccaggggc gaccgtggtc gttgggatgg tctgaaccac atagggagcg ggcaccggct
     1261 ccggcgggat gccctgctgc aggaagtagg tgtaggtgac cttgttcacc ttgaagacgc
     1321 tcaccgtgag ggagccgtcg ttgtcatccc agtcgctatc cgggtcgaag accctcagcg
     1381 actcgcgcac accccggccc acccagttga acgagtagac gtgattggcc ttgaaagcaa
     1441 cctgctgtgc ggactcaccg aatttcgtgt tgaaggccat gaagccgctg ccattctgcg
     1501 agcgcgccgg tggagaccag gggttgctgt ctttgttcgt tgtgtagtaa gcagcgtcgg
     1561 ctgccttatt cgggagggtg ttcttgtcgg gcctccacgt gccggtgacc tcgaagatgt
     1621 acgcgcggct ctgttcgaga acatcggctg tcagcagtcc cgaagatccg atggcacccg
     1681 acgcgggcag cgtcaaagtc tgaagcagag tgcggtcgac ctgtggcgtg ccgatggggt
     1741 gcggcagagc gatcgctaac gcgggcactc cgaagatcgc aaccgctacg aacatggcga
     1801 tccggcgacg gatcattggg gcttcctttc cctctcttgc ggaaactgcg tcactatgag
     1861 ttcgaccttt cgtccttgaa tcctgccaac accaacccac ttgggacgaa atacgccatc
     1921 gagcgtagcc ctaacttcct cggatcttcg atgcgaatag cgcctcgagg atctgacctg
     1981 ttccgggcat cggcgcctgg gtcttcgact caaccttgaa gaacgcctcg ccacctgggc
     2041 ccaggacggt accgacaaca ttgacatcga ggtcattggc gttgtcctgg aaccactgcg
     2101 cgtcctgggg gccgaagggg gcccagacct catcacccgc gaggtccctg tagagcagga
     2161 agagcttcga ggtgtcggct ttccacttga cctcgatctg caacttcgaa cctgggggca
     2221 gtgggttggg gatggggatg tttatctggg aggacccgat atagacgctg tgaggaggcg
     2281 gtatgacgca gttgggcccc tcaggcctag gctgcaggtt ggggttattg gggtcagtgt
     2341 tgttccattg gccgaaagtg gccgtagcga aggtggcgca aacgtcgatg gggggagtac
     2401 cggtcgtgct ggtcgagcca cctaccggat ggtactgcct gagcgagtcc ccaaatggaa
     2461 agggcccgat gttcgccggg acgcaagcca gttctctggg agacccagga gcctcctgcc
     2521 ccatcagttt tacgtggaag attatccgtc gagcgcagtt cgggtccgag gtggtttgag
     2581 cctcgatctg aacctcggcg ataagaggaa cgttgaccgg tacagtaatc gcgggcacag
     2641 ccacattggt gacggtctga gtggacatgt agggacgacc atctaccccg atctccggcg
     2701 ttctgtccgg aggagaagga agagacgtgc cgacatcgac cgaataagta aggttccacg
     2761 cgaccttggt ctggctcgtg acatcgacct tgaggctccc gctgttgttc gagtagttcc
     2821 catcggcgat gcgcactcgc acctgaccag gggtcggtcc cgtgacggga gccgcaacga
     2881 acatcacata acggtgccct gggctgaagt tgaggtcttc agcaggcaga ccgtagttgg
     2941 cgccatcgat gatgattccc cttccgttgc tctggaagtg ggtcgaccag gcgttagttg
     3001 tctcgaaccc ggcatcggct tggttcgggg ttgaggccgg gcatgttgtt gacgaggggc
     3061 accagtaccc actcacgtag aaggtgtagc ggcggccggg cgtaagagta agggtcacgc
     3121 cttccgagga gttggccgct acattgacac tggtgatcgg ggtctcgggc tgtgtctggt
     3181 gaaagagcgc ttcaccctcc ggagacgttg cgtcgcgagg caccgcatga gcaaactgag
     3241 gcgcgagcat cgcaagtacg gtgaacagga tcatgcgtct gcggttcact ctgtctcctt
     3301 ccgcccggag taaacttccc ggcactctca ggacttcgcc gtcagccccg cgaatcctcc
     3361 cggaaaaagg ccgccaaggt gcacaggcgg gagtttacgc ccaatgacgt agggcccccg
     3421 gaaaaacggg ctcatcagag gcaaccttag cgctcagtag gcgaagaatc cctgcttggt
     3481 cttcttcccc aggtatccgg ccttgacgag atactcgagc aggggggccg gtgcgaaggc
     3541 ctcgtcccga aactcctcgt gaagcgaccg gaggatctcg agcgttacgt cgaggcccac
     3601 gaggtcgatg agcgccaggg ggcccatcgg gtgaccacaa ccgaggcgca tcgcatcgtc
     3661 gatctcctcg gccaacgcat atccctgctc cagcatccgg acggcatcgt tgaggtaggg
     3721 gaagagaagt cggttcacga tgaagcccgc acggtcctca cagaagacgg gatgcttgcc
     3781 gacggccaga acgagcgcct tcgaatcggc cagaacctgg tcagacgttg cgacggtccg
     3841 gaccaact
//
LOCUS       DATDOZ010000074         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2670148, whole genome shotgun sequence.
ACCESSION   DATDOZ010000074 DATDOZ010000000
VERSION     DATDOZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670148"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VI541_01430"
     CDS             complement(<1..256)
                     /locus_tag="VI541_01430"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935791.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HLF69596.1"
                     /translation="MTSPSSQSIGGYFGEFGGRFAPEALIASLEELAEEMDRAFADED
                     FLSELDRLNREISGRPTPLYLAERLTAHAGGARIWLKREDA"
     gene            complement(276..1046)
                     /gene="trpC"
                     /locus_tag="VI541_01435"
     CDS             complement(276..1046)
                     /gene="trpC"
                     /locus_tag="VI541_01435"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HLF69597.1"
                     /translation="MNSKLEAIIEATHDRVASAKRARPESSMEPTGWRRDFEGALRLD
                     GISLIAEFKRASPSAGFIAQDAHPAAVAMQYEEGGARALSILTEPYFFKGDLAHLTLA
                     RGACSLPVLRKDFIIDPYQVPESASVGADAVLLIVAAVEVSLLHDLFDSARESGLDAL
                     VEIHDERELETALGLEPAIIGINQRNLHTFQVDTSLAARLRELVPAAVVVVAESGIKL
                     SSDLGVLEDAGIDAVLVGETLMRVPDRLAAVRSLIGDR"
     gene            complement(1043..1792)
                     /locus_tag="VI541_01440"
     CDS             complement(1043..1792)
                     /locus_tag="VI541_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLF69598.1"
                     /translation="MIDIARELSVGIGERVKGTDGERRAADIIEMHFDAAGCQTSRQT
                     FTRAAGGTSQNVICRVPEADFSHGWTVIGGHYDTVPGSPGGNDNASGSALTVALAEAF
                     VRSRAPIEFVAFSSEERDRATRAHHEGSRAYVAQLSAPGMVLAMISPDMIANGPRLHI
                     TIDRSHPGQLGAELLTLADELKIPAFIDRAGDVSDHTSFTRVGVPAVALWTGRRPEYH
                     SAGDTFDTVDRASLMMSITLMDSWLRRRYGL"
     gene            complement(1939..>2785)
                     /locus_tag="VI541_01445"
     CDS             complement(1939..>2785)
                     /locus_tag="VI541_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF69599.1"
                     /translation="VASFVARRITSPLRDLAAAAADVATGDFAARVEVSSDDEVGVVA
                     ASFNKMASELGDADRLQREFFLSISHELRTPLTAIQGYAEAIEDGTVSGQQGKEAAGV
                     IIGESKRLNRLVSDVLDLARLDAKRFTVHLEELDVRTVLQRLQQAFAQKASESGVAVE
                     IEAADMTVKADSDRLIQVLSNLVENGMRYTPTDGRIRVSATPEGSRCRFDVVDGGPGL
                     DPADFRRAFDRQYLWGKYRGVRDVGTGLGLAITKELVEAMGGEVSSTNDAQGGARFSV
                     LLPLA"
BASE COUNT          555 a          900 c          819 g          511 t
ORIGIN      
        1 cggcgtcctc gcgcttgagc catatgcgag cccctcccgc atgtgcggtg agtcgttccg
       61 ccagatagag aggagttggc cttcccgaga tctcgcggtt gagtcgatcg agttcggaga
      121 ggaagtcctc atcagcgaag gcccgatcca tctcttcggc caactcttcg agcgacgcga
      181 tcagggcctc gggcgcgaag cgacccccga actcaccgaa gtaaccaccg atactctgtg
      241 aagaagggga agtcatggtg tcgcagtcta gacggtcacc ggtcaccgat cagtgagcgc
      301 accgcagcaa gtcggtcggg tacgcgcatc agggtctcgc ccaccagaac cgcgtcgatg
      361 cccgcgtctt ccaggacccc aaggtctgag ctcagcttga taccgctctc ggcgacaaca
      421 acgaccgcag ccgggaccag ctcccgcaga cgcgcggcca atgatgtatc gacctggaaa
      481 gtgtgaaggt ttcgttgatt gatgccgatg atcgctggct ccaaacccag agcagtctcc
      541 agctcgcgtt cgtcgtggat ctcaacgaga gcgtcgagcc ccgactctct cgctgaatcg
      601 aagagatcat gcagcagcga cacctccacc gccgcgacaa tgagtaggac cgcgtcggcg
      661 ccgaccgaag cactctcggg gacttgatac ggatcgatga tgaagtcctt gcgaagaaca
      721 gggaggctgc aggccccacg ggctagggtc agatgcgcca gatccccttt gaagaaatat
      781 ggttccgtaa ggatcgaaag ggctctggct cccccctctt catactgcat cgccaccgcg
      841 gcgggatgcg catcctgcgc gatgaacccg gccgatggtg aggcccgttt gaactcggcg
      901 atcagcgaga tcccatccag acgcagggct ccctcgaagt cccgcctcca acccgtgggc
      961 tccatcgatg attcgggtct ggcgcgcttc gcactcgcca cgcggtcatg cgtcgcctcg
     1021 atgatcgctt cgagcttcga gttcacaggc catacctgcg gcggagccag gaatccatga
     1081 gagtgatcga catcatcagg gatgctcggt cgacggtgtc gaaggtatct cccgctgagt
     1141 gatattccgg tcgcctgccc gtccacagcg cgaccgccgg gacccctacc cgcgtgaacg
     1201 acgtgtggtc cgaaacatca ccagctctat cgatgaacgc cgggatcttg agctcgtcgg
     1261 ccagcgtgag aagttcagcc cccagctgtc ccggatgtga gcggtcgatt gtgatgtgca
     1321 atcgtggacc attcgcgatc atgtcggggg agatcatcgc cagaaccata ccgggcgccg
     1381 agagttgggc aacataggct cgtgacccct catggtgagc gcgggtagca cggtcacgct
     1441 cttcgctcga gaaggcaacg aactcgatcg gggcccgcga acggacgaac gcttcggcca
     1501 gcgctacggt cagggccgag cccgaggcat tgtcattccc tccgggtgat cctggaaccg
     1561 tgtcgtagtg cccgccgata accgtccacc cgtggctgaa atcagcctct gggaccctgc
     1621 agatcacgtt ctgagatgtc ccgccggcgg ctcgggtgaa ggtctgcctc gatgtctgac
     1681 atccggctgc atcgaagtgc atctcgatga tgtctgccgc tcgcctctct ccatcggtgc
     1741 ccttcacccg ctctccgatg ccaaccgata gttcacgggc gatatcgatc acccgatcga
     1801 ctgagacggc tgtggcctgg ggcacaggac tcggtgaagg cgatggactc ggggaggtga
     1861 cacggacagg cggggcgccg actggtcgcg agcacccgga aagcaacagg ggaagaaccg
     1921 cgaccagccc cactgctctc aggccagcgg taggagtacc gagaatctcg cacccccttg
     1981 ggcatcattg gtcgaggaga cctcgccacc catcgcctcg acgagttcct ttgtgatcgc
     2041 cagccccaaa ccagttccga catcacgaac acctcggtac ttaccccaca gatactgtct
     2101 gtcgaacgcg cggcggaagt ctgcaggatc cagcccaggc cccccatcga cgacatcgaa
     2161 tcgacacctt gacccctcag gggtcgcgct caccctgata cgtccatccg ttggcgtgta
     2221 tcgcatcccg ttctcgacaa gattggatag gacctggatc aatcggtcgc tatcagcctt
     2281 gactgtcatg tcggcggctt cgatctcgac tgccacgcca gattccgatg ccttctgcgc
     2341 gaaggcctgc tgcagccgct gcagaaccgt tcgaacatcg agttcttcca gatggaccgt
     2401 gaaccgtttg gcatcaagac gggccaggtc gagcacgtcc gaaaccaaac ggttcaggcg
     2461 cttggactcg ccgatgatca ctcccgccgc ttccttgccc tgctgacctg agacggtccc
     2521 atcttcgatg gcctcggcgt acccctggat cgcggtcaat ggggtacgca gttcatgcga
     2581 gatcgagagg aagaactcac gttgcagccg atcggcgtcg ccgagctcag atgccatctt
     2641 gttgaaggat gccgccacca cgccgacttc gtcatccgag gaaacctcaa cccttgccgc
     2701 gaaatcgccc gtcgcgacat cggcagctgc cgcggcgaga tcacgaaggg gactcgtgat
     2761 ccgtcgagcc acgaatgatg ctacg
//
LOCUS       DATDOZ010000075         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3207213, whole genome shotgun sequence.
ACCESSION   DATDOZ010000075 DATDOZ010000000
VERSION     DATDOZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207213"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VI541_01450"
     CDS             <1..430
                     /locus_tag="VI541_01450"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLF69600.1"
                     /translation="ASFDAADTVVASLSVMKGLLETLSLDTDRMEAAARTGSSGATDL
                     AEALVRKGVPFREAHEVVGKLVARLGAEGRDLISATAEDLSSAHEAFDSEMLSLLDPR
                     SSVASRTSHGGTAPERIAEQFAALEATFDDEERWLGAYGA"
     gene            complement(449..694)
                     /locus_tag="VI541_01455"
     CDS             complement(449..694)
                     /locus_tag="VI541_01455"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HLF69601.1"
                     /translation="MSYRREQGRAVVVATPASPAEAELIKITLAFHGVEAMVSATFSA
                     YPSVDFVEGVRVLVSPEDEEKARDLLEQLRKKRDPGA"
     gene            779..>2138
                     /locus_tag="VI541_01460"
     CDS             779..>2138
                     /locus_tag="VI541_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLF69602.1"
                     /translation="MRESTPGMTNEEIASAFHELADLLELAGDERFKILAYRRAAEEI
                     NALATDAGAMTQKELQALRGIGKATAAKVVELITTGRIAKLEEARAAIPAGVREMMDL
                     PGLGPRKAMALHTELGIDSLEDLRRAVESQSLRAVPGFGPRTEESLGRALKRTAASRG
                     RLTLDKALAIAESLIEEMKRVPSVERAEYAGSLRRMRETIGDIDLLVATTDPGVAMRA
                     FTASKHAEQVIAHGLTKSSILTKSGTQIDLRAVAPDEFGSALQYFTGSKDHNVRVREV
                     AVARGLKLSEYGVFRVGADERIASVTEEEVYAAIGLATPPPTMREDRGEVQLAIKGEL
                     PKLVEVSDLRGDLHMHTTRSDGKASLEEMARSAIALGYSYIAVTDHGLHVNVKTLSLE
                     DIETQRAEIDVLNKSFKGFRILHGVELNIGADGGLDYDDDVLKGFDVVVASVHSGLTG
                     QRK"
BASE COUNT          433 a          639 c          689 g          377 t
ORIGIN      
        1 agcctcgttc gatgctgccg ataccgtcgt cgcttcgctg tccgtgatga agggccttct
       61 cgaaacgctc agtctcgaca cggatcggat ggaggccgcc gcgcgaacag gttcgtcggg
      121 ggcgacggat ctggccgagg ccctcgttcg taaaggagtg cccttccgcg aggcgcatga
      181 ggtcgtgggc aagttggtcg cgcgattggg ggccgagggc agggacctga tctccgctac
      241 ggccgaagat ctcagcagcg cccatgaggc attcgactca gagatgctct cgttgctcga
      301 cccgcgctct tcggtagcat cacgaacctc acatggaggt acggcccccg aaaggatcgc
      361 cgagcagttc gccgcgctag aagccacatt cgacgacgag gagcgctggc tgggggccta
      421 cggagcctga aaggtggcct ccctccggtc aggcccccgg atcgcgcttc ttccgcagct
      481 gttcgagaag atcccgcgcc ttctcctcgt cctcaggact gaccagcacg cggactccct
      541 cgacgaaatc gaccgaaggg taggcggaga aggtagctga gaccatggcc tcgacaccgt
      601 ggaaggccag ggtgatcttg atcagctcgg cctccgccgg cgaagcaggc gtagcaacta
      661 cgactgcccg cccctgttca cgtcggtaac tcacgcttcc atcctcgcgc cgatctgaag
      721 gatgttgcca gattcgacaa cctcccgcga tctgcgtcca ttaagtaggc tcgcagatat
      781 gcgcgaaagc acccctggga tgaccaacga ggagatcgct tcggctttcc acgagctagc
      841 cgatctgctc gaactcgccg gcgatgagcg cttcaagatc cttgcttatc gcagagcggc
      901 agaggagatc aatgcccttg cgaccgacgc gggggccatg acgcagaaag agctccaggc
      961 gcttcgaggc atcggcaagg ctactgcggc gaaagtggtt gagctgatca ccaccggccg
     1021 gatcgcgaaa ctggaggagg cacgggcggc gatcccggca ggggtgcgcg agatgatgga
     1081 cctccccggc ctcggccccc gaaaggcgat ggctctccat accgagctcg gcatcgactc
     1141 gctcgaagac cttcgacgag cggtcgagtc ccagagtctg agggcagtgc ctggtttcgg
     1201 ccctcgaacc gaagagagcc tcgggcgggc cctgaagcga acggctgcat cgcgtgggcg
     1261 tctcacacta gacaaggctc tagcgatagc ggagtcgctc atcgaggaga tgaagcgggt
     1321 accgtcggtc gagagagctg agtacgccgg gagcctgagg cggatgcgcg agaccatagg
     1381 agacatcgac cttctcgtcg ccacgactga tcccggcgtc gcgatgcgcg cgttcactgc
     1441 atcgaagcat gccgagcagg tcatcgctca tggacttacc aagtcgagca tcctcaccaa
     1501 gtcgggcact cagatcgacc tccgagccgt agcgccggac gagttcggat ccgcactcca
     1561 gtacttcact ggttcgaagg accacaacgt gcgagtacgt gaggtcgcgg tcgctcgcgg
     1621 actgaagctc tcggagtatg gcgtcttccg ggttggtgcc gatgagcgga tcgcatctgt
     1681 aaccgaggag gaggtatatg ccgccatcgg gttggccact ccacctccga cgatgaggga
     1741 ggacagagga gaggtacagc tcgcgatcaa gggagagctc ccgaagctgg tcgaagtctc
     1801 agacctgcgc ggcgaccttc acatgcacac gacgcgctcg gatgggaaag cttcactcga
     1861 agagatggca cgctcagcga tagctctcgg ctactcctac atcgcggtta ccgaccatgg
     1921 gctccatgtc aatgtgaaga ctctgtctct ggaagacatc gagactcagc gagccgagat
     1981 cgatgtactg aacaagtcgt tcaaggggtt tcggatactg cacggggttg agctgaacat
     2041 cggtgcggat gggggccttg actacgacga tgatgttttg aagggattcg atgtcgtggt
     2101 ggcgagcgtg cactcaggcc tgaccgggca acgcaagc
//
LOCUS       DATDOZ010000076         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1884266, whole genome shotgun sequence.
ACCESSION   DATDOZ010000076 DATDOZ010000000
VERSION     DATDOZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884266"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VI541_01465"
     CDS             complement(<1..622)
                     /locus_tag="VI541_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HLF69603.1"
                     /translation="MPSLERGFTAADALNHVDEHAFEVGPGDLVGVEAEWLVFDRANP
                     GDSLDFEAVRTALFGFDLPDSGSISFEPGGQLEISTAPVVGPVSAVDAVIGGHKNIQD
                     RLDRSGLVLVPVGIDPLRTGSRVVDSPRYAAMEKRFDHDGPDGRVMMRSTAAVQVNVD
                     IAEDPALQWRVAHEVGPLLTAIFADSPIYEGHRTGWVSTRMRTWLSM"
     gene            709..1122
                     /locus_tag="VI541_01470"
     CDS             709..1122
                     /locus_tag="VI541_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLF69604.1"
                     /translation="MVAPKVSGRLAALRSVPLFEHLPDKALRRILKLAGELEAARGQV
                     LVQPRTAGSGLFVVEEGQVVVETPKQRFELGPGEFFGELSLLTDRERSARVRALSDVK
                     CLCISRADFSSMLHTEPELAVSMLQILAHRIIDRN"
     gene            complement(1106..1930)
                     /locus_tag="VI541_01475"
     CDS             complement(1106..1930)
                     /locus_tag="VI541_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HLF69605.1"
                     /translation="MTQAPFTLDPVERARLPLIAHIPHSGTHIPRTIRSELLLGDEDL
                     RAEVLRVTDHFTDRLFASAAEAGGVRFVNHVSRLVFDPERFRDDADEVMASRGAGAIY
                     STTSDGRNLRSITASRREEVLVGFFDPYASAMADAVDEMKRRFGCCLILDCHSFPRVP
                     LGWELHLEGARPQIDVGTDPFHSPADLVTALTDVCAGEGLVTSRDHPFTGCYVPMRHY
                     RRDQRTWSVMIEIRRDLYMDESTGAESKGFASTAQLVDQMIGRSVQWLEERINSGR"
     gene            complement(1927..>2600)
                     /locus_tag="VI541_01480"
     CDS             complement(1927..>2600)
                     /locus_tag="VI541_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HLF69606.1"
                     /translation="HQVGDGRKRRSGQERMNLAQSLFALGGAAALIDWIAVARTNKFL
                     EYVFKPAALLFLLLSATAAPDGPGKSWVIAALALSLIGDVALMLPADLFIAGLGSFLL
                     AHLAFIIGFASIAYHPRTVLIAIPLLVMVGVGVARPVFRALRQGDRPLMLPVGIYIAA
                     IIAMAATAIGTHDILLGMGALLFMASDSLIAHDRFVRSRTWQSLTIIATYHLAQMAFV
                     ASVIR"
BASE COUNT          536 a          758 c          818 g          488 t
ORIGIN      
        1 ccatagagag ccaggttcgc atcctggtcg agacccagcc cgtccggtgc ccttcgtaga
       61 taggggaatc ggcgaagatc gcggtcagga gtggccctac ctcatgggcg accctccact
      121 gaagcgccgg atcctcggcg atatccacgt tcacctggac cgcggccgta ctgcgcatca
      181 tcacgcgtcc gtcgggaccg tcatgatcga atcgcttctc catggcggcg tacctcggac
      241 tgtcgacgac gcgcgagccc gtcctcagcg ggtcgatgcc gacgggaacg agcaccaggc
      301 ccgatcgatc gaggcggtct tggatgttct tgtggccccc gatcacagca tcgacggcag
      361 aaactgggcc gacgacgggg gccgtactta tctcgagttg gccgcctggt tcgaatgaga
      421 tggagcctga atccggcagg tcgaagccga acagcgcggt tctgacagcc tcgaagtcaa
      481 gagagtcgcc ggggttggca cggtcgaaga cgagccattc ggcttcgacc ccgaccaggt
      541 caccagggcc gacttcgaag gcatgttcat cgacgtggtt gagtgcgtct gctgctgtga
      601 agccgcgttc aagcgagggc atcctgcttg caacgatact ccaactccag gcaccggtcg
      661 tcgccccccc cgctgaacag agtcggctga gattggtata aagggttcat ggttgctccc
      721 aaagtctcgg gccggctcgc cgctcttagg tccgtgccgc tcttcgaaca tcttccagac
      781 aaggctcttc gcaggatcct gaaactggcc ggcgaactcg aagccgcacg cggtcaggta
      841 ctcgtgcaac cgaggacagc aggttctgga ctcttcgtgg tcgaggaggg ccaggtcgtt
      901 gtcgaaactc caaagcaaag attcgagctc gggccgggcg agttcttcgg agaactctcg
      961 ttgctgacgg atcgcgaacg aagcgcgcgt gtgagggccc tctcggatgt taagtgtcta
     1021 tgtatcagtc gggcagactt ctcatcgatg ctacacacag agccagagtt ggccgtgtcg
     1081 atgcttcaga tactggccca tcggatcatc gaccggaatt gatgcgttct tctagccact
     1141 gaacagatct cccgatcatc tgatccacga gttgggccgt cgatgcgaat ccttttgact
     1201 cagctcccgt cgactcgtcc atgtacaggt ctctacggat ctcgatcatg acggaccagg
     1261 ttcgttggtc gcgtcggtag tgacgcatgg gcacgtaaca ccccgtgaaa gggtgatctc
     1321 gagacgtgac cagaccctct cctgcgcaga catctgtaag ggcggtcacg agatcagcgg
     1381 gcgagtggaa gggatccgtc ccgacgtcga tctgcggccg agcgccctcg aggtgcaact
     1441 cccagccaag gggtacgcga gggaatgagt ggcagtcgag tatgaggcaa cagccgaatc
     1501 tgcgtttcat ctcgtcgacc gcatcggcca tcgccgatgc ataggggtcg aagaatccga
     1561 cgaggacctc ttctcgccga gaagcggtga tggagcgcaa gtttctccca tccgaggtcg
     1621 tcgagtagat ggcccccgcg ccgcgagatg ccatcacctc gtcggcatcg tctcggaaac
     1681 gctccggatc gaagaccagc cgactgacgt gattgacgaa tctcacgcct ccggcctcag
     1741 ccgccgacgc gaatagtcgg tcggtaaagt ggtcggttac gcgaaggact tcggcgcgta
     1801 gatcctcgtc accgagtaag agttccgagc ggatggtccg tgggatatgt gttcccgagt
     1861 gcgggatgtg tgcgatcaga gggagacgag ccctctcgac ggggtcgagg gtgaagggcg
     1921 cctgagtcat cggatcaccg atgcgacgaa ggccatctgg gcgagatggt aggtggcgat
     1981 gatggtcagg gactgccagg tgcgtgatcg aacgaatctg tcatgcgcga tcaggctgtc
     2041 ggaagccatg aatagaagag cccccatccc gagcaggatg tcgtgagtgc cgatcgcagt
     2101 ggccgccata gcgatgatgg cggcgatgta gatgcccact ggaagcatca ggggccggtc
     2161 accctgtctc aaagctctga acaccggtcg tgcaacccct actcccacca tgaccagcaa
     2221 ggggatcgcg atcagaaccg tccgcgggtg gtacgcgatc gatgcgaagc cgatgatgaa
     2281 agccaaatgc gccaggagga atgacccgag cccggcgatg aaaagatcgg cagggagcat
     2341 cagcgccaca tcaccgatca atgagagggc aagggccgcg atcacccagc tcttgcccgg
     2401 tccgtcgggg gccgcagttg cggaaagcag aaggaaaagc agagctgctg gtttgaacac
     2461 gtactccaga aacttgttcg tgcgtgcgac cgcgatccaa tcgatgagtg ctgccgctcc
     2521 accgagtgcg aatagggact gggctaggtt catgcgttct tggccacttc gcctcttccg
     2581 cccatcaccg acttgatgat
//
LOCUS       DATDOZ010000077         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3455688, whole genome shotgun sequence.
ACCESSION   DATDOZ010000077 DATDOZ010000000
VERSION     DATDOZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455688"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VI541_01485"
     CDS             <1..405
                     /locus_tag="VI541_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HLF69607.1"
                     /translation="ALLDSPRPLHGLLRDQRVIAGIGRSWVDEILHRTRLSPFARGEA
                     LTEEASLELRDSTIELLGGAIDHYADVLSIPIPDKMPMPLRIHRHEGEPCFRCGTRLE
                     AVHFEDHTMTYCPGCQTGGKLLKDRRLSRLIK"
     gene            complement(407..1270)
                     /locus_tag="VI541_01490"
     CDS             complement(407..1270)
                     /locus_tag="VI541_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF69608.1"
                     /translation="MESPIRFRPSPDLFPFESHWFESSVGPVHYIDVGEGRPIVFFHG
                     NPTWCFLYRKVIGALQSHYRCIAVDYPGFGLSVRPMDYGYSPAEHAKVMSEFVDHLGI
                     EDFMIMGQDWGGPIGLSVAASRPEAVTGLILGNTWFWPTDRLLNKIFSRVMSSSPMQR
                     RILERNFFIDRLIPMGSGSLLSDYELDQYRGAQPFGMRQGQAEFPRQLMSAGPWLATL
                     YEQVVEHLSRKRLLLVWGMKDIVFHPNAFLPKIRRVFHDSAMVQLPKAKHYIQEDAPG
                     EIAQAILQHFY"
     gene            complement(1279..1896)
                     /locus_tag="VI541_01495"
     CDS             complement(1279..1896)
                     /locus_tag="VI541_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLF69609.1"
                     /translation="MSEGTRVRMSGRERREQLLDIGKTLFAEKGFDAASIEEIAKRAD
                     VSKPVVYEHFGGKEGLYAVVVDREIERLLRSITDSLQATEPRMLLEQAAIALLTYIED
                     DQDGFKILTRDSPVASSSGTFASLIGDIASQVEHILVKEFRIRDYDTKLAPLYSHALV
                     GMVALTGQWWLEAGKPSRNKVAAHLVNLAWNGLARLETKPRISGA"
     gene            complement(1922..>2180)
                     /locus_tag="VI541_01500"
     CDS             complement(1922..>2180)
                     /locus_tag="VI541_01500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HLF69610.1"
                     /translation="NSICHFYGKRPFESGDLSTNNWLMALVAFGEGWHNNHHAFPSSA
                     RQGLEWWQIDPAGYLIALLAKLGLARDVKIPSSKQMAAKRV"
BASE COUNT          443 a          672 c          643 g          422 t
ORIGIN      
        1 gctctgctcg actctccccg gcccctccat ggactgcttc gcgaccagcg cgtgatcgcg
       61 ggcatcggcc gttcgtgggt cgacgagatc ctccaccgga ccagactctc tccttttgcc
      121 agaggcgagg cgctgaccga ggaggcatcc ctcgaactga gagactcgac catcgaacta
      181 ctcgggggcg cgatcgacca ctatgcggac gttctatcga tacccatccc cgacaagatg
      241 ccgatgcccc tacgcatcca ccgccatgag ggtgagccct gcttcaggtg tgggacgagg
      301 ctcgaggccg ttcacttcga ggaccacacc atgacctact gtcccggatg ccagactggc
      361 ggcaagctcc tgaaggaccg gcgactctcc cgactgatca agtgactcaa tagaagtgct
      421 ggaggatcgc ctgggcgatc tcgccaggag catcctcctg gatgtagtgc tttgcctttg
      481 gcagctggac catcgcagag tcgtggaaga cgcgccggat ctttggcagg aaggcgttcg
      541 ggtgaaagac gatgtccttc atgccccaga ccagaagcaa gcgcttgcgc gagagatgct
      601 cgacaacctg ctcgtaaaga gtggcgagcc acgggcccgc cgacatcagc tgacgcggaa
      661 actctgcctg gccctggcgc atcccaaagg gctgggcccc ccggtactga tcgagttcgt
      721 aatccgagag caatgagcca ctccccatcg ggatgagtct gtcgatgaag aagtttcgct
      781 cgaggatcct tcgctgcatc ggtgacgacg acatcaccct gctgaatatc ttgttcagga
      841 gcctgtcggt cggccagaac cacgtgttgc caaggatgag tccggtcacg gcctccggac
      901 gcgacgccgc tacggagagc ccgatggggc ccccccaatc ctgacccatg atcatgaagt
      961 cttcgatccc aagatggtcg acgaactcgg acatgacctt ggcgtgctcg gcaggcgaat
     1021 acccgtaatc catcgggcgt accgagagtc cgaagcctgg atagtcaacc gctatgcatc
     1081 ggtagtgcga ctgaagtgcg ccgatgacct tgcgatagag gaagcaccac gtcggattcc
     1141 cgtggaagaa gacgatcggc cttccctcac cgacatcgat gtagtggacc ggccccaccg
     1201 aactctcgaa ccaatgagac tcgaatggga agagatcagg tgatggacgg aagcggatgg
     1261 ggctctccat ctcggtgctc aggcccccga gatccggggt ttcgtttcaa ggcgcgccag
     1321 accattccat gcgaggttca ccagatgtgc cgccacctta ttccggctcg gcttgcccgc
     1381 ctcgagccac cactgtcctg tgagtgcaac catcccgacc aacgcatgcg aatagagcgg
     1441 agcaagcttc gtgtcgtagt cacggatgcg aaactccttg acgaggatgt gttcgacctg
     1501 cgaagcgatg tctccgatca ggctggcgaa ggtaccggaa gaactcgcga ccggcgagtc
     1561 tctggtaagt atcttgaagc catcctggtc gtcctcgata taggtcagca gggctatggc
     1621 cgcctgctcc aaaagcatcc gcggctcggt agcctgcaag gagtcagtga tcgaacggag
     1681 gagtctctcg atctcgcgat cgacgacgac agcgtagagc ccctccttcc cgccgaagtg
     1741 ttcatatacg accggcttag agacatcggc tcgcttcgct atctcttcga tcgaagcagc
     1801 gtcgaagcct ttctccgcga agagagtctt cccgatgtcg agtaactgct cgcgtcgctc
     1861 tcgcccggac atccggacgc gcgtgccctc actcatgtcg ctgatcgcca tgctggatga
     1921 ttcaaacgcg cttggctgcc atctgcttgc tagagggtat cttcacgtcg cgggccaagc
     1981 cgagcttagc taggagggcg atcaggtacc cggccgggtc gatctgccac cactcgagcc
     2041 cctgacgggc agagcttggg aatgcgtgat ggttgttgtg ccaaccctcg ccgaaggcaa
     2101 ccaacgccat cagccagttg ttggtgctga gatccccgct ttcgaatggg cgcttgccgt
     2161 agaagtggca gatcgagttg
//
LOCUS       DATDOZ010000078         4286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3456648, whole genome shotgun sequence.
ACCESSION   DATDOZ010000078 DATDOZ010000000
VERSION     DATDOZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3456648"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VI541_01505"
     CDS             complement(<1..714)
                     /locus_tag="VI541_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69611.1"
                     /translation="MAPLTAGHADASLAPLLSRASFTTGFDGWAAVGEAQTSRILDAA
                     DPVLEIHAGSALSAALPEGVSKLQLSYRDLDPYCCSATKLVELRDSSGSPLLTLEQYS
                     STLVVTGASVQYVGHHADTGWHRLTLDLSLESIAIRFDDILWASAAPASSNSLGRVAA
                     ATGQGRRLLLDDVMAFGPGAVRSDSFYYTIPDGEFQFVDSGPYGRTERQSGASAARDV
                     GYARVIGGPSPGEVHNVTWP"
     gene            complement(942..2600)
                     /locus_tag="VI541_01510"
     CDS             complement(942..2600)
                     /locus_tag="VI541_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69612.1"
                     /translation="MKPRSLFRTLLYSHLIVGSIALAILGIALDRVLERKALDSLVSR
                     LTSEARSVQTIHDEIPAADLQTRVASLGRASSSRITIVRHDGSVLADSEHDPATMANH
                     ATRPEIRQALQGREGVSQRESPTLGVPFLYVATPERDGIVVRAAFPATALRVQREAIR
                     NTIAISLLLVGVLTVLLSALLSRSLGRPLHRMSREVAEVAAGSRKVVQATGSAEARGL
                     AEAVNRMAEDLARRADELSKESSLRDQILSSMSEGVILGDPSGKLVYANAAASRLFGR
                     EHLSEIPFQVTEPGFHEVAIHHPTRRDLRSETVTLDDGRLLVAVQDVTEAKRLDDVRR
                     DFVGNASHELKTPTASILATAETIATAAGDDPDAVSGFSERMVQEATRLSRIVEDLLD
                     LSRLEQGPRERVEVSLDGIVSSEVDQILGAAKHRGLEVELVSSPELRVQGSEEDLRLM
                     VRNLLDNSVRYTNQGSITVTLNSNAGRAILTVEDSGAGIPAEDLPRIFERFYRVDRAR
                     SRETGGTGLGLSIVRHVAESFGGTVRVESQLGSGSKFTVELPQS"
     gene            complement(2597..3295)
                     /locus_tag="VI541_01515"
     CDS             complement(2597..3295)
                     /locus_tag="VI541_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF69613.1"
                     /translation="MPNVLVVEDDPTMSEMVAYNLRRQGLTVEVASDGTSGLKLARKN
                     STALVILDLMLPGIDGLQIAQELRKARPWLPILMLTARSEESMKLKGFSAGVDDFLTK
                     PFLMDELMARVKALLRRSRFEALRNDVVDELIFGDLRIQPADFRCWIADKEIQLRPKE
                     LALLATLAGRPGHLFGRLELAEQVWGYAHLGDTRTIDTHVKNLRRKVETTSQFNYIET
                     VRGLGYRFKVSLKR"
     gene            3446..>4286
                     /gene="pstS"
                     /locus_tag="VI541_01520"
     CDS             3446..>4286
                     /gene="pstS"
                     /locus_tag="VI541_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HLF69614.1"
                     /translation="MLKISAPVLALILLAVGCGGSGTNVIDPKGSPAAKDLQGVSLTG
                     AGATFPAPLYQLWASEFQKESGLQVNYQSIGSGGGIQQITARTVDFGASDAPMKDEEL
                     AKADAPLLHIPMALGAVVITFNLPEIAKEIRMNSDTLAAIFLGKIKKWNDPAIAADNA
                     GVSLPRSDITVVHRSDGSGTTSIFTSYLRDVNAEWSQKVGAGKEVTWPVGLGGKGNEG
                     VTALVKQTPGSIGYVELVYAEQNKLPPVSLKNSSGRFIAPSLASITAAAKGISVPDDL
                     RFSI"
BASE COUNT          886 a         1291 c         1246 g          863 t
ORIGIN      
        1 gggccaggtg acgttgtgga cttctccggg cgatggcccc ccgatgactc tggcgtatcc
       61 gacatcgcgc gcagcactcg cccccgactg tcgctccgtt cttccatatg gacccgagtc
      121 cacgaactga aactcaccgt cagggatcgt gtagtagaag ctgtcactcc tcacggcgcc
      181 ggggccaaag gccatgacgt cgtccagaag aagacgtcgg ccctgccccg tggcagctgc
      241 gacgcgacca agtgagttgc tcgatgccgg ggccgcgctt gcccagagaa tgtcatcgaa
      301 gcggatagcg atcgattcca gcgagagatc tagcgtcagc ctgtgccacc cggtgtctgc
      361 atgatgtcca acgtactgaa cggaggcccc ggtcaccacc agggtcgagg agtactgctc
      421 caaggtcaga aggggcgacc ctgacgagtc gcgcagctcc acgagctttg tcgccgagca
      481 gcagtacgga tctaggtcgc ggtacgagag ctgcagcttg gagacgcctt ccgggagggc
      541 cgccgataac gccgaaccgg catggatctc cagcacaggg tcggccgcgt cgagaatccg
      601 gctggtctgc gcctctccga ccgcagccca gccatcgaac cctgttgtga aggatgcccg
      661 cgataggagc ggcgccagag aagcgtccgc gtgcccggct gtcagcggcg ccaaggccgc
      721 cactatgagg caaacgagcg cggccgtctg ccatgttttc gtaacggatc tcgcccgagg
      781 tcgcattccg gtcacggtgg ctcaattcgc cgcgagccgg gcaaaatcct cctgggtggg
      841 acaaatcacg cggtgtgaaa cgggacgaga tcgccggcga tcaggagcgc ttcccatgat
      901 tgtctggttt gatcgccctc tgtgtgcttt ttgtgtacta gtcagctttg cggcagctcc
      961 acggtgaact tgctaccaga tcccaactga ctttcaactc ggacggttcc cccaaagctt
     1021 tcggccacgt ggcgcacgat ggacaagccc aaccccgtgc caccggtttc tctggatcgg
     1081 gctctgtcaa cgcggtagaa acgttcgaag atccgaggaa gatcttcagc gggtatcccg
     1141 gcccccgaat cctcaaccgt caagatcgcc cttccggcgt tcgagttcag ggtcacagtg
     1201 atggagccct ggttcgtata acgaacggag ttgtccaaga gatttctcac catcaaacgc
     1261 agatcttcct ccgatccttg gaccctcaac tctggcgaag agacaagttc aacctcgagg
     1321 cccctatgtt tggcggcacc aagtatctga tcgacctcag atgagacgat gccatcaaga
     1381 ctcacttcaa ccctttctct tggcccttgc tcgaggcgag aaaggtctag gaggtcttcg
     1441 acgatgcgcg agagtcgcgt ggcctcttga accattctct cgctgaatcc gcttacagca
     1501 tcagggtcgt ctcccgcagc agttgcgatc gtctcggcgg tcgctaggat cgacgcagtt
     1561 ggagtcttga gctcgtgaga tgcatttcca acgaagtctc ttcgcacatc gtctaatcgt
     1621 ttagcctcgg tcacgtcctg aaccgctacc aacaaccggc catcgtcaag tgtcacggtt
     1681 tccgatcgaa ggtctcttct tgttgggtga tggatcgcga cttcgtggaa accgggctcc
     1741 gtgacttgga atgggatctc cgacagatgt tcgcgcccga acagccgcga ggcagctgcg
     1801 ttcgcgtaaa ccaacttccc ggacgggtcc ccaaggatca cgccctcact catcgaagac
     1861 aggatctgat cacgcaggga tgactccttg ctgagttcat ctgcgcggcg ggccaagtcc
     1921 tctgccatgc ggttgacggc ttcggccaga ccgcgagctt cggcggaacc tgtggcttgc
     1981 acaactttgc ggcttccggc cgcaacctca gcgacttctc gactcatgcg atgcagggga
     2041 cgaccgagcg atcgagaaag tagcgcggat agaagtactg tgaggacgcc aaccaacagt
     2101 aacgaaatgg ctatcgtgtt tctgatcgcc tcgcgctgga ctcgcagcgc agtcgctggg
     2161 aaggcagctc tcaccacgat cccatcgcgc tcaggtgttg cgacatacaa gaagggcaca
     2221 ccgagcgtcg ggctctcgcg ctgcgatact ccctctcgcc cttggagcgc ctgacggatc
     2281 tcgggtctgg tggcgtggtt cgccatggtc gctggatcat gctcggagtc cgcgagaaca
     2341 gagccgtcgt gacgaactat cgtgatcctg gaagacgacg ccctgccgag agacgcgact
     2401 cgcgtctgga gatctgcagc aggtatctcg tcatggatcg tttgcaccga acgcgcttca
     2461 gaggtcaagc ggcttaccag cgagtcgagg gcctttcgtt caaggacccg gtcgagtgcg
     2521 atccctagga tcgctagcgc gatcgaacca actatcaggt gactgtaaag aagcgtacgg
     2581 aagaggctgc gaggcttcat ctcttgagtg agaccttgaa tcgataacca agccctctaa
     2641 ctgtttcgat gtaattgaac tgagacgttg tttcaacctt ccggcgaagg ttcttcacat
     2701 gggtgtcgat ggtgcgcgtg tcgcccaggt gtgcgtaacc ccacacctgc tctgccagtt
     2761 ccaacctgcc gaagagatga ccgggcctgc cagcgagagt tgcaagtagc gccagctcct
     2821 tggggcggag ctgaatctcc ttgtctgcga tccagcatct gaagtcggcg ggttggatcc
     2881 tgaggtcacc gaagatgagc tcatccacga cgtcgttgcg caaggcctcg aacctcgagc
     2941 gacgcagcag tgccttcact cgcgccatca attcatccat caagaatggt tttgtgagga
     3001 aatcgtcgac gccggcggaa aagcctttca atttcatcga ttcctccgac ctggccgtca
     3061 acatcaagat tgggagccag ggccttgctt tgcgcagttc ttgggcgatc tggagtccat
     3121 cgattccagg caacatcagg tcgaggatga caagcgccgt tgagttcttg cgagcaagtt
     3181 tgagaccgga ggtcccgtca cttgcaactt caacggtgag cccttgacgc ctgaggttgt
     3241 aggcgaccat ctcggacatc gttggatcat cttcgacaac tagaacgttg ggcaacatgc
     3301 ctcctgccgc tcatccgtcc cgatgagcga tccgatagta accgttccgg tgtcatttaa
     3361 gttcacagag agttcgcgag gagcacaccc aagccatcgg ggcaacacct acgatcgtcc
     3421 cgtcctacat caaggagggg catcagtgct aaagatctcg gcgccagtgt tggcgctgat
     3481 cctgttggcg gttggctgcg gtggttccgg caccaatgtc atcgatccga aggggtcgcc
     3541 agcagccaaa gacctgcagg gtgtaagcct taccggggca ggggcgacat ttcctgcacc
     3601 gctctatcaa ctgtgggcat ccgagttcca aaaggagtcg ggcctgcagg tcaactacca
     3661 gtcgatcggc tccggcggag gcatccagca gataaccgca agaactgtcg acttcggcgc
     3721 tagcgacgca cccatgaagg atgaggagct cgccaaggcc gacgctcccc tcctgcacat
     3781 cccgatggct ctgggagcag ttgtcatcac cttcaatctc cccgagatcg ccaaagagat
     3841 aaggatgaac tcggacaccc ttgccgcgat cttcctcggc aagatcaaga agtggaacga
     3901 cccagccatc gcggctgaca atgccggggt cagtctcccc cgttccgaca tcacagtcgt
     3961 acaccgttcc gacggttcag gcacgaccag catcttcacg agttacctga gagatgtgaa
     4021 cgccgaatgg tcgcagaagg tgggggccgg aaaggaagtt acctggccgg taggcctcgg
     4081 cggcaagggc aacgagggag tgaccgcact cgtcaagcag acgcctggat cgatcggcta
     4141 cgttgaactc gtctacgccg agcagaacaa actgccacct gtctctttga agaactcgtc
     4201 gggcaggttc atcgcgccaa gccttgcttc gatcaccgcg gcggcgaagg gaatctcggt
     4261 ccccgacgac cttcggttct cgatct
//
LOCUS       DATDOZ010000079         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1374571, whole genome shotgun sequence.
ACCESSION   DATDOZ010000079 DATDOZ010000000
VERSION     DATDOZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374571"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VI541_01525"
     CDS             complement(<1..834)
                     /locus_tag="VI541_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HLF69615.1"
                     /translation="MSAQQEQQAVTMGRLLDQRARELLLTLFEHRLLSTSQLKVLYFS
                     SLRRCQDALKKLCDLALIARDRPFQQIGTGKPESLWTLTEAGVRIVAVTMRKARSQLD
                     WMPRDSFHVSDRQLDHLLGVNRFFVSLVEASLAHPGHGLEKWVVAGQVKSQNNWVTHD
                     GFGRYQHQGGACDFYFEYDRGTEWHDQLVRKLRGYVLMAIKWTQEGPAHFPNVLVLVP
                     DEKREKAFDRAMKAAVESLEIRAKVAVALPMFITSEDRLTGDGMLGKIWRTFAPAPAD
                     RP"
     gene            complement(872..1039)
                     /locus_tag="VI541_01530"
     CDS             complement(872..1039)
                     /locus_tag="VI541_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69616.1"
                     /translation="MGSESSSSLEKPRFVAERLHDRVHPPQQRGDKVRLRFALLNQGL
                     ESATKHTIGMR"
     gene            complement(2164..>2421)
                     /locus_tag="VI541_01535"
     CDS             complement(2164..>2421)
                     /locus_tag="VI541_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69617.1"
                     /translation="GDTKPTLDVFVRDRVAGKTHLVSALPDGSQGYLDAVAPSISADG
                     NYLAFDTQLAPYQIMNNYFRSAYLRQLWTPCSIVCLPNPAL"
BASE COUNT          464 a          727 c          721 g          509 t
ORIGIN      
        1 cgggcgatca gccggagcag gagcgaacgt cctccatatc ttccccagca ttccgtcccc
       61 cgtgaggcga tcttctgacg tgatgaacat cggaagcgcg actgcgactt tcgcacggat
      121 ctctagcgac tcgaccgctg ccttcatcgc ccggtcgaat gctttctcgc gcttctcgtc
      181 gggcaccagg acaagaacgt tggggaagtg agccggcccc tcctgggtcc acttgatcgc
      241 catcaggacg tatccccgaa gctttcgaac caactggtcg tgccactcgg tgcctcggtc
      301 gtactcgaag tagaagtcgc atgcaccgcc ttgatgttga tatcgaccga acccatcgtg
      361 ggtgacccag ttgttctgac tcttgacctg cccggcaacc acccacttct ccaggccatg
      421 gccgggatga gcaagcgacg cctcgacgag ggagacgaag aacctgttca ccccgagcag
      481 gtgatcgagc tggcggtcgg aaacgtggaa gctgtctctg ggcatccagt cgagctgaga
      541 gcgagcctta cgcatagtga ccgccactat ccggaccccg gcttcggtaa gtgtccatag
      601 agattcgggc ttacccgtac cgatctgctg aaagggccga tcccgcgcga tcaacgctag
      661 gtcgcagagc ttcttgagag cgtcctgaca tctgcgaagc gatgagaagt aaaggacctt
      721 gagctgagac gtcgagagca gccgatgctc gaagagagtg agcaggagct cgcgagcacg
      781 ctgatcgaga aggcgaccca tcgtgactgc ctgctgctcc tgctgtgcgc tcatgtcggc
      841 gttcctttct cctctccctc ttgcatcacc ctcacctcat cccgatggtg tgtttggtcg
      901 cactctcgag cccttgattg agaagcgcga accgaagacg aaccttgtcg ccgcgttgtt
      961 ggggagggtg cacccggtcg tgaagtcgct ctgcgacgaa gcgaggtttc tcaagactcg
     1021 acgacgattc agaacccatt gggcgcggcc ctcccccctc cttcgccatc ggaggccttg
     1081 ggcaaccggc acatgacctg gggttttgcc aagtcatcgc cttagcccag cgccacgaaa
     1141 acgccattcg tctcctcggc ttttccgaag acggttgaaa gtcgctgcgg tagcaagtgc
     1201 agttcaaaca tttgcaactc caatgtcgtg ccgttgtaca agcagcaagt tctttccgat
     1261 tcgaagcctt ggccgacgcg atcgtcgtgc tctgcgaaag cctttcggca acgcctgact
     1321 gcgtcccttc caatcgcaca cgctcgtctc catgtgcaac gccactcacc gagcgcgcct
     1381 cgccattcga ggtgctcggg gtgcgtgcca ttgaatccga tcggctcgga tgggtgcagc
     1441 ccttcgtgct tccaagccat cgcttggctc gactcgctcg gcagtccttc ggcaggatga
     1501 cgtcagatgt ccaaacgcct ggtcctcgaa actgaggacc tgacgcaagg cgcatagggt
     1561 cgccctgaga aggaagtgcc gactgacgag agcgatccgt cgctgggctt gtttcgttga
     1621 actcttctcc tttctgaatt gtcgggagag cagctgggtt tgcggctctt actattaaga
     1681 gcaagttcgg gcccgattct gtgacacgtt ttcaggcgcg aaccgtggga ccttgcgaca
     1741 tttccgcgcg acctgagcgg gcgggctaga caagtcgctc gcgcctatgg tcaagatgtg
     1801 aaggccccgt aggtgattga tagctcggcg tctgctgatc ttgaggccag gatcagggtg
     1861 accggctagg cagtcgtagc gcggtagatg tcgctattga ctcagtatct tcggagcgca
     1921 gagggtccca ggtctcgagg gcgacgtcgc gtccgtccgc ggaaaccagg cgtagccacc
     1981 ctcaccttgt gcgacgttcc cgtagtgtga gatggtgtgc tcgcccaccc acaggatcgg
     2041 cccctccagg aggggcaatc agtccacaag cagggcttac cgcgagcgcg tcaattgcgt
     2101 atctcactca tcctcattcg acactctccc tcagaatcct tgcgcgggac tgatcgatgt
     2161 ggatcagagc gcggggttgg gaaggcacac gatagagcag ggggtccaaa gctgtctcag
     2221 gtacgcggac cggaagtagt tgttcatgat ctggtagggg gccaactggg tgtcgaatgc
     2281 cagatagttt ccatcggctg atatcgatgg agcaaccgcg tcgaggtagc cctgactgcc
     2341 atcggggagg gccgagacga ggtgggtctt tccggccaca cggtccctca cgaagacatc
     2401 gagtgtcggc ttggtgtccc c
//
LOCUS       DATDOZ010000080         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1886482, whole genome shotgun sequence.
ACCESSION   DATDOZ010000080 DATDOZ010000000
VERSION     DATDOZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886482"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VI541_01540"
     CDS             complement(<1..532)
                     /locus_tag="VI541_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLF69618.1"
                     /translation="MPKILIGTASWTDKSLLQSGFYPKGADSAEERLRFYSERFPIVE
                     VDSTYYFPPTDRNAALWAERTPGHFTFHIKAYSLFTQHPTRTASLFGDIREALRPQVA
                     DRPRLYAKDLPQEAIDQMWTRFADALMPLHSSGKLGLVHFQFPQWFTPNSKNRDYILE
                     CKQRLHDYRMGVEFRNG"
     gene            590..>2177
                     /locus_tag="VI541_01545"
     CDS             590..>2177
                     /locus_tag="VI541_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLF69619.1"
                     /translation="MALKVLLLGLDDVTARRVQNAFLSEAVSFVTGERLADVDIASSL
                     DLVVANTNIEDPDALTEKCSSASVPLLTMDAKDPRRATVVAADLPALLARLYAGVGTP
                     ALARLAEAQQKAHLGNWEWDVIGDYVVWSDELYRLFGLEPGSIEIGYDTYLRLIHPDD
                     QSRADSVVRTAMETHEDFAFDHRVILPNGETRVIHGTGSVQLDGSGRVIRMAGTAQDI
                     TERVMIQETVERLAAIVESASDCIVSTNLDGVITSWNRGAEAMYGYSAADVVGRNVEL
                     LIPDDRHGEGRRLLKVSNKGVDIPEYQTTRKRQDGSLVDISLTISPVRDRWGAVVGAS
                     RIARDITAQKRAEQNVREVHEAQERLIENMRNLEELRMNFLSTVSHELRSPLTPVIGY
                     ANTLKRRWKTLTEKEREDFIDRIVRNSERLDTLIGELLDFSRLESGKLGVSITKLHLG
                     EQFSAVISNMPELLASHEVELGLTDGVAVQADPRLLARIIENLLTNAAKFSPPGSRIV
                     IDAEKSDGAVVVSVADEGPGI"
BASE COUNT          451 a          595 c          706 g          425 t
ORIGIN      
        1 gtccgttgcg aaactccacc cccatccgat agtcgtgcag ccgttgcttg cactcgagga
       61 tgtagtcgcg gttcttggag tttggggtga accactgcgg gaactggaag tgcacgaggc
      121 cgagtttgcc cgaggagtga aggggcatca gcgcatcggc gaagcgtgtc cacatctggt
      181 cgatggcctc ctgggggagg tccttggcgt agagccgagg gcggtcggcg acctgggggc
      241 gcagggcctc gcgtatgtcg ccaaagagcg aggccgtgcg agttgggtgt tgggtgaaga
      301 gcgagtaggc cttgatgtga aaggtgaagt gcccgggagt gcgctcggcc cagagcgcgg
      361 cgttccggtc ggtgggtggg aagtagtagg tggagtcaac ctcaacgatc gggaatcgct
      421 cggagtagaa gcgcaggcgc tcctctgcgg agtccgcacc tttggggtag aacccgcttt
      481 gcagtaggct cttatcggtc caggaggccg tccctatgag gatcttgggc atggctgagt
      541 ctagacacga gcagtggcgg atgtagctga acggaggcga tactaggcca tggctctgaa
      601 ggttcttctg ctcggtcttg atgacgttac ggcgcgaagg gtccagaacg catttctcag
      661 cgaagccgtc tccttcgtaa caggcgagcg tcttgccgat gtcgatatag cctcgagcct
      721 cgacctggtg gtagccaaca ccaacatcga agaccctgat gccctgaccg agaagtgctc
      781 ctccgccagc gttccgcttc tgacgatgga tgcgaaggat ccgagaaggg cgaccgttgt
      841 tgccgccgac ttaccggctc tactcgctcg cctttacgct ggtgtaggca ctccagctct
      901 ggcaaggctg gcagaggccc aacagaaggc ccacctcggc aactgggagt gggatgtcat
      961 cggcgactat gtcgtctggt ctgatgaact ctaccgactc ttcggcctcg aaccaggttc
     1021 gatcgagatc ggttacgaca cctacctcag actcatccac cccgacgacc aatccagggc
     1081 cgactctgtc gttaggaccg cgatggagac tcatgaggac ttcgcctttg atcacagggt
     1141 gatacttccc aacggcgaga cccgagtcat ccacggaaca ggtagcgtgc agctcgatgg
     1201 ttccgggcga gtgatacgga tggcggggac agctcaggac atcaccgaga gggtgatgat
     1261 ccaggagacc gtggagcgat tggcagccat cgtcgagtct gcgagcgact gcatcgtgag
     1321 cacgaatctt gatggagtga tcacctcttg gaataggggg gccgaagcga tgtacggcta
     1381 ctccgctgcc gatgtcgtcg gacgtaatgt cgaactgctc atcccggatg accgccacgg
     1441 cgaaggacgt cgactcctca aggtttcgaa caagggggtt gatatccccg aatatcagac
     1501 gacgcgcaag cgccaagatg gcagcctcgt tgacatctcc ctgacgatct ccccggtgag
     1561 agacaggtgg ggcgcggtgg tgggggcctc gcgaatcgcc agggatatca ctgcgcaaaa
     1621 acgggctgag cagaacgtgc gtgaggttca cgaggcgcag gaacgactga tcgagaacat
     1681 gagaaacctc gaagagcttc ggatgaactt tctgtctacg gtttcgcatg agttgaggag
     1741 ccccctaacg cctgtgatcg ggtacgccaa tacgctcaaa cgtcggtgga agacccttac
     1801 cgagaaggaa agggaggact tcatcgaccg gatcgttcgc aactccgaga ggctcgacac
     1861 cctcataggt gaactgctcg acttctcgag gcttgagagc ggcaaactgg gtgtgtcgat
     1921 caccaagcta catctcggcg agcagtttag tgccgtgatc tcgaacatgc ctgaactgct
     1981 cgccagccac gaggtcgagc taggtctgac agatggtgtg gccgtccagg ccgacccgcg
     2041 cctgctcgcg cgcatcatcg agaacctatt gacgaatgca gccaagttct ctccgcccgg
     2101 ttcgaggatc gtcatcgatg cagagaagtc ggatggggcc gttgtcgtta gcgtcgccga
     2161 tgaaggtccc gggatcc
//
LOCUS       DATDOZ010000081         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2934059, whole genome shotgun sequence.
ACCESSION   DATDOZ010000081 DATDOZ010000000
VERSION     DATDOZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2934059"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="VI541_01550"
     CDS             <1..2052
                     /locus_tag="VI541_01550"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF69620.1"
                     /translation="SAGRFAEAVPVSMAEADAAAATFAFSEAVDLWERALPHIDDSLV
                     KARVFCRIGEALGMNSESDRAARNLTIAVDQLEAAGGLPTEAAHYRLLLGRALWENGD
                     SDGAMSEYEAAREVLEREPPSADLALAYMRIASHHAFQLDYAVCGEWAQRAVNIAEAT
                     GAAKERLWATGFLALSLVVEGRVQDALALFAESIAECIESGNPVVGVNLIYNETWLRA
                     HLTIPMRGSVQRLDLLPQVPATVVARAMLACDEKIAGGDLAAAVESAERGLELATANG
                     FGKMIWRSSVSLAEALYEQGRYADSQAVLPKISTRSETQDVFYDGVVRMRLAIANGET
                     DHALDVARSLAALGDKVSVYRRDLAAAAETLTACGELEEAEALMSVCRGNLANAGLAR
                     LDAASARLRLAQGDPQDASRYCESALGELSDSGYVLEDLRARITLAEVQATLGENSLA
                     EMTLGSLLDRANDLGAGAVIDDAIKMAERVGLSMDRPVVADPSDESTESAIPLGERMI
                     TSMFADIRSYTPMTVSTPPHDLADKMATLFRWAKDEVERHGGVVDKFAGDAVMATFNV
                     SGASVDHPVQALSAALALREKVRLASIPMGIGIAVGPAIVGNAVTGGHLMVAGVATNL
                     AARLQSAAKAGEIILSDEAYRRLEGQVEAMGLKVHREQLELKGFDEAQTVFRLSNGPA
                     G"
BASE COUNT          413 a          597 c          697 g          414 t
ORIGIN      
        1 agcgctggcc gtttcgctga ggcagtacca gtctcgatgg cggaagctga tgccgctgca
       61 gcgacgttcg ctttctctga ggccgtggat ctgtgggagc gggctctgcc ccacatcgat
      121 gactcactcg tgaaggcccg cgtcttctgc aggatcggcg aggctcttgg gatgaatagt
      181 gagtccgatc gagccgctcg gaacctcacg attgctgtcg accagctcga ggccgcaggc
      241 ggactaccga cagaggcggc tcactaccgt cttctgctcg gaagggcgct atgggagaac
      301 ggagattccg atggcgcgat gagcgaatac gaggcagctc gtgaggtgct cgaaagggaa
      361 cccccatcgg ctgatctggc gctggcgtac atgaggatag cttcccatca tgcgtttcaa
      421 ctcgactacg cagtttgtgg agagtgggcg cagcgtgccg tcaacatcgc ggaggcgacg
      481 ggtgcggcga aggagcgcct ctgggcgaca ggattccttg cgctgagtct cgtggtcgag
      541 gggcgcgtcc aagatgcttt agccttgttt gccgagtcca tagccgagtg catcgaatca
      601 gggaaccccg tcgttggtgt caacctgatc tacaacgaaa cgtggctgag ggctcacttg
      661 acgatcccga tgcgggggag cgtgcagaga cttgatctgc ttccccaagt gccagcaacg
      721 gttgttgcgc gagcgatgct tgcgtgcgac gagaagatcg caggtgggga ccttgcggcc
      781 gccgttgaat cggccgaacg cggattggag ttagcaaccg cgaatggctt cggcaagatg
      841 atctggcgct catccgtttc cctcgccgag gcgttgtatg agcagggccg gtatgcggac
      901 tcccaggccg tgttgcccaa gatctcaacg cgctcagaga cccaggacgt tttctacgac
      961 ggagtcgtga ggatgaggct ggcgattgcc aacggcgaaa ccgatcacgc cctcgatgtc
     1021 gcgcgatcgc tagcggcctt gggcgacaag gtgagcgtct atagacgaga tcttgccgca
     1081 gccgctgaga ctctcaccgc gtgtggcgaa ctcgaggaag ccgaagctct catgtcagtc
     1141 tgtcgaggca atctcgccaa cgccggcctt gctcggctcg atgcagcctc ggctcgactc
     1201 agactggccc aaggcgaccc gcaagatgcg tctagatact gcgagagtgc cctgggggag
     1261 ctgtctgata gcggctatgt gctcgaggac cttagagcaa ggatcaccct tgctgaagtg
     1321 caggcgacgc tcggggaaaa ctctttggct gagatgaccc ttggcagcct gctggataga
     1381 gcgaacgatc tcggcgcggg ggccgtgatc gacgacgcga tcaagatggc agagcgagtc
     1441 gggctgtcca tggaccgacc tgtggttgct gacccttcag acgagtcaac cgaaagtgcc
     1501 atccccctcg gagagcggat gatcacttcg atgttcgcag acatccgcag ctatacaccg
     1561 atgacagtgt cgacaccccc gcacgatctg gcggacaaga tggccacact cttccgttgg
     1621 gcaaaggatg aggtcgagag acacggcgga gtggtggaca agtttgcggg cgacgcggtc
     1681 atggccacct tcaatgtttc gggtgcatcc gtggatcatc cggtgcaggc attgagtgcg
     1741 gctcttgcct tgcgagaaaa ggtgcgtctc gcatccatcc cgatgggtat cggtatcgcc
     1801 gtagggcctg ccatcgtcgg gaacgcagta acaggagggc atctgatggt ggcaggggtt
     1861 gctaccaatc tcgctgctcg cctccaatcc gcggccaagg ctggcgagat catcctgagc
     1921 gatgaggcat accgccgact cgagggtcag gttgaagcga tgggactcaa ggtgcaccgt
     1981 gagcaactag agctgaaggg tttcgatgag gctcaaacgg ttttccggct gagcaacgga
     2041 ccggcgggct aggccccctc gatcactgcg agttgggccc ccgcagagac cgtgtccccg
     2101 gctttgactc tcatctcctt c
//
LOCUS       DATDOZ010000082         4533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_66464, whole genome shotgun sequence.
ACCESSION   DATDOZ010000082 DATDOZ010000000
VERSION     DATDOZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66464"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VI541_01555"
     CDS             <1..618
                     /locus_tag="VI541_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLF69621.1"
                     /translation="VALLARSGAEIGEVSLPTFESGIDIYYLIAPAEASANLARYDGV
                     RYGLRVEGKDIFQMNALTRSAGFGPEVKRRIMLGTYALSAGYYDAYYGKAQKARTLII
                     RDFERAYERYDLLVSPASPTTAFRFGEKTSDPLTMYLSDICNVPTSLAGAAAISVPCG
                     TAPEDGLPVGLQFMSRPLDEVRLLKVAYAFEQSLGFDSKPPMWGA"
     gene            618..2063
                     /gene="gatB"
                     /locus_tag="VI541_01560"
     CDS             618..2063
                     /gene="gatB"
                     /locus_tag="VI541_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLF69622.1"
                     /translation="MAHNHVTTIGLEIHVELHTATKMFCACPVGFGGEPNSRVCPVCL
                     GHPGTLPVPNERAIEFTEKIALALGCEVARRSIFHRKNYFYPDMPKNYQISQYDIPVG
                     SRGVVEVEVEGVTHRVGITRVHLEEDTGKSLHMGGEGRIHGADYSLEDFNRAGTPLVE
                     IVTEPDITSAEVAKAFAQELRTILEFLEVSDVKMEEGSLRVDANVSVAPPGERGVKLE
                     VKNMNSMRSLYRALLFEEARLQTALETGEEIVQATRHWDEKAGVTRIGRTKEFSEDYR
                     YFSEPDLCPIEPSVEWLRQLREELPESPSDRRKRFTDEGLSAYDAGVLTSSKVIADFF
                     ERASGVSSAGAKPVANWIANDLIGLLSERSEDLSQASITPEALAALVDKVAEGAISKN
                     QGKTALAEMLATGGSLDEVIEKGGLAQVSDRAELEAAVDEALRANPEIVERLKAGDRK
                     PMGFLIGQIIKATKGKGNPAIINEILKERIG"
     gene            2060..2638
                     /locus_tag="VI541_01565"
     CDS             2060..2638
                     /locus_tag="VI541_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF69623.1"
                     /translation="MNTSDPLLDWIRDEYRWVRSMIERDVLKGLSSEEIKTQLPTGNS
                     IAWVMWHLARIEDMAVTSVIRREPQVFLSFGSRLGTKQTGTGNSFGDEELAELTDSLD
                     VVVLLEYWREIGVGTRDWLGSVTLEELTKPISFEELAKVFPEGVVGIPSYSVRDWDGK
                     PPAYLLKWPVILHGYIHIGEMVTIRGSLPKTD"
     gene            2651..>4533
                     /locus_tag="VI541_01570"
     CDS             2651..>4533
                     /locus_tag="VI541_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLF69624.1"
                     /translation="MGRLSKLMVVEDDDTHFERLRSVLSSQPVSLIRVHSGDDRWRHL
                     DGAVDVLLLTVSSLTDVYEIDQVSGSIPIVVMTGQQDQELALIGLARGAVGHLFRESI
                     DADSLMRTLRYSIERFRVQRSLLETQANYKVLFANIPVPKWVYDEMTLRFLAVNDAAV
                     ETYGYSEAEFLSMSVRDLLIEEDLRRGLEELGRTIDRGALPPEGVVAENAVRHRVRSG
                     AIIDIEVASSHLSFEGRPAIFCAISDVTARRRAEARLAAQHAVTRVFAEAEDLSRAGP
                     QILQAIGESLGWDLGVLWLMDRVTRGLHCISLWKGHRVFAPDSERLTRSARLSPREGI
                     PGTVWAGRSLRWFGDLKDDPTERIAAAHREGFRSALAVPVGEGIPRGVLEFLTRESRA
                     PEPDVIALVETVGTQLGLFLERKEATADLREREVRLSAILRSSLDSIILMDHRGLITE
                     FNDSAERVFGFTRDEAVGRLLADLIIPPDQRKSHSEGLRRFHETGEEHVIGKRIERTA
                     LRADGSTFPIELTVTAMDLPGPAMFVGSIRDLTTQKQATAALRVSEGKYRQLLEQASD
                     GIAVADAQGRLETVNPRMCQLLAQEEKDLVGLRFVDLIDLEEGGAGALDAVVPGESVL
                     VDGS"
BASE COUNT          938 a         1279 c         1431 g          885 t
ORIGIN      
        1 gtggcgctgc tagcgcgttc gggggccgag atcggcgagg tgtcccttcc gacattcgag
       61 tcggggatag acatctacta tctgatcgca cccgcagagg cctctgcgaa tcttgcccgt
      121 tacgacggtg ttcgctacgg cctgagggtc gaaggcaaag acatcttcca gatgaacgct
      181 cttacgcgat cggccgggtt cggccccgag gtgaagcgtc ggatcatgct tggaacctac
      241 gcgctctctg ccggttacta cgacgcttac tacgggaagg cccagaaagc acggaccttg
      301 atcatcagag atttcgaacg cgcttacgag agatacgact tgctcgtctc acccgcgtct
      361 ccgacgacgg catttcgatt cggcgagaag acgagtgatc ccttgaccat gtatctctcg
      421 gatatctgca atgtccccac ctcgctggcg ggggccgccg cgatatcggt gccatgtgga
      481 acggcgccag aagacgggct tcccgtcggt ctccaattca tgtctcggcc actcgatgag
      541 gttcgcctcc tgaaggttgc gtacgcattc gagcagtctc ttggtttcga ttcgaaacca
      601 ccgatgtggg gggcctgatg gctcacaacc atgtgacgac gatcggtctt gagatccacg
      661 tcgagctcca caccgcgacc aagatgttct gcgcttgccc cgttggattc ggcggggaac
      721 ccaactcgcg cgtatgtccg gtctgcctgg gccatcctgg gacacttccc gttccgaacg
      781 agagggccat cgagttcacg gaaaagatcg ccttggcgct cggatgcgaa gtggcgcgca
      841 ggtcgatctt tcatcgcaag aactacttct atcccgacat gccgaagaac taccagatct
      901 cccagtacga catccccgtc ggatcgcgcg gggtcgtcga ggtcgaggtc gaaggggtca
      961 cccatcgcgt tggtatcact cgcgtccacc tggaagagga cacgggtaag tcgctgcaca
     1021 tgggcggcga gggcaggatc cacggggctg actactcgct cgaagacttc aatcgcgcgg
     1081 ggacgccgtt ggtcgagata gtcaccgaac ccgacatcac gagcgcggag gtcgccaaag
     1141 ccttcgcgca ggaactcaga accatccttg agttcctcga agtttccgat gtgaagatgg
     1201 aagagggctc cctgagggtg gatgccaatg tgtcggtagc acctcctggc gaacgcgggg
     1261 tcaaactcga agtcaagaac atgaactcga tgaggtcctt gtatcgagca ctgctgttcg
     1321 aggaagcgcg tcttcagacc gccctcgaga cgggggagga gatcgtacaa gcgacccggc
     1381 actgggatga gaaagccggc gtcaccagga tcggtcgtac caaggagttc tcggaggatt
     1441 accgctactt ctctgaaccc gacctctgtc cgatcgagcc cagcgtcgag tggttgaggc
     1501 agctgcgaga agagctgcct gagtccccgt ctgaccgacg aaagcgcttc accgatgagg
     1561 ggttgagtgc ctacgatgcc ggagtcctga cctcttccaa agtcatcgcc gatttcttcg
     1621 agagagcgag tggtgtgtcg tccgcagggg caaaacctgt cgcgaactgg atcgcgaacg
     1681 acctcatcgg gctattgtcg gagaggtctg aggatctcag ccaagcgagc atcacgcccg
     1741 aagcgctggc ggctctcgtc gacaaggtcg ccgaaggagc gatctcgaag aaccagggca
     1801 agactgccct tgccgagatg ctcgcaacag gaggctcgct cgatgaggtg atcgagaagg
     1861 gaggccttgc tcaggtctcc gatcgcgccg aactcgaagc agcagttgat gaagcgttga
     1921 gagctaaccc ggaaatcgtc gagcgactga aggcgggaga ccgcaaaccg atgggattcc
     1981 tcatcgggca gatcatcaag gcgaccaaag gcaaaggcaa tccggcgatc atcaacgaga
     2041 tccttaagga gcgcataggg tgaacacctc ggacccgcta ctcgattgga tccgcgacga
     2101 gtatcgatgg gtgcgctcca tgatcgagcg tgacgtactg aagggactat cgagcgaaga
     2161 gataaagacg cagctcccaa cgggcaactc gatcgcctgg gtgatgtggc acttggctcg
     2221 catcgaggac atggctgtga cgtcggtcat aagacgcgag ccccaggtct tcttgtcgtt
     2281 cggatcgaga cttggaacca agcagaccgg aacaggaaac agcttcgggg acgaggagtt
     2341 ggctgagctg acagactcac tcgatgtcgt cgttctcctg gaatactggc gtgagatcgg
     2401 cgtcggcaca cgcgactggc tcgggtcggt cacactcgaa gagttgacca agccgatctc
     2461 attcgaagag ctggccaagg tctttcccga aggcgttgtg gggatccctt cgtacagtgt
     2521 tcgggattgg gatgggaagc ccccggcgta cctgctcaag tggccggtga tcctgcatgg
     2581 ttacatccat atcggagaga tggtgaccat ccgcggatcg ctgcccaaga ccgactaggt
     2641 caggacagcc atgggtaggt tatcgaagtt gatggttgtc gaggacgatg acacccactt
     2701 cgaacgactg cgttccgtcc tctctagcca accggtcagt ttgatcaggg ttcactcagg
     2761 ggacgatcgt tggaggcacc tcgatggcgc tgtcgatgtt ttgctactca ccgtttcttc
     2821 actcaccgat gtgtatgaga tagatcaggt ttcagggagt atcccgatcg tcgtgatgac
     2881 cggacagcag gatcaggaac tcgctctgat agggctcgca cgaggtgcag tcggtcacct
     2941 gttcagggag tcgatcgatg ccgactcgtt gatgcggaca ttgaggtaca gcatcgagcg
     3001 attcagggtt caacgcagcc tcctcgaaac ccaagccaac tacaaggttc tctttgcgaa
     3061 cattcccgtg ccgaagtggg tatatgacga gatgaccctt cgattcctcg ctgtcaacga
     3121 cgcagccgtt gaaacatatg gctacagcga ggcggagttt ctttcgatgt cagtccggga
     3181 tctgctgatc gaggaagacc tgcggcgagg tctggaagag ctgggacgga cgatcgacag
     3241 aggagcactg cccccggagg gggtcgttgc tgagaacgcc gtccggcacc gcgtgcgcag
     3301 cggagccatc atcgatatcg aggtggcgtc gagccatctg tcattcgagg gccgaccagc
     3361 gatcttctgc gccatctccg atgtcacggc ccgacgccga gcagaagcga ggttggcggc
     3421 acagcatgcg gtgacgcgag tgttcgctga agccgaggac ctctcacggg ctggccccca
     3481 gatactccaa gcgatcggcg agagcctcgg ttgggacctt ggggttctgt ggctgatgga
     3541 ccgagttacg cgaggcctgc actgcatctc gctttggaag ggccatcgcg tcttcgctcc
     3601 cgactcggag aggcttacgc gatcggcgcg gctgagtccc cgcgagggca tcccagggac
     3661 ggtatgggct gggcggtcac tccgttggtt cggcgacctc aaggacgatc caaccgagag
     3721 gatcgcggcg gcccacagag agggcttcag atccgctctg gccgtaccgg tgggcgaggg
     3781 gatcccccga ggggtcctgg agttcctgac ccgagagagc agggcccccg agccagatgt
     3841 gatcgccctt gtggagacgg tcgggacgca gctgggcctt tttctcgaaa ggaaagaggc
     3901 caccgcagac ctaagggaac gcgaggtccg gctaagcgcg atccttcgat cgtcccttga
     3961 ttcgatcatc ctgatggatc acagaggtct gatcacagag ttcaacgatt ctgccgagcg
     4021 ggtattcgga ttcaccaggg acgaggctgt cggtcgcctc ttggccgacc tgatcatccc
     4081 cccagatcaa cggaagtcac acagcgaagg gcttcgcagg ttccacgaaa ccggcgagga
     4141 gcatgtcatc gggaagcgta tcgagcgcac tgcactaagg gctgatggct caaccttccc
     4201 gatcgagttg acagtcaccg cgatggatct tcccggcccc gcgatgttcg ttgggtcgat
     4261 ccgcgatctc acaacgcaga agcaagcaac cgctgctcta cgtgtgagcg aggggaagta
     4321 tcggcagctt ctggagcagg cctctgatgg catcgctgtt gccgatgcgc agggccgact
     4381 cgaaacggtg aacccacgga tgtgtcagct gctcgctcag gaggagaagg acctggtcgg
     4441 tcttcgtttc gtcgacctta tcgatctcga ggagggaggg gctggcgccc ttgatgccgt
     4501 ggtcccgggt gagtcagtct tggtcgacgg ctc
//
LOCUS       DATDOZ010000083         4063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1893584, whole genome shotgun sequence.
ACCESSION   DATDOZ010000083 DATDOZ010000000
VERSION     DATDOZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893584"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /gene="sigH"
                     /locus_tag="VI541_01575"
     CDS             <1..531
                     /gene="sigH"
                     /locus_tag="VI541_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HLF69625.1"
                     /translation="AKARSYFLQGGDREDIIQEGMIGLFKAVRDFLPDRQSSFRAFAE
                     LCISRQIITAIKTATRQKHIPLNSYISLHKPIASDDADTRSMMDVLPTLEASDPLEIL
                     LNTADFASLKTCFDEILSGLEGEVLILYLDGRSYQEIAEHLGRHVKSIDNALQRIKRK
                     IEVHLLARKEESEIVQ"
     gene            complement(591..1073)
                     /locus_tag="VI541_01580"
     CDS             complement(591..1073)
                     /locus_tag="VI541_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69626.1"
                     /translation="MSPTSGSEAGLSEMSEVTLDGIGPVRVDMTLEEASSAAGRRIIA
                     GVSIGGESPGDCSFAKPDGGPEGLLFMTIDGRIARVDVANREIKTSEGATIGTEESAL
                     RRMYPGITSKPHAYTDGRYLIHRPDGGQRAIVFETDGSKVTQFRSGREPEVEAVEGCA
                     "
     gene            complement(1160..1450)
                     /locus_tag="VI541_01585"
     CDS             complement(1160..1450)
                     /locus_tag="VI541_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69627.1"
                     /translation="MDRIWSWAVQLGSAADPALALASDEQLSVAMGSVWAAEIGFVLG
                     WLVVPIKPGRVVDPQVLKGEAFNTDGDGFSTSPRIVWLKCLSAPIGEKGARS"
     gene            complement(1456..1998)
                     /locus_tag="VI541_01590"
     CDS             complement(1456..1998)
                     /locus_tag="VI541_01590"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF69628.1"
                     /translation="MRGEIVGHPLDHVALVVRRLEPVLERLDWLEAGPIEAFPSEGTR
                     EVYLGVGSGRLLLMEPTSGQSPYARALAKRGAGLHHLAINVPDLREFLGLVRGWLLVP
                     ACLQDLAQTRTAWLARPGVATLLEVREADRAEGVAVVEQVELPGPLPVDPRPAMLPSN
                     DDGVWLTIAGRRRSVSELTR"
     gene            complement(2184..3077)
                     /locus_tag="VI541_01595"
     CDS             complement(2184..3077)
                     /locus_tag="VI541_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLF69629.1"
                     /translation="MIARTEAEGGESITPEQSEVPPTFYGLIWDGNLIGRAGEPTSQD
                     SPIVSQRVTFKSGDLSLVGYLSKPDGPGPFPTVVWNHGSDPDPGSGPQFASLASMFVP
                     AGYVVFAPMRRGHSDSEGEYIVDAIEREAASFGAAAGQQLATLLLQTSQLDDQLAGLA
                     FVRQQLFVDPARIVVAGWSFGGIQTLLGAESDAGYCAAVSISPAAMMWAGNPDIRARL
                     KTAVIRIRIPVFLIQPPKDASLEPARVLGPKLEQRNKLSRVKIYPAEGPEDEQVHCFG
                     GPKGVHVWASDVLAFLNEVNC"
     gene            complement(3131..3622)
                     /locus_tag="VI541_01600"
     CDS             complement(3131..3622)
                     /locus_tag="VI541_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLF69630.1"
                     /translation="MGGAQKPKVDRPLGYWDTESEAPLPWSWASERLERARNYWVVLK
                     RKNGRAYARPVWGVWLDGAFYFDSSSMDQNLATDPEVSVHLESGDEVVIVDGRAERVS
                     DPGARTVLADAINAKYAWDWSPDRFPAPFVIRPKRAFGWCSDPTGMDRGRLFGESGTR
                     WTF"
BASE COUNT          813 a         1325 c         1161 g          764 t
ORIGIN      
        1 gctaaggccc gctcgtactt ccttcagggc ggagaccgcg aggacatcat ccaggaaggc
       61 atgatcggcc tcttcaaggc ggtacgcgac ttcctcccgg atcgtcagag ttctttcaga
      121 gcgttcgccg agctctgcat cagccgacag atcatcacag ccatcaagac agccactcgt
      181 cagaagcaca tcccattgaa cagctacatc tcacttcaca agccgatcgc gagcgatgac
      241 gctgatactc gttcgatgat ggatgtgctc ccgaccctcg aagcatcgga tcccctcgag
      301 atcctgttga acacagcgga tttcgcttcg ctcaagacgt gcttcgatga gatcctctct
      361 ggtctcgaag gcgaagttct gatcctctac ttggacggtc gctcatacca ggagatcgct
      421 gagcacctcg gacgtcatgt gaagtcgatc gacaatgcac tgcagcggat caagcgcaag
      481 atcgaggtcc acctcttggc ccgcaaggaa gagagcgaga tcgttcagta gctagttagt
      541 tgcggagcga gcccccggtt cggaagtgat ccctgtttcg acgagaaggc tcaggcacat
      601 ccctcaacgg cctcgacttc aggttcgcga cccgagcgga attgggtgac cttagaccca
      661 tcagtctcaa agacgatggc tcgctggcct ccatccgggc ggtggatgag gtaccggcca
      721 tctgtgtacg cgtgcggctt gctcgtgatg ccggggtaca tccgacgcaa cgcgctctcc
      781 tcggtaccga tggtagcccc ttctgaggtc ttgatctctc ggttggcaac atcaacgcgt
      841 gcgatgcgcc cgtctatcgt catgaacaac agaccctcgg gccctccgtc cggcttggcg
      901 aagctgcagt cgcccggcga ctcgccgcct atggacacgc ccgctatgat ccgacgtcct
      961 gctgccgaag atgcctcttc cagagtcatg tcgactctca caggaccgat tccatcaagc
     1021 gtcacctcgg acatctctga gagccccgcc tcagaaccgc ttgtgggcga cacagatggc
     1081 ctcatctctg gccgtggggt cgcctttgaa caagccgtcg acaatagcga ggcaatggcc
     1141 actgcagcga atactctcct cacgatctcg ctcctttctc cccaatgggt gcagacaagc
     1201 atttgagcca aacgattcgt ggcgaagtgc tgaaaccatc gccatccgtg ttgaatgcct
     1261 cgcccttgag aacctgtggg tcgactaccc gcccaggttt gatgggtacg acgagccatc
     1321 cgagaacgaa accgatctca gcggcccaga ccgaacccat cgcaacgcta agctgctcat
     1381 ccgacgccaa ggcgagggct gggtctgctg cagatccgag ttgtaccgcc cacgaccaga
     1441 tccggtccat cgagatcatc gggtcaactc gctgaccgag cggcgacgac ccgcgattgt
     1501 cagccacacc ccatcatcgt tcgagggcag catcgccggg cgtggatcaa cgggcagcgg
     1561 gcccggcagc tccacctgct ctaccacggc caccccctcg gcccgatcag cctcgcgaac
     1621 ctcaagcagc gtcgccactc ccggacgggc tagccacgct gtgcgcgtct gggccaggtc
     1681 ctgcaggcac gctggaacaa gaagccaccc gcgcaccaaa ccgaggaact ctcgcaggtc
     1741 ggggacgttg atcgccaagt gatgcagacc cgcacctcgc ttcgcgagcg cgcgagcgta
     1801 cggactctgc ccgctggttg gctccatgag gagcagtcgc cccgatccaa ctcccaagta
     1861 gacctcccgc gttccttcac tcgggaaggc ctctatcgga ccagcctcca gccaatcgag
     1921 tcgctccaag actggctcca gacggcgaac gacaagagca acgtgatcga gcggatggcc
     1981 cacaatctca cctctcaaga actaggaatc attgtgcgca atcgcacgat aggaatcccc
     2041 taccgtactc ccggtcggta cagaccctgc gcgtcagcgg ccggacgccg aaggcggacg
     2101 gcctctgcac gcgcgaatca tacggagtca ccagtcacca agcctgatgc gcctgtggtg
     2161 caggatggcc cagacgatga cgactagcaa ttgacttcat tcaagaaggc aagcacgtcg
     2221 gaagcccaaa cgtgcacccc cttcggcccg ccgaagcagt gcacctgctc gtcttccggc
     2281 ccctcggcgg ggtagatctt gacgcggctg agcttgttgc gctgttctag tttgggaccg
     2341 aggacgcgcg ccggctcgag gcttgcgtcc ttgggcggct gaatcaggaa taccgggatg
     2401 cgtatccgaa tcacggctgt cttgagtcga gcacgaatgt cgggattgcc agcccacatc
     2461 atcgcggcgg gagaaatcga cacggcagca caatagccgg catcactttc ggcaccaaga
     2521 agtgtttgga tgcctccgaa cgaccacccc gccacgacaa tccgcgcggg gtcgacgaat
     2581 agctgctgtc ttacgaatgc caagccggct agctgatcgt cgagttgcga cgtttggagc
     2641 aacagcgtcg ctagctgctg tcccgcagcg gccccgaaag aagcagcctc gcgttcaatg
     2701 gcgtcgacga tgtactcgcc ttcagaatca ctatggccgc gccgcatggg cgcgaacact
     2761 acgtacccgg ctggtacgaa catcgacgcg agcgaagcga attgaggacc gctgcctggg
     2821 tctgggtcgc ttccatgatt ccagacgacg gttgggaacg gcccaggtcc atctggcttt
     2881 gacagatatc caacgaggct caagtcaccg ctcttaaagg tgacgcgctg actgacaatc
     2941 ggcgagtcct gcgacgttgg ctctcccgcc cttccgatca aattgccatc ccagatcagt
     3001 ccgtaaaacg ttggcggcac ctcgctctgc tcgggggtga tgctctcacc gccctccgcc
     3061 tcggttctcg cgatcatgcc cagggtatcg ccatggacgt cggtcgtcgc cgggatcagt
     3121 tccccggtcc ctagaacgtc cagcgcgtgc cagactcccc gaagagcctg cccctgtcca
     3181 tacccgtcgg gtcgctgcac cagccgaacg cgcgcttcgg gcggatgacg aagggcgcgg
     3241 ggaaccggtc aggagaccag tcccacgcgt acttcgcgtt gatcgcgtcg gccagtaccg
     3301 tgcgggcacc agggtcgctg acgcgctcgg cgcgcccatc gactatcacg acctcatcgc
     3361 cgctttcgag atgcacgctc acttcgggat cggttgcaag gttctgatcc atgctgctgc
     3421 tgtcgaagta gaacgcgccg tcgagccata cgccccacac gggacgcgcg tacgcgcgcc
     3481 cgttcttgcg cttcaggacg acccagtaat tgcgcgcgcg ctcgagccgc tcggatgccc
     3541 acgaccacgg caacggtgcc tccgactcgg tatcccagta ccccaacggc cgatcgacct
     3601 tcggtttctg cgcgccaccc atcacgcctc cgatctccgc cgtttcacgg aatggtcggg
     3661 aactaacaag gatagttcgc ccaacggatt tgccgttgac ccgcccacga aggaggtcgg
     3721 gtccaacggc gggtgtatga atccacggtg atgccttcct cgtaactcag agttccggtt
     3781 acgaccgggg atgactcttc caacggcagt cggtccgagt tttggttcga acctgacttc
     3841 taacatgatc gtctccccgg ccggaaccgg atggcggaag tgactttata ggaggctgtt
     3901 ttcgaacccc acacctgctt gtcctttccg tcttcctaat cgtacgtcgg ccgatgaaag
     3961 gaggccaggt ttgcagtgga tgatggggat cgacacccac aaggagtcgt tcgagatcgg
     4021 ggtggtggac cacagggcga gcgaggtgga cttcggaagg ttc
//
LOCUS       DATDOZ010000084         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1122685, whole genome shotgun sequence.
ACCESSION   DATDOZ010000084 DATDOZ010000000
VERSION     DATDOZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122685"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VI541_01605"
     CDS             complement(<1..271)
                     /locus_tag="VI541_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF69631.1"
                     /translation="MKDLRVLLVDDYSSIRTLLRVVLEDEGYRTSEASNATEAIEAVL
                     KTKPDLIILDLMMPQIDGRYVIEHLRQSGNKTPIIVLTAKTEGLDA"
     gene            376..921
                     /locus_tag="VI541_01610"
     CDS             376..921
                     /locus_tag="VI541_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69632.1"
                     /translation="MRRRVGWVTFVVLVGLGASIGAGLPYLSSRQRAELPRAAEQLRL
                     ATTDVAEIFSCFEGAQDDLFSDSSGASTAQTRGDAARPLNTCTTSRLIARRDGVRLPI
                     SSPLSPRHTREAQKKLRGCISALRGVVTDVARTQEEIRAGIARRRLTGEESFAKAQRE
                     ARRADLLLNEAMSAMARVKAG"
     gene            918..2075
                     /locus_tag="VI541_01615"
     CDS             918..2075
                     /locus_tag="VI541_01615"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HLF69633.1"
                     /translation="MRAPTARDAAGGLRIDLVEASASLGDLGTFLPLAVGLVVINGLD
                     PVATFGVAGLVFIISGLFYRVPVPVQPMKAAAAIAIATHASPATIAAASILLGAVTLL
                     LAATRAVDLVIRLFSKPVIRGLQLGVGLLLILSSLTFIEPAAGSALVPTMVAAGLVLV
                     LLASEGRRVPVALIAVLGAVVWSLSQGGHVPVPSPRLPYLHLPSTRLFWGAFTALVLP
                     QIPLTLGNAVASTASLEREYYADAAKRVTPSRLCLTGGVANLIAGVLGGMPLCHGSGG
                     LSAYRRLGARTGGCNLFIGSCLLGAGAIFGRSAVPLFALIPGAVLGGLLAYTGLRHAL
                     LVRDLRGWPLAVALLMGAVGAATHDLAISMAFGIILTGLIAIVRSRRRVAT"
     gene            complement(2078..2148)
                     /locus_tag="VI541_01620"
     tRNA            complement(2078..2148)
                     /locus_tag="VI541_01620"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2114..2116),aa:Lys,seq:ttt)
     gene            complement(2174..>2868)
                     /locus_tag="VI541_01625"
     CDS             complement(2174..>2868)
                     /locus_tag="VI541_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HLF69634.1"
                     /translation="VKEVAQEQGVYATFMPKPVSGINGSGMHTHMSLFEDDRNAFFDA
                     QDPAHLSKVGRAFLAGVLRHAREICAVTNQWVNSYKRLVPGFEAPVYICWARRNRSAL
                     VRVPMYKPGKEQASRIEARFPDPACNPYLAFSVMLAAGLKGIEQNYELPPEADDNIYQ
                     MSPAERTRNGIDSLPEDLHEAIQICESSDLVRETLGEHTFEWFIRNKKQEWDDHKAYV
                     TPYEIERYLPIL"
BASE COUNT          437 a          913 c          906 g          612 t
ORIGIN      
        1 cagcgtcgag gccctcggtc ttggcggtga ggacgatgat cggcgtcttg ttccccgact
       61 ggcgcagatg ctcgatcaca tagcgcccat cgatctgcgg catcatcagg tcgaggatga
      121 tgaggtcggg cttggtctta agcaccgctt cgatcgcctc ggttgcgttg ctcgcctcgg
      181 acgttctgta gccctcatcc tcgagaacca cccgaagcag cgtccttata gaggagtagt
      241 catcgacgag gagcacccga agatccttca cggagtaagt ctatgccgat ggcccccgga
      301 cgtcgcggca tggtgcgcga cgacatcggg ggctatcgtc tcttctgaca tgggcaatcg
      361 ggcaggtcgc atcagatgag gcgacgcgtc ggatgggtca ccttcgtggt gctcgtgggt
      421 ctgggggcct cgatcggcgc agggctcccc tatctctcca gccgacagcg agcggagctg
      481 cctcgggcgg ccgagcaact tcggctcgct acaaccgatg tcgccgagat cttcagctgt
      541 ttcgagggag cgcaagacga tctgttcagc gactcttcgg gtgcatccac cgcccagact
      601 cgtggggatg cagcgcgccc tcttaacacc tgcaccacat ccagattgat cgcgcgccgc
      661 gatggcgtgc ggttaccgat ctcatcaccg ctctcgccgc gccacacccg cgaagcccag
      721 aagaagctaa gggggtgcat ctccgccctc aggggagtcg taaccgacgt cgctcgcacg
      781 caggaggaga tccgcgccgg gatcgcaagg cggcgtctca ctggggagga atcgtttgcc
      841 aaggctcagc gtgaggctcg ccgagccgat ctgctactga atgaggccat gagcgcgatg
      901 gccagggtga aggccggatg agggcgccga ccgcacggga tgccgcaggc ggacttcgca
      961 tcgatctcgt tgaggccagt gcctcgctcg gggacctcgg gacgttcctt ccacttgcgg
     1021 tgggccttgt agtgatcaac gggctggatc ctgttgccac attcggtgtt gcgggcctcg
     1081 tcttcatcat cagcggtctg ttctatcgag tgcccgtccc ggttcagccc atgaaggcgg
     1141 cggcggccat agcgatcgcc acacatgctt cgcctgcgac tatcgcggcg gcttcgatcc
     1201 tgctcggcgc ggtgaccctc ttactcgcag ccacccgcgc ggtggatctt gtgatccggc
     1261 tgttctcgaa gccggtgatc cgtgggcttc aactgggtgt tggcctgctt ctgatcctct
     1321 cgtcactcac cttcatcgaa ccggccgctg gttcggcgct cgtcccgacc atggtggccg
     1381 ccggcctcgt cctcgtcctt ctcgcctctg agggccgacg tgtgcccgtc gctctcatcg
     1441 cggtcttggg tgccgttgta tggagtctct ctcagggtgg gcatgtccct gtcccatccc
     1501 ctcgcctgcc ctatcttcat cttccttcga cgcgcctgtt ctggggcgcg ttcacggcgt
     1561 tggtgcttcc gcagatcccg ctcactctgg gcaacgcggt ggccagcacc gcgtcgttgg
     1621 agcgcgagta ctacgcagat gcggcgaagc gggtcacccc ttcgcgcctg tgcctcaccg
     1681 gtggagtcgc caatctcatc gccggtgtct tgggcgggat gcccctgtgc cacggctcgg
     1741 gcggcctcag cgcttatcga agattggggg cgcgcaccgg tggatgcaac ctgttcatcg
     1801 gatcctgcct gttgggggcc ggggccatct tcggccggtc agcggttccc ctgtttgctc
     1861 tgataccggg ggcagttctc ggcggccttc ttgcctacac agggctccgt catgcgctcc
     1921 tcgtacgcga tctgcggggc tggccgctgg cggttgcact gctcatgggg gccgtcggcg
     1981 cggctaccca cgatctcgcg atctcgatgg catttgggat catcttgacc ggtctcatcg
     2041 ccatcgttcg ttccagacga cgcgtcgcga cttagtgtgg gcccccaggg aatcgaaccc
     2101 tggtcgacgg attaaaagtc cgcagcttta ccactaagct aggggcccgc ccggaagtgt
     2161 accgaccgca gtcctagagg atgggtaggt aacgctcgat ctcgtatggg gtcacgtagg
     2221 ccttgtggtc gtcccactcc tgcttcttgt ttcggatgaa ccactcgaag gtatgctcgc
     2281 cgagcgtctc gcgaacgaga tcggatgact cacatatctg gatcgcctca tgcagatcct
     2341 cggggagtga gtcgatgccg ttccgcgttc gctcagccgg ggacatctga tagatgttgt
     2401 cgtcggcctc cggtggcagt tcgtagttct gttcgatgcc tttcagcccg gctgcgagca
     2461 tcaccgagaa cgccaggtat gggttgcacg cggggtctgg gaaccgtgct tcgatccgcg
     2521 acgcctgctc cttgcccggc ttgtacatcg ggacccgcac caacgccgac cggttacgcc
     2581 gggcccagca gatgtagacg ggggcctcga accctgggac cagtcgcttg tacgagttga
     2641 cccattggtt tgtgaccgcg cagatctctc gcgcatgcct aagcactccc gccaggaatg
     2701 ctcggccgac cttggagaga tgggccggat cctgcgcgtc gaagaaggcg ttgcggtcgt
     2761 cctcgaagag cgacatgtga gtgtgcatgc cgcttccgtt gatcccagag accggtttcg
     2821 gcatgaaggt cgcgtacaca ccctgttcct gtgcgacctc cttgacca
//
LOCUS       DATDOZ010000085         5582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3216578, whole genome shotgun sequence.
ACCESSION   DATDOZ010000085 DATDOZ010000000
VERSION     DATDOZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216578"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VI541_01630"
     CDS             complement(<1..240)
                     /locus_tag="VI541_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLF69635.1"
                     /translation="MRSLDPKDQNWYQRAVFYEVLVRGFHDSNGDGSGDLRGLIGKLD
                     YLEWLGIDCIWLLPFYRSPLRDGGYDISDFFSVLPE"
     gene            complement(237..2240)
                     /locus_tag="VI541_01635"
     CDS             complement(237..2240)
                     /locus_tag="VI541_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HLF69636.1"
                     /translation="MEPSSRRRAPISRDRPRLRSVAAAPTPPVRVFIEGIRPSTPAGY
                     PAKAVAQRNTEISADILCDGHELLVAWARWRRTGGASLHSVPLEHLANDRWEGHLVPD
                     RVGLHEFYIEAGVDLYATWRQEILAKHSAGDDVSVELKEGVALLQRAATGGPNVGHRR
                     LLLAAARDMGDAELTTAKRVRAGTGVQIRGAMMRSPLVAHITRSDQMSLWVDRERAAF
                     GAWYEMFPRSEGGFAGTMRRLPAIARMGFDVVYLAPIHPIGTTKRKGPNNSEEGGTES
                     VGSPWAIGAQGGGHMSIDPNLGTLTEFRALLSRARDLDLEVAMDFALQCSPDHPWVTQ
                     HPEWFNHRPDGSIKHAENPPKKYQDIYPLNFWPPHDEDRRRLWEACKQILDYWIAKGV
                     KIFRVDNPHTKPISFWAWLIREIQARHPDVIFLSEAFTRPKVMSKLAEVGFTQSYTYF
                     AWRNSKKELTTYLTELAGPRSDYLRPNFWTNTPDILSGPLRFGGPAAFKMRLVLAATM
                     SPSYGMYSGYELLENVPASEENEEYQDSEKYEIKRRNHSSESSIADFVARVNRIRRRH
                     AAFRDVTNVRFHHAEHHNVLVYSKSSDDLRDVMLMVVNLDPNEPVESALWLNLELLGL
                     PWDRSYVAYDELDGAKYVWESSQPNVRLDPAERVAHIMHMRRR"
     gene            2239..4761
                     /gene="glgP"
                     /locus_tag="VI541_01640"
     CDS             2239..4761
                     /gene="glgP"
                     /locus_tag="VI541_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HLF69637.1"
                     /translation="MQPKASRTFNVRAQLPPSLIRLHDIAMNLRWSWDPRSEDLFRWV
                     DQEAWEATGHDPIRVLAGLTQERIESLIADPGFMEFLERVGDDLDRYMETPAWYQKRA
                     GLLRRVAYFSPEYGISEAVPQYSGGLGVLAGDHLKAASSLGLPMVGVGLFYKEGYFRQ
                     QLNADGWQEEHYPTLDPANMALSSCGTQIKIDMAGTSVVAQVWLSQVGRIPLYLLDTD
                     LSQNPDEFRTVTDRLYGGGEEHRLQQEILLGAGGVRALDAMGVKPQVFHMNEGHAGFL
                     ALERIRQAIVDHGLEFSEAIEAVRATTAFTTHTPVPAGIDQFSRPLMEKYFSSWAEET
                     GVPFDRLMALGQHNGSGDGPFNMAIMCLRLSGRANGVSELHSAVTRQMFQHLWPNSPM
                     EEVPIKSITNGVHPRTWIAPGMSELFDRYVLPEWNEAGPQRWAHIYDARDDELWRLRL
                     QNREALVTFVRDRMRKISLAAGASSLDLDWIDNVLDPRVLTIGFARRFAEYKRATLLL
                     TQPDRLRSLLMSDTPIQIVFAGKAHPADNEGKQMIRQLVQFCRDPAVRHRIVFIEGYD
                     ISVARALYQGCDVWLNTPRRPLEACGTSGEKAALNGALNVSTLDGWWDEMFDGENGWA
                     ISSAEDYEDLGRRDEVEAASLFDLLERHIIPRFYQRSESGIPTAWIQRIKASLASLGP
                     KVSASRMVRDYVIQMYEPLGELTDALSADGYSRARKLAAWKARVRETWDGVKIRSFDS
                     EDGSAALGSSRVVEASVELGALSPEDVEVQIVHGAVGPNGDLDRTSIAVMELQGIDGD
                     ASMRYRGEFPCDLSGKYGFAVRVVPRHTDMSTYAELGCITWA"
     gene            complement(4748..>5582)
                     /locus_tag="VI541_01645"
     CDS             complement(4748..>5582)
                     /locus_tag="VI541_01645"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLF69638.1"
                     /translation="DTSVGAPPDKFNTQGQDWSTPPFDPWALREGGYEPFIQTLRATM
                     RASRGLRIDHVMGLARLYCIPKGSSAAQGAYVRYPVDDLMAIVALESRRARAFVVGED
                     LGTVEGSLRAKMTDQQMLSYRLVIFSGFSASRYPRMTFAAVSTHDLPTIAGLWSGSDL
                     LEQSERGLSPNGPATAAMISSIRKGLKIRRGLKLREASVELVADRIHSWLAERDSSVA
                     VVSLEDLLGEPSRVNMPSTHLKRPNWSMALSSTLEDLTEDPRARRLSDQMRNRRGPMP
                     R"
BASE COUNT         1091 a         1669 c         1710 g         1112 t
ORIGIN      
        1 ctcaggcagc acgctgaaga agtcgctgat gtcgtagccg ccgtctctca ggggcgagcg
       61 gtagaagggc aggagccata tgcagtcgat ccccagccat tcgagatagt cgagcttacc
      121 gatgagaccg cgcagatccc ccgaaccgtc gccattggag tcgtggaaac cgcggactag
      181 tacctcgtag aagacggctc tctgatacca gttctgatcc ttcggatcca gggatctcat
      241 cgtcgcctca tatgcatgat gtgagcaacc ctctccgccg ggtcgagacg gacattcggc
      301 tggctgcttt cccagacata cttcgcacca tccaattcat cgtacgccac gtaactgcga
      361 tcccagggaa gtcccagtag ttctaggttc agccaaagtg cggactcgac gggttcgttc
      421 ggatcgaggt tgaccaccat gagcatgacg tcacgaagat cgtcggatga cttcgagtac
      481 accaagacgt tgtgatgttc cgcatgatgg aaccggacgt tggtcacgtc ccggaacgcg
      541 gcgtgtctgc gtctgatgcg gttgaccctc gcgacgaagt cagcgatcga cgactcgctg
      601 gagtggttcc gtcgcttgat ctcgtacttc tccgagtcct ggtactcctc gttctcttcc
      661 gaggctggaa cattctcgag aagctcgtac ccgctataca tcccatagct cggactcatg
      721 gttgcagcca gaacgaggcg catcttgaag gctgctgggc ccccgaatcg caacgggcca
      781 cttaggatgt cgggcgtgtt ggtccagaag tttggccgca gataatcact gcgggggcca
      841 gccagttccg tcagatacgt cgtgagttcc ttcttggagt tgcgccacgc gaagtaggtg
      901 tagctctgag tgaaccccac ctcggctagc ttgctcatca ccttggggcg tgtgaaggct
      961 tcggatagga agatcacgtc ggggtggcgc gcctgtatct cccggatgag ccaagcccag
     1021 aaagagatgg gcttcgtgtg aggattgtcg acccggaaga tcttcacacc tttggcgatc
     1081 cagtagtcga ggatctgttt gcatgcctcc cagagccgac gacggtcctc gtcatggggg
     1141 ggccagaagt tcaacgggta gatgtcctga tacttcttcg gtggattctc agcatgcttg
     1201 atcgatccat cggggcggtg gttgaaccac tcggggtgtt gcgttaccca cggatgatcc
     1261 ggtgagcatt gaagggcgaa gtccatcgcg acctccaagt cgaggtcccg tgcccgactt
     1321 agcaacgccc gaaattcggt caacgtaccg aggttcggat cgatgctcat gtgtcctccc
     1381 ccttgggcac cgatcgccca cgggctccct accgactccg tgccaccttc ctcgctgttg
     1441 tttgggccct tccgcttggt ggtccctatc ggatggatcg gcgcgaggta cacgacgtcg
     1501 aaccccatcc ttgctatcgc ggggagtctt cgcatcgttc cggcgaaccc gccctccgac
     1561 cgcgggaaca tctcgtacca cgcgccgaac gcggctctct cgcggtcgac ccaaagagac
     1621 atctggtcgg accgtgtgat gtgcgcgacc agcggagatc gcatcatggc tccgcggatc
     1681 tgcacccccg taccggcacg aacgcgcttc gccgtagtca gttctgcgtc tcccatgtcc
     1741 ctagccgcag cgagaagcag tcgacggtgc ccaacgttgg gccccccggt tgccgctcgc
     1801 tgtaagagcg ccaccccctc cttgagttcg accgaaacat cgtcgccagc agagtgtttg
     1861 gccaggatct cctgcctcca tgtggcatag agatcgaccc cggcctcgat atagaactcg
     1921 tgcagcccga cccgatccgg tacgaggtga ccctcccacc ggtcgtttgc aagatgctca
     1981 agtgggaccg aatgaaggga tgcacccccg gttcggcgcc aacgagccca ggcgacgaga
     2041 agttcatgcc cgtcgcagag gatatcggcg gatatctccg tgttgcgttg tgcaactgcc
     2101 ttcgcgggat acccggcagg ggtgctgggg cggatcccct cgatgaacac ccggaccgga
     2161 ggggtcggtg ccgctgcgac gctccgaagg cgcggcctat cccggctgat gggtgctctc
     2221 ctgcgcgatg atggctccat gcagcctaag gctagtcgaa ccttcaacgt tcgggcccaa
     2281 ttaccgccat ctctcatacg acttcatgac atagcgatga acttgaggtg gtcctgggat
     2341 ccgcgcagcg aggacctctt ccggtgggtc gatcaggagg cctgggaggc gaccgggcac
     2401 gatccgatac gcgtgctcgc tggcttgacc caagaacgca tcgagagcct catcgcagat
     2461 ccgggattca tggagttcct cgaaagggtc ggcgacgatc tcgatcggta catggagacg
     2521 cctgcctggt accagaaacg cgcagggttg ctccgacgcg tcgcttactt ctcgcccgag
     2581 tacgggatct ccgaggctgt cccccagtat tcggggggcc tgggggtcct ggcgggtgac
     2641 cacctgaagg ccgcgagcag tctgggactg ccgatggtcg gagtcggact cttctataag
     2701 gaaggctact tccgccagca actgaatgcc gacggctggc aggaggagca ctaccccacc
     2761 ctcgacccgg cgaacatggc attgagttca tgcggcactc agataaagat cgacatggcc
     2821 ggaacgtctg tcgttgcgca ggtctggctg agccaggttg ggcgcatccc tctctatctg
     2881 ctcgatacag atctgagcca gaacccggat gagttccgga cggttaccga caggctctat
     2941 ggcggtggag aggagcatcg acttcagcag gagatcctcc tgggggccgg gggtgttcgt
     3001 gctctcgatg ccatgggggt caaacctcag gtcttccata tgaatgaggg ccacgcaggc
     3061 ttcctcgctc tagaacgcat acgacaagcg atcgtagatc acggactgga gttcagcgag
     3121 gccatcgagg cggtccgtgc cacgacggcg ttcactaccc acaccccggt cccagcgggc
     3181 atcgaccagt tctcacgacc gctgatggag aagtacttct catcatgggc agaagaaact
     3241 ggtgtgccgt tcgatcgcct gatggccctg ggccagcaca acggatccgg cgacggtccc
     3301 ttcaacatgg cgatcatgtg cctgcgcttg tcgggaaggg ccaatggtgt ctccgaacta
     3361 cacagcgccg tcacgcgcca gatgtttcaa catctatggc cgaacagccc aatggaagag
     3421 gtaccgatca aatcgataac caacggcgta cacccgagga cctggatcgc tcccgggatg
     3481 tctgaactct tcgatagata cgtcctgcct gagtggaatg aagctggtcc gcagagatgg
     3541 gcacacatct acgatgcgag ggatgacgaa ctctggagat tgcggcttca gaaccgcgag
     3601 gccctggtca ccttcgttcg ggatcggatg aggaagatat cccttgcggc aggggccagc
     3661 tctctggatc tcgactggat cgataacgtg ctggaccctc gcgtcttgac gatcggcttt
     3721 gcgagaaggt tcgccgagta caagcgagcc acgcttctgc tcacgcagcc ggaccgactt
     3781 cgatcactgt tgatgagcga caccccgata cagatcgtct tcgcgggcaa ggctcatccc
     3841 gccgacaacg aaggcaaaca gatgatccgc cagctcgtgc agttctgtcg cgaccctgca
     3901 gttcgacacc gcatcgtatt catcgagggc tacgacatct cagtggcgag ggccctctat
     3961 caggggtgcg acgtctggct gaacacgcca cggcgtcccc tcgaggcctg cggcacgagc
     4021 ggagagaagg cggcactgaa tggggctctc aacgtatcga ccctcgatgg gtggtgggat
     4081 gagatgttcg atggtgagaa cggctgggcg atctcctcgg ccgaggacta cgaagatctc
     4141 ggtcgcaggg acgaggtcga ggcggcctcg ctattcgacc tgctggaacg gcacatcatc
     4201 ccccgcttct accaacgctc ggagtctggg ataccaacag catggatcca acggatcaag
     4261 gcatctctcg catctttggg gccaaaggtg agtgcgtctc gcatggttcg cgactacgtg
     4321 atccagatgt acgaacctct cggcgaactg accgatgctc tatcggctga tggctattcg
     4381 agggcgcgca agcttgcggc ttggaaggct cgggtgcgcg agacgtggga cggggtcaag
     4441 atccgttcct tcgattccga ggacggatcc gccgcgctcg gttcctcgcg cgtggtcgag
     4501 gcatcggtcg agttgggggc cttatctccc gaagatgtgg aggtccagat cgtgcacggc
     4561 gcggtcgggc cgaacggaga tctagataga acgtcgatcg cggtcatgga actgcagggg
     4621 atcgatggtg acgcatcgat gcgttaccgc ggagagttcc cgtgcgatct gtccgggaag
     4681 tacggattcg ccgtgcgggt ggttccgagg cacaccgaca tgagcacgta tgcagaactt
     4741 ggctgcatca cctgggcata gggcccctgc gatttcgcat ctggtccgat agacgtcggg
     4801 ctctcgggtc ttcggtcaga tcctccagcg tcgagctcaa ggccatcgac caattcggcc
     4861 ttttcagatg cgtcgaaggc atgttgaccc tcgacggttc gcccaataga tcctcgagac
     4921 ttacgacggc gacactcgag tcgcgctcgg ctagccatga gtggatgcga tcggccacga
     4981 gctcgaccga ggcctccctg agtttcaagc ccctgcggat cttcagacct ttgcggatcg
     5041 atgagatcat cgccgcagtc gctggtccat tgggagacag gccgcgctcg ctctgctcta
     5101 gcaggtccga ccctgaccag aggccagcga tggtcggcag atcgtgtgtg gagaccgctg
     5161 cgaaggtcat acgcggatag cgcgacgcag agaagccgct gaatatgacc aaacgatagg
     5221 agagcatctg ctggtcggtc atcttggcgc gaagcgatcc ttcgacggtt cccagatctt
     5281 cgcccaccac aaatgcccgg gcccgccgac tctcgagtgc gacgatagcc atcaggtcat
     5341 cgaccgggta acgaacatag gcaccctgcg ccgcgctgga acccttcggg atgcagtaga
     5401 gcctggcgag gcccatgacg tggtcgatgc gaagtccacg agatgctctc atcgttgctc
     5461 gtagggtctg gatgaatggc tcgtacccgc cttcgcgaag agcccacgga tcgaaaggcg
     5521 gcgtggacca atcctgcccc tgagtattga acttgtccgg aggggccccc acactcgtat
     5581 cg
//
LOCUS       DATDOZ010000086         4001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3740647, whole genome shotgun sequence.
ACCESSION   DATDOZ010000086 DATDOZ010000000
VERSION     DATDOZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3740647"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..640
                     /locus_tag="VI541_01650"
     CDS             155..640
                     /locus_tag="VI541_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69639.1"
                     /translation="MITTKQTASIGASPDSIALVMRRPASIAAFPGIDSATPGAPGYR
                     LEVEVAPLGFPMRDRVWLAWGLPHHSDDAVQYPFTIEGGRWLTGAGSARIAQRAHNVS
                     DVTITWELDPPRLLLAIPLMKPAMRRCLRLATRRWLRTVRVAAERPSAARSLTLADVG
                     A"
     gene            complement(665..1669)
                     /locus_tag="VI541_01655"
     CDS             complement(665..1669)
                     /locus_tag="VI541_01655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLF69640.1"
                     /translation="MRIGMVVGGPMQGVGAVAAAIEGAGYDSVWTAETGSDAFIQAAI
                     VAAATTRVRVGTAIALAFPRSPGIAAMTALDLDELSGGRFICGLGSQVKRVNEERFST
                     PFEHPAPKMRDYILAMRAFIGGHFGESPEHEGRFYRVTMAPWPRMSPPLRRDIPIYLA
                     AVNEQMQRVAGQVADGVVGHPMTSIDYIKQVVLPNIAKGAAAAGRKASDIELAQQVSI
                     SISSDRAVALNEVKQQIGFYATTRTYTPVLALHGFDEVVPDLRAAFAEKDMARLAALV
                     SDDMADTFAIYGTPDEVIEKARRFEGVVGELTLGTPWYRVDRVRMLENYQLIMSTFAR
                     "
     gene            complement(1870..2073)
                     /locus_tag="VI541_01660"
     CDS             complement(1870..2073)
                     /locus_tag="VI541_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69641.1"
                     /translation="MRVLLYTCQECGSRIVTFENIFEEVCSNCGAIASAEAGLRITIS
                     LAMEDFPELQREIRTEPRLVVHV"
     gene            complement(2196..2684)
                     /locus_tag="VI541_01665"
     CDS             complement(2196..2684)
                     /locus_tag="VI541_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HLF69642.1"
                     /translation="MAINERTRADWDTYFLGLAAAAASRSSCTQDKHGAVMVSKRRIR
                     STGYNGTPTGYGHCDEGACPRGKKGDPTKPCWGLHAEANALLYTGPEEREGATLYVTC
                     PPCIECAKLIANSGLREVVAQEGPSEDLEKVRRFLLDCKVRFRILGGDGTGQLKVRPK
                     QR"
     gene            complement(2706..3509)
                     /locus_tag="VI541_01670"
     CDS             complement(2706..3509)
                     /locus_tag="VI541_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLF69643.1"
                     /translation="MNWKGKVCVITGASSGFGESVARELASRGARIIAVARREEKLRE
                     IIDDVGGAPHSYIKADISDLAQVRAMATRLAQLTDHVDVLINNAGVGTSGPLAQATSE
                     ELEGVIRTNLLGAIWCTKESLALIEKAPRERRTPIVVNVASMAGRIALPGSADYAASK
                     FGLVGFTESIWYELSAKGIRTMMVNPGLADTEGFPMDAIRAKPLLGWSVMDASRVAGA
                     LVRGIEQGSFEVRVQWWMNPIYYLVLVMGPLRRTVVGALRRSVGNVGKF"
     gene            3544..>4001
                     /locus_tag="VI541_01675"
     CDS             3544..>4001
                     /locus_tag="VI541_01675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLF69644.1"
                     /translation="MRTIVHLVRHAEVHNPGNIWYGRLEGFHLSERGLRQASALAEFF
                     SARPVSAVYCSPLQRALETATPIAAARALEVVIDEALIESMTKLQGGPADKRIFRNPL
                     NLRYFINPFRPTWGEPYASTGARMVGAITRMRELHRGGEVVAVSHMTPVLV"
BASE COUNT          719 a         1297 c         1216 g          769 t
ORIGIN      
        1 ggcctccggt gatgcgaagc ttgcagccag attcgtcacc ttcttcgaca gaggtcatgg
       61 caccctatag gttctgtatc tccgttacta gattttttca tgtaacggcg gtaagatcac
      121 ctgggtgagt caccgccaac aggaggactc aaagatgatc acaaccaagc agacggcgtc
      181 gataggagca tcaccggaca gcatcgcact tgtcatgcgc cgtcccgcct cgatcgccgc
      241 cttccctggg atcgattccg cgactccggg ggccccgggc tatcgtctcg aagtcgaggt
      301 cgctcctctg ggtttcccga tgcgcgaccg ggtctggctc gcatggggtc tcccccacca
      361 ctcggacgac gcagtccagt atccgttcac catcgagggc gggcgttggc tcacgggggc
      421 cggctctgcg cgcatcgcac aacgcgctca caacgtcagc gacgtgacga tcacctggga
      481 actcgacccg ccgagactgc tgctggccat ccctctgatg aagccagcca tgcgcaggtg
      541 cttgcggctc gctactcgcc gctggttgag gacggtccgg gtcgctgcag agcgaccgtc
      601 ggccgcaagg tcgttgactc tcgcggacgt gggggcctga gactgcgggg gctttcgggc
      661 ggcgctaccg cgcgaacgtc gacatgatca actggtagtt ctctagcatg cgcacccgat
      721 cgaccctgta ccacggggtc ccaagggtca gttcgcccac taccccttcg aaacgcctcg
      781 ctttctcgat gacctcgtcg ggcgttccgt agatggcgaa ggtgtcggcc atgtcgtcgc
      841 tcacgagcgc agcgagtcgc gccatgtctt tctccgcgaa ggccgcgcgc agatcgggga
      901 cgacctcgtc gaatccatgc agcgccagca cgggtgtgta cgtgcgcgtc gtcgcataga
      961 agccgatctg ctgcttgacc tcgttcagcg cgaccgcgcg gtctgatgag atcgagatgc
     1021 tcacctgctg cgccaactcg atgtcggaag ccttgcgtcc cgcagccgcg gccccctttg
     1081 ctatgttggg gagaaccacc tgcttgatgt agtcgatcga ggtcatcggg tgaccgacaa
     1141 ctccatcagc gacctgtcct gcaacgcgct gcatctgttc gttgaccgct gcgaggtaga
     1201 tagggatgtc gcgccgaagg ggtggggaca tgcgcggcca cggcgccatg gtcacgcggt
     1261 agaagcgacc ctcatgttcg ggactctcgc cgaagtggcc cccgatgaag gccctcatcg
     1321 cgaggatgta gtcgcgcatc ttgggggccg gatgctcgaa cggcgtcgag aatcgctcct
     1381 cgttgacgcg cttgacctgg ctccccaacc cgcagatgaa gcgaccaccg gagagctcat
     1441 cgaggtcgag tgcggtcatc gccgctatcc ctgggcttct cggaaacgct agggcgatag
     1501 ctgtaccgac cctgacccgt gtcgtcgccg cagcgacgat cgccgcctgg atgaaggcat
     1561 cggagccggt ctcggctgtc cacaccgagt cgtagcccgc gccctctatg gccgcagcga
     1621 ccgctcccac gccctgcatg ggtccaccga ccaccatccc gatgcgcatg agccaagcct
     1681 atatcttggg caggactcga acatatgttc tatcatgggg gcatggcaga ttcacaactc
     1741 gccctggctg cgggccctaa accgaagacg gatcagtcgt ccaagcagat gaccttcgag
     1801 gtgcgcccga tcaaggttag gcgcgcgccg aagtgggtca aggagccttt ggttaagggc
     1861 gcctcgcgat cagacgtgca ctactagtct tggctcggtc ctgatctcgc gctgcagttc
     1921 agggaaatcc tccatcgcga gggatatcgt gatccgaaga cctgcttcag cagaggcgat
     1981 agctccgcag ttggaacaga cctcttcgaa tatgttctcg aatgtgacta tgcggctgcc
     2041 acactcttgg catgtatata ggagcaccct caccctcctt ccctcgacct gactgacctt
     2101 acgacggcgc gaggaggatg tgaatagccc tttgtgcggt tcttggtgat tcggtcagat
     2161 aacgcgcagc gtgcacggac atactgaccc acaggtcatc tttgcttggg tctgaccttc
     2221 aactggcccg ttccgtcacc accgaggatc cggaatctca ccttgcagtc gagcaggaat
     2281 cggcgcacct tttcgaggtc ctctgaagga ccttcctgcg cgacgacctc gcgcagcccc
     2341 gagttggcga tcagcttcgc gcactcgata catggagggc aggtgacata gagagtcgct
     2401 ccctcgcgtt cctccgggcc cgtgtagaga agagcgttcg cctcggcgtg gagaccccag
     2461 catggctttg tggggtcgcc cttcttacca cgaggacagg ccccctcatc gcagtggccg
     2521 tatcccgtgg gcgtaccgtt gtaaccggta gaacggatgc ggcgcttcga gaccatcacc
     2581 gcgccatgct tgtcctgcgt gcaactcgaa cggcttgccg cggccgcggc gagacccagg
     2641 aagtaggtgt cccagtccgc gcgtgttcgc tcgttgatcg ccatcggtgg cgacatcata
     2701 cggaatcaga acttgccgac gttacccacg ctgcgacgca gggcccccac aaccgttcgg
     2761 cgaagggggc ccatcacaag aaccaggtaa tagatcgggt tcatccacca ctggacccgc
     2821 acctcgaatg acccctgctc gatcccgcgt acgagggccc cggcgactcg gctcgcatcc
     2881 atcaccgacc acccgagcag gggcttggcg cgtatcgcgt ccatcgggaa gccttcggtg
     2941 tcggcgagcc cagggttgac catcatcgtc ctgatgccct tggccgatag ctcgtaccag
     3001 atcgactcgg tgaagccgac gagcccgaac ttcgaggccg cgtagtcggc ggagccgggc
     3061 agcgcgatcc gcccggccat cgaagccacg ttgacgacga tcggagttcg ccgttcgcgg
     3121 ggggccttct cgatgagtgc gagcgactcc ttcgtgcacc agatcgcacc gagaagattc
     3181 gtacggatca ctccttcgag ttcctcggat gtggcctgtg ccagcggacc actggtgccc
     3241 acgccagcgt tgttgatgag gacgtcaacg tggtcagtca gttgagcgag tcgggtggcc
     3301 atcgctcgca cctgcgcgag gtcggagata tccgccttta tgtaactgtg gggcgctccc
     3361 ccgacatcat cgatgatctc tcggagcttc tcctctcggc gcgcaacagc gatgatgcgg
     3421 gcgccgcgcg aggccagttc acgcgcgacc gactcgccga acccgctcga agcccccgtg
     3481 atgacgcaga ccttgccctt ccagttcatt ggggcatcct gacatcgttt accctttgcg
     3541 cgcatgagaa ctatcgttca cctggtgcga cacgccgagg tccataaccc cggcaacatc
     3601 tggtatggaa ggctcgaagg gttccacctc tccgagcgag gactgcggca ggccagcgcc
     3661 ttggccgagt tcttctcggc gcgaccggtc tcggcggtgt actgcagtcc acttcagagg
     3721 gctctcgaga ccgcaacacc gatcgcagcg gccagggccc tcgaggtagt gatcgatgaa
     3781 gccctgatcg agtcgatgac caagttgcag gggggccccg ccgacaaacg catctttcgc
     3841 aaccccctca acctgcgcta cttcatcaac ccgtttcgcc cgacgtgggg cgagccctac
     3901 gcgagcacgg gggcccggat ggtcggagcg atcacacgga tgcgcgaact gcatcgcggt
     3961 ggcgaggtcg tggcggtatc gcatatgacg ccggtccttg t
//
LOCUS       DATDOZ010000087         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2684316, whole genome shotgun sequence.
ACCESSION   DATDOZ010000087 DATDOZ010000000
VERSION     DATDOZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684316"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="VI541_01680"
     CDS             complement(<1..1740)
                     /locus_tag="VI541_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478181.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HLF69645.1"
                     /translation="MERYRPKHPIRIVTAASLFDGHDAAINIMRRILQGSGAEVIHLG
                     HNRSAAEIVECAIHEDAQAIAVTSYQGGHLEFFSYMHQLLEERGANIKIFAGGGGTIL
                     PEEIEALHAAGIARVYSPDDGRSMGLQGMIDDLLKTADFPTRPPGLDGHLARLDPHDP
                     STIAGLITLAENYPELRDDLARQLEPLVASRTTPVLGITGTGGAGKSSVVDELIRRFL
                     ADFPEKTVAILSVDPSKRKSGGALLGDRIRMNSIFTPRVYMRSLATRGENLSLSRQVT
                     EAIAIVKAAGFDFVVVETSGIGQSDTQIVDHADVSLYVMTADYGAATQLEKIDMLDFA
                     DVIAINKFDKPGSLDALRDVRKQYRRNHGGTDLPEAEIPIVGTIASQFNDPGMNDLYR
                     LVMEVLARKTGAPFGGEVSAHERLTAQRVAIIPAARIRYLAEIAESLDAERTWIRQQA
                     EIARQLYQLHGAVRALRKEVGPDEALVPEFERQQDDPPSVDALIGEYEALRTRLDPRS
                     VTLLTAWPAMRASYREDLYRYTVRDRVIERELFTESLSHLRIPKIALPRYSDWGDLLT
                     WLLEENVPGEFPFT"
BASE COUNT          390 a          605 c          650 g          402 t
ORIGIN      
        1 ggtgaagggg aactccccgg ggacattctc ctcgagcaac caggtgagca gatcccccca
       61 gtcgctgtac cgcggcaagg cgattttcgg gatgcgaaga tgggacagag actcagtgaa
      121 cagttcccgc tcgatcacgc gatcgcgaac cgtgtaacgg tacaggtctt cgcggtagga
      181 cgctcgcatt gcgggccagg ccgtcaggag ggtcacgctg cggggatcga gtcgggtgcg
      241 aagcgcctca tactctccga tgagggcgtc taccgaaggt ggatcatcct gttggcgctc
      301 aaactcaggc accagagctt catccggtcc cacttccttc cggagcgccc gaaccgcccc
      361 gtgaagttga taaagttgcc gagcgatctc ggcctgttgc cggatccagg ttcgctccgc
      421 atcgagggat tcggcgattt cggcgaggta gcggatccgg gccgccggga tgatggcgac
      481 gcgttgggca gtgagtcgct cgtgtgccga gacctccccc ccaaagggtg ccccggtctt
      541 gcgcgccagc acctccatga ccaggcggta gaggtcgttc atcccgggat cgttgaactg
      601 agaggcgatc gtaccgacga tcgggatctc cgcctcgggg aggtcagtac cgccgtgatt
      661 gcgacgatat tgcttccgga catcccgcag ggcatcgaga gatccgggct tgtcgaactt
      721 gttgatcgcg atcacgtccg cgaaatcgag catgtcgatc ttctcgagtt gcgtggccgc
      781 gccatagtcc gcagtcatca cgtagaggga cacgtctgcg tgatcgacga tctgggtatc
      841 ggactgcccg atccctgagg tttccacgac cacgaagtcg aagcccgccg ccttgacgat
      901 cgcgatcgcc tcggtgactt gccggctgag cgacaggttc tcgccccgcg tggcgaggga
      961 tcgcatgtag acacgcgggg taaaaatcga gttcatccga atccggtcac cgagcagtgc
     1021 gcccccactc ttgcgcttcg atggatcgac tgagaggatc gccaccgtct tctcggggaa
     1081 atcggcaaga aagcgtcgga tcaattcatc cacgaccgac gacttgccgg cgccaccggt
     1141 gcccgtgatg ccgagcacgg gtgtggtccg agaggcgacg agtggctcga gttgtcgagc
     1201 cagatcgtcg cggagctcag ggtagttctc ggcgagtgtg atcagcccgg caatggtgct
     1261 cgggtcgtgt gggtcgagtc gagccaggtg accgtcgaga cctggtggcc gggtcgggaa
     1321 gtccgctgtc ttgaggagat cgtcgatcat cccctggagt cccatcgacc gcccatcatc
     1381 cggtgagtag acccttgcga tcccggcggc gtggagtgcc tcgatctctt caggcaggat
     1441 ggtcccgcca ccacccgcaa agatcttgat gttggcgcct cgttcctcga ggagctgatg
     1501 catgtacgaa aagaactcca ggtgcccacc ctgataggag gtgaccgcga tggcctgtgc
     1561 atcttcgtgg atggcgcatt ccacgatctc ggcggcagag cgattgtgac cgaggtgaat
     1621 tacctcggca ccggagccct ggagaatacg acgcatgatg ttgatggccg cgtcgtgacc
     1681 gtcgaagagg gaggcggcag tcacgatccg gatggggtgc ttgggacggt atcgttccat
     1741 cttcacaatc taacgtggga ccagctctgc cgcctcgtgg tggggcgagg taaaccttga
     1801 gaagtcaaca tgatgcagct attctcacgc agatatttcc ccctgccagg tttgcaacga
     1861 tcatgtcccg cacagatgtc tccgctcaac tcaaacgcct ctcggacatt cgcccgggac
     1921 ctcatccgat catcagctgc tatttgaagc tcgagccaag agaccggtct cgcgggaagt
     1981 acctgatcaa gctcaagaat cgggttcggc aggtgcttgc agaactcccc gccatgggat
     2041 tggaccg
//
LOCUS       DATDOZ010000088         4087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3742524, whole genome shotgun sequence.
ACCESSION   DATDOZ010000088 DATDOZ010000000
VERSION     DATDOZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3742524"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..352)
                     /locus_tag="VI541_01685"
     CDS             complement(8..352)
                     /locus_tag="VI541_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HLF69646.1"
                     /translation="MKIEGDGRLLRIFIGESDTWHGKPLYEAIVEKLRAEGLAGATAI
                     RGVEGFGAHSRLHTSRILRLSQDLPIVIEVVDKPERIDTIIPMLDEMVSEGMLTVEDV
                     HIIAYRSGGSEL"
     gene            complement(349..699)
                     /gene="crcB"
                     /locus_tag="VI541_01690"
     CDS             complement(349..699)
                     /gene="crcB"
                     /locus_tag="VI541_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HLF69647.1"
                     /translation="MGALGALSRYGLEGLVSKRFPGAFPWGTFAVNITGAFLLGLIFT
                     VTTERLTISPPIRSGITIGFLGAYTTFSTLSLESFRLIEQGAVGLGLLNSFASLGAGL
                     VATFLGVTTGRAIG"
     gene            807..2294
                     /locus_tag="VI541_01695"
     CDS             807..2294
                     /locus_tag="VI541_01695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF69648.1"
                     /translation="MRTPKSVWILASLVFAASLILASSFGPHRDELYFIACARRLAWG
                     YVDQPPLVPAIYRLGLLMGQPVLLPRWIAAAMAASLPLVIFRMTIELDGNRNAGIISS
                     TAIAVTPFLFGSSYFMGPTTVDIFVWSVATLIFIKILHTDDHRLWLRLGGVVGLGLLN
                     KHTILYFVASMGAGLLIAGRAHLRTWWPWAGLATAIVIWAPNLVWQSSNGWPTLEMIG
                     SLRRVNGGLVSGILFLPSLFVISGPVTSILAIGGAAWLLRSKRAAYYRSFGWMCLATM
                     IAVPLSGGRPYYAAPIVIPALGAGARWFDEHQWRVRRVVPLIVIAGVLPLIPLANSLI
                     SAEVEESRSWAPMAAAVEDVRHALPEAERDVAIVLASDRGQAAAIERFAKGSPVFSGH
                     NNFWEWGPPPGVIGVTVAVGFRHPNNQLQPYLGDCSLTDVFRSKHRDTPIYLCRGSGD
                     WRRIWPRFRHYDTSRWVSAKGLCSVAEVLERSLGRGGTFCFGTKG"
     gene            2308..2829
                     /locus_tag="VI541_01700"
     CDS             2308..2829
                     /locus_tag="VI541_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HLF69649.1"
                     /translation="MSEQVTKQVAVETLAEGHRLLFEAIDPLSGDLIDKHGLGGGEWS
                     IKDLIGHLESWERAALDTIEDRRAKRSPRIRQLVHDAESMNSFNASEVDRKRTRRWEE
                     QRAAYQVTHDLLIETIAEMDDSEWSNPDDEGWTLGMQVGSNTGAPERPFGHVFAHIED
                     VRNVVHLARGGSG"
     gene            2840..>4087
                     /locus_tag="VI541_01705"
     CDS             2840..>4087
                     /locus_tag="VI541_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLF69650.1"
                     /translation="MNEIRLAGLLVPLSLVSDLGMGFAPEHSIRNCLLAMVVGREMGL
                     AVEDLSDLYYTALLFDIGCTASAHEFAMFVGGDDVAMRGLSAVTDFADAKQSLAFMKQ
                     VGSGLRPGARTLTRMKAMASGSRAGRYFVRGTCEVGTRLVARLGLGPGTVRAIDQIFE
                     RWDGKGDPHGLEGESIALVARIVSATYLALSVHEVAGAEAAIAELRRCAGSSLDPQVV
                     EVVAKGCNKMFAHFDETDPFAEAIDAEPRPIKIFPSTHLREVALAFTDLADLKTPHTA
                     GHSRAVADLVTAAGSRGPGSDLELAALLRDLGRVGISNSIWSKPGPLTISEWESVRLH
                     AYHSERIISKVPALSALAPLVGMHHERLDGSGYHRGSRGSEISLDVCLIAAADAYHSM
                     IQDRPYRGAMTLGQAALALQEEVT"
BASE COUNT          757 a         1209 c         1270 g          851 t
ORIGIN      
        1 aggatcgcta gagttccgat ccccccgatc gataggcgat gatgtggaca tcctcgacgg
       61 tcaacatccc ctcgctcacc atctcatcga gcatcgggat gatggtgtcg atccgctccg
      121 gcttgtcgac gacttcgatg acgatgggaa ggtcctggct caatcgcaag atgcgtgacg
      181 tgtggagtcg actgtgggcg ccgaacccct cgacccctcg gatcgcggtt gccccggcca
      241 acccctcggc cctgagcttc tctactatgg cctcgtaaag aggctttcca tgccaggtgt
      301 ccgattcacc gatgaagatg cgaagcaacc tgccatctcc ttcgatcttc atccgatcgc
      361 cctccccgtt gtcactccga gaaaggtggc tactagtccg gcccccagac tcgcgaagct
      421 attcaacaga cccagcccca cggcgccctg ttcgatgagt cgaaaggatt caaggctcaa
      481 cgtcgagaat gtggtgtagg caccgaggaa cccgatggtt atcccggatc ggatgggcgg
      541 tgagatggtc aggcgctccg tggtgactgt gaagatcaga cccaagagga acgcacctgt
      601 gatgttgact gcgaacgttc cccaggggaa cgcgccgggg aatcgtttac tcacgagacc
      661 ctcgagaccg tatcgcgaga gtgctcccag ggcgcccatc gctccgatga ggccgatgtt
      721 tcgccagctc aatgatccaa ggcgcaggtc ggatgaagag tcatggagac aaatgtatac
      781 ctaccctgcg ggataatcgg ccggttgtgc ggaccccaaa gtcagtctgg atccttgcgt
      841 cgctcgtgtt cgccgcgagt ctcatccttg cttcgagctt cggcccccat cgtgacgagc
      901 tctacttcat cgcttgcgcg agacgcctgg catgggggta tgtagaccag ccccccctcg
      961 tgcccgcgat ctatcgacta gggctactga tgggacaacc cgttctttta cctcgttgga
     1021 tagccgctgc gatggccgcc tcgctgcccc tagtgatctt ccgcatgacc atagaactgg
     1081 atgggaaccg gaacgcgggc atcatctcgt caactgccat cgcggtcacg ccgttcctgt
     1141 tcgggtcttc ctacttcatg gggcctacca cggtcgatat cttcgtctgg tcagtcgcga
     1201 ccctcatctt catcaagata ttgcacactg atgatcatcg gctatggctc cggctcggag
     1261 gagtggtcgg tcttggcctg cttaacaagc acacgatcct ctacttcgtg gcgagcatgg
     1321 gtgccggtct tctgatcgcg ggccgcgcac accttcgaac ctggtggcct tgggcagggc
     1381 tggcaaccgc gatcgtcatc tgggccccca acctcgtttg gcagtcgagc aacgggtggc
     1441 caacgctcga gatgatcgga tcgttgaggc gggtgaacgg tggactcgtc tcagggatcc
     1501 tcttcctgcc tagtctcttc gtgatcagcg gtcccgtcac ttcgatacta gccataggag
     1561 gtgctgcctg gctgcttcgt tccaagcgcg cagcgtatta ccgctccttc ggatggatgt
     1621 gtctcgcgac catgatcgcg gttcccctca gcggcggacg tccctactac gcggctccga
     1681 tagtcatccc tgcgctcggg gcgggagccc gttggttcga cgagcaccag tggagagttc
     1741 gccgggtagt gcccctcatc gtgatcgctg gtgtcctgcc cctgatcccg ctggccaact
     1801 cactcatctc agccgaggtc gaggagtccc gatcgtgggc accgatggcc gcggccgtgg
     1861 aagatgtgag gcacgccctc ccggaggcag agcgcgatgt tgcgatcgtg ctcgcgagcg
     1921 atcgcggtca agccgctgcg atcgagaggt tcgcgaaggg gtcaccggtg ttcagcggac
     1981 ataacaactt ctgggagtgg gggcccccgc cgggtgtcat cggagtcacg gttgcagttg
     2041 ggttccgaca cccgaacaac cagctacagc cttaccttgg ggattgctcg ctcaccgatg
     2101 tgttccgctc gaaacatcgc gacactccca tctacctctg ccgaggctct ggagattgga
     2161 gaaggatatg gccgcggttc cgtcactacg atacgtctcg atgggtgagt gctaagggcc
     2221 tatgctccgt ggccgaggtc ttggagcgat cgctcggccg tgggggaacc ttctgttttg
     2281 gaacgaaagg gtgaggagga tcgaatcgtg agtgaacagg tgaccaaaca agtagctgtc
     2341 gagaccctag ccgaagggca tcgtctgctc ttcgaagcta tcgatccact ttccggcgac
     2401 cttatcgaca agcatggcct tggtggaggc gagtggtcga tcaaagatct gatcggacac
     2461 ctcgaatcgt gggaacgagc ggccctcgac accatcgagg atcggcgagc caaaagatcc
     2521 cctcgcatcc gtcagttggt ccatgatgcg gaatccatga atagtttcaa tgcaagtgag
     2581 gtcgatcgga aacgtactcg tcgttgggaa gagcagagag ccgcgtatca agttactcac
     2641 gacctgttga tcgagacgat cgcagagatg gacgacagcg agtggtccaa tccggatgac
     2701 gaggggtgga cgctcgggat gcaggtcggc tcaaacaccg gggcacccga acgtcccttc
     2761 ggtcacgttt tcgcccatat cgaggacgtc cgtaacgtcg tacatctagc gagaggcggc
     2821 tcggggtagg gtctcggagg tgaacgagat aaggctcgca gggttgctgg ttcccttatc
     2881 gctggtaagt gaccttggaa tggggttcgc cccggagcac tcgatccgta actgtctgct
     2941 ggcgatggta gtgggtcgag agatgggtct ggctgtcgaa gatctgagcg acctctacta
     3001 caccgccctc ctcttcgaca tcggatgtac ggcgtcagcc cacgagttcg cgatgttcgt
     3061 cggtggtgac gacgtagcga tgcgggggct ttccgcggtc acagatttcg ccgacgcgaa
     3121 acagagcctt gcgttcatga agcaggtcgg atcggggctc cgaccggggg cccggaccct
     3181 cacccgtatg aaggcgatgg cgagcggtag ccgggctggt cgatacttcg ttaggggcac
     3241 gtgcgaggtc ggcactcgac tcgtcgccag actcggcctc ggtccgggga cggtccgtgc
     3301 catcgaccag atattcgaac ggtgggatgg gaagggggat cctcacggcc tcgaaggcga
     3361 gagcatcgct ctggttgcgc ggatcgtctc ggcgacatat ctcgctctgt ctgtccatga
     3421 ggtggcggga gccgaggcgg cgatagccga gcttcgtcgt tgcgccggtt cgagcctcga
     3481 tccgcaggtc gtcgaagttg tggcgaaggg ctgcaacaag atgttcgctc acttcgatga
     3541 gaccgacccg ttcgcggagg cgatcgatgc agaacctcga cccatcaaga tcttcccgtc
     3601 gacccacctt agagaggtag cacttgcttt caccgacctg gccgacctga agactccgca
     3661 cactgcgggg cactcacgag ctgtggctga cctggtcact gccgctggta gtcgggggcc
     3721 tgggtccgat ctcgagctgg cggcgctgct tcgcgatctg ggccgggtgg ggatctcgaa
     3781 ctccatctgg agtaagccgg gcccgctgac tatctcggag tgggagtccg tgaggctgca
     3841 tgcctaccac tccgagcgga tcatctcgaa ggtgccagcc ctatccgccc tcgctcctct
     3901 cgtcggcatg catcatgagc ggctcgatgg gtcggggtat caccgcggct ctaggggatc
     3961 agagatctcg cttgatgtgt gcttgatagc tgcggccgac gcctatcact caatgatcca
     4021 ggatcgtccg tataggggtg ccatgacact cggccaagcc gccctcgcac tacaagagga
     4081 ggttacc
//
LOCUS       DATDOZ010000089         3205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3743559, whole genome shotgun sequence.
ACCESSION   DATDOZ010000089 DATDOZ010000000
VERSION     DATDOZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743559"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VI541_01710"
     CDS             complement(<1..443)
                     /locus_tag="VI541_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HLF69651.1"
                     /translation="MKDLTWPHILGPLLAGQSLDAAHSAWAMRSIMEGEATPAQFGAF
                     VAGLRAKGETVDEIFGLVTTMREMSQKVVVEGPLLDTCGTGGDRSGTINVSTIAALVV
                     AGAGVRVAKHGNRAASSACGSADLLEELGVKIDLGPQGVAACIEGA"
     gene            complement(475..1851)
                     /locus_tag="VI541_01715"
     CDS             complement(475..1851)
                     /locus_tag="VI541_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF69652.1"
                     /translation="MGAGRPSCRTRRGVRPDHGEDASWPRPDASGAPRESVGLTDSPG
                     PPERRAWRSLAIDLSPLRTSRDFRLLWIGSLVSETGRNITMVAVFSQVYKITGSAAAV
                     GFVGLMQFAGLFFSSIVGGSIVDAVDRRKMLVVTQFGLAATALALVIGATLPRTPLLL
                     IYVAVTVASALGGLAGPTRSAMLPRLISKDQMPTAIALTQVMWNTTMVVGPAVGGVVI
                     AKLGFAWAYGIDLSTYVASITAALMLRPIKPGDDESAPATGIAAIREGLRFLRGHRLI
                     QSTFGIDLIAMVFGMPRALFPVLAVVQFHGGSEIAGLLFAAPALGALIGAASAGWVDR
                     VKRQGLAVIVSVGIWGAGITAFGFSGNHLIPALTFLAIAGAADVISAVFRSTMLQLNL
                     PDALRGRLSAINLLVVAGGPRLGDAEAGLVANAFTPTISVVSGGVACIVGAVAFGAMV
                     PAFRRYRA"
     gene            complement(1734..2237)
                     /locus_tag="VI541_01720"
     CDS             complement(1734..2237)
                     /locus_tag="VI541_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF69653.1"
                     /translation="MSLDEATEYRNALLAALGDDDPLEVLGSTVESVRALTEGVPGER
                     MIAEPAPGEWSARHVLSHWYDTDLVYGIRIRMTLTQDRPVLVGYDQGAWAGRFGLLDD
                     NARQTHLRWRWVRENNLRLYRSLSDEEWERVGLHAERGEESVRTMVRMLAGHDRMHLE
                     HLASLLA"
     gene            complement(2249..>3205)
                     /gene="ligD"
                     /locus_tag="VI541_01725"
     CDS             complement(2249..>3205)
                     /gene="ligD"
                     /locus_tag="VI541_01725"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HLF69654.1"
                     /translation="EVAGLVSAHAIPLEKRKDAHFFHESDREVRITNIDKVLFPDDGL
                     TKGDLISHYYNVAPLMLPFLRDRPINMQRVPDGIYGEAFYEKQCPKGAPEWVRTVPVP
                     SDGGRRDIDYIIVEHASTLVWMAQIASVECHAWTSRWPNLDEPDFAVMDLDPHEPITF
                     DDVRAVASMVKVLLEKLGLRGFPKTSGGSGIQIFIPLIEGHTYPEVREFCSLVGGLIR
                     AAYPEKVTMEPSKAARVAKVYIDANQNAKSKTLVAPYSVRPYPGATVSTPLSWDEIAE
                     PFMPEEFTITTILQRIASVGDLFRPALSSRQDLHAALDQLRG"
BASE COUNT          643 a         1007 c         1010 g          545 t
ORIGIN      
        1 gccccctcga tgcacgcggc cactccttgg gggcccaggt cgatcttgac tcccaactct
       61 tcgagcaggt cggccgagcc gcaggcgctc gacgccgcgc ggttgccgtg cttggcgacc
      121 ctcaccccgg cccccgcaac gacgagagca gcgatcgtcg agacgttgat cgtgccggaa
      181 cggtcgccgc cggtgccgca tgtatcgagg agtggaccct cgacgacgac cttctgtgac
      241 atctcgcgca tggtcgtgac aagaccgaag atctcatcga cggtctcacc cttcgcacga
      301 agacccgcga cgaatgcacc gaactgtgct ggggtcgcct cgccctccat gatgcttcgc
      361 atggcccagg ccgagtgcgc ggcgtcgagc gattgccccg cgaggagggg gccgaggata
      421 tggggccagg tgaggtcttt catcgtgaga ttctcgcacc tgcgagacgg aacctcatgc
      481 gcgataccgt cggaaggccg gcaccatggc accgaaggcc acggctccca ctatgcaggc
      541 cactccaccc gacacaaccg agatcgtagg agtgaatgcg ttcgccacga gtccggcctc
      601 ggcatcaccg agacgcggcc cgccggctac cacgaggagg ttgatagccg agagacggcc
      661 tcgcagcgcg tcaggaaggt tgagttgcag catcgtcgag cggaacaccg ccgagatcac
      721 atcggccgcg cccgctatcg cgaggaaggt cagtgcaggg atcagatggt tgcccgagaa
      781 gccgaacgcc gtgatgccag caccccagat gccaaccgaa acgatcacgg caagaccctg
      841 ccgcttcacg cgatcgaccc acccggcact cgccgcaccg atgagggccc cgagcgctgg
      901 ggcggcgaag agcagaccgg cgatctcgga cccaccgtgg aactggacga ccgcgagaac
      961 ggggaagagg gcccttggca tgccgaagac catcgcgatc aggtcgatcc cgaatgtgga
     1021 ctggatcaag cggtgccctc gcaggaaccg caggccctca cggatcgctg cgatgcccgt
     1081 cgccggagcg gattcatcat ctcccggctt gatgggccgc agcatgagag cggcggtgat
     1141 cgaagcgacg tacgtggaga ggtcgatgcc gtaggcccat gcgaacccca gcttcgcgat
     1201 gacgacaccg ccaacggctg gcccaacgac catggtcgtg ttccacatca cctgcgtcag
     1261 agcgatcgcc gtcggcatct gatctttgct gatcaggcga ggcagcatgg cagagcgggt
     1321 tggccccgcg aggcccccga gcgccgatgc gactgtgact gcgacataga tgagcaggag
     1381 cggtgttcgc ggaagcgttg caccgatgac aagggccaag gccgtcgcag cgaggccgaa
     1441 ctgcgtgacg accaacatct tgcgacggtc cacagcatca acgatggaac cgccaacgat
     1501 agatgagaag aagagccccg cgaactgcat gaggccgacg aacccaaccg cggcggccga
     1561 cccggtgatc ttgtagacct gcgagaagac cgcgaccatc gtgatgttac gacctgtctc
     1621 ggatacgaga cttcctatcc agagcagacg gaagtcgcgc gacgtgcgaa gaggcgagag
     1681 atcgatggcg agagaacgcc aggcccggcg ttcaggaggg cccggactat cggtcaagcc
     1741 aacagactcg cgaggtgctc cagatgcatc cggtcgtggc cagctagcat cctcaccatg
     1801 gtccggacgg actcctcgcc gcgttcggca tgaaggccga cccgctccca ctcctcgtcg
     1861 ctcaacgatc ggtacaggcg cagattgttc tcgcgcaccc accgccaacg aagatgtgtc
     1921 tgtcgagcgt tgtcatctag gaggccgaat cgccctgccc aggccccctg gtcgtagccg
     1981 acgagcacgg gtcgatcctg cgtgagcgtc atccggatac ggatgccata gacaagatcg
     2041 gtgtcgtacc aatgcgagag gacatgacga gccgaccact cgccgggtgc gggttcggcg
     2101 atcatccttt cgccagggac cccttcagtg agtgccctga ccgactcaac ggtcgacccg
     2161 agaacttcga gcggatcatc gtcaccgagt gccgcaagca atgcgtttcg atactccgtg
     2221 gcttcatcga gactcaactt gtgtcctcct atcctcttag ttgatcgaga gctgcgtgta
     2281 gatcctggcg cgacgacagc gcggggcgga agaggtctcc aacgcttgcg atacgttgaa
     2341 ggatcgtagt gatggtgaac tcctccggca tgaacggctc tgcgatctca tcccacgata
     2401 gtggagtcga gaccgtcgcg ccggggtacg ggcggaccga gtacggcgcg accagggtct
     2461 tgctcttggc gttctggttc gcatcgatat agaccttggc tacgcgagcc gctttggacg
     2521 gctccatcgt gaccttctcc gggtaggccg ccctgatgag gcccccgacg agcgagcaga
     2581 actctctgac ttcaggatag gtatggcctt cgatgagagg gatgaagatc tggatgccgg
     2641 aaccgcccga agtcttcggg aatcctctga gccccaactt ttcgagaagg actttgacca
     2701 tcgatgcgac cgctctcacg tcgtcgaagg tgatcggttc atggggatca agatccatca
     2761 cggcgaagtc aggttcatcg aggttcggcc agcggctcgt ccacgcgtga cactcgacac
     2821 tcgcgatctg cgccatccag acaagcgtcg acgcatgttc cacgatgatg tagtcgatgt
     2881 cgcgccgccc gccatcggag gggactggaa ctgttcgcac ccactcgggg gcccccttcg
     2941 ggcactgctt ctcgtagaag gcctcgccat agattccatc gggaacgcgc tgcatgttga
     3001 tgggccgatc tcgcaggaac ggcagcatca acggagcgac gttgtagtag tgcgagatga
     3061 ggtcaccttt cgtgaggcca tcgtcgggga agaggacctt gtcgatgttg gtgatgcgca
     3121 cctcgcgatc tgactcgtgg aagaagtgcg catcttttcg tttctccagt ggtatcgcat
     3181 gggccgacac gagcccggcg acctc
//
LOCUS       DATDOZ010000090         8670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1897869, whole genome shotgun sequence.
ACCESSION   DATDOZ010000090 DATDOZ010000000
VERSION     DATDOZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897869"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /gene="lnt"
                     /locus_tag="VI541_01730"
     CDS             <1..1798
                     /gene="lnt"
                     /locus_tag="VI541_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HLF69655.1"
                     /translation="SLLVLVVNVSIASAIGGWRKRGPRALGYLVVVGLVMALPVLTSF
                     RDADGTPVKVAMIQGNAPEGTENPHFDDAKVIRDHARLTDGLETRPDLVIWPESAFDL
                     DPLRDPVMAEPLYRSIGLVQTPFLVGAFREEFATSGPTFLGNPQFRMHTYYNASLFFG
                     PDGSLLGEYHKRHLVPFGERVPLRPFFSRFVKAIERVPNDLTPGSEATVFDLPQGRFA
                     SVICFESTFPELVRSSVVKGARLLVVSTNNSSFERTAASAQHVAFSQLRAAEHRVWIA
                     HAALTGISAVVAPDGRVLERTGLFEPAVLTPTMRMATGITPYGRWGDWLPISTLIILG
                     ILAVAALLRRPWRTRPPQPFDREPTLVMIPTYQEAMNIEAVMTAVMEADRSLHILVVD
                     DDSGDGTADLASAAGERWPGRCNVLVRPGKTGLGRAYVDGMRWGLEHGYATLVEMDAD
                     LSHDPGALPGLLEKVETYDVVIGSRYIKGGEVTGWSRARHTLSRAGNIYVETLLGMGV
                     KDATSGFRAYRREVLEAIDLDSVRSDGYAFQIDMTYRARRRGFTITEYPITFSERRAG
                     ESKMSRSIIIEAVIAVARWALRDTFTRRGPSL"
     gene            complement(1842..2582)
                     /locus_tag="VI541_01735"
     CDS             complement(1842..2582)
                     /locus_tag="VI541_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69656.1"
                     /translation="MIPNSAMPDLASLLRRVAVGVVLGLAAMTANLAEATPAATTYHY
                     AKPSTNIVRDVTGQGSASAAATVSGSSTRIFNSNGSFSVSASATSTVPYVTNVSDKGF
                     DVGVGVGYNSGYAQVFFRHTFEVAPTTSTAYSVATALNLSSASGNYSADPPTMGQIYS
                     ASRYTAQAYMTVTFYRPDGTFTTGSGQASDIPLGTNTSLTLNGSVNAGQVGNVVVDVY
                     VACFAWAQGTASSNVQAAGALTSITLTP"
     gene            complement(2579..3238)
                     /locus_tag="VI541_01740"
     CDS             complement(2579..3238)
                     /locus_tag="VI541_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69657.1"
                     /translation="MTSNLAGAAQSAPPYAYGSPVKSFVRDVTMQGSANAVATVDGSS
                     VPVLNSNGSFSVSGSATSTVPYVTNVSDKGLGYNSAYAKAYFYHEFPVAPGLYSVTST
                     LNFTSASGSNTSTSTTLGTFYPFYPGYTIYSASGYTAKAYLQLRFRPSWGGSPTPAYG
                     PSENIPLGSNTSRTLSSSITAGEAGYIIVEVYIECSAYAQGTAAANAQAAGTVTTTLS
                     P"
     gene            complement(3301..3990)
                     /locus_tag="VI541_01745"
     CDS             complement(3301..3990)
                     /locus_tag="VI541_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69658.1"
                     /translation="MRAHVLRRIALGALLGMAAMTANLAGATQSAPPYQYGSPSKSFV
                     RDVTMQGSANAVATVDGSSAPVLNSNGGFSVSASATSAVPYVTNVSDKGLGYNSAYAQ
                     VFFHHEFTVTTPTLYSVTSTLNFTGASGTYSAAPPIQATIYSAGGYGSQLYLKVYFYP
                     SSGPSTQGSGQNDYIPLGTNTSKTLVGSVTASSAGKIVIEVLVQCNAYAQGTASASIQ
                     AAGTLTTTLAP"
     gene            4155..5207
                     /locus_tag="VI541_01750"
     CDS             4155..5207
                     /locus_tag="VI541_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLF69659.1"
                     /translation="MKRESTLGAMHSWLMEMGNDGRSTRTLDAYDSDVTDTVEVIAGL
                     LGTKPEQVTLDQIDRDLLVAAIADFRTRPDPRYTVNPDQAPTQRSPARVARRVAAVKV
                     FFKWCYATGRIASDPAALLRSPKRPKRLPKALELTAAADLMGLPGVSRWPERDQLIVV
                     LALTTGLRLEEMSTLKMADISGDPPASLTVIGKGNKERGLPLPAITSHAIEAYIPTRR
                     KRLEKLGLDSKTLFISTRPRRVGARKDGSAIISVDATRAGIAYVVDRMLSRIGARRRG
                     SRVHVLRHTFATLGLRPDPNTGQPAYTLRQLQSALGHANLSTIQVYAEVSDAELVQAS
                     AAHPLSHVKVGSGADQ"
     gene            complement(5190..5681)
                     /locus_tag="VI541_01755"
     CDS             complement(5190..5681)
                     /locus_tag="VI541_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69660.1"
                     /translation="MSSLLRARFALILLLATFLIPIGVSGLRGLTHVLSCSSSVATPF
                     TITILPDGVPTITSSRTLTRDDDSSLCAIPDPSTGIEEGLVVDLAARGTSGKIEMIVI
                     ITNQTHRPWQGTVQLNLGGQRRLSYPVTIGRIPARQVRRATVLLELPKGLTDIDGKLL
                     IGP"
     gene            complement(5685..7130)
                     /gene="gcvPB"
                     /locus_tag="VI541_01760"
     CDS             complement(5685..7130)
                     /gene="gcvPB"
                     /locus_tag="VI541_01760"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HLF69661.1"
                     /translation="MSTIFDLSSKGRRASSIPASGVEASAHIGSLPSKFRRQQAPNLP
                     EVSELDLVRHYTNLSRMNWAIDVGPYPLGSCTMKYNPKVHEQMAALPGFRTLHPWQSA
                     EQTQGALQILWEMERALAEITGMARVTFQPAAGAAGELTGLLLMRAFHAAQGSPRTHV
                     LIPDSAHGTNPATVRLAGYQVRQIPSDENGLVDLTALEEAADENVAGLMLTNPNTLGL
                     FERDIEKIAAIIHRVGGLLYYDGANLNAIMGIIRPGDMGFDVVHMNLHKTFTTPHGGG
                     GPGSGPVAVSQRLERFLPGPLVAYDPQTSRYRWDADRPDSIGHIHGFHGNFAMVLRAY
                     TYIRSLGSVGLHRVSERAVLNANYLRARIESVYPMAHPGLCMHEFVATAKEFRKQGLR
                     GMDVAKRLIDLGFHPPTVYFPLVVEEALMAEPTETESKETLDALADALLQIADEAESS
                     PELLTDAPTTTPVGRLDEARAARQLHLRWQP"
     gene            complement(7127..8449)
                     /gene="gcvPA"
                     /locus_tag="VI541_01765"
     CDS             complement(7127..8449)
                     /gene="gcvPA"
                     /locus_tag="VI541_01765"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HLF69662.1"
                     /translation="MDYTPHTDGDVASMLDTIGVTSIEDLFSQIPSSLRLGRELDLPD
                     QLTESEVTDLVTQIAAKNIPTDSLRCFAGGGAYDHYIPAVVRSVAYRSEFVTSYTPYQ
                     PELSQGVLGALFEYQTMICELSGMEVANASLYDGAAALVEAVNLAVGATGRNKIVVTE
                     ALNPNYRKVLETFGAGIGLNFVTAPAPRGRTEPAAIDDAACLILAQPNFFGCIEDVGA
                     VATQVHAAGALMVVHMDPLAMGTLEAPGKLGADVVTGEGQSLGNDLNFGGPYLGIFAT
                     TMEHVRRVPGRICGQTVDVAGKLGFVLTLQAREQHIRREKANSNVCTNQTLMAIVATV
                     YLAWLGPQGLVELGETCSARAAHAAELLASVPGCSLVFEAPFFKEFVLRLPRPAEQVL
                     SDLSERGYLAGPAVVLADGSEGIIVAVTERRTLEQIEGFAKALAEVVA"
     gene            complement(8450..>8670)
                     /locus_tag="VI541_01770"
     CDS             complement(8450..>8670)
                     /locus_tag="VI541_01770"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HLF69663.1"
                     /translation="KGREIPRQGYEVFRDDEKVGDVTSGNFSPVIRCGIAMAYVSTEV
                     AAPGTMLQVDVRGRRADMEVTRPPFIRR"
BASE COUNT         1677 a         2557 c         2737 g         1699 t
ORIGIN      
        1 aagcctgctt gtcctggtcg tgaacgtcag catcgcatcg gcgatagggg gctggcgcaa
       61 gcgggggccc cgtgcactcg gctacctcgt ggtggttggg ttggtgatgg cgcttccagt
      121 gctcacctca ttccgcgatg cggacggaac gccggtcaag gtagcgatga tccagggcaa
      181 tgctccggaa ggcacagaga acccccactt cgacgacgct aaggtcatca gggatcacgc
      241 gcgcctcacc gatggccttg agacacggcc cgacctggtc atctggcctg agagcgcgtt
      301 cgacctcgac ccattgcggg atccggtcat ggccgagccc ttatatcgat ctatcggcct
      361 cgtccagact ccgttcttgg tgggagcctt ccgcgaggaa ttcgctacca gcggacctac
      421 ttttctcggg aatcctcaat ttcgaatgca cacctactac aacgctagcc tcttcttcgg
      481 tccggacggg tcgttgttgg gggagtacca caagagacac ctcgtcccct tcggcgagag
      541 ggtgccactc cgtccgttct tctctcgatt cgtcaaggcc atcgagaggg tccccaacga
      601 cctcacaccc ggcagtgagg cgacggtgtt cgatctgccg cagggccgct tcgcatcggt
      661 catctgtttc gagtcgacct tccccgaact cgtaagaagc tccgtagtca agggagctcg
      721 gcttctcgtt gtctcaacga acaactcgtc gttcgagcga acggccgcga gcgcgcagca
      781 tgttgcattc agtcagttaa gggccgcgga gcatcgcgta tggatcgcgc atgcggccct
      841 cacggggatc tctgcggtgg tcgcgcctga tggtcgagtc cttgagcgaa caggattgtt
      901 cgagcctgca gtgctgaccc caaccatgcg gatggcaacc gggataacgc cttatggccg
      961 atggggagat tggcttccga tcagcacgct gatcatcctc ggcatcttgg cggtggcggc
     1021 tctgctccgt aggccttggc gcacaaggcc cccacaaccg ttcgacaggg aaccaacgtt
     1081 ggtcatgatc cctacctatc aagaggcgat gaacatagag gcggtcatga ccgccgtgat
     1141 ggaagcagat cggtcgctgc acatcctggt cgtcgacgac gactccggcg atggcacggc
     1201 agatctggcg agcgccgcag gggagcggtg gccaggtcgc tgcaacgtgt tggtgcgccc
     1261 cgggaagacg ggtctcgggc gggcctatgt cgatgggatg cgatggggcc tggaacatgg
     1321 ttatgcaacc ctcgtcgaga tggatgcgga tctatcgcat gaccccggtg cccttcccgg
     1381 tctgctcgag aaggtggaga cctacgacgt cgtcatcggt tcccgctaca tcaagggagg
     1441 cgaggtcacg ggctggtctc gagcacgtca cactctttcg agggctggca acatctatgt
     1501 cgagactctc ttgggcatgg gggtaaagga tgctacgagc ggattccggg cttaccgccg
     1561 agaggttctc gaagccatcg acctcgacag tgtccgctca gacggctatg cgttccagat
     1621 cgacatgacc tacagagccc gccgcagggg cttcaccatc accgagtacc ccatcacctt
     1681 ctctgagcga cgggctggag agtcgaagat gtcgagatcg atcattatcg aagccgtgat
     1741 cgccgtcgct cggtgggccc tgcgagacac cttcaccaga aggggaccct ccctataact
     1801 cattacagaa caatcattta gtaaggagtg ctccctggga actacggtgt gagggtgatg
     1861 ctcgtaagtg ctccggcagc ctgaacgttt gacgaggcgg tcccctgagc ccacgcaaag
     1921 cacgcaacgt agacgtcgac aacgacgttc cccacctgac cggcgtttac ggagccgttc
     1981 agggtcagag aggtattcgt ccccaagggt atgtctgagg cttggccgga gccagtggtg
     2041 aacgttccgt caggtctata gaaggtgacc gtcatgtatg cctgggcggt gtagcggctt
     2101 gccgagtaga tctgacccat agttgggggg tccgcagagt agttgcctga agcgctcgac
     2161 aggttcagtg ccgttgccac ggaatacgcc gtactcgtcg tcggagcgac ctcgaacgtg
     2221 tgcctgaaga agacctgcgc ataaccggag ttgtagccga cacctacacc aacatcgaaa
     2281 cccttgtcac tcacgttcgt tacgtacggg actgtactgg tcgccgatgc tgaaaccgag
     2341 aaggaaccgt tggagttgaa gatcctagtg ctgctgccgc tgaccgtggc tgcggcgctc
     2401 gcggacccct gccccgttac gtcccggacg atattggtgg agggcttcgc gtagtgatac
     2461 gtggtggcag cgggtgttgc ctcggccagg ttcgccgtca tcgccgctag ccccagcact
     2521 acaccgacag cgacgcgcct tagaagggat gccaggtctg gcatcgcgga gtttggaatc
     2581 atgggctgag tgtggtcgta acggttccgg cggcctgggc gtttgcggcg gctgtgccct
     2641 gagcatacgc gctgcactcg atgtaaacct cgacgatgat gtatcccgcc tctccggcgg
     2701 tgatagaact gctcagagtc cgagacgtat tcgaccctaa ggggatgttc tcacttggcc
     2761 cgtaggctgg ggttggcgaa ccgccccaac tcggtcggaa tctgagttgg aggtacgctt
     2821 ttgccgtgta gccgcttgcc gagtagatgg tatagcccgg gtagaacggg tagaacgtac
     2881 ccagagtcgt gctggtcgac gtgttggatc ctgaggcgct tgtgaagttc agcgtgctgg
     2941 ttaccgaata gagcccagga gcaaccggga actcgtggta aaagtaggcc ttcgcgtaag
     3001 ctgagttgta accaaggcct ttgtcactta cgttcgtcac gtacgggacg gtgctagtcg
     3061 ccgatcctga gacggagaaa gaaccgttgg agttaaggac cggcacgcta ctgccgtcga
     3121 cggtcgctac ggcgttggcg gacccctgca tcgttacatc tcgtacaaaa cttttgacgg
     3181 ggcttccgta cgcataaggg ggggccgact gcgctgcccc cgccaggttg gatgtcatcg
     3241 aggccatccc cagaaagacc cccagggcga cgcgtctcag acgctgtgtc gggccgttga
     3301 tcacggagct agtgtggtcg taagtgttcc ggcggcttgg atgcttgccg aagctgtgcc
     3361 ctgtgcatac gcgttgcatt ggacgaggac ctcgataaca atcttcccgg ctgagctggc
     3421 ggtgaccgag ccgaccagcg tcttggaggt attcgttccg agcggtatgt aatcattttg
     3481 cccggagccc tgggtagacg gcccggaact cggatagaag taaaccttca agtacagttg
     3541 cgatccgtaa ccgcctgctg aatagatcgt agcttggatc gggggggctg ccgagtacgt
     3601 tcctgaggcg cccgtgaagt tcagagtgct cgttaccgag tagagcgtag gcgtcgtaac
     3661 cgtgaactcg tggtggaaga agacctgagc gtaagccgag ttgtaaccaa ggcccttgtc
     3721 actgacgttc gtcacgtacg ggacggcgct ggtcgccgat gcagagaccg aaaagccacc
     3781 gttggagtta aggaccgggg cgctgctacc gtcaaccgtc gctactgcgt tggcggaccc
     3841 ctgcatcgtc acatctcgca cgaagctttt cgaggggctt ccgtactgat aggggggggc
     3901 cgactgcgtt gcccccgcca ggttggctgt catcgccgcc atccccagca gcgcccccag
     3961 ggcgatgcgt cttagaacgt gtgctctcat cgtctctccc ccgtcgaagg acccgacttc
     4021 ggtcagcgcc gactatcccg tcgagagagt ggttcgacgg gatatcggga cattccttcc
     4081 tgccaaggcg gaaaactcac atccgaaagc gcaaatacac gcctcagccg acacctgagc
     4141 gcagtattgg gctcgtgaag agagagtcta ccctcggtgc gatgcattcc tggctcatgg
     4201 agatgggcaa cgacgggcgg tccacacgga cgctcgacgc ctacgacagc gacgtcaccg
     4261 acaccgttga agtgatcgcg ggactcctgg gaacgaaacc tgagcaggtc accctggacc
     4321 agatagatcg cgacctgctc gtggccgcca tcgccgactt tcgaacgcgg ccggacccga
     4381 ggtacacggt caaccccgac caggccccca cgcagcgaag ccctgccagg gttgcccgaa
     4441 gggtcgccgc cgtcaaggtg ttcttcaagt ggtgttatgc gaccggccgg atcgcctccg
     4501 acccggcagc gctgctccgc tcccccaagc gtccgaaacg tctccccaag gccctcgagt
     4561 tgaccgcagc ggccgacctg atgggtctgc cgggcgtatc aagatggccc gagcgcgatc
     4621 aactcatcgt cgtacttgca ctcacgaccg gcctgcgtct cgaagagatg tcgacgctga
     4681 agatggcgga catctccggg gatcctcccg cgagcctcac cgtcatcgga aagggcaaca
     4741 aggagagagg ccttcctcta ccagcgatca cttcacatgc gatagaggca tacataccga
     4801 cgcgacgaaa gcgtctcgag aaactcggac tcgactccaa gaccttgttc atctcgacgc
     4861 gtccgcgcag ggtcggagcg cgcaaagacg ggagcgcgat catcagtgtc gacgcaactc
     4921 gggcgggtat cgcatatgtg gttgatcgga tgctcagcag gatcggtgcc cggcggcgag
     4981 gcagtcgcgt ccatgtactg aggcatacct tcgcaacgct gggcctccgg ccagacccaa
     5041 acaccggaca acctgcatat acgctgcgac agctgcagtc ggctctcggg catgccaacc
     5101 tctcgacgat ccaggtttat gcggaggtgt ccgacgcaga gctggtgcaa gcgtcggccg
     5161 cgcaccccct cagtcacgtg aaggttggtt caggggccga tcagtagctt tccgtcgata
     5221 tcggtgagac ccttcggaag ttcgagaagg accgtcgccc tgcgaacctg ccgggcgggg
     5281 atacgcccga tcgtgacagg gtaggagagt ctgcgttgac cgccgaggtt gagctgtacg
     5341 gtcccctgcc agggtctgtg tgtttggtta gtgatgatta cgatcatctc tatcttgcct
     5401 gaagttcccc ttgcggccag gtcgacgacg aggccctctt cgatacccgt agagggatca
     5461 gggatcgcgc atagagatga gtcgtcatcg cgcgtgaggg tgcgagagga agtgatcgtg
     5521 ggaacaccat cgggcaggat ggtgatggtg aacggtgttg ccaccgaact ggaacaggac
     5581 agaacgtgag taaggcccct caggccggag actccgatgg ggatgaggaa tgtggccagc
     5641 agtagtatca gggcgaaccg agctcgcagt agagatgaca tcggtcaagg ctgccaacgc
     5701 agatgcaact gtcgggcagc gcgcgcttcg tcgagtctcc cgacgggggt ggtcgtgggg
     5761 gcatccgtca gcaactcggg cgagctctcg gcctcatcgg cgatctgcag gagtgcgtcg
     5821 gccagcgcat cgagtgtctc tttggactcg gtctcggttg gttccgccat aagagcctct
     5881 tccaccacca atgggaagta aaccgtgggt ggatggaatc caaggtcgat caagcgcttc
     5941 gccacatcca tgccgcgcag gccttgcttg cggaactcct tcgctgtcgc gacgaactca
     6001 tgcatgcaca aaccggggtg cgccatcgga tagacagact cgatgcgcgc cctcaggtag
     6061 ttcgcgttca ggaccgctct ctctgataca cgatgaagcc caacggagcc cagagacctg
     6121 atgtacgtgt atgcgcgaag caccatagcg aagttcccat ggaatccgtg tatgtgtccg
     6181 atggagtcgg ggcgatccgc gtcccaacgg tatcgagagg tctgcgggtc atacgcgacc
     6241 agggggcctg gcaggaagcg ctccagccgt tgactcaccg ctacgggacc tgacccaggc
     6301 cctcccccac cgtgtggcgt ggtgaaggtc ttgtgcagat tcatatgcac gacatcgaac
     6361 cccatgtcgc caggacggat gatgcccatg atcgcgttca ggttggcgcc gtcgtaataa
     6421 aggagaccgc ccactctgtg gatgatcgcg gcgatcttct ctatgtcgcg ctcgaataga
     6481 ccaagcgtgt tggggttggt gagcatgagc ccagcgacgt tctcgtctgc tgcctcttct
     6541 agggcggtca gatcgaccaa accgttctca tccgatggga tctgacgaac ctgataaccg
     6601 gccagccgga ccgtcgccgg gttggtgccg tgggcggagt cgggtatgag aacatgcgtt
     6661 ctcgggcttc cttgggccgc atggaacgcg cgcatcagaa ggagccccgt cagctccccc
     6721 gcagcaccgg ccgcgggttg gaaagtaacc cgagccatgc ccgtgatctc ggcaagggcg
     6781 cgttccatct cccaaaggat ctgcagcgcg ccctgggtct gctccgcgct ctgccatgga
     6841 tgaagcgtcc ggaagcctgg aagcgcggcc atctgctcat gcaccttggg gttgtacttc
     6901 atggtgcagc ttccaagggg gtacgggcca acgtcgatgg cccagttcat gcgagagagg
     6961 ttggtgtagt ggcgcacgag gtcgagctcg gagacctcgg gcaggttggg ggcctgttgc
     7021 ctgcgaaact tcgatggcag ggagccgatg tgagccgagg cttcgacgcc ggacgcaggg
     7081 atactggagg cgcgccggcc cttcgaggac agatcgaaga tcgtgctcac gcgaccacct
     7141 cggccagcgc cttagcgaat ccctcgatct gttcgagggt tcttctttct gttacggcaa
     7201 cgatgatccc ctcgctacca tcggcgagca caacggcagg accagcgaga tatccgcgct
     7261 ccgagagatc tgagagcacc tgctcggctg gtctcggaag acgcaatacg aactccttga
     7321 agaagggcgc ttcgaaaacc agggaacatc ccggaacgct ggccagcagt tcggcggcgt
     7381 gggctgctcg cgccgagcag gtttcgccga gttcaacgag accctggggc ccgagccatg
     7441 cgagatacac agttgcgacg atggccataa gggtctggtt cgtgcagacg ttggagttgg
     7501 ccttctctcg gcggatgtgc tgctcgcgcg cctgcagtgt gagcacgaag cccagtttgc
     7561 ccgcaacatc cacggtctga ccacagatcc gcccaggtac gcgacgcaca tgttccatcg
     7621 ttgtcgcgaa gatgccgagg tatggccccc cgaagttgag gtcgttgccg agagattggc
     7681 cctcgccggt gacgacatcg gcgccgagtt tcccaggagc ttcgagggtt cccatcgcca
     7741 gaggatccat gtggaccacc atcaaggctc ctgccgcatg cacctgcgtc gcgaccgctc
     7801 cgacgtcttc gatgcatccg aagaagttgg gttgagccag gatcaagcag gccgcgtcat
     7861 cgatcgctgc aggctcggtt cgtccgcgcg gggcgggggc cgtgacgaag ttcagcccga
     7921 tacccgcgcc gaaagtctcg aggactttgc ggtagttagg gttgagtgcc tctgtgacga
     7981 cgatcttgtt gcgcccggtg gccccgactg cgaggttgac cgcttctacg agcgcagccg
     8041 ccccatcgta gaggctggcg ttcgcaacct ccatgcccga gagctcacag atcatcgttt
     8101 ggtattcgaa cagggcaccg aggacgccct gagatagctc tggctgatac ggcgtgtaag
     8161 aggtgacgaa ctccgaccgg taggctacag accgcacgac cgccgggatg tagtggtcgt
     8221 atgccccacc gccagcgaag catcgaagag agtccgtagg gatgttcttc gcggcgatct
     8281 gcgtgacgag atcagtgact tcggactcgg tcagttgatc gggtagatca agctctctgc
     8341 cgagccgcag gctcgatggt atctgtgaga agagatcttc gatgctggtc acacctatgg
     8401 tgtcgagcat cgaagcgaca tcaccgtccg tgtgtggcgt gtagtccatc tatctcctga
     8461 tgaatggggg cctggtaact tccatgtcgg ccctgcggcc gcgcacatcg acctggagca
     8521 tcgtacccgg agcggccacc tccgtggaga cgtaggccat ggcgatgccg cagcggatca
     8581 ccggtgagaa gttgccgctt gtgacatcac cgaccttttc gtcgtctcgg aagacctcat
     8641 acccctgacg agggatctca cgtcccttcg
//
LOCUS       DATDOZ010000091         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1127228, whole genome shotgun sequence.
ACCESSION   DATDOZ010000091 DATDOZ010000000
VERSION     DATDOZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127228"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VI541_01775"
     CDS             <1..1048
                     /locus_tag="VI541_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69664.1"
                     /translation="DPAGRYAAVPPDADSIRESCRHFWRVLHGDDVGKMILVSGFSIG
                     PSLKGVVRMRFNWPEQCDEAADWVFHEVSLKRDVFHRGYLSRPGGAILTSIYSELTAD
                     ELVAGPEPSIVVGSAPGLYQCYWKLAEPKPISAIRRLADRLLWRHDRLLRMPATDNHH
                     YHNSPSIGVVKHTAATFTVQELEECLPDCSGSVAIRSRDPVPGERSLVLEGPGSIFFA
                     MAESPTLRVSVAGAAIAALLLGGVVVPRITGVPHRSVGSVAAAVPFVRLESGVRPNYA
                     LGEVLASGKAPRSVPVAVAASVTDGRMRHPRERVPDAVAPITPDNLPTEVADLSDSAR
                     TTWQRAASVIWMVQ"
     gene            1045..1554
                     /locus_tag="VI541_01780"
     CDS             1045..1554
                     /locus_tag="VI541_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69665.1"
                     /translation="MRTLKVLLGIGLSMATLIGATLPAQAADTYRVGRALINLGHPAT
                     FAYSVTEFDFYVENRCAGVPKTQGVDGWVLSIDSLVDRYIVSTAASDHGVGLGFGVPS
                     NYAFYSADCGFVGPARNIEQGQPVPIPTNPQGVVLASWVVVSFPYGAPIYLDWKICTN
                     LNPATRCYF"
     gene            complement(1555..>2363)
                     /locus_tag="VI541_01785"
     CDS             complement(1555..>2363)
                     /locus_tag="VI541_01785"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HLF69666.1"
                     /translation="YVAFSSVRLGRRSIDVLMDRAPTGTISRIEETAAAVPGVAEVRR
                     VRFRYVGGQPQADIVVAISRSTPLESAHAVTEKVEEAIRALQPGTDVVVHVEPLADEA
                     AIADIVRMIAAREPRITDIHNVFVTSGSDGLHISFHIKMEGTMPLQDAHAISDQLEAS
                     IAKGIPGTARVDTHVEPLEMHESVIDVTADQNELVDWARSFAERQPEVRDCHEIVVTD
                     SDGDLTMVMHCHAGAGLAVTSIHEVATRIESAVHARWSAVKRVTVHFEPD"
BASE COUNT          433 a          710 c          705 g          515 t
ORIGIN      
        1 cgaccccgcc ggaagatacg cggccgtccc tccggacgcg gactccatca gggagagctg
       61 taggcacttc tggcgagttc tccatggtga cgatgtaggc aagatgatcc tggtttcggg
      121 attcagcatc ggccccagcc ttaaaggtgt agtgcggatg cgcttcaact ggcccgagca
      181 atgcgatgaa gcagctgatt gggtgttcca tgaggtatca ctcaagcgtg acgtattcca
      241 cagaggctac ctgtcgcgtc ccggtggcgc gatcctgaca agcatctact ccgaattgac
      301 ggccgatgaa ttggtagctg ggccggaacc atcgatagtc gtcggatcag cacccggcct
      361 ctatcagtgc tattggaagc tcgctgaacc taagcccata tccgcgatcc gtaggcttgc
      421 cgaccgtctg ctatggcgtc acgaccggtt gctgcggatg cccgcgaccg acaaccacca
      481 ctatcacaac tctccgtcga taggagtggt gaagcacacg gctgcgacgt tcacggtgca
      541 ggagctggaa gagtgcttgc ctgactgtag cgggtcagtc gcgatccggt cgcgcgaccc
      601 cgtaccgggt gagcgctcgc tggtgttaga agggcccgga tcgatcttct tcgcgatggc
      661 cgaatcaccg actttgagag tgtcggttgc aggggctgcg atagcggccc tgctgctagg
      721 aggcgtggtc gtgccgcgca tcacgggcgt gccccacaga agcgttggat cggtcgcggc
      781 ggcagtgcca ttcgtacggc ttgaatccgg cgttcgaccg aactacgcct tgggcgaggt
      841 actcgcatca ggcaaagcgc cacggtcagt ccccgtcgca gtggcagctt cggttaccga
      901 cggtcgcatg cgccatcctc gtgaaagagt gcccgacgca gtggctccta tcaccccgga
      961 caacctacca acggaggtag cagatctgag cgatagcgcg cgaacgactt ggcagcgcgc
     1021 cgcttcggtt atctggatgg ttcaatgagg acattgaagg tcctgctcgg gatcggcctt
     1081 tcgatggcca ccctgatcgg tgcgacctta ccggctcaag ctgccgacac ttacagagtt
     1141 ggtcgtgcct tgatcaatct gggacaccct gcaacgttcg cctactcggt aaccgagttt
     1201 gacttctatg ttgagaaccg ctgtgccggt gtaccgaaga ctcagggggt ggatggatgg
     1261 gttctgtcga tcgattccct ggtggacagg tatatcgtct caacagcggc atcagaccac
     1321 ggggtcggct tggggttcgg tgtgccctcg aattacgctt tctattccgc tgactgtggg
     1381 ttcgtcggcc cagcacggaa tatcgagcag ggccaacccg taccgatccc taccaaccct
     1441 caaggcgtcg tcttggcttc gtgggtggtt gtcagcttcc cttatggagc tccgatctac
     1501 ctcgactgga agatctgtac gaacttgaac ccggcgactc gctgttattt ctgatcaatc
     1561 gggttcgaag tgaactgtga cccttttcac agcggaccat ctcgcatgaa ccgctgactc
     1621 gatccgcgtc gcgacctcat ggatgcttgt aaccgccagt ccggcccccg cgtggcagtg
     1681 catcaccatc gtcaggtccc cgtccgagtc ggtcacgacg atctcgtggc agtctctgac
     1741 ctcaggttgt ctctcggcga atgatcgtgc ccagtcgacc agctcgttct ggtcggctgt
     1801 tacgtctatg acagactcgt gcatctcgag aggttccaca tgcgtgtcga cgcgagctgt
     1861 acccgggatg cccttcgcta tgctcgcctc gagttgatcg ctgatcgcat gcgcatcctg
     1921 gaggggcatc gttccctcca tcttgatgtg aaaggagatg tggagcccat ccgagcctga
     1981 tgtgacgaag acgttgtgga tgtcggtgat cctgggttcg cgcgctgcga tcatcctcac
     2041 gatgtcggct atcgcggctt catctgcgag cggttcaaca tgaaccacga catccgttcc
     2101 gggctgcaag gctcggatgg cttcctctac cttctcagtg accgcgtgag ccgactcgag
     2161 cggagttgac cttgagatgg cgaccactat gtctgcttgt ggctggccac caacgtaccg
     2221 gaacctgacc cgccgaacct cggcaacccc tgggaccgcc gccgccgtct cctcgatcct
     2281 gctgatagtg cccgtggggg ccctgtccat caggacatcg atactgcgtc ttccgagccg
     2341 gacgctcgag aaagcgacat aac
//
LOCUS       DATDOZ010000092         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2173381, whole genome shotgun sequence.
ACCESSION   DATDOZ010000092 DATDOZ010000000
VERSION     DATDOZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173381"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..704)
                     /locus_tag="VI541_01790"
     CDS             complement(87..704)
                     /locus_tag="VI541_01790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF69667.1"
                     /translation="MDLRSHWQEVFKTKEADQVSWFQPHLRLSMQLIQRAGISAESHV
                     IDIGGGDSTLVDDLLDQGVGEVSVLDISSEALARAKRRLGPLSDKVIWIEADATKAQL
                     PSSHYDVWHDRAAFHFLTDPADRGAYLEILGRAIRPSGHVIIATFAPDGPERCSGLAT
                     VRYSPESLQAELGPRYELVAAELEAHQTPFATEQRFVYCLFKALD"
     gene            complement(722..1009)
                     /locus_tag="VI541_01795"
     CDS             complement(722..1009)
                     /locus_tag="VI541_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69668.1"
                     /translation="MKKFMLLHFGFEMPTPEIMAAWEAWFESISNRQVDQGGFAGGRE
                     ISKDGTKDLAWDIDSITGYNIIEAESLDDAEKLAQSNPFIASIRIYELRSK"
     gene            1149..>2016
                     /locus_tag="VI541_01800"
     CDS             1149..>2016
                     /locus_tag="VI541_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HLF69669.1"
                     /translation="MSKIQEYLRYLVEEQASDLHLKVGAPPIIRISGRLRRSNFEVLG
                     ADDTERAAMEIMSEMTARQFQESGEADFAYSTPGLGRFRCNVYRQRGSIGIAIRRVLP
                     GGASFESLGLPPVVKRISDSPRGLILVTGATGSGKTTTTGAIIHHINETRQVHIMTIE
                     DPIEILHADKKAIVNQREIGIDTQGFAQAMKRAMRQDPDVIFIGEMRDQETVHAALSA
                     AETGHLVISTLHTTDATETINRIIDFFPPHQQKQVRVTLAASLRGIISQRLIRKKDGE
                     GLVPAVEIMVMNG"
BASE COUNT          402 a          599 c          610 g          405 t
ORIGIN      
        1 gttgaagatg cgccatcaaa ccccctatcg aacatggccc tcgatcactt gatcagggct
       61 cactccgctc gttggcggct gggggcctag tcgagggcct tgaacaggca gtagacgaat
      121 ctctgctcag tagcgaaggg ggtctggtgt gcttcgagct ccgctgcgac gagttcgtag
      181 cgcgggccca actcggcttg aaggctttcc gggctgtacc gcacggtcgc taggccgctg
      241 catcgctctg ggccatccgg cgcgaaggtc gcgatgatga catggccgga tggccgtatg
      301 gcccgaccca atatctcgag gtaagcacct ctatctgcag gatccgtcag gaagtggaag
      361 gccgccctat cgtgccagac gtcgtagtgc gaagaaggaa gttgtgcctt tgttgcatcc
      421 gcttcgatcc aaatgacttt gtctgagagc gggccgagcc tgcgcttggc gcgggccagg
      481 gcttccgagg agatgtccaa gacgctcacc tcgcccacgc cttggtcgag caggtcgtcg
      541 accagcgtcg aatctccacc gccgatgtcg atgacgtggg actctgccga gatgccagcg
      601 cgttggatca actgcatcga aagacgaaga tgtggctgga accagctgac ctggtcagcc
      661 tccttcgtct tgaagacctc ttgccaatgc gacctcaagt ccacgtcagg actcctcaca
      721 cctacttgga ccgcaattcg tagattcgaa tgctagctat gaatgggttg ctctgggcta
      781 gtttctccgc gtcgtcgaga ctctctgcct cgatgatgtt gtagccagtg attgagtcga
      841 tatcccaagc taggtccttg gttccatctt ttgagatttc tcgaccacct gcgaaaccac
      901 cctggtcgac ctgcctgttg gagatcgatt caaaccaggc ctcccaggcg gccatgatct
      961 cgggcgtggg catctcgaag ccgaaatgca gcaacatgaa ctttttcaag tccgctcctt
     1021 tgtttgaggg cgcgctgcat cctagccact ttggtcaacg gattggcggt cagcgggttg
     1081 gaccgacttc cagggtagga acgtggctcg cggtatcctg ctggggtcat tcgagccgtg
     1141 gaggcgacgt gtcgaagatc caggagtatc tgcgctatct ggttgaggag caggcatccg
     1201 accttcatct gaaggtgggg gccccaccga tcatccgcat cagcggtcga ctgcgccgct
     1261 cgaacttcga agtccttggg gccgatgaca ccgagcgagc tgcgatggag atcatgtccg
     1321 agatgaccgc ccggcagttc caggagagcg gcgaggcgga cttcgcctac tccaccccgg
     1381 gcctagggcg cttccgctgc aacgtctatc gccaacgtgg gtcgatcggt attgcgatcc
     1441 gcagggtgct gccgggcggg gcgagtttcg agagcctggg gctccctccg gtggtcaagc
     1501 ggatatccga ttcaccccga ggtttgatcc tggtaacggg ggccacgggc tcgggcaaga
     1561 ccacgacgac cggggcgatc atccaccaca tcaacgagac gcgccaagtc cacatcatga
     1621 ctatcgagga tcccatcgag atactccatg ctgacaagaa ggccatcgtc aatcagcgag
     1681 agataggtat cgatacacaa ggcttcgcgc aggctatgaa gagagcgatg cgccaggacc
     1741 ccgatgtcat cttcatcggc gagatgcgcg atcaagagac cgtccacgcg gccctgagcg
     1801 ctgccgagac cggacacctc gtcatctcga ccctccacac gaccgatgcc accgagacca
     1861 tcaacaggat catcgatttc ttcccgccgc atcagcagaa gcaggtacgc gtgactctgg
     1921 cggcatcgtt gcggggcatc atcagccaac ggctcatccg caagaaggac ggtgagggtc
     1981 tggtcccggc ggtcgagatc atggtgatga acgggc
//
LOCUS       DATDOZ010000093         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3745156, whole genome shotgun sequence.
ACCESSION   DATDOZ010000093 DATDOZ010000000
VERSION     DATDOZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745156"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VI541_01805"
     CDS             <1..1123
                     /locus_tag="VI541_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69670.1"
                     /translation="PCVSDWGARMPRQISPTVLVACDEPDLSEEIVRYLEEIPGWILV
                     GSARSLDELTSSETSPDAVLLSDTLAGQLAVSSPDLLSRSHVVVIGRQETSDALKAAL
                     WLGARGFVNWPAERRTLRKLVETGLRDRSPAAHMGPLTSLWAPKGGSGSSVLAAHLAG
                     AIAPISGCVLVDLDLDHSDQSVILGSDADVKGALDLLRIVDEISADAIQGVSWTHPAG
                     FRAILSRGAPGESDLVKSSDIVKMIQAIRETADHVIVDLPSGYGESVLAVAEQSSRLL
                     LVVTPDLLSLHRARDAMAMLRSAGIDSEHIEVLLNQYSGGDVRVGDVEGVLGRPVIHK
                     VRPDAGLFRSAGRGELSQRGIKQMRALASVITGPPTAVS"
     gene            1244..2725
                     /locus_tag="VI541_01810"
     CDS             1244..2725
                     /locus_tag="VI541_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLF69671.1"
                     /translation="MSEESFERNGDYGALFFEGETYRTGRIFDGAVRAATGLTELGVR
                     KGDRVVIVMANCPEVPVAYGATWRAGAAITPAMFLLPPDEIRRIIEDSGATAVITTPE
                     FLANVQTAAKDLPDVLAIISTGPPTEGVVSLDALTKADTSPMVATDADDLAAILYTGG
                     TTGQSKGVMLTHENLRVMARGAFEASDVIDGDRAITALPLAHGYGMMSMVAASHGNHG
                     YAVLMRWFDPSQWPALVQDHKIRRAAVVPTMLQLLLSQPLEDYDLSSLEVVNSGAAPL
                     AKETILEFERRVPSCLILEGYGCSESGVITSVNRRDHRKIGSVGLPFPGYEVKCVDDD
                     GKDVNLDEVGEICVRSGGVMEGYWKSPELNAEVLTDGWLHTGDIGKVDSDGFVWIVDR
                     KKDLIIRGGFNVFPRDVEDALLEDPAVEMAGVVGRPDKIYGEEVVAFVSLRSGASATP
                     EEIIERSKTKLGKYKYPREVHVVGYLPLTPVGKVDRKALRNML"
     gene            2742..>3674
                     /locus_tag="VI541_01815"
     CDS             2742..>3674
                     /locus_tag="VI541_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLF69672.1"
                     /translation="MSAIDNELDLTPVRDLADVDIDTVYEHMDQQMRDRPTPLELYAI
                     WERGNWRVSDLDFSEDKIHWGLLLPELKDYLQRVFTEFFLGEQAVTDTLSPLVHAAPD
                     EDSRIFLSTQIADEARHTVFFKRFFAEVVEVGGGLSDALASLRPEAVEGFRKIFDEDL
                     VAATERCREDPTDRAAWVRGITIYHLVVEGMLALTGQKYLVRVFRDLGMLPGFRAGFT
                     AVARDESRHVNFGVGALANQIKLNPAMADEVAGAVRGILRAACATVEPADRDYENITH
                     PNDFPPQARINPREFHRFSLTSLTKRLRVAGLSAD"
BASE COUNT          665 a         1125 c         1159 g          725 t
ORIGIN      
        1 cccatgcgta tcggattggg gggcacgcat gccacgacag atctcgccta ccgtgctcgt
       61 cgcctgcgat gagcctgatc tctctgaaga gatagttcgc tatctcgaag agataccggg
      121 gtggatcctc gtcggatcag ctcgttcact cgatgagttg acgagcagtg aaacctcacc
      181 cgatgccgtc cttctatccg acactctcgc ggggcagctg gcagtgtctt cgcccgacct
      241 gctgagccgt tctcatgtgg tcgtgattgg tcggcaggaa acgtcggacg ctctcaaggc
      301 cgctctgtgg ttgggggccc gtggcttcgt caactggcca gccgagaggc ggacgctgcg
      361 caaacttgtc gagacgggcc tgcgtgatcg gagcccagca gcacatatgg gaccactcac
      421 ttcactgtgg gcacccaagg gcggaagtgg gagcagcgtt ctggccgccc atctcgcagg
      481 agccatcgcc ccgatctctg gttgcgttct cgtcgatctc gatctggatc actcggatca
      541 gtcggtcatc ctcggctctg acgctgacgt gaagggagct cttgaccttc tgcggatcgt
      601 cgatgagatc agtgcggatg cgatccaggg cgtcagttgg acccaccccg cgggcttccg
      661 cgcgatcctg tcgcgcggcg ctccgggtga gagtgacctg gtgaagtcct cggacatcgt
      721 gaagatgatc caggccatcc gcgagactgc cgatcacgtc atagtcgatc ttccttcggg
      781 ctatggcgag tcggtcctcg ccgtcgccga acagtcctcc cggctcctgc tcgtggtaac
      841 ccccgatctg ctcagtctgc accgcgcgcg cgatgcgatg gccatgctga ggtccgccgg
      901 catcgactcg gagcacatcg aggtgctcct caatcaatat tcgggcggtg atgtccgggt
      961 cggcgatgtc gagggggtgc tagggcgccc cgtcatccac aaggtgaggc ccgatgccgg
     1021 cctgttccgt tccgcgggcc gaggggagct ttctcagcgg ggcatcaagc agatgcgagc
     1081 cctagcatcg gtgatcacag ggccccccac ggccgtctca taggagaaaa ccacctgtgc
     1141 ggtggttggt tgctataaga ccagagccga acaggggctg gcgcgaggat aatcgtctct
     1201 cacggataag gagatagcca tcgcgacctt caacgtagct cagctgagcg aagagtcatt
     1261 cgaacgtaat ggcgattatg gggccctctt cttcgaggga gagacctacc gtacgggcag
     1321 gatattcgac ggggcggtca gggccgccac ggggctcacc gagttgggtg ttcgaaaagg
     1381 tgaccgcgtc gtcatcgtga tggcgaactg tcccgaggtt ccggtcgctt acggcgcgac
     1441 gtggcgagct ggcgcggcga tcacgccagc gatgttcctg cttccgccgg acgagatccg
     1501 tcgcatcatc gaggactccg gtgctaccgc ggtcatcacc acgcctgagt tccttgccaa
     1561 tgttcagacc gcggcaaagg acctccctga tgttttggcg atcatctcta cggggccccc
     1621 gaccgagggc gtggtatcgc tagacgcgct caccaaggcc gacacgagtc cgatggtcgc
     1681 aaccgatgct gatgacctcg ccgcgatcct ctacacaggc ggaaccacag gtcagtccaa
     1741 gggcgtgatg ttgacccacg agaacctgag agtgatggcg cgcggagcgt tcgaagcttc
     1801 cgatgtcata gatggcgacc gagccatcac cgccctgccg ctggcgcatg gctacgggat
     1861 gatgagcatg gtcgccgcca gtcacgggaa ccatggttac gcggtgctga tgcgctggtt
     1921 cgatccatcg cagtggcctg ccctcgtgca ggatcacaag atccgtcgcg ccgctgtcgt
     1981 tcccacgatg cttcagctgc tcctgtcaca accgctcgag gattacgacc tatctagtct
     2041 cgaagtggtg aactcggggg ccgcgccgct ggccaaggag acgatcctcg agttcgagcg
     2101 ccgcgtccca tcatgtctga tcctcgaggg ttacggctgc tccgaatccg gggtgatcac
     2161 ttcggtcaac cgtcgagacc accgaaagat cgggtcggtc ggccttccct tccccggcta
     2221 cgaggtgaag tgtgtcgacg atgatggcaa ggatgtgaac ctcgatgagg ttggcgagat
     2281 ctgcgtccgc tccggcggtg tgatggaggg ttactggaag tctcctgagc tgaacgcaga
     2341 agtcctcacg gacgggtggc tccacaccgg tgacatcggc aaggtcgaca gtgacggttt
     2401 cgtctggatc gtcgatcgca agaaggacct gatcatcaga ggagggttca acgtcttccc
     2461 gcgagacgtc gaagatgccc tactcgagga ccctgctgtc gagatggcag gcgtcgtggg
     2521 gcgccccgac aagatctacg gcgaggaggt cgtggccttc gtctcgttgc gatcgggggc
     2581 ctcggctact cccgaagaga tcatcgagcg ttcgaagact aagcttggta agtacaagta
     2641 cccgcgcgag gtccatgtcg ttggctactt gcctttgaca cccgtcggga aggtggaccg
     2701 caaggccctc cggaacatgc tctgaaaggg aggctgcatc gatgagcgcg atcgacaacg
     2761 aacttgatct gaccccggtt cgcgacctgg ccgatgtcga tatcgacacg gtctatgagc
     2821 acatggatca gcagatgcgc gaccgcccga ctcccttgga gctatacgcg atctgggagc
     2881 gggggaattg gcgcgtttcg gatctggact tcagcgagga caagatccac tggggccttc
     2941 tgcttcctga actcaaggat tatctccagc gagttttcac cgagttcttc ctcggcgagc
     3001 aggcagtcac cgacacgctc tcgccgctcg tccatgccgc ccccgatgag gattcgcgca
     3061 tcttcttatc gacgcagatc gcagacgagg cgcggcacac cgtcttcttc aagaggttct
     3121 tcgccgaggt cgtcgaggtc ggcgggggcc tgagcgatgc cctggcgagt ctgcggcccg
     3181 aagccgtcga ggggttccgc aagatcttcg acgaggacct cgtcgccgca accgagcgct
     3241 gtcgtgagga tcccaccgac cgagccgcct gggtccgcgg gatcaccatc taccacctgg
     3301 tcgtcgaggg gatgttggcg ctcaccggcc agaaatatct cgtcagggtc tttcgcgacc
     3361 tcgggatgct ccccggtttc cgggcgggtt tcacagcagt cgctcgcgac gagtcgcggc
     3421 atgtgaactt cggggtcggt gcactcgcca accagatcaa gctcaacccg gctatggccg
     3481 atgaggtcgc cggtgccgtc cgcgggatcc tgcgggcagc ctgcgcgacc gtcgagcccg
     3541 cagatcgtga ctacgaaaac atcacgcatc cgaatgactt cccgcctcag gctcgcatca
     3601 acccccgaga gtttcaccgt ttctcgctga cttcacttac caagcggctt cgcgtcgcgg
     3661 ggctttcggc cgac
//
LOCUS       DATDOZ010000094         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1128360, whole genome shotgun sequence.
ACCESSION   DATDOZ010000094 DATDOZ010000000
VERSION     DATDOZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128360"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..397
                     /locus_tag="VI541_01820"
     CDS             137..397
                     /locus_tag="VI541_01820"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HLF69673.1"
                     /translation="MTKRYDEPIEVEVSRLQPVAFSWRGRRYPVSRLLKYWREAGAGW
                     DPERLRDHECFRVESEGGTYDLRHERRSSRVATSWRLLRVWD"
     gene            521..>2801
                     /locus_tag="VI541_01825"
     CDS             521..>2801
                     /locus_tag="VI541_01825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLF69674.1"
                     /translation="MPQFTHLHVHSNYSLQEGAAYVDDLAQTAARIGLESLALTDHDG
                     MYGAVRFTVACRQAGVRPILGAEIEYGEGFHTVLLAKDAIGWSNLCRLVTQMHLSDTS
                     RDARARPRTTFESIANHSEGLIALSGCARGEVAWYASLGRSDEAASAARRFVDVFGSQ
                     RFAIEVSNHLLEEDPLRIRRLRDIARDLGLRTVATNNVHYVGPQDAVLHDLADCMRRL
                     VALTPASAPRRNNEYWLKSEAMMAQLHDPAAIEGAAWVAEQCAFEFDLGSFHFPDIEN
                     AQGDSSTSVLARRCWEGARRRYPVISHKVQERLQTELQMIRRGGFCGYFILVADIVDH
                     ARNDLGIRAACRGSAAGSLVAYLLGISEVDPIRYDLVFERFMNEHRREIPDIDVDFES
                     ARREEMNAYILGRYPGRTAMAGMMDTYRARGALREVGKALGIDGSEVDLICKTFPHIS
                     ARAIPAALEALPEVAEMGLRSSKLDMLFELASRIDAYPRHLAMHPSGVILSDSKLADL
                     MPMQRSFNGFLMSQFDKDDIETLGLAKLDVLGVRMFSTISYALDEIERVHGVRVDIDA
                     VPRDDERTFELIRDSRTMGCFQIESPGQRELLGKFQPECFEDLIIDISLFRPGPMKAD
                     MIKPFLNRRMGLEPVTYLNRLLVPILRESNGVVVYHEQVMRVIAALTGCTLGEADKIR
                     RSLDGDRHDQGEHPLTAWVLERAIERGWDETCARRVWSEALSFASFGFCKAHAAAFAV
                     PTYQSAWLKAHHPAEFYCGV"
BASE COUNT          533 a          858 c          868 g          542 t
ORIGIN      
        1 gttctgttcg ctcatcccca agcccccata tgtttgagga agaaaatgtg acacacgggg
       61 ttgacatact caatcgaaca ggtgtacggt tacttcccgc ccgaacgtat gttcgggcca
      121 gagagggaga ccctgcatga ccaagaggta cgacgagccg atcgaggttg aggtgagcag
      181 actccagccc gtcgccttca gctggcgtgg tcgtcggtat cccgtgagca gactcctcaa
      241 gtactggcgc gaggcggggg ccggttggga cccggaacgt cttcgtgatc acgagtgttt
      301 cagggtcgag tccgaagggg gtacctatga cctgaggcat gagcgaagaa gcagccgggt
      361 cgcaacgagc tggcgtctgt tgagggtgtg ggattgacgg tgggcgtcac agactgaact
      421 ctccgtgcgc tcccatcatg tttggggcgc ccaccgttca caccttcaac cgacatcagc
      481 tccccatggg ctcccggccc ccgaacgcgg gcacaagacg atgccgcagt tcactcatct
      541 acacgttcac tcgaactact cgcttcaaga gggcgcggcc tacgttgatg atctcgcgca
      601 gaccgcagcg cgtatcggct tggagtctct cgccctaacc gaccacgacg ggatgtacgg
      661 agcggtccgc ttcacggtcg cgtgcaggca ggcaggggtg cgtccgatcc tgggggccga
      721 gatcgagtac ggcgaaggtt tccacacggt ccttctcgcg aaggatgcca tcgggtggtc
      781 gaacctctgc cgactggtga cgcagatgca tctcagcgac accagtcgag atgcgcgggc
      841 acggccaagg acaacgttcg agtcgatcgc gaaccacagc gaaggtctga tcgcacttag
      901 cgggtgcgcg cgcggtgagg tcgcctggta cgcatcgctg ggccgcagtg acgaagcggc
      961 ctcagcagcc cgtcgcttcg tcgatgtctt cggctctcag cgattcgcta tcgaggtcag
     1021 caaccacctt ctcgaagaag acccgctgcg catccgccga ctacgagaca tagcccgtga
     1081 tctgggcctg cgcacggttg cgaccaacaa cgtccactat gtcggccccc aggatgcggt
     1141 cctgcatgac ctggccgact gcatgcgacg tctcgtagcg ttgacccctg cttcggcccc
     1201 ccgccgtaac aacgagtact ggttgaagtc cgaggcgatg atggctcagc tccacgaccc
     1261 agccgcgatc gagggggccg cgtgggtggc agaacagtgc gccttcgagt tcgaccttgg
     1321 ctcattccac ttcccagaca tcgaaaacgc tcagggggac tcctctactt cggtcctggc
     1381 tcgcaggtgc tgggaggggg cccgtcgacg ttatccggtc atctcgcaca aggtgcagga
     1441 gcgactccag acggagcttc agatgatccg tcggggtggc ttctgcggtt acttcatcct
     1501 ggtcgccgac atcgtcgatc atgctcgcaa cgacttgggg atccgtgcgg cctgccgggg
     1561 gagcgcggcc gggtcactcg tcgcgtatct gctcggtatc tcagaggtcg accccatccg
     1621 ctacgacctc gtcttcgaac ggttcatgaa tgaacaccgt cgcgagatcc ccgacatcga
     1681 cgtcgacttc gagtcggccc ggcgtgaaga gatgaacgcg tacatcctcg gtcgttaccc
     1741 cggacgtacc gcgatggcgg ggatgatgga cacctaccgg gcccgcggag cgttgagaga
     1801 ggtcggcaag gccctcggta tcgacgggtc agaggtcgac ctgatctgca agaccttccc
     1861 acacatatcc gcacgggcga taccggcagc gctcgaagct ctgcccgagg tcgccgagat
     1921 gggacttcgt tcgtcgaagc tggacatgct cttcgagctg gcttcgagga tcgatgctta
     1981 cccgcgacat ctggcgatgc acccatcggg ggtgatcctg tcggattcga aactcgccga
     2041 cctcatgccg atgcagcgtt cgttcaatgg attcctcatg tctcagttcg acaaggacga
     2101 catcgagacg ctggggctgg cgaaactcga tgtcctcggc gtgaggatgt tctctaccat
     2161 ctcttacgcc ctcgatgaga tcgaacgtgt tcatggcgtg agggtcgata tcgatgcggt
     2221 ccccagggac gacgagagga ccttcgagtt gatacgcgac tcgcgcacga tgggatgttt
     2281 ccagatcgag tccccaggcc agcgcgaact tctgggtaag ttccaacccg agtgcttcga
     2341 ggacctcatc atcgacatct ccctcttccg gccggggccg atgaaggccg acatgatcaa
     2401 gcccttcttg aaccgcagga tgggtctaga gccggtcacc tatctgaaca ggctgctcgt
     2461 gccgatcctt cgagagtcga atggagtcgt cgtctatcac gaacaggtga tgcgagtgat
     2521 cgccgctctg acgggatgca cgctcggcga ggccgacaag atcaggcgca gtctcgacgg
     2581 cgatagacac gatcaaggtg agcacccttt gaccgcatgg gtcctcgaac gagcgatcga
     2641 gcgcggatgg gatgaaacct gcgcgcgcag ggtctggagc gaggctctct ccttcgcttc
     2701 attcggcttc tgcaaagccc atgccgcggc gttcgcagtt ccgacctatc aatcggcgtg
     2761 gctcaaggcc caccaccccg ccgagttcta ctgcggggtc c
//
LOCUS       DATDOZ010000095         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2175053, whole genome shotgun sequence.
ACCESSION   DATDOZ010000095 DATDOZ010000000
VERSION     DATDOZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175053"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VI541_01830"
     CDS             complement(<1..533)
                     /locus_tag="VI541_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HLF69675.1"
                     /translation="MNRLLKNPITYFLVIIVAIWVVASVLKQGAGPKHLDYGTFKTKL
                     ESGQVDSVVAFKNGGKLEGELTDGTKFRSEFGSLDKLEEAFDKAGKSEILQFKNDTPS
                     FWGNLIGSILPFIIIIGIFLFLMQQMQGGGNRVMSFGKAKAKVVSKDQPKITFGDVAG
                     VDEAIEELEEIKEFLENP"
     gene            complement(597..1124)
                     /gene="hpt"
                     /locus_tag="VI541_01835"
     CDS             complement(597..1124)
                     /gene="hpt"
                     /locus_tag="VI541_01835"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HLF69676.1"
                     /translation="MFESEIGEILIDEHEIQDKIRELAKNITEDYLDKDLLLVGVLKG
                     AFLLMSDLAREIKLPLEFDFMAVSSYGSTTKTSGVVRILKDLDYEITDRNVLLVEDII
                     DSGLTLNYLLKYLRARRPASLEVCALLRKDGIQQVDTHVRYEGFVIPPKFVIGYGLDY
                     NQRHRNLPYVALLKE"
     gene            complement(1175..>2032)
                     /gene="tilS"
                     /locus_tag="VI541_01840"
     CDS             complement(1175..>2032)
                     /gene="tilS"
                     /locus_tag="VI541_01840"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLF69677.1"
                     /translation="MIDLRREAAVTVCERAGLPFVTDPSNTDERFTRNRIRRELRERG
                     EEHLLGLVELGRRNAESVTDVDREVEALLADIATERDDLIVLDATRLEHLPETTARAV
                     LRRAMTNWDPEPTSPSLARALDVAANGGRASLGADVHIWREGESVFMGAWPQRVELPE
                     LVLPVPGHIVSGQWGWEFRSRADYPSYPVVIRPRTERFDADALEDVVTVRQWRPGDRM
                     TPLGLSGSKKLQDLFTDLKIPRRERDRVPVIESGGEIVWVVPFRIAEPFKVTAATRRV
                     MTITAIREA"
BASE COUNT          398 a          635 c          580 g          419 t
ORIGIN      
        1 gggttctcga ggaactcctt gatctcttcc agttcttcga tggcctcatc tacccctgcg
       61 acatcgccga acgtgatctt cggctggtcc ttcgagacga ccttggcctt ggccttgccg
      121 aacgacatca cgcggttgcc gccgccctgc atctgctgca tcaggaagag gaagatcccg
      181 atgatgatga tgaacggaag gatgctcccg atcaggttcc cccagaacga cggcgtgtca
      241 ttcttgaact ggaggatctc ggacttgccc gccttgtcga aggcctcctc gagcttgtcg
      301 agggaaccga actcggaacg gaacttggtt ccgtcggtca gctcgccttc aagtttgccg
      361 ccgttcttga aggccaccac cgagtcgacc tgaccggatt ccagcttggt cttgaaggtt
      421 ccgtagtcga ggtgcttcgg ccctgcaccc tgtttcagga cgctggcaac cacccagatg
      481 gccacgatga tcaccaggaa gtaagtgatg gggttcttga ggagcctgtt cacagtcaag
      541 tcagcgtacc accggacccc atcgtttggt caggtcagat ctgccggcag gggccgctac
      601 tccttgagca gggctacgta cgggagattt cggtggcgct ggttgtagtc gagcccataa
      661 ccgatgacaa acttgggagg gatcacgaaa ccctcgtatc tgacgtgggt atcgacctgc
      721 tggatgccat ccttgcggag cagtgcgcag acctcgagtg aggcgggcct tcgcgccctc
      781 agatacttga gcaggtagtt gagggtcagc cccgagtcga tgatgtcctc gaccaggagc
      841 acatttcgat cggtgatctc gtagtcgagg tccttgagga tccgaaccac tcccgatgtc
      901 ttcgtcgtcg atccgtagct cgaaacagcc atgaagtcga attcgagcgg cagcttgatc
      961 tcacgggcga ggtcactcat cagcaggaat gcccccttga gaacgcctac gagcaggagg
     1021 tccttgtcta gataatcctc ggtgatgttc ttggcgagct cgcggatctt gtcctggatc
     1081 tcgtgttcat cgatgaggat ctcgccgatc tcggactcaa acatggggag ggccatagta
     1141 aggcgcatca cctatcaacc gcaagatgac cccatcacgc ttctctgatc gccgtgatcg
     1201 tcatgacccg tcgcgttgcc gccgtcacct tgaagggctc ggcgatccgg aatgggacaa
     1261 cccagacgat ctccccccca gactcgatca ccggcactct gtcgcgctct cgccgcggga
     1321 tcttgaggtc ggtgaagaga tcctgaagct tcttgctccc ggagagtccg agcggagtca
     1381 tgcggtcacc tggacgccac tgcctgacgg tcaccacgtc ttcgagcgca tcagcatcga
     1441 atctctccgt tctcgggcgg ataaccaccg gatatgaggg gtaatccgcc cgagaacgga
     1501 actcccaccc ccactggcca gagacgatgt gacctgggac cggaaggacc aactcaggca
     1561 actcaacccg ctgaggccag gcccccatga acacagactc accctcgcgc catatatgga
     1621 catccgcccc cagactcgcg cgtcccccat tggctgcaac gtccagcgcc cgcgcgagcg
     1681 atggtgatgt cggttcaggg tcccagttcg tcatcgcccg gcgcaacacc gccctcgcgg
     1741 tcgtctcagg cagatgttct aggcgggtcg cgtccaggac gatcaggtca tctcgctccg
     1801 ttgcgatatc cgcgagaaga gcttcgacct cgcgatccac atcggtgacc gactctgcat
     1861 tccgacggcc aagttcaacc aatccgagca gatgctcctc gccgcgctca cgcagttcac
     1921 ggcggatgcg attgcgagtg aatcgctcgt cagtgttgga cgggtcggtc acgaatggga
     1981 gcccagctcg ttcgcatacg gttaccgccg cctcgcgacg cagatcgatc at
//
LOCUS       DATDOZ010000096         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3468324, whole genome shotgun sequence.
ACCESSION   DATDOZ010000096 DATDOZ010000000
VERSION     DATDOZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3468324"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="VI541_01845"
     CDS             <1..1935
                     /locus_tag="VI541_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69678.1"
                     /translation="ITPSGGPEGGPAPKKASQAEIDALARPGGGRGGWITSMPVTPDL
                     LAKVQALPEANDEPRVRVEDAIADTGGFRIRELLKGFRGGIILGLLLLMASTVLGLLG
                     PSFSKWGIDGTLTPTKAALAYLVAVIVQGFVMWASMIKTLGIGESALYKLRLKVFSKL
                     QRTSIDYYDREMSGRIMSRMTSDIDSFGTFVQEALFNILSSFLRLAGVVVILLLTEWH
                     LALVVSLGVLPLLLGFTQWFRRRSDRVYLKVRDRIAAVLASLQEGIAGVRISQAFVRE
                     KEESAQFRKLSTELLEAREEGVRYLSLFFPGIDAIGVLAQAAVIGTAGYLITGGSLTA
                     AVVVAFILYLNQFFGPIQQLSQLFDTYQQAKAAGRKLDDVLARPSSTPAPDAPVTLGS
                     AQGIVGFEDVRFGYHEAAEVLHGVDLQISAGERVALVGKTGAGKSTLLKLASRFYDPT
                     GGRVLIDGVDLRSVESHSLRRAVGVVPQDPFLFTGSIRDNIVYGRPDATDAEVYEAAR
                     LVGAHDIISTSLKGYDTHIKGRGRGLSAGEKQLIALARVALVDPKILLLDEPTSRLDL
                     QTEAQILTALDRLLDGRTSLIIAHRLSTIRNADRIVVLDEGRIVEQGPHAELLDRRGL
                     YSEMHERWLGEAPEPVAQVS"
     gene            complement(1928..>2668)
                     /locus_tag="VI541_01850"
     CDS             complement(1928..>2668)
                     /locus_tag="VI541_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69679.1"
                     /translation="KLIAFFVLGSLLPTAGLIYLALNGNELGALNATGRIDLATIASL
                     GIMQTFALIATLAVSMNNVGPGFASGEAAMILARPLGRARYAMGRFIASSSITVGLCA
                     LLAVETSLPGLLTGQHRFALLWEHWGTVAFNLVVVGAITTMLSALVNPAALAAIAAYI
                     FQAGAGAVASIRGLVTGGLLKGFLADILNLAWYVTPKFLTSPLSNQQIAGIITLPRNT
                     LGLTVWAIAYAAGALAVTAWVVQRKRVS"
BASE COUNT          499 a          810 c          851 g          508 t
ORIGIN      
        1 ataacgccga gcgggggccc cgaagggggg ccagccccca agaaggcgag ccaagccgag
       61 atcgatgcct tggctcggcc cggcgggggg agagggggct ggatcacttc catgccggtc
      121 acccccgatc tgttggcaaa ggtccaggcc cttccggaag ccaatgacga accgcgagta
      181 agggtcgaag atgccatcgc cgacacgggt ggttttcgga taagagagct tctcaaggga
      241 tttcgcgggg gcatcatcct tggactgttg ctcctcatgg cctcgacggt tcttggcctg
      301 ctgggtccgt cgttctcgaa gtggggtatc gacgggacgt tgacacccac gaaggctgcc
      361 ctggcgtatc tggtcgcagt gattgttcag ggcttcgtca tgtgggcctc gatgatcaag
      421 acactcggca tcggcgagag cgcactgtac aagttgaggc tgaaggtctt ctcgaaactc
      481 cagagaacat ccatcgacta ctacgaccgc gagatgtcag ggcgcatcat gagccgcatg
      541 acgagtgaca tcgactcgtt cggaaccttc gttcaggagg ccctgttcaa catcctctcg
      601 agtttcctgc gtctcgctgg cgtggtggtg atcctcctgc tgaccgagtg gcatctggct
      661 ctcgtggttt ctcttggggt cctgccgctg ctgctcggat tcactcagtg gttcaggcgg
      721 cgctcggaca gggtgtatct caaggtccgc gaccggatag cggccgtact cgccagtctc
      781 caggaaggga tcgcgggggt ccgcatatcg caggcattcg tgcgcgagaa ggaagagtcg
      841 gcgcagttca gaaagctatc gaccgaactc ctcgaggccc gcgaggaggg tgttcgctat
      901 cttagtctct tcttccctgg catcgacgcc atcggtgtac tcgcgcaggc ggcggtcata
      961 ggaacagcgg gatatctgat cacgggcggc tcgctgacgg cagcggtcgt cgtcgcattc
     1021 atcctctact tgaaccagtt cttcggcccc atccagcagc tgtcgcagct cttcgacacc
     1081 tatcaacagg ccaaggccgc aggccgcaaa ctcgatgacg tactggctcg accgagctca
     1141 accccggccc ccgacgcgcc ggtgacgctg ggatctgctc agggcatcgt aggtttcgag
     1201 gatgtgcggt tcgggtatca cgaggccgct gaagtgctgc atggtgttga tctccagatc
     1261 tcggctggcg agagggtcgc actagtgggc aagaccggtg ctggcaagtc gactcttctc
     1321 aagctggcct ctcgcttcta cgacccgacc gggggccggg ttctgatcga cggcgtggac
     1381 ctgcgatccg tcgaatcgca ctcgctccgc cgagcggttg gtgtagttcc tcaggatccc
     1441 ttcctcttca cggggagcat ccgcgacaac atcgtctacg gaaggcccga tgcgacggat
     1501 gccgaggttt acgaagcggc tcggctagtc ggtgcccatg acatcatctc cacctcgctc
     1561 aagggctacg acacgcacat caaggggcgt ggtcgcgggc tatcggccgg tgagaaacag
     1621 ctcatcgcgc tggctcgcgt tgctctggtc gatccgaaga tcctgctcct cgacgagccg
     1681 acctctcgtc tcgacctaca gaccgaagct cagatcctca ccgcgctcga ccggctgctc
     1741 gatggacgca cgagtctgat catcgcgcac cgactctcga ccatccgcaa cgcggaccgc
     1801 atcgtcgttc tggacgaggg tcggatcgtc gagcagggtc cgcacgcgga gctgcttgat
     1861 cggcgaggcc tctacagcga gatgcacgag aggtggctcg gcgaggctcc cgaacccgtg
     1921 gcccaggtca gctgacgcgc ttgcgctgaa ccacccacgc ggtcacagca agggctccgg
     1981 cggcgtaggc gatcgcccat acggtgagac cgagggtgtt gcgcgggagc gtgatgatcc
     2041 cagcgatctg ttggttcgat aggggagagg tcaggaactt gggagtgaca taccaggcga
     2101 ggttcaggat gtccgcaagg aaacccttga gtagtccgcc ggtgaccagc ccccggatac
     2161 tcgccaccgc acccgcacca gcctgaaaga tgtaagcggc gatcgcggcg agcgccgctg
     2221 gattcaccag ggcgctcaac atcgtggtga tggctccgac gaccaccagg ttgaacgcga
     2281 ccgtgcccca gtgttcccac agcagcgcga atcgatgctg gcccgtgaga agaccgggca
     2341 gtgaagtctc gacggccaag agtgcgcaga ggcccactgt gatcgaggag ctagctatga
     2401 aacgtcccat cgcatatctc gctcttccga gcggccgggc caggatcatc gcggcttcac
     2461 cacttgcgaa cccgggcccc acgttgttca tcgagacggc cagagtggcg atcagcgcga
     2521 aggtctgcat gatccccaga gatgcgatgg tagcgaggtc gatccgaccc gtcgcgttga
     2581 gtgcccccag ttcgttgccg ttgagtgcca gatagatgag tcccgctgtt ggcaacagcg
     2641 atccgagcac gaagaaggct atgagttt
//
LOCUS       DATDOZ010000097         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1903791, whole genome shotgun sequence.
ACCESSION   DATDOZ010000097 DATDOZ010000000
VERSION     DATDOZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903791"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..522)
                     /gene="moaC"
                     /locus_tag="VI541_01855"
     CDS             complement(37..522)
                     /gene="moaC"
                     /locus_tag="VI541_01855"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HLF69680.1"
                     /translation="MTDSGITSHLDESGSIHMVDVSTKDTTVRVASATAFVRMSPDGL
                     ELLREGRLAKGDALTTAKLAGIMAAKKTHDLIPLCHPLSLSSIDVVCEIADSGVKVTT
                     EVRTVERTGVEMEALVAASIAALTIYDMCKSVDRAMVVEEVMLLSKSGGRSGAWRRPT
                     D"
     gene            complement(519..1382)
                     /locus_tag="VI541_01860"
     CDS             complement(519..1382)
                     /locus_tag="VI541_01860"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HLF69681.1"
                     /translation="MPPRKAVIPAAGLGTRFLPASKAVPKELITLVDRPVIQYAVEEL
                     ARAGITNICIVNSHGKEAVADHFTTAPRLEAALIKAAKDELLRDLKRISNLAEVYSVR
                     QNEPLGLGHAVWVAREHIGEEPFVVSLPDEIFDPRDNFLSEMISGFDETGCSIVAVHE
                     VSHEEIQLYGAIDPVDADAEIIEVRGVVEKPHPSVAPSHLALSGRYVLGPEVFDIIEK
                     LEKGAGGEIQLADALGVLAERGTLRAMRYRGRRWDVGKKEGYLEAFVELAAEHPELGP
                     RFRGFLKDFRP"
     gene            complement(1392..>2456)
                     /locus_tag="VI541_01865"
     CDS             complement(1392..>2456)
                     /locus_tag="VI541_01865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HLF69682.1"
                     /translation="AKTLSAHIADLHESREELKRAYTRFGTALRATHDLENLLELVLD
                     TSIDTLDARRGALMLLGPRGSLRVAVSRGIDDTFDLEVGKSVAGYVVKSGEALRYPSE
                     GESEGQMLPPAGSGYDSMLAVPLYSESRIIGVLNLYERENSSPYGQTDLATLVSLADQ
                     AGVAVENVLLHREAQRLAIMDGLTGIWNHRYFQMQFDQEIDRAARFGHPFALVILDID
                     DFKEVNDTYGHQFGNTVLVGLASRVKSVIRDIDRFARYGGEEFVLILPETDAQGAVMT
                     AERVRSAIASKPFQGETVVATVDVSVSVGIACYPEHGFDKATLIRAADSAMYQAKTKG
                     KDRVVLFEGGTPAMPQSAPE"
BASE COUNT          477 a          784 c          700 g          495 t
ORIGIN      
        1 acttcaagcg ccccaagccg caggacccgg agtagttcag tcggtaggac gcctccacgc
       61 accactgcgc ccacccgact tcgagagcag catcacctct tcgacgacca tggcccggtc
      121 gaccgacttg cacatgtcgt agatcgtgag cgctgcgatc gaagccgcca cgagagcctc
      181 catctcgacc cccgtccttt cgaccgtgcg aacttcggtt gtgaccttca cgccggaatc
      241 tgctatctcg cacacaacat cgatcgaaga cagtgagagc ggatgacaca gaggtatcag
      301 gtcgtgggtc ttcttagcgg ccatgatgcc cgcgagtttc gcggttgtca gcgcatcgcc
      361 cttcgcgagg cgtccctcgc gcagcagttc gagcccatcg ggggacatcc tcacgaatgc
      421 ggtcgcgctg gcgacacgaa cggtcgtgtc tttcgtcgac acgtcgacca tgtggatcga
      481 cccagattcg tccagatggg aagtgatccc cgagtcggtc atggtctgaa gtccttcagg
      541 aatccgcgga accgggggcc gagttcagga tgttccgcgg ccaactctac gaaggcttcg
      601 agataacctt ccttcttccc gacatcccaa cgacgaccgc ggtagcgcat cgcccgcagg
      661 gtcccccgct cagcaaggac gcccaacgca tcggccagtt ggatctctcc cccggccccc
      721 ttctcgagtt tctcgatgat gtcgaaaact tcaggcccca ggacatacct cccactgagt
      781 gccagatgtg atggtgctac cgaagggtgc ggtttctcga cgacgccacg gacctcgatg
      841 atctcggcat ctgcgtctac cggatcgatg gctccataca gttggatctc ctcgtgcgat
      901 acctcatgta ccgcaacgat gctgcacccg gtctcatcga accctgagat catctcgctc
      961 aggaagttgt cgcgcggatc gaagatctca tcaggaagcg agacgacgaa tggctcctcg
     1021 ccgatgtgtt cccgcgccac ccagaccgcg tgaccaagac ctagaggctc gttctgcctc
     1081 accgagtaga cctcggccag gttcgagatc cgtttcaggt cccgcagcag ttcgtccttt
     1141 gcagctttga tcaacgcagc ctcgaggcgc ggggccgtgg tgaagtggtc ggccactgcc
     1201 tccttgccat gcgagttgac gatgcagatg ttcgtgatcc ctgccctcgc caactcttcc
     1261 accgcatact ggatgaccgg tctgtcgacg agtgtgatga gctccttggg cacggctttc
     1321 gacgctggca agaaacgagt gccgaggcca gctgcgggga tgaccgcctt acgtggaggc
     1381 atattcgaat cttactcggg ggctgattgg ggcatcgcgg gcgtgcctcc ttcgaagagt
     1441 acgacgcgat ccttgccctt ggttttggcc tggtacatcg ctgagtctgc agcacgtatg
     1501 agcgtcgcct tgtcgaagcc atgttcggga tagcaagcga tgcccacaga cacgctaacg
     1561 tcaaccgttg cgacgacagt ctcgccctgg aacggtttcg aagcgatcgc cgaccgaacc
     1621 ctctcggcgg tcatcactgc cccctgagca tcagtttccg gcaggatcaa cacgaactcc
     1681 tccccgccgt atcgtgcgaa ccggtcgatg tcgcggatga cggatttcac tcggctcgcc
     1741 aatccgacca gaaccgtgtt gccgaactgg tgtccgtagg tgtcgttgac ctccttgaag
     1801 tcatcgatgt ctaggatcac cagcgcgaag gggtgtccga agcgcgccgc tcggtcgatc
     1861 tcctgatcga actgcatctg gaagtaccgg tgattccaga tcccggtgag tccgtccatg
     1921 atggcgagac gttgtgcctc gcggtgaagc agcacattct ccacagcaac cccggcctga
     1981 tcggctagag agaccagggt cgccaggtcg gtctggccgt agggcgaaga gttctcgcgt
     2041 tcgtagagat tcagcactcc gatgatccgc gactccgaat agagcggaac cgcaagcatc
     2101 gagtcgtaac cgctgccagc agggggcagc atctggccct cactctcacc ttctgacggg
     2161 taacgcagcg cctcacccga cttcacgaca tacccggcca cactctttcc cacttcgagg
     2221 tcgaaggtgt catcgatacc tcgcgaaact gcaacacgaa gggagcctcg ggggcccagg
     2281 agcattagtg ctcccctgcg cgcgtcgagc gtatcgatac tcgtgtcgag gacgagttcc
     2341 agaagattct cgaggtcatg ggtcgcccgc agagccgtac cgaagcgcgt gtaggcccgc
     2401 ttcaactcct ctcgtgactc gtgaagatcg gctatatgtg ccgagagcgt cttagc
//
LOCUS       DATDOZ010000098         3541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_341950, whole genome shotgun sequence.
ACCESSION   DATDOZ010000098 DATDOZ010000000
VERSION     DATDOZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341950"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VI541_01870"
     CDS             <1..282
                     /locus_tag="VI541_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69683.1"
                     /translation="GMVLPLSWTPTSASRLFPTMQGEILLEGLGPDLSQITFRGTYRP
                     PFKDIGSSLDRTGLHRVAEATVKHFVDRLRTALLEHDLRDIAVNRAKIS"
     gene            complement(283..2526)
                     /locus_tag="VI541_01875"
     CDS             complement(283..2526)
                     /locus_tag="VI541_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69684.1"
                     /translation="MGTGDSAEARRSSDGYDMLLEFREEVIQDLLIRHLTSSRSGVER
                     EIVSGALRIWWDEPQFSIHPRSITLSIEIRGGLTTAAGRNLSLSGSVEAKMDPSLGYF
                     AGTPCLRLTTKSVDLSDVEVSYAGEPTGREQAPDAFTPVGTIASAIRSHLLDKIAAVP
                     MSYGITSFHVAARDGSGGTAVPPISPEALEPYSVAAVGASIDVRGPEPDDAVAIWVTF
                     DPGVHSEGPVPTVLGPGNRAGVALTFSTSGLNDIIEQLGRAGVLPSLAWNEQRKSWAR
                     FTWMHLECLDGSLRIKGQVGCGGESGPLSGELELDASRGGDISIKVTSTECPFAQEKD
                     LEQFFGGFLRILLRSGHSGTLPQRFEAPGSEVQVEAEPMDVEVAPGRVTFFYDLDETD
                     VKFTKASPSRQPGVVLDQDSVPQQARPGTSVTAFIQARVAGESFPPYDYVWQTADSKR
                     RLIGRGPRLDVVGTPKGSLGMGDVELTRASVTLIDAAGQLSSASRTVVARSARKRVRM
                     TGGSRSRFRVFRRAVGYSFLLVTLAGLGLGYAWVAAFFEETRPPSKEVISPRVLAEKL
                     TSDVSPAASPLPSPSAPTSQAPVRKQRRLRVIASGSPSPSPSPSASPSPSPSGPAQAL
                     VAPAPSFFISPASRSDVCSSDFTLDPFDVLLENPTASNGTWSASFVQPGSFLSDPWGS
                     ISPSTSGVIFAGGSHKVTVTPDEGLCANQAQSDASSSTYQLDFHFVPDDGSGTVTRAF
                     LYTVEIA"
     gene            2701..3444
                     /locus_tag="VI541_01880"
     CDS             2701..3444
                     /locus_tag="VI541_01880"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HLF69685.1"
                     /translation="MTVWDERRKEPQVDLGDQTQLEAYARDLRVSFAAEKHRSHELAQ
                     ALADIKQNYLATVRALAIAVEAKDAYTAGHLRRVTSLGLLMLKQLDPELHSNPQFEYG
                     FLLHDIGKMGVPDSILGKPGPLTEAEWKVMRSHPIIGRRILDEIPFLVEAKQIVLSHH
                     ERWDGGGYPYGLAGEEIPLGARLFSVADTFDAMTTDRPYRKATSIREAVEEIKRGSGT
                     QFWPKAVEAFMSLPHHDLLSESSETAGSS"
BASE COUNT          746 a         1107 c         1044 g          644 t
ORIGIN      
        1 gggatggttc taccgctctc gtggacgccg accagcgctt cgaggctctt tccgacgatg
       61 cagggagaga tactcctcga gggtctgggc cccgacctaa gtcagatcac tttccgtgga
      121 acctatcgtc cgcccttcaa ggacatcggt tcgtccctcg atcggactgg tctgcatcgc
      181 gtcgctgagg cgacggtcaa gcacttcgtc gaccgcctgc gcactgcgct cctcgagcac
      241 gacctaaggg acatcgcggt caatcgcgcc aagatctctt agtcaagcga tctcgaccgt
      301 gtagaggaaa gcacgcgtga cggttccgga tccatcgtcc gggacaaaat ggaagtcgag
      361 ctggtacgtg gagctgcttg catcactctg ggcttggttg gcgcagagac cctcatcggg
      421 agtgacggtc accttgtgac taccgccagc gaaaatgacc ccagaagtcg aaggggagat
      481 gctcccccat ggatcagaca ggaacgaacc tggctgaacg aagcttgcgc tccatgttcc
      541 attggaggca gtgggattct ccagcaacac atcgaatggg tccaaagtga aatccgagga
      601 acagacatca gagcgcgatg ccggagagat gaagaaggac ggcgcaggag cgacgagagc
      661 ctgagctggt ccgcttgggc tcggtgatgg actcgcggac ggactggggg atggactcgg
      721 agacccggat gcgatcaccc tgagtcgcct ttgcttgcgc acgggggcct gactcgttgg
      781 cgcgctcgga gagggtaggg ggctcgcagc aggagatacg tcgctggtca gcttctccgc
      841 caggaccctc ggagagatca cctccttcga agggggtctg gtctcctcaa agaacgcggc
      901 gacccatgca tagccaagcc ccagccctgc cagcgttacc aggaggaagc tatagcccac
      961 cgctcttctg aatactctga acctcgagcg agaacctccc gtcatccgaa cgcgtttgcg
     1021 agccgatcgc gcgacgacag tcctcgaagc tgaactcagc tgtccggcag catcgatcaa
     1081 tgtgactgac gcgcgagtca gctcaacatc tcccatacca agtgagccct tgggtgtgcc
     1141 caccacgtct aggcggggcc cgcgcccgat gagacgtcgc ttcgagtctg cggtctgcca
     1201 aacgtagtcg tatggaggga acgattcacc agccaccctg gcctggatga atgcggtcac
     1261 cgaggtcccc ggccgcgcct gctgtggcac cgagtcctga tcgagaacca cccccggctg
     1321 ccgagacggg gacgccttgg tgaacttcac atcggtctcg tcgagatcgt agaagaaggt
     1381 aacgcgcccg ggggcaacct ccacatccat gggctcggcc tcgacctgca cctcagaccc
     1441 gggggcctcg aacctctgcg gcagagtacc gctgtgaccg gagcgcagaa ggatgcggag
     1501 gaaccctccg aagaactgct ccaggtcctt ctcctgcgcg aagggacatt cggttgacgt
     1561 gaccttgata gagatgtcgc caccgcgtga cgcgtcgagt tcgagctctc cggagagagg
     1621 tccgctctcg cccccgcaac caacctgacc ctttatgcgg agcgacccgt cgagacactc
     1681 gagatgcatc caagtgaatc tcgcccagga cttacgctgc tcgttccatg cgagtgaagg
     1741 caatacaccc gcccttccca actgctcgat gatgtcattc aggccgctgg tgctgaaagt
     1801 cagcgcgaca ccagcacgat tcccgggacc aagaacggtc ggcaccgggc cctctgagtg
     1861 gacgccaggg tcgaatgtca cccatatcgc aacagcgtcg tccggctccg gcccccgcac
     1921 gtcgatcgac gcgccgacgg cggccactga gtatggctcc agggcctcag gtgagatcgg
     1981 gggcaccgct gtcccgcccg atccatcacg cgcggcaacg tggaacgatg tgatgccata
     2041 tgacatcggc acagctgcga tcttgtcgag aagatgtgaa cggatcgccg atgcgatcgt
     2101 cccgaccgga gtgaaggcgt cgggagcctg ctcgcgaccg gttggctcgc cggcatagga
     2161 gacctcaacg tcggagagat cgaccgactt tgttgtgagc cgaagacatg gggtccccgc
     2221 gaagtaaccc aaggatggat ccatcttggc ttcaacactt ccggagagtg acaggttgcg
     2281 tcccgccgcg gtagtcaggc ctccgcggat ctcgatcgat agcgttatcg acctgggatg
     2341 aatcgagaac tgcggctcat cccaccagat ccgcagggcc cccgaaacga tctcgcgctc
     2401 gacccctgac cgagatgaag taagatgtcg gatgagaagg tcctggataa cctcctctcg
     2461 gaactcgagc agcatgtcgt acccatcgct cgaccgacga gcctccgcac tgtctccggt
     2521 gcccaagata tcccccctcg acgatgaatc cggtccacga cctcgctggt cgcatcgaac
     2581 cggttcacaa taagtgtaag taagagcccc cctagccaac aagaggagac ttcaggagaa
     2641 cccacttctc gaccgatgta gttggagccc atgttccgtt acctaggggt ttgataacaa
     2701 gtgaccgttt gggatgagcg acggaaggaa cctcaagtcg atctgggaga ccagacccag
     2761 ctggaagctt acgcgcggga tctgcgcgtt agcttcgcgg ccgagaagca tcgatcgcac
     2821 gagcttgctc aggccctcgc cgatatcaag cagaactacc tggcgaccgt ccgcgcactt
     2881 gcgatcgcgg tagaggccaa ggacgcctac accgctggac accttcggcg agtgacgtct
     2941 ctgggactgc tcatgttgaa gcagctggat cctgaacttc actccaaccc acagttcgag
     3001 tacggcttcc ttctacacga catcggcaag atgggggttc ccgactccat actcggcaag
     3061 ccaggccccc ttaccgaagc cgagtggaaa gtcatgcggt cgcacccgat catcgggcgg
     3121 cgcatcctcg atgagatccc gttcctcgtc gaagcgaaac agatagtcct ctcacatcac
     3181 gaacgctggg atgggggtgg ctatccatat ggcctcgccg gtgaagagat accgctcggc
     3241 gcgcgcctgt tctctgtcgc cgacacattc gatgccatga caaccgacag gccgtacaga
     3301 aaggcgactt cgatacgaga ggccgtggaa gagatcaagc gtggttcggg cactcagttc
     3361 tggcccaaag ccgtcgaggc tttcatgtcg ctcccccacc acgatcttct ttcggagtca
     3421 tccgaaacag cgggttcgtc atgaccccag caagatccaa cacgatcctt gccctgatac
     3481 tcactatcgc ggcttgcctg ctgctcgggg gcctgaccga gtacctcatc ctgagtgcga
     3541 t
//
LOCUS       DATDOZ010000099         4008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3227651, whole genome shotgun sequence.
ACCESSION   DATDOZ010000099 DATDOZ010000000
VERSION     DATDOZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3227651"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VI541_01885"
     CDS             complement(<1..114)
                     /locus_tag="VI541_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF69686.1"
                     /translation="MRILVTGAAGFVGRHLVNELRHAGHEVEATSLNGRDAT"
     gene            complement(111..551)
                     /locus_tag="VI541_01890"
     CDS             complement(111..551)
                     /locus_tag="VI541_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLF69687.1"
                     /translation="MERDRKMLRTAAVALLSVLLATGGQLLLKSGMTQVGYIGKEKLG
                     SPVKLVLHVLTTPQVLLGLLLFVFSAAVWLVVLSRVPLSFAYPFVGITYVLTTLFARF
                     VLHEHVPSLRWVGLALIVSGILIIGRTAPDVDSGPAASSMSTPD"
     gene            complement(562..2655)
                     /locus_tag="VI541_01895"
     CDS             complement(562..2655)
                     /locus_tag="VI541_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLF69688.1"
                     /translation="MRAWILERFQVYARVSLLLAVFLSPIVFSRLRSQDVFNLLKFTS
                     LWVFGLGALALMIIWSAERRRWFPRFNILVAAAAFIVACVVATLLSENPALSLVGLYH
                     RYGGLLPFTLYALILTTIVGLYWEKPLDLRDIAVASAAASVVLLTYVLIQAVGLDWIP
                     WKDSTGKSPPFPVGTMGNSNFAGGYLGIAIPLLLYVALSAKEYYVKMGVGVLVGLDAL
                     ALWFTQTRGGFIAAGVGLLVLAFLNRHRLPRWAQIAALVAVGLATIVAVLVLWHPGSD
                     KPPGPLARVEALRTGTFEVRTFYWTTAFKIFLKHPVAGVGLDLYYANYPLHRLPEDGA
                     QLGLTITDKPHNIYLEYMANAGILGIGTYLSLIGLALWYAFKRLRHLEGDERLLLAAF
                     VATLAGYLAQGVFSIDVPPLAVMGWICVGAIAVIADPKVSDARRVAAEENLARGRGKV
                     KKGKPPTNRDRTRGKPARWGVHLPVGLATIGLILIGLAPLAADAKARTASSLAADKTR
                     QTEAKDFFLAAARLNPLEATYLSQAANVVEGQAQAEKDPSLSKQHYLTALDLIRRALQ
                     KQPGNIFYVMAIARVNSAMAETDASRFPEAERWWRVATDLDPTDWDVRNRYALLLNAY
                     ANAGGDVSLREKSAVELRKVVTIKPDYQPAWVNLSRVYRALGKNGLAREAMLRAVALS
                     PDDEALKAELNSIPG"
     gene            complement(2652..3656)
                     /locus_tag="VI541_01900"
     CDS             complement(2652..3656)
                     /locus_tag="VI541_01900"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HLF69689.1"
                     /translation="MTLTYIAAFSVALVVALGVTPLARRLALRFGIVDHPNDRKIHTD
                     PVPYLGGLAIVTAFAAAVMGGALVRQVSGGYEEMAAILLGGLVLAGIGLWDDLKVVPG
                     WVKVPIEILLGVSLYFVGARAQLFGFWPLDLLVTVAWVVGVTNAVNYMDNMDGLTAGL
                     VAIASTVFAVLAAASGQFLVASLSAALAGCALGFLWHNRPPARIFMGDAGSLFMGFLL
                     AALGLKLRFDNIARVTFFVPVAVLAVPILDALMVSVSRVRRGLSPIHPGKDHTSHRLV
                     RVGIPSKAAVGMIYFGAAASGWLGVVIAFARPGTAYMLMGWMVVIGLFLAYLLLEVEV
                     "
     gene            complement(3653..>4008)
                     /locus_tag="VI541_01905"
     CDS             complement(3653..>4008)
                     /locus_tag="VI541_01905"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HLF69690.1"
                     /translation="DLIVHGDGSQTRCFGYVLDVIPALIDLMACREAEGLAVNLGSVE
                     EISIRDLAQLVTEVTGSRSEVKLVPYGAVYEAGFEDMPRRVPDISRARKLLGFEPTTT
                     LREIIEAMVEDIRSR"
BASE COUNT          924 a         1229 c         1173 g          682 t
ORIGIN      
        1 cgtcgcatcc ctgccgttca acgatgttgc ctcgacttcg tgtccggcgt gacgcagttc
       61 gttgaccagg tgcctgccaa cgaaaccagc ggctccagta acgaggatcc tcaatctggg
      121 gtcgacatcg aagaggccgc ggggcccgag tcgacatcgg gcgcggttcg cccgatgatc
      181 aggataccgg acacgatcag ggccaacccg acccatcgca gtgacggaac atgctcgtgc
      241 agcacgaacc tcgcaaagag agtggtcagc acataggtga tgccgacgaa cgggtaagcg
      301 aaactcaacg gaacacgcga gaggacgact agccagaccg cagccgagaa cacgaagagc
      361 aacagaccga ggaggacctg cggagtcgtg agcacgtgga ggactagctt caccgggctt
      421 ccgagcttct ccttgccgat gtaacccacc tgagtcatgc ctgacttgag gagcaactgg
      481 cccccggtag ccaacaacac cgagagaagt gcaaccgccg ccgtacgaag catctttctg
      541 tccctttcca tcgtcttgtc cttagcctgg tatcgagttc agttcagctt tgagggcttc
      601 gtcatctgga ctcaatgcca cggccctcag catggcttct cttgcgagac cattcttacc
      661 gagggctcga tacactcggc tcaggttcac ccacgctggc tgatagtcgg gcttgatcgt
      721 gacgaccttg cggagttcca cagcggactt ctcacgcaga gaaacatctc ctcccgcgtt
      781 ggcataggcg ttgagcaaca acgcatatcg attccgcaca tcccaatccg tcggatcgag
      841 atccgttgcc acgcgccacc agcgctcagc ctcggggaat ctggatgcat cggtctctgc
      901 catggcagag ttcacgcggg ctatggccat gacatagaag atgttccccg gttgcttctg
      961 cagggccctt cgaatgaggt caagtgccgt gagatagtgc tgcttgctca gagaggggtc
     1021 cttctccgct tgagcctgtc cctcgacaac attggcagcc tgagaaagat aggtagcctc
     1081 caaaggattg agtctggctg ctgcaaggaa gaagtccttg gcttcggttt ggcgagtctt
     1141 atcggcagcc agtgatgatg ccgtgcgagc cttagcatct gccgccaggg gcgcaagccc
     1201 aatcaggata agacctatcg tcgcaagacc gacgggaagg tgaactcccc accgggcagg
     1261 cttgcccctg gtccggtcac ggttcgtcgg cggctttccc ttcttgacct tgcctcgacc
     1321 gcgcgcgagg ttctcctcag ccgccacccg acgggcatcg ctcaccttcg ggtcggcgat
     1381 cactgctatc gccccgacac atatccaacc catcacggcc aacggcggaa cgtcgatcga
     1441 gaacaccccc tgggccagat atcccgcgag ggtggccacg aaagcggcga gaagaagcct
     1501 ctcgtcgcct tcgagatgtc tcaaacgttt gaacgcgtac cacagtgcta ggccgatgag
     1561 ggataggtaa gttcctatcc cgaggatccc cgcgttagcc atgtactcga gatagatgtt
     1621 gtgaggtttg tccgtgatgg tgagaccgag ctgtgcccca tcctctggca gtcgatgaag
     1681 cggatagttg gcgtaataga gatccaatcc cacaccggca accgggtgct tcaagaagat
     1741 cttgaaggcg gtggtccagt agaaagttcg gacctcgaaa gttccagtgc gcagagcctc
     1801 gaccctcgcg agggggcctg ggggtttatc gctccccgga tgccaaagaa ccaggacagc
     1861 tacgatcgtc gcaaggccca ctgccaccag tgcagcgatc tgggcccatc gagggagtct
     1921 gtgtctgttc aagaacgcga gcaccagcag accaaccccc gctgcgatga aacctcctcg
     1981 ggtctgagtg aaccacaacg cgagggcatc gagcccgacg agtactccga cacccatctt
     2041 cacgtagtac tccttcgcgg aaagagccac ataaagaagc agcgggatcg cgatgccgag
     2101 gtaacctccc gcgaagttcg agttgcccat ggtcccgacc gggaagggag gcgacttccc
     2161 tgttgagtcc ttccacggga tccaatcgag accaacggcc tggatgagaa cgtaggtcag
     2221 aaggaccacc gaggcagccg ccgatgctac tgcgatgtct cgcagatcca ggggcttctc
     2281 ccaatagaga ccaacgatgg ttgtaaggat caaggcgtag agcgtgaagg ggagaaggcc
     2341 cccgtagcgg tggtaaagac caaccagcga aagtgcaggg ttctcagaaa gcagcgtcgc
     2401 tacaacgcat gccacgatga aggctgcggc cgcgaccagg atgttgaagc gagggaacca
     2461 gcgccgacgt tcggcggacc agatgatcat cagggcgagg gccccaagcc cgaagaccca
     2521 gagggaagtg aacttgagca ggttgaagac atcctgagaa cgaagtcggc taaatacgat
     2581 gggtgagagg aagacagcga ggagaagact cactcgcgcg tagacctgga agcgctccag
     2641 gatccaggct ctcacacctc cacctccaac aagagatagg caaggaacaa accgataacc
     2701 accatccaac ccatgagcat gtaggcagta cccggcctgg cgaatgcgat caccacgccg
     2761 agccatccac tcgcagcagc cccgaagtag atcatcccaa ccgctgcttt agagggtatc
     2821 ccgacgcgaa ccaaccggtg agaagtgtga tctttcccag ggtggatcgg cgacagacct
     2881 ctgcgcaccc tcgagaccga gaccatcaac gcgtccaaga tcggaaccgc cagcactgca
     2941 acggggacga agaacgtcac tcgtgcgatg ttgtcgaagc gcagtttcaa gccgagagct
     3001 gcaagaagga accccatgaa gagactgccc gcgtcgccca tgaatatcct ggccggaggc
     3061 cggttgtgcc agaggaatcc aagggcgcac ccagcgaggg cggccgagag actcgctacg
     3121 aggaactgac cagaagctgc ggcgagaacc gcgaagaccg tcgatgcgat cgcgaccaac
     3181 ccagccgtca gaccatccat gttgtccatg tagttcactg cattggtcac accaaccacc
     3241 catgccactg tgaccagaag gtcgagcggc cagaaaccga acagctgggc ccgggctccc
     3301 acgaagtaaa gcgagacccc gagcaggatc tcgatgggaa ccttgaccca tcccggtact
     3361 accttgaggt catcccaaag accgatgccc gccaggacga gcccccccaa caggatcgcg
     3421 gccatctctt cgtagccgcc gctcacctgg cgcaccaagg cccctcccat cactgcggcg
     3481 gcgaatgccg tcacgatcgc gagtccccct agatagggaa cagggtcggt gtggatcttt
     3541 cggtcgttag ggtggtccac tatcccgaat cgaagagcaa gcctgcgggc caggggcgtc
     3601 accccgagag cgacgaccag agccactgag aatgcggcga tgtaggtgag ggtcacctcg
     3661 atcggatgtc ctcgaccatc gcttcgatga tctcgcgaag ggtcgtcgtg ggctcgaacc
     3721 ccaacagctt ccgggccctt gagatgtccg gcacacggcg gggcatgtct tcgaagccag
     3781 cctcgtacac cgcgccgtag ggcacgagtt tcacctcgga gcgcgaccca gtaacctcgg
     3841 tcacaagttg agcaaggtca cggatcgaga tctcttcgac tgagccgagg ttgaccgcaa
     3901 ggccctcagc ctcgcggcag gccatcaggt cgatgagtgc cggtatgacg tcgaggacat
     3961 aaccgaagca cctcgtctgc gagccgtcac catgaacgat caggtccc
//
LOCUS       DATDOZ010000100         4401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3228497, whole genome shotgun sequence.
ACCESSION   DATDOZ010000100 DATDOZ010000000
VERSION     DATDOZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228497"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VI541_01910"
     CDS             complement(<1..428)
                     /locus_tag="VI541_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69691.1"
                     /translation="MRRMSRVIAAAVSVALLSSAPPAFASNPKPINLSVTQSDDADPV
                     TENALVTYALLVNNAGPQPANGIIVNLTLTAGRFVAGGGDRWVCAFNGLTGRCDQRGA
                     NPANEASGQLNVVVQAPSSSTASQITHCATVGTSFTDYQDT"
     gene            597..1184
                     /locus_tag="VI541_01915"
     CDS             597..1184
                     /locus_tag="VI541_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLF69692.1"
                     /translation="MDQPEPLTAELVTPSGESSRDLLLRAATGDPMAARALIDETGPI
                     VYGFVYARVGGVQGVAEDLVQATFLEAMRSAKGYRGEAALSTWLCTIARRQVARHFRS
                     ERRRSVVESRLRVVGAEPDDDLTEELIADSDSIIDALGRIPPLHRQVLVLKYLDDLSV
                     EKIADELGRSRVQVQSLLQRARASLRRAFEESSDE"
     gene            1177..3648
                     /locus_tag="VI541_01920"
     CDS             1177..3648
                     /locus_tag="VI541_01920"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HLF69693.1"
                     /translation="MSDPFGDRIPGIDGPRPFTPEFRDRLITSLVAKPDVSEAGGAEF
                     SSPTKHGDQWSLNIDGPRVLPAALKARLITTLTYGTSRTPTWLVTSVAAACALLLVAS
                     GVVLMSGNQSPQQKIQAFDVDDSLVPTHRQAPIVRPPGSLRGFASSAEFLGYVRGQAL
                     RLAGPYGVRQVSIFGGGQPVAMSRAAAGQTAASQPQPQAMSLPATGGPAFSGTNIQEE
                     GVDEPDIVKTDGRRMVVMSQSKVWILDVSRGASRLQGSIDLPDGAGIFLLGDRLIHIA
                     RTYDSGPPIAEPKPAPRSSPLPPPSPTPETSPESSPEPAITLPVNTSPDPSPSPSPSP
                     SPSPSSSPSAVPITRPPASSFPHHVQVGGRVWTAVNVVDISRPDSPRIDATMRAEGSY
                     VSARLSGGVVRLVLQSGALGPLPVPLRDHSEAGLDAAATANKLAVRRSAVGDWLPRYT
                     LKARGRKSVSGRIVGWDHVSRPEEEAGVSMVTVMTIDPVDPRPDNAISVLGSGEIIYS
                     SLQNLYVTSGRLEDVLTAESGRVPTKEVTRIHKFDISDPSTARYMGTGEVRGHLLNQF
                     SMSEDQGRLRVASTLGFPWNSQSPAESFVTVLKDVGGALTPLGSVGGLGKGERIYSVR
                     FIGPMGYVVTFRQVDPLYVVDLHDPTRPRVAGELKVPGYSGYLHPLSPTVLLGIGRDA
                     NDQGRVGGIRFSLFDVSTPASPRLLYDKTVGAYGYSEVESDHHSFLYWEPREFFAIPA
                     SLSWDGDSPEQFVGLLSVTVSTKSGFGEPTKLTHFGRPRTEDFYPVIRRSLVVGDRFF
                     TVSDVGVLMSDLGSFRDLTWVPLVA"
     gene            3699..4124
                     /locus_tag="VI541_01925"
     CDS             3699..4124
                     /locus_tag="VI541_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLF69694.1"
                     /translation="MPEALVDELVKIDLFADLPPKHLKSIATHVQTRSVREGLEIVRE
                     GAYSGDFFVIFSGSAEVTVRGKHRMTVGPGEFFGEVAILGHTQSSTVKALEHMELGVI
                     DAKEFFALLESEPKVTVHLAQALILRLEELTVRPAGQLA"
     gene            complement(4072..>4401)
                     /locus_tag="VI541_01930"
     CDS             complement(4072..>4401)
                     /locus_tag="VI541_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69695.1"
                     /translation="LSPITKDREYTMTRDFIAERTAAMMDIRNPWWSFIRKLNLPPWA
                     ILIFRLELGIFAVMAQLNATGNWHRIMLEFYGEGEPSSPLGEAESEWLTTRADRRDEP
                     LVPLDEG"
BASE COUNT          805 a         1314 c         1379 g          903 t
ORIGIN      
        1 gtgtcttgat agtccgtgaa tgaagtgcct acggtcgcgc agtgggttat ctgggaagcg
       61 gtactcgatg aaggagcctg gacaaccacg tttaactgac cggatgcctc attggcggga
      121 ttcgctccac gctggtcgca acgacccgta aggccgttga aagcacagac ccatcggtcc
      181 cccccacctg cgacgaatcg tcccgcagtc agggtcaggt taacgatgat gccgttagcg
      241 ggctgggggc ccgcgttgtt cacgagcagc gcataggtga cgagcgcatt ctcggtaacc
      301 ggatcggcgt catccgattg ggttacggag aggttgatcg gcttggggtt gctggcgaag
      361 gcgggtggag cggatgaaag gagagctacg ctcaccgcgg cggcgataac ccgtgacatg
      421 cgacgcatga tggctccttt tctctgagcg atggcccagg gctggtacga accggtcgat
      481 tgtcccatag ggtgtctaat cacgacaaga cggccgcaga tacttttccg tttgtgccga
      541 gactgtttgg gcagggccgc ggttgaaagc actgcagagg ggccactaac agggaaatgg
      601 atcaacccga acccttgacc gccgaactcg tcaccccttc tggggagagc tctcgagacc
      661 tgcttcttcg ggcagcaacg ggtgatccga tggctgcccg agcgttgatc gacgagaccg
      721 ggcccatcgt ctatgggttc gtctacgccc gcgtcggcgg ggtccagggc gtggccgagg
      781 acctcgttca agcgacattc ctagaagcga tgcggtcggc aaaggggtac cggggcgagg
      841 cggcactctc gacctggctc tgcaccatcg cgcgtcgcca ggtggcgcgc cacttccgca
      901 gcgagaggcg ccggtccgtc gtcgagagtc gcctgcgtgt tgtgggggcc gagccggatg
      961 acgatctgac cgaggaactc atcgccgata gtgattcgat catcgatgcc ctcggtcgta
     1021 tcccgccgct ccacagacag gtcttggtat tgaagtatct cgatgacctt tccgtagaga
     1081 agatcgccga cgagctaggc cggtcgcgcg ttcaggtcca gtctttattg cagagagctc
     1141 gtgcaagtct aagaagggcc ttcgaggagt cttccgatga gtgatccgtt cggcgatcgc
     1201 atccctggca tcgatggccc gcgtcccttt acccccgagt ttcgtgatcg tttgatcacg
     1261 agccttgtcg cgaagccgga tgtaagtgag gccggggggg ccgagttcag ctcaccgacg
     1321 aagcatggcg accagtggtc cttgaatatc gatggccctc gagtcctgcc cgctgctctg
     1381 aaggcgcgcc tgatcacgac gctcacctat ggcaccagtc gtacccccac ctggctcgtc
     1441 accagcgtcg ccgcagcctg cgcgctgctt ctcgttgcga gcggtgtggt cctcatgtcg
     1501 gggaatcagt cgcctcagca gaagatccaa gctttcgatg tcgacgactc ccttgtccca
     1561 acccacaggc aggcccccat cgtgaggcca ccgggttcgc tgagagggtt cgcatcgtcc
     1621 gcagagttcc tcgggtacgt tcgcggccag gcacttcgcc ttgccggacc ctacggtgtg
     1681 cgccaagtga gcatcttcgg agggggccag cctgtcgcga tgagcagggc tgctgcaggg
     1741 cagactgccg cgtcacagcc ccaacctcag gcgatgtcac tgcctgcgac cggtgggccc
     1801 gctttttcgg gtaccaatat ccaggaggag ggcgtcgatg agcccgacat cgttaagacc
     1861 gatggccgcc gcatggtggt tatgagccaa tcgaaggtgt ggatcctcga tgtgtcacgt
     1921 ggagcctcga gacttcaagg atcgatcgat ctgcccgatg gcgcggggat attccttctc
     1981 ggagaccgcc tgatccatat agctaggacg tacgactccg ggcccccgat cgcggagcca
     2041 aagcccgcgc cgcgttcttc accgctgcca ccgcccagtc caactcccga aacttctccg
     2101 gagtcaagcc cggaaccagc catcacgtta ccggtgaaca cgtcgccaga tccgtcgccg
     2161 tcgccgtcgc cgtcgccgtc gccgtcgccg tcgtcgtcac catcggcagt gcccataact
     2221 cgaccgccag cgtcgtcctt cccgcatcac gttcaagttg ggggacgcgt ctggaccgcg
     2281 gtcaacgtgg ttgatatctc gcggcccgac tctcctcgga tcgacgcaac catgagggca
     2341 gagggctcct atgtatctgc tcggctatcg ggaggggttg tgcgattggt ccttcagtcg
     2401 ggcgcgctgg gccccctacc tgtgccccta cgtgaccact ccgaagcggg cctagacgct
     2461 gcggcgacgg ccaacaagct ggctgtccga cgttcagcgg tcggggactg gcttccgcga
     2521 tacacactca aggcgcgtgg gcgcaagagc gtatccggac gcatcgtcgg ctgggatcac
     2581 gtgagccgcc cagaggagga agcgggcgtt tcgatggtta ccgtgatgac catcgacccg
     2641 gtcgacccga gacctgacaa cgcgatctcg gtcttggggt cgggtgagat catctactcc
     2701 agccttcaga atctttacgt gacatccggg cgcttggagg acgtcctgac tgcagagagc
     2761 ggcagggttc caacgaagga ggtcactcgg atccacaagt ttgatatctc ggatccctca
     2821 acggcccgct acatgggcac cggcgaggtt cggggccatc ttctgaatca gttctcgatg
     2881 tcggaggatc aggggcgtct aagggtcgca tcgactctcg gctttccatg gaactcgcag
     2941 agtcctgctg agagcttcgt cacggtgctc aaagatgtgg gtggagcgtt gaccccgctg
     3001 ggttccgttg ggggcctcgg aaagggcgag cgcatctact cggtgcgatt catcggcccc
     3061 atgggatatg tggttacgtt ccgccaggtc gatccgctct atgtcgtcga cctacacgac
     3121 cccactcgac ccagggtggc cggggaattg aaggttccgg gttactcggg ctaccttcac
     3181 cccttgagtc caacagtcct gcttggcata ggccgtgatg ctaacgacca gggcagggtt
     3241 ggcgggatcc gcttctcgct cttcgatgta tcaacaccag ccagcccgcg tcttctgtat
     3301 gacaagacag tgggcgccta tggatactcc gaggtggaga gtgatcacca cagcttcctc
     3361 tactgggagc cccgagagtt ctttgccatc cccgcttctc tttcttggga tggcgactca
     3421 ccggaacagt tcgtgggttt gctctcggtc acggtgtcga ccaagagcgg tttcggagaa
     3481 ccaaccaagt tgacacactt cgggcgacca cgaactgaag acttctaccc tgtgatccgt
     3541 aggtcgttgg tggttggcga ccgattcttc acggtatctg atgtgggcgt actgatgagc
     3601 gacctagggt cattccgaga cctgacctgg gtgcccttgg tggcataggc ccccagaacc
     3661 tgctggttgg gtatattgcg aaccaggagg tggtccccgt gccagaagcg ctcgtcgatg
     3721 aactcgtgaa gatagatctg ttcgcagacc tgccacccaa acacttgaag tcgatagcca
     3781 cccacgttca gacacgctcg gtccgcgagg gcctggagat cgtgcgggag ggagcctact
     3841 ctggcgactt cttcgtgatc ttttcgggca gcgcggaggt gactgttcgt ggcaaacacc
     3901 ggatgacggt tggtcccggc gagttctttg gcgaggttgc gatcctgggg cacactcaat
     3961 cttcgacagt caaggctctg gaacatatgg agctgggtgt tatcgatgcg aaggagttct
     4021 tcgccctcct ggagtcggag cccaaggtga cggtacacct ggcccaggcc ctcatccttc
     4081 gtctagagga actaacggtt cgtcccgccg gtcagctcgc gtagtcagcc actccgactc
     4141 cgcctcaccg agcggcgacg atggctcccc ctcgccatag aactcgagca tgatccggtg
     4201 ccagttgccg gtggcattca gttgagccat cacggcgaag atgccgagtt ccagtcgaaa
     4261 tatcaagatg gcccaggggg ggaggttgag tttgcggatg aaggaccacc aagggttacg
     4321 gatgtccatc atcgcggctg tccgttcagc gatgaagtcg cgggtcatcg tgtactcacg
     4381 gtccttcgtg atcggcgaga g
//
LOCUS       DATDOZ010000101         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_594048, whole genome shotgun sequence.
ACCESSION   DATDOZ010000101 DATDOZ010000000
VERSION     DATDOZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594048"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VI541_01935"
     CDS             <1..799
                     /locus_tag="VI541_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF69696.1"
                     /translation="GPDFARYGGHTSCVAIGSGDQGPRLVLDAGTGIRDLTKHLSGPF
                     RGTILLTHLHWDHTQGLPFFAAGDREDAEVEVMLPEQGEDAAKLLAKMMSPPSFPIAP
                     WELRGRWSFATLEDGEHSIEGYSVLALEVPHKGGRTFGYRVSDGRSTIAYIPDHNPIS
                     IGPGPDGFGEYHPDALRLVKDADLVLHDSQYTSEEFAARAHFGHTTAEYAIGLAKHGE
                     ARRIVLFHHDPSRCDDELDRFADIYANPDIEIIVARDGMELEASTPN"
     gene            955..1149
                     /locus_tag="VI541_01940"
     CDS             955..1149
                     /locus_tag="VI541_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HLF69697.1"
                     /translation="MDKYKETLLTPAEVAAMFRVDPKTVTRWAKAGKLSSIRTLGGHR
                     RYRQDEVQGFLNQMRTRHGE"
     gene            complement(1226..2041)
                     /locus_tag="VI541_01945"
     CDS             complement(1226..2041)
                     /locus_tag="VI541_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69698.1"
                     /translation="MPMVPSSFLRIYQPLGSFPEKERAKWAAYIASDPQPPDARRFRH
                     VSFSDYAMTGMLYPATDDEAYIRNVGGHWLVCPWRTRLRVLAGLLSYRNSVPEEAADM
                     WVPEEEALKAASELEALRSEQPEIHSNITTAAWHVPLRWFMMFDDSERTVREVDGKIS
                     ATYEIDVRTARSRVDRGLHILREAGMAESQTQPVEELASWIHEFSYDSLIELDYGGIA
                     QLFTKEELLLDRSATEIWSCLEALALGDYEQSAILYNDLAGWWGRVKALENAN"
     gene            2110..>2508
                     /locus_tag="VI541_01950"
     CDS             2110..>2508
                     /locus_tag="VI541_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HLF69699.1"
                     /translation="MTLFDHPATDGFEQILFNQDKSCGLQAIIAIHSTALGPALGGTR
                     FYPYTSEEAACTDVLRLARGMTYKAAAAGLDLGGGKAVIIGDPKREKTEELFRAYGRF
                     VETLGGRYITAEDVGTAREDMDMVRRETRWA"
BASE COUNT          555 a          725 c          724 g          504 t
ORIGIN      
        1 cgggcccgac ttcgcgcgct atgggggcca cacgtcatgc gtcgccatcg gatccggcga
       61 ccagggcccc aggttggtgc tcgatgctgg caccggcata agggatctca ctaaacatct
      121 tagcggcccg ttcagaggca cgatcctgct tacgcatctg cactgggatc acacccaggg
      181 cctccccttc ttcgccgcgg gcgaccgcga ggatgcagag gtcgaagtca tgctcccgga
      241 gcagggagag gatgccgcca aactgctcgc caagatgatg tcgcctccga gctttccgat
      301 cgctccatgg gagctcaggg ggcgttggtc attcgccacc ctggaagacg gcgaacactc
      361 catcgagggt tactcggtac tcgcccttga ggttccacac aaggggggcc gaactttcgg
      421 ataccgagta tcggatggaa gatcgaccat cgcgtatatc ccagatcaca accccatcag
      481 catcggtccg ggcccagacg gcttcggcga gtaccaccct gatgctttga gactcgtgaa
      541 ggatgccgat cttgtccttc acgattcgca gtacacaagt gaggagttcg ccgcccgagc
      601 ccacttcggg cacacaaccg ctgagtacgc gatcgggctc gccaagcatg gtgaagcgcg
      661 caggatcgtg ctcttccacc acgaccccag ccgatgcgac gacgagctgg atcgtttcgc
      721 cgatatctac gctaacccag atatagagat catcgtggcc cgcgatggga tggaactcga
      781 ggcttcaacc ccaaactgat acggacttaa atttccgatt cgcccgctct gtcacccctc
      841 ggacagttaa atcagcaaga aataggctga tttgcctatt cggttctgcc cgattcttgc
      901 cgattattca tctgttaagg acggaatagg tccgtcggga agggagagag caagatggac
      961 aagtacaagg agactcttct gaccccggcc gaggtggcag ccatgttccg ggtggacccc
     1021 aagacagtaa cccgttgggc taaagcggga aagctgtcat ccatccgaac actcggtggt
     1081 catcgtagat accgccagga tgaggtacag ggattcctca accagatgcg gacccggcac
     1141 ggggagtagc actggctacg aggatcgaac caataacgga cgaccttagg gtcgtccgtt
     1201 tgagtttcag gtcccgatca gtcgatcagt tggcgttctc gagggccttg acgcggcccc
     1261 accagccagc caagtcgttg tagaggatcg cgctctgctc ataatcacct agagcgagag
     1321 cttctaggca ggaccagatc tcggtagcgg acctgtcgag aagcaattct tccttggtga
     1381 agagttgagc gatgcccccg tagtccaact cgatgagcga gtcatacgag aactcgtgta
     1441 tccaactcgc aagttcctcg accggctggg tctggctttc cgccatgcca gcctcgcgaa
     1501 ggatatgaag gcctcgatct actcggctcc ttgcggtcct gacgtcgatc tcataggtag
     1561 ccgatatctt gccatcgacc tcgcggaccg tacgttcgga gtcatcgaac atcatgaacc
     1621 aacgcaaagg cacatgccat gcggcggtcg tgatgttcga gtggatctca ggttgttcgg
     1681 aacgaagagc ttccagttcg cttgcggcct taagggcctc ttcctcggga acccacatgt
     1741 cggcggcttc ttcggggacc gagttgcgat agctgagcag accagcaaga acgcgcagtc
     1801 gcgttcgcca aggacatacg agccaatgac cgcccacatt tcggatgtat gcctcatcat
     1861 ctgtcgccgg gtagagcatc ccggtcatcg cgtaatcgct gaaagaaacg tgacggaacc
     1921 gacgcgcgtc aggtggttgc gggtcggatg cgatgtaggc ggcccactta gcgcgttcct
     1981 tctctgggaa tgatcccagc ggctgataga tgcgtagaaa actcgaaggg accataggca
     2041 taagtgttct attacttcgg ctgaaatcct tctagggaac tatacgtttt tcggcttaaa
     2101 atgcggaaca tgactctctt cgatcatccc gccaccgacg gcttcgagca gatcctgttc
     2161 aatcaggaca aatcctgcgg cctacaagcc atcatcgcta tccactcaac cgcactgggc
     2221 cccgcccttg gtggaacgcg cttctatcct tacacttcgg aagaggcggc atgtacggat
     2281 gtattgcgtc ttgccagggg catgacctac aaagcagccg ccgctgggtt ggatctgggt
     2341 ggcggaaaag cagtgatcat cggcgaccca aagcgcgaaa aaaccgagga actgttccgc
     2401 gcgtacggga ggttcgtcga aactctgggt ggccgataca taaccgcaga ggatgtcggg
     2461 accgcgcgcg aagacatgga catggtgagg cgcgagacac gatgggcg
//
LOCUS       DATDOZ010000102         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2953490, whole genome shotgun sequence.
ACCESSION   DATDOZ010000102 DATDOZ010000000
VERSION     DATDOZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953490"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VI541_01955"
     CDS             <1..311
                     /locus_tag="VI541_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HLF69700.1"
                     /translation="ELASQMSNVQVVVEDLPDEETSGGHEEGGLLLGLYQGVPLTRRS
                     SSNYFGVLPDRITIYRLNIERICASADEVPDAVRRTVIHEVAHHFGISDPRLDELGWG
                     "
     gene            complement(319..1716)
                     /locus_tag="VI541_01960"
     CDS             complement(319..1716)
                     /locus_tag="VI541_01960"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HLF69701.1"
                     /translation="MKRSEVAFLVAAAIATGSIVAARNMTASTTEANVADAGRFLSAG
                     WYCPSPAQDGIDAFMGTANLGADPVHLQRRAIGGGAVSVGKGADLAATRRDVVDIADF
                     GVSSPTGLTEAFGAATTSDELAVGKGVGVALSRCSLQPWDTWYFPTASTAKGSGSNSL
                     LLANPFDEEAVFSARIMTAGGPQSPALLRNIVVPRLTQLRVNLDEAVRETPTFGVEVR
                     VTMGRVIAANMVRFTTRDLHHGLSLDVGVRTPSPSWYLAGGIIPPTGEEFLDLVNPGD
                     REALVAVVFQLSDQQIAPDALQELAVAAGSKVRVRLRDFIPVGSYHGIAVTTLNETRI
                     VAGSQIIDDAPGGGGAENVSGAPEPSERWVVPVGSHLGGDEIAISVANPATTSTKISI
                     TLIDAQGIRTPQELKSIRVDGGRVVLIDLRPYVQGSATAIVRALADGKVAVQSLLRLG
                     PPYQDFASSSGQIFD"
     gene            complement(1713..>2565)
                     /locus_tag="VI541_01965"
     CDS             complement(1713..>2565)
                     /locus_tag="VI541_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69702.1"
                     /translation="SWPRAADTHAAPTSDDSKTISSYLTSTAAQVGSFRVLWLGERWV
                     DPVRPFSRRIDGTPYLITGPQGLTMLDAFEGSPDEGINRLDDSVQALAGRRVHLAGHL
                     LAPASIRFIIVDPNDDGLMSAMRRQRDIALEQQQTHSAIFRNLEWFPRAALIAAGGGK
                     AIPKDPSDVTLMLSNLPSGKDVPASGESDFAAPLPRTRHTRVLLSESFNSGWSASVGS
                     RRLEHGRTLGWINGFDIPPDAKGRLVIRFNRDWIRWLWVLLEALIVTITVTIARSRRR
                     EVTGWLR"
BASE COUNT          487 a          819 c          789 g          470 t
ORIGIN      
        1 aggaactcgc tagccagatg tccaacgtcc aggtggtcgt cgaggacctc cccgacgaag
       61 agacatccgg ggggcacgag gagggcggcc ttctgctcgg tctctaccag ggcgttccac
      121 tgacaagacg ctcctcctcg aactacttcg gggtcctccc agaccgcatc acgatctatc
      181 gactcaacat cgaacgcata tgtgcgtcgg cagacgaggt gcccgacgcg gttcgtcgca
      241 ccgtgataca tgaggtcgcg caccacttcg ggatcagcga cccacggctg gatgagttgg
      301 ggtggggctg agagcccctc agtcgaagat ctgcccagac gacgatgcga agtcttgata
      361 cggggggcct aagcgcagaa ggctctgcac agccaccttg ccgtccgcca gcgcgcggac
      421 gatggcagtc gccgacccct gaacgtaggg ccgcaagtcg atcagcacga cacggccccc
      481 atcaacccga atggatttca gctcttgcgg ggtcctgatg ccttgggcat ctatcagagt
      541 gatgctgatc ttggttgatg ttgttgcagg attggcgact gagatcgcga tctcgtctcc
      601 gccgaggtgc gaaccgaccg ggacgaccca gcgttcgctc ggctcggggg cccccgaaac
      661 gttctcggcc cctcccccgc caggtgcatc atcgatgatc tgagatcctg caactatgcg
      721 ggtttcgttg agagtcgtca cagcgatacc gtggtagctc cctactggga tgaagtcgcg
      781 caggcgcaca cgcaccttcg atccggcagc aaccgcgagc tcctgaagcg catcgggagc
      841 gatctgttga tccgacagct ggaagaccac tgccacgagg gcttctctgt cgcccggatt
      901 gaccaagtct aggaactctt cgcccgtcgg agggatgatg ccgcccgcca gataccagga
      961 aggcgacggg gtcctcaccc ccacatcgag gctcaagcca tgatgcaggt cgcgcgtcgt
     1021 gaatcgcacc atgttggctg cgatgacgcg ccccatagtg acgcgcacct cgactccgaa
     1081 cgtaggagtc tcgcgtacgg cctcatctag attgacccgc agctgcgtga gtctgggcac
     1141 gacgatgttg cgaaggagag ccgggctctg ggggccaccc gctgtcatga tgcgcgctga
     1201 gaacacagcc tcctcgtcga acggattcgc cagaaggagg ctgttcgaac ccgagccctt
     1261 cgcggtgctg gctgtcggga agtaccaggt gtcccacggc tgaagcgagc atctcgagag
     1321 cgcaacgccg acccccttgc cgaccgccag ctcatcgctc gtcgtcgctg cgccgaaggc
     1381 ctcggtgaga ccggtgggcg aggagacgcc gaagtctgcg atatcgacga catcgcgtct
     1441 ggtcgccgcc agatcggcac ccttacccac ggagacggcc cccccaccga tggcccttcg
     1501 ttggaggtga actggatccg caccgaggtt ggcggtaccc atgaacgcat cgatgccatc
     1561 ctgcgctggc gacggacagt accaaccagc agacaggaat ctgcctgcgt ccgcgacgtt
     1621 ggcttcggtc gtcgatgccg tcatgttcct tgcggcaacg atcgaaccgg tggctatcgc
     1681 ggctgcgacc aggaaggcga cctccgagcg cttcatcgga gccaccccgt cacttcccgt
     1741 cgccgcgaac gagcgatggt aaccgtgatc gtcacgatca aggcttcgag caggacccat
     1801 aaccacctga tccaatcccg gttgaatctg atcacgagtc gtcccttggc atcagggggg
     1861 atgtcgaacc cgttgatcca ccccaaggtc cgtccatgct cgagacgtct cgaacccacg
     1921 ctcgcgctcc aacctgagtt gaaggactcc gagagaagca ctcgcgtgtg gcgggtccgc
     1981 ggtaggggtg cggcgaagtc gctctcaccg gatgctggca cgtccttgcc cgaaggcagg
     2041 ttcgataaca tcaatgtcac gtcgcttggg tccttgggga tggccttgcc accaccggcc
     2101 gcgatcagag cggcgcgcgg gaaccactcg aggttcctga agatcgccga atgtgtttgc
     2161 tgctgttcga gcgcgatgtc gcgttgtcta cgcatcgccg acatcaggcc atcgtcgttc
     2221 ggatccacga tgatgaaacg gatggatgct ggcgcgagca gatgccccgc cagatgcacc
     2281 cttcttcctg caagagcctg gacgctgtcg tcgagtctgt tgatcccctc gtccggagaa
     2341 ccttcgaacg cgtccaacat ggtgaggccc tgggggccgg tgatcagata tggcgtgccg
     2401 tcgatgcgtc gtgagaatgg gcggaccgga tcgacccagc gctcccccaa ccacagaact
     2461 ctgaacgatc ccacctgcgc cgcggttgat gtcaggtacg aggagatcgt cttggaatca
     2521 tcgcttgtgg gagccgcatg tgtatccgcc gccctcggcc atgat
//
LOCUS       DATDOZ010000103         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_861233, whole genome shotgun sequence.
ACCESSION   DATDOZ010000103 DATDOZ010000000
VERSION     DATDOZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861233"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VI541_01970"
     CDS             complement(<1..1091)
                     /locus_tag="VI541_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69703.1"
                     /translation="MKRRTTAAARATALLAVLLSTLTANLRAEARVTSINLSSTAVAA
                     APDYASVTYSDPWDYSNKEDQLLDQFAGTLSLSSPRIGLGFLSFETNNGASFDPIRTI
                     GGAEPTGRDSGIHPIDASRFTRLSMRINSNSLENAGVLWFNCPTAVGTCAGGFPFQIK
                     PGWQTYDFDIPAQTRFGSLPLTWSGMLSGLRIIPTNSAGKHFDFDWIRLYQSSPSVTI
                     TWVDNNPGAGADLYWDTDTDPENNSANNPGWGLVQHVASTSASNTTSFVSGAFPPGTY
                     YFYVKDLGDSTTSAVSQGLTIDGIPLPVVDQPDLAGGEDYATAVRGDAWDFSSSSDAA
                     LRNVQSVWYAGGILNATNGPPTQNDPQVQL"
     gene            complement(1088..2011)
                     /locus_tag="VI541_01975"
     CDS             complement(1088..2011)
                     /locus_tag="VI541_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69704.1"
                     /translation="MSMKSPSSQLQGRLARAALLLGILVATSLSATAGETSRFGFEAQ
                     QGARAGAGKGSFVFGLAKGGAARGSARVHNKSPKRLRIRIYGADAIRGVDGSLSVAPF
                     GVPAVGAGRYIRPDVSEVALDVGSDRVVEFSVTRPPSSPGGIGALVAEEIAASSGSGS
                     IDVIERVALIVKVADGSVGPHFSLKGVELSAPLRIFPADATVSAEVTNGTADIIRSTL
                     TTQVTTMTGRKFDLKEVTLDLQPGERREVAMRWESVPRLGGVVSAEVKATWPEGEVKQ
                     ESDRVWIVPIWLLILLILAEGIYLVRRSRRR"
     gene            complement(2022..>2257)
                     /locus_tag="VI541_01980"
     CDS             complement(2022..>2257)
                     /locus_tag="VI541_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69705.1"
                     /translation="DVRDALTSSPVNANPLLVTDVVIAQVEALLTATGTDVSTIVGQS
                     GTYASLPKLVVGDLGGIPSGTYRGVLTVTLIEE"
BASE COUNT          459 a          691 c          650 g          457 t
ORIGIN      
        1 agctgcacct gaggatcgtt ctgagtgggg gggccgttcg tagcgttgag gatcccgccc
       61 gcataccaga cgctctgaac atttctcagc gcagcatcgg aggatgatga gaagtcccaa
      121 gcatccccgc ggaccgcagt ggcgtagtcc tctcctccag ccaagtcggg ctggtcgaca
      181 accggaagag ggatgccatc aatggtcaac ccttgactca ccgccgaggt cgtggagtct
      241 ccgagatcct tcacatagaa gtagtaggtc ccaggcggaa atgcacccga cacgaaactc
      301 gtcgtattgc tagcactggt gctggccaca tgttgaacga ggccccagcc tgggttgttc
      361 gcactgttgt tttcagggtc cgtatcggta tcccaataca gatcagcccc ggcccccgga
      421 ttgttgtcga cccaggtgat cgttacagac ggtgacgatt gatagaggcg gatccagtcg
      481 aagtcgaagt gcttgccagc ggagttggtg gggattatgc gtaatccact gagcatcccg
      541 ctccaggtga gaggaagact tccgaatctc gtctgggctg ggatatcgaa gtcgtatgtc
      601 tgccatccgg gcttgatctg gaatggaaat cctcctgcgc aggtaccgac agccgttggg
      661 cagttgaacc agagcacgcc cgcgttctcc agtgaattgc tgttgatccg catgctcagc
      721 cgggtgaacc gcgacgcatc gatcgggtgg atccctgaat ctcgaccggt gggttctgcc
      781 ccccctatcg tgcgaatagg gtcgaacgag gcgccgttgt tagtctcgaa agagaggaaa
      841 cccaatccga tgcgagggct cgacagacta agcgttccag cgaactggtc gagcaactga
      901 tcttctttgt tcgagtagtc ccatggatcg gagtaggtga ccgatgcgta gtcgggggcc
      961 gcagcgacag ccgtcgatga gaggttgatc gacgtcacgc gggcctctgc tcgcaggttg
     1021 gctgtcaagg tcgagagaag gacggcgaga agcgcggtcg ctctcgctgc ggcggtcgtt
     1081 cgacgcttca cctgcgcctc gaccttcgga ccaggtagat gccttccgcc aagatcagaa
     1141 ggatcagcaa ccagatcggc acgatccaga cccggtccga ctcctgcttc acttctccct
     1201 ctggccatgt ggccttcacc tcagcgctga ctactccccc aagtcgcgga acactctccc
     1261 atcgcattgc tacttcccta cgttcacccg gctgaagatc cagagtcact tccttgagat
     1321 cgaacttcct gccagtcatc gtcgtgacct gcgttgtcag agttgaacgg atgatgtcgg
     1381 ccgtaccgtt tgtcacctcg gcgctcactg ttgcatcggc cggaaagatg cgcaggggtg
     1441 cgctcaactc aacgcccttg agagagaagt gggggcccac ggaaccatct gcaaccttga
     1501 ctatcagtgc gacccgttcg atcacgtcta tggaaccact ccccgacgat gcagcgatct
     1561 cctcggctac gagagcgccg atacctccag gggaagaagg tggtcttgtc actgagaact
     1621 ccacaactcg gtcagaaccg acatcaagcg caacctcgga tacatccggt cggatgtacc
     1681 ttcctgcccc cacggccgga accccgaagg gcgcgacgct gagggatccg tcgacacctc
     1741 ttattgcatc ggccccgtag atgcgaatcc ttagcctctt gggactcttg ttgtgaactc
     1801 gcgccgagcc gcgggccgcg ccacctttgg ccaggccaaa gacgaaagag ccttttcccg
     1861 cacccgctct cgctccttgt tgcgcctcga agccgaaccg cgaggtctcg cctgccgtcg
     1921 ctgagagtga ggttgcaacc aggatcccca acagcaaggc cgccctggct aggcggcctt
     1981 gtagctgact gctaggactc ttcatgctca cctacgatat gtcactcctc gatgagcgtt
     2041 acggtaagaa cgccgcgata tgttccgctc gggatgccac ccaggtcacc cacgacaagc
     2101 ttgggaaggc tcgcgtaggt acccgactga cccactatgg tcgagacatc agtacccgta
     2161 gccgtcagca aggcttccac ctgtgcgatc acaacatccg tcacaagcag agggttggcg
     2221 ttcaccggac tggacgtgag cgcgtcgcgg acatccg
//
LOCUS       DATDOZ010000104         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1136124, whole genome shotgun sequence.
ACCESSION   DATDOZ010000104 DATDOZ010000000
VERSION     DATDOZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136124"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VI541_01985"
     CDS             <1..766
                     /locus_tag="VI541_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69706.1"
                     /translation="LPGEVAAVGFLVSVVALLTQMRAYRHSNEDRTRLEGTLSTVSHE
                     LRTPLTMIQGFAELLLTRKVNDETQTGALTQIHDSSKRVTRIIEGLLSISRIEAGLTE
                     PNLEALDIRPIILEAAATIEADQHLLSVDVDADTPLLGDHDMLLQALTNLVVNAAKYS
                     APGTEVKVEGRRSGSLVSISVSDCGSGIPSKEIPRLFEKHYRATSAVAARIPGTGLGL
                     YITKNLVEMQNGHIRVESKPGVGSTFTISLPVAEQT"
     gene            774..1145
                     /locus_tag="VI541_01990"
     CDS             774..1145
                     /locus_tag="VI541_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF69707.1"
                     /translation="MRKLLIADDEDGIRSLVRMTLESEDYEIIEAKDGQAALQLATEH
                     LPTLALLDVAMPGMTGFAVCRALKENPTTAGITVVMLTARAQDSDRQLGEEAGADDYF
                     IKPFSPLALLRKVDEVFGESG"
     gene            1176..2219
                     /locus_tag="VI541_01995"
     CDS             1176..2219
                     /locus_tag="VI541_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255838022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1515..1517,aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HLF69708.1"
                     /translation="MILRSLQASVAEFESLSGWELTERGACRGDVCIPLTAGTVGDFV
                     DIARLADDLAMPLVHDEKHGIWSLGPTSGGRALTPAVAPPLVLPDIRGGTFELASLLG
                     QKVLLIAWASWUGCRNDLPGWQALRSELNPKGLEIVTVALDTGGPEVCIEYVEAAGAE
                     HPCLIDEAHVVDELFGITNVPNGVWIDEDGVIVRPAEPAWTRTIDYSGVPIPEGAPPD
                     RVELIKLARALRIEGDVYVEALRDWVDKGKESRFALSPDEVVRRSHPRPREVATAAAH
                     FEMGQHLYREGFVDDAIPYFREAHRLQPGNWTYKRQAWSFVDPKQGPTDIYDGDWAAD
                     VRVSGPENYYLVF"
     gene            complement(2363..>2927)
                     /locus_tag="VI541_02000"
     CDS             complement(2363..>2927)
                     /locus_tag="VI541_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLF69709.1"
                     /translation="SVAYNVLEAIVAVAFGMAAGSVALIAFGLDSVAESSSASVLVWR
                     LHAERSGNRSAQDAEKRAVRLVAVAFFILAGYILLKSGYDLLTRSKPDESVPGIVIAV
                     VSILVMPLLAWRKRVAARSLDSRALQADSAQTTLCTYLSVVLLLGLVANALLGWWWAD
                     PISALLIGALALREGRELWRTEDFCCV"
BASE COUNT          606 a          921 c          888 g          512 t
ORIGIN      
        1 tctccccggt gaggtcgctg cggtgggttt cctcgtctcg gtcgtggcac ttcttaccca
       61 gatgagggcg taccgacact ccaacgaaga ccggacccgg ctcgagggaa ccctctcgac
      121 ggtctctcat gaacttcgca ccccgctgac catgatccag gggttcgctg aacttctgct
      181 caccaggaag gtcaacgacg agactcagac gggagcgctc acgcagatcc atgactcctc
      241 gaagagggtc actcgcatca tcgaaggact cctttcgatc tcgcgcatcg aggccgggct
      301 cacagaaccg aacctcgagg ctctcgatat ccgcccgatc atcctcgaag ccgcagcaac
      361 catcgaggct gaccagcacc tgctcagcgt tgatgtcgat gccgataccc cgttgcttgg
      421 cgatcacgac atgctgcttc aggccctcac gaatctcgtt gtcaacgcgg ccaagtactc
      481 ggcccccgga accgaggtca aggtcgaggg caggcggtcc ggttcgctcg ttagcatctc
      541 ggtcagcgat tgcggcagcg ggatccctag caaggagata ccgaggctct tcgagaagca
      601 ctatcgagca acctctgccg tcgctgccag gatcccgggt acgggcctcg gcctctacat
      661 caccaagaat ctcgtcgaga tgcagaacgg tcacatcagg gtcgagagta aacccggggt
      721 cggcagtacc ttcacgatct ctcttccggt tgcggagcag acataatgtc tgcgtgcgca
      781 aactacttat agccgacgac gaagacggga tccgctctct ggttcggatg acgctcgaga
      841 gcgaggacta cgagatcatc gaagcgaagg acggccaagc ggccctacag ctggccaccg
      901 agcatctccc aactctcgcc ctactggatg tcgccatgcc aggcatgact ggattcgccg
      961 tctgccgcgc gttgaaggag aaccccacga cagcggggat aaccgtggtc atgctcaccg
     1021 ccagagctca ggactcggac aggcagttgg gcgaagaagc gggggccgac gactacttca
     1081 tcaaaccctt ctctcccctg gctcttctgc gaaaggtgga cgaagtcttc ggcgagtccg
     1141 gctgaccccg cccaagaatc agctaaggaa ccttcatgat ccttcgatcg ctgcaagcat
     1201 cggtagcgga attcgaatcc ctctcgggat gggagttgac ggagcgcggt gcttgccggg
     1261 gcgatgtttg cattccgctc actgcgggaa cggtcgggga cttcgtcgac atcgctcgct
     1321 tggccgacga tctggcgatg ccgttggtac atgacgagaa gcacgggata tggagcctcg
     1381 gacccacctc ggggggccgg gcgctgaccc ctgcggtggc accgcctttg gttctcccgg
     1441 acatccgagg tgggacattc gaactggcct cgctgctggg acagaaggtc ttgctaatcg
     1501 cctgggcatc ttggtgaggg tgccgcaacg acctgcccgg gtggcaggct ctgagaagcg
     1561 agttgaaccc aaagggcctc gagatagtca cggtggcgct ggataccggg gggccggagg
     1621 tctgtatcga atatgttgag gctgctggag cagagcaccc ctgcctgatc gacgaggccc
     1681 acgtcgtgga cgaactgttc gggatcacca acgtccctaa cggggtctgg atcgacgagg
     1741 acggcgtgat cgtgaggcca gccgagcccg cgtggacgcg cacgatcgac tacagcggag
     1801 tccccatccc cgaaggtgct cctcccgatc gcgtggagtt gataaaactc gcgcgggcct
     1861 tgcgcatcga gggcgacgtt tacgtcgagg cgctgaggga ctgggtcgac aagggcaagg
     1921 agtcgcgatt cgcgttatcc ccagatgagg ttgtccgcag atcacacccg cggccccgag
     1981 aagtggccac cgcggccgct cacttcgaga tgggccaaca cctttaccgc gaagggttcg
     2041 tcgatgacgc catcccttac ttccgcgagg cccaccgcct gcagcccgga aattggacat
     2101 acaagcgaca ggcctggtca ttcgtcgatc ccaaacaggg accgaccgac atctacgacg
     2161 gagattgggc ggcggatgtg agggtttctg gtccggagaa ctactatctc gttttctagc
     2221 cctgagatcg cggccttaag gtcggggcgc caccggaacg gtgtccgatc cactccggat
     2281 ctgccagttc gaatccgacg gcgacgcccg cggccccgac taccactgcc agcggtcctc
     2341 gggctctccc tcagcatggg attcagacac agcagaagtc ttcggttcgc caaagctcac
     2401 ggccttctcg aagagccagc gccccgatga gtagggccga gataggatcc gcccaccacc
     2461 aacccaacaa ggcgttcgct acgagaccta gaagcagaac caccgagagg taggtgcaca
     2521 aagtagtctg tgccgaatcg gcttgaagcg cgcgactgtc gagagaacgc gcggcaacgc
     2581 gctttcgcca tgcgagcagg ggcatgacga ggatcgacac aaccgcgatc acgatgcccg
     2641 gcacgctctc atccggcttc gagcgagtca gcaggtcgta cccgctcttg aggaggatgt
     2701 agccagcaag gatgaagaat gccaccgcca ccaacctgac tgccctcttc tcggcatcct
     2761 gcgcgctgcg gttcccgctc cgctccgcgt gaagccgcca aaccaacacc gaggctgacg
     2821 aggactccgc cacggagtca agaccgaagg cgatcagcgc gacggatcca gcggccatcc
     2881 cgaatgcaac agcgacgata gcttcaagga cgttgtaggc aaccgaa
//
LOCUS       DATDOZ010000105         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1398025, whole genome shotgun sequence.
ACCESSION   DATDOZ010000105 DATDOZ010000000
VERSION     DATDOZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398025"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VI541_02005"
     CDS             <1..1545
                     /locus_tag="VI541_02005"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HLF69710.1"
                     /translation="LLLVDASEGPLPQTRYVLRKALSRGLPVIPIINKIDRPDARIAE
                     VLDEIYALFIDLDAHDDQIDFPVVYTDARAGTATRNIEEPGRDLVPLFQVIVDVTPPP
                     VYDPEMSLQFMVTNLAADSYVGRLAVGRVWNGSLKTGETVAICQRDGVTQTARITKML
                     AHTGLLYEEIDSAGPGDICSIAGLGDITVGDTVADASNPQPLPRLEIDEPTLHMTFGI
                     NTSPLSGREGKYVTSRHLQARLERELLGNVSIVVLPTETPEVFEVRGRGELQLAILLE
                     SMRREGFELEVSKPEVIIREENGKIFEPIEDLVLDLPEQFFGVVSQSLALRKGRMTNM
                     HNHGSGWLRAEFRIPARGLIGFRSEMLTETRGTAIIHSTFAGYEPWAGDVTHRLSGVL
                     VADRLGATTPYALYDLQERGELFVGPGVEVYEGMVIGENARPEDMNANPTKEKKKTNI
                     RTHAADEALRLVPPRMMSLERALEFIEADELVEVTPLNIRLRKRQLDQRLRMRAVQRA
                     KHDRVS"
     gene            complement(1550..>3639)
                     /locus_tag="VI541_02010"
     CDS             complement(1550..>3639)
                     /locus_tag="VI541_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69711.1"
                     /translation="VDPTTDTGRRINFLAAEMPRSTAGKPIDPTEWNRNDGFSPGSMV
                     LTFVPGLDLHRTWGTQNLSVGGPNDPADHIADIGRYSAVDAPILIIDSSTGERWPFMS
                     ELDNNALTGPDERLLIIRPARNFLEGHRYLVGLRNLKDASGALIPAGPQFAAYRDGTF
                     GPLTDPTFERSRRASVDGIINAISGAEAARGNIFTRGDLFLAWDFTIASRDNLSERVL
                     KMRDHAFGLLGDTNLANSVIEGLSPSVNITTPIEISRVTGTVEGTIRVPNYLDRPAKP
                     PVEKDPITGLPQQAAGGRLLYGLDDLPMQNPVVPTIDVEFVCTIPRNASVTNTAHPTI
                     YGHGLLGSRSESKGGSTERDRERNFMPCATNWMGFAEYDIANAVHTLADPSNMPTMAD
                     RSQQGFVNFLYLGRAMAHPGGFAAHPNFKDANGSARINPGELFYDGNSQGGIMGGAYT
                     ALAVDSTRSVLGVTGMNYSTLLNRSVDWEGPLFDEGLIEDPEHWDDHIPSYSSVLYTM
                     FPDKKEQQLIMGILQMLWDRAEADGYAQHMTSDPLPNTPAHQVLMHVAYGDFQVTNFS
                     AEVEARTIGARILDTALLPGRHWATDPFFGLTLFPRDGSNNLLPWNGSALVYWDSGNL
                     LPPNANIPPDEAGGDPHEDPRRDHSGGDQKAHFWLTGNIIDVRSGGAYLTCNPNATSS
                     IPRGPMPVTDWCV"
BASE COUNT          724 a         1097 c         1122 g          696 t
ORIGIN      
        1 ctccttctag tagatgcatc tgaagggccg ctgcctcaga ccaggtatgt ccttagaaag
       61 gccctctccc ggggactgcc ggtcatcccg atcatcaaca agatcgaccg accagacgcc
      121 cgtatcgcag aggtcctgga cgagatctac gccctcttca tcgacctcga tgcccatgat
      181 gaccagatcg actttcctgt cgtatacacc gatgccaggg caggaacggc gacccgcaac
      241 atcgaggagc ccggtcgaga tctcgtgccc ctcttccagg tcatcgtcga cgtcacacct
      301 ccacctgtct acgatcccga gatgtcactt cagttcatgg tgaccaacct agccgccgac
      361 tcctatgtcg gtcggctcgc ggtgggccgc gtctggaatg gttcgctcaa aacgggagag
      421 accgttgcga tctgtcaacg tgacggcgtg actcagaccg cccgtatcac caagatgctt
      481 gcccacacgg ggctcctata cgaagagatc gactccgccg gtcccggaga catctgctcg
      541 atcgcaggtc tgggagacat cactgtcggc gataccgtcg ctgacgcaag caacccgcag
      601 ccactccccc gcctcgagat cgacgaaccc acgctccaca tgaccttcgg gatcaacact
      661 tcgccgctgt cgggtcgaga aggaaagtac gtcacgagcc gtcaccttca ggcccgcctg
      721 gagcgcgaac tcctgggtaa cgtttcgatc gttgttctcc ctacggaaac tcccgaggta
      781 ttcgaggtac gcggccgcgg cgagttgcaa ctcgccatcc tgctcgagtc gatgcgcagg
      841 gaggggttcg agctcgaagt ctccaaaccc gaggtcatca tcagagaaga gaacggcaag
      901 atcttcgagc ccatcgagga cctcgtgctg gatcttcccg aacagttctt cggcgtcgtc
      961 agccagtctc tcgcgctgcg caaaggccgc atgaccaaca tgcacaacca cggcagcggc
     1021 tggttgaggg ccgaatttcg catccccgcc agagggctca tcggttttcg ctccgagatg
     1081 ctgaccgaga cccgcgggac cgcgatcatc cacagcacgt tcgccggata cgaaccctgg
     1141 gcaggcgatg ttactcatcg tctctcaggg gtattggtag cagaccgcct cggtgcaact
     1201 acaccttacg cgctctacga cctgcaggaa cgcggcgagc tgttcgtggg ccccggtgtt
     1261 gaggtctacg agggcatggt catcggcgag aacgcccggc cggaagatat gaacgccaac
     1321 cccacgaaag agaagaagaa gaccaacatc cgcacacacg ctgcagacga ggccctgcgc
     1381 ctcgtgcccc cgcgcatgat gtcgctcgag cgcgcgctcg agttcatcga ggccgatgaa
     1441 cttgtagagg tgacaccact caacatccgc ctacgcaagc ggcaactcga tcagcgacta
     1501 cgtatgcgag cagtccaacg cgcgaagcac gaccgggtca gctagaagct cagacgcacc
     1561 aatcagttac gggcatcgga ccccgcggga tcgatgatgt cgcgttgggg ttgcatgtca
     1621 ggtaggcccc accgctgcgg acatcgatga tgttgccagt cagccagaag tgagccttct
     1681 gatcgcctcc actgtgatca cgccgtggat cttcgtgtgg gtctccgcct gcctcatccg
     1741 gtgggatatt ggcattcggt gggagaagat tgccggaatc ccagtagacg agggccgaac
     1801 cgttccaggg gagcaggttg ttgctgccat cacggggaaa cagcgtaaga ccgaagaagg
     1861 ggtcggttgc ccaatgacga cctggcaaga gtgcagtgtc gagaatgcgg gcaccgatgg
     1921 tccgggcctc cacctcggct gagaagttgg tcacctggaa atccccatag gccacatgca
     1981 tcaggacctg atgagcaggc gtgttaggta gaggatcact ggtcatgtgc tgcgcgtagc
     2041 cgtctgcctc ggcgcgatcc cagagcatct gaaggatgcc catgatcagc tgctgctcct
     2101 tcttgtcggg gaacatggtg tagaggaccg aggagtacga ggggatgtgg tcgtcccaat
     2161 gctccggatc ttcgatcagt ccttcgtcga agaggggccc ctcccagtca accgaacgat
     2221 tgagcagggt cgagtagttc atgccggtca cgccgagcac tgaccgagtc gagtcgacgg
     2281 cgagggcagt gtaggcgccg cccatgatcc cgccctggct gttgccgtcg tagaagagct
     2341 caccaggatt gatccgggca ctgccgttcg catctttgaa gttcggatgt gcagcgaagc
     2401 caccaggatg cgccatggcc ctgcctagat agaggaagtt gacgaagccc tgctgagaac
     2461 gatcagccat cgtcggcatg ttcgacggat ccgcaagcgt gtgcaccgcg ttggcgatgt
     2521 cgtactcggc gaaacccatc cagttggtcg cgcagggcat gaagttacgc tcgcgatcac
     2581 gttcggtcga accgcccttc gactccgagc gactaccgag cagcccgtgc ccgtagatgg
     2641 tcgggtgggc tgtgttcgtg acgcttgcat tcctcgggat cgtgcagacg aactccacat
     2701 cgatggttgg caccacgggg ttctgcatgg gcaggtcatc tagcccatag aggagacggc
     2761 cccctgccgc ctgttggggc agaccggtga tcgggtcctt ctcaacggga ggcttcgcgg
     2821 gacgatcgag gtagttcgga acacggatcg ttccctcgac cgtaccggtc accctggaga
     2881 tctcgatggg ggtcgttatg ttgaccgatg gtgacaggcc ctcgatgacg ctgttagcga
     2941 ggttggtatc accgagtaag ccgaatgcgt ggtcgcgcat cttgaggacc cgctcagaga
     3001 ggttgtctct cgaagcgatc gtgaagtccc aggccaggaa taggtcgcct cttgtgaaga
     3061 tgttgccgcg agcagcctcg gcccccgaga tcgcgttgat gatcccgtcg acgctggcac
     3121 ggcgggaccg ctcgaatgtc gggtccgtca gggggccgaa tgtcccgtcg cggtaagccg
     3181 cgaattgagg ccccgcaggg atcagggcac ctgaggcgtc cttgaggtta cgcagaccga
     3241 cgaggtatcg gtggccttcg aggaagttgc gcgctggtcg gatgatgaga aggcgctcgt
     3301 cggggccagt caacgcgttg ttgtcaagtt ctgacatgaa gggccagcgt tcacccgtgc
     3361 tcgaatcgat gatcaggatg ggggcgtcga ccgccgagta acgtcctatg tcagcgatgt
     3421 gatcggccgg atcattcggc cccccgacag acaggttctg tgttccccat gtccgatgaa
     3481 gatcgagtcc tggaacgaac gtcagcacca tcgatcccgg cgagaagccg tcgttgcgat
     3541 tccactcggt ggggtcgatc ggctttccag cagtgctgcg cggcatctcg gccgccagga
     3601 agttgatgcg ccgccctgtg tcggtggttg ggtccactc
//
LOCUS       DATDOZ010000106         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3477996, whole genome shotgun sequence.
ACCESSION   DATDOZ010000106 DATDOZ010000000
VERSION     DATDOZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477996"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VI541_02015"
     CDS             complement(<1..570)
                     /locus_tag="VI541_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69712.1"
                     /translation="MTEPHSTVGRVLGIEPATSSEFRVVIDVADYLQLDDLVYVTTQV
                     PKAGEVHIYGLVTEVASRYEGARFESDMALIALEGSLPAELSRSASVQIMRVDPEIWV
                     APDAGEAVYRAQGAERDKALYADEMERKLPVGFARDGLPVWVDLDFFDGRKGGHISIS
                     GVSGVATKTSFALAFLRALTAHPKVLGSAA"
     gene            728..1036
                     /locus_tag="VI541_02020"
     CDS             728..1036
                     /locus_tag="VI541_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLF69713.1"
                     /translation="MTVMSKKSFDEPDEVRNPKEDMRVEFVEMGGLRFRRHTASPGYK
                     GQNCDIDHLLFVISGNFHARMPDGQESHFGPGEIAVIPPGHDAWNDGDEPVVWLEIPH
                     "
     gene            complement(1140..2072)
                     /locus_tag="VI541_02025"
     CDS             complement(1140..2072)
                     /locus_tag="VI541_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69714.1"
                     /translation="MAEAQKIWIEAWAPEYGSSFEASDALAATEEEVDTAVEIDEWEA
                     IVPGPTPWRTSAFLDGVSRVDARAFMAAGKVTVPGLCGSVGVGAVIVDGKANYGPLEV
                     HRTAVFGAGASATIPPIDTALDYESRSVQGSRPEELRMGIENLRSEHEDEMARRLAGE
                     GYLVIADGPARIREKLEVVGFIKSHQKTYLNPDLDQVVADLPAGGRTPLFHFGAIRPR
                     YSWYQRLAPRGDQHPWAAIARCEVTAALSLKKAVALADLLTFHLPRFGSKAHWDTRAP
                     QNLIPIAQLERRLRHLLGDRELVYRKIRSALAGG"
     gene            complement(2171..>3452)
                     /locus_tag="VI541_02030"
     CDS             complement(2171..>3452)
                     /locus_tag="VI541_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLF69715.1"
                     /translation="NYGFPLYWTGAIRPLYDVGGGTMAGLLRPLNNPTDTERQKCAGN
                     VATHSRTVGNDPIVQGAVGDTDNDWWRARSLISDIDNVSVPIHITGAYQDEQTGARGP
                     SHLWEKVAGVPKRLVLTNGDHGTNTNTAETRNDRVAWMDHWMRSIDGGFGTLAADEKS
                     VKVLLEMKNSPRASNGSISAADFPIPGTTWTDYNLSTGGSLTTGSPVTGTATYVSGSA
                     RQSYQRLQGDPNSGEEFAQEAGPDELRFATAPLSTDTVVAGPITATLNMSATAPDTEI
                     YVEIDDIGPGGVSHLQRGLLKASHRATTDSLSDHIVGGPHSGDIYRPWRPHTNPQNIL
                     PGTTYKYLVEIWPVGHVFRAGHRIGIKIAAPPLVDGIYAYIPKRAVSLNTIYFGGADP
                     SRIMLPIVPVPTNLSSTAPACGALDSVRCTTEGW"
BASE COUNT          633 a         1101 c         1098 g          620 t
ORIGIN      
        1 cgccgcgctg ccgagcacct tgggatgcgc ggtgagggcc ctcaggaatg cgagcgcgaa
       61 cgatgttttc gtcgcgacgc cggagacccc gctgatcgag atgtgtccgc cctttcgtcc
      121 atcgaagaag tcgaggtcga cccataccgg tagaccatcg cgcgcgaagc cgacggggag
      181 cttgcgctcc atctcatcag cgtagagggc cttgtcgcgc tcggccccct gcgcgcggta
      241 gaccgcctcg cccgcatcgg gcgcgaccca gatctcgggg tcgacgcgca tgatctgcac
      301 tgatgcggag cgcgacaact cagcgggcag cgacccctcc agcgcgatca gcgccatgtc
      361 ggattcgaag cgagcgccct cgtagcgcga ggcgacctcg gtgacgagcc catatatatg
      421 gacctctcca gccttgggga cctgcgtggt cacatagacg aggtcatcca gctggaggta
      481 atccgccacg tcgatgacga cgcggaactc cgacgaggtc gctggctcga tgcccagcac
      541 ccttccgacc gtgctatgcg gttccgtcaa atcgctcccc cccctgacgg gaacaatgct
      601 atcggcagtg tctgacaatc tgcgctccaa tgagatgtca tagcatgatt gcggctcaca
      661 cagtggatgg ggatgatgcg acaacggcct gcaggccgag agagcgccaa caccaaggag
      721 ggtgaggatg acggtgatga gcaagaagag cttcgatgaa ccggacgagg tccgcaaccc
      781 aaaggaggac atgcgggtcg agtttgtgga gatgggcggt ctcaggttcc gccggcatac
      841 ggcgagcccc gggtacaagg ggcagaactg cgatatcgac caccttctct tcgtgatctc
      901 aggcaacttc cacgcccgca tgccggacgg ccaggaatct cacttcggcc cgggggagat
      961 cgctgtcata cccccggggc atgacgcgtg gaacgacgga gacgagccgg tggtctggct
     1021 ggagatcccc cactgacgcg aggccgcagg gcccgaagcg cacggttcca caagccgcgc
     1081 accgcaccgc gacgacgtag gtcggcgggc accctgaatc accggatcgt gcgtggactc
     1141 taaccccccg ccagcgccga gcggatcttc cggtagacga gttcgcggtc cccaagaagg
     1201 tgacggagcc tgcgctccag ctgagcgatg gggatgaggt tctggggggc cctcgtatcc
     1261 cagtgcgcct tcgaaccgaa cctcggcaga tggaacgtga gcagatcagc cagagcgacc
     1321 gccttcttaa gcgagagggc cgcggtcacc tcgcagcgcg caatagcggc ccatggatgc
     1381 tgatctccac gtggggccag acgctgatac caagagtagc gcggccggat cgcgccgaag
     1441 tggaatagcg gagtgcggcc cccggccggg aggtcggcta cgacctgatc gaggtctggg
     1501 ttcagatagg tcttctggtg gctcttgatg aagccgacca cttcgagctt ctcgcggatg
     1561 cgggccgggc catcggcgat cacgaggtac ccctcgcccg ctagccggcg ggccatctca
     1621 tcctcatgtt ccgagcgaag attctcgatg cccatccgca attcttcggg acggctgccc
     1681 tgcaccgagc gagactcgta gtcgagcgcc gtatcgatag gcgggatagt cgccgaggcc
     1741 cccgcaccga agaccgccgt gcgatgaacc tcgagcggac cgtagttggc cttcccatcg
     1801 acgatcaccg cgcccacgcc caccgaaccg cagaggccgg gcacggtcac ctttccggcc
     1861 gccatgaagg cgcgtgcatc gacccgactg accccatcga ggaacgcgct cgttcgccac
     1921 ggtgtgggac ccggtacgat cgcctcccac tcgtcgatct cgacggcggt atcgacctct
     1981 tcttcggtcg cggccagagc atcggacgcc tcgaaggagg acccgtactc gggggcccat
     2041 gcctcgatcc atatcttctg cgcctcggcc atggaggcat ggtatcggca gggtccgaca
     2101 ttttggtcct ttcgcgaata actaccgtag tggtagctat tcgcgaacag agccgtggcc
     2161 cgaggaggct ctaccagcct tcggtcgtac accgcaccga atcgagggcc ccacacgcag
     2221 gtgcagtcga gctgaggttc gtgggcaccg gaacgatcgg cagcatgatc cgagacgggt
     2281 cggccccccc gaagtagatc gtgttgaggc tgaccgcccg cttggggatg taggcataga
     2341 tgccatctac caacggcggc gccgcgatct tgatgccgat cctgtgacct gctcggaaga
     2401 cgtgaccgac gggccatatc tcgaccaggt acttgtaagt cgttccaggc aggatgttct
     2461 gcgggttcgt gtggggcctc catggacggt agatgtcgcc gctgtgcgga ccgccaacga
     2521 tgtgatcgct caggctatcg gttgtcgcgc gatgactcgc cttgaggagg ccccgctgca
     2581 ggtgcgacac gccacccggc ccgatgtcat cgatctcgac atagatctcg gtgtcgggag
     2641 cggttgcgga catattgagg gtcgctgtga tcggcccagc caccaccgtg tcggtcgaca
     2701 aaggggcagt cgcgaatcgt agctcgtcgg ggcccgcttc ctgtgcgaac tcctcgccag
     2761 agttcgggtc accctggagc cgttggtatg actgcctcgc actgcccgag acataggttg
     2821 cagttcccgt cacgggcgaa cctgtcgtca atgagccgcc ggtgctcagg ttgtagtccg
     2881 tccaggtcgt cccggggatc gggaagtcgg ctgcgctgat actgccattc gaagcacgag
     2941 ggctgttctt catctccagc aggaccttga ccgacttctc atcagccgca agtgttccga
     3001 acccaccgtc gatactgcgc atccagtgat ccatccaagc cacgcgatcg ttgcgcgtct
     3061 cggcggtgtt ggtgttcgtg ccatggtcac cgttggtgag aacgagtctc ttcggcaccc
     3121 ccgcgacctt ctcccaaaga tgagatggcc cccgtgcgcc cgtctgctca tcctgatatg
     3181 cgccagtgat atggatcgga acgctgacgt tgtcgatatc gctgatgagg gatcgcgcac
     3241 gccaccagtc gttatcggta tccccgacgg caccctgcac gatggggtcg ttcccgacgg
     3301 tccgactatg cgtagcgaca ttcccagcgc acttctgccg ctcagtgtcc gtggggttgt
     3361 tcagaggtcg aagcagtccg gccatcgttc cgccacccac gtcgtataga ggacggatcg
     3421 caccggtcca gtagagcggg aagccgtagt tg
//
LOCUS       DATDOZ010000107         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2189568, whole genome shotgun sequence.
ACCESSION   DATDOZ010000107 DATDOZ010000000
VERSION     DATDOZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189568"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VI541_02035"
     CDS             <1..484
                     /locus_tag="VI541_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HLF69716.1"
                     /translation="ETPAVKADYGHFVDGDPVTRIVIPKFSVDMIVVEGTGSRSLNTG
                     AGHYPDTPLPGQPGNIGIAGHRTMYGKPFNRIHEFTAGDKVYLITPFDKYTYEILPPF
                     DGHANPWVVEANSWDVVSPTAESMLTMTACHPIGSARQRIVARAKLVATEPLVNATAR
                     "
     gene            481..1530
                     /locus_tag="VI541_02040"
     CDS             481..1530
                     /locus_tag="VI541_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69717.1"
                     /translation="MRLARIITSVALLAAGMSMFASPAQAAIDTTINFRFADPGSTDG
                     TPRAGQQISGAWTVVVEAAAQSSLRSLSVALFADPNEKPVPAPSGTSSESETYPAGDR
                     NSSSISLFWDTAAMTRYNGVYRLVAHADSQLGEEVSTTVEKIYVVNAASAPTGLNAAL
                     NSTSVALNWVDNPEGDILQYEVRRSTDGKRYSEIGQSLSSSYTDPKPPKGVPLTYEVV
                     AVRYSPLDPAGVASAPSQKTSAITIPIPTETRRPAQDLAPVGPADAPTAPKVQAPIIT
                     KNYGSFSSILPYGAPPPQQFVKDGRESSTGRVLAEQRSAGSQPLHTERYIAGAFIMLI
                     GALHLARAARRITAQ"
     gene            1546..2658
                     /gene="thiO"
                     /locus_tag="VI541_02045"
     CDS             1546..2658
                     /gene="thiO"
                     /locus_tag="VI541_02045"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HLF69718.1"
                     /translation="MQDCDVLVIGAGVIGRSIAWRVAQSGASVTLVDRSDADRSSWAA
                     AGMLAPATEARYGENALLDLGLESLRRYPSFLEELSSASGIEVRCGSSGALFVALDRD
                     QAEAAVQLLEFQRSLGLSVRWLEAQECRDYEPALAPSLVGGIRSQTDTEVDPRTLLAA
                     LELASASAGVRTIDDQIASITVSGGRASGVTTMGGVQVSARTIVLAAGCWSGSIENVP
                     NEVKDAIRPVKGQVVRLDSRGPGTTKVCIVRTQDVYVVSRRDHLVVGATSEEKGFNTT
                     PTAEGVYELLRRAIEVLPGIRHHDIIEIGVGLRPGSRDNAPLLGPTSLEGLIAATGHF
                     RNGILLTPVTADAIATLVSSGNVPDTLKVFDPMRFA"
     gene            2655..2855
                     /gene="thiS"
                     /locus_tag="VI541_02050"
     CDS             2655..2855
                     /gene="thiS"
                     /locus_tag="VI541_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017605961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HLF69719.1"
                     /translation="MKLIVNGKETFSDAASIPELLRSLDVTDPRGVAVAVNGEVIARS
                     SWSEVRLNEGERVEVLRAVQGG"
BASE COUNT          576 a          946 c          879 g          526 t
ORIGIN      
        1 cgagacgcct gcggtcaaag cagattatgg gcacttcgtg gatggcgacc cggtcacccg
       61 gatcgtcatc ccgaagttct cggtcgacat gatcgtcgtc gagggcacgg gctctcgctc
      121 actgaacacg ggggccggtc actacccaga caccccactt cccggacagc caggcaatat
      181 cgggatcgca gggcaccgca ccatgtacgg caagccattc aaccggatcc acgagttcac
      241 ggcaggtgac aaggtctacc tcatcactcc gttcgacaag tacacctacg agatcctgcc
      301 gccattcgat ggccacgcga acccttgggt ggtcgaggcg aacagctggg acgttgtgtc
      361 gcccaccgcg gagtcgatgc tgaccatgac tgcttgccat ccgatcggga gtgctcgtca
      421 acggatagtt gccagggcta aacttgttgc aaccgaaccc ctagtgaacg cgacagcacg
      481 atgagactcg caaggatcat cacatcggta gccctcttgg cggcaggcat gtcgatgttc
      541 gcttccccgg ctcaagctgc catagacacg accatcaact tccgattcgc cgatccgggt
      601 tcaaccgatg gcacaccgcg tgcagggcag cagatctcgg gggcctggac agttgtggtc
      661 gaagccgcag cccagtcgtc gctccgctcg ttgagcgttg cgctcttcgc agaccccaac
      721 gaaaagccag tccccgctcc gagcggtaca tcgagcgagt ccgagaccta ccccgccggt
      781 gaccgcaact cctcatcgat ctcactgttt tgggataccg cggccatgac gcgctacaac
      841 ggcgtctacc gactggtcgc ccacgccgac tcacagctcg gcgaagaggt ctcgacaacg
      901 gtcgagaaga tctacgtcgt caatgctgcc tcggcaccaa cggggttgaa cgccgctctg
      961 aactcgacat cggtcgccct caactgggtc gacaaccctg agggggacat cctccagtac
     1021 gaggtccgac gctccacaga cggcaagcgc tactccgaga tcggacaatc actcagctcg
     1081 agctacaccg atccgaagcc cccgaagggt gtgcccctca cctacgaagt cgttgcggtg
     1141 aggtacagcc cgctcgaccc ggctggcgtg gcgtcggctc cctcgcagaa gacctccgct
     1201 atcacgatcc ctatcccaac cgaaacccgc agaccggcgc aggatctcgc gccagtaggg
     1261 ccagccgatg cacctaccgc ccccaaggtg caggctccca tcatcaccaa gaactatggc
     1321 tccttctcat cgatactgcc gtacggcgcg ccgccgcctc agcagttcgt gaaggatgga
     1381 cgtgaatctt ctaccgggag agtcctggcc gagcagcgat ccgcaggatc tcaaccactg
     1441 cacaccgagc ggtacatcgc cggtgcgttc atcatgctga tcggggccct ccatctcgct
     1501 cgggccgcga gaaggatcac cgctcagtag cagtagagtt gagccatgca agattgcgat
     1561 gttctcgtga tcggagcggg tgtcatcggc cgctccatcg catggcgcgt ggcgcagagc
     1621 ggcgcatcgg tgaccctcgt cgatcgcagt gatgccgaca ggtcttcgtg ggctgcagca
     1681 gggatgctgg cccccgctac cgaagcgcgc tatggcgaga acgcactcct tgaccttggt
     1741 ctcgagagtc tgcgccgata cccctccttc ctcgaagaac tctcgtctgc ttcggggatc
     1801 gaagttcggt gcggcagcag cggggccctc ttcgtggcac tggacaggga ccaggccgag
     1861 gcggcagttc aactactcga gtttcaacgc tcactcggac tctccgtgcg ctggctcgaa
     1921 gcgcaagaat gccgagacta cgaacctgcg ctggctccat cgctcgtcgg agggatccga
     1981 tcgcagaccg acaccgaggt cgacccgcgg accctgttgg cagctctcga actagcatcc
     2041 gcgtcggcgg gggttcgcac gatcgatgac caaatagcct cgatcactgt ttccggaggc
     2101 cgagcgagcg gtgtcacgac gatgggagga gtgcaggtgt cggctcgaac catcgttctc
     2161 gccgcaggct gctggtcggg gtccatcgaa aacgtcccca acgaggtgaa ggatgcgatc
     2221 agaccggtca agggtcaggt ggtgcgcctc gactctcggg ggcctggaac cacgaaagtt
     2281 tgcatcgtgc gaacccagga tgtctatgtc gtttcgcgca gggaccatct cgtcgttggg
     2341 gcaacctcgg aggagaaggg gttcaacacc acaccgaccg ccgagggcgt ctatgaactg
     2401 ctccgccgag ctatcgaggt gctgcccggc atccgacacc acgacatcat cgagatcgga
     2461 gtcggtctgc gaccggggag ccgggacaac gctccccttc tcgggccaac ctctctcgaa
     2521 gggttgatag ccgcgaccgg tcacttccgc aacggcatcc tgttgacgcc ggtcacggcc
     2581 gacgccatcg ctaccctagt ctcatcaggc aacgttcctg acacgctcaa ggtattcgac
     2641 ccgatgaggt tcgcttgaaa ctgatagtga acgggaagga gaccttctcc gacgcggcgt
     2701 ccatccccga gcttctacgg tcgctcgatg tcaccgaccc gcgcggggtc gcggttgcag
     2761 tcaacggcga ggtgatagca cgttcatcat ggagtgaggt gcggctgaac gaaggcgagc
     2821 gcgtcgaggt tctgcgagcc gtgcagggag gttgaatatg ccgtccgaga agcttgaggt
     2881 cgcaggtcag gtcgtcgcgt cgcgtcttct cttcgggaca ggcggat
//
LOCUS       DATDOZ010000108         3747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2428749, whole genome shotgun sequence.
ACCESSION   DATDOZ010000108 DATDOZ010000000
VERSION     DATDOZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428749"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..869
                     /locus_tag="VI541_02055"
     CDS             120..869
                     /locus_tag="VI541_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF69720.1"
                     /translation="MERSQSRSQGSALSYPVGPPQARHRSSQISPQIKSTESEARVLV
                     AEPCDIYRRAIVSIIEGGHSGLMLSGEVADRSHVPRAIQITNPDVLVLCLHEMTRFDL
                     IPQAINAVPGIRVVAISYRDDGETVAKAIRAGAMGFLSKRASPEEILFTLKEAARGKV
                     VADPSLLTSDLLWAVAGVDNRRALIESLTDGERKVLDLLAQGMTARAMGVTLFLSKRT
                     IEGRLVSIYRKLGVAGRIEAALLWAHTKEAS"
     gene            866..1408
                     /locus_tag="VI541_02060"
     CDS             866..1408
                     /locus_tag="VI541_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF69721.1"
                     /translation="MNQDLAALNSSNDHGVGLRVVATKDGNGVDEVTPKKPSLKLVRP
                     DDPGPSSTEVVHGQILIIEDNPLISLTLHDALSSIGLKVVSVPTGSAGLAAARSGAIG
                     AIVLDLLLPDMDGMEVLRKLKRDPLTEDVPVVMLTAVGTTGDRKTAMAQGCVAFLLKP
                     PNLAELVNLLDEVSVRTESR"
     gene            1447..3744
                     /locus_tag="VI541_02065"
     CDS             1447..3744
                     /locus_tag="VI541_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69722.1"
                     /translation="MPLRQKIFVPFACLCLMFGAVGTFVYARATVVRERSVRAGQLSS
                     ASAYISLLVKDSALSLAAASRRAVFTEGFAQALADHDEVRLSKLLLPVAVNARHSFYL
                     ALDANGSGAIELAWQGTPEPTPRPAGEWGSEPLFREAVTRSGEYELTPSLLEREGEWF
                     IVVAAPVTVTDGGARGLLVVGDALTPLIRVPKVQNLILGFMDTRGNPVVGSFAARSPG
                     GPAEVLNAVSKVGGKRYETLFSPVQLAGRSIGTLTIAVPAPSGLGMLGGQGFIIGFLA
                     LATLLMAFGVGSFISRSLTRRLAELADAAYEFQSGRRDVRVNADGSDELHEVAAAFNL
                     MATDLQQSHDSLEATIEERTAELKHSLSRLDRANARLTKANEAKSVFLGNVSHELRTP
                     LSGILLASEMLHDRSFKMFSEEKVRELSGKIHGSGRHLLALIDDLLDLSRIEAGRLDL
                     NLQSISLSPLLAEVRSSLGPIASDKGIALDIPSGEGHTVFADPVRMKQVLINLITNAI
                     KFTDVGGRVWAEIVPSKGEIEVAVHDTGVGISQADLRRIFKPFERAGTRPVPGTGLGL
                     AISKVIVEAHKGRLSVESVPGEGSIFRAAFPRPKGQETTAIVTAPDLSGPTVIHLNGA
                     AKSTVLLVEDDPATVQLVKQVLESDGYSIEHAQSVGAALASIKAHPPLLVLLDVRLGT
                     ENGLDVVKAIRKDPATRALPVVAMSAHAMSKDVKAGLAAGCNDYLVKPIGAKALLERV
                     PSIIRGVAAPSPTNQSSERRAVRQR"
BASE COUNT          752 a         1076 c         1153 g          766 t
ORIGIN      
        1 aggcggcctc atgcattttg ggtaggcgga tgtcatcagt ctgggcgttg tgacccccac
       61 ccagtcgaac tagccttgcc taacgcggag tcaactaccg tgaacaaggg gtgggcatct
      121 tggaaaggtc gcaatcacga agccaaggat ctgctctctc ctatcctgtt gggcccccgc
      181 aggcgcgcca tcgctcttcg cagatctccc cccagatcaa gtccactgag agtgaggcgc
      241 gcgtgctggt ggccgagccc tgtgacatct accgtcgagc tatcgtctcg ataatcgagg
      301 gaggccacag cggcttgatg ctctcgggtg aggtagccga tcggtcgcat gtgccacgag
      361 cgatacagat caccaacccg gatgtgctgg tcctgtgtct tcatgagatg actcgattcg
      421 atctcatccc acaggcgatc aacgccgttc cggggatccg tgtggtcgcc atctcctatc
      481 gcgacgatgg cgagacggtc gccaaggcga tccgtgcggg tgcaatgggg ttcctctcta
      541 agcgagcttc tccggaggag atcctcttca cgctcaagga ggctgcgagg ggcaaggttg
      601 ttgccgatcc gtcgcttttg acctcggacc tactctgggc tgtcgccgga gtggacaacc
      661 ggcgtgctct catcgagtca ctaacggatg gggagcgtaa agtactcgac ctgctcgcgc
      721 agggcatgac tgcgcgagcg atgggcgtta ccctgtttct gagcaagcga accatcgaag
      781 ggcgcctagt aagcatctat cgcaaactgg gcgtcgcagg ccgcatcgaa gcagcgctcc
      841 tatgggcgca tacgaaggag gcctcttgaa ccaagatctt gcggcactca actcaagcaa
      901 cgaccacggg gtcggtctgc gggtggtggc gaccaaagac ggcaacggtg tcgatgaggt
      961 cactcccaag aaaccgtcac tcaaactggt ccgtccagac gacccgggcc cgtcttcgac
     1021 cgaggttgta cacggtcaga tactcatcat cgaagacaac ccactgatat cactgacact
     1081 gcatgacgca ctttcgtcca tcggcctcaa ggtggtttcg gtcccaaccg gtagcgcggg
     1141 actcgcggct gcacgctctg gcgcgatcgg cgcgatcgtc ttagatcttc ttcttccaga
     1201 tatggacggg atggaggtgt tacgaaagct caagcgagat cccctgacag aggatgtccc
     1261 agtagtcatg cttactgctg tcggcacgac tggggaccgc aagactgcga tggcgcaagg
     1321 gtgcgttgct ttccttctca agcccccaaa cctcgctgaa ctggtgaacc tgctggacga
     1381 ggtaagcgtt cgaacggaga gccgatgaag tggcacttct ggtcccctcg tagagctttc
     1441 caagctatgc ctctccggca gaagatcttc gtgccgttcg cgtgcctctg cttgatgttt
     1501 ggtgcggttg gcacgttcgt ctacgcgagg gccacagttg taagggagcg ttcggttcgg
     1561 gcaggtcagt tgtcgagtgc aagtgcctac atctccttgc tggtcaagga ctcagctctg
     1621 tcccttgcgg cggcgtcgag gcgagccgta ttcacggaag gtttcgcgca ggcgctggct
     1681 gatcacgacg aagttcggct ctccaaactt cttcttccgg tggctgtgaa cgcaagacat
     1741 tcgttctacc tcgctcttga cgctaatgga tcgggcgcta ttgagctggc ctggcaggga
     1801 acaccggaac cgacacctcg gcctgcaggt gagtggggct cggaaccgtt gtttcgagag
     1861 gctgttaccc ggtcgggtga gtatgaactc acaccttcct tgctggagcg cgaaggcgag
     1921 tggttcattg ttgtcgccgc tcccgtgacc gtcactgatg gaggggcgag gggtctactc
     1981 gtggtgggcg acgcactcac accgctgatt cgggttccga aagttcagaa cctcatcttg
     2041 ggtttcatgg acactagggg aaaccctgtg gtcggtagct ttgctgccag gagccccggc
     2101 ggccccgccg aggtgttgaa tgcggtctcc aaggtcggcg gcaaacgata cgaaactcta
     2161 ttctcgcccg tgcagctggc gggcaggtcg atcggtactc tcacgatcgc agttccggcg
     2221 ccatcgggcc tcggtatgct cggtggtcaa ggtttcatca tcggcttctt ggccttggcc
     2281 acacttctga tggcattcgg cgtaggtagt ttcatctcgc ggtccctcac gagacgacta
     2341 gcagagctcg ccgatgccgc ctacgagttc cagtcgggtc gtagggatgt gagggtaaac
     2401 gcagacgggt cggacgagct ccacgaggtg gccgccgcat tcaacctgat ggcgactgat
     2461 ctgcagcagt cccacgacag cctagaagcg acgatcgaag agcggacggc ggaactgaag
     2521 cactcgctga gccgcctcga ccgtgccaat gcaaggctga ccaaagcaaa cgaggcgaag
     2581 tctgtctttc ttggcaacgt ctcacacgag ttgcggaccc ctctgagcgg catcctgctg
     2641 gcgtcggaga tgcttcatga ccggagcttc aagatgttct ccgaagagaa ggtcagagag
     2701 ctttcgggca agatccacgg aagtgggaga catctcttag cccttatcga cgacctgctc
     2761 gatctctcgc gcatagaagc tgggcgcctc gacctgaacc tccagtcgat atcactgagt
     2821 cccctgctcg ctgaagttcg tagctcgctc ggaccgatag cgtccgacaa ggggatcgct
     2881 ctcgacatac cttcaggaga gggacacacc gtcttcgctg acccagtaag gatgaagcag
     2941 gtcctcatca acctgatcac caatgcgatc aagttcaccg atgtcggcgg ccgcgtttgg
     3001 gcggagatag ttccttcgaa gggtgagatc gaggttgcgg tccatgacac gggagtcggc
     3061 atcagtcagg cggatctgcg caggatcttc aagccctttg aacgggcggg aaccagaccg
     3121 gtgccgggca ccggtctagg gcttgcgata tcgaaggtga tagtcgaagc gcacaaaggg
     3181 aggctttccg tagagagcgt tccgggcgag ggcagcatct ttcgcgctgc cttcccacgt
     3241 cccaaagggc aagaaacaac agcgatagtc acagcaccag atctatcggg cccaacggtc
     3301 attcacctga atggggccgc gaagtcaact gtgctgctcg tggaggacga ccctgcaacc
     3361 gttcaactcg ttaagcaggt actcgaatcg gacgggtact cgatagaaca tgcccagtcg
     3421 gttggcgccg ccctggcttc gatcaaggca catcctccac tgctggtact gctcgatgtc
     3481 aggttgggta ccgagaacgg tctggatgtg gtgaaagcga taaggaagga ccctgcgact
     3541 cgggctctgc cggtggtggc gatgagcgcc catgcgatgt caaaggatgt gaaggctggc
     3601 ctcgccgcag gttgtaacga ctacctcgtc aagccgatcg gggccaaggc actcctagag
     3661 cgggtgccga gcatcatccg gggggtagca gccccctcgc cgacaaacca gagctcggaa
     3721 agacgagcgg tccgccagag atgattc
//
LOCUS       DATDOZ010000109         2770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2192563, whole genome shotgun sequence.
ACCESSION   DATDOZ010000109 DATDOZ010000000
VERSION     DATDOZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192563"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..793
                     /locus_tag="VI541_02070"
     CDS             167..793
                     /locus_tag="VI541_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69723.1"
                     /translation="MSRINLLPEEVLERRRNRQQSLMLAVYFGIFLLVLVGVWLVRNG
                     QLNAQEERRDRALAQVATLQTKIAALEEFARLDQTVKAKAAVLATAMSDDVAWSRLLI
                     EVSMIIPGDSWLTSFNGVAVPASAAAAATPVGPKSLGNLTFSVVTFDFRGVAEWITRF
                     EGLRSLQNIWVPSATKGTIGTRDVVNYSSTADLSVGAATSRYQSGPPS"
     gene            790..1383
                     /gene="pilO"
                     /locus_tag="VI541_02075"
     CDS             790..1383
                     /gene="pilO"
                     /locus_tag="VI541_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HLF69724.1"
                     /translation="MKRPYLIFGAGIVLVGILYFFVVFQPQGNHISQTTDEAVAAEIR
                     RTQMEADLKHLQELQQNAPKLREQANILDEAMPNDPQLAKFIRQVQDAADGSGIEFLS
                     IAPTPPTQSPDQPEVKLVAITMSISGGYFAVQDFIVRLETLSRAVKVGSINLSLGDAV
                     SSSSSPPLKVSLTMQMFVANPNPVPATVPGVTTTAGA"
     gene            1387..1974
                     /locus_tag="VI541_02080"
     CDS             1387..1974
                     /locus_tag="VI541_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69725.1"
                     /translation="MSTETPTIEEPKKQIHPAIPAALVVLIVGLFVFFVIKPLFFPSR
                     SNVVPPPVTSGGPSASPQPSPSPSPAAAPAETFEVFESKDPFRPLVVEFAAGAPGGTT
                     SGTSTGSTGSTSTGGGAGGASAPTGGQSVTLVDVFTQDGTEKAQVKVGSTVFTVAAGE
                     VFADNFKLLSISGTCATMLHGDDKFTLCEGEEVFK"
     gene            1994..>2770
                     /gene="aroC"
                     /locus_tag="VI541_02085"
     CDS             1994..>2770
                     /gene="aroC"
                     /locus_tag="VI541_02085"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003977330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLF69726.1"
                     /translation="MSLLRFLTAGESHGPALTVIVEGMPAGVPLRASEITDELVRRRL
                     GYGRGPRMKIERDEIELLSGVRAGLTLGSPISVVIRNTEWNKWERVMAAEGESAGNRL
                     TEPRPGHADLAGLLKYGATDARDILERASARETAARSAAGAMAKALLARLGIAVISHV
                     TAIGKASATADMRPAPEDLDQIDSSPVRCLDPVVEEQMVREIESASEAGDSLGGVFEV
                     LAFGVPVGLGSHVHWDRRLDSRLAGAVMSIQAIKGVEIGDG"
BASE COUNT          554 a          814 c          845 g          557 t
ORIGIN      
        1 ctccgcctcc cgatcgaaga ctcgaaggcg atccatcgac tgcagatcac ccggctgaga
       61 ctcacggagg agcagatatc tgaagccgag cctctgatgg gcaccgctat cggtacggct
      121 ctgggggggt tcgaatgaga cctcgaaacc agggaacggc gaagctatga gtcggatcaa
      181 ccttcttccg gaagaggtac tcgagcgccg gcgtaaccgg cagcagagcc tcatgctcgc
      241 tgtctatttc ggcatcttct tactcgtcct tgtgggcgtc tggttggtgc ggaacggcca
      301 gctgaacgct caagaggaga ggagagacag ggcgctcgca caggtggcta ctcttcagac
      361 aaagatcgca gctctcgagg agttcgctcg tttggatcag acggtcaagg caaaggctgc
      421 ggtcctcgcc acagcgatga gcgatgatgt tgcgtggtcc cgcctgttga tcgaggtttc
      481 catgatcatc ccgggagact cttggttgac gtccttcaat ggggtagctg tacctgcttc
      541 ggccgctgcg gctgcaactc cggtagggcc caagtccctg gggaacttga ctttctctgt
      601 cgtcaccttc gactttaggg gtgtcgctga gtggatcacc cgattcgagg gactaaggag
      661 ccttcagaac atctgggtac cctcggcaac taaggggacg atcggaacgc gtgatgtcgt
      721 gaactattcg agtacggccg atctctccgt cggagccgcg acctctcggt accagtcggg
      781 acccccatca tgaaacgccc atacctgata ttcggtgcag ggatcgtgct ggtggggatc
      841 ctctacttct tcgtggtctt tcagccgcag ggcaaccaca taagtcagac aactgacgaa
      901 gcggtggccg ccgagatccg aagaacacag atggaggcag atctgaaaca cctccaagag
      961 ctacagcaga acgcccccaa gctccgcgag caggccaaca tactcgacga ggccatgccc
     1021 aatgatccgc aactcgcaaa gttcatccgt caggttcagg atgcagcaga tggctccggc
     1081 atcgagtttc tctccatcgc tcccacgccc cccactcagt cgccagatca gcccgaagtt
     1141 aagttggtgg cgataacgat gtcgatatcg ggtggatact tcgcagtcca ggatttcatc
     1201 gtccgactcg agacactctc acgggcagtg aaggtgggct cgatcaacct ttctttaggg
     1261 gatgcagtga gttccagctc ctcgccgccc ctaaaagtga gccttacgat gcagatgttc
     1321 gtggcgaatc cgaacccggt tcccgcgact gttcctggtg ttacaactac ggcgggggcg
     1381 taagagatga gtactgaaac gccaacgatc gaggagccga agaagcagat ccatccggcc
     1441 atccctgcag cgctggtggt cctgatagtt ggtctcttcg tcttcttcgt tatcaagccc
     1501 ctcttcttcc cctcgcgctc gaacgtcgtc ccaccacccg taacctcggg aggtccctcg
     1561 gctagcccgc agccgtctcc gtctccctca cccgccgcgg ctccggcaga aaccttcgaa
     1621 gtattcgaat ccaaagatcc cttcaggcca ctcgtcgtcg agtttgccgc cggggctccc
     1681 gggggcacga cctcggggac gtcaaccggg tccacgggtt ccacttccac cgggggggga
     1741 gcaggtggtg cttcggctcc aaccggtggc cagagcgtca cacttgttga tgtgttcacc
     1801 caagatggca ccgagaaggc tcaggtcaaa gtggggagca ctgtcttcac ggtcgcagct
     1861 ggcgaggttt tcgcagacaa cttcaaactt ctgtcgataa gtggtacctg cgcgaccatg
     1921 ctgcacggag acgacaagtt caccctctgt gagggcgaag aagtcttcaa gtagccccaa
     1981 gctcaacaac atcttgtccc tgctccgatt cctgaccgca ggcgagtcgc atggtcccgc
     2041 attgaccgtc atcgtcgagg gtatgccggc gggtgtgcct ctacgcgcta gcgagatcac
     2101 cgatgagttg gtgagacgca ggctcgggta tgggcggggc ccccgcatga agatcgagcg
     2161 cgacgagatc gaactgctca gcggggtgcg agcgggtttg accctgggga gccccatcag
     2221 cgtcgtcatc cgcaataccg agtggaacaa atgggaacgg gtcatggccg ctgaagggga
     2281 gtcggcaggc aaccgtttga ccgagcctcg cccagggcat gcggacttgg ccggactcct
     2341 caagtacgga gctaccgacg cacgcgacat cctcgaacga gcaagcgcgc gagagacggc
     2401 tgctcgttcg gcggcagggg cgatggctaa ggcgcttcta gcgcgattgg ggatcgcggt
     2461 catctcgcac gtcaccgcga tcggcaaggc cagtgctacg gcagatatgc gcccagcacc
     2521 cgaggacctg gatcagatcg actcatctcc agttcgttgt ctggatccgg tcgtcgagga
     2581 gcagatggtt cgtgagatcg aatcggcatc tgaagcgggc gactcactgg gtggtgtctt
     2641 cgaggtcctc gccttcgggg tgcccgtcgg tcttggctcg catgttcatt gggatcgaag
     2701 gttggactcc aggttggctg gggcggtcat gtctattcag gcgatcaagg gagtcgagat
     2761 cggcgatggc
//
LOCUS       DATDOZ010000110         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_349892, whole genome shotgun sequence.
ACCESSION   DATDOZ010000110 DATDOZ010000000
VERSION     DATDOZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349892"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /gene="ftsH"
                     /locus_tag="VI541_02090"
     CDS             <1..1904
                     /gene="ftsH"
                     /locus_tag="VI541_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLF69727.1"
                     /translation="LVVDARLLEHDSTIVMRIRPEPPAPPPVPAPDGSIAPGTTPPEG
                     AVTPTVVAKPKPPYEAWLAQPRTGSLTFEIVDSLRKGGAKITVDPQNTKSVLTFLAQF
                     LWPLLILATVFGFIFLVITGGGGGAADFLGFSKFAAKIQRRKKGTKGVITFKDVAAID
                     EGLTELQEVVEYLMNPTQFAEFGARAPKGVLLAGPPGTGKTLLAKAVAGEASVPFIQL
                     SGSEFVESLVGVGAARVRSLFQQARDLAPCIVFIDELDAAGRQRGAGVGGGNDEREQT
                     LNQLLVEMDGFSASVGIAVLGATNRPDILDPALLRPGRFDRQIVIDVPDVRGREEILN
                     VHVRGRPLAADTDLSSIAKQTPGFTGAELANIINEAALLAVRRKRHEITQEDLEEAVD
                     RVLAGPERKSHILTDEEKIMIAYHEAGHAVVARGAGQETGVLKLSIVARGLQLGHAST
                     FSSADRMILLRTEMEGQLTTLLGGLAAEKLVFGDITTGNTGDLEKATNKARKMVGTLG
                     MSAEIGPVQIIHEGQVFMGRDFSAMQNTSGEILNVMDREIRRVLDEAEGRATEICRTN
                     RAILDEIVGLLLERETMLGPELEPILSKVKTLALPAPLAGLLGSTNGGPRKRRHMARR
                     PVDIAALDE"
     gene            1907..2524
                     /locus_tag="VI541_02095"
     CDS             1907..2524
                     /locus_tag="VI541_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69728.1"
                     /translation="MQKRLRRRYLLAGAFLLLVLAIPTVRQIINRVGNTAADRKTTSA
                     DGASGSGAQGRTSPTPTSTAAGAGKAAKTYSPRPTLTPSIAIPYDSAFGTFTSDGGAD
                     YPHPAGCDLRRGVLCSVSMHGRYILTNYEGAIIKVGAFENDTDVPVVSAQISILKGSS
                     VFFQNLAYTPSSTAVTVRFRTILLTTDGRFIFERSPLPPSIEVAP"
BASE COUNT          511 a          846 c          766 g          462 t
ORIGIN      
        1 ggctggtcgt cgacgctcgc ctcttagagc atgactcgac gatcgtcatg cggatacgcc
       61 cggaaccccc cgcgcctcca cctgttccgg ctcccgacgg ctccatcgcc cctggcacga
      121 cgccgcccga gggcgccgtc acccctactg ttgtggccaa acccaagccg ccctacgagg
      181 catggctcgc acagccgcgc acgggctcgc tcactttcga gatcgtcgat tcgctgcgca
      241 agggcggtgc gaagatcacc gtcgatccgc agaacaccaa gagcgtcctg accttcctag
      301 cgcagttcct ctggccactt ctcatcctcg cgacagtttt cgggttcatc ttcctcgtca
      361 tcacaggcgg cgggggcgga gcggcggact tcctcggatt ctccaagttc gccgcgaaga
      421 tccagcgccg aaagaaggga accaagggag tcatcacgtt caaggacgtt gctgcgatcg
      481 atgaaggcct gaccgaactt caagaggtcg tcgagtatct gatgaacccg acgcagttcg
      541 ccgagttcgg agcaagggcc ccgaaaggcg tattgctggc cgggccccct ggtacgggta
      601 agacactcct cgccaaggcc gttgccggag aggcatctgt gcccttcatc cagctctcgg
      661 gttccgagtt cgtcgagtct ctcgtcggtg tcggtgcggc ccgtgtccga agcctcttcc
      721 agcaggctcg agacctcgca ccatgcatag tcttcatcga cgagctggac gctgccggtc
      781 gtcaacgtgg tgccggcgtc ggtggtggta acgacgaacg tgagcagacg ctgaaccagc
      841 tcctggtcga gatggacgga ttctctgctt ccgtcggtat cgccgtgctt ggcgcaacca
      901 accgccccga catcctcgac cctgcacttc tgcgtcccgg acgcttcgac agacagatcg
      961 tcatcgacgt tcccgatgtc cgtggtcgcg aagagatcct gaatgttcat gtccgtggcc
     1021 ggcctcttgc ggcggacacg gacctctcgt cgatagccaa gcagacccct gggttcacgg
     1081 gggccgagtt ggcgaacatc atcaacgaag ctgcgctgct ggcggtgcgt cgcaaacgcc
     1141 acgagatcac acaagaggat ctcgaggaag cggtcgaccg tgtgctcgct ggtccggagc
     1201 gcaagagcca catcctcaca gacgaagaga agatcatgat cgcctaccac gaggctggac
     1261 atgcagttgt cgcgcgaggt gcgggccagg agaccggtgt actcaagctc tcgatcgtcg
     1321 cgcgtggact tcagctcgga cacgcgtcga ccttctcgtc tgcagaccgc atgatccttc
     1381 tgcgcaccga gatggagggg cagctcacaa cgctgctcgg gggcctggcc gccgagaagt
     1441 tggtcttcgg agacatcacc accggcaaca ccggcgacct cgagaaggcc acgaacaagg
     1501 cccgcaagat ggtcggtacg ttaggcatga gtgccgagat cggacccgtt cagatcatcc
     1561 acgagggaca ggtcttcatg ggccgagact tctcggcgat gcagaacacg tccggcgaga
     1621 tcttgaacgt catggaccgc gagatccgcc gagtactgga tgaggccgag ggaagagcga
     1681 ccgagatctg ccggaccaac agagcgatac tcgacgagat cgtcggcttg ctgttggagc
     1741 gtgagaccat gctcggcccc gaactagagc cgatcttgag caaggtcaaa acacttgccc
     1801 tgccagcacc actggcaggc ttgctcggct caaccaacgg ggggccccgc aaacgccgcc
     1861 acatggccag acgtccggtg gacatcgccg ccctggacga gtaaccgtgc aaaagcgcct
     1921 tcgcagacgc tatctgttgg cgggggcctt cttgttgctg gtcttggcga tcccaactgt
     1981 tcgacagatc atcaaccggg tggggaacac cgcggccgac cgaaagacta cctcggcgga
     2041 tggagcgtcg gggtcggggg cccagggccg gacctcaccc acccccacat ccaccgccgc
     2101 cggggcgggc aaggccgcca agacctacag cccacgcccg acccttaccc ccagcatcgc
     2161 catcccttat gacagtgctt tcggaacatt cacctcagac ggaggggcgg actaccccca
     2221 cccggccgga tgcgatcttc gacgcggagt tctctgcagc gtatcgatgc atggtcgcta
     2281 catcctgaca aactacgaag gggccatcat caaggtcgga gccttcgaga acgacaccga
     2341 tgtgcccgtg gtcagcgcgc agatctctat attgaaagga tcctcggtat tcttccagaa
     2401 tctggcatat accccctcgt ctacggccgt gacggttagg ttccgcacga tccttctgac
     2461 caccgatggc cgcttcatct ttgagcgcag cccactccct ccctccatcg aggtcgctcc
     2521 ctagcgagaa gttcgcacaa aaaactgctc ccgggcagga atcgggcaag cggtgtcgaa
     2581 actct
//
LOCUS       DATDOZ010000111         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1142079, whole genome shotgun sequence.
ACCESSION   DATDOZ010000111 DATDOZ010000000
VERSION     DATDOZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142079"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..841)
                     /locus_tag="VI541_02100"
     CDS             complement(68..841)
                     /locus_tag="VI541_02100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF69729.1"
                     /translation="MKLFRREGRSAEIPLPPADLMFEIGASRVEEYTDIGNEFMGYFV
                     ELASLTPDSDVLDVGCGVGRMALPLTRFLSRRGSYRGFDTWGRSIDWCKENIQSRFRN
                     FRFDLVDIYSKRYNPGGSVKASEFVFPYADDSFDFAALTSVFTHMLPSDLQRYLGELA
                     RVLRPGGRCLITYFLLNQESLAAHDRGETILRFPVEKQGYRLANEEIPEEAIAYDETF
                     VTAAHISASLPIQEPIYFGEWTGRKEFLSGQDIVIAKRT"
     gene            1000..2202
                     /locus_tag="VI541_02105"
     CDS             1000..2202
                     /locus_tag="VI541_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLF69730.1"
                     /translation="MSESTFPLDIVVIGGCGHVGLPLGIAFAHRGLKVALYDTNSAAV
                     ATVNSGELPFDEPGAADILREVVGSGLLVAHADPSPISRAENVVVVIGTPVDEHLNPD
                     PQAVPNAIMQLADHFRDGQLLVLRSTLYPGVTALVERSIAETGLSIDVSFCPERIAEG
                     RAMTELFDLPQIVGGRAETALDRAEKLFRNLTQQTVRLLPEEAELAKLFTNTWRYIKF
                     ATANQLYMIANDFGLDFEKIRTALAYDYPRAADMPRAGFSAGPCLFKDTMQLAAFNNN
                     NFMLGHSAMMVNEGLPLYVVARLQDKMNLRNKTVGILGMSFKAESDDIRSSLSYKLKR
                     ILRFKAGAVLTTDPFVKNDPELLPLQEVLDRSDVVMIGTPHKAYADLEIAVPVIDIWG
                     SMGRGVRV"
     gene            2199..>2543
                     /locus_tag="VI541_02110"
     CDS             2199..>2543
                     /locus_tag="VI541_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF69731.1"
                     /translation="MRPRVSVVIPVYNEGEAITPSLDRIFEAVSLPCEVLVVFDSLED
                     TTVPYLEKYAADEARLIPTLNGYGRGAAKAIRFGMDRAQADVIVVTMADGSDDPQQID
                     LLTRLVERGVVVA"
BASE COUNT          561 a          742 c          706 g          534 t
ORIGIN      
        1 ggtgggatcg ttcaggtggt ctgctccccg atcttctatc gagaccgacg agaaatcgcg
       61 gccgaggtta agtccgcttc gctatgacga tgtcttgccc gctgagaaac tcctttcgac
      121 cagtccactc gccaaagtag atcggctcct ggataggaag agaggccgat atgtgcgccg
      181 cagtcacgaa ggtctcgtca taggcgatcg cctcttccgg tatctcttca ttggccagtc
      241 ggtaaccttg cttctcaaca gggaatctca ggatggtctc tcctctatca tgggcggcga
      301 gggactcttg gttcaacagg aaatatgtga tcaggcaacg accccccggt cgaagaacgc
      361 gagcgagctc acccaggtat ctctgcagat cagatggaag catgtgagtg aagactgaag
      421 tcagggcagc gaagtcgaat gagtcatccg cgtaaggaaa gacgaactcg gatgccttca
      481 ccgagccccc agggttgtat ctcttgctgt agatatcgac gagatcgaac ctgaagttcc
      541 tgaaacggct ttggatgttc tctttacacc aatcgatgct tcgaccccag gtgtcgaatc
      601 ctcgataact acccctgcgg ctcaggaatc gggtgagggg taacgccatt cgccctaccc
      661 cacaaccgac atcgagaaca tcggagtctg gggtcagtga tgcgagttcg acgaaatacc
      721 ccatgaactc attgccgata tcggtgtact cctcgacgcg actcgccccg atctcgaaca
      781 tcaggtcagc cggtggcaag gggatctctg cactccggcc ttccctgcga aacagcttca
      841 tcgatgaaac tcccttctcg ggacgggcgt cacaatcaca tcgagcgatc ctcttcacaa
      901 gatatctgcc tacggcacca ccctgaagga cccgtgtata ttggcaccta ccggtcgaac
      961 tgaagaagca acggttacga cgtcgtcttg ggggcacaca tgagcgaatc tacctttccg
     1021 ttagacatag ttgtcatagg tggctgcggc catgtcgggc tccccttggg gatcgccttt
     1081 gcccaccgag gcctgaaggt tgccctctac gacacgaact ccgcggcagt cgcaacggtc
     1141 aacagcggag agcttccatt cgacgagcct ggtgccgctg acatcctgcg cgaagtcgtt
     1201 ggatctggcc ttctcgtggc gcacgctgat ccatcgccga taagtcgagc tgagaacgtt
     1261 gtcgttgtga tcggtacccc cgtggacgaa cacctgaacc cagatcctca ggcggtccct
     1321 aacgcgatca tgcaactcgc ggaccacttc cgcgacggac aactgctcgt cctaaggagc
     1381 acgctgtatc cgggggtgac tgctctggtt gagcgatcca tcgccgaaac tggcctgtcg
     1441 atcgatgttt ccttctgccc agagaggatc gcggaaggcc gagcgatgac tgaactgttc
     1501 gatcttcccc agatcgtcgg cggtcgcgca gagaccgcgc tggacagggc tgagaaactg
     1561 ttccgcaacc tcacccagca gactgtgcgg ctcctcccag aagaggccga actagccaag
     1621 ctcttcacaa acacctggcg gtacatcaaa ttcgcgaccg ccaatcagct gtacatgatc
     1681 gcgaatgact tcggcctcga cttcgagaag atcagaaccg ctctggcgta cgactaccct
     1741 cgggcggccg acatgccgag ggctggattc tcggctggcc cttgcctctt caaagacacg
     1801 atgcaactcg ccgcattcaa caacaacaac ttcatgctcg gtcactcggc catgatggtg
     1861 aacgaaggtc ttcctctcta cgttgtcgca cgactgcagg acaagatgaa cctacggaac
     1921 aagactgtgg gcatcttggg gatgtcattc aaggccgagt ccgatgacat ccgatcgagt
     1981 ctcagctaca agctcaagag gatcctgcgg ttcaaggctg gagccgttct aacgactgac
     2041 ccattcgtga agaatgaccc tgaattgctt ccgctacaag aggttctgga caggtcagat
     2101 gtagtgatga tcggcacccc tcacaaggca tacgcggatc tcgaaatcgc ggtgccggtt
     2161 atcgatatct ggggctcgat gggacgtggc gtaagagtat gaggccacgc gtttccgtcg
     2221 tcatcccggt ttacaacgag ggggaagcga taacccccag cctcgatcgg atcttcgagg
     2281 ccgtctctct gccatgtgag gttctggtgg tcttcgactc cctcgaagat acgacggtcc
     2341 cctacctgga gaagtatgca gccgatgagg cccggctcat cccgactctc aatggatacg
     2401 gtcgtggtgc tgctaaggcg atccgattcg ggatggacag agctcaagcc gatgtcatcg
     2461 tcgtgacgat ggccgatgga agtgacgacc cccaacagat cgacctcttg acgaggctcg
     2521 tcgagagagg ggtcgttgtg gcc
//
LOCUS       DATDOZ010000112         8529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1917355, whole genome shotgun sequence.
ACCESSION   DATDOZ010000112 DATDOZ010000000
VERSION     DATDOZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917355"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..752
                     /locus_tag="VI541_02115"
     CDS             351..752
                     /locus_tag="VI541_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69732.1"
                     /translation="MIGRVRAVTLILGILLAIVGTLPNFTPFHRSGGPVTANLPQREP
                     QGVTRPPLVGRHSGQPSADRSIAQATPSDAPGLATAHDPAPDPAPDPAPEQSDPAPRS
                     DVGLISTSVSLVTSLLSDALGLLAIGSSSSR"
     gene            802..1749
                     /locus_tag="VI541_02120"
     CDS             802..1749
                     /locus_tag="VI541_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF69733.1"
                     /translation="MGLLDGKVAIVTGAGRGIGRGEALLLAREGARVVVNDVGASLSG
                     EGHDRSPADEVVGEIKALGGDAYADDTDVTNFDGVSAMISRAVEQFGRLDILVNNAGI
                     LRDKTLIKMAEAEWDAVIGVHLKGTFNCSHHAAAYWRGVYDETQTPVGGRIINTSSPS
                     GLYGNFGQSNYGAAKAGIAAFTVIVAQELLKIGVTVNAVSPSARTRMTEQAFGTIAAP
                     EGGFDLFAPENIAPIVAYLATDDAAGITGQVFGVQGGSIALLRGWMPEAVMDKGDAWE
                     PSELVEAIEKIVPGKRAEPDPMAARLAAGQQSLISGAKK"
     gene            1760..2935
                     /locus_tag="VI541_02125"
     CDS             1760..2935
                     /locus_tag="VI541_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69734.1"
                     /translation="MDLTWSAKDEEFRAEVRDYLEMNVPRDIHAGEETFEARREWQRK
                     MAQDGWVAIHWPTEYGGRGASLLQTVIFNEEYTRVRGPSLPNELGIGLLGPTLMVHGN
                     DEQKRRYLQKILTAEEIWCQGFSEPDAGSDLAALQCRADLDGDEFVVNGQKIWTSYGR
                     YGDFIFTLVRTDNTGVKHAGISFLLIDMHSPGVEVRPLKEMTGESLFAEVFFTDVRVP
                     RSNLVGELGEGWRIAMTTLTFERGAGGLAQSVRLRNSVKDLITDAKKIKRNGGSAFDD
                     PLVRSKIGQRLVEIEVYRYNTLRAISAIANGQPAGPEASINKLYWSTWHKAFMEDAIE
                     ILGPHARAAGLSDDIDGQRWMKEFLFSRAGTIYAGTSEIQRNILSERVLGLPKEGKS"
     gene            2935..3930
                     /locus_tag="VI541_02130"
     CDS             2935..3930
                     /locus_tag="VI541_02130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69735.1"
                     /translation="MDFSLNEDQLSLQKSAREFLTKECAIEMVHRSFDGPDGDAPELY
                     AHMADLGWLGLAVPEEHGGVGFGVVEQAVLFEQLGYFNAPGAFFSNTALVIPALIAVG
                     ATDQIAEVVEGKTRYAYVQDPDFVLDGQLADKFVVVSDEVRIIDASDATVTALESIDG
                     TRRFASVQTRAGAGHIIGSADGLERVLDIASTLLCAESVGGMQRVLEQTVEYAKVRTQ
                     FDRPIGSFQAVKHQLADALLRVESSRSAALYAAWANSVGAPDAKFASSVAKAYIAEAA
                     VWVTGAGVQLHGGIGFTWEHDSHLYFKRAVTNAALLGDAAHHRARALSLSPFLGE"
     gene            3996..4940
                     /locus_tag="VI541_02135"
     CDS             3996..4940
                     /locus_tag="VI541_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HLF69736.1"
                     /translation="MDLFTLAAQQYGVITRRQALERGMSPSALDRSLDRGEWIPLAPG
                     VYRHACSPPSWRQELAAAHLAMGWPSAVSHRSAAALWKLSGFGEDMVELTCILGSAHT
                     PEPRWMVHRTNWVESQITQIDGIPVTNVERTLMDLCAVIPLGRVARGLDEALMRGLTS
                     VESIRGWLGEVGGRGRRGAGILRNLVDERAPMPRGLESVLEVKVMRIIRSAGLPLPTP
                     QYELRDGSRVLARLDFAYPSRKFAIEADGFKYHGGRTAWRKDLDRNNVIAAMGWQVLH
                     VTWDDLRDRRAKFISDVTAFLGEKPPVGGGFSPKFERG"
     gene            complement(4937..5353)
                     /locus_tag="VI541_02140"
     CDS             complement(4937..5353)
                     /locus_tag="VI541_02140"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HLF69737.1"
                     /translation="MDDESVETKADGGVFDPPKLLRIASLAREVLEELRKMDPATTPA
                     EQFAGLYLKVKKQLESALPKELIDELESIQLDLPFADGATQQEVLVAYSGLIGWLGGL
                     FQGLQASVQSHVLELAEKSTAEAAEAAEAKPKEGYL"
     gene            5417..6625
                     /locus_tag="VI541_02145"
     CDS             5417..6625
                     /locus_tag="VI541_02145"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HLF69738.1"
                     /translation="MPDAVIVDGTRSPIGRAYKGALKDVRPDDLGGYIVQKLMEKFPD
                     LDPTTIDDVMVGCGLPHGESGFNVARPIVLLSGLPNSVPATTVNRFCSSSLQTIRMAF
                     HAIKAGEGSTFISAGVESSSRVELLPRGDVTVLNPRLSPLFGGEYIADIYISMGETAE
                     NVADRFDVSREDMDRFAQQSQERAVSAQKDGFFTKEITPIPLATSGERTIIQEGGDSL
                     LVEHDDGPRPSSTYEKLASLEGAFRPGGKVTAGNSCPLNDGAAAVLVMEEAKARSLGL
                     KPRVRIIASAVSALEPEIMGVGPIEAVRKALAIAGMTIEDIDVVELNEAFAAQVLPVC
                     RELGIDPFSDKLNPHGGAIALGHPFGMTGSRIMNTLMNDLETLDRTIGLETMCVGGGQ
                     GMAMIVERIN"
     gene            6625..7083
                     /locus_tag="VI541_02150"
     CDS             6625..7083
                     /locus_tag="VI541_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLF69739.1"
                     /translation="MTTIIKGKQELLGRVGDELGVSEWHEITQDQVNKFADATGDHQW
                     IHVDPEKAALGPFGTTIAHGYLTLALGPKLSWETVTVEGVQMGINYGSDRVRFITPVK
                     VGARIRMRIKLADLKEVEPNGLQATFENTFEIEGEDKPACVAQVISRYYF"
     gene            7132..>8529
                     /locus_tag="VI541_02155"
     CDS             7132..>8529
                     /locus_tag="VI541_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69740.1"
                     /translation="MINPGRDDTKDVQLSKGLVRRVFKFAKPYRGSIVLAMIVILAAA
                     VATGLGPQIIRRIIDEAITHRDKAKLNLLIALFLGLALAGSFLGIAMRYLLSRIGEGL
                     IFDLRSSLFDHVQRMPVAFFTRTQTGALISRLNNDVIGAQRAVTETTAGLLQVIADLV
                     VTLALMWSMQPRLTVISLLVVPLFVLPIRSMGKVLQRYVRQQMEFNASMNTQMTERFQ
                     IGGALLVKLFGNYVKERDHFAQKAGNVRDLGVKTAVIGRLFFVTFSVVSAFGTAMTYW
                     LGGRMAISGEIKVGVIVAFSLYLGRLYGPITGLSNTPVEVKTALVSFDRVFEVLDFPS
                     AIQQKPDAVELTDPRGKVEFVGVRFRYPPGSETSIPSLEEGRIDTKYDPEAWVLKDVS
                     FTIEPGQMFALVGPSGAGKTTISMLVPRLYDAVDGTVLIDGIDVKDLTLPSLAASVGV
                     VTQDSHMFHDTIRANL"
BASE COUNT         1630 a         2566 c         2642 g         1691 t
ORIGIN      
        1 cctaacgagc tcgatcggta ccatcgcccg ggcgcacgga gaggtaaccg tcgacagcat
       61 cacggtctcc taggtcaaac cattacgcga gaggggcccc catttggggg cccccctcca
      121 agtttcgata cctccggttt ctggggcctc gtctgtccaa ggcttcgcag cctctgatcc
      181 gcggcaagcg cgatgcgttc cgctcgatac cgagtaacca gctagtcatt agtgactatc
      241 aaagatagcc acgatgacca ttcatcctgc agggcccctt tgtgtaggtt cgtatgtatt
      301 ggaaacgctt tgtgactcag cacccagggg agcaggtggg cgagaagatc atgattgggc
      361 gagtcagggc cgtaacgctc atcctcggta tcttgcttgc gatagtgggg acgctcccca
      421 acttcacccc cttccaccgc tcagggggcc ctgtaaccgc caaccttcct cagcgagagc
      481 cccaaggggt cacccggccc ccactggtgg gccgtcattc gggccagccc tcagccgacc
      541 ggtcgatagc gcaggccact ccttctgacg ctcccgggct cgcaacggca cacgatccag
      601 cccccgatcc agcccccgat ccagcccccg agcagagcga tcccgcgccc cgcagcgatg
      661 tcgggctcat ctcgacaagc gtgagtctgg tgacatcact ccttagcgat gcgctcggtc
      721 tgttggcgat cggttccagc tccagtcgat aagttcgtcg cgagtcgcgc aacgagtgca
      781 ttccgatagc atctgactcc aatgggacta ctagatggca aggtagcgat cgttaccggg
      841 gccggtcggg gcatcggtcg gggtgaggct ctgttgttgg cgcgagaggg ggcccgcgtc
      901 gttgtgaacg atgtgggggc ctcgctgtct ggtgagggtc acgaccgcag tcccgctgac
      961 gaggtcgttg gcgagatcaa ggccctcggc ggcgatgcgt atgccgacga taccgatgtc
     1021 accaacttcg atggcgtgtc cgcgatgatc tcgcgggcgg tcgagcagtt cgggcgcctc
     1081 gacatcctgg tcaacaacgc aggcatcctt cgcgacaaga ccctgataaa gatggccgag
     1141 gccgagtggg atgccgtcat cggcgtgcat ctcaagggga cgttcaactg ctcgcatcac
     1201 gctgccgctt actggcgtgg cgtctacgac gagactcaga cccccgttgg gggccgcatc
     1261 atcaacacgt cctcgccgtc gggtctgtat ggcaacttcg ggcagtccaa ctatggcgcg
     1321 gccaaggctg gtatcgctgc gttcaccgtc atcgtcgcgc aggaactgct caagatcggc
     1381 gtgaccgtga atgcagtttc gccgtcggcg cgcactcgca tgacggagca ggcattcggc
     1441 acgatcgcgg cccccgaggg tggcttcgac ctcttcgccc ccgagaacat cgctccgatc
     1501 gtcgcctacc tcgcgaccga cgacgcggcg ggcatcaccg gtcaagtctt cggcgtgcag
     1561 ggcgggagca tcgcccttct tagaggctgg atgcccgagg ccgttatgga caagggcgat
     1621 gcctgggaac cctccgagtt ggtcgaggcc atcgagaaga tcgtgccggg caagcgggct
     1681 gagcccgacc ccatggcggc gcgcttggcc gcagggcagc aatctttgat ctccggagcc
     1741 aagaagtgat gggagcgata tggatctgac ttggagcgcg aaagacgaag agttccgcgc
     1801 ggaggtccgc gactacctcg aaatgaatgt cccccgcgac atccacgcgg gcgaggagac
     1861 attcgaggcg cggcgagagt ggcagcgcaa gatggcgcaa gatggatggg tcgcgatcca
     1921 ctggccgacc gagtacgggg gccgcggcgc gtcactactt cagaccgtga tcttcaatga
     1981 ggagtacacg cgagtccggg ggccgtcgct gccgaatgaa ctcggcatcg gtctgctcgg
     2041 tcccactctg atggtccatg gcaacgacga gcagaagcgt cgctatctcc agaagatcct
     2101 gaccgccgag gagatctggt gtcaggggtt ctccgaacct gatgccggaa gcgaccttgc
     2161 tgcgctccag tgccgagcgg atctcgatgg cgatgagttc gtcgtgaatg gtcagaagat
     2221 ctggacctcg tatggacgct acggtgactt catcttcact ctggtcagga ccgacaacac
     2281 gggcgtcaaa catgctggca tctcgttcct tctgatcgac atgcactcgc cgggtgtgga
     2341 ggtgcgaccc ctcaaagaga tgaccggcga gtctctcttc gctgaggtct tcttcaccga
     2401 tgtccgtgtg ccgcgcagca acctcgtcgg cgagttgggt gaggggtggc ggatcgccat
     2461 gacgacactc acattcgagc gcggggccgg gggccttgcc cagtcggtca ggctccgaaa
     2521 ctccgtcaaa gacctgatca ccgacgcgaa gaagatcaag cgcaacgggg gctcagcgtt
     2581 cgacgacccg ctcgtccgat cgaagatcgg gcagcgactc gtcgagatcg aggtctatcg
     2641 gtacaacaca ttgcgggcta tctcggcgat cgcgaatggc cagcctgcgg gccccgaggc
     2701 ttcgatcaac aaactgtact ggtcaacttg gcacaaggcc ttcatggagg atgccatcga
     2761 gatactcggc ccccatgcgc gtgccgcagg tctgagcgac gacatcgacg gtcaacgatg
     2821 gatgaaggag tttctcttct ctcgtgcagg aaccatctat gcggggacat ccgagatcca
     2881 acgcaacatc ctctcggaac gtgtcctcgg tctgccgaag gaggggaagt cctgatggat
     2941 ttcagcctta acgaagacca actttcactg cagaagagcg cccgtgagtt tctgacgaag
     3001 gagtgcgcga tcgagatggt tcatcggtcc ttcgatgggc ccgatggcga tgcacccgaa
     3061 ctctacgcgc atatggctga cctcgggtgg ttggggctgg ctgtccccga ggagcatggg
     3121 ggcgtcggtt tcggagtggt cgagcaggcg gtgctcttcg aacagttggg ttacttcaac
     3181 gctcccggcg ccttcttctc gaacaccgct ctcgtgatcc ctgccctgat cgcggtcggg
     3241 gccacggacc agatcgccga ggtagttgag ggcaagacgc gctatgccta tgtgcaggac
     3301 cccgacttcg tcctcgacgg gcagctcgcc gacaagttcg tcgtagtctc cgatgaggtc
     3361 cgcatcatcg acgcgagtga tgcaacagtc acggccctcg agagtatcga tggcacacgg
     3421 cgattcgcat cggttcagac gcgagcgggg gccgggcata tcatcggatc ggccgacggt
     3481 ctcgaacgcg ttctcgatat cgcatcgacg cttctgtgcg ccgagtcggt cggcggcatg
     3541 cagagagtct tggagcagac cgttgagtat gcgaaggtcc ggacgcagtt cgaccgcccc
     3601 atcggttcgt tccaggcggt caagcatcag ttggcggacg ctcttctgcg ggtcgaatca
     3661 tctcgttcgg ccgcgctcta cgcggcatgg gccaactccg tcggggcccc cgatgcgaag
     3721 ttcgcctcgt ccgtcgccaa ggcctacatc gccgaggctg cggtctgggt cacgggggcc
     3781 ggcgtccagc tccacggcgg catcggcttc acctgggagc acgactcgca tctctacttc
     3841 aaacgggccg tcactaacgc cgctctcctt ggcgacgccg cccaccaccg cgcccgcgcc
     3901 ctctccctct ccccgttctt gggcgaataa ccaccgctag gcggtggtta ttcgcccaag
     3961 ttcgaagtgt caggggtcat gtgtatacat ggaccatgga tctgttcacg ctggcggccc
     4021 agcagtacgg cgtcatcacg cggaggcagg ccctcgaacg gggcatgtcc cctagcgccc
     4081 tcgataggtc actcgatcgc ggcgaatgga tcccattggc tcccggcgtc taccgccacg
     4141 catgttctcc gccctcttgg cgccaggaac tcgcagcggc gcacctcgct atgggttggc
     4201 cctccgctgt ttcgcaccgc tcagcggcag cgctatggaa gctcagtggt ttcggtgaag
     4261 acatggtcga gctcacttgc atcctggggt cggctcacac ccccgagccg cgctggatgg
     4321 ttcatcgcac taattgggtt gagtcgcaga tcacccagat cgacgggatc ccagttacga
     4381 atgtcgagcg gacgctgatg gatctatgtg ccgtgatacc tctgggccga gtcgcaagag
     4441 gcctcgatga agccctcatg cgcggcttga cttcggttga gtcgatacgg gggtggctcg
     4501 gggaggttgg gggccgcggt cgaaggggag ctggcatcct taggaatctc gtcgacgagc
     4561 gagctccgat gccccggggt cttgagagtg tcctcgaggt gaaagtcatg agaatcatcc
     4621 gaagcgcggg tcttcctctt cccactccgc aatacgaact acgtgacggg agtcgagtgc
     4681 tagcccggct ggattttgca tatccgagta gaaaattcgc catcgaagca gacggcttca
     4741 agtatcacgg gggccggaca gcttggcgta aggacctcga tcgtaacaac gttatcgcag
     4801 cgatgggttg gcaggtgcta catgtcacct gggacgatct gcgcgacagg agggcaaagt
     4861 tcatcagcga cgtaacagcg ttcttgggcg agaaaccacc ggtaggcggt ggtttctcgc
     4921 ccaagttcga gaggggttag aggtagcctt ctttcggctt cgcctcggcg gcctcggcgg
     4981 cctcggcggt ggacttctcg gccaattcga ggacgtggga ctgcacgctc gcctgcagac
     5041 cttggaagag gcccccgagc caaccgatga ggcccgagta agcgaccaga acctcttgct
     5101 gtgtggcgcc atctgcgaag ggaaggtcca gttggatcga ttcgagctcg tcgatcaact
     5161 ccttggggag agccgactcc aactgcttct tgactttgag gtataggccg gcgaactgtt
     5221 cggcgggcgt agtggccggg tccatcttgc ggagttcctc gagcacctcg cgtgcgaggc
     5281 tcgcgatgcg caataacttg ggggggtcga agacccctcc atcagctttg gtttctacgc
     5341 tctcgtcatc catgcctgga atcataagca ttccgatatc ctctggttat ccaaggcaat
     5401 cgatagggag gccgccgtgc ccgatgctgt gatcgtggat ggaacaagaa gcccgatagg
     5461 gcgcgcctat aagggcgcgc tcaaggacgt ccgcccagat gatctggggg gctacatcgt
     5521 tcagaagttg atggagaagt tcccggatct tgacccgacc actatcgatg acgtgatggt
     5581 cgggtgcggt ctgccccacg gcgaatccgg cttcaacgta gcaaggccga tcgttctact
     5641 ttcggggctc cccaacagcg tcccggccac gacggtgaac cgtttctgct cttcgagcct
     5701 tcagacgatc cgcatggcgt tccacgccat caaggcggga gagggttcta ccttcatcag
     5761 tgccggcgtc gagtcgtcgt cgcgagtcga actactgcca aggggcgatg taaccgttct
     5821 gaacccccga ctttcgcccc tgttcggtgg tgagtacatc gccgacatct acatctcgat
     5881 gggcgagacc gcagagaacg ttgccgatcg cttcgatgta tcgcgcgagg acatggaccg
     5941 attcgcacag caatcacaag agcgcgcggt gtcggcccag aaggatgggt tcttcacgaa
     6001 ggagatcact ccgatcccgc tcgcaacgtc cggggagcgc accatcatcc aagagggagg
     6061 tgactccctg ctggtggagc atgacgacgg cccccgcccc tcttcgactt acgagaaact
     6121 cgcatcgctg gaaggggcct tccgtcctgg cggcaaagtg accgcgggta actcctgccc
     6181 cctcaacgac ggagctgccg cagtgctcgt tatggaagag gcgaaggcga ggagtttggg
     6241 cctgaagccc cgtgtccgta tcatcgcgag tgctgtaagt gcgctcgagc ccgagatcat
     6301 gggggtcggg ccgatcgagg ccgttcgaaa ggctctcgcg atcgcgggca tgaccatcga
     6361 agacatcgat gtcgtagaac tcaacgaagc cttcgccgcg caagttctgc ccgtctgtcg
     6421 tgagttgggg atagacccat tctcggacaa actcaatcca cacggtggtg cgatcgccct
     6481 cggacacccg ttcggcatga ccggttcgcg catcatgaac actctgatga acgaccttga
     6541 gaccctcgac cggacaatcg gactcgagac gatgtgtgtc ggtggcggtc agggtatggc
     6601 gatgatcgta gagaggatca actgatgacc acgatcatca aaggcaagca ggagttgctc
     6661 ggccgggttg gcgacgagct cggtgtctcc gaatggcatg agatcaccca ggatcaggtc
     6721 aacaagttcg ccgacgcgac aggcgatcac caatggatcc acgtcgaccc cgagaaggcc
     6781 gccctcggcc ccttcggaac aacgatcgcg catgggtatc tgaccctcgc gctgggaccg
     6841 aaactctcgt gggagacggt caccgtagag ggcgtccaga tggggatcaa ctacggatcc
     6901 gaccgtgttc gtttcatcac gcccgtgaag gtgggagccc gcataaggat gcggatcaag
     6961 ctcgctgacc tcaaggaggt cgagccaaac ggcctccaag cgacattcga gaacaccttc
     7021 gagatcgagg gagaagataa gcctgcttgt gtcgcccagg taatctcgcg ctactacttc
     7081 tgaaatcatc gccataatgt ctgagcgatg atgcacggcg cgggtttccg gatgatcaat
     7141 ccggggcgag acgacaccaa ggacgttcag ctctcgaagg ggctggttcg ccgcgtcttc
     7201 aagttcgcta agccttatcg aggctcgatc gtcctggcca tgatcgtcat cctggcggca
     7261 gccgtggcca cgggtctcgg acctcagatc atccgcagga tcatcgacga ggccatcacc
     7321 caccgtgaca aggcgaagct caaccttttg atagcactgt ttcttgggct tgccctggcg
     7381 ggatcattcc tggggatcgc aatgaggtat ctgctttcac ggatcggtga gggcctgatc
     7441 ttcgatctcc gttcttcgtt gttcgaccat gtccagcgga tgccagttgc cttcttcacg
     7501 cgcacgcaga cgggtgcttt gataagcagg ctcaacaacg atgttatcgg cgctcagcgt
     7561 gctgtaaccg agactacggc gggtctcctc caggtaatcg ccgaccttgt ggttaccttg
     7621 gcgctcatgt ggtcgatgca accgaggctg accgtaatct cgctcctcgt ggtccccctc
     7681 ttcgttcttc cgattaggag catggggaag gtgctgcagc gatacgtgag gcagcagatg
     7741 gagttcaacg cttcgatgaa cacgcagatg acagaacggt tccagatcgg aggagcactt
     7801 ctcgtcaagc tcttcggcaa ctacgtgaaa gagcgggatc acttcgcaca gaaggctggc
     7861 aacgttcggg atctcggcgt gaagaccgca gtcatcgggc gtctattctt cgtcacgttc
     7921 tctgtcgtct cggcctttgg cacggccatg acctactggc tcggcgggcg gatggcgatc
     7981 tccggcgaga tcaaggtcgg agtcatcgtt gccttctctc tttaccttgg tcgcctctat
     8041 ggacccatca cgggcctcag caacacgccg gtcgaggtca agaccgcgtt ggtttcgttc
     8101 gaccgagtct tcgaggtcct cgacttcccc agcgcgatcc agcagaagcc tgacgcggtc
     8161 gaactaaccg accctcgcgg aaaggtcgaa ttcgtagggg tgaggttccg ctacccgccc
     8221 ggttcggaga cgtcgatacc ctccctggaa gagggtcgca tcgatacgaa gtacgacccc
     8281 gaggcttggg ttctcaaaga tgtctcgttc accatcgagc cagggcagat gttcgcgctg
     8341 gttgggccct cgggggccgg gaagaccacg atctcgatgc tcgtaccccg tctctacgat
     8401 gcggtcgatg ggacggtcct catagatggc atcgacgtca aagacttgac cctgccgagt
     8461 ctcgcagcgt cggttggtgt ggttacccag gactcccaca tgttccacga caccatcaga
     8521 gccaacctc
//
LOCUS       DATDOZ010000113         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1918494, whole genome shotgun sequence.
ACCESSION   DATDOZ010000113 DATDOZ010000000
VERSION     DATDOZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918494"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VI541_02160"
     CDS             <1..189
                     /locus_tag="VI541_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLF69741.1"
                     /translation="PDPPIYTHTCRELGLEPGRCVFVDDLLANVEGAQAVGMKGIHHT
                     SPGETLGLLQTFFGLDLA"
     gene            227..1516
                     /gene="radA"
                     /locus_tag="VI541_02165"
     CDS             227..1516
                     /gene="radA"
                     /locus_tag="VI541_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLF69742.1"
                     /translation="MSKTALACIQCGYRTLQWVGRCPSCGAWESFVDGSVDQVAPPIN
                     LADITCDDHNRITSGIGELDRVLGGGLVAGSVVLLAGEPGIGKSTLMLQAAASMQKMG
                     RSVLLVCGEESLEQVAARAVRIGGVAASAHSATDTNVICVLMRSFEVVIVDSVQTLRE
                     PAASGEPGSVTQVRSSAMALSRAARESGAAVVLIGHVTKDGAVAGPRVLEHLVDAVIT
                     FEGDGGHSLRVVRSVKNRFGPSGEVGIFEMGATGLKEIPDASGVSLGPRRDVSGYAVG
                     CAIEGRRPLAIEIQSLAIESKAQLTRRVTHGIDPSRLGVLLAILQRRAGVALATFDVY
                     ATVAGGLRISDPGLDLAIALAAAASRRDRTITKGVAAVAEIGLGGELRPVPGMEPRLR
                     ELTRLGFDRVIVPCETQGKDPALHRVATLREAIEVAL"
     gene            1668..2159
                     /locus_tag="VI541_02170"
     CDS             1668..2159
                     /locus_tag="VI541_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLF69743.1"
                     /translation="MPATTSFRVGDKVVYPHHGAAIIKSLEEKEFQGETKQYFILRLA
                     YGDLTLMVPADNTEEVGIRDVIARDELDKVFKVLKSKECRMPANWSRRFKTHVEKLRS
                     GDIYQVAEVVRNLSRREAEKGLSAGEKRMLTKARQILVSEVTFAANVDEAEAEKMLDK
                     VLA"
     gene            2214..>2564
                     /locus_tag="VI541_02175"
     CDS             2214..>2564
                     /locus_tag="VI541_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HLF69744.1"
                     /translation="MARPVVVAILLAGGSGDRADPERVSGGDAKQFREVGGAPMWEHS
                     LATFERCDSIDSVIVVVSQDAPAPDPSFHKIASVTLGGPTRQASLGAGLACLPGGVDI
                     VVVHDAARPVISASL"
BASE COUNT          436 a          794 c          833 g          501 t
ORIGIN      
        1 cccgatccgc cgatctatac gcacacctgt cgtgaactgg ggctcgaacc cggacgctgc
       61 gtgttcgtcg acgatctgct cgcaaatgtc gagggggcgc aggccgtggg tatgaagggc
      121 atccaccaca cttcgccagg ggagaccctg gggctgctcc agactttttt tggcctcgac
      181 ctcgcctgag ccccgaactg tcagaggcgg tctatagatt gctggcatgt ccaagacggc
      241 tcttgcatgt atccagtgcg gctatcggac cctccagtgg gtcggccgct gcccatcgtg
      301 tggggcatgg gagtccttcg tcgatgggag tgtcgatcag gtcgctccgc ccatcaacct
      361 ggctgacatc acatgcgatg accacaaccg gatcacatca ggcatcgggg aactggatcg
      421 ggtcttaggc gggggcctcg ttgcgggttc ggtggtgctg ctggctggcg aaccaggcat
      481 aggtaagtcg acgctgatgc tccaggccgc tgcgtccatg cagaagatgg ggcgaagtgt
      541 actgctcgtc tgtggggagg agtctctcga gcaggtggct gccagagccg tgcgcatcgg
      601 gggtgtcgca gcgtctgccc actccgccac ggacacgaac gtcatctgtg tcctgatgcg
      661 ctctttcgaa gtggtcatcg tcgactccgt tcagacgctc cgcgaaccgg ccgcgagcgg
      721 cgagccgggc tcagtgacac aggtgcgctc atcggccatg gcactctcga gagcggctcg
      781 cgagagcggt gccgctgtag tgctcatcgg tcacgtcacc aaggatggcg ctgtcgccgg
      841 cccccgcgtg ctcgaacatc tcgtcgacgc ggtcatcacc ttcgaaggag atgggggcca
      901 ctcactgagg gttgttcgga gcgtcaagaa ccggttcggc ccctccggtg aggtcggtat
      961 cttcgagatg ggagcaactg gcctaaagga gatccctgat gcctccggtg tgtcactggg
     1021 tccacgtcgc gacgtctcgg gctacgcggt cgggtgcgcg atcgaggggc ggcgtccgct
     1081 cgctatcgag atccagagtc tcgctatcga gagcaaggca caactcacgc gccgcgtgac
     1141 tcacggtatc gatccatccc ggctgggggt cctcctggcc atcctccagc gccgcgctgg
     1201 ggttgctctg gccacattcg atgtctatgc cacggtcgcc ggtggtcttc gcataagcga
     1261 cccaggcctc gatctcgcga tcgccctcgc cgcagcggct tctcgtcgag atcggacgat
     1321 caccaaggga gtggccgctg tcgccgagat cgggttaggg ggcgagttgc gaccggttcc
     1381 aggcatggag ccgcgtctgc gggagttgac gcgcctcggg ttcgatcgtg tgatcgtgcc
     1441 gtgcgagacc caggggaagg accctgcgct ccaccgcgtg gcgacactgc gcgaggcgat
     1501 agaggtggcg ttatgagcca ttcgcccggt ctgtcataca tcgccctttc ttgtggcccc
     1561 ctcgtcggcc tgttaccatt gaagtctcgc ccccgatcca gttcggggcc cgacttcgaa
     1621 caatgccggg ccctcgcata gggccttcac gactgaagga ggatcgtttg cccgcgacga
     1681 ccagttttcg tgtaggtgac aaggtcgtat atccccacca cggggcagca atcatcaaga
     1741 gtttggagga gaaggagttc caaggcgaga ctaagcagta cttcatcctt cggctggcat
     1801 acggtgacct gacgctcatg gtcccagcgg acaacaccga agaggtcggg atccgcgatg
     1861 tcatcgctcg cgatgagctc gacaaggtct tcaaggtgct caagtccaag gagtgccgga
     1921 tgcctgcgaa ctggagccgt cgcttcaaga cccacgtcga gaagctccgt tctggtgata
     1981 tctaccaggt ggccgaggta gtgcgcaacc tctcccggcg cgaggccgaa aagggtctct
     2041 cagccggcga gaagaggatg ctcaccaagg cccgccagat cctggtctcc gaggtgacct
     2101 tcgccgccaa cgtcgatgag gccgaggcag agaagatgct cgacaaggtc ctcgcctaag
     2161 aagttaggcc ctactttccc cgacctcgcg gtagctctgg ggataatgcc ggtatggcac
     2221 gaccagtggt tgtggcgatc ctgctcgcgg gcggttcggg agaccgagcc gaccccgagc
     2281 gagtgtcggg cggtgatgcg aagcagttcc gtgaagtcgg cggggccccc atgtgggaac
     2341 acagcctcgc caccttcgag cggtgcgact ccatcgactc agtgatcgtt gttgtttcgc
     2401 aagacgcccc cgcccccgac ccgagtttcc acaagatcgc gtccgtcacg ctcgggggcc
     2461 ccactcggca ggcttcgctg ggcgccggtt tggcatgcct gccgggcggc gtcgatatcg
     2521 tcgtcgttca cgatgctgct cgtcctgtca tctctgcctc gctc
//
LOCUS       DATDOZ010000114         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1150810, whole genome shotgun sequence.
ACCESSION   DATDOZ010000114 DATDOZ010000000
VERSION     DATDOZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150810"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /gene="dnaK"
                     /locus_tag="VI541_02180"
     CDS             complement(<1..1648)
                     /gene="dnaK"
                     /locus_tag="VI541_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLF69745.1"
                     /translation="MPKVVGIDLGTTNSVVSALEGGDPVVIPNAEGHRTTPSIVAFSK
                     SGEILVGEVAKRQAITNPDRTIRSVKREMGTNWHTDIDGKKWTSQEISAQVLIKLKRD
                     AEAYLGDKVTQAVITVPAYFNDAQRQATKEAGEIAGLEVLRLVAEPTAASLAYGLDKE
                     GEQTILVFDLGGGTFDVSVLEIAEGVFDVKSTSGDSHLGGDDWDEVIIDHLLKEVLNN
                     HGVDLSKDRMARQRLKEAAEKAKIELSQTTQTTINLPFLTATDAGPIHVEVNLTRSQF
                     EQMTDHLLARVKGPFLQAVKDWGKPIDKINHVILVGGSTRMPMVVDLVRKLTGGKDPH
                     KGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSMGIETKGGIFTKLIERNTTIPT
                     RKSEIFSTADDNQPSVTVNVVQGEGQMVTSPGVRSLGQFDLVGIPPAPRGVPQVEVSF
                     DIDANGIVHVSAKDLGTGKEQKTTITGGTALPKDEIDRMMRDAEQFANEDQERREMAE
                     ARNMADNLIYQSEKSLKDFADKLTEEDRQAIETAVQTLKDA"
     gene            1940..>2610
                     /locus_tag="VI541_02185"
     CDS             1940..>2610
                     /locus_tag="VI541_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69746.1"
                     /translation="MFQKIVMTLVILTSLGLFSLRMIQLLEYLRLGRDENRFDNIGAR
                     ILHDLRVALGQQKILQWSFAGTMHVMIYWGFLVLGTTIVEAFGAVYQEGFALPFIGRW
                     GPLGAMQDIFVLAVLTGIAMAFYIRRVQKPGRFRGSHKKEAEYILFAITGIMVTILGL
                     RATEIALGHFPYPRQWTFASSFVASLTFDKLGLAAKHAWNTLFLWWHSLIILGFLVYL
                     GYSKHL"
BASE COUNT          430 a          779 c          790 g          611 t
ORIGIN      
        1 gtgcgtcctt cagcgtctgc actgctgtct cgatcgcttg gcggtcttct tcggtcagtt
       61 tgtcggcgaa atctttgagt gacttctcgc tctggtagat caggttgtcg gccatgttgc
      121 gagcctcagc catctcgcgg cgctcctgat cctcattggc gaactgctcg gcatctcgca
      181 tcatccggtc gatctcatct ttcggcagag ccgtgccacc tgtgatcgtc gtcttctgct
      241 ccttgccggt gccgagatcc ttggctgata cgtgcacgat gccgttggcg tcgatgtcga
      301 atgacacttc gacctgcggc acgcctcggg gggctggcgg gataccaacc aggtcgaact
      361 gaccgagtga acgcactcct ggcgatgtga ccatctggcc ctcgccctga acgacattca
      421 cggtcacgga aggctggttg tcgtcggccg tcgagaagat ctcactcttg cgagtcggga
      481 tcgttgtgtt gcgttcgatg agcttcgtga agatgccgcc tttggtctcg atgcccatcg
      541 acaggggagt gacgtcgagc aggagaacat ccttgacctc gcccttgaga actcccgctt
      601 gcagtgcagc gccaacggcc acgacctcat cggggttgac gcccttgtgg gggtccttgc
      661 ccccggtgag cttgcggacc aggtcgacga ccatgggcat ccgagtcgaa ccgccgacga
      721 ggatgacgtg gttgatcttg tcgatgggct tgccccaatc tttcacggcc tgcaggaagg
      781 gtcccttgac cctggcgaga agatggtcgg tcatctgctc gaactgtgac cgcgtgaggt
      841 tgacctcgac atggattggc cctgcatcgg tcgccgtaag gaacggcaga ttgatagtgg
      901 tctgggttgt ctgcgaaagc tcgatctttg ccttctcggc ggcttccttg agacgctgcc
      961 tggccatccg gtccttgctc aggtccacac cgtggttgtt gagcacctcc ttcaagaggt
     1021 ggtcgatgat cacctcgtcc cagtcgtccc cgccgaggtg actgtcaccg ctcgtcgact
     1081 taacgtcgaa gacgccttcg gcgatctcga ggaccgagac atcgaaggtg ccgccgccga
     1141 ggtcgaagac gaggatggtc tgctcaccct ctttgtcgag tccgtatgcg agagatgccg
     1201 cggttggttc ggccacgagt cgcagtactt cgaggccagc gatctcgccg gcttccttgg
     1261 tggcctgacg ttgggcatcg ttgaagtagg cggggaccgt gatgaccgcc tgagtgacct
     1321 tgtcgccgag ataggcctcg gcgtcgcgct tgagcttgat cagcacctgg gccgagatct
     1381 cctgcgaggt ccacttcttg ccgtcgatat cggtgtgcca attcgtgccc atctcgcgtt
     1441 tgaccgaacg gatcgtccgg tcggggttgg tgatggcctg gcgcttggcg acctctccga
     1501 cgaggatctc ccccgacttc gagaaggcca ctatcgatgg ggtggttcgg tgcccctcgg
     1561 cattggggat gaccacgggg tccccgccct cgagtgcgct tactacagag ttggtggtac
     1621 cgaggtcgat acccactacc tttggcatgt tctagccttt ctcgatgctc cttcggagcg
     1681 tgatttcctc acctgcgggg taggaggtta ggctcccaac cttgaggctc ttggaatagt
     1741 aaaacttgag tgcttgtctg tcaagttcgg cctctatcgg ctgaggctgg agccgtattc
     1801 ggctctgttg gcggtctaat gcaaacaacc accgctgccg tgctcatttg caataagagt
     1861 gcacaagggc cgacccgctg gccagatcgg tggcccttac tcgtacaatg ccccctgcgt
     1921 ccacacctaa ggagcagatg tgtttcagaa gatagtcatg acgctggtga tcctcacgtc
     1981 gctcggcctc ttctcgctgc ggatgatcca actcctcgag taccttcgcc tgggcaggga
     2041 cgagaaccga ttcgacaaca tcggggcccg gatcttgcac gaccttcgag tcgcactggg
     2101 tcagcagaag atcctgcagt ggagcttcgc tggcacgatg cacgtgatga tctactgggg
     2161 gttcctcgta ctcggaacca cgatcgtcga ggccttcggt gcggtctatc aggaggggtt
     2221 cgcgctccca ttcatcgggc gttggggccc cctgggcgcg atgcaggaca tcttcgtgct
     2281 cgcagtcctg acaggtatcg cgatggcctt ctatatccgg cgcgtccaga agcccgggag
     2341 gttccggggc tcccacaaga aggaggccga gtacatcctc ttcgcgatca cgggcatcat
     2401 ggtcacgatc ctcgggctgc gagcgaccga gatcgcgcta ggacacttcc cgtatccgcg
     2461 ccagtggacc tttgcgtctt cgttcgtcgc gtcactgact ttcgacaagc ttggcctcgc
     2521 cgcgaagcat gcatggaaca cgctcttctt gtggtggcac tcgctgatca tcctggggtt
     2581 cctcgtttac ctcggatact cgaagcatct
//
LOCUS       DATDOZ010000115         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_357204, whole genome shotgun sequence.
ACCESSION   DATDOZ010000115 DATDOZ010000000
VERSION     DATDOZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357204"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VI541_02190"
     CDS             <1..812
                     /locus_tag="VI541_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLF69747.1"
                     /translation="ALVIDDFPPRREPPPLPAAPVDTSLRQGSTGAEVLSLEQRLSAL
                     GYLVGKVDGIFDRSTYFGVVALQKVEGLGRTGRVDGPTIGRLATAYRPIPRYTTPPDH
                     LEVDIARQVVLVVRAGVVSEILPTSTGSNKLFTNEGWTRRATTPNGRFKVTRKIPKMR
                     ISPLGELYKPSYFNGGIAFHGNGSVPPYPASHGCVRLPMPFADWFFNNASPIGMVVYV
                     YGGPAGDNPQPVIEDRPAEVEPSPSPSPSPSPSPEPSPSETSPTTTVTVDP"
     gene            complement(820..1692)
                     /locus_tag="VI541_02195"
     CDS             complement(820..1692)
                     /locus_tag="VI541_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLF69748.1"
                     /translation="MSVRWSELRSRAWNRWRGPTAFVLSGGGPAGAAQVGMLQGLLEA
                     GIVPDAMIGTSVGALNAVFIANDPTLRGAEKLREVWMSMRKEDLFPGGKISSALNILR
                     RGSCVFSNSGLRGIIESGLSASTFEQLKIPAHINATQLSNGEERWFSSGSIADALLAS
                     AAMPGVFPPVQIDGHAYIDGGVTNNVPVLRGAEIGAKNVIVISVNAASQRRELSRPHD
                     FMMHGFVLARGHRYERDLAAARQKACVSVIPTLELGYVAFTDMSQTERLIEAGLAATR
                     KWLSEGPQSRRHRK"
     gene            complement(1799..2035)
                     /locus_tag="VI541_02200"
     CDS             complement(1799..2035)
                     /locus_tag="VI541_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69749.1"
                     /translation="MSGLRDPQGKAALFSGAPLSAGRWVVECSDCEAITRLDTLGLLR
                     GVLPLSVTIPLRRHTTWMRCPACGRQRWVKISLR"
     gene            complement(2032..2778)
                     /locus_tag="VI541_02205"
     CDS             complement(2032..2778)
                     /locus_tag="VI541_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF69750.1"
                     /translation="MSPTFALANQKGGVAKTTSVASLGTALADDGYRVLLVDLDPQGS
                     LTYSLGVDPDTLDLTIHDVLLGRMPLRKVIIQTGSGDLVPSNIELAGAELFLFSKTGR
                     EYELSNILEEVKREYDAIFIDCPPSLGVLTINGLTAADEVIIPFQCEALSQRGVAQLI
                     DTINDVRRYTNSNLKIRGILPTMFDPRTTHSREVLNNLATRYAIPVFDPPIRKSVRFA
                     EAPARGKSIVRLSPNHPGAIAYREIARSLI"
     gene            complement(2829..>3027)
                     /locus_tag="VI541_02210"
     CDS             complement(2829..>3027)
                     /locus_tag="VI541_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69751.1"
                     /translation="RFACDEEGAIHLVGQVPLACVDDEELDRLIGSVLEYQDENFKPF
                     LEIGFPLALEAIRQGKPIPEE"
BASE COUNT          575 a          961 c          925 g          566 t
ORIGIN      
        1 ccgctctggt gatcgatgat ttccctccac gacgagagcc cccgcctctg ccagcggccc
       61 ccgtcgatac gagtctgcgg cagggatcca cgggggccga agtgctctcc ctcgaacaga
      121 gactctcggc cctcggatac ctggtcggca aggtcgacgg tatcttcgac cgatcgacct
      181 acttcggtgt cgtcgcactt cagaaggtcg agggcttggg tcgaacgggt cgggtagacg
      241 gccccaccat cggccggttg gctaccgcat ataggccgat cccacggtac acgacacctc
      301 ccgatcatct ggaggtagat atcgctcgtc aggtagtgct tgttgttcga gccggcgtgg
      361 tgagcgagat acttccgaca tcgaccggat ccaacaagtt gttcacgaac gaggggtgga
      421 cccgaagagc cacaacgccc aacggacgct tcaaggtcac tcgcaagatc ccgaagatgc
      481 ggatctcacc cctcggcgaa ctgtacaagc cctcctactt caacggaggt atcgctttcc
      541 atggcaacgg ctccgttccg ccctaccccg cgtcacacgg atgcgttcgg ctaccgatgc
      601 cgttcgcaga ctggttcttc aacaacgcct caccgatcgg aatggtggtc tatgtatatg
      661 gagggcctgc gggcgataac ccacagccgg tgatcgagga ccggcctgca gaagtcgagc
      721 cttcgccgag cccatcaccc agtccgtcgc cttccccgga gccgagccca tccgagactt
      781 cgccgaccac cacggtcact gtcgatccgt aggacgcacc tacttgcgat gacgcctgct
      841 ttggggcccc tctgaaagcc acttcctcgt cgctgcgagg cccgcttcga tgagtctctc
      901 ggtctgtgac atgtcggtga aggcaacgta tccaagctcc aacgtcggta tgacgctgac
      961 acatgccttc tggcgcgctg ccgccagatc tctctcgtag cggtgccccc tggcgaggac
     1021 gaagccgtgc atcatgaagt cgtgaggacg gctgagttca cgccgctgtg aagcagcatt
     1081 gacactgatc acgatcacgt tcttcgcgcc gatctcggcc cccctcagca ccggcacgtt
     1141 gttcgtaacc ccaccgtcga tgtacgcgtg cccatcgatc tgcaccggcg ggaacacccc
     1201 tggcatggcc gccgaggcca gcagggcatc ggcgatcgaa ccgctcgaga accaacgctc
     1261 ctcgccgttc gaaagctgcg tcgcgttgat atgagccggg atcttcagct gctcgaatgt
     1321 cgaggcgctg agccccgact cgatgatccc gcgaagcccc gagttcgaga atacgcagga
     1381 gccgcgccgc aggatgttca gtgccgaaga gatcttcccg ccggggaaga ggtcctcctt
     1441 gcgcatcgac atccaaacct cgcggagctt ctcggccccc cgaagtgttg ggtcattggc
     1501 gatgaagacg gcgttgaggg ccccgacaga tgtgccgatc atggcatcgg ggacgatccc
     1561 cgcttcgagc agcccctgga gcatcccgac ctgggctgcg ccagcgggac ccccgcctga
     1621 taggacgaaa gccgtagggc ctctccacct gttccaggcc ctcgatcgaa gctcactcca
     1681 ccgaacagac atgccgatcc ccccctgctt ctacctatct atcgagtgag ggggccggac
     1741 gttacggtat ctgcggaact tcctacccgt tgagcgccgg gcatgggcct tgggcggatc
     1801 agcgaagcga gatcttcacc catcgctggc gaccgcaggc tgggcagcgc atccaggtgg
     1861 tgtgtcggcg caaggggatc gtgacactga gcgggaggac gccgcggagc aggccgagtg
     1921 tgtcgagccg ggtgatcgcc tcgcagtcgg agcactcaac aacccatcgt ccagcggaca
     1981 gtggagcccc cgagaagagc gcggccttgc cctgaggatc gcgaaggccg ctcatatgag
     2041 gctccttgcg atctcacgat acgcgatcgc tcctggatga ttcggcgaga gacggacgat
     2101 cgactttccg cgcgcggggg cctcggcgaa ccgcaccgac ttgcggatcg gcggatcgaa
     2161 tacggggatg gcgtagcgcg tcgcgagatt gttcaagacc tctcgcgaat gagtggtcct
     2221 cggatcgaac atcgtgggca ggatcccgcg gatcttcagg ttcgagttgg tgtatcggcg
     2281 cacatcgttg atggtgtcga tcaactgcgc gacgccgcgc tgcgatagcg cttcgcattg
     2341 gaatgggatg atcacctcgt ctgccgcggt gaggccgttg atcgtcagaa ctcccagaga
     2401 tggcggacag tcgatgaaga tggcgtcgta ctcgcgcttc acctcttcga ggatattact
     2461 cagttcgtac tctctccccg tcttcgagaa gaggaacaac tcggcccccg cgagttcgat
     2521 gttggatggt acgagatcac ccgaccccgt ctggatgatg accttgcgca gcggcatacg
     2581 gccgagaagg acatcgtgga tcgttaggtc gagcgtatcg ggatcgacac cgagcgagta
     2641 ggtcagagaa ccttgagggt caaggtcaac gagcagcacg cgatagccgt catcggccag
     2701 cgccgttccg agagaagcga ccgaggtcgt cttagccacg ccacccttct ggttggcgag
     2761 tgcgaaagtt ggcgacatgg cgacacttta cttccatcac ggcggatggg gccctgaaga
     2821 tcctagggct actcctccgg gatgggtttg ccctgccgga tagcctccag ggccaggggg
     2881 aagccgatct cgaggaaggg cttgaagttc tcgtcctggt actcgagcac gctccctatg
     2941 agtcggtcga gctcttcgtc atcgacgcat gccagcggca cctgaccgac gaggtggatg
     3001 gccccctcct cgtcgcatgc gaaacgc
//
LOCUS       DATDOZ010000116         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_880018, whole genome shotgun sequence.
ACCESSION   DATDOZ010000116 DATDOZ010000000
VERSION     DATDOZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880018"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VI541_02215"
     CDS             <1..479
                     /locus_tag="VI541_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HLF69752.1"
                     /translation="AGVNRAKGLICAVDSDATNVYIALLARSMNPDLFIVARAAEPGS
                     ADRLYRAGADRVISPYVSSGRHMAMLSLRPKVVDYLEVDAGTRSPLTVEEMLIDRNSP
                     LVNRPIVESCGLASVLAIRQADGRIVANPSVSTTLREGDLILLLGEREVLRPVDSH"
     gene            complement(476..1144)
                     /locus_tag="VI541_02220"
     CDS             complement(476..1144)
                     /locus_tag="VI541_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF69753.1"
                     /translation="MADSVRVMLVDDHEVVRKGIRALIEVEDGMEVVGEAANGAEALR
                     NVPLCHPDVVVMDVRMPDGGGIEACRALRDAHPDVQVIMLTSYSDDEALFNSIMAGAA
                     GYILKQIRGRDLVDGIRTVASGHSLLDPGVTKRVLERLKRAKVEDQDPGLARLSPTEA
                     RILDMIAEGSTNKGIAAQINLSEKTVKNYVSSILQKLSVARRSEAAAYVVRAHSRETR
                     SDND"
     gene            complement(1146..>2367)
                     /locus_tag="VI541_02225"
     CDS             complement(1146..>2367)
                     /locus_tag="VI541_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLF69754.1"
                     /translation="QIAQLFASQAGVAIENARLYAQVTERSESLARKLAQLSSVDLIG
                     RLIITEEGVNRILRSVVEESMALTHSRSSILMLLDDDTGDLVARVARGALAAAVDLRI
                     PNGTSKAHGVMERMRGEAVEDLSADPEVHDETWRKLLKPRRGAFAPLVVRGGSVGVLA
                     VYDPSGGGSYSDDEVNVLQMLANQAAIALENERMTEALRVLAILEERERISKELHDGV
                     IQAIYSVGLSLQGTAQIIGRDPIVAGERIESAIGELDEVVRDLRSYIFHLRPRSVEER
                     GLESAIRELARDLEVNTIAHVEVQLDAGAGAGLPEVDQIHVIQIVREILSNIARHSGA
                     SEVEITMTADSDHILLRIDDDGVAFEPDRVVAGHGLRNVDERARALGGELQIVGRNPK
                     GMRHAVDIPIRRGQ"
BASE COUNT          436 a          771 c          664 g          496 t
ORIGIN      
        1 ctgctggcgt caaccgagcc aagggcctga tatgcgcggt cgactctgat gcgaccaatg
       61 tctacatcgc gctacttgcg cgctccatga accccgacct cttcatcgtt gccagagccg
      121 cagaaccagg ctcggctgat cgcctctacc gcgccggggc cgatcgtgtc atctcgccat
      181 atgtgagcag cggtcggcac atggccatgc tctcgttgcg ccccaaggtc gtggactatc
      241 tcgaggtcga tgccggcacg cgttcccccc tcaccgttga ggagatgctc atcgacagga
      301 actcaccgct tgtgaaccgc ccgatcgtgg agagctgcgg cttggcctct gtcctcgcca
      361 tccggcaggc ggacggtcgc atcgtcgcca acccttcagt aagcacgaca ctcagagagg
      421 gcgatctcat cctgttgctc ggcgagcgcg aggtgctgcg ccccgtggat agccactagt
      481 cgttgtccga cctggtctcg cgggagtgtg ctcgcacgac atacgcggca gcctcggagc
      541 gacgggctac cgagagtttc tgaaggatcg aagagacgta gttcttgacg gtcttctcag
      601 acaggttgat ctgggccgcg atccccttat tggtgctccc ttctgcgatc atgtcgagga
      661 tccttgcttc ggtcggcgaa agcctcgcga gcccggggtc ctggtcctcg accttagccc
      721 gcttcaatct ctcaagaact cgcttcgtaa caccaggatc gaggagggag tgccccgagg
      781 ccactgttct tatcccatcg acgaggtctc gtcctctgat ctgcttgagg atgtatcccg
      841 cggcgccggc catgatcgag ttgaagaggg cctcgtcgtc agagtaggag gtcagcatga
      901 tcacctggac atcaggatgg gcatcgcgga gggcacggca ggcctcgatg cctcccccgt
      961 cgggcatccg gacatccatg acgacgacat ccgggtggca gagagggacg ttacgcaggg
     1021 cctcggcgcc gttagcagct tcgccaacga cctccatacc gtcctcgacc tcgatcagag
     1081 ctcttatgcc ctttcgtaca acctcgtgat cgtcgacgag catcacgcgt accgagtccg
     1141 ccatctcact gcccccttct gatcgggatg tcgaccgcgt gcctcatccc ctttgggttc
     1201 cgcccgacga tctgaagctc tccgcccaaa gcgcgggccc tctcatcaac atttcgaaga
     1261 ccatgtcccg cgaccactct atcgggctcg aacgcaacac catcgtcatc gatcctgaga
     1321 aggatgtggt ccgagtccgc ggtcatcgtt atctcgacct cacttgctcc cgagtgtcga
     1381 gcgatatttg agaggatctc ccggacgatc tggatgacat ggatctggtc aacctccgga
     1441 agacccgccc ctgcccccgc gtcgagttgg acctcgacat gagcgatcgt gttcacctcg
     1501 agatctcggg caagttcgcg gatcgcgctt tcgagtccgc gctcctccac agaacgcggc
     1561 ctcaggtgaa agatataact tcgcaggtca cgtaccactt catctaattc accgatcgcc
     1621 gactctatcc tctcgccagc cacgatcggg tctcgcccga tgatctgtgc cgttccctga
     1681 agtgagaggc ccaccgagta gatcgcctgg atgacgccat catgaagttc tttgctgatg
     1741 cgttcgcgtt cttcaaggat cgccagcacc cgcagtgcct cggtcattcg ttcattctca
     1801 agagctatcg ctgcctgatt ggcgagcatc tgaagaacat tgacctcgtc gtctgagtaa
     1861 gatcctccgc cactggggtc atatacggct agaaccccca cggatcctcc gcggacaacc
     1921 agtggagcga atgcaccacg ccggggcttc aggagcttgc gccatgtttc gtcatgcacc
     1981 tcgggatctg cggataggtc ctcgaccgcc tcgcccctca tcctctccat cactccgtga
     2041 gccttcgagg ttccgttggg tatccgaaga tccactgccg cggcgagggc cccgcgggct
     2101 accctggcga ctaggtcccc ggtatcgtcg tcgagaagca tgagtatcga tgaccgggag
     2161 tgcgtgagag ccatcgactc ctcgaccacc gaacgcagga tccggttcac cccctcttcg
     2221 gtgatgatca accgcccgat gagatcgacc gaggagagct gagcgagctt acgggccaaa
     2281 ctctcgctac gttcagttac ttgggcgtag aggcgagcgt tctcgatagc cacccctgcc
     2341 tgagatgcga agagttgggc gatctgt
//
LOCUS       DATDOZ010000117         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1924168, whole genome shotgun sequence.
ACCESSION   DATDOZ010000117 DATDOZ010000000
VERSION     DATDOZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924168"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VI541_02230"
     CDS             <1..102
                     /locus_tag="VI541_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69755.1"
                     /translation="EKQKAGKARMKQVGSVEVPQEAFMAALRLDDKR"
     gene            99..1280
                     /gene="hemW"
                     /locus_tag="VI541_02235"
     CDS             99..1280
                     /gene="hemW"
                     /locus_tag="VI541_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLF69756.1"
                     /translation="MNGNQGPPTSPGPRVARGVYVHMPFCVFRCGYCDFNAYSGMDEL
                     MSPYLDALLAQIRAEADGGRVDTIFFGGGTPTRSPTPHLARILEAIRDGFDVVPGAEV
                     TVEANPESVDARVFEQLLAAGFNRVSLGVQSTASHVLKSLGRVHDAQTATDALKGAVD
                     AGFGRVSADLIFGTPGETTEDLERSIDDVLAARVDHVSAYALTIEEGTPFAAMVERGR
                     MPSPDEDDQADRYELCMDRLERAGLQQYEISNWSGPGSWCAHNLGYWRGDDYLAFGAG
                     AHGHREGRRYWILRSPQAFIARSPDVEADHEIIPADGRVEEAAILGLRMVGGLDRALF
                     AKRWGVDPAHRWAQEFTALAAGGLVEVTDRYIRPTKRAMFLSSEIARSLLSTPLRSGA
                     I"
     gene            1369..2418
                     /gene="hrcA"
                     /locus_tag="VI541_02240"
     CDS             1369..2418
                     /gene="hrcA"
                     /locus_tag="VI541_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HLF69757.1"
                     /translation="MNLDKRKSAILRAIVRDYVRTGQPVGSQVLAQRYRLKVSAATIR
                     NDMGVLEELGYLSQPYTSAGRIPTDKGYRWFVDNWPGTAWPDLTGPQKAAIEGAIRPH
                     FADLHQTLESTSRLLSKLTEATAVATAPPPTSDRLRRIELFSRADGRVTLLLVADTGV
                     VEQSVVELSGKATEEDLEKLAKALNKELVGTELGELSSKLLASKSTAKPKPTARELQD
                     RSRIATEIDRIVGAGRDRIFRGGTANILSSDKFADIDVAHDVVGVLEQTPVISGLLEA
                     ARRSSAVVVFIGHELQIEQMQSCAVILAPYEVQGDRRGAVGVVGPTRMDYPHTISAVE
                     AVAAELSRMLDGLGD"
     gene            2422..3510
                     /gene="dnaJ"
                     /locus_tag="VI541_02245"
     CDS             2422..3510
                     /gene="dnaJ"
                     /locus_tag="VI541_02245"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLF69758.1"
                     /translation="MTDYYAVLGVARDASEDEIKKAYRALARKYHPDANPDDPQAEGK
                     FKELAEAFTVLSDEGRRRDYDRYGTAKPPAGGFDPFDLFASFFGGDPFGAFSRRGAAA
                     HGRDLAYEMTLTLSEVLTGVVRTVEVVAHFSCETCAGSGARPGSGAVACTRCSGSGQV
                     RSVQRSIFGDVMTSFVCPDCEGSGERIADPCETCRGEGRVRRSEQVEIEVPAGVDDQI
                     QIRRSGKGEAGRRGGPPGDLYVRFKVQPEAGYERFEDDLIFTTTIPFTQAVLGATKQV
                     QTLSGLIELEIPPGTPTSKQFKVRGKGVPHLQRGGAGDLYIKVEVEVPAALTPEQDAA
                     LREFARTRGEEVLEGQGLMRKLRSAFKS"
     gene            3507..>3652
                     /locus_tag="VI541_02250"
     CDS             3507..>3652
                     /locus_tag="VI541_02250"
                     /inference="COORDINATES: protein motif:HMM:NF042468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase PUA domain-containing
                     protein"
                     /protein_id="HLF69759.1"
                     /translation="MTHAPFFFVARADLELGHARLHGSDARHLAHVRRAREGQHVTLG
                     DGAG"
BASE COUNT          690 a         1118 c         1195 g          649 t
ORIGIN      
        1 gagaagcaga aggcaggaaa ggcacggatg aagcaggtcg gctccgtcga ggttccgcag
       61 gaggccttca tggcagccct tcgactggac gacaagcgat gaacggcaac caaggccccc
      121 cgacctcgcc cggcccccgg gtcgcacgcg gcgtctatgt acacatgcct ttctgcgttt
      181 tccgttgcgg ttactgtgac ttcaacgcct attcggggat ggacgagttg atgagtcctt
      241 acctcgatgc tctgctcgct cagatccggg ccgaggcgga tgggggccgc gtcgacacca
      301 tcttcttcgg gggaggaacg ccgacacgat ctcccactcc acatctcgct cggatcctcg
      361 aggcgatacg cgacgggttc gatgtggttc cgggagccga ggtaaccgtc gaggcgaacc
      421 ccgagagcgt cgacgcgcgc gtcttcgagc agctactcgc tgcaggcttc aaccgcgtga
      481 gcctcggagt tcaatccact gcgtcgcatg tgttgaagag tctcgggcga gtgcatgatg
      541 cccagactgc aacggacgcg ctgaagggcg ccgtcgatgc tgggttcgga cgcgtcagcg
      601 cggacctcat attcggaaca ccgggtgaga ccaccgagga tctcgaacgc agcatcgacg
      661 atgtcctcgc cgccagagtc gaccatgtat cggcctatgc actcaccatc gaggagggta
      721 cccccttcgc cgcgatggtc gagcgcgggc gcatgccctc gccagatgag gatgatcagg
      781 cggatcgcta cgaactctgt atggatcgtc ttgagcgcgc cggtctgcag cagtacgaga
      841 tctctaactg gtcagggccg ggttcgtggt gcgctcacaa cctcggctat tggaggggcg
      901 atgactacct ggccttcgga gcaggtgccc atgggcatag ggagggcaga aggtactgga
      961 tcttgcgctc cccccaggca ttcatcgcgc gctcccctga tgtcgaggct gatcacgaaa
     1021 tcatcccggc ggatggcagg gtcgaggagg ctgcgatcct ggggcttcgg atggttggag
     1081 gtctcgatag ggcactcttc gcgaagcggt ggggagtcga tccggcccac aggtgggctc
     1141 aggagttcac ggccctcgcg gctgggggcc tcgtcgaggt cactgaccga tatatccggc
     1201 caaccaaacg tgcgatgttc ctgtcgagcg agatcgcccg ctcgctcctc agcactccac
     1261 tccgttctgg cgcgatctag cgtcggtttc cgacggtcag ccgcgccaga acggaagtta
     1321 gcactcaact ggtgtgagtg ctagcctcag gggatggcag caaccgagat gaatctcgac
     1381 aaaaggaagt cggcgatcct gcgcgccatc gtgcgcgact acgttcgcac tggccagccc
     1441 gtcggctcac aggtcctggc tcagcgttac cgcctgaagg tctcggccgc cacgatccgc
     1501 aacgacatgg gggtgcttga agagttgggg tacctgagcc agccctacac ctcggcagga
     1561 cggatcccga ccgacaaggg ttaccgctgg ttcgtcgata actggccagg cacggcgtgg
     1621 cccgatctca ccggcccgca gaaggcagcg atcgaggggg ccatccggcc acacttcgcc
     1681 gacctgcacc agaccctcga gtcgacctcg cggctgctct cgaaactcac agaagccacc
     1741 gcggtggcca ccgcgccgcc tccgacttct gacaggttgc gaaggatcga gctcttctcg
     1801 cgggcggacg gcagggtgac acttctcctg gtcgccgaca ccggtgtggt cgaacagagt
     1861 gtggtcgagt tatcggggaa ggcaacggag gaggatctcg agaagctggc caaggctctg
     1921 aacaaagagc tggtgggcac ggagctcggt gagctctcgt cgaagttgct ggcgtcgaag
     1981 agcaccgcaa aacccaagcc aactgcaagg gaactgcagg atcgctcacg tatcgcgacc
     2041 gagatcgacc ggatcgtcgg ggcagggcga gatcgcatct tcaggggcgg caccgccaac
     2101 atcctctcct ccgacaagtt cgcggacata gatgtggccc acgatgtcgt aggggtcctc
     2161 gagcagactc cggtgatctc gggactacta gaggcggcac gccgttcctc ggctgtcgtc
     2221 gtattcatag gccacgaact gcagatcgaa cagatgcagt cctgcgccgt gatcctcgct
     2281 ccttacgagg ttcagggcga caggcgcggt gcggttggcg tggtaggccc aactcgcatg
     2341 gactatcccc acacgatctc ggcggtcgag gccgtggctg ccgaactgag ccggatgctc
     2401 gatggtttgg gtgactgaac gatgaccgac tactacgcgg tcctcggcgt ggcgcgagac
     2461 gcgtccgagg acgagatcaa gaaggcatat agggctcttg cgaggaagta ccaccccgac
     2521 gccaaccccg acgatccgca ggctgagggc aagttcaagg aactggccga agccttcacg
     2581 gtcctctccg acgaaggtcg tcgtcgcgac tacgaccgct atggaaccgc aaagccaccg
     2641 gctggaggct tcgacccatt cgacctcttc gcttcattct tcggcggcga ccccttcggt
     2701 gcgttctcgc gacgcggggc agcagcacac ggccgtgacc ttgcgtatga gatgacactc
     2761 acgctttcgg aggttctcac cggagtcgtt cgaacggtcg aggtcgtggc ccacttctcg
     2821 tgcgagacat gcgccggatc gggggctcga ccgggatcgg gggccgtggc ctgcacccgc
     2881 tgcagtggtt caggtcaggt gaggagcgtt cagcgaagta tcttcggaga tgtgatgacc
     2941 tcgttcgtct gccctgactg cgagggttcg ggcgagcgca tcgctgaccc ttgcgaaacc
     3001 tgccgtggcg agggccgggt ccgacgcagc gagcaggtcg agatcgaggt cccagcgggg
     3061 gttgatgatc agatccagat cagaagatcg ggcaagggtg aagcgggccg ccgcggcggg
     3121 ccccccggtg atctgtacgt ccgcttcaag gtccagccgg aggcgggcta cgaacggttc
     3181 gaggacgacc tgatcttcac cacaaccatc ccattcacgc aggcagtact cggcgccacc
     3241 aagcaggttc agacgctcag cggtctcatc gagttggaga taccaccggg gaccccaacc
     3301 tctaaacagt tcaaggttcg cggcaagggt gttccgcatc ttcagcgagg tggcgcgggc
     3361 gacctataca tcaaggtcga ggtcgaggtc cccgctgcgc taaccccaga acaggatgct
     3421 gcactgcggg agttcgcacg cacgcgcgga gaggaggtct tggagggtca gggcctgatg
     3481 cgcaagctcc gcagtgcgtt caagagttga cccacgcccc attcttcttc gtcgccaggg
     3541 ccgaccttga gttaggccat gctcgacttc acgggtccga cgctcgacac cttgcacatg
     3601 tacggcgcgc gcgcgagggc cagcatgtga ccttgggaga tggtgcgggc ga
//
LOCUS       DATDOZ010000118         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3768637, whole genome shotgun sequence.
ACCESSION   DATDOZ010000118 DATDOZ010000000
VERSION     DATDOZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768637"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VI541_02255"
     CDS             <1..243
                     /locus_tag="VI541_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69760.1"
                     /translation="DAERLAAEEANPETRNDLIVLDNYFIAYHRNVFEALSDRQLPES
                     DDELPTWFASAELGRHLLVHWRNVPLAPSSMPPQGR"
     gene            294..473
                     /locus_tag="VI541_02260"
     CDS             294..473
                     /locus_tag="VI541_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69761.1"
                     /translation="MAKLQFSPRQVDEMEFWEVAKILGVEETSEDPVYQRLKAYRGKG
                     KMPTARPIDVSTIGR"
     gene            474..>2279
                     /locus_tag="VI541_02265"
     CDS             474..>2279
                     /locus_tag="VI541_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69762.1"
                     /translation="MAGIVEQLELDTSSALRSIADIERSLASAASSFSSSISEAIEDA
                     AGKPVVLEGDATAVGTSIEDSVSSADTAVTVEDVESGQVTSSIEDSVSSADTLVVVEG
                     DASQLASEIESAAEGAAQGLAETIQAGLDSIDPSGIQDLGGALGEAGEESDGLANKLS
                     GLGVLTTAATGGAENLGKAVGKLGGASAVAGVGTVVALGAGLNKMAGEAEEAFLSQQR
                     FNATFGESARVIEQIDIGGLNTSLSELAVNLGSDDDAIRQAASDLGLLGDSAGATEGK
                     IAETVKTVIALTAQIVAANPALGSMEEVLPRVALALSGNARAGRSLGISFTEAQVTAE
                     ALASSGGKAKDQLTLFDKVAAGANLRSKQLGDTIANDVNKALDDSTIKLRAATARLGE
                     AREAFGETVRGPLAAVKTAFFETTTEIVKDAEFLGRGVSGALRSLVAGDIEIFLDEFG
                     LGARDVADAVVEIGKAGPKMQPAIDSFADLESAAANTPEILTRADQELLDFVATATGK
                     LPAVSAAFEAASSAGQSALDGLISKLPTVSSVFAEFASKTPVSIGAVTKALNQQVKDQ
                     EAFFVNIGTIISKGGVDIAQQYLDMGVEDAAALAKA"
BASE COUNT          488 a          646 c          724 g          421 t
ORIGIN      
        1 gacgccgaaa ggctggctgc cgaagaggcc aaccctgaga cccgcaacga tctgatcgtc
       61 ctggacaact acttcatcgc ctaccaccga aacgtcttcg aagccttgag cgacaggcag
      121 ttgccggagt cagacgacga actgcccacg tggttcgcct cggcggagct aggaaggcac
      181 ctgctggtgc actggcgcaa cgtcccttta gccccttcgt cgatgcctcc ccagggtcgg
      241 tagcttcggc gttgccactg ccgctgaatg agatcggacc cgtatatgca gagatggcga
      301 aactgcagtt ctcaccgagg caggtggacg agatggagtt ctgggaggta gccaagatcc
      361 tcggagtgga agaaacgagt gaggacccgg tgtatcaaag actgaaagcc taccgaggga
      421 agggcaagat gcctacagca agacccatag atgtttcgac gatagggcgc tagatggcag
      481 gcatagtcga acagctcgaa cttgacacca gttctgccct gcgctccatc gccgacatcg
      541 agcggagcct tgcttccgcc gcgtctagct tctcgtcgtc catatcggag gcgatcgagg
      601 atgcagcggg taaacctgtc gtcctagagg gtgacgccac tgccgtaggg acctccatcg
      661 aagactctgt ttcgtcggcc gataccgccg tgaccgtaga agacgtcgaa tcgggccagg
      721 tcacttcctc catcgaagac tccgtctctt cggcagacac tctggtagtc gtcgaggggg
      781 atgcttcgca actggcatcc gagatcgagt ctgccgccga aggtgctgct caaggactcg
      841 cagagacgat ccaggcaggc cttgactcca tcgacccctc ggggatacag gatctgggtg
      901 gtgcgctcgg cgaagcgggt gaggagtcgg atggtctcgc taacaaactg agcggcttgg
      961 gggttttgac tacagcagcg acgggtggag ccgagaacct gggcaaagcg gtagggaagt
     1021 tgggaggggc ctccgccgtc gctggtgtcg gaaccgtcgt ggcactcggc gcggggttga
     1081 acaagatggc aggcgaagca gaagaagcct tcctctctca gcagcgattc aacgccacgt
     1141 tcggcgagag cgccagagtg atagaacaga tcgatatcgg aggactgaac acgtctttgt
     1201 cggagctggc cgtgaatctt gggtcagatg acgacgctat ccggcaagcc gcgtccgacc
     1261 tcgggctcct cggggattct gccggagcga ccgaagggaa gatagccgaa acggtcaaga
     1321 ccgtcatcgc actgactgcc cagatcgtcg cagcgaaccc tgcgcttgga agcatggaag
     1381 aggttctgcc gcgtgtcgct ttggctctgt ctggaaacgc cagggctgga cggagtctcg
     1441 gcatctcctt caccgaagca caggtcacgg ccgaggcgct ggccagcagt gggggcaagg
     1501 ctaaggatca attgaccttg ttcgacaagg tagccgccgg cgccaacctt cggagcaagc
     1561 agctcggcga caccatcgcg aacgatgtga acaaggcact ggatgattcc acgatcaaac
     1621 tgcgagcggc gactgcgcgc ctcggagagg cgagagaagc gttcggggaa acggtcaggg
     1681 gacctctagc cgctgtgaag acagcgttct tcgagacaac gacagagatc gtcaaggatg
     1741 ctgagttcct cggtagaggc gtttcaggag ccctgcggtc tctcgtcgct ggagacatcg
     1801 aaatcttctt ggatgagttc gggctcgggg cacgcgacgt ggccgacgct gtcgtcgaga
     1861 tcggaaaggc tgggcctaag atgcagcctg ccatcgacag cttcgccgac ctcgaatccg
     1921 ccgctgccaa cacccccgag atactcactc gcgctgacca ggaattgctc gacttcgtcg
     1981 caacggctac gggaaaactc ccagcggtct ctgcggcgtt cgaggctgca tcttctgcgg
     2041 gtcagtccgc tcttgatggt ctgatatcca agctgcccac ggtgagttcg gtgttcgctg
     2101 agttcgcctc caagacgcct gtatcgatcg gcgctgtgac taaggcactc aatcagcagg
     2161 tcaaggatca ggaagcgttc ttcgtgaaca ttggaaccat catctctaag ggcggagtcg
     2221 atatcgcaca gcagtacctc gacatgggcg tggaagatgc agccgcgctg gcgaaggct
//
LOCUS       DATDOZ010000119         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_361399, whole genome shotgun sequence.
ACCESSION   DATDOZ010000119 DATDOZ010000000
VERSION     DATDOZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361399"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VI541_02270"
     CDS             complement(<1..1093)
                     /locus_tag="VI541_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLF69763.1"
                     /translation="MVNRRHLLLGAVALLLGASSLAFVATSLEGSRAPSYTVLARFHR
                     AGQLMKAGADVKLRGILVGRVASVQLRGDMVELRLAMDPAQFVPSDVSASIRGKTLFG
                     EKFVSLLDSDHSSAKLRDGDVVPEARTVDPFELEQVLDSAMPLLNEISPKDLGAALRA
                     LAEGFSGQEDVVRRTIDNGGSALTSLNDHTDALGSLLKGLPRSSDALARSAADLVSSA
                     EGLDRFNSTLVANEGKVRGALSDVPSWMTKLAEILGERRADVITLSVQGADILAVVSA
                     RRDILPATVGGLKHFTESWDTNLSVGCTMPDGTTIGQVHPELDGSTCWQIWQVDGEQQ
                     RVPGGYSPSERPTVLSDSPISDRAFKAQLR"
     gene            complement(1093..1959)
                     /locus_tag="VI541_02275"
     CDS             complement(1093..1959)
                     /locus_tag="VI541_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF69764.1"
                     /translation="MAQLKVERPLAAPLSFVRSRARALIYTVEGVGSQIEFFIKGIIG
                     IPAAATRFLGETLRLVGDITWGAGALLVGAGTAGVIFFMSFITGGLVGLEGFKGLQLI
                     GAEAFTGFVSAFANTREITPPIAGITLAAQVGAGYTAELGAMRINDEIDALEVMAMPT
                     IRYLVSTRILAALIAVTPLYLVSLFASYLATQLAVVNLYGLSGGTFSHYFHLFLPAKD
                     VAYSLIKVLTFAAAVALIHCYYGYRASGGPAGVGVSVGKAIRASIVTINILNLAMSAL
                     MWGIVDTVRIAG"
     gene            complement(1970..>2608)
                     /locus_tag="VI541_02280"
     CDS             complement(1970..>2608)
                     /locus_tag="VI541_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF69765.1"
                     /translation="VSIVPVILVSLAFGMVIALQVGNVARQLGAQALTGAAMVLAIVR
                     EAAPIATALLIAGAGGSAMTADLGSRTIRDEISAMEVLAVNPVHRLVVPRIWAAIFVA
                     VLLDGFVSVAGISGGYLFNVVVQGGTSGSYLASFGLLSQSADLYFALIKAAVFGLIAA
                     MVAVHMGLSAKGGPKGVGDAVNQGVVITFMLIFLANIIMTLLYFQVVPQKFT"
BASE COUNT          538 a          808 c          811 g          451 t
ORIGIN      
        1 ttcgcaactg agccttgaac gcgcggtcgc tgatcggcga gtcgctcaac actgtgggcc
       61 gttcgctggg cgagtaaccc cccggcactc gctgttgttc cccgtcgacc tgccagatct
      121 gccagcaggt gcttccatcg agttccggat gaacctgtcc gatcgtagtg ccgtcgggca
      181 tcgtgcatcc aacactcagg ttcgtgtccc aggactccgt aaagtgcttc agccccccta
      241 cggtcgcggg caggatgtcg cgcctggccg acacgacggc gaggatgtcg gccccctgca
      301 cggacagggt gatcacgtcg gctcggcgct caccgagtat ctccgcgagt ttggtcatcc
      361 agctcggaac atcgctgagg gcgccgcgca ccttcccttc gttcgccaca agcgtcgagt
      421 tgaatcgatc gagaccctct gccgatgaca ccaggtccgc ggcgctgcgt gcaagagcgt
      481 cactcgatct gggcaagccc ttcagcagcg atccgagggc gtcggtgtgg tcgttcaacg
      541 aagtcagggc gctgccgccg ttgtcgatgg tcctgcgcac gacatcctct tgtccggaga
      601 atccctcggc gagggcccgc aaagcggcgc cgagatcctt gggtgagatc tcgttgagca
      661 agggcatggc ggagtcgagg acctgttcga gctcgaaggg gtcgacggtt cgtgcttcgg
      721 ggacgacatc tccgtcccgc agtttcgcag acgaatgatc ggagtcgaga agtgaaacga
      781 acttctcgcc gaacagggtc tttcctcgga tggaggcgga gacatcagaa ggcacgaact
      841 gggccgggtc catcgctaac cgcagttcaa ccatgtcacc tcttagttga acagatgcca
      901 cgcgcccgac caggatcccg cgaagcttca cgtcggcccc cgctttcatc agctgtccgg
      961 cccgatggaa tcgtgcgaga actgtgtagg agggcgcccg actcccctct agggaggtgg
     1021 ccacgaaggc cagcgaactt gcgccaagta gaagagcgac ggcccccagc agcaggtgac
     1081 gccggttgac catcatcctg cgatgcgtac cgtatccacg atgccccaca tgagggccga
     1141 catggcgagg ttcaggatgt tgatcgtcac gatgctggcc cgtatggcct tcccgactga
     1201 taccccgacg cccgccgggc ccccactcgc gcggtagccg tagtaacagt ggatgagcgc
     1261 cacggctgca gcgaaggtca agaccttgat gagggagtag gcaacgtcct tggccggaag
     1321 gaacaggtgg aagtagtggc tgaaggtgcc accactcaga ccgtagaggt tgactaccgc
     1381 caactgggtt gcgaggtacg aggcgaacag agaaacgaga tagaggggag tcaccgcgat
     1441 gagcgcggcg aggatgcgcg tggaaaccag atatctgatg gtgggcatcg ccatgacttc
     1501 gagagcatcg atctcatcgt tgatgcgcat cgcccccagt tctgccgtgt aacctgcgcc
     1561 gacctgcgcg gccagcgtga tcccagcgat gggcggcgtt atctccctgg tgttggcgaa
     1621 cgctgatacg aatccggtga aggcctcggc tccgatgagc tgaagaccct tgaatccttc
     1681 gaggccaacg agcccacccg tgatgaagga catgaaaaag atcacgcctg cagtaccggc
     1741 gccgacgagt agcgctcccg ctccccacgt gatgtctccg acgagcctca aggtttcgcc
     1801 caagaaacga gtagcggccg caggaatgcc gatgatccct ttgatgaaaa actcgatctg
     1861 cgagccgacg ccctcgaccg tgtatatcaa ggcccgcgct cgagatctca cgaacgagag
     1921 gggggccgcc aggggccgtt ccaccttcag ctgagccatg tggtctccat catgtgaact
     1981 tctgtgggac gacttggaag tagaggaggg tcatgatgat gttcgcaaga aagatgagca
     2041 tgaaggtgat gaccacaccc tggttgactg cgtccccaac ccctttcggc ccccccttcg
     2101 cggagaggcc catgtggaca gcaaccatcg ccgcgatcag cccgaagact gccgccttga
     2161 tcagagcgaa gtagaggtcg gctgactggc tgagcaaacc gaaggacgcg aggtaggagc
     2221 ctgaggttcc accctggacg acgacattga ataggtaccc gcctgagatc ccggcaacgc
     2281 tcacgaatcc atcgagcaat acggctacga atatcgcggc ccagatacgc gggaccacga
     2341 gacggtgaac cgggttgacc gcgaggacct ccatggccga gatctcatct cggatagtgc
     2401 gcgaaccgag gtcggcggtc atggcggacc ctcctgcccc cgcgataagc agagcggtgg
     2461 cgatcggcgc cgcttctcgg acgatcgcga gcaccatcgc ggcacccgtc agggcctgcg
     2521 ccccgagctg tctcgccacg ttccccacct gaagggcgat caccatgccg aacgccaacg
     2581 agaccaagat gaccgggacg atcgaaac
//
LOCUS       DATDOZ010000120         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3255629, whole genome shotgun sequence.
ACCESSION   DATDOZ010000120 DATDOZ010000000
VERSION     DATDOZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255629"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VI541_02285"
     CDS             complement(<1..312)
                     /locus_tag="VI541_02285"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HLF69766.1"
                     /translation="MSRRVAVVVFPGSNCEQDAVHALRLLGAEPEYVFHRQTDLSDFE
                     AVVLPGGFSYGDYLRTGAIAAFSPIMDAVRTFAADGGPVIGICNGFQILCESGLLPGA
                     LR"
     gene            complement(309..572)
                     /gene="purS"
                     /locus_tag="VI541_02290"
     CDS             complement(309..572)
                     /gene="purS"
                     /locus_tag="VI541_02290"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HLF69767.1"
                     /translation="MRFSVAVEVTPKEGISDPQGLTIEQALPRLGFTGISELRAGKLF
                     TFIIEADSEAAAVARIEAACARMLANPVIEQFHIRTTVLEEAL"
     gene            complement(569..1459)
                     /locus_tag="VI541_02295"
     CDS             complement(569..1459)
                     /locus_tag="VI541_02295"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLF69768.1"
                     /translation="MTEALLNVDLPFIRSASGKVREVFDLADGRLLFVATDRISAYDV
                     VMSQGIPQKGRILTQLSRFWFDLMAEICPNHLAANDELPDAVSDDIRERSLVVDRLEM
                     LPVEFVIRGYLAGSGWKEYQTTGTVCEVKLPAGLRESDRLPEPIFTPATKATSGHDEN
                     ISEDMAADIIGGDVLKIARGYALDIYERASDHAATRGIILADTKFEFGILGGKVVLAD
                     EVLTPDSSRFWPADSWEPGRSQPSFDKQFLRDWLDSQGWDHSPPPPDLPADVIEGTQA
                     KYVEALERLTGEPVDAGGVV"
     gene            complement(1462..>2029)
                     /locus_tag="VI541_02300"
     CDS             complement(1462..>2029)
                     /locus_tag="VI541_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLF69769.1"
                     /translation="EVREVEEPFAEGEQKGSSAMPHKRNPIRTERLTGLARLVRAAVL
                     PAIENIALWHERDISHSSVERVSLPDTCILLDFMIAESTDLLLGLNVYPERMRENIEG
                     SYGLVFSQSVLLGLVDKGLTRDEAYRIVQEDAMRAWDSKAQLRDLLASDDNVAAVMSA
                     SDLDLCFQESRYLEHSQSIIDRLEDLER"
BASE COUNT          404 a          629 c          607 g          389 t
ORIGIN      
        1 acgaagcgcc cccggaagca gcccggactc acagaggatc tggaatccat tgcagatacc
       61 gatgacgggg cccccgtcgg ccgcgaaagt gcgcacagca tccatgatcg gagagaaggc
      121 ggcgatcgct ccggttctca ggtaatcgcc atacgagaac ccgcccggca gaacgacggc
      181 ctcgaagtcc gacaggtcgg tctgacggtg gaacacgtac tccggctcgg cccccaacaa
      241 cctcagcgca tgcacggcgt cctgctcaca attcgatcct gggaatacga ccaccgcgac
      301 gcgtctgctc aaagagcctc ctcgagcacc gttgttctga tgtggaactg ctcgatgacg
      361 ggattggcca acatacgcgc acacgcggcc tctatccgcg ccactgccgc ggcttcggaa
      421 tccgcctcga tgatgaaggt gaacaacttg ccggcgcgaa gttcgctgat ccccgtgaag
      481 ccgaggcgcg ggagggcctg ctcgatggtt agtccctggg ggtccgagat cccttccttc
      541 ggggttacct cgactgccac tgagaatctc aaaccacacc gcctgcatcc acaggctcgc
      601 cggttagtcg ttcgagggcc tcgacatact tggcttgagt cccttcgatg acgtcagcag
      661 gaaggtccgg gggagggggc gagtgatccc atccctgcga gtccaaccaa tcgcgcagga
      721 actgcttgtc gaatgagggc tgcgagcggc ccggttccca actatccgct ggccagaacc
      781 tcgaagagtc gggcgtcaac acttcatctg caagtacgac cttgccgccg aggatcccga
      841 actcgaactt cgtatcagcc aggatgatcc ccctggtggc ggcgtgatcg gatgcccgtt
      901 cgtagatgtc tagggcatag ccgcgagcta tcttcagcac atcgcccccg atgatgtcgg
      961 ccgccatgtc ctctgagatg ttctcgtcgt ggccgctcgt ggccttcgtc gcaggcgtga
     1021 agatgggttc gggcaatcgg tcggactcgc gcagacccgc tggcagcttg acctcgcaga
     1081 ccgtcccggt ggtctggtac tccttccaac ccgagccagc gaggtagccg cggatcacga
     1141 actcgaccgg aagcatctcg agtctgtcga cgacgagcga gcgctctctg atgtcatctg
     1201 aaaccgcatc cggaagctca tcgttcgcag cgagatgatt aggacagatc tccgccatga
     1261 ggtcgaacca gaacctgctg agctgagtga ggatccgccc cttctgtggg atgccctgag
     1321 acatcaccac gtcgtaagcg gatatccggt cggtggcgac gaagaggagt cttccatcag
     1381 caagatcgaa gacctctcgc acctttccgg aagccgatcg gatgaaggga agatcgacgt
     1441 tgagcagggc ttcggtcatt ttcatctctc cagatcttcg agtcggtcga tgatgctctg
     1501 cgagtgttca agatatcgcg attcttggaa gcaaaggtcg aggtccgagg ccgacatcac
     1561 cgcggcaacg ttgtcatccg aggccagcag gtctcgcagc tgtgccttcg aatcccaggc
     1621 cctcatggca tcctcctgga cgatccggta ggcctcgtcc cgagtaaggc ccttgtccac
     1681 gagaccgaga aggaccgact gcgagaagac gagcccgtag gaaccctcga tgttctcgcg
     1741 catcctctcg gggtagacat tcagtccgag cagaaggtcg gtcgactcgg cgatcatgaa
     1801 gtcgagaagg atgcatgtgt ctgggagcga tacccgctcg accgaggagt gggagatgtc
     1861 gcgctcatgc caaagtgcga tgttctcgat cgccggaaga accgcggcac gaacgagtct
     1921 ggcgagcccg gtgagtcttt cggtcctgat ggggttgcgc ttgtgtggca tcgccgatga
     1981 ccccttctgc tctccttcgg cgaatggctc ctccacctct cgcacctcc
//
LOCUS       DATDOZ010000121         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3774847, whole genome shotgun sequence.
ACCESSION   DATDOZ010000121 DATDOZ010000000
VERSION     DATDOZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774847"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VI541_02305"
     CDS             <1..451
                     /locus_tag="VI541_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HLF69770.1"
                     /translation="TSSGFESWLAMRGLATLGLRVERHCSNALAVATFLEAQPSVSRV
                     LYPGLASHPQHERASKLLTGYGGMVAVDLGSQEGAWRFMDGTQVARVGSSFGGVRSEL
                     THPASTSHRQFSTDDRLTAGITDGLVRIAVGLEDIDDLLADFDGALR"
     gene            448..1548
                     /locus_tag="VI541_02310"
     CDS             448..1548
                     /locus_tag="VI541_02310"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HLF69771.1"
                     /translation="MRRTRVAIIFGGRSGEHEVSCVSARHVAAALDPEKFEVIPIAIS
                     RYGEWLLPEVSRKVLEGGHLEVPAVAFEAEGEPVTLDSSSHQIVPSDRSSAVAPISDI
                     DVVFPVLHGPNGEDGTVQGLLELMDVAYVGSGVLGSALGMDKEKMKIVLRAAGLPGVE
                     FVTVRESEWRSDMKALLHRAGALGFPLFTKPANLGSSVGISKCKDALSLVAGIEEALR
                     HDRKVLIEEGISGRELEVGVLGNDEPIASVVGEIIPAREFYDYEAKYSDPTSQTRIPA
                     ELPSEVSDQVRKYSIAAFRAIDASGLARVDFFYEEAGRGVLVNEINTIPGFTTISMYP
                     KLWEASGIAYPDLVSRLLDLAQEKHARKPRPV"
     gene            complement(1570..1806)
                     /locus_tag="VI541_02315"
     CDS             complement(1570..1806)
                     /locus_tag="VI541_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLF69772.1"
                     /translation="MAVQAYILIQTEVGKAAQVAKEVAAISGVQAAEDVTGPYDVIVQ
                     AEAATVDDLGKLVVAKIQAVEGITRTLTCPVVHL"
     gene            2077..>2449
                     /locus_tag="VI541_02320"
     CDS             2077..>2449
                     /locus_tag="VI541_02320"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HLF69773.1"
                     /translation="MTADGAGAKALAELGITTSGSTLIDPEVGSLIDAAVANGEGRVA
                     ANGALVARTYERTGRSPKDKFVVRDPQTSERVWWDNNEAIAPASFQSLWELAIRHLCD
                     RDVYVVDATAGAEPQARIGVTL"
BASE COUNT          477 a          744 c          774 g          454 t
ORIGIN      
        1 cacgtcctca ggattcgaga gttggcttgc catgcgaggg cttgcgacac tcggtctgcg
       61 ggttgaacgc cactgctcga atgctctggc agtcgcgacc ttcctcgaag ctcagccctc
      121 ggtgagcagg gtgctctacc caggactcgc gagccatccg cagcatgagc gcgcctctaa
      181 gcttctcacc ggttatgggg gcatggtcgc ggtagacctt ggatctcagg agggggcctg
      241 gcgattcatg gatggcacgc aggttgcgag agtcggatcc tcgttcggag gtgttcgcag
      301 cgagctcacg catcccgcgt cgacgtctca tcgacagttc tcgaccgacg accggctgac
      361 tgctggcatc accgatggcc tcgtgaggat cgcggtcggg ctcgaggaca tcgacgacct
      421 tctcgcagac ttcgacgggg cgcttcgttg agaaggacac gagttgccat catcttcggg
      481 ggccgctccg gagagcatga ggtctcatgc gtctctgcgc gccacgtcgc cgccgctctc
      541 gatccggaga agttcgaggt gatccccatc gcgatctcgc gttatgggga gtggctcctc
      601 cccgaggtgt ctagaaaggt cctcgaaggt ggccatctcg aggtccctgc cgttgccttc
      661 gaagccgagg gagaaccggt gactttggac tcctccagcc atcagatcgt tccatcggac
      721 aggtcgagtg cggtcgcccc catctccgac atcgatgtcg tctttccggt gctccatgga
      781 cccaacggcg aggacggcac agtccaaggg ctcctcgaac tcatggatgt ggcctacgtc
      841 ggttccgggg tcctggggtc ggccctcggt atggacaaag agaagatgaa gatcgtcctg
      901 cgggctgccg gtctgccggg ggtcgagttc gtcactgtgc gagagagcga gtggaggagc
      961 gacatgaagg ccctacttca ccgcgcaggc gcactcgggt tcccactatt cacgaaaccg
     1021 gccaacctcg gttcgtccgt tggtatcagc aagtgcaagg acgctctgtc cctggtcgcc
     1081 gggatcgagg aagcccttcg tcatgatcgg aaagtcctaa tagaggaagg gatatcgggc
     1141 cgcgaactcg aggtcggggt gctgggcaac gatgagccca tcgccagtgt tgtgggcgag
     1201 atcatccccg cccgtgagtt ctacgactac gaggcgaagt acagcgaccc gacgtcgcag
     1261 acacgtatcc cggcggaact gcccagcgaa gtttcagatc aggtacgcaa gtactccatc
     1321 gccgcgttca gagcgataga cgcctccggg ctcgcgcgtg tcgacttctt ctatgaggaa
     1381 gcgggcaggg gtgtgctagt caacgagatc aatacgatcc cggggttcac gacgatctcg
     1441 atgtacccga aactctggga ggcgagtggg atcgcctacc ctgacctggt atctcgtctg
     1501 ctcgacctcg cgcaggagaa gcacgcgcga aagccgcgac cggtctagtc cctcagcctg
     1561 cccgctcgat cagagatgaa cgactggaca tgtgagcgtg cgagtgatgc cctcgaccgc
     1621 ctggatcttg gcaaccacca gcttgcccag gtcatccacc gtggcagctt ccgcctgaac
     1681 gattacgtcg tatggaccgg taacgtcctc tgcggcttgg acgcccgaga tagcggcgac
     1741 ctccttggca acctgtgcgg ccttgccgac ctcggtctgg atgagaatgt aggcttgaac
     1801 cgccatggtt cctccttggt aagtagggtg cgtatcctac tcagacgcgt tggttcctgc
     1861 tagcgaaatc tataatccgg gggccatgcc tgcatccaag cccccaccga agacgaaggc
     1921 cccaagcttc aaagaccgat acgatcgcat ccgaaagccg gtcccccctc cgtcgcgagt
     1981 ggagcccgat agacgcaaga agatacgcga gagcatcgct cggaaggagt ccgagggaga
     2041 tcccgcagca gactcgaacg aaaggaagcc gaacagatga cggccgacgg ggcgggagct
     2101 aaggctctgg ccgaactagg gatcacgacg tcaggcagca ccttgataga ccccgaggtc
     2161 gggtcgctca tcgacgcagc tgtcgcaaac ggggagggca gggtcgccgc caatggggcc
     2221 ctcgtcgccc gaacctacga gagaaccggc cgctcaccaa aggacaaatt cgtcgtccgg
     2281 gatccccaga cctcagagcg ggtgtggtgg gacaacaacg aggcgatcgc ccccgcgtcc
     2341 ttccaatctc tctgggagct ggcgatccgg catctgtgcg atagggatgt ttatgtggtc
     2401 gatgcaaccg ccggggccga gccgcaggcg cgtatcggtg taaccctca
//
LOCUS       DATDOZ010000122         3526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2207393, whole genome shotgun sequence.
ACCESSION   DATDOZ010000122 DATDOZ010000000
VERSION     DATDOZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207393"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VI541_02325"
     CDS             complement(<1..374)
                     /locus_tag="VI541_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLF69774.1"
                     /translation="MAVSQDQAVILAGGQATRMRPYTDDRPKAMVELGGRPIIEHQIE
                     WLRQHGVSTLVVSCGYRADLLQSHLGDGSSFGVAITYAVEETPLGRGGGLKYAAGHLP
                     YPDLPWFALNGDVIARFDLREMA"
     gene            422..1759
                     /gene="ccrA"
                     /locus_tag="VI541_02330"
     CDS             422..1759
                     /gene="ccrA"
                     /locus_tag="VI541_02330"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HLF69775.1"
                     /translation="MSIDQIRSAIEAGDFAGAASVPLPESIRAAFVRKDEQEIFDGLD
                     HVDKDPRKSLHVGDVPLPPVGPNEALVAIMASAINYNTVWTSIFEPIPTFRFLERFGK
                     ESELGRRHDLDHHIVGSDGSGVVLKVGSGVTRWKPGDLVTVHCNYVDMEAPEGHDDSM
                     LDPSQKIWGFETNFGGLAEIAMVKANQLMSKPRHLTWEEAACNGLVNSTAYRMLVSQN
                     GTNMKQGDVVLIWGATGGLGGYAIQYCLNGGAIPVAVVSSPEKVALLASLGVERVIDR
                     SAEGYEFWMDGQQQPKEWMRFGKKIRELTGGEDPDIVFEHPGRQTFGASVFVTKRGGK
                     IVTCASTSGFTHEYDNRYLWMHLKSIIGSHFANYREATEANRLLDLGMIQPILSRVFP
                     LAEVAQAAYDVHTNAHVGKLGILCLAGAEGQGVQDDSKREALGTKVTAWKSLS"
     gene            1814..>3526
                     /locus_tag="VI541_02335"
     CDS             1814..>3526
                     /locus_tag="VI541_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLF69776.1"
                     /translation="MDRRLKVRWASWLAGLVLASMIVQPAQAAPVGFPQFVNGGNSSI
                     WHVRNDGGTSGDDISSVRRCDSTKPGFTVYDGSLNATSTVERAFDTGATLWVKGSGPT
                     DPFVQFNATATTGTVDLVGRTITTGIQTLSGLTTRMQYYVATNTATLRTLATFENPST
                     STVTVTVSFQSNSGAEDQAQIPTTMAGVVTGTSSGDSNLTTADRWALQGHTVETTTAM
                     VTHVLYGPNSPSVTPSAASTLVSFACPAPVPPLPGSGSKDGIRADYILGVPGPGVRSL
                     MWFNQLHITAAEASTAAVSTFDNNGSLGADILSGLTPTDLFRVANWKFAFTRFIATSS
                     GAGERSLVSVWALDPVTGLQSQFIPPFEAYPGFFGGVRVAMGDLDGDGKPELITAAGP
                     GGGPHVRVFDFFPASATVTPRSGGGFFAFDSSFRGGVNVAVGDIDGDGTGEIITGQGP
                     GGTSRLRAFHIDPTTGTRSTYFDEDAYEEFTGGMTVATGELHGNNGGVEIVTGAMGGG
                     GPRVRIFQFDFVGALVQLPSFFAYDKNFSGGVDVAVGDVDGDGTKDLITGAGPGGGPH
                     VKVFK"
BASE COUNT          695 a         1063 c         1084 g          684 t
ORIGIN      
        1 gccatctctc gcaggtcgaa gcgcgctatg acatcgccgt tcagcgcgaa ccagggcaag
       61 tcggggtagg ggagatggcc agccgcgtac ttgaggcccc cgcccctgcc caacggggtc
      121 tcctcaaccg cgtatgtgat cgcaacgccg aacgacgagc cgtcgcccag atgcgactgg
      181 agaaggtcgg ctctgtagcc gcaggagact acgagagtgc tcactccatg ctgccggagc
      241 cactcgatct ggtgctcgat gatgggacgt cccccgagct cgaccatggc cttcgggcgg
      301 tcgtccgtgt acggtcgcat gcgtgtcgcc tgtccgcctg caagaatgac cgcttgatct
      361 tgtgatacag ccatggagca actctagttg agttcgctgc tgtcgcctgg gaggccgaaa
      421 gatgtcgatc gatcagatcc gtagtgcaat cgaagccggt gacttcgccg gggcggcatc
      481 ggtccccctc cccgagagca tccgcgcggc cttcgttcgc aaggacgagc aggagatctt
      541 cgatggtctc gatcatgtcg acaaggatcc tcgcaagtcg cttcacgttg gcgatgtgcc
      601 gcttccgccg gtcggcccca acgaggccct cgtggcgatc atggcgagtg ccatcaacta
      661 caacacggtc tggacctcga tcttcgagcc gatcccaact ttcagattcc tagagaggtt
      721 cggcaaggag tccgagttag ggcgacgcca cgacttggat catcacatcg tcggttccga
      781 tggctcgggg gtcgttctca aggtcggttc cggtgttact agatggaagc ccggcgacct
      841 agtgaccgtc cactgcaact acgtcgacat ggaagccccc gagggccacg acgactcgat
      901 gctcgacccg agccaaaaga tatggggatt cgagacgaac ttcggcggcc tcgctgagat
      961 cgcgatggtc aaagccaatc agttgatgtc caagcccagg catctgacct gggaagaggc
     1021 cgcatgcaac ggactcgtca actcgacggc ataccgcatg ctcgtcagcc agaacgggac
     1081 caacatgaaa cagggcgatg tcgtgctcat ctggggcgcg accgggggcc ttgggggcta
     1141 tgccatccaa tactgcctca acggaggagc catacctgtc gctgtcgtct cgtcccccga
     1201 aaaggttgca ctccttgcat cactaggtgt ggaacgcgtc atcgaccgtt ctgcggaggg
     1261 gtacgagttc tggatggacg gccagcagca gcccaaggag tggatgcggt tcggcaagaa
     1321 gatacgcgag ctgactgggg gcgaggaccc cgacatcgtc ttcgagcatc cagggcgtca
     1381 gaccttcgga gcaagcgtct tcgtgacgaa gcgagggggc aagatagtca cctgcgcgtc
     1441 gacatcgggc ttcactcatg agtacgacaa ccggtaccta tggatgcacc tcaagtcgat
     1501 catcggatcg cacttcgcca actatcgcga ggccaccgag gccaaccgcc tcctcgatct
     1561 tgggatgatt cagcccatct tgtccagagt cttccccctt gcagaggtcg cacaggccgc
     1621 atacgacgtg cacaccaacg ctcatgttgg caaactaggg atactctgcc tcgcgggggc
     1681 cgagggccag ggtgtccaag atgactcgaa gcgagaggct ctaggcacta aggttacggc
     1741 ttggaagtcg ttgtcatgat ctgtgctcat ctatcgcacc agcacgtcga cgaaggacgg
     1801 ataggggttg ttcatggata ggagattgaa ggtcagatgg gcctcgtggc ttgcgggcct
     1861 ggttttggcg tcgatgatcg tgcaacccgc gcaagccgcc cctgtaggtt tccctcaatt
     1921 cgtcaacggt ggcaacagtt cgatctggca tgtccgcaac gacgggggga catcgggcga
     1981 cgacatatca tcggtgcgac gctgcgattc gacgaagccc gggttcacgg tttatgacgg
     2041 ctcgttgaac gcgacaagca ctgtcgagcg agccttcgat acgggcgcga cgctctgggt
     2101 gaagggttcc ggtcctaccg acccattcgt tcagttcaat gcgacagcaa ctacggggac
     2161 cgttgacctt gttggtcgca cgatcaccac gggcatccag acactctcgg gattgacgac
     2221 tcgcatgcag tactacgtcg cgaccaatac tgcgaccctt cgcaccctcg ctaccttcga
     2281 gaacccatcg acatcgaccg tgaccgtgac cgtgagtttc cagtcgaact cgggagccga
     2341 agaccaggcc cagatcccaa ccaccatggc gggagtcgtt acggggacga gcagcggtga
     2401 tagcaacctc accactgccg ataggtgggc gctccagggt cacaccgtcg agacgacgac
     2461 agccatggtc acgcatgttc tgtatggtcc caactcgcct tcggttacgc cctcagcagc
     2521 ctcgacgctc gtttcattcg catgcccagc accagtgccc ccactcccag gaagcggatc
     2581 taaagatggg atccgggccg actacatcct tggcgtccca ggccccgggg tccggtccct
     2641 gatgtggttc aaccagcttc acatcacagc ggccgaggcc tcaacagccg cggtgagtac
     2701 cttcgacaac aacggtagcc tcggtgccga catcctctcg ggtttgaccc caacggacct
     2761 cttcagggtc gccaactgga agttcgcgtt caccaggttc atcgcgacga gttcaggtgc
     2821 gggcgagagg tccttagtct cagtctgggc tctcgacccg gtaactgggc ttcaatcaca
     2881 gttcataccg ccattcgagg cctaccctgg attcttcggc ggcgtacgcg ttgcgatggg
     2941 cgacctcgat ggcgacggca agccggaact catcaccgcg gccggtcccg gtggggggcc
     3001 ccatgtgagg gtcttcgact tcttcccagc gagcgctacc gtcacaccgc gttcgggagg
     3061 gggcttcttt gcttttgact cgagtttccg tggcggagtg aatgtcgcgg tgggcgacat
     3121 cgacggtgat ggaacgggtg agatcatcac cggtcagggt cccggcggga cgtcgcgtct
     3181 tcgtgcattt cacatcgacc caacgactgg aacccgctct acctacttcg atgaagacgc
     3241 ctatgaggag ttcaccggag gcatgactgt ggcaacggga gaactccacg gaaacaacgg
     3301 cggcgttgag atcgtgaccg gtgccatggg gggcgggggc ccacgagtca ggatctttca
     3361 attcgacttt gtgggagccc tggtccagtt gccctctttc ttcgcttacg acaagaattt
     3421 cagcggcggc gttgacgtcg cggtgggcga tgtcgatggc gacgggacca aggacctcat
     3481 cacgggcgcg gggccgggcg gggggcccca tgtgaaggtt ttcaag
//
LOCUS       DATDOZ010000123         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2726645, whole genome shotgun sequence.
ACCESSION   DATDOZ010000123 DATDOZ010000000
VERSION     DATDOZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726645"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VI541_02340"
     CDS             complement(<1..198)
                     /locus_tag="VI541_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69777.1"
                     /translation="MEHVLIRKLDHLTGSATSPRLGFAVEARDRPGPAHKNGAFEGDV
                     VWVQLHGGLFVARAKVKICWIG"
     gene            complement(201..1298)
                     /locus_tag="VI541_02345"
     CDS             complement(201..1298)
                     /locus_tag="VI541_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLF69778.1"
                     /translation="MTRLAISHSAINLAQGFPDFACPPELKSAAIEAIEADLNQYAIT
                     WGAEPFRRAIAAKTQRFYPGWEVDPETQITVTCGATEGMIAAMLAVVNPGDEVIIFEP
                     FYENYGPDAVLSDAVPRFVTLHEPDWSIDEGELRRAFDTKTKGIVVNTPHNPTGKVFS
                     SDELQLIADLCLEHDVIAFTDDIYEHIIYAGKHLPLARFDGMAERTIAINAMSKTYSV
                     TGWRVGWVIADEKLTSGIRKVHDFLTVGAPAPLQAAGAVALAMPDSFYEKLAVEYQER
                     RDLLVPALEGAGLKPFTPEGSYYVMCDIRGVTDLDDVAFARKMVAEPGIATVPGSSFY
                     SRPELGADKVRFAFPKKTETLEAAAERLKRI"
     gene            1423..>2278
                     /locus_tag="VI541_02350"
     CDS             1423..>2278
                     /locus_tag="VI541_02350"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HLF69779.1"
                     /translation="MTKRILRILGVALAYYLAARLGLRLSLVGHNVTPIWPPTGIAVA
                     AFLIFGYRVWPGVALAAFVVNAPITPSLGSTLLITAGNTAAPLASIFIMRRFGGAANL
                     RRAKEVLVFVFGGALLAMTISAGVGSSALRLAGVISEAQHLSTFLVWWTGDSMGVLVF
                     APALLLLRESHWPKGAKTWRAVEGLLLCLLFVLTTDAVLSSTGHYLFLIFPLIIWAAV
                     RFQRTGAVVAVAFVSLMAARAAAMLVGPFEGLVLSRLMLTLNALNGSLALTGYLLAAV
                     TIERLQAQE"
BASE COUNT          382 a          698 c          707 g          491 t
ORIGIN      
        1 gccgatccaa cagatcttca ccttcgctcg cgccacgaag agcccaccat gcagctgcac
       61 ccagacgacg tcaccctcga aagcgccgtt cttgtgagcg gggccgggcc tatcgcgtgc
      121 ctcgacggcg aagccgagcc gggggctggt cgccgatccg gtgaggtgat cgagttttcg
      181 tatcagcacg tgctccacgg ttagatcctc ttcagtcgtt cggccgccgc ttcgagtgtc
      241 tcggtcttct tggggaaggc gaaccggacc ttgtcggcac cgagctcagg gcgtgagtag
      301 aacgaggatc ccgggaccgt tgctatgccg ggctccgcga ccatcttgcg agcgaacgca
      361 acatcgtcaa ggtcggtgac gccccggatg tcgcacatga cgtagtagga gccctccggt
      421 gtgaacggct tgagcccggc cccctcgagc gccggaacaa gaaggtcgcg tcgctcttga
      481 tactcgacgg caagcttttc gtagaaagag tccggcatcg ccagtgctac cgccccagcc
      541 gcttggaggg gtgcgggggc cccgaccgtc agaaagtcat ggacctttct gatccccgat
      601 gtgagcttct cgtcggcgat cacccatccg accctccacc ccgtcaccga gtaggtcttc
      661 gacatggcgt tgatcgctat cgtgcgctca gccatcccat cgaagcgcgc tagcggcaga
      721 tgtttgcccg cgtagatgat gtgctcgtag atgtcatccg tgaatgcgat cacatcgtgc
      781 tcgaggcaga ggtcggcgat cagctgaagc tcatcgctgg agaaaacctt gcctgtgggg
      841 ttgtgtggcg tgttgaccac gatccccttg gtcttcgtat cgaaggcccg ccgaagttcg
      901 ccctcgtcga tgctccagtc aggctcatgc agagtgacga atctcgggac agcgtcggag
      961 agcactgcgt ccgggccgta gttctcgtag aaaggctcga agatgatgac ctcgtctccg
     1021 gggttcacga ccgccagcat cgccgcgatc atcccctcgg tcgcgccgca ggtgacggtg
     1081 atctgggtct cggggtccac ttcccatcct ggatagaagc gctgcgtctt ggctgcgatg
     1141 gcccgacgga agggttcggc cccccaggtg atcgcatact ggttgaggtc ggcctcgatg
     1201 gcctcgatgg ccgcggactt gagctcaggg gggcaggcga agtctggaaa accctgggcg
     1261 aggttgatcg cactgtggct gatggcgagt cgcgtcatct cgcggatgac cgattccgtg
     1321 aaactgcctg ctctgtcgga gatgtttacc accgtttaga acctcctata tgactatatc
     1381 gggccattcc agggagaggc tctcgaatcc gatacttagt tcatgactaa gcgaatcctg
     1441 aggatcttag gagtcgccct tgcctactac ctggccgccc gccttggcct gcggctctcg
     1501 ttggtaggtc acaacgtgac gcccatatgg cccccaacgg gtatcgctgt cgcggcgttc
     1561 ctcatcttcg gatatcgcgt ctggccgggt gtcgccctgg cggcattcgt cgtgaatgct
     1621 cccatcaccc catctctcgg atcgacgctc ctcatcactg ccggcaatac ggcggcaccc
     1681 ctcgcaagca tcttcatcat gcgcaggttc gggggcgcgg ccaacctaag gcgtgcgaag
     1741 gaggtactgg tcttcgtctt cgggggcgct ctgctggcga tgacgatcag tgcaggcgtc
     1801 ggatcatcgg cccttcggct ggctggtgtg atcagcgagg cccaacacct atctaccttc
     1861 ctggtctggt ggacggggga ttcgatgggg gtcctggtct tcgctccggc cctactgtta
     1921 ctccgggaaa gccactggcc caagggggcc aagacatggc gggcggttga gggtctgctg
     1981 ctctgcttac tattcgtgtt gactacggac gcggtcctgt cttcgacggg tcactacctg
     2041 tttctcatct tcccgctcat catctgggct gcagtgaggt tccagcgaac gggggccgtc
     2101 gtggccgtcg cattcgtctc gctcatggcg gcaagggccg cggccatgct agtcggccca
     2161 tttgaaggtc tcgttctgtc gcgactcatg ctgactctga acgcgttaaa cgggtctcta
     2221 gcactcaccg ggtatctgct tgcggccgtg acgatagagc gcttgcaggc gcaggaat
//
LOCUS       DATDOZ010000124         6724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1929776, whole genome shotgun sequence.
ACCESSION   DATDOZ010000124 DATDOZ010000000
VERSION     DATDOZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929776"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="VI541_02355"
     CDS             complement(<1..1695)
                     /locus_tag="VI541_02355"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HLF69780.1"
                     /translation="MLARALARLRRTKVAEDSIALRVAVASAVLASMIGVIAQDIVSG
                     PVAFLVLVLTPLGYLFSWYRRSSRNIVLKVLLAAGAVMALANFLQRLGAATSVEAARA
                     PLAEIFLYVQMLHSFDLPRRRDLHFSLASSITLIAMGGSLALSGDYVFFFVPWGVAAL
                     VALHLSYASEVGLRSGARPDATSRRVGLPGIRVIAAGIVVVILATATVFMLLPRTGGS
                     KLTASFQLPSLGKLLPVGSGIVNPSFPTLFGSPDGPFGVGRGGYYGFADQLDLRIRAR
                     LSDEIVLRVRAPQAQFWRGAVFDVYSNSMWTQSNDRKRALTGLHAFIPSEPANAINLR
                     GSSEMLQTFYVEQPQPNLVFAAYRPEEVWFPVGGVRVNDRMSLQAPVVLPPGTVYSVL
                     SRVPTPSGGDLAVTTEGTPPALLSRYTQLPSELPARVHHLAAELAGPHPTIIAKADAI
                     QRWLRSNTRYKIDIPPQPRGTDAVDHFLFEDRRGYCEQMATAMTVMLRSQGVPARLAT
                     GYDSGDRDLLSGYFEVKQSDAHSWVEVYFPGTGWIEFDPTHAVPLASRGAEIPAAAA"
     gene            complement(1668..2879)
                     /locus_tag="VI541_02360"
     CDS             complement(1668..2879)
                     /locus_tag="VI541_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLF69781.1"
                     /translation="MKLRPKRRMLTLSLGGALLLLVASNAQAGWLYVVSAAMFGIAIA
                     GALMPALHLRGLRFERELPQTVRVGDALRVTLRVAAGRGWSRGPLFGRDLLTDSEVVI
                     DRVSSRSHGLIRYDFVPQRRGHFSGGQIEMTTGAPFGVVEATRRWEVPSELLVHPRWV
                     QVGNLPLLESASVPDEDMHDRSRRGEGLEVFGLRDYRRGDSVRHIHWRSSARGGGLLV
                     REYEQPPSSRLVILIDSHEDIGTGADSTFEVSVSIAASIAMYGLDVGHPIEMVCGGSP
                     EGRLVEPGKLAALDWLAKLEGGVGRPLSDLVMTADLHTLRRSTAVLIFPLTVAAERDV
                     GSAVSALQEAGARVLAMAVSARAAGAAHASLGAHDAAELLTRLRQSRAIVYGIDPSRE
                     LTDCLREPLHV"
     gene            complement(2876..3820)
                     /locus_tag="VI541_02365"
     CDS             complement(2876..3820)
                     /locus_tag="VI541_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLF69782.1"
                     /translation="MDRQAAAEALKRIASNVEVVILGKRSVIETCVLALACEGHVLIE
                     DAPGVGKTMLAKALARSIDGVFKRVQATPDLLPSDITGVAVFDQADREFKFMPGPIFA
                     NVVLVDEVNRTTPRTQSALLEAMEERQVTAEGATRPVPTPFFVIATQNPLEYHGTYPL
                     PEGQLDRFIMATSMGYLAQADERSVVRSQLLDHPIESLGPVVTLHEIEEIAACVRETK
                     VTDVVLDYIIGIVAATRTHPDVDLGAGPRGSLGLTRVAQARALLVGRDFVAPDDVKAV
                     AAMVLGHRLVLKPQLRRGPLAGRALIRDLLTKLPVSVG"
     gene            complement(3865..5076)
                     /locus_tag="VI541_02370"
     CDS             complement(3865..5076)
                     /locus_tag="VI541_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLF69783.1"
                     /translation="MSSSLRHISLRLGLATVMVALAVAPAQAALTDRQNQIESQKRAL
                     RTKIADADERAGIITAQIRQSDARRAALEGQIKDLTGQLATATARLVQAEADLGVARQ
                     DLIRLEGDLSITLVRLEALKERADERQRLVYQQGGTGSYISLLLGADNMRDFIGRLHY
                     VRTVMSDDKSRIDGLRKLGNQLEASRTEVVTRKDEITKQKISIEAERSNIKKLQDQLK
                     TSRAQVDAEIATRKQLLSKVQSEKASYLRQVAQLEAESRSISALLRSRQRGQIFKAGS
                     GVRLAWPTTGRISSYYGWRTHPIFGDRRMHTGIDISAPSGQSVQSAEAGSIVYVGTKG
                     GYGTVVIIDHGSALATLYAHLSATSVSSGQTVTRGQRVGSVGCSGYCTGPHLHFETRI
                     NGEPKDPMQFF"
     gene            complement(5151..6032)
                     /gene="ftsX"
                     /locus_tag="VI541_02375"
     CDS             complement(5151..6032)
                     /gene="ftsX"
                     /locus_tag="VI541_02375"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HLF69784.1"
                     /translation="MALRVGYFLSETFINLRRNLLMTLAAITTVSVSLFLLGGVMVIG
                     EIVNRAVSRWEGKVELSVFLREEITPDQTKRLSEMISKMPETQHVFYETKEEAFKEYK
                     RMFADQPALTENVTSDALPASFRIKLRDPNAAEAVQRVLGGQPGVDQVQFGGDAVKKL
                     LRVTRLFRTVSLATTAILLMAATMLIANTIRLAIYARRKEVGIMKLVGATNWFIRVPF
                     IFEGIAESLLGAALATGVIYAGKTLILDQLQREIVFLPVTVGPLVIVRIFISLLAVGI
                     VIGAIGSSLALRRFLEV"
     gene            complement(6033..6722)
                     /gene="ftsE"
                     /locus_tag="VI541_02380"
     CDS             complement(6033..6722)
                     /gene="ftsE"
                     /locus_tag="VI541_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HLF69785.1"
                     /translation="MIRLESVTKLYKDSVVALNCVSADIQKDEFVFLVGPSGSGKSTF
                     MRLLLKEEEPSEGRIYVAGKDIAQLPNWRVPYLRRNIGCVFQDFRLLSNKTVGENVAF
                     ALEVIGRPRHVIGQRVPEILDLVGLSDKINNRPDELSGGEQQRVSIARAIVNRPPILL
                     ADEPTGNLDPATSTDIMALLDRINQSGTTVLMATHDHAMVDQARKRVIELDSGKIVRD
                     QVRGVYGSGGF"
BASE COUNT         1356 a         2091 c         2034 g         1243 t
ORIGIN      
        1 ggcagccgcg gccgggatct ccgccccccg gctcgcgagc ggcactgcgt gagtgggatc
       61 gaactcgatc catccggttc cggggaagta gacctcgacc catgagtgcg cgtcgctctg
      121 cttgacttcg aagtaacccg acaacaggtc cctgtcgcca ctgtcgtaac cagtggctag
      181 tcgggcagga acaccctggc tgcgaagcat caccgtcatc gctgtcgcca tctgctcaca
      241 gtatccacgg cgatcctcga agaggaagtg atccacggcg tcggtgccac ggggctgcgg
      301 agggatatcg atcttgtatc gcgtattgct gcgcagccat cgctggatcg catccgcctt
      361 agcgatgatc gtcggatgcg ggcccgcgag ttcggcagcc agatgatgaa ctcgcgccgg
      421 cagttcggat ggcagctgcg tatagcgcga gagcagggcc ggtggggtcc cttcggtggt
      481 aacggccagg tcaccacccg acggcgtcgg cacccgcgac aagacgctat agacggtgcc
      541 aggaggcaag accacggggg cctgaagact catcctgtcg ttaacacgaa cacccccgac
      601 tgggaaccac acctcctcgg gacgataggc agcgaagacg aggttcggct gcggctgttc
      661 aacatagaag gtctgcagca tctcgctgct gcccctgagg ttgatcgcat tggcgggctc
      721 cgaggggatg aacgcatgca ggccagtcaa tgcgcgcttg cggtcgttgc tctgcgtcca
      781 catgctgttg gagtagacat cgaagaccgc accgcgccag aactgtgcct gcggtgcgcg
      841 aacccgaagg acgatctcgt ctgagagcct cgcgcggatc cgaaggtcga gctggtccgc
      901 gaagccgtag tacccaccac gtccgacccc gaagggccca tccggcgagc cgaacaacgt
      961 ggggaatgat gggttcacga tccccgaacc gaccgggagc agcttgccta gactcggcag
     1021 ctggaacgac gcggtgagct tggatccccc tgttcgcggc agcagcatga aaaccgtcgc
     1081 ggtcgcgagg atcaccacga cgatccccgc ggcgatgaca cggataccag ggagacccac
     1141 acgccgcgag gtcgcatcgg gacgggcccc cgatcgcagc cccacctctg atgcatagct
     1201 gagatgcagg gcaacaaggg ctgcgacccc ccacgggacg aagaaaaaga catagtcgcc
     1261 actgagggcg agagatccac ccatggcgat cagcgtgatc gaggatgcga gcgagaagtg
     1321 aaggtcacgt cttcgcggga gatcgaaaga gtgaagcatc tgcacataga ggaagatctc
     1381 ggcaagcggg gcacgcgctg cttcaaccga ggttgcggca cccaaacgct ggaggaagtt
     1441 cgccagagcc atcaccgcac ccgcagccag caacaccttc aagacgatgt ttcgcgacga
     1501 gcgtctgtac caagagaaga ggtagccgag cggggtcagc acaaggacaa ggaacgccac
     1561 cggcccgctg acgatgtcct gcgcgatgac accgatcatg ctcgccagca cggccgatgc
     1621 aacagccacg cgaagagcta tcgaatcttc ggcgaccttg gttcttctca gacgtgcaag
     1681 ggctcgcgca agcaatcggt caactccctc gatggatcga tcccgtagac gatggccctg
     1741 ctctgtctca accgagtgag aagttcagcc gcatcatggg caccaaggct agcgtgggcg
     1801 gcaccggcag ccctggccga cacggccatc gcaagaaccc gcgcacctgc ctcctgcagc
     1861 gccgagacgg ccgaccccac atcgcgttca gcggcgactg tgagcgggaa gatcaggacc
     1921 gctgtcgaac gcctgagcgt atgaagatcc gcagtcatca cgaggtccga gagcggcctc
     1981 ccgactccgc cctcgagctt cgcaagccag tcgagagccg ccagcttgcc tggctcgacg
     2041 agtcttcctt cgggagaccc accacagacc atctctatgg ggtggccaac atcgagccca
     2101 tacatcgcga tcgatgcagc gatcgagacg ctcacttcga aggtcgagtc ggcgccggtg
     2161 ccgatgtctt catgtgaatc gatgaggatg acaagacgcg acgagggtgg ctgctcatat
     2221 tcgcgaacga gcaggccgcc tcccctggca gaggatctcc agtggatatg tcgaacgctg
     2281 tcaccgcggc ggtagtcgcg cagaccgaag acctcaagac cctcacccct tctggatcga
     2341 tcgtgcatgt cttcatcggg gacgctcgcc gactccagaa gcggaaggtt acccacctgc
     2401 acccagcgcg gatgcactag caactccgag ggcacctccc aacgtcgcgt agcctcgacg
     2461 acaccgaacg gagcacctgt cgtcatctcg atctgccccc ccgagaaatg accccgccgt
     2521 tgggggacga agtcgtatct gatcagcccg tgcgagcgcg acgagactcg gtcgataacg
     2581 acctcggagt cggtcagaag atctcgcccg aacaggggtc ctctgctcca gccacgacca
     2641 gcagcgacgc gcagggtcac tcgcaacgcg tcccccaccc ttacggtctg cggcaactct
     2701 ctttcgaatc ggagcccacg aaggtgtaag gctggcatga gcgcccctgc gatcgcgatc
     2761 ccgaacatcg ctgcactcac aacatatagc caccccgcct gtgcgtttga tgcgacgagt
     2821 agcaggagcg ctccccccag tgagagggtc aacatccggc gtttcggccg aagtttcatc
     2881 ccaccgagac cggcagcttc gtgaggagat ccctgatgag cgctcttccc gcgagggggc
     2941 cgcggcgcaa ctgaggcttg agaacgagac gatgtcccaa caccatcgcc gcgacagctt
     3001 tcacatcatc gggggccacg aagtcacgac cgaccagcag agctcgcgcc tgtgcgacgc
     3061 gcgtcaggcc cagcgagcct cgggggccgg ctccaagatc aacgtccgga tgcgttctgg
     3121 tcgcagcgac gatgccgatg atgtagtcga ggacgacatc ggtaaccttc gtttctcgaa
     3181 cgcatgcagc gatctcctcg atctcgtgga gcgtgacaac gggacccaaa ctctcgatgg
     3241 ggtggtcgag cagttgcgac ctgacgacgc tcctctcatc ggcctgcgct agatagccca
     3301 tcgatgtcgc catgatgaat cgatccagtt gtccctcggg cagtggatat gtcccgtgat
     3361 attcgagtgg attctgggtg gcgatgacga agaatggcgt tggcacggga cgcgtcgccc
     3421 cctctgccgt gacctgtctc tcctccatcg cttcgagcaa cgccgattgg gttcgaggtg
     3481 tagtccggtt gacctcatcg acaaggacga cgttggcgaa gatcggaccg ggcatgaact
     3541 tgaactcccg gtccgcttga tcgaagacgg cgacccccgt gatgtcggaa ggcagaagat
     3601 ctggtgtggc ctgaacacgt ttgaatactc cgtcgatgga gcgcgcaagg gctttggcga
     3661 gcatggtctt gccgacccct ggagcatcct cgatgagtac gtgcccttcg cacgctagag
     3721 ccagcacgca ggtctctatg accgaccgtt tgcccaggat gaccacctcg acattggagg
     3781 cgatccgttt caatgcctcc gcggcggcct gtcggtccat ctagacatta tcgccgcact
     3841 caaaggcggc tgcggaaacg gctcctagaa gaactgcatg ggatccttgg gctccccgtt
     3901 gatacgtgtc tcgaagtgaa ggtgaggacc cgtgcagtag ccagagcacc cgaccgatcc
     3961 gactcgctgt cccctcgtga cggtttgtcc cgatgaaacg gacgtcgccg acaggtgagc
     4021 gtagagagta gcgagggcag aaccatgatc gatgatgacc actgttccat acccgccctt
     4081 ggtgccgacg tagacgatgc ttcccgcctc ggcggactgc accgactggc cgctgggggc
     4141 cgagatatcg ataccggtat gcatccttcg gtcgccgaag atgggatgag ttcgccatcc
     4201 atagtaggaa gagattcggc cggtcgttgg ccacgcgaga cgaaccccag atcccgcctt
     4261 gaatatctga cctctctgcc tggaacgcag tagcgcgctg atactgcgag actccgcttc
     4321 gagttgggcg acctggcgca gatacgaggc cttctctgat tggaccttcg agagcaactg
     4381 cttgcgcgtt gcgatctcag catccacctg cgcacggctc gtcttgagct ggtcctgaag
     4441 cttcttgatg ttcgaacgtt cggcctcgat actgatcttc tgtttcgtga tctcatcttt
     4501 gcgcgtcaca acttcggtgc gactcgcttc gagttggttg ccaagcttgc gcagcccatc
     4561 gatacgcgac ttgtcgtcgc tcatgacagt gcgcacgtag tgaagtctgc cgatgaagtc
     4621 gcgcatgtta tcggccccca agagcagaga gatgtacgaa cccgtaccgc cctgctggta
     4681 gaccaaacgc tggcgttcat cggcgcgctc tttgagcgct tcgaggcgaa ccagggtgat
     4741 cgaaagatca ccctcgagcc tgatgaggtc ctggcgggcg acacccaggt cggcctcggc
     4801 ctgtacgagt cgagcggtgg cggtcgccaa ctgtccggtg aggtccttga tctgcccctc
     4861 gagcgccgct cggcgagcat cgctctggcg gatctgagcg gtgatgatcc ctgcccgttc
     4921 gtcggcgtcc gcgatctttg ttcgcagggc ccgcttctgg ctttcgatct gattctgacg
     4981 gtcggtgaga gccgcttgtg ctggcgcgac ggcgagggcg accatcacgg tcgccagccc
     5041 cagccggaga ctgatatgcc tcaatgagct actcaatcaa cacccgtcac agacattaac
     5101 aatccttccc tatcgtgaca atactccctc ggcgtccatg gggaaaccct ttagacctcg
     5161 aggaatcttc gcagggccag gctcgaccct atcgcaccga tgacgatccc gaccgccaga
     5221 agcgagatga agatccgcac gatcacgagg ggacccaccg tgaccggaag gaagacgatc
     5281 tcgcgttgga gctggtcgag gatcagagtc ttgccagcat agatgacgcc ggtggccagg
     5341 gccgccccga ggagtgactc ggcgatgcct tcgaagatga aggggacccg gatgaaccag
     5401 ttggtggccc ccaccagctt catgatgccc acctccttgc gccgtgcata gatagccagc
     5461 cggatggtgt tcgcgatgag catcgtagcc gccatgagaa ggatggccgt cgtcgcgagc
     5521 gagacggttc ggaacaacct cgttacacga agcaactttt tgaccgcgtc gccgccgaac
     5581 tgcacctgat ccacaccggg ctgccccccc agtaccctct ggactgcctc ggcggcattc
     5641 gggtcgcgca gcttgatccg aaaagaagcc gggagagcat cggatgttac gttctcggta
     5701 agcgcgggct ggtcggcgaa catccgcttg tactccttga atgcttcttc cttggtctcg
     5761 tagaagacgt gttgggtctc tggcatcttc gagatcatct cgctgaggcg tttggtctgg
     5821 tctggtgtga tctcctcgcg aaggaagacg ctgagttcga ccttgccttc ccatctgctc
     5881 actgcgcgat tcacgatctc gccgatgacc atgaccccac cgaggagaaa caggctcact
     5941 gagaccgtcg ttatcgcagc caatgtcatg agcagattgc ggcgaaggtt gatgaaggtt
     6001 tcgctcagga agtatccaac tcgaagtgcc acttagaacc cccccgatcc atagacgccg
     6061 cgcacctgat cgcgaacgat cttcccgcta tccagttcga tgacacgctt gcgagcctga
     6121 tccaccatcg cgtggtcgtg agtagccatc aggacggtgg ttccgctctg gttgatccga
     6181 tcgagcagcg ccatgatgtc ggtcgaggtg gctgggtcga ggttccccgt cggctcgtcc
     6241 gcgagcagga tcgggggcct gttgacgatg gccctcgcga tcgatacacg ttgctgctct
     6301 ccccccgata gttcgtcggg gcgattgttg atcttgtcgg agagccccac gagatcgagg
     6361 atctcgggaa cccgctggcc gatgacgtgg cggggccgcc cgatgacctc caaagcgaag
     6421 gcaacgttct cgcccactgt cttgttggaa agcagacgga agtcctgaaa gacacagccg
     6481 atgttgcggc ggaggtaggg caccctccag ttgggaagtt gcgcgatgtc tttacctgcg
     6541 acatagatac ggccctcact cggctcttcc tccttcagca gaaggcgcat gaatgttgac
     6601 ttcccggaac ccgaaggccc caccaggaag acgaactcat ccttctggat gtcggcggag
     6661 acgcagttga gcgcgacgac cgaatcctta tagagcttgg tgacgctttc gagtcggatc
     6721 atct
//
LOCUS       DATDOZ010000125         7753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1930329, whole genome shotgun sequence.
ACCESSION   DATDOZ010000125 DATDOZ010000000
VERSION     DATDOZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930329"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..420
                     /locus_tag="VI541_02385"
     CDS             220..420
                     /locus_tag="VI541_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69786.1"
                     /translation="MTVPVPAAGGAVRVPGRQSVMLQPYIMRSTSLPGASNSSATPSS
                     ITKVKPPSRNILFDAASPPSPE"
     gene            667..2625
                     /locus_tag="VI541_02390"
     CDS             667..2625
                     /locus_tag="VI541_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLF69787.1"
                     /translation="MKAYTIPPRATRPVALVDVNVLTMKSDEVTRAQTLIIDGGRITT
                     IGPRDQVRVDGMRVIDCAGRFVLPGLADMHVHVWGLNVMGQFLANGVTTVRNMWGDGY
                     HRAVERMNRAGELAGPHLMTTSPLIDGLGEQGRTIWPYSGYIDDDLAGARTLVARYAD
                     QGYDQIKAYSLLNRDQLKALGKACREHGVRLVGHCPSPLTFEESIELGMSCFEHLTNV
                     FHGHFKDGRTLDGLAGWDPATAKKILKMIIEHADMDALRHLADSMAEREVWNCVTTTV
                     WKSPVEGAEAFTDGRMRYVSPETSAWWKFRSPPDEEVRALSRPAIERRIEVLSCLREA
                     GAPVLVGTDTPNPFVVEGFSLHDELDNFVRAGFSNFEAIRIATAEAARFAGEVDEWGT
                     LEVGMRSDLVIADSDPRKDLAVLRHPWAVASNGYFFERADLDRLLAERAEAVAAAAEP
                     QTEHVEHSHQHRHMTKRAAAVVGKLCCNAMPRDGGLMIEEAYSTNQSTQKRTVYLGPR
                     REVLRYVEVRSTDAGSSTIAIDREENGYRSEFNDFDGFASTSRIDSAPLFPSEEFGFT
                     AVSEFALTSQDGQEFESLAIQSGEMSVLPAKVTHSEDGVTIEWLRRGWPTSQTYRTDE
                     AGRVTAMTESAWMQQIELTEDPPGAAEG"
     gene            complement(2629..3147)
                     /locus_tag="VI541_02395"
     CDS             complement(2629..3147)
                     /locus_tag="VI541_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF69788.1"
                     /translation="MKTESPGFTREDVEGFVTDYLGRERGTLTARLRRVLDDVDTLVS
                     DITSRTADGSDDSWNAIEVLAHMATTAHFFGRLAHMVASQREIGDLLPMLQMRDAVTE
                     EAAQQGASALVQQLRENVERTIAFVEKTGPMDLRNPFDYVGIQMTAEDLIRVPLCAHL
                     ESHVEQIREALG"
     gene            complement(3222..4094)
                     /locus_tag="VI541_02400"
     CDS             complement(3222..4094)
                     /locus_tag="VI541_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69789.1"
                     /translation="MQPTKPTQDDSLRVKAYRSGAKMARGRMETEAPLDAEALRRVVA
                     SDIVVVSGTYDHVESVLSALEMPFQLISADQLPSVVLRPEQLLVINCPGNLPPRAVGQ
                     VREFVTRGGSLFTTDWALRHVIEPAFPGFIEYNERPTADDVVRIEIRSKDNQFLRGVI
                     DGSDDPQWWLEGSSYPIRVVDTENVEVLLVSKELADKYGESPVAVTFRFGKGEVFHMI
                     SHYYLQRTELRSERHNQSAMDYASEKGFIVASEDRADFESLPLGEVESAQSSARLFAN
                     IIADKKRKSRPSAR"
     gene            4187..4732
                     /locus_tag="VI541_02405"
     CDS             4187..4732
                     /locus_tag="VI541_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLF69790.1"
                     /translation="MTKKPGEGPPEADPEYYESLGRAIQVVRTSKGMSRRELAEASEI
                     SYPYLSEIETGKKEGSSRILLKVAGVLGMKPSELLELAESLGVANSTIDSQVRLSVSD
                     QGPARNWFGSPMASESHAVMEMMDQSPARSVSSGRAFRSRQRPEDPKEQGLARVRAEL
                     HALIDTLDRARAEALIELLRK"
     gene            complement(4762..>7753)
                     /locus_tag="VI541_02410"
     CDS             complement(4762..>7753)
                     /locus_tag="VI541_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF69791.1"
                     /translation="SGPRLEVRVGLNAGEPIREEEDYFGTPVNVAKRLCDAAQGGQIL
                     ATETVRLLASSRSAHAFIAVGPLDLKGITEPVNAYQVDWEPPEATSLPLPPALLVGAR
                     SSFVGRADEMSVIASEWESVLASQARLLLVSGEPGIGKTRLAREFSVSAHAAGATVIY
                     GRSDEDVNIPYKVFVDALRFYIIGCSDYDLDSHVAPVASEIARLVPEVVERLPRTSPP
                     SPTDSDPDRYRLFEAVRRVFTSMSNATPVVLVADDLHWSDRHTLMLLRHLVRSADPMR
                     LLVIGTFRETDLARTHPLAEMLGEFRRLDSYRRIALKGLDEGGIMQLLLTRDETQPRR
                     DLARAILDASEGNPFFVEEIIKHLSETGRLSDTKMSISDLGIPEGVREVIGRRLSALS
                     KESNSILAAASVMGREFEFDALERMVELPGAEALEAVESALDHHMVVEIVGRELPTYS
                     FTHALIQETLYSELSLPRKQRLHLKAGEALEAVLGSRATVRIAEIARHYRLAGAAADP
                     TKTLSCALQAGQAAASVFAWEEAADHLKIAYELMEQIGSDASDRARLCETLGDLAYVT
                     GIDPIERLDHFERALALYEEAGNKWKAAQVHSRLGRDLSSFPDSIDLAKANEHFRKAE
                     EYMSLEEDSAPLGYMYLGRSGAALYALDPATGMEASARAMEIAERIGHQGLWASAASN
                     HAWFIGMAGRFRDCIALMDRAWRVGDEINHVFAGFLAAWQGGYWESMRYNIDAGERFI
                     ERELDKPRISQAPRQQSILRQMLVWTRAYGGRMDEVDELMHLIGKPATFSGSAAAFGR
                     GELDQVISTCSAAVGRDSTNVWNNTGFHFTMGEAQHLIGAHVDAEQSYRVAIDISSGQ
                     HLPFECIARSRLAEIMARTGSLDLAREELERVDVLMDNQEDWMGIQARRDRAAGLLAF
                     AKGDLDVGTELIERAIESFQVQGVPWDRAWAQWELGRALADAGERESAAQHLEESLIG
                     FGTIGGWVSTIARIREDLAKLQTR"
BASE COUNT         1532 a         2348 c         2370 g         1503 t
ORIGIN      
        1 cccggccgtg ggtaacgcga gcaagccatc ctccgccgcg acggatttcc gtgtcaagac
       61 gctcgttccg ggcgatgcga tatgggttcc gatgcgactc ggtgatcaga cgtattaagg
      121 ggtgaggaga tagaagcgga agaagtcgtt ttcgatcgga aggacctcga agccgctgtc
      181 cccgcctctt tcgcataacg ctctagcgtg gaggtcctca tgacggtgcc ggtcccggcc
      241 gcgggaggcg cagtcagggt acccggtaga cagagcgtga tgctccagcc gtacatcatg
      301 cgctcgacat cgcttccggg ggcctcgaat tcgtcggcga caccttcgtc catcaccaag
      361 gtcaagcctc catcgcgcaa catcctcttc gacgcggcga gtccgcccag tccggagtag
      421 aagatacgct cgaccagttg cgcgggtcgg tcggcggtca tgtcgatgct cccttcggtg
      481 ttgctccttg agactgaagg ttgtgaggat atgacgagat gagagggatg tcatcggggc
      541 tttccgaagg gaagggcaca tgcggcatgc acatctagcg ggactcgcaa gtcgctcgca
      601 ggcttctcgg gatatcgtga cttgaggtaa gtcccacttg gaggagtccg tcatgaaccc
      661 ggcccgatga aggcttacac gataccccca cgcgccaccc ggcccgtcgc actcgttgac
      721 gtcaacgtcc tgacgatgaa gtcagatgaa gtcactcggg cacagaccct gatcatcgat
      781 ggtgggcgga tcacaacgat cggcccccgc gatcaggtgc gcgtcgatgg gatgcgggtc
      841 atcgactgcg caggtcgatt cgtactccca ggtctcgctg acatgcatgt tcatgtctgg
      901 gggctcaacg tgatgggcca gttcttagcc aacggcgtga cgactgttcg caacatgtgg
      961 ggagacggat accatcgagc agtcgagaga atgaatcgcg ctggagagtt ggcgggcccc
     1021 cacctgatga ccacgagccc actcatcgat ggcctcggcg aacaaggcag gaccatctgg
     1081 ccatacagcg gctacatcga tgacgacctc gcgggcgcga ggaccctggt cgcgcgctat
     1141 gcggatcaag gctacgacca gatcaaggcc tacagtctcc tgaaccgtga tcagttgaag
     1201 gcgctgggca aggcttgtcg cgagcacggc gtgaggttgg tgggtcactg tccttcgccg
     1261 ctaaccttcg aggagtcaat cgaacttggg atgagctgct tcgagcatct caccaacgtc
     1321 ttccacggtc acttcaaaga tgggcgaaca ctggacggtc tcgcgggttg ggatcccgcg
     1381 accgcaaaga agattctcaa aatgatcatc gagcatgcgg acatggatgc gcttcgacac
     1441 ctcgcagact ccatggcgga gcgtgaggtt tggaactgtg tgacgacgac ggtgtggaag
     1501 tcgcccgtcg agggggcaga ggcgttcact gatggtcgga tgcgctacgt ttcgccggaa
     1561 acttctgctt ggtggaagtt cagatcccca ccagatgaag aagtgagagc attgagtcgc
     1621 cccgcgatag agcgccgcat cgaggtcttg agttgcttga gagaggcggg ggcccccgtt
     1681 ctcgtaggca cagacacacc caaccccttc gtggtcgagg gattctcgct ccatgacgag
     1741 ctcgacaact tcgtgagggc cggattttcc aacttcgagg cgatccgaat tgctacggcg
     1801 gaagcggcta gattcgccgg tgaggtcgac gaatggggga cgctggaggt ggggatgcga
     1861 agcgatcttg tcatcgctga ctcagatccg cgcaaagacc ttgcggtgct gcgtcatcct
     1921 tgggccgtgg catctaatgg gtacttcttc gaacgcgctg acctcgatcg tctactcgcc
     1981 gaaagggcgg aggcggttgc agccgctgcc gaaccgcaga cagaacatgt cgaacactct
     2041 caccagcatc gacacatgac gaagcgcgct gcggccgtcg tcggcaagct ttgttgcaac
     2101 gccatgcctc gcgatggagg cctgatgatc gaggaggcgt attcgacgaa ccagtcgacc
     2161 cagaagcgga ccgtctatct cggcccccgg cgtgaagtcc tgcgttacgt tgaagtccgc
     2221 tcgacggatg cgggctcatc gacaatcgcg atcgatcgag aagagaatgg ttaccgatcg
     2281 gagttcaacg acttcgatgg cttcgcatcc accagtcgca tcgacagcgc tcccctgttt
     2341 cccagcgagg agttcgggtt cacggcggtc tctgagttcg cactaacatc ccaagatggg
     2401 caggagttcg aaagtctcgc catccagtcg ggtgagatgt cggtccttcc cgccaaggtc
     2461 acgcactctg aggatggcgt gacgatcgaa tggctacgca gaggttggcc aacctctcag
     2521 acctatcgca cggacgaggc cggtcgcgtc acggccatga ccgaatcggc atggatgcaa
     2581 cagatcgagc tgaccgagga cccacccgga gccgccgagg gctaagcgtt aacccagagc
     2641 ttcgcggatc tgctcgacgt ggctctctag gtgcgcgcat agcgggaccc ggatcaggtc
     2701 ttctgctgtc atctgaatcc ccacgtagtc gaaggggttg cgaagatcca tcgggccggt
     2761 cttctcgacg aatgcgatcg tccgctcaac gttctcgcgc agttgttgga caagggcgga
     2821 ggccccctgc tgggccgcct cctcggttac cgcatcgcgc atctgaagca tcggcagaag
     2881 gtcgccgatc tctctctgcg aggcgaccat atgagccagg cgaccgaaga agtgagcagt
     2941 ggtggccata tgagcaagga cctcgatcgc gttccaggag tcgtcggacc catcagcggt
     3001 cctagatgtg atatcgctga ccagcgtgtc cacatcatcg aggacgcgtc gaagcctggc
     3061 cgtcaaggta cctcgctcac gccccaggta gtcggtgacg aatccctcaa catcttcacg
     3121 cgtgaaacct gggctctccg tcttcatgtc tgtctccttc gcgtggttat ccccgaatac
     3181 tagtccgcat caagcgggct cagtaagacc aagggggcca gctaccgcgc ggagggacgg
     3241 ctcttgcgct tcttgtcggc gatgatgttg gcgaagagcc gggcggacga ttgagcggac
     3301 tcgacctcac cgagcgggag tgactcgaag tctgcccgat cctcgcttgc cacgatgaac
     3361 cccttctcgc tcgcgtaatc catcgcgctc tggttgtggc gctcgcttcg aagctcggtt
     3421 cgctgcaggt agtagtggga gatcatgtgg aagacctctc ccttgccgaa gcgaaacgtc
     3481 acggcaacgg ggctctctcc atacttgtcg gccagctcct tgctgacaag aagaacctcg
     3541 acattctcgg tgtcgacgac acggatcggg tacgaggacc cctcgagcca ccactgcgga
     3601 tcgtcggaac catcgatgac gcccctcagg aactggttgt ccttcgaacg gatctcgata
     3661 cgaacgacat cgtcggcggt cgggcgttcg ttgtactcga tgaagccggg gaaagcgggc
     3721 tcgatcacgt ggcgcagcgc ccagtcggtc gtgaagaggc tccctcctct ggttacgaac
     3781 tcacgcacct gaccgacggc ccgcggcgga aggttgccgg ggcagttgat cacgagcagc
     3841 tgttcggggc gcagaacaac gctcggcagt tgatcggcgc tgatcagttg gaatggcatc
     3901 tcgagtgcgc ttagtactga ctcgacgtgg tcgtaggtcc cgctgaccac cacgatgtcg
     3961 gacgcgacga ctcgtcggag agcctcggca tcgaggggag cctcggtctc catacggccc
     4021 ctggccatct tggcgccgga gcggtacgcc tttacgcgga gagagtcgtc ttgggtaggt
     4081 ttggtgggct gcatgattcg atatcctttc gctagttgtt gatattatta tgctatgagc
     4141 agagtcttgt caactgttag catccgtttc attgtaaggt gagggtatga caaaaaagcc
     4201 cggagagggc cccccagagg cggatcctga atattacgag tcgcttggcc gagccatcca
     4261 ggtggttcgg acatctaagg ggatgtcccg ccgagagctt gcggaggcgt ccgagatctc
     4321 ttacccctac ctatccgaga tcgagaccgg gaagaaggag ggctcttcga ggatcctgct
     4381 gaaagtggcg ggggtccttg gtatgaagcc gagcgagctg cttgagctgg ccgagtcgct
     4441 cggtgttgcg aactccacca tcgacagcca agtccgactt tccgtcagcg atcaaggccc
     4501 cgcccgaaac tggttcggtt caccgatggc ctcggagtcg catgccgtca tggagatgat
     4561 ggaccagtcg ccggcacgaa gtgtgagcag tggtcgagca ttccgaagtc gccaacgccc
     4621 ggaagacccc aaggagcagg ggctcgccag ggttcgggcc gagcttcatg ccctcatcga
     4681 cacactcgat cgagcaaggg ccgaggccct catcgaactc ttacgaaagt aaccgacccc
     4741 ggccctctcg ttctcaggca gtcagcgcgt ttgcagtttc gcgaggtcct ctctgatccg
     4801 ggcgatcgtc gacacccacc caccgatcgt gccgaatccg atcaaagact cctcgagatg
     4861 ctgcgccgcc gactctcgct cccccgcatc ggccagggcc cgccccagct cccactgagc
     4921 ccaggcccta tcccacggaa caccctgcac ctggaagctt tcgatggctc tctcgatgag
     4981 ttctgtccct acgtcgaggt cccccttcgc gaaagcgagt aggccagccg cccggtcgcg
     5041 acgcgcttgg atccccatcc aatcttcttg gttgtccatc aggacatcca cgcgctccaa
     5101 ctcttcacgc gcgaggtcaa gggagcctgt tctggccatg atctctgcca acctcgacct
     5161 ggctatgcac tcgaacggaa gatgttgacc cgagctgatg tcgatggcga cgcgatagct
     5221 ctgctcggca tcaacatgag cgccgatcag atgctgagcc tcccccatgg tgaagtgaaa
     5281 gccagtgttg ttccagacgt tggtcgagtc gcgacctact gccgcactac atgtcgagat
     5341 gacttgatct aactcccccc gtccaaatgc cgcggcagat cccgagaagg tcgcgggctt
     5401 cccgatcaag tgcatcaact catcgacctc gtccatacgg cccccatatg cgcgggtcca
     5461 caccaacatc tgtcgaagga tgctctgctg acgcggagct tgagatatgc gcggcttgtc
     5521 gagttcacgt tcgataaatc tctcgccagc gtcgatgttg taccgcatgg actcccaata
     5581 gcctccttgc caggccgcta ggaatccggc gaagacgtga ttgatctcgt ctcccacgcg
     5641 ccaggcccgg tccatgagtg cgatgcagtc gcggaatcgt ccggccatcc cgatgaacca
     5701 ggcgtgattg gaggcagcgc tggcccacaa cccttggtgg cctatcctct cggcgatctc
     5761 catggcgcgg gccgaggcct ccatccccgt tgcaggatca agtgcgtaaa gagccgcgcc
     5821 ggagcggccg agatacatgt aaccgagggg ggccgagtcc tcttccagag acatgtactc
     5881 ctctgccttg cgaaagtgtt cattcgcttt cgccagatcg atcgagtcgg ggaacgacga
     5941 gaggtcgcgg cccaaccgcg agtggacctg ggcggccttc cacttgttgc ccgcctcctc
     6001 gtaaagagcg agcgccctct cgaagtggtc gagcctttcg atcggatcga tgccggtgac
     6061 ataggctagg tccccgagag tctcgcagag gcgtgctcga tcagacgcat ctgatccgat
     6121 ctgttccatc agttcatacg cgatcttgag gtgatctgcc gcctcctccc aagcgaatac
     6181 actggccgcc gcctgtcccg cttggagcgc gcacgagagt gtcttcgtag gatcagccgc
     6241 cgcaccagcc agccggtagt ggcgcgcgat ctcggcgatg cggaccgtgg cgcgagaacc
     6301 aagcaccgct tcgagggcct cacctgcctt gagatgcagt cgctgtttgc gcgggaggct
     6361 gagctcggag tagagagtct cctgtatcag cgcgtgggtg aatgagtagg tcggcagttc
     6421 tctgccgacg atctcgacaa ccatgtggtg gtcgagtgca ctctcaaccg cctcgagtgc
     6481 ttctgcgccg ggcagctcga ccatccgctc tagcgcatcg aactcgaact cccgacccat
     6541 caccgacgcg gccgcgagga tcgagttgga ctccttggac agagcagaca gacgtcgccc
     6601 gatcacctct ctaacgccct ctggtatgcc gagatcgctg atcgacatct tggtatcgga
     6661 gagtctcccc gtttcagaga ggtgcttgat gatctcctcg acgaagaaag ggttaccttc
     6721 tgaagcgtcg aggatagccc gcgcaaggtc tctacgtggc tgcgtttcat cacgcgtcaa
     6781 gagaagttgc atgatgcccc cctcgtcgag tcccttcaag gcgatccggc gatagctatc
     6841 gagccgccgg aactcaccga gcatctccgc gagtgggtgc gtgcgtgcga ggtcggtctc
     6901 gcggaaggtt ccgatcacga gcaaccgcat cggatcggcc gaacgaacga gatgcctcag
     6961 cagcatgagg gtgtgacgat cggaccaatg caggtcatcc gcgaccaaca cgacaggagt
     7021 agcgttggac atcgaagtga acaccctgcg aacggcttcg aagaggcgat agcggtcggg
     7081 atccgaatcg gttggagatg gcggcgaggt ccgggggagt ctctccacga cttccgggac
     7141 aagtcgagcg atctcactcg caacgggggc cacatgcgaa tcgagatcgt agtcggaaca
     7201 gccgatgatg tagaaacgca gcgcgtcgac gaagaccttg tacgggatgt tcacgtcctc
     7261 gtctgatcgt ccatagatga cagtggcccc cgccgcgtgc gccgacaccg agaactcgcg
     7321 ggcgaggcgc gtcttgccga tccccggttc gccggagacg aggaggagcc tggcctgcga
     7381 ggcgagcacc gattcccact ctgatgcgat gaccgacatc tcatcggcgc gaccgacgaa
     7441 cgatgagcgc gcacctacca agagcgccgg cggaaggggc agactcgtcg cctccggtgg
     7501 ttcccaatcg acctggtagg cattcaccgg ctccgttatc cccttgagat cgaggggccc
     7561 cactgcgatg aacgcgtgcg ccgacctcga agatgcgaga agacgaacgg tttccgtagc
     7621 gaggatctgt ccgccctgag cggcgtcgca caagcgcttg gcgacattca ccggcgtgcc
     7681 gaagtaatcc tcctcctctc gtatcggctc gccagcgttc aacccaactc gaacctcgag
     7741 tcgggggccc gat
//
LOCUS       DATDOZ010000126         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3261664, whole genome shotgun sequence.
ACCESSION   DATDOZ010000126 DATDOZ010000000
VERSION     DATDOZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3261664"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VI541_02415"
     CDS             complement(<1..297)
                     /locus_tag="VI541_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HLF69792.1"
                     /translation="MALLYLITPCVPGPLTLQELLMQVLPAGVDLVQIRDKDANRRQV
                     EKVTSQILPLIRESGALLIVNDHVEVASAVGADGVHLGQEDMPVAQARRLLPAGG"
     gene            583..1926
                     /locus_tag="VI541_02420"
     CDS             583..1926
                     /locus_tag="VI541_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69793.1"
                     /translation="MKRFFAPISAAVLIVGILATTAFAAPAPVVKFQGSAGATALDLN
                     IPALGGLSPDLARIFPGVTIGRTSSSYGSGVAAGGAAGQCNLLAAGSGDGGDPLSLCN
                     RSTVATSDALKGNPGSDTAQCGGLFAFPATGASVVNIGTACGRSSSAITAGAPSSINE
                     ASVAFTKVGLDLSGLSPDTEVAKDALVTSVQDVLNSVFSSVPSLGIDNQQNALRSALT
                     DFLNSVSIGTHAVSIKVGPSSTIVETVGDITTVTSKAAAASVGLLGLTDALTDGLMII
                     EVSGAQASASWDNIKGIASAPAATQALATIKVRDLLDLVPGDYLTQTVTVDQLNSLLE
                     PLNTIPMLATTISVGKATSAQEGRSVSASASGIEIYALKGLGSTASANEAPNGGVDLR
                     LATAQVALAGDIALPRQDDLPRTGGPTYVYLAAGMALILGAAAAFKGARKMRATA"
BASE COUNT          406 a          644 c          594 g          389 t
ORIGIN      
        1 ccccccggcc ggcagcaagc gtcgagcctg agccacgggc atgtcttcct gaccaagatg
       61 taccccgtcc gctccgactg cggaagccac ctcgacgtga tcgttcacta tcaacagagc
      121 cccggactcc ctgataaggg gcaggatctg tgaggtcacc ttctccacct ggcggcggtt
      181 cgcatccttg tccctgatct gaacgaggtc cactcctgcg gggaggacct gcatcaacag
      241 ttcttgcagc gtaagggggc ccgggacaca tggcgtaatc agatagagaa gggccaagcg
      301 caaccgatct ccgagggaaa actcggttac tggccgtggg tttggctcac gtctagtcat
      361 aatggactac cattagagac gaaatatggc tctcaagcga tgtacggtcg ggaccctttt
      421 ccggtttctc aggcgaagct gagcacgaaa tggtgatctc tcgaaaaaat atcgcaacag
      481 ggcgtgtgct ttggggaaat ggtccgttag tactagtgaa gtaagggggg acggtccaac
      541 aatgggcgtc cacacaccta aaggaccgaa gggggtcgag ccgtgaagcg tttcttcgca
      601 ccaatctctg cagcagtctt gatcgtcggc atcttggcga cgaccgcatt cgccgcgccc
      661 gctccagttg tgaagttcca gggtagtgcg ggggcaacgg ccctcgacct gaacataccc
      721 gcgctcgggg gcctgagccc cgacctcgcc aggatattcc cgggcgtgac catcggtcgc
      781 acctcctctt cttacggctc gggagtcgca gccggtggag cggcaggaca gtgcaacctg
      841 ctggccgcag gatcaggtga cggtggagat ccactcagcc tgtgcaaccg ttcgacggtt
      901 gccacgtccg acgccctcaa gggcaacccc ggaagcgata cagcacaatg cggagggctc
      961 ttcgccttcc cggccacggg ggcctccgtc gttaacatcg gcacggcctg cggacgttca
     1021 tcctctgcga tcaccgcggg agcaccttcg agcatcaacg aagctagcgt cgcattcacc
     1081 aaggtcgggc tcgacctcag tggtcttagc cccgacaccg aggttgccaa ggacgctctc
     1141 gtcacatcgg ttcaagatgt tctcaacagc gtcttctcct cagttcctag cctcggtatc
     1201 gacaaccagc agaacgctct tcgcagtgcc ctgaccgact tcctaaactc ggtgtccatc
     1261 gggacgcacg cggtttcgat caaggtcggg ccctcgagca ccatcgtcga gactgtcggc
     1321 gatatcacga ctgtcacctc gaaggctgca gccgcttcgg ttggcctcct cggattgacg
     1381 gatgctctca ctgacggact catgatcatc gaggtttccg gtgcacaggc ctcggcatct
     1441 tgggacaaca tcaaagggat agcttcggcc cccgctgcga ctcaggccct cgccacgatc
     1501 aaggtccgcg acctgctcga cctcgtccca ggcgactacc tgacgcagac ggttacggtc
     1561 gaccagttga acagcctgct cgagcctctg aacacgatcc cgatgctggc aacgacgatc
     1621 agtgtcggca aggcaacgag tgctcaggaa ggccggtcgg tctcagcatc ggctagcggc
     1681 atcgagatct acgcactcaa aggtctgggt tcgaccgctt cagccaacga ggcccccaac
     1741 ggcggtgtcg accttcgact cgcaaccgca caggtggccc tggccggaga catcgcgctg
     1801 ccacggcagg atgacctccc ccgcacgggt ggtccgacat acgtctacct tgcggcgggc
     1861 atggctctga tccttggtgc cgctgccgcc ttcaagggag ctcgcaagat gagggctact
     1921 gcgtaacccg ccatatgccg aagttctagt cggatgaaag aaggatcctt gatggacttt
     1981 aagctctcat ggttccggac cccgactggc cgcagggttg ctgcaaccgg cct
//
LOCUS       DATDOZ010000127         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3261981, whole genome shotgun sequence.
ACCESSION   DATDOZ010000127 DATDOZ010000000
VERSION     DATDOZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3261981"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="VI541_02425"
     CDS             complement(<1..2313)
                     /locus_tag="VI541_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLF69794.1"
                     /translation="MRERVIIFDGAMGTSVHALGLALDDFWGQDGNIDVLNLSKPEAV
                     ASIHASFLDAGSDVIETNTFSSNLISQAEYDMTERVYEMNVAGAKIAREVASSYRDRP
                     RWVAGSIGPGTRSPITGQVTFDEVHDSYLLQARGLIDGGVDMLIIETCYDILQAKSAV
                     TAARTAFAQTGRKLPLIVSVTIETSGTMLWGTEIAAVVATLEPYEWIDVVGINCATGP
                     VEMTEHVRYLTRHSRRPVIVQPNAGLPTLVDGKPHFPLTPEELVRHQKVFVEEFGTAI
                     AGGCCGTTPAHIKLLAEALHGREHRARPVEFEPACASLYIAQTFKQDTSFFVIGERCN
                     TNGSRKFRELIEAQDYDACLDIAKEQVREGAHALDVCVDYVGRDGTKDITELVSRFRD
                     QSTLPLVIDSTETEVVEAALKMIGGRAVINSINLEEGEGPETRLMRNLASAKRYGAAV
                     IALAIEERGQADTAQWKLEVCRRLYDIALSNGLEPHDLIFDTLVFPISTGMEEQRRAA
                     IETLDAIAAVKKELPGSYTSLGISNVSFGLNPPARQVLNSVFLDEAVKRGLDAAIVSP
                     AKILPLARIPEERKKVALDLIYDRRTESYDPLQAFISLFEGESSGRTADPMAELMALP
                     LDERLKRRIIDGARKGLEEDLDTALQTYTALDILNDLLLDGMRVVGELFARNEMQLPF
                     VLQSAETMKRAVAHIEPHMPRVEGGGKGRIVLATVKGDVHDIGKNLVDIILSNNGYTV
                     FNLGIKQPITSIIEAAEREGADAIGLSGLLF"
BASE COUNT          430 a          714 c          715 g          458 t
ORIGIN      
        1 gaagaggagc ccggagagtc cgatcgcatc ggccccctcg cgctctgctg cctcgatgat
       61 gctggtgatc ggctgcttga tgccgaggtt gaagaccgtg taaccgttgt tcgacaggat
      121 gatgtcgacg aggttcttgc cgatgtcgtg aacatcgccc ttcacggtcg cgagaacgat
      181 ccttcccttg ccccccccct cgacgcgggg catgtgcggt tcgatatgcg caacggcccg
      241 cttcatcgtc tcagccgatt gaaggacgaa gggcagctgc atctcgttgc gcgcgaacag
      301 ttcgcccacg acccgcatcc catcgagcag caggtcattg aggatgtcga gggccgtgta
      361 ggtctgcaga gccgtgtcga ggtcctcttc gaggcccttg cgggcgccgt cgatgatccg
      421 acgcttcaga cgttcatcga ggggcagcgc catgagctcg gccatcggat cggcggtccg
      481 cccggatgac tcaccctcga agagcgatat gaaggcctga agggggtcgt agctctcggt
      541 ccttcgatcg tagatgaggt cgagagcgac cttctttctc tcctcgggta tccgagcgag
      601 cggcaggatc ttcgctggcg agacgatggc ggcatcgaga ccccgcttca cggcctcatc
      661 gaggaaaact gagttgagaa cctgacgcgc gggtggattc agaccgaacg agacgttaga
      721 tatgcccaac gatgtgtacg atccaggaag ctccttcttg actgccgcga tcgcatcgag
      781 cgtttcgatg gcggctctgc gctgctcctc cataccggta gagatcggga acaccagagt
      841 gtcgaatatc aggtcgtgag gttcgagacc gttcgacaga gcgatgtcgt agagacgcct
      901 gcagacctca agcttccact gggctgtgtc cgcctgcccg cgctcctcga tcgcaagcgc
      961 gatcaccgcg gccccatacc gtttggcgga tgcgaggtta cgcatcaggc gcgtttcggg
     1021 gccctcacct tcctcgagat tgatcgagtt gatgacggca cgccctccga tcatcttgag
     1081 ggccgcttcg acgacctcgg tttcggtcga gtcgatcacc aacggcagcg tgctctggtc
     1141 gcggaagcgt gagacaagtt cggtgatgtc cttcgtgccg tcgcgcccaa cgtagtcgac
     1201 gcagacatcg agagcgtgtg ctccctcgcg cacctgctcc ttcgcaatat cgaggcacgc
     1261 gtcatagtcc tgcgcctcga tcagctcacg gaacttacgc gatccattcg tgttacacct
     1321 ctcaccgatc acgaagaacg acgtgtcctg cttgaatgtc tgcgcgatgt agagcgatgc
     1381 acatgcgggt tcgaactcaa cagggcgcgc cctgtgttct cgcccatgga gtgcctcggc
     1441 gaggagcttg atgtgagccg gggttgtccc acagcagccg ccagcgatcg cagtgccgaa
     1501 ctcttcgacg aacaccttct ggtgccgcac caactcctcg ggcgtgagtg ggaagtgcgg
     1561 ctttccatcg accagtgtcg ggagccccgc gttcggctgc acgatgaccg gccttctcga
     1621 gtgacgcgtc agatagcgca catgctcggt catctcaacg ggaccggtcg cgcagttgat
     1681 gccgacgaca tcgatccact catatggctc cagagtcgcg accaccgcag cgatctcagt
     1741 cccccagagc atcgtcccgc tcgtctcgat cgtgaccgag acgatcaacg gaagcttgcg
     1801 accggtctgg gcgaaagcgg tcctagccgc ggtgaccgcc gacttggctt gaaggatgtc
     1861 gtagcaggtc tcgatgatga gcatgtcgac accaccgtcg atgagaccgc gggcctggag
     1921 caggtaagag tcgtgcacct cgtcgaatgt gacctgacct gttatcggcg agcgggtacc
     1981 ggggcctatc gaaccagcga cccatcgtgg gcggtccctg taggagctgg cgacctcacg
     2041 agcgatcttg gcccccgcga cgttcatctc gtagacccgc tctgtcatgt cgtactcggc
     2101 ctgtgagatg aggttcgagg agaaggtgtt cgtctcgatg acgtcagacc cagcgtcgag
     2161 gaacgaggcg tggatggagg caaccgcctc gggcttcgac aggttcagga catcgatgtt
     2221 gccgtcctgc ccccagaagt catcgagcgc aagcccgagc gcgtgcacag aagtgcccat
     2281 cgccccatcg aagatgatga cgcgctcgcg cagcgtc
//
LOCUS       DATDOZ010000128         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1931947, whole genome shotgun sequence.
ACCESSION   DATDOZ010000128 DATDOZ010000000
VERSION     DATDOZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931947"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VI541_02430"
     CDS             <1..254
                     /locus_tag="VI541_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69795.1"
                     /translation="RGAPIHDVDGFWAHRAKYGYAAVAHLQQETWIEPFWAGPRTYGY
                     EWILLDDSKKRSAWLDHKDPPRSSVDLADSFARWKASRV"
     gene            278..865
                     /locus_tag="VI541_02435"
     CDS             278..865
                     /locus_tag="VI541_02435"
                     /inference="COORDINATES: protein motif:HMM:NF042487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6642 family protein"
                     /protein_id="HLF69796.1"
                     /translation="MPRSAKPAAKRKPVPGILCLEGDWSPNLEVKQSVKPLLELLETT
                     DQMRFIYRGVNTLEEFRYLIRKWPQRQYDRYSIGYLGFHGEAGHLFFGRQSYSLEELA
                     EEIGGACQNRIIYFGSCAVLAVPRARIERFRKQTGARCVAGYTKYLDWLQSSAFDLLL
                     FDALTQYKRLDAVERKMHREYPGLVRKLGFKMFYG"
     gene            complement(891..2693)
                     /locus_tag="VI541_02440"
     CDS             complement(891..2693)
                     /locus_tag="VI541_02440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HLF69797.1"
                     /translation="MSAQTTQEVTGAEEVLWNLAELYSGPTDPAIEKDLDGALTSSRA
                     FKESYHGKVATLGAADLNAAITEMERISSIATRAGDYAYMYFSTDTSDPVRGALLQMV
                     EEKATALATELLFFTLEWVEVPEDTAARILTDPKIEAYRHFLESARKYKPYVLSEPEE
                     KILTEKSVSGSSAWSRLFTELMSAVRVQIDGVEFPMEAALSKLQQPDRGLRQRAAESI
                     TEALATGLRTRSFIFNALLLDKSTDDRLRSYPNWISSRNLSNEASDETVNALVGAVVS
                     RYDIPQRWYKLKARLLGLDRLMDYDRMAPLQEHRVATSWDEARATVIDAYESFSPKAG
                     SIIRRFFDESWIDAAAGANKTPGAYCVTTVPWGHPYVLMSFTGERRSVLTLAHELGHG
                     LHGYLAGEKNSLLNASTPLTMAETASVFGEALTFGRLLEAETDPSRRLDLLAGKLEDI
                     IATVFRQIAMNRFEDAVHTARRSSGELSVEAINEQWAATQSALFGDSVEMSEGYKTWW
                     SYIPHFIGSPGYVYAYAFGCLFSLAIFQRYQQEGASMVEPYLELLSAGGSDSPEILAK
                     LVGIDLADHNFWSGGLEAVDALLAEAEQLAQQIG"
     gene            complement(2761..3168)
                     /locus_tag="VI541_02445"
     CDS             complement(2761..3168)
                     /locus_tag="VI541_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69798.1"
                     /translation="MNRRIKHLLVATGLFLGSLFGGLALAHGGPFTVPDQDPTDLSAD
                     ISSSHAADTSEVDTSPGPSDVADDADEVEDSADDDADDADDADEVEDETDDADEVEDE
                     SGQSHDSTEPDHKGDSGSDSDSSGSDSHADNHD"
     gene            3273..3851
                     /locus_tag="VI541_02450"
     CDS             3273..3851
                     /locus_tag="VI541_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF69799.1"
                     /translation="MKAKTSALAWTPELESGVARAKEGDQQAFAEIYEVMYPAVARFL
                     YYRLGDRSAAEETAGDVFLAAWRQLSSYRGDHFPAWVFRIARNLAVGRFRTDSRARMV
                     PVEQVSDMPSPEPSPDRRAESADTNQRVRTAIRKLPDDQRDIIVMKFLLGLDNEAVGR
                     AMRKSANAVNAQQHRALRSLHEMLAKEGLSFV"
BASE COUNT          778 a         1164 c         1134 g          775 t
ORIGIN      
        1 cgcgtggggc ccccatccac gatgtcgacg gtttctgggc gcaccgcgcc aagtatggct
       61 acgccgccgt ggcgcatctc cagcaggaga cctggatcga acccttctgg gcaggtcccc
      121 gcacctacgg ttacgagtgg atcctgctcg atgacagcaa gaagcgctcc gcttggctcg
      181 accataagga cccaccccgc tcgagtgtcg atctcgctga ctccttcgcc agatggaagg
      241 catcgcgcgt ttgagcccgc gactgggggc ctgaactgtg cctcgttcgg ccaagcctgc
      301 ggcgaagcgc aagcccgtcc ctggcatcct ctgcctcgaa ggcgattggt cacccaacct
      361 cgaggtgaag cagagcgtca agcccctgct cgaactgctc gaaaccaccg atcagatgcg
      421 cttcatctac cgcggcgtta acacgctcga agagttcaga tacctgatac gcaagtggcc
      481 gcaacgacag tacgatcgat acagcatcgg gtatctcggc ttccatggcg aagcgggaca
      541 ccttttcttc gggcgccaga gttactccct cgaagaactg gccgaggaga tcggtggggc
      601 ctgtcagaat cggatcatct acttcggttc ctgcgcggtc cttgccgtcc ctcgcgcgag
      661 gatcgagcgg ttccgcaagc agacgggagc ccgatgcgtg gccggttaca cgaagtatct
      721 cgactggctc caatcgtcgg ccttcgacct attgctcttc gatgcactga ctcaatacaa
      781 gaggttggac gcggtggagc gcaagatgca ccgcgagtac ccaggcctcg tacgcaaact
      841 cggcttcaag atgttctacg gctgaatcga tgaaactcta gtccccgcga ctacccgatc
      901 tgctgggcca gctgctccgc ttcggcgagt aatgcgtcga ctgcttcgag gcccccggac
      961 cagaagttgt gatccgcgag atcgatgccg acgagcttcg ccagaatctc gggcgagtcc
     1021 gaacctccgg cgctgagcaa ctccagatag ggttcaacca tcgaagcgcc ctcctgctga
     1081 taacgctgga atatcgccag cgagaagaga catccgaagg cataggcgta gacgtaccca
     1141 ggggagccga tgaagtgtgg gatgtagctc caccaggtct tgtaaccctc ggacatctcg
     1201 accgagtcgc cgaacagagc cgattgagtt gccgcccact gctcgttgat ggcttcgacc
     1261 gagagctcgc ctgaagagcg ccttgccgta tgaacggcgt cctcgaatct gttcatcgcg
     1321 atctgccgaa agacagtagc gatgatgtct tccaacttgc ccgcgagcag gtcgagcctt
     1381 cgagaggggt cagtctcagc ctcgagtagt cgcccgaatg tcagcgcttc gccgaacacc
     1441 gacgcggtct cagccatggt cagcggagta gaagcgttga gcagcgaatt cttctcgcca
     1501 gcgaggtaac cgtgcaggcc atggcccaac tcgtgagcca gagtgagtac agaacggcgc
     1561 tcaccggtga aactcatcaa gacatatggg tgaccccacg gaacggtggt tacgcagtag
     1621 gcccccggcg tcttgttggc accagctgcc gcatcgatcc acgactcatc gaagaatcta
     1681 cggatgatcg atccagcctt cggtgagaag gactcatatg catcgatgac agtggcgcga
     1741 gcctcgtccc aacttgtggc gacgcggtgt tcttgaaggg gggccatcct gtcgtagtcc
     1801 atgagacggt ctagacccaa caagcgcgcc ttcaacttgt accagcgctg agggatgtcg
     1861 tatctcgaaa ccacggcccc caccagggcg ttcaccgtct cgtccgatgc ttcattggat
     1921 aggttgcgtg acgagatcca gttgggataa ctacgcaggc gatcatcggt cgacttgtcg
     1981 agcagaagtg cgttgaagat gaatgagcga gttctgagcc cggttgcgag ggcttcggtg
     2041 atgctttcgg ccgctcgctg acgcagacct cggtctggct gctgcaactt ggaaagagcg
     2101 gcctccatcg gaaactcgac tccatcgatc tggacgcgca ccgcgctcat cagttcggtg
     2161 aatagccgag accacgccga cgatcccgag acgctcttct ccgtgaggat cttctcttcc
     2221 ggctcactta gaacataggg cttgtacttg cgcgcagatt cgaggaagtg gcggtaagcc
     2281 tcgatcttcg ggtcggtgag gatcctggcc gccgtgtcct cgggaacctc aacccactcg
     2341 agcgtgaaga agagcagctc ggtcgcgagg gcggtcgcct tctcctcgac catctgaagg
     2401 agggctcctc taaccggatc ggacgtatca gtcgagaagt acatgtatgc gtaatcgcca
     2461 gcacgcgtcg ctatcgagga gatcctctcc atctcggtta tagcggcatt caggtcagcc
     2521 gctcctagag tcgctacctt cccgtggtag gactccttga aggcgcgcga cgacgtgagc
     2581 gcaccatcta ggtccttctc tatcgccggg tctgtgggcc ccgaataaag ttctgcaagg
     2641 ttccatagaa cctcttcagc acctgtgacc tcttgggttg tttgggcgga caaggatctc
     2701 cccttccatg ttcggaaagg gagattctac ccgcatcacc gcagctgctt tgggggcagg
     2761 tcagtcgtgg ttgtcagcgt gactgtcgga accgctcgag tccgagtccg atccactgtc
     2821 acctttatgg tcgggctcgg ttgagtcatg actctgaccc gactcgtctt cgacctcgtc
     2881 agcatcgtcc gtctcgtctt cgacctcgtc agcatcgtcg gcatcgtccg cgtcgtcgtc
     2941 cgccgagtcc tcgacctcgt cagcgtcatc agcgacatcc gagggcccag gtgacgtgtc
     3001 cacttcagac gtatcggcag catggctgga tgagatatct gctgatagat cggtgggatc
     3061 ctgatccgga acggtgaatg ggcccccgtg ggccagggcg aggccgccga atagggaacc
     3121 tagaaacagg ccggttgcca ctagcagatg cttgatcctt ctgttcatca ctacctccgt
     3181 tgtctcgttc agagagtagg tcgcgctagc cgatgaagat gtacgcaaga aaaggcgaac
     3241 atgttgaagc tcaaacgcga catatcagat agatgaaggc caagacatcg gcccttgcct
     3301 ggacccccga gcttgagtca ggggtcgcca gggccaagga gggcgaccag caggccttcg
     3361 cggagatcta tgaagtcatg tatccagcag ttgcgaggtt cctctactac cgccttggag
     3421 atcgctccgc ggccgaagag accgcgggag atgtattcct ggcggcatgg cgccaactca
     3481 gctcctatcg cggcgaccac ttccccgcat gggtgttccg gatcgctcgc aatctcgcgg
     3541 tcgggcgttt cagaacggac tctcgtgcgc gaatggttcc ggtggagcag gtttccgaca
     3601 tgccctcgcc cgagccctcg cccgatcgaa gggccgagtc agccgacacc aaccagagag
     3661 tgcggaccgc gatccgcaag ctgcccgatg accagcgcga catcatcgtc atgaagttcc
     3721 ttttaggact cgacaatgag gccgttgggc gcgcgatgcg caagtcggcc aatgctgtca
     3781 acgcacaaca gcatcgtgca ctgcggtcac ttcacgagat gctcgcgaag gaagggctga
     3841 gctttgtctg a
//
LOCUS       DATDOZ010000129         3682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_366907, whole genome shotgun sequence.
ACCESSION   DATDOZ010000129 DATDOZ010000000
VERSION     DATDOZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366907"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VI541_02455"
     CDS             complement(<1..977)
                     /locus_tag="VI541_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69800.1"
                     /translation="MPGLFTRWVTLEAHRTDSIRRPFRLGTLMSVTVLVAFTPSVALA
                     DPPSNDDFSDARAITTLPFTDQVDLTEATVEADESMGCGGEHSVWYRLETQSAGFMKI
                     STQGSGFQADFTVYQGLEVGDLVRLPDSLCSNNYRFSVSANATYFIKVEEECFNPEDQ
                     CPQGTVQISVELLPPVANDDFTSATPVTSLPFNETLNTGAATLQEGEPSECPTNDFSF
                     RSQHNTVWYRLDSPVDSVVEANTVGSDYSALIAVYTGSQMGNLSRVACAPEIARFTAT
                     LGVAYYIQIGDINVEGPGHLSLDLHRVATPANDDFLNSIEINSLPFDVVA"
     gene            1100..1171
                     /locus_tag="VI541_02460"
     tRNA            1100..1171
                     /locus_tag="VI541_02460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1132..1134,aa:Val,seq:cac)
     gene            1224..1466
                     /locus_tag="VI541_02465"
     CDS             1224..1466
                     /locus_tag="VI541_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HLF69801.1"
                     /translation="MCRSIKTLRTLEPPATAEDVEAAALQFVRKVSGYRQPSRANSDV
                     FDEAVREIADASHRLLDRLVIATERPLAKSEGPPTH"
     gene            1580..3076
                     /locus_tag="VI541_02470"
     CDS             1580..3076
                     /locus_tag="VI541_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLF69802.1"
                     /translation="MTLQSALIKSSLDAIVVVDAEGRTLVFNSAAEQTFGYSGTFVVG
                     RDLIELLFPAGQREKVRDLILASLSGAGGMVSARLIGMRADRSEIPVEVVLTLAQEEG
                     GELCLMHLRDLSGQSATEAALRDSENRFRQVFADGPVGIVLLAPDRSVISVNDAVLTM
                     LGFAEDELTSLRDITHPEDMAQDESLFERTLSGDISSYRLEKRLYTNSGEILWVNFSA
                     SLSRDREGAPLYLLGIMEDISDRVRAEEELRKSLELLRKTDEQRQRLLTRLVDAQEQE
                     RKSIAADVHDDSIQIMAATAIRLESITRHITDPDQLQALEKLKATVATSITRLRNLLF
                     ELTPTVLDHEGIRAALQQRMEMLREETGIEYRVDDGLIEEPPERVRVTIYRIVQETLA
                     NIRKHSRATEVRITLQPLNEGVFVRVKDNGIGFTFSEVEVGQPGHLGLSSVRERAQMS
                     GGWSRVISAPGSGTTVEFWLPEAQGDELKIEVAERLPEVVHIEDSGAR"
BASE COUNT          796 a         1048 c         1132 g          706 t
ORIGIN      
        1 gccacaacgt cgaagggcag actgttgatc tcgatcgagt tcaggaagtc atcgttggca
       61 ggtgtagcta ctctgtgcag atcaagcgac aagtggccag gaccttcgac gttgatatcg
      121 ccgatctgga tgtagtacgc gacaccgagc gtcgcggtaa acctcgcaat ctcgggagcg
      181 catgccaccc tcgagaggtt gcccatctgc gatccggtgt agacagcgat cagcgcggag
      241 tagtcactgc ccacggtgtt ggcctcgacg acagaatcga cgggagaatc gagtcggtac
      301 caaacggtgt tgtgctgcga tcgaaatgaa aagtcattag tggggcactc tgaaggttca
      361 ccttcttgca gggtcgcagc gccagtgttg agtgtctcgt tgaagggaag actggtgaca
      421 ggcgttgcgc tcgtgaaatc atcgttcgcg acggggggaa gcaactcaac ggagatctga
      481 acggtgccct gggggcattg atcctcagga ttgaagcact cctcttcaac cttgatgaaa
      541 tatgtggcgt tggcagacac tgagaagcgg tagttattgc tgcacagaga atcgggtagt
      601 cggacaaggt cgcctacctc caacccctga tagaccgtga aatcagcctg gaatccgctg
      661 ccctgtgtac tgatcttcat gaagcctgcg gactgcgttt ccaggcgata ccagacggag
      721 tgctctcccc cgcaccccat actttcatct gcttcaacgg ttgcttccgt gaggtcgacc
      781 tgatcagtga agggcagggt agttatcgcc ctagcatcgc tgaagtcgtc gttcgaggga
      841 gggtccgcga gcgctacact cggagtgaaa gcgacaagga ccgtcacgga catgagggtg
      901 ccgagccgga agggtcttcg aatggagtcc gtccgatgtg cctccaaggt gacccatctc
      961 gtgaaaagtc caggcatcac ttcctcccaa taacaaacac aattctgatt cgacgggccc
     1021 ggaccgattt cctgccttcg acagcaggtc gtctggcggg ttagctcttc gttaggcgct
     1081 acaattcggt ctcccctgag ggcgcgtagc tcagcgggag agcgctgctc tcacacagca
     1141 gaggtcggtg gttcgaaacc actcgcgccc acggatcctt tgttccttgg gtgtaagccc
     1201 gcatactgag gtagcgttga cccatgtgcc gcagcatcaa gacgctaagg accctcgaac
     1261 cgccagccac tgctgaggac gtcgaggcgg ccgcgctgca gttcgtccgc aaagtttcgg
     1321 ggtaccggca gccctccaga gctaattccg atgttttcga tgaggccgtc cgcgagatcg
     1381 cagatgcgtc acataggctc ctcgatcggc tcgtcatcgc gacggaacgc cccctcgcga
     1441 agtcggaggg acccccgaca cactagtcaa gaaactttct ccgaaagacg cagcgtgagc
     1501 gtggggcccc cctacacttc gatacgtgga gtcctttcgg aaggatcgtt taagcgcgcg
     1561 tcatgtatct caagagagca tgaccctaca gagcgctctc atcaaatctt cgctagatgc
     1621 gatagtcgtc gttgacgccg agggccggac cctggtcttc aactccgcgg ccgaacagac
     1681 tttcggctac tccgggacgt tcgtcgtcgg cagagacctc atagaactcc tcttcccggc
     1741 ggggcagcgc gagaaagtcc gagacctcat cttggcgtct ttgtcgggag cggggggcat
     1801 ggtgagcgct cgccttatcg ggatgagagc tgatcgcagc gagatcccag tcgaggtggt
     1861 gctcactctt gcacaggagg aagggggaga gttgtgcctc atgcacctgc gcgacttgag
     1921 tgggcagtca gccaccgagg cggctcttcg cgacagcgag aatcgcttca ggcaggtctt
     1981 cgccgatgga ccagttggga tagttctctt ggcccccgat cgcagtgtga tctctgtgaa
     2041 tgatgccgtt ctgacgatgc tcggcttcgc ggaagatgag ctcacctcgc ttcgtgatat
     2101 cacgcatccg gaagacatgg cacaagacga gtcgctcttc gagaggacct tgtcaggtga
     2161 catctcgagc tatcggctcg agaagcggct ctatacaaat tcaggcgaga tcctgtgggt
     2221 gaacttctcc gcctcgctga gtcgagaccg cgaaggagcc cccctttatc tcctcggcat
     2281 catggaggac atctccgatc gggtccgcgc agaagaagaa ctgcgcaaga gcctcgaact
     2341 gctgagaaag actgacgaac agagacaacg cctcctaacc cgactggttg atgcacagga
     2401 gcaagaacgc aagagcatcg cggcggatgt gcatgatgac tcgatccaga tcatggccgc
     2461 gactgcgatc cgcctggaga gcatcactcg acacatcacg gacccagacc aactgcaggc
     2521 gctcgagaag ctcaaggcta cagtcgcgac atccatcaca cgtcttagaa acctcctctt
     2581 cgaactgacg ccaacggtgt tggaccacga agggatccga gcggccctcc agcagaggat
     2641 ggagatgctg agggaagaga cagggatcga gtaccgagtc gatgacggat tgatcgagga
     2701 gcctcccgag cgtgtgcgcg tcacgatcta tcggatagtc caggagacgt tggcgaacat
     2761 ccgtaaacac tcccgcgcca cagaggtacg cataactctc cagccgctca acgagggagt
     2821 cttcgtcagg gtcaaagaca acggcattgg attcacattc tctgaggtcg aagtgggcca
     2881 gcccgggcat ctcgggctct ccagcgtcag agagagggcc cagatgtcgg gaggttggtc
     2941 tcgcgtcatc tcggcccccg gatcgggaac cactgtggaa ttctggcttc ccgaggcaca
     3001 gggcgacgaa ctcaagatag aggtggcaga gcggctcccc gaggtcgtcc acatcgagga
     3061 cagcggcgcg cggtaactac tcagaactcg tcaggggttg cggataggca ctcagagcgc
     3121 gcgataggac actgagacag gttagatagc ctgtcggaag ctgaagagcg accttaccga
     3181 tccttggtca ccagccgggt cgctcgtagc caatggtgaa accggccgca ggctcgacga
     3241 ggaggtgtct tcgtgcgaga gcggctccgc aagcaacttc tttggaccct ggccatcgtg
     3301 gtggcattgg ctctgcctgt tccagctatc gccctggccg gcggggcggg cgtccatgct
     3361 gactcccccg gatcgggtgg tcctccttgc gatggctgcg ttggcaacgg tgatgaccac
     3421 agtcctccgg ggcaagaaaa cggcgacgat gatcacgggt acgagtgcga tgacgactcc
     3481 gggataggtg acgggaaccc ggcccacaca ggttgcgaga gcccggatcc ctcaccgagc
     3541 gcaagcccga gcgaagagag tcctgggagt tccgatgcgg atcacggccc gccctgtgac
     3601 ggctgcgttg gcagtggcga tgatcaagat cccccaggcc aagatgaggg tgacgacaat
     3661 agcgggcacg agtgcgatga ca
//
LOCUS       DATDOZ010000130         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2732296, whole genome shotgun sequence.
ACCESSION   DATDOZ010000130 DATDOZ010000000
VERSION     DATDOZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732296"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..509
                     /locus_tag="VI541_02475"
     CDS             168..509
                     /locus_tag="VI541_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69803.1"
                     /translation="MADFSVTTTCSTCGSIPVEPTSIRLHLQDQPMSYFSCACPSCGQ
                     LLGGTVGTQVALGLAGRGATISELPFSPELLERPKCGPIDPDEMIEFHYLLERKDWFD
                     ILQRSLHPGEV"
     gene            complement(597..980)
                     /locus_tag="VI541_02480"
     CDS             complement(597..980)
                     /locus_tag="VI541_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HLF69804.1"
                     /translation="METSTTTSPSTTDTATETEVKTIGVTQAAARKISELLAAEANPA
                     YFLRVGVQPGGCSGLRYAIYFDDEEMAGDVVSTPGDFTVRVDRMSNPYLIGSTLDWLE
                     RLDASGFTVDNPNAQGSCACGESFH"
     gene            complement(1073..1252)
                     /locus_tag="VI541_02485"
     CDS             complement(1073..1252)
                     /locus_tag="VI541_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69805.1"
                     /translation="MVALILFLAAVAGAFIGFVSPLFSADAVRFRGPALKTSALLMLG
                     ALGGLGLDWAFHHLR"
     gene            complement(1289..1621)
                     /locus_tag="VI541_02490"
     CDS             complement(1289..1621)
                     /locus_tag="VI541_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69806.1"
                     /translation="MRDRSKRFEMRKLQDFLQEHEGVEGYLEPQTATLSLTLLLVARG
                     GEWARGEVSDRGQAAAFCKKVGIPFYDAAVVGYPKRLRGRKRSEAPAAPTVEELEAWF
                     ATDPHERD"
     gene            1677..>2345
                     /locus_tag="VI541_02495"
     CDS             1677..>2345
                     /locus_tag="VI541_02495"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69807.1"
                     /translation="MTTRAFPKVPWSLRKPIKANPRDHFSAEELEKSKSYQRPLQYVG
                     LAGSIISTAVGLTLVGTKIFPTWAEKSSLGWPLQLVAVIAAIILVTQVISLPLGIWAE
                     FGHERKWGFSTQTPGRYAGDQLKGLLLGVVLLSILFIPVWMLIRSTELWWVFGGLIFM
                     AFSVFVAFILPVVIMPIFNKFTPLEDESLRTRLADLATKAGVSISDYQVMDASKRTRK
                     DNAFF"
BASE COUNT          467 a          710 c          699 g          469 t
ORIGIN      
        1 cagcacttcg ctctgttcca tcggcgaatc atgtcgctcc gacctactcg gccgcaaatg
       61 agaaacggac ataactgaag acgcggcgta acgcgactgg gggccggacg ttatgacgtg
      121 tagcaatcga gggaatcaac aactcgggga aaggtggggg gacggcaatg gcagacttct
      181 cggtcacgac gacttgcagc acctgcgggt ccatccctgt cgagcctacg agcatcaggc
      241 ttcaccttca ggatcagccc atgagctact tctcatgtgc ctgcccctca tgtggacagt
      301 tgctcggcgg cacggtggga actcaggtgg cgcttggcct ggcaggcagg ggggccacta
      361 tctctgagct tccgttctcg ccagaactcc ttgagcgacc caagtgcggg ccaatcgacc
      421 cggacgagat gatcgagttc cactatctcc tggagcgcaa agattggttc gacatccttc
      481 agcgatcgct tcacccggga gaggtttgat ccagagactc agttagtgca gcggggggcg
      541 cagcggcaaa aggggaggct aacaagcctc cccttttccc atcagtatta ccccgattag
      601 tggaaagact ctccgcaggc gcatgacccc tgagcgttcg gattgtcgac ggtgaagccc
      661 gacgcatcga gacgctcgag ccaatccagc gtcgatccga tgagatacgg gttcgacatc
      721 ctgtctaccc tgacggtgaa gtcgccggga gtgctgacga cgtcgccagc catctcctcg
      781 tcgtcgaagt agatcgcgta gcgaaggccc gagcagccgc ccggctgaac gccaacgcga
      841 aggaagtacg cagggttggc ctctgccgca aggagttccg agatctttct agctgcggcc
      901 tgggtcacgc cgatggtctt gacttcagtt tcggtcgccg tatcggtcgt cgatggtgat
      961 gttgtcgtgc tggtttccac gtgggactcc tcccgatatc gatatgtgcc ccgccggatc
     1021 tggtgcgggg ttgcccttct aggataccgc gacaagcatc tgggccgcag tatcaacgca
     1081 gatggtggaa tgcccagtcg agccccaggc ccccgagcgc gcccaacatc agcagcgcag
     1141 acgtctttag agcaggcccc cggaaccgta ccgcgtccgc agagaagagc ggcgagacga
     1201 acccgatgaa ggcacccgca actgcggcaa gaaacaggat gagggcaacc acgtgtactg
     1261 actgatgaag gatcaacgat taagcggttc agtctcgctc atgtggatcg gtggcgaacc
     1321 aggcttcgag ttcctcgacc gttggcgccg cgggggcctc gctgcgcttc ctgccccgaa
     1381 gtcgcttcgg gtaaccgaca accgctgcat cgtagaacgg gatgccaacc ttcttgcaga
     1441 aagccgccgc ctggccgcga tccgagactt cgccgcgagc ccactcgccg ccccgcgcaa
     1501 ctaatagcag cgtcagcgac agtgtcgctg tctgaggctc caggtagccc tcgactccct
     1561 catgttcttg aaggaagtcc tggagtttgc gcatctcgaa tcgctttgag cggtctcgca
     1621 tcgaaccccg attgtctcat cacgcggtgc tagcattcca cgccggaggt gagggaatga
     1681 ccacacgcgc ttttccaaag gtaccctgga gtcttcgcaa gcccatcaaa gccaaccctc
     1741 gggaccactt ctcagcagaa gaactcgaga agtcgaagtc gtatcaacgg ccgcttcagt
     1801 acgttggctt ggccggttcg atcatctcga ctgcggtcgg actgaccttg gttggtacga
     1861 agatcttccc cacctgggcg gagaagtcgt cgctgggctg gccgctgcaa ctggtcgcag
     1921 tgatcgccgc gatcatccta gtgacgcagg tcatctcgct gccgcttggg atctgggccg
     1981 agttcggtca tgagcgcaag tgggggttct cgacgcagac accggggcgc tacgcgggcg
     2041 atcagctcaa aggtctgctg ctcggagttg tccttctatc gatcctgttc atacccgtct
     2101 ggatgctcat ccgttcgacg gaactctggt gggtgttcgg gggcctgatc ttcatggcat
     2161 tcagcgtctt cgttgccttc atcctgccgg tcgtgatcat gccgatcttc aacaagttca
     2221 caccgctcga agacgaatcc cttcgcacgc gactggcgga cctcgcgacc aaggcgggcg
     2281 tgagcatctc cgactaccag gtgatggatg cgagtaagcg aacacgaaag gacaacgctt
     2341 tcttc
//
LOCUS       DATDOZ010000131         3298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_367474, whole genome shotgun sequence.
ACCESSION   DATDOZ010000131 DATDOZ010000000
VERSION     DATDOZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367474"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="VI541_02500"
     CDS             complement(<1..1945)
                     /locus_tag="VI541_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLF69808.1"
                     /translation="MFKRAKALLVVVALAVPLAAQSALAQFPQKATGSDDSNPYAYQN
                     YMRLPAAQYPPSDLGGDIWKYSSKNACDLYGMISQNCDPLVNANPQELSGVTGASIDK
                     AWESTTGRPDVVIAVTDSGVKWNDLGAMDDLVNKTWLNQGELPVPDWGTPDPAPDALH
                     THDRNHDGVFNMKDYCPDWHSRNDCGGTGDLAVRGNPATPDTDYNNNGIIDPEDLIFK
                     FSNGIDNDSNGYKDDFVGWDSYEDDNDPFDEVQYGHGTGEARDSTAEINNGGDAGVCP
                     NCMVMHMRVGDSFIADASDFAQGAVYAVDNGASVIQSALGTLNNSSFAQQAIDYAYRR
                     GVVFIASAADESAAHHNQPSVLEHAVTMNSIGEPEIPGARPASYLEFRGCTNFGAYIT
                     AAVPSNSCSSEAVGRSSGMAGLAYGAGRNALALGAISDYGNLDGPGKVPAGRALSAEE
                     IHQLIATTADDINFITPVLYTARPSYPETLRYPATAGWDPYFGYGRINAKRIVDAVRL
                     AKIPPEADITSPRWFDTVDPDAGPIAIGGRVASLRSDMYSYSVRWGLWSWRDNNAAPS
                     YSTEGVTLSHVGDQTTQIEGTLATIDPAVIKSALALANGVVGAHSGPAVDPVTGRGDH
                     ENRNIPDKFGVVVQLQVVSKDGSG"
     gene            2728..2800
                     /locus_tag="VI541_02505"
     tRNA            2728..2800
                     /locus_tag="VI541_02505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2761..2763,aa:Lys,seq:ctt)
     gene            2812..2894
                     /locus_tag="VI541_02510"
     tRNA            2812..2894
                     /locus_tag="VI541_02510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2846..2848,aa:Leu,seq:tag)
     gene            complement(2896..>3298)
                     /locus_tag="VI541_02515"
     CDS             complement(2896..>3298)
                     /locus_tag="VI541_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HLF69809.1"
                     /translation="DDSGIEVDNLGGAPGVRSAYFGGPDATDEENNARLIEALDGVPV
                     AKRTARYRCVAVVVDPSGRSVEAEATCEGRIAEEPRGSGGFGYDPWFIPQGESRHMAE
                     LTLEEKQVISHRGRALRALIPLIQTLLVEKR"
BASE COUNT          609 a          982 c         1036 g          671 t
ORIGIN      
        1 tacccgagcc gtccttggag acaacctgaa gctggaccac gacgccgaac ttgtcgggga
       61 tgttgcggtt ctcatgatca ccgcggccgg tcaccggatc gacggccgga ccgctatgcg
      121 cgccgacaac gccattcgcc agcgcgagag ccgatttgat gacggccgga tcgatggttg
      181 cgagggttcc ttctatctga gttgtttggt cgcccacatg gctcagcgtc acgccctcgg
      241 tcgagtagct gggagccgcg ttgttgtcac gccatgacca gagtccccac cggaccgagt
      301 atgaatacat atccgaacgg agcgatgcga ctcggccccc gatcgcgatc ggccctgcat
      361 cgggatcgac agtgtcgaac cagcgaggcg aagttatgtc agcctcgggc gggatcttgg
      421 cgagtctcac cgcatcgacg atccgcttgg catttatgcg accgtatccg aagtatggat
      481 cccacccggc ggtcgccggg taacgcaaag tttcggggta gctgggtctc gctgtgtata
      541 gaaccggtgt gatgaagttg atgtcatcag cggtcgtagc gatgagctgg tggatctcct
      601 ccgccgagag ggcgcgaccc gcgggcacct ttccggggcc gtcgagattc ccatagtccg
      661 agatggcgcc caaggcgagc gcgttgcgcc cggccccgta ggcgagtcct gccatgccgg
      721 aactacggcc cactgcctcg gacgagcagg agttcgatgg aaccgctgcc gtgatgtacg
      781 caccgaagtt cgtgcacccg cggaactcca gataactggc ggggcgagcc cctgggatct
      841 cgggctcccc gatcgaattc atcgtcacgg catgctccaa caccgacggt tggttgtgat
      901 gggcagccga ctcatcggcg gcggaggcga tgaacacgac accccttcta tatgcatagt
      961 cgatcgcttg ctgagcgaag cttgagttgt tgagcgtgcc gagggcggac tgtatgaccg
     1021 aagcgccgtt gtcgaccgcg tagacggcgc cctgcgcgaa gtcacttgca tcggcgatga
     1081 aagagtcgcc gacccgcatg tgcatcacca tgcagttggg gcagacgcca gcgtcgccgc
     1141 cgttgttgat ctctgcggtg gagtcgcgag cctcgccggt accatgcccg tactggacct
     1201 catcgaacgg atcgttgtcg tcttcgtacg aatcccaccc gacgaagtcg tccttgtagc
     1261 cgttgctatc gttgtcgatg ccgttagaga acttgaagat gaggtcctct gggtcgatga
     1321 tgccgttgtt gttgtagtcg gtgtcggggg tcgcggggtt tcctctgacc gcgaggtcgc
     1381 cagtgccacc gcagtcattg cggctgtgcc aatccgggca gtagtccttc atgttgaaga
     1441 cgccgtcgtg gttgcggtcg tgtgtgtgca gcgcgtcggg agccgggtcg ggcgttcccc
     1501 agtcggggac cggaagttcg ccctggttca gccaagtctt gtttaccagg tcgtccatag
     1561 cgccgaggtc attccacttg acgcccgagt cggtcaccgc gatgaccaca tctgggcggc
     1621 ctgtagtgga ctcccaggct ttatcgatcg aggcccccgt gacgccgctc agttcctgcg
     1681 gattcgcgtt gacgagtgga tcacagttct gactgatcat cccgtataga tcgcatgcat
     1741 tcttcgagga gtacttccag atgtcgcccc caaggtcgct cggggggtac tgagcggcag
     1801 gcaatctcat gtagttctga tacgcgtaag ggttcgagtc atcgctgccg gttgccttct
     1861 gcgggaactg ggccagggcg ctctgggcgg cgagtgggac ggcgagagct accacgacta
     1921 agagtgcctt ggctcgtttg aacaccttgg ttcctttcgc gatcgatgac actctggtcg
     1981 gcgttgacgg cgatcctaga taacttctat gcccggatgc gaactcctgc ctcggtccag
     2041 aacgatctca gactgtcaga cctgatcctc atccttgata catggacggc tggcagaagc
     2101 gggaactcgc gcgcgatatg cggcgcaacg gcttcccact caaacggatc gctgagcagc
     2161 tctcggtctc agagtcgacc gtgtcgcggt ggagcaagga catcgagcct gggctcctga
     2221 gatcaggtgc aagatccggc agggcgcgta gggcgaagcg gcaagccgaa gagttcaaag
     2281 agattgccga tgcccgggtc gaggctcgat cacactctac cgacccattc tgggtggcag
     2341 gcttggtggc ttactgggct gaagggagga agactgccaa ctgggtctgc ttcaccaact
     2401 cagatcccgc gatggtgcga ctattcgtgg tttgggtaga gcgatatctc ggcaagggac
     2461 cggagctact gtccgccgcc ctgcaccttc actcagggat cgatgaagac gagccgaaga
     2521 cgttctggag tcgtgtcacc gacatcccaa gagacaactt cagaaggtca tacatcaagc
     2581 ctcagagcgg tggccagcgt accgagcgtc tctaccgcgg cacggcccag gtgaccatcc
     2641 agaagagttc gaaggacatg cggcggatcc gcggatggat cgagtcttac tcggaagccc
     2701 acaatcgatt cggctacact gccgccgggg ccgctagctc aactggcaga gcaaagcact
     2761 cttaatgctg aggttcgggg ttcaagtccc tggcggccca ctgcatcaat cgcgggcgtg
     2821 gcggaattgg tagacgcgca ggatttaggt tcctgtgggc aatgcctgtg gaggttcgag
     2881 tcctctcgcc cgcactcatc tcttctcgac gagaagagtc tggataagtg ggatgagagc
     2941 ccttagggct cggcccctgt ggctgatgac ctgcttctcc tcgagggtca actcggccat
     3001 gtgacgcgac tccccctgtg ggatgaacca cgggtcataa ccgaaaccac ctgaaccacg
     3061 aggttcttca gcgatccggc cctcacaagt cgcttcggcc tcgaccgacc tcccggatgg
     3121 atcgaccacc actgcaacgc atcggtagcg cgcggtcctc ttcgcgacag gcacgccgtc
     3181 gagtgcctcg atgagccgag cattgttctc ctcatcggtt gcgtcggggc ccccgaagta
     3241 tgccgatctg accccggggg ccccaccgag attgtcgacc tcgatgcccg agtcgtcc
//
LOCUS       DATDOZ010000132         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_621294, whole genome shotgun sequence.
ACCESSION   DATDOZ010000132 DATDOZ010000000
VERSION     DATDOZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621294"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /gene="prfB"
                     /locus_tag="VI541_02520"
     CDS             <1..987
                     /gene="prfB"
                     /locus_tag="VI541_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLF69810.1"
                     /translation="DLWRDPEKGRAVTQRLSQIEQTLSRVGGLSSTIDDAEVLLDLAD
                     EADDESARLEVVAELQRAEEDLSALERESLFFDEYDDHPAILSVHAGAGGVDAQDWAQ
                     MLGRMYQRYLTDAGYSVTVEDVTEGDEAGVKSLTMAVKGDHAYGVLESEKGVHRLVRI
                     SPFDSNARRHTSFAGVDVVPELAEEESVEINTDDLRIDTYRSQGAGGQHVNKTDSAVR
                     ITHLPTGLVVAVQNERSQLQNRGRAMAILKAHLRERERQERLAQIDEIRGEQGEAAWG
                     RQIRSYVMQPYQMVKDLRTDVEVGNIQRVLDGDLDPFIDGYLRWRRARYEKR"
     gene            1172..1900
                     /gene="ftsE"
                     /locus_tag="VI541_02525"
     CDS             1172..1900
                     /gene="ftsE"
                     /locus_tag="VI541_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HLF69811.1"
                     /translation="MIRLREVTKVYDGGTVAIRDVSLDIQKGEFVFLVGPSGSGKSTV
                     IRLLLRQEVVTRGTIWVAGKDITQLPAWKVPFLRRSVGTVFQDFKLLPNKTVGENVAF
                     ALEVLGRPRTVIAPQVEQVLDLVGLRDKSDRYPRQLSGGEQQRVSIARAFVNRPPILL
                     ADEPTGNLDPATSVGIMRLLDRINRSGATVVMATHDHAIVDAMQRRVVQLDDGRVIRD
                     EQAGRYETEQIPAIVFAPRAEAGP"
     gene            1957..>2543
                     /locus_tag="VI541_02530"
     CDS             1957..>2543
                     /locus_tag="VI541_02530"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HLF69812.1"
                     /translation="MVLGAVLAVFISLSLAFGALVLNQLLRINTIAWQDGVHIVAFLY
                     DEGEPGVPTGAHQALLEEVQGWNEVADAFYVDKAGAWVEYQQIFADSPERLSIDPSIL
                     PTSLRIELDDISLYRDVQFRLAAQSQLVRRINTFGEGIEQLASVSTVLNLLGLGLALV
                     LGLSAVILIANTIRMAIYARRDEVSIMKLVGASNW"
BASE COUNT          502 a          727 c          782 g          532 t
ORIGIN      
        1 gacttgtgga gggatcctga aaagggccgg gcagtcactc agcggctctc ccagatcgaa
       61 caaacccttt ccagagttgg cggcttgagc tccacgatcg atgacgccga ggtcctcctc
      121 gatctggccg acgaagcaga cgacgagagc gcccggttgg aagttgttgc cgaacttcaa
      181 cgagccgagg aggacttgtc agccctggag cgagaatcgc tcttcttcga cgagtacgac
      241 gaccaccccg ccatcctttc ggttcacgct ggggccggag gcgttgacgc tcaggattgg
      301 gcgcagatgc tgggccgcat gtaccagcgc tacctaaccg acgctgggta ttcggtcaca
      361 gtagaagacg tcaccgaggg ggacgaggca ggcgtcaaat cgctgaccat ggcggtcaag
      421 ggagatcatg catacggcgt cctcgaatcg gagaagggag tgcatcgcct tgtgcggatc
      481 agtcctttcg attccaacgc cagacgccac acgtcctttg ctggggtaga tgtcgttccg
      541 gaacttgccg aggaggaaag cgtcgagatc aataccgacg acctgcggat cgatacatat
      601 cgatcgcagg gagcgggtgg ccagcacgtc aacaagacgg attcagcggt ccgaatcacg
      661 catctcccaa caggcctcgt ggttgcggtt cagaacgagc gaagccaatt gcagaaccga
      721 gggcgggcca tggccattct caaagcgcac ctccgcgaga gagaacgtca agagcggtta
      781 gcccagatcg atgagatacg tggcgagcag ggagaggcag cctggggacg tcagatcagg
      841 agctatgtga tgcagcccta ccaaatggtg aaggacctcc gcaccgatgt cgaggtaggc
      901 aacatccagc gggtactcga cggtgatctg gatcctttca tcgacggcta cttgcgatgg
      961 cgccgagccc gctacgaaaa gagataactc ttcctctcgg acgggttcac catccccctc
     1021 cgtctcaccg cttggcagcg gcgagatgcc tcccccgtcg cttcgctccg gtggaggaac
     1081 gcgaaagttc aggttggatg tgatcgtcgg gtagccttgc cccgttctgg tcgtccaccg
     1141 accgcgccct cactcacatg tttaggcaga gatgatccga ctgagagaag tcaccaaggt
     1201 ttacgacgga ggcactgtcg cgattcggga cgtgtcccta gatattcaga agggtgagtt
     1261 cgtctttttg gtgggccctt caggctcggg aaaatcgacg gtcatcaggt tgctgcttcg
     1321 tcaggaggtg gtgacccgag gcactatttg ggtggcgggg aaggacatca cccagcttcc
     1381 ggcgtggaaa gtgccgtttc tgcgcagatc ggtcgggacc gtgttccagg atttcaagct
     1441 gcttcccaac aagactgttg gggagaatgt cgccttcgca ttggaggttc ttgggcggcc
     1501 gcgaactgtc atcgctcctc aagtggagca ggtgcttgac ctggtcggct tgcgagacaa
     1561 atcggatcgt tacccccgtc agctttccgg tggtgaacag cagcgggtat ccatcgcacg
     1621 cgcgttcgtc aatcgcccac cgatccttct tgcggacgag ccgaccggca acctcgaccc
     1681 agccacctcg gtcggcatca tgcggttgct cgatcgcatc aatcggtctg gagccaccgt
     1741 cgtcatggcc acccatgacc acgccatcgt cgatgcgatg cagcgtcgcg tagtccagct
     1801 ggacgatggc cgagttatca gggacgagca ggcgggtcgt tacgaaaccg agcagattcc
     1861 agccatcgta tttgccccca gggcggaggc cggaccgtga gtcgagccgc gttcctcatt
     1921 cgcgaggctc tgatcaacct acgaaggaac ctgcttgtgg ttctcggcgc ggtattggcg
     1981 gtgtttatct ctctttccct ggcgtttggg gccttggtgc tcaaccagct gttgcggatc
     2041 aacacaattg cgtggcagga cggggttcat atcgtcgcgt ttctgtatga cgaaggggag
     2101 cccggggttc ccactggggc ccaccaagcg ttgcttgagg aagtccaggg ttggaacgag
     2161 gtcgccgacg ccttctatgt ggataaagcc ggcgcctggg tcgaatacca gcagatcttc
     2221 gccgacagcc ctgagcgtct ttccatcgat cccagcattc tccccacctc gttgcgcatc
     2281 gagctagacg acatatcgct ctatcgggat gtgcagttcc gtctcgctgc acaatctcag
     2341 ttggtgcgaa ggatcaatac ttttggtgag ggcatcgaac agcttgcctc tgtgtcgacg
     2401 gtgctgaacc tgctcggtct gggtttggct ttagtcttgg gactgtcggc ggtcattctc
     2461 atcgccaaca ccatccgtat ggccatctac gcccgccgcg acgaggtctc gattatgaag
     2521 ttggtcgggg cctcgaactg gtt
//
LOCUS       DATDOZ010000133         6385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2212001, whole genome shotgun sequence.
ACCESSION   DATDOZ010000133 DATDOZ010000000
VERSION     DATDOZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212001"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="rodA"
                     /locus_tag="VI541_02535"
     CDS             <1..981
                     /gene="rodA"
                     /locus_tag="VI541_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HLF69813.1"
                     /translation="QFFLKRHLIYVALSCLALLVALFFDYRQLRTLAPLLFGMGILGL
                     LMVLTPLGRSALGAQRWIDLGIFQLQPSEMMKIILVIAGSSILASERAIDPAPMHVLA
                     VVGVAAAPGLLIFKQPDLGTVMVLLAIAFGILLVAGTRMRWLMLVIGVGVVSLGLAFQ
                     LGVLKDYQVARLTSFLDSQTDTQRADFNLHQSKIAIGSGGLTGKGLGRGSQTNLAYVP
                     SQHTDFIFTAIGEEVGFVGAVLVLGVFGFLLWRCIRIASLSKDRFGTLLAIGIASMLA
                     FQLFVNVGMTVGIMPITGIPLPFVSYGGSSLITNFLAVGLLMNVHMRRFT"
     gene            complement(974..1276)
                     /locus_tag="VI541_02540"
     CDS             complement(974..1276)
                     /locus_tag="VI541_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69814.1"
                     /translation="MRFDAGGRGERRLRAWVDFGLALWVADPSADSVGPSLGVGSTLA
                     GLREDASSVNAFDSSVVGGFGLRLAGRRRRLAADLGLGSSIPSVIQISSHDAGFIM"
     gene            1461..2684
                     /locus_tag="VI541_02545"
     CDS             1461..2684
                     /locus_tag="VI541_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HLF69815.1"
                     /translation="MLITAGKERIQIAILEERELIEHYVAHTHGRSIAGNIYMGRVQN
                     VLPGMEAAFLDIGEARNAVLYAGEVGFDEEVEGPTPRIETMLRSGQAVLAQVTKDPMG
                     SKGARLTTEVSIAGRFLVLVPGADFLGISRRLGDEERRRLRDIVGPLRPAPEGLIVRT
                     AAEGATAEDLERDIQRLVRIWKEVAAKSKRVKPPKLIYQEQDLAIRVMRELFTSDVQK
                     VIVDQQESFDEIKQYVEELMPDLVDRLELYQGELPLFEQHHVVEQIRKAVDRRVWVPS
                     GGHIVVDRTEAMTVIDVNTGRFVGKSTLEETVLQTNLEAAAEVAKQLRLRDIGGIIVI
                     DFIDMMQERNREELLRVLKASLSRDKTRTQVFGVSELGLVQMTRKKVSEGLIEAFSNP
                     CEVCEGRGIVLTDIS"
     gene            2786..3103
                     /gene="rplU"
                     /locus_tag="VI541_02550"
     CDS             2786..3103
                     /gene="rplU"
                     /locus_tag="VI541_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HLF69816.1"
                     /translation="MYAVIKTGGKQYRVSPGDQIEVEHLKLKSDSTTFAPLLVVTDEG
                     ETISGADNLKGYSVSAKLLGDSKGDKITVFKYRPKSGYASKTGHRQLYSLLEITSIGA
                     AQD"
     gene            3125..3376
                     /gene="rpmA"
                     /locus_tag="VI541_02555"
     CDS             3125..3376
                     /gene="rpmA"
                     /locus_tag="VI541_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLF69817.1"
                     /translation="MAHKKGASATRNGRDSNAQRLGVKVFGGQKVPAGSILVRQRGTR
                     VHPGAQVGLGKDDTLFAKAPGTVKYHERAGRKFVSIISE"
     gene            3379..4074
                     /locus_tag="VI541_02560"
     CDS             3379..4074
                     /locus_tag="VI541_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF69818.1"
                     /translation="MDPTSVAMVEAIGATRTYKTGSVEVTALSKVDVRIEAGEFVAVM
                     GPSGSGKTTLLNCLSGLDELTSGEVLVEGEPLSKMSDRERTRYRARRMGFIFQAFNLI
                     PVFSAVENVELPLLLGGRRPKVARQRALDALAEVGLSERANHRPSELSAGEQQRVAIS
                     RAIAPDPAVLWADEPTGNLDSENADKILVLLESLNRSVGLTIIMVTHDPGLGARAARL
                     VRMRDGRLLDREG"
     gene            4079..>6385
                     /locus_tag="VI541_02565"
     CDS             4079..>6385
                     /locus_tag="VI541_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLF69819.1"
                     /translation="MRALRALPTILRVAWRDFRRRPPQTVLIVAGLVVASLVISASMV
                     AADSIEDLFLENSYRAWGPVDVSVGSISGQPFPREKALAFLADPQIKSLTDGGAPRLM
                     LRAAVENLTRKTREPAVVVSGLDTLDASLGGFKFASGKGADLQGATVVIDRRLAERLE
                     ARVGDDLSFLANGLTDGEAKLNLAVVGIVEDTGKGDFRRSPNAFVDLGAMRAAVGAPD
                     VVNEILLSANGSPRSPRHPGKLEAAARRVTAQIFALPGQRPGVDVRGTKAQDLKDSVQ
                     QSKFFRAILSMLGAIVALASIALIANLFVMLGEERRSERGTMRALGLGRLELVLLGIA
                     EGVLYSAAAAIVGSFAGAFFGRYLARTMGDIFRSFTVDSAFDFATPSFALRPATLITA
                     GVAGFIVSIVSVAAVTIRTSRLTVIAAVRGLPEGRARRRRKVPWFQLFSLSLAGFLVA
                     AYFGMRTPGKAVSTGGSIVQVLGGGFLIMGVAGLLARYWQRRVGYTLGALAGLGWGLW
                     SNTFLPNFDEDPQGGFGLITMIGVMTVMAGVFLLASNLSVFRSLAVLLGGKGRVVLKT
                     ATAYADGYRFRSSMSMAMFGLVLYMIAAFAIWGGFGGGDAKTQGGGFDILATSTVPVR
                     NFDLRGATDVVPMYSTVYRFGYTVAKSQPVNFPVRVFGVDESFSKNSHFKFSAKKGRS
                     DRATWDLLAGSDDLVVLDTATNPGSAKVGDTLQMRSDSGTIRLKVAGVSDEFLLAGLF
                     VSKDTFARIFPSAAADRAWLFKAGRGVKP"
BASE COUNT         1218 a         1843 c         2020 g         1304 t
ORIGIN      
        1 cagttcttcc tgaagcgtca cctgatctat gtggcgctgt catgtttggc cctgctcgtt
       61 gcgctgtttt tcgactatcg acaactccga acccttgcac cgctgctctt cggaatgggg
      121 atcctgggtc tgctgatggt attgacaccg ttggggcgca gcgcgttggg agcccagcga
      181 tggatcgatc tggggatctt tcagcttcag ccttccgaga tgatgaagat catcctggtc
      241 atcgctggtt catcgatcct ggcatctgag agagcgatcg atccggctcc gatgcatgtg
      301 ctggcggttg tgggggtagc agccgcaccc ggccttctga tcttcaagca acccgacctg
      361 ggcaccgtga tggtgctgtt ggctatcgcg ttcgggatcc tactcgttgc tgggacccgg
      421 atgcgctggt tgatgcttgt tatcggtgtg ggagtcgtga gcctgggcct ggccttccag
      481 ctgggagtcc tgaaggatta ccaggttgct agattgacct cgttccttga ctcccagacg
      541 gatacccagc gggctgactt caatctccac cagtcgaaga tagccatagg ctctggaggt
      601 ttgaccggca aggggctggg gcgaggctcg cagaccaacc tcgcctacgt accgtcacaa
      661 catacggatt tcatcttcac ggcgatcggg gaagaggtcg gattcgttgg ggccgtgttg
      721 gttctgggcg tgttcggatt cttgttatgg aggtgcatcc ggatagcttc actttcgaag
      781 gacagattcg gcacgctcct tgcgataggc atcgcgtcga tgctcgcgtt tcaactgttc
      841 gtgaatgtag gcatgaccgt gggcatcatg cctatcaccg ggatccccct gcctttcgtc
      901 tcttacggtg gatcgtccct gatcacaaac ttcttggccg ttggtctatt gatgaatgtt
      961 catatgcgca ggttcacatg atgaaacctg cgtcatgaga ggagatctga atgactgaag
     1021 ggatcgacga acccaaaccc aagtcagccg cgagacgccg ccgtcggccc gcgagccgca
     1081 agccgaagcc acctacgacc gatgagtcga aggcgttgac cgaagaggca tcctctctga
     1141 gtccggccag tgtcgagcca actccgagtg agggcccaac gctgtcagca gaaggatctg
     1201 cgacccagag cgccaggccg aagtcaaccc aggcgcgaag gcggcgttcg ccccggccgc
     1261 ctgcatcgaa gcgcaccgag tcccagccag agtcgagcga tccgctggtc ggcgcggtgg
     1321 aaggcggcgc atcggcagag gaagccggat cgggggagtc ctcgagccag gaaagcgccg
     1381 ggtctcggcc tcgtaggaag aggcgaagcg gacgccggcc tcgcaccggt gaaccggcgc
     1441 ccccatcagg acctctgacc atgctcatca cggcgggcaa ggaacggatc cagatcgcga
     1501 tcctcgaaga gagggaactg atcgaacact atgtggccca cactcacggc cgttccatcg
     1561 ccggcaacat ctacatgggt cgggtgcaga acgtcttgcc ggggatggag gccgcattcc
     1621 tcgacatcgg tgaggcgcgt aacgcagtcc tctacgccgg agaggttgga ttcgacgaag
     1681 aggtcgaagg gccaactccg cgtatcgaga ccatgctgcg gtccggccag gctgtgctcg
     1741 cccaggtcac caaggacccg atgggttcga agggggcacg actcacaacc gaggtttcga
     1801 tcgcgggcag gttcctcgtt ctagtaccgg gtgccgactt cctggggatc agcagaagac
     1861 tcggtgacga ggaacgcaga cggctgaggg acatcgttgg ccctctgcgt ccggcccccg
     1921 agggcctcat cgtcaggacc gcagccgagg gtgcgacggc cgaggatctc gaacgggaca
     1981 tccaacggct ggtgcggatc tggaaggaag ttgccgcgaa gagcaagcgg gtcaagccgc
     2041 ctaagctcat ctatcaagag caggaccttg cgatccgagt gatgcgcgaa ctgttcacga
     2101 gcgacgttca gaaggtcatc gtcgatcagc aggagagctt cgacgagatc aaacagtacg
     2161 tcgaagagtt gatgcctgac ctggtcgacc gtcttgagct ttatcagggg gagttgcctc
     2221 tattcgagca gcatcacgtc gtcgaacaga tccgcaaggc ggtcgatagg agggtctggg
     2281 ttccctcggg cgggcacatc gtggtcgatc ggacagaggc catgacggtc atcgacgtga
     2341 acaccggacg tttcgtcggc aagtccacgc tggaggagac cgtccttcag accaatctcg
     2401 aagcggctgc cgaggtcgcc aagcagctga gactgaggga catcgggggg atcatcgtca
     2461 tcgatttcat cgacatgatg caggagcgca accgtgagga actgctaagg gtcctgaagg
     2521 cttcgttgag ccgtgacaag accagaactc aggtcttcgg cgtctccgaa cttggcctcg
     2581 tacagatgac tcgcaagaag gtctcggagg ggctcatcga ggctttctca aacccctgcg
     2641 aagtgtgcga gggcaggggc atcgtcctta ctgatatctc atgaccagcg caacccctga
     2701 tatcatttga catccacccc tcgttcgagg gaggcacccc caccgggccg gttgtgggta
     2761 acgaaggaaa gacgagggtt tcaccatgta tgcagtgatc aagaccggag gcaagcagta
     2821 tcgggtttcg cccggtgacc agatagaagt ggaacatctc aagttgaaga gtgattcgac
     2881 caccttcgcg ccacttctgg tcgtgaccga tgagggcgag accatctcgg gggccgataa
     2941 tcttaagggc tactccgtca gtgcgaaact gctcggtgat tccaaaggcg acaagatcac
     3001 cgtcttcaag taccgcccca agtcgggcta tgcatcgaag accggacacc gccagctcta
     3061 ctcgctgctg gagatcacct cgataggcgc ggcccaggat tagggctggt atccttcatc
     3121 aaccatggct cacaagaaag gcgcttccgc cacacgcaac ggccgcgact cgaacgctca
     3181 gcggctcggc gtgaaggtat tcggagggca gaaggttccg gcaggttcga tcctcgttag
     3241 gcagaggggc acccgcgtac atcctggagc gcaggttggt ctcggtaaag acgacactct
     3301 cttcgctaag gcccccggta ccgtcaagta tcacgagcgg gccgggcgga agttcgtaag
     3361 catcatcagc gaatagtttt ggaccccact tcggtggcga tggtcgaagc catcggcgcg
     3421 acccgcacat acaagaccgg ctccgtagag gtcaccgctc tcagcaaggt tgatgtgcgc
     3481 atcgaggccg gtgagttcgt tgcagtgatg ggtccctcgg gtagtggaaa gaccacgctg
     3541 ctcaactgcc tctccggtct cgatgaactc acctcgggcg aggtgctggt cgaaggtgaa
     3601 cccctttcca agatgtctga ccgcgaacga acccgctatc gcgctcgccg tatgggcttc
     3661 atcttccagg ccttcaacct catcccggtg ttctcggcgg tcgaaaacgt cgagcttcca
     3721 ctactactcg gagggcgtcg ccccaaggtg gcccgccaac gtgcgttgga tgctctcgcc
     3781 gaggtcggtc tatccgagcg ggccaatcac cgcccttccg aactgtcagc gggggagcag
     3841 cagcgggtgg cgatctcgag agccatcgcc cccgacccag cggtgttatg ggcagacgaa
     3901 ccgaccggga acctcgactc cgagaatgcg gacaagatcc tggtgctcct cgaatccctc
     3961 aaccgcagcg ttgggctaac gatcatcatg gtgacgcatg accccggcct cggtgcaagg
     4021 gcggccaggc tggtacgtat gcgagacgga cgactcctcg accgggaagg gtgagtcgat
     4081 gagggcgctg cgggcgctcc ccacgatcct gagggtcgct tggcgtgact ttcggcgaag
     4141 gcccccacag acagttctga tagttgcagg tcttgtcgtc gcctcgttgg tcatctcggc
     4201 ttctatggtt gcggccgact ccatcgagga tctcttcctc gagaactcgt atcgagcgtg
     4261 ggggcctgta gatgtatcgg tcggatccat ctcagggcag ccgtttccgc gtgagaaagc
     4321 actcgcattc ttggctgacc cgcagatcaa gtcgttgacc gacgggggag cccccagact
     4381 gatgcttcgc gccgcggtcg agaacctgac tcgcaagact cgtgagccgg ctgtcgtcgt
     4441 cagcggtctc gatacgctcg acgcgtcgct cggaggcttc aagttcgcgt caggcaaggg
     4501 ggccgacctt caaggtgcga ctgtcgtcat cgataggcgt ctcgcagaac gtctcgaagc
     4561 gcgggttggt gacgatctct cattcctcgc caacggtctc actgatgggg aggcgaaact
     4621 gaacctcgcg gtcgtgggta tcgtcgagga caccggcaag ggcgactttc gacgaagccc
     4681 caatgctttc gttgacctgg gcgcgatgcg ggcagcggtt ggggcccccg atgttgtcaa
     4741 cgagatactc cttagcgcca atggctcgcc ccgcagcccc cgacatcctg ggaagctcga
     4801 ggccgccgcg cgaagagtga cagcgcagat attcgccctt cccgggcaaa gacctggcgt
     4861 agatgtccgt ggtacgaagg ctcaggacct aaaggattcg gttcagcaga gcaagttctt
     4921 cagggccatc cttagcatgc tcggcgcgat cgtggccctc gcatcgatcg ctctgatcgc
     4981 caacctattc gtgatgctcg gtgaggaacg acggtccgag cgcggaacga tgcgagctct
     5041 gggccttggt cgcttggagt tggtgctgct cgggatcgcc gaaggggtcc tctattcggc
     5101 ggcggccgca atcgttggct cgttcgctgg tgcgttcttc ggaaggtacc tggccaggac
     5161 gatgggggac atcttccgaa gtttcacggt ggactccgcc tttgacttcg caactccctc
     5221 gttcgcgctc aggccagcaa cattgataac tgccggtgtc gcaggtttca tcgtctcgat
     5281 agtttcggtc gcagccgtca ctatcagaac gagccgcttg acggttatcg ctgccgtccg
     5341 cgggctgccc gaagggcggg caaggcgacg caggaaggtc ccctggttcc agcttttctc
     5401 cctgagcctt gcgggttttc tcgtagctgc ttacttcggg atgaggaccc ccgggaaagc
     5461 cgtctccacc ggcggctcta tcgtgcaggt gctcggtgga ggctttctga tcatgggtgt
     5521 cgcaggactg ttggcgcgct attggcaacg acgagtcggc tacacattgg gtgcattggc
     5581 tgggctcggg tggggcttgt ggagcaatac attcctgccg aacttcgatg aggaccccca
     5641 gggcgggttc gggttgatca ccatgatcgg ggtcatgacg gtgatggccg gggtcttcct
     5701 tcttgcatcg aacctctcgg tcttccgaag cctcgccgtg cttctagggg gcaagggcag
     5761 ggtagttctt aagacggcga ccgcctacgc agatggttat cgcttcagga gttccatgtc
     5821 catggcgatg ttcggcttgg tcctctacat gatcgcggca ttcgccatat ggggaggctt
     5881 cggtggtgga gacgcgaaga cccagggcgg tgggttcgac atcctggcga catcgacggt
     5941 gccggtgcgc aacttcgacc tgcggggcgc gaccgatgtc gtgccgatgt attcaacggt
     6001 ctacaggttc ggctacacgg tggccaagtc tcaacccgtc aacttcccag ttcgcgtttt
     6061 cggcgtcgat gagagtttct ctaagaactc gcacttcaag ttttccgcga agaagggccg
     6121 atccgatcgc gccacttggg atctgctcgc tgggtcggat gaccttgtcg ttctcgacac
     6181 cgccaccaac cctggatcgg ccaaggttgg agacaccctg cagatgaggt cggactccgg
     6241 aacgatccgg ctcaaagtgg ccggggtgag tgatgagttt ctgctggcgg gtctcttcgt
     6301 ctctaaggac acatttgctc gcatctttcc tagtgcggcc gcagacaggg cgtggctttt
     6361 caaggcgggc cgaggggtga agccg
//
LOCUS       DATDOZ010000134         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2733578, whole genome shotgun sequence.
ACCESSION   DATDOZ010000134 DATDOZ010000000
VERSION     DATDOZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733578"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VI541_02570"
     CDS             <1..552
                     /locus_tag="VI541_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HLF69820.1"
                     /translation="VRAIDVVAFVPLDQIDPAYFQKPYYLAPEATGIKAYKLLEEAMR
                     QSGKVGIAKITLREKEHLATLRVANGVFVLETMHWPDEIRAAEFEELTRTPEIRPAEL
                     ELALNIIETLSDDFHPEEFVDSYRERLEAAVRSKVDGQEIAVTPTKEPTQVLDLMEAL
                     KASVEQARQRADVRQEVREAALN"
     gene            complement(545..1471)
                     /gene="ligD"
                     /locus_tag="VI541_02575"
     CDS             complement(545..1471)
                     /gene="ligD"
                     /locus_tag="VI541_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HLF69821.1"
                     /translation="MLASTRDQAFDSDDFLYEVKWDGMRILAFCERGKTRLYSRSRRE
                     VTHQFPEFAGRHESLRADAVLDCEIVAMDETGKPSFERLQSRIGLSKPGDVARGVADV
                     SLDLICFDLLHMDGGWLGDRPLTDRLGLLESEVAFGGRVLRSDVVEGQGVALFEAVAA
                     KGLEGIVAKRRSSRYLPGTRSKEWFKVKTSRSIDCVIGGWKPSAGHRDEVVGSLLVGA
                     YDDSGLRYLGSVGTGFDEKTRHELTSLLRAIGSVQNPFSEKIDPGAVNFVEPQLVCTV
                     EYRELTRAKKLRAPSFKGLRTDKAPAQCLLIS"
     gene            complement(1513..1881)
                     /locus_tag="VI541_02580"
     CDS             complement(1513..1881)
                     /locus_tag="VI541_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLF69822.1"
                     /translation="MYNKILIATDGSPTAQKAAARGSELARDVGAEVIYLSVGDPAEK
                     LPVAQQAAETFGEGLTVTPLAVEGHPADVICAVAQERGVDLIVVGNRGMTETKLLLLG
                     NVPNQVSHHAPCNLLIVKTT"
     gene            complement(1883..2359)
                     /locus_tag="VI541_02585"
     CDS             complement(1883..2359)
                     /locus_tag="VI541_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLF69823.1"
                     /translation="MSARAEVAVGYRKVMVGVDGSETAKGALMEAAQIAAREGSELLI
                     VSSYEPPDIRQLERWQAESPQEVSWRLTGTTLVEEVLRSASQLVRKEVALDPKTRFEE
                     GDPAEVLITVAQEEGVDLIVVGNKGMTGAKRFLLGSVPNKISHHSPCDLLIVKTTD"
BASE COUNT          496 a          848 c          723 g          468 t
ORIGIN      
        1 gtcagagcga tcgacgtcgt ggccttcgtg ccactcgatc agatcgaccc ggcctacttc
       61 cagaagccct actatctggc ccccgaagcc accggcatca aggcctacaa gctgctggaa
      121 gaggccatgc ggcagtcggg caaggtcggc atcgccaaga tcacccttcg cgagaaggaa
      181 cacctcgcaa cgctgcgcgt cgccaacggc gtcttcgtac tcgagaccat gcactggcct
      241 gacgagatac gggccgccga gttcgaggag ctgaccagga cccccgagat acgccccgcc
      301 gaactcgaac tcgctctcaa catcatcgag acactctccg acgacttcca ccccgaggag
      361 ttcgtcgatt catatcgcga gcgcctagaa gcagcggtcc gctccaaggt agatgggcag
      421 gagatcgccg ttaccccaac gaaggagccc acacaggtcc tcgatctgat ggaggcgctc
      481 aaggcaagcg tcgaacaggc ccgtcaaagg gcagacgttc gccaggaggt ccgcgaagcc
      541 gctctcaact gatcagcaga cactgcgcgg gggccttgtc cgttcggagc cccttgaacg
      601 aaggcgctct cagcttctta gcgcgagtaa gttctcgata ctcgaccgtg cagacgagtt
      661 gaggctcgac gaagttcacg gcccccgggt cgatcttctc tgagaagggg ttctgcaccg
      721 aaccgatggc gcgaagaaga gaggtcagct catgccgagt cttctcgtcg aaccccgtgc
      781 cgaccgagcc gagataacga aggcccgagt cgtcataggc cccgaccagc agactgccaa
      841 cgacctcgtc gcgatgtccc gcgctcggct tccaacctcc gatcacgcag tcgatgctgc
      901 gcgaggtctt gaccttgaac cactccttgc tccgagtacc ggggagatac ctgctcgacc
      961 ggcgcttggc gacgatcccc tccaacccct tggccgcgac cgcctcgaag agagcaacac
     1021 cttgaccctc gaccacatca gagcgcagga cccggcctcc gaaggcaacc tcgctctcga
     1081 gtagaccgag gcgatcggtg aggggacggt ctccgagcca cccaccatcc atgtggagaa
     1141 gatcgaagca gatcagatca agtgagacat cagcgacccc gcgcgccaca tccccgggct
     1201 tgctgaggcc gatcctgctc tggagccgtt cgaaagatgg cttccccgtt tcatccatcg
     1261 caacgatctc gcagtcgagg accgcgtccg ctcgcaagct ctcatgtcgt ccagcgaact
     1321 caggaaattg gtgagtcacc tctcgccgtg agcgcgagta caggcgggtc tttccgcgct
     1381 cgcagaatgc gaggatgcgc atcccatccc atttgacctc gtagaggaag tcatctgagt
     1441 cgaaggcttg atcacgcgtc gaagccagca tcgggacgaa aacatccggg agccccgaag
     1501 gcaatctggc tgctaggtcg tcttgactat cagcaggttg cagggggcat ggtgcgagac
     1561 ctggttcggc acgttgccta gaagcagcaa cttggtctcg gtcatcccgc ggttgccgac
     1621 cacgatgagg tcgacgccgc gctcctgtgc caccgcgcag atgacatcgg ccgggtgacc
     1681 ctctacagcg aggggcgtaa cggtgaggcc ctcgccaaat gtctcagcgg cttgctgcgc
     1741 gaccgggagt ttctcggcgg ggtccccaac gctcaagtag atgacctcgg cccccacgtc
     1801 gcgtgcgagt tccgaccccc tcgctgcggc cttctgtgcg gtaggtgagc catcagtagc
     1861 gatcaggatc ttgttgtaca tatcagtcgg ttgtcttgac tatcagaagg tcgcacggtg
     1921 agtggtgcga gatcttgttc ggcacgcttc cgaggaggaa acgcttggca ccggtcatgc
     1981 ccttgttccc cacgacgatg aggtcgacgc cttcctcctg cgcgaccgtg atcaacacct
     2041 cggccgggtc gccctcctcg aagcgcgtct ttgggtcgag cgcgacctcc ttgcgcacaa
     2101 gttggcttgc cgacctcagc acctcttcca cgagtgtcgt tccggtcagc cgccaactga
     2161 cctcctgcgg tgactcggcc tgccatcgct ccaactggcg gatatccggt ggctcgtacg
     2221 acgagacgat cagcagttcc gatccctcgc gggctgcgat ctgggctgcc tccatgaggg
     2281 ccccctttgc cgtctcggag ccatcgaccc caaccatcac cttgcgataa cccacggcca
     2341 cctccgcgcg agcggacact gaaccttctt catatcacaa cccaactctg tcgaatcact
     2401 cgatcttcgc ggcggcttgc atcgcgagat ctggttcgtc agtgatgatg ccaccgcacc
     2461 cccactcgat cagttgttta atccggtccg agtcgttcac cgtccatgcg atgagttcgc
     2521 atcccgccgc cgatg
//
LOCUS       DATDOZ010000135         7820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1687363, whole genome shotgun sequence.
ACCESSION   DATDOZ010000135 DATDOZ010000000
VERSION     DATDOZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687363"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VI541_02590"
     CDS             complement(<1..711)
                     /locus_tag="VI541_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HLF69824.1"
                     /translation="MGETLQEIFSNSYVLLGLKLGVLLILLPATLVLGWVELKLGAHM
                     QARLGPMEAGGFHGWAQTLADGIKFVLKEDIIPDAADRKVFSLVPFITMLGASLTFVV
                     IPIGPKLIIEDLDVGIFFVLAASSISVIGVLMAGWSSANKYSLMGALRAAAQLMAYEL
                     PLVLVAAGIAMQAGTLSLVGIVEAQRNYLHFGLPYIVGPGLIGFLIFLTAGTAELTRP
                     PFDMPVAEAELITGAFTEY"
     gene            complement(711..1856)
                     /locus_tag="VI541_02595"
     CDS             complement(711..1856)
                     /locus_tag="VI541_02595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HLF69825.1"
                     /translation="MDDVPGTTSARLSGSGMLHTEEMVLNIGPQHPATHGVLRVRVTL
                     DGEKIIDGEPIIGYMHRGHEKLLEQRDFRQITNLLSRMDWLSGINNEIPASIAVEKLM
                     EIEVPRRAQYLRVILAELNRITNHLMFVGSFGLELGATTPVMYAFREREIIQTAIEMV
                     TGGRMHFGFIRPGGLKEDVPRGFVEAALRGVRELAKKIPDYEGILMGNEIYKQRTVGI
                     GVLPPDLALEHGVSGPLLRGSGVAYDVRRADPYLIYDEFDFDIPVGSSGDAFDRYFVL
                     MEEMKESAKIVEQALEGLPAGSHIGQVPKRLTLPVGEIYSRAENPLGELGYYLISDGE
                     AKPYRMKVRTPSFSNVSVIPHMLRGLYVPDMIAVLGSMFFVVGDIDR"
     gene            complement(1856..2314)
                     /locus_tag="VI541_02600"
     CDS             complement(1856..2314)
                     /locus_tag="VI541_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLF69826.1"
                     /translation="MSETRLQYDHVVATVDPDRIVEVLRTLRDDPKFQCDYLTFLAGV
                     DRLDAGFEVIITVYSTTLGNTVILKVLLPRENPSMPTITNVYGGAHWHERECFEMFGI
                     GFDGHPNLRNLYLPDDFVGHPLLKSFRLASRTFKPWPGAKDPEEAAGGGR"
     gene            complement(2346..2798)
                     /gene="nuoB"
                     /locus_tag="VI541_02605"
     CDS             complement(2346..2798)
                     /gene="nuoB"
                     /locus_tag="VI541_02605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HLF69827.1"
                     /translation="MFQFGLACCSIEFIASAMPKHDIMRLGVIPFPASPRQADLMVVA
                     GTVTDKMAPAIKRVYEQMPEPKYVISMGSCANCGGPYWDSYSVTKGIDQILPVDVYVP
                     GCPPRPEGLLHGIVRLQQMIQGEDIPEKWRGSSKQIPQVIGTGRAGGI"
     gene            complement(2886..3254)
                     /locus_tag="VI541_02610"
     CDS             complement(2886..3254)
                     /locus_tag="VI541_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLF69828.1"
                     /translation="MPTGYYGSYLTLAIFAGAVFVLVGGTLLLNRLIAPRVRTEEKYM
                     TYECGVDPIGGGWTQSTVRYYIFALLFVIFDVEAVFLFPWATVFEKIGIFAFVEMMIF
                     IGILALGLVYAWRKKVLEWV"
     gene            complement(3501..4382)
                     /locus_tag="VI541_02615"
     CDS             complement(3501..4382)
                     /locus_tag="VI541_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HLF69829.1"
                     /translation="MATPRVVVHGGAGLMDRTRLDACLEGVEAAAKTGAESLGSGALA
                     AAVAAVGSMEANPNFNAGLGATLTRQGSVELDAAVMTGDLRFGAVGACPPIPSAIELA
                     LRVYEDGEYSLLVGEGALNFAMESGIRPLGPEDLILDRVRAQLIAETARRTNGQGISG
                     AGTVGAVALDADGNLAAATSTGGILYKRVGRVGDTPMPGAGTYADAELGGAASATGHG
                     ESILKVLLSREVVARVATSGPEIASKEAMLTFERRTQGLGGLIVIDRTGSFFAIHNTT
                     HMPWAACTPGEPPTSGH"
     gene            complement(4382..5056)
                     /locus_tag="VI541_02620"
     CDS             complement(4382..5056)
                     /locus_tag="VI541_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HLF69830.1"
                     /translation="MPRISEYESYVGTEQLFAALKPADLLVNQDERLFQVTHQTAELW
                     MKVIEHEVPYAVSKMEEGSLREASASFRRCAMIERLISRELGILETMAPANYQRIRMG
                     LGRGSGSESPGFRFLLTEVGPMVSKTLEPLLDGHRVAPFDVWMDPEGKPDLHELIAAI
                     IDFDQSFQEFRYHHLNLARREIGLDVRSLKGAPATVLIAGATEPLFPKLWDAINDVTR
                     TFKPEY"
     gene            complement(5092..6546)
                     /locus_tag="VI541_02625"
     CDS             complement(5092..6546)
                     /locus_tag="VI541_02625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLF69831.1"
                     /translation="MPDLHIQGVDLWAIAAEGVLAVGGLLLLLGGAFVRRARPGPLAL
                     AALAIVLTAAGFLVPSIGRVSRAFDNTVAVDGFAIFLKMALLATAALTILAMHGYLRS
                     EGGPIGEALGLVLFATAGMVLMTAATDLIVIFIALETFSLALYILVALRRGRADGQEG
                     SLKYFLTGAFSSAFFLYGIALVYGATGTTRLLGIARSVREVSILQQGLLVPGIALLAI
                     GLVFKISAVPFHMWTPDAYQGAPAPVAGFMAAGSKIAGFAVALRLFYVVFPSLRWDWQ
                     PILIVISVASMLLGSFAAIAQRNLKRMLAYSAIAHTGFVLIGLIANNERGVSGSAFYL
                     AVYAITIIGAFAVIYAVGGSGESLVELRDYRGLWRRQPFLAAVLAVDLISLAGIPPTA
                     GFWAKFEVFSAGVSAGLTPVVVIAVMTSAVAAFFYLRVIVAMFLEDDEAPEPASTHPS
                     LGIVMIISIVFILLIGVLPTQLLDAARHAGFAFH"
     gene            complement(6546..>7820)
                     /locus_tag="VI541_02630"
     CDS             complement(6546..>7820)
                     /locus_tag="VI541_02630"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLF69832.1"
                     /translation="RYHLGIDGISLWLVLLTTLLMPLCVLASWKVTDRVKSFHLVLLV
                     LETGILGVFLALDLLLFYVFWEGMLIPMYLLIGIWGYQRRVYAAVKFFLYTLTGSLLM
                     LVGIVSLYFLGPHTFDLTKLAQVHFAHSAQLWLFAAFFASFAIKIPIFPFHTWLPDAH
                     TEAPTAGSVLLAGVLLKMGAYGFLRFSFPLFPEAVARFAPVMVVLGVIGIVYGGLVSI
                     VQRDIKRLVAYSSVSHLGFVVLGIGVLNQIATSGSVLQMINHGLTTGALFLLIGMLYQ
                     RAHTRQISELGGVNRLMPVFGGLFLITSLASLGLPGLNGFVGEFLILNGSFTYSRPIA
                     VIATSGVVLAAIYLLWAYERVFTGPANLPHQERKSIWSDVSLREVLVVAPLVVMSFVI
                     GIFPRPFLERIEPSVARLIDNVHQPTLRLAGK"
BASE COUNT         1727 a         2356 c         2363 g         1374 t
ORIGIN      
        1 gtactcggtg aaggcccctg tgatgagttc cgcctcggcc accggcatgt cgaatggggg
       61 cctggtcagc tccgcggtac cggccgtcag gaagatcagg aaaccgatca ggccggggcc
      121 cacaatgtag ggcaagccga agtgcagata gttgcgctga gcctcgacga tgccaacgag
      181 cgacaaagtt cctgcctgca tcgcgatccc tgccgcgact aacaccagcg gaagttcgta
      241 ggccatcagc tgcgcggccg cgcgcagggc ccccatcagc gagtacttgt tggccgacga
      301 ccacccggcc atcagcacgc cgatcacgct gatcgacgac gcggccagaa cgaagaagat
      361 gccaacatcg aggtcctcga tgatgagttt cggcccgatg ggtatgacga cgaatgtgag
      421 tgaggccccc agcatcgtga tgaatgggac gagcgagaag actttacggt cggcggcatc
      481 ggggatgatg tcctctttca gaacgaactt gatgccatcg gcgagcgtct gagcccaccc
      541 gtggaaacca ccggcctcca tgggaccgag ccgagcctgc atgtgagcac cgagcttcaa
      601 ctcgacccag ccgagcacaa gcgttgctgg cagcaggatg agcaagactc ccagcttcaa
      661 gccgaggaga acgtagctat tcgagaagat ctcttgcagg gtctcaccca tcagcgatct
      721 atatctccca ctacgaagaa catgctcccg agcacagcga tcatgtcggg cacgtacaaa
      781 ccccggagca tatgcgggat gacactgaca ttggagaacg atggagtgcg aactttcatg
      841 cgatacggct tcgcctcgcc atcggagatg aggtagtaac cgagttcacc cagaggattc
      901 tcggcacgcg agtagatctc gccgacagga agagtcaaac gcttggggac ctgaccgatg
      961 tgcgagccag cggggagtcc ctcgagagcc tgctcgacga tcttggccga ctccttcatc
     1021 tcctccatca ggacgaagta ccgatcgaac gcgtctcccg atgagccaac ggggatatcg
     1081 aagtcgaact cgtcatagat gaggtaaggg tcggccctgc gaacgtcgta ggccacgccg
     1141 ctgcctcgaa gcagcggtcc agagacgccg tgctcaagag cgagatcagg tggcaaaacc
     1201 cctatgccca cggtgcgctg cttgtagatc tcgttgccca tcaggatccc ttcgtagtca
     1261 gggatcttct tcgctagctc acggactcca cgaagtgcgg cctcgacgaa gccgcgcggc
     1321 acatcctcct tgagcccccc aggtctgatg aacccgaagt gcatgcgacc cccggtgacc
     1381 atctcgatag ctgtctggat gatctcgcgc tcacggaatg cgtacatgac cggggtagtt
     1441 gcgcccagtt cgagcccgaa cgatccaacg aacatgaggt gattggtgat gcgattgagc
     1501 tcggcgagga tcactcggag gtactgcgcc ctgcggggga cctcgatctc cataagcttc
     1561 tcaaccgcta tggaggccgg gatctcgttg ttgatgcccg agagccaatc catccgcgac
     1621 agcaggttag tgatctgacg aaagtcgcgc tgttcgagga gcttctcgtg gccgcgatgc
     1681 atatagccga tgatcggctc gccatcgatg atcttctctc cgtcaagggt cacgcgaacg
     1741 cgaagcactc cgtgggtcgc agggtgctgg gggccgatgt tgaggaccat ctcctcggta
     1801 tgcagcatcc cgctgccgct caatcgggcg cttgtggttc caggcacgtc gtccatcaac
     1861 gacctccgcc ggcggcctct tcggggtctt tggcccccgg ccacggtttg aacgtacgac
     1921 tcgctaaccg aaaactcttg agcaacgggt gcccgacgaa gtcatctggc aggtagaggt
     1981 tgcgcagatt cggatgacca tcgaacccga tgccgaacat ctcgaaacac tctctctcgt
     2041 gccagtgggc acccccatag acgttcgtga tggttggcat gcttgggttc tctcgaggca
     2101 gaaggacctt gaggatcacg gtgttgccaa gagtcgtgga gtagaccgtg atgatgacct
     2161 cgaatcctgc atccaacctg tcgacgcccg ccaggaaggt caggtagtcg cattggaact
     2221 ttgggtcgtc tctgagggta cgcagaactt cgacgatccg gtctggatcg accgtcgcca
     2281 cgacgtggtc gtactggagg cgggtttcgc tcacggaatc tccgaacgaa tcggtgaccc
     2341 gctggctaaa ttcctccggc gcgaccagtg ccaatgacct gtgggatctg tttgctgctt
     2401 ccacgccact tctccggtat gtcttcgcct tggatcatct gttgtagccg aacgattccg
     2461 tgcagtagcc cttcgggccg cggggggcag ccgggaacat acacgtccac ggggaggatc
     2521 tgatcgatgc ccttggtcac agagtaggag tcccaatagg gccctccgca gtttgcgcag
     2581 gaacccatcg agatcacgta cttgggctcg ggcatctgct cgtaaacccg cttgatggcc
     2641 ggagccatct tgtcggtcac cgtacctgca acgaccatga gatcggcttg gcgtggagag
     2701 gcagggaacg ggatcacccc gagccgcatg atgtcgtgct tcggcatcgc ggatgcgatg
     2761 aattcgatcg agcagcaggc gagcccgaac tggaacatcc acaaggaata cttgcgactc
     2821 cagttcagca ggaaggagac cggcttcggg agcttgatct tccccaccgg tttgggctca
     2881 gacactcaga cccactccag taccttcttc cgccaggcat agaccaaccc cagcgccagg
     2941 atgccgatga agatcatcat ctccacgaac gcgaagatgc cgatcttctc gaaaacggtc
     3001 gcccacggaa acaggaacac cgcttcgacg tcgaagatca cgaagagcag ggcgaagatg
     3061 tagtaccgaa cggtcgactg cgtccaccca ccgcctatcg ggtcaactcc gcactcgtag
     3121 gtcatgtact tctcctcagt acgtaccctc ggagcgatca accgattgag aagtagggtc
     3181 cccccaacga gcacgaagac ggcccccgcg aatatcgcga gagtcagata ggaaccgtag
     3241 taacccgtgg gcatatgaac gaactctccg tcgagtggct tgctggcacg tgaaaattat
     3301 cacagtgacc gttgccctca gcggcgggtc cctcgggccc gtagaaccgc caccggccgc
     3361 gacccagcgg cggatgaggt tcgggggccg gtgagtcgcc ctcgggaccc gccgcctcga
     3421 ggatttgtcg cggtcttttc gcgaataggt acctcagagg tagctggtta ccataagacc
     3481 ggcggcgcgc cttcttgtgg tcagtggccg gaggtgggcg gttcgccggg ggtgcaggcg
     3541 gcccagggca tgtgggtcgt gttgtggatc gcgaagaatg atccggttcg gtcgatgacg
     3601 atcaggcccc cgagcccctg cgtgcgcctc tcgaacgtta gcatggcctc cttgctggcg
     3661 atctcgggac cggaagtggc aaccctggcc acaacttccc tcgacaggag cactttcaag
     3721 atcgactcac catgcccggt ggcgctggct gccccgccaa gctcagcatc ggcgtatgta
     3781 ccggcccccg gcatgggcgt atctcctact cggccgaccc tcttatagag gatgcctccg
     3841 gtcgatgtgg ccgccgccag gttcccatct gcgtcgagag caacggcccc gacggtgcca
     3901 gcccccgaga tcccttgacc attcgtgcga cgagcggtct cagctatcaa ttgagcccgc
     3961 acgcggtcga gtatcagatc ctccgggcct agcggcctga tgccgctctc catagcaaag
     4021 ttgagagcgc cctcgccgac gagaagggaa tactccccgt cctcatagac ccgtagcgcc
     4081 agttcgatgg cgctcggtat gggtgggcag gcccccaccg cgccgaatct caggtcgccg
     4141 gtcatgacag cggcgtcgag ttcgaccgat ccctgtcgcg tgagagtggc gccaagaccc
     4201 gcgttgaagt tggggttcgc ctccatcgaa cctacggccg cgaccgccgc cgcgagtgcc
     4261 cccgaaccta gcgactcagc tccggtcttg gccgctgctt cgacaccctc gaggcaagca
     4321 tccagacgcg tgcgatccat cagtcccgcc ccgccatgaa ccacaactcg aggcgttgcc
     4381 atcagtattc gggcttgaag gttcgggtga catcgttgat cgcatcccag agtttcggga
     4441 agaggggttc ggtcgcgccc gcgatcagga cagtggcggg cgcccccttg agcgatctca
     4501 catcgagccc tatctcacga cgggcgagat tgagatggtg atagcgaaac tcctggaagg
     4561 actgatcgaa gtcgatgatc gcggctatca actcgtggag atcaggcttg ccctcgggat
     4621 ccatccacac atcgaatggg gccacacggt gtccatccag gaggggctcg agggttttcg
     4681 agaccatcgg gccgacctcg gtcaggagga aacggaaacc gggtgactcg ctgcccgacc
     4741 cgcgtcccag gcccatgcgg atgcgctgat agttcgcagg ggccatggtt tcgaggatgc
     4801 caagttcacg gctgatcaat cgctcgatca tggcgcagcg tcggaatgat gccgacgctt
     4861 cgcgcagaga accttcctcc atcttcgaga ctgcgtacgg gacctcgtgt tcgatgactt
     4921 tcatccatag ttcggccgtc tggtgagtga cctggaagag acgctcgtcc tgattcacga
     4981 gaaggtcagc aggcttcagc gcagcgaaca actgctctgt gccgacataa ctctcgtatt
     5041 cactgatcct gggcatgatc acctctcaac ttgggttcga cagcttaggc cttagtggaa
     5101 cgcgaatccg gcatgacggg cggcatccag aagctgggtt ggcaggactc cgatcagcag
     5161 gatgaagacg atggagatga tcatcacgat cccgagggat ggatgagtgc tcgccggttc
     5221 gggggcctcg tcatcctcaa gaaacatggc aacgataact cgcagataga agaaggccgc
     5281 gaccgcgctg gtcatcacgg ctatgacaac tacgggggtc aggcccgccg agacaccggc
     5341 agagaacacc tcgaacttcg cccagaatcc cgctgttgga gggatacccg ccaaggagat
     5401 caggtccact gcgagaacgg cggctaggaa cggctgacgt cgccagagcc ccctgtagtc
     5461 ccttagctcg acaagcgact cgccagaacc gccaacggca tagatcactg cgaacgcacc
     5521 gatgatcgtg atggcataga ccgcgaggta gaacgcagac cctgaaaccc cgcgctcatt
     5581 gttggcgatg agccctatca gcacgaaccc cgtgtgcgcg atcgcggagt aggcgagcat
     5641 cctcttgaga ttgcgttggg cgatcgccgc gaagctaccc agcagcatcg aagcaacgct
     5701 gatcacgata aggatgggct gccagtccca gcgaagcgat gggaacacca cgtagaagag
     5761 cctcagtgcc acggcgaatc ctgcgatctt cgagccggcc gccatgaaac ccgctactgg
     5821 cgcgggggcc ccctgatacg cgtcaggggt ccacatgtga aacggcaccg ctgagatctt
     5881 gaagaccaac ccgatcgcga gcagggctat gccgggaacg aggagtccct gttggaggat
     5941 cgagacctct cgtacgctac gcgcgatgcc caagagacgc gtcgttccgg tggctccata
     6001 gaccaacgca atcccgtata ggaagaaggc actcgagaag gcacccgtca ggaagtactt
     6061 gagagaaccc tcctgcccat ccgccctgcc acgtcgcagc gcaacgagga tgtagagcgc
     6121 cagcgagaag gtctcgaggg cgatgaagat gacgatgagg tccgttgccg ccgtcatcag
     6181 caccatcccg gcggtcgcga agaggacgag gccgagagct tcaccgatgg ggcccccctc
     6241 cgacctcaga tagccatgca tcgcaaggat cgtaagcgca gccgttgcga gcagagccat
     6301 cttgaggaag atcgcgaacc catcgacagc aaccgtgttg tcgaaggctc tcgaaacgcg
     6361 cccgatgctt gggaccaaga acccagcggc tgtgagaacg atcgctagag ccgcgagggc
     6421 aagggggccc ggccgagccc tgcgtacgaa agcacccccc agaagcagga gtagacctcc
     6481 taccgcaagg accccctcgg cggcgatggc ccaaagatcc acaccctgga tgtgaaggtc
     6541 cggcactact tgcccgccaa tctcagcgtg ggctgatgga cgttatcgat gagccgagcc
     6601 acggacggtt cgatgcgttc gaggaagggc ctggggaaga tgccgatcac gaacgacatg
     6661 accacaagtg gagcaacgac caatacttca cgcagtgaga catccgacca gatgctcttt
     6721 ctctcctggt gaggaagatt cgctggtccg gtgaagaccc gttcgtatgc ccacaacaga
     6781 tatatcgccg cgaggaccac ccctgatgtg gcgatcacgg cgatgggccg cgagtaagtg
     6841 aaggatccat tcaggatgag gaactccccc acgaacccat tcagccccgg tagacccaac
     6901 gaagccaggc tcgtgatgag gaatagaccc ccgaataccg gcatcaaacg gttcacgcca
     6961 ccgagttccg atatctgtcg agtatgtgcg cgctggtaga gcatcccgat gagaaggaac
     7021 agcgcgcccg tcgtcagacc atggttgatc atctgcagga cactgcctga ggttgcgatc
     7081 tgattgagga ctccgatacc cagaaccacg aaaccgaggt gggagacgct cgagtacgcg
     7141 accagacgct tgatgtcgcg ctgaacgatg gatactaggc ccccgtaaac gatgccgatg
     7201 acacccagca cgaccatcac cggcgcgaat cgcgccacgg cctcggggaa taagggaaac
     7261 gagaacctca ggaacccgta cgcacccatc ttcagaagaa cgccggcgag caacacgctt
     7321 cctgcggttg gggcttcggt atgtgcatcg gggagccatg tgtggaacgg gaagatcggg
     7381 atcttgatcg cgaatgaagc gaagaaggcg gcgaatagcc agagctgtgc cgagtgtgcg
     7441 aagtgaacct gggcaagctt ggtcaggtcg aatgtatggg ggccgaggaa gtagagagag
     7501 acgatcccaa cgagcatcaa cagcgaaccg gtgagggtgt agagaaagaa cttcactgcc
     7561 gcgtagacgc ggcgctgata accccagatc ccgatcaaca gatacatcgg gatgagcatc
     7621 ccctcccaga agacatagaa aaggagaagg tcgagcgcga gaaagacgcc gaggatcccg
     7681 gtctcgagca cgagcagaac cagatggaat gacttgacgc gatcggtcac cttccatgag
     7741 gccaggacac acagaggcat gagaagcgtg gtcaacagaa ccaaccagag acttatgccg
     7801 tcgatcccca ggtgatagcg
//
LOCUS       DATDOZ010000136         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3783707, whole genome shotgun sequence.
ACCESSION   DATDOZ010000136 DATDOZ010000000
VERSION     DATDOZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3783707"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VI541_02635"
     CDS             <1..1146
                     /locus_tag="VI541_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HLF69833.1"
                     /translation="VVQAGEVVADVPAGSLGDGPLYDRPSHKPEWLEAIGAIDVLVPP
                     TDLGQALLAILGSPNIASKRWVYEQYDHMVMLGTVVKPGSDAAVLRIEGARSRIALTV
                     DGNGRYCHIDPRAGSAHSVAEAARNLSALGARPVAITNCLNFGNPQRPEVMWQFKEAI
                     AGIAQACDALGTPVTGGNVSFYNESADLAIYPTPIIGMVGILDHDVTPPVTGFAEGRI
                     VAVVGDTLPELGGSEYARSVLGLVGGKLPTLDLAREATTGETVRSAIASGLLASVHDI
                     SSGGIGVALAECAVLGSTGVLVTPPDDLDAYLWLFSESASRYLVSFDEVHVERLRGDF
                     AEAGCGLHVIGRTVGDRLIVEGALDLGVPELRSAYMDSFARAMSDGG"
     gene            1221..2639
                     /gene="purF"
                     /locus_tag="VI541_02640"
     CDS             1221..2639
                     /gene="purF"
                     /locus_tag="VI541_02640"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HLF69834.1"
                     /translation="MGLGSDQPKEACGVAGVYAPGEQVSKLIYFMLYALQHRGQESAG
                     IAVSEGDVLVVVKEMGLVAQVFDENTLSSLTGGVGIGHVRYSTTGSSLWDNAQPIYRA
                     YGGLTVALGHNGNLVNTLDIARDLEAFGAANRTATTDSDLIAELIVRSGKETVEQAIL
                     DTLPKLKGAFTLVMMDEKKIFGARDPHGLRPLVIGRLASGGWVLASETCALDNIGATV
                     VREVEPGELVVIDEDGIHSHRYAEATPKLCIFEFVYFARPDSNLYGRNVQMTRFRMGQ
                     RLAQEAPADADLVMPVPDSGRGAAAGFAQASGIPYGDGLVKNAYVGRTFIEPTQMIRE
                     QGIRTKLNPMAEMIEGKRLVVVDDSIVRGNTTRQIVKMLRDAGALEVHMRISSPPIKW
                     PCFYGIDTANRDELIAAWKSVDEIARHIGADSLSYLSTEGMVAATEVSADMFCTACFS
                     NEYPIPIPDEVKVTKHMLEGIV"
BASE COUNT          514 a          836 c          850 g          533 t
ORIGIN      
        1 gtggtccagg ccggcgaggt ggtcgccgat gtccctgcgg gatcgctggg tgatgggccg
       61 ctatacgacc gcccctcgca caaaccggaa tggctagagg ctatcggcgc gatcgacgtt
      121 ctcgtgccgc cgaccgatct cggacaagcg ctgctcgcga tactcgggtc gcccaacatc
      181 gcctcgaaga ggtgggtata cgagcagtac gaccatatgg tgatgctggg caccgtcgtg
      241 aagcccggat ccgatgcagc ggtcctgcgg atagaaggag cccgctctcg catcgcactc
      301 acggtcgatg gcaacggtcg ctactgccac atcgacccac gagctggttc ggcacactcg
      361 gtggctgagg ctgctcgcaa cctctcggcc ttgggggccc gacccgtggc gatcaccaac
      421 tgtctgaact tcgggaaccc ccagcgccca gaggtgatgt ggcagttcaa ggaagccatc
      481 gctggcatcg cgcaggcctg tgacgctctc ggcaccccgg tcacaggtgg caacgtcagt
      541 ttctacaacg agtctgcaga tctagccatc taccccacgc cgatcatcgg gatggtgggg
      601 atcctggatc acgatgtgac ccccccggtc accggtttcg ccgagggtag gatcgttgcg
      661 gtcgtggggg ataccctccc cgaactcggg gggagtgagt atgcgaggag cgtgcttggt
      721 ctcgtcgggg ggaaacttcc gaccctcgac ctcgcccggg aagcgaccac cggcgagacc
      781 gtaagatcgg cgatcgcttc gggtctcctt gcatcggtcc acgacatctc atcgggcggc
      841 atcggagtgg ctctggccga gtgtgctgtc ctgggatcga ccggagtgct ggtcacaccc
      901 ccggacgatc tcgacgccta tctctggcta ttctcggagt cggcatcccg ctacctggtc
      961 tcattcgatg aggtgcacgt cgaacgtctc cggggcgact tcgccgaggc cgggtgcggc
     1021 cttcacgtga tcggccgaac cgtaggcgac aggctgatcg tcgagggagc cctcgacctg
     1081 ggtgtccctg aacttcgaag tgcctacatg gactcattcg cgagggcgat gagcgacggt
     1141 ggctagatcg gtgggctcgc ccaactgcgt ctggacgctt ccttcatcgg ttaacctagt
     1201 cttcaaggcg aggggatggt atgggtctcg gttctgatca gccgaaggaa gcctgcggtg
     1261 tagctggggt ctatgccccc ggcgagcaag tgtccaagct gatctacttc atgctctatg
     1321 cattgcagca ccgcggccag gagtcggcag gcatcgctgt atccgagggc gatgttcttg
     1381 ttgtcgtcaa ggagatgggt ctggtcgccc aggtcttcga tgaaaacaca ctcagctcac
     1441 tcacgggcgg cgtgggtatc ggtcatgttc gttactcaac aactggttcg agcctctggg
     1501 acaacgcaca gcccatctac cgcgcatatg ggggcctcac ggttgcgctg ggtcacaacg
     1561 gcaatctcgt caacacactc gacatcgccc gcgatctcga ggctttcggc gctgcgaacc
     1621 gaacagccac gaccgattcg gatctcatcg ccgagctcat agtgaggagc ggcaaggaga
     1681 ccgttgaaca ggcgatcctc gacactctcc cgaagctcaa gggcgccttc actctggtga
     1741 tgatggatga gaagaagatc ttcggcgccc gagacccaca cggattgcgc cctctcgtga
     1801 tcgggcgtct tgcgagcgga ggctgggtcc ttgcttctga gacctgtgcg ctcgacaaca
     1861 tcggcgccac tgtggttcga gaggtcgaac ccggcgaact cgtcgtcatc gatgaggatg
     1921 ggatccactc ccaccgatac gccgaggcaa cgcccaagct ctgcatcttc gaattcgtct
     1981 acttcgcccg tcccgactcg aacctctatg gacgcaacgt tcagatgacg cgattccgta
     2041 tgggacagcg tctcgcacaa gaggcgccgg ccgacgcaga ccttgtcatg cccgttcccg
     2101 atagtggccg cggtgcagca gctggtttcg cacaggcctc gggcatccct tacggcgacg
     2161 gcctcgtcaa gaatgcctat gtgggccgca cgttcatcga gccgactcag atgatccgcg
     2221 agcaggggat caggacgaaa ctcaacccca tggccgagat gatcgagggg aaacgtctcg
     2281 tggttgtgga tgactcgatc gttcggggca acaccacccg ccagatcgtc aagatgcttc
     2341 gagacgcagg cgcactcgag gttcacatgc gcatctcatc cccgccgatc aagtggccct
     2401 gcttctatgg catcgacacg gccaaccggg atgagctgat cgccgcatgg aagtcggtgg
     2461 atgagatcgc caggcatatc ggcgccgact ctcttagcta tctgtctaca gaagggatgg
     2521 tcgcggcgac cgaggtctcg gcggacatgt tctgcaccgc ctgcttctct aacgagtatc
     2581 cgataccgat ccccgacgag gttaaggtta cgaaacacat gcttgaaggg atcgtgtgag
     2641 gttcagtcgc tcgggggccg aggcccaccg ttcaaagaga accacctacg ccgactccgg
     2701 tgtggatatc tccgctggtc gcagagcggt tga
//
LOCUS       DATDOZ010000137         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3273511, whole genome shotgun sequence.
ACCESSION   DATDOZ010000137 DATDOZ010000000
VERSION     DATDOZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273511"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VI541_02645"
     CDS             complement(<1..1955)
                     /locus_tag="VI541_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HLF69835.1"
                     /translation="MVQRLRTPANRRFRLVLVLAIMFGVTSISTIVALYTDQLWFREV
                     GFQSVFYGIFGTKVSLGLIFGAAFFAVMLVNLLIASRLMPAYGPVLDPRDPFERVRVQ
                     ILPYIRWASLGISAFLAFLFAVGVGQQWEKFLLLRNAVKFGSTDPLFHRDLGFYMFKF
                     PAYSFTYGWAFSSLFMIILVTAGAHFLLGGIRPQAPLGSRVSPQVKAHLSVLIGLLAI
                     LRAFGYRLDQFRLLYSRRGDITGATFTDVHAHLPALQLLFIISLIGAILFFVNIRFRG
                     WALPVAGVGLWILTSILAGGLYPAFVQKFKVVPAQIQKEEPFLRRTIAATRAAYDLEK
                     IKVREFPADPGISQQVLAENGATVSNIRLFSPQILKTAYRQLQEIRTYYQFNDVDVDR
                     YQIGGQLRQVMLSTRELNLTPLESLSWQNNHLFYTHGYGAVVSPTNETSPEGQPLFLL
                     QDIPSRSNEPSLDIKQGGIYYGEGLSDYSLVNTKQKELDYEEQDRSQLTTYAGDGGVK
                     VGGFMRRLAFAWRFRDVNLLISGLVRKESKIIYIRQVRERLEKAAPFLSFDGDPYPVI
                     VDGRIVWVADAYTTSSMYPYSEAVDFGARTAPSSGIGAPTMPGRRNYIRNSVKVTVDA
                     YSGRIDFYIADATDPIVRAYGGAFPGL"
     gene            complement(1956..>2576)
                     /locus_tag="VI541_02650"
     CDS             complement(1956..>2576)
                     /locus_tag="VI541_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69836.1"
                     /translation="GDASASAGRRIKRLLQEDQNELLDRLRSAPASAADALLPQEEQV
                     ERFHGGLNEILSAAFLAGRTAGGLEGGDPARSVESLIRKQFLTPLRSDVARVIEAGFS
                     AKDSAAAISERANDVFRVWKGVRTDLLGEGLVFAAFHAGLIDALVARGTEKKRWIFSP
                     KEQDCPRGICKVNVDGGATATGSVFASGHNAPPAHGGCSCTLIGEE"
BASE COUNT          568 a          765 c          745 g          498 t
ORIGIN      
        1 atcagccccg ggaacgcgcc gccgtacgcg cgcacgatcg gatccgtggc gtcggcgatg
       61 tagaagtcga tgcgcccgct gtaggcgtcg accgtcacct tcacggaatt gcggatgtag
      121 ttgcgccgac ccggcatcgt cggagctccg atcccactag atggggctgt acgggccccg
      181 aagtcgactg cctcggaata tggatacatc gaggatgtcg tgtaagcgtc agctacccac
      241 acgatgcgtc catccacgat caccgggtag ggatcaccat cgaacgaaag gaatggtgcg
      301 gccttctcga gccgctcgcg cacctgccgg atgtagatga tcttgctctc tttccgcacg
      361 agaccgctga tcagcaggtt cacgtcgcgg aatcgccatg cgaaagcgag ccttctcatg
      421 aagccaccga ccttgacacc gccgtcgccc gcgtacgtcg tcaactgact gcgatcttgc
      481 tcctcgtagt ccaactcctt ctgcttcgtg ttgactaacg agtagtccga taacccctca
      541 ccgtagtaga tgccaccctg ttttatgtcg agactgggct cattcgacct cgaagggatg
      601 tcttgcagaa ggaagagcgg ctggccctcg ggcgaggtct cgttcgtcgg actcacgaca
      661 gctccgtacc cgtgggtata gaagagatgg ttgttctgcc aactcaagct ctccagaggg
      721 gtgaggttca attcgcgagt tgagagcatc acctgacgaa gttgcccacc gatctggtat
      781 cgatcgacat cgacgtcgtt gaactggtag taagtccgga tctcctggag ctggcggtac
      841 gccgtcttca atatctgcgg agaaaagagt ctgatgttgg agacggtcgc cccgttctcc
      901 gccaagacct gttgcgagat cccggggtcc gccgggaact ctctgacctt gatcttctcg
      961 aggtcgtaag cagctcgtgt ggccgcgatc gttcggcgca ggaagggctc ttccttttgg
     1021 atctgggcgg gcacaacctt gaacttctga acgaacgccg ggtagagccc ccccgcgagg
     1081 atagatgtga ggatccacag accaacgccc gccacgggca gtgcccaccc cctgaaccgg
     1141 atgttcacga agaagaggat cgccccgatc aacgagatga tgaagaggag ttgcagggcg
     1201 gggaggtgcg catgcacatc ggtgaaggtt gcacctgtga tgtcgcccct tctcgagtag
     1261 agcagacgga actggtcgag tcgatagccg aatgctcgaa gtatcgccag gagaccgatc
     1321 agtactgaga gatgcgcctt gacctgtgga gagactcgag aacccaaggg cgcctgcggc
     1381 ctgatgccgc cgagcaggaa gtgcgctccg gccgttacaa ggatgatcat gaatagactc
     1441 gagaaagccc agccatatgt gaaggagtag gccgggaact tgaacatgta gaagccgaga
     1501 tcccggtgga atagaggatc tgtcgacccg aacttcaccg cgttgcgcaa caagagaaac
     1561 ttctcccact gttggccgac acccaccgcg aagaggaagg ccaggaaggc actgattcct
     1621 agcgaggccc accggatgta ggggaggatc tgcactcgga ccctctcgaa cggatcacgc
     1681 ggatcgagaa ccggaccata cgcgggcatc aatctcgagg cgatcagcag attcacgagc
     1741 atcaccgcga agaatgcagc gccaaatatg agccccagcg atactttcgt cccaaagatt
     1801 ccgtagaaga cggactggaa tccgacctcc ctgaaccaaa gttggtctgt atacagagca
     1861 acgatcgtag agatcgatgt caccccgaac atgatcgcga gaacgagtac cagccggaaa
     1921 cgtctgttcg caggagttct cagtcgttga accatctatt cctctccgat cagtgtgcat
     1981 gaacaaccgc cgtgagcagg cggagcgttg tggccacttg cgaaaactga acctgttgcc
     2041 gtggctcccc catcgacatt gaccttacag ataccgcgag ggcagtcctg ctctttcggc
     2101 gagaagatcc accgcttctt ctctgttccc ctagccacga gagcatcgat gagtccggcg
     2161 tgaaaggcgg cgaaaaccag gccctcgccg agcagatcag tacggacacc cttccatact
     2221 cgaaagacgt catttgctct ctcagagatg gcggctgctg aatccttcgc gctgaacccc
     2281 gcttcgatca cgcgggcaac atccgaacga agaggcgtta gaaactgttt tcgtatgagc
     2341 gactcaacag atcgggctgg gtccccgcct tcgaggcccc cggccgttct tccagccaag
     2401 aacgccgcgc tcagtatctc gttcaggccc ccgtggaacc tctcgacctg ctcctcctgc
     2461 ggaagcagcg cgtcggccgc gcttgcgggg gccgaacgga gtcgatcaag gagttcattc
     2521 tgatcctcct gaagaagtcg cttgatcctg cgtccggctg acgccgaagc atcgcc
//
LOCUS       DATDOZ010000138         4836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1942931, whole genome shotgun sequence.
ACCESSION   DATDOZ010000138 DATDOZ010000000
VERSION     DATDOZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942931"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..136
                     /locus_tag="VI541_02655"
     CDS             2..136
                     /locus_tag="VI541_02655"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HLF69837.1"
                     /translation="MDPLFSLCMARDEGLIDDGDVILLLAAGTGYTWAASVVRWGSAV
                     "
     gene            145..1029
                     /locus_tag="VI541_02660"
     CDS             145..1029
                     /locus_tag="VI541_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF69838.1"
                     /translation="MTGASGFLGGALIAQLRRRGDETVGFDRVPGESVVAGDLWNWAH
                     VMELVAVHRPDVIFHCGALLSAFAEADVAGAIAGNANGTFHVLEAARHFGARVVFTST
                     IATYGRGIGEIVGDDSPQFPSSIYGVTKVFGERLGEYYNSRYGIDFRAIRFPSVVGPG
                     RGDSGLSAYSSLMIERPLAGEPYVVPLEPSTCIPILYVRDAVRALIELGEAADERLTR
                     RGYLLAGISPSAGDIAIEVARRAGTGVISFNADATAQAIVDSWPRAIDESDAGTDWDW
                     KSEFDLAAIVDAFAAARS"
     gene            1029..1814
                     /locus_tag="VI541_02665"
     CDS             1029..1814
                     /locus_tag="VI541_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF69839.1"
                     /translation="MDGEFEAPGARIAYSVHGEGAPLLLIAGIGYARWCWQWLLPQLS
                     GYRVITMDNRCSGSSSCPQEPFDMSDLSSDCIALLDHLEVERCSVFGVSMGGFVAQLL
                     ALDHPDRVARLILGCTHMGLSKMAMLSDEALAQLSDRSGTPEEQIRRGMPFAFRPGWV
                     DEHPDEFEAIVAKRLEFQPPPEIYMRQLAGTLTFDMGEKAAAIVTPTLVLQGTGDRVV
                     PPDNARLIAETIPGARLEWLDGAGHLFFIEEPARVGGLIDEGP"
     gene            2082..2963
                     /locus_tag="VI541_02670"
     CDS             2082..2963
                     /locus_tag="VI541_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69840.1"
                     /translation="MKSRRWVTAALAVTVAFSLISFLPVAIANHAGTNVMFIRGISNI
                     SNQCEYEWFDNDTATPGIQPVRDILGIPKPKMYANEGPRCRDGNPNLIGIQPVACSLE
                     RNTGTYHAPDQPSNNLLAGETGLEPPCRGDITASDIPGASGCVSLGSAGQSDCGINSS
                     TWYYGYCGQTYGGAKDGIFTYGGQTWLIERMGFTRGRGAWEFSGRMHLTSDVNHKTTF
                     RYYLAAAPDQTSQLLGCDVTHNITSVLFTGTVIVPAPPEPKAYRTEVGWHWCDDDFGP
                     NHSTAAPFKAGTLGENC"
     gene            3063..>4836
                     /locus_tag="VI541_02675"
     CDS             3063..>4836
                     /locus_tag="VI541_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69841.1"
                     /translation="MVTATLLAGTPGVSAPGGMRLVGYFDRRNAKAAVAANGNILIDA
                     AARRAYQAVAVSDTTIFEYDLDTMLVLRSVTIPLLMVADTRAGPVEWLWTLDEVNRRI
                     FTFERRQGSSETYHLMIIDLATLSIGPVVDLWPLGNRLPLAISYDAQSDSVYLMTMVA
                     NINLLPNRGEVYFVEQRTASGRLDWEYRLASCFGAQDQQYPPTLVRSQIQRQFIYLNC
                     YNSGGVQGQIVRLHLRADGQVESGADEVFPAVPPAFSTMFDRGSDRMFFLTTNSGAGR
                     GAWVFDGLRSTFLGVIATGDSKGGFDYSMGLDPVTGRLYLHSPIGLVVADARRTPLGG
                     GLVFHEYAGFGTGAIQVDPATRHVFVPDTTSRNSVGQPLRYMVLADELQASSATVIPD
                     PDALTTDVAEAPNVTGVNFSGAASAFGLRTLTTGGLQRGAWNIALGQFSTDELQVVWT
                     GVQTVPLDEGNREFHAARVRSVSLTNTGADADAVVSEVDEATRTDLNQVETDWPAQSA
                     ECHDAGGAPDDGIGDQGLAVASCNVGTRSVSASAGVPTLAFDSPTVAVSARALQAASS
                     VSHDSAKGLVSHSSAVVRDLSIAGK"
BASE COUNT          884 a         1422 c         1566 g          964 t
ORIGIN      
        1 ggtggacccg ctcttctcgc tctgcatggc acgcgacgag gggttgatcg atgacggcga
       61 tgtgatcctg ctcctcgcag cgggcacggg atatacgtgg gcggctagtg tcgtgcggtg
      121 ggggtcagcg gtttgaaggc gctggtgacc ggcgcgtctg ggtttctcgg cggagcactg
      181 atcgcgcaac tgcgtcggcg aggcgatgag acagtcgggt tcgaccgcgt tccgggtgag
      241 tcagttgtgg cgggggacct ctggaactgg gcgcacgtga tggaactcgt cgccgtgcat
      301 cggcctgacg tgatatttca ctgcggggca ctgctctcgg ccttcgctga ggccgacgtt
      361 gccggcgcca tcgccggcaa cgccaacggg accttccacg tcctcgaagc tgctcgacac
      421 ttcggggccc gcgttgtctt cacgtcgacg atcgcgactt atggaagggg catcggtgaa
      481 atcgttggtg atgactcacc tcagttccca tcttccatat acggggtaac aaaggtcttc
      541 ggtgagcggc tcggtgagta ctacaattct cgctacggga tcgacttccg tgcgatccgt
      601 ttcccctccg tggtcggtcc agggcgtggc gatagtggcc tctcggccta ctcgtcactc
      661 atgatcgagc gcccacttgc aggtgagcca tatgtggtcc ccctcgagcc ttcaacgtgc
      721 atcccgatcc tgtatgtgcg ggacgcagtc agggcactca tcgaactagg cgaggctgca
      781 gatgagcgtc tcactcggcg cggctatctg ttggcgggga tatcgccgtc ggcgggagac
      841 atcgctatcg aggttgcccg ccgcgctggc acaggtgtga tcagcttcaa cgccgacgcg
      901 actgcccaag ccatcgtcga ctcctggccc cgcgcgatcg acgaatcaga tgctgggacg
      961 gactgggatt ggaagtcgga gttcgacttg gcagcgatcg tcgacgcgtt cgcggcggct
     1021 cgatcctgat ggacggcgag ttcgaggccc ccggggcgcg gatcgcttac tcggtccacg
     1081 gtgagggggc cccgctgttg ttgatcgccg ggatcggtta cgcgcgctgg tgttggcagt
     1141 ggctgcttcc gcagctgagt ggataccgcg tgatcaccat ggacaaccgc tgttccggat
     1201 cctccagttg cccgcaggag ccattcgata tgagcgatct atcttcggac tgcatcgcac
     1261 ttctcgatca tctcgaagtc gagcgttgca gcgtgttcgg agtttcgatg ggaggtttcg
     1321 tcgctcaact gcttgcgctt gatcatcccg accgcgtcgc acggctcatc ctcggatgca
     1381 cgcacatggg tctcagcaag atggcgatgc tctcggacga ggcgctcgcg cagctgtcgg
     1441 atcgctctgg aacaccggag gaacagatcc gccgcggcat gccgttcgcg ttccggcccg
     1501 gctgggtcga tgagcaccct gacgagttcg aggcgatagt tgcaaagcgc ctggagtttc
     1561 agcccccgcc tgagatctac atgcggcagc ttgcggggac tctcaccttc gacatgggcg
     1621 agaaggccgc agcgattgtg actccgactc tcgtcctgca gggaaccgga gatcgcgtgg
     1681 tgccgccgga caacgcccgg ttgatagccg agaccatccc tggggcgcgc ctcgaatggc
     1741 tggatggagc ggggcacctc ttcttcatcg aggagccagc gcgagttggg ggcctgatcg
     1801 acgagggccc ctagactcca cgagtggctt gactcggacg tcggccgagg cgaataatcg
     1861 ggaagcgaaa ctgacgggtg agtcaggtat tgggaaatgt agaggtcgag ccgcggtgaa
     1921 gttgcggaat aagcgggaag accgacgttt gggggatcac ttctagggtg tagatagaag
     1981 gaaacgagcc aagactcgtc gaactattcg aggagtggct ctggtggtcg agggccgggg
     2041 aagatagcgc gacgagagcc gcgccgatag aggaggtttc gatgaagagt cggagatggg
     2101 taacagcggc actcgcagtc actgtggcgt tctcactgat ctcgttcctg cctgtggcga
     2161 tcgcgaatca cgctggcact aatgtgatgt tcatccgtgg gatctcgaac atcagcaacc
     2221 agtgtgagta cgagtggttc gacaacgaca ccgccacccc gggcatccag cccgtgcgcg
     2281 atatcctggg gatccccaag ccgaagatgt acgcgaacga gggtccacgc tgtcgcgacg
     2341 gtaaccccaa cttgatcggc atacagccgg tcgcctgctc acttgagcgc aacactggga
     2401 cctaccatgc tccagaccag ccctccaaca acctcctggc cggtgagacc ggactcgagc
     2461 ccccatgtcg aggcgatatc acggcttctg acatccctgg tgccagcgga tgtgtttcct
     2521 tgggcagtgc gggtcagtcc gactgcggga tcaactcgtc gacctggtac tacggctact
     2581 gcggtcagac ctacggcggt gcgaaggatg gcatcttcac atacggaggt cagacctggc
     2641 tcatcgaaag gatggggttc acgcgaggcc ggggggcctg ggagttctct gggcggatgc
     2701 acctcacgag tgatgtgaac cataagacga ccttccggta ctacctggcg gctgcccctg
     2761 atcagacgag ccagttgctc ggttgcgacg tgactcacaa catcacctca gtcctgttca
     2821 ccgggacggt tatcgtccct gcacccccgg aacccaaggc ataccgaacc gaggtcgggt
     2881 ggcactggtg cgatgacgac ttcggcccga accactccac cgccgccccg ttcaaagccg
     2941 gaacactggg cgagaactgc taacggcgca gggtcaacag atctgaagga gaagtgatga
     3001 cgcgaaggag accggagggc aacctccggt ctcttcgttt ccggagattg atttgtgggg
     3061 gtttggtcac ggccactctc ctggcaggca ctcccggtgt atcggcgccg ggggggatga
     3121 gactcgtcgg ctacttcgac cggcgcaacg cgaaggcggc cgtggcagcg aatggcaaca
     3181 tcctcatcga cgcggccgcg cggcgcgctt atcaggccgt agctgtttcc gacacgacca
     3241 tcttcgagta cgacttggac acgatgctgg tcctaaggtc agtcaccatc cctcttctca
     3301 tggtcgccga tacgcgagct gggcccgtgg agtggttgtg gacattggat gaggttaatc
     3361 gccggatctt cactttcgag cgccggcaag gtagctccga gacctatcac ctcatgatca
     3421 tcgatctggc gacgctgagt atcgggccgg tagtggatct ctggccattg ggtaaccgac
     3481 ttcccttggc catctcttat gacgcgcaga gcgacagcgt ttatctgatg accatggtcg
     3541 ccaacatcaa cctgcttccc aatcggggcg aggtctactt cgtcgagcaa cgaaccgcgt
     3601 cggggcggct cgactgggag taccgcttgg cgtcttgctt cggagcgcaa gaccagcagt
     3661 atccgcccac gctcgtcaga agccagatcc aacggcagtt catctatctc aactgctaca
     3721 actccggtgg tgtgcagggt cagatcgtcc gactgcatct gagagccgat gggcaggtgg
     3781 agtcgggagc cgatgaggtc ttccctgcag tccccccagc cttctctacc atgttcgacc
     3841 gaggttcgga ccggatgttc ttcctaacaa ccaacagcgg cgcagggagg ggggcctggg
     3901 tcttcgacgg gctacgctcg acattcctcg gcgtcatcgc gacgggtgat tcaaagggcg
     3961 gcttcgacta ctccatgggc ctcgacccag ttacaggcag gctttacctc cactccccga
     4021 taggtttggt agttgcagat gcgcgccgga cacctcttgg gggaggactg gtgttccacg
     4081 aatacgcggg attcgggacc ggggctatcc aggtggatcc ggcgacgcgc catgtgttcg
     4141 tccccgacac aacctctcgc aacagtgttg ggcagccact tcggtacatg gtcttggccg
     4201 acgaattgca ggcgtcttcg gcgaccgtca ttcccgaccc cgatgcacta actactgatg
     4261 ttgctgaggc tcccaacgtg acgggtgtca acttctcggg ggcggcgagc gcgtttggac
     4321 tacgaaccct taccaccgga ggtctgcagc ggggggcctg gaacatcgcg ctgggtcaat
     4381 tctcaacaga tgaactccag gtggtctgga cgggggtcca gacggttccc ttagacgaag
     4441 gcaacaggga gtttcatgcg gccagggtcc gatcggtctc actaaccaac accggtgccg
     4501 acgcggacgc tgtcgtcagc gaggtcgacg aggcgacgcg caccgacctg aatcaggttg
     4561 aaaccgattg gcctgcacag tcggcagagt gtcacgacgc gggaggtgcc ccggatgacg
     4621 ggatcggaga ccagggtctc gcggtggcct catgcaacgt tggtacacgc agtgtgtcgg
     4681 cgagtgcagg ggttccgacg ctggccttcg acagcccaac ggttgctgtt tctgcccgcg
     4741 ccctgcaagc ggcttcgtcg gtttcgcacg attcggccaa aggcttggtg tcgcactcgt
     4801 ctgcagtcgt gcgagacctg tcgatcgctg ggaagg
//
LOCUS       DATDOZ010000139         5735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3794229, whole genome shotgun sequence.
ACCESSION   DATDOZ010000139 DATDOZ010000000
VERSION     DATDOZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794229"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VI541_02680"
     CDS             complement(<1..339)
                     /locus_tag="VI541_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLF69842.1"
                     /translation="MKAVVYEKYGSATVQFTEVEKPVPTDDQVLIRIRASSVNGPDRE
                     SVFGRVPYGSQPPPGKHIRMGGRRQVSRRILGSDIAGQVEAVGKDNTEFKLGDEVFGE
                     VGGYSGGFADY"
     gene            complement(901..1476)
                     /locus_tag="VI541_02685"
     CDS             complement(901..1476)
                     /locus_tag="VI541_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69843.1"
                     /translation="MSEKTTQGSLDSVAMVTAQRFVRHGSRGVALAAALSFLGAALYA
                     VTWKSESGFHPVAAVPWFAGLAVFGLSIVLGLPALFAGTRDLFRRNRGKVAAKLLLFF
                     GPLVVFVGMELLPHLINPCTITEAIGRPTLPQMCEEMTYRSGFQGEGKLVTDFDIKDR
                     WHPLSHSLFGAIPLAALYGLALRKRKIDADA"
     gene            complement(1592..2116)
                     /locus_tag="VI541_02690"
     CDS             complement(1592..2116)
                     /locus_tag="VI541_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69844.1"
                     /translation="MSRFRGLLFAFVVLAAFAAAPFAGAGGIGSDGKDCGDGSGGHEL
                     TWSPTTVWPPNHKYVDVTITYSDEDGDHDLTLAATSATHDELDLPGAGNTDDDVVLPA
                     DAATGKDSVSVIVRVRGERSGLGDGRTYTVDYSASSTDEDGDEDNDCSGSITITTPHD
                     CREMRRSCGTAARS"
     gene            complement(3015..4922)
                     /locus_tag="VI541_02695"
     CDS             complement(3015..4922)
                     /locus_tag="VI541_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HLF69845.1"
                     /translation="MSQRIFDGLDPEQLSAVRAIRGPVCILAGAGSGKTTTITQRIAN
                     QVASGAFEPGQILAVTFTDKAAKEMASRLATLGAGAVRARTFHAEALAQYRQLSSEEF
                     EILGSKVQVLKSLVQSLPMPHRFTAVRDIATEVEWAKNQLITPVDYLDRLGPHTPPIP
                     PELMATVFERYEKRKASAKLIDFEDLLARAVEVLQEPAAVRQIRDRYLAFTVDEYQDV
                     NLLQQSLLDAWVGDRRDICVVGDDYQSIYSFTGASASHLLGFKQRYPDAKVLLLTSNY
                     RSTPQILDVSNRLVPKMLGSSKLLRPTRGPSQTPAIQGFASAEDEIAHVISSIKRLVT
                     EGIALEEIAILYRINARSEPFEEALSEARIAYQVADSPFLRRPGPRAALQRLRRIGSA
                     DVAASVEDVVHSLGYQADALGADGDEATRQADLGRLVAMAREFSAGGVQEFVADLVAR
                     FTAESDGRGVQLLTYHRAKGKEYEAVFLPRLEDGELPFSLSRTDDQIAEERRLFYVGI
                     TRAKTHLSISWTSWREAERRSRPSPSPFLSELGATPMEYRKPRLQPMKPSVPESDLFL
                     ALKRWRSEVAREKNVPAYIVFQDATLTSIAEAKPTTPRGLLQVKGVGPAKCEEYSAAI
                     IELVQADGTRH"
     gene            complement(5038..5517)
                     /locus_tag="VI541_02700"
     CDS             complement(5038..5517)
                     /locus_tag="VI541_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69846.1"
                     /translation="MRNRGIALVMIALLVALVGCNRVKSTVKAPDKPAKQPASGEGVS
                     STVQLDESAKTKIDGNKISFEHIVGSSPCPQLIATITLANIEGFGTIDFEVSIGRPLS
                     ATPTSGQFTHEEDAVIQIFFDCSQQTSFEKTFAITHNTLDQKSEGKIVYNVSGNVHR"
BASE COUNT         1152 a         1758 c         1713 g         1112 t
ORIGIN      
        1 gtagtctgca aacccgccgc tataccctcc cacttccccg aagacctcgt ctccgagctt
       61 gaattcagta ttgtccttcc cgacggcctc tacttgcccc gcaatatctg atccgagtat
      121 ccgtctgctg acttgtcgac ggcctcccat gcgaatgtgc tttcccggag ggggctggga
      181 accatatggg actctcccga acactgattc tcggtccgga ccgttaaccg aggatgcacg
      241 aatcctgatc agaacttgat cgtcagttgg aacgggcttt tcgacttccg tgaactggac
      301 ggtcgctgaa ccatacttct cgtagacgac ggccttcatc agttcccggg gcatgccgtt
      361 aaccttagcc agaagccctg accagggaaa ccggggttcg tgttatgtgt cgttgcctcc
      421 ccttgatccc gggataacgc gacgcctggc gaactacctc gaaaaacctc gcggcggacg
      481 aagcttgggt tcgactcgga accttgttct gacgtagcga tctccgatga cctgaaatcg
      541 tggaaccgga aagaagagga cgcctagtac gccataaagt cagccaggat cgcgcccgaa
      601 gtggccccac ggatcagcgc cggaggtgag cgaacagccc catcttgccg tccctgaggg
      661 gggcttcccg cctcgctcgc ggcaccagcg agaacccatc cgcgccggga gacaggaaga
      721 acaccacctt cccttagcct ctcatccttc gtccgatcag gtccctcaca gttccgtccc
      781 atgcagtggc catgcccgtt gtcaccctca agccacgctt ccccgtcaaa gggggtattc
      841 gctctgtcgg gtcggcgtcc gcgaagagaa cacgacacga ctggttacgc caagtaagga
      901 ctatgcgtcg gcatcgatct tccgcttacg gagagcaagc ccatagagag ccgctagggg
      961 tatcgctccg aacaggctat gggacaaggg gtgccaacga tccttgatgt caaagtctgt
     1021 tacgagcttc ccttcccctt gaaaacccga acgatacgtc atctcctcac acatctgggg
     1081 cagcgtcggc ctaccgatcg cttcagtgat cgtgcacggg ttgatgaggt gcggaagcag
     1141 ttccatcccg acgaacacca cgagaggccc aaagaacaag agtagctttg ccgcgacctt
     1201 gccgcggttg cgtctgaaca ggtcccgcgt acctgcgaac aaggcgggta gtccaaggac
     1261 gatcgacagt ccgaaaacag ctagccctgc aaaccaaggc acggcggcga caggatggaa
     1321 gccgctctcc gatttccaag tgacagcgta caaggccgcc cccagaaacg agagcgctgc
     1381 agcgagagcc acaccgcgag atccatggcg cacgaatcgc tgagcggtca ccatagccac
     1441 cgagtctaaa ctgccctggg tcgtcttctc ggacatctaa gcacccctta cgagagtatc
     1501 gagggtgccg tcatgttcct tccgcctcgg tctcctcttc gccgcggcaa agttgacagg
     1561 gtaaggggtc tacctggaag tcgctcagtc cttaagaccg agcggcggtt ccgcagctcc
     1621 gccgcatctc acggcagtcg tgcggagtcg tgatggtgat cgagccgctg caatcgttgt
     1681 cttcgtcacc gtcctcgtca gttgaactcg ccgagtaatc gacggtatag gtgcgaccat
     1741 cgccaaggcc cgaccgctcc ccgcgcactc tcacgatcac gctcaccgaa tccttgccgg
     1801 tggctgcgtc ggccggaagc accacgtcgt catccgtgtt gcccgcgcca ggaagatcga
     1861 gttcatcatg agtggcggaa gtggcggcaa gagttagatc gtgatcgccg tcctcgtcac
     1921 tgtaggtgat tgtgacatcg acgtacttgt ggttgggtgg ccaaaccgtc gtgggtgacc
     1981 aggtgagctc gtgtccaccg gatccgtccc cgcagtcctt gccgtcgcta cctatgcccc
     2041 ccgccccggc gaatggtgcc gcggcgaatg cggcgagaac tacaaacgca aaaagcagtc
     2101 cccggaatcg tgacactcgg tacctcccta tgatcgagca atctccctag ccaacgagcg
     2161 atagctgcgg ttcgttacgg tcccgcccgg gtcccaaccc aggacagaga gcatagaagt
     2221 atgacatttg aggaaatgag aggtcaactg caccctttgc gttggattgg ggcgaaggaa
     2281 ggccgaggga agacgagcag ccgatcgcta tgccctcttg gagaccccac gcagtcggtt
     2341 accggcgcgc gggttctggc ctcgcttcgg ctggagccgc tagccgtgtt ttcgacgtgg
     2401 gaaccggacc aggtacgctg cttgcagacc tttccgaagg actccagagg cgatcgtagt
     2461 tggcagtgac gtctcggaag gtgcgatacg cctcgcccgt cgagacatcc cgatccactt
     2521 gcggtgatgg actcgataaa gcttgctttc gcggatcaga ccttcgacgt ggtcatgttt
     2581 tccttctcgc cttttcactt acgcaggccg gcctacgcac gaggttgccg tacggcctct
     2641 tcaacaagcg cccttcaagc atgatcaact tgggtttcgt tagaaccgcc gcatcaagcc
     2701 gaggatccca acggacgtag cgaagggatg accggaccgt cgcgtacttc ggctgctcgg
     2761 atatcgagaa gagccttcag gtcctcgtag aggcgggagg cgagcaagtg gaagcggtca
     2821 gggacgtcgg gggcgagaaa ctgatcgcat cggcgaggga ctcgaacggc aacgtcatcg
     2881 ggctcatcca gtcggaggcg tgaccattag gccgccagct tcggtaggga gattccgaga
     2941 aatggctgat tcatggcgca ttgaggagca ctacctgatg gcctgcaact gtgatatcgg
     3001 ctgcccgtac aacttcaatg gcgggtgcca tccgcttgga ccagttcgat gatcgctgcg
     3061 gaatactcct cacacttcgc agggcccacg cccttcactt gcagaagacc acggggcgtc
     3121 gttggcttcg cctcggcgat agatgtaagt gtggcatcct gaaagacgat atatgcgggg
     3181 acgttcttct cgcgagccac ctcactccgc cagcgcttga gggccaagaa caggtccgac
     3241 tccggcaccg acggtttcat cggctgaagt cggggcttcc gatactccat gggtgtggcc
     3301 cccaactcgg acaggaaggg tgatggtgat ggacggctgc gtctttcagc ctcacgccaa
     3361 ctcgtccagc tgatcgacag atgggtcttg gcccgagtga tgcccacata gaacagcctg
     3421 cgttcctcgg cgatctggtc gtcagtgcgc gatagagaga acggcagctc gccgtcctcg
     3481 agacgcggca ggaaaaccgc ctcgtactcc ttacccttcg cccggtgata cgtgagcagc
     3541 tgaacgccgc ggccatcgct ctcggcggtg aagcgcgcga cgaggtctgc cacgaactcc
     3601 tggactcctc cggctgagaa ctccctggcc atcgcgacga gccttccgag gtccgcctgg
     3661 cgggtcgcct catcgccatc ggcccccaac gcgtcggcct ggtagccgag ggagtgcacg
     3721 acgtcctcga cgctcgcggc gacatcggcg cttccgatac gacgcagcct ctgcagagcc
     3781 gctcgagggc ctggacggcg aaggaacggc gagtcggcaa cctggtaggc gatgcgagcc
     3841 tcggacaagg cctcctcgaa aggttcagac cgcgcgttga tccggtagag gatcgcgatc
     3901 tcttccagcg cgatgccttc ggtgacgagc ctcttgatgc tgctgatgac gtgagcgatc
     3961 tcatcctcgg ctgaagcgaa gccctggatg gcaggagtct gcgagggccc gcgggttggt
     4021 cgaagcagct tcgagctgcc gagcatcttc ggaacgagcc tgttcgagac gtccaggatc
     4081 tgcggcgtcg accgatagtt cgacgtcagc agcaagacct tcgcatcggg atagcgctgc
     4141 ttgaacccga gaagatggct cgcgctcgcg cccgtgaacg agtagatgga ttgatagtca
     4201 tcgcctacga cacagatatc gcgtcgatcg cccacccatg catcgagaag gctctgctgg
     4261 agcaggttca catcctgata ctcgtcaacg gtgaacgcca ggtagcgatc ccggatctgc
     4321 cgaactgccg cgggctcttg gagtacctcc accgcgcggg ccagaaggtc ctcgaagtcg
     4381 atcagcttcg cgctcgcttt acgtttctca tagcgctcga acacagtagc catcagttcg
     4441 ggggggatcg gcggcgtgtg ggggccgagg cgatcgaggt agtccacagg cgtgatcaac
     4501 tggttcttgg cccactcgac ctcggtcgct atgtcgcgca ccgcagtgaa gcggtgcggc
     4561 atcgggagac tctgcaccaa agacttgagc acctgcacct tggagccgag gatctcgaac
     4621 tcctcgctgc tcagctgccg atattgggcg agtgcctcgg cgtggaaagt acgagcacgg
     4681 acggccccgg cccccagggt cgcaaggcga ctcgccatct cctttgctgc cttgtcggtg
     4741 aaggtgaccg ctaggatctg gccgggctcg aacgcgccgc tggcgacctg attggcgatg
     4801 cgttgagtga tagttgtcgt cttgccagag ccggcccccg cgaggatgca aacagggccc
     4861 ctgatggccc gaacggccga cagctgctcg ggatcgagcc catcgaagat acgttgagac
     4921 accagacctc ccagcgcgcg aggagccagc gctccttcct gcctgggatg ataacgacag
     4981 ggtacgacag aaccaggcgg tcctatctga tgactgcccc tttggtcggc tagagcgtca
     5041 tcggtgaacg ttgccagaga cgttgtagac gatcttgccc tcagacttct ggtccagggt
     5101 gttgtgcgtt atcgcaaacg tcttctcgaa tgaggtctgc tgggagcagt cgaagaagat
     5161 ctggatcacc gcatcttcct catgggtaaa ctggccgctg gtaggggtcg cacttagtgg
     5221 gcgcccgatg cttacttcga aatcgatggt cccgaaaccc tctatattcg ccagagtgat
     5281 cgtagcgatc agttggggac acgggctgga gccaacgatg tgttcgaagg agatcttgtt
     5341 accgtcgatc ttggtcttgg cagactcatc aagttgaaca gtcgaggaca cgccctcacc
     5401 cgaagctggc tgtttggctg gcttgtcagg agcctttacc gttgacttga ctctgttgca
     5461 accaaccaga gcgaccagca gcgcgatcat caccagcgcg ataccccgat tccgcatgtc
     5521 ttcctcctca ccccgcggtc gatccgagaa cactacatga gagaaacgac tcagcgccgg
     5581 gcatccacga ggagcgcgag gatagcgaga tgaagatgag ttctgagccg aacccacagg
     5641 cccccgcatc cgcgaccagc ggcatcgccg caggagcggg gatgggagcg gtccacatcg
     5701 ccgtcaccga cggggtacgg gcccttgatt tctat
//
LOCUS       DATDOZ010000140         4135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_898453, whole genome shotgun sequence.
ACCESSION   DATDOZ010000140 DATDOZ010000000
VERSION     DATDOZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898453"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VI541_02705"
     CDS             <1..680
                     /locus_tag="VI541_02705"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLF69847.1"
                     /translation="LMLLGASWPIHDVAEGYLFSVHMVQHILFSLVATPLLILGTPSW
                     MARWLLPAPLMRVFRRIARPLPALAIFNVYLVFSHWPGFVNYVAVNEGAHFLAHFVLV
                     LTAIVMWWPVLSPLSEAPRVSPPAQMLYLFGQTIVPTVPASFLTFAKAPFYSVYANAP
                     RLVASISPTTDQLISGLIMKLGGGLLLWSVIAVLFFRWSSREESEMPDASDWQDLEGQ
                     VNRTGIR"
     gene            677..1282
                     /locus_tag="VI541_02710"
     CDS             677..1282
                     /locus_tag="VI541_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HLF69848.1"
                     /translation="MNPEIRDRFLLPVAIPVGVVIVIATLALLMSSVLLNVPREVAVA
                     VAMMTAFNILAGASVLALRDRVTALHAIPMVAVALLPLIIGGAIAAGVVPLKETEGPE
                     GTEVPSVALIEISADNLLFDKTQLDLPADKPFKLRFDNREDQLHNVQILKEKAGETLF
                     IQPFFKGPSKVTWDVSPIPAGTYYFQCQIHPTTMFGKVVVT"
     gene            1295..3301
                     /locus_tag="VI541_02715"
     CDS             1295..3301
                     /locus_tag="VI541_02715"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HLF69849.1"
                     /translation="MKRFQRLAAVASGLTLAQVAVGALVRATRSGLGCGNDWPRCHGR
                     LLPAMADRAVAIEFSHRLVASIVIFAVSALAIYAWLKYRNVRSIFWPSFAAMVLVFGQ
                     GAIGGLVVLADLARLSVVLHFIVASTLLGVLTYVTVRSGSMRQGGWHPPAKRALFAMC
                     GVFVALLIGAFVSQTPGAPLVFPDWPLMNGTIGSPAGTVARLHWVHRLVAVLLGVALI
                     DLAVRATRRSPRDRHEIRYAHGLVGLWAIQVGLGALNVFTRSASWAIVLHVIAASLLW
                     MLSVALVVHLYRRDGAKESSTESVASDEPGLGDRVKAYFLLTKPRIIELLLITTVPPM
                     VVAAEGWPRTSLVLATLFGGMLTAGAANSINCYYDRDIDERMERTASRPLPAHRVTPR
                     SALRFGVALGVIGGVWMYTLVGPLPAALAVGAILFYVFVYTAWMKRSTPSNIVIGGAA
                     GAVPVLIGWAAVTGHVSAPSWVMFAIIFYWTPPHFWALALRYSDEYAAAGVPMLPVVR
                     GVAETTRQMIMYSAVLMAASLALYPIGAMGIPYLVSALVLGAGFIVHAIRLSRSPSPL
                     AAMGFFRSSISYLVLIFAAMTVDTFIGRPRLPALDGPVLFVSVILFLIFEGLILGAVV
                     GYRRSSPSLRSRTVITEIVWTVTPVILLAVGLLIAWQTGTLAAP"
     gene            complement(3310..4032)
                     /locus_tag="VI541_02720"
     CDS             complement(3310..4032)
                     /locus_tag="VI541_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF69850.1"
                     /translation="MKALMAQARVELTLTIRRGESLLVTIFIPASLLIFFSLVPMIPA
                     ASGTDAVNALLPGVLATAIMASAMVSLGIATGFERQYLVLKRLGGTPLGRPRLIAAKM
                     ISVLAVQVIQTIALIAIAYLLGWTPGKGAWFVVPVIAVGTIAFAGIGLAMAGSLKAEA
                     NLALVNALFIVVLLIGGVLVPLDHLPQVLGATMRFLPASSLAELLRWPLMGGGFPSSA
                     AAITAVWAVVTPTVASRTFRWE"
BASE COUNT          700 a         1269 c         1254 g          912 t
ORIGIN      
        1 tgctgatgct actgggtgcc agttggccga tccatgacgt agcggaggga tacctcttca
       61 gcgtgcacat ggttcagcac atactcttct ctctagttgc aactcccctg ctgatcttgg
      121 gcacaccatc atggatggca cggtggctct tgccagcacc gctgatgcgt gtattcaggc
      181 gaatagcgcg acctctgcca gccctcgcca tcttcaacgt ctatctcgtg ttcagtcact
      241 ggccgggatt cgtcaactac gtcgcggtca acgagggcgc gcacttcctg gcgcacttcg
      301 ttctggttct cacggcgatc gtcatgtggt ggcctgtgtt gagtccgtta tctgaggctc
      361 cccgagtctc gccaccggct cagatgctct atctgttcgg tcagaccatt gttccgaccg
      421 tacctgcctc gttcttgaca ttcgccaagg cccccttcta cagcgtctat gcgaacgccc
      481 cccgattggt cgcgagcatc agcccaacca ccgatcaatt gatctcgggt ttgatcatga
      541 agttgggcgg cggtctgctg ctttggtcgg tgatcgcggt gctattcttc cggtggtctt
      601 cccgagaaga gtccgagatg cctgacgcct cggattggca ggacctagaa ggccaagtca
      661 accgaacagg aatcagatga accccgagat ccgcgaccga ttcctactgc cggtagccat
      721 accggttgga gttgtgatcg ttatcgcgac cctggcactt ctcatgtcga gcgtgctgct
      781 gaacgttccg cgtgaggttg cggtcgccgt tgcgatgatg accgcattca acatcctcgc
      841 gggggcatca gtccttgctc tgcgagacag ggtcactgca ctccatgcca tacccatggt
      901 cgcggtcgct ctgctccctc tgatcatcgg gggcgctatt gcggccggag tggttccgct
      961 caaggagaca gaaggccccg aagggacgga ggttccatcg gttgctctga tcgagatctc
     1021 ggccgataac ctgcttttcg acaagacaca gctcgacctc cccgcggaca aaccgttcaa
     1081 gttgcgtttc gataacaggg aggatcaact ccataacgtg cagatcctca aggagaaggc
     1141 gggtgagacg ctcttcatcc agccgttctt caagggcccc agtaaggtca catgggatgt
     1201 ctcaccgata cctgccggga cctactactt ccagtgccag atccacccca ccacgatgtt
     1261 cgggaaggtc gtagtcacct aagggtggtc tgatgtgaaa cgtttccaga gactcgccgc
     1321 agtcgcatcc gggttgaccc tcgcgcaggt ggcggtcggg gccctcgttc gcgctacccg
     1381 ctcagggctc ggctgtggga atgactggcc acgctgtcat ggccgcctgc ttcccgctat
     1441 ggctgatcgg gcggttgcga tcgagttctc gcatcggctc gttgcttcta tcgtgatatt
     1501 cgcggtgagc gcgctagcga tctacgcctg gctgaagtat cggaatgtcc gctcgatctt
     1561 ctggccgtcc ttcgctgcga tggtcctcgt cttcgggcag ggcgcgatcg ggggcctggt
     1621 ggtgctggcc gatctcgctc gactctcggt tgtccttcac ttcatcgtgg cctcgaccct
     1681 gttgggggtc ctcacctatg tgacggtcag atcgggctct atgcgtcaag gtgggtggca
     1741 tcctccagcc aagcgagctc tgttcgcgat gtgcggtgtt ttcgttgcgc ttctcatagg
     1801 ggcgttcgtg tcgcaaactc cgggggcccc tctcgtcttt cccgactggc ctttgatgaa
     1861 tggcacgatc ggatccccgg cgggtaccgt cgcccggctg cactgggtac atcggctcgt
     1921 ggctgttctg ctcggagtcg ctctcatcga tctcgcggtg agggccacgc gtcgatcacc
     1981 gcgagatcga catgagatcc ggtacgcgca cgggctggtg gggctttggg ctatccaggt
     2041 cggtctaggt gctctcaacg tcttcactcg ttccgccagt tgggccatag tcctgcacgt
     2101 catcgcagca agccttctgt ggatgctctc cgttgcgctc gtggtccatc tctaccgtcg
     2161 cgatggtgcg aaggaaagtt cgaccgagtc ggtcgcatct gatgagccgg gtttgggcga
     2221 cagggtgaag gcgtacttcc tgctcacgaa gccacggatc atcgaacttc tgctcatcac
     2281 caccgtgccg ccgatggtgg tggcagcgga ggggtggccg cggacttcct tggtcctggc
     2341 gacactcttc ggagggatgc tcaccgcggg cgcggccaac tcgatcaact gttactacga
     2401 cagggatatc gatgaacgga tggagagaac ggcgtcccgc cccctcccag cgcatcgcgt
     2461 gacgccccgg agtgccctcc ggttcggggt agctctggga gtgatcggcg gcgtctggat
     2521 gtacacgctc gtcggcccgc tcccggctgc cctggccgtt ggggccatcc tcttctatgt
     2581 cttcgtctat acggcatgga tgaaacggtc gaccccctcc aacatagtca tagggggagc
     2641 ggccggtgcc gtgcccgtcc tcatcggctg ggccgcggtt acgggacatg tttcggcccc
     2701 gtcatgggtg atgttcgcca tcatcttcta ctggacaccg ccacacttct gggcccttgc
     2761 gctcaggtat tcagacgagt acgcagccgc aggggtgccg atgctcccgg tcgtgcgggg
     2821 agtggccgag acaacgcgtc agatgatcat gtactcagcg gttctcatgg ccgcgagcct
     2881 tgcgctctat ccgatcgggg ccatgggtat cccttatctc gtctctgccc tggtactggg
     2941 ggccgggttc atcgtgcatg cgatccgtct gtcgcgctcg cccagccctt tggcggcgat
     3001 gggattcttc cgctcctcca tctcctactt ggtactgata ttcgcggcga tgacggtcga
     3061 caccttcatc ggcagaccca gactcccggc gctcgacgga cctgtcctgt tcgtgagtgt
     3121 gatcctgttc ctaattttcg aggggctgat cctcggcgcg gttgtcggct acagaagatc
     3181 ctcgccatct cttcggtcga ggaccgtgat caccgagatc gtctggaccg tgaccccggt
     3241 catactgctc gctgtcgggc tgttgatcgc ctggcaaacg gggactctag ctgccccgta
     3301 gcgtcctggt tactcccatc tgaaagtgcg tgacgcgacg gtcggcgtca cgaccgccca
     3361 cacggctgtg atcgcggcgg ctgatgaagg aaaccctcca cccatcaggg gccaccggag
     3421 cagttccgct agggaggaag ctggcaggaa gcgcatcgtc gccccgagga cctgaggaag
     3481 atggtcaagg ggtaccagca cgcctcctat cagaaggacc actatgaaca gtgcattgac
     3541 gagcgccaga ttcgcctcag ccttcaacga tcccgccatg gcgagtccga tccctgcgaa
     3601 tgcgatcgtt cccacggcga tgacgggtac aacgaaccaa gcgcccttcc cgggtgtcca
     3661 tccgagaaga taggcgatgg cgatcaaagc gatggtctgg atgacctgga ccgcaaggac
     3721 cgagatcatc ttcgcagcga tcaagcgggg tctgcccagt ggcgtacccc cgagccgttt
     3781 gagaacgaga tactggcgct cgaaacccgt tgcgatcccc aggctgacca tcgcagaggc
     3841 catgatcgcc gtggccagga ctccgggaag gagggcgttc actgcatcgg ttcccgacgc
     3901 cgccgggatc atcggaacga gcgagaagaa gatcaatagc gacgcgggga tgaagatggt
     3961 aaccagcaga ctctcgccgc gtctgatcgt cagcgtgagc tccacgcgcg cctgtgccat
     4021 gagtgccttc atgggtcgga ggttgtcgcc tcgggagtcg tgagttccaa gaagagcgcc
     4081 tcgagcgaac ccttctgcgt tgatagctcg gttagcaccg tgccttgtga agcca
//
LOCUS       DATDOZ010000141         3843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_116827, whole genome shotgun sequence.
ACCESSION   DATDOZ010000141 DATDOZ010000000
VERSION     DATDOZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116827"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VI541_02725"
     CDS             complement(<1..800)
                     /locus_tag="VI541_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLF69851.1"
                     /translation="MRISERDRNAVVAGLIFATVGVGFYLDSVGAWQIQLVYFWPLTL
                     IAVGISQILGKADRGRRERDRSAKLIVAEERVRIARELHDIVAHSVSLMTVQIAAARR
                     VFDKKPQEAQAALAAAEETGRQSLSELRSIIGVLRSADASIEAARPKPEETQGAWPSF
                     VSSTAEVAASEAEESVATEPLPGIDQIGSLIDTLRTAGMDVTLHVTGPRPRTTPGVEL
                     ALYRVIQEALTNVMRHAGEATVAVRLTSNDSNIDVEVIDNGEGSSGGTP"
     gene            complement(787..1449)
                     /gene="liaF"
                     /locus_tag="VI541_02730"
     CDS             complement(787..1449)
                     /gene="liaF"
                     /locus_tag="VI541_02730"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HLF69852.1"
                     /translation="MTTKATATRPAASRPRTGQVIGVMLLLGGVAWLLEQTGTLSVTT
                     SQVLAAELVVLGLGLIAVSNTRHHGGLIALGLVLILLLANLSSFERRTTEQHLGDLHL
                     NTVVGDLDIAPRSVRSIGAGYRVGVGDLRLDLTHLEIPEGTTEVLVMVGVGDIRVVVP
                     GGSDVSVSASAVVGEVTIFGRKQSFGRVEKTTPGFSDAPKRIKLEIGVVLGDVRVQHA
                     DL"
     gene            complement(1535..2308)
                     /locus_tag="VI541_02735"
     CDS             complement(1535..2308)
                     /locus_tag="VI541_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF69853.1"
                     /translation="MSLVSFQVDERGVARLALNRPDVRNAFNAELIEQITSTIENLPA
                     NVRVLVLSGEGKVFSAGADLDWMRSMAAYSLEENAADSSRLSRMFEALDNCRAATIAR
                     VHGAAIAGATGLVACSDVAIASAETVFAFTEVRIGLVPAVISPYLMRRTGYSFLRSAF
                     LTAERFDASRAMSVGLVHQVVDASELDEAVEKTVTDLLAAGPEALREARTLVNAVWGR
                     APADVADLTIRTIAKRRLSPEGQEGVSAFLEKRDPRWRI"
     gene            complement(2305..3225)
                     /locus_tag="VI541_02740"
     CDS             complement(2305..3225)
                     /locus_tag="VI541_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HLF69854.1"
                     /translation="MSISKLSADLPTSVTIVEVGPRDGLQNEATPIPVASKVAFIDGL
                     ADAGLKVIEATSFVDPRWVPQLSDADEVLAALQRRPGVRYPVLVPNERGLDRALEAGV
                     NEISIFGSATESFSMRNLDRSRADAVEMFRPVCTRALASGLRVRAYLSMVMGDPWEGD
                     VALAQVVESALALLDLGATELSLGDTIGVGTAGRVQDLIEALLDSGISVEALAVHFHD
                     TYGQGLANVLAALQTGVSIVDSSAGGLGGCPYAKSATGNLATEDLVWMLEGLGIATGI
                     DIKVVALAAKKVCDELSKEVPGRVARALLR"
     gene            complement(3200..>3843)
                     /locus_tag="VI541_02745"
     CDS             complement(3200..>3843)
                     /locus_tag="VI541_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLF69855.1"
                     /translation="RGSVTTTWLSLVERNLPSDASETPDQVLSLAAAAWVDQTTDRSD
                     TDPWASLGAWRLGGEAPMDVVIRVEGAGEATVAIFGGGPYLSQGHAIARDERCHGWSI
                     DGAPASAVIEGAKSFVWFGGGPFEVALGYEERSAEAVAGGHLGAPMPGQILSVRVAAG
                     DRVTKGQALVVMEAMKMEHTIRAPIDGVVAAVLCSEGEQVKRGKTLVDFETER"
BASE COUNT          760 a         1253 c         1136 g          694 t
ORIGIN      
        1 ggggttccgc cactggatcc ttcaccgttg tcgatgacct cgacgtcgat gttggaatcg
       61 ttggatgtca atcgaactgc cacagtggcc tcccccgcgt gacgcatgac gttggtgagc
      121 gcttcctgta tcacccggta aagagccagt tcgactcccg gtgttgttct cggtcggggg
      181 ccggtcacgt gaagggtcac atccatcccg gcggtcctaa gggtgtcgat caaactcccg
      241 atctgatcga tccctggaag aggttccgtc gccacggact cttctgcttc agacgccgcc
      301 acttcagcgg tcgacgagac gaacgagggc cacgcgccct gcgtctcttc gggcttcggt
      361 cgcgctgctt cgatggacgc atctgccgac ctcagcaccc cgattatgct ccgaagttcc
      421 gaaaggctct gtcgcccagt ctcttcggcc gcagcaagag ccgcctgcgc ttcttgtggt
      481 ttcttatcga agaccctgcg ggccgctgcg atctgaaccg tcatgagact cacactgtga
      541 gcaacgatgt catgcagttc tctagcgatg cggactcgct cctccgcgac gatcagtttc
      601 gctgaccgat cacgttctcg tctgccccga tccgctttgc cgaggatctg ggatatgccg
      661 acagcgatca aagtcaacgg ccagaagtag accaactgga tctgccaggc ccccacgctg
      721 tcgagataga agccaacccc aaccgtggca aagatgaggc ccgcaactac cgcgttgcgg
      781 tcgcgttcag agatccgcat gctggaccct cacgtcaccg agcacgaccc cgatctcgag
      841 tttgatgcgc ttgggggcat ccgagaatcc gggcgtagtc ttctcgacac gaccgaagct
      901 ctgcttacgt ccgaagatcg taacctctcc gaccactgct gatgccgaca cagaaacgtc
      961 cgatccgcca ggcaccacga cgcggatgtc accgacgccg accatcacca gtacttccgt
     1021 ggttccctcg ggtatctcaa ggtgagtcag gtccaatctc aggtcgccaa ccccgacgcg
     1081 atagcccgcc ccgatactgc ggaccgacct aggtgcgata tcaaggtcgc cgacgactgt
     1141 gttgaggtga aggtcaccga ggtgctgctc ggtggtccga cgttcgaacg aagagaggtt
     1201 ggccagcagg aggatcagga caagtcccag agcgatcagc ccgccgtggt ggcgtgtgtt
     1261 cgaaacggca atgagaccga gaccaaggac gaccagctct gccgcaagca cctgcgacgt
     1321 cgtgaccgag agggtcccgg tctgctccaa gagccaagcc actcccccaa gcaggagcat
     1381 cacgccgatg acctgacctg ttcggggacg cgaggccgcg ggcctggttg ctgttgcttt
     1441 cgtggtcatg attggaagta gacaggatga ggcatgtccc tgtcgtcatc cgcagggccg
     1501 aaagttgggt acgaccccgg tatgacggcg gtccctagat cctccatcgc gggtcgcgct
     1561 tctcgaggaa cgccgagacc ccctcctgcc cttcagggct taggcgtcgc ttcgcgatgg
     1621 tgcggatcgt caggtcggca acgtccgcgg gggccctccc ccagaccgca ttgaccaacg
     1681 tccgggcttc gcggagcgcc tcagggccag ccgcaagcag gtcggtgacc gtcttctcaa
     1741 ccgcttcgtc gagttcactc gcatcgacga cctgatgcac gagaccgacc gacatagccc
     1801 ggctcgcgtc gaaccgttct gcggtgagaa atgctgacct caggaacgag taacccgttc
     1861 ggcgcatcag atatggagag atgacggcag ggacaagacc gatgcgaacc tcggtgaaag
     1921 cgaaaaccgt ctcggccgac gcgatcgcaa catcagaaca tgcaaccaga ccggtagccc
     1981 cagcgatggc ggcgccgtga acgcgtgcga tggtggctgc cctgcagttg tcgagggcct
     2041 cgaacatcct cgaaagacgc gaagagtcag cggcgttctc ttcaagcgag tacgcagcca
     2101 tgctccgcat ccagtcgaga tcggccccgg cgctgaagac ctttccctca ccggagagga
     2161 ccaacaccct cacgttcgca ggcaagttct cgatggtgga tgtgatctgc tctatgagct
     2221 cggcgttgaa agcgttgcgc acgtccggcc tgttgagagc cagtcgcgcg actccgcgct
     2281 catcaacttg gaagctgacg agactcatcg aaggagagct cgcgcaacgc ggcctggaac
     2341 ttccttgctc agttcgtcgc agaccttctt ggccgcgagg gcgaccacct tgatgtcgat
     2401 accagtcgct atgccgagac cttcgagcat ccagacgagg tcctcggtcg ccagattgcc
     2461 ggtcgcactc ttggcgtatg gacagcctcc caggccccca gcgctcgaat cgacgatgct
     2521 cacccccgtc tgaagagccg caagaacgtt ggccagcccc tgcccatagg tgtcgtggaa
     2581 gtgaacagcg agggcttcaa cggagatacc cgagtcgaga agggcttcga tgagatcctg
     2641 gacccttccc gcggtcccaa cgccgatcgt gtcgccaagc gacaactccg tggcgccaag
     2701 gtcgagcagc gccaaggcag actcgacgac ctgcgctaac gcaacatcgc cttcccacgg
     2761 atcgcccatc accatcgaga gataagcacg aacgcgaagc cccgatgcga gggctctggt
     2821 gcagacgggc cggaacatct cgaccgcgtc ggcacgtgag cgatcgaggt tgcgcatcga
     2881 gaagctctcg gtcgcggaac cgaagatcga gatctcgttc acaccggctt cgagggcacg
     2941 atccagaccg cgctcgttgg ggacgagtac cgggtaacga acccccggtc ggcgctgtag
     3001 cgccgcaagg acctcgtcag cgtcggaaag ctgcggcacc catctcggat cgacgaacga
     3061 ggtcgcctcg atgaccttca gaccagcatc ggctagacca tcgatgaatg cgacctttga
     3121 tgcaacgggg atcggtgttg cttcgttctg aagcccgtcg cgcggaccaa cctcgacgat
     3181 ggttaccgaa gttggaagat cagcgctcag tttcgaaatc gacaagcgtc ttacctctct
     3241 tcacctgttc gccctcggaa cagaggaccg cggccacgac accgtcgatg ggggcacgga
     3301 tcgtgtgctc catcttcatc gcctccatca caacgagtgc ctgacccttg gtgactctat
     3361 ctccggcggc gacgcgcact gagaggatct ggcccggcat gggggccccg aggtggccac
     3421 cggccactgc ctcggcactt cgctcttcgt aaccaagtgc cacttcgaag ggtccacccc
     3481 cgaaccagac gaaagacttt gcgccctcga tgaccgccga ggcgggggcc ccatcgatcg
     3541 accacccatg gcaccgctcg tctcttgcga tggcgtggcc ctgcgacaga tagggtccgc
     3601 ctccgaagat cgccaccgtc gcctctcccg caccttcgac ccgaatgacc acgtccatcg
     3661 gggcctctcc gccgagccgc caagcaccga gcgaagccca ggggtcagtg tcggagcggt
     3721 cggttgtttg atcgacccat gcggccgccg ccagagatag gacctggtcg ggagtctcgg
     3781 acgcatcaga cggaaggttc ctttcgacca gcgagagcca ggttgtggtc actgatccac
     3841 gct
//
LOCUS       DATDOZ010000142         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2741193, whole genome shotgun sequence.
ACCESSION   DATDOZ010000142 DATDOZ010000000
VERSION     DATDOZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741193"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VI541_02750"
     CDS             complement(<1..191)
                     /locus_tag="VI541_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69856.1"
                     /translation="MTARLRPLFVALATIIMSAVLIGLPVAARAAAPNYQTTQLALQP
                     GATRHIDLINPIALVALSW"
     gene            complement(188..2380)
                     /locus_tag="VI541_02755"
     CDS             complement(188..2380)
                     /locus_tag="VI541_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HLF69857.1"
                     /translation="MFAGSRPRSKVARGVNAAITSAVLSIALVFSSSAPASALMLGDG
                     TTPYVFTGAGYGHGVGMSQYGAKGGAVAGASAAQILGHYYTGTSVTMFPQQPVLRIWM
                     TDQTDGTAQVTGTAPFILRRASAPGAPIATVAAGQTAEIRLSGGQFTILVNGTPVGSP
                     IPSTSEKIFAVYDPTTDAGVTASIVRLNEPDKQLRWGRLEFTDASSNLRVVVDALRMQ
                     EYLYGLGEMPSSWPIEALKAQAIAARTYAVRMTDVYPNGKPGCSCNLYGTTADQAYDG
                     YSKETGAYASNWKSSIDQTAGLIVTYNGRAILAQYSSSSGGHTEDSLAISSFPYLKGV
                     PDPWDEAAATGDIRVWRRSFTGADLETWLRADSATNVGNLRGIEVEAPYGVSGRVLDN
                     VVIYGDSGSPKVVSASTFRAVVNAGLSAAGRFSSEQMRSNLFQLGFENYPHGFRGGVF
                     IAGGRNTPLIVTGAGEGGGPHVVVTDVVGRAISGFFAYDPAFSGGVRVALCDLDGDHV
                     QETIITGAGPGGGPHVRTFGSNGSAGKTSFFPYPSGFRGGVYVACGDVDGVAGDELIT
                     GAGPGGGPHVLVFNIDPSTGDPTVTGSFWGFSPSFPGGVRLATGNVAGDTRDEIIVGA
                     GPGGGPHVRILRSDGSEIKGFFAFETSFSGGVYVGSVAVSPTEGYVGVGKGDTGSSEL
                     KVMNSNGDLLWSKTVFGGVMQRGVRVSSVGGAVITSTGNGAWPLVKVL"
     gene            complement(2548..>3564)
                     /locus_tag="VI541_02760"
     CDS             complement(2548..>3564)
                     /locus_tag="VI541_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69858.1"
                     /translation="ALVAFATFKEGFVRHDTHSIIFFTTSAVLGLALAARGHQKWLGA
                     VFIAVAAVAALGAQRQSPSSFFDAVGSARSALTQLTSIATGLGRARLVDDGRTSMSRN
                     FAIDPKIERQLVGRTLDIDPWDASVAWLYPQIRWRPVPAIQSYTAYTRGLDELNAALL
                     RSPRAPELILRRPPSSIDMRYPIFDSPDLQLEMRCRYQPIATRGSWQLLSRLPESRCG
                     QARLIQRVAQQVGSTITIPGTDEHELVFMRVRGFETLALRLMETAFKPPDTSITFDSS
                     DRYRFIPAAGEGSLILNIPAEIDIDATFRPRKHLGTLRFDRAMGAVLGEQIEVEFYAV
                     PIYG"
BASE COUNT          679 a         1061 c         1083 g          741 t
ORIGIN      
        1 ttccagctga gggctaccag agcgatcggg ttgatcagat ctatgtggcg ggtggcaccg
       61 ggctgcagcg ccagttgggt tgtttggtag ttgggggctg cggcgcgggc ggcaacgggc
      121 aggccgatca gcactgctga catgatgatc gttgcaagcg cgacgaagag tggcctaagt
      181 cttgccgtca aagcaccttc actaatggcc aagccccgtt ccccgttgaa gtgattacgg
      241 ctccgccgac gctcgagact cgcacgccgc gttgcatgac accgccgaat actgtcttgc
      301 tccatagtag gtcgccatta ctgttcatca cctttagctc ggacgagcct gtgtcgccct
      361 ttcccacgcc aacgtaaccc tccgtgggag agaccgccac tgagccgaca tagacgcccc
      421 ccgagaagct ggtctcgaat gcgaagaatc ccttgatctc ggacccatca gatctcagta
      481 tccgcacgtg tgggcctccg ccggggcccg caccgacgat gatctcgtcc ctggtatcac
      541 ctgcgacatt gcctgttgct aaccgaacac ctccagggaa gctggggctg aacccccaga
      601 agcttccggt gacggtcgga tcgcctgttg atgggtcgat gttgaagacc aggacatgtg
      661 gccctccccc tggacccgca cccgttatga gctcatctcc agcaactccg tcaacgtcgc
      721 cgcacgcaac atagacccct cctcggaagc cggaggggta ggggaagaat gaggtcttgc
      781 ccgcagaccc gttactgccg aaggttctga cgtggggccc cccacccggc cctgctcccg
      841 tgatgatcgt ctcttgaaca tggtcaccgt cgaggtcgca tagagccacc cgaaccccac
      901 ctgagaaggc cgggtcgtat gcgaagaatc ctgagatcgc ccggcccaca acgtcggtga
      961 cgacaacgtg gggacctccg ccttcgccag cccccgtgac tatcaatgga gtgttgcgac
     1021 ctccagcgat gaagactcct ccgcgaaatc cgtgtggata gttctcgaac cctagttgaa
     1081 agaggttgga gcgcatctgt tcgcttgaga agcgccccgc cgccgagagt cctgcattaa
     1141 ctaccgctcg gaatgtcgag gcgctcacaa cctttggaga ccccgagtct ccatagatca
     1201 cgacgttgtc gagcacgcgc cctgagactc cgtagggggc ctcgacctcg atgccgcgaa
     1261 ggttgccgac atttgtggct gagtcggctc gaagccaggt ttctaggtcg gctccggtga
     1321 aggagcgtcg ccaaactctg atgtcgccag tggctgcggc ctcgtcccaa gggtcgggaa
     1381 cgcccttgag ataggggaag gaactgatgg ccaagctatc ctcggtgtgg ccaccgctcg
     1441 atgagctgta ttgggcaagg atcgctctcc cgttgtaggt cacgatcagt ccagcggttt
     1501 ggtcgatgga cgacttccag ttggatgcat atgcgcccgt ctctttcgag taaccgtcgt
     1561 atgcctgatc ggcggttgtg ccatagaggt tgcagctgca gccgggctta ccgttggggt
     1621 acacatcggt catccgtacg gcataggtgc gtgccgcgat ggcctgggct ttgagggcct
     1681 cgatcggcca tgacgagggc atctccccga gaccatagag gtactcctgc atgcgaaggg
     1741 catccaccac cactctgaga ttggaactag catcagtgaa ctccagcctg ccccacctga
     1801 gctgtttgtc cggttcgttc aaacggacga tgcttgccgt tactcccgca tcggtggtcg
     1861 ggtcatagac cgcaaatatc ttttcgctgg tcgatgggat cggactgccg acgggtgttc
     1921 cgttgaccag gatcgtgaac tggccgccag agagcctgat ctccgcagtc tgtcccgcag
     1981 cgactgttgc gatcggcgcg cccggtgcgc tggctcgcct taggatgaag ggcgctgttc
     2041 ccgtcacctg cgcggtgcca tcggtttggt cggtcatcca gattcgaaga accggctgct
     2101 gcgggaacat cgttaccgag gtccccgtgt agtagtggcc gagtatctgc gcagcactag
     2161 cccctgctac ggcgccaccc tttgctccgt actgactcat gcccactcca tgaccgtagc
     2221 cggcgccggt gaagacgtag ggcgtggttc cgtcaccgag catgagcgcc gaggctggag
     2281 cagaggaaga gaaaaccaat gcgatggata ggaccgccga tgtgatcgcc gcgttcaccc
     2341 cgcgggctac cttggagcgg ggtcttgacc ctgcaaacat ccatacctcc cgtttacgtg
     2401 ctgcggcacc cgcgagcgga tgctgcgcct caccccccgg aaggctatct gccctgctct
     2461 gaatcattcg gaaaggcgtc tgacatgctt gaatccacgg cgagccgccc aaagattgta
     2521 gccgagtcgt ccgtcgtgcc tggggtttca gccgtagatg ggcactgcat agaactcgac
     2581 ctctatctgc tctccaagaa cggctcccat cgcacgatca aatcgcaggg tgcccaaatg
     2641 ctttcgaggt cgaaatgtcg catctatgtc gatctcggct ggaatgttca atatgagcga
     2701 accttctccc gctgctggga tgaagcggta tctgtcgctc gagtcgaagg ttatcgatgt
     2761 gtcgggaggt ttgaaggctg tttccatcag tcgcaacgcg agggtctcga atccccgaac
     2821 cctcatgaag acgagctcgt gctcatcagt gccggggatc gtgatggtgc ttccgacctg
     2881 ctgagcgaca cgttggatca gtcgagcctg accacagcgt gactcaggca accgagagag
     2941 cagctgccag gatccccgag ttgcgatggg ttggtagcga catcgcatct cgagttgcag
     3001 gtcgggggag tcgaagatcg ggtagcgcat gtcgatcgac gatggaggtc ttcgaaggat
     3061 gagttcgggc gcccttggcg atcgcaggag tgctgcgttc agttcatcga gtccgcgggt
     3121 gtacgcggta tagctctgga tggcggggac aggacgccat cggatctgag gatagagcca
     3181 tgcgacgctg gcatcccacg gatcgatgtc gagggttctt ccgacgagct gtctctcgat
     3241 cttgggatcg atcgcgaaat ttctcgacat gctggttcgc ccatcatcaa cgagccttgc
     3301 ccggcctaaa cctgttgcga tgcttgtgag ttgagtaagc gctgatctag cgcttcctac
     3361 ggcgtcgaag aagctcgacg ggctctgtct ctgggctccc agggcggcaa ctgcagctac
     3421 tgcgatgaag actgcgccca gccatttctg atgcccgcgg gccgccaagg caagcccgag
     3481 cacggcactg gtggtgaaga agatgatcga gtgggtgtcg tgtctgacga atccctcttt
     3541 gaatgtcgcg aatgccacta gtgc
//
LOCUS       DATDOZ010000143         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_637086, whole genome shotgun sequence.
ACCESSION   DATDOZ010000143 DATDOZ010000000
VERSION     DATDOZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637086"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..510
                     /locus_tag="VI541_02765"
     CDS             139..510
                     /locus_tag="VI541_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69859.1"
                     /translation="MATLHRTFGLGVGFAFLLLGLLGLIAWFRNRDPGRIFWGLLAGG
                     QVGLVVQLLIGVATFLTTKGPRPHALHFLYGGFPIFVLFFAHRYSSRFKGLEWLAFAF
                     AGLFIFGLQLRGCMTGGGCGF"
     gene            569..1735
                     /locus_tag="VI541_02770"
     CDS             569..1735
                     /locus_tag="VI541_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HLF69860.1"
                     /translation="MAPEVEIGIGKAARRAYGFDDIAIVPSRRTRDPDDVDISWEVDG
                     FRFELPLLAASMDAVVSPTSAVAVGALGGLAVLNLEGLHTRYEDAESVLEEIAALPQE
                     TATARMQQLYQAPVKEELVYKRITEIKGAGVIAAASLTPQRVEEFSKVVLEAELDILV
                     IQGTVVSAEHVSSQTEPLNLKRFIATFDLPVIVGGCASYQAALHLMRTGAIGILVGVG
                     PGAACTSRQVLGIGVPQATAIADAAAARMTHLDETGRYVHVIADGGMRTGGDIAKAIA
                     CGADAVMIGSPLARSTEAPGRGYHWGMATFHSALPRGVRVSSSPLGTLEEILLGPTSV
                     NDGTMNLFGALRNSMATCGYESVKEFQKAEVVIAPAITTEGKYLQRQQGIGQGS"
     gene            1741..>2447
                     /locus_tag="VI541_02775"
     CDS             1741..>2447
                     /locus_tag="VI541_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69861.1"
                     /translation="MSAVLRKVVGWVQEHKGRFGGALLALIIIQSFAAYSALTTSTTV
                     TRTDALAEFRLRQSQGSASPDPASSQAGPGPSGAASAPGGGPPPGGGSGGTKTASPQP
                     GGPQCDWICPTSYTVPDPGVYEYFQCGRAAGQCDGSASEPRGHEVLGVVGRDLPDRGQ
                     RIVATQDNTHWNNIHRYSDEHREEFDLAVSPSELVNSRYKVDITVGGHTERNDIRQSP
                     PYRLVRFPVVLGDSWTG"
BASE COUNT          443 a          773 c          758 g          473 t
ORIGIN      
        1 ttttgatgtg tcaaacatgc cgtcttgctc tcaaaccgca gcgcacctat cctcaggaag
       61 ggccgtcgag tcgtaaacct ccggggaaga aggctcggct ggcccatctc gtcgccatct
      121 cgcgttagca tgccgggcgt ggcaaccctt catcgaacat tcgggctcgg agttggcttc
      181 gcattccttc ttcttgggct cctcgggcta atcgcgtggt tccggaaccg cgacccgggc
      241 aggatcttct ggggcctgct agccgggggg caggtcgggc tcgtcgtgca gctcctcatc
      301 ggcgtcgcaa cattcctcac cacgaagggg ccccgccctc acgctctcca cttcctatat
      361 ggcgggttcc cgatcttcgt tctctttttc gcccaccgct actcgtcacg cttcaagggg
      421 ttggagtggc ttgccttcgc attcgccggt ctcttcatct tcggacttca gctgcgtggg
      481 tgcatgaccg gtggcggctg cggattctaa gagttacgac gcctgataaa gtttgaggat
      541 ccaccccgca aacagaaggg ttcacacatt ggctcctgaa gtcgagatcg gcatcggtaa
      601 agcggctcgt agagcctacg ggttcgacga catcgcgata gttccatcca gacgtacccg
      661 tgaccccgac gatgtcgata tctcttggga ggtcgatggc ttccgcttcg agcttcccct
      721 gctggcggct tcgatggatg ccgtggtctc gccgacgagt gccgtggctg ttggcgcact
      781 ggggggcctt gcggtcctca atctcgaagg ccttcacacg cgctacgaag atgctgagtc
      841 ggtcctcgaa gagatcgccg cgcttccgca ggaaacagcg acagcgcgta tgcagcagtt
      901 gtaccaagcg ccggtcaagg aggagctcgt ctacaagcgg ataaccgaga tcaagggtgc
      961 cggagtcatc gccgccgcca gcctcacgcc gcagcgagtc gaggagttct ccaaggtcgt
     1021 tctcgaagca gagctcgaca tcttggtgat ccaagggacg gttgtctcgg ccgagcacgt
     1081 ttcgagtcag accgagccgc tcaacctcaa acgattcata gcgacattcg accttcctgt
     1141 gatcgttgga ggttgcgctt cgtatcaggc ggctcttcat ctgatgcgga cgggtgccat
     1201 cggcatcctc gtaggcgtgg gcccgggggc cgcgtgtact tcacgacagg tcctcggtat
     1261 cggggtcccc caagcaactg ccatagccga cgcggccgcc gcgcgcatga cccatctcga
     1321 tgaaaccgga aggtacgtgc acgtcatcgc ggacggtggg atgcgcaccg gcggtgacat
     1381 cgcaaaggcc atcgcatgtg gtgctgacgc ggtgatgatc ggttcgccgc tcgcacgaag
     1441 caccgaggcc cccggtcgcg gataccactg gggcatggcg accttccact cggcccttcc
     1501 ccgcggcgtc cgagtcagct cgtcgcccct cggcacgctc gaggagatcc tgcttggccc
     1561 tacgagcgtc aacgacggaa ccatgaacct gttcggtgct ctgcgaaact ccatggctac
     1621 atgtgggtat gagtcggtca aggagttcca gaaggcggag gttgtcatcg ctccggcgat
     1681 cactaccgag ggaaagtatc tgcagcgaca gcaggggatc ggccagggaa gctgagtttc
     1741 ttgtccgcag tcctgcgcaa agtcgtcggg tgggtccaag aacacaaggg gagattcggc
     1801 ggagccctcc tcgcgttgat catcatccag agtttcgcgg cctactctgc ccttacgacc
     1861 tcaacgacag tcactcgcac cgacgcgctg gcagagtttc gccttcggca gtcgcagggc
     1921 agtgccagtc cggatccggc atcatctcag gcgggccccg ggccctcagg cgcggcatcc
     1981 gctccaggcg gtggtccacc ccctgggggc ggaagcggtg gcaccaagac tgcatcaccg
     2041 caaccgggtg gccctcagtg cgactggatc tgcccgacga gctacacggt gcccgaccca
     2101 ggggtctacg aatacttcca gtgcggacgt gccgcggggc agtgtgacgg ttcggcttcg
     2161 gagccaagag gccatgaggt cctcggggtc gtcggccgcg acctccctga tcgagggcag
     2221 cggatcgtcg cgacgcagga caacacccac tggaacaaca tccaccgcta ctcggacgaa
     2281 catcgcgagg agttcgacct cgcggtaagc ccatcggaac tggtgaacag ccgctacaag
     2341 gtggacatca cggtcggagg gcacaccgaa cgcaacgaca tcaggcagag cccgccctat
     2401 cgcttggtgc gcttccccgt cgttctgggc gacagttgga ctggcca
//
LOCUS       DATDOZ010000144         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_639383, whole genome shotgun sequence.
ACCESSION   DATDOZ010000144 DATDOZ010000000
VERSION     DATDOZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639383"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="scpB"
                     /locus_tag="VI541_02780"
     CDS             complement(<1..413)
                     /gene="scpB"
                     /locus_tag="VI541_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HLF69862.1"
                     /translation="MTQSEMRRALEAVLFIADESIPSADLAVLFEVPTARIEEEMSAL
                     ADDLEQSGRGVVLRQVAGGWRMSTHPDVSQYLERFVAEGRSGRISNAALETLAIVAYR
                     QPVSRATIGEIRGVASEGVLKTLVARGVIEEVGRD"
     gene            complement(421..1152)
                     /locus_tag="VI541_02785"
     CDS             complement(421..1152)
                     /locus_tag="VI541_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HLF69863.1"
                     /translation="MGYAVKLEIFEGPLDLLLHLVSKERLDVADVSIATITDDYLRAV
                     RAMGEVDLETASAFLVLAATLLELKSLKLLPRTQIDDPELASLLEERDHLLHRLIEYS
                     TFRNAAGTFEEMLRATEDFHSRRAELPVELVPALPDILEGLTALRLARAAAKSLAPKP
                     KVSLEATHVTPIRVSVGEMVELLSSMFQSGGVTSFTEVRRGAANRIEVIVRFLALLQM
                     YKDQAIELEQEKPFDEIIVRWRQPL"
     gene            complement(1211..2203)
                     /gene="trpS"
                     /locus_tag="VI541_02790"
     CDS             complement(1211..2203)
                     /gene="trpS"
                     /locus_tag="VI541_02790"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLF69864.1"
                     /translation="MTKVFSGVQPTSDVPHLGNYIGAFRNWVTLQDRYQSLYCIVDLH
                     SMTVAWDPTILARRTLQSAASLIALGIDPERSVLFVQSDVREHTELAWILNCIARMGE
                     LNRMTQFKDKARNQEASVGAGLFAYPVLMAADVLVHRAHLVPVGDDQKQHLELMRDIA
                     ERFNRSFGETFPIPDPLIPDVGGRVMSLDDPTKKMDKSSDRPGSLIWMTDPPEVVRSK
                     IAKAVTDSGREIVYGPEKPALSNLLDIFSAVSGREVSDIQESFVGKGYADLKAELSDA
                     LIEFLSPFQERYSELMKDTAELGKLLDRGADSARQEAAETMKIVRERVGLRASS"
     gene            2253..>2461
                     /locus_tag="VI541_02795"
     CDS             2253..>2461
                     /locus_tag="VI541_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017868948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLF69865.1"
                     /translation="MGPLQLLLDGHFLELFAILLVLVIAITIHEFGHAFAADVQGDRT
                     ARLAGRLTLNPLSHLDPLGSVLLVLA"
BASE COUNT          458 a          788 c          711 g          504 t
ORIGIN      
        1 tcatcgcgcc cgacttcctc gatgacgccg cgcgccacca aggtcttgag gaccccctca
       61 gaagccacgc ctcttatctc accgatggtc gcacgagaga cgggctgtcg ataggccact
      121 atcgcaagtg tctcgagggc cgcgttcgat atgcgtccgc tcctcccctc ggcgacgaac
      181 ctctcgagat actgactgac atctgggtgc gtggacatgc gccatccgcc agcaacctgc
      241 cgtagaacca ctccccggcc cgactgctcg agatcatcag cgagcgcgga catctcttct
      301 tcgatccgag ccgtggggac ctcgaacaga actgcgaggt cagccgacgg gatcgattca
      361 tccgcgatga acagcactgc ttcgagggct cgcctcatct cgctttgcgt catttcagcc
      421 tcatagtggt tgtctccacc ggacgatgat ctcgtcaaag ggtttctcct gctcgagttc
      481 gatggcctga tccttgtaca tctgcagcag cgccaggaac ctcacgataa cctcgatccg
      541 gttcgctgcc cctcgacgca cctcggtgaa agaggtgacg cctccgctct ggaacatcga
      601 gctgagcaac tcaaccatct cgccgacgct caccctgata ggcgtcacat gagttgcctc
      661 gagcgaaacc ttgggcttgg gagcaagact cttcgcagcc gcacgtgcca gccgcagggc
      721 cgttagccct tcgaggatgt cggggagcgc tgggaccaac tcgaccggaa gctctgctcg
      781 ccgcgagtgg aaatcctcgg tagcgcgcag catctcttcg aatgttcctg ctgcgttgcg
      841 gaatgtggag tactcgatga gtcggtggag aaggtggtca cgctcctcga gcagtgatgc
      901 aagctcggga tcatcgatct gtgtgcgagg caagagcttt agagacttga gttcgagcaa
      961 tgttgcggcc agcaccagga aggccgaagc cgtctcgagg tcaacctcac ccatcgccct
     1021 gacagccctc aggtagtcat cggtgatggt tgcgatcgac acatccgcca catccagacg
     1081 ctccttcgag acgagatgga gcagcaggtc gaggggcccc tcgaatatct ctagcttgac
     1141 ggcgtagccc acgtcgatcc ctcctggacc caatataacc agaggggcgc ttggcatcgg
     1201 ggcatttggt ctagcttgac gctcgaagcc cgaccctctc tcggacgatc ttcatcgtct
     1261 cagccgcctc ttgtctcgcc gagtctgccc cccggtcgag aagcttgccc agctccgcag
     1321 tatctttcat cagttccgag tacctctcct gaaagggaga aaggaactcg atgagggcat
     1381 ccgacagctc tgccttcagg tcggcgtacc ccttgcccac gaaactctcc tggatgtcag
     1441 agacctcgcg acccgagaca gcggagaaga tgtcgagaag gttcgaaagc gctggcttct
     1501 cggggccata gacgatctcc cgacccgagt ccgtgaccgc cttcgctatc ttcgagcgca
     1561 ccacctctgg cgggtctgtc atccatatga gactcccagg gcggtcgctc gacttgtcca
     1621 tcttcttggt tgggtcatcg agcgacatca cccggccccc gacatcaggg atcagtgggt
     1681 ccggtatggg gaaggtctcc ccgaaggatc ggttgaatcg ttcggcgatg tctcgcatca
     1741 gttcgaggtg ctgcttctga tcgtcgccca cgggcacaag gtgggcgcgg tgcacgagca
     1801 cgtcggccgc catcaggacc gggtaggcga agagccccgc ccccacactg gcctcctggt
     1861 tgcgggcctt gtccttgaac tgggtcatgc ggttcagctc tcccatccgc gcgatgcagt
     1921 tcaggatcca tgccagctcc gtatgctcac gcacgtcgga ctgcacgaag aggaccgacc
     1981 gctcggggtc tatccctaag gcgatcaggc tggccgcaga ctgcagagtg cggcgggcga
     2041 ggatggtcgg atcccacgca actgtcatcg agtgcagatc cacgatgcag tagaggctct
     2101 ggtatctgtc ctggagtgtg acccagttac ggaatgcccc gatgtagttc ccaagatgtg
     2161 gaacgtctga ggtcggctga actcctgaga agaccttcgt catgggcgtc agtttcgcat
     2221 aacaaagggc gtacaccgtc tatgctcgac ggatgggtcc cctacagctc ctgctcgatg
     2281 gccacttcct cgagctcttc gcgatcctgc tggttctggt catcgcgatc acgatccatg
     2341 agtttgggca cgccttcgca gccgacgtcc aaggtgaccg taccgcccgg ctcgcggggc
     2401 gcctcaccct gaaccccctg tcgcacctcg accccttggg ttcggtcctg ctggtgttgg
     2461 c
//
LOCUS       DATDOZ010000145         3292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2472591, whole genome shotgun sequence.
ACCESSION   DATDOZ010000145 DATDOZ010000000
VERSION     DATDOZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472591"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VI541_02800"
     CDS             <1..1142
                     /locus_tag="VI541_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLF69866.1"
                     /translation="VGRLFKGLQGVIKARGVRVVQGHGKLTSADTIEVKDETIRARNI
                     VLATGAEPRSLPFIKVDGKAFITSDHALTLDDIPSSVIVIGAGAVGLEFASVYRSFGA
                     EVTILEALPRLAPGEDEEVSKEVTRAFTKRGIRSFTGVKVVSAETSSGRASITYQGAD
                     GKAVTVAADRCLVAIGRGPNSSGMGFEEAGVTLERGFVVVDGSCRSGVADVWAVGDLI
                     AQPSLGLPFPHFQLAHVAFAEGIHVAEQIAGIDSTPVNYVGVPRCTYSFPEIASVGLT
                     EEQAKAAGNDVQIARFPWAASGKATILGETSGFVKVVARVDGPVLGVHMVGPRVTELI
                     AEAQIITNWDAYPADVAALIHPHPTLSEALGESMLHLTGKQLHGA"
     gene            1161..>3292
                     /gene="aceE"
                     /locus_tag="VI541_02805"
     CDS             1161..>3292
                     /gene="aceE"
                     /locus_tag="VI541_02805"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HLF69867.1"
                     /translation="MPYDVFKEQLPDPYPEETAEWLAALNDVIENQGKARARYLLSRV
                     LLQARERQIGLPSMVQTDYVNTIPPEQEPYYPGNEALEFRIRQFIRWNAAVMVLRANK
                     LFAGIGGHLATYASAAGLYEVGFNHFFRGKDHPGGGDQVFLQGHGAPGIYARAYLEGR
                     ITEQQLEHFRREALWEGGLSSYPHPRLMPEFWEFPTVSMGLSALNAIYQARFNRYLGH
                     RGIKDTSDQRVWAFLGDGEMDEPESVAALHLAASEGLDNLTFVVNCNLQRLDGPVRGN
                     GKIIQELEARFRGAGWHVIKVIWGREWDDLLARDTEGILLNRMNQTLDGEFQRYSVES
                     GAYIREHFFGSDARLKQMVSHLSDEDLWKLKRGGHDYRKVYAAYQVATELKDRPVAIL
                     AKTVKGWALGPEVEARNATHQIKKMTEEELRRLAERLNIEVSEKKLKGEEPPFIHPGE
                     DSDEVQYLQEHRRRLGGYIPERRAKAKPLVLPGDLVYSVFDAGTAEAKREVSTTMAFV
                     ALLRDLMKDREVGERVVPIIPDEARTFGMESLFKDFKIYAPGGQLYEPVDYNMLLSYR
                     ESRDGQLLEEGITEAGGMASFTAAATAYATHGQHMIPFFMFYSMFGFQRVGDLIWALA
                     DARGRGFLLGCTAGRTTLNGEGLQHADGHSPLLASVVPNCMVYDPAFAWETAAIVREG
                     LQRMIDDGEDIFYYLTLYNENYVMPAKPA"
BASE COUNT          633 a         1010 c         1020 g          629 t
ORIGIN      
        1 tcgtcggaag actcttcaaa ggtctgcagg gtgttatcaa ggcccgcggc gtccgagtcg
       61 ttcagggcca cgggaagctc acatcagccg acaccatcga ggtcaaggac gagaccatcc
      121 gtgctcgcaa catcgttctg gccacggggg ccgagccccg ctcgcttcca ttcatcaagg
      181 tcgatggcaa ggctttcata acctccgacc atgcgctcac gctcgacgac atcccgtcct
      241 cggtcatcgt catcggtgcg ggggcggtcg gcttggagtt cgctagcgtc tatcgctctt
      301 tcggtgcaga ggtcacgatc ctcgaagcgc tgccgcgcct agccccaggt gaggacgagg
      361 aggtttcgaa ggaggtgacg cgtgccttca cgaagcgcgg gatccgctca ttcaccggag
      421 tcaaggtcgt ctccgccgag acctcgtctg ggcgcgcgtc aatcacttac caaggcgcgg
      481 atgggaaagc agtcactgtt gcagccgacc gttgtctagt ggcgattgga cgggggccga
      541 actcatccgg catgggtttc gaggaggcgg gcgtgacgtt ggagcgcggc ttcgtcgtcg
      601 tcgacggatc atgtcgcagt ggcgtagcgg atgtctgggc ggttggcgac ctcatcgccc
      661 agccctcgct cggtctccca ttcccacact tccaactcgc gcatgtcgcg ttcgctgaag
      721 ggatccatgt ggccgagcag atcgcaggta tcgatagcac ccctgtgaac tatgtcggcg
      781 ttcctcggtg cacatactca ttccccgaga tcgcttcggt cggcctgact gaagagcagg
      841 ccaaggcggc gggtaacgat gtgcagatcg cccggttccc ctgggccgca tcgggcaagg
      901 cgaccatcct gggcgagaca tccgggttcg tgaaggtcgt cgctcgtgtc gatggccctg
      961 tgctcggtgt gcatatggtg gggccgcggg tgaccgaact catagccgag gcccagatca
     1021 tcaccaactg ggatgcctac cccgccgatg tcgccgcgct gatacatcct cacccgaccc
     1081 tctccgaggc gttgggggag tcgatgctcc atctcacagg taaacaactc cacggcgcct
     1141 aacccggtga taatgggtgt atgccgtatg acgtcttcaa ggagcagctg cctgatccgt
     1201 atccagagga gacggcggag tggctagctg ccctcaacga cgtcatcgag aatcagggca
     1261 aggcccgcgc tcgctacctt ctctcccgcg tcttactcca agcccgcgag cggcagatcg
     1321 gtctcccctc gatggtgcag accgactatg tcaacaccat cccgcccgaa caggagccgt
     1381 actaccccgg taacgaggcc ctcgagttcc ggataagaca gttcatccgt tggaacgcag
     1441 cggtgatggt gcttcgcgcc aacaaactgt tcgccgggat agggggtcac ctcgcgacct
     1501 acgcctcggc agccgggctc tacgaagttg gcttcaacca cttcttccgc ggcaaggacc
     1561 acccaggtgg cggcgaccag gtcttcttgc aggggcatgg ggcccccggg atatacgcga
     1621 gggcctacct cgaagggcgc atcaccgaac agcagctcga acacttccga cgcgaggccc
     1681 tttgggaggg cggactttcg tcctatccgc atccgcgctt gatgccggag ttctgggagt
     1741 tcccgacagt ctcgatgggg ctctccgcgc tcaacgccat ctaccaggct cgattcaata
     1801 gatacctagg ccatcgcggc atcaaggaca catctgatca gcgcgtctgg gcttttcttg
     1861 gcgacggcga gatggacgaa cccgagagcg ttgccgctct ccaccttgca gcaagtgagg
     1921 ggctcgacaa ccttacgttc gtcgtcaact gcaacttgca gaggctcgac ggcccagtgc
     1981 gcggcaacgg caagatcatc caggagctcg aagcgcgatt tcgcggtgcg ggctggcatg
     2041 tcatcaaggt catctggggc cgcgagtggg acgaccttct ggctcgcgac accgagggga
     2101 tccttctgaa tcggatgaac cagactcttg atggcgagtt ccaacgctat tcagtggagt
     2161 ctggtgcata catccgcgaa cacttcttcg gctccgatgc acgtctcaaa cagatggtgt
     2221 ctcacctatc cgatgaagat ctttggaagt tgaagcgcgg cgggcatgac tatcgcaagg
     2281 tctatgccgc ctaccaggta gcgacagagt tgaaggaccg gccggtggcg atcctcgcga
     2341 agacggtcaa gggatgggcc ctcggccccg aggtcgaggc acgcaacgcg acccaccaga
     2401 tcaagaagat gacggaagag gagctgcggc gcctcgccga gcggctcaat atcgaggtct
     2461 ccgagaagaa actcaagggc gaggagccac ccttcatcca ccccggcgag gactcagacg
     2521 aggtgcagta cctccaggag catcgccgtc gcctgggagg ttacataccg gagcgccgcg
     2581 ccaaggccaa accactggtt ctgccaggcg acctcgtcta ctcagtgttc gatgcaggaa
     2641 ccgctgaagc caagcgagag gtttcgacga cgatggcctt cgttgctctg ctgcgtgacc
     2701 tcatgaagga ccgcgaggtg ggggagcgag tcgttccgat aatccccgac gaagcacgca
     2761 ctttcggcat ggagtcgcta ttcaaagact tcaagatcta cgcgcccggg ggccagttgt
     2821 atgagccggt cgactacaac atgctcctct cgtatcgcga gtcgcgcgat ggtcagctgc
     2881 tcgaagaggg gatcaccgag gccggaggca tggcttcgtt cacggccgca gcgacagcgt
     2941 atgcgactca tgggcagcac atgatcccct tcttcatgtt ctattcgatg ttcggtttcc
     3001 agcgagtcgg tgacctgata tgggcgttgg cagatgcgcg tgggcgcggg ttcctcctcg
     3061 ggtgtacggc ggggcgcacg acgttgaacg gcgagggact tcagcacgcc gatggccact
     3121 cgcctctgct ggcctccgtg gttccgaact gcatggtcta cgacccggcc ttcgcgtggg
     3181 agaccgcagc catcgtgcgc gaaggtctgc agcggatgat cgacgatggc gaggacatct
     3241 tttactacct gactctttac aacgagaact acgtgatgcc cgccaagccc gc
//
LOCUS       DATDOZ010000146         2518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2989850, whole genome shotgun sequence.
ACCESSION   DATDOZ010000146 DATDOZ010000000
VERSION     DATDOZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989850"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VI541_02810"
     CDS             <1..225
                     /locus_tag="VI541_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HLF69868.1"
                     /translation="LTVSKGPEPFSMPDVRGRKCSDAKKQLEALGLKVVVKSSAGKQT
                     GCSADRIAAQDPVPDATVRKGDEATLYILT"
     gene            complement(210..1232)
                     /locus_tag="VI541_02815"
     CDS             complement(210..1232)
                     /locus_tag="VI541_02815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLF69869.1"
                     /translation="MLATCVAPALAWEAAPAAAAAPPLGMSSGILVDLTSGKILWARD
                     DESPRPPASLTKVLTALVVMRNARLDDKVVITPEARQAPGGRTYAEAGWTFSVRDALW
                     GLLLQSGNDTAIALAQKVSPDGSIDGFARMMDATAKEVGATHSHFGNPHGFDQEGHLS
                     TARDLALITMAALKDPVIAEMVATRVHQVPWGDGTMHTYINHNDMLVRYPGTIGVKTG
                     FTAGAGRSLISAVRRGNDVLIAVVMNSPDHYAESKALYDWSFANLGGLRAHPIGVLTS
                     NPRSELLPGEASTLPKVEQLQPEVLAEEEGRHVTSGPLLAPVIALVIALAAASVFRRR
                     SDQVSM"
     gene            1320..2183
                     /locus_tag="VI541_02820"
     CDS             1320..2183
                     /locus_tag="VI541_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLF69870.1"
                     /translation="MSTKRARWLLVPAIIAVAWSAPLVRLAEGVPPLAIAFWRTFAAT
                     VVIAPFALVRGRADPPSRKQVKMMVLSGAALAIHFASWIASVRLTSIASSVLLVVSAP
                     VFVALGTTLLGRAPARRVWLGIAIATTGAAVIAGSDLNVGGGLKGDGLAVVGAIAAAI
                     YLLIGRGSRRHVSLLTYAGIVYGSCAALLGVTMLLTGTPFTGYSSKQFLLVAAIAIGP
                     QLIGHTTFNFVLDRLDAVEVAVAIMAEPIGATLIAFALFGELPGILVVPGGLLLLAGI
                     LVSIRASKGSV"
     gene            complement(2085..>2518)
                     /locus_tag="VI541_02825"
     CDS             complement(2085..>2518)
                     /locus_tag="VI541_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69871.1"
                     /translation="RYGLSGVVNVIEKTGPIRSLGRSRELLRRRGFDYFLLLLLLLAV
                     SLVAGLVLGGPRLIVAASSDLAKPTVGLAPAAAMVVGVSAYLSTIVTVLVSWGSRTNY
                     YLHCIIEDSSDRPLASSDADQDPGKEQETSGNDEDPWKLTE"
BASE COUNT          454 a          786 c          778 g          500 t
ORIGIN      
        1 ctcacagtga gcaaagggcc agagcctttc tcgatgccag atgttcgtgg ccgcaagtgc
       61 tctgacgcca agaagcaact cgaagccttg ggcctcaagg tcgtcgttaa gtctagtgct
      121 ggtaaacaga cgggatgttc cgcagaccgc atcgcggcgc aggatccggt ccccgatgcg
      181 acggtgcgaa agggcgatga agcgaccctt tacatactga cctgatcgga tctgcgccga
      241 aagacagagg cagcagcgag agcgatcacc agagcgatga ccggtgctag cagagggcct
      301 gatgtcacgt gccgaccttc ctcctcggcg agtacctcgg gctgcagttg ctcgaccttg
      361 ggcagtgtgc tcgcttcccc aggtaacagt tcgctcctgg ggttgcttgt aagaactccg
      421 atcggatgcg cacgaaggcc ccccaggttg gcgaaactcc agtcgtatag ggccttggac
      481 tcggcgtagt ggtcggggga gttcatcacc acagcgatga gcacgtcgtt gccgcgccgc
      541 actgctgaga tgaggctgcg accagccccc gccgtgaagc cagtcttcac gccgatggtc
      601 cccgggtagc gcacgagcat gtcgttgtgg ttgatgtagg tgtgcatcgt gccgtcgccc
      661 caggggacct gatgaacacg cgtcgccacc atctcggcga tcacagggtc cttcagagcg
      721 gccatcgtga tcagggcaag gtcacgcgct gtgctgaggt gaccttcctg gtcgaagcca
      781 tgtggattgc cgaagtggct gtgggtcgcc ccaacctcct tggcggttgc gtccatcatc
      841 cgtgcgaatc cgtcgatcga accgtcgggg ctcaccttct gagcgagggc gatcgcggtg
      901 tcattcccgc tctgcaggag cagtccccag agtgcatctc gcaccgagaa ggtccacccc
      961 gcctcggcgt aggttcggcc cccgggggcc tgccgagcct caggcgtgat cacgaccttg
     1021 tcgtcgagcc tcgcattcct catcaccacg agcgcagtga gcaccttggt cagactcgca
     1081 ggcgggcgcg gcgactcatc gtccctcgcc caaagtatct ttcccgatgt gaggtcgacc
     1141 aggatccccg aggacatccc cagaggtggt gcggccgcgg ccgctggggc agcctcccaa
     1201 gcaagtgcgg gagcaacgca agtcgctaac atagcggccg cgacgagccc gcatcgaaat
     1261 agtttgaaca tccggaggtc aacagtacgt aaggcgcgcc gcagtagcaa gtacccggca
     1321 tgagtaccaa acgcgcccga tggctgttgg tcccggcgat catcgctgtg gcttggtcgg
     1381 ccccgctcgt gagactagcg gaaggcgttc ccccgctcgc gatcgccttt tggcgcacct
     1441 tcgccgcgac cgtagtcatc gctccattcg ctctggtccg tggacgcgct gatccgccat
     1501 ctcgcaaaca ggtgaagatg atggtcttgt cgggcgcggc ccttgcgatc cacttcgcat
     1561 cctggatcgc ctcggtgcga ctcacgagca tcgcttccag cgttctgctg gtcgtgtctg
     1621 ccccggtgtt cgttgcgctc ggtaccactc tgctcggacg ggccccagca agacgagttt
     1681 ggctcggtat agcaattgcg acaacggggg ccgcggtcat cgcgggttct gatctaaacg
     1741 tcggtggtgg actaaaggga gatggactgg ccgtcgtcgg tgctatcgct gcggccatct
     1801 atctgttgat cggccgggga tcgcgacgac atgtatcact gctcacatat gcgggcatcg
     1861 tctacggctc ctgcgcggct ctcctcggcg taacgatgtt gctaaccgga acgccattca
     1921 cgggttactc gtcgaagcag ttcctcttgg ttgcggcgat cgcgatcggt ccccagctga
     1981 tagggcacac gactttcaac ttcgtgctcg accggctcga tgcggtcgag gtcgccgttg
     2041 cgatcatggc agagcctatc ggcgccacct tgatcgcttt cgctctattc ggtgagcttc
     2101 cagggatcct cgtcgttccc ggaggtctcc tgctccttgc cgggatcctg gtcagcatca
     2161 gagctagcaa ggggtcggtc tgatgagtcc tcgatgatgc agtgcaggta gtagttcgtt
     2221 cgagatcccc aagagaccag caccgtcacg atcgtagaga ggtaggccga tactccgaca
     2281 accatcgcgg ctgccggcgc aagtcccact gtcggtttgg ccaagtcgct cgatgccgcc
     2341 acgatgagcc tcggcccccc gagaacaagc ccggctacga ggctaactgc gagtagcagc
     2401 agcagaagca agaagtagtc gaagcccctc cttctcagaa gttcacgact gcgtccgagg
     2461 cttcggatgg gcccggtctt ctcgatgaca ttcacgaccc cggatagccc gtagcgca
//
LOCUS       DATDOZ010000147         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2744392, whole genome shotgun sequence.
ACCESSION   DATDOZ010000147 DATDOZ010000000
VERSION     DATDOZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744392"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VI541_02830"
     CDS             complement(<1..691)
                     /locus_tag="VI541_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLF69872.1"
                     /translation="MAAGLAFPLLSMGVSPSRSPRAHLSRDLVPMISADLEKVGVIIR
                     FADEPTPDDAKALIAAGFLPGFVRYHVVPSVFAEGPMSAVRKISTNPRVRYIELNSKI
                     PYALDRATVAGRARGLWDASYRLLDQVHSDGIDGQGVGIAIVDSGIDGTHPDLIWKPM
                     AQAQTTTPKTIVNVKLVGRDSVGLSESFGFDSFMEANAQAVDVPDTDTTGGHGTHVAG
                     IASGNGSASNGR"
     gene            1118..2134
                     /gene="rsgA"
                     /locus_tag="VI541_02835"
     CDS             1118..2134
                     /gene="rsgA"
                     /locus_tag="VI541_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HLF69873.1"
                     /translation="MPARLAVENRGSYLAYTPEGEVWAEISGRFRHRVTVRSELPTVG
                     DWVALRPSDNGPALITHLFERRTKFSRKQSGDRYDIDSDRIARVEEQIIASNIDRLFV
                     VSALTDEFNSRRIERYLTMAWEGGSVPVLVLNKSDLTDPAPKLEQAASSCPGVEIITT
                     STINGDGIDGLRSYLVAGTTGALVGSSGVGKTSLINSLIQDSDRAVQPTRDDDRGRHT
                     TSRRELVRLPGGGYLIDTPGLRELQLWTTNDGLSTAFDEISELATGCRFRDCTHMHEP
                     GCVVMAALESGGLEQDRYDSYRKLQREAERLVRKQDPMLRREELRRWKAISKSMRDAK
                     PGRH"
     gene            complement(2154..2897)
                     /locus_tag="VI541_02840"
     CDS             complement(2154..2897)
                     /locus_tag="VI541_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HLF69874.1"
                     /translation="MPEEAGTTAAEMGFGWRKLQVMGAIIFLVAPLIPMIMHGFEPFI
                     MIIVVPVLIGLLLVRFARRVGVIWVGVVCLALLAMNLPFLLPSLTHPEVPVDFVPTVM
                     IALGASMAIISTIPAFGGGKGAEQRSATARALAVLTVLAIIAGAGTAFAAKGNIESQS
                     AQSGDIEVLQKEFVFHPDKIEAQGQEVALHITNSDSTRHTFTIDKLGVDVSVAPGQAV
                     RVTFTAAPGTYHFYCIPHAPDMSGDLTLT"
     gene            3030..3668
                     /locus_tag="VI541_02845"
     CDS             3030..3668
                     /locus_tag="VI541_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLF69875.1"
                     /translation="MEEEREIAKFFDCCETPGQRKMNTRISRRARAELVSGLQEIGLE
                     GRSILEIGSGPGELTRLLVQLGAARAHGIDLAEKTLEEARKRAAQEGLSDKIDYRVGN
                     GAKEALEPHDIVVLDKVICCYPDWHELVDNTSAAAKHGYGFVIPRSQGLSAFVVRAFI
                     AIVGLSLKLRKCGFSPFVHNYLQIDSHLRERGFTRKQLYLGPIWMTAVYTRP"
     gene            complement(3680..>3787)
                     /locus_tag="VI541_02850"
     CDS             complement(3680..>3787)
                     /locus_tag="VI541_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HLF69876.1"
                     /translation="RAYSPSQWSRLVSLKAKFDPSNFFRLNANIPPTDI"
BASE COUNT          813 a         1136 c         1132 g          706 t
ORIGIN      
        1 agcggccgtt gctcgcggag ccgttacccg aggcgatgcc cgcaacgtga gttccatgtc
       61 ccccggtcgt gtcggtgtcg ggaacatcaa ccgcctgcgc attggcttcc atgaaggagt
      121 cgaagccgaa tgactctgag agtccaacac tatcccggcc gacgagcttt acgttgacga
      181 tggtcttggg agttgtggtc tgtgcttgcg ccatcggctt ccagatcaga tcaggatgag
      241 tcccatcgat accggaatcg acgatggcga tgcccacgcc ttggccgtcg atcccatctg
      301 agtgaacctg atcgagaagt cggtagctcg catcccatag tcctcgggct ctaccggcaa
      361 ccgttgcacg gtcgagggcg taagggatct tcgagttgag ttcgatgtag cgaacgcgcg
      421 gattggtcga gatcttccga acggccgaca tgggtccttc ggcgaataca gatggaacga
      481 cgtgatagcg cacgaagccc ggcaggaatc ctgctgctat gagggccttt gcgtcgtccg
      541 gagttggctc gtcagcgaat cggatgatga cgccgacctt ctcgaggtcc gcgctgatca
      601 tcggaacaag gtctcgcgag agatgagcac gcggggacct cgacgggctc acgcccatag
      661 aaaggagggg gaacgcgaga ccagcggcca tcaaggccgc aagtagtctc gttctcgaaa
      721 acggtcccat caaccggcca ccattcttcg tctgccagtc gccggggaag gcccccccaa
      781 ctaaaagaga ttaacgcact aggtaataga tgcgttatgg gttgatctca aggcttggct
      841 actctctgtt gttcagatag aattaggcat ctgtccgttt cgtgaccgtt tctcgcggtc
      901 tctcgataag aaggaggtga gggatgaacc aggtcaatgt tggatctccc caccccggac
      961 gggcgcaagt caccgactga tcatctctcc ccgtccgggt acccctttcg agaccccatg
     1021 acggaggaaa gcagtgacat acccacacac cctgcccgct ctgggctggg acaagttctt
     1081 cgcctcacaa cttgaatcat tggatgaccc tttactggtg cccgcgcgcc tagccgtaga
     1141 gaaccgcggc tcctatctcg cctacacccc ggagggcgag gtttgggctg agatttcggg
     1201 gcgctttcgt catcgagtga ccgtcagaag cgaacttccg accgttggag attgggttgc
     1261 tctgcggccc tcggataacg ggccagcact catcactcac ctcttcgaac gccgaacgaa
     1321 gttctcgcgc aagcaatcag gcgaccgcta cgacatcgat tcggaccgga tcgcgcgggt
     1381 cgaagaacag atcatcgcgt ccaatatcga tcgactattc gtagtcagcg cgctgaccga
     1441 cgagttcaac tcaagaagga tcgagcgtta cctcacgatg gcgtgggagg gaggctccgt
     1501 gccggtcctg gttctgaaca agtccgacct cactgacccg gcccccaaac tcgaacaggc
     1561 tgcatcttca tgccccggcg tcgagatcat caccacgagc accatcaacg gtgacggcat
     1621 cgacggccta cgttcatacc tcgtcgccgg gacaaccggc gcattggtcg gctcgtccgg
     1681 ggtcggcaag acatcactga tcaactctct catccaggac agcgatcggg cggttcagcc
     1741 aacgcgcgat gacgaccgcg gtcggcacac tacgagccgc cgagagctag ttcgcctccc
     1801 tgggggtggg tacctcatcg acactcccgg acttcgagaa cttcagttat ggacaaccaa
     1861 cgatggactc tcgacggcct tcgacgagat cagtgaactc gccacagggt gccgcttccg
     1921 cgactgcaca catatgcacg aacccggctg tgtggtgatg gcagcccttg agagcggagg
     1981 gctcgaacaa gaccggtacg acagctatcg gaagttgcaa agggaagcgg agcgtttagt
     2041 tcgcaaacag gaccccatgc tccgtcgcga agagctacgc aggtggaaag cgatcagcaa
     2101 gtccatgcgc gacgcgaagc caggtcggca ctagaccgcg gtcccaccag ggcttacgtc
     2161 agggtcaggt ctccgctcat atcgggggca tggggtatgc agtagaagtg ataggtgccg
     2221 ggcgccgcgg tgaaggtcac cctcaccgcc tgccccggcg cgacgcttac atcgacgccg
     2281 agcttgtcga tggtgaaggt gtgacgagta ctgtcgctgt tcgtgatgtg cagtgcgacc
     2341 tcttggccct gcgcctcgat cttgtcgggg tgaaacacga actccttctg caggacctcg
     2401 atgtcacctg actgcgctga ctgactttcg atgttgccct tggctgcgaa tgcagtaccg
     2461 gccccagcga tgatggccag gaccgtaagg acggccaggg ctctggccgt tgccgaacgt
     2521 tgctcggccc ccttgccacc gccgaatgcg gggatcgtgc tgatgatcgc catcgatgcg
     2581 cctaaggcga tcatcacggt gggaacgaag tcgacgggga cctcggggtg cgtcaggctt
     2641 gggagcagga acggcaagtt catcgccagc agtgcaagac agacgacgcc gacccagata
     2701 actcccactc gccgggcgaa cctgacgagg agcagcccga tcagaacggg tacgacgatg
     2761 atcatgatga agggctcgaa accatgcatg atcatcggga tcaacggagc aacaaggaag
     2821 atgatcgcac ccatgacctg aagcttgcgc cacccgaaac ccatctcggc agcagtcgta
     2881 ccagcctctt ccggcatcct gaaacctccc ccttcggacc gaccaatcac acgcatccta
     2941 gtccgcgggc atcgggcaca agggccaaca acaatggccc gctggccagc ctgattcttg
     3001 aggccctgag agcgactaga ctccgaacca tggaggaaga acgggagatc gccaagttct
     3061 tcgactgctg cgagacaccc ggccagcgca agatgaacac gcgcatcagt cggcgtgccc
     3121 gagcagagct tgtctccggg ctccaggaga tcgggctcga aggccggtcc atactcgaga
     3181 tcggctccgg accaggggaa ctaacccggc tcctggttca actcggagca gcgcgggcac
     3241 atgggatcga tctcgccgaa aaaaccttgg aagaggcgcg caagcgcgcg gcacaggaag
     3301 gtctgtccga caagatcgac tacagagtcg gcaacggagc gaaagaggcc ctcgaacccc
     3361 acgacatcgt cgtactcgac aaggtcatct gctgctaccc ggattggcac gaactcgtcg
     3421 acaacacgtc agctgctgcg aaacacgggt acggattcgt gataccgaga tcgcaggggt
     3481 tgagtgcttt cgtcgttcgg gcattcatcg cgatcgtcgg cttgtcgttg aagctccgca
     3541 agtgcgggtt cagccctttc gtccataact acctgcagat cgactctcac cttcgagagc
     3601 gcggattcac gcgcaagcaa ctgtacctcg gccctatctg gatgaccgcc gtttacacgc
     3661 ggccctgagg cccatcacat cagatgtcag tgggtgggat gttggcgttc aggcggaaga
     3721 agttgctcgg atcgaacttg gctttgaggg atacgaggcg tgaccactga ctggggctat
     3781 atgcgcg
//
LOCUS       DATDOZ010000148         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2744510, whole genome shotgun sequence.
ACCESSION   DATDOZ010000148 DATDOZ010000000
VERSION     DATDOZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744510"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..825
                     /locus_tag="VI541_02855"
     CDS             55..825
                     /locus_tag="VI541_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69877.1"
                     /translation="MTKQQSPSERRPAKARGTIAVPRMSPKRVLTPWYRRGPSIAAMV
                     VILLGLLGLGVKVFQDIQSRSKSQKQERQTVQLFQRGLQILQSPMQDVFTSINTAPDD
                     FKAGKLSPEDFKRETLTWLEAFRRLDAGIRSRQIPSGQRALEESKVLLLQGAVVYIDA
                     VKMFGLAASQATPELRDQVIDQARATFAHATDVFGMGQRQLAGEMRRLGLERKDPTKP
                     NPLLDQPLKLPSETPPPPPPDAPPGSSGGVIPGQGQAP"
     gene            848..>2882
                     /locus_tag="VI541_02860"
     CDS             848..>2882
                     /locus_tag="VI541_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLF69878.1"
                     /translation="MRMTDDLTEAFAAGYDFTFDDFQTNACRALERSESVLVAAPTGS
                     GKTVVGEFAVWLALRSRGKVFYTTPLKALSNQKFADLCAIHGTNRVGLLTGDNSINAG
                     APVVVMTTEVLRNMIYERSDLLVGLMFVVLDEVHYLQDRYRGAVWEEVIIHLPVDVQI
                     VSLSATVSNAEEFADWIQTLRGPTAVVIEERRPIALEQHFLVEGKIVPLFVGQSSEGK
                     INPQIRRIHARKVGSDRGRGSRVQRSDVVGLMEGMEMLPAIYFIFSRKGCDSAVEQCI
                     REGVRLIDRQEAARVRRSVDERFKEFDDSDLEVLGYERWLTALTKGVAAHHAGLIPAF
                     KETVEELFLSGSIKVVFATETLSLGINMPARTVFIESLFHFTGDQHEMLTPGSFTQLT
                     GRAGRRGIDVVGHSVVPAQREVTVQQIAGLATTRTFPLRSSFEASYNMTANLVRNYSM
                     AEAEHLLNSSFGQYQADSSVVVLEKLIERNRAYMMSYREKMACDLGEFSEYLTLVEGL
                     ETVEREARELIGSQRATAVRNFVATLRPGDAFFSRGGKARGPVLVVAADRSRRGDPRI
                     LVLTPDRRLTRLTTNDLLGPASVTRLPDSTSIKDLSGSRSIPSPIRRALASQLSSLDL
                     LDQQGKAQPSLPPPETIELSRAVEDHPCHACPDRSRHMQWAQRLKRLEREESGL"
BASE COUNT          587 a          879 c          854 g          562 t
ORIGIN      
        1 ctatgaagga accatcatcg ccgtgaaact tctcaagcgt tagactgccg gcccatgacc
       61 aaacagcagt caccgagcga gcgccgtccc gcgaaagccc gcggaactat cgccgttccc
      121 cgcatgtcgc ccaagcgagt gctgacaccc tggtataggc gcgggccttc gatcgcggcg
      181 atggtagtga tcctcttggg cttgctgggc ctcggagtga aggtattcca ggacatccag
      241 agtcggtcca agagccaaaa gcaggagcgg cagaccgtcc aactcttcca aaggggcctt
      301 cagatcctcc aatcaccgat gcaggatgtc ttcacgagca tcaacactgc ccccgacgac
      361 ttcaaggccg ggaagctctc acctgaagac ttcaaacgcg agacgctcac atggctcgaa
      421 gctttccgga gactcgatgc ggggatcagg tcccgacaga tcccctctgg gcaaagggcc
      481 ctcgaggagt cgaaggtcct tcttctgcag ggagcagttg tatacatcga cgccgtgaag
      541 atgttcgggc tggcagcatc tcaggcgaca cccgagttgc gtgatcaggt catcgaccag
      601 gcccgggcca cattcgccca cgctaccgat gtcttcggga tgggtcagcg acagctcgct
      661 ggtgagatgc ggagattggg ccttgagcgc aaggatccaa ccaaaccgaa tcctcttctg
      721 gaccaaccgc tcaaacttcc ttcggagaca ccgccgccac ctccgccaga tgctccgcca
      781 gggtcgagcg gtggagtgat accgggacag ggccaagcgc cgtaacgacg tacccgccgc
      841 gtcggccatg cggatgactg acgacctcac cgaggcgttc gcagccggct acgacttcac
      901 cttcgacgac ttccagacca acgcctgccg agccctggag aggtccgaat cggttctcgt
      961 cgcggctccg acaggttccg gcaagactgt tgttggggag ttcgccgtgt ggctggcact
     1021 tcgctcgaga ggcaaggtct tctacacgac tcctctcaag gccctctcga accagaagtt
     1081 cgccgatctc tgcgcgatcc acggcaccaa cagggtcggg cttctcacgg gagacaactc
     1141 gatcaatgcg ggggccccgg tcgttgtcat gacgacggag gtcttacgca acatgatcta
     1201 cgagcgctcg gaccttctcg tgggactgat gttcgttgtg ttggacgagg tccattatct
     1261 tcaagatcgt tatcgaggag cggtatggga ggaggtgatc atccatcttc ccgtcgatgt
     1321 tcagatcgtc tcgctatcgg cgaccgtgtc caacgccgaa gagttcgccg attggataca
     1381 aacactgcgt ggccccactg ctgttgtgat cgaagagcgc aggccgatcg ccctcgagca
     1441 acacttctta gttgaaggaa agatcgtccc cctcttcgtt ggtcaatctt cggaaggaaa
     1501 gatcaacccg cagatccgtc ggatccatgc tcgcaaggtg ggatctgaca ggggtagggg
     1561 cagccgcgtg cagcggtctg acgttgtggg actgatggag gggatggaaa tgctccctgc
     1621 gatctacttc atcttctcgc gaaaggggtg tgactcagcg gtcgaacagt gtatccgtga
     1681 aggggtacgg ctgatcgatc gacaagaagc cgcgagggtg cgacggagcg tcgatgagcg
     1741 cttcaaggag ttcgacgact ccgatcttga ggtccttggt tatgagcgat ggctcaccgc
     1801 attgaccaag ggtgtcgcgg cccatcatgc cggtctgatc ccggccttca aggagaccgt
     1861 cgaggaactc ttcctctcgg gctcgatcaa ggtcgtcttc gcaactgaga cgctctcgct
     1921 cgggatcaac atgccagcgc gcaccgtgtt catcgagagt ctcttccact tcacgggaga
     1981 tcaacacgag atgctcactc cggggtcgtt cacacagttg acgggtcgtg ccggaaggcg
     2041 cggtatcgac gtcgtcggtc attcggtggt acccgctcag agagaggtca cggttcagca
     2101 gatcgccggc ctcgctacca cacggacctt cccgctgagg tcttccttcg aggcttcgta
     2161 caacatgacc gccaacctag tgcgaaacta ctcgatggct gaggcggagc atcttctgaa
     2221 ctcttctttc gggcagtatc aggccgacag ctctgttgtg gtcctggaga aactgataga
     2281 gagaaaccgc gcatacatga tgtcttaccg cgagaagatg gcttgcgacc tgggagagtt
     2341 ctccgagtac ttgacgctgg ttgaggggct cgagactgtc gagcgagagg cgcgggaact
     2401 catcggtagt cagcgagcca ctgccgtccg caacttcgtc gcgacgctcc gtcctgggga
     2461 cgcattcttc tcacgaggcg gcaaagcgcg cggtcctgtt cttgtggtgg ctgccgatcg
     2521 ttcgcgacga ggcgacccac gcatccttgt gctgaccccc gaccgacgcc taacgcgcct
     2581 cacaaccaac gacctgctcg ggccagccag cgtgaccaga ctgcccgact cgacatcgat
     2641 caaggacctg tccggttcca ggtccatccc atctccgatc agacgagcgt tagcttcgca
     2701 gctctcatcc cttgatctac tcgatcagca gggcaaggcc cagccgagcc tgccgccccc
     2761 ggagaccatc gaactcagta gggccgtgga agatcacccg tgtcacgcat gtccggaccg
     2821 ctcccgacac atgcagtggg cccagcgact caagcgtctc gagagagagg agtcggggct
     2881 gc
//
LOCUS       DATDOZ010000149         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_905353, whole genome shotgun sequence.
ACCESSION   DATDOZ010000149 DATDOZ010000000
VERSION     DATDOZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905353"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VI541_02865"
     CDS             <1..746
                     /locus_tag="VI541_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLF69879.1"
                     /translation="LIYSGAITTWSDPILMIGDPTTASDDNPGLAGCGLGIDVAKRED
                     IATSTNVLKEYLAIANPMFNGLKAKELTQVWPPTLPTGCRAIGDAGMSTCLATPGTLG
                     HVLYQEAYSKTNYKMAELQNIGGGFAAPATNVSATPARTTYPDNCYSAAAASASSLPP
                     TQIDWSRVTLTNTTSGYPLCQFSYLLGYSRPEAAMAGVGPGQVRTMVDYLTDIARDDV
                     QEGLKTRQVSPLPPAIRTLLRIGITNISP"
     gene            782..1474
                     /locus_tag="VI541_02870"
     CDS             782..1474
                     /locus_tag="VI541_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HLF69880.1"
                     /translation="MAIIDINADMGEGAPNERELLAHVTSASIACGGHAGDAASMSIS
                     IAACIEHGVRVGAHPSYPDRSNFGRRSIAMAPDELNKVVQEQIAALREVALEHGCAVS
                     YFKPHGALYNDAASLESIAESLLQVSLQLDLPIMLLAGSALESLASDLGTEYIREGFI
                     DRRYHADGSLVARSDDDALITDPSDAAAHAIVLAQMVDSLCIHSDTPGAPNIAAVARM
                     ELERAGHSIGVR"
     gene            1471..2055
                     /locus_tag="VI541_02875"
     CDS             1471..2055
                     /locus_tag="VI541_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HLF69881.1"
                     /translation="MRVQRFGDRGLRIVFDEEPSKELTLKLRSLCKEARSLEGVIDAA
                     PGQVSAALLLDGDPDLVERALGKLSVEPEPLASASHIVQVIYDGVDLEWIAHNKSITV
                     DDIVALHAGREYFVRMLGSPGFIYLSDVHPTLQVERLATPRPQVPAGSIGVAGLQTGI
                     YGRGRPGGWRIVGKANEVPEVAPGDTVRFAAVTQ"
     gene            2052..2831
                     /locus_tag="VI541_02880"
     CDS             2052..2831
                     /locus_tag="VI541_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HLF69882.1"
                     /translation="MISILRAAVATSIQDRGRYGHLAEGITISGAMDVASLDATNALA
                     GAMPGSAGIEFGPGPLRIEVHQGGTIAFGGAPRRGAPWWQTLAVRPGQTFELSSPQSG
                     VWSYLCVEGGVSAPLHMGSRSTTVREGIGSWLTAGSPVLAGHERAEPSVPQPPPMRGE
                     IRMFGELLGAFIVGEKVDRMGYMLRGGSFAPYEAVDRSEPLLPGCVQVTPSGDAIALM
                     AEAPTVGGYRVEGVIHSADLRLLAQTPALHPIYFRNLSWMG"
     gene            2836..>3620
                     /locus_tag="VI541_02885"
     CDS             2836..>3620
                     /locus_tag="VI541_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLF69883.1"
                     /translation="MTVMQSTPARTRTYGAFFLSSAIWGSTFLVIRLGNNAAVPPVWA
                     ATLRLGLATALLIGLTYLMRQRMPRGAALRAAAWYGFFEFGVNFPLLYWAETTVPSGL
                     TSVFYATIPITTPLLAWMIGQETLSLRKILGALAALVGVVIIFGGEIGTGTNIWPLLA
                     LVVSSTTAGFAGVMLRRAPPQAAIPANAVGAAVGFVICLLASFAMGERHAIPTRLPQW
                     GPIVYLAIMGSMGAFLLWSWLLKHWSATAANFVSVIVPVIAVLL"
BASE COUNT          674 a         1144 c         1116 g          686 t
ORIGIN      
        1 cgctgatcta ctctggcgcg atcactacat ggagcgaccc gatcctgatg atcggcgacc
       61 cgaccaccgc cagcgatgac aacccgggtc tcgccggatg cggacttggg atcgacgtag
      121 ccaagcgtga ggacatcgcg acctctacga atgtgctgaa ggagtacctc gcaattgcaa
      181 acccgatgtt caacgggttg aaggctaagg agctgactca ggtttggccg ccgaccctgc
      241 cgactgggtg cagagcgatc ggtgacgctg gcatgtcgac ctgcctcgca actccaggga
      301 cgctcggcca tgtcctctac caagaggctt acagcaagac caactacaag atggcagagt
      361 tgcagaacat cggcggcggg ttcgctgcac ccgccaccaa cgtgagcgca acacctgctc
      421 ggacgaccta tccagataac tgctactcgg ccgcagcagc ctcagccagc agcctgccac
      481 ccactcagat cgattggagc agagtgactc tcaccaatac gacaagcggc tatcccctct
      541 gtcaattctc ataccttctc gggtattcga ggcctgaagc cgccatggcg ggagtcggac
      601 cgggacaggt tcgcacgatg gtggactatc taaccgacat cgcccgagat gacgtccagg
      661 aagggttgaa aacccgccag gtgtcgccgc tcccgcctgc catcagaacc ctcctgcgga
      721 tcggcatcac gaatatcagc ccgtagtagt accgcgacga aagaggctgt agcctcgcag
      781 cgtggcgatc atcgacatca acgctgatat gggggagggg gcccccaatg agcgcgaact
      841 tcttgcccat gtgacgtcag cgagcatcgc gtgcggaggt cacgccggcg atgctgcctc
      901 gatgagcatc tccatcgccg cgtgcatcga gcatggcgtg cgagtggggg cccacccctc
      961 ttaccccgat cgcagcaact tcgggcgccg cagtatcgcc atggcccccg acgaattgaa
     1021 caaggtcgtt caagaacaga tcgcggcact ccgggaagta gcgctagagc atggctgcgc
     1081 ggtctcgtac ttcaaaccac atggcgccct atacaacgac gctgcctcgc tcgagtcgat
     1141 cgctgagtcc ctcctgcagg tatcacttca actcgacctg cccatcatgc tccttgcggg
     1201 ctcggccctc gagagcctgg cctctgacct gggcaccgaa tacatcagag aggggttcat
     1261 cgaccggcgc taccacgccg atggttcgct ggtcgcgcgc tccgatgatg acgcactgat
     1321 cacagaccca tccgacgccg cagcacatgc gatcgtgctt gctcagatgg tcgactctct
     1381 gtgcatccac tcggacactc cgggtgcccc caacatcgca gcagttgctc gcatggaact
     1441 cgagagggcc ggacactcga tcggcgtgcg gtgagggttc agcggttcgg cgaccgaggg
     1501 ctccgtatcg tattcgacga ggagccctcg aaagagttga cactcaagct gcgctctctc
     1561 tgcaaggaag cccgaagttt ggagggtgtg atcgatgccg ctccgggtca agtgagcgct
     1621 gcacttctgc tcgacggtga tcccgacttg gtcgagcgcg ccttgggcaa actctcggtc
     1681 gaaccggaac ctctcgcgtc tgcatcgcac atagttcaag tcatctacga cggggtagat
     1741 ctggagtgga tcgctcataa caagtcgata acggtcgacg acatcgtcgc actccatgcg
     1801 ggcagggagt acttcgtgcg gatgctcggc tcccccggct tcatctacct ctccgatgtt
     1861 catccaaccc ttcaggtcga gcgattggcg acgccgcgcc cgcaggtgcc cgctggttcg
     1921 atcggagtgg ccggtctaca gaccggcatc tacggccggg gccggcccgg aggctggcga
     1981 atagtcggaa aggcaaacga ggtcccagag gtcgcacccg gcgacacggt gcgattcgcc
     2041 gcggtgacac agtgatctcg atcctgcggg cagcggtcgc aacgagcatc caggatcgcg
     2101 gacgctacgg tcacctcgca gaaggcataa ccatctcagg ggccatggat gttgcatctc
     2161 tcgacgctac caacgcgctt gcgggggcta tgcctggcag cgccggtata gagttcggac
     2221 cgggcccctt aaggatcgag gtgcaccagg gcggcaccat cgcgttcggc ggggctccgc
     2281 gacgtggggc accctggtgg cagaccctcg cggtgaggcc gggtcagacc tttgagctgt
     2341 cctcaccgca gagcggggtc tggtcatacc tctgcgttga aggaggcgtc agcgctcccc
     2401 tccacatggg aagcagatcg accacggtgc gagaggggat cgggtcgtgg ctgacagcgg
     2461 gctccccagt gctcgcaggg cacgagaggg ccgagccctc ggtgccgcag cccccaccga
     2521 tgcgcggtga gataaggatg ttcggtgagt tgctgggagc cttcatcgtt ggagagaagg
     2581 ttgaccggat gggatacatg ctgcgaggtg gatcattcgc gccatacgag gcggtagatc
     2641 ggtccgaacc cctccttcct ggatgcgttc aggtaacacc atctggcgat gccatcgcat
     2701 tgatggccga ggctcccacg gttggtggat atcgagtcga gggcgtcatc cactcggcgg
     2761 accttcgcct gctggctcag acaccagccc tgcaccccat ctacttccgc aacctgtcat
     2821 ggatggggtg agaggatgac cgtcatgcag tcaacgcctg cgcggacgag aacctacggc
     2881 gcgttcttcc tatcaagcgc gatctggggt tcgacattcc tggtcatccg cctgggcaac
     2941 aacgcggcgg tgcctcccgt gtgggccgcg acccttcggc tgggactcgc tactgccctt
     3001 ctgatcgggc tgacatacct gatgcgccag aggatgccgc gtggcgcagc gctgcgggcg
     3061 gcggcctggt atggattctt cgaattcggg gtcaacttcc cgctgctcta ctgggccgaa
     3121 acaaccgtcc cgtcggggct aacctctgtc ttctacgcaa cgatccctat caccacgccg
     3181 ctgctcgctt ggatgatcgg ccaagagaca ctgagccttc gcaagatcct cggcgcgttg
     3241 gctgccttgg tcggcgtcgt gatcatcttc ggaggagaga tcggtaccgg taccaacatc
     3301 tggcccctcc tagcacttgt tgtctcatcg accacggcgg ggttcgctgg ggtgatgctc
     3361 cgcagagcgc caccgcaagc agcgatccca gcgaacgccg tgggcgctgc tgttgggttc
     3421 gtcatctgct tgctcgcttc gttcgcgatg ggtgagcggc acgcgatccc aacgcggctg
     3481 ccgcagtggg gaccgatcgt ttatctggcc atcatggggt cgatgggtgc attcttactc
     3541 tggtcgtggc tgctcaaaca ctggtccgcc acggccgcca acttcgtctc ggtcatcgtg
     3601 ccggtgatcg cggtgctgct
//
LOCUS       DATDOZ010000150         5001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_644971, whole genome shotgun sequence.
ACCESSION   DATDOZ010000150 DATDOZ010000000
VERSION     DATDOZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644971"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VI541_02890"
     CDS             <1..359
                     /locus_tag="VI541_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69884.1"
                     /translation="DGSALDALTETGAQALAAGGLAYVMNPSLKTDREDEDEETASEP
                     STQSTVDVGDVIDTSGAVVESILEPMSEHARTAEQVGSYTADLQKEMPKDASSEETSS
                     SPEPPASPSEEPSEEN"
     gene            complement(383..1873)
                     /locus_tag="VI541_02895"
     CDS             complement(383..1873)
                     /locus_tag="VI541_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HLF69885.1"
                     /translation="MRHARRSAFPWSRTDTLALTLITIFGGLLRFVRLASPKGFVFDE
                     TYYAKDACLYLGLGQSYCRLDQATEQSYVHPPLGKWLIAIGERLFGYNEFGWRVASAV
                     VGTALIVVIYLLARKLFHHRWIAGAAALLTATDFLLLVQSRIAMLDIFQSFFVALGFL
                     FLAYDRADVIRWRSEALLPFPAPPRVRELEWRFAAGASFGLALGVKWSAAWALIGAAS
                     LAAIWSISLIRAGSRLPGPKKRGLWEALALVIALGVIPVAAYLSSYTKYFVDTASVEC
                     PYRGPKESEYGRKPVDRSKPATDLGRINFGVQTDQCKSGPYGVALAFIDLQGRMANYH
                     LTLNATHAYKSSAASWPIVRRPVAYYFESKPEDSQILAFGNPAVWWAALAGALLLLSR
                     SLRPRAFQPERVVAVAWASQYLPWLAVTRPLFFFYMTPVAPFMMIGLAVALEKLRSLG
                     RGPRWLVTTYFAIGVMALLWYFYPVIAAVGLRPDLWRSRMWFRSWI"
     gene            1944..2282
                     /locus_tag="VI541_02900"
     CDS             1944..2282
                     /locus_tag="VI541_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HLF69886.1"
                     /translation="MQFAVTTDRDILIEVEVPRGSLDRMKGLLGRDLLAPGNGMLLKG
                     KQVHTFGMRFAIDALYLDGDHRVLSVHRLAPRRLGPLLLRARSVLELADGEASRLGIE
                     PGVTLKVVKE"
     gene            2279..3121
                     /gene="rsmI"
                     /locus_tag="VI541_02905"
     CDS             2279..3121
                     /gene="rsmI"
                     /locus_tag="VI541_02905"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLF69887.1"
                     /translation="MSGTLFICATPIGNLEDVSRRLVRVLSEVDLVAAEDTRRTRKLL
                     SAIGVSARLVSYHDVNERKQTPFLIRTLESGRDVALVSDGGTPAISDPGYALVRAAID
                     KGFDITVIPGPSSVTTALVLSGLPTARFAFEGFLPRGASEQRTRLRELAAEQRTLVFF
                     EAPTRVKGSLVAILEVFGDRRVSVARELTKIHEEVLRGPLSEVITQLEGRDILGEVVL
                     VIEGASEVGKLEDAVERARALVIAGSSPSQAAALSAKEFHVHRRDVYGSLVSDQVAKG
                     SQSG"
     gene            complement(3091..3363)
                     /locus_tag="VI541_02910"
     CDS             complement(3091..3363)
                     /locus_tag="VI541_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLF69888.1"
                     /translation="MKDTGMHRKVDDLGRIVLPAEMRKSFGIKEGDLLDISVDEDRII
                     LSLATDSCIFCRSKNDLKEFREKTVCAACIRDLTNPTQPDWDPFAT"
     gene            3419..4963
                     /gene="metG"
                     /locus_tag="VI541_02915"
     CDS             3419..4963
                     /gene="metG"
                     /locus_tag="VI541_02915"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HLF69889.1"
                     /translation="MSKDVFYITTPIYYLNDVPHIGHAYTTVAADFVARYHRLAGEKV
                     FFLTGTDEHGEKVARAAEAKGLGPQEWCDQLVLRWHEVWDRLAISHDDFIRTTEPRHM
                     GPVQALVQKVYDKGDIYLGEYEGPYCVSCEAFYQPSEIVDGLCPIHEKPVEMIKEENY
                     FFRLSAYQDRLLALYEEQPDWVRPDVRRNEVIGFVKGGLADLSISRTSFSWGVPIPWD
                     PKHVMYVWIDALQNYTTVVGQGTDEARFASSWPADYHFIGKDILRQHAVIWPAFLMAA
                     GIELPRCVFAHGYLTVGGKKMSKTNLTGISPHTLVDTFGVDGYRYHFLREGTFGQDGA
                     FSWEAMLARYNADLANDLGNLVSRSLAMVVSYRNGVVPGAGEITSAEDALRISAQSAR
                     EELDRGVRALDLGGALAGIFRFVADVNHYIDAAAPWKLAKDPDQSARLDSVLNSICEA
                     IRHLSILISPAMPGAAERIREQLGVSATDDRPLDEALAWGGLLEGTKVSRGESIFPRV
                     EADTQD"
BASE COUNT         1012 a         1482 c         1554 g          953 t
ORIGIN      
        1 atgacgggtc ggccctcgat gccctgacgg agacgggcgc acaggcactg gcggctggtg
       61 ggctcgccta cgttatgaac ccttctctca agaccgaccg cgaggatgag gacgaggaaa
      121 cggcatccga accttccacg cagtcgacgg ttgacgttgg cgacgtcatc gatacgagcg
      181 gggctgtcgt cgagagcatc cttgagccga tgtcggaaca cgcacggacg gccgagcagg
      241 tcggaagcta caccgctgac cttcagaagg agatgccaaa ggatgcttcg tcggaagaga
      301 catcctcttc acccgagccc ccagcgtcac catcggaaga accctcggaa gagaactaga
      361 tctcggtagc ccagctgagt cttcagatcc agctgcggaa ccacatgcga cttcgccaca
      421 ggtcgggcct caagcccacg gctgcgatca cggggtagaa gtaccagagt aaggccatga
      481 ccccgatggc gaagtaggtc gtgaccagcc atctcggccc ccgacccagc gatcgcaact
      541 tctcgagggc caccgcgagg ccgatcatca tgaacggcgc gacgggggtc atgtagaaga
      601 agaagagggg ccgcgtgacc gctaaccacg ggaggtattg cgaggcccac gctaccgcga
      661 cgaccctctc cggctgaaat gccctcgggc gcaggctccg agatagaagc aggagagccc
      721 ccgcaagcgc ggcccaccag accgccggat tcccgaacgc gaggatctgc gaatcttctg
      781 gtttgctctc gaagtagtac gcgacgggtc tgcggacgat cggccaactc gccgcggatg
      841 atttgtaagc gtgggtcgca ttcaatgtga ggtgatagtt cgccatcctg ccctgcaggt
      901 cgatgaacgc cagcgcgacg ccgtagggcc ccgacttgca ctggtcagtc tggacgccga
      961 agttgatccg gcccagatcc gtcgcaggct tcgaacggtc gactggcttg cggccgtact
     1021 cagactcttt cggcccccga tatgggcact caaccgatgc ggtgtcgacg aaatacttgg
     1081 tgtatgagga tagataagcc gcaacgggga tgaccccaag tgcgatcacc agagcgagag
     1141 cttcccagag ccctctcttc tttgggccag gcagtcgtga cccagctctt atcaatgaga
     1201 tcgaccagat cgctgcgagg gaggcggctc cgatcagcgc ccatgcggca gaccacttca
     1261 ccccaagagc gagtccgaac gacgccccag ctgcgaagcg ccactcgagt tccctcacgc
     1321 gagggggggc cgggaagggc aacaacgcct cgctcctcca tcggatcacg tctgctcggt
     1381 cgtatgcgag gaagagaaag ccgagtgcca cgaagaacga ttggaagatg tcgagcatcg
     1441 cgatgcgact ctgtacgagc agaaggaagt ccgttgccgt cagcagagcc gcggcccccg
     1501 cgatccatcg atgatgaaac agtttgcggg ccagcagata gatgaccacg atgagcgctg
     1561 tcccaacaac cgcggatgcc accctccagc cgaactcgtt gtatccgaag agtcgctcgc
     1621 cgatggcgat gagccacttc ccgagcggcg ggtgaacgta gctctgctcc gtcgcttgat
     1681 cgagcctgca ataggactgt ccgagtccga gatagagaca ggcgtccttg gcgtagtagg
     1741 tctcgtcgaa tacgaatcct ttcggcgagg ccagtctcac gaaccggagc aggcccccga
     1801 agatcgttat gagggtcagc gcgagcgtgt cggttcggga ccaggggaaa gcgctgcgac
     1861 gtgcgtggcg cactcgattt ctccatccgg gaggcaaact cggtcagtat agcgacgcgt
     1921 tcgcggctca aaggggagca tctttgcaat tcgctgtgac gacggatcga gacatcctca
     1981 tcgaggtcga ggtgcccagg ggctcgctgg atcgcatgaa gggtctcctg ggtcgagatc
     2041 ttctggcacc tgggaatggc atgcttctga aaggtaagca ggtacatacc tttgggatga
     2101 gattcgccat cgatgctctc tacctcgacg gcgaccatcg cgttctatcc gttcatcgtc
     2161 ttgcgcccag acgtctcggt ccgctcttgt tgcgggcgcg ctcggttctc gaacttgcag
     2221 atggtgaagc gagccgcctc gggatcgagc caggtgtgac cttgaaggtc gtcaaagagt
     2281 gagcggcacc cttttcatat gtgccacccc tatcggcaac ctcgaagatg tgtcgcgtcg
     2341 tctcgtacgg gtgctatccg aagtcgatct cgtcgctgcc gaggacacgc gccggacgcg
     2401 caagcttctg agcgcgatcg gtgtcagtgc ccggctcgtt tcgtaccacg atgtcaacga
     2461 acgcaagcag acaccgttcc tgatacggac actcgaatcg ggtcgtgatg tggctctcgt
     2521 cagcgacggg ggcacgccgg cgatctcgga ccccggctac gctctggtgc gggcggcgat
     2581 cgacaaaggg ttcgacatca ccgtcatccc cggaccttca tcggtcacga cggcactggt
     2641 gctgtccggc ctgccgaccg cgcggttcgc attcgaggga ttcctgccgc ggggggcctc
     2701 ggagcaacgg accagattgc gtgagttagc ggcagagcag cgcacgctcg tcttcttcga
     2761 ggcccccacg cgggtcaagg gatcgctcgt cgctatcctc gaagtcttcg gagatcgccg
     2821 ggtgtcggtt gcccgcgaac tcaccaagat ccacgaggag gttctgcgag ggcctctttc
     2881 cgaagtgatc acacaactcg aaggtaggga catcctcggc gaggtggtgc tcgtcatcga
     2941 gggtgcctcc gaggtaggca agctcgagga tgcggtcgag cgcgcccgcg cgctcgtcat
     3001 agcgggttca tcgccgagcc aggccgccgc gctctccgcg aaggagttcc atgttcacag
     3061 acgggatgtc tacggatcgc tagtgagcga tcaggtagcg aaggggtccc agtcgggctg
     3121 agttgggttg gtcaagtcgc ggatgcaggc cgcgcagacc gtcttctcac ggaactcctt
     3181 gaggtcgttc ttgctccgac agaatatgca tgagtcggta gcgagcgaga ggatgatgcg
     3241 atcctcgtcg accgatatgt cgagcaggtc tccctctttg atgccgaaac tcttgcgcat
     3301 ctcggcagga agtacgatcc ggccaagatc gtcgaccttt cggtgcatac cggtgtcttt
     3361 catccagggg ataatatcct tcatttccca gcaagccaaa gtccacggga gccgttgagt
     3421 gagcaaagat gtgttctaca taacgacacc catctactac ctgaatgatg tcccgcacat
     3481 cgggcatgcc tacacgaccg tcgcggcgga cttcgtcgcg agatatcacc gccttgcagg
     3541 cgagaaggtc ttcttcctca ccggcaccga tgaacatggc gagaaggtgg ctcgggccgc
     3601 cgaggccaag ggactcggtc cgcaggagtg gtgtgatcag ttggttctgc ggtggcatga
     3661 ggtctgggat cgcttggcta tctcgcacga tgacttcatc cgtacgaccg agccgcggca
     3721 catgggaccg gttcaagccc tcgtgcagaa ggtctacgac aagggagaca tctacctcgg
     3781 cgagtacgag gggccttact gcgtttcatg cgaggccttc taccaaccga gcgagatcgt
     3841 cgatgggctc tgtccgatcc atgagaaacc tgtcgagatg atcaaggagg agaactactt
     3901 cttccgactc tcggcatatc aagaccgcct ccttgccctc tacgaggagc agccggactg
     3961 ggttagacca gacgtcaggc gaaatgaggt gatcgggttc gtgaagggcg gtcttgccga
     4021 cctctcgatc tcgcggacat cgttctcatg gggggtcccg ataccctggg accccaagca
     4081 tgtgatgtac gtctggatcg atgcccttca gaactacacg acggtcgtcg gacagggcac
     4141 cgatgaggcg cgtttcgcct cgtcgtggcc cgccgattac cacttcatcg gtaaagacat
     4201 cctccgacag cacgcagtga tctggccagc gtttctgatg gctgctggga tcgaacttcc
     4261 cagatgcgtg ttcgctcatg gatacctgac cgtcggtggc aagaagatgt caaagacaaa
     4321 cttgaccggg atctctcctc atacgctcgt cgatacattc ggggttgatg gttaccggta
     4381 tcacttcctc cgcgagggaa cattcggaca ggacggcgcg ttctcgtggg aggcgatgtt
     4441 ggcgcgctac aacgccgacc tcgccaacga tcttggaaac ctggtgagcc gctcacttgc
     4501 gatggtagtg agctacagaa atggagttgt gccaggtgcg ggagagatca cttcagcgga
     4561 agacgcgttg cggatatctg ctcagtcggc ccgcgaagag ttagataggg gcgtgcgagc
     4621 actcgacctc ggcggcgcgc tcgcgggcat cttccggttc gtcgccgacg tgaaccacta
     4681 catcgatgcg gcggccccat ggaaactcgc caaggacccc gaccagtcgg caaggttgga
     4741 ctcggtgctt aactcgatct gcgaggcgat ccgtcatctc tcgatactga tatcacctgc
     4801 catgcctggc gcggccgagc gcatccgtga acagttggga gtttctgcga cggacgatcg
     4861 acctctcgat gaggctctcg cgtggggggg ccttctcgaa gggacaaagg tctcaagggg
     4921 cgagtccatc ttcccccgcg tcgaagccga tacgcaagat tgaactggtt cgatagccac
     4981 tgtcacctcg acgcgctcga g
//
LOCUS       DATDOZ010000151         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3515841, whole genome shotgun sequence.
ACCESSION   DATDOZ010000151 DATDOZ010000000
VERSION     DATDOZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3515841"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VI541_02920"
     CDS             complement(<1..288)
                     /locus_tag="VI541_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLF69890.1"
                     /translation="MNVTTERRLGWVVVTPSGEIDLDTIDDFARVLIEVTLDQPEALA
                     VDMSDVSFWDTTALRVLIETNQQQMKHDGDFVLVVPPGPFADVVEEAKLGDD"
     gene            complement(479..838)
                     /locus_tag="VI541_02925"
     CDS             complement(479..838)
                     /locus_tag="VI541_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HLF69891.1"
                     /translation="MNTDLWTYREMVPQSDKGIAGFAVEAIDGSIGYIDETSNQAGEA
                     YIVVDTGPWIFGRRVVIPVGAIDNVDLIDERVYVEMSKEQVKNAPELKADTQVDHAFR
                     GELTDYYEPIFHGRMEM"
     gene            1005..>2107
                     /locus_tag="VI541_02930"
     CDS             1005..>2107
                     /locus_tag="VI541_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HLF69892.1"
                     /translation="MARGEPAVMAVERSDISIAPDSRRVVARPFIPGFVGTFNALGGA
                     RVDQIAERVMKLSPGEVARLLDETRRLFHHRHRDLEQRWEEHFITALPHSERMQSASR
                     DMRKLLGAVFTHEYSFEGAAICNPSLVEHPEGSRPGELAFIMSARAIGEGHISSIEFR
                     TGHIDKKGAVIIDPVFPVVEVGRRTSVIYGKRGFMAKMAELGGDPGITSQVCDPLPAR
                     FSLEDLEQSMPHLEMQMEYAADAFETVRLIHWLASSNYRLEFGESEISERLIFPAGPA
                     DSNGMEDARFVKFVDDDEQSTYYATYTAYDGFQILPQLIQTSDFRTFEIMTMDGAGAK
                     NKGMALFPSKIEGRYAALSRHDRESLYLMYSDD"
BASE COUNT          459 a          604 c          583 g          461 t
ORIGIN      
        1 gtcgtcgccg agcttggcct cttcgaccac atctgcaaac ggaccaggcg gaaccacgag
       61 cacaaaatcg ccgtcatgct tcatctgttg ctgattggtc tctatcaata cccgcagtgc
      121 agtggtatcc cagaacgaca cgtcggacat gtcgactgca agagcttcgg gctgatcgag
      181 agttacctca atcagcacgc gagcaaaatc atcgatggtg tcgagatcga tctcaccgct
      241 cggcgtgact accacccatc ccaagcggcg ttctgtggtg acgttcaaga gcagcgttct
      301 gtggtgacgt tcaaggcatc gcgctaaatc gaaggccgat tgagcgtatc actctgaaag
      361 tagctgaacg gtcgttcaaa ttcgatctcc gggatggatc taacgaatgt tttttctcat
      421 ttgaacgaaa ttcagcctgc agctcaacta tccgaccagc accgactgat acgtgatgtc
      481 acatctccat gcgaccgtgg aaaatgggct cgtagtagtc ggtcagttcg cctcgaaagg
      541 cgtgatcgac ctgggtgtcg gctttgagtt cgggcgcgtt cttcacctgc tcctttgaca
      601 tttcgacgta cacccgctcg tcaatcagat cgacgttgtc gatagcaccg accggaatca
      661 caacccttct gccgaagatc cagggtcctg tgtcaaccac gatgtatgcc tcgccggcct
      721 ggttggaagt ctcatcgatg tagccgatgc ttccatcgat ggcttcgacc gcaaacccgg
      781 caatgccctt gtcggattgg ggcaccatct ctctataggt ccacaagtct gtgttcatga
      841 caccctcctt acgagagtcg tccgagattt acccggtcgc aaccgggttg aaaccggatg
      901 ggaccagatg ccatgtgaac tgctcgggcc aggtcgatcg acctagttcc cattccatcg
      961 aacagaggta gggcacaatg aggggcgacc cgagaggaga aacggtggcg aggggagaac
     1021 cagcggttat ggcggtagag cgctccgaca tttccatagc tcccgattca cggcgagtcg
     1081 tggcccggcc cttcatcccc gggtttgtcg gcaccttcaa tgccctcggt ggtgcaaggg
     1141 tcgatcagat tgccgagaga gtcatgaagc tttcgcctgg ggaggtagcc cgactcctgg
     1201 acgaaacccg tcgtctattt catcatcgcc atcgcgatct ggagcagagg tgggaagagc
     1261 acttcataac cgctttgccc cacagcgagc ggatgcaatc ggcttctcgt gacatgcgca
     1321 agctgcttgg ggctgtattc actcatgagt actccttcga aggagcggca atttgcaacc
     1381 cttccctggt cgaacaccct gagggatcaa gacccggcga gcttgcgttc ataatgagtg
     1441 cccgggctat cggagagggc cacatctctt cgatcgagtt ccgaacagga cacattgaca
     1501 agaagggcgc agtgatcatc gatcccgttt tccctgtggt ggaggttggg cggagaacct
     1561 ctgtgatcta cggcaagcgg gggttcatgg ccaagatggc tgagttaggg ggcgatccgg
     1621 ggatcaccag ccaggtgtgc gacccgttgc ctgccaggtt cagcctcgaa gatctcgaac
     1681 agtccatgcc tcatctcgag atgcaaatgg agtatgcggc cgacgcattc gaaaccgtcc
     1741 gcctaatcca ctggctggcg tcgtccaact atcgccttga gttcggagaa agcgagatca
     1801 gtgagcgtct catcttccct gccgggccgg ctgacagcaa tgggatggaa gacgcccgct
     1861 tcgtcaaatt cgtcgacgac gatgagcaat ccacctatta cgccacctat accgcctacg
     1921 acggtttcca gatccttccc caactgatcc agacttctga cttccggacc ttcgagatca
     1981 tgacaatgga cggggccggt gccaagaaca aaggcatggc gttgtttccc tcgaagatcg
     2041 aaggtcggta cgccgcgctc tcgcgccacg accgggagag cctttacctc atgtattccg
     2101 atgacat
//
LOCUS       DATDOZ010000152         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_125891, whole genome shotgun sequence.
ACCESSION   DATDOZ010000152 DATDOZ010000000
VERSION     DATDOZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125891"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VI541_02935"
     CDS             <1..288
                     /locus_tag="VI541_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69893.1"
                     /translation="PTTAESIMAKLKEAGLPAAVPTDTHQISAGGCLLIAADQGWARV
                     VGRDVRGIRLIGLARAAPSETMYEITPAGAQALEELSNIWDQAGSAMQVIS"
     gene            509..880
                     /gene="rpsL"
                     /locus_tag="VI541_02940"
     CDS             509..880
                     /gene="rpsL"
                     /locus_tag="VI541_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HLF69894.1"
                     /translation="MPTIQQLVRKGRHTPSSKTKTPALKGSPQRRGVCTQVKTTTPKK
                     PNSALRKIARVRLTSGVEVTAYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYKIIRGTL
                     DTAGVRDRKQARSRYGAKRAS"
     gene            880..1350
                     /gene="rpsG"
                     /locus_tag="VI541_02945"
     CDS             880..1350
                     /gene="rpsG"
                     /locus_tag="VI541_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLF69895.1"
                     /translation="MPRKGPATKREAVSDPVYQSVLVTQFVNRIMLGGKKTLAEKVVY
                     GALKTVSEKTGGDPTAVLRKAVENVRPLVEVRSRRVGGSTYQVPVEVPSRRSLTLAIR
                     WMVNFSRARKERTMADRLAAELMDASNGAGASVKRREDLHKMAEANKAFAHYRW"
     gene            1568..>2787
                     /locus_tag="VI541_02950"
     CDS             1568..>2787
                     /locus_tag="VI541_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLF69896.1"
                     /translation="MAHIDAGKTTTTERILYYTGRTYKMGEVHEGGAVMDWMIQEQER
                     GITITSAATTTKWKDHWINIIDTPGHVDFTVEVERSLRVLDGAIAVFDAVAGVEPQTE
                     TVWRQADRYSVPRICFVNKMDRLGADFFMTVSMIRERLGGHPLPVQIPWGSESDFHGV
                     IDLVEMKGRYWTTEKGEEWEDREVPAELLDEANHWRHELYEALAEYDEEVMDAYVHGG
                     DPTPEHLRLALRKATLSGTAQPVLCGSAFKNKGVQPLLDAVVAYLPSPLDVKPVVGEN
                     PRTGAEEIRRPDDGEPFAALAFKIMSDPHVGRITYMRVYSGSLKSGGHLLNSTRDRKE
                     RAGRLLQMHANHREDKESVLTGDIVAIVGLKNTITGDTLCDPDHPIRLEAPIFAQPVI
                     SLAIEPKTKADQDKL"
BASE COUNT          593 a          852 c          855 g          487 t
ORIGIN      
        1 cccacgacag cagagtcgat aatggcgaag ctgaaagaag cagggttacc cgcagccgtg
       61 ccaacggata ctcaccagat ctcagctggg ggttgcctgt tgattgcggc tgatcaaggg
      121 tgggcccggg tcgtggggcg agacgtaagg ggcattcggc tcatcggatt ggcgagagct
      181 gccccaagcg aaaccatgta cgagatcacg cccgccggtg cacaggccct cgaggagctt
      241 tccaacatct gggaccaagc cggttcagcc atgcaggtga tttcctagac tcagagggtt
      301 tgaccgggtc gggggtgggt gataacttat cccttcgtgc gtgaagcgct ccttgcttcg
      361 cgcacgccgg agtgcacctg atgagcaaga tgagtcgcct gccataaagg cggcccccag
      421 atttcttgtc cgctgatcaa gctctcggag atcttgcttc gagttgcgcg tgaggctcac
      481 aacgggaatc gggaagaaag ggaaagcagt gccgacgatc cagcagctcg tccgtaaggg
      541 acgtcatacg ccgagcagca agacgaagac ccccgcattg aaggggagtc ctcagaggcg
      601 cggcgtctgc actcaggtca agaccactac cccgaagaag cccaactcgg ccctacgaaa
      661 gatcgcacga gtgcgtctga cgtccggcgt cgaggtaacg gcctacatcc caggcatcgg
      721 gcacaacctt caggagcact cgatcgtgct tgttcgtggg ggccgtgtga aggacctccc
      781 tggcgttcgc tacaagatca tccgaggcac actcgacacc gccggtgtac gcgaccgcaa
      841 gcaggcccgc agccgctacg gcgcaaagag agcctcctga tgccacgcaa aggaccagct
      901 accaagcgcg aggccgtctc agacccggtc tatcagagtg tgctcgtcac tcaattcgtt
      961 aaccgcatca tgctcggcgg caagaagaca ctcgccgaga aggttgtgta cggggccctc
     1021 aagacggttt ccgagaagac cggcggcgac ccaactgctg tgctccgcaa ggccgtcgag
     1081 aacgtcaggc cacttgtcga ggttcgctcc cgtcgtgtgg gtggttcgac ctatcaggtg
     1141 cctgtcgagg ttcccagtcg ccgttcgctg acgcttgcga tccgctggat ggtcaacttc
     1201 tcccgtgccc gcaaggagcg gacgatggca gatcgtctag cagccgagct gatggatgca
     1261 tccaatggtg caggggcctc ggtaaagcgt cgtgaggacc ttcacaagat ggcggaggcg
     1321 aacaaggcgt tcgcacacta cagatggtga tcgacacccg ttgaccctgc tggagcgctg
     1381 atgcgctgct gaggggtcga caggccgggg ggaaccccgg tcgtttcatg tgtgggctga
     1441 aaactggata gaggaatcga tggtaacgac cgtggacgac gaagagaggc agcaagcgat
     1501 cgaggccaag cgccgcgctt ctgttctgcg cgagttcccc ttggcccgta cccgcaatat
     1561 cggcatcatg gcccacatcg acgcgggtaa gacgacgacg accgagcgca tcctctacta
     1621 caccggccgt acctacaaga tgggcgaggt ccacgagggc ggcgccgtca tggactggat
     1681 gatccaggag caggaacgcg gcatcaccat cacatcggcc gcgaccacga ccaagtggaa
     1741 agaccactgg atcaacatca tcgacactcc tggtcacgtc gacttcaccg ttgaggtcga
     1801 acgttctctg cgggtcctcg acggtgcgat agcggtgttc gatgccgtcg ccggcgttga
     1861 accgcagacc gaaaccgtct ggcggcaggc cgatcgctac agcgttcccc gcatatgttt
     1921 cgtcaacaag atggaccgcc tcggcgcgga cttcttcatg accgtttcta tgatccgaga
     1981 gcgtctcggt gggcaccctc tgcctgttca gatcccgtgg gggagtgagt ccgactttca
     2041 cggcgtcatc gacctcgtcg agatgaaggg ccgctactgg accaccgaga agggcgagga
     2101 gtgggaggac cgcgaggtcc ctgcagagct tctcgatgaa gctaaccatt ggcgtcacga
     2161 gctttatgag gcccttgccg agtacgacga agaggtcatg gacgcatacg tccacggcgg
     2221 cgacccaacc cccgagcatc tgcgtctggc acttcgcaag gcaacgctgt cgggtacagc
     2281 tcaaccggtc ctctgtggat ccgcattcaa gaacaaaggc gtgcagcccc tgctcgacgc
     2341 tgtcgtcgca tatctgcctt caccgctcga cgtcaaaccg gtggtcggcg agaacccgag
     2401 gacgggggcc gaggagatca ggcgtcccga cgacggcgag cccttcgccg ctctcgcgtt
     2461 caagatcatg tccgacccgc atgtgggccg catcacatac atgcgcgtct actcagggag
     2521 cttgaagtcc gggggtcacc tcctgaactc gacccgcgac cgcaaggaac gtgctggtcg
     2581 gcttctgcag atgcacgcca accacagaga agacaaagag tccgttctaa cgggtgacat
     2641 cgtcgcgatc gttggcctca agaacacgat cacgggagat accctctgcg accccgacca
     2701 ccccatcagg ttagaggccc ccatcttcgc ccagccggtc atctcgctcg cgatcgaacc
     2761 gaagaccaag gccgaccaag acaagct
//
LOCUS       DATDOZ010000153         6562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1446913, whole genome shotgun sequence.
ACCESSION   DATDOZ010000153 DATDOZ010000000
VERSION     DATDOZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446913"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VI541_02955"
     CDS             complement(<1..531)
                     /locus_tag="VI541_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69897.1"
                     /translation="MRARSLALSMLVLLLAAGCSKQTSAQTPIYAPSLRVGLLITTGG
                     IGGDLAGPTIGAANVAADHLKQDHNVAVTLEQADYQGDLTAVPRMLADLAKKVDVIIV
                     GTDDASVIPDLAAVSVPVIDAYLSSQQAGSAPNIFSLGPTDEDQARRLVTYLISTRKL
                     GNLAVIFEETPYGLRGA"
     gene            complement(528..1202)
                     /locus_tag="VI541_02960"
     CDS             complement(528..1202)
                     /locus_tag="VI541_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69898.1"
                     /translation="MRTILLRLALAFTLMMSVACQRTVAGSDAKVEQSEAPVLGGGEE
                     AGQQLDTGAALGGGQQAASVSEAAPPGLRIVNMKISPTTLGIIQSTGFEGFRSGDTSS
                     LIQLPLYGLDPRLNIYYLVVAVGNFGSAPVRNLKGRAEFFDAKGVKVWSETQAITHLP
                     TRLGLNPPSLPNVLEPQAELGAELRGFGIYYFPTNVGAFTFSVPDSAVSATIKSWKLV
                     FLVSTT"
     gene            complement(1199..3481)
                     /locus_tag="VI541_02965"
     CDS             complement(1199..3481)
                     /locus_tag="VI541_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69899.1"
                     /translation="MLGVGFPLNGGGASLQLSTRRMAGGGKMKWLRLQRRHRRIAGVL
                     VTAILTTNLLVLSSTAQVPSPTANPASNAPTSTSTTDTGAGSPAAKPSSITDTEYISV
                     RIDANGAPVEVNVKEWYRVRSDKGASVRIVDPGVLAGVRSLGGGKPEVREDAMSMDVG
                     VPANKPGCRGGCGYKDVYFAGEPIAREGGTLRTASGPHGLPIEISIRFYIGDPGSNDA
                     TQQVNARKFTEHRGPFKLVITVRNNTKRTQEVTYNDVQTKKAMTGIAEVYQPFVVRIP
                     EIELPDTNYDSLRSNGIIGRRGKASVVSWNLNLAPPDYPGTQDAILEGRSSAPSIPSI
                     SVIAQPIYPEPVAEALSSSGQQFQKGRRSFNYDVLNLFRENLVALGGLFAVLDDSFSN
                     LALPLIGPEKGNRDSGSFTDANLLWGMWTVAKGTEQLSRAMDDLTYSIQLARDGIKGQ
                     IATFQLLRAFLGKSTDTPVLASQTGGTPCVPFATCTPEETEGQVNTLLSESIWANLKD
                     LEVTLGSPTNPAVPGSAGDPRPYLPEAPASLATNGALAITIIKIKLAILEHNFYCLQY
                     EDHTNDCSTILGITNKPGATGWDKFSFVKFPFGQLEVEKGLRAIETEGTAQIQAALGN
                     KIQPNSFVWGAKTLVEGIEALIDSFHQLGATWRYVSDSITNFGIFGVDTSKSILQLDI
                     NAIDIQTATRAAAVRRALDNDTFFGKPAGSQGQLVLSFSTDPPPPVERTGTQSVAIGF
                     AVLSLLSVGALFARFRLYWL"
     gene            3559..5127
                     /locus_tag="VI541_02970"
     CDS             3559..5127
                     /locus_tag="VI541_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69900.1"
                     /translation="MLSVFDFVDDWMHRGGDFAADDINSSGGINGRPVQLVYANDPAL
                     GDQGVKQLVGQGIDVLIGPEVFSATQQNTALLKSRKIVNILPLSPVGEIRDLGNPYVF
                     RLLPNDRNQAEALVRHLVDEKGMRRIAVLYEDDFLGRPGVGLVRDQLKFRGLSPVKAI
                     SFNRGDTDFTSQVASVAQTHPDALIVWGLAREAAQAAIAVRDLGLKVKLAMPIEAAAG
                     DFVELAGTAADGAVSVLPHKTINNWAPPGSWRANWFARYHRRYVIQAYRGTKIPNLPI
                     AQAVVYDAVMLYAQAARRAGSTDPDAVRKELESGRKFESVTHDFSFTPGDHETYTSDE
                     LWAFRIEKGAATFEEDPRANIEEEHKAWQLFSLGLLFDRKKGVALIPFSIGIFDAPGP
                     VKVGSLRWRVLGVRFVDSIDAGVFGLPPRKPKGRFAIVDLEVTNASRSVRKAPWTFAV
                     DQDGNLSIPDTEAAAGLWIAGGPAADFFMFHDLSPGASVRAPMVFDVARGARNLQIGI
                     PEDFIFSNYAMVKT"
     gene            5124..6476
                     /locus_tag="VI541_02975"
     CDS             5124..6476
                     /locus_tag="VI541_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69901.1"
                     /translation="MIRCRLAPVLLVAAIAVIPGCSGRGGQVTITVIGPSSGPQSRLG
                     IEARRGAQLAAARIDASGGLLGHPVRIIFRDESRRTDAVSLLRDVVARDRPLAVIGPT
                     DTGSLVSGNDPLSAAGIAAITITGSQGSVAANAHLFRLVPSNAVASDAIVGWVVGDRR
                     FSKVAVVSGEDAQSVDGARAIASNMRKRAVSAVVTTQIGSGLTDLTTVAQSLRSAGAD
                     AVILWAPALESARFMSAVSAIGWSPQIVGPQSWIDDSLRSVAGSATDNVAFASESIAP
                     EVWFSDEMRKWFLDYHRRFTVAPIPSQRTLVTDLSVTALKAFDAVGLIADAAKRADSL
                     DAEDISRELARTRSFKGLLRTYSFGRAGEAIDAKHVTIARFYNLAVLYDVVAPFDAAG
                     QVAFYKVQVSAFGVPPEYQDTPKGREVARQLLEDVLTDPEKIDFFRPYVAPKPAPGHI
                     "
BASE COUNT         1167 a         1975 c         2074 g         1346 t
ORIGIN      
        1 ggctccgcgt aagccgtaag gagtctcctc gaagatcacg gcgaggtttc cgagttttcg
       61 tgtcgagatc aggtaggtga caagccgccg cgcctgatcc tcgtctgtcg ggccgaggct
      121 gaagatgttc ggagccgaac cagcctgttg cgatgagagg tatgcgtcga tgacgggcac
      181 cgataccgcc gctagatcag gtatgacgga ggcgtcgtct gttccgacga tgatcacatc
      241 gaccttcttg gcgaggtcgg ccagcattcg cgggacggcg gtgaggtcgc cctggtagtc
      301 agcctgctca agtgtcaccg ctacgttgtg atcctgtttg agatggtctg cggcaacgtt
      361 cgctgcacct atcgtcggac cggcgaggtc gccgccgatg ccgccggttg tgatgagcag
      421 tccgaccctg agcgaaggcg cgtagatggg ggtttgggct gatgtctgct tgctgcaccc
      481 tgccgccagc aacaggacga gcatagacag cgctagtgat cgagccctca tgtcgtgctc
      541 acaaggaaga cgagtttcca tgacttgatc gtggccgata ccgcggagtc gggcacgctg
      601 aaggtgaagg caccgacgtt cgtcgggaag tagtagatgc cgaagccccg cagttcggcc
      661 cccaactcgg cttggggctc aaggacattc ggaagcgagg gtgggtttag tcccaggcgt
      721 gtcggcagat gtgtgatcgc ctgggtctcg ctccaaacct tcacaccctt cgcgtcgaag
      781 aactctgcgc gccccttcag gttacggacg ggtgcactgc cgaagttacc gaccgccacg
      841 acgaggtagt agatgttcag tcggggatcg agcccatata ggggaagctg gatcaaagac
      901 gaggtgtcac cgcttcggaa tccttcgaag cctgtcgatt ggatgatccc gagggtcgtc
      961 ggcgagatct tcatgttgac tatccgaaga ccaggaggtg ctgcctccga gacagaagca
     1021 gcctgctgcc caccgccgag tgctgccccc gtgtctaact gttggcccgc ctcttctccg
     1081 ccgccgagaa cgggggcctc cgactgctcg accttggcgt ccgagcccgc gaccgtgcgt
     1141 tggcatgcga cagacatcat gagcgtgaaa gcgagcgcga gccgcagcag gatcgttctc
     1201 ataaccagta caacctgaat cgcgcgaaca aggcccccac ggacagaagg ctcaacacag
     1261 cgaacccgat cgcaacactc tgtgtgcccg tgcgttcgac cggtggtggg gggtccgtcg
     1321 agaaagagag gacgagttgc ccttgagagc cggctggctt tccgaagaag gtgtcgttgt
     1381 cgagggccct gcgaaccgcg gctgcgcgcg tcgccgtctg gatgtcgatc gcgttgatgt
     1441 cgagctgaag gatcgacttc gacgtgtcca ctccgaatat cccgaagttg gttatcgagt
     1501 ccgaaacgta gcgccaggtc gcgccgagct gatggaacga gtcgatgagc gcctcgatcc
     1561 cttcgacgag cgtcttggct ccccatacga atgagttggg ttggatcttg ttccccagcg
     1621 cggcctggat ctgtgcggtg ccctcggtct cgatcgctcg aagtcccttc tcgacctcca
     1681 actgcccgaa ggggaacttc acgaatgaga acttgtccca gccggtggct ccaggcttgt
     1741 tcgttatccc gaggatcgtg gagcagtcgt tggtgtggtc ctcgtactga aggcagtaga
     1801 agttgtgttc caggatggcc agcttgatct tgatgatcgt gatggcaaga gcgccgttcg
     1861 tcgccagcga agcgggggcc tcggggagat aggggcgcgg atcgcccgca ctgcccggca
     1921 ccgcagggtt ggtcggcgac cccagggtga cctcgaggtc cttgaggttc gcccagatgc
     1981 tctccgagag aagtgtgttg acctgccctt cggtctcttc gggagtacag gtagcgaacg
     2041 gcacgcaggg cgttccgccg gtctgggatg ccaggacggg tgtgtccgtg gactttccca
     2101 ggaaagcgcg cagtagctgg aaggtcgcga tctggccctt gatcccgtcg cgtgcgagtt
     2161 ggatggagta ggtgagatcg tccatcgccc gtgatagctg ctcggtcccc ttcgcaacgg
     2221 tccacatccc ccacagtagg ttcgcgtctg tgaacgatcc tgagtcacgg ttccctttct
     2281 ctggtccgat caacggaagc gctaggttcg agaaggagtc atccagaacg gcgaagagtc
     2341 cgccgagcgc gacgagattc tcgcggaaga gattcagcac gtcgtagttg aaagaacgtc
     2401 ttcccttctg gaactgctga ccgctcgaag agagtgcctc ggcaacgggc tccggatata
     2461 tcggttgggc tatcacgctg atgctcggga tcgagggcgc gctcgaccgg ccttcgagga
     2521 tcgcgtcctg cgtccctggg tagtcgggtg gcgcgaggtt caggttccaa ctgaccacgc
     2581 tcgccttgcc ccgccgtccg atgatcccgt tcgagcgcag gctgtcgtag ttggtgtctg
     2641 gtaactcgat ctcagggatg cgcaccacga acggctgata cacctcggct atccccgtca
     2701 tcgccttctt cgtctgaacg tcgttgtaag tgacctcttg tgtccgcttc gtgttgttgc
     2761 gaactgtgat gacgagtttg aacgggcccc gatgctcggt gaacttgcgc gcgttcacct
     2821 gctgcgtcgc atcgttcgag cccgggtcgc cgatgtagaa gcggatcgag atctcgatgg
     2881 gcagaccgtg aggaccactt gctgtccgca acgttccgcc ctcgcgcgcg atcggctcac
     2941 ctgcgaagta gacatcctta tatccgcacc cgccgcggca tccgggcttg ttggctggga
     3001 ccccaacatc catcgacatg gcatcttcac gcacttctgg cttgcctcca cccagtgaac
     3061 gaacgcctgc cagcacacct gggtcgacta tccgaacgct agcgcccttg tcgcttcgca
     3121 cccggtacca ctctttgacg tttacctcga cgggtgcccc attcgcatcg atcctcactg
     3181 agatgtactc ggtatcggtg atgctgctcg gtttcgctgc aggactgccg gcccctgtgt
     3241 cagtcgttga ggtcgaggtg ggtgcgttgg aagccgggtt cgcagtgggg ctggggactt
     3301 gagcggtcga ggaaaggacc agcagattgg tagtcaagat cgctgtgacg agaacacccg
     3361 cgatccgccg gtgacgacgt tggagcctga gccacttcat ctttcccccg cccgccatcc
     3421 tcctggtgct aagttgaagc gatgcgccgc cgccgttcag cgggaatcct actcctagca
     3481 tttgtctggg caaccctagt cgaaggcgct ccctcgacat cgcgcgccgc cgtctcgggg
     3541 cccccgatcc ggatcgggat gctgtctgtc ttcgacttcg tcgatgactg gatgcacagg
     3601 gggggtgact tcgctgcaga cgacatcaac tcatcggggg gcatcaacgg gcgccccgta
     3661 cagctcgttt acgcgaatga cccggccttg ggtgaccagg gggtcaagca gctcgtcggg
     3721 caggggatag atgttctcat cgggcccgag gtcttcagcg cgacccagca gaacaccgcg
     3781 ttgctcaaga gccgcaagat cgtgaacatc cttccactct ctccggtcgg cgagatccgt
     3841 gatctcggga atccatatgt ctttcgactc ctcccgaacg accgcaatca ggccgaggct
     3901 ctcgtcaggc atctggtcga tgagaagggg atgaggagga tcgcggtcct gtacgaggat
     3961 gattttctcg gcaggccggg ggtgggattg gttcgggatc aactcaagtt ccgcggattg
     4021 tcgccggtca aagcgatctc attcaaccgc ggtgacaccg acttcacttc acaggtggca
     4081 tcagtcgccc agacccatcc cgacgcgttg atcgtgtggg ggctagcccg tgaagctgct
     4141 caagcggcta tcgccgtgcg ggatctgggc ctgaaggtga agcttgcgat gcccatcgag
     4201 gcggcggcag gtgacttcgt cgagcttgca gggacggctg ctgatggcgc ggtctcggtc
     4261 ctgccccaca agaccatcaa caactgggcc ccccctggct catggcgagc gaactggttc
     4321 gcccgctacc accggcgcta tgtgatccag gcatacaggg ggaccaagat cccgaacctc
     4381 cctatcgctc aggccgtcgt ctatgacgcg gtgatgctct acgcccaggc tgcccgaaga
     4441 gcgggcagca ccgaccccga tgcggtgagg aaggagctcg aaagcggccg caagttcgag
     4501 tcggttacac atgacttctc cttcactccc ggggatcacg agacctacac gagcgatgaa
     4561 ctctgggctt ttcgcatcga gaagggcgcc gcgacgttcg aggaagatcc gcgggccaac
     4621 atcgaagagg aacacaaggc ctggcagctc ttctcgctcg gtctcctctt cgatcgcaag
     4681 aagggggtgg cgctcatccc gttctccatc ggcatcttcg acgcgccggg gccggtcaag
     4741 gtcgggtcgc ttcgatggcg agttcttggg gtccgcttcg tcgactcgat cgatgccggg
     4801 gtgttcggcc ttcctccgcg aaaacccaag ggccgcttcg cgatcgttga tctggaggtg
     4861 accaatgctt cgcggagcgt gaggaaagcc ccgtggacat tcgcagtcga tcaggatggc
     4921 aacctctcga tccccgatac agaggctgcc gccggtctat ggatcgctgg gggcccggcg
     4981 gccgacttct tcatgtttca cgatctgtca cctggggcct cggtgcgcgc tccgatggtc
     5041 ttcgatgtgg cgcgaggagc taggaacctt cagatcggga tcccagagga cttcatcttc
     5101 tcgaactacg cgatggtcaa gacatgattc gctgtcggct agctcccgtg ctgctggttg
     5161 ctgccatcgc cgtcataccg ggctgttccg gtcgtggcgg tcaggtgacg ataactgtaa
     5221 tcggcccatc ctcaggcccc cagtcgcgcc tggggatcga agcgcgaagg ggggcccagc
     5281 tcgctgcggc tcgtatcgat gcttcaggcg gattgctcgg gcacccggtc aggatcatct
     5341 tcagagatga atctcgtcgt actgatgccg tgtcgctcct aagagatgta gtcgctcgcg
     5401 atcgacccct agctgtcatc ggcccgacgg ataccggttc tctcgtttcg gggaacgatc
     5461 cgctctcggc cgcaggcatc gcagcgatca ccatcacggg gtcccaaggc tcggttgctg
     5521 caaacgccca cctgttcagg ctggtccctt cgaacgctgt tgcgtctgat gcgatcgtgg
     5581 gttgggtggt cggggacaga cgattctcga aggtggcagt cgtgtcgggt gaggacgcgc
     5641 agagcgtcga tggcgcaagg gccatcgcat cgaacatgcg caagcgcgcg gtatcagccg
     5701 tggtcacgac gcagatcggg tcgggtctca ccgatctcac gacggtggct cagtcgcttc
     5761 ggtcggcggg ggccgacgcg gtcatcctgt gggcccccgc tctcgaatcg gccagattca
     5821 tgagcgcggt gagcgcgatc ggctggtcgc cccagatcgt gggaccgcag tcctggatcg
     5881 acgactcact gcggtcggtt gcaggttcag caaccgacaa cgtcgccttc gcatctgaga
     5941 gtatcgctcc ggaggtctgg ttcagcgatg agatgcggaa gtggttcctc gactatcacc
     6001 gcaggttcac cgtcgcaccc atccccagcc aacgcacgtt ggtcacggac ctttctgtga
     6061 ctgccctcaa agcattcgat gcggtcggcc tgatagccga cgccgcgaag cgcgcagatt
     6121 ctctcgacgc agaggacatc tcgcgagagt tggcgcggac tcgctccttc aagggactgc
     6181 tccgcaccta ctccttcggc cgtgctggcg aagctatcga tgcgaagcat gtgacgatcg
     6241 cgcgcttcta caacctcgcc gttctctacg atgtggtcgc gccattcgat gccgcgggac
     6301 aggtcgcctt ctacaaggtg caggtctcgg ccttcggagt cccgcctgaa tatcaagaca
     6361 cgcccaaggg cagggaagtg gcgcgccaac ttctcgaaga cgttctgacc gacccagaga
     6421 agatcgactt cttcaggccc tacgtcgccc cgaaacccgc tccgggacac atctagatgg
     6481 ctctctttga gcaggctctg gtcaccggtg ttgggacggg agcgatctat gcgttgatcg
     6541 cctacgggat ctcgctggtt tg
//
LOCUS       DATDOZ010000154         4525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2748143, whole genome shotgun sequence.
ACCESSION   DATDOZ010000154 DATDOZ010000000
VERSION     DATDOZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748143"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VI541_02980"
     CDS             <1..1736
                     /locus_tag="VI541_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF69902.1"
                     /translation="GDSALKARVIARLAEATRFSDLSRERELSREAVETARRSGDNTA
                     LAQALYSRSISLGSPWTVQERLEAASELIAAAEQANNRNLQMLGYRIRTSVFGELGRM
                     AELADDAVAYAKIAAESRQPLYIWWTSMYAGMRALMDADLEKAETIMREAWNLGTAAH
                     DPNAEIVLIPPRFLLARESGNFDGIRDHFDVLVRKGVSDFIIWRGSVFYFQLLTGDFS
                     AVERSLPTIVAHIETEERDWGWCWWAYEVAEILTHLGESEQARQLYEKVLPFADQVAT
                     PGYSTACYGSNHHTLGLLSRTMGDLDVSRDHFARAVKVNAAMSAPGLVARSKYELART
                     LVASKTDGARHEALSLLGESLDICRERKLGGLVDKVLDLKLQVQGVDRSDVQTSIHAV
                     ADEVAKEQPDLGGAASPDGTITIVFSDIEGSTAMNERLGDQRWVELLRGHDAIVRNET
                     AAAGGVIVKSRGDGFMLAFSSARQALRCAIGIQHGFAKHNQNAETPIRVRIGVHTGEV
                     VKEAGDFYGRHVNLAARIADQADGDEILVSELTSQLLAGSQEFRFADSRESELKGFPG
                     RHRMIRVDWRD"
     gene            complement(1733..2782)
                     /locus_tag="VI541_02985"
     CDS             complement(1733..2782)
                     /locus_tag="VI541_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLF69903.1"
                     /translation="MTQPQPRTHIYRVILTTIWLTIASGLVLLLVVALRKVIFLVVVA
                     TFVALALNPAVRAITRWKIKRGVAVLIVVLAGLISAGAIGGLLASSLAGQGVQFAQQA
                     PKYLQESVNGKGPVFALARRLNLEGELRKAGPAMSDAVSKLPVELVRLLSSIASATAS
                     AVVIAVLSVFLLIEGPNIVSGVLRLLSREQRAPARRFGRSTSSIVSGYMTGVFGLAAI
                     NGLITAATLALLGVPFVVPLAIWAGLADVLPIVGGLLGIVPAALFAFAHSTSAGIIAV
                     LVMLAFQQIKNHFLYPVIIGRAAKMNPLVVFLAVLVGAELGGIGGAILAIPIGGAIQS
                     FAIEFVAPLRDGPDN"
     gene            2863..3633
                     /locus_tag="VI541_02990"
     CDS             2863..3633
                     /locus_tag="VI541_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF69904.1"
                     /translation="MSKVRVSSSGPVLVLTITRPQVRNCVDGETATEIGRAIEEFAHD
                     PDHHVMVITGEGDKAFCAGADLRNADTLMRHEFTDRAGPMGFAALDPGKPTIAAINGV
                     CVAGGLELAAWCDFRIADEKATFGALNRRWGVPFLDGGTQRFPRIVGLGNALYLMETG
                     ARFDAAAALRMGFVQEVVSAGRSLDRALELAQHIASYPQSSIRSDRHATLGGLGAPLA
                     EGLRIEAVLGREVLDDPQMREGLAAFDEGRRPEPPLAP"
     gene            3630..>4525
                     /locus_tag="VI541_02995"
     CDS             3630..>4525
                     /locus_tag="VI541_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017177113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLF69905.1"
                     /translation="MSNVADEDLALFARGKHPDLWNLLGAHPIHRDGSDGVRFALWAP
                     FARHVAIAGDFNDWEPKSAPMSLVDATGVWSAFVEGAKAGHLYKFEVTGPDGDAVLKA
                     DPFARASERPPRTASVITQSSYAWGDADWMERRATTDTLTSPMSIYECHLGSWRHNGS
                     GEPLSYREVAPLLSTYLLDLGFTHIEFMPLAEHPFRGSWGYQSSSYFAPTARFGNPDD
                     LRYLIDHLHRCGIGVIFDWVPGHFPRDDFALARFDGTALYEYDDPRRGESPDWGTYLF
                     DLTKPQVRNFLIANALYWLEEF"
BASE COUNT          886 a         1354 c         1446 g          839 t
ORIGIN      
        1 aaggtgatag tgcgctcaag gcccgagtta tcgcccgcct cgccgaggca acccgattca
       61 gcgacctgtc gagggagcgg gaactgagtc gcgaggcagt cgagacggca cgccgatcgg
      121 gcgataacac ggctctggct caggctctct acagcaggtc gatctcgttg gggagcccgt
      181 ggacggtaca ggaacgactg gaagccgcca gcgagttgat agcggctgcc gagcaggcga
      241 acaaccgcaa tcttcagatg ctcggctatc ggatacgaac ctccgtcttt ggggagttgg
      301 ggcgcatggc cgaactcgct gatgacgctg tggcctacgc aaagatagcc gcagagagcc
      361 gccagcctct ctatatctgg tggacatcga tgtatgccgg gatgcgcgca ttgatggacg
      421 ccgatctcga gaaggctgag acgatcatgc gagaggcatg gaacctcggc accgcggccc
      481 acgaccccaa cgccgaaatc gtactcatcc caccacgatt cctccttgcg cgcgagagcg
      541 gcaacttcga tgggatccgg gaccacttcg atgttttggt cagaaaggga gtcagtgact
      601 tcatcatctg gcggggttct gttttctact tccagctgct cacgggcgac ttttccgcag
      661 tagagcggtc gctgcccacc atcgtcgcgc acatcgagac agaagagcgt gactggggat
      721 ggtgctggtg ggcatacgag gtcgccgaga tcctgactca cctgggcgaa tccgaacagg
      781 cacgccaact ctatgagaag gtgttgccct tcgccgatca agtggcgaca ccgggctact
      841 ccacagcctg ttatggaagc aaccatcaca ccctcggtct tctgtccagg acaatgggtg
      901 atctggatgt ctcgcgagat cacttcgcgc gggccgtgaa ggtcaacgcg gccatgagtg
      961 cgcccggatt ggtggcacgc tccaagtacg agctggcgcg gacgctggtg gcatcgaaga
     1021 ccgatggtgc tcggcatgag gccctgtctc tgcttggcga gagcctcgac atctgccgcg
     1081 aacgcaagct cggggggctt gtcgacaagg tcttggatct gaagctgcag gttcaagggg
     1141 tggaccgcag tgatgtccag acctcgatcc acgctgtggc cgatgaggtc gccaaggagc
     1201 agcccgacct agggggtgcg gcttcgcccg atgggacgat caccatcgtc ttcagcgaca
     1261 tcgagggctc aaccgcgatg aacgagaggc tcggcgatca acgttgggtc gagttgctgc
     1321 gtggccacga tgcgatcgtg cggaacgaga ccgcggccgc aggcggcgtg atagtgaagt
     1381 ctcgtggtga cggtttcatg ctcgcattct cgagtgcgcg ccaggctctt cggtgcgcga
     1441 tagggatcca gcacgggttc gcgaagcaca atcagaacgc cgagactccg atccgcgtga
     1501 ggataggcgt tcataccggc gaggtggtca aggaggctgg cgacttctac ggcagacacg
     1561 tgaacctggc cgctcgtatc gccgatcaag ccgatggcga tgagatcctc gtatcggagc
     1621 ttacgagtca gctactggcg ggctcacagg agttccgctt cgcagactcg cgcgagtcag
     1681 aactcaaggg gttcccaggc cgacaccgga tgataagggt cgactggcgg gactagttgt
     1741 ccggtccgtc gcggagaggg gccacgaact cgatggcgaa cgactggatg gctccgccga
     1801 tgggtatggc aaggatggct ccaccgatcc caccaagctc cgcgccaacg agcacggcca
     1861 ggaagaccac gagcggattc atctttgcgg ctctgccgat gatcaccggg tagaggaagt
     1921 ggttcttgat ctgctggaac gcgagcatca ccaagacggc gatgatgcct gcggatgtcg
     1981 agtgtgcgaa cgcgaatagg gctgctggca cgatcccaag gagtccgccg actatcggaa
     2041 gaacatcggc gaggccggcc cagatcgcga gaggaaccac gaagggcacc ccgagcagag
     2101 cgagtgtggc cgctgttatc aggccgttga tcgcggccaa tccgaagacg ccggtcatgt
     2161 agccactcac gatcgatgag gtcgatcgcc caaagcgacg ggctggtgcg cgttgttcac
     2221 gggaaagcag gcgcaacacc ccagagacga tgttaggccc ctctatgagc aggaagaccg
     2281 agagcaccgc tatcaccact gcactggcag ttgcgcttgc gatcgacgag agcagcctga
     2341 ccaactcgac aggcagtttc gagactgcat ccgacatggc tggtcctgcc ttccgtagct
     2401 caccttcgag gttcaacctt ctcgccaagg cgaagacggg gcccttcccg ttcacggact
     2461 cctgcaagta tttcggcgcc tgctgggcga actgcacacc ctgacccgca agagaactgg
     2521 ccagcagacc tccgatggcg cccgcactga tcagtccggc taggacgacg ataagcaccg
     2581 cgactccgcg cttgatcttc catcgtgtta tcgctcgaac ggcggggttc agggccagcg
     2641 cgacaaatgt tgcaaccaca acgaggaaga tcaccttcct aagtgcaaca acaagaagca
     2701 ggaccaggcc gctcgcgatg gtcagccata tcgtggtgag gatcacccgg tagatgtgcg
     2761 tccgtggttg aggctgggtc aactgggcgt ctcctttcgg cgagcgcgat tctagtagga
     2821 cgagagccat gcggggggcg tttgtcgggg tacagtcttg agatgtccaa ggttcgggtt
     2881 tcttcgagcg gtccggtctt agtgctcacc attacgcgtc cccaggtgcg caactgcgtc
     2941 gatggcgaga cggccacaga gattggcagg gcgatcgaag agttcgctca cgaccccgat
     3001 caccacgtga tggtcatcac gggcgagggc gacaaagcct tctgcgcggg tgctgacctg
     3061 cgaaatgccg acacgctcat gcgtcacgag ttcaccgata gggccggtcc gatgggtttc
     3121 gcggcactcg accccggcaa gccgactatc gcagccatca atggcgtctg cgtcgccggg
     3181 ggcctcgagc tcgcggcctg gtgcgacttc aggatcgccg atgagaaggc caccttcggg
     3241 gccctcaatc gtcggtgggg cgttccattc ctcgacggag gcactcaacg tttcccccgg
     3301 atcgtgggtt tgggcaacgc cttgtatctg atggagacag gagcgagatt cgacgcggcc
     3361 gcggcgttgc ggatgggttt cgtgcaagag gttgtatcgg caggtcgatc gcttgatcgg
     3421 gcgttggagt tggctcagca catcgccagc tatccccaga gcagcatcag gtcggaccgg
     3481 cacgcgaccc tcggcggact gggggccccg ctggcagagg ggctgcgcat cgaggcagtc
     3541 ttgggccgag aggtcctcga tgatccacag atgcgtgagg gcctagcggc attcgatgaa
     3601 ggtcgtcgtc cggagccccc gctcgcgcca tgagtaacgt cgccgatgag gacctcgcac
     3661 tgttcgcgag ggggaagcac cccgaccttt ggaatcttct tggcgcacat cccatccatc
     3721 gggacggatc tgacggggtt cggttcgcgc tgtgggctcc tttcgctcgc cacgttgcga
     3781 tagcggggga cttcaacgat tgggaaccta agtcggcccc catgtcgctc gtcgatgcga
     3841 ccggggtctg gtccgctttc gtcgaagggg ccaaggctgg ccacctgtac aagttcgaag
     3901 tgacgggccc cgacggcgac gctgtgctca aggccgatcc attcgcgcgt gcatccgaac
     3961 gtccgccgcg gacggcgagc gtcatcacgc agagcagtta tgcgtggggt gatgccgatt
     4021 ggatggaaag gcgcgcgacg actgacaccc tgaccagtcc gatgagcatc tacgagtgcc
     4081 acctcggctc gtggcgacac aacggatctg gtgagcccct ctcttaccgc gaagtcgccc
     4141 cgctgctctc gacctatctt cttgatctcg ggtttaccca catcgagttc atgccgttgg
     4201 cagagcatcc tttccgaggt tcatggggat atcagagcag ttcttacttc gcccccaccg
     4261 cgcgtttcgg gaaccccgac gacctccgat acctgatcga ccaccttcac cgctgcggga
     4321 tcggggtgat cttcgactgg gtccccggcc acttccctcg agacgacttc gcactcgcac
     4381 gcttcgatgg caccgctctc tacgagtacg acgaccctcg caggggcgag agccccgact
     4441 gggggacata tctcttcgac ctgacgaaac cacaggttcg caacttcctc atcgcaaacg
     4501 ccctctactg gctcgaggag tttca
//
LOCUS       DATDOZ010000155         4120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2230706, whole genome shotgun sequence.
ACCESSION   DATDOZ010000155 DATDOZ010000000
VERSION     DATDOZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2230706"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VI541_03000"
     CDS             <1..1016
                     /locus_tag="VI541_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69906.1"
                     /translation="PPALRSCAGPYSISKGASEGSYTLTRTTRSRSENEAFSSRRGRP
                     KTIRFRAAATPDDAFGLFTKGEVDIAPVPISAFPEVTSRPGFHRAATSEVTYLAFGAN
                     APPTNDRFLRRAVSLAIDRLVVIDAAFGDGREPALRWLKTNTGGEIPPACVSFIRRIS
                     DPARAKSTLSQSVADPSQLKLTLHFDKTDVQRLVVEALQVEMKDILGIQVEVDGQETP
                     GFQQTLQPGAEGVWLLRSDLGISSGHGALGSLLSAGSPLQRLGYADPALDASLLAADS
                     ATGPEQRKAWGTVEEGVCDEMQVTPLWFGVRNWMFAPGIFRKSDGSELGSMGEPRLRD
                     LPV"
     gene            1094..2713
                     /locus_tag="VI541_03005"
     CDS             1094..2713
                     /locus_tag="VI541_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69907.1"
                     /translation="MRSLAHSLAALLMLTSCKIASKPVENSPAIRATHIRVAILDPGK
                     IDPSSASSSSAMLILKQICDTLTIVDPVTGAVKPGIAASWAMAPDAKKVTFKIRPGLR
                     FHNGREVVAADFVTSFSRFASPTSGSSQHFFLEKVVGYQDLRSGRSTNLAGVKAVDAT
                     TLEVGLSEPFAEFPTILSHPAAGAPIPAESLADGGAFAALPDCAGPYVLSSPRTTGDI
                     HLVRAPGYEPSQGATHPDALKGIPEEMTFKVVADVQTGYKELIAKKVDVAEVPTTELL
                     SAQRARGRVESGQNGLMTYIGFPVTRAPYDQLEFRRALTLATNQGAIVEELLAGSRVL
                     PRSFLPLSTGPDSRGLSCASLLDRRSDLVEARKSLKDSGVDVASVTPTVHLNSGAGHE
                     RWVELLTTRWKEQLGVTTTLKAAEWKTYLDGLVDPGPDGPFRLGWASRFPSPESILDP
                     LFRSGSLDNFTRYSNPAFDQALATARSTIDDIKRKKAYRDAADILCKDLPIIPMWEGR
                     NNIAFGPGIASGTGRRLDFDGDPMLRELGRR"
     gene            2737..>4120
                     /locus_tag="VI541_03010"
     CDS             2737..>4120
                     /locus_tag="VI541_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF69908.1"
                     /translation="MLGLLLVGALTSCINGRSVAKPELTIAVERITSLDPLALREPGA
                     MLIDRALFDTIVRFDEDAQLLKPSLAESWETLDSGSRFIFHIDPRAKFHDGAKVRSED
                     VKFSLDRLASASTHSDSAPLLADILGFDDVSVRGVQGLSGVSVIDESSFEIRLASPWR
                     EFPLVLTNLATAPVAKTQVERNPDGLRTSPIGTGPYRLIRSAQGRIDLRRNVHYFGTA
                     PLTDRVSFVSYPNDRAALADLEDGKVDLARAPARSAANAIARFGSAGVVPLSAGIYIG
                     VDVQKVALDTRRRIARSIDRETLSKQALGYAAQPAVSLTLPPGTAKCEECRFDPLAQT
                     NQLSIKIGYPASGSLSEIGDAVADALDAAGIHTERVKSDVATVLAGVDAGDIDAYVFG
                     WSGEYPGPHAFLYPLVRTGSSNNHTHYSVTAVDALIEQFRASPDRVGATEAITAAESR
                     VLADLAVIPLI"
BASE COUNT          800 a         1280 c         1240 g          800 t
ORIGIN      
        1 cacctccagc gttgcgatca tgcgctggcc cttattcgat atccaagggt gcatcagaag
       61 ggagctacac ccttactcgc accacgcgtt cccgctcgga aaatgaagcg ttcagtagcc
      121 gaagaggacg acccaagacc atccgctttc gggctgccgc gacacctgat gatgctttcg
      181 gactgttcac gaagggcgag gttgacatcg ctcccgtacc catctcggcc ttccctgaag
      241 tcacctcacg accaggcttc caccgggccg caacgtcgga ggtcacctat ctggcctttg
      301 gcgccaacgc acccccgacc aacgatcgct tcctgcgaag agcagtatcg ctggccatcg
      361 accgcctcgt agtgatagac gctgcgtttg gtgatgggcg agaaccggct ctgcgttggt
      421 taaaaaccaa cactggcggg gagatacctc cggcctgcgt ttcgttcatc cgtaggatct
      481 cggaccccgc cagggcgaag agcacgctgt cgcagagtgt tgctgatcct tcacagctca
      541 aactcacact gcacttcgat aagacagacg tacaacgcct cgtggtcgag gccctgcagg
      601 tcgagatgaa ggacatcctt ggcattcagg tggaggttga cggtcaggaa actcctggct
      661 tccagcagac cctgcagccg ggcgcggagg gtgtctggct tctacgcagt gaccttggca
      721 tatcgtccgg tcacggcgcg ctcggatctc tgctgtcagc gggctctccg ctgcagaggc
      781 tcgggtacgc agatccggct ctggatgcct cccttctcgc agcggattcg gcaacggggc
      841 ccgagcagcg gaaggcgtgg gggaccgtcg aagagggtgt ctgtgacgag atgcaggtga
      901 ctcctctctg gttcggcgtg aggaattgga tgttcgcgcc gggcatcttc cgaaagtcgg
      961 atggttcgga gctcggttcg atgggggagc cccgcctgag agatctgcca gtctagctag
     1021 gaaccacccc acaggtggtt ggttacagta agaccgacgg gatatctggt tgggcgatgg
     1081 tagattgccc gggatgcgtt cactagccca ctcgctcgct gcgctgctca tgctgacttc
     1141 gtgcaagatc gcgtcgaagc ccgtcgagaa ctccccggcc atacgcgcga cgcacatccg
     1201 cgtagcgatc ctcgatccag gcaagataga cccatcgagt gcgtcctcgt catcggccat
     1261 gctcatcctc aagcagatct gcgacacgct cacgatcgtc gatcccgtca cgggggccgt
     1321 aaagccaggc atcgcagcct cgtgggccat ggcgcctgac gcaaagaagg tcacgttcaa
     1381 gatcagaccg ggcctgcggt tccacaacgg aagagaagtt gtggccgctg acttcgtcac
     1441 ctccttctct aggttcgcct cacccacatc ggggtcatcc caacacttct tcctcgaaaa
     1501 ggtcgtcggc tatcaagacc tgcggtcagg gcgttccacg aaccttgcag gagtgaaggc
     1561 ggtggacgcg acgacgctgg aagtcggcct ttcggagccg ttcgccgagt tcccaaccat
     1621 cttgagccac ccagcagcgg gggcaccgat ccccgctgaa tcgctcgcag atggcggagc
     1681 attcgcggca ctgcccgact gcgcagggcc ctatgtgctg agttcacctc gcaccacggg
     1741 cgacatccat ctggtcaggg cccccggtta cgaaccgagt cagggagcaa ctcatcccga
     1801 tgccctgaag gggatcccag aggagatgac cttcaaggtc gtggccgatg tgcagaccgg
     1861 atacaaggag ctcatcgcca agaaggtcga tgtcgccgag gttccgacca ctgagctgct
     1921 gtccgcgcag agggcaaggg ggcgtgtcga gtctggtcag aacggcttga tgacatacat
     1981 cggatttccg gtgacgaggg ctccatatga ccaactggag tttcgcaggg cccttactct
     2041 tgcgaccaac cagggagcta tcgtcgagga gttgctcgca ggctcgcgag tgctcccaag
     2101 gtccttcctg ccgctttcga ccgggcccga ctcgcgtggg ctctcctgcg catcacttct
     2161 ggatagacgc agcgatctcg tcgaggcaag gaagtcgctg aaggattccg gggtcgatgt
     2221 cgcctcggta accccgacgg tgcatctgaa ctcgggggca ggtcacgaac gatgggtcga
     2281 actcctgaca acgagatgga aggaacagct cggcgtaaca accactctca aggcagccga
     2341 gtggaagacc tacctcgatg ggctcgtcga tccgggtccc gacggaccat tccgactcgg
     2401 ctgggcatcg agattcccct cgcccgagtc gatccttgac ccactcttcc gctccggaag
     2461 cctcgacaac ttcacgcgct actcgaaccc tgccttcgat caggctctgg caacagcgcg
     2521 atctacgatc gatgacatca agaggaagaa ggcctacagg gatgccgccg acatcctctg
     2581 taaggacctc cccatcatcc ctatgtggga ggggcgcaac aacatcgcct ttgggccggg
     2641 gatcgcctcg ggaacaggac ggcggctcga cttcgacggc gacccgatgc tgagggagtt
     2701 ggggcgaagg taggggtggg ccggcctcgg tatcgagtgt tgggcctgct ccttgttggg
     2761 gccctgacct cgtgcatcaa cgggagatct gtagcgaaac ccgaactgac catagcggtc
     2821 gagaggatca caagtctcga cccgttggcc ctgcgcgagc cgggagcgat gctcatcgac
     2881 agggccctgt tcgacacgat cgttcggttc gatgaggacg ctcaactact caagccatct
     2941 cttgcggagt catgggagac gctcgactca ggttcgcgct tcatcttcca catcgatccg
     3001 cgagccaagt ttcatgacgg agcgaaggtt cggtccgaag atgtcaagtt ctcgctcgac
     3061 agattggcca gcgcttcgac acactccgac tcggcccccc tactcgcaga tatcctcggc
     3121 ttcgatgatg tgagcgtgcg cggcgtccaa gggctatccg gtgtctcggt gatcgatgag
     3181 tcgagtttcg agatccgcct agcatctccg tggagagagt ttcctttggt tctcaccaac
     3241 cttgcgacgg cgccagtagc caagacgcag gtcgagcgaa acccggatgg tcttcggacc
     3301 tcaccgatcg gcacgggtcc gtaccgtctc attcgctcag cgcagggccg tatcgatctg
     3361 cgcaggaacg ttcactactt cggtactgcg ccactcaccg accgggtctc attcgtctct
     3421 tacccgaatg atcgtgccgc gctcgccgac ctagaggacg ggaaggtcga cctcgcgagg
     3481 gcgcctgcga ggtcagcggc aaacgccatc gccaggttcg gatcagcggg tgtcgttcct
     3541 ctgagcgctg gtatctacat cggagtcgat gtgcagaagg tggctctcga tacgcgtcgg
     3601 aggatcgccc gaagcatcga cagggaaacc ctctcgaagc aggccctcgg gtacgctgct
     3661 caacccgctg tgtcgcttac cctgccaccg ggtactgcca agtgcgagga gtgccgattc
     3721 gatcccctgg ctcagacgaa ccaactctcg atcaagatcg gatatccggc ttcgggctct
     3781 ctgtcagaga taggtgacgc tgtcgcagat gctctggatg ccgctggcat ccataccgag
     3841 cgcgtgaagt ctgatgtcgc aaccgtcctg gcgggtgtcg atgctggcga catcgatgca
     3901 tatgtcttcg gatggtcagg ggagtatccc ggcccccacg ccttcctcta tccgctggtc
     3961 cgaactggct catccaataa ccacacgcac tactcggtca ccgctgtcga cgcgctcata
     4021 gaacaattcc gagcgtcacc cgatcgagtg ggggcgaccg aagcgatcac ggcagccgag
     4081 agcagggtcc tcgcggacct ggcggtgatc cccctcattg
//
LOCUS       DATDOZ010000156         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3521842, whole genome shotgun sequence.
ACCESSION   DATDOZ010000156 DATDOZ010000000
VERSION     DATDOZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521842"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..982)
                     /locus_tag="VI541_03015"
     CDS             complement(176..982)
                     /locus_tag="VI541_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF69909.1"
                     /translation="MSYLEIAGKRFHYAEAGSGVPVLYLHGFPTSGHLWRNVTPVVAG
                     EYRTICPDLPGFGDSDLLDGPHGWRELTSWIDDFVTALDIAPLHLYVHDWGGLVGLPW
                     LCDHPDKARSVTISDTSFNARDRWHAMATAWRTPEVGEQMLGEAQFEPFIGMLSTAVA
                     QPLDDEAARHYWKGMATPERRAAKLEMYRSLDFEMFEPYMQPFLAAAAGRARVIWGEK
                     DPFLPAKTATELAERLGAELTIVEGAGHFIQEDAGEIVGKLHLEFLSSQG"
     gene            complement(979..2121)
                     /locus_tag="VI541_03020"
     CDS             complement(979..2121)
                     /locus_tag="VI541_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69910.1"
                     /translation="MDFNLPDEVVAFADSVRSFADREISPLVDDAEAKQRTPIELFPK
                     AGKNGFLGLRYPVDVGGAGAGMLAEMTLRMELSKVCAGIASALSVSSHLGSYPIFALG
                     NPDQVDRWLRPILAGEKITSFGLTEPAAGSDVRGIQATAVRSDAGWVLNGRKMFITNG
                     PICDLSIIVAYVDKSAGIDGMALFVVEQGTEGFDSTRRLEKMGHYSSEIGEIVLENVE
                     VPAQNLLGPERGGFHNVMKTLNGGRIVVAGGAIGVAEAAFEVARRYVTEREAFGRKIA
                     GFQAVQHKLADMALDIQAAKQLTQWAAWLYDQDRNPTWEASMAKLYATEMVLRVTDLS
                     LRLFGGYGYIKEFPIERYYRDARMFNIVEGVSDIHRNVIARSLGVV"
     gene            complement(2142..>3007)
                     /locus_tag="VI541_03025"
     CDS             complement(2142..>3007)
                     /locus_tag="VI541_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HLF69911.1"
                     /translation="IEEGTGKMGLNTQLAGKKYEASVVTVTDEAIRNYAAATNEDNPA
                     FVGSDPFAPPAFPIVPAAAAIASVMFDQELGVNLPLLVHGEEDHLFHAPIRAGDQLTV
                     QSTLESVELKETGETFTILTTLTRPDNELVAEVRSLMFIRGSGSGSGSKATSAPAPER
                     AEPLFQTVQKIDDDQTYRYAEASGDHNLIHVDPDFAVNMAGLPGIIVHGMCTMAFAGK
                     AVLDALAGGDPKRLRRIKVRFSKPVFPGQTITTQGWLSSKADGVSTFDFETINPDGVP
                     VIRNGVAEVVD"
BASE COUNT          579 a          942 c          907 g          579 t
ORIGIN      
        1 gagggggccc ccgactcgct cgcgacaact cgtttcacca gcgcgtcgat gctgcttatc
       61 ttcgagctca acccatcgag cttgtgacct gcgctctggc ctcgcgtgta gccgatggca
      121 gccagtgtca atgctcctgc cgacaacgag agcaccagca gaagtgccag gcgtttcatc
      181 cctgtgagct caagaactcg aggtggagct tcccgacgat ctcgccagca tcctcttgta
      241 tgaagtgccc ggccccctcg acgatcgtga gttcggcacc gagtcgctcg gccaactccg
      301 ttgcggtctt agccgggagg aagggatcct tctcgcccca gatgacgcgc gcacgtcctg
      361 cggcggccgc gaggaatggc tgcatgtacg gctcgaacat ctcgaagtcc aatgagcggt
      421 acatctctag tttcgccgca cgacgctcgg gcgtcgccat gcccttccaa tagtgacgcg
      481 cggcctcatc atccagaggc tgcgccacgg ctgtgctgag catgccgatg aacggttcga
      541 actgcgcctc gccgagcatc tgctcgccga cctccggagt cctccacgcg gtagccatcg
      601 cgtgccagcg gtctctcgca ttgaaactcg tatccgagat cgtgacgctg cgagccttgt
      661 ccggatgatc acatagccag ggcaggccta cgagccctcc ccagtcatgg acgtagagat
      721 gcagaggcgc tatgtcgagc gcggtcacga agtcgtcgat ccacgatgtg agctctcgcc
      781 acccgtgagg tccatcgaga aggtctgaat caccgaagcc gggcaggtcg gggcagatgg
      841 ttcggtactc ccctgcgacc acgggtgtca cgttgcgcca gagatgtccg ctcgttggaa
      901 agccatgcag atacaggacc ggcacacccg agcccgcctc ggcatagtgg aaacgcttcc
      961 ccgcgatctc gaggtagctc aaacgacacc aagcgaacgg gcgataacgt ttcgatggat
     1021 atcagagaca ccttcgacga tgttgaacat ccgcgcatcg cggtagtagc gctcgatggg
     1081 aaactccttg atgtaaccgt atccaccgaa gagtcgcaga gacaggtcgg taacgcgcag
     1141 gaccatctcg gtcgcgtaga gctttgccat cgaggcctcc catgtcgggt tgcggtcctg
     1201 gtcgtatagc caagctgccc actgcgtcag ctgtttcgcc gcctggatat cgagagccat
     1261 gtcagcgagt ttgtgctgaa cggcttggaa cccagcgatc ttgcggccga aggcctcgcg
     1321 ctcggtcaca tatctgcggg ccacttcgaa agcagcctca gcgacaccga tcgcaccacc
     1381 ggccactacg atccggcccc cgttcagcgt cttcatcacg ttgtggaagc caccgcgctc
     1441 tggcccaagc aggttctgcg ccggcacctc gacgttctca agcacgatct cgccgatctc
     1501 cgatgagtag tgacccatct tctcgagacg ccgggtggag tcgaaacctt ccgtcccctg
     1561 ctcgaccacg aagagggcca tgccatcgat acccgctgac ttgtcgacat aggccacgat
     1621 gatcgacaga tcgcaaatcg gcccgttggt tatgaacatc tttcgaccgt tcagtaccca
     1681 acccgcgtct gaccgtaccg ccgtcgcttg gataccccga acatccgagc cagcggcagg
     1741 ttcggtgaga ccgaaggatg tgatcttctc gccagcaagg atggggcgca gccatcggtc
     1801 aacctggtcg gggttaccta aggcgaagat cgggtacgaa ccgagatgag aagagaccga
     1861 gagtgctgac gcgatccccg cgcagacctt ggaaagctcc atgcgaaggg tcatctccgc
     1921 gagcatcccg gcgcccgcac cgccgacatc aaccgggtaa cgcaggccga ggaacccgtt
     1981 cttcccggcc ttagggaaga gttctatggg ggttcgctgt ttggcctccg cgtcatctac
     2041 gagaggtgaa atctcgcggt cggcgaatga acgcacggaa tctgcgaagg cgactacctc
     2101 gtcgggcagg ttgaagtcca tgccctggat actacccgcg cctagtcgac gacttcggcc
     2161 actccattgc ggatcacggg gaccccgtca gggttgatgg tttcgaagtc gaatgtgctg
     2221 acaccatcgg ccttcgacga gagccatccc tgtgtcgtga tggtctgacc agggaatacg
     2281 ggcttgctga agcggacctt gatccggcgc agccgcttgg ggtccccacc cgccaacgca
     2341 tcgagaacag ccttgcccgc gaaggccatg gtgcacatgc cgtgcacgat gatccccgga
     2401 agccctgcca tgttcacggc gaaatccggg tccacatgga tcaggttgtg atcccccgat
     2461 gcttcggcat accgataggt ctgatcgtcg tcgatcttct ggaccgtctg gaagaggggc
     2521 tctgcgcgct cgggcgcggg ggcggatgtc gccttcgaac ccgaccctga acccgagcct
     2581 ctgatgaaca tgagcgagcg gacctcggcc acgagttcgt tatcgggacg cgtgagagtc
     2641 gtgaggatcg tgaatgtctc gccggtctcc ttgagctcga ccgattcgag ggtgctctgc
     2701 acggtcagct ggtctccggc gcggatgggg gcatggaaga ggtggtcctc ttcgccatga
     2761 acgagcaggg gaaggttgac tccgagttct tgatcgaaca tcaccgaggc tatcgctgcg
     2821 gcggccggga cgatcgggaa agcgggtggt gcgaaagggt cggaaccgac gaaggcgggg
     2881 ttgtcctcat tggtcgcggc cgcgtagttg cggatcgcct catcggtaac ggttaccacc
     2941 gacgcctcgt acttcttgcc cgcaagctga gtgttcagtc ccatcttccc agtcccttct
     3001 tcgatcg
//
LOCUS       DATDOZ010000157         2851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1706482, whole genome shotgun sequence.
ACCESSION   DATDOZ010000157 DATDOZ010000000
VERSION     DATDOZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706482"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1110)
                     /locus_tag="VI541_03030"
     CDS             complement(4..1110)
                     /locus_tag="VI541_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLF69912.1"
                     /translation="MAKPGDTSADSSEGLRNAGVIAWSLLGILLLGAALLLFLDYLKP
                     IFPPLVLALIIIFLLNPIVTRLENRGVPRLAATVVLYLVFLGLVILLISAMVPAMGSQ
                     LRELGARLPDLRADAVRAINKVMNATGLSTSNVKVEDVVAQTERHLMSGLGQITRITV
                     GALQVLVVFIVGPVVALYLLVDLPKISTAVVGLLPPRYKSELMGLSQQCGDAIGGFFR
                     GQLLVALIVGVLSSVALAVVGLRFWLPIGLLAGFFNLIPLVGPFVGGGVAVVVGASTS
                     GPKTALLAAIAMVIVQQLDNHLISPKVMGRAVSVHPVVVVVALLAGGTLAGLWGMLLA
                     VPSVAVGKIIGGHYYRTRILERVVPDGAPDGAKV"
     gene            1209..1658
                     /locus_tag="VI541_03035"
     CDS             1209..1658
                     /locus_tag="VI541_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF69913.1"
                     /translation="MTVDEMSTKILVVDDEPDVLLLCKVNLEFEGYEVAVAPDGATAL
                     EMIADDPPDLLLLDVMMPGLDGWQVLQRLRDDVEGPQFPVIMLTAKVQEQDQIKGLSG
                     GAVDYVTKPFNPVGLAKTIQEALEAAASGDLEIRRERMLDKIKLIQG"
     gene            complement(1683..2471)
                     /gene="otsB"
                     /locus_tag="VI541_03040"
     CDS             complement(1683..2471)
                     /gene="otsB"
                     /locus_tag="VI541_03040"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HLF69914.1"
                     /translation="MVRRMLDVPTFAEDIRSSPDTSGFILDFDGTLSAIVDDPDAATV
                     VSGVRPILGALADHYGLVAILSGRRAEDVASRVVVPGVVYVGVYGAEQFIDGEITTSS
                     KAPLWREAVVSLSEDVNRLVESARLEGCLVEPKDVALSIHFRNSPSPEAPAVLMSWAR
                     AAVAVHGFTASFGRMVVELKPTEISKAGAVASLIESRGVEFLVLAGDDSADVEAMIAV
                     RATMGDGVLCVGVASSEQPDGLQENADVMLDNSEEVGDLLRRFL"
     gene            2502..>2851
                     /locus_tag="VI541_03045"
     CDS             2502..>2851
                     /locus_tag="VI541_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69915.1"
                     /translation="MRSRKVFIPLIVGLLGTSCTPGAVRLNNHYAARDVYRWRIVTVS
                     RTTGVDLDRSTTQALTMSVTQTARPVPSGTRLMIQLHPNALSIDGIDVDTPRELRIEA
                     TVDGSGKVDITHAPG"
BASE COUNT          650 a          878 c          829 g          494 t
ORIGIN      
        1 atctcatact ttcgcgccgt cgggggcccc atctggtacc acccgttcaa ggatccgcgt
       61 tcgatagtag tgcccgccga tgatcttgcc caccgcaacc gagggcacgg ccaagagcat
      121 cccccaaaga cctgcgagcg ttccgccggc aagtagggca acgacgacga cgacggggtg
      181 aacactgact gctcggccca tgacttttgg actgatgagg tggttgtcga gctgctgcac
      241 gatgaccatc gcgatcgccg cgagcagtgc ggtcttggga ccagaggtgc tagctccaac
      301 caccacagcc acgccgccgc cgacgaaggg gccgaccaga ggtatcaggt tgaagaatcc
      361 ggcaagcaga ccgatcggca gccagaagcg aagccccacc accgcaagag cgacggaaga
      421 caggacccca acgatgagag ccacgaggag ctggcctctg aagaaaccgc cgatcgcatc
      481 tccgcactgc tgggagagcc ccatcagctc cgacttgtat cgcggcggca acaacccgac
      541 taccgcggta ctgatctttg gaagatcgac cagtaggtag agggcgacca caggtccgac
      601 tatgaaaacc actagaacct ggagtgcacc caccgtgatc ctcgtgatct gaccaagtcc
      661 ggacatcaag tgccgctctg tctgcgctac tacgtcttcg actttgacgt ttgatgtcga
      721 gaggccggtc gcgttcatca ctttgttgat agctctgacg gcatctgccc gtaggtcagg
      781 cagcctcgcg ccaagctccc tcaactgact gcccatggcc gggaccatcg ccgagatgag
      841 aaggatgacc agaccgagga acactaagta caggaccact gtggccgcca ggcgaggcac
      901 ccctcgattc tcaagtctcg tgacgatcgg gttgagcaga aagatgatga tcagcgccag
      961 aaccagagga gggaagatgg gcttgaggta gtcgaggaac agaagaagag cggcccccag
     1021 caacaggatg cccagaagag accatgcgat tacccccgca ttgcgaagac cttcggagga
     1081 atctgcggag gtatcacccg gtttggccat caccgaagta tatggaaggg gtcctaacct
     1141 gcataactag gtaaaactga catcgatttg gagcacacct gggctgtagg accggcggca
     1201 tcctgccgat gactgtcgat gagatgagca caaagatcct ggtggtagac gacgagccgg
     1261 acgttctctt gctctgcaaa gtgaacctgg agttcgaggg ttacgaggta gctgttgccc
     1321 ccgatggggc cactgccctc gagatgatcg ccgatgaccc ccccgacctc ctccttctcg
     1381 acgtgatgat gccgggactc gatggatggc aagtgctgca gcgtctccgt gatgatgtcg
     1441 agggtccgca gttccccgtg ataatgctga cagcgaaggt gcaggagcag gatcagatca
     1501 aaggtctgag cggcggcgcc gtcgactacg tgacgaagcc gttcaatccg gtcgggctcg
     1561 ccaagaccat ccaggaagcg ctcgaagcgg cagccagtgg cgacctcgag atccgtcgtg
     1621 agcgcatgct cgacaagatc aaactgattc agggttgatc agggcttgga tctaacgcgc
     1681 cgctagagaa agcgccgtag caggtcgccc acttcttcgg agttgtccaa catcacatct
     1741 gcgttctctt gcagaccatc cggttgttcg gaagatgcga cccctacaca gagcaccccg
     1801 tcccccatcg tcgctcgcac cgcgatcatc gcctcgacgt ctgccgagtc gtcgcccgcg
     1861 agcaccaaga actccactcc ccgagactcg atcagcgatg ccaccgcccc ggccttcgat
     1921 atctcagtgg gtttcaactc gacgaccatc cttccgaagc tcgcagtgaa accgtgtaca
     1981 gcgacggcgg cccgcgccca gctcatcagc accgcgggag cctcgggcga agggctgttc
     2041 cggaagtgga tcgatagtgc gacatctttg ggttcgacca agcacccttc gagccgtgcg
     2101 gattcgacca gccgattgac atcctccgag agggaaacaa cggcctctcg ccacaggggg
     2161 gccttggaag atgttgtgat ctcaccatcg atgaactgtt cggccccata gacgccgaca
     2221 tagacgacac cggggacgac cacccgagag gcgacatctt cggcgcgacg accactgagg
     2281 atcgccacaa gaccatagtg gtcagcaagc gccccgagga tagggcgcac accggacacg
     2341 acggtcgcgg catccgggtc atcgactatc gccgaaagag tgccatcgaa atcgaggatg
     2401 aaacccgaag tgtcgggcga cgacctaatg tcctcagcga aggtcggaac atccagcatt
     2461 ctgcgaacca tactcgacca ccatccccta cactcgctgg agtgcgaagc cgcaaggtgt
     2521 tcatacccct catagttggt ctactaggca cctcctgcac gccgggggcg gtgagactga
     2581 ataaccacta tgcggcccgc gacgtttacc gctggcggat agtcaccgtt tcacggacta
     2641 ccggtgtcga tctggataga tcgaccacgc aagcgctgac catgtcggta acgcagacag
     2701 cgagaccggt accttccgga actcgtctca tgatccaact tcatccgaac gctctgtcta
     2761 tcgatgggat cgacgtcgat acgccacgcg agcttcgcat cgaagcgacg gtcgatggtt
     2821 cggggaaggt cgacatcacg cacgccccgg g
//
LOCUS       DATDOZ010000158         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2486650, whole genome shotgun sequence.
ACCESSION   DATDOZ010000158 DATDOZ010000000
VERSION     DATDOZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486650"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VI541_03050"
     CDS             <1..759
                     /locus_tag="VI541_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HLF69916.1"
                     /translation="GLELGGHVTAEVLMSSVSESLRFVAIIACFFAFIASTSSVDLLR
                     SLPGYLFQAGLALNLALVFAPSLIKTAMGIRDFQRVRHRRARSPIWIVPLLATALERS
                     IAVAESMDSRGFGRRTEAPPAWARGMAAGGGLLLATGGGAFAAGSRSLMAWGVMVAGA
                     ALLAAGLIGLGRSCRRTRLRSDPLQMHDLAVLFAIGGMTLWAVVVAAGGTSLHFDPYA
                     ATDTLFPSTTQVIQMLAIATPAFVYLRQRDRALR"
     gene            756..2309
                     /locus_tag="VI541_03055"
     CDS             756..2309
                     /locus_tag="VI541_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF69917.1"
                     /translation="MIDVTALTYSYPGATSPALDEVSLSLPQGELHVVSGASGGGKST
                     LLRCLNGLIPHFHGGTYSGMVQVDGRSTIDTKPRDLADVIGFMGQNPEDHAVVDRVED
                     DIAFGLENLGVDRSTMRKRVEEALDALGIASLRDRRIDTLSGGERQRAAIAAVMAMMP
                     RYLVMDEPTSQLDPQSAEEVIGAILRLRDEAGLGVVVAEHRLERFLQYAETLTVVDRG
                     RIATGSPGTVLEKSSGGPPIVVLGRAMGWRPLPLGLRDARRLVNSRQRPTRPLPSVAP
                     APPPGGPMISLDSLTVTVAGRPVVRDVDLAVAEGETVALMGRNGSGKSTLLRALTGAY
                     KHHRGRIVGTRSIGYLPQDPASVLFRRSVAEELGSALSTRDPRTIEEAARLFGIEEHL
                     ASYPRDLSGGQRTRVAIASTACMWPRLLLLDEPTRGLDDEGKRYLQWLTETWRDRGGA
                     VVLATHDVELAALVATRVILLSEGQVIVDGTPTQVLADSLTFSTQMNKIFEDPNVLTI
                     TDALAAMRQ"
BASE COUNT          430 a          778 c          751 g          493 t
ORIGIN      
        1 gggctcgaac tcggaggcca tgtcacagcc gaagtcctga tgtcctcggt ctcggagagc
       61 ctgagattcg tcgccatcat cgcctgcttc ttcgccttca tcgcgtcgac atcgagtgtc
      121 gatctgctcc gatcacttcc gggctacctg tttcaggcag ggcttgcgct gaacctcgct
      181 ctcgtattcg ccccatcact catcaagacg gcgatgggca tccgagactt ccagagggtt
      241 cggcatcgca gggcgagatc accgatctgg atcgtccctc ttctggccac cgccctcgaa
      301 cgttccatcg cggtcgcgga atcgatggac tcacgcggct tcggtcggcg aaccgaggca
      361 cccccggcgt gggcacgtgg catggcggcc ggtggggggc tactcctcgc caccgggggc
      421 ggggcattcg cggctggctc gcgcagcctg atggcgtggg gagtgatggt cgctggggcc
      481 gcgctcctcg cggcgggcct tatcggactc ggtcgaagtt gccgacgaac gcgccttcgg
      541 tcagatcctc tgcagatgca cgatctggcg gttctcttcg ccatcggggg catgacgttg
      601 tgggcggtcg tcgttgccgc tggaggaacg tcacttcact tcgaccccta cgcagcgacc
      661 gatactttgt tcccatcaac cacccaagtg attcagatgc tggcgatcgc gactccagcc
      721 ttcgtatatc tccgacagag agacagagca ctccgatgat cgacgtcacc gcactgacct
      781 actcatatcc cggtgccacc tcacccgcgc tcgatgaggt cagcctctcc cttccacagg
      841 gggaactgca tgttgtgtcc ggagcctcgg gcggtgggaa gtcaacactt ctaaggtgtc
      901 tcaacggtct catcccccac ttccacggtg ggacctactc gggcatggtt caggtcgatg
      961 gtcgatcgac gatcgataca aagccgcgtg accttgcgga tgtcatcggc ttcatgggac
     1021 agaacccaga ggatcacgcg gtggttgacc gcgtcgagga cgacatcgcc tttggtctcg
     1081 aaaaccttgg ggtcgatagg tcgacgatgc gtaagcgcgt cgaggaagct ctcgacgcgc
     1141 tcgggatcgc ctcactgcgt gaccgtagga tcgacactct ctcgggagga gagcgccaac
     1201 gcgccgcgat cgcagcagtc atggcgatga tgccgcgcta cctcgtgatg gacgaaccca
     1261 ccagccagct tgaccctcag tcggccgagg aggttatcgg ggcgatcttg cgactgcgag
     1321 atgaggctgg actcggagtg gtcgttgcag agcatcgact cgagaggttc ctgcagtacg
     1381 ccgagactct gaccgtcgtc gatcgcggac ggatcgccac agggtcgccc ggcaccgtgc
     1441 tggagaagtc ctcaggaggc ccccccatcg ttgtccttgg acgtgccatg ggttggagac
     1501 cgctgcctct ggggcttcgc gatgcaagac ggttagtcaa ctcgcgtcaa aggcccacga
     1561 ggccgttgcc ttctgtcgca ccggctcccc cgccaggggg cccgatgatc tcgctcgatt
     1621 ccttgactgt gaccgttgcc ggtagacctg tcgtcagaga tgtcgacctc gctgttgcag
     1681 aaggcgagac ggtcgctttg atggggcgca acggatcggg caagtcgacc ctgttgcgcg
     1741 cactgacggg ggcatacaag catcatcgtg gcaggatcgt cgggacccgg agtatcggat
     1801 acctccccca ggatccggcc agcgttctct tcagaaggag cgttgccgag gagttgggtt
     1861 cggccctttc gacccgcgac ccgcgaacga tcgaggaggc tgcccgactc ttcgggatcg
     1921 aagaacacct tgcaagttat ccccgggacc tgtcgggcgg tcagagaacc cgggtggcca
     1981 tcgcatcaac tgcatgcatg tggcctcggc tacttcttct cgacgagccg actcgaggtc
     2041 ttgacgatga gggcaaacgg tacctgcagt ggctcaccga aacctggcgc gatcgtggcg
     2101 gagcggtggt cctggcgacg cacgatgtcg agttggccgc actcgtcgct acaagagtga
     2161 tccttctgtc cgaaggtcag gtcatcgtcg atggaacgcc aacacaggtc ctcgccgact
     2221 ccctcacctt ctcgacacag atgaacaaga tcttcgagga ccccaacgtc ctaacaatca
     2281 ccgatgcact tgcagccatg cgacagtgaa acgactcccc ttcatcgcgc tcaacgtcgc
     2341 tgggcttgtg gcctttgcac ttcctttcct tcgttcagca tccactcgcg cgatcggctc
     2401 ggcccggacc gatgatgctc cttggctcgc ggccgctctg gtgccgatgc tc
//
LOCUS       DATDOZ010000159         7517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1710618, whole genome shotgun sequence.
ACCESSION   DATDOZ010000159 DATDOZ010000000
VERSION     DATDOZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710618"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VI541_03060"
     CDS             complement(<1..654)
                     /locus_tag="VI541_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLF69918.1"
                     /translation="MSAIWVMTLGAVIVESLLAHVVLLGLVLRPAFLRKSLLVLISFA
                     AGALLGDAFLHLIPEISRSPRGFDLRASLSLLAGVLAFFILEKVLHWHHSHVPHEEVL
                     HPVAVSNLVGNFLHNFVDGAIIAGSFLVSPKLGIATTAAIALHEIPQEMGDFSILVHA
                     GMRPWRAFVFNSLTALTALLGAAVTLIISSAGVAGIADVMVPFTAGGFVYIASTDLIP
                     "
     gene            complement(651..1037)
                     /locus_tag="VI541_03065"
     CDS             complement(651..1037)
                     /locus_tag="VI541_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HLF69919.1"
                     /translation="MRSTRQRQEIIRALTEAGRFLTVRELHERLGRGGPRVGLATVYR
                     AVRTLSRSGDVDVLRSGGEASYRLCAQDRHHHHLVCRDCGRAVEVVSPQIESWAKSMG
                     RRHRFTDVSHEVELFGRCSDCRGGGQ"
     gene            1139..1456
                     /locus_tag="VI541_03070"
     CDS             1139..1456
                     /locus_tag="VI541_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69920.1"
                     /translation="MGRAKLWFLGALLALVTVGCSGAPNRVGGPGIDLTPAQSVTGYI
                     TQMEFGSLHFRVDDQRQLIFDISGGYPAEDYLRVRLDSHRHLRVDYKVEQSRLVPINV
                     RDL"
     gene            1512..1898
                     /gene="sdhC"
                     /locus_tag="VI541_03075"
     CDS             1512..1898
                     /gene="sdhC"
                     /locus_tag="VI541_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HLF69921.1"
                     /translation="MSRQVQPYRRTLYRGGSSMWSWIFHRGSGLAVLGFLFLHILDTS
                     LVGLGPEHYDKFVKIYKGPVFRPLEVILMGFVIFHSFNGLRIILVDFWSKGARHQAAL
                     SRAVAVISILLFLPAAFFMLQPLFVR"
     gene            1895..2311
                     /locus_tag="VI541_03080"
     CDS             1895..2311
                     /locus_tag="VI541_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HLF69922.1"
                     /translation="MTTVSEAYEKRFSPKSAPSNWEVWSWFFMRISGLVLVFLLLGHM
                     AIMHVFGGGVDRVNYKFVALRWKNVFWRSYDFALLALGLFHGANGARVVIEDYIRKDS
                     LRVALKAFLYTVTFIFFAIGSLVIFTFTASQMPGGK"
     gene            2311..4059
                     /gene="sdhA"
                     /locus_tag="VI541_03085"
     CDS             2311..4059
                     /gene="sdhA"
                     /locus_tag="VI541_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HLF69923.1"
                     /translation="MATYHRYDSVIVGAGGAGLRAAIEASKRSKTAVISKMYPTRSHT
                     GAAQGGMCAALGNVEEDNYEWHTFDTVKGGDYLVDQDAAETMCREAIDAVIDLEHMGL
                     PFNRTPEGKIDQRRFGGHTRNHGEGPVRRACYAADRTGHMILQTLFQNCLKQGVQFFS
                     EWYVLDIIISEVPGHGPRCTGVIAYELKTGELHVFHAKSVLFATGGFGKFFKITSNAH
                     TLTGDGPGMVLRKGLPLEDMEFFQFHPTGIYGIGILLSEAARGEGGILINSDGERFME
                     RYAPTIKDLAPRDMVSRAIHQEIKDGRGINGKDYVYLDLTHLGKEVIETKLPDITEFA
                     RIYLGVEPLTEPIPIQPTAHYAMGGIPSNIEAEVVIDEDNTPLPGFYAAGECACVSVH
                     GANRLGTNSLLDIVVFGRRGGIKMAEYAASVDHIDLPEEPERQTQERLRDLLQRPKGN
                     RIAPLRKRLQEEMMDKCGVVRTEESLTEAVDMVRELRHQERFTSIDDHGTNYNTELLE
                     AFEFGFLIDLAEVTAVAARARTESRGGHWRDDYPTRDDGHWLKHSLAYRMPDGSIDLR
                     YKPVTITKYMPMERKY"
     gene            4059..4805
                     /locus_tag="VI541_03090"
     CDS             4059..4805
                     /locus_tag="VI541_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLF69924.1"
                     /translation="MAVSGLIFIGLLTFALLIWLIVVSDGRYFGTRGVRFVYQRSARF
                     WNLDEAPTRERSRSRSEANTTLLHHLETCLDGLRQPKVLDIATGTGRVPLLIAGIAGF
                     DGHVTGVDISRNMLARGRAIIRSEGLEDQIALVESDGADLPFDDGSFDLVCLVDAPLA
                     RPRRTLDEMVRVLTPAGRSLIAKQSDRWVLAVPGMNRGSRWITRWLTQAGMADVDVET
                     WHGGNIIITARKPREGKGQESSGLASVGPL"
     gene            4828..5466
                     /locus_tag="VI541_03095"
     CDS             4828..5466
                     /locus_tag="VI541_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HLF69925.1"
                     /translation="MGIDRIIKERHLLTHPFYARWQKGKVPIEVLREYAKQYFHYEKA
                     LPGFLRAALEHIDEGPAREAVTQVLEDESSNRPHADLWIDFATGLGVTEDEVITSSPT
                     PRTTNLIETYRSLSARGSEEALGAIYAYESQFPDVASAKADGLRVHYGVESESALRFF
                     DLHSTLDVEHGAAIRSGFNDSEFSRESAHLALDAWWGMLDQFEAMSENFTAA"
     gene            5466..6164
                     /locus_tag="VI541_03100"
     CDS             5466..6164
                     /locus_tag="VI541_03100"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HLF69926.1"
                     /translation="MRVEIHIARFDPARDEKPWWGEYVVDVEDDARVLDAIHEVKWYV
                     DGTLALRRSCAHGVCGSDAMVINGHNRLACQVLCRNIEQPIRVEPLQGLKVEKDLIVD
                     MTPFYAGYESVKPWLINDGVVPETERLQSPEERERFEDTTKCILCAACTTSCPIFWSD
                     DEYVGPAAIVNAHRFINDSRDTGSDERLEILSSKSGVFRCRTTFNCTDACPRGIKVTE
                     AIQEVKRTILFKKM"
     gene            6193..7260
                     /locus_tag="VI541_03105"
     CDS             6193..7260
                     /locus_tag="VI541_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HLF69927.1"
                     /translation="MSHRITLIPGDGIGPEVAEATRLAVEATGVSIEWDVQPAGAEVA
                     EAEGTPMPDRVIASIRKNKVALKGPVTTPVGHGFRSVNVTLRTELDLYALLRPCRHYP
                     GVRTRHPGTDLVVVRENTEDLYGGIEFEAGTRDNQKFRDYLRFFEGYRLTPDTGITVK
                     PISAYATRRIVRFAFDYAIRSGRSKVTVGHKANIMRHSDGIFLQTARETSWEYPSVRF
                     EEMQLDELAMWLVWEPQVMDVLLLPNLYGDIISDLCAGLIGGLGVAPGVNLGDEIAVF
                     EATHGSAPDIAGKGLANPAGMMLSSVMMLRHIGEIDAAHRLESALARVMEEGKYVTRD
                     LGGTSSGAEFADAVMGCLRQE"
     gene            7263..>7517
                     /locus_tag="VI541_03110"
     CDS             7263..>7517
                     /locus_tag="VI541_03110"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLF69928.1"
                     /translation="MPTEIVIVAPDKPGVVATIGEMFGEVGVNIIAGAAFTHAGQGIL
                     HFVVDAADRAIHALENAGFQVSQVREVLAVSIEDRPGEIGR"
BASE COUNT         1551 a         2292 c         2267 g         1407 t
ORIGIN      
        1 ggggatgagg tcggtgctag cgatgtaaac gaaaccgccc gcagtgaagg ggaccatgac
       61 atcggcgata ccggcgactc ctgccgagga gatgatcagg gtcacagccg cccccagcag
      121 cgctgttagg gccgtgaggg agttgaagac gaacgcgcgc cagggccgca tccccgcgtg
      181 cacgaggatg ctgaagtccc ccatctcctg agggatctcg tgaagggcga tagccgctgt
      241 ggtggcgatc ccaagcttcg gcgagaccag gaaactgccc gcgatgatcg ctccatctac
      301 gaagttgtga aggaagttcc cgactaggtt cgacacggcg acggggtgaa gaacctcctc
      361 atgtggaaca tgtgagtgat gccaatgaag caccttctct aggatgaaga aggcgaggac
      421 cccagccagc agtgagagcg aagcccgcag atcgaaaccc cgcggcgaac gcgatatctc
      481 ggggatcagg tgtaggaagg catctcccag aagggctccg gcggcgaatg agatgagaac
      541 gagcaaactc ttccgaagga aagctgggcg aagtacaaga cccagtaaga cgacatgcgc
      601 aagcagactc tcgacgatga ccgcacccag ggtcatgacc cagatggcac tcactgaccg
      661 ccacctcgac agtccgagca ccggccgaac aactcgacct catgagaaac atcggtgaag
      721 cggtgacgcc tccccatgct ctttgcccaa gactcgatct gcggcgagac gacctcgacc
      781 gctcgtccgc aatctcgaca caccagatgg tgatggtggc gatcctgcgc acataacctg
      841 tagctggctt cccctccgct tcgcaggaca tcgacatcgc cactgcgaga gagagttcta
      901 accgctcgat acaccgttgc cagacctacc cggggccccc cacggccgag ccgctcgtgc
      961 agctctcgaa ccgtgaggaa gcgtcctgcc tcggtgaggg cacggatgat ctcctgtcgc
     1021 tgcctggtgg acctcaagcc cttcgtcatg ggaaccatga gacagttatt gagaatgatt
     1081 gtcaatatca ctgggcggag gaacctaaac ccagggggtg accatggata atcgggcgat
     1141 gggaagagcc aaactctggt tcctgggcgc cctactcgcg ctggtcaccg tcgggtgttc
     1201 gggagctccg aaccgcgtcg ggggccccgg gatcgacttg acgccagcac agtccgtcac
     1261 cggatacatc actcagatgg agttcggcag tctccacttc cgggtcgacg accagcgtca
     1321 actgatcttc gacatcagcg gcgggtatcc cgccgaggac tacctccggg tgcgcctcga
     1381 ttcacatagg cacctgcgtg tcgactacaa ggtggagcag tcccgtctgg tacccataaa
     1441 cgtcagagat ctctgaggag tttcgcaagt ccatctcggt cggtcgggcc ggattgatat
     1501 attggtttct gatgagtcgc caagtccagc cctaccgacg caccctctac cgcggcggca
     1561 gcagtatgtg gtcctggatc ttccacagag gcagtggtct cgcagttctc ggcttcctct
     1621 tccttcatat cctcgacaca tcgctggttg gtctcgggcc cgagcattac gacaaattcg
     1681 tgaagatcta caaggggccc gtctttcggc ccctcgaggt catactcatg ggcttcgtca
     1741 tcttccactc gttcaacggg ctgcgcatca tcctcgttga cttctggagc aagggggccc
     1801 gccatcaggc ggcgctctcg cgggcggtcg cggtgatctc gatactgctc ttcctccccg
     1861 cagcgttctt catgctccaa cccctgttcg tacgatgacg acggtcagcg aggcctacga
     1921 gaagcgcttc tcgccgaaga gcgcgccatc gaactgggag gtctggtcct ggttcttcat
     1981 gcgtatctca gggctggttc ttgtattcct gcttctggga cacatggcga tcatgcatgt
     2041 cttcggcggg ggcgtggatc gtgtcaacta caagttcgtg gcacttcgat ggaagaacgt
     2101 tttttggcgt agctacgact tcgctctctt ggccctcgga ctcttccacg gcgcaaacgg
     2161 ggcaagggtt gtgatcgagg actacatccg gaaggactct ctccgcgtgg ccctcaaggc
     2221 cttcctttac acggtcacct tcatcttctt cgccatcggc agtttggtga tcttcacgtt
     2281 tacggcatct cagatgcccg gaggtaagtg atggccacct accaccgcta cgacagcgtg
     2341 atagttgggg cagggggggc cggactacgt gccgcgatcg aagcatcaaa gcgatcgaag
     2401 accgccgtca tcagcaagat gtacccgacc cgttcgcaca cgggggccgc acagggcggt
     2461 atgtgcgcag ctctcggcaa cgtcgaagaa gacaattacg agtggcacac cttcgacacc
     2521 gtcaaaggcg gcgactacct ggttgaccag gacgccgccg agacgatgtg ccgcgaggcc
     2581 atcgacgcgg tgatcgacct cgagcacatg ggcctgccat tcaaccggac ccccgagggc
     2641 aagatcgacc agcgccggtt tggcggccat acgcgcaacc acggcgaggg tcctgtgcga
     2701 cgagcatgct acgcggccga ccgcacgggt catatgatcc tgcagacgct cttccagaac
     2761 tgcctgaagc agggggtcca gttcttcagc gagtggtacg tgctcgacat catcatctcc
     2821 gaggttccgg gccacggccc ccgatgcacc ggtgtgatcg cttacgaact caagaccggt
     2881 gaacttcatg tcttccacgc gaagagcgtt ctgttcgcga ccgggggctt cggtaagttc
     2941 ttcaagatca cctcgaacgc ccacaccctc accggcgacg gccctggcat ggttctgcgc
     3001 aaggggcttc ccctcgagga catggagttc ttccagttcc acccgacggg catctatggg
     3061 atcggcatcc tgctctcgga agcagctcgc ggagagggcg gcatcctcat caactcggat
     3121 ggcgagcgtt tcatggagcg ttacgcgccc acgatcaagg atcttgcccc gcgcgacatg
     3181 gtctcgcgtg cgatccatca ggagatcaag gatgggcgcg gcatcaacgg caaggattac
     3241 gtctatctcg acctgaccca cctcggcaaa gaggtgatcg agacgaagct ccccgacata
     3301 accgaattcg cccgcatcta tctgggggtc gagcccctga cggaaccgat cccgatccag
     3361 ccaacagccc actacgcgat gggcgggata ccgagcaata tcgaggcaga agtagtgatc
     3421 gacgaggaca acactcccct accaggcttc tatgcagcgg gcgagtgcgc ctgcgtctcc
     3481 gtacatggcg ccaaccgact cggcacgaac tctctgctcg atatcgtcgt cttcggcaga
     3541 cgcggaggca tcaagatggc cgagtacgct gcctcggtcg atcacatcga ccttcccgag
     3601 gagcctgaaa ggcagaccca agagcgactt cgcgacctgc tccaacggcc gaagggcaac
     3661 cgtatcgctc cccttcgcaa gcgacttcaa gaggagatga tggacaagtg cggtgtcgtt
     3721 cgcaccgaag agtctctcac cgaggcagtc gacatggtcc gagaactgag gcaccaagaa
     3781 cggttcacgt ccatcgacga tcacgggacc aactacaaca ccgagttgct cgaagcattc
     3841 gaattcggat tcctcatcga cctcgccgag gtcaccgcgg ttgccgcaag ggctcggacc
     3901 gagagccgcg gcggccactg gagagacgac tacccaacgc gtgacgacgg ccactggttg
     3961 aagcactctc tcgcgtaccg catgcccgac gggagcatcg accttcgtta caaaccggtg
     4021 acgatcacga agtacatgcc catggagcgc aagtactgat ggccgtgagt ggtctcatct
     4081 tcatcggtct gctcacattc gcgcttctga tctggttgat cgtggtatct gatgggcgct
     4141 acttcggcac ccgtggggtc aggttcgtat atcagcgatc cgctcggttc tggaacctcg
     4201 acgaggcccc cactcgcgaa cgctcaaggt cgcgctctga agcgaacacc acgctcctac
     4261 accacctcga gacctgcctc gatggtctgc gccaacccaa ggttctcgat atcgcgaccg
     4321 gaacgggacg cgttccgctg ctcatcgctg gcatcgcagg attcgatggc catgtaaccg
     4381 gggtggacat atcacgcaac atgctcgcca ggggccgagc catcatccgc agtgaaggcc
     4441 tcgaagacca gatcgcactc gttgagagcg acggcgcaga cctgccattc gacgacggtt
     4501 ccttcgacct ggtatgtctt gtcgatgccc ccctggctcg gccgcggcga accctggatg
     4561 agatggtcag ggtcctgact cccgctggtc gatcgctgat cgccaagcag tccgacagat
     4621 gggttctggc cgtgccgggg atgaatcgtg gttccagatg gataactcgt tggctcactc
     4681 aagcagggat ggccgatgtc gatgtcgaaa cttggcatgg cgggaacatc atcatcacgg
     4741 cgaggaaacc gcgggaaggg aagggacagg aaagttcggg attggctagt gttggccccc
     4801 tttagggggc cagagaggga ggttggtttg gggatcgacc ggatcatcaa ggaacgtcac
     4861 ctactgacgc acccgttcta cgctcgctgg cagaagggaa aggttcccat cgaggttcta
     4921 cgcgagtacg cgaagcagta cttccactat gagaaggccc tgcctgggtt cctgcgggcc
     4981 gcacttgaac acatcgatga ggggccggct cgagaggccg tcacgcaggt tctcgaagat
     5041 gagtcgtcca accgtcccca tgccgatctc tggatcgact tcgcaaccgg ccttggtgtc
     5101 actgaggacg aggtcatcac ctcaagcccc acgccacgca ccacgaatct catcgagacc
     5161 taccgctcac tcagcgcacg cggttcggag gaagcgctgg gagccatata cgcatacgag
     5221 tcacagttcc ccgacgttgc gagcgcgaag gccgacggac tgagagtgca ctacggtgtc
     5281 gagtcggaat ccgctctgcg gttcttcgac cttcactcca cgctagatgt cgagcatggc
     5341 gcggccatca gatcgggctt caacgattcc gagttctcac gcgagtcggc acacctggcg
     5401 ctcgacgcat ggtgggggat gctcgatcag ttcgaggcca tgtcagaaaa cttcacggcc
     5461 gcctgatgcg cgtagagatc cacatcgccc ggttcgaccc cgcccgcgac gaaaaaccct
     5521 ggtgggggga gtacgtcgtg gacgtcgagg atgacgctcg cgtgctcgac gcgatccatg
     5581 aggtgaagtg gtacgtcgat ggaacactgg cgttgcgccg ttcttgtgca catggcgtct
     5641 gcggttccga cgcgatggtg atcaacggtc acaatcgcct ggcctgccag gtgctctgcc
     5701 gcaacatcga gcagccgatc cgggttgagc cactgcaggg cctcaaggtc gagaaggacc
     5761 tgatcgtcga tatgacgccc ttctacgctg gttacgagtc cgtgaagccc tggctcatca
     5821 acgacggtgt tgtgccggaa actgagcggc tgcaatctcc cgaagagcgc gagcggttcg
     5881 aggacacgac caagtgcatc ctctgcgcag cctgcacaac ttcatgcccg atattctggt
     5941 ccgacgatga gtacgttggg cccgcggcca tcgtgaatgc tcaccggttc atcaacgact
     6001 cacgcgatac gggatctgac gaacgtctcg agatcttgag tagcaagtcc ggggtgttta
     6061 gatgccggac aacgttcaac tgcaccgatg cctgcccgcg tggcatcaag gtcaccgagg
     6121 ccatccaaga agtcaaaagg accatcctct tcaagaagat gtgaagcctg gcctcggtta
     6181 ggctgacctc ttatgtcaca tcggataact ctcatccccg gagacgggat cggcccagag
     6241 gtggccgagg ccacgcgact tgccgtcgag gcaacgggag tgtccatcga gtgggatgtc
     6301 cagcccgcgg gggccgaggt cgcagaggct gaggggaccc cgatgccgga ccgagtcatc
     6361 gcctcgatcc gaaagaacaa ggtcgcgctc aaggggccgg taacgacacc agtcggacac
     6421 gggttccgaa gcgtcaacgt gacgttacgg acagaactcg acctctatgc gctgcttcgg
     6481 ccctgtcgcc actaccccgg tgtgcgcacg cgccaccccg gaaccgacct tgttgtggtt
     6541 cgcgagaaca cagaggacct gtacgggggg atcgagttcg aggcggggac ccgcgacaac
     6601 cagaagttcc gcgactacct tcgtttcttc gaagggtacc gactaacgcc cgacaccggc
     6661 atcaccgtga agcccatatc ggcctacgcc acccgaagga tcgtacgatt cgccttcgac
     6721 tacgccatcc gatctggacg tagcaaggtc accgtcggcc acaaggccaa catcatgagg
     6781 cactccgatg gcatctttct gcagaccgcc cgggaaacct cctgggagta tccgtcggtt
     6841 cgattcgaag agatgcagct ggacgagctc gccatgtggt tggtttggga accccaggtc
     6901 atggacgtgc tgctgctccc caacctttac ggcgacatca tcagcgatct ctgcgccggg
     6961 ctgatcgggg gcctgggcgt ggcccccggc gtgaaccttg gcgatgagat cgccgttttc
     7021 gaagcaacgc atggctcggc ccccgacatc gcgggcaagg gccttgccaa tccagctggg
     7081 atgatgctct catcggtgat gatgctcagg cacatcggtg agatagacgc cgctcaccgc
     7141 ctggagtcag ctttggctag agttatggag gagggtaagt acgtaacacg agatctcggc
     7201 ggtacgtcgt cgggcgccga gttcgcagat gccgtcatgg gctgcttgag acaggagtga
     7261 agatgccaac cgagatcgtc atcgttgccc cggacaagcc cggagtcgtc gcgaccatcg
     7321 gcgagatgtt cggagaggtc ggggtcaaca tcatcgcggg tgccgcattc actcatgcgg
     7381 ggcaagggat cctgcacttc gttgtcgacg ccgcagatcg ggcgatccac gccctcgaga
     7441 atgcgggctt ccaggtcagt caggtgcgcg aggttctcgc cgtctcgata gaggacaggc
     7501 cgggcgagat cggcagg
//
LOCUS       DATDOZ010000160         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1452410, whole genome shotgun sequence.
ACCESSION   DATDOZ010000160 DATDOZ010000000
VERSION     DATDOZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452410"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VI541_03115"
     CDS             <1..214
                     /locus_tag="VI541_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLF69929.1"
                     /translation="VVDGLPETKRLAGTNLAEVATHFDERSGKAFAFCAIDNLGKGAA
                     GQAIQNMNIMLGFDEGLGLSSVGLIP"
     gene            234..1403
                     /gene="argJ"
                     /locus_tag="VI541_03120"
     CDS             234..1403
                     /gene="argJ"
                     /locus_tag="VI541_03120"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HLF69930.1"
                     /translation="MSAASIFLPRGFKGAAATAGIKASGKPDLVLLMSELPATVAGVF
                     TTSSAAAAPVIVSRSRVADGKARAVVVNAGCANAATGERGLKDAIRMSEYAANALDIP
                     VDETCVCSTGLIGSFLPMLKVETGIDIAAKSLGYEDDPIATAIMTTDTRPKIASIQHP
                     DGWRVGGVAKGAGMIAPNMATMLAFITTDAKVDSALLREVMREVTAETFNSITIDGDT
                     STNDTFLCFANGASGIAPSREAFSAALRMVAGSLAEQIVADGEGATKLVRIRVRGAAE
                     DAEARSAARTVAESVLVKTALFGEDPNWGRIVAALGRSGARMDLSKMSISIGGIGVLT
                     EGQPAGDSALALARAAMKSHEFAIVADLFVGGGTAEFLTSDLSVDYVKFNADYET"
     gene            1400..>2011
                     /gene="argB"
                     /locus_tag="VI541_03125"
     CDS             1400..>2011
                     /gene="argB"
                     /locus_tag="VI541_03125"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HLF69931.1"
                     /translation="MNPSEKAHILVEALPYIRKWWGKTVVIKVGGEILNDEATLDAFV
                     TDVVMMRFVGMSPLVVHGGGPQISETMRRLGKEPVFKGGYRVTDAETIDIVKMVLLGS
                     LNKKIVTAMSSHGGKAVGVSGEDGELLMASRSLGPHGEDLGFVGEVDEVNPKIIETLI
                     ADEFIPVVAPVAAGADGTYNVNADLAAGALAGALGAQKIVFLTN"
BASE COUNT          394 a          572 c          668 g          377 t
ORIGIN      
        1 ggtcgtcgat ggtctgcccg agaccaagcg cctggccggg acgaacctcg cggaagttgc
       61 gacccacttc gatgagcgaa gcggtaaggc tttcgctttc tgtgcgatcg acaacctggg
      121 aaagggcgcc gctggtcagg cgatccagaa catgaacatc atgctgggct tcgatgaggg
      181 gctcggcttg agttcagtgg ggctcatccc gtgatgtcat tggaaggcca gcggtgagtg
      241 cggcgagcat cttcctgccc agggggttca agggggcggc tgccactgct ggcatcaagg
      301 cctccggaaa gcccgacctc gtgctactca tgtcggagct gccggcaacc gtcgccggtg
      361 tcttcacgac gagtagcgcg gcggctgccc ccgtcatcgt ctcgcgaagc agagtcgccg
      421 acggcaaagc tcgcgcggta gtcgtgaacg ctggatgcgc taatgcggcg acgggcgaga
      481 gagggctcaa ggatgcgatc aggatgagcg agtatgcggc caacgctctt gacatcccgg
      541 tggacgagac ctgcgtctgc tcgacgggtc tgatcggatc cttccttccg atgttgaaag
      601 tggagacggg gatagacatc gccgccaagt ctctcggcta tgaagacgac ccgatcgcga
      661 cagcgatcat gactaccgat acgcgcccga agatcgcatc gatccaacac cctgacggtt
      721 ggcgtgttgg gggtgtggcc aagggagccg gcatgatcgc tcccaacatg gccacgatgc
      781 tcgctttcat caccaccgat gcgaaggtgg actcagcgct gcttcgagag gtcatgcgcg
      841 aagtcaccgc agagaccttc aactccatca ccatcgatgg cgatacgagc acgaacgaca
      901 ctttcctctg cttcgccaat ggggcaagcg gtatcgcacc cagtagggag gccttctcag
      961 cggcccttcg catggtcgct ggttcgcttg cagagcagat cgtcgccgat ggtgaaggcg
     1021 cgaccaagct tgttcgtatc cgggtgaggg gggccgccga agatgcagag gcaaggtcgg
     1081 ctgcgcgaac cgttgccgag tcggtcctgg taaagacagc cctcttcggc gaggacccca
     1141 actggggccg catagtggcg gcgctcgggc gctcgggcgc gcgcatggac ctctcgaaga
     1201 tgagcatctc gatcgggggg ataggcgtcc tgaccgaggg ccagcccgcc ggagactctg
     1261 ccctggcact cgcacgtgcc gctatgaagt cgcatgagtt cgccatcgtg gcggacctgt
     1321 tcgtcggcgg gggtaccgcc gagttcctaa cgtctgacct gtcggtggac tatgtcaagt
     1381 tcaatgcgga ctacgaaaca tgaacccatc cgagaaagct cacatcctcg ttgaagcgct
     1441 cccatacatc cggaagtggt ggggcaagac cgttgtcata aaggttggcg gtgagatcct
     1501 caacgacgag gcgacgctcg acgcgttcgt gaccgatgtg gtgatgatgc gattcgtggg
     1561 gatgtcaccg cttgttgtgc atggaggggg cccccagata tccgaaacga tgagacgtct
     1621 agggaaggag cctgtcttca agggtggtta ccgcgtcaca gatgccgaga cgatcgacat
     1681 cgtgaagatg gtccttctgg gttccttgaa caagaagatc gtcactgcga tgtcgagtca
     1741 tggcggcaag gcggtgggcg tatcgggcga ggacggagag ctgctgatgg catcgcgatc
     1801 gctcggcccc catggcgagg acctcggctt cgtcggcgag gtagatgagg tgaaccccaa
     1861 gatcatcgag accttgatcg cagatgagtt catacccgtc gtggccccgg tagccgccgg
     1921 agcggatggc acttacaacg taaacgccga tctcgcggcc ggtgccttgg ccggcgcgtt
     1981 gggggcccag aagatcgtct tcttgaccaa t
//
LOCUS       DATDOZ010000161         6969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3816277, whole genome shotgun sequence.
ACCESSION   DATDOZ010000161 DATDOZ010000000
VERSION     DATDOZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816277"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2156
                     /locus_tag="VI541_03130"
     CDS             <1..2156
                     /locus_tag="VI541_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69932.1"
                     /translation="NPHGNADIFARNLPSGPVIRVTANDAHQINPDIDGTVVTWQDKR
                     NVTEPPNIDVFRKDLIGGPESIVTFSPGDQGVPVISGDTVAWRDQRRGPTKWDVYARV
                     GAGDETLVWEQPASDIPPLGTLGFAQTVALDGGRLVWDHIPDCGDPGGFPGDQCPSQL
                     LTCVLPCAGGGVPVDPDPDQGQIDPDVSGSRIVWGTTGNSFEGGAFVKDMSSGAAAAA
                     VDTALTRGGAPEIEGTLVTWNGAPCREPTCPIPTDAEQGQDIFWRDVTSAALPLRANS
                     IGFGGFEGGGSPTASGNNVVYHRRSRVYGTSLTPRDDFEVSSGLATGTGRPMIVGSRA
                     VWFEGVTEECIFNEDGPPDCTVTDVGGVFSRDPFSSGPTTQLSDPDGMGGPPDVDGTK
                     VVWRCDDTTLCVREGATITPHDISGSLVSPSSIVVDRVRLAGDTVVWGEFEQVGVDET
                     SSVRLHALHLLSDDDEILGPGPSDSEPVFNFDISGNKVVWTGRGGSDPGRSKVWLNTV
                     AGCPPGTSPCDHVADAVEFEVASESVEFGDVSIDGLRAAWDACFFDEGGGTCDIYTRL
                     LSEPGHHLVTDGSMAGAREPYVDGDRILWVYDTFISNFTTDIMMESVTGDPLPQPELV
                     PPGIVKNLKAVVDASVTLTWTNPGDSDFKGVRILRKSGNQPPSSPTDPTASILFDGLS
                     ASFIDKDVTSGQRYAYAVFTYDEFPNFSSAVTKRT"
     gene            complement(2177..3679)
                     /locus_tag="VI541_03135"
     CDS             complement(2177..3679)
                     /locus_tag="VI541_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat domain-containing M23 family
                     metallopeptidase"
                     /protein_id="HLF69933.1"
                     /translation="MKTRKYLLAARLASIALFVGAQFGHAPAQALIRPIAFPVLGPVT
                     FSNDFGAPRAGHTHQGNDIFGLKMQPLVAAVDGRITLATFPEPSWGWAIFIRDADGYE
                     YRYLHINNDTPGTDDGAGGGMNAFAPDMTAGNPVVRGQLIGWLGDSGNAESTRPHLHF
                     EIRAPDGTAINPFDSLIPAEHLAGAVVPPPLPGEILAYGQFYGGAWIAAGNFDADAGP
                     EVVTGAGPGGGPHVRTFEVSGTSFGAAHSSLLAYAPGFTGGVDVAAADVDGDGIDEIV
                     TGAGPGGGPHVRIFKSDGRAVGGFFAYDGGFRGGVRVSAADFDGDGIDEIVTGAGPGG
                     GPHVRIFKSDGTPIGGFFAYDGGFRGGVDVAAADVVTEPGASATATSQPEIVTGAGAG
                     GGPHVRIFSLDGSERGGFFAYPQGFPGGVRVAAADMNNNGRAEILTGAGPSGGPHVRL
                     LRADGSEISSRFPFNGWFNGGVDVTALPRRTFASSGPGRRASVRRGPAAP"
     gene            3990..>6969
                     /gene="rpoB"
                     /locus_tag="VI541_03140"
     CDS             3990..>6969
                     /gene="rpoB"
                     /locus_tag="VI541_03140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLF69934.1"
                     /translation="MKEEPRLASHPASRERVSFARFPEIQQLPDLISIQRDSFDWFLS
                     EGLRETLRDISPIEDFTGNLQLEFAEHRFEDPKHSVEECKEKDMTFSQSLFVTSRFMN
                     KETGEIKEQTVFMGDFPMMTPKGTFVINGTERVVVSQLVRSPGVYYGRSLDKVSDRDL
                     FDVKVIPARGAWLEFETDKKDLVAVRIDRKRKQHVTVLLKAMGMSAEEISELFAAESI
                     NATLEKDHIETVEDALMDIYRKLRPGEPPTPESARTLLDNLFFNWKRYDLAKVGRYKV
                     DKKLGPLDAKFDKVLKRMHADLVAREKPGKEAFEQDRWKAYAAIRKDEQGPRTPAYKS
                     VLTYEDILKTIDYIVKLHRGDEGFEVDDIDHFGNRRIRSVGELIQNQVRIGLSRMERV
                     VRERMTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNPLAGLTHKRRLSA
                     LGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLATYARINEFGFIETP
                     YRRVDSGKVTDKVDFLTADEEDRHVIAQANAPLDSGGRFTEDHVLVRRKGGEVDYVVP
                     SEVDYMDVSPKQIVSVGTALIPFLEHDDANRALMGANMQKQAVPLLRPEAPLIGTGLE
                     ARAAIDAGEVIVAEGNGVVEEVCADYLWIRYDDGLRKQYRLHKFNRSNQATCINQKPI
                     VDEGDKVRKGQVLADGPSTDLGELALGKNLLVAFMPWRGHNFEDAIILSERLVRDDVL
                     TSIHIEEHEVDARDTKLGAEEITRDIPNVGEEILKDLDDRGIIRVGAEVGPGDVLVGK
                     VTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGKVIDVRVFNRDDGDELPP
                     GVNQLVKVQIAQRRKIQEGDKLAGRHGNKGVISKVLPIEDMPFLADGTPVDIILNPLG
                     VPSRMNLGQVLETHLGWAAKKGWTAKIAADYKPASDGTFWVSTPVFDGAREPDVEEAL
                     ATVIADAGGKELVGADGKAML"
BASE COUNT         1373 a         2232 c         2116 g         1248 t
ORIGIN      
        1 acaacccgca cggaaacgcc gacatattcg ccaggaatct gccgagtggt ccggtgattc
       61 gcgtgaccgc caacgacgcg catcagatca accccgacat cgatggcacg gtcgtgacct
      121 ggcaggacaa acgcaacgtc actgaaccac cgaacatcga cgtattccgc aaggacctca
      181 tcgggggccc cgagtcgatc gtcacattca gtccagggga tcagggcgtg ccggtcatct
      241 ctggcgacac ggtcgcatgg cgtgatcaac gacgcgggcc aactaagtgg gatgtgtatg
      301 cgcgcgtcgg tgcgggggat gagactctcg tctgggaaca acccgcgagc gacatcccac
      361 cgctcggcac tctcggtttc gcgcagactg ttgcgctaga tggtggacga ctcgtctggg
      421 accacatccc cgactgcgga gaccccggcg gttttccggg cgaccagtgc ccttcgcagc
      481 tgctgacatg cgtactgcca tgcgcgggcg gaggcgtccc ggttgacccc gaccccgatc
      541 aaggccagat cgaccccgat gtctccggtt ccaggatcgt gtggggcacc acgggcaata
      601 gtttcgaggg cggtgccttc gtcaaggaca tgagttcggg ggccgccgcg gccgctgttg
      661 acactgccct cacccggggt ggtgcgcccg agatcgaggg cactctcgtc acttggaatg
      721 gagcaccctg tcgagaacca acctgcccga tcccgacgga cgcggagcaa gggcaagata
      781 tcttctggcg cgacgtcacg tctgctgccc tgcccctgcg ggcaaactcg atcggcttcg
      841 gaggcttcga ggggggcgga tctcctacgg cctcaggcaa taacgtcgtc tatcacagac
      901 ggagccgtgt ctatggaaca tcgcttacgc cacgcgacga cttcgaggtg agcagcgggc
      961 ttgcgaccgg caccggtagg cccatgatcg ttgggagcag ggcggtgtgg ttcgagggag
     1021 ttaccgaaga gtgtattttc aatgaagacg ggccacccga ttgcacggtg acggatgtcg
     1081 gcggggtttt ctcgcgagac ccgttctcgt cgggtcccac gacgcaactc agcgatcctg
     1141 acggtatggg gggccccccg gatgtcgatg gaaccaaggt cgtgtggcga tgtgatgaca
     1201 caacgctatg cgtgagagag ggtgcaacga tcacacccca cgacatctca gggtcccttg
     1261 tttcgccctc gagcatcgtc gtcgataggg tgcgcctcgc aggagacacg gtcgtgtggg
     1321 gtgagttcga acaagtgggg gttgacgaaa cgtcgtccgt tcgactccac gcccttcacc
     1381 ttctcagcga cgacgacgag atcctgggtc ccgggccctc cgactccgaa ccggtcttca
     1441 acttcgacat ctcgggcaac aaggttgtct ggactggccg gggcggaagt gaccccggaa
     1501 gatccaaggt ctggctgaac accgtcgccg gatgtccccc aggcacttcg ccgtgcgacc
     1561 acgtggccga tgctgtcgag ttcgaggtag cgagcgaaag cgttgagttc ggggacgtct
     1621 cgatagatgg ccttcgcgcg gcctgggacg cctgcttctt cgatgaaggg ggcggtacgt
     1681 gtgacatata cacgaggctt cttagtgagc cggggcacca tctcgtgacc gatggctcga
     1741 tggctggggc tagagagccc tacgtcgatg gagaccgcat actctgggtg tacgacacct
     1801 tcatctctaa cttcacgacg gacatcatga tggagtcggt tacgggggac ccactacctc
     1861 aaccggaact agtgccccca ggcatcgtga agaacctcaa agcggttgtg gacgcatcag
     1921 tgacgctgac ttggaccaat cctggggaca gcgatttcaa gggcgtacgg atcctgcgca
     1981 agtccgggaa tcagccaccc tcatccccga cggatccgac ggcctcgatt ctcttcgatg
     2041 gactctcggc atcgttcatc gacaaggacg tcacgtccgg acagagatac gcctacgcgg
     2101 tattcaccta cgacgagttc cccaacttct cctcagccgt aacaaagagg acctagcccg
     2161 gaggcacgtg ccggccctag ggggccgcgg gacctcgtct gaccgatgcg cgcctgcccg
     2221 gcccgctgct cgcgaaggtt cgccggggta gtgccgtgac atcaacgccc ccgttgaacc
     2281 aaccgttgaa agggaagcgg ctcgatatct cagagccgtc agcgcgcagg agcctcacgt
     2341 gcggcccgcc gcttggtccc gcacccgtca ggatctcggc tcggccgttg ttgttcatgt
     2401 cagcggccgc gacccgcaca ccacccggga atccctgcgg ataggcgaag aatccaccgc
     2461 gttcggatcc atcaaggctg aagatacgca cgtgggggcc gcctcccgcg cccgcacccg
     2521 tgactatctc gggttgtgaa gttgcagtcg cagaagcgcc cggctcagtg acgacatcag
     2581 ctgcagccac atcgactcca ccgcggaagc cgccgtcata ggcgaagaag ccgccgatag
     2641 gcgtcccatc tgatttgaag atccggacat gcggcccacc tccggggcct gccccggtga
     2701 cgatctcatc gatcccgtct ccatcgaagt ctgctgcgga taccctgacg ccaccacgga
     2761 acccgccgtc ataggcgaag aaccccccga cggctcgacc atctgatttg aagatccgga
     2821 catgtggccc acctccgggc cctgcccccg tgacgatctc atcgatcccg tcaccatcga
     2881 catcagcggc tgcaacatcg actccaccgg tgaatcccgg agcgtaggcg aggagactcg
     2941 aatgagcggc tccgaacgaa gttccggaca cctcgaaggt ccgtacatgg ggccccccgc
     3001 ccggaccggc gccggtcacc acttctggcc cggcatctgc gtcgaagttc cctgcggcta
     3061 tccacgcgcc accatagaac tgaccgtagg cgaggatctc accgggtagg ggcggaggaa
     3121 cgactgctcc cgccagatgc tcggccggga tcaaactgtc gaatgggttg atggccgtcc
     3181 catcaggcgc ccgtatctcg aagtggaggt ggggtcgtgt gctctcggcg ttcccgctgt
     3241 cgccaagcca gccgatgagc tgtccccgta ccacaggatt gcccgcagtc atgtcggggg
     3301 cgaaagcgtt catgccgccg ccagccccat cgtcggttcc gggcgtgtcg ttgttgatgt
     3361 gaaggtaccg atactcgtag ccgtcagcgt ctctgatgaa gatcgcccac ccccacgagg
     3421 gctctgggaa tgtcgcgaga gtgatgcgac cgtctaccgc ggctaccagt ggctgcatct
     3481 ttagtccgaa gatgtcgttg ccctgatgag tgtgacctgc gcggggtgcg ccgaagtcgt
     3541 tcgagaaggt caccgggccg aggacgggga aagcgatcgg ccttatcagg gcctgagccg
     3601 gtgcatggcc gaactgggcc cccacaaaga gggcaatgga tgcaaggcgc gcggcgagga
     3661 gatacttgcg ggtcttcact ctccaccttg tcggaggtca gttccctgac cttgagagtt
     3721 gccccgacag gccggttccc gcaggtcttt tcgccaccag gtacccgtga ggtacatatt
     3781 cgcgaaaaga ccggggcccc cggcccccgg ccccgaaaac cttgacagcg gctcgccata
     3841 ggcctaccct ccatagagtc gttgaatcga atacgcagtc gagactgtcc agcgcatcgc
     3901 gttgacggcc tttttcctat ctgttagtct tctctttcgt ctatccatgg ccccctggcg
     3961 ttgcaccgcg cccagggaca tctgcgacct tgaaggagga gccccgcttg gcgagtcatc
     4021 ccgcctcccg tgaacgtgtt tcattcgccc gattccccga gatccaacaa ctccccgatc
     4081 tgatttccat ccagcgagat tcattcgact ggttcctaag tgaaggtctt cgcgagactt
     4141 tgcgcgacat ctcgccgatc gaggacttca ctggcaacct gcagctcgag ttcgccgagc
     4201 atcgcttcga ggaccccaag cactccgtcg aggagtgtaa agagaaggac atgaccttct
     4261 cgcagtcgct cttcgtaacc tcccgcttca tgaacaagga gacgggcgag atcaaggagc
     4321 agacggtctt catgggggac ttcccgatga tgaccccgaa gggcaccttc gtcatcaacg
     4381 gtactgagcg cgtcgtggtc tcgcagttgg ttcggtcccc cggtgtctac tacggccgtt
     4441 ctctcgacaa ggtctcggac cgcgacctct tcgatgtcaa ggtcatcccc gctcgggggg
     4501 cctggctcga gttcgagacc gacaagaaag acctcgtcgc cgtccgaatc gaccgcaagc
     4561 gcaagcagca tgtcaccgtc cttcttaagg cgatggggat gtcggctgag gagatctccg
     4621 aactcttcgc agccgagtcg atcaatgcca cgctcgagaa ggaccacatc gagaccgtcg
     4681 aagacgcgct catggatatc taccgcaagc tgcgccccgg tgagccgccg acccccgagt
     4741 cggcccgcac actcctcgac aacctcttct tcaattggaa gcgctacgac ctggccaagg
     4801 tcggccgcta caaggtcgac aagaaactcg gccccctcga cgcgaaattc gacaaggtcc
     4861 tgaagcgtat gcacgccgac ctggtcgctc gtgagaagcc gggcaaggag gccttcgaac
     4921 aggatcgctg gaaggcctac gccgccatcc gcaaggacga gcagggcccc cgcacccccg
     4981 cctacaagtc ggtcctcacc tacgaggaca tcctcaagac catcgactac atcgtgaaac
     5041 tgcaccgcgg cgatgaaggg ttcgaggtcg atgacatcga ccacttcggc aaccggcgta
     5101 tccggtcggt cggcgagctg atccagaacc aggtgcggat cggtctctca cgaatggagc
     5161 gagtcgttcg tgaacgcatg accactcagg acgtcgaggc catcaccccg cagacactga
     5221 tcaacatccg ccccgtcgtt gcgagcatca aagagttctt cggaacatct cagctctcgc
     5281 agttcatgga tcagaccaac ccactcgcag ggttgacgca taagcgtcgc ctctcggccc
     5341 tcgggcctgg gggcctctcg cgcgagcgcg ctgggttcga ggtccgagac gtgcacccca
     5401 gccactacgg ccggatgtgt ccgatcgaaa ctcctgaagg tccgaacatc ggtctcatcg
     5461 ggagcctcgc aacttatgca cgcatcaacg agttcggctt catcgagaca ccataccgac
     5521 gcgtcgattc cggcaaggtg accgacaagg tcgatttcct caccgccgac gaagaggacc
     5581 gccatgtcat cgcacaggcg aacgccccgc tcgattccgg cggccgattc acagaagacc
     5641 acgtgttggt ccggcgcaag ggtggcgagg tcgattacgt ggttccgtcc gaggtcgact
     5701 acatggacgt ctcgccgaaa cagatcgtct cggtgggcac cgccctgatc ccattcctcg
     5761 agcatgacga tgccaaccgc gccctgatgg gcgccaacat gcagaagcag gccgtgcccc
     5821 tgctgcgacc cgaggcacca ctgatcggca ccgggctcga ggcgcgtgcc gccatcgatg
     5881 ccggcgaggt catcgtcgcc gagggcaacg gtgtcgtaga ggaggtctgc gccgactacc
     5941 tttggatccg ctacgacgac ggccttcgca agcagtaccg actccacaag ttcaatcgct
     6001 cgaaccaggc gacctgcata aaccagaagc cgatcgtcga tgagggcgat aaggtccgca
     6061 agggtcaggt cctcgccgac ggaccatcca ccgaccttgg tgaattggcg ctcggcaaga
     6121 atctgctcgt cgctttcatg ccgtggcggg gccacaactt cgaggacgcc atcatcctgt
     6181 ccgagcgtct ggtgcgtgac gacgttctaa cgtcgatcca catcgaggag catgaggtcg
     6241 atgcccgcga caccaagctg ggggccgaag agatcactcg ggacatcccc aacgtcggtg
     6301 aagagatcct caaggacctc gacgatcgcg gcatcatccg tgtgggggcc gaagtgggcc
     6361 ccggcgacgt tctcgtgggt aaggtcaccc ccaagggcga gaccgagctg acaccagaag
     6421 agcggttgct ccgggcgatc ttcggcgaga aggcccgcga ggttcgcgac acatcgttga
     6481 aggttcctca cggggagacc ggcaaggtca tcgacgttcg agtcttcaac cgcgacgacg
     6541 gcgatgaact gccacccggt gtcaaccaac tggtcaaggt gcagatcgcg cagcgacgga
     6601 agatccagga gggcgacaag ctcgccgggc gtcacgggaa caagggtgtg atctccaagg
     6661 ttctgccgat cgaggacatg ccattcctcg cggacggcac acccgttgac atcatcctca
     6721 accccctcgg cgtgccatca cggatgaacc ttggccaggt cctcgagacg catctgggct
     6781 gggcggccaa gaagggttgg accgccaaga tcgctgcgga ttacaagccg gcctctgacg
     6841 gtacgttctg ggtttcgact cccgtcttcg acggcgctcg tgagcccgac gtcgaggagg
     6901 ctctcgccac cgtgatcgct gatgcgggcg gcaaagagct cgttggtgct gacggtaagg
     6961 ccatgctct
//
LOCUS       DATDOZ010000162         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_918481, whole genome shotgun sequence.
ACCESSION   DATDOZ010000162 DATDOZ010000000
VERSION     DATDOZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918481"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="manB"
                     /locus_tag="VI541_03145"
     CDS             <1..430
                     /gene="manB"
                     /locus_tag="VI541_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576901.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HLF69935.1"
                     /translation="HSFIKQVMAQTGAIFGGEHSGHYYFRDNFRADSGLICAMFVLEA
                     LSAARAPMSTMLQPFQRYWNSGEINTRVDDQQTKMDLVAKKYPDGVVDMTDGLTIDHE
                     SWWFNLRGSNTEPLLRLNVEARDQSEGELRTNELLEAIRG"
     gene            433..624
                     /locus_tag="VI541_03150"
     CDS             433..624
                     /locus_tag="VI541_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HLF69936.1"
                     /translation="MSEQQRRFDERLLEILVCPNCHGPLSYQQEQQRLLCLGECRYEY
                     PIVDGLAQMLVSEAVKPTS"
     gene            621..1670
                     /locus_tag="VI541_03155"
     CDS             621..1670
                     /locus_tag="VI541_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HLF69937.1"
                     /translation="MNIDEAIKDAALDPQDFLGVVERFSEQFEWALDNILEPSMIDAG
                     RIDSIAILGMGGSGISGDIVRATIGPRSRLNVQTVRGYELPGWVGERTLVLAVSHSGN
                     TAETLATFDAAQRCGAQIISIASGGKLAEISGREGLPLVTFPGTYQPRASLGYLSLPQ
                     LLICERLGLLDGFTRDLNETLDLVKRRSKEWGRSAPIAENPAKQLAQALVDRLPIIYA
                     SDGPGEVAAYRFKCQLNECAKTQSYSVTFPELDHNEIVGWTGPGPRARRDSTVVVFRA
                     EGRPEMVRRVDETLSLMRDGGVDVREVMSEGRSPLARLMDLIYLGDFTATYLALATGV
                     DPTPVAIISELKSRL"
     gene            1740..2993
                     /gene="ahcY"
                     /locus_tag="VI541_03160"
     CDS             1740..2993
                     /gene="ahcY"
                     /locus_tag="VI541_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLF69938.1"
                     /translation="MPQHDVRDLALADAGRRRIEWAEGQMPVLRQIKERFELDRPFDG
                     IRMAACLHVTTETANLILTLAAGGAEVALCASNPLSTQDEIAASLVKDYEQSVYAIRG
                     IHNDGYYKHIAQTIEFKPHMTIDDGADLVSTLHTKHQDLLEAVIGGTEETTTGVVRLR
                     AMARDKALHYPIVAVNDALTKHMFDNRYGTGQSSLDGIIRSTNVLIAGRNIVVAGYGF
                     CGKGVASRASGLGANVIVTEVDPLKAIEAVMDGYRVMPMEEAAAIGDIFITVTGDRDI
                     LVQRHFEKMKDGAILANSGHFDLEIDVKALHRNAKVREVRDNVEEFRFPDSKRVYLVA
                     EGRLVNLGAAEGHPASVMDMSFANQALCLEWLVKNRGLEAGVYPVPEDIDKEVARLKL
                     ASLGVSIDSLTPEQVAYLHSWETGT"
     gene            complement(3025..>3855)
                     /locus_tag="VI541_03165"
     CDS             complement(3025..>3855)
                     /locus_tag="VI541_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLF69939.1"
                     /translation="LVNGEPVKEGLDPRKLPPRRDYLDLLCRGGYPEAINLEPDARRR
                     FFRNYVRTVTQRDVPQISRIRHVEELPRILRLLAAATSQEIKDTELAQRLGIDRRTLR
                     ANYLPLLQTVYLAHEIPAWSRNVASRVSKHPKSYLVDSGLAAHVLGTSPERLSAPTSP
                     GTGSLLETFVVNELVRQTSRFDDMGIHLFHYRAHSGGEVDLVAETDDGRVVGIEVKAS
                     TSVEGSDFLHLRAVRDRLDGLGDVEFVGGVVLYAGGAAYSFGDRLEALPIAALWLPPA
                     "
BASE COUNT          814 a         1156 c         1157 g          728 t
ORIGIN      
        1 ccattcattc atcaagcagg tgatggcgca gacgggagca atcttcggtg gcgagcactc
       61 gggccactac tactttcgtg acaacttccg tgccgattca gggctcatct gcgccatgtt
      121 cgtgttggag gcactctccg cggcgagggc cccgatgagt acgatgcttc aaccatttca
      181 gcgatactgg aactccggtg agatcaatac gcgcgtcgat gatcagcaga ccaagatgga
      241 tctcgttgcg aagaagtatc cggatggtgt cgtcgatatg actgacggtt tgacgatcga
      301 tcacgaatcg tggtggttca accttagagg ctcgaacaca gaaccccttc tgagattgaa
      361 cgtcgaggcg agggaccaga gcgagggcga gttgcgaact aacgaactgc ttgaagcgat
      421 cagggggtag agatgtcaga gcaacagagg cgattcgatg agcgtctgct cgagatccta
      481 gtctgtccca actgtcacgg ccccctctca taccagcagg aacagcagcg gctcctctgt
      541 ctgggcgagt gccgctacga gtatccgatc gtcgatggtc tcgcgcagat gttggtcagc
      601 gaggcagtga agccgacttc gtgaatatcg acgaggcgat caaggacgcg gcgctcgatc
      661 cgcaggactt cctcggcgtc gtcgagcgat tctccgaaca gttcgagtgg gcactggaca
      721 acatcctcga accctcgatg atcgacgcgg gcaggatcga ctccatcgcc atcctgggta
      781 tgggtggctc cggcatctcg ggcgacatcg tgagagcgac catcggcccc cgatcgcgtc
      841 ttaacgtcca gacggtccgt gggtatgaac tcccaggttg ggttggcgag cggaccctcg
      901 tcttggccgt atcgcactcc ggcaacaccg ccgagaccct tgcgacattc gatgcagccc
      961 aacgatgcgg cgcacagatc atctcgatcg catcaggtgg caagctggcg gagatctcgg
     1021 gtcgagaggg gctcccgctc gtcaccttcc cggggaccta ccagccccgg gcatcgctcg
     1081 ggtacttgag ccttccgcaa cttctcatat gcgaacggct cgggctgctc gacggcttca
     1141 cacgtgatct caatgagacc ctcgatctcg tgaagcgtcg ctcgaaggag tggggacgct
     1201 cggcccccat cgccgagaac ccagcgaaac agctcgcgca ggctctcgtc gaccgtctgc
     1261 ccatcatcta tgcgagcgac gggcctggcg aggtcgccgc gtaccgtttc aagtgtcagc
     1321 tgaacgagtg cgccaagacc cagtcctaca gcgtcacgtt cccagagctg gaccacaacg
     1381 agatcgtcgg atggaccgga ccaggccccc gcgcgcgccg cgactcgacc gtcgtggtct
     1441 ttcgagccga aggccgaccc gagatggtcc gcagggtcga cgagaccctg tccctcatgc
     1501 gcgatggagg tgtcgatgtc cgggaggtca tgtcggaggg acgttcaccg ctggctcggc
     1561 tgatggacct catctatctc ggtgacttca ctgccaccta ccttgccctg gcgacgggtg
     1621 tcgacccaac gccagttgcg atcatctcgg agttgaagtc aagactctga gtctcgcggg
     1681 tgccgatagt gggttaggct cataagagcg ggaacgccat caatctcaag gagcctcgag
     1741 tgccacaaca tgacgttaga gacctcgccc tcgcagacgc cggtcgcagg cgcatcgagt
     1801 gggcagaggg tcagatgcct gtgcttcgcc agatcaagga acgtttcgaa ctcgacagac
     1861 cattcgacgg gatccgcatg gctgcgtgcc ttcatgtcac tacagagacc gccaacctga
     1921 tccttacgtt ggcggcagga ggggccgagg tcgcactctg cgcctcgaac ccactctcga
     1981 cccaggacga gatcgccgct tcactggtca aggactacga acagtcggtt tacgccatca
     2041 gggggatcca caacgacggc tactacaagc acatcgccca gacgatcgag ttcaaacccc
     2101 acatgaccat cgacgacggg gccgacctcg tgtcgacact gcatacgaaa catcaggacc
     2161 tgctggaggc cgtgatcggt ggtaccgagg agacaacgac cggtgtcgtt cggctccgtg
     2221 ccatggcgcg cgacaaggct cttcactatc cgatagttgc ggtcaacgat gcactcacga
     2281 agcacatgtt cgacaaccga tacggaacgg ggcagagttc gctcgatggg atcatccgct
     2341 ccaccaacgt tttgatcgcg gggcgcaaca tcgtcgtcgc gggttacggc ttctgcggaa
     2401 agggtgttgc atcgcgagca tcagggctcg gagccaacgt aatcgtcacc gaggtcgatc
     2461 cgctcaaggc catcgaggcc gtgatggacg gctaccgcgt catgccgatg gaagaagctg
     2521 cagccatcgg cgacatcttc atcacggtca cgggcgacag agacatcctc gtgcagcgtc
     2581 acttcgagaa gatgaaagac ggcgccatac tcgccaactc gggtcacttc gatctcgaga
     2641 tcgacgtcaa ggccctgcac cgaaacgcga aggtcagaga ggtgcgtgac aatgtcgagg
     2701 agttcagatt ccccgactcg aagagggtct acctcgtggc cgagggcagg ctcgtgaacc
     2761 tcggagccgc cgaagggcac ccggcgtcgg tcatggacat gtccttcgcg aaccaggccc
     2821 tctgtctcga gtggctggtt aagaaccggg ggctcgaggc cggcgtctac ccggttccag
     2881 aagacatcga taaagaggtt gcacgactca agctcgcatc tttgggcgtc tccatcgact
     2941 cgctcacccc cgaacaggtc gcgtacctgc attcgtggga aacggggacc tgatctaaag
     3001 ggtcgacacg ccctcacgct gtagtcaggc gggagggagc cagagtgcag caatggggag
     3061 ggcctccagc cgatccccga aactgtatgc cgcacctccg gcgtagagaa cgacaccccc
     3121 cacaaactcg acatcaccaa gcccatccag ccgatcgcga acagcacgca gatgcaagaa
     3181 gtctgatcct tcgactgaag ttgacgcttt gacctcgatc ccgacgacgc gtccgtcatc
     3241 agtctcggcc acaagatcga cctctcctcc ggagtgtgct ctgtagtgaa agagatggat
     3301 ccccatgtca tcgaatcggg aggtctgtcg cacgagttcg ttgactacaa atgtctccaa
     3361 cagtgatcct gtgccgggag atgttggcgc tgagagtctc tcgggagagg ttcccagcac
     3421 atgagcagcg agccccgagt cgaccagata gctcttaggg tgtttggaca cacgggaggc
     3481 gacattgcga gaccacgcag ggatctcgtg tgccagataa acggtttgca gcagcggaag
     3541 atagttagct cgcagcgtcc ggcgatcgat tccaagacgt tgtgccagct cggtatcctt
     3601 tatctcttgg gacgtggctg ccgcgagaag cctcaggatc ctcggcaact cctcaacatg
     3661 gcgtatccga cttatctgtg gaacgtcgcg ttgggtaaca gtccgcacat agttgcgaaa
     3721 gaaccttctc ctggcgtccg gttcgaggtt gatcgcctcg gggtaccccc cacggcagag
     3781 gagatccagg tagtcgcgac gaggtggaag cttgcgtgga tcgagccctt ccttcactgg
     3841 ttcgccattg acgag
//
LOCUS       DATDOZ010000163         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_138540, whole genome shotgun sequence.
ACCESSION   DATDOZ010000163 DATDOZ010000000
VERSION     DATDOZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138540"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VI541_03170"
     CDS             <1..700
                     /locus_tag="VI541_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69940.1"
                     /translation="DVVADVDGGSTQSEEPEPPASSGCTGAIRPNRSLWYSYRASDFE
                     GLEISLSSAPAWINIYVGDSLSDLAFIGCTHNETTVGVRPGSVYHFQIAQDQVSYPVR
                     LHVASLHTGSTTVSRRYVTALGVLDDPLAAACDNNPTGPSIGTLCFDVIAYGEAQLMI
                     IDDIRIGQVEINTWFDDGAWEMSFCTTDDPGDAGRQRTRVIPPGARRFYVSIGNGSNC
                     LNPGTKGTLIVSLR"
     gene            712..>3601
                     /locus_tag="VI541_03175"
     CDS             712..>3601
                     /locus_tag="VI541_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69941.1"
                     /translation="MSKVLAIVMFAFNLPTHGAEAATQVAFVPNWRAAINDPLQASAD
                     PTDATLSSEDQSDPAEQLRGYECDKRGYEKLSSFGDEQAYFLTTATLTVDSRATYSTL
                     EKQTVPSSAEVSGTSVATPYWFDAAHYGAYTCGWVKPFQGYFIMARGTYRYDQTSPAG
                     YAMADVECATGFAPADDGRAGSDKDLSQFISAVYRQQPYDPDHPDLRYLNPSNLYGTE
                     WKPDRFLVTGTDAVRDLLDMRINGDFHNKTWRPVYPDPANPACSGDIDHTYVLDENET
                     DSGPDPTINPSIDESNTTTALHFRIADIREYTDWDNCGLIQVSIRRTSGPRLRSELEV
                     GDFRPPDWDRCSEWRPAPYRQRIEVGDGPRTAESIHQWADGQSNQKISEITSCHPDVA
                     SGIPDTRPCASVPFPPIDQNGTAHTAADLIAGNPPLRSLSDHAPYWFWPTRTAVPARS
                     GSSDDISRQVTWPFTGRLRHTVSATRVVALRTPREAPYSHIREEVLGSRNLAAVTDVI
                     DPCEQLASLDGECGRYSMASWKDSRVTPTVSESSFDIEAMMFDSHPSCLRDQEVGRPV
                     PVPEAVSGTSVAPDSGRCGSPGDTFTWAPPRLRDTSLFRTVAYRIEYTCKVIHGAGAN
                     PIPMDGSIIISDASIDAPPSTPPTPTLLPPTITVPLPDSLVPQIATLAGTVTPGSSVS
                     IYDQGVLLKATPTISSGVWQVAHAFSAQGNHLVTATATSPDGAISVASDPVSFTVDTK
                     APTVTIDVEGGSVPSIQLTQQAPLRGTASDEGGLARVLLLVIDEAKNKHILPLDCSSS
                     GQTLPGCVTGTSVTWQQIASDFLPSGKLSVRAIAIDRAGNLGQSESHDVTLIEDPTGS
                     IQGQPRDLRAAILGDSIVLSWQGVADASGYRIYRGDTPGGESLVAKASPPMDPTERVV
                     FVDDHPPAGQSYYYRVGACREATCDLDSDFHRISGEITLPFRVRPPVLK"
BASE COUNT          764 a         1110 c          987 g          740 t
ORIGIN      
        1 cgacgttgtg gccgatgttg atggagggag cacacaatcc gaagagcctg agccccctgc
       61 gtcgtcggga tgcacggggg ccatcaggcc caacaggagc ctgtggtact cctaccgcgc
      121 atctgatttc gagggtttgg agatttcact gtcatccgcc cctgcctgga tcaacatcta
      181 cgtcggcgac agtctgagcg acctcgcgtt cattggctgt acgcacaatg agacgactgt
      241 cggagttaga cctgggtctg tttatcactt tcagatcgcc caggatcagg tctcctatcc
      301 cgtccgactg catgtggcga gtttgcacac cgggtccacc actgtgagtc gtcgctacgt
      361 cacagcactg ggtgtccttg atgaccccct tgccgcggct tgcgacaaca atcccacagg
      421 cccttctatc ggcaccctct gcttcgatgt gatcgcttac ggcgaagcgc aactcatgat
      481 catcgacgac ataaggatcg ggcaggttga gatcaacaca tggttcgatg acggcgcttg
      541 ggagatgtct ttctgcacca ccgacgaccc tggcgacgcc ggaagacaac ggactcgagt
      601 gatcccccct ggagctcggc gcttctatgt ttcgatcgga aatgggtcga actgcctcaa
      661 ccctggcaca aaaggaaccc ttatcgtgag tttgagatga gaagaaggca tgtgtccaag
      721 gtcttggcga tcgtgatgtt cgccttcaac ttgccgactc acggagcgga agccgctaca
      781 caagtcgcct ttgtgccgaa ctggcgtgca gccatcaacg atcccctcca agcctccgcg
      841 gatccaacag atgcgaccct aagcagtgag gatcaatccg atcctgccga acagctgaga
      901 ggctacgagt gcgacaagcg agggtacgag aagctcagct ctttcggaga tgagcaggct
      961 tacttcctaa caacggcgac cctgacggtc gattctcgag ctacctattc gaccctcgag
     1021 aaacagaccg tgccatcttc agctgaggtt tcgggaacgt cggtcgcaac gccttactgg
     1081 ttcgacgcgg cccactacgg cgcatacacc tgcggctggg tcaagccctt tcagggttat
     1141 ttcatcatgg ccagagggac gtacagatac gatcaaacct caccggcggg ctacgcgatg
     1201 gctgacgtcg agtgtgcaac tggcttcgca cccgccgatg atggtcgggc aggatccgac
     1261 aaggatctct cacagttcat ctccgctgtc taccgccaac aaccctatga tccagaccat
     1321 ccggacctca ggtatctgaa cccttccaat ctctacggaa ccgaatggaa gcccgatcga
     1381 tttctcgtca ccggcacaga cgcagttcgc gacctgctcg acatgcgcat caacggcgac
     1441 ttccacaaca agacttggcg acccgtctac cccgacccgg ccaaccctgc atgcagcggt
     1501 gacatcgacc acacatatgt cttggacgag aatgagactg acagcgggcc tgacccaacc
     1561 atcaaccctt cgatcgatga gtccaacaca accactgccc tgcactttcg catcgccgac
     1621 atccgcgagt acacggattg ggataactgc gggttgatcc aggtgagtat caggagaaca
     1681 tcgggccccc gactacggag cgagctcgag gtcggtgatt tcaggccccc ggattgggat
     1741 cgttgctcag agtggcgccc cgcgccttat cgccaacgga tagaggtggg ggatggaccc
     1801 agaaccgctg agtcgatcca tcaatgggcg gacggtcaat cgaatcagaa gatctccgag
     1861 ataacgtcct gccaccccga cgttgcttcc gggatccctg acactcgccc atgtgcttcg
     1921 gtcccctttc ctcccatcga ccagaacggt actgcccaca ctgccgcgga tctcatcgcc
     1981 ggtaaccctc cgcttcgatc tctgagtgac cacgccccat attggttctg gcccaccagg
     2041 acggcggtac ctgcgaggtc aggttcgagc gacgacatca gccgccaagt gacatggcct
     2101 ttcacaggca gacttcggca cacagtctct gcaaccaggg tcgtcgcgct caggactcct
     2161 agggaagctc cctactcgca tatccgagaa gaggtccttg ggagtagaaa cctggcggcc
     2221 gtgactgacg tgatcgaccc atgcgaacaa ttggcgagtc ttgatggtga gtgtggtcgt
     2281 tactcgatgg cttcttggaa ggattccagg gtaactccca ccgtctccga atcgtctttc
     2341 gatatcgaag cgatgatgtt cgactcgcat ccaagttgtc ttcgagatca agaagtgggg
     2401 cggccggtgc ccgttcccga ggcggtgtca gggacgagcg tggctccaga ctcggggcgc
     2461 tgtggctcac ccggcgacac attcacatgg gcgccgccta gacttcgcga tacgtctctc
     2521 tttcgaaccg ttgcctatcg tatcgagtac acatgcaagg tgatccacgg cgcgggggcg
     2581 aatcctatcc ccatggatgg aagcatcatc atcagtgatg cttcgatcga tgctccacct
     2641 tcgactcctc cgaccccgac cctgctgccg ccgaccatca ccgtaccctt gccagattct
     2701 ctcgttcccc agatagcgac tttggcggga actgtgaccc ctggttcaag cgtcagcatc
     2761 tacgatcagg gagttctgct taaggccacg ccaacgatct cctccggcgt gtggcaggtc
     2821 gcccatgcct tctccgccca ggggaaccac ttggtcacag caacagcaac gtcgcctgac
     2881 ggagcgatct ccgtcgcatc agatcctgtc tcgttcacgg tcgacactaa ggctcccaca
     2941 gtgacgatcg acgtcgaggg tggatctgtg ccatccatcc aattgacgca gcaagctccc
     3001 ctaagaggga cggcgtcaga cgaaggtggt ctagcaaggg tcctactgct agtcatcgat
     3061 gaggcgaaga acaagcacat ccttccgttg gattgctcat cgtctggaca gacccttcca
     3121 ggttgcgtca cggggacttc agtaacttgg cagcagatag cctccgattt ccttcccagc
     3181 gggaagctat ccgtcagggc catagccata gatcgcgcag gcaatctcgg gcagagcgag
     3241 tcgcacgatg tgactctcat cgaggatccg acgggatcga tccaaggaca gcctcgcgac
     3301 ctaagggcgg ccatactggg cgattcgatc gttctgtctt ggcaaggagt cgcagatgcc
     3361 agtggctacc gaatatacag gggcgacaca ccaggcggtg aaagcttggt tgcaaaagcg
     3421 tcgcctccca tggatccgac tgaacgcgtc gtcttcgtcg atgatcatcc cccagcagga
     3481 cagagctatt actaccgagt gggcgcttgt cgagaggcta cctgcgattt ggattcggat
     3541 tttcatcgga tatcagggga gatcactctt cccttccgcg tcaggccccc ggtcctcaag
     3601 c
//
LOCUS       DATDOZ010000164         9213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3818593, whole genome shotgun sequence.
ACCESSION   DATDOZ010000164 DATDOZ010000000
VERSION     DATDOZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3818593"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VI541_03180"
     CDS             complement(<1..270)
                     /locus_tag="VI541_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018908231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HLF69942.1"
                     /translation="MAGDTAPRIGVLCLQGDFAEHARALAASAATPVDVRHRARFDEV
                     DGLVIPGGESTTIIKLLDRFDLREILTKRIESGMPVLATCAGAIIL"
     gene            complement(260..1141)
                     /gene="pdxS"
                     /locus_tag="VI541_03185"
     CDS             complement(260..1141)
                     /gene="pdxS"
                     /locus_tag="VI541_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HLF69943.1"
                     /translation="MDRGTARVKRGLAEMLKGGVIMDVTSADEAKIAEDAGAVAVMAL
                     ERVPSDIRRDGGVARMADPTKIVEIQEAVTIPVMAKSRIGHFAEAQILQSLGVDFIDE
                     SEVLTPADESFHIDKWEFTVPFVCGATGLGEALRRIGEGAAMIRSKGEAGTGNVVEAV
                     RHLRSIFDDIRALSVSPSEQLMSEAKRLGAPHELVREVAEKQKLPVVIFCAGGLATPA
                     DAALVMQLGAEGVFVGSGIFKSEDPVGRARAMVEATTYFNDPDVLAKVSRGLGEPMRG
                     IDLRDIPESQRLAPRGW"
     gene            complement(1204..2274)
                     /locus_tag="VI541_03190"
     CDS             complement(1204..2274)
                     /locus_tag="VI541_03190"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HLF69944.1"
                     /translation="MNRKLLIVLALAVLTSCSNGGRPSTDAVSQKPSPSPSPKPIICP
                     LTGREASKGIDADRPALAVKIENSTAARPQAGLDSADIVYEELAEGGITRFMAVFQCS
                     DASALGPIRSARLVDVDILLEYAPVLFAFSGANSIVRTKVDSTVGITDIRFGKHGAAY
                     HRERGRPAPHDLFSSSEKLRALSSLRGAPSSGLRFDPNASAIPTPTPARNSASPSVSA
                     TVTTPPGRQVTFDYSSSANATSYTYDPTVKAYLRFQGKVAHKLVTGRQVSAVNVIIMQ
                     VRVRQGTIRDSIGNYSPDITVVGEGKVVVLRGGVATEGRWVRESLSRRTRFVDSQGKP
                     INLLPGNTWINLVPTDRQVTVT"
     gene            complement(2318..3223)
                     /locus_tag="VI541_03195"
     CDS             complement(2318..3223)
                     /locus_tag="VI541_03195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HLF69945.1"
                     /translation="MYEQIAANRRRSALLIAFFIVLVAAVGWSVGQVTGLGVASLPVA
                     VALSLVLTWSSYYNSDKLVIRLSKAIPVDQVQWPEGQAYLGPRLRNVVEGMAIAAGLP
                     VPKTYVIYDPAPNAFATGRDPQHSAIAVTSGLLEKMDRVELEGVIGHEMSHIRNHDIL
                     VTTLAVVMAGVIALLSDWLLRGFWRMGGRRRSGGAVVGIAGLVLAILAPVIAMVIQMA
                     VSRRREFLADASGVQLTRYPPGLISALRKLKADSTVVRTGSRATAHLWIESPLDRSGA
                     GSSWLNRLFDTHPPLDERIRILESM"
     gene            complement(3230..3778)
                     /locus_tag="VI541_03200"
     CDS             complement(3230..3778)
                     /locus_tag="VI541_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLF69946.1"
                     /translation="MSGWIFVALGALIVLWAVGTYNRMRVVGNRIDNAWGQIDVQLKR
                     RYDLIPNLVESVKGYAAHEKGTFDAVTKARSMGIEAKSVPEQATAENMITQALRQVFA
                     VAEAYPQLKANENFLQLQEELTATEGKIAFARQFYNDTVLRFNNLISVFPNLLIAKSM
                     GLSKKEYFEVDEDSKAPVKVQF"
     gene            complement(3780..4853)
                     /locus_tag="VI541_03205"
     CDS             complement(3780..4853)
                     /locus_tag="VI541_03205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF69947.1"
                     /translation="MKVAMVCPYDWSFPGGVRTHIVGLSDALRGKGLSVDIFAPASGA
                     EPGVHVVGRSFPIRFNGSVARICFSRSARSKLAVALASGAYDVIHLHEPAIPSVSFLA
                     LGVARGPMVATFHASSTSSRAYSVAGPWLNRKLSKVDIRIAVSSASRDLASTNLPGEY
                     ELIPNGVNVEQFARAKADASLAALKPFILFVGRNEPRKGLQVALSAVERMRERSSVNL
                     VAVGPLPTEVPSWVRALGSVGQQTLPSIYAAADVFCAPSVSGESFGIVLAEAMAAGLP
                     VVCSDLPGYVEAAGGAALHAPVGDINETARLLERALEAETSRDLIARGRVRARELDWD
                     TLCEKVVERYQRASAGTNKRKES"
     gene            complement(4850..5770)
                     /locus_tag="VI541_03210"
     CDS             complement(4850..5770)
                     /locus_tag="VI541_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69948.1"
                     /translation="MNRTSPFAEGLTRALIYFAFRAAAWLSEHLPISWGDGVAHLAAR
                     ISFRLSRKRAIVSRNLERVLGRKPDDRLLRRAFLSYAEYWLETFRLGRYSKEQLAEMV
                     TIDDESLEDLERAFAEGRGIVVVTAHFGFYDLGVAFIGSRGWPFTAVGEVLRPRALFE
                     WFARIRVERGMKIIPSRPGAEAMAASMDTVRRGEAIALVCDRDLGKRGVPTVFFGEET
                     TLPPGPGTILTRTGAALVLAGVPKVGHRRYKMNFRRIPLPADLGSDEGASVVAITHTI
                     ARELEQLVLTDPSQWHLFMPNWPSDDPRGT"
     gene            complement(5767..6333)
                     /locus_tag="VI541_03215"
     CDS             complement(5767..6333)
                     /locus_tag="VI541_03215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLF69949.1"
                     /translation="MLRSTVQRITLPIGKGIAAVGLTPNAVTVIGSIVTLYAGWLISQ
                     GNYRTGGVMALVAGVFDSLDGAVARARGGSQRGAFLDSTLDRVSDAVLFAAVIWASLD
                     TPAPVALAMAAMVLGFMTSYIRARAEGLGLECRVGLAERAERVIILAIGLITGFVTQS
                     LVILVILSSFTVIQRFVHVWRQARADGT"
     gene            6394..8484
                     /gene="fusA"
                     /locus_tag="VI541_03220"
     CDS             6394..8484
                     /gene="fusA"
                     /locus_tag="VI541_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLF69950.1"
                     /translation="MKTYSPEKIRNVLLFGHQGAGKTTLAESMLFLAGAINRMGNIAD
                     GTTTCDFEPEEIKKTLSVSLSLAPMEWNDHKINLLDSPGYADFLGEAQAAMRVADLAV
                     LVVSAVDGVQVQHEVLWQMASKHQLPRLVFINKLDRERADFHQVLAQLSERLGPGFAP
                     VDLPLGEEAEFHGIADVIDEKAYRYDSSGKPVGEEEADGALLAELHALHVKILESVAE
                     TDDTLIEKYLNGEELTEKEVNTGLHQGLLQGSVFPVLVGSATKLIGIDRLADLIVEMG
                     PAPLEHPQIHGKTPSGEEVTRPPAVDQPTSAFVFKTVSDPYVGRISLFRVMTGSVRPD
                     STLLNASTGTDERIAQVFALRGKTQEVLPEVVTGDIGAIAKLTATHSGNTLCDRSSPI
                     IYDELEPVDRVLAKAIAPKTKGDEDKLMTGLARLQEEDPTLLVERNPETHQTLLWGTG
                     DAHLEVILDRLSRKFGVEVEELPLRIPYRETIRKPGKAVGRHVKQSGGHGQYAICNLE
                     IEPLPRGTGFEFVDKIFGGSVPNQFIPSVQKGVEKTLSEGAVAGYPVVDVKVVLVDGK
                     FHAVDSSDMAFQIAGAIGFREAVHDAGVTLLEPIMDLEVLVPDRFLGDIMGDLNSKRG
                     RIQGTDAVGNRQLVRAKVPMSEVTRYAIDLRSMTGGQGTFSSTFSHYEEVPSQIADSI
                     VAEANAAKAEKSHR"
     gene            complement(8486..8956)
                     /locus_tag="VI541_03225"
     CDS             complement(8486..8956)
                     /locus_tag="VI541_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HLF69951.1"
                     /translation="MERLFRSRMGYVGGPRLEGCFLCDLAAADAIEMPVLVRRGTAFL
                     ILNAFPYNSGHLMASPVRHCGELEDLTQQEMSDVMGLVVSAIRGLKEAFAPDGFNIGA
                     NLGEAAGAGLPGHAHMHIVPRWVGDTNFMSTVGGAKVLPESLEETYQRLLPLLR"
     gene            complement(8956..>9213)
                     /locus_tag="VI541_03230"
     CDS             complement(8956..>9213)
                     /locus_tag="VI541_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HLF69952.1"
                     /translation="AATLRASGIRVEIDASDESLGNRVRKAQVSKVPYMVVVGDKEAE
                     AATLSVRPRGGEERRGVSLDEFIADITAEIADRGSPEQSRG"
BASE COUNT         1933 a         2849 c         2706 g         1725 t
ORIGIN      
        1 aaggatgatc gccccagcgc atgtggcgag gacgggcatc ccagactcga tccgctttgt
       61 aaggatctct cttagatcga acctgtcgag aagtttgatg atcgtggtcg actcgcctcc
      121 gggtatgacc aggccatcga cctcatcgaa cctggcccga tgcctgacat ccactggggt
      181 cgccgccgag gccgccaggg cacgcgcgtg ttcggcgaaa tccccttgca aacagaggac
      241 ccctatgcgg ggagcggtgt caccagccac gcggtgcgag tcgctgcgac tccgggatat
      301 cgcgcaggtc gataccgcgc atcggttctc ccaagcctct tgataccttc gcaagaacgt
      361 cagggtcgtt gaagtaggtg gttgcttcga ccatcgcacg ggcgcggccg acgggatcct
      421 cactcttgaa tatccccgaa cccacgaaaa ctccctcggc ccccaactgc atgaccagtg
      481 ccgcatcagc aggagtggcc aaaccgccag cacagaagat gacgacgggg agcttctgct
      541 tctcggcgac ctcgcgtact aattcgtgag gggctcccaa acgcttcgcc tccgacatca
      601 actgttcaga tggagatacc gatagggcgc ggatgtcatc gaagatcgat cgcagatgtc
      661 tcaccgcctc aacgacgtta cccgtgcctg cttcgccctt cgagcggatc atggccgccc
      721 cctcgccgat acgccgcagg gcctcgccga gccccgtcgc cccgcatacg aatggaaccg
      781 tgaactccca cttgtcgatg tggaagctct catcggccgg ggtcaggacc tctgactcat
      841 cgatgaagtc cacccctagc gattgcagga tctgagcttc agcgaagtga ccgatcctgc
      901 tcttcgccat caccgggatg gtgacggcct cctggatctc gacgatcttt gtcgggtcag
      961 ccatccgggc caccccgcca tcccgccgga tatcggaggg cacacgctcg agggccatga
     1021 cggcaactgc tcctgcgtct tcagcgatct tggcctcgtc tgccgaggtc acgtccatga
     1081 tcacgccgcc cttgagcatc tcagcaagtc cccgcttcac gcgggccgtt cctcggtcga
     1141 tcaattcgca cctctgtcgt cgggaacctc gattgtagcg tgcccgagcg cgaggctatc
     1201 ttctcaggtg accgtaactt gccggtccgt tgggaccagg ttgatccatg tgttgcccgg
     1261 taggaggttg atgggcttgc cttgtgagtc aacgaaccga gtcctgcgcg aaaggctctc
     1321 tcgaacccac ctgccctcgg tcgcaacgcc cccacggaga accacgacct tcccctcacc
     1381 gacgacggtg atgtctggcg agtagttgcc gatggagtcc ctgatggtgc cctgcctgac
     1441 cctgacttgc atgatgatga cgtttaccgc gctcacctga cgaccggtaa caagtttgtg
     1501 agccaccttt ccctggaacc tgagatatgc cttcacagtg gggtcatatg tgtaggaggt
     1561 ggcgttagct gaacttgagt aatcgaaggt cacctgcctg cccggtggcg tggtcacggt
     1621 cgccgaaacg gaaggcgaag cgctattcct cgcaggtgtt ggtgttggga tggctgaagc
     1681 attcggatcg aaccgcagac ctgagctcgg cgctccgcgc agactcgaca gagcgcggag
     1741 tttctcactg gaactgaaga gatcgtgggg agccggacgt cctcgttcac ggtggtaagc
     1801 cgcaccgtgt ttgccgaatc tgatatctgt gatccccacg gtagagtcaa ccttcgttcg
     1861 aacgatggag ttcgcccctg agaacgcgaa caacactggc gcgtactcca gaaggatgtc
     1921 cacatccacc aaacgagcgc tcctgatcgg acccagggcc gatgcatccg aacattggaa
     1981 caccgccatg aatcgagtga tgccgccttc agccaactct tcatagacga tgtcggctga
     2041 gtcgagcccg gcttgaggtc gggccgcggt tgagttctcg atcttcaccg caagcgccgg
     2101 acggtcagcg tcgatcccct tcgaggcttc cctacccgtc agggggcaga tgatcggctt
     2161 cggcgatggt gacggtgagg gcttttgtga aacggcatcc gtgctcggcc gcccgccgtt
     2221 cgagcacgat gtcagaacgg caagtgccag gacgatcaga agttttctat tcaacatgct
     2281 ggggaggcta gcacgggcat catgacccga tgccggatta catcgactcg agtatccgga
     2341 tacgttcgtc gagtggaggg tgagtgtcga agagacggtt caaccaggac gaaccggccc
     2401 ctgatctatc gagaggcgac tcgatccaga gatgtgccgt cgcacgactg ccggttctga
     2461 cgacggtcga gtctgctttc agtttcctca atgcgcttat gaggccgggc gggtaccgcg
     2521 tcaactgaac gcctgacgca tcggcgagaa actcgcggcg gcgcgagacg gccatctgga
     2581 tgaccatcgc gatgaccgga gcgagtatcg ccaggaccag accggcgata ccaacaactg
     2641 cgcctccact gcggcgtctg ccgcccatcc tccagaaacc tcgaagcagc caatctgaga
     2701 gaagcgcgat gacccccgcc atgaccaccg cgagggtggt gaccaggatg tcgtggttcc
     2761 ggatatggct catctcgtgt ccgatcacac cctcgagttc cacccgatcc atcttctcga
     2821 gaaggcccga tgtcacggct atggcggagt gctggggatc ccggcctgtt gcgaaggcgt
     2881 tgggggccgg atcgtagatg acgtaggtct tcggcaccgg gagcccggcc gctatggcca
     2941 tgccctcgac cacgttccgc agccgggggc cgagatatgc ctgcccctcg ggccattgga
     3001 cctgatcaac ggggatggcc ttgctcaaac ggatcaccaa cttgtcggag ttgtagtacg
     3061 acgaccaagt aagcaccagt gacagagcga ccgctaccgg aagactcgct actccgagac
     3121 cggtaacctg gccaaccgac caaccgaccg ccgcaacaag gacgatgaag aacgcgatca
     3181 gcagcgctga tctgcgtcga ttggctgcga tctgctcata catctggagt tagaactgaa
     3241 ccttcaccgg cgctttcgag tcctcatcta cctcgaagta ttccttcttc gatagaccca
     3301 tcgacttagc gatcagcaga ttggggaaga cggagatgag attgttaaat cgcagaaccg
     3361 tatcgttgta gaactgtcgg gcgaatgcga tcttcccttc ggtcgcggtg agctcctctt
     3421 ggagttgaag gaagttttcg ttggccttca actgcgggta agcctcggcg acggcgaaaa
     3481 cctggcgcag ggcctgcgtg atcatgttct cagctgtggc ctgttccggg acgctcttgg
     3541 cctcgatgcc catggagcgc gccttggtta ccgcgtcgaa tgttcccttc tcgtgagcgg
     3601 catacccttt cactgactca accaggttgg ggatgaggtc gtaccgacgc ttcaattgaa
     3661 cgtcgatctg accccacgcg ttatcgatgc ggttccccac aacccgcatc cggttatatg
     3721 tgccaacggc ccacagaact atcaaggccc ctagggctac gaagatccaa ccagacatgt
     3781 catgactcct tacgcttgtt tgtccccgct gatgctcgtt ggtaacgctc gacaaccttc
     3841 tcgcaaagcg tatcccaatc caactcccgt gctctcacac gtccacgagc gatgagatcc
     3901 cgcgaggtct ccgcctcgag cgctcgctcc agcagtcgcg cggtctcgtt gatatcaccg
     3961 acaggcgcgt ggagtgcagc acctcccgct gcctcgacgt atccgggaag gtcagagcag
     4021 accaccggta gaccggctgc catcgcctca gccagaacga tcccgaatga ctcacccgag
     4081 accgaggggg cgcagaagac atctgcggcg gcgtagatcg agggcagggt ctgctgacct
     4141 acgctcccga gagcccgtac ccaactggga acctccgttg gcaacggtcc aaccgcaacg
     4201 aggttgaccg acgacctctc tctcatcctc tcgaccgccg acaatgctac ctggagaccc
     4261 ttgcgcggtt cattacgacc cacgaagagg atgaagggct tgagcgcggc aagggaagcg
     4321 tccgccttgg cacgcgcgaa ctgctcgacg ttcactccat tggggatgag ttcgtactca
     4381 ccgggaaggt tggttgaggc gagatcgcgt gaggcactcg agaccgcgat cctgatatcg
     4441 accttcgaaa gtttccgatt cagccagggt ccggcgaccg agtacgcgcg cgacgaggtt
     4501 gagctggcat ggaacgttgc gaccatgggc cctctcgcaa ccccgagggc gagaaacgac
     4561 acactaggga tagccggttc atggagatgg atcacgtcgt aggcccccga tgcgagggca
     4621 acggccaact tcgaacgcgc tgaccgggag aaacagatcc tcgcgacaga gccgttgaac
     4681 cggatcggaa aggaacgccc caccacgtgt acccccggtt cggcccccga cgcgggcgca
     4741 aagatatcga cgctgagccc cttcccccgg agggcatccg acaacccaac gatgtgggtc
     4801 cgaacgccac cggggaagga ccagtcgtag ggacagacca ttgcgacctt catgtgcctc
     4861 gggggtcatc cgatggccaa ttgggcatga acaggtgcca ctgcgaagga tcggtcagaa
     4921 caagctgctc gagctcgcgg gcgatggtgt gagtgatcgc aacaaccgag gccccctcat
     4981 cacttcccag atcagctggt agcgggatgc gacggaagtt catcttgtat cttctgtgac
     5041 ctaccttggg gacccccgca agcacgagcg cggcccccgt tcttgtcagg atcgttccag
     5101 gccctggggg cagggtggtt tcctctccaa agaagaccgt tggcacgcct ctcttgccaa
     5161 gatcacgatc gcagaccaga gcgatcgcct cgccacgccg gaccgtgtcc atgctggccg
     5221 ccatggcctc ggcccccggc ctcgatggga tgatcttcat cccgcgctct accctgatcc
     5281 ttgcgaacca ctcgaataat gcgcgaggcc gaagaacctc gccgacggcg gtgaaaggcc
     5341 accccctgct tccgatgaac gcgacaccca gatcgtagaa gccaaagtgg gcggtgacga
     5401 caactatccc ccgaccctcg gcgaaagccc tctccaaatc ttcgagtgac tcgtcatcga
     5461 tcgtgaccat ctcggccaac tgctctttgg agtaccggcc gagacgaaag gtctcgagcc
     5521 agtactccgc gtatgagagg aaggctcttc ggagcagacg atcgtcgggt ttgcggccca
     5581 aaaccctttc gaggttgcgc gagacgatcg cacgcttgcg ggaaagacgg aaggagatcc
     5641 gagcagcgag gtgagcaaca ccgtctcccc aggagatcgg aagatgctcg gataaccagg
     5701 cagccgcgcg aaatgcgaag tagatgagtg ctctagtgag accttccgcg aagggactgg
     5761 ttctattcag gttccatcag cccttgcctg acgccataca tgaacgaagc gctggatgac
     5821 cgtgaacgag gacaagatca cgaggataac gagagattgt gtgacgaagc cagtgataag
     5881 ccctatcgcc aggatgatca ctctttcggc ccgctccgcg agtccgacgc ggcattcgag
     5941 cccgagtccc tcggcccgcg cccttatgta ggaggtcatg aatccgagga ccatcgcagc
     6001 catagctaga gcaaccgggg cgggcgtgtc cagcgacgcc cagatcactg ccgcgaagag
     6061 cacagcgtcg gagaccctgt cgagtgtcga gtcgaggaag gcgccacgct gactcccccc
     6121 tcgcgccctt gccaccgcgc catccagtga gtcgaatacg ccagcgacaa gggccatcac
     6181 gcccccggta cggtagttgc cctgcgatat gagccaccct gcatagagag tcactatgga
     6241 accgatgacg gtgacagcgt taggggtcag gccgacggca gcgataccct tgccgatcgg
     6301 cagggtgatc ctctgtacgg tcgaacgaag caatcggctc agcattatca gggtctgtct
     6361 gtggcggtcg ggcgactata tagaatgcct cgcatgaaaa cctactctcc cgagaagatc
     6421 cgcaacgtcc tattattcgg tcaccagggg gctggcaaga ccaccctggc cgagtcgatg
     6481 ctctttctcg ctggcgcgat caaccggatg ggaaacatag ccgacggcac gaccacctgc
     6541 gacttcgagc ccgaagagat caagaagacc ttgtcggtct cgctatcact ggcgccgatg
     6601 gagtggaacg accacaagat caaccttctc gactcgccag gctacgccga cttcctgggt
     6661 gaggcccagg cggcgatgcg tgttgccgac ctcgcggtgc tagttgtgag cgcagtcgat
     6721 ggggtccagg tgcagcacga ggttctttgg cagatggcat cgaagcatca actcccacga
     6781 ctcgtcttca tcaacaaact cgaccgcgaa cgcgccgatt tccatcaagt gctcgctcaa
     6841 ctatccgagc gtcttggccc agggttcgct cccgtggatc tgcctctggg tgaggaagcc
     6901 gagttccatg ggatagcaga cgtcatcgat gagaaggcct atcggtacga cagcagcggc
     6961 aagccagtcg gcgaggaaga ggcggacggc gccctactgg cggaactaca cgcgctccac
     7021 gtgaagatcc tcgaatcggt tgcggagaca gatgacacgc tcatcgagaa gtatctgaac
     7081 ggcgaagaac tcacagagaa agaagtgaac actggtctac accaaggcct cttgcagggc
     7141 tccgtcttcc cggtgctcgt cggttccgca acgaagctca tcgggatcga caggctagcc
     7201 gacctcatcg tcgagatggg accggctccg ttggagcacc ctcagatcca cggcaagaca
     7261 ccatcgggcg aagaggtgac tcgtccaccg gcagtggatc aaccaacttc ggcattcgtt
     7321 ttcaagaccg tatccgaccc ttacgttggg aggatctcac tattcagggt gatgactggt
     7381 tccgtacgtc ccgactcaac gctgcttaac gccagcaccg gaacggacga gcgcatcgct
     7441 caggtcttcg cccttcgcgg aaaaactcag gaggtccttc cagaggtagt cacgggagac
     7501 atcggagcca tcgccaaact gaccgcaacg catagcggaa acacgctttg tgacaggtcc
     7561 tctccgatca tctatgacga actcgagccg gtggaccgtg tactcgccaa agcgatcgcc
     7621 ccgaagacga agggcgatga agacaagctc atgacgggtc tagctcggct tcaggaagag
     7681 gaccccacgc tcctggtcga acggaatccc gaaacccatc aaaccctgct gtggggcacg
     7741 ggtgacgcgc atctcgaagt catcctcgat cggttgagca ggaagttcgg ggttgaggtc
     7801 gaggaacttc cacttcggat cccctatcga gagacgatcc gtaaaccagg caaggcagtc
     7861 ggacggcacg tcaagcagtc tgggggccat ggtcaatacg cgatatgcaa tctcgagatc
     7921 gagccactac cacggggcac cggattcgag ttcgtggaca agatcttcgg tggttcggtt
     7981 cccaaccagt tcatcccctc cgtgcagaag ggcgtcgaga agaccttgtc cgagggggcc
     8041 gtcgcgggct acccggtcgt ggacgtcaag gttgtacttg tcgatgggaa gttccacgct
     8101 gtggactctt cggacatggc cttccagatc gccggcgcca tcggattccg ggaagccgtg
     8161 catgacgcag gagtcacact gctcgaaccg ataatggacc tcgaggttct ggtgccggat
     8221 cgattcctcg gagacatcat gggcgacctc aactcgaagc gcggccgcat ccaggggacg
     8281 gatgcagtag gcaaccgtca attggtgagg gcgaaggttc ccatgtcaga ggtgacacgt
     8341 tacgcgatcg acctgcgatc gatgaccggc ggacagggaa ctttctcttc gaccttctca
     8401 cactacgaag aggtccccag tcagatcgcc gacagcatcg ttgcagaagc aaacgctgcg
     8461 aaggccgaga agtcgcacag gtgattcacc gaaggagcgg tagtagtcgc tggtaggtct
     8521 cttccaaaga ttccggcaat accttggctc ctcccacagt cgacatgaag ttggtgtcac
     8581 ctacccaccg tggcacgatg tgcatgtgcg cgtgaccggg cagtccggcc cccgcagcct
     8641 cgcccaggtt cgctccgatg ttgaatccat ccggagcgaa ggcttctttc agtccacgga
     8701 tcgccgagac caccaagccc ataacatcac tcatctcctg ctgcgtcaga tcctcgagtt
     8761 cgccgcagtg gcgcaccggc gaggccatga gatgtcctga gttgtatgga aaagcgttga
     8821 ggatgaggaa ggccgtgccc cggcgaacca ggaccggcat ctcgatcgca tcggcggcag
     8881 cgaggtcgca gaggaaacat ccttcgagac gggggccgcc cacataaccc atccgggagc
     8941 ggaagagtct ctccactaac cccgcgactg ttccggcgac ccccggtcgg cgatctcggc
     9001 ggtgatatct gcgatgaact cgtcgagcga tacgccgcgt cgttcttctc caccgcgagg
     9061 acgcaccgag agggttgctg cctccgcctc tttgtcacca acgacgacca tgtatgggac
     9121 cttcgatacc tgcgccttac gaacgcgatt gccgagactc tcgtcggacg cgtcgatctc
     9181 gacccttatg cccgaagctc gcagcgtggc agc
//
LOCUS       DATDOZ010000165         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_139514, whole genome shotgun sequence.
ACCESSION   DATDOZ010000165 DATDOZ010000000
VERSION     DATDOZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139514"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="coaBC"
                     /locus_tag="VI541_03235"
     CDS             complement(<1..677)
                     /gene="coaBC"
                     /locus_tag="VI541_03235"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLF69953.1"
                     /translation="MDLAAVSGKQIALGVCGGIAAYKVVELARTLTLAGADVRVVMSP
                     SALNFVGEITFSTLTGNPVRTELFPPEAPSQIPHTDLGRTSDLVVIAPATAKTMAKYA
                     QGISEDLISALLLSASCPIIMAPAMHTEMWEDPATRENVGVLRARGVRFVGPASGALA
                     GPDVGVGRLADVADIVAAIADEVSRKEDLTGLRVLVTSGGTREAIDAVRYLGNRSSGK
                     MGTELAF"
     gene            complement(685..939)
                     /gene="rpoZ"
                     /locus_tag="VI541_03240"
     CDS             complement(685..939)
                     /gene="rpoZ"
                     /locus_tag="VI541_03240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLF69954.1"
                     /translation="MIYPKIEDLTDKVDSLYTLVVLAAKRARQINAYYSQLGEGLRDF
                     VPPQISRLGENAKALSVALEEIAQGKFGYDRPPEVEQRVK"
     gene            complement(940..1503)
                     /gene="gmk"
                     /locus_tag="VI541_03245"
     CDS             complement(940..1503)
                     /gene="gmk"
                     /locus_tag="VI541_03245"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLF69955.1"
                     /translation="MARQRLFIISGPSGAGKGTIVQGLLLRRPDIHLSVSCTTRPARP
                     MEKEGVHYHFISMADFMTRKDRGEFIEWASVHGNLYGTPRDKVEAALAGESDVILEID
                     VQGAAQVKSKWKDACLIFIEAPSMQELEQRLRRRKTEEDDDLSRRMSDAYDELRKKNL
                     FDGVIVNVDADQAVEEVLSLMDRLKEM"
     gene            complement(1500..1817)
                     /gene="mihF"
                     /locus_tag="VI541_03250"
     CDS             complement(1500..1817)
                     /gene="mihF"
                     /locus_tag="VI541_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HLF69956.1"
                     /translation="MPLPPKLSDEQRRENLAKATEARKVRAAIKEDLRTGRRTFTDLL
                     GMVEDGVVAKMKVSTLLEAVPGMGKIRAQKMMERLEISGSRHMRGLGTKQRELLAKEF
                     DPR"
     gene            complement(1878..2453)
                     /gene="pyrE"
                     /locus_tag="VI541_03255"
     CDS             complement(1878..2453)
                     /gene="pyrE"
                     /locus_tag="VI541_03255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HLF69957.1"
                     /translation="MTSDETRELLERWGALLTGHFVLSSGRHSDTYIEKFRALEHPKV
                     AWELGSELARLFSDTAIDVVLAPAVGAVILGFTTAAALGTRSIFTERVEGAMTLRRGF
                     EILPGERVLVVEDVVTTGKSLMEVVRLVPGDQLAGIGCLADRSGGIELPLPLRALTNI
                     EAVSWLPDECPLCAQGVPLTDRGSRRLGAPR"
     gene            complement(2450..3133)
                     /gene="pyrF"
                     /locus_tag="VI541_03260"
     CDS             complement(2450..3133)
                     /gene="pyrF"
                     /locus_tag="VI541_03260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HLF69958.1"
                     /translation="MTELIVALDHHDLGDAQALAKSIGDRASAFKVGHTLFTAHGPEA
                     ILEIAQHGRVFCDLKLHDIPNQVTGSAAELTRLGVWMFTVHASGGAEMVAAAVRAAKA
                     GTPPALVAAVTVLTSLSADALEGVGQGEKVQEQVLRLATLAVDAGATALVCSPQEVAL
                     LRERFPTGPKLVVPGVRPPGSETGDQARVATPREAAQAGADFIVVGRPITGATEPLAV
                     VEAVLEELR"
     gene            complement(3130..>3648)
                     /locus_tag="VI541_03265"
     CDS             complement(3130..>3648)
                     /locus_tag="VI541_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HLF69959.1"
                     /translation="DRSNMFAHDPKATASVIGAVSRSASVPVFAKLSPNVTSIVEIAS
                     AALDGGARGLSLINTVVGMAIDVDTARPKLAGVYGGLSGPAIKPIAIRAVFEVHRAFP
                     HIPLIGMGGIMDARDAIEFILAGATAVAIGTANFIDPRATLEIRNGIERFMASRGHRS
                     LAAIRGQVKTEG"
BASE COUNT          652 a         1181 c         1098 g          717 t
ORIGIN      
        1 tcgaacgcca gctcggtacc catcttgccg gaagacctgt tgccgagata cctgaccgca
       61 tcgatcgctt cacgagttcc tccggacgtg acgagaactc gaagcccggt caggtcctcc
      121 ttacgtgaga cttcgtcggc gatcgccgcc acgatgtcag cgacatccgc cagtctcccc
      181 accccaacat cgggcccagc gagcgcacca gacgcgggcc caacgaagcg cacaccgcgt
      241 gctctcagca cgccgacgtt ctcgcgggtg gctgggtctt cccacatctc ggtgtgcatc
      301 gcgggggcca tgatgatggg gcacgacgcg gagagaagga gggccgagat gaggtcctca
      361 gagatgcctt gagcgtattt ggccatcgtc ttggcggtgg ccggcgcgat gacgacgagg
      421 tccgatgtcc tgccgaggtc ggtgtgtggt atctgtgatg gcgcctcggg cgggaagagt
      481 tccgtccgga cagggttgcc agtgagtgtc gagaatgtga tctcgccgac gaagttgagt
      541 gccgacggac tcatcaccac acgaacatcc gcgcccgcga gcgtgagtgt ccgagcgagt
      601 tctacgactt tgtaagcggc gatccccccg caaaccccga gggcgatctg cttgccgctg
      661 acagcggcga ggtccatcta tctcctactt gacccgctgc tcaacctcgg gcgggcggtc
      721 gtagccgaac ttgccttggg ctatctcttc gagggcaacc gagagcgcct tggcgttctc
      781 tccgaggcga gagatctgtg ggggaacgaa gtcgcggagc ccttcaccta gttgcgagta
      841 gtaggcgttg atctgtcggg ccctcttggc agccagaacc accagcgtgt agaggctatc
      901 gaccttatcg gtaagatctt caatcttggg gtagatcact cacatctcct tgagtcggtc
      961 catcaaactt aacacctctt cgaccgcctg atcagcgtcg acgttcacaa tcactccatc
     1021 gaagaggttt ttcttgcgaa gttcgtcata cgcatcggac atccgacggg agaggtcgtc
     1081 gtcttcctcc gtcttgcggc ggcggaggcg ctgctccagc tcctgcatcg aaggggcctc
     1141 gatgaagatg agacaggcgt ccttccactt ggatttcacc tgagctgcgc cctgcacgtc
     1201 gatctcgagg atcacgtcac tttcaccggc gagagccgcc tcgaccttgt ctcgcggggt
     1261 gccatagagg ttgccgtgga cactcgccca ttcgatgaac tctccgcggt ccttcctcgt
     1321 catgaagtcc gccatcgaga tgaagtggta gtggaccccc tccttctcca tgggacgagc
     1381 cgggcgcgtt gtgcatgaga cggagagatg gatgtccgga cgccgaagca gaagcccctg
     1441 gacgatggtg ccctttccgg cccccgaggg gcctgagatg atgaagagtc gttggcgggc
     1501 tatcggggat caaactcctt agccagcagt tcccgctgtt tggtgccgag tccgcgcata
     1561 tggcgcgacc ccgagatctc cagcctctcc atcatcttct gagcccgtat cttgcccatc
     1621 ccgggtaccg cctccaaaag ggttgagacc ttcatcttgg caacaacccc gtcctcgacc
     1681 atgccgagca ggtcggtgaa ggtgcgtctt ccagtgcgga gatcctcttt gatggcggcc
     1741 cgaaccttgc gcgcttccgt tgccttggcc aggttctcgc gtcgctgctc atcgctcagt
     1801 tttggtggaa gtggcacgtt ggctccttgc gcagcaccgg aggccggatt atagaggccg
     1861 gtcgcgcaaa gcggcaatca tctgggggcc cccagcctgc ggcttccgcg gtcggtcagc
     1921 ggcactccct gagcgcagag ggggcactcg tcaggaagcc aggagaccgc ctcgatgttg
     1981 gtcagagccc gcaagggtag cggcaactcg atgccaccgg agcggtcggc caggcacccg
     2041 atgccagcaa gttgatctcc tggaaccagc ctgaccacct ccatgagtga cttgccggtt
     2101 gtgaccacat cctctaccac caggactctc tcgccgggca ggatctcgaa acctcggcgc
     2161 agtgtcatcg cgccctcgac gcgttcggtg aagatcgatc gcgttcccag tgcggccgct
     2221 gtcgtgaaac caaggatgac cgcgccgaca gccggtgcga gcacgacatc gatggcggta
     2281 tccgagaaga gacgagcgag ttcagagccc aactcccatg caactttagg atgttccagc
     2341 gccctgaact tttcgatgta ggtgtctgag tgtctgccgg aggacagcac gaagtgtcca
     2401 gttagcaggg ccccccaacg ttcgagcagc tcccgcgtct cgtcggacgt catcggagtt
     2461 cctcgagtac cgcttcgacc acagcgagag gttccgtggc cccggtgatc ggtcgtccga
     2521 cgactatgaa gtctgcccct gcctgcgccg cttcgcgcgg ggttgcgacc ctcgcttggt
     2581 cgcccgtctc cgaccctgga ggcctgaccc ccggtactac gagcttcggt ccggtcggaa
     2641 atcgctctct cagcagcgcg acctcttggg gcgagcagac gagcgcagtt gcgccggcat
     2701 ctaccgcgag ggttgccagc ctcaggacct gctcctgcac cttctcgccc tgacccacac
     2761 cttcgagcgc gtcggcagac agactggtta gaacggtcac tgcagcaacg agcgcaggcg
     2821 gggtcccggc cttggctgcc ctcaccgcgg cggcaaccat ctcggctccc ccggacgcgt
     2881 ggaccgtgaa catccagaca cccagccggg tcagctctgc agcagaaccc gtcacctggt
     2941 tcggtatgtc atgcagcttg aggtcacaga agacccttcc gtgctgagcg atctcgagga
     3001 tcgcctcggg cccgtgggcg gtgaagagtg tgtgtccaac cttgaacgcc gatgcgcgat
     3061 cgcctatcga cttcgcaagg gcctgtgcat cccccaggtc gtggtggtca agtgcaacta
     3121 tcagctcggt catccctctg tcttcacctg tcctctgatc gctgcgaggc tccggtgtcc
     3181 ccgcgacgcc atgaatctct cgatcccatt tcggatctcc aacgtcgcac gcgggtcgat
     3241 gaagttagcg gtgcctatcg ccacagccgt tgcccccgcc aggatgaact cgatggcgtc
     3301 acgcgcatcc atgatccctc ccatcccgat caacgggatg tgggggaatg cgcgatggac
     3361 ctcgaacacc gcccttatgg cgatcggttt gatcgcagga cccgagaggc ccccgtacac
     3421 acccgcaagc ttcggcctgg cagtgtcgac atctatggcc ataccaacaa cggtgttgat
     3481 gagactgagc cccctggcgc caccgtcaag agccgcactc gcgatctcca cgatgctggt
     3541 cacgttcggg gagagcttcg cgaacacagg gaccgaggct gagcggctca ccgctccgat
     3601 caccgaggcc gtcgcctttg gatcatgggc gaacatgttg cttcggtc
//
LOCUS       DATDOZ010000166         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3299338, whole genome shotgun sequence.
ACCESSION   DATDOZ010000166 DATDOZ010000000
VERSION     DATDOZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299338"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VI541_03270"
     CDS             complement(<1..588)
                     /locus_tag="VI541_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLF69960.1"
                     /translation="MPWEGSKIPATFDLAPLLVFWEVTRACGLSCRHCRAAAIEQALP
                     GELSTEEGMDLIRGLSEFGPRQPVLILTGGDALMRKDVLALASLATSQGMPLALAPSV
                     TPLLTEEVVHRAKALGVRAMSVSLDGATPTTHDGIRGVPGHHERTLAAIEMLVRSGLT
                     VQINTAVMRRNVEELAAIAELVGTLGAQIWEVFFLI"
     gene            complement(551..1054)
                     /locus_tag="VI541_03275"
     CDS             complement(551..1054)
                     /locus_tag="VI541_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69961.1"
                     /translation="MFVYYFCHVAGPFEKARAIFEDHPQRWLPAYLEKAFDRGDALRV
                     TVGIGDGFRLSKDLLVSIGEIRKDEGKVSLPLDVKASGEARLFPHLWADLELARLGEG
                     LAQLTLRGSYKPPLGWVGQMLDRALLHRIAEAAVKDFVEQIAGRLSDQTDLRGNAMGG
                     LQDSGNV"
     gene            complement(1244..1525)
                     /locus_tag="VI541_03280"
     CDS             complement(1244..1525)
                     /locus_tag="VI541_03280"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLF69962.1"
                     /translation="MTEVRLARRAVKDLDSLTNRVAHSVITAIERLAADPSDASLDVK
                     ALVGRRPWRRLRVGSYRVLFHFSDGQKVLLVGRVIDRKELEQAVKSLSD"
     gene            complement(1874..1947)
                     /locus_tag="VI541_03285"
     tRNA            complement(1874..1947)
                     /locus_tag="VI541_03285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1911..1913),aa:Glu,seq:ctc)
     gene            complement(2004..2077)
                     /locus_tag="VI541_03290"
     tRNA            complement(2004..2077)
                     /locus_tag="VI541_03290"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2041..2043),aa:Gln,seq:ctg)
     gene            complement(2126..>2398)
                     /locus_tag="VI541_03295"
     CDS             complement(2126..>2398)
                     /locus_tag="VI541_03295"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLF69963.1"
                     /translation="EKVLGQPQVGELFDRAMEILSPLEEWNRDGIEAALREVQVAMDL
                     KPKLAFIPFYVAICGSSVGAPIFDTMAILGRDESLVRLASARQLIS"
BASE COUNT          493 a          729 c          703 g          473 t
ORIGIN      
        1 gatcagaaag aagacctccc agatctgcgc tcccagagtg ccgaccagct ccgctatcgc
       61 ggctagctcc tcgacgttgc gcctcatgac cgcggtattg atctgaaccg tcagacccga
      121 ccgaacgagc atctcgatag cggccagagt tcgttcatga tgtccgggga ccccccggat
      181 cccgtcgtga gtggttgggg tggcgccgtc gagactcacc gacatggccc tcacgccgag
      241 cgccttggcg cggtgaacca cctcttcggt gagcagtggc gtgacgctgg gggccagagc
      301 cagtggcatc ccttgactcg tggccagact cgccaacgca agcacatcct tgcgcatcaa
      361 ggcgtcgccg ccggtgagga tgaggacggg ttggcggggg ccgaactcgg agagacctcg
      421 gatgaggtcc atgccctcct cggtggagag ttcacccggg agcgcttgct cgatggctgc
      481 ggcgcggcag tgcctgcagc tgagtccgca tgcacgagtt acctcccaga atacgaggag
      541 gggggcgagg tcaaacgttg ccggaatctt ggagccctcc catggcatta cctcgtaggt
      601 cggtctggtc tgataggcgc ccggcgatct gttcgacgaa gtccttgacg gcggcctcgg
      661 cgatccggtg tagaagtgcg cggtcgagca tctgaccaac ccaacccagt ggtggcttgt
      721 agctaccccg aagggtcagc tgagctagtc cttcgcccaa gcgggcaagc tcgagatctg
      781 cccacagatg cggaaatagc ctggcttctc cgctggcttt gacatcgagc gggagcgaga
      841 ccttgccttc gtcctttcgg atctcgccga tcgagaccag cagatctttc gagagtcgaa
      901 aaccatcacc gataccgaca gtgacgcgca gggcatctcc ccggtcgaag gccttctcga
      961 ggtacgcggg gagccaacgc tgtggatgat cctcgaatat cgccctcgcc ttctcgaacg
     1021 ggccagcgac gtgacagaag tagtagacga acattctctc tctccgagat cgtcattcgg
     1081 gtcgcgacgc gcgcggcgga taggtccgtc gggacagcat cttccttcga ggtgtcggtt
     1141 agacagttcc gaacggcccc tcaagggcgg gccgattgac ctgggggcct cgcccgactt
     1201 tggtgatggt ggtacgggtt tttcgagtca gcggggaagc ccctcagtcc gaaaggctct
     1261 tgaccgcttg ctccagttct ttgcgatcga tgacacgacc caccaggagc accttttggc
     1321 catcagagaa gtggaacaaa acacgatagc tccccacacg aagtcgcctc caagggcgac
     1381 gacccactaa agctttgacg tcaagactcg catctgatgg atcggcggcg agcctctcaa
     1441 tggcggtgat gacgctatgg gcaaccctgt tggtcaggga atcgagatcc ttgaccgctc
     1501 gacgcgcaag gcgtacctcc gtcacgaaac gctacaagcc ccgacgccgc ttgtacgttt
     1561 tcaggctgat ggtttttccc tgacgcaagt cagcgtcagc gtcacgtagg gatgccatga
     1621 tctcaggagc gtgagaaagc acataatcct ctgcttccgg tgagttgatg ggcaagatga
     1681 gcgcacttgg tcttccatgt cgcgttataa cgactggagc gcttccctcc tcgactgccc
     1741 gcaggatttc ggaaacacgg tttttcaagt ctctgatacc tatcgccatt gtggctacaa
     1801 ttgtagccac ttccgaagaa ccttacaacc cataggcgga agttcgaatg agagcgataa
     1861 gggtcttctg acgtaccccc gatgggtttc gaacccacga tctccgcctt gagagggcgg
     1921 tgtcctaggc cgctagacga cgggggcctg aatcgagccg aatggatcgg cccgcagata
     1981 ctaacctgct catccggtat cggctgccgg gctaggactt gaacctagaa cttcctgaac
     2041 cagaatcagg cgatctgcca aattgatcta cccggcaatg acccccaaag tttatcagtg
     2101 agcacatcga tggcttcccc accggctagc tgatgagctg tcgggcagag gcgaggcgaa
     2161 ccaacgactc gtcgcggccg aggatcgcca tcgtgtcgaa gatgggggcc cccacgctcg
     2221 aaccacatat cgccacatag aacgggatga aggccaactt cggcttgagg tccatcgcca
     2281 cctgtacctc gcgaagtgcg gcctcgatcc catctcggtt ccactcttca agaggggaaa
     2341 gaatctccat cgcacgatcg aacaactccc ctacctgcgg ctggcccaac actttttc
//
LOCUS       DATDOZ010000167         3486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3822554, whole genome shotgun sequence.
ACCESSION   DATDOZ010000167 DATDOZ010000000
VERSION     DATDOZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3822554"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..806
                     /gene="sufC"
                     /locus_tag="VI541_03300"
     CDS             66..806
                     /gene="sufC"
                     /locus_tag="VI541_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HLF69964.1"
                     /translation="MSEPILHVEDLHVSVEGKAILKGLDLKIAPGEIHAIMGKPGYQI
                     TRGRIVFKGQDLAGLAPEARARLGVFLAFQYPTEVPGVSVVNFLRTAYNSVNPEATLG
                     PLEFRKFLQTKLDLLEVSDELINRYVNAGFSGGERKRAEVLQMAVLDPQLAILDETDT
                     GLDIDALREVSEGVKRVHNEERGMLVITHYQRLLNYIEPGFVHVLLGGRFVRSGGKDV
                     ALDLEAKGYEQFRAELGISEDEELTDEA"
     gene            857..2092
                     /locus_tag="VI541_03305"
     CDS             857..2092
                     /locus_tag="VI541_03305"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HLF69965.1"
                     /translation="MDANEVRKDFPALHQMVNGHPLVYLDNGATSQRPQAVIDELKRF
                     YEQDNANVHRSVHQLGERATVLYEAARDKVADLIGAPSSRGIIFTRGATEGLNLISYA
                     WARHHLTPGDEVIVTEMEHHSNLIPWQMACTDTGATLRAVPVIDGERLDLDEYRRLLS
                     SKTKIVAVGHVSNVLGTINPVAEMAALAHEVGALVVVDGAQAVPHMPVDVTQLGCDFY
                     VATGHKMCGPTGIGFVWGREEILENMEPFHGGGEMILDVWIDHATWNEIPYKFEAGTP
                     PFAQAIGLGAAIDYLNGLGMKAIHDHEVELTKYAIAKLSDLDGMVIYGPHEADRKGGL
                     VSFTLGDIHAHDVGTICDSFGVAVRAGHHCAKPLMRKLGVPATTRASFYVYNTTEDVD
                     SLHHALQETQKMFAGGGVF"
     gene            2089..2535
                     /locus_tag="VI541_03310"
     CDS             2089..2535
                     /locus_tag="VI541_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HLF69966.1"
                     /translation="MSSLDELYKEVILDHYRAPRNRGHIDEAEVHLHRDNPLCGDEID
                     LYVDIQDGKVVRVAFDGKGCSISQASVSMMTEKLKGLSVAEAEGLANMFKQMMQGENP
                     QNPDSLGDLIALQGVQKYPVRIKCALLGWNTFLDGLRVYNEKHTGA"
     gene            complement(2532..2843)
                     /locus_tag="VI541_03315"
     CDS             complement(2532..2843)
                     /locus_tag="VI541_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69967.1"
                     /translation="MIAQVARDYVGRVEFLTVPGEDSVQAMARFLEEFEWPDSMTHAV
                     DKDLRLREHFQVRFRGAWIFLNDDGTILFQSVTHIPETQVRANLDKLLSDAPKQEIPR
                     G"
     gene            complement(2868..3173)
                     /locus_tag="VI541_03320"
     CDS             complement(2868..3173)
                     /locus_tag="VI541_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69968.1"
                     /translation="MYVRRRVALREEVALRMATDAGGRSVRRAILAAICSLVILGGCD
                     KPAPSEGGVSFESPKAGPRAHVGSAILDFKAPKLGGGQVVGNEFANKDVAIWFWAPW"
     gene            complement(3174..>3486)
                     /locus_tag="VI541_03325"
     CDS             complement(3174..>3486)
                     /locus_tag="VI541_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HLF69969.1"
                     /translation="VEDVAAPYQRGFMAAMRRPSSRASHVEVMSQVLELLEGHDDVVI
                     EARRAIDDYKQRHEGLMQVVGRLGQLIHESGAPKLDGQTYLDPHVSEIALRSLIENPL
                     T"
BASE COUNT          720 a         1057 c         1037 g          672 t
ORIGIN      
        1 ctacgaggtc aagatcgagg acgatgccat ctggttgcgt ccctcagaca ataacggagg
       61 tcccaatgag tgaaccgatc ctgcacgtcg aagatctgca tgtaagtgtc gaaggaaagg
      121 ccatcctcaa agggctggac ctcaagatcg cgccgggcga gatccacgcg atcatgggca
      181 agcccggcta tcagattacg cgcggccgca tagtcttcaa gggtcaggac ctcgcgggcc
      241 tagccccaga agcccgtgct cggctgggcg tcttcctcgc cttccagtac ccgacggagg
      301 tgcccggggt atcagtggtc aacttcctgc ggaccgcgta caactccgtc aacccggagg
      361 ccacgctcgg ccccctcgag ttccgcaagt ttcttcagac caagctggat ctgctcgaag
      421 tctcagacga gctgatcaac cgatacgtca acgctgggtt ctctggtgga gaacgcaaga
      481 gggccgaggt tctgcagatg gccgtgctcg acccccagtt ggcgatcctg gatgagacgg
      541 ataccggtct cgacatcgat gcactccgtg aggtctccga gggcgtgaag cgcgttcaca
      601 acgaggagcg cgggatgctg gtcatcactc actaccagcg actcttgaac tacatagagc
      661 cggggttcgt tcatgttctg ctcggcggta ggttcgtgcg aagtggtggc aaggatgtcg
      721 ccctcgacct ggaagccaag gggtatgagc agttcagggc cgaactcggc atatccgagg
      781 acgaagagct caccgacgag gcgtgagccg cgacccgatg gatcggcagt gaagcgccgg
      841 cgataggatg gtgcagatgg atgcgaacga ggtacgcaaa gacttccctg ctcttcacca
      901 gatggtgaat gggcaccctc tcgtctatct cgacaacggc gccacctcgc aacgccctca
      961 ggctgtcatc gatgaactta agaggttcta cgaacaggac aacgccaacg tgcatcgctc
     1021 ggttcatcaa ctcggtgaac gtgcgacggt tctctacgaa gcggcccgcg acaaggtggc
     1081 agatctcatc ggggccccca gctctcgagg gatcatcttc actcgcggtg cgaccgaggg
     1141 cctcaacctg atctcgtacg cgtgggcacg ccaccatctc acccccggcg acgaggtcat
     1201 cgtcaccgag atggagcacc actcgaatct catcccctgg cagatggcgt gtaccgatac
     1261 gggtgcgacc ctgcgggcag taccagtgat cgatggcgaa aggctggacc tcgacgaata
     1321 ccgaaggctt ctctcctcga agaccaagat cgtggccgtc ggtcatgtct cgaacgtcct
     1381 cggaaccatc aacccggttg ccgagatggc ggcgcttgct cacgaagtcg gtgctctcgt
     1441 tgttgtcgat ggagcccagg ccgtgccgca catgcccgtc gacgtgaccc agttgggttg
     1501 cgacttctac gtcgcgacgg ggcacaagat gtgcggcccg acgggtatcg gattcgtctg
     1561 gggacgtgaa gagatactcg agaacatgga gcccttccat ggcgggggag agatgatcct
     1621 cgacgtttgg atcgaccacg ccacgtggaa cgagatccca tacaagttcg aagcggggac
     1681 ccctccgttc gctcaggcca tcgggttggg ggccgcgatc gattacctca acggattggg
     1741 gatgaaggcg atccacgatc acgaggtgga gttgaccaag tacgcgatcg caaagttgag
     1801 tgacctggat ggaatggtca tctacggccc ccacgaggcg gatcgcaagg gcgggctggt
     1861 gtcattcact ctcggcgaca tccacgctca cgatgtcggg acgatctgcg acagtttcgg
     1921 cgtggcggtg agggctggac accactgcgc caagccgctc atgcgcaaac tcggcgtgcc
     1981 cgcaacgacc cgagcctcgt tctacgtcta caacacgacc gaagacgtcg attcgcttca
     2041 tcacgcgttg caggagacgc agaagatgtt cgctggaggg ggggtctttt gagtagtctc
     2101 gacgagctgt acaaagaggt catcctcgat cactatcggg ccccgcgcaa tcgcggccac
     2161 atcgacgaag ccgaggtaca ccttcaccgc gacaatcccc tttgtggcga tgagatcgat
     2221 ctgtatgtag acatacagga cggcaaggtc gtgcgggtcg cattcgacgg gaagggatgc
     2281 tctatctcac aggcctctgt ctcgatgatg accgagaagc tgaaggggct gagtgtcgcc
     2341 gaggccgagg gtcttgcgaa catgttcaag cagatgatgc agggcgagaa cccgcagaac
     2401 ccagacagtc tcggcgatct gatcgcgctt cagggcgtgc agaagtatcc ggttcgcatc
     2461 aaatgcgccc ttctcggctg gaacacgttc ctcgacggcc tgcgcgtcta caacgaaaag
     2521 cacacggggg cctagccccg cggtatctcc tgtttcgggg catcagacag cagtttgtcg
     2581 aggttggctc gcacttgcgt ctcggggata tgggtgacgc tctggaacaa gatggttccg
     2641 tcatcattca agaagatcca ggctccccga aatctaacct ggaagtgctc ccgcagtctt
     2701 aggtctttgt cgaccgcgtg agtcatcgag tctggccact cgaactcttc gaggaaccgc
     2761 gccatcgcct ggacactgtc ctcacccggt acggttagaa actcgaccct tccaacgtaa
     2821 tctcgcgcaa cctgcgcgat catcggcgca tcctgccgac atggtcctca ccacggagcc
     2881 cagaaccaga tcgcaacgtc cttgttggcg aactcgttgc cgacgacctg gccacctccg
     2941 agtttcggcg ccttgaaatc taagatcgcg cttccgacat gagcgcgagg tccggccttg
     3001 ggactctcaa agctcacgcc tccctcgctc ggcgctggct tatcgcagcc gccaagaatg
     3061 acaagagagc agatcgcagc gagtatcgcc ctgcgcacgc ttcgccctcc tgcatcggtg
     3121 gccatccgga gcgcaacctc ttcccgcagt gcaaccctcc ttctcacgta cacctacgtt
     3181 agcggattct cgatgagcga ccgcagggct atctcgctga cgtgtgggtc gaggtaggtc
     3241 tgtccgtcga gtttgggggc ccccgactcg tggatcaact gcccgagtct gccaacgacc
     3301 tgcatcaggc cctcatgacg ttgtttgtaa tcatcgatag cacggcgtgc ttcgatcacg
     3361 acatcatcgt gaccctcgag tagttcgagt acctgagaca tcacctcgac atgagaggct
     3421 cgcgacgaag gcctgcgcat cgctgccatg aatccgcgct ggtacggcgc cgccacatct
     3481 tctacg
//
LOCUS       DATDOZ010000168         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2758602, whole genome shotgun sequence.
ACCESSION   DATDOZ010000168 DATDOZ010000000
VERSION     DATDOZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758602"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VI541_03330"
     CDS             <1..394
                     /locus_tag="VI541_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF69970.1"
                     /translation="ALEWGLVNEVVPRAQLDEAVDAMALKLHDKLPECVRYTKVQTNF
                     WRELSAAMTVPHARDWLTLHTASYETYEGVRAFREKRLPDYDEVRERFAAGDGAQLWG
                     PEIVTCAACGTEGLPRRFKHCGVCGEEL"
     gene            391..774
                     /locus_tag="VI541_03335"
     CDS             391..774
                     /locus_tag="VI541_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLF69971.1"
                     /translation="MSREVIATAGAPEAIGPYSQAIRAGELLFCSGQVGLDPVTGELV
                     EGGVAAQAERALLNLGAVLDAAGGNFNDVVKTTVFLTDIADFVEMNEIYARYFGIKPP
                     ARSTVAVRALPKGAVFEIEAIARLS"
     gene            845..1060
                     /locus_tag="VI541_03340"
     CDS             845..1060
                     /locus_tag="VI541_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HLF69972.1"
                     /translation="MRRLQINIEEEMDDALALEAARRRTSKAALIREFVGERLRRKTP
                     AIDDTIAALIGSIDIEPADIDEVVYGA"
     gene            1057..1458
                     /locus_tag="VI541_03345"
     CDS             1057..1458
                     /locus_tag="VI541_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLF69973.1"
                     /translation="MRFADSSFWIGLQVPRDSRHEDASALWRDGPGALITSDLVMGET
                     WTFLRRRATHADAVGFYDAVGKSDLVTVRHIDEDLAAEAWKWLRRRDERPYSFVDATS
                     FALMRRLRLREALAYDGDFASAGFVEVRSAH"
     gene            complement(1465..1644)
                     /locus_tag="VI541_03350"
     CDS             complement(1465..1644)
                     /locus_tag="VI541_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018743932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HLF69974.1"
                     /translation="MPQGIGAGLLIIGGVFVIQGLGLARTGSFMDRQPLWAVVGAALM
                     LAGAVLGIRNRRNRQ"
     gene            1662..2540
                     /locus_tag="VI541_03355"
     CDS             1662..2540
                     /locus_tag="VI541_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HLF69975.1"
                     /translation="MSSVLGPLVVALGGNAISPVGEVDTIENQFEHARATAARLVRLI
                     DRGWDRLVITHGNGPQVGNVVTRVEMAAEVAPWLPLDICVADTGGGMGYMIQQCLQNA
                     LAHAGLERRVATLITQVEVDAGDSAFEHPAKPIGPARRLVASPRPQRVVESEVISAMV
                     SQGIIVIAGGGGGVPVVRDGQGLKGVEAVIDKDLTAALIAHSIGATAFMILTDVDGAY
                     LDHGKIDAHRIENVSVVEMRRLWGAGAFPAGSMGPKVDAACGFIESGGTVAVIAALDD
                     LEAAIAGAAGTRISAS"
     gene            complement(2527..>2780)
                     /locus_tag="VI541_03360"
     CDS             complement(2527..>2780)
                     /locus_tag="VI541_03360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF69976.1"
                     /translation="AGLPLVGGRAGGVGDAVRDGETGFLVDGRSADETADAVIRILDD
                     SVLRETLGRNARKMAENDFNWEVIFERYEAALGRAINSR"
BASE COUNT          517 a          832 c          917 g          514 t
ORIGIN      
        1 ggcgctcgaa tgggggctgg tcaacgaggt cgtcccgaga gcgcagctcg atgaggcggt
       61 cgatgccatg gcgctcaaac tgcatgacaa gctccccgag tgcgtgcgat acaccaaggt
      121 ccagacgaac ttctggcgcg agctctctgc agcgatgacg gttccgcatg cacgcgactg
      181 gctgaccctt cacaccgcct cgtacgagac atacgagggt gtccgagcgt ttcgtgagaa
      241 gcgtctgccc gactacgatg aggtcagaga gcgcttcgcc gctggcgatg gggcgcagct
      301 gtgggggcct gagatagtca cttgcgctgc gtgcggaaca gagggactcc cgagaaggtt
      361 caagcattgt ggagtctgcg gggaggaact atgagcagag aggtcatcgc aacggcgggg
      421 gcccccgagg ccatcggccc ctattcgcag gccatcaggg caggagaact cctcttctgc
      481 tccggacagg ttggcctcga cccggttacc ggtgagcttg tcgagggcgg ggttgccgcg
      541 caggccgaga gggctcttct gaatctgggg gccgtgctcg atgccgcggg cggcaacttc
      601 aacgatgtcg tcaagaccac ggtcttcctg acagacatcg ccgacttcgt agagatgaac
      661 gagatctatg cgcgttactt cggcatcaag cccccggcga gaagcactgt ggcggtgagg
      721 gccctgccga agggcgctgt cttcgagatc gaggccatcg cgaggctctc ctagacgcgt
      781 gccagcgcca caagggtgct cctctcaaga tgtatcagtc tcagagtgat gtatgctgtt
      841 catcatgaga cgactgcaga tcaacatcga agaagagatg gatgacgccc ttgcgttgga
      901 ggccgcacgg cgccgtacct ccaaggcggc cctcatcagg gagttcgtgg gggagcgact
      961 tagacggaag accccggcta ttgacgacac catcgccgcg ctgatcggtt cgatagatat
     1021 cgagccggcc gatatcgacg aggtcgttta cggagcatga ggttcgcaga cagttcgttc
     1081 tggatcggcc ttcaggttcc gagagactcc agacacgaag atgccagcgc cctatggcgc
     1141 gatggtccgg gagcgcttat cacctcagat ctggtcatgg gcgagacctg gaccttcttg
     1201 agaaggaggg cgacccatgc tgatgccgtt ggcttctacg atgcagttgg caagagcgac
     1261 ttggtgacgg tccgccacat cgacgaagat cttgccgctg aggcctggaa atggctccga
     1321 agacgtgacg agaggcccta ctcatttgtg gatgctacga gcttcgcgct gatgcggcgg
     1381 ttgagactcc gtgaagccct ggcctacgac ggcgacttcg catccgctgg cttcgtggaa
     1441 gtgcgttctg cacactgact ctgactactg cctgtttcgt cggttccgga tcccgagaac
     1501 ggcccccgcg agcatcagtg cggcgcccac cactgcccat agcggctggc ggtccatgaa
     1561 ggaacctgta cgtgccagac cgaggccctg gatcacgaag accccaccga tgatgagcaa
     1621 acccgcgcct atcccctgtg gcatgaggca agattagccg catgtcatcg gtgttgggcc
     1681 ccctcgtcgt cgccctgggg ggcaatgcga tctcgccggt gggcgaggtc gacacgatcg
     1741 agaaccagtt cgagcatgcg cgagcgacag cagcacgtct cgtgcgtctg atcgaccggg
     1801 gttgggatcg actggtgatc acgcacggca acggccccca ggttggtaac gtcgtcacgc
     1861 gcgtcgagat ggctgcagag gtcgcgccct ggcttccgct cgacatctgt gtcgccgata
     1921 ccggcggggg catgggctac atgatccagc agtgtcttca gaacgctctt gcgcatgcgg
     1981 gtctcgaacg gcgagtggct accctgatca cacaggtcga ggtggatgcc ggggacagtg
     2041 cgttcgaaca tccagcgaaa ccgatcggtc cggcgcgtcg actggtggca tctccgcgac
     2101 cacaacgggt cgttgagtcc gaggtcatct cggcgatggt gtcgcagggc atcatcgtca
     2161 tcgctggtgg agggggcggt gtgccggtcg ttcgcgatgg gcagggcctc aagggcgtcg
     2221 aggccgtcat cgacaaagac ctaaccgcgg cattgatcgc gcactcgatc ggggcgactg
     2281 cgttcatgat cctcaccgac gtcgacggcg cctacctcga ccacggcaag atcgatgcgc
     2341 accgcatcga aaacgtgagt gtcgtcgaga tgcggagact ttggggcgcg ggcgcgttcc
     2401 ccgccgggtc gatggggccg aaggtagatg ctgcgtgcgg gttcatcgaa tcggggggaa
     2461 ccgttgccgt tatcgccgcg ctcgatgacc tcgaagcggc catcgccgga gcggcgggga
     2521 cgcgtatcag cgcgagttga tcgctcggcc caaagccgcc tcgtagcgtt cgaagatcac
     2581 ttcccagttg aagtcgttct cagccatctt gcgtgcgttg cgacccagtg tctcgcggag
     2641 cacggagtcg tcgaggatcc gtatgacggc atcggcagtc tcatcggcgc ttcggccgtc
     2701 gactaggaat cccgtctcac catctcgcac cgcatccccg acaccccctg cccggccccc
     2761 gacgagcgga agtcccgccg
//
LOCUS       DATDOZ010000169         4201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_397187, whole genome shotgun sequence.
ACCESSION   DATDOZ010000169 DATDOZ010000000
VERSION     DATDOZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397187"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..954
                     /locus_tag="VI541_03365"
     CDS             265..954
                     /locus_tag="VI541_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69977.1"
                     /translation="MEATMSADGASGTWDYIGALTGVAASVVLVVPIIDVAPPPPIFS
                     GTTILDHYLRYRSEELLSALLGGIGWLLFLWFLSSLRGWLKRAEGPEGRLANIAFAAG
                     TTLVALALVNNILPATLAFGASDADASIALAFSNLTRMTVGFAALPAGAMLLAIALIS
                     IRTTGVPRWLGAAAGIISAGTLVAIGGIFATEGPLVPAGSYSCWLFSAFLLWLAITSF
                     TVARSARTSTR"
     gene            complement(935..3787)
                     /locus_tag="VI541_03370"
     CDS             complement(935..3787)
                     /locus_tag="VI541_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF69978.1"
                     /translation="MTFILTDVEGSTRLWQSSPQEMLKAVSRLDALIPEVLEPFGGVA
                     PRDQGEGDSSVGAFGSASDATRCALELQRRMQTEAWPDDAALRVRVALHSGVGEVVNG
                     NYRGVVLSRAARLRALAHGGQTLISGTTQSLIDDHLPEGASLVDLGYVQLRDLDQPEH
                     VFQLSHPDLPGAFPPLRISQAGIGNLPHQLTSFIGRERETREVIDRLNDHRLVTITGP
                     GGSGKTRLSLKVAGDLAQGFADGVWLVDLGALKEPSQLPSTVVEALGLKHRGPGEATD
                     TLLSHMRTRHALIVFDNCEHLREATASFVLQFLRACPAVKVLATSQELLAVPGEAQIL
                     LRPLSLPRGDESLDVDAAMRSESIRLFMDRARLADPCFEIGKEDVQPLVTISRRLDGL
                     PLAIELAAAKVPILGLKQIQQRLDDRFALLAGLGGQSAQRQRTLRETVSWSHDLLPPT
                     EQVLFRRLAVFPSHFSLEAAEPVCAMGDLKQVDILELLSRLVARSVVFSVAKEGERRF
                     GMLETLRTFATERLKEADEETATMGRLIEWVESFAVRAEQGLRGPDEPWWLDLVHLEV
                     PNIHAALDHADSTGKAHSALAIASSLVRFWRVRGSAPEGRARIIRSLALYVGDDDAVR
                     AKALIAAGVLAQEQGDFAHARSLYAQALGIRRLEPPGVDLAEALLRSGAVEYATGNYD
                     EAMELYNESLALRALHSDPWTIGAALQGVGGVRYWEGDLEGARQAFEESLGFQRESGD
                     RERLSALLLSLGAVEFAMSNLDRASELFEESLALSRTLKNDGGVAQALVRLGAVGLMR
                     DRFELARPLFEESLEIQRRVHDRQGEAASLHGLSVVADNLGDHETSERLQRESLALRH
                     QIGDRAHIALSLEDLARVAGRARDFERAAVLFGAAHGLRAQIGAVIVPSDRKAHDDAL
                     REVEGELGEPFASLFEKGSGFNVDDAVAFALSVSTS"
     gene            3875..>4201
                     /locus_tag="VI541_03375"
     CDS             3875..>4201
                     /locus_tag="VI541_03375"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLF69979.1"
                     /translation="MRQLTFHEPGRVTWEDISEPASMGDVDALVAPIAVAACDLDAAL
                     MKGAFPVAGPFPLGHEFVGRVMEVGDSVDGVAVGDKVAVSFQISCGECDRCMRGITGS
                     CRQVNPG"
BASE COUNT          772 a         1351 c         1283 g          795 t
ORIGIN      
        1 gcgctcggac atcttcgtga acccggagat gtcagcgaag accatcgttc cggggatccg
       61 ccggtgagac ggcgagggcg agtcggaggg ccaggtggct accaggcgcg agacgtaggg
      121 aaggagccgg gaagtttcca ctcgagacat cattcccgct atgcgcctgg gtcacaagcc
      181 gcggcgataa ggaatcagcc aatctccaga agggtgctgg actgaccgac cggggagtct
      241 cagactacct accgaccaga cgagatggag gcaactatgt cggcggatgg agccagcggg
      301 acttgggact acatcggagc tctcacgggt gtcgcagcgt cagtggtctt ggtggtgccg
      361 atcatcgatg tggccccccc accgccgatc ttctcgggta cgacgatcct cgatcactac
      421 cttcgataca gatcagagga gctgctctca gcactgttgg gtggcatcgg ctggctactg
      481 ttcctctggt tcctcagctc tctgcgcggc tggttgaagc gcgccgaagg tccggaaggg
      541 cgactggcca acatcgcgtt cgcagcgggg acgacactgg tcgcgcttgc cctggtgaat
      601 aacatcctcc cggccaccct tgcgttcggg gcaagcgatg cggacgcgtc tatagccctc
      661 gcgttctcca accttacgag gatgacggtc ggattcgcgg cactcccagc cggggcgatg
      721 cttcttgcga tcgcgctgat ctcgatccga accacgggcg tgccccgctg gctcggcgcc
      781 gccgctggca tcatctcggc aggcactctg gtagcgatcg gagggatctt cgcaacagag
      841 ggcccccttg ttccagcggg gagctactcc tgctggctct tcagcgcgtt cctactgtgg
      901 ctcgcgatca cgagtttcac ggtggcgcgg tcggctagga cgtcgacacg ctaagagcaa
      961 acgccacagc gtcgtcgacg ttgaagcccg agcccttctc gaacagggag gcgaatggtt
     1021 ctccaagttc tccctcgacc tcgcgcaacg cgtcatcgtg tgccttccga tcggacggaa
     1081 cgatcactgc gccgatctga gcacgcaggc catgtgcggc gccgaatagc accgcagctc
     1141 tctcgaagtc tcgggccctc ccagccacac gtgcaaggtc ttcgagggag agcgcgatgt
     1201 gggcccggtc tccgatctga tgccgaagtg ccagcgattc gcgttgtaac cgctcactcg
     1261 tttcgtgatc accgagattg tcggccacta cggacagtcc atggagcgac gccgcctctc
     1321 cctgccgatc atgcaccctc cgctggatct cgaggctctc ttcgaagagg ggccgggcga
     1381 gttcgaatct gtctctcatc agcccaaccg caccgagcct gaccagagcc tgggctacgc
     1441 cgccgtcatt cttcaaggtt ctcgacaggg cgagactctc ttcgaagagt tctgaagctc
     1501 tgtcgaggtt cgacatcgca aactcaactg cgccgagact cagcagcagg gcgctgaggc
     1561 gctcgcgatc cccggattcg cgctggaagc ccaggctctc ctcgaaagcc tgcctggctc
     1621 cctcgagatc tccctcccag tagcggacac caccgacccc ctgcagagcg gcgccgatgg
     1681 tccatgggtc ggagtggagg gcgcgaagcg caaggctctc gttgtatagc tccatggctt
     1741 cgtcgtagtt gccggtcgca tactccacgg cccccgatcg caggagagcc tcggccagat
     1801 ccacaccggg gggctcgagc cgccgtatgc caagggcttg cgcatataga gaacgggcgt
     1861 gagcgaagtc accctgttcc tgggccagaa cccctgccgc tatcagcgct ttcgctctga
     1921 ccgcgtcatc gtcgccgaca tagagagcga gcgaacggat gatccgggct cggccctcgg
     1981 gggccgagcc acgcacccgc cagaaacgaa caagactgct cgcgatggcc agtgcgctgt
     2041 gtgccttccc cgtcgagtct gcatgatcga gcgccgcgtg gatgttgggg acctcgaggt
     2101 gtacgagatc tagccaccat ggctcgtccg gtccgcgaag gccctgctcg gccctaacag
     2161 cgaatgactc aacccactcg atgagtcgcc ccatggtcgc ggtttcctcg tccgcctctt
     2221 tcagcctctc cgtcgcgaaa gtccgcagcg tctctaacat cccgaagcgg cgctcaccct
     2281 ctttggcgac actgaagacc accgagcgag ccacgaggcg cgagagaagc tcgaggatat
     2341 ctacctgctt cagatccccc atagcgcata cgggttctgc ggcttcgagc gagaagtgcg
     2401 acgggaatac cgcgagtcgt cggaacagaa cctgctccgt tgggggtaga aggtcgtggc
     2461 tccagctgac cgtctctctc agggttcgtt gccgctgagc cgactggcca cccagacctg
     2521 ccagcagagc gaaacgatca tcgagtcgtt gctggatctg tttgagccca aggatcggaa
     2581 ccttcgctgc cgcaagttcg atcgccagcg gtagcccatc gagtcgtctc gagatcgtta
     2641 cgaggggctg cacatcctcc ttgcctatct cgaagcatgg gtcggcgagt ctcgctctat
     2701 ccatgaagag cctgatcgac tccgatctca tcgctgcatc cacatcgagg ctctcgtctc
     2761 cacggggcag cgagagtggg cgcaggagga tctgcgcttc accaggcacc gcgaggagtt
     2821 cttgcgaagt ggcgagcacc ttgacggcag ggcatgctct taggaactgc aggacgaacg
     2881 aggccgtagc ctcgcgaaga tgctcgcagt tgtcgaagac tatcaacgcg tggcgggtgc
     2941 gcatgtgact caacagggtg tccgttgctt cacctggccc ccgatgtttg agtccgagtg
     3001 cttcgacaac ggtggacgga agttggctcg gctccttgag cgcccccaga tcgacgagcc
     3061 acaccccatc ggcgaacccc tgagccagat caccagcgac cttcaacgaa agtcgggtct
     3121 tgccggaacc tcccggtccc gtgatcgtga ctagccggtg atcattcagt cgatctatga
     3181 cttcgcgagt ctcgcgttcg cgcccgatga agctcgtcag ctgatgcggc agattgccga
     3241 tgccagcctg ggatatgcgt aacggaggga aagctccggg tagatcggga tggctgagct
     3301 gaaagacatg ttccggctgg tccaggtcgc gtagctgcac gtagccgagg tcgacaaggc
     3361 tcgccccctc agggagatgg tcatcgatga ggctctgagt tgttcctgag atgagcgtct
     3421 gacccccgtg cgctaaggcc cgcagccgtg ccgcccgact cagaacgacc ccccggtagt
     3481 tgccattgac aacctcgccg acacctgagt gaagcgcgac gcgaacgcgc agcgcagcat
     3541 catcgggcca tgcttccgtc tgcatcctgc gctgcagctc cagggcacaa cgggtcgcgt
     3601 cggatgctga gccgaaagca ccgaccgatg agtcgccctc gccctggtct cgcggcgcta
     3661 ccccgccgaa cggctcgagc acctcaggga tgagggcgtc cagtcgcgag actgccttca
     3721 acatctcttg gggactcgac tgccagagac gcgtagaacc ctctacatcc gtcaagatga
     3781 aggtcacgac ccccgtcggc agttcccgca taggtactgt ctacactggt catccctccg
     3841 cgtaaacatg atccgaccgc taacatctct cccgatgcgt caactcacgt tccacgaacc
     3901 cggccgtgtg acctgggaag acatctctga accggcatcg atgggcgatg tggacgcgct
     3961 tgttgctccc atcgccgtgg ccgcttgcga cctcgatgct gccctcatga agggggcctt
     4021 cccggtcgca ggacctttcc ccttgggtca cgagttcgtc gggagggtca tggaggttgg
     4081 cgactccgtg gatggcgtcg ccgtcggaga caaagtggct gtctccttcc agatctcttg
     4141 cggcgagtgc gaccgctgca tgcgcggtat caccggtagt tgtcgtcagg tcaaccctgg
     4201 c
//
LOCUS       DATDOZ010000170         6463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3826206, whole genome shotgun sequence.
ACCESSION   DATDOZ010000170 DATDOZ010000000
VERSION     DATDOZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826206"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VI541_03380"
     CDS             <1..357
                     /locus_tag="VI541_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69980.1"
                     /translation="STEGYSEEVLLAVFSNIPEFTYFNNLPLLVAPKASLEAGLDVYA
                     MRRFPMHYIPALAWSIFRSGTHIRYKHASYIHDRTHYSLSSSGPAFPVQVDGEYIGDR
                     TEVVIEQVPNGLAILV"
     gene            374..1147
                     /locus_tag="VI541_03385"
     CDS             374..1147
                     /locus_tag="VI541_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69981.1"
                     /translation="MVRILVLAALLLLASWWGQTALTIAVAIVAVGAGLVMMKAGANR
                     GSVGPLVVGVLAASYPTTASLLHYSTGPGSSRLPGHLLLLSAGSLVVLTTIQVLHRQR
                     EAVVRLVSSGFFFVMYAGLPATFVIMIRAGAHGTRLLVTFVVILAAYLLGSRVAGKLL
                     RSTSLRDRPPVVTWPVGLIGTLVASLGGFAVGAARVVPVSSTSLLVIGALVGITAVLG
                     EVAATMMHNESSTSRSDSLLHIAVFSAPAFYYALALYLV"
     gene            complement(1127..1501)
                     /locus_tag="VI541_03390"
     CDS             complement(1127..1501)
                     /locus_tag="VI541_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HLF69982.1"
                     /translation="MYSEALISRFLSASHAGEVEQVSAMGTEGNPTCGDVVQISLDMR
                     GGVIEDARFKTLGCAIAIAVSDVLCELATGLTQTQARTIDMARVRAVIGEVPETREHC
                     AQAPLDAFFDALMKAGSDQVQG"
     gene            1551..2759
                     /locus_tag="VI541_03395"
     CDS             1551..2759
                     /locus_tag="VI541_03395"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HLF69983.1"
                     /translation="MLDGFALWVACGYSEPMPTTEEVREVLATINDPELHRSIVELGM
                     VKGIDISAGSVTVDLALTIPGCPLKSYFQEVLPAKIKGAFSEITEVQINLGSMTEEER
                     GRLVGGVKKDVAAPFAQADSSTRVIAIGSGKGGVGKSTTTVNLAAALAKRGHTVGLLD
                     ADVWGFSIPRMLGSAQQPTVIDQRLIIPISAFGFKMISIGNFVDEDKPVIWRGPMLHK
                     ALQQFLSDVHWEEPEYLLIDMPPGTGDIALSLSQFVPGSDLILVTTPQLAAAKVAERA
                     GHMANNVGMHVSGVIENMAYAICEHCGERTYPFGSGGGKELAHRFEVPLLGQIPLDPP
                     MRDFADHGKPSVIALPDSPSALAFEVTVDAILSNFPPRPKTRKRKSLPMIMSPAQQSS
                     GNGHGHAHTH"
     gene            2763..3323
                     /locus_tag="VI541_03400"
     CDS             2763..3323
                     /locus_tag="VI541_03400"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLF69984.1"
                     /translation="MKIYTRTGDDGTTGLLYGGRVSKDDIRTHAYGTTDEAVAAIGLA
                     RSFGPQASGLSELLIKVQRELFVVGAELATAAKNAGKLQEGVSKTTEAMIVALEQEID
                     RMTAIAPLPDYFIVPGSTQVSAALDLARSVVRRAERLVVALPGEGSLADGVVLRYLNR
                     LSDFLFTAARFEEAATGEAAPRSREP"
     gene            3908..4096
                     /locus_tag="VI541_03405"
     CDS             3908..4096
                     /locus_tag="VI541_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HLF69985.1"
                     /translation="MAYQPERQIELVRPVHCSFCGKSEDAVRHIVVASAATKTSSAIC
                     DECFGWVKEIFEIREAEG"
     gene            4279..4557
                     /locus_tag="VI541_03410"
     CDS             4279..4557
                     /locus_tag="VI541_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69986.1"
                     /translation="MDQSGGSWLDPFEDPRSRGAVDPLNTQDLFAEPVDDPWITGEAL
                     SQILMFEASMQFESTRCLRCSALSFTYGAMRFCSFGCPENMLPAVPSA"
     gene            4588..5733
                     /locus_tag="VI541_03415"
     CDS             4588..5733
                     /locus_tag="VI541_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69987.1"
                     /translation="MATTFFTPEHDELRRAVRRFAEEELAPHAEEWEEAQDYPDWVYA
                     RAGELGFLGLTYPQQYGGQDADYFATIVFAEEIGRCNSGGVALGLAVQTDMATPPINR
                     FGTEEQKQRYLAPCISGEKIACLGITEPDAGSDVMNISTTATWDGGSWVINGRKIFIT
                     NGYRANFMTLVAKTRRDGADTGHSLFLVDSDTPGFSRTRKLDKVGMRSSDTAEITFED
                     CRVDADALLGVEGEGFKHIMWELQGERLIGAIAASAGASRMLEAAITYSKQREAFGRP
                     ISKFQAMRHKLAEMQTKVTALREFIYYTAYRWNLGEYVVREISMAKLLSGQIGFKVAD
                     ECLQVFGGFGYSMEFPIQRGWRDTRLNRIGGGTDEVMREVIGKLEGL"
     gene            5734..>6463
                     /locus_tag="VI541_03420"
     CDS             5734..>6463
                     /locus_tag="VI541_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF69988.1"
                     /translation="MELSREAVFPEVFRPTEVSYGLFEEDHHALRKTIRSFVEKELSP
                     NVEAWEADEDYPREIFQRVGELGLFGMKFPEESGGSGPDYIAETVLVEELAAMGSAGV
                     AADLGAHRDLACLYVYNFGNAEQKARWLVPALKGELLGALGVTEPGAGSDVAGIQTRA
                     VRDGDSYVLSGSKIFITNGSWCDFAVIAAKTDPEAGHGGITLFVVDADTPGFTRRRMK
                     MLGWRTSHTGELTFDDCRVPVENVL"
BASE COUNT         1229 a         1942 c         1974 g         1318 t
ORIGIN      
        1 agtacagagg gctacagcga ggaggtcctt ctcgccgttt tctcgaacat ccctgagttc
       61 acgtacttca acaaccttcc cctacttgtg gctccgaagg cttccctcga ggccgggctc
      121 gatgtctacg cgatgcggcg cttcccgatg cactacatac ccgctctggc ctggtcgatc
      181 tttcgtagcg gaacccacat ccgctacaag cacgccagtt acatccacga tagaactcac
      241 tactccctca gttcgagtgg accggctttc cccgttcagg tcgatggcga atacataggg
      301 gatcgaaccg aggttgtcat cgaacaggta cccaacggtc ttgcgatcct ggtctgagat
      361 cggggagatc cccttggtcc gcatcctcgt tctcgccgcc ctgcttctgc tcgcttcgtg
      421 gtggggacag actgcgctca ccatcgccgt cgcgatcgtc gccgtcggag caggactcgt
      481 catgatgaag gcaggagcca accgggggtc tgttggccca ctggtggtcg gagttctggc
      541 tgcttcgtat ccgacgacgg cctctctgct ccactattcg accggaccgg gatcctcgcg
      601 tctgccgggt catcttctgc ttctgagcgc tggttctttg gtcgtgctga cgacgatcca
      661 ggttttgcat cgacaacgag aggccgtcgt gagactcgtc tcgtcaggct tcttcttcgt
      721 catgtacgcg ggcctcccag cgacattcgt aatcatgatc cgggcggggg cacatggaac
      781 gagactgctc gtcaccttcg tagtgatact cgctgcgtac ctgctcggtt cgcgtgtcgc
      841 tggaaaactg ttacgcagta cgagtcttcg tgatcgcccg ccggtcgtca cctggccggt
      901 tggtctgatc ggaaccctcg tcgcctcgtt gggggggttc gccgtggggg cggcaagggt
      961 ggtcccggtg tcgtcaacgt cgctgctggt gatcggggcc ctcgttggga tcaccgctgt
     1021 cctgggagaa gttgcagcga ccatgatgca caacgagtcg tcgacttccc gctcggactc
     1081 gctcctgcat atcgcagtct tctcggcccc cgccttctat tacgccctag ccctgtacct
     1141 ggtctgaccc ggccttcatc agcgcgtcga agaacgcgtc gaggggggcc tgggcgcagt
     1201 gttcgcgcgt ttcgggaacc tccccgatga cagcgcgcac tcgagccatg tcgatcgttc
     1261 tcgcctgggt ctgcgtgagc ccagttgcga gttcacagag cacgtccgag actgcgatcg
     1321 cgatcgcgca cccaagtgtc ttgaatcggg catcttcgat gacaccgcct cgcatatcga
     1381 gggatatctg cacgacatcg ccgcaggtgg ggtttccctc ggtccccatc gcgctgacct
     1441 gctcgacctc accggcgtgt gacgccgaca ggaaccgcga tatgagggcc tccgagtaca
     1501 tgcttccatc ttaggcgcac caaccctcag ctgccctacg caggtactcg gtgctagatg
     1561 gctttgccct ttgggtcgcg tgcggctact ctgaacccat gccaaccacc gaagaggtcc
     1621 gcgaggtcct cgcgaccatc aatgacccgg aacttcaccg cagcatcgtc gaactcggca
     1681 tggtgaaggg catcgacatc tcggccggtt cggtgaccgt cgatctcgcg ctgaccatac
     1741 ccggctgccc tctgaagtcc tacttccagg aggtcctccc cgccaagatc aagggagcct
     1801 tcagcgagat cacggaggtc cagatcaacc tcgggtcgat gaccgaagaa gagcggggac
     1861 ggctggtggg tggcgtgaag aaggacgttg ccgccccgtt cgctcaggcc gactcgtcga
     1921 cccgcgtcat cgccatcgga agtggcaagg gcggtgttgg caagtcgacc acgaccgtca
     1981 acctggcggc ggctctagcc aaacgaggac acacggtcgg acttctcgat gcagatgtct
     2041 ggggtttctc gatcccgcgc atgttggggt cggctcaaca gccgaccgtc atagatcagc
     2101 gactgatcat cccgatctcc gcattcggct tcaagatgat ctcgatcggc aacttcgtcg
     2161 atgaagacaa gccggtcatc tggcgaggcc ctatgctgca caaggccctt cagcagttcc
     2221 tatccgatgt gcattgggaa gagcccgagt atctgttgat cgacatgccc ccagggaccg
     2281 gtgacatcgc actgtctctg tctcagttcg ttcccgggtc ggacctgatc ttggtgacga
     2341 cccctcagtt ggcggcggct aaagtcgcag agcgggccgg tcacatggcg aacaacgtcg
     2401 gcatgcatgt ctctggcgtt atcgaaaaca tggcatacgc gatctgtgaa cactgcgggg
     2461 agaggaccta cccattcggc tcgggaggag gcaaggagct ggctcatcgt ttcgaggtcc
     2521 ccttgctcgg ccagatcccg ttggatcctc cgatgcgcga cttcgccgac cacggcaagc
     2581 catcggtgat cgctctgccc gattcgccgt ctgcgctggc tttcgaggtg acggtagatg
     2641 cgatcctctc gaacttcccg cccaggccca agacgcgcaa gcgcaagagc ctgccgatga
     2701 tcatgtcgcc cgcgcagcag agttctggca acggacacgg gcacgcgcac acgcactgac
     2761 ctttgaagat ctacacgcgc acaggcgacg atggcacgac cggtcttctc tatggtggcc
     2821 gtgtctcgaa ggacgacatc cggacccacg cgtatggcac caccgacgaa gccgtcgcgg
     2881 cgataggact agctcgctcc ttcggccccc aagcgtccgg actgtccgaa cttctcatca
     2941 aggttcagcg cgagcttttc gtcgtggggg ccgaactcgc aaccgcggcg aagaacgcag
     3001 gcaaactaca agagggtgtg tcgaagacga ctgaggcgat gatcgttgca ctagagcagg
     3061 agatcgatcg gatgactgcg atagcgccgc ttcccgacta cttcatagtg ccgggttcga
     3121 cacaggtcag cgcggccctc gatcttgcgc ggtcggttgt tcgacgcgcc gagaggttgg
     3181 tcgtggcgct acccggtgaa ggctcgctcg ccgatggcgt tgtgctgcgg tatctcaatc
     3241 gactctcgga cttcctattc accgcggcac ggttcgagga ggcagctacc ggcgaggccg
     3301 ccccgcgcag ccgcgaaccc tgacctcaca gtcactcgga cgatttgacc ccctaacaac
     3361 cacggggctg tgttgatctg caagcctggt tgcgattgac acttgaacgc gccccttcag
     3421 atgacccaag gcagggagct taagacgatt cggcttgtct ggagggcttt gccatccttc
     3481 gccttctgtt ggtgagtgtg ttctcagaga ttagaagtca acccaaacct ggggcagtcc
     3541 caggtggctt gggaagcctt tctttcgctt ggctacgcgt cagccgtgca aggaggattg
     3601 ctcccaccga ggaggtgagg gcactgaagc gactgcgaat ccatttcgta atcttacctg
     3661 caaggccctc tttccgaact agaagggact gggccgatct cgactgtaga ggcagcctcc
     3721 gacgaagttc aaaatatcgg ctgtagtgag aatggcgtgt gttgggacgt tgtaagcggg
     3781 tcggtaactg acctccagat ctacaaggac tgccgggcca cggggtacag caagtccgta
     3841 tgcgtaaagg ccgctaccac cggggctgag tgaatctgtc aaccgcgcgc tttaggagga
     3901 gatacccgtg gcgtaccagc ctgagagaca aatagaactc gtccggccag tgcattgttc
     3961 cttctgcggc aagagcgagg atgcggtccg gcacatcgtc gttgcctcag ctgcaactaa
     4021 gactagttcg gctatttgcg acgagtgctt cggatgggtg aaggaaatct tcgagataag
     4081 agaagccgag ggttgatcta acacgaaagt cgcgaagccc cagcgccata ttgacggaga
     4141 cagcgcctgg cgcatcatcc catcaactcc tgttcgacaa ggctgttgga atgtggatgc
     4201 ccaagctgat gttggctttc gacaactcga ctttccaaat ccgctgaact tggcgatcac
     4261 ggtgccagga tggatgacgt ggaccagtcg ggtggttcgt ggttggaccc cttcgaggac
     4321 cctcgctcca gaggggctgt tgatcccctc aacacccagg acctattcgc cgaacctgtc
     4381 gatgacccct ggatcaccgg cgaggctctc tcgcagatcc tcatgttcga agcttcgatg
     4441 cagttcgagt caacccgatg cttgagatgc tccgcgctta gcttcaccta tggcgcgatg
     4501 aggttctgct cattcggctg tccagagaac atgctcccag ccgttccatc tgcctgatcg
     4561 ctccgcatcg cgcgctagac ttcgctcgtg gcaaccacgt tcttcacccc tgagcacgat
     4621 gaactgcgtc gcgcggtcag gcgcttcgcc gaggaagagc tcgcgcccca tgccgaggag
     4681 tgggaagagg cgcaggacta cccagactgg gtgtacgcaa gggcgggcga actcggcttc
     4741 ctagggctga cctatcctca gcagtacggg ggccaggatg ccgactactt cgcgactatc
     4801 gtcttcgccg aagagatcgg gcgctgtaac tctggcggcg ttgccctggg ccttgcggtg
     4861 cagacggata tggccacgcc tccaatcaac agattcggaa ccgaggagca gaagcagcga
     4921 taccttgccc cctgtatctc gggtgagaag atcgcttgcc tcgggataac agagccagac
     4981 gcgggctctg atgtcatgaa catatcgacc accgcgacat gggatggcgg gtcctgggtg
     5041 atcaacggcc gcaagatctt catcacgaac gggtacaggg cgaacttcat gaccctcgtg
     5101 gcgaagaccc gtcgtgacgg agcggataca ggtcactctc tcttcctcgt cgactcggat
     5161 acacccggct tctcgaggac ccgcaagctc gacaaggtgg ggatgcgaag ttcggacacc
     5221 gcagagataa cgttcgagga ttgtcgcgtt gatgccgatg ccctgctcgg tgtcgagggt
     5281 gaaggtttca agcacatcat gtgggagctg cagggcgaga gactcatcgg cgcgatcgcc
     5341 gcgtcggcgg gggcgtcccg gatgctcgaa gcggcgatca cctactcgaa acagcgagag
     5401 gctttcgggc ggcctatcag caagtttcag gcgatgcggc acaagctcgc cgagatgcag
     5461 accaaggtca ctgcgctgcg tgagttcatc tactacacgg cctaccggtg gaacctgggt
     5521 gagtatgttg tgcgcgagat ctcgatggcg aagctcctct ccgggcagat cgggttcaag
     5581 gttgccgacg agtgcctcca ggttttcggc gggttcggat actcgatgga gttcccgata
     5641 caacgcggtt ggcgcgacac gcgactcaac cggatcggcg ggggaacaga tgaagtcatg
     5701 cgcgaggtaa taggcaagtt ggaggggctc tagatggaac tgtccaggga ggccgtgttc
     5761 cccgaggtct tccgtcctac cgaagtctcg tacggtctct tcgaagagga ccaccacgcc
     5821 cttcgcaaga cgatccggag tttcgtcgag aaagagctgt ccccgaatgt cgaagcctgg
     5881 gaggccgacg aagattatcc ccgcgagatc ttccaacggg tcggcgagtt gggtctgttc
     5941 gggatgaagt tccccgagga gtccgggggt tcgggccccg actacatcgc cgagaccgtt
     6001 ttggtcgagg agctggctgc tatgggatcg gccggggtcg ccgcggacct gggggcccat
     6061 cgagacctcg cttgtctcta cgtctacaac ttcggaaacg ctgagcagaa ggctcgctgg
     6121 ctggtacccg cgctgaaagg tgagctgctg ggggccctcg gcgtcaccga accaggagcg
     6181 ggctccgatg tcgctggcat ccagacccga gcggtgcgcg acggcgactc ctatgtcttg
     6241 agcggcagca agatcttcat cacgaacgga agttggtgcg acttcgctgt gatcgcggcc
     6301 aagactgatc ccgaagcagg ccatggaggc atcaccctgt tcgtggtcga tgccgacacc
     6361 ccaggattca cgcggaggcg catgaagatg ctcggttggc gcacatcaca caccggagag
     6421 ctgaccttcg acgattgccg tgttcccgtc gagaacgtgt tgg
//
LOCUS       DATDOZ010000171         3209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1724264, whole genome shotgun sequence.
ACCESSION   DATDOZ010000171 DATDOZ010000000
VERSION     DATDOZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724264"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VI541_03425"
     CDS             complement(<1..500)
                     /locus_tag="VI541_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HLF69989.1"
                     /translation="MIATSNLPSWFIDGATLGLGLVMGSFANVVAYRVPRRISLGVSR
                     SRCPTCLHTLAWWENIPLLSYLLLAGKCRNCKRQISIRYPIVEIITATGWFVVVRSIG
                     LHPELPAFLWFVTTLVILSVIDLEHRRIPWRVVFPSFIAGVLLLFLAALRAEDLGTWG
                     RALAGAG"
     gene            complement(525..1625)
                     /locus_tag="VI541_03430"
     CDS             complement(525..1625)
                     /locus_tag="VI541_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HLF69990.1"
                     /translation="MSISEVTKPVPATSEGLRQLLEILIARKGSDLHLTAGAPPVVRV
                     HGILERLDEFDRLEPPVLQAMLYSILTQRQSDRLEEDLELDLSYSLPDIARFRVNIYF
                     QKDAMGAAFRLIPVEILSLEQLGLPEQLSIFTKMQRGLVLVTGPTGQGKTTTLASLID
                     IINVERACHIVTIEDPIEFLHQHRRSIINQREVGSDTHSFAGALRHVLRQDPDVILVG
                     EMRDLETMAIALSAAETGHLVLATLHTQDAAQTVDRIIDVFPPNQQEQIRVQLSGSLQ
                     GIVTQQLLTTREGDGRVPATEVLFATPAVRNLIREAKTYQIYSVLQSGAKHGMQTMDQ
                     SLANLVRRGKIAYETALDRCHDIQGIKQLLGG"
     gene            complement(1635..>3209)
                     /locus_tag="VI541_03435"
     CDS             complement(1635..>3209)
                     /locus_tag="VI541_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLF69991.1"
                     /translation="FLGRVLIDKGFVTESNLFALLAKQIGLDFVDLTEIQIDAAAASL
                     IPESLARRYKALPIGFVDGKLVVAMSDPANVVARDDIRTLTGQDVKAVVATGSDILAA
                     LSRLQRLDRTVESLANSAVEAIEEEFPVGEVRDLAEEAPIVKLVSSIIGSAVADRASD
                     IHIEPQERDLRIRYRIDGVLHEVLRPSKTIQAGVISRLKIMAEMDIAERRVPQDGRIG
                     LSVQGRSIDLRVASLPTVYGEKLVLRILDKSSVLLRLEDLGFSQFSYETFRAACAKPY
                     GMILVTGPTGSGKSTTLYATLNILNQPDRNIITVEDPVEYRLAGVNQIQVNNKAGLTF
                     ASALRSILRADPDIILIGEIRDRETALIAVESALTGHLVLSTLHTNDAPSAMSRLIEM
                     GLEPFLVASAVDAVLAQRLARRLCEKCKEPYKPTREALIENNFPLEEEEEIPTLYRAV
                     GCAKCGKTGYRGRMAIHEVMLMTEEIERLAVERRTSDDIKRLALEQGMRSLRTDGMEK
                     VKMGMTSLEEIQRVIV"
BASE COUNT          642 a          938 c          943 g          686 t
ORIGIN      
        1 ccggcccccg caagcgcgcg accccatgtt ccgaggtctt cagctcgcag agccgccagg
       61 aagaggagca ggactccagc gatgaatgac gggaatacga ccctccaggg gatccttcga
      121 tgctcaagat cgatcaccga gaggatcacc agtgtcgtaa cgaaccagag aaatgcgggt
      181 agctcgggat gaagtccgat ggatctgacc acgacgaacc atcccgttgc cgtgatgatc
      241 tccacgatcg gatatcggat ggatatttgc cgtttgcagt tgcgacactt acctgcgagg
      301 agaagatatg agagcagggg gatgttctcc caccaggcga gggtatgaag acacgtggga
      361 cagcgagacc gcgagacccc aagcgagatc cgacgcggga cgcgatacgc cacgacattc
      421 gcaaaactcc ccatcacgag cccgaggccg agtgtggccc catcgataaa ccatgagggg
      481 aggtttgacg tcgcgatcaa cttgcgctcg tcgccgctgg cctctcagcc acctagcagc
      541 tgcttgatac cttggatgtc gtggcaccga tccaacgctg tttcgtatgc tatcttcccg
      601 cggcggacca gattcgctag agactggtcc atggtctgca tgccgtgctt tgccccagac
      661 tgcaggactg aatagatctg ataggtcttg gcctcgcgga tgaggttcct aaccgctgga
      721 gtagcgaata acacctcggt cgctggcacc ctcccatcgc cttctcgcgt tgttagcagc
      781 tgctgagtga cgatcccttg aagactccct gatagctgaa ctctgatctg ttcctgctga
      841 ttcggaggga agacatcgat gatccggtca acggtttgag cggcatcctg agtgtgcagg
      901 gtcgcgagga ccaggtgtcc ggtctcagcc gcgctaagcg ctatggccat cgtttccaga
      961 tctctcatct ctccaacgag gatcacatcg ggatcctgtc gaagcacatg tcgaagggcc
     1021 cccgcgaatg agtgagtgtc cgagccgacc tctcgctgat tgattatcga gcgtcgatgt
     1081 tggtgaagga actcgatggg gtcttcgatg gtgactatgt ggcaggcgcg ctcgacattg
     1141 atgatgtcga tgagtgacgc cagcgtagtt gtcttgcctt gaccggtggg ccccgtcacc
     1201 aggaccaatc cacgttgcat cttggtgaag atgctgagct gctccggcaa ccccaactgt
     1261 tctaggctga gtatctcgac ggggataagg cgaaaggccg cgcccatcgc atccttttgg
     1321 aagtagatgt tgacgcggaa tcgggctatg tccgggagcg aatacgacag gtcgagttcg
     1381 agatcctctt cgagccggtc actctggcgc tgcgtcaaga tcgagtagag catcgcttga
     1441 agcacggggg gctccaggcg gtcgaactcg tcaagacgct ccagtatccc gtgaaccctc
     1501 actacaggtg gagcgccagc ggttaggtga agatcgctcc ccttgcgagc gatcagtatc
     1561 tcgagaagct gcctcaagcc ctcgctcgtt gcgggaacag gcttagtaac ctcgctgatg
     1621 gacacgccct tatcctagac gatgacccgc tgaatctctt ccaatgatgt cattcccatc
     1681 ttcactttct ccatcccgtc cgtgcgtagt gacctcattc cctgctccaa agcgagcctc
     1741 ttgatgtcgt cggaggttct tcgttcgacg gcaagcctct cgatctcctc ggtcatcagc
     1801 atcacttcat ggatcgccat cctgcctcga tacccggtct tgccgcactt ggcgcagccc
     1861 accgcacggt agagcgttgg gatctcctct tcctcttcca gggggaagtt gttctctatc
     1921 agagcctctc gggtcggctt ataaggctct ttgcacttct cgcagagccg gcgggccaag
     1981 cgctgggcga ggaccgcgtc gaccgccgag gctacgagga aaggctcgag ccccatctcg
     2041 atcaggcgac tcatcgcgct cggagcgtcg ttggtgtgca gggtcgagag tacgaggtgt
     2101 ccggtgaggg ctgactccac agcgatgagg gcggtctcgc ggtctcggat ctcaccgatc
     2161 aagatgatgt ctgggtcggc acgcaggatc gatcgcagcg ctgacgcgaa cgtgagtcca
     2221 gccttgttgt tgacctggat ctggttcaca cccgccaagc ggtattcgac gggatcctca
     2281 acggtgatga tgttgcggtc cggttgattc aagatgttca aggtcgcata gagggttgtc
     2341 gacttcccgg aaccggtcgg gcccgtgacc aggatcatgc cgtaaggttt ggcacaggcc
     2401 gcgcggaagg tttcgtagct gaactgagag aatccgaggt cttcgaggcg cagaaggacg
     2461 ctcgacttgt caaggatgcg aagcacgagc ttctcgccat acactgttgg cagactcgct
     2521 acgcgcaggt cgatagatct tccttgcacg ctcagcccga tacgtccatc ctgtgggact
     2581 ctgcgctcgg cgatatccat ctcggccatg atcttgagcc ttgagatgac tcccgcctgg
     2641 atggtcttcg atggccttag cacctcgtgg agaactccgt cgatccgata gcggatccgc
     2701 agatcgcgct cctggggttc gatatggatg tcggaggccc tatcggctac cgccgacccg
     2761 atgatcgaac tcacgagttt cacgatcggg gcctcttccg ccagatccct tacttcacca
     2821 actgggaact cctcctcgat agcctcgacc gccgagttgg cgagagactc gaccgttcga
     2881 tccagcctct gaagtcgact gagagcagcc aggatgtcgg aacctgtggc tacgacggcc
     2941 ttgacgtcct ggccggtcaa ggtcctgatg tcgtcgcgcg ccacaacatt cgcagggtcg
     3001 ctcatcgcca cgactaactt gccgtcgacg aaaccgatcg gcagggcctt gtagcgtcga
     3061 gccaggctct cggggatgag acttgcagcg gccgcgtcga tctggatctc agtcaggtcg
     3121 acgaagtcca aacctatctg cttagccaac agggcgaaga ggtttgattc agttacgaaa
     3181 cccttgtcga tgagtactcg cccgaggaa
//
LOCUS       DATDOZ010000172         5510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2498103, whole genome shotgun sequence.
ACCESSION   DATDOZ010000172 DATDOZ010000000
VERSION     DATDOZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498103"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VI541_03440"
     CDS             <1..483
                     /locus_tag="VI541_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLF69992.1"
                     /translation="LPALQPGSSIMPGKVNPVIPEAVIQVAAQVIGNDAAITIGGLNS
                     IFELNTMMPLMAYNILFSIETLAAAAHLLATRCVTGIEADEARASELMGRSLVIVTAL
                     MPLIGYDTAADVSKQAYATGKNLREIVLERGLLTEAEVDAALDVRAMTEGGLTSGGGE
                     "
     gene            480..1649
                     /locus_tag="VI541_03445"
     CDS             480..1649
                     /locus_tag="VI541_03445"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HLF69993.1"
                     /translation="MKTRLIKFGSATSTMIDPAKAGREVINRAAAHFDDGEIDLAIAF
                     FSHQHQDYIEELIAPVLAHVSPGALIGCPTPGVIGEGHEIEDGPGISLWIASFGGARV
                     DAFEVGLTETLDGMAMVGFPLGLAEPSGVILLADPTTFPTQALLASLNQDYPGMPVVG
                     GLTGAPTSQGNVLIHNNTVIATGAVGVMIGSGVEMHAVVAQGCRPIGQPLAVTAAEGH
                     HLIELAGRPPVERLREAVAGLHPSTAGLADHGFQIGIVIDDARTEPAAGDFLIRDIFR
                     ANLDTGTMIISDNVRVGQIIQFQVRDADTAEEQMALQLSGLPDVSSDAGALLFSCMGR
                     GTNMFGVPDHDALAVEASLGFAPSGMFCLGEIGPVDGRNHLHSHSISLALFSSGG"
     gene            complement(1646..2224)
                     /locus_tag="VI541_03450"
     CDS             complement(1646..2224)
                     /locus_tag="VI541_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69994.1"
                     /translation="MGRFRFLVGLAAVASIAATMATAQPVSVDSALCGGLVAGPRLQG
                     SSGPIFHVGDAISSGKAFSGGCLGAESGGYTWTLTGVASEGRLTRNRNGIEQGYFTFA
                     RPSSGDSLVGEFAGAILVSLPSPCDIGSDKVQSELVYSTVGTFVARSTDGSISKTVAG
                     SYLAKITAGMPDEKGRIPCHFQADLTGPLNAD"
     gene            complement(2307..2582)
                     /locus_tag="VI541_03455"
     CDS             complement(2307..2582)
                     /locus_tag="VI541_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69995.1"
                     /translation="MGEDEDLLKRAEEVLTQIDREHGLDDEHLEFLAALRIRIFGAPR
                     KTLDDVMKAAGEMKGRKLEEIEPPRKTTSLEDVLRKEPPPKEWPGVE"
     gene            complement(2575..3120)
                     /locus_tag="VI541_03460"
     CDS             complement(2575..3120)
                     /locus_tag="VI541_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF69996.1"
                     /translation="MNRRIAIVGSFSTGKTTLAELLSRELDLLLLPETAREVVALGFK
                     LDKDATPETEALIFLKQYNNELYARDFVGDRSLIDVMAYAGWVLDNQPRVKETAIWEE
                     CVRLAENHLRGNYTDVFYLPIEFPIVLDGLRPDDPVFQKEIDERLIKLLRSHDIPYET
                     ITGSIEERLTAIKTHLERDRG"
     gene            complement(3139..>5510)
                     /locus_tag="VI541_03465"
     CDS             complement(3139..>5510)
                     /locus_tag="VI541_03465"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLF69997.1"
                     /translation="VNAPWSLAIKAVMVERLGMDAQTMYSDDGIVIRLPEADEAPTAS
                     SIFIEPDEVEQTVTAAVGGSALFSSRFRECAARALLIPRRAPGRRTPLWQQRQRSADL
                     LQVASSYGSFPIILETMRECLQDVFDVPGLTRIMRDIQVGAMKVVEVDTVKASPFAGS
                     LLFGYIGAFLYDSDLPLAERRAQVLSLDSALLAELMGSSEMKDLIEPDVLDEVESELR
                     RSHPALRLRGPDDLHDLLRVLGELSLGEIEKRGGSAKWLEELSAHNRVVPIRIASTTY
                     WCAVEDAARFRDGLGVALPVGLPVSLLTPVPDALGGLLSRWARVHGPFTASDIAARFA
                     LDERVVTHHLSELQRRGQVRRGSYSSRDEGDEWCDVDVMRAIRRRSLAKLRSEVEPVP
                     QDVLTRAVVDRHEIGSGRGTAALLTAIGLLQGYPLAAKELERIILPCRVTDYSPAMLD
                     PLLASGEVVWVGRGAAGAKDGWVSLHLIDDLENLLPAPSEEISSGARKVVSALSDAGA
                     SFFRQLADVTGMSDQELTGWLWEAAWAGWITNDSMLPLRAMTSGLAANRSSTRVVSRP
                     GAIRQRRVVMPSVGGRWSLLPRPADPGQSAQASAMQMLDRHGVLTRGAVVSEKPPGGF
                     SAVYRLLRTFEEAGRCRRGYFVEGLGAAQFALPGAVDSMRSRPEGEVPLVLAATDPAN
                     PYGAALDWPDNGSAHRPGRKVGACVVLVESRLSAFVEKGGRSVLLFTQDPGALSEVAE
                     ALAGAVRRGWVGPVSVERVDGLDVDSSALAEFLIRAGFKRTSRGLRLRG"
BASE COUNT         1096 a         1750 c         1671 g          993 t
ORIGIN      
        1 ctccccgccc tgcagccggg gagcagcatc atgcccggca aggtgaaccc tgtgataccc
       61 gaggccgtga tccaggtcgc ggctcaggtc atcggcaacg atgcggccat cacaatcggg
      121 ggcctgaaca gcatcttcga gctcaacacg atgatgcccc tgatggccta caacatcttg
      181 ttctccatcg aaacgctagc tgcggcggca catctgctcg ccacgcgatg cgtgacaggt
      241 atcgaagcgg acgaggctcg cgcatcggaa ctgatgggcc gaagcctcgt gatcgtaacc
      301 gcgctgatgc ctttgatcgg atatgacacg gcggccgacg tttcgaagca ggcttatgcg
      361 accggcaaga acctgcgaga gatcgtcctg gagcgcgggc tgctgacgga ggccgaagtg
      421 gatgccgccc tcgatgtcag ggcgatgacc gaggggggcc tgacaagcgg cgggggcgag
      481 tgaagactcg tctgatcaag ttcggcagcg ccacctcgac catgatcgat cccgccaagg
      541 cgggacgcga ggtcatcaat cgagcggcgg ctcatttcga tgacggcgag atcgacctcg
      601 cgatcgcttt cttctcgcac caacatcagg actacatcga agagctgatc gcgcccgtcc
      661 tcgcccatgt gtcgcccgga gccttgatcg gctgtccaac gccaggtgtt atcggcgagg
      721 gtcacgagat cgaggatgga ccaggcatca gcctttggat cgcatctttt ggaggggcgc
      781 gggtcgatgc tttcgaagtg gggctgacag agacactaga tggtatggcc atggtcggat
      841 tcccactcgg actagctgaa ccgagcggag tgatccttct cgccgacccg accacgttcc
      901 cgacacaggc ccttcttgcc agcttgaacc aggactatcc tgggatgcct gtggtggggg
      961 gcctcacggg ggccccgacg tctcaaggca acgttctgat ccacaacaac acggtgatcg
     1021 cgacgggagc ggtcggcgtg atgatcggta gcggagtcga gatgcacgcc gtcgtggccc
     1081 aggggtgtcg cccgatagga caaccgttgg ccgtgaccgc cgccgaggga catcacctga
     1141 tcgagttggc cggcagaccg cccgtcgaga ggctgagaga ggcggtcgca gggctgcacc
     1201 cgagtactgc cggtctggcg gatcatggtt ttcagatcgg catcgtcatc gatgatgccc
     1261 ggaccgaacc agccgctgga gatttcctca tcagggacat cttcagagcc aacctcgaca
     1321 cgggcaccat gatcatcagc gataacgtca gggtcggtca gatcatccag ttccaggtcc
     1381 gcgacgccga cacggcagag gaacagatgg cgctccaact ttcggggctt cccgatgtga
     1441 gctccgacgc gggagccctt ctcttctcat gcatggggcg cggaacgaac atgttcggcg
     1501 tgcccgatca tgacgcgttg gccgtcgagg catcactggg cttcgccccc tccgggatgt
     1561 tctgcttggg tgagatcggg ccggtcgatg gtcgcaacca ccttcacagc cacagcatca
     1621 gtttggcgtt gttcagttcg ggcggctagt cggcgttcag agggccggtc agatcggctt
     1681 ggaagtggca ggggatccgc cccttctcgt caggcatccc cgcggtgatc ttcgcaagat
     1741 aagacccagc gacagtcttg gagatagacc catctgtaga acgcgcaaca aatgtcccca
     1801 ccgtcgagta gacgagctct gactgaacct tgtcgctacc gatgtcgcat ggcgagggca
     1861 ggctcaccag gatggctccg gcaaactcgc cgaccagcga gtcgccgctg gaaggacgtg
     1921 cgaatgtgaa gtacccctgt tcgataccgt tgcgattgcg agtcaatctt ccctccgaag
     1981 cgacaccggt gagggtccat gtgtagcccc cactctcggc ccccaggcac ccgccggaaa
     2041 acgccttgcc cgatgagatc gcgtcaccca catggaagat ggggccgctc gaaccctgaa
     2101 gccgcggacc ggcaacgagc cctccgcaga gcgcgctgtc gacggagacc ggctgggcgg
     2161 tagccatggt cgcagcgata gaagctacgg cagcgagacc tacgaggaac cgaaaccttc
     2221 ccataagagc acccccctgg aacgtttggt cggagcgcga ttatcgcacc ggaaagtccg
     2281 gatgtaacta aaaacttcgg actgtcctac tcgacgcctg gccactcttt gggcgggggc
     2341 tcttttcgga ggacgtcctc cagcgatgtg gtcttccgag gaggttcgat ctcttcgagc
     2401 ttgcgaccct tcatctcacc tgccgctttc atgacatcgt ccagtgtctt tcggggggcc
     2461 ccgaagatcc tgatgcgtag ggcagcgagg aattcgagat gctcgtcgtc gagaccatgt
     2521 tcacggtcga tctgagtcag tacttcctcg gctcgtttga ggaggtcctc atcctcaccc
     2581 acgatcgcgt tccagatgag tcttgattgc ggtgagtcgt tcttcgatcg atcccgtgat
     2641 cgtttcatag gggatgtcgt gcgagcgcag gagtttgatg agacgttcgt cgatctcctt
     2701 ctgaaagaca ggatcgtccg gacggaggcc gtcgagcacg atggggaact cgatgggcag
     2761 gtagaagacg tctgtgtagt tgccacgcag atgattctcc gcaagccgca cgcactcttc
     2821 ccagatcgcc gtctctttca cgcggggttg attgtcgaga acccaacctg cgtaagccat
     2881 cacatcgatc agcgaccggt caccaacgaa gtcgcgagcg tatagctcgt tgttgtactg
     2941 cttgaggaag atgagagcct cggtctcggg tgtcgcatct ttgtcgagct tgaaaccaag
     3001 agcaacaacc tctcgcgcgg tttcgggtag gagaagcagg tctaactcgc gcgaaagcag
     3061 ttctgcgagg gtggtcttcc cagtcgagaa gctaccaact attgcgatgc gtctattcat
     3121 gcatgaatga tgacatgctc acccgcgaag cctcagccca cgcgaggtcc tcttgaaacc
     3181 agcccttatc aggaactcag cgagcgcgct cgagtcgaca tcgaggccat cgactcgctc
     3241 aacggataca ggcccgaccc agccgcgtcg caccgcgccg gcaagggcct ccgcgacttc
     3301 gctgagggcc cccgggtcct gggtgaagag cagcacgctt cggcccccct tctcgacgaa
     3361 ggccgacagt cgcgactcca caagtacgac gcacgctccg accttacgtc cgggtcggtg
     3421 agcggacccg ttatcgggcc agtcgagggc agcaccataa ggattcgcag ggtcggtagc
     3481 ggccagcacc agggggacct ctccttctgg acgcgacctc atcgaatcga cggctcccgg
     3541 cagagcgaac tgagccgcgc caagtccctc gacgaagtag ccccgcctgc acctccctgc
     3601 ctcttcgaag gtccttaaca gccggtagac ggcgctgaac ccgccggggg gcttctccga
     3661 gacgactgcc ccacgcgtga ggaccccatg gcgatcgagc atctgcatcg ccgacgcttg
     3721 cgcactctgc cctgggtccg ctggtctcgg caggagcgac cagcgacctc ccacgctggg
     3781 catgaccact cttctctgac ggatcgcacc gggtcgcgac acgaccctgg tggatgaacg
     3841 gttcgccgcg agccccgagg tcatcgcccg gagcggaagc atcgagtcgt tcgttatcca
     3901 acctgcccac gccgcctccc agagccagcc cgttagttcc tgatcgctca taccggtcac
     3961 atctgccaac tgccggaaga acgatgcgcc tgcatccgac agcgcgctca cgaccttacg
     4021 cgcaccactg gatatctcct cactcggcgc ggggagcagg ttctccaggt catcgatgag
     4081 gtgcaaactc acccaaccat ccttcgcgcc agcggcgccc cggccgaccc acaccacctc
     4141 gcccgaagcc agaagcgggt cgagcatggc gggcgagtag tcggtcaccc ggcagggcag
     4201 gatgatgcgt tccaactcct tggcggcaag tgggtaaccc tgcaggagcc cgatcgcagt
     4261 caggagggcc gcggttccac gaccgctccc gatctcatgc cgatccacca ccgcacgtgt
     4321 caagacatcc tgtggaacag gttcgacctc ggatctgagt ttcgcgaggg accgtcgacg
     4381 gatcgccctc atcacatcta cgtcgcacca ctcatcgccc tcatcgcgag atgagtacga
     4441 tccgcgcctg acctgcccgc ggcgctgaag ttccgaaagg tgatgcgtca cgacgcgttc
     4501 atccaaagca aagcgtgctg cgatatcgga ggcagtgaat ggcccatgga cccgagccca
     4561 gcgcgatagc agtccaccca gcgcgtcggg tactggtgtc aacagcgaga cgggcagccc
     4621 aacaggcaga gcgaccccaa ggccgtcgcg gaagcgagca gcgtcctcaa ccgcgcacca
     4681 gtaggtcgtg gaagcgatcc tgatggggac aacccggttg tgcgcactga gttcctccaa
     4741 ccacttcgca cttccacccc gcttctcgat ctcgcctagc gacaactccc ctagaacgcg
     4801 cagaaggtcg tgcagatcat cgggcccccg aagacgcaag gcggggtgcg acctgcgcag
     4861 ttccgactcg acttcgtcga gcacgtcggg ttcgatcagg tccttcatct cggaactgcc
     4921 catcagctcg gcaagcagcg ccgagtcgag actcaagacc tgggccctgc gttccgctag
     4981 cggcagatcg gagtcataca ggaaggcgcc gatgtaaccg aacaggagcg aaccggcgaa
     5041 cggtgatgcc ttcaccgtgt caacctccac aaccttcatc gcgcccacct ggatgtcgcg
     5101 catgatgcga gtcaagccgg ggacatcgaa cacgtcctgc aggcattccc tcatggtctc
     5161 gaggatgatc gggaaggaac cgtatgacga ggccacctgc aacaggtcgg cacttcgctg
     5221 tcgctgctgc cagagcggtg tacgacggcc aggggctcgc cgcgggatga gaagagccct
     5281 agccgcacac tctctgaatc gcgaagagaa gagggcggag ccacctaccg cagcggtgac
     5341 ggtctgctcc acttcatcgg gttcgatgaa gatcgacgat gcggttgggg cctcatcagc
     5401 ttcaggcagt ctgatgacta tcccgtcatc cgagtacatc gtctgagcgt ccatcccgag
     5461 ccgttcgacc atcacggcct tgatggcgag cgaccagggg gcgttgacgc
//
LOCUS       DATDOZ010000173         5242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3014792, whole genome shotgun sequence.
ACCESSION   DATDOZ010000173 DATDOZ010000000
VERSION     DATDOZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014792"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VI541_03470"
     CDS             complement(<1..270)
                     /locus_tag="VI541_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF69998.1"
                     /translation="MDLQGRNEAVALTDSGQIASWLLKVVAGLLLTALVLVEGIGVLV
                     AQLAVRDAADSAATAAALAVHGGATLEQAKRDAQETAESRGATLES"
     gene            complement(234..1898)
                     /gene="murJ"
                     /locus_tag="VI541_03475"
     CDS             complement(234..1898)
                     /gene="murJ"
                     /locus_tag="VI541_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HLF69999.1"
                     /translation="MAGRTGTSADLLRVNTDSAAFGRGAAVATVGTALSRATGFLRLA
                     AMAYALGITENRVADTYQLANSTPNIIFELVLGGILSSVILRVYVEVREKDGVDEGWR
                     FIQRITSLSTILLFGVAIVGFVAAPLIFRLYTIGASGADSEAQRMVGTLLLRLFIPQV
                     IFYGFSQISDAVLKAHRRFGVSMFAPVLNNLAVTATFVVFASTVPKDLRTLALIPRSG
                     VILLGLGTTLGVVALGLVPFFYMRRVGWKRERGVGLRDPRMRRLAHLSAYMLAYVATN
                     QAGLWIALVLANRVQGGVAGYQTAFVFFQLPHGLLAVSIATVIYTGLTERSVIGDQDG
                     FALRLGQGLRGIVFVTLPAIAGYIAIAPQIVRLLIEHGLAGSASTELISTVLRIWAPG
                     ILFFSIFYMLLRAFYALGDTRTPMLINLAAFAVNITVDVISFFATRDPILRIGGLALG
                     HACSYLVASTIALFIIRRRLARPFAGSLFALIPKALFASSATGVAAWAFAGGISGWLG
                     AETIATQLIQVVGAAATGLLVYAGASRILRVEEMKWIFRVAMRRSL"
     gene            complement(1862..3913)
                     /locus_tag="VI541_03480"
     CDS             complement(1862..3913)
                     /locus_tag="VI541_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70000.1"
                     /translation="MLRLGTLVTALLLTAASMTMPAAAAASPLDVTLSEFPTFLGPDT
                     TLRLTLLAHNAGPKPVGSLAVAISIHEGIQNRSQLDVTFSKRGARSRSIVGSDTISVE
                     GTVEAGETRTITVEKALNDISFFRSHPSDRTYPIRITLRSDSGDAPTIETHLIYFSLT
                     EAPPLGVALVIPLHSPRIHSDATRPSDVTDMALEKAVAPGGRIERILTALENEPQAPV
                     TISPSGTLLDMLEQMSHGHVRAVGGRRTRLGAESPQARNAASALERIQRIARSPGVRL
                     LMAPYSQTSLPTFVRAGLTDQAQRQVDASARKSITDRAGAPIAGWLLPRAGLLDDRTI
                     ADLQRLDIGAAVLSPVSVNRPAGNLTEAAPVKLKVRTGVPFDAVISDPGLETRLRDIE
                     GLGLIQARQRFLAETATIMLERPGQVRAVAAVAPLDWSVPTALLRNIIAALTASPWIK
                     GTWPDQIVEGIKPSTNRPVDTASPDTILRAGPPMPPSSYLLAIRHAQEAIDDYADLGG
                     PRDRISTLDRRLFQGIQADDWGSRVRIRSSEAFIDSVVRSVKDEFAKIHSPASQTITL
                     TSHEGVIPLSITSSLGYPVDVVLRLDSDKLRFPDGTRLSKRLENATTTFEVRTIAQAT
                     GTFPLRVSVETPFRGVHIDESRLNIRSTAFNRVGVGITAGAGIFLVSWWLAGLARRRI
                     S"
     gene            complement(3914..4681)
                     /locus_tag="VI541_03485"
     CDS             complement(3914..4681)
                     /locus_tag="VI541_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLF70001.1"
                     /translation="MIEIYFVFAGLLSGLLSGSMGVGGALLSTPLIRLGGVSPLLAIG
                     TTVPAILPSALTGAWSYWRAGLISRRHTIWIALSAGVGSVLGAIATRRVPGNGHLLMI
                     LTAGLLLFMAIRLVPDKADVRVETETSPPGRWGLLMLGLGTGFMSGLLGVGGGFVMVP
                     VLIKVFRLPIKVALGTSLAVISLTIIPNLVMQTLVHNVDWKVAGLLAAGVVPGAWAGA
                     KLSISSSDRRLRWAFAAILGVTAIIYGVAELLQISSA"
     gene            complement(4684..5118)
                     /locus_tag="VI541_03490"
     CDS             complement(4684..5118)
                     /locus_tag="VI541_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLF70002.1"
                     /translation="MRVRSAVSAGGVVVAADGRFVLTARRNFKGDLHWGLPKGTIEEG
                     ETHEQAALREATEETGLTVEMVRKLNVIDYWYVQPAQGDQPSERVHKFVHFYLMRATG
                     GDPEAHDHETEEVGFFTPEESLRQTSYSSEIEVLAAAMDILA"
BASE COUNT         1043 a         1562 c         1694 g          943 t
ORIGIN      
        1 ggattcgaga gtagctcccc tgctctcggc cgtctcctgc gcgtcacgct tcgcctgttc
       61 gagagtggct cccccatgca cggccagggc agcggccgtc gcggcactgt ctgctgcatc
      121 acgtaccgca agttgggcaa cgaggacccc gatgccctcg acgagaacca gcgcggttaa
      181 cagcaacccg gcgactactt tgagaagcca ggatgctatc tgacccgagt cggttagagc
      241 gaccgcctca ttgcgaccct gaagatccac ttcatctcct cgacgcgaag tatccgggaa
      301 gctcccgcgt acactagcag tccggtggcg gcagctccca ccacttggat caactgtgtg
      361 gctatcgttt ccgcgccgag ccacccagat atcccccctg cgaaggccca ggccgcgacc
      421 cccgtagccg aagaggcaaa gagagccttc gggatcagcg cgaagaggga acccgcgaat
      481 ggtcgggcga gccgacggcg gatgatgaaa agggcgatgg tcgaagcgac cagatacgag
      541 catgcgtggc cgagggccag ccctccgata cgcaggatgg ggtcgcgtgt cgcgaagaag
      601 gagatgacat cgaccgttat gttcacggcg aacgcagcga gattgatgag catcggcgtc
      661 cgggtgtcac cgagtgcata gaaggccctc agcagcatgt agaagatgct gaagaacagg
      721 atgccagggg cccagatccg aagcacggtc gagatcagct cggttgaagc gcttcctgct
      781 aacccatgct cgatgagaag gcgcacgatc tgtggtgcga tcgcgatata gccagcgata
      841 gctggaagag tgacgaagac gatcccacgg agcccctgcc caaggcgcag agcgaatccg
      901 tcttgatcgc cgatgaccga ccgctcggtg agcccggtgt agatgactgt tgcgatcgat
      961 acagccagca gtccgtgggg cagttggaag aagacgaagg cggtctggta cccggcaacg
     1021 ccaccctgga cacggttcgc cagcaccaga gcgatccaga gtccggcttg gttggtggct
     1081 acataggcga gcatgtacgc cgacaggtgc gcaagccttc tcatgcgagg gtcgcgcagg
     1141 cccactccgc gctcacgttt ccatccaacc cggcgcatat agaagaaggg aaccagccct
     1201 agcgcaacaa cgccgagggt cgtgccaaga ccaagcagga tcaccccgga ccggggtatc
     1261 agcgccaatg tccgaagatc cttgggaacg gttgaagcga agacgacgaa cgtcgcggtc
     1321 accgctaggt tgttgagtac cggcgcgaac atcgatacgc cgaagcggcg atgtgccttc
     1381 aacaccgcat ctgatatctg agagaaacca tagaagatga cttgaggtat gaagagtcga
     1441 agcagaagcg tgcccaccat ccgttgagct tcgctgtcgg cccccgatgc accgatggtg
     1501 tagaggcgaa agatcaacgg tgcggccacg aatccgacga tggctacgcc gaagagcagg
     1561 atcgtcgaga gtgacgtgat gcgttggatg aacctccaac cttcatcgac accatccttt
     1621 tcgcggacct cgacgtagac cctcaggatc accgaggaga ggatccctcc gagcaccagt
     1681 tcgaagatga tgttcggtgt tgagttggcc agctgatagg tgtcggccac tcggttctcg
     1741 gtgatgccca gcgcgtatgc catggcagcg agtcgtagaa atccggtcgc acgcgacagt
     1801 gcggtcccga cagtagcgac cgccgctccg cgtccgaacg cggctgagtc cgtgttcact
     1861 ctcaggagat ccgccgacgt gccagtcctg ccagccacca tgagaccagg aatatccctg
     1921 cccccgcggt gatcccgacg ccgacgcgat tgaacgccgt ggaccggatg ttcagacggc
     1981 tctcgtcgat gtgcactcct cgaaatggcg tctcgaccga gacccgcaag gggaatgtgc
     2041 cggtagcctg agcgatggtg cgaacctcga atgttgtggt cgcgttctcg agccgcttgg
     2101 agagtcgagt tccatcgggg aagcgaagtt tgtcagagtc gagtcgaagt acaacatcga
     2161 ctggatagcc gaggctggat gtgatcgaga gcggtatcac tccctcatgc gatgtcaagg
     2221 tgatggtctg gctggccgga gaatggatct tggcgaactc atctttcacg ctgcgcacga
     2281 cggagtctat gaatgcctct gacgagcgga tccggactcg gctcccccaa tcgtcggcct
     2341 ggatgccttg aaagagcctg cgatccaggg ttgagatccg atcacggggc ccgccgaggt
     2401 cggcatagtc gtcgatcgcc tcttgtgcgt gccttatggc caggagatac gacgagggtg
     2461 gcatcggggg cccagcccgc aggatcgtgt cgggactggc cgtgtcgact ggccggttgg
     2521 tcgatggctt gatgccctcg actatctggt ctggccaggt tcccttgatc cagggggatg
     2581 cggtcagagc cgcgatgatg tttcgaagca gggccgtcgg gaccgaccag tcgaggggcg
     2641 cgaccgcggc cacggcgcgc acttggcccg gtcgttccaa catgatcgtc gcggtttcgg
     2701 ccaggaaccg ctgcctagct tggatcagcc ccagcccctc gatatctcgg agcctagtct
     2761 cgaggccggg gtcgctgatg acagcgtcga acggaacccc tgttcgaacc ttgagcttca
     2821 cgggtgcggc ttccgtgagg ttgcctgcgg ggcggttgac ggagacaggg ctgagcactg
     2881 cggcaccgat gtcgagtcgc tgcaggtctg cgatggtccg atcatcgagc aggccggcac
     2941 gcggaagtag ccaaccggcg atgggggccc cagctctgtc ggtgatgctc ttgcgcgcgc
     3001 tggcgtcgac ctgtcgctgg gcctggtctg tgaggcccgc gcgaacgaag gtgggaagcg
     3061 atgtctgcga atagggagcc atcaagaggc gcaccccagg gctgcgtgcg atcctttgga
     3121 tccgttcgag agcgctcgcc gcgtttcggg cctgcgggct ctcggccccc agtcgtgtac
     3181 gacgaccccc aaccgctcgg acatggccgt gggacatctg ctcgagcatg tcgagcagtg
     3241 tgccgcttgg cgagatcgtg acgggggcct gcggttcgtt ctcgagcgcc gtcaggatcc
     3301 gctcgatcct cccaccgggg gcgacggcct tctcgagggc catgtccgtc acgtccgatg
     3361 ggcgagtcgc gtcggagtgg atgcggggag agtgaagggg tatcaccaat gcgactccca
     3421 ggggtggtgc ttcggtcaag gagaagtaga tgagatgggt ctcgatagtg ggtgcatcgc
     3481 cagaatccga acgaagcgtt atacgtatcg ggtaggtcct gtcgctcgga tgtgaacgaa
     3541 agaagctgat gtcattcaag gccttctcaa ccgtgatggt gcgcgtttca cccgcctcga
     3601 ctgtgccctc aactgagatg gtgtcgctgc cgacgatgga acgggaacga gcaccgcgtt
     3661 tgctgaatgt gacatcaagt tgcgatcggt tctggatgcc ctcatgtatc gatatcgcga
     3721 ccgcaagtga acccaccggt ttgggtccgg cattgtgtgc gaggagtgtg agcctcagag
     3781 tcgtgtccgg gccgaggaaa gttggaaact cagacagggt cacgtcgagc ggagacgctg
     3841 ccgcggcggc tggcatcgtc atcgatgcag cggtgagcag tagggcggtc accagggtcc
     3901 ctagtcgcaa catctaggca gaggatatct gcagcagttc ggccacaccg tagatgatgg
     3961 cggttacccc caggatcgct gcgaatgccc acctgagtct tcgatcggat gaggagatcg
     4021 agagcttcgc tccggcccaa gcgcccggca cgacccctgc ggcgagtagg ccggccacct
     4081 tccaatcgac gttgtgtacg agggtctgca tgaccaggtt cgggatgatc gtgagtgaga
     4141 tgacggcaag cgaggtcccc agagctacct tgatcggaag acgaaagacc ttgatcaaca
     4201 cagggaccat gacgaaccct ccgccaacac ccaggagccc cgacatgaac cccgttccca
     4261 ggccgagcat caacagtccc catcgaccgg gtgggctcgt ctcggtctcg acacggacat
     4321 ctgccttgtc gggtaccagc cggatcgcca tgaagagaag cagccccgcg gtgaggatca
     4381 tcagcagatg tccgttaccg gggacccgac gggttgcgat agctccgagg acagaaccga
     4441 cgccagcgct gagtgcgatc cagatggtgt gtctgcggct tatcaatccg gctcgccagt
     4501 aggaccaggc ccccgtgagg gccgagggca ggatcgctgg aacggtcgtg ccgatcgcaa
     4561 gaaggggaga gaccccgccg agtcgtatca gcggcgtgga gagaagggct cccccgacac
     4621 ccatgctgcc cgagaggaga cccgagagca gtcctgcgaa tacgaagtag atctcgatca
     4681 tgttcaggcg aggatatcca tcgccgccgc aaggacctcg atctctgatg agtagctggt
     4741 ctgtcggaga gactcctccg gcgtgaagaa tccgacctcc tcggtctcat ggtcatgggc
     4801 ttcggggtcg ccgccggtcg cgcgcatcaa gtagaagtgg acgaacttgt gcacgcgttc
     4861 gctgggctga tcgccctgtg cgggctgaac gtaccagtag tcgatgacat tcaacttgcg
     4921 gaccatctcg accgtcagcc ctgtctcttc ggtggcctct cggagggccg cctgctcgtg
     4981 ggtttcgccc tcctcgatgg tccccttggg taggccccag tgaaggtcgc ccttgaagtt
     5041 gcgcctcgcg gtcaggacga atcgtccgtc ggcggcgacc accacacccc cggccgagac
     5101 cgccgatcga accctcatgg tctcgtcccg ttcatcgctc gcaaggatat gaccgagaat
     5161 agatgtgtgc gacccagcgt ctacatcgac ccctcctcgt cacgtcatga tccaggccct
     5221 ggcaggccgg accgacctgc cc
//
LOCUS       DATDOZ010000174         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3015649, whole genome shotgun sequence.
ACCESSION   DATDOZ010000174 DATDOZ010000000
VERSION     DATDOZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015649"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VI541_03495"
     CDS             <1..559
                     /locus_tag="VI541_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HLF70003.1"
                     /translation="VLIINLPGAPAAAQQGLESILEILPHAVHALSGCALETHPEARV
                     LLSDPIIVAELSGEPIDPEKVVDAIRRDDCGAVATFEGRARSPSEGVVVTSLEYEAYE
                     ERATEQLRTLGSEAIARFGLGGAAVIHRVGSLAPGDLAMLVATSAPHRGDAFDSVRWL
                     VERVKADVAIWKKELTADGGRWVGL"
     gene            complement(592..1749)
                     /locus_tag="VI541_03500"
     CDS             complement(592..1749)
                     /locus_tag="VI541_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLF70004.1"
                     /translation="MWRPEDRWIARDSLEAAFAELDRLIDERDRARLTARIEGLTISQ
                     LIDEWWSRKKREIRESTCMRYESVIRVHIRPRIGHLSANELRPLDLEDYFSSVTHKTA
                     LVSRDVLRPAFRWGMANRLVVRVDGSNPLDLAKIVRSRCLGGDAEQDANATMQVNERL
                     IPGPREIEKLLVDAEERSEDRWWLYLRLAPTLGARPGELCALRGRDIDREACTFNISR
                     SANKLTLTVGAVKRASSVRSLYLGPRLFEEIGPLLDEIGPDDFLFPAMGDRCGVRRIE
                     CWTANSVYGRLMRATKRLGLPPYTPHSFRHFCATHLLDEGWPAMQVARWLGHRNDIMV
                     RLLYGAHIVEETRSQLGEAAAQLVTRPAGLRSVRPSDPHFSKVAFGGNEYQ"
     gene            complement(1957..2029)
                     /locus_tag="VI541_03505"
     tRNA            complement(1957..2029)
                     /locus_tag="VI541_03505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1994..1996),aa:Arg,seq:cct)
     gene            complement(2210..>2464)
                     /locus_tag="VI541_03510"
     CDS             complement(2210..>2464)
                     /locus_tag="VI541_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70005.1"
                     /translation="FQADYPFDWPLGLSQGQGAGRNFYAQRNDSEFINRGLDDTLWLT
                     RAHSVGHLRQTMNVLGYALMVNGLTMLEHRRRAEGLQRAA"
BASE COUNT          449 a          739 c          796 g          480 t
ORIGIN      
        1 ggtcctgatc atcaaccttc cgggggcccc ggcggcggcg cagcagggcc tcgagtcgat
       61 cctggagata cttccccacg cggtgcatgc cctgtcaggc tgtgcactcg aaacccaccc
      121 cgaagcgcgg gtgttgctct ccgatccgat catcgtcgcc gagttgagcg gcgagccgat
      181 cgatcctgaa aaggtcgtcg atgcgatccg cagagatgat tgtggcgcgg tggcgacctt
      241 cgagggccgg gcgcggtcgc cctcggaggg cgtggtggtc acgtctctgg agtacgaagc
      301 ctatgaggag cgcgccacgg aacaactgcg aaccttgggg agcgaggcta tcgctcggtt
      361 tggactcggg ggggccgcgg tgatccatcg cgtcggctcg ttggcgccgg gtgatctggc
      421 catgctcgtt gcaacctcgg cccctcatcg cggcgatgca ttcgattcag ttcggtggtt
      481 ggtcgaacgc gtcaaggcgg atgttgcgat ctggaagaaa gaactgacag ctgatggggg
      541 ccgctgggtg ggtctctaag actgcgacgc tggagtacca tatttcggca cctattgata
      601 ctcgtttccg ccaaaggcga ccttgctgaa atgtggatca gagggcctta cagaccgaag
      661 gcccgccggt cgggtcacga gctgggccgc ggcctcgccg agttgggagc gggtctcctc
      721 gactatgtgg gcgccgtaga gcaagcgcac catgatgtcg tttcggtgtc cgagccaacg
      781 tgcgacctgc atcgccggcc acccctcgtc gagcagatga gtggcgcaga agtgccggaa
      841 agagtgtggt gtgtatgggg ggaggccaag tcgtttcgtc gctcgcatga gtcgtccata
      901 aacgctgttg gccgtccagc actcgatcct gcggacgccg catcggtcgc ccatggcggg
      961 gaagagaaag tcatcggggc caatctcgtc gaggagtggg ccgatctctt cgaacagccg
     1021 ggggccgagg taaagggatc gtacgctcga ggcccgcttg acggccccga ccgtgagggt
     1081 gagtttgttc gcgctccgac tgatgttgaa agtgcacgcc tcgcgatcga tgtcgcggcc
     1141 gcgcagggcg cagagctcgc cgggccgggc accaagggtc ggagccaggc gcaggtagag
     1201 ccaccagcga tcctcgcttc gctcctctgc atcgaccagc aacttctcga tctctcgggg
     1261 gcctgggatt agtcgttcgt tgacctgcat ggtggcgttc gcgtcttgct cggcgtcacc
     1321 accaagacaa cgagaacgca cgatcttggc caggtcgagc gggtttgagc catcgaccct
     1381 cacgacgaga cggttggcca tgccccatcg aaaggcgggc cggagaacat ctcgcgatac
     1441 gagggccgtc ttgtgggtga ccgacgagaa gtaatcctcg aggtctaggg gccgtagctc
     1501 attggccgag aggtggccga tgcggggcct gatgtgaacg cggatgacgc tctcgtaacg
     1561 catgcatgtg gactctcgga tctctcgctt cttgcgcgac caccactcgt cgatgagctg
     1621 agagatcgtg agtccctcta tgcgggccgt gaggcgtgcg cggtcacgct cgtctatgag
     1681 gcggtcgagt tcagcgaagg cggcttcgag gctgtcacgt gcgatccagc gatcctcggg
     1741 acgccacatg aaccgccatc tctgacggtg acggaaaacg gtgatgccgc gatgactgat
     1801 ccgatcaagg gggggggccg aggttgccat gccgcccata ctagggaggg cctctgacac
     1861 ttcggtcacc tcgggcgttg ggtaacagat gactgcctag tgttacccaa ttgttaccca
     1921 atcggtcagt aaccgcggcg ttttacctgc ttagagtgcc cccgagagga atcgaacctc
     1981 cgacaccagg tttaggaaac ctgtgctcta tccactgagc tacgagggcg gggcatgtag
     2041 aaaacttggt agaaaactct gacctgggca tccacacgct acttctcgtt cgagtttacc
     2101 taccggaccg accccctcgc cccttcgcgc ccccgatcca tgcgcaccga ccgttcctgg
     2161 acgcgacgaa acgcacatcc ttcttatgta tgtgcggtgc ggcccccggt taggccgctc
     2221 gttgaaggcc ctcggctcgt cgccggtgct ccagcatcgt cagcccgtta accatcaggg
     2281 catatcccag cacgttcatg gtctgccgca gatgcccgac gctatgcgcc cgcgtcaacc
     2341 aaagagtgtc atctagcccc cgattgataa actcggagtc gttgcgttgc gcatagaagt
     2401 tcctcccggc accttgccct tggctcaagc ccaggggcca atcgaacgga taatccgcct
     2461 gaaa
//
LOCUS       DATDOZ010000175         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3533725, whole genome shotgun sequence.
ACCESSION   DATDOZ010000175 DATDOZ010000000
VERSION     DATDOZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533725"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VI541_03515"
     CDS             <1..242
                     /locus_tag="VI541_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70006.1"
                     /translation="TLSLQVLPAFVAALIGGLESVKGALYGSVIVGLTVGLVPTLPGI
                     GQQVGSDKVALALLAFIVMSLRGARFTATDVRSGL"
     gene            245..2326
                     /locus_tag="VI541_03520"
     CDS             245..2326
                     /locus_tag="VI541_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLF70007.1"
                     /translation="MRTVVRIVLIALVLLFPWLPFVRQSLVVNANLAGEYAIIAISLV
                     LLTGWVGQISLGQGTLVGIGAFATGLLIRNMHIPFPVNLPIVAAIASGVATALGLVAL
                     RVRGLYLAIATLIFAWMADAYLFTASWFVGRGGSSSIENRIIGRPGTVSAFDLSDKNV
                     MYLVIVAVVAATIYAVSNLRDSKTGRAFFAVRGSEMAAVSLGIDVTRYKLMAFAISGA
                     LAGIAGNLMMIDARSVSPVTFQFTESLKFLSMAVVGGIGSIGGGVAASMLFAGLNEVF
                     FRVKALNGWLDVAAVGLLLGVLLGYPGGLGALGTQTASLTARIWARIGPRFEVVSRLV
                     GAARGGLRRGRMEAKVAAMTKHGASQGSEPKPRRRYPGRRSSVTRRTLDMSFLQQASS
                     PDQPSEVAVDQGPTYVDWRSYEDARFTLPPNRSERAPILEATGIVVQFGGLTAVDHMS
                     LEVREHEIVGLIGPNGAGKTTCFNAISGLNDPTSGTIKLFGEDVTSQSVHVRAQMGVG
                     RTFQLIQLFPQLTVFENLLVATHCHNDTGVLSHLALSDRAVRVEMEMREQVGQIVDVI
                     GLHDVAHRRIAGLPFGILRQVEIARALVTRSPFLMLDEPASGLDNTETEELTRLLHFI
                     RAKLGVSILLIEHDVRMVTSVSDYMYVLDRGKPLADGLPSEIQRDPAVIAAYLGSEKP
                     GAEAEAEPALS"
BASE COUNT          406 a          721 c          732 g          495 t
ORIGIN      
        1 tcaccctctc cttgcaggta ctgcccgcat tcgtcgccgc gctgatcggg ggcctcgaga
       61 gcgtcaaggg ggccctttac ggttcggtca tagttgggct gacagtcggg ctggttccca
      121 cgcttccagg gatcggccag caggtggggt cggacaaggt cgctcttgca ctgctcgcat
      181 tcatcgtcat gtcgctgcgg ggggccaggt tcactgcaac cgatgtacgg agcggccttt
      241 gagagtgcgg acggtcgttc ggatcgtact catcgcactg gtgctcctct tcccctggct
      301 cccattcgtt cggcaatcgc tcgtagtcaa cgccaacctc gcgggcgagt acgcgatcat
      361 cgcgatatcc ctcgtactcc tcaccggttg ggtgggacag atctcgctcg gtcaagggac
      421 actcgtaggt atcggagcct tcgctaccgg actgctgatc cgcaacatgc acataccttt
      481 tccagtcaat ctcccgatcg tcgcggcgat cgcatcagga gtggctacgg cgctgggcct
      541 tgttgctcta cgagtgcggg gactgtatct ggcgatagcg acactcatct tcgcttggat
      601 ggccgacgcg tatctcttca ccgcatcatg gttcgtgggc cggggtggtt cgagttcgat
      661 cgagaaccgc atcatcggaa gaccagggac ggtctcggcg ttcgatctgt ctgacaagaa
      721 cgtcatgtac ctcgtcatcg tcgcggttgt tgcagcgacc atctacgcgg tctccaacct
      781 gcgggactct aagactggtc gcgccttctt cgcggtgcgc gggagcgaga tggccgcggt
      841 gtcgcttggt atcgatgtga ctcgttataa attgatggcg ttcgcgatct cgggggccct
      901 tgcgggtatc gccggcaacc tcatgatgat cgatgctcgc tccgtctcac cggtgacatt
      961 ccaattcact gagagcctga agttcctctc gatggctgtg gtcggtggca tcgggagtat
     1021 cggcggtggt gttgccgctt cgatgctctt cgcaggtttg aacgaggtct tcttccgcgt
     1081 aaaggctctt aacggctggc tcgatgtggc cgcagtcggg ctgctcctcg gcgttctgct
     1141 cggctacccc ggcggacttg gggctcttgg tactcagacc gcgagcctca ccgcccggat
     1201 ctgggcgcgc atcggccccc gcttcgaggt cgtttctcgt ctggtggggg cggccagagg
     1261 gggccttcga cgaggccgga tggaggcgaa ggtcgccgcc atgacgaagc acggggcgag
     1321 ccagggatct gagccgaaac cgcgccgccg atatcccggc cgtcgttcga gcgtcactcg
     1381 ccgaacgctc gatatgtcct tcctgcagca ggcgtcctca ccagatcaac catcagaagt
     1441 ggccgtcgat caaggtccga cctacgtgga ctggcgctcc tacgaagacg ctcggttcac
     1501 cctgcccccg aacaggtccg aacgagcccc catattagaa gcgaccggta tcgtcgtgca
     1561 gttcgggggc ctcacggcgg tcgatcacat gtcgctggag gtgagggagc atgagatcgt
     1621 gggcctgatc ggtccgaacg gagcaggcaa gaccacatgc ttcaacgcca tctcgggcct
     1681 gaatgacccg acctcgggaa ccatcaaact attcggcgaa gacgttacca gccagtctgt
     1741 ccacgtgcgc gctcagatgg gtgtgggccg tacatttcag ctaatccaac tcttcccgca
     1801 actaaccgtc ttcgagaatc tcctagtagc gactcactgt cacaacgaca cgggggtgct
     1861 ctcgcatctc gccttgagtg acagggccgt tcgggtggag atggagatgc gcgaacaagt
     1921 cgggcagatc gtcgatgtca tcgggttgca cgacgtggct caccgacgca tcgccgggct
     1981 cccattcggc atcctgcgtc aggtcgagat cgcgcgtgcc cttgtcacgc gctcaccctt
     2041 cctgatgctc gatgagcccg cttcgggact cgacaacacg gagaccgaag aacttacacg
     2101 tctgcttcac ttcatcaggg cgaagctcgg cgtaagcatc ctgctgatcg aacacgacgt
     2161 gcggatggtg acatcggtct ccgactacat gtatgtgctc gatcggggaa agcccctcgc
     2221 cgatgggttg ccttccgaga tccagcgcga cccggctgtg atcgcggctt acctcggaag
     2281 cgagaagccg ggggccgagg ccgaggcaga gccagcgctg agctgatgcg caggatcaag
     2341 cggcccgcgg ctct
//
LOCUS       DATDOZ010000176         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_666926, whole genome shotgun sequence.
ACCESSION   DATDOZ010000176 DATDOZ010000000
VERSION     DATDOZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666926"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1547)
                     /gene="glmM"
                     /locus_tag="VI541_03525"
     CDS             complement(213..1547)
                     /gene="glmM"
                     /locus_tag="VI541_03525"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLF70008.1"
                     /translation="MSRIFGTDGVRGVANTELTPELALDLGRAAASVLGATLARPRVV
                     LARDTRISGEMLESALAAGLASAGADVIHTGILPTPSVAHLVVATGAAAGAVVSASHN
                     PVEDNGIKFFGPDGLKLSDAVETQIEQALGSDLHRPSGLDVGRTSTLHDAKDRYIEHA
                     LAALEGRSLAGMRVVVDCANGAAYITSPEALRRAGAEVTVINAEPDGGNINVGGGSSS
                     PEVVAKEVVARGADVGLAHDGDADRVIAVDETGQVVDGDAMIAAFAVDMKREGRLRND
                     LVVATVMANLGFRKAMDAAGISIVETAVGDRYVIEAMKSHDAALGGEQSGHVILSDFT
                     TTGDGLITGLWLLRTMVLSGRRLSEVASLVQRFPQVLLNVKVKDKEVVSASERVRRFI
                     EQSEAAMAGEGRILVRPSGTEPLVRVMVEAPDHETAASVASDIAAVITQEAG"
     gene            1642..2439
                     /locus_tag="VI541_03530"
     CDS             1642..2439
                     /locus_tag="VI541_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF70009.1"
                     /translation="MPDIDINGWRIDYGDEGEGPPIVLIHGLLMDSTMFDPQVAALSD
                     RYRVITPDLRNHGASESRAEPFTQWDLMEDSIALCDRLGVETAVFGGVSQGGFQSLRA
                     ALRHPDRVSGLILIETQAGSEDPGKAPIYESMAEVVAADGWNDFILDSVAALLFGAST
                     SDDLKKHWIERWRKLEPTHALATLKAVTRREDITDRLGEITAPAIVIHGEEDVAIEME
                     RAQILADGLVNLIDFVKIPAAGHTSTVEQPAAVNEAIETFLAKAYAL"
     gene            complement(2444..>2838)
                     /gene="rpsI"
                     /locus_tag="VI541_03535"
     CDS             complement(2444..>2838)
                     /gene="rpsI"
                     /locus_tag="VI541_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLF70010.1"
                     /translation="TQELSRATGRRKEAVARVRLLPGEGKFDINDGRTLEDYFPNLAI
                     RRIILEPLRLVEREKSYDLFVRLHGGGVAGQAGALRHGISRALLEIDPDLRPVLKKGG
                     FLTRDSRIKERRKYGLKKARKAPQYSKR"
BASE COUNT          549 a          916 c          861 g          512 t
ORIGIN      
        1 accgtgaacg atcgtggccg accgggggtc acccgccgag tgtttcgaga tccccctcct
       61 cgtcaactca tgccgaagca tgagtcctgg cgctagcgat gcgatcgccg cctgccgatg
      121 aaacaagttt cagaccaaac acgactcctc tccgtctcgc agtcccttaa tgataggggc
      181 tattggcgca gatgcgaagg aacgggcgtg agctaaccag cctcttgcgt gatcaccgct
      241 gcgatgtccg atgcgacgga cgctgcggtc tcgtggtcgg gggcctcgac cattacccgg
      301 accagcggtt cggtccccga tggccggacg aggatccttc cctcaccggc catggccgcc
      361 tcactctgct cgatgaatct gcggactcgc tccgaggctg atacgacctc cttgtccttg
      421 actttcacgt tgagaagcac ctgcgggaac cgctggacca gtgaggcgac ctcggagagt
      481 cgacgaccac tcagtaccat cgtgcgcagc agccagagtc ctgtgatcag cccatcgccc
      541 gtcgtcgtga agtcgctgag gatgacgtgg cccgactgct ctccgccaag ggcagcatca
      601 tggctcttca tcgcctcgat gacgtatcgg tcccccaccg cagtctcgac gatggatatc
      661 cccgccgcat ccatggcctt gcggaacccc agattcgcca tcacggttgc gacaacgaga
      721 tcgtttcgga gccgtccttc tctcttcatg tcgacggcga aggcggcgat catggcgtcg
      781 ccgtcgacga cctggccggt ctcatccaca gcgatcacgc gatcggcgtc gccatcatga
      841 gcaagaccta cgtcggcccc cctggccacc acctccttgg cgacgacttc cggcgaggaa
      901 gacccgcccc ccacattgat gttgccccca tcgggctcag cattgatcac cgtcacctcg
      961 gcccccgctc ggcgaagagc ctcgggtgac gtgatgtacg cggcaccgtt cgcacagtcg
     1021 acgacgactc tcatccccgc cagggatctt ccctctaggg cggcgagcgc atgttcgatg
     1081 tagcggtcct tagcgtcgtg aagtgtcgag gtccgaccga catcgagacc agagggccta
     1141 tgcaggtcac tgccgagagc ctgctcgatc tgcgtctcca ccgcatcgct cagcttcaaa
     1201 ccgtcagggc cgaagaactt gatgccgttg tcctcgacag gattatgcga tgcagagacc
     1261 acggcccccg ccgccgcccc ggtggcgacg acgagatgag caaccgacgg ggtcggcagg
     1321 atccctgtat gaatgacatc ggccccagca gatgcaagcc ctgcggcaag ggccgactcg
     1381 agcatctccc ccgagatacg ggtgtcacgt gcgagcacca cccggggtcg ggccaaggtt
     1441 gcgccgagca ccgaagctgc cgctcgccca agatccaggg caagttccgg cgtcaactcg
     1501 gtgttcgcga cgccgcgaac tccatcggtt ccaaatatcc gggacatcga ccgatattaa
     1561 ccggtcgacc cagcggcggg gcaacggctt gcgcccagag acagagacag aaagactggc
     1621 acctcgaaca aaggaggcga aatgccagac atcgatatca acggatggcg catcgattat
     1681 ggggacgagg gggaggggcc cccgatcgtt ttgatacacg gactgttgat ggacagcacg
     1741 atgttcgacc cgcaggtcgc cgctcttagc gaccgatacc gcgtgataac gccggacctt
     1801 cgcaaccatg gggcgtccga gagtcgggca gagccgttca cgcagtggga tctcatggag
     1861 gacagcatcg ctctatgcga tcggcttggc gtcgaaacag ctgtgttcgg cggcgtgagc
     1921 cagggcgggt ttcaatcact gcgggcggca ctgcgacacc ctgaccgcgt atcgggactc
     1981 atcctcatcg agactcaggc ggggtccgaa gaccccggta aggcccccat ctacgaatcg
     2041 atggccgagg tcgtcgcggc cgatgggtgg aacgacttca tcctcgactc cgtcgcggct
     2101 cttcttttcg gcgcatcaac gagtgacgac ctcaagaaac attggatcga gcgctggcgc
     2161 aaactggaac cgacccacgc acttgcgact ctgaaggcgg taacacgtcg cgaggacatc
     2221 acagaccgac ttggcgagat aaccgctccg gccatcgtca tacacggtga agaggatgtg
     2281 gcgatcgaga tggaacgtgc gcagatcctc gctgatgggc tcgtcaacct gatcgacttc
     2341 gtcaagatcc ccgcggcagg ccacacgtca acggtcgaac aaccggcagc ggtgaatgaa
     2401 gcgatcgaga cgttccttgc gaaggcctac gccctctgag gatctaccgc tttgagtact
     2461 ggggggcctt gcgggccttc ttgagtccgt acttgcgtcg ctccttgatg cgcgagtcgc
     2521 gcgtcaggaa gccgcccttc tttaggaccg gccgaagatc ggggtcgatc tcgagaagcg
     2581 ctcgcgagat gccatggcgc agagcaccgg cctggcccgc gacgccgccg ccgtggagac
     2641 gaacgaacag gtcgtaggac ttctcgcgct caaccaatct caggggttcg aggatgatcc
     2701 ggcggatcgc gaggttcggg aagtagtcct cgagggtgcg gccgtcgttg atgtcgaact
     2761 tgccctctcc gggcaggagt cttacgcgtg cgaccgcttc tttgcggcgc ccggtagccc
     2821 tcgatagttc ctgcgtct
//
LOCUS       DATDOZ010000177         5857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3835034, whole genome shotgun sequence.
ACCESSION   DATDOZ010000177 DATDOZ010000000
VERSION     DATDOZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3835034"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VI541_03540"
     CDS             <1..745
                     /locus_tag="VI541_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70011.1"
                     /translation="WADNDLQVFRGGASGADILDRHDASITFDIGCARWQGTFDVGEM
                     AYITSTDGPIRAIRDYLGTNSGPLTERRHIFYESSEEIRVDLRVHPIPGASEIWNWST
                     AAVGMRYSTAFGSARVDGQGDAYLGSPAVLPQSWNWETIDRDTPADPSVGGVSIVTYY
                     DSNNPDPVAHSIYRDGGTGGCQNQPYRGAIWNQGPAALVSTDVGTPPYFGTYLYLTRT
                     LHYEAPGTTNGPMRLGQDKTPLVITGSAL"
     gene            823..2235
                     /locus_tag="VI541_03545"
     CDS             823..2235
                     /locus_tag="VI541_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLF70012.1"
                     /translation="MRTSSARIVAAALALASTLAPIAVAGPSGIVLIHGRITDAFGQP
                     LYGCIEATYALTSSRPPSVPADVEGEHGGSPAVDGSTFTDPDGYYTLAVAPGSYEITA
                     MAAYQGPTRSSCWNDGGTYVKRTRTVDTLTTLDPVDFTLPFRITADMWRDPGYYNSFT
                     PGDQIELVALLSTQIENPSASWTDQGSGSIVNMTRQLTDSPNSGPRWKFEGTFAIPSD
                     RPDGTYNSTFHIDAPGHPGIADDLVMPFIIDTVAPTAAATYPFDGATNIRTNLEPAFW
                     ATDVGVGVSCGHSDMKMYVINGGVETLVPYDYEQMGSVQDACGNGPNYNLNLGSHYRL
                     AFEISDLAGNTTAKDVHFQTGTNDVFSEASPTGTVHAPVSRIEIKADTAIDARWMSFR
                     VARQTPTGWSTINYRVPHFERATQRFWIDLDEISNDAFKTASHKPPLGPGTYRVIIQD
                     QNDMSDNPLPTYEWSFVVTT"
     gene            2281..4212
                     /locus_tag="VI541_03550"
     CDS             2281..4212
                     /locus_tag="VI541_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HLF70013.1"
                     /translation="MIRISKLNKSFGARDLFLDAEINIGARDRIALVGPNGSGKTTLF
                     EILVGSGSADSGDIQIAGDAVIGYLSQETDALRGKVLLAEVMSAGSASDLGHRMRLLE
                     AEMSAIGPGHELDTLVADYSNLEMRFASLGGYGLEHEAKTILGGLGFKTEDLARMTET
                     FSGGWLMRIALAKLLLSSPDLLLLDEPTNHLDLESVEWLERFLRSYQGAVVLISHDRD
                     LINGFASKVVEVSGCKLVSYSGDFEDFIEQRELARQQAEAASRNQARKVAQTQVFIDR
                     FRYKASKAKQVQSRIKSLDRMERLEAPKHRRRAMSLRFPQPPRAGRVVLELDDVSFGY
                     SDELVYRSLDLAIERDHKIALVGPNGAGKSTLLKLVAGALVPRSGEVRLGHRAQVAYF
                     AQHQIEALNPANRVIEEMQLALPSDATVRPRDLLGRLLFSGDDVDKPVSVLSGGERSR
                     LALGKLLVSRANLLCLDEPTNHLDMESRDVLEDSLNDYEGAMVLITHDRHLIRSVANR
                     IIEVVDGKLHVADGDYEYYISKRQEAISDPVVRVPQVSSKERRREQAEARAKVKTLVD
                     KVAHLERQVEEAAAEVQEIARRLADPAFYASAADVGEVVKSYDDKAKRLAELERLWER
                     AASDLESHRGLEKSEASGQ"
     gene            4295..4657
                     /locus_tag="VI541_03555"
     CDS             4295..4657
                     /locus_tag="VI541_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLF70014.1"
                     /translation="MASYMKKSFDTPDEVRTFAHGKLDVVSLGSHVIARTSFEPGWKW
                     SEDIKPKVGTDSCQKHHVGYATEGRLSVVTDDGDEFEIQAGDVYEVQPGHDAWVVGND
                     NFVGLEFEGKTAEEFSKK"
     gene            4816..>5857
                     /locus_tag="VI541_03560"
     CDS             4816..>5857
                     /locus_tag="VI541_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLF70015.1"
                     /translation="MTSRQPNRPRKIRLAALGIVTVVSLITPLAAGAHVAPGGYREGD
                     ESLVALNILPPGQGHHLNALEAPPAFAGMHPAHNTDQIAKYEGLIKAASTLNSSNLAD
                     WFTDASFGVKPGDVGRIYKPGYRHGLVVVRDLSKDVPHVYGTTRADTMFGAGYVSGED
                     RLFAMDALRHLGRGRLSELLGPSASNLASDRAVRLASDYDEAELEAMGQRLKVIDPVR
                     GAIAEQDLLNYSAGINQFIHDAIRDPSKLPAIYPAIQQMPQLWKPTDSIAIAAYIGGV
                     FSVGGGGQLRNAAFLKQLMDQGKTYAEARSIMEDFRFANDPEAPVSTSVAFPYNTGLG
                     PVDLSSVALPDNP"
BASE COUNT         1267 a         1723 c         1744 g         1123 t
ORIGIN      
        1 ctgggccgat aacgaccttc aggtcttccg cggtggtgcc agcggcgcgg acatcttgga
       61 tcgacacgac gcgagcatca cattcgacat cggatgcgcg cgctggcagg gcacgttcga
      121 tgtgggcgag atggcataca taacgagcac ggacgggccg atccgcgcga tccgcgacta
      181 cctcggtact aacagcggcc cgctgactga gcgaaggcac atcttctacg aatcgtccga
      241 ggagatacgt gtcgacctgc gcgttcaccc gatcccggga gcgtcggaga tatggaactg
      301 gagcactgcc gcggtcggta tgcgctactc gacagccttc ggctcggcac gggtagatgg
      361 ccaaggcgac gcttacctcg gtagtccagc cgttctgcca cagtcatgga actgggagac
      421 catcgataga gatactcccg cggatcccag tgtcggtgga gtctcgatag tgacgtacta
      481 cgacagcaac aacccggacc cagtcgcaca ctcgatctac cgggacggtg gaacaggtgg
      541 atgccagaac cagccctacc gaggcgccat atggaaccag gggccggcgg ccttggtttc
      601 gaccgacgtg gggacccctc cttactttgg gacttatctc tatctcaccc ggacacttca
      661 ctacgaggcg ccgggtacga ctaacgggcc gatgcgtttg gggcaagaca agacgccttt
      721 ggtgatcacg ggaagtgccc tttagcgcga tccactggca tccgtgccca gtttgcgtaa
      781 gagagcagga gaatcggacg aaacgccgaa gtatcccgtg acatgagaac ctcttcagcc
      841 cggatagtcg cggcggcgct ggcactggcg tcaaccctgg cccccatcgc cgtggcggga
      901 ccatccggga tcgtacttat ccatggtcgc atcacagatg cattcgggca gcccctatat
      961 ggctgtatag aagcaaccta tgccttgacg agttctcggc ccccgtcggt tcccgccgat
     1021 gttgaaggcg aacatggtgg atcacccgcc gttgatggca gcacattcac cgatcccgat
     1081 gggtactaca ccttggcggt ggcgcctggc tcttatgaaa tcacggccat ggccgcttac
     1141 caggggccga ctcgatcttc ttgttggaac gatggcggca cctatgtcaa gaggacaaga
     1201 accgttgaca cactgaccac tctcgaccca gtcgacttca ccctgccatt ccggatcacg
     1261 gctgacatgt ggcgtgatcc cggctactac aactcattca cgccgggcga tcagatcgag
     1321 ctcgtggcgc tcttgagcac tcagatcgag aacccttccg ccagttggac cgatcaaggg
     1381 tcgggatcga tcgttaacat gacgcggcaa cttaccgact ctcctaactc tggcccgcga
     1441 tggaagttcg aggggacctt cgccatccct tccgatcggc ccgatggcac ctacaactcg
     1501 accttccata tcgacgctcc cggacacccc ggcatcgccg atgacctggt gatgccattc
     1561 atcattgaca cagtggcacc gacggcggct gcgacctacc cgttcgacgg tgctacaaac
     1621 atcaggacca acctcgagcc cgccttctgg gcgacagatg ttggcgttgg tgtgagctgc
     1681 ggacactcgg atatgaagat gtacgtgata aacggcggcg tcgaaaccct cgttccttat
     1741 gactacgaac agatgggaag tgttcaagac gcctgcggga acggtcctaa ctacaacttg
     1801 aacctcggtt ctcattaccg actcgccttc gagatctccg atctagccgg gaacacgacc
     1861 gcgaaggatg ttcacttcca gacgggcacg aacgatgtct tcagcgaagc gtcgccgacc
     1921 gggacagtcc acgctcccgt gtctaggatc gagatcaagg cggacactgc gatagatgcg
     1981 cgttggatga gcttccgcgt cgccaggcag acaccgacgg gttggagcac catcaattat
     2041 cgagtccctc acttcgagag ggcgacccag cgattctgga tcgatctgga cgagatttcg
     2101 aacgatgcct tcaagacggc atcacacaag ccgcccctcg gaccggggac atatcgagtc
     2161 atcatccagg atcagaacga catgtctgac aacccgctcc ctacctatga gtggtccttc
     2221 gtcgtaacaa cttgagtctc agggagcggc tccagtgaac gccttgggct agcatggttc
     2281 atgatccgga tcagcaaact caacaagtcc tttggtgccc gcgacctctt tcttgatgct
     2341 gagataaaca tcggggcccg cgatcgcatc gcgttggtcg ggccgaatgg ctcgggtaag
     2401 acaaccctct tcgagatcct ggtcgggtcc ggcagcgccg attcgggcga tatccagatc
     2461 gcaggtgatg ccgtcatcgg ctacctcagt caagagaccg atgccctaag aggcaaggtg
     2521 ttgctcgccg aggtgatgtc ggccggatcc gcaagcgacc tgggccatcg catgagactc
     2581 ctcgaagccg agatgtccgc gatcggtccg ggccacgaac tcgacacact cgtcgctgat
     2641 tactcgaatc tggagatgcg gttcgccagt ctcggtggtt acggtctgga acatgaagcc
     2701 aagaccatcc tgggtggtct cggcttcaag accgaggacc ttgcccgcat gaccgagaca
     2761 ttctcgggcg ggtggctcat gcgtatcgcg ttggccaaac tcctgctgtc atctcctgat
     2821 ctactgttgc tcgatgagcc gaccaaccac ctcgatctgg aatcggttga gtggctcgaa
     2881 cgattccttc gttcgtatca gggcgcggtt gtgttgatct ctcacgaccg cgacctgatc
     2941 aatggtttcg cgtcgaaggt ggtcgaggtc agcggatgca agctagtttc ttattccggt
     3001 gatttcgaag acttcataga gcagagagag ctggcaaggc agcaggccga ggcagcgtcg
     3061 cgcaatcagg cccgcaaggt ggctcaaacg caggtcttca tcgatcgctt ccgatacaag
     3121 gcgagcaagg ccaagcaggt tcagagtcgc atcaagtcgc tcgatcgcat ggaacgcttg
     3181 gaagcgccga agcatcgacg acgggcgatg agtctgcgct tccctcagcc cccaagggcc
     3241 gggcgagtcg tcttggagtt ggacgacgtc tccttcggat attcagatga gttggtatac
     3301 cgatcgctcg atcttgccat cgaacgagat cacaagatcg cgttggtggg ccccaatggc
     3361 gcgggcaagt caacgctgct gaagctagtt gcaggtgcgt tggtcccaag atccggagag
     3421 gttcgattgg ggcatcgggc gcaggtcgcg tacttcgctc agcatcagat cgaggccctc
     3481 aaccccgcca accgagtgat cgaagagatg caactcgcgt tgccctcgga cgcgacggtc
     3541 cgcccccgag acctcttggg caggttgctg ttttcgggtg acgatgtcga taagccggtt
     3601 tcagttctat cgggtggaga acggtcacga ctggccttgg gcaaactgct cgtctcgcga
     3661 gcgaacctgc tttgtctcga cgaaccgacg aaccatctcg acatggaaag ccgcgatgtc
     3721 ctcgaagatt cgctcaacga ttacgaggga gccatggtgc tcatcacgca tgaccggcac
     3781 ctgatcagaa gtgtggccaa tcgcatcatc gaggtggtag atggcaagtt gcatgtggcc
     3841 gatggtgact acgagtacta catctcgaag cgtcaagagg cgatttcaga ccccgtcgtt
     3901 cgggtcccgc aggtttcgtc gaaggaacgc agacgcgaac aggctgaagc cagggcgaag
     3961 gtgaagaccc tggttgataa ggttgcgcat ctcgaaagac aggtcgaaga ggcagcggcc
     4021 gaggtccaag agatcgctcg caggttagcg gaccctgcct tctatgcctc ggcggctgac
     4081 gtcggcgagg tcgtgaagtc gtatgacgac aaggcgaaac gtctagctga gctagagaga
     4141 ctctgggaga gggcggcctc agatctggaa tcacaccgtg ggctggaaaa aagtgaagcc
     4201 tcggggcagt gaacgaagcc actgccccct cgtaacttgc gagatgacct gatagcgtgg
     4261 ccgagagatc gtcgtccgac aaaggagggc attgatggct agttacatga agaagagttt
     4321 tgacacgcca gacgaggtgc ggactttcgc ccacggaaag ctggacgtcg tgagtctagg
     4381 aagccatgtg attgccagaa cctcgttcga gcctggatgg aagtggtcag aggacatcaa
     4441 gccgaaagtc ggtaccgaca gttgccagaa gcaccacgtt ggctacgcta ccgagggtcg
     4501 tcttagtgtc gttacagacg acggtgacga gttcgagata caagccggtg acgtgtacga
     4561 agtccagccc ggccatgatg cctgggtagt aggaaacgac aatttcgttg gcctagagtt
     4621 cgaaggcaag acagccgaag agttctccaa gaagtaagcg tcatccggac caccggcccc
     4681 tacgagttgt aaggcaggtg tcccagggag agagggctgt cggctgacgc gccaagaaca
     4741 tggccttgag tcagcaggaa tctgggcaga aagggagaat ccctcctaca tgaccccagc
     4801 gaggagatcc cctaaatgac atcaaggcaa cccaaccgcc cccgaaagat tcggctcgcc
     4861 gcactgggca tcgtgaccgt cgtgtctctg ataacaccgc tcgcggcagg agcgcatgtc
     4921 gctccgggag gttatcggga aggggacgag agtctcgtgg ccctcaacat ccttcccccc
     4981 ggccagggcc accacctcaa tgccctcgag gcccccccgg cattcgctgg gatgcaccct
     5041 gctcacaaca cagaccagat cgccaagtat gagggcctga tcaaagcagc ctcgaccctg
     5101 aacagttcga acctcgcaga ttggttcacg gatgcatcct tcggcgtgaa gccgggcgat
     5161 gtcgggcgga tctacaagcc cggataccgt cacgggctcg tcgtggtgcg cgacctgtcg
     5221 aaggacgttc cgcatgtcta cggcactact cgggcggaca ccatgttcgg ggccggttac
     5281 gtgagcggcg aggaccgcct cttcgcgatg gatgcacttc gccatctggg tcgaggcaga
     5341 ctctccgaat tgctgggccc gagcgcgtcc aaccttgctt ctgaccgcgc ggtgcggctc
     5401 gcctcggact atgacgaagc ggaactcgaa gcgatgggcc agcgcctcaa ggtcatcgac
     5461 ccggttcgcg gagcgatcgc agagcaggac ctactcaact actccgcggg aatcaaccag
     5521 ttcatccatg acgcgatacg cgacccgagc aaactcccgg ccatctaccc tgccatccag
     5581 cagatgcctc agctttggaa gccgaccgac tcgatcgcga tagcggcata catcggcggg
     5641 gtattcagtg tgggtggagg tggtcagtta cgcaacgccg cattcctgaa gcagttgatg
     5701 gaccaaggca agacgtacgc ggaagctagg tccatcatgg aggacttccg gttcgctaat
     5761 gatccagagg cccccgtctc aacatcggta gccttccctt acaacaccgg cctcggcccg
     5821 gtagacctca gttccgtggc gctgccagac aatcctg
//
LOCUS       DATDOZ010000178         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1728903, whole genome shotgun sequence.
ACCESSION   DATDOZ010000178 DATDOZ010000000
VERSION     DATDOZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728903"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VI541_03565"
     CDS             <1..271
                     /locus_tag="VI541_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70016.1"
                     /translation="VARPGDDIRFDNVDILTHDVIAIETDASTSRPWCASFATGACPL
                     FWSPTVGLAGSRFVEGTENLTAGNTYHFYCSWHPFMEGTLVIAPA"
     gene            514..696
                     /locus_tag="VI541_03570"
     CDS             514..696
                     /locus_tag="VI541_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70017.1"
                     /translation="MSTNDRWTMAVNSMTPMGRSITFMVVVSLFVTMPVIVRLQMQGV
                     AISPQSHSMPKITASP"
     gene            1162..1410
                     /locus_tag="VI541_03575"
     CDS             1162..1410
                     /locus_tag="VI541_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HLF70018.1"
                     /translation="MSIRLQVVLDESEMEAIREDARRQGVTVSELVRRALKDSRRQLA
                     SGEPSRKLAAVRAAARHSFPSGSIEQMLSEIERGYEDS"
     gene            1407..1802
                     /locus_tag="VI541_03580"
     CDS             1407..1802
                     /locus_tag="VI541_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLF70019.1"
                     /translation="MILIDSNVPMYLVGADHPHKEDAARVLDDLIVKGERLVTDTEVL
                     QEILHRYSAIDRLDAIQPAFDAVLDVVDEVLAVHLAEVEEAKSVLLGRKGISARDALH
                     VAVMNANQIERIVSFDQGFDQIPGIERIS"
     gene            complement(1805..>2213)
                     /locus_tag="VI541_03585"
     CDS             complement(1805..>2213)
                     /locus_tag="VI541_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70020.1"
                     /translation="SPRARLGTVEDPVEPVALENNFDERTRTRSFHFSFAVFADSHVG
                     LLLRGTPSKHEGVTLRFAVKECRGEIAINVRLKRAIGPPRLDRFECSKRCSAPWALSG
                     IADTTPGNLLRVAFDEYTGSSLGDLQSMLRRFR"
BASE COUNT          441 a          622 c          676 g          474 t
ORIGIN      
        1 ggtggctcgc cccggagatg acatccgctt cgacaacgtc gacatcctca ctcacgatgt
       61 catcgcgatc gagacggatg catctacgtc gcggccctgg tgcgcgtcat tcgcgacggg
      121 ggcttgccca ctcttctgga gtccaaccgt cggtcttgct ggaagcaggt tcgtggaggg
      181 gaccgagaac ctcactgcgg ggaacaccta tcacttctac tgctcatggc accccttcat
      241 ggagggcacg ctggtcatcg cgcccgccta ggcgtcccga tctcgttcat tgacgtcaat
      301 gggccccgcc gctgcctgcg actcggcatg acggagcgtg gccatctgag gcagcgccga
      361 tcctctgacg gaactgctct ctctttcgtt cgacgactgc tccgatgggg aatgcgtctg
      421 ctgcgtccag cagttcatct accatgacga tgaggtaacc cgcgtcgtcg ggatcggttg
      481 ggtatccggg gccgggggtc tgtgatccat cccttgtcga ccaacgatcg ctggaccatg
      541 gccgtgaact cgatgacacc gatggggagg tcgatcacct tcatggtcgt ggtttccttg
      601 ttcgtaacca tgccagtcat cgtgcgtttg cagatgcagg gcgtggcgat ctccccgcag
      661 tcgcactcga tgccgaagat aacggcctcg ccttagggga ccaaacagaa gtcgtccctg
      721 cgccaaccct gtgtttggca agttgaaagc accttcatcg accttcaccc cttccgaact
      781 aacgtgatga catcgtagtg agggggtctg acatctaaag gtggccttac cgcggttcat
      841 gcggggtttt ctctagattt ccgtcctgga tcggatcgtc gaagcagttg aggatgtctg
      901 actggagggg ctatgcccgc tcctgaaaat tgatagccac agcgatccga gcaggacgga
      961 cccggttaag ggatttgggg acggtcattg ggccgtgccc ccgtttgttc agatgtctgg
     1021 gcaaaggaat ccggggacct gcccgcagag aagaccggtc caaaccggag caggactcga
     1081 gcgaggaccg tattcgtggt gcatcttgag ggacccgagg aagaggctag acagtagcat
     1141 ggagtatgtc atactcttcc catgagtatt cgactccaag ttgtcctcga cgagtcagag
     1201 atggaagcga ttcgagagga tgctcgccgt cagggtgtca cggtctccga gttggttcga
     1261 cgtgccctca aggactcccg ccgccaactt gcgtcgggag agcccagccg aaaactagct
     1321 gcggtccgag ccgccgctcg gcacagcttc ccctcaggca gcattgagca gatgctctcc
     1381 gagatcgagc ggggatatga agattcgtga ttctcatcga ttcgaacgtt cctatgtatc
     1441 tggttggcgc cgatcatccg cataaggagg atgcggcaag ggtgctggac gatctcatcg
     1501 tgaaggggga gaggctagtc accgacacgg aggtgctgca ggagatcctc caccgatact
     1561 cagcgatcga tcgactcgat gccatccagc ccgctttcga cgcggttctc gatgttgtcg
     1621 acgaagttct tgccgttcat ctggccgaag tcgaggaagc gaagagcgtt ctgcttggca
     1681 ggaagggcat ttcagctcgc gatgcactgc atgttgcggt catgaacgcg aaccagattg
     1741 aacgtatcgt gagctttgac cagggtttcg accagatacc gggaatcgag cgcatctcct
     1801 aagatcaacg gaaccgcctg agcatgctct ggaggtcccc gagcgatgat ccggtgtact
     1861 cgtcgaacgc gacgcggagg agattgccgg gtgttgtgtc ggcaatgccg gagagagccc
     1921 agggggctga gcatctcttc gagcactcga accgatcgag tcttgggggg ccgatcgcgc
     1981 gtttcagccg tacatttatc gcaatttccc cgcggcactc cttcaccgcg aacctgagtg
     2041 tcacaccctc gtgtttagat ggagtgcccc gcaacaggag gccgacatgg ctatctgcga
     2101 agactgcgaa agagaaatga aaactgcgag ttcgtgtacg ttcatcgaaa ttattctcga
     2161 gggcgacagg ttcgaccgga tcttctacgg ttccgaggcg ggcgcgaggg ctt
//
LOCUS       DATDOZ010000179         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_403607, whole genome shotgun sequence.
ACCESSION   DATDOZ010000179 DATDOZ010000000
VERSION     DATDOZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403607"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /gene="rplR"
                     /locus_tag="VI541_03590"
     CDS             complement(<1..313)
                     /gene="rplR"
                     /locus_tag="VI541_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLF70021.1"
                     /translation="MSHTSWTAQRAKRHRRVRKKVVGSAQRPRLAVFRSNRHFYAQLI
                     DDASGTTLASASTLEPELRSSTGRSSEVASVVGGLIAQRAIEKGIKAVVFDRGGFRFH
                     GN"
     gene            complement(310..846)
                     /gene="rplF"
                     /locus_tag="VI541_03595"
     CDS             complement(310..846)
                     /gene="rplF"
                     /locus_tag="VI541_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLF70022.1"
                     /translation="MSRIGRTPITVPSGVEVELQTGFVRVRGPKGNLERTLPSEISIA
                     REGEELVVTRPSDSGPHRSLHGLTRTLVANMVVGVTEGFERNLEIHGTGYRATPKGQD
                     LELALGYSHPVEYKAPSGITFEVPTPTRITVRGADKELVGQVAAELRALRKPDPYKQK
                     GVRYAGEYIRKKAGKTAK"
     gene            complement(859..1257)
                     /gene="rpsH"
                     /locus_tag="VI541_03600"
     CDS             complement(859..1257)
                     /gene="rpsH"
                     /locus_tag="VI541_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003854344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLF70023.1"
                     /translation="MSLTDPIADMLTRIRNATTASHDTLDMPSSKQKIDIARILKDEG
                     YIADFEVVPAKVGQSIRITLKYAEDRSKTIHGIRRISRPGLRVYAKATALPKVLGGMG
                     TAIVSTSSGLMTDKQAARKKLGGEVVAHIW"
     gene            complement(1262..1447)
                     /locus_tag="VI541_03605"
     CDS             complement(1262..1447)
                     /locus_tag="VI541_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HLF70024.1"
                     /translation="MAKKALVAKANRTPKFKVRAYTRCRRCGRPRGYLRKFGMCRLCV
                     RQLAHRGELPGITKSSW"
     gene            complement(1463..2020)
                     /gene="rplE"
                     /locus_tag="VI541_03610"
     CDS             complement(1463..2020)
                     /gene="rplE"
                     /locus_tag="VI541_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003956453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLF70025.1"
                     /translation="MATMTEIPRLKTKYLSEVRPTLATELSLNVMAVPRLLKISINMG
                     VGDVLRESKALDDAAADLALLTGQKAMITRAKKSIAAFKVREGQAIGAKVTLRGDRMW
                     EFFDRLLSVALPRIRDFRGLSPKGFDGRGNYTFGVTEQLIFPEMEYDKVAKVRGMDIT
                     LVTSATTDEQGLALLQSFGFPFKQL"
     gene            complement(2020..>2205)
                     /locus_tag="VI541_03615"
     CDS             complement(2020..>2205)
                     /locus_tag="VI541_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLF70026.1"
                     /translation="KIRPSKGSRGGQEGGIITKDLPVDLSNVTLVCNNCGVTRVGFTV
                     HEDGSKTRICKKCEAEL"
BASE COUNT          404 a          689 c          633 g          479 t
ORIGIN      
        1 cattgccgtg gaagcgaaag ccgccacggt cgaagacaac ggccttgatc cccttctcga
       61 tcgcacgttg ggcgatcaac ccgccgacga cagatgcgac ctcgctcgaa cggccagtag
      121 aagaacgtag ttcgggttcg agagtactcg cagaagcgag agtcgtcccc gatgcatcgt
      181 cgatcagctg cgcgtagaag tggcggttgc tccggaagac agccagcctc ggccgctggg
      241 cggagcccac taccttctta cgcaccctgc gatgacgctt ggccctctgt gccgtccaac
      301 tcgtatgact catttggctg tcttccctgc cttcttgcgg atgtactctc ccgcgtaccg
      361 aacacccttc tgcttgtagg ggtctggctt gcggagtgcc cttagctctg cagcgacctg
      421 gccgacgagt tccttgtccg caccgcggac agtgatccta gtgggcgtcg gcacctcaaa
      481 tgttatcccc gatggagctt tgtactcgac ggggtgagag tagccgagcg ccaactcaag
      541 gtcctgacct ttgggtgtcg cgcggtaacc ggtgccgtgt atctcgaggt tgcgctcgaa
      601 accctctgtc acaccaacaa ccatgttggc aacaagtgtt cgggtgagac cgtgcagcga
      661 acggtggggg cccgagtcgc ttgggcgcgt gacgacaagc tcctcgccct ctcgggcgat
      721 cgagatctcg gacggcagtg tccgctccag gttgcccttc ggcccccgga cgcgcacgaa
      781 ccccgtctgc agctcgacct cgacgccgct tggcaccgtg atcggggttc ttccgatacg
      841 actcacttgt actctcccct accagatgtg agcgacgact tcgccgccca gcttcttgcg
      901 tgccgcttgc ttgtcggtca tgagccccga cgaagtcgag acgatcgccg tgcccatgcc
      961 accgagaacc tttgggagcg cagtggcctt cgcgtatacg cgcaggcctg ggcgagagat
     1021 ccggcggatc ccgtggatcg tcttcgacct gtcctccgcg tacttgagtg tgatgcggat
     1081 gctctgccca accttcgcag gcacgacctc gaagtcagcg atgtagccct cgtccttgag
     1141 gatgcgagcg atgtcgatct tctgcttcga agatggcatg tccagggtgt cgtgcgacgc
     1201 ggtcgtcgca ttgcggatcc tggtcaacat gtctgctatg gggtctgtga gtgacatctg
     1261 atcaccaact cgatttcgtg ataccgggta gttcgcctcg gtgagccaac tgtctgacgc
     1321 agagcctgca cataccaaac ttcctcaggt aaccacgggg acgaccacag cgacggcaac
     1381 gcgtgtaggc gcggaccttg aactttgggg tcctgttcgc cttcgctacg agtgctttct
     1441 tcgccatgtc ttctccgtcg ccttatagct gcttgaatgg gaaaccgaac gactgcaaca
     1501 acgcaaggcc ttgttcgtcg gtggtggcac tggtcaccag cgtgatgtcc atgccgcgca
     1561 ccttcgcaac cttgtcgtac tccatctcag ggaagatcag ctgctcggtg acaccgaagg
     1621 tgtagttgcc ccttccatcg aaccccttgg gcgatagccc acggaagtct ctgatacgag
     1681 gaagagccac cgacaacagc cgatcgaaga actcccacat ccgatcgcct ctcaaggtta
     1741 ccttggcccc gatcgcctgt ccctcgcgaa ccttgaacgc ggcgatcgac ttcttcgctc
     1801 ttgtgatcat ggccttctgc ccggtgagaa gggcaaggtc ggcggcggca tcgtcgagcg
     1861 ccttcgactc gcgcagaacg tcacctacgc ccatgtttat cgagatcttc agcaggcgag
     1921 gcacggccat gacgttgaga ctcaattcgg tcgcgagagt gggacgcacc tcggagaggt
     1981 acttcgtctt gagtcttggt atctcagtca tagttgccat cagagttcag cctcacactt
     2041 cttgcagatg cgcgtcttgg aaccatcctc gtgaacggtg aatccgaccc gagtcacgcc
     2101 acagttattg cagacgaggg tcacgttcga gaggtcaacg ggaagatcct tagtgatgat
     2161 gccaccctct tgaccgcctc ggctcccctt cgagggccgg atctt
//
LOCUS       DATDOZ010000180         2021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3022449, whole genome shotgun sequence.
ACCESSION   DATDOZ010000180 DATDOZ010000000
VERSION     DATDOZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022449"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1287
                     /gene="istA"
                     /locus_tag="VI541_03620"
     CDS             277..1287
                     /gene="istA"
                     /locus_tag="VI541_03620"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HLF70027.1"
                     /translation="MEIQALHRQGWNISDLAREFGLDRRTIASYLEAPVAPRYPKRIC
                     PAEFTAEVLGHVLRRLEGCKTIRATTLYREVVEIGYAGSYVSFARRIRLLRPVTEREA
                     EVRFETDPGVQTQIDWANLGLWPLGTEMVELKALVGILGFSRMPAIRFGVDKTRETAM
                     AQIPALLHDLGGVTKEVLSDRDPVLVIGSTSDGKAIFAPEWIDLAASLGVTPKACKPY
                     RAKTKGKVERMIREVKEDFLCWITGQGMPPQPSLYDYQELAARWATDIVGNHVHRTTG
                     RVVREAWTEEVDHLRAIPERLLRQAFGNTVAAANVIDLTRFRESGAIVEHRDLADYEG
                     GL"
     gene            1287..>2021
                     /gene="istB"
                     /locus_tag="VI541_03625"
     CDS             1287..>2021
                     /gene="istB"
                     /locus_tag="VI541_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HLF70028.1"
                     /translation="MNASAYQQLRSHLAYLGLSTASERLAHHLDAGGSATEIIEALLG
                     EEVEATMARKLASRLRFGHYPLRKTLAEFDFSFQPSIDRKVIGELSTLRFVEEKRNVL
                     LLGPPGVGKSHLAIGLGIAATEAGYRTYFTSAQDLVRGLATAHLEGRWASKMRSFTGP
                     SVLVIDELGYLPMDQASAHWIFEVVTRRYERGSIVLTSNRGFAEWGQVFADAVVASAI
                     LDRLLHHATVVNVKGKSFRMRSHASGF"
BASE COUNT          366 a          606 c          662 g          387 t
ORIGIN      
        1 agattccgtt ggtggcaaag atgagtgatg ctctcggaga cgccgcctca gtcgtgaacg
       61 atgtgggatc ggcaagatcc gattgaatgt tgggcggacg agcgatcgtg ttggcgatca
      121 tgaatagtgc tcaaccctga tcacgaaaag tgcgcaggcc ctcggggaga tccgggggct
      181 tcttcgttcc cggaaagagg tcgtccctcc cgccaggctc ggggtgtcta ggacccgatc
      241 ggcaggaggg acggagtgca cacagaggag atctggatgg agattcaagc actgcatcgt
      301 caaggatgga acatcagcga tctggcccgc gagttcggcc ttgatcgtcg caccatcgcc
      361 tcctacctcg aagcgcccgt cgctccgcgg tatcccaagc gcatctgtcc cgccgagttc
      421 accgcggagg tgctcggcca tgtgctgcgc cgcctcgagg gctgcaagac gatcagagcc
      481 acgacgcttt accgggaggt cgttgagatc ggctacgccg gcagctatgt atcgttcgct
      541 cgtcgcatcc ggctgttgcg tccggtcacc gaacgcgaag ctgaggtgcg cttcgagact
      601 gacccaggcg ttcaaaccca gatcgactgg gccaacctcg ggctctggcc gcttggcacc
      661 gagatggtcg agctcaaggc gctcgtgggg atcctgggct tcagccgtat gccggcgatc
      721 cgcttcgggg tggacaagac ccgtgagacg gccatggcgc agatccccgc gctgctgcac
      781 gacctcggcg gggtgaccaa agaggttctt tccgatcgtg atcccgtctt ggtaatcggt
      841 tccacctcgg acggcaaggc gatcttcgca ccggagtgga tcgacctcgc tgcatcgctg
      901 ggggtaacgc ccaaggcgtg caagccctat cgcgccaaga ccaagggcaa ggtcgagcgg
      961 atgatccgcg aggtcaagga ggacttcctt tgttggatca cgggtcaagg gatgccgccg
     1021 cagccatcgc tctacgacta ccaagaactc gcggcgcgat gggcaaccga catcgtcggt
     1081 aaccacgtgc atcgaacgac gggacgcgtc gttcgggaag cctggaccga agaggttgat
     1141 cacttacggg cgatcccaga aaggctgttg cgacaggcct tcggcaacac cgtcgctgcg
     1201 gccaacgtca tcgacctcac gcgcttccgt gagtctggcg cgatcgtgga gcaccgcgac
     1261 ctcgccgact acgagggagg gctctgatga acgcctctgc gtatcagcaa ctgcgttcgc
     1321 atctggcata cctcgggctg tcgaccgcct cggaacgact cgcgcatcac ctggacgccg
     1381 gtgggtcggc gacggagatc atcgaggcgt tgctcggtga ggaggtcgag gcgactatgg
     1441 cccggaagtt ggcttcgcgt cttcgcttcg gtcactaccc gttgcgcaag acgttggcgg
     1501 agttcgactt cagcttccag ccctcgatcg atcgcaaggt catcggtgag ctctcgaccc
     1561 ttcgcttcgt cgaggagaaa cgaaacgtgt tgctgctcgg gccaccgggc gtcggcaagt
     1621 cacatctggc catcggtctt gggatcgctg cgaccgaggc cggttaccgc acctacttca
     1681 cctcggcgca ggatctggtt cgcggcttgg cgactgcgca ccttgagggg cggtgggctt
     1741 ccaagatgcg tagcttcacg ggacccagcg tcttggtgat cgacgagctc ggctacttgc
     1801 cgatggatca agcctcagcg cattggatct tcgaggtcgt cacgcggcgc tacgagcgag
     1861 gctccatcgt gttgacgtcc aacagagggt tcgctgagtg gggccaagtg ttcgccgatg
     1921 ccgtggtcgc ttcggcgatc ttggaccggc tgctgcatca cgcgacggtg gtaaacgtca
     1981 agggcaagag cttccggatg cgctcacacg cgtcggggtt c
//
LOCUS       DATDOZ010000181         2793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_676094, whole genome shotgun sequence.
ACCESSION   DATDOZ010000181 DATDOZ010000000
VERSION     DATDOZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676094"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VI541_03630"
     CDS             <1..729
                     /locus_tag="VI541_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLF70029.1"
                     /translation="PEDVMSRAFDAGVVGVVTVGTDLESSRSSVALAEKFPAVWASVG
                     LHPHEASHLDEGSLDELRGLAAHPKVVAIGEIGLDYFRDHSPRADQRRAFDAQLSLAE
                     EIDKPVILHVRDAFEDVLDVLAGHSLSGIVFHCFSGGPLEASRAVAIGGYVSLAGNVS
                     YRTAQNLRDAAKATPLDRLLVETDAPYLAPVPMRGRPNEPAYVVHVGSALAIERRMPE
                     ADLAATTHRNAVRVFGITTRGRYG"
     gene            complement(704..2014)
                     /locus_tag="VI541_03635"
     CDS             complement(704..2014)
                     /locus_tag="VI541_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70030.1"
                     /translation="MRYRRAASLLASVLIGLVAFNSAFAAGSYPQYTTRPVPTSGQDA
                     FAVAVNGTTGYAFAAMPGSNTLSIIATDIKAEMQSLPMPDGPAGVAVNEVTNKIYVSS
                     YGNTGPTGHMLSIIDGNTFTRSEVDLGYNMGGYGVAVNATTNKIYVASFGSNSLAIID
                     GATLATSTVEFPTFTSIVPDIPVNEVPVCTSQPYAPTDTAYYCVNDVEPQPIGVAVNE
                     STNKVYVTGWGSNQIFVIDGASNALESTISDDTSPGGLQRISGPWGIAVNQAAQEVHV
                     AMPGNSRLSTIDTNPTHVSTYNKFIRCLGETGGTRPCTLGAQNGITTLAFDAPRNQLV
                     AAFYNQPGSVKVYNPATNWSSTSITAGVTTIGVGVNPSNGDIYSANPGNLDNWFKKLW
                     LNPSQGVRDHAGDPVGFLMDANSGSKWQSINPGFVSVSRNGPGL"
     gene            2247..>2793
                     /locus_tag="VI541_03640"
     CDS             2247..>2793
                     /locus_tag="VI541_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HLF70031.1"
                     /translation="MRVLLTLGFAACTTMYFMAEKQVTLADDGRVVKVKTFAPTVGAA
                     LVRLGVSVGPDDLVSPSPISDLSDHIEVRRARDIVLVLNGARSIERVTGSTQEEVLAE
                     LDLDRGSRLYSSRDDMADGDALVVNHPVSTHLIHDGIDQPVVTDVLTAGALLRQLHIQ
                     LGPEDRVLPSIVAHPSQQTPIE"
BASE COUNT          541 a          807 c          832 g          613 t
ORIGIN      
        1 cctgaagatg tgatgagcag ggcgttcgat gctggagtcg ttggggtcgt cacggtcggg
       61 acggatctcg agagcagcag atcctcagtg gctcttgctg aaaagttccc cgcggtgtgg
      121 gcgagcgttg gtctgcatcc gcacgaggca tcccatctag acgagggttc gctcgacgag
      181 ctgcgaggcc tcgcagcgca cccgaaggtt gttgccatcg gcgagatcgg tctcgactac
      241 ttcagggatc actcaccccg cgccgatcaa cgccgcgctt tcgatgctca gttgtcgttg
      301 gccgaggaga tagacaagcc ggtgatcctg cacgttagag atgcattcga agatgtctta
      361 gacgtgctgg caggccactc actctccggg atcgtcttcc actgcttctc cggtggacct
      421 ctggaagcct ctagggcggt ggccatcggc gggtatgtgt ctcttgctgg caacgtctcg
      481 tatcgcaccg cccagaacct tcgtgatgcg gcaaaagcga cgccactcga ccgactgtta
      541 gtagagaccg acgcgcccta cctggcccca gtccccatgc gcggcaggcc gaatgaaccg
      601 gcttatgtag tgcacgtcgg atcggcacta gcgatagaaa ggcgaatgcc cgaggcggat
      661 ctcgcagcaa ctacgcatcg caacgcggtt agggtgttcg gtattacaac ccggggccgt
      721 tacggctgac tgatacgaag cccgggttga tgctctgcca cttcgaaccc gagttggcat
      781 ccatgaggaa tccaaccggg tctcccgcgt ggtcgcggac gccctggctc gggttgagcc
      841 ataacttctt gaaccagttg tcgaggttgc ctgggttggc tgagtagatg tcgccgttcg
      901 aggggttgac tccgacacca atggtcgtga caccggcggt gatcgaagtg ctgctccagt
      961 tagttgcagg gttgtaaacc tttacgctgc ccggttggtt atagaaagcg gcgaccaatt
     1021 ggttcctcgg ggcatcaaaa gcgagcgtcg tgatcccgtt ttgggccccc agcgtgcatg
     1081 gtctggtgcc gcccgtttcg ccaaggcacc tgatgaactt gttgtatgtc gagacgtggg
     1141 ttgggttcgt gtcgatggtc gagagccgac tgttgccggg catcgcaacg tgaacctcct
     1201 gggccgcttg gttcacggct atgccccacg gtccgcttat ccgttgcaag ccgcctggag
     1261 aggtgtcgtc cgagatggtc gactccaggg cgttactggc cccatcgatc acgaagatct
     1321 gattggatcc ccagccggtc acatagactt tgttggtgct ctcgttgaca gccacgccga
     1381 tcggctgagg ctctacgtcg ttgacgcagt agtaagcggt gtcagtcgga gcgtaaggct
     1441 gactggtaca aaccggcacc tcattcactg ggatgtccgg cactatcgac gtgaaagtcg
     1501 gaaactccac ggtgctagtc gccaacgtag ctccgtcgat tattgccaag ctgtttgatc
     1561 cgaagcttgc cacgtagatc ttgttagtgg tggcgttgac ggccacgcca tatccaccca
     1621 tgttgtagcc gaggtcgacc tcgctacggg taaaggtgtt cccatcgatt atcgagagca
     1681 tgtgacctgt gggtccagtg tttccgtaag aggagacgta gatcttgttg gtgacctcgt
     1741 taacggcaac cccggctggt ccgtccggca tagggaggga ctgcatctcc gccttgatgt
     1801 ccgttgcaat gatcgagagg gtgttcgacc ccggcatcgc tgcgaaggcg tagccagtcg
     1861 taccgttcac cgctactgcg aaggcatcct gaccggaggt cggtacgggt cgggtcgtgt
     1921 actggggata gctacccgcg gcgaatgcag agttgaaggc caccagaccg atcaggacac
     1981 ttgcgagcaa cgaggctgca cgccggtacc tcaacaatcc tccctcgtcg aaccgatcga
     2041 tgcgtttgaa ccgacctgcc tccgagggca ttgagttcac aattcgacga accgtccgag
     2101 aaacctgctt gttccgcaaa aaatcttcgt ttgatctgac acttccgtat cttccacgcc
     2161 agacatccgg gccaggctcg cttaccacct gcccgtttgc ccatttggac gaaacgctgg
     2221 taggttgccc ccctgtgaga ggagtcttga gagtcctcct cactctcggt ttcgcagcat
     2281 gcaccacgat gtacttcatg gccgagaagc aggtgactct cgctgatgac ggccgcgtcg
     2341 tcaaagtcaa gacattcgca ccaacggtcg gcgcggcact ggttcgtcta ggagtaagcg
     2401 tcggtcctga cgaccttgtt tcgccatccc cgatttcaga tctgtcggat cacatcgagg
     2461 ttcgtcgtgc acgcgacatc gtactcgtgc tgaacggtgc gagaagtatc gaacgagtca
     2521 ctggttccac gcaggaagag gtcctggctg agttggacct cgatcgaggg tcacgactct
     2581 actccagcag agacgacatg gccgatggcg atgcactcgt tgtgaatcat ccggtcagta
     2641 cccacctgat acatgacggg atagaccaac ccgtagttac cgacgttctc actgctggtg
     2701 ctctgcttcg gcaactgcac atccagcttg gcccagaaga ccgagtgctc ccgagcatcg
     2761 tcgcccatcc gtcgcagcaa actccgatcg agg
//
LOCUS       DATDOZ010000182         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_676473, whole genome shotgun sequence.
ACCESSION   DATDOZ010000182 DATDOZ010000000
VERSION     DATDOZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676473"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2248
                     /locus_tag="VI541_03645"
     CDS             <1..>2248
                     /locus_tag="VI541_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HLF70032.1"
                     /translation="PGFLGVAMTFSWDGGDKHRGIIETACSREGIDVVAWREVPIDVG
                     VLGEAAALSAPSIEQAILLRPIGVDKAEAERRCIRARKHAEKDCRDAGLRVYFPSFSF
                     LTVTYKALCVADQLSSFYLDLAQPSFTAPFAIFHQRFSTNTLPTWERAQPFRTLCHNG
                     EINTIQGNINRMRAREGRLGKRNLLEEELLRPVIDESGSDSAMLDNVVELLSTEGRDI
                     SHVMAMLVPAAWENAEIEDEVRDFYRYHSCIVEAWDGPAGLIFTDGTKVGATLDRNGL
                     RPLRYSFSESGIVACSSEAGSVDLSGQGSITRRKLGPGEMIVVDPSNGGLIETPIQAL
                     ARAKPYGEWTERYLQASPLQDPDPKIPDDILQRQVAFGYTKEMFTTVIRPMATSGHEP
                     TSSMGDDTASAILSDHLRPLYNYFKQRFAQVTNPPIDPVRERAVMSMRTLIGPHDPIL
                     WERPEAASLIELDTFFLFKPLGGYPIDATFPVAAGARGLAEAIESIADEADQAARIGS
                     GILVLSDAAVSQERAPVPMLLAVGAVHQRLLRAGHRTRTSIVVETDEAREVHEFACLL
                     GYGAEAICPRLALASIADLTQKGRLGEDIPVSEALMRYRSAVEEGVLKILSKMGISTT
                     DSYRGAQIFDAVGLAQGLIDLCFTGTHSPFGGIGVDEIAQDVLRRHFAAYSVQSKMEN
                     PGYLKHHSKGEYHETNPGVVDALHSAVRSGSWDDYARFAALVEDRPVSEPRDLLQFKQ
                     TDPIALDEV"
BASE COUNT          441 a          708 c          668 g          431 t
ORIGIN      
        1 acccaggctt cctcggggta gcgatgactt tctcgtggga tggcggtgac aaacatcgcg
       61 gcatcatcga gacggcctgt tcacgtgaag gcatcgatgt cgtcgcatgg cgggaggtcc
      121 ccatcgatgt cggggtcctc ggcgaggcag ctgccctatc ggcccccagc atcgaacagg
      181 ccatccttct gcggcccatc ggagtcgaca aggccgaggc tgaacgccga tgcatcagag
      241 cgcgcaaaca tgccgagaag gactgcagag acgctggtct tcgcgtctac ttcccatcgt
      301 tctcgttcct cactgttacc tacaaggctc tatgcgtggc cgaccagttg tcttccttct
      361 atctcgacct ggcgcagcct tccttcactg cgcccttcgc catcttccat cagcggttct
      421 cgacgaacac ccttccgacc tgggagaggg cacaaccatt ccggaccctc tgccataacg
      481 gtgagatcaa caccatccag ggcaacatca atcggatgag agcccgtgag ggaaggctcg
      541 gcaaacgcaa cctgctcgag gaggaattgt tgcgacctgt gatcgacgaa tcggggtccg
      601 actctgcgat gctcgacaat gtagtcgagt tgctctcgac cgaggggcgc gacatctcgc
      661 acgtcatggc catgctcgtg ccagccgcgt gggagaacgc cgagatcgag gatgaggtcc
      721 gcgacttcta tcgttatcac tcctgcatag tcgaagcctg ggatggcccc gcgggtctca
      781 tcttcaccga cgggaccaag gtcggcgcga cgcttgatcg gaatggtttg agacccctcc
      841 gttacagctt ctccgagagc gggatagtgg cctgttcgtc cgaagcgggg tcagtggatc
      901 tgagcggtca gggaagcatc accaggcgca agcttggacc cggagagatg atcgtcgtcg
      961 acccgtcgaa cgggggcctc atcgagaccc cgatccaagc tctcgctagg gccaaaccat
     1021 acggcgagtg gaccgagaga tatctgcagg cgtctcctct acaggacccc gacccgaaga
     1081 tcccagacga catcctccag cgacaggttg cattcgggta cacgaaggag atgttcacga
     1141 cggtcatcag gcccatggcg accagcgggc atgaaccgac gtcttcgatg ggtgacgaca
     1201 cggcatccgc gatcctctcc gatcatctcc gtcccctgta caactacttc aaacagcgtt
     1261 tcgcccaggt aaccaatcca ccgatcgacc ccgtacgcga gcgggcggtc atgagcatgc
     1321 gaacactgat agggccccat gacccgatcc tctgggagcg cccagaggcc gcatcactca
     1381 tcgagctcga tacgttcttc ctcttcaagc ctctcggggg ctacccgatc gacgcgacat
     1441 ttccggtagc agcgggggct cgtgggctcg ccgaggcaat agagagcatc gcagacgagg
     1501 cagatcaggc cgctcgcatc ggtagtggga tcctcgtgct ctccgacgct gcggtgtcgc
     1561 aagagcgtgc accggtaccc atgttgctgg cggtcggcgc ggtccatcag cgcctgttgc
     1621 gagcgggcca tcgcacgcgc accagcatcg tggtcgaaac cgatgaggca cgtgaggtcc
     1681 acgagttcgc ttgtctgctc ggctacggcg ccgaggccat atgcccacga ctcgcccttg
     1741 cgtcgatcgc cgatctcacg cagaagggtc gtctcggtga ggacatccct gtaagtgagg
     1801 cattgatgcg ttatcggtca gccgttgagg agggagtcct caagatcctc tccaagatgg
     1861 gtatctcgac tactgactcc tatcgcggcg cgcagatatt cgatgcggtc ggcctcgctc
     1921 agggactgat cgacctctgc ttcacgggca cgcactcgcc cttcgggggc ataggagtcg
     1981 atgagatcgc tcaggacgtt ttgcgaagac acttcgctgc ctactcggtt cagtcgaaga
     2041 tggagaaccc cggctatctg aaacatcact ccaagggtga gtaccacgag accaacccgg
     2101 gcgtcgtcga cgcgcttcac tccgctgttc gcagcgggag ctgggacgac tacgcgcgct
     2161 tcgccgcgtt ggtcgaggac cgtcctgtca gcgagcctcg cgacctcctg cagttcaagc
     2221 agacagatcc catcgcgctc gatgaggt
//
LOCUS       DATDOZ010000183         5656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1736609, whole genome shotgun sequence.
ACCESSION   DATDOZ010000183 DATDOZ010000000
VERSION     DATDOZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736609"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /gene="atpB"
                     /locus_tag="VI541_03650"
     CDS             <1..453
                     /gene="atpB"
                     /locus_tag="VI541_03650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLF70033.1"
                     /translation="IPWVGFTPTSNIMVTGALAIGVYLLAISVGMARHGIFKFWVKTL
                     VLKDISKPLLILMVPIEIVSQLARPFSLAVRLFANMLADHVILLIFVGFIFLAGAKFG
                     IGHVIIIPISMVFEIVFTFFALFVAFIQAVIFAFLSAIYINDALHPGH"
     gene            482..763
                     /gene="atpE"
                     /locus_tag="VI541_03655"
     CDS             482..763
                     /gene="atpE"
                     /locus_tag="VI541_03655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HLF70034.1"
                     /translation="MQDALVLAQEAAKSGITNTGLALLGGGLAIGLGAGGGAVGIGIL
                     WSKGLEGIGRQPEARGTLQGLMFLGFALAEAQVLYAFVVTILLLFVLKG"
     gene            774..1310
                     /gene="atpF"
                     /locus_tag="VI541_03660"
     CDS             774..1310
                     /gene="atpF"
                     /locus_tag="VI541_03660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HLF70035.1"
                     /translation="MTHSIVLAAEGFNPLKPDPGLFVWVTIAFLVVLFVLAKKVFPKL
                     QESLADREAKIGGDIAEAERIKQEAEAVLSDYKARVAQAREEANKILEETRQSADSVR
                     KDLIAKSEGEARLIVDKAQKELAGERERTIKAIQSQLAEWSTNIASRIVERELSPEGQ
                     SHLVEAFIADLEKESQGS"
     gene            1307..1843
                     /gene="atpH"
                     /locus_tag="VI541_03665"
     CDS             1307..1843
                     /gene="atpH"
                     /locus_tag="VI541_03665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HLF70036.1"
                     /translation="MKDARIAGYAKALFEIASTENQIDRVADELFRIARTLEAENDLR
                     QTLTDIAIPVETKDKLLSDILGAKVSTHTLGILRFVIGQGLARDLVGIADELAALAEQ
                     ESRREIAEVRSAVAMSDDQITRLGAALGKATGKDVTVKVIIDPSVIGGVLARVGDVVI
                     DGTVRNKLQSLKEHLGVR"
     gene            1871..3391
                     /gene="atpA"
                     /locus_tag="VI541_03670"
                     /pseudo
     CDS             1871..3391
                     /gene="atpA"
                     /locus_tag="VI541_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            3454..4329
                     /locus_tag="VI541_03675"
     CDS             3454..4329
                     /locus_tag="VI541_03675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HLF70037.1"
                     /translation="MAEKARDITRRIRSIASTKKITNAMELIAASRIVKAQTRLEKSR
                     PYSRAISEMIEMLASEAGSSPLLAEREAHNVAIIVITGDRGLAGAYNSNVLREAQRAA
                     GHERELGRNVVTTTVGRKGASYLSFRKVPLAASFRGMSDAPAYADAKQVAEGVIAAFT
                     AGEIDKVIIAYTEFVSAFLQRARVAQILPVQRSEVREQAPSGPVAVFDFEPEPEELLQ
                     ALLPRYVEIRMYSCMLESSASEHAARRRAMKNATDNAQELIDHYRLRANKARQAEITN
                     EIADIVGATEALRGA"
     gene            4355..>5656
                     /gene="atpD"
                     /locus_tag="VI541_03680"
     CDS             4355..>5656
                     /gene="atpD"
                     /locus_tag="VI541_03680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLF70038.1"
                     /translation="MATTAQDMLATANQGRVISVIGPVVDVEFPPDSLPEINHALVIE
                     RELGGKRDDITAEVAAHVGHSMVRAICMKPTDGLIRGAKVRDTGAPISVPVGPKVLGH
                     VFDVLGEPLDVAASEFDDVERWAIHRDPPPFDELEPKTEMLQTGIKVLDLLQPYVKGG
                     KVGLMGGAGTGKTILIKEMIYRIGQEHGGYSVFAGVGERTREGNDLWIEMNEAGPDVI
                     SKVAMVFGQMDEPPGVRLRVGLSAITMAEYFRDVEHRDVLVFIDNIFRFVQAGSEIST
                     LLGRMPSAVGYQPTLSDEMGQLQERITSTKGRSITSIQAIYVPADDFTDPAPHSTFAH
                     QDATTVLSRELFALGIYPAIDPLASFSRVLDARYVGQEHYDVANEVKRILQRYKDLQD
                     IIAILGIDELSEEDKLDVYRARKIQKFLSQPFYVGEVFIGIP"
BASE COUNT         1130 a         1698 c         1731 g         1097 t
ORIGIN      
        1 atcccgtggg taggctttac gcccacctcg aacatcatgg tcacgggtgc gctagcgatc
       61 ggggtctatc tgcttgccat cagcgtcggg atggcccggc atggcatctt caagttctgg
      121 gtcaagaccc tcgtcctgaa ggacatctcc aagccgctgc tgatcctcat ggtcccgatc
      181 gagatcgtct cgcagctcgc acggcccttc tcattggccg ttcgtctctt cgccaacatg
      241 cttgccgacc acgtcatcct gctgatcttc gtcgggttca tcttcctggc gggggcaaag
      301 ttcggcatcg ggcatgtgat catcatcccg atctcgatgg tcttcgagat cgtcttcaca
      361 ttcttcgccc tgttcgtagc attcatccag gccgtcatct tcgctttctt gtcggccatc
      421 tacatcaacg atgcgcttca cccaggtcac tagcactcat aggtactgaa aggagaacaa
      481 cttgcaagac gcattggtat tagcgcagga ggcagcgaag tcgggcatca ccaacacagg
      541 tctggccctt ctcggtggtg ggcttgcgat cggtctgggg gccggaggcg gcgccgttgg
      601 tatcggcatc ctgtggtcca agggtctcga aggtatcggg cgtcagcctg aagcccgcgg
      661 caccctgcag gggctcatgt tcctcgggtt cgcattggcg gaagctcagg tcctctacgc
      721 attcgtcgtc accatccttc tcctcttcgt gttgaagggt taagggggag gtcatgacac
      781 actccatcgt tctggcggct gaggggttca accccctgaa acccgaccca ggcctattcg
      841 tctgggtgac catcgctttc ctggtggtcc tgttcgtcct ggccaagaag gtcttcccga
      901 aactgcagga gtccctcgcc gatcgtgagg ccaagatcgg cggcgatatc gccgaggccg
      961 agaggatcaa gcaggaggcc gaagcggtgc tcagcgatta caaggcccgc gtggctcagg
     1021 cccgcgaaga ggcgaacaag atcctagaag agactcggca gtctgccgac tcggtccgca
     1081 aggatctgat cgcgaagtct gaaggcgagg cccgcctgat cgtcgacaag gctcagaaag
     1141 agttggcggg cgagcgcgag aggaccatca aggcgatcca gtcgcagctt gccgagtggt
     1201 cgacaaacat cgcttcgcgc atcgtcgagc gcgagctctc accagagggt cagtctcacc
     1261 tagtcgaggc attcatcgcc gacctcgaga aggagagtca aggctcgtga aggacgcgcg
     1321 catagcgggt tacgcgaagg ctctcttcga gatagccagc actgaaaacc agattgaccg
     1381 tgttgcggac gagttgttcc gcatagcgcg caccttagag gccgagaatg atctgcgaca
     1441 gacgctcacg gacatcgcca tacctgttga gaccaaggac aagctcctct ccgacatcct
     1501 gggggccaag gtctcgaccc acactctggg gatcctgcgt ttcgtcatag gccagggtct
     1561 cgcgcgagat cttgtgggta tagccgacga actcgctgct ctcgccgagc aggagtcgcg
     1621 gcgcgagatc gccgaggtcc gctcggccgt cgccatgagc gacgatcaga tcacgcgact
     1681 gggggccgca cttggcaagg cgactggcaa ggacgtcacg gtcaaagtga tcatcgaccc
     1741 aagtgtcata ggcggtgttc tagcaagggt gggcgatgtc gtgatcgatg ggaccgttcg
     1801 caacaagttg cagtcattga aggaacacct gggagttcgc taacccattt acaagacata
     1861 ggagttgctg atgccagatc tgaccataga gcccggagag atccgttcag ctctcgacgc
     1921 gatgttcgcg tccttcaagc cgacgatcga cctagaggag gtgggtagcg tcaccaacgc
     1981 cgcagatggt gtcgccatcg tttcggggct tccgcacact atggccaacg agatgctcga
     2041 attccccggc ggagtgatag gcatcgccct gaacctcgag gccgatcaga tcggtgtcgt
     2101 tctcttggga gatgtgtcgg ggatcgaaga ggggatgccc gttcgccgga caggaaaggt
     2161 gctctcgatc cctgtgggag atgcctatct cggtcgcgtc gtcgaccccc tgggccagcc
     2221 gctcgacggt aagggccccc tcgactcatc caagatcgac ggcactcgtg agttggagtt
     2281 gcaggctcca tcggtcatca atcgccagtc ggtcagcgaa ccgctgcaga ctggcatcac
     2341 ggcgatcgac gcgatcaccc cgatcgggcg cggtcagcgc gaactgctga tcggcgaccg
     2401 tcagaccggt aagactgccg tggcaaccga cacgatcatc aatcagcggc agtactgggg
     2461 taccgacaag gccgtcaagt gcatctatgt cgccatcggt cagaaggcat cgaccgtggc
     2521 cgaggtcgtg aacgggctct ccgaggctgg ggcgatggag tacacaaccg tcatcaatgc
     2581 ccccgcatca tggcccggcg ctttcaagtt cttggccccc tactcgggct gcgcgatcgg
     2641 tcagtactgg atgtacaagg gtgagcatgt gctcatcgtg tacgacgacc tttcgaagca
     2701 ggccgaggca taccgtcaga tcgcgctgct actgcgcagg cctccgggtc gcgaggccta
     2761 tccgggcgac gtcttctacc tgcattcacg tcttctggag cgcggcgcga agctctctgc
     2821 ggagttcggc ggcggttcga tgacggctct tccggtcatc gagaccaagg agggtgacga
     2881 aactgcttac atcccgacca atgtcatctc gatcacagat ggtcagatct atttcggcaa
     2941 ggacctcttc ttccagggtc agcgaccggc ggttaacgtc ggtacgtcgg tctcgcgagt
     3001 tggtggcgcc gctcagatca aggcgatgaa gcaggtcgcg gggcccccca agatccagct
     3061 cgcccagtac cgagagttgg cggcattcgc agcattcgcg tcggacctcg acaaggcctc
     3121 gcgtgaccag ctcaaccgag gcgcgcgcct gaccgagatc ctcaagcaac cacagttcga
     3181 gccgatccca gtcgagagac aggtcgtatc tgtctttgcg gggatgaacg ggtacctgga
     3241 cgacatccct cttccggagg ttcgcagatt cgaccgcgag ctactcgaag ctatcgcgac
     3301 aagagcgcca caggtgtacg agcacatcca gaccaagggg gacttcccag aggatatcga
     3361 gaaggcactg cgcgttgcga tcgaggagtt cgcgctcacc ttcacgccgt cggttgccac
     3421 tcccaaagtc agcacggggg cctagcgcaa catatggccg agaaagcaag ggacatcacc
     3481 cggcgtatcc gctccatcgc gtccacgaag aagatcacca acgctatgga actgatcgcc
     3541 gcgagccgga tagtcaaggc gcagactcgc ctggagaagt cgcgtccgta ctcgcgtgcg
     3601 atcagcgaga tgatcgagat gctcgcgtct gaagcgggct catcgccact tctagccgag
     3661 cgcgaggccc acaacgtagc gatcatcgtg atcacgggcg accgggggct cgcgggggcc
     3721 tataactcaa atgtccttcg cgaggcccag cgcgcggctg gtcatgagag agagcttggt
     3781 cgcaacgtcg tcaccacgac cgttgggcgc aagggtgcaa gttacctctc cttcagaaag
     3841 gtcccactgg ccgctagctt ccgcggcatg tccgatgctc ccgcgtacgc cgatgccaag
     3901 caggtggccg agggcgtcat cgcggcattc accgcaggtg agatcgacaa ggtcatcatc
     3961 gcttacacgg agttcgtctc ggcattcctg cagcgcgccc gtgtcgcaca gatcttgccg
     4021 gtgcagcgct cggaggttcg ggagcaggcg ccaagtgggc ctgtggctgt attcgacttc
     4081 gaacccgaac cagaagagtt gctgcaggca cttcttcctc gctacgtcga gattcggatg
     4141 tactcgtgca tgctcgaatc ttcggcatcg gagcatgccg ctcgtcgaag ggcgatgaag
     4201 aacgcgacgg acaacgcgca ggagctcatc gaccactacc gattgagggc caacaaggca
     4261 cgccaagcag agataacgaa cgagatcgca gacatcgttg gggccacaga agcactgcgt
     4321 ggcgcttgag tccaaaggga gtgaggtaat cacaatggct acaactgcgc aggacatgtt
     4381 ggcaacagcg aatcagggtc gcgtcatatc ggtgatcggg cccgtggtag atgtcgaatt
     4441 cccgccggac tctttgcctg agatcaacca cgcgctggtt atcgaacgcg agctgggtgg
     4501 caagcgcgac gacatcaccg ccgaggtggc cgcgcatgtg gggcactcga tggtgcgggc
     4561 gatctgcatg aagccgaccg acggcctcat ccgcggcgcg aaggtccgcg atacaggggc
     4621 ccccatctcg gtacccgtag gccctaaggt ccttgggcat gtgttcgatg tgcttgggga
     4681 accgctcgat gtcgcggcct cggagttcga cgacgtcgag cgctgggcca tccaccgcga
     4741 tcctccgcca ttcgatgagt tggaacccaa gaccgagatg ctccagaccg gcatcaaggt
     4801 cctcgaccta ctccagcctt acgtcaaggg gggcaaggtc ggcctgatgg gcggcgcggg
     4861 gaccggcaag acgatcctga tcaaagagat gatctaccgg atcggtcagg agcatggtgg
     4921 ttactcggtg ttcgccggtg tgggtgagcg tacccgtgag ggcaacgacc tttggatcga
     4981 gatgaacgag gccggtcccg acgtgatcag caaggtcgcc atggtgttcg gtcagatgga
     5041 tgagccgccg ggcgttcgtc ttcgcgtcgg tctatcggcc atcacgatgg ccgagtactt
     5101 ccgtgacgtc gagcaccgtg atgtgctcgt tttcatcgac aacatcttcc gattcgtgca
     5161 agcggggtcc gagatctcga cattgctggg acgcatgcca tctgctgtgg gctatcagcc
     5221 gacgctctcc gacgagatgg gtcagttgca ggaacggatc acctcgacga agggtcgttc
     5281 gatcacctcg atccaggcga tctacgttcc cgctgatgac ttcaccgacc ccgcccccca
     5341 ctccacgttc gctcaccagg atgcaaccac ggtcctctcg cgagagctct tcgcgcttgg
     5401 catctacccg gctatcgacc cgctcgcctc gttctcacgt gttctcgacg cacgctacgt
     5461 tggtcaggag cactacgacg tggcgaacga ggtcaagcgg atcctccagc gctacaagga
     5521 tctgcaggac atcatcgcga tccttggcat cgatgaactc tccgaagagg acaagctcga
     5581 cgtctaccgc gcccggaaga tccagaagtt tctctcgcag cccttctatg tcggcgaggt
     5641 gtttatcggg atccca
//
LOCUS       DATDOZ010000184         3030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3549865, whole genome shotgun sequence.
ACCESSION   DATDOZ010000184 DATDOZ010000000
VERSION     DATDOZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549865"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..889
                     /locus_tag="VI541_03685"
     CDS             17..889
                     /locus_tag="VI541_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HLF70039.1"
                     /translation="MHKHFGNTAVGEIDLDRFNPSRYLEDFDGGRVSQLSDGRTLREF
                     DITAVDVEIEVAPGVKFSAWAYNGRVPGPTLRATQGDVVRINFRNGGSHPHTMHFHGF
                     HAAGMDGVFELVRKGESFTYQFTAEPFGLHLYHCHTPPLKRHVHKGLYGVFIVDPPGG
                     RPPAHELVMMMNGFDTNFDGSNEFYAVNTVAHHYVRHPIRVKRNEPIRVYLVNITEFD
                     LINSFHLHANFFHEYRTGTKLEPDNFTDTIILGQGERSILEFAYRDPGTYMFHAHVSE
                     FAELGWMGMFEVEG"
     gene            886..2052
                     /locus_tag="VI541_03690"
     CDS             886..2052
                     /locus_tag="VI541_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HLF70040.1"
                     /translation="MNPGRTRRLMFVMVPVLVIVALAAFLIKAGPKGLFPGEQFPEES
                     ITFGRITLTPNHIRIHVTNGGAPTVTVAQVAVDSALWEFEASPSATITRLRSADIDID
                     YPWVTGEPVQLALITSTGLQFEHEIAAATTTPQRNGRFVLTFALLGLYIGIIPVLAGM
                     TLLPFLRTLRARWIHISLAFSAGILIFLGAETLVDAIERSRDLPNALGGTGLVVFAAA
                     VSFLGILAGSRWLKSRSTSDARLVTAVLVAAGIGLHNLGEGLAVGAAYRLGEIGLGTF
                     LVIGFAIHNSTEGLGIVSLLTDRKPSLMLLGGLGLLAGGPTILGAWTGAFFFSPTIAA
                     AFLAIAAGAIAEVVVDVLSVVRKESEGGLASAPILIGVAAGVAVMYLTGLLVTA"
     gene            2049..2417
                     /locus_tag="VI541_03695"
     CDS             2049..2417
                     /locus_tag="VI541_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF70041.1"
                     /translation="MRPLASLAMGGLSILIACTPAPSPLVERGERLATERGCVSCHST
                     DGSAGIGPTWKGLAESQVELTSGEVVLADDAYLRESMTDPSAKTVRGFKTGLMERVIA
                     PGSLSDSEVRALIAYVKSLR"
     gene            2446..>3030
                     /locus_tag="VI541_03700"
     CDS             2446..>3030
                     /locus_tag="VI541_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLF70042.1"
                     /translation="MPDDRAYERSAERTHIQRTALLSLGAAIVLIVIKLAAGLASGSL
                     AILSEAAHSALDAGATGITYFAVRIASRPPDEDHPYGHGKAENISALIETFGLLGLSA
                     FIGVQAIARLQGAGPHPRATWYGFAVIGASIVIDFSRSRALGRAGRKYHSPALQADAL
                     HFTADLLTSSIVLIGLILLRFGLTQADAIGSLLIA"
BASE COUNT          553 a          997 c          891 g          589 t
ORIGIN      
        1 ggagaaggtc agggacatcc acaagcactt cggcaacacc gccgtcggtg agatcgacct
       61 cgaccgcttc aacccgtcgc gctacctcga agacttcgac ggaggccgcg tgagccaact
      121 ctccgacgga aggaccctgc gcgagttcga cataaccgcg gtggatgtcg agatagaggt
      181 cgcacctggg gtgaagttct cagcttgggc atacaacggg cgcgttcccg gcccgaccct
      241 gcgcgcaacg caaggcgatg tcgttcgcat caacttccgc aatggcggtt cgcaccccca
      301 cacgatgcac ttccacggtt tccacgcagc cgggatggac ggcgtcttcg aactcgtccg
      361 caagggcgag agcttcacct accagttcac agccgagcca ttcggactcc acctgtacca
      421 ctgccacacg ccaccgctga agaggcatgt ccacaaaggc ctctacggcg tcttcatcgt
      481 cgacccccca ggtgggcgac cccctgcaca cgaactcgtg atgatgatga atggattcga
      541 cacgaacttc gacggcagca acgaattcta cgcggtcaac accgtggccc accactacgt
      601 ccggcacccc atccgcgtga agcgcaacga gccgatccgc gtctacctgg tcaacatcac
      661 tgagttcgac ctgatcaact cgttccacct tcatgcgaac ttcttccacg agtaccgcac
      721 cgggaccaaa ctcgaaccag ataacttcac cgacaccatc atcctcgggc agggtgagcg
      781 ctcgatcctt gagttcgcct atcgagatcc agggacctac atgttccacg cacatgtctc
      841 tgagttcgcc gaactgggtt ggatggggat gttcgaggtg gaaggatgaa ccccggccga
      901 acgcggcgac tgatgttcgt gatggtgccg gtcctggtga tcgttgctct cgccgcgttc
      961 ctcatcaagg cggggccaaa gggcctcttc ccgggcgagc agttccccga ggagagcatc
     1021 acgttcggcc ggatcacgct gacgcccaac cacatacgca tccacgttac gaacggtggg
     1081 gcccccaccg tcaccgtggc tcaggtagcc gtcgactccg ccctctggga gttcgaggcg
     1141 tcaccatcgg caacgatcac gcgactgcgc tcggcggaca tcgacatcga ctatccatgg
     1201 gtaaccggcg agccagtcca actggcactg atcacctcaa ccggtctgca gttcgaacac
     1261 gagatcgcag ctgcaaccac gacccctcag cgtaacgggc gcttcgtcct caccttcgcg
     1321 ctgttgggtc tctatatcgg gatcatcccg gtgttggcgg gcatgaccct tctgcccttc
     1381 ctgcggacgc tgcgcgcgcg atggatacac atctcactcg cgttctcggc ggggatcctc
     1441 atcttcctgg gggccgagac tctggtcgac gcgatcgaaa ggtcacggga tctccccaat
     1501 gccctggggg gaaccggcct tgttgtcttc gcggcggcgg tgtcattcct tgggatcttg
     1561 gctggttcga ggtggctcaa gagccgcagt acatcagacg ccaggctagt cacagcggtc
     1621 ctggttgcgg cggggatcgg tctgcacaat ctcggcgagg gattggctgt gggcgcggcc
     1681 taccggttgg gtgagatcgg tctcgggact tttcttgtga tcggtttcgc catccacaac
     1741 tccaccgagg ggctcggcat cgtgtcactg ttgaccgacc gcaagcccag cctgatgctc
     1801 ctcgggggtc tgggtctgct ggctgggggc cccacgattc tcggcgcgtg gactggagcc
     1861 ttcttcttct cgcccaccat cgctgcggcc ttcctcgcca tcgccgcagg ggcgatcgcc
     1921 gaggtcgtcg ttgatgttct gagtgtcgtg aggaaggagt ccgaaggagg gctagcctcg
     1981 gccccgatac tcatcggcgt agcggctggt gtggccgtca tgtatctgac aggcctgctc
     2041 gtaactgcat gagacccctc gcgtctctcg ccatgggggg cctttcgatc ctcatcgctt
     2101 gtaccccggc cccctcgccg ttggtcgagc gcggagagag gttagcgact gagcgcggat
     2161 gcgtaagctg tcattcaacc gatggttcgg cgggtatcgg tccaacatgg aaagggctgg
     2221 ccgagtcaca agtggaactc accagtggag aggtcgtact cgccgatgat gcatatctgc
     2281 gtgaatcgat gactgacccg agtgccaaga cagtgcgcgg attcaagacc ggcctgatgg
     2341 agagggtcat cgcgcccgga tcgctctccg actcagaagt acgagcgctc atcgcatacg
     2401 tgaaatctct gcgatagcaa cgaggcctaa cctagacttg gggtcatgcc agacgaccgc
     2461 gcatatgaac gatcagccga gcgaacccac atccagagga cggcactgct atccctgggg
     2521 gccgctatcg tcctcatcgt tatcaagctc gccgcgggtc tggcctctgg cagcctagcc
     2581 atcctctcgg aagcggcgca ctcggccctg gacgccgggg ccaccgggat cacctacttc
     2641 gcagtccgca tcgcatctcg gcccccggac gaggaccacc cttatggcca cggcaaggcc
     2701 gagaacatct cggcgctcat cgagaccttc ggtctgttgg gcctatccgc cttcatcggt
     2761 gtgcaggcga tcgcgcggct tcaaggtgcg gggccccacc cgcgcgcaac gtggtatggc
     2821 ttcgcggtca tcggcgcgtc catcgtcatc gacttcagcc ggtccagggc cctcgggcgc
     2881 gctggccgca agtaccacag cccggcactt caggccgacg cccttcattt cacggccgac
     2941 ctcctgacct cttcgatcgt gctgatcgga ctgatcctcc tcagattcgg cctcactcaa
     3001 gctgatgcca tcggcagtct tctcatcgca
//
LOCUS       DATDOZ010000185         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_683491, whole genome shotgun sequence.
ACCESSION   DATDOZ010000185 DATDOZ010000000
VERSION     DATDOZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683491"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /locus_tag="VI541_03705"
     CDS             <1..1720
                     /locus_tag="VI541_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70043.1"
                     /translation="GGEEMSKFKGSDLVTVAHRIKQVAIATAILGGIGFAAARVIPND
                     TPSRAPAWSRWEPETSAVAGEYQDRAGSISQNDPTWLFLSDSPNSLASSPILAEYRSG
                     GIRLRGGSSTDPGGDTQEQGDPGLLDPAPVTVPKVPPLPPLPTIPPVLPSPTPSPSPT
                     PTPTPSPTPSPTPSPSPTTDPTEDPSDPPAVAVPDLGPSIEMINDIANQAQADAIYEV
                     TQTAGSGPVTAVSQAISEAIDQIQAFIADPAGTIDELVGPFLEGIRDLLPIGDPYADT
                     VAPAVESAWTTLDELGLTGQSYIDQVTGTVAATEAETVEGALATRDELLKGAPSQSDI
                     DEALDGVTQTVGGYYKTVSDATSGASDSARNQIDQTTKAVQDQADATQKQAGDTIANP
                     PPPPSLQKVTTAIVVPSNAMDGVNYYLLAGHTYKLVASGTYKWAPEQSLESEADALCS
                     TGFADPTWMANRFGNSILQLNLDNYLDVKGAPWTALSGSGCDPDHRYTFTFTALGDST
                     LNLRISDPDQLLNAGALTVTITDTFPKAIGSDAAGPVGTLFVITALLQGAVTRLRRFR
                     LNRDIG"
     gene            1742..3490
                     /locus_tag="VI541_03710"
     CDS             1742..3490
                     /locus_tag="VI541_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HLF70044.1"
                     /translation="MKVCESDMTIKDYLAVIRQRLIVVIMTIVITTGSAMVLAIRQTP
                     EYTATSTLRVYTVSPDVGGNKPFQDFSAIPKNIATEAEVVKSVEVAHLVIKRLHLDTR
                     KEEFGPENLSKSVTVVIKPYAEVLDVSVVASDPSLSSDLANAFSEEYIRFNRDQAMKE
                     NEKGASQIEDKIVALQNDYGQLVVESEGVAPSSDRAKLLETRKSQLIIKLAYLDDVRR
                     DFIARRDNNERIGTILAYAKPPQSRSNGDLARTGALGFIIGLPLALGLALILDAMGDT
                     IRTKEEAERIVGCETLALIPLTAEWKGSEPYVVSKEAPYSAAAEAFRTLRINLDSRGP
                     SGETKHYLITSPGIGEGKTTTSANLAVAFAEAGRSVLAVSADLRRPKLHAMFDLASGP
                     GLVEILRGQGESIEYVKEPSPNLYVLPSGSGADRPDQLLNRTDLRVMLDELSYVKSTS
                     RRPKPRNDENGAPKAGNGSGAGTRSRSTKVMVQPDTVLFDAPSILGAAEVSSLASAVD
                     GVVMILHPGITRRGAASRAAEQIRRAGGKIVGIVMVGVRVDDDYSVYTPVEDPDDDGG
                     RPTDSPWSRVVSSLKS"
     gene            3619..>4465
                     /locus_tag="VI541_03715"
     CDS             3619..>4465
                     /locus_tag="VI541_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70045.1"
                     /translation="MKVLSVVSSLPYPPDDGTRSTAWNFIRALNSIADVTVCTWVTGK
                     EDPDHIAQVRSAVKELITLADRGGRPTWPERIEFRARSLMLRVPPYILEKRDRCDPMP
                     ELDGFDLAVAEEDAALMLMPPFRCPVVVHRHNIFPDTIRTLRSSGALGTARKLKWISE
                     MPMWRRYNRELSEKATVSIATTPEAAESLRRVLPGVDVFVVCNGVKIPEEQLIPGPQP
                     RGIFTGVMDYEPNADAATSFVRDLWPRIVSEVPDASFQIIGRNPLKKVLRLSGQGVDV
                     VGGVPD"
BASE COUNT          956 a         1355 c         1316 g          838 t
ORIGIN      
        1 agggggggaa gagatgtcta agttcaaggg aagcgatctg gtaaccgtgg cccatcggat
       61 caagcaggtc gcgatagcta ccgcgatcct tggagggatc ggcttcgctg ccgcaagggt
      121 catccctaat gacacgccat ctcgagcgcc agcgtggtca cgctgggaac cagagacctc
      181 tgccgtggcc ggagagtatc aggatagggc tggcagtatc tcccagaacg accctacctg
      241 gctcttcctt agcgatagtc caaactcact tgcctcctct cccatcctgg cggagtaccg
      301 gtccgggggg atccgccttc gtggtggtag ctcaacggac cccggcggcg acacgcagga
      361 gcagggcgac cccggcctat tggatccggc ccccgtgacg gtgccaaagg ttccgcctct
      421 cccgcctctt ccgacgattc ctccggtgct gcccagccca acacccagcc catcgccaac
      481 acctaccccg acacccagcc cgacacccag cccgacaccc agcccatcgc caacaaccga
      541 tccaactgag gacccatccg atccgccggc ggtggcggtg cccgatcttg gcccttcgat
      601 cgagatgatc aacgacatag ctaaccaggc acaagctgac gctatatatg aggtcactca
      661 gaccgccggg tctggacccg ttacagcagt cagccaggcc atctcggagg ccatcgatca
      721 gatccaggca ttcatcgccg atcccgctgg gaccatcgac gagctcgtcg gtcccttcct
      781 agaggggatc cgcgatctct tgcccatcgg cgacccatac gctgacaccg ttgctccagc
      841 tgtcgagtcc gcgtggacca ctctcgatga acttggtctg accggccaga gctatatcga
      901 ccaggtgacc ggtactgtgg ccgcgaccga agctgagact gtcgaaggag cccttgcaac
      961 gcgggatgag ttgctgaagg gggccccatc tcagtccgac atcgacgaag ccctcgatgg
     1021 tgtcactcag acggtcggcg gatattacaa gaccgtttcg gatgccactt cgggtgccag
     1081 cgactcggca aggaaccaga tcgaccagac cacgaaggcc gtgcaggatc aagccgacgc
     1141 cacgcagaaa caggccgggg acacgatcgc caatcctcct ccgccgccct ctttgcagaa
     1201 agtgacgaca gcgatcgtgg tgccctcgaa tgccatggac ggtgtcaact actaccttct
     1261 cgctggtcac acatacaaac ttgtggcgag tgggacgtac aagtgggccc ccgagcagag
     1321 tctcgagagt gaagctgatg cactctgttc gacgggattc gcggatccga catggatggc
     1381 caacaggttc ggtaacagca tcctgcaact caacctggac aactacctcg atgtgaaggg
     1441 tgcgccgtgg accgctctgt ccggttctgg gtgtgacccg gatcaccgat acacgttcac
     1501 gttcacagca cttggtgata gcactttgaa cctaaggatc tccgacccag accagctgct
     1561 caacgcggga gcgctgaccg tgacgatcac cgacacattt cccaaggcga tcggttccga
     1621 cgcagcaggt ccggtaggga cgttgttcgt gataactgcg cttctccagg gtgcagtaac
     1681 taggttgcgc agattccgct tgaaccgaga catcggctga aggcgttgct gacgggaaag
     1741 agtgaaggtc tgtgagagcg atatgaccat caaagattat ctagcggtta tccgccaacg
     1801 actgattgtc gtgatcatga cgatcgttat cacgacgggg tcggcgatgg tccttgccat
     1861 caggcagacg cccgagtaca cggccacatc gactctgcgt gtctacaccg tcagcccgga
     1921 cgtaggcggg aacaagcctt tccaggactt ctcggccata ccgaagaaca tcgcgaccga
     1981 agccgaagtc gtcaagagtg tcgaggttgc acaccttgtc atcaagcggc tgcacctcga
     2041 cacacgcaag gaggagttcg ggcccgagaa cctgtcgaag agtgtcaccg ttgtcatcaa
     2101 gccctatgca gaggtgctag atgtgagcgt cgtggcatcc gacccaagcc tctcgagtga
     2161 tcttgcaaac gccttctctg aggagtacat ccgattcaac agggatcaag ctatgaagga
     2221 gaacgaaaag ggggctagcc agatcgagga caagatcgtc gctctgcaga atgactacgg
     2281 tcagttagtc gttgagtctg agggtgttgc gccttcgtcg gatagggcaa aacttctcga
     2341 gacccgcaag agccaactga tcatcaagct agcgtatctc gacgatgttc gtcgagactt
     2401 catcgctcgc cgcgataaca acgagcggat cggcacgatc ttggcttacg cgaaacctcc
     2461 tcagagccga tcgaacggcg acctagcgcg cacgggtgct ctcggattca tcatcggact
     2521 gccactcgcc ctgggcctgg cgctcatcct agatgcgatg ggtgacacga tccgaacgaa
     2581 ggaagaggcc gaacgcatcg ttgggtgtga aaccctggcc ctgatccccc tcaccgcgga
     2641 gtggaagggg agtgagccct acgttgtgag caaggaagcc ccgtactcgg cggcagctga
     2701 agctttccga acgctgcgga tcaaccttga ctctcgcggg ccgagcggcg agaccaagca
     2761 ctacctcatc acaagccccg gtatcggcga aggcaagacc actacgagtg caaacctagc
     2821 ggtcgccttc gccgaagccg gacgaagcgt gctcgctgtc tccgctgacc ttcgcaggcc
     2881 gaaactccac gcgatgtttg atcttgcttc cgggcccggc ctggtcgaga tcctgcgtgg
     2941 tcagggtgaa agcatcgaat acgttaagga gccgagcccc aacctctatg tccttcctag
     3001 cggaagcgga gcagatcgtc ctgaccagct actcaaccgg accgacctgc gagtgatgct
     3061 cgacgaactc agctacgtga agtcgacatc acgtcgaccc aagccgcgta acgacgagaa
     3121 tggtgcgccc aaggctggca atgggagcgg cgcaggcacg cggtcgcgct cgaccaaggt
     3181 catggtgcag ccggacacgg ttctgttcga tgcgccatcc attctggggg ctgccgaggt
     3241 ttcatctctg gcttccgctg ttgatggcgt agtgatgatc ctgcaccccg gcatcacgcg
     3301 tcgtggcgcg gcctccagag cggcagaaca gatcaggcgg gcaggaggca agatcgtcgg
     3361 catagtcatg gtcggtgtcc gagtcgacga cgactacagc gtgtacacgc cggtcgagga
     3421 ccccgatgac gatggtggac gcccgaccga ctcgccatgg tcgagggttg tttcgtccct
     3481 caagtcctaa cctgagcgga tattcggccc ccaggacgcg tattggccca gaatccgaaa
     3541 cggaacttcg ggaaagccgt tatctatggg tagcagccca tcgcgggatc gttccgcctg
     3601 gggttctggg gaggcacctt gaaggtccta tcagttgtaa gcagccttcc gtatcctccg
     3661 gatgacggga cgcgcagcac ggcctggaac ttcatccgag cgttgaactc gatcgcggat
     3721 gtgaccgtct gcacctgggt tacaggtaaa gaggatccgg atcacatcgc tcaagtgcga
     3781 tctgccgtca aggagttgat cacactggcc gatcgcggag gtcgcccaac gtggccggaa
     3841 cgcatcgagt ttcgggcgag atcgctgatg cttcgagtac ctccgtacat cctggagaag
     3901 agagatcgtt gtgatccgat gcctgaactc gatggattcg atctagctgt cgccgaagag
     3961 gatgccgcgc tgatgcttat gccgccgttt cgatgtccgg tagtggtaca ccgccacaac
     4021 atatttcccg acacgatcag gaccctcaga tcttcggggg ctctcggtac tgctcgcaaa
     4081 ctcaagtgga tctcggagat gccgatgtgg cgccgctaca accgggagtt gtcggagaag
     4141 gccacggtgt cgatcgccac cactcccgag gcggcggaga gtcttcgtcg ggtactgccg
     4201 ggagtggatg tattcgttgt ctgcaatggc gtcaagatac ctgaagaaca actgatccca
     4261 ggcccccaac cacggggcat cttcaccggg gtgatggact acgaacccaa cgcggacgcc
     4321 gccaccagct tcgttcgcga tctgtggcca cggatcgtct cggaagtgcc tgacgcctca
     4381 ttccagatca tcggacgcaa ccctctcaag aaagtgttgc gactctccgg ccagggagtc
     4441 gatgtcgtcg gtggggtacc tgaca
//
LOCUS       DATDOZ010000186         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_163649, whole genome shotgun sequence.
ACCESSION   DATDOZ010000186 DATDOZ010000000
VERSION     DATDOZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163649"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..2000
                     /gene="mrdA"
                     /locus_tag="VI541_03720"
     CDS             87..2000
                     /gene="mrdA"
                     /locus_tag="VI541_03720"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLF70046.1"
                     /translation="MTQGPDRTRLRVTFLGMTILSLISALVVRLWFLQILSGADLADA
                     AESNRVKYVPIEPPRGDILDRNGVVLVANRLSRAVAIQTEELPQDEHERTAVLRRLGE
                     VLGMSGEQIEGRLADKTVSPFSPIPVAEDVPKDSVIFIREHQDLFPGVLYQELPVRIY
                     PQGTLASHLLGYVGEINSDQLKEPYYSEYQAGSLIGRSGIEAAYERELHGRSGKVKLE
                     VNARGKVLGTLGRLDPQSGESLVTTIDSRIQKITEESLAQGIEKVRGVYDKDSGKRYL
                     APAGGAIVLDPNNGEIISMASYPGYDPSAFIGGISREGFAALADDPAHPLINRVTQAA
                     FPPGSTFKVITAAAALQDGVASSGARYNCPASYRFRDRTFRNWRSTDSGTMSVAQAII
                     DSCDTVFYPWGAEFYRRFLSGAGERLQDYARAFGLGAPSGIEIGDKAGRVPDEAWLKQ
                     QHRDHPRLFPNNVWLPGYTINMSIGQGDVLTTPLQMAGVYGSIANGGVLYKPHVGLRL
                     MRGDKTTRTMSSSVIRRLPVGVGAMATVRAGLQGVPTIGTARGAFAGFPLDRVPVAAK
                     TGTAELQTIPPQQPYAWFAAYAPAQAPSYVVVVMLEQGGHGGEAAAPIARRILEGLFN
                     LGLSDINPAARTD"
BASE COUNT          425 a          617 c          627 g          399 t
ORIGIN      
        1 cacccttctt acgccattcg tctacccaat cgttcgcaag atcgtcgcgt cctatcagcg
       61 cgacaaggtg tacaggtggt gaagcgatga cccaaggccc cgaccgcacg cgccttcgcg
      121 tgacattcct gggcatgacg atcctctcgc tgataagcgc attggtcgtg aggctgtggt
      181 tccttcagat actctcgggg gcagaccttg ctgatgccgc cgagtcgaat cgggtcaagt
      241 acgtaccgat cgagccaccc cgaggagaca tcctcgatcg caatggtgtg gtgctcgtcg
      301 ccaatcgact ttcgcgcgcg gttgcgatcc aaaccgagga actaccccaa gatgaacacg
      361 aacgaacagc cgtcctaagg cgcctggggg aagtgctcgg catgtcgggc gagcagatcg
      421 aaggccgact agccgacaag acggtttcac ccttctcacc gatccctgta gcggaggacg
      481 ttcccaagga ctcggtgatc ttcatccgcg aacaccagga tctcttccct ggggttctct
      541 atcaagagct tcccgttcgc atctatccgc agggcacgtt ggcatctcat ctgctcgggt
      601 acgtcgggga gatcaatagc gaccagctga aagagcccta ctacagtgaa taccaggctg
      661 gttcgttgat cgggcgcagc ggtatcgagg cggcatacga acgtgaactg catgggcgtt
      721 cgggcaaagt gaaactcgaa gtgaatgccc gaggcaaagt gctaggcacg ctcggaaggc
      781 ttgaccctca gagcggcgag tcactggtca ccacgatcga ttcgcgaata cagaagatca
      841 ccgaggagtc tctcgctcaa gggatcgaaa aggtgagggg cgtttacgac aaggactcgg
      901 gcaagagata tctcgctccc gccggaggag cgatagtcct agaccccaac aatggagaga
      961 tcatctccat ggcctcgtat ccgggttatg accccagtgc attcatcggc gggatctcgc
     1021 gcgaaggatt cgctgctctc gcggatgacc cggcacatcc cctgatcaac cgcgtgaccc
     1081 aggcagcctt cccacccggt tcgacattca aagtgatcac cgccgccgcg gcgctccagg
     1141 atggggtcgc atcttcaggt gcacgctaca actgtccggc ttcgtatcgg ttcagggacc
     1201 gaaccttccg gaactggcgc agcacagatt ccgggaccat gtctgtcgct caggcgatca
     1261 tcgactcatg cgatacggtc ttctatccat ggggagcaga gttttatcgc aggttccttt
     1321 cgggcgctgg agaacggcta caggactatg cgagggcctt cgggctgggg gcaccttcgg
     1381 gcatcgagat cggcgacaag gccggacgcg ttccagatga ggcctggctc aagcagcagc
     1441 acagggacca tccgcgcctg tttcccaaca atgtctggct accgggatac acgatcaaca
     1501 tgtccatcgg tcagggagac gtcctcacga ctccgctgca gatggcgggg gtttacgggt
     1561 cgatagctaa tgggggcgtc ctgtataagc cccatgtcgg tctgcgtcta atgagaggag
     1621 ataagacgac tcggaccatg tcgtcgagtg tcatccggag actgcccgtg ggggtgggcg
     1681 cgatggccac cgtcagagcc gggctgcagg gcgtgccgac cattggaacc gcgaggggag
     1741 ctttcgctgg cttccccctg gaccgagttc ccgtcgcagc caagacggga acggcggaac
     1801 tgcagacgat cccgcctcaa caaccgtacg catggttcgc cgcttatgct ccggcacagg
     1861 ctccttccta tgtcgtggta gtgatgctcg aacagggggg ccatggaggc gaggccgcag
     1921 cgcccatcgc ccgtaggatc ctcgagggtc tcttcaactt gggattgagc gacatcaacc
     1981 ccgcggcgag gactgactga tgattggcgc aactgtcttc tcaggctccc gcggtgatgc
     2041 gatgcatcgc ggttccggaa agtccccc
//
LOCUS       DATDOZ010000187         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_942513, whole genome shotgun sequence.
ACCESSION   DATDOZ010000187 DATDOZ010000000
VERSION     DATDOZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942513"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VI541_03725"
     CDS             <1..816
                     /locus_tag="VI541_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70047.1"
                     /translation="AYRGLGAWVDVFDYSLGNHMDVAAAVSEMSSRGVRTLYLETSRW
                     KEPQAFVDAATADLFIERAHAVGIKVVGWYLPGFGDIDRDIQRSLAVLQHSTPSGHRF
                     DGLAPDIENRQEVSQDRLAFNAGIIEYSKRLRAAVPAGTVLGAIVVDAKNNERAPERW
                     AGFPWPEIAKYYDIVMPMAYWSVTKKDCSVQYDAGAYINDVVAKTEALMGTSKPMHII
                     GGIANCTTTQEVEQYVAALRAVGSLGGGLYDFQTTQARPEKDQFWASLAGLNS"
     gene            complement(813..992)
                     /locus_tag="VI541_03730"
     CDS             complement(813..992)
                     /locus_tag="VI541_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70048.1"
                     /translation="MCLTCGCNLPHEDHGKSDYLTIEDLEKSAKLDNLDLDQAVQALI
                     KTVEVAKKEPDHAHS"
     gene            complement(1032..1217)
                     /locus_tag="VI541_03735"
     CDS             complement(1032..1217)
                     /locus_tag="VI541_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70049.1"
                     /translation="MITVVVIVGFFLVITGVLHFSIWAEKWLAVSEGSTLEFETDTLE
                     IAHAFFEPSEPEAIQAA"
     gene            complement(1423..1701)
                     /locus_tag="VI541_03740"
     CDS             complement(1423..1701)
                     /locus_tag="VI541_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70050.1"
                     /translation="MSIGASIFLAAVGAILRYAVTIPANQNGFNIHTGGVILMIAGGV
                     GLLLSMLFWNSNAPFNSRNSVSRREQTTTRDGVEQGTVVRETRDHVSS"
     gene            complement(1705..1908)
                     /locus_tag="VI541_03745"
     CDS             complement(1705..1908)
                     /locus_tag="VI541_03745"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLF70051.1"
                     /translation="MGSAKDKVTGRIKQSVGGLTGDEKLKREGRYDERVGRAKQKADE
                     TIDVVRDKLEGVTERLHPSEKEK"
     gene            complement(1923..2087)
                     /locus_tag="VI541_03750"
     CDS             complement(1923..2087)
                     /locus_tag="VI541_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010274703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein"
                     /protein_id="HLF70052.1"
                     /translation="MGPMLLVLLVALILFGLGFTMKILWWIALILIVVWLVGMIRPSS
                     GRRFYRWGNR"
BASE COUNT          437 a          709 c          674 g          485 t
ORIGIN      
        1 gcttaccgcg gcttgggggc ctgggtagat gtgttcgact actcactcgg caaccacatg
       61 gatgtagcag cagcggtatc ggagatgtcg agtcggggcg ttcgcacgct gtatctggag
      121 accagccgct ggaaggaacc gcaggctttc gtcgatgcag cgacagctga tctcttcatc
      181 gagcgtgccc atgctgtggg tatcaaggtt gtcgggtggt atctgccggg gttcggtgac
      241 atcgatcggg acatccaaag aagtctcgcg gtgcttcagc attcgacacc atccggacat
      301 agattcgacg ggctcgctcc cgacatcgag aatcgccagg aggtttcgca ggatcgcctc
      361 gcgttcaacg ccgggatcat cgagtactcc aagcgattgc gggccgcggt ccccgcgggg
      421 accgttctgg gcgccatcgt cgtggatgcg aagaacaacg agagagcccc cgagaggtgg
      481 gccggtttcc cctggccaga gatagccaag tactacgaca tcgtcatgcc gatggcctac
      541 tggtcagtca ccaagaagga ctgcagcgtc caatacgacg cgggggccta catcaacgac
      601 gtcgtcgcga agaccgaggc tctcatgggc acgagcaagc cgatgcacat catcggtggc
      661 atagcgaact gcacgaccac gcaggaggtc gagcagtatg tggccgcgct tagggccgtt
      721 ggatctctag gtggcgggct ctacgacttc cagacgaccc aagcccgacc cgagaaggac
      781 cagttctggg cgtcgcttgc cggactcaac tcttagctgt gggcgtgatc gggttccttc
      841 ttcgcgactt ctacggtctt gatgagagcc tgaacggcct gatccagatc caggttgtcg
      901 agcttggcgc tcttctcgag atcctcgatg gttaggtagt cggacttgcc gtggtcctcg
      961 tggggcaggt tacagccgca ggtgagacac atagatagaa cctcccgtta tcgctgaacc
     1021 ctgatagggg ctcaagctgc ttggatcgct tctggttcac tcggttcgaa gaaggcatgg
     1081 gcgatctcca gcgtgtcggt ttcgaactct agggtggatc cttcgctgac tgctagccac
     1141 ttttcggccc agatgctgaa atgcagaacc cccgtgatga cgaggaagaa gccgactatg
     1201 actaccactg taatcatgtg gcctccatcc gcaacgacgg gttatcggcc actctcgccg
     1261 cccagttccg cttcgcaata ttcacagcgc ggacgcggcc cccggcgcgg ccctccacag
     1321 tcttcgcaca ggatcgttcc aacgctggta gcaaggaggc cgagcccgag ggggctcggg
     1381 ctcggcgtct cgcttacgcc tatggaactg ggcgcattgg ggttagctag aaacatgatc
     1441 ccgcgtctcg cgaactactg tcccttgctc gacgccatct cgagtagtcg tctgttcacg
     1501 acggctgact gagttccgac tgttgaacgg cgcgttgctg ttccagaaga gcatggacag
     1561 caacagtcct acgccgcccg cgatcatcag gatgactcca ccggtatgga tgttgaaacc
     1621 gttctggttc gccgggatgg tgaccgcata acgcaggatc gcccccactg ctgctagaaa
     1681 gatacttgct ccgatactca tggtctactt ctccttttcg ctcgggtgga gcctctctgt
     1741 gactccctca agcttgtcgc gaacgacatc gatggtttcg tcggccttct gcttggctcg
     1801 gcctacgcgc tcgtcgtatc gcccttctcg cttcaacttc tcgtcaccgg tgagtccgcc
     1861 gaccgactgt ttgatccggc ctgtcacctt gtccttagca ctacccatcg atgcctccta
     1921 tctcaacgat tgccccaacg gtagaaacgg cggcccgagg agggtcggat catccctacg
     1981 agccacacca cgatcaggat cagcgcgatc caccaaagga tcttcatggt gaagccaaga
     2041 ccgaacagga tcagcgcgac cagcaatacc agcagcattg gacccatggc ttgcctcctc
     2101 tcccctacga gtgtgattcc cgcgtgtcac cgcgctgtca cctacgcgac tcggccgttc
     2161 tcctcgctcc cactcggccc ctcgaaatcc tcgacccact gcttgacggc ggcacagatg
     2221 tcatcggcgg aagtcgcgac ggcgaccggt tgttcggcgg ccatcgtgtc gagtgtctgg
     2281 gtgatgcgcg cgcgcagccc ggtct
//
LOCUS       DATDOZ010000188         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2004809, whole genome shotgun sequence.
ACCESSION   DATDOZ010000188 DATDOZ010000000
VERSION     DATDOZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004809"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VI541_03755"
     CDS             complement(<1..236)
                     /locus_tag="VI541_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70053.1"
                     /translation="MNIMRPGGLLGARDLLVHLTLRENRGKYKRSALGWAWSMVNPIA
                     TMLIFTLVFRVILKIDPPRGDPSGLKNFPFFLLSA"
     gene            255..1409
                     /locus_tag="VI541_03760"
     CDS             255..1409
                     /locus_tag="VI541_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF70054.1"
                     /translation="MSTPTTFALTEEQELFRKAVREFAESKIAPRAAEIDETDEFPWD
                     IHASLVEQGYMGVGFPEEFGGAGGGPLDVCILAEEISRVSAGVSLIHLVNRLGAIPVL
                     IAGTEAQKREASEGISSGKHQYSYCLTEPGSGSDAAAMASKAIRDGDGWRINGSKRFI
                     TNAGISDRYLYFAVTDPEGDKGKNVTAFLIHDDAEGFSLGKSERKMGIRGSPTREVNC
                     DNVWVPDSQVVGEVNQGFQIAMRTLDYSRPTIAAQALGIAQGALDESVRYVKEREQFG
                     QAIARFEGIQFMVADMAMAIEASRLLTYKAAALITEGDPDIGYFAAIAKAFASDTAMK
                     VTTDAVQLLGGYGYIKEFPVERMMRDAKITQIYEGTNQIQRVVIGRKILK"
     gene            1406..>2111
                     /locus_tag="VI541_03765"
     CDS             1406..>2111
                     /locus_tag="VI541_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70055.1"
                     /translation="MTFRLIYEIEPPRVPDLRKIHSQIEIFGPLIDAVLVPDNHLGRP
                     ALSSVAIALELKRAGVDPIVALNARDRNHLRFASDLMTMKAYGLNEVVFLYGDPIDSG
                     RTGLKVRQMLAHPEAQGIRRGVAANIGNKLGWRSEADFFMTQLAFGRGKPGYWREAHG
                     FSQPLYCGVIALRDREIARKIVGNIPGLQTPPGYLESFDHDPDAGFKMALTELSELRD
                     SGVDGAQLVVPTGRKRF"
BASE COUNT          446 a          621 c          653 g          391 t
ORIGIN      
        1 gcactgagaa ggaagaacgg aaagttcttg agcccactcg ggtcaccacg tggtgggtct
       61 atcttcaata tgaccctgaa gacgagggtg aagataagca tggtcgcgat cgggttgacc
      121 atcgaccacg cccagcccag cgccgacctc ttgtacttcc cgcgattctc tcggagcgtc
      181 aggtgcacaa ggagatcacg ggcccccagc agaccaccgg gcctcatgat gttcacgggc
      241 aatacaataa ccgtatgtcc acccccacaa cgttcgccct aacggaagaa caagagctct
      301 tccgcaaggc ggtccgcgag ttcgccgagt caaagatcgc gccgcgtgct gccgagatcg
      361 acgaaaccga tgagttcccc tgggatatcc acgcgagcct cgtcgagcag gggtacatgg
      421 gcgtcggatt ccccgaggag ttcggggggg ctggaggcgg cccactcgac gtctgcatcc
      481 tcgccgaaga gatctcccgc gtctcggcgg gagtcagtct tatccacttg gtcaaccggc
      541 tcggtgccat ccctgtcctc atcgccggaa ccgaagctca gaagagggaa gcgtctgagg
      601 ggatctcgtc cggtaagcac cagtactcat attgcctgac cgagcccggc tccggctccg
      661 atgcggccgc gatggcctcg aaggcgatcc gtgacggcga cggctggagg atcaacggct
      721 caaagcgttt catcacgaac gccggcatct cggacagata tctctacttc gccgttaccg
      781 atcccgaggg tgacaaaggt aagaacgtca cggctttctt gatccatgac gatgccgagg
      841 ggttctcgtt gggaaagtcc gagcgcaaga tgggtatccg tggatcgccc acacgcgagg
      901 tcaactgcga caacgtctgg gtccccgact cacaggtcgt tggcgaggtc aaccaggggt
      961 tccagatcgc gatgcggacg ctcgactact cgcgtccgac catcgccgcg caggctcttg
     1021 gcatcgccca gggggctctg gacgaatcgg tccggtacgt caaggagagg gagcagttcg
     1081 gtcaggccat agcgcggttc gagggtatcc aattcatggt ggcggacatg gcgatggcga
     1141 tcgaggcttc gagactcctc acctacaagg cggctgcatt gataaccgag ggcgaccctg
     1201 acatcggata cttcgccgcg atcgcgaaag cgttcgcatc ggacaccgcc atgaaggtaa
     1261 cgaccgatgc tgttcagctg ctcgggggat acggctacat caaggagttc cctgtcgaac
     1321 ggatgatgcg agatgcgaag atcacccaga tctacgaggg aacgaatcag atacagcgcg
     1381 tggtgatcgg tcggaagatc ctcaaatgac cttcaggctg atctacgaga tcgagcctcc
     1441 gcgggtgcca gaccttcgaa agatccactc tcagatagag atcttcggcc ccctgatcga
     1501 tgctgtgctc gtcccggaca accatctcgg tcggcctgcg ctctcgtcgg tggcgatagc
     1561 cctggaactg aagcgcgccg gggtcgaccc gatcgttgcc ttgaacgcgc gcgataggaa
     1621 ccacctgagg ttcgcgagcg acctcatgac gatgaaagcg tacggattga atgaggttgt
     1681 cttcctatac ggggacccca tcgactctgg gagaacgggg ctcaaggtcc gccagatgct
     1741 cgctcacccc gaagcacagg ggatacggcg gggggtcgcc gcgaacattg gaaacaagct
     1801 cgggtggcgg tctgaggcgg acttcttcat gacccagctc gcgttcggaa gaggcaagcc
     1861 tggctactgg cgggaagcac atggcttctc tcagccgctc tactgcggag tcatcgcgct
     1921 acgcgaccgt gagatcgcta gaaagatcgt tgggaatata ccggggcttc agaccccgcc
     1981 tggctatctg gagtctttcg atcacgaccc tgatgcggga ttcaagatgg cactcaccga
     2041 gttgagcgag cttcgggatt ctggcgtcga cggtgctcaa ctagtcgtgc cgaccggaag
     2101 gaagcgcttc g
//
LOCUS       DATDOZ010000189         3784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3856247, whole genome shotgun sequence.
ACCESSION   DATDOZ010000189 DATDOZ010000000
VERSION     DATDOZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3856247"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="carB"
                     /locus_tag="VI541_03770"
     CDS             complement(<1..1048)
                     /gene="carB"
                     /locus_tag="VI541_03770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HLF70056.1"
                     /translation="MPRRKDIGSVLIIGSGPIVIGQACEFDYSGTQACKVLKQEGLRV
                     ILVNSNPATIMTDPELVDRTYVEPITADIVEKVIARERPDACLPTLGGQTALNVAVQL
                     AEAGVFEAYGVELIGAKVEAIRVAEDRRLFREAMLGIGVDLPRSGFAYSIEEALEVAG
                     GIGLPVVVRASFTLGGAGSGFASSQEQLIDIVSEGLALSPISEVLIEESITGWKEYEL
                     EVMRDHKDNFVVVCSIENLDPMGVHTGDSITVAPAQTLTDVEYQQMRDVARDVIRRVG
                     VETGGSNIQFAVHPLTGRMVVIEMNPRVSRSSALASKATGFPIAKIAARLAIGYTLDE
                     IANDITSKTPASFEP"
     gene            complement(1174..2313)
                     /gene="carA"
                     /locus_tag="VI541_03775"
     CDS             complement(1174..2313)
                     /gene="carA"
                     /locus_tag="VI541_03775"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HLF70057.1"
                     /translation="MSRAVLVLEDGSTFEGESFGAPVDSLGEVVFNTGMTGYQEVLTD
                     PSYDGQIVNMTYPQIGNYGINDADVESSVPRVAGFVVRDAVAQWSSWRGKSSVDEYLS
                     EHGIGGISEVDTRRLTRHIRDRGAMRGGISSSLSAEELLAAVTNLPSMAGADLVGNVT
                     VKEPYVWPSEGKARFRVAAYDYGIKFNILRLLAAHGCETRVFPARTAAADVLADSPDG
                     IFLSNGPGDPEAVTYAVEAIDELLGAVPIFGICLGHQLLARAFGFDTYKLKFGHRGVN
                     HPVARLLDGAIEITTQNHGFAVSEAPFDYERPERPGLSLGRERIAQTPRGEVALTHIN
                     LNDYTIEGIRSLDLPAYSVQYHPEAGPGTHDSRYLFEGFVAMMQG"
     gene            complement(2310..3620)
                     /locus_tag="VI541_03780"
     CDS             complement(2310..3620)
                     /locus_tag="VI541_03780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLF70058.1"
                     /translation="MSSFILRGGRVIDPVSGTDVVADVVIEGSMVKEIGATPGASAEV
                     IDVSGAVVCPGFVDMHVHLREPGREDEETIASGSAAAALGGFTAICAMPNTDPVCDNA
                     AIAEKVAAQGRLIGLADVFPAGAITKGSEGLQLAPIGEMVRSRAAVRIFTDDGRGVQD
                     ARLLRRAMEYMRGFDVICAEHCEDSSLSEGGQMHEGEFSDLLGLKGIPAEAEEVALAR
                     DLALARMTGVRFHAMHVSTAGSVELIRRAKAQGVQVTAEVTPHHLSLTDAELVSYNPN
                     LKVNPPLRSASDVEALRRGVADGTIDVIATDHAPHAPEEKESEFESAPPGMLGLQTAL
                     GIMVGGLVGAGVVDLVALVALMSSRPAQILRIPGHGKLAIGEVANIVVFDPEAVWTVD
                     DADFASPVRNSPWIGKQMKGRVIHTIHDGDFTVRDGALMKGAFV"
     gene            complement(3617..>3784)
                     /locus_tag="VI541_03785"
     CDS             complement(3617..>3784)
                     /locus_tag="VI541_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HLF70059.1"
                     /translation="EALIMHPGPMNRGVEIGSDMADLERSIITDQVTNGIAVRMALLY
                     LMLGTDEEAVA"
BASE COUNT          726 a         1239 c         1086 g          733 t
ORIGIN      
        1 atggctcgaa cgaggcgggt gtcttcgagg tgatgtcatt agcgatctca tcgagggtgt
       61 agccgatcgc aagcctcgct gcgatcttag cgatcgggaa tcctgtcgcc ttcgaagcca
      121 gggccgatga tctcgaaacc cgtgggttca tctcgatcac gaccatccgt cccgtgagtg
      181 ggtgaacggc gaactggatg ttcgagccgc ccgtctcgac cccgacccgc ctgatcacat
      241 ctcttgcgac atcacgcatc tgctgatact cgacatcggt cagggtctgc gcgggagcca
      301 cggtgatcga gtcgcccgta tggacaccca tgggatccag attctcgatc gaacagacga
      361 ccacgaagtt gtccttgtga tcgcgcatga cctcgagctc gtactccttc cagcccgtga
      421 tcgactcctc gatgaggacc tctgatatcg gagagagagc caatccttca gagacgatgt
      481 cgatgagttg ctcttgcgag gaagcgaaac cagaacctgc gccgccgaga gtgaaggatg
      541 ctcgaaccac gacggggagc cctatgcccc cagcgacttc gagcgcctct tcgatcgagt
      601 atgcgaaacc tgacctgggc agatcaacac cgatcccgag catcgcctct ctgaaaagcc
      661 tgcggtcttc ggcgactcgg atggcctcaa ccttcgcgcc gatcagctca acgccgtagg
      721 cctcgaagac cccggcttcg gcaagttgca ccgcaacatt gagagcggtt tggccgccaa
      781 gggtcggcaa acacgcgtcc ggccgctccc tcgcgatgac cttctcgacg atatcagccg
      841 tgatcggctc gacataggtg cgatctacga gttccggatc cgtcatgatc gtcgccgggt
      901 tggagttcac aaggatcacg cgcaggccct cttgtttgag gaccttgcac gcctgcgtcc
      961 ctgaatagtc gaactcgcat gcctggccga tcacgatagg acccgacccg atgatcagga
     1021 cagatccgat gtccttgcgt cgcggcaagt cgcctatccc ttcctcttct tagccttcgc
     1081 caactctttg cgcttctcgg cctccttctt gcgctcctgt cgatcgcgct tccatctgac
     1141 cttcggtcgg tcacctctgc ccatcttccc ctcctatccc tgcatcatcg ctacgaaacc
     1201 ttcgaacaga tatctcgagt cgtgagtccc aggaccggcc tctgggtggt actgcaccga
     1261 gtacgcagga aggtccaggg aacggatgcc ttcgatcgtg tagtcgttga gattgatgtg
     1321 agtgagggcg acctcgcccc ttggggtctg cgcgatccgc tctctcccca ggctcaaccc
     1381 cggccgctcg ggccgctcgt agtcgaatgg ggcctcagag acggcgaatc catgattctg
     1441 agtagttatc tcgatggcgc catcgaggag ccttgctacc gggtggttca caccgcggtg
     1501 tccgaacttg agcttgtacg tatcaaagcc gaaggcacga gcgaggagct gatggccgag
     1561 acatatcccg aatatcggca cagcgcccaa cagttcgtct atcgcttcaa ccgcgtatgt
     1621 gacggcctct gggtcccccg gaccgttcga gaggaagatg ccgtcgggac tatcggcaag
     1681 cacgtcagcg gctgcggtgc gggcagggaa aaccctggtt tcgcagccat gtgccgcgag
     1741 gagtcgcagg atgttgaact tgatcccgta gtcatatgcc gcgacacgaa accgcgcctt
     1801 accctccgaa ggccagacgt atggttcttt caccgtcacg ttcccgacga gatcggctcc
     1861 ggccatcgag ggcaggttcg tcacggctgc gaggagttct tcagcggaga gcgacgagga
     1921 gatccccccg cgcatcgcac ccctgtcgcg gatatggcga gtcagccgac gcgtgtcgac
     1981 ctctgatatc ccaccgatcc catgctcgga gagatactca tcgaccgatg acttgcccct
     2041 ccacgagctc cactgcgcga cggcgtctct caccacgaaa ccagcgaccc gtgggacgct
     2101 actttcgaca tcggcatcgt tgatcccgta gttgccgatc tgggggtagg tcatgttgac
     2161 gatctggccg tcgtaagaag ggtccgtcag aacctcctgg tatccggtca tgcccgtgtt
     2221 gaatacgacc tctcccagcg agtcgacggg ggccccgaag ctctcgcctt cgaaggtcga
     2281 tccatcctcg aggaccaaca ccgcacggct catacgaacg cccccttcat gagtgcccca
     2341 tcgcgcacgg tgaagtcgcc gtcatggatg gtgtggatca cgcgaccctt catctgcttc
     2401 ccgatccatg ggctgttgcg aacaggggac gcgaagtcgg catcatcgac ggtccacacc
     2461 gcctcaggat cgaacacgac gatgttggca acctcaccta tggccagttt cccgtgcccg
     2521 gggatccgca agatctgtgc gggccgagat gacatgaggg cgaccagggc aacgaggtct
     2581 acgactccgg ccccaaccaa cccccccacc attatgccga gagcggtctg aaggccgagc
     2641 atccccggag gagccgactc gaactccgac tccttctcct cgggagcatg tggggcatgg
     2701 tccgtggcga tcacatcgat ggtcccatcg gcaacgccgc gacgcaatgc ctcgacatcc
     2761 gatgctgacc gcaggggcgg gttcaccttg aggttggggt tataactgac cagctccgca
     2821 tcggtgagtg acaggtgatg aggcgtgacc tcggccgtca cctggactcc ctgcgctttc
     2881 gctcttcgga tcaactccac agaaccggcg gtcgagacgt gcatcgcgtg gaacctcacg
     2941 ccggtcatcc gagcaagagc cagatcgcgg gcgagggcaa cctcttcggc ctctgccggg
     3001 atccccttca ggccgaggag gtcagagaac tccccctcat gcatctgacc gccctccgac
     3061 agagatgagt cttcgcaatg ctcggcgcag atgacgtcga aacccctcat gtactccatc
     3121 gctctgcgca ggagtcgcgc atcctgaact ccgcggccgt cgtctgtgaa gatgcggacg
     3181 gctgcgcgcg aacgaaccat ctcgccgatg ggtgccagct gcagaccctc cgaccccttc
     3241 gtgatggccc ccgctgggaa gacatctgcg agaccgatga gtctgccctg cgcagcgacc
     3301 ttctcggcga tagcggcgtt gtcgcagacg ggatctgtgt tgggcatcgc gcagatggcc
     3361 gtaaaaccgc cgagcgccgc tgccgcactt cccgacgcga tcgtctcttc gtcctcgcgc
     3421 cccggctctc tcaggtgcac atgcatatcg acgaatcccg gacagacgac cgctcccgag
     3481 acgtcgatca cctcggccga agcgccagga gttgccccga tctccttgac catcgagccc
     3541 tcgatcacaa catcggcgac cacgtcagtg cccgaaactg gatcgatcac tcggcccccg
     3601 cgaaggatga aagaactcac gcgacagcct cctcatcggt gccgagcatc aggtaaagga
     3661 gcgccatcct cacggcgatg ccgttggtta cctggtcggt gatgatcgag cgctcgaggt
     3721 cggccatgtc tgacccgatc tcgacaccgc gattcatcgg gccggggtgc atgatcagag
     3781 cctc
//
LOCUS       DATDOZ010000190         4235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2005847, whole genome shotgun sequence.
ACCESSION   DATDOZ010000190 DATDOZ010000000
VERSION     DATDOZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005847"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VI541_03790"
     CDS             <1..1229
                     /locus_tag="VI541_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HLF70060.1"
                     /translation="NAEYLARQEMAARGWDNDAYLLRTMPEDDRSAYESEYEPILEKK
                     RTECQEEHRTVVDLGGLYVLGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLM
                     RFFATGMINTVMERFNIPEDLPIEAKLVSKAIERAQNQVESQNFEIRKNVLKYDEVLN
                     RQREEVYRARRRLLDGEDLSERSLNMVADVIEATINNFAAPGVHPDEWDWDGLEPALA
                     SLYPTKLEGKFDRQTTEHDELLEAYMEEAVEFYRSREAEIGSEKFRDLERFVLLSVLN
                     AKWREHLYEMDHLREGIGLRAYGQRDPLVEYQREAFDMFAVLMATLKEEFVRYMFHVD
                     VIAQEQTASQDQTRRINSPVSTASAMSAARQAAGEAQMEAGLSREAARSEKIPRNAPC
                     PCGSGKKYKMCHGANE"
     gene            1274..2342
                     /gene="prfB"
                     /locus_tag="VI541_03795"
     CDS             join(1274..1285,1287..2342)
                     /gene="prfB"
                     /locus_tag="VI541_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141201661.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLF70061.1"
                     /translation="MADVRSSFDVERKRARAQELRDLASQPGFWDEQAQATRKMGELS
                     RLEDDLKVFASISEQLEEASVMLQLSQEESDAGALAEAEAAATDVSQRLDDLEVRTLL
                     SGDYDESDAIASIHAGEGGTESCDWAAMLLRMFLRWAERKGLVAEILDSQPGDEAGIK
                     QVTFTLKGRFAYGLLSTEKGVHRLVRISPFDASKRRHTSFASLDVVPVIEDEGEVPID
                     AGDLRIDIFRSGGPGGQSVNTTDSAVRITHLPTGLVVSVQNERSQLQNKALAMRILAS
                     RLADRLRAERQQELEELRGEQLEIGFGSQIRSYVLHPYQMVKDRRTEVETGNVDAVLD
                     GDIDRFIAAELRRRKKGKPQN"
     gene            complement(2348..3286)
                     /locus_tag="VI541_03800"
     CDS             complement(2348..3286)
                     /locus_tag="VI541_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLF70062.1"
                     /translation="MTRELGAPTRDAFGEALAELGAQNSRIVVVDADVTESTRTHTFM
                     ERFPERSWNTGIAESNLVGIGAGLAASGKIPVIASFACFLTCNAYDQIRMSIAFPNLN
                     AKLVGSHGGISPGEDGPSQMAVEDVALMGALAGFTVIVPADASSTKALTRAMIEHVGP
                     TYMRTGRPKAPVIYESEPKVTLGRAIEIRAGSDVTIIANGLMVAEALDAADELATQGL
                     SARVLDMHTVKPIDAEAIVSAARDTKGIVVAEEHFAHGGLGSAVAMVVGAQAPTRMAF
                     VNIGDTYSTSGQPDELFEAAGITSSDIGKAALSLMS"
     gene            complement(3283..4122)
                     /locus_tag="VI541_03805"
     CDS             complement(3283..4122)
                     /locus_tag="VI541_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLF70063.1"
                     /translation="MCLSEVWMTELEEIAKRVRRHIVETTTAANSGHPSSSLSAVEIG
                     TVLFFGGVLRYDAKNPTWEDRDRFILSKGHAAPLLYALLAESGFIDISLLPTLRKFGS
                     ILEGHPNLRRVPGVEASTGSLGQGLSQGIGQALAAKLDGRDSRVYVLQGDGETQEGQV
                     WEAIMFAANHNIDNLTLIIDANTYQQSGSVASIQPLDPLADKLRAFRWDVTEIDGHSM
                     DQVVKAFEWAKGSIGVPQAIIAHTVKGRGVAFLEEQGNWHGKPVPADRLEEALADIER
                     GLD"
BASE COUNT          863 a         1244 c         1313 g          815 t
ORIGIN      
        1 gcaacgctga gtatctggct cggcaggaga tggccgctcg gggttgggac aacgacgcgt
       61 acctcctgcg caccatgccc gaagatgacc gttctgcgta cgaatctgag tacgaaccga
      121 tcctcgaaaa gaagaggaca gagtgccagg aagagcaccg cacggtcgtc gatctcggtg
      181 ggctctacgt tcttggcaca gaacggcacg agtcgcggcg tatcgataat cagttgcgag
      241 gtcggtcggg ccgtcagggt gaccccggcg agagtcgctt ctatctctcc ctcgaagacg
      301 atctgatgcg cttcttcgcg acggggatga tcaacacagt catggagcgc ttcaacatcc
      361 ccgaggacct cccgatcgag gcgaaactgg tttcgaaggc tatcgagagg gcccagaacc
      421 aggtcgagtc gcagaacttc gagatccgca agaacgttct gaagtacgac gaggtcctga
      481 accgccagcg agaagaggtc taccgcgccc ggcgccgatt gctcgacggc gaagatctat
      541 cagagcgttc actcaacatg gtcgccgacg tgatcgaggc aaccatcaac aacttcgccg
      601 cccccggggt gcaccctgat gagtgggatt gggatggtct tgaaccagcg ttggcctcgc
      661 tctatcccac gaagttggaa ggaaagttcg accgccagac gacagagcac gatgaactgc
      721 tcgaggcata catggaggag gcggtggagt tctaccgcag tcgcgaagcc gagatcggct
      781 cagagaagtt ccgtgacctg gagcggttcg tccttctctc ggtcctcaac gccaagtggc
      841 gagagcacct ctatgagatg gaccaccttc gtgaagggat cgggcttcgc gcttacgggc
      901 agagggatcc gctcgttgag taccagcgcg aggccttcga catgttcgcg gtcttgatgg
      961 ctacgctcaa agaggagttc gtccgttaca tgttccatgt cgatgtcatc gcgcaggagc
     1021 agacagctag ccaggaccag acacggcgga tcaacagccc tgtgagcacc gcctctgcga
     1081 tgtctgcggc ccgtcaggcc gcgggcgaag cacagatgga ggcaggactc tcacgggagg
     1141 ccgcgaggtc agagaagatc ccgcgcaacg cgccatgccc atgcggttca ggtaagaaat
     1201 acaagatgtg ccatggggcc aacgaatgat gatcgaagat ctccccggat tcataggggg
     1261 gcttgtagag aagctggccg acgttaagga gttcctttga cgtagagcgc aaacgcgcta
     1321 gggctcagga gcttcgcgac ctggcttcgc agcccggatt ctgggacgag caggcgcagg
     1381 caactcgcaa gatgggcgaa ctctctcgtc tcgaggatga cctgaaggtc ttcgcctcga
     1441 tatcagaaca actcgaagaa gcctcggtga tgctccagct ctcgcaggag gagtccgatg
     1501 cgggcgcact cgccgaggcc gaggccgccg ccactgatgt ctcccaacgg ctggacgatc
     1561 tcgaggtgag gacccttctt tccggcgact atgacgaatc cgacgccatc gcgagcatcc
     1621 acgcgggcga gggtggcacc gaatcctgcg actgggccgc gatgctcctg cggatgttcc
     1681 ttcgatgggc agaacgcaaa gggctcgtgg ccgagatcct cgactcgcaa cccggtgacg
     1741 aagcgggcat caaacaggtc accttcacgc tgaaaggtag gttcgcgtac gggctgctct
     1801 cgacggagaa aggtgttcat cgtctggtgc gcatctcgcc gttcgatgcg tcgaagcggc
     1861 gtcacacatc gttcgccagt ctcgatgtcg tgccggtgat cgaggatgaa ggagaggtcc
     1921 ccatcgatgc gggagacctt cgcatcgaca tattccgctc aggggggcct gggggccagt
     1981 cggtaaacac caccgactcg gccgtgcgca tcacgcatct tccaactggc ctggtggtct
     2041 cagtccagaa cgagaggagt caactccaga acaaagccct tgccatgcgg atcctcgcat
     2101 ctcgcctcgc cgacaggttg cgtgcggagc ggcagcagga actcgaggaa ctgcggggag
     2161 agcagttaga gatcggcttc ggctcccaga tccgttcgta cgttctgcat ccctatcaga
     2221 tggtgaagga tcgacggacg gaggttgaga caggcaatgt cgacgcggtg ctagatggag
     2281 acatcgatcg gttcatcgcc gcagaattgc gacgccgcaa gaagggcaaa ccccaaaact
     2341 gagtccctca ggacatgagc gagagcgcgg ccttgccgat gtctgatgac gtgatcccgg
     2401 ccgcttcgaa cagctcgtct ggttggcccg atgtcgagta ggtgtcgccg atgttcacga
     2461 atgccatccg cgtaggagcc tgggccccca cgaccatagc gacagcggag ccaaggcccc
     2521 catgcgcgaa gtgttcttcg gcaacgacga tgcccttggt atcacgagcg gctgagacga
     2581 tggcctcggc atcgataggt ttgaccgtgt gcatatcgag gacgcgcgca gagagaccct
     2641 gggtcgcaag ctcgtcagcg gcatcgagag cttcggccac catgagaccg ttcgcgatga
     2701 tggttacgtc actgccagcg cggatctcga tggcccttcc gagagtgacc ttgggctcgg
     2761 actcgtagat caccggtgcc ttcggcctgc cggtccgcat gtatgttggt ccgacatgct
     2821 cgatcatggc gcgagtgagt gccttggtcg acgacgcgtc cgctggaacg atcacggtga
     2881 atcccgcgag ggctcccatg agggcaacat cttcgacggc catctgcgaa ggtccgtcct
     2941 cacccggtga gatgcccccg tggctgccga ccaacttcgc attcaggttc ggaaaggcga
     3001 tgctcatgcg gatctgatcg tatgcgttgc aggtgaggaa gcaggcgaag ctcgcgatca
     3061 cagggatctt gccgctcgcc gccaaccctg cgccgatccc gacgagattc gactcagcga
     3121 tccccgtgtt ccatgaacgc tcgggaaagc gctccatgaa agtgtgcgtg cgtgtcgact
     3181 ccgtgacgtc ggcatcgacg acgacaatgc gagagttttg tgcgcctagc tcggccagag
     3241 cctcgccgaa tgcgtcgcgt gtgggggccc cgagttcgcg agtcaatcca atcccctttc
     3301 gatgtcggcg agggcttctt cgagtctgtc tgcaggtact ggcttgccgt gccagttgcc
     3361 ctgctcttca aggaaagcaa cgccacgccc cttgaccgtg tgggcgatga tcgcctgcgg
     3421 cacgccgatc gacccctttg cccactcgaa ggctttgaca acctgatcca tcgagtgccc
     3481 atcgatctcg gtgacatccc agcggaacgc acgaagcttg tcagcgagag ggtcgagggg
     3541 ctggatgctc gcaaccgaac cggactgctg atatgtgttc gcatcgatga tcaatgtgag
     3601 gttgtcgatg ttgtggttgg cggcgaacat gatcgcttcc cacacctgcc cctcctgtgt
     3661 ttcaccgtcg ccctgcagga cgtacacgcg cgagtcgcga ccgtctaact tcgcagcaag
     3721 cgcctgacct atgccttgcg agagtccctg tccgagcgaa cctgtcgaag cttcgacacc
     3781 cggtacgcgg cgcaggttcg gatgtccttc gaggatcgag ccgaacttac gcaatgttgg
     3841 aagaagtgag atgtcgatga accccgactc cgccagcagc gcatacagca gaggagcggc
     3901 atgtcctttc gagaggatga agcggtctcg atcctcccat gtggggttct tcgcgtcgta
     3961 acgaaggacc ccaccgaaga acagaacggt tccgatctcg accgcagaga gcgaactgct
     4021 agggtggcct gagttcgcag cggtcgtggt ttccactatg tgacgtctga cgcgtttcgc
     4081 tatctcttca agttctgtca tccacacctc gctcaggcac agtggtcgtg tgacgcccgt
     4141 attcttatca tctttcgcgg gcgcaagttc cccgtcgaga actgggggcc gtgctagcat
     4201 cttgtgcttg aatcatcctt agggttcgac actgc
//
LOCUS       DATDOZ010000191         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_164652, whole genome shotgun sequence.
ACCESSION   DATDOZ010000191 DATDOZ010000000
VERSION     DATDOZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164652"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2282
                     /gene="acnA"
                     /locus_tag="VI541_03810"
     CDS             <1..2282
                     /gene="acnA"
                     /locus_tag="VI541_03810"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HLF70064.1"
                     /translation="PFVPVDLIIDHSVQVDRFGSEAAYGANIEWEYHRNAERYSLLRW
                     AQQAFEGFRVVPPGMGICHQVNLEYLGQVVSNKDGLAFPDTLVGTDSHTTMINGLGIL
                     GWGVGGIEAEAAMLGQPMFLTTPVVLGIRTRGALPTGTTATDLVLTLTNMLRAHGVVG
                     KFVEFFGAGLSSLSIADRATLSNMSPEFGATATLFPVDVNTLRYLTDTGRGAVVPLVE
                     TYTKEQGLFRTDEDPEPRFSEVLDLDLSTVEPSLAGPRRPQDRVPLRDVRVSFDAAFP
                     ERSTTSDEASVRDGSVVIAAITSCTNTSNPSVMIGAGLLARNAVEAGLQTKPWVKTSL
                     APGSRVVTRYLEKAGLMPYLNKLGFNLVGYGCTTCIGNSGPLPDDIAAMVDEHQLSVV
                     AVLSGNRNFEGRIHPQVKASYLASPPLCVAFALAGTIDIDMTTQPLGVNSEGNEVFLS
                     DIWPSGTDIDELLKTSISKDQFDDEYGRIFEGDDDWKQMPSPTGEMFAWDPASTYVQE
                     PPFFESTTTDFEAPGDVEGARALVLLGDSVTTDHISPAGSIKLHSPAGEYLQGLGVQT
                     QDFNSFGSRRGNHQVMLRGTFGNIRLRNQIAPGTEGPWTTHLPSGETMSVYDASLRYK
                     QEGVPLVAIAGKEYGSGSSRDWAAKGTALLGIRAVLAESYERIHRSNLVGMGVLPLQF
                     RKGESAQTLGITGTERFTITGVSGEIKPGQDAQVVAESDDGKKTIFTATVRIDAPAEV
                     EYYRNGGILTTVLRELLKG"
     gene            2344..3021
                     /locus_tag="VI541_03815"
     CDS             2344..3021
                     /locus_tag="VI541_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLF70065.1"
                     /translation="MGTGAAIQAATGNGFGLISAPFLVAAVGATMGIPAALALSMVVN
                     ILVFVRDPSGVRWRTGALLLVPALIVIPFTARLVRSTGPQLLAILAGGLTLAAVIALA
                     SGRRSRRLGRPAAAVAAGMLSGAMNVIGGISGPPTVIYTINAGWPNRDIRSTLQIYFF
                     ILNVAALVSLGVRPQIDWRWIVAGVAGWLIGVITLKKVAEARLRILMLLVAAVGGSVA
                     LYHGLTG"
     gene            complement(3003..>3286)
                     /locus_tag="VI541_03820"
     CDS             complement(3003..>3286)
                     /locus_tag="VI541_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLF70066.1"
                     /translation="RAPNLPDAVLASEVLGPPDLEERFGLTGGNIFHGEITPDQSFGE
                     RFPYRTEIAGLYLCGSGASPGGGVMGAAGRNAARCVIADMRSAGQPVNP"
BASE COUNT          612 a         1054 c          990 g          630 t
ORIGIN      
        1 aacccttcgt gccggtcgac ctcatcatcg accactcggt ccaggtcgat aggttcggct
       61 cggaagcggc ctacggggcc aacatcgagt gggagtatca ccgcaatgca gagcgctact
      121 ccctccttcg ctgggctcaa caggctttcg aggggttccg tgtggtcccg cccggcatgg
      181 ggatctgcca ccaggtgaac ctggagtatc tgggccaggt cgtctcgaac aaggatggcc
      241 tcgccttccc cgacactctg gttgggaccg actctcacac cacgatgatc aacggcctgg
      301 gcatcctcgg atggggtgtc ggcggtatcg aggccgaggc cgcgatgctg ggccagccca
      361 tgttcctcac cacgccagtc gtactcggca tccgcacaag gggcgcgctt ccgacaggga
      421 ctaccgcgac cgatctcgtt ctgacactca ccaacatgct tcgcgcccat ggcgtcgtcg
      481 gcaagttcgt cgagttcttc ggggccgggc tatcgagtct ctcgatcgcc gaccgcgcca
      541 cactttcgaa catgtccccc gagttcggcg caaccgctac cctcttcccc gtcgacgtca
      601 acacacttcg gtacctcacc gacaccggca ggggtgccgt cgttcccttg gtcgagacat
      661 acacgaagga gcagggactc ttccgcaccg atgaggaccc cgaacctcgg ttctctgagg
      721 ttctcgacct cgacctatcg accgtcgagc cgagtcttgc cggtccacga cgaccgcaag
      781 accgcgtgcc tctgagggat gttcgagttt ccttcgacgc ggcattcccg gagcgatcga
      841 caacttcgga tgaggcttcg gttagagatg gttcggtcgt catcgccgcg atcacatcgt
      901 gcaccaacac ctcgaacccc tcggtgatga tcggagcagg gcttcttgca cgaaatgcgg
      961 tcgaagctgg tctgcagacg aagccatggg tgaagacttc acttgcgccc ggctcacgcg
     1021 tcgtgacccg ttacctcgag aaggcgggcc tgatgccata cttgaacaaa ctcgggttca
     1081 acctggtcgg gtatggctgt accacctgca tcggtaactc gggccccctc cccgacgaca
     1141 tcgccgcgat ggtcgacgag catcagttgt ctgtggtcgc cgtcctatcg ggcaatcgca
     1201 acttcgaagg ccggatccat ccgcaggtca aggcgagcta cctcgcatcc cctccgctct
     1261 gcgttgcatt cgccctcgcg ggcacgatcg acatcgatat gaccacgcag ccattgggag
     1321 tgaactccga aggtaacgaa gtcttcctct ccgacatctg gccatcagga actgacatcg
     1381 atgaactact gaagacatcg atctcgaagg atcaattcga cgatgagtat ggccgtatct
     1441 tcgagggaga cgacgactgg aaacagatgc cgtcaccgac tggcgagatg ttcgcatggg
     1501 accccgcatc cacctacgtg caggagccgc cattcttcga gtcgaccacc accgacttcg
     1561 aggcccccgg tgacgtcgaa ggagcgcggg ctcttgtcct gcttggcgac tcggtgacga
     1621 ccgaccacat ctcgccagcg gggtcgatca agctgcactc ccccgcgggc gaatatcttc
     1681 aagggctcgg cgtgcagact caggacttca acagtttcgg ttcgcgtcgc ggcaaccacc
     1741 aggtgatgct gcgcggcaca ttcggaaaca taaggttgcg caaccagatc gcacccggaa
     1801 cagagggccc ttggaccact cacctgccga gcggagagac gatgagcgtc tacgacgcat
     1861 ctctacgata caagcaggaa ggcgtccctt tggtagctat cgcaggcaag gagtatggct
     1921 ccggtagttc gcgagattgg gcggcaaaag gaacggcact gcttggtatc cgggccgtgc
     1981 tcgccgagag ttacgagcgt atccatcgaa gcaacctcgt ggggatgggg gtccttcctc
     2041 tgcagttccg taagggtgag tcggcccaga cactcgggat cacggggacc gagaggttca
     2101 cgatcactgg cgtatcggga gagatcaagc cgggccagga tgcccaggtc gtcgccgaga
     2161 gcgacgacgg taagaaaacg atattcaccg cgactgttcg aatcgatgct ccggcagagg
     2221 ttgagtacta ccgcaacggc ggcatcctca cgacggtcct tcgtgagttg ctgaagggat
     2281 gaccctcaac gcatcggtag gggacgctct ctgatcacga gcggcctgct cgctctattc
     2341 gccataggaa cgggtgcggc gatccaggcg gcgacaggca acggattcgg cctcatctca
     2401 gcaccgttcc tggtagccgc tgtgggtgca accatgggca tccccgccgc gttggcgctt
     2461 tcgatggtcg tgaacatcct cgtcttcgtt cgagaccctt ctggcgtgcg ttggcgaacg
     2521 ggggctcttc tcctcgtacc agcgctgatc gtgatcccct tcacagcccg attggttcgc
     2581 tcgaccggcc cccaactact cgcgatcctc gctggcggac tgaccttggc cgcggtgatc
     2641 gccctcgcta gcggacgtcg gtcacgtcgt ctcgggcgcc ccgcggccgc ggtcgccgct
     2701 gggatgctga gcggtgcgat gaatgtcatc ggtggcatca gcgggccacc gaccgtcatc
     2761 tacacgatca acgctggctg gcccaatcgc gacatccgct cgaccctgca gatctacttc
     2821 ttcatcctca acgtcgcggc tctcgtgagc ctcggtgtca gaccccagat cgactggcga
     2881 tggatcgtgg caggtgtcgc ggggtggctc atcggggtca tcactctgaa gaaggtcgca
     2941 gaggcccgac ttcgcatcct gatgctgctg gtcgccgccg ttggcggctc agtcgctctc
     3001 tatcacgggt tgacaggttg accggctgat cgcatgtcag cgatcacgca gcgagccgcg
     3061 ttgcgtccag cagcccccat cactccgccc ccaggagacg caccggaccc gcacagatac
     3121 agcccggcta tctcggtgcg atacgggaag cgctcaccga aggattgatc gggagtgatc
     3181 tcgccgtgga agatgttccc gcctgtcagc ccgaaacgct cttcgaggtc gggggggccg
     3241 agaacctctg atgcgaggac cgcgtccggc aggttggggg cccggc
//
LOCUS       DATDOZ010000192         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3330379, whole genome shotgun sequence.
ACCESSION   DATDOZ010000192 DATDOZ010000000
VERSION     DATDOZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330379"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VI541_03825"
     CDS             complement(<1..180)
                     /locus_tag="VI541_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF70067.1"
                     /translation="MTEMILRCKGLVKHFGDRTAVDDVGFEIAAGESYGLLGPNGAGK
                     TTTISMICGLLRRDAG"
     gene            complement(177..776)
                     /locus_tag="VI541_03830"
     CDS             complement(177..776)
                     /locus_tag="VI541_03830"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLF70068.1"
                     /translation="MRPLPTTSYAVLGLLSIQPMSGYDVTRFAEQSVAHFWPIVKSQV
                     YSELNRLEELGFISGTDVAQEGVPDKRVFQPTPGGLAALDAWLIAPGYAAGRVRSGFL
                     VKFFFGHRLDTVRLIELVKEFRAESQADLDRFGPLIEVGKKHPAMTFPMASATFGMHH
                     LEANIRWADGVLADLQKRRANESTTKTSRSKAPKKGGKG"
     gene            881..1909
                     /locus_tag="VI541_03835"
     CDS             881..1909
                     /locus_tag="VI541_03835"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLF70069.1"
                     /translation="MRIPERPRVAVVGATGAVGRVLLDLLIERNFPASEVVAVASQRS
                     EGRVLPFGDGDLTVTALSEGVFEGIDIAIMDTPDEVALEWAPIAVRAGAVVVDNSGAW
                     RMDEKVPLVVPEINPGALLTHEGIIASPNCTTIGVVVPLWALHRAFGVERVVVSSYQA
                     TSGAGQPGVEELREQAEKLHGEFDALKVGAIEGLAPPPRMFADTIAFNVVPQIGSIAQ
                     QGYTGEEWKLLYESRKIMGLPDLDMTGTCVRVPTVAGHGSSVLAWFRDEVDVASATEA
                     LRAAPGVALADVPTPLLAAGKDACFVGRVRRHPSDAHALWFFTTSDNLRKGAALNALQ
                     IAESLLGR"
     gene            1928..2004
                     /locus_tag="VI541_03840"
     tRNA            1928..2004
                     /locus_tag="VI541_03840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1962..1964,aa:Pro,seq:cgg)
BASE COUNT          373 a          634 c          692 g          426 t
ORIGIN      
        1 gcccgcgtct cgccgaagaa ggccgcagat catcgagatg gtagttgtct tgcccgcacc
       61 gttgggaccg agaagaccgt aggattcgcc agccgcgatc tcgaagccga cgtcgtcgac
      121 ggcggtccgg tcgccgaagt gtttcaccag ccccttgcag cgcaggatca tctcggtcat
      181 cccttgcctc ctttcttagg ggctttggat ctggacgttt tcgtcgtcga ctcgtttgcg
      241 cgacgcttct gaaggtcggc cagcactccg tcggcccagc ggatgttggc ttcgaggtgg
      301 tgcatgccga acgtcgcgct tgccatcggg aaggtcatcg ccgggtgctt cttgccgacc
      361 tctatcaggg ggccgaatcg gtcgaggtcg gcctgggact cggctctgaa ctccttgacc
      421 aactcgatca accgcacagt gtcgagtctg tggccgaaga agaacttcac cagaaagccg
      481 ctgcgcactc tccctgccgc atacccgggt gctatcagcc aggcatcgag tgctgcaagg
      541 ccccctggtg tcggctggaa tacgcgtttg tcgggaacgc cctcttgcgc aacgtcggtt
      601 cccgagatga agccgagttc ctcgagacgg ttgagttccg agtagacctg actcttcacg
      661 atcggccaga agtgagcgac cgactgctcg gcgaaccgcg tgacgtcgta tcccgacatc
      721 ggctggatgc tcagtaggcc gaggacggcg tagcttgtgg ttggcagggg tcgcatcggc
      781 ggtgactgta gcagggctac taggactagg tcaagtagaa ctagaccatt tcgtcctagt
      841 tgtccgatga ctcaaggccc gtcgtcgcta tattggcttc gtgcggatcc ctgagaggcc
      901 ccgagtcgcc gttgtcggtg ccaccggcgc agtgggtcgc gtcctccttg acctactcat
      961 agagcgaaac tttccagcca gcgaggtcgt ggctgtcgct tcgcagcgct ctgagggacg
     1021 ggtactaccg ttcggtgatg gtgacctgac ggtgacagca ctatcggaag gagttttcga
     1081 ggggatcgat atcgccatca tggacactcc cgacgaagtc gcgcttgagt gggccccgat
     1141 cgcggtaagg gcgggcgctg tcgtcgtcga caactcgggg gcctggcgta tggatgagaa
     1201 ggtcccactc gtggtgcccg agatcaatcc cggggcccta cttacgcatg aagggatcat
     1261 cgcctctccc aactgcacca cgatcggagt ggttgtcccc ttgtgggcgc tgcaccgcgc
     1321 cttcggtgtg gagcgcgtcg tcgtgtcgtc gtatcaagcg acatcggggg ccggtcagcc
     1381 gggcgtagag gagctgcggg agcaggccga gaagctccat ggcgagttcg acgccctcaa
     1441 ggtcggagcc atcgaaggac tagcgccccc gccgcgcatg ttcgccgaca cgatcgcatt
     1501 caacgtggtt ccgcagatcg ggtctatcgc gcagcagggg tataccggcg aggagtggaa
     1561 gctcctatat gaatcgcgaa agatcatggg gctccccgat ctcgacatga ccgggacatg
     1621 tgttcgcgta ccgacggtgg cggggcatgg gtcgtcggtg ctggcatggt ttcgcgatga
     1681 ggtcgatgtc gcgtcggcca ccgaggcgct gagagcagct cccggagtcg cactggctga
     1741 tgttcccacc cccctgctcg ccgcgggtaa ggacgcgtgc ttcgtgggcc gtgtccgtcg
     1801 tcacccatcc gatgctcacg ccctatggtt cttcacaacg tcagacaatc tgcgaaaggg
     1861 tgcggccctg aacgcgctcc agatcgcgga gtctctactg ggtcgttagg ggtacactga
     1921 cccgactcgg gatgtagcgc agcttggtag cgcgcctcgt tcgggacgag gaggtcgtgg
     1981 gttcaaatcc cgccatcccg accagttcgt caaggcacct tagcaagatg acccaggagg
     2041 caccgggttg gagttagaca aggtcgtcgc gcgacgccgc atggtccgga actacaccgg
     2101 cgagtccatc gagcaggaga gtctc
//
LOCUS       DATDOZ010000193         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_165101, whole genome shotgun sequence.
ACCESSION   DATDOZ010000193 DATDOZ010000000
VERSION     DATDOZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165101"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VI541_03845"
     CDS             complement(<1..624)
                     /locus_tag="VI541_03845"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131579.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HLF70070.1"
                     /translation="MLIVMRKDSTTDEVESVLSKVRATGTECFVSRGDFHTVIGIVGD
                     PEPLEELPLAAMRGVERVQRVTKSYKVVSHEGHPGSSTVAVGSASIGPDTFTIIAGPC
                     AVENEEQTIAAAMAAKKSGAHILRGGAYKPRTSPYSFQGLAEDGLKILETARQETGLP
                     IVTEVMDARDVDKVAAVADMLQVGTRNMQNFPLLKEIGSTGKPVMLKR"
     gene            684..1262
                     /locus_tag="VI541_03850"
     CDS             684..1262
                     /locus_tag="VI541_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70071.1"
                     /translation="MSYTRRAVHWDADTRGRRDFERQVRQTIKDDGELVYLDSTDSFD
                     EIDFELGCRGRRLFVEVKEKLQHYRAAWQEASAVPEEDLFILDELAARKIILRAPRAY
                     LLIHDAICERLSVFGALELLTAPKDRLDRSIEANVATFKGKWLIDLLHGFECASLAEA
                     LDYIKRRSTSEEDAWQGLACHGRYRDEHLPRI"
     gene            1346..2440
                     /gene="moeB"
                     /locus_tag="VI541_03855"
     CDS             1346..2440
                     /gene="moeB"
                     /locus_tag="VI541_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2246..2248,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HLF70072.1"
                     /translation="MLAEARRKAAALEPEQVDEIRRNEDVLLVDVRESDEWRAGHLPG
                     AIHLPRGFFELQIDEKLPDKTKPVILYCAGGLRSALAAMTMEQLGYTDVRHMNGGFTR
                     WRDGGFEVVADRQWTPEQRDRYSRHFILPEVGEAGQQKISSGSVLVVGAGGLGSPALY
                     YLAAAGVGRLGIVDFDAVERNNLQRQIIHSTARIGTNKAESAAATIVDLNPDVKVETW
                     TERITPDNVDSIVDGFDVIIDATDNFVTRYMLNDAAVRFAKPYIYGSIFRFEGYASVF
                     WPAEGGPCYRCLHPEAPPEHLAPTUGEAGVLGVLPGIVGSIQANEALKILVGYGEPLV
                     GRLLTFDAHDTRFGELKLRRLADCPTCGGK"
     gene            complement(2483..2836)
                     /locus_tag="VI541_03860"
     CDS             complement(2483..2836)
                     /locus_tag="VI541_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70073.1"
                     /translation="MSSSTTAWEGRFGDADLKIEIPPSGRIHAEWKTQAGTRQKVVDS
                     IEQLERSVLFQLMVGTETSDLAREEVSDAVALSLANRQPFPSQDPSQSARPVRKRPKP
                     GQQRRRGSYRPQRKR"
     gene            complement(2867..3067)
                     /locus_tag="VI541_03865"
     CDS             complement(2867..3067)
                     /locus_tag="VI541_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLF70074.1"
                     /translation="MSSGTVKWFSAEKGYGFISRDDGEDVFVHFSSIEGAGYRNLEEG
                     QKVEFEVTQGPKGLQASTVRLV"
BASE COUNT          603 a          975 c          959 g          624 t
ORIGIN      
        1 ccgcttcagc atcaccggct tgccggtcga gccgatctcc ttcagaagcg ggaagttctg
       61 catgttgcgc gtcccaacct ggagcatgtc ggccacggcc gcgaccttgt ctacgtcacg
      121 cgcgtccatg acctcggtca cgatgggaag ccccgtctcc tgccgagccg tttcaaggat
      181 cttcagccca tcctcggcca gaccctggaa cgaatacggc gaggtccgag gcttgtacgc
      241 gccgccgcga aggatgtgag cccccgactt cttggctgcc atggccgccg cgatggtctg
      301 ctcctcgttc tcgacggcgc atggtccggc gatgatggtg aatgtatcgg gccctatcga
      361 tgccgagccc accgcaacgg tcgaggagcc ggggtgcccc tcatgcgaga cgaccttgta
      421 cgacttggtg acgcgttgaa cgcgctcgac gcccctcatg gccgcgaggg gaagttcctc
      481 gagcggttcg gggtccccga cgatgccgat gaccgtgtgg aagtctccgc gcgacacgaa
      541 acactcggtt ccggtcgccc ggaccttgct cagtacagac tcgacctcgt cggtagtcga
      601 gtccttccgc atcacgatca gcatggtcag agcgtatccg aaatcccgtg gtctccatgg
      661 aggtcgtgct acacaagggt ctgatgtcct acacgcgaag agcggtccac tgggatgcag
      721 atacccgcgg tcgtcgggat ttcgaacgac aggtgcgcca gacgatcaag gatgacggtg
      781 agttggtcta tctcgactcg acggattcgt tcgacgagat cgacttcgag ctgggctgca
      841 gggggcgccg gctcttcgtg gaggtcaaag agaagctgca acactatcgc gcggcttggc
      901 aggaggcctc ggcagttcca gaggaagatc tgttcatcct cgacgagttg gctgcccgca
      961 agatcatcct gcgtgctcca cgcgcctatc tgttgatcca cgatgccatc tgcgaacgcc
     1021 tatccgtctt cggcgcgctg gaacttctga cagcacccaa ggacaggctg gaccgttcga
     1081 tcgaggccaa tgtcgcgacc ttcaagggga agtggctcat agacctgctt catgggttcg
     1141 agtgcgcgtc cttggcggag gccctcgact acatcaagag aaggtcgacc tccgaggaag
     1201 atgcatggca ggggctcgcc tgccacggtc gttaccgcga tgagcatctg cctcgcatct
     1261 gaacctgaaa ccaccgtgct cgtctaaact ggatcgttcc ccaccgtctg agagacatgg
     1321 cgatatgaag aagaccggtc gagagatgct ggcagaggcc cggcgcaagg ctgccgcgct
     1381 cgaacccgaa caggtcgatg agatccgccg caacgaagac gtgctgctgg tcgatgtgcg
     1441 cgagtcggat gagtggcgag ctggccacct tccgggagcc atccatcttc cgcggggctt
     1501 cttcgaacta cagatcgacg agaaacttcc cgacaagacg aagccggtaa tcctctactg
     1561 cgcggggggc cttcggtccg ctcttgcggc gatgaccatg gagcagctcg gatacaccga
     1621 tgtccgccac atgaacggtg gcttcacgcg atggcgcgac ggcggattcg aagtggtggc
     1681 cgaccggcag tggaccccgg aacagcgcga cagatactca cgtcacttca tccttcccga
     1741 ggtgggggag gccggacagc agaagatctc gtcgggaagc gttctggtcg tgggggccgg
     1801 gggcctgggt tcgccggcgc tctactacct cgcagctgca ggcgtcggtc ggctgggcat
     1861 cgtggacttc gatgcggtcg agcgcaacaa cctgcagaga cagatcatcc actcgaccgc
     1921 gcgtatcgga acgaacaagg ccgagagcgc agccgcgacg atcgtggatc tgaatccaga
     1981 cgtgaaggtc gagacctgga cagagcggat cacgcccgac aatgtcgata gcatcgtcga
     2041 tggattcgac gtcatcatcg atgcgacgga caacttcgtt actcgataca tgctcaacga
     2101 cgctgcggtc cgtttcgcca agccctacat ctacggctcc atattcagat tcgagggtta
     2161 cgcgagtgtg ttctggccag ccgagggggg gccctgctac aggtgccttc accccgaagc
     2221 gccgcccgag cacctcgccc ccacctgagg cgaggctggg gtcctgggcg tgctcccagg
     2281 gatagtcggt tcgatacagg cgaacgaggc cctcaagatc ctcgtcgggt atggggagcc
     2341 tctcgtcggg agactgctca ccttcgatgc ccatgacacg aggttcggcg agctgaagct
     2401 ccgtcgcctt gctgactgtc cgacctgcgg cgggaaatag aagagccccc cgaagggggc
     2461 ccctcttaga acgaacgact ctctagcgtt tcctctgtgg acgataactc ccgcgtcgcc
     2521 gttgctgccc tggtttagga cgtttgcgaa cgggtctcgc gctctgagat gggtcctgac
     2581 tcgggaatgg ctggcggttg gccagcgaca gagcgacagc atcggagact tcttctctcg
     2641 cgagatcgct tgtctcggtt ccgaccatca attggaacag aacgcttctc tccaactgct
     2701 cgatcgaatc aactaccttc tgtcgggttc ccgcctgtgt cttccactca gcgtggatcc
     2761 gacctgaggg tggaatctct atcttgaggt cggcatcgcc gaagcggcct tcccatgcag
     2821 tagtgctaga actcaaaagc cgaactcctt gttgcggttg cgtcgtctag acgagacgaa
     2881 ctgtcgacgc ctgaagtccc ttaggaccct gggtcacttc gaactcgacc ttctgacctt
     2941 cctcgagatt gcggtatccc gcgccctcta tggaactgaa gtggacgaat acgtcctctc
     3001 cgtcatcgcg cgagataaag ccatagccct tctccgcgct gaaccacttt acggtgccac
     3061 tagacatgcg attccctccc ttcctaacaa cttgccggcg aaccggcggt atggagggaa
     3121 ctgctggtac cgcttgctga gcatgaacct ctgccgtgcc c
//
LOCUS       DATDOZ010000194         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3859341, whole genome shotgun sequence.
ACCESSION   DATDOZ010000194 DATDOZ010000000
VERSION     DATDOZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859341"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /gene="msrB"
                     /locus_tag="VI541_03870"
     CDS             <1..393
                     /gene="msrB"
                     /locus_tag="VI541_03870"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLF70075.1"
                     /translation="YEVSRSENEWKEVLGPSKFAVLREAATEPPFTGSYLHNKAEGTY
                     MCGACGAVLFTSKTKFDSGSGWPSFTEPADLENVELRDDKSHGMVRTEVLCRRCGSHL
                     GHVFPDGPGPTGERYCINSCALEFAPGE"
     gene            complement(399..875)
                     /locus_tag="VI541_03875"
     CDS             complement(399..875)
                     /locus_tag="VI541_03875"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HLF70076.1"
                     /translation="MNGLYVKILLLIHIMGAIIGLGPTMVFGILGPRAKAAGPNGGVA
                     IMESMVAIERALVFPVAMITQPVSGVLLIFATDLNQGFFNHIWLWVAIILFIGILSLS
                     FGVNNPALHKMIDLAKGGKAMEPEFASLAAKVQRVGPLIVLMFLTVIFLMVMKPGG"
     gene            996..1916
                     /locus_tag="VI541_03880"
     CDS             996..1916
                     /locus_tag="VI541_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF70077.1"
                     /translation="MSVVKARALTKKFGDFTAVDAVDFDVEPGEAFGFLGPNGAGKTS
                     TMRMIACTSPVTAGELKVLGRDPRRDGSAIRGFLGVVPQTDNLDTELTVRENLEMYAR
                     YFDISRAVTKERVDELLDFVQLSERCNDPVEDLSGGMKRRLTIARALINEPELILLDE
                     PTTGLDPQARHLLWDRLYRLKQRGATLVITTHYMDEAEQLCDRLVVMDAAKIVAQGTP
                     RELIERYVTREVLELRVSDETRSTQQGVFHRVADRVEALPDRVLLYTQDGEKTLAEIH
                     SQGVITESALVRRATLEDVFLSLTGRSLIE"
     gene            1913..>2272
                     /locus_tag="VI541_03885"
     CDS             1913..>2272
                     /locus_tag="VI541_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HLF70078.1"
                     /translation="MRFRKVWIRNGLVYRRRWRGSFIGSFIYPLLLLSAMGLGLGRLI
                     SQNRPGSFGAGGYLGFVASGLIAAVCMQSTVFEASFPMLDRIKDRRTFDAILATPLGI
                     RDILFGEMAWLATRASTV"
BASE COUNT          446 a          668 c          700 g          458 t
ORIGIN      
        1 tacgaagtgt cgcgaagtga gaatgagtgg aaggaagtac taggcccgtc caagttcgcg
       61 gtcctgcgcg aggccgcaac ggaaccacct ttcacgggga gctatctgca caacaaagcg
      121 gagggcacct atatgtgtgg agcgtgtggc gcggtactct tcacatcgaa gacgaaattc
      181 gactccggca gcggatggcc cagtttcacg gagccagccg atctggagaa tgtcgagctg
      241 agggacgaca agagccacgg catggttcgc acggaggtcc tctgccgtag gtgcggatcg
      301 catctgggtc acgtcttccc tgacggcccg gggcccacag gggagcgcta ctgcatcaac
      361 tcgtgcgcct tggagttcgc acccggcgaa tagcctctct agcctcctgg tttcatcacc
      421 atcaagaaga tcaccgtaag gaacatcagc actatcagtg gaccaacccg ctggaccttc
      481 gccgcgagtg acgcgaactc tggctccatc gctttgcctc ccttggcgag gtcgatcatc
      541 ttgtgcaggg ccgggttgtt cacaccgaag gacaggctca ggatcccgat gaaaaggatg
      601 atcgcgaccc acagccagat gtggttgaag aagccctggt tcaagtccgt agcgaatatg
      661 agaaggacac ctgatacggg ctgagtgatc atggcgacgg ggaagaccag ggcacgctcg
      721 atcgcgacca tcgactccat gatcgccacg ccgccattgg gcccggccgc cttcgcccgg
      781 gggcccagga tgccgaagac catagttgga cccagcccga tgatggctcc catgatgtgg
      841 atcaacagca ggatcttgac gtagagcccg ttcatcgttt cgacccctct ccttcgacct
      901 agccgtgacg tcggcgtcgg cgattctaga acctagctga ggcgtggggt agcacttggg
      961 cgcgagggaa ccggaccttc tgtagcctgt ggtccatgtc tgtggtcaag gcccgtgctc
     1021 tcaccaagaa gttcggcgac ttcaccgccg tcgacgcggt cgacttcgat gtggaacctg
     1081 gtgaggcctt cgggttcctg gggccgaatg gcgcgggtaa gacctcgacg atgcgcatga
     1141 tcgcctgcac ttcgccagtc accgctggag aactcaaggt cttgggccgc gatccgcggc
     1201 gagatggatc ggcgatccgc ggattcctcg gagtcgttcc ccagaccgac aatctcgata
     1261 ccgagctaac ggtgcgcgag aaccttgaga tgtacgcgag atacttcgac atctcgcggg
     1321 ccgtgaccaa ggagcgcgtc gatgaactcc tggacttcgt tcagttgagt gaacgctgca
     1381 acgaccctgt cgaggacctg tccgggggca tgaagcgccg cctcaccata gcgcgagcgc
     1441 tgatcaatga gcccgaactc atccttctgg atgaacccac gactggtctt gaccctcagg
     1501 cgcgccatct cctttgggat cggctctatc gtctgaagca gcgcggagcc actcttgtga
     1561 tcacaaccca ctacatggat gaggccgaac agttatgtga tcgactcgtg gtgatggatg
     1621 cggccaagat cgtcgctcaa ggtacgccgc gggagttgat cgagagatat gtgacccgcg
     1681 aggttctgga gctgcgggtc agcgatgaga ctcgcagtac gcagcagggc gtcttccaca
     1741 gggtggctga cagggtcgag gcgcttcctg accgggttct gctctatacc caagacgggg
     1801 agaagactct cgcagagatc cactcgcagg gagtgataac cgagagcgcc ttggtacgca
     1861 gagccactct cgaggatgtg ttcctcagcc tcaccggccg gtctttgatc gagtgagatt
     1921 ccgaaaggtc tggatccgca acggtcttgt ctaccgtcgg cgctggcgcg ggtcgttcat
     1981 cggttccttc atctacccgc tccttctgct atcggctatg gggctcggcc ttggcaggct
     2041 gatctcacag aatcgtcctg gatcgttcgg cgcgggaggg tatctgggct tcgtcgcatc
     2101 gggtcttatc gctgcggtct gcatgcagtc gactgtcttc gaggccagct ttcccatgct
     2161 cgacaggatc aaggaccgtc gcaccttcga cgcgatcctt gccacgccgc tggggatcag
     2221 agacatcctc tttggtgaga tggcctggtt ggccactcga gcatcgaccg tc
//
LOCUS       DATDOZ010000195         3976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1744584, whole genome shotgun sequence.
ACCESSION   DATDOZ010000195 DATDOZ010000000
VERSION     DATDOZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744584"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VI541_03890"
     CDS             complement(<1..916)
                     /locus_tag="VI541_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HLF70079.1"
                     /translation="MRKAMVITAVLGALLSGQAPHRAYIRNVEILALVAPSGSLTVAE
                     RITVEPLATSARIEYPQPGGWAYSDWTLMDAATASPVDGHLTSPTIIRTKRDRIVAGW
                     RFAPSESQRTFDVTYRVEGAVIRHLDVAELRWERFMVSEQEMGVARIQLSLPSPGGSR
                     PNVRAWAHGSIDQEIGQQSKRDIVAHVATIPAGQHLGVRVILPRRLVPRAREISDRVR
                     PGIIATEEHGVASAASRLRRAETDMAHATSVTLLVIIGIALIAGVSAIPRLRGPLASL
                     INFGVGSGAAVFGWRSFATSGLPAGLLLM"
     gene            1034..2353
                     /locus_tag="VI541_03895"
     CDS             1034..2353
                     /locus_tag="VI541_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70080.1"
                     /translation="MGFLLAAVPLGILIGLWIENAVNVPYWDEWAFIPLLQKLNSSGV
                     AFSDLWAQHNEHRIFLPRTIMLAMGSLTHWDVRYEMAASLALAVGIFSLLFLLLSRTF
                     EKRRYPFGIAGLVLGLAIFSPAGYENWLWGWQIQWYLNVLALVAVVVLLSSKLGERLP
                     FVAMSLAALGAVFGTLSLASGNFIWVAAAPMILFQRHLRRYAWLFFGIGMISVGTYLR
                     GYHQTNSSSGVDYLLHHGGDLMSFIVQYLGRPFSSSLGVATVAGSILLATFIVVLLIL
                     IVGQRDQFARWGPWMCIAMYVGIADLSTGVGRLTLGGYPGLSSRYTSISALMIPSVVA
                     CLSVLLESFKRTRGVTAHVLLWGLVGFGLLTSYRASIGDFELRGEQMGLIKECVESAS
                     FEGVRCLRQAYPDEHTVYWRGRYLMQIGWGGFEMSTRVPGPRVSRTP"
     gene            complement(2313..3506)
                     /locus_tag="VI541_03900"
     CDS             complement(2313..3506)
                     /locus_tag="VI541_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70081.1"
                     /translation="MNICTIVASNYIPQARVLASSLARVHPELRLTTLIVDPMEPPPS
                     EPFEVIFPEALGLSLKNIHHLAMIYTPVELCTALKPHLLSWVLRQGEPAVYLDPDMEV
                     FARLDEVVPMTARHDIVLTPHVTEPIPRDGLRPTELHILEAGVYNMGFIAVGRGAQDF
                     LDWWAERLLTDSIVDPRNTLFVDQRWVDFVPALWEPFLLKDPGYNVAFWNLHSRKIEK
                     GPEGLTVNGRPLRFFHYSAFDPRRPGELSKNQGDMPRVRLSERPDVKELTDRYAAALI
                     SAGYDEAIGRTYGWARTPQGNLITVPMRRAYRKGVVEAQDLASRPPNPFDVDEETFVS
                     WLAQPVELSMPAVSRYLAAIYEERADVRGAHPGVMQGNPESFIGWVKEHGVKEYEIPG
                     GLVPE"
     gene            complement(3503..>3976)
                     /locus_tag="VI541_03905"
     CDS             complement(3503..>3976)
                     /locus_tag="VI541_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HLF70082.1"
                     /translation="HRRQGTGEAVIEHYELFVDNHENRVLRSGGPTSVRIFYDASVSI
                     PDPCFVLRYTSPDGRVIAETSTDEHALSLGPIFGKGSLEYQIPELRLVPGTYDLTASL
                     TDRTGEKLFDRRDREIRFDVGQGALPDRNGLLSMGGRWKLLAKDAEGPIERTSAP"
BASE COUNT          730 a         1135 c         1241 g          870 t
ORIGIN      
        1 ccatcagcag aagccccgct ggtagccccg acgtagcgaa gctccgccat ccgaagactg
       61 cagccccgga cccgacgccg aagttgatca atgaagcgag aggacctcgc agccttggta
      121 tggccgatac tccagcgatc agcgcgatgc cgatgatcac cagcagagtc acgctggtgg
      181 cgtgggccat gtcggtctcg gctctcctca gccgggatgc tgccgatgca accccgtgtt
      241 cctcggtcgc gatgatcccc ggacgcaccc ggtcggatat ctctctcgcc cgagggacga
      301 ggcggcgagg caagatcact ctgaccccaa gatgctgacc cgctgggatc gttgcgacgt
      361 gggcgactat gtcgcgttta ctctgctgac cgatctcctg atcgatggag ccatgcgccc
      421 atgcgcggac gttggggcgg gatcccccag gcgagggaag gctcagctgg atacgtgcga
      481 cccccatctc ctgttccgaa accatgaatc gttcccagcg cagctccgcc acatcgaggt
      541 ggcggatgac tgcaccctct acacggtagg tgacatcgaa cgtccgctga gattctgatg
      601 gagcgaatcg ccaaccagcc actatgcggt cgcgcttggt cctgatgatg gtgggcgagg
      661 tgaggtggcc gtcgaccgga gatgcggtcg cagcgtccat aagagtccaa tccgaatagg
      721 cccagccccc gggttgcgga tactcgatcc gtgcggaggt cgccaaaggt tcgacggtta
      781 tcctctctgc gacagtcagg ctccctgatg gtgccaccaa ggcaaggatc tcgacgtttc
      841 tgatgtacgc gcgatggggg gcctgaccgc tcagcaaggc accgagcacc gccgtgatta
      901 ccatcgcctt gcgcatgcgt ttccctcgga tcgcccgatg cctataccct ctcctggctg
      961 atgagcaaac gccgaggcaa gggaccgtgt gaacgctagc cgaaagccgg caagagtgtc
     1021 gtcccaacgg gctatgggct tcctcttggc agcggtaccg ctggggatcc tgatcggttt
     1081 gtggatcgag aatgcggtca atgtccccta ctgggatgag tgggcgttca ttccactcct
     1141 gcaaaagttg aactcatcag gtgtcgcgtt ttccgatctg tgggctcaac acaacgaaca
     1201 tcgcatcttt ctacccagaa ccatcatgct tgctatgggg tcgctgacgc attgggacgt
     1261 gcgctacgag atggcagcga gcctcgctct tgccgttggc atattcagtc ttctgttcct
     1321 cctgctctcg cgaactttcg aaaagaggag atatccattt gggatcgcgg ggctggttct
     1381 cggactggcc atcttctcgc cagccgggta cgagaactgg ttgtgggggt ggcagatcca
     1441 gtggtatctc aacgttctag cgctggtcgc agttgtggta ttgctctctt cgaagttggg
     1501 cgaaaggctc cctttcgttg cgatgagtct cgccgcacta ggcgcggttt ttggaacact
     1561 gtcattggcg agcggtaact tcatctgggt ggcggcagcg ccgatgattc ttttccagcg
     1621 gcatctcagg aggtacgcct ggctcttctt cggcatcggg atgatcagcg ttgggacata
     1681 tctgcgcgga tatcaccaga ccaactcctc atcaggtgtt gactatcttc tccatcacgg
     1741 tggggacctg atgtccttca tcgtccaata tctgggcagg cctttcagta gctctcttgg
     1801 cgttgcaacg gtcgcgggaa gcattcttct ggctaccttc atcgtggtcc tgctcatcct
     1861 tatcgtggga caaagagatc agttcgcgag gtggggaccg tggatgtgta tcgcgatgta
     1921 cgttgggatc gctgatctct cgacaggagt ggggcgcctc acactcggag gttacccggg
     1981 tctcagcagc agatacacgt cgatctcggc gttgatgatc ccttctgtcg ttgcatgcct
     2041 atctgttctt cttgagtcct tcaaacgaac gagaggggta acagcgcatg tcttgttgtg
     2101 ggggctggtg ggttttggac ttctcacaag ctaccgtgcg tccatcggag acttcgagct
     2161 tcgcggagaa cagatggggt tgatcaagga gtgcgtcgaa tcggcgtcgt tcgaaggggt
     2221 gcggtgtctc aggcaggcat atccagatga gcacacggtg tactggcggg gccgctacct
     2281 catgcagatc ggctggggag gtttcgagat gtctactcgg gtaccaggcc cccgggtatc
     2341 tcgtactcct tgaccccatg ctccttgacc cagccgatga agctctcggg gttgccctgc
     2401 atcacaccag ggtgtgcccc cctcacgtcg gccctttctt cgtaaatggc cgcgaggtac
     2461 cgggaaacag ccggcatcga cagctcgact ggctgagcga gccaggacac gaacgtctct
     2521 tcgtcgacgt cgaacgggtt cgggggccta gatgcaagat cttgcgcctc caccactccc
     2581 ttacggtagg cccttcgcat cggaacggtg atgaggttgc cttggggggt cctcgcccat
     2641 ccgtaggtcc ggccgatcgc ctcatcgtat ccagcggaga tgagggcggc ggcgtaacgg
     2701 tcagtcaact ctttgacatc aggcctctcg ctcaatcgca cacggggcat gtcaccctga
     2761 ttcttgctga gctcgccagg ccgtcgaggg tcgaaggcgc tgtagtggaa gaatcgaagc
     2821 ggccgcccgt tcaccgtcaa cccttcggga cctttctcga tcttgcggga gtgcaggttc
     2881 cagaaggcga cgttgtagcc ggggtctttg agaaggaatg gttcccagag agcggggacg
     2941 aagtccaccc atcgctgatc cacgaaaaga gtgttgcgcg ggtcgacgat gctgtctgtt
     3001 aacaggcgct ctgcccacca gtcgaggaag tcctgagctc cacgtccgac ggcgatgaag
     3061 cccatgttgt agacgccggc ctcgaggatg tgtagctcgg tcgggcgtag accgtctcgc
     3121 gggataggtt ccgtgacatg tggcgttaga acgatgtcgt gtctagcggt catcggcacg
     3181 acttcgtcca aacgagcgaa tacctccatg tcggggtcca gataaaccgc tggctctcct
     3241 tgacggagca cccaactgag gaggtggggt ttcagggcgg tgcaaagctc caccggggta
     3301 tagatcatcg ccaggtggtg gatgttcttg aggctgaggc cgagcgcttc ggggaagatg
     3361 acctcgaatg gctcgctcgg tgggggctcc atcggatcga cgatcagcgt agtgagcctt
     3421 agctcggggt ggaccctggc cagcgacgat gcgaggacgc gcgcctgggg gatgtagttg
     3481 ctcgcgacga tcgtgcagat attcaaggag ctgatgtccg ctcgatcggg ccctcggcat
     3541 cctttgctag gagcttccag cgaccgccca tgctgaggag accgttgcgg tcgggaaggg
     3601 ctccttgtcc gacatcgaac cggatctctc gatcgcggcg atcgaatagt ttctctccag
     3661 tccgatcggt gagcgatgcg gttaggtcat aggttccagg gacgagccgc agttctggga
     3721 tctggtactc caggctcccc ttgccgaaga tgggtcccag gctcagagcg tgttcatctg
     3781 tcgaagtttc tgcgatcacg cgtccatctg gactggtgta tcgcagcacg aagcaggggt
     3841 ctgggatcga gacgctagca tcgtagaaga tccgcaccga agtcgggccc ccggatcgaa
     3901 gcactcggtt ctcgtgattg tcgacgaaga gctcatagtg ttcgatgacc gcctctccgg
     3961 taccttgtcg gcgatg
//
LOCUS       DATDOZ010000196         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3037318, whole genome shotgun sequence.
ACCESSION   DATDOZ010000196 DATDOZ010000000
VERSION     DATDOZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037318"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VI541_03910"
     CDS             <1..787
                     /locus_tag="VI541_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70083.1"
                     /translation="YDPSTIETIFELDAVAKDAGVMVIPGIGWTPGLTNMMVKHAAAS
                     LDHVRSARISWVASISSLHEETFVRIARALSGTSVVLSGGGWARRSAGEDEEEIFFPP
                     PIGWRQVRLAENWEAQSLPRAIEGIEDVVVKAGITEAALDSLARAVSAIPALSSRDGR
                     TRMAPILGPVVPMLGRLSGARHSWSAARVDVTGTRHGGPASISLAVLDQTTNLLCAPV
                     VAATLAVAGHGSAGVHSPESVLDPPAFFRSIAQAGVRIAKLNR"
     gene            820..892
                     /locus_tag="VI541_03915"
     tRNA            820..892
                     /locus_tag="VI541_03915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:853..855,aa:Ala,seq:tgc)
     gene            complement(1047..2435)
                     /locus_tag="VI541_03920"
     CDS             complement(1047..2435)
                     /locus_tag="VI541_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70084.1"
                     /translation="MRTSEMKYLSALAGALLVLGVVASPSGADPLREGTSALISRYPD
                     GSQGEGGYPSVSADGRYVAFESWSALLPEDTDSDTNIFTDNNSDIYRYDRLTGEILLV
                     SVNYPSVSFASACNYRPSISGDGSKVAYRSGALFGGSEYGDRACYAGHVVVRDIPSTT
                     SKAIPGTYGGWTTQGMKMSRDARYVAVIRSDGFDDHFAAVIDVASGSLRWESPVVCVE
                     CLTAIMDDSISISGNGRFVAYDARAGQYAGDPIVLWGTYLVDRDTNNNGVFDDSGNSL
                     TNVAILPSGAQVRWSRSNAVSDDGKLVAFISANTNTMQDNTFKYYLRNLTVGTTEILS
                     QSSIGVPGAGADPRFNWPVGISPSISADGRYVAFSTMDPYLAPGDTKPTLDVFVRDRL
                     TGKTHLASALPGGDQDYLDAVAPALSADGNYLAFDTQLAPYQIHNNYFRSGYLREVWT
                     PCSIACLPNPSL"
BASE COUNT          526 a          814 c          860 g          542 t
ORIGIN      
        1 ttacgaccca tcgacgatcg agaccatctt cgaactcgat gcagtggcga aggatgcagg
       61 tgtcatggtc atccccggca tcggttggac tcccggtctc accaacatga tggtcaaaca
      121 cgcggccgct tcgctcgacc atgtgaggtc cgcgcggatc agttgggtcg catcgatctc
      181 atcgcttcac gaggagacat tcgttcgtat cgcgcgggct cttagcggaa cgtccgtcgt
      241 gctatctggt ggaggatggg cgcggcgctc ggctggtgaa gatgaggagg agatcttctt
      301 tcccccaccc atcggttgga ggcaggtaag actcgccgag aattgggagg cgcagtcact
      361 gccgcgagcc atcgagggca tcgaggatgt tgtggtgaag gcggggataa ccgaggccgc
      421 cctcgattca ctggcacgcg cggtgtcagc gatacctgca ctctcttcgc gcgacggtag
      481 gacgcgtatg gccccgatcc ttgggccggt tgttccaatg ctaggtcgac tgagtggtgc
      541 gcgtcactcg tggtccgcgg cacgcgtaga tgtcacaggt acgcgacacg ggggcccggc
      601 ttcgatctcg ctcgccgtgc tcgaccagac caccaacctg ctatgtgctc cggtggtagc
      661 agcgactctt gcagtggcgg gacatggcag cgccggggtt cactcccccg agtcggtgct
      721 cgatccgcca gccttcttcc gctcgatcgc ccaggctggc gtcaggatcg cgaagctcaa
      781 ccgctgagtc tcgaggatca actacaatga gcgcttctgg gggccgtagc tcagttggta
      841 gagcgccgcc tttgcaaggc ggaagtcgtc ggttcgagtc cgatcggctc cacgatgaga
      901 aagtgcaggc cagtggctgt ttcgaggcca agacgagata tggtcgggtc tggttccaac
      961 tctgtggccg accttcggta ttcgtgatcg caacaaaaac ccccggaatc cttgtgcgtt
     1021 cccgttgcgg agcccaacca cgtggatcag agcgatgggt tggggaggca ggcgatcgag
     1081 cagggggtcc aaacctcccg caggtaccct gaacggaaat agttgttatg gatctgatag
     1141 ggggctaact gagtgtcgaa ggccagatag tttccatcgg cggagagggc cggcgccacc
     1201 gcgtcgaggt agtcctggtc cccgccgggg agggccgatg ccaggtgggt cttcccagtc
     1261 aatcggtccc tcacgaagac atcgagtgtc ggcttggtgt ccccgggggc gaggtaggga
     1321 tccatggtcg agaaggcgac gtatcgtcca tccgctgaga tcgaagggga gatgccgacg
     1381 ggccagttaa agcgggggtc cgccccagct ccggggaccc cgatcgaact ctgagagaga
     1441 atttcggtcg tgccgactgt caggttgcgg aggtagtact tgaaggtgtt gtcctgcatc
     1501 gtattggtat tggcactgat gaaagcgact agcttcccat cgtctgacac cgcattggat
     1561 cgactccacc gcacctgggc ccctgacggc agtatcgcca cgttcgtgag tgagttgcca
     1621 gagtcatcaa acactccgtt gttattggta tctctatcca ctaggtaggt cccccagagg
     1681 acgatggggt cccctgcgta ctgccctgcc ctggcatcgt aggccacgaa cctgccgttg
     1741 ccggagatgg agatggagtc gtccatgatc gccgttaggc actccacgca gaccaccggg
     1801 gactcccatc ggagagagcc gctggcaacg tcgatcaccg ccgcgaagtg gtcatcgaac
     1861 ccgtccgaac ggatcacagc gacgtatcgt gcatcccgcg acatcttcat cccctgcgtc
     1921 gtccacccac cataggtccc ggggatagcc ttgctggtag tgctgggtat gtccctcacc
     1981 accacgtgac ctgcataaca tgctcggtcc ccatactcgc tccccccgaa tagcgctcca
     2041 gaacgatagg ccaccttgct cccgtcccca gatatcgagg ggcggtagtt gcaggcgctt
     2101 gcgaagctca cggagggata gttcaccgaa accaggagaa tttcaccggt cagccgatcg
     2161 tagcggtaga tgtcgctgtt gttatcggtg aagatgttgg tgtcagaatc ggtgtcctca
     2221 ggcaggaggg cggaccaaga ctcgaaagcc acgtagcgac cgtcggctga gaccgagggg
     2281 taccccccct cgccctgtga gccgtcgggg tagcgggaga tgagcgcgct cgtcccctcc
     2341 cgaagagggt cagctccgga gggagaggca accaccccca atacgagcag ggcaccggcg
     2401 agcgcgctca agtacttcat ctcactggtt ctcattcgac gccgaggcca aagagtcctc
     2461 cccccatctg agaaagatgt tgacttgttg gccaatcggg aaaagcattc cataccgggc
     2521 tacgtgatga ctaggggggc gacaatgtct aagcgatcgg ccatctcgag gatcgttgcc
     2581 ctcacgctct gctctcttac tgcgcccggc tttcgccccc ggcaacatca tcgtcgacgc
     2641 caacagtgat gtcttcaata tcggctctgg aggcggcgga ggcgggtggg gttactcgca
     2701 agcggcgacg cagatctcct ccacggtcct cagccagatc ta
//
LOCUS       DATDOZ010000197         8258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3332817, whole genome shotgun sequence.
ACCESSION   DATDOZ010000197 DATDOZ010000000
VERSION     DATDOZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332817"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VI541_03925"
     CDS             complement(<1..752)
                     /locus_tag="VI541_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70085.1"
                     /translation="MWATTGSYLLTRTALTDAQSRVDGELQSQMRNAGVGFSDLVIRR
                     IELVRLMSHTQGVPEALKANDFNTLRSRLGPLLLNSRADALLILDAADEEKFALWRNA
                     SELEVGAVGLPNEDRRKIGSVARSTVSDKSVLLSRSTKGPLVLTTSVVRFKGAYVGSL
                     AVGNLSSSLVARLGESAVIVDKAGKSQEPAVALYDAQGNFIDSNKPVVMLTKDAVVHP
                     RSSHESVGDRPVLVGELVANGAVVARLAIFHEG"
     gene            complement(821..1594)
                     /locus_tag="VI541_03930"
     CDS             complement(821..1594)
                     /locus_tag="VI541_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70086.1"
                     /translation="MRTRSQLPIAFLMAGAAVVALFGSVTLALGGKQPRTASVVGANL
                     PPFLFGKDGEEADVSPVDALGVIQDIAGENLEPLAQGSQSGVTTEPSVTPGGGGGGGG
                     GQEPKPSMSLTMTARTRHLGGSRQGSYEIAYDSIVKNTGSTNLRAITFTSHVPAGTQW
                     TPNADCSGNGYAITVAYDGGSARIFCVPGSTPIAGSDDPSIHPVRLTLDQSLAPGSVA
                     TISFSVQIAAPRDSDVVNHSHSEVPGLRVDSLDVTTRFV"
     gene            complement(1584..1910)
                     /locus_tag="VI541_03935"
     CDS             complement(1584..1910)
                     /locus_tag="VI541_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70087.1"
                     /translation="MSTISEMEARVRHDIRHPIATVSMIASAISEFGNSVEPDQFFAY
                     CRQIEIEISLLQRLLKEHDVELDTSDLESLGRSFLDEPRDVARAAKLDHECRRLIEVL
                     GGTDAH"
     gene            1974..3044
                     /locus_tag="VI541_03940"
     CDS             1974..3044
                     /locus_tag="VI541_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLF70088.1"
                     /translation="MPTIRVLVVDDTPHLRAVVGTILGSAPGFELVGEAEDGEEAVQM
                     AARLKPDIVLMDVEMPGLNGLAATAAIVKSHPEIKVVAWTNHVEPALITEMVAAGAAG
                     YLLKGIQPREFLASMASVAAGASVLSPEVTSTVVQELARLYRRAQSKADELAESYLNT
                     VQALAAALETKDDQTGNHARRVRDYATIVTAHYDPALLESESLIFGFLLHDVGKIGIP
                     EHILMKPGPLTENEWKIMRRHPELGAKIVGSAPFLTTDAVQVVSAHHERWDGSGYPNR
                     LAKDQIPVGARLFSVADALDAITSDRPYRLGLPFEAAVAEIERCSGSQFDPEVFSAFI
                     DGLPEIEARYRSEVSDIATTAS"
     gene            complement(3049..5565)
                     /gene="secF"
                     /locus_tag="VI541_03945"
     CDS             complement(3049..5565)
                     /gene="secF"
                     /locus_tag="VI541_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLF70089.1"
                     /translation="MKNTRRHVISIVLTAVLIATLFGTIVGLGLSPKLGLDLRGGLAL
                     TLTGPEETRSDVLDKTVEILRRRIDAAGVAEPEIAREGKNNISIQLPGSGDSRRLLAL
                     IGRTAQLQFRQVQQTLPRGTPQFASATVTLADDPAASVVLLSKDGNEKLQLGPAELTG
                     EVVKRGVAIIDQGDTGSVGGWQVQLSFTGEGAKKWKEFTGRLACQQGDERQIAIVLDQ
                     DVESHPQVATDVQCNEGIANGNTVITGNFTEREAKELALVLTTGALPVKLEQSEVRIV
                     SPTLGKDSLRAGLLAGALGLALVFLYVLAYYRTLGLQTWLGLVAFSSIVYGLIVGFGA
                     LIGWNLTLAGIAGLIVSIGIATDSYIVFFERVKEEVHEGRTLPSSIDKGFKHAWRTML
                     TANMVTILAAVVLYLLTVGSVRGFALALGMATTIDLAVTYALTWPLASLLARSSFFGE
                     NKVFGMRRALEGNPKKKGGLLAKIYRSEFAVDFIGRRRIWLTISAVVVGLSIVIVLPG
                     VRGLTYGIDFKGGNVFRAPLSNGLEADPIKSALREEGVSDAVVQVIQDRVSKNREVEV
                     QTEADKDPAKNAIIRTKVADALAKVTGAPVADIATDSVDKKWGAQITAKALRGLVVFL
                     VLVILYMSWRLEPKMAIAGIVALLHDVLITIGVYALVGFEVSPATVIATLTILGFSLY
                     DTVVVFDKIRENQALSVNARKSFGEIANDSTNQVLMRSINTSLTVLLPVGSLLFVGSA
                     LLGATTLKDLSLALFVGVAAGTYSSIFVATPLLSIWKEKEPRYQNIRAKVLRPTESAP
                     PEPVGATTSGSTEPLTTRSSPQVKPSGPRRNVSRAKRKKR"
     gene            complement(5565..5867)
                     /gene="yajC"
                     /locus_tag="VI541_03950"
     CDS             complement(5565..5867)
                     /gene="yajC"
                     /locus_tag="VI541_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLF70090.1"
                     /translation="MSSYSGLVFTVLILVMAYMVLIRPQQRRLKQHQSLVSSIGVGDE
                     VVTIGGMFGTVHQLDDTTVTLEVAPQVRIKFSRQAISRRVEHEAASETEDPRGDIE"
     gene            complement(5919..6923)
                     /gene="ruvB"
                     /locus_tag="VI541_03955"
     CDS             complement(5919..6923)
                     /gene="ruvB"
                     /locus_tag="VI541_03955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HLF70091.1"
                     /translation="MSSDLLVDVSPEESDFEESLRPRLLTEFVGQEPTKEHLSIILEA
                     ARNRGESPDHLLFAGSPGLGKTTLANIIANELNATLRVTSGPALERAGDLAAILTNLQ
                     EGDVLFIDEIHRLARPVEEILYPAMEDRAIDIIIGKGPAARSIRIDLPAFTLVGATTR
                     TGLLTSPLRDRFGFLGKLDYYEPEDLHVIVERSSRILGVDIHLEAAIEIARRSRGTPR
                     VSNRLLRRVRDFAQVRASGTITHEVALRALTVFEVDGSGLDKVDLAILDTLIKRFRGR
                     AVGLSTLAVAVGEEPDTIEDVYEPFLLRQGFLLRTPRGRVATDLAYEHLGVIREDRLI
                     "
     gene            complement(6920..7489)
                     /gene="ruvA"
                     /locus_tag="VI541_03960"
     CDS             complement(6920..7489)
                     /gene="ruvA"
                     /locus_tag="VI541_03960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLF70092.1"
                     /translation="MISSLKGSVTDLSDGTVEVDVNGVGYQVACSTRTISTLRVGKVV
                     RLAVQTIVREDSISLYGFASADERELFKVLLSVNGLGAKIALTILGSLDETALRGAIA
                     SDDVELLVTVPGIGKRTAQRMIVELKGRLGAPESDEASDPRMIEAREALASLGYETSE
                     VRSALAGLDDSDLQVEQMVKQALKGLARA"
     gene            complement(7486..8001)
                     /gene="ruvC"
                     /locus_tag="VI541_03965"
     CDS             complement(7486..8001)
                     /gene="ruvC"
                     /locus_tag="VI541_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLF70093.1"
                     /translation="MSQSRGVRVLGIDPGLTRLGYAVVEEDAGRIVCLRSGTFTTSPD
                     GQASERLASLWRDICEVADGSDADSMAVERVFLNANRMSAVGAIQASGVALVVAAQRG
                     MPVFEYSPMEVKHAVVGTGSASKEQVAFMVSKITGDTATAAGPDASDAVAVAIAHLSH
                     DRVRRVIGTRT"
BASE COUNT         1735 a         2511 c         2334 g         1678 t
ORIGIN      
        1 ccttcgtgaa agatcgctag ccgtgccacg acggctccgt tggcgactag ttcccccact
       61 agaacgggac ggtctccgac gctctcgtga ctcgagcgag gatgcacgac ggcatccttg
      121 gtaagcatga ccaccggctt gttcgaatcg atgaagtttc cctgcgcatc gtagagggcc
      181 acggcaggtt cctgagactt ccccgccttg tcaacgatga ccgcagactc gccaagcctt
      241 gcgacgagac tggaagagag atttccaacc gcaagcgagc caacgtaggc tcccttgaac
      301 ctcacgacgc tagtggtgag aaccaacggg cctttagtgc ttcgagatag gagcaccgac
      361 ttgtccgaga ccgttgatcg cgccaccgat cctatcttcc gccgatcctc gttcggaaga
      421 cccacggcac ccacttcaag ctcggaggca ttcctccaaa gagcgaactt ctcctcatcg
      481 gcggcgtcca agataaggag agcatccgcc cgagagttaa gcagtagggg accaagtctg
      541 gagcgcaaag tgttgaagtc attggccttc aacgcctcgg gtacgccttg cgtatgcgac
      601 atgagcctaa cgagttcgat gcgcctgatc acaagatccg aaaagccaac acctgcattt
      661 cgcatctggc tctggagttc gccatcgacc cgagactgtg cgtcggtgag ggcagttctg
      721 gtgagcaggt aacttccggt cgtggcccac aacaaggtga ggacggcaaa aggcagcatg
      781 agtttccagc gcaggtctaa atcacggata cttcccaaca tcagacgaat cgcgttgtta
      841 catcgagcga atccactcga agcccaggca cctcagaatg agagtggttg acaacatcag
      901 aatctcgcgg ggctgcgatc tggaccgaga aactgatagt cgccacggat cctggcgcga
      961 gactctgatc cagagtcagt cgaacgggat ggatggaagg atcatccgat ccagctatcg
     1021 gagtcgaacc ggggacacag aagatccttg cggacccgcc gtcatatgcg acggtgatcg
     1081 catatccgtt ccccgaacaa tcagcattcg gagtccattg cgtcccggct ggaacatgag
     1141 acgtgaacgt gatagcgcga aggttggtcg aaccggtgtt ctttacgatc gagtcgtagg
     1201 cgatctcata gctgccttga cgagatcctc cgagatgccg cgtccgcgca gtcatcgtca
     1261 aagacataga aggcttaggt tcctgacccc caccacctcc gccaccaccc ccaggggtaa
     1321 cgctcggttc tgttgtcacc ccgctttgag aaccctgggc gagtggctct aggttctcgc
     1381 cggcgatgtc ttggatgact ccgagtgcgt cgacgggaga gacatccgcc tcttcgccgt
     1441 ctttcccgaa gaggaatggc gggaggttgg caccgaccac cgaggcagtc cgcggctgtt
     1501 tgccaccgag ggctaaggtc acggaaccga agagggctac gacagcagcg ccagccatca
     1561 agaaggcgat cgggagctgc gatctagtgc gcatcggttc ccccgagaac ttcgatcaat
     1621 ctgcgacatt cgtggtctag cttggccgcc cgggctacat cgcgaggttc gtccaagaaa
     1681 ctccggccga gagactccag gtccgaggtg tcgagttcaa cgtcatgttc ttttagcagc
     1741 ctctggagta acgagatctc gatctcgatc tgcctgcagt aggcgaagaa ctgatcgggt
     1801 tcgacgctgt ttccgaactc gcttatcgcc gatgcgatca tggaaacggt cgcgatcgga
     1861 tgtcggatgt cgtgccttac tcttgcttcc atctcgctga tagtcgacac cgcccctttt
     1921 tctcttaaac tgctacccgg ggaacatccc tcaaactaac gaaagaatcc cggatgccca
     1981 ccataagagt cctcgttgtt gacgacactc cccatcttag ggcagtcgtt ggaacgattc
     2041 tcggctcagc ccccggcttc gagctcgtcg gagaagctga agacggggaa gaagcagtgc
     2101 agatggccgc taggcttaag ccggacatcg ttctcatgga cgttgagatg ccggggctga
     2161 atggactcgc tgcgacggcc gcgatcgtga aatcacaccc cgagatcaaa gtggtcgcct
     2221 ggacgaacca tgtagaaccg gcgttgatca ctgagatggt cgcggcaggg gcggctggct
     2281 accttctcaa gggcatccag ccgagagagt tcctagcctc gatggcttcg gtagccgcag
     2341 gtgcctccgt gctatcgccc gaggtaacct cgacagtcgt acaagaactc gcccggctct
     2401 atcgaagggc gcagtcgaag gcggatgaac tcgcggagag ctacctgaac actgtccaag
     2461 ctcttgctgc cgcgctcgaa accaaggatg accagaccgg aaaccatgct cgccgggtgc
     2521 gcgactatgc gaccatcgtt accgctcact atgatccggc actcctcgaa agtgagtcac
     2581 ttatcttcgg gttcctgctg catgatgtcg gcaagatagg tatccccgaa cacatcctca
     2641 tgaagcccgg tcccctcacg gagaacgagt ggaagatcat gcgacgccac ccggagttgg
     2701 gcgcaaagat cgttgggtcg gcgccgtttc tcaccactga cgctgttcaa gtggtgagcg
     2761 cccatcacga gaggtgggat ggatctggct accccaacag gctggccaag gaccagatcc
     2821 ccgtcggcgc ccgactcttc tcagtggcag acgcactcga tgcgatcacc agcgatcgcc
     2881 cctatcgact cggcctgccc ttcgaggcag ctgttgccga gatagagcga tgctctggct
     2941 ctcagttcga tcctgaagtc ttctccgcat ttatcgacgg tctgcccgag atagaggcac
     3001 gctaccgcag cgaagtttcc gacatcgcca ctacggcatc ctgaggttct aacgtttctt
     3061 cctcttggcc cgcgagacat ttcgacgggg gcctgacggt ttcacttggg ggctcgatcg
     3121 agttgtcaag ggctcggtag atcctgatgt ggtggccccg actggctcgg gcggcgccga
     3181 ctcggtgggc cgcagaacct tcgctcggat gttctggtag cggggctcct tctccttcca
     3241 gatcgaaagc agcggcgtag caacgaagat ggatgagtag gtcccagcag caacccccac
     3301 gaagagagcc aacgagagat ccttcaacgt ggtcgccccg agaagggcac ttccaacaaa
     3361 caagagactt ccgaccggaa gaaggaccgt tagtgatgtg ttgatagacc tcatgagcac
     3421 ctggttcgtc gagtcgttgg cgatctcacc gaaactctta cgagcgttga cggatagggc
     3481 ctggttctct cggatcttgt cgaataccac cactgtgtcg tagagagaga acccgaggat
     3541 ggtcagagtt gcgatcacgg ttgctgggct cacctcgaat ccaacgagcg catagacccc
     3601 tatcgtgatc aagacatcat gtagcagagc gacgatcccg gcgatcgcca tcttgggttc
     3661 cagtcgccag gacatataca ggatcacaag cacgaggaaa acgacaaggc cacggagggc
     3721 tttggcggtg atctgggctc cccacttctt gtcgacggag tcggtagcga tatccgccac
     3781 aggagctccg gtaaccttgg ctagggcgtc ggcgactttc gtccgaatga tcgcgttctt
     3841 tgcgggatcc ttgtccgctt ccgtttgaac ctcgacttcg cgattcttgc tgacgcgatc
     3901 ctggatcacc tgcactaccg cgtctgaaac accttcttct cgtagtgcag acttgatcgg
     3961 gtccgcctcc aacccattgg aaagaggcgc ccggaaaacg ttacctcctt tgaagtcgat
     4021 gccatacgtc agtccccgta cgcctggcag cacgatcacg atcgaaagac ccacaaccac
     4081 agccgagatc gtcagccaga tccttcgtct cccgatgaag tcgactgcga actcgcttcg
     4141 gtagatcttg gcgagtaagc cccccttctt tttcgggttg ccctcgagag ctctgcgcat
     4201 accgaagacc ttgttctcgc cgaagaaaga actccgcgct aacaatgagg cgagaggcca
     4261 cgtcagcgca tacgtgacag cgaggtcgat cgtggtcgcc atcccaagag ccaacgcgaa
     4321 tcctcgcacc gaacctaccg ttaggagata cagcaccacc gccgcaagga tggtgaccat
     4381 gttggccgtg agcatcgtgc gccacgcgtg cttgaatcct ttgtcgatag atgaaggtag
     4441 tgttcggccc tcatggactt cttccttgac cctctcgaag aagacgatgt acgaatcagt
     4501 tgcgataccg atcgagacga tcaaaccggc tatccctgca agggttagat tccaaccgat
     4561 aagggcgccg aaacccacta tgagcccgta aacgatgcta gagaatgcca ccagcccaag
     4621 ccaggtctga agtcccagcg ttcgatagta agcaaggaca tagaggaaga ccagggccaa
     4681 gccgagggct ccggcaagaa gcccagccct gagggaatcc ttcccaagag tcgggctcac
     4741 gatccgcact tcggactgct caagtttcac cggcagagca ccggtcgtga gaacgagcgc
     4801 gagttccttt gcctctctct cggtgaagtt ccctgtgatg acggtgttgc cgtttgctat
     4861 gccctcgttg cactgaacat cggtggccac ctggggatgt gattcaacgt cctgatcgag
     4921 gacgatggcg atctggcgtt catcgccctg ctggcaggca agtctgccag tgaactcctt
     4981 ccacttcttg gccccttctc cggtgaagga aagctgaacc tgccatccac cgacgctgcc
     5041 tgtgtctccc tgatcgatga tcgccacacc gcgcttcacg acctctcctg tcagctctgc
     5101 tgggccaagc tgcagcttct cgttaccgtc cttcgacaac agcacgacgc tggctgcggg
     5161 gtcatccgcg agggtcaccg tcgcgctcgc gaactgaggt gtgcctcggg gcagagtctg
     5221 ctgtacctga cggaactgca gttgtgcggt acggccgatc agggcgagca gtcggcgcga
     5281 gtcacccgac cctggcaatt ggatggagat attgttcttg ccctctcgag ctatctcggg
     5341 ctctgcaaca cctgcagcat cgatccttct cctcaggatc tccaccgtct tgtcgaggac
     5401 atcgcttcga gtctcttcgg ggcctgtaag tgtcaatgcc aaccccccgc gcaggtcaag
     5461 accgagcttc ggggacagcc ccaacccgac gatggtgccg aaaagcgtcg cgatcagcac
     5521 ggcggtgaga actatggaga tgacatgcct gcgggtgttc ttcactactc gatatcccct
     5581 ctcgggtcct cagtttcgct agctgcctcg tgctcaacgc ggcgggagat cgcctgtctc
     5641 gagaacttga tcctcacctg gggtgcaacc tcgagtgtca ccgtggtgtc gtcgagttga
     5701 tggacagtcc cgaacatgcc accgatcgtg acgacctcat cgccgacccc gatactcgac
     5761 acgagactct ggtgctgctt gagtcggcgc tgctgtggtc ggatcaacac catgtatgcc
     5821 atgacgagga tcagtaccgt gaaaaccaga cctgaatacg aactcatcga tcacaccttc
     5881 ctagaagtag ccgccgacaa gactaaccgc agcacgcttc agatgaggcg atcctcgcgg
     5941 ataacaccca agtgctcgta tgccaggtcg gtggcaaccc ttcccctcgg ggttcgcaga
     6001 aggaacccct gcctgagcag gaagggttca tagacgtcct cgatggtatc cggctcctca
     6061 ccgactgcca cggccaaggt ggatagccca acggccctgc cccggaaccg cttgatgagt
     6121 gtgtcgagga tggccaggtc gactttgtcg agtccgctgc catcgacctc gaagaccgta
     6181 agggctcgca gagcaacctc atgtgtgatc gtgccagaag ctcgcacctg agcgaaatct
     6241 ctgactcttc gaagcagacg gttcgatact cggggggtcc cacgagatcg ccttgcgatc
     6301 tcaatcgcgg cctcgaggtg gatatcgact cccaatatcc gtgaagatct ctcgactatc
     6361 acatgcagat cctcgggctc gtagtagtcg agcttcccca agaatccgaa cctgtcgcga
     6421 agcggcgaag tgagcagccc cgtgcgggtg gttgctccta ccagagtgaa tgcgggcaag
     6481 tcgatcctta tggaacgcgc agctggtccc ttgccgatga tgatgtcgat cgcgcgatcc
     6541 tccatagctg ggtacaggat ctcctcgacc ggcctggcca aacggtgtat ctcatcgatg
     6601 aagagaacat caccttcctg cagattggtg aggatggcag caaggtcccc ggcccgctcg
     6661 agggcgggcc ccgatgtgac ccttagagtg gcattcaact cgttcgcgat gatgttggcg
     6721 agtgtcgtct tgccgagccc gggactccct gcgaagagca ggtggtcggg tgactcgccc
     6781 ctattccggg cggcttcgag gatgatcgag aggtgctctt tggtcggttc ctgccccacg
     6841 aactcggtga gaagtcgcgg gcggagactc tcttcgaagt cagactcctc gggtgagacg
     6901 tctaccaaca gatcggaact catgccctgg ccagtccctt gagtgcctgc ttgaccatct
     6961 gttcgacctg caggtcggag tcgtccaacc ccgcgagcgc ggagcggacc tccgatgttt
     7021 cgtatccaag actggcgagg gcctctcgcg cttcgatcat gcgtggatcg ctcgcttcgt
     7081 cgctctcggg ggcccccagc ctccccttca gctctacgat catccgctga gcggtgcgtt
     7141 tgccgatccc gggcacggtc acaagaagct ccacatcgtc cgaagcgatc gctccgcgaa
     7201 gagcggtctc atcgagtgac ccgaggatcg tcagagcgat cttggccccc agcccgttaa
     7261 ccgagagcag caccttgaag agttcgcgct catccgcgga agcgaaccca tacagagaga
     7321 tcgagtcctc ccgcacgatg gtctggaccg cgagacgcac gacctttccc acgcgcaatg
     7381 tcgagatcgt tcttgtcgaa caggcgacct ggtagccgac cccgttgaca tcgacctcga
     7441 cagtgccatc gctcagatcg gtcacagaac ccttgagaga cgaaatcatg tccttgttcc
     7501 tatcactcgt cgcacgcgat cgtggctcag gtgggcgatc gcgacagcca cggcgtcgga
     7561 cgcgtcggga cccgctgcgg tcgcggtatc acctgtgatc ttgctgacca tgaaagccac
     7621 ctgttccttc gacgcactcc ccgttccgac aactgcatgt ttgacctcca tcgggctgta
     7681 ctcgaagacc ggcatccctc gttgggcggc aacgacaaga gcgacaccgc ttgcttggat
     7741 cgccccaacc gcgctcatgc ggttcgcatt gaggaatact cgctcgaccg ccatcgaatc
     7801 agcatctgag ccatcggcga cttcgcagat gtcccgccac aagctcgcca ggcgctcgga
     7861 tgcctgccca tctggtgacg tcgtgaaggt gcctgaccgc aggcagacga tcctgcccgc
     7921 gtcctcctcg acaaccgcat aaccgagacg ggtcagccct ggatcgatgc ccagaactcg
     7981 cacacctcga ctctggctca tcatccacct cgcgatcaat caatcgaaca tgtgtttgat
     8041 tgatgatgat aggtcgcgct gaggcaaagc gagtggattt agggccttat ctagctacgg
     8101 gaaaggtcga ggaagagaag attcatctga gtcgtcaacg cgtcgtcgag gtctgttgga
     8161 tcaagcagac aacggtcgac gaggatctcg cctagtttcc gaccactctc ggcctgatca
     8221 cccagtgccg tccaaagttc gtccttggtg atgaggtt
//
LOCUS       DATDOZ010000198         3666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3558914, whole genome shotgun sequence.
ACCESSION   DATDOZ010000198 DATDOZ010000000
VERSION     DATDOZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558914"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VI541_03970"
     CDS             <1..672
                     /locus_tag="VI541_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HLF70094.1"
                     /translation="KVIKDANLEIKVGKGEFQRRFTAAQNLAESFGGFVAESTITHQK
                     DELTSGSLTIRVPGDRFEQAVTALKKLGKVTNESQSGQDVTSQVVDLEARLRHAKAQE
                     TVFLRLMDQSKSISDIIAVQQQLSNVQLEIEQLQGQLNAITDLVSMATIRMSLFEPGA
                     KAPGTPRGIAKAWQEALDGAVNVVAGVIVVAGWLAPIAVVAIIVWLIVRRRKGRADKA
                     GAESG"
     gene            complement(664..734)
                     /locus_tag="VI541_03975"
     tRNA            complement(664..734)
                     /locus_tag="VI541_03975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(700..702),aa:Gly,seq:ccc)
     gene            803..1372
                     /gene="dcd"
                     /locus_tag="VI541_03980"
     CDS             803..1372
                     /gene="dcd"
                     /locus_tag="VI541_03980"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLF70095.1"
                     /translation="MILSDRDIRTEVDAGRLVIKPFEPGMVQPSSIDVRLDNQFRVFH
                     NHRYRVIDVKTRMDELTELVEVREEEPFILHPGEFVLGSTREWVEFPNDIAGRLEGKS
                     SLGRLGLVTHSTAGFVDPCFEGHLTLELSNLANLPITLYPGMKIGQIAFFRLSSPAEV
                     GYGSGELRSKYKGQRGPTPSKYWENFDQS"
     gene            complement(1392..1781)
                     /locus_tag="VI541_03985"
     CDS             complement(1392..1781)
                     /locus_tag="VI541_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLF70096.1"
                     /translation="MNENGQLIARLYGAFESSDLDTIRESLDEDVILHQAGRGPLSGV
                     HEGRDEVLGVLGSLIALSEGTFHSKVHDILANDEHVVVLSENTASARGKTLQERGVEI
                     FHVTDGKVTEAFFTAFDLYEMDEFFTA"
     gene            complement(1819..2346)
                     /locus_tag="VI541_03990"
     CDS             complement(1819..2346)
                     /locus_tag="VI541_03990"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF70097.1"
                     /translation="MITSIAAFIRWFEGVNRRAMRDIGGLPQEAEGWRPTLGEGEKAW
                     SIGEIVGHMSVSRLFFLSTFMGPEWSGAALDMDTTARASWTDALQRSCEIVTETLATA
                     PDEYITRKIPMLDGSGREMSGWRPLMLMAEHDIHHRSQIDTYAGMNGWEVSQIFGRSA
                     EQVGLGAEQATQRQD"
     gene            complement(2362..>3666)
                     /locus_tag="VI541_03995"
     CDS             complement(2362..>3666)
                     /locus_tag="VI541_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLF70098.1"
                     /translation="PAWNTGKPLSPKLLIMDLRDHLFEQGPGLLREKRDREGPPLPKW
                     TPEKLRKFIVEGPPAKMRTTMLDMRDKMLGKPIGENKPLNPDVIEDEVVWSCTTCGWC
                     VHNCPVDIEHIDTIIDMRRNLVMMESRFPKEMQTALTNLENSGNPWGQSQSGRVEWTE
                     GLDVPVLGEAGSADQYEVIYWVGCAGAFDDRNKKVVRSFAKLMKAAEVSFAILGTNEQ
                     CNGDPARRMGHEYLFQTMAQANIETLDGFGVKHIVTACPHCFNTLRNEYPQFGGNYQV
                     RHHSEFLAQLVEQGRLRIQEDYLKTVAYHDPCYTARHNDIIEAPRKILAGAGAQTREL
                     HRHGKQTFCCGAGGGRMWMEERVGKKVNIDRTDEALASGVDVIGVGCPFCHIMLDDGV
                     KERGAQERTSVQDLAQILEAVVIPAGAKLLPLANGNGQPVRD"
BASE COUNT          669 a         1151 c         1094 g          752 t
ORIGIN      
        1 aaggtcatca aggacgcgaa cctcgagatc aaggttggca agggggagtt ccagcgacgg
       61 ttcaccgcgg cacagaacct tgctgagtcc ttcggcgggt tcgtcgcaga gtcgaccatc
      121 acccaccaga aagatgaact cacgtcgggc tccctaacga tccgcgttcc gggcgatcgc
      181 ttcgagcagg cggtgactgc cctcaagaag ctgggcaaag tcacgaacga gtctcagtcc
      241 ggccaggacg tcacctcaca ggtcgtcgac ctcgaagctc gtcttcgtca cgccaaggct
      301 caggagaccg tcttcctgcg gctcatggat cagtccaaat cgatctcaga catcatcgcg
      361 gtccagcagc agctctcgaa tgtgcagctc gagatcgaac agctgcaggg tcaactgaac
      421 gcgatcaccg acctcgtctc gatggccacg atccgcatgt cgctcttcga gccgggcgca
      481 aaggcccccg gcaccccacg aggcatcgcc aaggcatggc aagaagcgct cgatggcgca
      541 gtcaatgtgg tagctggggt catcgtcgtg gccgggtggc ttgcgccgat agcggtcgtc
      601 gcgatcatcg tctggctcat cgtgcggcgg cgcaaaggcc gggccgacaa ggcgggggcc
      661 gagagcgggt gacgagaatc ggactcgcgc ctgaagcttg ggaagcttcc gttctaccat
      721 tgaactacac ccgcgaaact cacatatctt aaccgcggcc ccggtcacga acccaaaggg
      781 gccgcggtag gattccactc cgatgatcct ctctgaccgt gacatccgaa ccgaggtcga
      841 cgctggtcgc ctcgttatca agccattcga gccgggcatg gtccagccgt catcgatcga
      901 cgtacggctc gacaaccagt tccgcgtctt tcacaaccac cgctatcggg tcatcgatgt
      961 gaagacacgt atggatgagc tgaccgagct cgtcgaggtg cgcgaagagg agccattcat
     1021 ccttcatcca ggcgagttcg ttctaggttc gacccgcgag tgggtggagt ttcccaacga
     1081 catcgccgga cgcctcgaag gaaagtcgtc gctcggccgt ttgggcctgg tgactcactc
     1141 gacagccggg ttcgtcgacc catgtttcga gggtcacctc accctcgagc tgtcgaacct
     1201 cgccaacctg ccgatcaccc tgtatccggg gatgaagatc ggtcagatcg cgttcttccg
     1261 actctcctca ccagccgagg ttggatacgg gtcgggtgag ctgcggagca agtacaaggg
     1321 ccagcggggg ccgacgccat cgaagtactg ggagaacttc gaccagagct gacttcagta
     1381 ccgggctcga ctcatgccgt gaagaactcg tccatctcgt agagatcgaa cgcggtgaag
     1441 aaggcttcag tcaccttgcc gtccgtgacg tggaatatct cgacccctcg ctcttgcagg
     1501 gtcttgccgc gtgcggatgc ggtgttctcg ctcaacacga ccacgtgctc gtcgttcgcg
     1561 aggatgtcgt ggaccttgga gtggaaggta ccctcgctca gtgcgatgag agagccgaga
     1621 acccccaaca cttcgtcgcg accttcatgt acgccgctta gggggccgcg tcctgcctgg
     1681 tgcaggatga cgtcttcgtc gagtgactcg cggatggtgt cgaggtccga gctttcgaag
     1741 gccccataga ggcgagcgat cagctgtccg ttctcattca tgttgtgctc ctcgtcagtc
     1801 ggcatcggtt cgccaactct aatcctgtcg ctgcgtcgct tgctcggccc ccaggccgac
     1861 ctgctccgca gatcgtccga agatctgcga aacctcccac ccgttcatcc cagcgtaagt
     1921 gtcgatctgt gagcggtggt ggatgtcgtg ttcggccatc agcatcagag ggcgccagcc
     1981 ggacatctca cgtccagatc catcaagcat ggggatcttt ctggtgatgt actcatcggg
     2041 tgcggtcgca agggtctcgg tcacgatctc gcagcttctc tggagagcat cggtccatga
     2101 cgcgcgggcg gtcgtgtcca tatccagtgc cgcgcccgac cactcgggcc ccatgaatgt
     2161 ggacaggaag aagagccgcg atacgctcat gtgacctacg atctctccga tgctccacgc
     2221 cttctcgccc tcgccaaggg ttgggcgcca accctctgcc tcctgaggaa gtccacctat
     2281 gtcccgcatc gctcgtcggt tgactccttc gaaccaacgg atgaaggccg cgatgctagt
     2341 gatcatcgac tcatcgccaa ctcagtcgcg caccggctgg ccattgccgt tggcgagtgg
     2401 aaggagcttg gcccccgcgg ggatgacgac cgcttcgagt atctgagcga ggtcctgaac
     2461 cgaggtccgc tcttgtgccc cgcgctcttt caccccgtcg tcgagcatga tgtggcagaa
     2521 gggacagccc acaccgatga catcgactcc gcttgcgagc gcctcgtccg tccggtcgat
     2581 gttgaccttc ttgccgaccc gctcctccat ccacatacgg cccccacccg cgccacagca
     2641 gaaggtctgc ttgccgtggc gatgaagctc cctcgtctgg gcccccgctc ccgcgaggat
     2701 cttgcgcgga gcttcgatga tgtcgttgtg gcgtgcggtg tagcaggggt cgtggtaggc
     2761 gacggtcttg aggtagtcct cctgtatccg taggcgcccc tgttcgacca actgcgcgag
     2821 gaactcgctg tgatggcgca cctggtagtt accgccgaac tgcgggtact cgttgcgcag
     2881 ggtgttgaag cagtgcgggc acgccgtgac gatgtgcttg acgccgaacc catctagcgt
     2941 ctcgatgttc gcctgggcca tcgtctggaa gagatactca tgccccatac gtcgcgcggg
     3001 gtccccgttg cactgttcgt tggtcccgag gatcgcgaac gagacttcag ctgccttcat
     3061 gagtttggcg aacgaacgca cgaccttctt gttgcggtcg tcgaatgccc cggcgcaccc
     3121 tacccagtag atgacttcgt actgatcagc cgatcccgcc tcgccaagca caggaacatc
     3181 gagcccttcg gtccactcga cccgcccgct ctgcgactga ccccagggat tccctgagtt
     3241 ctcgaggttg gtgagagcgg tctgcatctc cttggggaat cgcgactcca tcatcacgag
     3301 gtttcggcgc atgtcgatga tcgtgtcgat gtgctcgatg tccaccggac agttgtgaac
     3361 acaccatccg catgtcgtgc atgaccagac gacctcatct tcgatgacat cagggttgag
     3421 tggcttgttc tcaccgatgg gcttgcccag catcttgtcg cgcatgtcga gcatcgtcgt
     3481 gcgcatcttt gcaggcggcc cctcgacgat gaacttgcgc aacttctcgg gggtccactt
     3541 agggagtggg ggcccctctc ggtcgcgttt ctcgcgcaga agacccggtc cctgttcgaa
     3601 gaggtgatct cgaagatcca tgatcagtag tttgggcgag agcggcttgc cggtgttcca
     3661 ggccgg
//
LOCUS       DATDOZ010000199         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2786568, whole genome shotgun sequence.
ACCESSION   DATDOZ010000199 DATDOZ010000000
VERSION     DATDOZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2786568"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..401)
                     /locus_tag="VI541_04000"
     CDS             complement(147..401)
                     /locus_tag="VI541_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HLF70099.1"
                     /translation="MRSLLFWIVVGGVAGWLASIVTGTGKRMGCLLNILAGVVGAVIG
                     GWVFQKLDLVAPSGHFVGSIVVAFVGAVIFLGVLRLFGKR"
     gene            complement(529..1530)
                     /locus_tag="VI541_04005"
     CDS             complement(529..1530)
                     /locus_tag="VI541_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLF70100.1"
                     /translation="MTQAQRKPLERCRAITASSRRCRNLAVDGRGFCRVHAGPAKRLI
                     APAADRTPPEAIAHDRVHELIDFARRRMLGDYQVDDFGFDPDLVNSILQPIAMLLYEK
                     YWRVTTIGIENIPSHGSALIVANHSGTLPWDAVMMQTGIRAEHPAKRDVRLIGADLVW
                     EFPFISHLARKTGNTVAIEDDTLRLLGSGELVGVFPEGYKGVGKPWAQRYKLQRFGRG
                     GFVQVALKARVPIIPTAIVGAEEIYPMIANFKPLARMLGFPYFPITPLFPLLGPLGAI
                     PLPSKWMIEFGTPIATDVYEEDAHQDPMLVFDLTDRVRDIVQQMIDNNLAARKGVFG"
     gene            complement(1527..2588)
                     /locus_tag="VI541_04010"
     CDS             complement(1527..2588)
                     /locus_tag="VI541_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF70101.1"
                     /translation="MGKRVLITGVSKFLGASLARRLQDDPEVDYVAGVDLRPPPFELP
                     GVEYLRADIRTPLIQKIIATAQADTVVHCDLTSSPVRVGGRSAQKEQNVIGTMHLLGA
                     CQRSEIVRKVVVRSSTAVYGIDSSDPSILSEDRLHAAPPVHGYSKDVFDAETSARDFG
                     RRRPEVTLTILRMANVVGPRAETNMTQFFSLPVIPTALGFDPRLQLLHEDDAVEVLRR
                     SVVEDHPGVYNVAADGVMYLSQAIRIARRIPMPILAPLAVMIGDTVRRTGVIDFSTDQ
                     INLIVHGRVVDTTRLKDIFGYAPAYSTLEAFRDFVESTSRAEGGLTGWEKEIYAFITR
                     TGARAPGLRRVENLKEGLR"
     gene            2666..>3199
                     /locus_tag="VI541_04015"
     CDS             2666..>3199
                     /locus_tag="VI541_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF70102.1"
                     /translation="MSIASRILYLEPLADLRSDEWLVRAAKSGNSEAAATLFRRYHGR
                     VLAFISHMTGGRSNSEDITQETFIRALRGLDGFDGRRFEAWLLRIARNLVIDEARRDP
                     SQPTPVPPEELAAIEPKQGRSGGVWDLYSANLATTAVHEALAKLPIRQRTVIVLRDIE
                     GRSRDEIAEILGTNPHAI"
BASE COUNT          640 a          996 c          973 g          590 t
ORIGIN      
        1 cctctacagc gcctatatcg acccttacgt cgaggccgag gccaaagagg tcgggatcgc
       61 ggtttgcgtt gccaagtcgg aaggtttgga gactttggaa agaaggatct cggaactctg
      121 tcttcagttc ggcaccgatt aaaggttcag cgcttcccga acagcctcaa gaccccgagg
      181 aatatcaccg ctccaacgaa ggcgacaacg atgctaccca cgaagtgccc gctcggcgcg
      241 accagatcta gtttctgaaa gacccaaccg ccgatgaccg caccaaccac accggcgagg
      301 atgttgagca ggcaacccat gcgtttgccg gtcccggtaa cgatacttgc gagccatccg
      361 gccaccccac ccaccacgat ccagaacaac agagacctca tccgtgcccc ccatcaaacg
      421 gtatcctcat cgagtgcgcg ccagaacctt gcttgcagtt cttcttctgc tcgcggccat
      481 agcagccgcg gggatcactt cggtgatact catgtcgagg atcccgcact agccgaagac
      541 acccttgcgt gcggccagat tgttatcgat catctgctga acgatatcgc gcaccctatc
      601 ggtcaggtcg aagacgagca tcggatcctg atgagcatct tcctcgtaca cgtccgtcgc
      661 gatgggtgta ccgaactcga tcatccattt cgaaggaagc gggatcgctc ccagggggcc
      721 gagcagcggg aagagcggtg tgatggggaa gtaggggaac ccgagcatgc gggctaacgg
      781 tttgaagttt gcgatcatcg gatagatctc ctcggccccc acgatcgcgg ttgggatgat
      841 cggcacgcgc gccttgagag ctacctgtac gaatcccccc cggccgaacc gctggagctt
      901 gtacctctga gcccagggct tgcccacacc cttgtaccct tccgggaaga caccgaccaa
      961 ctcgccggac ccgagtagac gcagtgtgtc gtcctcgatg gcgaccgtgt tgccggtctt
     1021 gcgggcgagg tgcgagatga aggggaactc ccagacaagg tcggcgccga tgagtcgcac
     1081 gtcgcgcttc gccggatgct ccgctctgat gcccgtctgc atcatcaccg catcccacgg
     1141 aagcgtcccc gagtggttcg ccacgatgag cgccgagccg tgcgatggga tgttctcgat
     1201 accgatcgtc gttacacgcc aatacttctc atacagcagc atggcgatcg gctgcaggat
     1261 gctgttgacg aggtcgggat cgaagccgaa gtcatcgacc tggtaatcgc caagcatccg
     1321 tctgcgggcg aagtcgatca gttcgtgaac tcgatcgtgc gcgatcgcct ctggtggagt
     1381 gcgatccgcg gcaggtgcga tcagtcgctt ggccggtccc gcatgtacgc gacagaaccc
     1441 gcgcccatca actgccaggt tgcgacagcg acggctggag gcggttatcg ccctgcatcg
     1501 ctccaggggt ttgcgctgcg cttgcgtcat cgcaggccct ccttcaggtt ctcgaccctt
     1561 cgcaagccgg gggcccgggc ccccgtacgc gtgatgaagg cgtagatctc tttctcccac
     1621 ccggtgagcc cgccttcggc ccgagaggtt gactcgacga agtctcggaa agcttcgaga
     1681 gtcgaatacg ccggagcgta gccgaagatg tctttcagtc gcgtcgtatc aaccactcgc
     1741 ccgtgaacga tgaggttgat ctgatcggtc gagaagtcga tgacgccagt cctgcgaacc
     1801 gtgtcgccga tcatgaccgc gagcggagcg aggatgggca tgggtatgcg cctggcgatg
     1861 cggatcgcct gcgacagata catcacgcca tccgccgcga cgttgtatac gcctggatgg
     1921 tcttcgacga cgcttcgtcg caacacctcg acagcatcgt cttcatgcag gagttgcaac
     1981 cgcgggtcga aacccagcgc ggtgggaatc accggcaacg agaagaactg agtcatgttc
     2041 gtctctgccc taggccccac aacattggcc atgcgcaaga tcgttagtgt cacctcgggc
     2101 cttcgacgtc cgaagtcgcg ggccgaggtc tcggcatcga aaacgtcctt cgagtacccg
     2161 tgaacgggcg gcgccgcgtg cagtcggtcc tcactgagga tggagggatc cgacgaatcg
     2221 atcccgtaca cggcggtcga tgagcgcacg acgactttac ggacgatctc cgaacgctga
     2281 catgccccga gcagatgcat ggtgccaatc acgttctgtt ccttctgggc cgaccgtccc
     2341 ccaacacgca cggggctcga tgtcaggtcg cagtggacga cggtgtcggc ctgggcggtc
     2401 gcgatgatct tttggatgag gggtgtgcgg atgtcagccc tcaggtactc aacccctggt
     2461 aactcgaacg gcgggggccg aaggtcgact ccggctacgt aatcgacctc ggggtcgtcc
     2521 tgcaatcgac gggcgaggct cgctccaagg aacttagaga ccccggtgat aagtactcgc
     2581 ttgcccaccg gccaaggcta tagcagaacg agcgagcagg acatcggtac atcggtgtgg
     2641 aagtattccc gcgggggtca acgagatgtc gatcgcaagc aggatccttt acctggagcc
     2701 actggccgac ctgaggtcgg atgaatggct cgtgcgcgcc gccaaatcgg gaaactccga
     2761 ggcggcggcg accctcttcc gccggtatca cggccgtgtc cttgccttca tctcgcacat
     2821 gaccggtggt cgatctaact ccgaggacat cacccaggag accttcatca gggcgctgcg
     2881 ggggcttgat ggattcgatg gaagacggtt cgaggcctgg ctacttcgga tagcccgcaa
     2941 cctcgtcatc gatgaggccc gcagggaccc ctcgcagcca acaccagtac ctcccgagga
     3001 actcgctgcg atcgaaccca agcaggggcg ttcgggggga gtctgggacc tctactcggc
     3061 caacctggcc acgacggcgg ttcacgaggc cctggccaag ttaccgatcc gacagcggac
     3121 ggtgatcgtg ctgcgcgaca tagagggccg ctcgcgagac gagatcgccg agatactggg
     3181 gaccaacccg cacgcgatc
//
LOCUS       DATDOZ010000200         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2519878, whole genome shotgun sequence.
ACCESSION   DATDOZ010000200 DATDOZ010000000
VERSION     DATDOZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519878"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..560)
                     /locus_tag="VI541_04020"
     CDS             complement(42..560)
                     /locus_tag="VI541_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HLF70103.1"
                     /translation="MGKHSAPQRARFYRSLAAYVARWLAVLALLGGVAYGLIRLLPRE
                     VPSLSASRGPSASPSPVPSIEPGEPGVVDSGSPSPSESAGTVQVLDGAGKLANVDAAI
                     ATLNRLGYRVLGPEATSRHYATTTVFYQPDERQLADAVAEAFHGAQVVAAPSSLDPSI
                     PVTLVIGDDFQV"
     gene            complement(564..845)
                     /locus_tag="VI541_04025"
     CDS             complement(564..845)
                     /locus_tag="VI541_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HLF70104.1"
                     /translation="MREEPAATFGDRERKILEFERYWWKYAGAKERAIREQFDLSATR
                     YYQLLNEIIDLADALAYDPILVKRLQRLRAFRQKQRMARRLGIRITDNA"
     gene            complement(921..2168)
                     /locus_tag="VI541_04030"
     CDS             complement(921..2168)
                     /locus_tag="VI541_04030"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HLF70105.1"
                     /translation="MESMNPWEAALEYLDEAAELMGLDEGTHKILRAPKRALMVSVPV
                     RMDDASIEVFQGYRVHHDVTRGPAKGGIRYHPDVSLDEVKALAMWMTWKCAVVNIPYG
                     GAKGGIIVDPRALSQGELERMTRRYTSELIPLIGPERDIPAPDVNTDEQIMAWMMDTY
                     SMNVGYSVPGVVTGKPLSVGGSFGRGGSTSRGVMYQIFSALKTLGMGIEDVSVAVQGF
                     GKVGGGVARMLHDQGCRIVAVSDVVGGVMNERGLNPEAIDRFKHGAGSVAEFPEGDRL
                     TNEELLELDVDVLVPAALEGVINESNADLLRARIIAEGANGPTTPKADRILSERGVFI
                     IPDILANAGGVTVSYFEWVQDLQAYFWSEDEVNHKMRQIMEASFLDVYGMHKAKGYTM
                     RQAATILAVSRVAEAHRVRGLYP"
     gene            complement(2209..2282)
                     /locus_tag="VI541_04035"
     tRNA            complement(2209..2282)
                     /locus_tag="VI541_04035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2246..2248),aa:Thr,seq:tgt)
     gene            complement(2260..>2461)
                     /locus_tag="VI541_04040"
     CDS             complement(2260..>2461)
                     /locus_tag="VI541_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70106.1"
                     /translation="HHIYVHEAGYTVRGSPPKVIIERPGREPLRVGPPASMNDNNGPH
                     RGPFPLLMDNVALWDRWRGAAR"
BASE COUNT          463 a          813 c          732 g          453 t
ORIGIN      
        1 acgatccaca tcgagcagcg gtcggagatg agtcggatgg cttagacctg aaagtcgtcg
       61 ccgatcacaa gagtcactgg gatcgagggg tcgaggctgc tcggtgcggc gaccacctgc
      121 gctccatgaa aggcctcggc aaccgcatcg gccaactgtc gctcgtcggg ctgatagaag
      181 acggtggtgg tcgcatagtg acggctcgtc gcctcgggac caagcacccg gtagcccaga
      241 cggttgaggg ttgcgatggc cgcatcgacg ttcgccagct tgcccgcccc atcgagaacc
      301 tggacagtgc ccgccgactc agaaggcgag ggcgagccgc tgtcgacgac acctggttcg
      361 cccggctcga tggaagggac cggcgacggc gacgcgctcg gcccccgcga ggctgagaga
      421 ctgggcacct ctcggggcaa gagccggatc agaccgtacg caacgccacc caggagggcc
      481 aggactgcga gccagcgagc aacgtaggct gcgagagacc tatagaagcg ggcgcgctgc
      541 ggcgccgagt gctttcccat ccatcaagcg ttgtcagtta ttcggatccc caaccggcga
      601 gccatcctct gcttctggcg gaatgccctg agcctctgca acctcttgac gagtatcggg
      661 tcataggcaa gagcatcggc gagatcgatg atctcattga gcaattggta gtaacgcgtc
      721 gctgagaggt cgaactgctc gcggatcgcg cgttccttgg cccccgcgta cttccaccag
      781 taccgctcga actccaagat cttgcgttct cggtccccga aggttgccgc tggttcttcg
      841 cgcaaggctc agcctccctc gaatcgcatg gttgtgatga gcttcacctt aggcagagac
      901 gagcggcatt gcaaggaccg ctagggatac agacccctca cgcgatgggc ctcggccaca
      961 cgggagactg ccaggatcgt cgcagcttga cgcatcgtgt accccttggc cttgtgcatc
     1021 ccatagacat cgaggaatga ggcctccatg atctgtctca tcttgtggtt cacctcatcc
     1081 tcactccaga agtaggcctg cagatcctga acccactcga aataggaaac agtcacgccc
     1141 ccggcgttgg cgaggatgtc ggggatgatg aagaccccgc gctcgctcag tatccggtcc
     1201 gccttcgggg tcgtcggtcc gttagcgccc tccgcgatta tgcgggcgcg cagcaggtcc
     1261 gcgttgctct cgttgatcac gccctcgagg gccgccggaa ccagaacatc gacatcaagt
     1321 tcaagtagct cctcgttcgt taggcggtcg ccctcgggga actcggcgac cgaccccgct
     1381 ccgtgcttga agcggtcgat cgcctcaggg ttcaagcccc tctcattcat cactccgccc
     1441 actacgtccg agacggccac gatccggcaa ccctgatcgt ggagcatccg agcgacccct
     1501 ccaccgacct tgccgaaccc ctggacggcg accgagacat cctcgatgcc catacccagc
     1561 gtcttgagcg cggagaagat ctggtacatc acgcccctcg acgtcgagcc accacgaccg
     1621 aacgagccgc caaccgacaa tggcttaccg gtcaccacac ccggcaccga gtaaccgacg
     1681 ttcatcgagt aggtgtccat catccaggcc atgatctgtt cgtcggtgtt gacgtcgggg
     1741 gccgggatgt ctcgctcggg gccgatgagt gggatcaact cggatgtgta tcgacgggtc
     1801 atgcgttcca gctcgccctg ggacagggcc ctcggatcga cgatgatccc gcccttggcc
     1861 cccccatacg ggatgttcac gaccgcgcac ttccatgtca tccacatggc gagcgccttc
     1921 acctcgtcga ggctgacatc agggtgatag cggatgcctc ctttggcggg cccccgcgtc
     1981 acgtcgtgat gaacgcgata accctggaag acctcgatcg aagcatcatc catccgcacg
     2041 ggaaccgaca ccatgagggc ccgcttgggg gccctgagga tcttgtgcgt accctcgtcg
     2101 agccccatca actcggccgc ctcgtcgaga tactcgagcg cggcctccca tgggttcatc
     2161 gattccatct gaccctccct cggcccgtct tcgaaggact cacctttgag ctggcggtcg
     2221 gattcgaacc gacgacctag cgattacaag tcgcttgcgc taccgggctg cgccacgcca
     2281 gcgatcccac agcgccacat tatccatgag aagtggaaac ggcccccgat gggggccgtt
     2341 gttatcgttc atcgaggcgg ggggccccac cctcaagggt tctcgaccag gtctctcgat
     2401 gatcaccttc ggcggtgagc cgcggaccgt gtagcccgcc tcgtgtacat agatgtggtg
     2461 g
//
LOCUS       DATDOZ010000201         3327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1489529, whole genome shotgun sequence.
ACCESSION   DATDOZ010000201 DATDOZ010000000
VERSION     DATDOZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489529"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VI541_04045"
     CDS             complement(<1..903)
                     /locus_tag="VI541_04045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HLF70107.1"
                     /translation="MVTRMNTVTLPAFRELVPPHNLEAEESVLGACLLSREAIAAALE
                     VLAADDFYKPAHVEIFNAILELYGRGEPIDGVTVTEELRRRGSLDNVGGRPYIFTLIE
                     SVPTPGSAAYYAQIVEEHALLRRLIDASHGISEIAYAIPEDVSGAIDQSEDLIYKVSQ
                     RRQAQSFSALKDLLTENMEMVERLYEQGSSITGLPTGFTELDNITAGLQPSNLIIIAA
                     RPAMGKSALAVSMAQSIAIREKVPVVIFSLEMSKTEVVQRLMCAEARVDSNRLRRGAL
                     QDSDWPKLSHALGRLAEAPIFIDDT"
     gene            complement(1181..1792)
                     /gene="rplI"
                     /locus_tag="VI541_04050"
     CDS             complement(1181..1792)
                     /gene="rplI"
                     /locus_tag="VI541_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HLF70108.1"
                     /translation="MKIILNHDVEKLGRKGDVVEVAAGYARNFLLPRNLAIAATRGAL
                     RQAEHLIKAREVLDTKQRAVLGELAEKISSQRLNIAVRVGDEGQMFGSVTTAEIADLL
                     AKASGESIDRRKVGVETTIKSLGVHTYTVHLGLDVVASGTIEVVPDATSPKVFVPKAE
                     EGAEPTDVAALSDEASSDGSSTDETSADEVPSDEADSDESAAE"
     gene            complement(1789..1992)
                     /gene="rpsR"
                     /locus_tag="VI541_04055"
                     /pseudo
     CDS             complement(1789..1992)
                     /gene="rpsR"
                     /locus_tag="VI541_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(2060..2482)
                     /gene="ssb"
                     /locus_tag="VI541_04060"
     CDS             complement(2060..2482)
                     /gene="ssb"
                     /locus_tag="VI541_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLF70109.1"
                     /translation="MANNITVVGNLTREPELKYTPSGAAVAKFGIAVNRFYTSRTGEK
                     VEQTDFFNVNAWRQLAENVAETLTVGTRVIVSGRLQSRSWETEDGQKRTVVEIEADEI
                     GPSLRWATATIAKTTRGGPGDWSDNSTPAETEEVPAGT"
     gene            complement(2513..2794)
                     /gene="rpsF"
                     /locus_tag="VI541_04065"
     CDS             complement(2513..2794)
                     /gene="rpsF"
                     /locus_tag="VI541_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLF70110.1"
                     /translation="MRNYDAVVIVDPRLDEAAIQQAVDRFTKVIETRGEITKLDVWGR
                     RRLAYEIDHLTDGHYVVANFKADPTLIRELDRLFEIGEEYVRAKIVRLP"
BASE COUNT          634 a          998 c          984 g          711 t
ORIGIN      
        1 ggtgtcgtcg atgaagatgg gggcctcggc caggcggcca agagcatgcg agagctttgg
       61 ccagtctgaa tcctgcagag cgccccttcg cagacggttc gagtcgaccc gcgcctcggc
      121 gcacatcaga cgctggacga cctctgtctt ggacatctcg agcgagaaga tgacaactgg
      181 aaccttctcg cgaatcgcga tgctctgagc catcgagacc gccagggcgg actttcccat
      241 cgcgggccgt gcggcgatga tgatcaggtt cgagggctga aggccagcgg tgatgttgtc
      301 gagctcagtg aagcctgtgg gaagaccggt gatcgaggag ccctgctcgt agagcctctc
      361 gaccatctcc atgttctcgg tgagcaggtc cttgagggcc gagaagctct gggcctgtcg
      421 gcgctgggag accttataga tgaggtcctc ggattgatcg atcgcccctg acacatcttc
      481 ggggatggcg taggcgatct cagagatgcc gtgagatgca tcgatgagcc ggcgaaggag
      541 agcatgctcc tcgacgatct gcgcgtagta ggccgcagag cctggggtgg ggacactctc
      601 gatgagtgtg aatatgtagg gacggccccc gacgttgtcg agactgcctc tgcgccgtaa
      661 ctcttcggtg acagtgacgc catcgatcgg ctcgccgcgg ccatacaact cgaggatcgc
      721 attgaatatc tcgacatgtg ctggcttgta gaagtcatcg gcggccagca cctcgagcgc
      781 cgccgcgatg gcctcgcggg agagcaggca cgctcccaag acagactcct cggcctcgag
      841 attgtgcggc ggaaccagct cgcgaaacgc cggaagagtg actgtgttca tcctggtaac
      901 catcgatggc ctctcacgaa cagatgttcg tataagtgta agactcggct ctgacgctca
      961 ggtggcaatc ccgctccaag cgcgccgaat caggagttac tttctgtgat tccccctgat
     1021 ctccggtgtt gtccacctat tttccaccgg aaatacaccg tcttttccac aaaatacaac
     1081 cgcccgtttc cgggcgtcgg cgcaatatat tgagccgcta atagcacgtc aatagggcgc
     1141 gctcaaatag tgggttgaac gggaaagaaa gcttcgcgag ttactccgcc gccgactcgt
     1201 ccgagtcggc ttcgtcgcta ggcacttcgt cggcagacgt ttcgtcagta gacgatccgt
     1261 ccgaagacgc ttcgtcggaa agggcggcaa catcggttgg ctcggccccc tcttcggctt
     1321 tcggcacgaa gaccttcgga gatgtcgcat cgggaacgac ctcgatggtt ccgctggcga
     1381 cgacatcgag ccccagatgc acggtgtatg tgtgaacacc caaagacttg atcgtcgtct
     1441 caacaccaac cttgcggcga tcgatggact caccggacgc cttggcgaga agatctgcga
     1501 tctcggctgt ggtgaccgac ccgaacatct ggccctcatc ccccactcgc acggcgatgt
     1561 tgagtctctg cgaagagatc ttctcggcaa gttcgccaag aacggctcgc tgtttcgtgt
     1621 cgagcacctc gcgggccttg atcagatgct cggcctggcg cagggccccc ctcgtcgcgg
     1681 ctatggcgag gttgcgcggg agaaggaagt ttcgtgcata acccgcggcg acctcgacaa
     1741 cgtcgccctt gcggccaagt ttctcgacat cgtggttgag gatgatcttc atcggaccac
     1801 gtatgggagt agagccactt ctctggcgtt cttgaccgca gtggcgacct catgctggtg
     1861 ctggacgcag gttccggtga cacgccgcgc acggatcttg cccttgtcgg acacgaactt
     1921 gcgcatgaca gagacgtcct tgaagtcgac gtagccgatc ttctctttgc agaagtaaca
     1981 gaccttcttg cgctgaggtc ggtcacgctt cttgtctttt ccccggtctt tgctccggct
     2041 ctccgttgcc cgggatgcct tacgtgccag cgggtacctc ctcagtttca gccggtgtgg
     2101 agttgtctga ccagtcgcca ggccccccac gggtggtctt tgcgatggtc gcggtggccc
     2161 agcgtagcga tggaccaatc tcgtcggcct ctatctcgac aaccgtgcgc ttctgaccat
     2221 cttctgtctc ccagctccgt gactgaagcc gaccgctgac gatcactcgt gtccccaccg
     2281 tgagcgtttc ggcgacgttc tcggccagct gacgccatgc gttaacgttg aagaaatcgg
     2341 tctgctcgac cttttcgcca gttcgtgacg tgtagaacct gttcacggcg atcccgaact
     2401 tcgcaaccgc cgcccccgag ggcgtgtact tcaactcggg ctccctggtg aggttcccca
     2461 ccacagttat gttgttagcc atctgcctac ctccttcggg gctctgcccc actcacggga
     2521 gccggacgat cttcgcccga acatactcct cgcctatctc aaagaggcgg tcgagttccc
     2581 ggatcaaggt tgggtcggcc ttgaagttgg ccactacgta gtggccgtcg gtgagatggt
     2641 cgatctcata tgcgaggcga cgtcgtcccc acacatcgag tttggttatc tcacccctgg
     2701 tctcgatgac cttggtgaag cggtcaaccg cctgctggat ggctgcttcg tccagtcgcg
     2761 ggtcgacgat aaccacggcg tcataatttc gcactaactg cctcctgtgg acatccgccc
     2821 aggtcagacc cgtccggaag gcccccgatc acgtcgggag caggttgctc ttgagccgtc
     2881 gcatcgtacc agcgactttt cgttgcgtcc atcggagacg actataggtg gggggtgtga
     2941 cagtcggaac cgaggccgcg aaactttccc gcatctcgcg aaactttcta cgctagcgta
     3001 ggtcgttacg gccaaaggcc tcatcgacga acctctgggc atgaacgggc gtggcagaat
     3061 ccgtatgtgg agtcctacaa cggcaacctt cgaagagaac ctgctgaagc tcaacgtaac
     3121 ggactaagtg gatgggcgag cagtttcgcg aagaggtacc taccggatca gcacatcctc
     3181 ccttgagcga tctcgatagt cgccagcgtt cagcgcggat cctggaagaa gtaaagggac
     3241 ttgccttgcc ccctttcgag aaagccttga tcgcggccac acgactgcac gatgggcagt
     3301 ttcgcaagat gagtgaagaa tcgcggg
//
LOCUS       DATDOZ010000202         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_421715, whole genome shotgun sequence.
ACCESSION   DATDOZ010000202 DATDOZ010000000
VERSION     DATDOZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421715"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VI541_04070"
     CDS             complement(<1..579)
                     /locus_tag="VI541_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLF70111.1"
                     /translation="MGLGRPDPQNIVIFGASGDLTSRKVMPALYNLFVQGLLPEDFCI
                     VGYARTPMAQGAFIDLMRDAVGANSRCELDDDPWAEFSSQLRYVSGEYADADGLGELI
                     PNLDGGRKSLFYCATPPSLFPVIAQALGALDLAHGSRIVIEKPFGRDLDSAKELNGIL
                     HENFAEEQIFRIDHYLGKETVQNILVFRFANGM"
     gene            complement(584..940)
                     /locus_tag="VI541_04075"
     CDS             complement(584..940)
                     /locus_tag="VI541_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70112.1"
                     /translation="MPREYQAELISAKVMPVAHPFGGGIEIKDGETTPFIVERNWAAP
                     AGHYIEEWSIRDGAKVLHRSGSRSIFIRGLQSSTVHRDEVAEPLALPVGDFQLVLVVE
                     GRFMGSVEVTSAPAGA"
     gene            complement(1066..>2340)
                     /locus_tag="VI541_04080"
     CDS             complement(1066..>2340)
                     /locus_tag="VI541_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLF70113.1"
                     /translation="IDASVALCNIEWRNRTTVKHALAATLIKDLNHRPIFESLFDIYF
                     AGVPDDVQMPSPPGPQPLDDMSAELLEALMSMDLERLHDLAAEAIRRYGGMQPGRPVG
                     GRYYAYRVEQSLRLGELTAELERMLGGGGSLEGGFAARRAAVDADVRVSGFKREVERI
                     IRERLLADRGPDAMARALVRPLPEEVEFLQASSEELAEMRRIVSLLGRRLAKRLSYKH
                     HHAKRGRLDFRKTIRASLGTGGTPASPMFKHRVRMPELVVICDVSGSVAAFSRFALAL
                     LYSLSEHFRKVRAFAFVDEIDEVTSYFESADLEEATRRIRTEANVVWIDGHSDYGHSL
                     KSFVTRYEDAVSPRTTVLVLGDARSNFRAPEAGALKQIRERARKLYWLNPEPHRHWNT
                     GDSIASAYEDACDGVYECRNLRQLAAFIERIA"
BASE COUNT          468 a          719 c          699 g          454 t
ORIGIN      
        1 catcccgttc gcgaatcgaa acaccaggat gttctgtacg gtctccttgc ccaagtagtg
       61 gtcgatgcgg aagatctgct cttcggcgaa gttttcatgc aggatcccat tcaactcttt
      121 cgccgagtcg aggtcgcgcc cgaaaggctt ctcgataacg atgcgcgagc catgcgcgag
      181 gtcgagcgcc cctagggcct gggcgatcac cggaaagaga gaagggggcg tcgcacagta
      241 gaacaggctc tttctcccac cgtcgagatt gggtatcaac tccccaaggc catccgcatc
      301 tgcatactct ccagacacat accggagctg cgacgagaac tccgcccagg gatcgtcatc
      361 cagctcgcac ctactgttgg cgccgaccgc atcgcgcatg aggtcgatga aagcgccctg
      421 cgccatcggt gttcgagcgt acccaacgat gcagaagtct tcgggaagca aaccttgaac
      481 gaagaggttg tagagagctg gcatgacctt gcgcgaggtc aggtcacccg atgctccgaa
      541 tatgacgatg ttctgcgggt cgggacggcc cagtcccatg ccgctaggct ccggccggag
      601 ctgaggtgac ctctacggag cccatgaaac ggccctcgac gactagaacg agctggaagt
      661 cacctacagg gagggcgagt ggttcagcaa cctcatctcg atggaccgta gaagattgca
      721 ggccgcggat gaagatgctc cggctcccgg atcggtgcag gactttggct ccatcacgga
      781 tcgaccactc ttcgatgtag tggcctgccg gtgcggccca gttccgctcg acgatgaagg
      841 gtgtggtctc tccatccttg atctcgatcc caccgccgaa tgggtgagcc actggcatga
      901 ccttggccga gatcaattca gcttgatatt cgcggggcat cattcctccg ttagcgcggt
      961 tctctgacga tgataaccgt cacatgcggt gtctctcgaa tcgacgtagc tttctcgggc
     1021 ggattaccac cggtaggcga gggtttttcg cccgagaacg agacatcagg cgatacgttc
     1081 tatgaacgct gctagctgtc gcaggttgcg gcactcgtag acaccgtcgc atgcgtcttc
     1141 gtatgcgctg gcgatggagt cgccggtatt ccagtggcgg tgcggctctg ggttcagcca
     1201 gtacaacttg cgggcccgct cacggatctg cttcagggcc cccgcttcgg gggccctgaa
     1261 gttcgaccgg gcatcaccaa gaaccaatac cgtcgtacga ggcgagacag cgtcctcgta
     1321 tcgagtcacg aatgacttga gcgagtgccc atagtcagaa tggccatcta tccagaccac
     1381 attcgcttcg gtcctgatcc gccttgttgc ctcttcgagg tcggccgatt cgaaatagga
     1441 ggtgacctca tcgatctcat cgacgaaggc gaatgcacgg accttgcgaa agtgctccga
     1501 gagcgagtag agaagcgcga gcgcaaaccg actgaacgct gctaccgaac ccgagacatc
     1561 acagatgacg acgagctccg gcatgcggac ccggtgtttg aacatcggtg aagcaggggt
     1621 ccccccggtg ccgagcgagg ctcgaatagt cttgcggaag tcgagccgac ctcgcttcgc
     1681 atgatggtgc ttgtacgaga gacgtttggc caggcgtcgc cccagcagcg acacgatcct
     1741 tcgcatctcg gccagttcct cggaactggc ttgcaggaac tcgacctcct ccgggagggg
     1801 acgaaccagc gccctcgcca tggcatcggg cccccggtcg gcaagaagac gctcgcggat
     1861 gatcctctcg acttcacgct tgaacccact gacccgcaca tctgcatcga ctgccgccct
     1921 gcgagcagcg aacccccctt cgagcgaacc gccgccgccg agcatgcgct ccagctccgc
     1981 ggtgagctct cccagacgca gcgactgctc cacccggtac gcgtagtagc gccccccgac
     2041 tggtctgcct ggctgcatcc ccccgtaccg ccggatcgcc tctgcagcga gatcgtgaag
     2101 tctctcgaga tccatcgaca tcaacgcttc gagcagttcg gccgacatgt cgtcgagtgg
     2161 ctgggggcct gggggggaag gcatctgtac gtcgtcgggg accccggcga agtagatgtc
     2221 gaatagcgac tcgaatatcg ggcgatgatt caggtccttg atcagcgtcg ctgcaagggc
     2281 gtgcttcacc gtggtgcggt ttcgccattc gatattgcaa agagcaacgg aggcatcgat
//
LOCUS       DATDOZ010000203         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1235770, whole genome shotgun sequence.
ACCESSION   DATDOZ010000203 DATDOZ010000000
VERSION     DATDOZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235770"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2076
                     /locus_tag="VI541_04085"
     CDS             127..2076
                     /locus_tag="VI541_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLF70114.1"
                     /translation="MGLGVRPRDRENAAAAAELVCVAIVTLIPLAFLPSGFFIFLPIK
                     WALGLALTVAGLALVIARGRFRLRTLTPWGAFLVVAAVATAASVDRVTSFIGSPTRNM
                     GLITWALFAAAFSLGASASPRERRSILIAACGASMLVSVYGVLQAIGVDFIDWQSRLD
                     VTRSRSTFGNAAFLGGYLVLVIPLAVRLAFEEAKRATRFMLGAAAVLASACLLTTRTR
                     GAWVGAAVAIAIVVMLERKALKRNAKIAITIACLAAIAIVVTVAPFRARVASIADISQ
                     GTARGRIIQWHLTTELISARPLFGWGPDTLRTEFPDVIDDRFEREVGRDVVPDRAHNV
                     YLDIAAGFGIIGVLAYLFLQVSVLRRVIRERTPVTVAIFGGLVGYIVQSLFSFPVADV
                     DLIFWLFAGLGVCLGFGPKERTAAKGAARRERRDRKTTHLVAAWALGVLALAVLVWSG
                     REVRADLLLGRGLRLQDSNPLAAAALIDRSARLVPERVQYSQASARFRTRLGGARRSD
                     SDFEKALATLGGALRRYSRDGELRLDRADLFLVWGEMNGDAGMTARAERAYRELLRND
                     RSSSRVYLKLGVALVEQQRFVEAETAWRRAAFLAPRHATPHVNLGLLYETIGRVDDAR
                     DEFEKALRIEPNNSGADEGLRRLNQ"
BASE COUNT          372 a          661 c          711 g          472 t
ORIGIN      
        1 gtgcggcctg actggtgtac ctgtccatgc gatatcgctc atggggcctc cctttaaaac
       61 gacggaccga ccgcacgata ctccgctgac tgcgtagagg cacaaaagta tgcgagtctt
      121 cgacagatgg gtcttggagt cagaccgcgt gaccgggaga acgctgctgc ggccgcggaa
      181 ctcgtttgcg ttgccatcgt cacgctgatc cccctcgcct tcctaccgag cggcttcttc
      241 atcttcctgc cgatcaagtg ggcactcggg cttgcactga cagttgcagg gctcgcgctt
      301 gtgatagcaa ggggaaggtt tcgactcagg acgctcaccc catggggggc cttcctcgtc
      361 gtagcggcgg tggcgaccgc ggccagtgtc gatcgcgtga cgagtttcat aggtagccct
      421 accaggaaca tgggtctgat cacctgggct ctcttcgccg ctgcattctc acttggggcg
      481 tccgcctcgc cacgggaacg ccgctcgatc ctcatcgctg cgtgcggggc ttccatgctc
      541 gtctccgtat atggcgtgct ccaggccatc ggcgtcgact tcatcgattg gcagtcgagg
      601 ctcgatgtga cacgaagcag gtcaacgttc ggcaacgccg cgttcctcgg tggatatctc
      661 gtgctggtga tcccactcgc ggtgcgactc gccttcgaag aagccaagcg ggcaacgcga
      721 ttcatgcttg gcgcggccgc tgtcttagca tcggcatgtt tgctgacgac tcggacccga
      781 ggtgcgtggg tcggcgcagc cgtcgcgatc gcgatcgttg tgatgctgga acgcaaggca
      841 cttaagcgca acgcgaagat cgctattacc atcgcatgcc tggcagcgat cgctatcgtg
      901 gtcacggtcg cgccctttcg cgcaagggtc gcatcgatcg cagacatatc tcagggcact
      961 gcccgcgggc ggatcatcca gtggcatctc acaacagagc tcatctcagc gcgtccgctg
     1021 ttcgggtggg ggcccgatac actccggacc gagtttcccg atgtcatcga tgaccggttc
     1081 gagcgtgagg tcgggcgcga cgtcgtaccc gaccgtgccc acaacgtcta cctcgacata
     1141 gcggcagggt tcgggatcat cggggttctc gcctatctgt tccttcaggt ttcagtcctt
     1201 cgacgtgtga tccgtgagcg cacgcctgtc acggtggcca tcttcggggg ccttgtcggc
     1261 tacatcgttc aatccctgtt cagcttccct gtcgccgatg tcgatctgat cttctggtta
     1321 ttcgccgggc tgggagtctg cctggggttc ggtccgaaag agcgaaccgc tgccaagggg
     1381 gcagcccgtc gcgagaggcg cgatcggaag accactcatc tcgtcgccgc ttgggctctc
     1441 ggcgtccttg ctctggcggt cctcgtctgg tctggtcgag aggttcgagc cgaccttctg
     1501 cttggtcggg gacttcgcct tcaggatagt aacccactcg ctgcggctgc tctcatcgac
     1561 agatctgcaa ggttggtacc cgagcgcgtt cagtactctc aagcgtcagc gcgtttccga
     1621 accagactgg ggggggcgag gcgaagcgac tccgacttcg agaaggccct tgcaacactg
     1681 ggcggtgcgc tccgacgcta ctcacgagat ggggagctga ggctcgatcg agccgacctg
     1741 ttcctcgtgt ggggagagat gaacggcgat gctgggatga cagcgcgggc agagagggct
     1801 tacagggaac tgctccgaaa tgaccgttcc tcttcaaggg tctatctgaa gcttggagtt
     1861 gccctcgtcg aacagcagcg attcgtcgaa gcggaaacgg cgtggcggag ggctgcgttt
     1921 ctcgcgccaa ggcatgcgac cccgcacgtg aatctcggct tgctgtacga gaccatcgga
     1981 agagtcgatg atgcccgaga tgagttcgag aaggcactgc ggatcgagcc gaacaactcg
     2041 ggagcagatg aaggtttgcg gagactgaat cagtgaagcg gatcgtcgtg gtgagcgtgg
     2101 gcctgttact tgctgtcgcc gggttcttgg tgctgcgccc tcgcgaggcc cccgatgcga
     2161 ctgctttctc gcggttcctt gcgacctggg gagacgcacg ggggccgagc ttcgca
//
LOCUS       DATDOZ010000204         6099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2276298, whole genome shotgun sequence.
ACCESSION   DATDOZ010000204 DATDOZ010000000
VERSION     DATDOZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276298"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VI541_04090"
     CDS             complement(<1..645)
                     /locus_tag="VI541_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70115.1"
                     /translation="MSTDFDAPTSDDPFQTRIDALLSSALEDQAREQKMLVETVKGAR
                     ASIVRTEEELSELRRILQDRDRSVLDLLEARLSGIGSQETIEQVDGRLAELLSRSSTE
                     QAVAPINASLTALTQRLSSLESEVSRTDPHDALRELRSGLESRIEGSLHPVRASVEEL
                     ARVVGELEAPIRDVIEGPMSSLRESVESVRQGAVADVGAMLARLEAVEARVAERL"
     gene            complement(673..3033)
                     /gene="cofH"
                     /locus_tag="VI541_04095"
     CDS             complement(673..3033)
                     /gene="cofH"
                     /locus_tag="VI541_04095"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HLF70116.1"
                     /translation="MDRIGRVEAQQLAAAFAARGLQPEVLDRAAQIRDERSGRVITYS
                     PKVFIPLTKLCRDVCHYCTFARPPVAGEAAFMTIDEVLEVARAGQAAGCAEALFTLGD
                     KPELRYRVARDELAQLGHSTTIEYLVSAAHEVFEQTRLLPHLNPGVVTRDELVEMKKV
                     SASMGLMLESTSTHLMERGGAHFGSPDKEPSARLAMLEAAGELKIPFTTGLLIGIGEG
                     WADRIETLFAIAESHERWGHVQEVIVQNFKAKPDTLMHSHPEPSIEEMVMTIALARLI
                     LPADVSVQAPPNLAGDDAVMLQYLGAGIDDWGGVSPVTPDHVNPEAPWPHIAQLRAIT
                     ESSGHLLLPRLTVYPRYVHDGEWIARDIRGGVLAKADTSGLLRDDRWHPGESFDPPDV
                     ITRPWGWSGSDDVLCRPEFMHALDRARQGEELARSDVLALFEARGPELPLLFGAADSL
                     RQRVNGDTVTYVINRNINYTNMCYFKCGFCAFSKGPASLNLRGEPYLLDLEEIAHRAL
                     EAAAAGATEVCLQGGIHPSFTGDFYLQVIDAIKAVVPDMHIHAFSPLEVWQGAQTKGV
                     SIRDFLHELKDHGLSTLPGTAAEILDDRIRSIICPDKINTDQWSSVARTAHSLGLRMT
                     TTIMFGHADDYASWVSHLNVLRDIQRDTGGFTEFVPLPFVHMGAPIFLRGKARRGPTI
                     EETLKVHAVGRLYLHPFITNIQMSWVKLGERGAEMALAAGCNDLGGTLMNENISRAAG
                     AAHGQEMTPQTMESLARRAGRPLARRNTVYDILEGPVVESGEPIVR"
     gene            3067..3987
                     /gene="cofD"
                     /locus_tag="VI541_04100"
     CDS             3067..3987
                     /gene="cofD"
                     /locus_tag="VI541_04100"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HLF70117.1"
                     /translation="MRVTALAGGVGAAKFLRGLVQVMDPSDLTIIGNVGDDATFHGLE
                     VSPDLDIVAYTLSGQIAASGWGFEGDTTSALDQMRSYGIDGWFTLMDRDLGTHLARTT
                     WLREGIALSEVTTRICAGLGISARLIPATDDRLRTVLTVKAGIRREFQEYFVKHRHEE
                     EVTSVTFDGSETARPAPGVLDAIETADLILISPSNPVISVGPILAVKGIRETLRARRK
                     NVTAISPIVEGAALKGPADRLLPLLGAEVSASGVAGLYQDFCSSFIVDERDPQEAGKV
                     AALRMTPHLLDTVMTDPSVAARLAKEILAL"
     gene            3984..4718
                     /gene="cofE"
                     /locus_tag="VI541_04105"
     CDS             3984..4718
                     /gene="cofE"
                     /locus_tag="VI541_04105"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HLF70118.1"
                     /translation="MKQPNYSAPRGPVTILPVLGIGEIKEGDDVAASIAAALTKLGIQ
                     LTDDDVVVVTQKIISKAEGRVVAVPVGDDDARHRIAEQEAKQVLRRRDGLIITETHHG
                     FVCANAGVDASNIDEGYVVLLPKDPDLSARRICKRLANITGASPAVIISDTFGRAWRN
                     GQTNVAIGVAGMLPLLDYMGTHDHFGRELKVTSIAVADELAGAAELVMGKADGVPVAV
                     IRGAYITKGSGDAKALIRQPATDMFR"
     gene            4729..5391
                     /locus_tag="VI541_04110"
     CDS             4729..5391
                     /locus_tag="VI541_04110"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HLF70119.1"
                     /translation="MNDQTKKERREAAHKARLELQKARQRARRRKKISLIAVIALVVG
                     VLGYLGVTSYIEGKKTEKQVLSTIKQSWTQAGCTDIRKITELPASPHLTQTDPPPSYN
                     SNPPTSGQHFGTTAPWGFVDQKIDDRITVHNLEHGGIVIHYRQIPSSDVSELRDLVDE
                     VGDGIVLEPDPAIKDKLAMASWGRLQTCEKVDLKVVRFFVKQNCNKGPEDLALECSKV
                     KV"
     gene            complement(5412..>6099)
                     /locus_tag="VI541_04115"
     CDS             complement(5412..>6099)
                     /locus_tag="VI541_04115"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF70120.1"
                     /translation="QIDVRVSVADPVKDANINIAGTLAVLEAARAVGARFVFTSSGGC
                     IYGEAEEPPVDESATGRPTSPYGISKRVIADYLHFYALTHGLRSVVLALANVYGPGQD
                     PSGESGVVAMFAGRLLAGERCYIYGDGSATRDYVYVSDVVDAFKTAATLGDGELFNIG
                     TGAETSVSELYGLMKGITGSDLEPIFADARPGELQRSALDARKAAKILGWAPKVPLEE
                     GLRLTVDSFR"
BASE COUNT         1244 a         1885 c         1846 g         1124 t
ORIGIN      
        1 gagccgctcg gccacgcggg cctccaccgc ctccaacctc gccaacatgg cgccgacatc
       61 agcgacggct ccctgtctga ccgactcgac gctctcacga agtgaggaca tcggcccttc
      121 gatcacgtca cggataggag cctccaactc gccgacgacc cttgccaact cctcgacaga
      181 ggcccgtact gggtgaagtg agccctcgat acgagattca aggccagagc gcagctcgcg
      241 cagggcatca tgcggatcgg tccgcgatac ctcggactcg aggctcgata gtctctgtgt
      301 gagcgcggtc aaggaagcgt tgatgggcgc gacggcttgc tctgtcgaac ttcggctcag
      361 cagttccgcg agccgaccgt caacctgctc gatggtctcc tgagatccga tgcccgagag
      421 gcgggcctcg aggagatcga gaacggatcg atctcgatcc tgaaggatgc gtcgaagttc
      481 tgagagctcc tcttcggtgc ggacgatcga ggccctcgcc cccttgactg tctcgacgag
      541 catcttctgc tcgcgggcct ggtcttcgag cgcggaagat agaagcgcat cgatgcgcgt
      601 ctggaacggg tcgtcagatg tgggagcgtc gaaatcggtg ctcatgaagc cccaatgcta
      661 ttgagtactg ccctaacgca caatcggctc gccagactcg acaactgggc cctccaggat
      721 gtcgtagacc gtgttgcgcc tggccagggg tcgccctgcc ctgcgagcca gagattccat
      781 ggtctgtggc gtcatctcct ggccgtgggc agccccagcc gcgcgagaga tgttctcgtt
      841 catgagggtt ccccccagat cgttgcaacc tgcggctaga gccatctcgg cgccgcgctc
      901 ccccagcttc acccaagaca tctggatgtt cgtgatgaag ggatgaagat acagacgacc
      961 gaccgcatga accttgagag tctcctctat ggtggggcct cggcgagctt tgcctctcag
     1021 gaatatcggg gcccccatgt gcacgaacgg cagcggaacg aactcggtga agcccccggt
     1081 gtcgcgctgg atgtcgcgaa gcacgttcaa atgagagacc caagatgcgt agtcgtcagc
     1141 atggccgaac atgatcgtcg tcgtcatccg aaggcccagc gaatgagccg tcctcgccac
     1201 ggatgaccac tgatcggtgt tgatcttgtc cgggcagatg atgctgcgga tcctgtcgtc
     1261 gaggatctcg gcagccgttc ccggcagagt ggacaggccg tgatctttca actcgtgcag
     1321 gaaatctcgg atgctgactc ccttggtctg cgccccctgc caaacctcga gcggactgaa
     1381 cgcgtggatg tgcatatcag ggacgacggc tttgatcgcg tcgatgacct gcagatagaa
     1441 atctcccgtg aatgacgggt gtatcccgcc ctggaggcag acctctgtgg ccccggcggc
     1501 tgcggcttcg agggctcgat gagcgatctc ttcgaggtcg agaaggtagg gctctccgcg
     1561 aagattgagt gacgccggac ccttgctgaa cgcgcagaaa ccacacttga agtagcacat
     1621 gttggtgtag ttgatgtttc gattgatgac ataggtaacg gtgtcgccat tgacgcgttg
     1681 acgcaacgag tcggcagcgc cgaagagcag gggcagctcg gggcctctcg cctcgaacag
     1741 ggccagtaca tcgcttcggg caagttcttc tccttgccgc gctcggtcca acgcatgcat
     1801 gaactcgggc cgacacaaca catcgtcact cccgctccac ccccacgggc gagtgatgac
     1861 atcgggtggg tcgaaggatt cgcccggatg ccagcgatcg tcccgcagta gtccagacgt
     1921 gtcggccttc gcaagaacgc cgccgcggat atctctggcg atccattccc catcatggac
     1981 gtagcgcggg tagacggtga gccgcggaag taacaggtgc cctgagctct cggtgatcgc
     2041 gcgaagctgt gcgatgtgcg gccaaggagc ttcggggttt acgtggtctg gggtcaccgg
     2101 cgagacgccg ccccagtcgt cgatcccggc ccccaggtac tggagcatca ccgcatcatc
     2161 acctgcgagg ttcgggggcg cctgaacact gacatcggcc ggaaggatga gccgggccag
     2221 ggcgatggtc ataaccatct cttcgatgct cggttcggga tgagaatgca tgagggtgtc
     2281 cggcttggcc ttgaagttct ggactatcac ctcttgaaca tgaccccatc gctcatgcga
     2341 ttcggcgatc gcgaagagag tctcgatgcg gtcggcccac ccctcgccga tgccgatgag
     2401 aaggccggtc gtgaagggga tcttgagctc gccggctgct tcgagcatcg ctagccgcgc
     2461 cgagggctcc ttgtcaggag agccgaagtg ggccccgcct ctctccatca gatgcgttga
     2521 ggtggactcc aacatcaacc ccatcgaggc cgagaccttc ttcatctcga ccaactcatc
     2581 gcgcgtcaca actccggggt tcaagtgcgg cagaaggcgt gtctgctcga acacctcatg
     2641 cgccgcactg accagatact cgatcgtcgt ggagtggcct aactgcgcaa gttcatccct
     2701 cgcgaccctg tagcgcaact cgggcttgtc gccaagggtg aagagagcct cggcacaacc
     2761 ggcagcctga cccgctcgcg cgacctcgag gacctcgtcg atggtcatga aagcggcctc
     2821 gcccgcgacc gggggcctgg cgaaagtgca gtagtgacag acatcgcggc agagcttggt
     2881 cagagggatg aagactttcg gcgaataggt gatgacccgc ccactgcgct cgtctcggat
     2941 ctgtgccgct cgatcgagaa cctccggctg aagcccgcgg gcagcaaagg cagctgccaa
     3001 ctgctgagct tcgacccttc cgatgcggtc cacttggtgt cgaatcttat tgatactctg
     3061 agcggcatgc gtgtaacagc acttgccggg ggtgtgggag cggcgaagtt ccttcgggga
     3121 ctcgtccagg tgatggaccc atccgacctc acgatcatcg gaaacgtagg cgacgacgcc
     3181 acgttccacg gccttgaggt gagcccagat ctcgacatcg tcgcctacac gctctcgggc
     3241 cagatcgccg cctctgggtg gggtttcgag ggcgacacca ccagtgcact cgaccagatg
     3301 cgttcctatg gaatagatgg ctggttcaca ctgatggatc gtgacctggg cacccatctg
     3361 gcgcgaacaa cctggctccg ggaggggatc gcactctcag aggtaacaac caggatctgc
     3421 gcgggactcg ggatctcagc acggcttatc cccgccaccg acgaccgcct gcggaccgtg
     3481 ctaactgtga aggcagggat ccgccgagag ttccaggagt acttcgtgaa acatcgacat
     3541 gaagaggaag tgacctcagt gacgttcgat ggttcagaga ccgctcgccc ggcccccggg
     3601 gtgctagatg ccatcgagac ggccgacctg atcttgatct caccctcgaa cccggtcatc
     3661 agcgttgggc cgatccttgc cgtgaagggg atccgagaaa cacttcgggc cagaagaaag
     3721 aacgtaactg cgatatcccc gatcgtcgag ggtgcggcct tgaaaggtcc ggccgacaga
     3781 ctgctcccgc tattgggggc cgaggtgtcg gcatcgggtg tggcaggtct ctaccaagat
     3841 ttctgttcca gcttcatcgt cgatgagcgc gatccgcagg aggccggcaa ggttgccgcc
     3901 ttgcggatga ctccgcacct cctcgacacg gtgatgaccg acccctcggt cgccgcacgt
     3961 cttgccaaag agatactcgc gctgtgaagc aacccaacta ttcggccccc agggggccgg
     4021 tcaccatcct gcccgtgctt ggtatcggcg agatcaaaga gggggacgat gtcgccgcct
     4081 cgatcgcagc cgccttaact aagttgggga tacaactcac cgacgatgac gtggtggttg
     4141 tcacacagaa gatcatctcg aaagccgagg gccgcgtcgt cgcagtgccc gtaggtgatg
     4201 acgatgcgcg tcatcgcatc gccgaacagg aggccaagca ggtgcttcgt cgccgagatg
     4261 gattgatcat caccgagacg catcacgggt tcgtctgcgc aaacgcaggg gttgacgcat
     4321 cgaacataga cgaaggctat gtcgtcctcc tcccgaaaga ccctgacctg tcggcccggc
     4381 gtatctgcaa gagattggcg aacatcaccg gagcatctcc cgcggtgatc atctcggaca
     4441 ccttcggtcg ggcatggcgt aacggtcaga cgaacgtcgc gatcggcgtc gcgggcatgc
     4501 tcccactgct cgactacatg ggcacccatg accacttcgg ccgagagctg aaggtgacct
     4561 cgatcgccgt cgcagacgag ttggcgggag cagccgaact ggtgatgggt aaggcagacg
     4621 gggtccccgt cgcggtgata aggggggcgt acatcaccaa aggtagtgga gatgccaagg
     4681 ccctcatccg ccagcccgcg accgacatgt tccgctagta tctggcgcgt gaacgatcag
     4741 accaagaagg agcggcgcga ggccgcccat aaagccagac tcgaacttca gaaggcacgc
     4801 cagcgcgccc gtcgacgcaa gaagatatct ctgatcgccg tgatcgctct ggtcgtcgga
     4861 gtcctcggct acctcggcgt cacctcatac atcgagggga aaaagactga gaaacaggtg
     4921 ctctcgacca tcaagcagag ctggacccag gcaggttgca ccgatatccg caagatcact
     4981 gagctccccg cctcgcctca cttgactcag accgatccac cgccgtccta caactccaac
     5041 cccccgactt caggccaaca cttcggaact acggccccct ggggtttcgt cgaccagaag
     5101 atcgacgacc gcatcaccgt tcacaacctc gaacacggcg gcatcgtcat ccactacaga
     5161 cagatcccat cctccgacgt atcggaactg agggacctgg tcgacgaggt gggcgacggc
     5221 atcgtcctcg agcccgaccc ggcgatcaag gacaaactgg cgatggcctc atggggtcga
     5281 ctccagacct gcgagaaggt cgatctgaag gttgtgcggt tcttcgtgaa gcagaactgc
     5341 aacaagggcc ccgaagacct ggcactcgaa tgcagcaagg tcaaggtata gggagcacct
     5401 aacttcaagg cttaccgaaa cgaatcgacg gtaagcctca gcccctcttc aagtgggact
     5461 ttgggggccc agccaagtat cttggcggcc ttacgggcgt cgagggcgct tcgttgaagt
     5521 tcaccagggc gtgcatcggc gaagatgggc tcgaggtctg acccggtgat ccccttcatc
     5581 agaccataca gttcagacac gctcgtctcg gcgccggtcc cgatgttgaa cagctcccca
     5641 tcgcccaaag ttgccgctgt cttgaaggca tcaacgacgt ccgagacata gacgtaatcc
     5701 cttgttgcgg aaccatcgcc gtatatgtag cagcgctcgc cggcgagtaa tctccccgcg
     5761 aacatggcga caacccctga ctcgccggaa ggatcctgtc ccggtccgta gacattagcc
     5821 agagcgagca cgacactgcg caggccgtgg gtgagcgcgt agaagtgaag ataatcggcg
     5881 atgacccttt tgctgatccc gtaaggcgag gttggccgac ccgttgctga ctcatcgacg
     5941 ggaggttcct cggcctctcc gtagatgcaa ccaccagatg aagtgaagac gaaccgggcc
     6001 cccaccgccc gtgctgcttc cagaaccgca agagtgcccg cgatattgat gttcgcgtcc
     6061 ttcaccggat cggcaacaga cactcgcaca tcgatctga
//
LOCUS       DATDOZ010000205         3419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1749456, whole genome shotgun sequence.
ACCESSION   DATDOZ010000205 DATDOZ010000000
VERSION     DATDOZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749456"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VI541_04120"
     CDS             <1..1526
                     /locus_tag="VI541_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLF70121.1"
                     /translation="AEVGEPGQYPYTRGVYPTMYRGKLWTFRQFSGFGSPEETNARYR
                     FLIEQGQTGLSVAFDMPTLMGRDSDDPMSQGEVGKCGVAVDSVADMETLFRDIPLGDI
                     STSMTISGPAVMIFAMWLVVAERRGVPWALLDGTLQTDILKEYIAQKEWIYPPEPHLR
                     MTADLMKFCRDQVPRYHPLSISGYHIREAGSTAVQELAFTLASGFAHVELGVRNGMTA
                     DEVASALSFFFNSHIDFFEEIAKFRAARRIWAKWMKSRYGCTNEKSLMMRFHTQTAGC
                     SLTAQQPYNNVVRTSFEALAAVLGGTQSLHTNSLDEVLALPTEEAAEIALRTQQIIAY
                     ETGTTNVIDPLGGSWFVESITNRTEAEAQEYFGRIDELGDGSILDGMLRGIEEGYFQQ
                     EIADAAFRYQNLLDAGRKYMVGANKFVRPGADGEIDLLVIDEEGERRQVKDLQARKTD
                     RDQGKVDAALAALVSVARSDANIIPAFIDAARVEATLGEMVDALKEPFGIYREPPRF"
     gene            1528..2532
                     /locus_tag="VI541_04125"
     CDS             1528..2532
                     /locus_tag="VI541_04125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLF70122.1"
                     /translation="MKRLGVNIPLEGLPVEESVVPLARFAEALGYTDVWSAESGHVDG
                     LTPLAAVAATCPSLRLGTAILPIYTRPPALMAMSAAGLQLLSGGRFVLGLGTSTSIIV
                     ERWMGQSFTKPLARVRETVGVIREALAGKKVTFQGESISLDGFRLSTGEVTDVPIYIA
                     ALGPKMLRLAGEIADGVILWLFTPDGVGEALKHVREGAAAAGRDPDSIDVVMRIVVAV
                     DEDPEVLSYMMRRLATTYVMVDVYNASIRRQGFAKEADEVRRLWDAGDREGAAAAISD
                     ELLNGIFITGDHDSCRAKLERFREVGIKTPVLFPVSVDGDPAVRVEKARHVLEVMAGA
                     "
     gene            complement(2529..2729)
                     /locus_tag="VI541_04130"
     CDS             complement(2529..2729)
                     /locus_tag="VI541_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70123.1"
                     /translation="MFDLLGKMIIGIRVLAASPSPIFPTLPADAPPPGVSSPLAGVIA
                     IVAILVVAVAGIAIYRVIRKGL"
     gene            2774..3049
                     /locus_tag="VI541_04135"
     CDS             2774..3049
                     /locus_tag="VI541_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70124.1"
                     /translation="MTTATIATIAAVDVPPPVSLLSPFEVMVPVTGGCDEEGPTDTSG
                     GSPGDALVVIDGDGLGEGVGLGEGEALGGLLGDGDGLGDGDGLEPEP"
BASE COUNT          665 a         1011 c         1086 g          657 t
ORIGIN      
        1 aagccgaggt tggggagccg ggccagtacc catacacgcg cggcgtctac ccaaccatgt
       61 atcgcggaaa gctctggaca ttccgacagt tctcgggatt cggcagcccc gaggagacca
      121 acgctcgcta cagattcctg atcgaacagg ggcagacggg cctctcggta gcattcgaca
      181 tgccgaccct catgggccgc gactccgacg accccatgtc gcagggcgag gtcggcaagt
      241 gcggcgtcgc agtcgattca gtcgcagaca tggagactct attcagagac atccccctcg
      301 gtgacatctc gacatcgatg accatctcag gaccggccgt gatgatcttc gcgatgtggc
      361 ttgtcgtcgc agagcgtcgt ggcgtgccat gggccctgct cgatggaacc ctccagaccg
      421 acatcctcaa ggagtacata gcgcagaagg agtggatata cccacctgag cctcacctgc
      481 gcatgaccgc ggacctcatg aagttctgtc gcgaccaggt tccccgctac cacccactct
      541 cgatatcggg ctatcacatc cgcgaggctg gttcgactgc ggtgcaggaa ctggccttca
      601 cgttggcttc aggattcgca catgtcgaac tcggtgtgcg caacggtatg accgccgacg
      661 aggttgcctc tgcgctttcg ttcttcttca actctcacat cgacttcttc gaggagatcg
      721 caaagtttcg cgcggcacga aggatctggg cgaagtggat gaagagtcgc tacggatgca
      781 ccaacgagaa gtctctgatg atgcggttcc acacgcagac cgccggatgt tcgctcaccg
      841 cgcagcagcc ttacaacaac gtggtgagaa catcgttcga ggcgttggcc gcggtgctcg
      901 gcggcaccca gtcgcttcac accaactctc tcgacgaggt cctcgccctg ccaaccgaag
      961 aggctgccga gatagccctg cgcacgcagc agatcatcgc ctacgagacc gggacaacca
     1021 acgtgatcga ccctctcggc ggatcatggt tcgtcgagtc gattaccaac cggaccgaag
     1081 ccgaagcgca ggagtacttc gggcggatcg atgaactcgg cgatggttcg atcctcgatg
     1141 ggatgttgag gggcatcgag gagggttact tccagcaaga gatcgcggat gctgccttcc
     1201 ggtatcagaa ccttctagat gctgggcgta agtacatggt cggcgcgaac aagttcgtcc
     1261 gtccgggggc cgatggtgag atagacctcc tcgtcatcga cgaagaaggc gagcgaaggc
     1321 aggtcaagga tctgcaggct cgaaagaccg acagggatca gggcaaggtc gatgcggccc
     1381 tcgcggctct cgtatcagtt gctcgcagcg atgccaacat catcccagca ttcatagatg
     1441 ccgctcgggt cgaggccacc ctgggcgaga tggtcgatgc tctgaaggaa ccattcggca
     1501 tctaccgcga gcccccgcgc ttctaacgtg aaaagactcg gcgtcaacat cccgctcgaa
     1561 ggccttccgg tcgaagagtc ggtcgtacca ctggcccgct tcgccgaggc cctcggatac
     1621 accgacgttt ggtccgccga gtcgggacat gtcgacggcc ttactccact cgcggcggtg
     1681 gcggcgacct gcccttctct tcgtctcggt acggcgatcc ttccgatcta cacgcgcccg
     1741 ccggccctca tggccatgag cgcggcaggg ctccagctcc tctcgggtgg gaggttcgtg
     1801 ttggggctcg gcacatcgac atcgatcatc gtcgagcgtt ggatgggaca gagcttcacc
     1861 aaacccttag cccgcgtgcg cgagaccgta ggcgttatcc gcgaagccct cgctggtaag
     1921 aaggtcacct tccaaggtga gtcgatatcg ctcgatggct tccgcctatc gaccggtgaa
     1981 gtcaccgacg tgcccatata catcgccgct ctcggcccaa agatgctgcg actcgcaggg
     2041 gagatagccg atggagtgat cctctggctc ttcacccccg atggcgtggg cgaggcactc
     2101 aaacatgtgc gagagggtgc cgccgctgcg ggccgggacc ccgactcgat cgatgtcgtg
     2161 atgaggatag tcgtagcagt tgatgaagat cccgaggtcc tgagttacat gatgcggcgg
     2221 ctcgcgacga cctatgtgat ggtcgatgtc tacaacgcgt cgatccgtcg acagggattc
     2281 gccaaagagg ccgacgaggt acgcaggctc tgggatgccg gggatcgaga gggcgccgcc
     2341 gctgcgatct cggacgagct gctgaatggc atcttcataa ccggcgacca tgattcatgc
     2401 agggcgaagc ttgaacgttt ccgtgaggtc gggatcaaga ctcccgtgct cttccccgtc
     2461 tcagtcgatg gagaccccgc ggtgcgcgtc gagaaagcgc gacacgttct cgaggtgatg
     2521 gcgggggcct agaggccctt gcggatgacg cggtagatcg cgatccccgc gacagcgacg
     2581 accaagatcg ccacgatggc gatgactccg gcgagtgggc tggatacacc tgggggcggg
     2641 gcgtctgcag gaagcgtcgg aaagatgggt gagggcgatg ctgccaacac ccggatcccg
     2701 atgatcatct tgccgaggag atcaaacacc gcgtcgccgc aacacccaca acccgatccc
     2761 cgaacccgcc gcgatgacga cggcaactat cgcaacgatc gcagccgttg acgtacctcc
     2821 acccgtctcg ttgctctctc ccttcgaggt gatggtccct gtgaccgggg gctgcgatga
     2881 agagggcccg accgatacct ctggaggctc gcccggtgac gcgctagtgg tgatcgacgg
     2941 tgatgggctg ggggaaggtg tgggactcgg cgagggcgag gccttgggcg gcttgctcgg
     3001 cgatggcgat gggctcggcg atggcgatgg gctcgagcca gaaccctgaa cagtgatcgt
     3061 tccggtcatg tttgggtggt acttgcagtg gtatgggaaa gatccggagg aagcgaatgt
     3121 atggctgaat tcttgatcgg ctgctaaggg gtcttggtcg aaggttgaac cgtcattcga
     3181 cgtaactgta tgagggttat cggccgtgtc gttgttcgtc cagcggaccg tatcgccgac
     3241 atcgatcgtg aggttctgcg ggctgaaggt agcggcggta ctcgccccta cgatgctcac
     3301 gtccctggtt gctgcctggg cgggaggcgt ggcaagcgcc aggagtacta tcgcggcgat
     3361 gaggcccccg agtcgttttg tcatatgagt ttcagcagca cgtctttgat ctccgtagt
//
LOCUS       DATDOZ010000206         3100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2793464, whole genome shotgun sequence.
ACCESSION   DATDOZ010000206 DATDOZ010000000
VERSION     DATDOZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793464"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..431
                     /locus_tag="VI541_04140"
     CDS             165..431
                     /locus_tag="VI541_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLF70125.1"
                     /translation="MGGSDGGQRLARAIGEQIAERRRSKGLSQEGLARMAGMSRETVR
                     RAEAGERMPRWVTLAKLAECLDIRLTDLVALATAVEHPTSGGRR"
     gene            428..2164
                     /locus_tag="VI541_04145"
     CDS             428..2164
                     /locus_tag="VI541_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLF70126.1"
                     /translation="MRAKVKDKVPEHALVMEPATLHVTAPLGEILIERGLIDAAQLKG
                     ALLQQEASGALLGRLLVDLGLIDERALSDTLGEHFGLESVDLRSVDPDEEVLSKISEA
                     DARALGVLPLHMEADELHVVVADPTSQKLSTVSETVECSVRFFVAPASDVRRAIDRVY
                     RATAGLDSAVEAFQSSESIRQRDTLRDDGDAESAPIVQVVNMLIAQGLRDRASDIHIE
                     PQEGRMRIRFRTDGKLRDVLALPPNMGPAVISRIKIMAGMNIVERRRSQDGRIEVKTE
                     GRDLDIRVATSGTIWGEKAVLRLLDKSRPLYRLEELGMPPDVRDAFSRLVHAPFGMVV
                     CAGPTGSGKTTTLYAILKEINHPDVNIITIEDPVEYILPSINQIQINEAAGIAFATEL
                     KAILRQDPDVILLGEVRDVDTARIAVQSTLTGHFVLSSVHATDAAGALHRFLDMGIEP
                     YLITSSVIAVLAQRLVRRICSYCREEYRPTVQESAFLAAVGSDHKKRLVRGTGCNFCG
                     GTGFEDRIGIFELLQVDDEMKQALVENPTHKNLRRIALRGGMHPLQHEAVQLVEAGIT
                     TLSEVMRALYIS"
     gene            2176..>3100
                     /locus_tag="VI541_04150"
     CDS             2176..>3100
                     /locus_tag="VI541_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLF70127.1"
                     /translation="MAQERILTLAPKADRGPKLVRTRGRKVPLQEVMHFSRQLAAFMR
                     AGIPIIDALEVMSEESPKALREMLTAAIEDLKAGESLSDALAHHPKALPAYYPKILRS
                     AEMTGRLDSVLDEVAGYLEREIEARRAIRSAMIYPSAILGMAGVAVVVLTGFVLPRFE
                     RFFVSFNAKLPLPTRILLGIAGFMGRWWWLIVAGLVVTVVGLVLISRTTRGRAARDRV
                     LLRLPAIGDVVRFAVIERVARILASTIRAGVPLPEAMSVTAEGAHNVVYQRALGEVRE
                     AMMRGEGIARPLADTKLFPTSVLQMVKVGEHT"
BASE COUNT          612 a          925 c          969 g          594 t
ORIGIN      
        1 tgtctctttc cggtctcaac tctctagctt caccctcatc taagtatcgg atgacccacg
       61 ttcgccattc aaagcgaact tccgaaggcc catcatgcaa ctccagcggt tgcaactcgc
      121 ggagttgcat gtcaagagcc gcaggtcggt gagggagaac tcccatgggt ggttcggacg
      181 gaggtcagcg gttggctcgg gccatcggtg agcagatagc cgagcgacgc cgaagcaagg
      241 ggctctcaca agaggggctc gcgaggatgg ccgggatgag ccgcgagacg gtcagacgcg
      301 ccgaggcagg cgagcggatg ccgaggtggg tgaccctcgc caagttggcc gaatgccttg
      361 atatccgact taccgatctg gtcgcgctcg ccaccgctgt cgagcatccc acctcaggcg
      421 gtcgtcgttg agagccaagg tcaaggacaa ggtccccgag cacgcactgg tgatggagcc
      481 ggcaaccctt catgtcacgg ctcccctcgg tgagatcctc atcgagcgcg gactcataga
      541 tgccgctcag ctcaaggggg cccttctaca gcaggaagcc tccggagccc ttcttgggcg
      601 gctgctggtc gatctaggac tgatcgacga gcgagccctc tccgacaccc tgggggagca
      661 cttcgggttg gagtccgtcg atcttcgttc cgtcgatccc gacgaggagg tcctgtcgaa
      721 gatctccgag gccgatgcac gcgcattggg agttcttccc ctccatatgg aggccgatga
      781 gctccacgta gttgtggctg atccaacttc acagaagctg tcgactgtca gtgagaccgt
      841 cgaatgctct gtcagattct tcgtggctcc cgcctcggat gtgcgacggg ctatagaccg
      901 ggtttaccgt gcaaccgcag gcctcgactc tgcagttgaa gcgttccaat cgagcgaatc
      961 gatcaggcaa cgcgacacgc tgcgagatga cggcgatgcc gagtccgctc cgatcgtaca
     1021 ggtcgtgaac atgctcatcg cccaggggct gcgagaccga gcctctgaca tccacataga
     1081 gccccaagag ggccgcatga ggatccgatt ccgcacagac gggaaactgc gcgacgtcct
     1141 ggctctgccg ccaaacatgg gcccggcagt catcagccgg atcaagatca tggcgggcat
     1201 gaatatcgtc gagcggcgtc gttctcagga cggtcgcatc gaggtcaaga ccgaggggcg
     1261 cgatctcgac atccgagtcg caacgtccgg gacgatctgg ggagagaagg cggtcctgcg
     1321 gctgctcgac aagagccgac cgctttatcg gttggaagaa ctgggcatgc ctcccgatgt
     1381 ccgggacgcg ttcagtcgtc tcgtgcatgc ccctttcggc atggtcgtct gcgcgggacc
     1441 caccggaagt ggaaagacga ccaccctcta cgccatcctc aaagagatca accaccccga
     1501 cgtgaacatc atcacgatcg aggacccggt cgagtacatc ctcccttcga tcaaccagat
     1561 ccagatcaac gaagcggcgg gcatcgcctt cgcaacagaa ctgaaggcga tcctgcgcca
     1621 agaccctgat gtgatcctcc tgggtgaggt tcgcgatgtc gacacagcgc ggatcgccgt
     1681 gcaatcgacc ctcacaggcc acttcgtcct ctcttccgtg cacgcgacag acgcggccgg
     1741 agctctgcac agattcctcg acatggggat cgagccctac ctgatcacct cgtcggtcat
     1801 cgcagttcta gcccagagac tggtgcgaag gatctgcagt tactgccgcg aggagtatcg
     1861 accgaccgtg caggagtcag cgttccttgc ggcggtgggg tccgatcaca agaaacgact
     1921 ggtgcgaggc acaggttgca acttctgcgg gggcaccggc ttcgaagatc ggatcgggat
     1981 cttcgagcta ctgcaggtcg atgacgagat gaagcaggcc ctcgtcgaga accccactca
     2041 caagaatctg cgtcgcatag cgcttcgagg agggatgcat ccccttcagc acgaggccgt
     2101 gcagctcgtg gaggcgggga tcacgactct ttcagaggtc atgcgggccc tttacatcag
     2161 ctaggaggaa tcgagatggc tcaagaaagg atccttaccc tcgcaccgaa agccgacagg
     2221 gggccgaagc tcgtgaggac cagaggccgc aaggttccgt tgcaggaggt gatgcacttc
     2281 tcgcgacaac tcgctgcatt catgagggca ggcatcccga tcatcgatgc gctcgaagtg
     2341 atgtcggagg agtcgccgaa ggctcttcgt gagatgttga cagcggcgat cgaggacctc
     2401 aaggctggcg agagcctgtc ggatgccttg gcgcatcatc ccaaggcgct tcctgcttac
     2461 tacccgaaga tccttcgctc agctgagatg actggaaggc tcgactcggt cttggatgag
     2521 gtcgccgggt acctggagcg agagatcgag gcccgtcgcg ctatccgctc tgccatgatc
     2581 tacccgagcg cgatcctagg gatggctggc gtcgcggtcg tggtactaac aggcttcgtg
     2641 ctcccgcgat tcgagcggtt cttcgtttca ttcaacgcca agctcccgct cccgactcgc
     2701 atccttcttg ggatcgcggg tttcatgggc cgatggtggt ggctgatcgt tgctggactc
     2761 gtcgtgacgg ttgtcggcct ggtactcatc tcccgcacca cgcgaggtag ggctgccaga
     2821 gacagggttc tactgcggct tcctgcgata ggcgatgtag tcaggttcgc cgtcatcgag
     2881 agagtggcca ggatcctcgc ttcgacgatc cgggcgggtg ttcctctgcc ggaggcgatg
     2941 tcggttaccg cagagggcgc gcataacgtc gtatatcagc gtgcattggg ggaagtccga
     3001 gaggccatga tgcgcggtga ggggatagca cggccgcttg cagatacgaa gctcttcccc
     3061 acctcggtcc ttcagatggt caaggtggga gaacacaccg
//
LOCUS       DATDOZ010000207         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_695738, whole genome shotgun sequence.
ACCESSION   DATDOZ010000207 DATDOZ010000000
VERSION     DATDOZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695738"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VI541_04155"
     CDS             complement(<1..519)
                     /locus_tag="VI541_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF70128.1"
                     /translation="MSFEITVLGCSGTYPVPGNAATGFLLRSDDSTIWIDAGTGTFAN
                     LQRHVDYRTLDAVLLSHLHLDHILDLFPLYYALRYSVEKLAPTMLQVFAPEEAESSLG
                     AFVSKDRDPFGDYLVFRPIADGSIGGFDFSFVRARHPVPTFAMRIDRDGASFGYTADT
                     APSDAVTTLMKGV"
     gene            complement(509..1339)
                     /gene="murI"
                     /locus_tag="VI541_04160"
     CDS             complement(509..1339)
                     /gene="murI"
                     /locus_tag="VI541_04160"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLF70129.1"
                     /translation="MSKADKPIAIFDSGVGGLTVVRAIEELLPQENLIYFGDTARFPY
                     GPRAVDQVKAFALEVMDFMASQDVKMLVIACNSMTAAAYSEAIERYDLPVIGVIEPAV
                     RAAVRATHNRKVGVLATKATIATGEYERALGRTRQKVEIHSQVCGGFVEHVEAGTTYS
                     EELFELAREYLTPLMALGVDTMILGCTHYPLLRGVLHAVTKGEVELISSADEVAKDVY
                     SALVEKDLLRRVAIIGERRFMVSGDPASFAAVGSRFLAGLSRVESRPWDLTRATTDVI
                     "
     gene            complement(1336..2268)
                     /locus_tag="VI541_04165"
     CDS             complement(1336..2268)
                     /locus_tag="VI541_04165"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HLF70130.1"
                     /translation="MRSDDIEALVGETPLVGLTRLSSKPDVRIWAKLEGQNPTGSVKD
                     RIARLMIEDAISSGELNQDRILIEPTSGNTGISLAFFARRRGLRLVCVMPETASAERA
                     GLIRAFGAEVVWSPGPQGSNGAIEMARKMAVDDPAYLMLYQYGNDANPRAHYETTGPE
                     ILRDLPQITHFVAGLGTGGTLTGVGRYLKEQKAEIKIIAAEPEQGEMVYGLRSLEDGF
                     VPPVFDPSVLDSKIKVRASDSVFWTQALATQEGVFAGISSGATLHVARRIAERIDGGD
                     IVCLFADGGWKYLSTEIWTKQVEEAARDVEYMPW"
     gene            complement(2321..2740)
                     /locus_tag="VI541_04170"
     CDS             complement(2321..2740)
                     /locus_tag="VI541_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HLF70131.1"
                     /translation="MKLHIDPSVHDAMLEHCLAGRPNEACGFLGGTGGQATLIYPLRN
                     AAASPVFYEPDGREMLRALDDLDRQGSDLLAIFHSHVATAPWPSPTDVREAHHPGVVY
                     LIVSLMSEAPEVKGFLIEKADWRDKEGEIVEVDLVVS"
     gene            complement(2737..>3461)
                     /gene="selD"
                     /locus_tag="VI541_04175"
     CDS             complement(2737..>3461)
                     /gene="selD"
                     /locus_tag="VI541_04175"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HLF70132.1"
                     /translation="PKDLDPAALASVLEGGLDKSREVGLSIIGGHTVDDPEPKYGMAV
                     TGLVDIERIITSSAARPGMQLILTKRIGTGVISTALKAGRAPEALVAEATSSMKSLNK
                     DAARVMRDHAVAAATDVTGFGLLGHLHNLLRLSGVSARLQASKVHFFEGVAELVGQGS
                     VSGGTRRNRNYFERWVTFDNAVDPLTQTLLFDAQTSGGMLMAVDPQRVELTLAALSAA
                     GVPAAAIGEVLVGDGNTIEVAG"
BASE COUNT          683 a         1103 c          996 g          679 t
ORIGIN      
        1 gactcctttc atcagtgtcg tcaccgcgtc actcggggct gtgtcggcgg tatatccaaa
       61 gcttgctcca tctcgatcta tccgcatcgc gaaagtggga accggatggc gtgctctcac
      121 gaaactgaag tcgaagcccc ctatggaacc atccgcgatg ggtctgaaga cgaggtagtc
      181 gccgaacgga tcacgatcct tcgagacgaa tgctcccaaa ctcgattcgg cctcctcggg
      241 cgcgaatacc tgcagcatgg tcggcgccaa cttctcgacc gaataccgca gggcgtaata
      301 caggggaaag agatccagga tgtggtcaag gtgcagatgc gaaagcagga ccgcatctaa
      361 ggttcgatag tccacatggc gctgaagatt tgcaaacgtg ccggtgccag catcgatcca
      421 gatcgtcgaa tcatctgaac gcaagaggaa tcccgtcgcc gcgttaccgg ggacgggata
      481 ggtgccgctg cacccgagca cggttatctc aaatgacatc tgtcgtagcc ctcgtgagat
      541 cccagggcct gctctcgacc cgagacagtc cagcaaggaa tcgagagccc acagcagcga
      601 aactcgcggg gtccccggaa accatgaacc ggcgctctcc gatgatcgca acccttcgca
      661 gcagatcctt ctcgacgagg gcagagtaga cgtccttggc tacttcatcg gccgatgaga
      721 tcagctcgac ctcgcctttg gtgaccgcgt ggagcacgcc ccgcaggagt gggtagtgcg
      781 tgcaacccaa gatcatggtg tcgactccca gagccatgag tggcgtcagg tattcgcgag
      841 cgagctcgaa aagctcctcc gagtaggtcg tacccgcctc gacgtgctcg acgaatccgc
      901 cgcatacctg cgagtggatc tctaccttct gccgcgtccg gccaagcgcg cgctcgtatt
      961 ctcccgtggc gatcgtagct ttcgtggcca gtactcctac cttgcggttg tgcgttgcgc
     1021 gcaccgcggc acggaccgcg ggttctatca ctccgatgac cggcagatcg tagcgctcga
     1081 tggcttcgga gtatgcagcc gccgtcatgg agttacaggc gatgaccaac atcttcacat
     1141 cctgcgacgc catgaagtcc atgacctcca gagcgaaggc tttcacctgg tcaacggctc
     1201 gtggcccgta ggggaatcgc gcggtgtcgc cgaaatagat gaggttctct tgcgggagaa
     1261 gttcctcgat cgctctgacg accgtcaggc ccccgacccc cgaatcgaat atcgctatcg
     1321 gcttatctgc tttgctcacc acggcatgta ctccacgtcg cgcgctgctt cttcgacctg
     1381 ctttgtccat atctcggtag aaagatactt ccaacctcca tcggcgaaga ggcagacgat
     1441 gtccccgccg tcgatacgct cggcgatcct tctggccaca tgcagagttg ccccggaaga
     1501 gatgccagcg aagacccctt cttgggtcgc cagagcttgc gtccagaaca ctgaatcgga
     1561 agcgcgcacc ttgatcttgg agtcgaggac cgacgggtcg aacaccggcg gcacgaaccc
     1621 atcttcaagc gagcggagcc catagaccat ctcgccctgc tcgggctctg cagcgatgat
     1681 cttgatctcg gccttctgtt ccttcagata gcgacctact ccggtgagcg tcccgcccgt
     1741 accaaggcct gccacgaagt gggtgatctg tggcaggtca cgtaggatct cggggcccgt
     1801 cgtttcgtag tgagctctcg gattggcgtc gttcccgtat tgatacagca tgagataggc
     1861 ggggtcgtcg accgccatct tcctcgccat ctcgatggcg ccgttcgaac cctgggggcc
     1921 tggtgaccag acgacctcgg cgccgaaagc ccttatcagc cccgcgcgtt cagccgatgc
     1981 cgtctctggc atcacgcaga cgagacgcaa accgcgccgt cgcgcaaaga aggcgagtga
     2041 gatgcctgtg tttccggatg ttggttcgat caggatgcgg tcctggttca gttcccccga
     2101 cgagatcgcg tcctcgatca tcagccgggc gatccggtct ttcacggacc cggttgggtt
     2161 ctgaccttcg agtttcgccc atatccgcac atcgggcttg gatgaaagtc gagtcagacc
     2221 gacaagaggc gtctctccta ccaacgcctc gatgtcatcg gaacgcacgg atcagttccc
     2281 tcgacagacg gccggggttt ctacttcgac aggcatcgac tcaagatacc accaggtcta
     2341 cctcgactat ctcgccttcc ttgtcgcgcc aatcggcctt ctcgatgagg aatcccttga
     2401 cttcgggagc ctcactcatc agagagacga tgaggtagac aaccccggga tgatgcgctt
     2461 cccgaacgtc ggtcggcgag ggccacgggg ccgtggctac atgtgaatga aatatcgcca
     2521 gcaggtccga tccctgtcgg tccagatcat cgagggccct caacatctcc ctgccatcgg
     2581 gctcgtagaa gacgggggac gcagcggcgt tgcggagcgg gtagatcaac gtcgcctgcc
     2641 ccccggtccc cccgaggaac ccgcaggcct cgttcggcct gcccgccaaa cagtgctcga
     2701 gcatcgcgtc atgcaccgaa gggtcgatgt gcagtttcat ccagcaacct cgatcgtgtt
     2761 gccgtcccca acaagaacct cgccgatcgc tgcagcggga accccagcgg ccgagagggc
     2821 cgccaaggtc agctccacgc gctgaggatc cacggccatc aacatcccac ccgaggtctg
     2881 cgcatcgaag aggagagttt gagttaacgg atcaaccgcg ttgtcgaagg ttacccatcg
     2941 ttcgaagtag ttgcggttcc ggcgcgtgcc ccctgagacc gatccctgcc cgacgagttc
     3001 ggcgacccct tcgaagaagt ggaccttcga tgcttgaagc cgtgcggaaa ctccacttag
     3061 tctcagcagg ttgtggaggt gtccaaggag gccgaatccg gtgacatcgg tcgccgcagc
     3121 caccgcatga tccctcatca ctcgagctgc atccttgttc aacgacttca tcgacgacgt
     3181 ggcctctgcc accagggcct cgggggcgcg gcccgccttc aacgctgtcg agatcacgcc
     3241 cgttccgatc cgtttggtga ggatgagctg catccccgga cgcgccgctg acgaggtgat
     3301 gatccgctcg atatccacca aacccgtcac agccatcccg tacttgggtt cggggtcatc
     3361 gaccgtatgg cccccgatga tagaaagacc aacctctcgg ctcttgtcga gtcctccttc
     3421 gaggacagac gccagagcgg cggggtcgag gtccttgggc c
//
LOCUS       DATDOZ010000208         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3565728, whole genome shotgun sequence.
ACCESSION   DATDOZ010000208 DATDOZ010000000
VERSION     DATDOZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565728"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VI541_04180"
     CDS             complement(<1..832)
                     /locus_tag="VI541_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLF70133.1"
                     /translation="MPHTLKDVLPVTATVNDEGHLVIGGLDTTALAEEFGTPLFVFCE
                     ETFRTRARQFRSAFPDSDIYYASKAFLSLAMCDLIEQEGLSLDVASGGELHTALAAGF
                     PAERMILHGNNKNDGDILRAVEAGVGCIAIDSLEEVGRVAAAAADIGCTQKVVVRVTP
                     GVEAHTHHYIQTGQEDSKFGLSLAGGLAHEAMVLAHDDPHLELVGIHAHIGSNIFSYD
                     PFGKTVEILFAFLQEVRERLGITLEEVDLGGGVGIPYVAGDYPARLDLMSQLVLETVA
                     R"
     gene            complement(835..>2054)
                     /gene="argS"
                     /locus_tag="VI541_04185"
     CDS             complement(835..>2054)
                     /gene="argS"
                     /locus_tag="VI541_04185"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLF70134.1"
                     /translation="YGDALANLLAAIGYRVSREFLVNDYGKQVMGFARSLEARYRQAL
                     GHDAEVPADGYRGDYLIEMGKELAASEGMGLIGRLPEIRHWGITHVLASHRATLEKFG
                     VTFDNWFSETYLHTTGKVDAAIARLTELGYTYESEGAIWFKASESGASRDQVLIRSGD
                     PTPTYLAADVAYMLDKLERGFERILYVWGADHHGNSETLMAAARVLGIEDRVEIIIYQ
                     LVNFLSKGEPVRMSKRTGDLVTMDELIDEVGVDAARYTLLTRSTDSTIDFDLETVKAE
                     SQDNPVYYVQYQYARISSILRYAVERSIALRPAKEVDLGRLTHPSETTLIQKLSEYPE
                     LIESSAKLRAPFRITNYMQSLGAAFSAFYRDCRAITDDTELTQARLALASAARQVLSN
                     AFAVLGVSAPERM"
BASE COUNT          375 a          609 c          674 g          396 t
ORIGIN      
        1 cgcgagccac ggtttctaga accagctgtg acatcagatc gaggcgggcg gggtaatcgc
       61 ctgcgacgta ggggatgcca actccgcccc cgaggtcgac ctcctcaagc gtgattccga
      121 gtcgctcacg aacctcctga aggaacgcga acaagatctc cacggtcttg ccgaatgggt
      181 cgtaagagaa gatgttcgag ccgatgtgcg cgtggatgcc gacgagttcg aggtgggggt
      241 cgtcgtgcgc caagaccatg gcctcgtggg cgaggccccc tgccagcgat agaccgaact
      301 tgctgtcctc ctgaccggtc tggatgtagt ggtgtgtatg cgcttcgacc ccgggcgtca
      361 cccggaccac gaccttctga gtgcagccga tgtcggcggc ggcggcagcg actcggccta
      421 cttcttcgag gctgtcgatg gcgatgcaac caaccccagc ttccaccgcg cgaaggatgt
      481 cgccgtcgtt cttgttgttg ccatgcagga tcatccgttc cgccgggaac cctgcggcga
      541 gggcagtgtg cagctctccc cccgatgcga catcgaggct cagcccttcc tgttcgatga
      601 ggtcgcacat ggcaaggctg aggaatgcct tcgatgcgta gtagatgtcc gagtctggga
      661 aggcagagcg gaactgccgt gcgcgcgttc gaaaggtctc ttcgcagaac acgaagaggg
      721 gcgtgccgaa ctcctcggcc agtgcggttg tgtctaggcc cccgatcacc agatggcctt
      781 catcgttgac ggtggcagtg accgggagaa cgtccttgag ggtgtgtggc atcgctacat
      841 gcgttcgggg gccgagaccc cgaggacggc aaaggcgttg gacagcacct gacgggccgc
      901 ggacgcgagc gccaggcgag cctgggtgag ctcggtgtca tcggtgatcg cgcggcagtc
      961 tcggtagaag gcgctgaacg ctgcaccgag ggactgcatg tagttggtga tgcggaaggg
     1021 ggcccggagc ttcgccgagg actcgatcag ctcgggatac tccgagagct tctggatgag
     1081 ggtcgtttcg gagggatgag tgagcctgcc caggtcgacc tccttcgctg gcctcaacgc
     1141 gatcgatcgc tcgaccgcgt agcgaagtat cgacgagatg cgggcgtact gatactggac
     1201 gtagtagacg gggttgtcct gcgactcggc cttcactgtt tcgaggtcga agtcgatcgt
     1261 tgaatcggtg gaccgcgtca ggagcgtgta ccgcgcagcg tccacgccga cctcgtcgat
     1321 cagttcatcc atcgtcacga gatcccctgt gcgtttcgac atccgtaccg gctcaccctt
     1381 cgaaaggaag ttaacgagct gatagatgat gatctcgacg cggtcctcga taccgagcac
     1441 ccgtgccgcg gccatcaggg tctcggagtt gccgtgatgg tccgcacccc aaacgtagag
     1501 gatcctctcg aacccccttt cgagtttgtc gagcatgtaa gcgacatctg cggcgaggta
     1561 cgtgggggtt gggtcacccg agcggatgag cacctgatct ctcgacgccc ccgactcgga
     1621 ggctttgaac cagatcgcgc cctcggactc gtaggtgtaa ccaagttcag tgagccgagc
     1681 gatggcagcg tcgaccttgc cggttgtatg caggtaggtc tcggagaacc agttgtcgaa
     1741 tgtgaccccg aacttctcga gcgtagcgcg gtgcgaggcg agcacatgcg tgatacccca
     1801 atgtctgatc tctggcagcc gaccgatgag ccccatcccc tcagaggcgg cgagttcttt
     1861 ccccatctcg atgaggtagt caccgcggta gccgtcggct gggacctcgg catcatgccc
     1921 gagtgcctgg cggtagcgag cctcgagcga gcgtgcgaac cccatcacct gtttgccgta
     1981 gtcgttcacg aggaactcgc gcgagacgcg gtacccaatc gcggcgagca ggttcgccag
     2041 cgcgtcaccg tatg
//
LOCUS       DATDOZ010000209         3968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2529421, whole genome shotgun sequence.
ACCESSION   DATDOZ010000209 DATDOZ010000000
VERSION     DATDOZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529421"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VI541_04190"
     CDS             <1..390
                     /locus_tag="VI541_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF70135.1"
                     /translation="LTLSIQLIASWGRYERGSMLEVMGADYMRTARAKGLSEVRVVLR
                     HGLRNALIPFVTVVALDVGLLFGGLIITEQIFSWPGMGSLLLGALSVGDYPVVLAALM
                     VTAGFVVLFNLIADVLYGVLDPRIRYD"
     gene            383..1333
                     /locus_tag="VI541_04195"
     CDS             383..1333
                     /locus_tag="VI541_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF70136.1"
                     /translation="MTEVPVTTEQEPLEFAAASRTQFQMTWSRFRQHKMAMVSMFVLA
                     ILYLSAIFAPLIAPYDPDTGCPSHVKKHCAALNSEVLADSRQPPSRHHPMGTDHLGRD
                     ELSRVLYGGRISLAVGLAVAIVAGLVGTAVGAVAGYYGGWIDSLLMRITELFLIVPAL
                     LILLVISTLLGGSVFDIILAISLLAWMPLARIVRGVFLSVKEKEFIEAARASGAKSSR
                     IIWRHMLPNTVGPIIVYATLGVAAAILTESVLSFLGFGIKPPTSTWGNMIFEGKDAMI
                     TQPWLMLFPGLMIFLTVLSVNFLGDGLRDALDPTGQRIRA"
     gene            1330..2337
                     /locus_tag="VI541_04200"
     CDS             1330..2337
                     /locus_tag="VI541_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF70137.1"
                     /translation="MTEALLSVRNLSTRFDTDDGVVRAVDEVSFDVMPREIFGIVGES
                     GSGKSVTALSILGLLPKPAGRVAGGQVLYKGSDLLAMDPGELRKLRGNRITMIFQDPL
                     TALNPVFTVGNQIAEVFRIHQGISKQAAARKSIDMLRLVGIPKPEQRADDYPHQFSGG
                     MRQRAMIAMAVALDPDLLIADEPTTALDVTIQAQIIDVLLQVREECSMAIMLITHDLG
                     VVAGVAERVMVMYAGKVAEIATVDEIFTSPKHPYTWGLMTSIARLDEARKARLIPIRG
                     APPSVMNEWTSCPFSPRCDYRQDVCDEKYPSLISVSDAHDAACHFARDSGWAPRVGAQ
                     P"
     gene            2334..3383
                     /locus_tag="VI541_04205"
     CDS             2334..3383
                     /locus_tag="VI541_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLF70138.1"
                     /translation="MSPQILRVEGLVKRFPIRKGFLRRVVGSVHAVDGVDLHIDAGET
                     LGVVGESGCGKSTLARCILRLIEPTAGRVFFEDQEVTTLEPEELRLVRRQMQIVFQDP
                     YASLNPRMTVRDIIAEPMVIHRLHAGRHDKRVADLLERVGLQGAHGNRYPHEFSGGQR
                     QRIGIARALALEPRLIVCDEPVSALDVSIQAQILNLLEDLQSDLGLTYMFIAHDLSVV
                     RHISDRVVVMYLGKIVESADRLQLYERPMHPYSVALLSAIPIPDPVKERARHRIILRG
                     DVPNPARPPSGCRFHTRCPLAQEIAKNNDVAVVRIGEKHHVPVICHEEEPQMQDVDAM
                     HSVACHFATAVTSRS"
     gene            3380..>3968
                     /locus_tag="VI541_04210"
     CDS             3380..>3968
                     /locus_tag="VI541_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF70139.1"
                     /translation="MRLALSRCLAMTTLLTVFAACSGPTKTAKVVTPAINLRVAITNV
                     DSLDPITAVGEGSQLILRTACDGLVALDQEGVVRPALAESWTVSQGGRKLTLRLRGKT
                     RFLDGTDVDAEAVADALNRVVRLESASPWANLLSSVQGFEDVRSGAQARLSGVTAPDN
                     RTVAITTSQPFSDLVANLAHTALIPTSPRDDGAATP"
BASE COUNT          767 a         1161 c         1213 g          827 t
ORIGIN      
        1 ctgacccttt ccatccagtt gatcgccagt tggggccgat acgaacgagg aagcatgctc
       61 gaggtcatgg gggcggacta catgcgaacc gctcgagcga agggactgag tgaagtgcgc
      121 gttgtcctgc gtcacggtct ccggaacgcc ctgatcccat tcgtcacggt ggtggctctc
      181 gatgtaggtc tccttttcgg aggcttgatc atcacggagc agatcttctc gtggccgggt
      241 atggggtctc tacttctggg ggccctgagc gttggcgact acccggtggt tctggcggcc
      301 ctgatggtga cggccgggtt cgtggtgctc ttcaatctca tcgccgacgt gctctacgga
      361 gttctcgacc cgaggatccg ttatgactga ggtgccagtg accacggaac aggagccgct
      421 ggagttcgcg gcagctagcc gcacacagtt ccagatgaca tggtcaagat tccgccagca
      481 caagatggcg atggtgagca tgttcgtact ggcgatcctc tatctctctg cgatcttcgc
      541 cccactgatc gctccctacg atccagacac ggggtgcccg agtcacgtca agaagcactg
      601 tgcagctctg aatagcgagg tgctggcaga ctctcggcaa ccgcccagca gacatcatcc
      661 gatgggaacg gaccatctcg gtagagatga gctcagccgt gtcctctatg ggggccgtat
      721 atcactggcg gttgggctgg ccgtcgctat cgtcgcgggg ctggttggca ccgcggttgg
      781 ggcggtcgcc ggatactacg gaggctggat cgactcgctg ctcatgcgca taacggagct
      841 cttcttgatc gttcctgcgc ttctgatact tctagtcata tcgactttgc tgggaggctc
      901 ggtgttcgac atcatcctcg cgatctctct gctggcctgg atgcctttgg cccgtatcgt
      961 gcgcggtgtc tttctctccg taaaggagaa ggagttcata gaggccgctc gagcgtcggg
     1021 agccaagtcg agtcggatca tctggcgcca catgctcccg aacaccgtcg gtccgatcat
     1081 cgtctacgcg accctcgggg tagccgcggc catcctcacc gaatcggttc tctcgttcct
     1141 cgggttcggg ataaaacctc cgacctcgac ctggggaaac atgatcttcg aagggaagga
     1201 cgccatgatc acccagcctt ggctcatgct cttccccggt ctgatgatct ttctgactgt
     1261 tctcagcgtg aacttcctcg gggacggtct gcgtgatgct ctcgatccca cgggccagcg
     1321 gatcagggca tgacagaagc tcttctttcg gtgcggaacc tgtcgacacg cttcgatacc
     1381 gacgacggcg tggtgagggc agtcgatgag gtctccttcg acgtcatgcc cagagagata
     1441 ttcgggatcg tcggcgaatc ggggtcaggt aagagtgtca cggcactgtc catcctcggg
     1501 ctccttccca agccggcagg gcgcgtggcc ggcggacagg ttctgtacaa ggggtcggac
     1561 cttttggcga tggaccccgg agaactgcga aaactaagag ggaaccggat aacgatgatc
     1621 ttccaggacc cccttacggc cttgaacccc gttttcacag tcggaaacca gatcgcagag
     1681 gtctttcgga tccaccaagg catctccaag caggccgcgg ccagaaaatc gatcgatatg
     1741 ctgaggctcg ttggcatccc taagccggag cagcgagccg acgactatcc acatcaattc
     1801 tcggggggga tgagacagag agcgatgata gcgatggccg tcgcgctcga ccctgacctc
     1861 ttgatcgccg atgagccgac gacggccctc gatgtgacca tccaagctca gataatcgat
     1921 gtccttctgc aggttcggga ggagtgcagc atggcgatca tgctcataac ccacgacctc
     1981 ggagtcgtag ctggcgtggc cgagagagtg atggtcatgt acgcaggcaa ggtcgcagag
     2041 atcgctacgg tcgatgagat cttcacgtct cccaaacacc catacacgtg gggcttgatg
     2101 acttcgatag ctcggctcga tgaggcgcgc aaggcgcgac tcatcccgat caggggggcg
     2161 ccgccatctg tcatgaatga gtggaccagc tgtccgttct cgccgagatg cgactatcgc
     2221 caggacgtct gtgacgagaa gtatccgtcg ttgattagcg tgtcggatgc ccacgacgct
     2281 gcatgtcact tcgctcgtga tagcggctgg gcccccaggg tgggggccca gccttgagtc
     2341 cacagatact tcgcgtcgag ggactagtaa agcgattccc gatccggaag gggttccttc
     2401 ggcgggtggt cgggtccgtc catgcggttg atggtgtgga tctgcacatc gacgccggtg
     2461 agaccttggg ggtcgtgggc gagtcgggtt gcggaaagtc gacactagcc agatgcatcc
     2521 tgcgtctcat agagcctacg gccgggcggg tgttcttcga ggatcaagag gtcacgacgc
     2581 ttgaaccaga ggaattgcgc ttggtgcggc gacagatgca gatcgtattc caggatccct
     2641 acgcctcact caatccaagg atgacggtgc gcgacatcat cgctgaaccg atggtgatcc
     2701 atcgtcttca cgctggccgt cacgataaaa gggtcgccga tctgctcgag agggtcggcc
     2761 tccagggcgc tcacggcaat cgatatccac atgagttctc tggtggccag cggcagagga
     2821 taggtatcgc ccgggccttg gcgctcgaac cgcgattgat agtttgcgat gaaccggtgt
     2881 cggcactgga cgtttctatc caggcgcaga tcctcaatct gctcgaagac cttcagtccg
     2941 atctcggact cacgtacatg ttcatcgcgc acgacctttc tgtcgtgcga cacataagcg
     3001 atcgagtggt ggtgatgtat ctgggcaaga tcgtggagtc ggccgatcgc cttcagctct
     3061 acgagagacc catgcacccg tactcggttg cccttctctc ggctatccct atcccggacc
     3121 cggtgaagga gagggcacgc catcgcatca tccttagggg tgacgtgccc aatcctgcaa
     3181 ggcccccatc cggctgcagg ttccatacga ggtgcccact ggcccaggag atagcaaaga
     3241 acaacgatgt ggcggtggtt aggatcggag agaaacacca tgtcccggtg atctgccacg
     3301 aggaggaacc tcagatgcag gatgtcgatg ccatgcactc cgtggcctgt cacttcgcaa
     3361 ctgcagtcac atcgaggtct tgaggctggc cctctcgcgc tgcttggcga tgacaacgct
     3421 gctgacggtg ttcgcagcct gttcggggcc gaccaaaacg gccaaggtcg tcacgccagc
     3481 catcaacctt cgtgtcgcga tcaccaacgt cgattcgctc gatccgataa ccgctgtcgg
     3541 ggagggatct cagctgatcc tacgaaccgc atgcgatggt cttgtcgcgc tcgaccaaga
     3601 aggcgttgta agaccagcgt tggctgagtc ctggaccgtc tcgcaggggg gccggaagct
     3661 cacccttcga cttcgaggga agacacgatt tctcgatggc acagacgtgg atgccgaggc
     3721 agtcgctgat gcgctgaacc gcgtggtaag actcgagtcg gcatcgccgt gggcaaacct
     3781 gctctcatcg gtccaggggt tcgaggatgt cagatcggga gcgcaggcgc gtctcagcgg
     3841 tgtcacggcc cccgacaacc gcacagtcgc cataacaaca tcgcaaccat tcagcgatct
     3901 cgtggcgaat cttgctcaca ctgccctgat ccctacctcg ccgcgcgacg acggggccgc
     3961 aacacctc
//
LOCUS       DATDOZ010000210         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1753962, whole genome shotgun sequence.
ACCESSION   DATDOZ010000210 DATDOZ010000000
VERSION     DATDOZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753962"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VI541_04215"
     CDS             complement(<1..306)
                     /locus_tag="VI541_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70140.1"
                     /translation="MKMRSRTLVLLRRSSNPPTLSEDELEALQRGHLAFLVSMREAGH
                     MSEAGPFSEQEDQGLRGLCLYATPIEQTRELVRGDPMVKAGWLRAEVFTWWTPDPGPG
                     "
     gene            328..1278
                     /locus_tag="VI541_04220"
     CDS             328..1278
                     /locus_tag="VI541_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF70141.1"
                     /translation="MEPPELIANLTDVSRSRAMEVEPLGYYIKEAPMCIRVRLTRTVL
                     VAALIGSAVIAVTPARADCAPAHFYHCSPGQEAPFANDPNCTSPGKSPVVLVHGTYTD
                     QTMSWNLFGPALHNAGYCIWSLDYGDRATASMEVSIHQITDFVVNTVLPTSSTEKVSL
                     IGHSQGGLQIRHMLRHDDTIPVDDAISLAGSHYGTTVFAAPWAAQYGGCPACGEQVAG
                     STWMTGHMNAALEDATPGEFTSYTQIETKYDEVVTPYTNTFLPAESGVVTNITLQDKC
                     PADSYEHVGIIYDANALGWVLNALATTGPADPTFVPTGCQ"
     gene            1396..2613
                     /locus_tag="VI541_04225"
     CDS             1396..2613
                     /locus_tag="VI541_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF70142.1"
                     /translation="MDFAFTPKVEDLRNRLLDFMGSHIYPAETTYQEQMAKSGDPHYH
                     PPIIEDLKDEAKQRGLWNLFLPDARWGAGLNNLEYAPLAEITGRSYLAPEAINCSAPD
                     TGNMEILAEFGTPEQQERWLQPLLEGEIRSCFGMTEPEVASSDASNIQSRIERDGDEY
                     VINGRKWWTSGAGSDRCKVMIFMGVTNPDADAYQRQSMILVPMDAPGLNIERTLPVFG
                     YEHREGHADITFENVRVPATNILGAEGAGFLIAQARLGPGRIHHCMRAIGMAERAFDL
                     MCKRANERVAFGKPLAAQGVVRDQVAESRIEIEQVRLLVLKAAWLMDTVGKDQARIEI
                     SAIKVAAPRVALNVIDRAIQIHGGAGVSDDFPLAAMYAGARTLRIADGPDEVHKMVIA
                     RRELRRYATDPNR"
     gene            3102..>4107
                     /locus_tag="VI541_04230"
     CDS             3102..>4107
                     /locus_tag="VI541_04230"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HLF70143.1"
                     /translation="MPGPFVPTFGPPGSLEIVLPPKARSGPPGERLIIAGSFTRPCAL
                     FLLHFDGTFITDAKPSANGYSFSDARFKIPYDARPGPHTISAHCNDANVSAAGLSAIG
                     NDPVGTLPFTVDPSAPDVGSDHRSGFARSVPDPTTVVNGIKHNRELVGQNILLALILL
                     LMITFPAELFNSTLEENYEEVQGWFKRLRRVGRGLGRHLRKLPSMVSVALFAIVGGLV
                     YGFLDPAFGADRDSLINVAGMAAGILAITLTFDLTAVRSLRRRYPASTRLRILPGTMV
                     VAALCVLLSRVVDFSPGYFYGVIAGWVISAETSHREEGRATAIGSTAMMVMSVGAWLL
                     W"
BASE COUNT          816 a         1387 c         1146 g          758 t
ORIGIN      
        1 cccgggccct gggtccgggg tccaccatgt gaagacttcg gccctcagcc aaccagcctt
       61 gaccataggg tcccctctca cgagctcgcg ggtttgctcg atcggggtcg cgtacaggca
      121 cagcccccgc aacccctggt cctcttgctc cgaaaaggga ccagcctcgg acatgtgtcc
      181 cgcttcccgc atggaaacga ggaaggccag atgccccctc tgcagtgcct cgagctcatc
      241 ttcactcagc gtcggagggt tcgacgacct ccggaggagc acgagagtcc tcgacctcat
      301 cttcatccga tcaacctcac tgtcgtgatg gagccgcctg aactgatcgc caacctaacc
      361 gacgtgagca ggagtcgcgc gatggaggta gaaccattgg ggtactacat caaggaggct
      421 ccaatgtgta tcagagtcag actgacccgc accgtcctcg tggccgcact cataggcagt
      481 gcagtgatcg ctgtcacccc tgcccgcgct gactgcgcac ccgctcattt ctatcactgc
      541 tctccaggcc aagaggctcc attcgccaac gaccctaact gcacctcgcc ggggaagtca
      601 ccggtcgttc tcgtacatgg aacctacacg gaccagacca tgtcgtggaa cctgttcggg
      661 ccagccctgc acaacgcggg ttactgcatc tggtcgctcg actacggcga tcgggccact
      721 gccagcatgg aggtttccat ccaccagatc acagacttcg tcgtgaacac tgtccttccc
      781 acatcgagta cggagaaggt ctcactcatt ggccactcgc aggggggact gcagatccgc
      841 cacatgctcc gacatgacga caccatcccg gtcgatgacg cgatctcact cgctggctcc
      901 cactacggga ccacagtgtt cgcagccccc tgggcggcgc agtatggcgg gtgtccagca
      961 tgtggtgaac aggtcgcggg ttcgacttgg atgactggtc acatgaacgc cgctctagag
     1021 gatgcgaccc ccggcgagtt cacctcgtac acgcagatcg agaccaagta cgacgaggtc
     1081 gtcacgcctt acaccaacac cttcctccca gccgaatcgg gtgtcgtaac caacatcacc
     1141 ctgcaggaca agtgccctgc cgacagctat gaacacgttg ggatcatcta cgacgccaat
     1201 gctctcggtt gggtactcaa cgcgctcgca acgaccgggc ccgctgaccc gaccttcgtc
     1261 ccaacaggct gccaatagag cgcggcgtcg acttcttttg gtcactggga acctatgcgg
     1321 tctctggcga cctaaagaag tgcaggagcg gaaaccaata gagttctgag cgaccatccc
     1381 gataggagct cagacatgga cttcgcattc actccgaaag tagaggatct tcgcaatcgt
     1441 ctcctcgatt tcatggggag ccacatctac cccgccgaaa ccacctatca ggagcagatg
     1501 gccaagtcgg gtgaccctca ctaccacccg ccgatcatcg aggacctcaa ggacgaagcg
     1561 aaacaaaggg gcctttggaa cctgttcctg ccagacgccc gatggggcgc agggctcaac
     1621 aacctcgagt acgcaccgct ggccgagatc accggccgca gttacctggc ccccgaagcc
     1681 atcaactgct cggcccccga caccggcaac atggagatac tcgccgagtt cggaactccc
     1741 gagcagcagg aacgctggct ccaaccactc ctcgaaggag agatccgttc ttgcttcggc
     1801 atgaccgaac ccgaggtcgc cagttcagac gcctccaaca tccagagtcg catcgagcgt
     1861 gacggcgacg agtatgtgat caacggccgc aaatggtgga catcgggagc cggcagtgat
     1921 cgatgcaagg tcatgatctt catgggggtc acgaaccccg acgccgacgc ataccaacga
     1981 cagagcatga tcctcgtgcc catggacgcc ccggggctca acatagaacg cactctgccg
     2041 gtcttcggct acgaacatcg cgagggacac gctgacatca cgttcgagaa cgtcagagta
     2101 ccggcgacga acatcctggg ggccgagggc gcgggctttc tcatcgcgca ggccaggctc
     2161 gggcccggtc gtatccacca ctgcatgcgc gcgatcggga tggccgagag ggccttcgac
     2221 ctgatgtgca agagggcgaa cgagcgcgtc gcgttcggta agccacttgc agctcaggga
     2281 gtcgttcgcg accaggtcgc cgaatcacgc atcgagatcg agcaggtgcg actgctggtc
     2341 ctgaaagcgg cctggctgat ggataccgtt ggcaaggatc aggcgcgcat cgagatctcg
     2401 gccatcaagg tcgcagcccc cagggtcgca ttgaacgtga tcgaccgcgc gatccagata
     2461 cacggcggtg ccggggtctc cgacgacttc ccactggccg cgatgtacgc gggggcccgg
     2521 accctgcgca tcgcagatgg tcctgatgaa gtgcacaaga tggtgatcgc ccgacgggag
     2581 ctgcgccgct acgcaaccga tccgaatcga tgatccgact tgcaatcatc gcatcatcga
     2641 tcgttctgat gctctgccta tcgttcatcc cttcctcggg acaagcggcg aaccaacccc
     2701 cttcggtcag tccgatcgac tctcaggcgt cctccgctgc ccccataacg acatatcggt
     2761 tctcagcaac ggatcctgat gaagacccac tcaggttcac ctggacgaag gaccaacagg
     2821 actcgtgcgg gccattcacc tatagcggtg actcggcgac ttgggactac tcgaactgtg
     2881 cagccggtcc ccatcccggc ctgataacag ccgtcgtaac agatggcttc ttcgtctgca
     2941 ctggcaccta cgctggtgga gcaaacaccg ggacgggtga tccaccttcc tgtcgcccac
     3001 tgactgcacc aagcccatct ccatctccat ctcctcggcc aacgaggtcg ccctcgccct
     3061 cgccctcgcc ttcagagtct ccaattccga cggtttccgt tgtgcccggt ccattcgtcc
     3121 cgaccttcgg cccccccggt tctctggaga tagtcctgcc cccgaaagca cgatccgggc
     3181 cccccggaga gcggctcatc atcgctggct cattcacgcg cccttgcgcg ctcttcttgc
     3241 tgcacttcga cggaaccttc atcacagacg cgaaaccgag cgccaacggt tactccttca
     3301 gtgacgcccg cttcaagatc ccctacgatg cgcggccggg cccccacacc atctcggctc
     3361 attgcaacga cgccaatgtg agcgctgcgg gactaagcgc gattggcaac gatccggtgg
     3421 ggacactccc gttcaccgtc gacccgtcgg cccccgatgt ggggtcagac caccgctccg
     3481 gcttcgcacg atcggtccca gacccgacga ccgtcgtcaa cgggatcaag cacaaccgcg
     3541 aactcgtcgg tcaaaacatc ctcctcgcgc tgatccttct gctcatgatc accttccccg
     3601 cagagctctt caacagcacg ctcgaagaga actatgaaga ggtgcagggt tggttcaaaa
     3661 ggctacgacg cgtcgggcga ggactcggtc ggcatttgcg caagctgcct tcgatggtct
     3721 cggttgctct cttcgcgatc gtcgggggcc tcgtctacgg tttcctcgac cccgcattcg
     3781 gcgcggaccg cgactctctc atcaacgtcg ccgggatggc cgctgggatc cttgcgatca
     3841 ccctcacctt cgacctcacg gcggtaaggt cgcttcgacg tcgctaccct gcgagcactc
     3901 ggttacgcat ccttccggga acgatggtcg tcgcggcgct gtgcgttctg ctctcgcgag
     3961 ttgtcgactt ctcgccgggc tacttctacg gggtcatcgc tggctgggtc atcagcgccg
     4021 agacctcgca tagagaggag ggcagagcaa ccgcgatcgg ctcgaccgcg atgatggtca
     4081 tgagcgtggg ggcctggctc ctctggc
//
LOCUS       DATDOZ010000211         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3343649, whole genome shotgun sequence.
ACCESSION   DATDOZ010000211 DATDOZ010000000
VERSION     DATDOZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343649"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VI541_04235"
     CDS             complement(<1..1055)
                     /locus_tag="VI541_04235"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HLF70144.1"
                     /translation="MRTADRIEKLPPYLFATLDKKVSAARAAGVDVVSFGIGDPDLPT
                     PPHIIEAAIAGLRDPVNHQYPSYYGLADFRAAIVRYYERRFGVVLDPDTQVLPLIGSK
                     EGIAHLATAFVDPGDRVLVTDPGYPVYETSTILSGGVPVSVPITAQNGFKPVFSDVGG
                     DLARGAKILWLNYPSNPTSAVCEVGLFEDAVAFCAQHGILLAHDAAYIDVTYDGFKAP
                     SVLQAPGALDLAIEFGSASKTYNMTGWRVGWVVGNASAIEALGRVKTNIDSGIFNAVQ
                     RAAIAALDGPQDALADTLEVYRNRRDILVDALGSAGLIITPPKGSIYLWAPVPEGDTS
                     VSFSEFLLERAGVVVAPG"
     gene            complement(1052..1822)
                     /gene="dapF"
                     /locus_tag="VI541_04240"
     CDS             complement(1052..1822)
                     /gene="dapF"
                     /locus_tag="VI541_04240"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLF70145.1"
                     /translation="MKGKGRVGLEFSKYHGLGNDFLLVFEQEVTPELAVTMCNRNFGV
                     GADGVIEINRSDRADFSFLLFNADGGIAEVSGNGLRCVGKFLYERGLHSDTTIKVEAG
                     GGVKVLELSVQDGVVTGVRADMGAPVDQGEITLHGVVWQRISTGNPHAVTLVDDIDAA
                     PVTTLGPLIERDPSFPDRTNVEFIKVEGDVVHARFWERGVGVTLASGTGSCASLVASG
                     LRRATVRTLGGDLLIEWDPSGRLFMTGPAEHVFDGKLA"
     gene            complement(1819..2745)
                     /gene="miaA"
                     /locus_tag="VI541_04245"
     CDS             complement(1819..2745)
                     /gene="miaA"
                     /locus_tag="VI541_04245"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HLF70146.1"
                     /translation="MRPSSAEPLRVLAIVGPTAVGKSALAMAVAPRLGAEIVSIDSAM
                     VYRGMDIGTAKPSVLDRGQIRHHMIDIADPSESVTVAQFQALARESIADIVSRGSVPL
                     LVGGSGLYFRAVVDPLTFPGTDPTIRSAIQSLGENGGPERLFERLLEVDPAAASRIEP
                     SNVRRVVRALEVFELTGRPFSSYRSDWDSYESIYELEAVGLVAPRDELDERIGARVEA
                     MIAEGLLDEVRALLAKGLRHSMTSVQALGYAQLLAVIEGDISLEQAVGEIKRRTRKFA
                     RRQMGWFRADPRIEWFESDPGGAAIRLLRGVG"
     gene            complement(2726..>2910)
                     /locus_tag="VI541_04250"
     CDS             complement(2726..>2910)
                     /locus_tag="VI541_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HLF70147.1"
                     /translation="GRTFEVLVEGPSKKDPTRLTGRTRSNKVVHFPGDVDGSMFRDVL
                     INSAAPHFLEGEALVS"
BASE COUNT          596 a          926 c          873 g          515 t
ORIGIN      
        1 ccgggagcca ccacgactcc ggcccgctcg agtaagaact cgctgaaact aacagatgtg
       61 tcgccctcag gaaccggagc ccacaggtag atggacccct tgggtggggt gatgatgagc
      121 ccagcggaac caagtgcatc gaccaagatg tcgcgccggt tccggtaaac ctccagggtg
      181 tcggccagtg catcctgcgg gccatcgagt gccgcgatgg ccgcccgctg caccgcattg
      241 aagatccccg agtcgatgtt ggtcttgacg cgaccgagag cctcgatcgc tgaagcgttg
      301 cccacaaccc agcctacgcg ccaacccgtc atgttgtatg tcttcgaggc tgaaccgaac
      361 tcgatagcaa gatcgagagc cccgggggcc tgaaggacgc tgggggcctt gaagccgtcg
      421 taggtcacat cgatatacgc ggcatcatga gcgagcagga tcccatgctg tgcgcagaac
      481 gcgactgcat cttcgaagag gccgacctcg cagaccgccg aggtcgggtt actcggatag
      541 ttgagccaga ggatcttcgc gccccgggca agatcgccgc caacatccga gaagaccggc
      601 ttgaaaccat tctgcgcagt gatgggcacc gacacgggga ccccacccga gaggatcgtg
      661 ctcgtctcat agacggggta gcccgggtct gttacgagga cgcggtcgcc aggatcgacg
      721 aacgccgtgg caagatgggc gatcccctcc ttcgacccga tcaggggcag cacctgggtg
      781 tccgggtcga gcacgacccc gaatcgtcgc tcgtagtacc gcacgatagc ggcgcggaag
      841 tcagcgagac cgtagtagga ggggtactga tggttgaccg gatcgcgcag ccccgctatc
      901 gcagcttcga tgatgtgagg tggggtcggc aggtcgggat ctccgatccc gaacgaaacg
      961 acatcgactc cagcagcacg agcagcagag accttcttgt cgagagtcgc gaagaggtaa
     1021 ggcggtagtt tttcgatgcg gtctgcggtt ctcatgcaag tttcccatcg aagacgtgtt
     1081 cagcaggccc ggtcatgaag agtctcccac ttgggtccca ctcgatcaga aggtcaccgc
     1141 cgagtgttcg cacggtcgcc ctacgcaaac ctgaggcgac aagtgaagcg catgaaccgg
     1201 tacccgatgc gagggtgacc ccaaccccgc gttcccagaa ccgcgcgtga accacatcgc
     1261 cctctacctt gatgaactcg acattcgtgc gatccggaaa ggaagggtct cgctcgatca
     1321 gggggccgag ggtcgtcacg ggggctgcgt cgatgtcgtc gacgagtgtc accgcatgcg
     1381 ggttgccggt cgagatcctc tgccagacaa caccgtgcag ggtgatctcg ccctgatcga
     1441 cgggggcccc catgtcggcg cggactccgg ttacgactcc gtcctggacc gataactcca
     1501 acaccttcac tcctcctcca gcctctacct tgatggtggt atcggagtga aggccgcgtt
     1561 cgtataggaa cttgccgacg catcggagcc cattgcccga cacctcggcg atgccgccat
     1621 cagcgttgaa aagcaggaac gagaagtcgg ctcgatcgga cctattgatc tcgatcacgc
     1681 catcggcgcc cacaccgaag ttgcggttgc acatcgtgac ggcaagttcg ggggtcacct
     1741 cttgctcgaa tacgagcaga aagtcattgc ccaaaccgtg gtacttcgag aactccagac
     1801 ccactctgcc cttgcctttc aaccgacacc tcggagaagc cgaatcgcag caccgcccgg
     1861 atccgattcg aaccactcga tccgcgggtc cgctcggaac cagcccatct ggcgccgtgc
     1921 gaacttcctg gtgcgcctct tgatctctcc caccgcctgt tcgagcgaga tatctccctc
     1981 gatgactgca aggagttgag catagcccag agcctgaacc gaggtcatcg agtggcgaag
     2041 gcccttggcg agcagtgctc tcacctcatc gaggagacct tcggcgatca tcgcctcgac
     2101 cctcgcaccg atacgttcat ccaattcgtc gcgcggcgcc accaacccga ccgcctccaa
     2161 ctcgtagatg ctctcgtacg aatcccagtc cgagcggtaa gacgagaagg gtcggccggt
     2221 caactcgaat acttcgaggg cacgcaccac ccgtctgaca tttgatggtt cgatgcgcga
     2281 agccgctgcg gggtcgacct ccaggagtct ctcgaacagt ctttcggggc ccccgttttc
     2341 gccgaggctc tggatcgcag agcggatggt cgggtctgtc cccgggaatg tcagcggatc
     2401 gaccaccgcc cggaagtaga gcccggaccc tccgactagc agaggcacag agccacgcga
     2461 cacgatgtcg gcgatgctct cgcgagccag ggcctggaac tgcgccacgg tcaccgactc
     2521 agaagggtcg gctatgtcga tcatgtgatg cctgatctga ccccggtcca gcacggaagg
     2581 cttggcagtg ccgatgtcca tgcctcgata gaccatcgca gagtcgatac tcacgatctc
     2641 ggcccccagt cgcggcgcaa cggccatggc gagagcactc ttgccgaccg ccgtcggtcc
     2701 gacgatagcg aggacccgca gcggctcagc tgacgagggc ctcaccctct aggaagtgcg
     2761 gagccgccga gttgatcagc acatcacgaa acatcgagcc atccacgtca cccgggaagt
     2821 gaacgacctt gttagagcga gtgcgtcctg tgagtcgggt tgggtctttc ttcgatggac
     2881 cttcgaccaa aacttcgaag gtcctgccga
//
LOCUS       DATDOZ010000212         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2534681, whole genome shotgun sequence.
ACCESSION   DATDOZ010000212 DATDOZ010000000
VERSION     DATDOZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2534681"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VI541_04255"
     CDS             complement(<1..527)
                     /locus_tag="VI541_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70148.1"
                     /translation="MTDRPVGTSITTRLTASMVSTVLLAVVLVALVGVGMIRQAADRI
                     ALDELHRQAGAIASETSFLRSDPIEAIRFLRNALDLSGAALYRVLDDGSLVILGGAPS
                     VPLTRSDVSILAQGRGIEGVRTSKGSRFVFVAQPVSGNSTLALVLGRPAGLTSRELPL
                     ARRLLAAAVFAMSVAA"
     gene            complement(517..1206)
                     /locus_tag="VI541_04260"
     CDS             complement(517..1206)
                     /locus_tag="VI541_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018832332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF70149.1"
                     /translation="MTTSAPKGEVLIVEDEANIADLVRLYLERDGFKVRHVTTGEEAL
                     ERIDERPPNLVILDIALPGIDGIEVCRRIRSKGDLAVLMLTARDSEVDRVVGLEIGAD
                     DYVTKPFSPRELVARVKAILRRVEGGTSEGAPVSAGGVTVWPQGREVTADDVSVHLTT
                     KEFDLLMYLLQNRGIVVSRPQILGAVWDYEFYGGERTVDAHIRTIRKKLGPALPLTTI
                     RGVGYKIEHDR"
     gene            complement(1274..2734)
                     /gene="trpE"
                     /locus_tag="VI541_04265"
     CDS             complement(1274..2734)
                     /gene="trpE"
                     /locus_tag="VI541_04265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HLF70150.1"
                     /translation="MSHQPAPEVFTELSERFTVIPVWREIASDMETPVSAFRKVTSGS
                     TGFLLESVEGGERWARYSFIGIDPFLVMVSDDGEITWEGEAPSGIEGSDPLKVFRAVL
                     ESYSAPNIPGLPPFHSGAVGYMGYDVVRYLERLPISRPNHLGAPEMVMLFPRTMIAFD
                     HLKQRITVVSNVVGGGTRAEAEKRVDDVVKLLEQPLAYSAFQPHEVDVSLPSSTMTQS
                     EFEAAVLRAREYIREGDIFQVVLSHRFSMELSADPFDVYRVLRLVNPSPYMFFIRHSR
                     ITLFGSSPEPLIKVQGDHVIQRPIAGTRPRGSTGEEDLAIEEEFLRDEKERAEHVMLV
                     DLARNDVGRVAGYGSVSVDELMVVERYSHVMHLVSQVSGTLAPGRDALDALYAAFPAG
                     TVSGAPKIRAMEIINELEPTRRGPYAGVVGYFDLGGNLDSCIALRTAYALDGCLHVQA
                     GAGIVADSDPSKEWWETVNKAKALLTAAAMASSSGP"
     gene            complement(2735..3064)
                     /gene="hisI"
                     /locus_tag="VI541_04270"
     CDS             complement(2735..3064)
                     /gene="hisI"
                     /locus_tag="VI541_04270"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HLF70151.1"
                     /translation="MTLDIKFGPDGLVPAIVQDENTKDVLMLAWMNAQSLRLTLEQGT
                     TWFWSRSRQELWNKGATSGNLQHVVSVAYDCDSDALLVRVHPDGPACHTGETTCFYRS
                     MTEADLS"
     gene            complement(3061..>3808)
                     /locus_tag="VI541_04275"
     CDS             complement(3061..>3808)
                     /locus_tag="VI541_04275"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HLF70152.1"
                     /translation="TAKILSPRKRVLLPDLGAGCSLSATITADQLRQWKSEHPGAIVV
                     SYVNTSAEVKAESDYCCTSANAVQIVESIPADQEILFLPDMFLGAHVERVTGRKMEIW
                     MGECHVHAGIEPVDIEKRLIEYPQADFYIHPECGCSTQALYLVSTGDLPKARTHLLGT
                     TGMGRSAAASDAKQIVVATETGLLHRLRKENPEKEFIAANEAAICRYMKAITPERLLN
                     SLENLDFEIDVPAEIISRARLALERMVQIV"
BASE COUNT          749 a         1176 c         1138 g          745 t
ORIGIN      
        1 gcagctacgg acatggcgaa gaccgcagcg gcgaggagcc ttctggcgag tggaagttcc
       61 ctggaagtca gtcccgctgg gcggcccaac acaagcgcca gcgtgctgtt gccagagacc
      121 ggctgcgcga cgaagacgaa ccgactgcct ttggatgtac ggactccttc gatgcctctg
      181 ccttgagcca ggatcgagac atcggagcga gtgagcggaa cgcttggggc ccccccgagg
      241 atcacgaggc tcccgtcatc gagaacccta tatagggcgg cgcccgagag gtccaatgcg
      301 ttccggagga atctgatggc ctcgatcggg tcgcttcgca ggaacgacgt ttcactcgcg
      361 atcgcgcccg cctgccggtg cagctcgtcg agcgcgatgc ggtcggcggc ctgccggatc
      421 atccctacgc ctaccagagc cacgagaacc acggccaaca gcacggtact caccatcgat
      481 gcagtgagac gagtcgttat cgatgtgccg acaggcctat cggtcatgtt cgatcttgta
      541 gccgacgcct cggatcgttg taagtggcag ggctggcccc agcttcttgc ggatcgttcg
      601 gatgtgggcg tcaaccgtcc gctcgccgcc atagaattcg tagtcccata ccgcaccgag
      661 gatctggggg cgcgagacga ctatccctct gttctgcaag agatacatga gcaggtcgaa
      721 ctccttcgtc gtgagatgaa ccgacacatc gtcagccgtg acttcgcgtc cctggggcca
      781 aaccgttaca ccccccgcac tcaccggggc cccctcactg gttccaccct cgacccgccg
      841 gagtatcgcc tttaccctcg ccaccagctc ccgcggcgag aatggcttgg tgacgtagtc
      901 gtcggcccct atctcgagac ccacgacccg gtcgacctct gagtcccgag cggtgagcat
      961 gaggactgcg aggtcccctt tcgaccggat ccgacggcag acctcgatcc catcgatgcc
     1021 gggcagagcg atgtcgagga tcacgaggtt cggtgggcgc tcgtcgatac gctcgagggc
     1081 ctcttcgcca gtcgtcacat gtcggacctt gaagccatct cgctcgagat agagccgcac
     1141 gaggtcggcg atattcgcct cgtcctcgac gatgagcact tcacctttag gagcggatgt
     1201 tgtcacgacc ttcgatcatc gccgatgtgt tcgatcgctc ctgtcgggca ccttgaaagt
     1261 tcggtgaaga gattcacggg cccgaactcg aggccatggc tgcggctgtg agaagtgcct
     1321 tcgccttgtt caccgtctcc caccactctt tgctcggatc cgagtcggcc acgatccccg
     1381 ctccagcctg aacatgcaaa cagccgtcga gggcgtatgc ggtccgcagc gctatgcaag
     1441 agtcgagatt gccaccgagg tcgaagtagc ccaccacgcc cgcatagggc ccgcgacgag
     1501 ttggttcgag ttcgttgatg atctccatgg ctctgatctt gggggcccct gagacggttc
     1561 ccgcagggaa ggccgcgtag agggcatcga gggcgtcgcg accgggggcc agtgtcccgg
     1621 aaacctgcga gacaagatgc atcacatgcg agtacctctc aaccaccatc agttcatcga
     1681 ccgagacgga gccgtagcca gccacccttc cgacgtcatt gcgagcaaga tcgacgagca
     1741 tcacatgctc ggccctctcc ttttcgtcgc gcaagaactc ctcttcgatg gctaggtcct
     1801 cttcaccagt gctcccccga ggtcgtgtcc cagcgatcgg ccgctggatc acatgatcac
     1861 cctgaacctt gatcaggggt tcgggtgacg aaccgaacag agtgatacgc gagtgcctta
     1921 tgaaaaacat gtaggggctc gggttcacca accgcaggac tcggtagaca tcgaacgggt
     1981 ccgcggacag ttccatcgag aagcggtgcg agagaaccac ctggaagatg tctccctccc
     2041 gtatgtactc cctcgcgcga agaaccgctg cttcgaactc ggactgagtc atcgtcgacg
     2101 agggaaggct tacatcgacc tcatgtggtt gaaaggcaga ataggcaagc ggctgctcga
     2161 gcagtttgac gacatcatcg acacgcttct cggcctccgc cctcgttccg ccccccacga
     2221 cattcgacac caccgtgatc cgctgcttca ggtgatcgaa ggcgatcatg gtgcggggga
     2281 agagcatcac catctcgggg gcccccaggt ggttcggcct cgaaatcggc aaccgctcta
     2341 gataccggac gacgtcgtag cccatatatc ccaccgctcc cgagtggaat gggggcagtc
     2401 cggggatgtt gggggcggag taagactcaa ggacggcgcg gaataccttc agaggatcac
     2461 ttccctcgat gcctgagggg gcctcaccct cccacgtgat ctcgccgtca tcggagacca
     2521 tgacaaggaa ggggtcgatc cctatgaacg agtagcgtgc ccaacgctcc ccgccctcta
     2581 ccgactccag aaggaagccc gtagaaccgg atgtgacctt gcgaaaggcc gatacgggcg
     2641 tctccatgtc tgaggcgatc tcgcgccata cagggatgac agtgaatctt tcagagagtt
     2701 ctgtgaaaac ctcgggagca ggctggtggg acattcagct aaggtcggct tcagtcatcg
     2761 agcggtagaa gcaggtggtc tctccggtgt gacaggcggg cccatcggga tgaaccctga
     2821 ccagcagagc gtcagagtcg cagtcgtacg caactgatac gacatgctga agattccccg
     2881 acgttgcacc cttgttccac aactcctgcc tgctgcgact ccagaaccag gtggtgccct
     2941 gttcgagagt gagtcgtagg gactgcgcgt tcatccacgc aagcatcaag acgtccttgg
     3001 tgttctcgtc ttggacgatc gccgggacga gaccatcggg gccgaacttg atgtcgagcg
     3061 tcaaacgatc tggaccatgc gctctagtgc gagtcgggct ctgctgatta tctcggcggg
     3121 cacatcgatc tcgaaatcga ggttctcgag gctgttgagt aaccgctcgg gtgtgatggc
     3181 tttcatgtac cggcagatcg ccgcctcgtt cgctgcgatg aactccttct cggggttctc
     3241 cttgcgcaga cgatggagca gtcctgtctc tgtcgccact acgatctgct tggcatcact
     3301 cgccgctgca cttcgtccca tgccagtcgt accgagaaga tgtgtcctgg ccttgggaag
     3361 gtcacctgtg gatacgagat agagggcctg tgtcgaacaa ccacactcgg ggtggatata
     3421 gaagtcagcc tgcgggtact cgatgagtcg cttctcgata tcgaccggtt cgatgccggc
     3481 gtgcacatgg cactccccca tccagatctc catcttgcgc ccggtcaccc gctcgacgtg
     3541 ggcccccagg aacatgtctg gcaggaagag gatctcctgg tccgctggga tggactcgac
     3601 gatctggaca gcattcgcgg atgtacagca gtagtcggac tcggctttca cctctgccga
     3661 tgtgttgacg taggagacaa cgatcgcccc agggtgctcc gacttccact gtcgcaactg
     3721 gtcagctgtg atggtcgcgg agagcgagca tccggcaccc agatcgggga ggagcacacg
     3781 ctttcgcggt gagaggatct tggccgtc
//
LOCUS       DATDOZ010000213         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2535254, whole genome shotgun sequence.
ACCESSION   DATDOZ010000213 DATDOZ010000000
VERSION     DATDOZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535254"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VI541_04280"
     CDS             complement(<1..1088)
                     /locus_tag="VI541_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF70153.1"
                     /translation="MPSSREPLFTSSFALVTAASLAYFISLGTLLPIIPRFIEGPLHG
                     NSASVGFGVGSFGLFAFLIRPMAGRLADTRGRKLMMVGGSAMVAVATLGYLAVGHLVA
                     LIPLRLLAGTGEAMFFTGAASAVNDLAPEGRRGEALSLFSLSLYLGLAVGPLIGEGAL
                     GTARFDRVWLVAAIAAIAASLLSLLFADQHSTVNVIKTRLIQTAAIGPGIVVAASVLG
                     FAAMGAFLPLLALDLGLEGSRFLFLIYSAVVIVIRSAGARIPDAFGTRRTVIVSLAIS
                     ALGLAVLSGARSSTWLFAGVPLFAAGQALAFPALMSMAVQRAPSTQRGAVIGTFTAFV
                     DLAFGVGPILLGLVASAAGHGTGGYRAT"
     gene            1177..>3165
                     /locus_tag="VI541_04285"
     CDS             1177..>3165
                     /locus_tag="VI541_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF70154.1"
                     /translation="MERAISDHPDLQSEQAYIDNAYECLGQMQAAARELLDSVLSSTP
                     GGTHQARYQRDVLVLASSERLAQLDLGHESLCFGRMDAASGETFYIGRLAVSGPDMEA
                     LVVDWRVPVAEPFYRATGRHTMGLRRRRHFLADRKTLTAIEDELFSDDPSAGELGLAG
                     PGALLESLERRRTGSMRDIVATIQAEQDEVIRSDLSGVLAVQGGPGTGKTAVALHRAA
                     YLIFTHRQRLERRGMLFVGPNRLFLRYINRVLPALGETGVALTTPSGMYPSIRVVLED
                     PQSAARVKGDLRMAELIRTGIQARQRKLDRTQEMKVGGVLLEVTPRITASAVAGGRRA
                     RGSHNARRSNVRKSLVRSLFGQYVKASGRVPAASLTRVEFTETIEEDGDLERILDQVW
                     PVSDPPSFLHEVLSTPLLDRLGRGLLSSQEIDAIRRDPAHPLTNWTRADVALLDEAAV
                     YLGAIDPTFDEMGEEDIELPTYGHVIVDEVQDLSAMQLRMIGRRCPDGSMTVVGDVDQ
                     VTTPGAPGDWDEILQHLVGRAGARRTALTVNYRTPQEIMDLANRMLGASAAAPSRSVR
                     SSGEEPKLIRSSDVSSDAAREAGRALEIGKVALIADRRLHAGLSAALTALSLDHGVAS
                     ADGIDHEISVCALSEVKGLEFDAVIIVEPVDIWSDAGGR"
BASE COUNT          638 a          944 c         1041 g          542 t
ORIGIN      
        1 aaggtcgccc tgtatccgcc agtgccatgc cctgccgccg aggcgacgag tccaagaagg
       61 atcggcccta ctccaaaggc aaggtcaacg aaggccgtga aggtcccgat aactgccccc
      121 ctctgtgtcg agggcgcccg ctggaccgcc atgctcatga gggctggaaa tgctagcgcc
      181 tgaccggccg cgaacaaggg gacccccgcg aagagccaag tcgaggagcg cgctccactc
      241 agcacggcaa ggcctaacgc ggagatcgcc aatgacacga tgacggtgcg ccttgtcccg
      301 aacgcatcgg ggatgcgagc ccccgcgctt cggatgacaa tcaccacagc ggagtagatg
      361 agaaacagga atctggaccc ctcgagtcca aggtcgaggg caagcagggg gaggaaggct
      421 cccatcgcgg cgaagcccag aacgcttgcc gcgaccacga tcccaggccc gatggcggct
      481 gtctggatca accgtgtttt gatcacgttg acggtgctgt gttgatcggc gaagagcaag
      541 ctgaggagcg aggcggctat ggctgcgatg gccgcgacca accagaccct gtcgaatctg
      601 gcggttccca gggccccctc tcctatgagt gggccaacgg ccagcccgag gtagagagag
      661 aggctgaaca aactgagtgc ctcacctcgt cgcccctcgg gggccagatc gttgaccgcg
      721 cttgcggccc ccgtgaagaa catggcctcg cccgtgccag cgaggagtcg aagtgggatg
      781 agcgcgacca ggtgaccgac ggcgaggtag ccgagggtgg caacagcgac catggccgac
      841 ccaccgacca tcatcagctt gcggcccctg gtatccgcca gccgaccggc catcggcctt
      901 atcagaaacg cgaagagtcc gaacgatccc accccgaacc ccaccgaggc actgttgcca
      961 tgcaggggtc cctcgatgaa cctggggatg atcggcagca gggtgcccag ggatatgaag
     1021 taggcgagtg aagcggctgt gaccagggcg aatgaagagg tgaagagagg ctcgcgcgaa
     1081 ctgggcacgg ctgaaactct agcgcgctgc ccccctctgg cgcgatccga gttgcgacaa
     1141 gggcgcgttt cctgcatcct tcacggttca ccttctttgg agcgcgcgat ctcggatcac
     1201 ccggacctac agtcagaaca ggcctatatc gacaacgcct acgagtgtct tgggcagatg
     1261 caggcggccg cgcgagagtt gctcgactcg gtgctgtctt cgacccccgg ggggacccat
     1321 caagcgaggt atcagcgcga tgtcctggtc cttgcaagtt cagagcgcct cgctcagctg
     1381 gacctgggtc acgagtcact ctgctttggg cggatggacg ctgctagcgg cgaaaccttc
     1441 tacatcggcc gactcgcggt gagtggaccc gacatggaag cgctggtggt ggattggcgc
     1501 gttcctgtcg ccgagccctt ctatcgggcc acaggccgcc acacgatggg tcttcgtcgg
     1561 aggcgtcact tccttgcaga ccgaaagacc ctgacagcga tcgaggacga acttttctcg
     1621 gacgatcctt cggcggggga gcttgggctc gcggggcccg gcgcgctgct cgagtctttg
     1681 gagcgacgtc ggactggctc gatgcgagac atcgtcgcaa cgatccaggc cgaacaggac
     1741 gaagttatcc gctccgacct gtcaggcgtc ctggccgttc aaggggggcc cgggaccggc
     1801 aagacggcgg tggcccttca ccgcgctgct tatctgatct tcacgcatcg ccagcgcttg
     1861 gagcgaagag ggatgctctt cgtaggtccc aatcggttgt tccttcgata catcaaccgc
     1921 gttctgccgg ccctcggaga gaccggcgtc gcactcacaa ccccttcggg catgtacccg
     1981 agcatcaggg tggtcctgga ggatccgcaa tcggccgctc gcgtgaaggg cgacctgagg
     2041 atggccgagc tgatccgcac ggggatccag gcgcgacaga ggaaactcga ccgaacgcag
     2101 gagatgaaag tcgggggcgt tcttctcgaa gtgacgccgc gcatcacggc gtcagcggtc
     2161 gcaggaggaa gaagggcccg cgggtcgcac aacgcgcggc gttcgaatgt tcgcaagtcg
     2221 ttggtgcgca gtctgttcgg acagtacgtg aaggcgtccg gacgcgttcc agcggcgtcc
     2281 ctgactcgag tcgagttcac tgaaacgatc gaggaggacg gagatctgga acggatactg
     2341 gatcaggtat ggccagtgtc ggatccgccg agtttcctgc acgaggtgct gagcacacct
     2401 ctacttgaca gacttggaag aggtctgctg agttcacagg agatcgatgc catccggcgc
     2461 gacccggctc atccgctcac gaactggacc cgcgcagacg tggctctcct agatgaagcc
     2521 gccgtctatc tcggtgcgat cgatccaacc ttcgatgaga tgggcgagga ggacatagag
     2581 ctcccgacct atggccacgt catcgtcgac gaagtgcaag atctctcagc gatgcagctg
     2641 cggatgatcg ggaggcgttg tcctgacggt tccatgacgg tcgtcggtga cgtggatcaa
     2701 gtgaccactc cgggggcccc aggtgattgg gacgagatac tgcagcatct ggtgggtcgg
     2761 gctggagcaa gacgcaccgc cctcacggtc aactatcgaa caccgcagga gatcatggat
     2821 ctcgctaacc gcatgctcgg ggcgtcggca gcggcaccct cgcgatcggt cagaagctcg
     2881 ggcgaggaac caaaacttat ccggtcatcc gatgtctcgt cagatgcggc gcgagaagcc
     2941 gggcgagccc tcgagatcgg aaaggttgcg ttgatcgcag acaggagact gcacgctggc
     3001 ttgagtgcgg ctctgacagc acttagtctc gatcacgggg ttgcatctgc cgatggtata
     3061 gaccacgaga tcagcgtctg tgctctgagc gaagtaaagg gtctcgaatt cgatgcggtg
     3121 ataatcgtag agccggtgga catctggagt gatgccggag gacgt
//
LOCUS       DATDOZ010000214         4969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_182648, whole genome shotgun sequence.
ACCESSION   DATDOZ010000214 DATDOZ010000000
VERSION     DATDOZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182648"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /gene="msrA"
                     /locus_tag="VI541_04290"
     CDS             complement(<1..302)
                     /gene="msrA"
                     /locus_tag="VI541_04290"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HLF70155.1"
                     /translation="MAETKKATFAAGCFWGVEAAFRQVKGVVSTSVGYSGGTTEHPTY
                     KEVCTGRTGHAETVEVVFDPARVSYDDLLNVFWDEHDPTQVNRQGPDVGTQYRSAI"
     gene            complement(295..1377)
                     /locus_tag="VI541_04295"
     CDS             complement(295..1377)
                     /locus_tag="VI541_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLF70156.1"
                     /translation="MAEPENGAGGAGVEELTRKDFTSDQEVRWCPGCGDYAILAAVQS
                     FLPELNVARENMVFVSGIGCSSRFPYYMNTYGMHSIHGRAPTIAGGISMARPDLQVWV
                     VTGDGDGLSIGGNHLIHTLRRNMNLRILLFNNQIYGLTKGQYSPTSERGKVTYSTPLG
                     SLDYPFNPISLALGSEATFVARCVDMDRKYLTEILRAAAEHEGSAFVEIYQNCNVYND
                     GAFDLLTAKGGRANFQIRLVHGQKILFGDDQAKGVVFEDGQAHIVDVAEVGIDRIAVH
                     DAHREDPSLAFALSRLAHGPSGPTPMGIFRQVHRPVYGREMDAQIESAREKRGEGDLA
                     RLLTSGDTWTVPASPSGTNGHGGGHG"
     gene            complement(1364..3214)
                     /locus_tag="VI541_04300"
     CDS             complement(1364..3214)
                     /locus_tag="VI541_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HLF70157.1"
                     /translation="MSSAPVKELDRVVIRFAGDSGDGMQLTGGRFTDATAVMGNDLST
                     LPDFPAEIRAPAGTLAGVSSFQVHFSDHDILTPGDAPNVLVAMNPAGLKANIKELVKG
                     GILIVNSDAFTTGNLEKAGYESDPLDGLNLDGYLVYKVPMSSLTTGALKELGVGKKQA
                     DQSKNMFALGLMIWMFHRPTEPTTAFIQKKFASKPEVAEANLAVFRAGYNFGETAEMF
                     ASTFEVKPAVMKPGHYRNITGNTALAWGLLAASKRSGLPLFLGSYPITPASDILHQLS
                     NFKHFGVKTFQAEDEIAAIGSAIGASFAGQLAVTTTSGPGVDLKSEMIGLAVSLELPL
                     LIVDIQRGGPSTGLPTKTEQSDLLLAMFGRHGEAPVPIVAAHSPSQCFDAAIEAARIA
                     LKYRTPVFLLSDGFLANGSEPWLIPDADALPDISVAFTTEPNHDGEFWPFLRDPETLA
                     RPWAVPGTPGLQHRIGGLEHADGTGNISYDPENHEKMTRLRAAKVAGIAKDIPDATVD
                     DPGGAEVVVVGWGSTWGPIGAAIRRIRSRGKAVAQVHLTHLNPLPRNLGSLLAGYRKV
                     VVPEMNMGQLQKMLRYEYLVDAIGINKVQGLPFKAAELEAKILEIIDGGA"
     gene            complement(3266..3532)
                     /locus_tag="VI541_04305"
     CDS             complement(3266..3532)
                     /locus_tag="VI541_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70158.1"
                     /translation="MALRRARSHRARNAAASSFTVSDLGSKVSILYRIEDDTYPYSEA
                     VGVLSRIESDPEVIYVVIRRDGSQARVPEAAIITSKVVPTRKPG"
     gene            complement(3480..3782)
                     /gene="fdxA"
                     /locus_tag="VI541_04310"
     CDS             complement(3480..3782)
                     /gene="fdxA"
                     /locus_tag="VI541_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HLF70159.1"
                     /translation="MTYIICEPCIGVKDRACVDECPVDCIYEGDDQLYIHPDECVDCD
                     ACLPACPVDAIFPGDNVPAEWKEYIERNAAFFVSNPEASGGAAESPFAPSEERGGE"
     gene            3863..4444
                     /locus_tag="VI541_04315"
     CDS             3863..4444
                     /locus_tag="VI541_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70160.1"
                     /translation="MLPSEILDSLSPQDRRRLIVSAALAALGLLLGIYSLVPPKITVE
                     QTTFQVASLASPIPGRGPASSKTPGYVVANFRLKVGARVKGQRLTFKEIRLFDSRNRM
                     LGVLPIQSITRNLSQTTGPDETKVTESPQVVTGVMFLYPLEPATLTVPAFAQDSFGIA
                     SGTKLRAEIIYKTSMLTHRFKTPYRPANGIGAG"
     gene            complement(4453..4920)
                     /locus_tag="VI541_04320"
     CDS             complement(4453..4920)
                     /locus_tag="VI541_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF70161.1"
                     /translation="MTVSLVEALSALAADVGSESELYEAAVERIVDAAAHYDWVGIYV
                     VEGDRLSLAAWRGPQPTEHVSIPLDQGICGFAATAGETVVVDDVNADPRYLACFLGTR
                     SEIVVPILAGGEVVAEIDIDSDTRGAFGPRDREVLEAAANAIGERVAELRSLD"
BASE COUNT          945 a         1558 c         1500 g          966 t
ORIGIN      
        1 aatatcgccg aacggtactg cgtgccgacg tcgggacctt gacggttcac ctgcgtaggg
       61 tcgtgctcat cccagaagac gttgagaaga tcgtcgtaac tgacgcgtgc ggggtcgaag
      121 accacctcga ccgtctccgc atggccggtc ctgcccgtgc agacctcctt ataggtcgga
      181 tgttctgtcg ttccgcccga gtatccgacc gatgtcgaaa cgactccctt gacttggcgg
      241 aacgccgcct cgactcccca gaagcatccg gcggcgaagg tcgccttctt ggtttcagcc
      301 atgaccgcct ccatgcccgt tggtgcccga gggcgaggcc gggactgtcc atgtgtctcc
      361 cgaggtgagg agccgagcaa ggtctccctc gccccgtttc tctcgagccg attcgatctg
      421 agcgtccatc tcacgcccgt agaccggccg atggacctgt ctgaagatcc ccatcggcgt
      481 tgggccgctc ggaccatggg caagacgcga gagcgcgaat gcgagactgg ggtcctcgcg
      541 atgtgcgtcg tgcaccgcga tccgatcgat gccgacctcg gccacatcga ctatgtgggc
      601 ctgaccgtcc tcgaagacaa ctcccttggc ctggtcgtca ccgaagagga tcttctggcc
      661 atgcaccagc cgtatctgga agttggctcg gccccccttc gcggtcagta ggtcgaacgc
      721 tccgtcgttg tagacgttgc agttctggta gatctcgacg aaggccgacc cttcgtgttc
      781 cgcagcggcg cgaaggatct cggtcaggta cttgcgatcc atatcgacgc atcgggcaac
      841 gaatgttgct tcggagccga gagcgagcga tatggggttg aacgggtagt cgagcgaacc
      901 aagtggggtc gagtatgtga ccttgcctcg ttccgaggtc ggcgagtact gccccttcgt
      961 gagcccgtag atctggttgt tgaatagaag gatgcgcagg ttcatgttcc gccgaagagt
     1021 gtggatgagg tggttcccac cgatcgagag cccgtcgcca tcaccggtga cgacccagac
     1081 ctgaagatct ggcctcgcca tcgagatgcc gcccgcgatg gtaggcgcgc gcccgtggat
     1141 gctatgcatg ccgtatgtgt tcatgtagta cgggaatcga gaagagcagc cgatacccga
     1201 gacgaagacc atgttctcgc gcgcaacatt gagttcaggg aggaagctct gaaccgcggc
     1261 gaggatcgcg tagtcgccgc agccagggca ccagcggacc tcttgatctg aagtgaagtc
     1321 cttgcgtgtc aattcctcga cgccagcacc accggcgccg ttctcaggct ccgccatcga
     1381 tgatctccaa tatcttggct tctaactcgg cagccttgaa cggcaggccc tgaaccttgt
     1441 tgatgccgat cgcatcgacg agatactcgt accgcaacat cttctggagt tggcccatgt
     1501 tcatctcggg cacgacaacc tttcgataac cggcaaggag gctccccaga ttgcgcggca
     1561 gcggattgag gtgagtcaga tggacctgcg caaccgcctt gccccggctc cggattcggc
     1621 gtatcgcggc accgatcggc ccccatgtcg aaccccagcc aacgacaacg acctcggcgc
     1681 cgcccggatc gtcgacggtc gcgtcgggga tgtccttcgc gatgcccgcg accttggccg
     1741 cacgcaggcg ggtcatcttc tcgtggttct ccgggtcgta tgagatgttg cccgttccat
     1801 cggcgtgctc gagcccaccg atgcggtgct ggaggcccgg cgttcccggc accgcccacg
     1861 gtctggcgag agtctctggg tccctcaaga agggccagaa ctcgccgtcg tggttcggct
     1921 ctgtggtgaa cgcaacgctg atatcaggca gagcatcggc atccggtatg agccacggct
     1981 ccgacccgtt cgcgaggaag ccgtccgaga gcaggaagac cggcgtccga tacttgagcg
     2041 cgatgcgggc tgcctcgatc gctgcatcga aacactgcga aggtgagtgg gccgcaacga
     2101 tgggtacggg ggcctcgcca tgacgcccga acatcgccaa cagcaggtcc gactgctcgg
     2161 tcttggtcgg cagaccagtt gaggggccgc ccctttggat gtcgacgatg aggagtggga
     2221 gttcgagcga aaccgccagc ccgatcatct cgctcttgag gtcgacaccc ggcccgctcg
     2281 tggtcgtcac tgccaactga ccggcgaatg aagctccgat cgctgacccg atggccgcga
     2341 tctcgtcctc agcctggaat gtcttaacgc cgaaatgctt gaaattcgag agctgatgaa
     2401 ggatgtccga cgctggtgtg atgggatagg agccgaggaa gaggggcagc cccgaacgct
     2461 tcgaagcagc caacagcccc caggcgagcg ctgtgttgcc ggtgatgttg cgatagtggc
     2521 ccggcttcat caccgcgggc ttcacctcga aggtcgaagc gaacatctcg gcggtctcgc
     2581 cgaagttgta gccagccctg aatacggcga ggttcgcctc ggctacttcc ggcttgctcg
     2641 caaatttctt ctgtatgaac gcagtcgtag gctcggtcgg gcggtgaaac atccagatca
     2701 tgagcccgag agcgaacatg ttcttcgact gatcggcctg cttcttgccg acacccaact
     2761 ccttgagtgc gccggtggtc aagctcgaca tggggacctt gtagacgaga tagccatcga
     2821 ggttcaggcc atcgaggggg tcgctctcgt atcccgcttt ctcaaggttg ccggtcgtga
     2881 acgcatctga gttgacgata aggatgccgc ctttgacgag ttctttgatg ttggccttga
     2941 gccccgcggg gttcatcgcc acgaggacgt tgggggcatc gccgggagtg aggatgtcgt
     3001 ggtccgagaa gtggacctgg aacgaggaga cacctgcgag ggtacccgcg ggggcccgga
     3061 tctcggcggg gaagtcggga agagtggaga ggtcgttgcc catgaccgcg gtggcatctg
     3121 tgaaacgtcc accggtcaac tgcatgccat cgccggagtc cccggcgaac ctgatgacga
     3181 ccctatctag ctccttgact ggagctgacg acatctagct tctccccttc ccttgatcga
     3241 aaacctatct atcctagcat cgcacctatc ccggttttcg cgttggaacc acctttgaag
     3301 tgatgatcgc ggcttcaggg actctcgctt gactcccatc ccggcggata accacgtaga
     3361 tgacctcggg gtcgctctcg atgcgactca gcacgcccac ggcttcgctg tatgggtagg
     3421 tgtcatcctc gatccggtag aggatcgata ccttagaacc cagatcagag accgtgaagc
     3481 tactcgccgc cgcgttcctc gctcggtgcg aacgggctct ccgcagcgcc acccgatgcc
     3541 tcggggttgc taacgaagaa ggcagcattg cgctcgatgt attccttcca ctcggcaggg
     3601 acattgtcgc cagggaagat ggcatcaaca ggacaagccg gtaagcatgc atcgcagtcg
     3661 acgcactcgt cggggtggat gtacaactgg tcatcgccct catagatgca gtccaccgga
     3721 cactcgtcca cacaagcccg gtccttaacg ccgatacagg gctcgcagat gatgtacgtc
     3781 atatcgattt acgctccttg cgatgtcggg tatgtcgaca gttattctaa gcgcgacttt
     3841 acagggtagg cttgcatgcc caatgttacc cagcgagatc ctcgattcct tatcgcccca
     3901 agaccgcagg agattgatcg tctctgccgc cttggccgcg ctcggcctcc tcttggggat
     3961 ctactcgctc gtcccaccga agatcacggt cgaacagacg acatttcagg tggcatccct
     4021 ggctagcccc atcccgggac ggggccccgc atcgtcgaag acgcccggct atgtagtggc
     4081 aaatttccgt ctcaaagtgg gggcccgcgt caagggccag cgcctgacct tcaaggagat
     4141 ccgactcttc gacagccgca accgcatgtt gggtgtcctg cccatacagt cgatcactcg
     4201 caatctctcg caaaccactg gacctgacga gacgaaggtg accgaaagcc cgcaggtggt
     4261 gacgggcgtc atgttcctct atccgctcga gcccgcgacc ctcaccgtcc ccgcattcgc
     4321 ccaggacagc ttcgggatcg cctccggcac gaagctccgg gccgagatca tctacaagac
     4381 gtcgatgctc acccaccgct tcaaaacgcc ctatcgacca gccaacggga tcggcgcagg
     4441 gtaggtcggg ggctagtcca ggctccgcag ctcagcgacg cgttccccga tcgcgttggc
     4501 ggcggcttcg aggacctcac gatcgcgggg gccgaaagcc ccgcgggtgt ccgaatcgat
     4561 gtcgatctct gcaaccacct cgcccccggc gaggatcggc acgacgatct cggatcgagt
     4621 ccccaagaag caggccaggt atctggggtc tgcattcacg tcatcgacca caaccgtctc
     4681 tccggcggtc gcagcgaagc cacagatgcc ctggtccaga gggatactca cgtgttcggt
     4741 gggctggggg cccctccatg cagcgaggct gagccgatcc ccctcgacga catagatacc
     4801 cacccagtcg tagtgggccg cggcatcgac gatccgctcg accgcagctt cgtatagctc
     4861 agactccgag ccgacatccg ctgccaacgc gctcagagct tcgaccaaag aaaccgtcac
     4921 gactcctcct cacgtgcggg gcgaaaggct aagggcactc gctgcagga
//
LOCUS       DATDOZ010000215         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3347715, whole genome shotgun sequence.
ACCESSION   DATDOZ010000215 DATDOZ010000000
VERSION     DATDOZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3347715"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VI541_04325"
     CDS             complement(<1..812)
                     /locus_tag="VI541_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HLF70162.1"
                     /translation="MSERKYGYITFLSDYGYTDEFVGVCHGVIKRIAPQVEIIDITHG
                     IRPQSVFEGAMVLAQSVAYMPHGVHLAIVDPSVGAERLALAIRTVDGSVLVGPDNGLL
                     SMAVRRLGGADACHQISNPEVMLEVVSKTFQGRDIFAPAAAHLANGVAVAQMGAPVDP
                     AGLVGVDIAAPRRHDDHYHATVLHVDRFGNLQLNLGPKEMADAEMKVGDMLEVRLEGH
                     RSLVAFGETFASVGQGELVVTEDSYGLLAVAMNRGSAADLLGASIGSSVIIGP"
     gene            complement(809..1492)
                     /locus_tag="VI541_04330"
     CDS             complement(809..1492)
                     /locus_tag="VI541_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF70163.1"
                     /translation="MRRLAAVLFLLCGLLVSCAPSGSRVSFRSVDGFALGGRIFGSGA
                     RGVILAHMYPTDQTSWFDEAGRLSRQGYQVLTFDFRGYPGSGGTRDIGLIDRDVAGAI
                     RFMTQRRKVRTVVLVGASMGGTASLVAAANEKVDGVAVLSAPVAFRGLDAEPFVAKVE
                     APKLFIASVDDPSGAEGAARQMYKAAFDPDSDLKLLPGREHGTDILKGASGEEAIRVL
                     EDFIKRASS"
     gene            complement(1506..1607)
                     /locus_tag="VI541_04335"
     CDS             complement(1506..1607)
                     /locus_tag="VI541_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HLF70164.1"
                     /translation="MSSVVKKRRKKMRKKKHKKMLKKTRWQRRQQGK"
     gene            complement(1629..>2196)
                     /locus_tag="VI541_04340"
     CDS             complement(1629..>2196)
                     /locus_tag="VI541_04340"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HLF70165.1"
                     /translation="LVNHAREAGCELVIVDTSSLISGTYAEMLKYYKLDLIRPRTVVG
                     FQRGLELDPILGVSTRFFPAEVIALKVESAIVERSAEERLTAREAHLASYFEPPLSRW
                     RVKTTVFMPVLPPETDLAKLDGLVVGLEDGKGTCTGIGFLEYSKEEGVLRMVSSKTEG
                     AQGLRLGSIRIDAEGRTVGRVTIRELFG"
BASE COUNT          463 a          740 c          600 g          393 t
ORIGIN      
        1 gggccgatga tcacagacga accgatcgag gcaccgagga gatcagcagc gctacctctg
       61 ttcatcgcga ccgcaagaag accatacgag tcctcggtga caaccaactc tccctgaccg
      121 accgatgcga acgtctcacc gaaagcgacg agacttcgat gaccctccag cctcacttcg
      181 agcatgtccc cgaccttcat ctcggcatcc gccatctcct tggggccgag gttgagttgc
      241 aggttcccga aacggtccac atgcagaacg gtggcgtggt agtgatcgtc gtgacgacgc
      301 ggcgcggcga tatctacacc cacgagaccc gccgggtcga ccggggcccc catctgcgcg
      361 accgcaaccc cgttcgccag atgcgccgcc gccggagcga aaatatctcg gccctgaaag
      421 gtcttggata cgacctcgag catcacttcc ggattcgata tctgatgaca tgcatcggcc
      481 cccccgagtc ttcgcaccgc catcgagaga agaccgttgt cgggcccgac caaaacagaa
      541 ccatcaaccg tcctgatcgc gagtgcgagc ctctcggccc ccacgctcgg atcgacgatc
      601 gcaagatgaa cgccgtgtgg catgtaggcg accgattgag ccagaaccat cgcaccctcg
      661 aagaccgact gaggtctgat cccgtgtgtg atgtcgatga tctcgacctg gggtgcgatc
      721 ctcttgatca ccccatggca taccccgacg aactcatccg tgtaaccgta gtccgacagg
      781 aatgtgatgt agccatactt gcgctcgctc atgacgacgc ccgcttgatg aaatcctcta
      841 acaccctgat cgcctcctca cccgatgcac ccttcaagat atcggttccg tgttcccggc
      901 cgggtaacag tttgaggtca gaatcagggt cgaaggcggc tttgtacatc tgcctcgcgg
      961 ccccttccgc acccgagggg tcatcgaccg aagcgatgaa gagcttgggg gcttcaacct
     1021 tggcgacgaa cggctccgcg tcgagcccgc gaaacgccac gggggccgag agcaccgcca
     1081 cgccatcgac cttctcgttc gccgcggcga cgagtgaagc cgtgcccccc atgctcgccc
     1141 cgaccagaac gacggtacgc accttgcgcc gctgcgtcat gaaccggatc gcccccgcca
     1201 cgtcgcgatc gatgagcccg atgtcccggg tcccacccga gcccgggtag cctcggaagt
     1261 cgaaggtcag gacctgataa ccctggcgcg acagacggcc tgcctcatcg aaccaggagg
     1321 tttgatcagt cggatacatg tgagccagga tcacaccgcg cgctccagac ccgaagatgc
     1381 ggcccccaag cgcgaaacca tcgacgctgc gaaacgagac gcgcgacccg gatggcgcgc
     1441 aagaaaccaa gagaccgcat aggagaaaca ggaccgctgc gaggcggcgc atcagtgagc
     1501 gggacctact tgccctgctg acgcctctgc catctggtct tcttcaacat cttcttatgc
     1561 ttcttcttac gcatcttttt gcggcgcttc tttacaactg acgacatcta gctctctcct
     1621 ttgttcaact agccgaacag ttcacggatg gtcacccggc caaccgtgcg accttcagca
     1681 tcgatgcgga tggatcccag cctcagaccc tgggccccct cggtcttcga agagaccatc
     1741 cgcagcactc cttcttcctt cgaatactcc aagaaaccga tacccgtgca ggttcccttc
     1801 ccatcctcga gcccaaccac cagaccatca agcttcgcaa gatcggtctc cggtggcaga
     1861 acgggcatga agactgtggt cttgaccctc caacgagaca gcggcggctc gaagtatgac
     1921 gcgagatggg cctcgcgcgc agtgagccgt tcctcagccg aacgctcgac gatggccgac
     1981 tcgaccttga gggctatcac ttctgcaggg aagaagcgag tagagacgcc taggatcggg
     2041 tccagttcga gcccccgctg gaacccaacc actgtgcgcg gccggatgag gtcgagcttg
     2101 tagtacttga gcatctcggc ataggtgccc gagatgagcg aactcgtatc gacgatcacg
     2161 agctcgcatc ccgcctctcg ggcatggttc accagc
//
LOCUS       DATDOZ010000216         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2287081, whole genome shotgun sequence.
ACCESSION   DATDOZ010000216 DATDOZ010000000
VERSION     DATDOZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287081"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VI541_04345"
     CDS             <1..317
                     /locus_tag="VI541_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLF70166.1"
                     /translation="AHPEMIEMTVARRDDEGSTLQQVNRQWGAILSFSSWDRLSEIQV
                     PTLVVHGADDALVPHGNGVNLAARIPGAELVTLEGVGHLVPLEAPGDTLRLIQRFFVK
                     GA"
     gene            318..1163
                     /locus_tag="VI541_04350"
     CDS             318..1163
                     /locus_tag="VI541_04350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF70167.1"
                     /translation="MDLADKVVLITGGARGIGLAMAHAFAAKGARLVLTDIDEEQLAI
                     AENDIKASDSWVKTMKIDVAREADVERMVAQTIDEAGQLDVLISNAGVSASGPAELMP
                     MDDWRWITDINMWAHVYAVRAALPYFKERGSGHLVHVSSAAGILGTPALSAYCMTKFA
                     VFGLAESLAVSLHGSGIGVTLVCPLWVDTDIALHGRVTMDPSLDIDSEKAKELSREMV
                     RSAGIPAEKVADETVAAVEEGRFLVLPHPEVQKFARRKWDDPERYIARAAETLKATQS
                     IFGGS"
     gene            1225..1297
                     /locus_tag="VI541_04355"
     tRNA            1225..1297
                     /locus_tag="VI541_04355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1258..1260,aa:Ile,seq:cat)
     gene            1622..>3915
                     /locus_tag="VI541_04360"
     CDS             1622..>3915
                     /locus_tag="VI541_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HLF70168.1"
                     /translation="MEPDGEFVLPVGTVTLLLADVEGSTRRWEEDRVSMATASSELRD
                     LADELIGRHNGVRPLDQGEGDSFVGAFARPSEAVACALDIQQKASLPLRIGIHVGEVQ
                     RTEEGNYIGPALNRTARVRDSGHGGQVLLSQAAAELVADRLKDASLRDLGTYRLKDLS
                     RPERIFQLLHPDLRSEFPPLRSLDLYRHNLPIHPTRFVGRQSEMHEIEDLLSDSSMVT
                     LSGTGGSGKTRLALQIAAKLVDEFADGVFLCDLARVTDPQGVPAAVATAVGFAVEAGP
                     ALISLLSEKQLFLILDNCEHLISSSAELCQSLLAGAPNVQILATSREPLKVEGETNFV
                     VPSLPVPPEDAIGGIEGLAPYAASELFLDRARRARSDFQLDDQDASAVAAICRRLDGI
                     PLAIELAAARVRVMSPREIAQGLDERFGLLTGGARTAVPRHQTLTASVDWSHDLLTDL
                     ERAVLRRLSVFIGGFTLEAAKEVCSGPKDPDVAPHQAVDLLSLLVDKSLVNVSMTGGQ
                     SRYILLETIRQYAAAKLSEAAEEEDVRRAHRDHFLQSAVGLSELAEGSEGEEAFAAFV
                     RDRENFEAAFRWSCAGGHFEEAARFLSASGDLLLAIWWGEAISWARAVMPSIDKVPEP
                     TRTDVLYRCAPLLANSGPQAHLEPLLKECIEFHRKSGDELRLGRALLTWVQLALTKDE
                     MTGVLEEAVEIAGRLNDLHMTLTAKAHSVFRLGIEDQDLKDRYLDDLVRELNEADAPV
                     VRFWPGASVLEKLLLEDIVRSIAF"
BASE COUNT          744 a         1135 c         1267 g          769 t
ORIGIN      
        1 gcgcacatcc ggagatgatc gagatgacgg tcgcaagaag agacgatgag ggctccactt
       61 tgcaacaggt gaacagacag tggggcgcga tcctgagttt ctcaagttgg gatcgtttga
      121 gcgagatcca agtgccaacg ctagtggttc atggggccga tgacgcactg gtgccccatg
      181 gaaacggagt gaatctcgct gcccgcatcc cgggggccga actcgttacg ctcgaaggtg
      241 tcgggcatct cgttcctctc gaagcaccgg gcgatacgct gcgactcatc caaagattct
      301 ttgtgaaggg agcctgaatg gacctcgccg acaaggttgt gttgataact ggcggagcta
      361 gggggatcgg tctcgcgatg gcgcatgcct tcgcagcgaa aggagcccga ctggttctca
      421 ctgatatcga tgaggagcag ctggcgatcg cggagaacga catcaaggcc agtgactctt
      481 gggtgaagac tatgaagatc gatgtcgctc gcgaggcgga cgtcgaacgg atggtcgcac
      541 agacgataga tgaagccgga cagctcgacg ttctcatcag caacgcgggg gtctcagcct
      601 cggggcccgc cgagttgatg ccgatggatg actggcgctg gatcaccgac atcaacatgt
      661 gggctcatgt ctatgcggtg cgggccgcgt tgccctactt caaggagcgg gggagcggac
      721 acctcgtgca cgtctcgtcc gccgccggga tactggggac cccggccctc tccgcgtact
      781 gcatgacgaa gtttgcagta ttcggacttg ccgagtctct ggcggtctca ctgcatggga
      841 gcggcatcgg agtgaccttg gtctgtccgc tctgggtcga tacggatatc gcactgcatg
      901 gccgagtgac gatggatcct tccctcgaca tcgacagcga gaaggctaag gagctatccc
      961 gcgagatggt gcgcagcgcc gggatccctg ccgagaaggt tgccgacgag accgtagctg
     1021 ccgttgaaga ggggcgcttc ttagtcctgc ctcaccccga ggtccagaag ttcgcgcggc
     1081 gcaagtggga cgaccccgag cggtatatcg ctagagcggc agaaacactg aaggcaacgc
     1141 agtcaatctt cggaggatcg tagggagtaa tcgcgtccga ggtggcaatc ggagacatgc
     1201 cgatatcatg tgccgcgcga gtgggggcct gtagctcagc ggttagagca gtggactcat
     1261 aatccattgg tcgcaggttc gaatcctgcc gggcccacaa actacacatg tgtaattttg
     1321 ccccagcccc cggctagttt gcttttcgag ttatcaacgt tcggtttgac atcgactgtt
     1381 ccgcacgaag ccaccgaaga tccgaaagct gaaaggtgga agcggtcgag atacgtagct
     1441 gccgttgtca aatacgtatg tccttcgatg ttgtttgtcc tcgtctgcct cactgtgagt
     1501 acgtaagagc ggagctaagg ggggtcaagt gggtgctttc gaagatctga cgctgaaggg
     1561 cagccaagaa atgtcggata atctgttacc actgttgcca agggtgaact ggggggagcc
     1621 cgtggagccc gatggggagt ttgttcttcc agtcggcacg gttacgttgc tgctcgctga
     1681 cgtcgagggc tccacgcgcc gctgggaaga agaccgtgtc agcatggcga ctgccagctc
     1741 cgagcttcgc gatctggcgg atgagctgat aggccgccac aacggcgtgc gtccgctcga
     1801 ccagggcgag ggcgacagct tcgttggggc gttcgcccgg ccctcggaag ccgtggcatg
     1861 cgcactggat atccagcaga aggcttccct gccgcttcgg atcggcatcc atgtagggga
     1921 agtccagcgg accgaggaag gcaactacat cgggcccgcg ctgaaccgca ctgctcgggt
     1981 cagagattcc ggccacggcg ggcaggtcct tctgtcacag gcggcggcag agctggttgc
     2041 cgatcgtttg aaagacgctt cgcttcgtga cctcgggact taccggctca aggatctgtc
     2101 acggcccgag cgtatattcc agctcctgca cccggacctt cgcagcgagt tccctccact
     2161 gagatcgctg gacctctacc ggcacaacct tccgatccac ccgaccaggt tcgtcgggcg
     2221 ccagagcgag atgcatgaga tcgaggatct cctttccgat tcatccatgg tgactttgag
     2281 cgggacaggg ggatcgggca agactcggct ggcgctgcag atcgccgcga agctcgtcga
     2341 cgagtttgcc gacggcgtct tcctctgcga tctggcgcgg gtgaccgatc cgcagggggt
     2401 cccggcggcg gtggccaccg cggtcgggtt cgcagtcgaa gcggggcccg cgctgatctc
     2461 gctcctcagc gagaaacagc tttttctgat actcgacaac tgcgaacact tgatctcgag
     2521 ctccgcagaa ctctgccagt cgctcctggc tggcgctccg aatgtgcaga tcttggcaac
     2581 ctctcgcgaa ccgctcaagg tcgagggcga aaccaacttc gtagttcctt cactgcccgt
     2641 tccccctgag gatgcgatcg gaggcatcga gggtcttgcg ccctacgcag cgagcgagct
     2701 gttcctcgac cgcgcccgcc gcgcccgctc ggacttccaa ctcgatgatc aggacgcctc
     2761 ggcggtcgct gcgatctgca ggcgtcttga tgggatcccc ctagcgatcg agctagcggc
     2821 cgccagggtg agggttatgt ccccgaggga gatcgcccag ggtttggacg agcgcttcgg
     2881 actcctcacc ggaggggctc gcacggccgt cccacgccac cagacgctca cggcgtcggt
     2941 ggattggagt cacgaccttc tcaccgatct ggagcgggca gtactgcgac ggctgtcggt
     3001 gttcatcggc ggattcaccc tggaggccgc caaggaggtg tgttcgggac cgaaggaccc
     3061 cgatgtcgca cctcaccaag ccgtagacct gctctcgctg ctcgtcgaca agtcgcttgt
     3121 caacgtgagc atgaccggag ggcagtcccg atacatcctg ctcgagacga tcaggcagta
     3181 cgccgccgcc aagctgtccg aggccgcaga agaggaagac gttcggcgcg ctcataggga
     3241 ccacttcctt cagtccgcgg tcggattgtc cgagctggcg gaggggtccg aaggggagga
     3301 agccttcgct gcgttcgtac gggacaggga gaacttcgag gctgcgttca ggtggagttg
     3361 tgcgggcgga cacttcgaag aggccgccag attcttgagc gcctcgggcg accttctctt
     3421 ggcgatctgg tggggcgaag cgatcagttg ggctagggct gtgatgccga gcatcgacaa
     3481 agtccctgag ccgactcgga ccgatgtctt gtaccgctgt gcgcctttac tcgctaactc
     3541 ggggccgcag gcccacctcg aacccctgct gaaagagtgc atagagtttc accgtaagtc
     3601 cggagacgag ctgcgtctcg gtagggcctt actgacctgg gtgcagctcg cgttgaccaa
     3661 ggatgagatg acaggcgtcc tcgaagaggc ggtcgagatc gccgggcgcc tgaacgatct
     3721 ccacatgacc ctcactgcca aggctcattc ggtctttcga ttggggatcg aagaccagga
     3781 cctcaaggac cgatacctcg acgacctcgt tcgggagctg aacgaggccg acgctcccgt
     3841 tgttcgcttc tggcccggtg cgagtgtcct cgagaagctc ttattggagg atatcgttcg
     3901 ttcgatcgcc ttcag
//
LOCUS       DATDOZ010000217         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_703864, whole genome shotgun sequence.
ACCESSION   DATDOZ010000217 DATDOZ010000000
VERSION     DATDOZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703864"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VI541_04365"
     CDS             complement(<1..398)
                     /locus_tag="VI541_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLF70169.1"
                     /translation="MVRPSATKLLRILLPVLAVVGYLGYREVVTQDSPPDKFQGGIVD
                     PQKYSSPKPSLSSSTQAGTRGKETSTAGTGSLRITVKDQNGRGVGDLPVAITGQIVRT
                     ITTDRLGRFTLNGPAGYYHFEVAPGCLMDR"
     gene            complement(404..784)
                     /locus_tag="VI541_04370"
     CDS             complement(404..784)
                     /locus_tag="VI541_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HLF70170.1"
                     /translation="MKRSIRAPRNCPVCSEVLALTRLSCDNCGTELSGHFPTCEYCEL
                     SDDARETLRIFLSSRGNMKEVERHLGVSYPTARARFEEILVKLGMDSNEAEPESRVLS
                     RDSILEALSRGEIAVEDALNQLAD"
     gene            complement(781..1602)
                     /locus_tag="VI541_04375"
     CDS             complement(781..1602)
                     /locus_tag="VI541_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70171.1"
                     /translation="MTAQADERRKILEMVAEGTLTPSEASTMLDSGPQAPPLPTADDF
                     EEITAVRILGTFRSLRVIADASLRGAHAEGRHRARTDGKTLVIEDDAEFEGNTFIFGV
                     PGGQRGQRRVHGRINGREFRLGGQPPALVVRMNPELPLDIEMTAGSVTVDGIRSQIRA
                     SLTAGSGRFEGVVNPFDIQVEAGTVRVAGRLRDGRSAVRCSAGKVSIDLDRDSDVRIV
                     AKATLGKISLPDGGEGRESQWAGMMIGEKEATIGQGKGLLDIEAVTGAVAVRVVG"
     gene            1752..>3532
                     /locus_tag="VI541_04380"
     CDS             1752..>3532
                     /locus_tag="VI541_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF70172.1"
                     /translation="MLETPSIGPAGQGWLRRTLPLLRHHRWRLRAVVIASVVSMFAIA
                     GTPVVIRQALDQGIVAGHSLRVWLILIVGLALVRFVSGYLVRYQAGRVSLGLEFDLRT
                     LLFEHLQKLDLATHDNIQTGQLTSRANADVRVLQMFLAWMPVLISSLLMTVLAFGVMV
                     TWNPILAIVAISPLPLVILVAMRMRERLFPAAWASQQRAADMADVVDDAVTGVRVVRA
                     FGQEDRELDRFGDRALGYFRSQMRVFRYSARYTPTMALIPSLGFVLVLLIGGSYVHEG
                     RLTIGQFLAFNAYVLQMIAPVQMLGMFIALAQRARASGERILEVLDSAPTIIDAPGAV
                     DLQVSSGKVSFQDVTFGYVPSESVLKGVNFEVAPGERVAIVGGNRSGKSTITTLLPRF
                     YDPNAGVIKIDDQDIKNVTLASLRLQVGIVFEDSFLFSGTIRDNIAYGKPGATEDAVV
                     AAAKAAGAHEFIVMLPGGYDAQVGESGLSVSGGQRQRIALARALLGDPKVLVLDDATS
                     SVDVKTEQEILEALAVLLEGRTTILVASRPSTVSLADRVLVFDQGTLVETGTHAELMQ
                     RSGLYRELMGEASTEGQAVAAGVASSLV"
BASE COUNT          658 a         1067 c         1061 g          746 t
ORIGIN      
        1 tgacgatcca tcagacatcc tggggctacc tcgaagtggt agtagccggc gggtccgttg
       61 agggtgaacc gacccaacct atccgtggtg atcgttcgca cgatctgtcc ggtgatagcg
      121 acggggaggt cgccgacgcc cctcccgttc tgatctttga ccgttatccg aagactgccc
      181 gttccggcgg tcgatgtctc cttcccgcgg gtgcctgcct gggtagatga agagagagac
      241 ggtttgggtg atgagtactt ctgaggatcc acgatcccac cttgaaactt gtcgggagga
      301 ctatcttgcg ttaccacctc gcggtagccg agatacccaa caactgccag aacgggaagg
      361 aggatccgca gcagtttggt tgcggaaggt ctaaccactt cggtcagtcg gcgagttggt
      421 tgagggcatc ctcgacagcg atctcgccgc gagagagtgc ttcgaggatc gagtcacgcg
      481 acaacactcg tgactcgggc tcggcctcat tcgagtccat ccccaacttc acgaggatct
      541 cttcgaatct cgcccgtgcc gttgggtagc tgacccccag atggcgctcc acctctttca
      601 tgtttccgcg agaggagagg aagatccgca acgtctcgcg cgcatcgtct gagagctcgc
      661 agtattcgca ggtggggaag tgccccgaaa gttctgtgcc gcagttgtcg caagagagtc
      721 gcgttagggc gaggacctct gaacatacgg gacagttacg cggagccctg atcgatcgct
      781 tcatcctacg acccgcacgg ccacggcccc cgtgacagct tcgatgtcta gaaggccttt
      841 cccttgtccg atcgtagcct ccttctcgcc gatcatcatg ccagcccatt gtgattctct
      901 gccctcacca ccgtcgggca gcgagatctt gccaagagtg gccttcgcaa cgatccgaac
      961 atcggagtca cgatccaagt cgatgctcac cttgcccgcg gagcatcgga cagccgacct
     1021 accatcgcgc aaccttcctg caacgcgcac ggtacccgcc tccacctgga tgtcgaatgg
     1081 attcacgaca ccttcgaacc tgccactgcc tgctgtgaga cttgctctga tctgggaacg
     1141 gatgccatcg acggtaacac tgccagccgt catctcgatg tcgagaggga gttcggggtt
     1201 catcctcacg acaagggcgg gcggttgtcc gccgagacga aactcacgac cgttgatcct
     1261 tccatggacc cgacgctgac ctcgctgacc accggggacc ccaaagatga aggtgttgcc
     1321 ctcgaactcc gcgtcatctt cgatcacaag cgtcttgcca tcggtccggg cccggtgacg
     1381 accctcggcg tgggctcccc gtagggacgc gtccgcgatc acccgcaacg agcgaaaggt
     1441 ccccaggatg cgcactgctg tgatctcttc gaagtcgtca gcagtaggca gtgggggggc
     1501 ttgggggccg gagtccagca tcgtggaggc ctcgctggga gtaagggtgc cctccgccac
     1561 catctcgagg atcttccgtc gttcgtctgc ttgtgcggtc aaaggggcct cctcactctc
     1621 ggtgatgccg aatctgcttc atggatgata agcagcacct accgataacg caactcgaac
     1681 tgatgaaata gtaagaaaaa cttcaagatt gctaatgcag tgggtatggt ttccctgcta
     1741 tccttctcgg gatgcttgaa actccatcta tcggacctgc cgggcagggc tggcttcgac
     1801 gcaccctccc gcttctgagg caccaccgct ggcgattgcg ggccgtggtg atagcctcgg
     1861 tcgtctcgat gttcgccatc gcggggaccc cggttgtaat cagacaggct ctcgaccaag
     1921 gcatcgtcgc cgggcactca ctacgggttt ggctaatcct gatcgtgggg ctcgcgctgg
     1981 tccgtttcgt ctcgggttac ctcgtgagat accaagcggg ccgagtgagt ttggggctgg
     2041 agttcgacct tcgaacactc ctattcgaac acctgcagaa gttagatctc gcgacccacg
     2101 acaacatcca gaccggtcag ctcaccagtc gcgcgaacgc cgatgtacgc gttctgcaga
     2161 tgttcctcgc atggatgccg gtgttgatca gttccctgct catgacggtc ctcgcgttcg
     2221 gtgtgatggt cacatggaac ccgatcttgg cgatcgtcgc tatatcgccc ctgcctttgg
     2281 tgatcctggt cgcgatgcgc atgcgcgagc gactattccc cgctgcatgg gcgagccagc
     2341 agcgagcggc cgacatggcc gatgtcgtcg acgacgcagt cacaggtgtt cgcgtggtga
     2401 gagccttcgg tcaagaggac cgcgagctcg atcgcttcgg cgatagggct cttggttact
     2461 tccgctcgca gatgcgagtc ttccgttatt cagctcgata cacaccgacg atggcgctca
     2521 tcccgagcct gggtttcgtg ctggttctcc tcatcggcgg gtcgtatgtc catgagggca
     2581 gattgaccat cggtcagttc cttgctttca acgcttacgt gcttcagatg atcgctccgg
     2641 tacagatgct cggcatgttc atcgcccttg cgcagagggc cagggcgagc ggcgaacgga
     2701 tcctcgaggt cctggactcg gcccccacga tcatcgatgc accgggggca gtcgatcttc
     2761 aggtgagttc gggaaaggtc tcgttccaag atgtgacctt cggatatgtg ccctcagaat
     2821 ctgtgctcaa gggcgtgaac ttcgaagtcg caccgggaga acgcgtcgcc atcgtcggtg
     2881 gcaaccgctc cggtaaatcg acgatcacca ccctgctccc gcgcttctac gatcccaacg
     2941 caggcgtgat caagatcgat gatcaggaca tcaagaatgt cacgctcgca tcgcttcgac
     3001 tccaggtggg tatcgtcttc gaggacagct tcctgttctc gggaaccatc cgcgacaaca
     3061 tcgcctacgg caagcctgga gcaaccgaag atgcggtggt cgctgccgcg aaggcggcgg
     3121 gtgcgcacga gttcatcgtg atgctgccgg gcggatacga cgcgcaggtg ggtgagtcgg
     3181 gactctcggt ctccggcggg cagaggcagc ggatagctct ggcgcgtgcg ctcttaggag
     3241 accccaaggt gctcgtcctc gacgacgcga catccagcgt ggatgtgaag actgagcagg
     3301 agatcctcga ggctcttgcg gtgttgctcg aagggcgcac gacgatcctc gtggcgagca
     3361 gaccctcgac cgtctctctc gccgatcgag tactggtatt cgatcaaggc acgttggtcg
     3421 agaccggtac tcatgcggag ttgatgcagc gctccggttt ataccgcgag ttgatgggcg
     3481 aagcatcgac cgagggccag gcggttgcgg cgggagtcgc atccagtctc gt
//
LOCUS       DATDOZ010000218         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1502860, whole genome shotgun sequence.
ACCESSION   DATDOZ010000218 DATDOZ010000000
VERSION     DATDOZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502860"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VI541_04385"
     CDS             <1..930
                     /locus_tag="VI541_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLF70173.1"
                     /translation="GIMEHIEEAGVHSGDSACVLPPYSIGISKMRRIADATQRLAMAL
                     DVIGLINVQFAIKDEDIYCLEVNPRASRTVPFVGKVTGIPLAKVAVRVMLGEKLADMS
                     VLPPRKGEHWWPLDDIKHVGVKEAVLPFARFPGVDTILGPEMKSTGEVMGIDMELGAA
                     FAKSQTGGGWSLPAKGAVFISVADRDKRAIVFPAKRLADLGFQILATEGTADVLARAG
                     VEVKAVRKHSEGGRNIVDQIMDGEVDLVINTPFGRGSRSDGYFIRTAAVMKDIPCITT
                     LAGVQATVQGIESLRRGGIDVKSLQEYLGMALG"
     gene            927..1772
                     /locus_tag="VI541_04390"
     CDS             927..1772
                     /locus_tag="VI541_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HLF70174.1"
                     /translation="MIQQVAEVLSYKKVGDEYHLLTIVAPEIAETARPGQFIELRPPA
                     DRSFILRRPFSIYRVNRRGDWAATVEVLFDIRGGGTAALASLRPHDMVDVIGPVGKGF
                     TIPKKQHSCLLVGGGVGAVPLFFLGEELRAANKRVDILWGAASSSRLVNPIDAKRLGA
                     NTMFATDDGSEGHKGLVTDLLPEMITRGGTEVIYACGPHAMLQAVTRIGIKYSVPVQV
                     AMEELMGCGYGICMTCVQAVWNKEGTDIIHMRTCVDGPVFNGARIAWDANAPKAAAGA
                     DPPGN"
     gene            1772..>2034
                     /locus_tag="VI541_04395"
     CDS             1772..>2034
                     /locus_tag="VI541_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HLF70175.1"
                     /translation="MSRVDMSVDLAGLKLKSPVVTASGCFASGREVAQFADLKKIGAI
                     VVKSMTIEPWPGKPTPRMAEVPSGMLNAIGLQNKGVDHFLAVDL"
BASE COUNT          431 a          560 c          646 g          397 t
ORIGIN      
        1 ggcatcatgg agcacatcga agaggcgggt gttcattcgg gagactccgc ctgcgttctg
       61 ccgccctact cgatagggat cagcaagatg cggaggatcg cggacgcaac tcaaaggttg
      121 gccatggcgc tggatgtcat cggcttgatc aacgtccagt tcgcgatcaa ggacgaggat
      181 atctattgcc ttgaagtgaa cccccgagcc tcgcggaccg tgccattcgt cggcaaggtt
      241 actgggatcc ccctagcgaa agtggcggtg agagtcatgc tcggcgagaa gcttgctgat
      301 atgtctgttc tacccccgcg caagggtgag cactggtggc ctctcgatga catcaagcat
      361 gttggagtga aggaagcggt tcttccgttc gctcgcttcc cgggcgttga caccatcctc
      421 ggaccagaga tgaagtccac cggcgaagtg atgggcatag acatggagct cggggcggca
      481 ttcgccaagt cacagaccgg cggaggctgg agcttgccgg cgaagggagc cgtattcatc
      541 tcggtggcag atcgagacaa gagagcgatc gtctttccgg ccaagagact cgctgacctt
      601 ggcttccaga tcctagcaac cgaagggaca gccgatgtat tggccagggc cggagtcgag
      661 gttaaggcag tacggaagca ttctgagggc ggcaggaaca tcgttgatca gataatggat
      721 ggtgaagtcg acctggtgat caacacacca ttcggccggg gctcccgttc ggatggttac
      781 ttcatccgaa ccgctgcggt gatgaaggac atcccgtgca taacgaccct cgctggggtc
      841 caagcgacgg ttcaaggcat agagtcactt cgtcgaggcg gcatcgatgt caagagtctc
      901 caagagtacc tagggatggc attgggatga tccagcaagt tgcagaggtt ctctcgtaca
      961 agaaggtggg cgacgaatac catctgctga cgatcgtggc ccctgagata gccgagaccg
     1021 cccgcccagg tcagttcatc gagcttaggc ccccggccga ccgaagtttc atcctgcgaa
     1081 ggcccttctc catctaccgt gtcaacagga gaggtgactg ggcagccact gttgaggttc
     1141 tcttcgacat acgaggcggc ggtactgccg cgctggcatc gctgaggccg cacgacatgg
     1201 tcgacgttat cggccccgta ggcaagggat tcacgatccc aaagaaacag cactcgtgtc
     1261 tgctcgtcgg cggcggcgtc ggtgctgttc ctctcttctt cctcggggag gagctaaggg
     1321 cggcaaacaa gcgcgttgac atactctggg gtgcagcgag ctcatcgagg ctggtgaatc
     1381 ctatcgacgc caaacgactt ggggcgaaca ccatgtttgc caccgatgat ggcagcgagg
     1441 gccacaaggg tctagtaacc gacctacttc ccgagatgat cacgcgcggc gggaccgaag
     1501 tcatctatgc ctgcgggcct cacgccatgc ttcaagccgt tacgcggatc gggatcaagt
     1561 attccgtgcc agtgcaggtc gccatggagg agttgatggg ctgcggatac ggcatctgta
     1621 tgacctgcgt tcaagcggtc tggaacaagg aaggcaccga catcatccat atgcggacat
     1681 gcgtcgacgg gccggtgttc aacggagcga ggatcgcatg ggatgcgaat gcacctaagg
     1741 cagcggcggg cgcggacccg ccaggcaact gatgtcgcgc gttgacatgt ccgtggatct
     1801 ggccggactc aagctcaaga gcccagtggt tacagcctca ggctgtttcg cctcgggtag
     1861 agaggtcgcc caattcgctg accttaagaa gatcggagcg atcgtggtga agtccatgac
     1921 gatcgagcct tggcccggca agccaacccc gcgcatggcc gaggttccgt ctgggatgct
     1981 caacgccatc ggtctgcaga acaagggggt ggatcacttc cttgctgtcg acct
//
LOCUS       DATDOZ010000219         3878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3573199, whole genome shotgun sequence.
ACCESSION   DATDOZ010000219 DATDOZ010000000
VERSION     DATDOZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573199"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VI541_04400"
     CDS             complement(<1..540)
                     /locus_tag="VI541_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HLF70176.1"
                     /translation="MQVQRRSRTKDEAAAGVAAGQFGLISRQQAVEAGFDRSAISWRV
                     KSGRWQRVQPGVYAIAGIPESWEQRLLGAQLSLGGRAVVSHRAAALLWGFLATDSAPI
                     ELSVHSSRSPGALDGVIVHRVSSLKGVRKTQGPFELTDPIRTMLDLGVVLGETTLEKS
                     LDKGIRLRCFTGSEIYLAAD"
     gene            698..2545
                     /locus_tag="VI541_04405"
     CDS             698..2545
                     /locus_tag="VI541_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HLF70177.1"
                     /translation="MTRDIAAERAQLEAKYRGKTIPVVLEEVANQFPDIKALNWKQGG
                     EWVSLTYPEMRDEIRKVACGFLAQGLQPGEFVVMLARNIHQHYLADHGAVHARAVGVS
                     LYNTLAPEQIAYIVNHCEATFAVVENRDFLERFLKIKEDVPRLRKVILIEGADKFAGD
                     DWIIGWDEFVASGEAFDKQNPEAFEESYKAVKPDDLLTLIYTSGTTGPPKGVIINHSN
                     ALFVSYSFDALAEREPGDKVVSYLPLAHIAERATSLYIATALAGSIWCCPDLNDLATT
                     LVEVKPQVFFGVPRVFEKIYAGIQAKVAAEPDETRKAFIAQALDVGRQVVMHEQKGVA
                     LPEELAQQHQMIDQMVFSQVRAAIGMDEHRYVVSGAAPISNEVLEFFHSIGIKILEVY
                     GQSEDTGPTSINRPSLNKIGSVGPTLPGVEVKLADDGELLVRGPNVSVGYYKDPDLTA
                     ETFDSDGWLHSGDICEIDEDGYLRVVDRKKEIIITAGGKNIAPSNLEGALKEHVLVGQ
                     AAVIGDRRPFVSALVVLDPEVAPVWAEKNDLSAKTVAELATEPRVVEEIQAWVDKIND
                     RFSNVEKIKKITILPAEWTAESEELTPTLKLKRRVINEKYADEIEGMYN"
     gene            complement(3014..3598)
                     /locus_tag="VI541_04410"
     CDS             complement(3014..3598)
                     /locus_tag="VI541_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLF70178.1"
                     /translation="MPTLWSKTIEAHRREVRDAILHTTVALVAQHGLTSLTMSQIAES
                     TGIGRATLYKYFPGVEEILLAWHEEQINRHLQYLTEVKDGIEDPALRLEAVLEAYALI
                     SHESHGHRDTELGTFLHRDRTLASAQHELQTLVRDLLTHAAEGGAVRSDVSPEELANF
                     CLHALSASGGLRSKAAVHRLVSVTVAGLHPTPGP"
BASE COUNT          804 a         1120 c         1200 g          754 t
ORIGIN      
        1 gtcggccgcc agatagatct cggatccagt gaagcatcgc aggcggatgc ccttgtccag
       61 ggacttctcc aatgtggtct cgccaagtac gacgccaagg tcgagcatgg tccgtatcgg
      121 atcggtgagc tcgaatggac cttgggtctt tcttaccccc ttgaggcttg aaacccggtg
      181 aacgatcacg ccgtccagtg ccccggggct gcgactcgaa tggacggaca gctcgatcgg
      241 agcactgtct gttgctagga aaccccacag cagggcggct gctcggtgcg agacaacggc
      301 ccggcccccg agcgacagct gcgcccctag caatctctgc tcccacgact ctggtatccc
      361 agcgatcgca tagacgccgg gttgtacccg ttgccatcgt ccggacttga ccctccacga
      421 gatggcactc ctgtcgaatc ccgcttcaac ggcttgctgc ctgctgatca gcccgaattg
      481 gccggcggcg accccggcag ccgcttcatc cttcgtgcgg gaacgtcgtt gaacttgcat
      541 gagcccatta aagcggcggg gtgtgacact caatcgactg tatggatagt cgcgtataac
      601 gcgcgtttcc atacagtcga tcctcggggc ggccttgccg cacgggggcc cggtgtcata
      661 cggttatcgc ttcgaactaa cgccagggga ggtaccgatg actcgtgaca tcgctgcgga
      721 acgcgctcag ttggaggcca agtacagggg caagaccatc ccggtcgtgc tcgaagaggt
      781 ggcgaaccag ttcccggata tcaaggccct caactggaag cagggcggcg agtgggtgtc
      841 gctgacctat cccgagatgc gtgatgagat ccgcaaggtc gcatgcggct tcctggccca
      901 ggggctgcag ccaggtgagt tcgtcgtcat gctcgcccgc aacatccatc agcattacct
      961 ggcagaccac ggcgccgttc atgcgagagc cgtcggcgta tctctgtaca acaccctcgc
     1021 gccggagcag atcgcataca tcgtcaatca ctgcgaagcc accttcgctg tggtcgagaa
     1081 ccgtgacttc ctcgagcgtt tcctgaagat caaggaagac gttccgcggc ttcgaaaggt
     1141 catcctcatc gagggggccg acaagttcgc cggtgacgac tggatcatcg ggtgggacga
     1201 gttcgttgca tctggagaag cattcgacaa gcagaacccg gaggcgttcg aagagtcata
     1261 caaggccgtc aagcccgatg acctgctgac gctgatctac acctctggta ccaccggacc
     1321 ccccaagggc gtgatcatca accactccaa cgccttgttc gtctcctact cgttcgatgc
     1381 tctggcggag cgggagcctg gcgacaaggt tgtctcgtat cttccgctgg cccacatcgc
     1441 cgaaagagcg acgagcctct acatcgcgac tgctctcgcc ggttccatat ggtgttgccc
     1501 cgacctgaac gacctcgcca cgacgttggt agaggtgaag ccgcaggtgt tcttcggcgt
     1561 gccgcgtgtc ttcgagaaga tctatgcagg catccaagcc aaggttgcag cagagccgga
     1621 tgagacccgt aaggcgttta tagcgcaggc tctcgacgtt ggccgccagg tcgtgatgca
     1681 tgagcagaag ggtgtggctc tgccggaaga actggcgcag caacatcaga tgatcgacca
     1741 gatggtgttc tcgcaagtac gcgcggccat cggtatggat gagcatcgct atgtcgtgtc
     1801 gggggccgcc cccatctcga acgaggtgct ggagttcttc cattccatag ggatcaagat
     1861 cttggaggtc tacggtcagt cagaggacac aggacctact tccatcaatc gcccatctct
     1921 caacaagatc ggttcggtag ggccaaccct tccgggcgtc gaagtgaaac tagccgacga
     1981 tggcgagctg ctcgttcggg ggccgaacgt atcggtcggt tactacaagg accccgacct
     2041 caccgccgag accttcgact cggacggatg gctgcactcg ggcgatatct gcgagatcga
     2101 cgaggacgga tatctgcgcg tcgtcgatcg caagaaggag atcatcatca ccgcaggcgg
     2161 caagaacatc gcgccgagca atctggaggg ggcattgaag gagcacgtgc tcgtcggtca
     2221 ggcggcggtg atcggggacc gacgcccgtt cgtgagcgcc ctggtcgtac tagaccccga
     2281 ggtcgcaccg gtgtgggccg agaagaacga cctttctgcg aagactgttg ccgagctagc
     2341 gaccgaaccg agggtggtcg aagagatcca ggcctgggtc gacaagatca atgacagatt
     2401 ctcgaacgta gagaagatca agaagatcac gatcctgccc gctgagtgga cggcggagtc
     2461 cgaagagctg acccccaccc tgaaactgaa gcgccgcgtg atcaacgaga agtacgccga
     2521 cgagatcgag gggatgtata actaagtagt tgtctggacc aaggtcactc ggctttggca
     2581 ccgataggga cgcgctaaca cgaccgtccc cctcaaggtc ggcccggacg tcgagtagag
     2641 ctgtgaggtt gccttcggag agttccatgt cgtcgagccg atcaaggatc ttagaggcaa
     2701 cggtagcgaa agccctctca tccagacgcc aggtcacgtc gggtgaggct gaaacaagat
     2761 gctggcggtg tccgtctagc ttggcgacct tccctgagag gacttatctc caagcgtatc
     2821 tctatgcgtg attgaggcgg gagagtgaac atggagactg atccggtttg tgggatggag
     2881 gtcgatccga aggatcctcc gggtggcaag actgagcatg aaggcagagt gacgcgacaa
     2941 cgttcgtcac cctcgccatc gacagcctcg ccagtcaccg ggattgtctt gggggtctag
     3001 agctcgagtg ggattagggt ccaggcgtgg ggtgcaaccc tgcgacagtg accgacacaa
     3061 gccggtggac ggcggccttg gaccgcaggc cgcccgatgc cgagagcgcg tgaaggcaga
     3121 agttcgcaag ttcctcaggc gagacatcag atcgaacggc gcctccctcg gcggcgtgcg
     3181 tcaacaaatc tcgcaccaaa gtttgaagtt catgctgggc actagcaaga gttcggtcgc
     3241 ggtgcaggaa ggtcccaagc tcggtgtctc tgtgtccgtg agattcgtgg gagatgaggg
     3301 cataggcctc caagaccgcc tctagcctta gggctggatc ctcgattcca tccttgacct
     3361 cggtcaagta ttggaggtgt cggtttatct gttcctcgtg ccacgcgagc aggatctcct
     3421 caacgccggg gaagtactta tagagagtcg ccctgccgat tccggtgctc tcggcgatct
     3481 gcgacatcgt cagcgaggtc aacccgtgtt gcgccaccaa cgctacggtg gtgtgcagga
     3541 tcgcgtcgcg cacctcgcgt cggtgtgcct cgatggtctt gctccatagt gtcggcacga
     3601 ttcgattata cgtagcgacg gcatagcgtc gctatgattt gacagagaca ctttgtatta
     3661 taacgctcgg gtgcaaaccg ccagaagtac gaggaggatc cgaacttgac ggacacatcc
     3721 aacgctgaga cgaatcccga agccggtaat gccggaccgc cgggcatcgg atcgaagccc
     3781 ggtatgccac catgggtgaa agtgttcggg atcatcgcga tcggcctgat cgtgatcagt
     3841 gtcatcgggc tccttaccgg ccaacttggc cctggtgg
//
LOCUS       DATDOZ010000220         4314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3052532, whole genome shotgun sequence.
ACCESSION   DATDOZ010000220 DATDOZ010000000
VERSION     DATDOZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3052532"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VI541_04415"
     CDS             <1..821
                     /locus_tag="VI541_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLF70179.1"
                     /translation="SKVPLVLCSGIACDDVHWSFLAPDMGAERMVITWDYPYHGDSGP
                     AADPDNIGLDSLSHHAAALLESLQLDEVVVAAHSMGTQVMFELYRREPSKVRALISIA
                     GSFGHTVGHLYGTQIGTRMLDLLQLGVRLNATAAALAWRIGSTPAFADRFGRALGLIG
                     SGAPVDLMARYFHHVGSMDPDALFRMFREGQNHSAEDLLPNIDVPVLILHGRRDVMTP
                     IQLAEKMNELIPDARLVAFESGAHTLPIEEPVAISQEITAFLAECVEAQQVARD"
     gene            complement(818..1744)
                     /locus_tag="VI541_04420"
     CDS             complement(818..1744)
                     /locus_tag="VI541_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLF70180.1"
                     /translation="MIVDATRAERASLTELADLEPDEVLALMDVDLPDYRDLYYRWER
                     QQWKVQDIDWTVDAEQWADAFDDLEKRSFLWTNAQFYVGENAVTVTLAPYIDAAPREE
                     HRIFLTTQIVDEARHTMFYDRFNEEVIGVEGKSMAARLAEQKIRLNAGYEKLFYELLP
                     AASRALIDDPGNPDRFTEAIVMYHIIIEGTLALTGQKFVLEYLRRRDLLPGFRGGFTA
                     VARDESRHVNFGVRILKEACAADPRQRKVIENYLVEVLPTTFEVLEPPGGDWSYYEPV
                     GYEPEEVIAFSTNSLEKRLRVIGVNSPLANLA"
     gene            complement(1776..2201)
                     /locus_tag="VI541_04425"
     CDS             complement(1776..2201)
                     /locus_tag="VI541_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HLF70181.1"
                     /translation="MKGNLIRVAIPLVIAASIFTGCKKDNPTLSDGGYESQSADSNDE
                     YGYQKTNTSPAAAAPDSVVIQNIAFNPTPLKVKAETTVTWSNKDGFAHTVTSGKRGAP
                     TTLFDFQMGTTATFKFETPGTFDYFCKIHDTMSGTVEVT"
     gene            complement(2270..3385)
                     /locus_tag="VI541_04430"
     CDS             complement(2270..3385)
                     /locus_tag="VI541_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF70182.1"
                     /translation="MRGRSFAALALSVSLTGCGGIPRAELAREEVSIGVLANFTHPDA
                     QTTAMLNGVRMAINEYNHEPTSRYLVNLRRFDTKGSPEGAGTAATRASNLEKSIGVVG
                     PIKTDEIRAAGAVMAAKSVPMIVGNSEEGLLDPSWTNVRRLVASDVQVGRAAGNLVVS
                     RSPLGVASFHDGSESAVNFLSGVHAALAEAKHPLIRTEQVAKIDEGFVKKLISQKPGS
                     VVFAGGRSAAIDFFKTVRRYGSSATIFVGTDLMDRSPEGFERVVVVVPYVPPPDGSVS
                     QPARRFRSAFGADLPSMAVEYYEAGLMLTEAVDEAGTSSGAVMAFLRAQTEFLGDSKR
                     YRYDEHGSLRDAPTWSAHSDGRTWLSDGVIGNAPPKR"
     gene            complement(3382..3936)
                     /locus_tag="VI541_04435"
     CDS             complement(3382..3936)
                     /locus_tag="VI541_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF70183.1"
                     /translation="MVIAEDEAIIRLDLREILIEEGYDVVGEAGDGDKALRLAEELRP
                     DLVILDIFMPGMDGLTAAARIGEKDLCAVLILTAFSQRDLVEQATKTGAMAYLVKPFG
                     KDDLVPAIEMALARWAESRALAAESRDLSERLETRKIVEQAKGVLMSRHGLAEPEAFR
                     TIQKMSMERRLTMREVAQRILDDA"
     gene            complement(3996..>4314)
                     /locus_tag="VI541_04440"
     CDS             complement(3996..>4314)
                     /locus_tag="VI541_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70184.1"
                     /translation="SSHPQVSLSQEDQVKQKVLRRLDQINTEMARYKEELRVATEQLS
                     YQGEVADEAKTRMLVSDTPASRREFKEAEGDLELHRRNHQEMAKKIRELEAQRDRLLE
                     ELI"
BASE COUNT          812 a         1346 c         1237 g          919 t
ORIGIN      
        1 catcaaaggt cccgctggtg ctatgcagcg ggatcgcatg cgatgacgtg cattggagtt
       61 tccttgcacc cgacatgggg gccgaacgga tggtcatcac ctgggactac ccgtatcacg
      121 gcgattcagg ccccgccgca gaccccgaca acatcggcct cgactctctc tcccaccatg
      181 cggctgcgct tctcgagagc ctgcaactcg atgaggtggt cgtcgccgcg cactcgatgg
      241 gcacacaggt gatgttcgag ctctatcgta gggaaccctc caaggttcgc gcattgatct
      301 cgatcgcggg atctttcggc cacaccgttg gccatctata cgggacgcag atcggcaccc
      361 gcatgctcga tcttctgcag ctgggtgtga gattgaacgc cacggccgca gcattggcgt
      421 ggaggatcgg gagcacacca gccttcgccg acaggttcgg ccgagcccta gggctcatag
      481 ggtcgggcgc cccggtcgac ctcatggcaa ggtacttcca tcacgtcgga tcgatggacc
      541 ccgacgccct cttccgcatg ttcagggagg ggcagaacca ctcagctgaa gatcttctgc
      601 ctaacatcga tgtcccggtc ctgatcctgc acggccgccg agacgtgatg actccgatcc
      661 aacttgccga gaagatgaac gaactcatcc cagacgctcg tttagtggcc ttcgagtcgg
      721 gggcacacac cctgcccatc gaggaacctg tcgccatcag tcaggagata accgcattct
      781 tagcagagtg cgttgaagct caacaggttg ctcgcgacta ggccaggttc gcgagcggag
      841 agttgactcc gatgacccgc agtcgtttct ccagcgagtt cgtcgagaaa gcgatgacct
      901 cttcgggttc gtagccaact ggctcgtagt agctccagtc cccaccaggg ggctccagca
      961 cctcgaacgt tgtgggcaga acctctacca gatagttctc gatcactttg cgctgtcgtg
     1021 gatcggccgc gcaagcctct ttgaggatcc gaacaccgaa attgacatgc ctgctctcat
     1081 cgcgcgctac cgccgtgaag ccgccacgga agcccgggag gagatctctg cgccggagat
     1141 actcgagaac gaacttctga ccggtcaagg ccagggtgcc ctcgatgatg atgtggtaca
     1201 tcacgatcgc ttccgtgaat cggtcggggt tccctgggtc atcgatcagg gcgcgagatg
     1261 cggcaggcag caactcatag aagagtttct cgtaccccgc gttcaaccgg atcttctgct
     1321 cggctagccg agcagccatg ctcttgccct ccacccctat gacctcttcg ttgaaacggt
     1381 cgtagaacat cgtgtgtctc gcctcatcga ctatctgagt cgtcagaaag atgcggtgtt
     1441 cttcacgagg ggctgcgtcg atgtacggag ccagagtgac ggtgacggcg ttctctccga
     1501 catagaactg agcattggtc cagaggaagc tgcgtttctc gaggtcatcg aaggcatccg
     1561 cccactgctc ggcatccacc gtccagtcga tgtcctgaac cttccactgc tggcgttccc
     1621 accgatagta aaggtcgcga tagtcgggca ggtccacgtc catcaaggca aggacctcgt
     1681 caggctcaag atccgctagc tctgtgaggc tggccctctc ggcccgagtt gcgtcgacta
     1741 tcaatctgtg ttcctcccgc tcttcgtgat ggagatcaag tgacttcgac agtcccgctc
     1801 atcgtgtcgt ggatcttaca gaagtagtcg aacgtccctg gtgtctcgaa cttgaacgtt
     1861 gcagtcgtgc ccatctggaa gtcgaaaagt gtcgtggggg ctccgcgctt ccccgaagta
     1921 acggtatgtg cgaacccatc cttgttgctc caggtcacgg tggtctcggc cttgaccttc
     1981 aagggtgtcg ggttgaacgc gatgttctgg ataaccacag aatcgggcgc agccgcagcc
     2041 ggcgaggtgt tggtcttctg atacccgtac tcgtcattgg agtcagcgct ttgagactca
     2101 tacccgccat ccgacaaggt cgggttgtcc ttcttgcacc cggtaaagat ggatgcggcg
     2161 atcaccaacg gtatggcgac gcgaatgagg ttccctttca tcattgctcc taacgacctc
     2221 gctcctgcat gggcagtcag cctagccccc ctccacatca tgcacaacat cagcgtttcg
     2281 gaggggcgtt cccgatgaca ccgtccgaaa gccaggtccg tccgtcggaa tgagcagacc
     2341 aggtgggagc atctctcagc gagccgtgct cgtcgtaccg atatctcttg gagtccccta
     2401 ggaactcggt ctgtgccctc aagaaggcca tcacggctcc ggaggacgtt ccggcctcgt
     2461 ccacggcctc tgtcaacatc aaccctgcct cgtaatactc gaccgccatc gagggaagat
     2521 cggccccgaa ggccgagcgg aagcgacgtg caggctggga tacagaaccg tccggtgggg
     2581 ggacgtacgg gacgaccacg acaacgcgtt cgaagccctc cggcgaccga tccatcaggt
     2641 cggttcctac gaatatcgtc gccgacgatc catagcgccg gacggtcttg aagaagtcga
     2701 tagcggccga cctgcctccc gcgaatacga cggatcccgg tttctgcgag atgagtttct
     2761 tgacgaaacc ctcgtcgatc tttgcaacct gttcggtcct gatgagggga tgtttggcct
     2821 cggcgagtgc ggcgtgcacg ccagacaaga agttcaccgc ggactcactg ccatcgtgga
     2881 aagatgcgac gcccaaagga gatctgctca ccaccaggtt ccctgcggca cgtcccacct
     2941 gcacgtcgct cgccaccaat cttcttacgt tcgtccaaga cgggtcgagc agaccctctt
     3001 cggagttgcc gacgatcatg ggcacgctct tggctgccat cactgccccc gcggccctga
     3061 tctcatccgt cttgatgggg cccacaaccc ctatcgactt ctcgaggttc gacgctctcg
     3121 tcgcggcggt tcccgcaccc tccgggctcc ccttcgtatc gaaccggcga aggttgacca
     3181 agtatctaga ggtcggctcg tggttgtact cgttgatcgc catgcgtacc ccattcagca
     3241 tggcggtggt ctgtgcgtcc gggtgagtga agttcgcgag aacccctatc gagacctctt
     3301 cacgagcgag ttcagcgcgg gggatgcctc cacaaccggt aagtgagaca gagagcgcaa
     3361 gtgcggcgaa actgcgaccc ctcatgcgtc gtcgaggatc cgttgagcaa cctcgcgcat
     3421 cgtgagcctg cgttccatgg acatcttctg gatggttcgg aaggcctcgg gctcggctaa
     3481 cccgtgcctg ctcatgagaa ctcctttggc ctgttctacg atcttgcgag tctcaagtcg
     3541 ctcagagagg tcccggctct ccgcggcgag cgcccggctt tcggcccacc gtgcgagggc
     3601 catctcgata gcgggaacga ggtcgtcctt cccgaaaggt tttacgaggt acgccatggc
     3661 cccggtcttg gtcgcctgct ccacgagatc gcgttggctg aaagcggtga ggatcagaac
     3721 agcgcagaga tccttctccc cgatacgggc tgccgccgtt agtccgtcca taccgggcat
     3781 gaagatgtcg aggatgacca ggtcgggcct cagttcttcg gcgagacgca gagccttgtc
     3841 gccatcgccc gcctcgccga cgacgtcgta cccctcttcg atgaggatct cgcgaaggtc
     3901 gaggcggatg atggcctcgt cctcggcgat gactatgcgc gctgagtgtt tctccatggc
     3961 gagcaaccat aacgcagccg ggctacgaca gtccttcaga tcagttcttc taggagacga
     4021 tcacgctgcg cctcaagttc acggatcttc ttcgccatct cttggtgatt gcgtctgtga
     4081 agttctagat cgccctcggc ctctttgaat tcgcggcggg atgcgggcgt gtcgctcacg
     4141 agcatcctcg tcttcgcctc atcggctacc tcaccctggt aggagagctg ctcggtcgcc
     4201 actctcagct cttccttgta gcgcgccatc tcggtattga tctggtccaa tcggcgcaat
     4261 accttttgct tcacctgatc ctcctgagac aacgatacct gcgggtgcga tgat
//
LOCUS       DATDOZ010000221         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2288612, whole genome shotgun sequence.
ACCESSION   DATDOZ010000221 DATDOZ010000000
VERSION     DATDOZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288612"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VI541_04445"
     CDS             <1..223
                     /locus_tag="VI541_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Required for the synthesis of the thiazole moiety;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HLF70185.1"
                     /translation="PLVGSDKLEIEAVGRKIGTYEISIEPHQDCCVLFVPRRVTTAAR
                     LPALEAAESHLDLDVLIEKAIANAEVIEI"
     gene            326..757
                     /locus_tag="VI541_04450"
     CDS             326..757
                     /locus_tag="VI541_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HLF70186.1"
                     /translation="MAAATQREEDSAPARQGVRLLRRSTSNRMIAGVCGGLGRYLGID
                     ATLIRIAWVLLVLGAGTGILLYLIAWLVIPPQRAWEDLGPPARPIRTDPAMILGAALV
                     IIGGLVMLDRHLPPINALLWPLGLIGIGAVILLGGARRRNN"
     gene            complement(754..1110)
                     /locus_tag="VI541_04455"
     CDS             complement(754..1110)
                     /locus_tag="VI541_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HLF70187.1"
                     /translation="MSPSGPYLEEAARLLAEGDYFMAHETLEEYWIDSPTEERDLYQG
                     LIHMAVGLLHHVRGNRRGAELQFGKARARLASYPDEFGGVDVKSVRTFLDLVATSLAP
                     GGALTPPIFLIPERST"
     gene            complement(1113..1583)
                     /locus_tag="VI541_04460"
     CDS             complement(1113..1583)
                     /locus_tag="VI541_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70188.1"
                     /translation="MEVSRKVLGGLSALGLAGLVAAGVIAVGTPDHARRVRADERRSE
                     TLQSLDFLLAEHARAHEAQGLPASLDDVDGFANYPQDPREDPITHRRFTYERVSSSRY
                     RICADFDLALDGTDTSSTRYVEPGVGGQFFRHGAGEHCFVVKAQDFPVPGKYPD"
     gene            complement(1574..2140)
                     /locus_tag="VI541_04465"
     CDS             complement(1574..2140)
                     /locus_tag="VI541_04465"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HLF70189.1"
                     /translation="MIFAALGTLAVLSVIASVVSVVVIIVRWAVLRRKHEVGLSGPRD
                     AFLYLLSTACLYATVIGAMILVFGLADIWFPEEGRTSGFSGPVRAGIAIAIVTAPLFF
                     ILNSIIASRLRKGDMDPHSTLRRGLTYLTLLLIVATALGDLMFVINALLGGEMTAVTV
                     VRTAGVLVIAAAVYLYHSSIVREDALWK"
     gene            complement(2137..>2442)
                     /locus_tag="VI541_04470"
     CDS             complement(2137..>2442)
                     /locus_tag="VI541_04470"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HLF70190.1"
                     /translation="ASVSVVIVSFPTFLFVTSVVRRKLASGDMSARALMRRVLTYGML
                     FLLTVSLVIDLIVVIFLLLGGELTSSALIKGGVTLAVAGLAFLYYLAELREMKGAEA"
BASE COUNT          518 a          772 c          737 g          415 t
ORIGIN      
        1 accgctcgtt ggaagcgaca agctcgagat cgaggcagtc ggtcgcaaga tcgggacgta
       61 cgagatctcc atcgagccgc accaggactg ctgcgtcctc ttcgtccccc gccgagtcac
      121 gaccgccgcc cggctaccgg ccctcgaagc cgccgagtct cacctcgacc tggatgtgtt
      181 gatcgagaag gccatcgcca acgccgaggt catcgagatc tgatctcggt gaaaaatcat
      241 ccccgagtag gaggccgaat cggccccggg gtagatgatt caccaccgcc accgcactat
      301 cctttcgatc atggattggg aagagatggc ggccgcaacg cagagggaag aagactcggc
      361 ccccgcgcgg cagggtgttc gactccttcg aaggagcact tcgaatcgga tgatcgcggg
      421 ggtatgcggg ggcctcgggc ggtatctcgg catcgatgcg accctgatcc gcatcgcatg
      481 ggtgctcctc gtgctcgggg caggaacggg gatccttctt tatctgatcg cgtggctggt
      541 catcccgcca cagcgcgcgt gggaagacct cggccccccg gcgcgcccga tccgcaccga
      601 ccccgcgatg atcttgggag cggctctcgt catcatcggg ggccttgtga tgctcgaccg
      661 tcaccttccg ccgatcaacg cactgctctg gccactcgga ctcatcggca tcggcgccgt
      721 gatcctgctg ggtggggcga gacgccgaaa caactaggtt gaacgctcgg ggatgaggaa
      781 tatcggcggg gtcaacgcgc cacccggcgc cagtgatgtc gcaaccaggt cgaggaatgt
      841 ccttacgctc ttcacgtcga caccaccgaa ctcatcggga taggaagcaa ggcgcgctct
      901 ggcctttccg aactgcaact cggcccccct gcgattgccg cggacgtggt ggagcaaccc
      961 cacggccata tgtatcagtc cctggtagag atctcgttcc tcagtgggcg aatcgatcca
     1021 gtactcctcg agcgtctcgt gggccatgaa gtagtctccc tcggccaaca gacgcgctgc
     1081 ctcttcgaga tagggcccgc tgggagacaa agctagtccg ggtattttcc gggaaccggg
     1141 aagtcctgcg ccttcaccac gaagcagtgc tccccggccc cgtgtcggaa gaactgccca
     1201 ccgacaccgg gctcgacgta gcgcgtcgaa gatgtgtcgg tcccatccaa agcgaggtcg
     1261 aagtcagcac agatccgata tcggctcgag ctcactcgct catacgtgaa tcgtcgatgc
     1321 gtgatggggt cctcgcgcgg atcttggggg taattcgcga agccgtcaac atcatcaagt
     1381 gatgcgggca ggccctgggc ctcgtgggcg cgcgcatgct cggcgagaag gaagtcgagg
     1441 gactgcaagg tctccgatcg acgctcatcg gccctgaccc gtctagcatg atcaggagtt
     1501 ccaaccgcta tcactcctgc agcaaccaac ccggccaaac cgagggctga gaggcccccg
     1561 aggaccttgc ggctcacttc catagggcgt cctctctgac gatagacgaa tggtagaggt
     1621 agaccgctgc ggcgataaca agaacgcccg cggtcctcac gacagtgacc gcggtcatct
     1681 cgccgcccag tagcgcgttg atgacgaaca tcagatcacc cagcgccgtt gcaacgatca
     1741 agaggagggt tagatatgtg agtcctctgc gcagggtcga gtgcgggtcc atgtcaccct
     1801 tgcgcaggcg ggacgcgatg atcgagttca ggatgaagaa gagcggcgca gtgacgatcg
     1861 ctatagcgat gccagccctc actggccctg agaacccgga ggtcctcccc tcctcaggga
     1921 accagatatc ggccaatcca aacacgagga tcatcgcacc gatgaccgtg gcgtatagac
     1981 aggccgtcga gagcagatag aggaacgcgt cccgggggcc cgatagtcct acctcatgct
     2041 ttcgccggag cacggcccac cgcacgataa tcaccacgac actcacgacc gacgcgatca
     2101 ccgataggac cgcaagcgtt ccgagcgctg cgaagatcaa gcctcggccc ccttcatctc
     2161 tcgaagttca gcgaggtagt agaggaaggc aagaccggcg accgcaagtg tcactccacc
     2221 cttgatcaga gcactcgatg tcagctcgcc tccaaggagg agaaagatca cgacgatgag
     2281 gtcgatcaca agactcacgg tgagaaggaa gagcatcccg tatgtgagca ccctgcgcat
     2341 gagtgcccgc gccgacatat ctccgctagc caacttcctg cgcacgacgc tcgtcacgaa
     2401 gaggaacgtt gggaatgaga cgatcacaac cgagacggaa gc
//
LOCUS       DATDOZ010000222         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1505316, whole genome shotgun sequence.
ACCESSION   DATDOZ010000222 DATDOZ010000000
VERSION     DATDOZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505316"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VI541_04475"
     CDS             <1..376
                     /locus_tag="VI541_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010057830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLF70191.1"
                     /translation="EGVSVGFVGGGLPTPLHIAGEIPEEEYNAKLDGLGEVDIVCSHM
                     PPDIPELTYDLVARRRERGSTHLLEYIRAVQPARVFFGHIHQPMVSSMFVGRTHLINA
                     GYFRKTKRALPLLWGGVQAHPG"
     gene            438..863
                     /locus_tag="VI541_04480"
     CDS             438..863
                     /locus_tag="VI541_04480"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLF70192.1"
                     /translation="MADEGQESIHIEATPEKVLDVVADVEAYPSWMSAFKKAAVEERD
                     DAGRPLRASFEVDAMIKTVEYTLEYTYADGSITWRSVDGNVKQIDGGYTVVSDGDGSL
                     VTYDYAIDPGFSVPGFLRRQGVKMMVSTALNDLKKRAEA"
     gene            870..1118
                     /locus_tag="VI541_04485"
     CDS             870..1118
                     /locus_tag="VI541_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70193.1"
                     /translation="MDPAEESSHPQPLRLAHQAHASGECPYPCPVCMGIDLVTQLHPE
                     VAAHLSAAAREFMMAAMAFVSSLAEKDAAARAEKIPFD"
     gene            1176..1625
                     /locus_tag="VI541_04490"
     CDS             1176..1625
                     /locus_tag="VI541_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70194.1"
                     /translation="MKGGVVGRLLMKITLAATVAVAALSAGMANATHGGDDFTATGSI
                     RSSSPFGRFTPCANATTLAATQGVTQFIVDIPASTWGHDFVLTSDSGTAGFEAYLYAR
                     SGATCTKLNEVGSDSGTVVAGTTHAVVVLSSPVASLGEGFTLTVDSL"
     gene            complement(1694..1996)
                     /locus_tag="VI541_04495"
     CDS             complement(1694..1996)
                     /locus_tag="VI541_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HLF70195.1"
                     /translation="MVHELRRRDRHCRYPSCRSRKGLDVHHKVHWAHGGKNDPDNLVL
                     LCEAHHIYVHEAGYKIRGSPPKVTIERPGREPLGVGPPRLDEATSDLFEYEVQGVL"
BASE COUNT          439 a          676 c          681 g          445 t
ORIGIN      
        1 agagggggtt agtgttggct tcgttggcgg cggtcttccg acgccccttc atatcgccgg
       61 cgagatccca gaggaggagt acaacgctaa gctcgatggg ctcggtgagg tcgacatcgt
      121 ctgttcacac atgccccccg atatccccga gttgacctac gacctggtcg cccgccgtag
      181 ggagcgaggt tctacccacc tgctcgagta catccgagcc gtgcagccgg cgcgcgtgtt
      241 cttcggtcac atccatcagc ccatggtctc ctcgatgttc gtgggccgga cccatctgat
      301 caacgccggt tatttccgca agactaagcg tgcacttccg cttctatggg gtggcgtcca
      361 ggctcatccc ggctagtatc gggcggctac acaatagaat cagcggaccc gacggatcca
      421 gataaggagt gttggcaatg gctgacgagg gccaggagtc gatccacatc gaggccactc
      481 ccgaaaaggt cctcgacgtt gtagcggacg tagaggcgta tccgtcatgg atgtcggctt
      541 tcaagaaagc agccgtcgag gaacgggatg atgcagggcg gccactacgc gctagcttcg
      601 aggtcgacgc catgatcaag accgttgaat acacgcttga gtacacatac gccgacggtt
      661 ccatcacttg gcggagcgtc gatggcaacg tcaagcagat agacggcggt tacaccgttg
      721 tctcagatgg cgatggctca ctcgtcacgt acgactacgc gatcgaccca gggttctcgg
      781 tgcccggatt cctgcgcaga cagggcgtca agatgatggt ttcgactgcc ctcaacgacc
      841 tcaagaagcg agcggaggct tagcgctcga tggaccctgc cgaggagtcc tcccaccccc
      901 agcctttgag gcttgcgcac caagctcacg catcgggaga gtgtccttat ccatgtccgg
      961 tgtgtatggg tatcgatctg gtcacccagc tgcatcccga ggtcgctgct catctgagcg
     1021 cggccgctcg cgagttcatg atggccgcca tggcattcgt cagctcgctt gccgagaagg
     1081 atgctgcggc gagggccgag aagatcccgt tcgactgaaa gacctagccc agggaaggaa
     1141 tcctctcaag tccgccgaac agtattgaga gtcaagtgaa aggcggagtt gtgggcaggc
     1201 ttctcatgaa gatcaccttg gcggcgacgg tagcagtcgc cgcactttct gccgggatgg
     1261 ccaatgccac acacggcggc gatgacttca ctgcaacggg atcgatccgt agcagttctc
     1321 cttttgggag attcacccct tgtgccaacg ccacgacatt ggctgcgacg cagggcgtca
     1381 ctcagttcat cgtggatatt ccggcaagta cgtggggtca cgactttgtg ctgacaagcg
     1441 acagcgggac cgctggattc gaagcgtacc tctatgcaag atccggtgcg acctgcacga
     1501 agctcaatga agtgggtagc gactcgggga ccgttgtcgc aggaaccaca cacgcggtcg
     1561 tggtgctctc ctctccggtg gcgagcctag gtgagggctt tacactgaca gtcgactcgt
     1621 tgtgatgaac gaaagcagct gcgtggcaag ggatcccgcg cattcactct ccggcacctg
     1681 cccgactccc acttcacagg accccctgca cttcgtactc gaataggtcg ctcgtagcct
     1741 cgtcaaggcg cgggggaccg accccaaggg gctcccgacc gggcctctcg atggtcacct
     1801 tcggaggtga cccccgtatc ttgtatccgg cctcgtgaac atagatgtgg tgagcctcac
     1861 agagaagtac cagattatcg gggtcattct tcccgccatg cgcccagtga actttgtggt
     1921 ggacatcgag ccccttgcgg ctccgacacg aggggtatcg gcagtgtcga tcacgccttc
     1981 gcagttcgtg gacgatccgc cgtgggaccg tccgccggcc cttgtgatgc gctggggctt
     2041 acccgaggcg tcatctaggc agaagtgcac cgatgcgtcg caggctagcc gagcggcggt
     2101 ctcgatactg atagatggac catcctcgat ctcggcatgc ccttcgcccc caccgagagc
     2161 ccctgcatcg acatggacga cgatctgggt cctctcacga gcagaagatg ggtcggccag
     2221 agcggccagg gcatcggccc t
//
LOCUS       DATDOZ010000223         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_436304, whole genome shotgun sequence.
ACCESSION   DATDOZ010000223 DATDOZ010000000
VERSION     DATDOZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436304"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VI541_04500"
     CDS             <1..1611
                     /locus_tag="VI541_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HLF70196.1"
                     /translation="KLKELVDSKKVAGISGGRNESNRKGTRIVIELKRDAIPQVVLNN
                     LYKYTQLQDSFGMNMLALVDGVQPRTLPLREFLSHYIAHQIEVITRRTAFRLRKAEER
                     MHVLEGLLIALDHLDAVIKLIREADSADDARTKLMGRYELSEIQANAILDMQLRRLAA
                     LERRKIKDEAAELTKTIGELKAILASPAKVRKIIKDELIAIKEKFANPRRTQIIADEG
                     SFDIEDLIPDEDVVVTITRSGYIKRVKSSIYKTQGRGGKGVIGAKPKEGDLVQHMLTT
                     TNHAYVLVFSNRGKVYRIKAHEIPEKDRTARGTSIRNILPFVAEEAVAEVVDTRDFET
                     HRYLVIATRTGIIKKTEFNAYNSSRRDGIIAINLRDNDEVVRVRPTAGDDELIMVSRK
                     GMGIVFAETDVRPMGRTAGGVIGMRLGAEDDVVSFDVVDQNAELLLVTDAGYGKRTLL
                     DRYPRQRRGGKGVKTAKLTSKRGSVQGAMVVRVGHEIFLIASDGQVIRIAVKDISRQG
                     RDTTGVRVMTLDPGVILTAVAHVTEDDE"
     gene            1618..2064
                     /locus_tag="VI541_04505"
     CDS             1618..2064
                     /locus_tag="VI541_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HLF70197.1"
                     /translation="MGTYRSRKGNPGEFRAVGASLTDLFETCAQATFDLLYDTSLVGF
                     EREVSVVASGPDLEHLLAEWIGETVSLFEEKEFVPGDFIVVEVGLPTNRRYGDNDLIV
                     RSAVRGRFLGEWFVDPGRRAMPIDAKDVRITSNRRKHTADVRVTLR"
     gene            2141..>2532
                     /locus_tag="VI541_04510"
     CDS             2141..>2532
                     /locus_tag="VI541_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70198.1"
                     /translation="MQDETSEKDEKKQPKGEEVADSNPVPRDDALDSFWSVTRPDPGP
                     LLPLFEPPEPIPEPEIEPVFSWTMESESGGGAAFAEPEPLVEERSDTAVPEPAAGQEA
                     EAPRSLWWEQPEPSLEDILLEPQSTSAEV"
BASE COUNT          542 a          796 c          779 g          415 t
ORIGIN      
        1 aagctcaaag agctcgtcga ctccaagaag gtcgccggga tctcgggagg ccgcaacgag
       61 agcaaccgca agggcactcg catcgtcatc gaactcaagc gcgacgcgat cccgcaggtg
      121 gtcctgaaca acctctacaa gtacacgcag ctgcaggact ccttcggcat gaacatgctc
      181 gccctcgtcg atggcgtgca gccgcggacc ttgccgctgc gtgagttcct cagccactac
      241 atcgctcacc agatcgaggt catcaccagg cgcacggcct tccggctgcg gaaggccgag
      301 gagcggatgc atgtcctcga agggctcctc atcgcactcg atcatctcga cgcggtcatc
      361 aagctgatcc gcgaggcaga ttcagccgac gacgcccgca ccaagttgat gggccggtat
      421 gagctgagcg agatacaggc caacgcgatc ctcgacatgc agttgcgaag actcgccgct
      481 ctcgagagac gcaagatcaa ggacgaagct gccgaactta cgaagacgat cggggaactg
      541 aaggccatcc tcgcctcgcc ggccaaggtc cgcaagatca tcaaggacga actgatcgcc
      601 atcaaggaga agttcgccaa cccgcgacgc acgcagatca tcgccgatga aggttcgttc
      661 gacatcgagg atctgatccc cgacgaagac gtcgtcgtga ccatcactcg ctccggctac
      721 atcaaacgcg tgaagtcgtc catctacaag actcagggcc gtgggggcaa gggcgttatc
      781 ggtgccaagc ccaaagaggg cgacctcgtg cagcacatgc tcaccaccac caaccatgcg
      841 tatgtgctcg tcttctcgaa ccggggcaag gtgtaccgca tcaaggccca cgagatcccc
      901 gagaaggatc gcaccgcccg aggcacctcc atcagaaaca tcctgccctt cgtagccgaa
      961 gaggctgttg ccgaggtcgt cgacacgcgc gacttcgaaa cccatcgtta tctggtgatc
     1021 gccacacgca cggggataat caagaagacc gagttcaacg cttacaactc gagcagacgc
     1081 gacgggatca tcgccatcaa ccttcgcgac aacgacgagg tcgtgcgggt gaggcccact
     1141 gctggcgacg acgagctgat catggtctcg cgcaagggga tggggatcgt cttcgccgag
     1201 acggatgtcc gcccgatggg gcgcaccgct ggcggggtca tcggcatgcg actgggggcc
     1261 gaagatgatg tcgtctcttt cgatgtggtc gaccagaacg ccgaactact tctcgtcacc
     1321 gacgccggtt acggcaagag gaccctgctc gatagatacc cgcgacagcg gcgcggtggc
     1381 aagggcgtca agactgcgaa gctcacgagc aagcgaggct cggttcaggg tgcgatggtt
     1441 gtgcgcgtcg gccacgagat cttcctcatc gcgagcgacg gtcaggtgat ccgcatcgcc
     1501 gtcaaggaca tatcacgaca ggggcgcgat acgaccgggg tccgtgtcat gacgctcgac
     1561 ccaggagtga tactcactgc tgtcgctcat gtaaccgagg atgacgagta gggccgattg
     1621 ggcacctatc gctctcgtaa ggggaacccc ggagagttca gggctgtcgg cgcgtccctg
     1681 accgacctat tcgaaacctg tgcacaggcc accttcgacc tgctctacga cacctctttg
     1741 gtggggttcg agcgcgaggt ctcagtcgtg gcctccggac cggacctcga acatcttctc
     1801 gccgagtgga tcggcgagac cgtgtctctc ttcgaggaga aggagttcgt accaggcgac
     1861 ttcatcgtgg tcgaggtcgg gctccccacc aaccgacgct atggcgataa cgatctcatc
     1921 gtccgcagtg cggtgcgggg aaggttcctc ggtgagtggt tcgtggaccc cggcagacgt
     1981 gcgatgccga tcgacgccaa ggatgttcgc atcacctcca accggcgcaa acacaccgcg
     2041 gatgtgcggg tgaccttgcg ctgacgcagg ggccccctgg agtatcctga cgagaagatc
     2101 gcgcgggcga tcgctacgtg cggaacgaga aggacacctg gtgcaggacg agacgagcga
     2161 gaaggacgag aagaagcagc ccaagggcga ggaggtcgca gactccaacc ctgtacctcg
     2221 cgacgacgcc ctcgactcgt tctggagcgt tacccggcca gacccaggtc cgctcctgcc
     2281 cctcttcgaa cctcctgaac ccatccccga accggagatc gagccggtct tcagttggac
     2341 gatggagtcg gagtctggcg gcggggccgc cttcgctgaa cccgagccgc tcgtcgaaga
     2401 gcgcagtgat actgcggtgc ctgaacccgc ggccgggcaa gaagccgaag ctccgcgaag
     2461 cctctggtgg gagcagcccg agccatccct ggaagacatc ctgctcgaac cccagagcac
     2521 ttcggccgag gt
//
LOCUS       DATDOZ010000224         3823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_970088, whole genome shotgun sequence.
ACCESSION   DATDOZ010000224 DATDOZ010000000
VERSION     DATDOZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970088"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="rsmH"
                     /locus_tag="VI541_04515"
     CDS             <1..777
                     /gene="rsmH"
                     /locus_tag="VI541_04515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLF70199.1"
                     /translation="DALGAAAERLQRFGARFKPVRANFAHLGRVIREQGYGEVAAVLF
                     DLGVSSPQLDRSDRGFSYRDDGPLDMRMDQQADLRAADVVNGYSQERLTQILFEYGEE
                     SQARRIARAIVRRRAQAPLTGTAELAEVVRAAIPAAKRRTGGHPARRSFQALRIEVNA
                     ELDSLKAALPAAVEVTAPGGRVVAISYHSLEDRIVKRTFADYARGCTCPRDLPICVCG
                     KEATVRVLTKRAIRPSPTESGSNRRSESARMRVAQKLVAA"
     gene            777..1244
                     /locus_tag="VI541_04520"
     CDS             777..1244
                     /locus_tag="VI541_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70200.1"
                     /translation="MSSVPRQIARPRTQPRRRPVTGRPLLRVIDGGRASAADAKRRST
                     IIFLTWSGVAGAALIFGVVLLNVFVAQGSFKLEDLQSQVEQQSNEYRRMRFEVAKAEA
                     PARVAEAATALGLVVPDTQEYILGLPVEHPAQVAEKALSGDQLALKAGLDGEP"
     gene            1325..3055
                     /locus_tag="VI541_04525"
     CDS             1325..3055
                     /locus_tag="VI541_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLF70201.1"
                     /translation="MFVVFMLMLVALTTRLVFVQGLDSRHFEALAADQRERRLVLPPQ
                     RGSIYDRNGAELAMSLDMQTIFANPHFISDPGAAASALAPILGQSEESIRSRLSRKAG
                     FVYLARKVEPSVATAVKGLQIQGVDTVPESKRFYPAGGLASHVLGFVGTDNEGLAGME
                     MRFEKLLKGLPGALTMESDPTGRPIPAGHFSRTAPTAGSDIILTLDREIQYQTEVFLA
                     QAVQTYKAKAGSAIVMDPRTGDILALANVPTFDPNDVASSSGESRRNRALVDVYEPGS
                     ANKIITASAAIGSGVVKPDDMFKVPDNLKLGSKVFHDAHPHAVQDLSFAQIIEQSSNV
                     GTIKVAALLGKERLYSALSRFGYGKPTGLDFPGESSGILTPPAKWSTPSMGTVPIGQG
                     VAVTVMQIVNVYATVANHGVHVQPRLVRALVDESGKPRYTPDGARREVVDAKTADTVT
                     KILIRVTEGEHGTGKAAAVPGYQVAGKTGTAQKPIPGGGGYSGYVGSFIGFAPAGDPR
                     LVVGVVLDDPSPIWGGVTAAPTFKQIMQFALRHLGIGPGSVLQTEGTPLPTPVRSGGA
                     PAPAPSPPSD"
     gene            3085..>3823
                     /locus_tag="VI541_04530"
     CDS             3085..>3823
                     /locus_tag="VI541_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLF70202.1"
                     /translation="MKLSLVSTLLGGPQTTSDIEVTDLAYDSRHVGGGSLFFCISGRS
                     FDGHDFAQDAISGGAIALVVERPLDIDVPQIVVPQVRDAMTAFAAPFYGTPSNDLKVL
                     GVTGTNGKTTTTFMAHSIFRTMGDISGLIGTVETRVGDTIAAGVRTTPESIDIQRLLR
                     SMVSEGARSVAMEVTSEGMAERRLVGTRFRCSVFTNLSQDHLNYHGSMESYFSAKARL
                     FSSDYSDSACVNIDDGYGARLAAQISIP"
BASE COUNT          691 a         1177 c         1255 g          700 t
ORIGIN      
        1 gatgccctgg gtgcggcggc ggagcgtttg cagaggttcg gcgcgaggtt caagcccgtg
       61 agggcgaact tcgctcatct gggcagggtg atcagggagc agggatatgg agaggttgcg
      121 gcggtcctat tcgatctggg ggtctcgtct ccccagcttg accgttccga taggggtttc
      181 agctatcggg atgacggtcc tctggatatg cgcatggacc aacaggcgga tcttcgagcg
      241 gcggatgtcg tgaacggcta ctcgcaggag cgactgactc agatcctctt cgagtacgga
      301 gaggagtcac aggcccggcg cattgcacgc gccatagtcc ggcgccgtgc ccaggcgcca
      361 ctcacgggga cggccgaact ggcggaggtt gtacgcgccg cgatcccagc agccaagcgc
      421 cgcacgggtg gccaccctgc ccgcaggagt tttcaggctc ttcgcatcga ggtgaacgcg
      481 gaactcgatt cgctcaaggc tgccttgcct gccgccgtcg aggtcacggc ccccgggggc
      541 cgtgtggtcg cgatcagtta tcactcgctc gaggaccgga tcgtcaagag aacctttgcc
      601 gactacgcca gaggctgcac ctgccctcgc gacctcccca tctgtgtctg cggcaaggag
      661 gcaacggtcc gggtgctgac gaaacgcgcg atacgaccat cgccgacgga gtccggatct
      721 aaccgtcgct cagaaagcgc ccgcatgcgc gtggcccaga aactggtagc cgcctgatgt
      781 ctagcgtccc ccgccagatc gcgcgcccgc ggacgcagcc acgccgccgg ccagtaaccg
      841 gcaggcccct gctgcgtgtc atcgatgggg gccgcgcatc ggccgccgat gcgaagcggc
      901 ggtcgacgat catcttcttg acatggtcgg gggtggctgg tgctgccctg atatttggag
      961 tcgtgctgct taacgtcttc gtggctcagg gatctttcaa gcttgaagac cttcagagcc
     1021 aggtcgagca gcagagcaac gagtatcgcc gcatgcgatt cgaggtggcg aaagctgagg
     1081 cccccgctcg cgtggccgaa gctgcaaccg ctctcggttt ggtcgtacct gatacgcagg
     1141 agtacatcct cggacttccc gtggagcatc cggctcaggt ggccgagaag gccctttcgg
     1201 gcgatcagct ggccctaaag gccgggctcg atggagaacc gtagatagga ccgcaggatc
     1261 ggctctagag gggagactgg tcaggatgag caagacgaac agatcgagct ccaggctcgt
     1321 gagtatgttc gtcgtcttca tgctcatgct cgtcgcgctg accacccgcc tcgtcttcgt
     1381 acaggggttg gactcacggc acttcgaagc gttggcggca gatcagcgtg agcgcaggtt
     1441 ggtccttccc cctcagcgcg ggagcatcta cgaccgcaat ggcgcagagc tggcgatgtc
     1501 gctggacatg cagacgatct tcgccaatcc gcacttcata tccgacccgg gggctgctgc
     1561 gagcgctctc gctccgatac tcggacagag cgaggagtcg atcaggagcc gtctgagccg
     1621 caaggccggg ttcgtctacc tcgcgaggaa ggtcgaaccc tcggttgcga cagcggttaa
     1681 gggtctgcag atccagggcg ttgacacggt cccagagtcg aagaggttct atccagcagg
     1741 agggttggcg tcgcatgtcc tcggtttcgt tggaaccgac aacgagggcc tggcgggcat
     1801 ggagatgcgc ttcgagaagc tcctcaaggg gttgcccggt gcattgacca tggagagcga
     1861 cccgacaggt cgcccgatcc ctgcgggtca cttctcacgc acggccccca ccgcgggttc
     1921 cgacatcatc ctcaccctgg atcgcgagat ccagtaccag acagaggtct tcctcgctca
     1981 ggcggtgcag acctacaaag ccaaggccgg ctcggccatc gtcatggatc cacgcaccgg
     2041 agacatcctg gccctggcca acgtcccgac cttcgacccg aacgacgtcg cttcgagttc
     2101 tggggagtcc cgtcgcaatc gggctctggt ggacgtctac gagccgggct cggccaacaa
     2161 gatcatcacc gcgtcggcgg cgatcgggtc gggtgtcgtg aagcccgacg acatgttcaa
     2221 ggtcccagac aatctgaaac tgggtagcaa ggtctttcac gacgctcacc cgcacgcggt
     2281 ccaggatctc tcgttcgctc agatcatcga acagtcgtcg aacgtcggaa ccatcaaggt
     2341 ggcggcgctg ctcgggaagg agcgcctgta cagcgctctt tcccgcttcg ggtacggcaa
     2401 acccacaggg ctcgacttcc cgggggagtc ctcgggcatc ctgacgccgc ctgcgaagtg
     2461 gtcaacgccc tcgatgggga ccgtcccgat cggacagggg gtggctgtaa cggtgatgca
     2521 gatcgtgaac gtctacgcga cggttgcgaa tcatggcgtg cacgttcagc ccagacttgt
     2581 gcgcgctctc gtcgacgaga gtgggaagcc ccgctacaca ccggacgggg ctcggcgcga
     2641 agtcgtcgat gcgaagacgg cagataccgt gaccaagatc ctgatccggg tcacagaggg
     2701 ggaacacggg acagggaaag cggcggctgt ccccggatat caggtcgctg gcaagaccgg
     2761 aaccgcacag aagccgatcc caggcggcgg tggctactcg ggttacgtcg gttccttcat
     2821 cggcttcgct ccggccgggg accctcgtct ggtggttggg gtggttctcg acgatccttc
     2881 tcccatctgg ggaggcgtta ctgccgctcc gaccttcaaa cagatcatgc agttcgcgct
     2941 gaggcacctc gggatcggcc cggggtcggt ccttcagact gaaggaacac ccctcccgac
     3001 cccggtccgt tccgggggtg ccccggcacc agcgccgagt cccccgtcag actagggggc
     3061 cctggatgat gggatgctga gtctatgaag ctcagcttgg tgagtaccct cctggggggt
     3121 ccgcagacga cctccgacat cgaggtcacg gacctggcct atgactcgcg tcacgtggga
     3181 ggagggtccc tcttcttctg catctcgggt aggagtttcg acgggcatga cttcgcgcag
     3241 gacgcgatca gcgggggagc gatcgccctc gtggtcgaga ggccactcga catcgatgtg
     3301 ccgcagatcg tggttccgca ggtacgcgat gccatgactg ctttcgctgc ccccttctac
     3361 gggacaccgt cgaacgatct gaaggtgctg ggggtcaccg gcacgaatgg gaagacgacg
     3421 acaacgttca tggcgcactc gatattccga acgatgggcg acatctcagg tctcatcggg
     3481 accgtcgaga cgcgcgtcgg cgacaccatc gcggctgggg tcaggacgac tcccgagtcg
     3541 atcgacatcc agaggctgct gcgaagcatg gtctccgagg gggccaggag tgtcgcgatg
     3601 gaggtgacct cggagggcat ggccgaacga cggcttgtgg gtacccgttt ccgttgcagc
     3661 gtcttcacga atctgagcca ggaccatctg aactaccacg ggagtatgga gtcctatttc
     3721 tcggccaagg cgcggctctt ctcgtccgac tactcggatt ccgcctgtgt caacatcgac
     3781 gatgggtatg gcgccaggct cgccgcgcag atttcgatcc cct
//
LOCUS       DATDOZ010000225         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_189148, whole genome shotgun sequence.
ACCESSION   DATDOZ010000225 DATDOZ010000000
VERSION     DATDOZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189148"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="truB"
                     /locus_tag="VI541_04535"
     CDS             <1..915
                     /gene="truB"
                     /locus_tag="VI541_04535"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HLF70203.1"
                     /translation="KPSGCTSHDVVARIRRVVAEKRVGHAGTLDPSATGVLIIGVGRA
                     TRLLQFIQHLPKTYIATARFGVTTTTQDADGEVIAQRPFEDSLGGLTAVLDDYRGEIK
                     QIPPMVSAVKVGGEPLYRAARRGEEVPRPPRAVHVYDLIIDDLDAAAGTATFTVTCSS
                     GTYVRTLVADIGEAIGCGAHLIELRRTSIGSFTVSECTPLAVIEQAPQEHPLLSMAEA
                     MRDFPNLTVSGAELDDAIHGRPITADLQPSRAGEIHVISAPRAGQRPAHEVGMSAGIP
                     VAILDTQGTLRAVYRKNVKGFVAAAVFE"
     gene            912..1838
                     /locus_tag="VI541_04540"
     CDS             912..1838
                     /locus_tag="VI541_04540"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HLF70204.1"
                     /translation="MRVYRGLSELPRRGSPSAVTIGNFDGVHLGHVTMLRRLLLRAEE
                     SGAIPTVLTFSPHPQSVLHGEAPPSLATPKRKLELLAEHGVKRIVILKFNKKFSLTEP
                     EEFISRILIDELRTREIVVGANFRFGRMARGDVTMLKSAGHESGFVVDGVRLAELEHR
                     TLSSTEIRHALAAGDIDWANKALGRPHSVPGKVVSGAGRGKGLGYPTANLRPEPGFCL
                     PATGIYAGRVRLRDRIMPAAMSVGHNPTFGRNPLSVEAFVLDFDGTLRSRHVEFEFVS
                     RIRDEAKFETAAALSAAIAADVAEVRRLLHIR"
     gene            1870..>2108
                     /locus_tag="VI541_04545"
     CDS             1870..>2108
                     /locus_tag="VI541_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70205.1"
                     /translation="MPTWLLSLKVASSPTARRSKNASSSSLSLLILFTTLSNPSRASA
                     EGTLLVNVIVGVVMGDYGPIQAADVYVTLLGSAVQ"
BASE COUNT          405 a          656 c          643 g          404 t
ORIGIN      
        1 aaaccctcag gctgcacatc tcatgacgtc gtcgcgcgca tacgaagagt cgtcgccgag
       61 aaacgagtgg gtcatgcagg aactctggac cctagcgcaa cgggagtcct gatcatcgga
      121 gtgggtcgtg cgacgcgact gctccaattc atccagcatc tccccaagac ctacatcgcc
      181 accgctcgtt tcggcgtgac cacgacaacg caagacgccg atggagaagt gatcgcgcag
      241 agaccattcg aagactccct cggcggactg acagccgtcc tcgatgacta ccgaggggag
      301 ataaagcaga tcccgccgat ggtctcggcg gtcaaggtcg gcggcgagcc tctctaccgc
      361 gcagcgaggc ggggggagga ggtaccaagg cccccgagag cggtccatgt ctatgacctg
      421 atcatcgatg atctcgatgc cgccgcgggg acggccacgt tcacggtcac ctgttcgagt
      481 ggtacctatg tccgcacgct cgtcgccgac atcggtgaag cgatcgggtg tggtgcccac
      541 ctgatcgaac tgaggaggac ctctatcggc tccttcactg tttccgagtg cactcctctc
      601 gccgtgatcg agcaggctcc ccaggaacac cccctgctct cgatggccga ggcgatgagg
      661 gacttcccga atctcacggt ttcgggggcc gaactcgacg acgctattca cgggcgacca
      721 atcactgcgg accttcaacc ttctcgcgct ggagagatac atgtcatatc cgcaccgcgg
      781 gcgggtcagc gaccagccca tgaggttggg atgtccgcgg ggatccccgt tgcgatcctc
      841 gacacgcagg gaacgctccg ggcggtctat cgaaagaatg tcaaaggctt cgtggccgcg
      901 gcggtgttcg agtgagggtc tatcgcgggc tgagcgaact gcctcgccga ggctccccct
      961 cggcggtgac catcggcaac ttcgatggag tgcatctcgg tcatgtgacg atgctacgca
     1021 gactcctgtt gcgcgcagag gagtccgggg caatacctac ggtgctcaca ttcagccccc
     1081 acccacagtc ggtgttgcat ggtgaagcgc caccgtcgct agcgacaccg aagcgaaaac
     1141 tcgaactgtt agcggaacac ggcgtgaagc gcatcgtgat cctgaagttc aacaagaagt
     1201 tctccctaac tgaaccggaa gagttcatca gcaggatctt gatcgatgag ctgcgcaccc
     1261 gagagatcgt tgtcggtgcg aactttcgct tcggccgtat ggcccgcggt gatgtcacga
     1321 tgctgaagtc agcgggtcac gagagcgggt tcgtcgtcga cggggtccga ctggccgagc
     1381 tcgaacatcg cacgctgtcg agcacggaga tccgccatgc tctggccgcg ggagacatcg
     1441 attgggcgaa caaggccctc ggtcgtcctc acagcgttcc gggcaaggtg gtgtcggggg
     1501 ccgggcgcgg caaagggctc ggctatccga ccgcgaacct gcgccccgag cctgggttct
     1561 gtctgcctgc caccgggata tatgcaggac gtgtgcgcct gcgagaccgg atcatgcccg
     1621 cagcaatgag tgtgggccac aaccccactt tcggcagaaa cccgctgagc gtcgaggcct
     1681 tcgtgctcga cttcgatggg acccttcgtt cgcgccacgt cgagttcgag ttcgtttctc
     1741 gcatccgcga cgaagccaag ttcgaaacgg ccgcagccct ctcagcggcg atcgccgccg
     1801 atgtcgccga ggtccgccgt ctccttcaca tccgctgacg gcggacgttc ctcagggacg
     1861 atcccaacct tgccaacctg gctcctatcc cttaaggtcg cctcgagtcc aactgccagg
     1921 aggtcgaaaa atgcgagctc gtcttctctc tctctcctaa tcctgttcac cacgctctcg
     1981 aatccgagca gagcttctgc tgagggtacc ctgctggtaa acgtaatagt gggtgttgtt
     2041 atgggtgact acggcccgat acaggcggca gatgtgtatg tgactcttct tggttcggcc
     2101 gtgcagga
//
LOCUS       DATDOZ010000226         4779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2544368, whole genome shotgun sequence.
ACCESSION   DATDOZ010000226 DATDOZ010000000
VERSION     DATDOZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544368"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VI541_04550"
     CDS             complement(<1..234)
                     /locus_tag="VI541_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70206.1"
                     /translation="MIFASGAVLSFLPWAWRGAARSWRHLLFAGFAIGAIAIGVATRG
                     PLVVPLIAVMASSVGLWLGARRGEISFRVVHLAL"
     gene            complement(231..827)
                     /locus_tag="VI541_04555"
     CDS             complement(231..827)
                     /locus_tag="VI541_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70207.1"
                     /translation="MPQARSLLAVHLFAVVGGVSIALGFAPGGPLALMGALVAMTWTR
                     HSLGRRAPSVWSVSSVGVLIALIALSFERYSANEVASLMGAQAAIGPLVGGSSLRLLV
                     ILAGVHLVGLVAAVTWLGSSPRTDRVSTLSMDIFGATLAGTVITGVLWGPPIAAIFLG
                     PARLTGPSLVTLGISLISSAAIVIAVAPTRRRFFREQT"
     gene            complement(832..1245)
                     /locus_tag="VI541_04560"
     CDS             complement(832..1245)
                     /locus_tag="VI541_04560"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HLF70208.1"
                     /translation="MQGLTDFRLARRAVIEGFKRGSLDRNEICDANPELLRAAKNIGK
                     ACDRPCPICGQDALRQVRYVYGDDLKQLSGRPVYPEGWADQLAAEHDEFRCYSVEVCV
                     ECSWNHLAACYLMGRRYAVAGERRRLGRARAGRPR"
     gene            1371..1901
                     /locus_tag="VI541_04565"
     CDS             1371..1901
                     /locus_tag="VI541_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLF70209.1"
                     /translation="MAVLGLLKDRSMHGYELKKELTAQLGQFWQFSYGSLYPTVKRLE
                     KSGAVERIFPKEDVKRRLNIYRITSIGEEMFTKALGEHTAVDDARFSMKLAFFRYLKP
                     VDRVEVLERRRAYLTEKLAEMRSKLRTYRERIDAYTLQLMEHGVDTTTADIEWIDRLL
                     AEEKASEKASPRRATT"
     gene            1916..3046
                     /locus_tag="VI541_04570"
     CDS             1916..3046
                     /locus_tag="VI541_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HLF70210.1"
                     /translation="MSNKIRIGIIGAGNCASSLIQGIEFYRDARDGDDVPGLMHVRVG
                     SYHVSDVEVSCAFDVDANKVGVDLSKAIQAPPNNTIQFAKVPDLDVTVQRGPTLDSLG
                     LYYREEITESPAAPVDVARVLRETNTQVLINYLPVGSEKAAKFYAEQALDAGVAFINC
                     MPVFIASHPAWASKFAERGIPIIGDDIKSQVGATIIHRMLAKLFEDRGVKLDHTYQLN
                     FGGNMDFKNMLERERLVSKKKSKTQSVTSQLRKGIDSKDVHIGPSDHVPWLEDRKWAH
                     IRLEGREFGDIPLSVDLKLEVWDSPNSAGIVIDAIRCLRLALDAGVGGPLFGPSAYFM
                     KSPPVQHSDDVARQMVEEFIHSVGLPEADLRIFEQSAEIGLN"
     gene            3098..3610
                     /locus_tag="VI541_04575"
     CDS             3098..3610
                     /locus_tag="VI541_04575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLF70211.1"
                     /translation="MVSFAVFKTNKGTIRVKLLPETAPKTVENFTGLATGTKEWTHPE
                     TGESKAVSLYAGTIFHRVIPNFMIQGGDPLGRGTGGPGYKFEDEVGAGPSFDRKGYLA
                     MANAGPNTNGSQFFITVAPTPHLTGKHTIFGEVVEGQEVAEAISRVPTGPGDRPQEDV
                     VLESIVIEQG"
     gene            complement(3812..4207)
                     /locus_tag="VI541_04580"
     CDS             complement(3812..4207)
                     /locus_tag="VI541_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HLF70212.1"
                     /translation="MGMTVSDLGRRVGVKADTVRYYERIGLLDPPDRTRSGYRSYPDE
                     ALDRLRFIKEGQRVGLQLSDIKELLDVLDRGQCPCGHTLTLVERRLAQVRAEIEKLKH
                     VEERLMQMACCPQSSSIWCCPDDSVAERG"
     gene            complement(4254..>4779)
                     /locus_tag="VI541_04585"
     CDS             complement(4254..>4779)
                     /locus_tag="VI541_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF70213.1"
                     /translation="AQLGTLEQVAMSPFGLSKVVLAEVAAGLVFQFMMLAVLLVCMMA
                     VTGKWLHMDLLSIIPIALMTVGGVVGLSMVMGGLAVVFKRVQSALQIMQMVFIALVAI
                     PVSHFTWIKYMPLAWGNSLLRRVMVNGDSLLTIPVRDSLFLFAHMVAYLVVGTIVFRY
                     FERIARARGLLGHY"
BASE COUNT         1049 a         1506 c         1420 g          804 t
ORIGIN      
        1 gagagcaaga tggaccactc ggaatgagat ctcgccccgg cgcgccccga gccagagccc
       61 cacactgctc gccatgaccg cgatcagcgg gaccacaagg gggccccgag ttgcaacacc
      121 gatggcgatg gcacctatgg caaaacccgc gaagagcagg tgccgccagg accgtgcagc
      181 tccacgccag gcccacggga ggaaagagag caccgcccca ctcgcgaata tcaagtctgc
      241 tcccggaaga agcgccgcct ggtcggagcg accgcgataa cgatcgccgc gctgctgatc
      301 agcgagatcc ccagagtgac gagcgagggc cccgtcaacc tggcgggacc gaggaagatc
      361 gccgcgatgg gcggtcccca tagaacccca gtgatgaccg tccccgcaag cgtcgcgccg
      421 aagatgtcca tcgaaagcgt tgaaacccgg tcggtgcggg gcgatgagcc tagccaggtc
      481 accgcggcga ctaggcccac gaggtgcact cccgcgagga tcaccaagag cctcaaagag
      541 gatccgccga ccagggggcc gatggcggcc tgggccccca tgagcgacgc cacctcgttc
      601 gcgctatagc gctcgaagga aagggcgatc agcgcgatga gcacgcctac cgaagagacc
      661 gaccagacag aaggagcgcg cctgcccagg gagtgtcgag tccaggtcat ggctaccagt
      721 gcccccatca gagcgagcgg gcccccaggg gcgaacccaa gggcgatgga aaccccgccg
      781 acgacagcga aaagatggac ggcgagtagc gacctagcct gaggcaaagc cctatcgggg
      841 acggccggcc ctcgcacggc ccaggcgacg tcgttccccc gcaacggcgt atcttcgacc
      901 catcaggtag caggcagcca ggtggttcca ggagcactcg acacagactt ccacggagta
      961 gcatcggaat tcatcgtgct ccgccgcaag ctgatccgcc caaccttccg ggtagacggg
     1021 gcggcccgat agctgcttga gatcatcacc atagacatac cgcacctgcc tgagggcatc
     1081 ctgcccgcag atcgggcagg ggcggtcgca ggccttgccg atgttcttcg ccgcacgcag
     1141 cagctcaggg ttggcgtcgc agatctcgtt tcggtcgagg ctcccgcgct tgaacccctc
     1201 gatcacggcc cttctggcca ggcgaaagtc ggttagcccc tgcataacct gacaatcata
     1261 gccccggccc tcctgaaggc cccccggatg gggcttcttg acggatgggc tggctcgcgc
     1321 ttagatatat cgacgcgata tatcgaggag atacctttca agtgatcgaa atggcagtcc
     1381 tggggcttct caaggaccgt tcgatgcacg gttacgagct caagaaagag ctcaccgcgc
     1441 agctcggaca gttctggcaa ttctcctatg gcagcctcta cccgaccgtt aagcgcctcg
     1501 agaagtcggg ggcggtcgag cgtatcttcc cgaaggagga cgtcaagcgc aggctaaaca
     1561 tctaccgcat cacctcgatc ggcgaggaga tgttcaccaa ggccctcggc gagcacaccg
     1621 ccgtcgatga tgctcgcttc agcatgaaac ttgccttctt ccgctatctg aagcccgtcg
     1681 accgcgtcga ggtactggag cgccgcaggg cttacctaac ggaaaaactc gcggagatgc
     1741 gatccaagct tcgcacctat cgagaacgca tcgatgcata caccctgcaa ctgatggagc
     1801 acggggtaga caccacaaca gctgacatcg aatggataga ccgtctgctc gctgaagaga
     1861 aggcatccga gaaggcctcg ccccgtaggg cgaccacctg agtaaggaga agactttgag
     1921 taacaagatc cgcatcggga tcatcggtgc aggcaactgc gcctcgtcgc tgatacaggg
     1981 catcgagttc tatcgtgatg cgcgcgacgg tgacgatgtt ccgggcctta tgcacgttcg
     2041 ggttgggtct tatcacgtct cggatgtcga ggtctcgtgc gcgttcgacg tcgacgcaaa
     2101 caaggtcggg gtcgatctga gcaaagcgat ccaggccccc ccgaacaaca ccatccagtt
     2161 cgccaaggtc cccgacctcg atgtcacggt gcaacgcggc ccgaccctcg atagcctggg
     2221 tctctactac cgcgaagaga taaccgagtc tccggctgcc cccgtcgatg tcgcgcgggt
     2281 tctccgtgag accaacactc aggtgctcat caactatctt ccggtcggca gcgagaaggc
     2341 ggccaagttc tacgcggagc aggctctcga tgccggagtc gccttcatca actgcatgcc
     2401 ggttttcatc gcatcgcacc ccgcatgggc ttcaaagttc gcagagcggg ggatcccgat
     2461 catcggcgac gacataaaga gtcaggttgg cgcaacgatc atccaccgca tgctcgcgaa
     2521 gctgttcgag gaccgcggcg tgaagctgga tcacacctac cagctgaact tcggcgggaa
     2581 catggacttc aagaacatgc tcgagcgcga gaggctcgtc tcgaagaaga agtccaagac
     2641 ccagtcggtc acttcacagc tacgcaaggg tatcgactcc aaggatgtcc acatcggtcc
     2701 atctgatcac gtgccttggt tggaggatcg caagtgggct cacatccgcc tagaagggcg
     2761 cgagttcggt gacatccctc tctctgttga tctgaagtta gaggtctggg actcacccaa
     2821 ctctgcaggg atcgtcatcg acgctatccg ctgcctgcga ctggcactcg atgctggcgt
     2881 cggggggccg ctcttcgggc caagtgctta cttcatgaag tcgccgccgg ttcagcattc
     2941 cgatgatgtg gctagacaga tggtagaaga gttcatccac tcggtggggc tccccgaggc
     3001 ggacctgcga atcttcgagc agagcgctga gatcgggctc aactgatctt cgggaagacg
     3061 tttagactgc gggctccaag taagcaggag ggatccgatg gttagtttcg ccgtgttcaa
     3121 aacgaacaag ggcaccatcc gcgtgaagct gcttcccgag accgccccca agacggtcga
     3181 gaacttcacc ggcttggcga ctggtaccaa ggagtggacg caccccgaaa ctggcgagtc
     3241 aaaagcagtc tcgctctacg ccgggacgat ctttcaccga gtgataccca acttcatgat
     3301 ccagggcggc gatccgctcg gtcgcggcac cggcggccca ggctacaagt tcgaagatga
     3361 agtcggagca ggaccgagct tcgaccgcaa ggggtacctc gcgatggcca atgccgggcc
     3421 caacaccaac ggtagccaat tcttcataac ggtcgcgccg actccgcacc tgacgggcaa
     3481 gcacacgatc ttcggtgaag tggtggaagg gcaagaggtc gccgaagcca tctcccgggt
     3541 tccgactggc cccggcgacc gcccacaaga ggacgttgtc ctcgaaagca tcgttatcga
     3601 gcagggctaa gaggccggat aagcctcaca gcaacccggc caacagcaga ggctctccgc
     3661 aactggcggt gccgccactc cgggggcctg gctggcacct ctataactgg cgccgccctc
     3721 accgcccatc ttcgactcct tggttgggga gcagaatggg cactcgccaa gtgcacacac
     3781 ctcgtcgatc tcagcgatca atctcataca ttcaccccct ctcggccacg gaatcgtccg
     3841 gacagcacca gatactcgat gactgcggac agcaggccat ctgcatcaaa cgttcctcga
     3901 catgcttgag tttttcgatc tcggcccgga cctgggcaag gcgcctctcg acgagggtca
     3961 gagtgtgacc acaggggcac tgcccccggt cgaggacatc gagcagctcc ttgatgtcgg
     4021 agagctgaag ccccactcgc tgaccctctt tgatgaaccg gagccggtcg agggcctcat
     4081 ctggatagct tcggtacccg gatcgggttc ggtcgggtgg gtccaagaga ccgatgcgct
     4141 cgtagtagcg caccgtgtcg gccttcacgc cgacccgtcg ccctagatca gagactgtca
     4201 ttcccataac ggaagcagtg taaacctttg agtcaactcc aaggtcaagt cttctagtag
     4261 tgaccgagga ggccccgggc cctcgcgatc cgctcgaagt agcggaagac gatagtccct
     4321 actaccaggt aggcgaccat gtgtgcgaag aggaagagcg agtcacggac gggaatcgta
     4381 agaagagaat caccgttcac catcacgcga cgcagcagcg agttgcccca tgcgagcggc
     4441 atgtacttga tccaggtgaa gtgcgatacg ggtatcgcga ccagcgctat gaaaaccatc
     4501 tgcatgatct ggagcgccga ctgcacgcgc ttgaagacga cggcaaggcc ccccatcacc
     4561 atcgacagac ccaccacacc gccgaccgtc atcagcgcga tcgggatgat gctcagaagg
     4621 tccatgtgca gccatttgcc tgtcaccgcc atcatgcaga ctagaaggac cgccagcatc
     4681 atgaattgga agacaaggcc cgccgccacc tcggcaagca cgaccttgct gagaccgaat
     4741 ggcgacatcg ccacctgctc gagcgtgccg agttgtgct
//
LOCUS       DATDOZ010000227         3964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2816642, whole genome shotgun sequence.
ACCESSION   DATDOZ010000227 DATDOZ010000000
VERSION     DATDOZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816642"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VI541_04590"
     CDS             <1..523
                     /locus_tag="VI541_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF70214.1"
                     /translation="LAIDTPEKVGRLVLVGANPGIVDETKRHARRTADERWIRLLREE
                     GLDVFLAKWLVQPMFGGFTQEEIIEMGQKRSRSDSEALASSLELAGVGIMAPMWERLR
                     DIAHETLIIVGSKDRKFSAIADQMAGKIPNSQIASIAGAGHAAHLEDPIAFGRLVREF
                     LEGHVATPDATSQ"
     gene            complement(490..1359)
                     /locus_tag="VI541_04595"
     CDS             complement(490..1359)
                     /locus_tag="VI541_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLF70215.1"
                     /translation="MRGFRIPLAEPFRGQTHRTGFVVEGRTGWAECSPFPGHSDPVVC
                     EGSARSVADESWPAPVRDRVAVCALVPACDPRRAARIVAESGCSGAKVKVGDPGSVAR
                     VAAVRGALGDDGKIRIDANGAWEVEKAAEMIRSLSAFNLEFVEQPVATLAGFARLKDL
                     VDVPLAADELIVSPETFRHCARSGVVDVLVARVQHLGGVNAALELINAAGLPVVVSSL
                     IETSIGLSAGVALAAAMPELHHASGLGTAAMLSGDIVDEPLLPSEGWLQVRRPEVNEE
                     KLRYWEVASGVAT"
     gene            complement(1356..2219)
                     /locus_tag="VI541_04600"
     CDS             complement(1356..2219)
                     /locus_tag="VI541_04600"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HLF70216.1"
                     /translation="MPSVWLEAIRPKTLSVGVVPVLVGTSAAHEFHAWRVVGALVIAV
                     TLQIGVNLANDYFDGRRGVDTPERTGPRRAVASGLVAPAAMRRAMLGTFATAGIVGSV
                     LAWQVSWLLLPVGALAIISAVLYSGGPRPYASAGLGEVFVFIFFGVVATVGSTFIQDL
                     TVSAVALMASIPVGLLAVAVLMANNLRDIPTDTDAGKMTLAVRLGEHRTRLAFIAILV
                     VSVLMALPVAMLRQSWLPALSLGALPLILRPAQLVWTRSGRDLIPALVGTARLQLVFG
                     LLLSLGLAMSR"
     gene            complement(2227..3057)
                     /gene="menB"
                     /locus_tag="VI541_04605"
     CDS             complement(2227..3057)
                     /gene="menB"
                     /locus_tag="VI541_04605"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HLF70217.1"
                     /translation="MENPYQPAWKASGEYEDIIYEVWDGIAKITINRPEVRNAFRPKT
                     LFELSRAFDVARDDPTVGVIIFTGAGDLAFCSGGDQRVRGNDGYLDEKGIGRLNVLDL
                     QVQIRRLPKPVIAMVAGYAIGGGHVLHIVCDLTIAGHNARFGQTGPKVGSFDGGYGAG
                     LLARIVGHKKAHEIWYLCREYDAQQALDMGLVNTVVPLEDLERETVAWARRMLEMSPL
                     ALRLLKASFNADTDGLAGIQQLAGDATLLYYMSEEAQEGRDAYVQRRRPEFERFPKRP
                     "
     gene            3117..>3964
                     /locus_tag="VI541_04610"
     CDS             3117..>3964
                     /locus_tag="VI541_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLF70218.1"
                     /translation="MTQGALVAVDTTDPIAFYRQMTTCWENGDAVLPVDHRLSRAGAR
                     ALIDRFKPAYFIDQRRVIGLAGGVPVSPGIALVVATSGTTGEPKGVELTHANLAASAY
                     ATARWLGTGPEDRWCCCLPLAHIAGISTLTRSIMADAEPEFTTFGVDEILRTKATCIS
                     LVATMLARLIEAEVDLTRFRVILVGGGPTPRSLIEIAQRSGARVVATYGMTETTGGCV
                     YDGNALPGVDIEIGAHGVIRLHGPMVMSGYRLDEMGTERSLQDGWFETSDLGSLDSGK
                     LTVLGRR"
BASE COUNT          826 a         1281 c         1178 g          679 t
ORIGIN      
        1 gcttgcgatc gatactcctg aaaaggtggg gcgactcgtt ctcgttgggg cgaaccctgg
       61 gatcgtcgat gagacgaaga gacatgcacg ccgcactgcc gacgagaggt ggatacgtct
      121 cttgcgcgaa gagggcctcg atgtgttcct cgcgaagtgg ctcgtccagc cgatgttcgg
      181 cggtttcacg caagaagaga tcatcgagat gggtcaaaag cgaagccgga gcgactccga
      241 ggccctcgcg tcgtcgctcg aactggccgg agtgggaatc atggccccga tgtgggaacg
      301 tctccgtgac atcgcgcacg aaacactcat catcgtggga agtaaagacc gcaagttctc
      361 tgcgatcgcc gatcagatgg ctggcaagat cccgaactcg cagatcgcaa gcatcgcggg
      421 ggccgggcac gcggcccacc tggaagaccc catcgcgttc ggaaggttgg tgagagagtt
      481 cctcgaaggt cacgtggcaa cccccgatgc aacttcccag tagcgcagtt tctcctcgtt
      541 gacctcgggt cttctcacct ggagccaccc ttcactcggg agcaggggtt catcaacgat
      601 gtccccggat agcatcgccg cagtacccag tccggatgcg tgatgcaact cgggcatcgc
      661 cgcagccagt gcgactccgg ccgacaaccc gatcgatgtc tcgatcaaag aagacacgac
      721 caccggcagc ccagctgcgt tgataagttc cagcgccgca ttcactccgc ccagatgctg
      781 cacacgggcg accaaaacat cgacgacccc cgatcgcgcg cagtggcgga atgtctccgg
      841 cgaaacgatc aactcatcgg ccgcgagagg aacatcgacc agatccttca aacgagcgaa
      901 ccccgcaagg gtcgcgaccg gctgctccac gaactcgagg ttgaaggcgc tcagagaccg
      961 gatcatctcc gctgccttct cgacctccca agcaccgttg gcatcgatgc ggatcttgcc
     1021 gtcatcaccg agggcccctc gcaccgccgc aacccgcgcg acactaccgg ggtcccccac
     1081 cttcaccttc gcgccgctac atccggactc tgcaacgatc cgagcggctc tgcgtggatc
     1141 gcatgctggg accaaggcac agacggcaac gcgatcacgc accggcgcag gccatgactc
     1201 atcagcgacg ctacgagccg aaccctcaca gaccacaggg tccgaatggc ccgggaatgg
     1261 agagcactcc gcccaacccg ttcggccctc gaccacgaag ccggtcctat gggtctgacc
     1321 tcggaagggc tcggccagcg ggatacggaa tcctctcatc gtgacatcgc aaggccgaga
     1381 gagagcagca gaccgaacac aagctgcagt cgcgccgtgc caacgagcgc agggatcagg
     1441 tctcgcccgg aacgagtcca aactagttgg gctggtcgaa ggatcagcgg caacgctccc
     1501 agcgacaacg cagggagcca cgattgacgc agcatcgcta ccggcagcgc catcagaacc
     1561 gagaccacga gtatcgcgat aaaggccaga cgcgtcctat gctctccgag cctcaccgcc
     1621 aatgtcatct tccctgcatc ggtgtcggtt gggatatcgc gaaggttgtt cgccatcaac
     1681 actgcaactg caagaagccc gacggggatc gatgccatca gagccactgc ggaaacagtg
     1741 aggtcctgga tgaaagtgga accgacggtt gcgaccacac cgaagaatat gaagacgaag
     1801 acctcgccaa ggcccgcaga cgcgtatggt cgtggacctc cgctgtagag aacggccgag
     1861 atgatggcga gcgctccgac aggcagcagg agccacgaca cctgccaggc cagcacagag
     1921 ccgacaatgc ccgcagtcgc gaacgttccc aacatcgccc tacgcatcgc ggctggtgcc
     1981 acgagtccgg aagccactgc tctgcggggc ccagtgcgct cgggtgtatc taccccacgc
     2041 cggccatcga agtagtcgtt ggcaagattc accccgatct ggagagtgac tgcgatgacc
     2101 agagctccga cgactcgcca cgcatgaaac tcatgcgctg ccgaggtgcc gacaaggacc
     2161 ggaacgaccc caacggagag tgtctttggg cgtatggcct ctaaccagac ggacggcatc
     2221 tgcccctcaa ggtcgcttgg ggaatctctc gaactccggg cgcctgcgtt gtacatacgc
     2281 atcccggccc tcctgcgcct cttcactcat gtagtagagg agcgttgcat cgccagcgag
     2341 ttgctggatc cccgcgagac catcggtatc cgcattgaag gaggccttga gaagccgcag
     2401 cgctagtggg ctcatctcca acatccttcg cgcccaagcg actgtctctc gttcgagatc
     2461 ctccagcggc accacggtgt tgaccagccc catgtccagc gcctgctgcg cgtcgtactc
     2521 ccggcagaga taccagatct cgtgcgcctt cttgtggcca acgatcctgg ctagcagccc
     2581 tgcgccatag cccccgtcga atgaaccgac cttaggaccc gtctgtccga agcgagcgtt
     2641 gtgcccggcg atggtgaggt cacagacgat gtgcaggaca tgacctccgc cgatcgcgta
     2701 cccggcaacc atcgcgatga ccggtttcgg gagcctgcgg atctggacct ggagatcgag
     2761 cacattgagc cggccgatgc ccttctcatc gaggtagccg tcgttgcctc ggacccgctg
     2821 atcgccgcct gaacagaacg ccagatcccc ggcccccgtg aagatgatga ctccgacggt
     2881 aggatcatcc ctggcaacat cgaatgcacg agacagttcg aatagagtct tgggtcggaa
     2941 ggcgttccgc acctctgggc ggttgatcgt gatcttcgcg atcccatccc agacctcgta
     3001 gatgatgtct tcgtactcgc ctgatgcctt ccaagcgggt tggtaaggat tttccatagc
     3061 gatagccatc actcctagag cgaaaactcg aagcgttgga ggtaccgtaa caggtcatga
     3121 cgcagggcgc cctcgtagct gttgacacca ccgaccccat cgccttctac cggcagatga
     3181 ccacctgttg ggagaatggc gacgcagtcc tgcccgtcga ccatcggctt tccagagcgg
     3241 gggcacgcgc cctcatcgat agattcaagc ctgcctactt catcgaccag cggcgggtga
     3301 tcgggcttgc aggcggggtc cccgtgagcc ccgggatagc actggtcgta gcgacatcgg
     3361 gaaccaccgg cgaaccgaag ggcgtcgaac tgacgcacgc caaccttgcc gcttcggcat
     3421 acgcaaccgc gcgctggctt gggaccggtc ctgaggatcg atggtgctgc tgccttccac
     3481 tcgcacatat cgcggggatc tcaactttga cgcgttcgat catggccgac gctgaacccg
     3541 agttcacgac tttcggcgtc gatgagatcc tgcggacgaa ggcgacctgc atatccctgg
     3601 tggccacgat gctcgcccga ctgatcgagg ccgaggtgga cctcaccaga ttcagggtca
     3661 ttctcgttgg aggagggccg accccgcgct cgctgatcga gatcgcacag cgctcgggtg
     3721 cccgagtcgt cgcaacgtac ggcatgaccg agacaacggg cggatgcgtc tacgacggca
     3781 acgcccttcc cggggtcgat atcgagatcg gggcacacgg cgtcatcagg ctgcatggcc
     3841 cgatggtgat gtctggttat cggctcgacg agatgggcac ggagcgatcg cttcaggatg
     3901 ggtggttcga aacatcggat ctcggctccc tagactcagg gaagctgaca gtcctcggcc
     3961 gccg
//
LOCUS       DATDOZ010000228         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_977320, whole genome shotgun sequence.
ACCESSION   DATDOZ010000228 DATDOZ010000000
VERSION     DATDOZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977320"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VI541_04615"
     CDS             <1..384
                     /locus_tag="VI541_04615"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLF70219.1"
                     /translation="GPDESTRAALANDFAADLLLSIHLNSHHDPLAAGASSYYFEGSN
                     IASEPGEHLAQLLQEELTAGGRIDCRAHGKSYPILRETSMPAVVLEPGFITDPDEVKV
                     LSDQAGVDDLVTQIMTALRRYFDEP"
     gene            complement(636..821)
                     /locus_tag="VI541_04620"
     CDS             complement(636..821)
                     /locus_tag="VI541_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70220.1"
                     /translation="MAILRRILKVFLMAAGVGLLAGALAPRRQQDPDEVIALDENISE
                     QVAASKRASKLVTARLN"
     gene            complement(822..1694)
                     /locus_tag="VI541_04625"
     CDS             complement(822..1694)
                     /locus_tag="VI541_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLF70221.1"
                     /translation="MNPRGGLGRGLSALIPTGARVMEEIQIDLIVSNPNQPRKRFDPE
                     ALSDLAASIKQLGLLQPVVVRRREEGGYEIVMGERRWRASRNAGLSVIPALVVETDDI
                     GSLERALVENLQRQDLNPLEEAAAFAQLIEDAGFTHEVLAERMGVSRPTVTNALRLLE
                     LPDQIKKMIIDGKLSGAHGRALLGLTGHPLQERTAQRVAAEALSVRQTEEMVRRAREA
                     GSANPPESRIKTQSPGVLDMAERLTDLLGTRVEVSMGGRKGKIVITIGSADELRRIAD
                     LIEGSSRTSREKES"
     gene            complement(1691..2551)
                     /locus_tag="VI541_04630"
     CDS             complement(1691..2551)
                     /locus_tag="VI541_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLF70222.1"
                     /translation="MTENAFPLPESLRVIAIANQKGGVGKSTSAVNLSAWLALEGARV
                     LLVDLDPQSNSTTGVGIDPHRTSGNIYQVLVDKGSIEDAIEPTAVKNLFCVPSTIDLA
                     GAEIELVPVFSRELRLREGLKPVLTEFDYIFIDCPPSLGLLTVNALAAALEVVVPIQC
                     EYYALEGLGQLMRNITLVQSSLNAELHLAGIILTMFDPRTRLAEQVAAEVRNHFPGRV
                     YPDVIPRSVRLSEAPSYGLPIALYDPKSKGALAYRSVARSLAERPPTVETSEDKTEPG
                     ATAETQRGEI"
     gene            complement(2558..>2784)
                     /locus_tag="VI541_04635"
     CDS             complement(2558..>2784)
                     /locus_tag="VI541_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70223.1"
                     /translation="PLCRVGGVVILTAGPGETLGPLAHEGLGTGESSLVRIEGPLDIS
                     QHAFIIPKIKATSKDFPRRPGVARRRPLNI"
BASE COUNT          496 a          881 c          841 g          566 t
ORIGIN      
        1 ggaccggacg agagcacccg tgccgccctg gcgaacgact tcgcagcaga tctgctgctc
       61 tcgatccatc tcaactcaca tcacgatccg ctcgccgccg gggcctcctc ctactacttc
      121 gaggggtcta acatcgcctc agaaccgggc gaacacctgg cccaacttct ccaggaggag
      181 ctcaccgcgg gcgggcgcat cgattgcaga gctcacggga agtcctaccc gatacttcgc
      241 gagaccagta tgcccgcggt ggttctagag ccgggattca taaccgatcc ggacgaggtc
      301 aaggtcctct ctgatcaggc gggtgtcgat gatctcgtga cgcagatcat gacggcgctt
      361 cgcagatact tcgacgaacc ctaggctcct acgccttctt gttcccgatc agatgccgta
      421 gtcgtgagct gcatagcctc cgcccacgaa gctcaacgtc tcgtcgaaag gattccgatg
      481 aaacgtgctc catcttgacc tgcgtggcga tggctatgct gctgacagtg cccgcttcgg
      541 ccaccacggt atcaggcagc gcgcttgtgc ctaccgccga tgtatcgcgc acacgcatct
      601 attcgaccag gagcggggga atccctgcct ctgacctagt taagtcgagc ggttactagt
      661 ttcgaggccc gcttcgacgc cgctacctgc tccgagatgt tctcgtcgag tgcgatgacc
      721 tcgtctgggt cctgctgcct acgtggtgct agggcgcctg cgagcaggcc gactccagcc
      781 gccatcagga agaccttgag gatccttctc agtatggcca tctaggactc cttctctctc
      841 gaggttcgcg atgatccttc gatcaagtct gcgatcctgc gcagctcgtc cgctgagccg
      901 atcgtgatca cgatcttgcc tttcctgccc cccatactca cctcgacacg cgttccgagc
      961 aagtcggtta gacgctcggc catatcgagt acgcctggac tctgcgtttt gatcctggac
     1021 tccggcgggt tcgcagaccc ggcctcgcgc gctctgcgga ccatctcctc ggtctgacgg
     1081 acagagagtg cctccgccgc gactcgctga gcggtgcgtt cctgaagcgg gtgccccgtc
     1141 agaccgagaa gggcgcgacc atgggctccc gagagcttgc cgtcgatgat catcttcttg
     1201 atctggtcgg ggagttcgag aagacgcaga gcgttggtga ccgtgggtcg tgatacgccc
     1261 atccgctctg ccaggacctc gtgcgtgaac cccgcatcct ctatcaactg cgcaaacgca
     1321 gccgcctctt cgagcggatt cagatcctgc ctctggagat tctcgaccag ggccctctcc
     1381 agcgaaccga tatcgtccgt ctcgacgacg agggcgggga tgaccgagag ccccgcgttt
     1441 cgcgacgccc gccatcgcct ctcccccatc acgatctcgt aacctccctc ctcgcggcgc
     1501 cgcacaacaa ccggctgaag aagcccgagt tgcttgatcg atgcggcgag gtcggagagc
     1561 gcctcggggt cgaagcgttt cctgggctgg ttcgggttcg agacgatcag gtcgatctgg
     1621 atctcctcca tgacccgagc ccccgttggg atgagtgcgc tcagaccgcg tccgagtcct
     1681 ccacgtggat tcatatctct cctctctggg tttctgccgt tgcgccgggt tcagtcttat
     1741 cctcggaagt ctccacggtt ggcggccgct cggcgagaga acgagccacg gacctgtagg
     1801 cgagggctcc cttgctcttc gggtcgtata gagcgatggg caggccataa gacggggcct
     1861 ccgagagccg gacactacgc gggatgacgt ccgggtagac ccttccgggg aagtggttcc
     1921 tcacctcagc tgcgacctgc tcggcgagac gggttctcgg gtcgaacatc gtgaggatga
     1981 tcccggcgag gtgcagctcg gcattgaggc tgctctgcac caaggttatg ttgcgcatca
     2041 gttggccgag cccctcgagg gcgtagtact cgcactggat cgggaccacc acctcgagtg
     2101 cggctgccag ggcgttgact gtgagcagcc caagcgaggg ggggcagtcg atgaagatgt
     2161 agtcgaactc agtcaggacc ggcttaagcc cctcacgaag cctcagttct ctggaaaata
     2221 cagggaccaa ctcgatctca gccccagcta ggtcgatggt cgaagggacg cagaagaggt
     2281 tcttgactgc ggtcggctct atggcatcct cgatactccc tttatctacc aacacttggt
     2341 agatattgcc actggttcgg tgggggtcga tcccgactcc tgttgtggag ttcgactggg
     2401 ggtcaaggtc cacaaggagg actcgagcgc cctcgagtgc gagccaggcg gagaggttga
     2461 ccgcgctcgt gctcttaccg acgcctccct tctggttggc gatggcgata acacggagtg
     2521 actctgggag ggggaacgcg ttctcggtca aatcgtctca gatgttcagg gggcgccgcc
     2581 gggcaactcc cggtctgcgt ggaaagtcct tggaggtggc cttgatcttg ggaatgataa
     2641 acgcatgctg agagatatca aggggtccct cgatgcgaac cagactcgac tcgccggtgc
     2701 cgagcccctc atgtgccagg gggcccaggg tttcacccgg cccagcggtg agtatcacca
     2761 ccccgcccac cctacagagc gggg
//
LOCUS       DATDOZ010000229         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2817380, whole genome shotgun sequence.
ACCESSION   DATDOZ010000229 DATDOZ010000000
VERSION     DATDOZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2817380"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VI541_04640"
     CDS             <1..204
                     /locus_tag="VI541_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF70224.1"
                     /translation="EGPGIPTKERERIFQRFYRIENSDSAVGGTGIGLAIVSEFSHAQ
                     GGKAWVEPRPGGGSIFKVALKAG"
     gene            205..879
                     /locus_tag="VI541_04645"
     CDS             205..879
                     /locus_tag="VI541_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70225.1"
                     /translation="MTRSIEQDLDSAEAAVAQAGSLKETGFWRAVAELRSDPALSEKY
                     ASRVARIDRAAFEKAVKLRAGAGLGVAALALATAAGVALVFVSGRSEGLIRDALFLAG
                     FGALDVATHSLAHWVVGRAVGIRFTHVFIGGPPPPRPGLKSDYESYLRTAPRRRALMH
                     ASGAVVTKLIPFALVPVAWQVPTSTWVVWVLLVVGVGQIITDILFSTKTSDWKKFRRE
                     WRAGAK"
     gene            complement(872..1288)
                     /locus_tag="VI541_04650"
     CDS             complement(872..1288)
                     /locus_tag="VI541_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLF70226.1"
                     /translation="MTAELLYLDSSAIVKLISRESESTALATRLAEGPDVVTSALAQV
                     EVLRAMSRVHASRTEVRRAEAVLGAITMLRIDDIVLERAATIRPVTLRSLDAIHLASA
                     VSVGETLTALVTYDARLASAAEGLGVDVLTPSDQIT"
     gene            complement(1285..1542)
                     /locus_tag="VI541_04655"
     CDS             complement(1285..1542)
                     /locus_tag="VI541_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLF70227.1"
                     /translation="MRELRQNLSVYLRKVVKGATLEVTEHGHPVALLMPIPLSNGPIA
                     QLVGTGRARAAEGSLLDLLPPKGRASRRLTRALEDERADRL"
     gene            complement(1603..2406)
                     /locus_tag="VI541_04660"
     CDS             complement(1603..2406)
                     /locus_tag="VI541_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HLF70228.1"
                     /translation="MSERPKEGLVSAAFFDVDKTLIPGSSLFLLARGMYARDFIRVRD
                     IVRFGWGQAAFRLLGREGDRGIEQSKSSTLEFVTGRHQDELRAWGREIVDERVLPRVY
                     EDVSRIIESHKIAGDQTYLVTATPIELADYLAEALGMTGALGTESEIDDQGRYTGQLV
                     GDILHGESKSKAVAQLASERGIDLDSSSAYSDSHNDLPLLGSVGHPHAVNPDATLRRI
                     ARTRGWPIHELRTRRRALLIGIPSALGGAAVFGLGAATGAAIMKRKRNS"
     gene            complement(2399..>2815)
                     /locus_tag="VI541_04665"
     CDS             complement(2399..>2815)
                     /locus_tag="VI541_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HLF70229.1"
                     /translation="EGFVVLPYTNDDPVLAKRLEDAGCAAIMPLGSPIGSGSGIRNPY
                     NISIIVEQAGVPVILDAGIGTPSEAAQAMELGCDGVLIASSISRAKDPVGMATAMRLA
                     VEAGHLGARSGRIPRRLYAQGSTPMEGLAELGGEDE"
BASE COUNT          501 a          884 c          896 g          534 t
ORIGIN      
        1 gaaggtcccg ggatccccac gaaggagaga gagaggatct ttcagcgctt ctatcggatc
       61 gagaactccg atagtgccgt cggtggaaca ggcatcggcc tcgcgatcgt gtccgagttc
      121 agccatgcac aggggggcaa ggcctgggtt gagcctcgcc ccggcggcgg gagcatcttc
      181 aaggttgctc tgaaggctgg ctgagtgacg cgctctatcg agcaggacct cgactccgcc
      241 gaggccgccg ttgcccaagc aggatcactc aaggagactg gtttctggcg cgccgtggcc
      301 gaacttagat ctgacccggc actgtccgag aagtacgcct cgcgagtggc ccgtatcgac
      361 agggccgcat tcgagaaagc cgtaaagctt cgtgctggcg cgggcctcgg cgttgcggcg
      421 ctagcactcg ccaccgccgc gggggtggct ctcgtgtttg tgagcggccg atccgaggga
      481 ctcatccgtg acgccctctt cctggccggg ttcggggccc tcgatgtcgc gacccacagt
      541 ctcgctcact gggtcgttgg cagggcagtg gggatccggt tcacgcatgt attcatcggg
      601 gggcccccgc cgccgcggcc tggcctcaag agcgactacg agagttatct tcgcaccgcg
      661 cctcgaagac gagccctcat gcatgcatcg ggggccgtcg tcacgaaact catcccgttc
      721 gcactggttc cggtcgcgtg gcaggtgccg acctcgacct gggtggtctg ggtactactc
      781 gttgtcggtg tcggccagat catcaccgac atcctcttct cgaccaagac ctcggactgg
      841 aagaagttcc gccgcgagtg gagggcgggt gctaagtgat ctggtctgag ggcgtcagca
      901 cgtcaactcc gagaccttct gcggccgacg caagccgtgc atcgtaagta acgagcgctg
      961 tcaaggtttc acccaccgac actgccgaag caaggtgtat cgcatctaag gagcgaagcg
     1021 tcaccggacg gatggtcgcc gctcgttcca agacgatgtc atcgatacga agcatggtga
     1081 tcgcgccgag cacagcttca gcccggcgca cctcggttct ggaggcgtgg actcgactca
     1141 tggctcggag tacctcgact tgcgcgagcg cgctcgtcac gacatcaggg ccttcagcca
     1201 gtcgcgtagc gagtgccgtg ctttccgact cgcgcgagat cagcttgacg atggcggagg
     1261 aatcgaggta caggagttca gcggtcacag acggtcggct cgctcatctt cgagggcgcg
     1321 cgtcagccgc cgacttgctc gccctttggg gggaagaaga tcgagcagcg acccctcggc
     1381 ggcccgagct cgcccagttc cgacgagttg agcgataggt ccgttcgaaa gagggatagg
     1441 catgagcaga gcaaccggat gtccgtgctc ggtgacttca agggtcgctc ccttgactac
     1501 cttgcgaagg tagacgctga ggttctgtcg gagctcgcgc accccaactc tttcacctga
     1561 tgcccccatg tagcacatgg tagcataatc gagggttgag ctctagctgt tgcgtttgcg
     1621 cttcatgatc gccgcgccgg ttgcggcccc cagcccgaac acggctgcgc caccgagggc
     1681 tgaaggtatc ccgatgagaa gggcgcgtcg gcgggttctc agttcatgga tgggccaccc
     1741 gcgtgtgcgg gcgatcctac gaagggttgc atcagggttg acggcgtggg ggtgccccac
     1801 ggatccgaga agcggcaggt cgttgtgtga gtcggagtag gccgacgaag agtcgaggtc
     1861 gatcccgcgc tctgacgcca actgtgcgac ggccttgctc ttggattccc catgcaggat
     1921 gtctcccacc agctggcctg tgtacctgcc ctggtcatcg atctctgact cggtccccag
     1981 ggcgccggtc atgccaaggg cctcggcgag gtagtccgca agctcgatcg gtgtggccgt
     2041 gacgaggtag gtctggtcac ccgcgatctt gtgagattcg atgatgcgcg aaacatcctc
     2101 gtagacgcgg ggaaggaccc tctcatccac gatctcccgg ccccatgcgc gcaactcgtc
     2161 ctggtgtcgt cctgtgacga attcgagggt cgatgacttc gactgctcga tgccccggtc
     2221 cccctctcgc cccaacaaac ggaaggctgc ctgcccccaa ccgaagcgga cgatgtcgcg
     2281 cactctgatg aagtcgcgcg cgtacatccc gcgcgcaagg agaaagagac tcgacccagg
     2341 tatcagggtc ttgtcgacat cgaagaaggc ggcgctgacc aggccctcct tgggtcgttc
     2401 actcatcttc acctcccagt tctgcgagtc cttccatcgg cgtagagccc tgcgcgtaga
     2461 gcctgcgcgg tatgcgaccc gaacgggctc ccagatgacc cgcttcgaca gcgagtcgca
     2521 tcgctgtggc catcccaacc ggatctttcg cccgcgatat cgaactggct atcaacacac
     2581 cgtcacagcc gagctccatc gcctgagcgg cctccgaggg cgtgccgatc cccgcatcga
     2641 ggatcaccgg cacacccgcc tgctcgacga tgatcgagat gttgtatggg ttgcgtatcc
     2701 ccgatccact gccgatgggg gagcccaggg gcatgatggc agcgcatcct gcatcttcga
     2761 gccgctttgc tagcacaggg tcgtcgttcg tgtagggaag cacgacgaaa ccctc
//
LOCUS       DATDOZ010000230         2810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2817416, whole genome shotgun sequence.
ACCESSION   DATDOZ010000230 DATDOZ010000000
VERSION     DATDOZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2817416"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VI541_04670"
     CDS             <1..437
                     /locus_tag="VI541_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HLF70230.1"
                     /translation="VLFAIGWFLFTYRGYERKDDIAGNLACVFALGVALFPNSGEPSE
                     RAIHFTSAGLLFLVLAYFSLVLFTKSGGTPTAQKLKRNRVYVVCGVSILLCIAGIGLY
                     YAFLQNSSIAALKPVFWLESLALWTFGVSWFVKGETLWRDPD"
     gene            643..2358
                     /locus_tag="VI541_04675"
     CDS             643..2358
                     /locus_tag="VI541_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70231.1"
                     /translation="MRIRSMLSLVSVLALIAVLPAGADTGDVGNPAERYVARPAGVEK
                     DMTFFFGPYNIPPGQDLNRITLDVPLSTGFYTAIAPNLVDAVTGEEPAHQVAHIHHAH
                     WLQIVGDSDHERYPLYGPTDPYVGLSWVFGTGEEKTQGSLNDRADADPLGPRYGMFID
                     GSQPQSLIFMVHNKTSGPLNVYILLEVRFAFGTRDEIKNATGCLLLLDGESCVAGKDF
                     HSLTGKLWGTTFDVPRGVGANGTYVHPRDIPPSLGTASPLGRFFTAGWNGTAIAGAGH
                     LHPLGKEVVLVNLGSVAKPCADLDNDGYAGITIFHSRKMERVPEANFTTEDYQMGATK
                     PGFRTPIRQGDRIAQFGVYRNDENATYEAMSYSGLYVDKLQVPTPLAANEGCTLANHG
                     SYLINPSGPADLPTEGMLNHAWHGSPDTLCEVPGTIFATVDCNRDENFSVEWPKRQLT
                     DTVHIANFLYSSGDRSVSGPLRVLPQVKQGSSIRFINEDAALVIRHSVTSCPFPCNGP
                     YVANYPLPDGVFESHVLGNIDPINGAPDNTKFDWKTPTDLSVGVYSYFCRIHPTMRGV
                     FEVIA"
     gene            2421..>2810
                     /locus_tag="VI541_04680"
     CDS             2421..>2810
                     /locus_tag="VI541_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70232.1"
                     /translation="MRSRIVSLFVVLALGAMGALRANEGPAETLPPSVPVGFAIAPQA
                     TVPGAASISISPNGSKLYVASYAGLVLTYPLTAGVVTGSPTTFLSGLSSPLGVLATDI
                     GAYVATNGPTSVTYNSISYVEGIISFAR"
BASE COUNT          531 a          843 c          834 g          602 t
ORIGIN      
        1 gggtcctctt cgcgatcgga tggttcctct tcacctatcg gggatacgaa cgcaaggacg
       61 acatcgctgg caacctcgcg tgcgtcttcg cgcttggcgt tgcgctcttc ccgaatagcg
      121 gtgagccttc ggagagggcg atccatttca catcagcagg tcttctcttc ctggtcttgg
      181 cctacttctc gctggtcctg ttcactaagt caggagggac ccccacagcg cagaagctga
      241 aacgcaatcg cgtctatgtc gtctgcggtg tgtcgatact cctgtgcatc gcggggatag
      301 gtctctacta cgcgttcctg cagaactcat ctatcgcagc gttgaagccg gtcttctggc
      361 tcgagtcgct ggctctctgg accttcggcg tctcgtggtt cgtgaaaggc gagacgctgt
      421 ggagagaccc cgattagtac ggccagagcg agtagatcac cgccgggcca cagaagctga
      481 cgagcgcgcc ggggaggatg gcctcgctca ggcaggaatc gggcctgggg cctcgaatca
      541 ttggaacagc agttgcctac cgctggataa gttgattgga tgggtcagtt gtttcctgct
      601 gtgggagagc cccaggcggg gcttagggag aggggctttc aaatgcgcat ccgttcgatg
      661 ctgtcgctcg tgtcggtatt ggctctgatc gcagtgctgc cggcgggggc cgacactggc
      721 gatgttggca accctgctga gcgatacgtc gcaagacctg ctggcgtcga gaaggacatg
      781 accttcttct tcggccccta caacatcccg ccggggcagg acctgaaccg gatcacgctc
      841 gacgttcctc tctcgacagg tttctacacg gcgatcgcac cgaacctcgt cgatgcggtg
      901 acgggcgagg agcctgctca ccaagttgct cacatccacc atgctcactg gctgcagatc
      961 gtgggtgatt cagatcatga gcgatacccc ctctacggac cgaccgatcc ctatgtcggg
     1021 ctctcttggg tcttcggcac cggagaggag aagacgcagg gaagtctcaa cgaccgtgcc
     1081 gacgccgatc cactcggccc ccgctatggg atgttcatcg atggctcgca gccacagagc
     1141 ctgatcttca tggtccacaa caagacgagt ggtccgctca acgtctacat cttgctagag
     1201 gtgcgcttcg cgttcggtac gcgcgacgag atcaagaacg cgaccggttg cctacttctc
     1261 ctcgacggcg agtcctgcgt ggcgggcaag gacttccact cattgaccgg caagttgtgg
     1321 gggacgacat tcgacgtgcc gcgaggtgtg ggggccaatg gtacttatgt tcatccccgc
     1381 gacatcccac ccagtctcgg gaccgcaagt ccgctgggta gattcttcac cgctggatgg
     1441 aacgggaccg cgatcgcggg ggccggacac cttcatcctc tcggcaaaga ggttgtgctg
     1501 gtcaacctag gatctgtcgc taagccatgc gctgacttgg ataacgacgg ttacgctggg
     1561 atcaccatct ttcactcgag aaagatggag cgcgtgccgg aggccaactt cacgactgag
     1621 gattaccaga tgggcgcgac gaagcccggc ttccgtacac ccataaggca gggggatcgc
     1681 attgcccagt tcggtgtcta ccgcaatgac gagaacgcga cctatgaggc gatgagttac
     1741 tcgggtctat acgtcgacaa gttgcaggtc cctacaccgt tggctgcgaa tgagggctgc
     1801 accctcgcta accacggttc atatctgatc aatccttctg ggcccgcaga ccttccgacg
     1861 gaggggatgc tcaaccatgc atggcatggt tcgcccgata ctctctgtga ggtccctggg
     1921 accatcttcg cgacagtcga ctgcaaccgt gacgagaact tctcagtgga gtggccgaag
     1981 cgccagctga cggataccgt gcatatcgca aacttcctgt actcatcagg tgaccgttcg
     2041 gtctccgggc cgttgcgcgt tctcccgcaa gtcaagcagg ggagcagtat caggttcatc
     2101 aatgaggatg ccgcgctggt gatccggcac agcgtcacgt catgtccgtt cccatgcaac
     2161 ggaccttacg tcgccaacta tcctctcccc gatggtgtct tcgagtcgca tgttctcggc
     2221 aacatcgacc cgatcaacgg ggcccccgac aacaccaagt tcgactggaa aacgccgaca
     2281 gatctgtcgg tcggggtcta ctcctacttc tgccgtatcc acccgaccat gagaggggta
     2341 ttcgaggtca tcgcctgatt tcgaccggat ctttccggcc agcaggaaat cgggagcggg
     2401 cctagaactc gatagagatg atgagaagcc ggatcgtgtc gctcttcgtc gttctcgcgc
     2461 tcggtgcgat gggggccctg cgggctaatg aggggccagc ggagacgcta ccgcccagcg
     2521 ttccggttgg gttcgcgatc gcgccccaag cgaccgtccc tggggcagcc tcgatatcga
     2581 tatcgcccaa tggctcgaag ttgtatgtcg cgtcatatgc gggcctagtc ctgacatatc
     2641 cgttgacggc gggcgtcgtg accggttcgc cgactacgtt tctttcggga ctctcatccc
     2701 cactcggtgt tctcgccact gacatcggcg catatgtcgc gacgaacggg ccgacttcag
     2761 tgacctacaa ctcgatcagc tacgttgaag gcatcatctc tttcgctcgc
//
LOCUS       DATDOZ010000231         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3356092, whole genome shotgun sequence.
ACCESSION   DATDOZ010000231 DATDOZ010000000
VERSION     DATDOZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356092"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1162
                     /locus_tag="VI541_04685"
     CDS             50..1162
                     /locus_tag="VI541_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLF70233.1"
                     /translation="MLTRFPRFTIWVTTAVAVAVVGNAALPAFGAGEAAQLDQTRQRI
                     NEIRRNLAAAKGRASVVEAQVNALQDQIDALSREIRSDEKNVSSLESDIRTAEAKLTD
                     LEARYSRAAQASNDRARRLYTSTPIDAISHMLSAKNVTEFTRQQVFLEISSQADGKAM
                     IESTRIKALLLEQKTYLVGVRDNLAARRQWLADRRNLIASARNDKASALSSIEAEIRE
                     ELAEIRALEAQSRALTAALRGSVSRSSGAVSRAGFIWPLRGAITSPYGKRWGGFHPGI
                     DIDGNTGDPIRASKSGDIVGVACGSGYGLCTIIDHGGGVATLYAHQSRKAIGGGHVNQ
                     GDVIGYVGCSGSCTGSHLHFEVRVDGSPRNPRDFLP"
     gene            complement(1228..1518)
                     /gene="groES"
                     /locus_tag="VI541_04690"
     CDS             complement(1228..1518)
                     /gene="groES"
                     /locus_tag="VI541_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HLF70234.1"
                     /translation="MSLKPLGDRVLVRAEESEDQLPSGLVIPDTAKEKPQEGIVLAVG
                     PGAYFEGKRVPLDVKEGDRVVYSKYGGTEIRVGREDLLILSERDILAIVTKK"
     gene            complement(1632..2585)
                     /locus_tag="VI541_04695"
     CDS             complement(1632..2585)
                     /locus_tag="VI541_04695"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HLF70235.1"
                     /translation="MRRDREVDAIAASQHGLINRGQAAAAGLSDRCVNGRLKSGCWVR
                     VLPSVYRVAAAPESQDQLLIAAWLWSKGRAVVSHRSAAFFLRLDGFDAIPVDISIAAS
                     SRSPDSLIVLHVQRMPRLRNIVKTGMFTLTDATRTLIDLGSVVDEPTVEAALEDALRR
                     RLTTTKKIEAMLRDIGGPGRRGSGVLRAIMEARGPQAAAAESILEVRLLRLMKASGLP
                     APIRQHEVRVKGRVVARLDLAYPQSRLAIECDGFRWHGGREAWQRDVHRSNALVGLGW
                     RVIHVTWSDIEVRSGDVIADIRLALEGGSEGLFAIRYLPGT"
BASE COUNT          561 a          890 c          806 g          474 t
ORIGIN      
        1 aagacggcat gtttgacaca tcaaaaccat ccggctaacc tcccccgaga tgctgaccag
       61 attcccaaga tttaccatat gggttactac tgcggtcgcg gtcgcagtgg tcggaaacgc
      121 cgccctcccc gccttcggcg ccggtgaggc tgcgcagctt gaccaaacgc gccaacggat
      181 caacgagatc cggcgcaacc tcgctgccgc caagggccga gcgtcagttg tcgaggcaca
      241 ggtcaacgca ttacaggatc agatcgacgc actcagccgc gagatccgca gtgacgagaa
      301 gaacgtctct agcctagagt ccgacatcag gaccgccgag gccaagctga ccgacctcga
      361 ggctcggtac tcaagggcgg cgcaggcatc caacgaccgc gcccgccgcc tctacacctc
      421 tacacccatc gacgccatct cccacatgct ctcggcgaag aatgtgactg agttcacccg
      481 tcagcaggtc ttcttggaga tctcctcgca ggccgatggc aaggcgatga tcgagtcgac
      541 caggatcaag gccctgctct tggagcagaa gacctacctg gtgggggtcc gagacaacct
      601 ggccgccaga aggcagtggc tcgcggaccg ccgcaacctc atcgcttcgg ctcgcaacga
      661 caaagcttcg gccctctcct cgatcgaggc ggagatccgt gaggagctgg ccgagatcag
      721 ggctttggag gctcagagcc gggcgctcac agcagcactg cgtgggtcgg tctcacgttc
      781 gagcggcgcc gtctcgcgag ccggcttcat atggcctctg cgcggagcga tcacatcacc
      841 gtacggaaag cgctgggggg ggttccaccc cggcatcgac atcgacggaa atacagggga
      901 ccccatccgc gcctcgaagt ccggagacat cgtgggggtc gcttgtggtt caggctatgg
      961 gctatgcacg atcatcgacc atggcggtgg ggtcgcgacc ctttatgctc accagagccg
     1021 caaggccatc ggcggagggc acgtgaatca gggcgacgtc atcggttacg tcggttgctc
     1081 gggctcgtgc acggggtcgc atcttcactt cgaagtccga gtcgacggat cacctcgaaa
     1141 ccctcgggac ttcctgccct aaacccggac atgacatccg catcaaaaaa gccccgccga
     1201 ggcggggcct ttttgatcct agaggagtta cttcttggta acgatcgcga ggatgtcacg
     1261 ctcgctgagg atcaatagat cctcacgacc gactcgaatc tcagtgcccc catacttcga
     1321 gtagacgacg cggtcgccct ctttgacgtc gagcggtact cgcttgccct cgaaataggc
     1381 gccggggcca actgccagga cgatgccctc ctggggcttc tccttggcgg tgtcggggat
     1441 aaccaatccc gagggaagct ggtcctcgga ctcttcagcc cttaccagta ccctgtcacc
     1501 caaaggcttg agactcatct atgacctcct gtcgaaaaac tctcgttagc actcccagat
     1561 ggggagtgct aagacaataa ctgagggatc caagggccgt caaccgcccg ctcacacctg
     1621 tcctttcgcg attaggtacc cggcaggtac ctaatcgcga aaagaccctc cgaaccacct
     1681 tccagcgcga gtcggatatc ggcgataacg tcgcctgagc gcacctcgat gtcgctccag
     1741 gtgacgtgga tcacacgcca gcccaggccg acgagagcgt tgctgcgatg cacatctcgt
     1801 tgccaggcct cacggccccc atgccaacgg aagccgtcgc actcgatggc aagcctggac
     1861 tgcgggtagg ccaggtcgag gcgtgccacc accctgccct tgaccctgac ctcgtgctgg
     1921 cggatggggg ccgggaggcc cgacgccttc atcagccgca ggagacggac ctccaggatg
     1981 ctctctgcgg ccgccgcttg ggggccccga gcctccatga tcgcccgcag cactccggat
     2041 ccccgccgcc ccggcccccc gatatcgcga agcatcgcct cgatcttctt cgtagtggtc
     2101 aaccggcggc gaagagcatc ctccagagcg gcctcaactg tgggttcatc aaccacagaa
     2161 ccaagatcga tcagggtacg agttgcatcc gtcagcgtga acattccggt cttaactatg
     2221 ttgcggagcc gaggcatccg ttgaacatgc aagacgatca gcgagtcggg tgatctgctc
     2281 gacgcagcga ttgagatgtc gacggggatc gcgtcgaacc catcaagccg gagaaagaag
     2341 gcagccgatc gatgtgagac aactgctcgc cccttcgacc acaaccatgc ggcgatcaag
     2401 agttggtcct gggattccgg tgctgcagcc actcggtaaa cgctgggaag aacccgcacc
     2461 cagcatcctg acttcagtcg accattgaca catcgatcgc tcagccccgc cgcagctgcc
     2521 tgcccacggt tgatcaaacc atgctgactg gcggcgatcg cgtcgacctc tcgatccctt
     2581 cgcatgggtc acaagataga cacgaggtat gacaaccgag atccaccgcg gctcctttcg
     2641 cgaataggta cctgtgaggt agtcagtcgc gaaaagaccg ccgcagacga cgtcccgggg
     2701 gcgcgggggc cgggggcgcg ggggccgggg g
//
LOCUS       DATDOZ010000232         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3060318, whole genome shotgun sequence.
ACCESSION   DATDOZ010000232 DATDOZ010000000
VERSION     DATDOZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060318"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VI541_04700"
     CDS             <1..1455
                     /locus_tag="VI541_04700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLF70236.1"
                     /translation="SLQIDYAAIAPELILTAVLCGVLLLDLVLPKERKWLAMPAAFLG
                     VLVTLGAVLWLATGPDRTTLGGMFVVDRFAVLFKVLFSLAALIVFAISHDYLKDDDIH
                     QGEYYTLMLSSLLGMLVIASSRDLIPIFVALELISLPAFVLAGLRKSDIRSNEAALKF
                     FLFGVLSTAVMLFGMSLIYGITGTTDLAKISVVLAATPVFRSIALLSIFFIIVGFGFK
                     ISAFPFQWWVPDTYEGSPVPVAAFLSVASKTAGFVGLLQVMFIAFGGLADVWRPFLAL
                     LAVLTMTFGNLVAMQQKHLVRLLAYSSIGQAGYILLPLGIASATDRSLNHQAMMASIT
                     YLLIYAFMETGAFAAVIAVGRRGGGYMLDNYAGLFTRSPGLAIGMTVFLFSLAGVPPF
                     AGWAAKLFVFLAVIKSGYIWLAIAMALNTVIALFYYALVVKRMFIDKATQGEPIIKVP
                     PLISTAIAVSAIVVLLIGVLPDLFAKIAAASSLL"
     gene            complement(1465..2421)
                     /locus_tag="VI541_04705"
     CDS             complement(1465..2421)
                     /locus_tag="VI541_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HLF70237.1"
                     /translation="MKAVVLAAGAGERQGRLSEERLKPLLPTLDITQLEWNLSSLSLA
                     GASTIYVNTNRHAEQIVRVADRTHESLNIDLRMSHEPGEPLGTAGALRNLRDELDSTF
                     IVVNGDVLCDEPIDKLVTAHASAGAACTLFVVADRHRGDFVIENDWVTGLVDRRESRE
                     PGWVYTGIGIFEPSLIELIPEGPIGLYESVLTAVLQEGRGMACVELAGYWRDVGTPEA
                     HLLSNLEALSGAFSLRSIPGLLGTEASRNDPLAYVGQGAEISGCDLRHCVVGSGARVG
                     AGSRLHRCVVWDNTDVATGDYSEVIITPRGITPARLYEETTP"
     gene            2561..3685
                     /locus_tag="VI541_04710"
     CDS             2561..3685
                     /locus_tag="VI541_04710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLF70238.1"
                     /translation="MSLVFLGAPVALAQIPDLPTEIIGSPSPSPSPAGSSSPTPGPNP
                     NTQPTPSPSATAPAQQTVGPTPFIQIPDIVRTPSQTTTKLLAILAPLEDRGMPLDQAV
                     VLAAAPFPVAGQALFSDDFGFPRSIPVAHAHEGNDIFADFGTPIVASSPGAIAGMADT
                     LVGGLSVWIAADDGTGYYYAHLLSFAEVALGGRVDAGTVIGYVGNTGDAIGTSPHLHF
                     EVHPPILGKRGKVLASGVTSFLDGTARTNTPAVDPKPYLDAWLTQAELRAQGLVIEVV
                     RRFAAVARELYLGNSVEKLSGFDDAPDAVEMVWPSIFEPTLSAMGMADDLARAALSRG
                     AIFDAEAARIQAVRLAMWEPQLRLSAFTGMPLLDYPLAAA"
     gene            complement(3687..4103)
                     /locus_tag="VI541_04715"
     CDS             complement(3687..4103)
                     /locus_tag="VI541_04715"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HLF70239.1"
                     /translation="MDPDCIFCKIIAGEAPATRLYEDDATVCFLDIFPWARGHSLVVS
                     KEHAETIYDLSESAAAATMATVKIVAPILRDAVGAEGLNLLQANGRAAWQSVDHFHIH
                     LVPRWFGDPLRPPGAPSPADPDALKEIAASFVERLS"
     gene            complement(4112..4600)
                     /locus_tag="VI541_04720"
     CDS             complement(4112..4600)
                     /locus_tag="VI541_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HLF70240.1"
                     /translation="MAQFSFDIISEIDKAELANALDQARKEIGQRFDFKNTQTEIKHE
                     EETLEIRANSEGRVEAALDVLKEKMVRRDISLKALDVGKIEPGASSSYKLGIKLVHGI
                     SDEKGRAINKFIKASGTKVQSQIQGGQLRVTSKTKDDLQNVIKLLRDEDFGIPLQFTN
                     YR"
     gene            4675..4755
                     /locus_tag="VI541_04725"
     tRNA            4675..4755
                     /locus_tag="VI541_04725"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4709..4711,aa:Tyr,seq:gta)
BASE COUNT          852 a         1520 c         1405 g         1116 t
ORIGIN      
        1 agcttgcaga tcgactacgc ggccatcgcg ccggaactga tcctcaccgc agttctatgc
       61 ggcgtgctgc tgctcgacct cgttctaccg aaggagcgaa agtggctggc gatgcctgca
      121 gcgttccttg gtgttctcgt cactcttggc gctgtcctct ggctggcgac gggacccgac
      181 cggacgaccc ttggcgggat gttcgtggtc gatcgtttcg ccgtgctctt caaggtcctt
      241 ttcagcctcg ctgccttgat cgttttcgcc atctctcacg attacttgaa ggacgacgac
      301 atccatcagg gcgagtacta cacgttgatg ctctcgtctt tgctcgggat gttggtgatc
      361 gcgtcgagcc gcgacctcat cccgatcttc gtcgcgctgg agttgatctc gctgcctgcc
      421 ttcgtccttg ctggtctgcg caagtcagac atcaggtcta acgaggctgc cctcaagttc
      481 ttcctcttcg gggtcctctc gacggccgta atgctcttcg ggatgtcact catctacggc
      541 atcacaggca ctacggactt ggcgaagatc tcggtggtgc tagctgctac cccagtgttt
      601 cgctcgatag cactcttgag tatcttcttc atcatcgtcg ggttcgggtt caagatctcg
      661 gccttcccgt tccagtggtg ggtgcccgac acctatgaag gctcacctgt ccccgtggca
      721 gccttcctat cggtggcatc caagaccgct ggcttcgtcg ggctactgca agtgatgttc
      781 atcgcgttcg ggggcctggc cgatgtctgg cgtcctttcc tggctctcct ggcggtgttg
      841 accatgacct tcggcaacct agtcgcgatg cagcagaagc atctggtcag actgctggct
      901 tactcatcga tcggacaggc cggatacatc cttctgccgc tcggcatcgc gagcgcgacc
      961 gatagatctc tcaaccacca ggccatgatg gcgtcgatca cctatctgct gatctacgcg
     1021 ttcatggaaa cgggggcctt cgcagctgtg atcgcggttg gccgtcgcgg tggtggctac
     1081 atgctcgata actatgcagg gttgttcacg aggtcccccg gcctggcgat cggcatgacc
     1141 gtgttcctct tctcgctcgc tggcgttccg ccgttcgcag ggtgggctgc caaattgttc
     1201 gttttcctcg cagtgatcaa gtcgggatat atctggttgg ccatcgccat ggcgctcaac
     1261 accgtgatcg cgctcttcta ctacgcgctc gtcgtcaaga ggatgttcat cgacaaagct
     1321 acccaaggcg agccgatcat caaggtgcct cctttgatct cgacggctat cgctgtctcg
     1381 gcgatagttg ttctgttgat cggcgttctc ccggaccttt tcgccaagat cgcggccgcc
     1441 tcgtcgctcc tctagcacca acagctaagg agtcgtctcc tcgtagagcc gagcaggtgt
     1501 gatcccgcgt ggcgtgatga tcacctcgga gtagtcaccg gtcgcgacat cggtgttgtc
     1561 ccagactacg caacggtgaa ggcgcgatcc tgcccccact cgcgcccccg agccgacgac
     1621 gcagtggcgc aggtcgcacc ccgaaatctc ggccccctga ccaacgtacg cgagggggtc
     1681 gttgcgcgat gcttccgtgc cgagcagccc ggggatgctg cggaggctga atgcgcccga
     1741 gagcgcttcg aggttggaga gtagatgggc ctcgggggtg ccgacatcgc gccagtaccc
     1801 tgctagctcg acacaggcca tcccgcggcc ctcttgcagg accgcggtca agaccgattc
     1861 gtagagtccg atcggacctt cggggatcaa ctcgatgaga ctgggttcga atatccctat
     1921 gcccgtgtat acccatccgg gctctcgtga ctctcgtcga tctaccaatc cggtgaccca
     1981 atcgttctcg atcacgaagt cgccgcggtg ccggtcggcg acgacgaaca gagtacatgc
     2041 cgcaccggcc gacgcgtgag ccgtgactaa cttatcgata ggctcatcgc agagaacgtc
     2101 gccattgaca actatgaaag tcgagtccaa ctcatcgcgc agattgcgaa gagcaccggc
     2161 ggtcccaagc ggttcccctg gctcatgcga catgcgaagg tcgatgttga gactttcatg
     2221 agttcggtcg gccactcgca ctatctgctc ggcatggcga ttcgtattga cgtagatcgt
     2281 cgaggcaccc gcgagagaaa gcgatgacag gttccactcg agttgcgtga tgtcgagcgt
     2341 aggcaggagc ggtttgagtc tttcttccga caatcttccc tgacgttcac cagcccctgc
     2401 ggcgaggacg acagccttca tgtgatgccc atcaacgcat catgcgcgat cgcgacgtga
     2461 cacggtacga tagatgagca gtcagggtcc ttagaatact tctctggagt tctctttgaa
     2521 gccacggaca tcgatactat ttcgtcacgc ggtcgcagcc atgagtctcg tgtttctcgg
     2581 ggcccccgtg gcgttggcgc agatccctga ccttccgacc gagatcatcg gtagtccgtc
     2641 tccgtcgcct tctcctgcgg gctcgtcatc gccgacccct ggtcctaacc ccaacaccca
     2701 gccaacacca tccccttcag ccacggctcc cgcgcagcag acagttggcc caacaccatt
     2761 catccagatc cccgacatcg tccgcacgcc gtctcagacg acaacgaaac tgctggccat
     2821 cctcgctcct ctcgaagatc gagggatgcc cttggatcag gctgttgttc tggcggccgc
     2881 tccatttccg gtcgccggtc aagctctgtt cagtgatgac ttcggattcc cccgaagcat
     2941 ccccgtcgcg catgcccatg agggcaacga catcttcgcc gacttcggca cccccatcgt
     3001 agcgtcgtcc cccggagcca tcgcagggat ggccgacaca ctcgttggcg gtctctcggt
     3061 ctggatcgcc gccgacgatg gaacaggcta ctactacgcg cacctgttga gtttcgctga
     3121 agtcgcattg gggggccgcg tcgacgcggg aacggtgatc ggttatgtag gcaacaccgg
     3181 tgatgcgatc ggtacttcgc cccatctgca cttcgaggtt catcctccga tcctgggcaa
     3241 gcgcggcaag gttctcgcga gcggggtcac ctcgttcctc gacggaaccg ctcgcaccaa
     3301 cactcccgcg gtcgacccga agccctacct cgatgcttgg ttaactcagg ccgagttgag
     3361 agcacaaggt ctagtgatcg aggtcgttcg cagattcgca gcggttgcgc gggaactgta
     3421 tctaggtaac tccgttgaga agctcagcgg cttcgatgat gctcccgatg cggtcgagat
     3481 ggtgtggcct tctatcttcg agccaactct ctccgccatg gggatggccg atgatctcgc
     3541 tcgcgctgcg ctgagtcgcg gagccatctt cgatgcagag gcggcgcgca tccaggcagt
     3601 tcgccttgcg atgtgggagc cgcagctcag gctctcagcc ttcaccggca tgccgcttct
     3661 ggactaccca ctggctgcgg cttaagtcaa ctcagccttt caacgaagct cgcagcgatc
     3721 tctttcagcg catcagggtc ggcgggggat ggggcccccg gtggtctaag cggatcgccg
     3781 aaccatctcg gaaccaggtg gatgtggaag tggtcgacgc tctgccacgc cgctcgtccg
     3841 ttcgcctgaa ggagattgag tccctccgcc cccaccgcgt cgcgcagtat cggagcaact
     3901 atcttcacgg tcgccatcgt cgcagcggct gccgattctg ataggtcata gatcgtctcg
     3961 gcatgttcct tgctaacaac gagactgtgg ccccgtgccc aggggaagat gtcgaggaag
     4021 cagacggttg cgtcgtcttc gtacagacgc gtggcggggg cctcaccagc gatgatcttg
     4081 cagaagatgc agtcggggtc catgcgatct cctaccggta gttggtgaac tgaagcggta
     4141 tcccgaagtc ctcgtctcgc aggagcttga tcacgttctg aaggtcatcc ttcgtctttg
     4201 acgtcaccct cagttggccg ccctgtatct gactctggac cttggtcccc gaagctttga
     4261 tgaacttgtt tatggcacgg cccttctcgt cgctgatgcc gtggacgagc ttgatgccca
     4321 gcttgtagga gctactggcg cctggttcga tcttgccgac gtcaagggcc ttgagcgaga
     4381 tgtcgcgccg taccatcttc tccttgagta catcgagggc tgcttcgacc cggccctcgg
     4441 agttcgcacg gatctcgagc gtttcctctt cgtgcttgat ctcagtctgt gtgttcttga
     4501 agtcgaaccg ctgcccgatc tccttacggg cctggtcaag agcgttcgcc aactcggctt
     4561 tgtcgatctc gctgatgatg tcgaatgaga attgagccac gattaccccc taagagttgg
     4621 atgcatctgt tcgtgggcct tatcctagcg aggctccgct ggtcggggca taacggtggg
     4681 gtacctaagt ggccaaaggg agccggctgt aaaccggttg cgcaagcttc ggaggttcga
     4741 atccttcccc caccacaaaa agtaagggaa agtgcgctat tcgaactcgt gctagagagc
     4801 ctgttcccag gttccgggct ttgggacgac gaaggctatc gattgcgcgt taccgttctc
     4861 ctcgcgacta gcaatctccg caacgaccgc ttt
//
LOCUS       DATDOZ010000233         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3582551, whole genome shotgun sequence.
ACCESSION   DATDOZ010000233 DATDOZ010000000
VERSION     DATDOZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3582551"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VI541_04730"
     CDS             complement(<1..931)
                     /locus_tag="VI541_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF70241.1"
                     /translation="MDLDLPVEVEELRKTVRAFAEEVVRPQAEGFDERAEFPLEIVKE
                     MGKMGLFGLPFPEEYGGMGGDFLALCVAIEELARVDSSVAITLEAGVGLGAMPFFLFG
                     TEEQKMQWLLPMARGEILGGFGLTEPGGGSDAGSTQTTARIDGDDWVINGAKAFITNA
                     GTEISKVVTITAVTGESAGRKEITAICVPTGTAGFEVGRAYRKMGWHASDTRELSFTD
                     CRVPLANQVGERGHGFRNFLKILDDGRIAIAALAVGLAQGCLDESIRYAKERQAFGKA
                     IGSYQAIQFKVADMAMKTRLARNQVYRAAWLKLQ"
     gene            complement(931..1614)
                     /locus_tag="VI541_04735"
     CDS             complement(931..1614)
                     /locus_tag="VI541_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70242.1"
                     /translation="MGLFRMARRVFYVFLVTLPLLYGGAEVAAKRLAEKSLAENAKSH
                     DPVATETSASVSSPLLFGLITKKTIDRVEVSANHVKLEQLLADDATVILHGVHIDVAQ
                     SMKQSEPVISSIDRMDVTFSLSQDEVSKLVPAGLRVQFAGQGVTNLVGPGFLVRGTLE
                     VEGGALAFRPLTPLPKGVRNLRLPLGETVASCIQNIEIQVGKAIITCSKDNPPTSFPG
                     FPGKTFGSN"
     gene            complement(1683..2342)
                     /locus_tag="VI541_04740"
     CDS             complement(1683..2342)
                     /locus_tag="VI541_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70243.1"
                     /translation="MTDPQGEQEIFEAWPPQRPRRRRRASGGRVVFGVVLIILGVGAL
                     LRQIFPIDASFVPLAIGVGFLSVWVLGPRIPGFLITGGVMTGVGAGIVGRSVLTGRLR
                     DAAMLVGLGLGFTLIGALSRRGRWAFIVGGILLLVGFVQAGFGVGSYLPGSFVRIALP
                     TVVILVGVLLLTRHLMARSVFRWLLFLLIAIAALLSTSSDQVDHSRTFPGGVVLNIKS
                     A"
     gene            complement(2377..>2956)
                     /locus_tag="VI541_04745"
     CDS             complement(2377..>2956)
                     /locus_tag="VI541_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF70244.1"
                     /translation="NEDDFEVVGEAADGEEAVARVRSSTPDVVLMDVRMPRMDGVEAT
                     RRLKDVSDAKVLILTTFDLDEYVFEAVRAGASGFLLKDVPPDDLVDAIRIVARGDALI
                     EPRMTRKLLDEFARSSTRSRSRPTGLDQLTSRESEVLALVAKGLSNSEIAEALFISDT
                     TVKTHVTHILTKLNLRDRVQAVVLAYEAGLVS"
BASE COUNT          614 a          943 c          850 g          549 t
ORIGIN      
        1 cctgcaactt cagccatgcc gcgcgataga cctggttacg ggcgaggcgc gtcttcatcg
       61 ccatgtcggc gaccttgaac tggatcgcct gataggagcc gatcgccttg ccgaaagcct
      121 gccgctcttt ggcataacgg atgctctcgt cgaggcaccc ctgggcaagt cccaccgcga
      181 gtgctgcgat ggcgatgcgt ccgtcatcga ggatcttcaa gaagttgcgg aagccatggc
      241 cccgctcgcc cacctgattc gccaacggaa cacgacaatc agtgaaggag agctcgcgcg
      301 tgtcggaagc gtgccaaccc atcttgcgat atgcgcggcc gacttcgaac cccgcagtgc
      361 cggtgggaac gcagatcgcg gtgatctcct tgcgccctgc actctcaccc gtcaccgcgg
      421 tgatcgttac gaccttagat atctcggttc cggcattggt gatgaaggcc tttgcaccgt
      481 tgataaccca gtcatcgccg tcgatacggg ctgtagtctg cgtacttccg gcgtcggagc
      541 cgccgccagg ttcggtgagc ccgaatccgc cgaggatctc gccacgggcc atgggaagga
      601 gccactgcat cttctgctcc tcggttccga agagaaagaa gggcatcgca ccgagaccca
      661 cgccagcttc gagagtgatc gccacagatg aatccactcg ggccaactcc tcgatcgcga
      721 cgcagagggc gaggaaatcg ccacccatgc ccccgtactc ctctgggaac ggaagaccga
      781 acaggcccat cttgcccatc tctttgacga tctcgagtgg gaactcggca cgttcatcga
      841 atccctcggc ttgagggcgc acgacctctt cggcgaatgc gcggaccgtc ttgcggagtt
      901 cttcgacctc tactggaaga tcgagatcca tcagttgctc ccgaacgtct tacccggaaa
      961 ccctgggaag ctcgtcgggg ggttgtcctt cgagcaggtg atgatcgcct taccgacctg
     1021 gatctcgatg ttctggatac agctggccac cgtttcgcca agcggtaggc ggaggtttcg
     1081 aactcccttg gggaggggag tcagaggacg gaacgccaaa gcgcctcctt ccacctcgag
     1141 ggttcctcga acgaggaatc ccgggccgac taggttggta acgccctgtc cggcgaactg
     1201 cacacgcaag cccgctggca ccagcttgct cacctcgtcc tgggaaaggg aaaacgtgac
     1261 atccatccta tcgatagaag agatgacggg ttcagactgc ttcatcgact gggccacgtc
     1321 gatatgcacg ccgtgcagga ttaccgtcgc gtcgtcggca agcagttgct cgagcttcac
     1381 atgattcgcg gagacctcta cgcgatctat ggtcttcttc gtgatcaggc cgaatagaag
     1441 ggggctgctg accgacgcag aagtctcggt cgccactggg tcgtgcgact tggcgttctc
     1501 ggccagcgac ttctcggcca gcctcttggc cgccacctcg gcccccccat acaaaagagg
     1561 aagagtcacc aggaacacat agaagacccg tcgggccatc ctgaacaaac ccatcagagc
     1621 tgacgcatct cgttcatgcg gcggaatcct aactgagtgc aggcgcagca gccgttcagg
     1681 ggtcaggcac tcttgatgtt gagcacgact cctcctggga aagtacgcga atgatcgact
     1741 tgatcgctcg acgtggaaag cagcgcggcg atcgcgatca ggaggaagag gagccaacga
     1801 aagaccgagc gcgccatcag gtggcgggtc agaagaagta ctccgacgag aatcaccacc
     1861 gttggaagtg cgatacggac gaagctgccg ggcagataac tcccgacacc gaatcctgcc
     1921 tggacgaaac ccaccaacag caggatgcct ccaacgatga aagcccacct gccccttcgt
     1981 gacagcgctc cgataagcgt gaagcccaag cccaggccga ccagcatcgc ggcatcgcgg
     2041 agccttcctg ttagaacgct ccgcccgact atcccggccc ccacacctgt cataaccccg
     2101 ccggtgataa ggaatcctgg gatgcgaggg cccaagaccc acaccgagag aaagccgacg
     2161 ccgatggcca acggaacgaa tgacgcatcg atgggaaata tctgccgaag cagcgctcct
     2221 actcccagga tgatcagcac cactccgaag acgacacgcc cgcccgacgc gcgccttctt
     2281 cttctcggcc tctgcggggg ccaggcctcg aagatctcct gctcgccctg tgggtcggtc
     2341 atacgccttc ctttctatcg cttggcctag aggggttcac gagacgagtc cagcttcata
     2401 tgcgaggacg acggcctgga cgcgatctct gaggttgagc ttggtgagga tgtgagtgac
     2461 atgggtcttc acggtcgtat cgctgatgaa cagagcctcg gctatctccg agttgctcag
     2521 gcccttggcc accaacgcta ggacctcgga ctcgcgcgag gtcagttggt ccaagccggt
     2581 gggcctcgac ctcgaacggg tcgaagaacg ggcgaattcg tcgagcagtt tcctggtcat
     2641 ccttggctct atcaacgcgt cgcctcgcgc cacgatcctg atcgcatcga ccaggtcgtc
     2701 gggcggaacg tccttgagaa ggaatccgct ggcccccgcc ctaacggcct cgaatacata
     2761 ctcatcgagg tcgaatgtgg tgaggatcaa gaccttcgca tcggagacgt ccttgaggcg
     2821 tcgagtggcc tccactccgt ccatccttgg catccgaaca tccatgagca ccacatcagg
     2881 ggtcgatgac cggacccttg cgaccgcctc ttcaccatct gcggcctcgc cgacaacctc
     2941 gaagtcatcc tcgttc
//
LOCUS       DATDOZ010000234         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2819561, whole genome shotgun sequence.
ACCESSION   DATDOZ010000234 DATDOZ010000000
VERSION     DATDOZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819561"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VI541_04750"
     CDS             <1..491
                     /locus_tag="VI541_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLF70245.1"
                     /translation="SLFESLEIPVKVFTSYGATEAKVPEIVDAIVDVTETGSTLRRNG
                     MRIIHTLLESPTLLIANPKSMKDPDKRVAIEEIKILLMGAINARGKVLVKLNVDEARL
                     EKVVALLPALKAPTVSRLAGEGSYAVETVVPRSEINILIPVLKAAGASDIIELPITKI
                     VD"
     gene            574..774
                     /locus_tag="VI541_04755"
     CDS             574..774
                     /locus_tag="VI541_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLF70246.1"
                     /translation="MAQGTVKWFSNEKGFGFIAQADGEDVFVHYTAITGEGYKSLTEG
                     QSVEFDVVDSQKGKQASNVRPL"
     gene            962..1537
                     /gene="infC"
                     /locus_tag="VI541_04760"
     CDS             962..1537
                     /gene="infC"
                     /locus_tag="VI541_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HLF70247.1"
                     /translation="MAAEPRLNDRIRVSQVRVVGPAGEQMGIMATADALSRAQTLDLD
                     LVEVAPAAEPPVVKIMDYGKFRYEQDIKAKESRKKQQQVNVKEMKFRPKISPHDYETK
                     KHHVMRFLGQGSKVKITIMFRGREMAHTEIGASLLKQLAEELNETSSVELLPKLDGRN
                     MIMVLAPMKRIDKKPSSMDGAEGQHAKDENS"
     gene            1515..1709
                     /gene="rpmI"
                     /locus_tag="VI541_04765"
     CDS             1515..1709
                     /gene="rpmI"
                     /locus_tag="VI541_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HLF70248.1"
                     /translation="MPKMKTHRGAAKRFRKTGTGKLVRVQGHVKHYLEHKPGTLRRRL
                     QRNGLVSKADAPRIRKMLGG"
     gene            1713..2075
                     /gene="rplT"
                     /locus_tag="VI541_04770"
     CDS             1713..2075
                     /gene="rplT"
                     /locus_tag="VI541_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLF70249.1"
                     /translation="MARVKRGVHKRKHRRKVLEAASGYRGARSRRFKAAKEQLLHSMT
                     YAYRDRKDRKGQFRRLWIVRINAAARANGISYNRFIEGLHKAEVELDRKSLAEIAVSD
                     PDAFKALVDIAKQNASVV"
BASE COUNT          524 a          634 c          711 g          422 t
ORIGIN      
        1 aaagcctctt cgaatcgctt gagatcccgg tcaaggtgtt cacgtcatat ggagccacag
       61 aggcgaaggt tcccgagata gtcgatgcga tcgtggatgt gactgagacc gggtcaaccc
      121 ttcgtcggaa cgggatgcgg atcattcaca ctctgctcga atcgcccacc cttctgatcg
      181 ccaatccgaa gtcgatgaag gaccccgaca aacgcgtcgc catcgaggag atcaagatcc
      241 ttctaatggg tgcgatcaac gccagaggca aggttctggt gaagctcaat gtcgatgagg
      301 cccgcctcga gaaggtggta gcactcctgc cggcactcaa ggcccccacg gtctcgcgtc
      361 tggctgggga gggctcgtat gcagtcgaga cggtcgtccc tcgctcggag atcaacatcc
      421 tgatccccgt tttgaaggca gccggggcct ccgacatcat cgaactgccc atcaccaaga
      481 tcgtcgactg accgggggcc ccagcggcgg gtattgtgcg cagggcccgt gagtgctacc
      541 ttgctcacag tcttactagt aaggggcaaa gtagtggcac agggtaccgt caagtggttc
      601 agtaacgaga agggtttcgg attcatcgcc caggccgatg gagaggacgt cttcgtccac
      661 tacacggcca tcacgggcga ggggtacaag agcctgaccg aaggtcagag cgtcgagttc
      721 gacgtagtgg acagccagaa gggcaagcag gcgtccaacg tcaggcccct ctaggatcgg
      781 ccatagcatt cgacgatgcg gtcgataaaa cggatatagg cgcaggaggc tccgcttctc
      841 accttctcag ccttgggctg gtaaggttat tggcacgctt cggcggcgga tcttcctgtc
      901 cgccgttttt ttgcgtcact gggaggcata tgaagagaag atctctaagg agtgatgcct
      961 gatcgccgct gagccacgac ttaatgatcg gatacgagtt agtcaggttc gtgtggttgg
     1021 tccggccgga gagcagatgg ggatcatggc caccgcggat gcactaagca gggcccagac
     1081 cctagacctc gatcttgtcg aggtcgctcc ggcggcagag cctcccgtcg tcaagatcat
     1141 ggactacggg aagttccgct acgagcagga catcaaggcc aaggagtctc gcaagaagca
     1201 gcagcaggtc aacgtcaagg agatgaagtt tcggccgaag atctcgcctc acgactatga
     1261 aacgaagaaa catcatgtga tgcgattcct cggccaaggc tccaaggtga agatcaccat
     1321 catgttccgt ggcagagaga tggctcatac tgagatcgga gctagtcttc tcaagcagtt
     1381 ggctgaagag ttgaacgaaa catcttcggt tgagcttctg ccgaagctag atggacgaaa
     1441 catgatcatg gtattggcgc cgatgaagcg tatcgataag aagccctcgt cgatggacgg
     1501 ggcggaggga cagcatgcca aagatgaaaa ctcatagggg agcagctaag cgcttccgca
     1561 agaccggcac cggcaagctc gtgcgcgtcc agggtcatgt gaagcactac ctggaacaca
     1621 agccggggac cctgcggcga cgactccagc gaaacgggct cgtctctaag gccgatgccc
     1681 cgcggatccg caagatgctg gggggctgag agatggcacg tgtgaagagg ggcgttcaca
     1741 agcgtaagca ccggcgcaag gtgctcgaag cagcgtccgg ttaccgaggc gcgcgaagca
     1801 gaaggttcaa ggcggcgaag gagcagctgc tccactcgat gacctatgcg tatagggacc
     1861 gcaaagatcg caagggtcag ttccgccgac tctggatcgt tcgcatcaac gcggccgctc
     1921 gcgccaacgg catctcatac aaccggttca tcgagggtct ccacaaggcc gaggtcgagt
     1981 tggaccgcaa gagcctcgcc gagatagctg tctcggaccc ggatgcattc aaagcgttgg
     2041 tggacatcgc gaagcagaac gcttccgtag tctgaccgac ccctgataac cagcccgaga
     2101 aacgctgacg tagtcagggc ccgcaagctc gcccgcaagg gtatccgtga ccgcagccgc
     2161 gagttcatcg tcgagggccc gacctgtttg acggaagcgc ttgcggcggg tcgttcacct
     2221 gagatggtct tcttcgtagc cgggaccggt gaccatgagg ccctcatcgc gtctatcaag
     2281 ggatccggaa c
//
LOCUS       DATDOZ010000235         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3893774, whole genome shotgun sequence.
ACCESSION   DATDOZ010000235 DATDOZ010000000
VERSION     DATDOZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893774"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VI541_04775"
     CDS             <1..1085
                     /locus_tag="VI541_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLF70250.1"
                     /translation="VIGGIPVGGMHIDDARETLREKFEDPLDRTVSLEANGQHWDTSA
                     RALGASSDLDERLSGIGINEGSTVVSRLWRRVTELRGERVLAVHVRVDRSRLDSSIDE
                     FARTVDIAATSASPTLENGVLRIHPAVQGFELDRGAAVTQLNTALRSGESVVHLAGHV
                     IAPPSSAVDDVLVVRIGPNVLERYVGESLVKTYRVATGSSEFPTPVGTFKVINRRSKP
                     TWVNPAKFPGGWGWDLPASIPPGPGNPLGTRALDLNVSGIRIHGTYANYSLGYNASHG
                     CIRMAIADVEELFDQIRVGTPVFITRSGALKPLRGRRPPPAAPSAENDASLVPGSGEP
                     VASPSPTPSPSPSSPAPTPPDTPPGL"
     gene            1137..2249
                     /locus_tag="VI541_04780"
     CDS             1137..2249
                     /locus_tag="VI541_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HLF70251.1"
                     /translation="MFPLSKGKVTKQAHVALPGGTFEDEHGREAFAGRASHLYRTHPP
                     TAWVRIDGPLRPHALDLNEVKTSDAIDPDGVWTKILASARCRIYISRRSEAMPYFLRD
                     ADGDICYFVHRGAGVMESDYGPLTYGPGDFLIVPKGTTHRFLPDGTDGFILVVEGTGE
                     YSIPDRGLLGRHAQFDPGVIVTPEPEPHDEVGEFEVRVKRDDTYTSLFFKWHPLDVVG
                     WQGDLCPMKLNVKDYRPIVSPRYHLPPSAHCTWQNDGFAVCTFAPRPLEEDPDVLRVP
                     FYHANIDNDEVIFYHEGEFFSRPGIGAGKLTLHPQGIHHGPQPAAIDAVGGKKRTDEV
                     AIMVESEDPLELTPEAEQVRDISYETSWARGMGLFD"
BASE COUNT          451 a          721 c          721 g          458 t
ORIGIN      
        1 tggtgatcgg agggatcccc gtcggtggga tgcacatcga tgacgccaga gagaccttga
       61 gagagaagtt cgaggatcca ctcgaccgaa cggtgtcgct cgaggccaac ggccagcatt
      121 gggatacatc cgcgcgagcc ctaggtgcat cttcggacct ggatgaacga ttatctggga
      181 tcggcatcaa cgaaggctcg actgtggtca gtcggttgtg gcgacgcgtt acggaactgc
      241 gaggcgagcg cgtcctggcc gtccatgtga gggtcgatcg ttcgcgtctc gactcgtcga
      301 tcgacgagtt tgccaggacc gtagacatcg ccgcgacctc tgcttcacct actctcgaaa
      361 acggtgtgct gcggatccat ccagcagtgc aaggattcga gttggatcgt ggtgcggcgg
      421 tgacccaact gaacacggcc ttgcgttctg gagagagcgt cgtgcatctg gctggtcacg
      481 tgatcgctcc accttcgtcg gcggtcgacg acgtactcgt cgtgaggatc gggccaaatg
      541 ttctcgagcg ctatgtcggc gagtcgctgg tcaagaccta ccgcgtagcg acgggttcga
      601 gtgagttccc cacgccggtc ggtacattca aggtcatcaa tcgaaggtcc aaacccacct
      661 gggtgaaccc tgcgaagttt ccagggggct ggggctggga cctgcccgcc agcatcccgc
      721 cagggcccgg gaatcctctc ggaactcgtg ccctcgatct gaacgtgtcg ggcatccgga
      781 tacacggtac atacgcgaac tactcgctcg ggtacaacgc ctcgcatggc tgcatccgca
      841 tggcgatcgc cgacgtagag gaactcttcg accagattcg cgttggaacg ccggtcttca
      901 tcacccgttc tggagccctc aagcccctta ggggtcggag gcctccgccg gcggccccca
      961 gcgccgagaa cgatgcaagc ttggtccccg gtagtgggga gcctgttgct tccccttcgc
     1021 ctacaccctc tccatctccc tcatcgcccg caccgacccc accagatacc cctccaggct
     1081 tgtagcatcg gagggatccg agtgatccgc aacgttacga acctaaggag ggtctagtgt
     1141 ttcctttatc gaagggcaaa gtcaccaagc aggcccatgt tgccctgccg ggcggaacgt
     1201 tcgaagacga acatgggaga gaggccttcg cgggccgcgc ctcgcatcta tatcgaacgc
     1261 atcctccgac cgcatgggtg cggatagatg ggccgctgcg accgcacgcg cttgatctca
     1321 acgaagtgaa gacatcagac gcgatcgacc ccgatggcgt ctggacgaag atcctcgcaa
     1381 gtgcaagatg tcgtatctac atctcgcgtc ggagcgaggc catgccctac tttcttagag
     1441 atgccgacgg agacatctgt tacttcgttc atcggggggc gggagtgatg gagagtgact
     1501 acggcccgct tacatatggg ccgggtgact tcttgatcgt ccccaaagga accacgcatc
     1561 gattcctgcc cgatgggacc gatggattca tcctcgtggt cgaggggacc ggggagtatt
     1621 cgataccaga ccgcggtctg ctcggccgac atgcgcagtt cgaccccggc gtcatcgtca
     1681 cgcccgagcc cgaacctcac gacgaggtcg gcgagttcga ggtccgggtc aagcgcgatg
     1741 acacctacac ctcgctcttt ttcaaatggc atccgctcga tgtggtcggg tggcaggggg
     1801 acctctgccc gatgaagttg aacgtcaagg actatcgccc catcgtgtcg ccgcggtacc
     1861 accttccgcc gtcggcccac tgcacttggc agaacgacgg gttcgctgtc tgcaccttcg
     1921 ctccgcgtcc gctcgaagag gaccccgatg tgctgcgcgt tcccttctac cacgcaaaca
     1981 tagacaacga cgaggtgatc ttctaccacg agggcgagtt cttcagtcgg cccggcatcg
     2041 gtgcgggcaa attgacgctt caccctcagg ggatacatca cggtccccaa ccggcggcca
     2101 tcgacgcggt cggcgggaag aagcgaaccg acgaggtagc gatcatggtc gagtcggagg
     2161 atcccttgga gttgacgcca gaggccgagc aggtgcgcga catctcgtat gagacatctt
     2221 gggcacgcgg gatgggactc ttcgactgac ctatcgtctg agccttcaga cgatgctgtt
     2281 gcgcaggatg cctataccgg tgacctcgag ttccacgacg tcgccgggtt gcaggtacgg
     2341 gccaccctgg t
//
LOCUS       DATDOZ010000236         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_985273, whole genome shotgun sequence.
ACCESSION   DATDOZ010000236 DATDOZ010000000
VERSION     DATDOZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985273"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VI541_04785"
     CDS             <1..511
                     /locus_tag="VI541_04785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF70252.1"
                     /translation="GEYSTGRKGLPLLIEALKGEPLALIVVKGPGPRARVEADLARRG
                     MAQRVMFIEPGLGVASVMAAADVVVVPSTYEPFSLVALEAISSGRPLVISSRAGATEL
                     VSSVAKVVDPLDTEALRTAIMEVKNHPPDRMLIEEGIAIAERNSWTTVGERSVDVIER
                     LASEHRVGR"
     gene            508..1227
                     /locus_tag="VI541_04790"
     CDS             508..1227
                     /locus_tag="VI541_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLF70253.1"
                     /translation="MKQDMPVVILCGGRGTRLGKETLPKGLIEIGGRPILWHIMKLYA
                     AQGFERFILCLGFGAEAITAAFSRSDSLADPSWRIEFADTGLDTPTGGRIRKVAHMID
                     GPFMATYGDGVAQIDLRDLLEHHRTQGRYATITTARARMPFGLVHIDEDSSVTGFSEK
                     PRLDEWINGGFFVFEPEVIDYLDEDSVLETQPFEKLVADGQMTAYRLDAFWACMDTYK
                     EALMLNELWDTDPPWRIWDAP"
     gene            1248..>2032
                     /locus_tag="VI541_04795"
     CDS             1248..>2032
                     /locus_tag="VI541_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF70254.1"
                     /translation="MSGFDWKGTGVFVTGATGFVGGWLATRLVELGARVVALVRDVDP
                     LAMKVHPQLWDSAVQVSGDVTDLAAVRRALAEHGVTVCFHLAAQAAVRSALADPSGTF
                     ESNVKGTWTVLEACRQVSSIDRIVMASSDKAYGDQPELPYVEDVSPLAGKFPYDASKA
                     AGDIITNSYFHTFELPVAITRNANTYGGGDLSGHRLVPETVMAILGGRPPVIRSDGSP
                     ERDYLHVSDAVAGYIALAEQLGRPEIRGQAFNLGAGVAVSVIDL"
BASE COUNT          381 a          605 c          650 g          396 t
ORIGIN      
        1 gggggagtac tcgaccgggc gcaagggctt gccactgttg atcgaggctc tcaaaggtga
       61 acctttggca ttgatcgtcg tcaaggggcc cggcccccgt gcgcgggtcg aagcagatct
      121 ggccaggcgg ggcatggctc agagagtcat gttcatcgag cctggcctgg gtgtggcgtc
      181 agtcatggca gcggctgatg ttgtggtcgt cccgagtacc tacgagccct tctcgctcgt
      241 ggcgctcgag gcgatatcct cgggaaggcc actggtcatc tcctcgcgag ccggggctac
      301 cgaactcgtt tcgtcagtgg caaaggtggt cgatccactc gacacagaag cgttgcgcac
      361 cgccatcatg gaggtgaaga atcacccccc cgaccgcatg ctcatcgaag aggggatcgc
      421 catcgcagag cgcaatagtt ggacgacggt cggagaacgc agcgttgacg tgatcgagag
      481 actcgcctca gaacatagag tgggccgatg aaacaggaca tgcccgtggt catcctctgc
      541 ggaggacgcg ggacgagact cggcaaggag acgctcccga agggcctgat cgagatcggg
      601 ggccgcccca tcctctggca catcatgaag ctctacgcgg cccaagggtt cgagcgattc
      661 atcctctgtc ttgggttcgg cgccgaggcg atcacggctg cgttctcgag gtccgatagc
      721 ttggccgacc catcgtggcg gatcgagttc gccgacaccg ggctcgacac tccgaccggg
      781 ggccggatcc gcaaggtcgc ccatatgatc gatggtccat tcatggccac atacggcgat
      841 ggcgttgcac agatcgacct gcgtgatcta ttggagcatc accgcactca aggacgctac
      901 gcgaccatca ccacggcgcg ggcgaggatg cccttcggtc tggtccacat cgacgaggac
      961 tcgtcggtca cgggcttcag cgagaagcca cgcctcgatg agtggatcaa cggcggattc
     1021 ttcgtcttcg agccagaggt catcgactat ctcgacgagg attccgtcct agagacccaa
     1081 cctttcgaga agctcgtggc ggatggccag atgaccgcat atcgtctcga tgcgttctgg
     1141 gcctgcatgg atacttataa ggaggccctg atgctgaatg agctgtggga taccgatcca
     1201 ccctggcgca tctgggatgc tccatgaagc gaatggaagg atagagcgtg agcggttttg
     1261 actggaaggg gaccggcgtc ttcgtaactg gggccaccgg gttcgtaggt ggctggctgg
     1321 caactcggct ggtcgaactg ggtgcccgtg tggttgccct ggtccgtgat gttgaccccc
     1381 tagcgatgaa ggtgcatccg cagctttggg actcggccgt ccaggtatcg ggtgacgtga
     1441 ccgatctagc ggcagtccgc agagccctag ccgaacatgg cgtaactgtc tgttttcacc
     1501 tcgctgccca agcggcggtc cgatcggcgc ttgccgatcc gtcggggaca ttcgagtcga
     1561 acgtgaaggg aacttggacc gttctcgagg cctgtcgtca ggtctccagc atcgaccgca
     1621 tcgtcatggc ctcgagcgac aaggcctacg gcgaccagcc agagctgccc tatgtggagg
     1681 atgtctcacc cctggcggga aagttcccat acgatgcgtc gaaagcggct ggggacatca
     1741 tcaccaactc ctacttccac accttcgaac tccccgtcgc gatcactcgt aacgccaaca
     1801 catacggggg tggcgacctg tccggtcatc gccttgtccc cgagaccgtg atggcgatac
     1861 ttgggggcag gcccccggtc atccgcagcg acggttcgcc cgagcgcgac taccttcacg
     1921 tctctgatgc tgtggccggt tacatcgcgc ttgccgagca gttggggcgc cctgagatca
     1981 gaggccaagc attcaacctc ggtgccggtg ttgcggtgag tgtcatcgac ct
//
LOCUS       DATDOZ010000237         5134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3064390, whole genome shotgun sequence.
ACCESSION   DATDOZ010000237 DATDOZ010000000
VERSION     DATDOZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064390"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1747
                     /gene="tig"
                     /locus_tag="VI541_04800"
     CDS             392..1747
                     /gene="tig"
                     /locus_tag="VI541_04800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HLF70255.1"
                     /translation="MSYYSPTMKTIIEEEGPTRRKLLIEVEPDEYRPLVDTTLRRLAT
                     EVKVPGFRKGKVPRAILEGRLGREAIREEFLRDAVPSLYTKAAREESLKAVSYPDIEV
                     ISFVEGEQLRFSATVDVRPEITLPICKGIKVRRPPTDATPEEVDAQLDRLREHLGILE
                     PAGRAAAAGDHVTIDIYATREQERIDSTVAEGLMYEVGSGGLVPELDAKLEGSNIGDE
                     IEFDATLPDRFGPPHAGQDVTFHVTVRDIQIRRLAELDDAFAKTASEFDTLEELRSDI
                     ATRIEEVRSVEADGAVRQLVLADLLERVDVPVPASISAHETEHRLTALLRDLERAGVT
                     LERYLEANETTEDALVENYRKVAERNVAADLVLDAIVKQENLELEREEFAAEIASLAQ
                     RSGREPDDLLKELASSGRVEVLAGDILRRKALEFLVENAEIETEPLMGTESSEVSHES
                     E"
     gene            1734..2372
                     /locus_tag="VI541_04805"
     CDS             1734..2372
                     /locus_tag="VI541_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLF70256.1"
                     /translation="MSQNEFSNYLVPIVIEQTNRGERSYDIYSRMLNERIIFLGTPVD
                     DAVANLVAAQLLFLESQDSDKDIWIYINSPGGSITALLAIYDTIQFVKPDVCTLVMGM
                     AASAAAVILSAGAKGKRFATPNSTILIHQPSGGAQGQAIDIEIQAKEILRMRSLLDEI
                     LSRHTGQTLEKVSRDSDRDFIMTAEQAKDYGMIDEIISKREQTQGLDNLKTS"
     gene            2392..3666
                     /gene="clpX"
                     /locus_tag="VI541_04810"
     CDS             2392..3666
                     /gene="clpX"
                     /locus_tag="VI541_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLF70257.1"
                     /translation="MPKFGEGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEDQTDGAELKLEELPKPKEIYEFLNDYVVGQEQAKKVLSVAVYNHYKRVQADTKH
                     DDEVELQKSNILLLGPTGCGKTLLAQTLARMLNVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADYDVKKAETGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPHQEFIQIDTSNVLFICGGAFAGLEKIIESRVGKRGMGFGGDVRKMEE
                     KDLGSILAQVLPEDLLKFGLIPEFVGRLPVISHVHNLDRVALIEILTQPKNALVKQFI
                     KTLQLDSVELEFSEGALAAIADQAMLRGTGARGLRAIMEEVLLDVMYDLPSRKDIIRC
                     VIDQDTVLEKVNPTLVPRVVEKPKRERGERSA"
     gene            3810..>5134
                     /gene="valS"
                     /locus_tag="VI541_04815"
     CDS             3810..>5134
                     /gene="valS"
                     /locus_tag="VI541_04815"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLF70258.1"
                     /translation="MEAAPKAYEPALVEDRIYAMWEQGGYFHAEPDPSRKPFCIAIPP
                     PNVTGSLHLGHALDNTLQDVLIRFKRMQGYCTLWIPGTDHAGIATQNVVEKDLAKEGL
                     TRHDLGRERFVERVWEWKQLYGDTIIRQLRRLGSSCDWSRERFTMDEGLSKAVRKVFV
                     DLYDEGLIYRGNRIINWCPRCETALSDIEVNHVETVGELAHFTYRLVDGDGDLTVATT
                     RLETMLGDTAIAVHPEDARYKRFVGRAVIHPFDGRPIPIVADEAVEPEFGTGAVKVTP
                     AHDPTDFEIAQRQGLPMLNIFDGRAHVNENGGKYAGLERYEARRRVREDLIELGLFGG
                     AFEHTYSLGHCDRCDTVVEPWLSEQWFVSMQPLAEPAIAAVRHGRTRFHPERWTNFYL
                     NWMEQIRDWCISRQLWWGHRIPVWYCDSCDHVWASLEDPTACAKCSSSD"
BASE COUNT         1122 a         1470 c         1537 g         1005 t
ORIGIN      
        1 cggcgattgt tgcttcgccg cctggggcgt gagcgaatgc tccaccgata acaactgtgc
       61 tcgccatgac agccagggcg agaagtgcga tgcgacgctt catgtgttgc tcctttccct
      121 tggtacggat ccccccgtac cactcttctg ttaccggttt tccggtcgtg gggatagtgt
      181 tcgatgcgcg tttgcgaatt cctcttcacg ttacgcgtat ttccctcaac tttgcggggg
      241 atacgttcaa tggtgcaagt tactcgcgct atgcgcgccc tacgtctctt tcactagtgc
      301 atcgtcacta acgatttcca tcgtttccgt ttctgccgaa aatggcaccg gatgaagcgg
      361 aagaaacgac caacgggttg gcggccaaga ggtgagttac tattcgccga ccatgaaaac
      421 gatcatcgaa gaagaaggtc caactcgacg aaaactcctc atcgaggtcg agcccgatga
      481 gtatcgaccg ctcgtcgata cgacgttgcg gcgccttgca accgaggtca aggtacccgg
      541 attccgaaaa ggcaaggtcc ccagggcgat ccttgagggc cgcttaggga gagaagccat
      601 cagggaagag ttcctgcgcg atgctgtgcc ttccctatac accaaggccg cccgcgagga
      661 gtcgttgaag gcggttagct atccagacat cgaggtcatc agcttcgtcg agggggagca
      721 actccgattc tcagcgacgg ttgatgtcag gcccgagatc acgctcccta tatgtaaggg
      781 gatcaaagtc aggaggcccc caaccgatgc aacgcccgaa gaagtggacg cccagctcga
      841 tcgcctacgc gaacacctgg ggatcctcga gccggctgga cgcgcggctg ccgctggtga
      901 tcacgtgacc atcgatatct acgcaacgag agagcaggaa cggatcgact cgacggtcgc
      961 cgagggtctg atgtatgagg ttggctcggg cggcctggtc ccggagctcg atgcgaaact
     1021 cgaggggtcc aacatcggcg atgagatcga gttcgatgcc acactgcccg acaggttcgg
     1081 ccccccacac gcaggacagg atgtgacctt ccatgtcacg gtgcgcgaca tccagatccg
     1141 caggctagcg gaactcgatg atgccttcgc taagacggca tctgagttcg acaccctcga
     1201 agagttgcgg tccgacatcg cgacgcggat cgaagaggtc cgcagcgtcg aggcagacgg
     1261 cgccgttcgt caacttgttc tcgccgatct tctagagcga gtggatgttc ccgttccggc
     1321 ttctatctcg gcccacgaaa ccgaacatcg gctcactgcc cttctccgtg atctcgagcg
     1381 ggcaggcgtg actctcgagc gctaccttga ggcgaacgag acaacggaag atgcattggt
     1441 cgagaactat cggaaagtgg ccgagcgcaa cgtggcggcg gatctcgtcc tcgatgcgat
     1501 cgtgaagcag gagaacctag agttagaacg ggaagaattc gcagcggaga tagcttctct
     1561 tgcccagcga tctggccgag aacccgacga tctgctcaag gagttggctt cgagcggtcg
     1621 agtcgaagtt ttggccggtg atatcctgcg aaggaaggcc ctcgagttct tggttgagaa
     1681 cgccgagatt gaaacagagc ccctgatggg gaccgagtca agtgaggtta gccatgagtc
     1741 agaatgagtt cagcaactac ctggtcccca tagtcatcga gcagaccaac cgtggcgagc
     1801 gctcttacga catctactcg cgcatgctca atgaaaggat catcttcctg gggacccccg
     1861 tcgatgacgc cgtcgccaac ctcgtcgccg cacagttgct cttcttggag tctcaggatt
     1921 ccgacaagga catctggatc tacatcaact cgcccggtgg cagcatcact gcactgcttg
     1981 cgatctacga caccatccag ttcgtcaagc ccgatgtctg cacgttggtc atgggcatgg
     2041 ctgcaagtgc tgctgctgtg atcctgtcgg cgggggccaa ggggaagcgc ttcgcaactc
     2101 ccaactcgac catattgatc caccagccga gcggtggtgc gcagggacag gccatagaca
     2161 tcgagatcca ggcgaaggag atcctccgga tgcgttccct gctcgatgag atcctctcgc
     2221 gccataccgg ccaaacgctt gagaaggtct cgcgcgatag tgaccgtgac ttcatcatga
     2281 ccgctgagca ggctaaggat tacggtatga tcgatgagat catcagcaaa cgagaacaga
     2341 ctcagggtct cgataacttg aagacttctt agtagaggca ggagacagag cgtgccgaag
     2401 ttcggcgaag ggggcgacct cctcaagtgc agcttctgcg gtaaatctca gaagcaggtc
     2461 aagaaactca tcgccgggcc tggcgtctat atctgtgacg agtgcatcga tctgtgcaac
     2521 gagatcatcg aagaagacca gaccgatggt gcggagttaa agctcgaaga gcttcccaag
     2581 ccgaaagaga tctacgagtt tctcaacgac tatgtggtcg gccaggaaca ggccaagaag
     2641 gttctctcgg tagctgttta caaccactac aagcgggtac aggccgacac taagcacgac
     2701 gacgaagtgg aacttcagaa gtccaacatc cttctgctcg ggccgacggg gtgcggcaaa
     2761 accctcctag ctcaaacctt ggctcggatg ctcaacgtcc cgttcgcgat cgcagatgca
     2821 actgccctga ccgaggctgg ctatgttggc gaggatgtcg agaacatcct gctgaagctg
     2881 atccaggcgg cggactacga cgtgaagaag gccgagaccg ggatcatcta catcgatgag
     2941 atcgacaaga tcgcccgcaa atccgagaac ccttccatca cccgcgatgt atcgggcgag
     3001 ggcgtgcagc aggctctgct gaagatcctc gagggaacca cagcaagcgt gcctccgcag
     3061 ggtgggcgta agcacccgca ccaggagttc atccagatcg acacgtctaa tgttcttttc
     3121 atctgcggag gagcattcgc cgggctcgag aagatcatcg aaagtcgagt cggtaagcga
     3181 ggcatgggtt tcggcggtga cgtccgcaag atggaggaga aggacctggg ctcgatactg
     3241 gctcaggttc tacccgagga cctgctgaag ttcggtctga tcccagagtt cgtcggtcgg
     3301 ctcccagtca tctcgcacgt tcacaaccta gacagagttg ctctcatcga gatcctcacc
     3361 caaccgaaga acgctctggt caagcagttc atcaagacgc ttcaactcga tagtgtcgag
     3421 ttggagttct cggaaggtgc tcttgcagcc atcgccgacc aggcgatgtt gcgcggcaca
     3481 ggggcacgtg ggctcagagc gatcatggaa gaggttcttc tcgacgttat gtatgacctg
     3541 ccgtcgcgca aggacatcat ccgatgtgtc atcgatcagg acaccgttct cgagaaggtc
     3601 aaccccaccc tggtcccgag ggttgtcgaa aagcccaagc gcgagcgggg cgagcggtcg
     3661 gcctagcctc tcctgtgatt gaatgtctat tgatgcaaac gacgcccgta tcagtagacc
     3721 ggggaacctg gggggcttag ccaccctccg aggttcccag cccccgcgcc ataagcgggg
     3781 ttttctattt tcagggtaga gaggatgaga tggaagcagc gccgaaagcc tacgaacctg
     3841 cgctggtcga ggaccgtatc tacgccatgt gggagcaggg tggttacttc cacgcggaac
     3901 cagacccatc gcgcaagccc ttctgcatag ccataccgcc cccaaacgtc accgggtcgc
     3961 tccacctcgg gcacgcactc gacaacacac tgcaggatgt cttgatcagg ttcaagcgga
     4021 tgcagggtta ctgcacgctc tggatccctg gaactgacca tgctggcatc gcaacgcaga
     4081 acgtggtcga gaaggatctc gcgaaggaag gcctcacgcg ccacgatctc gggcgcgagc
     4141 gcttcgtcga gcgcgtctgg gagtggaagc aactctacgg cgacacgatc atccgtcagt
     4201 tgcgcaggct tggatcgtcg tgtgactggt cgcgcgagag attcaccatg gacgaaggcc
     4261 tctcaaaggc ggttcgcaaa gtgttcgtcg atctctacga tgagggcctg atctatcgcg
     4321 ggaacaggat catcaactgg tgcccccgat gtgagaccgc tctgtcggac atcgaagtca
     4381 accacgtcga gacggtgggc gaacttgctc acttcaccta ccgcctcgtc gatggggatg
     4441 gcgacctcac tgttgctacg acccgactcg agacgatgct cggagacacg gcgatcgcgg
     4501 tacatcccga ggacgctcgg tacaagaggt tcgttggccg ggcggtcatc catccgttcg
     4561 acgggaggcc tatcccgatc gtcgcagacg aggcggttga acctgagttc ggaacgggag
     4621 ccgtgaaggt cacaccagct catgacccga ccgacttcga gatcgcgcag cgccagggac
     4681 tgccgatgct caacatcttc gatggccggg cccacgtcaa cgagaacggc gggaagtacg
     4741 cgggtctgga gcgatacgag gcgcgacgca gggtccgcga ggacctgatc gagctcggtc
     4801 tcttcggtgg cgccttcgag cacacatact cattgggaca ctgcgatcgg tgtgacacgg
     4861 ttgttgagcc ctggctctcg gaacagtggt tcgtcagcat gcaacctctt gccgagcccg
     4921 cgatcgcagc tgtccgccat gggcggacgc gcttccatcc cgagaggtgg acgaacttct
     4981 atctgaactg gatggagcag atccgtgatt ggtgtatctc tcgacagctc tggtgggggc
     5041 accggatccc ggtctggtac tgcgattcct gtgaccacgt gtgggccagc ctcgaagatc
     5101 cgacggcgtg cgcgaagtgc tcttccagcg atat
//
LOCUS       DATDOZ010000238         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3587018, whole genome shotgun sequence.
ACCESSION   DATDOZ010000238 DATDOZ010000000
VERSION     DATDOZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587018"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VI541_04820"
     CDS             complement(<1..619)
                     /locus_tag="VI541_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLF70259.1"
                     /translation="MDVIIEEGPIGLLTIDRADRRNALTIETTMQIAAGITKLAASSR
                     VIILTGAGSAFCAGGDFDELKRMAGKPAKEAADMLYSGFQSMILAIRRVDIPVIAAIN
                     GHAMGAGMDLALACDLRVASRSAKLGQVWVKVGIIPGTGGAFWVSLLAGAGRAAQMIL
                     SGEPIDATTALEWGLVNDVVDPHAVVGRARELANEIAKNPPGAVAA"
     gene            complement(648..908)
                     /locus_tag="VI541_04825"
     CDS             complement(648..908)
                     /locus_tag="VI541_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70260.1"
                     /translation="MSSEPSTTTEDIARRIRGEPQDGFEGFEVRSVERSPRSYTCPHC
                     NHEIAPMMAHVVVVPVEDVDRRRHFHGPCWRKVIESGREPPL"
     gene            complement(939..1700)
                     /locus_tag="VI541_04830"
     CDS             complement(939..1700)
                     /locus_tag="VI541_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HLF70261.1"
                     /translation="MTEQDFSKILVFCPHPDDGEFTAGGSMAKWSSQGKEVVLCVVTN
                     GAAGSNDPDVNRDWLIETRIKEQKAAAAITGISDVIFLGYEDGHVEDSHELRRDMIRE
                     MRRFKPDLVVCNDPTNWFFAQRYINHPDHRKVGEAFLAAVNPGATTVPLYREELYDKG
                     FMPHQLKACYLGFTSEPDHFVDISDQIETKVSAIMAHVTQMGDAFDRINSMVREMGSV
                     IGAMSGKGYGHAEGFRALYFDSGPGTPTGPTPNSA"
     gene            complement(1697..2389)
                     /gene="thyX"
                     /locus_tag="VI541_04835"
     CDS             complement(1697..2389)
                     /gene="thyX"
                     /locus_tag="VI541_04835"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HLF70262.1"
                     /translation="MPAVGDKIEVLDHGFVRLDAAEATDLSVVNGARVSFLQMKQQMD
                     TKDEGLIRFLMRERHASPFEHNLFRFHIRCPMAVAREWMRHRWSSFNEHSLRYSNAID
                     DFYVPEAEQVRTQVGKPGAYSFEPLPPDVALSVQDMLRKQYQDAWNLYQELLEMGLAR
                     ELARLVLPVGIYTEFYWSVNARALMNFISLRNDDAAMWEIRQFAIAVEDFFAEQMPAT
                     HTAFVEFGRVAP"
BASE COUNT          484 a          790 c          725 g          502 t
ORIGIN      
        1 tggcagccac ggcccccgga ggattcttcg ctatctcgtt ggcaagttct ctggctcgcc
       61 ccacaacagc gtggggatcg actacgtcgt tgacgaggcc ccactcaagc gccgtcgtcg
      121 cgtcgatggg ttcgccggaa aggatcatct gtgcggccct cccggccccc gcgagaagac
      181 tcacccagaa agcaccgccc gtaccgggga tgatcccgac cttcacccag acctgaccga
      241 gttttgcgga cctcgacgct acgcggaggt cgcacgccag agcgaggtcc atccctgctc
      301 ccatcgcgtg accattgata gccgcgatca ctgggatatc cacacgtctg atggcgagga
      361 tcatgctctg gaaccctgaa tagagcatgt cggccgcttc cttcgcaggt ttgccagcca
      421 tacgcttcaa ctcgtcgaag tcaccgccag cgcagaaggc cgagcctgca cccgtcagga
      481 tgatcactcg cgaggacgca gccagcttcg tgatgcccgc cgcgatctgc atcgtcgtct
      541 cgatggtcag ggcattgcgc cggtccgcgc ggtcgatcgt cagcagaccg atcgggccct
      601 cttcgatgat cacatccata ccagcaaacc tactagaaaa ggaacttcta cagaggcggc
      661 tctcgtccgg actcgatcac ctttctccag caggggccgt ggaagtgacg acgccggtcg
      721 acatcctcaa ctggaacgac gacgacatgg gccatcatgg gtgcgatctc gtgattgcag
      781 tgagggcagg tgtaactacg aggactccgc tcgaccgacc tgacctcgaa cccttcgaac
      841 ccatcctgag gttcaccacg gatccgtctg gcgatatctt cggtagtggt agagggctcc
      901 gagctcatgc tgcgcttggg cttgcggcgc ggcacgcgct acgcgctgtt cggggtcggc
      961 cccgtggggg tgccggggcc agagtcgaaa tacagggccc tgaacccctc ggcatggccg
     1021 tatcccttcc ccgacatcgc tccgatcaca gaccccatct cacgcaccat cgagttgatg
     1081 cggtcgaagg catcgcccat ctgcgtcacg tgagccatga tcgccgagac cttcgtctcg
     1141 atctggtcgc tgatatcgac gaagtggtcc ggctcggagg tgaaaccgag gtagcaagcc
     1201 ttgagctgat ggggcatgaa ccctttgtcg tacaactctt ccctgtacaa gggaaccgtc
     1261 gtcgcgcccg ggttcaccgc ggccaagaag gcctcgccga ccttgcgatg gtcgggatgg
     1321 ttgatatagc gctgtgcgaa gaaccagttg gtggggtcgt tgcagacgac aagatccggt
     1381 ttgaacctgc gcatctcgcg gatcatgtcg cgacgcaatt cgtgcgaatc ctcgacgtgc
     1441 ccatcctcat aaccaaggaa gatgacatcg gagatcccgg tgatcgccgc ggccgccttc
     1501 tgttccttga tgcgagtctc gatcagccag tctctgttca catctggatc gttgctgccc
     1561 gcggcgccgt tcgtgaccac acagaggaca acctccttac cctgcgatga ccacttagcc
     1621 atcgacccac ctgcggtgaa ctcaccgtca tcggggtgtg gacagaagac gaggatcttg
     1681 gagaaatcct gttccgtcat ggcgctaccc ttccgaactc aacgaaggcg gtgtgagtag
     1741 ctggcatctg ctcggcgaag aaatcttcaa cggcgatcgc gaactggcgg atctcccaca
     1801 tggctgcgtc atcgtttcga agagagatga agttcatcaa tgccctcgcg ttgacggacc
     1861 agtagaactc tgtgtagatg ccaacgggca gaaccagcct ggccagttcg cgcgcgaggc
     1921 ccatctcgag aagttcctgg tagaggttcc aggcatcctg gtactgcttg cgcagcatgt
     1981 cctggaccga caacgcgaca tcaggcggca gcggctcgaa gctgtaggcc cccggcttgc
     2041 cgacctgagt gcggacctgc tctgcctcgg ggacgtagaa gtcgtcgatg gcgttggagt
     2101 acctgaggct gtgctcgttg aaactgctcc atcggtgacg catccactcg cgcgcgacgg
     2161 ccataggaca tcggatgtgg aatctgaaga ggttgtgctc gaaaggcgat gcgtgtctct
     2221 cgcgcatgag gaaacggatg aggccctcgt ccttggtgtc catctgctgc ttcatctgca
     2281 agaaggagac cctggctccg ttcacaacgg aaaggtcagt cgcctcggcc gcatcgagtc
     2341 gtacgaaacc gtggtcaaga acttcgatct tgtcacctac tgcgggcatg tcgcctctcc
     2401 ctcttttcgc agcatcctaa cccccgagtg cgcccctccg agtggcatcc gtgacatcgc
     2461 cctcctgccg gtgcacactt tccttcgtga aaggtcccct c
//
LOCUS       DATDOZ010000239         2726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1523805, whole genome shotgun sequence.
ACCESSION   DATDOZ010000239 DATDOZ010000000
VERSION     DATDOZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523805"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VI541_04840"
     CDS             complement(<1..352)
                     /locus_tag="VI541_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLF70263.1"
                     /translation="MFDETIQVSLDDLRDRMRSIHPPCVEMVDEIERLVRSGGKRIRP
                     TFCYMAFRGLGGEHCPEIILAASSLEMLHTFALIHDDIMDRSMMRRGRPSTYSTRPTD
                     AVLIGDLAFALSDSM"
     gene            complement(393..1313)
                     /locus_tag="VI541_04845"
     CDS             complement(393..1313)
                     /locus_tag="VI541_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70264.1"
                     /translation="MDRREFLRLAGGGALSVTLIRTLAGCGISGRDPENIPKAFAALA
                     DRFAGDEPGLQLFLGGQEYLANDTNRITMRIEDRESRQIESLHPSLWFSSRGRVHGPL
                     HLGLERFAPRTSSPQEGDPTSFYVGAIDLVAPGIVDLAVEVVVAGRKKVGFTALQALA
                     GGRVPGIGQPAVSTPTPVATDPGGLQGICTRIPGCPCHESSLEAALTSGKPVVFIVAS
                     PRLCKSRVCGPVLEEVLATRETHGDRAIFIHAEPYRDDSATTISAPASAWQLDSEPWT
                     WVIDSDGVIKARFEGPVIAHELSSALDEVV"
     gene            1405..2388
                     /gene="coxB"
                     /locus_tag="VI541_04850"
     CDS             1405..2388
                     /gene="coxB"
                     /locus_tag="VI541_04850"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLF70265.1"
                     /translation="MFALVVLAACSRDAPQNFLHPIGPIAKEQDGLWRLTFGIAAAVF
                     VIVEGLLVFSIFRFRARPHGAEPKQTHGNTKAEIAWTIAPVLLLTFVAFPTVSAIFKL
                     AKEPTDPLQVQVVAHQWWWEYRYEGSGVVTANELHIPVGRPVDLSLESADVIHSYWVP
                     KLAGKQDVIPGRVNHLTIQADEPGVFEGQCAEYCGFSHANMRLRVIAETESDFESWLQ
                     SQKRSARSPTDPLALEGQRLFLEGSFANGQQCSGCHAINGTTAAGTFGPDLTHLASRT
                     SFAGSMFEMTDDDLVAWLRDPPGRKPGSQMPNLKLTDSQIQALVAYLNALT"
     gene            2402..>2726
                     /locus_tag="VI541_04855"
     CDS             2402..>2726
                     /locus_tag="VI541_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLF70266.1"
                     /translation="MATVVETPRILRRPSSKTGIVGWLTTVDHKKIGILYGYTAFLFF
                     ILGGVEALLIRLQLATPDGKVLSADLYNQLFTTHGITMIFLVVMPLGAAFFNYLIPLM
                     IGARDV"
BASE COUNT          520 a          857 c          801 g          548 t
ORIGIN      
        1 acatcgagtc tgacagcgcg aaggcgaggt ctccgatcag cactgcatcg gttggtcttg
       61 tggagtaagt ggatgggcga cccctgcgca tcatcgaccg atccatgatg tcgtcatgta
      121 tcaatgcgaa ggtgtggagc atctcgagac tgctggccgc caagatgatc tccggacagt
      181 gttccccgcc gagaccccgg aaggccatgt agcagaatgt gggccttatc cgcttgcctc
      241 ctgatcggac caaccgctcg atctcatcga ccatctcgac gcagggtggg tggatcgatc
      301 tcatgcgatc gcgaaggtcg tcgagcgaga cctggatggt ctcgtcgaac acctcacgaa
      361 gttgcggcag agattggacc gagacccgga tgtcagacca cctcgtcgag ggcagacgac
      421 agttcgtggg cgatcacggg tccctcgaac cgagccttga tgacgccgtc ggagtcgatc
      481 acccacgtcc atggctcgct gtcgagctgc caagccgatg cgggggccga gatcgtcgtg
      541 gccgaatcat cgcgataagg ctcggcatgg atgaagatcg cgcggtctcc gtgcgtttca
      601 cgagtcgcca ggacctcctc gaggaccgga ccgcacaccc gcgacttgca tagtctcggg
      661 gaggccacga tgaagaccac tggcttgcct gacgtgagtg ctgcctcgag gctcgactca
      721 tggcatgggc atccggggat ccgcgtacag atcccctgga ggcccccggg gtcggtggca
      781 acgggagtag gtgtcgagac ggctggctgc ccgatccctg ggacgcggcc ccctgcaaga
      841 gcctgaagcg cagtgaagcc gaccttcttg cgccccgcaa cgaccacctc gactgccaga
      901 tcgactatcc cgggggccac aaggtcgatc gcaccgacat agaaacttgt cggatcgccc
      961 tcctgaggtg acgaggttcg aggggcgaac cgttcgagac cgagatgcag cggtccatgc
     1021 acacgaccgc gactcgaaaa ccagagggat gggtgcagtg attcgatctg cctactctcc
     1081 ctgtcttcta tccgcatggt tatccggttg gtgtcgttgg ctagatactc ctgtcctcct
     1141 aggaacagtt ggagtcccgg ctcgtcgcca gcgaacctat cggcgagggc cgcgaaggcc
     1201 tttgggatgt tttctgggtc gcgtccagag ataccgcatc cagccaatgt cctgatcaga
     1261 gtcaccgaca gcgctccgcc gcccgccaag cgcaggaact cgcgtctatc caaagtgagc
     1321 caactgtatc tgctagcatc cccagccgtg aaacccaaca cgcctgaagt acatccgcgc
     1381 catcgagcct tctggtggct tctgatgttc gccctagtgg ttctcgctgc atgctctcgc
     1441 gacgcgccgc agaacttcct gcacccgatc ggccccatcg ccaaggaaca agatgggctc
     1501 tggcgtctca cattcggcat cgctgccgcc gtcttcgtca tcgtcgaagg cttgctcgtg
     1561 ttttcgatct tccggtttcg ggcacgacct cacggcgccg agcccaagca gacgcatggc
     1621 aacacgaagg cggagatcgc ctggaccatc gctcctgtcc tgctcctgac gttcgtggct
     1681 tttccaaccg tcagcgccat cttcaagctt gcgaaagagc ctaccgatcc tcttcaggtg
     1741 caggtcgtgg cccaccagtg gtggtgggag taccgttatg aaggatccgg cgtcgtgacg
     1801 gccaacgaac tgcacatccc ggttggcagg cccgtcgatc tatcgttgga gtcggccgat
     1861 gtcatccact cgtactgggt ccccaagctc gctgggaaac aggatgtgat ccccggcaga
     1921 gtcaaccatc tgaccatcca ggccgacgaa ccgggcgtct tcgagggcca gtgtgcggag
     1981 tactgcggct tctcacacgc gaacatgcgt ctgcgcgtta ttgcggagac cgagtctgat
     2041 ttcgagtcct ggctccaatc tcagaagcga tccgctaggt cgccgacaga ccccctcgct
     2101 ctagagggcc agcgtctgtt cctggagggt tccttcgcga atggtcagca gtgcagcggg
     2161 tgccatgcca tcaacggaac aacggctgca ggaaccttcg gaccggacct cacccacctc
     2221 gccagccgta ccagcttcgc tggctccatg ttcgagatga cagatgacga tcttgtcgct
     2281 tggcttcgtg accccccggg gcggaagccg ggttcacaga tgcccaacct caaactcacc
     2341 gactcacaga tccaggccct ggtcgcctac ttgaatgccc ttacatgatg ggagagtcct
     2401 gatggcaacc gtcgttgaaa ctccgaggat cctgcgtcgg ccttcgtcca agacggggat
     2461 cgtcggctgg ttgaccactg tcgatcacaa gaagatcggc atcctctacg gttacacggc
     2521 cttcctcttc ttcatacttg gcggggtcga agcgctgctc atacgtctgc agttggccac
     2581 gccagatggg aaggtgctga gcgcagacct ctacaaccag ctgttcacca cccatgggat
     2641 cacgatgatc ttcttggtcg tcatgcctct gggggccgca ttcttcaact acttgatccc
     2701 gctcatgatc ggcgcgcggg atgttg
//
LOCUS       DATDOZ010000240         3075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_199729, whole genome shotgun sequence.
ACCESSION   DATDOZ010000240 DATDOZ010000000
VERSION     DATDOZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199729"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VI541_04860"
     CDS             <1..1600
                     /locus_tag="VI541_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLF70267.1"
                     /translation="PPDDRHGLPSFRRVLDSVHEAWLERGAELTEQGRELMRRIDDQS
                     RPKASTEPLTNSLLSHAVAHITESFDPTYGGFGTAPKFPQAPVLEFLLNAAAHDLGRS
                     REMVSTTLRRMALGGIYDQLGGGFARYSVDPTWLIPHFEKMLYDNAQLARVYLHAWQS
                     TKEPLYKRIAEETLDYMLSDLLLSHGGFAASEDADSEGVEGRFYVWSYEEFADIAPDA
                     VDYYNVRREGNFEGGSILTASSDDPPESRTTLLEHRSKRIRPARDDKALTSWNGLAIG
                     ALAEAGAALRRSDFIAAASRAANFLLERCVIEGRLMHSHRESITKVTGMLEDYAYLCE
                     GLLTLYEVTFDGRWYEAAASLANQMVELFWDEGDGGFFTTGSDHEKLIVRQKELVESA
                     KPCPSAVATAVLQKLAVLQGSPELHRKATEVLRQAHTYMARAPQVSGTFLRALEAYLV
                     PPREIVVMGERDDPSTRALLGEIFSGFLPNKVVAGGPPTIDSPLLEGKTVIDGKPTAY
                     VCRNYTCEAPVTAAEELRSQLTGS"
     gene            complement(1597..2202)
                     /locus_tag="VI541_04865"
     CDS             complement(1597..2202)
                     /locus_tag="VI541_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70268.1"
                     /translation="MDRDLDRKTTEHILSGAADEVEGFEGLIALVSAISTPTSPEASS
                     REVQIVDQIVRAVHAPDRRRQVLKGSMRWKLSAVTVGGLLALMTGLAFAGSLPGAAQD
                     VASEMLSKVGITVPASDHANYHSQTRGKSGDAHGTESDSHGKGFDVTSTVNSTSPGLE
                     RGKAVSDVASDGKSVTGTANAQDHAVTGTANSNEHRPSPAP"
     gene            complement(2235..2786)
                     /locus_tag="VI541_04870"
     CDS             complement(2235..2786)
                     /locus_tag="VI541_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF70269.1"
                     /translation="MDDYEGRFDELLQGLRTGDTWAVTLLYRKLNAPLLRYLSAQESD
                     EADDLASDAWLGLAPMFAAFKGDESDMRALLFTIARRRLLDLRRKRRRHQTYPTAPAS
                     MPDRVAPDDPEAQTLESESTARALDLIGKLPPDQREVILLRVVAGLDVEHTATVIGKR
                     PGTVRVLAHRGLKNLQSMLSDQV"
     gene            complement(2811..2993)
                     /locus_tag="VI541_04875"
                     /pseudo
     CDS             complement(2811..2993)
                     /locus_tag="VI541_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
BASE COUNT          603 a          968 c          910 g          594 t
ORIGIN      
        1 cccaccggac gacaggcacg ggcttccgag tttccgccgg gtgctcgact cggtccacga
       61 agcctggctc gaacgaggcg cggagctgac cgagcagggt cgagaactga tgcgtcgcat
      121 cgatgatcag agtcgcccca aggcatcgac cgaacccctc acgaactcgc ttctcagcca
      181 cgcggtggcg cacatcactg aatccttcga cccgacgtac ggcgggttcg gcacggcccc
      241 caagttcccg caagcccccg tcctcgaatt cctgctcaat gctgcagcac acgacctggg
      301 gcgctcgcga gagatggtct caaccaccct taggcggatg gcactcggtg ggatctacga
      361 tcagctcggc ggcggcttcg cccggtactc ggtcgaccca acttggctca tcccccactt
      421 cgagaagatg ctctacgaca acgctcaact ggcccgcgtc tatctgcatg cttggcagtc
      481 gacgaaggag cccctataca agcggatcgc cgaggagaca ctcgactaca tgctgagcga
      541 cctactgctc tcgcatggcg gtttcgcagc gagcgaggac gcggactccg agggcgtcga
      601 gggccgtttc tatgtctgga gctacgagga gttcgccgac atagcacctg atgcggtcga
      661 ctactacaac gtgagacgtg aaggtaactt cgaaggagga agcatcctca ctgcgtcatc
      721 cgatgaccca cccgaatcga ggacaacgct tctggaacac cgatcgaaac gcataagacc
      781 cgcgcgcgac gacaaggcac tcacctcgtg gaacggcctc gcgataggtg ctctcgccga
      841 agcgggggcc gcgctgcgaa ggtccgactt catcgcagcc gcgtcacggg ccgctaactt
      901 ccttctcgag cgatgcgtca tcgagggccg cctgatgcac tcgcatcgag aaagcatcac
      961 caaggtgacg gggatgctcg aggactatgc gtatctatgc gaaggattgt tgaccctgta
     1021 cgaagtgacc ttcgacggtc gctggtacga agcggccgct tcgctggcca accagatggt
     1081 cgaactcttc tgggatgagg gcgacggcgg attcttcacc acgggttccg accatgagaa
     1141 attgatcgtt cgtcagaagg agttggtcga gagcgcaaaa ccatgtccga gcgctgttgc
     1201 gaccgcggtc ctgcagaagc tggcagtgct ccaaggctca cccgagttgc accggaaagc
     1261 cacggaggtg ctccgtcagg cgcacaccta catggcgcgt gcccctcagg tctccggcac
     1321 tttcttgcgg gccctcgagg cctacttggt accaccgaga gagatcgtgg tgatgggcga
     1381 gcgcgacgat ccatcgacgc gtgcgctact aggggagatc ttctcgggtt tccttccgaa
     1441 caaggtcgtc gccggcggcc cacccactat cgactcaccg ctgcttgagg gtaagaccgt
     1501 catcgatgga aagccgaccg cgtacgtctg ccgcaactac acctgcgaag cccccgtaac
     1561 cgctgccgaa gagttacgtt ctcagctaac tggttcctag ggggccggac tggggcgatg
     1621 ctcgttcgag ttggccgttc ctgtcactgc atggtcctgt gcattcgcag tccccgtaac
     1681 actctttccg tcgctagcga catccgaaac agccttgcct cgttctaggc cgggcgaggt
     1741 gctgttgact gtcgaggtca catcgaagcc cttcccgtgg ctatctgact cagtaccgtg
     1801 cgcgtcgcca gacttcccac gtgtctggga gtggtagttg gcgtgatctg acgctggcac
     1861 ggtgataccg accttcgaga gcatctcgga cgcaacatcc tgggcggccc ccggaaggga
     1921 ccccgcgaac gccagccccg tcatcaaagc aagcagcccg ccaaccgtaa ccgctgagag
     1981 tttccacctc atcgaaccct tcaaaacctg tctccttcgg tcgggggcat gcactgccct
     2041 aactatctga tcgacgatct ggacctcacg cgaagacgcc tcgggcgagg ttggggtaga
     2101 gatcgcgctg accagcgcga tcagtccctc gaatccctcg acctcatccg cagcacccga
     2161 aaggatgtgc tccgtagtct tgcggtccag atccctatcc atcggttagt agatcgcccc
     2221 ggggtaccca ggcgttacac ctggtccgag agcatgctct gaagattctt gagcccccga
     2281 tgggccagca ctctgaccgt gccggggcgc ttgccgatca cggtggccgt atgttccaca
     2341 tcaaggccag ccaccactcg aagcaggatg acctcgcgct gatccggcgg cagtttgccg
     2401 atcaggtcca gagcacgcgc ggtcgactct gattcgagcg tctgagcttc cgggtcatcg
     2461 ggtgcgacgc gatcgggcat cgacgcgggg gccgtgggat aggtctggtg tcgtcgccgt
     2521 ttgcgtctca gatcgagcag gcggcgtcga gcgatcgtga acaggagtgc acgcatatcc
     2581 gactcgtccc ccttgaacgc ggcgaacatc ggagcgagac cgagccatgc atccgacgcc
     2641 agatcatcgg cttcatcgga ctcctgtgcg gagaggtaac ggagcagggg cgcgttgagc
     2701 tttcgatata ggagggttac cgcccaggta tctcccgtac ggaggccctg gagcagttcg
     2761 tcgaaacgac cttcatagtc atccacctgt gcattatcgg caggaaagac tcatgcaggg
     2821 atcttggcga ggatgtcctt gttctttggg aactcgccgg ttttcttttt cgaccagatc
     2881 tcggtgccat ctagcaggat ctcgaaacgg cccccgtccg aggggatgag agtgagctcc
     2941 gagatctgac tcgatctctc ggcgagtagt tcagccgcca gaccgacggc cctatctgtg
     3001 tagtttcaaa ctacgcagta ttcgatactc agcttcatgg gtccccctag agtgattcca
     3061 cgacataccc ggcct
//
LOCUS       DATDOZ010000241         3744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_995624, whole genome shotgun sequence.
ACCESSION   DATDOZ010000241 DATDOZ010000000
VERSION     DATDOZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995624"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2299)
                     /locus_tag="VI541_04880"
     CDS             complement(<1..2299)
                     /locus_tag="VI541_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HLF70270.1"
                     /translation="MPDPEKIRLSEARSDKAPWKRWGPYLSERAWGTVREDYSPDGNA
                     WEYFPFDHARSRTYRWNEDGLAGICDDHQILCFALSMWNGKDPFLKERIFGLTNPQGN
                     HGEDPKDYWFYVDSTPTHSWMRWRYFYPQEEFPYQDLLDTNRSRSKDDPEYELVDTGI
                     FNDSRYWEITADYAKASAEDICIVIRVRNLGPESATIELLPTLWFRNTWSWGIDNYRP
                     KVSLIDGEVVADHNALGLRTLYCEGKPETLFCENESNAKHLSSGTNATAYPKDGINDY
                     VVSGKPTVNPENTGTKVALRYRMTVGPGETEEINLRLSDGPSDMGDDFHSVMATRRAE
                     ADAFYKLLTPKGTTQDEATVMRQAFAGMLWTKQFYHYDVERWLKGDPAGPPPPESRRQ
                     GRNNHWQHLDNYDVISMPDKWEYPWYAAWDLAFHCVPLAHVDPDFAKNQLLLVLREWY
                     QHPNGQIPAYEWNFGDVNPPVHGWAALKVFEIDGGKDRDFLERVFHKLLLNFTWWVNK
                     KDREGNNLFEGGFLGLDNIGPIDRSSSIQGAHLEQSDGTSWMAMYALNLMKMAVILAQ
                     RDKVYEDIASKFFEHFCLIAHGINSAALASGLWDEDEGFYYDLLHCDNGDKVPLKTRS
                     MVGLIVLYAVEVLEDADVAKLPGFVDRKTWFLSDKSDLLQAIPHIESTGDRNNNLLSI
                     VGPQRLRRILQLMLDETQFLSPYGIRALSAHHLEQPLMIDVAGLKAEVGYEPGESRSG
                     MFGGNSNWRGPIWMPVNYLLIESLKT"
     gene            2345..3331
                     /locus_tag="VI541_04885"
     CDS             2345..3331
                     /locus_tag="VI541_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF70271.1"
                     /translation="MALTEIAQDVFRIESIFGGRLLYLYIYKGSTASLLIDSGASVTP
                     EEAILPELAKLGVEPDMLLVTHCDLDHQGGNHRLKAAFPEMLVACGAGDEALVADPSV
                     LVEQRYSAFEQDHGVGFPTDVKPRLVYLAGGSPVPVDRMFSGGEHLRLSDDWSVEVIH
                     LAGHSRGHLGIFDPRSNSAVTQDAVHGRDYPFADGKPWALMPTYYFVESYLETVEKLA
                     TMKLASLHRAHWPEAKGDAVAKVLAETRDYTLRADALVYELVAQGNSTLREIMETALP
                     VLGEWDVSKAGDFACSMFGHLQRLSDAGLISSERREGLLHYKVTLDYRPPEA"
     gene            3328..>3744
                     /locus_tag="VI541_04890"
     CDS             3328..>3744
                     /locus_tag="VI541_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HLF70272.1"
                     /translation="MRPGAQPPPLGAHPVGDGVSFSVFSRNASAVDVCLFDESGAESR
                     LRAERVESDVWHVVVKGATLGTPYGFRAEGPMDAAGGHRFDASKLLADPYARRIDGEV
                     LWDDAILMPKVDSAPFVPRCVVEDGSFDWANESQPRT"
BASE COUNT          688 a         1066 c         1165 g          825 t
ORIGIN      
        1 aggtcttgag tgactcgatg agaaggtagt tgaccggcat ccatatcggt ccgcgccagt
       61 tggagttgcc cccgaacatg cctgagcgtg attcgccggg ctcatacccg acctcagcct
      121 ttagcccagc tacatcgatc atgagcggct gctcgaggtg gtgcgctgaa agcgcgcgta
      181 tcccgtaagg ggagaggaac tgtgtctcat cgagcatcag ttgaaggatc cttcggagcc
      241 gttgaggtcc cacgatcgag agcaggttgt tgttgcgatc gccggtcgat tcgatgtgcg
      301 gtatggcttg gagaaggtcg gacttgtcac tgaggaacca tgtcttgcga tccacgaatc
      361 ccggcagttt ggcgacatcc gcatcttcga gcacttcgac ggcgtacagg acgatgagtc
      421 caaccatcga gcgggtcttc agggggacct tgtctccgtt gtcacagtga aggaggtcgt
      481 agtagaaacc ttcgtcctcg tcccacagcc cactcgcgag ggcggcgctg ttgatgccgt
      541 gtgcgatcag gcagaagtgc tcgaagaact tgctggcgat gtcctcgtag accttatcgc
      601 gttgagccag gatgaccgcc atcttcatca ggttgagggc gtacatcgcc atccatgagg
      661 tgccgtcgga ctgctcgagg tgggccccct gtatcgagga cgatcgatct atcggcccga
      721 tgttgtcgag tccaaggaag cctccctcga agaggttgtt gccctcacga tccttcttgt
      781 tcacccacca ggtgaagttg agcaacaact tgtggaagac gcgctcgagg aagtcgcggt
      841 cctttccgcc atcgatctcg aacaccttga gtgccgccca gccgtggacg ggcgggttga
      901 cgtcgccgaa gttccactcg taggccggga tctgcccatt ggggtgctgg taccactcgc
      961 gcagaaccag cagtagttgg ttcttggcga agtccgggtc gacgtgggcc agggggacgc
     1021 agtgaaaggc aaggtcccac gcggcgtacc acgggtactc ccacttgtcg ggcatcgata
     1081 tgacgtcgta gttgtcgaga tgttgccagt gattgttgcg accctgcctg cgtgattcgg
     1141 ggggtggggg cccggcgggg tcgcctttga gccagcgttc gacatcgtag tggtagaact
     1201 gcttggtcca gagcatgccc gcgaacgctt gtctcatcac ggtggcttcg tcctgcgtcg
     1261 ttcctttcgg tgtgaggagt ttgtagaacg catcggcttc cgcgcgacgc gtggccatca
     1321 cagagtgaaa gtcgtcgccc atgtcggacg gaccatctga gagtcgcagg ttgatctctt
     1381 cggtctcacc ggggccaacc gtcatccgat atcgaagcgc caccttggtc ccggtattct
     1441 cagggttaac tgtcggcttg cccgaaacga catagtcgtt gatcccatct ttcgggtaag
     1501 cagttgcgtt ggtgcctgaa cttagatgtt tcgcattcga ctcgttctcg cagaagagcg
     1561 tctcgggctt gccctcgcaa tagagcgttc ggaggccgag tgcgttgtgg tctgctacga
     1621 cctctccatc tataaggctg accttgggtc ggtagttatc gatcccccat gaccatgtgt
     1681 ttctgaacca aagcgttggc agaagctcga tcgtcgcact ctccggcccc aggttgcgga
     1741 ccctgatcac gatgcatata tcttcggccg atgccttagc gtagtcggcg gttatctccc
     1801 agtagcgaga gtcgttgaag atgccggtgt cgaccagttc gtattcaggg tcgtccttcg
     1861 agcgcgatcg gttggtgtcg agaaggtcct gatacgggaa ctcttcttga gggtagaagt
     1921 atctccatct catccaggag tgggtcggag tggagtccac gtagaaccag taatccttag
     1981 gatcttcccc gtgattcccc tgggggttcg tcaggccgaa gatccgttcc ttgaggaagg
     2041 gatccttgcc gttccacatc gacaacgcga agcagaggat ctgatggtcg tcacatatcc
     2101 ccgcaaggcc gtcctcgttc cagcgatagg tacgagatct cgcatggtcg aaggggaagt
     2161 attcccaagc gttcccgtcg gggctgtagt cctctcgaac ggtgccccat gccctctcac
     2221 tgaggtaggg cccccatcgt ttccatggag ccttgtcgct cctcgcctcg cttagtcgga
     2281 tcttctcagg gtctggcatg gaacggaacc ataaacgacg gggatacgtc atacaatccc
     2341 cggcatggca ctgaccgaga tagcgcaaga cgtgtttcgg atcgagtcca tcttcggagg
     2401 tcgacttctc tacctgtaca tctacaaggg aagcaccgcg agtctgctca tcgattcggg
     2461 ggcctcggtg acgccggaag aggcgatcct tcccgaactc gcgaagctcg gtgtcgaacc
     2521 ggacatgctg ctcgtgacgc actgcgacct ggaccatcag ggcggtaacc acagattgaa
     2581 ggcggccttc cccgagatgc tggtcgcatg cggcgcaggt gacgaggctc tcgtggcgga
     2641 tccatcggtg ttggtcgagc aacgatactc agccttcgag caagatcacg gcgttgggtt
     2701 cccgaccgac gtgaaaccgc gtctcgtcta cttagctgga gggtcccccg tccctgtcga
     2761 tcgcatgttc agcggtggcg aacatctacg gctctcggat gattggtcgg ttgaagtgat
     2821 ccatctcgcg ggacactccc gcgggcacct tgggatcttt gacccgcgct cgaacagcgc
     2881 ggtgactcaa gacgcggtgc acggacgcga ctacccattc gccgacggca agccgtgggc
     2941 gctgatgccc acctactact tcgtcgagtc ctatctggaa acggttgaga agctggccac
     3001 gatgaagctc gcctcacttc atcgggcaca ttggccggag gccaagggag atgcggtggc
     3061 caaggtcctt gccgaaacgc gtgactacac cttgagggcc gatgcccttg tctatgaact
     3121 ggttgcgcag ggcaactcga ccttgcgcga gatcatggag acggccctgc ctgtgctcgg
     3181 ggagtgggat gtatcgaagg ccggagactt cgcgtgttcg atgttcggtc acctgcaacg
     3241 actgtctgac gctgggctca tctcgtcaga gaggcgtgag ggcctgctgc attacaaggt
     3301 gaccctcgac tatcggcccc cggaggcttg aggccaggcg cccagccgcc gcctctcgga
     3361 gcgcatcccg tgggcgacgg agtctccttc tcagtctttt cgagaaacgc ttcggccgtg
     3421 gatgtctgcc tcttcgatga gtcgggggcc gaatctcgac taagggccga gcgtgtcgag
     3481 agtgatgtct ggcacgtcgt ggtgaagggc gcgaccttgg ggactccata tggctttcgg
     3541 gctgaagggc ctatggatgc ggcagggggt catcgtttcg acgcgtcgaa actgcttgcg
     3601 gacccatatg cgcggcgaat cgacggagag gtgctctggg acgacgccat cctgatgccc
     3661 aaggtcgact cggccccctt cgtcccccgc tgcgtcgttg aggatggatc cttcgactgg
     3721 gcgaatgagt cgcaaccgcg aacc
//
LOCUS       DATDOZ010000242         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_203220, whole genome shotgun sequence.
ACCESSION   DATDOZ010000242 DATDOZ010000000
VERSION     DATDOZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203220"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VI541_04895"
     CDS             complement(<1..349)
                     /locus_tag="VI541_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLF70273.1"
                     /translation="MADTQHTNRLIDETSPYLLQHAHNPVDWYPWGPEAFARAGAESK
                     PVMLSIGYAACHWCHVMERESFEDIGTAEMMNDRFICIKVDREERPDIDAIYMDAVQA
                     MTGHGGWPMTMFLA"
     gene            complement(352..1164)
                     /locus_tag="VI541_04900"
     CDS             complement(352..1164)
                     /locus_tag="VI541_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLF70274.1"
                     /translation="MKMNTRQLDVDGLKVDVYEPDGPSKARLLFSHGAWVGGWIWETF
                     APWFAENGYTAFVPTWRGRYDSRPNIDLGKVSVLDFVDDCLSVAAATAPDVLIGESAG
                     GLIAQKAAESLKDLKALVLMNSAPPFMVPASPAVTIRQIKYFGDILLGRPNKPSFPDY
                     KALILNNVGDKESHEMYARICLESGTALREMSMGRIKVDPNKINCPVCVVVGHLDTIL
                     PLKVHRKIARLYGADLLEYPKMSHHTFSEQGWQEVASEVSGWLEKTLEQRVP"
     gene            1216..2349
                     /locus_tag="VI541_04905"
     CDS             1216..2349
                     /locus_tag="VI541_04905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLF70275.1"
                     /translation="MRVESPDMTLEIGITANPIPPVELTVASAVSAEADGYDSIWYPD
                     HLMGWFPKSLWTVENSSIVNLLPSPHLYLDPAIVLALVAQSTTRLRVATGVTDPIRRP
                     PAELARTFVTLSLVSQGRAIMGIGAGEKENTEPYGLDFRKQVSRLEEALHIVRLLWGA
                     DGPVSFDGRFWKLDRAIFELKPYEGVDPPIWVGAHGPRMLGITGRYGDGWYPSYPMTP
                     SEYAEKLALVRKAATDAGRDPMSIRAGYQMYVVVGEDHETTHRIMSSPLAGAMALVAP
                     AAQWETAGFQHPFGSGFEGLRDYVPEWYSEQELKDATSQFSIEVIHPTIVHGTVDEVV
                     DQVAAFVEVGLEHVAFTNMAPLAGFEYLPATQAGLKEVAARLR"
BASE COUNT          464 a          758 c          750 g          505 t
ORIGIN      
        1 gagcgaggaa catcgtcatg ggccaccccc catgccccgt catcgcctga acagcatcca
       61 tatatatggc gtcgatgtca gggcgctcct ctcgatcgac cttgatgcag atgaaccggt
      121 cgttcatcat ctcagccgtg ccgatatcct cgaaagactc gcgctccatc acatggcacc
      181 agtggcaggc cgcataaccg atcgacaaca tgaccggctt gctctcggcc cccgctcgcg
      241 cgaaagcttc aggcccccat gggtaccagt cgactgggtt gtgcgcatgt tgcaggaggt
      301 atgggctggt ctcgtcgatg agtctgttgg tgtgttgcgt gtcggccacc gtcaaggcac
      361 tcgctgttca agggtctttt cgagccagcc ggagacctcg gaggccactt cctgccagcc
      421 ctgctctgag aacgtgtgat gcgacatctt cgggtattca agaaggtctg ctccgtagag
      481 tcgagcgatc tttcgatgaa ccttcagtgg aaggatcgtg tcgaggtgcc ctaccaccac
      541 gcagaccggg cagttgatct tgttcggatc gaccttgatc ctgcccatcg acatctcgcg
      601 aagcgcggtc cccgattcga ggcagatgcg cgcgtacatc tcatgcgact ccttgtcgcc
      661 gacgttattc aggatcagtg ccttgtaatc ggggaaagag ggtttgttcg gtcgtcccaa
      721 gaggatgtcg ccgaagtact tgatctgacg gatggtcacc gcgggactgg ccgggaccat
      781 gaatgggggg gccgagttca ttagcaccaa ggccttcagg tccttcaacg actcggcggc
      841 cttctgcgcg atcagcccgc ctgcggattc accgatgaga acgtccggtg cagtggcggc
      901 agcaaccgag aggcagtcgt cgacgaagtc gaggacggac accttcccga ggtcgatgtt
      961 tggccgcgag tcgtagcgac cacgccatgt cggaacgaac gcggtgtatc cgttctcggc
     1021 gaaccagggt gcgaaggtct cccagatcca cccgccgacc catgcgccgt gcgagaagag
     1081 cagccgtgct ttcgagggtc cgtccggctc atagacgtcg accttcaggc cgtcgacatc
     1141 gagttgacga gtgttcatct tcattcccct tccttgttga ggctccatcg gaaccagcgg
     1201 tcagcgtatc ccgaaatgcg cgtagaatcc cccgacatga ccctcgagat cgggatcacc
     1261 gccaacccca tcccccctgt cgaattgacg gttgctagcg ctgtttctgc tgaggctgac
     1321 ggatacgact cgatctggta ccccgaccat ctgatgggtt ggttcccaaa gagtctttgg
     1381 acggtcgaga actcttcgat cgtgaatctg ttgccgagcc cgcacctcta tctcgacccc
     1441 gcgatcgtcc tcgccctcgt ggcgcagtca accaccagat tgcgtgtcgc gaccggtgtt
     1501 accgacccga tccgccgtcc gcccgcagag ctcgcaagga ccttcgtaac cctctctctc
     1561 gtctcgcagg gcagagcgat catggggatc ggcgctggcg agaaggagaa caccgagcca
     1621 tacgggttgg actttcgcaa gcaggtgtcg cgactcgaag aagctctaca catcgtccgt
     1681 ttgctatggg gggccgacgg tccagtgagt ttcgacgggc gcttctggaa actggatcgc
     1741 gctatcttcg agttgaagcc gtacgagggt gtcgacccac cgatctgggt cggagcccat
     1801 ggcccccgga tgcttgggat caccggccgt tacggggatg gctggtatcc ctcctatccg
     1861 atgactccct cggaatatgc agagaaactc gcactcgttc ggaaggcggc gaccgatgct
     1921 ggcagggacc cgatgtcgat acgggctggc tatcagatgt atgtcgtcgt cggtgaggat
     1981 catgagacaa cgcatcggat catgtcctcg ccccttgctg gtgccatggc ccttgtagcc
     2041 cccgctgcgc agtgggaaac cgcggggttc cagcatccat tcggatcggg attcgagggc
     2101 cttcgcgact acgttcccga atggtattca gagcaggagc tcaaggacgc cacctcgcag
     2161 ttcagcatcg aagtgatcca ccccaccatc gttcatggga ccgtcgatga agtcgtcgat
     2221 caggtcgccg cgttcgtgga ggtgggtctc gaacatgttg cattcacgaa catggccccc
     2281 ctcgcggggt tcgagtacct gcccgcgacg caggcgggac tcaaggaggt tgcggcacgc
     2341 ctccgctgac ccttgggcag ctgaccctcc atatccgacg gctgcccgcc cgagaacggg
     2401 gaggagggtg agggtcggta tccatgtgca gggtttcaaa gaaggggccc ccgtgggccc
     2461 agggaacgcc gccgagc
//
LOCUS       DATDOZ010000243         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3591611, whole genome shotgun sequence.
ACCESSION   DATDOZ010000243 DATDOZ010000000
VERSION     DATDOZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3591611"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VI541_04910"
     CDS             <1..465
                     /locus_tag="VI541_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HLF70276.1"
                     /translation="IGDEFEIEKARYHKVILLADADVDGSHIRTLLLTLLFRHMRGLI
                     EAGFVYLAQPPLFRVKVGAKVHYLKDEAALEEFRKEHPKVRATRFKGLSEMDAEELRV
                     TTMDPETRTLLRVELEDALRAEEIFSTLMGDDVAERKAFIQHSAKDIRFLDI"
     gene            472..>2209
                     /locus_tag="VI541_04915"
     CDS             472..>2209
                     /locus_tag="VI541_04915"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HLF70277.1"
                     /translation="MAHRTPPPGDEGLNPIGTIDINAEMEQSFVDYAMSVIVARALPD
                     VRDGLKPVQRRIIYSMFENNIGPTTPHRKCAKVVGEVMGNYHPHGDSAIYDALVRMGQ
                     DFSLRHLLIDPQGNFGTIDDGPGASRYTECRLTQLSMRLLEGIRENTVGFNENYSGES
                     EEPAVLPARFPNLLVNGSQGIAVGMATNIPSHNLAEIIDACLHSLANPDADSEELMRF
                     VKGPDFPTGGYLVGTAGIREALASGRGSVKMRAVADVEEVSKGRTAIVITQLPYQVSI
                     ERVIEKIRVLVDDKKIAGIADARNESDNRRGTRFVIYLKKDAVPQVILNQLYKNTPLQ
                     ESFGVNMVALVDGIPRTLNLADMIGHYLDHQMEVIERRTIFRLHEAEARAHIVEGLII
                     AIDNIDEVVAIIRASTDTPEARSRLMQRFELSEIQSNAILDMPLRRLTALEVDKLREE
                     LASLHALIAELKAILADPARRRAIIKEDLEEIKEKFGEPRRSQIVPDEGEMSLEDLIA
                     DDELIVTVSASGYIKSVPANIYKTQGRGGRGVKAAEVSGEDVVAYLVHTTAHAYLLFF
                     TNKGLVHRAKAHE"
BASE COUNT          481 a          673 c          647 g          408 t
ORIGIN      
        1 atcggtgacg agttcgaaat cgagaaggcc cggtaccaca aggtcatcct cctggccgat
       61 gccgatgtgg acgggtccca catccgcacc ctccttctca ccctgttgtt ccgacacatg
      121 cgaggtctga tcgaagccgg cttcgtctac ctggctcagc cgccgctttt cagagtaaag
      181 gtgggagcaa aggtccacta cctgaaggac gaagcggctt tggaggagtt tcgtaaggaa
      241 catcccaagg tacgagccac ccggttcaag ggtctcagcg agatggacgc agaggagctg
      301 cgggtcacca ccatggaccc cgagacccga accctgctgc gggtggagtt ggaagacgca
      361 ctcagggccg aggagatctt ctccacgctt atgggagatg acgtggccga acgcaaggcc
      421 ttcatccagc acagcgccaa agacatccga ttcctggaca tttgagaact catggcacat
      481 agaacccccc cacccggcga tgagggcttg aacccgatcg ggacaatcga catcaacgcc
      541 gagatggagc agtcctttgt cgactacgcc atgtcggtca tcgttgcccg cgctctaccc
      601 gatgtgcgcg atggtctgaa gccggtgcag cgacgaatca tctattcgat gttcgagaac
      661 aacatcgggc ccaccacccc tcatcgcaag tgcgccaagg tggtcggtga ggtcatgggc
      721 aattaccacc cccacggcga ctcggcgata tatgacgccc tagttcgcat ggggcaggac
      781 ttctcgctgc gacatctgct aatcgatccg cagggaaact ttggaacaat tgacgatggc
      841 cccggcgcct cccgttacac cgagtgccgg ctgacccagc tttccatgcg gctgctcgaa
      901 ggcattcgcg agaacaccgt cggttttaac gagaactact ccggcgaatc ggaggagccc
      961 gcagttcttc ctgctcgttt ccccaacctc ctcgtcaacg gcagccaggg gatcgccgtc
     1021 ggcatggcta ccaatatccc atcgcacaac cttgctgaga tcatcgatgc ttgcctccac
     1081 agcctcgcga atcctgacgc cgattccgag gagctgatgc ggttcgtgaa gggtcccgac
     1141 tttccgaccg gtgggtacct ggtgggaacc gccggcatcc gcgaggctct tgcttcagga
     1201 cggggctcgg tcaagatgcg ggccgtagcc gatgtcgagg aggtcagcaa ggggcggacc
     1261 gccattgtca tcacccaact gccgtatcaa gtttccatcg agcgtgtgat cgaaaagatc
     1321 cgggtgttgg tcgatgacaa gaagatcgcc ggcatcgccg acgctcgcaa cgagtccgac
     1381 aaccgccgcg gtacgcgatt cgtcatttac ctgaagaagg acgccgtacc tcaggtgatc
     1441 ctcaatcagc tctacaaaaa caccccgctc caggagagct tcggggtgaa catggtggcc
     1501 ttggtagacg gcataccgcg caccttgaac ctggccgaca tgatcggcca ctacctcgac
     1561 caccagatgg aggtcatcga gcggcggacc atattccgtc tacatgaggc ggaggcccgc
     1621 gcacacatcg tcgagggctt gatcatcgcc atcgacaaca tcgacgaggt ggtggcgatc
     1681 atccgggctt ctaccgacac ccccgaggcg cgcagtcgct tgatgcaacg cttcgagctg
     1741 tcagagatcc agagcaatgc cattctcgac atgccattgc ggaggctcac ggccctcgag
     1801 gtcgacaagc tgcgggagga gttggcctcg ttgcatgccc tgatcgccga gctcaaggcg
     1861 atcctggccg acccggcccg ccggcgagca atcatcaaag aggacctcga agagatcaaa
     1921 gagaagtttg gggagccgag acgcagtcag atcgtgcccg atgaaggcga gatgagcctt
     1981 gaggatctca tcgcagacga cgagctgatc gtcaccgttt ccgcatcggg ctacatcaag
     2041 tcggttccgg ccaatatcta caagacccag ggccgcgggg gacggggggt caaggcggcc
     2101 gaagtatcag gggaagatgt ggttgcgtat ctggttcata ccacggcgca cgcctatctc
     2161 cttttcttca ccaacaaggg tctggttcat cgggccaagg cccacgaga
//
LOCUS       DATDOZ010000244         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_722953, whole genome shotgun sequence.
ACCESSION   DATDOZ010000244 DATDOZ010000000
VERSION     DATDOZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722953"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VI541_04920"
     CDS             <1..376
                     /locus_tag="VI541_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70278.1"
                     /translation="VASSGGKLLVAGGRTPTDTASAEEFDPATGRWSTIASMSSPRGG
                     IAGAATANGLVVIVGGEAQTVFDKVEGYDVKAGRWSLLPPMLTARHGLGVVSIGNKIY
                     TVAGGHTPGYAFTDANEVLDLS"
     gene            483..1934
                     /locus_tag="VI541_04925"
     CDS             483..1934
                     /locus_tag="VI541_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HLF70279.1"
                     /translation="MFGIDESAASPPLEGDGRDDLAALPEEALEDELTLLTGQISAAL
                     GRWHTLLGEFDRRRVWAARGCRSPHHYLSWKLGVDRASSIENLRVAEALQALPLVQSA
                     FCAGTLSYSKVRRLTRIATPETEAELVEIATHTTASQMGRLASAFRGALAAMADPVEK
                     HRNRHLNWYEDDDGSVVISGRLSPEDGAVVVKALDSFEKALRKSRPHPIRIERSAAGA
                     IEVIPDRPAIAAIEKDAHEPAASVRADALLALAEAALASEKVGRVGGDRHLVVVHVDV
                     ETLKGESPTDGDHEGGICEIECGPSISPETARRLTCDASIHPLLMQQGRPIGIGRRSR
                     SVPYWIRVPLHDRDGGCAFPGCGESRYIQIHHILHWVNLGKTELENLVELCWFHHRLM
                     HEGGYSLTTLEDGRLEFRDPSGVLQGLPPPRASEPPPLSEALGELGVEVGVQTCTSNW
                     RGERMDVGYIVGGLMRTPREDHPRPASSADPTG"
     gene            complement(1969..2448)
                     /locus_tag="VI541_04930"
     CDS             complement(1969..2448)
                     /locus_tag="VI541_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF70280.1"
                     /translation="MSHLGEELARRYRHATDDVLSEIAVMTSERWETIVPGEGWAVGV
                     IAHHLAVSCQFVMALLEQVVQGGDIHWTQEFIDDANAMHAEVFEAPDRAETVELLTTQ
                     VDAACRYISSLTQDDLDRRPTTPLDYSGGYVDNSRKIVELMLINHTVSHLESVRSTG"
     gene            complement(2800..3192)
                     /locus_tag="VI541_04935"
     CDS             complement(2800..3192)
                     /locus_tag="VI541_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HLF70281.1"
                     /translation="MTTDTLRDLASGTNFAVISTLMPDGRPQTQVMWVDYEEGRILLN
                     TEVHRQKYRNLQRDPRVTVTIWAMANPYSYVEVRGVVEEFIRGPAALEHIDRLALKYT
                     GSPFRREGITSERVILRIKPDKQVTYGR"
BASE COUNT          681 a         1050 c         1070 g          660 t
ORIGIN      
        1 agttgcatcg tcgggcggga agctcctcgt ggccgggggc cgaacaccaa ctgacacagc
       61 aagcgccgaa gagttcgacc ccgcgactgg tcgttggtca accatcgctt cgatgtcatc
      121 gcccaggggg gggatcgcgg gtgccgccac cgccaacggt ttggtagtga tagtgggcgg
      181 agaagcccag acggtcttcg ataaggtcga gggctatgac gtcaaggctg gacgctggtc
      241 gctcctgccg ccgatgctaa cggcgcgaca tggccttggg gtcgtgagca tcggcaacaa
      301 gatctacacg gttgcgggtg ggcacacccc cggctacgcc ttcacagacg cgaacgaagt
      361 cctagacctc tcctgatcgc gtcgggcccc atgttctgtg acttaactta gttctgtatt
      421 tttttccgaa gagtgtccga acatggcccg ttttgtcaga ggggctctct atcatcgaac
      481 gtatgtttgg gatagacgag tcagcagcat caccccctct cgagggcgac ggtcgggatg
      541 acctagccgc tctccccgag gaggccctcg aggacgagct cacgctcctc accggccaga
      601 tctcggcggc tctcgggcgg tggcacacac ttctaggtga gttcgatcgc cgtcgggtct
      661 gggcggcccg ggggtgccgg agcccccacc actacctctc ctggaagttg ggggtcgacc
      721 gggcatcctc gatcgagaac ctgagggtag ccgaggctct ccaggccctg cctctcgtcc
      781 agagcgcctt ctgcgctggg accctctcct actcgaaggt acgacggctc acacgcatcg
      841 ccacgcccga gaccgaggcc gagttggtcg agatcgccac gcatacgacc gcctctcaga
      901 tgggtcggct cgcctcagcc ttccgggggg ccctggctgc catggcggat ccggtagaga
      961 agcaccgcaa ccgccacctc aactggtacg aggacgacga cggctccgtg gttatctcgg
     1021 ggaggctctc ccccgaagac ggtgcggtgg tggtcaaggc cctcgactcc ttcgagaaag
     1081 ctctgcgcaa gtcgaggccc cacccgatcc gcatcgagcg cagcgctgct ggggctatcg
     1141 aggtgatccc agaccgcccg gctatcgctg cgatcgagaa ggatgcgcat gaaccggctg
     1201 ccagcgtccg ggccgatgcg ctcctggccc tcgcagaggc cgccctcgca tcagagaagg
     1261 tgggtcgcgt agggggcgac cgtcacctcg tggtcgtcca tgtcgacgtg gagaccctga
     1321 agggtgagtc acctaccgat ggcgaccacg aaggtggaat ctgcgagatc gagtgcggac
     1381 catcgatatc accagagaca gcaagaaggc tcacctgcga cgccagcatc caccccctcc
     1441 tgatgcagca gggtcggccc atcggcatcg ggcgaaggtc acgcagtgtc ccctactgga
     1501 taagggttcc cctgcatgat cgagacggtg ggtgcgcctt cccggggtgc ggtgagtctc
     1561 ggtacatcca gatccaccac atacttcact gggtgaatct cggaaagacc gagttagaga
     1621 atctcgtcga gctctgctgg ttccaccatc ggctcatgca tgagggtggg tactcgctaa
     1681 caaccctcga agacggacgg ttggagtttc gggatccttc gggggtcctg caggggctcc
     1741 ccccgccgag ggcctcggag cctccgcctc tctcagaggc tttgggtgaa ctcggagtcg
     1801 aagtgggggt gcagacctgt acttcgaact ggcgaggcga gcggatggat gtgggttaca
     1861 tcgtcggggg cctgatgagg accccgcgtg aagaccatcc gagaccagcc agctccgccg
     1921 acccgaccgg ctaggtcggg ggctcaggca cgcccgcgat caaacgagtc aacccgtcga
     1981 ccggacactt tcaagatgtg agacggtgtg gttgatcagc atcaactcca cgatcttcct
     2041 agagttgtcc acgtagccgc ccgagtagtc gagtggggtt gtgggccgtc tgtcgaggtc
     2101 gtcttgggtc agtgacgaga tgtagcgaca tgcggcatcg acttgagtag ttagcagctc
     2161 gacggtctca gcccgatccg gcgcctcgaa gacctcggca tgcatggcgt tggcatcatc
     2221 gatgaactcc tgagtccagt ggatatcccc cccttggacc acctgctcaa gcagagccat
     2281 gacgaactga caagagacag cgagatggtg tgcgatgaca cccacggccc aaccctctcc
     2341 cggcacgatg gtctcccatc gctcggacgt catcacggcg atctcgctca gaacatcgtc
     2401 agttgcatgc cgataccgcc gcgcaagttc ctccccaaga tggctcatgc caccatcatc
     2461 tatcagttct gtgagggggc gtcatcgaat cgaagggatc tgaaaaggat ggagggggcc
     2521 gaagccccct ccatctaaag atcactgagt tcgagcctac ggcccggact gatggacaag
     2581 gtcagtcctc aggatcccgg aacgctgttt ggattccacc ggcaaccact cggtctagga
     2641 gttgcctgtt cgggaggtga tcctcaccga acgccgagtg gctccctagc agccaggcac
     2701 ctcgcaggtc cagttgggtc tatgcaaaac tggatcacct cccctcttgc gtgataacag
     2761 tttcgcatgg gctgcaagta actcaaagca actgacgtac taccggccgt atgtgacctg
     2821 cttatcgggt ttgatccgaa ggatcacgcg ctcgctagtg atgccctcgc gacggaaggg
     2881 cgaacctgtg tatttgaggg ccaggcgatc gatgtgttcg agggcggctg gcccacggat
     2941 gaactcctcc acgaccccgc ggacctcgac atacgagtat gggttcgcca tcgcccagat
     3001 cgtgacggtc acgcgaggat ctcgctgaag attccggtat ttctgtctat ggacctcggt
     3061 attgagcaat atgcgacctt cttcataatc gacccacatg acctgagtct ggggcctgcc
     3121 atccggcatg agtgtgctga tgacggcgaa attggtacct gaagcaaggt cgcggagggt
     3181 gtcagtagtc atctgccgag actaccaggc ccccaacggg tcgccagaaa ggtttgcgcc
     3241 tcaatggaga agggttttgg gggttaaggt cgaatcccta tagctcaagc caccagggag
     3301 ccaagagatg aaaaagacga agtcactgct cgtactgatc acggccgtag cgatgtcggc
     3361 accgatggct caggcggcta acccctcgtt ctcagaccac tgtgcgactg cctacgcgaa
     3421 catcgctgcg acgctcagct cggatggttc ggtgatgaag a
//
LOCUS       DATDOZ010000245         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_456804, whole genome shotgun sequence.
ACCESSION   DATDOZ010000245 DATDOZ010000000
VERSION     DATDOZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456804"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..879
                     /locus_tag="VI541_04940"
     CDS             175..879
                     /locus_tag="VI541_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLF70282.1"
                     /translation="MRRVFALARQDLLSEARSKEIAPAMVLFALALVFLFTFALPPGA
                     GRAPVPPPVAGAVAVRDMAGTFLWIAILFAAIIGFGRSAAEEKEDSRIDGLLLAPVDP
                     AALFLGKLLANFTFLVILEAAALPFFIVFLDIDPTLLLPGVLPILLLANLGLADIGTL
                     FGAASQHARARALILPLLSFPILLPIVLGASRLTSDLMLTGGFATEGRWFILLTVFDL
                     VTLAIGMVTFEFVIQE"
     gene            876..1553
                     /gene="ccsA"
                     /locus_tag="VI541_04945"
     CDS             876..1553
                     /gene="ccsA"
                     /locus_tag="VI541_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLF70283.1"
                     /translation="MRASALRTLVAITTIAMVAGLVAVFFVVPTDDFQGVVQRVFYIH
                     VPSAWISYLAYTIVLIASIAYLKTGSRQWDALAHSSAEVGVVFTGLTLVTGMLWGKPI
                     WGTYWAWDARLTLTFVLFLIYLGYLAFRWMATEPAGAGRVAAVIGITGFVTVPLVHFS
                     VEWFKGQHPAPIVVDPQGGPHLPVAMLVTFLYMFGVFTLLFTAILAIRYRLARTQLLL
                     DDLEVAS"
     gene            1550..1696
                     /locus_tag="VI541_04950"
     CDS             1550..1696
                     /locus_tag="VI541_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HLF70284.1"
                     /translation="MSGLGYLGLAYAVLWLGLGGYLWSLARRQRSLEKRIDEVIEHQR
                     TPPT"
     gene            1888..2232
                     /locus_tag="VI541_04955"
     CDS             1888..2232
                     /locus_tag="VI541_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70285.1"
                     /translation="MNKSAAKELTWTMAILKALAITAFLLIFLGFVPSIFRYWWAGNS
                     DNISQFLADTVGIRFKEPYTLVRLHDAISMGYQTVAFAIPVAATYFILERRRRRLGQR
                     GAEPVKGYLPGK"
     gene            2229..>2561
                     /locus_tag="VI541_04960"
     CDS             2229..>2561
                     /locus_tag="VI541_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLF70286.1"
                     /translation="MSAIAPRDPFSAENDIEKPRLRTDYIAAEVEPDWLKSIRKPPRY
                     IDFLPELCILCEGCVDICPWYCIFMLDPSIVAESGAEEVSRDMKPNDILFVIDDDACT
                     RCLLCVERC"
BASE COUNT          434 a          866 c          705 g          556 t
ORIGIN      
        1 gcctcgctac ctattgctcg atgaaccgtt caatgcactg gatggatcga ccgccaaact
       61 catcccatcg gcgatagccg cccccggccg aacgctgctt cttgtcaccc atgacgaagc
      121 cagggcgggc acgatcaccg acgaggtcat ccgcatggat gctgggagag tccagtgaga
      181 agagtcttcg ccctggcccg gcaggatcta ctctcggagg cccgctcgaa agagatcgcc
      241 ccggccatgg tgctcttcgc gcttgccctc gtcttcctct tcaccttcgc actgccgccg
      301 ggcgcggggc gggccccggt gccgccaccg gtcgcgggag ccgttgcagt gcgcgacatg
      361 gcaggaactt tcctttggat agcgatcctg ttcgccgcga tcatcggatt cggcaggtcg
      421 gccgccgaag agaaggagga ctctcgcatc gacggcctgc tcctcgcgcc cgtagaccct
      481 gcagccctct tcctaggaaa gctccttgcg aacttcacct tccttgtgat cctcgaagct
      541 gcggcattgc ccttcttcat agtcttcctc gacatcgacc ccacactcct gctcccaggc
      601 gtcctgccca tcctccttct ggcgaacctg ggtcttgccg acatcgggac cctcttcggc
      661 gccgcatcgc agcatgcgag ggcgcgcgct ttgatacttc cgcttctctc attcccgatc
      721 ctgctgccca tcgtcctcgg cgcctctcga ctcacctccg acctcatgct caccggtggc
      781 ttcgcaacgg agggccgctg gtttatcctg ctgacggtct tcgaccttgt caccctcgcc
      841 atcggaatgg ttaccttcga attcgtgatc caggaatgag agcttcggcc cttcgaaccc
      901 ttgtcgccat cacgaccata gcgatggtcg cggggctcgt tgccgtcttc ttcgtcgtcc
      961 ctacagacga cttccagggt gtggttcaac gtgtctttta catccacgtg ccttctgcat
     1021 ggatctcgta cctggcctac accatcgtcc tcatcgcctc gatcgcgtac ctcaagacag
     1081 gctcgcggca gtgggatgcg ttggctcact cgagcgccga ggtgggcgtg gtcttcaccg
     1141 gactcaccct cgttaccggc atgctctggg gcaaacccat ctggggcacc tactgggcct
     1201 gggatgcgcg tttgaccctc accttcgtcc tctttctcat ctacctcggc tacctggcct
     1261 tcagatggat ggccaccgaa cccgcaggtg cgggccgtgt cgcggccgtc atcgggatca
     1321 cgggattcgt gaccgtgcct ctggtgcact tctccgtcga gtggttcaag ggtcaacacc
     1381 ctgcgccgat cgtcgtcgat ccccaaggcg ggccacacct tcctgtggcc atgctcgtaa
     1441 ccttcctcta catgttcggc gtattcaccc ttctgttcac cgcgatcctg gccatccgat
     1501 atcgcctggc gcggacacag ttgctcctag acgatctgga ggtcgcgagt tgagtggatt
     1561 gggatacctc ggtttggcat acgccgttct ctggctcggt ctcggtggct acctctggag
     1621 cctcgctcgc cgccaacgct ctctcgaaaa gcgcatagac gaagtgatcg agcaccaacg
     1681 cacgccccca acgtaggtgg atcaacccac tcgtcctcgg ccaccacgtt cgccgcagcg
     1741 tcggtgccgg aggagtcttc tggcggctca acgtgacctg cggctcgggg ttcctccacc
     1801 tactcagaaa gttcaccaaa gtgtgaatcg cgcgtcggct ataatccggc cgtccaaaac
     1861 caagcgagga cgtgaggacc cataaacttg aacaagagcg ccgccaagga actcacgtgg
     1921 accatggcca tcctcaaggc cctggcgatc accgcgttcc tgctcatctt cctcgggttc
     1981 gtcccatcga tattcagata ctggtgggct ggcaacagtg acaacatctc gcagttcctg
     2041 gctgacacgg tagggataag gttcaaggag ccttatacgc tggtgcgtct tcacgacgcg
     2101 atcagcatgg gttaccagac ggttgcgttc gcgatccctg tcgcggccac ttatttcatc
     2161 cttgagcgcc gccggcgtcg gcttggacaa cggggggcgg aacctgtcaa gggctacctg
     2221 cccggcaaat gagcgcgatc gcacccaggg atccattcag tgctgagaac gacatcgaga
     2281 agccgcgtct tcgcaccgac tacatcgctg ccgaggttga gccggactgg ctcaaatcga
     2341 tccgcaagcc cccccgatac atcgacttcc tgcctgaact ctgcatcctg tgcgaggggt
     2401 gcgtcgacat ctgcccctgg tactgcatct tcatgctcga cccctcgatc gtggccgagt
     2461 cgggggccga ggaggtctct cgcgacatga agccaaacga catcctgttc gtgatcgacg
     2521 acgatgcctg cacgcgttgc ctcctctgtg tcgagaggtg c
//
LOCUS       DATDOZ010000246         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2830553, whole genome shotgun sequence.
ACCESSION   DATDOZ010000246 DATDOZ010000000
VERSION     DATDOZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830553"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VI541_04965"
     CDS             complement(<1..230)
                     /locus_tag="VI541_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70287.1"
                     /translation="MPPHTWMPRLRLIGITLSFILVSTGAVALIERPRNVSTKRVSAV
                     APLVIDDSLVPAHREPPPPPPPPVPPKPVRKAP"
     gene            complement(271..1377)
                     /locus_tag="VI541_04970"
     CDS             complement(271..1377)
                     /locus_tag="VI541_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70288.1"
                     /translation="MRVCFTHLERSRGNRGPLIASSLLLALGGALSLFGRSPADPTVR
                     IYDAFLGAPLTQRVSATVPSTEQGKAKGSPRESAASGPIPAPIPAGPDISAYRGLGAW
                     VDQFDFGRGWSQPPALVVDELARRGVKTLYLQTGKWNSPNDFIDPTVAQTYLQLAHAQ
                     GIKVVGWYLPGFVDVPTDVARSMAVLNFRSATGDRFDGFAPDIEERRAAGGRDRFNAG
                     VAEYSRQLRAAAGPGVALGAITVDAKNNKRAPNTWEGFPWPQIGQYYDVIMPMAYWSV
                     TKRQAECGNSNDVSSYMREMKSETRALMGVDRPFHAIGGIADCITPTEVEAFVGVSKE
                     LGFIGGSLYDFQTTKANSQAESFWALLSRFNAPS"
     gene            complement(1468..>2266)
                     /locus_tag="VI541_04975"
     CDS             complement(1468..>2266)
                     /locus_tag="VI541_04975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLF70289.1"
                     /translation="KGDMANGKSAKRQRQKEQKQAKIEEQNRIIAARRRRVMTINLTL
                     LAVVVGVIGFLLFNQSAKKKAGSETETAVTLNERCKGTPDAGTSAAVAPPGKLRIDLT
                     KTYKAIVETNKGTMTFTLDAKTSPCTVNSFVYLARKKFFDGTVFHRLVKGFALQGGDP
                     LGNGSGGPGYKVVDKPPAGFKYVKGVVAMAKTGAEADGTSGSQFFIVPGDGASTLPAQ
                     YAVLGRVTGGQDVVAAIDDLPTEDNGQGEKSKPTVEVRIIKVTIKES"
BASE COUNT          431 a          696 c          672 g          467 t
ORIGIN      
        1 ggagccttgc gcaccggctt gggaggtaca ggtggtggcg gtggtggcgg aggctctcgg
       61 tgtgcgggga ccagagagtc gtcgataacc agcggtgcta cggcggatac gcgcttggtc
      121 gaaacgttcc gaggccgctc gatgagagcg accgcaccgg tcgacacaag gatgaacgag
      181 agggtgatcc cgatcagacg aagtcttggc atccaggtat gtgggggcat ggcttaccaa
      241 gtgtacggtc ctgcggatga gtcggttgac ttagctcggg gcgttgaaac gactcagaag
      301 ggcccaaaac gattcggctt gcgagttggc tttggtagtt tggaagtcat agaggcttcc
      361 gccgatgaac cccaactctt tggagacgcc aacgaaggcc tcaacctcag ttggggtgat
      421 gcagtctgct ataccgccta tcgcgtggaa tggacgatcg acacccataa gggcccttgt
      481 ctcgctcttc atctcccgca tgtacgagga cacatcgttt gagttgccgc actccgcctg
      541 tcgcttcgtg acgctccagt aagccatcgg catgatgacg tcgtagtact gaccgatctg
      601 gggccacggg aacccctccc atgtattcgg tgcgcgcttg ttgttcttgg catcgacagt
      661 gatagctccc agagccacgc ccggcccggc agcggctctc aattggcgag agtattcggc
      721 cacgcccgca ttgaagcgat ctcggccccc ggccgcacgc cgttcctcga tatcaggagc
      781 gaatccatcg aagcgatctc ccgtggcact gcggaagttg agaaccgcca tgctgcgagc
      841 aacatcggtt ggaacatcga cgaagcctgg cagataccaa ccgacgacct tgatcccctg
      901 cgcatgggcc agttgcagat aagtctgggc cacggtcgga tcgatgaagt cgttaggcga
      961 gttccacttg ccggtctgca ggtaaagggt cttgactccc cgccgcgcca actcatcgac
     1021 gaccagcgca ggaggctgac tccagccacg accgaaatcg aattgatcca cccaggcccc
     1081 cagaccgcga tacgcggaga tgtcgggccc tgccgggata ggggccggga tcggtccgct
     1141 agcggccgat tcacgcgggc tgcccttcgc cttgccctgt tctgtcgagg gaaccgtcgc
     1201 cgacacgcgc tgggtcagcg gggcccccag gaaagcgtcg tagatcctta ccgtcggatc
     1261 cgcaggcgaa cgaccgaata gggagagtgc tccgccgagg gcgagtagga ggctcgacgc
     1321 gatgaggggg ccccggtttc cccgggacct ttcaaggtgc gtgaaacaga ctctcaacac
     1381 ttatcgcccc cgaatgatga acggacagag cccgcaccag ttacggacct gcgctctaag
     1441 agatcccgat gtgccgccta agacatctca ggattctttg atggttacct ttatgattct
     1501 aacctctacg gtcggcttcg acttctcgcc ctggccgttg tcctcggttg gaagatcatc
     1561 gatcgcagca acaacgtcct gacctcccgt cacccttccg agcaccgcat actgcgcggg
     1621 aagagtcgat gcgccgtcac ccgggacgat gaagaactga ctgcctgagg ttccgtcggc
     1681 ctcggcccca gttttggcca tcgctaccac acccttgacg tacttgaacc ccgccggggg
     1741 cttgtcgacg accttgtagc cgggcccccc ggagccgttg cccagcggat ctccaccctg
     1801 gagagcgaac cccttgacca atcgatggaa gaccgtgccg tcgaagaact tcttcctggc
     1861 gagatagacg aatgagttga ccgtgcatgg cgatgtcttt gcatcaaggg tgaaggtcat
     1921 cgttcccttg ttcgtctcga cgatcgcctt gtaggtcttg gtcaggtcga tccgcaactt
     1981 ccccgggggt gccaccgccg ccgacgtgcc tgcatcgggc gtccccttac agcgctcgtt
     2041 caaggtcacg gcggtttcgg tctcggaacc cgccttcttc ttggcactct gattgaacag
     2101 gaggaaaccg ataacaccca cgacgaccgc gagaagggtg agattgatcg tcatcactct
     2161 gcggcgacgc gccgcgatga tccgattctg ttcttcgatc ttcgcctgtt tctgttcctt
     2221 ctgtcgttga cgcttcgcgc tctttccgtt tgccatatca cccttc
//
LOCUS       DATDOZ010000247        10032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3370989, whole genome shotgun sequence.
ACCESSION   DATDOZ010000247 DATDOZ010000000
VERSION     DATDOZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3370989"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VI541_04980"
     CDS             complement(<1..1583)
                     /locus_tag="VI541_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLF70290.1"
                     /translation="MNRIFKRLFFGRPVSTHEELHHRLSKKVALAVFSSDALSSSAYA
                     TDEILLVLVLAGGLALQYSIGISLAVALVLGVVIASYRQTVRAYPSGGGAYIVTKDGL
                     GTFPGLIAAAAILIDYVLTVSVSVAAGLAAVGAAFPAIHNHRVGASLLIVLFITMLNL
                     RGLKESGTIFAIPTYGFLLSMAVLIGVGVFRVLTNTVAPIPPHAITPEVGLSVLVILK
                     AFSAGCTAMTGVEAISDGVPAFKRPESKNASQTLLTLGLLLGFLFLGITFLARALNVD
                     PIQIEHGKTVTSQIGGQIFGSNSPMFFIIQAFTSLILFLAANTAFADFPRLASILARD
                     RFLPRVLQNRGDKLAFSNGIMVLAAGASVVLIKYGALVHRIIPLYVVGVFTSFTLSQT
                     GMVIHWLKAHKAAGTLGTAEPAHWRRNIAINAFGAFTTFVVLVIIAIAKFTGGAWQVI
                     VLIPAVAWLLYRINGHYRSVERRLKLQPVPRAVAPGKVLILVTRFRGATKALAFVRSL
                     VPDQLKIVAMRTSEVRRLNL"
     gene            1668..2333
                     /locus_tag="VI541_04985"
     CDS             1668..2333
                     /locus_tag="VI541_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HLF70291.1"
                     /translation="MHAVIVGCGRVGAELGQALEARGVSVSIIDSDPKSFEQRLQPGF
                     EGKRLAGIGFDKDILEEAGIEKAEIFVSATRGDNTNIVSARIAKERYGVPHVAALIYD
                     PRRAEIYERLGIRTVATVAWATDQILARIMPELASVEWTVGGGEVVVVGVTVPPSLIG
                     KPIEILREPGKIHVVALTRFGTTQIPALKTIIQEGDLIHLSVVRDALSELDELLTSKG
                     DVH"
     gene            2330..3016
                     /locus_tag="VI541_04990"
     CDS             2330..3016
                     /locus_tag="VI541_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HLF70292.1"
                     /translation="MRVAIAGAGKVGRFIAGDLLRRGHQVTLIDADGELITRHRDDLN
                     CRWVQGDACEPLNLQGAALDTCEVMVAATGDDKVNLVASLLAKQEFGIPRVLARVNHP
                     KNAWLFNETWGVDIPVSATHLLTSLIEEAVTVGDLITLLRLEQGKVFLVECKLGENSP
                     VAGKVLHEVTLPRDSSIVAVVRRGHVIPPRGDTPLMIGDEVMALTIPDVQRDLEQLLT
                     GTRGPEEPGD"
     gene            complement(3021..3380)
                     /locus_tag="VI541_04995"
     CDS             complement(3021..3380)
                     /locus_tag="VI541_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HLF70293.1"
                     /translation="MFKRFGDRLAETPEQHHAQEIRLWCAELDAVEQIAECRPRTRRR
                     VAGVVESIKVKPMEHTSVLEVQIFDGTDRIMGVWFGRQQIPGVDLGQRLILEGTMATF
                     KGEMLQIINPAYEIVES"
     gene            3442..3990
                     /locus_tag="VI541_05000"
     CDS             3442..3990
                     /locus_tag="VI541_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HLF70294.1"
                     /translation="MDRQAALDYVRSRTEKETTLRHLITVEGIMRRLAEHLGEDADRW
                     ALAGLFHDLDMELTHHDLSKHAYLAAEWLREDGIDEDIVNTVLAHAHEQYRTDRMSQG
                     IVHADAVAGFLVACALVRPEKTAGMKVSSVRKKLKEKSFAPGVERPEILNVEEKMQIP
                     LDEFLAIAIEGLQSVAEEAGLS"
     gene            4081..4479
                     /locus_tag="VI541_05005"
     CDS             4081..4479
                     /locus_tag="VI541_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLF70295.1"
                     /translation="MAHPNEELARSEMEAAVQGDFEGMLDYYTDDAILHYPGRNALSG
                     THRGKDGIRKWGRKFGELLGEEGSLARTLHDIVVSDDHAIQLVSVEARRSDGRKARWN
                     VACVMHVRDGKISEVWLNIDDPYAVDELLA"
     gene            4654..5298
                     /locus_tag="VI541_05010"
     CDS             4654..5298
                     /locus_tag="VI541_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLF70296.1"
                     /translation="MRHGETASDSVRGGSLRTSVRQRADEIKPYRCKHLIAVIEDPRD
                     VKNIGTVLRNVNALGVEKAYVVDPSGSLPDDWQEMRDQRSLSKTSVSAIKWSFVKRFD
                     STQACIDHLERNRFVSIVTSPHVKGENNVALGEADYTIFPKLAVWFGNEARGISDLAV
                     QHSEMCVSIPMFGMIESLNLGTTSGIVLHEVTKQRREYQRKYRWRKKRGRRIDA"
     gene            complement(5481..6098)
                     /locus_tag="VI541_05015"
     CDS             complement(5481..6098)
                     /locus_tag="VI541_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLF70297.1"
                     /translation="MGHLLDALKIWIEASIKGPSGYAAIFFLMLLESAAIPVPSEITM
                     PVAGLLAQAGRFNFVLVAMLGTLGNLVGSWIAYGMGRYGGRALLIRYGRFVMVKPHDI
                     ERADAWFERYGGRAVFWSRLVPVVRTFISVPAGVARMPLVRFSLLTFVGCLPWSFALT
                     YVGFALGERWKDLLPYADAITYVIAGGLLLLIAIWFYRRIRAKAR"
     gene            6200..8461
                     /gene="topA"
                     /locus_tag="VI541_05020"
     CDS             6200..8461
                     /gene="topA"
                     /locus_tag="VI541_05020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HLF70298.1"
                     /translation="MPNNLVIVESPAKAKTIEKYLGSDFKVIASVGHVRDLPTKTLAV
                     HVDDDFRLDYTVNEDRTKIISSITKAAKSADAVYLATDYDREGEAIAWHITEACSIPL
                     EKQKRITFTEITKKAVTEAVDHPRSVDPMLVDAQQARRCIDRLVGYPVSQLLWEKIRY
                     GLSAGRVQSVALRMVVEREREISAFIPLEYWTLEALFATDKKETFAAKLVQIGERKIA
                     TQISRDAPEDAEGRIPDAAQAEQILAALDGATYQVEDVRTQEKRRSPQPPFITSTYQQ
                     EASRKLGYGARRAMGIAQGLYERGFITYMRTDSTTMSQEAIGAAESLIRKAFGPQYWA
                     GRYSNHDRKTKGAQEAHECIRPTEMSMPRAQVEAAIRAEAPRDSEAMIKVYDLVWKRA
                     VSSLMTQAVFDAVSADIVTAGPPQKHVFRATGSTIKFDGFMRIYMEGQDDDEDEEGGK
                     LPALTATQPLDLEELRPDQHFTAPLPRYTEASLIKELESRGIGRPSTYASIMATLVEE
                     KRGYTRLDRKRFFATDTGEVVSDYLTRFFGDHFMDYQFTSDLEEHLDEMAEGRLSYRP
                     VVESFYTPLKDRMAKAAEITKEEVTTQAIDERCPECGSPLVIKLGRTGKFVACTNYPT
                     CKFTRPLPGQEQAPPELLDEDCPDCGNKLQKRTGRFGPFVGCSTYPECKYIKRKPITL
                     TGVPCPKCAEAPCPSCKKGTAGELVERDWKRGKFYGCNHYPRCRYGQTQDPRTGDVSA
                     PGSSGEGKVTSAP"
     gene            8458..9090
                     /gene="tmk"
                     /locus_tag="VI541_05025"
     CDS             8458..9090
                     /gene="tmk"
                     /locus_tag="VI541_05025"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLF70299.1"
                     /translation="MTATGLFLVFEGGEGSGKSTQIQRLRTFLEESGHKVVVTREPGG
                     SSVGEELRRLVLQHRDQPISPKTEALIYAADRAQHVEEVIRPALEAGAAVISDRYVDS
                     SIAYQGLARGLGLEQIESLNHWGTGGLLPDLVFLLDLDPAVGLRRSGETDRIESEGVE
                     FHVGVRDAYLQLADRYADRFRVLDAGLPPMELETQIKAIVQSLLERAEAR"
     gene            9087..>10032
                     /locus_tag="VI541_05030"
     CDS             9087..>10032
                     /locus_tag="VI541_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF70300.1"
                     /translation="MNNIWDRCPVEGRTRALLESYSQRSGGSYLFIGPQGAGMSEAAE
                     IFAAALMCPDDDGDCDICRRTIAGVHPDARFFEPEGFTYPVETIREITTSASHSPLEG
                     SSRVFVIAEADRIAERSQNALLKSLEEPNASVTWVLLGRSLDMFLPTILSRCRVVEFP
                     AMSEDAIRDALVAAGEVAPDPIVRAARGDLQRAIALAGEGAASQLTELAKRVVLEPEP
                     SPSWVLSISDAVAAVAKAQREVLEQQQATQLEESDLGKGAGGVRKRIADRHKREVRRA
                     ESEVFMMFLSILGDTFRDLAARGVGAPDDALILHRSELD"
BASE COUNT         2123 a         2959 c         3123 g         1827 t
ORIGIN      
        1 aggttcagac gacgaacctc cgaggttcgc atggcgacga tcttcaactg atccgggacg
       61 agagagcgaa cgaaggcgag ggctttcgtt gcgcctctga aacgcgtaac caggatgagg
      121 actttgcccg gagcgaccgc acgcggcact ggctgaagtt tgagcctgcg ctccacgctt
      181 cgatagtgac cgttgatgcg gtacaggagc caagccaccg cagggatgag gacgatcact
      241 tgccatgccc ctccggtgaa tttcgcgata gcgatgatga ccagtacgac gaaggtagtg
      301 aacgcgccga atgcgttgat cgcgatgttt cttcgccagt gggcgggctc ggcggtgccg
      361 agtgtcccgg ccgccttatg tgccttcagc cagtggatga ccatgcctgt ttgcgagagc
      421 gtgaaactcg tgaagactcc tacgacgtag agcgggatga tcctatgaac gagggccccg
      481 tacttgatca ggacgacgga ggcaccagca gccagaacca tgatgccgtt cgagaaagcc
      541 agcttgtcgc cacggttctg aaggaccctt ggcaggaatc tgtccctcgc aaggatcgaa
      601 gcgagtcgag ggaagtcggc gaatgctgta ttcgcggcca ggaacaggat cagggacgta
      661 aacgcctgga tgatgaagaa catcggagaa ttcgacccga agatctgccc cccgatctgc
      721 gacgtcacgg tcttgccatg ctcgatctgt atggggtcca cattcagcgc ccttgcaagg
      781 aatgtgatcc cgaggaagag gaagccgagc aggagaccga gcgtcaatag ggtctgcgag
      841 gcgttcttgg actctggccg cttgaatgcg ggcacaccgt cggagatggc ttccactcca
      901 gtcatggcgg tgcagcccgc agagaaagcc ttgagtatga ccaagacgct cagcccgact
      961 tctggcgtga tcgcgtgcgg cgggatcggc gcaactgtgt tcgttagaac cctgaacacg
     1021 ccgactccga tcagcacggc catcgacagg aggaatccgt aggtcgggat ggcgaagatc
     1081 gtgccggact ctttgaggcc cctcaggttg agcatggtga tgaaaaggac gatcagtagg
     1141 gaagccccaa ctctatggtt gtggatcgca gggaaggccg ctcccactgc agccaacccg
     1201 gcagcaacac tcactgatac ggtgaggacg tagtcgatga ggatcgccgc ggctgcgatg
     1261 agacctggga aggtgcccag gccgtccttg gtcacgatgt aggccccacc gccactcgga
     1321 tacgcccgca ccgtctggcg gtatgacgcg atcacaacgc ccagaaccag agctactgcc
     1381 agggagattc cgatcgagta ctgcaatgcg aggcccccgg cgagaacgag aacgagaagg
     1441 atctcgtcgg tcgcgtaggc gctagatgac agggcatcag aagagaagac tgcgagggct
     1501 accttcttgc tgagccggtg gtgcagttcc tcatgtgtcg agaccgggcg tccgaagaag
     1561 agtcgtttga agatcctgtt cacgagcagt cagcatacgg cccctaagct tcagcggcgt
     1621 cgatcttggt aggccttgcg accgaaggcc gactatagtt tcaactcatg catgcggtga
     1681 tcgtcggttg cgggcgggtg ggggccgagt tgggccaggc cctcgaggct cgcggagtat
     1741 cggtgtcgat catcgacagc gaccccaagt ctttcgagca gaggctccag cctggcttcg
     1801 agggaaagcg ccttgccgga atcggattcg acaaggacat cctcgaagag gcgggcatcg
     1861 agaaggccga gatattcgta tcggcgacca ggggagacaa caccaatatc gtctcggccc
     1921 gcatcgcaaa ggagcgctac ggagttcctc atgtggccgc tcttatctac gatccgcgcc
     1981 gtgctgagat ctacgagcgg ctgggtatcc gcacggttgc tactgtggcg tgggcaaccg
     2041 accagatcct ggcccgcatc atgcccgagc tggcatctgt ggagtggacg gtaggtggcg
     2101 gagaagtcgt ggttgtcggc gttacggtgc ccccttcgct catcggtaaa ccgatcgaga
     2161 tccttcgcga gccgggcaag atccatgtcg tggctcttac tcgtttcgga acgacccaga
     2221 tcccagccct taagaccatc atccaagaag gggatctcat ccatctctct gtcgtccgtg
     2281 acgcgttgtc ggaactcgat gagttgctga catcgaaagg ggatgtccat tgagggtcgc
     2341 gatcgcgggg gccgggaagg tgggccgatt catcgcgggc gatcttctga ggcggggcca
     2401 ccaggtgact ctcatcgacg ccgacggcga actcatcacg cgacaccgtg acgacctcaa
     2461 ctgtcgctgg gtgcagggcg atgcgtgtga gcccctcaac cttcaggggg ccgctctcga
     2521 tacctgcgag gtgatggtgg cagcgaccgg cgacgacaag gtcaatctgg ttgcctcgct
     2581 gctagcgaag caggagttcg gtatccctcg ggttctggcc agggtcaacc atcccaagaa
     2641 tgcgtggctc ttcaatgaga cctggggcgt tgatatcccg gtaagtgcta cccaccttct
     2701 gacttcgctc atcgaagaag cggtgaccgt cggcgacctg atcactttgc tgagactcga
     2761 gcaagggaag gtcttcttgg tggagtgcaa gctcggcgag aactcaccgg ttgctgggaa
     2821 ggtcttgcac gaggttacgc tcccgcgcga tagctcgatc gtcgccgtcg tgcgtcgtgg
     2881 gcatgtcatc cccccgcggg gcgatactcc gctgatgatc ggcgatgagg tgatggccct
     2941 gacgatccct gatgtgcaac gcgacctgga acagttactt acgggaacgc gcgggcctga
     3001 ggagcctggc gactaagggc ctagctttct acgatctcgt aggcggggtt gatgatctga
     3061 agcatctctc ctttgaaagt cgccatcgtg ccttcgagta tgagtcgctg gccgaggtcg
     3121 acgccgggga tctgctggcg cccgaaccag acacccatga tgcgatctgt cccgtcgaag
     3181 atctgcacct cgaggaccga ggtgtgttcc atgggcttca ctttgatgct ctcgacaacg
     3241 ccagcaacgc gtcgacgggt gcgagggcgg cactcggcga tctgctcgac cgcatcaagc
     3301 tccgcgcacc acaaccggat ctcttgcgcg tggtgctgtt ccggcgtctc cgccagacgg
     3361 tccccaaacc tcttgaacat ccccatgctt tcagggtacc gccgttgtct cgcaggtgcc
     3421 cgaaccgata gcctcgtctc tatggaccgc caagctgcgc tcgactacgt caggtcacgg
     3481 accgagaagg agacgacgct tcggcatctg atcacggtcg aggggatcat gcgaaggctg
     3541 gctgaacatc ttggcgagga tgctgatcgc tgggcccttg ctgggctctt tcacgacctc
     3601 gacatggagt tgacccatca cgatctcagc aaacacgcat atctcgcagc cgagtggctc
     3661 cgcgaggatg ggatcgacga ggacatcgtc aataccgttc tcgctcatgc gcatgagcag
     3721 taccgcacgg accgcatgtc gcaggggata gttcatgccg atgccgttgc ggggttcctc
     3781 gtggcttgcg cgctcgttcg gcctgagaag accgcgggga tgaaggtctc gtcggtgcgc
     3841 aagaagttga aggaaaagtc attcgcgccg ggggttgaac ggcccgagat attgaacgtt
     3901 gaggagaaga tgcagatacc gctcgatgag ttcctcgcca tcgcgataga gggcctgcag
     3961 tcggtcgccg aagaggctgg cctcagctag ttaagagagc cctgtacctc gtaagtaaat
     4021 agtcgagaaa cgaacggcgt acgaggatct aggctaggga tccgtccgag gaggtcaaat
     4081 atggcgcacc ccaacgagga actcgcacga agtgagatgg aggccgcagt ccaaggagac
     4141 tttgagggga tgcttgacta ctatacggac gacgcaatcc tccactaccc cggccggaac
     4201 gcgctatctg ggactcaccg cgggaaggac gggatccgga aatgggggcg taagttcggt
     4261 gagctccttg gggaagaagg atcgctcgcc cggacgctcc atgacatcgt ggtgagcgat
     4321 gatcacgcga tccagcttgt atcggttgag gctagacggt cggacggtcg gaaggctcgc
     4381 tggaatgttg cttgcgtaat gcacgtgcgc gacggcaaga tcagtgaggt ttggctgaat
     4441 atagatgacc cgtacgcggt cgatgagcta cttgcgtagc gattcttggc ctgaggcgta
     4501 gcgccgccca actgggccgt gcaggggcga gaaatctgga agcatatgaa aagtcatgcg
     4561 gacactgccc ctcacctgtt gagtgacctg accggtcgat tcagtgcacc cctgggacga
     4621 tgagatgatc tgttgatgag cgagggatct ggaatgcgcc acggtgagac ggctagcgac
     4681 tccgtgagag gaggatccct tcggacctcc gtccggcagc gtgccgatga gatcaagccg
     4741 tacagatgca agcacttgat cgcagtcatc gaagacccca gagacgtcaa gaacatcggc
     4801 acggtcctaa ggaacgtgaa cgctcttggc gtagagaagg cttacgttgt ggacccaagt
     4861 ggcagtctgc ccgacgattg gcaagagatg cgggaccaga ggtcactctc gaaaacgtct
     4921 gtgtccgcaa tcaaatggag tttcgttaag agattcgata gcacacaagc ctgcatcgac
     4981 caccttgaaa ggaatcgatt cgtttccatc gtcacatctc cacatgtgaa gggggagaac
     5041 aacgtcgctc taggtgaggc tgactacacc atctttccca agttggcggt ttggttcggc
     5101 aatgaagcca gggggatcag cgacctagct gtgcagcaca gcgagatgtg cgtgagcatt
     5161 cccatgttcg ggatgatcga gagcctgaat ctgggtacca cttcgggaat agtcttgcac
     5221 gaggtcacga aacagaggcg cgagtatcag cgtaagtatc gatggaggaa gaagcgcggc
     5281 agaaggatcg acgcctagga ttttcggcca ccccataaca aatccgtccg cacgcgctcg
     5341 ctccatcagt accttgcctt actcgaagag gccggtctcg accctcaggc ctggtatggc
     5401 ggattggatg gcactcctct cgaactggcg agccgacgac tcgtgttggt gagccgaaga
     5461 ggcgaccgcg atgaaccgag ctaacgcgcc ttcgctcgga tccgccgata gaaccagata
     5521 gcgatcaaca gaaggagccc tccggcgatg acgtatgtga tcgcgtcggc gtagggaagc
     5581 aggtccttcc atctctcgcc aagggcgaac cccacatatg tcagcgcgaa tgaccacggc
     5641 agacaaccaa caaaagtgag gagcgagaat cggaccagcg gcatgcgtgc gacacccgcg
     5701 ggcaccgaga tgaacgtccg cacgaccggc acaagtcgcg accagaacac tgcccggccc
     5761 ccatatcgct cgaaccatgc atccgctcgt tcgatgtcat gcggcttgac catcacgaag
     5821 cggccgtatc gaatcagcag agcccggccc ccgtacctcc ccatgccgta tgcgatccac
     5881 gatccgacga ggttgcccag ggttcccagc atcgcaacta gcacgaagtt gaagcgcccc
     5941 gcctgggcca gcaggcccgc caccggcatg gtgatctcac ttgggacagg gatcgcggcc
     6001 gattcgagga gcatcagaaa gaagatcgcg gcgtagccgg agggcccctt gatagaggct
     6061 tctatccata tcttcagcgc atcgagcagg tggcccatga ggcggcgatt ctagccgccc
     6121 cgccggacgt ggttcttgac aggacgtgaa aagggattca ctctccccat tcgttccccg
     6181 tttccagtca ggatctgact tgcccaacaa cttagtgatc gtcgagtcgc ctgcgaaggc
     6241 gaagacgatc gagaaatatc tcggctccga cttcaaggtg atcgcctcgg tcggacatgt
     6301 tcgcgacctg ccgaccaaga cgctggcggt ccacgtcgac gacgacttcc gactcgatta
     6361 cacggttaac gaggatcgaa ccaagatcat ctcctcgatc accaaggccg ccaagagcgc
     6421 agacgcggtc tatctcgcaa ctgactacga ccgcgagggc gaggcgatcg catggcacat
     6481 cacggaagct tgctccatcc cgctagagaa acagaagcgc atcaccttca ccgagatcac
     6541 caagaaggcc gtcaccgagg ccgtcgacca cccgcgctcg gtcgacccga tgctcgtaga
     6601 tgctcagcag gcccgccgct gcatcgaccg actcgtgggc tatccggtct cccaacttct
     6661 atgggagaag atccgctacg gcctctcggc cgggagggtg cagagcgtgg ctctgcggat
     6721 ggtggtcgag cgcgagcgcg agatcagcgc cttcatcccc ctcgagtact ggaccctcga
     6781 ggctctcttc gcgacggaca agaaggagac gttcgcggcg aagctagttc agatcggtga
     6841 acgcaagatc gcgacgcaga tctcgcgaga tgcccccgag gatgcggaag gaaggatccc
     6901 cgacgccgca caggcagagc agatcctcgc agccctagac ggcgcgacat atcaggtcga
     6961 ggacgtgcga acgcaggaga aacggcgatc gccccagccc ccattcatca cctcgacgta
     7021 ccagcaggag gcctctcgca agctggggta tggcgcacgc agggcgatgg gtatcgcaca
     7081 aggcctctac gaacgtggct tcatcactta tatgcgaacc gactcgacga ccatgagtca
     7141 ggaagcgatt ggcgctgctg agagcctgat ccgcaaggcc ttcggccccc agtactgggc
     7201 cggtcgatat tccaaccacg atcgcaagac gaagggggcc caagaagccc acgagtgcat
     7261 ccgccccacc gagatgagca tgccgagggc gcaggttgaa gccgcgatca gggccgaggc
     7321 ccctcgcgac tcagaagcga tgatcaaggt ctacgacctg gtctggaagc gagccgtctc
     7381 gtcgttgatg acgcaggctg tattcgatgc ggtctccgcc gacatagtca ccgcagggcc
     7441 gccacagaag catgtctttc gcgctaccgg ttcaacgatc aagttcgacg ggttcatgcg
     7501 tatctatatg gaggggcagg acgacgatga ggacgaggag ggcggcaagc tccccgccct
     7561 gaccgccact cagcctcttg acctcgaaga gcttcgcccc gatcagcact tcacggcgcc
     7621 cctgccgcgc tacaccgagg cgagtctgat caaggaattg gaatcgcgcg gcatcggtcg
     7681 cccctcaacc tacgcgtcga tcatggcaac cctggtcgaa gagaagcgcg gctacacgcg
     7741 cttggaccgt aaacgtttct tcgcaaccga cacgggcgag gttgtgagcg actacctcac
     7801 gcgattcttc ggtgaccact tcatggacta tcagttcact tctgacctcg aagaacatct
     7861 cgacgagatg gccgagggaa ggctcagcta ccgccctgtt gtcgagtcct tctatacacc
     7921 gctgaaagat cgtatggcca aggccgcaga gatcaccaag gaagaggtga cgacgcaggc
     7981 catcgacgag cgttgcccgg agtgcggctc gccgctcgtt atcaaactgg gaaggacggg
     8041 caagttcgtg gcttgcacca actacccaac gtgcaagttc acgcgcccac ttccggggca
     8101 ggagcaggcc cctcccgagc ttctcgacga ggactgcccg gactgcggca acaagctaca
     8161 gaaacgaacc ggaaggttcg gaccattcgt gggctgctcg acatacccgg agtgcaagta
     8221 catcaagcgc aagccgatca ccctcactgg ggtgccctgt cccaaatgcg cggaggcacc
     8281 atgcccatca tgcaagaagg gcaccgccgg cgaactcgtg gagcgcgatt ggaagagagg
     8341 caagttctac gggtgcaacc actatccccg atgcagatac ggtcagactc aggaccccag
     8401 aaccggcgac gtttccgccc ccgggtcttc cggagaggga aaggtaacta gcgcgccgtg
     8461 acggccaccg ggctcttcct cgtcttcgaa gggggcgagg gcagcggcaa gtcgacgcag
     8521 atccagagac taagaacctt cctcgaagag tccggacaca aggtcgtcgt gacccgcgag
     8581 cctgggggct catctgtcgg tgaggagctg cgacgactcg ttctgcagca tcgcgaccaa
     8641 cccatatcgc caaagaccga ggccctcatc tacgcggcgg accgcgctca gcatgtcgaa
     8701 gaggtcatca ggcccgcgct cgaggcgggg gctgccgtca tatccgaccg ttacgttgat
     8761 tcctcgatcg cgtatcaggg tctggcccgc ggactcggac tggagcagat cgagagcctc
     8821 aatcactggg gcaccggtgg actcctcccc gatctcgtct tcctcctcga cctcgacccc
     8881 gcagtgggcc tgcgcaggtc gggcgagacc gaccgcatcg aatctgaggg tgtggagttc
     8941 cacgttggcg tgagggacgc ctaccttcaa cttgctgacc gctatgcaga tagattccgt
     9001 gtcttggacg cgggactgcc cccaatggaa ctagagacac agatcaaggc gatagtgcag
     9061 tcgctgctcg aacgagcgga ggcgcgatga acaacatctg ggatcgatgc cctgtcgagg
     9121 gccgcacgcg tgccctgctc gagtcgtaca gccagcgatc cgggggttcg tatctgttca
     9181 tcggccccca gggggccggg atgtcagagg ccgccgagat cttcgccgcg gccctcatgt
     9241 gtcctgacga tgatggggat tgcgatatct gccgcaggac gatcgcgggg gtccaccccg
     9301 acgctcgctt cttcgaaccc gaaggcttca cctacccagt cgagacgatc cgtgagatca
     9361 caacatcggc atctcactct ccccttgaag gcagctcgcg tgtttttgtc atcgcagagg
     9421 ccgaccgcat cgccgagcgc tcgcagaacg ctctgctcaa gtctctcgaa gagccgaacg
     9481 cgtcggtcac ttgggtgttg ctcggtagat ccctggacat gttcctgccg acgatcctct
     9541 cgcgttgccg ggtcgtggag tttccggcga tgtcggagga tgcgatacgc gatgcgctgg
     9601 tagctgctgg cgaggttgcc cccgacccga tcgtgcgcgc cgcgcgcggg gatcttcaaa
     9661 gggcgatcgc actcgcgggc gagggtgccg cttcgcaact aaccgagctt gccaaacggg
     9721 tggtgctcga acccgagccc tcgccctcat gggtcctctc gatctccgat gcagtggcgg
     9781 ccgtcgccaa ggctcagcgg gaagttctgg agcagcagca ggcaactcaa ctcgaggagt
     9841 ctgacctggg caagggcgca ggcggggtcc gcaagcgcat cgccgaccgt cacaagcgcg
     9901 aggtgcgaag ggcagagagc gaggtcttca tgatgtttct ctccatcctc ggtgacacct
     9961 tcagggatct ggctgccaga ggagtggggg cccccgacga cgccctgatc ctgcaccgct
    10021 ctgagcttga tg
//
LOCUS       DATDOZ010000248         3926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2322342, whole genome shotgun sequence.
ACCESSION   DATDOZ010000248 DATDOZ010000000
VERSION     DATDOZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322342"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="dnaN"
                     /locus_tag="VI541_05035"
     CDS             <1..636
                     /gene="dnaN"
                     /locus_tag="VI541_05035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLF70301.1"
                     /translation="VLWSIEGETLRLVATDSYRLAIREVTLKEGGGERKAIIPSRALA
                     EFSRHLAGSNDTTAEIWLGETQAAFTAGKVRLVTRLIEGEFPNWRHLVPEGYESTLVV
                     EREGFQRAVERVGLVARANTPVRFHLAQEVQLTATESGVADASEIVEEAVYNGKDMVV
                     AFNPRFFIDGLEGLESEKAELQLIDPAKPAIIRAAGAEEYTYLLMPVRLPN"
     gene            655..1761
                     /locus_tag="VI541_05040"
     CDS             655..1761
                     /locus_tag="VI541_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HLF70302.1"
                     /translation="MEILHLTLKDFRNYETADVTFDGGLNIVVGPNGHGKTNLLEAVH
                     VMATMSSHRSATVGPMVRFDADTAIIRAQGKARARSVSVDAEIRRTGGVRVLVNKVPI
                     ERAEAASVISAVLFSPEDLNLTKGGPEERRRFLDQTCVKLRPLSGAERQEFERVLKQR
                     NGVLKAATSNPRAVKHLEVWTEQLIKTGSVVARNRLELLSRITGPIQRRHGEVAATGQ
                     PVTLGYEASWVQGAPPMSLDGISVSLRTAVEASLPRDIERGLSLVGPHRDDIAIRLDD
                     VDARTYASQGEQRSLALAMRLAERDILTEENGEEPILLLDDVFSELDEFRRSHLAELV
                     EVAGQTIATTTSDEGLGLAGRRLAVDSGKVVPLE"
     gene            1754..2308
                     /locus_tag="VI541_05045"
     CDS             1754..2308
                     /locus_tag="VI541_05045"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HLF70303.1"
                     /translation="MNEHVDAIAPLLNRVASKMGIERPAETARLFATWEAIVGPQIAL
                     RCQPTSLKEGVLRVRAQSPAWAAELRYLGPEVASRINAALGQKVVDQVKAWVPNPGGP
                     TSRFGRRRKGGRANSAGAEGEVEAGSWGSPDAAVTEPPEPLGVPSETDIAMAEEMTSG
                     IADERLAEITKRALLAGKMRRRQG"
     gene            2393..>3926
                     /locus_tag="VI541_05050"
     CDS             2393..>3926
                     /locus_tag="VI541_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF70304.1"
                     /translation="MSDEDRQNASGNSGAELPSESGAPAPKTEGTVPATKSPEKVAKA
                     STGKSRRAAGSYTGEDITVLKGLSAVRLRPGMYIGSTGPRGLHHLVYEVVDNAVDEAL
                     AGFCDRIDVTIHKDNSVTVVDNGRGIPVDPPKGEKRSAIEVVLTELHAGGKFGGKGYA
                     VSGGLHGVGVSVVNALSSRLAVEVTRDGGIWGAEFVRGKTVKPVSKTRSSNGTHTGSK
                     VTFQPDPEIFTETLEFDYDTIARRLRELSYLTRGVEITFKDERTDRKDVYKASGGIAD
                     FVKSLSSGRETLNKVIHFEKESDGRQAEVALMWNAGFTESIHSFANTINTHEGGVHEE
                     GLKKALTNVVNRYARGKGLLKEKEPAFEGNDIREGLVAIVSVKLREPQFEGQTKTKLG
                     NTEIRSVVETMVNEKLAEWFEENPTEARRIVAKAQIAARARISARKARDIARKSAFEG
                     GGLPGKLADCSSRDPAVSELFIVEGDSAGGSAKAGREREYQAILPIRGKILNVEKARL
                     HKI"
BASE COUNT          822 a         1225 c         1267 g          612 t
ORIGIN      
        1 gttctctggt cgatcgaggg cgaaaccctc cgactcgtcg ccaccgacag ctaccgccta
       61 gcgatccggg aggtcacgct gaaagaggga ggcggagaac gcaaggcgat catcccgagc
      121 cgggctctcg cagagttcag cagacacctc gccggcagca acgacacaac cgccgagata
      181 tggctcggag agacacaagc agccttcaca gcgggaaagg tgaggctggt aactcgcctc
      241 atcgaagggg agttcccgaa ctggcgccac ctcgtcccag aggggtacga gagcaccctg
      301 gtggtcgaga gagaaggttt ccagcgtgcc gtcgagcggg tcggactcgt tgcgcgggct
      361 aacacccccg tgagattcca cctagcacaa gaggtgcagc taaccgccac cgaaagtggg
      421 gtcgccgatg cctcggagat cgtcgaggag gcggtctaca acggcaaaga catggtcgtc
      481 gccttcaacc cccggttctt catcgatggg ctagagggcc tcgagtcgga gaaggccgaa
      541 ctacaactga tcgacccggc gaagccggcc atcatccgcg ctgcgggagc cgaggagtac
      601 acctacctac tcatgccggt gcggctcccg aattaggaag gtcggtggct cggtgtggag
      661 atcctccatc taaccctcaa ggatttccgc aactacgaaa cagctgacgt aaccttcgac
      721 ggcggcctca acatcgtcgt ggggccgaac ggtcacggca agacgaacct actcgaagcc
      781 gttcatgtca tggcaactat gtcctcgcac cggagcgcca ccgtggggcc gatggtccgc
      841 ttcgacgccg acaccgcgat catccgcgcg caaggtaagg ccagagcccg tagcgtaagt
      901 gtggatgccg agataagacg caccggaggg gtccgggtgt tggtcaacaa ggtccctatc
      961 gagcgcgcgg aggccgcgtc cgtcatatca gccgtcctct tctcacctga ggacctgaac
     1021 ctcacaaagg gcgggcccga ggagcgacga cggttcctcg accagacctg cgtgaagctg
     1081 aggcccctct cgggggccga gagacaggag ttcgaacgcg tcctcaaaca gagaaatggg
     1141 gtccttaaag cggcaacgag caacccgcgc gccgtgaaac acctcgaggt ctggaccgaa
     1201 cagctgatca agacggggtc ggtggtagca cgcaatcggc tggagcttct gagccggatc
     1261 accggcccca tccaacggcg gcatggcgag gttgctgcga ccggtcagcc agtgactctg
     1321 ggatacgaag cgagttgggt gcagggggcc ccaccgatgt ctctcgatgg catctcagtc
     1381 agtttacgaa ccgccgtcga ggcgtccttg ccgcgcgaca tcgagagggg cctctcgttg
     1441 gtcggccccc accgcgacga catcgccatc cgtctcgacg acgtcgatgc ccgcacttac
     1501 gcttctcaag gcgagcagcg ttctctggct ctggccatgc ggttggcaga gcgcgacatc
     1561 ctcaccgagg agaacggcga ggaaccgatc ctcctgctcg acgacgtctt ctccgagctc
     1621 gatgagttta gaaggtcaca tctagcggaa ctggtcgagg tcgccgggca gaccatagcg
     1681 accacgacgt ccgacgaagg tctcggcttg gcggggcgca ggctggctgt ggactcgggc
     1741 aaggtggtgc cgcttgaatg aacatgtcga tgcgatagct ccgctactca atcgggtcgc
     1801 ctcgaagatg gggatcgaaa gaccggccga gacggcgcgc ctgtttgcaa cctgggaggc
     1861 gatcgtcggc ccccagatcg cgctccgctg ccagccgacg tcgctcaaag aaggggtact
     1921 ccgagtgaga gcgcagtcac cagcatgggc ggctgagctt cgttatctcg gacccgaggt
     1981 ggcgtcgcgc atcaacgccg ccctggggca gaaggtggtc gatcaggtca aggcgtgggt
     2041 gccgaacccc gggggcccaa cctcaagatt cggtcgccga aggaagggcg gtcgcgcgaa
     2101 cagtgccggt gcagaaggcg aggtcgaggc gggctcttgg gggtccccgg atgccgccgt
     2161 caccgagccc ccggagcccc tcggtgtccc gtccgagacc gacatcgcga tggccgaaga
     2221 gatgacctcg ggcatcgccg atgagaggct tgctgaaatc acgaaacgtg cccttctagc
     2281 agggaaaatg aggcggaggc aggggtagcg acatggtata cttcggcagg ttccaaaccc
     2341 ccttttctgg ggactgtgga tcacctaaac cggcccccag gaaggcacct agatgagcga
     2401 cgaagatagg caaaacgcgt ccggcaacag cggcgcggaa ctcccctccg agtcaggtgc
     2461 cccggccccc aaaaccgaag gaaccgtccc cgcaaccaag agccctgaga aggtggcgaa
     2521 ggcctctacc gggaagtccc gcagggccgc cgggagctac acgggcgagg acatcacggt
     2581 cctcaagggg ctcagcgccg tccgtcttcg tccaggcatg tacatcgggt cgaccgggcc
     2641 tcgcggtctt caccacctgg tctacgaagt agtagacaac gcggtcgacg aggccctagc
     2701 cggtttttgc gatcgcatcg acgtgaccat ccacaaggac aactcggtga ccgtcgtcga
     2761 caacgggcga ggcatccccg tcgacccccc caagggcgag aaacgctccg cgatcgaggt
     2821 cgtcttgacc gagttgcacg ccggtgggaa gttcgggggc aagggttatg cggtttccgg
     2881 tggcctgcac ggcgtcggag tctcggtggt caacgctctg tcgagccgcc tcgcggtcga
     2941 ggtgacccgc gatggcggta tctggggggc cgagttcgtt cgcggtaaga ctgtgaagcc
     3001 cgtcagcaag actcgctcgt cgaacgggac ccacaccggg tccaaggtga ccttccagcc
     3061 cgaccccgag atcttcaccg agaccctgga gttcgactac gacaccatcg cccgacgtct
     3121 gcgggagctc tcctacctca ctcgcggtgt cgagatcaca ttcaaggacg aacgcaccga
     3181 ccgcaaagat gtctataagg ccagcggggg catcgccgac ttcgtgaagt cgctctcatc
     3241 ggggcgcgag acgctgaaca aggtcatcca cttcgagaag gagagtgacg gccgccaggc
     3301 cgaggtcgcg ctcatgtgga acgcaggctt caccgagtcg atccactcat tcgcgaacac
     3361 gatcaacacc cacgagggcg gcgtgcatga ggagggcctg aagaaggctc tgaccaacgt
     3421 ggtcaaccgc tatgcccgag gcaagggtct tctgaaggag aaagagccag ccttcgaagg
     3481 caacgacatc cgcgaaggtt tggtcgcgat cgtctccgtg aagttgcggg agccgcagtt
     3541 cgaaggccag accaagacga agctcggcaa taccgagatc cgctcggtcg tcgagaccat
     3601 ggtcaacgag aaactcgcag agtggttcga agagaaccca acagaggcgc gccgcatcgt
     3661 ggcgaaggct cagatagcgg cgagggcacg gatctctgcc cgcaaggccc gcgacatcgc
     3721 ccgcaagtcg gcgttcgagg gcggtggcct ccccggcaaa ctcgccgact gttcatctcg
     3781 cgacccagcg gtctccgaac tcttcatcgt cgagggcgac tctgcaggcg gcagtgccaa
     3841 ggccggacgc gaacgcgagt accaggcgat cctccccatc cgcggcaaga tcctgaatgt
     3901 cgagaaggcc cgccttcaca agatcc
//
LOCUS       DATDOZ010000249         2972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_726371, whole genome shotgun sequence.
ACCESSION   DATDOZ010000249 DATDOZ010000000
VERSION     DATDOZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726371"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VI541_05055"
     CDS             <1..557
                     /locus_tag="VI541_05055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF70305.1"
                     /translation="MVQRWAAQGKERSPASELVLYEDRLPDDVLEKYTPVITAVLNTV
                     PFEELDHGDIVITPEMVKEWNRQMDAVGDSHHTYVTIEPDGDYSAMTDVFYSPHQPTY
                     VWQGFTGVKPEYRGRGMGRWIKAAMLLYLRGRYEGLRWVVTDNAHSNSWMLAINEELG
                     FSRYKAGSTYQISKEVLDKYLANV"
     gene            569..1621
                     /locus_tag="VI541_05060"
     CDS             569..1621
                     /locus_tag="VI541_05060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF70306.1"
                     /translation="MSTQTWVIEQFDPKTASAQEWERYHAYRKTRHVERERDEPLFPD
                     DQVETMMKMDDPYSKPFHFGISDGDVIVSSFGGGIATPEAPGYDSNKQYFWFGGSVLK
                     GYRRRGIGKLWLPKLLELMQETETTVATTGTDEPDGNEFLRWAGFEGKQEGAENRLKW
                     DEIDWELVDSWIAEGQKRSPDSKLEFYENRLPDAFLEEYATAMTPLLNTMPFDDLDHG
                     EIVFTADVLREHYKRGDQIGAEHHVALIREPDGSISGMTDVVWLPPQAAFVQQQFTGV
                     HPDARGRGLGKWLKAYMLKFLKGRYDGIEWVATGNANSNDPMLGINKRLGFKEHRPNV
                     AYQISRDTLADFVNKL"
     gene            1630..2658
                     /locus_tag="VI541_05065"
     CDS             1630..2658
                     /locus_tag="VI541_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70307.1"
                     /translation="MIEFRPIEAERSLWAAYHAFRADRHAEVDPSDPLPPDEHVEKAL
                     RFADPHQQILRYLFLDGDGVAGSLTLWRVLPASPEYEEHRRFMRVDAQIRSGNRGQGI
                     GLDFARKVLATAKEWEKEVITASTHEETGQRFLARLGAKARYREAESRLYMSEVDWDQ
                     VQRWLDEGLARNPGISLELFPTDVPDELLDAYLPVLTEINNDMPDEEMETGEDILDRQ
                     TWEILRDWLANREARHPTVLTLEPDGEVSALTDVMFEKAVPHAIHQWATGVRKHHRGR
                     GLGKLVKAKMLLYLREQCPEALYVKTENARSNDAMLHINIAMGFRFHREHVAYQMKTA
                     ELDESLAL"
BASE COUNT          650 a          822 c          942 g          558 t
ORIGIN      
        1 agatggttca gcgttgggca gcgcagggca aggagcgcag cccggcttcg gaactggttc
       61 tatacgagga tcggcttccc gacgacgttc tcgagaagta cacgccggtg ataacggcgg
      121 tcctcaacac ggtcccgttc gaggagttgg atcatggcga catcgtcatc actcccgaga
      181 tggtcaagga gtggaaccgc cagatggatg ccgttggtga ttcgcaccac acctacgtga
      241 ccatcgaacc ggatggtgac tactcggcga tgactgatgt cttctactcg ccgcaccagc
      301 cgacatatgt ttggcagggc ttcacgggcg tgaaacccga gtatcggggc cgcgggatgg
      361 gtcgctggat caaagcagcg atgctgctct acctgcgcgg tcgctacgag ggccttcgct
      421 gggtggtgac cgacaacgct cactcgaaca gctggatgct tgcgatcaac gaggagttgg
      481 ggttcagccg atacaaagca ggtagcacct atcagatcag caaggaagtg ctggacaagt
      541 atctggccaa cgtatgaatg ggggagagat gagcacgcag acgtgggtta tcgagcagtt
      601 cgacccaaag accgctagtg cgcaggagtg ggagcgttac cacgcctatc gcaagactcg
      661 gcacgttgag cgcgagcgcg acgagccgct gttccccgac gaccaggtcg agaccatgat
      721 gaagatggac gacccctaca gcaagccgtt ccacttcggg atcagtgatg gcgacgtcat
      781 cgtgagtagt ttcggcggcg ggatcgcgac acccgaggcc cccggctatg actcgaacaa
      841 gcagtacttc tggttcggcg gaagcgttct caagggatat cgccggcggg gcatcggcaa
      901 gctgtggctc cccaaactgc tcgaactgat gcaggagacc gagactacgg ttgcaacgac
      961 cggcaccgat gagcctgacg gcaatgagtt cctccgctgg gcagggttcg agggcaagca
     1021 agagggtgcc gagaaccgat tgaagtggga tgagatcgac tgggagctcg tcgactcatg
     1081 gatcgccgag ggtcagaagc gttcacccga ctcgaagctc gagttctacg agaacagact
     1141 gcccgacgct ttcctcgagg agtacgcgac cgcgatgact ccactgctga acacgatgcc
     1201 cttcgacgac ctggatcatg gagagatcgt cttcaccgcc gatgttcttc gcgagcacta
     1261 caagcgcggc gaccagatcg gtgccgaaca tcacgtcgcg ttgatccgcg aacccgatgg
     1321 ctcgatatca gggatgaccg acgtggtctg gctcccgccg caggcagcat tcgtacagca
     1381 gcagttcact ggggtgcacc cggatgctcg cgggcggggc ctgggcaagt ggctcaaggc
     1441 atacatgctg aagttcctga aggggcgtta cgacggcata gagtgggtcg ccaccggcaa
     1501 cgccaactca aacgacccta tgctcggcat caacaagcga ttgggtttca aggaacatcg
     1561 accaaacgtt gcctaccaga tctcgcgaga cacgctggcg gacttcgtaa acaagctctg
     1621 agggtggggt tgatcgagtt tcgccccatc gaggcagagc ggtctctctg ggcggcgtac
     1681 cacgccttca gggcagaccg gcacgccgag gtggacccct ccgatcccct gcccccggat
     1741 gagcatgtcg agaaggcgct gcgtttcgcg gatccgcatc agcagatctt gcgttacctc
     1801 ttcttggacg gcgacggggt cgctgggagc ctcaccctct ggcgggtcct tcccgcatcg
     1861 ccagagtacg aggagcatcg aaggttcatg agggtggatg cgcagatccg ttcgggcaac
     1921 cgggggcagg ggatcggcct cgactttgct cggaaggtgc ttgccacggc gaaagagtgg
     1981 gagaaagaag tcatcaccgc gtctacgcat gaggagaccg gccaacggtt cctcgcgcga
     2041 ctgggggcca aggctcgcta tcgcgaggct gagagccgcc tttacatgag tgaggtcgat
     2101 tgggatcagg tgcaacgttg gctggacgag gggttggcca ggaaccccgg tatctcactc
     2161 gagttgttcc cgacggacgt gccggatgaa cttctcgatg catatctgcc tgtcctcacc
     2221 gagatcaaca acgacatgcc tgacgaggag atggagaccg gcgaggacat cttagatcga
     2281 cagacatggg agatcctccg agattggctt gcgaatcgtg aagcgagaca tcccacggtt
     2341 ttgacgctag agcccgacgg cgaggtttcg gctttgaccg atgtgatgtt cgagaaggcc
     2401 gtgccgcatg ccatccatca gtgggccacc ggggttcgca agcatcatcg aggccggggg
     2461 ttggggaagc tagtcaaggc caagatgctt ctgtatctgc gagagcagtg tcccgaagcg
     2521 ctctatgtta agaccgagaa cgcccgatcg aacgatgcga tgcttcacat caacatcgcg
     2581 atggggtttc gatttcatcg tgagcatgtt gcttatcaga tgaagacggc cgagctcgat
     2641 gagtccttag ccttgtgacc catttcacaa gttagtggct gagaaacatc aatctagtct
     2701 ctgagcaggg agaatgtcac cgcggtgacc ctaacgttag gctagcctaa cctcctgttc
     2761 actgctaaac tcaggctaca gctgcggcag gctccggcgg cgaatcgact ttcttgaagg
     2821 ggtgtcccga aatggccata gaagacctag atccggttct gaaagagaac gaagaggtca
     2881 gggcgctcaa ggacggttac aagtacggct ggcacgacgc cacctcgtat gacgaggccc
     2941 ccacgagggg tctatcgcat caggtgctcg ac
//
LOCUS       DATDOZ010000250         3044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3075965, whole genome shotgun sequence.
ACCESSION   DATDOZ010000250 DATDOZ010000000
VERSION     DATDOZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075965"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VI541_05070"
     CDS             complement(<1..944)
                     /locus_tag="VI541_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF70308.1"
                     /translation="MVGGGVVGSATALELARRGHEVHLWERFAPGHHRGSSHGPVRIF
                     RLTYPDPDYVALGLQALEDWRRLERDARAPLIASTGGIDFGEGVEVCVKALTQMGVPF
                     DELDGSEVKRRWGVTAPSGPIIHVPDNGVIAAEVTVRKQIELAQRAGVDVSYLTPVTA
                     IEGDDPVKVTTQERDVDVECLVICAGAWAAPLAQMVGIGLPLMVTCEQVAYLGPTPAI
                     VPPVLIDWTEPARYCVPAMHGAVGLRVGIHHGGKVVDPQDGPFEPDEALTRAAVAWGA
                     EILGVSLEPIAVETCLYTNAPNEDFILERHGGVVVVSA"
     gene            complement(956..1453)
                     /locus_tag="VI541_05075"
     CDS             complement(956..1453)
                     /locus_tag="VI541_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLF70309.1"
                     /translation="MEGFTVLQACRFTGCTTHQLRYWDQIGLVKPGVQATGGRPGVRR
                     LYAFRDLVALKVVKALLDGGMSLQKVRRAYEYLRKKAGLEQHLADVKLITDGQSIFRV
                     AKDDGEVLDALRQGQMAFFLAIDEITRNLDQGVAEFLRDREEFVQTLRQAEDHLMEER
                     ARASR"
     gene            complement(1602..1973)
                     /locus_tag="VI541_05080"
     CDS             complement(1602..1973)
                     /locus_tag="VI541_05080"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HLF70310.1"
                     /translation="MPLSDREQKILDEIERNLLEHDPKFARGVATKTTSTSNAQVRLG
                     VALFAVGFIALIAFFITAAVPVGVGGFVLMLAGATLALRGASVAVARRRSGTQRASLS
                     DIFVRMEGRLRDMRGGGDDRD"
     gene            complement(2001..>3044)
                     /gene="dinB"
                     /locus_tag="VI541_05085"
     CDS             complement(2001..>3044)
                     /gene="dinB"
                     /locus_tag="VI541_05085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLF70311.1"
                     /translation="PARAFGVRSGMPTSRARRLCPGGIFIQPDFDSYMSKSKQVREVF
                     NSFSSIVEPVSLDEAFIDVSRARRMWANPPTIAEALRDRVMRETGLVVSVGVAPNRFL
                     AKVASRRAKPDGIVVVREGDVEAFLHPLHVGELWGVGEQTVLILERLGLRTVGDVAAL
                     PRETLERALGSLGAHLADLAAGRDDRPIVPNVIRKSLGADETFEQDLVQELQMAQALL
                     KLSDRVCTRLRSQGISGRTVTLKVRFGNFATLTRSRSLPHEIDSTSGIFGVARDLLRQ
                     VLGDREPGTKRIRLLGITMSNLSEWPASEQLTFDRAPNWSQADRAIDRVRFRFGDDAV
                     GFGSLLEDPYNQF"
BASE COUNT          599 a          986 c          889 g          570 t
ORIGIN      
        1 caagcggaga cgaccactac cccgccatgc ctctccaaga tgaagtcttc attcggcgca
       61 ttcgtataga ggcaggtctc gacagctatc ggttcgagac tcacccccag gatctcggcc
      121 ccccacgcga ccgctgcccg cgtgagagcc tcatccggtt cgaacggacc atcctgcggg
      181 tcaaccacct tcccaccgtg atggatgcca accctcaggc cgactgctcc atgcatcgct
      241 gggacgcagt agcgcgctgg ttctgtccaa tcgatcagga cggggggcac gatcgctggg
      301 gtcgggccta ggtaggccac ctgttcacac gtgaccatca gcggaagacc gatccccacc
      361 atctgggcga ggggtgccgc ccacgccccc gcgcatatga cgagacactc gacgtccaca
      421 tcgcgttcct gcgttgtgac cttcaccgga tcgtcgcctt cgatagcggt cacgggtgtg
      481 aggtaactga catcgacccc ggcacgctgc gcgagctcta tctgtttgcg cacggtgact
      541 tcggcagcga tgactccgtt gtccggcaca tggatgatcg gcccggaggg cgccgtcact
      601 ccccatcttc gtttgacctc gctgccatcc aactcgtcga acggaacacc catctgggtg
      661 agggccttta cgcagacctc cacaccctcg ccgaagtcga tacccccggt ggaggcgatc
      721 agaggtgcgc gagcgtcgcg ctccaacctc ctccaatcct cgagagcctg gagccccaga
      781 gcaacatagt cggggtccgg ataggtaagc cgaaatatcc gtaccggccc atgcgatgac
      841 ccacggtgat gtcccggggc gaagcgctcc cagaggtgaa cctcgtgtcc gcgtctagcg
      901 agctcgaggg cagtggcgct gccgacgacc cctccgccga ccacaccaac cgtagctacc
      961 ggcttgccct ggccctctct tccatcaggt gatcttcagc ctgacgaagg gtctgaacga
     1021 actcctcgcg gtccctcagg aactctgcaa ctccctggtc gaggttccgc gtgatctcat
     1081 cgatcgcaag gaagaaggcc atctggccct ggcggagggc atcgaggacc tcgccgtcgt
     1141 ccttcgctac gcgaaagatg ctctggccat ctgtgatgag cttgacatcg gccagatgct
     1201 gttcgagacc tgccttcttg cgaaggtatt cataggccct gcgaaccttc tggagcgaca
     1261 tcccgccatc cagcagagcc ttgacgacct tgagagcaac caggtcgcgg aaggcataca
     1321 gacgcctgac gccgggccga cccccggtcg cctgaacccc aggtttgacc agaccgatct
     1381 gatcccaata ccgaagctga tgagtcgtac agcctgtgaa tcgacaagct tgaaggacgg
     1441 tgaacccctc catcgtgtcc tttccgaaac taaagattgt gtgccactga gtagaagaga
     1501 gagttggtag agcgaggcca tgttctaaca aagagagcat gcttcgcgca aggcgcgcgt
     1561 tagatctttt tcaagttaga aacgaatttg ttcgcggttt ctcagtcgcg gtcgtcgccg
     1621 ccacctcgca tatcgcgaag gcgaccttcc atgcgaacga agatatcgga gagtgaggcc
     1681 ctctgagtgc cgctgcgtcg tctcgcgaca gccacactgg cgccgcgcaa cgctaaggtt
     1741 gcccccgcaa gcatcaagac gaatccaccg acaccaacgg gtaccgcggc cgtgatgaag
     1801 aatgctatga gcgcgatgaa accgacggcg aacagagcga ccccgagccg gacctgagca
     1861 ttcgaggtcg aggttgtctt cgtcgccaca cctcgcgcga acttaggatc atgctcgagg
     1921 aggtttcgct ctatctcatc gagtatcttc tgttcacgat cggatagtgg catcacatgc
     1981 tcccggtcac accggccctt ctagaactga ttatacgggt cttcgagcag tgacccgaac
     2041 ccaactgcgt cgtcgccgaa tctgaacctc accctatcga tggcgcggtc ggcctgcgac
     2101 cagttggggg cccgatcgaa ggtcaactgt tcagaggcgg gccattcgga gaggttgctc
     2161 atggtgatcc ccagtagacg gatccgcttg gtccccggct ccctatctcc taaaacttgt
     2221 ctgagtaggt ctcgagccac tccgaagatt cccgacgtgc tgtcgatctc atgggggagg
     2281 ctgcgggacc gagtcagggt ggcgaagttt ccgaatcgga ccttgagggt gaccgttcgc
     2341 ccgctgatcc cctgggagcg caggcgggtg cagacacggt ctgagagttt caggagggcc
     2401 tgagccatct gaagctcctg gacgaggtcc tgctcgaagg tctcatctgc cccgagggac
     2461 ttcctgatca cgttgggaac gatcgggcgg tcgtcacgcc cagcggccag atccgccaga
     2521 tgtgccccca gggagcccag tgctcgctcg agggtctcgc gcggaagggc ggctacatct
     2581 ccaaccgtcc gcaggcccag ccgttcgagt atgagcaccg tctgctcccc cactccccat
     2641 agctcgccca catgcagcgg atgtaggaat gcctcgacgt ctccctccct caccacgacg
     2701 atgccatcgg gcttggcgcg cctcgatgcg actttcgcga ggaatcggtt gggggcaacg
     2761 ccgactgaca ccaccagccc cgtctcgcgc atcacccgat cgcgaagggc ttccgcgatg
     2821 gtgggcgggt ttgcccacat ccgcctggct cgactcacgt ctatgaaggc ctcgtcaaga
     2881 gagacgggct cgacgatcga cgagaaggag ttgaagactt cgcgaacctg tttcgactta
     2941 ctcatgtatg aatcgaagtc gggctggatg aatatcccac cgggacagag ccggcgggct
     3001 cgcgaggtcg gcatccccga gcggaccccg aacgcgcggg ccgg
//
LOCUS       DATDOZ010000251         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2058310, whole genome shotgun sequence.
ACCESSION   DATDOZ010000251 DATDOZ010000000
VERSION     DATDOZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058310"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..817
                     /gene="prcA"
                     /locus_tag="VI541_05090"
     CDS             137..817
                     /gene="prcA"
                     /locus_tag="VI541_05090"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HLF70312.1"
                     /translation="MALPYYVSPEQFMKDKSDYARKGIARGKSVIALDYADGIAFVAE
                     NPSATLHKISEIYDRMAFAGVGKFNEFETLRMHGIRQADVRGYSYSREDVNARGLANS
                     YSQILGQIFTEAMKPFEVEILVAQVGREGESNELYQIRYDGSVEDEENFVAMGGEAEQ
                     LTAYLQEHFAPGQSAAAAVKLGASALGAVQTEAVAVSNLEVGVLDRNLARRKFRRISP
                     AELEGMLA"
     gene            874..2226
                     /gene="pafA"
                     /locus_tag="VI541_05095"
     CDS             874..2226
                     /gene="pafA"
                     /locus_tag="VI541_05095"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HLF70313.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDNVASLVAHDKAGERILESLVTAAEMRLRDEGI
                     AGQVYLFKNNTDSAGNSYGCHENYLVSRVGEFGKLADSLIPFFVTRQIYSGAGKVLQT
                     ARGAIYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSEY
                     STWIKVATTAVLLRMLEEGSPLRDLTLENPIRAIREISHDMTCRRKVRLASGREATAI
                     EIQWEYLSRAAKFAERRDDADMKQAVTAWEECLVALEEDHTRLDTKVDWVMKQRLLER
                     YRTKHGIGLGHPKVALMDLTYHDVSRRRGLYYALERKGLAERHVSEEDVEVAMSDPPQ
                     TTRAKLRGEFIRAAKKRRRDYTVDWVHLKLNDQAQRTVLCKDPFKYSDDRVARLISSL
                     "
     gene            2246..>2767
                     /locus_tag="VI541_05100"
     CDS             2246..>2767
                     /locus_tag="VI541_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HLF70314.1"
                     /translation="MPKFETGVVSAIVGTRPGLIRAKAKVGRRVREITAFTDVIGDIG
                     VGDRVLINTTGVDLDLGTGGQDFVVWNLERDKGGSLSGGHILKLRYTPWQIDTMVAEA
                     PESQHHASLQSATSLDGMPVVACGLHSQIAPVAAMLKHLDPDLRIGYIMTDGAALPLR
                     HSDLVVTLKEKGLI"
BASE COUNT          604 a          799 c          843 g          521 t
ORIGIN      
        1 ggcagagggc ttcgtcgaga tcccagattc cgagatcgcg tcgctattcc agatgctcat
       61 ggaaagccgg aggtcacagg gggcagacct gcgcaaagag accgttccgg gctcgaccga
      121 acatcaggga gattagatgg cacttcctta ctacgtttcg ccagagcagt tcatgaagga
      181 caagtccgac tacgctcgca agggcatcgc ccgaggaaag tcggtcatcg ctctcgacta
      241 cgccgacggc atcgccttcg tagccgagaa tccatctgca acgctacaca agatctctga
      301 gatatacgac cgcatggctt tcgctggcgt tggtaagttc aacgagttcg agaccctgcg
      361 tatgcatggg atcaggcagg ccgatgttcg gggctactcg tactcccgtg aggatgtgaa
      421 cgctcgaggc ttggccaact cctactcgca gatcctaggc cagatattca ccgaggcgat
      481 gaagcccttc gaggtcgaga tcctcgtggc ccaggtcggg cgcgaaggcg agagcaacga
      541 gctctaccag atccgctacg acggttctgt tgaagacgaa gagaacttcg ttgcgatggg
      601 aggtgaggct gaacagctga ccgcctatct gcaggagcac ttcgccccag gacagagcgc
      661 cgcggcggcg gtgaagcttg gggcgtccgc gttgggggcc gtccaaaccg aggccgttgc
      721 ggtctccaat ctcgaggtag gtgtcctcga tcgcaacctc gcccggagga agttccgtcg
      781 catatcgccg gcagaactag aggggatgct cgcgtaacct ggcccttctg gtgaaagcca
      841 gtgacctgga cggcaccgtg tcatcatcca gggatggagc gtcgcatctt cggtctggag
      901 aacgagtacg gggtgacctg taccttccgt ggacaacgca ggctgtctcc cgacgaagtc
      961 gcgcgttacc tattcaggcg tgttgtctcg tggggacgat cgtcgaacgt ctttctcgag
     1021 aatggcgctc gcctctacct ggacgttggt tcgcaccccg agtacgcgac gccggagtgc
     1081 gacaacgtag ccagtctcgt ggcccacgac aaggcaggag agcgcatcct cgaatctctg
     1141 gtcactgctg ccgagatgcg tcttcgcgat gaagggatcg ccggccaggt ctaccttttc
     1201 aagaacaaca cggattcagc aggcaactca tacggatgtc acgagaacta cctggtcagc
     1261 cgggtcggag agttcgggaa gttagcggat tcgctcatcc cattcttcgt gacccggcag
     1321 atctactctg gggccgggaa ggtcttgcag acggcaaggg gagctatcta ctgcctctct
     1381 cagcgggccg agcacatctg ggagggtgtg tcatcggcga cgacccgctc gcggccgatc
     1441 atcaatacgc gcgatgaacc acatgccgac gccgagaagt atcgccgctt gcacgtgatc
     1501 gtcggcgact cgaacatgag cgagtactca acttggatca aagtcgcgac cactgcagtt
     1561 cttctgcgaa tgctcgaaga aggcagccca ctcagagacc tgaccctgga gaaccccatc
     1621 agagctatca gagagatctc gcacgacatg acctgtagaa gaaaggtgag actcgcgagc
     1681 ggccgtgagg cgaccgccat agagatccaa tgggagtatc tgagccgcgc tgccaagttc
     1741 gctgaacgaa gagacgatgc tgacatgaag caagcggtta cggcttggga agagtgcctg
     1801 gtcgcactcg aggaggatca cacaaggctc gataccaagg tcgactgggt gatgaagcag
     1861 cggttgctcg agcgatatcg aaccaaacat ggcatcggtc tcggacaccc caaggttgcc
     1921 ttgatggacc tcacttatca cgacgtgagc aggaggcgag gtctgtatta cgccctcgaa
     1981 cgcaagggcc tggccgagcg tcatgtgagt gaggaggacg tcgaagtagc catgtccgat
     2041 cctccccaga ccacgcgagc caagttgcgt ggggagttca taagagcggc taagaagcgc
     2101 cggagggact acacggtcga ttgggtccac ctcaaactca acgatcaggc ccagagaacg
     2161 gtgctgtgta aggacccctt caagtactcg gatgatcgtg tcgcccggct gatctcttcc
     2221 ctctgaccca gactagactt cggccatgcc aaagtttgaa accggcgtgg tcagcgctat
     2281 cgtcgggacg cggccaggcc tcatccgtgc caaggccaag gtgggccgtc gggttcgcga
     2341 gattaccgcc ttcactgatg tcatcggcga catcggtgtc ggagacaggg tcttgataaa
     2401 cacgacaggg gtcgatctgg acctggggac cggtggtcag gacttcgtcg tctggaatct
     2461 cgaacgcgac aaaggtggaa gcctctccgg gggccacatc ctcaaacttc gctatacgcc
     2521 ctggcagatc gacaccatgg tcgccgaggc ccccgagagc caacaccacg ccagtttgca
     2581 gtcggcaacc tcactcgatg gaatgccagt cgtagcgtgt ggccttcact cacagatcgc
     2641 tcctgtggcc gccatgctca agcacctcga tccagacctt cggatcgggt acatcatgac
     2701 cgacggcgcg gccctgcctc tcaggcacag cgatcttgtc gttacgctga aggagaaggg
     2761 cttgatc
//
LOCUS       DATDOZ010000252         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2325777, whole genome shotgun sequence.
ACCESSION   DATDOZ010000252 DATDOZ010000000
VERSION     DATDOZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325777"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VI541_05105"
     CDS             complement(<1..300)
                     /locus_tag="VI541_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70315.1"
                     /translation="MKIKTLAPRVVGLLCMATTVVVGSPARADIVRYLDPIFAVPTDP
                     ITSLATVTYGSAPKFGGGTSTLLLDVWEGVGDTATGRAAIVFAHGGGFSNGTRFGP"
     gene            453..833
                     /locus_tag="VI541_05110"
     CDS             453..833
                     /locus_tag="VI541_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70316.1"
                     /translation="MKMRTKILPLVLIATFVLGSTAVARADDETSRGAGPSPERCAAK
                     LAKLESSFEKQTAKLQAQFDRKKAKLAEQLAKHTDDPEAQARFERKQARLQAWFDKKS
                     GKLNARHDRKKAKLGSKCGAPATA"
     gene            893..1387
                     /locus_tag="VI541_05115"
     CDS             893..1387
                     /locus_tag="VI541_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70317.1"
                     /translation="MKRIVLVLALLLVGASCTRTPDKHPRPLPVQQGQSDISVAGSCV
                     ETYSLETLKDREIAFDGVVAEVVTTESPSSVQGEGPETRYDVRFDVNRWFKGGSGDAT
                     TLRSSIPLGDGDGAVTSVDGIAITKGSRYLVSGDGGFIWSCGFTSEYSEAEAASWANT
                     LGAG"
     gene            complement(1384..2235)
                     /locus_tag="VI541_05120"
     CDS             complement(1384..2235)
                     /locus_tag="VI541_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLF70318.1"
                     /translation="MSLDAARETSRAIWDEMSAGWELKSDFLWEAGRKIGEWMVEKVD
                     PKGGQTILELAAGPGMTGFVAAKLVGDDGKLISTDFAPAMVKVAEKTAARLGVTNAEF
                     RVMDAEAIDLPDASVDGVLCRWGLMLMMDPQTALKEIRRVLKPGGKLAFSVWGAARDN
                     IWAIIAGRVMVSLGKLTPPDPQAPGGMFSMAEPSVVEGMMGDAGFVDWKIEPVSAEWR
                     FADFDDYWSFSRAIAGAIALLLRDMDEAELIEVREAVRAEIEAFRDGDGYLLTGVAIN
                     AVATAPS"
     gene            complement(2259..>2594)
                     /locus_tag="VI541_05125"
     CDS             complement(2259..>2594)
                     /locus_tag="VI541_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HLF70319.1"
                     /translation="IPFMGGAVGRVGAKDTAFGDRSAPFMVSIDGNWWDPAEEKQVIS
                     WVRDCFSDMKRFSTGQAYLNFTGKEESDKDAIVRSAYGDNYARLQELKKKYDPQNFFR
                     LNQNIEPAS"
BASE COUNT          511 a          806 c          760 g          517 t
ORIGIN      
        1 gggcccgaag cgagttccgt tcgagaatcc cccgccatgc gcgaacacga tggcagccct
       61 gccggtcgcc gtgtcgccaa ccccctccca aacgtctaga agtagagtcg aggtcccgcc
      121 cccgaacttg ggcgcggatc cataggtgac agtggccagg ctggttatcg gatcagttgg
      181 gactgcgaag atggggtcca ggtatcggac gatgtccgcc cgagccgggg accccaccac
      241 gaccgtcgtt gccatgcaga gaaggcctac gactcttggg gcgagggtct tgatcttcat
      301 aaacaatcct cccgtttgac ggatatgcgc gagacagcac gtcttgtccc gttgtgaagt
      361 ttcgacgagc gcggtgtgaa tccctgcatt ttcgtttcag gtgtccatca gttcgccgat
      421 aatggggtgt acccgagcgt ctggagagcg agatgaagat gcgaaccaag atcctgccgt
      481 tggtgcttat cgcaacgttc gtcctcgggt caacagcagt ggctcgtgcg gatgatgaga
      541 cttcgagggg ggccggtcca tcgcccgagc ggtgcgccgc caagttggcg aaactagaga
      601 gttcattcga gaagcagacc gccaaacttc aggcgcagtt tgatcgcaag aaggcaaaac
      661 tcgctgagca acttgcaaag cacaccgatg accctgaagc tcaggcacgc ttcgagcgca
      721 agcaggccag gctacaggcg tggttcgaca agaagtcagg caaactcaat gcccggcacg
      781 atcgcaagaa ggctaagctc ggatccaagt gtggggcccc cgcaacggcg tagggaactt
      841 tccggcgcaa tcggtcgtcc aaaacgcaga ccccttatgt agggaggagt gcgtgaagag
      901 gatcgtgctg gttctggctc tgctgctggt tggcgcttcc tgcacacgaa cccccgacaa
      961 acacccacgc cccctgcccg tccaacaagg acagtccgac atctctgtcg ctggatcctg
     1021 cgtcgagacc tactctctgg agactttgaa ggatcgtgag atagcgttcg acggcgtcgt
     1081 cgcagaagtc gtcactaccg agtcaccatc aagcgtccag ggtgaggggc ccgaaactcg
     1141 atacgacgtt cgcttcgacg tgaacaggtg gttcaagggt ggatcgggcg atgcgacgac
     1201 cctgcggagc agtatcccgc taggcgatgg agatggcgcg gtcacctctg tcgatggcat
     1261 cgccatcact aaggggtcgc gctatctcgt gtcgggtgac ggcggattca tctggagctg
     1321 cggtttcacg agcgaatact ccgaggccga ggccgctagt tgggcgaata ctctgggggc
     1381 gggctagctg ggggcggtcg ctacggcgtt gatcgcaacc ccggtgagta gatacccatc
     1441 gccgtcacgg aaagcctcga tctcggcccg cacagcttcg cgaacctcga tcagctcggc
     1501 ttcatccata tcgcggagta gaagggcgat cgctcccgcg attgctcggc tgaaagacca
     1561 gtagtcgtcg aagtccgcga atctccactc agctgagacg ggctcgatct tccagtcgac
     1621 gaaacccgca tcgcccatca tgccttcgac gacggatggc tcggccatcg agaacatgcc
     1681 ccctggagct tgcggatcgg ggggcgtcag cttcccgaga ctcaccataa ctctgcccgc
     1741 gatgatggcc catatgttgt ctcgagccgc gccccagacc gagaaggcca acttcccacc
     1801 aggcttcagg acacgtcgga tctccttcag tgctgtctgc ggatccatca tcagcatgag
     1861 tccccagcga cagaggacgc cgtcgacgct cgcatctgga agatcgatcg cttccgcgtc
     1921 catgactcgg aactcggcgt tcgtgactcc gagtcgtgcc gctgtcttct cggcgacctt
     1981 caccatcgcg ggggcgaagt cggtggagat gagcttgccg tcgtcgccga ccagcttcgc
     2041 cgcgacgaac ccggtcatcc caggtcccgc tgcgagctca aggatggtct gaccgccctt
     2101 cggatcgacc ttctcgacca tccactcgcc gatcttgcga cctgcctccc agaggaagtc
     2161 tgacttcagc tcccatccgg cagacatctc atcccatatc gcccggctcg tctcccgggc
     2221 cgcatcaagg ctcaagattg ctcccctctt acgatgtctt acgatgccgg ttcgatgttc
     2281 tggttcaagc ggaagaagtt ctgcgggtcg tacttcttct tcaactcctg cagccttgcg
     2341 tagttgtccc catatgccga acgcacgatg gcgtccttgt ccgactcctc cttgcccgtg
     2401 aagttgagat aggcctggcc ggtggagaat cgtttcatgt cgctgaaaca gtcgcgcacc
     2461 caagagatga cctgtttctc ttcggcagga tcccaccagt ttccatcgat gctcaccatg
     2521 aatggcgcgc tgcgatcccc gaaggcggtg tccttggctc caactcttcc gaccgctccg
     2581 cccatgaacg ggat
//
LOCUS       DATDOZ010000253         2448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2326127, whole genome shotgun sequence.
ACCESSION   DATDOZ010000253 DATDOZ010000000
VERSION     DATDOZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326127"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1827
                     /locus_tag="VI541_05130"
     CDS             169..1827
                     /locus_tag="VI541_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF70320.1"
                     /translation="MDDFLQDRPHASNRFRTDLAFRRTLERLLGPETFEEVTPELDAM
                     GALAVGDLARLAEQAEAHPPQHVPFDAWGRRVDRLVVDPAWLRLVDIGMEAGLVAIAY
                     EGRFGDRDRILQFGLQHLFMPASATADCPMAMTDAAARVLLNEAPELSKRYVPLLTRR
                     QDAFTSGQWMTEKEGGSDVGRTSTIARPLGDGTFTLHGTKWFTSATTADIALALARPQ
                     GAEEGSRGLSLFLLELRNHDGSWNGISVRRLKDKLGTRALPTAELDLDGCIAVPVGEL
                     GRGVAKIAPMLNITRVHAAFGSSAGVGAVLSLARDYASRREAFGRMLRDLPAHRTWMA
                     TIAATYEAGLALSFRAAELLGASEHGSGDLIARTVIPLTKMALARQGVWACSELLESF
                     GGAGYLEDTGLPRALRDLHVQCIWEGTTSVMALDVLRALSREGAAVAFLDEVRDKVRT
                     FEHPLLSDVAAKVLAATDQLASLMTSADESSARRLAWGMARTYQAALLHHAAGWELDK
                     HGDATASAAARTFASQALIDEGSDLSMDETAELAFGRGLGEIDA"
     gene            1824..>2448
                     /locus_tag="VI541_05135"
     CDS             1824..>2448
                     /locus_tag="VI541_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLF70321.1"
                     /translation="MSEGAREGPFVISLDIGTSRIKGALVGRELSPFEDPVVRSTPSD
                     PDGLVEAVAEVLDILVSSCPRPPSAVAISCYWHGLLLVDRQSRPLGAITTWQDTRDGQ
                     GAQRLREIMDPAQYHAKTGCFIHPSYPMTRIFMSRFAAVSGDAGRMVGPAEWIIMEFF
                     GECVTSASMASGTGLTRIGGDEYDPEVLDAAGVGYAELPRLSESPLTG"
BASE COUNT          432 a          734 c          791 g          491 t
ORIGIN      
        1 atgggcaaca ctctcatcga ggctccagag ggcacgtgtc gcggaccagt cgtctcccca
       61 gaagtggttg cctgtcgagg tcgtatcggt ccgcatccgt ccaagactaa tcgctcgccg
      121 aacgctggag ttgaagttga ccctgtctcc gggtggataa tcattgtcat ggatgacttc
      181 ctgcaggatc gaccgcacgc atcgaatcgg tttcggaccg atctcgcctt tcgccgaacg
      241 cttgaacgtc tgctgggtcc agagacattc gaagaggtca cacccgaact cgatgccatg
      301 ggcgctctcg cggtagggga cctggcccga cttgccgagc aggccgaggc gcatccgcca
      361 cagcatgttc cgttcgatgc atggggtcgg cgagtggatc gcctcgtcgt ggacccggcc
      421 tggttgaggc tcgtcgatat cgggatggag gctggcctcg tcgctatcgc ctatgaaggt
      481 cgcttcggcg atagagatcg catcctccag ttcggattgc agcatctgtt catgcccgcg
      541 agtgctaccg ccgactgccc catggcgatg accgatgccg cggcaagggt cttgctgaat
      601 gaggcccccg aactctccaa acgctacgtc cctctcctga cccgacggca ggatgcgttc
      661 acatcgggtc agtggatgac cgagaaggag ggcgggtccg acgtcggtcg tacatcaacg
      721 atcgcgcgtc cactcggaga tggcaccttc actcttcacg gcacgaagtg gttcacctcc
      781 gcaactaccg cagatatcgc actggctctg gctcggccac agggggccga agaaggcagc
      841 cggggcctct cgctgttcct gctcgagcta cgaaaccacg atggttcttg gaacggcatc
      901 agtgtcagac gactcaagga caaactcggc acacgtgctc taccgaccgc ggagctcgat
      961 ctcgacggtt gcatagcggt tccggtcgga gagcttggcc gcggtgttgc caagatcgca
     1021 ccgatgctca acatcacccg tgttcacgcg gctttcgggt cgagcgctgg agtgggcgcg
     1081 gtcctttcat tggctcgcga ctacgcatct cgacgcgagg ccttcggtcg catgctgcgc
     1141 gatctgccag cgcaccgaac atggatggcg acgatcgcag cgacctacga ggcgggactc
     1201 gccctcagct tccgtgccgc ggaactcttg ggtgcgagcg agcatgggtc aggcgacctg
     1261 atcgcccgga cggttatccc tctcaccaag atggcacttg ctcgtcaagg ggtctgggca
     1321 tgcagcgaac tcctagagag tttcgggggt gccggttacc ttgaagatac ggggctaccg
     1381 cgtgctcttc gcgacctgca tgttcagtgc atctgggagg ggacgacctc ggtgatggcg
     1441 ctcgacgtgc tgagagcatt gagcagagag ggggccgcag ttgcgttcct cgatgaggtg
     1501 agagacaagg tgaggacctt cgaacatccc ctcctatcgg atgttgctgc aaaggtcctc
     1561 gctgcgaccg accaactcgc atctttgatg acctcggccg acgagtccag tgctcgtcga
     1621 cttgcatggg gaatggcgcg gacgtatcag gcggctctgc tgcatcacgc ggcgggatgg
     1681 gaactcgaca agcacggtga tgcgaccgct tctgccgctg ctcgtacctt cgcatcgcag
     1741 gcgctcatcg atgagggttc ggacctctcg atggatgaga ccgcggagct tgccttcggt
     1801 cgggggctgg gggagatcga cgcttgagtg agggggcccg cgagggtcct ttcgtcatca
     1861 gccttgatat aggcacatcg cggatcaagg gagcgctcgt cggtcgagag ctatccccgt
     1921 tcgaagaccc ggtcgtgcgc tcgacgccgt ctgatcccga cggtcttgtc gaggctgtgg
     1981 ccgaggttct cgacatcttg gtgagctcat gtcctcggcc cccaagcgct gttgcaatct
     2041 cgtgctattg gcatggtctg ctcttggtgg accggcagag ccgacctctc ggcgcgataa
     2101 ccacctggca ggacacgcgc gacgggcagg gggcccagcg tcttcgcgag atcatggacc
     2161 cagcgcagta ccacgccaag accggctgct tcatccatcc cagctatccg atgacccgga
     2221 tcttcatgtc tcgattcgct gcggtgagtg gtgatgccgg gcggatggtc ggtccggctg
     2281 aatggatcat catggagttt ttcggcgaat gcgtcacgag tgcctcgatg gcgtcaggga
     2341 cgggactgac tcgcatcggg ggcgacgagt acgaccctga agtactcgat gcggcggggg
     2401 tcggctatgc cgagttgccg aggctctccg agagcccgct aacgggac
//
LOCUS       DATDOZ010000254         4719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3907659, whole genome shotgun sequence.
ACCESSION   DATDOZ010000254 DATDOZ010000000
VERSION     DATDOZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907659"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2557)
                     /locus_tag="VI541_05140"
     CDS             complement(<1..2557)
                     /locus_tag="VI541_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF70322.1"
                     /translation="MKQLAINALLTLPLVGAYAMFAIGIVVIYRASRVLNLAHGAMAT
                     LPAYISYSLVEAGLGPHVAFLVAVGAGALLGIGVERFVLRRLRGESQTSQTVGTVAVL
                     GILIAIIAKIWGTAPLRAPSIFPSGSVQIGLTPMYYADIGIAVFALAAAGAFLLLFQR
                     TEIGLAMRGAAENGQAARLMGINPDRTTALAWALGGALAGASGILLAAATNLHPFTLS
                     RQVLPGFVAALIGGLGSLTGAMAGSVIVGMTQGIVSSVGPLASQPGSAQMVLALVAIG
                     VMAVRGRSLSGTSEVGAVHGVIPAMRVKRRRWLLWVPVLAGAGFLPFIPDSVVRDANV
                     AAIYVVVAVSLVLLTGWVGQISLAQASFVGIAAFTTGILVRDAGIPFPLNLPIAAAVS
                     GLAAAGLGVVALRVRGLYLAVATLIFGWMSDAYLFKSSWLVGAGGSSTITGRTIGRRG
                     AFPSFDLTQSRSFWLVALAAAVLALVAAANIRDSRSGRALFAVRGSELAAASMGIDVT
                     RMKLVAFALSGVLAGIAGNLLMVDQRTASPDQFSFSASLLFLSITAVGGLVRLGGVVA
                     ASVGFAALLELYFKVPAFQGYLDIVTSSLLLVAIMASPASARAKIASVMSRLIGPLRV
                     TMTDAISLGTAKMGSLLRLPRKAATHADPPVARAEPPAAPEGFPLKVDGVTVRFGGLT
                     AAEDVTLEVAQAEIVGLIGPNGAGKTTVFNAISGFVQPTEGGVHLFGHVVTKMPVHAR
                     ASLGLARTFQVLQLFHDATVFDNLLVATHLHSRVGLGSNIFLTAKSLRAEREARESVR
                     AIVDLVGIDEYVDAKAGDLPFGTLRRVDVARALVTGGRLMLLDEPASGLDERET"
     gene            complement(2582..4006)
                     /locus_tag="VI541_05145"
     CDS             complement(2582..4006)
                     /locus_tag="VI541_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF70323.1"
                     /translation="MRGRTILRSSLALAMVGVLSFLSAGPSFRDHVVERVRATGATAE
                     AAEAGGVEVSAGGAGERISTRSRAGQAVGGLECVRGRNGGATDVGVTGSSIKLAATVV
                     KTGVGSSFLGDVPIALEAVKNRVNRAGGVCGRLLELEMVDDRWDATEGLGDIRKFIAA
                     GKFALAVSPSSQGLDAAIRNGDISRAGIPVVGADGMLISQYTDPWVWPVAASTITTMH
                     VMAKNAAERGAKTFGLVYDQSYHFGVEGASGFRGALSRIFPGGNVLLSDIGVDAGQPS
                     YKNEVQRFNTECASCDFVAMLLEPPTALQWIRDGGDPGNMEQGGTGGPQPLLVNSFAR
                     ACGAPCAGMWVWTGYTPPIYPFDSIPEVTKFVNDIRAQSSGADVNNPFVQGGYLGMEL
                     LVEGLRQVGPNLTRARLKTVLDNITFDRGLSQPLTWRQGDHFANNSAQAFSIVVNAGS
                     FSSWRYEQTGWVVDPWPNLDAPKR"
     gene            complement(4003..>4719)
                     /locus_tag="VI541_05150"
     CDS             complement(4003..>4719)
                     /locus_tag="VI541_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70324.1"
                     /translation="GKVRLAELSTEAQTWAKGRRGSADSSFDRRIVGLQVDADGDGVF
                     EDVCDVCSVDAAREAMNRALIGVGSVDFPSPDGEYLDGSPGGFQAVVEKDRHQFYADR
                     ALNDDDSQAVVGLMFFLQSDARAGRSRQIVQIAGVQAESHYGIYLLPTAASSQREEVR
                     NDLLERVIERVPATPDPPAPPPSLPIKAKEIIDRFVERVAQGWRVFVAKPGDALLMAA
                     LLSFLLLPLYLVSRRRELGS"
BASE COUNT          978 a         1551 c         1430 g          760 t
ORIGIN      
        1 cggtctcgcg ctcatcgagg ccagaggcag gttcgtctag cagcatcagg cggcctcccg
       61 taaccagggc ccgcgcaaca tcgaccctgc gcagtgttcc gaacggcagg tctccggcct
      121 tcgcatccac gtattcatcg atacccacca ggtccacgat cgcacgaaca ctctcacgag
      181 cctcgcgctc tgcccgcagt gacttggcgg tgaggaagat gttcgagccc agcccgactc
      241 gcgagtggag gtgcgtcgca acgagaaggt tgtcgaagac cgtcgcgtca tggaagagct
      301 ggagcacctg gaaggttcgg gccagaccaa gcgatgcgcg cgcgtgaacg ggcatcttcg
      361 tgactacatg cccgaagagg tgcaccccgc cttcggtcgg ctggacgaaa cctgagatcg
      421 cgttgaagac cgtagtcttg cccgcaccgt tcgggccgat caggcccacg atctcggcct
      481 gagccacttc gagagtcaca tcctcggctg ccgtcaatcc gccgaatcgg acggtaaccc
      541 cgtcgacctt gaggggaaac ccttcgggcg cggctggcgg ctcagcgcgc gcgaccgggg
      601 gatctgcatg ggtagccgcc tttctcggga gtcgcaggag gctacccatc ttcgcggtac
      661 ccaaagagat ggcgtcggtc atcgtcaccc tcagggggcc gatgagacga ctcatcactg
      721 aagcgatctt cgctcgcgcc gacgccggcg aggccatgat cgcgacgaga agaagactcg
      781 atgtcacgat gtcgagatat ccctggaacg cggggacctt gaagtacagc tcgaggagtg
      841 ctgcgaatcc gaccgaggca gcgactacac cacccaggcg caccaacccg ccgaccgcgg
      901 tgatcgagag gaagagcagg ctcgcgctga atgagaactg gtcgggggat gcggtccgtt
      961 gatccaccat gagcagattg ccagctatcc ccgccaacac ccccgatagt gcaaatgcga
     1021 caagcttcat acgcgtgaca tcgatgccca tcgatgccgc cgcaagctcg gaccccctga
     1081 cagcgaagag cgctcgccct gacctcgagt ctcggatgtt ggccgcagcc accagagcaa
     1141 gcaccgcagc agccagtgcg acgagccaga aactgcggct ctgcgtgagg tcgaatgagg
     1201 gaaaggcccc acgtcggccg atggtccgtc ccgtgatggt cgatgatccg cccgcgccaa
     1261 cgagccacga ggacttgaag agataagcat ccgacatcca cccgaagatg agtgtcgcga
     1321 ctgcgaggta gagaccgcgc acgcgaagag cgacgacccc gagacctgcc gccgccaacc
     1381 ccgacaccgc agcggcgatc ggcaggttca gcgggaatgg gatgcccgca tcacgaacaa
     1441 ggatcccggt cgtgaacgct gcgatcccca cgaaactggc ctgtgccaga gatatctgtc
     1501 cgacccaacc ggtcaacaac acaagtgaaa cggcgacgac tacatatatg gctgctacgt
     1561 tcgcgtctcg cacgaccgag tctgggatga agggaaggaa tccggccccc gcgagaactg
     1621 ggacccatag gagccatcgc cttcgcttca cccgcatcgc agggatcact ccatggacag
     1681 ccccgacctc ggatgttccc gagaggctgc gccctcggac ggccatgacg ccgatggcga
     1741 cgagcgcaag gaccatctgg gccgagccgg gctgactcgc tagcggtcca acagaggaga
     1801 cgatcccctg agtcatcccg actatgaccg aaccggccat cgctccggtg aggctcccaa
     1861 ggccgccgat cagagccgcg acgaaaccgg gcagcacctg ccgagagagg gtgaacggat
     1921 gcagattcgt cgccgctgcg agaagtatcc ccgaggcccc cgcaagcgcg cccccgagag
     1981 cccacgcgag tgcggtggtc cggtcggggt tgatccccat cagacgcgcc gcctgcccgt
     2041 tctcggcagc acctctcatg gccaagccga tctcggttcg ttggaacaga aggaggaatg
     2101 ctcccgcggc agcaagagcg aagaccgcga tcccgatatc ggcgtaatac atcggggtca
     2161 acccgatctg cacgctgccg gatgggaaga tcgatggggc acgaagtgga gcggtccccc
     2221 agatcttggc gatgatcgcg atcaggatgc caagcaccgc aacggttccg accgtctgcg
     2281 aggtctggga ctcgccgcga agtcgacgca gtacgaacct ctctactccg atgccgagca
     2341 gcgcgccggc gccgacggcg accaggaacg cgacatgggg gccgagcccc gcttcgacca
     2401 acgagtaaga gatgtaggcc ggaagagtcg ccatggcgcc gtgggccaga ttcaggacac
     2461 gcgatgcccg atagatcacc actatgccga tggcgaacat ggcatatgcg cccaccaggg
     2521 gcagggtcaa cagagcgttg atggccaact gcttcatgca gggcgagtat gcgtcatgcg
     2581 ttcagcgttt tggggcgtcc aggttcggcc aggggtccac tacccaaccg gtctgttcgt
     2641 acctccacga cgagaacgac cctgcattga cgactatcga gaacgcttgg gccgagttgt
     2701 tcgcgaagtg gtcaccttgg cgccatgtga gcggttggga gaggccgcga tcgaaggtga
     2761 tgttgtccag caccgtcttg aggcgggcac gcgtgaggtt ggggccgacc tgacgcaatc
     2821 cttcgaccag aagttccatc ccgagatagc cgccctgcac gaaggggttg ttcacgtcag
     2881 caccggaact ctgggcacgg atgtcgttga cgaacttcgt cacctccggg atagagtcga
     2941 acgggtagat ggggggcgta taaccggtcc agacccacat ccccgcgcag ggggcgccgc
     3001 acgctcgggc gaatgaattg accaacagag gctgcggccc cccagtgccc ccctgttcca
     3061 tgtttccggg gtctcccccg tcgcggatcc actgaagcgc tgtcgggggc tcgaggagca
     3121 tcgcgacgaa atcacatgac gcgcattcgg tgttgaacct ctggacctcg ttcttgtagc
     3181 tgggttgccc ggcatcgaca ccgatgtccg agagcaggac attcccaccg gggaagatcc
     3241 gtgagagtgc tcccctgaaa cccgaggccc cctcaacacc gaagtggtag ctctggtcgt
     3301 agacgagacc gaaagtcttg gctcctcgct ctgccgcgtt cttcgccatg acgtgcatcg
     3361 tcgtgatcgt cgaggcagcg acaggccaca cccaggggtc ggtgtactgg ctgatcaaca
     3421 tcccgtctgc tcccacgacc gggatcccgg cgcgagagat atcgccgttg cggatggctg
     3481 cgtcgagacc ttgagatgaa ggcgaaaccg ccaacgcgaa tttccccgct gcgatgaact
     3541 tccggatgtc gcctagacct tctgtcgcat cccatctgtc gtccaccatc tcgagttcca
     3601 gcaaccgccc gcacacgccg cccgctcgat tgacgcgatt cttcacagct tcgagcgcga
     3661 tcgggacatc tccaaggaaa cttgaaccca ctccggtctt gacaaccgtc gccgcgagct
     3721 tgatcgatga acccgtgacc ccaacatctg ttgcgccccc gttgcggcct cggacacact
     3781 ccagtcctcc cacagcctga ccagcacggc ttcgagtgct gatccgttcc ccggccccgc
     3841 ccgcgctgac ctcgacaccg cccgcctcgg cggcctcagc cgtagcaccg gtcgcccgga
     3901 cacgctcaac gacatggtct ctgaacgagg gccctgcaga gaggaatgag agaacaccaa
     3961 ccatcgccag agcaaggctc gatctgagga tcgtccggcc cctcatgatc ccaactccct
     4021 tcggcgagag acgagataga gaggcagcag aagaaacgag agaagagctg ccatcaagag
     4081 tgcgtcaccc ggtttggcga cgaacacgcg ccatccctga gcgactcgct ccacgaacct
     4141 gtcgatgatc tccttcgctt tgataggcag tgacggcggg ggagctgggg gatctggagt
     4201 tgcgggcaca cgttcgatca cccgctccag caagtcgttg cgaacctctt cccgttgaga
     4261 agaagcagcc gtcggcagga ggtagatacc gtagtgagac tcggcctgaa caccggcgat
     4321 ctgcacgatc tgccgcgaac gacccgcacg cgcgtcgctc tgcaagaaga acatgagccc
     4381 cacaacagcc tgcgagtcgt cgtcgttcaa tgctcggtcg gcgtagaact ggtgccgatc
     4441 cttctcgacc acggcttgga aacctcctgg ggacccatcg aggtactcgc catccggcga
     4501 ggggaagtca accgaaccca ctccgatcag cgctcggttc atggcttcgc gcgcggcgtc
     4561 gacactgcag acatcgcaga cgtcctcgaa gactccgtca ccatcggcat ccacctgcaa
     4621 tccgacgatc cgacgatcga aggaggagtc agccgaaccc cgccgacctt tcgcccaggt
     4681 ctgcgcctcg gtgcttagct cggccaaccg aaccttccc
//
LOCUS       DATDOZ010000255         4102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_213454, whole genome shotgun sequence.
ACCESSION   DATDOZ010000255 DATDOZ010000000
VERSION     DATDOZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213454"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..609)
                     /locus_tag="VI541_05155"
     CDS             complement(22..609)
                     /locus_tag="VI541_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70325.1"
                     /translation="MKRRTFDLLMSIGGLVITLVMVIAGVLLFWGYNFANESVTQQLS
                     AQKIFFPEKGSEALAPPEIGPYLNRYAGQQLVTGEQALAYSDHFIAVHLKAVAGGKTY
                     SEASTAARANPNDPKLASQVQTLFRGETLRGLLLNAYAFWKIGQLARLGSIAAFSVGG
                     IMLILTGLGFWHSRRASPADEVLADKIRTRTVTAA"
     gene            complement(736..963)
                     /locus_tag="VI541_05160"
     CDS             complement(736..963)
                     /locus_tag="VI541_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70326.1"
                     /translation="MELTSEQFEKLKTAFDRVQMARRELDSLIGDNYLSPRVNIVGIH
                     FAPLAAAVEKLRWAEKDWRELMEEISTAANA"
     gene            complement(993..1814)
                     /locus_tag="VI541_05165"
     CDS             complement(993..1814)
                     /locus_tag="VI541_05165"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLF70327.1"
                     /translation="MTLLQSIVLGLVQGLTEFIPISSKTHLIIIPALFHWKEPPLAFD
                     VILHGGTLLAALVYFRRDLLEILEGIDRPGPARKVLLLLAIGAIPAGIAGALLESRLS
                     VLYRRPALAAALLVITGSILAITETYFRRHPHSESIEDTTFTNIEKISAEVTPGKAAF
                     IGAAQIFALSPGISRAGTTIGAGLLARLSRAQAARFSFLISIPLIAGATLAEIPKLGQ
                     AHLGTAVILAGLVSSFVAGYVAIAGMIGYLQRRGLYPFAAYCFVVGPIVAFALAR"
     gene            complement(1811..2551)
                     /gene="cpaB"
                     /locus_tag="VI541_05170"
     CDS             complement(1811..2551)
                     /gene="cpaB"
                     /locus_tag="VI541_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HLF70328.1"
                     /translation="MSSYILARRRTTLTGAMAVIAALVTGLAIYSYLSFVRAQIPLAG
                     KLVPLVVAATDIEPGTTMEASLVKVVTHPQRYLPPLALHSIESVLGKVTTVPIFAGEP
                     LTARRFGVTGGRSSSIPKGLRAFTLTFESAGAMGTIPGVGDRVDVIATFTREVMGEAS
                     SVTILRQKEVAEVVGGGGPSSAKVGSRLALPGSAQASIGVTLFVTPTEAQKLAMAQSL
                     GRLTLVLAPVGDDEAAPPPMSPSDLAAS"
     gene            complement(2614..2973)
                     /locus_tag="VI541_05175"
     CDS             complement(2614..2973)
                     /locus_tag="VI541_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007621057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HLF70329.1"
                     /translation="MPTYEYACTVCGDHFDRVQSFVEDPLTVCDICGGRLRRVFHAAG
                     LLFKGSGFYSTDSKSGAKPAARANGSRDSKGASTGDSGDRSTSASISESKTGSTGESK
                     GESKGSRSSPAPAKENS"
     gene            3138..>4102
                     /locus_tag="VI541_05180"
     CDS             3138..>4102
                     /locus_tag="VI541_05180"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HLF70330.1"
                     /translation="MSLQSRLFAFFIVIVVMPLTIAPLIGQRILVRELERRTFEQIKA
                     AESAEMKVFDEKRSRANERVAVVARDPALVAAIRARRSAEADQVLAGHRQEVNDPLSF
                     IVVTDTSGRVVASSLTEPSFVPAVTLPIVDDLARSSAPDQRLLFYRDEAYVTESGPVP
                     DSPVTLGRVYGGFYYDERFLSSLSTQSSVGTTLFVAGRAVASTLRTVKTSKAAVTITP
                     LLYSSEGKRLSLKISGVPSYAIARPLNHGVRISEASFVFSRPQSSVIAVKRTLQRSLF
                     GLLGAAILIAGTLGWALARVISRPLRELAAGANQIAIGNYDQTIRV"
BASE COUNT          756 a         1219 c         1312 g          815 t
ORIGIN      
        1 cgttttcttc cgggtgtctg gctacgcggc ggtgacagtc ctggttcgga tcttgtccgc
       61 caggacctcg tcggcgggag aggcgcgccg tgagtgccag aacccgagtc cggtcaggat
      121 cagcatgatt cccccaaccg agaacgcagc gatgctgccc aaccttgcaa gctgcccgat
      181 cttccagaat gcataggcgt tgaggagaag tcccctgaga gtctcgcctc ggaagagagt
      241 ctggacctgc gaggcaagtt tgggatcgtt ggggttagcc ctcgcagcgg tactggcctc
      301 cgagtaggtc ttgcctcccg ccacggcctt gaggtgcacg gcgatgaagt ggtcggagta
      361 tgcaagagcc tgctcgcccg taacgagctg ctggcccgcg tatctgttga gatacgggcc
      421 gatctcgggt ggagccaagg cttcacttcc cttctctggg aagaagatct tctgggccga
      481 gagctgctgg gtcacgctct cgtttgcaaa gttgtaaccc caaaacagga gaacccctgc
      541 gatcaccatg accagggtga tcaccagccc tccgatggac atcagcaggt cgaacgtccg
      601 cctcttcatg acttcctccc tttccacgag ttgtccgttc cctacggtaa gtatcgggag
      661 cgtggagagg ggtcgatagg gccataggcc cccccactct gggcccgatg gccctatttc
      721 atgcggtttg ggggctcagg cgttggccgc tgtggagatc tcctccatca gctcgcgcca
      781 atccttctcg gcccagcgca gtttctcgac cgctgctgcc agaggggcga agtggatccc
      841 cacgatgttg actcggggcg agaggtagtt gtcgccgatc agtgagtcga gttctcgccg
      901 tgccatctgg acgcggtcga aagcggtctt aagtttctcg aactgctcgg acgtcagttc
      961 catgatcagg cccccaaagg tatggccgga ttctatcggg ccagggcgaa ggcgactatg
     1021 ggccccacga cgaagcagta tgcagcgaac gggtagaggc cgcgtcgctg tagataaccg
     1081 atcatccccg cgatcgcgac gtagccggcg acgaacgatg agaccagacc ggccaggatc
     1141 accgctgttc ccagatgagc ctgcccgagc tttgggatct cggccaatgt tgcccccgcg
     1201 atcaatggga tcgagatcag gaacgagaac cgcgctgcct gcgctcggga tagacgtgcg
     1261 agcaggcccg ccccgatggt ggttccagcc ctcgatatcc cgggcgataa ggcgaagatc
     1321 tgggccgccc cgatgaaggc ggccttccca ggtgtgacct ccgccgagat cttctcgatg
     1381 ttcgtgaaag tcgtgtcttc gatggactct gagtgcggat gtctgcggaa gtaggtttct
     1441 gtgatcgcta gtatcgatcc tgtgatcact aacaacgccg cggctagtgc cggtcttcga
     1501 tagaggaccg atagccggga ttcgaggaga gccccggcta tcccagcggg gatcgcccct
     1561 attgccagaa gaagcagcac ctttctggct gggcccggcc tgtcgatacc ttcgaggatc
     1621 tcgagtaggt ccctgcggaa gtagacgagt gctgcgagca gggttcctcc atggagtatc
     1681 acgtcgaatg ctaagggtgg ctccttccag tggaatagcg cggggatgat gatgaggtgc
     1741 gtcttggatg agatgggtat gaactcagtt aagccttgta ccaatccgag cactatggat
     1801 tgaagcaggg tcaactggct gccaggtctg aaggggacat cgggggcggg gccgcttcat
     1861 cgtcgccgac gggagcgagc actagagtaa ggcgacccag cgactgtgcc atcgcaagtt
     1921 tctgagcttc ggttggcgtg acgaacaaag tgactccgat cgatgcctgt gccgagccgg
     1981 ggagcgcaag gcgcgagcca accttagccg aggacggtcc tccgccaccg acaacctcgg
     2041 caacttcttt ctgacgaagg atcgttactg aactggcttc gcccatgacc tcacgagtga
     2101 atgttgcgat cacgtctacg cggtcgccga cgccggggat ggttcccatc gcaccggcgg
     2161 attcgaacgt aagcgtgaat gctctaagcc ccttagggat gctcgatgac ctcccaccgg
     2221 tcacgccgaa tctgcgagca gtgaggggct cgccagcgaa gatcgggacg gtcgtgactt
     2281 tccctaggac gctctctatc gaatgcaggg ccaggggtgg aaggtatcgc tgcgggtgag
     2341 tcacgacctt gaccaaggac gcttccatcg tcgtgcccgg ctcgatgtcc gtcgcagcga
     2401 ccaccagtgg gaccagtttg ccggcgagag ggatctgggc gcggacgaat gatagatacg
     2461 agtagatggc aagaccggtg acgagggccg cgatgaccgc catcgcaccg gtgagagtgg
     2521 ttcttcggcg ggccaggatg taactgctca ttgctgcctc cctccgggcg ggggggaagt
     2581 gttggaatcc tagcgcgacg tgcgctccgg gtgttaggag ttttccttcg cgggggccgg
     2641 ggaggatcgg gaccctttcg actcgccctt cgactctcca gtggaccccg tcttcgactc
     2701 gctgatcgat gccgaagtgg acctatcgcc cgagtcgccc gtcgaggcac ccttcgagtc
     2761 gcgtgagcca ttggccctgg ccgctggttt ggctcccgac ttcgagtcgg tggagtagaa
     2821 ccctgagccc ttgaagagca gtcctgcagc gtggaagacg cgccgcaagc gtcccccgca
     2881 gatatcgcag acagtaaggg ggtcctccac gaatgactgc acccggtcga agtggtcgcc
     2941 gcagacggtg caggcgtact cgtaggtggg cactcgactc cttccgatgg gtccccgtgg
     3001 gaacggggac gatccttcac aacttagtat gccctgtggt gagggtgggg gcccgatcgg
     3061 gggatccgac tcaagcaatc tgtccaagtt tccgaccatt taggggcggg cccagggggg
     3121 cccctggggg gagtggcgtg tcgctacaga gcaggctctt cgccttcttc atagtcatcg
     3181 tggtgatgcc cctgacgatc gcgcccctta tcggtcagcg gatcctcgta cgcgagctcg
     3241 aacgacggac attcgagcag atcaaggcgg ccgagtctgc cgagatgaag gtctttgatg
     3301 agaagcgttc gcgcgccaac gagcgcgtcg cggtcgtggc acgtgacccc gctctcgtgg
     3361 ctgcgatcag ggcccgccgt agcgctgagg cggatcaggt tctcgcaggt catcgacagg
     3421 aggtaaacga ccctctctcc ttcatcgttg tcaccgacac atcgggcaga gtcgtcgcta
     3481 gctctctgac cgaaccatcc ttcgtacccg cagtcaccct gccgatcgtg gatgatctgg
     3541 cgaggtcctc ggcacccgat cagaggctcc tcttctatcg cgacgaggct tacgtaactg
     3601 agtccgggcc tgtgccggac tcgcctgtga ccctcgggag ggtatacgga ggtttctact
     3661 acgacgagag gttcctctcg tctctctcga cccagtcatc ggtcggcaca accctcttcg
     3721 ttgccgggcg ggcggtcgcc agtaccttgc ggaccgtcaa gacatcgaag gctgccgtga
     3781 ccataacgcc tctgctgtac tcatctgagg ggaagcggct cagcctgaag atatctggag
     3841 tgccgagcta tgcgatcgcg aggcccttga accacggcgt gaggatctca gaggcctcct
     3901 tcgtcttcag ccgcccccag agcagcgtga tcgcagtcaa aaggacccta cagcggtcgc
     3961 tcttcggact actcggtgcc gccatcctca tcgcgggtac gctcggctgg gcgttggcgc
     4021 gggtgatctc gcggcccctg cgtgaactgg cggccggtgc caaccagatc gccatcggga
     4081 actacgatca gacgatacgg gt
//
LOCUS       DATDOZ010000256         3804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3377818, whole genome shotgun sequence.
ACCESSION   DATDOZ010000256 DATDOZ010000000
VERSION     DATDOZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377818"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="hisD"
                     /locus_tag="VI541_05185"
     CDS             complement(<1..1042)
                     /gene="hisD"
                     /locus_tag="VI541_05185"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLF70331.1"
                     /translation="MERLDLRGSPDLSITRPPVASEPIETVRNIIEDVRTRGDRALRE
                     MTLRFDGVAVEELRVPGGVIDASLRETPAPIREALELAAANIRRFATAQLVHPWSETI
                     DGATVGEAVTPVGRAGAYVPGGRATYPSTVLMSAIPASVAGVVELALCVPPGPDGEVP
                     SVTLAAAAIAGVSEVYRVGGAQAIAAMAFGTESIPKVDVIVGPGNIYVALAKREVSGY
                     VGIDSVAGPSEIAIVADGTADPTAIAYDLIAQAEHGPDGSFVLVTWDEALASAVDGAL
                     NRSFTEIGAGDALRTALERGSVVALVQDLSQAITVVDRFAPEHLELIFDGAQDAAGSF
                     RNAGAIFVGPYSP"
     gene            complement(1023..1556)
                     /locus_tag="VI541_05190"
     CDS             complement(1023..1556)
                     /locus_tag="VI541_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70332.1"
                     /translation="MNGGGDDVSPASRTLLNLLRLGFVLLGLGSVGIGIWMFAQPVGW
                     SDVFPAGISDLGPINPHFIRDVGGWLVAGGTLYLFASLKPLRFGGVALLVNLIGSSLH
                     AAGHVMDLTSGSIPAKHWIVDFPGVFLPLLIQGILVWMLWAVTSGRYESHGETADLAD
                     DGAEHPIDSQRYGETRP"
     gene            complement(1611..1814)
                     /locus_tag="VI541_05195"
     CDS             complement(1611..1814)
                     /locus_tag="VI541_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70333.1"
                     /translation="MKGKSAWARFVLKGEQVLMCPVCQGEVPSWTTMGDRCPNCSSPK
                     LVVVLDSVVCKSCGSDFERAAFL"
     gene            complement(1902..>3804)
                     /gene="dnaE"
                     /locus_tag="VI541_05200"
     CDS             complement(1902..>3804)
                     /gene="dnaE"
                     /locus_tag="VI541_05200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLF70334.1"
                     /translation="HEPLTNHVPVQRTDGEIVTQYEMTALEKIGLLKMDFLGLRNLTV
                     IGDALEHILRERGEQIDIDAIPLDDPTTFDLLKRANSAGIFQMESPGMTRLMRQLRID
                     RFEEIAALIALYRPGPMDEIPRYVKGKHNPDSVTYFHPLLQTVLADTNGVIVYQEQVM
                     EMFQVVAGFSAQEADMVRAAIGKKKSDQLVKWKDQFEKGSSASGLTPAEAQGLWELIL
                     PFAGYSFNRAHAHGYALVAYQTAWLKANYPVEYMAALLTSVKDDKDLVPFYLVECRSL
                     GIKVLPPNVNDSDLEFTPRSSSVLFGLSAIRNVGEQVASRVIEARNSGPFKSFEDFCK
                     RVDPSCLNKKVVESLAKAGALDCVGVTRSAVLQIDPKGEGGLVLSDQAAGLIETVMTE
                     RKSEDAGQFSLFAGAAQAGPVAEVIHDELTGIELSKALLLAAEKEMLGFYVSEHPLEA
                     VESALRYQTQAEVLDLREGPDHAVKTVGGVLAGMVRKFTKKGDIWYSGYLEDMKGSVE
                     IVVWPKVVEGTPPDLLMDDQIVTIKGRIELRDESVKLIAIEMRKPDLSGAMSPLRIRM
                     PAAGCTPDRLERLKGVLASHPGPAPVLLHLEAAARTTVLKLGGFSVELRTGLFAELKA
                     VLGPEAVFA"
BASE COUNT          757 a         1207 c         1131 g          709 t
ORIGIN      
        1 ccggcgagta ggggccgacg aagatagcgc cggcatttcg gaatgaaccc gctgcatcct
       61 gcgcaccgtc gaagatcaat tccagatgct cgggcgcgaa gcgatcgacc acggttatcg
      121 cctggctgag atcctgcacc aacgccacca cagagccgcg ctcgagggcc gttctcagtg
      181 catccccagc gccgatctcg gtgaaggatc tgttgagcgc gccgtcaacg gccgatgcca
      241 atgcttcgtc ccatgtcacg aggacgaaac tgccatcggg cccatgctcg gcttgggcga
      301 tgaggtcata tgcgatcgct gttggatcgg ctgtgccgtc tgccacgatc gcgatctcgc
      361 tgggtccggc aaccgagtcg atacccacat agccggatac ctctctcttg gccaacgcga
      421 cgtagatgtt gcccggaccc acgatgacat ccaccttggg tatcgactcg gttccaaaag
      481 ccatcgcggc gatcgcctgc gcaccgccga cgcgatagac ctcgctcact cccgctatgg
      541 ctgctgcggc gagggttaca gacgggacct caccatcggg ccccggcggg acgcagaggg
      601 caagttcaac aacacctgcg accgatgctg gtatcgccga catcaacacg gtcgacgggt
      661 aggtcgccct accacccggt acgtaggccc ccgccctccc aacaggcgtg accgcctcgc
      721 cgaccgtcgc tccatcgatg gtctcgctcc aggggtggac gagttgcgca gtggcgaaac
      781 gtcggatgtt tgcggctgcc agctcgagag cctcgcggat gggggccggc gtttcgcgaa
      841 gagatgcgtc gataacgcct ccgggtacac gaagctcttc gaccgccact ccgtcgaaac
      901 gaagtgtcat ctcgcgcagg gctcggtcac cgcgggttcg gacatcttcg atgatgttgc
      961 gaaccgtctc gatcggttct gacgcaacgg ggggccgcgt tatcgaaagg tcagggctac
     1021 ccctaaggtc gagtctctcc atagcgctga gagtctatgg ggtgttccgc tccatcatcg
     1081 gcaagatccg ccgtttcacc gtgactctca tatcgaccgg acgtaaccgc ccagagcatc
     1141 caaacgagga tcccttggat cagcagcggc aggaagaccc ccggaaagtc gacgatccag
     1201 tgtttcgcag ggatcgagcc tgaggttagg tccatcacat gaccagcagc atgcagtgat
     1261 gaaccgatca ggttgaccag cagggccaca ccaccgaacc tcaaaggctt gaggctggcg
     1321 aagaggtaga gcgtcccacc ggccaccagc cacccaccca catcgcggat gaagtggggg
     1381 ttgatggggc cgaggtctga gataccggca gggaagacat cgctccaacc caccggttga
     1441 gcgaacatcc agataccgat gcccacactg ccgagcccga gcagaacgaa ccccaatcgg
     1501 agcagattca gcagggttct gctagctggc gagacatcgt cgcctccacc attcatggct
     1561 cgatgataag ggcccgctcg gccacataac cggcaatgcc ccgcacgtct ttagaggaag
     1621 gcggcacgct cgaagtcgct cccacatgac ttgcagacca cggagtccaa gacaaccacg
     1681 agcttggggc tggagcagtt aggacagcgg tcgcccatcg ttgtccatga ggggacctca
     1741 ccctgacaca ccgggcacat gaggacctgc tcccccttga ggacgaagcg ggcccaggcc
     1801 gactttccct tcatgggatc cgtctgacgc cgatcgcacc tgaagcaggc catggtgccc
     1861 tctctggtag agagtgattc tacttcagcg gaaatgttgg atcaggcgaa aacggcttcg
     1921 ggcccgagga ctgccttcaa ctcggcgaag agccccgttc gaagttccac cgagaaacca
     1981 ccaagtttga gaacggtggt tcgggccgcc gcttcgaggt gcagaagcac gggggccggt
     2041 cctggatgcg atgcaagaac acccttcaat ctctcaagac ggtcgggcgt acagccagca
     2101 gcgggcatgc gtatccgaag cgggctcatc gcgcccgaga gatcgggctt tcgcatctcg
     2161 atcgcgatca acttcacgga ctcgtctctg agttcgatgc ggcccttgat cgtgacgatc
     2221 tgatcgtcca tcagcaggtc cggtggcgta ccttcgacga ccttgggcca gacgacgatc
     2281 tcgaccgaac ccttcatgtc ctcgaggtaa cccgagtacc agatgtcgcc cttcttcgtg
     2341 aacttgcgca ccatcccggc caggacccct ccgacggtct tgaccgcatg atcgggccct
     2401 tcgcgcaggt cgagaacctc ggcctgcgtc tgatagcgaa gtgctgactc gacggcttcg
     2461 agtggatgct ccgagacgta gaaaccgagc atctccttct cggccgccag tagcaacgcc
     2521 ttcgataact cgatgcctgt caactcgtcg tggataacct ctgccaccgg gcccgcctgc
     2581 gcggcccccg cgaagagcga gaactggccg gcatcctcgg acttgcgctc ggtcatcacg
     2641 gtctcgatca gtccggccgc ctgatcgctc agaacgagcc cgccctcgcc tttcggatcg
     2701 atctgcaaca cagcactgcg cgtcacgccg acacagtcga gggcccccgc cttggctagg
     2761 ctctcgacca ccttcttgtt gaggcaactt gggtcgacgc gcttgcagaa gtcttcgaag
     2821 gacttgaacg gcccactgtt gcgagcttcg atgacgcggc tggccacctg ctcgcccacg
     2881 ttgcggatcg ccgagagccc gaatagaacc gacgagcttc gcggcgtgaa ttcgaggtct
     2941 gaatcgttga cgttgggggg caataccttg atgccgagcg aacggcactc gaccagatag
     3001 aaggggacaa ggtccttgtc gtccttgaca gacgtgagca gcgcggccat gtactcgacc
     3061 ggatagttcg ccttcagcca cgccgtctga tacgccacca gtgcgtatcc atgcgcatgg
     3121 gctctgttga aggagtaacc cgcgaatggc aggatcaact cccaaaggcc ctgggcctcg
     3181 gcaggggtga gccccgacgc cgaagaacct ttctcgaact gatccttcca cttcaccaac
     3241 tggtcgctct tcttcttacc gatggcagca cggaccatgt cggcctcttg agcggagaag
     3301 cccgctacca cctggaacat ctccatcacc tgttcttgat agacgatcac accgttggta
     3361 tcggcgagga ccgtctgcag tagcgggtgg aagtaggtca ccgagtcggg gttgtgcttg
     3421 ccctttacgt atcgagggat ctcgtccatc gggccgggtc gatagagcgc gatcaaggcg
     3481 gcgatctctt cgaagcggtc gatgcgcagc tgccgcatca gtcgcgtcat ccccggcgac
     3541 tccatctgga atatgccagc agagttggca cgcttcaata gatcgaaggt ggtcgggtca
     3601 tcgagtggga tcgcgtcgat gtcgatctgc tcgccgcgct cgcgcaggat gtgttcgaga
     3661 gcgtcgccga tgacggtgag gttgcgcaga cctaggaagt ccatcttcaa cagaccgatc
     3721 ttctcgagcg cggtcatctc gtactgcgtg acgatctcgc catcggtcct ctggacgggc
     3781 acgtggttgg tcagcggttc gtgg
//
LOCUS       DATDOZ010000257         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3911488, whole genome shotgun sequence.
ACCESSION   DATDOZ010000257 DATDOZ010000000
VERSION     DATDOZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911488"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VI541_05205"
     CDS             <1..379
                     /locus_tag="VI541_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70335.1"
                     /translation="ADAPGAPPLSTMPATCTGSLGHLLPDFTEIVDTVDIFRSPQRCT
                     SPDCKVRATLTTAPGLRAEIGIEQIPGIFLTSSCSQSGCTASIDAPSLSYPNTDYPRI
                     RVRQISGRGSYALTVQRMSLAQL"
     gene            complement(387..1442)
                     /locus_tag="VI541_05210"
     CDS             complement(387..1442)
                     /locus_tag="VI541_05210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF70336.1"
                     /translation="MSKVETTYQIELFDPQKADRAAWERHHAFRRAVHALVRPDDPVD
                     PDELEETRMKREDPFGLRHFYSVTEGDRAIGFFGWGVMKPEAPGYESNKEYIWGGGEV
                     LPEARRRGIGREGLTLLRDDMIRYERTLATLWGVEEEDGHAALRHVGAEEKTVFAENR
                     LDLRKLDWDMVESWIAQGRAKSEDLELKLFEDELPEEFFEEYAPILSDLLMTMPFDDL
                     EHGDIVVTPETLKDDRDRMKELGAKHHTLIARKGGRLAGMTDVKWSPAKPAYIDQRFT
                     GVHPEFRGRGLGKLLKAYMLDFLRTRYDGLVWVITGNAHSNDPMLAINHALGFTEYKP
                     SSAYQIDLEALNKYLES"
     gene            1566..>2241
                     /locus_tag="VI541_05215"
     CDS             1566..>2241
                     /locus_tag="VI541_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70337.1"
                     /translation="MRRSVTAIAGMALVTSAFIVAQPGAQALPPLIANDNFADATVIT
                     SIPFTEATSNALATIETDEPQACGSLTKTLWYRYTPTSDGIISLGSTGGMTTSVFLGT
                     TLADLRTAACSEWGAYLPVFAGTTYHVQIGADSASTSFTVSANAVSGIAGRVTETSGA
                     PVRNYCIQLYDADGSWDDASYTSDRSSYQHQVAEEYEGMYIFFDPTPGTKKIEFGCGE
                     GLFEDEW"
BASE COUNT          450 a          695 c          632 g          464 t
ORIGIN      
        1 ggccgatgcg ccgggggccc ctccactctc gacgatgcca gcgacctgca cggggagcct
       61 cggtcacctg ttaccggact tcaccgagat cgtcgacact gtcgacatct tcagatcacc
      121 gcagcgctgc accagcccgg actgcaaggt cagagcgaca ttgaccacag caccaggatt
      181 acgggccgag atcggcatcg aacagatccc cgggatattt ctgacttctt cgtgcagcca
      241 atcgggctgc acggcttcaa tcgacgcgcc atcgctgagt tatcccaaca ccgactaccc
      301 tcgcataagg gttcgccaga tcagcggcag aggctcatat gcgttgacgg ttcagaggat
      361 gagcctcgcg cagctctgag gctgccttag ctttccaggt acttgttcaa cgcttcaagg
      421 tcgatctgat aagcgctgga tggcttgtac tccgtgaatc cgagggcgtg gttgatcgcg
      481 agcatcgggt cattcgagtg cgcgttgcct gtgatgaccc aaactaatcc gtcgtaccgg
      541 gtgcgcagga agtcgagcat gtaggccttg agcagtttcc ccagccctcg gccgcggaac
      601 tcgggatgca cacccgtgaa gcgctgatcg atataggcgg gctttgcagg gctccacttc
      661 acatcggtca tccccgcgag ccgtccgccc ttgcgtgcga tcagggtgtg atgtttggcc
      721 cccagttcct tcatccgatc gcgatcatcc ttgagggtct cgggagtaac tacgatgtcg
      781 ccatgttcga ggtcgtcgaa gggcatcgtc atcaacaggt ccgagaggat gggtgcgtac
      841 tcttcgaaga attcttcagg tagctcgtcc tcgaatagct tcagttcgag gtcttcactc
      901 ttagccctac cctgcgcgat ccaactctcg accatgtccc aatcgagttt tcgcaagtca
      961 agtcgattct ctgcgaatac cgtcttctcc tcggccccca catgacgaag tgccgcatga
     1021 ccatcctctt cctcgacacc ccacagcgtc gcaagagtgc gctcgtaacg gatcatgtca
     1081 tcgcgaagca gggtcagacc ctctcgcccg ataccgcggc ggcgggcctc gggcagtacc
     1141 tctccgcctc cccagatgta ctccttgttc gactcgtagc cgggggcctc aggcttcatg
     1201 actccccatc cgaagaatcc gatcgcgcga tcaccttccg ttactgagta gaagtgacgc
     1261 agaccgaatg ggtcctcccg cttcatgcgg gtctcttcga gctcatccgg gtcgacgggg
     1321 tcatcgggac gcacgagcgc atggacggct cttcggaacg cgtgatgacg ttcccaggca
     1381 gcccggtcgg ctttctgcgg atcgaatagt tcgatctggt acgtggtctc tactttcgac
     1441 atggtcgcct cccccggtta gacccgcgat agtacaacgg cgagtttccg ggcgcaggca
     1501 ggaaaagggc tacccacgtg gaagagattc gaggggaaat atcgatcttg ggggagaaca
     1561 gacatatgcg cagatcggtc accgcgatcg ctggaatggc acttgtcacg tcggccttca
     1621 tcgtagcgca gccgggggcc caggctctgc cgcccctcat cgccaacgac aacttcgctg
     1681 acgctactgt aataacgtcg atacccttca ccgaagcgac ctctaacgcg ctggccacca
     1741 tcgaaacaga tgagccgcag gcctgcggtt cgctgaccaa gaccctctgg tacagatata
     1801 cgcccacaag cgacggcatc atctcgctcg gcagtaccgg gggtatgacc acgtccgtct
     1861 tcctgggaac cacacttgct gacctacgaa ccgccgcctg ttcggagtgg ggagcatatc
     1921 tcccagtttt cgctggaacc acctatcacg tccagatcgg ggccgactcg gcaagtacat
     1981 ccttcactgt gagcgccaat gctgttagcg ggatcgcggg aagagtgacc gagacatcgg
     2041 gggcccccgt ccgcaactac tgcatccagc tgtacgacgc tgacggttct tgggatgacg
     2101 cgagttacac atcagataga tctagctatc agcatcaggt cgccgaggag tacgagggga
     2161 tgtacatctt cttcgaccca acccccggga cgaagaagat cgagtttggc tgcggcgagg
     2221 gactcttcga agatgagtgg t
//
LOCUS       DATDOZ010000258         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2565423, whole genome shotgun sequence.
ACCESSION   DATDOZ010000258 DATDOZ010000000
VERSION     DATDOZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565423"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VI541_05220"
     CDS             complement(<1..1124)
                     /locus_tag="VI541_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLF70338.1"
                     /translation="MDEEKSGVTEEEAPYQGWGSEPGEAPEEIGPGPDAPGSGEFPTT
                     PFTWNLPAPVKPKTQRRGGRFFAAMLAAAIVGAVSGGAVAYKIASDRGTGTTFAPALS
                     GKAVSKPADGTIASIVENIRPSIVAVRSETVQRNLFFEAVPAEGAGTGIILDTSGRIL
                     TNAHVVENAQKIEVLLSDGRKVVATVLGRDPSSDIAVIKIDAPDLKPAPIGNSDDLHV
                     GDGVIAVGHALALPGGPTVTEGIVSALDRSIEEPNGVLLEHLIQTDAAINPGNSGGAL
                     LDIAGNVIGMNTAIASSAQNIGFAIAITPARSVVDQLIRSGSVTRPLLGVNMVKVTPE
                     VAKQYDLQVKEGALVVQVVKGSAAALVGIEPGDVIIEADG"
     gene            complement(1145..1669)
                     /locus_tag="VI541_05225"
     CDS             complement(1145..1669)
                     /locus_tag="VI541_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLF70339.1"
                     /translation="MAKTQPGSASDAMLARALAQKNTEGSLSTVAKDHDGHPFGSVMP
                     YVLDPAGHPILLASRLAEHTANFEADPRASLMISEAIHSRAADDPLALQRVTLLGLVS
                     KIDGGDVKERYIARHPYARMYVDFDDFSFYRLTVQDVRYVGGFGRMSWVTADDFSSAD
                     VPPELLEPATTAAS"
     gene            complement(1766..>2003)
                     /locus_tag="VI541_05230"
     CDS             complement(1766..>2003)
                     /locus_tag="VI541_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLF70340.1"
                     /translation="VVERVRKVVGTIDLLHCNDSRDKAGTGADRHANIGSGSIDRDML
                     LEMVRSSGAPVVILETPWPGIVEDLAWLRENLSD"
BASE COUNT          362 a          634 c          617 g          390 t
ORIGIN      
        1 ttcccatcag cctcgatgat cacgtctccg ggctctatcc ccacgagggc agccgcgctg
       61 cctttgacga cctgcaccac cagggccccc tctttcacct ggaggtcata ctgcttggcg
      121 acttctggcg tgaccttgac catgttcacc ccaagcaggg ggcgagtgac cgagccactg
      181 cggatgagct gatcgacaac cgagcgcgcc ggtgtgatcg cgatcgcgaa cccgatgttc
      241 tgcgcggagc tcgcgatggc ggtgttcatc ccgatgacgt tacccgcgat atcgagaagg
      301 gctcccccgc tgttaccagg gttgatcgcg gcatcggtct ggatgaggtg ttcgagcagg
      361 acgccgttgg gctcctcgat cgagcggtcg agcgccgaga cgatcccttc cgtaacagtc
      421 gggccccccg gcagcgccaa ggcatgaccg accgcgatga ccccgtcgcc aacatgcaga
      481 tcatcggagt ttccgatagg ggcaggtttg agatcaggtg cgtcgatctt gatcaccgca
      541 atgtcgctgc tggggtcgcg ccccaagacg gtcgcgacga ccttgcgtcc atcgctgagc
      601 agtacctcga tcttctgcgc gttctcgacg acatgtgcgt tggttaatat gcggccgctg
      661 gtatcgagga tgatcccggt accggcaccc tcggccggta cagcttcgaa gaagaggttg
      721 cgctgcaccg tttcggagcg caccgccact atagatgggc ggatgttctc gacgatcgag
      781 gcgatggtgc cgtcagcagg ctttgacacg gccttccctg agagagccgg cgcgaaggtc
      841 gtacccgtac ccctgtcact cgcgatcttg tacgcgacgg cgccccctga cacggccccc
      901 actatcgcgg cggccagcat cgcagcgaag aagcgcccgc ctcggcgctg cgtcttcggc
      961 ttcactgggg cgggcaggtt ccaagtgaag ggcgtagtcg ggaactcgcc ggatccagga
     1021 gcgtcgggcc cgggcccgat ctcctcgggg gcctcgccgg gttcagatcc ccaaccctgg
     1081 tagggggcct cttcctcggt tacgcccgac ttctcttcgt ccaaaaggtc ctcctcggtc
     1141 ggtatcagct tgcggctgtg gtggcgggct cgagcagctc cggcgggaca tcggccgacg
     1201 aaaagtcatc agccgttacc caggacatcc gcccgaatcc gccgacatac ctcacgtcct
     1261 gcacggtcag gcgatagaac gagaagtcat cgaagtcgac atacatcctc gcgtaggggt
     1321 gacgtgcgat atacctctcc ttcacgtccc caccatcgat cttcgagacg aggcccaaga
     1381 gagtaaccct ctgaagagca agcggatcat ctgctgccct gctgtggatc gcctcggaga
     1441 tcatcagcga tgcgcgcggg tctgcctcga agttggcggt gtgctcggcg agtcggctcg
     1501 ctagcaggat gggatgacct gcggggtcga gcacgtaggg catcacagaa ccgaatgggt
     1561 gcccatcgtg atccttagct acggtcgaga gcgaaccctc ggtgttcttc tgcgccaggg
     1621 cacgtgccag catggcatcg cttgccgagc cgggctgagt cttagccatc atgtccctcc
     1681 atcgtgatag gcgaacgtca aagatccgtg accgacagat taaggcctta tgaagatagt
     1741 ggctgcgggc cgcgaggatg acggctcagt cggagagatt ctctctaagc caggctaggt
     1801 cttcgactat ccccggccac ggtgtttcga ggatgaccac gggggccccc gaggagcgga
     1861 ccatctccag cagcatgtct cgatcgatac ttccactgcc gatgttcgcg tgacgatccg
     1921 caccggtgcc tgccttgtcg cgcgagtcgt tgcagtggag aagatcgata gtgccaacca
     1981 ccttgcgtac cctttcaact acg
//
LOCUS       DATDOZ010000259         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3601520, whole genome shotgun sequence.
ACCESSION   DATDOZ010000259 DATDOZ010000000
VERSION     DATDOZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601520"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VI541_05235"
     CDS             complement(<1..498)
                     /locus_tag="VI541_05235"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLF70341.1"
                     /translation="MLLAVDVGNTQTHIGVFSGEKIAAEWRTSTDPRRTADELALIFQ
                     EFLSFQDLSFSRQVTGVVIASVVPALTRSLREMVERYFHFAPIVVEPGIRTGLPVMTD
                     NPREVGADRIVNSVAAFALLGGPVIAIDFGTATTFDVVSHDGHYLGGAIAPGVTISAS
                     ALWNIA"
     gene            complement(499..861)
                     /gene="panD"
                     /locus_tag="VI541_05240"
     CDS             complement(499..861)
                     /gene="panD"
                     /locus_tag="VI541_05240"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HLF70342.1"
                     /translation="MRRTMMHGKIHRATVTDANIDYVGSITLDPDLFEAAGILPFEKV
                     AVVDIDNGARFETYVIEGDRGSGDVILNGAAARLVQKGDKVIVIAYAEMDDAEAAAFV
                     PRVVLVDDSNRPVTFATH"
     gene            complement(923..1765)
                     /gene="panC"
                     /locus_tag="VI541_05245"
     CDS             complement(923..1765)
                     /gene="panC"
                     /locus_tag="VI541_05245"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLF70343.1"
                     /translation="MSVLERKAEVRALLERTRAEGKTVGFVPTMGFLHEGHLALVERA
                     VKGNDLCVVSIFVNPLQFESNEDLTAYPRDLERDLAMLEGAGVEVVFTPLEDEMYPGR
                     RLDTRITVGPLSRVGEGAFRPGHFDGVATVCTKLLSIVGPCRAYLGRKDAQQVAVLIR
                     LVRDLDLPVEIVPCPTVREQDGLAMSSRNVRLGPDERRAAAAIPKTLFEARDAAMSGT
                     RSAAELETIIERGLQTEPLIKLQYVEVVDPATFLRVEQVEGAALITLAAFVGSTRLID
                     NVQI"
     gene            complement(1762..2616)
                     /locus_tag="VI541_05250"
     CDS             complement(1762..2616)
                     /locus_tag="VI541_05250"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HLF70344.1"
                     /translation="MSTSDRSLRFALVGPGRVGLAMASLLRSGGHRVVRAVGRSAGSL
                     DRAERTLGCEVTTDLAGATDGAEAIMIAIPDDQIAHVAARLAQVSEVSDKVFFHTAGA
                     LGQAPLSPLARLGGRTAAVHVLQAIPDVAAGVARIPGSWFGVTCDDSLRAWCAELVSD
                     LGGHVLWVDESRRGAYHAAAVIASNFMVALGGLVETVGGGIAPYLPLMAGTLRNIEEF
                     GPLVALTGPAVRGDEGTIRDHLSVLRDDPPAMQAYIALTLVALDLAVKSDRISREKYA
                     SMVEVLKS"
     gene            2665..>3280
                     /locus_tag="VI541_05255"
     CDS             2665..>3280
                     /locus_tag="VI541_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70345.1"
                     /translation="MKRLLQVIFVSMIVASPWVRLPVAWACSCVASTPSEDFARADVV
                     VSGTASGREDPNANSEVQGGGDQITWSIQVDAVQKGEASRDLRVSSARDEAACGFEFE
                     VGRRYQVFAASVDGGFRTNLCSGTHQLAAGETPFTPEQSRPPENSPPPPITPSAGPTV
                     EPSTPPSGGPTSPTATSQSPPTRIEGVSTGTRIAIGAAILAVAAL"
BASE COUNT          646 a         1048 c         1004 g          582 t
ORIGIN      
        1 ggcgatattc cacagggccg aggccgagat cgtgacgccc ggagcgattg cgcctccgag
       61 gtagtggcca tcatggctca ccacatcgaa cgtggtcgca gttccgaaat cgatggcgat
      121 gacggggccc ccgaggagcg cgaaggccgc aaccgagttg acgatccggt ccgctcctac
      181 ctcgcgcgga ttatcggtca tgacgggaag tcctgttcgt atgccaggct cgaccacaat
      241 gggggcgaag tggaagtagc gttcgaccat ctcgcgaagc gatctcgtca gcgccgggac
      301 gaccgatgcg ataaccacgc cggtcacctg gcgcgagaag gagaggtctt ggaacgatag
      361 gaactcctga aagatgagag cgagctcatc cgctgtacga cgaggatccg ttgaggttcg
      421 ccactcggca gcgatcttct cgccggagaa aactcctatg tgagtctggg tgttgccgac
      481 gtcgacggca aggagcatct agtgcgttgc gaaggtcaca ggacggttgg agtcatcgac
      541 caggaccacg cgcggcacga atgctgcggc ctcggcgtcg tccatctcgg catacgcgat
      601 gacgatcacc ttgtcgccct tctggacgag tcttgcggcc gctccgttga ggatgacatc
      661 tcccgaaccg cgatcgcctt cgatgacata cgtctcgaac cttgcaccgt tgtcgatgtc
      721 gacgacggct actttctcga aaggcagtat gccagcggcc tcgaagaggt ccggatccaa
      781 agtgatgctg ccgacatagt cgatgttggc gtccgttacg gtggcacggt ggatcttccc
      841 gtgcatcatt gttcgtctca tcagtccctc cgcttcaggc cggacaagcc ggtcccgaca
      901 gacttctctt acttaccggc ctctatattt gcacgttatc gatcaaacgc gtcgacccga
      961 cgaaggcggc cagcgtgatg agggccgctc cttcgacctg ttcgaccctc agaaatgtag
     1021 ccggatccac aacctcgaca tactgaagct tgatcaaagg ctccgtctga aggcccctct
     1081 cgatgatcgt ttctaactcg gccgcgctgc gcgtgcccga catcgctgca tcgcgcgcct
     1141 cgaatagagt cttcgggatg gcagccgcgg ctcgccgttc gtcggggccg agacgaacat
     1201 tgcggctgga catcgcgagt ccgtcctgct cacgaaccgt ggggcatggc actatctcga
     1261 cgggcagatc gagatctcgg accagccgga tgagtaccgc aacctgctgc gcgtccttgc
     1321 gaccgagata ggcgcgacat ggtccaacta tcgaaaggag tttggtgcag accgtcgcga
     1381 cgccgtcgaa gtgaccgggc cgaaaggctc cctcacccac ccggctcaaa ggccccaccg
     1441 tgatgcgcgt gtcaagcctg cgccctgggt acatctcatc ttccagcggc gtgaacacga
     1501 cctcgacgcc cgcgccctca agcatggcga gatctcgctc gaggtctcgc gggtaggcgg
     1561 tcaggtcttc attcgattcg aactgcagtg gattgacgaa gatcgagacg acacagagat
     1621 cgttcccctt aaccgcgcgt tcgacgagtg ccaggtggcc ttcatgcagg aagcccatcg
     1681 tggggacgaa cccgacggtc ttgccctcgg cgcgcgtccg ttcaagtaga gccctcactt
     1741 cggccttgcg ctcgagaaca ctcatgactt caggacctcg accatcgagg cgtacttctc
     1801 gcgcgagatg cgatcgctct tcactgcaag gtcgagagcg accaacgtga gggcgatgta
     1861 cgcctgcatc gccggtgggt catcacgcag aacactcagg tgatcgcgga tggttccttc
     1921 gtccccgcgg acggccggtc ctgtcagcgc gaccaggggg ccgaactctt ctatgttgcg
     1981 aagcgttccc gccatcaagg ggaggtatgg agcgatgccg ccgcccacgg tctcgacaag
     2041 gccccccagt gcgaccatga agttcgacgc tatcactgcg gctgcatggt aggcgcccct
     2101 tcgtgactca tcgacccaca agacgtgacc tccaagatcc gaaaccaact ctgcacacca
     2161 ggccctaagc gagtcatcgc aagtgacgcc gaaccaggac ccagggatgc gagcaacgcc
     2221 agctgcgaca tccggtatcg cctgaaggac gtggaccgcg gccgttcggc ccccaagacg
     2281 ggcgagtggc gagagggggg cctgaccgag tgcaccggcg gtatggaaga agaccttgtc
     2341 cgaaacctcc gatacctgcg ccaatcgagc ggcgacgtga gcgatctggt catcgggtat
     2401 cgcgatcatg atggcctcgg ctccatcggt ggcccctgcg agatcggtcg tcacctcgca
     2461 gccgagtgtc cgctctgctc ggtccagaga tccggccgac cttcccaccg cgcggaccac
     2521 ccggtgaccc ccgctccgga ggagcgacgc catggccaga cccaccctgc ccgggccgac
     2581 cagagcgaag cgcagcgatc tatctgaagt agacatcggt gggacggatg gacttcacct
     2641 cgaaagtgtg gacaatgacc agcgatgaaa cgactactgc aggttatctt cgtatcgatg
     2701 atcgtggcga gcccctgggt gcggctcccc gtcgcgtggg cctgctcctg cgtcgcctcg
     2761 acaccgagtg aggacttcgc aagagctgat gtggtcgtct cgggaactgc gagcggcagg
     2821 gaagacccca acgcgaactc ggaagttcag ggcggtggcg accagatcac ctggtcgatc
     2881 caggtcgatg cggtccagaa gggggaagcg tcgcgcgatc ttcgcgtgag cagtgcgagg
     2941 gacgaagcgg catgcggatt cgagttcgaa gtggggaggc gttaccaggt gttcgctgcc
     3001 tcggtcgatg gagggttccg gacgaacctc tgctcaggga cccaccaact agctgctggc
     3061 gagaccccat tcacacctga gcagtcacgc ccacccgaga actcaccacc tccacccatc
     3121 accccgtcag cgggaccgac cgtcgagccc tcaacgccgc catcaggggg ccccacttcg
     3181 ccgaccgcca cgagccagtc gccacccaca cggatcgagg gagtttcgac aggaacgcgt
     3241 atcgcgatcg gtgccgccat ccttgctgta gcggccctcg
//
LOCUS       DATDOZ010000260         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_471778, whole genome shotgun sequence.
ACCESSION   DATDOZ010000260 DATDOZ010000000
VERSION     DATDOZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471778"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1806
                     /locus_tag="VI541_05260"
     CDS             133..1806
                     /locus_tag="VI541_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLF70346.1"
                     /translation="MRRASMSLAIPLFALVLPGSLRGTLAYWLLIATAAVALNLVCGL
                     AGQISLGHAALVGIGAYTAALVSRDGGPGFPWTLLAAGGAGVLAGLVFGVPSLRVRGQ
                     YLALVTLAGGFIFFRVAAGASFTGGEAGFGGITPLRLGGSDVTATGQVLAAGLVLAAA
                     VWGADVIGRSWYGRELAATKDSEIGAESIGVDVMRTKMTIFVLSSVIAAVAGAMLAHT
                     EGFISAQAFDVELSLSLVIACIIGGLGRPLGAVVGVFIIALPSLWGPQVAGYGFLASA
                     VVALIVLFAFPEGIVGTLRLRRGLYGLGSLRPVAGKHQVPPHSVTAAGIEIHLLSKRF
                     GNVVALDGVSITCAPGRIHAVIGPNGSGKTTLVNCMTGVLIPDSGKVDIDGVDVTGWR
                     PSRVARLGMGRTFQEQVAFPSLTVLESAALGAGRRDPTPRAMQALALVGLTVDPTASS
                     ADLSYGQARRLELARVLASTPRILVLDEPSAGRNATESEDLVALLRELREVGMTILLV
                     DHNMDLVMKVADVVTVLEDGRVLTQGKPSDVSRHPEVVRAYLGPRVVSK"
     gene            1836..>2534
                     /locus_tag="VI541_05265"
     CDS             1836..>2534
                     /locus_tag="VI541_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF70347.1"
                     /translation="MSAGWRSGAEVLHGVSVAVAEHEIVSILGSNGAGKSTLLSVIAG
                     LLSPWAGSVHFEGELISGLPAHTLVGRGLVMVPSGRKVFARQTVTDNLLMGATPYRGD
                     RVRVRRNLESTLVRFPSLMAKRREKAAQLSGGEQQLLCLARGLMARPRLLMLDEPTMG
                     LQPSALSGIFELIRELRDEGTTVLMVEKHASAAAEISDRLLVMELGSVVLSGPSSEVI
                     SDERVVGAYLGSSAP"
BASE COUNT          379 a          777 c          840 g          538 t
ORIGIN      
        1 cgggtaccgc gatgccttcg ccttcgccgt gctcatcgtg gttctgctgg tcaagccgcg
       61 cggtctcttc ggcccccgtg cgcagcgttt cgaacagata tgaggtctcc ccggatgtca
      121 gggatcaaca tcatgcgccg cgcttcgatg tcgctggcga tcccgctgtt cgcgctcgtg
      181 cttccgggtt ctttgagggg aacgctcgcg tattggctcc tcatcgctac ggcagcggtc
      241 gcgctcaacc tcgtctgcgg tctcgctggt cagatatcgc tcggtcacgc agcgctcgtc
      301 ggcatcggcg catacacggc cgcgcttgtg agtcgcgatg ggggtcctgg gttcccctgg
      361 acgttgctcg ctgccggggg agcaggtgtc ttggcggggt tggtgttcgg ggtccccagc
      421 cttcgagtgc gcggccagta tctcgccttg gtcacactcg ctggcggctt catcttcttc
      481 cgcgttgccg cgggcgcctc cttcacggga ggcgaggcgg gcttcggcgg gatcactccc
      541 ctgcgtctgg gagggtccga tgtgaccgcc accggacagg ttctagcggc tggtctcgta
      601 cttgcggccg cggtctgggg agctgatgtc atcgggaggt cttggtacgg acgagagctt
      661 gcggcaacga aggattccga gatcggcgca gagtctatag gggtcgatgt catgagaacg
      721 aagatgacga tcttcgttct gtcctcggtg atcgctgcag ttgcgggtgc gatgcttgct
      781 cacaccgaag gtttcatctc ggctcaagct ttcgacgtcg agttgtcact gtctctcgtg
      841 atcgcctgca tcatcggcgg cctcggcaga ccactcggcg cggttgtcgg cgtctttatc
      901 atcgctctgc cctcgctatg gggcccccaa gttgcgggat acggcttcct cgcatcggcg
      961 gttgtcgctc tgatcgttct cttcgcgttc cccgaaggca tcgtcggtac tcttcgcctg
     1021 cgccgcggtc tgtatggcct gggatcgttg cgccctgtcg cagggaagca tcaggtgccg
     1081 ccgcattcgg tcacagcagc ggggatcgag atccatctgc tcagcaagcg gttcggcaac
     1141 gtcgttgctc tcgacggtgt ctccatcacc tgcgcgcccg gccgcatcca tgcagtgatc
     1201 ggcccgaacg gcagcgggaa gaccactctg gtcaactgta tgaccggcgt cctcatcccg
     1261 gattcgggga aggtcgacat cgatggagtc gatgtgaccg gatggagacc cagcagagtc
     1321 gcgcgtcttg gcatggggcg aacatttcag gagcaggttg cgttcccctc gctgacggtg
     1381 ctcgagagcg cggccctggg ggccgggcgc agggacccca cgccacgagc catgcaagcg
     1441 ctcgcgctgg ttggcctgac cgtcgaccca acggcatcat ctgccgatct ctcctatgga
     1501 caggcccgca ggctcgagct cgcccgtgtg cttgcatcga ctccgcgcat cctggtgctg
     1561 gacgaaccgt cggcaggccg caacgcgacg gagtcggagg atctcgtcgc cctgttgcgc
     1621 gaacttcgtg aagtggggat gacgatcctg ctcgtcgacc acaacatgga tcttgtgatg
     1681 aaggtcgccg atgtcgtgac ggtactcgaa gacggacggg tcctcacaca gggcaaaccc
     1741 tcggatgtga gtcgccatcc cgaggtggtg cgcgcttacc tcggcccccg ggtggtctcc
     1801 aagtgacgcg cttcgaacta ctgaaggtcg agtcggtgag cgctggttgg cgatcggggg
     1861 ccgaggttct ccacggcgta tccgtcgccg tagccgagca tgagatcgtc agcatccttg
     1921 ggtccaacgg agcgggcaag tcgaccctgc tctcggtgat cgctggtctg ctctcgccat
     1981 gggcggggag cgtccatttc gagggagaac tcatctccgg actgcccgcc cacaccttgg
     2041 tagggcgggg actggtaatg gtcccgtcgg gcagaaaggt attcgcgagg cagaccgtaa
     2101 cggacaacct gctgatgggt gccacgccct atcgaggcga cagggtccgg gttaggcgca
     2161 atctcgagtc gaccctcgtg cgcttcccct cgctcatggc gaaacgccgt gagaaggcgg
     2221 ctcagctgtc gggaggggag cagcagctcc tctgcctcgc gcgtgggctt atggctcgtc
     2281 cccgtcttct catgctcgat gagccaacca tggggctaca gccgagcgct ctctctggca
     2341 tcttcgagtt gatccgggaa cttcgcgacg aaggcacaac ggtcttgatg gtcgagaagc
     2401 atgcgagtgc cgctgcagag atatcagatc gcttgcttgt gatggaactg ggttcggtcg
     2461 tgctgagtgg gccttcaagc gaggtcatct ccgacgagcg cgtggtcggc gcctacctcg
     2521 gttcgtcggc tccg
//
LOCUS       DATDOZ010000261         3081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2333325, whole genome shotgun sequence.
ACCESSION   DATDOZ010000261 DATDOZ010000000
VERSION     DATDOZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333325"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VI541_05270"
     CDS             complement(<1..299)
                     /locus_tag="VI541_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HLF70348.1"
                     /translation="MSPRVRFSIAAGVVVVSILSLIGWALSGSTSYYKTPTELASGSV
                     DPHARVRVAGKVVAGSVVVEGPTTSFEVSDGAGQIPVTTRDLLPDTFGAGVEVVA"
     gene            complement(313..2925)
                     /locus_tag="VI541_05275"
     CDS             complement(313..2925)
                     /locus_tag="VI541_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HLF70349.1"
                     /translation="MPKPSMKRSRTLFERSTTWIVITLILGGALVAGSYFGIRTIRER
                     VELSQARMFLLISGFGVVTLLLILVTYTYQVRKRLLQEDMGGTLMSWLRSHVYIGLLA
                     VVAAVAHATLFPLTNEISSGKVTGAILTLLVLSGILWRTVYARVPRLVPRRVGNLAFA
                     QTHDEAEATRVELEKLKAGRSKGFIKAVDDLVATPRKLEQIERGVASLEIGERGAWDQ
                     AKVLVEKLQRKGSAEVTQRRLTRFMQGWKAIHLPLAALLLGSIAFHVYDIANLDRFMQ
                     SEPQKQFASASDCARCHSEIVDEWRLSMHRDAGTSTTTVAQSKFALQLFPAFGKACVN
                     CHQPIGVKFSQKASFPLAADPGANPEASVEDQGVTCVVCHTMPHAPQEMAGASDKLPI
                     GKRSATSFGVMFGPPLRKPDQIPTSAHDVAVGFMTNTVTASQMCATCHNVKVDINGNG
                     VVDPALVEVVKKAGGAPIDSNANGKLDENELDIQNGTLQDLVLQTTYDEWQDYIFSND
                     GQGAACVDCHMPSAGSRPLIDRPPLSLNSEPRQRRKHIFVAVDYDLNRNYYRQKGMPR
                     DALEKVLEERERFISQAASVSVTVQAPNAEDKIIAKVTVKGLEGHSFPTGFAFARQFW
                     LEISARTPTGKQVCLTADPRGVPSPCTSGTIASVTDELATCENAGPGLSVAAEAFGPP
                     RGQRVKPASSQPLDHCDPWLTNFQKVLTDGDPDNDGTFNEVAHQSVLANVVKLRVRTA
                     DGQAVGPIPSGGSQSFDYAFDAAGAEGQPLQVKAVLRLRHLPPYFVRSLRDFEPVKTL
                     DPDKLLRNMTVVDVASNEPLPAVRTTPAPATFADARLHLTRGKSEKPKAVAVGLIGLL
                     ALAAIALRTSSSRY"
BASE COUNT          570 a          915 c          961 g          635 t
ORIGIN      
        1 tccgctacta cctcgacgcc agctccgaac gtatcgggga gaaggtcacg tgtggtcacc
       61 ggtatctgcc cagcgccatc ggacacctcg aacgaggtgg tcgggccctc gaccaccacc
      121 gaacccgcca cgaccttgcc cgcgactcgg acccgagcgt gcgggtccac gctccccgaa
      181 gctagttcgg tcggtgtctt gtagtacgaa gtggaccctg aaagagccca gccgatcagg
      241 ctcaggatgg atacgacgac gacgccagcc gcgatggaga agcggacccg tggggacatg
      301 acggtgatta tctcagtaac gcgatgagga ggttctcaga gcgatcgctg ctagtgcgag
      361 gagcccgatg agccctacgg ctacggcctt cggcttctca ctcttgcctc gtgtgagatg
      421 cagcctggcg tcggcgaaag tggcgggggc cggagtggta cggaccgcgg gcaagggctc
      481 gttggaggcc acatcgacca cagtcatgtt tcgcaggagc ttgtcggggt cgagtgtctt
      541 gactggctcg aaatcccgaa gagacctcac gaagtatgga ggcaggtggc gcagcctcag
      601 aaccgccttg acctgtagcg gttgaccttc ggcccccgct gcgtcgaacg cgtagtcgaa
      661 actctgtgac ccgcccgagg ggatggggcc caccgcttga ccgtcggcgg tgcggaccct
      721 caacttgacc acattggcca ggaccgactg atgagcgacc tcgttgaatg tcccatcatt
      781 gtctggatcg ccgtccgtga gtactttctg gaagttcgtg agccatgggt cgcaatggtc
      841 caggggctga ctcgatgcgg gtttgactcg ctgacctctc ggggggccga acgcttccgc
      901 ggcaacgctc aagccggggc ccgcgttctc gcatgtggcc agttcgtcgg tcaccgatgc
      961 gatcgttccc gatgtacagg gtgacggaac gccgcgagga tcagcggtca ggcatacctg
     1021 tttcccggtc ggggtgcggg ccgagatctc gagccagaac tgccttgcga acgcgaagcc
     1081 tgtcgggaag ctgtgccctt cgaggccctt caccgtgacc ttcgcgatga tcttgtcctc
     1141 ggcgttgggg gcctggaccg tcacgctgac gctcgccgct tgagagatga acctctcgcg
     1201 ttcttcgagc accttctcca aagcgtcgcg tggcatcccc ttttgccggt agtagttgcg
     1261 attgaggtca tagtcaaccg cgacgaagat gtgcttccga cgttggcgcg gttcgctgtt
     1321 caagcttagc ggcggtctgt cgatgagtgg cctgcttccc gctgagggca tgtgacagtc
     1381 gacacaggcg gcaccttgac cgtcgttgga gaagatgtag tcctgccact cgtcgtatgt
     1441 ggtctgtagg acgaggtcct gcagcgtgcc gttctgtatg tcgagttcgt tctcatccag
     1501 cttgccgttc gcattcgaat cgatcggtgc tccccctgcc ttcttcacta cctcgacgag
     1561 cgcgggatcg acgaccccgt tcccgttgat gtccaccttg acgttgtggc atgtcgcaca
     1621 catctgcgaa gccgtgaccg tgttggtcat gaatccaact gcaacgtcgt gcgccgaggt
     1681 cgggatctga tcaggcttgc gcaagggggg gccgaacatg acaccgaacg aagtcgccga
     1741 gcgtttcccg atcgggagtt tgtcggaggc accagccatc tcttggggag cgtgcggcat
     1801 ggtgtgacag acgacacatg tgaccccttg atcctcgacg gatgcctctg ggttagcacc
     1861 cggatcggcg gcgagaggga acgatgcctt ctggctgaac ttcacaccga tcggctgatg
     1921 gcaattgaca caagccttac cgaacgcagg gaatagttgg agagcgaact tcgactgcgc
     1981 aacggtcgtc gtgctggtgc cggcgtcacg gtgcatcgag agccgccact catcgactat
     2041 ctcgctgtga caacgtgcac agtccgatgc ggacgcgaac tgcttctgtg gttctgactg
     2101 catgaagcgg tccagattcg caatatcgta gacgtgaaac gcgatgctcc cgagaagtag
     2161 ggcggcgagt ggaaggtgga tggctttcca gccctgcatg aagcgggtga gccttcgttg
     2221 agtgacttcg gccgagccct ttctctgtag tttctcaacg agaactttgg cttgatccca
     2281 cgcgccccgt tcgccgatct cgagcgaagc gacccctcgc tcgatctgtt ccagttttcg
     2341 tggggttgcg acaaggtcat cgacggcctt gatgaatccc ttagacctgc ccgccttcag
     2401 tttctccaac tcgactcgtg tcgcctcggc ctcatcgtgc gtctgagcga aggcgagatt
     2461 cccgactcgc cttggaacca ggcgcgggac gcgtgcatac acggttcgcc agaggatgcc
     2521 gctcaggacc agcagcgtca ggatcgctcc cgtgaccttg cccgaagaga tctcgttcgt
     2581 gagcgggaag agggtggcgt gagcaacagc tgccactact gcgagtaacc cgatatagac
     2641 atgagagcgg agccaggaca tgagggtgcc ccccatgtcc tcttgaagca gtcgctttct
     2701 aacctggtat gtgtatgtga cgagtatcaa cagaagtgtg acgaccccga agccgctgat
     2761 gagaaggaac atgcgtgctt gcgagagttc gaccctctcg cggatggttc tgatcccgaa
     2821 gtaagagcca gccaccagcg cgccgccgag gatcagtgtg atgacgatcc aggtcgtcga
     2881 acgctcgaag agagtcctgc tccgcttcat cgatggcttc ggcatctaga gtttcccgct
     2941 gccggggctg acccccgtct tgccggcgat acctatcaag atgtcggagc gtccaaagac
     3001 ctcgaccggg tcgatccgca ttgcggctcc aaccggacag ttcctgacac acgcgtagtc
     3061 gtcgtatccg gcgcagaggt c
//
LOCUS       DATDOZ010000262         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2843561, whole genome shotgun sequence.
ACCESSION   DATDOZ010000262 DATDOZ010000000
VERSION     DATDOZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843561"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /gene="argH"
                     /locus_tag="VI541_05280"
     CDS             complement(<1..941)
                     /gene="argH"
                     /locus_tag="VI541_05280"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLF70350.1"
                     /translation="MSPLWSGRFSGEPAQELRRLADSIAFDVRLWPYDIAVTRAHAAG
                     LVEAGLLTADELLLIEGACDELAGLFENGEVEIHPDDEDIHSVVERFLTTRLGDTGAR
                     IHAGRSRNDLVATDMRLWAKEAVVEVARGVHGVQEALYRLARENDSAIMPGYTHLQRA
                     QPISVAHHFLAHAFAVARDFERLISAYRRADVCPLGAAAFSGTSLPVNPKATALRLGF
                     RRTFDNAADAVSSRDFLVEFLSALAMVAVNLSRIGEEIVLWTSSEFGFAALDDTYSTG
                     SSIMPQKRNPDPAELVRAKSARVTADLVTLLGVLKGLP"
     gene            complement(961..2154)
                     /locus_tag="VI541_05285"
     CDS             complement(961..2154)
                     /locus_tag="VI541_05285"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HLF70351.1"
                     /translation="MAERVVLAYSGGLDTTVAVGWLEEKKGYEVVAAAIDVGQGGDME
                     TIRERALKSGAVDAFVVDARDEFALRYAAPALHANALYQGKYPLVSALSRPLICEHLV
                     QIARSSGATAVAHGCTGKGNDQVRFEVSLGALAPELRVEAPIRDWGMTRDDAIAFGTA
                     RGLPIPVGLANPYSVDENMWGRTIECGVLEDPMVEPPDDIWERTIDATSAPREPRFIE
                     VGFEGGVPVSLDGTSLSLAQIVAQLDSAAGAYGFGRIDMIEDRVVGIKSREIYECPGS
                     LALIRAHTDLEELTLDREVLRTKRRLDMRYAELVYEGLWFTPLREAIDAFNASTSSYV
                     SGSVRLRLEPGAVRVVGRSSSNSLYDTALATYDKGDTFDHSAGAGFTKLWGLPAKVWG
                     RTHKR"
     gene            complement(2147..>2474)
                     /locus_tag="VI541_05290"
     CDS             complement(2147..>2474)
                     /locus_tag="VI541_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70352.1"
                     /translation="VYALPTEAPLLPTDALRLLLAESITEVEHGGGLLVLKTPPGHAA
                     MVASALDRSTVDGVAGTIAGDDTILIVCKPNVLPRRVERRLRSLIDGLPTPTKVASEI
                     GGRSLG"
BASE COUNT          498 a          800 c          744 g          432 t
ORIGIN      
        1 ggtaaaccct taagtacacc gagcagggtc accagatcgg cggtcacgcg ggccgatttc
       61 gcgcgtacca actctgcggg gtctgggttg cgcttctgag gcatgatgct cgaacccgtc
      121 gagtaggtat cgtccaaggc agcgaacccg aactcagatg aggtccatag aactatctct
      181 tcgccgatac gcgagaggtt caccgcgacc atcgccagcg cggagaggaa ctcgactagg
      241 aaatctcgcg atgacaccgc atccgcagca ttgtcgaaag tacgtcggaa accgagccgt
      301 agagcggtcg cctttgggtt aacgggtagg ctcgttcccg agaatgccgc cgcccccaac
      361 gggcacacat cagcccgacg ataggcggag atgagccgct cgaagtcgcg ggccaccgcg
      421 aatgcatgcg ctaggaagtg gtgagcgact gagatcggct gggccctctg cagatgcgtg
      481 taacccggca tgatcgccga gtcattctca cgcgcaagtc ggtacagggc ctcttgaacc
      541 ccgtgaaccc cccgcgcaac ttcaaccacg gcctccttgg cccacagacg catgtcggtc
      601 gcgacaagat cgttacgaga acggcccgcg tggatccggg ccccggtatc gcccagccga
      661 gtcgtcaaga aacgctcgac gactgagtgg atgtcctcat catcaggatg gatctccacc
      721 tcgccgttct cgaacagtcc ggccagctcg tcgcatgctc cttcgatgag caagagttca
      781 tcagcggtaa gtaggcccgc ttcaacaaga cccgcggcat gggcgcgggt caccgcgatg
      841 tcgtagggcc agaggcggac atcgaatgcg atcgaatccg ccaagcgacg caactcctgc
      901 gctggctccc ctgagaaccg gccgctccag agcgggctca ccagcaaccc ccttgcggac
      961 tcaacgcttg tgggtcctgc cccacacctt ggccgggagt ccccacagtt tggtgaatcc
     1021 ggcaccggcg gagtggtcga aggtgtcgcc cttgtcatag gtcgcgaggg cggtgtcgta
     1081 gagcgagttc gatgatgaac gccctaccac gcggaccgct ccaggttcta accgcaaacg
     1141 gacgcttcca gagacgtagg aagatgtcga agcattgaac gcatcgatgg cttcacgcag
     1201 cggcgtgaac cacaggccct cgtagaccaa ctccgcgtag cgcatatcga gacgacgctt
     1261 ggtgcgaagg acctcgcggt ctaacgtcaa ctcttcgaga tccgtgtgag cgcggatgag
     1321 ggcgagtgag cctggacact cgtagatctc gcgcgacttg atgcccacga cgcggtcctc
     1381 gatcatgtcg atccttccga atccgtaagc cccggccgca gagtcgagct gcgcgacgat
     1441 ctgcgctagc gaaagtgaag tgccatcgag ggacacgggg accccacctt cgaaaccgac
     1501 ttcgatgaac cgaggttccc ttggtgcgct ggtcgcgtcg atggtcctct cccagatgtc
     1561 atcgggaggt tcgaccatcg gatcctcgag gaccccgcac tcgatcgtcc gcccccacat
     1621 gttctcgtcg accgagtagg ggttcgcaag cccgaccggg atgggcaggc cgcgtgcggt
     1681 accgaaagcg atcgcatcat ctctggtcat cccccagtct ctgatgggag cttcgaccct
     1741 caactctggt gcaagagcgc caagcgagac ctcgaaacga acctgatcgt tgccctttcc
     1801 ggtgcaccca tgtgcaacgg ccgtggctcc cgatgacctc gcgatctgca cgagatgctc
     1861 gcagatgagc ggtcgtgaca gggcggacac caacgggtac ttcccctgat agagcgcgtt
     1921 cgcatgcagg gcgggggccg cgtagcgcag cgcgaactcg tcgcgggcgt cgacgacgaa
     1981 tgcatcgacc gctcctgact tgagagcgcg ctcgcgtatc gtctccatgt cgccgccctg
     2041 accaacatct atagccgcgg cgaccacttc atatcccttc ttctcctcca accacccaac
     2101 ggcgaccgta gtgtcgaggc caccggagta ggcgaggaca accctctcag ccaagtgatc
     2161 tacctccgat ctcggatgcc accttcgtcg gagtaggtaa cccatcgatg agagagcgca
     2221 gtcgcctctc gacccggcgc ggcaacacat tgggtttgca gacgatgagt atcgtgtcgt
     2281 cccccgcgat cgtgcccgcg actccatcga cggttgacct atcgagagcg gatgccacca
     2341 tcgcggcgtg ccctggcgga gtcttcagca cgagcagacc gccaccatgc tccacctcgg
     2401 tgatcgactc ggcgagcagc agccgcaggg cgtcggtcgg aagtaggggg gcttccgtcg
     2461 gaagggcata gacc
//
LOCUS       DATDOZ010000263         3328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2333771, whole genome shotgun sequence.
ACCESSION   DATDOZ010000263 DATDOZ010000000
VERSION     DATDOZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333771"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VI541_05295"
     CDS             <1..1592
                     /locus_tag="VI541_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLF70353.1"
                     /translation="RPPGVLISSWDGTSLAADIYVPTTTATSTFPLILRGHGYPGQRE
                     IRTSGSAVDDLVKAGFMVLVWDERGFGQSGGEVHLLDPAYEGRDVIALIDYVKNTEPY
                     ASKLKLDNSGNPVVGMTGGSYGGGIQWSALVADQLLGPSPTNTAATNYIDALAPEITW
                     NSLTQSLIPGGVPKIFITSLLLGTGETSSRVGGAPPPSDRFCPNTGGQDGLVETYLGG
                     AVANGQTDQSDEFLGARSIATYLSTLQVPPTFLIQGLRDTLFPPNEAIATFRAIKTQQ
                     SNSKLMLMPTGHGWSGAPPVVRSDMIDWMSNALLGSAYVNASLNTSDFIYASQGRLAS
                     SNVTTTWGTDFLYAGFDAIDSIGATGKTLAAQVAPLLTLPVASSYADVTFFQGNIDNQ
                     ADPGQAPNFDAPGTALTWDFPVGLSGADLAGTPHLTLNLTTTTPDLFLFGKFYDVDAN
                     GRASVIYHQVMPTRLRSAQGLGTTTVDWDLTALTAHIAAGHTLRLAVSTSDAAHSASR
                     FPGATLISGGSLSLPVVGGSL"
     gene            1673..2674
                     /locus_tag="VI541_05300"
     CDS             1673..2674
                     /locus_tag="VI541_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/GrpB family protein"
                     /protein_id="HLF70354.1"
                     /translation="MEPLIRFARLGDADELARLRWAFHYEDIEDVGAPGRPTIGWLDD
                     FRDLLTQALVSGRFHIWVVESDGSIVSNLWLEIVEKVPRPDDARGTWGYVTNVYTVPE
                     HRGRGIGTSMMRSVIGWAKLRRLELLVVWPSVDSMEFYGRMGFASNPRLLELNLQDIP
                     IRSSRKAVVVPHDPAWTQVFEHERAAILERLSGAVERVEHMGSTAVPGLGAKPIVDIL
                     IGVRSMGAFEDVLRGLVTLGYEYSCAAEGDMPFRRYFRKDIDGERRFQIHMVETSHDF
                     FQTHLLFRDWLRQHPDDAADYQALKEGLSQEHDEVYAYTEAKSDFILGILARARSAI"
     gene            complement(2711..>3328)
                     /locus_tag="VI541_05305"
     CDS             complement(2711..>3328)
                     /locus_tag="VI541_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70355.1"
                     /translation="DGYSLAIGIYLDEGTLRQGLECATSSGGTAIGAVHALAGKNYLV
                     QVSSGWGQEFTLTTSKTPTAPLTFEAPTPCGPLCPYWNQQTNAELDREASCAPEPASP
                     EGSWADHTVTVPSATSEWTPSALLFHIEPELDYDAWICRATPDSNGKYFVAISANLVA
                     QPCPTGVLFGCYENAVARVQPGETYRLRVYNWSDKPSTPGGYSFH"
BASE COUNT          627 a         1021 c         1017 g          663 t
ORIGIN      
        1 ccaggccccc tggcgtactg atctcctcgt gggatggcac ttcgctcgct gccgacatct
       61 atgtacccac cacaactgcc acgtcgacat tcccactcat cctgcggggt catgggtatc
      121 cgggccagcg ggagatacgt acgtcaggtt cagcagttga cgacctcgtg aaagccgggt
      181 tcatggtatt ggtctgggat gaacgggggt tcgggcagtc cggcggtgag gtgcatctgc
      241 tcgaccccgc ttacgagggc cgagacgtca tcgctctcat cgactacgtc aagaacaccg
      301 aaccctacgc gagcaaactg aagctcgaca acagcggcaa ccctgtcgtc ggtatgacgg
      361 gtggttctta cggtggtggt atccagtggt ccgcactggt ggcggatcag ctccttggcc
      421 cttcaccgac gaacacagca gctaccaact acatcgatgc ccttgcaccc gagatcacct
      481 ggaacagcct cacgcagtca ctgatccccg gtggcgttcc gaagatcttc atcactagct
      541 tgctgctcgg cacgggtgag acctcctcgc gtgtcggcgg tgcgccgcct ccctccgaca
      601 ggttctgccc aaacactgga ggacaggacg ggctagtcga gacttacctc ggtggagcag
      661 tcgccaacgg gcaaaccgat cagagcgatg aattcctcgg ggcgcgttcg atcgcgacct
      721 acctcagcac actgcaggtg ccaccgacat tcctgatcca ggggctgcgc gacacgctct
      781 tcccaccgaa cgaggccatc gcaaccttcc gcgccatcaa gactcagcag tcgaactcga
      841 agctgatgct catgcctacc ggccacggtt ggtcgggggc cccgcccgtc gttcgctccg
      901 acatgatcga ctggatgtct aacgcactct tggggtctgc gtatgtcaac gctagcctca
      961 acaccagcga cttcatctac gcgtcgcaag ggcgtctggc atcttcgaac gttacgacta
     1021 cctgggggac ggatttcctt tatgcgggtt tcgacgcgat cgactcgatc ggtgccactg
     1081 gtaagaccct tgccgcacag gtcgctccgc tgctgacgtt gcccgttgca agcagttatg
     1141 cggatgtgac cttcttccag ggcaacatcg acaatcaggc cgaccccggt caggccccca
     1201 acttcgacgc acccggaacc gccctcacct gggacttccc ggttggcctg tcgggggcag
     1261 acttggcagg tactcctcat ctgaccctga atctcacgac gacgacaccg gacctcttcc
     1321 tcttcggcaa gttctacgac gttgacgcga acggcagagc ctccgtcatc tatcaccagg
     1381 tgatgccaac gagactccgg agcgcccagg gcctcggcac gacaacggtc gattgggatc
     1441 tcaccgctct aacagcgcac atagcggcgg gacacacctt gcgtctcgca gtctcaacat
     1501 ccgatgcagc gcattcggcg agtcgcttcc caggcgccac gctgatcagc gggggctcac
     1561 tctcgctgcc ggtggtcggc gggagcctct gagcgactag aggtgctcac gaggttgccg
     1621 cacctgccac gcgaggtcat gcccctgggg tcggttatag gctgacgcga tcatggagcc
     1681 cctgattcgg ttcgcgcgtc taggtgacgc tgatgagctg gcccgtttgc gctgggcctt
     1741 tcactacgag gacatcgagg atgtcggggc ccccggtcgg ccgacgattg gttggctcga
     1801 tgacttcagg gatcttctca cgcaggcctt ggtgtcgggc cgtttccaca tctgggtcgt
     1861 ggagtccgat ggcagcatcg tctcgaacct ttggctagag atcgtcgaga aagtgccgag
     1921 accggatgat gcccgcggca cttggggtta cgtgaccaat gtctatacag tccccgaaca
     1981 ccgtggcagg ggaatcggta cttcgatgat gaggtcggtg atcggatggg caaaactcag
     2041 gaggctggag ctgctcgtcg tctggccgag tgtagattcg atggagttct atgggcgtat
     2101 ggggttcgct tcgaatcccc ggctgctcga actgaacctt caagacatcc cgatccgaag
     2161 cagcaggaag gcagtcgtgg tcccgcacga ccccgcgtgg acacaggtat ttgaacatga
     2221 gagagcagcg atcctcgagc ggttgagcgg tgcggtcgag agagtcgagc acatgggaag
     2281 caccgcggtc cccggactcg gagcgaagcc gatagtcgac atcctgatag gggtgaggtc
     2341 gatgggcgcc ttcgaggatg tcctgcgcgg gctcgtcacg ctgggctacg agtactcatg
     2401 cgcggcggag ggcgacatgc cctttcgcag atacttccgg aaggacatcg atggtgagcg
     2461 acggttccag atccacatgg ttgaaacctc ccacgacttc ttccaaacgc acctgctctt
     2521 ccgggactgg ttgagacagc atccagatga cgcagccgat tatcaggccc tcaaggaggg
     2581 gctctcgcag gaacacgacg aggtgtacgc gtacaccgag gcgaagtccg atttcatcct
     2641 cggcatcctg gcgcgtgcca gatcggccat ctaacttaag ggcctgacct caccgagatc
     2701 cgacctctgc ttagtggaag gaataccccc caggggtgct cggcttgtcg ctccagttgt
     2761 agacgcgcag tctgtatgtc tcacccggct ggactctggc aaccgcattc tcgtagcagc
     2821 cgaacaggac cccggtcgga cacggttggg caaccagatt ggctgatatg gcgacgaagt
     2881 acttcccgtt gctgtccggt gtcgcccggc agatccaagc gtcgtagtcg agttcaggct
     2941 cgatgtggaa gagcaatgcc gagggtgtcc actcactcgt tgcgcttgga accgtgacgg
     3001 tgtgatcggc ccacgaccct tccggggagg ctggctctgg cgcgcaggaa gcctcacggt
     3061 cgagttccgc gttcgtctgc tgattccagt atggacagag tgggccacag ggagtggggg
     3121 cctcgaatgt caggggagcg gttggagtct ttgaggtcgt cagtgtgaac tcctggcccc
     3181 agccactgga cacctggacg aggtagttct tgcctgcgag cgcgtgcacc gcgccgatcg
     3241 cggttccccc cgaagatgtg gcgcattcga gaccctgcct cagggttccc tcatctaggt
     3301 agatgccgat tgccagggag tagccgtc
//
LOCUS       DATDOZ010000264         3075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_220832, whole genome shotgun sequence.
ACCESSION   DATDOZ010000264 DATDOZ010000000
VERSION     DATDOZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220832"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VI541_05310"
     CDS             <1..942
                     /locus_tag="VI541_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLF70356.1"
                     /translation="KGVAGSAPGAKIMPLRVLDGNGTCSPCATSQAIHYAVDHGAKVI
                     NLSLAEVGQEVFGPGQSFTDALDYAWNRGVVTVVAAGNSYFLTSGYSNNNAIIVSATN
                     RSDGKPDFSNGVGDAKWGIAAPGGGSTFDSSSGDIYSTYWDGTTPNRYAYNAGTSMSA
                     PHVSGAAAVLRGLGLSAQQTVDRLLSTATDLGPPGRDSTFGAGRLDLAAAVKGLGSTG
                     SGGGGGGGGGTSPTPRRRSGGGSGTGGSTPVASQTPHLDPTGPAPSTSPTVAVPPEDP
                     GPPSKGVSDQGWIAAGTATALFALAGLVQWLRRRSRA"
     gene            915..1973
                     /locus_tag="VI541_05315"
     CDS             915..1973
                     /locus_tag="VI541_05315"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLF70357.1"
                     /translation="MVEKTIEGVSISHLEGETLSPRSPVAMAPIIVALLVVGLLFSTP
                     YFGTDLVALAPGPTRDAQKLMTIDATTYSSQGRFYITTALVSPGGISMAEAIKGWLDP
                     LVSIYPKDAIYPPDRSEKQATLVQARQMSDSQRDAAFAALRELGIPLDVDGLNIEQVG
                     SKIPASKSLKPGDVLLEADGMVLSRVGDLTERVQDRAVGDPIHLRFRRGNQVREADVR
                     TIASTTKGKEGKPAIGITVTLNARLPFKVEIDAQGIGGPSAGLVFAIGVYDLLTSQDL
                     TGGRVIAGTGTIDPEGQVGAVGAITQKIAGAEHRHAQVFLVPRDEYKEAKAALHSNMQ
                     LIPVSTLGEAIQALRNLR"
     gene            2044..>3075
                     /locus_tag="VI541_05320"
     CDS             2044..>3075
                     /locus_tag="VI541_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70358.1"
                     /translation="MGSLSIPFHFATTFLVTIAALGGLWVAIARPAFAPPTKWFRRLF
                     AGGWGLLALAEILHGTLIAVTDSDSTVWALRSGAYALIVFSLLPPESKTKKAGMAAVG
                     GTTAALMPTFLSGAAAFYAYRYPAPAARRLGIAFGLFAGSEFLLGFAGREAATGLTAV
                     SFVAHGLRLLGALAVGGWLRRVVRDSVQMRFVSVFVALLLMAVVVLSSVMTALFTQNI
                     TSQALSRAEREGAFQQEQLDGRRKDAVTNATTLADLDFIRNVVVKRDPPTMANTVRGL
                     QLPGKPYDRFDFISLLDPQGAILAASAGSEAAPKLTSEDQIALPGTSVVQAAITQGQP
                     VASLDVLGGG"
BASE COUNT          556 a          940 c          989 g          590 t
ORIGIN      
        1 aagggcgtcg cgggctcagc gccgggggcc aagatcatgc cgctacgtgt tctcgatggg
       61 aacggaacgt gcagtccttg tgcgacatct caggctatcc actacgccgt cgatcatggc
      121 gccaaggtga tcaacctctc actcgctgaa gttgggcagg aggtcttcgg gcccggtcag
      181 tcgttcaccg atgccctgga ctacgcgtgg aaccgcgggg tcgtcacagt ggtagcggcc
      241 gggaacagtt acttcctgac atccggctac agcaacaaca atgccatcat cgtcagcgcg
      301 accaaccgta gcgatggcaa gcccgacttc tcgaatggag tgggagacgc gaagtggggg
      361 atcgcggccc ccggaggcgg cagtaccttc gactcctcat ctggtgatat ctactccacc
      421 tattgggacg gaacgactcc gaacaggtat gcgtacaacg caggtacgag catgtccgcg
      481 cctcatgtat cgggggccgc cgcggtcctg cgggggctgg gtctcagcgc gcagcagacg
      541 gttgaccggc tgctctccac cgcgaccgac ctggggcccc cgggtcggga ttcaacattc
      601 ggtgccggcc gactggatct ggccgccgcg gtgaagggcc tgggttcgac cggatcaggc
      661 ggagggggcg gagggggcgg aggtacaagc ccgaccccaa ggcgacggtc cgggggcggt
      721 tcgggcaccg gtggctctac gccggtcgct tcgcagacgc cgcacctaga tcccacgggc
      781 cccgcgccat caaccagccc aacggtcgcc gttcctcctg aggatccagg gcccccgtcc
      841 aagggcgtct cggatcaggg gtggatcgcc gcgggcacag ccacggcact cttcgcccta
      901 gcgggactgg tgcagtggtt gagaagacga tcgagggcgt gagcatctct cacctggaag
      961 gggagacgct gtctccccgc tcacccgttg cgatggctcc catcatcgtt gcactcctag
     1021 ttgttggcct gctcttctca accccctact tcggtaccga cctcgtcgcg ctcgcaccgg
     1081 ggccgacgcg agatgctcaa aagctgatga ccatcgatgc cactacctac tcatcccagg
     1141 ggcgtttcta tataacgacg gcgctcgtct cgccgggcgg catctcgatg gcggaggcga
     1201 tcaagggctg gctcgacccc ctcgtgagca tctaccccaa ggatgcgatc tatccgccgg
     1261 accggtccga gaagcaggcg acattggttc aggctcggca gatgagcgat agtcaacgcg
     1321 atgccgcgtt cgccgcgctt cgcgagttgg ggatcccatt ggatgtggat ggactcaata
     1381 tcgagcaggt cgggagcaag atcccggcga gcaagagtct caagcccggc gacgttcttc
     1441 tcgaagcgga tgggatggtg ctgtcgcgag tgggtgacct gactgagcga gttcaagacc
     1501 gcgccgtggg cgaccccatc catctgcgtt ttcgccgagg aaaccaggtt cgtgaggcag
     1561 atgtcaggac gatcgcgtca accacgaagg gtaaggaggg caagcctgcg atcggcatca
     1621 cggtgacact caatgctcgc ctgccattca aagtcgagat cgacgctcag gggataggcg
     1681 gaccttctgc tggccttgtg ttcgctatcg gggtgtatga cctgctcact tcgcaggatc
     1741 taacaggggg ccgcgtcatc gcggggactg gcacgatcga tcccgaaggg caggtcggcg
     1801 cggtaggcgc catcacgcag aagatcgcag gtgcggaaca taggcacgcc caggtgttcc
     1861 tggttcctcg cgatgagtac aaggaggcca aggcggctct gcactcgaac atgcagctga
     1921 tacctgtttc gaccctggga gaggcgatac aggcactacg gaacctgcgt tgaaacgccg
     1981 acataacacc taaacggagt acgctaagag cgctcctccg cagaccaaat agatcgaggt
     2041 tcgatgggtt ctctgtccat tccgttccac ttcgccacta ccttcctggt cacgatcgct
     2101 gctctgggtg ggctatgggt ggcgatcgcg cggcccgcgt tcgcgcctcc cacgaagtgg
     2161 ttcagacggc tcttcgcagg aggatggggc ctgcttgctc tcgccgagat cctccatggg
     2221 accctgatag cagtcaccga ctctgactcc acggtctggg cacttcgctc gggggcttac
     2281 gcattgatcg tgttcagcct ccttccgcca gagtccaaaa ccaagaaggc ggggatggca
     2341 gcggtcggcg ggacgacagc tgcccttatg ccaacgttcc tctcaggagc ggctgccttc
     2401 tatgcctatc gctatccggc ccccgcggcg cggcggcttg ggatcgcatt cggtctcttc
     2461 gctgggtccg aattcctgct cggatttgca gggcgcgagg ctgccaccgg tctgacggca
     2521 gtctcattcg tggcgcatgg gcttaggtta ctcggggctc tcgccgtcgg aggttggctg
     2581 aggcgggttg tacgcgactc ggttcagatg agattcgttt cggtcttcgt ggctctgctt
     2641 ctcatggcgg tggtggttct gtcgagcgtg atgactgcac tgttcactca gaacatcacg
     2701 agtcaggcgc tgagtagagc cgagcgagag ggggccttcc agcaggagca gctcgatggt
     2761 cgccgcaagg atgctgtcac gaacgctaca actctggccg acctcgactt catccgaaac
     2821 gtcgttgtga agcgagaccc gccaaccatg gcgaataccg ttcgtggtct tcaactcccg
     2881 ggcaagcctt acgaccgttt cgacttcata agtttgcttg atcctcaagg tgcgatcctt
     2941 gcggcatccg cgggctcgga agcggccccc aaactgacct cggaagacca gatcgcactg
     3001 ccggggacgt cggtcgtgca ggcagcgatc acgcaggggc agcccgttgc gtctctcgat
     3061 gtactcggcg gtggg
//
LOCUS       DATDOZ010000265         4670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3918017, whole genome shotgun sequence.
ACCESSION   DATDOZ010000265 DATDOZ010000000
VERSION     DATDOZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3918017"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..952)
                     /locus_tag="VI541_05325"
     CDS             complement(47..952)
                     /locus_tag="VI541_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLF70359.1"
                     /translation="MKEIRVGVQWAPAMPGPELAAWCRELEALGYFSIGFPDHYFIGR
                     EDPFVPGEPFVSMAYAAASTTSLRLVSIVAANDFRHPALFARTIASLDLLSGGRIEAG
                     IGAGWYGPEFEAIGVPFERAGVRIERLEEALQMIKAYWTSQRFSFEGKHYRTDELPGL
                     PETVQQPHPPILIGAGGDRMLRLAGRHADVVGLTSSLRDFENRAAMMEEMKPESVSRK
                     ISIVLQAARDAERDPDSITLQTQINVIDFDGEDPGHLWALTGEPSRMIDSLQARREMG
                     ITYHMLRERNIDRIREFAEKVVSKL"
     gene            complement(976..2013)
                     /locus_tag="VI541_05330"
     CDS             complement(976..2013)
                     /locus_tag="VI541_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HLF70360.1"
                     /translation="MRLGLGVGYFGTGMTPDETLALTREADQLGYHSVWVAEAYGSDV
                     VTVLSWMGAQTKQIGLGAGIMQMTARTPAMTAMTAATLDLLSGGRVLLGLGLSGPQVV
                     EGWYGSRYDRPLQRTREYVEIVRKIIGREGPLEYDGEVFQLPLADSQGKALKLIIHPV
                     RETIPIYLASIGPRNIHLTGQIADGWLPAFYSPDHMHLFEPDLAAGAASAGRTLDDID
                     IAPTVMVSMGEDLSTCFDWLRPIYGLYLGGMGSRKDNFYGKLATRYGFEDAYLEVQEK
                     YLSGDRYAAYQAIPEELIDATALVGPPERIASRMKRYDAAGVGMLNVHTFAGSIDDRI
                     STLRTLKDLIG"
     gene            complement(2033..3229)
                     /locus_tag="VI541_05335"
     CDS             complement(2033..3229)
                     /locus_tag="VI541_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF70361.1"
                     /translation="MMDFSLTEEQKELQKWSHEFSEKEIRPVAAEYDESEEFPWPVVR
                     KAAEIGLYGFDIYLQHQQDPTGLTLPLITEELTWGCAGIALAILGTGLPLSALAAVGT
                     QEQLLTWAPRLFGTPDKPEVGAFAVTEPQAGSDVSSLSSRAVRDGDEWVLNGRKVFIT
                     NGGIADVHIVVATVDPDLGHRGQASFIVTRDTRGFSQGKKERKLGIRASHTAELILED
                     VRIPVDQVLGGIDRLDQKIATARSGERTGRSAALRTFELTRPLVAAQAVGIARAALEF
                     ATHYAKERIQFGQPIIAHQAVAHKLADMAVEVDAARLLTHRASWMGASGTPYVNAEGS
                     MSKLKASEVAVSVTEQAIQVLGGYGYIKDFPVEKFYRDAKIYTLFEGTSEMQRMVIAR
                     ALAANA"
     gene            complement(3226..4284)
                     /locus_tag="VI541_05340"
     CDS             complement(3226..4284)
                     /locus_tag="VI541_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLF70362.1"
                     /translation="MSDVSDVARRVEEATLESLADMSVDDLYTHIDVVSDQRPGPLDL
                     YRRWEAQHWSASTLDFSIDRQQWDSFDTITKTSLEEFFAGFFVGEQMVTDTLSPLVLA
                     APDEESRIFLATQLVDEARHSFFFSRFYIEVLGEPSLAAGLRRAREWTRTGSYEQIFD
                     VDLVETTDAVRLDPSNYALWVRGITLYHLLIEGVLALVGQKQLLQFLRQVDLLPAFRT
                     GFTAVTRDESRHVGYGVWAIRNAVAAGYGEDVKAAVDRSFEPCMLVYANPDYKIIVPP
                     NMTVAMVGRLIEQRWNFGLDSLTKRLRIAGLGAEYLESLAQRAGAVLVEAMDKYEANW
                     SETHPIRTLGGQTEMVAN"
     gene            complement(4350..>4670)
                     /locus_tag="VI541_05345"
     CDS             complement(4350..>4670)
                     /locus_tag="VI541_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70363.1"
                     /translation="EATAHLDSESEVLIQRALALALSGRSSLVIAHRLSTIVKADQIL
                     VVDAGRIVERGTHLELLGAGGLYGELYRTQFQTTGEIPPNGSDPDEDLMGELLTRSDL
                     RTDS"
BASE COUNT          886 a         1451 c         1407 g          926 t
ORIGIN      
        1 cggtaggcga tctccacctg ttgattcctg acggtgcccg aggttgtcaa agtttcgaca
       61 ccaccttctc ggcgaactct ctgatgcgat cgatgttgcg ctcgcggagc atgtgatagg
      121 tgatgcccat ctcgcgccgc gcttgcagag agtcgatcat gcgcgatggt tcgccggtga
      181 gggcccagag gtggccgggg tcctcgccat cgaaatcgat gacattgatc tgtgtctgca
      241 acgtgatgga gtcagggtcg cgctcggcat cccgagcggc ctggagcacg atcgatatct
      301 tccgactcac cgactctggc ttcatctcct ccatcatcgc ggcgcggttc tcgaaatctc
      361 gaagactcga cgtgagcccg actacgtcgg catgccgtcc agcgagccgc agcatgcggt
      421 cgccacccgc accgatgagg atagggggat gtggctgctg gaccgtctcc ggcagtccgg
      481 gcagttcatc ggttcgatag tgctttccct cgaatgagaa tctttgcgag gtccagtagg
      541 ccttgatcat ctggagggcc tcttccaacc tctctatccg cactccggcc cgttcgaatg
      601 gcacgccgat agcctcgaac tcggggccgt accatcccgc cccgatcccc gcctcgatgc
      661 ggcccccgga gagcaggtcc aaagaggcga tggtccgagc gaagagggct ggatgcctga
      721 agtcattcgc ggcaactatg gagacgagtc gaaggctggt cgtcgaagcc gccgcgtagg
      781 ccatgctgac gaagggttcc cctggcacga atgggtcctc gcgcccgatg aagtagtggt
      841 ctgggaatcc gatcgagaag tagccgaggg cctcaagctc tcggcaccag gctgcgagtt
      901 cgggtcccgg catggcaggg gcccattgaa cgccgacgcg tatctctttc atccgctccg
      961 tccgctccgc aagagttagc cgatgaggtc cttgagggtg cgaagggtcg agatccggtc
     1021 gtcgatgctt cccgcgaagg tgtgaacgtt gagcatccca acacccgcgg cgtcgtagcg
     1081 tttcatccgg cttgcgatgc gttccggggg cccgacgagc gcggtcgcgt cgatgagttc
     1141 ctcggggatc gcctggtatg ccgcgtatct gtcgccggaa aggtacttct cctgtacctc
     1201 gagatacgca tcttcgaatc cataccgggt cgcgagtttg ccgtagaagt tgtccttgcg
     1261 cgaccccatg cccccgagat acagaccgta gatggggcgc agccagtcga agcaggtcga
     1321 gaggtcctcg cccatcgaga ccatcaccgt cggtgcgatg tcgatgtcat cgagggtacg
     1381 acctgccgat gcggcccccg ccgcgaggtc cggttcgaat aggtgcatat ggtcgggtga
     1441 gtagaaggcc ggaagccaac cgtccgcgat ctgaccggtc aagtggatgt tgcgcggtcc
     1501 gatcgaggcc agatatatcg gaatcgtttc tcggacggga tggatgatca gcttgagggc
     1561 cttgccctgg gagtccgcga gcggcaattg aaagacctct ccgtcatatt cgagagggcc
     1621 ctcacggccg atgatcttac gaacgatctc gacatactcg cgggtccttt gtagggggcg
     1681 gtcgtatcgc gatccgtacc atccttcgac aacctggggg cccgagagtc ccaggccaag
     1741 aaggacccgg cccccggaaa gcaggtcgag ggtcgcggcg gtcatcgcag tcatagctgg
     1801 tgtgcgcgcg gtcatctgca tgatgccggc accgaggccg atctgctttg tctgggcccc
     1861 catccatgaa aggacagtga caacatcgga gccgtaggcc tcggcgaccc agaccgagtg
     1921 atagccgagc tggtcggcct cgcgggtgag ggcgagggtt tcatcgggag tcatcccggt
     1981 tccgaagtaa ccgaccccta gcccaagtct catgcgaccc cccttttcac gatcatgcgt
     2041 tggccgcaag cgctctcgcg atcaccatcc gttgcatctc ggaggttccc tcgaagagcg
     2101 tgtagatctt tgcatcccgg tagaacttct cgacggggaa gtccttgatg taaccgtaac
     2161 ccccgaggac ctggatcgcc tgttctgtga cactcaccgc gacctcgctc gccttcaact
     2221 tgctcatcga gccctcggca ttcacatacg gggtccccga tgcgcccatc caacttgctc
     2281 ggtgtgtcag cagacgcgcg gcatcgacct cgaccgccat gtctgcgagc ttatgagcga
     2341 ccgcctggtg cgcgatgatc ggctggccga actggatccg ctccttcgca tagtgggtgg
     2401 cgaactcgag agccgcacga gcgataccga cggcctgcgc cgccactagc ggacgcgtca
     2461 actcgaacgt cctcagagca gccgacctcc cggtgcgctc ccccgatcgt gcggtcgcga
     2521 tcttctgatc gagccggtcg atacctccaa gcacttgatc caccgggata cggacgtctt
     2581 cgaggatgag ctccgctgtg tgcgacgcac gtatcccgag cttgcgctcc ttcttgccct
     2641 gagagaaacc gcgcgtatcc cgcgtgacga tgaacgatgc ttgacctcta tggccgagat
     2701 cggggtctac cgtcgccacg acgatgtgca catccgcgat accgccattg gttatgaaca
     2761 ccttgcgtcc gttgaggacc cactcgtcgc catcgcgaac cgcacgacta ctaaggctcg
     2821 agacatctga gccagcctgc ggttcggtta ccgcgaacgc ccccacttcg ggtttgtcgg
     2881 gtgtgccgaa aagacgcggc gcccaggtca gcagctgctc ttgggtcccc accgcagcga
     2941 gggccgagag cggcagcccg gttcccaaga tcgcgagggc gatccccgcg caaccccagg
     3001 tgagttcttc agtgatgaga gggagggtca gcccagttgg atcctgctgg tgttgaaggt
     3061 agatatcgaa accgtagagt ccgatctcag ccgccttgcg cacaactggc caggggaact
     3121 cctcgctctc gtcatactct gccgcaactg gccggatctc cttctcggag aactcgtggg
     3181 accacttctg caactccttc tgctcttccg tgagtgagaa gtccatcagt tcgccaccat
     3241 ctcggtttgc cctcccaggg ttcttatcgg atgcgtttca gaccagttcg cttcgtactt
     3301 atccatcgcc tcgacaagca ccgctcccgc ccgctgcgcc agtgactcca gatactcggc
     3361 ccccaacccc gctatccgaa gtcgtttcgt gagtgagtcg agaccgaagt tccagcgctg
     3421 ttcgatgaga cgaccaacca tcgccacggt catgttgggc ggaacgatga tcttgtagtc
     3481 ggggttcgcg tagaccagca tgcatggctc gaatgaacgg tcgaccgcag ccttcacgtc
     3541 ttcaccgtaa cctgccgcca ccgcattgcg gatagcccaa acgccgtatc cgacgtggcg
     3601 ggactcatcg cgcgtcactg ccgtgaaccc ggtgcggaag gctgggagca gatcgacctg
     3661 ccgaaggaac tggagcagct gcttctggcc gaccagggcg agaacaccct cgatgagcag
     3721 gtggtaaagc gtgatgcccc tgacccaaag cgcgtagttg gatggatcga gcctcacagc
     3781 atcggtcgtc tccaccaggt cgacgtcgaa tatctgttca tatgaaccgg ttcgggtcca
     3841 ctcccgcgcc ctgcgaagac ctgccgcaag cgacggctca ccgaggacct cgatgtagaa
     3901 gcgtgagaag aagaaggagt gacgagcctc atccaccagt tgcgtcgcga ggaatatccg
     3961 actttcctcg tcgggtgctg ccagcactaa cggagacagt gtgtcggtga ccatctgctc
     4021 tccgacgaag aaaccggcga agaactcctc caggcttgtc ttggtgatcg tgtcgaatga
     4081 atcccactgc tgccggtcta tcgagaagtc gagagtggat gccgaccagt gctgagcctc
     4141 ccagcgacgg tagagatcca gggggcctgg gcgctgatcg ctaacaacgt cgatatgggt
     4201 gtagaggtca tcgaccgaca tgtcagccaa tgattcgagt gttgcttcct cgacgcggcg
     4261 ggcaacatca gagacatcgc tcacgttcat cctccagggt tttcggcctg cctagaactg
     4321 gactcctgaa gtctatattg acccgcaggt caggaatcgg tccgaaggtc ggaccttgtc
     4381 agcaactctc ccattaggtc ctcatcgggg tccgagccgt tgggcgggat ctctccggtc
     4441 gtctggaact gtgtccgata cagctcgccg taaagacctc ccgcgccgag caactcaagg
     4501 tgggtcccac gctcgacgat acgcccagca tcgacgacca ggatctggtc ggctttcacg
     4561 atggtcgaga gccggtgagc gatcacaaga ctggatctgc ccgacagagc cagagcaaga
     4621 gctcgttgga taaggacctc ggactccgag tcgaggtggg ctgtggcctc
//
LOCUS       DATDOZ010000266         4797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3918285, whole genome shotgun sequence.
ACCESSION   DATDOZ010000266 DATDOZ010000000
VERSION     DATDOZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3918285"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VI541_05350"
     CDS             <1..320
                     /locus_tag="VI541_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLF70364.1"
                     /translation="RRAPVIVTVVYRPSTNPRATEVEDRYAVGAAMENALLAAHSLGL
                     GAYLRTGPAALDPVVHRFLELVEGEEIAGFIYVGYPAPDEQAHLSERHPVAEVTTWRG
                     WNT"
     gene            complement(331..1746)
                     /locus_tag="VI541_05355"
     CDS             complement(331..1746)
                     /locus_tag="VI541_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLF70365.1"
                     /translation="MNEHIWAADAWQLADSVRSGALKAVELLDVCLERIGRHDSAINS
                     FVVIDPDAAHLQAQEVDRLVSDGEDPGPLAGIPIGVKDLENVEGLPTRFGSLLYDDSP
                     ALYDSTQVTRLRDAGAVILGKTTTPELGSIAYTSSKLSGITRNPWNLERTPGGSSGGS
                     AAAVAAGLVPLATASDGGGSIRIPASFTGLPGLKPTYGLVPRGPGRLGTSHLSCYGPM
                     ARSVRDIARYLDVVVGPDPMDPMSIPALDARYEEGLVMPLDGLRAVWSSDLGFGLCDP
                     EVERIARSAAEVLLSATGTREVDFPVNLPDIGMAWAVAETLDCYADLDLFWPNRSEEM
                     TPVIKLAMQIAESLQPSHISEAAKGRHEVLRLVNEIFEEVDFILTPATPTVAFDADGP
                     MPTQIGGRFVNNPLIAICFTYPFNLTGHPAMSIPAGFNSEGLPVGLQMIGPRLSESRL
                     LAAAKVMESARPWPRLARDYS"
     gene            complement(1799..2134)
                     /locus_tag="VI541_05360"
     CDS             complement(1799..2134)
                     /locus_tag="VI541_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70366.1"
                     /translation="MAIAAALKIFKDVAKVSRLPDWPIVHAEASTIDELERENLTPNY
                     PELLGVKELAELLCVSKQRVWELRNIEGFPKPLTTLASTPVWTESSVQHFLQEWKRRP
                     GRPRKAAAG"
     gene            complement(2305..2532)
                     /locus_tag="VI541_05365"
     CDS             complement(2305..2532)
                     /locus_tag="VI541_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70367.1"
                     /translation="MDKETRTLIKEALAQGWRVEELKAGYLLKAPPPSDEMVVIHGTP
                     SDTRAYANTLARMRRAGLLWPPSNRGRSKEG"
     gene            complement(2671..3537)
                     /locus_tag="VI541_05370"
     CDS             complement(2671..3537)
                     /locus_tag="VI541_05370"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLF70368.1"
                     /translation="MAPRRIGFVGLGTMGAPMAANLLGAGYTVTVHNRTRSAEEPLEA
                     AGATRASSPGEAASGADIVITMVGDTPDVEEVVFGATGIVRSASPGTLIIDMSTTDPS
                     AAASFHERVARVGLRMIDAPVSGGSEGARLGTLSIMVGGRDQDVADAMPVLEVLGGRI
                     TVVGPPGAGQMTKAINQVVIAASYTGLAEGIALGSAAGLNMEAVIEALGGGAAASWIL
                     SNRSERMLRRDYPLGFKLRLHRKDLRIALDAAKGLGITLPAAEMVARLEDQLIAQGHG
                     DEDMSVIAEAYF"
     gene            complement(3541..4407)
                     /locus_tag="VI541_05375"
     CDS             complement(3541..4407)
                     /locus_tag="VI541_05375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HLF70369.1"
                     /translation="MTTVEELLDRAEEMIGASRNVDLWRASDARVNAEELLGDVIGHE
                     ITSDDLEKDVPRSHENAFFKKVDRRVAGEPVAMILGYTEFKGMKLIVEPGVFVPRNSS
                     EFLADKAVSRIKRRKSPVAVDVATGTGPVALAIAKGVPSARVWGLDIWQPSLDVARRN
                     ARNLKLSNVTFTASDMLAGLPKQLKGTIDVFTIHPPYVARRYVRTLPKEIKGFEPAVS
                     LSDNSDDGLGLVRLLTAGAPAWLRKDGWLIVEVSSDLSRRVRGLMIRNGFTDVRSEKD
                     SLGATRVISGKI"
     gene            complement(4420..>4797)
                     /locus_tag="VI541_05380"
     CDS             complement(4420..>4797)
                     /locus_tag="VI541_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLF70370.1"
                     /translation="YDAVKDEAFTDADKRYPSTKTDLADCLIGTGFSYSRAMRERQAE
                     VVSRVLPAVRDIRRAGSCALDLAWVAAGRLDAFYEDDTHAWDISAGLTLVEAAGGAVE
                     THGALTMAAGNIELLHKLRELVL"
BASE COUNT          894 a         1515 c         1459 g          929 t
ORIGIN      
        1 cacgacgggc ccccgtgatc gtgacagtcg tgtaccgccc ctcgaccaac ccgcgcgcga
       61 ccgaggtcga agaccgttat gccgtaggtg ctgcgatgga gaacgcgctg ttagcggctc
      121 acagcctcgg gttgggggca tatctgagga cgggtcctgc cgccctcgat cctgtggtcc
      181 accgcttcct cgagttggtc gaaggcgaag agatcgcggg gttcatctac gtcggatatc
      241 cggcccccga cgagcaagcc cacctctctg agagacaccc cgtcgcagag gtgaccacct
      301 ggaggggatg gaacacctga gtgggctagg tcaggagtag tcgcgtgcga gacgtggcca
      361 ggggcgggcc gactccatga ccttggccgc ggcgaggagt cgtgactccg acaatcgcgg
      421 gccgatcatc tgtagcccga caggcagccc ctccgaattg aaacctgcgg ggatggacat
      481 cgccgggtgc cccgtaaggt tgaacggata ggtgaagcag atcgcgatca gaggattgtt
      541 aacgaacctg cctccgatct gtgttggcat gggcccgtct gcatcgaatg caacggtcgg
      601 tgttgccggg gtgaggatga agtcgacctc ttcgaatatc tcgttcacga gtctcaacac
      661 ctcatgacga cccttggctg cctcactgat atgtgacggc tgcagactct cggctatctg
      721 catcgccagc ttgatgaccg gagtcatctc ttcagatctg ttgggccaga agaggtcgag
      781 gtctgcataa cagtcgagcg tctcagctac ggcccacgcc attccgatat ccggcaggtt
      841 cactgggaag tcgacctcgc gcgtgccggt ggctgagagg agaacttcgg cggcgcttcg
      901 cgcgatcctc tcaacctctg ggtcgcagag accgaacccg aggtcggacg accacaccgc
      961 gcgcagccca tctaggggca tgacaagacc ctcttcgtaa cgggcatcga gggcagggat
     1021 ggacatcgga tccatgggat cagggcccac gactacatcg agataacgtg cgatgtcgcg
     1081 gaccgaacgg gccatcggcc cgtagcaact gagatgactc gttccgagcc tgcccggccc
     1141 ccgcggtacc aagccgtagg tcggcttgag acctggcagg ccagtgaaag atgcagggat
     1201 ccggatggac ccaccgccgt cagatgctgt cgcgaggggg acgagtcccg ccgcgaccgc
     1261 ggcagcagat ccgcccgagg atccgccggg ggtgcgttcg aggttccatg ggttgcgggt
     1321 gatgcccgag agcttcgagg aggtgtaagc gatgctgccg agttccggag tcgtcgtctt
     1381 gccaaggatc actgcccccg cgtcgcgaag tcgcgtgacc tgcgttgagt cgtagagcgc
     1441 ggggctgtcg tcgtagagca gcgagccgaa gcgagtagga agtccctcga cgttctcgag
     1501 atccttcact cctatgggga tgcccgcgag ggggcctggg tcctcaccat cgctgacgag
     1561 gcggtcgacc tcctgagcct gcagatgcgc ggcgtccggg tcgatgacca cgaatgagtt
     1621 gatcgccgaa tcatgccgac cgatccgctc gagacacaca tcgagcagtt cgaccgcttt
     1681 cagagccccg gaccgaaccg aatcagcgag ttgccacgcg tcggcggccc agatgtgttc
     1741 attcatgcag cgaggttatc tcagggggcc ttgcttcaac tagttgcagg ccgtgctgct
     1801 atccagctgc ggcctttcta ggccgccccg gccttctctt ccactcctgg agaaagtgtt
     1861 gaacgctcga ctcagtccac acgggcgtcg aagccagggt cgtcaagggc ttcggaaacc
     1921 cttcgatatt cctcagctcc caaactctct gcttcgagac gcagagcagt tccgcaagtt
     1981 ccttgactcc caatagctct gggtagtttg gcgtcagatt ctctcgttcg agttcatcga
     2041 tcgtcgacgc ctccgcatgg acgatcggcc agtccggaag tctagaaacc ttggcaacat
     2101 ccttgaagat cttcaacgca gctgcgatcg ccatggcggg atcttcggct cctacaagga
     2161 aacggactga gtaccgccgc gctcgataac ttgctacagg tgaccgatcg gcgagaatct
     2221 cgagaagaga ttcgacacta gactgctctg gcccgggtcc agagccctcg actacaatct
     2281 cgacgctcca atctggttgc tgcatcaccc ctccttggac ctacccctgt tcgacggcgg
     2341 ccacaataat cccgctcgtc gcatccgagc cagggtattg gcgtaggccc gggtatcgga
     2401 aggagtgccg tggatgacca ccatctcatc tgatggcgga ggtgctttga gcaggtaacc
     2461 cgccttcaac tcctcgactc tccacccttg cgcgagcgcc tctttgatca gagttcttgt
     2521 ttccttgtcc atcctagcaa tccttgatga tacgtcaaga gtttgtagta ctccggacag
     2581 actctaagaa gcaggtacga caaaaaaccg aggatgtcgc gcgaaccgca ggcgggtgtg
     2641 gctcagccct cggctgcccg ccgcggacgc ttagaagtag gcctcggcga tcacggacat
     2701 gtcttcgtcg ccgtggccct gtgcgatcaa ctgatcctcg agtcgggcta ccatctcggc
     2761 ggcagggaga gtgatgccga gccccttggc ggcatcgagc gcgatccgta ggtcctttcg
     2821 atgcaggcgg agtttgaaac ccaggggata gtcacgccga agcatcctct cggatcgatt
     2881 gctcaggatc cacgaggctg cggccccgcc gccgagtgct tcgatcacgg cctccatgtt
     2941 cagccccgcc gcgctgccaa gtgcgatgcc ctctgcgagg cccgtatagg acgcggcgat
     3001 cacgacctgg ttgatcgcct tggtcatctg cccggccccc gggggcccga cgacggtgat
     3061 gcgacctccg agaacttcga ggaccggcat ggcatctgcg acatcttggt cgcgaccacc
     3121 gaccattatc gagagggttc ctagacgtgc gccctcggag ccacctgaga ccggcgcatc
     3181 gatcatgcga agccccaccc tcgcgacgcg ctcgtgaaag ctcgcggccg cgctggggtc
     3241 ggtggtggac atgtcgatga tcagcgttcc aggagatgcc gatcgcacta tccccgtcgc
     3301 accgaagacc acctcctcga catcaggggt gtcgccgacc atggtgatca ctatgtccgc
     3361 cccagatgca gcctccccag gactggatgc tcgggtcgcg ccagcggctt caagcggctc
     3421 ctctgcgctt cgggtccggt tgtggacggt gactgtgtat ccggccccca gcaggttcgc
     3481 cgccatcgga gcgcccatcg tccccaaacc gacgaacccg atcctgcggg gagccaaggc
     3541 tcagatcttc ccgctgatca ctcgggtcgc gcccagagag tccttctcgc ttcgaacgtc
     3601 ggtgaagccg ttgcggatca tgagcccgcg cacgcgacgc gacaggtcgg acgacacctc
     3661 gacgatcagc cagccatcct tacgaagcca tgcgggggcc cccgccgtga ggagtctcac
     3721 gagaccgagt ccgtcgtcgg agttgtcgct gagcgagacg gcaggctcga agcccttgat
     3781 ctccttcgga agggttctaa cgtagcgtcg tgcaacgtag gggggatgga tggtgaagac
     3841 atcgatcgtg cccttgagtt gtttgggaag gccggccagc atgtcgctgg cagtgaacgt
     3901 aacgtttgaa agcttcaggt tacgggcatt tcgccttgcg acatcgaggg atggctgcca
     3961 tatgtcgagc ccccaaactc gtgccgacgg gacgcccttt gcgatcgcca gcgcgacggg
     4021 cccggttccc gttgcgacgt caaccgctac gggactcttg cgacgcttga tcctggaaac
     4081 cgccttatcg gcgaggaatt cgctcgaatt ccgaggtacg aaaactcccg gctcgacgat
     4141 cagtttcatg cccttgaact cggtgtagcc caggatcatg gcgaccggct ctcccgcaac
     4201 ccgccggtcc accttcttga agaacgcatt ctcatgcgac ctcgggacgt ccttctcaag
     4261 atcatctgac gtgatctcgt gaccgatcac atctcccagc agctcctcgg cattcacccg
     4321 cgcatcacta gcgcgccaga ggtcgacatt gcgactggcg ccgatcatct cttctgctcg
     4381 gtcgagcagt tcctcaacgg tcgtcataga gcggcgagtt tagagcacaa gttctcggag
     4441 tttgtgcagg agttcgatat ttcccgccgc catcgtcaaa gcgccatgcg tctcgacggc
     4501 tccaccggcg gcttcgacca gggttaagcc agcgctgata tcccatgcgt gggtgtcgtc
     4561 ctcgtagaac gcgtcgagcc gccctgccgc gacccaggcc agatcgagcg cgcagcttcc
     4621 cgcgcgcctg atgtcgcgaa cggcgggcag aacccgactg accacctcgg cttgtcgctc
     4681 tcgcatggcg cgcgaatatg agaagccggt tccgatgaga cagtccgcca ggtcggtctt
     4741 ggtgctgggg tacctcttat ccgcgtcggt gaatgcctcg tccttgaccg cgtcgta
//
LOCUS       DATDOZ010000267         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3606043, whole genome shotgun sequence.
ACCESSION   DATDOZ010000267 DATDOZ010000000
VERSION     DATDOZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606043"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VI541_05385"
     CDS             <1..763
                     /locus_tag="VI541_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF70371.1"
                     /translation="DHYGLVPTIRKLSGAEAFMGAHEIARLLDQRTKWELGRLLLEAG
                     FPRDLLNEMAERDRQLLKVHQVTQLECKPIQDGDIFEFDDFMLMAISLPGHTGGHLGF
                     LEPATGSMFAGDTLLPHFSPNPLLEPILEPEGEAPSSRRRSLKQYLESLDKLESLDLN
                     VVYPGHGPVITDPGRTIQYMREHHMRRLEVVWDRLDEQTLRGEAELQRILGIDRVYAR
                     VDKGHRLRHDGVRVDWSHRIGRGQRQYLDRKRSLG"
     gene            complement(821..1600)
                     /locus_tag="VI541_05390"
     CDS             complement(821..1600)
                     /locus_tag="VI541_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF70372.1"
                     /translation="MGEFVKLEVNDGVGVIRLDRPPMNALSRALVTELSEVAIEAGVS
                     EEIKAVVIWGGESIFAAGADINEFVASDTASIFHYGKYLQDTFSALAEMPKVTIAAIN
                     GFALGGGCELALTADFRFAAEDAKLGQPEILLGIIPGAGGTQRLPRLVGKSRAKELIY
                     SGKIIDAQEALRIGLVDRVLPPAEVFEKAVAIATRYARGPQVALKAAKVAIDRGGEVD
                     ITSGLAIERGAFAALFGTQDRKIGVESFFANGPGKAEFVGK"
     gene            1679..2227
                     /locus_tag="VI541_05395"
     CDS             1679..2227
                     /locus_tag="VI541_05395"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLF70373.1"
                     /translation="MIDIPSLRERLAEIEHLPAPEGVVEAAVLVPLIETETGWQLIFT
                     RRSEDLRNHGGEISFPGGRVDAHESAVEAALREAHEEVGINPREVEVLGKLRPVFTIV
                     SGYSIEPWVGVIPHNDFSPNPAEIADVIEIPIATLREPGTRRVQRFIRSGGIYRNPAF
                     DVGPNTIWGATARILDQLLEII"
     gene            2257..3135
                     /locus_tag="VI541_05400"
     CDS             2257..3135
                     /locus_tag="VI541_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70374.1"
                     /translation="MRRVFIASLTLVMLFIACTPKAPVRKGETMKQIADRTLSQLTLA
                     LPPDPELIPGTNRVVFAMLNSSGGFVPGLEVEMYIGRTQNGVAIGPVAAKFEDDGLGD
                     RSFYRADVRFPTTGKWLMLARVRGKERSPGGGATVEVKATTPVVQLGQKAVSLQTPTI
                     SDGMGLPDICSRDPEDPMHAISLDKALVSGKPTVLVIASPFFCESRVCGPVADQVLRV
                     REAFPEDANYIHVEVYTDLSDGEISKKPYTPIMTSYHLQSEPWTFVIDRDGIVRGRFE
                     GPVTTTEIKDVLLKLI"
BASE COUNT          619 a         1032 c          957 g          619 t
ORIGIN      
        1 cgatcactat ggcttggtgc cgaccatccg aaaactctcg ggggccgaag cattcatggg
       61 cgcgcacgag atcgcgaggc tgctcgacca gaggacgaag tgggagctgg gccggctcct
      121 gctcgaggcg ggcttcccgc gggatctcct caatgagatg gccgaacgcg accgccagct
      181 actcaaggtc catcaggtga cccagctgga gtgtaagccg atacaagacg gagacatctt
      241 cgagttcgat gacttcatgc tgatggcgat atcgctgccg gggcacacgg gcggccacct
      301 gggcttcctc gagccagcca ccggctcgat gttcgccgga gacaccctgt tgcctcactt
      361 cagcccgaac cctctgctcg aaccgatact cgaacccgag ggagaggccc cctcttcgcg
      421 acgccgaagc ctcaagcagt acctcgagtc gctcgacaag ctcgagtcgc tcgatctgaa
      481 tgtcgtctac ccaggccatg gtccagtgat cacggatccg ggcaggacca tccagtacat
      541 gcgcgagcat cacatgcgcc ggttggaggt tgtctgggat cgcctcgacg aacagaccct
      601 tcgcggcgaa gccgagcttc aacgcattct cggcatcgac cgtgtttacg cccgcgtcga
      661 taagggtcat cggctccgac acgatggcgt acgcgttgac tggtcccacc gcatagggcg
      721 tgggcagcga cagtacctcg accggaagcg gtccctcgga tgacatcaac ctcaggataa
      781 cggcccttac gcagtgtcgc gtcgggtctc cccacgatgg ctacttaccg acgaactcgg
      841 ccttaccggg cccgttcgcg aagaacgact cgactccgat cttgcggtcc tgcgttccga
      901 agagtgcggc gaacgcaccg cgctcgatcg cgaggcccga agtgatatcc acctctcccc
      961 ctctgtcgat cgccaccttc gctgccttga gcgcgacctg ggggcccctg gcgtagcgcg
     1021 tcgcgatagc gacggccttc tcgaaaactt cggcgggcgg aagtacgcgg tcgacgaggc
     1081 cgattcgcag ggcctcctgc gcgtcgatga tcttgcccga gtagatgagt tccttcgcgc
     1141 gcgacttccc taccagtcgc gggagtcttt gcgtgccgcc agcgccaggg atgatcccca
     1201 acaggatctc aggctgtccg agcttggcat cctcggcggc gaagcggaag tccgcggtca
     1261 atgccagctc gcacccccca ccgagcgcga agccgttgat cgctgcgatg gtgacctttg
     1321 gcatctcggc caatgcagag aaagtgtcct gaaggtactt gccatagtga aagatgctcg
     1381 ccgtgtctga agccacgaac tcgttgatat cggctccggc ggcgaatatc gactcgcctc
     1441 cccagatgac cactgccttg atctcctcgg aaacaccggc ctcgatagcc acctcgctca
     1501 actcggtgac gagggcacgg ctgagagcgt tcatcggcgg ccggtcgagc cttatcacgc
     1561 caacaccgtc gttgacttcg agtttcacga attcacccat ctcgtctctc ctttttttga
     1621 tgtctgtgtg gagtatctcg aagcactcta tccaagccag cgaacctatg attgtcggat
     1681 gatcgacatc ccttcgcttc gagagcggct cgccgagatc gaacatctcc cggcccccga
     1741 gggggttgtc gaggctgcgg tgctcgtccc tctgatcgag accgagaccg gctggcagct
     1801 catcttcacg cggcgctccg aggatcttcg caaccacgga ggtgagatct cgttccctgg
     1861 gggccgggtc gatgctcatg aatcggcagt agaggcggca cttcgtgagg cccacgagga
     1921 ggtgggcatc aacccgcgtg aggtcgaagt gctcggaaag ttgcgtccgg tcttcacgat
     1981 cgtgtcgggt tactcgatcg aaccatgggt aggcgtcatc ccccacaacg acttttcacc
     2041 gaaccctgcc gagatcgccg acgtgatcga gatccccatc gcaactctgc gagagccagg
     2101 aacccgtcgc gtccagcgat tcatccgatc tggtgggatc tatcgcaacc ccgcattcga
     2161 cgttgggccc aacaccatct ggggagccac cgcccgcatc ctcgatcaac tcctcgagat
     2221 catctagccc ctgactgcgg tagtctcctg ggcgatatgc gacgagtttt catcgcctcc
     2281 ctgacccttg tgatgctttt catcgcgtgc actcccaagg ctccggtccg caagggcgag
     2341 accatgaagc agatcgccga ccggacattg agtcagctca cgctggcctt gccgcccgac
     2401 cccgagctca tccctggaac caaccgagtg gttttcgcca tgctcaactc gtcaggtggc
     2461 ttcgttccag gactcgaagt cgagatgtac atcggccgta cgcagaacgg tgtcgcgata
     2521 ggaccagtgg cagcgaagtt cgaggacgat gggctcgggg accgctcttt ctatcgggca
     2581 gacgtgagat tcccgacgac cggcaagtgg ctcatgctcg ctcgcgtcag agggaaggag
     2641 cgatcacctg gcggtggggc aaccgtcgag gtcaaggcaa cgacgccggt ggtgcagctg
     2701 gggcagaagg cggtctcgct ccagacccca acgatctccg atgggatggg ccttcccgac
     2761 atctgcagcc gagaccccga ggacccgatg catgctatct ctctggacaa ggccctcgtc
     2821 agcgggaagc cgaccgttct ggtcatcgcg agccctttct tctgtgagag tcgagtctgt
     2881 ggtcccgtcg cagatcaagt gctcagagtg cgagaggcct ttcccgaaga tgccaactac
     2941 atccatgtcg aggtctacac cgacctcagc gacggcgaga tctcgaagaa accttacacg
     3001 cccatcatga cctcgtatca tctgcagtct gaaccctgga ccttcgttat cgacagagac
     3061 gggatcgttc gggggcgctt cgagggccca gtcaccacta cggagatcaa agacgtgctg
     3121 ctgaaactca tatgagacga aggttcacag ggctcctggg ggccgtgctg ctcgcggtgt
     3181 ggggggccct gcccgcgcag gccgggcaga ggaccgtgaa cattatg
//
LOCUS       DATDOZ010000268         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3388208, whole genome shotgun sequence.
ACCESSION   DATDOZ010000268 DATDOZ010000000
VERSION     DATDOZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388208"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1119)
                     /locus_tag="VI541_05405"
     CDS             complement(58..1119)
                     /locus_tag="VI541_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HLF70375.1"
                     /translation="MVQADIESQLVDVDRLAPFLNESIEGDGPLSVTRHIAGHSNETF
                     FVSRGEHHWVLRRPPLAVYLPTAHDVLREARVLKALNPTAVRVPRVVLVCEDVSVIGA
                     PFYLMEKVEGHVLRSDIPGVIDNPTDHRRVGEELVRSLAELHSVDPTAVGLESFGKPK
                     GYLARQVRRWTGQLELATSVTSSTRELPDMWRVRDWLTVNLPDEVLASVVHGDYKLDN
                     VIFADGSPARLRAILDWEMSTLGDPFADLGWLISLWRQKDDPPDPLHDELARITSAEG
                     FPSRAELVEMYSEFSGRRVPDLTWYVVAAIWKLACLLEGSFGRHLMGTTDDPFFARLE
                     SGVPALAKSALDIAAGGIT"
     gene            complement(1147..3657)
                     /locus_tag="VI541_05410"
     CDS             complement(1147..3657)
                     /locus_tag="VI541_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HLF70376.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLISEGEGV
                     AAKALESLGVSLDSVRSQVEEIIGSGTASPPGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLGRVRQQVIQLLQGYPAQEKGGGHASETAP
                     QGTSPILDQFGRNLTALASSNKLDPTIGREKEIERVMQVLSRRTKNNPVLIGEPGVGK
                     TAIVEGLATRIIKGEVPETLKGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRG
                     DIILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDAAL
                     ERRFQPIVVPEPTVAHTIEILKGLRDRYEAHHRIQITDQALVAAANLADRYISDRFLP
                     DKAIDLIDEAGSRLRIRRMTAPPDLREIDEKIAEVARHKVEAVESQDYEKAARLREEE
                     KVLQTEREIKDAEWQSKEGQVLAEVGEEEIAEVLSTWTGIPVYKLTEEETEKLIRMED
                     ELHKRIVDQSDAVRSVSQAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELSKTLAEFL
                     FGDEEALIQIDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRRPFSVVLFDEIE
                     KAHPDVFNTLLQILEDGRLTDSQGHTVDFKNTVIIMTSNLGTANLRKSSLGFSSDTGD
                     MTYEKMKDKVNVELKAHFRPEFLNRIDEVIVFRELSRENVREIVDKMFRRVRDQLQSQ
                     DVGIELTDAAKDVLAEEGYDPSLGARPLRRAIQRLVEDPLSEKILWKEFPAGCTILVD
                     VHEDPEHEGKRKIFFTTSGGTPAPPPPQPELAGTGADS"
     gene            complement(3825..>4377)
                     /locus_tag="VI541_05415"
     CDS             complement(3825..>4377)
                     /locus_tag="VI541_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70377.1"
                     /translation="RSQIQQGWRPQVAGFAQMTADSPARGSVAVGDYARAAWQKVPVD
                     SARGFVTSFSKRYHRRPSRDEVRGYDVVRLVDSLARGAARTDDPITALEKVDRKRFGS
                     LPISLGPLDHVLPERDHLGLWTREGGEWEPLMRTFTSDLERTDVLEEDWSAFFPGADP
                     AGEAPFFRDAKLGITTERSDDIH"
BASE COUNT          823 a         1320 c         1336 g          898 t
ORIGIN      
        1 agtcgaagac agaagttgtc aggaccccgc cgaagtcgac gatcaaaccc tggtaaatca
       61 agtgattcct cctgccgcta tatccagcgc cgacttggcg agtgccggaa cccccgactc
      121 gagtctcgcg aagaaggggt cgtcggtcgt tcccatgagg tgtcttccga aggacccctc
      181 gagaagacac gcaagcttcc agatcgcggc gacaacgtac caggtgagat cagggacgcg
      241 tcggccgctg aactcagagt acatctctac aagctcagcg cgcgacggga agccctcggc
      301 agaagttatg cgcgccaact catcgtgaag ggggtcgggc ggatcatcct tctggcgcca
      361 tagtgagatc aaccagccga ggtcagcgaa gggatcgccg agcgtggaca tctcccagtc
      421 aaggatcgca cgaagacgag ccggcgatcc atcggcgaag atcacgttgt ccagcttgta
      481 gtcaccatgg acgaccgaag caaggacctc atcggggaga ttgacggtga gccagtcacg
      541 cactctccac atgtcgggca gctcacgagt ggaactcgtc accgaagtcg ctagttccag
      601 ctgaccggtc caccggcgaa cctgtctcgc gagatagccc ttcggcttcc cgaaactttc
      661 gagcccgacc gcggtgggat cgacggagtg cagttctgcg agagatcgca ccagctcctc
      721 gccgactctg cgatggtcgg tcgggttgtc gatcacccca gggatgtcgc ttcggagcac
      781 gtgtccctcg accttctcca tcaggtagaa gggggccccg atgaccgaga catcctcgca
      841 gaccaatacg acccgcggca ccctcacggc ggttgggttc agagccttca ggacacgggc
      901 ctcgcggagc acgtcatgcg cggtgggcag gtagaccgca agcgggggcc tgcggagcac
      961 ccagtggtgt tcgcctcgcg agacgaagaa ggtctcgttg gagtggcccg ctatgtggcg
     1021 cgtgacggag agggggccgt cgccttcgat gctctcgttc agaaaggggg ccagccggtc
     1081 gacgtcgacc agttggcttt cgatgtctgc ttggaccatc tcgacatgct attcgacagg
     1141 tctgccctac gagtcggccc ccgtgccagc gagctcgggc tgtggcggag gaggagccgg
     1201 tgtgccgccg ctcgtcgtga agaagatctt ccgcttaccc tcgtgctcgg ggtcctcgtg
     1261 gacatcgacg aggatcgtgc atccggccgg gaactccttc cacaggatct tctcggagag
     1321 tggatcttcg acgagacgct ggatcgcacg acgcagcggg cgggcaccaa gtgacgggtc
     1381 atacccctct tcggcgagca cgtccttggc cgcatcggtc aactcgatgc caacatcctg
     1441 gctctgaagc tgatcgcgaa cccttcggaa catcttgtcg acgatctcgc ggacattctc
     1501 acgactgagt tcacggaaga cgatgacctc gtcgatgcgg ttgaggaact cggggcggaa
     1561 gtgcgccttc agctcgacgt tgaccttgtc tttcatcttc tcgtaggtca tatctccggt
     1621 gtccgacgag aatccgagac tcgacttgcg cagattggcc gtcccaaggt tggaggtcat
     1681 gatgatcacg gtgttcttga agtcgacagt gtggccctgg gaatcggtga ggcggccgtc
     1741 ttcgaggatc tgcagcagtg tgttgaagac gtcggggtga gccttttcga tctcatcgaa
     1801 gagcaccact gagaacggac gccgtcggac tgcctcggtg agttgcccgc cctcttcgta
     1861 tccgacatag ccgggaggcg acccgatgag tcggctcacg gtgtgcttct ccatgtactc
     1921 gctcatgtcg atctggatca aagcttcttc gtcaccgaat aggaactcgg cgagcgtctt
     1981 agaaagttca gtcttgccca caccggaggg cccgaggaag atgaaagatc cactcggacg
     2041 cttggggtcc ttgagcccgg ccctcgtgcg acggatggct tgggagactg agcggactgc
     2101 atcggactga tcgacgatcc gtttgtgaag ttcgtcctcc atgcggatca gcttctcggt
     2161 ctcttcttcg gtcaacttgt agacggggat gcctgtccat gtcgagagaa cttcggcgat
     2221 ctcctcctcg ccaacctcgg cgagaacctg accttccttg ctctgccact cagcatcttt
     2281 gatctcgcgc tcggtctgga gaaccttctc ctcttcacga agtcttgcgg ccttctcgta
     2341 atcctggctc tcgacagctt cgaccttgtg acgagccact tcagcgatct tctcgtcgat
     2401 ctcgcgcagg tctggcggag ccgtcatacg acggatgcga aggcgcgagc ccgcttcgtc
     2461 gatcaggtcg atggccttgt cgggaaggaa gcgatcggat atgtagcgat cagcgaggtt
     2521 cgctgcggcg acgagggcct gatcggtgat ctgtatgcgg tggtgagctt cgtagcggtc
     2581 acgaagaccc ttgaggattt cgatcgtgtg ggcaacggtc ggctctggga cgacgatcgg
     2641 ctggaatcgc cgctcgagag cagcatcctt ctcgaggtgc ttgcgatact cgtcgagagt
     2701 tgtcgcaccg atggtctgaa gctcgccacg agcaagcatt ggcttgagga tagaagcggc
     2761 gtcgatcgca ccttcggccg cgcccgctcc gacgagggtg tgaagctcat cgatgaacag
     2821 gatgatgtcg ccacgcgtgc ggatctcctt gaggaccttc ttgaggcgct cctcgaagtc
     2881 gccgcggtaa cgcgaaccgg cgacgagcgc accgagatcg agcgtgtaga tctgcttgcc
     2941 tttcagcgtc tcggggacct cgcccttgat gatgcgcgtg gcaaggccct cgactatcgc
     3001 cgtcttgccc acgccgggct cgccgatgag aaccggattg ttcttcgtac gtcgagatag
     3061 aacctgcatg acgcgctcga tctctttctc gcgaccgatg gtcgggtcaa gcttgttgct
     3121 cgacgcgagg gccgtgaggt tgcggccaaa ctgatcgagg atgggcgacg tgccttgtgg
     3181 ggcagtttcg gatgcatggc cgccgccctt ctcctgcgcc ggataaccct ggagcagttg
     3241 gatgacctgt tggcgaacac gtccgagatc ggcccccaac ttctgcagaa cctgagccgc
     3301 gacgccttcg ccctcgcgga tgaggccgag caggatgtgt tcggtgccga tgtagttgtg
     3361 ccctagctga agggcctcgc gaagcgagag ctctaggacc ttcttggcgc gcggcgtgaa
     3421 tgggatatgc cccggcggtg atgccgtccc cgagccgatg atctcttcaa cctgcgacct
     3481 gacggaatcg agcgagacgc ccaatgattc gagagccttt gcagcaacgc cctcgccctc
     3541 tgagatcagg ccaagcagga tgtgctcggt gccgatgtag ttgtggttga gaagtcgcgc
     3601 ctcttcctga gccaacacaa cgaccctgcg ggccctatcg gtgaagcgtt cgaacatcga
     3661 tgacatcctc gtcctccgtt actcacccat tatagggcgg tccatttgca acttaggcta
     3721 gcctaacctg ttccctgtcc atcgaacgac gccaaacttc gcataaggaa aggttcggca
     3781 cgcttggtgc attccataag ccaacttttt gaccattttt gtgactaatg gatgtcatcg
     3841 ctccgctcgg tggtgatgcc caacttcgca tcccggaaga agggggcctc tccggctgga
     3901 tccgctcccg ggaaaaatgc agaccaatcc tcctcgagga catccgtcct ctccaggtca
     3961 gacgtgaatg tacgcatcaa aggttcccac tccccgccct cccgcgtcca cagacccaga
     4021 tgatcgcgtt caggcaatac atggtcgagg gggccgagtg agataggcag tgaaccgaat
     4081 cgcttgcgat ccaccttctc cagggccgtg atcggatcgt cggttcgagc cgctccacga
     4141 gcgagcgaat cgaccaggcg cacgacgtcg tagcccctga cttcatctcg cgagggtcgg
     4201 cggtggtagc gcttgctgaa tgacgtgacg aatcctcttg ccgagtcgac ggggaccttc
     4261 tgccaagctg cccgcgcgta gtctccgaca gccaccgacc ctcgggctgg tgagtcggcc
     4321 gtcatctggg cgaaaccggc cacctgcggt ctccacccct gctggatctg cgacctt
//
LOCUS       DATDOZ010000269         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1794327, whole genome shotgun sequence.
ACCESSION   DATDOZ010000269 DATDOZ010000000
VERSION     DATDOZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794327"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /gene="ligA"
                     /locus_tag="VI541_05420"
     CDS             <1..1563
                     /gene="ligA"
                     /locus_tag="VI541_05420"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HLF70378.1"
                     /translation="VPQRLDVSHIPKVLEVRGEVYMPVGEFERVNAEMFESQGRVFSS
                     PRNAGAGSLRQKDPRITASRNLVIVCHGLGAFEGPKLTRHSEQMEYLRSVGLRVMEEA
                     GRLGSLDEVESFCRNWEGRRHENEFEADGVVIKVDALDQRQMLGYTSKSPRWAIAYKF
                     PPEEKTTRLREITVNVGRTGAVTPFAVLEPVRLSGATVSMATLHNADEIARKDIRIGD
                     IVRVRRAGEVIPEVIAPVVAKRTGKERRFKMPTQCPRCDTSLVRPEGEKVWRCPNEQC
                     PSRELESLLHFGGRGAMDIEGLGYKTVFALYERGLVADAGDIYYLTRDQILELPLFAD
                     RKADQLIAAIENSKGAGLSRVLVGLGIRHVGPPTARALASHFISIDAVAAATIEELGA
                     VDEVGEVIARAIFDFFRNPRNLEVVEKLRNAGLRLEADPSEVPSGHLVGKTFVLTGGL
                     PALSRDETTALIEGAGGKVVSSVSRKTDFVVVGENPGSKLAKAEELGVAQLDEEGLRR
                     LLAGGEGGVVES"
     gene            1628..>2043
                     /locus_tag="VI541_05425"
     CDS             1628..>2043
                     /locus_tag="VI541_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF70379.1"
                     /translation="MQLSLTDEQQAIVDALHDFAVNEVRPAARECEAEGAPIERVAKG
                     LFDMGVASPLPEEFGGQGSFDAVTSVLISEEIAWGDPGVAFGVLQSGLAATIVDALGT
                     DEQRSELLPKLADGGAFATAERDAGADITNLETTHEG"
BASE COUNT          390 a          606 c          692 g          355 t
ORIGIN      
        1 gtgccacaga gactcgatgt ctcacacatc ccgaaggtcc tcgaggtccg cggggaggtc
       61 tacatgccgg tcggggagtt cgagcgcgtc aacgccgaga tgttcgagtc gcaaggtcgc
      121 gtcttctcga gcccccgcaa cgctggagca gggtcgctgc gccagaagga tcctcggatc
      181 acggcgtcgc gtaacctcgt catcgtctgc cacggcctcg gcgcgttcga gggccctaag
      241 ctcacgcgcc actcggagca gatggagtat ctgcgctctg tcggtctgcg tgtgatggaa
      301 gaggcagggc ggctcgggag ccttgatgag gttgagagtt tctgtcgaaa ctgggagggg
      361 cgaaggcacg agaacgagtt cgaggcagac ggggtcgtga tcaaggtcga tgcgctcgac
      421 cagagacaga tgctcggcta cacgtcaaag agcccgaggt gggccatcgc atacaagttc
      481 ccaccagaag agaagacgac gcgcctacgg gagatcaccg tcaacgtggg acggacggga
      541 gcggtgaccc cgttcgccgt cctcgaaccg gtgagactct cgggggccac cgtttcgatg
      601 gcaacccttc acaacgctga cgagatcgct cgcaaggaca tccgcatcgg cgacatcgtt
      661 cgcgtgcgac gcgccggcga agtgatccca gaggtgatcg ctccagttgt cgcgaaacgc
      721 acgggcaagg agcggcgctt caagatgccc acccagtgcc cccgttgcga cacctcgttg
      781 gttcgaccag agggcgagaa ggtgtggcgc tgtccgaatg agcagtgccc atcgcgcgag
      841 ctcgagtctc tccttcactt cggtggtcgt ggcgccatgg acatagaagg gctcgggtac
      901 aagaccgtct tcgccctata cgagagggga ttggttgcgg acgcgggcga catctactat
      961 ctgacacgcg atcagatact cgagcttcct ctcttcgcgg atcgcaaggc agatcagctg
     1021 atcgccgcga tcgagaactc caagggggcg gggctctcac gtgttctggt tggcctcggc
     1081 atccgtcatg ttgggccccc gaccgcgcga gcgttggcca gccatttcat ctcgatcgac
     1141 gcggtcgctg cggccactat cgaagaactc ggcgcggtcg acgaggtggg cgaggtcatc
     1201 gctcgggcca tcttcgactt cttccgaaat cctcggaatc tcgaggtcgt cgagaagctt
     1261 cgcaacgccg ggttgcggct tgaggctgac ccctccgagg ttccgagcgg gcatctggtg
     1321 ggaaagacat tcgtcctgac cgggggcctc ccggccctca gccgtgatga gaccacggcc
     1381 cttatcgagg gggccggtgg caaggtcgtc tcttccgttt cgcgcaagac cgacttcgtt
     1441 gtggtcggcg agaaccccgg gagcaagctc gcgaaggcgg aagagctggg agtagcccaa
     1501 ctcgacgagg agggcctccg acggttgctg gcaggtgggg agggtggggt agtagaatct
     1561 tgaccctagg gtcaagaaac ctcgttgacc cgccagagct tccccaaggc caaggagggg
     1621 ttcgccagtg caactttccc taacagatga acagcaggcc atcgtcgatg ccctgcatga
     1681 tttcgcggtt aacgaggtgc gacccgcggc gcgcgagtgc gaggccgaag gcgcgcccat
     1741 cgaacgagta gcaaagggac tcttcgacat gggggtggca tcgccgctgc ccgaagagtt
     1801 cgggggccag ggcagcttcg acgcggtgac gagtgtcctc atttccgagg agatcgcctg
     1861 gggtgatcct ggcgtggcat tcggagtact ccagagcgga ctggcagcca ccatcgtcga
     1921 tgcgctcggc accgacgagc agcgctcgga gctgcttccc aagctggccg atggcggggc
     1981 cttcgcaacc gccgaacgcg atgcgggggc agacatcacg aacctcgaga cgacgcatga
     2041 agg
//
LOCUS       DATDOZ010000270         4864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1284188, whole genome shotgun sequence.
ACCESSION   DATDOZ010000270 DATDOZ010000000
VERSION     DATDOZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284188"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VI541_05430"
     CDS             complement(<1..533)
                     /locus_tag="VI541_05430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HLF70380.1"
                     /translation="MSEPSALLTVDDYEKAAKRVLPKMAYDYYRSGAEEERTLSSNRK
                     AFKRYEVWYRVLVDVSEVDTSTMVLGQRLAHPIIVAPTAYHRLAHPDGEIATAQGAEA
                     AGALMVVSTLATTRLEDVAAATRGPKWFQLYIHKDRGLTKELVQRAQESGYAALVLTV
                     DAPVLGRRIDDERNSFAL"
     gene            complement(530..1243)
                     /locus_tag="VI541_05435"
     CDS             complement(530..1243)
                     /locus_tag="VI541_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLF70381.1"
                     /translation="MSGRLLVGTSGFAYKEWKGNFYPEGTSDRKMLNYYSSRLGTVEI
                     NYTFRRLPSESTIEGWRSQTEEGFRFTLKAHQRITHYMRLREVDEQVSEFVGRAKLLG
                     DRLGTILFQLPPNMSYSREVLEHFLHCLPPSVRYAMEFRHASWDVAEVKDLLGAAGVA
                     LCGADTDEAALDEVPVLAPYVYLRLRKEDYSPDELTAWAGKIKPVLDEGVDVYCYFKH
                     EGGGVGPQLALGLLGRIGS"
     gene            complement(1248..1919)
                     /gene="nth"
                     /locus_tag="VI541_05440"
     CDS             complement(1248..1919)
                     /gene="nth"
                     /locus_tag="VI541_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLF70382.1"
                     /translation="MSASGAPNAKARDKKVLAPFPDVYRRLDRAYPGAKCALRFSNPH
                     EMLFATIMSAQCTDVMVNKVTEKLFVKYPTLEDYAGADIAELEEDIRSTGFFRQKSRN
                     IKAAANKILEEYDGTVPDTMEELITIPGAARKTANIVLGNAFGKVVGIAVDTHVRRIS
                     QRLGLTENEDPEEIEQDLMKIIPKSKWFRTSYLFIEHGRAICRAPKPRCADCVLSDLC
                     PSSTV"
     gene            1977..2585
                     /locus_tag="VI541_05445"
     CDS             1977..2585
                     /locus_tag="VI541_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLF70383.1"
                     /translation="MAIAPPISRRDQLVEVAARLFSEHGYHGTSMQQLADGLGMLRGS
                     LYAHIDSKEDLLFEIVDRGADRFLGRLDEIVRSAGPTQEKLRSAVLAHVEIVGEQIEA
                     ATVFLNEWKFLSQPRRRSVERKREQYASMMRSIIDEGIVVGTFAKDLDARFATLHMLS
                     TLNWLYQWYDPKGELTPMQVAEGFTSMLLNGFTGSYKKGRGA"
     gene            2582..3499
                     /gene="paaA"
                     /locus_tag="VI541_05450"
     CDS             2582..3499
                     /gene="paaA"
                     /locus_tag="VI541_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HLF70384.1"
                     /translation="MTDQERFAVFKAHIDAGGKVETNDWMPDEYRQAALKFVEMHANS
                     EVMGALPEREWIPRAPSLKRKMSLTAKVQDEVGHAHLLYRVAEDLGKSREAMFEDLIH
                     GRTKFHNVFHYPTEHWGDVAIIGWLIDGAALVTQRALLDCSYAPYTRVMRRICSEEQL
                     HLRHGEDITLELANGSDKQRWMFQDGLNRWWEAIMHFFGPPSVPGKDVLLRWGIKTKN
                     NEELRTEFLDHYVPRILDLGFTIPDEGLHFDEATGHWITGEIDWTMFWEIVKGNGPKT
                     QERLELRRKVWDHHRWVRDALSESIWAAA"
     gene            3654..3998
                     /locus_tag="VI541_05455"
     CDS             3654..3998
                     /locus_tag="VI541_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70385.1"
                     /translation="MEVWEVFAQKGHADPFVHVGAVAAPDEEMATAYAKECFFRRKDG
                     IAMWLVRRSDVHAPNDAQLFEQITDKSYRYPEAYRGVVEKRVAARIRGAALTGGRSAQ
                     RVEEKTAGDEYV"
     gene            3995..4759
                     /gene="paaC"
                     /locus_tag="VI541_05460"
     CDS             3995..4759
                     /gene="paaC"
                     /locus_tag="VI541_05460"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HLF70386.1"
                     /translation="MSRAIEAVLLAVADDELVMGHRHSEWTGWAPHIEEDIAFSSIAQ
                     DEIGHANLFYGLLWDLTDMSPDEIAMGRGKGEYRNSIFVERQNGDWAKTLARHLLYEA
                     AEKLRLEEFGRSSYQPLADVSEKLLREERYHQMHAEAWLHRLATGPLEGRHLFAEAIS
                     AVVPEASGFFETLEFEDEALAEGVLITPSSDLLPVWLDTVMAQIESSGLDIKERDSWI
                     ATPLTEGGRHGIHTADFDQLWDDLTRTYREEPTATW"
BASE COUNT         1002 a         1432 c         1472 g          958 t
ORIGIN      
        1 agagcgaagg agttccgttc atcgtcgatc cgtcgcccaa gaacgggggc atcgaccgtg
       61 agaaccaggg ccgcataccc cgactcctga gcacgctgga cgagttcctt ggtgagtcct
      121 cggtccttgt ggatgtacaa ctggaaccac ttcggccccc gagtcgcggc tgcgacatcc
      181 tccaacctcg ttgtggcaag ggtcgagacc accatgaggg cccctgccgc ttctgcgccc
      241 tgtgccgtcg cgatctcgcc atcggggtgg gcgaggcggt gatacgccgt gggggcaacg
      301 atgatcgggt gcgccagtcg ctgacccagg accatcgttg aggtatcgac ctcagaaacg
      361 tccacgagga cgcggtacca gacctcgtat cgcttgaacg ccttgcgatt gctgctaagc
      421 gtccgctctt cttcggcccc cgagcggtag tagtcatagg ccatcttcgg aagaacgcgt
      481 ttggccgcct tctcgtagtc atcgacagtc agcagcgccg agggctcgct caagaaccga
      541 tcctccctag aagaccaaga gccagttgcg ggccgacgcc gccaccttcg tgtttgaagt
      601 aacagtagac atcgactccc tcgtcgagga cgggtttgat cttgccagcc cacgcggtga
      661 gttcatcggg cgagtaatcc tccttacgca gacgaagata gacatatgga gcgaggacgg
      721 gcacttcatc gagggccgct tcgtcggtgt ccgctccgca tagagcgact ccagcggcac
      781 ccaatagatc cttgacctcg gctacatccc aggaggcgtg acggaactcc atcgcgtaac
      841 gaacagatgg gggcaggcag tgcaggaagt gctcgagcac ctcgcggctg tagctcatgt
      901 tcggtggaag ctgaaacagg atcgtaccga gccgatcacc gagcagtttc gcgcgcccga
      961 cgaactcgga cacctgctcg tcgacttctc tcagacgcat gtagtgagtt atgcgttggt
     1021 gagccttcag agtgaaacgg aaaccctcct cggtctgcga tcgccaacct tcgatcgtcg
     1081 actcagaagg aagtcgtctg aaggtgtagt tgatctcgac cgtgccaagg cgcgaagagt
     1141 agtagttgag catcttgcgg tcagaggtac cctccggata gaagttgccc ttccactcct
     1201 tgtaggcgaa gcccgaggtt ccgaccagta gacggccgct catgcgctca tacagtggat
     1261 gacgggcaga ggtcgcttaa gacgcagtct gcacaacgcg gtttgggggc cctgcagatc
     1321 gcccggccat gttcgatgaa caggtacgag gtccggaacc acttgctctt cgggatgatc
     1381 ttcatcaggt cctgttcgat ctcttctggg tcctcgttct ctgtgaggcc aagccgttga
     1441 gagatacgcc gtacatgtgt atcgactgcg atgccaacca ccttaccgaa agcgttgccg
     1501 aggacgatgt tggcggtctt tcgtgcggct cctgggatgg tgatgagttc ctccatcgtg
     1561 tcagggaccg tgccgtcgta ctcctcgagg atcttgttcg ccgccgcctt gatgttgcgg
     1621 gacttctgcc tgaagaatcc ggtcgagcgg atgtcctcct cgagttcagc tatgtcggct
     1681 ccggcgtagt cctccagcgt cgggtatttg acgaagagct tctcggtgac tttgttgacc
     1741 atcacatcgg tgcattgagc cgacatgatc gtggcgaaca gcatctcatg ggggttcgag
     1801 aatctcagtg cgcacttggc acccgggtag gcacgatcga gccgccgata gacatcaggg
     1861 aagggcgcga gaaccttttt gtcgcgggct ttggcgttgg gcgccccaga tgcactcata
     1921 cgtagattgt attgacccac tcaacaaaca aacgatcgtt tggtaatatt cttctgatgg
     1981 ccatcgcccc tccgatcagt cgccgcgacc agttggtcga agttgccgca aggctcttca
     2041 gcgagcatgg ctaccacggg acatcgatgc agcaactcgc cgatggactc ggcatgctca
     2101 gagggtccct ctacgcgcac atcgactcga aggaagatct tctcttcgag atcgttgatc
     2161 gcggcgccga ccggttccta ggaaggctcg atgagatcgt ccggagcgcg ggcccaactc
     2221 aggagaagct ccgatcggcg gtactcgctc atgttgagat cgtcggcgaa cagatcgaag
     2281 cggccacggt ctttctcaac gagtggaaat tcctctcgca gccgcgaaga cgctctgtag
     2341 aacgcaagcg cgaacagtac gcgtcgatga tgcgatccat catcgacgag gggatcgtcg
     2401 tgggtacttt cgccaaggat cttgatgcta ggttcgcgac actacatatg ctctcaaccc
     2461 tgaactggct atatcagtgg tacgacccaa agggcgagct gactccgatg caggtcgccg
     2521 aaggcttcac gagcatgctg ctcaatggat ttaccgggag ctacaagaaa gggagaggcg
     2581 cgtgaccgac caagagaggt ttgcggtttt caaggcccac atcgatgccg gcggcaaagt
     2641 cgagaccaac gattggatgc cagacgagta ccgccaggcg gctctgaagt tcgtagagat
     2701 gcacgccaac tccgaggtca tgggggcgct cccggaaagg gagtggatcc caagggctcc
     2761 gagcctgaaa cgcaagatgt ccctcaccgc caaggtgcag gacgaggtcg gacatgccca
     2821 tctcctctat cgagtcgctg aggacctcgg caaaagccgc gaagccatgt tcgaagacct
     2881 catccacggt cgaacgaagt ttcacaacgt cttccactac ccaaccgaac actggggtga
     2941 cgtcgcgatc atcggctggc tcatcgatgg cgcggccctc gtcacgcaac gagccctact
     3001 cgactgctcc tacgcgcctt acacacgcgt catgcgcagg atctgctctg aggaacagct
     3061 gcatcttcgc cacggcgagg acataaccct cgaacttgcg aacgggtcgg acaaacaacg
     3121 ctggatgttc caggacggcc tcaaccgatg gtgggaagcg atcatgcact tcttcggccc
     3181 tccatcggtt cccggcaagg atgttctgct ccgctggggc atcaagacca agaacaatga
     3241 agaactccgc acggaattcc tcgaccacta tgtaccccgt atcctcgacc tgggcttcac
     3301 gatccccgac gaaggtctcc acttcgatga agccaccgga cactggatca ccggtgagat
     3361 cgactggacc atgttctggg agatcgtcaa gggcaacggc cccaagaccc aggagcgcct
     3421 cgagcttcgt cgcaaagtct gggaccacca ccgctgggtc cgcgacgccc tctccgaatc
     3481 catctgggcc gccgcatgaa actaccgcca ggcccggccg agggccggtg catgctcggg
     3541 ctcccagggc ttcgccttgt cccgtcggcc gtacctcggc gcgttgggag tcttgtgggg
     3601 ccgacgagtc gccctccgca ctcaccggtc ctcggcctca tccaggtgtc gtgatggaag
     3661 tttgggaggt gtttgcgcag aaggggcatg cggatccgtt cgtgcatgtt ggggctgtgg
     3721 ctgctcctga tgaggagatg gcgacggctt atgcgaagga gtgcttcttc cggcgtaagg
     3781 atggcatcgc gatgtggttg gttagaaggt ccgatgtaca cgctcccaat gatgcgcagc
     3841 tcttcgagca gatcacggac aagtcttatc ggtatccgga ggcttaccgg ggagtggtcg
     3901 agaagagggt ggcagcccgg atcagaggtg cggccctcac cggcggcagg tctgctcaac
     3961 gggtggaaga gaagaccgcg ggagatgaat acgtatgagc cgagctatcg aggcggttct
     4021 gttggcagtc gctgatgacg agctggtgat gggtcaccga cactcagagt ggacggggtg
     4081 ggctccccac atcgaagagg acatcgcttt cagttctatc gcgcaggacg agatcggtca
     4141 cgccaacctc ttctacggac tcctttggga cttgacggac atgtcaccgg atgagatcgc
     4201 catggggcgg ggcaaaggcg agtatcgaaa ctcgatattc gtcgagagac agaacggtga
     4261 ctgggcgaaa acactcgcgc gacatctcct ctatgaggcc gccgagaagt tgcgcctcga
     4321 ggagttcggc aggtcttctt accagccctt agccgacgtc tccgagaaac tcctgaggga
     4381 agagcgatac caccagatgc acgccgaggc atggctgcac agattggcca cggggcccct
     4441 cgaaggaaga cacctcttcg ccgaggcgat ctcggcggtg gttcccgagg ctagcgggtt
     4501 cttcgagacg ctcgagttcg aagatgaggc cctcgccgag ggcgtcctca tcacaccctc
     4561 atcggatctg ctcccggtct ggctggacac ggtcatggca cagatcgagt ccagtggact
     4621 cgacatcaaa gaacgggact cttggatagc taccccgctg acggaggggg gccgtcacgg
     4681 catccacacc gcagatttcg atcaactctg ggacgacctg acccggacct accgagagga
     4741 gccaaccgcg acatggtgat ggagatgtcc ctgcgcgatc gcgccttcga cgcactcagt
     4801 caggtcaagg accccgagat ccccgcggtg tccgtcctcg atcttggcat catcgaagat
     4861 gtct
//
LOCUS       DATDOZ010000271         8729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_742494, whole genome shotgun sequence.
ACCESSION   DATDOZ010000271 DATDOZ010000000
VERSION     DATDOZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742494"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VI541_05465"
     CDS             <1..627
                     /locus_tag="VI541_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HLF70387.1"
                     /translation="WLSMDPRRTHPALRTGGPTADWTQYALDAQVMLIRLDDSRYEAI
                     TETTSFRDWIDNGHAYGFPTEDDFAYHLTTLFPPVRPRGRLEIRMIDALPDDLLRVAT
                     ATVCSLLGPQGVDEISKRSWNAGSLWYEAARYGLSHPELRRIVTESFEIALDVAEDSG
                     VDANTRSMMATYFSDHISVGRCPADDRLDRFDRAGEVLPEISREPAWS"
     gene            618..1904
                     /gene="egtB"
                     /locus_tag="VI541_05470"
     CDS             618..1904
                     /gene="egtB"
                     /locus_tag="VI541_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HLF70388.1"
                     /translation="MELKQSLANELEGVRRRSFSLLEPVADEDLMKQLSPIMSPLVWD
                     LAHVGNYEEIWLLRMVAGVPAANPELDDIYDAFKHARKERTKLRLLPPEAARGYITEI
                     RGQVFDVLEAVELDPSNALLKDGFVYGMVVQHEHQHDETMLATLQLRARQYPTKELPT
                     PAGRAIPVREVLIEGGIFQMGTDLEPWAYDNERPAHTVDLDAFWIDTYPVTNAEYMAF
                     IAAGGYDDPRHWSSEGWEWRQKEQAFAPMNWRRSGNAWSRNRFGKVEPVPPEEPVQHV
                     CWHEACAYASWVGKRLPTEAEWEVAATWDPGTSRKRRYPWGDSEPTDARANIGERRYH
                     PAPVGAYPRGINPFGCHQMIGDVWEWTSSDFLPYPGFNSFPYREYSEVFYGHDYKVLR
                     GGSWATHATVIRGTFRNWDFPIRRQIFAGFRCARDA"
     gene            1911..2663
                     /gene="egtC"
                     /locus_tag="VI541_05475"
     CDS             1911..2663
                     /gene="egtC"
                     /locus_tag="VI541_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HLF70389.1"
                     /translation="MCRMLAYSGPPVTLSRLILEPSHSLLKQSYAPRYQSVGTVNADG
                     FGVGWYDLAASSEPGVYRNDKPIWSDHSFSSIARVISSGAIVAAVRDATPPGPVEVSS
                     TAPFAAGTLLFAHNGALNGFFDGVNIEIRSMLSRNRGSQIRGSSDSEVLFAFVLDRID
                     EGASQADALRATIKACSRWGGRFNFLLHDGNGITATASGNSLFATKAAATIAEGTIIA
                     SEPLDDSAGWESIEDGYLVEASAGGFDVTELD"
     gene            2673..3644
                     /gene="egtD"
                     /locus_tag="VI541_05480"
     CDS             2673..3644
                     /gene="egtD"
                     /locus_tag="VI541_05480"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HLF70390.1"
                     /translation="MERITFDVHLGAKELREILENDARTGLTSSPKFIPSKYFYDQKG
                     SKLFDEITRLPEYYLTRAERSILERYSDEIVRRCGADTLVEIGSGTSDKTRLLLDAFW
                     NSGRLDRFIPFDVDESTLRSAALGLVSSYPDLRVHGVVGDFEENLHQLPKEGKRLIAF
                     IGSTIGNLDALGRMKFLEQLAGNMAPGDYFLVGIDLVKERATLEVAYNDSAGITAEFN
                     RNILDVLNNSLDGDFAPDRFEHVAFFDQDNSWIEMRLRSRQQQEVRIAAIDLELNLSS
                     GEEIRTEISCKFTIEQVEGELQRSGLMPLEWWLDDNGRFALGLSAKP"
     gene            3775..5598
                     /locus_tag="VI541_05485"
     CDS             3775..5598
                     /locus_tag="VI541_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF70391.1"
                     /translation="MRFIGDATAASLFLMAAAAIANYVRTRARRERTIASILALLTIT
                     SSCDRLLRHLGFDWTIAALAYGTFMLAGYQILLFRVDIFPIRRRIQPWALLVTLATTA
                     LGATFSISEEGPHTPAGVASLALSGVLWIIVLGESCLTFWRASRHRQLIQRARLRALV
                     FGYLALMVLLSVRFIDTNAPGVDLLAAPLPFFLYMGLSAPAWLRRAWRRHEEDLLPMV
                     VDLISHTDDVAEMASAGLDSALRLTGASHATLIWSDGIMASSSQPRNAGTNHTKLAEL
                     DLRMEQGTATLVLLRGPLTPELEEDGRARLTRFAKSLAIGLERAMLMDRARQEEESEA
                     EILRERARIADQLALALTIERSAHEEVEELERVRSDFIASVSHELRTPATVIKGFAAT
                     LMQSWDRLSDEERRSFVEIIRSRSDDLEELLDELLDFTRWEGGHAEIHQDLTKPKECL
                     DEVVSALGPGQAARVRNLISDDVVVVMASSALRRVLSNLVSNALKFSDESAPVLVSAV
                     DDGHKVEFAVKDQGVGVDPAEMALIFERFYRVNRTDSTASGTGIGLTLVKGFVEAGGG
                     RVWAESLPGAGSTFFFSLPSQGGSEPSHKMVPGTAGMTSKA"
     gene            5648..8710
                     /locus_tag="VI541_05490"
     CDS             5648..8710
                     /locus_tag="VI541_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70392.1"
                     /translation="MSPRHTPRGPLRFLAVAAALGLIAVIFPSAGLATLTGLRAGAAR
                     LDSTWHVGASAGQYASDGVAVGDHGVDPFTHSTRRSPSYGIQGREWVRALVVEGSDGK
                     RFAVASNDLYIPQDLLNRRVALILAEHDAKVRLGLEDGPVTRISAENLTISVSHSHSS
                     PYYSTPSWGVWAFQDVFDIRFFEHLSQKMADAVIAATASLRPARVGGAVGPFSLLKRH
                     SFGPQGANDGTPAGYPQTDIDPDLSLVRFDDVTDPQNPLPLANWVIFGLHPELLSGND
                     LLANEYVNRMETIIDREIGGVTLFSQNDTGTSEPARNRDAHTAAQRQEFSHREYGQVE
                     RAARTLASSVEALHADIATVSSNNPATYANNVVRFASDVPIRFKDLRFAPPSYRLLPT
                     VSSCRTEKAFDGNPGVPIVGLPDCAFLWNETIDEDKAPGWDQLPQDPQVTYRTLRDAG
                     VPIPDNVSAPSYTGLQETLQVHLQAIRIGDIGITVCPCEQWADQARNIKSRLNQTNGD
                     FWYGWDWTSNYTQSGWTAGTNYANTVLGWCDPSGASWICKDPRSPSTNLAPISDYTLK
                     RFKAQIYNDAKGWDALDYTTQSESEPTDISKIKGNYTHEELDGVMAGGGYPMVITVGM
                     SNDYWGYIATYREFQRGDAYRKALTGLGPHSSDFLATRLSRMAAELKGGPKVRLTAKD
                     LAYLPEYAHQGSRAEVIGQAAHNYVGAYEALLPADGGAPAIVTQPSNIKRFSAAKVSW
                     VGGSNFTDTPHAKVERCALVACDAAIDTGWVPFGNGFGEVQVKANYPQPEDMPAFTAG
                     QFEWRWDATFEAFDSDVPLPDASGTARTQTPAGQYRFVIDGCSRSPFPGAADTTCGSY
                     DPGHRVQPYHLRSGSFTVLPWDGMTIPSISSEPGGISFTVGPAYPWAITASNTERGPI
                     DYPNSYASLFKFIANRSASDVFYGGSEVFCLKCSFRPWADTGSVATAKVTVVRSGVPE
                     LVDATLGLDGRWHAAVTLAAGDEAYIAPGGIVDEFGEYNGSESAHVVG"
BASE COUNT         1774 a         2706 c         2596 g         1653 t
ORIGIN      
        1 tggctctcta tggatcctcg acgaacgcat cccgctctgc gaaccggggg cccgaccgcg
       61 gattggacgc agtacgcgct tgatgcgcag gtgatgctca tccgcctcga cgattcgaga
      121 tacgaggcca tcaccgagac gacgagcttc agggactgga tcgacaacgg gcatgcctac
      181 gggttcccga ccgaggacga cttcgcttat cacctgacga cgctgttccc cccggtcaga
      241 cccaggggcc gacttgagat ccggatgatc gatgcccttc ccgatgacct gcttcgcgtc
      301 gcgacggcaa ccgtctgctc actgctcggc ccccagggcg tggacgagat ctcgaaacgc
      361 agttggaacg cgggaagtct ctggtatgag gctgcgcgat acggtctctc tcatcctgag
      421 ttgcgacgta tcgttacaga atcattcgag atcgccctgg atgtggcgga agacagcggg
      481 gtcgatgcaa acaccagatc gatgatggcg acatacttca gcgatcacat ctctgtcggc
      541 cgatgtcctg ccgatgacag actggatagg ttcgaccggg cgggcgaggt tctgccagag
      601 atatcgaggg agcctgcatg gagctgaagc aatcccttgc gaacgagttg gagggtgttc
      661 ggcgccggtc ctttagtctg ctcgaacccg tagcagatga ggacctgatg aagcagctct
      721 ctccgatcat gtcgcccctg gtgtgggacc tagcccatgt cggcaactac gaagagatct
      781 ggttgctgcg gatggtcgct ggggtcccgg ccgcaaaccc tgaacttgac gacatctacg
      841 acgccttcaa acacgctcgc aaagaacgta cgaaactccg actactcccg cccgaggccg
      901 cgcgcggcta catcaccgaa atacgcggcc aagtgttcga tgttctcgag gcggtggagc
      961 ttgacccatc caatgcactc ctgaaggacg gcttcgtcta cgggatggtc gtgcagcatg
     1021 agcatcagca tgacgagacc atgctggcga cactgcagct gcgggcaaga cagtacccga
     1081 cgaaggagct cccgaccccc gcggggcgag cgatccccgt tcgagaggta ctgatcgagg
     1141 gtggcatatt ccagatgggg actgacctgg aaccgtgggc ctacgacaac gagcggccag
     1201 cccacaccgt cgacctggat gctttctgga tcgataccta tcccgtgacg aatgccgaat
     1261 acatggcctt catagcggct ggcggatacg acgacccacg gcactggagt tcggaggggt
     1321 gggagtggcg ccagaaggag caggccttcg ccccgatgaa ctggcgtagg tcggggaatg
     1381 catggtcgag gaaccgcttc ggcaaggtgg agcccgtccc tcctgaagaa cccgttcaac
     1441 acgtctgctg gcacgaggct tgcgcatatg cctcgtgggt aggcaagcgg cttccgaccg
     1501 aggcggagtg ggaggtggcg gccacatggg atccagggac gagccggaag cgccgctatc
     1561 cgtggggtga ctccgaaccc acagacgctc gtgccaatat cggagagcgg cgctaccatc
     1621 ccgcgccggt gggagcctac cctcgcggca tcaacccatt tggatgtcac cagatgatcg
     1681 gcgacgtctg ggagtggaca tcctcggact tccttcccta tccgggtttc aattctttcc
     1741 cataccgcga gtactctgag gtcttctacg gacatgacta caaggtcctg cgcggtggat
     1801 cctgggccac acatgcaacc gtgatccgtg ggacgttccg caactgggac tttccgatac
     1861 ggcggcagat attcgcggga ttccgctgcg ctcgagacgc ctgagagacc atgtgcagga
     1921 tgctggccta ctccggcccc ccggtgaccc tttcacgact gatactcgag ccttcgcatt
     1981 cgctgctgaa gcagtcatac gccccgcgat accagagcgt cgggactgtc aatgcggatg
     2041 ggttcggtgt cggttggtat gaccttgccg cctcgagtga acctggtgtt taccgtaacg
     2101 acaaacccat ctggagcgat cactcgttca gcagcatcgc tcgagtgatc tcttcaggtg
     2161 ccatcgtcgc cgcagtgcga gacgcaacgc cgcccggccc ggtcgaggtg agcagcaccg
     2221 cgccattcgc tgcgggcacc ctgcttttcg cgcataacgg agccctcaat ggattcttcg
     2281 acggcgtgaa catcgagatc cgcagcatgc tctcccgcaa ccgagggtcg cagatacgtg
     2341 gcagcagtga ctcggaggtt ctattcgcct ttgttctcga tcgcatcgat gagggagcat
     2401 cacaggcgga cgccctgcgc gccacgatca aagcatgttc gcgctggggg ggtcggttca
     2461 acttcctgct tcacgacggc aacgggatca cagcaacggc atcgggcaac tccctcttcg
     2521 ccacgaaggc tgccgcgacg atcgcagaag gaaccatcat cgcttctgaa ccgctagacg
     2581 actcagcggg atgggagagt atcgaggatg gatacttggt cgaggccagc gccggtggtt
     2641 tcgatgtgac cgaattggac taggctttcg cgatggagcg catcactttc gacgtgcatc
     2701 tgggggccaa ggaactcaga gagatccttg agaacgatgc ccgaaccggt ttgacctctt
     2761 caccgaaatt catcccatcc aagtacttct acgaccagaa gggctcgaag ctcttcgacg
     2821 agatcacccg cctgcccgag tactacttga cgcgggcaga gcgctccatc ctggaaaggt
     2881 actccgacga gatcgtgcgc cgctgcggag ccgacaccct tgtagagatc ggttccggaa
     2941 cctcggacaa gacacgactc ctgctcgatg ctttttggaa cagcggcagg ctcgacaggt
     3001 tcatcccgtt cgatgtcgac gaatccacac ttcgatcggc agcgctaggc cttgtctcct
     3061 cgtatcccga ccttcgggtc catggagtcg tcggcgactt cgaagagaac cttcaccaac
     3121 tacctaagga gggcaagcgg ctgatcgcct tcatcggcag cacgatcgga aatctcgatg
     3181 ccctcggccg gatgaagttc ctggaacaac tggcgggaaa catggctcct ggcgactact
     3241 tcctggtggg catcgaccta gtcaaagagc gagcaaccct cgaggtcgcc tacaacgact
     3301 ccgctggaat aactgctgag ttcaaccgca acatccttga cgttctgaac aacagtctcg
     3361 acggagactt cgcccccgac aggttcgagc atgttgcttt cttcgaccaa gacaactcct
     3421 ggatcgagat gcgacttcga tcgcgccagc agcaagaagt tcgcatcgca gcgatcgact
     3481 tggaactgaa cctatcctca ggcgaggaga tcagaaccga gatcagctgc aaattcacca
     3541 tcgagcaggt cgagggggaa ctccagcgat ccggactgat gcccctcgag tggtggctcg
     3601 acgataacgg gcggttcgcg ctggggctct ccgccaaacc ctagggagta gaaacgcccc
     3661 atttgcactg gttagccctg acggggctat gccccatatg gtccatctgc gctatccacc
     3721 ttgaaggcag gattaccgag aacagatgac gtgccaccta atgatccgtt actcatgcga
     3781 ttcatcggtg atgccactgc cgcatcactc ttcctgatgg cggccgctgc gatagccaac
     3841 tacgttagga caagggcgcg cagagaacgc accatcgcgt cgatcctcgc actgctcacg
     3901 atcacatcat catgcgaccg gctcctacgc cacctcggat ttgactggac catcgctgcc
     3961 ctggcctatg gcacattcat gctcgcgggc tatcagatcc tgctatttcg cgtcgacatc
     4021 ttccctatcc gacgccggat acaaccttgg gcccttctcg taacgctcgc aacaacagca
     4081 ttgggagcga ccttctcgat atccgaggaa ggaccacata ctccggccgg ggtggcaagt
     4141 ctcgccctat ctggagtgtt gtggatcatc gtcctcggcg agtcgtgcct cacgttctgg
     4201 cgtgcatcac gtcacaggca actgatccaa cgtgcgaggc tccgtgcctt ggtattcggc
     4261 tacctcgcgc tcatggtcct gctgtccgtt cgattcatcg ataccaacgc tcctggtgtc
     4321 gaccttctag ccgctccgct tcctttcttt ctctacatgg gcctgtcggc ccccgcgtgg
     4381 ttgagacggg cgtggcgtcg ccacgaggag gacctccttc cgatggtggt agacctcatc
     4441 agtcataccg acgatgtcgc ggagatggcc tctgcggggc tcgatagtgc cctaaggcta
     4501 acgggggcct cccacgcgac gctcatctgg agcgatggca tcatggcatc ctcctcgcaa
     4561 cctcgaaacg ctggcaccaa ccacaccaag ttggccgagt tggacctgcg gatggaacag
     4621 ggaaccgcga cactcgtgtt gctgcgcgga cctctcacgc ccgaactcga agaggacggg
     4681 agagcccgac tcactcgctt cgccaaatcc ctggcgatcg gccttgagcg tgcgatgctg
     4741 atggatcgag ctcggcagga agaagagagc gaagcagaga tcctgcgcga gcgagcaagg
     4801 atcgcagacc aactcgcact cgctcttacc atcgaacgct ctgcccatga ggaggtcgaa
     4861 gagctcgagc gcgttcgttc ggacttcatc gccagcgttt cgcacgaact ccgaacccca
     4921 gccacggtga tcaaggggtt cgcagcaacc cttatgcaga gctgggaccg actatcggac
     4981 gaagagcgca gaagtttcgt cgagatcatc agatcgagaa gcgacgacct cgaagaacta
     5041 ctggatgaac tcttggactt caccagatgg gagggtggtc atgccgagat ccaccaagac
     5101 ctcacaaagc cgaaggagtg cctcgatgaa gtggtctccg ctcttgggcc gggacaggcc
     5161 gctcgggtcc gcaatctgat ctccgatgac gtggtggtcg tgatggcctc ttcggccctg
     5221 cggcgagtcc tgagcaacct ggtctccaac gccctcaagt tctccgacga gtcggcccct
     5281 gtcctggtat cggccgtgga cgatggccac aaggttgagt tcgccgtgaa agatcaaggg
     5341 gtcggtgtag accctgcgga gatggccctg atattcgagc gcttctaccg cgtgaatcgg
     5401 accgactcaa cggcgagcgg gaccggcatc gggcttactc tcgtcaaggg gttcgtcgag
     5461 gcgggcgggg gccgggtctg ggcggagagt ctcccggggg ccgggagcac cttcttcttc
     5521 tctctcccat cccagggggg atcggaaccg tctcacaaga tggtgccggg gacagcagga
     5581 atgacgtcaa aagcgtagaa cccttcctta gtggcccaaa ccgggcacga aagagaaagg
     5641 ttcgaccatg tctcctcgac acacacctcg tggacccctg cgcttcctag ctgtggcggc
     5701 cgctctaggg ctcatcgcgg tcatcttccc ttcagccgga ctggcgacgc tgaccggctt
     5761 gcgagccggg gccgccagac tcgactcgac ctggcatgtg ggtgcctctg caggtcagta
     5821 cgcatcagat ggggtcgccg ttggagacca cggggtcgat ccgttcacgc actcaacacg
     5881 acggagtccc tcatatggga tacagggccg ggagtgggtc agagcgctcg ttgtggaagg
     5941 tagcgatgga aagcgtttcg ccgtggcatc taacgaccta tacatcccac aggatctgct
     6001 gaaccgcaga gttgcattga tcctcgcgga acacgacgcc aaggtccgtc tcggactcga
     6061 ggatggtcca gtgacgcgca tcagcgccga gaacctcacg atctcggtga gccacagtca
     6121 ctcctctcct tactactcga cgccctcctg gggcgtctgg gccttccagg atgtcttcga
     6181 catccgattc ttcgaacacc tttctcagaa gatggcggac gcagtgatcg cagctacggc
     6241 tagcctgcgg cctgctcgcg tcgggggagc ggtggggccc ttctcactgc tcaaacggca
     6301 ctcgttcggc ccccaggggg ccaacgacgg gacccccgcc ggataccccc agaccgacat
     6361 cgacccggac ctctcattgg tccgcttcga cgatgtgacg gacccacaga acccactgcc
     6421 tcttgcgaac tgggttatct tcggccttca ccccgagctg ttgagcggga acgacctatt
     6481 ggccaacgag tatgtgaacc ggatggagac gatcatcgat cgcgagatcg ggggcgtaac
     6541 tctcttctca cagaacgaca ccggaacatc ggaacctgca cgcaaccgtg acgctcacac
     6601 cgcggctcag agacaagagt tcagccatcg cgagtatgga caggtcgagc gcgctgctcg
     6661 gacccttgcc agttcggtag aagcccttca cgcggacatc gcaaccgttt cttcgaacaa
     6721 cccagcgacc tacgccaaca atgtggtgag gttcgcgtca gacgtgccca tcagattcaa
     6781 agacctgcgg ttcgctccgc cctcgtatcg gctgctccca actgtttcga gctgccgtac
     6841 cgagaaggcc ttcgacggca accctggggt cccgatcgtt gggctgcccg actgcgcctt
     6901 cctgtggaac gagacgatcg acgaagacaa agcccccggg tgggaccagc tcccgcagga
     6961 tccacaggtc acctatcgca cacttcgcga tgctggggtc cccatccccg acaacgtgag
     7021 cgccccctca tacacaggcc ttcaggagac ccttcaggtc caccttcagg ccatccgcat
     7081 cggggatatc ggtatcacgg tctgcccatg cgagcagtgg gccgaccagg cgcgcaacat
     7141 caagagcaga ctgaatcaga ccaacggtga cttctggtac ggctgggact ggacatcgaa
     7201 ctacacgcag agcggatgga cagcagggac gaactatgcc aacacggtcc tcggctggtg
     7261 cgacccgagc ggagcttcat ggatctgcaa ggacccaagg tcaccgagca cgaacctggc
     7321 cccgatcagt gactacacac tgaagcgctt caaggcccag atctacaacg acgccaaggg
     7381 atgggatgcc ctcgattaca cgacccagtc cgagagcgaa cccacagaca tatcgaagat
     7441 caagggcaac tacacgcatg aggagctgga cggggtcatg gccggcggtg gttacccgat
     7501 ggtgatcacg gtgggcatga gcaatgacta ctggggttac atcgcgacct accgcgagtt
     7561 ccagcgggga gatgcgtatc gcaaggccct gacggggctc ggcccccact catccgactt
     7621 cctcgccaca cgtctttcgc ggatggctgc cgagttgaag ggcggtccga aggtaaggct
     7681 cacggcgaag gacctcgcct accttcccga atacgcgcat caaggaagca gggccgaggt
     7741 cataggccag gcagcacaca actacgttgg tgcatacgag gctctgctgc ctgctgacgg
     7801 cggggccccc gcgatagtga cccagccctc gaacatcaag cgattctcgg ctgcgaaggt
     7861 ctcgtgggtc gggggctcga acttcacaga cacgcctcat gcgaaggtcg aacgctgcgc
     7921 tctggtggcc tgcgatgccg ccattgacac gggatgggtt cctttcggca atgggttcgg
     7981 tgaggtgcag gtgaaggcca actatccgca gccggaggat atgcctgcgt tcacggcggg
     8041 ccagttcgag tggcgctggg atgccacgtt cgaggccttc gactccgacg ttcctttgcc
     8101 cgacgcgtca gggacggctc gcactcagac ccctgccggt cagtacaggt tcgtcatcga
     8161 tgggtgctct cgctccccgt tcccaggagc ggccgacacg acctgcggga gttacgaccc
     8221 tggccacagg gtgcagccct accacttgcg ctcgggctcc ttcaccgtgt tgccgtggga
     8281 cggcatgacg attccctcga tatcgtcaga gcctggaggc atctcgttca ctgtcggccc
     8341 cgcctacccc tgggcgataa cagcatcaaa cacagagcgg gggccgatcg actatccgaa
     8401 cagctatgcg agcctcttca agttcatcgc caaccgcagc gccagtgatg tcttctacgg
     8461 cgggtcggag gtcttctgcc taaagtgttc gttccgaccg tgggccgaca ccggttcggt
     8521 cgcaacagcc aaggtgaccg tcgtacgctc aggagtgcct gaactcgtcg atgcgactct
     8581 gggactcgat ggcaggtggc acgccgcagt caccctggca gcaggggacg aggcctacat
     8641 cgcccccggc gggatagtcg atgagttcgg cgagtacaac ggatcggaga gcgcccacgt
     8701 agttgggtag ctactacagt tcttttggt
//
LOCUS       DATDOZ010000272         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2341765, whole genome shotgun sequence.
ACCESSION   DATDOZ010000272 DATDOZ010000000
VERSION     DATDOZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341765"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VI541_05495"
     CDS             complement(<1..1029)
                     /locus_tag="VI541_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HLF70393.1"
                     /translation="MNALIYLRVSTKEQATKDETAEGYSIPAQREACIRYISEKGWSV
                     ADEFIDAGESARSADRPNLKEMLRRVGEGDITAIVVHKIDRLARNIEDHVAIRAVLRK
                     HGCQLASVTENIEETASGKLVEGIHALMAEFYSSNLASEIRKGMSQKAKMGGWPQRAP
                     IGYLNQREKTGGKEIARIILDPQRAFLVKEAFRLYATGDYSLSELHSTMEAKGLTSIA
                     SKSGKPPVISKLAEMLANPFYIGIVEWNGVRYQGQHKPLISRALFDKVQEVLRLHNKA
                     GVRQRSHDHYLKGLLHCGECGRRLSLTLAKGTYLYFYCLGQKNALRKQTNCTQPYVMT
                     CDAEVLIEN"
     gene            complement(1026..1211)
                     /locus_tag="VI541_05500"
     CDS             complement(1026..1211)
                     /locus_tag="VI541_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70394.1"
                     /translation="MPERGSATGSDARVSASRRRLASVGDLEELKDSALRLFASTLID
                     LALSLIEEAEEEKEAAA"
     gene            complement(1204..2223)
                     /locus_tag="VI541_05505"
     CDS             complement(1204..2223)
                     /locus_tag="VI541_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HLF70395.1"
                     /translation="MSARQEQQAVTIGRLLDQRDREILLALFEHRLLSTSQLMVLFFS
                     SLRRCQDALKKICDLHLIERDRPFQQVGTGKDESLWTLTEAGVRIVAVTMRKARSQLD
                     WMPRFSFHVSDRQLDHLLGVNRFFVSLVEATFVHPGHGLEKWVVAKQVKSQNNWVTHD
                     GFGRYQHQGGACDFYFEYDRGTEWHDQLVRKLRGYVLMAIKWTEEGPDHFPNVLVLVP
                     DEKREKAFDRAMKAAVESLQIAEQIAEKVAVSLPMFITSEELLSQSGMLGKVWRSFAP
                     PPANRPLAPLFMAVRQSLTELPAHKAGPFDLERCLGKRWSDAGARSKLRRRIPPTFPA
                     GDLDA"
BASE COUNT          460 a          742 c          682 g          531 t
ORIGIN      
        1 gttctcgatc agcacctctg catcgcaggt catgacgtaa ggctgcgtgc agttggtttg
       61 cttccgcagc gcgttctttt ggccgaggca gtagaaatag agataggtcc ccttcgccaa
      121 agtaagcgac agccgtcggc cgcactcgcc gcagtgaaga agtcccttga ggtagtggtc
      181 gtgggaacgc tgcctcactc cagccttatt gtgtagccga agaacctctt ggaccttgtc
      241 gaagagggct cttgagatga gaggcttgtg ctggccttgg tagcggacgc cgttccactc
      301 gactatcccg atgtagaagg gattggctag catctccgcg agcttgctaa tgaccggggg
      361 cttacctgac ttggaagcga tcgaagtaag tcccttcgct tccatggtcg agtgaagctc
      421 cgagagcgag taatccccgg tcgcgtagag tcggaacgct tccttcacga ggaacgcgcg
      481 ctgtggatcg aggatgatcc gtgcgatctc ctttccaccg gtcttctctc gctggttcaa
      541 gtagccgatc ggtgcccgct gaggccagcc tcccatcttc gctttctggc tcatgccctt
      601 gcggatctca gaggcgaggt tggacgagta gaactcagcc atcaacgcat ggatcccctc
      661 gaccaacttt cccgatgcgg tctcttcgat gttctctgtg acggatgcga gttggcaacc
      721 gtgcttccta agcactgccc ttatcgcgac gtggtcttcg atgtttctgg cgaggcggtc
      781 gatcttgtga acgacgatcg cggtgatgtc cccttcaccg actcgccgca gcatctcttt
      841 aagattcggc cggtctgctg agcgcgccga ctccccggcg tcgatgaact cgtcggcgac
      901 gctccagcct ttctcggaga tgtatcggat acacgcttca cgctgtgccg ggatcgagta
      961 gccctcggcc gtctcgtctt tggttgcctg ctctttggtt gagactcgca gatagatcaa
     1021 tgcgttcaag ccgccgcctc cttctcctct tcggcttcct caatgagcga gagagccagg
     1081 tcgatcaaag tcgatgcgaa caatcgcaac gcactatcct tgagctcttc gagatccccc
     1141 acagacgcga gcctacgacg cgacgccgac acccttgcat ccgaccctgt cgccgagccc
     1201 ctctcaggca tccaagtccc cggccggaaa ggttggcggg atgcgccgcc gcagcttcga
     1261 gcgggcccct gcatcgctcc atctcttgcc aaggcagcgt tcgaggtcga agggtccggc
     1321 tttgtgggcc gggagctcag ttagcgattg gcgcacggcc atgaacaaag gggcaagtgg
     1381 cctattcgca ggagggggag cgaaggacct ccaaaccttt cccagcatcc ccgactgaga
     1441 gagcaactcc tcggacgtga tgaacatcgg caaagagacc gcgaccttct ccgcgatctg
     1501 ctccgcgatc tgcaacgatt cgaccgccgc cttcatcgcc cggtcgaagg ccttctcacg
     1561 cttctcgtcg ggcaccagga cgagaacgtt ggggaagtga tctggcccct cctcggtcca
     1621 cttgatagcc atcaggacgt acccacgaag ttttcgaacc aactggtcgt gccactcggt
     1681 gcctcggtcg tactcgaagt agaagtcgca ggcgccgcct tggtgttgat atcgaccgaa
     1741 cccatcgtgg gtgacccagt tgttctgact cttgacctgc ttggcaacca cccacttctc
     1801 gaggccatga ccgggatgaa caaatgtcgc ctcgacaaga gagacgaaga acctgttcac
     1861 gccgagcagg tgatcgagct gccggtcgga gacgtggaag ctgaaccttg gcatccagtc
     1921 gagctgagaa cgagccttac gcatagttac cgccacgatc cggaccccgg cctcagtcag
     1981 tgtccagagt gattcgtcct tccccgtacc gacctgctgg aagggccgat ctctttcgat
     2041 caagtgaagg tcgcagatct tcttgagtgc atcctgacat ctgcgaagtg acgagaagaa
     2101 caacaccatc aactgcgatg tcgaaagcag ccgatgctcg aagagagcga gcaggatctc
     2161 gcggtcgcgt tgatcgagga ggcgaccaat cgtgactgcc tgctgttctt gtcgtgcgct
     2221 catgtcgtca gtgtctctcc tgtcgtttgc gtccctctcg tctcatccaa taaccctgcg
     2281 tttcgtgttg ttgggagggc aaagccctcc cccctccttc gccatcggag gcctggggca
     2341 accggcacat gagctggtgg ttttgccaag tcatcgcctt agcccagcgc cacgaaaacg
     2401 ccattcgtct cctcg
//
LOCUS       DATDOZ010000273         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2581966, whole genome shotgun sequence.
ACCESSION   DATDOZ010000273 DATDOZ010000000
VERSION     DATDOZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581966"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="VI541_05510"
     CDS             complement(<1..1815)
                     /locus_tag="VI541_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLF70396.1"
                     /translation="MHEVIWKPSHAYIEGSNLERLMRSQGIDTFEDLVSRSIDDIEWY
                     WSAAEKDLGLQWIRPYDRVLDSRQGIEWTTWFVGGQINIADNCVDRHVSGGRAAKTAL
                     TWEGEDGSTRSVTYRQLADEVGRFSEGLRSLGIGKGDAVGVYMPMSIEAVVAMLAVAK
                     VGAIYLPIFSGFAAHAVAVRLQDAGAKLLVTADGFYRRGQKIPMKTVAREAAEAAHVE
                     QVIVWSRFGLDREFREGEFDWDDLVSRQPAASATEPTDAEDVWMIAYTSGTTGKPKGS
                     VHVHGGFLVKIASEVAYQVDLREDDVLYWVTDMGWIMGQWETVGGLANGGSVVLYEGA
                     PNFPEPDRVWDVCERHGVTILGISPTLVRALIPSGVEQVDKHDLSKLRILGSTGEPWN
                     PEPYLWFFNNIGKGRCPIINLSGGTEVGACFLSPYPISDIKPVSLKGPSLGMAVDVVD
                     TEGRSIRGDVGELICRKPWPGMTRGIWGDRDRYIEAYWSRFPGMWCHGDWASVDADGH
                     WFLHGRSDDTLNIAGKRIGPAEFESVLVAHPLVMESAAVGVEHSVKGETVWCFCVLTP
                     GAQASDELSKDLSAMIDAEIGKAFRPERIFFVPELPRTR"
     gene            complement(1839..2513)
                     /locus_tag="VI541_05515"
     CDS             complement(1839..2513)
                     /locus_tag="VI541_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HLF70397.1"
                     /translation="MDLLTPELEILGRLPYSSNYTFLATLKGSDERVVYKPRDGESPL
                     WDFPPGSLCQREVAAYLVAEELGWGLVPPTVLRDGPLGIGAVQSFIEHDPSITAFSLG
                     PRHIPELRRVALLDVVINNADRKGGHVILDVDDRVWSVDHGICFHEEPKLRTVIWVFA
                     GEQVTEEEMHDLQGLEGSLGAIGGLAELLSPSELRALEGRLGGLIESGVFPQPGPGRS
                     TPWPPV"
     gene            complement(2521..3021)
                     /locus_tag="VI541_05520"
     CDS             complement(2521..3021)
                     /locus_tag="VI541_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HLF70398.1"
                     /translation="MSDELPDSVVVGAVGEPGQRTFLLQVRQSGTLITVIVEKQQVAE
                     LSQVAFALLAELGQLRLAQELIRSEGGVPLPHFEADEPLFRVSSFELGFDSDANLVIL
                     AARPDEDDVEPLRLQLSLTELGRLATQGAESVARGRPICPICGLPMEADGHICPAVNG
                     HRPRTA"
     gene            complement(3018..>3648)
                     /locus_tag="VI541_05525"
     CDS             complement(3018..>3648)
                     /locus_tag="VI541_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLF70399.1"
                     /translation="LLYLVRHGLTSITGKKLTGWMPGISLSDEGIAQARAAAERLADV
                     DLSAVYSSPLERCVETAREVAVPRKMKVRTLPGVGEVRYGDWQGKTFVTLYKSRAWKE
                     LKARPADFRFPNGETIREAQTRGMTTIEGLRGKHRGEAVLVCSHADLIRVVVAGYLGL
                     PLDLYDRITIAPASTTVLLLGDVPRLIKLGETGDHTAEIKRLKASGAKR"
BASE COUNT          725 a         1198 c         1048 g          677 t
ORIGIN      
        1 gcgagtgcgg ggcagttctg ggacgaagaa gatccgttcg gggcggaagg ccttacctat
       61 ctctgcatcg atcatcgcgc tcagatcctt ggacagctcg tcggacgcct gcgcccccgg
      121 agtcagcaca cagaaacacc agacggtctc gcccttcacg gagtgctcca ccccgacagc
      181 tgccgactcc atcaccaacg gatgagcgac aaggacagac tcgaactcgg caggacctat
      241 ccgcttgcca gctatgttga gcgtgtcgtc gctgcgccca tggaggaacc agtgaccatc
      301 ggcgtcgacc gaggcccagt cgccatgaca ccacatgccg gggaagcgcg accagtaggc
      361 ctcgatgtat ctatcgcgat caccccatat cccgcgggtc atcccgggcc acggcttgcg
      421 gcagatcagc tcaccgacat cgccccggat cgatcggccc tccgtgtcga cgacatcgac
      481 ggccatccca agtgaggggc ccttgagcga caccggcttg atgtcgctga tcggatacgg
      541 cgacaggaaa cacgcaccga cctctgtgcc gccagagaga ttgatgatcg gacatctccc
      601 cttgccgatg ttgttgaaga accacaggta gggttcgggg ttccagggct ctccggtgga
      661 cccgaggatg cgcaacttcg agaggtcgtg cttatcgacc tgctcgactc ccgaagggat
      721 caatgcccgc accagcgtcg gtgagatgcc caggatggtc acgccatgtc gctcgcacac
      781 atcccacacc cggtcgggct cggggaagtt gggggccccc tcgtacagaa cgacgctacc
      841 cccgttggca aggcccccca cggtctccca ctggcccatg atccagccca tgtccgtcac
      901 ccagtacagg acgtcgtcct cacgaagatc cacctggtac gcaacctccg atgcgatctt
      961 gacaaggaaa ccgccgtgaa catgcaccga ccccttcggc ttaccggtcg taccggatgt
     1021 gtaagcgatc atccatacgt cttcggcatc ggtgggttcc gtggcggacg cggccggttg
     1081 acgcgagacg aggtcatccc aatcgaactc gccttcccga aactcccggt cgaggccgaa
     1141 tctggaccag acgatcacct gttccacgtg agctgcttcg gcagcctctc gagccaccgt
     1201 tttcatcggg atcttctgac cgcgtcggta gaaaccatca gcggtgacca gaagtttggc
     1261 cccagcgtcc tgcagccgaa ccgcgacggc gtgcgcagcg aaccccgaga agatggggag
     1321 atagatggcc ccaaccttgg cgacggcaag catcgcaaca actgcctcga tcgacatcgg
     1381 catgtatacg ccgaccgcgt ctcctttacc gatgcccagt gacctcaaac cctccgagaa
     1441 acgcccgact tcgtccgcga gttgccgata tgtcaccgac cgggtcgaac catcctcgcc
     1501 ctcccaagtg agggcagtct tcgcggcgcg gcccccagag acgtgacggt caacgcagtt
     1561 gtcggcgata tttatctggc cgccgacgaa ccaggtggtc cactcgatgc cctggcgaga
     1621 gtcgaggaca cggtcataag gccttatcca ctggagtccg aggtccttct cggccgctga
     1681 ccagtaccac tcgatgtcat cgatggatcg tgaaaccagg tcctcgaatg tatctatgcc
     1741 ctggctgcgc atgagccgct cgaggttcga gccctcgatg taggcatggc ttggcttcca
     1801 tatgacctca tgcatcagtg cattctacga gccagcattc atacgggggg ccatggggtc
     1861 gagcggcccg gtcctggttg tgggaagacc cccgactcga tgagtccgcc caaacggccc
     1921 tccaaggccc tcaactccga tggactgaga agttctgcga ggcccccgat cgcaccgaga
     1981 gatccttcga gcccctgaag gtcgtgcatc tcttcttcgg tcacctgctc acctgcgaag
     2041 acccagatca ccgttcgcag tttcggctct tcgtggaaac agatcccatg atccaccgac
     2101 cagaccctgt cgtcgacatc gaggatgacg tgtccgccct tcctgtccgc attgttgatc
     2161 acgacgtcta gcagggcgac cctgcgaagt tccggtatgt gacgggggcc cagcgagaac
     2221 gctgtgatgc tgggatcgtg ctcgatgaaa ctctgaactg caccgatccc caacggacca
     2281 tccctcagaa ctgtcggtgg aacgagcccc catccgagct cttctgcgac aaggtaggcc
     2341 gcaacctcgc gttgacagag tgatcctggc gggaagtccc acagaggtga ctcaccatcg
     2401 cgcggtttgt aaacgacgcg ctcatcagaa cccttgagtg tcgcgagaaa ggtgtaattc
     2461 gatgaatacg gcaggcggcc gaggatctct agctcgggcg tcagcagatc cacccgcgga
     2521 tcacgccgtg cgggggcgat gtccattgac cgcagggcag atgtgaccgt cggcctccat
     2581 gggaagacca cagatcgggc agatcggacg tcctcgcgcc accgattcgg ccccctgggt
     2641 cgcgagcctt cccaactccg tcaacgagag ttgcagtcgc agtggctcca catcgtcctc
     2701 gtcgggccgg gcggccagta tcaccaggtt ggcatcactg tcgaagccaa gctcgaaaga
     2761 gctgaccctg aacagcggtt cgtcggcctc gaaatgcggg agcggaactc ccccctcaga
     2821 gcggatcaac tcctgagcca accgaagctg tcccaactcg gcgagcaagg cgaacgcgac
     2881 ctggctcaac tcggcgacct gctgtttctc gactatcacg gtgatcagtg ttcccgactg
     2941 gcggacctgt agcaggaagg tccgctgtcc gggctcgcct accgctccga ctacaacgct
     3001 atcgggcagt tcatcgctca tcgtttcgcc cccgatgcct tcaatcgttt gatctctgcc
     3061 gtgtgatcgc cggtctcacc gagcttgatg agtctcggaa cgtcacccaa gagcaggacg
     3121 gtagtggaag caggagcgat ggtgatgcga tcgtagaggt caagagggag gcccagatac
     3181 cccgcaacca cgacccttat caggtcagcg tgtgaacaga cgagcaccgc ctcaccacga
     3241 tgtttgccgc gcagaccttc gatagtcgtc atacctctcg tctgggcttc gcggatggtt
     3301 tcgccattgg ggaaccggaa gtccgcgggc ctggccttga gttccttcca agcgcgggac
     3361 ttgtacagag tgacgaacgt cttgccctgc cagtcgccat atcggacctc gccgactccg
     3421 ggcagcgttc gtaccttcat cttgcgaggg acagccactt ctcttgccgt ttccacgcac
     3481 ctctcgagcg gactcgagta gacggcgctg agatccacgt ctgccaacct ctccgccgcg
     3541 gcgcgagcct gagctatccc ctcgtcggac agggagatcc ccggcatcca cccggtgagc
     3601 ttcttgccgg ttatcgaggt caggccgtga cgaacgaggt acagaagc
//
LOCUS       DATDOZ010000274         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_748441, whole genome shotgun sequence.
ACCESSION   DATDOZ010000274 DATDOZ010000000
VERSION     DATDOZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748441"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VI541_05530"
     CDS             complement(<1..1006)
                     /locus_tag="VI541_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLF70400.1"
                     /translation="MTALLVDEDGVPTRKAYREFPSYFPQPGWVEQDPDEIWRTVVET
                     VAEVTAGTAPSDIAAVGITNQRETTVLWERSTLNPVHRAIVWQCRRSAPICEELRERG
                     VESEIRRRTGLLLDPYFSGTKLTWLFRNNPEFATRAAKGELAFGTIDSWLIARLTDGA
                     LHATDPSNASRTLLYNLAEARWDPYLLELMEVPEAVLPKVEPSSHRFGGCTLPSLAGV
                     PIGGVAGDQQSALFGQACFTKGMAKNTYGTGSFILTNAGAEAPVSTHGLLTSVAWEIE
                     SRIEYALEGSVFITGAGIQWLRDGLGIIGSSAEAGPLASSVEDSAGVYFVPALTGLGA
                     P"
     gene            complement(1090..2127)
                     /locus_tag="VI541_05535"
     CDS             complement(1090..2127)
                     /locus_tag="VI541_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017295031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLF70401.1"
                     /translation="MDRKLRMTLALMMVLLSSMFSPRDVAVASTSQERVGALSKRLGS
                     LIAQMNRLEAQATAAQGRVGVSNYLIDRARQRQSLNRSALEARVREAYMRGIWPEANL
                     VFGLRGLREVLNFSRYLGESMAQESEVMKRYVEAEATLARTRSGAASEESTLNESLAM
                     LQKLRNQVQVALDSESRILASSRAEIARLKARRAVSDYGVSLIVTQRRIARQRVLDEK
                     LAALIEWYSPAAGTEPFMPPKLTSTGVVTTGRASWYGPGFDGRRASSGATYHQEQMTA
                     ASLVLPFGTLLKVTFRGRSVVVVITDRGPYVAGLVLDLSKGASAAIGHSGVQTVTMEI
                     LTPTEPAPAFP"
     gene            2182..>2462
                     /locus_tag="VI541_05540"
     CDS             2182..>2462
                     /locus_tag="VI541_05540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HLF70402.1"
                     /translation="MHVGVLTGGGDCPGLNAVIRAIVRKGIDRYGLAILGFRDGWQGV
                     MEGHAEELTVQNTRGILHRGGTILGTSRTNPYKSAEGPQQVLDHIEAHR"
BASE COUNT          472 a          753 c          770 g          467 t
ORIGIN      
        1 ggggggcccc aagccctgtt agtgctggca cgaaatagac accagcggag tcctcgacgc
       61 tcgaggcgag ggggccagcc tcggcggaag atccgatgat cccgagtccg tcgcggagcc
      121 actggatacc tgcgccggtg ataaagacag aaccttcgag cgcgtactcg atgcgggact
      181 cgatctccca ggcgaccgat gtgaggagcc catgtgtgct gaccggtgct tctgcccctg
      241 cattggtgag gatgaatgac cccgtgccat atgtgttctt ggccatgccc ttggtgaagc
      301 aagcttggcc aaagagtgcg gactgctgat cacctgccac accaccgatc ggaacgcccg
      361 ctagcgaagg aagcgtgcat ccgccgaacc gatgcgacga aggctcgacc tttggaagga
      421 ccgcctcggg aacctccatc agttcgagca ggtaggggtc ccaccgcgct tcggcaaggt
      481 tgtagagcag cgtcctcgac gcgttcgagg gatcggtggc atgtaaggcc ccatcagtca
      541 gtcttgcgat gagccatgag tcgatagttc cgaatgcgag ctcgcccttg gcggctcgcg
      601 tcgcaaattc agggttgttg cgaaagagcc atgttagctt cgtgcccgag aagtaggggt
      661 cgagcaggag ccccgttctc cgacggatct cggactcgac accgcgctct cgcaactctt
      721 cgcagatggg agccgacctt cggcactgcc acacgattgc tcggtgtacc ggattcagag
      781 tcgaacgctc ccacaggacc gtcgtctcac gttgattggt tatgccgacg gctgcgatgt
      841 cggaaggggc ggtccctgct gtcacctcag ccacggtctc gaccaccgtc cgccagatct
      901 cgtctgggtc ctgctcgacc cagcctggtt gggggaagta ggaggggaac tcccggtatg
      961 ccttgcgagt gggcactcca tcttcatcga cgaggagcgc cgtcaccccc gttgtccccg
     1021 cgtcgatagc taccaccgaa cttgggcgaa taagcgtcgc ataccggtgg tttttcgccc
     1081 aagttggggt catgggaaag cgggggctgg ttcggtcggg gtcaggatct ccatcgtcac
     1141 ggtctggacc cccgagtgtc cgatggctgc ggacgctccc ttcgacaggt cgagcaccag
     1201 accggcgaca tacggccccc gatcggttat gacgacgacc acgctgcggc ctcggaaggt
     1261 gaccttcagc agagtgccga agggaagtac gaggctggcc gcggtcatct gctcctgatg
     1321 ataggtagcg ccgctggatg cgcgtcggcc atcgaaaccg gggccgtacc atgacgcacg
     1381 ccccgtcgtg acgacaccgg tagatgtcag tttcgggggc atgaacggct ctgtccccgc
     1441 tgctggcgag taccactcga tcaaggcggc gagtttctcg tcgagaactc gctgtcgtgc
     1501 gatacggcgc tgagtgacga taagtgaaac accgtagtcg ctcacggctc tgcgggcctt
     1561 gagccgggcg atctcggccc tcgatgaggc aagtatccgt gactcactgt ccagtgctac
     1621 ctgaacctgg ttgcgaagtt tctgcaacat cgccagagat tcgttcaggg tggattcctc
     1681 agatgccgcc cccgaacgcg tgcgagccag agtggcctca gcctctacat accgcttcat
     1741 cacctcggac tcctgcgcca tcgactcgcc caggtaccgg ctgaagttca ggacttcacg
     1801 caacccccgc agaccgaaca cgagattagc ttcgggccag atgccgcgca tgtaggcctc
     1861 cctgaccctg gcttccagcg cggagcggtt caaagactgc cgctgcctcg ctctgtcgat
     1921 gaggtagttc gacacaccaa ctcggccctg agcggcggtt gcctgagctt ccaatctgtt
     1981 catctgagcg atgagtgaac cgaggcgttt ggacagggct ccgacccgct cctgcgatgt
     2041 ggacgccacc gccacgtcgc gcggcgagaa catcgatgac agcagcacca tcatcagggc
     2101 cagagtcatg cgcagtttgc gatccatggg cctgaaatcc tagtcgaggg ggccgccatt
     2161 tcacggatag aatgcggcta catgcatgtt ggtgtgctca ccggtggtgg tgactgtcca
     2221 gggctcaacg ccgtgatccg ggcgatagtc cgcaagggca tagatcgtta cggcctcgcc
     2281 atcttgggat tccgtgatgg ttggcaggga gtgatggaag gccatgccga ggaactcacc
     2341 gtccagaaca caaggggcat cttgcatcgg ggtggcacga tcctgggcac ctcgcggacc
     2401 aacccctaca agagcgccga gggaccacag caggtccttg accatataga ggcgcatcgc
     2461 at
//
LOCUS       DATDOZ010000275         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2584651, whole genome shotgun sequence.
ACCESSION   DATDOZ010000275 DATDOZ010000000
VERSION     DATDOZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584651"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VI541_05545"
     CDS             complement(<1..1199)
                     /locus_tag="VI541_05545"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HLF70403.1"
                     /translation="MPTKPKTPARPKTKSKARAAKPKAAARAPISKPDQNAPPEPHRI
                     PVESRIEELRKRKLEALEGGGPSAIERQHERGKLTARERLDILLDKDSFVETDMLVRH
                     RASGFGIEDRRPHGDGVVTGWGTIDGRKVFVFSQDFTVFGGSLGEAYAEKICKVMDLA
                     MSTGAPLIGINDSGGARIQEGTPSLAGYAHIFDRNVRASGIIPQISVILGPCAGGAVY
                     SPAMTDFIFMTEDTSHMAITGPEVIKAVTGEDVTLEELGGATTHSTRTGIAQFVGSTE
                     QQTLEMVRYLVSFIPSNNLDDPPYFPPSDAPDRAEAALTELMPDASNKPYDMHQVIKL
                     VFDDAEFFEVFPNWAQNLITGFARLNGRSVGIVANQPQVLAGVLNIDASIKGARFVRF
                     CDAFNIPL"
     gene            complement(1210..2112)
                     /locus_tag="VI541_05550"
     CDS             complement(1210..2112)
                     /locus_tag="VI541_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70404.1"
                     /translation="MTVEFTESDSEVFRRFIATEYAYFTPKGEPLCWPVTPYWYPDRG
                     VLAIATGLAYPTKADYAALNPKVALLFSDPTGSGLPDESHILVQGDARVLSEDMQANA
                     DRYVTELRARFLSARIGINPLTVRLLDFYLPRLWVEIDPVAVERRKPNRPADGDLPRA
                     QGLRSNLDAKDVVALRNVVTRHGQGVVTIRGSDGYPQMLRTSVTMQDDLVLLRESPGT
                     GAACLTMHQHTLRGTRFSAYMVRGELVDAGEAHVFIPRRLVGFFGNGAVFPLSVIPSV
                     GRLRRRLGAELARRGKEMPKLRIP"
     gene            complement(2109..>2428)
                     /locus_tag="VI541_05555"
     CDS             complement(2109..>2428)
                     /locus_tag="VI541_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLF70405.1"
                     /translation="SANEAIATIALAPYTTTWGIAELEGDRIKGFAQSPRLPYWINGG
                     IYRFEPALTELLPDLGDHEDSTFPTLASQGRLYAFFIDGYWRGIDTAKDIIEATKEIE
                     RGQ"
BASE COUNT          425 a          708 c          809 g          486 t
ORIGIN      
        1 agcgggatgt tgaacgcatc acagaagcgc acgaagcggg ccccctttat cgaggcgtcg
       61 atgttgagaa cacccgcgag cacctgcggc tggttcgcga cgatcccgac cgatcttccg
      121 ttgagtcttg cgaatccagt aatcaagttc tgtgcccagt tcgggaagac ctcgaagaat
      181 tcggcgtcgt cgaagaccag cttgatcacc tggtgcatgt cgtatggctt gttagatgcg
      241 tcgggcatga gttccgttag ggcggcctcg gcccgatccg gcgcatccga tggcgggaag
      301 taaggcgggt catcgaggtt gttcgagggg atgaaggaga ccaggtagcg gaccatctcc
      361 agggtctgct gctcggttga tccgacgaac tgtgcgatcc cggtgcgggt cgagtgtgtc
      421 gtggcccccc caagttcttc gagggtcacg tcctcgcccg tcacggcctt gatgacctcg
      481 ggacctgtga tcgccatgtg cgatgtgtcc tcggtcatga agatgaagtc ggtcatggca
      541 ggggagtaga cggccccccc ggcgcagggg ccgaggatga cggatatctg agggatgatt
      601 cctgatgctc gtacgttgcg atcgaagatg tgcgcatagc ccgcgagtga gggtgttcct
      661 tcttggatgc gcgcgcctcc cgaatcgttg atgccgatca agggggcccc cgtcgacatc
      721 gccaggtcca tcaccttgca gatcttctcg gcgtatgcct cgcccagcga gccaccgaat
      781 acggtgaagt cctgcgagaa gacgaatacc ttgcgaccat ctatggtgcc ccaaccggta
      841 acgacgccgt caccgtgtgg tcggcggtcc tctatgccga accccgaagc tcgatggcgt
      901 accagcatgt ctgtctccac gaaggagtcc ttgtcgagga ggatgtcgag ccgctcacgc
      961 gccgtcagct tgccgcgttc gtgctgtcgc tcgatcgcag agggacctcc gccttcgagg
     1021 gcctcgagct tgcgtttgcg aagttcctcg atgcgcgact cgaccgggat cctgtgaggc
     1081 tccgggggcg cgttctggtc tggcttcgag atcggcgcgc gggccgcggc cttgggtttg
     1141 gcggctcttg ctttcgactt ggttttcgga cgagcaggtg tcttgggctt cgttggcatg
     1201 cgcgggagtc taagggatgc ggagcttggg catctccttg ccccgtcggg caagctcggc
     1261 ccccaaccgg cgacgcagcc gaccgacgct cgggatcacc gagagcggaa agacagcgcc
     1321 gttgccgaag aaaccgacga gccttcgcgg gatgaagaca tgcgcctcgc ccgcatccac
     1381 gagctctcct ctgaccatgt aggcgctgaa tcgtgtgccc cgcagcgtgt gctggtgcat
     1441 ggtgaggcac gcggcccccg tgccgggcga ctctcgaagg agcacgaggt catcctgcat
     1501 ggtcacggat gtgcgcagca tctgcgggta cccgtcactg ccacggatcg tgaccacccc
     1561 ctgaccgtgg cgcgtgacaa cgttgcggag tgccacgaca tccttagcgt cgaggttgga
     1621 ccgcaacccc tgggcgcgtg gaaggtctcc gtcggcaggg cggttcggct tgcgacgctc
     1681 caccgcgaca gggtcgatct cgacccagag ccgcggcagg tagaagtcca acaatcgaac
     1741 cgttagagga ttgatcccga tgcgggccga gaggaatctc gctctcagtt ctgtcacata
     1801 cctatctgca ttcgcctgca tgtcctcgga gaggactcga gcatcgccct gaaccaggat
     1861 gtgcgactcg tccggaaggc ccgaaccggt cggatcggag aagagcagag cgactttggg
     1921 gttgagcgcg gcgtagtccg ctttggttgg ataggccaga cctgttgcga tggcgaggac
     1981 ccctcggtcg ggataccagt acggagtgac gggccagcag aggggttcac ccttcggggt
     2041 gaagtaggca tactcggttg cgatgaaacg gcggaagacc tcggagtcgc tctcggtgaa
     2101 ctcgaccgtc attggcctct ctctatctcc ttcgtcgcct cgatgatgtc cttagcggtg
     2161 tcgatcccac gccagtaacc atcgatgaag aacgcataga ggcgaccctg cgatgcgagt
     2221 gtggggaagg tcgagtcctc gtggtctcct aggtcgggaa gcagttccgt gagggcaggt
     2281 tcgaatcggt agatgccccc gttgatccag taggggaggc gggggctctg cgcgaacccc
     2341 ttgatacggt cgccttcgag ttcggcgatg ccccatgtcg tcgtgtacgg agcgagggcg
     2401 atggtggcga tggcctcatt ggcagagt
//
LOCUS       DATDOZ010000276         3052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3616174, whole genome shotgun sequence.
ACCESSION   DATDOZ010000276 DATDOZ010000000
VERSION     DATDOZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616174"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /gene="pheT"
                     /locus_tag="VI541_05560"
     CDS             <1..1678
                     /gene="pheT"
                     /locus_tag="VI541_05560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLF70406.1"
                     /translation="GYRPVSNVVDATNYVLLVTGQPLHAFDLDKVGGSKILVRRAGSG
                     EKITTLDGEVRELDTDDLVIADSNVPVAIAGVMGGIETEVSAGTKRVMLESAHFDPRS
                     IARSAKRHGLRSEASARFERGSDPRNVEYPAALAASLMVDWAGGRVAAGNVVVGSAPE
                     LGQPIELRPERCRAILGFDISGDEMVDALTRLGLSPTLSDGVIRVARLSRRGDLQLEE
                     DLIEEVARLGGYDRVPSTLASGRYRAGSLTRAQILIRRIRSALAGAGLFEAYTPTFIG
                     PDDLAVLGIEDDHAWGGPLRVTNPLSRDESLLRTSLIPGLLQCVKTNISRRNLDIRLF
                     EIGRVFHGSDGQLPNEPERLSVVIAGTQRQEWLSEERELDLFDLKGALEVLFSSLRTP
                     DVRFVDSAGHPAHPTRSCLVTTGGRTLGVLGELDPTIAEAADLGIRVFFAEIELEALI
                     ETAADPQPTQQVWRFPAVLLDLAMVIEESIPIDAILGVVHSVGGDHLESARIFDVYRG
                     AQVESGYRSVAISLVFRNEERTMTESEAIEVRDRIAAELSSRFGAQVRGS"
     gene            complement(1721..2143)
                     /locus_tag="VI541_05565"
     CDS             complement(1721..2143)
                     /locus_tag="VI541_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70407.1"
                     /translation="MEVDYAFIAESADAQNGLFYVVRGGTDVWYVPAGSTYPLPIGPM
                     SLVIRLSGERSEIGRQLPVSFTIVDADGRPIGIEGNGEIVFGEHGVDRTRTGGALLHF
                     RLGFAVPAQGAFIFQLFSDGQRLCQVPFWIVESPNTPK"
     gene            2246..>3052
                     /gene="argC"
                     /locus_tag="VI541_05570"
     CDS             2246..>3052
                     /gene="argC"
                     /locus_tag="VI541_05570"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLF70408.1"
                     /translation="MAVRTAVLGASGFAGSELLRLLEGHPETQVIAATSSSRVGESLE
                     TMYPHLTKLAGLRFVSIEDALSLGVDVVFSSLPHGESGKLLARYQGRVVDLAGDFRLR
                     EATSYEPWYGEPHPYPSALPEWVYGLSEFSRDKIAGALKVANPGCYPTAALLALGPLV
                     AGQLVDASSIHIDAKSGVSGAGRAGGEGFDFSAMNENMRAYSPSGHKHIAEMEQELAV
                     MAGDTVTITFIPHLVPLTRGLLVTCIAPVADGVDAGTLDRAMADAYASERF"
BASE COUNT          556 a          922 c          971 g          603 t
ORIGIN      
        1 gggatatcga cctgtctcca acgtcgttga tgcgaccaac tacgtgctgc ttgtgacagg
       61 ccagcctctt catgccttcg acctagacaa ggtcggtgga tcgaagatcc tcgtgaggcg
      121 agccggatcg ggcgagaaga tcaccaccct cgatggtgag gtgcgcgaac tcgataccga
      181 cgacctggtg atcgccgact cgaatgtgcc ggtggccatc gcaggagtga tgggtgggat
      241 cgagaccgag gtctcggccg ggacgaagcg cgtgatgctc gagagtgccc acttcgaccc
      301 gcgctccatc gcccggtccg cgaaacgcca cgggcttcgc tcggaagctt cggcgcgatt
      361 cgagcgcgga tccgacccgc gcaacgtcga atatccggcg gccttggcgg cctccctgat
      421 ggtcgattgg gcggggggcc gagtagctgc tggaaacgtc gtcgtgggtt cggcccccga
      481 acttggtcaa ccgatcgagt tgaggcccga aaggtgtcga gcgatcctcg gtttcgatat
      541 ctcgggtgac gagatggtcg atgccctgac gcgtcttggt ctctcaccga ccctctctga
      601 cggggtcatc cgcgtggctc gccttagtcg ccgaggtgat cttcagttgg aagaggatct
      661 catcgaggag gttgctcgac tcggcggata tgaccgcgtc ccatcgaccc ttgcgtccgg
      721 gcgttaccga gccggatcgc tgacgcgagc acagatactt attcgcagga tccgttcggc
      781 cctcgcgggg gcgggactct tcgaggctta tacgcccaca ttcatcgggc ccgatgatct
      841 cgcagtgctg gggatcgagg atgaccacgc gtggggaggg ccgctccgcg ttaccaaccc
      901 actcagccgc gatgagtcgc tgctgcggac ctcgctcatc cccgggttgc ttcagtgcgt
      961 gaagacgaac atctcgcgcc gcaaccttga catccgactt ttcgagatcg gaagggtctt
     1021 ccacggatcg gatggccaac tcccgaacga acccgaacgc ctcagcgtcg tgatcgcggg
     1081 cacccaacgg caggagtggc tgtcggaaga gcgagagctc gatctcttcg acctcaaggg
     1141 agcgctcgag gtcctcttct catcgctgcg gacacccgac gttcgcttcg tcgattcggc
     1201 agggcacccg gcgcatccga ccagatcctg tcttgttacc accgggggcc ggacgctcgg
     1261 agttctcggt gaactggatc cgacgatcgc cgaagcggcc gatctgggga tcagagtctt
     1321 cttcgccgag atcgaactgg aagcgttgat cgagactgcc gctgatccac aaccgaccca
     1381 gcaggtctgg aggttccccg cggtcctgct cgacctcgcg atggtgatcg aagagagcat
     1441 ccccatcgat gcgatcctcg gggtggtcca cagtgttggg ggtgatcacc tcgaatcagc
     1501 gaggatcttc gatgtgtatc ggggagccca ggtcgaatcg gggtaccgca gcgtggctat
     1561 ctcgctcgta ttccgcaacg aggagcgaac gatgaccgag tccgaggcca tcgaggtccg
     1621 tgatcggatc gcggctgagc tgtcatcgcg cttcggggcg caggtcaggg gaagctgatc
     1681 ctcctagcgc gtttgttaga gcgctcgcga gcgctcgctg ctacttcggc gtgttgggag
     1741 actccacgat ccagaagggc acctgacaca accgctggcc gtccgagaag agttggaaga
     1801 tgaacgcacc ctgggcgggg acagcgaacc ccagcctgaa gtgaagtagg gctccgccgg
     1861 ttcgagtgcg atcgaccccg tgctcaccga acacgatctc gccgttcccc tcgataccga
     1921 taggacgccc gtcagcatcg acgatggtaa aggagacagg cagctgccgg ccgatctcac
     1981 tgcgttctcc gctcaacctg atcacgagtg acatcgggcc tatcgggagg ggataggtgc
     2041 ttcccgccgg gacataccag acgtcggttc caccgcgtac gacatagaag agcccgttct
     2101 gtgcatcggc tgactcggct atgaatgcgt agtcgacctc catgcgagga agtttatcca
     2161 aggaagcggt aggcccgtac tgagagtcgg ttgcatagat atacagaata ctgtatgaat
     2221 atgtcatgag atggaggagt gtctgatggc cgtgcgtacc gctgtcttgg gcgcatcggg
     2281 cttcgccggg tcagaactgc tgcgcctgtt ggaggggcac cctgagactc aggtgatcgc
     2341 cgccactagt tcgtcgcggg tgggggagtc cctagagacg atgtaccccc acttgacgaa
     2401 actggccggg ctccggttcg tgagcatcga ggatgccctg tcccttggag tcgatgtggt
     2461 cttctcctcg cttccgcatg gtgagagcgg gaagctcctt gcccgttacc aaggacgagt
     2521 cgttgatctg gctggcgatt ttcgactgag ggaagcgacc tcctatgaac cgtggtacgg
     2581 cgagcctcac ccgtacccct ccgcgctacc cgagtgggtc tatggcctta gtgagttctc
     2641 tcgcgacaag atcgctggtg cgctcaaggt tgcgaacccg ggttgctacc cgacggccgc
     2701 actcttggca ctcggccccc tggtcgcggg tcagctcgtc gacgcctcat ccatccacat
     2761 cgatgccaaa tcgggcgtct cgggggccgg gcgcgcaggc ggggaagggt tcgacttctc
     2821 tgccatgaat gagaacatgc gcgcctactc tccatcgggt cacaagcaca tcgctgagat
     2881 ggagcaggaa cttgcggtca tggctgggga caccgtgacg atcaccttca tcccgcatct
     2941 cgttccactc actcgcggcc tactcgtcac gtgcatcgca ccggtggccg acggggtgga
     3001 tgcaggaact ctcgatcgcg ccatggctga tgcatatgct tccgaacggt tc
//
LOCUS       DATDOZ010000277         5778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_491437, whole genome shotgun sequence.
ACCESSION   DATDOZ010000277 DATDOZ010000000
VERSION     DATDOZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491437"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VI541_05575"
     CDS             <1..254
                     /locus_tag="VI541_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019524344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF70409.1"
                     /translation="ITQSLITQIEDVRPKPRAAKNLSQRERDVLQLLAFGKSNKEIAR
                     ELGIGSQTVKTHVSHIFTKLGAADRTGAVATALRKGLVE"
     gene            306..557
                     /locus_tag="VI541_05580"
     CDS             306..557
                     /locus_tag="VI541_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70410.1"
                     /translation="MRSSPGRAILSALSVIFTLSACSTSPAPRELAKVSKPNATVHAT
                     SDAAMRNDVEEADSEMVDIAREMSSAGAGLDGPDESDLP"
     gene            557..1300
                     /locus_tag="VI541_05585"
     CDS             557..1300
                     /locus_tag="VI541_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70411.1"
                     /translation="MIGRSIISLALATSVFLTSEPVEAIDRSPSPYASERADRKSDLS
                     PSADAKIEDLRRICLRAIERRLPVLERLALRIETNRRLDDARRDVLSQQVRDVSSGIM
                     ALRGRLASGSSLESLLAECRSVRSRFRVFVVVEPKVHLVIAGDRSLATAEKLENIHGR
                     LTARVDQAQTEGRDMLEARVILDRMKSALDKAKSEAASAIAIAIALDPADHPNNRDQL
                     RAARTLLRSAFADLKQALGEARAVMQLLH"
     gene            complement(1297..2574)
                     /locus_tag="VI541_05590"
     CDS             complement(1297..2574)
                     /locus_tag="VI541_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF70412.1"
                     /translation="MQPPEDSADQHSYRRLLMVLLGTATFFEGYDSGITAIVLRDLAR
                     EFDINTLDTTRLTGPIVVMGFGAFGAMFVTMLGDRIGRRPLMIITTFAYALFTGLTAT
                     SQSLGAFMTYQFFARAFLIAEFATAITIVAEEFPADRRGRALGTLTALGAFGLPLAAV
                     VHLMSAGTSLGWRTVYIVGLIPLMVVGVLRTKLRETARWQAARASGDHLKRVRFRTVL
                     DGEHRRTLLTVSALYFFAHFALLGASTWWPLFVRAHRGFSASAVTALLLSAFPLGVAG
                     FLAAGRLQDRIGRRPTGTIFLVLGLAFGLATFQVRSLALMFPVLAMAVFFGFGVSPVL
                     GAIVTESFPTDIRATSVAFARSIFGTLGATLGPFTVGILADRQLAARVPGWPVLGNLG
                     ACVSVAILMYIPAMFILRRLPETAGAELEAIAN"
     gene            complement(2602..2877)
                     /locus_tag="VI541_05595"
     CDS             complement(2602..2877)
                     /locus_tag="VI541_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HLF70413.1"
                     /translation="MATGDTQKIPRNDTQELGKTKVARRTRRVLVSIVGVALILLGLF
                     LIPLPGPLTLPLVFAGLTVLSWEYAWAKRALLKVRKKWREFRPKVGR"
     gene            2978..3931
                     /gene="glpX"
                     /locus_tag="VI541_05600"
     CDS             2978..3931
                     /gene="glpX"
                     /locus_tag="VI541_05600"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HLF70414.1"
                     /translation="MELVRVTEAAALAAGRWMGRGDKEGADQAAVDAMRTMISTVSMD
                     GVVVIGEGEKDEAPMLYNGEHVGSGVAPKVDVAVDPIDGTGLTSRGQPGALSVIAIAE
                     SGSMFDPGPCVYMEKMATGPEAAGAIDMSVSIEENLHRVAEAKGADVEDITVIILDRD
                     RHAGIIKEVREAGARIRLISDGDVAAAISAASADTGVDLLIGIGGTPEGVIAAAAIKC
                     LGGTIHGRLWPRNEDERSRALAEGYDFDRILTTNDLVAGDCFFAATGITDGELLRGVR
                     YRGSGASTQSIVMRSHSGTIRTITSEHPMNKVTRFAGGVNG"
     gene            3942..4814
                     /locus_tag="VI541_05605"
     CDS             3942..4814
                     /locus_tag="VI541_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HLF70415.1"
                     /translation="MVDSSSLIYRAFYSTSEMRSPDGTPINAAHGFLNMLARLVTDRH
                     PTYMACATDRNWRPQWRVDLIESYKTHRVGTPESTIVDDQMPIIFEILGRLGVAVVGV
                     DDQEAEDVIGALVTMSRGRVEIVSGDRDLFQLVRDPDVVVLYPKRGVSELLVVDEAYI
                     ETKYGIPGKKYGDFALLRGDPSDGLPGVVGIGEKTASSLISKYGSIEGVIGAALVDTT
                     SSALLKVRRALDYLDRAANVVLISGDLAVGDVDMTLPREIDEAALESARGYGLEGALR
                     RLVRALTGERSPEG"
     gene            4819..>5778
                     /locus_tag="VI541_05610"
     CDS             4819..>5778
                     /locus_tag="VI541_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLF70416.1"
                     /translation="MGSIKITTKIPGPRSLELARRGLAAIASPLVPGGEVFAADGLGA
                     VIEDVDGNRYLDFIGGVGCVVTGHNHPRVVDAIHGQVDAFIHTDFSILPYETYVSLAE
                     RLSAASGGDRKCGFFNSGAEAVENAVKIARGVTGRPGIICFEGAFHGRTLMGMSLTHR
                     EVPYKTGFGPFAPEIYRAPYAGFNGSTLDQAIPRIKGLFAEHSIAAVIIEPILGEGGF
                     VPAEEGFLEALSRICAESGAALIVDEIQTGYGRTGSFIASDLVGVRPDIIILGKSIAS
                     GLPLSAVVGSAEWMDVLERNTLGGTYVGNPVACAAALAVLDVIE"
BASE COUNT         1180 a         1664 c         1843 g         1091 t
ORIGIN      
        1 agatcactca gagcctcatc acccagatcg aggacgttcg cccgaaacca agggcggcca
       61 agaacctctc gcaacgcgaa agagatgtct tgcagctact cgcgttcggc aagtcgaata
      121 aggagatcgc tagggaactg ggtatcgggt ctcagaccgt gaagacccat gtctcgcaca
      181 tcttcacgaa gctcggggct gccgatcgca ccggggcagt ggcaacggcc ctgcgtaagg
      241 gactggtgga gtgaggttct ggcggtcttc acgccgtctt caaggtcgag ccacataatc
      301 aagagatgag gtcttcaccc ggtcgagcga tcctatctgc gctttcggtc atctttacgc
      361 tgagtgcgtg ttccacgagt ccagcccccc gggagcttgc gaaggtgtcg aagccgaacg
      421 cgacggtcca cgcgacgagc gacgccgcca tgcggaacga tgttgaggaa gcagactccg
      481 agatggtcga catcgcaagg gagatgtcgt cagcgggcgc tgggctcgac ggccccgatg
      541 agagtgatct cccatgatga tcggacgctc catcatctcg ctcgctctgg ctacatcggt
      601 cttcctgacg tcggagcccg ttgaggcgat cgatcgctct ccgagtccat acgcgtcgga
      661 gcgcgctgat cgtaagtcgg atctttcgcc ctccgccgat gcaaagatcg aggacctgag
      721 aaggatctgt ctgcgtgcca tcgagagacg tttacccgtt ctcgagaggc tcgcactgcg
      781 catcgagact aaccggagac tcgacgacgc gcggcgtgac gtcctgtcac aacaggttcg
      841 cgatgtgtct tcgggcatca tggctcttcg cgggcgcctc gcatctggga gcagcctcga
      901 gtctctgctt gcggagtgtc gaagcgtcag gtctcggttc cgcgtctttg tcgtcgtcga
      961 gcccaaggtt cacctagtca tcgcaggtga tcgatcccta gccactgctg agaaactaga
     1021 gaacatccac gggcgcctca ccgcgcgggt cgatcaggct cagacggaag ggcgcgacat
     1081 gctggaggct cgggtgatcc tcgaccggat gaaatcagcc ctagataagg cgaagagtga
     1141 agcagcatcc gccatcgcaa ttgcgatcgc gctggatccg gccgatcacc cgaacaatcg
     1201 cgatcaactc cgtgcggccc gtaccttgct caggtccgca tttgctgatc tgaagcaagc
     1261 actgggcgag gcgcgggcag tgatgcagct cctccactag ttcgcgatag cctccagctc
     1321 ggcccccgcg gtctctggca atcttcgcag gatgaacatg gccggtatgt acatcagtat
     1381 cgccactgac acgcacgcgc cgaggttgcc cagcacgggc cacccaggca ctcttgccgc
     1441 caattgacgg tctgcgagga tccccaccgt gaagggcccg agcgttgctc caagtgtgcc
     1501 gaagatggat ctcgcgaacg ccacggaggt ggcgcggatg tccgttggga acgattcggt
     1561 gacgatcgcc cccagcacgg gcgatacgcc gaatccgaag aagaccgcca tggcgaggac
     1621 ggggaacatg agggcgagac tacggacctg gaaggtagcg agcccaaagg ccagccccag
     1681 gacgagaaag atggttcccg ttgggcgacg acctatccgg tcctgaagtc tgcctgccgc
     1741 gagaaagccc gccactccga gcggaaaggc gctcagcagc agagctgtta cggctgaggc
     1801 gctgaaaccg cgatgggctc tgacgaagag cggccaccaa gtcgacgctc ccagcagtgc
     1861 gaagtgtgcg aagaagtaga gagcactgac cgtcaggaga gttcgccgat gctctccgtc
     1921 gagtaccgta cgaaaccgga cccgtttgag gtgatcgccc gaagcgcgag ctgcttgcca
     1981 ccgggctgtc tccctgagct tcgtcctcag gactccgacg accatcagcg ggatgagacc
     2041 cactatgtag acggttctcc agcccaatga agtacccgca gacatcagat gtacgaccgc
     2101 agccagcgga agtccgaaag caccgagggc ggtaagtgtt ccgagtgctc gcccgcgccg
     2161 atccgcaggg aactcctcag ctacgatcgt gatcgcggtc gcgaactcag cgatgaggaa
     2221 ggcccttgcg aagaactggt aagtcatgaa ggctcccaga ctttgagacg tcgcagtgag
     2281 accagtgaag agcgcgtagg cgaacgttgt gatgatcatc aaggggcgtc gtccgatgcg
     2341 atcaccgagc atggtcacga acatcgcacc aaaagcaccg aagcccatga ctacgatcgg
     2401 gcccgtgagc cgagtcgtat ccagggtgtt gatatcaaac tctcgtgcca ggtcacgcag
     2461 cacgattgcg gttatgccgc tgtcataccc ctcgaagaag gttgccgttc cgagcagcac
     2521 catgagaagg cgtcggtaag agtgctggtc ggcagagtcc tcgggcggtt gcattgccgg
     2581 gatactctca gagtatgtgg gttatctgcc gaccttgggg cgaaactctc tccacttctt
     2641 ccgcaccttc agtaaggcgc gcttcgccca cgcgtactcc cacgacagga ccgtcaagcc
     2701 agcgaagacc aagggcagcg tcagtggacc cggtaggggt atgaggaaga gccccagcag
     2761 gatcagggcg actccaacta tcgatacgag aacccttcgg gttctgcggg cgaccttggt
     2821 cttccccagt tcctgggtat cgttccgcgg gatcttctga gtgtcacccg tagccatacg
     2881 tcaaggatgc caccttatcg tggtgaggtg gggacgccgg cgaggatagg atggtgacgt
     2941 ggatcagatc acggcaggca atgatcgcaa cctcgggatg gaactcgtgc gtgtcacgga
     3001 ggccgccgcg ctggctgccg gtaggtggat gggccgcggc gacaaagagg gtgccgatca
     3061 ggccgctgta gacgcgatgc gaacgatgat ctcgaccgtt tcgatggacg gtgtggtcgt
     3121 gatcggggag ggcgagaagg acgaggcccc catgctctac aacggcgaac acgtcgggag
     3181 cggggtggct ccgaaagtcg atgttgcagt cgatccgatc gacgggacgg gcctcacctc
     3241 gcggggccag ccaggagctc tctcggtgat cgctatcgca gaatcgggct cgatgttcga
     3301 tccgggcccc tgcgtctata tggagaagat ggctacgggt cccgaagcgg ctggcgccat
     3361 cgatatgtcg gtgagcatcg aagagaatct gcacagggtt gccgaggcca agggcgccga
     3421 cgtcgaggac atcacagtca tcatcctcga ccgtgatcgg catgctggga tcatcaagga
     3481 ggttcgcgag gcgggggccc ggatcagatt gatcagtgac ggcgacgtcg cggcagccat
     3541 ctcggcggcc agcgccgata ccggagtgga tctgctgatc ggcatcggtg ggactcccga
     3601 aggggtcatc gcagcggcgg ctatcaaatg tctggggggt accatccacg gcaggctctg
     3661 gccacgtaac gaggacgaga ggtctagagc gctggctgag ggctatgact ttgatcggat
     3721 cctgactacc aacgatctag ttgcaggtga ctgtttcttc gccgctacgg ggatcaccga
     3781 tggtgaactg cttcgaggcg ttcgctacag agggtcaggc gcctcgaccc aatcgatcgt
     3841 gatgaggtcg cacagcggca ccatccgaac gatcacatcc gaacacccga tgaacaaggt
     3901 tactcggttc gctgggggcg taaacggctg agtcaacgct cctggttgat tcatcgagcc
     3961 tgatatatcg ggctttctac tccacttctg agatgcgctc gccggacggt acccccatca
     4021 acgccgccca cggattcctc aacatgctgg ctcgactcgt gaccgaccgc catccgacct
     4081 acatggcctg cgcgacggac cgcaactgga ggccccaatg gcgagtcgat ctgatcgagt
     4141 catataagac gcaccgtgtt ggaacacccg agtcgacgat cgtagatgac cagatgccga
     4201 tcatcttcga gatcctcggc cggttggggg tagcggttgt cggcgtcgat gatcaggagg
     4261 ccgaggatgt catcggagcc ctggtcacga tgtccagggg ccgagttgag atcgtctctg
     4321 gggaccgaga tctgtttcag ttggtgcgtg accccgatgt ggtcgtgctc tatccgaagc
     4381 gtggagtgag tgagctgtta gtggtcgacg aggcatacat cgaaaccaag tatgggatcc
     4441 ctggtaagaa gtatggagac ttcgcgctgc ttcgaggtga tccttccgac gggctccctg
     4501 gtgtggttgg gatcggtgag aagacggcgt cttcgctcat atcgaagtac ggaagtatcg
     4561 aaggagtcat cggtgccgca ctggtcgata caacctcgtc cgcgctcctg aaggtgcgtc
     4621 gtgcactcga ctacctcgac cgagcagcga acgtggttct gatctcggga gacctcgcgg
     4681 tcggagacgt cgatatgacc ctgccgagag agatcgatga ggctgctctc gaatcggcca
     4741 gaggatatgg tttggagggc gcactgcgcc gactcgtcag ggcgctgacg ggcgagagat
     4801 caccagaggg ttgagcagat gggttcgata aagatcacaa cgaagatacc gggcccccgc
     4861 tcgttggagc tggcgcgcag aggtctggca gcgatcgcat cgcccctcgt ccccggtgga
     4921 gaggttttcg ctgcagatgg tctcggcgcg gtcatcgaag atgtagacgg caaccgttac
     4981 ctcgacttca tcgggggggt cggatgtgtc gttactgggc acaatcaccc gcgtgtggtc
     5041 gacgccatcc acggtcaggt cgatgcattc atccacacgg atttctcgat actgccttac
     5101 gaaacatacg tgagcctcgc cgagagactc tcggccgcat ctggcggtga tcgcaagtgt
     5161 ggtttcttca actcgggggc cgaggcggtt gagaacgccg tcaagatcgc gcgaggcgtg
     5221 acaggtcgcc cagggatcat ctgcttcgag ggggccttcc acggaagaac cctcatgggg
     5281 atgtcgctta ctcatcgaga ggtcccatac aaaactggat tcggaccctt cgcacccgag
     5341 atctatcggg ccccatacgc agggttcaac ggttcgactc tggatcaagc gatcccacgc
     5401 atcaaaggtc tcttcgcaga gcactccatc gcagcagtga tcatcgaacc gatcctcgga
     5461 gagggtggct tcgtccccgc ggaggaagga ttccttgagg cgctctctcg gatatgcgcg
     5521 gagagtggag cggccctgat cgtcgatgag atccagaccg ggtacggacg gacggggtcg
     5581 ttcatcgcat ctgacctcgt tggagtgcgg cccgacatca tcatcctcgg aaagtcgatc
     5641 gcttcgggcc tgcctctctc tgctgtcgtc ggctcagccg agtggatgga tgtcctcgaa
     5701 cgcaacaccc tcggcggaac atatgttggg aatccggtgg cctgtgcggc ggcgctcgca
     5761 gtcttggatg tgatcgag
//
LOCUS       DATDOZ010000278         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1291380, whole genome shotgun sequence.
ACCESSION   DATDOZ010000278 DATDOZ010000000
VERSION     DATDOZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291380"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VI541_05615"
     CDS             complement(<1..405)
                     /locus_tag="VI541_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HLF70417.1"
                     /translation="MSTRTFKIGTRGSHLAMAQATWIASGIRRISSTVRSSIEVITTS
                     GDRAAGAGHLGDGIFVREIQEALLRGDIDVAVHSMKDLPTQEVEGVRLAAVPDRVDPR
                     DAVIGGTLAGLAPGSRVGTSSPRRVAQMLHLRP"
     gene            complement(408..1604)
                     /gene="hemA"
                     /locus_tag="VI541_05620"
     CDS             complement(408..1604)
                     /gene="hemA"
                     /locus_tag="VI541_05620"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HLF70418.1"
                     /translation="MIVALSAGRDSPPRLRARLAMDEAAQSQLLSGPRPGVGELVILS
                     TCHRTELYATADGFEAEVVHSVAALLPELLPTDQHDVRFIQGSEAIEHLFRVACGLDS
                     LVIGEPQVLGQVRRAYNLASEAGAAGPVMHNIFARAIRLGKQVRSETPLGRLGQSIGS
                     IAADYLDLRLRPAAARSGVVVGAGEAATDAAKVLWKSGAELAIVSRTIDSAQTLASLV
                     GGTAHTLEELSDVMIDRDFAVVAILGGAQVTSQDLPRRTPDDPFIILDLSVPQAVVVH
                     DRDDVELRSLEEIPGPRGPEITDAVIEAEAMVKREVAELQKWADTRESGPAIQDLRSH
                     AEALVRGELENGLSGLQLTVEQRERVEILTRRIVNKLLHGPTTAMRRSDESTRTSIKK
                     LFGLDG"
     gene            complement(1620..2540)
                     /gene="hemH"
                     /locus_tag="VI541_05625"
     CDS             complement(1620..2540)
                     /gene="hemH"
                     /locus_tag="VI541_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HLF70419.1"
                     /translation="MTIAVLTMAYGSPNSMDEVEAYFTDIRGGRRPTPEALEGLIARY
                     ARIGGPSTLSQITRLQASSLEDRLNTLAPGRFEVFVGMKHWHPYVSEAVEAIAAKGIK
                     DLIGLVLAPHYSTRSVGQYRDRIDSAMTNLDAQLRLEMISSWFESPKFIDFSAANLMT
                     TLNGWPTHDPGTRVFFTAHSLPESILADGDPYKDQLQASAKLIADAAGIGDWEVAWQS
                     ASPTHEPWIGPDILDSLAAFKDQGGRRALVAPIGFVADHLEILYDVDVECVERAEQLG
                     IEFRRTPSPNTDPRFIDALAEVVFAASRHA"
BASE COUNT          450 a          780 c          820 g          537 t
ORIGIN      
        1 agggcggaga tggagcatct gggcgacacg acgtggactg ctcgtgccca cccgcgatcc
       61 tggggcgagt cccgccaatg tcccaccgat gacagcgtcg cgggggtcga cgcgatcggg
      121 aacagccgcc aatcggaccc cttcgacctc ctgcgtcggg aggtccttca tcgagtgtac
      181 ggcaacgtcg atgtcgcctc tcagcagggc ctcctggatc tctctcacga agattccgtc
      241 gccgagatga cctgctcctg ctgcgcgatc gccgctcgtg gtgatcacct cgatggacga
      301 tctaaccgtc gagctgatcc tcctgatgcc cgaggcgatc caagtggcct gggccatagc
      361 aaggtggctg cctctcgtgc ctatcttgaa ggtgcgtgtg ctcactttca gccgtctagg
      421 ccgaatagtt tcttgatgga ggtgcgagtc gactcatcgg accgtctcat cgctgtcgtc
      481 ggtccatgga ggagcttgtt cactatccgg cgggtcagga tctcgacgcg ctcgcgctgc
      541 tcgaccgtga gctgcagacc cgatagcccg ttctccagct caccccgcac gagagcttca
      601 gcatggcttc gaagatcttg gatggccggg cccgattcgc gcgtatcggc ccacttctgc
      661 aactcggcca cctcacgctt gaccatggcc tcggcctcga tgaccgcgtc ggtgatctcg
      721 ggcccccggg ggcctgggat ctcttcgagg cttcgcagtt cgacatcgtc gcggtcgtgt
      781 accacgactg cttgtggcac ggagaggtcc aggatgatga atgggtcatc cggcgtgcgc
      841 cgaggcaggt cctgcgacgt gacctgtgca ccacccagga tggccaccac ggcgaagtcg
      901 cgatcgatca tcacgtcaga gagttcttcg agcgtgtgag cggttcctcc gacaagtgaa
      961 gcgagcgttt gagcggagtc tatggtcctc gagacgatgg cgagctcggc tccgctcttc
     1021 cagagcactt tggcggcatc ggtggccgcc tcgccggctc caaccaccac gcccgatcta
     1081 gccgctgcag gtcgtaaccg aaggtctaga tagtcggcgg cgatgctgcc gatcgattga
     1141 ccgagtcgcc cgagcggcgt ctcgcttcgc acctgtttgc ccagacggat cgccctggcg
     1201 aagatgttgt gcatgaccgg gcccgcggcc cctgcttcgg atgcgaggtt gtaggccctt
     1261 ctaacttggc caagtacttg aggttcgccg atcaccagag agtcgaggcc gcaggccact
     1321 ctgaagaggt gctcgatcgc ctcgcttccc tgtatgaagc gaacgtcatg ttgatccgtc
     1381 ggaaggagtt cgggaagaag agccgccacg gagtggacca cctcagcctc gaacccatca
     1441 gcggttgcat agagttctgt gcgatggcag gtcgacagga tcaccaactc ccctacaccg
     1501 ggacgggggc ccgataggag ttggctttgg gcagcctcat ccatcgcgag gcgtgccctg
     1561 agccgtgggg gagagtcgcg tcccgcgctg agtgcgacga tcattcgggg gtcagagtat
     1621 taggcatggc gcgacgctgc aaacaccact tcggcgagag cgtcgatgaa cctgggatct
     1681 gtgttcggcg agggcgtgcg gcggaactcg ataccgagtt gctcggctcg ctccacgcac
     1741 tctacgtcga cgtcgtagag gatctcgaga tggtcagcca cgaacccgat gggtgcgaca
     1801 agtgcccgcc ttcccccctg gtctttgaac gccgcaaggc tatcgaggat gtcagggccg
     1861 atccagggtt cgtgggtagg gctcgcgctc tgccaggcaa cctcccagtc tccgatgccc
     1921 gcagcgtcgg cgatgagctt cgctgatgcc tggagttgat ccttgtaggg gtctccgtcg
     1981 gcgaggatgc tctcgggcag gctatgggcg gtgaagaaga ccctcgtccc tgggtcgtga
     2041 gtgggccatc cgttaagagt tgtcatgaga ttggccgccg agaagtctat gaacttcggt
     2101 gattcgaacc atgatgagat catctcgaga cgtaactgtg cgtcgaggtt ggtcatcgcg
     2161 gagtcgatcc tgtctcggta ctgaccgacg ctgcgtgtcg agtagtgcgg tgccagaacc
     2221 agcccgatca gatctttgat gcccttggcc gcgatcgctt cgacggcttc actaacgtag
     2281 gggtgccaat gtttcatgcc tacgaagact tcgaagcgtc ctggggcaag ggtgtttaac
     2341 cgatcctcga gggatgatgc ctgcagcctc gttatctggc tcagtgttga ggggcccccg
     2401 atgcgtgcgt agcgggcgat gagtccctcg agtgcttcag gagtgggcct gcgcccaccc
     2461 ctgatatccg tgaagtaggc ctctacctca tccatggagt tcggacttcc gtaggccatg
     2521 gtcagcaccg cgatggtcat cgcatcgtct cctcatggac cagatcgacc aagaacttga
     2581 tgttgtc
//
LOCUS       DATDOZ010000279         5176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_752416, whole genome shotgun sequence.
ACCESSION   DATDOZ010000279 DATDOZ010000000
VERSION     DATDOZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752416"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VI541_05630"
     CDS             <1..950
                     /locus_tag="VI541_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLF70420.1"
                     /translation="LPVNVRILVEGEEEIGGESVVEWVRDDSEGVDCAVVFDAGMLNE
                     TTPSITLGVRGHIGFDISIRTAESNLHSGLYGGSVVNAAHVMHRILAEVMPGPDGRLR
                     EELRVGIVPPTQAELDSWAMLPPGDDVLAEVGAMPLTPRSGAEYYVRNWADASLDIAG
                     VSGGDAFQIRTIVPSTATATFNMRLAPGQRTAEILPVLKRLVMDAVPPGVEITDIKTR
                     RGEAALFSATEPAIVLAAEALHEATGVKPAFVRSGGSIPILAAFADRGIPTILSGFGL
                     PQDRIHAPDESFRVKSLELAERSARALYSSLSRLEVGTR"
     gene            complement(1115..2392)
                     /locus_tag="VI541_05635"
     CDS             complement(1115..2392)
                     /locus_tag="VI541_05635"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF70421.1"
                     /translation="MTPLLRLTTRTFQSLRVRNYRWYFFAQIISMSGTWMQSVAQGWL
                     VLKITDSGVALGVTAALQFLPVLLFGAWGGVIADRFDKRKTLVATQVAFAILAVALGG
                     LESAGIVRLWMVYVLSLLFGFVSVIDIPVRQSFVVEMVGPQDLSNAVGLNSAIFTSAR
                     VIGPAIAGILISTVGIAICFYVNGASYAAVIVGLMAMDATKLSKRPRVSRAPGQVREG
                     FRYIWSNPKLRHTLMMLAIIGTFSFNFSVLLPLLAKFVFHGGAGLLATMTSVMGAGAL
                     IGALTTAARNRPTRKMLHGSAAIMGVALLLAGIAPTLGLELVLLAVMGGASIAFVSTA
                     NSTLQLTSEDAMRGRVMAMYSVVIVGSSPLGGPLLGWIAEHLGPRAGLQAGGIAALVA
                     VLLSWILVARSRREPQHARAFSDQVSAEPAVLP"
     gene            complement(2389..2850)
                     /locus_tag="VI541_05640"
     CDS             complement(2389..2850)
                     /locus_tag="VI541_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLF70422.1"
                     /translation="MSLTIEESQMNLVTSNPTTVPRLRLVLMRLARKLRQQAAGNVTP
                     SMLSALSSIDSIGPLTLGELATVEQIQPPTMTQIVSRLEDTDYVVRQVDPSDRRVARV
                     QVTPAGKKFLTKNRSRKDAYLAQGLRKLSPSEVETLERAIPILERLLEEQD"
     gene            2923..3924
                     /locus_tag="VI541_05645"
     CDS             2923..3924
                     /locus_tag="VI541_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLF70423.1"
                     /translation="MRYRKILVGTDGSATAAVAVGVAESLAQAHKSHLLLATAVAKQG
                     QSAEVVLSKAQSNLAGLTLRVKTCFGVGPPAEVMLSLATREGADLVVVGDKGMTGPRS
                     LLGSVPDSLSHAGLCDLLIVRTRKAERPGYRLVLIATDGSATSMEAAERGLEVAARSG
                     AQAHLFYAGHPKTAEIVFGEVFAGLGRDGVHHFSKQGDPADVLCEVAQSGGYDLIVLG
                     NKGMGAGRFKLGAVPNKVSHRCATDLLIVKTTGASLSDIGPGQGAVISMDGEKVAAYH
                     VEGDEYILMSPRCSHLGCSVDWNREERTWDCPCHGSRYAPDGSVIDGPAPRPLERKG"
     gene            3921..4397
                     /locus_tag="VI541_05650"
     CDS             3921..4397
                     /locus_tag="VI541_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70424.1"
                     /translation="MTLVQTLLKAIHIFAGLIFMLIHGSSVMMTFRVRRERDRETLKT
                     LLGFSSGTITPMYWTLGALIASGVASGFVGHYWSSWWIWAALTVLLALVGGMVPMAAK
                     YFRRIGQAVGTRPSGAPMASDEELDELLGGSRPMLLAVMGFGGLAVIVWLMVFKPF"
     gene            4477..>5176
                     /locus_tag="VI541_05655"
     CDS             4477..>5176
                     /locus_tag="VI541_05655"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLF70425.1"
                     /translation="MAQATALAREETRPDQVGNTFIPSLKVHRLERRMTLAITLLPFL
                     GLAIGIPILWGRGVGRLDAALLIGFYLVTGLGVTAGFHRMFTHRSFDGPEWLRVAFAV
                     AGSMAIQGPVIRWVADHRRHHAFTDRPGDPHSPHLEEAEGWRGVLRGLWHSHIGWFFD
                     REATNIPRYAPDLLKEKPMVWVDRTFPLWVVVSFLAPAAIGALVTGTLAGALSAFIWG
                     GLLRIFLLHHVTWSI"
BASE COUNT          993 a         1500 c         1663 g         1020 t
ORIGIN      
        1 agctgccggt gaatgttcgc atcctcgttg aaggcgagga agagatcggt ggagagagcg
       61 tcgttgaatg ggtgagggat gattctgagg gggtcgactg tgccgtcgtg ttcgatgcag
      121 gcatgctcaa cgaaacgacc ccctcgataa ctctcggagt acgtgggcac atcgggttcg
      181 acataagcat caggaccgca gagagcaacc tgcactcggg cctctacggt ggcagtgttg
      241 tcaatgctgc gcatgtcatg catcggatac tcgccgaagt catgccgggc cccgacggaa
      301 gactgcggga agaactgaga gtagggatag ttccaccgac gcaggcggaa ctcgactcct
      361 gggccatgct ccctcccggc gatgatgtcc tggccgaggt gggtgccatg ccgctcacgc
      421 caaggagcgg ggctgagtac tacgttcgga attgggcaga cgcatcgctg gatatcgcgg
      481 gggtctcggg aggcgacgcg tttcagatcc gcacgatcgt tccgtcgaca gccactgcga
      541 cattcaacat gcgactcgca cctggtcaga ggaccgccga gatactgccg gtcctcaagc
      601 gtttggtgat ggacgcggtt ccaccgggag tcgagatcac cgacatcaag actcgtcgcg
      661 gtgaggcggc tctgttcagt gcaaccgagc cagcgatagt tctcgccgcc gaagccttgc
      721 atgaggcgac tggcgtgaag cccgcgttcg ttcgaagtgg aggcagcatc ccgatattgg
      781 cagcgttcgc tgataggggg atcccgacga tccttagcgg tttcgggttg ccgcaggaca
      841 ggatccatgc gccggacgag agcttcaggg tgaaaagttt ggagctagca gagcggtcag
      901 ctcgcgcact ttattcctca ctgtcccgtt tggaagtggg gactaggtaa tacgcgctac
      961 tgataaacag ttcgttatga ccacaagatt gatcgtgggg tttccgatat aagagacgtt
     1021 gatccccccg agccgatcag gctctgaaga cacgaaagac cccggcaccc cccccgccgg
     1081 ggtcttttct ttacggtgac gaacttccgt tagctcaggg cagaacggct ggctcggctg
     1141 agacctgatc ggagaatgct cgagcgtgct gtggctctct gcgagatcgt gcgaccaaga
     1201 tccaactgag caagacggcc accagagccg cgatgccccc ggcctgcaaa cctgcgcggg
     1261 ggccgaggtg ctctgcgatc cagccaagca gcggcccgcc gagcggactg ctgccaacga
     1321 tgaccaccga gtacatggcc atcacgcgcc cgcgcatagc gtcctccgat gtcagctgga
     1381 gtgtcgagtt ggcggtcgag acgaatgcga tgctggcacc acccatgacg gcgagaagaa
     1441 cgagctccag tccgagtgtt ggagctatac cggcgaggag tagagcgacc cccatgatcg
     1501 ccgcagatcc gtgaagcatc tttcgggttg gacgattccg ggcggcagtc gtcaatgcac
     1561 cgatgagggc cccggccccc atgaccgatg tcatcgtggc cagcagtccg gcccccccgt
     1621 gaaagacgaa cttggctagc aggggaagaa gaaccgagaa gttgaaagag aaggtcccta
     1681 tgatcgcgag catcatcagg gtgtggcgca gcttggggtt cgaccagatg tatcggaatc
     1741 cctcgcgaac ttgtccaggg gctcgggaga ctctgggtcg cttactgagt ttcgttgcgt
     1801 ccatggccat cagccccacg atcaccgccg cgtaggaggc cccgttaacg tagaagcaga
     1861 tcgcgatccc aaccgttgag atcaagatcc ccgcgatggc agggccgatg acgcgtgctg
     1921 atgtgaagat ggcactgttg agccccaccg cgttgctgag atcttgcggt cctaccatct
     1981 cgacaacgaa actctgtcga acggggatgt cgatgacact gacgaacccg aacagcagcg
     2041 agagaacgta gaccatccat agacgcacga tgccagcgga ttcgaggccc ccgagcgcga
     2101 ccgccaggat cgcgaaggcg acctgtgttg cgaccagggt cttgcgcttg tcgaacctgt
     2161 cggcgataac gcctccccaa gctccgaaga gcagaaccgg caggaattga agggctgccg
     2221 tcacgccaag tgccacgccg ctgtcggtga tcttgaggac cagccagccc tgcgccaccg
     2281 actgcatcca ggttccgctc atcgatatga tctgagcgaa gaaataccaa cggtagttcc
     2341 tgacccgcag agactggaag gttctcgtgg tcaggcgcag aagtggggtc aatcttgttc
     2401 ctccaggagc ctctcgagga tcgggatcgc cctctcgagc gtttcgacct cactcggcga
     2461 aagctttcgc aggccctgag cgagataggc atccttcctg gagcgattct tggtcagaaa
     2521 cttcttgccc gcgggcgtga cctgcaccct cgccactctt cggtccgagg gatcgacctg
     2581 gcgcacgaca tagtccgtat cctccaaccg cgagacgatc tgagtcatgg tcgggggttg
     2641 tatctgctcg acggtggcga gctcgccgag ggtcaggggg ccgatggagt cgatgctgga
     2701 gagagccgag agcatcgatg gggtcacgtt gccagcggcc tgctgtcgca acttgcgggc
     2761 caaacgcatc agcacgagtc tcagacgcgg aaccgttgtt gggttcgagg taacgaggtt
     2821 catctgggac tcctcgatgg ttagggacac taattagtgt agcaaactat tcgtggacga
     2881 gtcgtccatc caatatcatt ccccgacgaa tcggggaggc ccttgaggta tcgcaagatc
     2941 ttggtgggaa cggacggttc tgcgaccgcg gccgtggccg ttggtgtggc cgagagcttg
     3001 gcccaagctc acaagagcca tctgcttctg gctaccgccg tcgcaaagca gggccagagc
     3061 gctgaagtgg tgcttagcaa ggcccagagc aacttggctg ggctgaccct acgggtaaag
     3121 acatgtttcg gcgtggggcc cccagccgaa gtgatgcttt cgctcgccac gcgggaaggc
     3181 gccgacctgg tcgttgttgg cgacaagggc atgaccggcc cccggtcgct cctcggaagc
     3241 gtgccggact ccctttcgca tgccggtcta tgtgatctgc tcatcgtcag gactcgaaag
     3301 gccgagcggc cgggttatcg gctcgttctc atcgcaacgg atggctccgc cacttcgatg
     3361 gaggcggccg agcgtgggct cgaagtcgcc gcgcgctctg gtgcgcaggc ccacctcttc
     3421 tatgcgggtc atcccaagac cgctgagatc gttttcggcg aggtgttcgc gggcctaggc
     3481 cgtgacgggg tccatcactt ctcgaagcag ggggatccgg cagacgtcct gtgcgaggtc
     3541 gcacagtcgg gtggatacga cctgatagtg ctgggtaaca aagggatggg ggccggtcga
     3601 ttcaagctgg gtgctgttcc gaacaaggtg tcacaccggt gcgcgaccga cctgctgatc
     3661 gttaagacca caggcgcgag tctctcggat atcgggccgg ggcaaggcgc tgtgatctcc
     3721 atggatggcg agaaggtggc cgcgtaccat gtggagggcg acgagtacat cctcatgtca
     3781 ccgaggtgct ctcatctcgg atgttcggtc gactggaatc gcgaggagag gacctgggac
     3841 tgcccatgtc acggctcccg ctatgctccc gatggttccg tgatcgatgg tccggccccc
     3901 cggcccctag aacgcaaagg ttgaccctcg tgcagactct gcttaaggca atccacatct
     3961 tcgccgggct gatcttcatg ctgatacatg gatcttccgt gatgatgaca ttcagggtca
     4021 gacgcgagcg cgaccgcgag actctcaaga ctttgcttgg tttctcgagc ggcacgatca
     4081 caccgatgta ctggaccctt ggtgccctca tcgcgagcgg agtggcctcg ggtttcgtgg
     4141 ggcattactg gtcgagctgg tggatctggg ccgcgctgac cgtgctcctc gcattggtgg
     4201 gcgggatggt acccatggct gcgaagtact tcaggcgcat cgggcaagcc gtggggacca
     4261 ggccctcggg agcacccatg gccagcgatg aggagctcga tgagctcctg gggggttcgc
     4321 gcccgatgct cctggctgtc atggggttcg ggggcctggc ggtgatcgtc tggctgatgg
     4381 tcttcaagcc attctgagcg ggggcttgta acttactggt ccgtaggtta cgctaccgta
     4441 ggttataaag aagccgttta ctggaaagga ccccccatgg ctcaggcaac ggcgctagcg
     4501 cgcgaggaaa caagacccga tcaggttggc aataccttca tcccatccct caaggttcat
     4561 cgccttgagc gaaggatgac cctcgcgata accctcctgc ccttcctcgg cctcgcgatc
     4621 ggtataccga tcctgtgggg aaggggcgtg ggccggctcg atgcagcgct tctgatcggt
     4681 ttctacctgg tgaccggcct cggggttacg gctggctttc accgcatgtt cacccaccgc
     4741 agtttcgatg gaccggagtg gctgcgggtc gctttcgcgg ttgccggatc gatggcgatc
     4801 cagggccccg tcatccgttg ggtggcagat caccggcgtc atcacgcatt caccgaccgc
     4861 cctggtgacc cacactcacc tcacctcgag gaagccgagg ggtggcgtgg cgttctgagg
     4921 gggctgtggc actcgcacat aggctggttc ttcgatcgcg aagccacgaa catcccgcgg
     4981 tatgcaccgg acctcctgaa agagaagccc atggtctggg ttgatcggac attcccgctc
     5041 tgggtggttg tttcgttcct agctcccgca gcgatcggtg cactcgtgac aggaacgcta
     5101 gccggcgctc tttcggcgtt catatgggga gggctactgc ggatcttcct gcttcaccac
     5161 gtgacttgga gtatca
//
LOCUS       DATDOZ010000280         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3620774, whole genome shotgun sequence.
ACCESSION   DATDOZ010000280 DATDOZ010000000
VERSION     DATDOZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3620774"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..610
                     /gene="raiA"
                     /locus_tag="VI541_05660"
     CDS             239..610
                     /gene="raiA"
                     /locus_tag="VI541_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLF70426.1"
                     /translation="MEITVRSKNGSLPRSLETFAIEKLEHLGRFLSTINVIQVEFDQE
                     GRHHADPTHVVRVTVSTPGPVFRSRVTSSDPRAALDAAASRLERRLKEFKRMRSGKPA
                     HSRPKGISADMLGVDENPAED"
     gene            658..1302
                     /locus_tag="VI541_05665"
     CDS             658..1302
                     /locus_tag="VI541_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF70427.1"
                     /translation="MIVDDHSLFRKGLIMVLESEEGIEVVGEAGDGAEALAKAEEAVP
                     DVVLMDVRMPKRSGIEATSAMKDLLPNCKILMLTISDEESDLYEAIKAGASGYLLKEI
                     SIEEVANAVRAVYGGQSLISPSMASKLLSEFATMVKKTDDRESVPVPRLTNRELEVLK
                     LVARGSNNRDIAKELFISENTVKNHVRNILEKLHLHSRMEAVVYAVREKMLDIT"
     gene            complement(1312..1788)
                     /locus_tag="VI541_05670"
     CDS             complement(1312..1788)
                     /locus_tag="VI541_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70428.1"
                     /translation="MRRFPVAVLLAVLLLNTTTASALDRPPITRPYIGGTGDVVALCQ
                     NSIANNLPTSTVSTVGGACFDTLPNDISVKITIADDHHPNASGFVLLEDATGTFIGIP
                     LCNRETTLSLQYVPAVKRVVVQMDESYSILKGVIGSCPGRLPATRGTITAVFHQPN"
     gene            complement(1914..>2370)
                     /locus_tag="VI541_05675"
     CDS             complement(1914..>2370)
                     /locus_tag="VI541_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70429.1"
                     /translation="NHTRVKRFVVALVMSLGVATGMPATASVTTTTETAQYVAGAGDF
                     VVVCANQLTSGQAPGVGGACFDLNGSETTATVTVNDASGRAVGGFWGVDSDNAAGWAF
                     CGSAVIPLSYPGATRLEIYVEEAFSSIDCPAPGAGTKGTISVVLESPDP"
BASE COUNT          489 a          677 c          744 g          460 t
ORIGIN      
        1 agtccatgcc agtcgcaggc gaaaggatcc acgtaaggca accattggtt gttgagcatg
       61 gtgcgactta ggggtcagac ctgccccctc gtcgggagag tcccggcaca ggggagaagg
      121 atcgaagccg gttcagatgg atctcgtcgt gacggctagc cgggcgagat ccgggtcggc
      181 gagacctcag caggcggatg tggggccaaa gaccaggtca gccagaggga ggccatttat
      241 ggagatcacg gttagaagta agaacggctc gctgccgaga tctctcgaga ccttcgccat
      301 cgagaagttg gagcacctcg ggcgattcct ctcgacgatc aacgtgatcc aggttgaatt
      361 cgaccaggag ggcaggcacc acgccgaccc gactcacgtg gtgagggtca cagtatccac
      421 tccaggtccc gtctttcgct ctcgggtgac ctcatccgac cccagagcgg ctctcgatgc
      481 ggcggccagc cgtctggagc gcagactcaa ggagttcaag cgtatgcggt cgggcaaacc
      541 ggcccattca aggcctaagg gtatttctgc cgatatgctt ggtgttgacg agaatcccgc
      601 cgaagactag acaacatcgt cagaggagta tgagaagtga ctgaccctat tagagtcatg
      661 atcgtcgatg accactccct cttccgtaag ggcctcatca tggtgctcga gtccgaagag
      721 ggtatcgagg tcgtcggcga ggcaggggac ggggccgagg ccctggcgaa agccgaagag
      781 gcggttcccg atgtcgtcct gatggatgtg cgcatgccca agcgttcggg tatcgaggcg
      841 acctcagcca tgaaggacct tctgcctaac tgcaagatcc tcatgctcac gatctccgat
      901 gaggagtctg atctctacga agccatcaag gctggagcgt ccggttatct gctgaaggag
      961 atctctatcg aagaggttgc caacgcagtt cgagccgtct acgggggcca gtcacttatc
     1021 tcaccgtcga tggcgtcgaa gctcttgtcc gagtttgcga cgatggtgaa gaagaccgac
     1081 gaccgcgaat cggttcctgt gccgaggctc acgaaccgcg agctcgaagt actcaagctg
     1141 gtcgcgcggg gctccaacaa tcgagacatc gcaaaggaac tcttcatctc tgagaacacc
     1201 gtgaagaacc atgtgcgcaa catcttggag aaactgcacc tgcactcacg catggaggcg
     1261 gttgtctacg ccgtgcgcga gaagatgctc gacatcacct gatagccaag cctagttggg
     1321 ctggtggaag accgccgtga tcgtgcctcg ggtcgcgggc aggcgccctg ggcagctacc
     1381 gatcacgcct tttagtatcg aataggactc gtccatctga acgacgaccc gcttcaccgc
     1441 tggcacatac tgcaggctaa gagtggtttc cctattgcag agcgggatgc cgatgaaggt
     1501 gcccgtggca tcttctagga gaacgaaccc gctagcattt gggtggtggt cgtcggcgat
     1561 agtgatcttg accgagatat cgttgggcag cgtatcgaaa caggctcccc ccacagtgct
     1621 aacggtgctc gtcggcagat tgttggcgat cgagttctgg cagagcgcga cgacatcgcc
     1681 cgttccgccg atataaggcc gagttatggg cggacggtcg agcgccgagg cggtcgtggt
     1741 gtttagaagc aggacggcca atagcaccgc cactggaaac ctacgcatcg ccacctttcc
     1801 gggaggtcat ccccaatgcg gactggtata tttgcactag tcatacgaca gaaaaaccgg
     1861 atttcctgcc tgatctcctt attactctca gaattgtggg cgggcaggct aacttagggg
     1921 tctggcgact ccaggacgac gcttatcgtc cccttggtgc ccgctcctgg ggcggggcag
     1981 tcgatcgagc tgaaggcctc ctcgacgtag atctccaggc gtgtcgcacc cggatacgat
     2041 agcgggatga cggcggagcc gcagaaggcc cagcccgcgg cgttatctga gtcgaccccc
     2101 cagaaccctc cgacggcccg gcccgaggcg tcgttgacgg tgaccgtggc ggtggtttca
     2161 gatccgttta ggtcgaagca tgcaccgccg accccggggg cctggcccga tgtcagctgg
     2221 ttggcgcaga cgaccacgaa gtctccggct cccgccacgt actgcgcggt ttcggttgtg
     2281 gttgtcacgg aggctgtggc cggcatgccg gtcgcgaccc cgagggacat cacgagcgcg
     2341 acaacgaatc gtttcaccct tgtgtggttc
//
LOCUS       DATDOZ010000281         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3622044, whole genome shotgun sequence.
ACCESSION   DATDOZ010000281 DATDOZ010000000
VERSION     DATDOZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3622044"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VI541_05680"
     CDS             complement(<1..1157)
                     /locus_tag="VI541_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HLF70430.1"
                     /translation="MSAPALSSTNGVVSVEIDGVVDPIEASFISSQIRTANVEKANAV
                     VIRIDTPGGLDTSMRKIVGSILESKVPVLCLVSPSGARAASAGTFILLACHVSAMAPG
                     TAVGAAHPVGISGLASREKVVNDSVAFIRSIAQERERDADWAERAVRESVSVSADEAL
                     RLGLIDFKVRSTSELLEATAGMSLRVGGRDVVITSSPRETSQSLGFGPNLLHRALTPD
                     FAFAFFYLGLILLAVELLHPGVSVPGVLGVSLLAMSLLAFGMLPVRLSGLTLLAVSVA
                     FFIVEVKHPGLGLPAVGGVVSLVLGGLLLFDRSVGEGVSLSVIIPVALGVAAFSTLVV
                     KAAMKVRRRPPSMSVEKLIGLVGEARSDLDPRGSIQVAGESWSAKASHGSIP"
     gene            complement(1224..>2179)
                     /locus_tag="VI541_05685"
     CDS             complement(1224..>2179)
                     /locus_tag="VI541_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HLF70431.1"
                     /translation="RGKGDWIPNRLGGRENLEAIEFFRELNTLLHGRVPGIMVIAEDS
                     TAWPGVTLPVDAGGLGFGYKWSLGWMHDTLRYFGREPSHRQHHHEDLTFGLLYQWSEN
                     FILPLSHDEVVHEKGSLLSRMPGDDDERFAGLRALFAWMWAHPGKPLLFMGGEIAQYR
                     EWSHDRELDWHLLSEGPRHKGVQMLIKKLNGIAREHPSLWEQDKSADGFEWIEAADRV
                     NGIYAFLRKSSGPPLACVANLSAIRREGYRVGLPTEGEWVCILDSQDERFGGASSRSG
                     GPIIAEPVPTSTMPCSAPIDLAPLSVLWLIQKPGEALTAVP"
BASE COUNT          479 a          690 c          648 g          362 t
ORIGIN      
        1 ggtatcgatc cgtgagatgc cttcgcgctc cagctctccc cggccacctg gatgcttccg
       61 cgcggatcga gatcgcttcg tgcttcaccg acaagaccga tcaacttctc cactgacatc
      121 gagggtggcc gtcgccgaac cttcatcgcg gcctttacta ccagggtcga gaatgcggcc
      181 accccaagag cgaccgggat gatcaccgag agagaaaccc cttcgccaac ggaacgatcg
      241 aatagcagta atccgccgag taccagactt actacgccgc cgaccgccgg gagccccagg
      301 ccgggatgct tgacctcgac tatgaagaag gcgaccgaaa ccgctagaag ggtcaagccg
      361 ctgaggcgca ccggcagcat cccgaacgcg agcaaggaca tcgccaagag cgaaacaccc
      421 aacaccccag gaaccgatac ccccggatgg agaagctcga cggccagaag tatgagaccg
      481 agatagaaga aagcgaatgc gaaatcgggg gtcaaggccc ggtgcaagag gttgggtccg
      541 aacccgagag attgcgaggt ctcccgcgga gaagatgtga tcacgacgtc ccggcccccg
      601 acccgcagtg acatcccagc cgtagcttcg agaagttcgc tcgttgaacg tactttgaaa
      661 tcgatcagac cgagtcgcaa tgcctcgtcg gccgaaacac tgacggactc acgaaccgcg
      721 cgttcggccc aatcagcatc acgttcccgt tcctgggcga tgcttcggat gaaggccacg
      781 gagtcgttga cgaccttctc gcgcgaagcc agacccgata tcccaacagg gtgagcagca
      841 ccgaccgcag tgcctggggc catcgcactc acatgacatg ccagaaggat gaaggtcccc
      901 gcggatgccg cccgagcccc cgatggagaa accaggcaga ggacggggac cttcgactcg
      961 agtatggaac caacgatctt gcgcatggat gtgtcgaggc cgccaggggt atcgattcgg
     1021 atcaccaccg cgttcgcctt ctccacgttc gccgtgcgga tctgggatga gatgaaggat
     1081 gcctcgatcg gatcgacgac gccatcgatc tccacggata cgaccccgtt cgtggatgaa
     1141 agtgcaggcg cggacatcag accgatgaaa acaaggacca gaccagccag ataccgcaca
     1201 cttgcatcat ctcagatggg gggctagggc actgcggtga gggcttcccc cggcttctgg
     1261 atcagccaga ggaccgagag tggggcaagg tcgatggggg ccgaacaggg catggtcgaa
     1321 gtcggcacgg gctcggcgat gatggggccc cccgatcgcg aagaagctcc cccgaaccgt
     1381 tcgtcttggc tatcgaggat gcagacccac tcaccttcgg tcggcaggcc gactcggtaa
     1441 ccctcacggc gtatcgcaga caggttggca acgcacgcga gcggggggcc cgatgacttg
     1501 cgcaggaacg cgtagatacc gttgacccgg tccgcagctt cgatccactc gaaaccatcc
     1561 gcagacttgt cctgttccca tagggagggg tgttcccgag cgatcccgtt caacttcttg
     1621 atcaacatct ggaccccctt atgcctaggt ccctcggaga gcagatgcca gtcgagttca
     1681 cgatcgtgtg accactcccg gtactgcgcg atctcgcctc ccatgaagag cagaggcttg
     1741 ccaggatgcg cccacatcca tgcgaagagc gcccggagac ccgcgaagcg ctcatcatcg
     1801 tcacccggca tccgacttag cagagagccc ttctcatgta cgacctcgtc atggctcagc
     1861 gggaggatga agttctcgct ccactggtag agcaggccga aggtgaggtc ctcatggtgg
     1921 tgctgacggt ggctcggctc gcggccgaag taacggagcg tgtcatgcat ccagccgaga
     1981 ctccacttgt aaccgaaccc caggcctccg gcatcaaccg gaagggtgac cccaggccat
     2041 gcggtcgaat cctcggcgat gaccatgatg ccgggcaccc gtccatgcag gagcgtgttg
     2101 agttcgcgga agaactctat ggcttcgagg ttctcccttc ctcccagccg attggggatc
     2161 caatctccct tccctcgcg
//
LOCUS       DATDOZ010000282         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2589743, whole genome shotgun sequence.
ACCESSION   DATDOZ010000282 DATDOZ010000000
VERSION     DATDOZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589743"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VI541_05690"
     CDS             <1..645
                     /locus_tag="VI541_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HLF70432.1"
                     /translation="LARICEASGADVVAIADIMGSDPRIGREFLNAGLGYGGYCFPKD
                     VAAFKTQAAKLGYEFGLLDEVVKINRGALDATFAKIKDALWNLEAKKILVLGLAFKGG
                     TDDVRESPALGLAEELIDAGARVVGYDPVAGPAAAERVARLGLTDDIYDGAVEAHCLV
                     IATDWPEFAEIDLVRLKGIMTHPIIVDARNLLSPLAAQEAGFTYIPTGRPPVNL"
     gene            647..1651
                     /locus_tag="VI541_05695"
     CDS             647..1651
                     /locus_tag="VI541_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HLF70433.1"
                     /translation="MRILVTGGAGFLGSHLCERLVMEGHEVVCYDSLLTGDTANMSGL
                     WDNERFTFVEYDVTHFVGIQGHVDWVMHFASPASPLDYLEHPIHTLKVGALGTLNGLG
                     LAKEKGAGFFLASTSEVYGDPLVHPQREDYWGNVNPIGPRGVYDEAKRYAEALVYAYH
                     RVWGLPVRVVRIFNTYGPRMRQADGRAVPNFISQALAGEPITVHGDGSQTRSLCYVSD
                     LIDGFVKMLLSPPHSGPINLGNPREVTVLEIANLIKRISGSQSAIEFVGRPVDDPERR
                     CPDISVAQSVLGWNPGIDLEKGLAWTIDWCREHWSDITEQELGAMRRDRLSEQTDELN
                     "
     gene            complement(1648..>3377)
                     /locus_tag="VI541_05700"
     CDS             complement(1648..>3377)
                     /locus_tag="VI541_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019549674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HLF70434.1"
                     /translation="SMTINATAMWLLALYVTVAEEQGAHRSLLQGTTQNDILKEYLSR
                     GTYIYPPGASMRLTVDMVIWTLHNTPRWNPINVCSYHLQEAGADPVLEIAYSLANAIA
                     VLDATRQTGQVSPDDFERLVGRISFFCNAGMRIVDELCKMRAFTRLWDRITLERYGVQ
                     DPRYRRFRYGVQVNSLGLTASQPENNVYRILLEMLSVTLSKDARARAVQLPAWNEALG
                     LPRRWDQQWSLRAQQILAFETDLLEHEDLFEGSTVVQARTDEIGQAADAELQKVLALG
                     GSLASIDYMKEQLVAAHAGRRRAIESKEQMVVGVNGYEDTEPSGLVDPGASQDESFQT
                     VDEDAEHSAIRNLMEWKSRRDEQQVKQALEALRSAAAADINLMEPSIAAARAGVTTGE
                     WADALREIFGEFRAPTGVTDLSASSTSVSSGIEGARKSVASAAQRLGVGRLRLLIAKP
                     GLDGHSNAAEQLAIRARDAGFEVIYQGIRLSIDQIARAAVEEDVHAIGVSILSGAHNF
                     LVAELVARLGEMGVDIEKVPLIVGGVIPAADIERLQTAGVARVFTPLDYDLGAAVAEI
                     ADLIASRTG"
BASE COUNT          643 a         1035 c         1005 g          694 t
ORIGIN      
        1 ctggctcgca tctgcgaagc atctggagcc gatgtcgttg cgattgccga catcatgggt
       61 tccgaccctc gcataggacg cgagttcctg aacgccggtc ttggctacgg gggctactgt
      121 ttccccaagg acgtggcggc cttcaagact caggccgcga agctcggcta tgagttcggc
      181 ttactcgatg aagtggtcaa gatcaatcgc ggcgcactcg atgcgacatt cgccaagatc
      241 aaagatgcac tttggaatct tgaagccaag aagatccttg tcctcggtct ggctttcaag
      301 ggaggaaccg atgacgtgcg cgagtcaccc gcacttgggc ttgccgaaga gttgatcgac
      361 gctggcgcca gggttgtggg ttatgacccc gtcgcaggac ctgcagcagc ggagcgtgtt
      421 gctcggctgg gtcttacgga cgacatctac gacggagccg tagaggctca ctgtctcgtg
      481 atcgccaccg attggcctga gttcgccgag atcgatctcg tgcgactcaa ggggatcatg
      541 actcacccga taatcgtcga cgctcgcaat ctgctctcac cccttgccgc ccaagaggca
      601 gggttcacat atatcccgac cggccggccc ccggtcaacc tctgacatga ggatcctcgt
      661 aaccggcggt gcgggtttcc tcggctcaca tctatgcgag cggttggtga tggaaggaca
      721 tgaggtcgtc tgttacgact cactgctcac cggcgacacg gcgaacatgt cagggctctg
      781 ggacaacgag cgcttcacat tcgtcgagta cgacgtaact cacttcgttg gcatccaagg
      841 acatgtggat tgggtgatgc acttcgcgtc accggcatct ccgctcgact acctcgaaca
      901 tccgatccat accctcaaag tcggcgcact cggcactctc aatggcctgg gtctcgcgaa
      961 ggagaagggg gccgggttct tcctagcctc cacctcggag gtctacggcg atccattggt
     1021 tcacccacag cgcgaggact actggggcaa cgtgaatccc atcgggcctc gtggcgttta
     1081 tgacgaagcg aagcggtatg ccgaggccct cgtctacgcg tatcaccgcg tgtggggttt
     1141 gcctgtgagg gttgtgagga tcttcaacac ttatgggccg aggatgcggc aggccgatgg
     1201 aagggccgtg ccgaacttca tcagccaggc cctcgcgggt gagcccataa ccgtccatgg
     1261 cgacggctcg cagactcgca gtctctgtta tgtgagcgac ctcatagacg gattcgtgaa
     1321 gatgctgttg tcgccaccgc actcgggtcc gatcaatcta gggaatcctc gcgaggtgac
     1381 ggtcctcgag atcgcaaacc tcatcaagcg gatcagcggt tcgcagtcag ccatagagtt
     1441 cgtgggaagg cccgtcgatg atcctgaacg ccgatgccct gatatctctg ttgcgcagag
     1501 tgttcttgga tggaacccgg ggatcgatct cgagaagggc ctagcgtgga cgatcgattg
     1561 gtgccgtgag cactggtcgg acatcaccga gcaggagctg ggtgcgatgc gccgcgaccg
     1621 gctctcggag cagaccgacg agctgaatta accggttcga gaagcgatca ggtctgcgat
     1681 ctcggcaacc gctgctccaa ggtcgtagtc cagtggtgtg aatacccttg cgacaccggc
     1741 cgtttgcagc ctctctatgt cagccgccgg gatgacaccg cccacgatca aggggacctt
     1801 ctcgatgtca acccccatct cgccaaggcg cgcgaccagt tcagccacga ggaagttgtg
     1861 ggcacccgac aggatggata cgccgatcgc atgaacatcc tcttcgacgg ccgcgcgggc
     1921 gatctggtcg atcgagagcc tgatgccctg gtatatgacc tcgaatcctg catcacgtgc
     1981 gcgtatcgcc aactgctctg ccgcattcga gtgtccgtct agccccggct tggcgatgag
     2041 cagccgcaac ctacccaccc ctagtcgttg cgccgccgac gctacgctct tcctggcacc
     2101 ttcgataccg gatgaaacgg atgtcgaact cgcggagaga tcagtaacgc ccgtgggggc
     2161 cctgaactct ccgaagatct cgcgaagagc atcggcccac tctcctgttg tgacaccagc
     2221 tcgggcggcc gcgatagagg gctccatcag gttgatgtcc gcggccgccg ccgacctcaa
     2281 ggcttcgagc gcctgcttga cctgctgttc gtctcgacgg ctcttccact ccatcagatt
     2341 gcggatcgct gagtgttcgg catcttcgtc aacagtctgg aaagactcat cctgcgacgc
     2401 gccgggatcg accaatcccg agggctcggt gtcctcatag ccgttcacac ccacgaccat
     2461 ctgctccttc gactcgatgg cgcgcctgcg gccggcgtgt gcggcaacga gttgctcctt
     2521 catgtagtcg atgctggcca acgatcctcc gagggcgagg accttctgaa gctcagcatc
     2581 ggccgcctgg ccgatctcgt cggttcttgc ctgcacgacc gtggatccct cgaataggtc
     2641 ttcgtgttcg agcaggtcgg tctcgaacgc gaggatctgc tgagccctca gcgaccactg
     2701 ctggtcccat cgtctcggca ggccgagggc ctcgttccat gcgggaagtt ggacggcgcg
     2761 tgcgcgagcg tccttcgaca gcgtcaccga gagcatctcg agcaggatgc gatagacgtt
     2821 gttctctggc tgactggcgg tcagacctaa cgagttgacc tggaccccgt atctgaaccg
     2881 acggtatctc ggatcctgca cgccatatcg ctcaagcgtg atccgatccc acagacgcgt
     2941 gaacgctcgc atcttgcaga gttcatcgac gatccgcatc cccgcgttgc agaagaatga
     3001 gatccgtccc accaatctct cgaaatcatc aggagaaacc tgacccgtct gcctggtcgc
     3061 atcgagaacg gcgatggcgt tagccagcga gtatgcgatc tcgagcacgg ggtcggcccc
     3121 cgcctcttga aggtgatacg agcagacgtt gatcgggttc catcgtggcg tgttgtggag
     3181 tgtccagatg accatgtcga cggtcagcct catcgaggcc cccggcggat agatgtaggt
     3241 ccctcgcgac aggtactcct tcaagatgtc gttctgggtc gtgccctgaa gcagtgaacg
     3301 gtgcgccccc tgctcttcgg caaccgtcac atagagcgcg agcagccaca tcgctgtcgc
     3361 attgatcgtc atcgaag
//
LOCUS       DATDOZ010000283         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1813053, whole genome shotgun sequence.
ACCESSION   DATDOZ010000283 DATDOZ010000000
VERSION     DATDOZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813053"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VI541_05705"
     CDS             <1..635
                     /locus_tag="VI541_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HLF70435.1"
                     /translation="VDMTAILRLREKAGDAFKEREGFSLTWMPFLCKAVVTALQRFAQ
                     VNATWNGDGTITRRHYVNLGIAVALENGLIVPVVKGADGMNVPGLARGVRDVADRARN
                     KKLSPDEVHGGTFTITNPGPFGSILSVPIMNKGQAAILAFDAVVKRPVVVTDEMGNDS
                     IAIRHMVFISMSWDHRIIDGAEAAQFLAHLKALLEEADFSSDLSGYLTEA"
     gene            636..1394
                     /gene="lipB"
                     /locus_tag="VI541_05710"
     CDS             636..1394
                     /gene="lipB"
                     /locus_tag="VI541_05710"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HLF70436.1"
                     /translation="MKALGVVDAGTVPYRTALAWQLDLHARRLADEIADVVLLLEHPH
                     VYTLGRRFAKDHLLAGEDTLREQGIDVVETDRGGSVTYHGPGQLVAYPILDLRRPGQQ
                     YPDAIRYLRQLEEAIIRTVRGLDVAAGRREGLTGVWVGNAKLASIGVNVSRGITRHGF
                     ALNVSTDLRKFEMMVPCGIPECEMTSLEVLLRRPISVAEVFDRTSRHLAKVLHRRATP
                     MTLAELGLPATSPDAGDARMLDAKIIDFPGAAEV"
     gene            1399..>2050
                     /locus_tag="VI541_05715"
     CDS             1399..>2050
                     /locus_tag="VI541_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLF70437.1"
                     /translation="MAKVEERAVRQVRTAGGRSVTFEEVEADYRLAFASRVASLIGRK
                     EVLTGKAKFGIFGDGKEVAQLAMAKAFADGDWRSGYYRDQTFMLAVGLLTFREFFGQL
                     YANTDRSEDPGSMGRQMGSHFATRFIDDDGAFIPQVSAKHSAADLSPTAGQMARLLGL
                     AYASKLYRNNPALAGSTDFTNGGNEIAFGTIGNAATSEGIFFETMNAAGVLQVPILM"
BASE COUNT          400 a          633 c          636 g          381 t
ORIGIN      
        1 aggtagacat gaccgccatc ttgcgactgc gcgagaaggc cggagatgca ttcaaagagc
       61 gtgaggggtt ctcactgacc tggatgccct tcctctgcaa ggccgtcgtc accgcgcttc
      121 agagatttgc acaggtcaac gccacctgga atggcgatgg aaccatcacg cgccgacact
      181 acgtgaacct cgggatcgcg gtggcactcg agaacgggtt gatcgttccg gttgttaagg
      241 gggccgacgg catgaacgtc ccagggctgg cgcgcggagt tcgcgatgtt gccgaccgtg
      301 cccgcaacaa gaagctgagc cccgatgaag tgcatggcgg cacattcacc atcaccaacc
      361 ccggcccatt cggctccatc ctctcggttc cgatcatgaa caagggccag gcggcgatcc
      421 tcgccttcga cgcggttgtg aaacgcccgg tcgtcgtcac cgatgagatg ggcaacgact
      481 cgatcgccat ccgccacatg gtcttcatct cgatgtcgtg ggaccatcgg atcatcgatg
      541 gcgccgaggc ggctcagttc ctcgcgcatc tgaaggcact tctcgaagag gcagacttca
      601 gttcggacct cagcgggtac ctgacggagg catagatgaa ggccctcggg gtcgtcgatg
      661 cgggaacggt cccctacagg actgctctag catggcaact cgaccttcat gcgcggcggc
      721 tcgccgacga gatcgccgat gttgtcctgc tgctggagca ccctcacgtc tacacgcttg
      781 ggcggaggtt tgcgaaggat cacctcctcg ccggtgagga caccctgcgg gagcaaggta
      841 tcgacgtggt cgagaccgat cgtggtggga gtgtcaccta ccacgggccc ggacagctgg
      901 tcgcttaccc gatactcgac ctgcgccgcc ccggtcagca atatccagat gcgatccgat
      961 atctccgcca gctcgaagaa gccatcatca gaaccgtccg cggtctcgat gtcgcggccg
     1021 ggcgccgcga ggggctcacc ggggtctggg tcggcaacgc caagctcgct tccatcggtg
     1081 tcaacgtcag taggggcatc acgaggcacg gcttcgctct caatgtgtcg accgatcttc
     1141 gcaagttcga gatgatggtc ccctgcggga tccccgaatg cgagatgacc tcgctcgaag
     1201 ttctgttgcg tcggcctatc agtgtcgcag aggtgttcga tcgaacgagc cgacatctgg
     1261 ccaaggtatt gcatcgcagg gcgacaccga tgaccctcgc agagctcggc ctcccggcga
     1321 catcgcccga tgcgggcgat gctaggatgc tggacgcgaa gatcatcgat ttcccgggtg
     1381 cagcagaggt ctagtccaat ggcaaaggtc gaagaacgcg ccgtccgtca ggtccgaacc
     1441 gcaggcggcc gaagcgtcac attcgaggaa gtcgaagccg actatcggtt ggctttcgcc
     1501 agccgcgtgg ccagtctcat cggtcgcaag gaggtcctga ccggcaaggc gaagttcggg
     1561 atcttcggag acggcaaaga ggtggcacaa ctcgcgatgg ccaaggcctt cgccgacggc
     1621 gactggcgca gcggctacta cagagaccaa accttcatgc tcgccgttgg gttactgacc
     1681 ttcagggagt tctttgggca gctgtacgcc aacaccgacc gtagtgagga tcccgggagc
     1741 atggggcggc agatggggag ccacttcgcc actcgattca tcgatgacga tggggccttc
     1801 atacctcagg tctcggcaaa gcactcggcc gccgacctct cgcccactgc ggggcagatg
     1861 gctcgtctgc ttggactggc ctatgcctcg aagctctatc ggaataaccc cgcacttgcc
     1921 ggttccactg acttcaccaa cggcggcaac gagatcgctt tcggcaccat cgggaacgcc
     1981 gcaaccagcg agggcatctt cttcgagacg atgaatgcgg cgggcgttct tcaggtgccc
     2041 atcttgatgt
//
LOCUS       DATDOZ010000284         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1561839, whole genome shotgun sequence.
ACCESSION   DATDOZ010000284 DATDOZ010000000
VERSION     DATDOZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561839"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VI541_05720"
     CDS             complement(<1..875)
                     /locus_tag="VI541_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF70438.1"
                     /translation="MVRSEATKETVLEQISVLIAEDDTTVREALADLIDAEESLQLIG
                     SAKDASEAIDLARKYRPDVALVDVKMPGGGGSRAAREIIISCPSTRVIALSAYDDRMT
                     IVELLRAGASGYLVKGESTDVLETIRRAMRGEVAISAELTTGVIRELLSLIERSEQVA
                     KDLEELGLEKATHLGQMQATVFERLASISTAARQLEQLSPALVGRIKAATAQLTRLAQ
                     RMTATNRLETRGVEISLQPVPVNDLMKAVRRAFPESHGRITLALDVDDALECWADPDL
                     LLTTLVAAVETVLVD"
     gene            952..1995
                     /locus_tag="VI541_05725"
     CDS             952..1995
                     /locus_tag="VI541_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLF70439.1"
                     /translation="MSEPRHGRGVYSSGDPIIDQQLRALVGDMDPQDADLVFELLATS
                     IRLGKDPVSRLDRKVAASALKEMRYAFSIFARYRDVRKVTIFGSARTAKGDPEYELAR
                     EFSQRIADRDWMVVTGAGPGIMQAAQEGAGGARSFGVNILLPFEMQANPVIAGDPKLI
                     NFKYFFTRKLMFVKEADAFLLLPGGFGTMDEAFELLTLVQTGKSDLHPIVMLDPPGGT
                     YWHGFENYIRKELLSRGYIYESDMSLFRHTDSVEVALQEIERFFRVYHSQRYVKGRLI
                     LRLKLMPSDDQISDMSERYSDMLAGPIEKAEASPAEVRDQDVPHLPRLALDFDRSHIG
                     RLRMLIDDLNEIT"
     gene            2037..3005
                     /locus_tag="VI541_05730"
     CDS             2037..3005
                     /locus_tag="VI541_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HLF70440.1"
                     /translation="MTTRKMLAAISVVSTMLSVFPGTYAQASPTRATVQEQLDKLTTQ
                     IAIVDEEFNVARLDLARVEGKIREVQYTKASADSRVDALRKTASARAAAVYRAGVPDV
                     MAVLLSSKDLGEFNRKMTVIGRVDGWESDVVSSLQIAGARADEIQADLQARRTRARAI
                     RDAIVSKKKILEQSAAKQRQLLSTLTTPRRARVAQASVFPTNLPVSGSARTAVQAAYA
                     QIGKPYKWGAAGPNSFDCSGLMLYAWGQAGVSLPHSSRAQFSATPRVARDALQPGDLV
                     FFGSPIHHVGMYVGGGNMVNSPETGEFVGVRSMNRRDYVGAGRPGI"
BASE COUNT          604 a          890 c          913 g          644 t
ORIGIN      
        1 tcatcgacca ggacggtttc gacggctgct acgagcgttg ttagcaagag atcgggatcg
       61 gcccagcatt ccagagcatc gtctacatca agagccaggg ttatccgtcc gtgcgattcc
      121 gggaatgccc tgcgtaccgc cttcatgagg tcgttgaccg gcacaggctg aagcgagatc
      181 tccacccctc tcgtttcgag gcgattcgtg gccgtcatcc tttgggccag gcgcgtcagt
      241 tgggccgtag cggcttttat cctgcccacg agggcagggc tcaactgttc cagttgacgc
      301 gcggcggttg agatcgaagc cagtcgctcg aaaacggttg cctgcatctg cccaagatgc
      361 gtcgccttct ctaagccgag ttcttctagg tctttcgcta cctgttcgga tcgctcgatc
      421 agcgacagta gttcccttat cactcctgtc gtcagttccg cagagatggc gacttcgccc
      481 cgcatcgcac gcctgatcgt ttcgagcacg tccgttgact cgcctttgac caggtatccc
      541 gaggccccag ccctcagcaa ctcaacgatg gtcatccggt cgtcgtaggc cgatagtgcg
      601 atgactctgg tggaggggca gctgatgatg atctcgcggg ccgcacgact gcctccgcct
      661 ccgggcatct ttacatccac gagtgcgaca tctggtctgt actttcgtgc gaggtcgatc
      721 gcttctgagg catccttcgc tgatccgatc aattgaaggc tctcctcagc gtcgatgagg
      781 tcggcaaggg cctcacgcac ggttgtgtcg tcttcggcga tcaacacgct tatctgttcc
      841 agaaccgtct ccttcgttgc ttcggaccga accatcatag gaggagaact tacgtaagcg
      901 atgcatatct tccgctactg aacccctgtg acggcatagc atctgttagg gatgagcgaa
      961 ccacggcatg gcagaggcgt ttattcatcg ggagatccga tcatcgatca gcaactccgt
     1021 gctctggtgg gcgacatgga tcctcaggat gcggaccttg tcttcgaatt gctcgccaca
     1081 tcgatccggc tcggtaaaga tcccgtatct cgtttggatc gcaaggtcgc tgcctccgcc
     1141 ttgaaggaga tgaggtatgc attcagcatc ttcgcccgct atcgcgatgt caggaaagtg
     1201 acgatcttcg gttcggcccg cacggccaag ggagaccccg aatacgagtt ggcacgtgag
     1261 ttctcgcagc ggatcgccga ccgcgattgg atggtcgtca cgggagccgg gcctggcatc
     1321 atgcaggccg cacaggaggg agccggaggt gctcgatcgt tcggcgtcaa catcttgctc
     1381 ccattcgaga tgcaggcgaa cccggtcatc gcgggcgacc cgaaactcat caacttcaag
     1441 tacttcttca ctcgcaagct catgttcgtc aaggaggcgg atgccttcct cttgctgccc
     1501 gggggcttcg gaacgatgga tgaggcgttc gaacttctga ctctcgttca aacgggaaag
     1561 agcgaccttc accccatcgt catgctcgac cctccgggag gaacctattg gcacggattc
     1621 gagaactaca tccgcaagga gctgttatcg cgcgggtaca tctacgagag cgacatgagt
     1681 ctcttcaggc acaccgacag cgttgaggtc gcgcttcaag agatcgaacg attcttccgc
     1741 gtgtatcact cgcagagata cgttaagggc agattgatac ttcggttgaa gttgatgccg
     1801 tccgacgatc agatcagtga tatgtcggag cgatattcgg acatgctcgc cggacctatc
     1861 gaaaaggcgg aagcatcgcc tgcggaggtg cgcgatcagg atgttcccca tcttccgcgg
     1921 ctcgcacttg acttcgaccg gtcgcacatc ggcaggctca ggatgctgat cgacgacctg
     1981 aatgagatca cgtgactggt ggatgtttgt ccaagggtcg gttagcatgc ctcgaaatga
     2041 cgaccagaaa gatgctggcc gccatcagcg tggtatccac catgctatcg gtcttcccag
     2101 gcacatacgc acaggcttcc cccacgcgcg ccacagtgca ggaacaactc gacaagctga
     2161 ccacgcagat agccatcgtt gatgaggagt tcaacgtagc gcgactggac ctggcgagag
     2221 tcgaaggaaa gatccgcgaa gtgcagtaca cgaaggcttc tgcggattct cgtgtggacg
     2281 cacttcgaaa gaccgcatcg gccagagccg cagccgtcta tcgggctggg gtccccgatg
     2341 tgatggcggt gctcctctcg tcgaaggatc tgggcgagtt caatcgaaag atgaccgtca
     2401 tcggtcgcgt cgatggctgg gaatcggacg ttgtgtcctc actgcagatc gccggtgcta
     2461 gggcagacga gatacaggcc gatctccagg cgcggcgtac aagggcccgc gcgatacggg
     2521 acgccatcgt ctcgaagaag aagatcctgg aacagagcgc cgctaagcag cgccaacttc
     2581 tatcaacctt gacgacgccc cggcgggcgc gcgtcgcgca ggcgtcggtg ttcccgacga
     2641 accttcccgt gtcgggatct gcgaggacgg cagttcaggc tgcctatgcg cagataggca
     2701 agccctataa gtggggcgct gcgggtccaa acagtttcga ctgctccggt ctcatgctgt
     2761 acgcgtgggg gcaggcgggc gtgtcgttgc cgcactcttc gcgggcgcag ttctcggcca
     2821 ctccacgggt tgcgcgcgac gccttgcagc caggcgatct cgtcttcttc ggttctccga
     2881 tccaccacgt cggcatgtat gtggggggcg gcaacatggt caactcccca gaaaccggtg
     2941 agttcgtcgg ggtcaggtcg atgaatagac gcgactacgt gggtgcggga agaccgggca
     3001 tctaaaggct cggacatcta gactgaggcc gactctctcg agaccagggg g
//
LOCUS       DATDOZ010000285         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3625277, whole genome shotgun sequence.
ACCESSION   DATDOZ010000285 DATDOZ010000000
VERSION     DATDOZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625277"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VI541_05735"
     CDS             complement(<1..158)
                     /locus_tag="VI541_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HLF70441.1"
                     /translation="MSDKRLPVVAVIGRPNVGKSTLVNRLAEVRSSIVGPMSGLTRDR
                     LDVDASWRG"
     gene            complement(155..802)
                     /gene="cmk"
                     /locus_tag="VI541_05740"
     CDS             complement(155..802)
                     /gene="cmk"
                     /locus_tag="VI541_05740"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HLF70442.1"
                     /translation="MSSQRLVIAIDGPAGSGKTTVARTLATILGVPYVDTGAMYRAFT
                     LKLLRTGSIEEVDSWVPLLDETKIESIGGLTMLDSDDVTREIRDHSVDSEVSRVAANP
                     AVRAWMVSRQRAEVGPQGVVMEGRDIGTVVLPDAFLKVYLTADPSVRAQRRATQNDSE
                     AVDSTLESIEERDRRDSIRSASPLKQAEDAILVDTTGKDVDEVVAHIMSLIAASR"
     gene            complement(799..2061)
                     /gene="aroA"
                     /locus_tag="VI541_05745"
     CDS             complement(799..2061)
                     /gene="aroA"
                     /locus_tag="VI541_05745"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HLF70443.1"
                     /translation="MTDRRFPPSLPLRGTLRMPGDKSISHRALILGSMAAGPSRIRGL
                     ADGLDVASTRRCITDLGAEATGGPADVIVKGWSQGPLDRLELDCGNSGTTMRLLAGVL
                     AWAPGKRVMRGDESLARRPMLRIVDPLREMGARIESDAGHAPLTIEGGPLHGIDHTPS
                     APSSQVKSCVLLAGMGAEGATSVTELSRTRDHTERMLKQMGARIHETPDRITIEAGPL
                     SPLDCEVPGDVSSAAFLIVAALLIPGSELRIEGVGLNETRTGFLRVLARMGADILIEV
                     ETTEPEPVGAIEVRSSRLVATHIAGRELPGLDEVPILALAGAFAEGATTFIDVGDLRH
                     KESDRVSSTCEMLRTLGVEVQEHPEGLVVHGGARPAGGAVDPAQDHRIALTAAVAGMA
                     SRDGVVVADWESASVSYPGFESSIGSVT"
     gene            complement(2064..>3110)
                     /locus_tag="VI541_05750"
     CDS             complement(2064..>3110)
                     /locus_tag="VI541_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HLF70444.1"
                     /translation="VGLAAAGTVERIVGFDLDPATSRRALELGAVHIAATSAAQAVEQ
                     ADLVVVATPVGSIPQTFASVKTYLRSGAIVTDVGSTKSGIVEEISVLISPDVHFIGGH
                     PMAGSEREGIEAATADLFRDSYWILTPTSQTDAAAYSSLVRFITGLGAKVLSLDADRH
                     DEAVALTSHLPQILASTLMRFAADVAGAEGGLPLLASGGFRDMTRIAGSSPALWMGIL
                     KENSAALVDVLGRYSGSLESVRAAIDTGDWNVVSKMLTDARSARSTLAAKSGRKSGDL
                     VEVRITVPDRPGVLADVATSLGEASINIEDLDIQHSPEGGRGILRILVEASDADATRS
                     ILVGRGFDVRAEEP"
BASE COUNT          608 a         1043 c          877 g          582 t
ORIGIN      
        1 ccccgccatg acgcatcgac atcgagacgg tctcgcgtca ggcccgacat cgggccaacg
       61 atggagctgc ggacctcagc tagacgattc acgagggtcg acttaccgac attagggcga
      121 ccgatgactg ccaccaccgg cagacgcttg tcgctcatct cgacgctgcg atcagagaca
      181 tgatgtgagc cactacctca tcgacgtcct tgccggttgt atccaccaag atcgcatcct
      241 cggcctgctt cagtggggaa gccgacctta tcgagtcacg tctgtctcgc tcctcgatac
      301 tctccaacgt cgagtcgaca gcctcactgt cgttctgagt tgcccttcgt tgcgcccgaa
      361 cgctcggatc tgcggtgagg tagaccttga ggaaagcgtc gggcagcacc accgttccga
      421 tatctcttcc ctccataacc acaccctggg ggccgacctc ggcccgctgc cgtgacacca
      481 tccaagcgcg gaccgcaggg ttggccgcga cccgggacac ttctgagtcg acggagtgat
      541 ctctgatctc acgggtcacg tcatcgctgt cgagcatcgt caggcccccg atcgactcga
      601 tcttcgtttc atcgagtaag gggacccatg agtcgacctc ttctatcgaa ccggtcctca
      661 gcaacttcaa tgtgaatgcg cggtacatcg caccggtatc gacatatgga actccgagta
      721 tggtcgcaag ggtgcgcgcg accgtcgtct tgcccgaccc agccgggccg tctatcgcga
      781 tcaccaatct ctgtgaactc acgtgacact tcctatcgat gactcgaaac ccgggtaaga
      841 caccgaagcg ctctcccagt ccgcaaccac gaccccatcg cgagatgcca tgccggcgac
      901 cgctgccgtt agggcgatcc gatgatcctg agctggatcg acggctccac ccgcggggcg
      961 cgctccccca tggaccacta gaccttcggg atgctcctgc acttctacac cgagtgtacg
     1021 caacatctca caggtcgagc tgacgcgatc gctctccttg tgccgaagat cgcctacatc
     1081 gatgaaagtc gtagctcctt ctgcgaaggc gccagcgaga gcgaggatcg gaacctcgtc
     1141 cagaccgggg agttccctgc ccgcgatatg cgtcgccacg agtctcgaag accgcacctc
     1201 gatcgcgccc acaggctcgg gctcagtggt ctcaacctcg atcaggatgt cggcacccat
     1261 gcgcgccagg acccgcagga aaccggtgcg ggtctcattc aacccgaccc cttcgatgcg
     1321 aagctccgag ccggggatga gcagcgccgc cacgatcaag aaggccgcgg atgaaacatc
     1381 ccctgggacc tcgcaatcga gtggcgagag agggcctgct tcgatggtta tgcggtcggg
     1441 agtctcgtgg atgcgagccc ccatctgctt caacatcctc tccgtgtggt cgcgggttct
     1501 agaaagttcg gtcactgaag tagcgccctc ggcccccatg cccgcgagaa ggacgcatga
     1561 cttcacctgc gaactcggcg ctgaaggcgt gtggtctatc ccgtgaagtg gcccaccctc
     1621 gatcgtcagc ggtgcatgac ctgcgtctga ctcgatccgc gcccccatct cccgcagagg
     1681 atccactatc cgcagcatgg gtctgcgcgc cagagattca tcaccgcgca tcaccctctt
     1741 gcccggtgcc catgcaagga ccccggcgag aagacgcatg gtcgtaccgg agttgccgca
     1801 gtccaactcc agtcgatcga gcggcccctg cgaccaaccc ttcacgatca cgtcggcggg
     1861 gcccccagtg gcttcggctc ccagatccgt gatgcatctt cgcgtcgatg cgacatccaa
     1921 cccatcggcc aaaccacgta tcctgctcgg cccggcagcc atcgagccga ggatcaatgc
     1981 tcggtgcgag atggatttgt ctccaggcat gcggagcgtt ccccgtagcg gcagcgaagg
     2041 gggaaagcgt cgatcagtca ctatcaaggt tcctctgcgc gaacatcgaa acccctacct
     2101 acaaggatgg acctcgtggc atccgcatcc gatgcctcca ctaggatccg caggatccct
     2161 cggcccccct caggcgaatg ttggatgtcg aggtcctcga tgttgatgct cgcctctccg
     2221 agactggtag caacgtcggc gagaactccc ggacgatcgg ggaccgtgat cctcacctca
     2281 acgagatccc ctgacttcct tccagactta gcggcaagag tgctcctcgc cgacctcgcg
     2341 tccgtgagca tcttggaaac gacgttccag tcgccagtat cgatagcggc cctaaccgac
     2401 tcgagggatc ccgaatagcg tccgaggaca tcgaccaacg ccgccgagtt ctccttcagt
     2461 atccccatcc agagggctgg tgaactcccc gcgatgcgcg tcatgtccct gaacccgccc
     2521 gaagccagca gaggaagtcc cccctctgcc cccgcaacat ctgcggcgaa cctcatcaat
     2581 gtgctggcaa ggatctgggg aaggtgactc gtcagtgcga ccgcctcgtc atgacgatcc
     2641 gcatcgagcg agaggacctt cgcgccgaga cccgtgatga accgaaccag cgagctgtaa
     2701 gcggctgcgt cggtctgaga ggtgggggtc aggatccagt aactgtctcg aaagaggtcg
     2761 gcggtggcag cttctatccc ttcgcgttcg gagcctgcca tcggatgtcc gccgatgaag
     2821 tgaacatcgg gtgagatcag taccgagatc tcttcgacga tcccgctctt ggtgcttccg
     2881 acgtcggtaa cgatggcacc actgcgaagg tacgttttca ccgacgcgaa tgtttgcggg
     2941 atgcttccaa ccggcgttgc cacgacaaca agatcggcct gctcgactgc ctgcgccgcc
     3001 gaagtagcag cgatatgcac ggcccccaac tcgagggctc ttcgtgatgt tgcgggatcg
     3061 agatcgaatc cgacgatacg ttcgaccgtc ccggccgcgg cgagcccgac
//
LOCUS       DATDOZ010000286         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1301686, whole genome shotgun sequence.
ACCESSION   DATDOZ010000286 DATDOZ010000000
VERSION     DATDOZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301686"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VI541_05755"
     CDS             <1..346
                     /locus_tag="VI541_05755"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLF70445.1"
                     /translation="DIRSPGTVANLRQNPSVEINVVDPLSRKGYRFKGTAQVLEQGLD
                     FESGIAFFRERGVTSPIRSVVMVRITRALAVTSPAYDAGASESEIRARWSEFYGRQPH
                     GNTSSNSDAIDG"
     gene            427..837
                     /locus_tag="VI541_05760"
     CDS             427..837
                     /locus_tag="VI541_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLF70446.1"
                     /translation="MIALFVIRRDDSILVFEAFDSAMGSPFYRPLGGGVKRGETSEAA
                     IVREIREELSLEIMDLHLLGTLENIFTYEGELGHEAVFVYEGRFADTASYAVEEFTVT
                     EDDGSALSARWIPLQFFDDYHRLVPRGLKELIDA"
     gene            940..1335
                     /locus_tag="VI541_05765"
     CDS             940..1335
                     /locus_tag="VI541_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF70447.1"
                     /translation="MKVRSLGWLGVRTANASAMCTFYRDVLGLEVLQEQSTGTRFRLP
                     DGTEAHVYDADDTDHDFFGSGPVVGFFVESFAAARATMAESGIEFLYPEPQRHGGRVW
                     QHFRAPDGNVYEIIGLDDLDTINPRLGSA"
     gene            1383..2168
                     /locus_tag="VI541_05770"
     CDS             1383..2168
                     /locus_tag="VI541_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HLF70448.1"
                     /translation="MSYETLIYDLAGGVATITLNRPEKRNAVNRTMFEELEAVFDEIA
                     ASSEVRVFVLQGAGDHFCSGADLSTLDEAPKTPFQMLARMEEIHRIMRKIVYCPKPGI
                     AAVRGYAAGGGANLALACDLVVMADDAKFAELFVKRGLVVDMSGTFTLPRTVGLHRAK
                     ELALLGDTIDAARAYEFGIANRVVPVDELEITVKDLASRLAAGPPIALSFMKKALNDS
                     FAQTFDEVLSGEAHHQAAIFSTHDFSEAISAFFEKRTPSFTGE"
     gene            2201..2986
                     /locus_tag="VI541_05775"
     CDS             2201..2986
                     /locus_tag="VI541_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70449.1"
                     /translation="MALILASFAAALGWVLLDSRRVVGSVPGDAPILGLAAVKSGFAV
                     ATEKGVYFSSDGETWSKVAKFGSKPSLATSGADDAFIWSDRILAKTSDLATFDVGAGS
                     LLPAVAISVDSAGNIYVAEDPRHYALVGPDGGLQRITAGPGPQDVIAIAGIPGEPVTL
                     LVGGLNSGLWRSTDGGLHWRQILLTPTRALLIDPSNAKKVLLGTAGGVLYSRDGGLRW
                     FFSDLRSSVEALSASGGRFYALTQDRLLYSSEDGIQGWARFPN"
BASE COUNT          605 a          931 c          975 g          657 t
ORIGIN      
        1 cgacatccgc tccccgggaa ccgttgccaa cctgaggcag aatccgtcgg tagagatcaa
       61 tgttgtcgat ccattgtcgc gaaaggggta tcggttcaag ggcactgcgc aggtcctcga
      121 gcaaggtctc gactttgagt cgggcatcgc ctttttcaga gagcgcggcg tgacgagccc
      181 gatccgaagt gtcgtcatgg ttcgtatcac ccgcgcgttg gccgtgacct ctccggctta
      241 cgatgcagga gcaagcgaga gtgagattcg cgcccgttgg agtgagttct acgggaggca
      301 gccacacgga aatacaagta gcaatagcga tgccatcgat ggttgactcg tgcaaccgcc
      361 tctcgccacg ctcgccatgg ccgacgcgcg agtccgctgg gaccataaat gagtgaaaac
      421 attcgcgtga tagccctctt cgtcatcaga cgcgacgatt cgatactcgt cttcgaggca
      481 ttcgattcgg cgatgggttc gccgttctat cgacctcttg gaggcggggt caagcggggg
      541 gaaacatccg aggcagcaat tgtccgcgag atccgagagg agctaagcct tgagatcatg
      601 gacctccatc tcctaggcac gttggagaat atcttcacct atgagggaga gttgggacac
      661 gaggcagtat tcgtttacga gggtagattt gctgacacgg cgtcgtacgc cgttgaggaa
      721 ttcacggtca cagaagacga tggcagcgca ctttccgcgc gctggatacc tcttcagttc
      781 ttcgacgatt accatcgact tgtcccgaga ggcctgaagg agctcataga tgcgtagcaa
      841 gctggccaaa cagctgcata accagacgtt ggacccgacc gcaatagacg ggcgtagcta
      901 ggctcgcaca atgagacgtt ttcgagactg aaggtcgaca tgaaagtacg ttcactcggt
      961 tggctcggag ttcggacggc aaatgcctct gcgatgtgca ctttctatcg tgacgtccta
     1021 ggtcttgagg tcctacagga acagtcaact ggcacaaggt tccgcctacc tgatggcaca
     1081 gaagcgcatg tctatgacgc cgacgacacc gatcacgatt tcttcgggtc cggcccggtc
     1141 gtgggattct tcgttgagtc tttcgcagcc gcccgggcga ccatggctga atccgggatc
     1201 gaattcctgt accccgagcc gcagcgccat ggtggtcggg tttggcagca cttccgcgct
     1261 ccagacggca acgtctacga gatcatcggc cttgatgacc tcgacacgat caaccctcgg
     1321 cttggctcgg cgtaacaaca tcgcggatcg cgaaatagga tggctgaaac caaggaggag
     1381 agatgtccta cgagaccctg atctatgacc tcgccggggg agtcgcaacg atcacgctga
     1441 accgtcccga gaagcgcaac gccgtcaacc gcacgatgtt tgaggagctg gaggccgtat
     1501 tcgacgagat cgctgcctca agcgaggtca gggtcttcgt tcttcaggga gcgggcgatc
     1561 acttctgctc gggggccgac ctgtcgacat tggacgaggc ccccaagacg ccgttccaga
     1621 tgctcgctcg catggaagag atccaccgca tcatgcgtaa gatcgtctac tgcccgaagc
     1681 cgggtatcgc cgcggtccgc ggatatgcgg ccgggggcgg cgcgaacctg gcgctcgcat
     1741 gcgacctcgt agtcatggcc gatgatgcga agttcgctga actcttcgtg aagcgcgggc
     1801 tcgtggtcga catgagcgga actttcacgc tcccgcgcac tgtagggctt caccgcgcca
     1861 aggaactcgc tctgcttggc gacaccatcg atgccgcgcg cgcatatgag ttcggtatcg
     1921 ccaacagagt ggtgccagtc gatgagttgg agataaccgt caaagacctg gcgtcacgac
     1981 tcgcggctgg gcccccgatc gctctctcat tcatgaagaa ggcactgaac gattctttcg
     2041 ctcagacctt cgacgaggtc ctttcgggcg aggctcacca tcaggccgcg atcttttcga
     2101 cgcatgactt ttctgaggcc atctcggcgt tcttcgagaa gcggaccccc agcttcaccg
     2161 gagagtgaca ccaccgcgac gccgctggcc gatcgttctc ttggccttga tcttggcctc
     2221 tttcgctgcg gctcttgggt gggtgctact cgatagtcgt cgcgtcgtgg gctcagtccc
     2281 cggtgacgct cccatactcg gtctcgccgc cgtgaagagt ggcttcgccg tcgccaccga
     2341 gaagggcgtc tacttcagtt ccgacggaga gacctggtcg aaggtcgcca agtttggttc
     2401 caagccatcg ctcgctacta gcggcgccga tgatgcattc atctggtctg accggatcct
     2461 ggctaagacg agcgatctcg caacattcga tgtgggggct ggctctctcc tgcccgctgt
     2521 agccatctcg gttgattcgg ccgggaacat ctatgtggcc gaggacccgc ggcactatgc
     2581 cctcgtcggt ccggacgggg gcctccagcg gatcacagcg ggccccggcc cccaggatgt
     2641 catcgccatc gcgggtatac cgggggagcc cgtcaccctc ttggtcgggg ggctcaactc
     2701 gggactatgg cgctcgaccg acgggggtct tcactggcgg cagatcctgc tgactccaac
     2761 gcgtgctctg ctgatagacc cttcgaatgc gaagaaggtc ctgctcggaa cggcaggcgg
     2821 ggttctctat tcgcgagatg gagggctgcg ctggttcttc agcgacctgc gctcgtcggt
     2881 agaggccctc tcagcgagtg ggggcaggtt ctatgccctg acccaggatc gcctgctcta
     2941 ctcgtcggaa gacggcatcc agggctgggc gcggttcccg aactgagagg tctgttcgcg
     3001 aaaaagtacc tcacaggtgg ctggttacat aaagaccatg gtggtgccca gaatgtcgta
     3061 ccccgcgagt agcctccggg catgctatct aaggtctcat cggttgccgt tgttgggatg
     3121 gatgcgcgcc tcgtcgaggt cgaggtcgcg ctcggctcgg gggtcccc
//
LOCUS       DATDOZ010000287         2864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1301825, whole genome shotgun sequence.
ACCESSION   DATDOZ010000287 DATDOZ010000000
VERSION     DATDOZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301825"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1080
                     /locus_tag="VI541_05780"
     CDS             115..1080
                     /locus_tag="VI541_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HLF70450.1"
                     /translation="MRRVLILVILPFALSACLNRSGTITLSADFNDVGDLVRGAGVQT
                     SDIKIGSVTGIELEGTHARVTMRISDKTPLPSNTGAVVRSTSLLGEKFVDLRPPVAGK
                     QGRLRDGSRIPLSRTGRAAGLDDALIKLGQILEGAEPEDLGIFIHSAATIVDGKQDQI
                     GRVLEQLRLLTDTVAQHRTDIGASVDNLGTALDTLADGSATISSALKSGAAATRILGD
                     QQGDLDRLLVSLDRFASVSADLMSATHTSTDASLKDLRLILDKVMLTTDDLSRTLTSL
                     ATFTHLWPKAIPGDYIQLDQVQEIGSAIGPEQTSSSFIANIWRAP"
     gene            1077..2216
                     /locus_tag="VI541_05785"
     CDS             1077..2216
                     /locus_tag="VI541_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HLF70451.1"
                     /translation="MRVTRRVIIRMTAFIVGAIALVYILALQILPTVVGSTYTISGIF
                     PEAGGVFTAQEITYRGVQIGRVGAMTLTRNAVKIEMIIDSKYKVPADGTRAEVLYKSA
                     VGEQFIDLVPSGVGPPYLEQGDVIGRERTQIPIQTEDLLRHLDGLLRSVDARALGVVV
                     HELGTALADHGEDLEKILLALDTLSAIGAERKNEIAGGIEAGSSLQRAFNATSEDFVS
                     GAGSLSEIATILGKRRAELKATLDATESLDRSILRLLQDRETDIKDLIANLGSSTRAL
                     HEQRPQLDLTLQFLAPFLNDAYRAYRAPYFVFNLVKNSENKACAYEPSSEHRPITEVN
                     PGTFGNPEDPQTDFACRGGDPPEAGSSMTRPAKAVSWPVDQRGPR"
     gene            2396..2860
                     /locus_tag="VI541_05790"
     CDS             2396..2860
                     /locus_tag="VI541_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70452.1"
                     /translation="MRIVSAALALAIAVIVFLSVSLYRLGSIEQDRKAAIKAARTAAL
                     ALTNFDYKTIDEDISKILQTSSASFRREYARTLTGTDFRDSIIEAKGSSSGEIKTVAV
                     TSSDHEGAQILVVVQQKRTNSAASGPASQEVRIFMTMIKTPSGWKIDRLEKL"
BASE COUNT          606 a          904 c          833 g          521 t
ORIGIN      
        1 cggcgggacc accacccacg atctcgaggt ccctcgggac tccatctccg agcggatcct
       61 cggactcttc atgcggttcg ggggcctgat agacggggcg atcgggggcc tgagatgaga
      121 cgagtcctca tcctcgtgat cctgccgttc gccctttccg cttgcctgaa ccgatccggc
      181 acgatcacgc tgtcggccga cttcaatgac gttggcgacc tcgtccgtgg agcgggggtg
      241 cagaccagcg acatcaagat cggctctgta actgggatcg aactcgaagg tacgcacgcg
      301 cgggtcacca tgcggatatc tgacaagacg cctcttccct ccaacaccgg ggccgtagtc
      361 agatccactt ccttgttggg agagaagttc gtcgaccttc ggcccccggt cgcgggaaaa
      421 cagggcagac tgcgcgatgg cagccgcatc ccgctttcga ggaccgggcg tgcggcgggg
      481 ctggatgatg cactcatcaa gctcggtcag atcctcgagg gggccgagcc cgaagacctg
      541 gggatcttca tccactcggc ggcaaccatc gtcgacggga aacaggatca gatcgggagg
      601 gtgctcgagc agctacggct actcaccgac accgtcgcac aacatcgcac cgacatcgga
      661 gcttcagtcg acaacctcgg aacggctctg gatactctcg ccgatggttc cgcgaccatc
      721 tcttcggcgt tgaagtcggg cgctgccgcc acaaggatcc tgggcgatca gcagggggac
      781 cttgatcgac tgctcgtctc gctcgaccgc ttcgcctctg tttctgcaga tctgatgtcg
      841 gcaacgcaca catccaccga tgcttctctc aaagaccttc gtctgatcct ggacaaggtg
      901 atgctgacca cggatgacct cagcaggacg ctcacctcgc tcgcaacatt cacgcacctg
      961 tggcccaagg cgataccggg tgactacatc caactcgatc aagttcagga gatcggaagc
     1021 gccatcgggc ctgaacagac gagttctagt ttcatcgcaa acatctggag agccccatga
     1081 gagtcactcg tcgagtcatc atcaggatga cagcgttcat cgtcggggcc atagccttgg
     1141 tttacatcct ggcgcttcag atcctgccga cagtcgtggg ctcgacatac acgatctcgg
     1201 ggatattccc cgaagccggc ggagtgttca cggcacaaga gatcacctat cgcggagtac
     1261 agatcggtcg cgttggggcc atgacgttga cgcgcaacgc ggtgaagatc gagatgatca
     1321 tcgactccaa gtacaaggtt ccagccgatg gcactagggc agaggttctc tacaagagcg
     1381 ccgtcggcga gcagttcatc gacctcgtgc catccggagt cggcccccca tatcttgagc
     1441 agggagacgt gatcgggcgg gagagaacac agatccccat ccagaccgag gatctgctcc
     1501 gacacttgga tgggcttctc aggtccgttg acgccagagc cttgggagtg gtcgttcacg
     1561 agctcggcac ggcactagcc gaccatggcg aggatctcga gaagatactt cttgccctcg
     1621 acaccctctc ggccataggg gccgagcgta agaacgagat agccggcggg atcgaagctg
     1681 gttcatcgtt gcagagagca ttcaacgcaa cctcagaaga cttcgtcagc ggcgctggct
     1741 cgctctcgga gatagcgacg atcctcggca aacgtcgggc tgaactgaaa gcgaccctag
     1801 atgccaccga atcgctcgac aggtcgatcc ttcggctgct gcaggaccgc gagaccgata
     1861 tcaaagacct gatcgcgaac ctcggctcat cgaccagggc actacatgag cagcggcccc
     1921 aactcgacct caccctccag ttcctggccc ccttcctgaa cgatgcatat cgcgcatatc
     1981 gggcgcccta cttcgtattc aacctagtca agaacagcga gaacaaagct tgcgcatacg
     2041 aaccatcatc ggaacatcgg cccatcaccg aggtgaaccc aggaactttc gggaaccccg
     2101 aagatcccca gaccgacttc gcctgcaggg gcggcgaccc gcccgaggcc ggctcatcta
     2161 tgacccgccc cgcaaaggcg gtatcgtggc ccgtcgacca acgggggccc cgatgaacga
     2221 tgacgacttc tggtctgctt cttccgaggc ggccaagagt cagcccagaa gtgacctcgg
     2281 ctttgctggt gacgactctc cagcgtcggc ctgggatgac cccgtaggcg aacgttctcg
     2341 ggcggaaaac ccccgcctac cggtggtttc tcgcccgagt tcgcagagta ggcgcttgcg
     2401 gatcgtatcg gcggcactcg cactagcgat cgccgtgatc gtctttctgt cggtctccct
     2461 ctatcggctc ggatcgatcg agcaggatcg gaaggcagcc atcaaggccg cccggacggc
     2521 cgctctcgcg ttgaccaact tcgactacaa gaccatcgac gaggacatca gcaagatcct
     2581 ccaaacgtcg agcgcttcct tccggaggga gtacgcgaga accctgacgg gcacggactt
     2641 ccgcgacagc atcatcgaag cgaaggggtc atcaagcggc gagatcaaga ccgttgcggt
     2701 cacatcgtca gaccacgagg gggcccagat actcgtcgtc gtgcagcaga aacgtacgaa
     2761 ctctgctgct tccggccccg cgtcgcagga ggtccggatc ttcatgacga tgatcaagac
     2821 gccctctggg tggaagatcg acaggctcga aaaactctag atgc
//
LOCUS       DATDOZ010000288         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3107128, whole genome shotgun sequence.
ACCESSION   DATDOZ010000288 DATDOZ010000000
VERSION     DATDOZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107128"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VI541_05795"
     CDS             complement(<1..969)
                     /locus_tag="VI541_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLF70453.1"
                     /translation="MRIESSVTSISWIPSEAVSGLTKLPFETGIAHYDDPPPEILEDL
                     EELRVNDRFRFANQLSAYVDVEDGIIVGYGSSGEGHIGSTTMRLAGRDMTFAAVALPD
                     IKPPAEVHKDRVRFVQTSGGRTGAPAPRRVSRKPFIQLAAPLAWTTLALTLHADGRSE
                     FEVVGASPFPRHWIYDHEGKLAAKSGLIDFKTWYRDAFGDATPWGSQDSPALVTAAET
                     ALERELSATIMSEGQAPKIRKLKVGAFLVKQGDLGTDLFLLLDGVLAVQVDGDDIAQV
                     GPGALLGERALLEEGVRTSSLRALTPVKVAVVAANQVSKTALLRIRT"
     gene            1125..2438
                     /locus_tag="VI541_05800"
     CDS             1125..2438
                     /locus_tag="VI541_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF70454.1"
                     /translation="MTDAQITIFLADDNLLVREGVRALLSLEPDFEIVGVAEDYDGLV
                     TGAESSAPQVIVTDIRMPPTFTREGIEAAQHVRKLHPGTGVVILSQYDEPEYAIALLS
                     EGASGYAYLLKDRVAEGDQLARAIREVASGGSMLDPKIVASLVSPVTQTGVLSASEEE
                     LLKLVAGGKPIKAIAVMLRTTPADVSAGVEALFLKLAQAASAGVEGALQRLKMLHQVI
                     VDREEVGETLSHFIPGGLAEKLRNEGHEIGRTEELTVTVLMSDIRGYSTIAESADPSS
                     LAMQLNEHRARMNRAIIDESGSVMQFVGDAVMAVFGAPIPANDHAERAVVAARAMHKA
                     QSEVNELWIARGLPKFHLGIGLSTGPVAAALLGSDERLEYTVVGDTVNLSQRLQQWAD
                     PGEIVLSENTYNACRDSVAGAIKMEPQQVKGRQAEVYAFKIPAFD"
     gene            complement(2435..3172)
                     /locus_tag="VI541_05805"
     CDS             complement(2435..3172)
                     /locus_tag="VI541_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF70455.1"
                     /translation="MGGGSRYIERSFLGRALNRYAGEVVIRLIFAEDNYLVREGVRLL
                     IEQEPDFEMVAVCEDYPALMAAVDELQPDVVLTDIRMPPTGTDEGIRAARALRESHPE
                     IGVVVLSQYDEPQYAISLLEHGSQGRAYLLKEGLSDMKQLAGAIRQVASGDSFIDPKV
                     VDALVSAKSRSESSPLSRLTPRESEILSVMAQGKNNAAIAETLYLTDRAVEKHINSIF
                     SKLNLSEEKEAHRRVKAVLLFLSEGGN"
     gene            complement(3148..>3443)
                     /locus_tag="VI541_05810"
     CDS             complement(3148..>3443)
                     /locus_tag="VI541_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF70456.1"
                     /translation="GFEVVGEAETGEDSIVKARDLHPDLIVMDVNLPGIDGTEATKRI
                     LGEMNTVVVLLVSTYERSEYEPRAAECGASAYIPKSEFGPDTLESAWEAAAGT"
BASE COUNT          637 a         1046 c         1075 g          685 t
ORIGIN      
        1 ggtccggata cgcagaaggg cggtcttgct cacctggttc gccgcgacca ccgctacctt
       61 caccggcgtg agggcccgta gcgatgaggt tcgaacgcct tcttcgagaa gagcgcgctc
      121 gccgagcagc gcccccggtc ctacctgcgc gatgtcgtcg ccgtcgacct gcaccgccag
      181 cactccgtcg agcagtagga agaggtcagt gcccaggtcg ccctgtttga cgaggaacgc
      241 tccgaccttg agcttgcgga tcttgggggc ctgtccttct gacatgatcg tggctgacag
      301 ttcgcgttca agtgcggtct cagctgcggt cacaagtgcc ggtgagtcct ggcttcccca
      361 tggtgtggca tcaccgaagg catctcgata ccaggtcttg aagtcgatca gcccactctt
      421 ggctgcgagc ttgccttcat ggtcgtagat ccagtgccgg gggaatgggc tcgcccctac
      481 gacctcgaac tcactgcgcc cgtctgcatg aagcgtgaga gccagagttg tccaggcaag
      541 gggggccgct agctggatga agggtttgcg agatacgcgg cgcggcgcgg gggcccccgt
      601 ccgccctcct gatgtctgga cgaaacgcac ccggtcctta tggacctcgg ccgggggctt
      661 gatgtcgggg agcgccaccg ctgcgaaggt catgtccctg ccagccaatc gcatggtggt
      721 cgagccgatg tggccctctc cggagctgcc atatccgacg atgattccat cctcgacatc
      781 cacatacgcg ctgagctggt tggcgaaccg gaagcgatcg ttcaccctaa gctcttcgag
      841 atcttcgagg atctcgggag gtgggtcatc gtagtgggct atccccgtct cgaagggaag
      901 cttggtgaga cctgaaactg cttcagacgg tatccacgag atcgacgtga cgcttgattc
      961 gatcctcatc tcctgccctc ctgatgtcgc gttcttgaag gctaaccccg gcgggccagc
     1021 atgactatgg ggccttcctc ccgatgcgcg gggatacacg tacgaaatag agtggagggg
     1081 tactcccact tgacccacga cgcgcgaggg ggccaaatac cttcatgacg gatgctcaga
     1141 tcactatctt cttggccgat gacaaccttc tcgttaggga gggcgtcaga gccctgcttt
     1201 cgctggaacc cgatttcgag atcgtcggcg tggccgagga ctacgacgga ctggtcacgg
     1261 gagccgagtc gtcggctcct caggtgatcg ttactgatat caggatgccg cctacgttca
     1321 cacgcgaggg catcgaagca gcccagcatg tacgcaagct gcatccgggg acgggagtgg
     1381 tcatcctctc tcaatacgat gaacccgagt atgcgatagc cctgctgagc gagggtgcgt
     1441 ccggatacgc gtacctgttg aaggatcgtg tcgccgaggg cgatcaactc gcccgcgcga
     1501 tacgcgaggt cgcttccggt ggttccatgc tcgaccccaa gatcgttgcg tcgctcgtct
     1561 cccctgtgac tcagacgggg gtcctatccg cgtccgaaga ggaactgctg aagttggtgg
     1621 caggaggcaa gcccatcaaa gcgatcgccg tgatgctgcg gacgacacct gccgatgtgt
     1681 cggcgggcgt cgaggctctc ttcctgaaac tcgcacaggc ggcaagcgct ggagttgagg
     1741 gggcccttca gagactgaag atgctgcacc aggtgatcgt cgaccgtgaa gaggtcggag
     1801 agacgttgag tcacttcatc cctgggggcc ttgccgagaa acttcgcaac gaaggacacg
     1861 agatcggtcg gaccgaggag ttgacggtga ccgttctgat gtccgatatc cgcggctact
     1921 cgacgatcgc tgagtcagcc gatccgagtt ctctcgcgat gcaactgaac gagcaccggg
     1981 cgcgcatgaa ccgagcgatc atcgacgagt cgggcagtgt gatgcagttc gtcggcgatg
     2041 cggtcatggc ggttttcggg gcccccatcc ccgcgaacga tcatgccgaa cgagccgtcg
     2101 tcgccgcaag ggcgatgcac aaggcccaga gcgaggtcaa cgaactgtgg atcgctcgtg
     2161 gtctgccgaa gttccatctg gggatcggac tgtcgacggg ccctgtcgcc gcggcattgc
     2221 tagggtcgga cgaaaggctg gagtacacgg tcgtgggcga cacggtgaac ctctctcagc
     2281 gactccagca gtgggcggac cccggcgaga tagttctcag cgagaacacc tataacgcgt
     2341 gtcgcgattc ggtcgccggt gccatcaaga tggagccgca gcaggtcaag ggccgccagg
     2401 ccgaggtgta cgcgttcaag atcccggcct tcgactagtt gccaccctct gagaggaaga
     2461 gaagcactgc tttgacgcga cgatgtgcct ccttctcttc ggacaggttg agtttcgaga
     2521 agatcgagtt gatatgtttc tcaaccgcac gatccgtcag gtagagggtc tccgcgatgg
     2581 ccgcgttgtt cttgccctgc gccataaccg agaggatctc gctctcacgt ggcgtcagtc
     2641 gcgagagggg cgagctctct gacctgctct tcgctgatac cagagcatcc accaccttcg
     2701 ggtcgatgaa tgagtcgccg gatgcgacct gccgtatggc acctgccagc tgcttcatat
     2761 cggagaggcc ctccttgaga aggtatgccc taccctgaga accatgttcg agcagtgaga
     2821 tggcgtactg gggttcgtca tactgactca gcacaacgac tccgatctcc gggtgactct
     2881 ctcggagcgc ccgagcggca cggatccctt cgtcggtccc ggttgggggc atccggatgt
     2941 cggtgaggac cacatcgggc tgcagttcat cgaccgctgc catcaaggcg gggtagtcct
     3001 cgcagaccgc caccatctcg aagtcgggct cctgttctat gaggagcctc acgccttcgc
     3061 gcacaaggta gttgtcttcg gcgaatatga gtcggatgac cacttcgcca gcataccgat
     3121 tgagcgcgcg gcctaggaaa cttctctcta tgtacctgct gccgcctccc acgccgactc
     3181 gagcgtgtcg ggaccgaact cggacttggg tatgtaagcc gaggccccac actcggcggc
     3241 acgcggctcg tactccgacc tctcataggt cgagacgagc agcaccacga cagtgttcat
     3301 ctcgccaagg atccgcttgg tcgcctcggt gccgtcgatc cctggcaggt tgacatccat
     3361 cacgatcagg tcggggtgca ggtcccgtgc cttcacgatc gagtcctctc cagtctcggc
     3421 ctcgccgacg acctcgaacc cgg
//
LOCUS       DATDOZ010000289         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2594007, whole genome shotgun sequence.
ACCESSION   DATDOZ010000289 DATDOZ010000000
VERSION     DATDOZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594007"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VI541_05815"
     CDS             complement(<1..421)
                     /locus_tag="VI541_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLF70457.1"
                     /translation="MHPSEAEPTPASFIGRVARSGAWNVVAYVVDAGSALGISVLVGR
                     ALGPATFGRYAYLMWIIRTVAVLGAIGLPAAATRMVSEFLGADDAPRATGLYRTALRI
                     HLVTTPLLFLIAGSFVWIKGGHDPVLAIAMGLATVFLS"
     gene            complement(431..1294)
                     /locus_tag="VI541_05820"
     CDS             complement(431..1294)
                     /locus_tag="VI541_05820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF70458.1"
                     /translation="MTISSLVPAKEFEESVKHFPSTLGWLSTNAAQIFYAFHTFQLRN
                     LKMSGDVLEVGAHEARSSVFFTNLLRDGETLHVSDLFDQQDLNISKSAGSSYAAWERN
                     FERFKSGDPNVVIHHGPSQNLDPVALGSNFRFAYLDGGHATDEVKADLELAEAILVEG
                     GVAVVDDTFNQDFPGTTEGVIAYCYKDDSKLVPLLCFETKVALVNKSYYETYRSMLTE
                     MLDQGEHWFLPPQLLCGHEVLTIRFAGRWEVRSKVLKRNFPLAHQVLRSTKIAQNVFH
                     RLRGALSDRAK"
     gene            complement(1291..>2318)
                     /locus_tag="VI541_05825"
     CDS             complement(1291..>2318)
                     /locus_tag="VI541_05825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF70459.1"
                     /translation="SIRVIEFPENRRPAAARNAGIQAAQGRYIALLDADDVWMPKRLE
                     VLVGFLEHDHTYSIATSDSYLLIDGVRTQRSWFGTTGTIFYADDQNVNIFRGNYLSNL
                     LVYRKRIFDTYGLFDEGLWCEDWDRNIACLVGGERAAFIPEPLAYYRVTPGSLSKSHL
                     LMSRDSKMILDRWLETGLDPAARRIARASRTRFEWDLVKAELLAGHRRSVLGRLLRLD
                     LSAPGDVRIKALLWTFLPAALIGRIHMRRSRFLAENGGPWSLDEALKVIASGDDAGAV
                     RYVTAVVLFGYPLTIRMLALSWLGLPPLRKFLLSKLLAESRARAGRQQPLVGHQSLGS
                     EERKGID"
BASE COUNT          523 a          689 c          656 g          450 t
ORIGIN      
        1 gactcagaaa aacagttgcc agccccatgg cgatcgcaag gaccggatca tgcccgccct
       61 tgatccagac gaaggaacca gcaatcaaga agagaagtgg agtggtaacc aggtgtatcc
      121 gcaaggccgt tcgatagagc ccggtcgcgc gaggcgcatc atccgctccc aggaactccg
      181 agaccatgcg cgttgctgcc gccgggagac cgatcgcgcc aagcacggca accgttcgga
      241 tgatccacat gaggtacgca tatcggccga aggtcgctgg cccgagggca cggcccacaa
      301 gaacagagat cccgagggcc gagcctgcgt caaccacata agcaactaca ttccaggccc
      361 ccgagcgcgc gacgcgaccg atgaaggatg caggagtagg ctcagcctcg ctcggatgca
      421 ctcgttagag tcacttggcc ctgtcgctta gggctccccg aagcctatgg aaaacgttct
      481 gcgcgatctt ggtggagcgg agaacctgat gcgctagagg gaagttgcgc ttcaagacct
      541 tgcttcgaac ctcccacctt ccggcgaacc ttatcgtgag aacctcatgg ccgcagagca
      601 attgaggggg aaggaaccag tgctcccctt gatctagcat ctcggtaagc atgctgcggt
      661 aggtttcgta ataactcttg ttgaccaagg ccaccttggt ctcgaagcag agaagcggca
      721 caagtttgct gtcgtccttg tagcaatacg ctatgacgcc ctctgtggtt ccggggaagt
      781 cctggttgaa agtgtcatcg acgacagcga cccctccctc cacgagtatc gcttccgcca
      841 actcgagatc ggccttcacc tcgtcggtcg cgtggcctcc gtctaagtag gcaaacctga
      901 agttgctccc gagggcaact gggtcgagat tctgtgatgg gccatgatgg atgacaacat
      961 tcggatcacc cgatttgaat cgttcgaagt tgcgctccca agcggcataa ctcgaaccgg
     1021 ctgacttgga gatgttgaga tcttgctggt cgaaaaggtc ggagacgtgc agggtctctc
     1081 cgtccctcag caggttcgtg aagaagacac tgcttcgagc ctcatgggct ccaacttcga
     1141 ggacgtcgcc cgacatcttg aggttgcgga gctggaaggt gtggaacgca tagaagatct
     1201 gggccgcgtt ggtcgagagc cagccaaggg tacttgggaa atgtttgacc gactcttcga
     1261 actctttcgc aggcaccaag ctgctgatgg tcaatcgatt ccctttcgtt cctcagatcc
     1321 caagctttgg tgcccaacta agggctgctg tcgcccagcc cgtgccctcg attcggcgag
     1381 caacttggaa agcagaaact tcctcagcgg cggcaagccc agccacgata gcgcgagcat
     1441 ccttatcgtc aaaggatatc cgaacagaac tacagcggtg acatacctga ccgcccctgc
     1501 atcgtcacca gacgcgatca ccttgagagc ttcatcgaga gaccatggcc ccccattctc
     1561 ggccaggaat cgtgaccggc gcatatggat cctgccgatg agcgcggccg ggaggaacgt
     1621 ccacaataag gccttgatcc gcacgtcacc gggagccgac aagtccagcc gcagaagcct
     1681 acccaagacc gatcttcggt gacctgccag cagctcagcc ttgaccagat cccactcgaa
     1741 cctggtccta gaagctcggg ctattctgcg cgcagcagga tcgagccccg tctcgagcca
     1801 gcggtcgagg atcatcttcg aatcccgact catgagaagg tgtgacttgc tgagactccc
     1861 cggcgtaacg cgatagtaag cgagcggctc tgggatgaaa gctgctcgct cccccccgac
     1921 aagacatgcg atgttgcgat cccagtcctc gcaccagagc ccttcgtcga acagcccata
     1981 tgtgtcgaag atccgcttcc gatagacgag aaggttggag agatagttgc cacggaagat
     2041 attcacgttc tggtcgtcgg catagaagat cgttccagtg gtgccgaacc aggatcgctg
     2101 cgttcgcact ccatcgatca gtaggtacga atcggaggtc gctatcgagt atgtgtgatc
     2161 gtgttccagg aaaccaacca gaacctccag cctcttgggc atccagacat cgtctgcatc
     2221 cagcagtgcg atatatcgac cctgtgcagc ctggatccca gcattgcggg cagccgccgg
     2281 tcggcgattc tccgggaact cgataacgcg gatcgaat
//
LOCUS       DATDOZ010000290         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2870294, whole genome shotgun sequence.
ACCESSION   DATDOZ010000290 DATDOZ010000000
VERSION     DATDOZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2870294"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VI541_05830"
     CDS             <1..334
                     /locus_tag="VI541_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HLF70460.1"
                     /translation="VVSGVITNAMDAMPSRVRAWIGPCIGPCHYEVGREIQEAYERAH
                     GSQAGVFSQEGTSLRMDLPAAAALQLESSGADVMNSDPLCTYCDARFFSHRRDGGRTG
                     RQAVIVYR"
     gene            331..1008
                     /locus_tag="VI541_05835"
     CDS             331..1008
                     /locus_tag="VI541_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HLF70461.1"
                     /translation="MTIASNLEMVRERVARAAQGVGRAADDVRLVAVSKGQPIASIEE
                     AIAAGQIEFAENRVHELVEKRRDLGSGPTWHFVGQIQRNKVPELVSSADYIHSIDRIE
                     VVREIDERAQIPKMVMIQVGFDGQAGRGGVVPDSLAALLEGTLASERVELVGLMAMAP
                     RGAVPEDARPFFRRLASLRSAMEARFPEAKLQHLSMGMSQDYEVAVEEGATMVRVGEA
                     IFGPRPR"
     gene            1042..1494
                     /gene="sepF"
                     /locus_tag="VI541_05840"
     CDS             1042..1494
                     /gene="sepF"
                     /locus_tag="VI541_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HLF70462.1"
                     /translation="MSSIGVWKKTLVLLGLAEDEDLDDELDLIDETPAENPPSIRKIT
                     REEMSKVHPLPSAQPLGQVHIVAPTSYDDAKEIGDKLRSSVPVIMNLQSVEEDLFKRL
                     TAFASGLAYGLNGEVQRLAPRMYLVTPANVEVSAEDRQRLKRGLFNEF"
     gene            1510..1782
                     /locus_tag="VI541_05845"
     CDS             1510..1782
                     /locus_tag="VI541_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HLF70463.1"
                     /translation="MGLRFILCRILYVYSLILLGRVIVSFLFLFKPDLRPPGPLRVVI
                     EFLYSMTEPPLQLLRRIIPQPAGLPLDLSFLVLFILVQSVLPRIVC"
     gene            1805..2758
                     /locus_tag="VI541_05850"
     CDS             1805..2758
                     /locus_tag="VI541_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HLF70464.1"
                     /translation="MDLTPQDIHEKQFNDAFRGYGHEEVDLFLDQVAESFEKAFAENQ
                     QMHHRMRQLEEELKAALSTEDMLKKTLLAAQKTADEAVDKARAIAQSTVEGAAAKARE
                     ILDKAEATRAEMLSEADHRARELVAGAEIREAEIEDRVAKLKAFDSSYRARLSEFLSS
                     WLSALAEPSADRVSAPPAHQPGPAEVPPVQFRPDVDEIQTPTLAEILGEPSETSEVME
                     PEPVSNLVVEQEVSASLPLEVEAAPEPQAAQPGNDESIEFELSAPEEPEANEIDLTEV
                     NIADLGVFTDDPFTTFSESKVKARSEVDEERAIKELFWGED"
     gene            2866..>3255
                     /locus_tag="VI541_05855"
     CDS             2866..>3255
                     /locus_tag="VI541_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70465.1"
                     /translation="MVTLSVVLVVIATVTLIIGVLSGIDPLIFLSIGSSVVAGILLIK
                     SVLDDRKLRVLTDGPAIDGMATTSALDTTIAEDLSVEDELAEDEGALVGAADDDDDED
                     EEEDLVPMGVPIAAARKPKAVARTAAKP"
BASE COUNT          661 a          944 c         1019 g          631 t
ORIGIN      
        1 cgtcgtgtcc ggagtgatca ccaacgccat ggatgccatg ccttcacgag tgagggcatg
       61 gataggaccc tgcatcggcc cttgccacta cgaggtggga cgcgagatcc aagaggccta
      121 cgagagggct catggctcgc aggccggggt cttcagccag gaggggacgt ctctgcggat
      181 ggaccttcct gccgcggccg ccctccagct cgagtcgtcg ggggccgatg tcatgaactc
      241 cgaccctctc tgcacttact gcgacgcccg cttcttttca caccggcgag atgggggccg
      301 cacaggtcgc caggcggtga tcgtctacag atgaccatcg cgtcgaatct cgagatggtt
      361 cgcgagcggg tcgccagagc cgcgcagggc gtcgggcgtg cagccgacga tgtgcgcctg
      421 gtcgctgttt cgaagggtca gcccatcgca tcgatcgaag aggcgatcgc cgcagggcag
      481 atcgagttcg ctgagaatag agttcatgaa ctcgtcgaga aacgacgcga cctggggtca
      541 ggtccaacct ggcacttcgt tggacagatc caacggaaca aggtcccgga gttggtcagc
      601 agcgccgact acatccactc tatcgatcgc atcgaggttg tcagagagat cgatgagagg
      661 gcgcagatcc ccaagatggt gatgatccag gttggtttcg atgggcaggc cgggcgcggg
      721 ggagtggtgc ccgatagcct cgctgccttg ttagagggta cgcttgcgtc ggagcgtgtt
      781 gagctagttg gtttgatggc gatggctcct cgaggtgcag tgcctgagga tgcccgaccc
      841 ttcttcagac ggctcgccag cttacgcagt gcgatggagg ccaggttccc cgaagcgaag
      901 ctccagcatc tttcgatggg gatgagccaa gattatgagg tcgccgtcga agagggggca
      961 acgatggtgc gagtggggga agctatcttc ggcccccgcc cgcgttgatg cgtttggatg
     1021 ttcgggactt aggaggaact agtgagctcc ataggggttt ggaagaagac tctggtccta
     1081 ctcggcctcg ctgaggacga agaccttgac gacgaactag accttatcga tgagacgccg
     1141 gcagagaacc cgccgagcat ccgcaagatc acgcgagagg agatgtcgaa ggtccatccc
     1201 ctcccttcag cacagccact cggtcaggtg cacatcgtgg cgcctacctc ttacgacgac
     1261 gcgaaggaga tcggcgacaa acttcggtct tccgtgccag tcatcatgaa cctgcagtcc
     1321 gtcgaagagg atctcttcaa gcgtctcacc gccttcgcct cggggctcgc gtatgggctc
     1381 aacggggagg ttcaacggct ggctccccgg atgtatctgg tgacgccagc gaatgtcgag
     1441 gtttccgccg aggatcgcca gcgactcaag cgaggcctgt tcaacgagtt ctagtcatgg
     1501 agtcggttct tgggtttgag attcatcctc tgcaggatcc tgtacgtcta ctcgctgatc
     1561 ctcctcgggc gggtcatcgt ctcgttcctc ttcctattca aacctgatct tcggcccccg
     1621 ggcccccttc gggtggtcat cgaattcctc tactcgatga ccgagccgcc cctacaactc
     1681 ctgcgccgga tcatccccca acccgctggg ctccctttgg acttatcatt cttagtgttg
     1741 ttcatactgg tccagagcgt gctgccgcgg atcgtctgtt aaccgccgca taaaggagac
     1801 tccgttggat ctgacccccc aggacataca tgagaagcag ttcaacgacg ccttccgcgg
     1861 ctacggccac gaagaggtcg atctcttcct cgatcaggtg gcggagtcct tcgagaaagc
     1921 attcgccgag aaccagcaga tgcaccaccg catgaggcag ctcgaagagg aactcaaggc
     1981 ggccctatcc accgaggaca tgctgaaaaa gaccctcctc gccgcccaga agaccgctga
     2041 tgaggcggtc gacaaggcgc gagccatcgc ccagagcacg gtagagggcg ccgcggcgaa
     2101 ggctcgagag atcctcgaca aagccgaggc gaccagggcc gagatgctct ccgaggcgga
     2161 tcatcgagcc cgagaacttg tcgcgggggc cgagatccgt gaggccgaga tcgaggatcg
     2221 tgtcgccaag ctcaaagcgt tcgactcttc atatcgcgcg aggttgtctg agttcctctc
     2281 ctcttggctg agtgcactgg ccgagccttc tgcggaccga gtcagtgctc caccggctca
     2341 tcagccggga ccggcagagg tgccgccggt tcaatttcga ccggacgtgg atgagatcca
     2401 gacgccaacc ctggccgaga tccttggaga gccatctgag acatccgagg tgatggaacc
     2461 cgagcccgtt tccaacttgg tggttgagca ggaagtctct gcgtcgttgc cattggaggt
     2521 ggaggcggct cccgagccgc aggctgcaca gccggggaac gatgagtcca tagagttcga
     2581 gttgtctgcg cccgaagagc ctgaggccaa cgagatcgac ctaaccgagg tcaacatcgc
     2641 tgatctcggg gttttcacag atgacccctt taccacattc tctgagtcaa aggtcaaggc
     2701 ccgctcagaa gttgacgaag agagggcaat caaagaactg ttctggggcg aggactagcc
     2761 cggaaatctc agcggatgtt ggactaacct cgcgcgggcg atagactgcc cgcgattcgg
     2821 tcaggcgagt ccctggccag catcgaagga tatggaggaa catcagtggt aactctgtcg
     2881 gtcgttctgg tcgtgatagc gacggtgact ctcatcatcg gtgtcctatc aggtatcgac
     2941 ccactcatct tcctgtccat aggttcgagc gtggtcgcag ggatcctgtt gatcaagtcg
     3001 gtgctcgatg accgcaagct tcgggttctt accgatgggc ccgccatcga tggcatggca
     3061 accacctccg cgctagacac gaccatcgct gaggatctct cggtcgaaga cgaactcgct
     3121 gaagacgaag gtgcactcgt cggggcggcg gacgacgacg atgacgagga tgaagaagag
     3181 gacctcgtgc cgatgggcgt tccgatcgcg gcggcgagga aaccgaaggc tgtagcaagg
     3241 acagcggcga aacca
//
LOCUS       DATDOZ010000291         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_506072, whole genome shotgun sequence.
ACCESSION   DATDOZ010000291 DATDOZ010000000
VERSION     DATDOZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506072"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1851
                     /gene="groL"
                     /locus_tag="VI541_05860"
     CDS             223..1851
                     /gene="groL"
                     /locus_tag="VI541_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HLF70466.1"
                     /translation="MAKQIAFAEEARRAMEKGMDQLAHAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYERIGAELVKEVAKKTDDVAGDGTTTATVLAHAITKEG
                     LRVVAAGANPIALKRGIERAVDAVVEYIGGQAKEVEGKDEIAHVGAISANNDREIGEI
                     IAEAMDKVGKDAVITVEESNTLGLELELVEGMRFDKGYISPYFITDPERMEAVLEDPY
                     VLVVNSKISAIKDLLPILEKVMQGGKSLLIIAEDVEGEALATLVVNKIRGHFKSVAVK
                     APGFGDRRKAMLQDISILVGGQVISEEVGLKLENTGLELLGSARKVVVTKDETTIVEG
                     SGNADDIKGRINQIRAELEKSDSDYDKEKLQERLAKLAGGVAVIKVGAATEVELKEKK
                     HRIEDAVQATKAAVEEGIVPGGGSTLVFAAASLEKLEIDLTGDELAGVQIVKRSLSAP
                     LKQIANNAGLEGGVIVEKVRTLPKGHGLNAATGEFEDLMKAGIVDPAKVTRSALQNAA
                     SIAALFLTTEAVVADKPEPKPAMPAMPGGGDMDF"
     gene            1921..2148
                     /locus_tag="VI541_05865"
     CDS             1921..2148
                     /locus_tag="VI541_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70467.1"
                     /translation="MSESDQTPDSRIARRNMVVCRSCGATQRVRGDSFPLGWSILGSG
                     SRLQGYLCPECARRQLREIEGRLDLDFELGF"
     gene            complement(2145..>2658)
                     /locus_tag="VI541_05870"
     CDS             complement(2145..>2658)
                     /locus_tag="VI541_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLF70468.1"
                     /translation="WIVNPLYGPLGWIVRAFGGTPGPVLLDGGGALATIAALSAFALG
                     EGFLVMLATRREIPSMLYDAALVEGASRWRLFRGITFPLIAPTMALIAARDLLTSLQN
                     ALVPTILLTHGGPVGATKTLPYLIFERGFLESSPADGAAMAAYLFLGAFVLVAAVLVV
                     RRMSSKDKAA"
BASE COUNT          575 a          775 c          855 g          453 t
ORIGIN      
        1 acttttcttt gtccgggagg gttcgccggg aagggccgat cgcttccgtc gacctatgct
       61 gacgactccg gcgtgagtac ttctggccgg aatcccacgg gttttaggcc cccacttgcg
      121 gattatcccg ttagcactct agattatgga gtgctaacac tcggacaagc cgagtgcgaa
      181 gacagtgtcg ggcatcccgg caggataggt aggaggtagt tgatggctaa gcagatcgct
      241 ttcgcggagg aggctcggcg tgcgatggag aagggtatgg accagttggc ccatgctgtt
      301 cgcgtcacac tcggtcctaa gggccgcaac gtagtactcg agaagaagtg gggagccccc
      361 acgatcacca acgacggtgt ctcgatcgcc aaggagatcg aactcgagga tccgtacgag
      421 aggatcgggg ccgaactggt caaggaagtg gcgaagaaga ccgacgatgt agccggcgac
      481 ggtacaacta ccgctacggt actcgcacac gccatcacca aggaaggcct gcgagtagtt
      541 gcagctggcg ccaacccgat cgccctcaag cgcggtatcg agcgcgcggt cgacgcggtc
      601 gtcgagtaca tcggtggcca ggccaaagag gtcgagggca aggatgagat cgcacacgtc
      661 ggagcaatct cggccaacaa cgaccgcgag atcggcgaga tcatcgccga ggctatggac
      721 aaggtcggca aggacgcagt catcacggtc gaagagagca acactttggg actcgagctc
      781 gagctcgtcg aagggatgcg cttcgacaag ggatacatct cgccatactt catcaccgac
      841 cccgagcgga tggaggcggt actcgaggat ccatacgtcc tcgtagtcaa ctccaagatc
      901 tccgcgatca aagatctgct accgatcctc gagaaggtca tgcagggcgg caagtcgctc
      961 ctgatcatcg ccgaagatgt cgagggcgag gccctcgcta ccctggtcgt caacaagatc
     1021 cgcggtcatt tcaagagtgt cgcggtcaag gcccccgggt tcggagaccg tcgcaaggcc
     1081 atgctccagg acatctccat cctcgtgggt ggacaggtca tctccgagga ggttggcctc
     1141 aagctcgaga acactgggct cgagctgctg ggttctgccc gcaaggtggt ggtcacgaag
     1201 gacgagacca ccatcgttga gggcagtggc aacgccgacg acatcaaggg ccggatcaac
     1261 cagatcagag ccgagctcga gaagagtgat tcggactacg acaaggagaa gctgcaggag
     1321 cgtctggcca agctggcggg cggggttgca gtcatcaagg tcggagcggc caccgaggtc
     1381 gagctgaagg agaagaagca ccgcatcgag gacgccgtcc aggcgaccaa ggccgcggtt
     1441 gaggagggca tcgtcccagg cggcggcagc acgctcgtct tcgcggcggc ttccctcgag
     1501 aagctcgaga tcgatcttac gggcgatgag ctcgccgggg tccagatcgt caagcgctcc
     1561 ctctctgccc cactcaagca gatcgcgaac aacgctggcc tcgaaggcgg ggtcatcgtc
     1621 gagaaggtgc ggacccttcc gaagggccac ggactcaacg ctgccacagg tgagttcgag
     1681 gatctgatga aggctgggat cgtcgacccc gctaaggtca cgcgctcagc cctgcagaat
     1741 gcagcgtcga tcgcagccct gttcctgacg acggaggcag ttgttgccga caagccagaa
     1801 ccgaagccgg ccatgcccgc gatgccgggc ggcggagaca tggacttcta agcaccatca
     1861 ggagtgacgc catgaaaggg gggccggaga cggcccccct tttcgtgtag tttgtcgaac
     1921 gtgagcgagt cagaccagac cccagacagc cggatcgctc gtcgcaacat ggtcgtctgc
     1981 cgctcctgtg gtgcaactca gcgggtgcgt ggggactcct tcccactggg ttggtcgatc
     2041 ctcggcagcg gttcgcgtct ccaggggtat ctctgccctg agtgtgctcg gcgtcagctg
     2101 cgtgagatcg agggccgcct ggatctggac ttcgaactcg gcttctaggc agccttatcc
     2161 ttgctagaca ttcggcgcac caccaggaca gccgctacga ggacgaatgc tcccaagaag
     2221 agatatgcag ccattgcggc gccatccgcg ggtgaagact cgagaaaacc acgctcgaag
     2281 atgaggtagg gaagggtctt ggtcgcaccg acagggcccc catgggtcaa caggatcgtc
     2341 ggcacgaggg cgttctgaag gctggtcaat agatctcttg cagcgatcag cgccatggtc
     2401 ggagctatca agggaaacgt tatccctcgg aagagcctcc acctgctggc tccctcaacg
     2461 agcgctgcgt cgtagagcat gctcgggatc tcgcgacgtg tcgcgagcat tacgaggaac
     2521 ccctcgccaa gtgcgaacgc agagagggca gcaatcgttg caagcgctcc tccgccatca
     2581 agaagcaccg gtccgggagt tccaccgaac gctctgacga tccatccgag ggggccatat
     2641 agcgggttca ctatccac
//
LOCUS       DATDOZ010000292         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_506698, whole genome shotgun sequence.
ACCESSION   DATDOZ010000292 DATDOZ010000000
VERSION     DATDOZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506698"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="purM"
                     /locus_tag="VI541_05875"
     CDS             <1..624
                     /gene="purM"
                     /locus_tag="VI541_05875"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HLF70469.1"
                     /translation="TAEHPGHMTPNSYDLAGFCVGVVERSRIVTGSSIQVGDVVLGIE
                     SSGLHSNGYSLVRKILLSDMKLDLEDRPIELGHTLGEELLRPTVIYSSVVGALMEEVD
                     VHGLSHITGGGLPENVGRIMPEGLSAHIAKRSWEPHPIFGLISSLGQVEEREMFSTFN
                     MGIGMVVVVQPEASNHALDVIRSRGHRAHEIGEVLTRSKDHGPIRLG"
     gene            621..>2024
                     /locus_tag="VI541_05880"
     CDS             621..>2024
                     /locus_tag="VI541_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF70470.1"
                     /translation="MRTTAERPRTTTSPQRRIAGLRDFKTPTLEAVERRRWQLWLLAG
                     FVIFGLALSTTLLTVDNTVFTSLSFLPIYVIRVLFMGIAAAIGLYLVDKEYRLRKLTH
                     DLVDERVLSAALTNRLKELSVLSEVGKAINQVLDIDDVLRMILRSAVEMLEADRGSVM
                     LISEDGEELVLAHAQGFDGLDVEGSVVPLGVGIAGQVARDREPILVNGSAPTDLLTDD
                     RHSEAVSSISVPFVSKDELHGVLNVSVVTHLREFSEYDLRAIGLFAEHAAIAIRNARA
                     YEQEKQAIFRFEQADKLKSEFLATVSHELRTPLTSIIGCATTIRKRPHELSEAHKAEF
                     LELIEQQAGRLLHMVEDLLSASMIESGGSALTRKPVDLVALALSIERAFEAAGSVGPI
                     KVDAPERVIAYGDAMALEQVLTNLMDNAAKYSDRGSPIVLRVWEEPGDACFSVTDRGR
                     GIAEDEIGQVFERFKQLD"
BASE COUNT          427 a          569 c          615 g          413 t
ORIGIN      
        1 acagctgaac atcccggcca catgacaccg aactcttacg atctagctgg gttctgtgtg
       61 ggtgtggtcg agaggtctcg catcgtgaca ggatcttcga tccaggtcgg cgatgtcgta
      121 ctcggcatcg aatcctcagg gctccattcg aacgggtact cgttggtcag aaagatcctg
      181 ctgtccgaca tgaagctcga cctagaagat cgacccatcg aactcggaca cacgctcggt
      241 gaggagctgt tgaggcccac ggtcatctac tcgagcgtgg ttggggccct gatggaagag
      301 gttgatgtcc acggcctgag ccacatcaca ggtgggggtc tccccgagaa cgtgggacgg
      361 atcatgcccg aaggtctctc ggctcacatc gcgaagcgct cctgggaacc gcacccgata
      421 ttcggcctaa tctcttcgct cggtcaggtc gaagaaagag agatgttctc aaccttcaac
      481 atgggcatag gaatggtcgt cgtggtgcag ccggaagcct cgaaccatgc gctcgatgtg
      541 atccgctcgc gggggcatag ggctcatgag atcggcgagg ttcttacgcg cagcaaggat
      601 cacggaccca tccgactggg atgagaacta cggctgaacg tccgcgaacg acgacgagcc
      661 cccagaggag gatcgcgggc cttagagact tcaagacccc cacactcgag gcagtcgagc
      721 gtcgcaggtg gcaactctgg ctacttgcag ggttcgtcat cttcgggttg gccttatcca
      781 cgacgctgct gacagttgac aacaccgtct tcacgtcgct cagcttccta cccatctatg
      841 tcatacgcgt tctattcatg ggcatagcag ctgccatagg tctgtatctg gtcgacaagg
      901 agtatcgact tcgcaagctc actcacgatc tggtcgacga gagggttctg tcggccgcct
      961 tgaccaatcg cctcaaggag ctatcggtgt tgtctgaggt gggcaaggcg atcaaccagg
     1021 tcctcgacat cgacgacgtc ttgaggatga tcctccgatc ggcggtcgag atgctcgaag
     1081 ccgatcgcgg atcggtcatg ctgatctcgg aagacggaga ggaactggtg ttggctcacg
     1141 ctcaaggatt cgatggactg gatgtcgaag gttccgtcgt gccgctcgga gtggggatcg
     1201 caggtcaggt cgctagggat cgtgagccga ttcttgtcaa tgggtcagct ccgaccgatc
     1261 tgctcacgga cgacagacac agcgaagcgg tctcatcgat atccgttcca ttcgtgagca
     1321 aggatgaact ccatggtgtg ctcaacgtga gtgtggtaac gcatctgcgc gagttcagtg
     1381 agtatgacct aagggcgatc gggctcttcg ccgagcacgc ggcgatagct atccgcaacg
     1441 caagggctta tgagcaagag aagcaggcga tcttccgctt cgagcaggca gacaagctca
     1501 agagcgagtt tctagcgacg gtctcacatg aactgcggac ccccctcaca tccatcatcg
     1561 ggtgcgccac cacgatccgc aagagacctc atgagttatc cgaggcccac aaggccgagt
     1621 tcctcgaact gatcgaacag caggctgggc gcctgctgca catggtcgag gatctgctct
     1681 cggcttcgat gatcgagtcc ggaggttcag cactgacccg caagccggtc gatctggtcg
     1741 ctctcgctct gtccatcgaa cgcgccttcg aagctgcggg gtcggttggc ccgatcaagg
     1801 tcgacgcccc cgagagggtg atcgcatatg gcgatgccat ggctctcgaa caggtactga
     1861 ccaatctgat ggataacgcg gcgaagtact cggaccgggg ctcccccatc gtcctgcgag
     1921 tctgggagga acctggcgac gcgtgcttct ctgtcacgga ccgtggaaga gggatcgccg
     1981 aggatgagat cggtcaggtt ttcgaaaggt tcaaacaact cgat
//
LOCUS       DATDOZ010000293         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_247819, whole genome shotgun sequence.
ACCESSION   DATDOZ010000293 DATDOZ010000000
VERSION     DATDOZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247819"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VI541_05885"
     CDS             <1..431
                     /locus_tag="VI541_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70471.1"
                     /translation="GDAAGMVNPFNGEGIAYALETGMLAAGVVADALQNGRNTELVAY
                     REALHDTYGAYYRIGRKFTKIIGRPKIFRALCLVGMRSQTVMEFVFQVLANLGEAQGG
                     GLTDKTLRAMCRLAEQDLSELKEPEISAPTPAVSRKAGAA"
     gene            428..790
                     /gene="ndhC"
                     /locus_tag="VI541_05890"
     CDS             428..790
                     /gene="ndhC"
                     /locus_tag="VI541_05890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLF70472.1"
                     /translation="MTLSAYLPVLILGVVTLLFGLGSVMASSLLSPKYPTPAKLAPYE
                     CGITPTGIEGGDRFPIKFYVFAMLFIIFDIETVFMYPWAVVFKRIGIAGIVEMGFFAL
                     FLLGAYVYVWKSGGLEWE"
     gene            781..1236
                     /locus_tag="VI541_05895"
                     /pseudo
     CDS             781..1236
                     /locus_tag="VI541_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            1263..1736
                     /locus_tag="VI541_05900"
     CDS             1263..1736
                     /locus_tag="VI541_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLF70473.1"
                     /translation="MNATAVRIAEKFPDAFADVSATGNFLTVAAEKWMTVARWLAGEG
                     GFSLLSNLCGADRGGAERFEVMYNLTNMQAREVLGIRLKIPDGDPPKAPSVTSIWPGA
                     NFPEREAYDMFGIVFDGHPDLNRILMPDEWEGHPLRKDYATGKVPIEFKHMSPGF"
     gene            1808..2391
                     /locus_tag="VI541_05905"
                     /pseudo
     CDS             1808..2391
                     /locus_tag="VI541_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
BASE COUNT          464 a          695 c          735 g          497 t
ORIGIN      
        1 tgggtgatgc cgcagggatg gtcaacccct tcaatggcga ggggatcgcc tatgcgctcg
       61 agacaggcat gcttgccgct ggggttgttg cggatgcgct ccaaaacggt cgcaacaccg
      121 aacttgtggc gtaccgcgaa gctcttcatg acacttatgg cgcctactac cggatcgggc
      181 gtaagttcac gaagatcatc ggtaggccca agatcttcag ggctctctgc cttgtcggta
      241 tgcggtcgca gaccgtgatg gagttcgtct tccaggtgtt ggccaaccta ggtgaagcgc
      301 agggtggcgg cttgaccgac aagaccttgc gcgccatgtg cagactcgct gagcaggatc
      361 tgtccgagtt gaaggaacct gagatttcgg cccccacacc ggcggtgagc aggaaggcag
      421 gcgcggcatg acgctctcgg cctatctgcc ggttctgata ctcggcgtcg tgaccttgct
      481 cttcggcctc ggctcggtca tggcctcatc cttgctctcg ccaaagtacc cgacaccagc
      541 gaagttggcg ccgtacgagt gcggtatcac cccgactggc atcgagggcg gtgatcgctt
      601 ccccatcaag ttctatgtct tcgcgatgct gttcatcatc ttcgacatag agaccgtctt
      661 catgtatccg tgggcagtcg tgttcaagcg catcggtatc gctggcatcg tcgagatggg
      721 cttcttcgcc ctgttcctgc tgggcgccta tgtctatgtt tggaagagcg ggggcctgga
      781 gtgggagtga ccagcgagaa gggcggcatc ctcctggggc aactcgacag ggtcgcagat
      841 tgggcccgca ggtcgagtct gtggcccgcc acattcggat tggcctgctg cgctatcgag
      901 atgatggcgt cgggggcaag tcgcttcgac ctatccaggt tcggcatgga ggtcttccga
      961 gcgtcacctc gtcaggcaga cctcatgatc gtggccggac gtgtctcaca gaagatggcc
     1021 cccgttctgc gacagatcta cgaccagatg ccggaaccca aatgggtgat ctcgatgggg
     1081 gcatgtgcgt cctgcggtgg cgtcttcaac aactacgcgc tcgtgcaggg ggtcgaccag
     1141 atcgttccgg ttgacatata cgtccctggc tgtccgccgc gccccgagat gctcatcgac
     1201 gggatcatga aactgcacga caagatcctt tcgggagagt accgacgtga acaggtgcct
     1261 cgatgaacgc cacggcagtt cgcatcgctg agaagtttcc cgatgccttc gccgatgtat
     1321 ccgcgaccgg taacttcctg accgtcgctg cggagaagtg gatgacggtt gcccgctggc
     1381 tcgctgggga gggtggcttc agcctcctct cgaatctctg tggcgccgac cgcggggggg
     1441 ccgaaagatt cgaggtcatg tacaacctga cgaacatgca ggcgcgcgag gtcctcggta
     1501 tccggttgaa gatccccgac ggcgacccgc cgaaggctcc ctcggtcaca tccatctggc
     1561 ccggtgccaa cttccccgaa cgcgaggcct acgacatgtt tggcatcgtt ttcgacggtc
     1621 atccggacct gaaccgcatc ttgatgccag acgagtggga gggtcatcca ctccgtaagg
     1681 actatgcgac ggggaaggtt cccatcgagt tcaagcacat gtcgccgggg ttctgatgtc
     1741 gaaacgagaa gcagttgaga tattcgaagc cgcacccggc ggcgcgtacg tcgaagagtg
     1801 ggatgagtcc tcggaactca tgaccatcaa catgggaccc cagcatcctt caacgcatgg
     1861 ggtgttgaga ctggtgctga cgctcgaagg cgagacggtt cgcgacgtga aaccggttat
     1921 cggctatctc cacaccggta tggagaagga gtgcgaagac aacaactggc gtcaagccgt
     1981 caccatcgtc acgcggatgg actatctcgc cccactccac aacgaactcg tctactcatt
     2041 ggcggtggag catcttctgg ggctcgaggg tccggaacgt gctcaggcga tcagggtgct
     2101 catgaccgag ctgaatcgga tcacttcaca cctcgtctgg ctctcaacac agggcatgga
     2161 catgggtgcg atatcggtga tgctctacgg gttccgagag cgcgagctga tcatcgactt
     2221 cttcgagatg gtcaccggac ttcgcatgaa ccacaactac atctgtccag gtggcgtttg
     2281 gcaggacctt cccgaaggct ggcagactcc gatccgcgac ttcatcgaga tattccccgg
     2341 tcggctgaag gagtacgaga cccttctcac agacaacccg atctggcgac a
//
LOCUS       DATDOZ010000294         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1820421, whole genome shotgun sequence.
ACCESSION   DATDOZ010000294 DATDOZ010000000
VERSION     DATDOZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820421"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..422)
                     /locus_tag="VI541_05910"
     CDS             complement(117..422)
                     /locus_tag="VI541_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HLF70474.1"
                     /translation="MDMNKMMKQVQKMQADLAKAQEDLAEQEIEGQAGNGLVKVTVTG
                     GGEFTAIAIDPKAVDPDDSSLLEDLVLAAVRDALSRQQALAQDRLAPLTGGLGLPGL"
     gene            complement(425..2161)
                     /gene="dnaX"
                     /locus_tag="VI541_05915"
     CDS             complement(425..2161)
                     /gene="dnaX"
                     /locus_tag="VI541_05915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLF70475.1"
                     /translation="MLTTSMAHLSLYRKYRPRSFADVIGQSHVTQTLLNALTEGRISH
                     AFLFSGPRGTGKTSTARILAKALNCEKGGPEPCNECRSCIEIGDGISLDVVEIDAASH
                     GSVDDARDIREKVAYAPVGGRWRVYIIDECHMLSPAANNAMLKVLEEPPSHVVFVFAT
                     TEPNKVLQTLLDRCQSYGFRAIGDADIAECVIRVGEAEGLSIEDDAVALIATRAAGSA
                     RDALSILDQLTSFAGNKITFEDVGSMFGSLPDELLFEAVDIICEHDAGSGFLFTDRLI
                     RSGADLREFVRALIDHLRAIFLIHHASAAQEILDAPDDKLERLRAQANRLDPAEVLRL
                     IDLAAEVNVQMRQALDARIALEVGLARMARPDLRPNAPGPDRSDGAGVASDAGARPAR
                     RLAPGSSGPDESAKLTTPAARSPKSTSLKSEDPSSSEAEPDVATDIVIEPGEVDLERV
                     LRAWPVILEKVKKRKISFQALLLPATPVAWGDGELILGFGPRSRFHRDRVGEVGQNLP
                     LIEAFAQVLGVTPRIRCVIVEEEETRASEPESVDASPDLSGSTEAEPVEAKSSLDLIR
                     QAFGDAEVVEEK"
     gene            2212..3285
                     /locus_tag="VI541_05920"
     CDS             2212..3285
                     /locus_tag="VI541_05920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLF70476.1"
                     /translation="MRIWEEEVRGGYPPMYSLTLSGLERMLAFTQGRAPRPPVHHLTG
                     LTPVEASPGTSTFTMPSTGWLNSPTGIFQPGVLAFLADAPLGTALMTGLPAGEVTTTS
                     DLTMSYLRPAGIESGLLTGKAKTIHLGRNLGLAEITITDEGGKVLAHGTTRCFILRFP
                     VPDGLEPPQEDLPSYEDDPYQRDVVGDVLPQEVWNSLTGLEIMLGFINGELPPPPIHY
                     LTGMHPTSASEGEAEFVIPATRWLTSPASTIYGGSIALLADAALSSAVQTTVPAATAF
                     APLDLKVNYLRPAFADDKDLVARAHVTHRGKRLAVAEAELTNAEGKTVAIATGSSLIM
                     PGRSWSGSPLVAADEATAEDTGQ"
     gene            3407..3886
                     /locus_tag="VI541_05925"
     CDS             3407..3886
                     /locus_tag="VI541_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70477.1"
                     /translation="MEQSDQLAALIDTGRTLRAANIPFALIGGIAVGIRSGIPRATDD
                     IDVAVATTAARSAVTQVLEAVGFTFQREFEHSVNYRHPNGEPVQAAFDPLFDEMIGRA
                     EPIAILGEEIPVVLTEDLIEMKMRASRSPARRKSKSLRDQADAELLRGDVPGPDEGW"
BASE COUNT          731 a         1283 c         1206 g          703 t
ORIGIN      
        1 ggcgttgcgc ggtcttcacc ccgataccag gcagacgcga aagttcgtcg acaagatctt
       61 gaacgggcgg tgcgaacatg gggagtgcta cctcgcggag tcggagagtc gctcgatcat
      121 agtccgggga gtccgaggcc ccccgtcaat ggcgcgagcc gatcctgcgc aagcgcctgc
      181 tgacgtgaga gcgcatcgcg gaccgccgcg agaacgaggt cctcgagcag actcgagtca
      241 tcggggtcga cggcctttgg gtctatcgcg atcgcggtga actcccctcc gccagtgacc
      301 gtcaccttca ccagtccgtt ccccgcctgg ccctctatct cctgctccgc aaggtcctcc
      361 tgagctttcg ccaggtcagc ctgcatcttc tggacctgct tcatcatctt gttcatatcc
      421 atcgctactt ctcctcgacc acttcggcgt cgccgaaggc ctgcctgatc agatcgagac
      481 tcgacttcgc ctcgacgggt tccgcctcgg tcgaaccgga caggtccggc gatgcatcga
      541 ccgactcggg ctcgctcgcc cttgtctctt cctcttcgac gatgacgcag cggatccgcg
      601 gcgtcacccc aagcacctgc gcgaacgcct cgatcagtgg aaggttctga ccgacctcgc
      661 caacacggtc acgatggaac cgcgatcggg ggccgaaccc caagatcagt tcgccatccc
      721 cccaagccac cggcgtcgca ggcaacaaca gggcctggaa cgagatcttc cgcttcttga
      781 ccttctccaa tatgactggc cacgcccgca gcaccctctc aagatccacc tcgccaggtt
      841 cgatgacgat gtccgtagcg acgtcaggct cggcctccga cgatgaagga tcctcgctct
      901 tcagactggt gctcttagga ctcctggcgg caggggtggt cagtttggcc gactcgtccg
      961 gcccagaaga cccaggcgcg agccgccgcg ccggacgagc gccagcgtcg gaggccacac
     1021 cggccccatc ggagcggtca ggcccgggcg cattcgggcg gagatcgggg cgggccatgc
     1081 gggcgagtcc gacctctaag gcgatgcgcg cgtcgagtgc ttggcgcatc tggacgttca
     1141 cttcggcggc caggtcgatg aggcgcagga cttcggctgg gtcgagacgg ttcgcctgcg
     1201 cgcgcagacg ctccagcttg tcgtcggggg catcgaggat ctcctgcgcg gcacttgcgt
     1261 ggtgtatcag gaagatggcg cgtaggtggt cgatcaatgc gcgcacgaac tctcgaaggt
     1321 cggcccccga tcggatgagt cggtccgtga acaggaagcc cgaccccgcg tcgtgctcgc
     1381 agatgatgtc gacagcctcg aagagaagtt catcgggcag tgacccgaac atcgagccga
     1441 catcttcgaa ggtgatcttg ttccctgcga acgaggtgag ttgatcgagg atgctcagag
     1501 cgtcgcgagc agatccagcc gcgcgcgtcg cgatcaacgc aaccgcatcg tcttctatcg
     1561 acaacccttc ggcctcgccg acgcggatca cacactcagc gatatcagca tcgccgatgg
     1621 cgcggaaccc gtaggactga cacctgtcga ggagtgtctg gagaaccttg ttgggctcgg
     1681 tcgtggcgaa gacgaagaca acgtgtgatg gaggctcctc gaggaccttg agcatcgcgt
     1741 tgttggccgc gggcgagagc atgtggcact cgtcgatgat gtagaccctc cagcggcccc
     1801 cgaccggcgc gtacgcgacc ttctcgcgga tgtcacgggc atcgtcgaca gaaccatgag
     1861 atgccgcgtc gatctcgacg acgtcgagcg agatgccgtc gccgatctcg atgcagctgc
     1921 ggcactcatt gcatggctca gggcccccct tctcgcagtt gagggccttg gcgaggatgc
     1981 gagccgttga tgtcttgccc gtgccgcgcg gcccgctgaa caggaacgcg tgagagatgc
     2041 gcccctcggt cagagcgttg agcagggtct gagtcacatg gctctgcccg atgacgtcgg
     2101 cgaaggagcg gggcctgtac ttcctataaa gggataggtg cgccatagaa gttgtcagca
     2161 tatctcgccc ccctgacgcg ctaacctggc ccagcggata gattcgcgtt catgcggatc
     2221 tgggaggaag aggtgcgggg cggctacccg ccgatgtact ccctgaccct ctctggcctc
     2281 gaacggatgc tcgcattcac gcagggccgg gcccccaggc cccccgtcca ccacttgacc
     2341 gggctgaccc ccgtcgaagc gagccccggc acatcgacat tcacgatgcc ttcgaccgga
     2401 tggctcaact caccgaccgg catattccaa cctggggtcc tcgcgttcct cgccgatgcg
     2461 cccctcggga ccgcgctcat gacggggctc cccgcggggg aggtgacaac cacatccgac
     2521 ctgaccatga gctaccttcg gcccgcgggc atcgagtcgg gccttctcac cgggaaggcc
     2581 aagaccatcc atctaggtcg caatctcggg ctcgcagaga tcacgatcac cgacgagggg
     2641 ggcaaggtcc tcgcgcatgg gacgacccgc tgcttcatcc tcaggttccc cgtaccggac
     2701 ggcctcgaac caccccagga ggatctccct tcttatgagg acgacccata tcaaagggat
     2761 gtggtcggag acgttctccc gcaggaggtt tggaactcgc tgacagggct cgagatcatg
     2821 ttgggtttca tcaacggaga actgccgccg ccgccgatcc actacctaac cgggatgcac
     2881 ccgacgtcgg cctctgaagg agaggccgag ttcgtgatcc cggcgacacg ttggctcacg
     2941 agcccagcga gcaccatcta tgggggttcg atcgcgctcc tagccgatgc tgctctctcg
     3001 tcggccgtgc aaacaaccgt tcctgctgcg accgcgttcg cgccgttgga tctgaaggtg
     3061 aactacctgc gacctgcttt cgccgatgac aaggacctcg tcgctcgtgc ccatgtgacg
     3121 caccgcggaa agaggctggc tgttgcggaa gccgagctca ccaatgccga gggcaaaaca
     3181 gtcgcgatcg ccaccggctc ctctctcatc atgccgggcc gcagctggag cggctcgccg
     3241 ctagtggccg ccgacgaggc tacggccgag gacaccggtc aatagaaggc ccatgcgttg
     3301 gtatcctcgt tgagtgagca tccggtcaga cagtacggtg atagagcaga tcgctcgagc
     3361 gacgaaacgg gagcgtctgc tcatcgccgc gttcaaagga caagccatgg aacaaagtga
     3421 ccaactggcg gcattgatcg acacaggccg gacgctgcgc gcggccaaca tcccattcgc
     3481 tctcatcggg ggcatcgcag tcggcatccg ctcaggcatc cctcgtgcca ccgacgacat
     3541 cgatgtcgcc gtcgctacga cggctgctag atcggcggta actcaagtcc tcgaggcggt
     3601 cggattcaca tttcaacgag agtttgagca cagcgtgaac taccgtcatc caaacgggga
     3661 gcctgttcag gcagctttcg atccactctt cgacgagatg atcggacggg ccgagcccat
     3721 cgcgatcttg ggggaggaga tccctgtagt cctcacggaa gatctcatcg agatgaagat
     3781 gagggcatca cgttccccgg cgcgccgtaa gagcaagtca ctgcgcgatc aggcagatgc
     3841 cgaacttcta cgcggtgacg tgcccggccc cgacgagggc tggtagcccc aaaccctcac
     3901 tcggtctgtt cgtagaaccg gtc
//
LOCUS       DATDOZ010000295         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_508206, whole genome shotgun sequence.
ACCESSION   DATDOZ010000295 DATDOZ010000000
VERSION     DATDOZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508206"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VI541_05930"
     CDS             <1..337
                     /locus_tag="VI541_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HLF70478.1"
                     /translation="RERMAKYRQHRSDTQPVDAPNAGSMFRNPEGYAAGALIESAGLK
                     GYTVGGAEVSGKHANFFLAHPGTRAQDVYDLMAHVQGVVQTTHDVLLIPEVKLVGEFD
                     TTSGLRLAP"
     gene            400..1044
                     /locus_tag="VI541_05935"
     CDS             400..1044
                     /locus_tag="VI541_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HLF70479.1"
                     /translation="MWGSPALGLRRIQVLGNHRVDASTIARSTDLVRGARLVDLSTSA
                     VELKVERIAWVEHARVERIVPSTVRITVTERKVAATVMIGVTRFLVDATGVILQEGEE
                     GKLLITDLRLPKVTLGQRVELITFRDSLRIAAALPGDIKAALLSVSAPSVDGITLRLN
                     DGVEVRYGAAEEMRDKNYAIEKVLENERSRGRQVAYLDVRDPQRPSVMPRSPSG"
     gene            1174..2280
                     /gene="ftsZ"
                     /locus_tag="VI541_05940"
     CDS             1174..2280
                     /gene="ftsZ"
                     /locus_tag="VI541_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLF70480.1"
                     /translation="MATAPQNYLAVIKVVGIGGGGVNAVNRMIDVGLKGVEFIAVNTD
                     AQALLMSDADVKLDIGRDLTRGLGAGADPELGRQAAEAHRDEIEEVLKGADMVFVTAG
                     KGGGTGTGGAPVIAEIAKSLGALTIGVVTRPFGFEGRRRSVNAEQGILTLKDRLDSLI
                     IIPNDRLLQVCDETTSVLEAFRMADEVLLQGVQGITDLITTPGLINLDFNDVKAIMTN
                     AGSSLMGIGSARGENRAASAARNAVSSPLLEASIDGARGVLISISGGSDLGLFEVHEA
                     ADIIFKSAHPDANIIFGAVIDDQLGDEVKVTVIASGFERAERVAAPSFISSSDDDEYE
                     DDDELDRGSPTVTHVFDAAAEEEDLDIPSFLRKS"
BASE COUNT          466 a          644 c          751 g          465 t
ORIGIN      
        1 ccgggaaagg atggcgaagt accgccagca tcgatccgat acccagccgg tggacgctcc
       61 taacgcgggg tccatgttcc gcaaccctga agggtatgca gcaggcgccc tcatcgagtc
      121 tgcggggctg aagggctaca cggttggagg tgcagaggta tcgggcaagc atgcgaactt
      181 cttcctggcg cacccgggaa ccagagccca ggatgtctac gatctgatgg cccacgttca
      241 gggtgtggtt cagacgacgc acgacgttct gttgatacca gaggtcaaac tggttggaga
      301 gttcgacacc acatcggggc tgaggctcgc cccgtgaggt tcgttcgtta cctgttagtg
      361 atggcgctcc tcgttggggc cgtttggggg ggacggctgg tgtgggggtc gcccgccttg
      421 ggtctacgaa ggatccaggt gctggggaac caccgcgtcg acgcctcgac gatcgcgcgc
      481 tcgacggatc ttgttagagg cgcacgacta gtcgatctat cgacatccgc tgtagagcta
      541 aaggtcgaga ggatcgcttg ggtcgagcac gcccgcgtcg agaggatcgt gccctcgacc
      601 gttcgcatca ctgtcaccga acgcaaggtc gcggccacgg tgatgatcgg tgtgacgcgt
      661 ttcctggtcg atgcgaccgg tgtgatcctg caagaggggg aggagggcaa actcctgatc
      721 accgatcttc gactgccgaa agtgactctc ggtcaacggg ttgaactgat cacattcaga
      781 gactcactgc gtatcgcagc ggcgttgcca ggggatatca aggcggccct gttgtcggtg
      841 agtgccccct cggtagacgg gatcactctt cggctgaatg acggggtcga ggtccgttat
      901 ggggccgcag aggagatgcg agacaagaac tatgccatcg aaaaagttct cgagaacgag
      961 agaagtcgcg gcaggcaggt ggcctacctc gatgtccgcg acccacagcg accttcagtg
     1021 atgccgcgga gcccctcggg ctgaggagaa ttattggggg ttgaccgtcc gggaactccc
     1081 ccctatattg ctcaggtaaa tgttgaggtt gaccttgtat caacctatgg tcgagagtca
     1141 cactcaacgg tcctcaaccc ccggaggtgc aagttggcta ctgctccaca gaattacctc
     1201 gcagttatca aggtcgtcgg tatcggcggc ggcggtgtta acgcggtgaa ccggatgatc
     1261 gacgtcggtc tgaagggcgt cgagttcatc gcagtgaaca ccgatgctca agcacttctc
     1321 atgtccgacg cggatgtgaa gttggacatc ggtcgcgacc tcactcgcgg tctcggtgct
     1381 ggtgccgatc ccgaactggg ccggcaagcc gcagaagctc accgcgacga gatcgaggag
     1441 gtgctcaaag gcgccgacat ggttttcgtt acggctggta agggtggcgg caccggtacc
     1501 ggtggtgctc ccgtgatcgc agagatcgca aagagtctcg gtgctctgac gatcggtgtt
     1561 gtaacgcgac ctttcggttt cgaaggtcgt cgtagaagtg tgaatgccga gcaaggcatc
     1621 cttactctca aggaccgact cgacagtctg atcatcatcc cgaacgatcg actgctccag
     1681 gtgtgtgacg agacgacttc agtgctcgaa gcattccgca tggccgacga ggttctgctt
     1741 cagggtgttc aggggatcac cgacctgatc acgacgcccg gcctcatcaa tctcgacttc
     1801 aacgacgtaa aggcgatcat gactaatgcc ggttcgtcgt tgatgggtat cggttcagcg
     1861 cgcggcgaaa accgggcggc gagtgcggca aggaatgcag tctcatcgcc actcctcgaa
     1921 gcctctatcg acggcgccag gggcgttctg atcagcatct ctggtgggag cgacctgggc
     1981 ctcttcgagg ttcacgaggc cgccgacatc atcttcaagt cggcacaccc agatgcgaac
     2041 atcatcttcg gagcggtcat cgacgaccag ctgggagatg aggtcaaggt cacggtcatc
     2101 gcatcgggct tcgagcgtgc cgagcgagtg gcggccccaa gcttcatctc gtcgtcagat
     2161 gacgacgagt acgaggatga cgacgaactg gatcgcggct ccccgactgt tacgcatgtc
     2221 ttcgatgcag cggccgagga agaggatctg gacatcccaa gctttcttcg caagtcgtag
     2281 cggagcgtga tgtggtctta cgcagcgggg cccccgatcg ggggcc
//
LOCUS       DATDOZ010000296         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1822094, whole genome shotgun sequence.
ACCESSION   DATDOZ010000296 DATDOZ010000000
VERSION     DATDOZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822094"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VI541_05945"
     CDS             complement(<1..722)
                     /locus_tag="VI541_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HLF70481.1"
                     /translation="MAITKELASRHGRVRSALLEGEFDALIAYSTAKVAANVRYLTDY
                     FVRFAGMQNIREGEYHMFGSCAVLFPADGAPMVRTDQPWDIARAKEISVFPDTAYTGL
                     FAQDFAPVIRERGYRRVAIDNWYIFPAREFMALTEMAPEVEFVPSHLMSEVRRIKSEE
                     ELEIMRRSAALAVVAVETALDAVQVGANEYDVVLLCEETMRRGGDIELAGGSISGCGP
                     NTSTGSWVPSRTQSRAIEAGEWV"
     gene            839..2170
                     /locus_tag="VI541_05950"
     CDS             839..2170
                     /locus_tag="VI541_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF70482.1"
                     /translation="MATKRGLLVVFLTIGLVTTACAGGDSEGGSTTVGSTTVGSTTGG
                     EDLPTLKVGVPLPFTGVVAESAEEMRQTFLMYVEEQGGTLGGLPVEVIFEDTETDAEL
                     VVTKTRKLIDEDQVHLISGPMLAFEGLAMLDSVNQAGIPWVGQTGVAADDYTRLETPT
                     LSAAAKHTASQETMPLGTYAYEDLGYRTIATLGQDYAWGYQTVGGFQYAFEAAGGDVV
                     QKLWAPIGTTDFAPFVNDINQDVDAVYVTLIGADIPRFVQAYSDFGLKENIPMLASED
                     LVAQDAIRFYSGDAAIGIIGITPFTANLDRPEMQAFVEAYSERTGNIPTFWGESSYVA
                     AMVIDRTLQYVSEANDIALADLPEWVRSNGEAFAAATREIDLSDAPSSAITVDEFNRQ
                     VRDFYIVELVDEGGTITDSLVETVPEVTQFWTFDQQEFLADPLFSREDPPA"
BASE COUNT          434 a          696 c          688 g          406 t
ORIGIN      
        1 acccactcac cggcctcgat cgctctactc tgcgtgcgcg agggaaccca gctcccagtg
       61 gatgtgttcg gtccgcatcc ggaaatcgat ccgcctgcga gctcgatgtc acccccccgc
      121 cgcatcgtct cttcacagag aagcacgacg tcgtactcgt tcgccccgac ctgcaccgca
      181 tcgagcgccg tctcgacggc cacgacggcg agcgcagccg acctccgcat gatctcgagc
      241 tcctcttcgg atttgatgcg acgcacctcg gacatcagat gtgaagggac aaactcgacc
      301 tcgggggcca tctcggtcag cgccatgaac tcgcgtgccg ggaagatgta ccaattgtcg
      361 atcgccactc gccggtatcc gcgctcgcgg atcacggggg cgaaatcctg ggcgaagagc
      421 cctgtgtagg cagtatcagg gaatacggag atctccttcg ccctcgcgat atcccagggc
      481 tgatccgtgc gaaccatcgg cgcgccgtca gccgggaaga ggacggcgca cgagccgaac
      541 atgtggtact cgccctcgcg gatgttctgc atccctgcga accggacgaa gtagtccgtg
      601 aggtatcgca cgttcgccgc gaccttggcg gtggagtagg cgatcagtgc gtcgaactca
      661 ccctcgagga gcgccgacct gacacgacca tgccgactcg ccaactcctt cgtgatcgcc
      721 atgaggtgct cctttactcc gcgggcgtcc gtctacggct cacagcccgc gggaccggct
      781 ggatgctata ttctacagga tgcaggggtc atcatacccg ctctccaaag gaggcatcat
      841 ggcgacgaag cgcggattgt tggtggtctt tctcacgatc ggattggtaa caacagcatg
      901 cgctgggggt gactctgaag ggggctcaac aacggtgggc tcaacaacgg tcggctcgac
      961 aaccggtggt gaggacctac ccaccctcaa ggttggcgtc cccttgcctt tcaccggggt
     1021 cgtggcggag tcagcagaag agatgcggca gaccttcctt atgtacgtgg aggagcaagg
     1081 cggcacgctc gggggactcc cggtcgaggt gatcttcgag gacaccgaga cggatgctga
     1141 gctggtcgtc acgaagacca ggaagctcat cgacgaggat caggttcacc tgatttccgg
     1201 cccgatgttg gcattcgagg gcctggcgat gctcgactct gtcaaccagg ctgggatccc
     1261 gtgggtcggg cagacggggg ttgcggccga tgactacacg cgtctggaga ccccgacgtt
     1321 gagcgcggcc gccaagcaca cggccagtca ggagacgatg ccgctcggga cctacgccta
     1381 cgaggatttg gggtatcgaa cgatcgcgac cctcgggcag gattacgctt ggggttatca
     1441 aacggtcgga gggttccagt acgccttcga agccgctggg ggcgacgtcg tccagaagct
     1501 ctgggcaccg atcggaacga ccgactttgc gccgttcgtc aacgatatca atcaggatgt
     1561 cgacgccgtc tacgtgacgt tgatcggggc agatatccca cgattcgtgc aggcgtactc
     1621 tgacttcggc ctgaaggaga acatcccgat gctcgcttcg gaggacctcg ttgcccagga
     1681 cgccatcagg ttctactccg gtgatgctgc catcggcata atcgggatca ctccgttcac
     1741 ggcgaacctc gaccggcccg agatgcaagc cttcgtcgag gcgtacagcg agcgcaccgg
     1801 caacatcccg accttctggg gagagtcctc atacgtggcg gcgatggtga tcgaccgcac
     1861 actccagtac gtgagtgagg cgaacgacat cgcgctcgca gatctccccg aatgggtgag
     1921 atcgaacggt gaggccttcg ccgcagccac gcgagaaatc gacctgtctg atgcgccgtc
     1981 gtcagccatc actgtggacg agttcaaccg gcaagtgcgg gacttctaca tcgtggagct
     2041 ggtggacgaa ggcggcacca tcaccgacag cctcgtcgag acggtcccgg aagtgactca
     2101 attctggacc ttcgaccaac aggaattcct ggcggacccg ctgttctctc gagaggaccc
     2161 gccggcctag cattgcctgg atacccaaaa tgtccgtcgg ggagccacgt ccacgtcgct
     2221 cccc
//
LOCUS       DATDOZ010000297         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2874725, whole genome shotgun sequence.
ACCESSION   DATDOZ010000297 DATDOZ010000000
VERSION     DATDOZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2874725"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..564
                     /locus_tag="VI541_05955"
     CDS             142..564
                     /locus_tag="VI541_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70483.1"
                     /translation="MFNPRPVRTTGGVERVSVEDEPLGPQAPGNRERGHPAPIGFAPD
                     HPHIPSILRPGIDEGLGVTGDALFGRWRGRAARLAIRKVESHHPMPDPGYQRTEGSKT
                     GAIRISSSSVSQKHRWGRRPVGRLIQMDHMRDLAQTWH"
     gene            complement(549..1394)
                     /gene="mutM"
                     /locus_tag="VI541_05960"
     CDS             complement(549..1394)
                     /gene="mutM"
                     /locus_tag="VI541_05960"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HLF70484.1"
                     /translation="MPELPEVEVIRRDLEKEIIGRKIKRVEVRSTKNAMRIIRRHRKR
                     KDFEDGLAGAKIMKVDRLGKYLLVGLDNGSVLVIHLGMSGQLVLSKSSAPVANHTHVI
                     LEFTVGGQLRYVDPRTFGEMFLSAKDDVGRIKELQDLGIDPLVDALTWQYFSETLAHR
                     KAKMKGILMDQKFISGIGNIYSDEILFLAGIRFDRISDTLSSSEVRRLYRAIQEILQE
                     AVRYRGTSADDEQYRDLYGLPGEFQQFLKVYQREGLACRRCRTPIDRTRWSNRSTYFC
                     PQCQV"
     gene            complement(1395..2090)
                     /gene="rnc"
                     /locus_tag="VI541_05965"
     CDS             complement(1395..2090)
                     /gene="rnc"
                     /locus_tag="VI541_05965"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HLF70485.1"
                     /translation="MALTETGSLFARLGIPQGEDHDLLTLALTHRSYAFEQGGLPHNE
                     RLEFLGDAVLGLAVTDMIYEWYPDLPEGEMAKLRASTVNMAVLADAARRADLGASILL
                     GRGEEMSGGRDKSSILADTFEALIGAVYLSSGMEVARGFIERTLADHIRDHVERGVVR
                     DFKTTLQESVAQSGKVPEYRITATGPDHAKRFIASVWVSGELLGEGGGHTKKEAEQSA
                     AREALTLLREREG"
     gene            complement(2100..2276)
                     /gene="rpmF"
                     /locus_tag="VI541_05970"
     CDS             complement(2100..2276)
                     /gene="rpmF"
                     /locus_tag="VI541_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLF70486.1"
                     /translation="MPLPKRKKSRSTTRHRKSQWKLTSPPNSSCPHCHAPKLPHRVCG
                     KCGYYAGREVVEID"
     gene            complement(2277..2792)
                     /locus_tag="VI541_05975"
     CDS             complement(2277..2792)
                     /locus_tag="VI541_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLF70487.1"
                     /translation="MVIEVGDLLGVPGSHTDVSVTGPIEGLRGALGWVDEDDPVEVVV
                     TVESLADGLAVTGAATGNLHLVCSRCLTEFNMTFEQRIDEAYYLEKAEERGGYEVAGA
                     MLDLEPMLRDVIVLGIPVRPLHSLDCKGLCPECGTDRNTQDCGHSSRAGDIRWAPLAD
                     LAAELKEGREQ"
     gene            complement(2904..>3508)
                     /locus_tag="VI541_05980"
     CDS             complement(2904..>3508)
                     /locus_tag="VI541_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70488.1"
                     /translation="SWPRRSARRAESERRDGVIQVDLIEEIEAMIEEVRNAKNVPLSA
                     SAMINRADMLEKLESLKQNAPEELKQARWVMRDREEVLSRTQEEVNKLKAEAVEERDR
                     LLSRTEIVNAANREADRILDEAKSRAREIRMEAEDYVDAKLANFEVILQKTLQAVGKG
                     RESLRGRLDAASGGGPDALEEEPASEDVTETTGNVRIRRP"
BASE COUNT          638 a         1164 c         1006 g          700 t
ORIGIN      
        1 cttcaggctg tggtcgcccc cctcgaccac ctcgagcctg gcatcgacaa gcagcccgag
       61 gtgatcgtgc agaacctgaa ggtcacagaa ggcgtcgtta gagccctgca gaaataggca
      121 ggggaccctg atatctcccc agtgttcaac ccgaggcctg tccgcacgac cgggggcgtg
      181 gagcgggtat ccgtagaaga tgagcccctg ggcccccagg cccccggcaa ccgcgagcga
      241 ggccacccgg cccccatagg atttgccccc gaccacccac acatcccctc catacttcga
      301 ccgggcatcg acgaaggcct cggcgtaact ggggacgctc ttttcggccg ctggcgggga
      361 cgcgcggccc gcctcgcgat acgcaaggtc gaatcgcacc acccgatgcc cgatcccgga
      421 tatcagcgca ccgagggttc gaagaccggg gccatcagaa tctcctccag ctccgtgagt
      481 cagaagcacc gatgggggcg ccgcccggtc ggtcggctga tccagatgga tcacatgcgg
      541 gacctagctc agacctggca ctgagggcag aagtaggtcg agcggttact ccagcgcgtg
      601 cgatctatcg gagtcctgca gcgccgacat gccaggccct cgcgctgata gaccttgagg
      661 aactgttgga actcaccggg cagaccatag aggtcgcgat actgctcgtc atcggctgag
      721 gtcccccgat agcggaccgc ctcctgaagg atctcttgta tcgctcggta tagacgtctt
      781 acctccgatg aggagagggt gtcggagatc cgatcgaagc ggatccccgc gaggaagagg
      841 atctcgtcgg agtagatgtt cccgatgccg ctgatgaact tctggtccat caggatcccc
      901 ttcatcttgg ccttgcggtg cgcgagcgtc tcggagaagt actgccacgt aagcgcatcg
      961 acgaggggat cgatccccag atcctgcaac tccttgatcc ggccaacgtc atccttggca
     1021 ctcaggaaca tctcgccgaa cgtccggggg tcgacgtacc ttagctgacc cccgacggtg
     1081 aactccaaga tgacatgtgt gtggttggcc acgggcgctg acgacttgga gaggaccaac
     1141 tgtcccgaca tcccgagatg gatcacaaga acagaaccgt tgtcgagccc gaccagcagg
     1201 tacttgccca ggcgatcgac cttcatgatc ttggctccag cgagcccgtc ctcgaagtcc
     1261 ttgcgtttgc gatgacgtcg gatgatgcgc atggcattct tggtagagcg gacttcgacg
     1321 cgttttatct tgcggccgat gatctctttc tcgagatcac gacggatcac ctctacctcg
     1381 ggtagctcgg gcacctaacc ctctctctct cgcaacaggg tcagggcctc gcgcgcggcg
     1441 gactgctccg cttccttctt ggtatgcccc ccaccctcgc ccaacaactc acccgatacc
     1501 cagaccgatg cgatgaacct cttggcgtgg tccggacccg ttgcggttat ccggtactcg
     1561 gggaccttgc cactctgagc aaccgattcc tgaagggttg tcttgaaatc ccgcacgact
     1621 ccgcgctcga cgtggtcgcg gatgtgatct gcgagggtcc gttcgataaa cccgcgagca
     1681 acctccatcc cggatgagag atagacggcc ccgatgaggg cctcgaatgt gtcggccagg
     1741 atcgacgact tgtctcggcc cccagacatc tcttcgcctc tgcccaggag tatcgatgct
     1801 cccagatcgg ctcttcgtgc ggcatccgcc aataccgcca tgttcacggt gcttgcccgc
     1861 agtttggcca tctcgccttc gggcaggtcg ggataccact catagatcat gtcggtaaca
     1921 gccaggccca ggacagcgtc gccgaggaat tcgagccgtt cgttgtgggg caggcccccc
     1981 tgctcgaagg cataggaacg gtgggtcagc gcgagtgtga gcagatcgtg gtcttcgccc
     2041 tgggggatcc ccaaacgtgc gaagagcgag ccggtttcgg tcagtgcgat ggcccccaac
     2101 tagtcgatct cgactacctc acggcccgcg tagtacccgc acttcccaca cacacggtgc
     2161 gggagtttgg gggcgtgaca gtgaggacaa ctcgagttcg gtggagatgt gagcttccac
     2221 tgagacttgc ggtgacgggt cgttgaccgc gacttcttcc tcttcggtaa cggcatctac
     2281 tgttcccttc cctctttcaa ctcagcagcg aggtcggcga gtggtgccca cctgatgtcc
     2341 cccgctctac tgctatggcc acagtcctgc gtgttgcggt cggttccgca ctccgggcac
     2401 aatcccttgc agtccaagga gtgaaggggc cggaccggga tgccaagcac gatgacatct
     2461 ctgagcatcg gctcaagatc caacatcgca cccgccacct cgtacccgcc cctctcctcg
     2521 gccttctcca agtagtaggc ctcgtcgatg cgctgttcga aggtcatgtt gaactcggtc
     2581 aggcacctgg aacagaccag gtgcaggttc ccggtagccg cacctgtaac ggccagccca
     2641 tccgcgaggc tctcaacggt tacaaccacc tcgaccggat cgtcctcgtc aacccacccc
     2701 aatgcgcccc tgagaccctc gatcgggccg gtcaccgaga catctgtatg cgagccgggc
     2761 acacctaaaa ggtcgccgac ctcgataacc aagccgtcca ttatgatccc tgggagaagc
     2821 gaccgccatg ataacaaaac cacaggcctg ccattttctg ccaggtctcg aacgggaagg
     2881 tcaggggcag tctggggccc gtttcagggc cggcggatcc taacgttccc cgtcgtttcg
     2941 gtcacatcct cgctcgcggg ctcctcttct agcgcatcag gccccccgcc gctggccgca
     3001 tccaggcgtc cgcgcagcga ctcacgcccc ttccctacgg cctgaagggt cttctgaagg
     3061 atgacttcga aattggccag tttggcgtcg acgtagtcct cggcctccat ccggatctca
     3121 cgggcgcgcg acttggcctc atccaggatc ctgtcggcct cacggttggc ggcattgacg
     3181 atctcggttc gcgagaggag gcggtcgcgc tcttcgaccg cctcggcctt gagtttgttg
     3241 acctcttcct gcgtgcggct caggacttct tcgcggtcac gcatcaccca gcgagcctgc
     3301 ttgagctctt ccggagcatt ctgcttcagg ctctccagtt tctcgagcat gtcagccctg
     3361 ttgatcatcg ccgacgcgga caggggcacg ttcttggcgt tgcggacttc ctcgatcatc
     3421 gcttcgatct cttcgatcag gtccacctgg atgacaccat cccttcgctc actttcggcc
     3481 cttcttgccg agcgcctcgg ccacgacc
//
LOCUS       DATDOZ010000298         4395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_2601036, whole genome shotgun sequence.
ACCESSION   DATDOZ010000298 DATDOZ010000000
VERSION     DATDOZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601036"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1338)
                     /locus_tag="VI541_05985"
     CDS             complement(91..1338)
                     /locus_tag="VI541_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLF70489.1"
                     /translation="MPKVYNWQLGREMDYRFKGATPELQFAFVFNTNRCLACQTCTMA
                     CKATWTFSRGQEYMWWNNVETKPYGGYPHNWDVKTLEEVERANPGGQSWDGSASPRSP
                     YGVFKGKTIFEAAPTGQEAVGYIPTEAEWRAPNIHEDTAVGRKGKPGAFDPTGTELPE
                     HKTWFFYLQRLCNHCQYPACVAACPRSAIYKRPEDGIVLIDQKRCRGYRKCVEQCPYK
                     KPMYRSDTRVSEKCVACYPRIEGKDPISNGLPMETRCMSVCPGKIRLQGLVKVASDGT
                     WVEDRYNPLYFLVHVEKVALPLYPQFGTQPNGYYIPPRWAPRAYLRQMFGPGVDDAIE
                     RYRDPSRELLAVLQLFRATRTIIFRYEVIPGPKASEATIDGRKHTIFNDTVVGYDRDG
                     NEAVRVSVMEPFYQRPANLLNSI"
     gene            complement(1331..1615)
                     /locus_tag="VI541_05990"
     CDS             complement(1331..1615)
                     /locus_tag="VI541_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70490.1"
                     /translation="MSSHYPEVVDPREVPGPHDPYELVGVELPATGEDVEMMARSFVE
                     EFALLGLTRTRILLLFDTPFFAGSHRLRESLGREKIEAIVSSVLGDDQHA"
     gene            complement(1612..>4395)
                     /locus_tag="VI541_05995"
     CDS             complement(1612..>4395)
                     /locus_tag="VI541_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLF70491.1"
                     /translation="LKFRGGMGLLGVLGKYGMYRVSNQMAVLDTIVRGVPREEALGGR
                     NWSNYTWHGDQAPGHPFVHGLQASDVDFNELRYSRLHIQVGKNLVENKMPESHFFHEV
                     MERGGKIVTIVPEYSAPATKSDYWIPVRPATDTALFLGITKLMIDAGTYDSAFMKAFT
                     DFPLLVRTDNLMRLRAHEAFAGYKPANMSRGPSMKLQGFTGEDRQKIGDFVVMDQRTG
                     RPVAITRDDVGGRLSRRGLDPALEGEFDLTLVDGKTIRVMPLFEAYKLHLKDYDLDTV
                     ADITHAPKHLIERLAKDIAELSPVAIHIGEGINHWFHATEANRSMYLPLMLTGNIGKP
                     GAGCHTWAGNYKSALFQETSWSGPGFNGWIYEDPFEPLLDVKASGKKVKVHKHAKGEE
                     PAYWNHGEMPLVVDTPKQGRKVFTGKTHMPTPTKFLWFTNVNLLNNAKHAYDMLFNVD
                     PKIDTIVSQDIEMTASVEYADIALPANSWMEMQGLEVTASCSNPFLQIWRGGMKPLYD
                     SKDDISIYALLGQKLTEVTGDPRFEKHWRLEIDGRRDVYLRRLLETSATTSAYSLDDI
                     LAGKYGEPGAALMLFRTYPRVPFYEQITDGIPFYTDTGRLNSYCDIPEALEYGENFIV
                     HREGPEATPYLPNVIVSTNSLVRPEDYGIPVGAVGWEERTVRNIKMSWAEASKTKNPL
                     WEQGLHFYCVTPKSRHRVHSQWSVTDWHQIWDSNFGDPYRADKRSPGVGEHQLHVNPQ
                     AAHDLKIADGDYVYVDANPADRPYRGWKKDDFRYKVARLMLRVKYNPAYPYNVVMMKH
                     APFIATERSVRAHESRPDGRAISEGTGYQSNFRYGSQQSITRDWLMPMHQTDHLFHKS
                     KDSMHFVFGYEADNHAVNTVPKETLVRITKAESGGPGGKGKWAPAETGYSPDSESKAM
                     QRYIKGGFLEGER"
BASE COUNT          823 a         1288 c         1299 g          985 t
ORIGIN      
        1 cccctgagag agcaaccgat agatcgctcc tctggcttgc gcgatgacca cctcttcgga
       61 aactgttgct gcttctgaca ccgtcacccc ctagatggag ttgagaagat ttgcaggtcg
      121 ctgatagaag ggctccataa ccgagaccct gactgcctcg ttcccatcgc ggtcgtaccc
      181 cacgacggtg tcgttgaaga tcgtgtgctt gcgcccatcg atggtggctt cgctggcctt
      241 gggtcccggt atcacctcgt agcggaagat gatggtccgt gtggctctga agagctggag
      301 taccgcaagc agctccctcg atggatcccg ataccgttcg atcgcgtcgt cgacaccggg
      361 cccaaacatc tggcgcaggt atgcccgcgg cgcccacctg ggcggtatgt agtagccgtt
      421 gggttgcgta ccgaactgag gatagagggg tagcgctacc ttctctacgt ggaccagaaa
      481 gtagagcggg ttgtatcgat cctcgaccca tgtgccatcc gatgcaacct tcaccaagcc
      541 ctgcaggcgg atcttcccag ggcagaccga catgcaacgg gtttccatcg gcagtccgtt
      601 ggagatggga tccttccctt cgatccttgg gtagcaggcc acacacttct ccgaaactct
      661 cgtatcagag cggtacatcg gcttcttgta ggggcactgc tctacgcatt tgcgataacc
      721 acgacagcgc ttctgatcga tgaggacgat cccgtcttca ggcctcttat agatggcgct
      781 tcggggacag gcggcaacac atgccgggta ttggcagtgg ttgcataggc gctggaggta
      841 gaagaaccaa gtcttgtgct ccggcagctc ggtccccgtg gggtcgaagg ctccgggttt
      901 ccccttcctg ccgacggcgg tgtcctcatg gatgtttggc gccctccact cagcctcggt
      961 tgggatgtac cccaccgctt cttgacccgt tggcgctgct tcgaagatgg tcttgccctt
     1021 gaagactccg tagggcgatc ggggagacgc cgatccatcc catgactgac ctccagggtt
     1081 ggctcgttcc acctcctcta gtgtcttgac atcccagttg tgcgggtaac ccccgtaggg
     1141 tttggtctcg acgttgttcc accacatgta ctcctgcccg cgtgagaatg tccatgtggc
     1201 cttgcatgcc atggtgcagg tctggcaggc aagacatcga ttcgtattga agacgaatgc
     1261 gaattgaagc tcgggcgtcg ctcccttgaa ccggtagtcc atctccctgc ccaattgcca
     1321 gttatagacc ttaggcatgc tgatcatctc ccagaacgct agagacgatc gcttcgatct
     1381 tctctcgacc caacgactcg cggaggcggt gcgaacctgc aaagaaggga gtgtcgaaga
     1441 gcaggaggat ccttgtgcga gtgagcccca gtagagcaaa ctcctcgacg aaagaacgcg
     1501 ccatcatctc gacgtcctcg ccggtggcgg ggagttcgac acccactagc tcgtatgggt
     1561 cgtgggggcc gggaacctct cggggatcga caacctccgg atagtgcgaa ctcatcgttc
     1621 cccctccaag aatccgccct tgatgtatcg ctgcatcgct ttgctctcgg aatccggtga
     1681 ataccccgtc tcggctgggg cccacttgcc cttccccccg ggccccccgg actcggcctt
     1741 tgttattcgc acgagggtct ccttaggaac ggtattcacg gcgtggttat cggcctcgta
     1801 gccgaagacg aagtgcatgg agtctttcga tttgtggaag aggtgatcgg tctgatgcat
     1861 cggcatcagc caatctcgcg tgatcgactg ctgggagccg taccggaagt tggattggta
     1921 accggtgcct tccgagatgg ctcttccatc gggacggctc tcatgtgcac gaaccgaacg
     1981 ctcggttgcg atgaacggag catgcttcat catgacgacg ttgtagggat acgctgggtt
     2041 gtacttcacg cgcagcatga gccgagccac cttgtaccgg aaatcatctt tcttccatcc
     2101 gcggtacggg cggtccgctg ggtttgcatc gacataaacg tagtcaccat cggcgatctt
     2161 cagatcatga gcagcctgag ggttaacatg gagttgatgt tccccgacgc ccggtgatcg
     2221 cttgtctgca cggtaggggt ctccgaagtt cgagtcccag atctggtgcc agtccgtgac
     2281 cgaccactgc gagtggaccc ggtgtcgaga cttcggcgtt acgcagtaga agtgaagccc
     2341 ctgctcccac agggggttct tggtcttact cgcttcggcc caggacatct tgatgttgcg
     2401 aacggttcgc tcctcccaac caactgcccc gacgggtatc ccgtagtcct ccggacgaac
     2461 gagagagttc gtcgacacta tgacgttggg taggtagggg gtcgcttcag gaccttctct
     2521 atggacgatg aagttctcgc cgtactcgag ggcctctggg atgtcgcagt agctattgag
     2581 ccgtcctgta tccgtgtaga aggggatgcc gtccgtgatc tgttcgtaga acggtactcg
     2641 ggggtaggtc cgaaagagca tcagggctgc acccggctcg ccatacttcc ccgcgagtat
     2701 gtcgtcgagt gagtatgcgg atgtcgtcgc agacgtttcg agcagacggc gcagatagac
     2761 atcgcggcgg ccgtcgatct caagacgcca atgcttctcg aatcttggat ccccggtcac
     2821 ttccgtcaac ttctgtccga gcagggcata gatggagatg tcgtccttcg agtcgtagag
     2881 aggcttcatg ccacctcgcc agatctgcag gaaggggttg gaacaggagg ctgtcacctc
     2941 gagcccctgc atctccatcc aggagttggc gggcagggcg atgtcggcat actcgaccga
     3001 ggccgtcatc tcgatgtctt ggctcacgat cgtgtcgatc ttcggatcga cgttgaagag
     3061 catgtcgtag gcgtgctttg cgttgttcag aagattcacg ttggtgaacc acaggaactt
     3121 ggtgggggtc ggcatgtggg tcttgccggt gaagaccttg cgtccctgtt tcggcgtatc
     3181 gacgaccaac ggcatctcgc cgtggttcca gtaggcgggc tcctcgccct tcgcgtgctt
     3241 gtggaccttc accttcttac cagaggcctt cacatcgaga aggggctcga aaggatcctc
     3301 atagatccaa ccgttgaacc cgggacccga ccaggacgtc tcttggaaca gggccgactt
     3361 gtagttcccg gcccacgtat gacatccggc gcccggcttc ccgatattgc ccgtgagcat
     3421 caagggcagg tacatcgacc ggttcgcttc tgtggcgtgg aaccagtggt tgatcccctc
     3481 gccgatgtgg atcgcaacgg gtgaaagttc ggcgatgtcc ttggccagtc gctctatcag
     3541 gtgtttgggg gcatgcgtta tgtcggcaac cgtgtcgagg tcgtagtcct taaggtggag
     3601 cttgtaagcc tcgaagagcg gcatcactct gatggtcttg ccatcgacaa gggtcaggtc
     3661 gaactctcct tcgagagccg ggtctaggcc cctgcgcgat agacgcccac cgacatcgtc
     3721 gcgggttatg gctaccggcc gacccgtcct ctggtccata accacgaagt cgccgatctt
     3781 ctgacgatcc tcgccggtga atccctgcag cttcatgctc ggcccacgag acatgttcgc
     3841 gggcttgtag ccagcaaagg cctcgtgagc tcgaagtcgc atcaggttgt cggtccgaac
     3901 gagcagtggg aagtctgtga aggccttcat gaacgcggag tcatacgtac ccgcgtcgat
     3961 catcagcttc gtgataccga ggaagagggc agtgtcagtg gccgggcgaa ccgggatcca
     4021 gtagtcgctc ttcgttgccg gagccgagta ttcgggaacg atcgtgacga tctttcctcc
     4081 cctctccatg acttcatgga agaagtgcga ctccggcatc ttgttctcca cgaggttctt
     4141 gcctacctgg atgtgcagac gcgagtagcg caactcgttg aagtccacgt cggaagcctg
     4201 cagcccatga acgaaggggt gaccgggggc ctggtcgcca tgccaggtgt agttggacca
     4261 gtttcgcccg ccaagtgcct cttcccgcgg tacccctcga acgatggtgt ccaagacggc
     4321 catctgattc gacactcgat acatcccgta cttgccgaga acccccagca accccatccc
     4381 gccccggaac ttcag
//
LOCUS       DATDOZ010000299         2225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3635118, whole genome shotgun sequence.
ACCESSION   DATDOZ010000299 DATDOZ010000000
VERSION     DATDOZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635118"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VI541_06000"
     CDS             complement(<1..828)
                     /locus_tag="VI541_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HLF70492.1"
                     /translation="MRALNDVPLIRELQRMVTSNPGPINWEIAQQIARAVAGAGKLTH
                     APTKSDIDDFTEDCRIAELQIAARTGSDPSHSITETSVLDRKAWADLNLDILRPLLEK
                     LALHLGQQLTAAPIPQFQSALGALTPFFFGIQVGFLIGYLSRRVLGQSELMLPSSERA
                     PARFVLPNITQAQADLELDAQQFRLWLAMKEVAHRLRYQDVPWGAPYLDSLVTSYIEG
                     GSIDANELRNRIQSLGDAEEISAVLERPEELIPLLATPEQEQTLSRIQVFLSLIEGHV
                     "
     gene            1107..2117
                     /locus_tag="VI541_06005"
     CDS             1107..2117
                     /locus_tag="VI541_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLF70493.1"
                     /translation="MGRDLAIDLGTANTLIYASGRGIVLNEPTVIALNKENGQVLAVG
                     HEAFRMIGRTPGYIVAERPLRRGAISDFDITEKFLRLLMEQAGANRRFGRPRVLICIP
                     SAITEVERRALLDAATAAGARSAHLVEEPVAAAIGAGLPIHEPTGNLVVDIGGGTSEV
                     AVIAMGGIVVNTSVRVGGFDIDDAITRYIRREYAIVVGERTAEQIKIAIGSAFPLVEE
                     EKAEIKGRDLATGLPKVVQITSEEMREAIADPVSQIVQAVVAALASTPPELGQDVLSR
                     GIALTGGGGMLRGLDMLIAEECDVPVHLTERPLECVALGAGKCLEAFSALKDLFIHST
                     RP"
BASE COUNT          420 a          648 c          704 g          453 t
ORIGIN      
        1 gacgtgaccc tcgatgaggc tcaggaaaac ctggatccga ctgagggtct gctcttgctc
       61 tggtgtggcc aggaggggga tgagctcctc aggtcgttcc aacactgcgg atatctcctc
      121 ggcatcgccc agtgattgga tcctgttccg caactcgttg gcgtcgatgc tcccaccttc
      181 tatgtagctg gttacgaggg agtcgaggta gggagcaccc catgggacgt cttgatatcg
      241 aagcctgtgt gccacctcct tcatcgcgag ccacagcctg aactgctgcg catcgagttc
      301 caggtcagcc tgcgcttgag tgatgttggg aagcacgaat cgtgctggtg cgcgctccga
      361 tgacggaagc atcaattcgg attgacccag gacgcggcgc gacaggtagc cgatgaggaa
      421 cccgacctgt atgccgaaga agaatggtgt cagggctccc agggccgact ggaactgcgg
      481 gatcggtgcc gccgtcagct gttgacccag atgcagcgcg agtttttcga gaaggggtct
      541 gaggatatcg agattcaggt ccgcccaggc ctttcgatcg agcaccgatg tctcggtgat
      601 gctgtgggaa ggatccgaac cggttctcgc tgcgatctga agctccgcta tgcggcagtc
      661 ctcagtgaag tcatcgatgt cactcttggt cggagcatga gtcaactttc cggcgccggc
      721 gacagccctg gctatctgct gcgcgatctc ccaattgatc ggccctgggt tggatgtgac
      781 catccgctgc agctcgcgga tgagcggcac atcgttgagc gcgcgcatca ggtcggtcgg
      841 ctccagatca tccatggacc aatactaggc ccagttgaaa ggcggaacga cggaggccga
      901 cgcccagccg aagccggtat agactcatac ccagtggagt acctgctctt cgccgtcatc
      961 cttgcggcga ccctcatcac gatcatctct ctgaagaacc ggccctccaa agatccggct
     1021 cgcagtgtgg aaacgtttcg gcgcgcccgg cgagctctgg acccgaggcg taagccacgt
     1081 cggggggcca agacccctgc gcgttgatgg gtagggatct tgccatcgac ctgggtacgg
     1141 ccaacaccct gatctacgcg agcggccgcg ggatcgtcct caatgagcca accgtcatcg
     1201 ctctgaacaa ggagaacggt caggtactgg ctgtcggaca tgaagcgttc cgcatgatcg
     1261 gtcgcactcc gggatacatc gttgccgaac gtcccttgcg acgtggagcc atcagtgatt
     1321 tcgatatcac cgagaagttc ctgcggctct tgatggagca ggcgggggcc aatcgcaggt
     1381 ttggcaggcc gcgcgttctt atctgcatcc cttcggcgat cacggaagtc gagcgccgtg
     1441 ctcttctaga tgccgcgacg gccgctggcg cgagatctgc tcatcttgtc gaagaacctg
     1501 tcgccgccgc gatcggcgcg ggactgccga tccacgaacc aacggggaac ctcgtcgtgg
     1561 atatcggcgg gggaaccagc gaggtcgcgg tcatcgcgat gggcggcatc gtcgtgaaca
     1621 cttccgtgag ggtcggtggt ttcgacatcg atgacgctat aacgcgctac ataaggcgcg
     1681 agtacgcgat cgttgtcgga gagcgcaccg cggagcagat caagatcgcc atcggctcgg
     1741 ctttccctct ggtagaagag gagaaggccg agatcaaggg ccgcgatctg gcaacgggac
     1801 ttcctaaagt cgtgcagatc acatccgaag agatgagaga ggcgatcgca gatcccgtga
     1861 gtcagatcgt ccaggccgtt gtggccgccc tggcttcaac gcccccggaa ctgggccagg
     1921 atgttctgtc ccgggggatc gctctcacgg gcggaggggg gatgcttcgc ggattggaca
     1981 tgctcatcgc tgaagagtgc gacgtgccgg tgcatctcac ggaacgaccg ctcgaatgcg
     2041 tcgcgcttgg ggcaggaaag tgcctcgagg ccttctccgc gctcaaggac ctgttcatac
     2101 actcgacacg tccgtgaggt tcaggttcac cgtccttctg gacgcgatcc gcgagcatct
     2161 gagttctgcc gccgagatag cgttcttctt gcttttgtcg atcttcccgg gactgcttct
     2221 tgtgt
//
LOCUS       DATDOZ010000300         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1824607, whole genome shotgun sequence.
ACCESSION   DATDOZ010000300 DATDOZ010000000
VERSION     DATDOZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824607"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VI541_06010"
     CDS             complement(<1..871)
                     /locus_tag="VI541_06010"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLF70494.1"
                     /translation="MATTFAPPSALTKLEAQTRAGLIDDVAYLVKLDLTDSDETYESD
                     TTVEFTCTQPGSTTFVDLVSRSVSAIELNGEPIPLDRFVGDRIELANLPAHNTLRVVA
                     TCPFERTGVGLHRFVDPADGRVYVYSDFEPYEARRTFTCFDQPDIKGTFDFEVKAPEG
                     WTVVSCTRASSSPSDGAEGVWIFETTPKMSTYVTAVCAGPWHSVFDEHAGIRLGLFCR
                     QSLAEYLDPEEIFEITKAGFDFYNEEFDFPYVLDTYDHVFCPEYNQGAMENFGCITVA
                     EVYIFRSKVTDTAP"
     gene            complement(894..3374)
                     /gene="leuS"
                     /locus_tag="VI541_06015"
     CDS             complement(894..3374)
                     /gene="leuS"
                     /locus_tag="VI541_06015"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HLF70495.1"
                     /translation="MRQDAVRYLPMVTRYDPKGFEQRWASDWDARSLYAAPRLPGDKP
                     KRFVMEMFPYPSGDLHMGHVENYSIADAMARYWRMKGFEVLHPMGFDSFGLPAENAAM
                     KGNIHPSEWTKANIAKMRSSFLRLGLSYDWSREISSSEPDYYRWNQWIFLKFFERGLA
                     YRKRSTVNWCPVDKTVLAKEQVSTGVCWRCGAVPEARELTQWYLRITDYADRLLEDMG
                     GLDWSESLLSQQRHWIGRSRGAQVTFKVRKTDGTEVDLPIFTTRPDTLWGATFVVLAP
                     EHPLALELVIGSDSEDEFRSFLEATKVKAEVARMADKESRRGMALPAVAINPVNGEEI
                     PIWAADYVLIGYGTGAIMAVPAHDQRDFEFARANDLAVRVVIQPEDETLTADPLTEAY
                     VGPGRMIASGQFDGTVIDPESKAGIDSVIDWLESEAKGEGTINLRLRDWLVSRQRYWG
                     TPIPVIHCEECGTVPVPYEELPVLLPEAVDYGIGEDAVSPLASIPEFVDVKCPSCGRQ
                     AKRETDTLDTFFDSSWYFLRYLDPKDESQPFSHETVAAWGPVDQYVGGTEHAVMHLIY
                     ARFFTKVFQDMGMLDFPEPFLALFNQGNVTLDGREMSKSSGHLVEAAEVLDKLGADSA
                     RTFILFCSPPDADYDFPADGMDAIQRVAFGWLSRVWRVLNDVTPRPADGEIQRALHRT
                     IKAVTEDFETFSFNTAIARLMELVNEFSKLGGPVPRDGAEAFLKLLAPIAPFITEELW
                     HSYGNVDSIHLQRWPGFDQDLADQPRVVMVVQVDGKVRDRIEVAATITQDEMRTLALA
                     SEKIAGLLDGAEPSKVIVVPPKLVNLVR"
     gene            3417..>3959
                     /locus_tag="VI541_06020"
     CDS             3417..>3959
                     /locus_tag="VI541_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HLF70496.1"
                     /translation="MESTESVQDYLKAIYSLGHEEPEVTTSAIAARLGVSSASVTAMV
                     RRLSAAGHVRHKLYQGVELTESGRSLALEVIRHHRLIEAYLHKSLGVPWDRVHQEAEV
                     LEHALSEELEDRISEALGHPTHDPHGDPIPPKKGRYTEIRHRTLGDTAAGPATVERVS
                     DRDASALRYLDRLGLRPGASI"
BASE COUNT          794 a         1200 c         1171 g          794 t
ORIGIN      
        1 cgggagccgt atcggtcacc ttcgatcgga atatgtagac ctctgcgacg gtgatacagc
       61 cgaagttctc catcgctccc tggttgtact ccgggcagaa gacgtggtca taagtgtcga
      121 gaacgtaagg gaagtcgaac tcctcgttgt agaagtcgaa acccgccttg gtgatctcga
      181 agatctcttc agggtcgagg tactcggcga gcgactgtcg gcagaagagt cccaacctga
      241 tacccgcgtg ctcgtcgaag acgctgtgcc acggtcccgc gcataccgcg gtcacgtagg
      301 tcgacatctt cggcgtcgtc tcgaatatcc ataccccctc agcgccatcg ctcggcgatg
      361 aactggcccg ggtgcaactg acgaccgtcc acccctcggg ggctttcacc tcgaagtcga
      421 aggtcccctt tatgtcgggc tggtcgaagc acgtgaatgt gcggcgagcc tcgtatggtt
      481 cgaaatctga gtagacgtag accctcccat cggctgggtc tacgaatcga tggagcccca
      541 cacccgtccg ctcgaatggg cacgtcgcca cgactcgaag ggtgttgtgt gcgggcaggt
      601 ttgcgagttc gatgcggtca cccacgaatc tgtcgagcgg gatgggttcg ccgttgagtt
      661 ctatcgccga gaccgaccgg gaaacgagat cgacgaatgt tgtcgaacct ggctgggtac
      721 aggtgaactc caccgtggtg tcgctctcgt aggtttcatc cgagtccgtg aggtcgagct
      781 tcactagata cgcgacgtca tcgatgagcc ccgcccttgt ctgtgcctct aacttggtga
      841 gcgcgcttgg cggcgcaaaa gtagttgcca tcggatccct ttcgatcggt tacttaccgg
      901 accaggttga ctaacttggg tggaacgacg atcactttag agggctcggc cccatcgagg
      961 aggcccgcga tcttttcact cgccagcgct agcgtgcgca tctcgtcctg cgtgatggtg
     1021 gcggcaacct cgatccggtc acggaccttc ccgtcgacct gtacgaccat gaccactctt
     1081 ggctggtcag ccagatcttg atcgaatcca ggccagcgct gcaggtggat cgagtctacg
     1141 ttcccatagc tatgccatag ctcctctgtg atgaagggag cgatcggtgc cagcaacttg
     1201 aggaatgcct cggcaccgtc gcgcggcacg gggcccccca gtttggagaa ctcgttcacc
     1261 aattccatca aacgagctat cgctgtattg aaactgaatg tctcgaaatc ctcagtcaca
     1321 gccttgatgg ttcggtgcag agccctttgg atctctccat cagcaggccg gggagtcaca
     1381 tcgttcaata cccgccaaac gcgcgacaac cagccgaaag cgacgcgttg gatggcatcc
     1441 ataccatccg ctgggaagtc gtagtccgca tcgggagggc tgcagaagag gatgaaggtg
     1501 cgggcagagt ctgcccccaa cttatcgagc acctcggccg cttcgaccag gtggccggag
     1561 gacttggaca tctccctgcc gtcgagagtg acgttcccct ggttgaacag agccaggaag
     1621 ggttctggga aatcaagcat ccccatgtcc tgaaagacct tagtgaaaaa gcgcgcatag
     1681 atcaggtgca tgaccgcgtg ttcggtaccg ccgacgtact gatcgaccgg accccatgcg
     1741 gcaaccgtct catgagagaa cggctgggac tcatccttgg ggtcgagata tcgcaagaag
     1801 taccacgacg agtcgaagaa ggtgtcgagc gtatcggtct cgcgcttagc ctgacgtcca
     1861 catgaggggc acttcacatc gacgaactct gggatcgatg ccagagggct cacggcatcc
     1921 tcaccgatcc catagtcgac tgcctcaggt agcaggactg gcaactcttc gtacggcaca
     1981 ggcaccgttc cacactcttc gcagtggatc acggggatag gagtgcccca gtatctttgg
     2041 cgcgagacca accaatctct caatcggaga ttgatggtgc cctcaccctt ggcttcggat
     2101 tccagccaat cgatgacgct gtcgatccca gccttgctct cgggatcgat cacagtcccg
     2161 tcgaactgtc ccgatgcgat catcctgccc ggcccgacat acgcctcggt cagcggatcg
     2221 gcggtgaggg tctcgtcctc aggttggatg accacccgca cggcaagatc gttggcgcgc
     2281 gcgaactcga agtcacgttg atcgtgggcc ggcaccgcca tgatggcacc ggtgccataa
     2341 ccgatgagca cgtagtcggc agcccagatg gggatctcct cgccgttgac ggggttgatc
     2401 gccaccgcgg ggagggccat gcccctgcgc gactccttgt cggccatgcg cgcgacttcg
     2461 gccttgacct tggtggcttc gaggaatgat ctgaactcat cctcgctatc gctaccgatc
     2521 acgagttcga gggccagtgg gtgctctgga gccagaacga cgaaggtcgc cccccaaagc
     2581 gtgtcgggac gcgtcgtgaa gatgggtagg tcgacctcgg ttccgtctgt ctttcggacc
     2641 ttgaacgtga cctgtgcacc gcggctgcgc ccgatccagt ggcgctgctg cgaaagcagc
     2701 gactcactcc agtctaggcc ccccatgtct tcgaggaggc gatcggcgta gtcggtgatc
     2761 cgcagatacc actgggtgag ttctctcgcc tcgggcaccg cgccgcatcg ccagcagacc
     2821 ccagtcgaga cctgttcctt cgcgagcacc gtcttgtcga cgggacacca gttcacggtt
     2881 gagcgcttgc ggtaggcgag acctcgttcg aagaacttga ggaagatcca ctgattccag
     2941 cgatagtagt cgggctcact gctactgatc tcgcggctcc agtcgtaaga gagccccagg
     3001 cgcaggaatg acgacctcat cttggcgatg ttcgccttcg tccactcgga tggatggatg
     3061 ttgcccttca tggccgcgtt ctcggcgggc agcccgaacg agtcgaaccc catcgggtga
     3121 agaacctcga agcccttcat ccgccagtat cgagccatag catcggcgat cgaatagttc
     3181 tcgacatgtc ccatgtgaag atcgcccgag gggtagggaa acatctccat cacgaagcgc
     3241 ttgggtttat cgccgggcaa cctgggcgcg gcgtagaggc tccgagcatc ccaatcagat
     3301 gcccatctct gctcgaatcc cttcggatcg tacctggtga ccatgggcag ataacgtacc
     3361 gcatcttgtc tcatctcttg acacaaatga tttagatgtg actaaactag ggattcgtgg
     3421 aatctacaga gtcggtccag gactacctca aggcgatcta ctcgctcggc cacgaggagc
     3481 ctgaggtgac cacatcggcg atcgcagcac gcctcggtgt ctcctcggcc tcggtcactg
     3541 cgatggtccg ccgactcagc gccgcgggac atgtccggca caagctctat caaggggtag
     3601 aactgaccga gagcggccgc tccctcgcgc tcgaggtcat cagacatcac cgcctcatcg
     3661 aggcatacct gcataagagc ctcggggtac cgtgggatcg agtgcatcag gaggccgagg
     3721 tcctcgaaca tgctctgtcg gaggagttag aggaccgcat ctcggaagct ttggggcacc
     3781 cgacgcacga cccacatggg gaccccatcc caccgaaaaa gggccgctac accgagattc
     3841 ggcatcgcac gcttggcgat acggcggcag gcccggcgac cgtggagcga gtctcagaca
     3901 gagacgcaag tgcgcttcgg tatctcgata ggcttggcct acggccggga gcctctatc
//
LOCUS       DATDOZ010000301         5904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_253978, whole genome shotgun sequence.
ACCESSION   DATDOZ010000301 DATDOZ010000000
VERSION     DATDOZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253978"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VI541_06025"
     CDS             <1..503
                     /locus_tag="VI541_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLF70497.1"
                     /translation="LTRGEADAAADAARSSLAAIEPRFTRAPYLDVIWVAGRVLIGQQ
                     APEAQDLRNEILEDLFMIDKSTVDPEIKSRFFALPRISELAELCGFAGFQIELPAPWL
                     EAGLDESEMMILQQLVTGKSDAEIASTIDVTEANVGEQLDVIFAKLGVSNRTAAKEYA
                     LTGGIA"
     gene            500..706
                     /locus_tag="VI541_06030"
     CDS             500..706
                     /locus_tag="VI541_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HLF70498.1"
                     /translation="MTIKVIIDRRRCIGAGNCIYLAPTIFRWREGDLLKPELLDPTTV
                     EEDVLREVAASCPTNAILVEEVDE"
     gene            complement(737..2557)
                     /locus_tag="VI541_06035"
     CDS             complement(737..2557)
                     /locus_tag="VI541_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLF70499.1"
                     /translation="MKKIRAGLICVTLLTAIVLTTTASANHTATHCVADAYHAQSLAG
                     PVATNTALPANSELLMRFVNVSDTHIIDDEASTAMNGTWLESLLEPAIGNGAAQRLQE
                     EYSDEVLNALVKTINGCKQQLQDLELMIATGDLTDNMTLNELRRYIDNLDGVTGADTA
                     FEANCGYKTKDSLGRSKGGLPCTPELQQAAALMTGKLVPDSQTTPPDVTGTAGTAYQL
                     TPTRSALQIANTTLASEIGLSHNIAPGLPPRLRCNPEVQLNCDNLKLAIPHYAVFGNH
                     DGSVRGTLTMQRPFQAGAAHFGRYFIENQREFINEWFKTESLPGPVGHGFEYVDASRW
                     TDQNDRNDGYYAFDAGANSSVRMIVLNTIFDGVRQELHRDGQTATDTQGLVGGNEVTP
                     APATSEQGFVDQTQFNWLQGQLAAAQTANQAILVFGHHPDRSFSYAEYGSKLPQQFDT
                     LLGTYPNVVAYIAGHTHENVIRPCTPTDCLVKGIQPTIANSFWRIETSSMIDFPQESR
                     IIEVYRLPGGEFAIRSTMIQADQSDADASLSHRLAVAEAECKTSHLLGGPLSSGPYDQ
                     QRLQATLARAGEAAQRSNFCIQGIDMSAGQPKDRDTLLLP"
     gene            complement(2638..3681)
                     /locus_tag="VI541_06040"
     CDS             complement(2638..3681)
                     /locus_tag="VI541_06040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF70500.1"
                     /translation="MARIHLVYPVPPPAAGQTGPLAGLRYRAGAALDRRRPSRYREGR
                     EFSRALPAPTSITANLFKHLSERHRTLLYDWSEHGAIDIADSDILIGHPHPTHGKVFS
                     TTMARMTRGRVVMMPLHHAMPQISAFAIEQLQSADLILGLMGEHWYDTIDGTFLAPFK
                     EKIVRLDMAIDATSYPFLRERFNPPGRRGILWVGANRPEKGVDLLSRIAARSPGVPRG
                     WIGPGASIPHIHHIAAHARLTPSYVRHLMERYDIFVNTSISDANPTTVLEAMAWGFPL
                     ACTPGSGYQGVGSIETLDPNDIEACVATVARLQQADEGALQEAVAVNRRLVQEFYTWE
                     RFCLTVDGGLAQL"
     gene            3725..4918
                     /locus_tag="VI541_06045"
     CDS             3725..4918
                     /locus_tag="VI541_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLF70501.1"
                     /translation="MDRLRVAFDASLVPGGSAGGVEHYVRSLILAMSRIGEVDMTYVT
                     SWAAPDWTDAMRSPTQRIAVKPSPYPGAGLIPVRLRPALADLRNRRVSRRAGSLIDTD
                     VDPFFDGLGDVVHFPAHRFARTTKPSLYCVHDLLHRHWPQFLPPREVERREANLSFAI
                     EEATIITTHSKWAASDVVANYEVAAQEVRPIPIGASTDNYPRVEPGRADAVIKGLGIE
                     GEFALYPAQTWPHKNHIRLIDAMKIAIAAGSDITLICTGAKNEHWPQIEDHIHGSGVE
                     GRVVFTGYVKEETMVSLYSRARMVVFPSLFEGGGLPILEALRFGVPLTCSNVTSIPEY
                     AGEAAHYFDPLSTEEIANALIEVNDNEELRDRLSARASNQIGQFDWGRSARVCVDLYR
                     EVAGA"
     gene            complement(4915..>5904)
                     /locus_tag="VI541_06050"
     CDS             complement(4915..>5904)
                     /locus_tag="VI541_06050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF70502.1"
                     /translation="GEPLVSIVTPCRDAVDMVGACIESIRTQSYPNIEHIVIDAASTD
                     GTVELLEGHEQITWVSEPDSGQSNALNKGFAMAKGEYLAWLGADDLLKPRSVEWTVRR
                     LFAAGSGIAYGDVEFVEPGRTYIVKPPARLSSRSFEMFNPIWQPGTVFTAEAFKRVGG
                     IDETYNLAMDFDLWLRMVDAGITATYVPETLATYVVHDASKTGALNREDFIIEMFRSY
                     LRSGRVREASLALGRAAANAAHQDGVVDVAATQENLDRYISMLDPGRELSLKIARAGA
                     WAEASVLELQRSVRGLRHVLRLSPWLVGPTRRRLLLAARRGLTRGRLAVPDNT"
BASE COUNT         1082 a         1740 c         1875 g         1207 t
ORIGIN      
        1 gcctgactcg cggggaggca gatgccgcgg ccgatgccgc gcggtcttct cttgccgcta
       61 tcgagccgcg attcactcgt gcgccctacc tcgacgtgat ctgggtggcg gggcgagtgc
      121 tcatcggcca gcaggccccc gaggcccagg acctgcgcaa cgagatcctc gaagacctct
      181 tcatgatcga caaatcgacc gttgatcctg agatcaagag tcggttcttc gccctgccaa
      241 ggatcagtga gctcgcagag ctctgcggct tcgcgggatt ccagatagaa cttcctgcac
      301 cctggctcga ggcgggactg gacgagagcg agatgatgat ccttcaacag ttggtaaccg
      361 gtaagagcga cgctgagatc gcttcgacga tcgatgtaac tgaggccaac gtcggcgagc
      421 agctcgatgt catctttgca aagttaggcg tgtcgaatcg caccgctgcg aaggaatacg
      481 ccctgactgg agggatcgca tgacgatcaa ggtgatcatc gatcgccgcc gatgcatcgg
      541 tgcaggcaac tgcatctacc tcgcgccgac gatattccgc tggcgagaag gcgatcttct
      601 gaagcctgaa ctactcgacc cgaccacggt tgaggaggac gtcttgcgtg aggtagctgc
      661 ctcgtgcccg acgaacgcga tcttggtgga agaggtcgac gagtaggggg ccctcttcca
      721 gacgtccgtc gagaagtcag ggcagcagca gagtgtcgcg gtccttaggc tgtccagccg
      781 acatgtcgat cccttgtatg cagaagttgc ttctttgggc ggcctcgcca gctcttgcaa
      841 gcgtagcctg aagccgctgc tggtcgtatg gcccggaaga cagcggccct cccagcagat
      901 gtgacgtctt gcactcggcc tctgccaccg ctagtcggtg cgagagagat gcgtcggcgt
      961 cgctctggtc ggcctggatc atcgtcgagc ggatcgcgaa ctcgcccccg ggcagccggt
     1021 acacctcgat gatgcgactc tcctgcggga agtcgatcat cgacgaggtc tcgatccgcc
     1081 agaaactgtt ggctatcgtg ggttggatgc ccttgaccag gcaatcggtc ggggtacagg
     1141 gacggatgac attctcatgg gtgtgaccgg cgatgtacgc gacaacgttg gggtacgtgc
     1201 ccagaagggt gtcgaactgc tgcggcagct tgcttccata ctcggcgtag ctgaaggacc
     1261 gatccgggtg gtggccgaaa acgaggatcg cctggttcgc tgtctgcgcg gccgccaact
     1321 gcccctggag ccagttgaac tgtgtctgat cgacgaatcc ctgttctgat gtggcaggtg
     1381 cgggggtaac ctcgttgccg ccgacgagcc cctgcgtgtc cgtagcggtt tgaccgtcgc
     1441 gatgcagttc ttggcgcact ccgtcgaaga tcgtgttcag gacgatcatg cgcacggagc
     1501 tgttcgctcc ggcgtcgaaa gcgtagtagc catcgttgcg gtcgttctga tcggtccagc
     1561 gcgaggcgtc gacgtactcg aaaccatgtc cgaccgggcc tggaagcgat tcggtcttga
     1621 accactcgtt gatgaactca cgttggttct cgatgaagta acggccgaaa tgggccgctc
     1681 cagcctggaa gggccgttgc atggtgagtg ttccgcgaac cgaaccgtca tggttgccga
     1741 aaacggcgta gtgggggata gcgagtttga ggttgtcgca gttcaactga acctctgggt
     1801 tgcagcgcag tctcgggggt agtcctggcg cgatgttgtg agacaggccg atctcactcg
     1861 cgagggttgt gttagcgatc tggagcgccg agcgcgtcgg cgttagctga taggcggtgc
     1921 cagcggtgcc agtaacgtcg ggaggagttg tctgactgtc gggcaccagc ttcccggtca
     1981 tgagcgcggc ggcctgttgc agctcgggtg tgcaggggag gccccccttt gaccttccga
     2041 gcgaatcctt cgtcttgtat ccgcagttgg cttcgaacgc tgtgtccgcc ccggttacgc
     2101 catcgaggtt gtcgatgtag cgcctcaact cattgagagt catgttgtcc gtgaggtcac
     2161 cggtcgcgat catgagttcg aggtcttgaa gttgttgttt gcagccgttg atggtcttga
     2221 ccagggcgtt gagtacctcg tcgctgtact cctcctgaag acgttgggcg gctccgttgc
     2281 cgatggcggg ctccaagagc gattcgagcc aagtgccgtt catagcggtc gaggcctcgt
     2341 catcgatgat gtgggtgtca cttacgttca cgaaccgcat gagaagttcg gagttcgcag
     2401 gcagtgccgt gttggtcgca accggcccgg cgaggctctg cgcgtgatac gcgtcggcca
     2461 cgcaatgtgt agccgtgtga ttggccgagg cagtcgtcgt tagaactatc gccgtcagca
     2521 gggttacgca gatgagtcca gcacgtatct tcttcaatcc cagttcctcc ccaggtcgcg
     2581 gcccttccaa actgctagat aagttcgcca tcgagtggcc atatcctgcc ccattgccta
     2641 aagctgtgcg aggcccccat ccacggttag gcagaaccgc tcccaggtat agaactcctg
     2701 aaccaggcgt cgattcacgg ccaccgcttc ctgcagggcc ccctcgtccg cctgctggag
     2761 tctcgctacc gtcgcaacgc aggcttcgat gtcattcgga tcgagtgtct cgatcgagcc
     2821 gacgccctga tacccggacc ccggggtgca agccaagggg aatccccaag ccatcgcttc
     2881 gagcacggta gtggggttcg catccgagat agaggtgttg acgaagatgt cgtagcgctc
     2941 catgaggtgt cgcacgtaag aaggggtgag ccgggcatgg gcggcgatgt ggtggatgtg
     3001 cgggatcgag gcccccgggc cgatccaacc ccgcggtaca ccgggggacc gagccgcgat
     3061 ccggctgagc aggtcgaccc ctttctcggg ccggttcgcc ccgacccaga ggatcccccg
     3121 acgaccggga gggttgaatc gctcccttag gaacgggtac gacgttgcgt cgatcgccat
     3181 gtcgagtcgc acgatcttct ccttgaaggg ggcgaggaat gttccatcga tcgtgtcgta
     3241 ccagtgctcg cccatcagcc caaggatgag atcggcgctt tgcagctgct cgatcgcgaa
     3301 cgcactgatc tgcggcatcg cgtggtgaag aggcatcatg acaacccggc cccgcgtcat
     3361 gcgagccatg gttgtcgaga agaccttgcc atgggtcggg tgtgggtggc cgatcaggat
     3421 gtcactgtcg gcgatgtcga tggcaccgtg ctcggaccag tcgtagagaa gggttcgatg
     3481 gcgttcgctc aagtgcttga agagatttgc tgtgatcgat gtgggtgccg gaagggctcg
     3541 tgagaactct cggccttccc gatatctcga gggtcttcgt cggtcgagcg ccgctccggc
     3601 tcggtatctg agaccagcga ggggccctgt ctgacctgcc gccggtgggg ggacgggata
     3661 gacaaggtgg atgcgcgcca tcagatggtg agtgggacgg tcatgtcttc tataccctac
     3721 cgatgtggat aggttgagag tcgcgttcga cgcgtcgctg gtcccgggcg gctctgcggg
     3781 cggcgtggaa cattacgtcc gaagcctcat cctggcgatg tcgaggatcg gtgaggtcga
     3841 catgacctat gtgacctcat gggcggcccc ggattggaca gacgccatgc gatcaccaac
     3901 tcagaggatc gcggtgaagc cctcgccata tccgggggcc ggtctgatac ccgtccgatt
     3961 acgtcctgcg ctcgcagacc tgcgcaaccg gcgagtctca cggcgtgcag gcagtttgat
     4021 cgataccgac gtcgacccgt tcttcgacgg gctgggagat gttgtccact tccctgccca
     4081 tcgattcgcc agaaccacaa agccgtctct ttactgcgtc cacgacctgc ttcaccggca
     4141 ctggccgcag ttcctgcccc ctcgtgaggt tgagcgccgg gaggcgaacc tctccttcgc
     4201 gatcgaggag gcgacgatca tcacgacaca ctcgaagtgg gccgcatctg atgttgtagc
     4261 caactacgag gttgccgcgc aggaggtgcg tcccatcccc atcggggctt cgaccgacaa
     4321 ctacccccgt gtcgaacctg gtcgcgcgga tgctgtcatc aaagggttag gcatcgaagg
     4381 cgagttcgcg ctatatcccg cgcaaacctg gccgcacaag aaccacatcc gattgatcga
     4441 cgccatgaag atcgcgatcg cggcgggctc ggacatcacc ctgatctgca caggtgcgaa
     4501 gaacgaacac tggcctcaga tcgaggacca cattcacgga tccggcgtgg agggccgtgt
     4561 cgtcttcact ggatacgtga aggaagaaac gatggtctcg ctctactcgc gagcgcggat
     4621 ggtggtcttc ccctctctct tcgagggtgg gggcctgccg atactcgagg ccctgcgttt
     4681 cggggtacca cttacatgtt cgaacgtcac ctcgatcccc gaatacgctg gcgaggccgc
     4741 ccactacttc gacccgctat cgaccgagga gatcgcgaac gcactcatcg aggtgaacga
     4801 caacgaggag ttgagagacc gtttgagcgc gcgagcgagc aaccagatcg gccagttcga
     4861 ctgggggagg tccgcccgcg tctgcgtcga tctctaccgc gaggtggcgg gggcctaggt
     4921 gttgtcgggc acagccagcc gacctctggt caggccacgt ctggcggcaa ggaggagcct
     4981 gcggcgagtc gggccgacca gccagggcga gagtctgagg acatgccgca ggcctctcac
     5041 ggagcgttgt agctccagaa ctgatgcctc tgcccaggcc cccgcacggg cgatcttcag
     5101 agagagttcc cgccccggat cgagcatcga gatgtatctg tcgagatttt cttgcgtcgc
     5161 ggcgacatcc acgaccccat cttgatgtgc ggcgttcgct gcggcccttc cgagcgccag
     5221 ggatgcctcg cgcaccctcc cagaccgaag ataagaacga aacatctcga tgatgaagtc
     5281 ttcgcggttc aaggcccctg tcttcgaagc gtcatggacc acgtatgttg cgagtgtctc
     5341 aggtacgtag gtggctgtga tccccgcatc gaccatcctc agccaaaggt cgaagtccat
     5401 cgccaagttg taggtctcat cgatgccccc tactcgcttg aacgcctcag cggtgaagac
     5461 cgtcccaggc tgccatatcg ggttgaacat ctcgaaggat cggctcgaaa gccgcgctgg
     5521 cggcttcact atgtaggttc gaccaggttc gacgaactcg acatcaccgt aggcgatacc
     5581 gcttcccgct gcgaatagtc tgcggacagt ccactcgaca gaacggggct tgaggaggtc
     5641 gtcggctccg agccacgcta ggtactctcc cttggccatc gcgaacccct tgttgagggc
     5701 attggattgg ccggagtctg gctctgatac ccacgtgatc tgctcatgcc cttcgagtag
     5761 ttcaacggtc ccatccgttg atgccgcgtc gatgacgatg tgctcgatgt ttggatatga
     5821 ctgcgttcgg atactctcta tgcacgcgcc caccatgtcg acagcatcgc gacatggggt
     5881 cacgatcgag acgagaggct ctcc
//
LOCUS       DATDOZ010000302         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_1041101, whole genome shotgun sequence.
ACCESSION   DATDOZ010000302 DATDOZ010000000
VERSION     DATDOZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041101"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VI541_06055"
     CDS             <1..249
                     /locus_tag="VI541_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF70503.1"
                     /translation="VLDRDETTCGRQPTSDIFLDDVTVSRKHAEIRREGTSFTIADVG
                     SLNGTFVNRARVDVSPLANGDEIQIGKFKLAFFTEAAP"
     gene            249..968
                     /locus_tag="VI541_06060"
     CDS             249..968
                     /locus_tag="VI541_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLF70504.1"
                     /translation="MSATRTSDSLPIGEVLKLLADEFPEISISKIRFLESEGLIMPER
                     TPSGYRKFSPNDVGRLRFILKAQRDQFMPLKVIRRKLQHFDPTAPEPETATELNTDPD
                     EDDLPTVNLDGGMSLTFDELIRASGLPADELTQLEEFGLLDAHSLGDGSVIYDEADLA
                     VARIAKDFAKYGIGPRHLRMYKVFADKEAGTYAPIAATFSRSGTEGRKQAKKSLAHVG
                     DLSKRLKQLLFEAALREYLSQ"
     gene            1013..>2660
                     /locus_tag="VI541_06065"
     CDS             1013..>2660
                     /locus_tag="VI541_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLF70505.1"
                     /translation="MPWLTELIGTTVADSQGRKIGRVRDVVAERTRFPTVTGLIVQVD
                     DPDLVPEPGVRIGYVAWQDLSRDYILRERSRFERVQRGDRLFISEELLDQQIVDIDGA
                     RVVRVNDVLLQESAGGLRVVGVDVGLRGFLRRLGLENLATRMADALAYQIPGGLIAWN
                     YVAPLGESGGEVRLNVPTRMLRELHPSELADILDQLDGERRERILRVMSVGELAETLA
                     ETEPHISREAVELLGEERARRVIESMPPDEATDLLGIIGYQRSEHLLSLMGVHKASQL
                     RELLGYPPDTAGGRMTPAFISVLGELTVAAVIDRIRSEAALAETVNYAYVVDRDARLE
                     GVFSLRDLLRAPGDLAISELMQPDVVVVELDDDQEEVARAMTRYNLLAIPVVDSGFVL
                     KGIVTVDDVVDVLEEEASEDLAQVSGVYVGTGGATSSRVVGFGSALLGGLAGTLLLRP
                     HGTVLASLVAVAWLLPLYLRTTLDLGTWSLARSLGAFDLDPKTRLDTLAHELAASLGS
                     AAFSGALVAGFSALWTRSASVAIFLGIGIFVGSLAASLIGL"
BASE COUNT          513 a          823 c          796 g          528 t
ORIGIN      
        1 gtgctcgacc gcgatgagac cacctgcggc cgtcaaccca cgagcgacat cttcctcgac
       61 gatgtcacgg tttcgcgcaa gcacgccgag atcaggcgcg agggcacgag cttcaccata
      121 gccgacgtgg gtagcctcaa cggaaccttc gtgaaccgtg ctcgtgtcga cgtgtctccg
      181 ttagccaatg gcgacgagat tcagatcggc aagttcaagc tagcgttctt cacagaggcg
      241 gcgccctgat gagcgccact cgcacctcgg attcactgcc gataggcgag gtcctcaaat
      301 tgctcgccga tgaattcccc gagatctcta tctccaagat caggttcctc gaatcagagg
      361 gcctgatcat gccagagagg actccttcgg gctaccggaa gttcagcccg aacgacgtcg
      421 ggcgccttcg cttcatcctg aaggcacagc gcgaccagtt catgccgctc aaggtcatcc
      481 gccgcaaact ccaacatttc gacccgacag cccccgagcc ggagacggcc acagaactca
      541 acacagaccc cgacgaggac gaccttccga ccgtgaacct cgatgggggc atgagcctca
      601 cgttcgacga gctcatcagg gcgagtggcc tacccgcgga cgagctgacg caactcgaag
      661 agttcggact tctcgatgcc cactccctgg gtgacgggtc cgtgatctac gacgaagcgg
      721 acctcgctgt cgcgcgtatc gccaaggatt tcgcaaagta cggtatcggt ccacggcatt
      781 tgaggatgta caaggtcttc gccgacaagg aggcggggac atacgcaccg atcgccgcta
      841 cgttcagccg cagcggaacc gaaggtcgca aacaggccaa gaagtctctg gcccatgtgg
      901 gggacctgtc gaaacgtctg aaacagctct tgttcgaagc agcgctgcgc gaatatctgt
      961 cccagtaaca cacccgacca gcccgctagt agttaccgtc cggagacgcg ccatgccctg
     1021 gttgaccgag ttgatcggaa ccaccgtcgc cgactcacag ggtcgcaaga tcgggcgcgt
     1081 tcgcgatgtt gtcgccgaaa gaacgaggtt cccgacggta actggtctga tcgttcaagt
     1141 cgatgatcca gacctcgtcc ccgagcctgg cgttaggatc ggttacgtcg cctggcagga
     1201 tctctctcgc gactacatct tgagggagcg atcccgcttt gagcgcgttc aacgcggtga
     1261 ccgactattt atcagcgaag aactactcga ccagcagatc gtagatatcg acggtgctcg
     1321 tgtggttcgc gtgaacgacg tcctgcttca ggagtcggcc ggagggctgc gggttgttgg
     1381 cgttgacgtc ggcttgcgag ggttcctccg tcgacttggc ctagagaatc tcgcaacgcg
     1441 catggccgat gctcttgctt atcagatccc cgggggcctt atcgcctgga actacgttgc
     1501 ccctctagga gaatcgggag gagaggtacg cctcaacgtg ccaacacgga tgctgcgcga
     1561 gcttcacccg agcgagttgg ccgacatact cgatcagctc gatggcgaac gccgcgagcg
     1621 gatccttcgc gtcatgagcg tgggtgagct ggccgagact cttgccgaaa cggaacccca
     1681 catctcacgc gaagccgtgg agctcctcgg cgaagagaga gctaggcgcg tcatcgagtc
     1741 gatgccccca gacgaggcga ccgaccttct cgggatcatc gggtatcagc gttccgaaca
     1801 ccttctgagt ctcatggggg tccacaaagc ttcacagctg agggaacttc tcggatatcc
     1861 gccggacacc gctggcggac gcatgacccc ggcgttcata tcggtgctag gagagctcac
     1921 ggtggctgcg gtcatcgatc gcatcagatc cgaagccgca cttgccgaga cggtcaacta
     1981 tgcatacgtg gttgatcgcg atgctcgcct cgaaggagtc ttctctctgc gcgaccttct
     2041 ccgcgctccc ggggaccttg ccatatctga attgatgcag cccgacgtgg tcgtcgtcga
     2101 gctcgatgac gaccaggagg aagtcgctag agcgatgacc cgatacaacc tcctcgccat
     2161 cccggttgtc gactccggat tcgtactcaa ggggatcgtc actgtcgatg acgttgtaga
     2221 cgttctcgaa gaggaggcat cggaagacct cgcccaggtc tctggtgtct acgtcggcac
     2281 cgggggagct acctcgagcc gagttgtggg gttcggctcg gcgctactgg gaggcctggc
     2341 agggacactg ctccttcggc cacacgggac ggtgctggct tcgctggtcg ccgtcgcatg
     2401 gctgcttccg ctataccttc gcaccacact ggacctcggt acctggtcgc tcgccaggtc
     2461 actcggtgca ttcgacctcg acccgaagac ccgactcgat actcttgcgc atgaactggc
     2521 ggcttcgctg ggatctgccg cgttctcggg ggccctggtt gcggggttct ccgctctctg
     2581 gacgcgcagc gcatcggtgg cgatcttcct tggtatcggg atcttcgtcg gttcgttagc
     2641 agcttcgttg atcggacttg
//
LOCUS       DATDOZ010000303         6663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3639525, whole genome shotgun sequence.
ACCESSION   DATDOZ010000303 DATDOZ010000000
VERSION     DATDOZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3639525"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VI541_06070"
     CDS             complement(<1..440)
                     /locus_tag="VI541_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HLF70506.1"
                     /translation="MSVQRRHLLFLTVISAAVILVTSIPAIAQECPPNDASCAPSIAS
                     LESQLLDLVNGERTGAGLSALSVNSWAASIAHAWSEKMRADADISHDDSYFAQARSNM
                     PGARLLGENVAMGGSVQRIHDLFMQSPSHRENVMEPRFTHVGIG"
     gene            complement(632..714)
                     /locus_tag="VI541_06075"
     tRNA            complement(632..714)
                     /locus_tag="VI541_06075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Ser,seq:tga)
     gene            complement(785..2035)
                     /gene="serS"
                     /locus_tag="VI541_06080"
     CDS             complement(785..2035)
                     /gene="serS"
                     /locus_tag="VI541_06080"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLF70507.1"
                     /translation="MIDLRLLRSDLERVRASLELRGSKIDIDALVELEARCRELTASV
                     EKDRAAHNEANRQIAQTAPDQRESAIAAARELAERFKGAEAQLQKAREELDAAAATMP
                     NLVYPQAPPGSGDESNLLVRTVGEVKTFDFEGRDHLTIGESLDIIDVERAAKVSGSRF
                     GMLKGKGALLEFALVRLVMDRLMAHGFIPVIPPVLVREEAMYGTGFFPTDEQQVYKTE
                     ADDLYLAGTAEVPLASMHGGETFDADKLPLRYAGFSTCFRREAGTYGKDTRGIIRLHQ
                     FDKVEMFSFCRAEHSDGEHLMLVGLEESIFQALEIPYRVIEICAGDLADPNYRKFDIE
                     AWLPGTQRWLEVTSCSNDIDYQARRLGIRAKGATGAEFVHTLNGTAVAVQRTIVAILE
                     NHQQADGSVLLPAALRPYTGFDSL"
     gene            complement(2007..2525)
                     /locus_tag="VI541_06085"
     CDS             complement(2007..2525)
                     /locus_tag="VI541_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HLF70508.1"
                     /translation="MDTRLLVYVAMAFAMLSLVVAISAHRRFSAARRALLLLQDTYDG
                     KTILDAVSTYLERVDTIRGDLAHEARRIEEVFAILGRSARNLGVVRYDAFEDMGGKMS
                     FSAALLDDHASGVVITSINARSESRVYAKVLLNGDSEHNLSDEERRAIQEALGRKQKV
                     GRLLDRSATSSK"
     gene            2588..3340
                     /gene="larB"
                     /locus_tag="VI541_06090"
     CDS             2588..3340
                     /gene="larB"
                     /locus_tag="VI541_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HLF70509.1"
                     /translation="MDEASIRLLFEKVRSGETSIDDATASLAGLPFSDLGYAMLDHHR
                     ELRTGLAESVYGPGKTPDQVAGIAQDLHAQRGGAVLVTHASVEQFRATRERVPECTFD
                     ELTGMLVVRRSGAEPAGVVVVASAGTSDQKVARECLITLDALAIKGEAIEDVGVAGIH
                     RLFASSEALDRADVVIAIAGMEGALASIIGGLVSVPVIAVPTSVGYGAGAGGIAALLT
                     MINSCAPGVAVVNIDNGYGAAVFAATILRARS"
     gene            3337..4491
                     /gene="larC"
                     /locus_tag="VI541_06095"
     CDS             3337..4491
                     /gene="larC"
                     /locus_tag="VI541_06095"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HLF70510.1"
                     /translation="MIAYLDCFSGAAGDMILAALIDAGADASSVRKSVDLLGLGASIE
                     FEDVSRAGIRGVHTRVTGDDSLAPRTFAEARALIDHAELGDSVRARSLSILTALARAE
                     AKVHGVTIEQVHFHEIGALDTLIDIIGVSSAMSELGLEGFMVGPIATSIGSIESQHGI
                     LPLPAPAVTALLEGFELVGRAGTQELVTPTGAAIIAATGTPARTFPSMKIVTTGYGAG
                     ARDLETPNLLRVIIGEARSVHADEDAVQIEANIDDMNPEYFGYVFERLFAAGALDVWT
                     SPIVGKKGRPANIIGILGPVSAEMALRDVVLTETSSIGIRVTPVRRWILDREWVQVTV
                     EGMAVRVKVARDGGRVVNISPEYSDCEAVARETGIPLKEVFRLALGSLRL"
     gene            complement(4503..5618)
                     /locus_tag="VI541_06100"
     CDS             complement(4503..5618)
                     /locus_tag="VI541_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70511.1"
                     /translation="MSRHARSILGVFVACATLVAMFVPGLASATPRTVQAGPNNSNSF
                     SPNVLSASAGDTVTWNWNPTFQTPPEHTVTGWTGSGSYTDFNSPTLSQTNPSYTATYN
                     GGIIFYRCTNHSWADSDFPGYEGVCHGMCGVVTDVSPSAPPATPTITGPAEGSSTTRP
                     DKVVFTGTGTPWSMVIIFNAGTSAPVTRVLVRGGGTFSVQVAMPAGNYAVFGKAYWAF
                     DAEQPNTSLQSGSSNTRTFSVLANNDISPPGALVTTADGTIFLNEPTIVGEAWDNTGI
                     SQIRVYFRNASGLHLEPFLIATCDDCPGTGNYTHVHWRVTHKSLYSYLTQSLNNGFLN
                     YGVYKVNAIAIDTGGTQGTNFSNTITFIAAPRGPIVQ"
     gene            complement(5780..5862)
                     /locus_tag="VI541_06105"
     tRNA            complement(5780..5862)
                     /locus_tag="VI541_06105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5827..5829),aa:Leu,seq:cag)
     gene            5957..>6663
                     /locus_tag="VI541_06110"
     CDS             5957..>6663
                     /locus_tag="VI541_06110"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HLF70512.1"
                     /translation="MGIADEFERRLERLVEGVFNKAFKSSVQPAEIGRRIVREMERSK
                     SVSVGAVYVPNLFSISLSPTDHARLEGLFPSLSLEFAELVRGTAREKRWKPTGAIVIE
                     FQADPAVAEGRFDLTATHGSAVQEEAVPSPRTIAVVSVEGSAPAQNFELTGDAITIGR
                     STSSGIALSDPNASRDHAQLSFKEDAWWLTDLGSTNGTLVNDTLIKERRLTSGDRIRI
                     GSTILTFHEGVAGKTDA"
BASE COUNT         1285 a         2002 c         2091 g         1285 t
ORIGIN      
        1 aaaccgatgc ctacatgcgt gaagcgcggc tccatgacgt tctcacgatg gcttggactc
       61 tgcatgaaga ggtcatggat ccgctggacg ctgccgccca tcgcgacgtt ctcgccgagc
      121 aggcgagcac ccggcatgtt cgagcgggcc tgcgcgaagt atgagtcgtc gtgggagatg
      181 tcagcatccg ccctcatctt ctcggaccac gcgtgagcga tcgatgccgc ccatgagttg
      241 acggagagtg ccgacagccc ggcgccagtt cgctcaccat tgacgaggtc gaggagttgg
      301 ctctcgagcg atgcgatgga aggggcgcat gaggcatcgt tgggagggca ctcctgagca
      361 atggccggga tagaagttac aaggatcacg gcagccgaga tgacggtgag aaagagaaga
      421 tggcgacgtt gaacagacaa atggtcccct cgatctgtcc gtctcggtcg agatccggac
      481 ccttgatgta tcggccccca gacgcgtttg cagcaggcgc gtatacgtca ttcgtcggtg
      541 gtcacttggg gctagtcgcg catagtcact agtgactaga aggtcacgcg tttgttccgt
      601 gacgaatcgg aagagagagg acttatcggc gcggagggcc ggggactcga accccgaaga
      661 cctttcggtc ggcgactttc aaggccgctg cgatgccggt tacgcgagcc ctccatcgac
      721 agtctaaacc gcccatctcg ggcactagac cctcggtata cggtgggtca gcccgcaaac
      781 tttcttagag tgagtcgaag ccggtgtatg ggcgaagcgc ggcggggagt agcaccgacc
      841 cgtctgcctg ctggtgattc tcgaggatcg caacgatcgt gcgctgcact gcgacggccg
      901 tcccattgag cgtgtggacg aactcggccc ccgtcgcccc ttttgcgcgg atcccgagtc
      961 ggcgagcttg gtagtcgatg tcgttcgagc acgaggtcac ctcgagccag cgctgcgtgc
     1021 ccgggagcca cgcctcgata tcgaacttgc gatagttcgg atcggccagg tcgcccgcgc
     1081 agatctcgat gacccgatag gggatctcga gggcctggaa gatcgactcc tcgaggccca
     1141 ctagcatcag gtgctcacca tcggaatgct cggcacgaca gaacgagaac atctcgacct
     1201 tgtcgaactg gtggagcctg attatgccgc gcgtgtcttt gccgtaagtg ccggcctcgc
     1261 gccggaagca ggtcgagaac cccgcgtatc gcaggggaag tttgtcggca tcgaatgtct
     1321 cgcccccatg catcgatgcg aggggcacct cggcggtacc cgccaggtag aggtcgtcgg
     1381 cctccgtctt gtagacctgc tgttcgtcgg ttgggaagaa gcccgtcccg tacatcgctt
     1441 cttcacgcac cagcaccggc ggtatcaccg ggatgaagcc gtgcgccatc agccgatcca
     1501 tcaccagtcg tacgagcgcg aattcgagaa gggctccctt ccccttcagc atcccgaaac
     1561 gcgagcccga gaccttcgcc gcgcgctcga catcgatgat gtctaacgat tcaccgatgg
     1621 tcagatgatc tcgaccctcg aagtcgaatg tcttaacctc gccgaccgtc cttacgagca
     1681 ggttgctctc atcacccgaa ccgggggggg cctggggata gacgaggttc ggcatcgtgg
     1741 ccgcggcagc gtccaactct tcgcgcgcct tctggagttg agcctcggcc cccttgaacc
     1801 gttcggcgag ttcgcgcgcg gccgcgatcg cggattcgcg ctggtcgggg gccgtctgcg
     1861 cgatctgtcg gtttgcctcg ttgtgggccg cacggtcctt ctcgacagag gccgtcaact
     1921 cacgacatcg cgcctccaac tcgaccaggg catcgatgtc gatctttgaa ccgcgaagct
     1981 ctaacgaagc ccgaacgcgc tcgaggtcac ttcgaagaag tcgcagatcg atcaagtaac
     2041 ctccccacct tctgcttgcg cccgagcgct tcctggatag ctcgtcgttc ctcgtcggac
     2101 agattgtgtt cggaatcccc gttcagtagc accttggcgt agacccgcga ctcgctccgc
     2161 gcgttgatcg acgtgatcac gacaccgcta gcgtggtcat cgaggagcgc ggctgagaac
     2221 gacatcttgc cgcccatgtc ctcgaaggcg tcatatcgaa ccacgccgag gttgcgcgcg
     2281 gaacgtccga gtatggcgaa cacctcctcg atacgacgcg cctcgtgggc aaggtctccc
     2341 cggatcgtgt cgacgcgctc gaggtaggtc gagaccgcgt cgaggatcgt cttaccgtca
     2401 taagtatctt gaagcaggag gagggcccgg cgagccgcgc tgaatcgcct atgggctgag
     2461 atcgcgacga caaggctgag cattgcaaat gccatcgcca cgtatacgag caacctggtg
     2521 tccaaggacc gtgactcctc tcgggggcca tacaatatct gacctgatcg atctctcgga
     2581 gggacgcgtg gacgaagcat cgatacggct gctcttcgag aaggttcgaa gcggcgaaac
     2641 ctcgatcgat gacgctacgg cctcactcgc tggtcttcca ttctcggatc tcggctacgc
     2701 catgctcgac catcacaggg agctgcggac cgggctagcg gagtcggtct acgggccagg
     2761 caagacccca gatcaagtag cgggcatcgc tcaggatctg catgctcaga ggggaggggc
     2821 cgtgctcgtc acgcatgcat cggtcgaaca gttccgggcc acccgggagc gcgtgccgga
     2881 gtgcaccttc gacgagctca ccgggatgct cgtggtacgg cgctcggggg ccgaaccagc
     2941 cggggtcgta gtggtcgctt cagcgggaac gtccgaccag aaggtcgcac gcgagtgcct
     3001 catcactctc gatgctctcg ctatcaaagg ggaggccatc gaagatgtcg gagttgcggg
     3061 gatacatcga ctcttcgcat cgtcggaagc cctcgatcgt gcggacgtcg tcatcgccat
     3121 cgcagggatg gagggggcgc tcgccagcat catcgggggc ctcgtgagtg ttcctgtgat
     3181 cgcggtcccc acctcggtag gttatggcgc tggagcaggc gggatagccg ccctcctcac
     3241 catgatcaac tcgtgcgccc ccggtgttgc ggtcgtgaac atcgacaacg gctacggtgc
     3301 tgcggtcttc gccgcaacca tccttcgagc gcgttcatga tcgcctacct cgactgtttc
     3361 tctggagctg ccggcgacat gatcctggcc gcactcatcg atgcgggggc cgacgcatca
     3421 tcggttcgca agtctgtcga cctgctcggg ttgggggcct cgatcgagtt cgaagatgta
     3481 tcgcgcgcgg gcatccgtgg ggtccacacc agagtcacgg gagatgactc actcgcgccg
     3541 agaacattcg cagaggcccg cgcccttatc gaccacgccg agttgggtga ctcggttcgg
     3601 gcaaggtcac tctcgatcct cacggcactc gctcgagccg aggctaaggt ccatggcgta
     3661 accatcgagc aggtgcactt ccacgagata ggagccctgg acacactcat cgacatcatc
     3721 ggggtatcga gcgcaatgtc agagcttggt ctcgaaggct tcatggtcgg gccgatcgcg
     3781 acgagcatcg gttccatcga gtcgcagcat gggatcttgc ccctcccggc tccggcggtg
     3841 acggccctcc tcgaagggtt cgagctcgtc ggaagggcgg gcacccaaga gttagtaaca
     3901 cccaccgggg ccgccatcat cgcggcgact ggaacaccgg cccgcacctt cccaagcatg
     3961 aagatcgtca cgacggggta tggggctggc gcacgtgacc ttgagactcc caacctactt
     4021 cgcgtgatca tcggagaagc gcgctccgtc catgccgatg aggatgctgt gcagatcgag
     4081 gccaacatcg atgacatgaa ccccgagtac ttcgggtacg tattcgaaag gctcttcgca
     4141 gcaggggccc ttgatgtctg gacgagcccg atcgttggaa agaaagggcg gcccgccaac
     4201 atcatcggca tccttggacc agtctcagct gagatggctc tgcgtgacgt ggtgcttaca
     4261 gagacctcgt cgatcggcat ccgcgtcaca ccggtcagac ggtggatcct cgaccgcgag
     4321 tgggtgcagg ttacagtcga aggcatggcg gtacgggtaa aggtcgcgcg agatgggggc
     4381 cgggtggtga atatctcacc cgaatactcc gactgcgaag cggtcgcgcg cgaaaccggc
     4441 atccctctca aagaggtgtt cagactagcc ctaggttcat tgcgactcta gttagtacga
     4501 acctactgga ctatagggcc ccgcggcgcc gctatgaagg tgatggtgtt actgaagttt
     4561 gtgccttggg tcccaccggt atctatggct atcgcgttca ccttgtaaac accatagtta
     4621 agaaaaccgt tattgagact ttgagtgaga tacgagtaga gcgacttatg ggttacgcgc
     4681 cagtggacgt gggtatagtt gccggtcccg gggcaatcat cacaagtcgc gatgaggaac
     4741 ggctccaggt gcagtcctga ggcgtttcgg aagtagaccc tgatctggct tatcccggtg
     4801 ttgtcccagg cctcgccaac gatcgtgggt tcattcagaa agatcgtccc gtcggcagtc
     4861 gtaaccagcg cgccgggggg gctgatgtcg ttattcgcca gcacagagaa ggtccgtgtg
     4921 ttcgaggaac cactctggag agaggtgttc ggttgttcgg cgtcgaaggc ccagtaggcc
     4981 ttgccgaaga cggcgtagtt tccggcgggc atggcgacct gcacgctgaa cgtgccgcct
     5041 cccctgacca ggactcgagt cacgggcgcc gacgtgcccg cattgaagat gatcaccatc
     5101 gaccagggcg ttccggtacc cgtgaagacc accttgtcgg ggcgggtagt tgaagagccc
     5161 tcggcaggac ccgtgatggt tggagtcgca ggaggcgctg agggagagac gtcggtcact
     5221 actccgcaca tcccgtgaca aaccccttcg tacccaggga aatccgaatc ggcccagctg
     5281 tggttggtac acctatagaa gatgatcccg ccgttgtagg tggctgtgta cgagggattg
     5341 gtctggctca gagttggcga gttgaagtct gtgtagctgc cgctccccgt ccaaccggtc
     5401 accgtgtgct caggtggagt ctggaaggtc gggttccagt tccaagtcac ggtgtctcct
     5461 gcagaggcgc tcaggacatt cgggctgaag gagtttgagt tgttgggccc tgcctgcacc
     5521 gtgcgcggcg tcgccgaagc caaaccggga acgaacatcg ccacaagcgt ggcgcaggct
     5581 acgaatacac cgaggatgga tcttgcgtgt ctgctcatca gccgccccct cactcaacga
     5641 ctacgcgaac ccgcctgatg gttgcgtcga tgagtgcgat ggagcgggta tatctcccta
     5701 gaagcaccca accgccggtc gggtacctct tggctcttca tgttagcacc gcgcccatgc
     5761 tcggggggcc agacacctgt gcgcgagaga ggacttgaac ctccacgagc ttgcgcccac
     5821 tggcacctga agccagcgcg tctaccattc cgccactcgc gcttgattgc gagggaatct
     5881 tagccgcccc tggcatcgtt gcgccgcgag cccactggcg tgggatattt acagccgcga
     5941 accgcaagga gtgagcgtgg gcatagccga cgagttcgaa aggcgcttgg agagactggt
     6001 cgagggggtc ttcaacaagg ccttcaagtc ctcggtccag cccgccgaga tcggtcggcg
     6061 gattgtaaga gagatggagc gctccaagtc cgtgagcgtg ggcgcggtct atgtccccaa
     6121 cctcttttct atctcgctct cacccaccga tcatgctcgg ctcgaggggc tcttcccaag
     6181 cttgagcctg gagttcgcag agttggtccg aggaactgcc cgcgagaagc ggtggaagcc
     6241 taccggggcc atcgtcatcg agttccaggc agatcccgct gtcgccgaag gacgattcga
     6301 cttgactgca acacacggat ccgcggtcca ggaagaggcg gtcccctcac cacgaaccat
     6361 cgccgttgta tccgtcgagg gttcggcccc cgctcagaac tttgagctga cgggtgacgc
     6421 gatcaccatc ggccgctcga cctcatcggg catagcgctg agcgacccca acgcgtctcg
     6481 agaccacgct cagttgtcgt tcaaagaaga tgcttggtgg ctcacagatc ttggatcaac
     6541 gaacgggaca cttgtgaacg acacgctcat caaggagaga cgactcacat cgggggatcg
     6601 gatacggatc ggcagcacga tcctgacctt tcacgaaggg gtcgcaggga agaccgatgc
     6661 ctg
//
LOCUS       DATDOZ010000304         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3643421, whole genome shotgun sequence.
ACCESSION   DATDOZ010000304 DATDOZ010000000
VERSION     DATDOZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643421"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VI541_06115"
     CDS             complement(<1..268)
                     /locus_tag="VI541_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70513.1"
                     /translation="MSLSVLVGVLPGWVGARADHPQWVTSFPVNSATDPAKGGTPSVL
                     LERGHTYRIIVSGTYVYHTMTNSPFLADAECSIDLTDSQWVREGY"
     gene            406..957
                     /locus_tag="VI541_06120"
     CDS             406..957
                     /locus_tag="VI541_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLF70514.1"
                     /translation="MRHFAVSVIGDDRPGIIAAVSGALVDRGCNIEDASSTILRGHFT
                     MVLVISAPEDLAEVTDQALETAIREATSQMGLIVAVRPIGEIQEAHKAPAEHLTSLSV
                     YGSDRPGIVHGFTSLLAGRGINIVDLSTRLVGQPGDEVYAMTLDLAVPSSADVDELMN
                     ALSALADQLQVEFSLHEADADIL"
     gene            964..1464
                     /gene="def"
                     /locus_tag="VI541_06125"
     CDS             964..1464
                     /gene="def"
                     /locus_tag="VI541_06125"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLF70515.1"
                     /translation="MPIRPVLRFPHGALKSPSSDITAIDEDALSLAQDLIDTMRSADH
                     CVGLAAPQIGINIRAFALNVRGHPKALSDHGEFVLFNPRIIEGYDAVEGREGCMSVPD
                     LTGDVMRWSRVTVAGMAIDGSQMTLEADAFEARAIQHEVDHLDGLLFLDRVASREALF
                     RRRTYR"
     gene            complement(1477..2646)
                     /locus_tag="VI541_06130"
     CDS             complement(1477..2646)
                     /locus_tag="VI541_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLF70516.1"
                     /translation="MRGPLEGVKVLAFEQAIALPFCSFIFAELGADVIKIEQPGRGDV
                     VRGWDRVVGGLSSGFVAFNAGKRDISVDVSKPDGQLIVSELAARADVFIENFAPGVVD
                     RLGLGHETLRGESPRLIYASLSGYGQDGPYRDVKAFDLLIQGEAGILMTNGYPEAPAK
                     VGMPITDLIGGSHVAMGVLAALYERERTGVGSYIDLALLDSAFFWSGYFPHYEWHGQA
                     PPPRSGMRHQFICPYGPYLASDGKYVNLVVASEKHWKIFCEKVVARPAWIEDPRFSSI
                     EARLVNREQLELAVEEVIATAPHDEWMRRAADAQLPYGEVRDMASVVGHPQLASRGMI
                     VRSSSPVGEIPLARFPLSTKTRRSVPEMGEHTEEILRELGYDLERIESLRRSGVI"
     gene            complement(2654..>2886)
                     /locus_tag="VI541_06135"
     CDS             complement(2654..>2886)
                     /locus_tag="VI541_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70517.1"
                     /translation="GRLNGVWGLREFAVPVHVARNLTPRIKKLWWYPYDDETVRTLRG
                     VAELFSAPSWSEKKQALRTILTNVVPAIRNKY"
BASE COUNT          567 a          910 c          876 g          533 t
ORIGIN      
        1 ggtacccctc tcgtacccac tgactatcgg tcaggtcgat cgaacactcg gcatctgcca
       61 aaaatgggct gttggtcatc gtgtggtaga cgtatgtgcc agaaacgatg atgcgataag
      121 tgtgccctcg ttccagcaga accgatggag tcccaccctt cgcagggtcc gtggcgctgt
      181 tgactggaaa gctcgtcacc cattgcggat gatcagctcg cgcacccacc caacccggca
      241 agacaccgac gagcacgctc agggacatca ccactatcgt gggggcagca cggcgatcgc
      301 gaaagaacgg catgtctaga ccattcggca gagtcggcca tatccctgcc tggatccgag
      361 ccccatcggc cccccttagc ccccgagata ggcaagaatg aacctatgag gcacttcgcc
      421 gtcagcgtca taggtgacga tcggcccggc atcatcgcgg ccgtctcggg cgcgctcgtc
      481 gatcgagggt gcaacatcga ggacgcttcg agcacgatcc ttcgcggtca cttcaccatg
      541 gtgctggtca tctcggcccc cgaggacctc gccgaggtca ccgaccaagc actggaaacg
      601 gcgatcaggg aggccacatc gcagatgggt ttgatcgtgg ccgtccggcc catcggcgag
      661 atacaggaag cgcacaaggc ccccgccgaa catctcacct ctctatccgt ctacgggtca
      721 gaccgcccgg gcatcgttca tggtttcaca tctctgcttg cgggcagggg catcaacatc
      781 gtcgacctga gtacccggct cgtggggcaa cccggtgatg aggtgtatgc gatgaccctc
      841 gacctcgctg tgccatcgag cgccgacgtg gacgagttga tgaacgcact gagcgcactg
      901 gcggaccaac tgcaggtcga gttcagtctg cacgaggccg acgcggacat cctctaacgc
      961 taggtgccga tacgcccggt ccttcgattc ccgcacggcg cgctcaaatc tccgagcagc
     1021 gacatcaccg cgatcgatga ggatgcccta tccctggcgc aggatctcat cgacacgatg
     1081 cgctcggcgg accactgtgt cggcttagca gcaccgcaga tcggcatcaa catccgagca
     1141 ttcgctctca acgtccgagg gcaccccaag gccctctccg accacggcga gttcgtactc
     1201 ttcaatccca ggatcatcga agggtacgac gcggtcgagg gaagggaggg gtgcatgagt
     1261 gttccagatc tgacgggcga cgtcatgcgc tggtcgcgcg tgaccgttgc cgggatggcc
     1321 atcgatggtt cccagatgac gctcgaggcg gatgcattcg aggctcgcgc gatccaacat
     1381 gaggttgacc acctcgacgg gctgctcttc ctcgatcggg ttgcttcccg agaagctctg
     1441 ttccgcagac gcacctaccg ctgacctggg ccctttctag atgaccccgc tgcggcgaag
     1501 gctctcgatc cgttctaggt cgtagccgag ctctctcagg atctcttcgg tgtgttctcc
     1561 catctcgggg accgagcgcc gcgtcttggt cgatagagga aagcgcgcaa gcggtatctc
     1621 tccaacgggt gaagacgatc gaacgatcat cccgcgtgag gcaagctgag ggtggccaac
     1681 gaccgaagcc atatctcgta cctcgccgta tggcagctgc gcatcggcgg ccctgcgcat
     1741 ccactcatcg tggggggccg tggcgatgac ctcctcgacg gccaactcga gttgttcgcg
     1801 gttgaccaga cgggcctcga tggacgagaa gcgcggatcc tcgatccacg caggacgcgc
     1861 cacgaccttc tcgcagaaga tcttccaatg cttctcactc gcgacgacga ggttcacgta
     1921 cttgccgtcg gatgcgaggt aggggccgta aggacagatg aattggtggc gcatcccgct
     1981 gcgcggtggg ggggcctgac catgccactc gtagtgcggg aagtaaccag accagaagaa
     2041 ggccgaatcg aggagggcca ggtcgatgta ggaaccgacc ccggttcgct ctctctcgta
     2101 gagggcagca aggaccccca tggcgacatg cgagcctccg atgagatccg tgatgggcat
     2161 gccaaccttc gctggcgctt cgggatagcc gttcgtcatg aggatccctg cctctccctg
     2221 gatgagcaga tcgaaggcct tgacgtcgcg atagggcccg tcctgcccgt agcccgagag
     2281 gctggcgtag atgagacggg gcgactcgcc gcgcagggtt tcgtgaccga ggccgaggcg
     2341 atcgaccact ccgggggcga agttctcgat gaagacatca gccctcgccg caagctcgct
     2401 gacgatcagt tgaccgtcgg gtttgctcac atccaccgag atatcgcgtt tgccggcgtt
     2461 gaatgccacg aatcccgagg agaggccccc gactacgcgg tcccatccac gaaccacatc
     2521 gccgcggccc ggctgctcga tcttgatcac gtctgcaccg agttcggcga agatgaatga
     2581 acagaaggga agagcgatcg cctgctcgaa ggcgagcacc ttcacgccct caagtggccc
     2641 tctcaagtgc gtctcagtac ttgttcctga tcgctggtac gacgttcgtc aggatggtgc
     2701 gcaaggcctg cttcttctcg gaccagctgg gggccgagaa gagttcagct acaccgcgca
     2761 atgtgcggac ggtctcgtcg tcgtatgggt accaccatag tttcttgatc cggggcgtca
     2821 ggttgcgagc aacatgtacc gggacagcga actcacgtaa accccagaca ccgttcagcc
     2881 tgccgt
//
LOCUS       DATDOZ010000305         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U7907
            k141_3658251, whole genome shotgun sequence.
ACCESSION   DATDOZ010000305 DATDOZ010000000
VERSION     DATDOZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962676
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:19:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,231
            CDSs (total)                      :: 1,210
            Genes (coding)                    :: 1,200
            CDSs (with protein)               :: 1,200
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 10 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658251"
                     /isolate="SMAG_U7907"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="hisF"
                     /locus_tag="VI541_06140"
     CDS             <1..648
                     /gene="hisF"
                     /locus_tag="VI541_06140"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HLF70518.1"
                     /translation="ARYDAEGADEVVFLDITASHEGRETMLDVARRTAQEVFIPLTVG
                     GGVRTIADIRALLRAGADKVGVNTAAVDRPELISEGAAAFGAQCIVVAIDAKRVGDGW
                     DVYTHGGRTSTGLDAVAWARRAEELGAGEILLTSMDRDGTREGFDIELLRAVDDVVNL
                     PVIASGGAGKLEHFPEAVLEGKADAVLAASLFHFGELTVGEVKHAMAREGIAVRL"
     gene            complement(650..2227)
                     /locus_tag="VI541_06145"
     CDS             complement(650..2227)
                     /locus_tag="VI541_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLF70519.1"
                     /translation="MPDVIVIGCGAGGAVAAKELAEHGAEVLVLEAGPWYQDLDKDFN
                     RQQHDMASLVDGRFRWGPPDRSQPPWTRRRDGVWLMYQLAGVGGTTLHYNGISVRAYP
                     DAFERSGWPVSYEEMIPYYERVEAFLPVSQPTRLAYKDALFAQGCEAIGLSQKDSPDV
                     EDVCWRPSPNAILPIADLSKGLHWPEADGCTMCGHCLIGCSNPQGAPTHRKAKRATNV
                     SYVPAAVETGRCEIRPGSFVQRVLTRDGVATGVVWKDEAGGTHEAQAKVVVLAGGVVE
                     SPRLWLNSELPNSHDVVGRYFTHHLQDMVTGLFPQDVHQDVGQVTMARSDFPGYGCLF
                     SQGLEPQSFAIAITGAGRSPIRETPGEPWDTVGRAWGEESRRLHDSYSKALSVVIIAD
                     DEEHPDNRITIDEGFGPDANGPIPLMIYHPTAESTRRRDWLAAKAAETLRAAGATHIH
                     RTDHAPLVTHPMGTMRMGNDPKTSVVGPDGEAHEVERLFIADNSVFANGLGGPNPTLT
                     CQALATRTADRIIAKYL"
     gene            complement(2220..2606)
                     /locus_tag="VI541_06150"
     CDS             complement(2220..2606)
                     /locus_tag="VI541_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70520.1"
                     /translation="MSHDDDVLATLAAAVDTILPGSSGAGVQALVADLFDGVMEGFPM
                     MVSAILDAFASEISGGHPFAQLSAQQRGEVFETMMSDPSPDVNDVVDGLFLFTLGQNY
                     SEAHPDHDAIWERLGYHGPSEGIVDA"
     gene            complement(2647..2811)
                     /locus_tag="VI541_06155"
     CDS             complement(2647..2811)
                     /locus_tag="VI541_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF70521.1"
                     /translation="MSLEVSGGITLKNVEKIARLKPDAISVGRITHSAPAVDLALHVL
                     GPAGAGPRTD"
BASE COUNT          524 a          851 c          910 g          536 t
ORIGIN      
        1 gcccgatacg atgccgaagg agccgatgaa gtcgtattcc tcgatatcac ggcttcgcat
       61 gagggtcgtg agactatgct cgatgtggcc cgacgtacgg cccaggaagt tttcatccca
      121 ttgaccgtcg gtgggggagt gcgcacgatc gcagacatca gggctcttct aagggcgggc
      181 gcggacaagg tcggagtgaa caccgccgcc gtggatcgcc cggagctgat ctcagaggga
      241 gcggccgctt tcggggctca atgcatcgtg gttgctatcg acgcgaagcg agttggcgac
      301 ggatgggatg tatacaccca cggaggacgt acctccacgg ggttggatgc ggttgcctgg
      361 gcgcgacgtg ccgaggagtt gggggccggc gagatccttc taacctcgat ggatcgcgat
      421 ggcacccggg aaggcttcga catagaactg ctaagagcgg tggatgatgt ggtcaacctt
      481 ccggtgatag cttccggggg ggccgggaaa ctggagcact tcccagaagc ggtcctcgaa
      541 ggcaaagccg atgctgtgct ggcggcgtcg ctcttccact tcggcgaact gaccgtcggc
      601 gaagttaagc acgcgatggc ccgagagggg atcgcagtaa ggctctgatt cagaggtact
      661 tcgcgatgat ccgatcagct gtgcgcgtag cgagggcctg acaggtaagc gttgggttcg
      721 gcccccctag tccgtttgcg aataccgagt tgtccgcgat gaagagccgt tcgacttcgt
      781 gtgcctcgcc gtcgggacca accaccgagg tcttcgggtc gttgcccatc cgcatcgttc
      841 ccatgggatg tgtcacgagg ggtgcatggt cggttcggtg gatatgagtc gcaccagcag
      901 ccctcagagt ctctgcggct ttggcggcga gccagtctcg gcgtcgcgtc gactcggcgg
      961 ttgggtgata gatcatcaag gggatagggc cattcgcatc ggggccgaaa ccttcgtcga
     1021 tcgtaatgcg attgtcgggg tgctcttcgt cgtccgcgat gatgacaacc gatagggctt
     1081 ttgagtatga gtcgtgcagg cgtcgcgact cctctcccca agcgcggcca accgtgtccc
     1141 agggctcgcc aggcgtttcg cggatggggg aacgcccggc ccccgtgatc gcgatggcga
     1201 atgactgtgg ctcgaggccc tgcgagaaga ggcatccgta accggggaag tccgaccgtg
     1261 ccatcgtgac ctggcctacg tcctggtgga cgtcttgtgg gaacaagccc gtaaccatgt
     1321 cctgaaggtg gtgggtgaaa tatctgccga cgacatcgtg tgagttgggc agctcggagt
     1381 tgagccacaa gcgcggactc tccacgacgc ctccggcgag gacgaccacc ttggcttgag
     1441 cttcgtgagt gccaccggcc tcatctttcc aaacgacacc cgttgcgacc ccgtcgcggg
     1501 tcagaaccct ctggacgaaa gaaccgggcc ggatctcgca acggcccgtc tcaaccgctg
     1561 cggggacgta cgagacgtta gtcgcgcgct tcgccttgcg gtgcgtcggg gccccctgag
     1621 ggttcgaaca tccgatcaag cagtggccgc acatggtgca gccgtcggcc tcgggccagt
     1681 gcaaaccctt cgacagatcc gcgatgggaa ggatcgcatt cggcgagggc cgccagcaca
     1741 cgtcctccac gtccggcgag tccttctgcg agagaccgat ggcttcacac ccctgggcga
     1801 acagagcatc tttgtatgcg agtcgggtgg gctgcgagac ggggaggaag gcctcgacgc
     1861 gctcgtagta cgggatcatt tcctcataag aaacgggcca tccgctccgt tcgaaggcat
     1921 ccggatacgc acgcaccgag atgccgttgt agtgcagagt tgtccccccg actccagcca
     1981 gctgatacat gagccagaca ccgtcgcgtc gtcgcgtcca cggaggctgg cttcggtctg
     2041 gggggcccca gcgaaacctc ccgtcgacga gcgaggccat gtcgtgctgc tgtcgattga
     2101 agtccttgtc gaggtcttgg taccagggcc ccgcttcgag aaccaggacc tcggccccgt
     2161 gttccgccag ctccttggct gcaactgctc cgccagcacc acacccgatg acgatcacat
     2221 caggcatcga cgatcccctc tgaaggaccg tgatatccga gtcgttccca gatcgcgtca
     2281 tggtcggggt gagcctccga gtagttctgg cccagcgtga acaggaacag accatcgacc
     2341 acgtcgttaa catcgggtga agggtcgctc atcatcgtct cgaagacctc tccacgctgt
     2401 tgggcagaga gctgagcgaa tggatggccc cccgatatct ccgaggcgaa tgcgtcgagg
     2461 atggccgaga ccatcatcgg gaaaccctcc atgactccgt cgaagagatc tgccaccaac
     2521 gcctgtaccc cggcccccga agacccgggc aggatcgtgt cgacagcggc cgcgagcgtt
     2581 gcgagtacat catcatcgtg ggacatgctt cctcctgcgc ggaatcctat gtcaggtgct
     2641 accgcgttag tcggtgcgag gtccggcccc cgccggtcca agtacatgaa gagccaagtc
     2701 gacggcgggg gccgagtgag ttatccgtcc caccgatatc gcgtcgggct tgagtcgggc
     2761 aatcttctcg acattcttca gcgtgatccc acccgacacc tccagtgata tccggcccct
     2821 g
//
