LOCUS       DATDPQ010000001         3415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_784817, whole genome shotgun sequence.
ACCESSION   DATDPQ010000001 DATDPQ010000000
VERSION     DATDPQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784817"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VI520_00005"
     CDS             complement(<1..617)
                     /locus_tag="VI520_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF36293.1"
                     /translation="MQRKNLIAGLAVLAVMLAACQTAAEPAAGEVEVFSWWTGGGEAA
                     GLDAMLAVFTTEYPDVVFTNSAVAGGAGTNARAVLASRLQAGDPPDSWQAHAGQEIIG
                     TYVAAGQIESLNSLFEDEGWIAVMPDTLIPLISKDGEIYSVPVNIHRANVMWTNPTLL
                     SDLGVTVPTTWDQFFAACETIEAAGKICLSMGEQWTAMHLMEVVLL"
     gene            821..2590
                     /locus_tag="VI520_00010"
     CDS             821..2590
                     /locus_tag="VI520_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36294.1"
                     /translation="MALREFSLANEHSYNRSGPVRWIASHVLRYPIWPAAMVLAAVVN
                     NTAFSYVQLFVGRAFDVIAAPGFTRAALGGAAAAVFLAAGIQGLSGLLRNYAGEFLAQ
                     GIERDARHELYVSLLGKSQTYHRRQRIGDLMARATNDVHSLNFMFSPGIMLITDSVMN
                     LIVPLVLIGALLPELLLVPLVFTAVLVVTLRDYARRLGPVTVGMRMQFGVMNAGLAET
                     IAGIEVVKANVQEQHEWRKFTHNAGRYRELFVQHGMIQARYWPMLAFSLFWAVGFFHS
                     LLVWQAGRITLGEVVAYVGLFGGLRFPTFISLFSFDLVQLGLAGARRVLEVLNARTEL
                     DENLGGVARPIRGEVAFHNVTFGYNGNPVLKNLNFTAQAGETLAIVGQTGSGKSSLTH
                     LINRIYDASEGRVEVDGVDVREWSLESLRSQIATIEQDIFLFSGPIRDNIAFAREQAT
                     DEQIELAAREAQAEEFIRGFPGGYQTEIGERGVTLSGGQRQRLAIARAFLSNPRILIL
                     DDSTSAIDSATEDQIQRAMRRISRERTTFLITHRLSQIRWADRILVLRRGELVGQGTH
                     EQLMQESPDYRRIFDRFGEANGE"
     gene            2597..>3415
                     /locus_tag="VI520_00015"
     CDS             2597..>3415
                     /locus_tag="VI520_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36295.1"
                     /translation="MGFILDGLETESYDREYRDRDLVRRILIYFLEHSRPIGLVVLAL
                     TVNSAAGAGAPIAIARVIDAVGSDPSLRTIAIGGAVVFGMGGLAWAANFIQQWVSARV
                     IGDVVLKLRADVLAATIRHDLSFFDLHPTGKIVSRATSDTQDFSDVVSLVISLISQVL
                     LLAILVVWLLAVNAALTLQLVAMAPVAVVLALSFRRVARRVTQHARRVTAAINAQIQE
                     SISGIGVAKAFRKERSIYETFDGNNRQAYQIGLRRGLTLVTIFPVIGVSTGLGMA"
BASE COUNT          593 a         1098 c         1095 g          629 t
ORIGIN      
        1 agcaacacga cctccatcaa atgcatggcc gtccactgct ctcccatcga gaggcagatc
       61 ttgccggcgg cctcgatcgt ctcacaggcc gcgaagaact gatcccaggt tgtcggcacc
      121 gtcacaccca gatccgacaa cagggtcggg ttggtccaca tcacgttggc gcggtggatg
      181 ttgaccggga ccgagtagat ctccccgtcc ttcgagatca gcgggatgag ggtgtcgggc
      241 atgaccgcga tccagccttc gtcctcgaac aggctgttga gggactcgat ctgcccggcg
      301 gcgacgtagg tcccgatgat ttcctgaccg gcgtgggcct gccaggagtc gggcgggtct
      361 ccggcctgca ggcggctggc gagcacggct cgggcgttcg tgccggcgcc gccagcgacg
      421 gcggagttgg tgaacacgac atccggatac tcagtagtga agaccgccaa catggcgtcc
      481 aggcctgccg cttccccgcc gccggtccac cagctaaaca cttccacttc accggcagct
      541 ggctcggccg cggtctggca agccgccagc atcactgcga gcacagccaa tccggcaatc
      601 aaattcttcc tctgcatttc agctttcctc ctcaggatga ttggcccggc aaatggtgac
      661 cgggtgattg acgtgggcga ggggtccggc ctcgagacgg gcgtatcctc ctcattaccc
      721 gaaaccttcg aagtataacc catgcggcgc ggatccttgt cagacaacag cggcagcccg
      781 accgtataat gtggaccgac tcagccaacc caagactacc gtggcccttc gcgaattctc
      841 cctagctaac gaacacagct acaaccgctc cggtccggtg cgctggatcg cctcgcatgt
      901 gctgcgctac ccgatctggc cggcggcgat ggtgctggcg gcagtggtca acaacacggc
      961 cttcagttac gtgcagctgt tcgtcggacg ggccttcgat gtgattgccg cccccggctt
     1021 cacccgggcg gcccttggcg gcgcggcggc agccgtgttt ctcgcggccg gcatccaagg
     1081 gctgtcgggc ctgctgcgca actatgccgg tgagttcctg gcccagggaa tcgagcgcga
     1141 cgcccggcac gagctgtacg tcagcttgtt gggcaagagc cagacctacc atcgccgcca
     1201 gcggatcggc gacctcatgg cccgcgccac caacgacgtc cactcgctca atttcatgtt
     1261 cagcccgggc atcatgctga tcaccgactc ggtgatgaac ctgatcgtgc cgctggtact
     1321 gatcggcgcg ctgctgcccg agctgctgct ggttccgctg gtcttcactg cggtgctggt
     1381 tgtcacgctg cgagattacg cccggcggtt gggaccggtg acggtcggaa tgcggatgca
     1441 attcggggtg atgaacgccg ggctggccga gacgattgct gggatcgagg tagtgaaggc
     1501 caacgtgcag gagcagcacg agtggcgcaa gttcacccac aatgccggcc gctaccgaga
     1561 gctgttcgtg cagcacggga tgatccaggc ccgctactgg ccgatgctgg cattcagcct
     1621 gttctgggcg gtggggttct tccactcgct gctggtctgg caggccggac gcatcacatt
     1681 gggtgaggtc gtggcctacg taggtctctt cggcggtctg cgtttcccga ccttcatctc
     1741 gctgttctcc ttcgacctgg tgcagctcgg gctggccgga gcccgccggg tcttggaggt
     1801 gctcaacgcc aggaccgagc tggacgaaaa cctcggtggc gtggcccggc cgatccgggg
     1861 tgaagtggcc ttccacaacg tcaccttcgg ctataacgga aatccggttc tcaagaacct
     1921 caatttcaca gcccaggccg gcgagaccct cgccatcgtc ggccagaccg ggtcggggaa
     1981 atccagcctc acccacctga tcaaccggat ctatgacgcc agcgagggcc gggtcgaagt
     2041 ggatggagtg gacgtgcggg agtggagcct cgagtcgctg cggtcccaga tcgccaccat
     2101 cgagcaggat atctttttgt tctccggccc gatccgagac aacatcgcct tcgcccggga
     2161 gcaggccacc gacgagcaga tcgagctggc cgcccgcgag gcccaggcag aagaattcat
     2221 ccgcggcttc ccaggcgggt atcagaccga gatcggggag cggggggtta ccctttccgg
     2281 cgggcagcgc cagcgcttgg ccatcgcccg ggccttcttg agcaatccgc ggatcctgat
     2341 cctggacgac agcaccagcg ccattgacag cgccactgag gatcagatcc aacgcgccat
     2401 gcgacgaatc agccgcgagc gcacgacatt cctcatcacc caccggctga gccagatccg
     2461 ctgggcggac cggatcctgg tgctccgccg tggcgagctg gtcgggcagg gaacccacga
     2521 gcagctcatg caggagagcc cagattaccg gcggatcttc gaccgtttcg gagaggccaa
     2581 cggcgagtaa accaacatgg gattcatact cgacggtcta gagaccgaat cctacgaccg
     2641 ggaataccgc gaccgagacc tggtacggcg catcctgatt tacttcctag agcacagccg
     2701 tccgatcggg ctggttgttc tggcgctgac cgtgaattcg gccgccggtg ccggggctcc
     2761 gatcgcaatc gcccgggtga ttgatgcggt cgggtcggac ccgtccctgc ggacgatcgc
     2821 gatcgggggc gcggtggtgt tcgggatggg cggcctcgcc tgggcagcca atttcattca
     2881 acagtgggtg tcggcccggg tgatcgggga tgtcgtgctc aagctgcggg cggacgtgct
     2941 ggcggcgacc atccgccacg acttgagttt cttcgacctg caccccaccg gcaagatcgt
     3001 cagccgggcc acctccgaca ctcaggactt ctcggatgtc gtctccttgg tgattagcct
     3061 gatcagccag gtgctgttgc tggcgatcct ggtggtctgg ctgctcgcgg tcaatgccgc
     3121 attgaccctg cagctggtcg ccatggcacc ggtggcggtc gtgctggccc tgagcttccg
     3181 acgggtggcg cggcgggtca cccagcacgc ccggcgggtg accgccgcga tcaacgccca
     3241 gatccaagaa tcgatcagcg ggatcggggt ggcaaaagcc ttccgcaagg agcgctcgat
     3301 ttacgagacc ttcgacggca acaaccgcca ggcttatcaa atcgggctgc ggcgcggtct
     3361 cactctagtc acgatcttcc cggttatcgg agtctcgacc ggattgggga tggcc
//
LOCUS       DATDPQ010000002         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3662897, whole genome shotgun sequence.
ACCESSION   DATDPQ010000002 DATDPQ010000000
VERSION     DATDPQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3662897"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VI520_00020"
     CDS             complement(<1..268)
                     /locus_tag="VI520_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36296.1"
                     /translation="MNRSIFVSLLALAALAACRPQPEVPASPTVEPNFVEAETLDTLR
                     SAAIPMADPEDLARRLSGVTQDIPATLEPPAAPRKVAARDRFWIT"
     gene            complement(284..>2404)
                     /locus_tag="VI520_00025"
     CDS             complement(284..>2404)
                     /locus_tag="VI520_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HLF36297.1"
                     /translation="ANLIGRQVLRVAGRGEARLVAAIMLTAGLMSSVLNNVGVAALLL
                     PVVMDLARRTGRNPSRLLMPLAFGALLGGVTTLIGTPPNILVNDVLADYGLTRFEFFD
                     YTPVGVAVLLAGVLFMVLIGRHLLPERDITKESARADLGQIFGYRERMAILRLPADSS
                     LSGHTLAASRLGAALGLNVLAILRPQDRLLAPGPNAVLRAGDQLIVEGLLTRLEELQA
                     RDNLQLDDRPVDLTELVSAEAEFAEVQVAANSALVGQSLAQIGFRHQFGVNILAIRHD
                     GQLRRSDLQERPLYVGDALLIHATQVQLKFLAELPDFVTVSQLSPPEVEKEYRLRETL
                     LSLVIPPESILAGRTLAESRLGDAFGLSVLGIRRNGKTQLAPHPEDRLEAGDQLLVEG
                     RPEDLELLRGLRELIVDRESPIDLTQLESDLVGLAGVVLSPQSTLNGKTLRELHFREK
                     YGLSVLAIWHSGTPRRSNLRDIPLRFGGALLLYGSRQKLKVLGREPDFLVLTEEAQEP
                     PRLGKAPVAALVMLGVFAPVILGWIPIAIAAIVGAALMVLLGCLTMEEAYRDIEWRAV
                     FLIAGMLPLGIAMQQTGAAAYLADQLVGLIGSPGPLGVIAGLFLFTALITQVMPTPAV
                     VVLIVPIAISTAAAMDISPYAMTMVVAVSAAGCSFNPVSHPANSLVMGPGGYRFSDFL
                     KVGIPLTAVILAVTLLVLPVFWPL"
BASE COUNT          497 a          778 c          740 g          389 t
ORIGIN      
        1 tggtgatcca gaacctgtcc cgggcggcga ccttcctcgg cgcggccggc ggctccaggg
       61 ttgcggggat gtcttgggtc acgccgctca gccggcgggc caagtcctcc ggatcggcca
      121 tggggatcgc cgccgatcga agggtgtcaa gcgtctcggc ctcgacgaaa ttcggttcga
      181 cggttgggct tgcagggact tcgggctgag ggcggcaggc cgcgagcgcg gcgagggcga
      241 gcaggctgac aaagatggag cggttcatgc agcccttcat aacttaaagg ggccagaaca
      301 ccggaagcac gagcagcgta acggcaagaa tgactgccgt cagcgggatt ccgactttga
      361 gaaaatcgct gaatcggtag ccccctggcc ccatcaccag gctgttggct gggtgcgaaa
      421 ccgggttgaa tgagcagcct gcggcagaga cggcgaccac catggtcatt gcatagggtg
      481 agatgtccat ggccgcagcc gtactgatgg cgatggggac aatgagaact acgaccgccg
      541 gggtaggcat cacctgagtg attagggcag taaagagaaa cagcccggcg ataaccccga
      601 ggggtcccgg cgacccgatg agcccgacca attggtctgc caggtaagca gctgcgccgg
      661 tctgctgcat ggcgatccca agcggcaaca tcccggcgat caaaaacacc gcccgccact
      721 cgatatcccg ataggcttcc tccatcgtga ggcacccgag cagcaccatc aaggctgcac
      781 cgacaatcgc cgcgatcgca atcgggatcc atcccaggat gactggcgca aacaccccga
      841 gcatcaccag cgcggccacc ggcgccttgc caagccgcgg cggctcctgg gcctcttcgg
      901 tcagcaccag gaagtcaggc tctctgccca gaactttgag cttctgtcgc gagccgtaga
      961 gcagcagcgc gccaccgaat cggagaggga tgtcccgcag attcgagcgg cgaggggtcc
     1021 cgctgtgcca gatggccagc acgctcaagc cgtacttctc ccggaagtgc agctcacgca
     1081 atgtcttgcc attcagagtc gattggggcg aaagcaccac gccggccagc cccaccaggt
     1141 ccgattccag ctgggtcaag tcgatcggcg actcccgatc cacgatcagc tcccgcaacc
     1201 cgcgcagcag ttcaaggtcc tccggccgac cttccaccag caattgatcc cccgcctcta
     1261 accggtcttc cgggtgcggt gcgagctggg tcttcccgtt tcgcctgatc cccaataccg
     1321 agaggccaaa agcgtcgccc aatcggctct cggccaaggt ccggcccgcc aggatggact
     1381 cgggcgggat gacaagcgat agcagcgtct cgcggagccg atattccttc tcaacctccg
     1441 gaggtgaaag ctgggagacg gtgacgaagt ctggcagctc agctaggaac ttcaactgca
     1501 cttgggtcgc gtgaatcagt agggcatctc cgacatacag cggacgttcc tgcaagtcgg
     1561 atcttcggag ctgcccatcg tggcgaatgg ccagaatgtt caccccgaat tgatgacgga
     1621 aaccgatctg cgccaggctt tggccgacca gcgccgagtt ggccgcgacc tgaacttcgg
     1681 caaactcggc ctcggctgac acgagctcgg tcaggtctac cggccgatcg tccaattgaa
     1741 gattatcccg cgcttgcagc tcctccaatc gggtgagcag cccctcgacg atcaactgat
     1801 cgcctgcccg cagtacagca tttggaccgg gagccagcag tcgatcctgg gggcgcagga
     1861 tcgccaacac gttcaatccc aacgcggcgc ccagtcggct ggctgcgagc gtgtggccgg
     1921 acagcgatga gtcggctggc aggcgcagga tcgccatccg ctcccgatag ccgaagatct
     1981 gaccgaggtc ggcccgggcc gattccttcg tgatgtcacg ctcgggcaac aagtgccttc
     2041 cgatcaacac catgaacaaa actccggcca gcagcactgc cacgccaacc ggtgtgtagt
     2101 cgaagaactc aaagcgggtc agcccgtaat cggccagtac gtcattgacc aggatgtttg
     2161 gcggagtccc aatcaatgtg gtcaccccgc ccagcagcgc cccgaaggcc aaaggcatca
     2221 agagccgaga cggattgcgt ccggttcgcc gggcaagatc catgacgacc ggcagcaata
     2281 gggcggcaac tcccacgttg ttcaagaccg atgacatcag cccggcggtg agcatgatcg
     2341 cggcgaccag gcgggcctcc ccccggccgg ccaccctcag cacctggcgc ccgataagat
     2401 ttgc
//
LOCUS       DATDPQ010000003         4661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3401701, whole genome shotgun sequence.
ACCESSION   DATDPQ010000003 DATDPQ010000000
VERSION     DATDPQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3401701"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VI520_00030"
     CDS             <1..1033
                     /locus_tag="VI520_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HLF36298.1"
                     /translation="ELVRLVDRLKQVAPEIPQISTFELTTALAFMHFAEQDVNCGVIE
                     VGLGGRLDATNVITPAVCVITSLSYDHTHLLGQSLSEIAGEKAGIIKPGVPVVLAPQQ
                     TEAEQAIRLVAEQRGAPLHLVNRDWHYAAEERGLTGQSFRVWREAGGAAVQLSIPLLG
                     HHQVQNGCTAYVALQLAAEAGLPIGFEAIRVGLSSVGWAGRFQVLSVDPPVVLDAAHN
                     RDSALQLRIAMDDYFPGRPVRMVFGASSDKDVHGMLLELSPRVSRVILTQADHPRAEG
                     PEALAEQVRSFGHSVEVVVPVAEALRIAMQGMEEDEVVLVTGSIFVVGSALAAWEAIR
                     GQRVGAGQG"
     gene            1030..1917
                     /locus_tag="VI520_00035"
     CDS             1030..1917
                     /locus_tag="VI520_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36299.1"
                     /translation="MMRRWAAALVLGVAGTACRSSLVEFGIPADSLTGTPNSATAAAT
                     EVRPPTLAAGVGFSYAVVWVEEGLAVRQPAGITSSEVASLPADQRGIGVTGQSTLLGS
                     STWLEIFLPEGGTGWVNGYNLTEDVAPEPFCQDPRLVDLANRFILALESESGDQLAQL
                     ASPKRGLIIRHDWWNPEVVTELGSLSHLFQEAAGLDWGINRDSGLSITGSFGEVILPQ
                     LLDVFSAPPAFGCNRLATGSTAEEVAWPSEYTNLNFISFHRAAPSPNSLNWRTWALGI
                     EYVDGSPYLAVLVQYRGEI"
     gene            complement(1934..>4661)
                     /gene="mfd"
                     /locus_tag="VI520_00040"
     CDS             complement(1934..>4661)
                     /gene="mfd"
                     /locus_tag="VI520_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLF36300.1"
                     /translation="VGPAREGLPAYYQQSNDLPLPLAEAQPILEFFLPWMTPDPQTLL
                     DFLPTETLVIAEDLAGIQVAIGELEAQALELRTKQVEAKDLAREAPRPYLTVDEVMDG
                     LDRQGVVNLGTVSADSLEPEPFGEAFIPGPRFGGQLRPLLEFLAERRLAHETCVLVSR
                     QASRLADLWAAENAQRPLLERLPAQLAVGELYLMQAALSEGWSMRLADGARLHLLTDA
                     EVFGWSRPELRRRPRSRVPAPESAYADLAAGDPVVHVDYGVGIFEGLVARSLEGTERE
                     YLLVTYEAGDQLYVPISSADRITRYIGVDGAAPRLSRLGTAEWERVRSQASGAVEEVA
                     RELLELYARRMTAVGHAFSPDTHWQHELEASFPYVETEDQVSALQAVKADMERARPMD
                     RLICGDVGYGKTEVALRAAFKAVMDGKQVGLLVPTTVLAQQHYRTFQQRLAAFPVELE
                     MLSRFRSKAEIKQILELTQAGQIDIVIGTHRLLQKDVKFKDLGLLIIDEEQRFGVTHK
                     EHLKSLRSEVDVLTLTATPIPRTLYMALTGVRDISTIDTAPEDRLPVVTHTGPYDPGL
                     VRQAVLRELDRGGQVFFVHNRVQTISSVQLRLQRLLPEARIEMAHGQMPEQQLADVMS
                     AFTAGEVDVLLSTSIVESGLDIPNANTLVIDRSDQFGMAQLYQLRGRVGRGAVRAYAY
                     FFYHPGFKSTPEALERLEILGQHTQLGAGYSIALRDLEMRGAGDLLGVRQHGQIAAIG
                     FHFYTQLLAEAVRRLRGRQEGLEIPVEGLPPQVTIELPLASALTVEYVPDRRLRLQLY
                     RRLAGLRSLGAVDQLRAELSDRFGPPPVEAENLLYQLRVKILAAQTRVNSVSSENGRI
                     LLQLAPGVEPPDPLLLGEDIRASRRGLWLTGEPWQDRLLELLREIGELALPA"
BASE COUNT          842 a         1452 c         1531 g          836 t
ORIGIN      
        1 ggagctggtc cggttggtgg atcggctcaa gcaggttgct ccggagatcc cccaaatctc
       61 caccttcgag ctcacgacgg cgctggcctt catgcatttc gcggagcagg atgtgaattg
      121 cggggtgatc gaggtgggcc tgggtggccg gctggacgca accaatgtca tcacacctgc
      181 agtctgcgtg atcacttccc tgtcctacga ccacactcat ttgctggggc aaagcctgtc
      241 cgagattgcc ggcgagaagg cgggcattat caagccggga gtgccggtgg tgcttgcgcc
      301 gcagcagacc gaagcggagc aagcgatccg cttggtggcc gagcaacgcg gcgctccgct
      361 ccacctggtc aatcgggatt ggcactatgc tgcggaagag cgcgggttga ccgggcaaag
      421 ctttcgagtg tggagggagg caggaggcgc cgcggtccag ctcagcatcc cgctgttggg
      481 gcaccatcaa gtccagaatg gctgcacggc ctacgtcgcg ctccaactgg cagccgaggc
      541 cgggctgccc atcgggttcg aggcgatccg ggtggggctg agtagtgtgg gttgggcggg
      601 gaggtttcag gtgctgtcgg ttgacccgcc tgttgtgctc gacgccgccc ataatcgaga
      661 ttcggcgctc caactgcgga ttgccatgga tgactatttc cccggccggc cagtgcggat
      721 ggtcttcggt gcctcgagcg acaaggacgt ccacgggatg ctgctcgagc tctcgccgcg
      781 agtgtcgcga gtgatcctta cccaagccga tcatccacgg gcggaagggc cggaggcgct
      841 ggccgagcag gtccgcagct tcggccactc ggtggaggtc gtggtcccgg tggcggaagc
      901 actgcgcata gccatgcaag gcatggagga ggatgaggtg gtgctcgtca ccggcagcat
      961 ctttgtggtg ggatccgcgc tggctgcctg ggaggcaata cgggggcagc gggttggtgc
     1021 agggcaggga tgatgaggcg gtgggccgcg gcgctggttc ttggggtggc ggggaccgcc
     1081 tgccgctcgt cgctggtgga gtttggcata ccagcggact cgctgaccgg gacaccaaac
     1141 tcggcgaccg cggctgcaac cgaggtccgg ccgcccacgc tcgcggccgg ggtgggattc
     1201 agctacgcgg tggtgtgggt ggaggaaggc ctcgcggttc ggcaaccggc cgggatcacc
     1261 agttcagagg tcgcctcgct gcccgccgac cagcgcggaa tcggggtgac cggccagtcc
     1321 acgttgcttg ggagctcgac ctggctggag atcttcttgc cggaaggcgg gaccggttgg
     1381 gtgaatggct acaacctcac cgaagatgtc gctccggagc cattctgcca ggatccgcgc
     1441 ctggttgatc tggccaaccg gttcattctc gccctggaga gtgagagcgg cgatcaactc
     1501 gctcagctgg ccagcccaaa gcgcggactg attatccgac acgactggtg gaatcccgaa
     1561 gtagtcaccg aactcggcag tctctcgcac ctgttccagg aggcagcagg gcttgactgg
     1621 ggaatcaatc gagacagcgg gctgtcaatc accggtagct ttggtgaggt gatcttgccg
     1681 caattgctgg atgtcttctc cgccccgccg gctttcggct gcaaccggct cgccaccgga
     1741 agcacagccg aggaggtcgc ctggccctcg gaatacacca atctcaactt catttctttc
     1801 catcgagccg ccccttcgcc caacagtctc aactggcgga cctgggcgct ggggatcgag
     1861 tatgtagacg gatctccgta cctggcagta ttggttcagt atcggggcga gatctagtga
     1921 gcgctggctc ggatcaagcc ggaagcgcca gttctccgat ttcccgaagc agttccaaaa
     1981 gccgatcttg ccagggttcc cccgtgagcc acagtccccg ccggctggcc cggatatctt
     2041 ctccgagcag cagcggatcg ggcggctcga ccccaggggc gagctgaagc aggatccgtc
     2101 cgttctctga agagacggaa ttcacccggg tctgggcagc caggatcttg actcgcaact
     2161 gatataggag gttttctgct tcaaccggcg gcggaccgaa ccgatccgag agttcagccc
     2221 tcagttgatc caccgccccc agcgagcgga ggccggccaa cctccggtat agttggaggc
     2281 gcagccgccg atcgggcacg tactccacgg taagggcgct ggccagcggc aattcgatcg
     2341 tgacctgggg cggcagccct tccaccggga tctcgagccc ctcctgccgg ccccgcaatc
     2401 gccgaacggc ttccgccagc agttgggtat agaagtgaaa gccaatggcc gcgatctggc
     2461 cgtgctgtcg aaccccgagc aggtcgccgg ccccccgcat ctcgagatca cgcagcgcaa
     2521 tactgtagcc cgcgcccagc tgggtatgct ggccaaggat ctccagacgc tcgagcgcct
     2581 ccggagtcga cttgaatccc ggatggtaga agaagtaagc gtaggcccgg accgcgcccc
     2641 gcccaactcg tcccctcaat tgatagagct gagccattcc aaactgatcg gagcgatcga
     2701 taaccagggt atttgcattc gggatatcca atccagattc gacaatcgag gtgctgagca
     2761 gcacgtcgac ctcaccggcg gtgaaggcgg acatgacgtc ggccaattgc tgctccggca
     2821 tctggccgtg ggccatctcg attctcgcct caggcagcag tctctgcagc cggagctgaa
     2881 cactcgagat ggtctgaacc cggttatgga cgaagaacac ctggccgccc cgatcgagct
     2941 cccgaagtac ggcctgcctc accagacccg gatcatatgg cccagtgtgg gtaaccaccg
     3001 gcaaccggtc ttccggggcg gtatcgatag tcgaaatgtc cctcaccccc gtcagcgcca
     3061 tatagagggt gcgggggatc ggagtggcgg tcagggtcag cacgtcgacc tctgaccgca
     3121 ggctcttgag atgctccttg tgggtcactc cgaagcgctg ctcttcatcg atgatcaaca
     3181 gcccaagatc cttgaactta acgtccttct gcagcagccg atgggtcccg atcacgatat
     3241 cgatctgacc ggcctgagtc agttccaaga tttgcttgat ctcggccttg ctgcggaacc
     3301 tggacagcat ctcgagttcg acggggaaag ccgccaggcg ctgttgaaag gtccggtagt
     3361 gctgctgggc cagcacggtc gtcggaacga gcagaccgac ttgcttccca tccatgaccg
     3421 ccttgaaggc ggcccgcaag gcaacttcgg tcttgccata accaacgtcc ccgcagatca
     3481 accggtccat cgggcgggcg cgctccatgt ccgctttgac ggcctggagt gccgatacct
     3541 ggtcttcggt ctcgacatag ggaaaggagg cctccagctc atgctgccaa tgggtatcgg
     3601 gcgagaatgc gtgcccgacg gcggtcatcc gccgcgcata gagctcgagc aactcgcggg
     3661 cgacctcttc cacggccccg gacgcctgag atcggacccg ctcccattcg gcggtaccca
     3721 gtcgcgacaa tctgggtgca gctccgtcca ctccgatata acgcgtgatc cgatcggctg
     3781 agctgatggg aacgtaaagc tgatcgcccg cctcgtaggt gacgagcagg tattcccgtt
     3841 cggtcccctc caggctccgg gcgaccaggc cctcgaagat cccgaccccg tagtccacgt
     3901 gaacgaccgg gtcgccggcc gctaggtcgg cataggcgga ttctggggca ggtacccggc
     3961 tgcggggccg ccgccgcagt tccggccgcg accagccgaa gacctcggcg tctgtgagca
     4021 gatggagccg ggctccgtcg gcaagccgca tgctccaacc ttccgaaagg gccgcctgca
     4081 tgagatacag ctcacctacg gccagctgag ccggcagtcg ttccaacagc ggccgctggg
     4141 cattctcggc cgcccagaga tccgcaaggc gggatgcctg ccggctgacc aggacgcagg
     4201 tttcgtgtgc caggcggcgc tccgccagga attcgagcaa gggtcggagc tgccctccga
     4261 agcgcgggcc ggggatgaag gcctcgccaa acggctccgg ctccagggag tcggccgaga
     4321 ccgttccaag gttgacgacg ccctggcggt ccaagccatc catgacctcg tccacggtca
     4381 ggtacggtcg aggggcctcg cgagccaggt ccttggcttc gacctgcttt gtgcgcaatt
     4441 cgagcgcctg agcctcgagt tcaccgatgg ccacctggat tccagccaga tcttcggcga
     4501 tcaccagagt ctcggtgggg aggaaatcga gcaaagtctg cgggtcggga gtcatccagg
     4561 gaaggaagaa ttccaggatg ggttgcgcct cggcgagcgg gagaggcagg tcgttcgact
     4621 gctggtaata agcgggaagg ccctcccgag ccgggccgac c
//
LOCUS       DATDPQ010000004         4135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1048744, whole genome shotgun sequence.
ACCESSION   DATDPQ010000004 DATDPQ010000000
VERSION     DATDPQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048744"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="gatB"
                     /locus_tag="VI520_00045"
     CDS             complement(<1..802)
                     /gene="gatB"
                     /locus_tag="VI520_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLF36301.1"
                     /translation="MAEYEAVIGLETHIQVKTRSKIFCGCKADSWNDPPNVNICPVCT
                     GLPGVLPVLNHEVIGKAVRLASALQAQSIQSESYFARKNYFYPDLPKGYQISQYDEPL
                     ARAGQFELPLAGGERRQVQISKLHLEEDAGRTIYQAGRRLIDFNRCGVPLVEMVTGPD
                     LRSAEEVAQFLIRLRQLLRWLEISDADMERGHLRCDANVSIREPGSGQLNPKTEIKNV
                     NSISNIRQAVQAEIERQIREVEAGRTIESWTLDWDDERGVLSKMRSKES"
     gene            complement(795..2402)
                     /locus_tag="VI520_00050"
     CDS             complement(795..2402)
                     /locus_tag="VI520_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLF36302.1"
                     /translation="MSGQICDLPAHELVRQLKRGEFSAEEALESTLDRIERVEGRSGS
                     LENTSADPSDPTRVHAYITVSRERARQQSRAVDRALAAGEDPGPLAGVPVAIKDIFCV
                     AGTPSTAGSRILANFVAPYTATPAARLEAAGAVTIGKANLDEFTYGSSNESSAFTPTT
                     RNPWSTDRVPGGSSGGSAAAVAAGEATLSLGTDTSGSIRQPAAFCGVVGLKPTYGRVS
                     RYGLIAFASSLDCPGPLARNTTDAALMLGTIAGPDWRDATAANAPLADYLGALEAGVQ
                     GLRIGLSPDFLRLRTPDGETGEQREHALQPEIEQAVRRVADTLVGLGAEIVEDVRLPS
                     VQFGIPAYFVISRVEAASNLHRYDGVKYGHRSAAPVTDLSQLYRRSRGEGFGTQPKLR
                     ILMGMYVSGAQYEKRYYERALQVRTAIRRDFERAFDREGPFRLDALLAPTTPTTAFEL
                     GKVYGDSVLMQYADLLTVPANHAGIPALSIPAGLDSKGLPIGVQWMGPDFSEATLLRI
                     GRAYEQATADQPWRQLRPAILRELPNG"
     gene            complement(2399..2734)
                     /locus_tag="VI520_00055"
     CDS             complement(2399..2734)
                     /locus_tag="VI520_00055"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLF36303.1"
                     /translation="MAEALNREPIDDRVFQHLVQLAALELRADEAEYLRRELNAQLAA
                     IRNLEAIQIPADLPITSHGVPYSPAIRPGPRPDQIRPSELADAILSQAPEVTDRYIVV
                     PDLPRTDLE"
     gene            2823..4007
                     /locus_tag="VI520_00060"
     CDS             2823..4007
                     /locus_tag="VI520_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF36304.1"
                     /translation="MVTEIAHPGPNLQLTAEHEQIRQLAREFAQKEIAPIAAEFDERG
                     EFPLATVRKMGQMGFMGIEVPEEYGGAGLDSLAYVLALIEISKADASHGTIMSVNNSL
                     FCYALQRFGSAAQKEEFLRPVASGEAIGAYSLTEPMSGSDAGTMRSRAVRDGDVYVIN
                     GRKSWVTSGPVAKYILLFTMTEPEQKHRGVTAFLIETDRPGFSVGKIEPKLGIRASAT
                     CEVLFENYRCPVEDRLGEAGEGFKIAMHALDAGRIGIASQALGIAEAAYEASLTFARQ
                     REAFGQPIGEFQGIGFKLADMKTRIEASRLLIYQAALAKQAAKLSGEAFTLEASMAKL
                     FASETAGFCTNHAVQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRQELG
                     LR"
BASE COUNT          738 a         1320 c         1303 g          774 t
ORIGIN      
        1 cgctctcctt ggaacgcatc ttgctgagca cccctcgctc atcgtcccag tccagggtcc
       61 aggactcgat cgttcgcccc gcctcgacct cccggatctg ccgctcgatt tcggcctgga
      121 cggcctgccg gatattgctg attgagttca cgttcttgat ttcggttttt ggattgagct
      181 gcccgctgcc tggctcgcgg atcgaaacgt tggcgtcgca gcgcagatgg ccccgttcca
      241 tgtctgcatc ggaaatctcc agccagcgca gcagctgacg cagccggatc aggaattggg
      301 ccacctcttc ggcggagcgc agatccggcc cggtcaccat ttccaccagt ggaacgccgc
      361 agcgattgaa gtcgatcagc cgccgcccag cttggtagat cgtgcggccc gcgtcttcct
      421 ccaggtgcag cttgctgatc tgcacctgtc gccgctcgcc ccccgccagg ggcagctcga
      481 actgtcccgc tcgtgccagc ggctcgtcat actgactgat ctgatatccc ttgggaagat
      541 ccggatagaa gtagttcttg cgggcgaagt acgattccga ctggatcgat tgcgcctgca
      601 gggcggaggc cagccgtacc gccttgccga tcacttcatg gttcaagacc ggcagcaccc
      661 caggcagtcc ggtgcacacc gggcagatat tgacgttggg cgggtcgttc caggagtccg
      721 ccttgcatcc gcagaagatc ttagaacggg tcttcacttg gatgtgggtc tcgagcccga
      781 tgacggcctc gtactcagcc attcggcagc tcccgaagaa tcgcgggtct gagctgtctc
      841 cagggctggt cggcagtcgc ctgctcgtag gctctgccaa tcctcagcag agtggcctcc
      901 gagaagtcgg gccccatcca ctgcacccca atcggcagcc ccttggagtc gaggccggct
      961 gggatcgaga gcgccgggat accggcatga ttggcgggga ccgtcaacag atcggcgtac
     1021 tgcatcagca ctgaatctcc atacactttc cccagctcaa aggcggtcgt gggggtcgtt
     1081 ggggcgagca gcgcatcgag ccggaagggc ccttcccgat caaatgcccg ctcgaaatcc
     1141 cggcggatcg cagtgcggac ctgcagggcc cgttcgtagt atcgcttctc gtactgggcg
     1201 cctgaaacgt acatccccat caggatccgc agtttaggct gagtgccgaa accttccccg
     1261 cgactgcgcc ggtacagctg actcaaatcg gtgaccggtg cggcggagcg atggccgtac
     1321 ttgactccgt cgtagcggtg caggttcgag gcggcttcca ctcgcgagat gacgaagtag
     1381 gccgggatgc cgaattgcac gctcggcagg cggacgtctt ccacaatctc ggcgcccagc
     1441 ccgaccaagg tgtcggccac ccgacgaacc gcctgctcaa tctccggctg cagggcgtgc
     1501 tctcgctgtt caccggtctc cccatccggg gtgcgcagac gcaggaaatc gggggagagg
     1561 ccgatgcgca gtccctgcac tccggcctcg agcgcaccga ggtaatccgc aagcggcgcg
     1621 ttggcagcag ttgcatcccg ccagtccggc ccggcgatag tccccagcat cagggccgcg
     1681 tcggtcgtat tccgagcgag tgggcccggg caatccagtg atgaggcgaa tgcgatcaac
     1741 ccgtacctgg aaacccttcc ataggttggc ttgagcccga cgactccaca gaaggccgct
     1801 ggctggcgga ttgatccgga ggtgtccgtc cctaacgaga gggtggcctc gccggcggcc
     1861 accgccgcgg cgctgccgcc ggaagatccc cccggcactc ggtccgttga ccagggattg
     1921 cgagtcgtcg gagtgaaggc ggacgactca ttcgacgacc cgtaggtaaa ctcatccagg
     1981 ttcgccttcc cgatggtcac tgccccggct gcctccagcc tggccgccgg cgtggcggtg
     2041 tagggggcaa cgaagttcgc caggatccga gatccggcgg tcgatggagt gccggcgaca
     2101 caaaaaatgt ccttgatcgc caccgggact ccggccagcg gcccaggatc ctctccagcg
     2161 gccagcgccc gatcgacggc gcgggactgc tgccgcgccc gttcgcggct aaccgtgatg
     2221 taggcatgca cccgggttgg gtctgacgga tcggcgctgg tgttttcgag gctgccagaa
     2281 cgcccttcca cccgctcgat ccggtctagg gtcgactcga gcgcctcttc cgcgctgaac
     2341 tcgccacgct tcagctggcg gaccagctcg tgggcgggca gatcgcagat ctgcccactc
     2401 attccaagtc ggtgcgggga agatcgggga cgacgatgta gcgatccgtc acttcggggg
     2461 cttggcttaa gatcgcatcg gccagctccg agggtcggat ttgatccggg cgcgggccgg
     2521 gtcggatggc tgggctgtag ggcacgccgt gggaagtgat cggcaagtcc gccgggatct
     2581 ggatggcttc cagatttcga atcgcagcga gctgagcgtt caattccctg cggaggtact
     2641 cggcttcgtc cgctcgcagc tcgagcgcag ccagctgaac caagtgttgg aacacccggt
     2701 catcaatcgg ttcacgattc aaagcttcgg ccatgctgag cctctccggg tggccattgt
     2761 acctgagggc ttcgtctgac cctgcgtgct ataatcgccc cgcctcgccc cacccacccg
     2821 caatggtcac cgaaatcgct catccgggcc caaacctgca actcacagct gagcacgagc
     2881 agatccgcca gctcgcccgc gagtttgctc agaaagagat cgcaccgatt gcggccgaat
     2941 tcgatgagcg gggggaattt ccgctggcga ccgtccgcaa gatgggccag atgggcttca
     3001 tggggatcga ggtgcccgaa gagtacggcg gggccggctt ggacagcctg gcctacgtgc
     3061 tggccctgat tgagatcagt aaggccgatg cctcccacgg gaccatcatg tcggtgaaca
     3121 attcgctctt ctgttacgcg ctgcagcggt tcgggtcggc ggctcagaag gaagagtttc
     3181 tccgcccggt agcctcgggc gaggcgatcg gtgcctattc gctgaccgaa cccatgtcgg
     3241 gatcagacgc cgggacgatg cgttcgcggg ccgtccgcga cggggacgtg tatgtcatca
     3301 atggccgcaa gtcttgggtg accagcgggc cggtggcaaa atatatcctg ctcttcacga
     3361 tgaccgagcc cgagcaaaaa catcggggag tcacggcctt tctaattgag accgaccggc
     3421 ccggcttcag cgtcggaaaa attgagccga agctcggcat tcgggcgagc gccacctgcg
     3481 aggttctctt cgaaaactat cgctgtccgg tggaggaccg gctgggcgaa gcgggagaag
     3541 gtttcaagat cgccatgcac gcgctcgacg cgggccggat cggaatcgcc tcgcaagccc
     3601 tcggcatcgc cgaagcggcc tacgaggcca gcctgacgtt cgcccgccag cgggaggcct
     3661 tcggccagcc gatcggtgag tttcagggga tcggcttcaa gctggccgac atgaaaaccc
     3721 ggatcgaagc cagccggctg ctgatctatc aggccgcctt ggccaaacag gctgccaagc
     3781 tcagtggcga ggccttcact ctggaagcct ccatggccaa actgttcgca tccgagaccg
     3841 cgggtttttg tactaaccac gcggtgcaga tccacggggg gatgggttac tccaaagagc
     3901 tgccggtcga gcgttacttc cgggatgcca agatcaccga gatctacgag ggcacgagcg
     3961 agattcagcg gctggtcatc tctcgccagg agctgggact tcgctagcgc ctgacctcgc
     4021 ccagctcacc gtttgctcca tttttcacaa atgatacctt ttcaccgcta gcacctctag
     4081 tggattggcg ggaacgctgg tttgacccat actgaccagc ccgcatcgag ggtat
//
LOCUS       DATDPQ010000005         3355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_786443, whole genome shotgun sequence.
ACCESSION   DATDPQ010000005 DATDPQ010000000
VERSION     DATDPQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786443"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VI520_00065"
     CDS             <1..386
                     /locus_tag="VI520_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36305.1"
                     /translation="PTPVTGEMMGTDMNVVLPPGDAEAGRALAEGTLGCAACHILSPV
                     GPPWAATDEQPGIAVRAGVRIGEDGYLGEATTAEEYIIESVVRPNAYVVAGYPDNPNP
                     MPPNFGERITLQNMADLLAHMLTFR"
     gene            551..1324
                     /locus_tag="VI520_00070"
     CDS             551..1324
                     /locus_tag="VI520_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF36306.1"
                     /translation="MRTLRAIGISLAAALLGWTAAVAQTEGDPERGGQLYVENCAVCH
                     GVDGQGRIGARLEAFSGIDPSAVIAQTVAQGIEGTIMPAWGAANGGPLSDQDIADIVA
                     YVVSAFNGTEPIEPLPEYVPPEIARLPDVEGDPSLGAVVYQANCAVCHGEQGRGRIGK
                     PLATSWPGAQPELFIRQVVSDGIVGTKMPTWAQSNGGPLSDEEIANVAAYLLTLPSAG
                     SSPAPTAQEGPIGVSTALLVLAGLVGLAAVIGIAYYRRA"
     gene            1433..2236
                     /locus_tag="VI520_00075"
     CDS             1433..2236
                     /locus_tag="VI520_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36307.1"
                     /translation="MWPEYDRAATLVIYRFQLGENTPLPAVVELPIPSSVGEPNAVAW
                     QDADQALFDAAHTIDAAGDWATVRIEMEQSRTGQLEYYADLQIVDDLRSFRFDWPGGV
                     QIEASAYEVQQPVTATELTIVPEADRQSSGPFGLTYHLAELGPQSVGVSWAIELSYRK
                     ADTILTVETLQPLGEPAAAASAARDSSALLPWVLGGLGLAAVGGGLAYYLRSRPAPAA
                     PRRRRGGTAEPTLEASAIFCHTCGTKAHVSDRFCRNCGTRLRTSPDHPS"
     gene            2413..3006
                     /locus_tag="VI520_00080"
     CDS             2413..3006
                     /locus_tag="VI520_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HLF36308.1"
                     /translation="MATQALSTKAYLEKRRQETRRKRGLAAGLVLVGALLVGAAFWLS
                     SAGRASAVTSFDYRPEDVARAEPFQAIHDMEQGPPIPFLPNDGPQPEIAVNERFYDFG
                     SIGPTEVVTREFVVANLGDAPLTISRAYTTCGCTTADFTSSVIPPGEVVLVTLRFDAG
                     FHDTAGQTVRRGIIIENNDPDRSSAEIWVQAAVRTNS"
     gene            3003..>3355
                     /locus_tag="VI520_00085"
     CDS             3003..>3355
                     /locus_tag="VI520_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLF36309.1"
                     /translation="MSEPAGGPSDPNLSRRAWVRPVAYGSLLALLGLVGWGLVRAQAG
                     PRDQGPAPDFSLATFSGEQVSLSALRGQVVVINFWASWCPPCREEAAYLESTWRAYRD
                     QGVVFIGVDYADTDR"
BASE COUNT          591 a         1061 c         1116 g          587 t
ORIGIN      
        1 agccgacccc agttaccgga gagatgatgg gcaccgacat gaatgtcgta cttcccccgg
       61 gcgatgcgga ggccggtcgg gcgctggcgg agggaaccct cggctgcgcg gcctgccaca
      121 tcctgtcgcc ggtcggcccg ccctgggccg caaccgatga acaacccggg atagccgtgc
      181 gggccggggt ccgcatcggg gaggacggct acctcgggga ggcaaccacc gccgaagagt
      241 acatcatcga gtcggtggtc cgacccaatg cgtatgtagt ggcaggctac ccggacaacc
      301 ccaaccccat gccccccaat tttggggagc ggattactct gcagaacatg gccgacctgc
      361 tggcccatat gttgaccttc cgctgagccg aagcgcggac tgagcagaat ctatgacgcc
      421 gcggcctcct ttgaggcggc ggcgtctctc acaagaaagc agacggagag cggagcatcg
      481 tcactagttg tcgatcggaa cgtcccgcta caatcgatca ggcaggccga gctgctttgg
      541 gaggccaagc atgcgaacac tgagagcgat cgggatctcg ctggcggctg cgttgttggg
      601 atggaccgcg gcagttgcgc agaccgaagg cgatcctgag cggggtggtc agctctacgt
      661 cgagaattgc gccgtgtgtc atggggtaga cggtcagggc cgcatcgggg cgcggctgga
      721 ggcgttctcc ggcatcgacc cgagcgcggt catcgcccag actgtcgccc aggggattga
      781 gggaaccatc atgccggcct ggggagcggc gaacggcggg ccgctgtcgg atcaggacat
      841 tgccgacatc gtcgcctatg tcgtgagcgc cttcaatggc acggagccga tcgagccgct
      901 gccggagtac gtgccgcctg agatcgcccg gctcccggat gtggagggcg atccgtcgct
      961 cggggcggtc gtgtatcaag ccaactgtgc tgtctgtcac ggtgagcagg gtcgggggcg
     1021 catcggcaag ccgcttgcaa cctcctggcc gggcgcccag cccgagctct tcatcagaca
     1081 ggtggttagt gacgggattg tgggcaccaa gatgccgacc tgggcgcaat ccaacggtgg
     1141 accgctgagc gacgaagaga ttgccaacgt agctgcctat ctgctgaccc tcccttcggc
     1201 cggctcctcg cccgcgccaa ccgctcaaga gggtccgatc ggggtaagca ctgcgctgct
     1261 ggtcttggcc gggctggtcg ggttagccgc cgtgattggt atcgcctact accggagggc
     1321 ctgaacggca cacccatgga tcgcggcggc cgggctgttt tgcctcacgg tctggccgtc
     1381 acaagttcgg gctcaagcga cgactgccga gctgcagcgc cttgaaatcg ccttgtggcc
     1441 cgaatacgac cgggccgcga ccctggtgat ctatcgcttt cagctgggag agaatactcc
     1501 tctcccggca gtggtcgagc tgccgattcc gtcctcggtt ggggagccca acgcggtggc
     1561 atggcaggac gccgatcagg cgcttttcga cgctgcccac accatcgatg ccgccggcga
     1621 ttgggcaacc gtccggatcg agatggagca gagccgcacc ggtcagctcg agtactacgc
     1681 cgacctacag attgtcgatg acctgcggag ctttcggttc gattggccag ggggagtcca
     1741 gatcgaggcc tcagcctatg aagttcagca accggtcacg gccacggaac tgacgatcgt
     1801 gcccgaggcg gatcgacaaa gctccggccc ctttggactc acttaccacc tggctgagct
     1861 cgggccccaa tctgtgggtg tcagctgggc gatcgaactc agctaccgca aggccgatac
     1921 gattctaacg gtggagacgc tccagccgct cggagagccg gccgcggcgg cgagcgccgc
     1981 ccgcgactca tctgcgctcc tgccctgggt gctgggcggg ctagggctcg cggcggttgg
     2041 cggagggctc gcatactatc tccgctcgcg gcccgctccg gccgcgcctc gacgccgtag
     2101 aggcggcacc gccgagccta ccctcgaagc ttcggcgatc ttctgccaca cgtgcggtac
     2161 caaagcacac gtcagcgatc ggttttgccg caactgcggc actcggctgc gcacctcccc
     2221 tgatcatccg tcgtaaatag cggcgagcca gcctcgctcg gatcactaac cacgcaacag
     2281 ttctaagtct gcccccgtac tcaaaacgaa tgtcatagga cgggccaaat ggcgcttatc
     2341 ggcttggcca gcctttgcta ccttgatgac gattatccga gcctggatga attcggcaga
     2401 ggaaggtgtc agatggcgac ccaagcattg agcacgaagg cgtacctgga gaagcgcagg
     2461 caggagacgc ggcgcaagcg aggtctcgcc gctgggctgg ttctggtcgg cgccctgctg
     2521 gtcggcgcgg cattctggct atcctctgcc ggacgcgctt cggcagtcac cagcttcgat
     2581 tatcgtccgg aggacgtcgc ccgggcggag ccgtttcagg cgatacacga tatggagcag
     2641 gggccaccga tccctttcct gcctaatgac ggtccgcagc cggaaatcgc ggtcaacgag
     2701 cgcttctatg acttcggcag cattgggcct acggaggttg tgactcgcga attcgtcgtg
     2761 gccaacctgg gagacgcgcc gcttaccatc agccgggcct acacaacctg cggctgcacg
     2821 acggctgact tcacttcgtc cgtaattcca cctggcgagg tggtgctggt caccctgagg
     2881 ttcgatgccg gattccatga cacggccggg cagaccgtgc gccggggaat catcatcgag
     2941 aacaacgatc cagatcggtc ctccgctgag atctgggttc aggctgccgt ccgaaccaac
     3001 tcatgagcga gcccgctgga gggccgtcgg acccgaatct aagccgacgg gcctgggtca
     3061 ggccagtggc gtacggcagc ctgttggcgc tgctcggcct tgtcggctgg gggctcgtcc
     3121 gggcgcaggc cggcccaagg gaccagggtc ccgcaccgga cttcagcttg gccaccttca
     3181 gcggggagca ggtcagcctc agcgcattgc gaggccaggt ggtggtgatc aacttctggg
     3241 cctcctggtg cccgccctgc cgggaggagg cggcctacct ggaaagcacc tggcgagcct
     3301 accgggatca aggggtggta ttcatcggag tcgattacgc cgatacggac cgcga
//
LOCUS       DATDPQ010000006         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1571421, whole genome shotgun sequence.
ACCESSION   DATDPQ010000006 DATDPQ010000000
VERSION     DATDPQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571421"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VI520_00090"
     CDS             complement(<1..701)
                     /locus_tag="VI520_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HLF36310.1"
                     /translation="MKVRAITGFLDPGWPLVPERIAALAECLRAIRQALGDAGYETQT
                     LRLATPPPSRIDKPVPPSDRPELARQLAAESLSHGIEYTNIGPALPDELDGFRVVPEV
                     LAAAESVFCSGLYASVPSGLSLPAARACAEAIQAASTVSPDGFANLRFAGLANVGPGS
                     PFFPAAYHYGGPAAVGLATESADLAVESFQNAISLAAARRQLVSLIETHAAALTRAAE
                     PVAAEHDVRFLGIDFS"
     gene            complement(747..1100)
                     /gene="rplT"
                     /locus_tag="VI520_00095"
     CDS             complement(747..1100)
                     /gene="rplT"
                     /locus_tag="VI520_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLF36311.1"
                     /translation="MTRVKGGTVTRKRHKKVLKLARGYYGTRGRLFRRSNEAKLKSLS
                     YAYRDRRNRKRDLRSLWIARINAGARQNGTSYSRLIRAMGQSNIQINRKMLAELAVRD
                     PKAFSSVVKAASPGS"
     gene            complement(1102..1377)
                     /locus_tag="VI520_00100"
     CDS             complement(1102..1377)
                     /locus_tag="VI520_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HLF36312.1"
                     /translation="MPRKKKLKGKVKLKTHKATSKRFRLTSTGKLVRTKGGKSHLRRH
                     KSKRTKRLFEEMLPVEGSGIRKRVHRLAPYLSKRKAKPIARSGGASK"
     gene            complement(1467..1991)
                     /gene="infC"
                     /locus_tag="VI520_00105"
     CDS             complement(1467..1991)
                     /gene="infC"
                     /locus_tag="VI520_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HLF36313.1"
                     /translation="MQDISASEFRINGAIRVPEVRLIGADGVNVGVVKTAEAMAAARL
                     AGLDLVEVAPNAEPPVVRVLDFGKFLYEKAKKDREARKSQTRIEIKEIRLRPKTSEHH
                     RSFKVRNARRWLSEGKKVLARIRFRGREITYPEIALEDLKRIAEDLADVGVIEQSPRM
                     EGRTMFMVLGPNKK"
     gene            complement(2107..2487)
                     /locus_tag="VI520_00110"
     CDS             complement(2107..2487)
                     /locus_tag="VI520_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36314.1"
                     /translation="MRAAVVILLLVATSAIFQSRSWGWQTTPAVVAALSLLTLSGALA
                     GTSGLIASWRAARRKTRIRPFLLNGAAALAALAIWLNYVLTELAESEGSPTQRGLLAL
                     ALAWMISPFAGLALTSLGDRLARR"
     gene            complement(2484..>3101)
                     /locus_tag="VI520_00115"
     CDS             complement(2484..>3101)
                     /locus_tag="VI520_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36315.1"
                     /translation="DTQLEILAILAQARGAQEFRAMLPQAVAHARAKLRELQAGRVPA
                     KELVVTHRLSRQLEEYRVRTPAARAAGQLARAGVELSPGEMMHFIYVSGPEKVRAWEA
                     MEADPIYDSQAYTELLLRAVESLLAPVGVDRATLETWLLADAGYWGPPGILPPAGADI
                     GAPLMERPRLPAAGLDFRSRPVPRRPLHIPLAADYKTPIAQEKAA"
BASE COUNT          513 a          990 c         1027 g          571 t
ORIGIN      
        1 gagaagtcaa tccccaggaa ccggacgtca tgttctgcgg cgacgggttc ggcggctcga
       61 gtgagggccg cagcatgggt ctcgatcaag ctgaccagct ggcggcgagc cgcggcgagc
      121 gagatggcat tctgaaaact ctccaccgcc agatcggcag actcggttgc cagcccgacc
      181 gccgcgggtc caccgtaatg ataggcggct gggaagaacg gcgatccggg cccgacgttg
      241 gcgagccccg caaatcgtaa attggcaaaa ccatctggag acactgtgga ggcggcctga
      301 atcgcctcgg cgcaggcccg ggcagccggg agcgagagac cggagggcac actggcgtac
      361 agcccggaac aaaacacgct ctccgccgca gccagaactt ccggcacgac tcggaatcca
      421 tccagctcgt cggggagcgc cggcccgatg ttggtgtact cgatgccgtg gctcaggctc
      481 tcggccgcca actgccgggc taattctggg cgatcggagg gcgggactgg cttgtcgatc
      541 cgggagggcg gcggagtagc cagccgcagg gtctgggtct cgtagccggc gtcgccgagc
      601 gcttgccgga tcgccctcag gcactcggcc agggccgcga ttctctccgg caccagcggc
      661 caaccagggt cgaggaagcc ggtgatggcg cgaaccttca tgtgagcttc agtgggttag
      721 ggggagaatg accgcggggt ctgtcgtcag gagccggggc tagcggcctt gacgacggaa
      781 gagaatgcct tggggtcgcg cactgcgagt tcagccagca tcttccggtt gatctggata
      841 ttggactgtc ccatcgcgcg gatcagccgg ctgtagctgg tgccgttctg ccgggcgccg
      901 gcattgattc gggcgatcca aagcgatcgc aggtcgcgtt tgcggtttcg ccggtctcga
      961 taggcgtagc tgaggctctt gagtttggct tcgttcgagc ggcgaaagag ccggccccgg
     1021 gtcccgtaat acccgcgggc aagcttgagg accttcttat ggcgcttgcg agtgaccgtt
     1081 ccgcccttaa ctcgggtcat gttacttcga cgctcctccg ctgcgggcga tgggtttggc
     1141 tttgcgtttc gagaggtagg gagccaggcg gtgcactcgc ttgcgaatcc ctgagccctc
     1201 gaccggcagc atctcctcga acagtcgttt ggtgcgcttc gacttatgtc ggcgcaggtg
     1261 gctcttgcca cccttcgttc gaaccagctt cccggttgag gttaaccgga agcgcttgga
     1321 ggtcgccttg tgggtcttaa gcttaacttt gcccttcaac ttctttttcc gcggcacctg
     1381 gatgctcctg cctgccgttc acgaaagccg ccgggacggt gagccgcccg gacggcgagg
     1441 ctgcatgctg aaccgaaagc tacggactac ttcttgtttg gaccgagcac catgaacatg
     1501 gtccgccctt ccatccgggg cgattgctcg atcactccaa cgtctgcgag atcttccgcg
     1561 atccgcttca aatcttccag cgcaatctcc gggtaggtga tctcacgacc ccggaagcga
     1621 atccgggcca acaccttctt gccttctgac aaccagcgcc tggcgttgcg gaccttgaac
     1681 gagcgatggt gctcggaggt cttggggcgc agtcggatct ctttgatttc gatcctggtc
     1741 tgcgacttgc gggcctcccg gtctttcttc gccttctcgt agagaaactt tccgaaatcc
     1801 agcacccgga cgaccggggg ttctgcgttt ggcgcgactt cgacgagatc caacccggct
     1861 aatcgggccg cggccatcgc ctcggcagtc tttactacac ctacgttcac gccgtccgca
     1921 ccgattaagc ggacttcagg taccctgatc gccccgttta tcctgaattc cgatgcgctg
     1981 atgtcctgca cctcccaaat aggaaatcga agccgctcct cggcggcttg cgagcatcat
     2041 accatgcgtc cggtttggga gtcaaaccgt gggtactcat cgtgagtttg cgggtagcct
     2101 cgggactcag cgtcgggcga gtcgatctcc tagggaggtg agggccaacc cggcgaaggg
     2161 cgagatcatc cacgccagcg ccagggccag cagcccgcgt tgggtcggcg acccctccga
     2221 ctcggcgagc tcggtgagca cgtagttcag ccagatcgcc agggccgcta gcgcggctgc
     2281 tccattgagc aggaacggcc ggatccgggt cttccggcgg gctgctcgcc aggaggcgat
     2341 gagcccggag gtcccggcca gcgcgccgga gagggtaagc agcgagagcg cggccaccac
     2401 cgcgggggtt gtctgccagc cccaggaacg gctctggaag atggcgctgg tggcgaccag
     2461 cagcaggatc accacggcgg ccctcatgct gccttttcct gagcgatcgg agtcttgtag
     2521 tctgcggcca gcgggatatg caacggccgg cggggaactg gcctggaccg gaagtccaat
     2581 ccggcagcgg gcagccgcgg ccgctccatc agaggcgcgc cgatgtccgc cccggccggg
     2641 ggaaggatgc cgggcggtcc ccaatacccg gcgtcggcca gcagccaggt ctcgagcgta
     2701 gctcggtcga ccccgaccgg cgccagcagg ctttccactg cccgcagcag cagttcggta
     2761 taggcctggc tgtcgtagat cgggtcggcc tccatcgctt cccaggcccg gactttttct
     2821 ggccccgaaa cgtagatgaa gtgcatcatt tcccccgggc tgagctccac gccggcccga
     2881 gccagctgcc cggcggcccg agcggcgggg gtgcggactc ggtactcctc gagctggcgc
     2941 gacagccgat gggtgacgac caactctttg gccggcaccc ggccggcctg cagctcgcgg
     3001 agcttcgccc gagcatgggc cacggcctgc ggcagcatcg cccgaaactc ctgcgcgccg
     3061 cgagcctggg ccaggatggc caggatctcc agttgggtgt c
//
LOCUS       DATDPQ010000007         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_264200, whole genome shotgun sequence.
ACCESSION   DATDPQ010000007 DATDPQ010000000
VERSION     DATDPQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264200"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VI520_00120"
     CDS             <1..510
                     /locus_tag="VI520_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HLF36316.1"
                     /translation="SCVRYGNVVGSRGSVVPVFLRQRANGRVTVTDERMTRFWLSIDQ
                     GVRFTIRCIELMQGGEVFVPKIPSMRIVDVAAAVAPGCEIDFIGIRPGEKLHENLLSE
                     DEGRNAVELDEMYVVQPGGALWFGHTWEGKGKSLPEGFRYGSETNEQWMSVEAIRQMA
                     ESHPGGQEG"
     gene            513..1436
                     /locus_tag="VI520_00125"
     CDS             513..1436
                     /locus_tag="VI520_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF36317.1"
                     /translation="MAKILISGASGLLGSNLAVVAATGHRAQGIYHQHPLQIPGVGMV
                     AGDLADREQASRVIEAFAPDWVVHCAAASEVDRCELDPAWAWRLNLEMAKNVAEAAHE
                     AGARLAHVSTDAVFDGERGDYRESDEPRPIMTYGVSKLAGEQAVALAHPEALILRINL
                     FGWGPGPKRSLAEWFLDRLETGDSCPGFTDVYFSPLVAQQLARAILDLLATGASGLFH
                     LPGRTCLSKYEFGVLLAQQFELDPARIRPMSVGEAKLTAARPKRLCLNGEKVESVLGR
                     RLPELASGLRELRESRGMLPSRRQAKAGTAA"
     gene            1433..>2182
                     /locus_tag="VI520_00130"
     CDS             1433..>2182
                     /locus_tag="VI520_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HLF36318.1"
                     /translation="MSEIEIAGRKIGGSHSTYFIADIAANHDGDLERAKQLIRLAAEA
                     GAEAAKFQNFRAPQIVSDFGFRSLSAQASHQAKWKKSVFEVYAEASIPFDWSAELAEE
                     CAEAGIHYFSSPYDFEAVDRLEAYFPAIKIGSGDITWIEIVQRIASKRKPVLLATGAS
                     TLGDVQRAVEAILAINSDLVLLQCNTNYTASLENFDHIHLNVLKSYGLMWPELVLGLS
                     DHTPGHATVLGAVALGARVVEKHFTDDTRREG"
BASE COUNT          391 a          625 c          777 g          389 t
ORIGIN      
        1 agctgtgtcc ggtacggaaa tgtggtcggc agccgcggca gcgtggtgcc ggtcttcctg
       61 cgtcagcgcg caaacggccg agtgacggtg accgacgagc gcatgactcg gttttggctg
      121 tcgatcgacc agggtgtccg attcacaatt cgatgcatcg agctgatgca ggggggtgaa
      181 gtgttcgtac caaagatccc cagcatgcga atcgtcgacg tagccgccgc ggtggctccc
      241 ggttgcgaga tcgacttcat cggaatccgg ccgggtgaga aactgcatga gaacctgctg
      301 tcggaagacg aaggtcggaa cgcggtcgag ctggacgaga tgtacgttgt ccagcctggc
      361 ggggccttgt ggttcgggca tacctgggaa ggcaagggaa agtctcttcc ggagggattt
      421 cgctacggca gtgagaccaa tgagcagtgg atgagcgtgg aggcgatccg ccagatggca
      481 gagtctcatc caggcgggca ggagggctga gggtggccaa gatcctgatc agcggagcca
      541 gcggtctgct gggcagcaat ctggccgtgg tggcggcgac cgggcaccga gcccagggca
      601 tctaccatca gcatccgctt cagattccgg gcgtcgggat ggtggcgggc gacctcgccg
      661 atcgcgagca agccagccgg gttatagaag cgttcgcacc ggactgggtc gtgcactgtg
      721 cggccgccag cgaggtcgat cgctgcgagt tggatccggc ctgggcctgg cggctgaacc
      781 tggagatggc gaaaaacgtg gccgaggcgg cccacgaagc gggggcgcgc ctggcccatg
      841 tgtcaaccga tgcggtgttt gacggagagc ggggagatta ccgggaaagc gacgagccgc
      901 ggccgatcat gacctacggg gtgtcgaagc tggctggtga gcaagccgtg gccctggccc
      961 atccggaagc cctcattctg aggatcaacc tctttggttg ggggccggga cccaagcgca
     1021 gcctggccga gtggtttctg gatcgactgg agaccggcga ttcgtgcccg ggattcaccg
     1081 acgtctattt ctccccgttg gtggcccaac aacttgcccg ggcgattctt gatctgctgg
     1141 caacgggtgc cagcggcctc ttccacctgc cggggcgcac ttgtttgagc aagtacgagt
     1201 tcggcgtgct gttagcgcag cagtttgagc tggatccggc ccggatccgg ccgatgagcg
     1261 tcggggaggc gaagctgacg gccgcccggc ccaagcggct gtgcctgaac ggcgagaaag
     1321 tcgagagcgt gttggggcga cggcttccag agctggcgag cgggctgcgt gaattgcgcg
     1381 aatcacgcgg aatgctcccg agccggcgcc aggcgaaggc ggggaccgcg gcgtgagcga
     1441 gatcgagatc gccggccgga agatcggtgg cagccattcc acctatttca tcgccgacat
     1501 tgccgccaat cacgatggcg acctggaacg ggcgaaacag ctgatccgct tggccgcgga
     1561 ggccggggca gaggcggcca agtttcagaa cttccgtgcc ccgcagatcg tcagcgactt
     1621 cggtttccgc agcctgtctg cccaggcctc ccatcaggcc aagtggaaga aatcggtctt
     1681 cgaggtctac gccgaggcct caatcccttt cgattggtcg gcggagctgg ccgaagagtg
     1741 tgccgaggcc ggcatccact acttttcgtc tccctacgat ttcgaggcgg tggatcggct
     1801 ggaggcctat ttcccggcga tcaagatcgg ctcgggcgac attacctgga tcgagatcgt
     1861 gcagcggatc gcctccaagc ggaagccggt gctgctggcg accggcgcct cgaccctcgg
     1921 cgacgtgcag cgggcggtgg aggccatcct ggcgatcaac tcggacctgg tgctgctgca
     1981 gtgcaatacg aactacaccg ccagcctgga gaacttcgat cacattcact tgaatgtgct
     2041 taagtcctac ggattgatgt ggccggagct ggtgctgggg ttgtcggacc acactccggg
     2101 gcatgccacg gtcctcggtg cagtcgcgct gggcgcccgg gtggtggaaa agcatttcac
     2161 cgacgacacc cggcgggagg gg
//
LOCUS       DATDPQ010000008         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_264599, whole genome shotgun sequence.
ACCESSION   DATDPQ010000008 DATDPQ010000000
VERSION     DATDPQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264599"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1164
                     /locus_tag="VI520_00135"
     CDS             127..1164
                     /locus_tag="VI520_00135"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLF36319.1"
                     /translation="MPDGIEASAPLSRREFVRLSATGLLALGIPAQRTRAAAEAEPGL
                     LGRVVDPTLDIFSGPSFASEKRKTLWRDDVFEIAAAWIGDPIPEHNRTWYEVNGLGFA
                     HSASVQPVRDLPNLPLETIPYGGVLTEVSVPHVNAHWRPRTDSELAYRFYYGMTFWVT
                     GVSQDVSLQKWYRIFDDKYTYVYYAPAYAFRPIPVSELIPISSEVPPSEKLIEVDLAH
                     QWVNCYEAGQLVFTTKVSTGRKFGQDLYMTPSGEFETFRKRPSRHMAAGNLASGYDLP
                     GVPWVAYIDEEGVSFHGTFWHNDFGVPRSHGCINMTIDAAKWLYRWTNPVVPKHEQEV
                     WKGHGTPVRVA"
     gene            1176..1598
                     /locus_tag="VI520_00140"
     CDS             1176..1598
                     /locus_tag="VI520_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36320.1"
                     /translation="MAKHALFAGLVADPLGEPVEVATVGGEAFYVLDDDGFKRHIESE
                     AVDRQVLAKFAEFIRGNEDLVSEGTMKAIGQEDIFTKAIIDNSLKNLEHQFERLIETG
                     LPDEVRAWMGMLGFQVLIDAHGEVIEVRQPTGPENGDN"
     gene            1629..2465
                     /locus_tag="VI520_00145"
     CDS             1629..2465
                     /locus_tag="VI520_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF36321.1"
                     /translation="MTLSEYILDVSEATFETQVVLKSFDVPVVVDFWAPWCGPCRVLS
                     PILERLAIEAGGDFVLAKVNVDENPNLSIRYGVHGIPAVKAFRNGELAAQFHGAQPER
                     TVRKFIEQLTPSEAEQAVEQAHSLLATRHYPEAEAAYREVFEQDETNSEAALGLVESL
                     LIQGKGLEALSILQNFPPGTAWVRAEGYLPLASLLNEVEMAAPNLDLPPLEASLYQAG
                     RLIGRGNLPAAMDGLLDVLRQEKSYRAGLPKQVLLALFSLLGEQDPLTRKYRDELASV
                     LF"
     gene            complement(2545..>3145)
                     /locus_tag="VI520_00150"
     CDS             complement(2545..>3145)
                     /locus_tag="VI520_00150"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HLF36322.1"
                     /translation="PPTIDGDLADWTDPGRELGQIVFGASNWSGAGDASARFHIGWTE
                     QGLYLGLQVTDDKRVQVSSGRNMFKGDIVELQLDSDLTGDFFSNSLTSDDHQIGLSPG
                     NFGSLTSEAWRWYPIGSAGSLSGVSLKAVTTAEGYNLEAMIPWTTFGVTPDAGDRFGF
                     ALSISDNDLSGAPVQQSMLSTISTRTLTNPTTWGTLILK"
BASE COUNT          629 a          930 c          974 g          612 t
ORIGIN      
        1 ggctgtgtca acctcactat ccccgattca gagtggctat ataatttcgc ccccctcggg
       61 acgctggtga acgttcaccc gtagcggtcc gattcttgca gacattccgg gggaaggggt
      121 aattccgtgc cggacggaat cgaagctagc gctccactct cacgacggga attcgttcgc
      181 ctctcggcga ccggcctgct ggccttgggg attccagccc agaggacccg ggccgcggcc
      241 gaggccgagc cgggcctgct cggccgagta gtggatccga ctctggatat cttcagcggt
      301 ccctcctttg ccagcgagaa gcgcaagacc ctctggcggg acgatgtctt cgagatcgcc
      361 gcggcgtgga ttggagatcc aatcccggag cacaaccgaa cctggtacga ggtcaacgga
      421 cttgggtttg ctcattccgc ctccgtccaa cctgttcggg acctgccaaa cctgccattg
      481 gaaaccatcc cctatggagg agtgctgacg gaggtctccg tcccgcatgt caacgcccac
      541 tggaggccgc ggacggatag cgagctcgcc taccgcttct actatggaat gacgttttgg
      601 gtgaccggag taagccagga tgtcagcctg caaaagtggt atcgtatctt cgatgacaaa
      661 tacacctacg tgtactacgc accggcctat gcctttcgcc ccattcccgt gtcggaactg
      721 atcccgattt catccgaggt cccgccgagc gagaaactga tcgaagtgga ccttgcccat
      781 cagtgggtga actgctacga ggccggacag ctggtattca cgaccaaagt gtcgaccggc
      841 cggaaattcg gccaggacct gtatatgacg ccgagcggcg agttcgagac gtttcgcaaa
      901 cggccctccc gtcacatggc ggccggcaac ctggccagcg gctacgacct gccgggggtg
      961 ccatgggtcg cttatattga tgaagaaggg gtctctttcc acggcacgtt ctggcacaat
     1021 gactttgggg tgcctcgcag ccatggctgt atcaacatga ccatcgacgc cgcgaagtgg
     1081 ttgtaccgct ggaccaatcc agtggtcccc aagcacgaac aggaagtctg gaagggtcac
     1141 ggaaccccag ttcgagtcgc ctgagcatct acggtatggc gaaacatgcg ctgtttgccg
     1201 ggttggtcgc tgacccactc ggcgagccgg tggaggtcgc aaccgtcggc ggcgaggcgt
     1261 tctatgtgct cgacgacgac ggattcaagc gacacattga gagcgaagcc gtggaccgcc
     1321 aggtcctggc caagttcgca gagtttattc ggggcaatga agatctggtc agcgagggca
     1381 cgatgaaggc catcgggcag gaagatatat tcaccaaggc catcatcgac aactcgctca
     1441 agaacctcga gcatcaattc gagcggctga ttgagaccgg cctcccggat gaggtccggg
     1501 cttggatggg gatgctggga ttccaggtgc tgattgacgc tcacggtgaa gtcatcgaag
     1561 tccgccagcc gacgggcccg gaaaacggcg acaactgaag gtagaatcag agtaagtttg
     1621 cgatcggcat gaccctttcc gaatacatcc ttgacgtcag tgaggccacc ttcgagaccc
     1681 aggtcgtcct gaaatcgttc gacgtgccgg tggtggtcga tttttgggcg ccctggtgcg
     1741 gcccttgccg ggtgttgagc ccgatcctgg agcgcctggc cattgaggcc gggggcgact
     1801 tcgtcctggc caaggtcaat gttgacgaga atccaaacct ctcgattcgc tatggagtgc
     1861 acgggatccc ggcggtgaag gcttttcgca acggcgaact ggcggctcag ttccatggcg
     1921 cgcagcccga gcggaccgtc cgcaagttca tcgagcagct gacgccaagt gaagcggagc
     1981 aggccgtcga gcaggcccac agcctgctgg ccacccgtca ctatccagaa gccgaggccg
     2041 cctatcgaga ggtgttcgag caagatgaaa cgaactccga ggcggccttg gggctggtcg
     2101 agtccttgct cattcagggg aaagggttgg aagcgctcag cattctgcag aacttccctc
     2161 ctggtaccgc ctgggtgagg gcagagggat acctgccact tgcctcgctg ctcaacgaag
     2221 ttgagatggc tgccccgaat ctcgatctgc cgccgctcga ggccagcctg tatcaggcgg
     2281 gccggctgat cgggcggggg aatcttccgg cggcgatgga cgggctgctg gacgtcctcc
     2341 ggcaggagaa atcctatcgg gcgggcctgc ccaagcaagt gctgctggcg ctgttctcgc
     2401 tgctgggtga acaggaccca ttgacccgca agtacaggga cgagctcgct tccgtgctgt
     2461 tctaatcggg ccgaggaagc taaggggccg ggcgcatcgc ccggcccctt tttcttgttg
     2521 cgaatcacca agccgcggct gagcctattt caagataagg gtgccccaag ttgttggatt
     2581 ggtcagggtg cgggtgctga tggtggacaa catcgactgc tgaacgggtg ccccactcag
     2641 gtcattgtcc gaaatggaaa gggcaaaacc aaagcggtct cctgcatcgg gcgtcacccc
     2701 aaacgtcgtc caggggatca ttgcctccag gttgtaaccc tcggcggtgg tcaccgcttt
     2761 aagactcact cccgagagcg acccggccga gccaatcgga taccaacgcc aggcctcact
     2821 ggtcagcgaa ccaaaattgc ccggggagag cccgatttgg tgatcgtcgg acgttagcga
     2881 attcgagaag aagtcgccgg tcagatcgct gtcgagttgg agttcaacga tgtcgccttt
     2941 gaacatgttc cgtccgctgg acacctgcac ccgcttgtca tccgtaactt gcaatccgag
     3001 atatagcccc tgctcggtcc aaccaatgtg gaatctggcc gaggcatccc cggcgcccga
     3061 ccagttgctc gcgccgaaca cgatctgccc cagctcgcga ccgggatcgg tccagtcggc
     3121 aagatcgcca tcgatcgtag gtggg
//
LOCUS       DATDPQ010000009         5079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2880394, whole genome shotgun sequence.
ACCESSION   DATDPQ010000009 DATDPQ010000000
VERSION     DATDPQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880394"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VI520_00155"
     CDS             complement(<1..1092)
                     /locus_tag="VI520_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HLF36323.1"
                     /translation="MLKRLLHWLRRFFLPPAEAPLSIRLLPYGVLGALTFAGLVSGVY
                     VWDYTNSPQFCGTACHTMPPEYTAYLVSPHARIDCVECHIGRGFVATRVTRKAGDVRH
                     IIAQAFKHYEFPIQARDLRPARETCERCHFPAKFSDDSLREIKRFTPDAANTPVSIFL
                     SMKTGGGSEREGLGRGIHWHVENEVLYFSPDPHEQEIPLVRVVAANGIVTQYVDVESG
                     LDPESVDPILLKRMDCITCHNRITHLIPPPVDTVDELMARGLVPPAIPEIRLKAVEVF
                     HLDYESIEQAMAGIAELETYYQASHPEFYNADRPLLMRAIAALQEAYQASVFLEQRAD
                     WTSHPNNVGHKDAPGCFRCHDGKHLNQNQA"
     gene            complement(1110..2342)
                     /gene="nrfD"
                     /locus_tag="VI520_00160"
     CDS             complement(1110..2342)
                     /gene="nrfD"
                     /locus_tag="VI520_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HLF36324.1"
                     /translation="MNQALLIRKLPSRLGPALLAWAGLIGLLLLVGGVAALRVFRQGL
                     VLTNLSNSIPWGLWITIDLSAIALGAGAFTLSAIVYLFHLEHFRPILRLAVLVGLVGY
                     TTAMLTLLVDIGRPDRFWHPWVYWNVHSVLWEITLAITLYTAVMMVEFAPVVAELPLF
                     DQRPRLRRLGHWLHKAAPALALAGLLISIVHQSSLGATYGILKARPVWFKPSMPILFI
                     VSAVAVGPAVTMAAAFIVEWATRRPTIPHDLLRTIARISGIGLLVYAYLRFWDLAAVS
                     YYGRTPAAEAAMQLLQQYTPYGFGFWFGEVIIGLLIPVILFLLPRFNRRPGYLVLGAG
                     LAALGIVINRWNITVSGLLVPLNYSPGVVQQLEPGIYTPSLIEWTVVLGVVGYALAAF
                     TLGIRSLPIFTWHQTNPK"
     gene            complement(2383..3222)
                     /locus_tag="VI520_00165"
     CDS             complement(2383..3222)
                     /locus_tag="VI520_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLF36325.1"
                     /translation="MTEQRKKKVELKLTRRDFVKIAATAAGSAVAAQAVTTGPLSPFG
                     ELKRQQLRGEVRAPESAEGPRWGMVINLDTCIGCEYCQRACSAVNDVAPDKHWNIVQP
                     DQTETGRKFFFSRPCLHCQHAPCVEVCPVQATYVRDDGIVIMDYDRCIGCRYCEVACP
                     YDVRKFNWEARTDENAYVPTWGAPEIPRRPRGVVEKCTFCVHRIDAGMAQGLVPGRDR
                     EATPACVNICPVGARVFGDLNDPTSGVSRIVAEQPTLRLREELGTDSNVYYIPPKEET
                     VPA"
     gene            complement(3233..4300)
                     /locus_tag="VI520_00170"
     CDS             complement(3233..4300)
                     /locus_tag="VI520_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HLF36326.1"
                     /translation="MRILSSGPHRAPILLAVGFPVALFAAIGGLAARADSLPQLPAGS
                     GVPVQQGGDYAGPDTCGVCHEEIHTVWTDSRHANAFSSPIFQQNWAEIGSEFTCLTCH
                     TTGYDPTTNQFAFEGVTCEACHGPFQEGHPVEPMPIRADAELCATCHTTTKNEWDASK
                     HGQVGIQCQSCHNPHSQQPRAATITELCSNCHKERGETFTHGTHANAGLECSSCHMYT
                     DPLSGPPIQGLVPTGHTFAVGSQACIGCHQDTVHTRDTILKLSGEVAALEEVDLQALQ
                     QQLQQQEQAITDLEASRSARLYVGLAQGAIIGLATGGVTAWIISRRLRIVEPAAEPEP
                     QGPPSEPGSETPAGGTAGPTP"
     gene            4527..4754
                     /locus_tag="VI520_00175"
     CDS             4527..4754
                     /locus_tag="VI520_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36327.1"
                     /translation="MASSGWVVVKQGRCVRADREARLLEQRVYPAEFLPETVGYRVTA
                     LRCDCDVACNLQGLSCRWAFTLPEFDAFELA"
     gene            complement(4816..>5079)
                     /locus_tag="VI520_00180"
     CDS             complement(4816..>5079)
                     /locus_tag="VI520_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36328.1"
                     /translation="IKGLMAGASVTMMASELLLNGIQRIGEILSELELWLEVHEYESL
                     APLIGSMSVLRSGQSGAFERANYISVLRSYLPDAGLSALAEAS"
BASE COUNT          950 a         1519 c         1692 g          918 t
ORIGIN      
        1 cgcctggttc tgattcaggt gctttccgtc gtggcagcgg aaacatccgg gggcatcctt
       61 gtggccgacg ttgttggggt gggaggtcca gtcggcccgc tgttcgagaa agacgctggc
      121 ctggtatgcc tcctggaggg cggcgatcgc ccgcatgagc agcggccggt cggcgttgta
      181 gaactccgga tgggaggcct ggtaataggt ttcgagctcg gcgatcccgg ccatggcttg
      241 ttctatcgat tcgtagtcga gatggaagac ctcaaccgcc ttgagccgga tctctgggat
      301 ggccggcggc accaggccgc gggccatcag ctcgtcgacg gtgtccaccg gaggcgggat
      361 caaatgggtg atgcggttgt gacaggtgat gcaatccatc cgtttcaaga ggattgggtc
      421 gaccgactcg gggtctagcc cggattcgac atccacgtac tgggtgacga tgccattggc
      481 cgccactact cggaccaagg ggatctcctg ctcgtgcggg tccggcgaaa aatagagcac
      541 ctcgttctcg acgtgccagt ggattccccg gcccaacccc tctcgctcgg acccgccgcc
      601 ggtcttcatg ctgaggaaga tgctgactgg agtgttcgca gcgtcgggtg taaagcgttt
      661 gatttcccgc agactgtcgt ccgagaactt cgccggaaag tggcagcgct cacaagtctc
      721 gcgggctgga cgaagatcgc gggcttggat tggaaactcg tagtgcttga aagcttgggc
      781 gatgatgtga cggacatcgc ccgccttgcg ggtaacccgg gtggcgacga aaccgcggcc
      841 gatgtgacac tccacgcagt cgatgcgggc atggggtgag accaggtacg cggtgtactc
      901 cggcggcatg gtgtgacagg cggtgccgca aaactggggt gaattggtgt agtcccagac
      961 gtagacgccg gataccagcc cagcgaaggt gagggctccg agaacaccat agggcagcag
     1021 gcgaatgctc agtggcgctt ccgccggcgg caagaagaac cggcgcagcc aatgcaagag
     1081 tcgtttgagc attttgggca ggccggagtt tacttcggat tggtttggtg ccaagtgaag
     1141 atcggcaggc tgcggatccc gagggtaaac gctgcgaggg catatccgac gacccccagc
     1201 acgaccgtcc actcgatcag gcttggggta tagatccccg gctcaagctg ctgaacgact
     1261 cccggcgagt agttgagcgg gaccagcaat ccggagacgg taatgttcca gcgattgatg
     1321 acgatcccca gcgcggccag cccggcgccc aacaccaggt agccaggccg ccggttgaag
     1381 cggggcagga ggaaaaggat gactggaatc agcaagccga tgatcacttc tccgaaccag
     1441 aacccgaaac cgtagggagt gtattgctga aggagttgca tcgcagcttc ggctgcgggc
     1501 gtccgtccgt agtagctgac cgccgcgagg tcccagaagc gaaggtaggc gtacaccagt
     1561 aacccgatcc cgctgatccg agcaatcgtc cggagcagat cgtgcgggat tgtgggccgg
     1621 cgagtggccc actcgacgat gaacgctgcc gccatcgtaa cggccggccc gaccgcgacc
     1681 gccgacacaa taaacaggat cggcatgctc ggcttgaacc acaccggccg ggctttcagg
     1741 atcccatagg tcgcgccgag cgaggattgg tggacgatag agattagcag cccggccagc
     1801 gccagggcgg gggcagcctt atggagccag tgaccaagcc ggcgcaaacg tggcctttga
     1861 tcgaacaggg gcagttccgc gaccaccggc gcgaattcga ccatcatcac tgcggtatag
     1921 agcgtgatgg ccagggtaat ctcccacaag actgaatgca cgttccagta aacccaagga
     1981 tgccagaaac gatccggccg gccgatatcc accagcagcg ttaacatggc cgtggtgtag
     2041 ccgacaagcc cgaccagcac cgccaggcgc aggatcgggc ggaaatgttc caaatggaac
     2101 aggtacacga tggccgaaag ggtaaaagcc ccggccccca atgcgatcgc cgagagatcg
     2161 atggtgatcc acaggcccca cggaatgctg ttgctcaggt tggtaagaac caacccctgc
     2221 cggaatactc gcagcgcggc caccccacca accagcaaca gcagaccgat caggccggcc
     2281 caagccagca gcgccgggcc gaggcgagac ggaagtttgc gaatcaacag ggcctgattc
     2341 acgggttcga actcgccggt cgtggagcgt gggttgaacg cctcatgccg ggacggtctc
     2401 ctctttgggc gggatgtagt acacgttcga gtcggtcccc aactcctccc gcagcctaag
     2461 ggtgggttgc tcggccacga tgcgggaaac cccgctcgtc gggtcgttca ggtctccaaa
     2521 gactcgggcg cccactgggc aaatgttgac gcaggctggg gtggcttccc ggtctcggcc
     2581 ggggaccagg ccctgggcca tgccggcgtc gatccgatgc acacagaacg tgcacttctc
     2641 gaccactccc cgcggccgac gcgggatttc aggggcaccc caggtcggga cgtaggcgtt
     2701 ctcgtcggtc cgtgcctccc agttgaactt gcgtacgtcg tatggacagg ccacctcaca
     2761 gtaccggcag ccgatgcagc ggtcgtagtc catgatgacg atcccatcgt ctcgtacata
     2821 ggtcgcctgg accggacaga cctccacaca cggtgcgtgc tgacagtgca ggcacggccg
     2881 actgaagaaa aacttacgcc cggtctcggt ctgatccggc tggacgatgt tccagtgctt
     2941 gtcgggcgcg acgtcgttca ccgccgagca ggcccgctgg cagtactcgc agccgatgca
     3001 ggtgtccagg ttgatgacca tgccccagcg aggtccttcg gcggactccg gggcgcggac
     3061 ctcccctctc agttgctgcc gcttgagttc accgaaagga gagagcgggc cggtggtgac
     3121 cgcctgggca gccacggccg agccggccgc ggtggcggca atcttgacga aatcgcgccg
     3181 agtcagcttg agctcgacct tcttcttgcg ctgctcggtc accgcgtgcc tctcacgggg
     3241 tcggccccgc cgtgccgcct gccggggttt cagatccggg ttcgcttggc ggaccctgcg
     3301 gctccggttc ggccgccggc tcgacgatcc tcaggcgccg gctgatgatc caggccgtga
     3361 cgccgccggt ggccaagccg ataatcgccc cctgggccag cccgacatag agtcgggccg
     3421 agcggctggc ctccaggtct gtaatggctt gctcctgctg ctggagctgc tgttgcaggg
     3481 cctggaggtc gacttcctct agcgcagcca cctctccgct caatttcaaa atcgtgtcgc
     3541 gagtatggac ggtgtcctgg tggcagccga tgcaggcttg cgatcctacc gcgaaggtgt
     3601 ggccggtcgg gaccagaccc tggatgggcg ggccgctcag aggatcggtg tacatatggc
     3661 agctgctgca ctccaacccg gcgttggcgt gggtcccatg ggtgaaggtc tcgccgcgtt
     3721 ccttatggca attcgagcac agttcggtaa tggtggcggc ccgaggctgc tgcgaatggg
     3781 gattgtggca tgactggcac tggatcccga cttgaccgtg cttgctggcg tcccattcgt
     3841 tcttggtcgt cgtatggcag gtggcacaca gctcggcgtc ggcccggatg ggcatcggct
     3901 ccaccggatg accctcctgg aacgggccgt ggcaggcctc gcaggtcacg ccctcgaagg
     3961 cgaactggtt ggtcgtgggg tcgtagccgg tggtgtggca agtcaggcag gtaaactcac
     4021 tgccgatctc ggcccagttc tgctggaaga tcggagagga gaaggcattc gcatgccggg
     4081 aatccgtcca gacggtgtgg atctcttcgt ggcatacccc gcaggtgtcg gggccggcat
     4141 agtcgccgcc ttgctgaacc ggaactccgg aaccggccgg gagctggggc agcgagtcgg
     4201 cccgggccgc caggccaccg atcgcggcga agagcgccac tgggaaacca acggcgagca
     4261 agattggggc gcggtgtggt ccagaactca ggattcgcat gccggtcggc ggtcttccgc
     4321 ggctaaaagg atagtaatcg gctgaatggg accccatgag taccgtcgat caccgctaat
     4381 ttgtgatttt cgtcacgaac accgctacta attcgtagcg ctgatccgcg gtcagttgga
     4441 tggcattggt cactttcggc gggatcgccc ccggtttagg atggggaagg ggaaacagcc
     4501 tgcgacggtc ctttgccgga gggcgtatgg catcctcagg atgggtcgtg gtcaagcaag
     4561 ggcggtgcgt gagggccgac cgagaggccc ggctgctaga gcagcgggtc tatccagcgg
     4621 agttccttcc ggaaacggtg ggatatcggg tgaccgcctt gcgctgcgac tgcgatgtcg
     4681 cctgcaattt gcagggtctc tcctgtcgct gggccttcac cctcccggag ttcgatgcat
     4741 tcgagctcgc ctgaggcgag agtgcgggcg agctcctgaa cttgacacgc ctcccctggg
     4801 aggcgtgttc ttctatcagg aagcctcagc aagcgccgac agcccggcgt ccggaaggta
     4861 ggagcgtagc accgaaatgt agttggcccg ctcgaaggca cccgactggc cggagcggag
     4921 cacgctcatg ctcccaatca gtggggcaag cgattcgtat tcgtgcactt cgagccacag
     4981 ctcgagttcg ctcaagatct cgccaatccg ttggattcca ttcaggagca gctcggaggc
     5041 catcatcgtc accgaagcgc cggccatcag acctttgat
//
LOCUS       DATDPQ010000010         8329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_526584, whole genome shotgun sequence.
ACCESSION   DATDPQ010000010 DATDPQ010000000
VERSION     DATDPQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526584"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VI520_00185"
     CDS             complement(<1..828)
                     /locus_tag="VI520_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36329.1"
                     /translation="MPTREPRCPEPTEGTQLLTHEEDGYCFLYPDGLLRVDTSEHAVC
                     LVPEGDTQGCDNLVAAVEVTDAEGRTAAQIADEKMLSEGGFGADRCTAPIAGIQAVVL
                     PQVADQGLTCEMLFVREDRLYTLKFGLPDPDDPPEVGDALVHLFNTVTTSFTLPPVVA
                     APSPAKGTAVVAYVQEGDLRVWSEATGQTQILIDARDVIRVELSDDGELAAFVRRSYF
                     AAGGIDNNEQSALWVVGLDGSSPRELVSAAELRSNLDAAETDSTDFTRLEWIPNTHRL
                     "
     gene            complement(1694..2287)
                     /locus_tag="VI520_00190"
     CDS             complement(1694..2287)
                     /locus_tag="VI520_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36330.1"
                     /translation="MSRGFGFATIVLHVLAYVLRYLAAGFLTAAVLTTLTLLIVLYVA
                     VWGPEMEFLSILSPFLPIDAQGNARISGDDIMRAFSIMSLIMMGVAAALKAMIRKLGL
                     HSENRDDNQTRGRFMHGLGIPLIFITIVYAGSTLAMLLTGAARGPSEGGILSIMALTY
                     GIAMVSSLIYKAIDAFSQGILVTIQSGLDAGSIPGEP"
     gene            complement(2514..2957)
                     /locus_tag="VI520_00195"
     CDS             complement(2514..2957)
                     /locus_tag="VI520_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36331.1"
                     /translation="MKQDNDSSPVDPYQFYSEFLRKSTPVFIYVAVVVVSVLVFALDL
                     PFEILFAGMFFAMIAMGVSYARFVRSKAITSARSEEGTTYQMGDQKWLEPRAYFSRVA
                     IFLVASIAAILATFLVTHDAGIVGVLIAIVAIVWALLIRKGIRRA"
     gene            complement(3147..4793)
                     /locus_tag="VI520_00200"
     CDS             complement(3147..4793)
                     /locus_tag="VI520_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HLF36332.1"
                     /translation="MRTLVQSLQDHDRGYLRIVAELWGFDPPTGSRPAASQQLSEAML
                     AQASEMAASLPAEAARVLELLNRHAGRLPLEELGLRFGPLREMGPGRRDREKPWASPA
                     SPLEVLWYRGLLARAFADSPAGPQEYGFIPHDLASRLPTPTPNTPAPIGRPTARPRRF
                     AAADSFAVDDATTILAALRRRGWKVLELSPIRREWLLGHLLQPSSLDLLLNLLQQGGL
                     LAPEPQPELIREFLESSREAALSTLQRNYLRSAGWNDLAQVSSLRAAGPQWPNDPVAG
                     RRAAIELLASVPVGTWWSTPDLVAAAHQEVPHFLRPPGGFDSWYLQRATDGAFIRGIE
                     SWDSVEGELLSYLVGGPLHWIGAVDLSSDRGSFRWSPLGAPLRELRAQLEPTAANGRS
                     SVRPDGRVLVARLADRTLRYQVARLCEWESANRGSYAYRLTAGSLQGALGEGLTLGQA
                     QRILGEAAGGKPPKGILRAFERWAEHGQEAQVRPRLILEVRDAALLDQLSSHASTERF
                     LRERLGPTSAAVEPGDWEALSKAALRLGLLIGEPEKGSPP"
     gene            complement(4828..5742)
                     /locus_tag="VI520_00205"
     CDS             complement(4828..5742)
                     /locus_tag="VI520_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLF36333.1"
                     /translation="MERRVKLIFNPHADGGRGWRVAASLQATIEHFGGAEWTGTEFPT
                     HATQLAAQAAREGFEVVVAVGGDGTVHEVVNGLMLVTAAERPILAAVPIGSGNDFCHN
                     VGFAPDPGQAILSAFSGSTKTVDLGRVEDASGRVEYWDNTLGIGFDAATVINSHAITR
                     VQGLAMYLVAVIQTILRDHQAPRMKISTDREEIDRELLMLTLCNGGREGGGFMMAPDA
                     KPDDGVFEYVMVDYVSRAMMFRLIPEFMRGTHPRFRQVHLGSFRKLKLVADRPLLIHT
                     DGEVFANATTNVQELTVEVIPQALKVIV"
     gene            complement(6130..7968)
                     /locus_tag="VI520_00210"
     CDS             complement(6130..7968)
                     /locus_tag="VI520_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF36334.1"
                     /translation="MTSNPSPILINRRTLVFLFGMALTQFSIAMPLGQIPVHIRELGA
                     SISQIGLFFTISMVFPLVLKVLGGWLADSLGRLRVIFIGSLTGVLTFAAYALAPSWQA
                     ALLGPAFMAMTSALTLPAYFAFIADITPEGSRGRMYGISQTIYHAAAVIAPPIGGLLA
                     QTYGYRFLFGVSSAVFGLAAVIFIYLLRFSQFMTTRQQEISLRSLGSSLSQMAGAFMA
                     GGVVAWILIVDGFRDVVFKLSADLEPVYLTEIAGISKQGIGLLDGIWGAALLLTLFPA
                     GWLVDRTSERLGIVLALLAAIASRLVFALATGFWGFALCWSLSGVGVGLFEASGPKLI
                     TRVIPKHLRGTAFGLLASTLSLFSLPAPWIGSQIWNLAGPRWPFLVTAALGSLTLLPA
                     WFKLALPAEPRAASRAAEAATLPTIGHSEVATVLFARFPPSTESSVHDQGKQILLEHG
                     AVLDSGMDNVLIAWFGVSPRRSPPQVSALLATHAGLSLLAAVTGVNPDRAAAGLAELT
                     IDIGIATGDLTTSPGPPEYQHDQGVRSGEEVTVMGDVLRAAERLQSYGPHSGGVLISE
                     RTYLALGPALHQFVFGRSGPVRLPGEDGESRAHEVLGRAQPLGPAG"
     gene            complement(7965..>8329)
                     /locus_tag="VI520_00215"
     CDS             complement(7965..>8329)
                     /locus_tag="VI520_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLF36335.1"
                     /translation="DFWRGEVLDLELVGYIRGLKPNYRTGLISNAWREIRQDIENEWR
                     IADAFDDLIISAEVGLAKPDPRIYQLALKGLGVEASQAIFVDDFIENLEAASAQGMHP
                     VHFKNRTQAQQQVETLLA"
BASE COUNT         1660 a         2603 c         2589 g         1477 t
ORIGIN      
        1 cagacggtgg gtgttgggga tccactccag ccgcgtgaag tcggtggagt cggtctcggc
       61 ggcgtcgagg ttggatcgca gctctgccgc cgagaccagc tcgcgcgggc tcgagccgtc
      121 caggcccacg acccacaggg cagattgttc gttgttgtcg atcccgccgg cggcgaagta
      181 cgatcggcgt acgaacgccg ccagctcgcc gtcatcgctc agttccaccc ggatcacatc
      241 gcgggcgtcg atgaggatct gagtctgacc ggtcgcttcg gaccataccc gaaggtcgcc
      301 ttcctggaca taggcgacca cggctgttcc tttcgccggg ctcggagctg caacgacggg
      361 cggcaacgtg aacgacgtgg tgaccgtgtt gaagaggtgt accagggcgt cgccgacctc
      421 gggcgggtcg tcgggatcgg gcaaaccgaa cttcagcgtg tacaggcgat cctcccgcac
      481 gaacaacatt tcgcacgtaa gcccctgatc ggcaacctgg ggtagaacca cggcctggat
      541 gccggcgatc ggtgcagtgc aacggtcggc cccgaagccg ccttcactga gcatcttctc
      601 gtcggcgatc tgggcggcgg tccttccctc ggcgtcggta acttcgacgg cggcgacgag
      661 attgtcgcat ccctgggtgt ccccttcggg gacgaggcac accgcgtgct cggaggtatc
      721 cacccgaagc agtccgtcgg gatacaggaa gcagtagcca tcctcttcgt gcgtgagcaa
      781 ctgcgtgccc tccgtgggct cggggcagcg cggctcgcgg gtcggcaagg gcgggggagt
      841 cggagatgcg gcgggaggag ccgaggtctc agtgggtatg accaaaggag acgccgacgg
      901 ggtgcaggcc gcagccagca gccccagcgc gatcactgcg ggaaccagtc gagatctccg
      961 agaagggcgt tgaccgtcca ttgggcctcc atgggtggat caccgtcgtc acggtaagtc
     1021 taaccgcagg agctcgttcg gctcagtcgg caatcgaggc cagggagcgg tgaaggggat
     1081 gatgcggagc gggggctggg accccgaccg gccgacggtg gctaagtatg ccccaagacg
     1141 gcgcagaata cacgacgtgc cgacctgcaa acctggtttg ctctgcaaga cgggtttgtc
     1201 cctgccaagc cgctggcgaa ccctgagctg acgtggatac ccgccttcgg tgcgggctca
     1261 ccgatacttc ttctcaatct ccgcgaagag ggccctgaag agccggacaa attcggactc
     1321 actcagcttg actcgtcgcc cgctttgagc cccaggaccc acatctccgt cgggagcttg
     1381 atcccacttt gtttcgattg gcacacctgt gatagtgggc ccgtgaggtg aataaacgat
     1441 agcccaagta cctgctgcct aacggtcgcg agctgagcgg cgccgcttag ctacttcgga
     1501 cattatctcg cgccgtggcg gcatccgtgc atcctgcctg cacctgggca gcaggcatag
     1561 gtgagagcga agcgatcagg gctccagcga ggggttagga agcgccgcga tccacgattg
     1621 gaatgtcact aggccttttg gcgacattcg gcggcaaatc ggatccgcaa acatttacgc
     1681 attcgagatc aattcatggc tcacccggga tcgaccctgc atccaaccct gactggatcg
     1741 tcacaagaat gccttgcgag aaggcatcaa ttgccttgta gatcaggctt gataccatgg
     1801 ctattccgta cgtcagtgcc attatgctga ggattccccc ttcggatggg ccccgcgccg
     1861 caccagtgag cagcatggct agtgtcgaac ccgcatacac aatggttatg aatatgagcg
     1921 ggatccccaa gccgtgcatg aatcggcctc tggtctgatt gtcgtctcgg ttctcggaat
     1981 gcagcccaag tttccggatc atggctttca gcgccgcggc aacgcccatc atgatcagac
     2041 tcattataga gaaggcgcgc atgatgtcgt ctccgctaat ccgagcgttg ccctgggcgt
     2101 cgatagggag aaacggggac aggatgctga ggaactccat ctctggcccc cagacagcta
     2161 cgtagaggac aatcagcaag gtgagtgtgg tcagcactgc ggctgtgagg aacccggctg
     2221 ccaaatacct caacacatag gctagcacgt gcaacacaat ggtggcgaac ccgaagcctc
     2281 tactcaattg gatcagttct ccgttgttgg agaccgactc aatacacagc gctttctaac
     2341 ggcctcgagc tgagcggcgc agcaaagctc ctgcagacat catagcgcgc cctggctgcg
     2401 tccgtgcatc ctgcctgcac ttgggccgca ggcataccat cctggcacag ggcaggtgag
     2461 agcgaagcga tcagggctcc agcgaggggt tagctggact gtcactcgat gtttcaggct
     2521 cttcttattc cttttcttat cagaagagcc cagacgattg ccacaatcgc aataagtact
     2581 ccaactatgc cggcatcatg cgtgaccaga aatgtcgcca aaattgctgc tatcgatgcc
     2641 actaagaaaa tagcgacgcg cgaaaagtac gctctgggct ccaaccactt ttgatctccc
     2701 atctgatacg tagttccctc ctcgctcctt gcagaggtaa tcgcttttga tctcacaaag
     2761 cgagcataag aaacgcccat agcaatcatc gcgaagaaca tccctgcaaa gaggatttca
     2821 aagggcaaat ccagtgcaaa gacaagaacg gaaactacga caactgcgac ataaatgaag
     2881 acgggtgtgg actttcgcag aaactcggaa tagaactgat atggatcgac gggcgaacta
     2941 tcgttgtctt gtttcatcca ctgtatccat tcaggccagc taactcgtga ttatgtggaa
     3001 acagttcccc atatcccccg tgcaggggat gtttcgggga agttattccg ggatttgtcc
     3061 gactggctgt attatgggga aaagtttccg ctttgtctac tgagtcgcgt ggataggtga
     3121 acggaagccc ccctcgctcc ttgtatttaa ggaggcgacc ccttctcagg ttcgccgatt
     3181 agcagcccga gcctcaaggc tgccttgctc agcgcctccc aatcgcccgg ctcgacggcc
     3241 gcactcgtcg ggccaagtcg ctccctcaag aatcgctcgg tcgaggcatg ggaactcagc
     3301 tgatctagca gtgcggcatc ccgaacctcg aggatcagcc gcgggcgaac ttgcgcctcc
     3361 tggccgtgct ccgcccagcg ctcgaaggcc cgtagaatcc cctttggagg tttgccgccc
     3421 gcagcttccc ccaggatccg ctgggcttgg cccagagtca ggccctcccc gagcgctccc
     3481 tgcagcgagc cggcggtgag ccggtatgca tagctgcccc gatttgcgct ctcccactcg
     3541 cagaggcggg cgacctgata tcggagggtc cgatcggcta accgggcgac cagaactctg
     3601 ccatccggcc gaacggacga ccgcccgttg gcggcagtcg gctccagctg agcacgcagt
     3661 tcgcgcagcg gcgctccgag cggactccag cgaaacgacc cccggtccga actcagatcg
     3721 accgcgccga tccagtgcag tgggccgcca accaggtagc tcagcagctc cccttctact
     3781 gaatcccagg actcaatgcc ccggatgaag gctccgtccg ttgcgcgctg caggtaccag
     3841 gagtcaaagc cccccggagg tcgaagaaag tgagggactt cctggtgggc agccgcgacc
     3901 aggtcggggg tggaccacca ggtcccaacc ggcacgctgg ccaaaagctc aatcgctgct
     3961 cgccgccccg cgaccgggtc attgggccac tgtgggccgg cagcgcgaag cgagctcacc
     4021 tgcgcgagat cgttccagcc ggcagaccgc aagtagttcc tctgaagggt ggacagggcc
     4081 gcctcccgac tgctctcgag aaactcccgg atcagttctg gctgcggctc gggagccagc
     4141 aatccaccct gctgcagcag gttcagcagc aggtccagcg agctgggctg cagcaggtgc
     4201 ccgagcagcc actcccgacg gattggggag agctccaata ctttccagcc gcggcggcgg
     4261 agggcggcca ggatcgtcgt ggcatcatcg accgcgaagc tgtcggccgc cgcaaaccgg
     4321 cggggccttg ccgtcggccg accgatcggt gccggggtgt tcggggtggg cgtcggcagt
     4381 ctgctggcca ggtcgtgcgg gatgaacccg tactcctgcg gacccgccgg cgagtcggca
     4441 aatgctcgag cgagcaggcc gcgataccag agcacttcca gcggcgaggc cggggaggcc
     4501 catggcttct cccgatcgcg ccgacccggg cccatttcgc gcagcggacc aaatcggagc
     4561 ccgagttctt cgagcgggag acggcctgcg tggcggttga gcagctccag cacccgggcc
     4621 gcctcggcag ggagggaggc ggccatttcg gaagcctggg cgagcattgc ctcagagagc
     4681 tgctgggagg cggccggcct agagccggta ggcggatcga agccccatag ctcggctacg
     4741 atacgcagat agccgcggtc atggtcctgc aggctctgaa ctaaggtcct cattcgaacg
     4801 gagcccgatc gttggggatc gggggcccta gacgatgact tttagcgcct gggggattac
     4861 ttctaccgtc agttcttgaa cgttggtcgt ggcattggcg aagacctcac cgtcggtatg
     4921 gatcagcaat ggccgatcgg cgaccagctt gagcttgcga aaggatccaa gatgaacctg
     4981 cctgaagcgg gggtgggtgc cgcgcatgaa ttcgggaatc aggcgaaaca tcatggctcg
     5041 ggagacgtag tcgaccatga cgtactcaaa cacgccatcg tcgggcttgg cgtcgggggc
     5101 catcatgaat ccgccgccct cccggccgcc gttgcacagg gtcagcatga gcagctctcg
     5161 gtcgatctct tctcggtcgg tcgagatctt catccggggt gcctgatggt ccctaaggat
     5221 cgtctggatg accgccacca gatacatcgc cagaccctgt acccgagtga tggcgtggga
     5281 gttgatcacg gtggccgcgt cgaacccgat ccccagggta ttgtcccaat actcaacccg
     5341 tccgctcgca tcttccaccc gaccgaggtc cacggtcttg gtggagccgg aaaaggccga
     5401 gaggatcgcc tgacccggat cgggagcaaa accgacgttg tggcagaagt catttcccga
     5461 gccaatcggc actgcggcca ggattggtcg ttcggcggcc gtcaccagca tcaatccgtt
     5521 gacaacctcg tggaccgtac catctcctcc caccgcgacc accacctcaa acccctcccg
     5581 ggccgcctgg gcggcgagct gagtggcatg ggtcgggaac tcggtgccgg tccactcggc
     5641 tccaccgaaa tgctcgatcg tggcctgcag ggaggccgcc actcgccagc cacgaccgcc
     5701 gtcggcgtgg ggattgaaga tcagcttgac tcggcgctcc atgttgccgg ccttcaatgt
     5761 gcggggttca gcggagacac ttgcagcgga ttatagccca cccctcgcgg tagtaccttt
     5821 tttaagcgat tcgatcattg actctctgcc caagcctgct acagtgagcg agtaggagcc
     5881 tcaggccacc aacggactgc aaccccccgc gtcggccgtg gcgctctccg accaatcagc
     5941 ccaagccatt cagtggatgg cgacgggaaa gcaacacgcc ggtcggtcgg agtcccgccc
     6001 ttccctgcgg cgtatagatg tggggccttc cggcaagagc ggcagtgccg gccgtccagc
     6061 gaatggcagg gggctcttcg ttttaaggac ttcgctggtt gaaaagcttg atcactccgc
     6121 gagcgggggc taaccggcgg gtccgagcgg ttgggcccgc ccaagcacct cgtgggcccg
     6181 cgactcccca tcttcgcccg gtaggcggac cgggccgctc cgaccgaaca caaattgatg
     6241 cagcgcggga ccaagcgcga gataggtgcg ctcgctaatc agcacgccgc ccgaatgcgg
     6301 cccatacgac tgcagccgct cagccgcccg caacacgtcg cccatcacag tcacttcctc
     6361 cccggaacga accccttgat cgtgttggta ttccggaggg cccgggcttg tcgtcagatc
     6421 gccggtcgca atcccaatgt cgatggtgag ctcggccagg cccgcggcgg cccgatccgg
     6481 attgaccccg gttactgcgg ccaagagcga gagcccggcg tgggtggcca gcagcgcgct
     6541 cacctggggc ggggaccggc ggggggaaac cccgaaccag gcgatcaata cgttatccat
     6601 ccccgagtcc aggaccgcgc catgttccag gagaatctgc ttcccctggt cgtggaccga
     6661 gctctccgta cttggcggga acctggcgaa caacacggtg gcgacctcgg agtgtccgat
     6721 cgtcggcaag gtggcggctt ccgccgcccg acttgcagcc cgcggctccg ccggcagcgc
     6781 cagtttgaac caggccggca ggagggtcag actgccgagg gcggccgtca ccaggaaagg
     6841 ccagcgcggg ccagcaaggt tccaaatctg gctgccgatc cacggtgctg gcagcgaaaa
     6901 gagactcaaa gtcgaggcga gcaatccaaa agccgtgccg cgcaggtgct ttggaatgac
     6961 tcgagtgatc aacttgggtc cagaagcctc gaacaggccg acgcccactc ctgatagcga
     7021 ccaacacaag gcgaatcccc agaatcctgt ggccagcgcg aacaccaggc gggaggcaat
     7081 agccgccagg agcgcgagca cgatgcccag ccgctcgctg gtccggtcca ccagccaacc
     7141 ggccggaaac agggtcagca gcaaggccgc cccccagata ccgtccaaca ggccgatccc
     7201 ttgcttgctg atccccgcga tttccgtgag gtagaccggc tccaggtccg cggagagctt
     7261 gaagacgacg tcgcggaaac cgtccacaat tagtatccag gccaccactc ctcccgccat
     7321 gaaggcgccc gccatctggc tcagggagct gccgagtgag cgcagcgaga tctcctgttg
     7381 tcgggtggtc atgaattgcg agaaccgcag cagataaatg aagatgacgg ctgccagacc
     7441 gaacaccgca ctcgacactc cgaataagaa tcggtagccg taggtctggg ccaacaaccc
     7501 gccaatcggt ggcgcaatca cggccgcggc gtggtagatg gtctgggaga tcccgtacat
     7561 cctgccccgc gagccttctg gagtaatgtc ggcgataaag gcgaaatagg cgggaagagt
     7621 gagggcggag gtcatcgcca tgaatgccgg acccagcagc gcggcctgcc agctgggtgc
     7681 caaggcatag gcggcgaagg tcaggacccc ggtcaggctg ccgataaaga tcaccctcaa
     7741 tcgcccgagc gaatcggcca gccagcctcc gagcaccttc aacaccagcg gaaataccat
     7801 ggagatcgtg aagaacagac cgatctgcga gatgctggcg cccagttccc ggatgtgcac
     7861 tggaatctgg ccgagcggca tggcgatcga gaactgggtc agcgccattc cgaagaggaa
     7921 gaccagcgtg cgacggttga tcaggatggg gcttgggttg gatgtcacgc gagcagcgtc
     7981 tcgacctgtt gctgggcctg ggtccgattc ttgaaatgga ctgggtgcat tccttgtgcg
     8041 gaggcggcct caaggttttc gatgaaatca tcgacgaaga tcgcttgact cgcctccacc
     8101 cccagaccct tgagtgccag ctgataaatg cgcgggtctg gtttggccag ccccacctca
     8161 gcggagataa tcaggtcgtc gaaggcatcg gcgatccgcc actcattctc gatatcctgg
     8221 cggatttccc gccaggcatt ggagattaat ccggtgcggt agttcggctt caatccgcgt
     8281 atgtacccga ccagctcgag atcgaggacc tctcccctcc agaagtcct
//
LOCUS       DATDPQ010000011         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_265347, whole genome shotgun sequence.
ACCESSION   DATDPQ010000011 DATDPQ010000000
VERSION     DATDPQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265347"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="dnaN"
                     /locus_tag="VI520_00220"
     CDS             complement(<1..714)
                     /gene="dnaN"
                     /locus_tag="VI520_00220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLF36336.1"
                     /translation="MKLSVLQENLSQGLTTVARAVSPRSTLPVLANVLVATEDGRLRL
                     SGTNLELGITCWIGAKVTEEGATTVPARTFVDLVNTLPADRVEMELTLRTQTLNVRAG
                     AFDNNIKCIDAQEFPPMPPADLGDGVPLEMQDLREMISQVTFAASSDDARPVLTGVLV
                     EIDGTHLTLAAADGFRLSVRRAELTAPVSSPVRAIVPARALAELARIASDEQVHMLLP
                     PGRGQVIFRGRDLELVSQLI"
     gene            792..2045
                     /locus_tag="VI520_00225"
     CDS             792..2045
                     /locus_tag="VI520_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLF36337.1"
                     /translation="MAVQELQQELDWIRELVFQRSRLLEISVTLNSTLELNRLLQFIM
                     DSTADLVDSEGASILLVDENTKDLFFAAATGTNPDELRRIPVPMEGSIAGTVFKEDRP
                     LIINEVAQDPRHFQQVEEKTKLKTRSLAAVPMRMRDEVIGVLEAINKRNGAFEKRDLE
                     TLGIVASQAAVAINNARLLNALKKAYEELGKLDRMKSDFIAIASHELRTPLGVILGYA
                     ALLKEEAGGESSEHAETVLNMAMRMRSLIEDMTNINAMKVGSHELALEIQPLQVPIRT
                     AQAEVADLIQAKGQRLMVELLGEPLQARIDAAKLSMALSNLLNNALQFAPPKGEIRLK
                     LERHGSEGWIRVTDNGIGIPAGELEQIFGQFYQVEHHMVRKHQGMGLGLSIVKAIAEA
                     HGGRVWAESAGPGQGATFTIALALT"
BASE COUNT          442 a          659 c          724 g          384 t
ORIGIN      
        1 aatcaattgc gagacgagtt ccaggtcccg accgcggaaa atgacctggc cccggccggg
       61 cggcagcagc atgtggacct gctcgtcgct cgcgatccgg gccagttcgg ccagcgcccg
      121 ggccggcacg atagcccgca ccgggctgga gaccggcgcc gtgagctcgg cccggcgaac
      181 cgagagccgg aacccgtcgg ctgcggcgag ggtcagatga gtgccgtcaa tctccaccag
      241 cacgccggtc agcaccggcc gggcgtcgtc gctggaagcc gcgaaggtca cctgggagat
      301 catttcccgc aggtcctgca tttcgagcgg gacgccgtcc ccgaggtcgg cgggcggcat
      361 cggcggaaac tcttgggcgt caatacactt gatattgttg tcgaaggcgc ccgcccggac
      421 attgagggtc tgagtccgga gggtgagctc catctcgacc cggtcggccg ggagggtatt
      481 gaccaggtcc acaaacgtcc gagcgggcac ggtcgtggcg ccctcctcgg tgaccttggc
      541 tccaatccag caggtgatcc ccagctccaa attggtgccc gaaaggcgca gccggccgtc
      601 ttcggtggcc accagcacgt tggccaacac cggaagcgtg ctgcggggcg agacggcccg
      661 agcgacagtg gtcagccctt gagagaggtt ctcttgcagg acggagagct tcatcttaag
      721 tgcaatcgcg aagtataccc tgcggtaatg gttttgtaga caagcctgcg gctctcgaat
      781 acacttgcgc agtggccgtc caggaactcc agcaggagct cgattggatc cgcgagctcg
      841 tatttcagcg ctcccggctg ttggagatca gtgtcaccct caattccacc ctcgagctga
      901 accggttgct gcagttcatc atggattcga ccgccgactt ggtggacagc gaaggcgcct
      961 cgatcctgct ggtggacgaa aacaccaaag acctgttctt cgccgcggca accgggacca
     1021 acccggatga gttgcgccgg attccggtgc caatggaagg ctcgattgcc ggcacggtct
     1081 ttaaggaaga ccggcccttg atcatcaacg aagtggctca ggaccctcgc cacttccagc
     1141 aggttgagga gaagaccaaa cttaagactc gctcgctggc ggccgtgccg atgcggatgc
     1201 gggacgaggt gatcggggta ttggaagcga tcaacaagcg caacggggcc tttgaaaagc
     1261 gtgacctgga aaccctgggg attgtcgcct cgcaggcggc agtggcgatc aacaacgccc
     1321 ggctgctcaa cgcactcaag aaggcctatg aggagctggg taagctggac cggatgaaaa
     1381 gcgatttcat cgcgattgcc tctcacgaac tgcgcactcc gctgggagtg atcctgggtt
     1441 acgcggcgct gctcaaggaa gaggccggcg gggaaagctc cgaacatgcc gagaccgtgt
     1501 tgaacatggc gatgcggatg cgtagcctga tcgaggacat gaccaatatc aacgcgatga
     1561 aggttggctc gcacgagctc gcccttgaga tccagccgtt gcaagtgccg atccggacgg
     1621 cccaagccga ggtggccgat ttgatccagg ccaagggtca gcggctgatg gtcgagctgt
     1681 taggggaacc gctgcaggca cggatcgacg cggccaagct gagcatggcc ctctccaact
     1741 tgctgaacaa cgccttgcaa ttcgcgccgc cgaagggcga gatccggctg aaactggaac
     1801 ggcacggatc ggagggctgg atccgggtga ccgataatgg gatcgggatt ccggccggcg
     1861 agctagagca gatctttggc cagttctatc aagtggaaca tcacatggtt cgcaagcacc
     1921 aggggatggg actggggctc tcgatcgtca aggccatcgc cgaggcgcac ggcggccggg
     1981 tgtgggccga aagcgcgggg ccggggcaag gggcgacttt tacgatcgcc ctggctctaa
     2041 cctagcgaca actcaactct cgccagcggg ccacaacaac gcatccccct tgcgggcggg
     2101 ttgatctacc ttaatggcga cttccttccc ggccatcaca tcggtgaccg gctggttctc
     2161 gacgtgcatc gagctgatct cctggacaaa gtcggtcttc gtgccgagg
//
LOCUS       DATDPQ010000012         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2880895, whole genome shotgun sequence.
ACCESSION   DATDPQ010000012 DATDPQ010000000
VERSION     DATDPQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880895"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..547
                     /locus_tag="VI520_00230"
     CDS             239..547
                     /locus_tag="VI520_00230"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HLF36338.1"
                     /translation="MSDPASMKEVNMMGGGMMGGYGFGSFGLIGMILNLVITVGLIVG
                     IVLLVAWLVRRLGSQGGAFSAGQQPGQAAIAPREILQARYARGEIKRDEYQQMLSDLG
                     "
     gene            618..848
                     /locus_tag="VI520_00235"
     CDS             618..848
                     /locus_tag="VI520_00235"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HLF36339.1"
                     /translation="MMGFGMGIGLLVLLLFWGLLIFGAAWLVKGVFSGDKRTLGASVG
                     SGAKPREILDQRYARGEIAREEYDRIRQDLGL"
     gene            complement(983..1174)
                     /locus_tag="VI520_00240"
     CDS             complement(983..1174)
                     /locus_tag="VI520_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HLF36340.1"
                     /translation="MARDLVCGMDVKEATAQWKSEYQQQMYYFCSPGCKKAFDKNPEK
                     YVTRASMHGEHSQHGHEHH"
     gene            complement(1233..1583)
                     /locus_tag="VI520_00245"
     CDS             complement(1233..1583)
                     /locus_tag="VI520_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36341.1"
                     /translation="MESLRTVSMDCDETADLLSRSFAAMGLRVTRSFDLLSARQAMRN
                     PEGCPCPHHGTASCSCQYVVLLVENAAGSLLTLVAHGHDGATHLSMDQLSLDPTVRDV
                     VQQLIVGLRSAAPG"
     gene            complement(1717..>2566)
                     /locus_tag="VI520_00250"
     CDS             complement(1717..>2566)
                     /locus_tag="VI520_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLF36342.1"
                     /translation="TLLALWFGARQVIRPLRRLQQQAEELTESEYPRLADPVGGIAEI
                     RQLQASLSTMAARIQAAHQALQSYIGSITLAQEEERRRLARELHDETIQDLIAIDHRV
                     QILSQGLQAGDGEVATRLDGLRREVNRSIQELRRLIGALRPIYLEDLGLVPALGMLTQ
                     EAARDLGVNVSFDVEGHPRRLSPEAELAVFRIVQEALNNVGRHAKATKAFVRLRYGQD
                     KVVTQIQDDGDGFVPTERFGDLASLGHFGLLGMHERAQLAGAHVSLASEPGKGTLISI
                     ELPLPA"
BASE COUNT          459 a          779 c          824 g          504 t
ORIGIN      
        1 gggcaggagg cggaagcggg atcggcctga ccatcgccaa gcacctggtg gaagctcatg
       61 gtgggcagat ctgggccagc agcccgggat ccggcaaagg aagcagcttc acgttcacgc
      121 taccgctcgc cggctgagcc taactgcgcg gatctttacc aaaggtttaa cagggcaaca
      181 aattcccttc aaaagcggct gatacgatcc cagcaagtct cgaggccaca acaaccgctt
      241 gagcgacccg gcctcgatga aggaggtcaa catgatgggt ggtggaatga tgggaggtta
      301 cgggtttggg agtttcgggc tgatcggcat gatcctcaac ctggtcatca ccgtaggcct
      361 gatcgtcggc atcgtgctgc tggtggcctg gctcgtgcgc cggcttggct cgcagggtgg
      421 agccttctcg gcaggccagc agccagggca ggcagccatt gcgccgcgcg agatcctgca
      481 ggcgcgatac gcccgcggcg agatcaagcg cgacgagtat cagcagatgt tgtctgacct
      541 aggttgattg cagctctagg tccggggcgg tccgctgcac cggggccctg agtggctcaa
      601 tcgtcggaga aaacaccatg atgggattcg gaatgggaat cgggctcttg gtcctactgc
      661 tgttctgggg cttgttgatc ttcggggcgg cttggctcgt caagggggta tttagcgggg
      721 acaaacggac tctcggggcc tccgtcggaa gcggcgcgaa accgcgtgag attctggatc
      781 aacgctacgc ccgcggcgag atcgcccggg aggagtacga tcggatccgc caggacctcg
      841 ggctgtagtc cgcgcagcgc gagcaagggg cccagggtgg aagcgacacc tgggcccctt
      901 tgtttagctc ctacgaccct aatacccggc tgggcgggac ccacttggtt cacccgcggt
      961 cctttggggc tcgatcctcg attcagtggt gttcgtggcc gtgctggctg tgttcgccgt
     1021 gcatcgatgc gcgggtcacg tacttctcgg gattcttgtc aaaggccttc ttgcaccctg
     1081 gcgagcagaa gtagtacatc tgttgctggt actccgattt ccactgcgca gttgcctcct
     1141 tcacatccat gccacagact aggtctcttg ccatcgttca agcctccttt ccccagctgc
     1201 cgaatggctg gaattcaagt tcggcgtcga ccttatcctg gggcagccga gcgcaacccg
     1261 acaatgagct gttgtacgac atcgcggacg gtcggatcca agctcagctg gtccattgaa
     1321 agatgggtcg ccccgtcatg cccatgcgcc acaagggtca agaggctgcc cgccgcattc
     1381 tccaccaaca gaacgacgta ctggcaggag caactcgctg tgccatggtg ggggcaaggg
     1441 cagccctccg gattcctcat cgcctggcgg gcagacagca ggtcgaaaga acgcgtcacg
     1501 cgtagcccca tggctgcaaa actgcgtgag agtagatccg cggtttcgtc acagtccatt
     1561 gagacggttc gcaggctctc catcgtgtgc tctaactgcc attctagagt gcaggcccgg
     1621 tctcactgag ggtgatatgg ctgcgcctcc gataggctga tcggcgggtg ccgccgtgtg
     1681 aacagctcgg ccccgcggcg acggctgcct gctggcctat gccggcagag gtagctcgat
     1741 ggaaatcagc gtgcccttcc caggttcaga ggccaggctc acgtgcgccc ccgccagttg
     1801 ggcccgctcg tgcattccca gtagtccgaa gtgcccgagg gaggccaggt ccccgaatcg
     1861 ctcggtaggc acaaagccgt ccccgtcatc ctggatctgt gtgacaactt tgtcttgccc
     1921 atagcggagc cgtacgaacg ccttggtggc cttggcgtgg cggccgacat tgttgagggc
     1981 ctcctgcaca attcgaaaaa cggctaactc ggcctcggga ctcaggcggc gcgggtgacc
     2041 ttcgacgtcg aaactcacat tcactcccag gtcgcgcgct gcttcctggg tcagcattcc
     2101 gagcgccggc accaggccca ggtcttccag atagatcggc cgcaaggcgc cgatgagccg
     2161 gcgaagttcc tggatggagc gattgacctc gcggcgcagg ccatccagcc gagtggccac
     2221 ctcgccatcg ccggcttgca gaccttggct taggatctga actcggtgat cgatggcgat
     2281 caagtcttgg atcgtctcgt cgtgcagttc gcgggccagc cgacgccgct cctcctcctg
     2341 ggcaagggtg atcgagccaa tatagctctg cagcgcctga tgagctgcct gaattcgggc
     2401 ggccatggtg ctcagtgaag cttggagctg ccggatttcg gcgatcccac ccaccgggtc
     2461 cgccagcctg gggtactcgg attcggtcag ttcttcggct tgctgctgga gcctacgcag
     2521 cgggcggatg acctggcgcg caccgaacca cagcgccagc agggtg
//
LOCUS       DATDPQ010000013         6408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1313601, whole genome shotgun sequence.
ACCESSION   DATDPQ010000013 DATDPQ010000000
VERSION     DATDPQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313601"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VI520_00255"
     CDS             <1..438
                     /locus_tag="VI520_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HLF36343.1"
                     /translation="SSTSDPSCSPSGNTGFENTLLGLINQERAAQGLPAYAAQGQLQT
                     AARLHSTDMGCNGFFSHTGSDGSTVGLRVTAQGYSWSWVGENIFAGTSATPQSAFDWW
                     MNSAPHRANLLSPNYTEIGLGYIYEAGSPYGGYFTAVFAKPSS"
     gene            475..1581
                     /locus_tag="VI520_00260"
     CDS             475..1581
                     /locus_tag="VI520_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HLF36344.1"
                     /translation="MRRAVFMVSPELWERGHGPQHPLKPERLQRTFELLSEYGAFAAP
                     NVQIVKPRLAEPDELALFHTQDYIRVTQALSRGELSLPSGSYGFGPGDNPIFPEMYET
                     EALKVGSALQAAELLYTGNCEVAFSYSGGLHHAGPDFASGFCVFNDCAVAIQWLVDQG
                     LRVAYIDIDVHHGDGVQNAFYDSDQVLTVSLHQDPRTLFPGTGFVEEIGRGAGTGFSV
                     NVPLPPLTFDAVYLRAFEAIVPPLLCRFSPDIVATQLGVDTHYTDPLANLALTTHGQL
                     ALFQALEALSPRWLAFGGGGYAIDLVPRSWALAFGVMSGQELPAELPPAYRKRYGGSL
                     LLDLEGPDITGPPQVRVSEAVDEVIAKVKQQHAL"
     gene            complement(1652..1724)
                     /locus_tag="VI520_00265"
     tRNA            complement(1652..1724)
                     /locus_tag="VI520_00265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1689..1691),aa:Arg,seq:cct)
     gene            complement(1789..2862)
                     /locus_tag="VI520_00270"
     CDS             complement(1789..2862)
                     /locus_tag="VI520_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF36345.1"
                     /translation="MDLSSAFGRPPRILVADDDWLNRDLLQAYLTTSGCEVVTAADGE
                     TALRLALNLPPDLALIDVQMPRLDGLEVCRRLKNTPATRFVPVVIITALDSEEEKLNA
                     FEVGADDFITKPYSSIVLLARVRSLLRIKRLHDEVESRNALLRQVLDRYVAEDVADVI
                     LTDPERHLKLFGETRPVTVLFADIRGFVRYTELHTGPEVIRTLNHVFQAVSKVVFAHK
                     GTFDKYLGDGLMAFYGAPVAGEDDAQRAVATAVEMQRLFAKLRENPEVDLKGLGLGIG
                     LHSGEAIVGNIGSEQVMDYTVVGDVVNVAKRLQDAAQSGQILFSQDTFEELKGVDAKK
                     LKPLKLPGRQERVTPYLIELAKR"
     gene            complement(2915..3616)
                     /locus_tag="VI520_00275"
     CDS             complement(2915..3616)
                     /locus_tag="VI520_00275"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HLF36346.1"
                     /translation="MQVELRIHRFNPEVDERAHFETYTVEAEPHDQVLDVLNQVKWYQ
                     DGSLAYRRSCGHGICGSDAMRINGVNRLACKVLVRDVGRSITVEPILGLPVVKDMIVD
                     MEPFWVHYRSVKPFFITKDPAPEQERLQSPEQRARFDDSTKCILCAACTTACPSFWAD
                     GEYVGPAAIVQAHRFIFDSRDAAADQRLEILGEQMGVWRCRTIFNCTVACPRDIKITQ
                     AIGEVKRAIATGKLD"
     gene            complement(3636..5429)
                     /locus_tag="VI520_00280"
     CDS             complement(3636..5429)
                     /locus_tag="VI520_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF36347.1"
                     /translation="MAELHQHDVLIVGGGGAGLMAALHASRSSKTAVLTKLHPVRSHT
                     GAAQGGICAALGSEEADRPEWHTFDTVKGSDYLGDQDAIEFMCEQAVPTIYELEHMGL
                     PFNRTSDGKIAQRYFGGHTNNETGKPVRRACYAADRTGHMILQTLYQQCIKHEVSFLD
                     EYQVMDILIDERGTAGVVAVELATGELHVFHSKAVIVSSGGWGRMYEVTSNAHSYTGD
                     GVALTLRRGIPAEDMEFFQFHPTGIYKMGILITEGVRGEGGVLINGRGERFMERYAPR
                     IKDLASRDVISRAMYLEMREGRGIDGGRYLHLDVRPDTVNRYAELDGRTRPDGSPYQV
                     TGEEILAKVPDIIDFCRTYLAVDPITQPMPVQPTAHYAMGGIPTNLRGEVVRDEANTV
                     VPGLYAAGEVACVSVHGSNRLGTNSLLDIVVFGRECGKRAAEYAAHADRLPLPADPEG
                     RVREQLDRLRSASGSEKVASVRKELQQVMFEQVGVFRSQDGMSEAVDKIGRMKDRYRE
                     VRLDDRGKVFNTDLLEAWELGCLLDIAEVTAVSALNRTESRGAHAREDYQERDDQRWL
                     KHSLAYLDGGKISLRYKPVTITKWQPQARTY"
     gene            complement(5481..>6408)
                     /locus_tag="VI520_00285"
     CDS             complement(5481..>6408)
                     /locus_tag="VI520_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36348.1"
                     /translation="PNGTILGLAGKAGLRMSTLSQTFQGALRYRGGEHQLSYFGHRIS
                     GLGTLLFLTIHILDTSTVYFFPNLYVHAINIYRSTPMMLGEMLLVVAVIAHGVNGLRI
                     AFSDFFPAWWQARPQRTAFWIVVAISTVLSIPALFFMGRSLYLNNICRCPPAEQASLA
                     LPVWADIAIGGSFVVALAVLARAGTFRTLFNRRPRNFETWMWLMMRWSAVLLIPLVWI
                     HVLLVDVLFGVHRIDLNYVELRWATLGWRVYDISLLAFAFAHGMNGLRGVMQDYVHSA
                     AWRRRFNWAIMGMWILWTAIGAVAIVGGVRAG"
BASE COUNT         1173 a         2115 c         1907 g         1213 t
ORIGIN      
        1 agctcgactt cggatccgag ctgcagcccg agcgggaaca ccgggttcga aaacacattg
       61 ctggggctga tcaaccagga gcgcgccgcg cagggcctgc cggcctatgc tgcacagggt
      121 cagctgcaga ccgcggcccg cctccacagc accgacatgg gctgcaacgg cttcttctcc
      181 cacaccggtt cggatggttc cacagtaggg ctgcgggtca ccgcccaggg ctacagctgg
      241 tcctgggttg gcgagaacat cttcgccggc accagcgcaa cgccgcagtc tgccttcgac
      301 tggtggatga acagcgcccc ccaccgggct aatctgctca gccccaacta caccgagatt
      361 gggctcggtt acatttatga ggccggcagc ccttacggcg ggtacttcac ggcagttttc
      421 gccaagccct cctcttaaca aacgacgaca ttcgcgcggc tacaatcacc ctccatgcgc
      481 cgagcggtct tcatggtctc accggagcta tgggagcgcg gtcacggacc gcagcatccg
      541 ctgaagcccg aacggctgca gcgcaccttc gagctgctca gcgaatatgg cgcgttcgcc
      601 gcgcctaacg tgcagattgt caagccgcgg ctggccgagc cggacgagct ggccctgttt
      661 cacacccagg actacatccg ggtgacccag gccctcagcc ggggagagct cagcttgccc
      721 tccgggagct acggcttcgg tccgggcgac aacccgatct tccccgagat gtacgaaact
      781 gaggctttga aggtcgggag cgccttgcag gccgccgagc tgctctacac cgggaattgc
      841 gaggttgcct tcagctacag cggcgggctg caccacgccg gtccggactt cgcctccgga
      901 ttctgcgtat tcaacgactg cgcggtcgca atccagtggc tggtagacca agggctgcgg
      961 gtggcctata tcgacatcga cgtccaccac ggcgacgggg tccagaacgc cttctatgac
     1021 agcgaccaag tgctcaccgt ctcgctgcac caggacccgc gcaccttgtt tcccggcacc
     1081 ggcttcgtcg aggagatcgg ccgaggtgcc ggcaccggct tcagcgtcaa tgttccccta
     1141 ccaccgctga ccttcgacgc ggtctaccta cgggcgttcg aggcgatcgt gccgccgctg
     1201 ctctgccgtt tctctcccga catcgtggcg actcaactgg gtgtggatac tcactacacg
     1261 gacccgctcg ctaacctggc cctcaccacc cacggtcagc tggcgctgtt ccaggcgctg
     1321 gaggcattgt ctccgcgctg gctggctttt ggcgggggtg gctacgccat tgatctggtg
     1381 ccgcgctcat gggcgttggc gttcggagtc atgtcggggc aggagctgcc ggcggagctg
     1441 ccgccggcct accgaaagag gtatggtggc agtctcttac tcgacctgga gggcccggac
     1501 atcaccgggc cgccccaggt gcgggtcagc gaggccgtcg atgaagtgat tgccaaggtg
     1561 aagcagcagc atgcgctctg aaggctgggg gaaccctatg cctccaagca aaaccgccgg
     1621 ggattcaagc ggccagccgg cggagtttgt atgccccagg cgcgaatcga acgcgcaacc
     1681 ttggccttag gagtgccctg ctctatcctt tgagctactg gggcccgttg ggattatatc
     1741 ccgccggctc aacctagcag tcggagtgcc gctgactcgc ttcgccgctc acctcttcgc
     1801 cagctcgatc agataaggcg tgacccgctc ctggcggcca ggcagcttga gtggtttcaa
     1861 cttcttggcg tccacccctt tcaattcctc aaaggtgtct tggctgaaca ggatctgccc
     1921 gctctgggcg gcatcctgca agcgcttggc cacgttgacc acgtctccaa cgaccgtgta
     1981 atccatcact tgctcggagc caatattgcc gacgatcgcc tcacccgaat gcaggccaat
     2041 tcccagtccc aggcccttca aatcgacctc tggattctcg cgcaacttgg caaagagccg
     2101 ctgcatctcg accgcagtgg ccaccgcccg ctgggcgtcg tcctcccccg ccaccggcgc
     2161 cccgtagaac gccatcagcc cgtcccctag gtatttgtcg aaggtgccct tgtgagcgaa
     2221 gaccactttg ctgaccgcct ggaacacgtg attcaaggtc cggatcactt ccgggccggt
     2281 gtgcaattcg gtgtaacgaa cgaaaccccg gatgtccgcg aataacacgg tcaccgggcg
     2341 ggtctcgccg aatagcttca ggtgccgttc cggatcggtc aggatgacat ccgccacgtc
     2401 ttccgcgaca tagcgatcca gcacctggcg gagcagtgcg ttgcgcgatt ccacctcgtc
     2461 gtgcagacgt ttgatccgca gcagcgagcg cacccgggcc agcagcacga tggagctgta
     2521 cggtttggtg atgaagtcgt cggcgccgac ttcgaaggca ttgagcttct cttcttcact
     2581 gtcgagggcg gtgatgatga ccaccggcac gaagcgggtc gcaggggtgt tcttcaacct
     2641 tcgacagacc tccagaccat ccagtcgcgg catctggacg tcgatcagcg ccaggtctgg
     2701 agggagattc agggcgagcc gcagtgcagt ctcgccgtcg gccgcggtta ctacctcaca
     2761 gccggaggtc gtcaggtagg cctggagcag gtcccggttg agccaatcat cgtccgccac
     2821 caagatgcgc ggcgggcgac caaaggccga cgagaggtcc atcgctgggt cctgcagcgt
     2881 ccgagcgttc ctcatcgtgc gggccgaccg agcttcaatc cagcttaccg gtggcgatgg
     2941 cgcgcttcac ctcaccaatc gcctgagtga ttttgatatc gcgggggcag gccaccgtgc
     3001 agttgaaaat cgtcctgcag cgccagaccc ccatttgctc gcccaagatt tcgagccgct
     3061 ggtcggcggc cgcgtcccgg ctgtcgaaaa tgaaacggtg ggcctggacg atggccgccg
     3121 gccctacgta ctcgccgtcc gcccagaagg agggacaagc cgtcgtgcag gccgcacaca
     3181 ggatgcactt ggtggagtcg tcgaagcggg cccgctgttc cgggctctgc aggcgctctt
     3241 gctcgggagc cgggtccttg gtgatgaaga aaggcttgac cgatcggtaa tgcacccaaa
     3301 atggttccat atccacgatc atgtctttca cgaccggcag cccgaggatc ggttccacag
     3361 tgatcgacct tccaacgtct cgcaccagca ctttgcatgc cagacgattc accccgttga
     3421 tccgcatggc gtctgatcca caaatcccat gaccgcagga ccgccggtag gccagacttc
     3481 cgtcttggta ccacttgacc tggttcaaca catccagcac ctggtcatgc ggctcggcct
     3541 cgaccgtgta ggtctcgaag tgggctcgct cgtccacttc cggattgaac cggtggatac
     3601 gcagctctac ctgcatgtac gaactctcat cgccctcaat acgtccgcgc ctgcggttgc
     3661 cacttcgtga tcgtgaccgg cttgtagcgg agtgagatct tgcccccgtc cagataggcc
     3721 aagctgtgtt tcagccagcg ctggtcgtcc cgctcttggt agtcttcccg ggcgtgggcg
     3781 ccccggctct cggtccggtt cagggcggag actgcggtga cctcggcgat atccagcagg
     3841 cagcccagct cccaggcctc cagcaaatcg gtattgaaga ctttacctcg gtcgtcgagc
     3901 cggacctccc gataacgatc cttcattcgc ccgatcttgt cgactgcctc gctcatccca
     3961 tcctgagaac ggaatacgcc tacctgctcg aacataacct gctgcagctc cttgcgcacg
     4021 ctggcgactt tctcggagcc gctggctgag cgcaaccggt ccaactgttc ccgcaccctg
     4081 ccctccggat cggccggcag cggcagccgg tcggcatgcg ccgcatattc cgcggcccgt
     4141 ttgccacact ctcggccgaa gaccacgata tccagcagcg aattggtgcc caaccgattg
     4201 gaaccgtgaa ccgaaacgca ggccacttct cccgccgcat acaggcccgg aaccaccgtg
     4261 ttggcctcat cccgaaccac ttcccctcgc aaattggtcg ggatcccgcc catggcgtag
     4321 tgggcggtcg gctggaccgg catgggctgg gtgatgggat ccaccgccag gtatgtgcgg
     4381 cagaagtcga tgatgtccgg cactttagcc aggatctcct cgccggttac ctgatagggt
     4441 gagccgtccg gccgggtccg gccgtcgagc tcggcatatc ggttaacggt gtccggccgt
     4501 acatccaagt gtagatagcg ccccccatcg attccccgtc cctcccgcat ctccagatac
     4561 atcgcccggc tgattacatc ccgagaggcc aggtccttga tccgcggcgc atatcgctcc
     4621 atgaatcttt cgccccggcc gttgatcagc acccctccct ccccgcgcac accttcggtg
     4681 atcaggattc ccattttgta aatgccggtc ggatggaatt gaaagaactc catgtcctcc
     4741 gccggaatgc cgcggcggag agtcagcgcg accccgtcgc cggtgtagga gtgcgcattg
     4801 gaggtgacct cgtacatcct tccccagcct ccgctgctta cgatcacggc tttcgagtga
     4861 aagacatgca gttccccggt ggcgagctcg actgccacca ccccggccgt gccacgctcg
     4921 tcgatcagga tgtccatcac ctggtactcg tccaggaagc tgacttcgtg cttgatgcat
     4981 tgctggtaaa gcgtttgcaa gatcatgtgg ccggtccgat cggctgcgta gcaggcccgg
     5041 cgcaccggct tgccggtctc gttgttggta tgcccgccaa aatagcgttg ggcgatcttg
     5101 ccgtcggagg tccggttgaa gggcagcccc atgtgctcca gctcataaat cgtgggcact
     5161 gcctgctcgc acatgaactc gatcgcgtcc tgatcgccca gatagtcgct tcccttgacg
     5221 gtatcgaagg tgtgccactc ggggcgatcc gcttcttcgc tgccgagcgc ggcacagatg
     5281 ccgccctggg cggcgccagt gtgcgaccgg accggatgta acttagtcag caccgcggtc
     5341 ttgctgctcc gggaggcgtg cagggcggcc atgagtccgg ccccgccccc acccacaatc
     5401 agcacgtcat gctgatggag ttcggccatt caactgcacc tctcttgctg aaacgctgca
     5461 aggttcctgc cggcggaggc tcagcccgct cggacgcctc cgacgatggc gaccgcgccg
     5521 atcgcggtcc agaggatcca catgcccatg attgcccaat tgaaccgacg acgccaggcc
     5581 gcagagtgga cgtagtcctg catgactccc cgcaagccat tcatcccatg ggcgaaggca
     5641 aatgccagca gcgagatgtc atacaccctc caaccgaggg tggcccagcg cagctcgacg
     5701 taattgagat cgatgcggtg caccccaaac aagacgtcca ccagcagcac gtggatccag
     5761 actagcggga tcaagagcac cgccgaccag cgcatcatga gccacatcca ggtctcgaaa
     5821 ttgcgcggcc ggcgattgaa cagcgtccgg aaggtcccgg ctcgggccag gaccgccaat
     5881 gcgaccacga acgatccgcc aatggcgatg tcggcccaga ccggtagagc caggctggcc
     5941 tgctcagccg gcggacatcg gcagatattg ttcaagtaaa gcgaacggcc catgaagaag
     6001 agcgccggta tgctgagaac ggtgctaatt gccaccacga tccagaaggc ggttcgctgc
     6061 ggccgcgcct gccaccaggc cgggaagaag tccgagaagg cgatccgcaa cccgttgacg
     6121 ccatgggcga tcactgccac gaccagcagc atttccccca gcatcattgg ggtggagcgg
     6181 tagatgttga tggcgtgtac gtataagttg gggaagaagt agaccgtgga ggtatcgagg
     6241 atgtgaatcg tgaggaaaag cagcgtgccc agcccgctaa ttctgtgtcc gaagtaggat
     6301 agctggtgtt cccctcctcg atagcgcagc gcgccctgga aggtttggct cagcgtcgac
     6361 atgcgcagtc ccgccttccc cgcgagcccg aggattgtac cattgggg
//
LOCUS       DATDPQ010000014         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_266487, whole genome shotgun sequence.
ACCESSION   DATDPQ010000014 DATDPQ010000000
VERSION     DATDPQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266487"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VI520_00290"
     CDS             complement(<1..1120)
                     /locus_tag="VI520_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLF36349.1"
                     /translation="MPNAIAATAAAGMEFLPLLFLLAALIASAKIFGSLSLRLGQPAV
                     LGELVAGLILGPSLLHLASLPFLRSGDPGATIHLIGELGVILLMFAAGLEVDIDDLRR
                     SGRPAALAGILGVMVPLGGGAAVSLAFGHEAPAAIFVGIVLGATSVSISAQTLLELGR
                     LRSREGVTMLGAAVVDDVLVIAVMTVILATTGGEAGSFGAVGLTLLQMVIVLSLVGFV
                     SLRLLPRLVEWAHRLRVSQGLLSFSLAAVLLLAWFTEFAGGVAAITGAFLAGLGLSRS
                     HLRDEIEHGVSRLAYGFFVPIFLVDIGLRSNLREMQADQWGFAAVIVGVAILSKPIGA
                     GLGSWLGGFDPGSAIRLGLGMISRGEVGLIVAGIGIGEG"
     gene            complement(1266..>2103)
                     /locus_tag="VI520_00295"
     CDS             complement(1266..>2103)
                     /locus_tag="VI520_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36350.1"
                     /translation="FSELLNGRRLVAGSAPWDGDRASLRAVDALLGVDGSPQSATGQA
                     ALLTGRNVPALVGEHYGPKPNQAVAGIVRADNLFKQVVQRGGSAALLNGYPPRYFQSI
                     ESGHRLYSSIPLAATAAGLELKSVEDMQAGRAMAADFTGAGWAAQPGFPAAPIYSPEQ
                     AGHQLAVLAQRYDLAWFDYWVSDLVGHRGELADAVALLENLDGVIGGLARAWGDRGDL
                     IVISSDHGNLEDLSRRGHTRNPVPALLIGGPEERLAFAADLRDLTSFAPAILALIFGG
                     RG"
BASE COUNT          438 a          729 c          624 g          312 t
ORIGIN      
        1 aaccttcgcc gattccaatc ccggccacaa tcaggccgac ctctccgcgt gaaatcatcc
       61 ccaaccctag ccggattgcc gatccgggat caaatccacc gagccacgat cctaggccgg
      121 caccaatcgg ctttgacaag atcgcaacgc cgacgatcac cgcggcaaat ccccactgat
      181 cggcctgcat ctcccgaagg ttggatctca atccgatgtc caccaggaat ataggcacga
      241 agaatccgta ggcgaggcgc gagactccat gctcgatctc gtccctgagg tgacttcggc
      301 ttaggccgag ccccgccagg aaagcacctg taatcgcagc cacgccaccg gcaaactcgg
      361 tgaaccaggc gagcagcagc actgcggcca aggagaagga gagcagcccc tggctgaccc
      421 gcagccggtg ggcccactcg acgagccttg gcagaagccg caaagagaca aaccccacca
      481 ggctgagcac gataaccatc tggaggagag tcaggccgac cgcgccgaag ctgcccgcct
      541 cgccgccggt agtggcgaga atgacagtca tgactgcaat taccagcacg tcgtccacaa
      601 ccgccgcccc cagcatggtg acgccctctc gtgatcgcaa cctgcccaac tcgagcaatg
      661 tctgggcgga gatgctcacg ctggtggccc ctaaaacaat cccgacgaag atcgcggccg
      721 gggcctcgtg gccgaaggct agcgagaccg ccgcccctcc gcccaatgga accatcacac
      781 ccaggattcc ggccagcgcg gccggcctac cggatcgccg cagatcatcg atgtccacct
      841 cgaggccagc cgcaaacatc agcaagatga cgcccagctc cccaatgagg tggattgttg
      901 cacctggatc gccagagcga aggaagggga gggatgcgag atggagcagg ctcggcccga
      961 ggatcaggcc cgcgaccagc tcacccagaa ctgcaggttg gccgaggcgg agggataggc
     1021 tcccaaagat cttggcggag gcgattagcg ctgccagcaa gaacaggagc ggcaggaact
     1081 ccataccagc cgcagctgta gcggcaatcg cattgggcat cctcagactc cggaggctcc
     1141 atccacatac gatagcttga gagggtcggg cgcggcccga ccgaccggat tatagcatcg
     1201 gtctcccgcg gcccttcagc tggctcagcc cctggggccc tggtccaagt cgggcatctc
     1261 ggtgctcacc cccggcctcc gaagatcaac gccaggatag cgggggcgaa tgaggtcaga
     1321 tcgcgcaggt cggccgcaaa agcgagtcgt tcctccggcc caccgatcag gagtgccggc
     1381 accgggttcc gggtgtgacc gcgccgcgag agatcttcca gattaccgtg gtccgaactg
     1441 atcacgatta ggtctccccg atcgccccag gcgcgggcca accctccgat gactccgtcc
     1501 aggttttcca gcagggcgac tgcgtcggcc agctcgcccc ggtgcccgac cagatcgctc
     1561 acccaatagt cgaaccaagc cagatcgtat cgctgcgcca ggaccgccaa ctgatggccg
     1621 gcttgctccg ggctgtagat cggcgcggct ggaaagcctg gctgagccgc ccagcccgcg
     1681 ccggtgaaat cggccgccat ggcccttccg gcctgcatgt cctcgacgct ttttaactca
     1741 aggcccgccg cggtggcggc caatggaatc gaagaataca atcgatggcc gctctcgata
     1801 ctctgaaaat atcgcggcgg atagccattc agcaacgcgg ccgacccccc tcgctgcaca
     1861 acctgcttga ataggttgtc ggcccgcacg atccccgcaa ccgcttggtt gggtttcggg
     1921 ccatagtgtt caccaaccag ggccggcaca ttgcgtccgg tcagcagcgc ggcctgtccg
     1981 gttgcggact ggggcgatcc gtcgactccc agcagcgcat cgaccgcccg caggctggcc
     2041 cggtcgccgt cccagggggc cgaaccggcg accagccgcc tgccgttcaa cagctcagaa
     2101 aag
//
LOCUS       DATDPQ010000015         7481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3405143, whole genome shotgun sequence.
ACCESSION   DATDPQ010000015 DATDPQ010000000
VERSION     DATDPQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405143"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VI520_00300"
     CDS             <1..1707
                     /locus_tag="VI520_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF36351.1"
                     /translation="TLRDPDNLVYFREEVGGLVAGGYERAPAPWGLDGIPPDFNNKLL
                     PPDWERFSPLMENAIRRVPALERAEVITLINGPEGFTPDAEYLLGPTGVKGFWVAAAF
                     CAHGLAGAGGLGKAMAEWIIDGVPEWDIWRLDVRRFGSNYTGQQYVVARTVETYSKYY
                     DIHYPNEERESARNLRLSPAFQRLRELGASFGEKTGWERPNWFEPYEARANHGHEPRG
                     WARHHWSPAIGVEHLTTRERAGLFDETSFSKLDVRGQGSVDLLQRLCANDIDRPVGTV
                     VYTSMLNPRGGIECDFTVTRLAPDHFRIVTGTAFGQHDHSWIRMNQQANGSVAIEDVT
                     GALACFGLWGPRARLILQRVTKQDISADAFPYMTARQITVGDVPCLALRVTYVGELGW
                     EFYCSMEYGQRLWDVLWEAGQPDGLAAAGYRAIDTLRLEKGYRYWSADIGPDYTPYEA
                     GLGFAVSLSKPEFIGRAALVRQKEQGLRRRLRCLTLADPAAIAIGNEPIRTPDDSQVV
                     GWVTSGGYGYSVGKSIAYGYLPTAHAAFGTRLDIEILGERIAAEVAREPLWDPKGERI
                     KA"
     gene            1715..2626
                     /locus_tag="VI520_00305"
     CDS             1715..2626
                     /locus_tag="VI520_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HLF36352.1"
                     /translation="MPLTLAEAIQRVPFLATANDLRTTPLAGGITNLNYRIDAGDRSY
                     VLRITGAGTEMLGIRREIEHAANLAAGRLGVSPEVVFFIEPEGYLVTRFVDGVPIPPE
                     QMGKPENLRRVAEKIRAFHEGAPPLAGEFNVFRRVEMLTEVSRRHNCRFPDNFDWIMQ
                     RMRQVEHSLAVSAAILHPCHDDLLNLNFLDEQGEIRILDWEYAGMGEVTYDLANFSSH
                     HRLNDDQLRLLLQAYFGEATEQQFARLKLLWPMSEIHEAMWGTTQTGISKLDEDFQAY
                     ADLWFGRATEAMSDTRWDQWIRQAVMR"
     gene            2599..3282
                     /locus_tag="VI520_00310"
     CDS             2599..3282
                     /locus_tag="VI520_00310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLF36353.1"
                     /translation="MDSPGGYAVIAQPKLSRGETLISTIRHGLTELNRDKRVGGHTDV
                     PLLEVGHQQAEAAHATFEGTPFDLVISSPLRRAIETAVIVTGIARQELIIEPLCIERF
                     FGEMEGLTRPQVEERFPQIVYLQIGHIGNSLNPPGGESYEALRRRAEHFLEKVLAEHS
                     GRRIVISSHQAFMQQLHGLLLGRDPYDSLRADLLNLELNQFHLGPQRNLLQHQKVHLI
                     PDAQKYASF"
     gene            3269..4570
                     /locus_tag="VI520_00315"
     CDS             3269..4570
                     /locus_tag="VI520_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HLF36354.1"
                     /translation="MRPSDPLLAAISEQYVSHFREALGSIRKVGREAEFPVVWPDGRA
                     GDVNLLWEPLLAQGGLTPHYDDPGARHLLVEVSEPGMMVAIEVGRCTVELSLGPYEDL
                     WQLQAGFDRALARVVGAARERGMLLLGFGIQPRTPASQRLMTPARHYRALCRAAGTAW
                     LRLTTSASDQLHVDITRSEIPAAVNWMNLLSGPLIALCANSSVYAGRAGRYQAGREAF
                     LADLGEQRYGMPPRQFDTTTEWIQYLSEFRCFVLPESTGFRRTNRPFSEVLTAEAKRG
                     TSAEQLFQYFLWHEHYVWNSARARVAHSTIEVRPACQQPPGESLAANALILGWVVALK
                     EASTLISRTLGPDPWPAMSRYRQAAVREGLGAPEPAPGLLTGLVEIARGGLKRRGRGE
                     ERLLVPIEQRLAKRKSPGQRAQQILRRSGMAALLEQLRLTA"
     gene            4632..5504
                     /locus_tag="VI520_00320"
     CDS             4632..5504
                     /locus_tag="VI520_00320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HLF36355.1"
                     /translation="MTLDIPLHLCIINGYPRANRKVLAEAGVSQADELYLNFLRRYAP
                     KGRFDILYVADLDVGLPSGTGLASYDAYIWTGSNLTIYHDRPEVSRQIELCRSIFEAG
                     VPQYGSCWGVQMAAVAQGGEVRKNPNGREWCIARDITLTEAGRAHPLYAGKPDRFNGF
                     IMHLDEVSRAPQGGTVLASNRHTPVQSLAVRHASGGEFWATQYHPEYNLYEMARLLVA
                     RKKPLTEEGFFNEEADVERYVDKLTALWNQPEDPALRSELNIGSDILGAEIREAELRN
                     WLHYLVIPQMQPAA"
     gene            5505..6278
                     /locus_tag="VI520_00325"
     CDS             5505..6278
                     /locus_tag="VI520_00325"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLF36356.1"
                     /translation="MRLQDKVALITGAGSGIGRETALLFAAEGAAIVVVDVNDSAGAE
                     TVRLVHEQGGQATYAHADVSKAADCESMVRRAEESYGKLNVLFNNAGIMHSADDDAIT
                     TTEEVWDLTMAINLKGVFFGCKFGIPALRRAGGGSVINTASFVALIGAATPQVAYTAS
                     KGGVLALTRELAVVHARENIRVNALCPGPLKTPLLMNFLNTEEKKQRRLVHIPMGRFG
                     EAKEMAQAALYLASDESSFLTGATFSVDGGITAAYVTPL"
     gene            6289..>7481
                     /locus_tag="VI520_00330"
     CDS             6289..>7481
                     /locus_tag="VI520_00330"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF36357.1"
                     /translation="MEPIPFQGLAVDGKRVAAADGREVEVFDPANGEVLARVAQAGEP
                     DVERAVLAARQRYEDRAWRTMNTRKRGRILQRIADLVRQEKESLARLESRNAGKPIKA
                     ARGEIAAVANCFEFYAGATNKFYGETIPVSAHGTSLTFHEPLGVCALIVPWNYPLLIT
                     SWKVAPALAMGNTVIIKPASPTPLSALALADLALEAGLPPGALNVLPGSGSTVGSALV
                     RHPAVRKVSFTGSTEIGAGIMKMAADDIKRVSLELGGKSANVVFADADLSVCVESSIF
                     SVYDNAGQDCCARSRILVERSIHDEFVERFVARAKTLRLGDTQDEQTEVGPLITPQQR
                     ESVRHYLEVGDGEGAERLCGGEVPGDGPLARGNFLTPAVYVEVRPDMRIAQEEIFGPV
                     VAIIP"
BASE COUNT         1354 a         2365 c         2420 g         1342 t
ORIGIN      
        1 accctgcgcg acccggacaa cctggtgtac tttcgggagg aggtcggagg actggtcgcg
       61 ggcggctacg agcgggcgcc ggctccctgg ggcctggacg gcatcccgcc cgacttcaac
      121 aacaagctgc ttccacccga ctgggagcga ttttccccgc tcatggagaa cgccatccgg
      181 cgggtgccgg ccttggagcg agcggaggtc atcactctga tcaacggccc agagggcttc
      241 acccccgatg cagagtacct gctcggcccg accggtgtga aaggcttctg ggttgcggcc
      301 gcgttctgcg cccacgggct cgcaggagcc ggaggcctcg gcaaggccat ggcggaatgg
      361 atcatcgacg gcgtaccgga gtgggatatc tggcggttgg acgtccggcg cttcgggtcc
      421 aattacaccg gccagcagta cgtcgtggcg cggacggtgg agacctattc caagtactac
      481 gacattcact acccgaacga ggagcgagag tcggcccgca acctgcgcct atcacccgcc
      541 tttcagcggc tacgcgagct tggggcttcg tttggagaga agaccggctg ggaacgccca
      601 aattggtttg aaccatacga ggcccgtgct aaccacggtc atgagccgcg gggatgggct
      661 cgccaccatt ggtcaccggc gatcggggtc gaacacctga ccactcgcga gcgggccggc
      721 ctgttcgacg agacgtcgtt cagcaagctc gatgtccgag ggcaagggtc ggtcgatctg
      781 ctccagcggc tgtgtgccaa cgacatcgac cgcccggtgg ggacggtggt ctatacctcg
      841 atgctcaatc cgcggggtgg gatcgagtgc gacttcaccg tgactcggct ggcgcccgac
      901 cacttccgaa tcgtcaccgg gaccgctttc gggcagcacg atcactcctg gatccgcatg
      961 aatcagcagg caaatggatc cgtagcgatc gaggacgtca ccggcgcttt ggcctgtttc
     1021 ggcctgtggg gtccacgggc ccggctcatc ctgcagcggg tgaccaagca ggacatttcg
     1081 gcggatgctt ttccttatat gacggcccgg cagatcacgg tgggcgatgt tccatgcctc
     1141 gccctccggg ttacctacgt cggagagctg ggatgggagt tctactgctc gatggaatac
     1201 ggccagcggc tctgggacgt gttgtgggaa gccggccaac cggacggcct ggcggccgcc
     1261 ggctaccggg cgatcgatac cctgcggctg gagaaaggct atcgctactg gagcgccgac
     1321 atcggtcccg attacacgcc ctatgaggcg ggcctcggtt tcgcggtcag cctgagcaag
     1381 ccggaattca tcggacgggc ggcactcgtc cgtcagaagg agcaaggcct gcgccgtcgg
     1441 ctgcgctgcc tgaccctggc cgatccggcg gcaattgcga tcggcaacga gcccatccgg
     1501 acccccgacg attcgcaagt cgtcggctgg gtcacatctg gcggatacgg ttattcggtc
     1561 ggcaagagca tcgcctacgg ctatctgccc accgcgcacg cggcgtttgg cacccgcctc
     1621 gacattgaga ttttgggcga gcggatcgcg gccgaggtgg cccgagagcc gctttgggat
     1681 ccgaaaggcg agcggatcaa ggcctgaagc gccgatgccc ttgacgcttg cggaggcaat
     1741 tcaacgggtc cccttcctgg ccaccgccaa cgatctgcgc accacgccac tcgcgggagg
     1801 cattaccaac ctgaactacc ggatcgacgc cggcgatcgt tcgtatgtcc tccgaattac
     1861 cggcgccggg acggaaatgc tcgggatccg gcgggagatc gagcacgcgg ccaatctggc
     1921 cgccggccgg ctcggggtct cgcccgaagt ggtgttcttc atcgaaccgg aagggtacct
     1981 ggtgacccgt tttgtcgacg gggtcccgat cccgcccgag cagatgggaa agcccgagaa
     2041 cttgcggcgg gtcgcggaga agatccgcgc gttccatgaa ggtgccccgc cgctcgccgg
     2101 ggaatttaat gtgtttcgcc gggtggagat gctgaccgag gtctctcgcc gtcacaactg
     2161 ccgctttcca gacaacttcg actggatcat gcagcggatg cgccaggtcg agcacagcct
     2221 ggccgtgagc gccgcgattc tccatccgtg tcacgacgat ctgctgaatc tcaactttct
     2281 agatgagcaa ggagaaatcc ggatcctcga ttgggagtat gccggcatgg gcgaagtcac
     2341 ctacgacctc gccaatttct cttcgcacca tcgtctgaac gatgaccagc tgcggctgct
     2401 gctgcaggcc tacttcggcg aggccaccga gcagcagttc gcccgcctga agctgctgtg
     2461 gccgatgtcg gagatccatg aggcgatgtg gggcaccacc cagaccggga tatccaaact
     2521 cgacgaggat ttccaggcct acgccgatct gtggttcggg cgggcaaccg aggcgatgag
     2581 cgacacacgc tgggatcagt ggattcgcca ggcggttatg cggtgatcgc ccagcctaaa
     2641 ctctcgcgtg gggagacgct gatctcaaca atccgtcacg gattgactga gctcaaccgg
     2701 gacaagcggg tcggcgggca taccgacgtt ccactgctgg aggtcggcca tcagcaggcg
     2761 gaagcagcac acgcgacctt tgaagggaca cccttcgacc tcgtcatcag ttcccctctc
     2821 cggcgggcga tcgagaccgc ggtcatcgtc acgggtatcg ccaggcaaga attgattatc
     2881 gagccgttgt gcatcgaacg attctttggg gagatggagg ggctgacccg gccgcaggtg
     2941 gaagagcgct tcccccaaat tgtctatctt cagattggcc atatcgggaa ctctttgaat
     3001 cctccgggcg gtgagtctta tgaagcgctg cgccgtcggg cggagcattt cttggagaaa
     3061 gtcctcgcgg aacattccgg ccgcaggatc gtgatctcct cccatcaggc tttcatgcag
     3121 cagctgcacg gcctgctatt ggggcgggat ccatatgact cgctgcgggc ggacctgctc
     3181 aacctggagc tcaatcagtt ccatctcggt ccgcagcgaa acctcctcca gcatcagaag
     3241 gtccatctga ttccggacgc tcagaagtat gcgtccttct gacccgctgc tcgccgcgat
     3301 ctccgaacag tacgtgtcac attttcgaga ggccctgggg tccattcgca aggtcggacg
     3361 cgaggcggaa ttcccagtgg tatggcccga cggccgggcc ggggatgtca atctgctctg
     3421 ggaaccgctg ctggcccagg gcggtctcac cccgcactac gacgacccgg gcgcccggca
     3481 cctgctggtt gaagtgagcg agccgggaat gatggtagcc atcgaggtcg gccgctgtac
     3541 ggtcgagctg tcgctgggac cctacgaaga tctgtggcag ctgcaggctg gcttcgaccg
     3601 ggcgctggct cgggtggtgg gggccgcccg cgaacgcggc atgctgttgc tggggttcgg
     3661 gatccagcct cgcactccgg cctcccagcg attgatgact cccgcccgtc actaccgggc
     3721 gctttgtcga gcggctggga cagcctggct tcgcctgacg acctctgcct ccgaccaact
     3781 gcacgtggac atcactcggt ccgagattcc cgccgcggtc aactggatga acctgctctc
     3841 cggcccgctc atcgcgctgt gcgccaactc gtcggtctac gcagggcgag ccggccgata
     3901 tcaggccggc cgggaagcat ttctggcgga cctgggggag cagcgctacg ggatgccgcc
     3961 gcgccagttc gacaccacga ccgagtggat ccagtacctg tccgaatttc gatgtttcgt
     4021 gttgccggag tcaaccgggt ttcgccgaac taaccggccc ttcagcgagg tgctgactgc
     4081 cgaggccaag cgcggcacat ccgccgagca gctcttccaa tactttctct ggcacgagca
     4141 ctacgtttgg aattcggccc gggcccgggt ggcccattcg acgatcgagg tccgcccggc
     4201 ctgccagcag ccgccgggtg agtcgttggc tgccaatgcg cttattctcg ggtgggtcgt
     4261 ggctctgaaa gaggctagta cgctgatttc gaggactctt ggtcccgacc cctggccggc
     4321 gatgagccgc taccgccagg ccgcggtgcg agaggggctg ggcgctcccg agcccgctcc
     4381 gggcctgctg accggcctgg tcgagatcgc ccggggcggg ctgaagcgcc gcggccgagg
     4441 cgaagaacgt ctgcttgttc caatcgagca acgcctggcc aaacgcaaat cgccgggcca
     4501 gcgcgcccag caaatcctgc gtcgttccgg gatggcagcc ctgctcgagc aacttcgtct
     4561 cacagcgtga gcgccccgcg cccagcctcc aaaatcatga ggatataatc ccggcgcggg
     4621 agactgacac catgaccctc gacatcccac ttcacctgtg catcatcaac gggtacccac
     4681 gcgccaaccg caaggtcctg gcggaggcag gagtatccca agccgatgag ctttatttga
     4741 atttcctgcg gcgctacgct cccaaaggcc gcttcgacat cctgtacgtg gccgatctgg
     4801 acgtcggatt gccatccggg acggggctcg cttcctatga tgcctacatc tggaccggct
     4861 ccaacctgac catctatcac gatcgccccg aagtctcccg gcagatcgag ctgtgccggt
     4921 cgatcttcga ggccggcgta cctcaatacg gcagctgttg gggggtccag atggcagccg
     4981 tggcgcaagg cggtgaggtc aggaagaatc ccaatgggcg cgagtggtgc atcgcacgcg
     5041 acatcacact gaccgaggct ggccgggcgc atccgctcta tgccggcaag cccgatcgat
     5101 tcaacgggtt catcatgcac ctggacgagg tcagccgggc gccgcagggc ggcacggtgc
     5161 tggccagcaa ccggcacaca ccggtccaat cgctggcggt gcgccacgcc tcagggggcg
     5221 agttttgggc cactcaatac caccccgagt acaacctgta cgagatggcc cgcttgctag
     5281 tcgctcgcaa aaagccgctc accgaagaag gcttctttaa tgaagaggcc gacgtggagc
     5341 ggtacgtcga caagctcact gccctttgga accaacccga agatccggcg ctgcgaagcg
     5401 aactcaacat cggctccgac atccttgggg cagagatccg ggaggccgaa ctgcgcaact
     5461 ggcttcacta cctggtcatt ccccagatgc agccggcggc ctgaatgagg ctgcaagaca
     5521 aagtcgcgct gatcaccggc gccggcagcg gcatcggccg tgagacggca ctgctctttg
     5581 cggcggaagg cgcggcgatc gtcgtcgtgg acgtcaacga ctcggcagga gcggagactg
     5641 tccggttggt gcacgagcag gggggccagg cgacctacgc ccatgccgac gtctcaaagg
     5701 ctgctgactg tgagagcatg gtacggcgcg cggaagagag ctacggcaag ctgaatgtcc
     5761 tgttcaacaa cgccgggatc atgcactccg ccgatgacga cgccatcaca acaacggagg
     5821 aggtctggga cctgacgatg gccatcaacc tcaagggcgt cttcttcggc tgcaagttcg
     5881 gcatcccggc ccttcgtcgc gcggggggcg gctcggtgat caacaccgca tcctttgtcg
     5941 ccctcattgg cgccgccacc ccgcaggttg cctatacggc cagtaagggc ggcgtgctgg
     6001 ccctcacccg cgagctagct gtggtacatg cccgggaaaa tattcgggtc aatgccttgt
     6061 gccccggtcc gctgaagact cccctgttga tgaacttcct caacaccgaa gagaagaagc
     6121 aacgtcggct cgtgcatatt ccgatgggtc gcttcggaga ggccaaggag atggctcagg
     6181 ccgcgctcta cttggcctcg gacgaatcat cattcctgac cggggcgacc ttctcagtag
     6241 atggcggcat caccgctgcc tacgtcactc cgctctagga gagctgcaat ggagcccatt
     6301 cccttccaag gcctggctgt agatggcaaa cgggtggccg ccgcggatgg acgcgaggtc
     6361 gaagttttcg atccggccaa cggagaggtg ctggctcggg tggcgcaagc cggcgagccc
     6421 gatgtcgagc gggccgtgct ggccgccagg cagcgctacg aggatcgagc ctggcggacc
     6481 atgaacaccc gcaagcgcgg gcggatcctc cagcgaattg ccgatttagt gcgtcaggaa
     6541 aaagaaagcc tggcccggct ggagtcgcgc aacgccggca aaccgatcaa ggccgcccga
     6601 ggggagatcg cggcggtcgc caactgtttt gagttttacg ccggagcgac gaacaagttc
     6661 tacggggaga ccatcccggt atccgcccac ggcacttccc tgacgtttca cgaaccgttg
     6721 ggggtctgcg cattgatcgt gccctggaat tacccactgc tcatcaccag ttggaaggtg
     6781 gcgcccgccc tggcgatggg aaacaccgtg attatcaagc cggccagccc cacgccgctc
     6841 agtgccctgg ccctggccga cctggcgttg gaggccggcc tgcccccggg ggccttgaac
     6901 gtgctgccgg ggtcgggctc aacggtcggt tcggccctcg tccgtcatcc ggcggtccgc
     6961 aaggtctcgt ttaccggttc caccgagatc ggggccggga tcatgaaaat ggccgccgat
     7021 gacatcaagc gggtgtcgtt agagctgggt ggcaaatcgg ccaacgtcgt gtttgccgac
     7081 gccgatctat cggtgtgcgt cgagtcatcc atcttttcgg tctatgacaa cgccggccag
     7141 gattgctgcg cccgcagccg gatcctggtg gagcggtcga tccatgatga attcgtcgag
     7201 cggttcgtag cccgtgccaa gaccctccgg cttggcgaca cccaggatga gcagactgaa
     7261 gtggggccgc tgatcacgcc ccaacagcgt gaatcggtgc ggcactacct ggaggtcggc
     7321 gacggcgagg gagcggagcg gctgtgcggt ggcgaggtcc ccggagatgg cccgctggcg
     7381 cgaggcaact tcctgacccc ggcggtctac gtcgaagtca ggccggacat gcgcatcgct
     7441 caggaagaga tcttcggccc ggtggtggcg atcatcccct t
//
LOCUS       DATDPQ010000016         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2362103, whole genome shotgun sequence.
ACCESSION   DATDPQ010000016 DATDPQ010000000
VERSION     DATDPQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362103"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            954..1433
                     /locus_tag="VI520_00335"
     CDS             954..1433
                     /locus_tag="VI520_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36358.1"
                     /translation="MRHKRIVILLVVALSLAAAGTYAASDWLKGSTDEKLKTLAEIQP
                     GLGTVMIEYGARFTNLYYAARGGNWDLAAYQLKEAREIQEVGETSRPARAEALKGFEK
                     SYLDPIDAAIKAKDFKKFDKAFMDGIQGCNTCHMGQGFPFIKYQLPKGPTFPLSMKP"
     gene            complement(1661..2080)
                     /locus_tag="VI520_00340"
     CDS             complement(1661..2080)
                     /locus_tag="VI520_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36359.1"
                     /translation="MDSIIEYRNDQLATNDYPRRIVSPMTPSACCMQHMERIGRPAVD
                     AAWRFYYKRCAVCGYTVRCFYAPSILAILEAAKQIKLTLAEMNLGAGKRKRRTQAEIE
                     AEIAAAGYPLGGPLPTLKRARRSLMPLRPRKPIPSPA"
     gene            complement(2134..>2361)
                     /locus_tag="VI520_00345"
     CDS             complement(2134..>2361)
                     /locus_tag="VI520_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLF36360.1"
                     /translation="GLTPGEGALVAAVQPGSAADRAGLRPGDVIQEVNRKKVTSVKEA
                     QAESQKDPDARSLLVLVRRDGTSLFAALERK"
BASE COUNT          425 a          739 c          749 g          448 t
ORIGIN      
        1 tcaggattct gcagagcgtc gtcttgccgg ccccattcgg accgacgaga ccgatccgct
       61 gcccgcgcgt gatgtgccat gtcaggtcct tgaagacgac ctgcccgcca aaggacttgc
      121 cgatgttctc gagctggatc atagggatcg tcctgcattc agtcggccgg actctggggg
      181 cttggcgacc tcactctggg ccacaagaga aagagaccat actacgagaa tggggcctgg
      241 gcaagttgcc aactcgggct gactgggagt aggatccggc gggaggaaag ggcattcagc
      301 ccagacaacg caactctcgg cacttgccgg caaagtggcg cagtgctatt ttttaatcac
      361 cggcgaggct ggaatttctc cctctggact gcctgcatga gcgatcgtca gaagggctac
      421 caggcggcga ggcggcagca tcattgcggg cgcactataa ggcaaggaca tctttcgatc
      481 catgaagccg cttgaaggaa acctccgaag tttgaagggc tggaggaatg ccgtggccct
      541 tctcaccgcg atccttgccg cggttgtcca atgcggtgtt ccagccctcc tggcaggcgc
      601 cgaggttccg gaactcctgg atgccccatc tctcgaattc tggtcccttc ccccaaaccc
      661 gccaaggttg tctcctgttg gtcctgcgcg cttctgcgtc ggaggtctgc tctgcaacgc
      721 cgcggtgcgc tcgctgacat ccgacggccc tgttcgccca cgacctcctc ctgtcgcctg
      781 acatatggcg cccggccgcc ccgagatttg gggcggcatc caagggagac tcagcgagaa
      841 tcactggcca acgggttctt ctggttagtg ccagagggtg cctcggtgtt ggccgtcttt
      901 ttcctgtggg cgtttagtgg ccgagcgggt atcggcctcg ggaggactac atcatgcggc
      961 ataagcgcat cgttatcctg ctggtcgtgg ccctctccct ggcagcagcc gggacctacg
     1021 ctgcctcgga ttggctcaag ggcagcaccg atgagaagct taagaccctc gccgagattc
     1081 aacctgggct cgggacggtg atgatcgagt acggtgcccg cttcacaaac ctgtactacg
     1141 ccgccagggg gggaaactgg gatctggccg cctatcagtt gaaggaggca cgcgagatcc
     1201 aggaagtcgg tgagacgagc cgtccggcgc gggccgaggc cctgaaggga ttcgagaaga
     1261 gctacctgga cccgatcgat gcagccatca aggcaaagga cttcaagaag ttcgacaagg
     1321 cgttcatgga cgggatccag ggctgcaaca cttgccacat ggggcagggg ttccccttca
     1381 tcaaatacca actcccgaag ggacccacgt tcccgctctc gatgaagccc tgacaatctc
     1441 gcgcgtggta agagcaaacg ggtaggtcca gtcggacctg cccgtttgct tttcggagac
     1501 ggcgaggcat tgcaacgcgc gcacctcttc gggaaggagg tctctttcgg ctgagaaagg
     1561 tcaagacagg ggtggaaacg caggataagc ccctgtaggc tggcattccg gaccgccggc
     1621 cttcgtgggc ggccgatggc gagaagctcg gttccccgag ttaggcgggc gaggggatcg
     1681 gtttgcgggg ccggaggggc atcagagacc ggcgggcgcg cttcagggtg ggcagcggcc
     1741 caccgagcgg atacccggcg gcggcgatct ccgcctcgat ctccgcctgt gtccgccgct
     1801 tccgtttgcc ggcacccagg ttcatctcgg ccagcgtgag cttgatctgc ttcgccgcct
     1861 ccaggatagc caggatgctg ggcgcgtaga agcaccgaac ggtgtagcca cacaccgcgc
     1921 accgcttgta atagaagcgc caggccgcgt caacggccgg gcgaccgatc cgctccatat
     1981 gctgcatgca gcaggccgac ggcgtcatcg gcgacacgat ccgccgcggg tagtcattag
     2041 tcgctaattg gtcattccga tactcgatga tgctgtccat cgtgactccc ggtgggtggg
     2101 gctggcccta cccccatttc aactccttcc gacctatttc cgttccagcg ctgcgaacag
     2161 gctggtcccg tcccgccgga ccaggaccaa cagcgatcga gcatccgggt ccttctggga
     2221 ctcggcttgc gcctccttga ccgaggtgac cttctttcgg ttcacctcct ggatgacatc
     2281 gccgggccgg aggccggccc gatcggctgc gctgcccggc tggacggccg ccaccagcgc
     2341 cccctcgccg ggagtgagcc c
//
LOCUS       DATDPQ010000017         9339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1576722, whole genome shotgun sequence.
ACCESSION   DATDPQ010000017 DATDPQ010000000
VERSION     DATDPQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576722"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VI520_00350"
     CDS             <1..442
                     /locus_tag="VI520_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36361.1"
                     /translation="GEQTLFTWPYDPEFVPGTISWDGVLPDGKSAAPGEYGVYATAWD
                     LAGNQGQDAGVIRVPGSDLLAPIGELLANTPLRGVGQPDQDPQGASLSRPSSRISSSL
                     WLWPTIGWIGLLGGVTVSKMLDPRPAALNRLRQELAQVRAWQAE"
     gene            455..1018
                     /locus_tag="VI520_00355"
     CDS             455..1018
                     /locus_tag="VI520_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36362.1"
                     /translation="MLEVTLAVSVLAAVVVVGVLISVGNERQRQALDKLREDLRNWAL
                     GDLEVKRERAAREIQIMDPLAWFRDAARKATGVAWEMRAIAKVLEHPEAIVVASENGS
                     YLMFSPVRPDRVRRLLKAPSGRAQTGPLPSEMRLALRGRKRMESYELSALNAGIFFDL
                     EADKVWRMLTDKPLKSNQLWLYEFRSA"
     gene            complement(997..1533)
                     /locus_tag="VI520_00360"
     CDS             complement(997..1533)
                     /locus_tag="VI520_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36363.1"
                     /translation="MRPARRLALASLAVSTAALGLGFVRGKTEILILPLAISTALALI
                     GEARSSSWSPTLWFLAQAGLCALGRSGEVWGLISISCALAYWDLSAFHHRMRNAGHID
                     EPRSMLQRHLLYLLATMAVGFGLGIASVIVRIDGDFGGALLLGVIALAGLSQLLRRAQ
                     LPTTDLGSWERGQADRNS"
     gene            complement(1530..2792)
                     /locus_tag="VI520_00365"
     CDS             complement(1530..2792)
                     /locus_tag="VI520_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLF36364.1"
                     /translation="MVICALLVGGLTTFDSSLLALALPPLVYLAAAVVLRPTELQLQV
                     ERRVTPTSAKPGEIITVALQAENSDGRLEEFEIQDRVPQGLERIEGASSLICSMPFGS
                     RQQLSYRLLANTGLHTFTGARLKATDPFGLFEARLTVPGDRALLVRPAIQKLRRLYLR
                     PQRVGAQAGAYSARVGGPGIEFYGIRSYSPGDPWRWISWKVSGRFPGNLFINVFEQDR
                     MAEVGLIVDARSRSNAGTGEQSALAHSIQAAASFSELLLSQGNQVGLLVYGGHLDWTV
                     PRTGKVQRERILHALARASHGQSQIFDQLRHLPSRLFPPHTQLILFSPLLRTDVPDLL
                     RLRARRYPLLVISPDQVALEARSLLDRPDADLALRLARVEQAAISRRLAHAGVFRLLW
                     DTTVPFEQMTEMHLSRPAPWLASIGARL"
     gene            complement(2819..3781)
                     /locus_tag="VI520_00370"
     CDS             complement(2819..3781)
                     /locus_tag="VI520_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLF36365.1"
                     /translation="MSPDMNAVRMLAETVVAEVERAVVGKTELLNHLLACFLAGGHVL
                     LEDYPGLGKTLIAKSLASALGLEFRRIQFTPDLLPADITGTYVFSRNSDSFELRRGPI
                     FTNILLGDEINRASPRTQSALLESMQEHQVTLEGESIQLPDPFVVIATQNPIEYEGTF
                     PLPEAQLDRFMMRLQVGYPSIEQEQEVLNRRRTRQSEQLVISQVVRPEQVAAARQAVE
                     RVHVDSDIERYIVQLASATRRDPRVAVGASPRGALALMNSSRAWAAIQGRNYVLPDDV
                     KRLAKPALVHRLLLQPEFWLKKNAPEDVIEASLAAVPVPVLEGL"
     gene            complement(3771..4292)
                     /locus_tag="VI520_00375"
     CDS             complement(3771..4292)
                     /locus_tag="VI520_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36366.1"
                     /translation="MRAGWVVGALAVLIGLERLVEAAVVRFGIRPLGQVWAWITGRFG
                     QLPQAELWAVLVGLFGLLGLRALAGRAARPSAELRSQSHSQGSLQGWLELMENRTRGE
                     YFRWRLARRVASLADELDLPPPTNEPQIVAFLQAGRQLRTIRAQSTRDELKLDSAILV
                     EYLERTQEMQGVA"
     gene            complement(4307..5158)
                     /locus_tag="VI520_00380"
     CDS             complement(4307..5158)
                     /locus_tag="VI520_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HLF36367.1"
                     /translation="MSLLRSDRRLSLLYAAAAILGVAALASGISGVELRSGLRLGFTD
                     LSSPLDPTTDQSIDPPSEILRWLGAIVRFVFFALLPPSLLYLVVSPNARRRVLIQVLS
                     LLLVSYLVLRISPSLRAAVPALPSPQGPGAAGQAAATVLEGGLPPRWLVAGISSFIAV
                     GLLAAGYWVVRRASKVSDPRIGELRQALRALDRGQSDLESLIYQAYRTLCEVSGERRG
                     IRRAQAMTPREYQAALERAALPSGPLARLTALFEKARYGREKLGQEDEQVATAALRSI
                     LQEIELT"
     gene            5242..5514
                     /locus_tag="VI520_00385"
     CDS             5242..5514
                     /locus_tag="VI520_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36368.1"
                     /translation="MRISRPSWSPCLAQADALREAIAAWVEASEAARGFMVRMPVDTS
                     AALAPFHPGYFNEMQAAYERERAAREQYILANNALYDCMESNGLIP"
     gene            complement(5581..5652)
                     /locus_tag="VI520_00390"
     tRNA            complement(5581..5652)
                     /locus_tag="VI520_00390"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5618..5620),aa:His,seq:gtg)
     gene            complement(5689..6975)
                     /gene="hisS"
                     /locus_tag="VI520_00395"
     CDS             complement(5689..6975)
                     /gene="hisS"
                     /locus_tag="VI520_00395"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HLF36369.1"
                     /translation="MTAISSVKGTRDFYPEDMAFQRWLYASIRAVSEQFGYQEYDGPF
                     LERVELYAARSGDELVREQSFVFHDRSGELIALRPELTPSLARMIAARSKVLPRPIRW
                     WSFGPFWRYEQPQRGRSREFFQWNIDLLGVNSPEADAEIAAIAAAFLGRVGLAPDAVR
                     ILINDRRLAEQQLRSIGFPDAQHPSVFRLIDRRERMSPVDWEAHALVSGLTRNQLDQL
                     QVTLADREAWRNSEELSRFFRAAEALGAAQYLEYDSGVIRGLDYYTGIVWEARDTGRE
                     FRAILGGGRYDDLISAVGGDPVPGTGFAMGDVVIRLVLEKYDALPKLRTNPADLLIAS
                     FETESLEPALQLAARFRQAGLRVEWYPQPDRLPKQLKYADRQGIPWVAILGPEEISSQ
                     RVTIKNMRTVTQESVTLDEAVGRVKQLAAAQAQSTA"
     gene            complement(6985..>9339)
                     /locus_tag="VI520_00400"
     CDS             complement(6985..>9339)
                     /locus_tag="VI520_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLF36370.1"
                     /translation="FLRDQLDKSRSDQKVVLLDCPTIAAFDGARPLVGSSSGILESIE
                     GSRRGRFLICSTDLIGAALEGRQLTGEPSRGGLSQLVHQGLTTGDADLNLDGQVTVEE
                     LYEYIYDRSAAREAVSRGMPHRSSSPDLGEILIAKNPVWHPVELPPELREALHSPLAW
                     MRQGAVPELERMLTGQNKKLSLAAREALATLTTDQAPEIARSATAVLQAKVPSAPATA
                     PASTPSTPTPGTARRGVARIPTLGWLAGGLVLLFALGLAAGAVFGPKQEALPTEAVPS
                     PVSVQASPVVETASGTPQPEPTLAPEAPLPASIGMVPIPAGTYPIGSNLAVALAAYWI
                     DQFEVSNTDFAAYLDATNQPLPRYWVEDNIPSEMGDHPVRTVSWDLADSYCRWSGKRL
                     PSEAEWEVAARGPRGLQYPWGEQEGAVELPPGGTYPVGSISANRSVFGVYDLAGNVWE
                     WVDVPYSPLREGERVLRGGANNFPNDMTYRLIGNPEASNTVSDAGFRCAAEAVEVRVD
                     PNLFVTDEFANILSGWFQAAQPVREYFYGYHPTDFYHLQVSATEDCLAVRHELTLENF
                     VAEADIFQAKTETETGNYRHGLIIREAASEFYAFTVSPRTQVWQITRNSPNGMVILAE
                     GVDDSIRGLIRDEADKLTVIANGPELILFINGRLVGRVYDDDYRDGNIGFIVQTVDET
                     YAHSHFDRVFVWGLPANALPVTEIPSGAAAPTRISRAACAGSVSGDDVLESFFTYTVK
                     EGDTLSLIADLFGLTLVDVKGANGRRIQDPNVIVVGQVLIIPEQ"
BASE COUNT         1821 a         2883 c         2958 g         1677 t
ORIGIN      
        1 tggggagcaa accttgttca cctggccata cgatcctgag ttcgttccag gcacgatcag
       61 ttgggatggg gtgttgccag acgggaagag tgcagcgccc ggcgagtacg gggtgtatgc
      121 tacggcttgg gatttggctg gaaaccaggg tcaggatgca ggcgtcatcc gggttccagg
      181 gtcggacctt ctggcgccga tcggggagct cttggccaac acgccacttc gaggagttgg
      241 ccagccggat caggatccgc agggggcctc actctcaaga ccctcctcgc gaatctccag
      301 cagcctctgg ctgtggccga cgattggttg gatcggattg ttgggaggcg taacggtttc
      361 caagatgctc gatccacggc ccgctgccct gaatcggctg cgacaagagc tagcccaagt
      421 tcgcgcctgg caggccgaat gaggaggaag agccatgctt gaggtcaccc tggcggtcag
      481 cgtattggcc gcggtggtcg tggttggggt cttgatctcg gtcggcaacg agcggcagcg
      541 gcaggcgctc gacaaactgc gagaagacct gcgaaactgg gcgctgggcg acttggaggt
      601 caagcgggaa cgggcggcac gcgaaatcca gattatggat ccactcgctt ggtttcgaga
      661 tgctgcccgc aaggcgaccg gagtcgcgtg ggagatgcga gccatcgcta aggtgctcga
      721 gcatcccgaa gctatcgtgg ttgcctcgga gaacggcagc tacctcatgt tctcccctgt
      781 ccggcccgat cgggtgcgca gactgctcaa ggcgccctcg ggacgcgccc agaccggccc
      841 cttgccttct gagatgcgct tggcacttcg gggcagaaag cggatggaga gctatgaact
      901 ctcggcgctt aatgccggca tcttctttga cctcgaggcc gacaaggtgt ggcgcatgct
      961 caccgacaaa cccctgaaat ccaaccagct ctggctctac gaattccgat ctgcctgacc
     1021 cctttcccaa cttcccaggt cagtcgtagg cagttgagcg cgccgcagca actgagatag
     1081 tcccgccaac gcgatgaccc caagcagcag cgctccgccg aaatcgccat caatccgcac
     1141 gatgaccgag gcgatcccga gtccgaaccc caccgccatt gttgctagga gatagagcag
     1201 gtgccgctgc aacatcgatc tgggctcgtc gatgtgccct gcattgcgca tgcggtggtg
     1261 gaatgccgat aggtcccagt aggcgagcgc gcacgagatt gaaatgagcc cccacacctc
     1321 gcctgaccgg ccaagcgcgc acagcccggc ttgagccagg aaccagaggg tcggcgacca
     1381 actcgagctc cgggcctcgc caatcagagc gagggcggtc gaaatcgcca agggcaggat
     1441 caggatctcg gttttccctc tgacaaagcc gagcccgagc gcggcggttg agaccgcaag
     1501 cgacgcaagc gccaatcgcc gggccggcct cataaccgag cgccgattga ggctagccac
     1561 ggggcaggcc ggctcaggtg catttcggtc atctgctcaa atggaactgt ggtgtcccag
     1621 agcagtcgaa acactccggc atgtgccaat cgtcgagaga tggcggcctg ttccactcga
     1681 gccagtcgca aggccaggtc ggcatcaggc cggtccagca atgatctggc ctcgagcgca
     1741 acctgatcgg gactgatcac cagcagcgga tatcggcgcg cccgcaagcg aagcagatcg
     1801 ggcacatccg ttctgagcag cggactgaac aggatcagtt gggtatgcgg aggaaatagg
     1861 cggctgggca ggtgccgcag ttggtcaaag atctggctct gcccgtggct ggcccgggca
     1921 agggcgtgca ggatccgttc acgctggacc tttccagtcc tcggaacggt ccagtcaagg
     1981 tggccaccat ataccaggag cccaacctga ttcccctgac tgagcaagag ctccgagaat
     2041 gaggccgccg cttggatcga gtgagcgagc gccgattgct caccggttcc ggcattgctg
     2101 cgcgagcggg catctacgat cagtccgacc tcggccatgc ggtcttgttc gaacacattg
     2161 atgaagagat tgcctgggaa ccggcccgat actttccagc tgatccagcg ccaagggtcg
     2221 cctggggagt agcttcggat gccgtaaaac tcgattccgg gtccacccac ccgggccgaa
     2281 taggctcccg cctgggcacc cactcgctgc gggcgcaggt agaggcggcg aagcttctga
     2341 atcgccgggc gtaccaagag agcccggtct cccgggacgg tgagacgggc ctcgaagagg
     2401 ccgaacggat cggtcgcctt cagccgggcc cccgtgaagg tgtggagtcc ggtattcgcc
     2461 aaaagccgat aactcagctg ctgacgactg ccgaacggca ttgagcagat cagactagag
     2521 gcaccctcga ttcgctcgag cccttgaggt acccggtctt ggatctcgaa ttcttcgagg
     2581 cggccgtcac tattctcggc ctgcagcgcg acggtaatga tttctccagg tttggcagac
     2641 gttggggtga ctcgtcgctc cacttgcagc tggagttcgg tcggtcgtag gactacggcg
     2701 gccgccagat agaccagcgg cggcagggcc agggccaaca gggacgaatc gaaagtggtc
     2761 aggccgccga ctagcagcgc gcagatgacc aaacccagcg cggctggccg gcgcgggatt
     2821 agagtccctc gagtaccggt acggggacgg cggcgagact cgcctctatg acgtcctcgg
     2881 gtgcgttctt cttgagccag aattccggct gcaagagcag ccgatgaacc agggcaggct
     2941 tcgccagtcg cttaacgtca tcgggcagta catagtttcg gccctggatg gccgcccagg
     3001 cgcggctcga gttcatcagg gccaaggcgc cgcgcggact ggcgcctacc gctacccgcg
     3061 ggtcgcgccg ggtggcgcta gcgagctgga cgatgtagcg ctcgatatcg ctatccacat
     3121 gcaccctctc aaccgcttgc cgggcggccg ccacctgttc ggggcggacg acctgggaga
     3181 tcaccaattg ttcgctttgc cgagtgcggc gccggttgag tacctcctgc tcctgttcga
     3241 tcgagggata cccgacctgc aacctcatca tgaaccgatc gagttgggct tccggaagcg
     3301 ggaaggttcc ttcgtactcg attggattct gagttgcgat tacaacgaat ggatccggca
     3361 attgaatgga ctcgccctcc agggtaactt ggtgctcttg catggattcg agcagagccg
     3421 actgagtgcg gggagaggcc cgattgatct cgtcgccgag caggatattg gtgaagatgg
     3481 ggccacgccg aagctcgaaa gagtcggaat tgcggctgaa cacataagtc ccggtgatgt
     3541 cggcaggcag caggtccggg gtgaactgga ttctgcgaaa ctcaagtccc aaggcacttg
     3601 ccaggctctt ggcgatgagg gtcttgccga gtccggggta gtcctcgagc agcacgtgcc
     3661 ctccggctag aaagcaagcc agcaggtgat tgagcaactc tgtcttccct accacggccc
     3721 gctccacctc ggcgacgacg gtctccgcca acatccgcac cgcgttcatg tcaggcgaca
     3781 ccctgcatct cctgagtgcg ctcgagatac tcaaccagga tcgcggaatc gagcttgagt
     3841 tcgtctcgag tcgattgcgc tcggatcgtc cgcaactgtc tgccagcctg gagaaaggcc
     3901 acgatctgcg gttcgttggt cggtggtggc agatctagct cgtcggccag cgaagcaacc
     3961 cgccgggcca agcgccagcg aaaatactct ccacgggtcc ggttttccat cagctcaagc
     4021 cagccttgca ggctaccctg cgaatgagac tgggaccgga gctcggcaga ggggcgagcg
     4081 gcccggccgg caagggcgcg cagtccgagc aaaccaaata ggccgaccag aactgcccag
     4141 agctcagcct gaggcagttg accaaacctt ccggtaatcc aggcccaaac ctgaccgagg
     4201 gggcgaatgc cgaagcgcac caccgcggcc tcgactaggc gttcgagccc aatcaggact
     4261 gcgagcgctc cgactaccca ccctgctcgc atccaccgtg aggggatcag gttagctcga
     4321 tctcctgcag aatcgagcgg agcgcagcag tggcaacctg ctcgtcctcc tggcccaatt
     4381 tctcccgacc gtagcgggcc ttttcgaaca gcgcggtcaa gcgcgcaagg gggccgctcg
     4441 ggagagccgc ccgttcaagc gcagcctggt attcgcgggg cgtcatggcc tgggcccttc
     4501 ggatccctct tcgctcgccg gagacttcgc agagagttcg gtacgcctgg tagatcagac
     4561 tctcgaggtc ggattgcccc ctatccaatg cccgcagcgc ctgacgcaac tcgccaatcc
     4621 gcggatccga caccttgcta gcgcggcgca caacccaata acccgccgcc agaaggccaa
     4681 ctgcgatgaa gctgctgatc ccagccacga gccagcgcgg tgggagtcca ccctcaagga
     4741 cggtcgcagc cgcttgccca gctgcgccgg ggccctgagg ggaaggaagc gcgggaaccg
     4801 cggcccgcag tgaaggagaa atcctaagca ccaggtagct taccaacaac agcgatagga
     4861 cctggatgag aacccgtcgg cgggcgtttg gggagacaac taggtaaagg agggagggcg
     4921 gcaagagggc gaagaacacg aacctgacga ttgccccaag ccagcgaagg atctcggaag
     4981 gggggtcgat gctctggtcc gtcgtcggat caagaggaga gctaagatcc gtgaacccca
     5041 gccgcaggcc gctgcgaagc tccacgccgg agattccgct cgccagggcg gccacaccaa
     5101 gaatcgcggc tgcggcgtac aacagagaca gacggcgatc cgaccgaagc aggctcacct
     5161 cgcgtcgaag tatacccttc gcctaatcca ggggtctctc aggttgcgaa gcccggtgtt
     5221 ccgtttagaa ttctgtgaac tatgcgaatc agtcgcccgt cgtggtcgcc gtgtttggcg
     5281 caggcagatg ccttgcgaga ggccatagca gcttgggttg aggcttcgga agccgcccga
     5341 gggttcatgg tgagaatgcc ggtggacacc agtgcagccc tcgcgccctt ccaccctgga
     5401 tacttcaacg agatgcaggc ggcctacgag cgagagcggg ccgcccgtga gcagtacatc
     5461 ctggccaaca acgccctcta cgattgcatg gagagcaatg ggctgatccc gtgaggaggg
     5521 tcttcggcta aagaaacgga tggcgctgcc ggtgcgtgcg ggcagcgcca ctactttagg
     5581 ggtgcctgac gggtttcgaa cccgcagcct cctcatccac agtgaggcgc tctacccttg
     5641 agctacaggc accatgtaag gggattctac ggactggccc gaagaacgtc aagcagtcga
     5701 ctgagcctga gcggccgcta gctgctttac tcggccgacc gcttcgtcaa gcgtcacgga
     5761 ctcctgggtc acggttcgca tattcttgat ggttacccgc tgggagctga tctcctccgg
     5821 accgaggatc gcgacccatg gaattccctg ccggtcggcg tacttcagct gcttgggcaa
     5881 ccggtccggc tgcgggtacc actcgacccg gaggccggct tgcctgaagc gggcggccag
     5941 ctggagagcg ggctcgaggc tctcggtttc gaaactggca atcaacaagt cggcgggatt
     6001 tgtccgaagc ttcggcagcg catcgtattt ctctaatacg agccggatca ctacatcccc
     6061 catggcaaac ccggtccccg gaacgggatc tccgccgacc gcggaaatta gatcgtcgta
     6121 cctcccgccg ccgaggatcg cccggaattc tctccccgtg tcccgagcct cccaaacgat
     6181 tccggtgtag tagtcgagtc cgcgaatcac ccccgagtca tattccaggt attgagccgc
     6241 gccaagcgcc tccgcagcgc gaaagaaccg ggacaattcc tccgagtttc gccaggcctc
     6301 ccgatccgca agagtgacct ggagctgatc cagctgatta cgcgtcaggc cagagacgag
     6361 ggcatgcgcc tcccagtcca cggggctcat ccgttcgcgt cggtcgatca gccgaaatac
     6421 cgatgggtgc tgggcgtccg gaaacccgat cgaccgaagc tgctgttcag ccagccgccg
     6481 atcgttgatg agaatccgaa ctgcatctgg ggccagcccg acccggccaa ggaaagcggc
     6541 ggcaatggcc gcgatttccg cgtctgcttc gggcgagttc actcccaaga gatcgatgtt
     6601 ccactggaag aattcgcggg accgccctct ctggggctgt tcgtaacgcc agaatggccc
     6661 aaacgaccac cagcggatcg gccgtggcag aaccttgctg cgggctgcga tcattctggc
     6721 cagtgacgga gtgagttcgg ggcggagggc gatcagttct ccgctgcggt cgtggaagac
     6781 aaacgactgc tcgcggacca attcgtcgcc ggatcttgcg gcgtagagct cgacccgctc
     6841 gaggaacggg ccgtcgtact cctggtagcc aaactgctca gagaccgccc gaatcgaggc
     6901 gtacagccat cgctggaagg ccatgtcttc cgggtaaaaa tctcgggtcc ctttgaccga
     6961 agagatcgct gtcatccctg cgaactattg ctccgggatg atcagcacct ggccaaccac
     7021 gatcacattc ggatcttgaa tccgccttcc gttcgcgccc ttaacgtcca cgagggtgag
     7081 gccgaacagg tcggcaatca gcgagagagt gtcaccttct ttgacggtgt aggtgaagaa
     7141 actctcgagc acgtcgtcgc cgctcaccga gccggcgcag gcggctcgac tgatcctggt
     7201 cggggctgca gcgccgctgg ggatctccgt gacggggagg gcgtttgcgg gaaggcccca
     7261 aacgaagacc cgatcgaagt gggaatgggc gtaggtttca tctacagtct gaacaatgaa
     7321 tccaatgttc ccgtctcggt agtcatcgtc gtatacccgg ccgaccaggc ggccattaat
     7381 gaagaggata agctcgggac cgttggcgat gaccgtcagc ttgtccgcct catcccggat
     7441 cagaccgcgg attgagtcat ccactccctc ggccagaatc accatgccgt tggggctgtt
     7501 tctcgtgatc tgccagacct gagtgcgcgg cgagacggtg aaggcgtaga actcagaagc
     7561 tgcctcgcga atgatgagac cgtggcgata gttgccggtc tcggtctcgg tcttggcctg
     7621 gaagatatcg gcttcggcga cgaagttctc caaggtcagc tcatgccgta ctgccaggca
     7681 atcctccgtc gcgctgacct gcaggtggta gaagtcggtc ggatggtatc cgtagaagta
     7741 ttcgcggacc ggctgggcgg cctggaacca tccgctcaga atgtttgcga actcgtcggt
     7801 cacgaacagg ttcgggtcga cccgcacctc gacggcctcc gccgcgcagc ggaaacccgc
     7861 gtcggacacg gtgttgctgg cctcaggatt tccgatcagt cgataggtca tgtcatttgg
     7921 gaagttgttg gcgccgcccc gcaaaacgcg ttcgccctct cggagcggcg aatagggcac
     7981 gtccacccat tcccagacgt tgccggccag atcgtaaact ccaaagacgc tgcgattagc
     8041 cgaaatcgaa ccaactggat acgtcccgcc gggagggagc tccactgccc cctcttgctc
     8101 tccccacgga tactgcagcc cgcgcggtcc gcgggccgcg acctcccact cggcctcgct
     8161 cggcagtcgt ttcccgctcc accggcagta cgagtcggcc agatcccagg agacggtccg
     8221 caccggatgg tcacccatct cggagggaat gttgtcctcg acccagtagc ggggaagggg
     8281 ttggttggtc gcatccaagt aagccgcaaa gtctgtgttg ctaacttcga attgatcgat
     8341 ccaataggcg gcgagcgcaa ccgccaggtt cgatccaatc gggtaggtgc ctgcaggtat
     8401 aggcaccatc ccgatcgacg cgggtagcgg tgcctccggc gccagggtgg gctccggctg
     8461 aggggtgccg ctcgcggtct ccactaccgg actggcttgg accgacactg ggctgggcac
     8521 cgcttcggta ggaagagctt cctgtttcgg cccaaagacg gcaccggctg ccaggccaag
     8581 cgcgaagagc agaacaagtc cgcccgcgag ccacccaagc gttggaatcc gcgcaacacc
     8641 cctccgggcg gtgcccggcg tcggtgtgga gggagttgat gccggggcgg tggccggcgc
     8701 ggaagggacc tttgcctgaa gcactgcggt agccgatctt gcgatttctg gggcttgatc
     8761 ggtcgtgagg gtggccaatg cctcgcgggc tgccaacgat aacttcttgt tttggccggt
     8821 cagcattctt tctaattcgg ggaccgcacc ctggcgcatc caggccagcg ggctgtgaag
     8881 cgcttctcgc agctcgggag gcagttcgac tgggtgccac accgggttct tcgcgatcag
     8941 aatctccccg agatccgggc tactagaccg gtgaggcatg ccacgtgaga cggcctctct
     9001 ggctgccgag cggtcgtaga tgtattcgta gagttcctcg accgtcacct gcccatcaag
     9061 attgagatcc gcgtcaccgg tcgtgaggcc ctggtgaacc agctggctca atccaccccg
     9121 gctgggctct ccggtcagct gacggccctc gagggccgcg ccgatgaggt cggtcgagca
     9181 aatgagaaat cgacctcgac ggcttccttc gattgactcc aagatccctg aactgctgcc
     9241 caccagtggg cgcgctccgt cgaatgccgc gatcgtcggg cagtcgagca gcacgacctt
     9301 ctggtcggag cgagacttgt cgagctgatc gcgcaggaa
//
LOCUS       DATDPQ010000018         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1054629, whole genome shotgun sequence.
ACCESSION   DATDPQ010000018 DATDPQ010000000
VERSION     DATDPQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054629"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VI520_00405"
     CDS             complement(<1..483)
                     /locus_tag="VI520_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36371.1"
                     /translation="MSELLAEVRFFLQRLDALAILDLLLVTGVFFLVISLLRGTRAVV
                     LLRGMVLLIIIMVLLTSLLPLPAFSSLLTATLPALLFVIPVVFAPEIRRAFERLGRAG
                     TFFSLFSEPAEAERTVNLIVSAAERLAEIRYGALITIEREDRLDEYVETGVPLDARLT
                     T"
     gene            complement(574..>2616)
                     /locus_tag="VI520_00410"
     CDS             complement(574..>2616)
                     /locus_tag="VI520_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF36372.1"
                     /translation="IKESIQAREDSADMYIRRKDAGKAVDNWRHIARLDPRNLASRTR
                     LALTYERLGRTRDSALEDLSVASILASGGKIDRAKEAVQRSLAILPGDPDATRYLRLL
                     NQGKKLPAPPQPRGGTAPLRMAQVQEFLRKDTDQPLEPDEQLKDPETAAQGEALTILA
                     GILFEEPKDNGKEQGLGMARIASGQTAELKPSSAIYRYLAQAIDLQTRGHKEQAVKEF
                     IRAMETGLEHPAAHYNLAMLLKELGKIDEARKHLVASLGHPGLALGANLALGRLAKNK
                     NDIREATRYLLQALKLADGLTVAQGQSNELNQFYETLLASQTEGDKKQLAKIVDSTLK
                     FLSGPEWLKRLKQARAELDKKGEGGGVVPIAEMLEVGETDRVLKSMQRVDRMMDQGLY
                     ASALEESMLALDYAPTYLALHRRMAEIQIAAGRPEAGYAKLSVVAETHRVRGESAQAA
                     DVYMKILQFTPVDIPARTRLIDLLVQQDRGDEALTQSLELAGIYRDMAEIDMARKTLA
                     NSLRLSQQMATGNGWSLKILHMMGDMDLSRLDQRRALRVYEQIRTLDPGDDKARVNLI
                     DLNLRIGQEDQAAKELDSYLEFLVESGRGPEALNLLEEMAREHPGKQSLHARLAEAYK
                     AAGRTADAISQYDALGEVQLDAGQNEDAIRSIQAIIDLNPPDVEDYVELLRNLHSG"
BASE COUNT          430 a          812 c          848 g          526 t
ORIGIN      
        1 ggtggtgagc ctggcatcca agggcactcc ggtctcgacg tactcgtcga gccggtcctc
       61 gcgctcaatc gtgatcagcg ccccataccg aatctcggcc aaccgttcgg ccgcgctgac
      121 aatcagattc acagttcgct ccgcctctgc cggctccgag aaaaggctga agaaggttcc
      181 ggcccgcccc aggcgttcga aggctcgccg aatctcaggg gcaaagacga ccgggatcac
      241 gaacagcagg gcgggcaggg tggcggtcag cagggaggag aacgctggca gcggcagcag
      301 gctggtgagc agtaccatga tgatgatcag caggaccatc ccccgcagca gtactactgc
      361 ccgagtccct cgcagcaggc tgattaccag gaagaaaacc ccggtcacca gcagcaggtc
      421 gaggatggcc agggcatcca gtcgctgcag gaagaatcgg acttcggcta gtagttcact
      481 catcgcgaat ccgcgcggcg ggcgcgccta tgggtattgc tcatggtgct actcgccgcg
      541 cccgagaata cggccgacgg cggcagaggg ggcttagccg ctgtgcaggt tgcgcagcag
      601 ctccacgtaa tcttccacgt caggcgggtt aaggtcgatg atagcttgga tgctgcggat
      661 cgcgtcttcg ttctgaccgg cgtccagctg gacctcgccc aatgcgtcat actgggagat
      721 ggcgtcggcg gtccgcccgg cggccttata ggcctccgcc agcctggcgt gcagcgactg
      781 cttgccagga tgctcgcggg ccatctcctc taggaggttt agtgcctccg gcccgcggcc
      841 ggactccacc agaaactcca ggtaactgtc caactccttg gcggcctgat cctcctgccc
      901 gatgcgcaga ttgaggtcga tcaggtttac tcgggccttg tcgtccccag ggtcgagggt
      961 gcggatctgt tcatacactc gcagggcccg ccgctgatcg aggcgggaga ggtccatatc
     1021 ccccatcatg tgcaagattt tcagcgacca tccgttaccg gtcgccatct gctgcgacag
     1081 ccgcagcgag ttggccagag tcttgcgggc catatcgatc tcggccatgt cgcgataaat
     1141 cccggcgagc tcgagtgact gggtgagcgc ctcgtctccc cggtcctgct gcacgagcag
     1201 gtcgatcagg cgggtgcggg ccgggatgtc caccggagtg aactggagga ttttcatgta
     1261 aacatccgcg gcctgggcgc tctcgccgcg cacccgatgg gtctccgcga ccaccgagag
     1321 cttggcataa ccggcttctg gtcgtccggc cgcgatctga atttccgcca tccggcggtg
     1381 gagcgccaaa taggtaggag cgtaatccaa tgccaacatg gattcttcca gggccgaggc
     1441 gtatagacct tggtccatca tccgatcgac ccgctgcatc gacttgagta ctcgatcggt
     1501 ctcgccaact tccagcattt cggcgatggg gaccaccccg ccaccctcgc ccttcttgtc
     1561 aagctccgcc ctggcctgct tcagacgctt gagccactcc ggaccgctca ggaacttgag
     1621 cgtgctgtcg actatcttgg ccagctgttt cttgtcgcct tcggtctggg atgccaacag
     1681 agtctcgtag aactggttga gctcattcga ttggccctgg gcgacggtaa gaccgtcggc
     1741 caatttgagg gcctggagca aatagcgggt ggcttcccgg atgtcgttct tgttcttggc
     1801 caaccgtcct agggcgaggt tggcgcccag ggccaggccg ggatgcccca aggaggcgac
     1861 gagatgcttg cgggcctcgt cgatcttgcc cagctccttg agcagcatcg ccaggttgta
     1921 gtgggcggcg gggtgctcta gcccggtttc catcgcccgg atgaactctt tgactgcctg
     1981 ttctttgtgg ccgcgggtct gcaggtcgat ggcttgggcc aaatagcgat agatcgccga
     2041 ggaaggtttg agttcggccg tctggccgct tgcgatcctg gccatgccga gcccctgctc
     2101 tttgccgttg tcctttggct cctcgaagag gatcccggcc aggatggtca gcgcttcgcc
     2161 ctgagccgcc gtctctgggt ccttcagttg ctcgtccggc tccaggggct ggtcggtatc
     2221 cttccggagg aattcctgga cctgggccat gcgcagcggg gcggtgccgc cgcggggctg
     2281 tggtggggca ggcagcttct tgccctggtt cagcaaccgc aggtagcggg tcgcgtccgg
     2341 atccccgggc aggatcgcca acgaacgttg gacggcctcc ttggcccggt cgatcttgcc
     2401 gccgctggcg aggattgagg ctaccgacag atcctcgagg gccgagtcgc gggtccgacc
     2461 aagacgttca taggtcaatg ccaggcgggt gcgggaggcc aggttacgcg ggtcgagccg
     2521 ggcgatgtgg cgccagttgt ctaccgcctt tccggcgtcc ttgcggcgga tgtacatatc
     2581 ggccgagtcc tcgcgggcct ggatggattc tttaat
//
LOCUS       DATDPQ010000019        12643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2100793, whole genome shotgun sequence.
ACCESSION   DATDPQ010000019 DATDPQ010000000
VERSION     DATDPQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12643
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100793"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VI520_00415"
     CDS             complement(<1..794)
                     /locus_tag="VI520_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLF36373.1"
                     /translation="MGRAKLVLAVLAVAALACSFFPTAAVVAPTVEPAILPAAAPPAV
                     VVEAPSEPLFAPQGLVELYERVNPGVVTIYVFGPPHPDNIPLGQGSGFVIDTEGHIVT
                     NHHVVQGADEIEIDFASGFKAWATLLGTDPDSDLAVLKVEAPPTELVPIPLGDSDSVR
                     VGEQVVAIGNPFGLSGTMTVGVVSAIGRVLQSERQAPGGGAFSAGDLIQTDAAINPGN
                     SGGPLLNLSGQVIGVNRAITTDSFSASGSAVNSGVGFAVPINIVRRV"
     gene            complement(825..1706)
                     /locus_tag="VI520_00420"
     CDS             complement(825..1706)
                     /locus_tag="VI520_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HLF36374.1"
                     /translation="MEYKDYYQILGVGRQADEKEIKRVYRQLALKFHPDKNPGSEEKF
                     KEINEAYEVLGDPEKRAKYDRLGSSYRAWERAGGPGGFDWSQWVGGRPGGTRVEVDDL
                     FGGGFSDFFDSVFGLGSQRLRRGRDLEQPIAISLAEAYRGTTRTYQRNGRRLEVEIPA
                     GARSGTRIRLSGAGDGDPGRAGDLYLKVQVLDEPGVKRRGDNLHLEFQVDLYTAVLGG
                     EVELQTIQGPVLLRVPPGSQPGQTFRLRGRGMPKLGSPTHHGDLFAKLQVVLPAELGE
                     DERALFEQLAALHKRKP"
     gene            1800..2999
                     /locus_tag="VI520_00425"
     CDS             1800..2999
                     /locus_tag="VI520_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36375.1"
                     /translation="MNRAIIRKMPTSARPERVLIVDEDPDVLDLLSRQVLEPLGFRVS
                     TAVSAGEAIQQAVGSAPDLIVASLDLPGLSGKDLLVALRSQGIEVPVLVTAEAGREAD
                     AIQAFRLGARDYLVKPLREAEVLAAIERALKEVRLGHESEQLSVQLADSARQLEQRVR
                     ELTTIYGIGKAVTLITNQRELFDKLMQSSLNVTNADMGWVLLLDDDRGQLFLRAQLGM
                     PRALVGKLHQPWDDGVSSLVMLSAESLSIHSEGLEQFKIARFAKAALVVPIKARDQAI
                     GVITVAREQAKPFSEPNQRMLEAVADYASISLVNSRLFHALSGRAERLQEVVNETQTG
                     LQDQVAWVERVRKTLQGLHEQLPGLLESSENPSQRKRLQVLITDLQTLLKELDDMPHL
                     QSAPSSN"
     gene            3137..4663
                     /locus_tag="VI520_00430"
     CDS             3137..4663
                     /locus_tag="VI520_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36376.1"
                     /translation="MSQQSTTDELQRKLKGLEALTQVGRTVTALLDHSDVLTAVVEAA
                     VRLTGAEEGSLLLIDDDTGDLYMRASKNFDDEFARTFRLPVDDSLAGQVIESGEPVLL
                     DERTPQKIKTAYLVHSLIYVPLRVRGRTIGVLGVDNRQAGGTLTRQDVSIMVAMADYA
                     AIAIENTRLFNSSEAQRRKMETILARVENGVIVVDPENRLLIMNRSAREAFGVNGDWP
                     GRPVSDIIREPKLLSMLRISGKVPKREEVELGDGRVFSAQRTPIDGIGQAIIMQDITH
                     LKELDRIKSEFVTTVSHDLRSPLTAILGYVELIQRAGELNSQQEEFIRRVQLSVGQIT
                     NLVSNLLDLGRIEAGLDTAKEKTPVSVLARYALESMRGSASNKGLVLQSDLPDDLPLV
                     MGAPIRLRQMIGNLLDNAIKYTPPGGQVRIEANSEGDQIILRVYDSGPGVPPADQPYL
                     FDKFFRGSNVPQDLPGTGLGLSIVKSIVDSHDGRIWVDSTPGQGTTFTIVLPISDRES
                     "
     gene            5063..6112
                     /locus_tag="VI520_00435"
     CDS             5063..6112
                     /locus_tag="VI520_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HLF36377.1"
                     /translation="MQDLLGFEPALAAEVAGDGKLRSTRLGSLAFVDLETTGLAGGAG
                     TLGFLVGVGTFQPAGFRLRQYFLRDPAEEAAMLRLVKLDLEQMEGIVSFNGRVFDLPL
                     LEARSTIALRERWRLNEWPHFDLLYPSRRLWRKSLEDCRLATLEQHILQLTRADDDVP
                     GELIPGMYLDYLRTGDASDMRRVIYHNSVDILTLVGLASGVLDRYRRPDPEDLSGEEA
                     LAVARWHQASGRAGSAEAAYRVAVESAGKVELRAEALRRYTGHLKRAGQPKQALAAWN
                     SWHKLAPDDPTPCLELAKYYEWQAGSYQQALDWAEQARSSLARRPADWRRRQAESEID
                     HRVRRLRSKLRRGTS"
     gene            complement(6117..6710)
                     /locus_tag="VI520_00440"
     CDS             complement(6117..6710)
                     /locus_tag="VI520_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF36378.1"
                     /translation="MTPPASLFSTQELSSETWSDFAKLFAKHGGVQAGCWCMFYHRAG
                     PLRPAAESRLNWANRNRSDKRALVRGGSSHGILVYAGNQPVGSCQYGLKQELPRIDAG
                     RNYRKLELANDPGQLWRITCFFVDRDFRRQGIATLALRAALEAIQKKGGGVVEAYPVT
                     NPRAVPIWFGSVEMFEREGFQKTAQLGRSTLVMRRSL"
     gene            complement(6729..7310)
                     /locus_tag="VI520_00445"
     CDS             complement(6729..7310)
                     /locus_tag="VI520_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLF36379.1"
                     /translation="MRKAANLILFVVDASWSMAVAERMSATKGAILSLLTDAYQRRDR
                     VGLVVFQKDRALTVLPPTNSIQLAHRALADIPVGGKTPLSAGLSLALELFERERLSHP
                     EVLPLMILLTDGAGNVSMGDLPPQEEALLMADRFHDNAIRSVVINMEHAAFDQGLAHA
                     LADRLDAPCYTLQQLKADHLYRTVRHEINGTQE"
     gene            complement(7682..8737)
                     /locus_tag="VI520_00450"
     CDS             complement(7682..8737)
                     /locus_tag="VI520_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36380.1"
                     /translation="MLLALVLNAVNPRIGGVLIRGERGTAKSTAARALAALLPEVKVV
                     ADCRFSCDPDQPMTWCTECRERASAGERLPTAVFRTPFVDLPVSATEDRVVGTLDIER
                     AIKEGERHFEPGVLASANRGLLYIDEVNLLDDHVVDLLLDSAALGMNIIEREGISFTH
                     PSRYILVGTMNPEEGDLRPQLLDRFALSVDIRGLTDPWDRVAIMERNLAFETDPEGFR
                     EEWMEKELALSNKIEAGRRLVDSVTYHGRDLHAIAALTSALRVDGHRADLVILKAAKA
                     HAAFEGRSAIGETDIARAAELALPHRLRRGPFQQAEFQLADLEERLEQIRAEGMPAEP
                     EPKEEQEPPRKKVGARA"
     gene            8910..10244
                     /locus_tag="VI520_00455"
     CDS             8910..10244
                     /locus_tag="VI520_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLF36381.1"
                     /translation="MQDSEEDRTASGDPLKVAPVHPMDALLESQDYELEAPRRGEIRT
                     GTIARVTETDVLVDIGAKSEGIISARELEQLSPDQRALLGVGKEISVYVLRSGGGSAP
                     ELSLARAAEEQDWSTAEGLLSSKAVFEGEVSGYNKGGLIVSLGRLRGFMPASQVSLPR
                     RRRAEGDTPDQRWGKMVGEQIVSKVIEVDRQRNRLILSERSAARESREALKERLISEL
                     TVGEVRTGHIISLADFGAFVDIGGADGLVHVSEISWKRVDNPREVLKIGDEVQVKVLG
                     VDPERNRISLSIRELEANPWELIQEKYGEGKLVQGKITKLTKFGAFASLAGLEDYGIE
                     GLVHISELSDQRILHPSEAVQEGQDLTLRVLRVEPERRRIGLSLRRVDSADYVEQDWA
                     SAVQEQMQVEPVMDFDAELEAEAELAGLEEEPIQEVAAKQGEQLPAETEAEN"
     gene            10331..>12643
                     /locus_tag="VI520_00460"
     CDS             10331..>12643
                     /locus_tag="VI520_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HLF36382.1"
                     /translation="MADKMERFTQRARRVLSLAHEEAERLHHNYIGTEHLLLGLIREE
                     GGVAGRVLRNLGLDPARVKDMVERLTGVGRNAGSRIELAPGTEQVLQMAIEEARRMGH
                     HYIGTEHLLLGMIRQNEGVGMDVLRRLGTSPEQIRRETRRVLQEAPIRRATQASGRER
                     KEPQVKTPLVDQLATDLTGLAEEGKLDPVIGRQMEIERVIQILARRTKNNPALIGEPG
                     VGKTAIVEGLAQRIVAGETPEPLLGKRVLQLDVGSLVAGTMYRGQFEERLKRVIDELK
                     SSGAILFIDEVHMLVGAGSAGSSVDAANILKPALSRGELQVIGATTLDEYRKYIESDS
                     ALERRFQPVYVDEPTIEETIDILRGVRAPYENHHKLVITDEALEAATHLSSRYVTDRF
                     LPDKAIDLIDEAGSRVRMYKSPVARSLQETLQQLRESRQAHALAIEEARYDDAQELME
                     HTDELEEKLTKLRAGWERSEDSPRVTADDIAEVVAMWTGVPVMQIAQEESQRLLQMEE
                     VLHQRIIGQNEAIEAISKAVRRARAGLKDPHRPVGSFIFLGPTGVGKTELNKALAEFL
                     FGSEDALIQIDMSEFMERHSVSRLVGAPPGYVGYDDAGQLTEAVRRRPYSIVVFDEIE
                     KAHPEAHNMLLQIMEEGHLADARGRKVDFRNAIVVMTSNLGAELIMHQTELGFATKRD
                     EGQEERLAYQEMHKKLTDALRKEFRPEFINRVDSIIVFHALTMDQIKQIVDLELAKVA
                     TRLEAYSITLQASEAARVLLAELGYDPEMGA"
BASE COUNT         2481 a         3782 c         4120 g         2260 t
ORIGIN      
        1 acccggcgga cgatgttgat cgggactgcg aaccccacgc cggagttgac cgcgctgccc
       61 gaagcgctga agctgtcggt ggtgatagcc cggtttacgc cgatcacctg gccgctcagg
      121 ttgagcagcg gaccgcccga attgccggga ttgatggcgg cgtcggtctg aatcagatcg
      181 cccgcgctga aagccccgcc tccgggtgcc tgtcgttcag actgcagcac ccggccgatg
      241 gcggatacca ctcccacggt catggtaccc gacaggccga atggattgcc aatcgccacc
      301 acctgctcgc ccaccctcac cgaatcggag tcgcctaacg ggatggggac cagctcagtg
      361 ggcggcgcct caaccttcag cacagccaga tccgaatccg ggtcggtgcc aagcagggtc
      421 gcccaggcct tgaatccgga agcaaagtcg atctcgatct catcagcccc ttgaacgacg
      481 tggtgattgg tgacgatgtg cccctccgtg tcgatcacga agccggagcc ctggcccaag
      541 ggaatgttgt cggggtgagg cggcccgaac acatagatcg tgacgacgcc agggttcacc
      601 cgttcataga gctcaaccag gccctgcgga gcgaacaacg gctcagaggg tgcctccacg
      661 acaaccgcag gcggggcagc ggctgggagg atcgccggtt caacggttgg ggcaacgact
      721 gccgccgtgg ggaagaagct gcatgccagc gcagcgaccg ccagtacagc aaggactaat
      781 ttggctcgac ccatggtgtc ccttccttta tcaccggttt ccgatcaagg ctttcgctta
      841 tggagtgcgg caagctgctc gaagagcgcc cgctcgtctt cgccaagctc tgcaggcagt
      901 accacttgaa gcttggcaaa taaatcgccg tgatgagtgg ggctacccaa ctttggcatt
      961 ccccggccgc gcagtcgaaa ggtctgaccg ggctgactgc cgggcgggac tcggagcagc
     1021 actggaccct gaatggtctg aagttcgacc tctccaccca gaaccgcggt gtataggtcc
     1081 acctggaatt ccaggtgcag attgtcaccc cgccgcttga cccctggttc atcgagcacc
     1141 tgaactttga ggtagaggtc gcccgctctt cctgggtcac cgtcgcccgc cccggacaat
     1201 cgaattcgag tgccgctgcg ggcccccgcc gggatttcga cctccaggcg ccggccgttg
     1261 cgttggtagg tgcgggtcgt tccgcgatag gcttcggcca acgaaatggc aatcggctgt
     1321 tccaggtcgc ggccgcggcg gagccgctgg cttcctagtc caaagacgct gtcgaaaaaa
     1381 tccgaaaacc ccccgccgaa caaatcatcc acctcgaccc gggtgccgcc aggtctgccg
     1441 ccgacccact gagaccaatc gaaccccccc ggtccgccgg ctcgttccca ggcccggtag
     1501 gaactcccca accgatcgta cttcgccctc ttttccggat cgccgagaac ttcataggcc
     1561 tcgttgatct ctttgaactt ttcttcggaa ccggggttct tatccgggtg gaatttgagc
     1621 gccaactggc ggtagacgcg tttgatctcc ttctcgtctg cctgccggcc gacgcccaga
     1681 atctggtagt agtccttgta ctccatcagt ttgattctaa attgccgctg aaacccaagt
     1741 caagcaaagc tggcggatct aatggaactc ttatcgcagc cagcgcagag ggccaagcag
     1801 tgaaccgtgc tatcattcgc aagatgccca cttctgcccg accggagcgg gtcttgatcg
     1861 tggacgagga ccccgacgtg ctggacctgc tctcccggca ggtgctggag ccgctgggat
     1921 ttcgggtatc taccgcggtg agtgccggcg aggcaatcca gcaggccgtc ggctcggctc
     1981 cagacctgat tgtggccagt ctggacctcc ccggcctgag cggcaaggac ctgttggtcg
     2041 cacttcgctc gcaggggatc gaagtcccgg tgctggtcac cgccgaagca ggtcgggagg
     2101 cggacgctat ccaggccttt cggctggggg cccgcgacta tctggtcaag ccattgcgag
     2161 aagcggaggt gctggccgcc attgagcgag cgctgaaaga ggtgcggctg ggccatgaga
     2221 gcgagcaatt gtctgtccaa ctggcggaca gcgcccggca gctcgagcag cgggtgcggg
     2281 aactgaccac catttatgga atcggtaagg ccgtgaccct gatcacgaac cagcgcgaac
     2341 tattcgacaa gctgatgcag agcagtctga acgtcaccaa cgccgacatg gggtgggtgc
     2401 tgctcctgga tgatgaccgg ggccagctct tcctgcgggc ccaactcgga atgcctcgag
     2461 cgctggtagg aaaactgcac caaccctggg acgacggagt gagttcactc gtgatgctgt
     2521 ctgcagagag cctgagcatc cactccgaag ggctggagca gtttaagatc gcccgcttcg
     2581 ccaaggctgc cctggtcgtg cccatcaagg cccgggatca agcgattgga gtgataacgg
     2641 tagcccggga acaggccaag ccattcagcg agccgaacca gcgaatgctg gaagcggtcg
     2701 ccgactatgc gtctatatcc ctggtcaatt cccgcctctt ccacgcgctg tccggtcggg
     2761 cagagcggct gcaggaggtc gtgaatgaga ctcagaccgg tctgcaggac caggtggcat
     2821 gggtagagcg ggtgcgcaag accctacagg ggctgcatga gcagctgccc ggcctgttgg
     2881 agagctccga gaatccctcg cagcgtaaac ggctgcaagt cctaatcacg gacctgcaaa
     2941 ctctgctcaa ggaactggac gatatgcccc atctccagtc cgccccctcc tccaactgag
     3001 tacggtttcc caaaacgcga gtaccattct aggtccattc tggtaggtgc agggattctg
     3061 ctgctggcag gatagttgca tccctggacc cgcgatcagc aaacccggcc gaccgagtcc
     3121 gtaggtcccg cgtgacatga gccaacaatc gaccaccgac gagttgcagc gtaagctgaa
     3181 agggctggaa gcgctcaccc aggtcgggcg cacagtaacg gccctgctcg accacagcga
     3241 cgtgctcacc gcggtggtgg aggcggcggt gcggctcacc ggagccgagg aaggcagcct
     3301 gctgctgatt gatgatgaca ccggcgacct ctatatgcgg gcctccaaga acttcgacga
     3361 tgagttcgcc cgcacgttcc gcctgccggt cgacgactcg ctggccggcc aagtaattga
     3421 gagcggcgag ccggtgctgc tggatgagcg gacgccgcag aagatcaaga ctgcctacct
     3481 ggtccactcc ttgatctacg tgccgctgcg ggtgcgaggc cgaacgatcg gggtgctggg
     3541 ggtggacaac cggcaggcag gtggaaccct cacccggcag gatgtctcga tcatggtggc
     3601 gatggcggac tatgccgcaa tcgcgattga aaacacgcgc ctcttcaaca gcagcgaggc
     3661 gcagcggcgc aagatggaaa ccattctggc gagagtcgag aacggggtga tcgtggtgga
     3721 tccggagaat cggctgctga tcatgaaccg ctcggcccgg gaggctttcg gagtcaacgg
     3781 agattggccg gggcgaccag tctccgacat tatcagagag ccgaagctgc tttcgatgct
     3841 gcggatctcg gggaaggtcc ccaagcgaga agaggtggag ctcggcgatg gccgggtctt
     3901 cagcgcccag cggaccccga tcgatggcat cggccaggcg atcatcatgc aggacatcac
     3961 ccacctcaaa gaactagatc ggatcaagtc agagttcgtc acaaccgttt cgcatgattt
     4021 gcggtcgccg cttacggcca ttctgggcta tgtcgagctg attcaacggg ctggggagtt
     4081 gaattcgcag caggaagagt tcattcgccg ggtgcagctt agcgtcgggc agatcaccaa
     4141 cctggtcagc aacctgctgg atctgggacg gatcgaggcc ggtctcgata cggccaaaga
     4201 gaagacgccg gtctcggtcc tggcccgcta tgctctggag agcatgcggg ggagtgccag
     4261 caacaagggc ctggtgctgc aatccgatct cccagatgat ctcccgttgg tcatgggggc
     4321 cccgatccgg ctgcgccaga tgatcggcaa tctgctggat aatgcgatca agtacacacc
     4381 gcccggcggt caggtccgaa tcgaggccaa ttcagaaggg gaccagatca tcttgcgagt
     4441 gtacgacagc gggccgggcg tgcctccggc cgatcaaccc tacctgtttg acaagttctt
     4501 ccggggcagc aacgtgccgc aagatctgcc ggggaccggc ctcgggctgt ccatcgtgaa
     4561 gtcgatcgtt gacagtcacg atggccggat ctgggtggac tcgactccag gccaggggac
     4621 gacgttcacg atcgtccttc caatcagcga ccgcgaaagc tagccaaaaa tagcctccac
     4681 cggtgcggtg agcgacaacc cggtggaggc attcgccaaa ggaggagaca gtggtgagcg
     4741 taatggctct gtctcccgtc ctataatagt tccagtctcg agccggccgg aattgcaaaa
     4801 cgtcatcgct cccggatgac cttctgcacc attccagcca gggctaaggg cgatccaacc
     4861 gaatatggac agcaacctaa ggcgccgcct caaacggctg gagcggcggg gagcccggcg
     4921 ccgaccgccg gctacagccc cgcgggctga aggttcgaaa agatccgctc tgcctccagg
     4981 cgaagagctt gagactccgc taggagtcgc ttaccggctt gagagccgct ttccgctaag
     5041 ctaccaacat gggcgggccg ccttgcaaga cctacttggc ttcgagccag ccctggcggc
     5101 cgaggtggcc ggggatggca agctgcggtc gactcgtctg gggagcctgg ccttcgtgga
     5161 cctggagaca accggccttg ccggtggagc gggtactcta ggtttcctgg taggggtggg
     5221 gacttttcag cctgccgggt tccggctgcg gcagtacttc ctgcgggatc cggctgaaga
     5281 ggccgcgatg ctgcgcctgg tgaagctgga tctagagcag atggaaggca ttgtgagctt
     5341 caacgggcgg gtattcgatt tgccgctgct ggaagcgcgt tcaacaattg cattgcgcga
     5401 gcgctggcgg ctgaacgagt ggccacactt cgatctgctc tatccgtccc gtcggctgtg
     5461 gcggaaaagc ctcgaggact gccgcctggc gactcttgag cagcacatcc tgcaattgac
     5521 ccgagcggat gacgatgtgc ccggcgagct gattccaggt atgtacctgg attatcttcg
     5581 gaccggcgac gcttctgaca tgcgccgcgt gatttaccac aactcggtcg acatccttac
     5641 gttggttggg ctagcttcgg gagtgctgga ccgctatcgg cggccagacc cggaggacct
     5701 gtcgggcgag gaagcgctgg cggtcgcccg ctggcaccaa gcgtccggcc gggccgggtc
     5761 agccgaggcc gcctatcgag tcgcggtgga gagcgccggc aaagtggagc tgcgggccga
     5821 ggcgcttcgg cgatataccg gccacctcaa gcgggctggg cagcccaagc aagcgctggc
     5881 cgcttggaat tcctggcaca agctggcgcc ggacgacccg actccctgcc ttgagctggc
     5941 caaatactac gagtggcagg ccggctctta ccagcaggcg ctggactggg cagagcaagc
     6001 gcggtcctcg ctggcgaggc ggccagcgga ctggcggcgg cgccaggcag agtcggaaat
     6061 cgatcaccgg gtgcggcgac tgcgcagcaa gctgcggcgg ggaacgagct gaccgttcaa
     6121 agcgagcgtc tcatcaccag cgtgctgcgg ccaagctggg cggtcttctg aaagccttcc
     6181 cgttcgaaca tttcaaccga tccaaaccag attggaacag cccgtgggtt ggtgaccgga
     6241 taggcctcca cgactccgcc gccctttttc tgaatcgcct ccagcgcggc ccgcagagcg
     6301 agggtggcaa taccctgtcg gcgaaagtcc cggtcgacga agaaacatgt gattcgccat
     6361 agttggccag gatcgttcgc cagctcaagc ttgcggtagt tgcgtccggc gtcgatccta
     6421 ggcagttctt gcttaagacc gtactggcac gagcccaccg gctgattccc ggcgtagacc
     6481 agaatcccgt gcgaactccc tccccggact agggcgcgtt tgtcgctccg gttccgattc
     6541 gcccaattga gccggctctc ggccgctggg cgcagcggcc cggcccgatg atagaacatg
     6601 caccaacagc ccgcctgtac cccaccgtgc ttggcaaaga gcttggcaaa gtcactccag
     6661 gtctccgaag aaagttcttg agtgctgaag agcgaggctg gtggggtcat catccgcggc
     6721 tggcggggct actcctgggt gccgttgatc tcatgccgca ccgtgcgata caggtggtcg
     6781 gccttgagct gctggagggt gtaacacgga gcgtccagcc gatcggccag cgcatgagca
     6841 aggccctggt cgaatgcagc atgctccatg ttgatcacta ccgatcggat cgcgttgtca
     6901 tggaaccggt cggccatcaa cagtgcctct tcttgaggcg ggaggtcacc catagacacg
     6961 ttgcccgccc cgtcggtcag caggatcatc agcgggagca cctcgggatg actcagccgc
     7021 tcgcgctcga agagctccag cgccaagctg agcccggccg aaagcggggt cttgccgccg
     7081 accggaatat cggcgagcgc ccggtgggcg agttgaatcg agttggttgg gggaagcaca
     7141 gtgagggccc ggtctttttg aaacactacc aggcccaccc ggtcgcgacg ctggtaggcg
     7201 tccgtcaata gtgacaggat ggctcccttg gtcgccgaca tgcgttcggc gaccgccatc
     7261 gaccaggaag cgtcgacgac gaagaggatc aaattggccg ccttacgcac ccggaccttc
     7321 cgctgcaggt cggtgggctg gatcgcaaag gcgacctgct tgcgctgctt ggccctccgt
     7381 ttttggtggg gggccgcggc ccggatggtc gcgtcgaagg cgatgtcggt agttgactcg
     7441 gtgaccggcc gagcagaaat gtagcggccg cgcttccgat cagagcgagt ctgggtacgg
     7501 cgcccgctct gcttgcgggt cagtcgatcg attgggtttt cgatgcggcg agggatgaag
     7561 gagtcgccgg ttttgacttt gtcgccgcct tcccaccagc gggcctgcag tgagtcgacg
     7621 acggtctgct caaccggctt ggtctgtccg aggtctttgg tgtgctgttc ggggtcgcgg
     7681 gctacgcccg ggcgccgact ttttttcggg gcggctcctg ctcttccttc ggctccggct
     7741 cggccggcat gccttcggcc cggatctgct cgaggcgctc ctccagatcg gcgagttgaa
     7801 actccgcttg ctggaagggg ccgcggcgca agcgatgtgg gagggccagt tctgcggcgc
     7861 gggcgatgtc ggtctctccg atcgcgctgc ggccctcgaa ggcggcatgg gccttggcgg
     7921 ccttcaagat gaccagatcg gcccgatgcc cgtccacccg cagggcggaa gtgagggccg
     7981 cgatggcgtg caagtctcgc ccatggtagg tgaccgaatc cacgagtcgg cggccggcct
     8041 cgatcttatt ggagagcgcc agctccttct ccatccactc ttctcggaag ccctccggat
     8101 cggtctcgaa tgccaggttg cgctccatga tggccacccg gtcccacgga tcggtcaatc
     8161 cccggatgtc gaccgagagc gcgaagcggt ccaataactg gggacgcagg tcgccctcct
     8221 ccgggttcat cgtgccgacc aggatgtaac gcgagggatg ggtgaatgaa atcccttcgc
     8281 gttcaatgat gttcatgccc agggcggccg aatccagcag cagatccact acgtggtcgt
     8341 ccagcagatt gacttcgtca atatagagca aaccccggtt ggccgaggcc agcacccctg
     8401 gttcaaagtg gcgctcgcct tccttgatgg cgcgctcgat atccagggtg cctaccaccc
     8461 ggtcctccgt ggcggagacc ggcagatcga caaaaggagt acggaacacg gccgtcggca
     8521 gccgttcgcc ggccgacgcc cgctcccggc actccgtgca ccaagtcatc ggctggtcgg
     8581 ggtcgcagct gaaccgacaa tccgccacga ccttgacttc ggggagcaag gcggccaacg
     8641 cccgggcggc ggtggatttc gcagtccccc gctcgcctcg gatgagcact ccgccgatcc
     8701 gcggatttac ggcgttcaag acgagcgcaa ggagcatgcg ctcttgtccg acaatggcgg
     8761 taaaagggta aaccggcgcc atggcttggc tgtctcttgg ccgttagtac tcggccgcgc
     8821 ggcattatac cccctcgaga atcgaggcag gttgccaaag ctcattccta gcctttataa
     8881 tcaaccttct ggcacactca tgagcaacga tgcaagactc tgaagaagat cgaaccgcaa
     8941 gcggggatcc gctgaaggtg gctccggtcc acccgatgga cgccctgctc gaatcccagg
     9001 attatgagct ggaagctccg cgccgcggcg aaatccgaac tgggacgatc gcccgggtca
     9061 ccgagaccga cgtgttggtc gacatcgggg cgaaatcaga gggcatcatc tcggccaggg
     9121 aactggagca gctcagcccg gatcagcgag ccctgctcgg ggttggcaaa gaaatcagtg
     9181 tctatgtcct gcgatcgggc ggcgggagcg cgcctgagct ctcgctggcc cgagcggcgg
     9241 aagaacaaga ttggtcgacg gctgaagggc tgttgagcag caaggccgtt ttcgaagggg
     9301 aagtgtccgg ctataacaaa gggggactga tcgtgtcttt gggtcggttg cggggcttca
     9361 tgccggcctc ccaagtcagc ctgccgcgcc ggcgacgggc cgagggggat actcccgacc
     9421 agcgctgggg caaaatggtg ggcgagcaaa tcgtcagcaa agtaatcgaa gtggaccgac
     9481 agcgaaaccg gctaatctta tccgagcgat cggcagcccg cgagtcgcgg gaggctctta
     9541 aggaacggtt gatttcagaa ttgacggtgg gcgaggttcg gactgggcac attatcagcc
     9601 tggctgactt tggcgccttt gtggacatcg gcggcgcgga cggactggtg catgtctcgg
     9661 agatttcctg gaaacgggtg gacaaccccc gggaagttct gaagattggt gacgaagtgc
     9721 aggtgaaggt gctcggagtc gatccagagc gcaaccggat cagcctctcg atccgtgagc
     9781 tggaggccaa tccctgggag ttgatccagg aaaaatatgg cgagggaaag ttagtgcaag
     9841 gtaagatcac caaactgacg aagttcgggg cttttgccag cttggccggc ctcgaagatt
     9901 atgggatcga ggggctagtt cacatctctg agctgtccga tcaacggatc ctgcacccca
     9961 gcgaggcggt gcaggaaggc caggatttga cattgcgggt gctgcgggtg gagcctgaac
    10021 ggaggcgaat cggcctgagc ctgcggcggg tcgactcggc agactatgtc gaacaggatt
    10081 gggcgtccgc ggtccaagag caaatgcagg tagagccggt catggatttc gatgccgaac
    10141 tcgaggcgga agcggaattg gctgggctcg aggaggaacc aatccaagaa gtcgcggcta
    10201 aacaagggga gcagctcccc gccgagaccg aggcggagaa ttgaccggat aggactagct
    10261 acagggagtg ccaaagagga ctctttggta gaatccgatc agtcagtaat catctaggag
    10321 aaaacagttc gtggccgaca agatggagcg cttcacccaa cgggcccgac gagtgctcag
    10381 cctggcgcac gaagaggctg agcggctgca tcacaactac attggcaccg agcacctgct
    10441 gctcgggctc atccgggagg aggggggcgt agccggccga gtgctgcgca acctggggct
    10501 ggaccctgcc cgagtcaagg acatggtcga acggctgacc ggggtcggcc gcaacgcagg
    10561 cagccggatc gagttggctc ccggtaccga acaagtactt cagatggcga tcgaagaggc
    10621 ccgccggatg ggccatcact acatcgggac cgaacacttg ctgctgggca tgatccgcca
    10681 aaacgaaggc gtggggatgg acgtgctgcg ccggctaggg accagccctg agcagatccg
    10741 gcgggagact cgccgggtgc tgcaggaagc ccccattcgc cgagccaccc aggcctccgg
    10801 ccgagagcga aaagagccgc aggtgaagac ccccctggtg gatcagctgg cgaccgacct
    10861 caccgggttg gccgaagaag gcaagctgga cccggtcatc gggcgccaga tggaaatcga
    10921 gcgggtgatc cagatcctgg cccggcggac caagaataac ccggccttga tcggcgaacc
    10981 gggcgtcggc aagaccgcga tcgtcgaggg cctggctcaa cggatcgtgg ccggggagac
    11041 tcccgaaccg ctgctcggga agcgggtgct gcaattggac gtcggctcgt tggtcgccgg
    11101 cacgatgtat cgcggccaat tcgaagagcg gctcaagcga gtgattgacg agttgaagtc
    11161 ctcgggggct atcttattta tcgatgaagt tcacatgctg gttggcgccg gctcggcggg
    11221 gtcctcggtg gacgcagcca atattctgaa gccggcgctt tcacgtggcg agctgcaagt
    11281 gatcggcgcg acgaccctgg atgaataccg caagtacatc gagagcgact cggcgctgga
    11341 gcgccggttt caaccggtgt atgtcgacga acccaccatc gaggagacga tcgacatcct
    11401 gcgcggggtg cgggctccct atgagaatca ccacaagctg gtgatcaccg acgaggccct
    11461 ggaggccgcc acccaccttt cctcccgcta cgtgaccgat cgttttctgc cggacaaggc
    11521 cattgacttg atcgatgagg cgggctcccg ggtgcgcatg tacaagagcc cggttgcccg
    11581 ctcgctgcag gagaccctcc agcaattgcg ggagtcccgc caagcgcatg ccctggcgat
    11641 cgaagaggcc cgctacgatg acgcccaaga gctgatggag catacagacg agctcgagga
    11701 gaaactcact aaactgcggg caggctggga gcggagcgag gacagcccac gggtcaccgc
    11761 cgacgacatc gccgaggtcg tggcgatgtg gaccggcgtc ccggtgatgc agattgccca
    11821 agaagaatca cagcgcttgc tgcagatgga agaggtgcta caccagcgga tcatagggca
    11881 aaacgaagcg atcgaagcga tttccaaggc cgtgcgccgg gcgcgggccg ggctcaagga
    11941 tccccaccga ccggtcggtt cgtttatttt cctgggcccc accggggtcg gcaagaccga
    12001 gttgaacaag gccctggccg agtttttgtt tggtagcgaa gacgccctga ttcagattga
    12061 tatgtcggag ttcatggagc ggcactccgt tagccggctg gtcggggctc cccccggcta
    12121 tgtgggctat gacgacgcag gccagttgac cgaggcagtg cggcggcgcc cgtactcgat
    12181 cgtggtgttt gacgaaattg agaaggccca tcccgaagct cacaacatgc tgctgcagat
    12241 catggaagaa ggtcacctgg ccgacgcgcg gggccgcaag gtcgatttcc gcaatgccat
    12301 tgtcgtgatg acctccaatt tgggggccga actgatcatg caccagaccg aactgggttt
    12361 cgcgaccaag cgggatgaag ggcaggaaga acgcctggct tatcaggaaa tgcacaagaa
    12421 gctgaccgac gcgctccgca aggagttccg tccggagttc attaatcgag ttgactcgat
    12481 cattgtgttc catgccctga cgatggacca gatcaagcaa atcgtcgatc tggagctggc
    12541 caaggtggcc acccggctcg aggcctacag catcacgctc caggcctccg aggcggcccg
    12601 cgtgctgctc gccgagttgg gctacgatcc ggaaatgggt gcc
//
LOCUS       DATDPQ010000020         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1317154, whole genome shotgun sequence.
ACCESSION   DATDPQ010000020 DATDPQ010000000
VERSION     DATDPQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317154"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..325
                     /locus_tag="VI520_00465"
     CDS             107..325
                     /locus_tag="VI520_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36383.1"
                     /translation="MSSRFSLTRVAAACLAVLIPALAFGCAGAPDGLQVGDRAPRFEL
                     PAVGGERVALGDYTGQRPVLLFFHMAVG"
     gene            335..694
                     /locus_tag="VI520_00470"
     CDS             335..694
                     /locus_tag="VI520_00470"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLF36384.1"
                     /translation="MQQVVDLQNSPELQSLDVALFSIAFDSLAEMAPEAESLGITVPM
                     LSDTDHSVSEVYDVLQWAIASGEPGHTFVLVDERGEVAWIRDYGSPENANPTMYVDPG
                     EIAEQVRAALGTEGPDY"
     gene            complement(704..2077)
                     /locus_tag="VI520_00475"
     CDS             complement(704..2077)
                     /locus_tag="VI520_00475"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HLF36385.1"
                     /translation="MSTLLVKNAELLLTMDPPAGRIAGGGLFVRDNVIEQVGPSEGLP
                     TEADRVIDASGMLVLPGLINTHHHLYQTLTRALPGAQDVELFAWLTRLYPVWGEMDDG
                     AVYASALIGMAELLLSGCTTTTDHLYIYPNGSSLDATIRAAQEIGIRFHPTRGSMSLG
                     KSKGGLPPDHLVEVEADILRDCRRVIERYHDPRPYSMLRVGLAPCSPFSVTADLMRET
                     AALARSSKQVTLHTHVAETLDEERFCLEQFGARPAEYMRRLGWVGPDVWWAHAIWLND
                     EEIQMLAETGTGVAHCPSSNMRLGSGIAKIREMRDAGVKVGIAVDGSASNDGNDVLLE
                     TRMALLLQRVGKGAAAFAVQDALELATLGSAAVIGRDDLGRLAPGKAADLIGYRLDRL
                     EFAGGAVHDPVAALLLCTAKGVDLSVINGRIVVEDRQLLGFDLPGAIAKHNSIAAAMA
                     ARHPLPS"
     gene            complement(2088..>2592)
                     /locus_tag="VI520_00480"
     CDS             complement(2088..>2592)
                     /locus_tag="VI520_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLF36386.1"
                     /translation="ALFSHLESSLNVIWNAPRRAVWSRRLRGLLGVLILGTLFILAIA
                     VSALPALPFLDQDNPRLTVLDLGIGVVAEILLLWFIYRWLPNTTVRFVSAVAGAVLAA
                     LLWEAAQFGFRVYLTSGLANFGAVYGTLASVIGLILWAYLTGIILFLGAEFSAALQRE
                     FWPRPSS"
BASE COUNT          485 a          868 c          815 g          424 t
ORIGIN      
        1 ctcaaggccc gctacgccag cggcgagatt tcgaaggatc agtttgacaa gattcgacgc
       61 gacctcgcgc cctgaaaagg gtttcccaag gcatggagcg aaaccgatga gctcgagatt
      121 cagtttgacc cgagttgcgg ccgcctgcct ggccgtcctg atcccggcac tcgccttcgg
      181 ctgcgccggc gcccccgacg gcttgcaagt tggcgatcgg gcgcccaggt tcgaactccc
      241 ggcggtgggt ggcgagcgag ttgctctggg cgactacacc ggccaacggc cggtcctgct
      301 cttcttccat atggccgtgg gctgaccgcc ctgcatgcag caggtcgttg acctgcagaa
      361 cagcccggag cttcaatccc tcgacgttgc cttgttcagt atcgcctttg actcccttgc
      421 cgagatggcg ccggaggccg agtctttagg gatcacggtc cccatgctca gtgacaccga
      481 ccacagcgtg tcggaagtgt atgacgtgtt gcagtgggcg attgccagcg gcgagcccgg
      541 ccacactttt gtgctggtcg atgagcgagg ggaagtagcc tggatccgcg actacggctc
      601 accggaaaac gctaacccca cgatgtacgt ggaccccgga gagatcgcgg agcaggtccg
      661 ggccgccctt gggaccgagg ggcccgacta ctaagcctag cgatcagctc ggcagcgggt
      721 gtctggcggc cattgccgcg gcgatcgagt tgtgcttcgc gatcgcgcct ggcaggtcga
      781 accccagcag ctgtcggtcc tcgaccacga ttcggccatt gatcacggag aggtcgaccc
      841 ccttggcggt gcacaagagc agcgcggcca ccgggtcgtg aaccgcccct ccggcgaact
      901 cgagccgatc cagccgatag ccgatcagat cggccgcctt gcccggggcg agccggccga
      961 gatcgtcacg cccgatcacc gccgccgaac cgagggtggc cagctcgagg gcgtcctgca
     1021 cggcgaaggc cgcggccccc ttccccaccc gctgcaagag cagcgccatt cgggtctcga
     1081 gcagcacgtc gttcccgtcg ttcgaggccg atccatccac ggcaatccca accttcaccc
     1141 cggcatcccg catctcgcgg atcttcgcga ttccagagcc aagccgcatg ttggagctcg
     1201 ggcagtgagc caccccggta ccggtctcgg ccaacatctg gatttcctca tcgttgagcc
     1261 agatggcgtg cgcccaccag acgtccggcc cgacccagcc gagccgccgc atgtattcgg
     1321 ccggccgggc cccgaactgc tccaggcaga atcgctcctc gtctagagtc tcggcaacat
     1381 gggtgtgcag ggtgacctgc ttggacgatc gggcaagcgc cgccgtctcc cgcatcagat
     1441 cggccgtcac cgagaatggc gagcacggcg ccaatcccac ccgcagcatc gagtaaggcc
     1501 tcgggtcatg gtagcgctcg atcactcgcc ggcagtcacg caagatgtcg gcctcgactt
     1561 caaccaggtg atccggcggc agtccaccct tcgatttccc caggctcatc gatccgcgag
     1621 tgggatgaaa tcgaatgccg atctcctggg ccgcccggat ggtcgcgtcc agcgagctgc
     1681 cgttgggata gatgtacaaa tggtcggtgg tggtggtgca gccgctcaag agcagttcgg
     1741 ccatcccgat gagcgcggag gcgtagaccg cgccgtcgtc catttccccc cacactggat
     1801 acaagcgggt caaccaggcg aaaagctcga cgtcctgggc gcccggcaga gctcgggtga
     1861 gggtctggta caggtgatgg tgggtgttga tcaggccagg caggaccagc atccccgagg
     1921 cgtcgatgac ccggtcggcc tcggtcggga gcccctcgct gggtcccact tgttcgatga
     1981 cgttatccct cacaaacaat ccgccgccgg caatccggcc cgctggcgga tccatcgtca
     2041 gcaacaactc cgcgttcttc accagcaagg tggacattcg acgtaggcta cgaacttggc
     2101 cggggccaga actcacgctg gagggccgcg ctgaattcag cccccagaaa caggatgatc
     2161 ccggtcagat acgcccagag gatgagaccg atgaccgagg ccagcgtccc atacaccgca
     2221 ccgaagttgg ccagtccgga ggtcagatac actcggaacc cgaactgggc cgcttcccac
     2281 agcagggcgg ccagcaccgc tccggcgacg gccgagacaa agcgcaccgt tgtgttaggc
     2341 agccagcggt agatgaacca caagagcaga atctccgcta ccaccccgat cccaaggtcg
     2401 agcacggtga gccgcggatt gtcttgatcg aggaatggca gcgccggcag cgcactcact
     2461 gcgatcgcga ggatgaacag ggtgcccagg atcagcactc cgaggaggcc gcgcaaccga
     2521 cgcgaccaca cggcccggcg gggggcattc cagatcacgt ttaagctgga ttccaggtgg
     2581 ctgaacagcg cc
//
LOCUS       DATDPQ010000021         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1577404, whole genome shotgun sequence.
ACCESSION   DATDPQ010000021 DATDPQ010000000
VERSION     DATDPQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577404"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VI520_00485"
     CDS             complement(<1..825)
                     /locus_tag="VI520_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36387.1"
                     /translation="MTERRGLDKPAGGLSSVRAAAIGLAAFGLVERIGLWLVYAPASF
                     GDTPSYFRLAAALSESGLSAYDATRVPGYPALIGLLGQDPKLVWLFQMAAGWGISMLL
                     FWIGWVTTGSLGPGLVAGMAYNLIPGQILFEANLLSETLTAFLVILGLALWVALERET
                     GRSRAPGTGPGESAGQDRELVLAGLLGVTSALAGMVRPLFFVFPAWLFAFVVSKDAHE
                     RTHRIVRAIAFGVGPVLILGGWLNWVNSTYGMLSPTTMTGYHLVQHTGEYFEYLPDE"
     gene            complement(822..1892)
                     /locus_tag="VI520_00490"
     CDS             complement(822..1892)
                     /locus_tag="VI520_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF36388.1"
                     /translation="MKSCAALVELGHQVTLWLPAVRPEPGWEELAAHYGIQVRFPIRR
                     LRSLRALRHYDFCLRAVLAGRRSGAELLYVWPYQAAAIGSAWGVPTVLEVHDRPAGRM
                     GPRLFATFLRGRGARRALVTTEALRGWLAAHYGSERIDPLAAISPNGVDLDRYRGLPD
                     PNQARAELGLPDRFTAGYTGHLYAGRGLELMLTLAASMPDVQWLWAGGEPEAVIRWQA
                     RLQREGIENLKLLGFVPNERLPLVQAACEVLLMPYERRIAVSSGGDTAATASPMKLFE
                     YLATGRAILASDLPVLREILNETNAQLLPPDEPEVWREALLRLKKYPERRARLGEQAR
                     QDAQRYSWTERARRALEGLEIG"
     gene            complement(1957..>3286)
                     /locus_tag="VI520_00495"
     CDS             complement(1957..>3286)
                     /locus_tag="VI520_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLF36389.1"
                     /translation="PLGRMLRNPLMQRVMRNSGYLFTGSGLSAAMSMLQGILAARLLG
                     VQAFGVLGIITVFASVINRLTSFRMSELVVNYVGEFSSRDEPARAAATVKAAAAAEAI
                     SSVLAFGLIALLAPVGARYLAKDPSIVGLFTLYGSMVLAHLVVETSAGVLQYLDEFRL
                     QAWIQVGQSGLTLALILAAVLAKAGLNAVVWAYLVGKVAWAIAITLAAWIAARRRWGP
                     GWWRAPLSSIFDRRREMARFALSTNLTGTLTLVTRDSEVLWLGALTSPLQVGYYKVAL
                     AILNIVLIPVQPLISTTYREVAREVATRRWENVRYLLRSGSLIAAAYSLAAAGGLAIF
                     GVWVVSLWGSEFLPESYLALLVLLPGVIVVSVFYWNRSVLLPLGLPEYPTKVTLAAAL
                     AKVVLIFLLVPRLGAVGMAISLGSFFIGTAALMVWKTLREIQRKAALSPA"
BASE COUNT          645 a         1129 c         1001 g          511 t
ORIGIN      
        1 ctcgtccggg agatattcaa agtactctcc ggtatgctgg accaggtggt agccggtcat
       61 ggtcgtgggg gagagcatcc cgtaagtcga attgacccag ttcagccatc ctcccaagat
      121 caagaccggc ccgacaccga aggcaattgc ccgaacgatc cggtgagtcc gttcgtgagc
      181 gtccttggaa acgacgaagg cgaacagcca ggctgggaac acgaagaaaa gcggccgtac
      241 catccctgcc agtgcggaag tcactccaag caggccggct aagacgagtt cccgatcttg
      301 gcccgcgctc tcacccggcc cggtacccgg agcccggctg cgcccggtct cccgctccag
      361 tgcgacccag agcgccaggc ccagaatcac aagaaatgcg gtcagggttt cgctcaacag
      421 gttcgcttcg aacagaattt gaccggggat caggttgtag gccatgcccg cgaccagccc
      481 cggaccgagg ctcccggtcg ttacccagcc gatccagaac aacagcatcg agatccccca
      541 ccccgcggcc atctggaaca gccagaccaa tttgggatcc tggcccaaca atccaatcag
      601 tgcggggtag cccggtactc gggtcgcgtc gtaggccgag agtccagatt cgctcaaagc
      661 ggccgccagt cgaaaatacg agggcgtatc gccgaaggag gccggggcgt agaccagcca
      721 caggccgatc cgctcgacca ggccgaaggc ggccagaccg atggccgccg ctcgaacgct
      781 actcaatcca ccagccggct tgtccagacc ccgtcgttca gtcatccaat ctccagccct
      841 tccaacgccc ggcgggcccg ctcggtccac gagtagcgct gcgcgtcctg ccgggcttgc
      901 tcgccgagcc gcgccctccg ctccggatac ttcttcaatc tcagcagcgc ctctcgccag
      961 acctccggtt cgtccggagg cagcagctgg gcgttcgttt cattcaagat ctcccgcagc
     1021 accgggagat cgctcgccag gattgccctg ccggtcgcca ggtattcgaa caacttcatg
     1081 gggctggcgg tcgccgccgt atcgccgccg ctgctcaccg cgatccggcg ttcatacggc
     1141 atcagcaaca cttcgcaagc cgcttggacc agcggtagcc gctcattggg gacaaaccct
     1201 agcagcttca agttttcgat cccttctcgc tgcagccggg cctgccatcg gatcactgcc
     1261 tccggctcgc ctcccgccca cagccattgc acatcgggca tcgaggccgc caaggtcagc
     1321 atgagctcca agccccttcc cgcatacaga tgtccggtgt agccagcggt gaagcgatcc
     1381 gggagtccga gctccgcccg agcttggttg ggatctggca gtcctcggta gcgatccaga
     1441 tcgactccgt tcggcgagat cgcggccagc ggatcgatcc gctccgagcc atagtgtgcg
     1501 gccagccagc ctcgcagcgc ctcggtggtc acgagcgccc ggcgggcgcc ccttccccgg
     1561 aggaatgtgg caaacagccg gggacccatc cgaccggccg ggcgatcgtg gacctcgagc
     1621 acggtcggaa caccccaggc cgacccgatc gcggcggctt ggtacggcca aacgtagagc
     1681 agctcggctc ccgagcggcg cccggcgagc acggcccgaa ggcagaagtc gtagtggcgc
     1741 agtgcccgca aggaacgcag tcttcgaatt gggaatcgga cctggatccc atagtgggcg
     1801 gcgagctctt cccacccggg ttccggtcgc accgcgggca gccacagcgt cacctggtgt
     1861 ccgagctcga ccagcgccgc gcaggatttc atcagctgga tcgagtttgc ggtcgtggag
     1921 gggacttcgg aggtcgagat gcaggcgatg cgcaacttag gcaggtgaca aggcggcctt
     1981 tcgctggatt tcgcgcagag tcttccacac catcagtgcg gccgtgccga tgaagaaact
     2041 gcccaggcta atggccatcc ccaccgcccc caggcgggga accagcagga agatcagcac
     2101 aaccttggcc agagctgcag ccagggtcac cttggtcggg tattccggca agccaagcgg
     2161 gagcagcacg ctccgattcc agtagaagac actgacgacg atcactcccg gaagcagcac
     2221 cagcaaggcc aagtagcttt ccggcaggaa ctccgacccc cacagggaga ccacccagac
     2281 tccgaagatt gccagcccgc cggccgccgc cagcgagtag gctgcggcga tcagactgcc
     2341 gcttcttagt aagtaccgca cgttttccca gcgccgggtg gcgacctccc gggccacctc
     2401 ccgataggtg gtgctgatca gcggctggac cgggatgagc acaatgttca ggatcgccag
     2461 agccaccttg taatacccga cctgcagcgg cgaggtcaag gctcccagcc acaggacctc
     2521 gctgtcacgg gtcacgaggg tgagggtccc ggtcaggttg gtgctcagtg cgaagcgggc
     2581 catctctcgc cggcgatcga agatggaaga cagcggagcc cgccaccacc ctgggcccca
     2641 ccgccggcgg gccgcgatcc aggccgctag ggtaatggcg atcgcccagg ccacttttcc
     2701 gaccagatag gcccacacga ccgcgttcag ccctgctttc gccagcaccg cggcgaggat
     2761 cagtgccaga gtcagcccgc tctggccaac ctggatccaa gcctgcagac ggaactcgtc
     2821 taaatactgt aggactccgg cgctggtctc gaccaccagg tgggccagca ccatggaccc
     2881 gtagagtgtg aacaagccga caatgctcgg atctttggcc aggtagcgcg cacctacggg
     2941 ggccagcagc gcgatcagcc caaatgccag caccgagctg atcgcttcgg ccgctgccgc
     3001 agccttgacg gttgcggccg cccgggccgg ctcgtccctc gaagagaact cgccgacgta
     3061 gttcaccacc agttcgctca ttcggaagga ggtcagccga ttgattacgc tggcaaagac
     3121 ggtgatgatc cccaataccc cgaaagcctg cacacccagc agccgagccg ccaggattcc
     3181 ctgcagcatg ctcatcgcgg ccgaaaggcc gctgccggta aacaggtagc ccgagttgcg
     3241 catcacccgc tgcatgagcg ggttgcgcag catgcggccg agcgga
//
LOCUS       DATDPQ010000022         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3409026, whole genome shotgun sequence.
ACCESSION   DATDPQ010000022 DATDPQ010000000
VERSION     DATDPQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3409026"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VI520_00500"
     CDS             complement(<1..995)
                     /locus_tag="VI520_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HLF36390.1"
                     /translation="MTRQELRIWLLGPPRVELGGAALVVDTRKATALLIYLAVTGQEH
                     SRESLAVMFWPESDTGHAKAALRRTLSSLRKALNGEWLVIARDYLAVEPAWLDTQAFA
                     ELLAATLNHGHPAAESCTRCIAPLTQALELYRGDFLAGFSLPDSPQFDEWQYFTERAL
                     QRKCTEALQLLSAAHQAAGSYEKALEAADRLFAFDPLNETAHRQLMRLYVSLGRRDLA
                     LKQYRDCVRLLQTELGTPPLEETVDLYERIRAGEMTEPLRRQAPISAAAAPRPIPLVG
                     REPELRELRAWYRSVERSCKLIIVAGEAGVGKSRIGEEFLSGVTELGGKVLQARC"
     gene            1103..1597
                     /locus_tag="VI520_00505"
     CDS             1103..1597
                     /locus_tag="VI520_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36391.1"
                     /translation="MYSLLRFLHVFSAFNFFLIHGATAAAMFALKRERDPNRVKGLLA
                     IRDFATKWMGIPLAVLFISGIVMGFMGRWWNQAWIWLAIGVFLAISFLMSGFGRPYHD
                     RLWHALDPQGHAPPTKKEQRRTVPATTEELLGLLAKGRPMLLTVTGVIGLGVILWLMM
                     FKPF"
     gene            1676..2149
                     /locus_tag="VI520_00510"
     CDS             1676..2149
                     /locus_tag="VI520_00510"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HLF36392.1"
                     /translation="MYDLIRFVHVLTALIYFLFHGAIASVSFALRRAGTPERTEALEQ
                     VMRLVYHPIPTSLVIMVLSGLALGFLGSWWSDRWIWVSIGLLLFVGVLMNRLGKASLT
                     EGFAAGREPRSDTNPTEARPGSNPPRAFFLHPTFLTVTGVVALAVITWLMMFKPG"
     gene            2165..2653
                     /locus_tag="VI520_00515"
     CDS             2165..2653
                     /locus_tag="VI520_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36393.1"
                     /translation="MHKWLVFLHVLGAMGFMLAHGGTTAMTLLLKRQRGLDAMRALLD
                     LSRHSGPAMGIALLVLLGGGIAAGFTGRWWGEAWIWISLAILIGITAVMAWSSRAQYH
                     KLRKVLGMPYFDGRREQPAPPPAAEAEIYAVQATLRPGLLSVVSFGGIVLIAWLMMYK
                     PF"
     gene            2747..3169
                     /locus_tag="VI520_00520"
     CDS             2747..3169
                     /locus_tag="VI520_00520"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HLF36394.1"
                     /translation="MRRVARTIYVGIAWLALAGFLVNIYLAGMAVFVDRSYWDIHREF
                     GYAVSLIVPIFILLGLLSWIPRQLTAWLAGLLLYYLFHTILPVLQENLGWISAFHPVS
                     AVLLVWLGLVHARRAGALLLERRTAPTEPVPVKTASQV"
     gene            3185..>3787
                     /locus_tag="VI520_00525"
     CDS             3185..>3787
                     /locus_tag="VI520_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF36395.1"
                     /translation="MRRSVKYLILLAVSLIVLLAMGVFFVNRSVEQRLARTYDIPIEA
                     IEIPSDAAAVGRGQHLVGTIFFCQECHGEDLAGKPHFSDPLSGTVSAKNLTAGVGGVG
                     STFSDVDWLRAIRHGVDRDGHPLVEMPSNSYYYIGDVDLAAIIAYLKSLPPVDNELPE
                     RDLGLLYQLAILSDPSLIPAEVIDHTGPRPPAPEPGVNAEY"
BASE COUNT          660 a         1168 c         1158 g          801 t
ORIGIN      
        1 taacatctcg cctgcagaac ctttccacct agctccgtga cgccgctcaa gaattcttcg
       61 ccgatgcggg atttccccac tccggcctcg ccggccacta ttatcaactt gcagcttcgc
      121 tcgaccgaac ggtaccaggc ccgcagttcg cgcagttcgg gttcccggcc cacgagcggg
      181 attgggcgcg gtgcggcggc agcggagatg ggtgcctgcc tgcgcagcgg ctcggtcatt
      241 tccccggccc ggatgcgttc atagaggtcg accgtctctt ccaagggtgg cgtgcccagt
      301 tcagtttgca gcagtcgaac gcagtcccga tattgtttca acgcaaggtc gcgccggcca
      361 agactcacgt agagccgcat gagctgccgg tgggcggtct cgttcaatgg atcgaaggca
      421 aagaggcggt cggctgcctc aagcgccttc tcatagcttc ccgcggcttg atgggccgcc
      481 gacagaagct gaagggcctc cgtacacttt ctctgcagcg ctcgctcggt aaagtactgc
      541 cactcatcga actgtggact atccggcaag ctgaatcccg ccaggaagtc cccccggtag
      601 agctcgagtg cctgagtgag cggagcaata cagcgggtac agctctcggc agccgggtgg
      661 ccgtgattga gagtggccgc gagcagctcg gcgaaggcct gggtatccaa ccaggccggc
      721 tcgactgcca aataatcgcg ggcaatcacc agccattctc cgttcagagc cttgcgcagc
      781 gaggaaaggg tccggcgcag tgcggccttt gcgtggccgg tatcactctc cggccagaac
      841 atgactgcca ggctctcgcg gctgtgctcc tgcccagtta ccgccaggta gatcaacaag
      901 gcggtcgcct tacgggtgtc gacgaccaaa gctgctccac ccagttccac ccggggcggt
      961 cccagcagcc aaatcctcag ctcttgtctc gtcacaattg aattctaagg ccatcccgct
     1021 acgaaatcac gttcacttgt atatccgtaa cggttcttgg acggtccacg ggtattctgg
     1081 ccaataagaa agggcaaccg agatgtatag ccttctccgc ttcctgcatg ttttcagcgc
     1141 cttcaatttc ttcctgattc acggtgccac cgccgctgcg atgttcgccc tcaagcgtga
     1201 gcgggacccc aatcgggtta agggattgct cgccattcgt gacttcgcca cgaagtggat
     1261 gggaattccg cttgcggtct tgttcatcag tgggattgtg atggggttta tggggcgctg
     1321 gtggaaccag gcctggatct ggctggcaat cggggtattc ttggcgatca gcttcctgat
     1381 gtccggattc ggccggccgt accacgatcg gctgtggcac gcgctcgatc ctcaggggca
     1441 tgccccgccc actaagaaag agcagcgccg cactgttcca gccacgaccg aagagctgct
     1501 cggcttgctt gcaaagggcc gcccgatgct cctgaccgtg acgggcgtga tcggcctcgg
     1561 cgtcatcctg tggctgatga tgttcaagcc gttctgaggt tggtttgact ggcgctaggt
     1621 cactcggagc ttaagatgag ccggcgagtg cggacagact gatgtccggg gaacaatgta
     1681 cgacctaatt cgcttcgtgc acgtcttgac cgcattgatc tatttcctgt ttcacggcgc
     1741 gatcgccagc gtgtcctttg cgctgaggcg ggcgggcacc ccggagcgaa ccgaggccct
     1801 cgaacaagta atgcgattgg tttaccaccc gatcccaacc tcgctcgtca taatggtgct
     1861 gtcgggtctc gccctgggct tcctgggcag ttggtggagc gatcgctgga tttgggtgtc
     1921 gatcgggttg ctgctgttcg ttggggtctt gatgaaccgg ctcgggaagg cctccctcac
     1981 cgaggggttt gccgcaggca gggaaccgcg cagcgacacg aacccgaccg aagcgcgtcc
     2041 gggttcgaac cccccacgcg ccttcttcct gcatccaaca tttctcacag tgaccggcgt
     2101 ggttgcgctg gcggtcatca cgtggctgat gatgttcaag ccgggctgag ccgagcttta
     2161 cgcgatgcac aagtggctgg tcttcctgca tgtactcggt gccatgggtt tcatgctcgc
     2221 ccacggcggg acgaccgcca tgaccctgct gctgaagcga caacgcggcc tcgatgccat
     2281 gcgggccctg ctcgatttat cccggcatag tgggccggcg atggggatcg cgctgctggt
     2341 cctgctcggg ggcggaatcg cagccggttt taccggacgc tggtggggcg aggcctggat
     2401 ctggatctcg ctggcgatct tgatcgggat cacggcagtc atggcctggt ccagccgcgc
     2461 ccagtaccac aagctgcgta aggtgctcgg aatgccgtac ttcgacggtc gcagagagca
     2521 acccgcgccc ccgcccgccg ctgaagcaga gatttacgcg gtccaggcca cgctgcggcc
     2581 cggactgctg tcagtggtga gcttcggcgg gatcgtgctg atcgcctggc tgatgatgta
     2641 caagccgttt tgaaaccacc ggcttgctgc gcaataggcc tgaccatggg tgggtttaga
     2701 atgcgaggga gcgtgccgtg ccggcccgcc ccggaggaga agaaccatgc gtagggttgc
     2761 ccgcactatc tacgttggca ttgcctggct tgcgctggcc ggcttcttgg tgaacatcta
     2821 cctggccggg atggcggtgt tcgtcgaccg gagctattgg gacatccatc gggaattcgg
     2881 atacgccgta agcttgatcg tccccatctt catcctgttg ggcctgctgt cgtggatccc
     2941 tcgccagctc acggcctggc tggcaggctt gcttctgtac tacctcttcc acaccatact
     3001 tcctgtgctg caggaaaacc tgggctggat ttccgccttc catccggtta gcgcagtcct
     3061 gctggtctgg ctcgggctcg tgcacgcccg ccgcgccggc gcgctgcttc ttgagcgccg
     3121 cactgccccg accgagcccg tccccgtgaa aacggcctcg caagtgtaga gccggggctc
     3181 agctatgcga cgctcggtca aataccttat cctgctggcc gtcagcttga ttgtgctcct
     3241 ggcaatggga gtgttcttcg tcaaccgaag cgtagagcag cggctggctc gaacttatga
     3301 cattcccatt gaggcgatcg agatacccag cgatgcggct gcagtcgggc ggggccagca
     3361 cctggtcggc acaattttct tctgtcagga gtgccacggc gaggatttgg caggtaaacc
     3421 acactttagc gacccgctga gcggaacagt atcggccaag aacctgactg ccggcgtcgg
     3481 aggcgtgggc agcacgttca gcgatgtcga ctggttgcgg gccatccgcc acggagtgga
     3541 tcgggacggt catccactcg tcgaaatgcc ttccaactcc tattactaca tcggagacgt
     3601 cgacctggcg gccattatcg cctacttgaa aagccttcct ccagtcgaca acgagctgcc
     3661 ggagcgggac ctgggtctgt tataccaact cgccatcctg agtgacccat ctcttattcc
     3721 ggccgaagtg atcgaccaca caggtcctcg tccaccggca cctgagcccg gcgtcaatgc
     3781 cgagtac
//
LOCUS       DATDPQ010000023         2940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1577776, whole genome shotgun sequence.
ACCESSION   DATDPQ010000023 DATDPQ010000000
VERSION     DATDPQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577776"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VI520_00530"
     CDS             complement(<1..761)
                     /locus_tag="VI520_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLF36396.1"
                     /translation="MNNGRTFRPSLVSWNLTKRCNLRCPHCYMEAGVKAERELSTSEC
                     LSLIDEMGALGTEMLILTGGEPLLRKDIYDIASYASRRGIWVVMGTNGVLVNDLVAQK
                     MIECGVRGVGISIDSTDPAVHNSFRGGPNSWEQSVRALEICRGHGLEVLVQSTVMDLN
                     YAEVPRLLDFAKGLGAWSFNLYFLVQTGRGQQLNDLGAERTEQMLAFLAESQQRYRPM
                     LVRAKCAPHFKRIAFEAGLGGIESGGCMAGTEYCR"
     gene            complement(758..1168)
                     /locus_tag="VI520_00535"
     CDS             complement(758..1168)
                     /locus_tag="VI520_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36397.1"
                     /translation="MSGLAGDPHRQAAVGYGGIGAIVILITLFARLVPESRVNAVVEL
                     SIGLVFLMLFAILIYRGWWLVSAALVFSNAWRAFTYFNDGRGLHVELLARVITPIQPQ
                     PIAFLNAALMALIVLLLARSAVAGFSAWRAGRAA"
     gene            complement(1165..1575)
                     /locus_tag="VI520_00540"
     CDS             complement(1165..1575)
                     /locus_tag="VI520_00540"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HLF36398.1"
                     /translation="MEFVSNDTDRESGVMAIQYSCPECGHRISMVTNPGETQMVSSLG
                     VTIGHESLSEGAAGPMAQIRRSLDGDPAWTDDRGSPDPVWTEAAEKRLAAAPVFVQGM
                     VRRLYTDWAKRNGYGEVSPAVMSKARDDLGMSEM"
     gene            complement(1605..2477)
                     /locus_tag="VI520_00545"
     CDS             complement(1605..2477)
                     /locus_tag="VI520_00545"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HLF36399.1"
                     /translation="MEGTPAAGWIRELERPPVVDVLGQVVAQRAAVARRLASVRRVVA
                     IASGKGGVGKSLLTANLAAALTSNSGQVGVLDADIHGPSAALMLGARGQELMLGPEGA
                     RPALGVNGVQVISMDLFLEGDDRAVRWRNYGGLAEDSFVWRGTLEANVLREFLADTDW
                     GVLDYLLIDMPPGADRFETLLRLLPDLGGALLITTPSEASVLVVRRAAVAAQQAGARI
                     LGLVENMAQAADPRPGQDLADELGIPFLGRVPFDPELGRSTDTGRPGVLIDPERPGAR
                     AILELAGRLRGILA"
     gene            complement(2482..>2940)
                     /locus_tag="VI520_00550"
     CDS             complement(2482..>2940)
                     /locus_tag="VI520_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HLF36400.1"
                     /translation="RAFPWTEAALQRVQSRVPPFIQPMAMLAMERYAREKQLPEVTLD
                     VTIQVARRLGYAGDDGPGSELVWNEAALERLSRVPKFERQMVKEMVDSLAREAGHQEV
                     NYEIADEMLRRVRELWEAEGEGAFGYFKGMAGPGSADQSQGTRQPPGSNE"
BASE COUNT          536 a         1047 c          861 g          496 t
ORIGIN      
        1 atccggcagt attcggtccc tgccatgcaa ccgccgcttt cgatcccgcc tagtccggcc
       61 tcgaaggcga tccgcttgaa gtggggagcg cacttggccc gcaccaacat cggccggtag
      121 cgctgttggg actccgccaa gaacgccagc atctgctcgg tccgctccgc gccgagatcg
      181 ttcagctgct ggccgcgtcc ggtctgcacc aggaagtaaa gattgaaaga ccaagccccc
      241 agccccttcg cgaagtccag cagccggggg acctccgcgt aattgaggtc catcacggtg
      301 ctctggacca gcacctccag cccgtgaccc cgacaaatct ccagtgcccg taccgattgc
      361 tcccaggagt ttgggccgcc ccggaagctg ttgtgcaccg ccggatcggt cgaatcgata
      421 ctgatcccga ccccgcgcac cccgcactcg atcatcttct gcgcgaccaa atcgttcacc
      481 aggacaccat tggtgcccat taccacccag atcccgcgcc gggaggcgta gctggcgata
      541 tcatagatgt ccttgcgcag cagcggttcg ccgccggtca ggatcagcat ctctgtcccc
      601 agcgcgccca tttcgtcgat caggctgagg cattcggagg tgcttagttc gcgctcggct
      661 ttgaccccgg cctccatgta gcagtggggg caacgcaggt tgcagcgctt ggtcaggttc
      721 cacgaaacca aggacggccg aaacgtgcgt ccgttgttca tgctgcccgt cccgcgcgcc
      781 aagccgagaa tccggcaaca gcggaacgcg ccagcaagag gacgatcagg gccatcaggg
      841 ccgcgttaag gaaggcgatc ggttggggct ggatcggggt gatcacccgg gccagcagct
      901 caacgtgcag cccgcggccg tcattgaagt aggtgaacgc cctccaggcg ttggaaaaca
      961 ccagggccgc ggaaaccagc caccagccgc ggtaaatgag gattgcgaac agcatgagaa
     1021 ataccagccc aatcgacagt tccaccacgg cgttcactcg actctccggc accaatctgg
     1081 cgaagagagt gatcaggata acgattgctc cgattccccc gtacccgacg gcggcttgcc
     1141 ggtgggggtc tcccgccagc ccactcacat ctcggacatc ccgaggtcgt cgcgggcctt
     1201 gctcattacg gccgggctga cctcgccgta gccatttcgc ttggcccaat cggtgtacag
     1261 tcggcgaacc atgccctgga caaagaccgg agccgcagcc aggcgcttct cggccgcctc
     1321 ggtccagacc gggtccggcg atccccggtc gtcggtccag gcggggtctc cgtctagcga
     1381 gcgccgaatc tgagccatcg gccccgcagc gccctcggaa agcgactcgt gcccgatggt
     1441 gacccccagg ctggacacca tctgagtctc gcccggattg gtgaccattg agattcggtg
     1501 gccgcactcg gggcaggaat actgaatggc catcaccccg ctctcccggt cggtgtcatt
     1561 cgacacgaac tccatcgtcg cctgacattc ctcgcagcgg aatttcacgc caggattccc
     1621 ctcaaccggc cggccagttc aagaatggcc cgggcccccg gccgctccgg gtcgatcagc
     1681 actccaggtc gacccgtatc ggtgctccgg ccgagctcgg gatcgaacgg aaccctcccc
     1741 aggaagggga tcccaagctc gtcggccagg tcctgaccgg gcctcggatc ggccgcctgg
     1801 gccatgttct ccaccagacc gaggatccga gccccggcct gttgagcggc gacggcggcg
     1861 cgccgaacga cgagcacgga ggcttcactc ggggtggtta tcagcagcgc gcccccgaga
     1921 tcagggagca atctcaggag ggtctcgaac cggtcggcac ctggcggcat gtcaatcagc
     1981 agatagtcca gcactcccca atcggtgtcg gccaggaact cccgcaggac gttggcttcc
     2041 aaggtgcccc gccacacgaa agaatcctcg gcgagtcctc catagttccg ccagcggaca
     2101 gccctgtcat cgccttccaa gaacaaatcc atcgaaatca cctgcacgcc attgaccccg
     2161 agggccggcc gggcaccttc cggtccgagc attagctcct gcccgcgggc acccagcatc
     2221 agcgcggcgc tcgggccgtg gatatcggcg tccaagaccc cgacctggcc agaattgctc
     2281 gtcagagcgg ctgccaggtt ggcagtcagt agactcttgc caaccccacc ctttccgctg
     2341 gcgatggcca caacccgacg aacagaggca agccggcggg cgaccgccgc ccgctgagcg
     2401 accacctgcc ccaacacatc aaccaccgga ggtcgctcca actctctgat ccaccccgct
     2461 gcgggggtcc cttccatttc ctcactcatt cgagcctggt ggttggcgag tgccctggct
     2521 ctggtccgcg gagcccggtc ccgccatgcc cttgaaatag ccgaacgccc cctcgccctc
     2581 ggcctcccag agctcgcgca ccctgcgcag catttcatcg gcgatttcgt aattgacttc
     2641 ctggtggccg gcttcccgcg ccaggctgtc gaccatttct ttgaccatct ggcgctcaaa
     2701 cttgggaacc cgcgagagcc gctcaagggc cgcttcattc cagacgagct cgctccccgg
     2761 cccgtcgtca ccggcatagc ccaaccggcg cgccacttga atggtcacgt cgagggtcac
     2821 ctcggggagc tgcttctctc gggcataacg ttccatggcc agcatagcca tcggctggat
     2881 gaacggcggc acccgactct gcacccgttg gagggccgcc tcggtccagg ggaatgcccg
//
LOCUS       DATDPQ010000024         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_531612, whole genome shotgun sequence.
ACCESSION   DATDPQ010000024 DATDPQ010000000
VERSION     DATDPQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531612"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VI520_00555"
     CDS             <1..726
                     /locus_tag="VI520_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36401.1"
                     /translation="FEVFLPMLAVSAYVFIYRAIGAPEAYVGFVVMGGAMTAFWMNVL
                     WGMASQFFWEKETGNLALYTIAPTSIMAILLGMALGGMTATLLRALAIVLLGILIFNV
                     QFSVASFGLLFLTFTLTMVALYGMGMMTASLFLLLNREAWHLANLAQEPVYLLSGFYF
                     PLRSFNFWVALGASIIPMSLGLDAMRQLVFPVGASLGFLSVPVELAVLTGLSAAFLIG
                     AHFLLSYMERLSIREGRLTDRTR"
     gene            763..1671
                     /locus_tag="VI520_00560"
     CDS             763..1671
                     /locus_tag="VI520_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36402.1"
                     /translation="MQGQATTAGEVGIRRAGASGALDALRSFRMAAWLGWQIESNWTD
                     PFLFAVYSVIKPVAGAAILVVMYAVITRGDFDAPLFSYILLGNAFYIYVGQIMSGISW
                     AVIEDREHYRTMKYLYIAPIRIPYYLIGRGAARFVTGSVSVAITLLAGVLFLHLRLVP
                     SEINWPMFLGAWLLGVVMLAMLGLLLAGVTMITANNVYVIGETVAGALYLFSGAIFPL
                     EVLPTWLRPIGFAIPLSYWLELLRRSLLGSVAQAFPTFAQLSDGQLFAILGSLTLVFG
                     LASVLAFRYCDFLARERGMIDRISNW"
     gene            1720..>3086
                     /locus_tag="VI520_00565"
     CDS             1720..>3086
                     /locus_tag="VI520_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HLF36403.1"
                     /translation="MLLDWALAALPVALLLVLMVGFRWGAAKAGPAGWLVATILAVLR
                     FGAGIQLMGVAHAKALLLALDVLFIIWGAFLLYRVADEAGAVEVISRFLPQLYKDQGM
                     QALLIGWPFASFLQGVGGFGVPVAVTAPLLIGLGFGPIPAMVIPSLGHAWSVTFGSLG
                     SSFQALIGSTGVPGEQLAPAAAGLLGVATFGCGILAAHAAGSRAAILRLAGPLLLIGA
                     AMAGVQYWLATHQLWHLAGFGGSLAGLLVGLFFARRPKGGQTLRPLGLALAAYLALIL
                     ITLAVQLIPPLEQGLGRVQIRFDFPEVQTELGFVTAAGPGRIIPIFRHAGALLGYASL
                     VGYLVYRRTGRYQAGALRRIVGGTVSRVAPASLAVLALVSMAVVMTHAGMTERLAGGL
                     AQAAGRAYPLLAAWIGGLGAFITGSNTNSNVIFGQLQLRTAQLSGYRVPVLMAAQTAG
                     GAIGSV"
BASE COUNT          440 a          911 c         1030 g          705 t
ORIGIN      
        1 ttcgaggtct tcctgccgat gctggcggtc tccgcctacg tctttatcta ccgggccatc
       61 ggtgcgccgg aggcctatgt cggcttcgtc gtcatgggag gagccatgac cgccttctgg
      121 atgaacgtgc tgtggggaat ggcgagccag ttcttctggg aaaaggaaac cggtaacctg
      181 gcgctgtata cgatcgcacc gacctcgatc atggcgatct tgctggggat ggcactcgga
      241 gggatgactg ccaccctgct gagggcgctg gcgatcgttt tgctcgggat cctgatcttc
      301 aacgtgcagt tcagcgtggc ttcgtttggg ctgctgtttc tgacctttac cctgacgatg
      361 gtcgccctct atgggatggg gatgatgacc gcttcgttgt tcctgctctt gaaccgggag
      421 gcctggcatc ttgcgaacct ggcccaggaa ccggtgtact tgctctcggg gttttacttc
      481 ccactgcgca gtttcaattt ctgggttgcg ttgggggcct cgatcattcc gatgtcgctc
      541 ggattggacg cgatgcgcca gctggttttt ccggtaggag ccagcttggg cttcctctcc
      601 gtgccggtgg aattggccgt gctgaccggg ctgagcgcgg ccttcttgat tggcgcccat
      661 tttctgttga gctacatgga acggctgtca atccgcgaag gacggctcac cgaccggacc
      721 cgctgaggcg gagtccggag attcggatcg ggcgatcagg ccatgcaagg gcaagcgaca
      781 acggcggggg aagtgggaat acgcagagca ggggcctccg gggcgcttga cgccctccga
      841 tcttttcgga tggcggcctg gctgggctgg caaatcgagt ccaactggac cgatcccttt
      901 ctcttcgcgg tctactcggt catcaagccg gtcgccgggg cggcaatcct ggtggtcatg
      961 tacgcggtca tcacccgagg tgactttgac gccccgctct tctcctacat cctgctgggc
     1021 aacgctttct acatttacgt aggccagatc atgagcggaa tctcgtgggc ggtgattgaa
     1081 gaccgcgaac actatcggac gatgaagtac ctgtacatcg cgccgattcg gattccctat
     1141 tacctgatcg gtcgcggggc ggcccgcttc gtgaccggat cggtctcggt ggctatcacc
     1201 ctcttggccg gggtcttgtt cctgcacctg cggctggtcc cgagcgaaat caactggccg
     1261 atgttcctgg gggcctggct gctgggggtc gtgatgctcg ccatgctggg gctgctgctt
     1321 gccggggtaa ccatgatcac ggctaataac gtgtacgtga tcggcgaaac cgtggccggg
     1381 gcactgtatc ttttcagcgg ggccatcttt ccattagaag tgctgccgac ctggctgcgc
     1441 ccgatcgggt tcgcgatccc gctttcctac tggttggagc tgctgcgccg ctcgctgctg
     1501 ggaagcgtgg cgcaggcgtt tccgaccttt gcccagctaa gcgacgggca actcttcgcc
     1561 attctgggga gcctgaccct cgtgtttggg ctggcttcgg tcttggcctt ccgttactgc
     1621 gacttcctgg ctcgggaacg cgggatgatc gaccggatca gcaactggtg acccgccgca
     1681 tcgggccgag cctcactccc cgaagtctca atcgaatcta tgctccttga ttgggcgctg
     1741 gctgcgctac cggtagccct gctcctggtc ttgatggtgg ggtttcgctg gggagcggcc
     1801 aaagccggac cggcaggatg gttggtggcg acgatcctgg cggtccttcg ttttggagcg
     1861 ggcattcagc tgatgggtgt cgcacatgcc aaagcgctgc tgctggctct ggatgtgctg
     1921 ttcatcatat ggggggcatt tctcctttac cgggtggccg acgaggccgg ggcagtcgag
     1981 gtgatcagtc gattcctgcc ccagctgtac aaggatcagg ggatgcaagc cttactcatc
     2041 ggatggccgt ttgcctcttt cctgcaaggg gtaggcggct ttggggttcc ggttgcggtc
     2101 accgcgccgc tgctgatcgg gctcggattc ggacccatcc cggctatggt cataccctcg
     2161 ctggggcacg cctggtcggt caccttcgga tcactcggct cgtcgttcca ggccctgatc
     2221 gggtcgaccg gcgtaccggg tgaacaactg gctcccgccg cagcagggct gctcggtgtg
     2281 gccacattcg gctgtgggat cctggccgcc catgccgccg gaagccgggc agcaatcctg
     2341 cggctggccg gtccgcttct cttgatcggg gcagcgatgg ccggcgtgca gtactggctc
     2401 gccactcacc agctttggca tctggccggg ttcggcggca gtctggcagg cctgctggtc
     2461 gggctgttct tcgctcggcg gccgaagggc ggccaaactc ttcgcccgct cggcttggcg
     2521 ttggcggcct acctggcgct gatcctgatc acgttggcgg tgcagttgat cccaccgctg
     2581 gaacagggcc tgggccgggt acagatccgc ttcgattttc cggaggtcca gacggaattg
     2641 gggtttgtca ccgcggccgg accgggccga atcatcccga tcttccggca tgccggcgcg
     2701 ctgctgggct atgcatcctt ggtgggttac ctcgtctatc gtcggaccgg gcgatatcag
     2761 gctggagccc tgaggcgaat tgttggtggc acagtctcgc gggtcgcccc agccagcctg
     2821 gcggtgctgg cgttggtatc gatggcggtg gtgatgaccc atgccggcat gaccgagcgc
     2881 ctggccgggg gactggcaca ggccgcgggg cgggcctatc cgttgctcgc agcctggatc
     2941 ggcggattgg gagccttcat caccggctcg aataccaact cgaacgtgat ctttgggcag
     3001 cttcagctgc gcactgcgca actatcgggt taccgagttc cggtcttgat ggcggctcaa
     3061 accgccggtg gggcgatcgg atcggt
//
LOCUS       DATDPQ010000025         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_793906, whole genome shotgun sequence.
ACCESSION   DATDPQ010000025 DATDPQ010000000
VERSION     DATDPQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793906"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2082)
                     /locus_tag="VI520_00570"
     CDS             complement(<1..2082)
                     /locus_tag="VI520_00570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF36404.1"
                     /translation="MEDLSESTPEPSVLDWLKSLFKLKPLPIPERAGAAPVVELRRVV
                     AQPATPASTQVDIRKWAGQLRLPVALLLAFLAQVGLEQHKNVQSNVLLYLLAGGLIGW
                     SLWTGELDRDAEKTKAERSWRTWIDLRYLSGGIILSGLTYLASGDNTFRFSTLVFWVL
                     AILATGRAFWQGKPPTARLTSGVREFLGKPRIRISIGASGLLALAAFGLAAGFRYARL
                     ADVPYEMTSDHAEKILDIVDVLNGQTSIFFPRNAGREAIQFYSTAAAVHWFGAELDFL
                     TLKRVTATAGLLALPFIYLFGREIGGRKLGLAAMALTGIAYWPNVIGRVGLRATFYPL
                     LLAPTLYFLVRGLRARRQNDFVLCGFFLGASIYSYTSARLAPLVVVLAVLLYVVHRES
                     KGHRLQALRWLAVTALMAVVVAVPFLRAAYQLPDQVFFRTVTRVTEMERALEGSVTGI
                     FFNNVLEALKMFSWDFGEIWVLSIPFRPALDWVTGGLFHLGVVLLFVRYAKSRNWLDL
                     FLPLSILVLILPSVLALAFPGENPHPSRAGGAIIPVFAIAATTLVAIEQWGTRKLSGK
                     TGQKIGAGFAGALFLLAAVGNYSWGVTLYGEHMRRGSWNASELGSIARGFGESVGSFD
                     QIYVMAYPHWLDSRLVAVDAGRPGQDFGIWPEQVSALEPTTEARLFFLHPMDENGHSL
                     LGGLFPNAILST"
     gene            complement(2089..4158)
                     /locus_tag="VI520_00575"
     CDS             complement(2089..4158)
                     /locus_tag="VI520_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF36405.1"
                     /translation="MRILTVLTYYSPHWTGLTAHAARVAEGLAARGEQVTVLTTRYRR
                     DLPRDETLNGVRVIRIQPVGRLSRGMITPAFPWAAALLIRAHDVVQIHTPLPEAPLVA
                     LVCRLLGRPLLMTHHGDLVMPPGAFNQALERLGYWMSYLAARLATAVTSYSLDYADHS
                     ELLGHFRNKLSAIYPPVEIPDPDRAAARAWRAELGLGDRAVIGFAGRWVSEKGFDDLL
                     RALPLVHRELPQAHLVFAGEQQVVYERFFEQCRPLIEPIRQHLTLLGLLHNRQQMANF
                     YAMCDLFALPSRTDMMALVQVEALLCGTPVVASDIPGARVVVAETGYGLLSPASDPQR
                     LADALVAAFRRRDSLRPNTVELRRIFDSQQSLTAYQELLSKLSGKPAQTRTPRRQPGF
                     HSANGSEPRDSLSAGDHQLLDRLLSNEADMAFRRRARMLLEYLELREGDRVIDVGCGM
                     GVYLMLMSRLRRMDLFGLDLQVERLRRARAEVTTARLVCGDIGRLPFAPGGFDKVLMS
                     EVLEHVPDDEGALRAIHRLLKPGGLLAVSVPHANYPFWWDPINRTWTRVGGSPIRKGP
                     LVGIWTDHQRLYLPDELEARLERAGFIVEAVEQATHYCFPFMHFLVYGVGKPLIEREL
                     LPRALANSADRFRAEQNQGGFLNPVNVGVAAFRAFDQANEGPEAADQGTYVNVLARAR
                     KPGGRQD"
     gene            complement(4264..>4669)
                     /locus_tag="VI520_00580"
     CDS             complement(4264..>4669)
                     /locus_tag="VI520_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLF36406.1"
                     /translation="DPRVVLMERKNARTLSELPEPIRLATVDVSFISLRLILPSVAGW
                     LVAGGEVVALVKPQFEAGRRQVGKGGVVKDPGTHQQVLFEILEFAIVAGLAPQGLIRS
                     PLVGPKGNVEFLAWLRKGGDPANLERLVSGTS"
BASE COUNT          945 a         1511 c         1453 g          760 t
ORIGIN      
        1 ggttgagaga atcgcgtttg gaaagagccc accgagcagg ctgtggccgt tttcgtccat
       61 cggatgcagg aagaatagac gagcctcggt cgtcggctca agcgcgctga cctgctctgg
      121 ccagataccg aagtcctggc ccggccgacc ggcgtccaca gccaccaatc gcgaatcgag
      181 ccagtggggg taggccatta catagatctg atcaaagctg ccgaccgact cgccgaagcc
      241 gcgggcgatc gagcccagct cggaggcgtt ccaagaacca cggcgcatat gctcgccgta
      301 gagcgtcact ccccatgaat agtttcctac cgcggcgagc aggaagagtg ccccggcgaa
      361 gccggccccg atcttctggc cggtcttccc gctgagtttt ctggtccccc actgctcgat
      421 cgcaaccagg gtggtcgcgg cgatcgcaaa gaccgggatg atggcgcccc cggcccgact
      481 cggatgcggg ttctcccctg ggaaggccag cgccaggacc gagggcagaa tcagcaccag
      541 tatagaaagc ggcaagaaca gatccagcca attgcgactc ttggcgtagc ggacgaagag
      601 caacacgaca cccagatgga aaagccctcc ggtcacccaa tccagcgccg gccggaacgg
      661 aatagacaat acccagattt cgccgaaatc ccaactaaac atcttcaggg cttccaatac
      721 gttgttgaaa aagatcccgg tcacagatcc ttccaaggct ctttccatct cggttacccg
      781 ggtcaccgtt cggaagaaga cctgatccgg cagctggtag gcggcgcgca agaaggggac
      841 ggcaacgacg accgccatca gcgcggtcac tgccaaccag cgcaaggcct ggaggcggtg
      901 tcccttggac tcgcgatgaa cgacataaag cagcacggcc aggacgacca ccaagggcgc
      961 cagtctggcc gaggtatagc tgtagatcga ggcccccagg aagaatccgc agagcacaaa
     1021 atcgttctgc cggcgggccc gcagcccgcg gaccagaaag taaagggtcg gtgcgagcag
     1081 cagcggatag aaggtagccc gcaggcccac tcgaccaatg acgttcggcc agtaggcgat
     1141 tccagtcaac gccatcgctg cgaggcccag cttccgccct ccgatctccc gtccgaagag
     1201 gtagatgaac ggcagggcga gcagaccggc agtggcggtg acccttttga gagtcaggaa
     1261 atcaagctcc gccccaaacc aatggactgc cgcggcggtg gaatagaact ggattgcctc
     1321 tcgcccggcg ttgcggggga aaaaaatcga ggtctggcca ttcaggacat cgacgatgtc
     1381 caagatcttc tccgcatggt cactcgtcat ctcgtagggg acgtccgcca gccgggcata
     1441 tcgaaacccc gccgccagac cgaacgcggc aagcgcgagc aggccggagg ccccgattga
     1501 aatccgaatg cgaggtttgc caaggaactc tctgacgccc gaggtgagcc gggctgtcgg
     1561 cggcttcccc tgccagaaag cccgcccggt cgccaggatc gcaagaaccc agaagaccag
     1621 ggttgaaaac cggaaggtgt tgtcgccgga tgccaggtag gtcaacccgg agagaatgat
     1681 cccgccgctc agatatcgca ggtcgatcca agtccgccag gaccgttccg ccttagtttt
     1741 ctcggcatcc cggtcgagct cacccgtcca gagcgaccac ccaatcaagc cgccggcgag
     1801 caggtagagc agcacattgg actggacgtt tttgtgttgc tccagaccca cctgcgcgag
     1861 gaaggccagt aggagcgcca ccggcagccg cagctggccg gcccactttc ggatgtcgac
     1921 ctgggtggaa gctggcgttg ccggctgggc gaccactcgc cgcagctcga cgaccggcgc
     1981 agccccggct cgttctggga tggggagcgg cttcagcttg aagagcgact tgagccagtc
     2041 caggacggat ggttcagggg tcgattcaga tagatcttcc attgaggatc agtcctgccg
     2101 gccacccggc ttgcgagctc tcgccaagac attgacatat gtgccttggt cggcggcctc
     2161 ggggccctcg tttgcctggt cgaacgcccg aaaagccgcc accccgacgt ttaccggatt
     2221 caaaaagcct ccctggttct gttctgcacg aaatcggtcg gccgagttgg cgagggctct
     2281 cggcagcagt tcgcgctcga tcaacggttt cccgaccccg tacaccaaga agtgcatgaa
     2341 ggggaagcag taatgggttg cctgctcgac tgcctccacg atgaaaccgg ctctttccaa
     2401 tcgggcttcc aactcatcgg gcaagtaaag ccgctggtga tcggtccaga ttcccaccag
     2461 cggacccttt cggatcggac tcccaccgac tcgggtccag gtccggttga tcggatccca
     2521 ccagaacggg tagtttgcgt gtggcacgga gaccgccaac agaccacccg gcttgagcag
     2581 ccggtggatg gcccgcagcg ccccctcatc gtccgggacg tgttcaagga cctcgctcat
     2641 caagaccttg tcgaaccccc ccggcgcgaa cggcagccgc ccgatgtcgc cgcaaaccaa
     2701 tcgggctgtc gtgacctcgg cccgggccct gcgcaggcgc tccacctgca gatccagccc
     2761 aaacaggtcc atgcgacgca ggcgggacat tagcatcaag tacacgccca ttccgcagcc
     2821 gacgtcaatg acccgatcgc cctcccgtag ctccaggtac tccagcaaca ttcgggcccg
     2881 gcggcggaag gccatgtcgg cttcgttgga gagtagtcgg tccagcagct gatgatcgcc
     2941 ggctgagagc gaatcccgag gctcggagcc gttggctgag tggaacccag gctgccgcct
     3001 gggggtccgg gtctgggctg gcttcccgct caacttcgag agcaactcct ggtaggctgt
     3061 cagggactgt tgggaatcaa agatccgccg cagttccacc gtattcggcc gcaggctgtc
     3121 gcgccgccgg aatgcagcga ccagagcgtc ggcaagccgc tgcgggtcgc tggcggggga
     3181 gagcagtccg tatccggtct cggccacgac cacccgggcg cccgggatgt cgctggcgac
     3241 caccggcgtg ccgcacaata gggcttccac ctggaccaag gccatcatgt cggtgcggct
     3301 gggcaacgca aacaggtcgc acatggcgta gaagttcgcc atctgctggc ggttgtgcag
     3361 cagcccaagt aaggtgaggt gttgccgaat cggttcgatc agcggccggc actgctcgaa
     3421 gaagcgctcg taaaccacct gctgttcccc ggcgaagacc aaatgggcct ggggtagctc
     3481 acggtggact agcggaaggg cccgcagcaa gtcgtcgaac cctttctcgg agacccaacg
     3541 gccggcaaat ccaatcacgg cccggtcgcc caggcctaat tcggccctcc aggcccgagc
     3601 cgctgcccga tccggatccg ggatctccac cggcggatat atcgcgctca gcttgtttcg
     3661 aaagtgaccg agcagttctg agtgatccgc atagtccaga ctgtagctgg tcaccgcagt
     3721 cgccaaacgg gccgccagat acgacatcca gtaccccagc cgctccaggg cctgattgaa
     3781 ggctccgggc ggcatgacca gatcgccgtg gtgggtcatc agcagcggac ggcccagcag
     3841 gcggcacacc agcgcgacca gcggggcctc cggcagcgga gtgtggattt gcaccacatc
     3901 atgggctcgg atcagcaggg ccgccgccca ggggaaggcc ggagtgatca ttccccggct
     3961 aagccggccg accggttgga tccggatcac ccggacaccg ttcagggtct cgtcccgggg
     4021 gagatcccgc cggtatcgtg tggtaaggac ggtgacctgt tcgcccctgg cggccagtcc
     4081 ttcggccact cgggcggcgt gagcggtcag cccggtccag tgcggggagt agtaggtgag
     4141 aacagtcagg attctcatcg cgggggaagc ttggccattc cggggctaga gcattccaag
     4201 tcgacgatta tagcgatccg ccctggggtt gacaattcgg acccgtttcc cacccggcgg
     4261 agcctaggaa gtgccgctga ccagcctttc gagattggcc ggatcgcccc ccttgcgcag
     4321 ccaggccagg aactcgacgt tccccttggg tccgaccagg ggcgagcgga tcaacccttg
     4381 aggagccaac cccgccacga ttgcgaattc aaggatctcg aaaagaacct gctggtgtgt
     4441 tccgggatcc ttgaccacgc cccccttccc gacctggcgc cttcccgcct cgaattgagg
     4501 cttgaccagc gcgacgacct cgccgcccgc gaccagccag cccgccaccg aaggcaggat
     4561 gaggcgcagc gagatgaaag acacatcgac cgtcgccagc cgaatcggct cggggagttc
     4621 gctcaaggtt ctggcgttct tgcgttccat gagcaccacc cgaggatcg
//
LOCUS       DATDPQ010000026         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1318875, whole genome shotgun sequence.
ACCESSION   DATDPQ010000026 DATDPQ010000000
VERSION     DATDPQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318875"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VI520_00585"
     CDS             <1..1396
                     /locus_tag="VI520_00585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLF36407.1"
                     /translation="WLEWDHPQMPWDGTELWLADLPSGISLGNPRRVAGGPTESIFQP
                     EWSPTGVLHFVSDRTGWWNLYRWADDRVDALLPMEAEFGSPAWQFGYSHYAFLPDGRI
                     VAVYIANGIDHLGWFPAEGQEWSPIETGLTSFDPPSLHFDGSSGRLVLVGGAPDRPPA
                     VHALQLDSGELESLSAIDPVLPDPGYISRPRPITFPTSGGQVAHALFYPPQNRDHQAP
                     ANTLPPLLVISHGGPTSNATSEFYLPLQFWTSRGFAVVDVNYRGSSGYGRAYRELLNG
                     TWGVFDVEDCIAAANYLVVQGEADPERLIIRGGSAGGYTTLCALTFHDEFAAGASYYG
                     VADLETLARDTHKFESRYLDSLIGPYPQAIETYRQRSPIHFAGQLACPIILFQGLEDR
                     VVPPAQAEIMVEALQSKGLPHAYLAFEGEGHGFRKEENIRRALEAELFFYSRIFDFDL
                     ADKLAPVEIKNLPG"
     gene            complement(1411..2715)
                     /locus_tag="VI520_00590"
     CDS             complement(1411..2715)
                     /locus_tag="VI520_00590"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HLF36408.1"
                     /translation="MRAVDVISKKRDGGELTKEEIEFFVAGFVRGEIPDYQASAWAMA
                     VLLRGMNARETTDLTLAMVASGDRLDLSQVVPLAVDKHSTGGVGDKTTLVVEPVVAAC
                     GVPVGKMTGRGLGFSGGTLDKLESIPGYRADLTAEEFRDQLARVGVVLSGQSVDLAPA
                     DGKLYALRDVTGTVPSIPLIASSVMSKKIAAGSQAVVLDVKVGVGAFMQTLEQAEQLA
                     KLMVEIGHRAGLKVVALLSDMNQPLGNAVGNALEVKEALATLSGEGPPDFVEHCLEVA
                     GHMLRLAGRASDLAGARSIAQAALADGSALRKFRALVEAQGGDPRYVDEPERLPAAQL
                     VEQFRAPTAGYLSSIHAAEIGHAAVELGAGRSKQTDPIDHSVGVVVHRKVGDRVVEGD
                     PLFSVHANDSTRMRSAIERVRAAHQFSIHPVDPLPLFYGVIE"
BASE COUNT          498 a          957 c          805 g          507 t
ORIGIN      
        1 ctggctggaa tgggaccacc cgcaaatgcc ttgggatgga accgagcttt ggctagccga
       61 tctgccgtcc ggaatctcgc tcggaaatcc gcgcagagtt gccggcgggc caaccgaatc
      121 gatcttccag ccggagtgga gcccaaccgg agttttgcac ttcgtctcgg accgaaccgg
      181 atggtggaac ctgtaccgct gggccgatga tcgcgtcgat gcactccttc cgatggaggc
      241 cgagttcggc tctccggcat ggcaattcgg atactctcat tacgcgtttc ttcccgacgg
      301 ccggatcgta gcggtctaca ttgcaaatgg gatcgaccac ctgggctggt tcccggctga
      361 aggtcaagaa tggtccccga tcgagaccgg gctgaccagt ttcgacccgc caagcctcca
      421 cttcgacggc agttccggcc gactcgtgct ggtcggggga gcgcccgacc gacccccggc
      481 ggtgcacgca ctgcaacttg actctggcga gctcgaatct ctcagcgcaa tcgacccagt
      541 gctccccgac ccgggctaca tttcccgtcc ccgcccgatt accttcccca cctcgggcgg
      601 tcaggttgcg catgccttgt tctaccctcc ccagaaccgt gatcaccagg ccccggcgaa
      661 cactcttccc ccgctgctcg tgatcagcca tggggggcca accagcaacg ccacttccga
      721 gttctacctg cctctccagt tctggaccag ccgcggtttc gcggtcgtgg atgtgaacta
      781 ccgcggcagc tcgggatacg ggcgcgccta tcgagagtta ctgaatggaa cctggggagt
      841 gttcgacgtc gaggattgca tcgccgccgc caactacttg gtagtccaag gggaagccga
      901 cccggagcga ttgatcatcc gagggggaag cgcaggcggc tacacgaccc tctgcgccct
      961 gaccttccac gatgagttcg cggccggagc aagttactac ggagtggcgg atctggagac
     1021 gctcgcccgc gacactcaca aattcgagtc gcgctacctg gacagcttga tcggtcccta
     1081 tcctcaggcc atcgaaactt accggcagcg ctcacccatc cactttgcag gccaactcgc
     1141 ctgtccgatc attctcttcc agggactcga ggatcgagtc gtcccgccgg cgcaggccga
     1201 gatcatggtc gaggcgttgc agtccaaagg gctcccgcat gcctacctgg cgttcgaagg
     1261 cgagggccat ggattccgaa aagaagagaa tatccgccgg gccttggagg ccgagctctt
     1321 cttctactcc cggatcttcg actttgatct cgcagacaag ctagcaccgg tcgagatcaa
     1381 gaacttgcct ggctagctgc gtgcccgcgg ctactcgata acgccgtaga agagcggcag
     1441 cgggtccacc gggtggattg agaactggtg ggcggcgcgg acccgttcga tagctgatct
     1501 catccgcgtg gaatcgttgg cgtgcacgct gaacagcggg tcgccctcaa ccacccggtc
     1561 ccccactttg cgatgcacga caacccccac cgaatgatcg atggggtcgg tttgcttcga
     1621 cctgccggct cccagttcaa ccgccgcatg cccgatctcg gccgcgtgga tcgaactcag
     1681 gtagccggcg gtcggcgcac ggaattgctc gacgagttgg gccgcaggca gccgctccgg
     1741 ttcatccacg tagcgcgggt ctcccccctg ggcctcgacc agcgcccgga acttccgcag
     1801 cgccgacccg tcggctaggg ccgcctgcgc gatcgatctc gcccccgcca ggtccgaggc
     1861 ccggcctgcc agccgcagca tgtggcccgc gacctccagg cagtgctcga cgaagtcagg
     1921 cggcccttcc ccgctcaagg tggccagggc ttccttgact tcgagtgcat tgccgaccgc
     1981 gttgccgagc ggctgattca tatccgatag cagcgccacg accttcaggc ccgcccgatg
     2041 gccgatctca accatgagct tggcgagctg ctcggcctgc tcaagggtct gcatgaacgc
     2101 ccctaccccg actttgacgt ccaagacgac tgcctggctg ccggcggcga tcttcttgct
     2161 catcaccgag gaggcaatca gcggaatgga aggtaccgtg ccggtcacgt cgcgcagagc
     2221 gtagagtttg ccgtctgcag gcgctagatc gacggactgc ccgctgagaa cgaccccgac
     2281 ccgagcaagc tgatctcgga actcctcggc cgtcagatcc gcccggtaac ccgggatcga
     2341 ttccaacttg tccaaggtac cgccggaaaa tccgagcccg cggccggtca tcttcccaac
     2401 tggcactccg caggcggcca ccaccggttc gacaaccaaa gtcgtcttgt cgcccacccc
     2461 tccggtcgag tgcttgtcaa ccgccagcgg cacgacctgc gagaggtcca gccggtcgcc
     2521 cgaggcgacc atcgccaggg tcaggtcggt cgtctcgcgg gcgttcatcc ctcgcagcag
     2581 caccgccata gcccaggcgc tcgcctggta atcggggatc tcacctcgga cgaatccagc
     2641 cacgaagaac tcgatctctt ctttcgtgag ctctccgccg tcacgcttct tggaaatgac
     2701 gtcaaccgcc cgcatgacct gagcatacta taggctccac ccgactgcta taatccagcc
     2761 ccatgcc
//
LOCUS       DATDPQ010000027         5030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3674194, whole genome shotgun sequence.
ACCESSION   DATDPQ010000027 DATDPQ010000000
VERSION     DATDPQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3674194"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3046)
                     /locus_tag="VI520_00595"
     CDS             complement(<1..3046)
                     /locus_tag="VI520_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HLF36409.1"
                     /translation="MEIPQPPEEGSRAGQDLLNLLIPLVTIIGFVLVSGTGNVLFIIP
                     IGLAMVLTIGVTISGARRQRKELEAKKKAYLEKLAELRQDMNRAHNAQRIFYSHNYPE
                     IPTVYEIAARKETSRFGPRLWERRTDDRDFGVVRLGMGTRPSSVVYRLSQAGNPLDEG
                     PLAKDARKLAQDSEILTDVPITIPLRRFVADSVGGSEARNGNLGGSVEARHSIGVFGK
                     NPGNTADFVRALVAHYAAFQSATDARLYVIGHPQAQPSWQWAEWLPHCNIRDVGLDES
                     VESGPPARLDQLCFAAEKEQVTAFWDRLKRELDQRQLRMQETDEEERREGDISLPHLL
                     VVVDLTGDLPADSPLAEVASEALIATVNQAGPALGASVIFLANDPLKIPSDCQAMVEV
                     AAAGKKVVFRYAEVGVNSARYLGDADLASASDARQLLAAKIRRLDIRRPFGADLPRLV
                     DLLQMESRVEGRKLDTVDKLPIQDNWRRSIRPENSEWLSAPIGMTSMRDVRSLVISAK
                     EGGDGVHGMIAGTTGSGKSELLLTLIASLAAKYDPRIVNFVLVDFKGGAAFEPFRRLP
                     HCVDILTNLEAHAVERMFVAIQAVMDERSRILAKSGAKDLVEYRKKVIPRLGADDSLP
                     RTFPHLFIIVDEFAEMIAANPEYRNQFESITRLGRAFGVTLILSTQRPAGVVTDQMRA
                     NMKFRICLRVETPEDSKELLGRPDAAFLPNIGGRGYIQVGNDILVPVHVARVAGDYSD
                     DRTVTLRDVIWLDEQSGPGEQAGEDQPLYSALEVAEALGLEAGEYPSTMLDWIVGITV
                     IRAERDGVPTQTKPWPEPLPPSLSLTDPVDARYLNTERALSADRTIVISEQLDGWIRN
                     SQEKQIWPTFDWRNPRPLQTTLGLIDNPYRAEQRLMSLDLADGPIGIFGAAGRGKTTF
                     VKTLLLGLAATRTPNDLHVYALDFGRAGLKSIQDLPHLGASIDAAEVTRVDQLMRMLR
                     NFLNERQEVLTKYNSLADYNSKNPKELFPEIVVVIDNFAEFNE"
     gene            3300..4427
                     /locus_tag="VI520_00600"
     CDS             3300..4427
                     /locus_tag="VI520_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36410.1"
                     /translation="MNRFCERCERVTVDGNLWCQDPDCPAEEGYELLGYGDFLGDLKV
                     TKLVRVWRSAAVYEAERGKLPVLLKVAHPTDACVERLKREATALHSLSPAQSGPLAFV
                     RSFLPSSRPALPRILPPYPSKLKRPFGEITFRGEPRVYSVYQHARGKILSDVLLENPQ
                     VWHSQAAWVISAVAEALRPLVAGNRCHLCLTPDIILIDTDRHGNLRPMLLDLGLILEP
                     GPNGANQDRNKLSEPAYTAPELLANSHDEIPTLSADVYSLGLIYFEMLAGRPGFESKL
                     RRDDQLREDVTENRKPLSIDRPELDQSGVTAIVERAVGSTGRYNNVIELAAALTKIYS
                     NPPPERRPIPRRLYFLIAFLGTFLLIAAVFAAATLLQVVAR"
     gene            complement(4482..4796)
                     /locus_tag="VI520_00605"
     CDS             complement(4482..4796)
                     /locus_tag="VI520_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36411.1"
                     /translation="MATTTVQMDYKVISDVSKGFKTAADVLTTVAKVLEVLINILRAM
                     AFASMGTTLALANYLEQIKNKIKPLAKLCTEFSGDLASAVGDHKKGDIAGKRYFGEGV
                     RR"
     gene            complement(4823..>5030)
                     /locus_tag="VI520_00610"
     CDS             complement(4823..>5030)
                     /locus_tag="VI520_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36412.1"
                     /translation="KVQSAWIGGDEKEFEADVMRKVVPAMMELIAAIGGVNLNLNKAT
                     SVIDQADAKVRSLADELGDIFDKI"
BASE COUNT          923 a         1585 c         1498 g         1024 t
ORIGIN      
        1 tctcgttgaa ttcggcgaag ttgtcgatca caaccacgat ctccgggaac aactccttgg
       61 gattcttgct gttgtaatcg gccagcgagt tgtacttggt gagtacctcc tggcgctcgt
      121 tcaggaagtt gcgcagcatc cgcatcagtt gatccacccg ggtgacctcg gccgcgtcga
      181 tggaggcgcc taagtgcggc aggtcctgaa tggacttcaa gccggcgcgg ccaaaatcca
      241 gggcgtacac gtggaggtcg ttcggggtgc gggtcgccgc cagaccgagc agcagggtct
      301 tcacgaaagt cgtcttgccc cggccggccg ccccaaagat cccaatggga ccatcggcga
      361 ggtccaggct catcaagcgc tgctccgccc ggtatggatt gtcgatcaac ccgagggtgg
      421 tctgcagcgg cctgggattg cgccaatcga aggttggcca gatctgcttc tcctgcgaat
      481 tgcggatcca cccgtccagc tgctcgctga tcacgatggt gcggtcggct gagagcgccc
      541 gttcggtatt gaggtaacgg gcgtccaccg gatcggtcag ggacagactg ggcggcagcg
      601 gctcaggcca gggtttggtc tgggtcggaa ccccatctcg ttcggcccgg atcacggtaa
      661 ttccgacaat ccaatccaac atggtggagg gatactcacc ggcttccagg ccgagcgcct
      721 cggccacttc cagtgcggag tacagcggtt ggtcctcgcc cgcctgttct cccggcccgg
      781 actgctcgtc gagccaaatc acatcgcgca gggtcacggt tcgatcgtcg ctgtagtctc
      841 cagctacccg cgccacgtgg accggcacga ggatatcgtt tcccacctgg atgtagcccc
      901 gacccccaat gttggggagg aaggccgcat ccggccggcc gagcagctct ttgctatctt
      961 caggcgtctc gacccgcaaa cagatccgga atttcatgtt ggcccgcatc tggtccgtga
     1021 ctaccccggc cggccgttgg gtagagagaa tcagggtgac cccaaacgcc cggcccagcc
     1081 gggtgatgct ttcgaactga tttcggtact ccggattcgc ggcgatcatc tcggcgaact
     1141 catccacgat gatgaacagg tgaggaaaag tccggggcag gctgtcgtcc gctccgagtc
     1201 ggggaatgac tttctttcgg tactcgacca gatccttggc cccgctcttg gccagaattc
     1261 ggcttcgttc atccatgacc gcttgaatgg cgacgaacat ccgctccacc gcatgggctt
     1321 ccaggttagt gaggatgtcg acgcagtgcg ggagtcggcg gaacggttcg aaagcggcac
     1381 cgcccttgaa gtccaccagg acgaaattca caatccgcgg atcgtatttg gcggccaggc
     1441 tggcgatcag ggtcagcagc agttcggatt ttcccgagcc ggtggttccc gcgatcatcc
     1501 cgtgaacccc gtcgccaccc tctttggccg aaatcaccag gctgcgtacg tcgcgcatgc
     1561 tggtcattcc gatgggcgca ctgagccact ctgagttttc gggccggata ctgcggcgcc
     1621 aattgtcctg aatcggcagc ttatcgaccg tgtccaactt gcggccctcg acccgcgact
     1681 ccatctgcag caggtccacc agtcgaggca gatccgcgcc aaacggccgt cgaatgtcca
     1741 gccggcgaat cttggccgcc agcagctgcc gggcatcgct ggcactggcc agatcggcgt
     1801 cgccaaggta acgagcagag ttcaccccca cctcggcata gcggaagact actttcttgc
     1861 cggccgccgc gacttccacc atcgcctggc agtcgctggg aatcttgagc gggtcattgg
     1921 ctaagaagat caccgaggct cctaaggcag gcccggcttg gttgaccgtg gcaatcagag
     1981 cctccgaggc gacttcggcc aggggcgaat cggccgggag atcccccgtg aggtcgacga
     2041 ccaccaacag atgcggcagg gaaatgtcgc cctcgcgccg ttcctcctca tccgtctcct
     2101 gcatccgcag ctggcgctgg tccagctcgc gcttcaagcg atcccagaag gcagtgacct
     2161 gctccttctc ggccgcaaaa cagagctgat cgagtcgagc cgggggaccg gactcgacgc
     2221 tctcatccag gccgacatct cggatgttgc agtgcggcag ccattcggcc cactgccaac
     2281 tcggctgagc ttgcgggtgc ccaatgacat acaacctggc atcggtcgcc gattggaagg
     2341 cggcgtaatg ggcgaccagc gcccggacaa agtccgcggt gttgcctggg ttcttcccga
     2401 agacgccgat cgagtggcgg gcttcgaccg aaccccccag attcccattg cgggcttcgc
     2461 tgcccccaac cgaatcggca acaaaccggc gcaggggaat tgtgatcggc acatcggtga
     2521 ggatctcgga atcttgagcc agcttgcgag cgtccttcgc cagcggccct tcatccagcg
     2581 gattgccggc ctgactcagg cggtagacca cgctggacgg ccgggtaccc attccgagac
     2641 gcacgacccc gaagtcgcgg tcgtcggtcc ttcgctccca cagccgcggg ccaaagcggc
     2701 tggtctcttt gcgggccgcg atttcataga ctgttgggat ctccgggtag ttgtggctat
     2761 agaagatgcg ttgtgcgttg tgggcccggt tcatgtcctg gcgcagctcg gccagttttt
     2821 cgagataggc cttcttcttg gcttcgagct ctttgcgctg ccgtctggcc ccgctgatcg
     2881 tcacgccgat cgtcagaacc atggccaggc cgatcgggat gatgaagaga acattcccgg
     2941 ttcccgagac caacacaaag ccaataatcg ttaccagcgg gatcagcagg ttcagtagat
     3001 cctgccccgc ccggcttccc tcttccggcg gctgagggat ttccacctcc ttgattggca
     3061 gctcgggctg aattcgaggg gggcgatcaa tgtactctgt gggcatcttc aagctctccg
     3121 gcgatgcagg gttaagcgag cgaaggcggg ctgtacattt gacagtggcg tctccgttcg
     3181 tctacaatgc gaactctcga atgaactgct tcgaatcgac ttcgaggcag ccatcaacaa
     3241 ttatctctta acttcgtccg cctgcgatcg ggcgggacgg gagtgggcaa cctcgaaaga
     3301 tgaaccgctt ttgcgagcga tgtgaacggg taactgtcga cggcaacctc tggtgtcaag
     3361 acccagactg cccggctgag gaaggttacg agctgctcgg ctacggagac ttcctgggcg
     3421 atctcaaagt taccaaactt gtgcgggtct ggcgcagtgc ggccgtctat gaagccgagc
     3481 ggggcaagct cccggtattg ctgaaagtgg cccacccgac cgatgcgtgc gtcgagcggc
     3541 tgaaacgcga agcaaccgcg cttcactcgc tttcgcccgc ccagtcgggc ccgctcgcct
     3601 tcgtgcgctc cttccttccc agctcccggc ccgccctccc gcggattctc ccgccctacc
     3661 caagcaaatt gaagcgaccg tttggcgaga tcacattccg gggtgagcct cgggtgtaca
     3721 gcgtctacca gcatgcccgg ggcaaaatcc tgagcgacgt cctgcttgag aacccgcaag
     3781 tttggcactc ccaggccgcc tgggtgatca gtgcggtggc ggaggccctg cgcccgttgg
     3841 tggccggcaa ccgttgccac ctgtgcctga cccctgacat catcttgatc gacaccgacc
     3901 ggcatggtaa tttgcgcccg atgctgctgg atctcggcct gatcctggag cctggaccaa
     3961 acggagcgaa ccaagatcgg aacaagctgt ctgagcccgc ctataccgct cccgagctgc
     4021 tggccaactc ccacgacgag atccccaccc tctcggcaga tgtctattcg ctcgggctca
     4081 tctacttcga gatgctggcc ggccgcccgg gattcgagag caaactgcgg cgggacgatc
     4141 agctgcgaga agacgtcact gagaatcgca aacccctgtc aatcgacagg cccgaattgg
     4201 atcaatctgg ggttaccgcg attgtggaac gggcggtggg ctcgaccggc cggtataaca
     4261 acgtgatcga actggccgcg gccctgacga agatctactc aaatcccccg cccgagcgga
     4321 ggccgatccc acgccggttg tacttcctga tagctttcct cggaacgttc ctattgattg
     4381 ccgccgtgtt tgccgccgct accctgctcc aggtcgtcgc tcgctaagat cggaccccga
     4441 gttcgccaga gagccatccg actgcgaacg gttcgccaga gctagcgccg cactccctct
     4501 ccgaagtatc gcttgcctgc gatgtcacct ttcttgtggt cgccgactgc ggaggccaag
     4561 tcgccgctga actcggtgca cagtttggcc aggggtttga tcttgttctt aatctgctcg
     4621 agataattgg ctagcgccaa ggtggtgccc atgctggcga acgccatggc gcgcaggatg
     4681 ttgattagga cttctagaac cttagccacc gtagttagga cgtcagccgc ggtcttaaat
     4741 cctttcgaaa catccgaaat gactttgtag tccatctgca cagttgtggt agccactgga
     4801 ttagtcctcc tgggggttcg ggctagatct tgtcgaagat atcgcccagc tcgtcggcca
     4861 ggctccgtac tttcgcatca gcctgatcga tgacactggt ggccttgttg aggttcagat
     4921 tgacgcctcc gatggccgcg atgagttcca tcatggctgg aacgaccttg cgcatcacat
     4981 cggcttcgaa ttctttctcg tcgccgccaa tccaggccga ctggaccttg
//
LOCUS       DATDPQ010000028         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_11064, whole genome shotgun sequence.
ACCESSION   DATDPQ010000028 DATDPQ010000000
VERSION     DATDPQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11064"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VI520_00615"
     CDS             <1..354
                     /locus_tag="VI520_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HLF36413.1"
                     /translation="GLAACGGPSTAAIAMSMSEFSFSPASASVPAGAEVTLTLTNSGT
                     IEHNWVLLDAGYQAAAPFDADDQANVVAETNVAASEVQTFTFTAPADAGSLQIVCSIP
                     GHLEAGMAASLTVDG"
     gene            complement(280..921)
                     /locus_tag="VI520_00620"
     CDS             complement(280..921)
                     /locus_tag="VI520_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36414.1"
                     /translation="MLTKLAIHEFMAAVEDRLGSPARLYLVGESSLVVEGWREWTDQL
                     VYTAEAAEHAQLTRIIEAVAARQGIRLVCESPADIIPVPPGHEGRARGWEIPGRSSPA
                     RLILYHFDPYSTAIRLIARGDEPDYRVALEFLRQGWIQLSELDRQLTELLPQFSAEAI
                     HQDPAEFRRKYKGLRQMWQALGNGSGSLLSAVDRQAGSHSRLQMAGDAAYDLE"
     gene            complement(927..1529)
                     /locus_tag="VI520_00625"
     CDS             complement(927..1529)
                     /locus_tag="VI520_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLF36415.1"
                     /translation="MEKIRIPAGPFLMGKDREVVELPDFSISKFPTTIEEYARFVEAT
                     GHRPPKWPGGKFPAESARHPVVNVSWEDALTYLNWVGARLPSEAEWEKAASGSQGWLY
                     PWGNEFIPDNCNSSESKSTGTRPVDAHPGGVSPYGVMDMAGNVWEWTSSLYEVGADWR
                     VLKGGSWDYKGFKDARAAARVYFGPTFRSGAIGIRPAWES"
     gene            complement(1556..2263)
                     /locus_tag="VI520_00630"
     CDS             complement(1556..2263)
                     /locus_tag="VI520_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36416.1"
                     /translation="MSEVDLLGQVVVADGLARVRTNGQVREYRVKELPESFIRWQLDY
                     KQRVYAAIELDQYVAFNAGHLPVVGTWDLGSAFPNLANKGVGFTPKDEFMDHYVKLVE
                     GAVAEIEQLPPHAVDETRRLRIATAREFYAHPEHIDWRRLGLLEIFEGSTYRNLSQNP
                     MAAVLWTGDSPIFVSFQVNCVVEVITPEHPRYRFSWAMRRLFEYEPFHVVQTMFPYSY
                     TFWVYDWKEKTPKRRYK"
     gene            complement(2315..>2839)
                     /locus_tag="VI520_00635"
     CDS             complement(2315..>2839)
                     /locus_tag="VI520_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HLF36417.1"
                     /translation="MAGTEYCRITPDGEVTPCPYMALPVGNVRGSSLTQIWQSAPQLL
                     ALRDPGQLKGRCGRCEYNELCGGCRCRAYARYGDILEEDPGCSYEPTGAKIVMSEIHW
                     SGEARVRLERIPIAFIRQKVKQGVEAYARRQSSRWITEEIMRQALAGFERPPAFQLDD
                     PTTLGAPAAVERLA"
BASE COUNT          530 a          930 c          837 g          542 t
ORIGIN      
        1 ggactggctg cctgcggagg tccgagcacc gcagcgattg ccatgtccat gtcggaattc
       61 tccttctcac cggcaagcgc ctcagtgccg gccggagccg aagtcacgct caccttgacc
      121 aacagtggga cgatcgagca caattgggtc ctgctggatg ccggctacca ggccgcggcc
      181 ccgttcgacg ccgatgacca ggcgaatgtg gtggccgaaa ccaacgtcgc cgcgagcgaa
      241 gtccagacct tcaccttcac tgctccggcg gacgccggct cactccagat cgtatgcagc
      301 atccccggcc atctggaggc gggaatggct gccagcctga cggtcgacgg ctgacagcaa
      361 ggagcccgag ccgttgccca gcgcctgcca catctggcgt agacctttat acttgcggcg
      421 gaactcggcc ggatcctggt gaatggcctc cgctgaaaac tgggggagca gctcggtcaa
      481 ttggcgatcc aactcgctga gctgaatcca gccctggcgg agaaactcca atgcgaccct
      541 gtaatccggc tcatcgcctc tggcgatgag ccggatcgcg gtactgtaag ggtcgaaatg
      601 gtaaagtatg agccgggccg gtgaactcct ccccgggatc tcccatcctc gggcgcgtcc
      661 ttcgtgccca ggtggaactg gaatgatatc ggccggcgat tcgcaaacca ggcgaatccc
      721 ttgtcgggca gccaccgcct cgatgattcg ggttagctgg gcatgctcgg ccgcctccgc
      781 ggtgtagacc agctgatccg tccattcgcg ccagccttca accaccagtg agctctcgcc
      841 gaccaggtag agccgagcgg gcgagcccag tcggtcctcc acggcagcca tgaattcgtg
      901 gatcgcgagc ttggtgagca taccgctcaa ctttcccagg cgggccggat accgatcgcc
      961 ccgctacgaa aggtcggacc aaaatagacc cgggccgccg cccgggcgtc cttgaatccc
     1021 ttgtaatccc aggaaccccc cttcaacacc cgccagtctg cgccgacctc gtagagactg
     1081 ctggtccact cccacacgtt gccggccatg tccatcacgc cgtatggaga aaccccgccc
     1141 gggtgagcgt ccaccggccg ggtgccggtg cttttcgatt cgctggagtt gcagttgtcg
     1201 gggatgaatt catttcccca cgggtacaac cagccctgcg agccgctggc tgccttctcc
     1261 cattcggcct cgctcggcaa tctagcccca acccaattca aataggtcag ggcatcttcc
     1321 caactgacgt tgacgaccgg atgcctggcg ctctcggcag gaaacttacc tcccggccac
     1381 tttggcggcc gatggccggt tgcctccacg aagcgtgcat attcctcgat agtcgtcggg
     1441 aacttggata tcgagaagtc tgggagctcg acgacttccc tgtcctttcc catcaggaag
     1501 ggcccggccg gaatccgaat cttctccatc gggccggcag cctgaaggag cggcgctact
     1561 tgtagcgccg tttgggcgtc ttttccttcc aatcgtagac ccagaaggtg taggagtagg
     1621 ggaacatagt ttggaccaca tgaaagggct cgtattcaaa caggcgtctc atcgcccagg
     1681 agaagcggta gcggggatgc tcgggagtga ttacctctac gacgcaattg acctgaaagc
     1741 tgacgaagat cggcgagtcg ccggtccaca aaacagctgc catcggattc tggctcaagt
     1801 tccggtaggt gctcccttcg aagatctcga gcagcccgag ccgcctccag tcgatatgct
     1861 ccgggtgggc gtagaactcg cgggcggtcg caatcctcag ccgccgggtt tcgtccaccg
     1921 catgcggggg gagttgctcg atctcggcga ccgcgccttc gacaagcttt acgtaatgat
     1981 ccatgaactc gtccttgggt gtgaacccaa ctcccttgtt ggcgaggttg gggaaggccg
     2041 agcccaaatc ccaggtaccg accaccggga gatgacccgc attgaaggcg acgtattggt
     2101 cgagctcgat cgccgcgtaa acccgctgtt tgtagtccag ctgccagcgg atgaaacttt
     2161 cggggagctc ctttacccga tattcccgta cctgcccatt cgtgcgaact cgggcaagtc
     2221 cgtctgccac aacgacttgg ccgagcagat caacttccga catcagtcct ctccttcatg
     2281 gcaatctggc tatgcgcagt ctgcctagtg gcgatcaggc cagccgctcc accgccgccg
     2341 gagcccccag ggtggtcggg tcatcgagct ggaaggctgg aggccgctca aatccggcga
     2401 gcgcctggcg catgatttcc tccgtgatcc agcggctaga ctgacgtcgg gcgtaggcct
     2461 ccaccccctg tttcaccttc tggcgaatga aggcgatcgg gatccgctcg aggcgaaccc
     2521 gcgcctcccc gctccagtgg atttccgaca tgacgatctt cgccccggtc ggctcataac
     2581 tgcaccccgg gtcttcttcc aggatgtcgc cgtagcgagc ataggctcgg cagcggcaac
     2641 ctccgcagag ctcgttgtat tcacagcggc cgcagcggcc cttgagttga ccgggatccc
     2701 gcagcgccaa cagctgcggc gcgctctgcc agatctgggt caacgagctg ccgcggacat
     2761 ttcccaccgg cagagccatg tagggacagg gagtcacctc gccatccgga gtgatccggc
     2821 agtattcggt ccccgccat
//
LOCUS       DATDPQ010000029         4700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1841634, whole genome shotgun sequence.
ACCESSION   DATDPQ010000029 DATDPQ010000000
VERSION     DATDPQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841634"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VI520_00640"
     CDS             complement(<1..199)
                     /locus_tag="VI520_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HLF36418.1"
                     /translation="MDSSSAPETKVETSAQEAGRYFRHMAQFVGFTKEDAAAIKQSQL
                     VIEKFIPEIVGKFYDHLLHYPP"
     gene            complement(217..1482)
                     /locus_tag="VI520_00645"
     CDS             complement(217..1482)
                     /locus_tag="VI520_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36419.1"
                     /translation="MIRLSAERTAPSSSFRTAAWLAWSLWALAMALAGLGLVFLALSY
                     STPVPPRWGPRGFPVIAALVSSTIGALIVSNRPRHPVGWIFAWLGLLSGAQVFVEEYR
                     VYALLTMAGSLPGGEISAWIANWIWVPAIAPAITFLFLIFPTGQLLSPAWRPVAWFAA
                     IGAAGTTFLAAFVPGPFEQFGYLNNPFGIEALGNLGISASSALVPLMIAVLASVASLI
                     LRIRRSSGEERLQLKWFVYAAVLVALALPGGALYQSFPAGPIFMISGMAATQVAVGVA
                     ILKYRLYDIDLLINRTLVYGSLTLVLAAVYFAGVALLQASFRALTGQDSPLAVVLSTL
                     AIAALFNPLRGRFQSAIDRRFYRSNYDASRILSAFSAHARDEVEVSRLSASLIEAADQ
                     AMQPTTITLWLRDSGEPSRQEPISHSLLP"
     gene            complement(1479..2939)
                     /locus_tag="VI520_00650"
     CDS             complement(1479..2939)
                     /locus_tag="VI520_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36420.1"
                     /translation="MSEGQRTKTNTISDGAHPLLVLGAVVVIVLPAFVFLGARLLLPG
                     DGAQLQFEPFREVERGLLVNVSRPRPGGLQDGDRVIALAGRSVDQWIAMAFRGTKNVP
                     ARHGPVVYTVLRNNEVDELSVALSRQPLPAIIGRNWTSYLFLTYLWAIGLVVFALRPR
                     QTSAQGVLLFSSLLMGSGLIFFLLIQPSDLMHGWLLPLWTWGALILYIVFAGAAIHFV
                     LIFPSKRAVLRHRPSLPLAGYLGSLLLWVLSGALRWPEATTPSARLYLMVTATNLLSA
                     TAMPLSVLIVLHGYRTEYDQVERRQVRWFLWAIVVAVIPWQALSVIPELLGYRALIPQ
                     SLTSLLWLAIPTALAIAILREGLFDVDVIINRTLVYGGLTGMLAALYFAGVAFLQSAF
                     RGAAGQESPLAIVLSTLGIAALFNPLRRRIQGFIDRRFYRRKFDAALLLAEFGRTVRD
                     EVDLGRMQETLLGTVQDTLQPAHVSLWLRSGPGDLK"
     gene            complement(2967..4256)
                     /locus_tag="VI520_00655"
     CDS             complement(2967..4256)
                     /locus_tag="VI520_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36421.1"
                     /translation="MTTARFATTMPASLDKRLLAARTAWVAVTVLVVALVALGVPGLY
                     ARLIAGVDERSVTDLGWTLPGYGTYLTGLYLLVVVAHLVIGAIVFWRRSNEWIALALA
                     FALVCNGALLPLSLIYPSGSVSPVVQLLVGLVTYIGLVTGLSLLYLFPDGRFIPRWTQ
                     LMVIAWAGLLFFAIFTPTSALSLAAWPIPLQLLVLVVWSGVGLFAQLHRYLEVSSPLQ
                     RQQTKWAILGLGLAVLGPFGYFLPFVILPALSDPEVPNILYQRIGPSLFTFSLVFRLI
                     GLTGFTLALLIFPASFVVAILRYRLWDIDVIINRTLVYGALTSTLALVYLGGVALLQT
                     MFYGLTGQGDQLAIVATTLAVAALFNPLRRRIQAVIDRRFYRRRYDAARALAAFSGTV
                     QEEVDLEDVSQALLRVVEETVQPLQTSIWLRDIRPGS"
     gene            complement(4275..>4700)
                     /locus_tag="VI520_00660"
     CDS             complement(4275..>4700)
                     /locus_tag="VI520_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF36422.1"
                     /translation="FLKSLPPVDNELPERDLGLFYQLAILSDPSLIPAEVIDHTAPRP
                     PAPEPAVSAEYGKYLATACTICHGPDLSGGTAAGAGLDLTGGGNLTEWTEAEFMHALR
                     TGERPRGEDIDPSRMPWKRVGKLTDDELRAIWLYLQTLQ"
BASE COUNT         1066 a         1390 c         1498 g          746 t
ORIGIN      
        1 ttgggggata gtgcagcaag tgatcataga actttccgac gatctctgga atgaacttct
       61 cgataacaag ttgactctgc ttgatagcgg ccgcgtcttc ctttgtgaag ccgacgaatt
      121 gggccatgtg gcggaagtag cggccggcct cttgggccga ggtctcaact ttggtttcgg
      181 gtgcagatga actatccatg gtttcctctt tcggtctcat ggaagcaatg agtgcgatat
      241 tggctcttgc ctcgatggct ccccggagtc ccgaagccag agagtgatag tagtcggttg
      301 cattgcctga tcggccgcct ctatcagcga agccgatagc ctgctgacct cgacctcatc
      361 tcgggcgtgt gcgctgaaag cggagagtat ccgcgaggca tcataattgc tccggtagaa
      421 acggcggtcg atagccgatt gaaagcggcc gcgcagcggg ttaaagaggg ctgcgatggc
      481 tagcgtcgag agcaccacgg ccagagggga atcctgcccc gtgagggccc ggaagctagc
      541 ctggagaagc gctacgccag cgaagtacac cgcggccaac actagggtca ggctcccgta
      601 gaccagtgtg cggttgatga gcagatcgat gtcgtagagc cggtacttga ggatcgctac
      661 ccccaccgcc acctgggtcg ctgccattcc cgaaatcatg aatattggcc cggctggaaa
      721 gctctgatat aaggcgccgc cgggcagggc cagcgccaca aggactgccg cataaacgaa
      781 ccatttgagc tgcaggcgct cctctcctga cgaacggcgt atccgtagga ttaaggaagc
      841 cacggacgcc aagactgcga tcataagggg caccaaggcg gaggaagccg aaatcccaag
      901 gtttcctaaa gcctcaattc caaagggatt gttaaggtag ccgaactgct cgaacggacc
      961 tggtacgaag gcggcgagaa aagtcgtccc tgccgcaccg atcgctgcaa accaagccac
     1021 gggtcgccag gcgggagaaa gaagttggcc ggtggggaat atcaggaaga gaaaagtaat
     1081 ggccggggcg atggccggga cccagatcca gttcgcgatc caggcggata tctctccacc
     1141 tggcagcgag ccggccattg tcagcagggc ataaacccgg tactcctcca cgaacacctg
     1201 ggctccggaa agtaggccaa gccaggcgaa aatccagcca accggatggc gtggacggtt
     1261 tgaaacaatc aaagcaccga tggtcgagga aaccagggcg gcgatcaccg ggaacccgcg
     1321 cgggccccag cggggaggta cgggagtgga gtaactgagc gccagaaaca cgagcccgag
     1381 ccctgcgagg gccatggcga gcgcccacag tgaccaagcc agccaagcag cggttctgaa
     1441 ggagctactc ggtgcggtcc gctcggcact tagccggatc atttcagatc ccccggaccg
     1501 gatcggagcc acaaagacac atgcgctggc tgcagggtgt cctgcaccgt gccgagcaaa
     1561 gtctcctgca tccttcccag atccacctcg tcccggaccg tccgtccaaa ctcggccagc
     1621 agcagggccg cgtcgaactt gcgccgatag aaacggcgat cgatgaagcc ctggattcgg
     1681 cggcgcaacg ggttgaagag tgccgcgatc ccgagtgtag aaagtacgat cgccagcggc
     1741 gactcctgtc cggcagcccc tcggaaggcg ctttgcaaga acgcaacgcc cgcaaagtac
     1801 agcgcggcca acatcccggt cagaccgccg tagactagcg tccggttgat gatcacgtcg
     1861 acatcgaaga gaccctcccg caggatagcg attgccaagg ccgtggggat cgcgagccac
     1921 agcagactgg tgagggactg ggggatcaat gctcggtagc ccagcagctc cggaatcacc
     1981 gaaagggcct gccaggggat cactgctaca acaattgccc agagaaacca gcgcacctgt
     2041 cggcgttcga cctggtcgta ctcggttcga tacccatgga gcacgatcaa cacgctcaga
     2101 ggcatggcgg tcgccgacag caggttcgtc gcggtgacca ttaggtagag gcgcgcggaa
     2161 ggggttgtgg cctcgggcca ccgaagggca ccggaaagta cccaaagcag cagagacccg
     2221 aggtagccgg ccaagggcag ggagggccgg tgccgaagga cggctcgctt gcttgggaag
     2281 atgagcacga agtgaatggc cgcgcccgca aatacgatgt agaggatcaa cgctccccaa
     2341 gtccacagcg gcaacaacca gccgtgcatc aagtcgctag gttgaatcaa caagaagaag
     2401 atcagtccgc tgcccatgag caggctgctg aacagcaaga ccccttgtgc actggtttgc
     2461 cttggccgca gtgcaaatac cactaggccg atcgcccaca ggtaggttag gaagagatag
     2521 ctggtccaat tacgtccgat gatcgccggc agaggttgcc ggctgagcgc cacgctgagc
     2581 tcatcgacct cgttgtttct taggacggtg tacacgaccg gaccatgacg ggctggcacg
     2641 ttcttggttc cacgaaatgc catggctatc cactgatcga ccgagcgacc cgctagggcg
     2701 atgacccggt cgccgtcctg caagcctcca ggtcgcgggc gactcacatt tacaagcaat
     2761 ccgcgctcca cctcgcggaa cggctcgaac tgcagctgag ccccatcgcc cgggagcagc
     2821 aggcgggccc cgaggaacac gaacgccggc agcacgatca caaccactgc gccgaggacg
     2881 agcagtggat gcgcgccgtc agagatggta ttggtcttcg tgcgctggcc ctcgctcacc
     2941 gacgacacag atgggcccct tctctttcag ctacctggac ggatgtctct caaccaaatc
     3001 gaagtctgca gcggctggac cgtctcctcc acgaccctca gcagagcctg gctcacatct
     3061 tccagatcca cttcttcctg cacggtgcca ctgaaggcgg ccagggcccg ggccgcgtcg
     3121 tagcgccggc ggtagaagcg gcggtcgatg accgcctgga ttcggcgccg cagggggttg
     3181 aagagtgccg cgacggcgag agtggttgca acgatggcaa gctggtcacc ctgtccggtt
     3241 aggccgtaga acatggtctg gagcagcgcc acgccgccca gatacaccag cgccaacgtg
     3301 ctcgtcagtg ccccgtatac gagagtgcga ttgatgatga cgtcgatatc ccaaagccga
     3361 tagcgcagga tcgcgacgac gaacgaagcc gggaagatca agagggccag ggtgaagccg
     3421 gtaagaccga tcaggcggaa gaccaaggag aaggtgaaga gcgagggccc tatccgctgg
     3481 tacaggatat tggggacttc cggatcgctc agtgcgggaa ggatcacgaa gggaaggaag
     3541 taaccgaagg ggcccagaac agccagcccg agtccaagga tggcccattt ggtctgctgg
     3601 cgttgcagtg ggctggagac ctcgagatag cggtgcagtt gagcgaagag accaacgccc
     3661 gaccaaacca cgagcaccag cagctgcagc ggaattggcc atgccgcgag gcttagcgcg
     3721 gaagtgggcg taaagatggc gaagaacaac aacccggccc aagcgatgac catgagctga
     3781 gtccagcgag ggatgaaacg cccgtccgga aagaggtaca gcaagctcag tccagtgacc
     3841 aggccgatgt aggtcaccag acccaccagc agctgcacga cgggcgagac gctgccggaa
     3901 gggtaaatca gagagagcgg caacaacgct ccgttgcaaa ccagtgcgaa agcaagggcc
     3961 agggcgatcc attcgtttga ccgacgccaa aacacgatgg ccccaatgac caggtgggca
     4021 acgacgacga gcagatagag gccggtgagg taagtgccgt agccgggcag ggtccagccg
     4081 aggtcggtca ccgatcgttc gtcgacaccc gcgatcagcc gtgcgtacag gccgggcacg
     4141 cccagggcga ccagggctac gaccaggacg gtcaccgcca cccaggccgt gcgggccgcg
     4201 agcaggcgct tgtccagcga ggccggcatg gtggtggcga accttgctgt cgtcacggtc
     4261 gtttgagcct cctcctactg aagcgtctgc aggtaaagcc agatcgcccg cagctcatcg
     4321 tcggtcagct tgcccacccg tttccagggc attcgactgg gatcaatatc ctcaccgcga
     4381 ggccgttcgc cggtcctaag cgcgtgcatg aattcggcct cggtccactc agtcaagttg
     4441 ccgcctccgg tcaaatccaa gccagcaccg gctgcagtgc caccgctcag gtccggtccg
     4501 tggcagatgg tgcaggcagt cgccaggtat ttgccgtact cggcactgac ggcaggctca
     4561 ggtgccggtg gacggggagc tgtgtggtcg attacttcgg cagggatcag agaggggtca
     4621 ctcaggatgg caagctggta gaacagtccc agatcccgct ccggcagctc attgtccacc
     4681 ggaggcaggc tcttgaggaa
//
LOCUS       DATDPQ010000030         5460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_795490, whole genome shotgun sequence.
ACCESSION   DATDPQ010000030 DATDPQ010000000
VERSION     DATDPQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795490"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VI520_00665"
     CDS             <1..682
                     /locus_tag="VI520_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF36423.1"
                     /translation="VEKGFILGEGDGGGGWAGHYLLLTGYDEASQVFIIQDTNPGTGG
                     PDREISYAELDDGWQAFNRVYLVVFPAGEDARIQALLGTDADLAQNRAEAIDTAQAEI
                     EADPQDPYAWFNLGSNLITFERYGEAATAFDNALSIGLPWRFTRYQFGPYIAYFNQGR
                     FQDVIDLAEATLYRTYKAEESLLWRGWARFRLGDLNGAVADFREALDYNPNYLDAQYA
                     LEYVGASR"
     gene            794..1294
                     /locus_tag="VI520_00670"
     CDS             794..1294
                     /locus_tag="VI520_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HLF36424.1"
                     /translation="MLISEKLNQAINGQIGSELGASNQYLQVAAHFGREDLPELAGFF
                     FRQSDEERGHALKFVQYILDAGGTVAIPAVSAPTQEIKSAEQAAQLSLDSEKRVTDQI
                     NALLQIATAEKDFAALRFLGWFAEEQIEEVSTMNELLNTIRRAGDQILMVEAALARRG
                     VPHGDS"
     gene            1519..2109
                     /locus_tag="VI520_00675"
     CDS             1519..2109
                     /locus_tag="VI520_00675"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HLF36425.1"
                     /translation="MSHRMRNVILVGFGLAVALPAGWYLLSPLFIDRVVEEEFPIVPA
                     AATAAMEEAAEATAAMEDAMAEPEKTMEDPLPADEMADMHILAQGEFYNLAHEGQGTA
                     TIYELADGSRVLRFEDFEVLNGPELHVWLVPVDPVPDTVGVEIAGYFDLGPLKGNIGD
                     QNYDLPADLELSDFKSVVIWCQPFRVPFNAAPLVAP"
     gene            2143..2832
                     /locus_tag="VI520_00680"
     CDS             2143..2832
                     /locus_tag="VI520_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36426.1"
                     /translation="MSGPKRILVVEDEDTIREVIRRYLEREGFRVDEAADGYAALDRI
                     AEAEPDLIVMDLMLPGIDGLTLTRQLRQRSRVPIIMLTARGDTSDRIRGLDLGADDYL
                     AKPFSPRELVSRIQAVLRRGAPAEPARLRFGMVEMDVGSRTVTRGSQNVKLTAREFDL
                     LWFIASHPGQVFTRERLLDNVWGVDFAGDPSTVTVHIRHLREKIETDPAEPQHLITVW
                     GVGYRLESGDG"
     gene            2825..4081
                     /locus_tag="VI520_00685"
     CDS             2825..4081
                     /locus_tag="VI520_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF36427.1"
                     /translation="MASGRLGFVGSILLALSITLGLTVLIMRPPAQDVLQLALVFGAT
                     GLGASLLGFISHRLGWWRRASSLTQSLTIGHLLAALVGLIIVLVAAGLMFINKHDLTL
                     AGLLLLFASSVAVSFGYFLSNSITERLKLLEGGARRLSEGDFGVRVNLSGMDEVAQLG
                     RAFNRMAERLEQAEAESARLEAARRDFIAAASHDLRTPLTSLRAMLDAIADGVVEQPD
                     EYIQRSLRVIAQLTSLTDDLVELARLDSGGAPLQRSLGSIGKIVTAAVEGAALRAKVK
                     GVRLTGSVSPNEAPLPIAEEAITRVMQNLLDNAIRHTGEGGEVQVEVRESNGMVEVSV
                     QDTGEGILPEHLPHVFERFYRGEKSRSREGMETGGVGLGLAIAKELVEAHGGRIWAES
                     SPSRGTRISFMVPREGAAAHRVQTST"
     gene            4156..4386
                     /locus_tag="VI520_00690"
     CDS             4156..4386
                     /locus_tag="VI520_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36428.1"
                     /translation="MRAGGEATRGIVAWLRRAGLGSVAAEALEAFSPLATIGAQLGYL
                     AAPFIDGMGDLGRRLEDPAELIRQLRDEAPDP"
     gene            4383..5132
                     /locus_tag="VI520_00695"
     CDS             4383..5132
                     /locus_tag="VI520_00695"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HLF36429.1"
                     /translation="MTVGGLQGLVVLAVFVALMVGFLAVNQRWPAHFRRIPAYEALRQ
                     AIERAVEAGERVHVSLGTGSVIGPESAPALAGLAALTRIARATSISDRPVVVTTGDGA
                     MAILARDSLRSTYQEVGASELYEPTSGRLLGATPLSYAAGLPAVLANERVSVHVLSGA
                     FGVEAGLAADFGERAGLFVIGGSDDVQAQALFYAVAQHPLIGEEVFASGAYLEVGTMH
                     TASLRAQDFVRFGLIVLILLGVIVRTLEGPL"
     gene            5129..>5460
                     /locus_tag="VI520_00700"
     CDS             5129..>5460
                     /locus_tag="VI520_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36430.1"
                     /translation="MRVLRLSAIWAALALGVGLFVLLGYFVDLGFIQNFRLTFLRWAV
                     LLAAAALVVGLANLLAVHWRKVSLTEEGWIYSGVLILSFTVTLVLALVFRPDGQAALF
                     IFNTFQLPL"
BASE COUNT          964 a         1649 c         1795 g         1052 t
ORIGIN      
        1 tgttgagaaa ggcttcatcc tgggtgaggg cgatggcggc gggggctggg ccggccacta
       61 tctgctgctg accggctatg acgaggcctc ccaggttttc atcatccaag acaccaaccc
      121 tggcaccggc gggccggacc gtgagatctc atatgccgaa ctcgacgacg gctggcaggc
      181 cttcaaccgg gtttacctag tcgttttccc agccggtgag gatgccagga ttcaagcgtt
      241 actcgggacc gacgcagatc tggcccagaa tcgggccgag gccattgata ctgcgcaggc
      301 cgagatcgag gccgatcctc aggacccgta cgcctggttc aatctcggga gcaacctgat
      361 cacctttgag cgctacggag aggcggccac cgcctttgac aacgcattgt cgatcggact
      421 tccctggcgg tttacccgct atcagttcgg cccctacatc gcctacttca atcagggccg
      481 cttccaggac gtgatcgacc tggcggaggc taccctgtac cggacttaca aagctgagga
      541 gtcgctgctg tggcggggct gggcgcgttt ccggctgggc gatctcaacg gtgcggtcgc
      601 agattttcgc gaggcgctgg actacaaccc gaactacctc gacgcccaat acgccttgga
      661 gtatgtcggg gccagccggt gagctgcacc ggtgtcgggc gggagcaatt ccgctccggc
      721 gctggggtat gattgagtgg gccgagcgca ctcccgagcc cgagcaccct cagatcccaa
      781 taggtgactg cccatgctaa tcagtgagaa gctaaatcaa gccatcaacg gacagatcgg
      841 aagcgagcta ggggcttcca accaatacct gcaggtcgcg gctcattttg gaagagagga
      901 tctccccgaa ctggcggggt ttttctttcg ccaatcggat gaggagcggg ggcacgcact
      961 caagtttgtg caatacattc tggacgccgg aggcacagtg gccatcccgg ccgtctcggc
     1021 tccgacccaa gaaatcaagt ccgccgagca agccgcccag ttatcgctcg actcggagaa
     1081 gcgagtgacc gatcagatca atgccctgct gcagatcgcg accgcagaga aggatttcgc
     1141 cgcgctgcgg ttcttaggtt ggttcgccga ggagcagatc gaggaagtct cgacgatgaa
     1201 cgagttgctg aacacgatcc gccgggcagg cgaccagatt ctgatggtgg aagccgccct
     1261 ggcacgccgg ggagttcccc acggcgactc gtaagcgcgc gcatcggaaa cccgttactt
     1321 acccggctac ccaagtgacc ggtggacgaa accgctctcc aactcacgga gagcgtttct
     1381 ttttcgacct accccggcgg gccggccggt ctgggcggcg aactcctccg ccactcagca
     1441 cacggtaaga aagatttaag catttgtgac gagactggga gcgagcaatt gaccccattt
     1501 gggaaggaga ctcaacccat gagccatcga atgcgtaatg tgatcctggt cggcttcgga
     1561 ctggcggtgg cgctgccggc aggttggtac ttgctttcgc cgctcttcat tgatcgggtt
     1621 gtggaggagg agttccccat tgtcccggcc gcggcaaccg ccgcgatgga agaagcggcc
     1681 gaagcgacgg cggccatgga agatgccatg gccgagcccg agaagacaat ggaagatccg
     1741 ctgccggccg acgaaatggc cgatatgcac attctggcac aaggcgagtt ctacaacctg
     1801 gcccacgaag gccagggaac cgccacgatc tacgagctag ccgacggctc gcgggtgctg
     1861 cgcttcgagg attttgaggt gctcaacggt ccggaattgc acgtttggct ggtgccggtc
     1921 gacccggtgc cggatacggt cggtgtggaa atcgccggct acttcgattt gggaccgctc
     1981 aagggcaata tcggcgacca gaactacgat ctgcctgctg atctcgaact tagcgatttc
     2041 aagtccgtag tgatctggtg ccagccattt cgggttccgt tcaatgccgc accgctggtc
     2101 gcgccatgaa ttcgagcaga tctgatacga tccggcccag acatgagcgg gccgaagcga
     2161 atcctagtcg tcgaagatga ggacacgatc cgcgaagtca ttcgccgcta cctggaacgg
     2221 gagggctttc gggtcgacga ggcagccgac gggtatgccg cgctggatag gatcgcggag
     2281 gcggagccgg atctgatcgt catggacctc atgctcccgg ggatcgacgg gcttaccttg
     2341 actcgccagc tgcgtcagcg ctcccgcgtg ccgatcatca tgctgaccgc gcggggcgac
     2401 accagcgacc ggatccgtgg gctggatttg ggagcggacg actacctggc caagccgttc
     2461 agcccccgcg agctggtctc ccggatccaa gccgtcttac ggcggggcgc acccgccgaa
     2521 cccgctcgcc ttcgtttcgg catggtcgag atggacgtgg gctcgcgcac ggtcacccgc
     2581 ggctcgcaga acgtcaagtt gaccgctcgc gagttcgacc tgttgtggtt catcgccagc
     2641 catcccggac aggtcttcac ccgcgaacgg ctgctggata acgtctgggg agtggatttc
     2701 gccggcgatc catcgacggt cacggtccac attcgccacc tgcgggagaa gatcgaaacc
     2761 gatccggcgg aaccccaaca cctgatcacg gtgtgggggg tcggctatcg cctggagagc
     2821 ggggatggct agcggcaggt tgggctttgt cggcagcatc ctgctggcgc tctcgatcac
     2881 gcttggcctg accgtgctga tcatgcgccc gccggctcag gatgttctgc agctagctct
     2941 ggtttttgga gccactggcc tgggggcctc gctgctgggt ttcatctctc accgcctcgg
     3001 ctggtggcgg cgcgcctcca gcctgaccca gtcgttgact attgggcact tgctcgcggc
     3061 actcgtgggg ctaatcatcg tgctcgtggc cgccgggctg atgttcatca acaaacacga
     3121 tctcaccctg gcaggtctac tgctgctgtt tgccagcagc gtcgcggttt cctttggata
     3181 ctttctgtct aactcgatta ctgagcgatt gaagctgttg gaggggggcg cccggcggct
     3241 gagcgagggg gatttcgggg tacgggtcaa cctcagcggc atggacgaag tcgcccagtt
     3301 agggcgggct ttcaatcgga tggctgagcg actggagcag gccgaggcgg aatccgctcg
     3361 gctggaggcg gcccggcgag atttcatcgc cgcagcgtcg cacgatttgc gcactccgct
     3421 gacctcccta cgagcgatgc tggacgccat cgccgatggg gtggtcgagc agccggacga
     3481 gtacattcaa cgcagcctgc gggtgatcgc tcagctaacg agtctgaccg atgatctggt
     3541 cgagctcgcc cgcctggaca gtggtggcgc gccgttgcag cgctccctcg gttcgattgg
     3601 caaaatcgtg accgcggcgg tcgagggggc cgcattgcgc gccaaagtga aaggcgttcg
     3661 cctgaccgga agcgtctcgc ccaatgaggc tccgttgcca attgccgaag aggcgatcac
     3721 ccgcgtgatg cagaacttgc tggataacgc gatccgccac accggcgaag ggggagaagt
     3781 gcaggtcgag gtgcgcgaga gcaacgggat ggtggaagtg agtgtgcagg acaccgggga
     3841 ggggatcctc ccagagcatc tgccgcacgt attcgaacgt ttctatcgcg gtgaaaaaag
     3901 ccgttcccgg gaaggaatgg agacaggcgg cgttgggctt ggcctggcaa tcgccaagga
     3961 gttggtggag gcccacggcg gccggatctg ggcggagagt tcgccgtcgc gcggcactcg
     4021 gatcagcttc atggtgcccc gagaaggggc ggcggcgcat cgagtccaga cctcaaccta
     4081 gggccaaaaa gctaagcagg gcccgcgcag gcgccgcgcg tagcgcaaag aaaccgatgt
     4141 gctatactgc gcgcgatgag agccggggga gaagctacgc gggggatcgt ggcttggctg
     4201 cgccgcgccg gtttgggttc agttgcagcc gaggcgcttg aggcgttctc accgctcgcg
     4261 acgatcggcg cccagctcgg atacctggca gcaccattca tcgacggcat gggggacctc
     4321 ggtcgccggc ttgaagaccc cgccgagctg atccggcaat tgcgcgacga ggcgcccgac
     4381 ccatgactgt cggcggactg cagggactgg tcgtgctggc cgtctttgta gccttgatgg
     4441 tggggttcct ggcagtcaat cagcgctggc cggcccactt tcgacgcatc ccagcctacg
     4501 aggcactccg ccaagcgatc gaacgggcgg ttgaggccgg cgagcgggtc catgtttcgc
     4561 tcggtactgg atcggtcatc ggcccggaga gcgcgccggc cctggccggg ttggccgccc
     4621 tgacccggat cgcccgcgca acctcgatca gcgatcgtcc ggtagtggtg actaccggcg
     4681 acggtgcgat ggccattctg gcccgcgaca gcctgcgttc cacctatcaa gaggtcgggg
     4741 catccgagct gtacgagccg acctcgggcc ggctgctggg cgccactcca ctttcgtatg
     4801 cggctggact gccggcagtg ctggccaacg agcgagtctc ggtccacgtg ctcagcggcg
     4861 ctttcggagt cgaggccggc ctggcagccg atttcggcga acgggcgggg ctgttcgtca
     4921 tcggggggtc cgacgacgtc caagctcaag cgctcttcta cgcagtcgcc cagcacccac
     4981 tcatcggcga ggaagtcttc gccagcgggg catacctgga agttggcacg atgcataccg
     5041 ccagcttgcg cgcccaggat tttgttcgtt ttggactgat tgttctgatt ctgcttggag
     5101 tcatcgttcg gaccctcgag ggcccgctgt gagagtgctc cgcctctccg cgatctgggc
     5161 cgccctcgcg ttgggagtcg ggctgtttgt gttgctgggc tatttcgtcg atctggggtt
     5221 catccagaat tttcgcctga cctttcttcg ctgggccgtg ctcttggcgg cagccgcgtt
     5281 ggtggttggg ctggccaacc tgcttgccgt ccactggcgc aaggtcagcc ttactgagga
     5341 aggctggatc tacagcgggg tgctgatcct ctctttcacc gtgactttgg tgctggccct
     5401 ggtcttccgc cccgacggcc aggctgccct cttcatcttc aacacctttc aactgccgct
//
LOCUS       DATDPQ010000031         3081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3150055, whole genome shotgun sequence.
ACCESSION   DATDPQ010000031 DATDPQ010000000
VERSION     DATDPQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3150055"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VI520_00705"
     CDS             <1..186
                     /locus_tag="VI520_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF36431.1"
                     /translation="SALIEYAFVRLRLARIVATTTYDNTASVGVMRKLGMRVERNPLA
                     EPPWLQVVGVLEAVRRP"
     gene            206..724
                     /locus_tag="VI520_00710"
     CDS             206..724
                     /locus_tag="VI520_00710"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HLF36432.1"
                     /translation="MTDPTISTLSELKIVIDGSWSELEAFLAGLTEQQASVQDDQGWT
                     VTDHVTHIAVWEDSVAILFRGKPRHEALGISESYYAQASFDEINEQIRQRMGHMPLSL
                     AIEQLRQVHLTLMANFDSLSEAELSMAVRDFFSEAPRSDERRVIDFFQDNTADHFKEH
                     LGWMQTLLKRAS"
     gene            866..1375
                     /locus_tag="VI520_00715"
     CDS             866..1375
                     /locus_tag="VI520_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF36433.1"
                     /translation="MAHPLVDQLRFTRSEWLRGLEGISDEDGSRHFGPMNCISWIVGH
                     LAWQEQRYFLQRAQARLIFPELITAYAYGAPMSTPALSDTSRFWNEVIQTSAPYLDGL
                     TTVSLQEELLLEGKPVGQSVGSAMRRMTYHYWYHIGEIQAIRQMLGHPGLPEYVGDIE
                     TLAPYRPEQ"
     gene            1577..2083
                     /locus_tag="VI520_00720"
     CDS             1577..2083
                     /locus_tag="VI520_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36434.1"
                     /translation="MIRIWLIGISLAVTAACNPSQQSPMVDGSGATPSATATESPSAA
                     PVLSPTPGSRPAIVETTTTGLIINGNVRLENGTPLANVQICRNFAAYNGKTVAVTDER
                     GRYEAALAFIPGIETIGVWPKLAGYTFDPPFYDWVHYYGLETRTLDFVAKISYGTPGP
                     PFPVECDP"
     gene            complement(2606..2794)
                     /locus_tag="VI520_00725"
     CDS             complement(2606..2794)
                     /locus_tag="VI520_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36435.1"
                     /translation="MPSVGSGQRGSAVGEGAGGTAVDAAVGAAGEAEVSEGVIGGWPC
                     GAQADRTWKAIAANPTNA"
     gene            2799..>3081
                     /locus_tag="VI520_00730"
     CDS             2799..>3081
                     /locus_tag="VI520_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36436.1"
                     /translation="MLTHEEDGYCFLYPDGLLRVDSSQHAVCLVPEGDLLGCDSFVAA
                     VEVTDAEGRTAAQIADEKVLSEGSVGADRCTAPIAGIQTVVLPQVADQGL"
BASE COUNT          590 a          948 c          939 g          604 t
ORIGIN      
        1 agcgccttga ttgagtacgc cttcgttcgc ttgcgcctcg cccgaattgt ggcgacgacg
       61 acctacgaca ataccgcctc ggtgggcgtg atgcgcaagc tggggatgcg ggtcgagaga
      121 aacccacttg cagaaccccc gtggttgcag gtggtggggg tgctcgaggc cgttaggcgt
      181 ccctaggccc aaatcggagg tgccggtgac agatcctacg atctcgaccc tttctgaact
      241 gaagatcgtc attgatggct cttggtccga gctcgaggcc ttccttgcag gtcttactga
      301 gcagcaggct tcggttcaag acgaccaggg ttggaccgtc acggatcatg tcacccacat
      361 cgcggtgtgg gaggactccg tcgccatact ctttcggggg aagccccgcc acgaggcgct
      421 tgggattagc gagtcttact acgcccaagc ctcgttcgat gagatcaacg aacaaatcag
      481 gcagcgtatg ggccacatgc cgcttagcct agccatcgag caattacggc aagtgcatct
      541 gacgttgatg gccaacttcg atagtctgtc ggaggctgag ctaagcatgg cggttcggga
      601 cttcttttct gaggcccctc gctcagacga gaggcgagtc attgatttct tccaggacaa
      661 cacggcggac cacttcaagg agcaccttgg gtggatgcag accttgctca agagggccag
      721 ctaaccctgc gctggagccc ttcgctgcgc tcaggatgta cggacgccgc cgctacgcga
      781 ttccacgatc gaggtcactc agagacggcg accagctcat gggggttaga ccgccgctcg
      841 aaccaactat ccaaggaggt gtggagtggc tcatcctctc gttgaccaat tgaggttcac
      901 ccgctcggag tggctgcgcg gcctcgaagg catctctgat gaagatggct ctcgccattt
      961 cggcccgatg aactgcatca gctggatcgt cggccacctg gcctggcagg agcaacgata
     1021 tttcctgcag cgggcgcagg ccaggttgat cttccccgag ctcattaccg cgtatgccta
     1081 tggcgcgccc atgagcactc ctgccctgtc cgacacctcg cgcttttgga atgaggtgat
     1141 ccagacaagc gctccctacc tggacggcct cacaacggtc tctcttcagg aggagctgct
     1201 cctggaaggt aagcccgttg gccaatctgt tggatcagcc atgcgaagga tgacttacca
     1261 ttactggtac cacatcggcg aaatccaggc tattcgccag atgctcgggc atcccggctt
     1321 acccgagtac gtcggtgaca tagagaccct tgccccgtac cggccagaac aatagcctgc
     1381 gctccgtaga agcgggccac ctcctcatgg gtgcggaccc gaactcggcg cggtcccatc
     1441 atcagaggag cccatcggcg cagttgaatt cgcggccgtt agggtctgcg cgggattggt
     1501 tttggtcggg ccgaccggtt gaggtcaaac ccgtcggccc agctggcaac aaacatatgc
     1561 cttttggaca gacaccatga tccgcatctg gctgatcgga atcagtttgg cagtcacagc
     1621 cgcctgcaac cccagccagc agtcccctat ggtggacgga tctggtgcga ccccgagcgc
     1681 caccgccacc gagtccccat cggctgcgcc ggtcctttcg ccgacgccgg gcagccgtcc
     1741 agcaattgtc gagacaacga cgaccgggct gatcatcaac gggaatgtac gtcttgaaaa
     1801 cggaacgccg ttggccaacg tccagatctg ccgcaatttc gcggcataca atggaaaaac
     1861 ggtggcggtt accgatgaga ggggtcgcta cgaagcggcc cttgcgttca tccctgggat
     1921 cgaaacgatc ggggtctggc ccaagctggc gggatacacg tttgaccccc ctttctatga
     1981 ttgggtccac tattacgggc tggagacccg gacactcgac ttcgttgcca agatttcata
     2041 tgggaccccg ggtcctccgt ttcctgttga atgcgacccg taggaccagc gattgctgat
     2101 ctaagtgtgt cgttcggtat ttcgctcggg gtaggccgtt tctcggtggg tggcgttgcc
     2161 ggacgcccct cgtgaagtca ggacggggat gatcgagcgg cgtttgcggg cgcggcaagc
     2221 cactttataa tgggcccgcg cgatgaagtt gcacctcctg tacaggctga cggcgtggac
     2281 accatcagtg gagcgggagg tgcagtgctt tcatacgatc agctcgctga agccggcccc
     2341 actgtgccgg caagctggca gccggtaccc gcttcttgct gttgacccgc tacccatcgg
     2401 tgctcttccc gggaagtgcg gatggcacaa cggagccgat cgttgcgtgg ccgctggaag
     2461 agtgggtcgg tccgcagagc ttaacggcag ccaagctagg taattgacag aaaaatgacg
     2521 acccgtgtcc gccgagtgag ccgaagagga ggaatacctt gcaaagaatg agatcagctt
     2581 ccgcacaaca aggcacgcat cgcacctacg cgttggtagg attggcggcg attgctttcc
     2641 aggtcctgtc ggcctgcgcg ccgcagggcc agccgccgat tactccctcc gagacctcgg
     2701 cctcgcccgc cgcgccgacc gccgcgtcga ccgcggtccc accggcgcct tcgccgaccg
     2761 ccgacccgcg ctgccccgag ccgacggagg gcacgcagtt gctcacacac gaagaggacg
     2821 gctactgctt cctctatccc gacggactgc ttcgggtgga ttcatcccag catgcggtgt
     2881 gcctcgtccc tgaaggggac ctcctgggat gcgatagctt cgtcgccgct gtcgaagtta
     2941 ccgacgccga gggaaggacc gccgcccaga tcgccgacga gaaggtgctc agtgaaggca
     3001 gcgtcggggc cgaccgttgc accgcaccga tcgccggcat ccagaccgtg gttctacccc
     3061 aggttgccga tcaggggctt a
//
LOCUS       DATDPQ010000032         9163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2627623, whole genome shotgun sequence.
ACCESSION   DATDPQ010000032 DATDPQ010000000
VERSION     DATDPQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627623"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="iolG"
                     /locus_tag="VI520_00735"
     CDS             <1..794
                     /gene="iolG"
                     /locus_tag="VI520_00735"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HLF36437.1"
                     /translation="TDTHAGLIQQAAAAGKHVFCEKPIDFDLARIDQTLAAVQQAGVK
                     LQIGFNRRFDPSYRKVRQLVLEGRIGEPHLLRITSRDPGPPPLEYLKTSGGIFLDMTI
                     HDFDMARFLIGSEVEELYVAAAVRVDPQIGELGDLDTAVITLKFANGVIGTIDNSRQA
                     VYGYDQRAEVFGSRGMATTGNETPDRHRLADGTGIHGPLPLHFFLERYQTAYLEEMRS
                     FVECLRTGKPPEVSGADGRAPVAMGLAAQRSYRENRPVRLDEVEG"
     gene            807..1757
                     /locus_tag="VI520_00740"
     CDS             807..1757
                     /locus_tag="VI520_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLF36438.1"
                     /translation="MKIGLYTDSLAELDFEVALDWIAAQGIEAVEIGTGNFSPAPHCE
                     LQGLIRDDAARRQFLGAIERRGLVLSALNCSGNPLDPHPERRPRAQQVLSHTLQAASE
                     LGIDTVVTMSGCPGDLGGGTYPNWVTCTWQREYVELVERQWAEEIEPYWRQVSDRARV
                     LGVRIAIEMHPGQAVYNTRSLQRLREIGGADVIGANLDPSHLFFQGMEPVVVIRALGQ
                     GAVFHVHAKDTRINPQEMALNGGLDTRPMSRAGVRSWEYVTLGFGHGELFWRNFVSTL
                     RVMGYDGSLSIEHEDRLMSPKEGIQKSVDFLQGLIPRTMA"
     gene            1905..3011
                     /locus_tag="VI520_00745"
     CDS             1905..3011
                     /locus_tag="VI520_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF36439.1"
                     /translation="MKPDWKWLGLVLFGSVLVGACATPTPIVQTVEVPQSVTQIVEVE
                     RQVAVTPTPIGGQLTLYACLFDPDKLQVLFETFEALYGVEVTCLDMSSGEALERIRAE
                     KDNPQGDVLFGTTNLSHVNLAADGLLEPYKGAGWNQLPDGAIKDPDGNWTGFYYGVIG
                     FACSPARLAEINAECPDSWQDLLDPVYKGEIVIASPAASGTSYTVLSGLSQLFGGEDA
                     AFEWYHQFNENVAQYTESGSAPGKLAAAGEFAVGIAFAHDIQVQQDNGLDVLLNFPSE
                     GTPFEIGGISIIKGAKNLAAAQAWVDYVFSTAFQRYHNDVAHRIPVVPGVELAEGTFG
                     LEDVVLIEGYDPTEWAAQRDPLVARWQEEIGATR"
     gene            3054..5000
                     /locus_tag="VI520_00750"
     CDS             3054..5000
                     /locus_tag="VI520_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLF36440.1"
                     /translation="MRRVLSSFLGFPPEAARLVANTRPWPGLSQRQRQRFSLLAVLAV
                     QLTILGVVANTVLSLDVVEDRREVVGGVETIHFRRELVGDVPLLIIAALGMAMPAVSS
                     RIRREPVLLVAVQLAAVGLIVFVFWPLAQVFLEGFRAAQGATGFSLLQFERLLASPTV
                     ARASINTLLIGVISAVLSTSIGTLVAYTLTLTDIPWKRLLRVLVVLPLVSPPFVVSHA
                     FIMLFGRRGLITYDLLHITKWTIYGPDGIVLVQLISDIPLVVLILMAVFASISRDLED
                     AAEDLGGRPFHVLRTVTFPLVTPAILTAALLTFISSIADFGNPMLIGGGFQVLATQAY
                     IQLIELFDLQLGAALAMLLVIPALAAFALQHWITSRRSYVTVTGGARTGGVRKLPPLI
                     KLPLFGLSFLLAGFTVLLYGSIFVGALVETWGFDFTPSTRHLVGLVTAVPQLRNSLIV
                     SIGSGVVGGVVGIVIAWLVARQRFPGRGALDFSATLMYAIPGTVVGIGYIIAFNAAPY
                     FWTGTFFIIIVAYAYRRLPVGLRTGVAAQKQIDPALEEASLDLGASRIRTFARITFPL
                     LSRAFFAGVIYIFIRGMTDLSSAVFLNAGRTQLYTVRMFRVIITGSPSEAAAFAGLLI
                     VIILIALGVLSKITGKSFVDLFRI"
     gene            5003..6085
                     /locus_tag="VI520_00755"
     CDS             5003..6085
                     /locus_tag="VI520_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36441.1"
                     /translation="MDPTPQFATAGITLKDLSKRFGDVPAVEKVNLKVEAGTLVCLLG
                     PSGCGKTTTLRMIAGFEDPTQGQVLIGEDDITRMPPYARPTAMVFQSYALFPHMSVFE
                     NVAYGLKARRRPKAEVEQRANEAIQLMELEGNERKSPPQLSGGQQQRVALARALVIRP
                     KVLLFDEPLSNLDAQLRVRMRGEIRSLQRRLGITSVYVTHDQEEAFSIADVVALMNKG
                     KLIQLGTPRELYRQPADRFVAEFVGLSNVVSAELVESRQDGAVVRVLGQTIRSRRPPK
                     NPQGPVSVVLRPEAMAIAASGDHGVPARVLNLAYVGPIARYRVQVERYDTELTVDLSN
                     PGPDQFYEEGSEVRIQLPAEVPSLLS"
     gene            6154..6723
                     /locus_tag="VI520_00760"
     CDS             6154..6723
                     /locus_tag="VI520_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLF36442.1"
                     /translation="MAEKHILAVCFDFGDTLVDEASEVKDEAQTTLRAELIPGAAELL
                     NELNRRGYPLGLVADGRPGTYSNVLGHYGLDKFFNAWSVSEELGLLKPDRRMFVHALE
                     RLGIGPEQYGRTLMVGNRLERDVRGANGLGMISVWMDWSPRYAKLAADPSEQPHFRVR
                     EPLEVLNVIDELERRAGSGQRSDRGDPAS"
     gene            complement(6806..7624)
                     /locus_tag="VI520_00765"
     CDS             complement(6806..7624)
                     /locus_tag="VI520_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLF36443.1"
                     /translation="MTGNWKLAIQEDMLPGPTLEDRFAQAAELGLSGIEFWSATLPEQ
                     ANEIERLSGRGGVVAASINNGRRSRFLDPDLGERARALTELSEAIRLAGQIGASGVAF
                     VPHFFGPLIPDLSPYMTPLQLERSLLSAQLQELTEVAERAGVQLWVEPVNHYETHLLV
                     RLADAASIVAPLSSPRLGIVADLFHMALEEEAIDRAIVQNGPTIGHVHLADSNRRLPG
                     QGSTDFHAALAALREIGYDAWLAFECGEPGRNHDRATVYFAELPAAIRSVLGGS"
     gene            complement(7621..8388)
                     /locus_tag="VI520_00770"
     CDS             complement(7621..8388)
                     /locus_tag="VI520_00770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLF36444.1"
                     /translation="MGGPNLADHPPIRLIVLDIDGVVTQGEAQPLDLGLLGLLAGLNR
                     QARGNPEQPAVTLCSGRPAPYVEVLLQAIDGHLPAVYENGAGLYSPVGYQFTPHPDMR
                     FIDDRFQEVQERLQVGLVASGKAYFQPGKVYSLSLFAREPVATTQLRELAAAALGSLG
                     ESVDLVDSPSCLNVLPRGIDKGKGLAYLADTIGLPLERMLGVGDSDVDLPFLARVGLS
                     AAPANANPAVRQMVNFLSARPNGDGVRDILQHFRLLP"
     gene            8506..8874
                     /locus_tag="VI520_00775"
     CDS             8506..8874
                     /locus_tag="VI520_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF36445.1"
                     /translation="MAKHPIVHLELSAKEPKAAGEFYERLFGWKIETDEKLGYTQFSP
                     EDEGGVGGGFNPIDDNTPAGSVVAYVGTDDIAASLKKAESLGGKALMPKTEIPGVGWF
                     GMFMDPSGNRVGLYTTANPQ"
     gene            complement(8949..>9163)
                     /locus_tag="VI520_00780"
     CDS             complement(8949..>9163)
                     /locus_tag="VI520_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36446.1"
                     /translation="ILLGPPTPIWSGDVPSTANITGGWSGTIGDRQLGTSSSEELRFV
                     FESGAPPSGYSLTVTFDNSCTAVFNN"
BASE COUNT         1611 a         2845 c         2983 g         1724 t
ORIGIN      
        1 ccaccgacac ccacgccggg ctgatccagc aggcggctgc ggccggcaag catgtcttct
       61 gcgagaagcc gatcgacttc gacctggccc gcatcgacca gaccctggcc gcggtccagc
      121 aggcgggggt gaagctacaa atcggcttca accgtcggtt cgaccccagc taccgtaaag
      181 ttcgccagtt ggtgctggag gggcggatcg gcgagccgca tctgctgcgg atcaccagcc
      241 gggatcccgg cccacccccg ctagagtatc tcaagacctc ggggggcatc ttcctggaca
      301 tgaccatcca cgacttcgac atggcccgct tcctgatcgg gagtgaggtg gaggagctgt
      361 acgtggcggc cgccgtgcgg gtcgatccgc agatcggcga gctcggcgat ctggacaccg
      421 cggtgatcac cctcaagttc gcgaacggcg tgatcgggac catcgacaac agtcgccagg
      481 ccgtctacgg ctacgatcaa cgcgccgagg tattcggctc ccgcgggatg gccacaaccg
      541 gcaacgaaac ccccgatcgc caccgtctag cggacggcac aggcattcat ggcccgctgc
      601 ctctccattt cttccttgag cggtatcaga cagcctacct tgaagaaatg cgcagctttg
      661 tcgagtgcct gcggacgggc aagccgccgg aggttagcgg ggcggatggg cgggctccgg
      721 tggcgatggg gttggcggcg cagcgatcgt atcgggagaa ccgcccggtg cggctggacg
      781 aggttgaggg ttgaggaggg gtcgatttga aaattggcct gtacaccgat tcgctggctg
      841 agctagattt cgaagtggcg ctggattgga ttgcagcgca aggaatcgag gcggtggaga
      901 tcggcactgg gaacttctcg cctgccccgc actgcgaact gcaggggctg atccgggatg
      961 acgcggcccg tcgccagttt ctgggtgcga tcgaacggcg cgggctggtg ctgagcgcgt
     1021 tgaattgcag cggcaacccg ctcgatccgc atccggaacg ccggccgcga gcgcagcagg
     1081 tgctcagcca caccctccag gctgccagcg aactggggat cgatacggtg gtgaccatga
     1141 gcggctgccc cggcgatctg ggtggcggca cttatcccaa ttgggtgacc tgtacctggc
     1201 agcgggagta cgtggagctg gtcgagcggc agtgggcgga ggagatcgag ccctactggc
     1261 ggcaggtcag cgaccgggcc agggtgctcg gagtccggat tgccattgag atgcacccgg
     1321 gccaggcggt ctacaacacc cgcagcctgc aacgcctgcg agagatcggc ggcgccgacg
     1381 tgatcggggc caacctggat cccagccacc tcttctttca ggggatggag ccggtcgtcg
     1441 tgatccgggc gttgggccag ggcgcggtct tccatgtgca cgccaaagac acccggatca
     1501 atccgcagga gatggcgctc aatggcggcc tggacactcg cccgatgtcc cgggccggcg
     1561 tccgatcttg ggagtacgtg accctgggat tcggtcacgg ggagctgttc tggcgtaact
     1621 ttgtgagcac cttgagggtt atggggtatg atggctcgct gagcatcgag cacgaagacc
     1681 ggctcatgag cccgaaggag ggaatccaga agagcgtcga cttccttcag ggcttgattc
     1741 cccgcacgat ggcttgagga acttccggcg cgctcagctg ggctcggcag ccaatctcag
     1801 tagatcaata ggcattggga ggtgagcctg cacctgatcc ggtccccata gaaccgcaac
     1861 ggaattcaca ccgaagcttt cgatcactct aggaggagaa ttcaatgaaa cctgattgga
     1921 aatggcttgg tcttgtgctg tttgggagcg tgctggtcgg cgcctgcgcc accccgaccc
     1981 cgatcgtgca gacggtcgaa gtaccgcagt cggtcaccca gatcgtggaa gtcgagcggc
     2041 aggtcgcggt cacccccacc ccgatcggtg gccagctcac cctgtacgcc tgcctgttcg
     2101 atcccgacaa gctgcaggtg ctctttgaga ctttcgaggc gctgtacggg gtcgaggtta
     2161 cctgtctgga tatgagcagc ggcgaagcac tcgaacgtat ccgggcggag aaggacaacc
     2221 cccaagggga tgtgctcttc ggcaccacaa atctctcgca cgtcaacctg gcggccgatg
     2281 gcctcctaga accctataag ggggcaggct ggaaccagct gccggatggc gccatcaaag
     2341 acccggatgg caactggacc ggtttctact atggcgtgat cggtttcgcc tgcagccccg
     2401 cccgtctggc ggagatcaat gccgaatgcc cggactcctg gcaggatctg ctggatccgg
     2461 tttacaaggg tgagatcgtg atcgccagcc cggctgcctc cggcacctcg tacaccgtgc
     2521 tgagcggctt gtcccagctc tttggcggcg aggacgctgc cttcgagtgg tatcaccagt
     2581 tcaacgagaa tgtggcccaa tacaccgaat ccggcagcgc gcccggcaag ctggcggccg
     2641 ccggggagtt cgccgtgggg attgcctttg cccacgacat tcaggtgcag caggacaacg
     2701 gcctggacgt gctcttgaac ttcccgtcgg agggcacgcc ttttgagatc ggcggcatct
     2761 ccatcatcaa gggagccaaa aacctggcgg cggcgcaggc gtgggtggac tatgtgttct
     2821 ccaccgcctt ccaacgctac cacaatgacg ttgcacaccg gatcccggtg gtgcccggcg
     2881 tcgagctggc ggaaggaacc ttcggcctgg aagatgtggt gctgatcgaa ggctacgacc
     2941 ccaccgaatg ggctgcccag cgcgatccac tggtcgctcg ctggcaggag gagatcggcg
     3001 ctactcgata gcttgtggca tccggagggg ggcggggatt cctgcccccc tccgtgcgta
     3061 gagttctcag ttcgtttctc ggtttccccc cggaggcagc ccgcttggta gccaacacta
     3121 gaccctggcc aggattgagc cagcggcagc ggcagcgctt ctcgctgttg gcggtcttgg
     3181 cagtacagct gaccatcctc ggagtcgtcg ccaacacggt gctatccctg gatgtggttg
     3241 aggaccgccg ggaagtggtg ggcggtgtag agaccatcca tttccgccgc gagctggtgg
     3301 gcgatgttcc cttgttgatc atcgctgcct tggggatggc catgccagcc gtttcgtccc
     3361 gcatccggcg tgagccggtg ctgctggtgg cggtgcagct cgcggcggtc gggctgatcg
     3421 tcttcgtctt ctggccgctg gcacaagtgt tcctggaagg ctttcgggct gcgcagggag
     3481 cgaccggatt ctcgttgctc caattcgaga ggctgctggc ttcccccaca gtggcacggg
     3541 cctcgatcaa taccctgctc atcggcgtca tttctgcggt gctgtccacc agcatcggga
     3601 cgctggtggc ctacaccttg accctgaccg acatcccctg gaagcggctg ctgcgcgtgc
     3661 tggtggtcct gccgctggtc tcaccgccct tcgtggtgag ccatgccttc atcatgctct
     3721 tcggacggcg aggcctgatc acctacgacc tgctccacat caccaagtgg accatctatg
     3781 gaccggatgg gattgtgcta gtacagctca tcagtgacat ccccctggtg gtcctgatcc
     3841 tgatggccgt gtttgcctcc atcagccgcg acctggaaga cgcagcggag gatctcggcg
     3901 ggcgaccctt ccatgtgttg cgcaccgtga ccttcccgct ggtgactccg gccatcctca
     3961 ccgcggccct gcttaccttc atttcatcca tcgcggactt tggcaacccc atgctcatag
     4021 gcggtggttt tcaggtgctg gctacacagg cctacatcca gctgattgag ctgttcgacc
     4081 tgcagctcgg cgcggcgttg gccatgctct tagtcatccc ggctctggcg gcctttgcct
     4141 tgcaacactg gatcaccagc cgccggtcgt acgtcactgt gactgggggg gccagaaccg
     4201 gcggcgtccg caagctgccg ccgctgatta agctcccgct cttcggcctg agcttcctcc
     4261 tggctgggtt caccgtcctg ctgtatgggt cgatcttcgt gggggccctg gtggaaacct
     4321 ggggtttcga cttcaccccg agcacccgcc atctggtcgg tctagtcacc gcagtccccc
     4381 agctgcgcaa ttccctgatc gtgtccatcg ggtccggagt tgtaggcggc gtggtcggga
     4441 ttgtcatcgc ctggctggtc gcccgtcagc gatttccagg ccgcggcgcg ctggatttct
     4501 ccgcaacgct catgtatgcc atccccggca cagttgttgg gattggctac atcatcgcct
     4561 tcaacgctgc cccttatttc tggaccggaa ctttcttcat catcatcgtc gcctacgcct
     4621 accgacggct cccggtcggc ctgcggaccg gcgtggcggc gcagaaacaa atcgatcccg
     4681 ccctcgagga ggcctcgctc gacctgggcg cctcccgcat ccgcaccttt gcccgaatca
     4741 ccttcccgtt gctcagccgg gccttcttcg ccggggtcat ctacatcttc atccggggca
     4801 tgaccgatct gagctcggcg gtcttcttga acgcgggccg cacgcagctc tacaccgtcc
     4861 gcatgtttcg ggtgatcatt accggctcgc ccagcgaagc ggccgccttt gccggtttgt
     4921 tgatcgtaat catcttgatc gcgctcgggg tgctgagcaa gatcaccggc aagagttttg
     4981 tggatctctt caggatctag gcatggaccc gaccccgcag ttcgcaaccg ccggcatcac
     5041 cttgaaggat ctcagcaaac gcttcggcga tgtgccggcg gtggagaagg tcaacttgaa
     5101 ggtggaggcc ggcaccctag tgtgcctgct gggccctagc ggttgcggca agaccactac
     5161 tttgcggatg atcgccggtt tcgaggaccc cacccagggg caagttctca ttggcgagga
     5221 cgacatcacc aggatgcctc cctatgctcg ccccaccgcc atggtttttc agagctacgc
     5281 gttatttccc cacatgagcg tgttcgagaa cgtggcctat ggactgaagg cgcgccggag
     5341 gccgaaggcg gaggtcgagc agcgggcaaa tgaggccatc cagctcatgg agctggaggg
     5401 caacgaacgc aagtcgccgc cgcagctctc cggtgggcag cagcagcggg tggcgctggc
     5461 ccgggcgctg gtgatccggc ccaaggtgct gctcttcgac gaaccgctct ccaatctgga
     5521 tgcacagctg cgggtgcgca tgcggggcga gattcgcagc ctgcagcgcc ggctcgggat
     5581 caccagcgtg tacgtcaccc acgatcagga ggaggccttc agcatcgccg acgtcgtggc
     5641 gctgatgaac aagggcaagc tgatccagct gggcacaccc cgcgagctgt accgccagcc
     5701 ggcggaccgg tttgtggccg agtttgtcgg cctctcgaat gtcgtgtcag ccgagctggt
     5761 cgagtcgcgc caggatggag cggtggtccg ggtcctcggg cagacgattc gcagccgccg
     5821 gccgcccaag aacccacagg ggccggtgtc ggtcgtgctt cggccggagg ccatggcgat
     5881 tgccgctagt ggcgatcacg gcgttccggc ccgggtgctt aacctggcct acgtgggtcc
     5941 gatcgcacgc taccgggttc aggttgaacg ctacgacacc gagctcacgg tggacctgtc
     6001 caacccgggc cccgatcaat tctatgaaga gggctcggag gtgaggatcc agctccctgc
     6061 ggaagtccct tccctgctga gctgagctgg cggccggccg aggcgggctg aataggaggt
     6121 ggatcggcgc ggtctggacg cgccggatcg tttatggccg agaagcacat cctcgcagtt
     6181 tgtttcgatt ttggcgatac gcttgtcgat gaggccagcg aggtcaagga cgaggcgcag
     6241 acgaccctgc gagcggagct catcccgggg gccgcggagt tgctgaacga gctgaaccga
     6301 cgcggctatc ccttggggct ggtagcggac ggccggccgg gcacctattc caatgttctc
     6361 ggccactacg gcctggacaa gtttttcaac gcctggtcgg tctctgaaga gttggggctg
     6421 ttgaagcccg accggcggat gttcgtccac gccctggagc ggcttgggat cggcccggag
     6481 cagtatggcc ggaccttgat ggttgggaac cggctggagc gggacgttcg cggtgcgaac
     6541 gggctgggga tgatcagtgt gtggatggac tggtcgccgc gctatgccaa actggcagcc
     6601 gatccgagcg agcagcctca ctttcgggtg cgcgaaccgc ttgaggtgct caacgtgatc
     6661 gacgagcttg aacgccgggc agggtcgggc cagcggtccg accgcggcga tccagcctcc
     6721 tgacactgat ctggaactga agccggcgcg accggggtcc aagctcgttc cctcgcgagc
     6781 aagcgcgcct cgcgaaggaa gcaggctagc ttccgccgag caccgaccgg atcgctgcgg
     6841 ggagctccgc gaagtagaca gtcgcccggt catgattccg gcccggctct ccgcactcga
     6901 aggccagcca agcgtcgtac ccgatctcgc gcaaggcggc tagagctgcg tggaaatcgg
     6961 tcgacccctg gccgggcagc cggcggttgc tatccgccag atggacgtga ccgatggtgg
     7021 ggccattctg cacgatcgct ctgtcaatgg cctcctcctc gagggccata tggaataggt
     7081 cggcgacgat ccccagccgc ggtgagctga gcggcgcgac gatcgaggct gcgtcggcca
     7141 atcgcaccaa aaggtgggtc tcatagtgat tcaccggctc gacccagagc tgaacgccgg
     7201 cccgctcggc cacctccgtc agctcttgca actgcgcgct gagcagcgat cgttccaatt
     7261 gcagtggggt catgtagggc gacaggtcgg gaatcagcgg cccgaagaag tgaggcacga
     7321 aggccacgcc ggaggcgccg atctggccgg ccagtcgaat cgcctcgctt agttcggtaa
     7381 gcgcccgggc ccgttcgccc aaatcggggt ccaagaagcg agagcggcgg ccgttgttga
     7441 tcgaggcggc gacgacgccg cctctgccgc tcagccgctc gatctcattc gcctgttcgg
     7501 gcagcgtggc gctccagaat tcgatcccgc tcaagccgag ttcggccgcc tgggcaaacc
     7561 ggtcttccag cgtcggtccg gggagcatgt cctcctggat ggcgagtttc caattgccgg
     7621 tcatggcagc agcctgaaat gctgcaggat gtcccgcact ccgtcgccgt tggggcgggc
     7681 cgagaggaaa ttcaccattt ggcggacggc ggggtttgca ttggccggcg cggcgctcag
     7741 gccgactcga gccaggaatg ggaggtccac atcggaatct ccgaccccga gcatacgttc
     7801 cagcggcagg ccgattgtat cggccaggta ggcgaggccc ttgcccttgt cgatgccccg
     7861 cggcagcaca ttaagacagg aaggcgaatc caccaaatcg accgactcgc caagtgagcc
     7921 aagcgccgca gcggccagct cgcgaagctg ggtcgttgcg accggttccc gggcgaacag
     7981 gctgaggctg tacaccttgc cgggttggaa gtacgccttg ccgcttgcga ccagcccgac
     8041 ctgcagccgc tcctggacct cctggaaacg atcgtcgatg aaccgcatgt cggggtgggg
     8101 cgtaaactga tagccgaccg gactatataa accggccccg ttttcataga cggctggcag
     8161 atgtccatcg atcgcttgca acagcacttc gacatacggc gccggccggc cgctgcagag
     8221 ggtgaccgcc ggctgctcgg gattgccgcg ggcctgtcgg ttcaatccgg ccaatagccc
     8281 gagcaggccc aggtccagcg gctgggcctc gccctgggta acgacgccgt cgatgtcgag
     8341 gacgatcagc cggatcggtg gatggtctgc caggttgggt cctcccattt ctggaattgt
     8401 agccagcgac cgatcgcctg acaatcgaag gagggattaa aattgactag atagaacagt
     8461 tgtgctaatc ttagacatcc catatcatgg ttgaaggagg agaagatggc gaagcacccg
     8521 atcgtgcact tggaactctc ggccaaggaa cccaaggccg ccggcgagtt ctacgagagg
     8581 ctattcggct ggaagatcga gacggacgag aagctcggct acacccagtt ctcacccgaa
     8641 gatgagggtg gggttggagg cggcttcaat ccaatcgacg ataacacgcc ggcggggagc
     8701 gtggtggcct acgtcggcac cgacgacatt gcggcctcac tgaagaaggc cgaatcgctc
     8761 ggaggcaaag ccctcatgcc gaagactgag attccggggg tcggctggtt cgggatgttc
     8821 atggatccct ctggcaaccg ggtcggactg tacactaccg cgaacccgca gtaacccgac
     8881 gcaccaagac tcaaacgggc gacctctgga gcaccagatg gtcgcccact gcctaatccg
     8941 atggtctatt agttgttgaa aactgccgtg caactgttgt cgaaggtgac tgtcagcgag
     9001 tatcccgaag ggggcgcgcc actctcgaac acgaatctca gttcctccga actgcttgtt
     9061 ccgagctgac ggtctccgat ggtccccgac caacccccgg tgatgttcgc cgtcgaaggc
     9121 acatccccac tccagatcgg cgtgggcggg cccagcaata tcc
//
LOCUS       DATDPQ010000033         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1582006, whole genome shotgun sequence.
ACCESSION   DATDPQ010000033 DATDPQ010000000
VERSION     DATDPQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582006"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VI520_00785"
     CDS             <1..696
                     /locus_tag="VI520_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLF36447.1"
                     /translation="NPLREIAALAKQHAVPIVVDAVSSLAGASLEMDDWGIDICVSAT
                     QKGLGAPPGLGPIAISDRAWEIMDSKPARNHGWYLNLQTWRKFATEWGAWHPHPITMA
                     TNNVMALRASLRTLLAEGIDARIDRYTRLALRLRSGVRRLGLEPLTSDERLAPVLTAI
                     YGPDGVRSSDIVKYLREEHDLRISGGLGEGLAERVFRVGHMGPRVNEQDIDLVLSGLE
                     GFLQHRGILQTAP"
     gene            703..>2921
                     /locus_tag="VI520_00790"
     CDS             703..>2921
                     /locus_tag="VI520_00790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLF36448.1"
                     /translation="MEDLSGKTLGKYQIHSPLGQGGMAAVYKAFQPGVERFVALKILP
                     RQYASDPEFTGRFEQEAKVIAQLQHPHILPVHDFGQADGYTFLVMPLVETGTLADLLK
                     GFALPPGRIRTIISQIGDALDYAHSQGLIHRDVKPSNILIDRRGNCLLTDFGIAKMVQ
                     GTKHFTATGGILGTPTYMSPEQGMGEKLTQTSDIYSLGVVLYEMATGKVPFDAETPLA
                     VVIKHMNDPLPLPSKLKPEISRTLETVIMKALAKAPGDRFPTAADLVRAVRTAIPESI
                     GEAAEVQISTVPRKELATAEELALGDSAELAAATPTPIPLEPPPAPVEPARKQPGSLR
                     RLGPWVVAGGLALLGLCGLGSYGLIRVSGLFPSESESQALETSLALEAQASTSESQAA
                     TSQAEVDLAATATVAAVVAAAQTASVELAAIGTSTLEAHPTETSPLHAASLAELAAAH
                     WPLVLAESFDNNSAGWTPFSNFSDDFGTRTFVFASGSYRWTVQPDRDVNLHDTPEMNA
                     LTDFYAAVSVRQSDGPDSADYGLAFRVISSEGLDSFYSFSISDSQQYSWQLLQAGEWT
                     TLIPWTTSEAIVAGGTNRVAVLAEGEHFAFFVNDQLVDEGENSGLIQGRAGILVDMFD
                     PGVSGEFVFDDFEIRQPWTAAVVEPFEVRTGLFDTGNSSSQDLSESLSIGGGKYHWTI
                     DCEDAEFGCISSSYLKTLEDTTDFQLSVEARRIEGPLGGQYGLRFRDDGLSYFEFLIA
                     "
BASE COUNT          556 a          951 c          891 g          523 t
ORIGIN      
        1 aatccgctgc gcgaaatcgc tgctctcgcc aagcagcacg cggttccgat cgtcgtggac
       61 gcagtgtcct cgctcgccgg cgcatcgctg gagatggacg actggggaat cgacatctgc
      121 gtgagtgcca cccagaaggg cctgggcgcg ccgccggggc tcggcccgat cgcgatcagc
      181 gaccgggcct gggagatcat ggactcgaaa ccggcccgca accacggctg gtacctcaat
      241 ctgcaaacct ggcgcaaatt cgccaccgag tggggcgcct ggcatccaca tccgatcacg
      301 atggccacca acaacgtgat ggcgctgcga gctagtctcc ggacactgct ggctgaagga
      361 atcgatgccc ggatcgaccg ctatacccgg ctggcgctgc ggcttcgcag cggcgtccgg
      421 cggctcgggc ttgagcctct cacctcagac gagcggctag caccggtgct caccgcgatc
      481 tacggcccgg acggcgtgcg cagcagcgac atcgtcaagt acctgcgcga ggagcacgac
      541 ctgcggatct caggcggcct gggtgaaggt ttggcggaga gagtctttcg ggtcgggcac
      601 atgggacccc gggtcaacga acaggatatc gacctggtcc tttccgggct ggagggattc
      661 cttcagcacc ggggcatact ccagaccgca ccttgatctg cagtggaaga cctctccggt
      721 aagaccctcg gtaaatacca gatccactct ccgctcgggc aaggcgggat ggcagccgtg
      781 tacaaggcct tccagccggg agtcgagcgc tttgttgcgc tcaagatcct tcccaggcaa
      841 tacgcaagcg accctgaatt caccggccgt ttcgagcaag aagccaaggt catcgcacag
      901 ctgcagcacc cccatatcct tccagttcat gacttcggcc aggccgatgg ctacacattc
      961 ctggtcatgc cgttggtgga gactggcacg ctggccgatc tgctcaaggg gtttgctttg
     1021 ccgccgggga ggatccgcac catcatctcc cagatcggcg acgctctgga ttatgcccac
     1081 tcccaaggcc tgatccatcg ggacgtcaaa cccagcaaca tcctgatcga ccgccgcggc
     1141 aactgcttgc tcaccgactt cggcatcgcc aagatggttc agggcaccaa acatttcacc
     1201 gccaccggcg ggattctcgg taccccgacc tatatgtcgc ccgaacaagg gatgggggag
     1261 aagctcaccc agaccagcga tatctattcc ctcggggttg tgctctacga aatggccacc
     1321 ggaaaagtac ccttcgacgc cgaaaccccg ctggcggtgg tcatcaagca catgaacgac
     1381 ccgctcccgc tgccgagcaa gcttaagccg gaaatttccc gaacgctgga gacggtgatc
     1441 atgaaggctt tggccaaggc gccgggggat cgcttcccga cggctgccga tctggtgcgc
     1501 gccgtgcgca cggccatccc ggagtcgatt ggcgaagcgg cagaagtcca gatctcaacc
     1561 gtaccccgca aggagctcgc aaccgcggag gaactggccc tcggcgattc ggccgaactg
     1621 gcggctgcaa ccccaacccc gataccgctc gagccacccc ctgcaccggt cgaaccagct
     1681 cgcaagcagc caggcagcct gcggcgactc ggaccttggg tcgtggccgg cgggctggca
     1741 ttgctcggac tctgtggcct cggtagctac ggcttgatcc gggtctccgg actgttcccc
     1801 tccgagtccg agagtcaagc cctagagacc tcgctcgcgc tcgaggctca ggcgtccacc
     1861 agcgagagcc aagccgcaac cagccaggcc gaggtagact tggccgccac cgccaccgtg
     1921 gccgcggtcg tcgcggccgc acaaacggcc tcagtcgagc tcgctgcgat cgggacctcg
     1981 actttagaag cccaccccac cgagaccagc cccctgcacg ctgcctcact ggcagagctt
     2041 gcggccgccc actggccatt ggtgttggcc gaatccttcg acaacaacag cgcgggctgg
     2101 actccattct ccaacttcag cgacgatttt ggtactcgga ccttcgtctt cgcgagtggc
     2161 agctaccgct ggacggtcca gcctgatcgc gacgtcaatc tgcacgacac tccggaaatg
     2221 aacgccctca ccgatttcta tgccgcagtc tcagtccgcc agtcggacgg gccggacagc
     2281 gccgactacg ggctcgcctt ccgggtaatc tcctccgagg gcctggacag cttctatagt
     2341 ttcagtatca gcgatagcca gcagtactcc tggcagctgt tgcaagctgg ggagtggacg
     2401 acgttgatcc cgtggaccac gagcgaggcc atcgttgcgg gcgggaccaa ccgagttgca
     2461 gtgctcgcgg agggagagca cttcgcgttc ttcgtgaacg accagttggt cgatgagggc
     2521 gagaattcgg gtttaatcca agggcgggcc gggatcctgg tggacatgtt cgatccgggg
     2581 gtctcgggcg agttcgtgtt cgacgatttc gagatccgtc agccgtggac cgcggcggtc
     2641 gtggagccgt tcgaagtgcg taccggattg ttcgacaccg ggaatagctc ctcacaagac
     2701 ctgagcgagt cgctctcgat tggcggcggc aagtatcatt ggaccatcga ctgcgaggac
     2761 gccgaattcg gctgtatcag ctcctcctat ctgaagaccc tcgaggacac caccgatttt
     2821 cagctgagcg tcgaggctcg ccgaatcgag gggccgctcg gtggccaata cggacttcga
     2881 tttcgggatg acgggctgag ctacttcgag ttcttgattg c
//
LOCUS       DATDPQ010000034         4846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2105042, whole genome shotgun sequence.
ACCESSION   DATDPQ010000034 DATDPQ010000000
VERSION     DATDPQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105042"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..3625
                     /locus_tag="VI520_00795"
     CDS             23..3625
                     /locus_tag="VI520_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HLF36449.1"
                     /translation="MVLILVTVGSLALASLRSREAQVNQSLALAAQSRLALQADNLDL
                     ALALAIEAIRIPDPPGEVQVALSEAAYTPGTVKVYLGHSAPVRSVAVSPDGRYGLSGD
                     DQGVIFLWDLETGEALRRLEGHQGPVSGLAFTPDGRRALSSSHDKAILYWDLEMGEVI
                     HRLEGHRASINSVAVSPDGRTAASSSGTSLWTEPFSSEDNTVRLWDLLNGAEVQRFDF
                     FTDGVTGVDFTADGRGLAVSTLSDGFLLLEVETGKAVVHRSPEINYLLQAQAVKTSPD
                     GKFALTSNAFDPINNQYKILIWDIRTAEVSQLGEHNGKITGLDIRPDGKRALSSSIGI
                     VEYDLESREQVNRFNIGANSVAYLPDGRSALAGSEDGSLRLLALASGAEIGRLPAGID
                     FIRGAAYDPAGQAVITDDSALLSRWDLETGEKIWSETFSGVFGEIALSADGRRILASD
                     GVATASLWDGASGELLGRLESDGSFVGHDSNGLVTSVALHPSGQFGLSGAEGQGTNLI
                     YWDLESGSPVWLFDTNNLDILGVAVSPDGRTALSADSEGAVSWWDLETGELLRRLEGH
                     TNRAWSVAFVDQHTAISASEDSTMILWNLESGTAIHNFLGHAAGIIRVALSPDKRLAL
                     SASRDRTAILWDVQTGEALRRYAGHTGHLMAVAWAPDGREAISGGADYQVIRWRIDAE
                     LDTLLEWIAGNRYLRPLTCDERATYKVEPLCPEGRLAEAGEPGSATIPVTVSGAALLP
                     PLPIPTPLPTDSSPALARVEEAGRAALGVNPGSFPTGGGEVWEYGGESGERLSVRVAA
                     EEPADMIGDPERQRQNGLLDPTLAIYSPDGSLLAQADDLENGLATDAYLESITLPQSG
                     IYHIEVRSFRGQTGGDYRLVLAEPRPLVFKAKVPGMAGLAIHPNGERALVGMGYSIFW
                     DSPPAEDQRIWVWDLRTGEVLRRLEGHTNTPADIAISPDGRRAISAGPEGLAILWDLE
                     SGAELLRIDNQGEVFVDVQFHPDGRTALTASTDTTLALWDLASGEVIRRFEGHEDWVN
                     DLALSSDGKTVYSTSWDNTVRVWNVGTGELLATYRPFEDGYTNGLAASPDGEYLLVGR
                     DWYPYDPTLGYDGVMALLDADTGETLRSLEGHTAMVGALAFSPDGRYALSGSRDQTVR
                     LWEANTGEQLKVFYGHAGEAWNVAFSPDGLTGYSTGSDGSLRVWDLREFVGARE"
     gene            3918..>4846
                     /locus_tag="VI520_00800"
     CDS             3918..>4846
                     /locus_tag="VI520_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF36450.1"
                     /translation="MLELPSGTITFLFSDIEGSTPLWERSPRAMQAALACHNSILQNS
                     IERHNGQVFKVIGDAFQAAFTLPAQAVAAALEAQRALAAEAWSESGPIRVRMGIHSGP
                     AEVGEGDYLASHTLNRVARITSAGHGGQVLLSAAVADHVRGLLPEDVSLKDLGEHFLK
                     GLSHPERIFQLLAPELRPDFPPLVTRITPRGYKLLEQIGAGGFGAVYRALQPEVGREV
                     AIKVILPQFANDPEFIRRFETEAQLVARLEHPHIVPLYDYWREPDSAFLIMRLLRGGS
                     LENVLERGPLNLAQAAELLEQLAGALTVAHREGV"
BASE COUNT          910 a         1421 c         1592 g          923 t
ORIGIN      
        1 caatgtactg aggactctgg tcgtggtttt gatcctggtg acggtcggtt ccctggcact
       61 ggcatccctg cgctcgcgag aagcgcaggt caaccagagc ctggcgctgg cggcccaatc
      121 gcgcctggcg ctgcaggcgg ataacctgga cctggcgctg gcgttggcta ttgaggccat
      181 ccgtatcccc gatccacccg gcgaggtgca ggtggccctc tccgaggcgg cctacactcc
      241 aggcacggtc aaggtttacc tcggccattc ggccccggta aggagcgtag cggtcagccc
      301 ggacgggcgc tacgggctgt cgggagatga tcagggggtg atcttcctgt gggacctgga
      361 aaccggtgag gcgctgcgcc ggctcgaagg tcatcaaggg ccggtctccg gcctggcctt
      421 tacgccggac gggcgccgcg ccctctccag ctcgcatgat aaagctatcc tttattggga
      481 tcttgaaatg ggggaggtca ttcaccgtct agagggtcat cgcgccagta tcaacagcgt
      541 ggcggtgagc ccagacggcc gcaccgctgc ctccagctcg ggcacgagtc tctggaccga
      601 acccttttct tctgaagata atactgtccg cctgtgggat ttactgaatg gagcggaggt
      661 gcagcgcttt gatttcttca ccgatggcgt caccggggtc gatttcaccg cggatgggcg
      721 aggactggcc gtctccacac tttcggacgg cttcctcctc ctggaggtgg aaaccggaaa
      781 agcggtcgta caccgaagcc cggaaatcaa ttatctcctt caagctcagg ctgtcaagac
      841 cagccccgac ggaaaattcg ctttgacgtc taatgcattc gatcctatta ataaccaata
      901 taagatcctg atctgggata tacgcactgc agaagtgagc cagctggggg agcataatgg
      961 caagattacg ggattggaca tcaggccaga cggaaaacgc gcccttagta gttcgatcgg
     1021 gattgtcgaa tacgatttgg aatcccgcga gcaggttaat cgtttcaaca ttggcgcaaa
     1081 ttctgtcgct tacctcccag acgggcgcag cgcgctggcc ggttctgagg atggatcgct
     1141 gcgcttgctg gcgctcgcat caggcgccga gatcgggcgt ttgcctgccg gcattgactt
     1201 catccgagga gcggcttatg acccggccgg ccaagctgtc ataacggatg attccgcttt
     1261 gctctcccgc tgggatctgg aaaccggcga aaaaatctgg agcgaaacct ttagcggcgt
     1321 ctttggggag atcgcgctgt ccgcggacgg aaggcgcata ttggccagcg atggcgttgc
     1381 aacggcctcc ctttgggatg gggcaagcgg cgaattgctc gggcgactgg agagtgacgg
     1441 cagcttcgtg ggccacgaca gcaatggttt ggtgacgtct gtagcccttc acccgagcgg
     1501 ccagttcggg ctgtcggggg cagaggggca gggcacaaac ttgatctact gggatctcga
     1561 gagcggcagt ccggtctggc tgttcgatac gaataatttg gacattttgg gagtggcggt
     1621 cagcccggat ggccgcacgg cgctttccgc cgattctgaa ggcgcggtca gctggtggga
     1681 tctggagacc ggggagttgc tccgccggct ggaagggcac accaatcggg cctggagtgt
     1741 ggctttcgta gaccagcata cagccatctc cgcctcggaa gacagcacca tgatcctatg
     1801 gaacctggaa agcggcacgg cgatccacaa cttcttgggg catgccgccg ggataatacg
     1861 ggtggccctc agcccggata aacgcctggc gctctccgct tcgcgtgacc ggactgccat
     1921 cctatgggat gttcaaacag gcgaagcgct gcgccgctac gccgggcata ccggtcatct
     1981 gatggcggtg gcctgggcac cggacgggag ggaagcgatt tccggaggcg cggactacca
     2041 ggtcattcgc tggaggattg atgcggaact ggacactttg ctggagtgga tcgcaggcaa
     2101 ccgctactta cgcccgctga cctgcgacga gcgagctacc tacaaagtcg aaccattatg
     2161 ccctgagggg aggctggccg aggctgggga gccggggagc gcaacaatcc ccgtcacagt
     2221 gtcgggggct gccctcctgc ccccgctgcc aatacccacc ccactgccaa ctgattcgtc
     2281 tccggcgctc gcacgagttg aggaggctgg ccgagcagct ttgggtgtca acccgggttc
     2341 gtttccaact ggcggcggag aggtgtggga atatggtgga gagtccgggg aacgactgtc
     2401 ggttcgcgtg gccgcggagg aaccggcgga catgatcggc gatcctgagc gccagcgcca
     2461 gaacgggctg ctggatccca ccctggctat ttattctccg gacggcagcc tgctggcgca
     2521 ggccgacgac ctggaaaacg gactcgctac tgacgcctat ctcgaatcaa ttacgctccc
     2581 gcagtccggt atctaccaca tcgaagttcg cagtttccgc ggccagactg gcggcgatta
     2641 ccgcctggtg ctggcggagc cgcgcccttt ggtcttcaaa gccaaggtac cggggatggc
     2701 cggcctggcc atccatccaa acggcgagag ggcactggta gggatgggat actcgatctt
     2761 ttgggattct ccgccggctg aagaccagcg catctgggtt tgggatttga gaaccgggga
     2821 agtgcttcgg cggctggagg gtcataccaa cacgcctgct gacattgcga tcagccccga
     2881 cggccggcga gccatcagcg ccggcccaga agggttggcg atcctgtggg atctggaaag
     2941 cggggctgag ctgctcagaa tcgataacca gggagaggtt ttcgttgacg tccagttcca
     3001 cccggatggc cggacggcgc tgaccgcttc caccgatacc accctcgcct tgtgggatct
     3061 tgccagcggg gaagtgatcc gccgcttcga ggggcatgaa gactgggtga atgacctggc
     3121 gctatcatct gacggaaaaa ccgtttattc aacatcctgg gataacactg tgcgggtgtg
     3181 gaatgtgggc acaggagaac tgctcgcaac ctaccggccg ttcgaggatg gctatacaaa
     3241 cggcctggct gccagccccg atggggagta tctcctggtg gggagagact ggtatcccta
     3301 tgatcctact ctgggatacg atggtgtgat ggccctgttg gacgcggata caggggaaac
     3361 cctgcgcagt ctggaaggcc acacggcgat ggtcggggcc ctcgccttca gtccggacgg
     3421 ccggtatgct ctgtccggct cgcgcgacca gacggtgcgc ctgtgggagg cgaatacggg
     3481 tgagcagctg aaggttttct acggtcacgc cggcgaagcc tggaacgtgg ccttcagccc
     3541 ggatggcctg accggctact cgaccggatc tgatgggagt ttgcgggttt gggatctgag
     3601 ggagtttgtt ggggctaggg aataagggat aggggatagg gggaatccgc gaatttgcgc
     3661 gaattggacg cgaatgagtg cgagggtgaa aagtggacgg cggcggagtg cgccgggcag
     3721 caaagagcgc tgctaagcgg attagcggat aagagaatgg cgaattacga actgcgaatt
     3781 gcggaggaag gcaggaaaaa ggtaggattt gtcataattc gggtgtataa tcgacgccag
     3841 ataagtcctc ttcccagcct tcgtcccggc cgaattatcg cctgcgcctt tcgtccagtt
     3901 ttgccctgcg aggcctgatg ctcgaactgc cgagcggcac catcaccttc ctcttcagcg
     3961 acatcgaagg cagcacgccc ttgtgggagc gcagcccgcg ggccatgcag gccgccctgg
     4021 catgccacaa ctccatcctg cagaattcaa ttgagagaca taatggccag gtattcaagg
     4081 tcatcggcga cgccttccag gcggctttca cgctgcctgc gcaggccgtc gcggcggcgc
     4141 tggaggcgca gcgggcgctg gcggctgagg cctggagcga gagcgggccg atccgggtgc
     4201 gcatgggcat ccactccggc ccggcagaag tgggggaggg cgattacctg gccagccata
     4261 cgctcaaccg ggtggcccgg atcacctcgg cgggacatgg cggacaggtg ctgctctcgg
     4321 ccgctgtcgc tgaccatgtg cgcggcctgc tgcccgagga cgtctccctc aaagacctgg
     4381 gcgagcactt cctcaaaggc ttgtcccacc ccgagcgcat cttccagctc ctggctcccg
     4441 agctgcgccc cgacttccct cccctggtca cccgcataac cccgcgtggc tacaaactgc
     4501 tcgagcagat cggcgccggc ggctttggcg ccgtctaccg cgccttgcag cccgaggtcg
     4561 gccgcgaggt ggccatcaaa gtcatcctgc cccagttcgc caacgacccc gagttcatcc
     4621 gccgcttcga aaccgaagcc cagctggtcg cccgcctgga gcacccgcac atcgtgcccc
     4681 tctatgacta ttggcgcgag ccggacagcg ccttcctgat catgcgcctg ctgcgcggcg
     4741 gcagcctgga gaacgttctc gagcgtggac ccttaaatct ggcccaggcg gcagagctgc
     4801 tggagcagct ggcaggcgcc ctgaccgtgg cccaccgcga aggggt
//
LOCUS       DATDPQ010000035         3695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3676697, whole genome shotgun sequence.
ACCESSION   DATDPQ010000035 DATDPQ010000000
VERSION     DATDPQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3676697"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VI520_00805"
     CDS             <1..556
                     /locus_tag="VI520_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLF36451.1"
                     /translation="GFADKKAEKLLQTIEGSKARPLSRLISALGIRGVGETVAADFAR
                     HFRNLDALAGATQQDLEAIDGIGPSTGAEVMEWFGRARNRKLLAKLKASEVWPKEAAK
                     KAGPGPLAGLTFVLTGTLPDMSRDEAKARIEAAGGKVLSSVSAKTNFLVAGEAAGSKL
                     TKAQELGVKVVDQAGLLALIKKRG"
     gene            complement(570..1913)
                     /locus_tag="VI520_00810"
     CDS             complement(570..1913)
                     /locus_tag="VI520_00810"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HLF36452.1"
                     /translation="MSTAVRCPNCRNPIQAQILQLVDVGQNPAAKARLLSGTLNHLHC
                     PVCGYEGQLATPIVYHDPAKELLLTYLPVELGIAKNEQERLVGRLINQAIDGLPAEQR
                     KGYLLQPQAVLTAQGLVERVLQADGITREELEAQRAKLRLFEDLLRTTPETLAAFVQQ
                     HDAELDERFFQLGSLSLQTAPDDRSRQAASQRLEAALSLSLFGKELAAQEAEVRSAAE
                     SLRAAGDSLTREKLLELVVEAPSPDRVRALATLARPGLDYGFFQLLSERIETVDPERG
                     ERLTKLRSSLLEITEQIDRAQEARVAQASGLLASLVNSQDLEAAVAASLPLLDDLFLN
                     VLQANIRVARERSDSKVLERLRQIDRLLAAAIQEALPVSLQLAQRVIDAETEALAQAL
                     LEESATAVDGDLLDALLSAAQRFEQLGETERAARVARLHRHALRLSMRAKMAPPL"
     gene            complement(1910..3466)
                     /locus_tag="VI520_00815"
     CDS             complement(1910..3466)
                     /locus_tag="VI520_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36453.1"
                     /translation="MLPDFRVRQRDYLLEISRVLTEELDLEKVLERIVRLSAELLSSS
                     AGLIALREQEGWRVTTTYGINPDFLKFLDPLLADIPEHDDPARFALPEVNRRLQRITQ
                     AASMGLLTGVGLPLIAREEVVGVIFIFRSYRGVFSREDRALLGAFASQAAIAVSNARL
                     YTQVADQRRHLDGVLESAADGIFILDPSYRFTRFNRACARLTGYSADFVIGKDHSEII
                     RWASREPGLTLEDAGAGGWPLSSTAELYVEGDLLRQDNGTSSLGITYAPTYSGDGRLL
                     GVVANLRDITKFREAEELKSTFISIISHELRTPVALIKGYVGTLRRDDAAWEPSVVRD
                     SLAVIEEESDRLADLIDDLLEASRLQSGTPALSLSDVDLAEIARELAERFRTQSNLHS
                     FEVQLPKGFPIVRADEERLTQVVSNLLSNAIKYASAGGVVQIQGRVAPKEVVLCVQDA
                     GPGISPADRTRVFERFFRSAETARTTQGVGLGLFLAKAVVEAHGGRIWVDEKVERGAR
                     ICFSIPRERE"
     gene            complement(3471..>3695)
                     /locus_tag="VI520_00820"
     CDS             complement(3471..>3695)
                     /locus_tag="VI520_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36454.1"
                     /translation="GLMVGAELVLDHGSKRPDHELRERVLQEAFDRGLLLLGAGESAI
                     RVVPALSIDQGLLDEGLRILEASLSAAEGR"
BASE COUNT          732 a         1130 c         1182 g          651 t
ORIGIN      
        1 aggatttgcg gacaagaaag cagagaagtt gctgcaaacg atcgagggct caaaggcgcg
       61 gccgctcagc cgcctcatca gcgcgctagg gatccgggga gtgggagaaa cggtcgcggc
      121 cgatttcgct cggcactttc gcaatctgga cgccctcgct ggtgcaacgc agcaagacct
      181 ggaggcgatc gatggaatcg gtccgagcac cggagcggaa gtcatggagt ggtttgggcg
      241 agcccgcaat cggaaactgc tcgccaagct gaaggcctcc gaggtgtggc cgaaagaagc
      301 ggcgaagaag gccgggcccg gaccactggc cggtttgacg ttcgtcctga ccggaacgct
      361 gccggatatg tctcgggacg aggcgaaggc ccggatcgaa gccgccgggg gaaaggtctt
      421 gagcagcgtc agtgcaaaaa cgaacttcct ggtcgcgggc gaggcggcag ggtcgaagct
      481 tacgaaggcc caagagctgg gggtgaaagt cgttgatcag gcggggttgc tggcgttgat
      541 caagaagcga ggttgagttc ggaaatcgcc tagagtggcg gtgccatttt cgcccgcatc
      601 gacagccgta aggcgtgccg gtggagccgg gcaacccgag cggcgcgctc agtctccccc
      661 agctgctcga agcgctgagc ggccgataac agtgcgtcca acagatcacc gtcgaccgcg
      721 gttgcggact cttccaggag cgcttgagcg agggcctccg tttcagcgtc aatgacccgc
      781 tgggcaagct gcagggaaac cggaagagcc tcttggattg cggcggccaa caacctatcg
      841 atttgtcgaa gtcgctccaa gaccttcgaa tcggatcgtt cgcgggccac ccggatattc
      901 gcctgcaaga cgttcaggaa caaatcgtcg agcaacggca ggctggccgc gacggcggcc
      961 tccagatcct ggctgtttac taatgaagcc agcagtccgg aagcctgggc aaccctggcc
     1021 tcttgggccc ggtcgatctg ttcggtaatc tcgagcaagg aactgcgtag cttggtcagt
     1081 cgctcccccc gttcgggatc cacggtctcg atccgctcag agagcagctg aaagaagcca
     1141 taatcgagac cggggcgggc gagggtggcg agggcccgga cccggtctgg actgggtgct
     1201 tcaacgacca attccagaag tttctctcga gtcagcgagt cgccggccgc ccgcaaactt
     1261 tccgctgcgc tgcgaacttc tgcctcctgg gcagccaatt ccttgccaaa taggctcaag
     1321 ctcaacgcgg cctctaggcg ctgcgaggcg gcctgcctcg aacggtcgtc tggggcggtc
     1381 tggaggctga gcgaaccgag ttggaagaaa cgttcgtcca gctcggcatc atgctgctgc
     1441 acgaacgcgg ccagggtctc cggagtggtc cggagcaagt cctcaaagag gcgaagtttt
     1501 gcgcgttggg cctccagctc ttctctggtg atcccgtcgg cctgcagcac gcgctccacg
     1561 agcccctgag ccgtcaagac tgcttggggt tggagcaggt atcccttacg ctgttctgcg
     1621 ggcaacccat caattgcctg attgatcagc cggcccacca gccgttcttg ttcattcttg
     1681 gctatcccga gctcgaccgg aagataagtc aacagcagct ccttggccgg gtcgtggtaa
     1741 acgatcggag ttgcgagctg gccctcatat ccgcagaccg ggcagtgtaa gtggttcaga
     1801 gtcccagaaa gcaggcgggc cttcgcggct ggattctgtc cgacgtccac cagctgcaga
     1861 atctgggctt gaattggatt ccggcaattc gggcagcgga cggcagtgct cattcgcgct
     1921 ccctggggat agagaagcaa atccgggcgc cccgttcgac cttctcgtcc acccaaatcc
     1981 ttcccccatg agcttccacg actgccttcg ccaagaacaa ccccaggccg acgccctggg
     2041 tggtccgggc ggtttctgcg gaccggaaga agcgttcgaa tactcgggtg cggtctgcag
     2101 gcgaaatccc ggggcctgca tcctgcacgc acagtacgac ctccttaggt gccacccgac
     2161 cctggatttg gaccacgccc ccggccgagg cgtacttgat cgcattcgaa agcaagttgg
     2221 agacaacctg ggtcaggcgc tcctcgtcgg cccgcacgat cggaaagccc ttgggcagct
     2281 ggacctcgaa cgaatggagg ttcgactggg tgcgaaatcg ctcggcgagc tctcgagcga
     2341 tctcggccaa gtcaacgtcg ctcagcgaga gggcgggggt gccggattgc agtctggaag
     2401 cctcgagcaa atcatcgatc aaatccgcca accgatccga ttcttcctca atcacggcca
     2461 ggctatcgcg gaccaccgag ggttcccagg ccgcatcatc ccggcgcagg gtccccacgt
     2521 aacccttgat cagtgcgacc ggggtgcgga gctcgtgact gataattgag atgaaggtgc
     2581 tcttcagctc ctcggcctct cgaaacttcg tgatatcgcg cagattggcc acgacaccga
     2641 gcagccgacc atcgccggaa taagttgggg cgtaggtgat cccaaggctg gaggtgccat
     2701 tatcctgtcg gaggaggtct ccttcaacgt agagctcggc agtggatgag agcggccagc
     2761 cgccggctcc ggcatcttcg agggtcaacc ccggctcgcg gcttgcccag cgtatgatct
     2821 cggaatggtc cttgccgatc acgaagtcgg ccgagtatcc ggtcagacgg gcgcaggctc
     2881 gattgaagcg agtgaagcgg tagctcggat ccagaatgaa gattccgtcg gcggctgatt
     2941 ccagtacccc atccaggtgg cggcgctgat cggccacctg ggtgtagagc cgggcattgg
     3001 aaacggcgat cgcggcctgg gaggcgaagg ctcccagcag cgcccggtcc tcccgagaaa
     3061 acacgccccg gtacgagcgg aagatgaaaa tgaccccaac gacttcctcc cgggcgatga
     3121 gcggcaaccc gacgccggtg agcaagccca tcgatgcggc ctgggtgatc cgctgcagcc
     3181 ggcgattgac ctccggaagc gcgaaccggg ccgggtcgtc gtgttcggga atgtccgcta
     3241 gcagtggatc gaggaacttc aggaaatcgg gattgatccc gtaggtggtg gttacccgcc
     3301 agccttcttg ctctcgcagg gcaatcaacc cggcggaact gctaagcaat tccgccgaaa
     3361 ggcggactat ccgttccagg accttctcga gatccaactc ctcggtgagt acccgggaga
     3421 tctcgagcag atagtcgcgc tgacggacgc ggaagtccgg cagcatgcaa tcagcggccc
     3481 tccgcggctg aaagggaagc ctccagaatc cggaggccct cgtcgagcag cccttggtcg
     3541 atgctcaggg ccggcacgac ccggatcgcg ctttcaccgg cgccaagcag cagcaggccc
     3601 cggtcaaagg cttcttgcag cacgcgctcc cgcagctcgt gatccggccg cttgctgcca
     3661 tgatccagca ccagctcagc cccgaccata agacc
//
LOCUS       DATDPQ010000036         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_796905, whole genome shotgun sequence.
ACCESSION   DATDPQ010000036 DATDPQ010000000
VERSION     DATDPQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796905"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VI520_00825"
     CDS             complement(<1..989)
                     /locus_tag="VI520_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HLF36455.1"
                     /translation="MTPARGLVAAQKTTPKTVLDDLGRVMKLAEFESALPKGVTTGLK
                     INISWQTWYPACSSAPWQIEGVILALREAGYQDLVGVHNDTVVVDTAVGELNNKHRFV
                     TDKYKVPCIYLYEQNFEWFEYQPKRPLLVLDKVYPKGLFIPRALVGLNIIHLPTIKTH
                     VFTTITGAMKNAFGGLLHRDRHWTHAVIHDTLVDLLMIQQDIHPGIFAVMDGTFAGDG
                     PGPRAMRWHQKDLLLASADQVAIDAVSAKLQGFDPMQIRFIRRAHELGLGIGDPRQIR
                     VVGYDIEQEKPWGFVQEDTLASRGQKAIYHGPLKPLEKLLLQSPLVPWSYFAS"
     gene            1102..1542
                     /locus_tag="VI520_00830"
     CDS             1102..1542
                     /locus_tag="VI520_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36456.1"
                     /translation="MDPFEYHRLGLSAFNPSRSARRQQRVEIYLPMGLAVLLVVASGV
                     LLRRAGVGDPSVWADVGLAFLLGMVLVLGLLLLVVVAAAAVGVWYLVRLLPPPFMEAR
                     TVLAKARQAALQASDRTAQAVILPSAGLSALRAAFSHLASIIQR"
     gene            1549..1758
                     /locus_tag="VI520_00835"
     CDS             1549..1758
                     /locus_tag="VI520_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36457.1"
                     /translation="MDSGWKGKVLLIGGLIGAFTGLGAAYLIISKSQEHDEPPKIGAG
                     DGVRLGLLLLGLVRQIGELGAGQEE"
     gene            1787..>2277
                     /locus_tag="VI520_00840"
     CDS             1787..>2277
                     /locus_tag="VI520_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36458.1"
                     /translation="MRPTRAFWFWGLLLIAGGAVSCRPIAPDSPPPPPSIAATLTPAS
                     TPTATLSPTPAPLRLWVSAALPNLLKSSATAIQELGGRAVEWVEDPANAEVRIEPSPA
                     RPLARWIYAAVAPFPSLRDGLTSQELQELWTEGSGVFVGTPDAEALAGLLGEASAQIM
                     PAET"
BASE COUNT          399 a          696 c          737 g          445 t
ORIGIN      
        1 ttgctggcga aatacgacca ggggacgagc ggagattgaa ggagcagctt ttccagcggc
       61 ttcagcggtc cgtggtagat tgccttctga ccccggctgg ccaaggtgtc ctcctggacg
      121 aagccccacg gcttttcctg ctcgatgtca taccccacca cccggatttg gcgcggatcg
      181 ccgatgccca ggccgagttc atgggcccgg cggatgaacc gaatctgcat cggatcgaag
      241 ccctgcaatt tggcagacac cgcatcgatc gccacctggt cggccgaggc gagcagcagg
      301 tccttctggt gccagcgcat cgcccgcggt ccgggcccgt cgccggcgaa ggtgccgtcc
      361 atgaccgcaa agattcccgg atggatatcc tgttggatca tgagcaagtc caccaaggtg
      421 tcatggatga ccgcatgagt ccagtgccga tcgcggtgca gcaagccgcc gaaggcattc
      481 ttcatggcac cggtaatcgt cgtgaacacg tgggtcttga tcgtcggcaa atgaataatg
      541 ttgagtccaa ccagcgcccg ggggatgaag aggcctttcg ggtaaacctt atccaggacc
      601 aatagcggcc gcttcggctg atattcgaac cactcgaagt tttgctcata aaggtaaatg
      661 cacggcacct tgtacttgtc ggtgacgaag cgatgcttgt tgttgagctc gccgaccgcg
      721 gtatcgacaa cgacggtgtc gttgtgcacg ccgaccaaat cctggtagcc ggcttcccgg
      781 agcgccagga tgaccccctc aatctgccag ggggcggagg agcaggccgg gtaccaggtt
      841 tgccaggaga tgttaatctt gaggccggtg gttacgcctt tgggcagggc gctctcgaac
      901 tcagccagct tcatcacccg accgagatcg tcgaggaccg tcttgggagt agtcttctgg
      961 gcagccacca atccgcgggc gggggtcatg agcaggattc taaatcagtg tcgctctcga
     1021 gcaaaggaac cgtcggtcct cgcagcgaac tgagtaaccg ctcagtcggc cgattcgacc
     1081 tgcatgctac aatacgccac gatggacccc tttgaatacc atcgactggg tctgagcgcg
     1141 ttcaatcctt ccaggtctgc tcggcggcaa cagcgggttg aaatctactt gccgatggga
     1201 ctggcagttc tgctggtcgt cgcgagcgga gtgctgctgc gacgagcggg agttggggac
     1261 ccgagcgtct gggccgacgt cgggctcgcc ttcttgcttg gaatggttct tgtcctgggc
     1321 ctgctgctgc tggtggtcgt ggctgcagct gcggtcggag tctggtactt ggtccggctc
     1381 ctgccgcccc cattcatgga agcgcgcacg gtgctggcca aggctcggca agcggcgctg
     1441 caggccagcg accgaaccgc ccaagcggtt atcctcccga gtgccggctt gagcgccctg
     1501 cgggcggcct tcagccacct ggcctcgatt atccagaggt agacccacat ggactccggt
     1561 tggaagggca aagtactgct catcgggggc ctgattggcg cattcaccgg attgggcgcc
     1621 gcgtacctca tcatctccaa gtcccaggag cacgacgagc ccccaaagat cggagccgga
     1681 gacggcgtgc gcctggggct gctgctgctc gggctcgtgc gccagatcgg cgaactgggg
     1741 gctgggcagg aggagtgagc ggattcgttc cctggaaccc gccctggtga gacccaccag
     1801 ggcgttttgg ttttgggggc tgttgttgat cgccggtgga gctgtgtcgt gtcgtccgat
     1861 tgcgccggat agcccgcctc caccaccatc catcgcggca accctgactc cggcctcgac
     1921 gccgaccgcg accctctccc caacccccgc cccgcttcgc ttgtgggtat ccgccgcgct
     1981 gccgaacctg ctcaagtcct cggcgactgc gatccaggag ctcggtggtc gggccgtcga
     2041 gtgggtggag gatccggcaa atgcggaggt gcgaatcgaa cccagtccgg cgcgaccgct
     2101 tgctcgctgg atctacgcag ccgtggctcc gttccccagc ctcagagatg ggttgacttc
     2161 ccaggagttg caggaactgt ggaccgaggg gtcgggcgtg ttcgtcggaa cgccagacgc
     2221 cgaggcatta gcaggcctcc ttggggaggc ttcggctcag atcatgccgg ccgaaac
//
LOCUS       DATDPQ010000037         8364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2366972, whole genome shotgun sequence.
ACCESSION   DATDPQ010000037 DATDPQ010000000
VERSION     DATDPQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366972"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2243)
                     /gene="dnaE"
                     /locus_tag="VI520_00845"
     CDS             complement(<1..2243)
                     /gene="dnaE"
                     /locus_tag="VI520_00845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLF36459.1"
                     /translation="MSFVHLHVHSEYSLLDGLSSIPALVRRAKELGMPAVALTDHGSM
                     FGTMHFYQAAREVGIKPLLGIEAYLASRGMEDRDPELDGQAFHLLLLAESETGYRNLL
                     KIATAAQLQGFYYRPRIDREFLAAHSEGLICTTGCLSGEVPRALFQGREPHARKLLDW
                     YFEVFGRDRFFFELQQHAVPGLQQVNQQLIDLAPKYRARLVATNDVHYINPEEADLQD
                     VMLCIQTGSVRSDPDRMRMSDASYYLRSPAEMTELFGHVEGAIENTLWIAERAQVDLD
                     FEGYRLPPFAVPESHTGQSYLRERCLEGLERRYGSRAGEPQLRERLDYELRVIHEMGF
                     DAYFLIVWDLCRFAREQGIWYNARGSAAGSIVAYCLRITLVDPIEHGLIFERFLNPGR
                     VSMPDIDLDFQDDRRYLMLEYSAKKYGEDRVAQIITFGTLGARAAIRDVGRVMDIPLP
                     EVDRLAKLIPNIPGKPVTIPEAIETVPALREVIEGNGYLKELVETAAKLEGVARNAGT
                     HAAGVIITDRPITDYIPLHRPTKGSPEDSPVRAVTQFEMGVLESLGLLKIDYLGLSTL
                     TVMARACDLIRRRHAVELDIDSIPLDDPKTFELLGSGNVLGVFQVEGAGMRRHLVDMR
                     PTKLAQVTAMVALYRPGPMEFIPAYIRRMHGAEAVTYRHPALKPILEETYGITVYQEQ
                     IMYTAMQLAGYSAAEADNLRQAVAKKKAGALRQQRQKFVQGAVSNRIPEDTANAIFDD
                     WEAFARYG"
     gene            complement(2432..3481)
                     /locus_tag="VI520_00850"
     CDS             complement(2432..3481)
                     /locus_tag="VI520_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36460.1"
                     /translation="MKRSVGVLVLVLAGMLAFPSQAFAGGPEQGQVVFGGSFTLESGQ
                     ILEGDLVIFGGSADLEEGSLVEGNLFLMGGNAVIDGQVNGDLVLLGGNVDLGPKASIG
                     GDVVTLGGNLDRAPGSTIAGDVVAGDEFSFPFSFNFPSTTRVVPLNEFRFRFSPVLDV
                     LWFGLRSLLVAALATIVVMFWPEPTARAARAVVAQPLLSGGLGLLTFVVAPVLLIVLA
                     ITIILSPVSLLAAVLLVLGGVFGWIAIGKEVGDRLVKAFTWDLHPAASAGLGTLALSL
                     VVGGIGLIPCVGWMVGFVVVAIALGAVLLTRFGSREYAPAPAAAPTPAPVAIAPAAPR
                     KRAPKKRAAKKTSSK"
     gene            complement(3478..3966)
                     /locus_tag="VI520_00855"
     CDS             complement(3478..3966)
                     /locus_tag="VI520_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36461.1"
                     /translation="MSPPLDPGPLGHPASVPHPSDTEWDLIEAQLRRSPPLGPAVGFS
                     RRWLARLEQHREARRHRQVQLMLGGLLLGALLSLALVGLEALRLLGSPAGLAASWIRA
                     GLSARGTLEGSYLFLREIGGGLPAVFGGLAVALTIGWVSAVWFAALYRYSFANLPNGE
                     TK"
     gene            complement(3950..4534)
                     /locus_tag="VI520_00860"
     CDS             complement(3950..4534)
                     /locus_tag="VI520_00860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF36462.1"
                     /translation="MEPSEEGRWITAAQGGDDLAFARLVEHYAKPVHNLCYRMLGNSA
                     DAEDAAQETFLKAYQKLKSYDPLRPFPTWLLSIGAHHCIDQLRRRRFQLISLEALHRR
                     PAGRESQPERLAEHAREQALLQAELAKLGPYERAAIVMHYWYDLSYQEIGAALGLSVS
                     AVKSRLHRARRDLAARAETERQASGREPTGVTTA"
     gene            4685..5221
                     /locus_tag="VI520_00865"
     CDS             4685..5221
                     /locus_tag="VI520_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLF36463.1"
                     /translation="MAKLSSISQKFILHWGEMGTRWGVNRTVAQVHALLYVSPKPLNA
                     EEIATTLSIARSNVSYSLRELQNWGIIKPVHLLGDRREHYESLQDVWYMFQIVLDERK
                     RREVDPTVAVLRECVQELERKGDLADAHTRERLQEMLEFFEGATAVYDEVRRLSLDAI
                     RRLVRSGSRLQKILSSVA"
     gene            5367..6419
                     /locus_tag="VI520_00870"
     CDS             5367..6419
                     /locus_tag="VI520_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36464.1"
                     /translation="MRNAAYVILALGGYAFLAFGGISLELGNDYLFFHLYLTWLPFGA
                     FIWWRLSRFATRRLPRSLLFGAIFSPSLFGIYVTTPVIVSLLLIIYYLIADPWVLSVG
                     SLSLGDATLWLALTLLPFLMIWIGAFAVMSIWPARRKPTLPDPIDENIRSPMTAEKKK
                     DQALIAGLTVLVVVLIGFSLAMLSEREQATHDFESAEVLLTAIAAQEARTRGHYALSF
                     SSAGTGPVTIPAGGLQLFLFQPSELAQPIDFVKSLTLHLTSAETTRTGPTTLDLTLWD
                     FESDFGFKLMDTQLTWGENSIEIRRPDAYVSRAGWIYLEVRNFGEGEDVGLADLNFTL
                     IVLNDDGTETIYAPTQ"
     gene            6484..6900
                     /locus_tag="VI520_00875"
     CDS             6484..6900
                     /locus_tag="VI520_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36465.1"
                     /translation="MDIVWTYLIYLALSIGVTVGVARTLHKNGRVFLVEAFHGNELLA
                     DSVNHLLVVGFYLINVGYLTLRLKYGVKPTDLAESIEFLSSKVGLVLLVLGIMHFLNL
                     YIFSRMRRRAKRELELVQGARSTTRRTTRPKKLGHA"
     gene            complement(6966..>8364)
                     /locus_tag="VI520_00880"
     CDS             complement(6966..>8364)
                     /locus_tag="VI520_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36466.1"
                     /translation="LPLFILPARRIGETLRRIARQQMDLNARMNALMNETLNISGALL
                     VKIFGRYGLEVQRFRERASQVRDVGVRQAIIGRWFFLALGLVSAIGSALVFWLGGHLV
                     LGGSFTIGTLVAFTGYLANLYGPLMALTNARVDFATSMVSFERVFEVLDLPVEIQDKP
                     EARSIQTVEGGIRFEHVSLSYLSEGANPVRLEEVRRFGWGHSGTVTPTGKPMLRRDDQ
                     PAIPIGDGRPPGPDERRWALRDVSFEIAPGQVAALVGPSGAGKSTITYLLPRLYDPTE
                     GRILLDGDDLRDIALESLSEQVGMVTQETFLFHDTVRNNLLYARPDASQEAVERACQA
                     ANIHDFIAGLPEGYDTLVGERGYRLSGGEKQRVAIARVVLKDPRVLILDEATSHLDSQ
                     SEALIQEAMEQVMKGRTSLVIAHRLSTIIGADLILVLDEGRLVEQGNHQALLAGSGLY
                     RTLYETQFRPERLPA"
BASE COUNT         1673 a         2607 c         2436 g         1648 t
ORIGIN      
        1 ccgtagcgag cgaaggcctc ccaatcgtcg aagatcgcgt tcgcggtgtc ctccgggatc
       61 cggttgctca ccgccccctg cacaaacttc tgtcgttgtt ggcgcagggc cccggccttc
      121 ttcttggcca ccgcttggcg caggttgtcg gcttcggccg ctgagtatcc ggccagctgc
      181 atcgcggtgt acatgatttg ttcctggtag accgtgatcc cataggtctc ttccagaatg
      241 ggcttgaggg ctgggtggcg ataggtgacc gcttccgctc cgtgcattcg gcgaatgtag
      301 gcagggatga attccatcgg tcccggacgg tagagcgcca ccatggcggt tacctgagcc
      361 agcttggtcg gccgcatgtc cacgaggtgg cgacgcatgc ccgcgccttc cacctggaag
      421 acgcccagca catttccgct gccgagcagt tcgaaggtct tggggtcatc caggggaatg
      481 gagtcgatat ccagctcaac cgcatgccga cggcgaatga gatcgcaggc cctggccatc
      541 acggtcagcg tcgacagccc cagatagtca atcttcagca atcccaacga ttccaagacg
      601 cccatctcga attgggtaac cgctcggacc gggctgtcct ctggggatcc cttggtcggc
      661 cggtgcagcg ggatgtagtc tgtgatgggc ctatcggtga tgatcactcc cgcggcgtgg
      721 gtacccgcgt tgcgggccac tccttccaat ttggcggcgg tttctaccag ctctttgaga
      781 tagccgttcc cctcgatcac ctcccgcaaa gcaggaacgg tctcgatcgc ctctgggatc
      841 gtcaccggct tcccgggaat gttggggatc aacttggcca gccgatcgac ttctggcagc
      901 gggatatcca tcacccggcc gacgtctcgg attgctgccc gggcgccgag ggtgccgaag
      961 gtgatgatct gggcgactcg gtcttcacca tatttcttgg cgctgtactc gagcatcaga
     1021 tagcgccggt cgtcctgaaa atccaggtcg atatccggca tcgagacccg gcctggattc
     1081 agaaaccgct cgaagatcag cccgtgctct attggatcca ccagcgtgat ccggagacag
     1141 taggccacga tcgagcccgc ggcggagccg cgggcgttgt accagatgcc ctgttcacgg
     1201 gcgaagcgac aaaggtccca tacgatcaag aagtaggcat cgaagcccat ttcatggatc
     1261 acccgcagtt catagtccag gcgttcccgc agttgcggct cgcccgcccg ggagccgtag
     1321 cggcgctcga ggccctccag gcaccgctct cgtaagtagc tttgtccggt gtgtgattct
     1381 gggaccgcga agggcggcag gcgatagcct tcaaaatcca gatccacctg agcccgctcc
     1441 gcgatccaga gggtgttctc gatcgcacct tccacgtgcc cgaacaattc cgtcatctcg
     1501 gcgggtgacc gcaagtagta ggaagcatcg ctcattcgca ttcgatccgg gtcgctgcga
     1561 accgagccgg tttgaatgca gagcatgaca tcctggaggt cggcctcttc cgggttgata
     1621 tagtggacgt cgttggtcgc caccagacgg gcccggtatt tgggcgccag atcgatcagc
     1681 tgttgattaa cctgctgcag gcccggaacc gcatgctgct gcagctcaaa gaagaaacga
     1741 tctcttccaa acacctcaaa gtaccaatcg agcagtttcc gggcatgcgg ctcgcgccct
     1801 tgaaacaagg cgcggggcac ttccccggaa agacagccgg tcgtgcagat caatccttcg
     1861 ctgtgagcgg ctaggaattc ccgatcaatg cgcgggcgat agtagaagcc ttgaagctgg
     1921 gccgcggtgg cgatcttgag caggttgcga tacccggtct cgctctccgc cagcaagagc
     1981 aagtggaaag cctggccatc cagctcggga tcccggtctt ccataccccg gctggcaaga
     2041 taggcctcga tcccaagcag cggcttgatc ccaacttctc gggcggcttg atagaagtgc
     2101 atagtcccga acatggaccc atgatcagtg agggcaaccg ccggcatccc aagctctttg
     2161 gctcggcgca ccagcgccgg gatcgagctc aagccgtcta gcagtgaata ttcagaatgg
     2221 acgtggagat ggacgaagga catttccccc cagggaatgt gctcggcgat tatagcggag
     2281 ccgggctgta ggtgccagaa gtgggtggat tcaaggactc ttttaaggtt cgatcgaccg
     2341 agtctgactc agagtagaac ataacgagcg agaggcacgg tctcccgtgc ctctcgaatg
     2401 caaccgcttc tgcctgcggg aatcttgccg gctacttgct ggaggttttc ttcgccgctc
     2461 tcttcttcgg cgccctcttc ctcggcgcag ccggagctat ggccaccggt gcgggcgtgg
     2521 gggccgcggc gggcgccgga gcgtactccc gagagccgaa gcgggtcagc aacactgctc
     2581 ccagcgcgat ggccacaacc acgaagccga ccatccaccc tacgcagggg atcagaccaa
     2641 ttccaccgac cactaagctg agcgccaagg tacccagtcc ggcggaggcg gcaggatgca
     2701 aatcccaagt gaatgccttg accagccggt cgcccacctc cttgccgatg gcgatccaac
     2761 cgaacactcc tcccaggacc agcagcacgg cggccagcag gctgactgga ctcaagatga
     2821 tggtgatggc cagtacgatc aagagcactg gcgcgaccac gaaggtcagc agccccagcc
     2881 caccggacag cagcggctga gcgaccactg cccgggcggc gcgggccgtc ggctctggcc
     2941 agaacatcac tacgatcgta gctaatgccg caaccagcaa gctgcgcagc ccgaaccaga
     3001 gcacgtcgag caccggtgaa aaccgaaacc ggaactcgtt caagggcacg acccgggtgg
     3061 tagaagggaa gttgaaactg aatgggaagg agaattcatc gccggcaacg acgtccccgg
     3121 ctatcgtcga gccgggtgcc cggtccagat tccctcccag ggtcacgaca tcaccgccga
     3181 tcgaagcctt gggccccaga tcgacgtttc cccctagcaa caccaggtcg ccattcacct
     3241 gtccgtcgat taccgcgttg cctcccatca ggaagagatt gccttcgacc agtgacccct
     3301 cctccaggtc ggccgagccg ccgaagatca ccaaatcgcc ctcgaggatc tgcccgcttt
     3361 ccagcgtgaa gctgcctccg aaaacgacct gaccttgttc gggccctccg gcaaaggcct
     3421 gcgatgggaa cgccagcatg cccgccaaca caaggaccaa tacgccaacc gatcttttca
     3481 ttttgtttct ccattcggca agtttgcaaa agagtaacga tagagcgcag cgaaccatac
     3541 cgcgctcacc catccgatag tcagggcaac ggccaagccg ccaaaaactg ccggaagtcc
     3601 gccccctatc tctcttaaga acaggtagct gccttccagc gtcccccggg cactcagccc
     3661 cgcccggatc cagctagcgg ccaacccagc cggcgatccg aggagccgca gagcttcgag
     3721 ccctaccaat gccagtgaga gcagcgcccc aagcagcaag cctcccaaca tgagctgcac
     3781 ctgtcgatgt cgccgcgctt ccctatgctg ttccagtcgc gccaaccagc gccggctgaa
     3841 accgacggcc ggcccgagcg gtggggaacg gcgcagctgg gcttcgatca ggtcccactc
     3901 agtatccgac gggtgaggga ctgaagccgg gtggccgagc ggcccagggt caagcggtgg
     3961 tgacaccagt gggttccctt ccactcgcct gtcgttcagt ttctgcgcgg gccgccagat
     4021 cgcgccgcgc tcgatgcagc cgactcttga ccgcgctgac cgaaagtcca agcgccgcgc
     4081 cgatctcctg gtacgaaagg tcgtaccaat agtgcatgac gatcgcggcc cgctcatagg
     4141 gtccgagctt tgccaactcc gcctgcagca aagcttgctc ccgggcgtgc tcagccagcc
     4201 tctccggctg tgactcacga ccggccggcc ggcggtgcag cgcctccagt gagatcaatt
     4261 ggaaccgcct gcggcgcagc tgatcaatac agtgatgggc cccaatcgag agcagccaag
     4321 tgggaaaggg tctgagcgga tcatagcttt tcaacttttg gtaggccttc agaaacgttt
     4381 cctgggcagc gtcttccgca tcggctgaat tgcccaacat gcgatagcac aggttgtgga
     4441 ccggcttcgc atagtgctcg accaacctgg caaaggctaa gtcgtctccg ccctgtgccg
     4501 cggtgatcca tcgaccctcc tcgctcggct ccattgaaag ctgcgatctc atcaatatct
     4561 acggaagctt tggcaagaag ttgcggggca gatccgcttg gagggcttga gtgggtccat
     4621 ggcttgacaa gccgctgatt ctcctgtata ttatgtactg taacgactga agtaacagaa
     4681 aaaaatggca aagttatcgt cgattagtca gaagtttatc ctccactggg gagagatggg
     4741 cactcgctgg ggtgtcaatc ggaccgtggc gcaagtgcac gcactgctct atgtctcccc
     4801 caagcctttg aatgccgaag aaatcgcgac gaccctctct atcgcacgct ctaatgtcag
     4861 ctatagcctg cgagagcttc agaattgggg catcatcaaa ccggtccatc tgctgggaga
     4921 ccgccgcgag cattacgagt cgctacaaga cgtttggtat atgttccaga tcgtgctcga
     4981 cgagcgcaaa cgccgggaag ttgatcccac cgtggctgta ctccgtgagt gtgttcagga
     5041 attggaacgg aaaggggatc tagcggatgc ccacacccgt gagcggctgc aggagatgct
     5101 ggagtttttt gaaggtgcaa ccgcggtcta tgacgaagta cgtcggctct ctctagatgc
     5161 catccggcgc ctagtgagat ctggcagccg gcttcagaag atcttgagct ccgtcgcatg
     5221 aatgcggaat ctgggccttt tttctttgaa tgtttgtttc agtgcgtaca gaaatgattg
     5281 acatgaaacg cgcttcccca tcctcccgaa caccggcctc gatccggcat cggggaacaa
     5341 acgaggcctg aaaggggata tcccgcatgc gaaacgccgc atacgtcatt ctggcccttg
     5401 ggggatacgc cttccttgcg ttcgggggga tatcgcttga gttgggcaac gactatttgt
     5461 ttttccattt gtatttgacg tggttgccat tcggcgcatt catctggtgg cgactctctc
     5521 gtttcgcgac ccgtcgattg ccacgctcgc tgttgtttgg tgcaatcttc agcccgtcgc
     5581 tcttcggtat ctatgtaacc acaccagtga tagtttcctt gttgttaatc atctactact
     5641 taatagccga tccctgggtc ctgtcggtcg gcagtctgtc tctaggggat gccactttgt
     5701 ggctcgcact caccctgctg ccctttctca tgatctggat tggcgcgttc gcggtcatgt
     5761 caatatggcc ggctagacgg aaaccaaccc tgcctgaccc catcgatgaa aacatccgca
     5821 gtccgatgac cgccgagaaa aagaaggatc aagcgttgat cgctgggctt acggtgctgg
     5881 tcgttgtctt gatcggcttc tccctggcga tgctgagcga gcgtgagcaa gccactcatg
     5941 acttcgagag tgcggaggta ctgctcaccg caatagccgc ccaggaggca aggacccgcg
     6001 gccattatgc gttgtccttt tcctcggcag gaacgggtcc ggtcacgatc ccggccgggg
     6061 gcctgcaact gttcctcttt caaccgagcg agcttgcgca gccgatcgat ttcgtgaagt
     6121 cgctcacctt acatctcacc tccgccgaga caacccgaac cggtccaacc acgctcgatc
     6181 tcacgctctg ggactttgag agtgattttg gattcaaact gatggacacc caactaacct
     6241 ggggcgagaa ttccattgaa atccgcaggc ccgacgccta tgtcagccga gcaggctgga
     6301 tctatctgga agtccgaaac ttcggcgaag gagaggacgt tgggctggca gacctgaact
     6361 tcacgctcat cgttctgaac gacgatggga ccgaaaccat ttatgcgccc actcaatagt
     6421 cagctggccc gggctcacct cggacacctc ccgttgatat ctgggaactg aaaggaggcc
     6481 aatatggaca tcgtctggac ctatctcatc tacctggctc tgagtatagg ggtcacggtg
     6541 ggggtggccc gcaccctgca caagaacggg cgggtcttcc tggtagaggc gttccacggc
     6601 aacgagctcc tggcggattc tgtgaaccat ctgctcgtgg tgggcttcta cttaatcaac
     6661 gtaggctact tgaccctacg tctcaagtat ggagtcaagc ccaccgacct ggcggagtcg
     6721 atcgagtttc tcagctcaaa agtcgggctc gtcctcctgg tgctggggat catgcacttc
     6781 ctcaacctat acatcttctc gaggatgcgc aggcgcgcca agcgcgagct tgagctggtc
     6841 caaggcgccc ggtccacgac ccggcgcacg acaagaccga agaagcttgg ccacgcctga
     6901 acgaagggtg acccggatcg accaagcgct ctgccagggc gctgcctgct gaggtgggcc
     6961 ccagttcagg ccgggagacg ctccggcctg aattgggttt cgtacaatgt acggtagagc
     7021 ccgctgccgg caagcaaggc ctggtggttc ccttgttcga ccagccgccc ctcatcgagt
     7081 accaggatca gatcggcacc gatgattgtg ctcagccgat gggcgatgac cagggacgtt
     7141 cgtcccttca tgacctgttc catggcctcc tggatcaagg cctcggactg ggaatcgagg
     7201 tggctggtgg cctcgtccag gatcaacacc cggggatctt tgagcacaac ccgggcgatc
     7261 gccactcgct gtttttcgcc tccggagagc cggtagccgc gctcgccgac cagcgtgtca
     7321 tatccctccg gcagcccagc aataaagtcg tggatattgg cggcctggca ggcccgttcg
     7381 acggcctcct ggctggcgtc cggccgtgca tagagtaagt tgttgcggac cgtgtcgtgg
     7441 aacaagaagg tttcctgggt caccattccg acttgttcgg agagcgactc gagtgcaatg
     7501 tcccgtagat catccccatc cagcaggatc cgcccttcgg tcgggtcata caaccggggc
     7561 agcaagtagg tgattgtcga tttgccggct ccactcgggc cgaccagtgc ggccacctgg
     7621 cccggcgcga tctcaaagct cacatcgcgg agcgcccagc gtcgctcatc cggcccgggc
     7681 ggccggccat ccccgatcgg gatcgccggc tggtcgtcgc ggcgcagcat gggcttgccg
     7741 gtcggcgtga ccgttccgct gtgtccccat ccgaaacgac ggacctcctc cagcctgacc
     7801 ggattggccc cctccgacag gtagctcagc gagacatgtt cgaaccgaat acccccctcg
     7861 acggtctgga tggaacgcgc ctccggcttg tcctggatct ctaccggaag atcaagaact
     7921 tcgaataccc gttcgaagct caccattgag gtggcgaagt ccacccgagc gttggtgagg
     7981 gccatcagcg gtccgtacag gttggccagg tatccagtga aggcgaccag agtcccgatc
     8041 gtgaacgacc cacccagcac cagatgacct cccagccaga agaccagcgc gctgccgatg
     8101 gcgctgacca ggcccagcgc caggaagaac cagcggccaa tgatcgcctg ccggaccccg
     8161 acatctcgca cctgcgaggc ccgctcccgg aatcgctgca cctccaaccc gtagcgacca
     8221 aagatcttca ccaacaaagc gccgctaatg ttcagggttt cgttcatcaa ggcgttcatc
     8281 cgcgcattca gatccatttg ctggcgggcg atccggcgca gggtctctcc gatccggcgg
     8341 gccggcagga tgaacagggg cagg
//
LOCUS       DATDPQ010000038         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1843352, whole genome shotgun sequence.
ACCESSION   DATDPQ010000038 DATDPQ010000000
VERSION     DATDPQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843352"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1144
                     /locus_tag="VI520_00885"
     CDS             176..1144
                     /locus_tag="VI520_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36467.1"
                     /translation="MFAIGLRQLQQALDQKANQVQQEFILASEQLEEVGRKLLEPVPE
                     DRDRLREQQLSLRARQQALADEVNTWRERARGVRSQSGAHSLRTYLNELLKLDDPLLT
                     PAIQHSLYLLDAPEEELAKLAGQESPSVITTPAGRLIQRSRTEFDLRGSDAGARQRSA
                     IEFANRPGLAQQDAVIAEIEAAIEDPDPIVRETALLTMIQLHRFRSMRSADLDQAHAS
                     VQKLAALNHLTAIAPLIEILERPRTGFVQTESGSEEADNNRSRMVALLRLIEWHTPEA
                     KMAVHKLQFDRDPNLVKTARRALELFPDEWSGPLKATGPLPSFEEP"
     gene            1318..>3735
                     /locus_tag="VI520_00890"
     CDS             1318..>3735
                     /locus_tag="VI520_00890"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLF36468.1"
                     /translation="MADLTLAKSYEFKPVERRLYAEWERSGYFRPTGEGTPFVISMPP
                     PNITDRLHVGHALFVATEDLMIRHARMTGSPTLWLPGTDHASIATELQLRELLLREGT
                     SKHELGREAFLERAWQWKRKYGGIITEQLRRLGASCDWERERFTLDEGLSRAVTEAFV
                     RLHERGLIYRGDYLINWSPGLQTAVSDLEVEYTEEEATLYYFKYRVDGFEQPIPVATT
                     RPETILGDTAVAVHPEDERYRKFIGATARVPILDRPIPVIADQYVDREFGSGALKVTP
                     GHDPADYQIGLRHKLPIVNAMNPDATMSEAAGPYAGLDRYACREQLWADMQAAGLTIR
                     TEPYLQQVPRSQRGGEIIEPLISSQWFVRMQPLAEPALTAVRDGRIRIVPERFTKVYF
                     NWLENIRDWCISRQLWWGHRIPAWHCGDCREITVARAAPQRCPHCGSASLEQDPDVLD
                     TWFSSGLWPFSTLGWPEDTPDLRRFYPTTLMETGYDILFFWVARMIMMGLEFTDQVPF
                     TTIYLHGLIRDERGRKMSKTLGNVIDPLELMDEYGTDALRFALLTGSGPGNDLSLSLE
                     RVAAGRNFANKLWNAVRLILGAIAAAPEGPAGQPPRTPADIWILGRLNQVIREVDRLF
                     AGHQYGEAGRQIHDFFWGEFADWYLELSKIQTEQGGDRAWLTGRVLVHVFHNCLRLLH
                     PYMPFVTEELFGALRSACEAHPAHYGPKRGWEPALIVAKWAEAGPEEAWEQEGVAAFQ
                     IVQELVTAIRNARAEKGVEAGRRIEASIRAGERAEFLQANRAGLARLARLDPNRLQIE
                     AALEPPPN"
BASE COUNT          703 a         1168 c         1197 g          667 t
ORIGIN      
        1 ggtggcacat ccggggtagg ccatccgccc agttgaccct gggggggtcc ttcactatac
       61 tcacccattg ggcgtcctca ggcccagaac tgctagccct tcttggcgag cctctgattt
      121 cgttcccacc ggcctcttcg aatatgtagg cagcgaccaa ggagggaagc aaagcgtgtt
      181 cgcaattggc ctgcgccaac tgcagcaggc gctggatcag aaggccaatc aggtccagca
      241 agaattcatc ctggccagcg agcagctgga agaagtcggc cgcaaactgc tcgagcccgt
      301 cccggaggac cgcgaccgcc ttcgagagca gcagctctcg ctgcgggccc gtcaacaggc
      361 cctggcggac gaggtcaaca cttggaggga gcgggcccgc ggagtgcgca gccagtccgg
      421 cgcccactcc ctccgcacat acctcaatga attgctcaaa ctcgacgacc cgctgctgac
      481 cccggcgatc cagcactccc tctacttgtt ggatgccccc gaggaggagc tggccaagct
      541 tgccgggcaa gagtccccaa gtgtcattac cacgcccgcc ggccggctca tccagcgttc
      601 tcgaacggag ttcgatctgc gcggatcaga cgccggggcg cggcagcggt ccgcgatcga
      661 gttcgccaat cgcccgggcc tcgcccaaca ggacgcggta attgcggaga tcgaggccgc
      721 catcgaagac cccgatccga tcgtccgaga aactgccctc ctgaccatga tccagctgca
      781 ccggttccgc agcatgcgat cggcggatct cgatcaggcc cacgcttcgg tgcagaaact
      841 ggccgctctc aaccatctca ccgccattgc tccgctgatt gaaatcctgg agcgtccgcg
      901 caccgggttt gtgcaaaccg aatctggctc ggaagaggcc gacaacaatc gatcgcggat
      961 ggtggccctg ctgcgcctga ttgagtggca cacccccgag gcgaaaatgg ccgtacacaa
     1021 gctacagttt gaccgcgacc cgaatctggt taagactgcc cggcgggcgc tggagctttt
     1081 cccggatgag tggagcggac cgctcaaagc caccggcccg ctgccgagtt tcgaggaacc
     1141 ctagtcgcgg ctgctcggtt gactcgtggg cgcgagcgcg gctataatcc cgcccaatgg
     1201 aataaagtgc tccgggagga gtacgcccgg ccctctcgtc ctcacgggcg agggggtttt
     1261 ttgttggaag gtcggaaaga tccggttggc gggcactccg agtcaggagg ttgaagcatg
     1321 gcggatttga cgctggcgaa gagctacgag ttcaagccag tcgagcgccg cctatatgca
     1381 gagtgggaac ggtcgggcta ttttcggccg acgggggagg gaactccctt cgtcatctcg
     1441 atgcctcccc ccaacatcac tgaccggctt catgtcggtc acgcattgtt cgttgccacc
     1501 gaggacttga tgattcgtca cgcacggatg actggcagcc ctacgctatg gctgcccgga
     1561 acagaccacg ccagcattgc gaccgaactg cagctgcgcg agctgctgct tagggaaggc
     1621 acttccaaac atgagttggg ccgggaggcc tttctcgagc gggcctggca gtggaaacgg
     1681 aagtatggag gcatcatcac cgagcagctt cgccggctcg gggcttcctg cgactgggaa
     1741 cgcgagcggt tcaccctgga tgagggcctc tcgcgggccg tcacggaggc cttcgttcgg
     1801 ctgcacgagc ggggattgat ctatcgcggc gattatctga tcaattggtc acctggcctg
     1861 cagactgcgg tgagcgatct cgaagtggag tacaccgaag aagaggccac tctgtactac
     1921 ttcaaatacc gggtcgatgg attcgagcag ccgatcccgg tggccacgac ccggccggaa
     1981 acgatcctgg gggacaccgc ggtggcagtc catcccgagg acgagcgcta tcgcaaattc
     2041 atcggtgcga ccgctcgggt cccgatcttg gatcggccga ttccggtgat cgccgatcaa
     2101 tatgtggatc gagagttcgg cagcggtgcg ctcaaggtga ctccgggcca cgatccggcc
     2161 gactatcaga tcggcctgcg gcataagctg ccgatcgtga acgcgatgaa cccagacgca
     2221 acgatgagcg aagcggcggg cccgtatgcc ggactcgatc gctatgcttg ccgcgagcaa
     2281 ctgtgggccg acatgcaggc ggccgggctg accatccgaa ctgagccgta tctgcagcag
     2341 gtgccccgca gccagcgcgg cggcgagatc atcgagccgc tgatctccag ccagtggttc
     2401 gtgcgaatgc agccactcgc cgagccggcg ctcaccgccg tgcgggatgg acggattcga
     2461 atcgttccgg agcgctttac taaggtgtac ttcaactggc tggagaatat tcgcgattgg
     2521 tgcatctcgc gccaactctg gtgggggcat cgcatcccgg cctggcactg cggcgattgc
     2581 cgcgagatca cggtagcccg ggcggcgccc cagcgctgcc cgcactgcgg ctcggccagc
     2641 ctggaacaag atccggacgt gttagacacc tggttctcgt ccggcttatg gccgttttct
     2701 accctcggct ggccggagga cacgcctgac ctgaggcgct tctaccccac gaccctgatg
     2761 gagaccggtt acgacatcct gttcttctgg gtggcccgga tgatcatgat gggtctggag
     2821 ttcaccgatc aggtcccgtt taccacaatc tatctgcatg gtctcattcg ggacgagcgc
     2881 ggcaggaaga tgagcaagac cctggggaac gtgatcgacc cgctggagct catggacgag
     2941 tacggcaccg acgcgctgcg cttcgcgctg ctcaccggct ctggccccgg caacgacttg
     3001 agtctgagcc tggaacgagt ggccgccggc cgaaattttg ccaacaaact atggaacgcc
     3061 gtccggctga tcctgggggc catcgccgcc gcgcccgaag gacctgcagg ccaaccgccc
     3121 cgcaccccgg ccgacatctg gatcctgggc cgcctgaacc aggtgattcg cgaggtggac
     3181 cgcctcttcg ccggacatca atatggggag gccgggcggc aaattcacga tttcttctgg
     3241 ggagagtttg ctgattggta tctggaactc tcaaagattc aaaccgagca aggcggcgat
     3301 cgggcctggc tgaccggccg agtgctggtt cacgtctttc acaactgcct gcggttgctg
     3361 cacccctaca tgccgttcgt gaccgaggaa ctcttcggtg ccctgcggtc ggcctgcgag
     3421 gctcatccgg cgcactacgg acccaagcgc ggttgggagc cggccttgat cgttgcaaag
     3481 tgggcggagg ccggaccgga ggaagcttgg gagcaggagg gggtggctgc tttccagatc
     3541 gtgcaagagt tggtcacggc aattcgcaat gctcgggccg aaaaaggggt ggaggccgga
     3601 cggaggatcg aggcctccat cagggctggc gagcgggccg agttcctgca agcgaaccga
     3661 gccgggctgg cccggttggc tcgcctggac cccaaccggc tgcaaatcga agccgcgctg
     3721 gagccgcctc cgaat
//
LOCUS       DATDPQ010000039         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_535943, whole genome shotgun sequence.
ACCESSION   DATDPQ010000039 DATDPQ010000000
VERSION     DATDPQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535943"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VI520_00895"
     CDS             complement(<1..630)
                     /locus_tag="VI520_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF36469.1"
                     /translation="MDPAVPPAVEPPGLLARLNWNDEFGERTIVFDTPSGTVKLRQVG
                     AGSLMLKFGETVVHVNPWSKAGEYESLPKADQIWITDPSPEHLDLHAIRAVSTDSTQL
                     IVDALSARQLRGLLSFVRLLDGVEIAVDEIELMALPAFRAGMSPDGPQLDPSNSYLAT
                     FGDFRLFLAGPAHFVPGIQGIEKVDVALLSIDDLSSLSSEQAAEITRALD"
     gene            942..1280
                     /locus_tag="VI520_00900"
     CDS             942..1280
                     /locus_tag="VI520_00900"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HLF36470.1"
                     /translation="MGQIQWPDFEKVDIRVGRVLEVDDFPEARNPSYLLRIDFGPELG
                     VKRSAAAIRDLYAKEEISNRLVVAVVNFPPKQIATHISEVLVLAGVNPDGSMRLLQPD
                     AEVQLGARVR"
     gene            complement(1284..1949)
                     /locus_tag="VI520_00905"
     CDS             complement(1284..1949)
                     /locus_tag="VI520_00905"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HLF36471.1"
                     /translation="MSWRVILGVGLVVIGGGFLLDQMGVVDFGPLLATWWPLILILIG
                     VIQLATRSVPALAGMFVVLLGVLFQVDRLDIININVGQLIWPLILISAGVFMLLNRSR
                     RSATVHSDDRLDSFVMFGGMDRRVNSQAFEGGSATAFFGGTEIDLRDAKLAPGGAQLD
                     LAAAFGGIELMVPENWKVHVSGMPIFGGWSNKARLREDAPADLPMLHVRCFVAFGGIE
                     VHN"
     gene            2032..3039
                     /locus_tag="VI520_00910"
     CDS             2032..3039
                     /locus_tag="VI520_00910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLF36472.1"
                     /translation="MKFDAQIEPASLSDVPLIAAGAERIGFDALWSSETQHDPFLPLA
                     LVAEHTRRLQFGTAVAIGFARSPATLAYTAWDLAEASGGRFILGLGTQVKPHIERRHG
                     MPWPDSPVGKMSELIAAVRAFWNAWQTGERLNFRGEYYKLTLMPPFFNPGPIEHPRIP
                     IYIAGVNTGFCRLAGELADGFHSHPYHSPRYLREVVRPAIAEGATRAGRPVEAISLST
                     TAFAVTDPQEAQLVRGQIAFYASTPSYRGVMELHGWGDTAEQLQGMARRGEWPAMGGL
                     ITEEMLEAFAVVCEPEGLGSALLERYQGLVDRLALYLPYVPGQRDPMWSSLVRSLRAI
                     V"
     gene            3137..3619
                     /locus_tag="VI520_00915"
     CDS             3137..3619
                     /locus_tag="VI520_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36473.1"
                     /translation="MPSAPLTKKQIRWARRWMTLVLVGLFITIIGVEPDLLSLDRSPV
                     VGFVQIGVWSTGLAILLLGATLTVRVVRNSRPNSLRSEVGVRLIATGYVFAVAASFAD
                     FLGIGSHTLPTIYFGPVQVIGLALSVLTSLLGLILYWPSRARRVEHAPSPAAASASAD
                     "
     gene            3636..4355
                     /locus_tag="VI520_00920"
     CDS             3636..4355
                     /locus_tag="VI520_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36474.1"
                     /translation="MDFSVETIRLNKAFGAIRAVAELDLQVQAGEIYGLLGPNGSGKT
                     TLMRLLVGLAKADKGEVRVLGRLMPDRALLAEVGYMTQAEALYSDLTARENVLFFAAL
                     SGVRTASWIGEALELVELEARANDLVGSLSGGLRRRVSLACALVHRPRLLILDEPTVG
                     VDPQLRVAFWEHFRRLAEAGVTLIISSHVMDEAGRCDRLGFMREGWLIAEGSPDELRQ
                     RSGMTDLEQAFLKFAEADHAA"
BASE COUNT          795 a         1354 c         1427 g          785 t
ORIGIN      
        1 gtccagcgca cgggtgattt cggccgcctg ctcagacgag agcgagctga gatcgtcgat
       61 actgagcagg gccacatcga ctttctcgat cccctgaatg cccggaacga agtgggccgg
      121 cccggccaga aagaggcgga aatcaccaaa ggtggccaga tagctgttgc tcggatccaa
      181 ctgcggccca tccggcgaca ttccggcccg gaaggcgggc agcgccatga gttcaatttc
      241 gtcaactgcg atctccaccc catccaatag tcgaacaaag gagagcagcc ctcgcagttg
      301 gcgggccgac agcgcgtcca cgatcagctg ggtggaatcg gtggagacgg cccggatcgc
      361 gtgtaggtcg agatgctcgg gcgacggatc ggtgatccag atctggtcgg ccttgggcag
      421 ggactcgtac tcgccggcct tcgaccacgg attcacgtga actacggtct ctccgaattt
      481 cagcatcagc gaaccggctc cgacctggcg cagcttgacc gttccgctgg gtgtgtcgaa
      541 tacgatagtt cgctcgccga attcatcgtt ccagttgagg cgggccagca ggccgggagg
      601 ctcgaccgcg ggcggaactg ccgggtccaa gggtccgctg agtgcagcga ccgacggggg
      661 agccgcaggc tccgggagcg aaaggataat tgaggccggc ggcgctagat gttcagaagg
      721 caatatggaa cagccgccca gcgcggctgc gctgagaagg gccacaagtg cgggaaggaa
      781 gaggcgtggg tggagggccc tatgcatcag gtcggggact ctagatgatt acaacacggc
      841 ttggggcgcg ctgtgaatag ctcgaccggg ccaagcccca ccggccaaag cgcattatgg
      901 ctggcgggct gtacaatcag cccacgacaa taggaaccgg aatggggcag atccagtggc
      961 cggacttcga gaaggtggat atacgggtgg gtcgggtgtt agaagtcgac gacttcccgg
     1021 aggcccgcaa cccgtcttac ttattgcgaa tagacttcgg tccggagctc ggcgtcaagc
     1081 gctcggcggc cgcgatccgc gacctctatg ccaaagagga aatcagcaac cgcttggtgg
     1141 tggcggtggt gaactttcca cccaagcaga ttgccaccca catctcggaa gtgctggtgt
     1201 tggctggagt caatccagac gggtcgatgc ggctgctgca gccggatgct gaagttcagc
     1261 tcggagcccg cgtccgttaa cggctagttg tggacctcga ttccaccgaa cgcgacgaag
     1321 cagcgtacgt ggagcatcgg gaggtccgcc ggcgcatctt cccgcagccg agctttgttg
     1381 ctccagccac caaagatggg catcccgctc acgtgcactt tccagttctc cggcaccatg
     1441 agttcaatcc cgccaaacgc agcggccaga tccagttggg cgccgcccgg ggcaagcttg
     1501 gcgtcccgca ggtcgatttc cgttcccccg aagaatgccg tggcactgcc tccctcaaag
     1561 gcctgcgagt tcacccggcg gtccatgccg ccgaacatca cgaagctgtc caaccgatca
     1621 tcggagtgga ccgttgccga gcggcggctc cgattcagca acatgaacac ccccgcggag
     1681 atcaggatca gcggccaaat gagctggccg acgttgatgt tgatgatatc cagccggtcg
     1741 acttggaaga ggactccaag cagaacgacg aacatgcccg ctaaggcggg caccgaccgg
     1801 gtggcaagct ggatgactcc aatcagaatg agaatcagcg gccaccaggt ggccaggagc
     1861 ggcccaaagt cgaccacccc catctggtcc agcaggaagc cgcccccaat caccaccaga
     1921 cccactccca gaattacgcg ccaggacatt ccctcctcct tccaactctc gccgattcat
     1981 tctacctgat gacctccagc caccggtacc gacgatgagc acggaaatcc cgtgaagttt
     2041 gacgctcaaa ttgaacccgc ctcgttgagc gatgtcccgc tgatcgcggc cggcgccgaa
     2101 cggatcgggt tcgacgctct ttggtcgtcc gagactcagc atgatccgtt tctaccgctg
     2161 gcgctggttg ccgagcacac tcggcgactg cagttcggca ccgcggtggc catcggattt
     2221 gcccgcagcc ctgccaccct ggcctacacc gcctgggatc tggccgaggc ttccggtgga
     2281 cgcttcatcc tggggctggg gactcaggtc aagccgcaca ttgagcggcg ccacggaatg
     2341 ccctggccag actcaccggt cgggaagatg agtgagctga tcgccgccgt gcgcgccttc
     2401 tggaacgcct ggcaaaccgg cgaacggctc aacttccggg gcgagtatta caagctcacc
     2461 ctgatgccgc cgttcttcaa tccgggaccg atcgaacatc ctcggatccc gatctacatc
     2521 gcgggagtca acactgggtt ctgccggctg gcgggcgagc tggccgacgg gttccattcg
     2581 cacccttatc attcgccgcg ctatctgcgg gaggtcgtgc ggccggccat cgccgaaggg
     2641 gcgacccggg ccgggcggcc ggtcgaggcg atcagcctat ccaccactgc ctttgcggtc
     2701 accgaccctc aggaagccca gctcgtacgc ggccagattg ccttctatgc ctccactccc
     2761 agctaccggg gggtcatgga gctgcacggc tggggggaca ccgccgagca actgcagggg
     2821 atggcgcggc ggggagagtg gcctgcgatg ggcgggctga tcacagaaga gatgctggaa
     2881 gcctttgcgg tggtctgcga gccggagggg cttggctccg ccttgctgga gcgctaccag
     2941 ggactggtag accggttggc gctctacctc ccctacgtgc ctggccagcg cgacccaatg
     3001 tggagcagcc tggtccggag cctccgggcc attgtatgag ccacccctgg gggtgctctt
     3061 tttgccctgg gaagcgcagg gaaagcgccc aacaggccgt cttgacagcc ccgccgaagt
     3121 ggggtttatt ggccaaatgc catctgcacc gcttactaag aagcagattc gctgggcccg
     3181 gcgctggatg accctcgtgc tagtcggcct tttcatcacc attatcgggg ttgaaccgga
     3241 cctgctaagc cttgaccgca gcccggttgt cggattcgtg caaatcgggg tgtggtcgac
     3301 cggcctggcg atcctgctgc tcggagccac cctgacagtg cgggttgtgc gcaacagccg
     3361 tcccaactcg ctccggtcgg aggttggggt gcggctgatc gcgacggggt atgtctttgc
     3421 cgtggcggcc tcgtttgcgg actttctggg catcggctcg catacgctgc ccacgatcta
     3481 cttcggcccg gtacaggtga taggactggc gctgagtgtg ctcaccagtt tgttgggcct
     3541 gatcctgtat tggccaagtc gggcaaggcg ggtcgaacac gcccccagcc ctgcggcggc
     3601 ctccgcctcc gccgactaga ccactcgaac cttcgttgga cttctctgtt gaaaccatcc
     3661 ggctgaataa ggccttcgga gctattcggg ccgtcgccga gcttgacctg caggtccagg
     3721 ccggcgagat ttatgggctg ctcggaccga acggttcggg aaagacgacc ttaatgcggc
     3781 tgctggtcgg cctggctaag gccgacaagg gcgaagtccg cgtgttaggg cggctaatgc
     3841 cggaccgggc cttgttggcg gaagtgggat acatgaccca ggcggaggcc ctctactctg
     3901 acctgaccgc ccgagaaaac gtgctcttct tcgccgcctt gagcggagtc cggaccgctt
     3961 cctggatcgg agaggccctc gaattggttg agctggaggc ccgggccaac gacctggtgg
     4021 ggtcgcttag cggcgggctg cggcggcggg tctcgctcgc ctgtgcactg gtccatcggc
     4081 cccggctgct gatcttggac gagccgaccg tcggggtgga cccacaactg cgggtggcct
     4141 tctgggagca ctttcggcgg ctggcggagg ccggggttac gctgatcatc tccagccacg
     4201 tgatggacga ggccgggcgc tgtgaccggc tgggcttcat gcgcgagggc tggctgatcg
     4261 ccgagggcag cccggacgag ctgaggcagc gcagcgggat gaccgacctg gagcaggcct
     4321 ttctcaagtt tgccgaggcg gatcatgcag cctagccggg t
//
LOCUS       DATDPQ010000040         3028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_14725, whole genome shotgun sequence.
ACCESSION   DATDPQ010000040 DATDPQ010000000
VERSION     DATDPQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14725"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VI520_00925"
     CDS             complement(<1..330)
                     /locus_tag="VI520_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36475.1"
                     /translation="MRNSWVIAVREFRRYFVSPLAYAAAALTFLVLGLIFSLNVNFGL
                     PTGQMSPDGRMLLGPLATVLLIVSPVLTMRLLAEEQRMGTLELLLTSPVRDWELVVGK
                     WLGSFLFL"
     gene            complement(334..1275)
                     /locus_tag="VI520_00930"
     CDS             complement(334..1275)
                     /locus_tag="VI520_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF36476.1"
                     /translation="MIEANQLSKTYGNFVALAGISFEAKPGEILGFLGPNGAGKTTTM
                     RILTGYMPPTSGSAKVAGYDVVEDSLEVRRRVGYLPETVPLYPEMSGREYLSFMGSLR
                     RVADLDSRVGEVLIQVGLQERGDSLIGNLSKGLRQRVGLAQALLHQPEVLILDEPTIG
                     LDPAQIIEVRDLIRELGRERTVMLSTHILSEAQQVCDRVLIISKGRIVAEDTPANLSA
                     QLAGADRILVRSSAPAGDLATLLKDVPGVESVEIARQAGTVEVTSTPGRDLRPLLARR
                     IVEADLDLLELTTTGFSLESIFLQLTREEDQAIAQES"
     gene            1556..>3028
                     /locus_tag="VI520_00935"
     CDS             1556..>3028
                     /locus_tag="VI520_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLF36477.1"
                     /translation="MQALTAFEQSAVLSVVGIAILGLVYALFLRTQVLREPVGTPRMQ
                     EVWNAIRVGADTYLRRQLSSILPLIAVLTVALFLSVYIVPPSPEALERFHSLNSDTVV
                     LVIGMGRSLAFVMGAIFSLTVGQIGMRMAVQANVRVAAAARRSFGDSLRIAYRAGTIT
                     GMLTDGLGLFGGTMIFIIFGIAAPDALLGFGFGGTLVALFMRVGGGIYTKAADVGADL
                     VGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVAGLILGLALWHI
                     TGNFEWVIYPLLVRGIGVLSSIIGTYAVRGGPGKSGDAMRAIFSGFLSSAAISVVLFG
                     IIGFFYMQDVPGGWWRPFLATTVGVLLAIAIDRLTEYFTGSHGRPVQEIRKATNTGPA
                     TTILSGIAVGYESSVWSVLVIGLTIFVSILTFGGISGIPAGEAVTFVLYGVAMTGIGM
                     LTLTGNNVAMDSFGPIADNANGIGQMAWHGEDDRDTVQARQ"
BASE COUNT          552 a          932 c          944 g          600 t
ORIGIN      
        1 gaggaagagg aatgagccca gccacttgcc aaccaccagc tcccaatcgc gcaccggtga
       61 ggtgagcagc agctccaggg tccccatccg ctgttcctcg gccagcaagc gcatggtcag
      121 caccgggctg acaatcagca agacggttgc cagcggcccc agcagcattc ggccatctgg
      181 actcatctga ccggtcggca gcccaaagtt gacgttcaag gaaaagatca ggccgagcac
      241 caggaaggtg agtgcggccg cggcgtaggc cagtggcgat acgaagtagc ggcggaactc
      301 acggaccgcg atcacccaac tgttgcgcac gaatcagctc tcctgcgcta tggcttgatc
      361 ttcctcgcga gtaagctgca ggaagatgct ctccaggctg aagccggtgg tcgtcagctc
      421 cagcaggtcc aaatcggctt cgacaatacg acgcgccaac aacggccgca gatcccggcc
      481 gggggtcgag gtgacttcca ccgtacctgc ctgcctggcg atctcgacgg actcaactcc
      541 cggaacgtcc ttcagcaagg tggccaggtc gcccgccggg gcagagctgc gtaccaggat
      601 ccggtccgct ccggccagct gggcagagag attggcggga gtatcctccg ccacgatccg
      661 ccctttgctg atgatcagca cccggtcgca aacttgctgg gcttccgaga gaatgtgggt
      721 ggacagcata acggtccgtt cccgccccag ctctcgaatt aggtcccgta cctcgataat
      781 ttgggccgga tccagaccga tggtgggttc gtccaggatc aagacctccg gctggtgcaa
      841 gagcgcctgg gccaggccca cccgctgtcg cagccccttc gagaggttgc cgatcaaact
      901 gtcgccgcgc tcctgcaggc caacttggat gagcacctcc ccgacccggc tgtccagatc
      961 cgcaactcgc cggagcgagc ccatgaacga gagatactcc cggccggaca tctcggggta
     1021 cagcggcacg gtctccggca ggtagccgac ccggcgccgc acctccaacg aatcttcgac
     1081 cacgtcgtat ccggcgacct tggcggaccc gctagttggc ggcatgtatc cggtaaggat
     1141 ccgcatcgtg gtcgtcttgc ccgccccgtt cggcccgagg aatcccaaga tctccccggg
     1201 tttggcctcg aaggagatcc ccgccagggc gacaaaattc ccgtaggtct tggaaagctg
     1261 attggcctcg atcaagcctc ggtccttaag ggcgggtggt gccggttctt ggaggttggc
     1321 tccacacaaa aacaacccgg ctctgccgtt gcaacgatct tgctccggaa gaccgggatg
     1381 tgaggggtcc gatcccgctg accactatac agaacttagc agccgagagt caagacacag
     1441 ttccgcctct cacgagatgc taagctcttg gcccggtttt ctttaagctt gcttgacatg
     1501 gcctgtgcca acttgtacaa tcgcccagct tcccccatag gagcactgga acctgatgca
     1561 agctctgacc gcatttgaac aaagcgcggt tctcagcgtt gtcgggattg ccatattggg
     1621 gttggtctac gcacttttcc tgcgaactca agtgttgcgc gagccggtcg gcactccccg
     1681 gatgcaggag gtttggaatg cgatccgggt cggagccgat acctacctgc gacgccagtt
     1741 gagcagcatt ctgccgctga ttgcggtgct cacggtcgcc ctcttcctca gcgtgtatat
     1801 cgtcccccct agcccagagg ccttggagcg attccactcc ttgaattctg ataccgtcgt
     1861 cctggtcatc gggatgggaa ggtcgctggc cttcgtaatg ggcgccatct tcagcttgac
     1921 ggtggggcaa attggaatgc ggatggcggt gcaggccaac gttcgggtgg cggcggctgc
     1981 ccgccggtcg ttcggggact cgctgcggat tgcctatcga gccggcacga tcaccgggat
     2041 gctgaccgac ggcctcggcc tcttcggcgg cacaatgatc tttatcatct tcggaatcgc
     2101 cgccccggat gcgctgctcg ggttcggatt cggcgggacc ctggtggcgc tcttcatgcg
     2161 ggtcggcgga gggatctata ccaaggcggc cgacgtcgga gccgatttgg tgggaaaggt
     2221 ggaggcgggg attcccgagg acgacccccg caatcccgca gttatcgccg acttggtggg
     2281 cgataacgtg ggcgactgcg ccgggatggc ggccgacata ttcgagtcat atgaagtgac
     2341 cattgttgcc gggctgattc tcgggcttgc gctgtggcac atcaccggga acttcgaatg
     2401 ggtcatctac cctctgctgg tgcgcggcat cggtgtgctc tcttcgatca ttgggaccta
     2461 tgcggttcgc ggcggcccgg ggaagagtgg cgatgcgatg cgggcaatct tcagcgggtt
     2521 cttgtcctcg gcggcgatct cggtggtcct attcggaatt atcggattct tttacatgca
     2581 agatgtgccg ggaggctggt ggcggccttt cctggcgaca accgttgggg tgctgctggc
     2641 gatcgccatc gaccggttga ccgagtactt caccggaagc cacggcaggc cggtgcagga
     2701 aatccggaag gccaccaaca ctggcccggc gactaccatt ctttccggga tcgcggtcgg
     2761 gtacgagtcg agtgtctggt cggtcctggt cattggactg accatcttcg tctcgatcct
     2821 caccttcggc ggcatctccg gaatcccagc cggcgaggca gttaccttcg ttctatatgg
     2881 cgtcgccatg accggtatcg ggatgctgac cctgaccggc aacaatgtgg ccatggattc
     2941 cttcggtccg atcgccgaca atgccaatgg aatcggccaa atggcctggc acggggagga
     3001 cgaccgcgat acggtgcagg cccgccag
//
LOCUS       DATDPQ010000041         3777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1323676, whole genome shotgun sequence.
ACCESSION   DATDPQ010000041 DATDPQ010000000
VERSION     DATDPQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323676"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VI520_00940"
     CDS             <1..598
                     /locus_tag="VI520_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36478.1"
                     /translation="ANGLVTGDGLAGLRSLLQPADLRRRAARARGRRPGFGPLGRWAV
                     LRRESTGTESDLAGLAARQLLRRYGVVIRELLARETRIPAWRALLRVYREMEARGEIR
                     GGRFVDGFVGEQFALPEAVDELRRVRRDTQPEPAVMVPASDPLNLVGILTPGSRISPH
                     SNQVILYERGVPVDVGELGAVRHRLRRSSLAEFEAREV"
     gene            complement(606..3200)
                     /gene="clpB"
                     /locus_tag="VI520_00945"
     CDS             complement(606..3200)
                     /gene="clpB"
                     /locus_tag="VI520_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HLF36479.1"
                     /translation="MELERYTQKAQSGLLAAQRLAEDRGHSSLEPEHLLLALLRQAEG
                     IVPALLTRIIGGTELLEAELESALSARPTVSGATSQIGLARSTNSVLKDAELRASGMK
                     DEYVSAEHLLLALAAGPLQKLLEKFGVTPDAILQALTSVRGSARVVSPTPEDSYQALE
                     KYGRDLTRLARQGKLDPVIGRDEEIRRLIQVLSRRTKNNPALIGDPGVGKTAIVEGLA
                     ARIVRGDVPEGLKRKRLVQLDLGSLVAGTKYRGEFEERLKAVLREVTEAEGEILLFVD
                     EMHTVVGAGAAEGAMDAGNMLKPLLARGELHLIGATTLDEYRKHIEKDAALERRFQPV
                     FVAEPSVEDTISILRGLKERYELHHGVRIQDAAAIAAATLSNRYISDRQLPDKAIDLI
                     DEAAARLRTEIDSKPQLLDQVDRQIMQLEIEREALKKERDQASKDRLAGLNRELAELR
                     ERSGSLHARWETEKAAIGELQGVKAKIENTRVQIEAAERQADLEAAARLRYGTLRELE
                     AKLAEQEAKLKELQKAGALLKEEVEAEDVAAVVSRWTGIPVTRLIEGEMEKLVQMEDR
                     IHQRLVGQTEAVRAVSNAVRRARSGIQDTQRPIGSFIFLGPTGVGKTELARALAEFLF
                     EDERAMVRIDMGEYQEKHTVSRLVGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEIEK
                     AHPEVFNVLLQLLDDGRLTDGQGRTVDFKNTLIIMTSNLGSELWSDPNTTEQGTEEQV
                     RDRLARHFRPEFLNRIDEVVVFRRLTREQLALIVDLQLERVRTRLAEAGYRLEVSAEA
                     RAFLAEVGFDPLYGARPLKRAIQRELQDPLAMRILAGDLPAGATIQVERGADGLSFSV
                     EQPIRAAA"
     gene            3386..3775
                     /locus_tag="VI520_00950"
     CDS             3386..3775
                     /locus_tag="VI520_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLF36480.1"
                     /translation="MMRLYWGTPRHRANRDLIGFNGGQRLPIEVRADDEAYLITAEVP
                     GMSAEDLQVEVLEDLVTLRGKYKEDGAGEALINELERSGGFFRRIQLPEAIDAEAVEA
                     KVENGLLTVRVPKAEEARPKRIEVKAK"
BASE COUNT          636 a         1227 c         1209 g          705 t
ORIGIN      
        1 ggccaatggg ttggtcacgg gtgacgggtt ggcaggcttg cgctcgctct tgcagcccgc
       61 cgatctccgc cgaagggccg ccagagcacg cggccgtcgg ccgggttttg ggcccctagg
      121 tcgctgggca gtcttgcgga gagagtcgac tggtacggag agtgatctcg ccgggctcgc
      181 tgcccgccag ctgctgcgcc gctacggggt ggtgatccgg gaattgcttg cccgggagac
      241 ccgcattccg gcctggaggg cgctgctgag ggtctaccgt gaaatggagg cccgcggcga
      301 aattcggggc gggcgcttcg tggacggctt tgtcggcgaa cagttcgccc ttccggaagc
      361 agtcgacgag ctgcggcggg ttcgccgcga cacccagccg gaaccggccg tgatggtgcc
      421 ggcgtccgat ccgctcaatc tggtcggcat cttgaccccc ggaagccgga tttcgccgca
      481 ttccaatcag gtgattctct acgagcgggg tgtgccggtg gacgtcggcg aattgggcgc
      541 cgtgcgccac cggctgcggc gctcatccct ggctgagttt gaagcccgag aagtctgagt
      601 tcggatcagg ccgcggcccg aatcggctgc tcgacagaaa agctcagccc gtccgcccct
      661 cgttccacct ggatcgtcgc gcccgccggg agatcgccgg ccaaaatccg catggccagc
      721 ggatcctgca gctcgcgttg gatcgcccgt ttcaacggcc gggcgccata tagcggatcg
      781 aagccgacct cagccaagaa cgcccgcgcc tcggccgaga cctcgagccg gtagccggct
      841 tcggccaggc gggtccgcac ccgctccagt tgcaaatcga caatcagggc aagctgctcg
      901 cgggtcaggc ggcggaaaac aaccacctca tcgatccggt tcaggaactc cggacgaaag
      961 tggcgggcca ggcgatcgcg tacctgctcc tcggtgccct gttcggtcgt gttgggatcg
     1021 ctccagagct cgctccccag attgctggtc atgatgatca gggtgttctt gaagtccacc
     1081 gttcggccct ggccatcggt gagccggccg tcgtccagca gctgcagcag gacattgaag
     1141 acctccggat gggccttctc gatctcgtcc agcaggacca cgctatacgg gcgccgccgg
     1201 acggcttcag tgagctggcc accctcctca tatccgacgt agccgggcgg agcccctacc
     1261 agccgggaga ccgtgtgttt ctcttggtac tcccccatgt caatccgcac catggcgcgc
     1321 tcgtcctcga agaggaactc ggccagggcg cgggcgagct cggtcttgcc taccccggtc
     1381 ggtcccagga agatgaacga gccgatcggt cgttgggtgt cctggatccc ggagcgagcc
     1441 cgccgcaccg cgttcgagac ggcccgcacc gcctcggttt ggccaaccaa ccgctgatgg
     1501 atccggtcct ccatctggac cagtttttcc atctcccctt cgatcaagcg ggtcaccgga
     1561 atcccggtcc agcgggagac gaccgctgcg acgtcctcgg cttccacttc ttcctttaag
     1621 agggcaccgg ccttttgcag ctccttgagc ttggcttcct gctcggccag cttggcctcc
     1681 agttcgcgca gggttccgta gcggagccgg gccgcggcct ccagatcggc ctggcgctca
     1741 gcggcctcga tctgtacccg ggtattttcg atcttggctt tcacgccctg cagttcgccg
     1801 atggcggcct tctcggtctc ccagcgggca tgcagcgagc cggagcgctc gcgcagctcg
     1861 gcgagttccc ggttcaaccc agccagccga tccttggagg cctgatctcg ctccttcttg
     1921 agcgcctcgc gctcgatctc taactgcatg atctgacggt ccacctgatc gagcagctgt
     1981 ggcttggaat caatctcggt gcgcaaccgg gcggcagcct cgtcgatcaa gtcgatcgcc
     2041 ttgtccggga gctgccgatc gctgatgtag cggttggaga gggtggcggc cgcgatggcg
     2101 gccgcatctt gaatgcgcac cccgtggtgg agctcgtagc gctccttgag cccccgcaga
     2161 atactgatcg tgtcctccac gctcggttcg gcgacaaaga cgggttggaa gcgccgctct
     2221 agcgccgcat ccttctcgat gtgttttcgg tactcatcca gggtcgtggc accgatcagg
     2281 tggagctcac cccgggccag cagcggcttg agcatattgc cggcatccat ggcgccttcg
     2341 gctgctccgg cgcccacgac ggtgtgcatc tcatcgacga aaagcaggat ctcgccttcc
     2401 gcctcggtca cttcacgcag gactgccttc aaccgctctt cgaattcgcc gcggtacttg
     2461 gtaccggcca ctagcgagcc caggtccaat tggacgagcc gcttgcgctt gagcccctcc
     2521 ggcacatccc cccgcacgat ccgggccgcg agcccttcga caatggcggt ttttccgacg
     2581 cctggatcgc cgatcaaggc cggattgttc ttggtgcggc gagagaggac ctgaatcagc
     2641 cggcgaatct cctcatcccg gccaatcacc ggatcgagct tgccctgcct ggccagccgg
     2701 gtgagatcac ggccatactt ctccaaagcc tggtagctgt cctcgggggt cgggctgacg
     2761 acccgcgccg aaccccgcac gcttgtcagc gcttgcagga tggcgtccgg cgtcaccccg
     2821 aatttttcca gcagcttctg cagcggcccg gctgccaagg ccagcagcag gtgctcggcg
     2881 gagacgtatt cgtccttcat cccgctcgcc cgcagttcgg catccttgag aactgagttg
     2941 gtcgagcggg ccagtccgat ctgggaagtg gcaccgctga ccgtaggacg agcactcagc
     3001 gcggactcca actccgcctc gagcagctcg gtccctccga tgatccgagt gagcagggca
     3061 gggacgatcc cctccgcctg gcggagcagc gccaggagca ggtgctcggg ctccaggctt
     3121 gagtgcccgc ggtcctcggc cagccgttgg gcggcaagca atccactctg ggccttttgt
     3181 gtgtagcgtt caagctccat cgttttgttc ctttcaaatg ccatcgtaac ggcgaagagt
     3241 cggagcaaga taagaaccgc gtaagagcag gatcagcggg agggttaacg ggaatcctac
     3301 ggggaacata tcccattctg atgatcccca agtagagttg ctgcaattca ttcgccaaga
     3361 cagcgaacca aaaggagaga aggaaatgat gcgtctttac tggggcactc cgcgacaccg
     3421 ggcaaatcgc gacctgatcg ggttcaatgg cgggcagcgg ttgccaatcg aggtccgggc
     3481 cgacgacgag gcctacctga tcaccgctga ggtgccggga atgtcggccg aagatttgca
     3541 ggtcgaagtg ctggaagacc tggtcaccct gcgcgggaaa tacaaggaag acggtgcggg
     3601 cgaggctcta atcaacgagc tcgagcggtc cggcggattc ttccggcgga tccaattgcc
     3661 ggaggcgatc gatgccgagg cagttgaggc caaagttgag aacggcctgc tgacggtgcg
     3721 ggtcccgaaa gccgaggagg cccgcccgaa gcggatcgag gtcaaggcca agtaact
//
LOCUS       DATDPQ010000042         7357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3153363, whole genome shotgun sequence.
ACCESSION   DATDPQ010000042 DATDPQ010000000
VERSION     DATDPQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153363"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VI520_00955"
     CDS             <1..1081
                     /locus_tag="VI520_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HLF36481.1"
                     /translation="VATPAPPQAPTAGPTPDPGQASTPTPIPTSIPSRLLLTGIRHEY
                     QGWNNCGPATLAMALSFWGWIGDQRPVAQFTKPNPRDKNVMPYELADFVREQTDLEVV
                     VRVGGDLPLLKSLIAAGFPVMIEKGLLVPDVEGWMGHYELLAGYDESAGIFNAYDSYE
                     GDFGNGKTLAVSYQTVETYWRHFNHTYLILYPPERGRELFSMLGADADEQTNIRRAAE
                     RASEEIFRTTGRDLFFAWFNRGTNLMLLQDYGGAATAYDQAFKVYAELDPEVRPWRVM
                     WYETGPYFAYYYTGRYWDVIGLATQTLDNMSEPVLEESYYWRGLAKEALGDVAGAITD
                     YQSSLDFHPGFGPALTQLVRLGVAP"
     gene            1122..2684
                     /gene="murJ"
                     /locus_tag="VI520_00960"
     CDS             1122..2684
                     /gene="murJ"
                     /locus_tag="VI520_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HLF36482.1"
                     /translation="MNRPSIPVARSTLIVMGGMLASMAMGLVRQRVIAARFGTGVELD
                     AYTAANGLPELLFTMLAGGALTFAFIPIYSEFLTGDDRSDGNRLVSQVINSVFLLAVG
                     VSLLAAWAAPTLVSAPWGLGPHFPIEVQRLTADLMRVLLLSTILFAISSILSGVLHAH
                     QHFLLPAVSPIVYSLGVIFGALALSPSLGILGLGWGAVAGAALHLGVQLPGLIRYRVR
                     WSPGLGWTDPALRRVAVLMAPRVVDLMMARASIDWLNANLGSGLGEGRLSALRYAFQI
                     MNMPWSLVGTAIGIAVFPTFAALAAEQDRTALRGALSGALRAILTLTLPAAVGLLFLG
                     RPVIQVLFEGGEFTAESTDLVFYALRFYVLALISQSVLEVVVRAFASRKDTLTPLLVS
                     FFTTALNIGLAVTLARPERLSHGGLALANGVAVGVESLIGLAILQRRWGLLEVRRVVG
                     DLARSGLACAAMALAVTVGLRLAPEAPLAELILAGGLGTVVYFVAAAALGIREVRSLP
                     LTLLRSLQSRRA"
     gene            2685..3476
                     /locus_tag="VI520_00965"
     CDS             2685..3476
                     /locus_tag="VI520_00965"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLF36483.1"
                     /translation="MDRETIPPDKLFDFSGKVVLVTGAGRGIGTGIALRFAQAGAGVV
                     VSYRTSVAGARAVGEAIQALGVRALTLQADVRKKADVEALIARAVDGLGRLDILINNA
                     GPYPVAPLLEMSEAQWDEVLDSNLRGVHLCTQAAAAQMIRQGAGGAIVNIASIEAENP
                     AHGHSHYDAAKAGVVMHTRSAARELGPHRIRVNSVSPGVIWQEGIEEDWPDGVNRYLK
                     AVPLGRLGSFEDVADACLFLASPAARWITGANLVVDGGVMTNTVY"
     gene            3500..4012
                     /locus_tag="VI520_00970"
     CDS             3500..4012
                     /locus_tag="VI520_00970"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLF36484.1"
                     /translation="MLSADRIIELLDLRPLPLEGGHFRQSYRAAESISSDALPARYQR
                     DKQLCTAIYYLLTSDPDSFSALHRLPTDEIYHFYLGDPVEMLLLHPDGDSAFVRLGPD
                     LRAGQQVQFVVPAGVWQGSRLASDGACALMGTTMAPGYDEGDYTGGERGALSERYPDR
                     ADLIRELTRE"
     gene            complement(3999..5552)
                     /locus_tag="VI520_00975"
     CDS             complement(3999..5552)
                     /locus_tag="VI520_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36485.1"
                     /translation="MSEVVLRVEGITKRFPGVVANDGIDFELARAEIHGLLGENGAGK
                     TTLMNIVYGLYQPDEGKIFINGEPVEIKDPNDALARGIGMVHQHFMLVPVMTVCDNLM
                     LGHEVTRGLPLGLLSRVDRDRVFEEVKQLADAHGLAVNPDALVQDLSVGAQQRVEIVK
                     ALYRGADVLILDEPTAVLTPPETEELFSIMRSLVRQGKSIIFITHKLKEVLEIADRIT
                     VLRDGKVAGTTKPALTSEAELAAMMVGREVLLRVERGQAHPGEEVLVVQDLRVLDNRD
                     QPAVRGVGFSLREGEILGIAGVQGNGQSELVEALTGLRPAVSGRILLHGHDVTHAKPR
                     RITETGAAHVPEDRHKHGLVLVYPVDENLVLQTYYVSPFARGLNINPRAVRENARRLI
                     RDYDVRTPSIETRVGSLSGGNQQKVIVARELSRPIHLLIANQPTRGLDVGSIEYIHQR
                     IVAKRDEGVAVLLVSSELDEILSLSDRIAVIYGGEILAIVPAEEATRERVGLLMAGVR
                     EAAAELTPA"
     gene            complement(5563..6699)
                     /locus_tag="VI520_00980"
     CDS             complement(5563..6699)
                     /locus_tag="VI520_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36486.1"
                     /translation="MDVDFFVGLLSLALVKATPIAFGAMCGLMCERSGVINIGIEGMM
                     LVGAMMSFLGSVFFHNLTGQVLPVNLSLFVGLLVAMLSAGVIAALHAVLSIHYKVDQI
                     ISGTVINLLALGITNFVTSAYIDRVHLAGIGIFSPINVPLLSEIPIVGRILFMHQPLV
                     YVMLILVAVLQFALFNTPWGLRTRAVGEHPRAADTVGIKVLRTRYVNVILGGLLAGLG
                     GAFLVIQSVGRFEKFMTTGRGFIALAALIFGKWTSVGALGSTLLFGFSEALGVRLQFG
                     DVNELQTLIVLTGVGLLLLTLVMVGLNLARRSEDQRSFGLLAAVAMVGALLTAIGLFA
                     KVPTIVIPVQFLGLLPYVVTILVLAGVVGRAFPPAAIGKPYEKQ"
     gene            complement(6703..>7357)
                     /locus_tag="VI520_00985"
     CDS             complement(6703..>7357)
                     /locus_tag="VI520_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36487.1"
                     /translation="RIISWLIAPGGPMRASALNPVSETKPVLETARLPLLIADSRLHA
                     GVLLAPIAAVAIYWLLWRTTLGFEIRTVGANPSAARYAGINVGRNIVMTMMLSGAMAG
                     FAGGVQVVGLHPYNFTTGFNVGYGFDSIAVTMLGGLHPFGVVLASFLFGIMDAGGRFM
                     QLRTRAPIDILTIVQGLLLMFVAAKQIISGLYRIRARAEEGPVSFGRLWAGERGGAD"
BASE COUNT         1314 a         2434 c         2265 g         1344 t
ORIGIN      
        1 ggtcgcgact cccgcgccgc ctcaggcgcc gaccgccggt cccactcccg atcctggcca
       61 agcctcaacc cccaccccga tcccgaccag tattccctcc cgcctcctgc tcaccggcat
      121 ccggcacgaa taccaggggt ggaacaactg cggcccggcg accctcgcaa tggccctctc
      181 cttctggggc tggatcggag accagcgccc ggtggcacag ttcaccaaac ccaatccgcg
      241 cgataagaat gtgatgccct acgagttggc cgacttcgtc cgcgagcaga ccgacctcga
      301 ggtggtggtg cgggtgggag gcgatctccc gctgttgaag agtctgattg cagccggctt
      361 tccggtgatg atagagaagg gacttttggt gcccgacgtg gaaggctgga tgggacacta
      421 cgagctgttg gccggttacg atgagtctgc gggcatcttc aatgcgtacg attcctacga
      481 gggcgatttc ggcaacggta agactttagc ggtcagctac cagaccgtcg aaacctattg
      541 gcgccacttc aatcacacct acctgatcct ctatccgccg gagcgcggtc gggagctgtt
      601 ctccatgctc ggcgccgacg ccgacgagca gaccaatatc cgccgtgccg ccgagcgggc
      661 ctcggaggag atcttccgca ccactggccg cgatcttttc ttcgcctggt tcaaccgcgg
      721 aacgaactta atgctgcttc aggactacgg cggggccgcc acggcctatg atcaggcctt
      781 caaggtgtac gcagagctgg acccggaagt acggccatgg cgggtgatgt ggtatgagac
      841 cggaccgtac ttcgcctatt actacaccgg ccgctactgg gacgtgatcg gcctggcgac
      901 ccagaccctg gacaacatga gcgaaccagt cctggaggag agctactact ggcgcggtct
      961 ggccaaggag gccctgggcg atgtggccgg cgcgatcacc gactaccaga gcagcctcga
     1021 ctttcacccc ggctttggac cggcgctcac ccaactcgtc cggctcgggg tcgcccccta
     1081 agccgtgagc cggctgtccc gctgaaccag tcctcactgc aatgaatcgc ccttccattc
     1141 cggtcgcccg ctccacgttg atcgtgatgg gtggcatgtt ggcttcgatg gcgatggggc
     1201 tggtgcgcca gcgggtcatt gccgctcggt tcggcaccgg cgtggagctg gatgcctata
     1261 ccgcagccaa cgggcttccg gagctcttgt tcaccatgct cgccggcggg gcattgacct
     1321 tcgccttcat ccccatctac agcgaattcc tgaccggcga cgatcgttcg gacggcaacc
     1381 gcctggtcag ccaggtgatc aactcggtct tcttattggc cgtgggggtc tcgctgctcg
     1441 ccgcgtgggc cgccccgacc ctggtcagtg cgccctgggg gctcgggccc cacttcccca
     1501 tcgaggttca gcgattgacc gccgacctga tgcgggtact gctgttgtcg acgatccttt
     1561 ttgctatcag cagcatcctg agcggcgtac tgcacgccca ccagcacttc ctgctgccgg
     1621 cagtctctcc catcgtctac agcctggggg tgatcttcgg cgcgctggcg ttgtccccct
     1681 ctctagggat cctggggctc ggctggggag ccgtggccgg cgcggcgctc cacctcggag
     1741 tccagctgcc cggcctgatc cgctatcggg tgcgctggtc accgggcttg ggctggaccg
     1801 accctgccct gcgccgggtg gccgtcctaa tggccccccg agtggtggac ctgatgatgg
     1861 cccgggccag catcgactgg ctcaacgcca acctggggtc tgggctcggc gaggggcggc
     1921 tctccgcctt gcgctatgcc ttccagatca tgaacatgcc ctggtccctg gtgggcaccg
     1981 ccatcgggat cgcggtcttt cccacctttg ccgcgctggc ggccgagcag gaccgcaccg
     2041 cgctgcgcgg cgcactgagc ggcgcgctgc gggcgatcct gaccctgacc ttgccggccg
     2101 cggtcgggct gctcttcctc ggccggccgg tcatccaggt gctgttcgag ggcggggagt
     2161 tcacggccga aagcaccgac ctggtgttct acgcgctccg cttctacgtc ctggcgctca
     2221 tcagccagtc ggtgttggag gtcgtggttc gggccttcgc ctcccgaaag gacaccctca
     2281 ccccgctgct tgtgtcattt ttcacaaccg cgctcaacat cggcctggcg gtgaccctgg
     2341 cccgcccgga gcggctctcc catggcgggc tggcgcttgc caacggcgtg gcggtggggg
     2401 ttgagtcgct gatcgggttg gccattctcc agcggcgctg ggggctcttg gaggttcgcc
     2461 gggtggtggg cgacctggcc cggtccgggc tggcttgcgc cgcgatggcg ctcgccgtca
     2521 cggtcgggct gcggctggcg ccggaagctc cactggctga actcatcctg gccggcgggc
     2581 tggggacagt ggtctatttc gtcgcggcgg ctgccctcgg aatccgcgag gtgcgcagcc
     2641 tgccgctgac tttgctgcgc agtctgcagt cacggagggc ctgagtggat agagaaacga
     2701 ttcccccaga taagctgttc gatttctcag gaaaagtcgt gctggtgacc ggcgccggcc
     2761 gcggcattgg aaccgggatc gcgctccgct tcgcccaggc cggcgcgggg gtggtggtga
     2821 gctatcgaac gagcgtggcc ggcgctcggg ccgtgggcga ggccatccag gccttgggag
     2881 tacgagcgct caccctgcaa gcagatgtca ggaagaaggc tgatgtcgag gcgctcatcg
     2941 cacgggctgt cgacggcctg ggacgcctag acatactcat taacaatgcg gggccctacc
     3001 cggtcgctcc cctgctcgag atgagcgagg ctcagtggga cgaagtgctc gacagcaact
     3061 tgcggggcgt ccatctgtgc acccaggctg ccgcggctca gatgatccgg cagggagcgg
     3121 gcggggccat tgtcaacatt gccagcatcg aggccgagaa cccggctcac gggcacagcc
     3181 attacgatgc cgccaaggcc ggggtcgtga tgcacacccg ctcggcggcc cgggagctcg
     3241 gcccacacag gatccgggtg aactctgtgt cgccgggcgt gatctggcag gaggggatcg
     3301 aagaagattg gcccgacggg gtgaatcgct acttaaaggc tgtaccgctt ggcagattgg
     3361 ggtcgttcga ggatgtggcc gatgcctgcc tcttcctggc ctccccagcg gcccgctgga
     3421 tcaccggcgc caatctggta gtcgacggcg gggtgatgac caataccgtc tattgattca
     3481 gcatgcagcg ggaggcccga tgttgagcgc agaccggatc atcgaattac tcgacctgcg
     3541 gccgctgcca ctggagggcg gccacttccg gcaatcctat cgcgccgcgg aatctatctc
     3601 cagcgacgcc cttccagccc gctatcagcg ggacaagcag ctctgcaccg ccatctacta
     3661 tttgctgacc tctgatcccg actcattctc ggccctgcac cgtctgccta ccgatgagat
     3721 ctaccacttc tacttgggcg atccggtgga gatgttactg ctccacccag atggagacag
     3781 tgcattcgtc cggctcggcc ccgacctccg ggccggccag caggtgcagt tcgtcgttcc
     3841 ggcaggggtc tggcaaggtt ctcggctcgc ctcggacggc gcttgtgcct tgatgggcac
     3901 caccatggcg ccgggttatg acgaggggga ctacacaggt ggcgagcgag gggcgctgag
     3961 tgagcgctat ccggaccgcg ccgacctgat ccgcgaactt acgcgggagt gagctcggcg
     4021 gcggcctctc gcacgcccgc catcagcagc ccgacccgct cccgagtcgc ttcctcggcc
     4081 ggcacgatcg ccaagatctc gccgccgtag atcaccgcga tccgatcgga cagactgagg
     4141 atctcatcga gctccgacga cacaagtaag acggccactc cctcatctct tttcgcgacg
     4201 atccgttgat ggatgtactc gatcgatccc acatccagac cgcgggtcgg ctggttggcg
     4261 ataagcaggt ggataggccg gcttagctcg cgggcgacga tgactttctg ctgattgccg
     4321 ccagagaggc tgcccacccg ggtttcaatc gagggcgtcc gcacatcata gtctctaatg
     4381 agacgcctgg cattctcgcg caccgctcta ggattgatgt tcagccctct cgcaaaggga
     4441 gacacatagt aagtctgtaa gaccaggttt tcgtcaaccg gatagaccag caccaaaccg
     4501 tgcttgtgcc ggtcctcggg gacgtgcgcc gcacccgttt cggtgatgcg ccgcggcttg
     4561 gcgtgggtta catcgtgccc gtgcaacaaa atccggccgc tcacggccgg gcgcaggccg
     4621 gttagcgcct cgaccaattc ggactgccca ttaccctgaa ccccggcgat gccgaggatt
     4681 tcgccctcgc gcaagctaaa tccaacgcct cgcactgccg gttggtcacg gttgtccagc
     4741 acacgcagat cctgcaccac caggacttcc tcaccgggat gggcttgtcc tcgctcgact
     4801 cgcaagagca cttcccggcc caccatcatg gcggctagct cggcctcact ggtcagggca
     4861 ggtttggtcg tgccggcgac cttgccgtcc cgcagcacgg tgatccgatc ggcgatctcc
     4921 agaacttcct tgagcttgtg ggtgatgaag atgatggatt tgccttgcct gacgagcgag
     4981 cgcatgattg aaaacaactc ttccgtctca ggaggagtca gcaccgcggt cggttcgtcg
     5041 aggatcagta catccgcgcc ccggtagagc gccttgacga tctcgactct ctgttgcgcg
     5101 cccaccgaca ggtcctgcac cagggcgtct gggttcaccg ccaggccgtg cgcgtcggcc
     5161 agctgcttga cttcttcgaa cacgcggtca cggtccaccc gcgacaagag ccccaaaggc
     5221 aaaccccggg tgacttcatg gcccagcatc aggttatcgc acaccgtcat gaccggcacc
     5281 agcatgaagt gctggtgaac catgccaatc ccccgggcca gtgcgtcgtt cgggtctttg
     5341 atttcgaccg gctcgccatt aatgaagatc ttcccctcat ccggctggta caggccgtag
     5401 acgatgttca tcagggtcgt cttgccggca ccgttctctc ccagcagccc gtggatctcc
     5461 gcgcgtgcca gctcaaagtc gatcccatca ttcgccacca cgccggggaa gcgcttggtg
     5521 atcccctcga cccgcaggac cacttcgctc attgcgccgg actcactgtt tctcataagg
     5581 cttgccgatg gcggcaggcg ggaacgcccg cccaacgact ccggccaaaa ccaggatggt
     5641 gacgacgtag ggcagtagcc cgaggaactg aaccgggatg acgatcgttg ggacctttgc
     5701 aaagagacca atggccgtca agagcgcgcc aaccatcgca acagctgcga ggaggccaaa
     5761 ggatcgctga tcctcgctgc ggcgcgccag gttgagcccg accatcacca aagtcaggag
     5821 cagcagaccc acgccggtga gcacgatcag cgtctgcaat tcattcacat ctccaaactg
     5881 caggcgcacg ccgagcgcct cgctaaagcc gaacagcagg gtcgagccga gtgcgcccac
     5941 cgaagtccac tttccgaaga tcagcgcggc cagagcgatg aagccgcggc cggttgtcat
     6001 gaacttctcg aagcgcccca cgctttgaat caccaggaac gcgcccccca atccagccag
     6061 caacccaccc aggatgacat tgacatagcg ggtacgcaac actttgatgc caacggtatc
     6121 cgccgcgcgg gggtgttccc cgacggcacg ggtgcgcagg ccccacggcg tgttgaagag
     6181 ggcgaactgt aacaccgcca ccaggatcag catcacgtag accagcggct gatgcatgaa
     6241 gaggattcgg cccacgatcg gaatctcgga taacagcggg acgttgatcg gcgagaagat
     6301 acctatcccg gccagatgca cccggtcgat gtaggcgcta gtgacgaagt tggtaatccc
     6361 cagcgccagc aggttgatca cggtcccact gatgatctgg tccaccttgt agtggatcga
     6421 gagcaccgcg tgcagagcgg cgatcacgcc cgcgctcagc atggcgacca acagcccgac
     6481 aaacaggctc aggttgactg gcagcacttg accggtcagg ttatgaaaga ataccgaccc
     6541 caggaatgac atcatcgcgc cgaccagcat catcccttcg atgccaatat tgatcacgcc
     6601 cgagcgttcg cacatcaatc cgcacattgc gccaaatgcg ataggtgtgg ccttgaccag
     6661 agccagggag agcaggccaa caaagaaatc cacgtccatg gcttagtccg cgcctcctct
     6721 ctccccggcc cacagccgcc cgaagcttac cggtccctcc tcagcccggg cgcgaatgcg
     6781 gtagagacca ctgatgatct gcttcgccgc cacgaacatc agcagcaggc cttgcacgat
     6841 cgtgaggatg tcaatcggag cccgggtgcg cagctgcatg aaccgccccc ccgcatccat
     6901 gatgccaaaa aggaaagatg ccagcacgac cccaaaggga tgcaggcccc ccagcatcgt
     6961 cactgcgatg ctgtcgaatc cgtatccgac gttgaagccg gtcgtaaagt tgtacggatg
     7021 gagccccact acttgcacgc caccggcgaa gccggccatc gccccgctga gcatcatcgt
     7081 catgacgatg ttgcggccga cattgatccc ggcataacgc gccgcgctcg gattggcccc
     7141 cactgtacga atctcaaatc ctaaggtcgt tcgccacaac aaccagtaga tcgcaaccgc
     7201 tgcgattggc gcaagcagca cccccgcatg caagcggctg tcggcgatga gcagcggcag
     7261 gcgagcggtt tcgaggaccg gtttggtctc ggagaccgga ttcagcgcgc tggcccgcat
     7321 cgggccgccc ggggcgatca gccagctgat gatgcgc
//
LOCUS       DATDPQ010000043         2606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3415495, whole genome shotgun sequence.
ACCESSION   DATDPQ010000043 DATDPQ010000000
VERSION     DATDPQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3415495"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VI520_00990"
     CDS             <1..1218
                     /locus_tag="VI520_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLF36488.1"
                     /translation="EDYTLAVLFLDLDRFKVVNDSLGHMTGDQLLVGVARRLETCLRQ
                     VDTVARLGGDEFVVLLEDIENVGHATATAERIQEQLQENFRLDDNDVFTSASIGIAVS
                     KTGQDRAQDILRDADIAMYRAKSMGKARYALFDETMHGRAVELLQLETDLRKAVDREE
                     FLLHYQPIVSLLSGEIVGFEALVRWMHPERGLTFPAAFVPLAEETGLSIPMGEWVLRN
                     ACRQLQTWRAALPNERSLWVSVNLSSRQLLQPGLIKLVESCLRQAQSDPATLHLEVTE
                     GVIMDEPEAATAMLVGLRELGVGLPIDDFGTGYSSLAYLHRFPITSLKIDRSFVKRVA
                     SGGEDEVIVRTIVSLAHNLGMDVIAEGVETAEQLERLTALGCEFGQGFYFSKPVPAEE
                     ASQLLKLPVAVLA"
     gene            1274..2356
                     /locus_tag="VI520_00995"
     CDS             1274..2356
                     /locus_tag="VI520_00995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HLF36489.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAVVKSADQHGGYEVLGFKDGFEG
                     CLDPEGVVELSLDAVHGILPRGGTILGAANRGNPFARKVVRDGLEVMEDVSDQVIRRI
                     EQLELESLIVVGGDGTLHISLELFHKGMPIIGIPKTIDNDIEGTDVTFGFDTAVNTAT
                     EALDRLHTTAEAHHRVIVLELMGRDAGFIALHAGVAGGADVILIPEIPFEFKGICETI
                     QARASRGRHFSTVAVAEGARPKGGEQVFRKAGDELVTARLGGIGQTVAEYVEEVCGME
                     ARVMVLGHLQRGGTPTAFDRWLATRFGAAAFRLAVQRTHGRMVALRGVSVVDLALSEA
                     LAVPKRVDLNGDGVRTARTLGLSLGD"
     gene            2359..>2606
                     /locus_tag="VI520_01000"
     CDS             2359..>2606
                     /locus_tag="VI520_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36490.1"
                     /translation="MDNNAPDSLRVFAIGFLLAAGFFLIEAGAAEVMLARRADCVEQL
                     GRMRLAPNPEQACMSEFQYFLTRAVSRGVFVSTDPEPSG"
BASE COUNT          470 a          735 c          913 g          488 t
ORIGIN      
        1 gaggattaca ccctggccgt gctgttcctc gacctggatc gcttcaaagt ggtcaacgac
       61 tccttgggtc acatgaccgg cgaccagctg ctggtcgggg ttgctcgccg gctggagacc
      121 tgtctgcggc aggtggatac cgtggcgcgc ctgggtggcg acgaattcgt ggtcttgctg
      181 gaggatatcg aaaacgtcgg ccacgccacc gcgaccgcgg agcgaatcca ggagcagctg
      241 caggaaaact tccgtttgga tgacaacgac gtcttcacca gtgccagcat cgggatcgca
      301 gtgagcaaga ccggccagga tcgggcccag gacattttgc gcgatgccga tattgcgatg
      361 taccgggcca agtccatggg caaagcgcgc tatgcgctgt ttgacgagac gatgcacggt
      421 cgagcggtcg aactgctcca actggaaacc gacctgcgca aagcagtaga tcgagaggag
      481 tttctgctgc actaccagcc gatcgtgtcg ctgctctccg gcgagatcgt cggtttcgaa
      541 gcgctggtcc gctggatgca tccggagcgg gggctgacct tcccggctgc ctttgtgccc
      601 ctggccgagg agaccgggct ctccattccg atgggtgagt gggtcctgcg caatgcctgt
      661 cgccaactgc agacctggcg agcagccctc cccaacgagc gcagcctgtg ggtcagcgtg
      721 aatctctcca gccgccagct gcttcagccc ggattgatca aactcgtgga gagctgcctg
      781 cggcaagccc aatccgaccc ggccactctg cacctggaag taaccgaagg cgtcatcatg
      841 gacgagccgg aagcggccac cgccatgctg gttggcttgc gggagctggg ggttgggctg
      901 ccgatcgatg actttggcac cggatactca tcgttggcct atctgcaccg cttcccgatc
      961 acttcactga agatcgaccg cagcttcgtc aagcgggtcg cctcgggagg ggaggacgag
     1021 gtgatcgtgc gaacgattgt gagtctcgcc cacaatctcg ggatggatgt gatcgccgag
     1081 ggcgtggaga ctgccgagca gctcgagcgg ctcactgcgc tgggttgcga gttcgggcaa
     1141 gggttttact tctcgaagcc agttcccgcc gaagaagcat cgcagctgct taagctcccg
     1201 gtcgcagtcc tagcctgacc gggtagaatc ctccactcca gagactcgga ttgtcagctc
     1261 aatgaggtga aggatgaagc gaatcgggat tctcaccggc gggggcgacg cgcctggact
     1321 caatgcggtg attcgggcgg tggtgaagtc ggccgaccag catggcggtt acgaagtgct
     1381 cggattcaag gacgggttcg aaggctgcct tgacccggag ggggtggtgg agctcagtct
     1441 ggacgcggtc catgggattc ttccccgcgg cggcacgatc ttgggagcgg ccaaccgggg
     1501 caacccgttt gccaggaagg tggtccggga tggcctggaa gtaatggagg atgtctcgga
     1561 ccaggtcatc cgccgaatcg agcagcttga actggaaagt ttgattgtgg tgggcggaga
     1621 cggtacgttg cacatctcgc tcgaactctt ccacaaggga atgcccatta tcgggatccc
     1681 gaagacgatc gacaacgaca ttgaggggac cgacgtgacc ttcgggtttg acacggcggt
     1741 caacacggcg accgaggcgc tcgaccggct gcacacgacg gcggaagccc accaccgggt
     1801 gatcgtgctg gaactgatgg ggcgggacgc cgggtttatc gccttgcatg cgggcgtcgc
     1861 agggggagcg gacgtaatcc ttatcccaga aatcccattt gagttcaaag ggatttgtga
     1921 gacgatccaa gcccgcgcca gccggggcag gcacttcagc accgtggcgg tggccgaagg
     1981 cgcccgtccg aaaggcggag agcaggtgtt ccgaaaggcg ggcgacgagc tggtgacggc
     2041 ccgcctgggc ggcatcggcc agacggtcgc ggaatatgtg gaagaggtgt gcgggatgga
     2101 ggccagggtg atggtgctgg gtcatctgca gcggggcggt acgccgacgg ccttcgaccg
     2161 ctggctggcg acccgctttg gggcggccgc ctttcgatta gccgtgcagc ggactcacgg
     2221 acggatggtt gcgctgcgcg gagtgagcgt tgtggatttg gcgctgagcg aggcgctcgc
     2281 ggtgccaaag cgggtggacc tgaatggcga cggggtgcga acggcccgaa cgctggggct
     2341 cagcctgggc gactgaccat ggataataac gctcccgact cactgcgggt cttcgcaatc
     2401 ggcttcctac tggccgcggg gttcttcttg attgaggccg gcgcggccga ggtcatgctg
     2461 gcccggcggg cggactgcgt cgagcagttg ggtcgaatgc ggctggcgcc gaatccggag
     2521 caggcctgca tgtcggagtt tcagtatttc cttactcggg cagtctcccg tggggtgttt
     2581 gtttcgactg acccggagcc gtcggg
//
LOCUS       DATDPQ010000044         5849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_798303, whole genome shotgun sequence.
ACCESSION   DATDPQ010000044 DATDPQ010000000
VERSION     DATDPQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798303"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VI520_01005"
     CDS             complement(<1..744)
                     /locus_tag="VI520_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLF36491.1"
                     /translation="MATEHPEFDLVTMGRASLDLYSNDVGAPFSEITSFAAYVGGSPT
                     NIAVGARRLGLRAALLTALGADLVGDFVLNFLEAEGVSTEFIPRKPGTRTSAVLLGIE
                     PPDRFPLVFYRENAADVQLTIDDVLAAPIEKALAFEFAGTNLAKEPSRSATFFAAERA
                     SRAGAKVFMDLDFRADQWHDIRAFGLAVRAILKHVHVVVGTEQEIKATALEQQDQLVI
                     QHSQLSEPEVVGQLDPAARMLLEQGPGAVV"
     gene            complement(808..1704)
                     /locus_tag="VI520_01010"
     CDS             complement(808..1704)
                     /locus_tag="VI520_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLF36492.1"
                     /translation="MIKVANAPVSWGVIEKVEGERSAYARVLDEIAEAGYAGTELGDW
                     GFLPTDSDLLATELEARGLELMAAWVGVAYADKLAHADGEARAVQAARLLAKVAGRQA
                     VIVLGEDPSPQSHRSQYAGRIQPEHALSGNGWEIYAEGVNRIARAVKEATGLRSAFHP
                     HSGIWIETQEETERLLSSTDPALVGLCFDTGHYRFGGGDPVAALRQFADRLWHVHFKD
                     QDPQVAARSRAEQWDYDRSIAEGIFCELGRGDVDFPGALEVLQEIGYDGWIVVEQDVM
                     PGSGSPKESARKSRDYLRSLGI"
     gene            complement(1701..2852)
                     /locus_tag="VI520_01015"
     CDS             complement(1701..2852)
                     /locus_tag="VI520_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLF36493.1"
                     /translation="MARLRVGVIGCGTIAQIMHLPYLRQLNDLYEISALCDVSRQVLE
                     AMGEHYGVERRYTDYHELLMLDLDAVLLLIPGSHAPAALAALRSGKHVLVEKPMCFTL
                     AEADQLIEAAESSGRTLMVGYMKRYDPGYRHAQTSLPRLGELRYIQINTLHPAEEPFL
                     NVHRLVRAGDLPAETLRQLASEKDRLVSEAIGPVSQPMKQIYTEVLLGSLVHDINALR
                     GLVGEPQAVEYTAIWPADEVLPVITTVLRVRDQLRAAFVWAYLPDYHDYFEEIALMSA
                     GGRIRIQFPSPYLRHFPTPIVIEGMQDGAAVKQHITASYAEAFKEQLLHFHRCVTTGE
                     RPLTDARDARKDIEILQQVLAAANPPGLGGEAIRLRAQDTQRRTEQEAR"
     gene            complement(2855..3610)
                     /locus_tag="VI520_01020"
     CDS             complement(2855..3610)
                     /locus_tag="VI520_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF36494.1"
                     /translation="MDNSETPVVYMENIVKRFGTVTALDGVDFTVHPREVAALVGDNG
                     AGKSTLIKILTGVHTPTRGQIYFEGKPVQIRSPQDSRALGIETVYQDLALVDLMSITR
                     NFFLGRELVGGIGPLRFLKLRRMNQQTRTSLSDIGIEVRSPTEKVERLSGGERQSIAI
                     GRALHFGAKLLILDEPTSALSVGETRKVLNYTLNAKERGLSVIFITHNIHHVYMVADR
                     YTIIRHGKLVGNYLKSELTETDIADLITGVREA"
     gene            complement(3649..4653)
                     /locus_tag="VI520_01025"
     CDS             complement(3649..4653)
                     /locus_tag="VI520_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36495.1"
                     /translation="MRRAAPWPHPLRRALADSPILGPLLTLVVVFILCSAFVPNFLTL
                     RSISGIISAATLTGTMAIGVTLLMIGGEFDLSVGALVAVAGYLYAFNTVDGGSPGIAL
                     ILAISIPAALGALNGLILIATRIPSFIATLGTRSIYRGLLWVLSTGALVQTLEDLPIY
                     RLFNGRLDVLNQLAEGANFRTALLWLVGLVIVFHWILVRTDFGSHVFAVGGNPGAAKA
                     QGVNVKLVKVVTFAIGGALAGFTGVLLFSQFKSVRVTTGAGMELSAIAAAVVGGALLS
                     GGSGSIWGSLIGVLLISTLRTGVVLLDLPFIPADNFEAVVGATIVGATILNKWLRSRG
                     "
     gene            complement(4801..>5849)
                     /locus_tag="VI520_01030"
     CDS             complement(4801..>5849)
                     /locus_tag="VI520_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLF36496.1"
                     /translation="TLFGIDQQPYLQGYSAVVWLFMTNRFGITPALPVSATGPGFVDA
                     SNVGTTPDPDREVTLLMVQHATCSWDAYWCVVENGIKLAAEQMGITTTVLAPDSFDLE
                     RTAALIDQAVAASPDGIALTVTDPILFKEPIERALAAGIPVVAYDSGAGPIKDNIAYL
                     TFLGADEYQGGFQSGTRLAGQPGAEVGVCINHQVGHTGLDARCQGFVDSMTEKGLTAE
                     VLAITNDPAQSQTIISDFFTANPQVNVALTLGPNGATPFYSFLEASGLGPGDIAHGTF
                     DLSEEIVAAIDDGRTLFAVDQQPFLQGYGAVMLLNLLLRQGVTPALPVTPTGPGFVDQ
                     SNIDIVKALAGQYR"
BASE COUNT         1151 a         1842 c         1762 g         1094 t
ORIGIN      
        1 caccacggcc cccggcccct gctcgagcaa catgcgggct gcgggatcca gctggccgac
       61 cacctccggc tcgcttaatt gggaatgctg gatcaccagc tggtcttgct gctcgagggc
      121 tgtcgctttg atctcctgct ctgtgccaac cacaacgtgc acgtgcttca ggatggctcg
      181 gacggccagg ccaaaggcgc gaatgtcgtg ccactggtcg gcccgaaaat ccaggtccat
      241 gaaaaccttg gcacctgccc gacttgcgcg ttcggccgca aaaaaagtcg cacttcggct
      301 tggttccttg gccaggttag tgcctgcaaa ttcaaatgcc agcgctttct caattggggc
      361 ggccaagacg tcatcgatag tcaactggac gtctgccgcg ttctcacgat agaagaccag
      421 cggaaaccga tcgggtggct cgattccaag cagcaccgcg ctggtacggg tacccggctt
      481 gcgcggtatg aattcagttg acactccttc tgcctcgagg aaattgagca cgaagtcgcc
      541 gaccaggtct gctcccagag cggtaagcag cgcggctcgc aacccgagcc ggcgggcgcc
      601 cacggcaatg ttcgtcggcg agccgcccac ataggccgcg aagctcgtga tttcactaaa
      661 gggcgccccg acgtcgttgc tatacagatc aagggaggcg cgccccatag tgacgagatc
      721 gaattcagga tgctcggtag ccacgtctag cctgctcgtt cgccggtctg gaagttcacg
      781 ttctggactc cactgaagcc ggtggactca tattcctagg ctgcgcaggt agtcccggct
      841 ctttcgggcg ctttcctttg gactgccaga cccgggcatc acatcttgct ctacgacgat
      901 ccagccatcg tagccgatct cctgcaacac ctctaaggcg cccgggaaat ccacatctcc
      961 ccgcccgagc tcgcagaaga tcccctccgc gatcgaacgg tcgtagtccc actgctcggc
     1021 ccgagagcgg gccgccacct gagggtcctg atccttgaag tgcacgtgcc acaggcgatc
     1081 cgcgaactgc ctcagcgctg ccaccggatc gcccccacca aaacgatagt gaccggtatc
     1141 gaagcacagg ccgaccaggg ccgggtcggt cgaacttagc agcctctcgg tctcctcctg
     1201 ggtttcaatc caaattccgc tgtgaggatg aaaggccgat cgcaagccgg ttgcttcttt
     1261 caccgcccga gcgatgcggt tcacaccttc agcatagatc tcccagccgt tcccgctcaa
     1321 ggcgtgttct ggctgtattc gaccagcgta ttgactgcga tgcgactgag ggctggggtc
     1381 ctctcccagg acgatcacgg cctggcggcc ggctaccttc gccaacaatc gggccgcctg
     1441 caccgcccgg gcctcgccat cggcatgcgc gagtttatcc gcatacgcaa cgcccaccca
     1501 ggcggccatc agctcgagac cgcgggcctc cagttctgtc gccagcaggt ccgaatcggt
     1561 ggggaggaat ccccagtcgc ccaactccgt gccggcgtag ccggcctcgg cgatttcgtc
     1621 cagtacccgg gcataggcag atcgttcgcc ttccaccttt tcgatgactc cccacgacac
     1681 gggggcattt gcgaccttga tcacctggcc tcctgctcgg ttcgccgctg agtgtcctga
     1741 gcccgcaatc tgatggcctc gcccccgagt cctggcggat tggccgcggc caggacctgc
     1801 tggaggattt cgatgtcttt gcgggcgtcg cgggcgtcgg tgagcggccg ctcccccgtt
     1861 gttacgcatc tgtgaaagtg caacagctgc tccttgaagg cttcggcgta agaagcggtg
     1921 atgtgttgtt tcacggcggc accgtcttgc atcccttcga tgacgatcgg agttggaaaa
     1981 tggcgcagat aaggtgaggg gaactgaatc cggatgcggc cgccggcgct catcagcgcg
     2041 atttcctcga agtagtcgtg ataatccggc aggtaggccc aaacgaaggc ggccctcaac
     2101 tgatcgcgca cccgcaatac ggttgtaatc acgggcagta cctcgtcggc gggccagatg
     2161 gcggtgtact ccacagcctg cggctcgccg accagccctc gcagtgcatt gatatcgtgg
     2221 accagactcc ccaagagcac ctccgtgtag atttgcttca tcggctgcga gaccggcccg
     2281 attgcctcgc ttaccagccg atccttctcg gacgcgagct gtctgagcgt ttccgccggc
     2341 aaatcgccgg cccgcaccaa gcggtgcacg ttgagaaacg gctcctcggc gggatgcagg
     2401 gtattgattt ggatgtaccg aagttcgccc agtctcggca gtgaggtctg ggcatgccgg
     2461 tagccggggt cataacgctt catatagcca accatcagag tgcgcccact ggactcggcc
     2521 gcttcaatca attgatcggc ctccgccagg gtaaagcaca tcggcttctc caccaggaca
     2581 tgtttgccgc tcctcagtgc agccagcgcc gcgggggcgt gcgagccggg gatgagcagg
     2641 agaacggcgt ccaaatccag catgagcagc tcgtggtaat cggtgtatcg ccgctcgacg
     2701 ccgtagtgct ctcccatggc ttccaggacc tggcgggaaa catcgcacag agcgctgatc
     2761 tcatacaaat cattcaattg cctgagatag ggcaggtgca tgatctgggc gatcgttcca
     2821 caaccaatca caccaacccg tagccgcgcc atggctatgc ctcgcgcact ccggtgatca
     2881 agtcggcgat atcggtctcg gtgagctcgc tcttcaggta gttgccgacc agcttgccgt
     2941 gacggatgat ggtgtatcgg tccgccacca tatacacatg gtggatgttg tgggtgataa
     3001 agatgaccga aagccctcgc tccttggcgt tcaaggtgta attgaggacc ttccgggttt
     3061 cgcccacgga aagcgccgag gtgggttcat caagtatcag aagtttggcg ccaaaatgaa
     3121 gcgcccgacc gatggcaatc gactgccgct cgcctcccga aagcctttca actttctcgg
     3181 tcggcgagcg gacctcgatc ccgatatcgc tcaatgaagt gcgggtctgt tgattcatgc
     3241 gccgcagctt aagaaagcgg agcggtccga ttccgcccac cagctcacgg cctagaaaga
     3301 agttacgggt gatgctcatc aggtcaacca aagcgagatc ctggtaaacg gtctcgatcc
     3361 caagggcgcg ggaatcctgg ggggagcgaa tctggaccgg cttcccttca aagtagattt
     3421 ggccgcgggt cggggtatga acgccggtga ggatcttgat cagggttgac ttgcccgcgc
     3481 cattgtcgcc caccagggcg gcgacctccc gcgggtggac cgtgaaatcc actccgtcga
     3541 gggcggtgac ggtgccaaaa cgcttgacga tgttttccat gtagaccacg ggtgtttcac
     3601 tgttgtccat cccggcctgc tcctcgatat gggcgggcta ccgcgaaact aaccccggct
     3661 gcgaagccat ttgttgagaa tggttgcgcc gacaatcgtg gcccctacga cggcttcgaa
     3721 attgtccgcc gggatgaaag gcaagtcgag caataccaca cccgtccgca acgtactgat
     3781 caacaggacg ccgatcagcg atccccagat gctgccggag cccccactca acagggcccc
     3841 tccgaccacc gccgccgcaa tggctgacaa ttccatgccg gccccggtcg tgacccgcac
     3901 gctcttgaac tggctgaaga gcaagacccc ggtaaagccg gccagtgcac ccccaatggc
     3961 aaaggtcacc actttgacca gctttacatt gaccccctgg gccttggccg cccccggatt
     4021 ccctcccacg gcgaacacgt ggctcccgaa gtccgtgcgg accaggatcc aatggaaaac
     4081 gatcaccaac ccgacaagcc agagcagggc cgtgcggaag ttggcgcctt cagcgagctg
     4141 attcagcaca tccaatcggc cgttgaacag ccggtagatg ggtaggtcct ccaaggtttg
     4201 gacgagggcg ccagtggaga gcacccacag caaacctcgg tagatggagc gggttcccaa
     4261 ggttgcgatg aacgatggaa tccgggtggc gatcaggatc agcccgttga gggcccccag
     4321 ggcagcaggg atgctgatcg ccaggatcag cgcaattccc ggtgatccac cgtcgacggt
     4381 attaaaggcg tacaggtatc cggccaccgc aaccagcgcg ccgaccgaca gatcgaattc
     4441 ccctccgatc atcagcagag tgactccgat cgccatcgtg ccggtaaggg tggcggcact
     4501 gataatgccg gagatcgaac gcagagtcag gaagtttggc acgaaagccg aacatagaat
     4561 aaagaccacg acgagcgtga gcagcggccc gaggatgggc gaatccgcca gcgcccgccg
     4621 cagcgggtgg ggccagggag ccgctcgtct cactgaggca gcaatttcca tattctcctt
     4681 ggcggaaggt ggagcttagc gggagggagg agcagcgctc ctccctccca ccagccgaac
     4741 gatagaggcg ggggatcgag cgtcaaacca gcagcccgct cgccacccac gccttgtgga
     4801 ctagcggtat tgacctgcca aggccttgac gatgtcgatg ttgctctgat cgacaaaacc
     4861 tggcccggtc ggtgttaccg gaagtgccgg cgtgacccct tggcgcagca gcagattcag
     4921 cagcatcact gcaccatacc cttgcaggaa cggctgctga tccaccgcga agagggtcct
     4981 gccatcgtcg atggcggcga cgatctcttc gctcaggtcg aacgtgccgt gcgcgatgtc
     5041 ccctggccca agaccagagg cctccaggaa ggagtagaac ggggtggccc cattcggccc
     5101 gagggtcagc gctacgttga cctgcgggtt ggcggtgaag aagtcgctga tgatcgtctg
     5161 ggattgtgcc gggtcattgg tgatcgccaa cacttcggcg gtcagaccct tctctgtcat
     5221 cgagtctacg aagccctggc agcgagcgtc cagtccggta tgacccacct gatggttgat
     5281 acataccccg acctcagccc ctggctgtcc tgccagccgg gtgccactct ggaacccgcc
     5341 ctggtactcg tccgcaccaa ggaaggtcaa gtaggcgatg ttgtctttga tcggcccggc
     5401 accggagtcg taggcaacta ccgggatgcc ggcggcgagc gcgcgctcga ttggctcctt
     5461 gaagagaatg ggatcggtca cagtcagggc gatgccatcg gggctggccg ccaccgcctg
     5521 gtcgatcagg gcggctgtcc gctcgaggtc gaacgaatcg ggggccaaga cggtcgttgt
     5581 gatccccatc tgctcggccg ccagcttgat gccgttctca acgacgcacc agtaggcgtc
     5641 ccaggagcag gtggcatgct gaaccatcaa gagggtgacc tcgcggtcgg gatccggggt
     5701 ggtgccaacg ttgctggcat ccacgaatcc gggccccgtg gcactcaccg gcagcgccgg
     5761 ggtgatgccg aagcggttgg tcatgaagag ccacaccacg gcgctgtaac cctgcaagta
     5821 cggctgctgg tcaatcccga acagggtta
//
LOCUS       DATDPQ010000045         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3154231, whole genome shotgun sequence.
ACCESSION   DATDPQ010000045 DATDPQ010000000
VERSION     DATDPQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154231"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VI520_01035"
     CDS             <1..464
                     /locus_tag="VI520_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HLF36497.1"
                     /translation="LQIVHIEDEPEPTYAELLAAGLYPGMELRVVERLATQMRIRVGR
                     DEHILRSPVAANLLVQPQPGTAAQAPQARLSDLRVGERGAVVGIAPGCRQMERRRLQD
                     LGVLPGTVIEAEFASPGGDPVAYRIRGATIALRKEQAAHILVDSLEESLCA"
     gene            491..2479
                     /gene="feoB"
                     /locus_tag="VI520_01040"
     CDS             491..2479
                     /gene="feoB"
                     /locus_tag="VI520_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HLF36498.1"
                     /translation="MHQAADPSTLGVDRQRWDYVVALGGNPNTGKSTVFNALTGLRQH
                     TGNWPGKTVARAEGGYEYAGLHFKLVDLPGTYSLLATTVDEQIARDFILSGQPDVTVI
                     VVDATRLERNLNLVQQVLEITDRVVVCLNLMDEARRHGLAVDDRQLTRDLGVPVVPTV
                     ARRGRGLDRLMSAIAEVAGGKLVGRTPRIASPESQATREAALSSVSKTDGNRHGQSVE
                     ATYAEAARLAGRAVRQANTQPRFDLDRAIDRIVTSRIWGFPIMLTLLAGVFWITITGA
                     NVPSRLLGTFLLDTVHPWLREAAADLGVAAWLRGVLIDGMYLATAWVVSVMLPPMAIF
                     FPLFTLLEDFGYLPRIAFNLDNLFKRAGAHGKQALTMAMGFGCNAAGVISARIIDSPR
                     ERLIAILTNNFALCNGRWPTQILIATIFLGTLVPARLAGAVSALAVVGVAILGVGLTF
                     AVSWLLSRTMLRGEASAFSLELPPYRPPQVLKTLYRSVIDRTVYVLWRAVVFAMPAGA
                     VIWIVANLQLGGISLAERLVDLLNPVGLLLGLNGVILLAFMAAIPANEIVVPTILMLT
                     VLAGGLSGVGVGAGVMFQLESGAGTLAILRAGGWTLLTGVNVMLFSLLHNPCSTTIAT
                     IYKETGSKRWALISAVLPLGIGLLLTGVVAQLWRLVAA"
     gene            complement(2481..>2757)
                     /locus_tag="VI520_01045"
     CDS             complement(2481..>2757)
                     /locus_tag="VI520_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36499.1"
                     /translation="PGRANQMGKLEQRITDWIKTYEEIVICGHTHRPAFPKIGEIPYF
                     NTGSGVVPGILTGLELRHGALVPIRWTEDYARRPVRELAGPPRRFNW"
BASE COUNT          424 a          884 c          931 g          518 t
ORIGIN      
        1 ccttgcagat tgtccacatc gaggacgaac cggagccaac ctacgccgaa ttgctcgcgg
       61 ccgggctgta cccggggatg gagctgcggg tggtggagcg gttggcgacc caaatgcgga
      121 tccgggttgg ccgggacgag cacatcttgc gctctccggt ggccgccaat ctgctcgtac
      181 agcctcaacc gggaaccgcg gcgcaggccc ctcaagcccg gttgagcgac ctgcgggtcg
      241 gcgagcgcgg tgcggtcgtg ggtattgccc caggctgtcg ccagatggaa cgccgtcgct
      301 tgcaagacct gggcgtattg ccagggaccg tgatcgaagc cgagttcgcc agccccggcg
      361 gggatccggt ggcctatcga atccgggggg cgacgatcgc cctgcgcaag gagcaggccg
      421 cccacattct ggttgactcc ctggaggaat ccctgtgcgc ttgagccact ccgcctgcgc
      481 ggactgtcct gtgcatcagg ccgccgaccc gtcgacgctt ggcgtagaca ggcagcgctg
      541 ggactacgtg gtggcgctgg ggggtaaccc caacacgggc aagagcacgg tattcaatgc
      601 cctgaccggc ctgcgccagc acacgggcaa ctggccgggg aaaaccgtcg cccgggccga
      661 gggtggttac gagtatgcag gacttcattt caagctggtt gatctaccgg gcacctattc
      721 gctcctggcc accaccgtgg acgagcagat cgcccgcgac tttatcctct ccggccagcc
      781 ggacgtgacc gtcatcgtag tggatgcaac ccgcctggag cgcaacctca acctggtcca
      841 gcaggtactg gagatcaccg accgggtggt ggtctgcctc aacctgatgg acgaggctcg
      901 ccggcatggc ctggccgtcg atgaccgaca gctgacccgt gatctcggcg taccggtcgt
      961 tccgaccgtg gcccggcggg gaaggggact ggatcgattg atgtcggcca tcgcggaggt
     1021 cgccggtggg aagttggtcg gtcggacgcc gcggatcgcc agcccagagt cccaagcgac
     1081 gagggaggca gctttgtcgt cggtttcaaa gaccgatggc aatcgccacg ggcagagcgt
     1141 cgaagcgacc tatgccgaag cggcccgcct tgccggaagg gccgtccgcc aggcaaacac
     1201 tcagccgcgg ttcgatctcg accgggccat cgatcgcatc gtcaccagcc ggatctgggg
     1261 cttcccgatc atgctgaccc tgctggctgg cgtgttctgg atcaccatca ccggggcgaa
     1321 cgtcccgtcc cggctgctcg gcacgttcct gctggacact gtccatccct ggctgcggga
     1381 agccgccgcg gatctcgggg tggccgcctg gctgcgggga gtgctgatcg acgggatgta
     1441 cctggccacc gcctgggtcg tcagcgtcat gctgccgccg atggcgatct tcttcccgct
     1501 cttcaccctg ctcgaggact tcggctacct gccgcggatc gccttcaatc tggacaacct
     1561 cttcaagcga gcgggggccc acggcaaaca ggcgctgacc atggccatgg gctttggctg
     1621 caacgccgcc ggagtgattt cggctcggat catcgacagc ccccgcgagc ggctgatcgc
     1681 gatcctgacc aacaacttcg ccctgtgcaa cggccgctgg ccgacccaaa tcctgatcgc
     1741 caccatcttc cttgggacat tggtgccggc ccggctggcc ggggcggtct cggcgctggc
     1801 ggtggttggc gtggcaatcc tgggagtggg actgacgttc gccgtctcgt ggttgctttc
     1861 ccgcaccatg ctgcgcggcg aagcgtcggc cttcagtctg gagttgccgc cctaccggcc
     1921 gccgcaggtt ttgaagaccc tgtatcgctc cgtgatcgat cggacagtct acgtactctg
     1981 gcgggcggtc gtctttgcca tgccggcggg ggcggtgatc tggatcgtcg ccaacctgca
     2041 acttggcgga atcagcctgg ccgagcggct ggtcgaccta ctcaatcccg ttgggctgct
     2101 gttgggactg aacggcgtga tcctgctggc cttcatggcg gcgatcccgg ccaacgagat
     2161 cgtggtcccg acgatcctga tgctcaccgt gctggccggg gggttgagcg gcgtcggggt
     2221 gggcgcgggt gtgatgttcc aattggagtc tggcgccggg acgctcgcca ttctgcgggc
     2281 cggcggttgg acgctgctca ccggcgtcaa cgtgatgctg ttcagcctgc tccacaaccc
     2341 ctgtagcaca acgatcgcta ccatctataa ggaaaccggc agcaagcgct gggcgctgat
     2401 atccgccgtg ctccccttgg ggattggtct gctgcttacg ggtgtggtgg ctcagctctg
     2461 gcggctggtc gctgcgtgag ctaccagttg aatcttcgag ggggtccggc cagctcacgg
     2521 accgggcgtc tggcgtagtc ctccgtccag cggatcggca ccagggcacc gtgccggagt
     2581 tcgagcccgg tcaagatccc gggcacgact ccgctcccgg tattgaaata ggggatttcc
     2641 ccgatcttgg ggaatgccgg gcggtgggta tggccgcaga tgacgatctc ctcgtaggtc
     2701 ttgatccagt cggtgatacg ctgctcgagt ttccccatct ggttcgcccg gccaggc
//
LOCUS       DATDPQ010000046         4075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3679462, whole genome shotgun sequence.
ACCESSION   DATDPQ010000046 DATDPQ010000000
VERSION     DATDPQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679462"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VI520_01050"
     CDS             complement(<1..1191)
                     /locus_tag="VI520_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36500.1"
                     /translation="MSETLDADVGLMHVAGGQSLQAAPPGTLALGAPRRAARGRVHDF
                     LFLNLKLEGPPGSEGGQLDRLAQETARLFFGTPGSVTAALRQAINQLNQELLDRAVPA
                     RLVAAALRNQNLYLAQCGPSQALLIRSGGVSRLRAEDGTSRSLGTTLAPLIQFRHFEL
                     SPEDLIVLTTNESPLWSDTSLAELSGIDHSLALDRLVASAAQDFTGLSIHIPTVVGAP
                     TAARKGLGAATPAELRPGVRVRSSKARTSPVASRQPAQAVLSAAPVHPARTRAAGIPS
                     WLRAGESEAIRAPAVVTAHPSSPAAGWQAGMRRFRLSLRTGFRSLIESLRRVALGLEV
                     PPLPGMFSSNLLAITAAAVPVLVVGVSSVVYFSRGRLEQFDSYLAQAGGAAIAAQLAE
                     DPQAA"
     gene            complement(1294..2229)
                     /locus_tag="VI520_01055"
     CDS             complement(1294..2229)
                     /locus_tag="VI520_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLF36501.1"
                     /translation="MNPSQESHLTVAALSHPGEAREINEDRYSVSHYLRESDGRPALL
                     AIVADGIGGHQAGEVAAQITIDTVRTSIAAVDGKEPLAELGEAVVSAGRLVMESSALD
                     EALRGMGSTVAVAWIIDHRLYTVSVGDSRIYLLREGRLKQISIDHTWVQEAVLHGILT
                     PEEARVHPNAHVLHRHLGGDRDPVPDFRLRLSDSESDAQSESNQGLQLMAGDQLLLCS
                     DGLTDLVEPAEIQLALAKHSPPEAVSALVALARDRGGHDNITLIAVSVPDPSHSPRAK
                     GCLGRVLRSSLAGVALAALVLLGIAVGYWFDLWPW"
     gene            complement(2226..>4075)
                     /locus_tag="VI520_01060"
     CDS             complement(2226..>4075)
                     /locus_tag="VI520_01060"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF36502.1"
                     /translation="VGIAPCYNRRMALRVVYRLPVLLAAAALLPTQQIQARLNVSQPD
                     VQAFPNISLHAALTDGSGRPIPDLPTDAFQVLEDGEPVEGLAMEKVLVGTRQVFVINT
                     SAGLGVRDSHGRTRFEFARDELIRYWRDPQASLTGVDDLSLLTAEGLLIQHSDSAAEL
                     SALLDTIEPSFEGGISGFDLVLLALDFSSDPAELGLLPGSLIFITPVIETPRDLPLAN
                     AIARAAESGTAIFPVLFSTSETVEPPEVEPLRQLAEATGGRFIYFDSEVGLGELADWF
                     QAQRYQYRLSFDSHTSASGTHSLQVQVAADGLEARSAPISYAIDVQPPEVAFIQTPSE
                     VLRQTEDPDLPIESILPVSLPLEILITFPDGHPRPIQQSRLLVDGELVQTRNAPPYEQ
                     LVWNLASYRQSGSHTVQAVLVDSLGLQTQSIELAVQITIETPPRGLSAFRPAFDSLVA
                     VAALIVAAMVMVGGLTTLVRRSPQSLSSSRVRQSSIKRARLQQSSPSEALLIPVRPAG
                     EPVPLTGVDVVLGRDASLAAIVVADPSVERMHARLIRQADGDYLLRDQGSVAGTWVNY
                     DLIPSEGRRLRNGDLVQLGRVEFRFQLPGKHPPRPILVYPDPQDRSTEA"
BASE COUNT          785 a         1252 c         1356 g          682 t
ORIGIN      
        1 ggccgcctgg ggatcttctg caagctgggc ggcgatcgcg gctcccccag cctgggccag
       61 gtagctatcg aactgctcga gtctgccgcg gctgaaatac accacactcg ataccccgac
      121 aaccaggacg ggaaccgcgg ccgcggtgat ggccagcaga ttggaggaga acatgccggg
      181 cagcggcggg acttccaatc caagcgccac tcggcgcagg ctttcaatca ggctccgaaa
      241 gccggtccga aggctcagcc ggaaccgtcg catccccgcc tgccagcctg ccgccgggct
      301 gctcgggtga gccgtgacga ccgctggtgc cctgatcgct tcgctctcac ctgccctgag
      361 ccacgatggt atgcctgcgg cccgggtgcg ggcaggatgc acgggcgcag ccgagaggac
      421 ggcctgcgca ggctgccggc ttgcgaccgg cgaggtccgg gccttggacg aacgaacccg
      481 cactcccggt cggagctccg ccggggtcgc agcgcccagg cccttgcgag cagcggttgg
      541 tgcgccgacg acggtcggaa tgtgaatgga caggccggtg aaatcttgag ccgcggaagc
      601 gaccagccga tcaagcgcca aggaatggtc gatcccgctg agttcggcga gcgaggtgtc
      661 tgaccaaagc ggcgattcat tggtcgtcag gacaatcaga tcttccggac tcaattcaaa
      721 atggcggaat tggatcaatg gggcaagagt ggttcccagc gatcgactcg tgccatcttc
      781 ggctcgcagg cgggagaccc cgccggagcg aatcaagagt gcctggctgg gaccgcattg
      841 cgccaggtag agattctgat tgcgcaaggc tgcggccacc aagcgggccg gcacagctcg
      901 gtctaacagt tcctggttga gctggttgat cgcctggcga agtgcggcgg tgaccgaacc
      961 tggggtgcca aagaacaagc gagccgtttc ctgcgccaat cggtcgagct gcccgccctc
     1021 gctccccggc ggtccttcca gcttgaggtt gagaaagagg aaatcgtgga cccgaccacg
     1081 ggccgcccgt cggggggctc cgagggccaa ggtcccgggt ggggccgcct gaaggctctg
     1141 cccacccgcg acgtgcatta gcccgacatc ggcgtcaaga gtctcagaca cggcggctat
     1201 tttacggatt cggtctggcc acgccaatcg agatgcaagt tttggacctg ggcggcagtg
     1261 cccggtccgg ggggagaggt taggaggtgt ggctcaccaa ggccacaggt cgaaccagta
     1321 tccgaccgcg atcccaagca gcacgagcgc agccagcgct accccggcca gggaggagcg
     1381 caacactcgc cccaggcagc ccttcgcccg cgggctgtga gatggatccg ggacggacac
     1441 cgcgattaag gtgatgttgt cgtgcccacc ccggtctctg gcgagtgcga ccagggcgga
     1501 cacggcctcg gggggggagt gcttggcaag agccagctgg atctcagccg gctccaccag
     1561 gtcggtcagg ccatcactac aaagcagcag ctgatcacct gccatcagtt ggaggccctg
     1621 attggattca gattgtgcgt cggactctga gtcagacaat cgcaagcgga aatcaggaac
     1681 cgggtctctg tctcctccta gatggcgatg gagcacgtgg gcgttgggat gaacccgggc
     1741 ctcctccggg gtcaggattc cgtgaagaac ggcctcctgg acccaggtgt ggtcgatgct
     1801 gatttgtttg agccgacctt cgcgcagcag atagattcga ctgtcgccaa ccgacacggt
     1861 ataaagtcga tgatcgatta tccaagcgac cgcgaccgtc gatcccattc cccgcagcgc
     1921 ctcgtcgagc gctgacgatt ccatcaccaa tcggccggcc gagacgaccg cctcgcccag
     1981 ctcagccagg ggttctttgc cgtccactgc ggcgatcgaa gtacgcacgg tgtcgatcgt
     2041 gatctgagcg gcgacctcgc ctgcctggtg cccgccgatc ccatccgcca caatggccag
     2101 cagtgcgggt cggccgtcgg actcgcgcag gtagtgggaa accgaatacc ggtcttcgtt
     2161 gatttcgcgg gcctcgccgg gatgggaaag cgctgcgacc gtcaaatgtg attcttggga
     2221 tgggttcatg cttcggtcga acgatcttgc gggtcgggat aaacgaggat tggccggggt
     2281 gggtgcttgc ccgggagttg gaagcgaaac tccacccgcc ccaactgcac cagatcgccg
     2341 ttgcgcagcc ggcgtccctc ggaagggatc aaatcgtaat tgacccatgt cccggccacg
     2401 ctgccctggt cgcggagcag atagtcgccg tcggcctgcc ggatcagtcg agcgtgcatg
     2461 cgctccacag agggatccgc aaccacaatc gcggcgagcg aggcgtctcg gccaagcacg
     2521 acatctacac cggtaagcgg gaccggctcg cccgcgggtc ggactggaat gagtagcgcc
     2581 tccgaggggg aggactgctg caggcgggcg cgtttgatcg agctctggcg aacccgggaa
     2641 gagcttagtg attgggggga ccggcgcacc agggtcgtca atccgccaac catgaccatc
     2701 gcggcaacaa tcagggccgc cactgcaacc aacgaatcga acgcgggtcg gaaggccgag
     2761 agaccccgcg gtggggtctc gattgtgatt tggaccgcca actcaatgct ctgggtctgc
     2821 aaacccagcg aatcgaccag gactgcctgc accgtatggg acccgctctg gcgatacgaa
     2881 gcgaggttcc acaccaactg ctcataagga ggcgcgtttc gggtctgcac caattcaccg
     2941 tcgaccaaca accggctctg ttgaatcggt cgcgggtgcc catccggaaa ggtgatcagg
     3001 atctccaacg ggagcgacac cggcaggatg gactcgatcg gcagatcggg atcctcggtc
     3061 tgccgcagca cttcgcttgg agtttggatg aaggcgacct cgggcggctg gacgtcgatc
     3121 gcgtagctga tcggcgccga acgtgcctct aggccgtcgg cagccacttg gacctgcaaa
     3181 ctatgggtgc cggaggcgct ggtatgagaa tcgaagctca atcggtattg gtaacgctga
     3241 gcttggaacc aatcggccaa ctcgcccagc ccgacctccg agtcgaaata gatgaaccgc
     3301 cccccggtgg cttcggccag ttggcggagc ggttctacct caggcggttc gacggtctcc
     3361 gaggtcgaga acaagaccgg gaagatggcc gttccggact cggcggcccg tgcgatcgcg
     3421 tttgccagcg gaagatcgcg gggagtctcg atgactgggg taatgaagat cagcgatccc
     3481 ggaagcaagc ccagctcggc gggatcggaa gagaagtcaa gtgccagcag gaccagatcg
     3541 aatcccgaga tcccaccttc aaagctcggt tcaatcgtat ccagcagggc ggaaagttcg
     3601 gcggcggaat cgctgtgctg gatgagtagg ccctcagcgg tcagcaggct caggtcgtcg
     3661 actccagtga ggctcgcttg agggtcgcgc cagtagcgga tcagctcgtc tcgggcgaac
     3721 tcgaaacggg tgcgaccgtg cgagtcccgc acgccgagcc ccgcagaggt gttgatcaca
     3781 aacacctgcc gggtgccgac cagcaccttc tccatggcca gtccctcaac cggttccccg
     3841 tcttccagca cttgaaaggc gtcagtcgga agatctggga tcggccgccc ggacccatcg
     3901 gtgagggcag cgtgcagact gatgttcggg aaggcctgga catcgggttg ggatacgttc
     3961 agtcgggctt ggatctgctg ggtgggtaac agggcggccg ccgcgagcag caccggcagc
     4021 cgatagacca cccggagggc catccgccga ttgtagcatg gggctattcc cactc
//
LOCUS       DATDPQ010000047         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_18157, whole genome shotgun sequence.
ACCESSION   DATDPQ010000047 DATDPQ010000000
VERSION     DATDPQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18157"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1341)
                     /locus_tag="VI520_01065"
     CDS             complement(58..1341)
                     /locus_tag="VI520_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLF36503.1"
                     /translation="MTENLLTRRDFLRLAGISAAPLALPGWTPRLAFAPPGSPPSGDL
                     LVCVFLRGGMDGLNAVIPHFESAYYDARSTLAIAEPGSGDDTAAVELDDRFSLHPNLR
                     PLLGPFQAGELAIVHACGSPDPTHSHFDAMDFMERGTPGEKQIATGWIARHLLTAPWE
                     NDSPFRAVGIGVGLQASLRGPVPAAALRSVADFHLPGDPQGVATLQNTLSSLYQLPAP
                     EESLLAALSSQAGTTLGAIQLLAGLDPDSYRPEGGAAYPDDDYGRGLAQIALLAKAEL
                     GLEIACLDVGGWDTHVQQGAQDGQMPQLMTSLAQGLAAFHQDMGLRMKDITVVTMSEF
                     GRRVAENGGGGTDHGHGGVMFVMGGGILGGKVYGDWPGLAPESLYGPGDLAITTDFRE
                     VLGEIVQKRLGNSRIDEIFPGYRFAGFLSLATERS"
     gene            complement(1354..>2007)
                     /locus_tag="VI520_01070"
     CDS             complement(1354..>2007)
                     /locus_tag="VI520_01070"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HLF36504.1"
                     /translation="QVLDILAEHPSTARFIATKLCARFVADVPPGSLVARVADSYLQT
                     GGDIPAMLRTILYSEEFAASAGLKLKRPLDFAVSAIRVTGADGRLERLFRLYLEALGQ
                     IPFGWPTPDGYPDYAAAWTSTSQSLHRWNLALSLTSGLLPGLSTQLPNLSNNASELPD
                     MIDELSQALLGTLLPEEARSILIEFAGSLQSTDLPDGIELPDLLAGLVLCTPHFQIR"
BASE COUNT          360 a          633 c          691 g          323 t
ORIGIN      
        1 aaccgcaagt gtttcctaag aagtgagcga atcacatcgc aggaaggtgc ggatgcgtca
       61 gctgcgctcg gtcgccaggc tgaggaaccc ggcgaatcgg taaccgggaa agatttcatc
      121 gatccgactg ttcccgagcc gcttctggac gatctcgccg agcacttccc gaaagtcggt
      181 cgtgatcgcc agatcgccgg ggccgtatag actctcaggg gccagccccg gccagtctcc
      241 atagacctta ccgcccagga ttcctccgcc catcacaaac atcacgccgc cgtgcccgtg
      301 atcggtgcct ccgccgccgt tttcggcgac ccgccggcca aactcggaca ttgtcaccac
      361 tgtgatgtcc ttcatcctga ggcccatgtc ttggtggaag gccgccagtc cctgggcgag
      421 cgaggtcatc agctgcggca tttggccgtc ttgggcacct tgttgaacgt gagtatccca
      481 accgccgacg tcgaggcagg cgatttccag cccgagctcg gccttcgcca gcagcgcgat
      541 ctgggccagc cctcgaccat agtcatcgtc gggataggcc gccccgcctt ccggccgata
      601 gctgtccggg tcgagcccgg ccagcagctg gatcgccccg agggtggtgc cggcctggct
      661 ggagagcgcc gccaacaggc tctcctccgg cgcgggcagt tgatacaggg aactcaacgt
      721 gttctgcaga gtcgcgacgc cttgcggatc gcccggcagg tggaaatcgg ccacggatcg
      781 aagggccgcc gccggcaccg gcccgcgcaa ggaggcctgc agccccaccc cgatgcccac
      841 cgcccggaag ggggagtcgt tctcccaagg tgcggtcagc aggtggcggg cgatccagcc
      901 ggtcgcgatc tgcttctccc ccggggtacc gcgctccatg aagtccatcg cgtcgaagtg
      961 cgagtgagtg gggtcgggcg agccgcaggc atgtacgatg gccagttctc cggcctggaa
     1021 cggaccgagc agcgggcgca ggttcgggtg caggctgaag cggtcatcga gctcaaccgc
     1081 cgcggtatcg tcgccggagc caggctccgc gatggccaga gtggagcggg catcgtaata
     1141 ggcactctcg aagtgtggaa tcaccgcgtt caatccatcc atgccgccgc gcaagaacac
     1201 acacaccagc aggtcgccgg acggcgggct gcctggtggg gcgaacgcca gtcgaggggt
     1261 ccagcccggg agcgccagtg gggcggccga gatgccggcc agtcggagaa agtcgcgtcg
     1321 ggtgaggagg ttttcagtca tggattcacc tggtcaacgg atttggaagt ggggtgtgca
     1381 gagcacgagg cccgccaaga ggtcgggaag ttcgatcccg tccggcagat cggtgctctg
     1441 cagcgagccg gcaaactcga tcaggataga tcgggcctcc tcgggcagga gcgtgccgag
     1501 cagggcctgg cttagctcgt cgatcatgtc cggtagctcc gaagcattgt tggataggtt
     1561 tggaagctgg gtgctgagcc ccggcaacag gccagaggtc aggctgaggg ccaggttcca
     1621 gcgatgcagc gactggctgg tgctggtcca ggctgcggcg tagtcgggat acccatcggg
     1681 cgtcggccac ccgaagggaa tctgtcccag cgcctccaag taaaggcgga agagccgctc
     1741 cagccggcca tcggcgccgg tcacccggat ggccgagacc gcgaagtcga gcggtcgctt
     1801 gagcttaagc ccggccgagg ccgcgaactc ttcagagtac aggatcgtcc gcagcatggc
     1861 cgggatatcg ccgccggtct gcaggtagct gtcggccact cgcgcaacca gtgagccggg
     1921 cggaacatcg gcgacaaacc gcgcgcagag cttggtggcg atgaagcggg cggtggaggg
     1981 gtgttcggcc aggatgtcca acacctg
//
LOCUS       DATDPQ010000048         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_799710, whole genome shotgun sequence.
ACCESSION   DATDPQ010000048 DATDPQ010000000
VERSION     DATDPQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799710"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1147)
                     /locus_tag="VI520_01075"
     CDS             complement(5..1147)
                     /locus_tag="VI520_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36505.1"
                     /translation="MPGLRSIRRRLGRKLLLSYLIIIVVGVVVLASAAEFTIPTSFDR
                     HMLGMQPMMGGAEMGMGRDADLFASFRAAVSESLLLAAVAATVAAVVVSLLVTRRVVA
                     PVRQMMVASRRIAEGNYAERVQVRGAPEPDEMDELDQLAVTFNQMADKLERTEAMRRQ
                     LIGDVAHELRTPLSTIKGSVEGLMDGVLEPTEANLHQIYREADRLQRLVGDLQELSRV
                     EAGAFELHRKAVEVEELVSSAAERLAGQFDEKGVRLEIDLAADLPKVLADPDRIGQVM
                     LNLLGNALQYTTSGRTVEVQARRRDRFVEIEIRDEGMGIPAEHLPHVFTRFYRVDRSR
                     SRAGGGSGIGLTIAKHLVEAHGGQIWASSPGSGKGSSFTFTLPLAG"
     gene            complement(1150..1830)
                     /locus_tag="VI520_01080"
     CDS             complement(1150..1830)
                     /locus_tag="VI520_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36506.1"
                     /translation="MAEAKILVVDDEQSILDLVTAYLKPEGYQVYTAMDGPAALKAAR
                     AFRPDLIVLDIMLPGMDGVEVLSQLRRESPVYVILLTARAEETDKIVGLSVGADDYLT
                     KPFSPRELVARIKAALRRLRQGHAVPEESTLSFPHIRIDAGSRKVWVDDQPVELTTIE
                     FDLLKTLAEYPGRVLAREQLLEKVWGYDYYGEIRVVDVHVGHIRRKLGADRFISTVRG
                     VGYRFEDA"
     gene            complement(1845..>2271)
                     /locus_tag="VI520_01085"
     CDS             complement(1845..>2271)
                     /locus_tag="VI520_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36507.1"
                     /translation="ASTGIGAMEVLVDPVTRAVYPEHGPNMMWNLKYSPMAGSGMMGG
                     MMGWYGGQSPASGLGRLDPSAEMSVTAAEAVEAAQDYLDRYLPGTVADEQVDPFYGYY
                     TLHILREGGTVGMLSVNGFSAQVFLHSWHGDFVEMTEEE"
BASE COUNT          412 a          781 c          670 g          408 t
ORIGIN      
        1 aggctcagcc ggcgagcggt agcgtgaacg tgaagctgct tcctttgccg gatcccgggc
       61 tgctggccca gatctgccca ccatgagctt ccaccaggtg cttggcgatg gtcaggccga
      121 tcccgcttcc gcctcctgcc cgcgaacggg atcggtccac ccgatagaag cgggtgaaga
      181 catgcggcag atgttcagcg ggaatcccca tcccttcgtc acggatctcg atctccacga
      241 accgatctct ccgacgggcc tgcacttcga ccgttcggcc agaggtcgtg tactgcaaag
      301 cgttcccaag aaggttcagc atcacctggc cgatgcggtc cgggtccgcc agtactttgg
      361 gcaggtcggc tgccaggtca atctccagcc ggactccttt ctcatcgaac tgcccagcga
      421 gccgctccgc ggcgctggac accagctctt cgacctccac ggcttttcgg tgcaactcaa
      481 aggcaccggc ttccacccga ctgagctcct gcagatcgcc gaccagccgc tgcaaccgat
      541 ccgcttcccg atagatctga tgcaagttgg cctcggtcgg ctcgagcacg ccgtccatca
      601 ggccctcgac cgatcctttg atggtgctca gcggggtccg cagctcgtgc gctacatccc
      661 cgataagctg cctccgcatc gcctcggtcc gctccagctt gtcggccatc tgattgaagg
      721 tcacggctag ctggtccagc tcgtccatct catcaggctc cggagcaccc ctaacctgca
      781 cccgctcagc atagtttcct tccgcgatac gccggctggc gaccatcatc tggcggaccg
      841 gagcgaccac ccgccgggtg accagcaggc tgacgacgac cgcagccacc gtcgcggcca
      901 cggccgccag cagaagcgac tcgctgaccg cggcgcggaa actggcaaac agatccgcat
      961 cgcggcccat ccccatttcg gcaccgccca tcatgggctg catcccgagc atgtggcgat
     1021 cgaaagaggt cgggatcgta aactcggccg cagaggccag caccaccacg cccacgacga
     1081 tgatgatcaa gtaggagagc aagagctttc ggcccagccg gcggcggatc gaacgcagcc
     1141 caggcatctc taggcgtcct cgaatcgata gccgacgccg cgcacggtcg aaatgaaccg
     1201 gtcggcgccc aacttgcgcc ggatatggcc gacgtgcacg tcgacgaccc ggatctcgcc
     1261 gtagtagtcg tatccccaca ccttctcgag aagctgctcc cgggcgagca cccggcccgg
     1321 gtattcggcg agggtcttga gcaggtcgaa ttcgatggtg gtcagctcga cgggctgatc
     1381 gtccacccag accttgcggc tgccggcgtc aatccggata tgtggaaagg agagcgtgct
     1441 ttcctcggga acggcgtgcc cctggcggag ccggcgcagc gcggccttga tccgtgcgac
     1501 gagctcgcga gggctgaagg gcttggtcag atagtcgtcg gccccgaccg agaggccaac
     1561 gatcttgtca gtttcttccg ccctggcggt cagcagaatg acatagaccg gcgactcgcg
     1621 gcggagctgt gagagcactt cgaccccgtc catccccgga agcatgatgt ccagcacgat
     1681 caggtcgggt ctgaaggccc gggctgcctt gagcgccgcg ggcccatcca tcgccgtgta
     1741 gacctggtag ccctccggct tcaggtaggc ggtcaccagg tccagaatgc tctgttcgtc
     1801 gtcgacaacc aggatcttgg cctcggccaa gggaaacctc cagcctactc ttcttcggtc
     1861 atttcgacga agtcgccgtg ccagctgtgc aggaatacct gggctgaaaa cccattcacg
     1921 ctcagcattc cgaccgtgcc accctcccgc aggatgtgca gagtatagta gccatagaag
     1981 ggatcgactt gctcgtcggc cacagtgccc ggcaggtaac gatccaggta gtcctgggcc
     2041 gcctcgaccg cctctgccgc ggtcacgctc atttcagcgg acggatcgag tcgtccgaga
     2101 ccgctagccg gtgattggcc tccataccaa cccatcatgc cccccatcat gcccgagccg
     2161 gccatggggc tgtatttgag attccacatc atgttggggc cgtgctcagg gtaaaccgcc
     2221 cgggtcaccg gatccaccag cacttccatg gcgccgattc cggtgctggc c
//
LOCUS       DATDPQ010000049         4883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1846128, whole genome shotgun sequence.
ACCESSION   DATDPQ010000049 DATDPQ010000000
VERSION     DATDPQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846128"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VI520_01090"
     CDS             complement(<1..1028)
                     /locus_tag="VI520_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36508.1"
                     /translation="MKIDRGLVRDQQAADSLVLTLRDWRAVLAERSELVVFITVAAVF
                     GFFALSAEHFLSPFALSNILTFASINGIVVIAVALLMISGEFDLSVGSIFAVASYVFA
                     LSLIDGVAPILALLLALIASGLLGLINGWIVTKSGIPSFITTLGTMLAFRGIVRAIGG
                     GVFASYGGPRLAIFDVLNGPIGPINRLSQPAANFRISVLWFVLLAAVMGFVLLRTRYG
                     NWVFATGGNPRAANAQGVPINRVKVINFMLTGLLAGAAGVIQFGHRTSVDPLRGDGIE
                     LIAVAACVIGGVRLSGGFGTIVGPCIGVLLLQMLEQGLVLMRVPVQVFQAVAGFFLIL
                     AVVMNISLG"
     gene            complement(1089..1976)
                     /gene="iolB"
                     /locus_tag="VI520_01095"
     CDS             complement(1089..1976)
                     /gene="iolB"
                     /locus_tag="VI520_01095"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HLF36509.1"
                     /translation="MTTSKLLVVPQPEVAEYQRVTPESAGWQLLHFAARLLQPKESWE
                     FDTGDCEYGVVVLGGVCRVDTSRGEWERVGRRPNVFAGMPHALYLPRRTQVRLLASEG
                     PLDLAYGWCPTDQDHPARFVPPHEVRIEIRGGGNATRQIHDLIPPGLDCHRLVAVEVY
                     TPSGNWSSYPPHKHDQHRQDNHGNLLEADLEEIYFYKLDRPEGYAIQRVYTSDGRLDE
                     LILARDNDVVLVPEGYHPVVSAHGYTTYYLNFLAGSAQSLANSDDPAYAWIKGVWQEQ
                     DPRLPLVSLEMEAGGDSSG"
     gene            complement(2114..3235)
                     /locus_tag="VI520_01100"
     CDS             complement(2114..3235)
                     /locus_tag="VI520_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HLF36510.1"
                     /translation="MAGRSDRVGVAIIGVGNCASSLVQGVEYYKNASEDQWIPGLMHA
                     NLGGYHVRDIHFTAAFDVVSTKVGRDLSDAIWAHPNNTIKFAEVPYLNVPVHRGMTHD
                     GLGKYLKMVVEKAPGPTDDMVRILRETGTDVVVNFLPVGSEMATKWYVEQVLEAGCGL
                     VNCIPVFIASQEYWVGRFTEHGVPIIGDDIKSQVGATITHRVLTNLFRDRGVRIDRTY
                     QLNFGGNMDFYNMLERERLESKKISKTQAVTSQLPYELEPGNVHVGPSDYVEWLTDRK
                     WCHIRMEGRAFGEVPISLELKLEVWDSPNSAGVVIDAVRCAKLAMENGISGSLDGPSA
                     YFMKSPPVQYNDDIARRMTEEFVRQTSSPVVPRTAAQEA"
     gene            complement(3272..4456)
                     /locus_tag="VI520_01105"
     CDS             complement(3272..4456)
                     /locus_tag="VI520_01105"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HLF36511.1"
                     /translation="MPMRIVVLGGAGIVGRAIAADLARQVDQVVVADLDPESARAAAA
                     DAGATAQSGSVDVRDHPALVKVLQGAAACVNSVNYYFNLDVMQACLEAGVPYLDLGGL
                     FHMTRRQLELDSDFRARGLTAVLGIGSCPGVANVQAGWLAGTLDQVDSVRIFNGSTLD
                     RGDSLSAPYAIQTILDEISQPAMVFREGRFQERSPLSEEEHHHFPEPIGWAKTHLSLH
                     SEVATIPLSFAEKGIRECAFKITFFGYSEAALRKLQFLAEIGLASTEVVELNGVSIRP
                     RDLLVKLLGRLQAGEAEPANEGYKAVVTEIKGKIGARPVVLRAETVGGPLAGQTAGAG
                     KRLIAGPAAIVGRALAEGRLRRPGVWAPEQAIDPEPFFAELAQRGFTTRLSKQETVAA
                     PS"
     gene            complement(4457..>4883)
                     /locus_tag="VI520_01110"
     CDS             complement(4457..>4883)
                     /locus_tag="VI520_01110"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HLF36512.1"
                     /translation="MELMSALLHNGILGLDTIQLDGRELPALEALRALLWQNPVSKQN
                     PVWAYGLVVEVTGERAGRRELHRYRNHHPPQQEWGGEAAYYKNVGLPLSIGAQMIARG
                     EVTGHGVLPPERAFPVAPFFQALAERGITIEETVEELAA"
BASE COUNT          957 a         1584 c         1467 g          875 t
ORIGIN      
        1 cccagcgaga tgttcattac aacggccagg atcaggaaaa atcctgccac ggcttggaaa
       61 acttgaactg gcaccctcat caatacgagc ccctgctcca gcatctgcaa cagcaggacc
      121 ccgatacaag gtcccacgat cgttccgaag ccaccactga gacgcacgcc cccgattacg
      181 caagccgcca cggcaatcag ctcgattcca tcgccacgca ggggatccac gctggttctg
      241 tgcccaaatt ggatgacccc ggccgcgccg gccagcaacc cggtgagcat gaaattgatc
      301 accttgaccc ggttgatcgg cacaccctgg gcattggctg cccgggggtt ccctccggtc
      361 gcaaacaccc agtttccata gcgcgtgcgc agcagaacga accccatgac ggccgcgagc
      421 aacacgaacc acagaactga gatccgaaag ttggccgcgg gttgggacag ccggttgatc
      481 ggtccgattg ggccgttgag cacgtcgaag atggccagcc gagggccgcc ataggaagcg
      541 aacactccgc cgccgatggc ccgcacaata ccgcggaacg cgagcatcgt gccaagcgtc
      601 gtaatgaagg aaggaatgcc ggacttggtg acgatccatc cgttgatcag gccgagcaag
      661 ccgctagcga tcaacgccag cagcagtgcg aggatcgggg cgaccccgtc gatcagcgat
      721 agtgcgaata catagcttgc cacggcaaag atcgagccga cggataaatc gaattcgccc
      781 gagatcatga gcaacgcaac cgcgatcacg acgatgccgt tgatgctggc gaaagtcagg
      841 atgttcgaga gggcaaaggg ggagagaaag tgctctgctg agagcgcaaa aaagccgaaa
      901 acggccgcca ccgtgatgaa gaccaccagt tcggaccgct cggccagcac agcccgccag
      961 tcgcgcagtg tgagcaccag cgaatcagcc gcttgttgat cacggaccag cccgcggtca
     1021 atcttcatcg atgcactgcc ggacatccgg ggttggctag cttcacagca agacatgggt
     1081 tgagctggtc acccactcga atccccacca gcttccatct caagcgacac gagtggtagc
     1141 cgaggatcct gctcctgcca cacgcccttg atccatgcgt aggccgggtc gtccgaattt
     1201 gcgagcgact gggcactgcc ggccaggaag ttcaagtaat aggtcgtgta cccgtgggcg
     1261 gacacgaccg ggtggtaacc ctccgggacc agcacgacgt cgttgtcccg ggccaagatg
     1321 agttcgtcca gtcggccgtc cgaagtgtaa acccgctgga tggcataccc ttccggccgg
     1381 tccagcttgt agaagtagat ctcctccagg tcggcctcca ggaggttgcc gtggttatcc
     1441 tgccggtgtt ggtcatgttt gtgaggcgga taagagctcc agttgccgga gggcgtgtac
     1501 acctcgactg ccactaggcg gtgacaatca agaccgggcg ggatcaggtc gtggatctgg
     1561 cgggtggcgt ttcccccgcc ccggatttcg atccggactt cgtggggggg aacgaagcgc
     1621 gccgggtggt cctggtcagt ggggcaccag ccatatgcca ggtcgagcgg cccttccgac
     1681 gcgagcagcc gcacctgggt ccggcggggg aggtagagcg catgcggcat tcccgcgaac
     1741 acgtttggcc tgcgcccgac ccgctcccat tcgccacgcg aagtatccac tcggcagacg
     1801 cctccgagca cgacaacccc atactcacaa tcgccggtgt cgaactccca gctctccttc
     1861 ggttgcagga ggcgggcagc aaaatggagc agttgccagc cggcagattc aggggtaacc
     1921 cgctggtatt cggccacctc aggctgtgga acaacgagca gcttagaggt ggtcatcccc
     1981 ctccgaggtg ggcgcactga ttcgctcgaa gagttgccga tcgactagtt gggatcaatc
     2041 gcagggtcaa ggggcacgga gctggtaggg cttggggcga gttcggccgc gggccagagc
     2101 catcggccgt gggtcaggct tcttgggcgg ccgtccgagg aactacgggc gaggaagtct
     2161 gacggacgaa ttcttcggtc atccggcggg cgatgtcgtc gttgtactgc accggcgggc
     2221 tcttcatgaa gtaggcggat ggcccgtcca gcgaacccga gatgccattc tccatggcca
     2281 gcttggcgca gcgcacggcg tcgatcacca ccccggcgga gttcggcgaa tcccagacct
     2341 ccaacttcaa ctcgaggctg atcggtactt caccgaacgc ccgcccttcc attcggatgt
     2401 ggcaccactt gcggtcggtc agccactcga cgtagtcgct aggccccacg tggacattcc
     2461 ccggctcgag ttcgtagggc aattggctgg tgaccgcctg ggtcttcgag atcttcttgg
     2521 actccaatcg ctcccgttcc agcatgttgt agaagtccat gttgccgccg aagttcaatt
     2581 ggtaggtccg gtcaatccgc accccccgat cccggaacag gttagtcagc acccgatggg
     2641 tgatggttgc ccccacctgg ctcttgatgt cgtctccgat gatcggaacc ccatgctccg
     2701 tgaagcgacc gacccagtac tcctggctgg cgatgaacac cggaatgcag ttcaccaacc
     2761 cacagcccgc ctccagcacc tgttctacat accacttggt ggccatctcg gatccgaccg
     2821 gaaggaaatt gacaaccaca tcggtgccgg tctcccgcag gatgcggacc atgtcatcgg
     2881 tcggaccggg tgccttctct accaccatct tgagatactt gcccaagcca tcatgggtca
     2941 tcccgcggtg gaccgggaca ttcagatagg gcacttcggc aaacttgatc gtgttgttgg
     3001 ggtgggccca gatcgcgtcg ctcaagtccc gcccaacctt ggtggagacg acatcgaagg
     3061 ccgcggtgaa gtggatgtcc cgaacgtggt acccgcccag gttggcatgc atcagcccgg
     3121 ggatccactg gtcctcggaa gcgttcttgt agtactcgac cccctgtacc aaggatgagg
     3181 cacagttgcc aaccccgata atcgcgacac ccactcgatc acttcgacct gccatggatt
     3241 tgaacccctc ctcagtttgt cagccggcag ctcacgacgg cgctgccacg gtctcttgtt
     3301 tggaaagccg agtcgtgaag ccgcgctggg ccagctctgc gaagaacggt tctggatcga
     3361 tcgcctgctc gggggcccac acccccggcc gcctcaagcg gccctctgcc agggcccggc
     3421 ctacaatcgc ggccggaccg gctattagcc gcttgccggc cccagcggtt tgacccgcaa
     3481 gggggccgcc cacggtctcg gccctcaaca ccaccgggcg agcaccgatt ttacctttga
     3541 tttcggtcac caccgccttg taaccctcgt tagctggctc cgcctctccc gcctggagcc
     3601 gacccaggag tttcaccagt aaatcccggg gccgaatcga gaccccgttc aattccacca
     3661 cttcggtgct ggccaggcca atctctgcga ggaactgcag cttgcgcaag gcggcctccg
     3721 agtagccaaa gaaagtgatc ttgaaagcgc actcccggat ccctttctcg gcgaagctga
     3781 gcggaatcgt ggccacttcc gagtgcaggc tcagatgggt cttggcccag ccgatcggct
     3841 ctgggaagtg gtggtgctcc tcttcgctga gcggcgaccg ctcctgaaag cgaccctccc
     3901 gaaacaccat cgccggctga ctgatttcat ccaggatggt ctggatcgcg tagggggctg
     3961 aaagcgagtc tccccggtcc agggtggatc cgttgaaaat gcgcaccgaa tcgacttgat
     4021 cgagtgtgcc ggccagccaa ccagcctgga cattggcgac ccccgggcag gaaccgatcc
     4081 ccagtaccgc agttaagccc cgagccctga aatcagagtc cagctcgagc tggcggcggg
     4141 tcatatggaa aaggccccct agatcgagat acggaactcc ggcctccaag caggcttgca
     4201 tgacatccaa gttgaaatag tagttgacgc tgttgacgca ggcggcggcg ccctgcagga
     4261 ccttgaccag cgcaggatgg tcccgtacgt ccacgctgcc cgattgtgct gtggctccgg
     4321 catccgccgc tgccgcccgg gcggactccg ggtccaggtc ggctacgacc acctgatcca
     4381 cctggcgggc caggtctgcc gcaattgccc ggccaacaat tccggctccg cccagtacga
     4441 cgatgcgcat cggcattcag gccgcgagct cctcgaccgt ctcttcgatc gtgatcccgc
     4501 gctccgccag ggcttggaag aagggagcca ccgggaaggc tcgctctgga ggcaacactc
     4561 cgtggcctgt cacctcgccc cgggcaatca tttgcgcccc gatcgagagc ggcaagccca
     4621 cattcttgta gtaggccgcc tctccgcccc actcctgctg cggcggatga tggttgcgat
     4681 agcggtgaag ctcgcgccgg cccgcccgct cgcccgtgac ctcgaccacc aggccatagg
     4741 cccagaccgg gttttgtttc gagaccgggt tttgccagag cagggcccgg agggcctcca
     4801 gggctggcag ctcccgcccg tcaagctgga tggtgtcgag cccgaggatc ccattgtgta
     4861 gcagggcact catgagctcc atc
//
LOCUS       DATDPQ010000050         9384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1064083, whole genome shotgun sequence.
ACCESSION   DATDPQ010000050 DATDPQ010000000
VERSION     DATDPQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064083"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VI520_01115"
     CDS             <1..417
                     /locus_tag="VI520_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HLF36513.1"
                     /translation="SELHTRFLTPRLDARLKQPSLPEAFRTVLAKRGLADVIEIFKPA
                     EVANPHADLAELADLLLEFDHFFQLWRFNHLTMVQSMIGRKSGTGFLGPEYLKETVGL
                     GMQGEDDRLLRTPQIRPRFFEELWEARTRMQVGEQE"
     gene            447..1598
                     /locus_tag="VI520_01120"
     CDS             447..1598
                     /locus_tag="VI520_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLF36514.1"
                     /translation="MNEIDWAAWREEFPGLRRSTYLNTVSLGQLSVRSRAAMNQFMDL
                     WTESGSAAWYAFWLKEQQATREEFARLIGADPQEVAIMPNVSSALGAISSCLDFAQRS
                     QVVTTALDFPTLTYHWLAKEREGLEAVILAAPDGIQVPLSAFERAVGGNTALIATSRV
                     YFTTGFIQDVDALAELAHRNGALLLVDDYQATGQVPIDVRQTPVDVLVTGGLKWLLGG
                     PGIAYLYVRRELIQTLQPTMTGWFSNARQFDFDPHRLEFHEDARRFEAGTPSVASIYA
                     GKAGLEIINEIGAVNVRHRTAELREDLVGRLTDRGFRLRLPPDPDTRSGITIVELEDP
                     MAVTRALAERRIIVDKRPGAVRLSPYFYNTPEENELVVEALTEITRGSG"
     gene            complement(1608..2402)
                     /locus_tag="VI520_01125"
     CDS             complement(1608..2402)
                     /locus_tag="VI520_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLF36515.1"
                     /translation="MAIVYRYAELRPDQLSQRLEEVPVAIVPWGALEWHGPHLPFGLD
                     GLVAESFCQELAVRTGAVLLPTTYLPITALPHPQSISIRTEVVRGAWQDLLHELGKAG
                     FRVVCLVSGHYAQGHEIVLAEVADQLSHRGGPLTLAGAPLALLQDPGLLDHAGEWETS
                     QLLHYRPELVDLTALPPGSLPPAGQVAVLGDDPRKGSPEQGRAIVERALQAWATWMER
                     LLGGQALGALHDLYSARIASYQAYLDRYYQGSWEAAIEAWWKERTG"
     gene            2437..3981
                     /locus_tag="VI520_01130"
     CDS             2437..3981
                     /locus_tag="VI520_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HLF36516.1"
                     /translation="MRINIVGGGPAGLFFALLMKKLDSRHEITLYERDPAGNSYGWGI
                     VLSERSLATLRGADFESYADLIEVAELWKNVEVVHAGERITIGGNEFSGLERIGLLNI
                     LQRRCQALGVKLEFEANVDGLDGVSDCDLLVGADGLKSTVRETDRAQFLPRIEERPNK
                     YIWYGSPRAFSGLTMIFRPHDDGLYIAHAYRFQPKLSTFIIECDPDTWSRGGHSGRTE
                     EQTAAYLAEVFRDDLGGAPLLSNRSLWYNFLLVRNGNWHHRNVVLLGDALHSVHFSIG
                     SGTTLAIEDAIALAGALAQEASVAAGLEAFERLRKPRVDSLQDAAIDSLVWLENVKGD
                     LDLHPLPFAFRLMSRSNRVNYRRLKQQDPGFVARYESWRWENEGPIHREFLDLFQKKA
                     YGHVATLMKDGRPHVTPVYVDFDGRYILINSTKGRQKDLNMERRRKVALEIPDPDQPL
                     RYLGIQGEVVEITEEGAEAHLDKLAQRYHGRERYPASWKFPREVRRIYKIRPDKVIHW
                     DPFGGW"
     gene            4016..4804
                     /locus_tag="VI520_01135"
     CDS             4016..4804
                     /locus_tag="VI520_01135"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HLF36517.1"
                     /translation="MAEHTFEVQDGIGVLTFNRPDVLNALTLELYAEIIELLGKLQFD
                     EQVKVLVLTGAGRAFCSGGDVHKIIGELLAKDMRAHLEFTRMTGTVVRNMRSLDKPII
                     AAVNGVAAGAGAVIALASDLRVVAESAKFAFLFTKVGLAGADMGAAYLLPRVVGMGRA
                     AEILMMGENIDAASAQRIGLANRVVPDGESLPVALEWARKLAAGPSLALATTKRMLEN
                     EWNMGLLAALESEAQAQALMLMGDDHQAFYEAFKQKEPPRFTGR"
     gene            4810..5205
                     /locus_tag="VI520_01140"
     CDS             4810..5205
                     /locus_tag="VI520_01140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLF36518.1"
                     /translation="MTPAINPPELGRPSGFSYAFDARGERTVHFSGHTALDGSGMIVG
                     PGNLAAQFEQTLNNLRATAAAAQVELKDLVKLTIFVTDVEAYKDRSTEIGAVYRRYFG
                     SHFPAMTLVGVTRLWEADAMIEIEAVAVR"
     gene            5213..6406
                     /locus_tag="VI520_01145"
     CDS             5213..6406
                     /locus_tag="VI520_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF36519.1"
                     /translation="MDFNLTEPQIRIQSLVREFAQHEVAPLARTMDEQGRMAPELVSR
                     MAELGLLGGPLPTEYGGGGWDHMSLALAYEELGRADSSLRGFMTVHTSLVSLALLGMG
                     NEAQKASYLPRLAAGETIGCYCLTEPNAGSDAAALECQARLDGDRYLLNGEKIWITNG
                     GMAGLAIVFATLEPAARHRGIAAFLVMTDAPGLRREPMRGKELGHRAADHARFVFTDC
                     RIPAGSLLGKPGEGFQVAMGALDHGRLGVAAGALGVAQACLDACVEFSRNRRQFGKPI
                     GDFGMIQTSLADMAVEVEAARVLVYRAAWLKDQGRPAIRETCMAKLYATEAAARAAGA
                     AVVIHGGRGYSNEYPVERYYRDIKGLQIYEGTSHIQRIVIAREFLGRAAGGPGRVSPG
                     TGKEQ"
     gene            6408..7988
                     /locus_tag="VI520_01150"
     CDS             6408..7988
                     /locus_tag="VI520_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HLF36520.1"
                     /translation="MAVKLAERTVMKAADLPPYYNAVHILEHNLVERAEKVALYSPLR
                     NLTFRQVSAEANQIGNALAKLGVEFGDVVGILAFDGPDWVTSFFGTLKIGAVALGMNT
                     LLTPGEFAHILRDSRARVLIVQDKLLDSIESIRDKAEFLERVVVIGEPRRSSDLRFAD
                     WIGPEPTELRTVETHREDLATLNYSSGTTGEPKGIPHAHKDLPLCAQLWSVNVLGLQE
                     ADRTFAVAKLFFTFGTGGNLIFPWYVGASVVLYPGPPRIATNVLEAIGEFKPTVFYNA
                     PTGYAAILAIDGFNEQYDLSSLRLCVSAGEALPAPIWENWKARTGLDIIDGIGSTEVW
                     HIFISNRPGDIKPGASGKPVEGYEVKLVNEAGEPVKVGEVGNLLVNGETVALSYLHQY
                     ERSKRTFQGEWLFTGDKYVIDEAGFYWHAGRSDDMLKVGGIWVSPVEVESALISHEAV
                     VECAVVGQPDEAGLTKPKAFVVLKEGIAGSDDFARQLIDYCRANMAEYKRPRWIEFVE
                     ELPKTATGKIQRFKLRAG"
     gene            complement(8078..9103)
                     /locus_tag="VI520_01155"
     CDS             complement(8078..9103)
                     /locus_tag="VI520_01155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLF36521.1"
                     /translation="MNQQTLGRYEIRHELGRGGMAIVYLGYDPRIEREVAIKVLPRQF
                     SFDPQFEARFEQEVRAIAKLEHGAIVPVYDFGEVDDQPFIVMRYMSGGSLAARLRLGH
                     LPLPEAARILTRIAPALDRAHAQGIIHRDLKPGNILFDQEGEAYLSDFGIAKLADSTA
                     AYTGSALIGTPAYMSPEQARGEAKVDGRSDVYSLGVILFETLAGRPPYAADTPMGIAM
                     RHVTDPIPRIRRLNPGLPKDLERVIRRALAKAPRNRFQTAAELASALTPIEAGTSPVG
                     AKWTPPIQSETLVGGVAPPDWSGVPPEINKAGPAEAVQLPVESSDRSKKLRRLLLRRR
                     RPPRFRP"
     gene            9139..>9384
                     /locus_tag="VI520_01160"
     CDS             9139..>9384
                     /locus_tag="VI520_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36522.1"
                     /translation="MLSRACRSTAFRVALILTSLALGLAACAAPPAPTPTPVPTPTPE
                     EMLDRSAGAMLAMTSAKFKLTREGEPATLDPNLGLTFS"
BASE COUNT         1741 a         2890 c         3117 g         1636 t
ORIGIN      
        1 tcggagctcc acactcgttt cctgacccct cggctcgacg cccgcctcaa gcagcccagc
       61 ttgccggagg cgttccgcac cgtgctggcc aaacgtgggc tggcggatgt catcgaaatc
      121 ttcaagccgg cggaggtcgc caacccgcac gcggacctgg ccgagctggc ggatctgctg
      181 ctcgagtttg accacttttt ccagttgtgg cgcttcaatc acctgacgat ggtgcagtcc
      241 atgatcgggc gcaaatccgg aaccggattt ctggggccgg agtatctcaa ggagaccgtc
      301 gggctgggca tgcaaggcga ggacgatcga ttgctgcgca ccccgcagat tcggccgcgc
      361 ttcttcgagg agctttggga agcccgcacc cggatgcagg tcggagagca ggaatagccg
      421 ccattgcgaa ccggccagtc aacgcggtga acgagatcga ctgggccgcc tggcgggagg
      481 aatttccggg actgcgccga agcacctatc tcaacacggt ctcgctcggc cagctctccg
      541 ttcgttctcg ggccgcaatg aatcagttca tggacctctg gaccgagtcg ggttccgcgg
      601 cctggtacgc cttctggctc aaagagcagc aggcgacccg agaggagttt gctcggctca
      661 ttggggctga cccgcaagaa gtggcgatca tgcccaatgt ctcctcggcg ctgggggcga
      721 tcagctcctg cctggacttt gcccagcggt ctcaagtggt gacgaccgcc ctcgacttcc
      781 caaccctgac ctatcattgg ctggcaaagg aacgcgaggg gcttgaagcg gtcatcctcg
      841 ccgcgccgga cggaattcag gtcccgctgt cggcgtttga aagggcggtt ggcggcaaca
      901 ccgcgctcat cgctaccagc cgggtttact tcaccaccgg gttcatccaa gatgtcgacg
      961 cactggcgga gctcgcccat cggaacgggg cgctgctgct ggtagacgac taccaggcca
     1021 ccgggcaggt cccgatcgac gtgcggcaaa cgccggtgga cgtgttggtc acgggcgggt
     1081 tgaagtggct actcggcggt ccggggatcg cctatctgta cgtccgccgc gaactgatcc
     1141 agactctcca gccaaccatg accggctggt tcagcaacgc ccgccagttc gattttgatc
     1201 cgcatcgctt ggagttccat gaggatgccc ggcgctttga ggccggcacg ccctcagtag
     1261 cctcgatcta cgcgggcaag gccggcttgg agatcatcaa tgaaattggg gcggtaaacg
     1321 tgcggcaccg caccgcggag ctgagagaag acctcgtggg tcggctgacc gaccgcggtt
     1381 ttcgactgcg gcttccgccg gatcccgaca cacgcagcgg aatcacgatc gtcgagctcg
     1441 aggatccgat ggcggtcacc cgggccctcg ctgaacggcg gatcatcgtc gacaagcgcc
     1501 cgggggcggt gcgactctca ccctatttct acaacacgcc agaagagaac gagctggtcg
     1561 tggaggcgct aactgagatc acccggggca gcgggtagaa gatggcctca gccggtgcgc
     1621 tctttccacc aggcctcgat ggcggcctcc caggacccct ggtaatagcg gtcgaggtag
     1681 gcctgataag aagctatccg ggccgaatag agatcgtgga gcgcgccgag cgcctgcccg
     1741 cccaagagcc gttccatcca ggttgcccaa gcctggaggg cacgctcgac gatcgcccgg
     1801 ccctgctccg ggctgccctt acgcggatcg tcgccaagca ccgcgacctg gccggccggc
     1861 gggagcgaac ccgggggcag cgcagtgaga tcgaccagct cgggtcgata gtgcagcagc
     1921 tgggaagtct cccactctcc ggcgtggtcc aacaaaccgg ggtcctgaag caaagccagg
     1981 ggcgctccgg ccagagtcag gggtccgccg cgatggctga gctggtcggc gacctcggcc
     2041 aggacgatct catggccctg tgcgtagtgc ccgctgacga gacagaccac ccggaaaccg
     2101 gccttcccaa gttcgtgcag cagatcttgc caggcgccgc gcaccacctc ggtccggatc
     2161 gagatcgact gaggatgggg cagggcggtg atgggtagat aggtcgtggg caacagaacc
     2221 gcaccggtgc gaactgcgag ctcctggcaa aaactctcag ccaccaaacc gtccaggccg
     2281 aagggcagat gaggcccgtg ccactcgaga gctccccagg ggacaatggc gaccggaacc
     2341 tcctcaagcc gctggctgag ctggtccggt cgcagttccg cataacgata aacgatggcc
     2401 atggattgca gtctaccatc ctagggcgat aggtcgatgc gaatcaacat agtgggcggt
     2461 gggccggccg gactgttctt cgcgctgctg atgaaaaagc tcgactcgcg gcatgagatc
     2521 accttgtacg agcgggatcc ggccggaaat tcctacggct gggggatcgt gctctccgag
     2581 cgcagcctgg ccaccctgcg cggggccgac ttcgagagct atgcggactt gatcgaagtc
     2641 gccgagctat ggaaaaacgt cgaggtggtt catgccggcg aacggatcac aatcggcggc
     2701 aacgagtttt ccgggctgga gcggatcggt ctgctcaaca ttctgcagcg ccgttgccag
     2761 gccctaggtg tcaagctgga attcgaagcc aacgtcgatg gactcgacgg ggtttcggac
     2821 tgcgacttgc tggtcggggc tgacggcctg aagagcacgg ttcgggagac cgatcgggcg
     2881 cagttcctgc cccggattga ggagcggccc aacaagtaca tctggtacgg tagcccgaga
     2941 gcgttcagcg ggctgaccat gatcttccga ccccatgatg acggcttgta catcgcccac
     3001 gcctaccggt tccagccgaa gttgagcacc ttcatcatcg agtgcgatcc cgacacctgg
     3061 agccgcggcg gtcattccgg ccggaccgag gagcagaccg cggcctacct ggccgaggtc
     3121 tttcgcgacg acctgggtgg cgcaccgctg ttatccaatc gttcgctctg gtacaacttt
     3181 ctgttggtgc ggaacgggaa ttggcaccac cggaacgtgg tgctgctagg tgacgcgctg
     3241 cacagcgtcc acttctcgat cgggtccggg accaccctgg ccatcgagga cgcgattgcc
     3301 ctggcggggg ccttggccca ggaagcgagc gtggctgccg ggctcgaggc cttcgagcgg
     3361 ctgcgcaaac cgcgggtgga ctccctgcag gacgcggcaa ttgacagcct ggtctggctc
     3421 gagaacgtga agggggacct cgatctccat ccacttccct tcgccttcag actgatgagt
     3481 cgcagcaacc gggtgaacta ccgccggctc aagcagcagg atccgggctt tgtggcccgc
     3541 tacgagtcct ggcgctggga gaacgagggt ccgatccatc gggaattcct ggacctgttc
     3601 cagaagaagg cctacggcca tgtggctaca ttaatgaagg acggccgccc gcacgtcaca
     3661 ccggtgtatg tcgacttcga tggtcgttac atccttatca actcgaccaa ggggcggcag
     3721 aaggacctga acatggaacg gcggcgcaag gtggctctcg agatcccgga tcccgatcag
     3781 ccgctgcgct atctggggat ccaaggcgaa gtggtcgaga tcaccgaaga gggtgccgag
     3841 gcacacctgg acaagttggc tcagcgttac cacggacggg agcgctaccc cgcctcgtgg
     3901 aagtttccgc gggaagtgcg gcgaatctac aagatccgtc cagataaggt aattcactgg
     3961 gacccgtttg gcggctggtg aactcgccag aacgagaaag ggaataggga ggcgcgtggc
     4021 tgaacacacc ttcgaggtcc aggacgggat cggggtactg actttcaacc ggccggacgt
     4081 gctcaatgcc ttgacgcttg agctctacgc cgagattatc gagctgttgg ggaagctgca
     4141 gttcgacgaa caggtcaaag tgctggtgct caccggcgcc ggacgggcct tctgctccgg
     4201 cggcgacgtg cacaagatca tcggcgagct gttggcgaag gacatgcggg cccatctgga
     4261 gttcacccgc atgaccggca cggtggtgcg caacatgcgc tcgctcgaca aaccgattat
     4321 tgccgcggtg aatggggtcg ccgccggggc gggggccgtg atcgcgctgg ccagcgatct
     4381 gcgggtcgtg gccgagtcgg cgaagtttgc gtttctcttt accaaagtgg gattggccgg
     4441 cgcagacatg ggcgcagcgt acctgctgcc gcgggtggtc gggatggggc gcgccgccga
     4501 gatcctgatg atgggggaga atatcgacgc cgccagcgcc cagcgaatcg ggctggccaa
     4561 ccgggtggtc cccgatggcg agtcgcttcc agtggcactg gagtgggcaa gaaaactggc
     4621 cgccgggccg agcctggccc tggcgaccac caaacgaatg ttggagaacg agtggaacat
     4681 ggggctgctg gccgccctgg agagcgaagc ccaggcgcag gccctgatgc tgatgggcga
     4741 cgatcaccag gccttttacg aggccttcaa gcagaaagag ccaccccggt tcaccgggag
     4801 atagccgaga tgaccccggc aattaacccg cccgagctcg gccgcccttc gggcttcagc
     4861 tacgcgttcg acgcccgcgg cgagcggact gtgcacttct ccggccacac cgctctggat
     4921 ggctccggaa tgatcgttgg gccgggcaac cttgcggccc aattcgagca gaccctgaac
     4981 aatctgcgag ccaccgcggc cgccgcgcag gtcgagctga aagacctggt caagctcact
     5041 atctttgtga ccgacgtgga ggcctacaag gatcgctcga ccgagattgg ggctgtgtac
     5101 cggcgctact tcggctctca cttcccggcg atgaccctgg tgggagtcac ccggctgtgg
     5161 gaggcggatg cgatgatcga gatcgaagcg gtcgcggttc gctgatccga gtatggactt
     5221 caacctcacc gaaccccaaa tccggatcca gtcgctggtt cgggaattcg cccagcacga
     5281 ggtggcgccg ttggcccgca cgatggacga acaaggccgg atggctccgg agctcgttag
     5341 ccggatggcc gaactgggtc tcttgggtgg gccgctgccg accgaatacg gcgggggagg
     5401 ctgggaccac atgtcgctcg cactggccta cgaggagttg ggtcgggccg actcctcgct
     5461 gcgcggcttc atgaccgtgc ataccagcct ggtttcgctg gcgctgctgg ggatgggaaa
     5521 cgaggcgcag aaagcgagct atctgccgag gctggcagcc ggcgagacca tcggttgcta
     5581 ttgcctgacc gagcccaatg ccggatcgga cgcggccgcg ctggagtgcc aggcccggct
     5641 ggacggcgac cggtatctgt taaatggaga gaagatctgg atcacgaacg gcgggatggc
     5701 cggcctggcg attgtgtttg ccaccctgga accggccgct cgtcaccgtg gtattgccgc
     5761 ctttctggtg atgacggacg ctccggggtt gaggcgagag ccgatgcgcg ggaaagaact
     5821 cgggcaccgg gccgccgacc atgcccggtt cgtgttcacg gactgccgga ttcctgccgg
     5881 ttcgctgcta ggaaagcccg gggagggatt tcaagtcgca atgggagccc tcgaccacgg
     5941 ccggttagga gtagcggccg gggcgctggg ggtggcccag gcctgcctgg atgcctgtgt
     6001 cgagttctcc cgtaaccgcc ggcaattcgg gaagccgatc ggggatttcg ggatgattca
     6061 aacttcgctg gcggacatgg cggtcgaagt tgaggcggcc cgagtgctgg tgtatcgagc
     6121 ggcctggctg aaagaccagg gccggccggc catccgggaa acctgcatgg ccaagcttta
     6181 tgcgaccgag gccgcggccc gggcggccgg ggcggcggtc gtcatccatg gcggccgggg
     6241 ctactccaac gagtacccgg tggagcgtta ttaccgggac atcaaagggc tgcaaatcta
     6301 cgaagggact tcccacattc agcggatcgt gattgcccgc gaattcctgg gtcgcgcggc
     6361 cgggggaccg gggcgagtca gcccgggtac cggcaaggag cagtgaaatg gccgtcaagc
     6421 tcgccgaacg aaccgtgatg aaggccgccg acctgccacc ttactacaat gcggtgcaca
     6481 tcctggagca caacctggtc gagcgagctg agaaggtcgc cctctacagt ccgctgcgca
     6541 acctgacctt ccggcaggtc tcagccgaag ccaatcagat cgggaacgcc ttggcgaagc
     6601 tcggtgtgga gttcggcgac gtggttggga tcctggcttt cgacgggccg gactgggtga
     6661 ccagcttttt cggaacgctc aagatcggcg cggtcgcgct ggggatgaat acgctgttga
     6721 cccccggcga gtttgcccac atcctgcgtg acagccgagc ccgggtcctg atcgttcaag
     6781 acaagctgct ggactcaatc gaatcgattc gggacaaggc cgaattcctc gaacgggtgg
     6841 tcgtgattgg cgagccccgc cgtagttccg acctgcgctt cgccgactgg atcgggcccg
     6901 aacctaccga gctgcggacg gtcgagactc accgggaaga cctggccacc ctgaactact
     6961 ccagcggcac gaccggcgaa cccaagggaa tccctcacgc tcacaaggat cttccactgt
     7021 gcgcccagct ctggagcgtc aacgtgctcg gactgcagga ggccgaccgg accttcgcgg
     7081 tcgccaaact gtttttcacc ttcggcaccg gcggcaacct gatcttcccc tggtacgtcg
     7141 gggccagcgt cgtgctctac cccggcccac cccggatcgc gaccaacgtc ttggaggcga
     7201 tcggcgaatt caagccgacc gtcttctata acgcgcccac cgggtacgcg gccatcctgg
     7261 cgatcgacgg cttcaacgag caatacgacc tgagctcgct ccggctgtgc gtctctgccg
     7321 gagaggcgct gcccgcgcct atttgggaga actggaaggc gcggaccggg ctcgacatca
     7381 tcgatggcat cggctcgacc gaggtctggc acatcttcat ctccaaccga ccaggagata
     7441 tcaagccggg ggcgagcggc aaaccggtcg agggttacga agtcaaactc gtgaatgagg
     7501 ccggtgaacc ggtgaaagtg ggggaggtcg ggaacctgct ggtgaatggc gagacggttg
     7561 cactttccta cctccatcaa tacgagcgaa gcaagcggac tttccagggg gaatggctat
     7621 ttaccggaga taaatacgtg atcgacgagg ccggcttcta ctggcacgcc gggcgttccg
     7681 acgacatgct gaaggtcgga gggatctggg tctcaccggt cgaggtcgaa agcgccctga
     7741 tcagccacga ggcggtggtc gagtgcgcgg tcgtgggcca gccggacgag gccgggctga
     7801 ccaagccgaa ggcgttcgtg gtgttgaaag aaggcatcgc cggcagcgac gacttcgccc
     7861 gccagttgat tgactactgc cgggcgaaca tggccgaata caagcgaccg cgctggatcg
     7921 agttcgtaga agaactcccg aaaaccgcca ccgggaagat ccagcgcttc aagctgcgcg
     7981 cagggtaggt atctcgcaat ctattccgca ggcgtgggga attcctggtc cagggtgggt
     8041 tgtggagtgt cggtgggcac ttcggtcggc tgagacgtca gggtcggaat cggggtgggc
     8101 ggcgcctgcg gaggaggagc cgccgtagtt tcttcgacct gtccgaactt tcgaccggaa
     8161 gctgaacagc ctccgctgga ccggctttgt tgatctcggg aggcaccccg ctccaatccg
     8221 gtggagcgac ccccccgacc aaggtctcgg actggatcgg aggggtccac ttcgctccta
     8281 ctggactcgt tccagcctct atcggagtca gagcactggc caactccgct gcggtctgga
     8341 atcgattccg gggagctttg gccagtgcgc gacggatgac gcgttccaag tccttgggta
     8401 gtcccgggtt gagcctgcgg atccgcggta tggggtcggt aacgtggcgc atcgcgatcc
     8461 ccatcggagt atccgcggca tacggcggcc ggccagcgag cgtttcaaac aggatcaccc
     8521 ccagcgaata gacatcgctg cgaccgtcga ccttcgcctc gccgcgggcc tgctctgggc
     8581 tcatgtaagc gggagtgcca atcagggcgc tcccggtgta ggcggcggtc gagtcggcca
     8641 acttggcgat cccaaagtcc gagagataag cctccccttc ctgatcgaac aggatgtttc
     8701 ccggcttcag gtcgcggtgg atgatgccct gcgcgtgggc ccggtccaga gccggggcaa
     8761 tccgggtgag gatccgagcc gcctccggaa gcggaagatg cccgagccgc agccgggcgg
     8821 ccagcgagcc gccgctcatg tagcgcatca cgatgaaggg ctggtcatcc acctcgccaa
     8881 agtcgtaaac cgggacgatc gcaccgtgct cgagcttagc gatcgcccgc acctcctgct
     8941 cgaagcgtgc ttcgaattga gggtcgaagg agaattgccg cggcaggacc ttgatcgcga
     9001 cttcccgctc aatccgcggg tcgtagccga ggtagacaat ggccatccca cctctgccaa
     9061 gctcgtggcg aatctcatac cggccgaggg tttgctggtt catgcggcga cctggcgcga
     9121 ttatacttag ccgcctccat gctgagccgg gcctgccgca gcaccgcctt tcgggtcgct
     9181 ctgatcctaa cctccctggc actcggcctg gccgcttgcg ccgctccgcc ggctcctacc
     9241 ccaaccccgg tccccacccc gaccccggaa gaaatgctcg accgctcggc cggggccatg
     9301 ttggcgatga cgagcgccaa gttcaagctg actcgcgaag gggagccggc caccctggat
     9361 ccaaaccttg gcctcacgtt cagt
//
LOCUS       DATDPQ010000051         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_800282, whole genome shotgun sequence.
ACCESSION   DATDPQ010000051 DATDPQ010000000
VERSION     DATDPQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800282"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /gene="plsX"
                     /locus_tag="VI520_01165"
     CDS             complement(<1..984)
                     /gene="plsX"
                     /locus_tag="VI520_01165"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HLF36523.1"
                     /translation="MRIVLDAMGSDSAPGPEVAAAAQALARWGEPLTLTGPRQLLENR
                     PEAPAFEVVDAPEILEMTDKPAGAARQKPLSSMAVGMDLVKRGQADAFVTAGNTGGAM
                     ATALFKLGRIPGVKRPALGPVFPVRTGGTVVVDIGANVECKPEYLLQFAIMGSIYAEL
                     VLNRPNPRVGLLSTGEEEGKGNDLVKQTAPLLRAADLNFVGNLEPKDLYAGKADVVVT
                     DGFTGNVFLKTSEAVAALLVEWLRTEIRASPISAAGGWLARPAFKRVSRLLDPSEYGA
                     VPLLGVDGLVFIGHGRSTDRALLNAVRVAREAVSAGLLQALRARLAIALSVV"
     gene            1188..1272
                     /locus_tag="VI520_01170"
     tRNA            1188..1272
                     /locus_tag="VI520_01170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Leu,seq:gag)
     gene            complement(1307..2032)
                     /locus_tag="VI520_01175"
     CDS             complement(1307..2032)
                     /locus_tag="VI520_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36524.1"
                     /translation="MTQQRRPVVVVIVLALFFLAGLVTVLLGGQSAGGSEGTPRAGST
                     ETILILGVDNLAAAQPRLVAVWLATFSAEQRDVILFGQPIDGLVCGSSPTTLQELFVW
                     QPQTGPSSAFLASFGAAEPDGVVVADEAAFTTLIDELHGLDLNGAELDGNQVVAVLRS
                     LYQNPAGLLTSQEQFLISLAQRAAEPPGQVDLTRLTELIPEHAYTSVPPQDLITVYLR
                     LLPLGPASILVFAPQNQAVVCPN"
BASE COUNT          468 a          742 c          726 g          407 t
ORIGIN      
        1 aaccacggag agggcgatag ccagccgtgc ccgcaaggcc tgcagcaatc cggccgaaac
       61 ggcttcgcgg gcgacccgga ccgcgttcaa caaagcccgg tctgttgacc ggccgtggcc
      121 gatgaatacc aggccatcca ctccgagcaa aggcaccgct ccgtattcgg aaggatcgag
      181 cagcctggac acccgcttga aggccggcct ggcgagccag ccgcccgccg cgctgatcgg
      241 agatgcccgg atttcggtcc gcagccactc caccagaaga gcggcgaccg cctcactggt
      301 cttcaggaag acgtttcccg taaacccgtc agtcacgacg acgtctgcct tgcctgcata
      361 gagatccttc ggttccagat tcccgacgaa attgagatcg gcggctctca gcaacggcgc
      421 ggtctgtttc accaggtcgt tgccctttcc ctcttcttct ccggtggata gcaggccgac
      481 ccgcgggttg ggtcgattca agaccagctc ggcgtagatc gaacccataa tcgcaaactg
      541 caacagatac tcaggtttgc actcgacatt ggccccgatg tcgaccacca ctgtgccccc
      601 cgtccggacc ggaaagacgg gtccaagcgc cggccgcttc acccccggga tccgaccgag
      661 cttgaatagc gcggtggcca tcgctccacc ggtattcccg gccgtcacga aagcgtcggc
      721 ctggccacgt ttcacgagat ccattccgac cgccatcgag ctgagcggct tctgcctggc
      781 cgcccccgcg ggcttgtcgg tcatctccag aatctcggga gcatcgacca cttcgaaggc
      841 cggggcttcc gggcgattct ccagcagctg gcggggaccg gttagcgtga gaggttcgcc
      901 ccaacgggcg agggcctggg cagccgcagc gacttccggg cccggagcgg aatcgcttcc
      961 catagcatcc aagacgatac gcatcgatta agccgcgagc ctgcggcttg gcgagcacag
     1021 attggagggc aattgcaacc gcaatctcag ggggtgagtg cccggtcggg gcgtcccgct
     1081 acttccgttg actccccccg gccgttcgct ataataccgc aaatcgagcg agggtcgtcg
     1141 ccgcgcagtg ctggcggaga ggggtggcct tgctttcgat ctgtactgcg ctggtgctgg
     1201 aattggtaga caggcatggt tgagggccat gtgccgcaag gcgtgtgggt tcaagtccca
     1261 cccagcgcac catccacggg ttgccgaaag gcaacccgat tttgtctcaa ttcgggcaga
     1321 cgaccgcttg attctgtgga gcaaaaacca aaattgaagc gggcccgagc gggagtaatc
     1381 ggaggtagac cgtaatcagg tcctgcggcg ggaccgacgt gtaagcatgt tcgggaatga
     1441 gctcggtcaa acgggttagg tcaacctggc cgggaggctc cgccgcccgc tgggcgagcg
     1501 aaatcaagaa ctgctcctgg gaggtcagca gaccggccgg attctgatac aagctgcgca
     1561 gcaccgcgac gacttggttc ccatcgagtt cagccccatt caagtccaat ccgtgcagct
     1621 catcgattaa cgttgtgaag gcggcttcat cggcgaccac gaccccatcc ggctcagcgg
     1681 ctccaaagga cgcaaggaag gcagagctcg gaccagtctg gggctgccag acaaacaact
     1741 cctgcagggt agtgggggaa ctaccgcata ccagtccatc gatcggttgg ccgaatagga
     1801 tgacgtcccg ctgctccgcg ctaaatgtcg ccagccagac cgcgaccaat ctaggttgag
     1861 cggcggccag gttgtcgaca ccgagaatca gaattgtttc ggttgaacct gcccgggggg
     1921 tcccctcact tcctccggca ctctgcccgc ccagaaggac ggtcaccagc ccggccagga
     1981 agaagagggc gaggacaatg acgaccacga ctggccggcg ctgctgggtc atgctcgagg
     2041 aggattgggg cttgattcga acaaatgcgc ccggattgta ctccgtgcgc acggccgcgc
     2101 cacctgagcg gcctcttcag ctcgattccg aggggattat ctacggaggc ttggtatgga
     2161 gcagccttag acgagcccgt tgatgatcgc ctgtccaacg acccggccgg cgaagtaggc
     2221 cgcgaaaccg cccagaaaca ccggtgcata ggtcctgctg agcctcacgg cctcgcggtg
     2281 ttggagggcc cactccgcca acaggacggt ggtcttgagc agtagcagat tgagcaccac
     2341 gat
//
LOCUS       DATDPQ010000052         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2632480, whole genome shotgun sequence.
ACCESSION   DATDPQ010000052 DATDPQ010000000
VERSION     DATDPQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632480"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VI520_01180"
     CDS             <1..978
                     /locus_tag="VI520_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLF36525.1"
                     /translation="REFYTGVRRTVMRDDEVLVDIAFRAPPAGARSTFIKLGLRRAQA
                     IAIVNVAVVLEIEADRVTGAAVTLGSVTPVIVHAAEAEQFLIGKRLDPAGIQQAARLA
                     RQAAAPIDDVRGTARYRAEMVEQCTLRALRSLAAGSERHGYPQRPVTLWGPEEWHWRA
                     ELIESIVHSSETPIETTVNGVRSHFATGQEKSLLRLLREEGGLTGTKEGCAEGECGAC
                     TVSLDGMAVMACMVPAPRAHGAAIVTVEGLADDGRLHPIQQGFIELGAVQCGYCTPGF
                     LMAGAMLLKERPAPSRGEIQEAITGNLCRCTGYYSILEAFERAAELHAP"
     gene            1164..1481
                     /locus_tag="VI520_01185"
     CDS             1164..1481
                     /locus_tag="VI520_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36526.1"
                     /translation="MNPVWRGVGVILIVIMGVAGYAFSGWFLEQNVANQWIYIPPQLY
                     FPAVPAVLGFLGNGNMLRFAVALVFVLTAFAILNFVYALAFPIAPGEFDLPTPKRRPR
                     KRR"
     gene            1505..>2124
                     /locus_tag="VI520_01190"
     CDS             1505..>2124
                     /locus_tag="VI520_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLF36527.1"
                     /translation="MVADSGSVLGKPTARVDALAKVTGEALFPGDIDMPNQVYMKILF
                     AGRPHAVIRRLDTSAAEGMPGVVAVFTAKDVPVNEYGLILPDQPVLCGPGSAKPYADR
                     VRFVGDQVALVVAESEALAAAAVRRIEVDFEDLPVVTDALAARQAGASLVHPDRESNV
                     FSHNRIRKGDVAKGFAAAEVVVESEYRTPAQEHAYLQPEAGLAYID"
BASE COUNT          357 a          643 c          741 g          383 t
ORIGIN      
        1 cgcgagttct acaccggggt ccggcggacg gtgatgcgcg acgacgaagt gctcgtggat
       61 attgccttcc gagcaccgcc agccggcgcc cgctcgacgt tcatcaaatt gggactgcgg
      121 cgggcccagg ccattgccat agtcaacgtg gcagtcgtgc tcgagatcga agcggatcgg
      181 gtgactggtg cggcggtcac cctgggctcc gtgacgccgg tcattgtgca tgccgcggag
      241 gccgagcagt tcctgattgg caagcggctt gacccggcgg gaatccaaca agccgcccgg
      301 ctggcccgcc aggctgccgc gccgatcgac gacgtacgcg ggacggcccg ctatcgggcc
      361 gagatggtcg agcaatgcac cttacgggcg cttcgcagcc tggccgccgg ctcggaacgg
      421 cacggctatc cccaacggcc ggtcactctg tggggtcccg aagagtggca ctggcgggct
      481 gaactcatcg aatccattgt tcactcttcg gagactccga tcgagacgac ggtcaacggg
      541 gtgcgcagtc actttgccac cggccaggaa aaatcgctgc tgcggctgct tcgggaggaa
      601 ggcgggttga ccgggaccaa ggagggctgc gcggagggcg agtgcggcgc ctgcaccgtc
      661 tcgttggacg gtatggcggt gatggcctgc atggtcccgg cccccagggc gcatggagcg
      721 gccatcgtga cggttgaagg gctggcagat gatggccggc tgcatccgat tcagcagggc
      781 ttcattgagc tgggagcggt ccaatgcggc tactgcactc cgggattctt gatggcgggc
      841 gctatgttgt tgaaggagcg ccccgcgccc agccgaggag agatccagga ggccatcacg
      901 gggaatctgt gccgctgcac gggctactac tctatcctcg aggccttcga acgggctgcc
      961 gagctgcatg cgccttgaga ccggttcgga cggcgaacta gcacgatcag gctggtagac
     1021 cgggcgaccg gccgagtcta gaatggggtc ggacctcaag cagcggcgaa agtctggtcg
     1081 ctaaggggca ggaggatcgc atgggcagaa gaggctcttg gcagttccga cgacttgagc
     1141 aagagcggct cgaaaagcgg gagatgaacc ccgtctggcg cggggtgggt gtgatcttga
     1201 tcgtgatcat gggggtggcc ggctacgcgt tttcgggctg gttcctggag cagaacgtcg
     1261 ccaatcagtg gatctacatt ccgccccagc tttactttcc ggcggttccc gcggtgctcg
     1321 gcttccttgg caatggcaac atgctgcgct ttgcggtcgc cctggtcttt gtcctcaccg
     1381 cgtttgccat tctgaatttc gtttacgcct tggccttccc gatcgcgccc ggcgaattcg
     1441 acttgccgac gccgaagcgg cggccgagga agcgccgctg aacctgccgc tccactaacc
     1501 tccgatggtc gctgactcag gctccgtgct cggcaagccg accgctcgag tggacgcgct
     1561 ggcgaaggtc accggcgagg cgctgttccc cggtgacatc gatatgccca accaggtcta
     1621 catgaagatt ctgttcgccg gacgacccca cgcggtcatc cggcggctcg acacctctgc
     1681 cgccgaaggc atgccgggcg tcgttgcggt gttcacggcc aaggatgtac cggtgaacga
     1741 gtacggcctc atcctgccgg atcagccggt gttgtgcggc ccaggatcgg ccaagcctta
     1801 tgccgaccgg gtccgatttg tcggcgatca ggtggcactg gtggtggcgg agagcgaggc
     1861 gctcgcggcg gccgcggtcc ggcgcatcga agtcgacttc gaggatctgc ccgtggtgac
     1921 cgacgcactg gcagctaggc aagccggcgc ttcgctggtc catccggatc gggaaagcaa
     1981 tgtgttctcg cacaatcgga tccggaaggg ggacgttgcc aaagggttcg cggcggccga
     2041 ggtcgtggtt gagagcgaat atcgcacgcc ggcccaggag catgcctacc tccagccgga
     2101 ggccgggctg gcctacattg acga
//
LOCUS       DATDPQ010000053         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2371248, whole genome shotgun sequence.
ACCESSION   DATDPQ010000053 DATDPQ010000000
VERSION     DATDPQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371248"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VI520_01195"
     CDS             complement(<1..272)
                     /locus_tag="VI520_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLF36528.1"
                     /translation="MIRLGTSGFSYDDWVGPVYPADLPRREWLSHYAREFNSVELNVT
                     FYRVPGQKTVAGWVERTPPDFTFSVKANRELTHERGAPDFPGFRSG"
     gene            complement(269..1264)
                     /locus_tag="VI520_01200"
     CDS             complement(269..1264)
                     /locus_tag="VI520_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HLF36529.1"
                     /translation="MRAQRLDRAMPIGDSPLRLAERPDPLPGPGQLLLRVKACGVCHT
                     DLHLAEGDIKPPRFPVTPGHQVVGVVAGLGEGVHAWALGERAGVPWLYSACGGCEFCR
                     RGEENLCEQASFTGFDVDGGYADLLLANADYALHIPAGIPDQQAAPLLCAGIVGYRSL
                     RKADLQPGERIGLVGFGASGHLALQVARHWGCEVYVFTRGSGHRQLATELGAAWVGGV
                     DDEPPSLLDRAVIFAPVGSLVPAMLAKLRSGGTLATNAIHMSPIPELPYRLIDRERTL
                     RSVANATYQDGVEFLKLAAAIPVRPRIKLYGLEQANQALQDLKAARIEGAGVLIP"
     gene            1333..>2113
                     /locus_tag="VI520_01205"
     CDS             1333..>2113
                     /locus_tag="VI520_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF36530.1"
                     /translation="MAHGPAALREAQQLERLLEIGRNLSSNLDLPSLLGSIIAVAAEI
                     TGSEGASILLYDPAARLLRFEAIPPNQNPALKKISVPVDSSVAGWVFSSASPLIINDA
                     GSDARIYREVDRQIRFETRSILGVPLIVKQEPIGVLEAVNKLAGQDYTEPDQVALETL
                     AAQAAIAIENARLLAKLHAANLELNRLDRMKSDFIAIASHELRTPLGLILGHATFLTE
                     LVDEKHRDQIEVIVRSAERLKAIVEDMARISHQEEGQSRVRR"
BASE COUNT          411 a          700 c          635 g          367 t
ORIGIN      
        1 atcccggacc ggaagcctgg gaagtcgggt gcgccgcgtt cgtgggtgag ttcgcggttg
       61 gccttcaccg aaaaggtgaa atcgggaggc gtgcgctcca cccagcccgc cacggtcttc
      121 tgccccggca cccgatagaa cgtgacattg agttcaacgc tgttgaactc cctggcgtaa
      181 tgggaaagcc actcccggcg cggcagatcc gccgggtaga ccggacccac ccaatcatca
      241 tacgagaacc cggaggttcc aagtcgaatc acgggatcaa gactcccgcg ccttcgattc
      301 tggcagcctt aaggtcctgc agcgcctggt tggcctgctc gagtccgtag agcttgatcc
      361 gaggccgaac cgggatggcc gctgcgagct tcaggaattc cacaccgtcc tggtacgtgg
      421 cattggcgac gctgcggaga gtccgctccc gatcgatcag ccgataaggc aactcgggta
      481 tcggactcat atggatcgcg ttcgtagcca gagtgccccc gctgcggagc ttggccagca
      541 ttgcgggaac gagcgaaccc accggcgcga agattaccgc ccggtcgagc aggctcggcg
      601 gctcgtcatc gaccccaccc acccaggccg ccccgagctc ggtcgccagc tgccgatggc
      661 cggagccgcg ggtgaagacg tagacctcac acccccagtg tctcgccacc tgcagtgcca
      721 gatgcccgct ggctccgaaa ccgaccaggc caatccgctc gccgggttgc aggtcggctt
      781 tacgaagaga acggtagcct acgattccgg cgcacagcag gggggccgct tgctgatcag
      841 ggatacccgc cggaatatgc agcgcgtagt cggcattcgc cagcaggagg tcggcgtacc
      901 caccatcgac atcgaagccg gtgaagctgg cttgctcgca gaggttttcc tctccccgtc
      961 tacagaactc gcatcctccg caggcgctgt acagccatgg gaccccggcc cgctcgccca
     1021 gagcccaagc gtgcacccct tccccgagcc cggctaccac gccgaccact tgatgcccag
     1081 gcgtaaccgg gaaccgcggc ggcttgatgt caccctcggc cagatgcaga tcggtgtgac
     1141 agaccccgca ggccttcacc ctcagcagca gttggccggg gcccggcaag ggatccggcc
     1201 gctcagctaa ccgaaggggc gagtcgccga tcggcatggc tcgatccagg cgttgggctc
     1261 tcatcggctc gccgaatgca gaccctcttc ctagaaggcc agaaacctgg ctatgctaga
     1321 atcgaagcag cgatggctca cggaccggcg gcccttcgcg aagcccagca gctcgagcga
     1381 ctgctcgaaa tcggacggaa cctcagctcc aacctggatc tgccctcact cttgggttcg
     1441 atcattgcgg tggctgctga aatcactggc agcgaggggg cctccatcct gctctatgat
     1501 ccggcagctc gcctgttgcg ctttgaagcc attcccccca accagaaccc ggcgctcaag
     1561 aagatcagcg tcccagtcga ctccagcgtt gccggatggg ttttcagttc cgcctccccg
     1621 ctgatcatca acgacgccgg gtcggatgcc cggatctacc gagaagtcga tcggcagatc
     1681 cgttttgaga cccgctcgat tctgggcgtg ccgctcattg tgaagcaaga accgattggc
     1741 gttctggagg cagtcaacaa gcttgccggt caggattata ccgagcccga tcaggtcgct
     1801 ttggaaaccc tggccgccca ggctgcgatc gcgatcgaga acgcccggct gctggccaag
     1861 ttgcatgcgg cgaatctgga acttaaccgg ttagaccgga tgaagtcgga ttttatcgcg
     1921 atcgcctccc atgagctgcg taccccgctt ggcctgatcc tcggccacgc gacattcctg
     1981 accgaactgg tcgatgaaaa gcatcgagat cagatcgaag tgatcgtccg cagcgcggaa
     2041 cggttgaagg ccatcgtgga agacatggcc cgcatctcac accaagagga gggtcagtca
     2101 cgagtgcgcc gcc
//
LOCUS       DATDPQ010000054         4746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_20674, whole genome shotgun sequence.
ACCESSION   DATDPQ010000054 DATDPQ010000000
VERSION     DATDPQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20674"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VI520_01210"
     CDS             complement(<1..733)
                     /locus_tag="VI520_01210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF36531.1"
                     /translation="MSYRLCADCGLVYQSPRMSEAALQQYYQAEYVAQHQRATGVSEK
                     ELKVQTGRARNLVRFLRPQLASVARHLDIGCSAGLLLEAVRGTYGCESVGVEPAEVYR
                     NYCAARGLTVSSDLDRLAEAGNDRFDLVSLAHVLEHLADPVGYLADLRNTWLRPGSKL
                     LVEVPNLFGHSSLETPHLVCFHSGTLRRTLAVAGYRVVRLIRHGEPRSRLIPLYLTAI
                     AEPAAGMVSRESVRFGAGGVRRRRRF"
     gene            complement(868..1815)
                     /locus_tag="VI520_01215"
     CDS             complement(868..1815)
                     /locus_tag="VI520_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36532.1"
                     /translation="MRGVTLFSAPKPFLEPHIGLIQINAASSWMSLGPEVEVLWIGDE
                     PGLAKAAAELGVRQLAPVGRNTSGTPLVSSVFELAQQAAHHDLLGYVNADILLLDDFV
                     KSVDAVADRFHRFLIVGQRWDLAIGSKLVFTGDWSSDIRKRLGQEGQLHPPAGSDYFV
                     FPRGSLGRMPEFAIGRAGWDNWMIYAARRMGLPVIDASQAITAVHQNHDYAHLPNGKP
                     HYGLPESEQNVRLAGGHQTIFTLADATWQIAGGEIEKIRWPRGGVLRALEAAITSRAG
                     SAGLLRVVRILFHPLEFLRSRSGIAARWIRSRIGARGDA"
     gene            complement(1833..2633)
                     /locus_tag="VI520_01220"
     CDS             complement(1833..2633)
                     /locus_tag="VI520_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLF36533.1"
                     /translation="MTANLAKVTNSGRPKVVAIVQARMASSRLPGKVLADIGGQPMLV
                     RVVERSRRASRLAQVVVATSTENDDDPIAAVCAERAYDLYRGDSLDVLDRYVQTARRF
                     EAEVIVRLTGDCPLIDPEVIDQTVKAFLEAAPPVDFAANRLPSDRSFPIGLDTEVCSR
                     STLELAWREAAAPHHREHVMPFLYENPDRFRTLLIHNEVDYGHLRWTVDTQEDLALVR
                     QIYAYFAGKDHFSWMEVLELYERQPQLAEINASVAHKTELDLDPRWNP"
     gene            complement(2648..4258)
                     /locus_tag="VI520_01225"
     CDS             complement(2648..4258)
                     /locus_tag="VI520_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF36534.1"
                     /translation="MKTPALEARQGRQLRSIHWDRLAQLAATSRTGFVAITTGLVLFG
                     VIRLVQATPYGPGLNGDSFYYYTGAENLAAGRGFGRIAGSGLFVPTTHFPPGYSGAMA
                     FLQLMGIDKLDSGNLVNLLSLAGLVLVLAFALRRESGSNIPAYAACAILVVSPVMLES
                     YVWAMSEPFYLLLSFAGLYGLVLYLRNRRPELLVASAALLGLAWITRYVGVALVGTAL
                     AALMVQRVPWRKRLISVGMFAAISCLPMLVWLGRNALLTGNPANRRLLWHPIGYSQLK
                     AFALHGLEWFVPAELVHGGWQALAMILVIAVLVGVFVFRLAKRGQLSARFRRTTLPVV
                     LVLHGVLYLAALAVSISLLDPATQIGGRILLPIYVSVIILGGLLGWDIWRHQGWIVRL
                     GILSAGMFILIWNGVEQAQQAISIGEYGLGNAAPSIAESQTLAAVEELADVPIASNGM
                     ARLYFWADRNAYAIPWRVDLETGEPRISYADEVRNLKDVLCRDGGALVLFGPENLLSE
                     QAALSDLTAGFTRSGKYSDGEIYRCEGP"
     gene            complement(4293..>4746)
                     /locus_tag="VI520_01230"
     CDS             complement(4293..>4746)
                     /locus_tag="VI520_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF36535.1"
                     /translation="LLLLGSGSLETEIQNRIALGGAEHQVHFAGQVEFARLPEMYRAA
                     DLYVSASHSDGSSVSLLEALACGLPALVSDIPGNRDWVEPEVNGWWFRDGSGKDLARL
                     LRHAVSQAANLPAMGRSSRRVAETRADWTQNFARLAEAYGLAFRETGR"
BASE COUNT          973 a         1542 c         1408 g          823 t
ORIGIN      
        1 caaaccggcg ccgccgccga acgcctccgg ccccgaaccg gaccgattca cgggagacca
       61 tcccggccgc gggctcggcg atcgcggtca ggtacaaggg gattagacgc gaccgaggtt
      121 caccgtgccg gatcaatcga acgacccggt agccggccac tgccaacgtc cggcgaagcg
      181 tgccgctgtg gaagcacacc aggtgcggcg tctccagcga gctgtggccg aacagattgg
      241 gaacttcgac caggagtttc gagcctggcc gcagccatgt attccgaagg tcggcaaggt
      301 acccgaccgg gtcggccagg tgctccaata cgtgggccag gctcaccagg tcgaagcggt
      361 cattgcccgc ctcagccagc cggtctaagt cagaggaaac cgtaaggcct ctcgccgcac
      421 agtaattgcg gtagacctcg gccggttcga cgccgacgct ctcgcagcca taagtccctc
      481 gcacggcctc caacagcaag ccagccgaac atccgatgtc gaggtggcgg gccaccgatg
      541 cgagctgcgg acgcaggaat cgcacgagat tgcgagcccg cccggtttgc actttcagtt
      601 ctttctcgct caccccggtt gcccgctgat gctgagcgac gtactccgcc tggtagtact
      661 gctgcagcgc cgcctccgac attcgcgggg actgatagac cagcccgcag tccgcacaca
      721 ggcggtagga cacagacttt gatccctctg agacactttc aaatgattcg gcctgggtgc
      781 tcaggcatag cgggcaggca ccgactgtta caacccccgg ttcctgctcg gctctgatcg
      841 acttgaccgc agcactctgg ctggacacta cgcgtctcct cgtgccccga tccgggaccg
      901 gatccagcgt gcggcaatcc cgctgcggct ccgcaagaat tccagcgggt ggaagagtat
      961 ccgaacaacc cgcagcaggc ccgccgaccc agcccgggag gtaatcgcag cctccagcgc
     1021 ccgcagaacc ccgccccgcg gccatcggat cttctcgatc tccccgcccg cgatctgcca
     1081 ggtcgcatcc gctaatgtga agatcgtttg atgtccgccg gccagccgca cgttctgctc
     1141 gctttcgggc agtccataat gcggttttcc gttcggcaaa tgagcgtagt catggttctg
     1201 gtggacggcg gtgatggcct gactggcgtc aatgaccggc agccccattc gacgggcggc
     1261 gtagatcatc cagttgtccc atcccgcccg cccgatagcg aattcaggca tcctgcctaa
     1321 cgatccgcgg ggaaacacga aatagtcact cccagcgggt gggtgaagct gaccctcctg
     1381 cccgagccgc tttcgaatgt cgctgctcca atctcccgtg aagaccagct tcgagccgat
     1441 cgcaaggtcc cagcgttggc ccacgatcag gaatcggtga aaccggtccg cgactgcgtc
     1501 caccgatttc acaaagtcat ccaggagcag gatgtctgcg ttgacatacc caagcaagtc
     1561 gtggtgggcg gcctgttggg ctaactcaaa cacagagctc accaacgggg taccgctagt
     1621 attccgaccg accggcgcca gttgcctgac accgagctca gctgccgcct tcgccagccc
     1681 cggctcgtct ccaatccaca acacctccac ctcaggcccc agggacatcc aggaactcgc
     1741 cgcattgatc tgaatcaacc cgatgtgggg ttccaagaag ggcttggggg ccgagaacag
     1801 agtcactccg cgcatgggtt cgagcgccgg gcctatggat tccagcgcgg atccagatcg
     1861 agctcagtct tgtgagcgac cgatgcattg atttccgcca gttggggctg tcgctcgtag
     1921 agttcgagca cttccatcca agaaaagtgg tccttcccgg cgaagtaagc atagatctgc
     1981 ctaaccaggg cgaggtcctc ctgggtgtct acggtccagc gcaggtggcc gtagtccact
     2041 tcgttgtgga tcagcagggt tcgaaatcga tccgggttct cgtagaggaa gggcatcacg
     2101 tgctctcgat gatggggcgc ggctgcttcc cgccaggcaa gttcgagagt cgaccggctg
     2161 cacacttcgg tgtccaagcc gatcggaaag ctccgatcgg aaggaagccg gttggccgcg
     2221 aagtcgaccg gcggcgcggc ttccagaaac gccttcaccg tttggtcaat cacttccgga
     2281 tcgatcagcg gacagtcgcc agtgagtcgc acgatgacct ccgcctcgaa tctgcgggcg
     2341 gtctggacat atcggtcgag cacatccagc gaatctcccc ggtagaggtc gtaggcccgc
     2401 tcggcgcaga ctgcggcgat cgggtcgtca tcgttctcgg tgctggtggc cacgaccacc
     2461 tgtgccagac gactcgcccg gcgcgaccgt tccaccactc ggaccaacat cggctggccg
     2521 ccaatatcgg caaggacctt gccgggcagc cggctggagg ccatccgagc ctgcacgatc
     2581 gcgacgacct ttggtcggcc tgagttagtc actttcgcca aattggcggt cattgtgatg
     2641 atgtgcctta cggcccttca caacggtaga tttcgccatc tgaatacttg ccagagcggg
     2701 tgaagccggc ggtcaaatcg gaaagggccg cttgctcgga cagtaggttt tccggcccga
     2761 agagtacgag cgccccccca tccctgcaaa gaacatcctt gaggtttcgc acctcgtccg
     2821 cataggaaat cctgggctcc ccggtctcca gatcaactct ccatggaatc gcataggcgt
     2881 tgcggtcagc ccagaagtag agccgtgcca tcccattgga agcaatcggc acatccgcaa
     2941 gctcctccac tgcggccagg gtctgagact ccgcgattga gggcgcagca ttgccaagac
     3001 catattcgcc tatcgaaatc gcctgttggg cttgttcgac tccattccat atgaggatga
     3061 acattcctgc cgagagaatc cccaggcgca cgatccatcc ctggtgtcgc cagatatccc
     3121 agccgagcag gcccccaaga atgatcacgg acacatagat cggcaagaga atgcgaccgc
     3181 cgatctgagt tgccggatcg aggagcgata ttgaaacggc cagcgcggca agatagagaa
     3241 cgccatgcag gactagaacg accgggaggg tggtccgccg aaaacgcgcc gaaagctgac
     3301 cacgcttggc gaggcgaaag acaaacaccc cgactaggac ggcgatcaca aggatcatgg
     3361 ccagcgcttg ccacccgccg tgaacgagct ccgcagggac gaaccattcg agcccatgca
     3421 gagcgaacgc cttaagttgc gaataaccta tcgggtgcca gagcagcctt cgattcgccg
     3481 gattgccggt aagcagcgca ttgcgaccaa gccagacgag catgggaaga cagcttatcg
     3541 cggcaaacat gccgacgctg atcaggcgct tccgccatgg aactcgctgg accatcaagg
     3601 ctgccagtgc cgtgccaacc agcgcaactc ccacgtacct cgtaatccaa gcgaggccga
     3661 gcaaggcagc cgacgccacc agcaattcgg gacgacgatt cctgaggtaa aggacgagcc
     3721 catagagtcc ggcgaaggag agaagaaggt agaagggttc gctcatggcc cagacatacg
     3781 actccagcat gactggtgac accacgagaa tcgcgcacgc ggcgtaggcc ggaatgttcg
     3841 atccggattc ccttcgtaac gcaaacgcga ggacgaggac caatcccgca agagacaaca
     3901 ggttgacgag gttcccgctg tccagtttgt caattcccat cagctgcaga aacgccattg
     3961 ctcctgaata acccggcggg aaatgggttg tcgggacaaa caagcccgag ccagcgattc
     4021 ggccaaatcc ccgcccggcc gcgaggtttt ccgcgccggt gtagtaatag aatgaatccc
     4081 cgttcagccc gggtccatag ggagtggcct gaaccagccg aatcactccg aaaagcacca
     4141 gccccgtcgt aatcgcgacg aacccggtcc gactggtcgc cgccagttgt gcgaggcgat
     4201 cccagtgaat gctgcggagt tgcctgcctt gcctggcttc gagcgcaggt gtcttcatcc
     4261 cggctccatg ttaggttcag cccgttcggg gatcatcggc ccgtctcccg gaaggccagg
     4321 ccgtaggcct ccgccagtct ggcgaagttt tgagtccaat ctgcccgggt ctcagcgact
     4381 cgccgcgagg aacgccccat ggcgggcagg ttcgcggctt ggcttacggc gtgccgcagc
     4441 agacgggcga ggtcttttcc gctcccgtcc cgaaaccacc agccattgac ctccggctcc
     4501 acccagtccc ggttgcccgg gatgtcggag accagcgccg gcaggccgca ggccagcgcc
     4561 tccagcaagg aaaccgagct gccatcgctg tgggaggcgc tgacgtagag atcggccgcc
     4621 cggtacatct ccggcagacg ggcgaactcc acctggccgg cgaaatgcac ctggtgttcg
     4681 gcgcccccta gcgcgatccg gttctggatc tcggtctcca ggcttcccga gcccagcagg
     4741 aggagc
//
LOCUS       DATDPQ010000055         5777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3681908, whole genome shotgun sequence.
ACCESSION   DATDPQ010000055 DATDPQ010000000
VERSION     DATDPQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681908"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VI520_01235"
     CDS             <1..1182
                     /locus_tag="VI520_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36536.1"
                     /translation="GFRAAGIATGLVWAIAPWSVTFAIGGLETSVLVGLATATFYLHL
                     THRPVGAAAAGALALLTRPDALLFLGPLAIGRALQIRKSAPPGRPGELFAFGAPLVAW
                     LAWSQVYFGSPIPHSLFAKSVAYQIPAEAGLVRLLQHYATPFLEHELLGAGAIRVGIV
                     LYPALFLLGALSATRANREGWQILAYPGLYLLAFAIANPLLFRWYLTPPLPMYFLGLF
                     IGASRVSKDLRSRVPLLGFATLALASTASAWDWTPDHGARRPAPKMAFIELELLYEHT
                     AELVETRLSSNQVLAASDIGALGYYTGARILDMLGLVSAEASQYYPAPPSMYVINYAI
                     PPDLVRDLKPDMVVMLEVYGRNGLLLDPAFQESYQLVDELPTDIYGSRGLLIFVRKES
                     E"
     gene            1179..2489
                     /locus_tag="VI520_01240"
     CDS             1179..2489
                     /locus_tag="VI520_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36537.1"
                     /translation="MTPNRWRSWAPAVGLLLLTGYQLWGVALVPFHPDESSLLFQSRD
                     FEAIFRSPTSLAWSADRQPDPELSYRLLNAPFPKYVLGFGRWLAGVDGAEVSVDWDWT
                     QDWIANLGAGALPTPRALLAGRIASSSMLPFAALFLYLCGKQLGGPWVGGLAAALLGL
                     NALVLVHGRRAMAEGTLIFAICFALWSLLGPRRRPWLSGLGIGLALGAKHSALALLPV
                     GILAVLWGAGPSQPIRQKLSSVGMFVLVLMAVTLALNPVVWSEPASALREIFHVRMSL
                     VQDQVQAQAIAAQQAIAVPLRGIDRLAVLIGHLFLAPPQFQEIGNYRAELDSSVAHYL
                     AVPGHALLRGRTLGGLSLGLTLAGLFFGLATLRTGSRSVSRTAALLLISTAALALTLY
                     IGIPLPYQRYYVPLVPQVCLWSAFAVIRLGQEMKKLPFIRAALG"
     gene            complement(2503..3747)
                     /locus_tag="VI520_01245"
     CDS             complement(2503..3747)
                     /locus_tag="VI520_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLF36538.1"
                     /translation="MAKPKKKWRRTDPAVDEDSSPEEVAAQEEESQALEHLLEQGKER
                     GFVTIDEILAVFPEAERDLDQLEEVYSSLLAAGIAYLDDVSDGEDPADEDLAEIEASQ
                     TRDKNDLAAIDADDSVGLYLKEVGRVPLLTAEQEVSLAKRIERGRRARESMARGGISE
                     SRRTHLRLYIEDGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRAAK
                     KFDYRRGHKFSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINRLLRTSHQLTQFLG
                     RDPTTEELADALSVTPKKVENMIQVARRPISLETPTDEEEDSVLADFIEDEESPAPAE
                     AVTKSLLREQLRDVLHTLPPREVRILQLRYGLHDGQSYTLEEVGRKMGVTRERVRQIE
                     AQALSRLRHPSHRRKLVDYLRE"
     gene            complement(3836..4507)
                     /locus_tag="VI520_01250"
     CDS             complement(3836..4507)
                     /locus_tag="VI520_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HLF36539.1"
                     /translation="MIRRNTWITLGVFGLVLVAAILVRQQQQAEPAPTPSASIAPFWQ
                     VESSEIVSLTVEDLQAGTVLQLERDPEDLWQIVLPETLPADPARVERAVAWLSAPTPR
                     AEVLDAIDLTAFELDQPQFRVEIVLSTGERHEFSVGRPAPTGDSRYASTPGRAGVQIL
                     SLVGLEEVLNLAADLLPTPTPEPTPSPSLAPETGTPTTPDPSPGPETTQTPTPGGGPE
                     VTPTP"
     gene            complement(4511..>5777)
                     /locus_tag="VI520_01255"
     CDS             complement(4511..>5777)
                     /locus_tag="VI520_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HLF36540.1"
                     /translation="RIDLTEDKEFSLSPETELLLSELSEPVKLTGFYTPARASSRDQI
                     RPLLDEFVSKSNGLVSYEFIDPQSNPLAADLFGVTRDASMAATLGEDSHLIEFPSERE
                     ITSAIVRLTNPEDRVVYFLTGHGELELAETGDIGLSQLKSSLESKNYGVQALNLLVEG
                     EVPGDASVVIAAAPRTQLTEPELQLINDYLISGGSSIIMLEPSAVTQLDPADDTLNAY
                     LLEQWGVSARDDFVVDLGSSLNYVGIGLSYGSHPITERVSSFFTVYPSARSIKVSESV
                     ELTRTVTQLVQTSERSWGETDFAAIAQERGIQYDEQIETVGPLTLAAALEDSRQGARL
                     VVIGDGDFASNGFFFEGGNGDFMVNSIDWAARQEDLIDITPRQRTVRQVLPATRSTVT
                     LLVFGTTVVIPGGIVLAGGWVWWNRRKRS"
BASE COUNT          992 a         1844 c         1733 g         1208 t
ORIGIN      
        1 ggcttccgcg cagccgggat cgcgaccggg ttggtgtggg ccattgctcc atggtcggtt
       61 acctttgcaa tcggcggcct cgaaacgagc gtcctggttg ggttggcgac cgcgacattc
      121 tacttacatc tcacccaccg gccagtcggc gctgcggcgg ccggcgctct cgccttgctg
      181 actcggcctg atgccctgct ctttttagga ccgctcgcga tcgggcgtgc cctgcagatc
      241 agaaagtcgg cacctcccgg gcgccccggc gagctgttcg catttggagc tccgcttgtg
      301 gcctggctgg cttggagcca ggtgtacttc ggatctccca tccctcactc cctattcgct
      361 aaatcggtcg cctatcagat cccagccgag gccgggctgg tccgcctatt gcaacattac
      421 gccacaccct tcctcgagca cgaattgctt ggggcaggtg cgatccgggt tgggatcgtc
      481 ctgtatccag cgctcttctt actcggagcc ctcagcgcga cccgagcgaa ccgggagggc
      541 tggcagatct tagcctaccc agggctgtat ttgttggcgt tcgccattgc caatccgctc
      601 ctgttccggt ggtatctgac gccgccgctg ccgatgtact tcttgggtct tttcattgga
      661 gcctcgaggg tctcgaagga cctccggagc cgagttccgc tactcggatt cgcaacgctg
      721 gccctcgcct ctaccgccag cgcttgggac tggactcctg atcatggagc cagacgacct
      781 gcgcccaaaa tggcgttcat tgaactggag ctgctttacg agcacacggc agaactcgtc
      841 gagactcggc tttcgagcaa tcaggtgctg gccgcttctg acatcggtgc gttgggatac
      901 tacacggggg ctcggatcct cgacatgctc gggctggtgt ccgcggaggc gagccaatac
      961 tatccggccc cgccctcgat gtatgtgatt aactatgcga taccgcccga tctggtgcga
     1021 gaccttaagc cagatatggt cgtcatgctt gaagtctacg gccgtaacgg cctgctgctc
     1081 gatcccgctt tccaggagtc gtatcaactc gtcgatgagt tgcccaccga tatatatgga
     1141 agccgaggct tgctgatctt cgtccgcaaa gagtctgaat gacccccaat cgctggaggt
     1201 cgtgggctcc ggcggtcgga ctgctgctgc tgaccgggta ccagctgtgg ggagtggcgc
     1261 tggttccgtt ccatccggac gagagctccc tgcttttcca gagccgcgac ttcgaggcaa
     1321 tttttcgctc cccgacctcg ctggcctggt cagccgaccg ccagcccgac ccggagctga
     1381 gctaccgcct gctcaacgcg cccttcccga agtacgtcct cggctttggt cgctggctcg
     1441 ccggagtcga cggggcggaa gtgtccgtcg actgggactg gacccaagac tggatagcaa
     1501 accttggcgc tggcgccctc cccactccga gggcgctgct ggccggtcgg atcgcgagca
     1561 gcagcatgct gccgttcgcc gcgctgttcc tctacctgtg cgggaaacaa ctcggcgggc
     1621 cgtgggtcgg cgggttggcg gccgccttgc tggggctcaa tgcgctcgtg ctggtccatg
     1681 gccggagagc gatggccgaa ggcacgttga tcttcgcgat ttgtttcgcg ctctggagcc
     1741 tgctcgggcc ccgcaggcgg ccgtggctat ccgggctcgg gatcggattg gcgctagggg
     1801 ccaagcactc ggctctggct ctgctgccgg tcgggatcct cgcggtgctc tggggagcag
     1861 gcccgtccca accgatccgg cagaagctca gctcggttgg aatgtttgta ttggtgttga
     1921 tggcggtcac cctcgcgctg aatccggtag tttggtctga gccggcctcc gcgctcagag
     1981 agatcttcca tgttcggatg tcgctcgtcc aggatcaagt gcaggcccaa gcgatcgccg
     2041 cgcagcaggc gattgcggtc ccgctacgcg ggattgatcg actcgcggtg cttattggcc
     2101 acctgttcct ggcaccgcct cagttccaag agattggcaa ctatcgagcc gagttggatt
     2161 cgagcgtcgc ccactacctg gcggtccccg gccatgcgct gctgcggggc cggaccttag
     2221 gaggcctgag cctcggcttg acgttggccg gcctgttctt cggtcttgca acgctgcgga
     2281 ccggctcgcg atctgtgagc cggacagccg ccctactgct catcagcacg gcggcccttg
     2341 cgctcaccct ctacatcggg atcccgctcc cgtaccaacg ctactacgtg cctctggttc
     2401 cgcaggtttg cctctggtcg gcgttcgccg tcatccgatt gggacaagaa atgaaaaagc
     2461 tgcccttcat cagggcagct ttaggctaga acgctcagcc agctactctc tcaagtagtc
     2521 gactagtttt cggcgatggg agggatgccg cagtcggctc agggcctggg cttcgatctg
     2581 gcgtacccgc tcacgggtca cgcccatctt gcggccgact tcttccagag tgtagctctg
     2641 cccgtcatgc aagccgtagc gaagttgcag gattcgcact tcccttggcg gaagcgtatg
     2701 cagcacgtct cgcagttgct ccctcaacag actcttcgtc accgcctcgg ccggcgcggg
     2761 agactcctcg tcttcgatga aatcggccaa caccgaatct tcctcttcat cggtgggggt
     2821 ctccagcgaa atcggccggc gggccacctg aatcatgttc tcgaccttct tcggagttac
     2881 gctcagcgca tccgccagct cctcggtcgt tggatcacgg ccgagaaact gggtcagctg
     2941 atgcgaagta cggagcagcc ggttgatctg gtcgcccatg tgcaccggca ctcgaatcgt
     3001 tcgcccttga tccgcgatcg cccgggtaac cgcctggcgg atccaccagg tggcgtaggt
     3061 cgaaaacttg tgcccgcgcc gatagtcgaa cttcttcgcg gcccggatca atccaatatt
     3121 cccttcctga atcaggtcta ggaatggaac tccccgcccc atgtatttct tggcgacgct
     3181 gatgacaagc cgcgagttgg cggtgatcag gtgttcccgg gctgcccagc cgtcctcgat
     3241 gtacaggcgc aggtgggtcc gccgcgactc agagatcccg ccccgagcca tggattcgcg
     3301 tgcccgacgc ccgcgctcga tccgcttggc gagcgagacc tcctgctccg cagtcaacag
     3361 cgggacccgg ccgacctcct tcagataaag accgacgctg tcgtcagcat caatcgcggc
     3421 caaatcgttc ttgtcgcggg tttgagaggc ctcgatttcc gccaggtctt catccgccgg
     3481 gtcttcgcca tctgaaacgt cgtccaaata agcaatgccc gccgccagca ggctggaata
     3541 gacttcctcg agctgatcca ggtcacgctc agcctctggg aagacggcca gaatctcgtc
     3601 gatcgtgacg aagccccgct ccttcccctg ttccaggaga tgctccaggg cctgactttc
     3661 ctcttcctgg gcggctacct cctcggggct ggaatcctcg tcaaccgcgg gatccgttct
     3721 tcgccacttc ttcttcggct tggccaattg gcttccttcc ttctcacctt ccggcgcagt
     3781 tcagcttaat gactttcggg gtcggaatca acacccaaag gggaaactcc tctgctcagg
     3841 gcgtcggtgt gacctccgga cccccacctg gggtcggagt ctgcgtcgtc tcgggtcccg
     3901 ggctagggtc gggcgtcgta ggagttcccg tctcaggtgc caggctcggg ctgggagtcg
     3961 gctctggagt cggagtcggc aggaggtcag ccgccagatt caagacctct tccagtccga
     4021 ccaggctcag gatttggacc cccgcccgtc ccggagtgga ggcgtaccga gaatcaccgg
     4081 tcggcgccgg acgaccgacg ctgaattcgt ggcgctcgcc agtgctcaat acaatctcga
     4141 ctcgaaattg aggttggtcg agctcaaagg cagtcagatc aattgcgtcc aacacttcgg
     4201 cccgcggggt tggcgccgaa agccacgcca cggcccgctc tacccgggct ggatccgcag
     4261 gcaaggtctc tggtagcacg atctgccaca ggtcctccgg atccctctcc agttgcaaga
     4321 ccgtgccagc ctgcaggtcc tcgactgtca gactgacgat ttcactgctt tcaacctgcc
     4381 aaaacggagc tattgaggcc gagggagtcg gcgcgggctc ggcctgctgc tgctggcgga
     4441 ccagaatggc cgcgaccagc accaatccga agacccccaa cgtaatccag gtattcctgc
     4501 ggatcatggg ttaggaccgc ttgcgccgat tccaccagac ccacccgccg gccaggacga
     4561 tcccacccgg aatcacaacg gtggttccga agaccaagag ggtgacggtt gatcgggtcg
     4621 ccggcagcac ctgccggacc gtgcgctgac ggggagtgat atcgatcaag tcttcctggc
     4681 gcgcagccca atcgatgctg ttgaccatga aatcgccgtt accgccctca aagaagaacc
     4741 cgtttgatgc gaagtcgcca tcgccgatca ctaccaaccg ggctccctgc cgactatctt
     4801 cgagcgcggc cgccagcgtt agcgggccga ccgtctcgat ttgctcgtcg tattggatcc
     4861 cgcgctcttg ggcgatcgcc gcgaaatcgg tctctcccca agagcgttcg ctggtctgga
     4921 ccagttgggt gacggttcgg gtaagctcga cggactccga tactttgatg ctgcgggccg
     4981 aagggtacac ggtgaagaaa ctcgacaccc gctccgtgat cggatgggat ccataactta
     5041 agccgatgcc cacataattg agcgaagacc ccaggtctac gacaaagtca tcccgggccg
     5101 aaacccccca ctgttcaagc aggtacgcat tcagcgtatc atctgccgga tcgagctggg
     5161 tgaccgctga gggctcgagc atgatgatcg aggaacctcc ggagattaga taatcgttga
     5221 tgagctggag ttccggttcg gtcaattggg ttcgcggggc ggcagcaatt actacgctcg
     5281 cgtcacccgg gacctcgccc tcgacgagca gattgagggc ctgaacgccg tagttcttgg
     5341 actccaggga actctttaac tgggatagcc cgatgtcacc cgtctccgct aattcgagtt
     5401 cgccatggcc ggtcagaaag tacaccaccc gatcctcggg attggtcagc ctgacgatgg
     5461 ccgaggtgat ctcgcgttcg ctggggaatt cgatgaggtg cgaatcttcg ccgagagtcg
     5521 ccgccatcga ggcatcccgg gtcactccaa atagatcggc agccagtgga ttggactgag
     5581 ggtcaatgaa ttcgtagctg accaacccat tgctcttgga aacaaactca tccagtagcg
     5641 gccggatctg gtctcgcgaa ctcgcccggg ctggagtata gaagccggtc agtttcaccg
     5701 gctcggagag ctcggagagc aacagctcag tctcggggga aagcgagaac tccttgtcct
     5761 cggtcaggtc gatccgc
//
LOCUS       DATDPQ010000056         3579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_21030, whole genome shotgun sequence.
ACCESSION   DATDPQ010000056 DATDPQ010000000
VERSION     DATDPQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21030"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..743
                     /locus_tag="VI520_01260"
     CDS             201..743
                     /locus_tag="VI520_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36541.1"
                     /translation="MKIRPRDAGVAGMILGGLLLAACTARAELSPSTPTPAAPTSLPT
                     AVGPEVQPGTWAVSFSYEFPAGFWLQGDHRYGFYMDCPVLQQFNVPSEYRDFTVTLDT
                     IAFESPVYLRLGGLSTGALAPINLDAIHPDQATVAVVTLLGITEDQAQQAGQTSECEI
                     VIGWDAMNAQTLIAAEPFRP"
     gene            complement(780..1637)
                     /locus_tag="VI520_01265"
     CDS             complement(780..1637)
                     /locus_tag="VI520_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF36542.1"
                     /translation="MRVKSIGRIGARLLTGAALVAACGATEPETQPPPSPETGIQLEP
                     QLPTGVQLQASFAVPGMLDGQPTIQEEMRYLLYLPSGYGQTPDTLWPLILFLHGSGDD
                     DYDSAFVLSYGLPAVLQAGEQPDEFPFIVVSPQASPGGTWWTEDALPVLDALLGEVLE
                     TYRVDPDRVYLTGLSMGGYGAWYLATTYPERFAAMISVSGSGYRTPFPPAEETLCRME
                     DLAVWAIHGAQDQMSDPEASRLNTLALKASCLGEVQWTLYEDEGHFGTFERAYRDPQL
                     YLWLLEHHR"
     gene            complement(1705..2283)
                     /locus_tag="VI520_01270"
     CDS             complement(1705..2283)
                     /locus_tag="VI520_01270"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF36543.1"
                     /translation="MAEAAFRLIVKTGPNPGTAFDLTKEVTLVGRDVTNDITVGDAEL
                     SRQHARLTRTPGGYVIEDLGSTNGSTVNGERLVAPRVLSPGDLLGMGQNVTLSFEATS
                     PEAARTVMAGAVRHGAAPSPEAIQARTPQPAAAAAPLPIEPAPRGGARRWVLAGAGCL
                     VLVGICAVTLFIMDTYFPDILYAPFRMLGFSG"
     gene            complement(2334..>3579)
                     /locus_tag="VI520_01275"
     CDS             complement(2334..>3579)
                     /locus_tag="VI520_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HLF36544.1"
                     /translation="AQSEREILVLSASGPLTPAALEYLDRGLQLAERRESEALILTLD
                     TPGGAVELMTRMIQSIRGSSVPVVIYIAPRGAIAGSAGTVITLAGHVAAMAPETAIGA
                     ASPVGGQGEDLGETIERKTKEILKAQARSLAARRGEAAVALAEATIEEAKAASAEEAL
                     QAGLIDIIAADLDDLLIQLDGFTVELESGPRTLRTTAAELFVVEQTFIEQLLGALTNP
                     NIVFLLMAIGVQSILIEISSPGGWVAGFIGVMSLALGAFGLGVLPVNWTGLIFLATAF
                     VLFILDIKAPTHGALTAAGVASFIAGALILFNSPGTPEFQRVSVPLVVVTGLAISAIF
                     LTIVTFALAAQRRRVEVGQETLIGRTGITKTELAPTGMVQVAGELWSAKVEDRSRTVP
                     AGEDVEVTGVEGLSLKVRARDG"
BASE COUNT          652 a         1202 c         1097 g          628 t
ORIGIN      
        1 gtcccggaga gccccagtaa agacgcatca tttctcttcc tcctctttct tgttcgctgt
       61 gtttgcgaat gagctgttgt aactttactc agggcccatc agaatgggat ttgtaccgcg
      121 taggattccc gttaaccctt ccgaccgatt cggagtcgat aagttagaat cctggtaagg
      181 agcgaggagc cgcgccgaga atgaaaattc gaccgcggga cgcgggggta gccggcatga
      241 tcctcggggg cctgctgctg gcagcctgta cggcgcgggc cgagctttcg ccgtcgaccc
      301 cgaccccggc cgctccgacc agcttaccga ccgcggtggg acccgaagtt caaccgggaa
      361 cctgggcagt ctcttttagc tacgaattcc cggccggctt ctggctccaa ggcgaccacc
      421 gatacggctt ctacatggac tgcccggtgc tgcagcagtt caacgttccc agcgagtatc
      481 gcgatttcac ggttactctg gatacgatcg ccttcgagtc gccggtctat cttcggctgg
      541 gcggcctgag caccggcgcc ctggccccga ttaacctgga tgcaatccat cccgatcagg
      601 ccacggtagc ggtcgtcacg ctgctcggga tcactgaaga ccaggcccag caggccggcc
      661 agacgagcga gtgcgagatc gtgatcggct gggacgcgat gaacgcccag accctgatcg
      721 cagccgaacc gtttcggccc tagcccgggc gccccagctc acttcgactc acccggcctt
      781 cagcggtggt gttccagcag ccacaggtag agctgcgggt cccgataggc ccgttcgaac
      841 gtgccgaagt ggccttcatc ttcgtaaagc gtccactgaa cctcgcccag acaggaggcc
      901 ttcagggcca gcgtgttgag gcgcgaggcc tccggatcag acatttgatc ctgcgcgcca
      961 tgaatggccc agactgccag atcctccatc cggcagaggg tctcctctgc aggcgggaac
     1021 ggagtgcggt atccgcttcc ggaaaccgag atcatggccg caaatcgttc ggggtaggtg
     1081 gtggccaggt accaggcgcc gtagcccccc atgctcagac cggtgagata cacccggtca
     1141 ggatccactc ggtacgtctc gagcacttct ccgagcaagg cgtccaggac gggcagcgca
     1201 tcctcggtcc accatgtccc accgggggaa gcttgcggag ataccacgat gaacgggaac
     1261 tcgtccggct gctcgccggc ctgcaagact gccggcagac cgtagctcag cacgaaggcc
     1321 gagtcatagt catcatcgcc ggatccgtgc agaaacagga tcagcggcca gagagtgtcg
     1381 ggggtctggc catatccgct cggcaggtag agcagatagc gcatttcctc ctggatcgtc
     1441 ggctgcccat ccagcatacc cgggaccgcg aagctggctt ggagttgaac ccccgtcggc
     1501 agctgtggct ccagctggat cccggtctcc ggcgagggcg gcggctgagt ctcgggctcg
     1561 gttgccccgc aggcagccac cagcgcagca cccgtcagca aacgggcacc gattctccca
     1621 atcgatttca ccctcataga ttgttgaatt gaagagcatg ggcagccgac ggccgcccac
     1681 ccgcacaggt ctggccgagc agcctcagcc ggaaaacccc agcatcctga atggcgcgta
     1741 gagaatgtcg gggaagtagg tatccatgat gaagagagta actgcgcaga ttccgaccaa
     1801 caccagacag ccggctccgg caagcaccca gcgcctcgcg ccgccgcggg gggccggctc
     1861 gatcggcagc ggcgccgcgg cagcagcggg ttgaggggtc cgggcctgga tcgcctcggg
     1921 cgagggcgcc gcgccgtgcc ggactgcccc agccatcacc gttcgagccg cttcgggcga
     1981 ggtcgcctca aaactcaggg tcacattctg gcccatcccc agcaaatccc ctggtgagag
     2041 caccctcggg gcaaccagcc gttctccgtt gacggtgctc ccgttcgtgg agcccaggtc
     2101 ttcaatgacg tagccccctg gggttcgggt gagccgggcg tgctggcgag agagctccgc
     2161 atccccgacg gtgatgtcgt tggttacgtc tctgccgacc agggtgactt ccttggtcaa
     2221 gtcaaaggct gtcccagggt tggggccggt cttgacgatc aaacggaagg cagcttcagc
     2281 catggcctcc ctctcctttg ggaacggtag tgtacaggcc ctcgaaactc acgtcaaccg
     2341 tcacgcgccc ggaccttgag actcaggccc tccactccgg tcacctctac atcttcccct
     2401 gccggcacag tccgggaacg gtcctcgacc ttcgccgacc acaattcacc ggccacctgg
     2461 accattccgg tcggagccag ctcggtcttt gtgattccgg tccgcccgat cagcgtctct
     2521 tggccgacct ccactcgccg gcgttgggcc gccagtgcga aggtcacgat ggtcaagaag
     2581 atggccgaga tggcgagccc ggtcacaacg accaacggga ccgacacccg ctgaaattcc
     2641 ggcgtccccg gcgagttgaa caggatcagc gccccggcaa tgaaagaggc caccccggcc
     2701 gcggtgagcg ctccgtgggt cggagccttg atgtccagga tgaacagcac aaaggcggtg
     2761 gcgaggaaga tcagcccggt ccagttcacc ggcagcacgc caagcccgaa ggcccccagg
     2821 gccagcgaca tcactccaat gaagcccgcc acccatccgc cggggctgga aatctcgatc
     2881 aagatggact gcactccgat cgccatcagc aggaaaacaa tgttggggtt ggtcagggcc
     2941 cccagcagct gctcaatgaa ggtctgctcg accacgaaca actcggcggc cgtggtgcgt
     3001 agcgtacgcg ggccgctctc cagttcgacc gtgaaaccat ccagttggat tagcaggtca
     3061 tccagatcgg cggcaatgat gtcaatcagt ccggcttgca acgcctcctc ggccgaggct
     3121 gctttagcct cctcaatggt cgcctccgcc agcgcgaccg ccgcctcacc ccgtcgggcg
     3181 gccagactgc gggcctgcgc ctttaggatt tccttagtct ttctttcgat cgtttccccc
     3241 agatcttctc cctggcctcc gaccgggctg gccgcgccga tggcggtctc gggagccatg
     3301 gcggccacgt gaccggcgag cgtaattacg gtgcccgcgc ttcccgcgat ggctccccgc
     3361 ggggcgatgt agatcacaac cggcaccgag cttccccgga tggactggat catccgggtc
     3421 atgagctcga ccgccccacc gggggtgtcc agggtcagga tcagggcttc ggactctcgc
     3481 cgctccgcca gctgcaggcc ccgatcgagg tactccagcg cggcaggcgt gagcggccca
     3541 ctcgcgctca gcaccaggat ctcccgctcg ctttgggct
//
LOCUS       DATDPQ010000057         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_281105, whole genome shotgun sequence.
ACCESSION   DATDPQ010000057 DATDPQ010000000
VERSION     DATDPQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281105"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VI520_01280"
     CDS             complement(<1..234)
                     /locus_tag="VI520_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36545.1"
                     /translation="MKSRRARPGRWLPALSALIALAGWEGVARASGLPQFILPPPSAV
                     AARLSRALLDGSLLGHTLVTLTEVLAGLALGVSV"
     regulatory      complement(426..555)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            714..2264
                     /locus_tag="VI520_01285"
     CDS             714..2264
                     /locus_tag="VI520_01285"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HLF36546.1"
                     /translation="MKTSQLATAEGVLFTDQYQLAMAQVYFRHGLASKLALFDYFFRD
                     YPNYDGHQAGFCVNAGLEWLLDWMAEAHFRKQDLEHLQGQRSAVGQPLFAQDFLEWLG
                     QEGSFHGFSLRAIPEGRVVHPRVPLVQVSGPLALAQILETPLLNHLNYQTLVATKAAR
                     IKDSARGGTVLEFGLRRAQGKGANAGARAALIGGADFSSNVGISHLLGFPPKGTHAHS
                     MVQVFLALGMDELDAFRAFASIYPDDCILLVDTIDTLESGIPNAIRVFEALRAEGHEP
                     LGVRLDSGDLAYLSIQAAKMLDAAGFPAARIVLSNNLDELVIWQITTQVSREAGRYGV
                     DPDSLIRRLVYGVGTRLITSWGEPALGGVYKLVAIEDGSEWLPALKLSETADKIPIPG
                     PKKAWRIYDQRGMATVDLLCLAEEEPANLDPVRTFHPVDPALQRTLPRAAVKEIEPLL
                     IEVMHAGRLVGQSPKIEEIRQLRDADLARLDPGVRRLINPHVYHVSLSERLQVLKQSL
                     IETTGSRS"
     gene            complement(2285..3220)
                     /locus_tag="VI520_01290"
     CDS             complement(2285..3220)
                     /locus_tag="VI520_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HLF36547.1"
                     /translation="MKPGVSFPDFYDAERVGALYAPDTAAAIRAGNGLDLPPAEQDRP
                     RVALMLVDAQVDFIHRDGTLSVPGAVADTRRTVEWIFSNISWLTQIAASLDSHVPNQI
                     FYPTWWIGNDGNPPAPFTPISAEQVRSGIWRPLFEVEWSKNYVETLERQAKKTLMIWP
                     YHTMIGSPGHAISPALYEAIAYHSAARQSQPRFVTKGTVAKTEHYSLFEPEVPIPDVP
                     DAALNRAFIDWLLGYDLVYVAGQAKSHCVLESMNSLVRQFADQPQVVGKIRVLMDCTS
                     SVQHPEIDFEAIAETAFREFAKRGVQLVKSTDPIG"
     gene            complement(3269..>3529)
                     /locus_tag="VI520_01295"
     CDS             complement(3269..>3529)
                     /locus_tag="VI520_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HLF36548.1"
                     /translation="QSLAAYAGRDAAKARAVADLDDQMDGLYKAVFSELVEIMSHTPD
                     SAARATYLLWCAHNLERIGDRVTNIAERVVFVTTGDLKELND"
BASE COUNT          666 a         1095 c         1077 g          691 t
ORIGIN      
        1 cacgctcacc cccagcgcca gtccggctaa aacttcagtc aaggtcacca gcgtatggcc
       61 cagcaggctg ccatccagca gggctcggct cagccgggcg gccaccgccg acggcggcgg
      121 gaggatgaat tgcggcagcc cgctggcccg ggcaacccct tcccagccgg ccagcgcgat
      181 cagggccgag agggccggca gccagcgccc cggtcgggcg cgcctgctct tcatcccttg
      241 aatggcttgc atcgctttgc tcttaagcaa acctgccccg agatcgatcg ctcggggcag
      301 gcaaatcggc tgctgcagtc ctcagaaggc aatggcctcc atcggatctt ccagattagc
      361 cttgtcaaga agccgccccg tcgacgtagt gtccgcgggg cagcatcaat gctcaggcta
      421 atgcctgaag cctcctccta tccggactgt accgtcggcc ccggagttgc accggatcct
      481 gcgctctcgc gctcgcgggc tataccgccg atcgggaatt gggcttgtgg ccctcaccct
      541 gccccgaagg cttctcgaac tattcggttg tgggccggat tatacctgtt tgccgcgaaa
      601 gcgcggaaga ggtcggctca cattccttta gatctgggta taacctgggt tgagacgaga
      661 gcgtcctgga gctagcatcg ccgtccagtg acgccaagcc aggagacgtg gccttgaaaa
      721 ctagtcaatt agcaacagcc gagggggtcc tattcacgga tcagtaccag ctcgccatgg
      781 cccaagtcta cttccggcac ggattggcat cgaaactcgc cctgttcgat tacttcttcc
      841 gtgactatcc aaactacgac ggccaccagg ctgggttctg tgtcaacgcc ggattggagt
      901 ggctgctcga ttggatggcg gaagcgcact tccgcaagca ggatctcgag cacctccaag
      961 gacagcgcag tgcagtggga cagccccttt ttgcacagga cttccttgaa tggctagggc
     1021 aggagggcag cttccatggc ttctctctgc gggcgatccc tgaagggagg gtcgtccatc
     1081 ccagagttcc cttggttcaa gtgagtggac ccctggccct ggcgcagatc ctggagaccc
     1141 ctctcctgaa tcatctcaac tatcagaccc tcgtggcgac caaggcagcc cgcatcaaag
     1201 acagtgcccg gggaggcacg gtcctcgagt tcggtttgcg gcgggcccag ggcaaggggg
     1261 ccaacgccgg agcgcgggcc gcgctgatcg ggggcgcaga cttcagctca aatgtcggca
     1321 tctcccacct gctcggcttt ccgccgaaag ggacccacgc ccacagcatg gtgcaggttt
     1381 tcctggcgct cggaatggat gagttggacg cgttccgcgc gttcgcctct atttatccgg
     1441 acgactgcat cttgctggtg gacaccatcg acaccctgga gagcggaatc cccaacgcca
     1501 tccgagtttt cgaggcgctc cgggccgagg gacatgagcc gcttggcgtt cgcctggatt
     1561 ctggcgacct ggcttacttg agcatccagg ctgccaagat gctggatgcg gccggctttc
     1621 cagctgccag gatcgtgtta tcaaacaacc tcgacgagct ggtgatttgg cagatcacga
     1681 cccaggtctc ccgagaggcc ggccgctatg gtgtggatcc ggactcgttg atccgcagat
     1741 tggtgtacgg agttggaact cggttaatta cttcctgggg ggaaccggcg ctgggcggcg
     1801 tctataaact ggtcgccatc gaggatggct ccgaatggct gccggcgctc aaactatccg
     1861 agaccgccga caagatcccc ataccagggc cgaagaaggc ctggcggatc tatgaccagc
     1921 gcggcatggc tacggtggat ctgctttgcc tggctgagga agagcctgcg aatctggatc
     1981 cggtgcggac cttccatccc gtagaccccg ctctgcagcg aacgctgccc agagcggcgg
     2041 tcaaggagat cgaacccctc ttgatcgagg tgatgcacgc gggcaggctc gtggggcaga
     2101 gtccgaagat cgaagagatc aggcagctac gggacgcaga tctggcacgc ctggatccgg
     2161 gggttcgacg cctgatcaac ccccacgttt accatgtgtc gctcagcgag cgcctgcagg
     2221 tccttaagca atcgctgatc gaaacgaccg gatcccgctc ctgatccaaa gcggccgcgc
     2281 agttctatcc gatcggatcc gtcgatttca ccagctggac cccgcgcttg gcgaactccc
     2341 gaaacgccgt ctccgcgatc gcctcgaagt cgatttctgg atgctggacc gaggaggtgc
     2401 agtccatcag cacccggatt ttccctacca cctgtggctg atcggcgaac tgacgcacca
     2461 gcgaattcat cgattccagg acgcagtggc ttttcgcctg gcctgcgaca tagacgagat
     2521 cgtagcctag cagccaatcg atgaatgcgc ggttgagcgc ggcatctggc acatccggga
     2581 tcgggacctc cggctcaaac agcgagtagt gctcggtctt tgcgaccgta cctttggtca
     2641 caaacctcgg ctgggattgg cgggccgcgc tgtggtaggc gatcgcttca tacagcgctg
     2701 gtgagattgc gtgtccgggt gatccgatca tggtgtggta gggccagatc atcagtgtct
     2761 tcttggcctg gcgctccaac gtctcgacgt agttcttcga ccactcgact tcgaaaagcg
     2821 ggcgccaaat ccccgatcgg acctgctccg ccgagatcgg cgtaaagggg gcaggcgggt
     2881 tgccatcatt cccgatccac caggtcgggt aaaagatttg attagggacg tgagagtcca
     2941 gcgaggcggc gatctgtgtc aaccagctga tattcgagaa gatccactcg accgtgcgcc
     3001 gggtgtctgc aaccgccccg ggcacgctca gagttccgtc gcgatggatg aagtcgacct
     3061 gcgcatcgac caacatgagc gcgacccgcg gtcggtcctg ctcggccggc ggcaggtcta
     3121 acccgtttcc tgcccggatc gctgcagcgg tatcgggtgc atacagcgca cctacccgtt
     3181 ctgcatcgta gaagtcgggg aagctgacgc cgggtttcat ccggagcaac cttcgcagtc
     3241 ggtggcagtg atactcagcg gcaccgcctc agtcgttaag ctcctttaga tcgcccgtgg
     3301 tcacgaacac cactcgctcg gcgatgttgg tcactcgatc tccgatacgc tcgaggttat
     3361 gagcgcacca gagcaggtag gtggctcggg ccgcgctgtc gggcgtatga gacatgattt
     3421 cgaccaactc actgaagacg gctttataga gtccgtccat ctggtcatcc aggtcagcga
     3481 ccgcccgggc cttagccgca tctcggccag cataggcggc caagctttg
//
LOCUS       DATDPQ010000058         6979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2372098, whole genome shotgun sequence.
ACCESSION   DATDPQ010000058 DATDPQ010000000
VERSION     DATDPQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372098"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VI520_01300"
     CDS             complement(<1..406)
                     /locus_tag="VI520_01300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HLF36549.1"
                     /translation="MSYIPHTELERRQMMSAVGVESIDELFADVPAAVRFPDLDLPEP
                     ASELEILEELQLLAEANLNTREAACFLGAGAYNHYIPSVVDHILRRNEFYTAYTPYQP
                     EISQGTLQAIFEYQSLMCFLTGMEVSNASHYDG"
     gene            complement(406..798)
                     /gene="gcvH"
                     /locus_tag="VI520_01305"
     CDS             complement(406..798)
                     /gene="gcvH"
                     /locus_tag="VI520_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLF36550.1"
                     /translation="MNYPEDLKYAGTDEWIRVEDDLATLGVSDFAQDQLSDVVFFEAT
                     VKVGERVAAGAQVATVESVKAAADVSSPVSGEILEINQPLASNPELVNTDPYGSAWMI
                     KLKLTDKSELAALMDHIGYQRNTEKRGS"
     regulatory      complement(928..1034)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1187..2197
                     /locus_tag="VI520_01310"
     CDS             1187..2197
                     /locus_tag="VI520_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF36551.1"
                     /translation="MADELTLITGAAGEIGHGLLDSLGSATADRLLALDRDPLELDLR
                     DRCREFIQGDVLDEALLADLGKRYRIGTIYHLAAVLSTRAEQNPRAAHRVNVEGTFNL
                     LQLAVDQTQRRGEPVTFVFPSSVAVYGFPTLAAKLASGPQTEQARCSPTTIYGASKLY
                     CEHLGAYFSQSQPRGPGQVGIDFRGLRLPGLISADTLPTGGTSDYGPEMLHHAAEGRL
                     YRCFVRPDTRMPFMTMPDAVRALIRLAEAPSLRRQVYNVTGFSPSAEELQRIVMEFFP
                     NAEITYEPDARRQAIVDSWPAEVDDRAAREDWGWAPQHGLRTAFTEYLIPAVSKRYAP
                     AH"
     gene            complement(2145..3302)
                     /gene="alr"
                     /locus_tag="VI520_01315"
     CDS             complement(2145..3302)
                     /gene="alr"
                     /locus_tag="VI520_01315"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLF36552.1"
                     /translation="MTDPRNLYSNWVEVDLAVIEANTRRLTTLTKAAVMAVVKANAYG
                     HGAIPVAQAAARGGADWFGVARAEEALDLRAAGIGLPILVLGPAPTDRLPALIAAGVS
                     LTVSDEVQIDAAARAARDQQATAKIQLKLDSGMNRLGAAPEAAVSLARRMGQHPGLSF
                     EGAFTHFARADEGVPQPVHRQLEQFEHALSALSSAGVRPPLIHAANSAATLGWPETHF
                     DLVRVGIALYGLRPSPAAPLPAGLQPALEWKTQLTRIRSVPPGEGISYGHEYVTRTTE
                     RIGTVLVGYADGLRRVAGNQLLVGGVNVPMVGRVCMDFCMVQLDRVPGAKEGDEAVII
                     GTQGETRLSAEQVADRWGTINYEVTCGIAARVPRLNVPERTVWRPPGSGTQ"
     gene            3385..4503
                     /gene="tgt"
                     /locus_tag="VI520_01320"
     CDS             3385..4503
                     /gene="tgt"
                     /locus_tag="VI520_01320"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLF36553.1"
                     /translation="MTEFSFEIIAQDGRGRAGLFHTPHGTLETPLFAPVGTQATVKAL
                     TPVQLRELGASLVLANAYHLYLRPGDTQIAQLGGLHKFMDWSGPLLTDSGGYQLFSLA
                     AQREVDADGVTFRSHLDGTPHRITPETAMGIQENLGADIVMTLDECAEPYDREYNRQA
                     LERTHAWARRCLEAKTRADQALFGIVQGGIFPDLRRESAEFVASLDTPGIAIGGLSVG
                     ETKNEMLATLELLDAALPTDRPRYLMGVGAPGDLLEAIPRGVDLFDSVLPTRLARNDA
                     ALTRHGRVNLRWATLADDPGPLDPDCSCYTCTHFSRAYLRHLVVGREMLSATLLSIHN
                     LHLLIDLTQELRQAIIDGRLASTIESLREGLASGERPE"
     gene            4500..5345
                     /locus_tag="VI520_01325"
     CDS             4500..5345
                     /locus_tag="VI520_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLF36554.1"
                     /translation="MTWYQALILGMVQGASEFLPISSSAHLVLVPWLLGWQFEPIAFF
                     IFGVTVHWGTLAAVVLAFRDDLLRFGRAALLAVLHRDLLETPEATLAWLLLLATLPGA
                     LAGVVLGGMVEQAFSNPPAVAGLLLGTAVLLALGERSRSIRAANPDEFRPLKSLRIRD
                     AILIGLAQAVALLPGISRSGATISAGLFRGLDRGDAVRFSFLMSVPIIFGAGLVALAE
                     LAGEPGAAQAVPSLVIGLLAAGVVGFIAIRWLLRYLEGGTLRGFAIYCALAGAAGLLV
                     SLIRG"
     gene            5338..6120
                     /locus_tag="VI520_01330"
     CDS             5338..6120
                     /locus_tag="VI520_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLF36555.1"
                     /translation="MARTALILSLSIVLSGCGQLPPGARGTATIPVPTPFPEIATTPS
                     FQAWTTNRILAFREQKGSAAQRSYIGLGLQILAPAATLPAVEAGEFELVVSAQEPPAD
                     WFATPLGWEPIVFAVHSGNPVHDLSLVQLASIFRGQASNWSLFDGPDLGITPIIPLRG
                     DELREALAGVLLGNSRYSTQALLGPSPEATLALVGQTPGAIGILPLSAVVEGVLVLQV
                     GGLAPAVGDERYPLQVEVLGFAPQEPSGVVREWLAWLQAEGS"
     gene            complement(6124..6798)
                     /locus_tag="VI520_01335"
     CDS             complement(6124..6798)
                     /locus_tag="VI520_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF36556.1"
                     /translation="MTQAQYRIAGLDIVAFSLGPAQTNAYLVGDPKAGVAVVVDPAWD
                     GELIQAEAARRDWRVTCIWLTHAHFDHFGGAAALSDASETPIPVALHAEDHPLWRMRG
                     GASAFGFPDFDPGPEPTVALEHGMQMQLGGSGFEVRHTPGHTPGHVVFRELAAGLVFC
                     GDLIFQASVGRTDLPGGDQAVLLGSIRDEILSLPDTTRLFPGHGSPTTVGEERQSNPF
                     LRGLAG"
BASE COUNT         1187 a         2285 c         2205 g         1302 t
ORIGIN      
        1 caccgtcgta atgggaagcg ttcgagacct ccatcccggt taggaaacac atcaaactct
       61 ggtactcgaa gattgcctgc agagtcccct gggagatctc gggctgatag ggtgtgtagg
      121 cggtgtagaa ctcgttgcgc cgcaggatgt ggtcgacgac ggacgggatg tagtggttgt
      181 atgcgccggc cccaaggaaa caggccgcct cacgggtgtt cagattcgcc tccgccagca
      241 actgcagctc ctccaagatt tcaagctcgg aggctggttc ggggagatct aggtccggga
      301 agcggaccgc ggctggaacg tcggcaaaca actcatcgat cgattcgaca cccaccgccg
      361 acatcatctg gcggcgctcc aactcggtgt gcggaatgta gctcatcaac tgccccgctt
      421 ttcggtgttg cgctgatagc caatgtggtc catcagggcc gccagctcgg acttgtcggt
      481 taacttgagc ttgatcatcc aggcgctccc atacgggtcg gtgttgacca gctccggatt
      541 ggaggcgagc ggctggttga tctcgaggat ctcgcccgag acgggcgagc tcacgtcggc
      601 cgcggctttg accgactcca cagttgccac ctgcgctccg gccgcgaccc gctcgccgac
      661 cttcaccgtg gcttcaaaga acaccacgtc cgagagttga tcctgggcaa agtccgacac
      721 ccccagggtg gccaggtcgt cttccacccg aatccactcg tccgtgccgg catacttcag
      781 atcctcggga tagttcatcc tgacctcctc aatcgcccaa ccggccgaga acaaaaaagg
      841 gcacctcgtt gagtgccctc tgtctgggac ctgagagatt cgccttgcgg cttgcccctt
      901 cggtgcgccc ggcgctttcc agaggtgcgg ccaggtgggg tccttttacc tgagagattc
      961 gcccgccgcc acgactgacg gtcgaacttg ccccttcggt gcccgacgct ccgggtctct
     1021 tccccgcctg tgcagggttt tattctatgg acgccaacca ccaaagtcaa gctgaagtcc
     1081 gcagtttagt tccggcggaa catcggcggg accttgattc tttccggtgt actgtgccaa
     1141 gataccgcag ggtgaactcc tcgcgggcga aaactcccag ccaagtatgg ccgacgagct
     1201 gactctgatc accggcgcgg ccggtgaaat cgggcacgga ctgcttgact ccttggggtc
     1261 ggccacggcc gatcgattgt tggcactcga tcgcgatccg ctcgaactgg acctgcggga
     1321 ccgctgccgc gaattcattc agggagacgt gctcgacgag gcactgctgg cggacctggg
     1381 gaagcgctac cgaatcggga cgatctacca tctggcggcg gtgctttcca cccgggccga
     1441 gcaaaacccc agagccgccc accgggtgaa cgttgaaggg acattcaatc tgctgcagct
     1501 tgcggtcgac cagacccagc ggcgtggcga accagtgact ttcgttttcc ccagcagcgt
     1561 cgcggtgtac ggctttccga ctctggcagc caagctggca tccggacccc agaccgagca
     1621 ggcccggtgc tccccgacca cgatctatgg cgccagcaag ctctactgtg agcacctggg
     1681 tgcgtacttc agccagtcgc agccccgcgg tccgggccag gttgggatcg atttccgtgg
     1741 tctgcgctta ccggggttga tcagcgcgga caccctgccg accggcggga ctagcgacta
     1801 tgggcccgag atgctgcacc acgccgccga aggccggctg taccgctgtt tcgtgcggcc
     1861 cgacacccga atgcccttca tgacgatgcc ggatgccgtg cgggccttga tccggctggc
     1921 cgaggcgcct tcccttcggc ggcaggtcta caacgtcacc ggattcagcc cttcggccga
     1981 ggagctgcaa aggatcgtaa tggaattttt ccccaacgct gaaattacct atgagccgga
     2041 cgctcgccgg caggccatcg tggacagctg gccggcagag gtggatgatc gcgccgcgcg
     2101 ggaagactgg ggctgggcgc cgcaacatgg actgcggacc gccttcactg agtacctgat
     2161 cccggcggtc tccaaacggt acgctccggc acattgagcc gcggcaccct cgctgcgatc
     2221 ccacaggtca cttcataatt gatcgtgccc caacggtcgg caacctgctc ggccgacagg
     2281 cgggtttcgc cctgggtgcc gatgatcacc gcctcatcgc cttccttggc gccgggaact
     2341 cgatcgagct gcaccataca gaaatccatg cacacccggc ccaccattgg cacattgacg
     2401 cccccgacca gcagctgatt ccccgcgact cgccgcaggc cgtccgcgta accaaccagg
     2461 acggtcccta tccgttcggt cgtgcgagtc acgtactcat ggccgtagct gatcccttcc
     2521 ccgggaggca cggatcggat ccgagtcagc tgggtcttcc actccaaggc cggctgcaac
     2581 ccggccggca gcggggcagc cggggaggga cgaagaccgt agagtgcaat tcccacccgg
     2641 accaggtcga agtgggtctc cggccaaccc agggtggcgg ccgaattggc agcgtggatc
     2701 agcggaggcc gcacacccgc cgagctgagt gctgagaggg catgctcgaa ctgctccagc
     2761 tgccgatgga ccggctgcgg cactccctca tctgcccggg cgaaatgggt aaacgcgccc
     2821 tcgaaactga gtcccgggtg ctgccccatc cggcgagcca ggctgaccgc ggcctctggc
     2881 gcggccccga gccggttcat tccggagtcg agtttgagct ggatcttggc cgtcgcttgt
     2941 tgatctcggg cggcccgcgc cgcggcatcg atctgaacct cgtcgctcac ggtgagcgag
     3001 accccggccg caatgagggc gggcagccgg tctgtcggcg cgggacccag gacgaggatg
     3061 ggaaggccaa tcccggccgc ccggaggtct agggcctctt cggcgcgagc aactccaaac
     3121 cagtctgcgc cgccgcgggc ggcagcctgg gccaccggaa tcgccccatg gccgtaagca
     3181 ttggccttga caaccgccat cacagcggct ttggtgaggg tggtcaatcg gcgggtgttg
     3241 gcttcgatga ctgccaggtc aacttcgacc cagttcgagt acagattccg gggatcggtc
     3301 acggccgaag tatacggagc gacagcacgg ctgtctacca caggtataat cgctcgctcg
     3361 cgcggcccgg cttcccccga agtaatgact gagttttcgt ttgagatcat tgcccaagat
     3421 ggtcggggcc gggcgggcct gttccacacc ccgcacggaa ctctcgagac cccgctcttt
     3481 gctcccgtgg gaactcaggc cacggtcaag gcgctcaccc ccgtccagct gcgcgaactc
     3541 ggggccagct tggtgttggc caatgcctac catctgtact tgcggcctgg cgacacacag
     3601 attgcgcagc ttggagggct gcacaagttt atggattggt ccggtccgct gttgaccgac
     3661 tccggtggat accaactctt ctcgctcgct gcccagcgcg aggtggatgc ggatggggtc
     3721 acatttcgtt cccacctgga cggaacccct caccgcatca ctcctgagac ggcaatgggc
     3781 atccaagaaa atctaggtgc cgatatcgtc atgaccctcg acgagtgcgc cgaaccgtac
     3841 gaccgggagt acaaccggca ggcactagag cgaactcacg cctgggcccg gcgctgcctc
     3901 gaagcgaaga cccgagccga ccaggccctt ttcgggatcg tacaaggcgg gatctttcca
     3961 gacctgcggc gcgagtcggc cgagttcgtt gcctcactgg atactcccgg catcgcgatc
     4021 ggcgggttga gcgtggggga aacgaagaat gagatgctgg cgaccctcga actgctcgac
     4081 gcggcccttc ccaccgaccg accccggtac ctgatgggtg tcggagctcc tggcgacctg
     4141 ctggaggcca tcccacgcgg agtagacttg ttcgacagcg tgctgccaac ccgcctggct
     4201 cgcaacgacg ccgccctcac ccggcacggc cgagtgaatc tccgctgggc gacgctggcc
     4261 gacgaccctg ggccgctgga tcccgactgc tcctgctaca cctgcaccca cttcagccga
     4321 gcctacctgc ggcatctggt tgtcgggcgg gaaatgctct ctgcgactct gctttcgatc
     4381 cacaacctgc atttgctgat tgacctgact caagaattgc ggcaagcgat catcgacgga
     4441 cgattggcca gcactatcga gagcctgcgc gagggattgg cgagtgggga gcgaccggaa
     4501 tgacctggta ccaggccctg atcctaggaa tggtccaggg agccagtgag ttcctgccta
     4561 tttcttcctc ggctcacctg gtcctcgttc cctggctgct cggctggcag ttcgagccaa
     4621 ttgcgttctt catcttcggt gtcaccgtgc attggggcac gctggctgcg gtagtcttgg
     4681 ccttccgcga cgacctgctc cgtttcggcc gagcagccct gctggcagtg ctgcaccgcg
     4741 acctgcttga gaccccggag gcgactctgg cttggctgct cttgctagcc acgctgccag
     4801 gggcactggc gggggtcgtc ctgggtggga tggttgagca ggccttctcc aatccgccgg
     4861 ccgtcgccgg cttgctgctg ggcaccgcgg tgctgttggc gcttggagag cgctcccggt
     4921 cgatccgggc cgcgaacccg gacgagttcc ggccgctgaa gagcctgcgg attcgagatg
     4981 cgatcctgat cgggctggct caggcggtgg cgctgctgcc cggaatctcg cgctccggcg
     5041 caacaatttc tgccgggctc ttccgcgggc tggaccgggg ggatgcggtc cgattctcct
     5101 ttctgatgtc ggttcccatc atcttcggag ccgggctggt ggcgctcgcc gagttggccg
     5161 gcgagcccgg cgcggcccag gctgttccat cactcgtgat cgggcttctg gcggccgggg
     5221 tggttggatt catcgccatc cgctggctgc tgcgctacct ggaagggggt acgctgcgag
     5281 gcttcgcgat ctattgcgcg ctcgccggcg cggctggcct gctggtgagc ctgatccgtg
     5341 gctagaaccg cgctgatcct cagcctttcg atcgtgctgt ccggatgtgg tcagttgccg
     5401 ccaggtgcgc gcgggaccgc gaccatcccg gtccccaccc ctttcccaga gatcgccacg
     5461 actcccagtt tccaggcttg gaccactaac cggatcctgg ctttcagaga gcagaaaggg
     5521 tcggcggctc agcggtccta catcgggctt ggcctgcaga tactggcccc ggccgccact
     5581 cttccggcgg tcgaagccgg agagttcgaa ctggtggtct cagctcagga gccgccggcc
     5641 gactggttcg cgaccccgct cggatgggag ccgatcgtat tcgctgtcca ctctggcaat
     5701 ccagtccacg atctctcact cgtccaattg gcgagcattt tccgcggcca ggccagcaac
     5761 tggagcttgt ttgacgggcc agatctcggc attacaccga tcatcccgct gcggggggat
     5821 gagctgcgcg aggccctggc gggggttctg ctggggaatt cgcgctacag tactcaagct
     5881 ctgttggggc caagcccgga ggcaaccctt gcgctagtcg ggcagacgcc aggggcaatt
     5941 ggaattcttc cgctctcggc ggtggttgaa ggggtcctcg tgctccaggt tggcgggctt
     6001 gctccggcag tcggggatga acggtatccg cttcaggttg aagtcctggg cttcgcaccc
     6061 caggaaccca gcggtgtcgt gcgggagtgg ttagcctggc tgcaggccga ggggagctag
     6121 cggctagccc gccagtccgc ggaggaaggg attggactgg cgctcttctc caaccgtggt
     6181 cggcgatccg tggccgggga acaggcgggt agtatccggc aggctcagaa tttcatcccg
     6241 aatgctgccg agcaagactg cctgatcacc acctggtaaa tcggtccggc caacgctcgc
     6301 ctgaaagatc aagtccccgc agaagaccag gccggcggcc agctctcgaa agaccacatg
     6361 ccccggcgta tggccaggtg tgtgccgcac ttcaaatccg ctgccaccca gctgcatctg
     6421 catcccatgc tcgagtgcga ccgtcggctc cgggccgggg tcaaaatcgg gaaagccaaa
     6481 tgccgacgcc ccaccccgca ttctccacaa tgggtggtct tcggcgtgca aggccaccgg
     6541 gatcggcgtc tcggaagcgt ccgagagggc cgcggcgcct ccgaagtggt caaagtgagc
     6601 atgggtgagc caaatgcagg tgacccgcca atcgcggcgg gccgcctcgg cctggatcaa
     6661 ttcaccgtcc caggcagggt cgacgaccac cgccaccccg gccttgggat ccccgaccag
     6721 ataagcattc gtctgcgccg gtccgaggct gaacgccacg atgtcgaggc cggcgatccg
     6781 gtactgggcc tgagtcaagc caggtccttt ccaaaggttc gagggaatga attcaacact
     6841 ggactaatgg tatcctgtcc cgcgtccctc gataacccgt gatgagcgtc gcagaattcg
     6901 tcgtccgcct cgcgcccgcg cgcgaccatc gcagcgccgc gcgctggatc ctctcgcacg
     6961 taaccgacca tcgcgggat
//
LOCUS       DATDPQ010000059         6267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_22343, whole genome shotgun sequence.
ACCESSION   DATDPQ010000059 DATDPQ010000000
VERSION     DATDPQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22343"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VI520_01340"
     CDS             <1..415
                     /locus_tag="VI520_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLF36557.1"
                     /translation="VIVFVVWVGIGPFAGALALGLHTVAALAKLFSEQVESILPGPLE
                     AIQSTGANRLQTIAFAVVPQVIPPYISFLMYRWDINVRMSTIIGFAGGGGIGFLLQQN
                     IGILNYRAASTQMLAIAIVVATMDYLSSTLRQRFV"
     gene            522..1025
                     /locus_tag="VI520_01345"
     CDS             522..1025
                     /locus_tag="VI520_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLF36558.1"
                     /translation="MNEPVLLLNANFEPLNVCTTRRAVGLLLYGKAEMLLNGRGHIRT
                     VRALYPRPSVIRLGYMVKRPYPRVRLSKREVFRRDDHACQYCGARATRLTLDHVVPRH
                     RGGEHGWTNLVTACSACNLKKGGRPLKDARMRLLRLPAEPQASAAYLFGSHLPANEDW
                     RQFLEGW"
     gene            complement(1044..1598)
                     /gene="raiA"
                     /locus_tag="VI520_01350"
     CDS             complement(1044..1598)
                     /gene="raiA"
                     /locus_tag="VI520_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLF36559.1"
                     /translation="MEVIINVRNLELTERLKEYVEKRVSRLDRYLDVLDEAIVDLRVV
                     ETARSAADRQIAQLTVRGKSVVLRAEDRADDIYPAVDSVLEKVERQIERYKGRRWKSR
                     GDGRSMGELLARDEQESDDAGWRIARRKRHQLQPMSELEAIEQMRLLEHEDFFIFLNE
                     AGNRVNLLYRRRDGALGLIESEIA"
     gene            complement(1648..2133)
                     /locus_tag="VI520_01355"
     CDS             complement(1648..2133)
                     /locus_tag="VI520_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HLF36560.1"
                     /translation="MPVRSYARYTGRLVQALLHLKYRPDRQLASVMADWLTGLYRPSE
                     WQASLVVPVPLSPGRFRQRGFNQAELLAHGLAKRLELPMAAAQLSRVRETRTQVGLDP
                     SERSLNVKGAFAAERGAFAGEAVLLVDDLYTTGATLAACAQAVLAEGGGPVWGLTVGR
                     A"
     gene            complement(2401..3351)
                     /locus_tag="VI520_01360"
     CDS             complement(2401..3351)
                     /locus_tag="VI520_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLF36561.1"
                     /translation="MSAALPIIAVVAVLVAIVVVIIGVRMPQSKDPIQERLSELMVSE
                     EAMTLEELELSQDFAQRIVVPFFNRIGQLAQRFTPQATLESAKKRLEMAGNPMQMNPA
                     FFLMLRFVFAFLFGTGLFLVFARTGRNLIQGIGISLLFMLIGFAFPNLWLTGRMRTRQ
                     KSIFRAMPDALDLLTISVEAGLGFDAAMAKVHEKWENELSLELGRVIQEIRLGKLRRD
                     ALRDMSERLGVNEMTSFVAAVIQSEQLGVSMAKVLRIQSDQMRVRRRQMAEEEAHKAP
                     IKMVFPIALLIFPSILIILLGPAAMLLLSSPLGGILGSGG"
     gene            complement(3367..4347)
                     /locus_tag="VI520_01365"
     CDS             complement(3367..4347)
                     /locus_tag="VI520_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLF36562.1"
                     /translation="MNPSAVLIVGGGLGLLLLLVGLVLTFVGGGSVVEERLGRYAETG
                     GLVTTGGEDAAVKGDRPSPLSDFFNRMGEGTDLFASIGVSLAQADIKLRPAEFIAATV
                     IGSVGAAGLALILGLPFLMAIPAALGGLFIPRIYVNQARRRRLKKFDDQLGDMLNLMV
                     NGLRAGFSTLQAMEAVSHEMPTPIADEFRRVVQEMQLGITTEEALDHMLRRIHSDDLD
                     LVITAINVQREVGGNLAEILDVISFTIRERVRIKGEIQTLTAQGRATAWVISALPIAL
                     MGMLYLVNRPYFMQFFNPETRNCGIPILCLALFMIGSGFYATQKIVDIDI"
     gene            complement(4353..5777)
                     /locus_tag="VI520_01370"
     CDS             complement(4353..5777)
                     /locus_tag="VI520_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HLF36563.1"
                     /translation="MSLLKRIEQGQTETSAPAEQKPFAPAPGGGGTSRLSGLTARRAA
                     PSGAGSGSRIIDIKNEVQNRLLAELDPSMDVTQTAEVRRQIEELFEGILVEMNIVLSR
                     PERRRLFEQIVAEILGLGPIEPLLSDDTITEVMVNGAKNIFVERAGKIHREPASFESD
                     DHLMRIIDRIVAPLGRRIDESSPYVDARLNDGSRVNAVIPPISLVGPVLTIRKFARNP
                     ISVEQLIESGTITAESIEFMKASVIARVNMVISGGTGSGKTTLLNVLSQYIPSDERIV
                     TIENAAELQLRQEHVVTLESRPPNIEGRGEVTIQNLVVNSLRMRPDRIIVGEIRDEAA
                     LDMLQAMNTGHEGSMTTAHSNSPRDTLSRIETMTMMAGMDLPTRAIREQIASALELVC
                     HLERMRDGTRKVTHITEIQGMEGDVITMTDLFVFEQTGLENGKVIGRLRPTGLRPKFM
                     EKIEQSGIHLPASIFGVGDRNLYR"
     gene            complement(5803..>6267)
                     /locus_tag="VI520_01375"
     CDS             complement(5803..>6267)
                     /locus_tag="VI520_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36564.1"
                     /translation="FLKRQFAYVIVDTASAMDDTTLAILDTTDLLIAIATPEIPAIKD
                     ARHLLDLLHILEFPTQNVCFVLNKMDRRSGISTEAVSENLKREVEAAIPLDERVVTAS
                     INKGVPLLLSDRSSPQARAMLQLMGTIKERLVKAEEPQTEDEQKERPRLFGR"
BASE COUNT         1232 a         1971 c         1821 g         1243 t
ORIGIN      
        1 ggtgatcgtg ttcgtggtct gggtggggat tgggcctttt gccggagcgc ttgcgctggg
       61 actgcatacc gtcgctgcac tggcaaaact gttctcggag caagtggaga gcatccttcc
      121 gggtccgctg gaagcgatcc aatccaccgg ggccaatcgg ctgcagacca ttgccttcgc
      181 cgtggttcca caggtcatcc ccccatacat ctccttcctc atgtaccgat gggacatcaa
      241 cgtgcggatg tcgacgatca tcggttttgc aggaggcggc gggatcggct ttttgttgca
      301 gcagaatatc gggatactca actaccgggc ggcgagcacc cagatgctgg cgattgcaat
      361 cgttgtcgcg accatggact acctcagctc gaccctacgt caacgctttg tatgacgtcg
      421 gccggcgttc acggagctat tcggcaattc gggctgccca gccgcaccag ttcgggaact
      481 gtcgcaactc ctcctggtga tataatccgc cggattcacc tatgaacgag ccggtgctgc
      541 ttttgaacgc caactttgag ccgctcaacg tgtgcactac ccgccgagct gtggggttat
      601 tgttgtacgg caaggcggag atgctgctca acgggagagg tcatatccgg acggtcaggg
      661 cgctctatcc caggccgtcc gtaattcggc ttggctacat ggtcaagcgt ccgtatcctc
      721 gagtccggct gagcaaacgg gaggttttcc gccgggacga ccatgcttgc caatactgcg
      781 gagcccgtgc cacccggctg accctggacc acgtcgtgcc tcggcaccgc ggtggcgagc
      841 acggctggac caatttggtg acggcctgct ccgcctgcaa tctaaagaag ggcggccggc
      901 cgctgaaaga tgcccgaatg cgattgctgc gactccccgc agaacctcaa gcctcggccg
      961 cctacttgtt cggatctcat ctgccggcaa atgaggattg gcgccaattc cttgagggtt
     1021 ggtgagccca aaaaaggcaa gccctaggcg atttccgact cgatcagccc cagcgcgccg
     1081 tctcgccggc ggtagagcag gttgacccga ttgccggcct cgttgagaaa gatgaagaag
     1141 tcctcgtgct ccagcaagcg catctgttcg atggcctcca gctcgctcat cggctgaagc
     1201 tggtgccgct tgcgccgagc gattctccag cccgcgtcgt ctgactcctg ctcatccctg
     1261 gcgagcaact ctcccatcga ccggccgtcc ccgcgcgatt tccagcggcg ccccttgtaa
     1321 cgctcgatct ggcgctcgac cttctccaga actgagtcca ctgccgggta gatgtcgtcg
     1381 gcccgatcct cggcgcgcag cacgacgctc ttaccgcgca cggtgagctg ggcaatctgc
     1441 cgatctgccg cgctgcgggc cgtctcgacc acccgcagat ccacgatcgc ttcatccagc
     1501 acatcaaggt atcgatccaa tctcgagacc cgtttctcga catactcctt caagcgctca
     1561 gtcagttcca gattgcggac attaatgatc acttccacgg aggtgcctcc ttgagcttcc
     1621 gacgtcgacc ggctgcggct gcctcggtca ggccctacct acggtgagtc cccagacggg
     1681 tccgcccccc tcagccagca cagcctgggc gcaagcggcg agggtcgccc cggtagtgta
     1741 caggtcgtcg accagcagga ctgcctcgcc ggcgaatgcc cctcgctcgg ctgcgaacgc
     1801 tcccttcaca ttcaagcttc gctcactcgg atcgagtccg acctgggttc gagtctccct
     1861 tacccggctg agctgggccg cggccatcgg aagctcgagc cgcttggcca gcccgtgggc
     1921 caatagctcg gcctgattga agccacgctg cctgaatcgg cctggactga gcggaactgg
     1981 gacgacgagt gaggcctgcc actccgaggg acgatacagc ccggtcagcc aatctgccat
     2041 aacggaagcc agctgccggt cgggacggta tttcaggtgc agcagggctt gaacgagccg
     2101 gccggtgtac cgggcatatg aacgtacggg caagacgggt ttgcgtctca ggcaggaggg
     2161 acaagcagac cagcctccaa gcgccaggcc gcaattgcca cagaacggtt cgctaaaccg
     2221 ttcgagagcg gctgcacaat tgctgcacca aaccgaaccc ggagcctggc agcccacaca
     2281 gtgaatcgga aataggagct cgagcccgct tccgagccac ctctggaacg cctggattgt
     2341 ttggcgcgtg ctgcgcttgg ggcctaactg gggtctctgc tcaggcagga cgccgaccac
     2401 ctatccaccc gacccgagga tgcctcccag cggtgaactg agcaagagca ttgcggccgg
     2461 ccccaggaga atgatcaaga tcgagggaaa gatcagcagc gcaattggaa agaccatctt
     2521 gattggggcc ttgtgagctt cttcctcggc catctgccgc cggcggaccc gcatttggtc
     2581 agattggatc cgcaagacct ttgccatact gacgcccaac tgctccgact ggataaccgc
     2641 cgcgacgaaa ctggtcatct cgttgactcc cagtcgttcc gacatgtccc gcagcgcgtc
     2701 gcggcgcagt ttccccaatc ggatctcctg aatcactctg ccgagttcga gcgaaagttc
     2761 gttttcccac ttctcatgta ctttcgccat ggcggcgtca aagcccaggc cggcctcgac
     2821 cgagatcgtg agcaagtcaa gcgcgtccgg catggctcga aagattgact tctgccgggt
     2881 tcgcatccgc ccggtcagcc aaaggttcgg aaaggcgaag ccgatcagca tgaagagcag
     2941 ggagattcca atgccttgaa tgaggtttcg cccggtcctg gcgaaaacca agaaaagtcc
     3001 ggtcccgaac aagaaggcga acacaaaccg gagcatgagg aagaaagccg ggttcatctg
     3061 catcggattg ccggccatct ctaagcgctt cttcgccgac tccagagtag cctggggcgt
     3121 aaagcgctgc gccagctgac cgatccggtt gaagaatggg acaacgatcc gttgggcgaa
     3181 gtcctgtgag agctcgagtt cctccagggt catcgcttct tcgctgacca tcagctcgga
     3241 cagccgttct tggatcggat ccttagactg cggcatccgc accccaatga tcacgaccac
     3301 gatggcgacc aagaccgcga caactgcgat tattggcaac gctgcagaca ttggactcac
     3361 cgcctcctaa atgtcaatat ccacgatctt ctgagtggca tagaagccac tgccgatcat
     3421 aaacaaagcg aggcaaagaa tcgggatccc acaattccga gtctcagggt tgaagaactg
     3481 catgaagtag ggccgattga ccaggtagag cattcccatc agcgcgatcg gcaaggccga
     3541 gatgacccag gcggttgccc gtccctgggc ggtgagggtc tggatctcgc ccttgatccg
     3601 cacccgttcc cgaattgtga atgagatgac atccaagatc tcggccagat tgccgccaac
     3661 ttccctttgg acgttaatcg ccgtgatcac taggtctagg tcatcactgt gaatccgccg
     3721 cagcatgtgg tcaagagctt cttcggtggt gatgcccagc tgcatctcct gcacgacccg
     3781 gcggaactcg tccgcaatgg gagtcggcat ttcgtggctc accgcctcca tcgcctggag
     3841 ggtcgaaaag cccgcccgca gcccgttgac catcaggttg agcatgtcac ccagctggtc
     3901 atcgaacttc ttgagccggc gccgtctggc ctggttgaca tagatccggg ggataaacag
     3961 gccgccaagc gccgccggga tcgccatcag aaacgggagc cccaagatca gggccagccc
     4021 ggcggctccg acggagccga tgacggttgc ggcgatgaat tccgccggcc gcagtttgat
     4081 gtcggcttga gcgagactga ccccgatcga ggcgaaaagg tcggtcccct cccccattcg
     4141 attgaagaaa tcggatagtg ggctcggccg gtcgcctttc acggcggcat cttccccgcc
     4201 cgtcgtgacc aggcctccgg tctccgcata gcggcccaat cgctcttcga ccaccgaccc
     4261 gccgccgaca aatgttagga ccaggcccac cagtaagagc aacaggccta gtccgccacc
     4321 gacgattagc accgcggagg ggttcatgga ttctatcggt agaggttgcg gtccccgacg
     4381 ccgaagattg aggccggcaa gtgaattccg gattgttcga tcttttccat aaacttcggc
     4441 cgcaacccgg tgggtcgcaa gcggccgatc accttgccat tctcgagccc ggtctgctcg
     4501 aagacgaaaa ggtcggtcat cgtgatgaca tcgccttcca tcccctggat ctccgtgatg
     4561 tgggtgactt tgcgggttcc atcccgcatg cgctccaggt gacacaccag ctcgagcgcc
     4621 gaagcgatct gctcccgaat ggcgcgggtc ggaaggtcca tgccggccat catggtcatg
     4681 gtctcgatcc gggaaagagt gtcgcgggga ctgttggagt gggcggtggt catggagccc
     4741 tcgtggccgg tgttcatcgc ctgcaacatg tcgagcgccg cctcatcccg gatctcccca
     4801 acgatgattc ggtcgggccg cattcggagc gaattaacca ccagattctg gatagtgacc
     4861 tcgcctcgcc cttcaatgtt cggtgggcga gattccagcg tgaccacgtg ctcctggcgc
     4921 agctgaagtt cggccgcgtt ctcgatcgtg acaatgcgtt catcggacgg gatgtactgg
     4981 gagagcacgt tgagcaacgt ggtcttcccg gagccggtgc cgcccgaaat gaccatgttc
     5041 accctggcga tgaccgacgc tttcatgaac tcgatcgact cggcagtgat tgtcccggat
     5101 tcgatcagct gctcgaccga gattggattg cgggcgaatt tccggatggt gagcaccgga
     5161 ccgaccagtg aaatcggcgg gatcaccgca ttcactcgag aaccgtcatt gaggcgggcg
     5221 tccacatagg ggctcgattc gtcaattcgg cggccaagcg gcgcaacgat tcgatcgatg
     5281 atccgcatca agtgatcgtc gctttcgaag gaggccggct cacggtggat cttccccgcc
     5341 cgctcgacaa agatgttctt tgccccgttg accattacct cggtgatggt gtcatccgag
     5401 agcaacggct caatcggacc aagcccaagg atctcggcca cgatctgttc gaagagccgt
     5461 ctccgctccg gtcgagagag cacgatgttc atctcgacca gaatgccctc gaagagttcc
     5521 tcgatctggc gccggacctc cgcggtctgg gtcacatcca tcgagggatc tagctcagcc
     5581 agcaaccggt tctgaacttc gttcttgata tcaataatcc ggctgccgga gccagcaccg
     5641 gatggagccg cccggcgggc agtcaggccg ctcagccgcg aggtaccacc accccccggg
     5701 gccggggcaa atggcttctg ctcggccgga gcactcgttt cagtttggcc ttgttctatc
     5761 cgcttaagta acgacaccga ctaaacctcc ggggctccgt ccctagcgac cgaacagccg
     5821 cggtcgctcc ttctgctcgt cttcagtttg cggttcctcc gccttcacca atcgttcctt
     5881 gattgtaccc ataagttgca gcatcgcccg cgcctgcggg ctgctgcgat cgctgagcaa
     5941 gagcggaacg cccttgttga tcgaagcggt gaccacccgt tcatccagcg gaattgccgc
     6001 ctcaacttcg cgtttcaggt tttcggagac cgcctcggtc gagatcccac tccggcggtc
     6061 catcttgttc aacacgaaac agacattctg ggtcggaaac tccaggatgt gaagcaggtc
     6121 cagcaaatgc cgcgcatcct tgatggccgg aatctcgggc gtcgcaattg caatcaacag
     6181 gtcggtcgta tccaggatcg ccagagttgt gtcatccatc gccgaggcag tgtcgacaat
     6241 cacgtaggcg aactgccgct tcaagaa
//
LOCUS       DATDPQ010000060         4670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1589790, whole genome shotgun sequence.
ACCESSION   DATDPQ010000060 DATDPQ010000000
VERSION     DATDPQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589790"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1983
                     /gene="dnaK"
                     /locus_tag="VI520_01380"
     CDS             112..1983
                     /gene="dnaK"
                     /locus_tag="VI520_01380"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLF36565.1"
                     /translation="MGRIIGIDLGTTNSVVAVMEGGEPTVITSSEGSRLIPSVVAINP
                     KTGERLVGQVARRQAIVNADNTIFSIKRFMGRKHSDPEVQRALDVVPYKVTGAANGDV
                     RVHMGGKEYAPPEVSAMILAKIKADAEAYIGEAVTQAVITVPAYFNDSQRNATKDAGK
                     IAGLEVLRIINEPTASSLAYGLDRKTDETIAVFDLGGGTFDISILDVGDGVFEVKSTN
                     GDTFLGGDDFDQRIIDWVVEEFRRDQGIDMRQDRQGLQRLREAAEKAKIELSTVAQTE
                     INLPFITADASGPKHLNLTLTRAKLEQLTEDLIERCVAPCNMALQDAELKAAQIDEVI
                     LVGGMTRMPAVQEAVRKIFEKEPHKGVNPDEVVAVGAAIQAGVLGGEVKDILLLDVTP
                     LSLSIETLGGVATALIERNTTIPARKTQVFSTAGENQTEVEIHVVQGERSMAADNKSL
                     GKFRLDGIPTAPRGVPQIEVTFDIDADGILNVSATDKATSRTQHITITASSGLSEVEV
                     EQMRTDAELHAGQDAERRDRAEARNVADNALYNARKVLDQNEGHIPQGARADLQAKLR
                     NLEQAMEEADTERLRKMSNELLAAVREVAARAQSGRAPGGENGSSSGDGVDREYFES"
     gene            2035..3150
                     /gene="dnaJ"
                     /locus_tag="VI520_01385"
     CDS             2035..3150
                     /gene="dnaJ"
                     /locus_tag="VI520_01385"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLF36566.1"
                     /translation="MADKRDYYEALGVGRDASADDLRSAYRRLAKQYHPDVNHEPGAE
                     DRFKEINEAYGVLSDDQRRAAYDRFGHAGLKGMPTDFDFGFTDIFEEFFGFGMSGRRR
                     RGAPRRGADLRYDLRLEFQEAVFGAEKEIEFVRRDICSSCRGTGAEPGTTPVRCPTCE
                     GSGEVRQVRQTFLGSMVNVTTCPNCGGRGELVSSPCRVCHSSGIEQQRVVRQIPIPAG
                     VDDGTQVRVTGEGEPGPNNGPRGDLYVVVQVLPHPYFRRRKDEILVDLSINIAQSALG
                     SEISVPTLDGEEKLRVPAGTQPGKVFRLKGKGVPHLRSNGRGDQLVIISVEVPKSLSP
                     EQRELFERLALTLGSEVKPQARSLLDDLKDLIGGLVD"
     gene            3156..4022
                     /locus_tag="VI520_01390"
     CDS             3156..4022
                     /locus_tag="VI520_01390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLF36567.1"
                     /translation="MEVSLRLRAELAEPAAELLGRLAPNGVAIEGLDDQGQAVLVRAW
                     LPVDGSLASRQAELEEGLWHLGQIQPFPSPIFRSVDEQDWAEIWKREYQPLPIGRRLM
                     VLPSWSTADPRGRLVVQLEPGMAFGTGAHPTTRHCLELIEETLRPGERVVDLGCGSGI
                     LSIAAARLGARRVLALDTDPLAVELALLNVRRNRVAKQVKVTLGSFPADGPSRIEPVE
                     LLVANLRSDTLIELLQAGLAAWVAPTGRLLLSGLLDTDLPNLRTAAEKASLQELQVRA
                     TDDWRTVLFQLA"
     gene            complement(4103..4354)
                     /gene="infA"
                     /locus_tag="VI520_01395"
     CDS             complement(4103..4354)
                     /gene="infA"
                     /locus_tag="VI520_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HLF36568.1"
                     /translation="MTKKEEKIEVDGIVVEALPATQFRVELDNGHVVLAYLSGKMRRH
                     YIRILRGDRVRVELSPYDLTRGRIAYRFKKGGKVIEETR"
     gene            complement(4357..4569)
                     /gene="rpsU"
                     /locus_tag="VI520_01400"
     CDS             complement(4357..4569)
                     /gene="rpsU"
                     /locus_tag="VI520_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLF36569.1"
                     /translation="MATVVLRPNESQDALLRRFRKKVAKSGILSTVRRKRWFVSKSEM
                     RRIERKKSIRRLKRRQRKNVSFDQGR"
BASE COUNT          900 a         1455 c         1495 g          820 t
ORIGIN      
        1 gacgaccatg ccgaaggtca ggtgatcgaa gtggtgcagc gaggttaccg catgggagat
       61 cgagtgctgc gaccggccct ggtacgggtc gccaaataag gtgaactaaa catgggacgg
      121 attattggga tcgacctggg caccaccaat tcagtcgttg cggtcatgga gggcggcgag
      181 ccgaccgtga tcacttcctc ggagggcagc cgactgatcc cctcggtggt tgcgatcaat
      241 ccgaagactg gcgagcgctt ggtcggccaa gttgcccgcc ggcaagcaat cgtcaatgcg
      301 gacaacacga tcttttcgat caagcgcttt atgggccgca agcacagcga tcctgaggtt
      361 caacgggcgc tcgatgtagt tccctataag gtgactggtg cggccaatgg agatgtccgg
      421 gttcacatgg gaggcaagga atacgcgccc cccgaggttt cggccatgat cctggccaag
      481 atcaaggccg acgcagaggc ttatatcggg gaggcggtaa cccaggcggt catcacggtc
      541 ccagcttact tcaatgactc gcaacgcaat gcaaccaagg acgcaggcaa gatcgccggg
      601 ttggaggtgc tgcggatcat caacgagccg actgcctctt ccttggccta cggcttggat
      661 cgaaagaccg acgagaccat cgcggtattc gatctgggcg ggggcacctt cgacatctcg
      721 atcctggatg tcggcgacgg cgtgttcgag gtcaagtcca ccaacggcga cacgtttctg
      781 ggcggagacg acttcgacca acgcatcatc gactgggtgg tggaggagtt tcgccgcgac
      841 cagggcatcg acatgcgcca ggaccgccag gggctccagc gactgcggga agccgccgag
      901 aaggccaaga tcgagctctc caccgtggcc cagaccgaga tcaacttgcc cttcatcacc
      961 gccgatgcat ccggtcccaa gcacctgaac ctgaccttaa cccgagcaaa gctggagcag
     1021 ctaaccgagg atctgatcga gcgctgcgtg gccccctgca acatggcact ccaagacgcc
     1081 gagctcaagg ccgcacagat tgacgaagtg attctggtgg gagggatgac ccgcatgccg
     1141 gcggtgcagg aggcggtgcg taagattttc gagaaggaac cccataaggg ggtgaacccg
     1201 gatgaggtcg tggcggtcgg ggccgccatc caggcgggcg tgctgggcgg ggaggttaag
     1261 gacatcctct tgctggatgt cactccgctt tccctttcca tcgagactct gggcggcgtg
     1321 gcgaccgccc tgatcgaacg caacaccacc atcccggctc gaaagactca ggtattttca
     1381 accgcgggcg agaatcagac cgaagttgag atccacgtcg tgcaaggcga gcgctcgatg
     1441 gcggccgaca acaagtcgct cggcaagttc cggctggacg gaatcccgac cgccccccgc
     1501 ggggtgcccc agatcgaggt caccttcgac atcgacgccg atggaatcct gaacgtcagc
     1561 gccaccgaca aggcaacctc taggactcaa cacattacga tcactgccag ctcggggttg
     1621 tccgaagtcg aagtcgagca gatgcgaacc gacgccgagc tgcacgccgg ccaggatgcc
     1681 gagcggcgag accgggccga agcccgcaac gtggccgaca acgcgctcta caacgcccgc
     1741 aaggtcctcg atcagaacga aggccacatc ccccagggag ctcgggcgga cctccaggcc
     1801 aagctgcgaa acttggaaca ggcaatggag gaggcggaca ccgagcgcct gcgaaagatg
     1861 tccaatgaat tgctcgcggc ggtccgagag gtcgccgcaa gagctcagtc cggccgcgcc
     1921 cccggaggag agaatggttc ctcctccgga gacggggttg accgcgagta tttcgagagc
     1981 taatccggcc gggcgaaggc ggatggttgc gccttcgccg gccttttaag ccagatggca
     2041 gataaacgag actactacga agccttgggg gtggggcgag atgcctcagc cgacgacttg
     2101 cggagcgcct accgccggct cgccaagcag taccacccgg atgtgaatca cgaaccgggg
     2161 gccgaagacc gcttcaagga aatcaacgag gcgtacgggg tgctctccga cgatcagcgg
     2221 cgggcggcct acgatcgatt cgggcacgcc gggctgaagg gtatgcctac cgatttcgac
     2281 tttggattca ccgacatctt cgaggagttc ttcggattcg gcatgagcgg tcggcgccgt
     2341 cgcggcgccc ctcgccgcgg cgcggatctg cgctacgacc tgcggcttga gttccaggaa
     2401 gctgtcttcg gggccgagaa ggagatcgag tttgtccgaa gggacatctg ctcgagctgc
     2461 cggggcacgg gagccgagcc aggcacgact cccgttcgct gcccgacttg cgagggttca
     2521 ggcgaggtcc gccaggtgcg gcagaccttc ctgggctcga tggtcaacgt gacgacctgc
     2581 cccaattgcg gcggccgagg cgagctggtc tcgtctccct gccgggtctg ccacagcagc
     2641 gggatcgagc aacaacgggt cgtccgccag attcccattc cggccggagt cgacgacgga
     2701 acccaggttc gggtcaccgg cgagggcgag cccggcccca acaacggtcc ccggggcgat
     2761 ctctatgtgg tggttcaggt cctgccccac ccgtactttc ggcgacgcaa ggatgagatc
     2821 ttggttgacc tctcgatcaa catcgcccaa tccgccctcg ggtcggagat cagcgtcccg
     2881 accctggacg gcgaggagaa actgcgggtc cccgccggca cccagccagg gaaggtcttc
     2941 cgactcaagg gcaagggcgt cccgcacttg agatctaacg gtcggggcga ccagttggtg
     3001 atcatctcgg tcgaggtccc gaaatcgctc tcccccgagc agcgagagct cttcgagcgg
     3061 ctggctctaa ccctcggctc cgaggtcaag cctcaggccc gcagcctgct ggatgatctc
     3121 aaagacctaa tcggcgggct ggtggattga actggttgga ggtctctctc cggctccgcg
     3181 cggaattggc ggaacccgca gcggagctgc tcgggcgtct cgccccgaac ggagtcgcca
     3241 tcgaaggatt ggacgaccag gggcaggcgg tcttggtgcg ggcctggctc ccggtcgacg
     3301 gaagtctcgc cagccgccaa gccgaactcg aagaaggcct ctggcacctt ggccagattc
     3361 agccctttcc atcgcccatc ttccgttcgg tcgacgagca ggactgggcc gagatctgga
     3421 agcgcgagta tcagccgctg ccgatcgggc gtcgcttgat ggtgctaccg agttggagca
     3481 cagccgaccc tcgggggcgc ctggtcgtgc agctggagcc ggggatggcc ttcgggaccg
     3541 gggcccatcc cacaacccgc cattgtctcg agctcatcga ggagactctg cgcccaggcg
     3601 agagagtcgt ggacctcgga tgcggctccg ggattctgtc catcgctgcc gcccggctag
     3661 gtgctcggcg ggtcttggcg cttgacaccg acccgctggc ggtggaactg gcgctgctca
     3721 acgtccgtcg caaccgggtt gccaagcagg tcaaagtgac cctgggctcg tttccggccg
     3781 acggaccgag tcggatcgag ccggtcgagc tgctggtggc caacctccgt tccgataccc
     3841 tgattgagct gctacaggcc gggctggccg cctgggtcgc acccaccggt cggttgctgc
     3901 tttccggatt gctggacacc gacctcccca atttgagaac cgcggctgag aaagcctcgt
     3961 tgcaagagct gcaggttagg gcgaccgacg attggcgaac cgtcctgttc cagctggcct
     4021 gacccggagc aggaatcact ccgcccccaa gcagcggggg cggttttgat tctcaaaccg
     4081 aggcgaaggt cagaggcgaa ccttagcgag tctcctcgat caccttgccg cccttcttga
     4141 accggtaggc gattcgaccc cgggtaagat cgtacggcga tagctcgacc cggacccggt
     4201 ccccacgcag gatgcgaatg tagtgccggc gcatctttcc agaaaggtaa gccagcacta
     4261 catgcccgtt gtcaagctcc acccggaatt gagtcgcggg caaggcctcg accacgatgc
     4321 cgtcgacctc gatcttctct tccttctttg tcacggctat cgcccttgat cgaaggatac
     4381 gttcttgcgc tggcgtcgtt tcagccgacg aatggacttc ttgcgctcga tccgccgcat
     4441 ctcactcttt gagacaaacc agcgcttccg gcgtacggtg ctgaggatgc cgctcttggc
     4501 taccttctta cgaaaccgtc tgagcagtgc atcctgggat tcgttcggcc gcaggactac
     4561 tgtggccatc tcaacctcac ctcacttccg aattagagct gttggtcaac attcgcgggc
     4621 tgcgcccact gcaggtcgag gcgctctttg gtgggctcga aggagctcac
//
LOCUS       DATDPQ010000061         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2896465, whole genome shotgun sequence.
ACCESSION   DATDPQ010000061 DATDPQ010000000
VERSION     DATDPQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896465"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VI520_01405"
     CDS             <1..717
                     /locus_tag="VI520_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLF36570.1"
                     /translation="RPELRNALNWEAMELFAKAVRESASDGQLRALVITGSDGAFSSG
                     GDLYELDQYSSRLDGARLATVMGEALRDLDGLPVPTLAAVEGLALGGGAEIALACDLR
                     VLSETASLGLMHIKLGIIPAWGGGQRLQRLVGYSLALEWLSAARVLNASEAHAHRLAN
                     RVVAAGSALEVAQSIAAKIATFDPAAVRALKRALQEGLSQPLPQALATERALFPDLWA
                     APAHLDASTRFVSRRNHQPR"
     gene            755..1555
                     /locus_tag="VI520_01410"
     CDS             755..1555
                     /locus_tag="VI520_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36571.1"
                     /translation="MFNKPRRSKLSRALEQATDPELRSLRAEVARRQERTAELELELF
                     DLRVSLAEFERDLEIRVRPLERELLDLRAKLDQLRREAARRAQWGERATQEEIPDVVE
                     QFRRAWTPRFQQDSEPAKPAPSESQQVELRSLYRELAKRFHPDLTTDPKQKPWREEMM
                     AKVNLAYEANDIAALRGLQQQVERPAQEAEVSRDQLLTDLHSEIRRLDRVVVGLQSEV
                     DRLERSEVALLKLDSVKARLNGYDLLASMARDFGTQIAQVRAELASLA"
     gene            complement(1564..>2185)
                     /gene="lexA"
                     /locus_tag="VI520_01415"
     CDS             complement(1564..>2185)
                     /gene="lexA"
                     /locus_tag="VI520_01415"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HLF36572.1"
                     /translation="LDFIRKFGLRSGYPPSIREIGEAVGISSTSVVNYNLNRLVEHGY
                     LDRDQNVSRGIRLTERLSEVASQLTEVVRIPLVGRIFASEPVPLPSTDSPMFSTDEAI
                     EITRDLLSSTEDLFALQVRGDSMIDALVNDGDIVVMQRQPEWRNGDMVAVWLKDKEET
                     TLKHIYRERGGIRLQPANPTMRPIFIEKPENLEVQGKVMLVLRQLA"
BASE COUNT          415 a          692 c          668 g          410 t
ORIGIN      
        1 cggccggagc tgcggaacgc cctaaattgg gaggcgatgg aactcttcgc caaggcggtc
       61 cgggaatctg ccagcgatgg gcaactgcga gccctggtga tcaccggatc ggacggcgcg
      121 ttcagctccg gaggcgacct ctacgagctc gatcaatatt ccagccgact agacggtgcg
      181 cggctcgcga cggtcatggg cgaagcgctg cgcgatctag atgggctgcc ggtcccgacc
      241 ctggccgctg tcgaaggcct cgcgcttggg ggcggtgccg agatcgcgct ggcctgcgat
      301 ttgcgagtcc tttctgaaac cgcctcactc ggattgatgc atatcaaact ggggatcatc
      361 cctgcctggg gaggcggtca gcgtctgcag cgcctggtcg gttactcgct ggccttggaa
      421 tggttgagcg cggcccgagt gctgaatgcc tccgaagcac acgcccaccg gctggcgaac
      481 cgggtagttg ccgctggcag cgccttggaa gtcgctcaat ccatcgccgc taagatcgcg
      541 acctttgatc cggccgcggt tcgagctctc aagcgtgcgc tacaggaggg actgtcccag
      601 ccactcccgc aggccctcgc gaccgagcgg gccctcttcc ccgacctctg ggcagccccg
      661 gcccacctgg atgcctcgac tcgattcgtc tcccgccgaa accatcaacc ccgctagcca
      721 ccgatgtcat tcgggtagaa tcagcccccg cacgatgttc aacaaacccc ggcgcagcaa
      781 gctctcgcgg gcgctcgagc aagccaccga tccggaacta agatcgctcc gagcggaggt
      841 cgctcgccgc caggagcgca ctgcggagct cgagctggaa ttgttcgatc tgcgggtgtc
      901 gctggctgag ttcgagcggg acctggaaat ccgggtacgc ccattggagc gagaactgtt
      961 ggacctgcgg gccaagctcg atcagctccg acgggaggcc gccaggagag cccagtgggg
     1021 cgagcgagca acccaggaag agattccgga tgtcgtcgag cagttccgca gggcctggac
     1081 accgcgcttt cagcaggaca gcgaaccagc caagcctgct ccctccgaat ctcagcaggt
     1141 cgaactgcgc agtctgtacc gcgagctcgc caaacgcttc catcccgatc tgactaccga
     1201 ccccaagcag aagccgtggc gcgaggagat gatggccaaa gtgaacctgg cctacgaggc
     1261 caacgacata gccgcgctgc gggggttaca gcagcaagtt gagcggccgg ctcaggaagc
     1321 tgaagtaagt cgcgatcagc tgcttaccga tctgcactcg gagatccggc gtctcgatcg
     1381 ggttgtcgtc ggactgcagt ccgaggttga tcggctggag aggtccgagg ttgcgctgtt
     1441 gaagctggac tcggtcaagg ctaggctcaa cggctacgat ctgctggctt cgatggcccg
     1501 cgactttggg acccaaatcg cacaagtccg ggccgaattg gcttccctgg cctagcgatt
     1561 tcgctacgca agctgccgca gaaccagcat cacctttccc tggacttcca ggttctctgg
     1621 tttttcgatg aagatcggcc gcatcgtcgg attggccggc tgcagccgga tcccccctcg
     1681 ttctcgatag atatgcttca aggtggtttc ttccttgtct ttgagccaca ccgccaccat
     1741 gtccccgttg cgccactccg gctggcgctg catcacgacg atatccccgt cgttgaccag
     1801 agcgtcgatc atcgaatccc ctcgcacttg caatgcaaag agatcttcgg tgctgctgag
     1861 caaatcgcgg gtaatctcga tcgcctcgtc ggtgctgaac atgggtgaat cagtacttgg
     1921 cagcggaacc ggctcgctgg caaagattcg gccgaccaat ggaatccgga cgacctccgt
     1981 cagctgtgaa gcaacttccg acaagcgctc agtgagccgg attccacggg agacgttttg
     2041 atctcgatcc aggtagccgt gctcgaccag ccggttgagg ttgtagttga cgaccgaggt
     2101 ggacgagatt cccaccgctt ccccgatctc tcggatcgaa ggcggatatc cacttcgcag
     2161 accgaacttc cgaatgaaat ccagc
//
LOCUS       DATDPQ010000062         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1069764, whole genome shotgun sequence.
ACCESSION   DATDPQ010000062 DATDPQ010000000
VERSION     DATDPQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069764"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..930)
                     /locus_tag="VI520_01420"
     CDS             complement(76..930)
                     /locus_tag="VI520_01420"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF36573.1"
                     /translation="MRTILPGGEPFFFLGNSGSGCLLLHGLTSAPQEMRGLGEYLAAR
                     GHTVLGLRLPAHATRPRAMLHTHWEDWLAAAEDGYHLLENNVRHVIVLGLSLGGAIGL
                     LLASSRPVAGVVAMSTPYEVPPQPRLRVLKALLRPLALLGRVIPYLPKPPPMDYRNHE
                     AAQNHLTYPVLPVRAITETAQLLTVMRARLAEVRVPVLLMHSIHDGGVSPANARSIYE
                     QLGSAQKQLLWLENSGHVLTVEPAHRQVYESAAGFVERWSQPARDGLGEPDEGAATRP
                     RRRASLGR"
     gene            complement(927..2036)
                     /locus_tag="VI520_01425"
     CDS             complement(927..2036)
                     /locus_tag="VI520_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HLF36574.1"
                     /translation="MDHQKRIERLSKLLRAEGLEALAVLPGPNLVYLTGLSFHLMERP
                     VVGLFSASAPPRLIVPEFEKPKAEAADVRMELFPYGEQEGASLSAFQAGLSGMPTDGR
                     AVGVEPLRARFHELGLLKSASPQTRFVSAASLLASLRMTKDSAELAAIRRAIEIAQQA
                     LDQTLPLIRLGMTEQELASELTVQLLRAGSEPEIPFSPIIATGPNSAHPHAIPGDRRL
                     AAGELLIIDWGATASGYISDLTRTFAVGEIDPKLAEVHRVVEQANAAGRQAVGAGVPC
                     GEVDRAARTVISSAGYGEFFLHRTGHGIGLEAHEPPYIRDGETTLLEPGMTFTVEPGI
                     YLAGRGGVRVEDNLVVTEQGGESLTSTPRELRVVG"
     gene            2062..2133
                     /locus_tag="VI520_01430"
     tRNA            2062..2133
                     /locus_tag="VI520_01430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2095..2097,aa:Ala,seq:cgc)
BASE COUNT          386 a          686 c          693 g          368 t
ORIGIN      
        1 tcaccggcgg ccgagggatc ttcgatatgc agttcagtca ctacgagact gtgccgtctc
       61 atgtggccga gggaatcatc gcccaagtga agcacgtcgg cgaggacgcg tagccgctcc
      121 ctcatccggc tcaccgagtc catcgcgggc cggctgactc cacctttcaa cgaatccggc
      181 ggcggattca tagacctgcc gatgggcagg ttccaccgtc agcacatgtc cgctgttttc
      241 cagccataag agctgcttct gggcagatcc caattgttcg tagatcgacc gggcgttggc
      301 tggagacact ccaccgtcgt gaatcgaatg catcagcagc acgggcactc gaacctcggc
      361 caaccgcgct cgcatcacgg tcagcaattg ggcggtctcg gtgatggcgc ggaccggcag
      421 caccgggtag gtgagatggt tctgagcggc ttcatgattt cggtaatcca tcggaggcgg
      481 cttgggcagg tatggaatca ctcgaccgag cagcgcgagc ggccggagca gggccttcag
      541 aacacgcagc cgaggctggg gtggtacttc gtagggtgtc gacatggcaa ccacacccgc
      601 gaccggccgg ctggacgcca ggagcaagcc gatcgcgcca ccgagcgaga ggcctagcac
      661 gatgacgtgg cgcacattgt tctcgagcaa atggtagccg tcctcagccg ccgccagcca
      721 atcctcccag tgggtgtgga gcatagctcg aggccgggtg gcatgggccg gcaggcgcag
      781 cccgagaact gtgtgaccgc gcgccgcgag gtactcaccc aagccgcgca tctcctgagg
      841 agcgctggtc agcccatgca gcagcaggca tccactgccg gaattgccga ggaagaaaaa
      901 cggttccccg ccggggagaa tggtcctcac ccgacgactc gcaactcccg cggagtgctg
      961 gtcaaacttt cgccgccctg ctcggtgacg acgaggttat cctccacccg gactcccccg
     1021 cggccggcca ggtagatccc cggttcaacc gtaaaagtca tccccggctc gagcaacgtg
     1081 gtttcgccgt cacggatgta tgggggctcg tgcgcctcga gtccgatccc gtgcccggtc
     1141 ctatggagga agaattcccc gtatccggcc gacgaaatca cggtccgggc cgcccggtcg
     1201 acctcgccgc agggcacgcc cgcaccgacg gcctgccggc ccgccgcgtt ggcttgctcg
     1261 acgactcgat gcacctcagc cagtttcggg tcgatctccc cgaccgcaaa ggtccgggtg
     1321 agatccgaga tgtagcctga agcggttgcc ccccagtcga tgatcaggag ctccccggct
     1381 gccagccgcc ggtctcctgg aatcgcgtgc gggtgggccg agttgggccc ggtggcgatg
     1441 atcgggctga aggggatttc cggctcggaa cccgcccgca acagctgcac ggtcagctcg
     1501 gaggccagtt cctgttcggt catcccgagc cggatcagcg ggagggtctg gtccagggct
     1561 tgctgggcaa tctcaatcgc tcggcggatt gccgccaatt cggccgaatc ctttgtcatt
     1621 ctaaggcttg ccagcaggct ggctgccgaa acgaaccggg tctggggcga ggcagacttg
     1681 agcagtccaa gctcatggaa ccgggcccgc agcggctcga ctcccaccgc ccggccgtcg
     1741 gtcggcatcc cgctcaaccc ggcctggaag gcagagaggc ttgccccttc ctgctcgccg
     1801 taggggaaga gctccatgcg cacgtccgca gcctcggcct ttggcttttc gaattcagga
     1861 acaatgagac ggggcggtgc gctggccgag aacaagccaa ccaccgggcg ctccatcaaa
     1921 tggaaggata gcccggtgag atagaccagg tttggcccgg ggagcacggc cagtgcttcg
     1981 agcccctcgg ctcgcaagag cttggatagc cgctcgatcc gtttctggtg gtccataggg
     2041 gatttgcggt attataagcg ggggcctgta gcgcagttgg gagcgcgcat caatcgcact
     2101 gatgaggtcg cgggttcgaa tcccgccagg tcc
//
LOCUS       DATDPQ010000063         6161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1850096, whole genome shotgun sequence.
ACCESSION   DATDPQ010000063 DATDPQ010000000
VERSION     DATDPQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850096"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..289
                     /locus_tag="VI520_01435"
     CDS             74..289
                     /locus_tag="VI520_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36575.1"
                     /translation="MRTRRIVLWVISLGFGVVGTIGTVLVFGSDFNRFWTNFLFKGDL
                     PLLLLVGFGSLAFIWLDLILQTQYLKR"
     gene            360..1544
                     /locus_tag="VI520_01440"
     CDS             360..1544
                     /locus_tag="VI520_01440"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HLF36576.1"
                     /translation="MTDSRLAGFYNLDLAQRQALLSASTGLDLSPIFGSGGLDPARAD
                     AMIENAVGVFGLPLGIAANFVVNGKDVLVPMAIEEASVVAGASHIAKLVRESGGFRAE
                     SDPSHMIGQIQLMDLDDPVGAESALMAAETEILAAADAANPRLLELGGGARSIEVRRI
                     EQSSFAPMLVVHLIYDTVDSMGANSVNTVLETIAPLIEAISGGRAHLRILSNLADRRL
                     ARASCSIQPQTLSFEQYDGEQVRDRILEAYAFAAADPYRAATHNKGIMNGIDAVVIAT
                     GNDWRAVEAGAHAFAARSGRYRPLSRWSTDDRGQLVGELELPMAIGTVGGATRSHPVA
                     QVCLALMQVQTARELAGIVAAVGLAQNLAALRALATEGIQRGHMGLHARQLTRVEGTR
                     HE"
     gene            1555..2601
                     /locus_tag="VI520_01445"
     CDS             1555..2601
                     /locus_tag="VI520_01445"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HLF36577.1"
                     /translation="MKPYRPVGFTGYGAYIPQFRLPASEVARIWTGGQGGLPVEEKAV
                     AGLDEDVITMSIEAARNALARSAISPSAIRAVWVGSESHPYAVKPTSTVVAEAIGATP
                     HTQAADWEFACKAGTEAIQAAIGFVGSGMADYALAIGMDTAQGRPGDALEYTAGAGGA
                     AFLLGPAETSLCTIEGSYSYATDTPDFWRRANETYPEHGERFTGEPAYFKHITTAAES
                     LMQSTGTTASDFRYVVFHQPNTKFPLRVGKELGFREDQIETGLLVPQIGNTYAGSAMV
                     GLTAILDVAEAGDRILMVSYGSGAGSDAFSLQATGLLSARRSAAPDTMSYIRRRTEID
                     YATYARFRGKLVTK"
     gene            2601..3779
                     /locus_tag="VI520_01450"
     CDS             2601..3779
                     /locus_tag="VI520_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HLF36578.1"
                     /translation="MDGHSQVLVAGIGMTPVGEHWDVSLRNLALDAISAAMQDAGGLR
                     PEALYVANMLAPALSGQSHLGALLADFAGLRGIEATTVEAAGASGGVAARQAYLAVAS
                     GAVEVALVLGVEKVTDKDSGQVEAALATASDADYEAIQGVTVGAQAALLMRRYLYEHH
                     APQDALAGFSLVAHQNAVANPNAMFRKAISLEQYSKAPMVSDPLNTYDAAPMADGAAA
                     LLLARAETVQNPEAHPAVEIAGSAVATAAMSVHDLLDPLGLPAAARSVQSALLRAAIQ
                     TEQVNLFELHDRFSIFAALSLEAAGFAERGGGWLLAKQAEIGLNGRIPICTYGGSKAR
                     GEPGAATGVYQLAEAVSQLEGRAGKNQVQGASVALVQCLGGAGATAATHVLRRTSNGI
                     "
     gene            3816..4226
                     /locus_tag="VI520_01455"
     CDS             3816..4226
                     /locus_tag="VI520_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HLF36579.1"
                     /translation="METSRHWRLRQQRYALVGEECPNCELKIFPPRDICPDCGHEART
                     LFQFSGRGEVYSHTTVYQAPAGHTEAAPYPLAIVRLEEGPLVTAQLTDLAHQPVEIGM
                     PVEMVTRKLKSDGERGMLVYGYKFRPLLPRGEPS"
     gene            4399..5001
                     /locus_tag="VI520_01460"
     CDS             4399..5001
                     /locus_tag="VI520_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLF36580.1"
                     /translation="MTVTRFLVASALLALVAACTRSASTPPPGEEVVPSQDSQQATME
                     AVRSALLTQTAEAGGPVEQATPVPSLAPTGTAVSDQVTATEGAEEFVEYTVKAGDWLF
                     QIAESFGVDPQAIVDLNGLASESQVQAGLVLKIPPSSGVPATPISGTSVAAGQIHVVK
                     LGEWIWQIARIYGVDPQAIIDANNLANPGLIFPGMELKIP"
     gene            5071..5613
                     /locus_tag="VI520_01465"
     CDS             5071..5613
                     /locus_tag="VI520_01465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLF36581.1"
                     /translation="MGTRVTASETLYRGKVFDLRKDQIVFEDGRESSVELVVVHPGAV
                     ALIPVDELEQIWFVRQYRHASGGELLELPAGTLEPGEPPEQCARRECQEEIGMFPGKL
                     EHLGGFFLAPGYSTEYLEVYLASELRPGPRPPDADEQIQVEKLSLGQVAELIRKGELR
                     DAKSLASITLAGQRLGLPSR"
     gene            5694..6032
                     /gene="rplS"
                     /locus_tag="VI520_01470"
     CDS             5694..6032
                     /gene="rplS"
                     /locus_tag="VI520_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLF36582.1"
                     /translation="MTDLVQAPANPNIPQLRPGDSVSVHVRVREGDSERVQEFRGTVI
                     RLRKGGNNANFTVRRIASHGIGVERTFLLRSPRIEKVEVLRQAHVRRAQLYYLRGRKG
                     KRARLAAKRS"
BASE COUNT         1091 a         1936 c         2005 g         1129 t
ORIGIN      
        1 ggaagtacct gggcgacttg accgctctgc ctggccaatt tagaattccg gctgtcccgg
       61 ggctgaaagg gctatgagaa cccgtcgcat cgttctgtgg gtgatttcgc tcggctttgg
      121 cgtggtgggc acgatcggca cggtccttgt tttcggctct gacttcaatc gcttttggac
      181 caacttcctg ttcaagggag acttgccgct gctgctgctg gtggggtttg gcagcctggc
      241 gttcatctgg ctcgacctga tcttgcaaac ccagtacctc aagcgctaga ccgtctcccg
      301 gtttcctgat agtttttgcc agctatccgc cgatataagc agtcggtgcg ctgccaccga
      361 tgactgactc gcgtctggcg gggttctaca acttggacct ggcccagcgc caggcgctgc
      421 tctccgcgtc caccgggctc gacctctcac cgatcttcgg atccggcggc ctcgaccctg
      481 cccgggcgga tgccatgatc gagaacgccg tgggggtgtt cgggctgccg ctcggaatcg
      541 cagccaactt cgtcgtcaac gggaaggacg tcttggttcc catggccatc gaagaggctt
      601 cggtcgtggc tggggcttcc catattgcca agctggtccg tgagtcgggc ggatttcggg
      661 cggaatccga cccctcccac atgatcgggc agatccagct gatggatctc gacgatccgg
      721 tcggcgccga gagtgcgctg atggccgcgg agaccgagat cctggccgcc gctgacgcgg
      781 ccaacccgcg gctgctcgaa ctgggaggcg gggcgcgcag catagaggtt cgccggatcg
      841 agcagtcctc attcgctccg atgctggtgg tgcacttgat ctatgacacg gtcgattcga
      901 tgggtgccaa ttcggtcaac acggtgctgg agaccatcgc cccgctgatc gaggcgatca
      961 gcggcggccg ggcccacctt cggatcctct ccaacctcgc cgaccggagg ctggccagag
     1021 cgagctgcag cattcagccg caaaccctga gcttcgagca atacgatggc gagcaggtgc
     1081 gcgaccggat cctcgaggca tatgcctttg ccgccgcgga tccgtatcgg gccgccaccc
     1141 acaacaaagg aattatgaac gggatcgacg cggtggtgat tgccaccgga aacgattggc
     1201 gggcggtgga agccggggcg cacgccttcg ccgcccgcag tggccgctac cgaccgttga
     1261 gccgctggtc gactgacgat cggggacagt tggtgggcga gctcgagctt ccaatggcga
     1321 tcgggaccgt ggggggcgcc acccgatctc acccggtcgc gcaggtctgt ctggcgctca
     1381 tgcaggtcca gaccgcccgg gagctggccg gaatcgtggc cgctgtgggg ttggctcaaa
     1441 acctggcggc cttgcgtgcg ctggctacgg aagggattca gcgcggtcat atggggctgc
     1501 acgcccggca actgacccga gtcgagggta cccgtcatga gtgagaatca tctaatgaaa
     1561 ccctaccggc cggttggatt caccggctac ggggcctaca tcccgcagtt ccggcttccg
     1621 gccagcgagg tggctcggat ttggaccggc ggccaaggcg gactcccggt cgaggagaaa
     1681 gcggtcgccg gtttggatga ggacgtgatc acaatgtcga tcgaggccgc ccgcaatgcg
     1741 ctggcccgat ccgctatcag cccttccgct atccgggcgg tctgggtcgg ttcagagtcg
     1801 catccgtacg cggtcaaacc gacctcgacc gtcgtggcgg aggcgatcgg cgctaccccg
     1861 cacactcagg ctgccgattg ggagttcgcc tgtaaggccg gaaccgaagc cattcaggct
     1921 gccattggtt tcgtgggctc cgggatggcc gactacgcgc tggcaattgg tatggacacc
     1981 gcccaaggtc ggccggggga tgccttggaa tacaccgccg gcgccggcgg cgcggccttt
     2041 ctgcttgggc cggcagagac ctccctctgc acgatcgaag gttcctactc ttacgccacc
     2101 gacaccccgg acttttggcg ccgggcgaac gagacctacc cggagcacgg ggagcgcttc
     2161 accggcgagc cggcctattt caaacacatc acgaccgcgg ccgagtcgct gatgcagtcg
     2221 accggaacga ccgccagcga tttccgctat gtcgtcttcc accagcccaa tacgaaattc
     2281 ccgctccggg tgggcaagga gctgggtttt cgggaagacc agatcgagac cggcctgctg
     2341 gtaccgcaga tcggaaacac ctatgccggc tcggcgatgg tgggactgac cgccatcctc
     2401 gacgtcgccg aagcagggga tcggattctg atggtgtctt atggatcggg agccggttcg
     2461 gatgccttca gcctacaggc caccgggctg ctctccgctc gccgctccgc cgctccggat
     2521 acgatgagtt atattcgccg gcgaaccgaa atcgattacg ccacctacgc ccgcttccgg
     2581 gggaagttgg tcacgaaatg atggatggac actcgcaggt tctggtcgct gggatcggga
     2641 tgaccccggt gggcgagcac tgggatgtgt cgctgcgcaa cctggcgctg gacgcgataa
     2701 gtgccgccat gcaagatgcc ggcggtctgc gccccgaagc gctgtacgtg gccaacatgc
     2761 tggcgccggc gctctccggg cagtcacacc tgggggcctt gctggccgac ttcgctggtc
     2821 tgcggggtat cgaggctacg acggtcgagg ctgccggagc atcgggcggg gtggccgccc
     2881 gtcaggccta cttggccgtt gcttcaggcg cggtggaggt agccctggtc ctaggcgtgg
     2941 agaaagtcac cgacaaggac agtggacagg tcgaggcggc gctagccacg gccagcgacg
     3001 cagactatga ggccatccag ggagtgaccg tcggtgccca ggccgccctg ctgatgcggc
     3061 gctacctcta cgagcaccac gccccgcagg acgccctggc gggattcagc ctggtggctc
     3121 accagaatgc cgtcgccaat ccaaacgcga tgtttcgcaa ggcgatcagt ctggaacagt
     3181 actccaaggc acccatggtc agcgatccgc tcaatactta cgatgccgcg cccatggccg
     3241 atggcgccgc ggcattgctc ctggcccgag ctgagaccgt ccagaacccc gaagcgcacc
     3301 cggcagtcga gatcgccggc tcggcggttg ccaccgccgc catgtcggtc catgacctgc
     3361 tcgatccact gggcctgccg gccgcggcgc gcagcgtgca atccgccctc ctgcgagcgg
     3421 caatccaaac tgagcaagtc aatttgttcg agctgcacga tcggttctcg atcttcgccg
     3481 ccctttcgct ggaggccgcc gggttcgccg aacgcggggg cggttggctg ctggccaagc
     3541 aggcggagat cggcttgaac ggccgcatcc caatctgcac ctacgggggc tccaaggccc
     3601 gcggggaacc cggcgcggcg accggcgtct accagttggc cgaggccgtc tcgcaattgg
     3661 aggggcgcgc cggtaaaaac caggtccaag gggcctcggt cgcgctggtc cagtgcctgg
     3721 gcggcgcggg cgcgaccgcc gcaacccacg tcttgcggcg cacttcaaac gggatctgat
     3781 agtttctgag aggcggggca gaatagatta gcagcatgga gacctcacgc cattggaggc
     3841 ttagacaaca gcgttatgca ctggtcgggg aagagtgccc caactgtgaa ttgaagattt
     3901 ttccgcctcg ggacatctgc ccagactgtg ggcatgaggc tcggaccctt tttcagttca
     3961 gcggacgggg agaggtctat tcccacacca ccgtgtatca agctcccgcc ggccacactg
     4021 aggccgctcc ttacccgctg gccatcgtcc gcttggaaga gggaccgctc gttaccgccc
     4081 agctgaccga tctggcccat caaccggttg agatcgggat gccggtcgag atggttaccc
     4141 gcaaactcaa gtcggatggc gaacggggca tgctggtcta cggatacaag tttcgcccac
     4201 tgcttccacg gggcgaacct agctaaacga cccgctattt accttgactg cggccgcctc
     4261 aaaggcggcc gattggtttt cccctcccgt cgggcacaac tcttgcagct ttacctcgct
     4321 tgcacagcca attcgcttgt gctaggatgc ccggccgttc cgaggagacc gggggcggaa
     4381 ccctcccagg aggagcaaat gactgtcact cgattcttgg tcgccagcgc tctactcgcg
     4441 ttggtcgccg cttgtacccg ttcagcttcg accccacccc caggtgagga ggtggtcccg
     4501 tcgcaggata gccagcaggc gacaatggaa gcggtgcgct ccgccctgct cacacagacg
     4561 gccgaggcag gtggacctgt cgagcaggct accccggtcc cgagccttgc tccgactgga
     4621 accgccgtgt ccgatcaagt gaccgcgacg gagggggcgg aggagtttgt ggaatacacc
     4681 gtcaaagccg gcgattggct cttccaaatc gctgagagct ttggggttga cccgcaggcc
     4741 atcgtggacc tgaacggatt ggcctccgaa agccaggtgc aggccggcct ggtgctgaag
     4801 atccctccca gctcgggagt cccggccacg ccgatctccg gcaccagcgt ggcggccggc
     4861 caaatccatg tggtcaagct aggagagtgg atttggcaaa tcgcccggat ctacggagtg
     4921 gatccacaag cgatcatcga cgccaacaac ctggcgaacc cgggcctgat cttcccgggc
     4981 atggagctta agatcccgta agcgagtagt gatgctgagc aaccgacggg gcggccgaat
     5041 tggccgcccc gatttcattg gaagggaaat atgggaactc gtgtgacggc ctcggagact
     5101 ctctaccggg ggaaagtttt cgaccttcgg aaggatcaga ttgtttttga ggacggacgt
     5161 gaatcctcgg tggagttggt ggtcgtccat ccgggggcgg tggccctgat cccggttgac
     5221 gagctagagc agatctggtt cgtgcgccaa taccgacatg cctccggggg tgagctctta
     5281 gagctgccgg caggaacttt ggaacccggg gaaccgcctg aacagtgcgc ccggcgggaa
     5341 tgtcaggaag agatcggaat gtttcccgga aaactggagc acctgggagg gttcttcctg
     5401 gctcccggat actcgactga atacttggag gtgtacctgg ccagcgagct tcgcccgggt
     5461 ccgcggccgc cggatgccga tgagcaaatc caggtggaga aactgagcct ggggcaggtc
     5521 gccgaactga tccgtaaagg cgagctgcga gatgccaaga gtctggcgtc catcaccctg
     5581 gcgggccagc gcctgggcct cccgtcgcgc tgattcggcc tccagatgtg catggtatac
     5641 ttccgggccg cgccgcccgc atccaagggg cggcgggcat tctgtaggcc aaaatgaccg
     5701 atttagtgca ggccccggcc aatccgaata tccctcaact gcggcccggt gactcggtga
     5761 gtgttcatgt ccgagtccgg gagggcgaca gcgagcgggt acaggaattc cgcggcacag
     5821 tgatccgcct gcgcaagggc ggaaacaatg ccaacttcac cgtccgccgg atcgcttccc
     5881 atgggatcgg cgtcgaacgc accttcctgc tgcgctcccc ccggatcgaa aaggtcgagg
     5941 ttctccgtca ggctcacgtt cgccgggctc agctttacta tctgcgcggc cgcaaaggca
     6001 agcgcgcccg gctggctgcg aagcgcagct gatcccgcct gatgagcggg gcagccgtcg
     6061 gcgtttttga ctccggcgtc ggcgggctct caatcctccg cgagatccac aaccagctcc
     6121 cgaacgagtc gctgctctac ttcgccgatc aggcaaacgt t
//
LOCUS       DATDPQ010000064         4691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_543141, whole genome shotgun sequence.
ACCESSION   DATDPQ010000064 DATDPQ010000000
VERSION     DATDPQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543141"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VI520_01475"
     CDS             complement(<1..393)
                     /locus_tag="VI520_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HLF36583.1"
                     /translation="MSAVAPPTERKKVTTKAFRLKKSKGEIITMLTAYDYATAQAVDQ
                     AGIDAILVGDSLGMVVLGYENTLPVTMDDMLHHCKAVARGAKTALLIGDMPFMSYQVS
                     VEEAVRNAGRFLQEGGMDAVKLEGGRDRA"
     gene            complement(553..1209)
                     /locus_tag="VI520_01480"
     CDS             complement(553..1209)
                     /locus_tag="VI520_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HLF36584.1"
                     /translation="MAELVSLPLSTADGRPLVHKFYRHPEGAAGLLVTFPGNHYGVDG
                     PLLYYPAKLLGAEGWDTLAVSYGFQTAMREPFADGLENLVTECVAAIAAAIESRGYSR
                     IGLIGKSLGAGVVAYLCAHRSELTTARAAYLTPALGTSFFDPFFVKTSQPAYLAVGTH
                     DRYYSAEVLERLQAQRPFELALIEGADHSLDVPGDLSASIEAAGRAVAGLLAFFRSSH
                     "
     gene            complement(1252..2625)
                     /locus_tag="VI520_01485"
     CDS             complement(1252..2625)
                     /locus_tag="VI520_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36585.1"
                     /translation="MRSRRGAFLGGLGLFGGLLLVATLAAPRVTGFSPNPDSPHVASL
                     APIRLQFNRLMDRVSVETRFEISPELPGRTSWEGNTMRFEPLEPWPAGQEIIVRLAAG
                     SRSIRFLPMLQTFQGSFKTGEARLAFLWPTDGTADLYLLTLNSGEWARLTQSEAGVLE
                     YTLARDGGSIVYAALRTDGSSELRRLDLITLDDRLVLECSAASRCQAPALSPAGDLLA
                     YERFEWQTSDSGRRIPGPRQVWLLSLEQGAEPVQVPPPGQVTSSPIWSPNGLLSFYNG
                     SLQAVGLIRPESLVPINMIPNGLGLLGAWSPNGEYLLLPEIVFADEPSTDLGTDFSSH
                     LFRVEPESAIRQDFSLGSVEDASPAYSPDGEWIAFGRKYLDERWTPGRQLWLMRSDGT
                     EPRPLSDEPDFNHSSITWHPDSASLAYMRASSVDPNLPSEIWVTDLAGSASLLLVEGG
                     YLPKWLP"
     gene            complement(2650..3705)
                     /locus_tag="VI520_01490"
     CDS             complement(2650..3705)
                     /locus_tag="VI520_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HLF36586.1"
                     /translation="MILDQPERFTELDPQDMLGEIDGLPAQLQSAWELSHVLPLPSLE
                     PLRQVVIAGMGGSAIGADLLAAYAAPYARVPILTWRNYDLPRFVGPDTLLIASSHSGN
                     TEETLSAFERALQAGVKALAVTTGGALAERAEAASVPLWRFEHAGQPRAAVGFSFGLL
                     LGALVRLGVVEFDPDELDSTLQAMRDQAVSIRAAVPVAKNLAKRMAGQLMDRWPTILG
                     ADLLAPVARRWRTQISEIAKAMAQFEELPEADHNMVAGVEHPKAMIGPTMVVFLRSSF
                     NHPRNLKRLEATRHVLMLEGFNTDIIECPGPSRLAQQWTGLHLGDYLAYYLAMAYGVD
                     PTPVFAIEDLKVQLRPS"
     gene            complement(3702..4523)
                     /gene="rsmI"
                     /locus_tag="VI520_01495"
     CDS             complement(3702..4523)
                     /gene="rsmI"
                     /locus_tag="VI520_01495"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLF36587.1"
                     /translation="MGILYLVATPIGNLEDITLRALRVLKEVQLIAAEDTRQTRKLLT
                     RYQIKTPLISYHEHNKQARLPELLQRLEGGSVALVSDAGTPALSDPGYELVKAAVGDG
                     HRVAPIPGPAAPIAALVASGLPTDRFVFLGYLPRKTGELNRLLKELLGEPRTILAFDV
                     PHRLRSTLTAMERIFGSQRPIAVCRELTKLHEEIVRGTLEQVRLQFESSEPRGEITLV
                     IGGAGSVVEWDEQAVRDAVAQRIAQGLRRSEAAREVAAEAGWPRSTVYRLAGEQK"
BASE COUNT          873 a         1527 c         1479 g          812 t
ORIGIN      
        1 cgcccgatcc cggccgccct ccagcttgac cgcgtccatt ccaccttcct gcagaaaacg
       61 gccggcattc cggactgcct cttctaccga gacctggtac gacatgaagg gcatatcccc
      121 gatcagcagt gcggttttcg ccccccgggc taccgccttg cagtgatgga gcatgtcgtc
      181 catcgtgact ggcagggtgt tctcgtatcc gagcaccacc atgcccaggg aatcgcctac
      241 cagaatcgcg tcgattccgg cctgatcgac cgcctgggcg gtggcgtagt catacgcggt
      301 gagcatggtg ataatttcgc ctttggactt cttgaggcga aaggccttgg tggtgacttt
      361 tttccgttcg gtggggggtg cgactgcaga catagctatc tcctcctacg agtgagacgc
      421 gtgggcccag ggagaaaaaa agccccgccc gggccatggg cgggacagca gaaaagcttt
      481 gcttatgctg ccgtctcggt cggggcccga tccaagcggc aggggggatt atatcgtaga
      541 acttaggggg gactaatggg acgaacggaa gaaggccagt aggccggcca ccgcccgtcc
      601 ggccgcctct atggaggcgg acaggtcgcc agggacatcc aggctgtgat cagcgccttc
      661 gatcagggcc agctcaaaag gtctttgagc ctgcagtcgc tctagtacct cggcagagta
      721 gtagcggtcg tgggttccca cagcgaggta ggccggctgg ctggtcttca caaagaaggg
      781 atcgaagaac gaggtcccca gcgccggggt caggtaggcg gccctggcgg tcgtcaactc
      841 tgaccgatgg gcgcacagat aggccacgac cccggcgccc aacgacttgc caatcaaccc
      901 gattcgggag tacccgcggc tttcgatggc ggccgcgatc gccgcaacac actcagtgac
      961 caaattctcc aacccatccg caaatggttc ccgcatcgcc gtttggaatc cgtagctgac
     1021 ggccaacgtg tcccagcctt ctgctccaag cagcttggcc ggatagtaga gcaggggacc
     1081 gtcaaccccg tagtgattcc cggggaaggt gaccaacagc ccggccgccc cctctggatg
     1141 ccggtagaac ttgtggacca gggggcgccc atcggcggtc gatagcggca gcgataccag
     1201 ctcagccatc aggccggccc agcctcggcg ggttgttggc gggcacccgc gctacgggag
     1261 ccacttcggt aagtacccac cttccaccag aagaagcgag gccgaccccg ccaggtcagt
     1321 aacccagatc tccgacggca ggttgggatc gactgagctc gcccgcatgt aggcgaggga
     1381 ggccgaatcc gggtgccagg tgatcgagga atggttgaag tccggctcgt cactcagtgg
     1441 gcggggctcg gttccatccg atcgcatcag ccagagctgg cgtccgggcg tccagcgttc
     1501 gtcaagatat ttgcgcccga acgcgatcca ctccccgtcc ggtgagtagg cgggcgaagc
     1561 gtcctctacc gagccaaggc taaagtcctg ccggatcgcc gattctggct cgacccgaaa
     1621 caggtgactg gagaaatcag ttcccaaatc cgtcgatggt tcatcggcga agacgatttc
     1681 ggggagcagc agatactcgc cgttcggcga ccaggcgccc aacaagccca gtccgttggg
     1741 aatcatgttg atcggaacca gactctccgg gcgaatgagg ccgacagcct gcagcgagcc
     1801 gttgtaaaac gacagcaggc cgttgggtga ccaaattggg gagctcgtga cctgacccgg
     1861 cggtgggacc tgcaccggtt cggcgccctg ctccaggctc agcagccaga cctgacgcgg
     1921 gcccggaatt cgccgccccg aatcgctcgt ctgccactcg aagcgctcgt aggccaacag
     1981 gtcgcccgcc ggcgaaaggg cgggggcctg acaccggcta gcggccgagc actccaatac
     2041 caagcggtca tctagagtga tcagatccag gcggcgcaac tcggaggacc cgtccgtgcg
     2101 caacgcagca tagacaatcg aacctccatc ccgcgccagt gtgtactcaa gcacgcctgc
     2161 ctcgctctga gtcaggcgcg cccactcccc actattcaga gtcaacaggt acaggtcggc
     2221 cgttccatcc gtgggccaca agaacgccag ccgcgcctcg ccggtcttga atgacccctg
     2281 aaaagtttgg agcatgggca ggaatcggat cgagcgtgat ccggccgcca gccgcacgat
     2341 gatttcctga ccggccggcc acggctcgag cggctcgaac cgcatggtat tcccttccca
     2401 gctggtccgg cccggaagct ccgggctgat ctcaaagcga gtctcgaccg acacccggtc
     2461 catgagccgg ttgaactgca gtcgtatcgg agcgaggctc gcgacgtgtg gactgtccgg
     2521 attcgggctg aagcccgtca cccggggtgc agcgagagtt gccacgagca ggagtccccc
     2581 gaagagaccc aacccgccaa gaaaagcccc gcgtctgctc cgcatcgtct ccctgagatg
     2641 tcggccgggc tagctcggcc tgagctgcac tttgaggtct tcgatggcga acacgggagt
     2701 cgggtccacg ccataggcca tcgccaggta gtaggcgagg tagtctccca agtgtaggcc
     2761 ggtccattgc tgggccagcc gggacggccc ggggcactcg atgatgtcag tgttgaatcc
     2821 ttcgagcatc aagacgtggc gggtggcttc cagccgtttc agattgcgag gatggttaaa
     2881 ggaagaccgc aggaagacca ccatcgtcgg gccgatcatc gccttggggt gttccacccc
     2941 ggccaccatg ttgtgatcgg cctccggcaa ttcttcgaac tgtgccatgg ccttggcaat
     3001 ctcggagatc tgggtccgcc agcgacgggc caccggcgcc agcaagtcgg cccccaggat
     3061 tgtcggccag cggtccatga gttggccggc catccgcttg gccagattct tggctaccgg
     3121 gacggcggcc cggatcgaga ccgcttgatc tcgcatggcc tgaagggtcg agtcgagttc
     3181 atccggatcg aattccacaa cccccagccg gaccagcgct ccaagcagca gcccaaacga
     3241 gaatccaacc gccgcccgcg gctgcccggc atgttcaaag cgccagaggg gcacagaggc
     3301 tgcttcggcc cgctcggcca gcgcgccacc ggtcgtcacg gccaacgcct tgactccggc
     3361 ctggagggct ctttcgaagg cggacagcgt ctcttctgtg ttgccggagt gggaggaggc
     3421 aatcagcaaa gtgtccggcc cgacgaaccg aggcaggtca tagttgcgcc aggtcaggat
     3481 cgggaccctg gcgtacggcg ccgcgtaggc tgccagcagg tcggccccga tggccgagcc
     3541 gcccatccct gcgatcacga cctgccgaag tggttcgagg ctggggagtg ggagcacatg
     3601 gctgagctcc caggcagatt ggagctgcgc cggcaggccg tcgatctcac ccaacatgtc
     3661 ctggggatca agctcggtga accgctccgg ctggtccagg atcacttctg ttcaccagcc
     3721 agccgataga cagtgctccg cggccagccg gcttccgccg ccacttcccg agcggcctcg
     3781 gatcgccgca gcccttgcgc aatccgctgc gcgacggcgt cccggacggc ctgctcatcc
     3841 cattcgacga ccgaaccggc gcccccgatc accagcgtga tctcaccccg cggctcgctg
     3901 gattcgaatt ggaggcgaac ctgctccagc gttccgcgca cgatctcttc gtgcagcttg
     3961 gtcagttcgc ggcagaccgc gatgggtcgt tgcgagccga agatgcgctc catggcggtc
     4021 agggtcgaac gcaatcgatg cggcacgtcg aacgccaaga tcgtccgcgg ctcgccgagc
     4081 aactccttca gcagccgatt cagctcgccc gtcttgcggg gcaggtatcc cagaaagaca
     4141 aagcggtcgg tcggcagacc ggaggccacc agcgcagcga ttggcgccgc cgggcccggg
     4201 atcggggcga cccggtggcc gtcgcctacc gcggccttca ccaattcgta tccgggatcc
     4261 gagagcgccg gcgtcccggc atccgagacc agcgccacgc tgccaccctc gagtcgctgt
     4321 aatagctcag gcagccgggc ctgcttgtta tgttcgtggt agctgatcaa cggggttttg
     4381 atctggtagc gagtgagcag cttgcgggtt tgtcgggtgt cttccgctgc gatcagctgc
     4441 acctccttta agactcgcag cgcccgcagg gtaatgtcct ccaggttgcc aatcggagtt
     4501 gcgaccagat ataaaatgcc cacccgcgta ttgtagcaga gcgacttctt caccctctgc
     4561 gccaaagcaa agggcccgtg gcgggttgct gaccacgggc cctcagtttg cgtttgagcg
     4621 gttcaggcta cgaggtccgg ggcctcaccc tcggctccgg cctcggagcc atcctcggat
     4681 ccatcctcca t
//
LOCUS       DATDPQ010000065         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2636300, whole genome shotgun sequence.
ACCESSION   DATDPQ010000065 DATDPQ010000000
VERSION     DATDPQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2636300"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1780)
                     /locus_tag="VI520_01500"
     CDS             complement(89..1780)
                     /locus_tag="VI520_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36588.1"
                     /translation="MRLQELAAVPELAATAPARPNLISHAGAFVVSASVAGRSQILAF
                     LPGDPQVRQLTEGDWDNRDPAVSPNLDQLAFSSRRDGNWELYLLDLLTGELRRLTGTP
                     GFEGNPTWSPDGLWLAYEAHYADDFNIWILPIDGSQSPVQLTDHPAADVAPSWDPGGR
                     RIAFISDREGSPDLYLADLDKPDDRFTNLTHTVELAEADPAFSPDGSQIAYSIQLDGV
                     YGIRQLQLGQPAASAVWVGQGQDPAWVADGQALSAILRTPFTSHIVTYPIEGALGELG
                     GSLEGRISQLHWSPIPLAGLGVPAQAATESPFEVVWDQPRPEGERLAIVPLDGVRPSG
                     MSLSDAADEAFGELRARAAQAAGWDFLSQLEHAFVGINDPLPPGYAYNDWLYTGRAFA
                     ISRAAFQAGWVAVVREDFGLQTYWRIFVRTAAQDGSQGEPLRWRPWDFDLRYAGNPAA
                     YDRGGAEQENPPAGYYLDFTALAADYGFDRLPALSNWRSFYPGSRFDEFALRENMDWV
                     TAMRQLYPDSAIATPTPYQTPTPTPTNTPRPTPTPWWWRWRTPTPTPTATSTVQP"
     gene            complement(2116..>2547)
                     /locus_tag="VI520_01505"
     CDS             complement(2116..>2547)
                     /locus_tag="VI520_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36589.1"
                     /translation="RALVEYSGAAEPPVPIEAILDHPPEVFHEDFGVVDMYSNLWDAT
                     FARPPGQRGSIFVRVDLGETARRHALARETLSALITSKHGRDIGLPDLLMSELKESAS
                     YFASILLAPNAFVDAYRAQGGSEELFAESFLLPVEVAESRW"
BASE COUNT          510 a          783 c          819 g          435 t
ORIGIN      
        1 aagcaggctg ccgatcatgg gcgcaggcag aatccacaag tttttcttgt cgaggctcat
       61 ggcaagacca acgactcgcc gcatgggcct acggctgcac ggtagaggtg gcagtgggag
      121 ttggagtcgg agtccgccag cgccaccacc atggggttgg agtcggccga ggagtattgg
      181 ttggagttgg ggtcggggtc tgatacggag tgggggtggc gatcgcagaa tccggataca
      241 gctggcgcat cgcagtcacc caatccatgt tttcgcgcag ggcaaactcg tcgaagcggg
      301 agccgggata gaagctcctc caattcgaga gcgcaggcag ccggtcgaat ccatagtctg
      361 cagctagggc cgtgaagtcg aggtagtagc cagccggcgg gttctcttgt tctgctccgc
      421 cgcggtcgta ggcagccggg ttaccggcgt agcgcagatc gaagtcccac ggacgccaac
      481 gaagcggctc gccctgcgaa ccatcttggg cagctgtgcg cacgaagatc cgccaatagg
      541 tctggagacc aaagtcctcc cgcaccaccg cgacccaacc ggcctgaaat gccgcccggc
      601 tgatcgcgaa ggctcggccg gtatacagcc aatcgttgta ggcatacccc ggcggcagcg
      661 gatcattgat cccgacgaat gcatgctcca actgggagag gaagtcccat ccggctgcct
      721 gagccgcccg cgctcgcaac tcgccaaaag cttcgtcggc tgcatcggat aagctcatcc
      781 cggacggacg aactccgtcg agcgggacga tcgccagccg ctcgccctct ggccgaggtt
      841 ggtcccagac aacctcgaag ggcgactcgg tagcggcctg agcgggcacc ccgagcccgg
      901 ccaagggaat cggtgaccag tgcaattggg aaatgcgccc ctccaatgac cctccgagct
      961 cgcccagcgc accttcgatc ggataggtca cgatatgtga ggtaaaggga gtgcgcaaga
     1021 ttgccgacaa cgcctgtccg tcagcgaccc aggccggatc ctgaccctgt ccaacccaga
     1081 cggcgctggc cgcaggctgg ccgagctgta gctgccgaat tccgtagacc ccatccagtt
     1141 ggatcgaata ggcgatctgg gaaccgtccg ggctgaaggc cggatctgcc tcggccaatt
     1201 ccaccgtgtg agtcagatta gtgaatcggt cgtcgggctt atcgaggtcg gccaggtaca
     1261 ggtctgggga cccttcccgg tctgatatga atgcaatcct ccggccacct ggatcccagg
     1321 aaggggcgac atcggccgcc gggtgatccg tgagttggac cggagactgg cttccatcaa
     1381 tggggagaat ccagatattg aagtcatccg cataatgggc ctcgtaggcc agccaaaggc
     1441 catcgggcga ccaggtcgga ttcccctcga atccgggcgt cccggtcagt cggcgaagtt
     1501 ccccggtcag caggtccaat aagtacagtt cccaattccc atctcgacgc gaactgaagg
     1561 ccagctggtc cagattgggg ctgaccgcag gatcacggtt gtcccaatct ccctcggtca
     1621 gctgacgaac ctggggatcg ccggggagga aggcgaggat ctggcttcgg ccagccacgc
     1681 tggccgacac cacgaaggcg cctgcgtggc tgataaggtt cgggcgggcc ggcgccgtag
     1741 cggctagctc tggtaccgcg gcgagctcct gcagcctcat gggcaccacc gggtcgagag
     1801 cagccggcgc ggcactcccg gcgctcagtc cagcccaacc tgccaggccg accagcgccg
     1861 ggatcgagac taggagaagt gcgacgcttc gccacccgcg gggcttacct cgggcagcgc
     1921 tacgccgtga ttttatgagc aagctgcagc ttccctgcgg cccattccgg ggccgcccgc
     1981 attatacaga cggagccatt gggtgcaagc cagatgccaa agaaggacaa gaaaaggccg
     2041 cctgcgggcg gccttttctg gacaggagat tgggagtcgt aaccaatccg gctggtcagg
     2101 ccgatacgat ggaggttacc agcgactttc ggccacctcg acggggagca ggaaggactc
     2161 ggcaaacagc tcttcactcc ccccctgcgc ccgataggca tcgacaaacg cgttcggtgc
     2221 taacaggatg gaggcgaagt aacttgccga ttccttcaat tccgacatta acaggtcggg
     2281 aagaccgatg tctctgccgt gcttgctcgt gatcagggcg cttagggttt ctcgggccag
     2341 ggcatgccgc ctggcggttt cgcccagatc gaccctcacg aaaatgctgc cacgttgacc
     2401 ggggggacga gcgaatgtcg catcccacag gttcgagtac atgtcgacca ccccgaaatc
     2461 ctcgtggaaa acttctgggg ggtgatcgag gatggcctcg atcggcactg gaggctctgc
     2521 ggcgccgcta tactcgacca gggcgcg
//
LOCUS       DATDPQ010000066         5921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3685628, whole genome shotgun sequence.
ACCESSION   DATDPQ010000066 DATDPQ010000000
VERSION     DATDPQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3685628"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..657)
                     /locus_tag="VI520_01510"
     CDS             complement(103..657)
                     /locus_tag="VI520_01510"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLF36590.1"
                     /translation="MPDYFSGTGDDGTTGLLGSQRVPKFHPRPAATGALDEAAAALGL
                     ARALAVELQTRELIVEIQRDLYHIMAEVAATRDQAHRFRELGADRVAWLEAEVRRIGD
                     QISMPKGFVIGGDSAPGAALDLARTIVRRAERAVVQLAHEGELNNPALLAYLNRLSSL
                     CFVLALWENRQAGVESPTLAKPEP"
     gene            682..1245
                     /locus_tag="VI520_01515"
     CDS             682..1245
                     /locus_tag="VI520_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HLF36591.1"
                     /translation="MDTPEPAAILRSSRQLLAGVLVGLLLGGVYYLLSAEPRGKPVEL
                     LPPQPLRIHVAGAVARPGVYSLPRGSIVQEAITAAGGLRGDADLARLNLAQPLEDGRR
                     IEVPLLVESVASSGVQLAGSEVRLPLNSATAPELEQLPGIGPVLAQKIIEYRDQFGPF
                     QRFEDLLQVEGIGPAKLEGLRDLLVVP"
     gene            1318..2145
                     /gene="mutM"
                     /locus_tag="VI520_01520"
     CDS             1318..2145
                     /gene="mutM"
                     /locus_tag="VI520_01520"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HLF36592.1"
                     /translation="MPELPEVETICRSLRDGTADAPSLVGEQIIGVRLGWPRHVVEPS
                     PTNFRQRIRRQSVRQIDRRGKFILLSLDRDSLLVHLMMSGDLFIAPAGARRTTHDHTI
                     FRLASGRQLRFRDPRKFGRVFLVADPETVLKRLGPEPLSAGFQVKKLQLILNRHKRAL
                     KPLLLDQTAIAGLGNIYADEALHRARLHPLRSSQTLQPSEVRALWKGIRAALRDGLRH
                     NGASIDWVYRGGDFQNHFRVYGQAGQPCPVCGTTIARSVIGQRSTFTCPNCQPWGKN"
     gene            2148..2840
                     /locus_tag="VI520_01525"
     CDS             2148..2840
                     /locus_tag="VI520_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36593.1"
                     /translation="MDQELLVGVLLIIVGLAVGLIAVALVLNLRTAREEQSADSPVPG
                     EAADDSAAEGAKAEVAELVATQSDEGPVAMSVPDEQRPAPAGLEERKLVAEIYRDPTG
                     RGLILRAGGKDYRAISQIEDEQLRMELTTLAADLASRFVEGLRAPAGPSAPRTAARAG
                     SRSMLEGINSILQRVLADAGNKAKGVQLIPDAAGGVKVLIGLKSYALDEVPDEEISRL
                     IRESVAEWEAGQ"
     gene            complement(2791..3690)
                     /locus_tag="VI520_01530"
     CDS             complement(2791..3690)
                     /locus_tag="VI520_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF36594.1"
                     /translation="MYRGELEFHLVSDGIVWVDPGGPFGLVPRALYRRIVEPNQNHLI
                     PQTLTCMLVRSEGKTILIDTGLGPKLDPKDEKNWGLMRAGGGLVNNLEALGISPSDID
                     LVICTHLHADHCGGNTRLEAGKLVPTFPNATYLVQRMEWADASHPDPRTRGTYFAENF
                     SPLVSEGRMRLLHGDQPITRHVRCAVTPGHTRGHQSVVLESGGWSGLFVSDMATFSVH
                     MARTGWLTAFDVEPLENIRTKQRWQRWALEREAWLFFIHDPIKPVAQLREQDGRLEAL
                     LVEEARPVIDPLPTPQPILESGG"
     gene            complement(3677..4699)
                     /gene="add"
                     /locus_tag="VI520_01535"
     CDS             complement(3677..4699)
                     /gene="add"
                     /locus_tag="VI520_01535"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HLF36595.1"
                     /translation="MPKVDLHRHLEGSLRLKTLLEVARQEDLDLPQEEAELRERVQIA
                     GEQPKTLPNFLSKFRTLRQIYRSPEIIRRVTREAIEDAAEDQVRYLELRFTPVALSQA
                     KDFPLAEVVDWVIEFATEAAEQVGVRVGLIASVNRHEPIRLAERVVELAVARMGDGIA
                     GLDLAGNEGEYPAEPFVSLFRAARAAGLGITVHAGEWAGAGSVIQAVNEMQASRIGHG
                     VRVMEDPQAVQVARAARTVFEVCLTSNLHSGAVSDMGNHPLPQMIDSGLQVTLNTDDP
                     GISNIRLSDEYYTAVAELGLSIESLKGLILAAGQAAFVSDRGKRELERELKQALGLAS
                     VPDVPG"
     gene            complement(4755..>5921)
                     /locus_tag="VI520_01540"
     CDS             complement(4755..>5921)
                     /locus_tag="VI520_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36596.1"
                     /translation="ADREQPYPSFMLKLLAERLESIDQADELGSALARLAEDSLQAQA
                     EHLNRAAQIQLERALSSLETQVRERTDLAEMAIDGAYLEAEQSEAPIDPSALLSAVES
                     TAGLRLEANGKVRQLIRDDPNRFRASVPELIEASLGMRVWAGLIQTLENRVGESLNLQ
                     LQEPIDWDRAEAELFSALTRVQERRAAQAVVDISRELETLLQGGKSVEGPLKTRALLQ
                     LSYARQTLFDSRSHQAREVIIPRLSYVYHAASVLKGRHPVELNEAILAHLEGAREYLK
                     QAIGSSTLAQIHRQLFLGVGDRLWVDYLTQMEALRTSIGLEAYGQRDPLVQYKSRAVD
                     LFRALLGNIRAGIVGRLFRIQFGTPQPAVAARPAVAQPAPSPGTKSRRRRRRGR"
BASE COUNT         1071 a         1878 c         1893 g         1079 t
ORIGIN      
        1 cgagttagcg gaagcggtaa cctggatgac acgggaggaa gtcccgatcc gcgaagtgga
       61 ctgccgggca agtggtaaag aagcgtgccg cttcgcggtc ctctaaggtt cgggcttagc
      121 cagggtcggg gattccacac ccgcctgccg attctcccac agggcgagca cgaagcacag
      181 cgacgacaac cggttgaggt aggccagcag ggcgggattg ttcaactccc cctcgtgagc
      241 gagttgaacc accgcccgtt cggcccgccg gacaatcgtt cgggccagat ccagggccgc
      301 gccgggcgcg gaatcgcctc cgatcacgaa accctttggc atcgagattt gatccccgat
      361 ccgcctaact tcagcctcca gccaggcgac ccggtccgct ccgagctccc gaaaccggtg
      421 ggcctgatcg cgggttgccg caacttcggc catgatgtgg tagagatccc gctggatctc
      481 gacaatcagt tcccgggtct gtagctcaac ggctaaggcc cgggccaacc cgagcgccgc
      541 ggcagcctca tcgagcgcgc cggttgcggc aggccgtgga tggaacttag ggacccgttg
      601 gctgccgagc aatccggtgg ttccatcatc gccggtgccc gaaaagtagt ctggcatccc
      661 aggtattata agtagaagcg cgtggacaca cccgaaccgg ccgcaatcct tcgaagctct
      721 cgccagcttc ttgctggagt cctggtcggg ttactactgg ggggcgttta ctacctgctg
      781 agtgcggagc cgagagggaa gccggtcgaa ctgctgcccc cacagccctt gcgaatccac
      841 gtagccggag ctgttgcgcg gcccggcgtc tattctctcc cgcgcggatc gatcgttcaa
      901 gaggcgatca ccgcggctgg aggactgcgc ggcgacgcgg acctcgcccg gttgaatctg
      961 gcccagcctc ttgaagacgg tcggcggatc gaggtcccgc tgctggtcga atccgtggcc
     1021 agctctgggg tccagttggc gggcagcgag gtccgtctgc cactcaattc cgccaccgca
     1081 ccggagctgg agcagcttcc cgggatcggc ccggtcttgg cccaaaagat catcgaatac
     1141 cgggatcaat tcggtccgtt tcaacggttc gaggatctgc tccaggtcga agggatcggc
     1201 ccggcaaagc ttgaaggcct gcgggatttg ctcgtcgtcc cttaaccagc cgaccccggc
     1261 cggcggagga cacgggcgag gcgaggcttc gccgaccccg ctgtataatc cgcacagatg
     1321 cctgaacttc cggaggtcga gaccatctgc cgatcgttac gtgacggcac ggcggatgct
     1381 ccgtccctgg tcggcgagca gatcatcggg gtccggttgg gctggccccg ccatgttgtg
     1441 gagccctcgc cgacgaactt tcggcagcgg atccgacggc aaagcgttcg gcagatcgat
     1501 cgccggggta agttcatcct cctctcgctg gaccgcgatt cgcttctggt ccaccttatg
     1561 atgagcggcg acctcttcat cgctccggcc ggagctcgcc ggacgaccca cgatcacaca
     1621 atcttccggc tcgcctcggg gcggcagctg cggttccgcg acccccggaa gttcggtcgg
     1681 gtctttctcg tggcggaccc cgaaaccgtg ctcaagcggc ttggtccgga accgctgtcg
     1741 gctgggttcc aagtgaagaa gctccagctg attctcaatc ggcacaaacg agctctgaag
     1801 ccgctgctct tggatcagac ggcgatcgcc ggactgggca atatctatgc ggacgaggct
     1861 cttcatcggg ccaggctcca cccgctccgc agcagtcaga cgctgcagcc ctccgaagtc
     1921 cgtgcgctct ggaaggggat ccgcgccgca ctgcgggatg ggctgcgtca caatggtgcg
     1981 agcattgatt gggtgtatcg gggtggggat tttcagaacc actttcgggt ctacgggcag
     2041 gcagggcagc catgcccggt ctgcggaacg acaatagccc ggtcggtgat tggccagcgc
     2101 agcaccttta cctgcccgaa ctgccagccc tggggtaaga actaatcatg gatcaggagt
     2161 tgctggtcgg ggttctgttg atcatcgtcg gcctcgccgt cgggttgatc gcggtcgcgt
     2221 tggtcctgaa tcttcggact gcccgggaag agcagtcggc cgactcaccc gtgccggggg
     2281 aggctgcgga tgactccgcg gccgaaggcg ccaaggccga agtcgcggaa cttgtggcga
     2341 ctcagagtga cgaagggccg gtggcgatga gtgtgccgga tgagcagaga ccggcgcccg
     2401 ccggcctcga ggaacggaag ctcgttgccg agatttaccg ggaccccacc ggcagagggc
     2461 tgatcttgcg ggcaggcggc aaggattacc gagcaatctc tcagatcgaa gacgagcagc
     2521 tccggatgga gctgactacc ctggccgccg atcttgcaag tcgattcgtg gagggcctgc
     2581 gtgccccagc cggaccgtcc gccccgcgga ccgccgcccg ggcaggcagc cggagtatgt
     2641 tggaaggcat caactctatc ttgcagcggg tgctcgccga tgcaggcaat aaggccaagg
     2701 gcgtgcaact gatccccgat gcggcaggtg gggtcaaggt gttgatcgga ttgaagagct
     2761 atgccttaga tgaggtgcca gacgaagaga tcagccgcct gattcgagaa tcggttgcgg
     2821 agtgggaagc gggtcaataa ccggccgggc ttcttcaaca agaagcgctt ccagtcgccc
     2881 gtcctgttcg cgcagttggg cgaccggctt aatcggatcg tggatgaaga aaagccaggc
     2941 ctctcgttcc aaggcccagc gctgccagcg ctgcttggtc ctgatgttct caagtggctc
     3001 tacatcaaag gcagtcagcc agccggtccg ggccatgtga actgaaaacg tagccatatc
     3061 ggaaacgaac aggccggacc agcctcccga ctccagcacc accgactggt ggcctcgggt
     3121 atgccccggg gtcaccgcac agcggacatg ccgggtgatc ggctgatcac cgtgcagtaa
     3181 gcgcatccgc ccttccgaaa ccagcgggct gaagttctcc gcgaagtacg tccctcgggt
     3241 gcgcgggtcg gggtggctgg cgtccgccca ttccatgcgt tgaacaagat aggtcgcgtt
     3301 cggaaaggtg ggcacgagct tgccggcttc gaggcgggtg ttcccgccgc agtggtcggc
     3361 atgcagatgg gtgcagatca ccagatcaat atcggacggc gagatcccaa gagcctccag
     3421 gttgttgacc agcccgccgc cggcccgcat cagtccccag ttcttctcgt ctttcgggtc
     3481 cagcttaggg cccaaaccgg tatcgatcaa gatcgtcttg ccttccgacc ggaccagcat
     3541 gcaggtcaga gtctggggaa tcaggtggtt ttggttcggt tcaacgattc ggcggtagag
     3601 ggcccggggc accagcccga acggaccgcc ggggtcgacc cagacgatcc cgtcacttac
     3661 caggtggaac tccagctcac cccggtacat cggggaccga ggcgagtccg agcgcctgct
     3721 tcagctcgcg ctccaattcg cgtttcccgc ggtccgaaac aaaggcggcc tgaccggcgg
     3781 ccaggatcag ccccttgagg ctctcaatcg agagaccgag ttcagcgacc gccgtgtaat
     3841 actcgtcgct cagtcgaatg ttggagattc cggggtcgtc ggtgtttaga gtgacctgca
     3901 aacccgaatc gatcatctgc ggcagcgggt gattccccat gtcggaaacg gcgcccgaat
     3961 gcaggttgct ggtcagacaa acttcgaaca ccgtccgggc ggctcgcgcc acctgcaccg
     4021 cctgcgggtc ttccattacc cggaccccgt ggccgatccg cgaagcctgc atctcgttca
     4081 ccgcctggat gactgagccg gcacctgccc actctccggc gtgcacggtg attccgagcc
     4141 cggcagcccg cgcagctctg aatagggata cgaaaggctc ggccggatac tcaccctcgt
     4201 ttccagccag atccagtcca gcgatcccgt cccccattcg agccacggcc aactcgacca
     4261 cccgctccgc cagccgaatg ggttcgtggc ggttgacgct agcaatcaaa ccgactcgaa
     4321 ctccaacctg ttcggcagcc tcggtagcaa actcgatcac ccaatcgacg acctcggcga
     4381 gtgggaaatc ctttgcctgc gagagagcca ccggcgtgaa gcgcagctcc aagtagcgga
     4441 cttggtcttc ggccgcatct tcgattgcct cccgagtcac ccggcggatg atctcaggcg
     4501 accggtagat ctggcgcaag gtgcggaatt tggagaggaa gttcgggaga gtcttgggct
     4561 gctctcccgc gatttggacc cgctccctga gctcggcttc ctcttgaggc aggtcgagat
     4621 cctcttgccg ggcgacctcg agcaaggtct tgagccgcag ggagccctcc agatgccggt
     4681 gcaggtcaac cttgggcaag accgcgagct cttgctgaag ctcgaacggt gagctattag
     4741 gcggcacggg cggctcagcg accccgacgc cgtcggcgcc gagacttggt gcccgggctc
     4801 ggagccggtt gggccactgc gggtcgggcc gcgaccgccg gctggggtgt cccaaattgg
     4861 atcctgaata ggcggcccac aattccggcc cggatatttc cgagcagcgc ccggaaaaga
     4921 tcgaccgccc ggctcttgta ctggaccagc gggtcccgct ggccataggc ttcgagtccg
     4981 atcgaggtcc gcaaggcctc catctgggtc agataatcga cccacaagcg gtcgccgacc
     5041 cccaggaaca attgccgatg gatctgagcg agggtgctgc tgccgattgc ctgcttcagg
     5101 tactcgcggg ccccttcgag gtgggccagg atcgcctcat tcagttccac cggatggcga
     5161 cccttcagca cgctcgcggc gtggtagaca taggagagcc gcgggatgat gacttccctg
     5221 gcctggtggc tccgcgaatc gaacagggtc tgccgggcgt agctgagctg aagcagggcg
     5281 cgggtcttaa gtggaccctc aaccgacttg ccgccctgca gcagggtttc gagctcgcga
     5341 gaaatgtcaa ccaccgcctg ggctgcccgg cgctcctgca cccgagtcaa ggccgagaac
     5401 agctcggctt cagcccggtc ccaatcaatt ggttcttgca gctgtaaatt gagactctcg
     5461 ccgactcgat tctccaaggt ctggatcaga ccggcccaga cccgcatccc caggctggcc
     5521 tcaatcaatt cggggaccga ggcccggaat cggttcgggt catcccggat cagttgtcgg
     5581 acctttccgt tggcctccaa ccgtaatccg gcggttgact ccacggcgct caagagcgcg
     5641 ctcgggtcga tcggagcttc agactgttcg gcctccaggt atgcgccgtc gatagccatc
     5701 tcggccaagt cggtccgctc ccggacctgg gtttccaagc tcgagagggc ccgctcgagc
     5761 tgaatctggg cggctcgatt gaggtgctcc gcttgggctt gaagcgagtc ttcggccagc
     5821 ctggccaacg ccgagccaag ctcgtcggct tgatcgatcg attccagccg ctcggcaagc
     5881 agcttcagca tgaaggaggg atacggctgc tcgcggtccg c
//
LOCUS       DATDPQ010000067         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1073018, whole genome shotgun sequence.
ACCESSION   DATDPQ010000067 DATDPQ010000000
VERSION     DATDPQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073018"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VI520_01545"
     CDS             complement(<1..1379)
                     /locus_tag="VI520_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF36597.1"
                     /translation="MVVQVQELKPDTREIWSAALASAAGVARQASASEEDLLRAVTKE
                     LRRLHLHGGLALLQPDGQMAVGAITYGSLIERQLSRLFGGAIVGFRFDPEPVDLIRHT
                     LETQDSIFTSDRAELLEQALPERLHSLVAPILSVLAKHPFIIAPLVLDGRALGILTVT
                     APWLTPEDRLMVAALADHVAIGLGHVRVRAEMRHALERERLRNQVIEAVASSLDLSDV
                     LKKVLDMAIEVVDAEAGAIALVDPDGEKLTYPYLVGLPGSLRQEVTPKGEGLAWRLIE
                     TGKPLLLHEYSHHPSALPSWVEAGIRAYLGVPLIAGDQPIGGLGLFNKRKGRTFTDEQ
                     VERAQAVATMAAIAVQHAWLLAQANERAEEAQALIQTARSISASLDQQTVLKLIAEKA
                     KDLLLADGSRIHLHDPQTNSLRCVIALDEPAPAFQALDLKPGEGLVGHVVESGKPLIS
                     NDPPGDPRGL"
     gene            1499..2500
                     /locus_tag="VI520_01550"
     CDS             1499..2500
                     /locus_tag="VI520_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HLF36598.1"
                     /translation="MSLLANWKEVMDTANLSAGKQMDVVSKWLIITRAAVFTMTATSG
                     MIGGLLAAAVAENPNWLNFGLAFVGLVIAHAANNMNNDYFDMAGGVDTDDYARAQYAP
                     HPVLSGMLTKRGLGGAVVILNAIDLLILIALYVRTDWPVIAFALAGLFISVFYVAPPL
                     KLKHHGLGEPGVFLVWGPLMIGGTYYVTAGALPSAGVWLATIPYAVAVATVLMGKHID
                     KREADKAKGIHTLPVLIGERASLRLNMALMVAFYLIVVALVLTGTLGVWVLLVFLAVP
                     RLKTALEIYRKPKPTQPPENYPVWPLWFVSWAFYHNKRAGFLFVIGLVINLLIPVSL"
BASE COUNT          454 a          755 c          800 g          501 t
ORIGIN      
        1 aggccccgcg gatcgccagg tgggtcgttg ctgatgagcg gcttgccgct ctcgacgacg
       61 tggccgacca ggccttcgcc gggcttcaaa tcgagagcct gaaatgcggg ggcgggctcg
      121 tcgagcgcga tcacacagcg caggctgttg gtctggggat cgtgcaggtg aatccggctg
      181 ccgtccgcca gcagcaagtc cttggccttt tccgcgatca gcttgagcac cgtctgctgg
      241 tcgagggagg ccgagattga gcgggcggtt tggatcagtg cctgggcttc ttctgcccgc
      301 tcgttggcct gggcgagcaa ccaggcgtgt tggaccgcga tggcggccat ggtggcaacc
      361 gcctgggctc gctcgacctg ttcatcggta aacgttcgcc cctttcgttt attgaagaga
      421 cccaggccac cgatgggttg atcgccagca atcagcggca cccctaaata ggcccggatt
      481 ccggcttcga cccatgacgg gagggcgctc ggatggtgag agtactcgtg cagcagcagc
      541 ggcttgccgg tctcgattaa tcgccaggct agaccttcgc ctttcggggt gacctcctgc
      601 cggagcgagc cgggcaggcc caccaggtag ggatacgtca gcttctcacc gtcggggtcg
      661 accaaggcaa tcgctccagc ctccgcgtcg actacctcga tggccatatc caggaccttc
      721 ttgagtacgt ccgacaggtc gagcgagctg gcgaccgctt cgataacctg attgcgcagc
      781 cgctcccgct ccagcgcgtg ccgcatctct gcccgaaccc ggacgtggcc taatccaatc
      841 gccacgtggt cggcgagggc ggccaccatc agccggtctt ccggggtcaa ccagggggcg
      901 gtcacagtca ggatacccag cgcacgaccg tcgaggacca acggtgcgat gatgaaaggg
      961 tgcttggcca ggacagaaag gattggtgcc accagtgagt gcaggcgttc aggcaatgcc
     1021 tgttccagca gctctgcccg gtctgaggtg aagatcgagt cttgggtctc gagtgtgtgg
     1081 cgaatgagat cgactggctc cgggtcaaag cggaatccaa cgatcgcccc accgaacaat
     1141 cggctcaact gccgctcgat cagggagccg tacgtgattg cacccacggc catctgcccg
     1201 tcgggctgca agagcgcaag cccgccgtgc aaatgaagcc gacggagctc tttggtaacg
     1261 gcccgcaaca ggtcttcttc gctagccgac gcctggcgcg ccactccggc ggcagacgcg
     1321 agggccgccg accatatttc tcgggtgtct ggcttcagtt cctggacctg gacgaccact
     1381 ggcagggcct cgagggggcg gcgagtctag catggaacca gccgcgccgc aggtgccttt
     1441 acacggtaat cccaaggcgt tagactcgcc ctgagctatt agtcccatag gagcgaggat
     1501 gtccctgctg gcgaattgga aggaagtaat ggataccgcc aacctgtctg ccggcaagca
     1561 gatggacgtg gtcagcaagt ggttgatcat cacccgggcg gctgtgttca cgatgacggc
     1621 aacctccggc atgatcggag ggctgctggc cgccgcggtg gccgaaaacc caaactggct
     1681 taacttcggt ctggcgtttg ttgggcttgt catcgcccac gctgccaaca acatgaataa
     1741 cgactacttc gatatggcag gcggggtaga caccgacgac tatgcccggg cgcagtacgc
     1801 ccctcacccg gtgctttccg ggatgctgac caaacgcggg ttgggcggtg cggttgtgat
     1861 tctcaatgcc atcgacctac tgatcctgat cgcactatat gtacgaaccg attggccggt
     1921 gattgccttc gccctggcgg gtctgtttat cagcgtcttt tacgtcgcgc ccccgctgaa
     1981 gctcaaacac cacgggctgg gggagccggg ggtgttcttg gtatggggac ccttgatgat
     2041 cggagggacc tactatgtca cggcgggtgc gcttccgtcg gccggagtgt ggctggcaac
     2101 cattccttat gcagtagcgg tagctacggt gctgatgggc aagcacatcg ataagcggga
     2161 ggccgacaag gcaaaaggca tccacaccct cccggtgctc atcggagagc gagcctctct
     2221 acgcctcaac atggccttga tggtggcctt ctatctaatc gtggtggccc tagtgctgac
     2281 cggcaccctc ggggtttggg tgctgctggt gttcctcgcc gtcccacggt tgaagaccgc
     2341 tctggagatc taccgcaaac ccaagcctac tcagcccccg gagaactatc cggtctggcc
     2401 gctgtggttc gtgtcatggg ccttttatca caacaagcgg gccgggtttc tgttcgtaat
     2461 cggactggtg atcaatctgc tgattccggt gagcttgtaa gattcggagc
//
LOCUS       DATDPQ010000068         4594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_284944, whole genome shotgun sequence.
ACCESSION   DATDPQ010000068 DATDPQ010000000
VERSION     DATDPQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284944"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VI520_01555"
     CDS             <1..993
                     /locus_tag="VI520_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HLF36599.1"
                     /translation="PASLPTRSMAISRFRVLARDRLAAYSNFPWSLGVALGMLVFFIP
                     HPEWHLDLQWFRDQTDILHYPAYAHWIFALLGWLPEPVAYVGLSLATTAGLYLAVRAF
                     GGAHWVVFTSFAFAWILFYGQLDGLVAGGLGLAWWASGRNRPVLAGAGLILACLKPQL
                     SLPASLALWWWSPSRLKSLLVPVAVLLLSLVTSGFWIPVWLDRLGDSDQWVLLSRNLS
                     LWPQVGNWIFLIWPFILALPGSKERKLVAVLAGTALTSPYFPLPSAVLILAMGVPWWA
                     WGIVQLPMLSFLVGSWIYFVGKALPVTLLLWALWPGLRNVLRRPKPQDRSRSHV"
     gene            986..2494
                     /gene="accC"
                     /locus_tag="VI520_01560"
     CDS             986..2494
                     /gene="accC"
                     /locus_tag="VI520_01560"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HLF36600.1"
                     /translation="MFNKVLIANRGEIAVRIIRACRELGLKTVAVYSEVDRSALHVRQ
                     SDEAYLLGPPASRDSYLRGDKIIEIAKRSGVGAIHPGYGFLAERDDFAQAVQEAGLIF
                     IGPRPSAIAAMGDKAVARATVAAAGIPVVPGTEGEGSLRDEELLAIAPQIGFPLLIKA
                     TAGGGGKGMREVDQLQEMPGLLAAARREAEAAFGDGNVYLEKLISEARHIEFQIMADS
                     YGNVIHLGERECSLQRRHQKLLEESPSPFMDDELRSRMGELACRAARSVDYLNAGTIE
                     FLVDADRNFYFLEMNTRLQVEHPVTEMVTGVDIVREQIRIARGRRLRYSQDQIQLNGW
                     ALECRINAEDPHNNFRPSTGQLTSVIPPSGPGVRVDTGVYAGFTITPYYDSLISKLIC
                     WGETRGEAILRMRRALEEFRILGVRTNIPFHQQLLDSHRFIGGQFDTRFVEERFQLEQ
                     PGQVEGKMPLIAAVLATLVTHRETQLAAQIVRRSERDTSNWKWVGRWERMHR"
     gene            2561..3058
                     /locus_tag="VI520_01565"
     CDS             2561..3058
                     /locus_tag="VI520_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HLF36601.1"
                     /translation="MKYLATIGDRTFEIEINADGQILADGQLLAVDFHSVADQPVYSL
                     LLDGESFEANVSSADQNIEVLLRGHLFVVTVEDERQRRLRESTSAGPIQEGEYSLTAP
                     MPGLVVAIPVEEGQPVEHGQNLVILESMKMQNELKAPRAGTVGRIRVRTGDNVEQNQT
                     LLSLT"
     gene            3055..3675
                     /locus_tag="VI520_01570"
     CDS             3055..3675
                     /locus_tag="VI520_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HLF36602.1"
                     /translation="MKEIEAKFYVSRLELIRSRVTRLGGRLRARQHLERNWGYDLPGD
                     PLAKQLSLLRLRQDRAATLTYKRRGRSIESREEIELQVADPSTADALLAALGYRVVAS
                     YEKLRREYSLGPVRVMLDELPFGEFVEIEGPGLQLIKRAAFQLGLVWSRRVRNTYLEL
                     FDQLRAEYSLRFREASFERFATLPQIRADRIGLRDAVRPELPARDR"
     gene            3665..>4594
                     /locus_tag="VI520_01575"
     CDS             3665..>4594
                     /locus_tag="VI520_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLF36603.1"
                     /translation="MTDELKSASQRWEAEVVAQASKDLPERQSHFETPSQIGLQRVYL
                     PEHSAADYLEKVGFPGQEPYTRGVHPTMYRGRLWTMRQYAGFASARETNQRFRYLLEQ
                     GQSGLSVAFDLPTQIGYDADDPLAAGEVGRVGVSISSLDDLELLFQGIPLDQVSTSMT
                     INAPAAILLAMYVAVGQRHNIDPGRLRGTTQNDILKEYVARGTYIYPPGPAMRLVTDV
                     IGYCRRVATQWNPISISGYHMREAGCTAVQEVAFTFANALAYVEAALAAGMQVDEFAP
                     RLTFFFSAHNDLLEEVAKFRAARRLWPRLISKRF"
BASE COUNT          806 a         1429 c         1470 g          889 t
ORIGIN      
        1 cccgcttcgc tgccgaccag aagcatggcg atatcccgct ttagggtgct cgcccgggac
       61 cggctcgccg cctactcgaa cttcccgtgg tcgttggggg tcgccctcgg gatgctggtg
      121 ttcttcatcc cccaccccga atggcacctg gacctgcaat ggttccgcga ccagacagat
      181 atcctgcact acccggcgta cgctcactgg atcttcgcgc tgctggggtg gctaccggaa
      241 ccggtggcct atgtggggct ttccctggcc acgactgccg ggctctacct ggcagtgcgg
      301 gccttcggcg gggcgcactg ggtggtcttc acctcgttcg ccttcgcctg gatcctcttc
      361 tacgggcaac ttgacggtct cgttgcgggc ggcttggggc tagcctggtg ggcctccggg
      421 cgaaatcggc cggtgctggc gggggcggga ctcatcctgg cctgtctcaa accgcagctt
      481 tccctgccag cctccctcgc tctctggtgg tggtcgcctt cccgactcaa gtcgctgctc
      541 gtgcccgtgg cggtcttgct gctctcctta gtgacgtcgg gattctggat tccggtgtgg
      601 ctcgacaggc tgggcgacag cgaccaatgg gtcctgctgt cccgcaacct ttcgctgtgg
      661 ccgcaggtcg gcaattggat cttcctcatc tggcctttca tcctcgccct cccagggtcg
      721 aaggagcgca agcttgttgc ggttctggcc ggcactgcgc ttaccagccc ttacttccca
      781 cttccttcgg cggtgcttat cctggcgatg ggcgtgccct ggtgggcatg gggtatcgtt
      841 cagctgccca tgttaagctt tctagtgggg tcgtggatat acttcgtagg caaggcgctg
      901 ccggtgaccc tgctgctctg ggcgctgtgg ccagggctgc gaaacgtgct tcgccgccct
      961 aagccgcagg atcggagtcg gtcgcatgtt taataaggtc ttgatcgcca accggggtga
     1021 gatcgcagtt cgcatcatcc gagcctgccg cgagctgggc ctcaaaaccg tggcggttta
     1081 ctccgaggtc gatcggtccg cgctgcatgt acgccagtcc gacgaggcct acctgcttgg
     1141 gccgccggct tcccgcgact cctatctgcg cggcgacaag atcatcgaaa ttgccaagcg
     1201 atccggggtc ggtgcgattc accccggcta tggattcttg gcggagcggg atgatttcgc
     1261 tcaggcggtt caggaggcag ggctgatctt catcggtccc cgcccgtccg cgatcgcggc
     1321 catgggcgat aaggccgtcg cccgggcaac cgtggcggcc gccggaattc cggtcgtccc
     1381 cggtactgaa ggagagggca gtctgcggga cgaggaactg ctggcgatcg cgccccagat
     1441 cggattccca ctgttgatca aagccaccgc cggaggcggg ggcaagggaa tgcgggaggt
     1501 ggatcagctg caggagatgc ccgggctgct ggccgcggcc cgccgggagg cagaggccgc
     1561 gtttggggat gggaacgtct atctggaaaa gttgatctcg gaggcccgcc acatcgagtt
     1621 tcagatcatg gccgacagct atggcaacgt gatccatcta ggcgaacgcg agtgttcgct
     1681 ccagcgccgc caccagaagc tgctggaaga atccccctcc cccttcatgg acgatgagct
     1741 gcggagtcgg atgggtgagc tggcctgccg ggcagcgcgt tcggtcgact acctgaatgc
     1801 tgggaccatc gaattcctgg tggacgccga tcgcaacttc tatttcctgg agatgaacac
     1861 ccggctgcag gtcgaacacc cggtcaccga gatggtcacc ggagtggata tcgtccggga
     1921 acagatccga attgctcgag ggcggcgatt gcgctacagc caggatcaaa tccagctcaa
     1981 cggctgggcg ctcgagtgcc ggatcaatgc cgaagatccg cacaacaact ttcgcccgag
     2041 caccggccag ctcacctcgg taattccacc cagcggccca ggggtccggg tggataccgg
     2101 cgtgtacgcc ggcttcacga ttacgcctta ctacgactcc ctgatctcca agctaatctg
     2161 ctggggcgaa actcggggcg aggccatcct gcgaatgcgc cgggcactgg aagagttccg
     2221 aattctggga gtccggacca acatcccatt tcaccagcaa ctgctcgact cgcaccgctt
     2281 tatcggcggt cagtttgaca cccggttcgt agaagagcgt ttccagctcg aacagccggg
     2341 gcaggtcgag ggcaagatgc ccctgatcgc ggcggtactg gccactctag tcacccaccg
     2401 tgagacccaa ctggctgccc aaatcgtgcg ccggagcgaa cgcgacacga gcaactggaa
     2461 gtgggtgggg cgctgggaac ggatgcatcg ctgagattgc tgagcccatg cagccaccct
     2521 cgccagccaa actcggccca cctgttcatg gatcgccccg atgaagtacc tcgcaacgat
     2581 tggagaccgg acttttgaaa tcgagatcaa cgccgacggc cagatcctgg cggatgggca
     2641 attgctggcg gtggactttc actcggtggc cgaccagccg gtctactctc ttctgctgga
     2701 cggagagtcg tttgaagcca atgtctcgag cgcagaccag aacatcgaag tgctgctgcg
     2761 cggccatctc ttcgtcgtca ctgtcgaaga cgaacgccaa cggcggctgc gggagagcac
     2821 ctcggccgga ccgattcagg aaggcgagta cagcctgacg gctccgatgc cgggcctggt
     2881 ggttgctatt ccagttgaag aaggtcagcc agtcgagcac ggacaaaacc tggtgatcct
     2941 ggagtcgatg aagatgcaaa acgagctcaa ggcgccccgg gcggggaccg tgggccggat
     3001 ccgggtgcgg accggggaca atgtcgagca gaatcagact ctgctcagct tgacgtgaag
     3061 gagattgaag ccaagtttta cgtcagccgg ctggagctca tccgcagccg ggtcactcga
     3121 ttgggaggcc ggctgcgtgc tcgccaacat ttggaacgca actggggtta cgacctgccc
     3181 ggcgatccac tggcaaagca gctctccctc ctgcgtctga gacaggatcg agcggcgacc
     3241 ctgacctaca agcggcgggg aagatcgatt gagagccggg aggagatcga gcttcaggtt
     3301 gccgatccct cgactgcaga tgcgctcttg gccgccctcg gctaccgggt tgtggcaagc
     3361 tatgagaagc tccggcggga gtactcgctg gggccggtcc gggtcatgtt ggatgagctc
     3421 ccgtttggtg aattcgtaga gatcgagggc ccaggattgc aactgatcaa gcgggcggcc
     3481 ttccaactcg gtctggtctg gagccggcgg gttcgcaaca cctatttgga gctcttcgac
     3541 cagctgcgcg ccgagtattc gctgcgtttt cgggaggcca gtttcgagcg cttcgcgacc
     3601 ctcccgcaaa ttcgggccga tcggatcgga ctgcgtgacg ccgtcaggcc ggagctgcca
     3661 gcccgtgacc gatgagctga agtcggcctc ccagcgctgg gaagcggagg tcgtcgccca
     3721 agccagcaaa gatctgccgg aacggcagag ccatttcgag actccctccc agatcggcct
     3781 gcagcgggtc tacctgcccg aacactctgc ggctgactac ctggagaagg tcggcttccc
     3841 aggccaggaa ccgtacaccc gcggcgttca tcccacgatg taccgcggcc ggctctggac
     3901 gatgcgtcag tacgctgggt tcgccagtgc ccgggagact aaccaacgct tccgctatct
     3961 gctagagcag ggtcagagcg gactgtccgt cgcgttcgac ctccccactc agatcggtta
     4021 cgatgccgat gacccactcg ccgccgggga ggtcgggaga gtcggggtgt ctatcagctc
     4081 cctggacgat ctggaactgc tgttccaggg aatcccgctg gatcaagtgt ccaccagcat
     4141 gactatcaac gcgccggccg ccattctcct ggcgatgtac gtcgcagtgg gccaacgcca
     4201 taacatcgac cctggccggc tgcgcggcac gacccagaac gacattctga aggagtacgt
     4261 cgcccgcggg acctacatct atccaccggg gcccgcaatg cggctggtca cggatgtgat
     4321 cggctactgc cgccgggttg cgacacagtg gaacccgatc tcaatctcgg ggtaccacat
     4381 gcgggaggcc ggctgcacgg ctgtgcagga ggtcgccttc accttcgcca acgccctggc
     4441 ctatgttgag gctgcgctgg cggccggaat gcaggtcgac gagttcgctc cgcggctgac
     4501 tttctttttc agtgcgcaca atgatctctt ggaagaggtc gccaaatttc gggccgcccg
     4561 ccggctgtgg cccaggctga tcagtaagcg attc
//
LOCUS       DATDPQ010000069         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3165960, whole genome shotgun sequence.
ACCESSION   DATDPQ010000069 DATDPQ010000000
VERSION     DATDPQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165960"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VI520_01580"
     CDS             <1..628
                     /locus_tag="VI520_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLF36604.1"
                     /translation="HGGRCEACQGQGQLRIEMQFLPDVYVPCEVCNGARFNRETLQVR
                     FKEKSIAEVLETTIDEGAGFFAAFPAMLRKIETLRDVGLGYLKIGQPATTLSGGEAQR
                     VKLSRELSKRGPGHTVYVLDEPSVGLHAADVHKLIEVLQQLVDQDNTVLIIEHHPDII
                     KVADWVIDLGPEGGDRGGRLLAEGTPEQISRVKASYTGQYLKPHLAKA"
     gene            768..1943
                     /locus_tag="VI520_01585"
     CDS             768..1943
                     /locus_tag="VI520_01585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF36605.1"
                     /translation="MPTGFDVIVIGAGIMGCSSAFQLAGRGMKVGVFDKRGLGAGSTG
                     KSSAIIRQHYSNEVTARMALHSLRVFQNFGDAVGGEAGFRRAGFLVLTAEKDVEGLRA
                     NVALQQRVGIKTDILSEAEVRRRWPFLKTNDLVAAAHEPDSGYADPNLTTGSYANAAQ
                     AKGAEFLLDTEVIGLQLRSGKVRGVTTSSGTFEAPVVVNCAGPWGARVARWAGIEVPI
                     SSCRVQVAFFRRPDDYADEHPVVADFTTASYWRPETGGLTLVGLIDPSELNHVVDPDS
                     YNERVELDFVADAGDRLVRRFPAMENSASASGYAGLYAITPDWHPVIDELIEGSGFYL
                     CSGFSGHGFKLGPAVGSMVADMVSGAITPGMDRRLFRLNRFAENEPVRGRYEYSIAG"
     gene            complement(1946..>2251)
                     /locus_tag="VI520_01590"
     CDS             complement(1946..>2251)
                     /locus_tag="VI520_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLF36606.1"
                     /translation="VTERTREIGLRKAVGARRRDILLQFLLESVVLAVAGGLLGVGLG
                     GLGTWGIGRAASDLAPTLSLQAVLLATSFSGLVGLFFGIYPATRAASLNPIDALRYE"
BASE COUNT          434 a          689 c          739 g          389 t
ORIGIN      
        1 ccacggcggc cgctgtgaag cctgccaggg tcaggggcag ctgcggatcg agatgcagtt
       61 cctgccggat gtgtacgtcc cctgcgaggt ctgcaacggt gcccgcttta accgggagac
      121 cctgcaggtg cgtttcaaag agaagtccat tgccgaagta ctggaaacga ccatcgacga
      181 gggcgccgga ttcttcgccg ccttcccggc gatgctgcgc aagatcgaga ccctgcgcga
      241 tgtgggcctg ggttacctga agattgggca gccggccacg accctctccg gcggcgaggc
      301 ccagcgagtc aaactctcgc gggagctctc caagcgcggg cccggccaca cagtctacgt
      361 cctggatgaa ccctcggtcg gcctgcatgc agccgacgtg cacaagctga tcgaagtgct
      421 gcagcagctg gtggaccagg acaacacggt cctgatcatc gagcatcacc ccgacatcat
      481 caaagtggcc gactgggtga tcgatctggg cccggaaggc ggcgaccgcg gcggacggct
      541 gctcgccgag ggcacaccgg agcagatcag ccgggtcaaa gcctcctaca caggccaata
      601 ccttaagccg cacctggcga aggcttagac tagtcggacc gctcgcaacc ccgatcaacg
      661 tcagccaaat gtcatcgaat ccactgccgc tgctcctccg gtggtcggtg gtgctttact
      721 gagggatagc tcctcccgta aagtgagtgg gtagccagga ggagggcatg cctacgggat
      781 tcgacgtgat cgtaatcgga gccgggatca tgggctgcag ttcggccttt cagctcgcgg
      841 gtcgtggaat gaaagtgggg gtgtttgaca agcgcgggct gggcgctggg tccaccggaa
      901 aatcctcggc gatcattcgc caacactact ccaacgaagt cacggcccgg atggcgttgc
      961 acagcctgcg ggtcttccag aacttcggtg atgcggtggg cggggaggcc ggcttccgcc
     1021 gagccggttt cctagtcctt actgccgaga aggacgtgga gggactgcgg gccaatgtcg
     1081 ccctccagca gagggtcgga atcaagaccg acatcctctc ggaggcagaa gtcagacgac
     1141 ggtggccgtt tctaaagacc aacgatctgg tcgcggcggc ccacgaaccc gattcgggct
     1201 acgctgaccc aaatctcacc accgggagct acgccaacgc ggcccaagcc aagggagccg
     1261 aattcctcct ggataccgag gtaattggcc tgcagctgcg atcggggaag gttcgagggg
     1321 tgaccacctc gagcgggaca ttcgaagccc cggtcgttgt caattgtgcc ggcccgtggg
     1381 gcgcgagggt tgctcgatgg gccgggatcg aggtgccgat cagctcctgc cgggtgcagg
     1441 tggcattctt tcgccgcccg gatgactatg ccgacgaaca cccggttgtc gccgatttca
     1501 cgactgccag ctattggcgg ccggagacag gaggtttgac cctggtaggt ctgatcgatc
     1561 catccgagct gaaccacgtg gtcgatcctg attcgtacaa cgaaagggtg gagttggatt
     1621 tcgtcgctga cgcgggggat cggctggttc ggcgcttccc tgccatggaa aatagcgcca
     1681 gcgcctccgg ctacgcaggc ttatacgcca tcacgcctga ctggcatccc gtcattgacg
     1741 aactgatcga aggaagcggc ttctacctgt gctcgggatt cagcggccac ggcttcaagc
     1801 ttggtccagc cgtaggctcg atggtcgccg acatggtgag cggggcgatc actcccggca
     1861 tggatcggcg gctgtttagg ctgaatcgat tcgcagagaa cgaaccggta cgaggccggt
     1921 atgagtacag catcgccggc taggatcact cgtagcgcag cgcgtcgatc gggttgagcg
     1981 aggccgcccg ggtggccgga tagatcccga agaagagtcc gaccaggccg gagaagctgg
     2041 tcgccaatag aaccgcctgg agcgagaggg ttggggccag gtcactcgcc gcccggccga
     2101 tcccccaggt gcctaatccg cccaatccga cgcccagtaa tccaccggcg accgccagaa
     2161 ccacgctttc caacaggaac tgcagcagga tgtcccgccg gcgggcgccg acggccttgc
     2221 gcagcccgat ctcgcgggtc cgctcggtga c
//
LOCUS       DATDPQ010000070         6920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2376322, whole genome shotgun sequence.
ACCESSION   DATDPQ010000070 DATDPQ010000000
VERSION     DATDPQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376322"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="rho"
                     /locus_tag="VI520_01595"
     CDS             complement(<1..855)
                     /gene="rho"
                     /locus_tag="VI520_01595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HLF36607.1"
                     /translation="MNIGELEALTLSDLRERSKEMEITGATRMKKEDLILQLMQVEAQ
                     RKGLELRGGVLEIMNEGMGFLRAEHYLPGPNDIYVSQSQIRRFGLRTGDMVIGQIRPP
                     KDTEKYYGLLRVETINGFDPEQIKDRPKFESFTPIFPEERFDLETDRHALANRMLNLI
                     APIGKGQRGLIVSPPKAGKTTVLKGVANAISTKYPKVHLMVALIGERPEEVTDMDRSV
                     DAEVISSTFDDPASSHVRVAEIALERAKRLVECGRDVVILLDSITRLARAYNLMVTPS
                     GRTLSGGLD"
     gene            complement(1028..2023)
                     /gene="glpX"
                     /locus_tag="VI520_01600"
     CDS             complement(1028..2023)
                     /gene="glpX"
                     /locus_tag="VI520_01600"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HLF36608.1"
                     /translation="MGEKPSRNLALELARVTEAASLAAGRHMGRGDKEGGDRAAVDAM
                     RLVLNSIDIDGVIVIGEGEKDQAPMLYNGERLGSGGGPQVDIAVDPIDGTRLLALGRG
                     NSIATVATAERGTMFRPGPFVYMDKIAVGPVAKGQIDLDAPIEDNLKAVARAKAADVG
                     DLTVMILDRPRHEQLIGAVRRAGARIALITDGDVAGAIMTAWPNSGIDVLVGIGGAPE
                     GVLAACALKCMGGEIQARFQPRSPEEAELGRRQGYEPDRRLGIDDLVAGEDVFFAATG
                     ITDGQLLKGVKYSGTGATTHSLVMRSHSGTIREITSIHKWEKLMSFSQIDYDPRT"
     gene            complement(2024..2848)
                     /locus_tag="VI520_01605"
     CDS             complement(2024..2848)
                     /locus_tag="VI520_01605"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLF36609.1"
                     /translation="MLRERLVVTLLLLPILIWTIAKGGWLFAGAAALVLGFAAAEYGL
                     LFRNAGLRPSLQLLVIGVALLVLARVDLPLEWQALTLSGLTLIALTWHLLDYERGALH
                     SATDFAVSLSGIVYLGWIGGYLVLLRSLPEGQWWILIALPAVWIADSAAYLVGSWKGK
                     RLLTPRLSPKKTWEGYLAGVVFGGAAGAGLAGLWSIAAGPDSGLSVVRGLVVGGLIAL
                     LSPLGDLGISMIKREMKVKDAGTLFAAHGGALDRLDSWLWAGVLGYYAVGWLTRGA"
     gene            complement(2849..3565)
                     /locus_tag="VI520_01610"
     CDS             complement(2849..3565)
                     /locus_tag="VI520_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HLF36610.1"
                     /translation="MDEGLPEVVPSHVAIIMDGNGRWARERGLPRLAGHRAGVENLRR
                     VLRAAADFGIDYLTIYAFSTENWERPTGEVRGLINILEEVIDRELRELHGNGVQLRHI
                     GRLEGLRPELQEKVRQAIELTKQNTRLVLNVAWNYGGRDEIIQAIQRMLEDGLDPLSI
                     DETVVNRYLFTADIPDPDLVIRTSGEMRVSNFLIWQSAYSEWWFTPTYWPDFGPEELR
                     HALWAYAQRERRFGTVPALR"
     gene            complement(3576..4133)
                     /gene="frr"
                     /locus_tag="VI520_01615"
     CDS             complement(3576..4133)
                     /gene="frr"
                     /locus_tag="VI520_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLF36611.1"
                     /translation="MIKDALAEAESRMAGAVRALEEDLAAVRTGRASPALAEKVLVDY
                     YGNPTPLIQLATISAPEARLLTIRPFDPGSIKEIERAILSSDLGLTPSNDGKLIRLSI
                     PPLNEERRHELVKVVRARAEEARVAVRNVRRDVHSDLREFEQEKVISKDDLRRGEEQL
                     QKVTDGYIDQISKTSERKESEVLEI"
     gene            complement(4265..4981)
                     /gene="pyrH"
                     /locus_tag="VI520_01620"
     CDS             complement(4265..4981)
                     /gene="pyrH"
                     /locus_tag="VI520_01620"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLF36612.1"
                     /translation="MTEPAYKRVLVKLGGEALAPAEGFGVDPKRAEAVAEKIQDVKAL
                     GVELAIVIGAGNIWRGRVGIERGMERATADYMGMLGTVINSLALMDALERAGTVTRVM
                     SAIEMRALAEPYIQRRARRHLEKGRVVIMGAGTGNPYFSTDTAAALRAMEIGAEIVIK
                     ATKVDGVYSADPAKDPTAKRFDRLTYIQSLNLRLEVMDATAISLCMDNNLPILVLDMW
                     QPDSLVRAVLGEQVGTMVSA"
     gene            complement(5038..5640)
                     /gene="tsf"
                     /locus_tag="VI520_01625"
     CDS             complement(5038..5640)
                     /gene="tsf"
                     /locus_tag="VI520_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLF36613.1"
                     /translation="MSISAELVMQLRQRTGAGVLDCRKALEEAQGEMERAEQILRERG
                     LAKAASRAGRVAQDGIVDLYSHGDGRLGVMVEVNCETDFVARTAEFRHFAHEIALQVA
                     ASAPRWLSSTEVPAEVLEREAELARQQAIGDGKPEKVIDQIVEGRLEKFLDDHCLLRQ
                     AYVRDEEKSINEMVQAMILSTGENVTIRRFQRWEVGEAVG"
     gene            complement(5637..6590)
                     /gene="rpsB"
                     /locus_tag="VI520_01630"
     CDS             complement(5637..6590)
                     /gene="rpsB"
                     /locus_tag="VI520_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLF36614.1"
                     /translation="MPVVNMKELLETGVHFGHRTRKWNPRMKPFIFTERNSIHIIDLQ
                     QTLDSLEEAYALIRQTVASGGSVLFVGTKRQAQESIEQEAIRCGMPFVNSRWLGGTLT
                     NWRTIRSQIDELDDLERRRDEGDFERLIKKEALTLTRRIVKLAARLGGIRNMKAIPHL
                     LFVVDVKHEETAIHEANLLGIPVIGLVDTNCDPRGVDYVIPSNDDAIRAIKLLSATVA
                     NAVLEGVELRKDLPGIHEAGEPSREQVAVPEMADEELLGEATLAKLMSGEFDEEREAQ
                     AARAAAQEQPPAALEQPPAALEQPATEGAPVVEPAETKPQE"
BASE COUNT         1234 a         2275 c         2033 g         1378 t
ORIGIN      
        1 atccaggccg ccggagagag tacgcccgga gggcgtcacc atcaggttgt aggcccgagc
       61 caatcgggtg atggaatcga gcaggatcac cacgtcccgc ccacactcta ccaatcgctt
      121 cgcccgctcg agggcgatct cggcgacccg aacgtgggaa gaggccgggt cgtcaaaggt
      181 ggatgagatg acttcggcgt cgacggagcg atccatgtcg gtgacttcct cgggtcgctc
      241 cccgatcagg gccaccatca gatgcacctt ggggtacttg gtggagatgg cgttggccac
      301 tcccttgagc acggtggtct tccctgcttt gggcggcgag acaatcaggc cgcgctggcc
      361 cttgccgatc ggggcgatca gattcagcat ccggttagcc agggcatgcc ggtcggtctc
      421 gagatcgaat cgctcctcgg ggaagattgg ggtaaaggat tcgaatttgg gacggtcctt
      481 gatctgctcc ggatcgaatc cgttgatggt ctcgacccgc aggaggccgt agtacttctc
      541 ggtgtccttt gggggccgga tctggccgat caccatgtcc ccggtccgca agccaaatcg
      601 tcggatctgc gattgggaaa catagatatc gttcggcccc ggcaagtagt gctcggcacg
      661 caagaagccc atgccttcat tcatgatctc caggaccccg ccccgcagct ccaatccctt
      721 tcgctgcgcc tcaacctgca tcaactgcag gatgaggtcc tccttcttca tgcgcgtggc
      781 gccggtgatt tccatttctt ttgaccgttc gcgcagatca gaaagagtca aggcctccag
      841 ttctccgata ttcatggatt ttctccctag gggaagttat caacccctgt ctggcgggtt
      901 tttcagacta agcgacagaa tctcaacgaa tgagggctag ctgctagtca ggctcaatta
      961 agtagctggg gaagcacgaa ttgtgcggcc aaattataac acccgagccc gtccggcgca
     1021 agcggaccta ggttctaggg tcatagtcga tctggctgaa gctcatcagt ttttcccatt
     1081 tgtgaatcga agtgatctct cggatcgtcc cactgtggga acgcatcacc aggctgtgag
     1141 tcgtggcgcc ggtcccgctg tacttgaccc ccttcaggag ctggccatcg gtgatcccgg
     1201 tcgcggcgaa gaagacgtct tcccctgcta ccaggtcgtc aatccccaag cggcggtcgg
     1261 gctcataccc ttgccggcgg cccagctcgg cctcttccgg cgatcggggt tgaaacctgg
     1321 cttgaatctc gcctcccatg cacttgagcg cacaggcggc gagtacccct tcgggcgcgc
     1381 ccccgatccc gaccaatacg tcgattcctg agttcggcca ggctgtcata atggcgccgg
     1441 cgacgtcccc atcggttatc aacgcaatcc tggccccggc ccgccggacc gcaccgatca
     1501 actgctcgtg ccggggccga tccagaatca tgacggtgag atcaccgacg tccgccgcct
     1561 tggcccgggc aaccgccttc aggttgtcct cgatcggtgc gtccaggtcg atctgacctt
     1621 tcgccaccgg cccgactgcg atcttgtcca tataaacgaa agggccgggc cggaacatgg
     1681 tgccccgctc agccgtggcc acggttgcga tggagtttcc tcggccgagt gcaagcaggc
     1741 gggttccatc aatcggatcg accgcaatgt cgacctgcgg cccgcctccg ctccccagcc
     1801 gttcgccgtt gtagagcatc ggggcctgat ccttctcgcc ctcaccgatc acgatcacgc
     1861 cgtcgatgtc aatcgaattc aacaccagcc gcatcgcgtc cacggctgcc cgatcgccgc
     1921 cttccttgtc accgcgcccc atgtgtcgcc cggcggccaa ggaagcggct tcggtcaccc
     1981 gggcgagctc gagcgccaag ttccgggagg gtttctcgcc cattcaggct ccccgggtca
     2041 gccagccaac agcgtaatat cccagcacgc cggcccacag ccaggaatcc agccggtcca
     2101 gtgctccgcc atgggcggca aagagggtgc cggcgtcttt gaccttcatc tcacgcttga
     2161 tcatgctgat ccccagatcg cccaggggac tcaatagtgc aataaggccg cccaccacca
     2221 agcctcggac tacgctcagg cctgaatcgg gtccggctgc gatgctccac aacccggcca
     2281 ggccggcgcc ggctgctccg ccgaacacaa ccccggccag atagccctcc caggtcttct
     2341 ttgggctaag ccgaggagtc aacaggcgct tccctttcca actgcctacg aggtaggccg
     2401 ccgagtcggc aatccagacc gccggcaagg cgatcaagat ccaccactgg ccttcaggca
     2461 gcgaacgcag caagaccagg taccccccaa tccatcccag gtacacgatt ccggaaagac
     2521 tgacggcaaa gtcggtggcg gaatggaggg cgccccgttc atagtccaac aggtgccagg
     2581 tcagtgcgat cagggtaagc ccggacaggg tgagggcctg ccattccagt ggcaggtcaa
     2641 ctcgggcgag gaccagcagt gcgacgccaa tgaccagcag ctggagcgag ggccgcagcc
     2701 cggcgttgcg gaagagcagt ccgtactcgg ccgccgcgaa ccccaggacc agtgcggccg
     2761 cgccggcaaa taaccaaccg cctttggcga tggtccatat cagaattggg aggagcagaa
     2821 gcgtgacgac cagccgctcg cgcagcatct agcggagggc agggacggtg ccgaagcgtc
     2881 gctcgcgctg tgcgtaagcc caaagcgcat ggcgcagctc ttctgggccg aagtctggcc
     2941 aataggtcgg ggtaaaccac cactcggagt aggcgctctg ccagatcagg aagttgctga
     3001 cccgcatctc gcccgatgtg cggatgacca ggtcgggatc ggggatgtcg gcggtgaaaa
     3061 gatagcggtt gacaactgtc tcatcgatac tgagcgggtc gaggccgtct tcgagcatgc
     3121 gctggatcgc ctggatgatt tcatcccgtc cgccgtagtt ccaggcgaca ttcagcacca
     3181 gccgggtgtt ctgcttggtg agttcgatgg cctgacggac cttttcctgc agttcgggcc
     3241 gcaatccttc cagccggcca atgtgacgga gctgcactcc attcccgtgc agctctctca
     3301 actcgcgatc gatgacctct tcaaggatgt tgatcagccc tcgcacctct ccggtcgggc
     3361 gttcccaatt ctcagtggag aaggcataga tcgtgagata gtcgattccg aagtcggccg
     3421 ccgcccgcag cacccggcgc aagttctcga cccccgcccg gtggccggcc aaccgaggca
     3481 atccccgctc tcgggcccag cgaccgtttc cgtccatgat gatcgcgacg tgggaaggga
     3541 caacttccgg taatccttcg tccatctggc gatcctcaga tctcgaggac ttcgctctcc
     3601 ttgcgctcac tggtcttgct gatttgatcg atgtagccgt cggtgacctt ctgaagctgc
     3661 tcctcgcctc ggcgcaggtc gtccttcgag atcacctttt cctgctcaaa ctcccgcaaa
     3721 tcactgtgga catccctccg gacgttgcgg actgccaccc gggcctcctc ggcccgggcc
     3781 cgcactactt tcaccaattc atgccggcgc tcttcgttga gcggagggat gctcagccga
     3841 atcagtttgc cgtcgttgct tggagtgaga cccaagtcgg aagataggat cgcccgctcg
     3901 atctccttga tactgccggg atcgaatggc cgaatcgtca gcaagcgcgc ttccggggcg
     3961 gagatcgtgg cgagttggat cagcggggtc gggttcccat agtaatcgac caggaccttt
     4021 tccgctaagg cgggagaggc gcgcccagtc cggacggcgg ctagatcctc ttcaagcgct
     4081 cggactgcgc ccgccatccg agattcggcc tccgccaggg catccttgat catccgccgg
     4141 ctccaatctg ttcgccagtg tctgtgattt caagatagca tagcccaaaa cgactgtttt
     4201 gcgaagcaag cctagattgg ggggagggcc gctcgattgc cggccgcagc aggatgggcc
     4261 gaaactatgc gctgaccatc gttccgacct gctctcccag cacggccctg accaggctgt
     4321 ctggctgcca catgtccagg accaggatcg ggagattgtt gtccatacat agggagatgg
     4381 ccgtggcgtc catgacctcg aggcgcagat tcagggattg gatataggtc aggcggtcaa
     4441 agcgcttggc agtggggtcc ttggccggat cggcgctgta taccccgtcc accttggtgg
     4501 ccttgatcac gatttcggcg ccgatttcca tggcccgcag cgccgctgcg gtatcggtgg
     4561 agaagtacgg gttgccggtt ccggccccca tgatcaccac gcgacccttt tcgaggtggc
     4621 gccgggcgcg gcgctgaatg tagggctcgg ccagcgctcg catctcaatt gcgctcatca
     4681 cccgggtgac ggtgcccgct cgttcgagcg cgtccatgag ggccagcgaa ttgatcaccg
     4741 tgcccagcat ccccatgtaa tccgcggtag cacgttccat cccgcgttcg atcccgaccc
     4801 ggccgcgcca gatattgccg gctccgatca cgatggccag ttccactccg agggccttca
     4861 cgtcctggat cttctcggcc accgcttccg cccgcttcgg gtctacccca aagccttcgg
     4921 caggcgccag cgcctcccct ccgagcttca cgagcactcg cttgtaggct ggttcagtca
     4981 ttccgccggt caggtctgat tcatgaaaac gggccaaccc ctggttggcc cgtgtgctca
     5041 gcccactgcc tctccaacct cccagcgctg aaaccgccgg atcgtcacat tctccccggt
     5101 agagaggatc atcgcttgaa ccatctcgtt aatggacttc tcttcgtccc gcacgtaggc
     5161 ttggcgcagc aggcaatggt cgtccaggaa cttctccaac cgaccttcca caatctggtc
     5221 gatcaccttc tccggtttcc cgtcgccgat ggcctgttgg cgggcgagct cggcctcccg
     5281 ctccagcacc tctgccggca cctcggttga actcagccag cgcggggcgc tggctgcaac
     5341 ctgcagggcg atctcatgcg cgaagtggcg aaactcggca gtgcgggcca cgaagtcggt
     5401 ctcacagttg acttccacca tcaccccgag ccgaccgtca ccatggctgt acaggtcgac
     5461 gatcccgtcc tgggcgaccc ggccggctcg actggcggcc ttggccaatc cccgctcgcg
     5521 caagatctgc tctgctcgct ccatttcgcc ctgggcctct tccagagctt tgcggcagtc
     5581 caagaccccg gcgccggtac gttggcgtag ctgcatgacg agttcggccg aaatgctcat
     5641 tcctgcggtt tggtctccgc cggctcaacg accggggcgc cttcggtggc cggctgttcc
     5701 agtgcggcgg gcggttgctc caatgcggcg ggcggctgtt cctgcgcggc ggccctcgcc
     5761 gcctgtgctt cgcgctcctc atcgaactct ccgctcatga gcttggccaa ggtggcctcg
     5821 ccgagcagtt cttcgtccgc catttctgga actgcgacct gctcgcggga gggttcgcct
     5881 gcctcgtgga tccccggaag atccttccgc agctcgactc cctccaagac cgcgttggca
     5941 acggtcgccg acagcaactt gatggcccgg atcgcgtcat cattggaggg gatgacgtag
     6001 tccactcccc gcggatcgca attggtgtct accaggccga tcaccgggat gcccagaaga
     6061 ttggcttcat ggatagccgt ttcctcgtgc ttcacatcca ccacgaaaag caaatgcggg
     6121 atcgccttca tgttgcgaat cccgcctagc cgcgcggcaa gcttgacaat ccgacgggtc
     6181 agagtgaggg cctccttctt gatcagccgc tcaaaatcgc cttcgtctcg cctgcgttcc
     6241 aggtcatcca actcgtcgat ctgactgcgg atcgtgcgcc agttggtgag ggtcccaccc
     6301 agccagcggg aattgacgaa tggcatcccg cagcggatgg cctcctgttc gattgactcc
     6361 tgagcctggc gcttagttcc cacaaagagc accgaacctc cgctagccac cgtctgccgg
     6421 atcagtgcgt aagcttcctc cagcgagtcg agagtctgtt gcaggtcgat gatgtggatg
     6481 ctgttgcgtt cggtgaagat gaagggcttc atccgcgggt tccacttgcg agtgcggtgc
     6541 ccaaagtgca ccccggtctc taatagctcc ttcatgttca cgactggcat ctgacctcca
     6601 aaagctcggc agcagaaccg cgaatctcgg ctcgagccat aatcgggccc gagtttgtgg
     6661 ttcacccgcc gggcagaaac tctcccgcca ggctgctagc agccgtgagc gggagcgccc
     6721 ggcagtatat catactcgac ggctagaccg cgggaatgtt gaatgcgaca gccacggcca
     6781 ccgcgtgttg ctcggtgtgg ctcaagctga ttgcccaatc ggtgaggccc atggagatgg
     6841 ccagctcatg agcctggccg tgcaaccgca gctgaggttc ccggcgcgga ccgttgacca
     6901 cttcgatttc cttccaggca
//
LOCUS       DATDPQ010000071         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_285931, whole genome shotgun sequence.
ACCESSION   DATDPQ010000071 DATDPQ010000000
VERSION     DATDPQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285931"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..2281
                     /locus_tag="VI520_01635"
     CDS             107..2281
                     /locus_tag="VI520_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF36615.1"
                     /translation="MPTLRRWFTMGALLALAGFQSGPSSVHIHETLEPQQTTSTSGNL
                     GLSLQTTFSLLDGEGQVLRADIESGTFELEGDAYPATVEALETPWSLVLLIDASKTLG
                     TGTASATWKSVRSTIGAALAGFPENSNIAILKFDDTAPTVLEFTQAKDRLAAAVQALS
                     PKPSGGSCLNEGIYQAVNKLGGVPGRRAVILFTASTDNCGTRTASEVISLASQNRVQL
                     YPVGLLGYAVTEAELEALAGPTGGIAEFKEQSTLTFGLANVNALLSNQWTAKATVYPS
                     AGQQSATLKVGLKDDISLVSAPITFVSSQDFVPPSEIHLRGKVLSEGEGILFNLDIIQ
                     AEKIRQLNVSIISKDTGQAVLSQSLISFSDVNTIATAGLIPGGEYTLIVSAIDASGRL
                     LSETSGEFTFEPPPARLNVTEVQPPTAEQPDFLVTVTTQNLVGVVKFKGWLADAQSNV
                     PLDDTERTVPLGEPLLLPADGLASGTYAVVVQALDVADTVLAETTPVKVSYTRPSPLE
                     SLRTFVSSSPAAVAGLTGLFCLTVAGVGAVVWLLIPKRGTRPVTVDLVMPQKARRPAP
                     GAERTPSNLAAIQPAPAPERPPAHRDRPTPQQGLERPPAGQPPVGLPKPSAAHAMPPA
                     RLTLVHPAAPQFSAEIRRSPFMIGRRPGGDAVIPLDSSSGVSGQHFRITFVDGQFFIQ
                     DEKSTFGTTLDGSPLVQGRPMPLRDGSVIGLGPQVRIRFNLT"
     gene            2321..>3153
                     /locus_tag="VI520_01640"
     CDS             2321..>3153
                     /locus_tag="VI520_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HLF36616.1"
                     /translation="MTAPRIRGDYDLLAQIAQAFGQHASQTKASLLRIQREVNTLQSG
                     DWIGTGATAFYREMNDQVLPSLKRLGAALETADRTLRQVSQIIKQAEEEAARLLRLDG
                     SGALGAALGGGLGGALGGTAGSVGELLGGQTPASFLARDPAGLFSGDYMRGLIGLQIQ
                     GAGSELGDAMNGLMGATGADDAEGYLVIIAELRGRPLDDIRSEFDRFQEVRGQRDAAV
                     AAGKDAVPGLSGVNSGFNGSNTQMRYGQMVGDAFGIDPVFGAMLNPTGGLVGPGNFTL
                     HP"
BASE COUNT          563 a         1029 c          992 g          569 t
ORIGIN      
        1 ggcacccatc cgggtggcgg gataaccggc gccccgggta ggctggccgc actgcaggtc
       61 ctgaaggact ggggtcgatt gcgcatataa tattcgccgt ccgtccatgc ccactctccg
      121 ccgctggttc acaatgggtg cattgctcgc gctggccgga ttccaatcgg gtccttcaag
      181 cgtccatatc catgaaaccc tcgagcccca gcagaccacc tcgacctccg gcaacctggg
      241 gctttcactc caaaccacct tcagcctgct ggacggcgag gggcaggtgc tgcgagccga
      301 catcgaaagt gggaccttcg aactcgaagg cgacgcctac cctgcgaccg tcgaggcgct
      361 ggaaaccccc tggtcactgg tgctcctgat tgatgccagc aagacgttgg gcaccggcac
      421 cgccagtgcg acttggaagt cggtcagaag cacgatcgga gctgcgttgg ccggctttcc
      481 cgagaattcg aacatcgcga tcctgaagtt tgacgacacc gccccaaccg tgttggaatt
      541 cacccaagcc aaggaccggc tggcggcggc ggttcaggcg ctgagcccca aaccgagcgg
      601 gggatcctgt ctcaatgagg gcatatatca ggcggtcaac aaattgggcg gagtacccgg
      661 acggcgggcg gtcatcttgt ttaccgccag caccgacaac tgcggcactc gcacggcatc
      721 agaggtcatc agcctggcaa gtcagaatcg agtacagctc tatccggtcg gactgcttgg
      781 ctacgcggtc accgaggcgg agctggaggc ccttgccggt ccgaccgggg gcattgccga
      841 attcaaggag cagagcacgc tgaccttcgg gcttgccaat gtaaacgcgc tcttgagcaa
      901 ccagtggacc gccaaggcca ccgtctaccc ttcggccggg caacagtctg cgaccttgaa
      961 ggtgggcttg aaagacgaca tcagcctggt ttcggccccg atcaccttcg tttccagcca
     1021 ggactttgtt ccgccctccg aaatccatct taggggtaag gtcctttccg agggtgaggg
     1081 gatccttttc aatctggaca tcatccaagc cgagaagatc cgccagctga acgtcagcat
     1141 catcagcaaa gacaccggtc aggcggtgct atcccaatcc ctgattagct tctccgacgt
     1201 caatacgatt gccacggccg gcctgattcc gggcggggaa tacactctga tcgtaagtgc
     1261 gatcgatgcc tccggccgac tgttgtcgga gaccagcggc gaattcacgt tcgaacctcc
     1321 cccggcgcga ttgaacgtaa ccgaggttca accccccacc gccgagcaac ccgacttcct
     1381 ggtgaccgtg accacccaga acttggtggg ggttgtaaag ttcaagggtt ggctggccga
     1441 cgctcagagc aatgttccgc tcgatgacac cgagcggacc gtccccctgg gcgagccgct
     1501 gctcctgccc gccgacgggc ttgcctccgg cacttacgcg gtcgtcgtgc aggcgctgga
     1561 tgtggcggat accgttctgg ctgagaccac tccggtcaag gtcagctaca cccgccccag
     1621 tccgcttgag agcctgcgca ccttcgtgag cagctcaccc gcggcggtcg ccgggctgac
     1681 cgggcttttc tgcctcaccg tagcaggcgt cggggcggtg gtctggctgc tcatcccaaa
     1741 gcgcggtacc cgacccgtta ccgtagatct cgtcatgccc cagaaggctc gccgccccgc
     1801 gccaggagcc gagcgcactc cgagcaactt ggcggccatc cagcctgctc ctgctcccga
     1861 gcggccgccg gcccatcggg accggccgac cccgcagcaa ggtctcgagc ggccgcccgc
     1921 cgggcaaccg ccggttgggc tgccaaaacc cagcgccgcc catgcgatgc cgcccgctcg
     1981 cctgaccctg gtccacccgg cggcccctca gttttcagcc gagattcgcc gctccccatt
     2041 catgattggg cgccgccccg ggggcgacgc ggtcatcccg ctcgacagct cgagcggtgt
     2101 gtcggggcag cactttcgga tcacgttcgt cgacggacag ttcttcattc aagacgagaa
     2161 gagcaccttc ggcaccacct tggacggcag cccgctagtg caagggagac cgatgccttt
     2221 gcgcgacgga tctgtgatcg ggttggggcc tcaagttcgg atccgcttca acttgacatg
     2281 agtgtgtcgg tagccaacca gtgagacgga gggcgcaatc atgacagctc ctcgaattcg
     2341 cggtgattac gatttgctgg ctcagatcgc acaggccttc ggccaacacg cgtcgcagac
     2401 caaggcctct ttgctgcgga tccagcgcga ggtgaatact ctccagagcg gcgactggat
     2461 tgggaccggg gccacggcct tctatcggga gatgaatgac caggtgctcc cgagcctcaa
     2521 gcggcttggc gctgccctgg aaacggccga ccggactctg cgccaggtca gccagatcat
     2581 taagcaggcc gaggaagagg ctgcccgact gctccgcctg gacgggtcgg gagcgctggg
     2641 tgcagcgctg gggggtggcc tgggcggcgc cctcggagga accgcgggtt cagtcggtga
     2701 gctcctgggt gggcagacgc ctgcttcgtt cttggcccgc gatccggccg gtctgttcag
     2761 cggcgattac atgcgcggac taatcggcct gcagattcag ggcgccggca gcgagctggg
     2821 cgatgccatg aatggcttga tgggggcgac cggcgccgac gacgccgagg gatacctggt
     2881 gatcatcgct gagctgcgcg gccggccgct ggatgacatc cgctcagagt ttgatcgttt
     2941 tcaggaggtc cgcgggcagc gcgatgcggc ggtcgcggcc ggcaaggatg cggttcccgg
     3001 cctgtcgggc gtcaactccg gcttcaatgg ttcaaacact caaatgcgct acggtcagat
     3061 ggtgggcgac gccttcggga tcgacccggt tttcggcgct atgttgaacc ccaccggtgg
     3121 cctggtcggg ccaggcaatt tcaccctcca ccc
//
LOCUS       DATDPQ010000072         3944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1596479, whole genome shotgun sequence.
ACCESSION   DATDPQ010000072 DATDPQ010000000
VERSION     DATDPQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596479"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2374
                     /locus_tag="VI520_01645"
     CDS             <1..2374
                     /locus_tag="VI520_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLF36617.1"
                     /translation="PVGMCRMCLVEIGRPARDRATGQPVFSEQGAPVIQFGANLETAC
                     TTQVGEGWQVRVFSGKAIEGRKAIVEYLLTSHPLDCPICDKGGECPLQNLTMEHGAGL
                     SRFLFDEKQHLAKHLALGELIFLDRERCIQCARCTRFQDEVADDPVIGLSQRGRRLEI
                     VTYSDPGFDSYFSGNSTDICPVGALTTADFRFGARPWELNAAASICPHCPVGCNLTLN
                     TRRAAGGDGRETIKRVMPRQNEQVNEIWICDKGRFAHHYAHSADRLSRPLVRENGTLV
                     EASWEAALNRAADLLGRLGAGLLGLAGGRLSNEDLFNFRALIEGLGGTPVMDDWMAGG
                     DLVRQFGVYPGTNLGTLGQGDAVLVIATDLHEEAPIWWLRIKQAAERGAELIVANARQ
                     TRLDSYSTQQLRYAPGDAVRAGLGLLGALMGDPKLQSYVEDRQTTAAAKALSAARNLI
                     VVYGNEGLDYPGSEALARACARLLSAGNHLGRSNNGLIAVWPRANTQGAWDMGLRPLP
                     AGLAGRLEQSTGAYVVAADPVGDDPTVGQLLTGLRGLVVQDLFLSPTAELADVVLPVQ
                     SFVEREGTFTSGERRVQRLYPAMPGPVESLADWKILAELGHRLGLELEGRSAGQVFAR
                     IAAQIPDYAGLNYTDLAVSARQWPEVGGEDLYYGGTAYDNRQGLGVQLAPRPGGEPPE
                     WLAAAERRPSGDLLLIPISRLYDRGATVLASELLSLRLAELKLAIHPEDAERLGLIGA
                     SRVELSWDGRASTVEFAIEQDVPRGAGLVVRSTGLPLNRPTEVGVRPASS"
     gene            2371..3369
                     /gene="nuoH"
                     /locus_tag="VI520_01650"
     CDS             2371..3369
                     /gene="nuoH"
                     /locus_tag="VI520_01650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HLF36618.1"
                     /translation="MIDLLEWTLESLGIITIVLGGFAYLTLFERRVMARFQTRIGPNR
                     TGPLGLLQPIADGIKLLFKEELIPDQADKLLFVLAPVITVVPVLLLMAVIPFGPDLTI
                     LGRQVSQGLAPEVNVGALYILAIASIAVYGIVIAGWASNSKYALLGGIRSSAQMISYE
                     LALGLAFIGPILLADSLNMAEIVRAQRGLWFVFLQPIGFLIFLVASIAELNRAPFDMP
                     EAEQELTAGYHTEYSGMKFALFFMAEYGKMIAVSFIAVTLFLGGYWGPFVDRLPILGP
                     FYLIAKVVGLLFLMIWLRATLPRLRYDRLMAFGWKVLLPLGLLNVLVTGGLIVALG"
     gene            3375..3878
                     /gene="nuoI"
                     /locus_tag="VI520_01655"
     CDS             3375..3878
                     /gene="nuoI"
                     /locus_tag="VI520_01655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HLF36619.1"
                     /translation="MSPLQILRGMWTTFRMLFERPVTYQYPEVKRPVRERFKGRHELK
                     RYDDGLEKCIGCSLCAAACPADAIFVESAENTDEVRFSPGERYASTYEINMLRCIFCG
                     YCEDACPTEAIVLEHNYELSFTDRRGAIYTREMLLVPVPPGGKPTPQQVPPGAFDRSI
                     PDMQDPD"
BASE COUNT          670 a         1174 c         1320 g          780 t
ORIGIN      
        1 gccggtcggg atgtgccgca tgtgcctggt ggagatcggc cgcccggccc gcgaccgagc
       61 caccggacaa ccggtcttca gcgagcaggg agcaccggtc atccagttcg gggccaatct
      121 cgaaaccgcc tgcacgaccc aggtgggcga aggctggcag gtccgggttt tcagcggcaa
      181 ggcgatcgaa ggccgcaagg cgatcgtcga gtatctgctg acctcccatc ccctcgattg
      241 tccgatctgc gacaagggcg gggagtgccc gctgcagaat ctcaccatgg aacacggagc
      301 cggcctcagt cgcttcctgt tcgacgagaa gcagcatctg gcgaaacatt tggcgctcgg
      361 tgagctgatc ttcctggatc gagaacgctg catccagtgt gcccgctgca cccgcttcca
      421 ggacgaggtg gcggatgatc cggtcatcgg actctcgcag cgcggccggc ggctcgagat
      481 tgtgacttac tcggatcccg gcttcgattc ctacttctcg ggtaactcga ccgatatctg
      541 tccggtcggc gctcttacca cggccgactt tcggttcggg gcccggccct gggagctcaa
      601 cgccgcagcc tcgatctgtc cccattgtcc ggtgggatgt aatttgaccc tcaatactcg
      661 ccgggcggcc ggcggggacg ggcgcgagac catcaagcgg gtgatgccgc gccagaatga
      721 gcaggtcaac gagatctgga tctgcgataa gggccgtttc gcccatcact acgctcacag
      781 cgccgaccgg ctgtccagac cgttggtccg tgagaacgga accctggtcg aggcgtcatg
      841 ggaagccgcg ctcaatcgcg ccgccgatct gctgggccgg ctcggagcgg gattgctggg
      901 cctggcgggg gggcggcttt cgaacgaaga ccttttcaat ttccgggcgt tgattgaagg
      961 gctggggggc acaccggtaa tggacgactg gatggccggc ggagacctgg tgcgccagtt
     1021 cggggtgtat ccgggaacca atcttggtac gctggggcag ggggatgcgg tcctggtgat
     1081 cgcgaccgat ctccatgaag aggcacccat ctggtggttg aggatcaaac aggctgccga
     1141 acgcggggcg gagctgatcg tcgccaacgc ccgccagacc cggctggatt cgtactcgac
     1201 ccaacaactg cgctacgccc cgggcgatgc cgtgcgggcc gggctcggac tgttgggcgc
     1261 gctgatgggc gaccccaaac tgcagagcta cgtcgaggat cggcagacca ccgccgcagc
     1321 gaaggcgctt agcgctgcac gtaacctgat cgttgtctac ggtaatgaag ggttggacta
     1381 ccccggctcc gaggccctgg cccgggcctg cgcccgcttg ctcagcgccg gcaaccacct
     1441 tggccgcagc aacaacggtt tgatcgcagt ttggccgcgg gccaacaccc agggagcctg
     1501 ggatatgggc ctgcggccgt tgccagcggg cctggcgggc agacttgaac aaagcaccgg
     1561 tgcctacgtt gtggcggcgg acccggtcgg ggacgatcca acggttgggc agctcctaac
     1621 cgggctgcgg gggttggtgg tacaggatct gtttttgagt ccaactgccg agctggccga
     1681 cgttgtgctg cccgtccagt ccttcgtgga gcgggagggg accttcactt ccggcgaacg
     1741 gcgggttcag cggctgtatc ctgcgatgcc gggaccggtt gagagcctgg cggactggaa
     1801 gattctcgcc gaacttgggc accgattggg actggagttg gagggtagaa gtgcagggca
     1861 ggtctttgcc cgaatcgccg cccagattcc ggactatgcc gggctgaact ataccgacct
     1921 ggccgtcagc gcccggcaat ggccggaggt cggcggagag gatctctatt acggcggtac
     1981 tgcctacgat aaccggcagg ggcttggggt gcagctagcc cctcggccag gcggggaacc
     2041 tccggaatgg ctagcggccg cggagcgcag gccgagtggg gacttgctct tgatcccgat
     2101 cagcaggttg tatgaccgcg gggctacagt gctggcttcg gaactgctga gcctgcggct
     2161 ggccgagctg aaacttgcta tccatccgga agatgccgaa cgacttgggc tgattggtgc
     2221 ttcccgagtt gagctttcct gggacggccg ggcgagcacc gtcgaatttg cgattgagca
     2281 ggacgtgccg cggggggcag gcctggtggt aaggagcacc gggctgccgc tcaaccggcc
     2341 gaccgaagtt ggggtgaggc cggcaagctc gtgattgacc tgctggagtg gaccctcgag
     2401 tcgctgggga ttatcaccat cgtgctgggc ggtttcgcct acctgaccct gtttgagcgc
     2461 cgggtaatgg cacgcttcca gacccggatt ggcccgaacc ggaccggacc gctggggttg
     2521 ctccagccga tcgccgacgg gatcaagctt ctcttcaagg aagagttgat ccccgaccag
     2581 gcggacaagc tgttgttcgt gttggccccg gtgatcacgg tcgtgccagt cctattgttg
     2641 atggccgtga ttccgtttgg accggacctg accatccttg ggcggcaggt gagccagggt
     2701 ctggctccgg aagtcaatgt tggagcgctg tatattctgg cgatcgcttc gatcgcggtc
     2761 tacggaatcg tgatcgccgg ctgggcctcc aacagcaagt atgcactgct cggcggcatt
     2821 cgatcttccg cccagatgat cagctacgaa ctggcgctgg ggctggcatt catcgggcct
     2881 attctgctgg ccgactcctt gaatatggcc gagatcgtac gggcccaacg cgggctctgg
     2941 ttcgtcttcc tgcagccgat cgggttcctg atttttttgg ttgcgtcgat cgccgagctc
     3001 aatcgggctc ccttcgatat gccggaggcg gagcaggaat tgaccgctgg ctaccatacc
     3061 gaatactccg ggatgaaatt cgcgctcttt ttcatggccg agtacggcaa aatgatcgca
     3121 gttagtttca tcgccgtgac cctcttcctt ggcgggtatt ggggaccatt cgtcgaccgg
     3181 ttgccaatcc tgggcccgtt ctacctcatc gcaaaagttg tggggttgct cttcctgatg
     3241 atctggctgc gggcgaccct gccccggctc cgctacgatc ggctgatggc atttggctgg
     3301 aaggtcttgc tgccgctcgg gctgctgaac gtactggtga ccggcggctt gattgtggcg
     3361 ctaggttgat cgggatgagt ccgctccaaa ttctgcgcgg aatgtggacg acgtttcgca
     3421 tgctcttcga acggccggtg acctaccaat atccggaggt caaacgcccg gtgcgcgagc
     3481 gcttcaaagg gcggcacgag ctcaagcgct acgacgacgg cttagaaaag tgcatcggct
     3541 gttcgctgtg cgcggcggcc tgcccggccg acgctatctt cgtcgagtcg gccgagaaca
     3601 ccgacgaagt gcggttttcg ccaggcgagc gctatgccag cacgtacgaa atcaacatgc
     3661 tgcgctgcat cttctgcggc tactgtgaag acgcctgtcc caccgaagcg atcgtccttg
     3721 aacacaacta tgaattgtcg ttcaccgatc gccggggggc aatctacacc cgggagatgc
     3781 tcttggtgcc ggtgccaccc gggggcaagc cgactcccca gcaggttccc cccggcgcat
     3841 tcgaccggtc catccccgac atgcaggatc cggattgaat agactcgagg gccgcggttg
     3901 actgccgaac tggcgctctt tttcgggctg gcaatcgtgg cggt
//
LOCUS       DATDPQ010000073         4120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_545012, whole genome shotgun sequence.
ACCESSION   DATDPQ010000073 DATDPQ010000000
VERSION     DATDPQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545012"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1179)
                     /locus_tag="VI520_01660"
     CDS             complement(61..1179)
                     /locus_tag="VI520_01660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLF36620.1"
                     /translation="MLTPKHIARADREQTLRNRILAGAGLTLLLVIGLLTYGWIQVNV
                     IQPPQPVAIVNGQEVSTGTFRDRVKLVQYNLQSQYSSLTSILAVVGDDAQARSTYQSQ
                     LNNVSQQLGNPLFVGSSVMDGLIQEVLVEQEAKRRGLLVTEQDLDQAVEENFGFSESG
                     PTPSPPSTTPGAAVATPYTRDLFERNYQAFLTNLGSFGVNESAVRSELQAQLYRERLG
                     QTFEAEVPRVQEQVWARHILVAEETLAADLLEQVRSGADWEVLAAAHTTDEAGKANGG
                     DLGWFSRGRMVEPFEAAAFAGETGDIVGPVQSDFGWHIIEILGHEDRDLSDSDFRSAV
                     ALAFDEWLSTSRDAAEITIRDYWIERVPSAPILPVSQG"
     gene            complement(1220..1489)
                     /gene="rpsR"
                     /locus_tag="VI520_01665"
     CDS             complement(1220..1489)
                     /gene="rpsR"
                     /locus_tag="VI520_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HLF36621.1"
                     /translation="MSEEDHEQIESGPARRFRRKPRACQFCADQAESIDHKQTELLKR
                     FTYDSGKIRPRRDSGACARHQRMLGRAIKRARHLALLPFAAERFR"
     gene            complement(1486..1872)
                     /gene="rpsF"
                     /locus_tag="VI520_01670"
     CDS             complement(1486..1872)
                     /gene="rpsF"
                     /locus_tag="VI520_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLF36622.1"
                     /translation="MRKYEVAFIADPDLDEEALTALEERVKSWVEAGQGKTLQVDRWG
                     KRRFAYPIGRRTEGYYVFIQTEMPPAAGLAIERDLRLSEQVLRFLITLQEGVQPPARD
                     PRPSISPGLTPISSEPPEGPAREANT"
     gene            2094..3521
                     /locus_tag="VI520_01675"
     CDS             2094..3521
                     /locus_tag="VI520_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HLF36623.1"
                     /translation="MTTDPIRFGTDGWRGRIAEDFTFAAVRRATHGYAQYLLKAEGSS
                     RPVIVGYDKRFASEHFAECAAEVLAANGLSVLLTREATPTPVISFAVVANSAAGAVNI
                     TASHNPPGDNGFKVRDHNGGAIAPQGLAEIEARIPDTADSVPRLAFAGGIRSGSIAYF
                     DPIPPYVSQIQGLVDLERLRAAGLTVMVDAMWGNGAGWFGRFLTGGTTQVFQIHGGRN
                     PIFPEMSRPEPIPPNVDVGLAAAKSQAADVLLVTDGDADRLGVGDEHGEFVDQLRVFA
                     LLAYYLLEVRGARGPIVKTLSTTYLLHKLGSAYQVPVYETGVGFKYVAPKMLEVDALI
                     GGEESGGYAFRHHVPERDGILAGLYLLDMMVQLGRKPSELIKLLFEKVGPSYYKRIDT
                     PLEPAERKAREQVIQSADPVRVGGLRVTSINRTDGHKFELEDGGWLLVRFSGTEPIVR
                     VYCETTQEKRVEPILADGLRLLGVS"
     gene            3518..>4120
                     /locus_tag="VI520_01680"
     CDS             3518..>4120
                     /locus_tag="VI520_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLF36624.1"
                     /translation="MNHRLTDTHCHLSLPEFSGDLDETLERAQASGIGTLIVPGIDLP
                     TSRMAVSLAESHPGLFAAVGIHPHEAESWTPAAGVELRKLARSPTVKAIGEIGLDYYR
                     DLAARPAQRAALQAQLEIAGDLRLPVIVHNRAAIEELLAVLIEWSGGLDPRLRSRAGV
                     LHAFSGELEHAQTAIGSGFFIGVAGPLTYPNAQPLRELVQR"
BASE COUNT          756 a         1286 c         1290 g          788 t
ORIGIN      
        1 aggggtggcg actcctccgg gcaaccggcc ggggaatgtt gcaaccgtgg gtagaccggg
       61 tcagccctgg ctaacgggga ggatcggagc cgatggcacc cgttcgatcc agtagtcgcg
      121 aatcgtgatc tcggccgcgt ctcgcgacgt gctcaaccac tcatcaaagg cgagggcgac
      181 cgcagatcga aaatcggaat cgctcaggtc tcgatcttcg tgccccagga tctcaataat
      241 atgccagccg aaatcgctct ggactggtcc gacgatgtct ccagtctcgc cggcaaatgc
      301 ggccgcttcg aaaggttcga ccatgcggcc ccgggaaaac catccgaggt cgccgccatt
      361 tgccttgccg gcctcgtcgg ttgtgtgcgc cgctgccagc acttcccagt cggctcccga
      421 gcggacctgt tccagcagat ctgctgccag cgtctcttcg gccacgagaa tgtgccgcgc
      481 ccagacttgc tcttgcaccc gggggacctc ggcttcgaat gtctgtccca gccgctcgcg
      541 atagagctga gcctgcagct cggaacgaac tgcgctttca ttgaccccga atgacccgag
      601 gttggttaga aaggcctggt agttccgttc gaacaggtct cgggtatagg gagtggcgac
      661 cgcggcaccg ggggttgtgc tgggcgggct cggggtggga ccggattcgc tgaacccgaa
      721 gttctcctcc acagcctgat cgagatcctg ctcggtcacc aggagcccgc ggcgcttggc
      781 ttcctgctcg accaagacct cctggatcaa cccatccatg accgaactcc caacaaacag
      841 tggatttccc aactgctggg acacgttgtt caactgggat tggtaagtcg aacgagcctg
      901 cgcgtcgtcc cccacaacag ccaggatgct ggtaaggctc gaatactgag actgcaagtt
      961 gtactgcacc agcttcaccc gatcgcggaa agtaccggtc gagacttcct gcccgttgac
     1021 aatcgccacg ggctggggcg gctggatcac gtttacctgg atccagccat aggtcagcaa
     1081 cccaatcacc agcagcagag tgaggcctgc gccggcgaga atgcggttgc gaagggtttg
     1141 ttcccgatct gctctggcga tgtgcttggg agttagcaag gaccccgcag ttaggtcggg
     1201 atgcccggtt gctgcccggt tagcggaacc gctccgctgc gaagggaagc agcgctaggt
     1261 ggcgggctcg tttgatcgcc cgaccgagca tccgttgatg gcgggcgcag gccccgctgt
     1321 ctcggcgcgg ccggatcttc ccagaatcgt aggtaaagcg cttcaataac tcggtctgct
     1381 tgtggtcgat cgattcggcc tggtcggcgc aaaactgaca ggcgcgtggc ttgcggcgga
     1441 agcgcctggc cggcccagac tcgatctgct catgatcctc ttcgctcaag tatttgcctc
     1501 ccttgcgggc ccctcggggg gctcgctgct gattggggtt aggccaggac tgatactcgg
     1561 cctcgggtca cgcgccggcg gctggacccc ctcttgtagg gtgatcagaa atcggaggac
     1621 ttgttcgctc aaccggaggt cccgctcgat cgccaggccc gcggccgggg gcatttcggt
     1681 ttgaatgaat acataatagc cctcggtccg cctaccgatc gggtaagcaa acctgcgctt
     1741 cccccatcga tcgacctgca gggtctttcc ctgcccggcc tccacccagg atttcactcg
     1801 ttcctcgagc gcggtcaatg cctcctcatc aaggtcgggg tccgcgataa atgcaacttc
     1861 gtactttcgc actgcagaag ctcctttcca cggggctgcc ccctcctcga cacgaggttg
     1921 tgccggaggg agcggaaagc taaaatggat tgagtttcga agtttatcac agggccgccg
     1981 ctgcggcaac gccacgcgtt tgtgccatat ttcacggtat aatccccctc tgccccggcg
     2041 aaccacccaa cataacaatc agcccagccc gctttcggca ggtaccatac gacatgacga
     2101 cggatcccat ccggtttgga accgacggtt ggcgaggcag gatcgcggag gactttacct
     2161 ttgccgccgt acggcgagca actcacggct atgcgcaata tcttctgaaa gccgagggtt
     2221 cgagccggcc ggtcattgtc gggtacgaca agcgcttcgc ctcggagcat ttcgcggagt
     2281 gtgccgcaga ggtcctggcg gccaacggtc tctcggtcct gctcacccgg gaggccacgc
     2341 cgaccccggt catctccttc gcggtggtag cgaactccgc agccggggca gtcaatatca
     2401 ccgcttccca caacccaccc ggggacaacg gattcaaggt ccgcgatcac aacgggggcg
     2461 caatcgcccc ccaggggctc gccgagatcg aggcccgaat cccggataca gcagattcag
     2521 tgcccaggct cgccttcgct ggaggaatcc gatctggatc gatcgcctac ttcgatccga
     2581 tccctcccta tgtaagccag atccagggtc tggttgacct cgagcggctg cgggcagcag
     2641 gcctgactgt gatggtggat gcgatgtggg ggaacggcgc cggctggttc gggcgcttcc
     2701 tgaccggcgg aacgacccaa gtattccaga tccacggcgg gcgcaacccg atcttcccgg
     2761 aaatgagccg cccggagcca atcccaccta acgtggatgt cggtctggcg gctgccaagt
     2821 cgcaggcggc cgacgtcctg ctggtcaccg acggagacgc ggaccgcctg ggagtcgggg
     2881 acgagcacgg tgaattcgtg gatcagctgc gggtcttcgc gttgttggcc tactatttgt
     2941 tggaggtgcg cggagcacgc ggtccaatcg taaagaccct gtccacgacc tacctgctgc
     3001 acaagctggg atcggcctat caggttccgg tgtacgagac cggagttggc ttcaagtatg
     3061 tcgccccgaa gatgctggag gtcgacgcat tgatcggagg cgaagaatcc ggcggctacg
     3121 cattccgtca tcacgtgccg gagcgggatg gaatcctcgc cgggttgtac ctgctcgaca
     3181 tgatggtcca gctcgggcgg aagccctccg agctgatcaa gctgctcttc gaaaaggtgg
     3241 gccccagcta ctataagcgg atcgataccc cgcttgagcc ggccgaacgc aaggcacgcg
     3301 agcaagtgat ccagtcggca gacccggttc gggttggagg actccgagtg acctctatca
     3361 accggaccga tggacacaag tttgagctcg aagacggagg ttggttgctg gtccgctttt
     3421 ccgggaccga gccgattgtc cgagtctact gtgagaccac tcaagagaag cgggtcgagc
     3481 cgatcctcgc agatggctta aggctgctcg gagtgagttg aatcaccggc tgaccgacac
     3541 ccactgccac ctcagcctgc ccgaattctc gggggatctg gatgagaccc ttgagcgggc
     3601 tcaggcgtcc ggcatcggga ccctgatcgt ccctgggatc gatctgccga ccagccgcat
     3661 ggcggtgagc ttggccgaga gccaccccgg gctgttcgcc gcggttggaa tccacccgca
     3721 cgaggcggag agctggactc cagcggccgg ggtggagctg cgcaagctgg ctcggtcccc
     3781 gacggtgaaa gccatcggtg aaatcgggct ggactactat cgcgatttgg ctgctcggcc
     3841 agcgcagcgg gccgccctcc aggcccagct ggagatagcc ggcgacttga ggctcccggt
     3901 gatcgtccac aatcgggcgg ccatcgaaga gctgctggcg gttctgatag aatggtcggg
     3961 cggcctcgat ccaaggctgc ggtcccgagc cggagtgttg cacgcgttct ccggcgagct
     4021 tgaacatgcc caaaccgcaa tcggctcggg ctttttcatt ggagtagctg gcccgctgac
     4081 ttatcctaac gcccaaccgc ttcgagagct cgtgcagcgc
//
LOCUS       DATDPQ010000074         8765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_545327, whole genome shotgun sequence.
ACCESSION   DATDPQ010000074 DATDPQ010000000
VERSION     DATDPQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545327"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..116
                     /locus_tag="VI520_01685"
     tRNA            44..116
                     /locus_tag="VI520_01685"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Trp,seq:cca)
     gene            176..394
                     /gene="secE"
                     /locus_tag="VI520_01690"
     CDS             176..394
                     /gene="secE"
                     /locus_tag="VI520_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HLF36625.1"
                     /translation="MAKAQLARRRSNPIVQLARETTAELRKVSWPTRQEAVQLTLIVL
                     AVVIVSSALLGFLDFLFARVIGFIIALG"
     gene            855..1370
                     /gene="nusG"
                     /locus_tag="VI520_01695"
     CDS             855..1370
                     /gene="nusG"
                     /locus_tag="VI520_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HLF36626.1"
                     /translation="MWYVVHCYSGYENKVRHNLEQRIETMNMKDRIFDVVVPTEEEME
                     VREGKRRTVERRVFPGYILVQMIMNEDSWYVVRNTPGVTGFVGMGNEPTALRPEEVSQ
                     IIRRMEAEAPRIKVTYKTGQKVRIIDGPFNDFIGTVDAIDMDRAKVRVMVSFFGRETP
                     VELDFLQVEKA"
     gene            1399..1824
                     /gene="rplK"
                     /locus_tag="VI520_01700"
     CDS             1399..1824
                     /gene="rplK"
                     /locus_tag="VI520_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HLF36627.1"
                     /translation="MAKKIKTVVKLQITAGRATPAPPIGTVLAPHGINLMAFCKEYNA
                     RTVSQTGEILPAEITIYMDGSFSFILKTPPAAVLLRKAAGVEKGSGEPNRQKVGRVTR
                     DQIREIAEVKLKDLNAIDLEGAMRQIEGTARNMGIEVTE"
     gene            1831..2535
                     /gene="rplA"
                     /locus_tag="VI520_01705"
     CDS             1831..2535
                     /gene="rplA"
                     /locus_tag="VI520_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HLF36628.1"
                     /translation="MAKHGKKYRAAQEKIVPQELYEPRRALELAKETSFSTFDGTIEI
                     HLRLGVDPRHAEEQIREMVQFPHGLGKSVRVVAFAEGDDARLAQQAGADFIADDELLD
                     RIQKGWTDFDATVATPSMMGKAGRLGKILGPRGLMPNPKAGTVVPGQDLPRVIKELKA
                     GRVEFRSDKGANLHVPIGKASFGTEMLYDNMAALMDVVKRARPASAKGNYIRKVVVTS
                     SMGPGVKVDPTAAQAM"
     gene            2771..3304
                     /gene="rplJ"
                     /locus_tag="VI520_01710"
     CDS             2771..3304
                     /gene="rplJ"
                     /locus_tag="VI520_01710"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HLF36629.1"
                     /translation="MAITKPRKQALVAQYQELAGRSKAMILTSYSGLSVKQLEGLRNQ
                     IRELGGEFHIVKNSLMGLAFKELKLPVPEALRHGTTAIGFAVEDVPAIAKMIVDLSRE
                     NPGMRVKAGLIEGSLYDEAQVIRLAELPPMPVVRAQLLGLLTTPASQAAGVLAAALRG
                     LAGVIHAYSQSKSPEAA"
     gene            3361..3741
                     /gene="rplL"
                     /locus_tag="VI520_01715"
     CDS             3361..3741
                     /gene="rplL"
                     /locus_tag="VI520_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HLF36630.1"
                     /translation="MTDLKKLADQLGALTILEAAELTKMLEDQWGVSAAAPMAMLAPT
                     MAAAADAEPAEEQTEFLVVLKDVGPKKIDVIKAIRQITTLGLKDAKDLAETAGAKVLE
                     GASKEASQDAKSKLEAAGATVEVS"
     gene            3871..4551
                     /locus_tag="VI520_01720"
     CDS             3871..4551
                     /locus_tag="VI520_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36631.1"
                     /translation="MREHILVIEDDASILQLLRRGLAYEGYSADTAANGPEGLLKARE
                     RPPDLVVLDWMLPGLDGLEVCRRLRSGGPVPILMLTAKDAVADRVEGLDAGADDYLVK
                     PFDLEELLARVRALLRRAKPEEGSESISFADLRLDTGTHQAFRGSREIALTAKEYELI
                     ELFLRHPRQVLTRDQIYDRVWGYDFGGESNIIEVYVRYLRQKTEAATEPRLIHTVRGV
                     GYVMREES"
     gene            4644..5945
                     /locus_tag="VI520_01725"
     CDS             4644..5945
                     /locus_tag="VI520_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF36632.1"
                     /translation="MLTKQVDDSLLSTASDIRSAAIRTMDGVIGFPSLALNLTAEARV
                     QVWQTDGVLAVQAPGLEELPLDDLAPGLTSAGYRNVILEDVEYRVLTYPLIAQPENRV
                     VGYLQLANSLATVRQALSNLLILMVVGGIVAVALAGLVGWSTAGAALRPLDRVTETAV
                     QITRADDLSRRIPLAGPPRGEVGRLSQAFNETLERLEGLFETQRRFLADVSHELRTPL
                     TTIRGNVDLIRRMKTADPESLEAIKAEVDRMSRMVNDLLLLAQAESGRLPLAMEPIEL
                     DTLMLEVFKQAKVLASNGVRVEIGREEQVQVRGDKDRLKQVFLNLVANALDHTGSGGV
                     VTLSLGCVEGWARLTVSDTGRGMPQEELPHIFERFYRIDPARKRSDLGGAGLGLSIAY
                     WITRSHNGRIEVASEVGKGTSFSVWLPQVGEAAEEPSPSPG"
     gene            complement(5996..6646)
                     /locus_tag="VI520_01730"
     CDS             complement(5996..6646)
                     /locus_tag="VI520_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLF36633.1"
                     /translation="MRAARAVVWLPVVLAASFPIGLLTAWTAALPDRDSAPPPVPPPN
                     ASFVQVNPPISAAFTSEVQYWAPDIGHWSAEFGLPADLIATVMQIESCGHPEVESTAG
                     ALGLFQVMPFHFGAGEDPLLPEVNAARGLSYLARGFELAGGELGLTLAGYNGGHGLIG
                     RPAADWPAETRRYVRWGTQIMTDLREGAVPSPGLAAWLDAGGASLCRRAASALGID"
     gene            complement(6655..7290)
                     /locus_tag="VI520_01735"
     CDS             complement(6655..7290)
                     /locus_tag="VI520_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36634.1"
                     /translation="MSVYAEQLRQVGGSAGRVRPWVGLLRSERDRVFTLIMLAALVTF
                     ELFNFSTTEFALKDLLGGLRLGTAQWATILALAFSAVDFAGVAWLFSPKQGVRVEAWY
                     LVGAWLLAATMNATLTWWSVSLALLQHESLGNEIIARETLLSSAPVFVAGLIWLLRVL
                     LIGSFTLASRRPSGAELQPVVATAAIAPSAPTDRSGRSVRRTLIARPIRQR"
     gene            complement(7416..8363)
                     /locus_tag="VI520_01740"
     CDS             complement(7416..8363)
                     /locus_tag="VI520_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HLF36635.1"
                     /translation="MTEKPTPSGAFPRLFISPDERRLRAGWRLLGHSALLLILGVVFS
                     VVLAIPLAVFQFVSRQNLTNAILALSTVVSLPAITLATWIARRGLDRRSFRSLGLELG
                     TGWSRDLLVGFAIPIPLFGLIYLFELGAGWLDFQSWGWAERGVLATAVGVLLLLLAFV
                     AVGFYEELLVRGYYLQNLKDGTNLVVAVFATSAAFGLAHLGNPNASWTSTLGIFAAGL
                     FLAYAWLRTRALWLPIGIHIGWNFLQGPVFGFEVSGIPTPSLVRHVVNGPELITGGAF
                     GPEAGLLVLPAMALGAGLIWWYTRHRVTAVGSPSAAEQG"
     gene            complement(8402..>8765)
                     /locus_tag="VI520_01745"
     CDS             complement(8402..>8765)
                     /locus_tag="VI520_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF36636.1"
                     /translation="QPTIVSEVEVSHRLWKHEMFLPVTMVHAIESLEQGMQLANDVSY
                     GLTAGFYGSEQEAEWFFDHIEAGVTYANRPQGATTGAWPGFQPFGGWKGSGSSGKNAG
                     GVHYLQLYMHEQIHTLIR"
BASE COUNT         1862 a         2573 c         2836 g         1494 t
ORIGIN      
        1 ggatcggaaa cacactctgc accgcgaaac gaagtagaac cgaaggccag tagctcaatt
       61 ggtagagcac cgctctccaa aagcgggggt tgcgggttca agtcctgcct ggcctgcctg
      121 attgatcacg gcgtcgaata attgaggcgc cggggttggc cagaggagaa tccgattggc
      181 aaaggcgcaa ctcgcccgcc ggcggtcaaa tccgatcgtc cagctggcgc gggagaccac
      241 cgcagagctg agaaaagttt cctggccgac ccggcaggag gcggttcaac ttactttgat
      301 agtgctggcg gtcgtcatcg tgtcgagcgc attgctgggt ttcctggatt ttctatttgc
      361 ccgggtgatc ggcttcatta tcgccttggg gtgaaggaat cgggatagtg gaagaaaaga
      421 acgagcagga gccgcaggtg acaggcggag cgagcgctga agctgtggag actgccgcct
      481 cggaagaggc agagcttgag ctgcgcccgg atttggaaac cgagtcagcg gacgaagcgc
      541 ggcctcctga gcctgaccag gcggcagacg aagccatgag cgagggctat ccggcccctt
      601 cggtcgaagc cgaatcagcc gagcaagtgc ttccccccgc cactccgatc caagaactgc
      661 agggcccgga gtcggcggct tctcctgagc ttgactccgc ggtggaggaa gtcggagctg
      721 aagagacgga gctgccggct gaaccaactg gggccccgga aatctcggac gccgggtcgg
      781 acggcgcttt gaaagcgccc gaaccgaccg aggccctcgc cgaagtcagc cacgctgagc
      841 cggatgacgg acggctgtgg tacgtggttc actgctattc cggatacgag aacaaggttc
      901 gtcacaatct cgaacagcgg atcgagacga tgaacatgaa ggacagaatc tttgatgtag
      961 tcgtcccgac tgaggaggaa atggaggttc gggagggcaa acggcggacg gtcgagcgaa
     1021 gggtctttcc gggctacatc ctggtgcaaa tgatcatgaa tgaggactct tggtacgtgg
     1081 ttcgcaacac gccaggggtg accggatttg tcgggatggg caatgaacca accgcactcc
     1141 ggccggagga agtctcccaa atcatccgca ggatggaagc ggaggcgccc cggatcaaag
     1201 tgacctacaa gaccggacag aaggtccgga tcatcgacgg tccattcaac gacttcatcg
     1261 gcaccgtaga tgccatcgac atggaccggg ccaaggtccg agtcatggtc tcattcttcg
     1321 ggcgtgaaac cccggtcgag ttggatttct tgcaagtcga gaaggcctga gccaagcatc
     1381 taggagcaga gcgagccagt ggccaagaaa atcaagacgg ttgtgaagct gcagatcacg
     1441 gctggccgag ctaccccagc tccgccgatc gggacggtgc tggctcccca tggcatcaat
     1501 ctgatggcgt tctgcaaaga atacaacgcc cggacggtga gccagaccgg tgagatcctt
     1561 ccggccgaga tcacaatcta catggacggc agcttcagct tcattctaaa gaccccgccc
     1621 gccgcggtgc tgctgcgtaa ggcggcgggg gtcgagaaag ggtcgggcga gcccaaccgt
     1681 cagaaggttg ggagggtcac ccgcgatcaa attagggaaa tagccgaggt caagctcaaa
     1741 gacctgaatg cgattgatct tgagggcgca atgcgacaga ttgaaggcac cgcccgcaat
     1801 atggggatcg aggttacgga gtagcaatca atggcgaaac atgggaagaa ataccgggcg
     1861 gcccaggaga agatcgtccc gcaagagctt tacgagccgc gccgggccct cgagttggca
     1921 aaggagacat ccttttcaac gttcgacgga accattgaaa tccatcttcg gctgggggtt
     1981 gatccgcgac atgcagagga gcagatccga gagatggttc agttccccca cgggctggga
     2041 aagtcggtgc gggtggtcgc cttcgccgaa ggtgacgatg cccgactggc ccaacaggcc
     2101 ggggccgatt tcattgcgga tgatgagctg ctcgatcgga tccaaaaagg ctggaccgac
     2161 ttcgatgcaa ccgtcgccac cccctcgatg atgggcaaag ccggtcggtt gggcaagatc
     2221 ttggggccgc ggggtctgat gccgaatccc aaggccggaa cggttgttcc cggccaggat
     2281 ttgccgcggg tgattaagga actcaaagcc ggcagggtag aattcaggtc ggacaagggc
     2341 gccaatttac atgtgccaat cggaaaggct tcctttggca ccgagatgct ctacgacaac
     2401 atggcggctt taatggacgt tgtgaagcgg gcccggccgg cctcggccaa agggaattac
     2461 atccgcaaag tggtcgtcac cagcagcatg ggcccgggag tgaaggtcga tccaaccgca
     2521 gctcaggcga tgtagctagg agcagataag aactcaatcc gccgacgatc caacctctgg
     2581 actttcagcg ggatcgaagg cggagcgaac cagtcaagct gattcacccg agacagcagg
     2641 cgttccgttc tgtggagctt aatttcctgc cgaggtgaag gacgatctcc gatccgttcg
     2701 gagaggcgag cctttgccga agcgacttgc gacggcaaag gctttttcgt tcgcgtgaaa
     2761 ggataaaaga ttggcgatca ccaaaccacg caagcaagcc ctggtggctc agtaccagga
     2821 gttggccggt cggtcgaagg cgatgatcct gacctcctat tccggcctgt ccgtgaagca
     2881 gctagaaggg ctgcgcaatc agatccgaga gctcggtggc gagttccaca tcgtcaaaaa
     2941 cagcttgatg ggattggcct tcaaagagct caaacttccc gttccagagg cgttgaggca
     3001 cggcacaact gcgatcggtt ttgcggtcga ggatgtacct gccatcgcca agatgatcgt
     3061 ggatctcagc cgagagaacc cggggatgcg agtcaaggca gggctcatcg aagggagctt
     3121 gtatgacgag gcacaggtca tccggttggc tgagctgccg ccgatgccgg tcgtgcgggc
     3181 ccaattgctc gggttgctga ccacccccgc cagccaggcg gccggggtgt tggcggcggc
     3241 cttgagaggg ttggccggtg tgatccacgc ctattcccag agcaagagtc cggaggccgc
     3301 ctgagcggcg atgtcgacaa gtcctaagga cccacaactc taacgaagga gaatgcaaag
     3361 atgactgatt tgaaaaagct tgcggaccaa cttggggccc tcaccatcct ggaggcggcc
     3421 gaactgacca agatgctgga ggaccagtgg ggcgtctcag ccgcggcacc tatggcgatg
     3481 ctcgcgccga cgatggcagc ggctgccgat gccgagccgg ccgaggagca gactgaattc
     3541 ctggtggtct tgaaagacgt tggcccgaag aagatcgacg tcattaaggc tatccgccaa
     3601 ataaccacct tggggctcaa ggacgccaaa gacctggcag agactgccgg ggccaaggtc
     3661 ctggagggtg caagcaagga ggcctcccag gatgcgaaat cgaaactgga ggccgccggg
     3721 gcgaccgtcg aagtgtcctg agcctggctc gcacacaatt tgaatcaaac ggcttctgcc
     3781 gtttgccgcc ggggctgtcc gccatggaca gcccgtgctt ttcccggcac aacccttcag
     3841 ccagccgacg tccgcggtac catagttggg atgcgtgagc acatcttggt gatcgaggat
     3901 gacgccagca tccttcagct gctccgacgt ggactggcct atgagggtta ctcggcggac
     3961 accgccgcca acggcccgga aggcctgctg aaggcccgcg aacggccgcc cgatctggtc
     4021 gtgttggatt ggatgttgcc cggcctggat ggtctcgaag tgtgccgaag gctgcgctca
     4081 ggcggcccag tcccgatctt gatgctcacc gcaaaggacg cggtggccga tcgggtggag
     4141 gggctcgatg ccggggccga tgactacctg gtcaagcctt tcgatctcga agagctgttg
     4201 gctagggtgc gggccctgct gcgtcgggcg aaaccggagg aggggtcgga gtcgatttcc
     4261 ttcgccgacc tgcgcttgga taccggcacc catcaggcct tccggggcag ccgcgagatc
     4321 gcgctcaccg cgaaagaata cgagctgata gagctcttct tgcgtcatcc tcggcaggtc
     4381 ttaactcgag accagatcta cgatcgggtg tgggggtacg acttcggggg cgagagcaac
     4441 atcatcgaag tctacgttcg ctacctccga cagaagactg aggccgccac ggaaccgcgg
     4501 ctgatccaca cggtccgggg cgtgggctac gtgatgcggg aggaatcctg accctgcggg
     4561 cgcggctgac cctctggtac agcggtctgc tggccggcgt cctgttcctg ttcgggaccg
     4621 cagtctatct gctgctctcc tacttgctga ccaagcaggt cgacgactcg ttgctctcga
     4681 ccgcctccga tatccgcagt gccgcgatcc ggaccatgga cggtgtgatt ggattccctt
     4741 ccttggcgtt gaacctcacg gccgaagcgc gagttcaggt ctggcagacc gatggggtgc
     4801 tggcggtcca agccccgggg ttggaagaac tgccgctgga tgacctggct ccaggactga
     4861 ccagcgctgg gtaccgcaac gtaatcttgg aggatgtcga ataccgggtg ctgacctatc
     4921 cgctgattgc ccagccggaa aacagggtcg tcggctacct gcagttggcc aattcgctgg
     4981 cgacggtgcg gcaggccttg agcaatcttc tgattctgat ggtggttgga ggtattgtgg
     5041 cggtggcgct agcaggcctg gtgggctggt ctaccgccgg cgctgccctg cgaccgctgg
     5101 atcgagttac ggaaacggcc gtgcagatca cccgggccga cgacctctcc cggcgaatcc
     5161 cgctggctgg cccaccccgc ggcgaggtcg gccggttgag ccaggcgttc aatgagaccc
     5221 tggagcggct ggagggattg tttgagaccc agcggcggtt tctggccgat gtttcccacg
     5281 agctgcgaac gccgctcacc accattcgag gcaacgtgga tttgatccgg cgaatgaaga
     5341 ccgccgatcc cgagtcgttg gaggccatca aggccgaagt cgatcggatg tcacggatgg
     5401 tgaacgattt actgttgttg gcccaggccg aatcgggtcg cttaccgttg gcgatggaac
     5461 cgatcgagct cgataccctg atgctggaag tgtttaagca agccaaggtc ttggcttcga
     5521 atggcgtgcg ggtggagatc gggcgggaag agcaggtcca ggtgcgggga gataaagacc
     5581 ggctaaaaca ggtgtttctg aatctggtcg ccaacgcgct ggatcacacg ggatccggcg
     5641 gggtggtgac gctctcgctg ggttgtgtgg agggttgggc gcggctgacg gtgtcagaca
     5701 ccggtcgtgg gatgccccag gaggagctgc cccatatctt tgagcgtttc tatcggatcg
     5761 accccgctcg taagcgcagc gatcttgggg gcgccgggct cggactctcg attgcctatt
     5821 ggatcacccg gagtcacaac ggccggatcg aggttgcctc agaagtgggg aaggggacca
     5881 gtttctcagt ctggctgccg caggtgggtg aagccgctga agagccttcc cccagtccgg
     5941 gctgaggccg gagcgccgtc cgaacactgc aacccaggag cagcccgccc gcgaatcaat
     6001 cgatcccgag ggcggaagct gcccgccgac acaggcttgc cccgccggcg tccaaccagg
     6061 ccgcaaggcc ggggctgggt accgccccct cccgaagatc ggtcattatc tgggtgcccc
     6121 atctcacata gcgccgggtc tcggcgggcc aatccgccgc gggccggccg atcagcccat
     6181 gccccccgtt gtatccggct agggtaagtc cgagctctcc gcctgccaat tcaaaccccc
     6241 gggcaaggta actcaagcct ctggcggcgt tgacctcggg cagtagagga tcctcacctg
     6301 cgccaaagtg gaaaggcatg acctggaaga ggcccagcgc gccggccgtc gattccacct
     6361 cgggatgccc acaggactcg atctgcatca cggtcgcgat caagtccgcc ggcagaccga
     6421 attcagcaga ccagtgcccg atatcgggag cccaatactg gacctctgaa gtgaacgccg
     6481 ccgagattgg gggattcacc tgaacgaaac tggcattcgg cggaggcacc ggcggcggcg
     6541 ccgagtcccg gtcgggcagc gcagctgtcc aggcagtcag cagcccgatg ggaaaactgg
     6601 ctgccaggac gactggcagc cagacgaccg cccgcgcggc acgcattccg ggatctatcg
     6661 ttggcggatc ggccgggcga tcagggttcg cctcaccgac ctgccagacc gatcggtagg
     6721 cgccgagggc gctatcgcag cagtcgccac cacaggttgc agttcagccc ctgaggggcg
     6781 gcgggaagcg agggtaaaac tgccgatcag caataccctc agcaaccaga tcagaccggc
     6841 cacaaagacc ggcgcgctgg agagaagggt ctctcgagcg atgatctcat tccccaatga
     6901 ctcatgctgc aggagtgcga gcgagaccga ccaccaggtc aaggtcgcat tcatggtagc
     6961 cgccagcagc caggcaccta ccagatacca ggcctcgacc cggacaccct gtttagggga
     7021 gaacagccag gccactccgg cgaaatcgac cgcgctgaag gcgagggcaa gaattgtcgc
     7081 ccactgggcg gtgcccaacc gcaggccgcc tagcagatcc ttgagcgcga actccgtggt
     7141 gctgaagttg aacagctcaa aggtcaccag ggccgcgagc atgatcaggg tgaatacccg
     7201 atcccgctcg cttcgcagca ggccgaccca cggccgaact ctacccgccg accctccgac
     7261 ttgcctcaac tgctccgcat agaccgacat agcgcacctc ccttagcacg tttgttcggt
     7321 tgcaaaagga tagaacacat gtgctattga gtcaagacgc cagatttgcc gggcaataaa
     7381 agcggagggc tagctgcggg ggaggcggag tgcgctcaac cctgttcggc tgcagatggg
     7441 gaccccacgg ccgttactcg atggcgggta taccaccaga tcaagccagc cccaagggcc
     7501 atggcgggca gcacgagcaa cccggcttct gggccaaacg cgccgccggt gatcagctcg
     7561 ggcccgttca cgacatgcct gaccagggag ggggtcggaa taccgctgac ctcgaatccg
     7621 aaaaccggac cttgcaggaa attccagccg atatggatac caatcgggag ccagagggcc
     7681 cgggtccgca gccaggcata agccaggaag agcccggctg cgaagatgcc gagggtggag
     7741 gtccaagaag cattcgggtt gccgaggtgt gccagcccaa aggcagccga ggtcgcgaac
     7801 accgcaacga ccaggttggt gccgtccttg agattctgta aataataccc tcggaccagc
     7861 agctcctcat agaacccgac cgccacgaag gccagcaaca agagcagcac cccgaccgca
     7921 gtagccagca caccgcgctc cgcccaaccc cagctctgaa agtctagcca ccctgccccc
     7981 agctcgaaga ggtagatcag gccgaagagt gggatcggaa tggcgaaccc gaccagcaaa
     8041 tcgcgggacc agccggtgcc gagctccagt ccgagggatc gaaaggagcg acggtccagc
     8101 cctctccggg caatccaagt cgcgagcgtg atcgctggca acgagacgac ggtgctgagc
     8161 gccagaatgg cgttagtgag attctgcctg ctgacgaact gaaagactgc cagcggtatc
     8221 gcgagcacga ccgaaaacac cacacccaag ataagcagca aagccgagtg gccgagcagc
     8281 cgccagccgg cgcggagccg cctttcatcg ggagagatga acagacgcgg gaacgcaccc
     8341 gacggagtcg gtttctcagt cattggatca tcgcatgctg agcggacgcg ggcgatcgaa
     8401 gctaccggat cagggtgtga atctgctcgt gcatgtacag ctgcaggtag tgcacgcccc
     8461 ccgcattctt gccagaggag cctgacccct tccagcctcc gaagggctga aagccaggcc
     8521 acgcgccggt ggtcgcgccc tggggccgat tggcgtaggt caccccggcc tcgatgtgat
     8581 caaagaacca ctcggcctct tgttcgctgc cgtagaagcc ggcggtcaac ccataactca
     8641 catcgttggc cagctgcatg ccctgctcca ggctctcaat ggcgtgaacc atcgtgactg
     8701 ggaggaacat ctcgtgcttc cacagccgat ggctgacttc cacctcggag acgatcgtgg
     8761 gctga
//
LOCUS       DATDPQ010000075         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1076364, whole genome shotgun sequence.
ACCESSION   DATDPQ010000075 DATDPQ010000000
VERSION     DATDPQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076364"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VI520_01750"
     CDS             <1..1301
                     /locus_tag="VI520_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36637.1"
                     /translation="GLLAACNYPGPAAAPTAESPATTGEALEAASPTPSASPSPLPPT
                     PTPSPSATPPPPFLHIVYVEDNDLWALEPGVPPVRLTESGNVSDVRLASDGELIVYVV
                     TDANFEFVEMRSLGFDGSDDRLLLGAGDLASLYPLEGFLRYTLADFSLAPGTHRVLLN
                     TRGVFEGPGLAKSDDLLALDADRGALTRLLAPGQGGDFTLSPDGTQLAIVRPSSISFA
                     RADGTDLRSEVLSFSLVQTFSEYFYYPRPIWSPEGDSVLVAIPGENPFAESPSGTIRR
                     VPAAGGSPTVLASISGDLFRPQAEAPLISPDGTTVAYLRAGALSGEDELLWYRPGTGE
                     SFTYALGTLQWKGWAPDSVHFAYTAGSGLDLFIGEVGGPPRPLGSGSSLRWVDDQQYL
                     YLVGIPGRWTLTLGSLSAAPLSLVELVGSAGAFVSYDFAR"
     gene            complement(1306..>2084)
                     /locus_tag="VI520_01755"
     CDS             complement(1306..>2084)
                     /locus_tag="VI520_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36638.1"
                     /translation="SNIRQAVQAEIERQIREVEAGRTIESWTLDWDDERGVLSKMRSK
                     ESAADYRYFREPDLLPIRIEEAEKQTLQHALPELPLARRGRLVQGYGLPEYDAEILTE
                     ERSLSDFFETTLSAYGGDPKRVSNWVMNEVLRLIRQAGTTAEELGLKPTHLAEIIQLV
                     DAGMITAGVGKDLLEQARGAEKTPRQMVEEQALAQVTDTDVLRKAAEVILQANPTQVE
                     SYRAGKLTVLSWFVGQLMRETGGRADAKKARELLQELLQR"
BASE COUNT          309 a          723 c          635 g          417 t
ORIGIN      
        1 cgggcttgct ggccgcctgc aactatcccg ggccggcggc ggccccgacc gcggagtcac
       61 ctgccacaac cggcgaggcg ctggaggcag ccagcccgac tccctcggcg agccccagcc
      121 cgcttccccc cacgcctacg ccttctccat ccgccacccc tccccctccg ttcctccaca
      181 tcgtgtacgt tgaggacaac gatctctggg ccctagaacc gggagttccg ccggtccgac
      241 tgaccgagtc cggcaacgtc agcgacgttc ggctggcgag cgatggggag ctgatcgtct
      301 acgtcgtcac ggatgctaac ttcgagttcg tggaaatgcg cagccttggg ttcgatgggt
      361 ccgacgaccg gctgctgctc ggggccggcg acctggccag cttgtacccg ttggagggtt
      421 tcctgcggta caccctggcc gatttcagcc tcgcgccggg tacccaccgg gtgctattga
      481 atactcgcgg cgtgttcgag gggccggggc tggccaagag cgatgacctg ctcgcacttg
      541 acgcggaccg cggcgcactg actcggctgc tcgcgcccgg tcagggcggc gatttcaccc
      601 tctcaccgga cggaacccag cttgccattg tcaggccgtc cagcatcagt ttcgcccgag
      661 ccgacgggac cgacctccgg tccgaggtgc tcagcttcag tttggtgcaa accttcagcg
      721 agtacttcta ctatccacgg ccgatctggt cgccagaggg tgactcggta ctcgtcgcaa
      781 ttccgggcga gaacccattc gcagaatctc cctccgggac gatccggcgg gtgccggcgg
      841 ccggcggctc cccgaccgtg ctggcctcga tctcaggcga cctctttcgg ccccaagccg
      901 aggccccgct gatctcaccc gatggaacga ccgtggccta cctgcgagcg ggagcgttga
      961 gcggcgagga tgagctgctg tggtaccggc ccggaaccgg ggaatctttc acctacgcct
     1021 tggggaccct ccagtggaag ggttgggccc ccgattcagt tcacttcgct tacaccgcgg
     1081 ggagtggtct ggacctcttc atcggcgaag tcggtgggcc gcctcggccg ctcgggtctg
     1141 gctcgagcct gcgatgggtc gatgaccagc agtacctgta tttggtgggc atccctggca
     1201 gatggaccct cactctcggc agtctttccg cggctccgct gtcgctggtc gagttggttg
     1261 ggtcggcggg agcatttgtc tcttacgatt tcgcccgctg aacggctacc gctgcagtaa
     1321 ttcctgaagc agctcccggg ccttcttggc atcggcccga ccgcccgtct cgcgcatcag
     1381 ctgaccgaca aaccaactta agactgtcag ctttcccgcc cggtagcttt cgacctgggt
     1441 tgggtttgcc tgcaggatca cctcggcagc ctttcgcaag acgtccgtat cggtgacctg
     1501 ggccaaggcc tgttcctcga ccatctgccg gggggtcttt tccgcccccc gcgcctgttc
     1561 cagcaggtcc ttgccgaccc cggccgtgat catgcccgcg tcaaccagct gaatgatctc
     1621 ggccaggtgg gtcggcttca aacccagctc ttcggcggta gttcctgcct ggcggatcag
     1681 ccgcaggacc tcgttcatca cccagttgga tactcgcttc ggatcacctc cgtaggctga
     1741 gagggtcgtt tcgaagaaat ccgagaggct ccgctcctca gtaaggatct ccgcgtcgta
     1801 ttccggcaag ccatagccct ggaccagtcg accgcggcgg gccaacggca gctccggcag
     1861 cgcgtgctgc agcgtctgct tctcggcctc ttcgattcga atcggaagca ggtccggctc
     1921 ccggaaatag cggtagtcgg ctgcgctctc cttggaacgc atcttgctga gcacccctcg
     1981 ctcatcgtcc cagtccaggg tccaggactc gatcgttcgc cccgcctcga cctcccggat
     2041 ctgccgctcg atttcggcct ggacggcctg ccggatattg ctga
//
LOCUS       DATDPQ010000076        10609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2901062, whole genome shotgun sequence.
ACCESSION   DATDPQ010000076 DATDPQ010000000
VERSION     DATDPQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901062"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VI520_01760"
     CDS             <1..485
                     /locus_tag="VI520_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF36639.1"
                     /translation="TREALESVGMSGSEEAQIFDLGKGQRQRLALASVLALRPRILVI
                     DEPTTGQDPTMAQEIFDILKRLNGQGTTVIVVTHKVDLAAAYCSRAVVMAEGQIAFDG
                     SFPELLTDPERMRQLSLEPPETTRLAALLATYGVSPWISTFDQLSEALRILVEPTDGD
                     "
     gene            475..1515
                     /locus_tag="VI520_01765"
     CDS             475..1515
                     /locus_tag="VI520_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HLF36640.1"
                     /translation="MAIEYQKGESTFHRLDARTKLLLFAGLTIVSVLILDPLMIAALF
                     LILYEIGARSIDRKMLNRNLRVLVVIFLTFSAFQIIFFTPNDAHLLFYIVPGRQWLPV
                     TVQGLVRAAAVFFRFFIVVLSVHLLLYTTPPVNLVLALTRRERARRALPTAGISILLG
                     AILFLSFQLGGPQGEPAGGLSRLPLEGAARLAAVAALSAALGYVVYRVASHGLPPDMG
                     IALSLGFATVGVLSQQAQQITDAQKARGYDVRPRNLIRRMQVLASLLMPIFLATLERS
                     QDIAVAILARAFDYNLAKRTYRRELRFHRRDYLVHALIVGLILGGLALKHYGLASPTE
                     DWILSKFAASEP"
     gene            1727..2653
                     /locus_tag="VI520_01770"
     CDS             1727..2653
                     /locus_tag="VI520_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLF36641.1"
                     /translation="MADHYLDDSVTQPFWDNSVTPRLEVESGDVIAMDIPESCNQVTP
                     IWTDDDLAHISFDPIHALIGSIYVRGAEPGDALQVDILKMEHKGWGWSGHLQGFGLLA
                     DDFPNAYIHHWKLEGDWCRFGVQDIRLPFEPFFGTIGVAPKEPGRINTIPPRANAGNV
                     DIRNLGPGARVWLPVFVPGALVSLGDCHSAQGDGEVCGTGVESPMHAAVRLSVRKDLA
                     VQELQFSTPGPMNKTDTQGYHATTAHGPDLMENAKNAVRHMIDWLVETHDLSRSQAYV
                     LCSTAGDLKISEVVDAPNWIVSFYMPRSIFAG"
     gene            2811..5264
                     /locus_tag="VI520_01775"
     CDS             2811..5264
                     /locus_tag="VI520_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36642.1"
                     /translation="MKPSPRTRTILLPRRAAVSFLVFVVVAFLSLPALADHIGPHRTV
                     PSFDWERLACHYEAVYDPPGTGYFGCTLELFEPPDGSCPSEGSVAGFFNPGACSGWPG
                     GCTTLPCDISLDDSIQGCSEGEEGCRAVEKTSELPPATVNGSISCSVSGSGGWCRGGA
                     ELSLSGSEPLSGYSILSLEGTRNGEAFGCEGAACGVPLLEGNNSFTFWARSSYGDTSS
                     AGSADGNLDSAPPTISGDATGSAGDADWFVSEVTVSASASDAAPGSGLASLEVSVDGG
                     GWAAYSGPIVLSDGEHIVDLRASDGAGNGAQESLEVRVDTQPPAADLSAGSSFCPGCG
                     QTLGIDLSVQDAGRGIVEWTLKAGGVLVASGSGPTSQNLGWDGSGLGGGSHDLTLAAR
                     DEAGNSAEASDTFDLIVPTAEPEPPPEEAAAESTSPPTSATPIGTASTGTAAATASIQ
                     PTRTPLTIPFGALPAAPLPSGGQEPLPNVSSGSSGESSSTASESTSGPVFGAAALALI
                     ATATAFTLDRLRRRQQEEAALRLEMERRNAAAEAKDEVDRAALAAAIAAASVAASELD
                     RLSAEEAQAKWMDQLLADSRPDATDPAAVASSVSPEGDGEKALLTVAPDGETEQRSRA
                     TASSVVADALRNLFPSFRFTPHYTEEPLINTGLIEAGAYEHWGARTELNPVVELAPER
                     WVAAPFGVRIGVGWDAGRISISFNTPTTVHDLGEGGTTASVRGSGAAALTWNGWNTRL
                     EARYSPLDFSVGNEGTVGYSGSGSQGVYVREMPVQGLVLLGGIALVGWGLATMNPQVP
                     QWIERLKQILTPGGLAPVY"
     gene            complement(5288..5971)
                     /locus_tag="VI520_01780"
     CDS             complement(5288..5971)
                     /locus_tag="VI520_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36643.1"
                     /translation="MNSNSHRRRRIRLGCILAILPPLLAAGCCILLARPRDPGKVVEA
                     ARQFQSEWQSRFQGLNEDQVEAVAVEVVGGSVQFGLVLGKIQTDRDAAGLTLFDADLG
                     NFGEYPVVRPRVVLRLKDEQGRLVETVTLTGQSATLGPGMAERVHGEFDPKGTPPRIV
                     EEKLTIVSDSFLPIWRYWYDDVRLAPSPLSRIADVLPLVSYEALWTNPMEPWRSYEIE
                     FRVEDILQG"
     gene            6156..6656
                     /locus_tag="VI520_01785"
     CDS             6156..6656
                     /locus_tag="VI520_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36644.1"
                     /translation="MAGRTHLGIITIAGVSGVLGAVVLAVTNALAGEDRVAVLTEVIG
                     LKTGLLLGAGLGALVGGWLAHGGAKRIAGFTGVVLAGLMWVALLLILRPHAWILDAVM
                     EGMLGEDGAAYSPGILVFILILSWRALKRALAGLVYGLLTRQVSARGTGSSDLGASFR
                     SASGDE"
     gene            6839..7273
                     /locus_tag="VI520_01790"
     CDS             6839..7273
                     /locus_tag="VI520_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HLF36645.1"
                     /translation="MNCPKDGTELKHKERHGVEVDSCESCRGIWLDYSELDAIEDTVF
                     AEDDLKGSMIFAKSPTEYKCPICGSPLSQFRYRLHDLTLEYCPNLHGFWLDAEEEERV
                     LDLLREREKDLGRKFKAEAEWSNAIRRLRSPSFAEKIRNLLK"
     gene            7834..8073
                     /locus_tag="VI520_01795"
     CDS             7834..8073
                     /locus_tag="VI520_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLF36646.1"
                     /translation="MNQTDNKGYHATTAHGPDLMENAKNAVRHMIDWLVETHDLTRSQ
                     AYVLCSTAGDLKISEIVDVPNWIVSFYMPRSIFSA"
     gene            8210..9142
                     /locus_tag="VI520_01800"
     CDS             8210..9142
                     /locus_tag="VI520_01800"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HLF36647.1"
                     /translation="MTQIRVDPLQLLESAQQLAGLSAKLAELAEEAGETGRSAPSYEG
                     QFGPQVQAMASELQRIITKQAQRLAELTSDLRIASEGFAKADEESASGIEGLSQALRD
                     WATQRGLLTEDGSLAWAPLSNFLLGLAYPRQPLAQTADQVGPDAGRTPTPTYPAPTPT
                     PSLVQSPTSTPTRTPTATPSATVTPTETPTPSQTPSPQPILPTNTPGTPAVHWQELND
                     RFFTAIDEFIGSYSKIPHADNPPIDYPNDFLTPVDQYPPTAKVAQLARVILTIFATLR
                     DLWEPIKDLDPIYPLDEPVGQGSTDTNLPTRQGS"
     gene            9607..10029
                     /locus_tag="VI520_01805"
     CDS             9607..10029
                     /locus_tag="VI520_01805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLF36648.1"
                     /translation="MNWILVALMSTIALRLLLSISYSLITEGQPSIQTVRPLTAIGLA
                     AAFFSFLGDSAVQEEPVFRGFLWGYLESFGLKQPMIWIVQAALFWIAHLRYLDRPFTF
                     WLALPMAGLLLGWLSWKSKSIAASVLAHAAYNSMASYF"
     gene            complement(10429..>10609)
                     /locus_tag="VI520_01810"
     CDS             complement(10429..>10609)
                     /locus_tag="VI520_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36649.1"
                     /translation="VALRQPTQHQEIDEGDSNEEVGEQEPSDPAYDRMAFFLNGGWCF
                     LHFLLVLVLQVLRRL"
BASE COUNT         1970 a         3449 c         3173 g         2017 t
ORIGIN      
        1 gcacccggga agcgctcgag tcggtcggga tgtccggctc ggaggaggcg cagattttcg
       61 atctgggcaa gggtcagcgc cagcgactgg cgctggcttc ggtcctggcg ctgcggccgc
      121 ggatcctggt catcgacgag ccgacgaccg gtcaggaccc gaccatggct caggagatct
      181 tcgacatcct caagcgactg aacgggcagg gcaccacggt catcgtcgtt acccacaagg
      241 tcgacctggc tgcggcctac tgctcgcggg cggtggtgat ggccgagggg cagatcgcgt
      301 tcgacgggtc gttccccgaa ctgctcaccg acccggagcg catgcgccag ctctcgctcg
      361 agccgcccga gaccacccgc ctggcggcgc tgctggccac ctacggcgtg tcgccctgga
      421 tctcgacctt cgaccagctc tccgaggcgc tgcgcatact ggtggagcca accgatggcg
      481 attgaatatc agaagggcga gtcgaccttc caccggctcg acgcccgcac caagctgctg
      541 ctcttcgccg gcctgacgat cgtctccgtc ctgatcctcg acccactgat gatcgcggcc
      601 ctgttcctga tcctgtatga aatcggggcc cgctcgatcg accgcaagat gctcaaccgc
      661 aacctgcggg tgctggtggt gatcttcctg accttctcgg ccttccagat catcttcttc
      721 actcccaacg acgcccacct ccttttctat atcgtgcccg gcaggcagtg gctgccagtg
      781 accgtccagg gcctggtgcg cgccgcggcg gtgttcttcc ggttcttcat cgtcgtcctt
      841 tcggtgcatc tgctgctcta caccaccccg ccggtcaacc tagtcctggc gctcacccgc
      901 cgcgagcgcg ccaggcgagc gctcccgacg gccggcatct cgatcctgct cggcgcgatt
      961 ctgtttctca gcttccagtt gggcggccca cagggcgagc cggccggcgg actgagtcgc
     1021 ttgccgctag agggtgccgc ccggctggcc gccgtcgccg ctctcagcgc ggcgctcggc
     1081 tacgtcgtct atcgggtcgc aagccacggg ctaccgcccg acatggggat cgcgctgagc
     1141 ctgggcttcg ccacggtcgg ggtgctctcc cagcaggcgc agcagatcac cgacgcgcag
     1201 aaggcgcgcg ggtacgatgt ccggccgcgc aacctgattc gccggatgca ggtgctcgcc
     1261 tccctgctca tgcccatctt cctcgccacc ctcgaacgct cgcaggacat tgcggtcgcg
     1321 atcctggcgc gggctttcga ctacaatctc gcgaagagga cctatcgacg cgagctgcgg
     1381 ttccatcggc gcgactatct ggtccacgct ctgatcgtcg ggctgatcct gggcggattg
     1441 gccctcaagc actacgggct ggcgagcccg actgaggatt ggatcctgag caagttcgcc
     1501 gcttcagagc cctgacatgc cacattccct gcgccgccgc aacccttcac cggatcacct
     1561 cggtgcgccc tgacatgcca ccctccctcc gccgccgcga ccctccacct gttcaccgcc
     1621 ctgcgccctg acatgcccgc cacattcttc tattctttcc gcccgcccgc tcgctcgctg
     1681 ccatacccag tgccagtgat ggctatgcgc aaccaggagt tcgaccatgg ctgaccacta
     1741 cctcgacgac agtgtcaccc aacccttctg ggacaactca gtgacgcccc ggctggaggt
     1801 cgagtccggc gacgtgattg cgatggacat ccctgagtcc tgcaatcagg tcacgcccat
     1861 atggaccgac gacgacctgg cccacatcag cttcgacccg atccacgcgt tgatcgggtc
     1921 gatctacgtc aggggtgctg agccgggcga cgcgctgcaa gtggacatcc taaagatgga
     1981 acacaagggc tggggttgga gcggccacct ccagggcttc gggctgctgg ccgacgattt
     2041 ccccaacgcg tacattcacc actggaagct cgagggcgat tggtgccggt tcggggtgca
     2101 ggacattcgg ctgcccttcg agccgttctt cgggacgatc ggcgtcgcgc cgaaggagcc
     2161 gggtcggatc aacaccattc cgccgcgggc caacgccggc aacgtggaca tccgcaacct
     2221 ggggcctggt gcgagggtgt ggttaccggt cttcgtcccc ggtgccctgg tgtcgttagg
     2281 tgattgccac agcgcccagg gcgacggcga ggtgtgcggg acgggggtgg agtctccgat
     2341 gcacgccgcg gtgcgcctca gcgtgcgcaa ggacctggcg gtgcaggagc ttcagttcag
     2401 cacgccgggg ccgatgaaca agaccgacac acagggctac catgccacga ccgcccacgg
     2461 ccccgacctg atggagaacg ccaagaacgc ggttcgccac atgatcgact ggctggtcga
     2521 gacccacgac ctgagccgca gccaagccta cgtcctgtgc agcaccgccg gcgacctgaa
     2581 gatcagcgag gtcgtcgacg cgccgaactg gatcgtgtcg ttctacatgc cgcggagtat
     2641 ctttgctggc tgatcgcgga ctgccggatc ctcgtcgctc gttcccggct tgttcgcgaa
     2701 atcacaaagt tacgtttcgc aacttaggtt gattccgctt cgaaactccg gtaacatgga
     2761 aaggcttcac ttcgaacgcc tctggagggg gtccaccgac gggggtgacc ttgaaacctt
     2821 ctccgcgaac gcggaccatc ctccttccgc ggcgagcagc tgtctccttc ctcgtcttcg
     2881 ttgtagtcgc ctttctcagc ctccccgcgc tggccgacca cattggaccc caccgaacgg
     2941 tgccgagctt tgattgggag cggctggcct gtcactacga ggccgtctat gatcctccgg
     3001 gcaccggata cttcggctgt acattggagc tattcgaacc tccggatgga agctgcccat
     3061 cggaagggag cgtggccggt ttcttcaatc cgggggcctg ctctggctgg cctggcggct
     3121 gcacgacttt gccctgcgac atctcactcg acgactcgat tcagggctgc agcgagggcg
     3181 aagaaggctg ccgggcggtc gagaagacga gcgagcttcc ccctgcgacg gtcaatggat
     3241 cgatctcctg ttccgtctcc ggatccggcg gctggtgccg gggcggcgcc gagctctcgc
     3301 tctcgggcag cgagccgctc tccggttaca gcatcttgag cttggaaggc acccgtaacg
     3361 gcgaggcgtt cggctgcgag ggcgcagcct gcggcgtgcc gctgctggag ggcaacaact
     3421 ccttcacctt ctgggcccgg tcgagctacg gcgacacctc ctccgccggg tcagccgacg
     3481 gcaatctcga cagcgccccg ccgaccattt ccggcgatgc gaccgggtcg gcgggcgatg
     3541 ccgactggtt cgtgtcagag gtgacggtct ccgccagcgc gagcgatgcg gcgccggggt
     3601 cggggctggc aagcctggag gtgtcggtcg atggcggcgg atgggcagcc tacagcgggc
     3661 cgatcgtcct ctccgatgga gaacacatcg tcgaccttcg agctagcgac ggcgccggga
     3721 acggcgccca ggaaagcctg gaggtccgcg tcgataccca gccgcccgcg gccgacctga
     3781 gcgccggatc gtcgttctgc ccgggctgcg ggcagacctt gggcatcgat ctgagtgttc
     3841 aggacgcagg aagagggatt gtcgagtgga cgctcaaggc cggtggagtc ctggtggcga
     3901 gtgggagcgg tccgaccagc cagaacctcg gctgggacgg atccggcttg ggaggcgggt
     3961 cacatgacct aaccttggcg gcgcgggacg aggcaggaaa ctcggccgag gccagcgata
     4021 ccttcgacct gatcgttccc accgcggaac cggagccgcc cccggaagaa gccgccgcgg
     4081 aatccacatc gccgcctacg agtgccactc caatcgggac cgcctccacc ggcacggccg
     4141 ccgcaacggc ctccatccag cccacccgca cgccgcttac catcccgttc ggggctctcc
     4201 ccgccgcgcc gctgccttcg ggcggacaag agccattgcc gaacgtatcc tccggttcct
     4261 cgggcgaatc cagctcaacg gcgtcggaga gcacctcggg tccggttttt ggagcggccg
     4321 ccctggccct catcgccact gcgaccgcct tcaccctcga ccgcctccgg cgccgtcagc
     4381 aagaggaggc cgcgctccga ttggagatgg agcgccgcaa cgcggcggcc gaagctaaag
     4441 atgaagttga ccgggccgcc ctcgccgcgg cgatcgcggc ggcctcagtc gctgcttctg
     4501 agctggaccg cctcagcgca gaggaggccc aagcgaagtg gatggaccaa ctcctcgcag
     4561 attcgaggcc ggatgcgaca gatcccgcgg ccgtggcatc atcagtatca ccggaaggag
     4621 atggtgagaa ggcccttctg accgtggcgc ccgatggcga gacggaacaa cgctcccggg
     4681 caaccgcttc atccgtcgtc gccgatgcgc tcaggaatct gtttccgtcg tttcggttca
     4741 cgccgcatta caccgaggaa ccgctgatca acacaggcct cattgaagca ggagcctacg
     4801 agcactgggg agcgcggact gaactcaatc ccgttgtaga gctcgcgccc gagaggtggg
     4861 ttgcggctcc gttcggcgtg aggattggcg taggctggga tgccggcagg ataagcatct
     4921 cattcaacac gccgacgacc gtgcacgacc taggcgaggg tgggactact gcttccgtgc
     4981 gaggcagcgg cgcggcagct ctcacctgga atggctggaa taccagactt gaggcgcgat
     5041 actcgccgct agacttttcg gtcggaaacg agggtacggt tggttattcc ggcagtgggt
     5101 cgcaaggggt ttacgtcagg gaaatgccag tgcaagggct cgtgctcctt ggcgggatag
     5161 ccctggtcgg ctggggccta gcaacgatga atcctcaggt cccgcagtgg atcgaacgcc
     5221 tcaagcagat tcttacgccg ggcgggctag ccccggtgta ctaggccagc aacccgggtc
     5281 ccgcgagcta cccctgcaga atgtcttcca ctcggaactc gatctcgtac gatcgccacg
     5341 gctccatcgg atttgtccat agcgcctcgt acgaaaccaa cgggaggaca tcggcaatgc
     5401 gagagagcgg gcttggggcc agtctaacat catcgtacca gtagcgccaa atgggaagaa
     5461 agctgtcaga cacaatcgtg agtttctcct ccacaatcct ggggggagtc cccttcgggt
     5521 cgaactcgcc gtggacgcgc tcggccattc cggggccgag cgtggctgac tggcctgtga
     5581 gggtaactgt ctcaaccagc cggccctgtt catccttaag ccgcagcacg actcgcggtc
     5641 gtacgacggg atactcgcca aaattgccca gatcagcatc gaacaaggtc agccctgccg
     5701 catcgcggtc tgtttggatc tttccaagca ccagcccgaa ctgaaccgag ccgccgacga
     5761 cctcgaccgc cacggcctca acttgatcct cgttcagtcc ctggaaccta ctctgccact
     5821 cggattgaaa ctgccgggcc gcctcgacaa ctttccctgg atcgcgcggc cgagccagca
     5881 gaatgcaaca gccagccgcc aggagcggag gcaagatagc cagaatacaa cccagtcgga
     5941 ttcgccgcct ccggtgagaa ttagagttca ttggttttcc aacgagggcc gagtgtgatg
     6001 cagattatag atgagatgta cgcccggcac taaggcctat gcggcagcct accaatgcgc
     6061 acaatcgtgg tgccgaatac cctgggaggg gtccgttgtc ggttgaacca gcatgatcga
     6121 taccgctggt ccagacggac cccgctaatc ggccaatggc agggcgaact catttgggaa
     6181 tcataacgat cgccggggtt tccggtgtgc ttggggcggt cgtgctcgcc gtgacaaatg
     6241 ccttggctgg cgaagatcgg gtggcagtcc ttaccgaagt catcggtctc aagacgggac
     6301 ttctgctcgg tgccggcctg ggtgcgttag tcggaggttg gttggcacat ggcggtgcca
     6361 agcggatcgc agggttcacc ggcgtggtcc tggctggcct catgtgggtg gccctactct
     6421 tgatccttcg accgcacgcc tggatcctgg acgcggttat ggaaggtatg ttgggagagg
     6481 atggagccgc atactcaccc ggaatcttgg ttttcatcct gattctctca tggcgtgcac
     6541 tgaaacgtgc attggccggg ctagtctatg ggctacttac ccggcaggtt tccgcccggg
     6601 gaactggctc ctcggacctg ggcgcttctt tcagatccgc ttccggagat gagtagacct
     6661 tggttcaggc ggtactcaac ctgcgaacct catgtccggt gaggagcttc gatccaatcc
     6721 aaatcagagt gaaatcggtc catgattacg gccgctgtca gagcgagctg gtactgcctc
     6781 ggccggtttg catcaatcac ggtttgcagg tatgttacgc tccgctagga gcacagcgat
     6841 gaattgcccg aaagacggaa cagagcttaa gcacaaagag cgccacggag tcgaggtcga
     6901 ttcctgcgaa agctgtcgag gcatttggct ggactactca gaactcgatg cgatcgaaga
     6961 tacggtattc gcggaggacg atctcaaagg ttcgatgatc ttcgccaaga gcccgaccga
     7021 gtacaagtgt ccgatttgtg gatcgcccct cagccagttc cgatatcggc ttcacgacct
     7081 aactttggag tattgcccta acctgcacgg cttttggctc gatgcggagg aggaagaacg
     7141 ggttctcgat ctgcttcggg aacgagagaa ggacctaggg cgcaagttca aagcggaggc
     7201 ggagtggtcg aatgccatcc gcaggttgag gtccccgagc ttcgccgaaa agataaggaa
     7261 cctgttgaaa tagcattatc ctaagctcct tcgagggccg ctcaaaccct tgactcatga
     7321 tccaggtctg cctgccagcg ccggttcgct acaccgcctt gagccactcc tagaggcgag
     7381 gcgccttgga tcaagcgcga gaatcgaagt tccccatccc gttgtcgaac tagccgacct
     7441 ggcaaaatcc ttgagccccc accgaccctc gcccttcaca ggccggggtc ggttcctgat
     7501 gcgagatacg gactgaacgt ggagagccgc gcgacatggc catgatcgtc cacctccacc
     7561 acgggaggac gacccggtcg acgggcggct gctgctgatc cgagtcgccg atggcccgag
     7621 cccccaggcc cgattcgcgg gtgagagggc cctccagctc gggctacggg aagtgaagga
     7681 agagcgaatc acgaataatt cgatctcctc tccctcctct ccgcaggatc cggcacaccg
     7741 gcgtgggcgc aaggggctgc tcatcgggct cggcgtcacg gcgggcgcaa ggacctggcg
     7801 ctgaaggagc ttcagttcag gacgccgggg ccgatgaacc agaccgacaa caagggctac
     7861 cacgccacga ccgcccacgg ccccgacctg atggagaacg ccaagaacgc ggtccgccac
     7921 atgatcgact ggctggtcga gacccacgac ctgacccgca gccaggccta cgtcctgtgc
     7981 agcacggccg gcgacctgaa gatcagcgag atcgtcgatg taccgaactg gattgtgtcg
     8041 ttctacatgc cccgaagtat cttctccgcc tgatttggcg gcagcgccga gccgaaaacg
     8101 ccgcgattct ttcccggcgc tccgggccgc acctataccg tcgggcctgc acaccttgca
     8161 ggttacaatc ctcgcagcct ttgccaatca ctagcctaat cccacgaaga tgacccagat
     8221 ccgggtcgac ccgctacagc tcctcgagtc cgcccagcag ttggccggcc tctccgccaa
     8281 actggctgag ctcgcggagg aggcaggtga aacgggcagg agcgcgccga gctacgaagg
     8341 gcaatttgga ccgcaagtcc aagcgatggc cagcgagctg caacgcatca tcaccaaaca
     8401 agctcaacga ctggccgaac tgacttcgga tcttcgcatt gcttccgagg ggttcgccaa
     8461 agcggacgag gagtctgcat ccggaatcga aggactgagc caggctttgc gagactgggc
     8521 gacccagcgt gggctgttaa ctgaggatgg gtcgcttgcc tgggctccac tatcgaactt
     8581 tctgctcggc ctagcgtatc cgcgacaacc gctggcgcag acggcagacc aggtcggtcc
     8641 cgatgccggc cggactccaa ccccaaccta tccagcgcca acgccgacgc cttcccttgt
     8701 tcaatcgccg acgtcgactc ctacgcgcac ccctaccgcc acaccatcag ctactgttac
     8761 cccgaccgag acgcctacac cgtcccagac gccatctcct caaccgattc ttcccacaaa
     8821 caccccagga acacctgcgg tccactggca agaacttaat gatcggttct tcactgccat
     8881 cgatgaattc ataggctctt acagtaagat cccgcatgct gataacccgc cgatagacta
     8941 ccccaacgat ttcttgactc ctgttgatca gtaccctccg actgcaaagg tcgcccaatt
     9001 ggctagggtg atcctgacaa tctttgcaac cctacgtgac ctctgggagc cgattaaaga
     9061 ccttgatcct atctacccgc ttgacgaacc ggttgggcaa ggatcaactg acacgaatct
     9121 tccaactcgg caaggatctt gagtgaattg agtcgacctg aagcaacggg tgatggaccc
     9181 ggccctagga tccgggtcga ggggaacgaa ccacggactg ctggagatcc tcgtcgcggt
     9241 ccaaccgccc ggcgcctcgc gtggctgctg ctcctctatc tcgtaatccg aatccctctg
     9301 atcaaccttc tggattgggt gggaccgacc gagagtggcc tccccgagac agccctccag
     9361 gtcgcaatgt atgccttgac cgccgcaatg attgccgtaa gccgcgcgtc actaggcgct
     9421 tacagaattg accgcctttc gctcgtactc ttcttggtcg ccggaatcgt gttcgcggtc
     9481 ccgcttccaa cgagcgatag cacaagccag aaccttgtgt tttgcgctta tggtctcgtt
     9541 gcgccggccc ttgcctacct gatttacagg tcgagacact cgatcaagaa ttgggcgccg
     9601 acgagcttga actggatctt ggtcgcactg atgtctacga tcgccctccg cttgcttcta
     9661 tcaatctcct actcccttat caccgaaggg cagccgtcaa ttcaaaccgt taggcctttg
     9721 acggccatcg gactcgctgc cgctttcttt tccttcctgg gcgattctgc agtacaggag
     9781 gagcctgttt tccgagggtt cctgtggggc tacctcgaga gcttcggact taagcaaccc
     9841 atgatctgga tcgtgcaagc tgctctattc tggattgctc acctacgcta cctcgacaga
     9901 cccttcactt tttggctcgc acttccaatg gccggtctgc tcttgggttg gctctcttgg
     9961 aaatcaaagt caattgcggc cagcgtactc gcgcatgccg cctacaattc catggcttcc
    10021 tacttctagt agacaacttc gcttgttcgg ctttgcggcg acctgtggct ctgtcacctt
    10081 ttagcaaact gaaaggggac gtcgacgccc cgaactggtt tgtgtcgttt gcgccctagg
    10141 acgcgcaacc caagggggca acccagccag gtggcgccgg acgccaactc caacataccc
    10201 ggcgccaacc ccgacgtctt ccattgtcca agcgccaacg tcgtctccta cgccgacccc
    10261 gaacgcgacg cccgtgggat ccctgctgcg gtggccgcgc tcagagacct tcttaggccc
    10321 cttgaagatt tagatcccat ctaccctctt ggcgatcccc tgccgcccgg accaagcggc
    10381 ttggaagatg atggtgacga tgtaacatga cgggtattgg ggcccgtctc aaagacgcct
    10441 caatacctgc aagactagaa ctaacaggaa gtgcaggaag caccagccac cattcaagaa
    10501 gaacgccatc cgatcgtagg ccggatcgga tggctcctgc tcgcctactt cctcattcga
    10561 atccccctcg tcaatctctt ggtgttgggt gggttgacgg agagctacg
//
LOCUS       DATDPQ010000077         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3688513, whole genome shotgun sequence.
ACCESSION   DATDPQ010000077 DATDPQ010000000
VERSION     DATDPQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688513"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VI520_01815"
     CDS             complement(<1..869)
                     /locus_tag="VI520_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36650.1"
                     /translation="MSEALFASSLASHLAVAIAFVLLFGVATALPPRPITLRWSAAAV
                     PIVGLRLTLLTLLLVNSWGIFDTGLLVSRLQWFAAAATVLATGWVVLLPQPHPRWDSW
                     LLAITTALVVGLSVSVGIDQLVGTSWLVGLDGYFGTELLRTGEALVYPFVSLLIVRRL
                     IRLTVEQPMPRRVAELGATRMTRPEPAEAWADKFSHEIGEVAGPSPTPAVPEQVETLK
                     AELRLALEELAEVSSQTDPQEPIPVGSLTALIESMRDPLASIQGYGQMLAGGGIGELG
                     PDQRKFVDRINDST"
     gene            complement(943..1953)
                     /locus_tag="VI520_01820"
     CDS             complement(943..1953)
                     /locus_tag="VI520_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HLF36651.1"
                     /translation="MRRLLLIVLFVLGAVFLTTRFDEFLQVLQTLQTGDPRWLGLAIL
                     VHLVWILNTAGSFKAIYRALGIDEQLRHLALVTAAVQFVSVVAPSAGMGGMAILLADG
                     RKRGLPVGRLTTGAALFVFGEQLSLLAFVGLGLLVLFQLNHLSAAEVLAALILGATAL
                     VSAALLALGMESPDQLTRGLYWAGERLNRLIRPLIRRDLFDLSHAHRLAHDVHEGLQE
                     VRRTPQALLLPAALAVSSKFLLLMVLALVFLAFGQTPFAGTLLASFSVGYLFYIVSFT
                     PSGIGFVEGAMTLVLTSLGVPFGRAAVITLAFRGITFWLSILYGMVAVRWIGLEARPE
                     PQ"
     gene            complement(1958..>2540)
                     /locus_tag="VI520_01825"
     CDS             complement(1958..>2540)
                     /locus_tag="VI520_01825"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HLF36652.1"
                     /translation="MKAAGVRSPGGMAAVLGLELEAVVAACEEAQAATGEAVQVANDN
                     CPGQLVISGSKPALASAIERLRANGARRVVELAVSIAAHSALMEPAQAELNRAIESTP
                     ISAPAIPVIGNVNAAVLGSVADIREELRSQLTSRVRWTETIRAMLEAGVSQFLELGTR
                     DVLSNLVKRIDRQVDTAAAGDPAGIERLIRPQN"
BASE COUNT          518 a          824 c          804 g          394 t
ORIGIN      
        1 gtactgtcgt tgatacgatc aacgaacttg cgttgatccg gccccagttc gccaatcccg
       61 ccgccagcca gcatctgtcc atagccctga atcgaagcga gcgggtctcg catgctttcg
      121 atgagcgcgg tcaaggatcc gaccggaatg ggctcttgag ggtcggtctg gctgctgacc
      181 tctgccagct cctcgagggc gagccgcagc tccgccttga gggtctcaac ttgctccgga
      241 accgcggggg taggcgaagg tcctgcgacc tctccgatct cgtggctgaa cttatcggcc
      301 caggcctcgg ctggctcagg ccgggtcatg cgagttgccc cgagctcggc gacccgtcga
      361 ggcattggct gttccacggt taatctgatc aacctcctga cgatcaggag cgagacgaac
      421 gggtagacga gtgcctcgcc cgtcctcagc agctcggtgc cgaagtagcc gtctagccca
      481 accagccagg aggtgcccac cagctggtcg atgcctaccg aaacgctgag cccgaccacc
      541 aatgcggtag tgattgccag cagccaggag tcccagcggg ggtgcggttg cggcaagagc
      601 accacccaac cggtggccaa cactgtggcc gcagccgcga accactgaag cctggagacc
      661 aacagcccgg tgtcaaagat cccccatgaa ttgacgagca gcagagtgag gagcgtcagc
      721 cgcaggccga cgatcggcac cgccgcggcc gaccagcgaa gggttatggg gcgtgggggg
      781 agtgcggtcg cgaccccaaa gagcaggaca aaggcgatgg cgaccgccag gtgagaggcc
      841 aggctactcg cgaacagcgc ctcggacatg ggatggtccg accggaagtc ggccggggat
      901 tatagcacgc gggcccggat gagccttttg agggcgattc gtttactggg gctccggacg
      961 ggcctcaagg ccgatccagc gcactgcaac catcccgtaa aggatggaca accagaaggt
     1021 gatcccgcgg aaggcaagcg tgatcacggc cgcccggcca aacgggaccc caagggaggt
     1081 cagcaccagg gtcatggccc cttcgacgaa tccgatcccg gacggcgtga aagagacaat
     1141 atagaacaga tagcccacac tgaaactggc cagcagggtg ccggcgaagg gggtttggcc
     1201 gaaggccagg aaaaccagtg ccagtaccat gagcagcaga aacttgctgc tcactgccag
     1261 ggctgccggg agcaagagcg cctgcggggt gcggcgcacc tcctggagcc cttcatggac
     1321 atcatgggcg aggcggtggg cgtgggaaag atcgaataga tctctccgaa tgagcggcct
     1381 gatcaggcga ttcagcctct cgcccgccca atacagcccg cgagtcaact gatcggggga
     1441 ctccattccc agagccagca acgccgcgct gaccagggcg gtcgccccaa ggatcagtgc
     1501 ggccaacact tccgcagcgc tcaggtgatt gagctgaaaa aggactagca gacccagccc
     1561 gacgaatgct aagaggctca gctgctcgcc gaagacgaac agcgcggctc cagtggtgag
     1621 ccgcccgacg ggcaaccctc gcttccttcc atcggctaac aggattgcca ttccccccat
     1681 cccggcgctc ggggcgacca cgctgacgaa ctggaccgca gccgtcacca gggctagatg
     1741 ccggagctgc tcgtcgattc ccagtgctcg atagattgcc ttaaaggaac cggccgtgtt
     1801 caagatccag accaggtgca ccaatatggc caggccgagc cagcgcgggt caccggtttg
     1861 cagcgtttgg agcacctgca agaactcgtc gaagcgcgtg gtgagaaaga cggccccgag
     1921 cacgaagagc acgatcagca ggagcctgcg catccggcta attctgcggg cggataagac
     1981 gctcgatccc ggccggatcg ccggctgcgg cggtgtcgac ctgccggtca atccgtttca
     2041 caaggttaga cagcacatcc cgcgtcccta gctcgagaaa ctgactcaca cccgcttcca
     2101 gcatcgctcg aatcgtctcg gtccatcgca cccgggaggt cagctgggag cgcagttctt
     2161 cccggatgtc cgcgaccgag ccaagcacgg ccgcgttcac gtttccgatg actgggatcg
     2221 caggtgccga aataggggtg gactcgatcg cccgattcaa ctcggcctgg gccggttcca
     2281 tcaacgcaga gtgggcggcg atgctcaccg ccaactcgac gacccggcgg gccccgttgg
     2341 cgcgcagccg ttcgatggcc gaggccagcg ccggcttgga tcctgagatc accaactgtc
     2401 ccggacagtt atcattggca acctggacgg cttcgccggt cgcggcctgg gcctcctcac
     2461 aggcggccac taccgcctcc agctccagcc cgagcactgc cgccattcct cccgggctgc
     2521 gaactccagc ggccttcatg
//
LOCUS       DATDPQ010000078         3120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_808465, whole genome shotgun sequence.
ACCESSION   DATDPQ010000078 DATDPQ010000000
VERSION     DATDPQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808465"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VI520_01830"
     CDS             complement(<1..447)
                     /locus_tag="VI520_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HLF36653.1"
                     /translation="MSENGHTLIEIPQSLTVRELADRIEASPIDVIKQLMANGVMANI
                     NQQIDFDTAAIVIAEFGFEALPLKAPEAEAGVEQAELPHWRRMLGDEDPGSLVTRPPV
                     VTILGHVDHGKTTLLDAIRQTEVAAGEAGGITQHIGAYQVSHNDRSI"
     gene            complement(496..774)
                     /locus_tag="VI520_01835"
     CDS             complement(496..774)
                     /locus_tag="VI520_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HLF36654.1"
                     /translation="MSPRTRKHIPQRTCVGCREVLAKRSLVRVVRTPQGVRIDPSSKA
                     QGRGAYVHDRRECWEAAFKGSLARALRVELSETDRRELEMYVSTLPIP"
     gene            complement(790..2724)
                     /gene="nusA"
                     /locus_tag="VI520_01840"
     CDS             complement(790..2724)
                     /gene="nusA"
                     /locus_tag="VI520_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLF36655.1"
                     /translation="MKSEFSLAFNEITERFGLSRDTVMEALQAAMVSAYRRSVNASTA
                     QAIEARIASDTGEVEIFAEKEIVDAVENPLTEVSLVEALRHDPNAEMGGMTLVESTPK
                     DFGRVAAQTAKQVILQRLREAERDAQYEEYVEREGDLVHGTVHSITPQAVTIGLGRAE
                     ALLPRNQQIPGERFHVRDRVRVFVLEVRKSSRGPVIVVSRTHPNMLRRLLEMEVPEIN
                     QGLVEIKAIAREAGKRAKVAVAATRDGVDPVGACVGLRGVRIQSIVRDLNDEKIDVIE
                     WNAESAAFIAKALSPARVSGVFLDDDPERGKTAVVVVPEDQLSLAIGRAGVNARLSAR
                     LTNWRIDIKSLVEAATEALNRLATEESYADMAAAEAEILPQVKAILAKKEETRPVTPE
                     EYHQLTAFVTRVEQEVAARRVSERLRIQEKLELIRQRVPVGAYQTELAVTGLSNRVLK
                     LLTEAGYQSAGQVLEQMELDPDKILALQGIGPKAIVEIGEKLRSIEYPEPLPPEVLVE
                     QASAEPVVAMEPGSEVPAAEPGAEVLLLPASAGDTLVPAEAQPAGIEPEGVPVAQPLE
                     KVFEEAVELLQGAVVEPDEEEEREGAEVVLEDAEKPKKAKKRAKIVEYDPDLDTTVVR
                     HTHKSGRELDWENLDEPEKS"
     gene            complement(2881..>3120)
                     /locus_tag="VI520_01845"
     CDS             complement(2881..>3120)
                     /locus_tag="VI520_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36656.1"
                     /translation="GRPSCEGGTATGTHFHLARRYNGEWMPADGAVPFVLDGWVAKQG
                     NALYEGTLLKGSNVVAACTCSTRANRILYELPGGG"
BASE COUNT          534 a         1012 c          892 g          682 t
ORIGIN      
        1 gatcgaacga tcgttgtggc tgacttggta ggcgccgata tgctgggtga tgcctccggc
       61 ctcccccgcg gcgacttcgg tctggcggat tgcatctagc agggtggtct ttccatggtc
      121 gacatggccc aggatcgtca ctaccggggg acgggtcacc aacgaaccag gatcttcgtc
      181 cccgagcatt cgccgccaat ggggaagctc ggcctgctcg actccggcct ccgcctccgg
      241 cgccttcaag ggcagggcct cgaacccgaa ctcggcgatc acaatcgccg ccgtatcgaa
      301 atcgatttgt tgattgatgt tggccatcac gccattggcc atcaattgtt ttataacatc
      361 gatggggctg gcttcgatcc ggtccgccag ttcgcggacg gtgaggctct gcgggatttc
      421 gatcagggtg tgtccgttct cactcacatc gcacctccag ctgctcggcc gcgacggagc
      481 gagtagcccg gcggcctagg ggattggcaa cgtacttacg tacatctcca attcgcgtcg
      541 atcggtctcg gaaagctcga cccgcagcgc ccgtgcgagc gaacctttga aggcggcctc
      601 ccagcattcg cgacggtcat gcacataggc cccccgtcct tgggccttgg aactcgggtc
      661 aatcctcacc ccctgcgggg tgcggacgac tcgaaccaga gatcgctttg caagcacctc
      721 ccggcaccca acacaggtcc gctgggggat gtgttttctg gtcctaggag acacgatcgg
      781 gcaacgatct caggattttt cgggctcgtc cagattttcc caatccaact cgcgtcccga
      841 tttgtgcgtg tgcctgacta cggtcgtgtc cagatccgga tcatactcca cgatcttggc
      901 gcgtttcttg gccttcttgg gtttttccgc gtcctccagg accacctccg ccccttcccg
      961 ctcctcctcc tcatcaggtt ccaccactgc gccctggagc agctctaccg cttcctcgaa
     1021 gaccttttca agcggctggg cgacgggaac tccctccggc tcgatcccgg ccggctgcgc
     1081 ttcagccggc accagggtgt cccctgcact ggccggcaac agaagcactt ccgcacccgg
     1141 ctcggccgct ggtacttcgc tgccgggctc catggccact accggttcgg cgctcgcttg
     1201 ctcgaccagg acttctggcg gcagcggttc gggatattca atggacctca gcttttcgcc
     1261 gatttccacg atcgccttcg ggccgatccc ttgcagggct aggatcttgt ccggatcgag
     1321 ctccatttgt tccaagactt gaccggcgga ttgataaccg gcctcggtca acagtttgag
     1381 cactcggttg ctcaggccgg tcacagccaa ttcggtttga taagcaccga ccggaactcg
     1441 ctggcggatc agttccaatt tttcctggat ccggaggcgc tcgctgactc ggcgggccgc
     1501 gacctcttgt tccacccggg tcacgaaagc ggtgagctgg tggtactcct cgggcgtaac
     1561 cggacgggtc tcttctttct ttgccaggat cgccttcact tgcggcagga tttcagcctc
     1621 ggcagccgcc atgtcggcat acgactcttc ggtcgccagc cggttcagcg cctcggtcgc
     1681 ggcttcgacc aagctcttga tatcgatccg ccaattcgtt agtcgagctg aaagccgagc
     1741 gttgactcct gccctgccaa tcgccaggct cagctgatcc tccggcacaa cgactactgc
     1801 ggttttgcct cgctcgggat cgtcatccag aaagacgccc gatacccgag ccggactcag
     1861 tgccttggcg atgaacgcgg ccgactcggc attccattcg atgacatcga ttttctcgtc
     1921 gttgaggtcg cgcacgatgg actgaatccg gacgcctcgc agaccgacgc aggccccgac
     1981 cggatccact ccatctcggg tcgcggcgac cgctactttg gcgcgtttgc cggcttcccg
     2041 ggcgattgcc ttgatctcga ccaaaccctg gttgatttcc gggacttcca tctccagcag
     2101 acgtctcagc atgttggggt gggtccgcga aacaacgatc actggaccgc gggaggactt
     2161 gcggacttcg agcacgaaga ctcgcacccg atcccggaca tgaaagcgtt ctcctggaat
     2221 ctgctggttg cgcggcagaa gcgcttcggc tcggccgagg ccgatcgtga ctgcctgggg
     2281 tgtgatcgag tgtacggtgc cgtgcaccag atctccctca cgctccacgt attcctcgta
     2341 ctgagcgtcc cgctcggcct ctcgcaatcg ctgcaagatc acttgcttgg cggtctgggc
     2401 tgctacccga ccaaagtcct taggagttga ttcgaccaga gtcattccgc ccatctcggc
     2461 gttcgggtcg tggcgaaggg cttcgaccag cgaaacttcg gtgagtggat tctcgacggc
     2521 gtcgacgatt tccttctccg cgaagatttc aacttcgccc gtgtcggagg caattcgagc
     2581 ctcgatcgct tgagccgtcg aggcgttgac ggagcggcgg taggccgaca ccatcgcggc
     2641 ttgcagggcc tccatcaccg tgtcacggga gagaccgaag cgttccgtga tttcattgaa
     2701 agccaacgag aattcactct tcatgctaca tcccctgctt ttgttcgcct tggaggcaca
     2761 gcgagcgggt gcatctagcc cattattaag aaaagtgggg gttgcccact tttcggaacc
     2821 acaacttcag gccgaacacc ttaattctag cacaggccgg ttacgactgc aagtagccgg
     2881 ctagcctcct cccggcagct catagaggat ccggttcgcc cgggtcgagc aggtacaagc
     2941 ggcaactacg ttggatcctt tgagcaaggt tccttcgtac agcgcattcc cttgcttggc
     3001 gacccagccg tcgagcacga aaggcacagc cccatcggcc ggcatccact ctccgttgta
     3061 gcggcgcgcc aggtggaagt gagttccagt ggccgtgccg ccttcacagg atgggcgccc
//
LOCUS       DATDPQ010000079         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3425355, whole genome shotgun sequence.
ACCESSION   DATDPQ010000079 DATDPQ010000000
VERSION     DATDPQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3425355"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /gene="polA"
                     /locus_tag="VI520_01850"
     CDS             <1..1674
                     /gene="polA"
                     /locus_tag="VI520_01850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLF36657.1"
                     /translation="YVPVGHLPDLSGARQLDLSTVITALRGPLTDAKIEKVGHNLKFD
                     FTVLARYGLEADPLSFDTMLAEWLLNPSSHSLGLKNLAWVRLGIEMTPIADLIGTGSK
                     QRTMAQVPIAEVAPYAAADAEVCLRLRKVLEKELTRKEQGSLFYDLEMPLVRVLADME
                     MAGVALDTKFLAKLSKELAARVETLETKLFKLVGHPFNVSSTQQLSQVLFDELGLKPA
                     DGSRRTAAGQYSTAADVLESLREEHKVVELILEHREISKLKSTYTDALPEQLNPTTKR
                     VHTSYSQAGAVTGRLASANPNLQNIPIRTELGRKIRRAFIAEPGNTLLAVDYSQIELR
                     VVAHIAVDKTMIKAFHEDQDIHAATAAAVFGTTLKKVTPEQRRSAKAVNFGLIYGMGA
                     FGLTRATDLTLAEAQKFIDVYFERFPGVRAYLARAREQAAEQGYVETLLGRRRYFPQL
                     TKGGQPGPDQVRARAEREAVNSPIQGSAADIIKLAMLELPDALRTAKLKARMLLQVHD
                     ELVFEVPTAQLKRTAKVVQQVMQSAYKLKVPLKTDAKAGPNWEQMEPIG"
     gene            1803..>2950
                     /locus_tag="VI520_01855"
     CDS             1803..>2950
                     /locus_tag="VI520_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF36658.1"
                     /translation="MAGREDLFNESMRLGHSAAWDLQWNRAIEFYRKALAEFPNHANA
                     LNSLALALLETGQLDEALLVYKTASQASPEDPIPVEKCAEIYERLGKIKESIQAREDS
                     ADMYIRRKDAGKAVDNWRHIARLDPRNLAARTRLALTYERLGRTRDSAIEYLSVASIL
                     ASGGKIDRAKEAVQRSLAILPGDPDATRYLRLLNQGKKLPAPPQPRGGTAPLRMAQVQ
                     EYLRKDVDQPLEADEQLKDPETAAQGEALTILAGILFEEPKDNGKEQGLGMARIASGQ
                     TAELKPSSAIYRYLAQAIDLQTRGHKEKAVKEFTRAMETGLEHPAAHYNLAMLLKELG
                     KSDEARKHLVASLGHPGLALGANLALGRLAKDKNDIREATRYLLQALK"
BASE COUNT          605 a          963 c          904 g          478 t
ORIGIN      
        1 tacgtgccgg ttggccatct tccggacctc tccggagccc ggcagctcga tctgtcgacc
       61 gtgataaccg cgctgcgcgg tccactcacc gatgccaaga tcgagaaagt cggtcacaac
      121 ctcaagttcg actttacggt ccttgcccgc tacgggctcg aggccgatcc gctcagcttc
      181 gacaccatgt tggccgagtg gctgctcaat ccctcctctc acagcctggg gctcaagaat
      241 ctggcctggg tccggctcgg gatcgaaatg accccgatcg ccgatctgat tgggaccggg
      301 agcaagcaac ggacgatggc ccaggtcccg attgcagagg tcgctcccta tgccgcggcg
      361 gacgccgagg tctgccttag gctccgcaag gtcttggaga aggagctaac ccgaaaggaa
      421 cagggctcgc tgttctacga ccttgagatg ccgctggtcc gggtgctggc cgacatggaa
      481 atggcggggg tcgccctgga cactaaattt ctcgccaaac tttccaagga attggccgct
      541 cgggtggaga ccctcgagac caaacttttc aagctggtcg gacacccctt caacgtgagc
      601 tccactcagc agctctctca ggtgctattc gatgagctgg gcttgaagcc ggcggacggc
      661 tcgaggcgca ccgcagccgg tcagtactcg accgccgccg atgtactgga gtcgctgcgc
      721 gaggaacaca aggtcgtgga gctgatcctg gagcaccggg agatctccaa gctgaagtcg
      781 acctataccg acgcccttcc ggagcagctc aatcccacaa cgaagcgggt gcacacctcc
      841 tatagccagg caggcgcggt gaccggccgg ctcgcctcgg ccaacccgaa tttacagaac
      901 attccaatcc gcaccgaact ggggagaaag atccggcggg ccttcatcgc cgagcccggc
      961 aacaccctgc ttgccgtgga ctactcccag atcgagctgc gggtggtggc ccacatcgcg
     1021 gtcgacaaga ccatgatcaa agcttttcac gaagatcagg acattcacgc ggccactgcc
     1081 gcggcggttt tcggcacgac gttgaagaag gtgacccctg agcaacgccg cagcgccaag
     1141 gcggtcaact ttgggttgat ctatggaatg ggtgccttcg gcctcacccg ggccaccgat
     1201 ctgaccctgg ctgaagcgca aaagtttatt gatgtgtact tcgagcgatt ccccggtgtc
     1261 cgggcctacc tggctcgggc tcgcgagcag gcggcggagc agggctatgt cgagactctg
     1321 ctcggtcggc ggcgctactt cccgcagctc accaagggcg gacagcccgg tcccgaccaa
     1381 gtgcgggcca gggcggaacg ggaggcagtg aattcgccaa ttcagggctc ggcggccgac
     1441 atcatcaagc tcgccatgct tgaactgccg gacgccctgc gtacggccaa actgaaggcc
     1501 aggatgctgc tgcaggtcca cgacgagctg gtctttgagg ttccgacggc ccagctcaag
     1561 aggaccgcca aagtggtcca acaggtcatg caatcggcct acaagctcaa ggtgccattg
     1621 aagaccgacg ccaaggccgg ccccaactgg gagcagatgg agccaatcgg ctgaagctcg
     1681 gccggccgcg gcccacagga cgaccgaccc gaactccctc gtaggcacgg atttccgcgc
     1741 accagatcgg cctggcgcgg tgctcttacg gctcctgtgc tagaatcgat gcccaatttg
     1801 caatggctgg acgcgaagat ctcttcaacg aatccatgcg cctggggcac tcggcggcct
     1861 gggacctcca atggaaccgg gcgatcgagt tctaccgcaa ggcactggct gagtttccta
     1921 accacgccaa cgcgctgaat agcctggcgc tggccttgct cgagaccggg cagctggacg
     1981 aggccctgct cgtgtacaag accgcctccc aggccagccc ggaagatccc atcccggtcg
     2041 aaaagtgcgc cgagatctac gagcgcctgg gcaagatcaa agagtccatc caggcgcggg
     2101 aggactcggc cgacatgtac atccgccgca aggacgccgg caaggcggta gacaactggc
     2161 gccacatcgc ccggctcgac ccgcggaacc tggcagcccg cacccgcctg gctctgacct
     2221 atgaacgcct tggtcggacc cgcgactcgg ccattgagta tctgtcggtg gcttcaatcc
     2281 tcgccagcgg cggcaaaatc gaccgggcca aggaggccgt ccaacgttcg ttggcgatcc
     2341 tgcccgggga tccggacgca acccgctacc tgcggttgct gaaccagggc aagaagttgc
     2401 ctgctccccc gcagccccgc ggcggcaccg ccccgctgcg catggcccag gtccaggagt
     2461 acctccggaa agatgtcgac cagccactgg aggccgacga gcaattgaag gaccctgaga
     2521 cggcggctca gggcgaagcg ctaaccatcc tggccgggat cctcttcgag gagccaaagg
     2581 acaacggaaa agagcagggg ctcggcatgg ccaggatcgc aagcggccag acggccgaac
     2641 tcaaaccttc ctcggcgatc tatcgctatc tggcccaagc catcgacctg cagacccgcg
     2701 gccacaaaga aaaggcagtc aaagagttca cccgggcgat ggaaaccggg ctagagcacc
     2761 ccgctgccca ctacaacctg gcgatgctgc ttaaggagct gggcaagagc gacgaggccc
     2821 gcaagcatct ggtggcctcg ttggggcacc ctggcctggc cctgggcgcc aacctggcct
     2881 tgggacggct ggccaaggat aagaacgata tccgggaggc cacccgctat ttgctgcagg
     2941 ccctcaaatt
//
LOCUS       DATDPQ010000080         2531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3170271, whole genome shotgun sequence.
ACCESSION   DATDPQ010000080 DATDPQ010000000
VERSION     DATDPQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170271"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..697)
                     /locus_tag="VI520_01860"
     CDS             complement(77..697)
                     /locus_tag="VI520_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HLF36659.1"
                     /translation="MGTSAVPDIVVGRLPLYLRALSQMESEGKEITSSQELGEKLGIS
                     SAQIRKDLSQFGEFGKQGTGYGIGYLTEQLKRILRVNQNWDVALVGVGDLGRAVANYA
                     GFLDRGFRVVAIYDNDPRKIGLKVGPFVIRDAAKLAQDLREQRIRVAMLAVPVDEAQS
                     VTDRLVEGGVRAILCYAPTSISIPRSVRVQYIDPVLHLQRMTYYIP"
     gene            829..2088
                     /gene="obgE"
                     /locus_tag="VI520_01865"
     CDS             829..2088
                     /gene="obgE"
                     /locus_tag="VI520_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLF36660.1"
                     /translation="MFLDEVTITVVSGKGGNGIVHFRREKYIPRGGPDGGDGGKGGDA
                     VLICSRTQSTLSAFRHKRRFAAAVGRPGGGSNRTGRSGDELLIPVPAGTIVRDAELGQ
                     LLGELIEPGDRLVVGRGGRGGRGNARYTSSTNQAPRMAERGAPAEVRRLRLELRLIAD
                     VGIVGVPNSGKSTLLAAVTNARPEIGDYPFTTLQPQLGVADLDDETSLVLADIPGLIE
                     GASAGAGLGTGFLKHISRTRALIHLLDGTAADPLADFNQVNTELELFDPVLARKPQVV
                     GLNKLDQPEVRARLPELEAAFKSRGLQLRPVSALSRQGVRELVLEAYRLAQETEVEPE
                     QDLPIYRPKPNGSKYQISRALDGSWRIEGTAIERAAEMTYWEYEEAVRRFQRMLTKIG
                     VTESLREAGAKDGDTVYIGDHELEWRE"
     gene            2085..>2531
                     /gene="nadD"
                     /locus_tag="VI520_01870"
     CDS             2085..>2531
                     /gene="nadD"
                     /locus_tag="VI520_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HLF36661.1"
                     /translation="MIGVFGGTFDPPHIGHLILADEARGVLSLSAVLWVVTGIPPHKP
                     SQPVTPVSHRIGMVERSTHGNPQFEISRLELDRPGPHFAVETLELLAAAYPTERWAYV
                     MGEDSLADLPNWHRPERFVQLCQSIVVLRRSQVEADLDRLERQIPGL"
BASE COUNT          470 a          789 c          813 g          459 t
ORIGIN      
        1 ggcgcctacc gcgggctcag catgctcgaa cggctgcgcc ggaccctgcc gacagtctaa
       61 ctcagctagt cttgttctag gggatgtagt acgtcatccg ctgcaggtgc agcaccgggt
      121 caatgtattg gacccgcacc gagcggggaa tgctgatcga ggtgggggcg tagcacagga
      181 tagcccgaac gccgccctca accagccggt ccgtgacgga ctgggcttcg tccaccggga
      241 cagccagcat cgcaactcgg atccgctgct cacgcaggtc ttgagccagt ttcgccgcgt
      301 cccggatcac aaacggacca accttgagcc cgatcttccg tggatcgttg tcgtagatcg
      361 caacaacccg gaagccccga tcgagaaacc cggcgtagtt ggccactgct cggcccaggt
      421 cgccgacccc gaccagcgcc acgtcccaat tttggttgac tctcaggatg cgcttgagct
      481 gttcagtcag gtagccgatg ccgtaaccgg taccttgctt gccgaactcg ccgaactggg
      541 ataagtcttt gcggatctgg gccgacgaaa tccccagctt ctcgccgagc tcctgcgagg
      601 aagtgatttc ttttccttcg gattccatct gggaaagtgc ccgcaggtaa agcggcaggc
      661 ggcctactac gatgtctggc actgcggagg tccccatttg ggcccgtctg gatgacgttc
      721 ggcaaatcta tcatagccca accataagtg caaactggcg caaatgagct cttccaaacc
      781 gcacaggtat aatccaccga cgctccgggc aagcaataga aatttgcgat gtttctcgat
      841 gaggtcacga ttaccgttgt gtccggcaag ggagggaacg gcatcgttca cttccgtcgg
      901 gagaagtaca tcccgcgagg cggtccggac gggggggatg gaggcaaggg cggcgatgcg
      961 gtgctcattt gctccagaac ccaaagcacc ctttccgctt ttcgccacaa gcgtcgcttc
     1021 gcggcagcag tcggccggcc cggtggtggc agcaatcgga ccgggcgcag cggggacgag
     1081 ctgctgattc cggtgccggc tgggacgatt gtgcgcgacg ccgagctggg ccagctgctg
     1141 ggcgaactga ttgagccagg ggaccggctc gtcgtgggcc gaggaggccg gggcgggcgg
     1201 gggaatgccc gctataccag ctcaaccaat caagcgccgc ggatggccga gagaggcgcg
     1261 ccggcggagg tgcgccggct gcgactcgag ctgcgtttga tcgccgacgt cgggatcgtg
     1321 ggtgtaccca attccggaaa gtccactctg ctggccgccg tgaccaatgc ccgaccggag
     1381 atcggagatt atccgttcac caccctccag ccccagctgg gggtggccga cctcgatgat
     1441 gagacctcgc tggtgctggc cgacatccct ggcctgatcg agggggccag tgctggcgcc
     1501 ggtctgggga ccggcttcct caaacacatt tcccgaactc gggcgcttat ccacctgctc
     1561 gacgggaccg cggcggaccc gcttgccgac ttcaatcaag tcaataccga gctggagctg
     1621 ttcgacccgg tgctcgcccg aaagcctcaa gtggtcggcc tcaacaagct agaccaaccg
     1681 gaggtcaggg cccgcttgcc cgagctggag gccgcattca aatcccgcgg cctgcagctc
     1741 cggcccgtgt cggcgctttc ccgccagggt gtgcgggaac tggtcttgga ggcctaccgt
     1801 ttggcccagg aaaccgaagt cgagccagag caagaccttc caatctaccg gcctaaaccg
     1861 aacggatcca agtaccagat ctctcgtgcg ctggatggca gctggcggat cgaggggacc
     1921 gctattgagc gggcggcgga aatgacctac tgggagtatg aggaagccgt gcgccgattt
     1981 cagcggatgc tgaccaaaat cggcgtgacc gagtcgctgc gggaagccgg tgccaaagac
     2041 ggcgacacgg tgtatatcgg cgaccacgaa ttggagtggc gggagtgatc ggagtgttcg
     2101 gggggacctt cgatccgccc catatcggtc acctgatcct ggcggatgag gctcgcggcg
     2161 tcctgtcact gagcgccgtc ttgtgggtgg tcaccggcat tcccccgcac aaacccagtc
     2221 aaccggtcac cccggtgtcc catcggattg gcatggtcga gcgcagcact cacgggaatc
     2281 cgcaattcga gatctcccgg cttgagctcg atcgccccgg gccacatttc gcggttgaga
     2341 cgctcgagct gctcgccgcg gcatacccga ccgagcgatg ggcctatgtg atgggagagg
     2401 attcgcttgc cgacctgccc aactggcacc ggcccgaacg gttcgttcag ctctgccagt
     2461 cgatcgtcgt cttgcgccgg tcccaggtcg aggccgatct cgaccggttg gagcgacaga
     2521 ttccgggact g
//
LOCUS       DATDPQ010000081         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_548315, whole genome shotgun sequence.
ACCESSION   DATDPQ010000081 DATDPQ010000000
VERSION     DATDPQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548315"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3190)
                     /locus_tag="VI520_01875"
     CDS             complement(<1..>3190)
                     /locus_tag="VI520_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLF36662.1"
                     /translation="PNMILILQAGRIMYANPASEEILGYRRSELLAPEFDYQALSAPE
                     WADELKLRLAQQLEGGELEPFEFQIVCKNGQLADVLQISRLINYQGKVAILLIVSDIT
                     SRKRSEQEREVLLLAEQEQRQLAETLARVGLSLSADLELPSLMDLICLQSMHLFKVGG
                     AYVWMVEGSELVGFAGHGASRAAFIGRRLPLTDPGSLAARVIRERRPMYVNQAESSNL
                     VNPQLMKEFGTKAILGMPLMVGGRAVGALMLLDTENEKRFGEHDIELAGVFGSHLAVA
                     VENARLIEETQRRLRYETLLKEAIGVFTSTLEVKAVLGRLAEKLCQSVDATSAYICDY
                     DRDTKSGKVLAEYYTEDSSAEERQSDLGATYDLPTEFPGRLAAMEAGRALVRQVGDPT
                     LDEVERKHMGAFGAKATLAIPVKVGGQLIAYATIWESRWDRSFTGEEIELCQAIAQQA
                     AIAIESGRLYERAQREIADRQRAQEALRESEERYRAVAETAFTGITLLDTENRMTFVN
                     PAFAELLGYQAEALLGVSLADLMEPDQFAKVRDQARINERGERSQYELVLRRRDDRTR
                     CVLASAAPMNNARGEHIGSVMVVTDISEIRATEEALSVAMVRMEEGLHQTRELIETEQ
                     ALRDTTAAVSGTLNLDEVLDQILENVGRVVGHDTADIMLLIDTEDGKREVRAVRGRGY
                     AERGLSEWLMQLRVPWEEMQNFRKMEKTGKPIAVPDTRTFPGWVGLPETSWIGSYAGA
                     PLRRKGKTFGFLNLSSEFPGTYQQAHAERLQAFADQAAIAIENARLYAQAQAEIVERT
                     RAQAALRVSEQRYRSVFDGVQDAIFVASLTGQIVEANRQACELLGSDRDQVLKCNVRE
                     LFPNDQEALIPTNPGPGTRPLSPTETVARRPDGSAFEVEITSRLHGVGSETLVLVVVR
                     DITERKIAQAAERGFAKMKEEFVLSASHSLRTPLHTLKGYLELLGSGKVREPERAAEF
                     LANAMENAQRIEKIVHELLDVAQMESEAGQIRLENVPVGDLIGSSFESLAGMAQERGI
                     RLVFENDRPHLRVAADRRRLSRAIGEVVENAI"
BASE COUNT          554 a         1073 c          906 g          657 t
ORIGIN      
        1 ttgattgcgt tctcgacaac ttcaccaatg gcccggctca agcggcgccg atctgcggcc
       61 accctcaaat ggggtcggtc attctcgaag accagccgga tcccgcgttc ttgcgccatg
      121 cccgccaggc tttcgaaact cgaaccgatc agatccccga ccggcacatt ctccagtcgg
      181 atttgaccgg cttccgactc catctgcgcg acgtcgagca gttcgtggac gatcttttcg
      241 atccgctggg cgttctccat tgcgttcgcc aggaactccg ccgcccgttc cggctcgcgc
      301 accttcccgg atccgagcag ttcgagatag cccttcaggg tatggagtgg agtgcgtaag
      361 ctgtgggagg cactgagcac gaactcttcc ttcatcttgg cgaagcctcg ctccgccgcc
      421 tgggctatct tgcgctccgt gatatcgcgc accacaacca ggaccagggt ctcgctgccg
      481 acgccatgca gccgggaggt aatctcgacc tcgaacgcac tcccgtccgg gcgccgggcc
      541 actgtctcag tcggtgacag ggggcgggtc cctggccccg ggttcgtggg gatcagggcc
      601 tcttggtcat tcggaaatag ctccctgacg ttgcatttca agacctggtc tcgatcagag
      661 ccgagcagtt cacacgcttg ccgattggct tcgacgatct ggccggtgag actggccaca
      721 aagatagcgt cctgcactcc atcgaagacg ctccgatagc gctgctccga aacgcgcagc
      781 gccgcctggg cccgtgtccg ctccacgatc tccgcctgag cctgtgcata cagccgcgca
      841 ttctcaatcg cgatggcggc ctgatcggca aacgcctgca gccgttcggc atgggcctgc
      901 tgataggtgc ctgggaactc gctgctgagg ttcaagaagc caaaggtctt cccttttcgg
      961 cgcagcggcg cgccggcgta agaaccgatc cagctggttt ccggcagtcc cacccagccc
     1021 gggaaggtcc gggtatccgg cacggcaatc ggtttcccgg tcttctccat cttgcggaaa
     1081 ttctgcatct cttcccaggg tacccgcagc tgcatcaacc attctgagag gccgcgctcg
     1141 gcatacccgc ggccgcgcac tgccctgacc tcgcgcttgc cgtcctcggt gtcgatcagc
     1201 agcatgatgt cagcagtgtc gtggccgacg acccgaccaa cgttttcaag aatctgatcc
     1261 aacacctcgt ccaagttgag cgtgccgctc actgccgcgg tcgtgtctcg cagagcctgc
     1321 tcggtctcga ttaactcgcg agtttggtgc agcccctctt ccatgcggac catcgcgacc
     1381 gagagggctt cttcggtcgc cctaatttcg ctgatgtcgg tgacaaccat gactgaccca
     1441 atgtgctcgc cgcgggcatt gttcatcggc gcggctgagg ccagaacgca ccgggtccgg
     1501 tcgtctcgtc ggcgcaggac caactcgtat tggctacgtt caccgcgctc gttgatgcga
     1561 gcctggtccc gcaccttagc gaactggtcc ggttccatca ggtcagccag cgagaccccc
     1621 agcagagcct ctgcctggta gccgagcagc tcggcgaagg ccggattcac gaaggtcatc
     1681 cggttctcgg tatccagcag ggtgatgccg gtaaaggcag tttcggccac cgctcgatag
     1741 cgctcctcgc tctcccgcag cgcctcctgc gcacgctgcc gatcggcaat ttcccgttgg
     1801 gcccgctcgt acagccttcc gctctcgatt gcaatggccg cctgttgggc gatggcctga
     1861 cacagctcga tctcttctcc ggtaaagctt cggtcccagc ggctctccca gatcgtggcg
     1921 taggcaatca gctgaccgcc caccttgacc gggatggcca gggttgcctt ggccccgaag
     1981 gcccccatgt gcttgcgctc gacttcgtcc agcgtcggat cgccgacctg ccggaccagg
     2041 gcccggccgg cctccatcgc ggccagcctc ccgggaaact cggtcggtag atcgtaggtg
     2101 gcccccaggt cggattggcg ttcttcagcg gaagagtcct cggtgtagta ttccgccaaa
     2161 accttccccg atttcgtatc ccgatcgtag tcacagatat aggcgctcgt ggcgtcaact
     2221 gactggcaca gcttttccgc aagccggccc agcaccgcct tcacctctag ggtggaggtg
     2281 aagacgccaa tcgcctcttt gagcaaagtc tcatatctaa gtcgccgctg ggtctcttca
     2341 atcaaccgag cattctcgac cgctactgcc aggtggctcc caaatacacc ggccagctcg
     2401 atatcatgtt cgccaaagcg cttctcattt tcggtgtcca gcagcatcag ggcgcccacc
     2461 gcccggccgc caaccatcag cggcataccc aggatcgctt tggtgccaaa ctccttcatc
     2521 aactgcgggt tgaccagatt ggaactctcg gcttggttta cgtacatggg gcggcgttcc
     2581 cggatgaccc gggccgcgag tgaccctgga tctgtcagcg gcagccggcg gccaatgaac
     2641 gcggcccgac ttgcgccatg gccggcgaaa ccaacgagct cgctcccctc gaccatccat
     2701 acataagccc cgcctacttt gaacaaatgc atgctttgca ggcagatgag atccatcagg
     2761 ctgggcagct cgagatcggc actcagagac agcccgactc gggccagggt ctctgcaagc
     2821 tgacgctgct cctgttccgc aagcagcaac acctcccgtt cctgttcgga ccgcttgcgg
     2881 ctggtgatat ccgatacgat cagcaggatc gccactttgc cttggtagtt gatgaggcgt
     2941 gagatttgca ggacatccgc aagctggccg ttcttacaca cgatctgaaa ctcgaacggc
     3001 tcgagctcgc caccctccag ctgctgggcc agccgcagct tgagctcgtc ggcccattcg
     3061 ggtgcgctaa gcgcctggta gtcgaactct ggcgccagca gctcggatct ccggtacccc
     3121 aggatctctt cgctggctgg gttggcatac atgatcctgc cggcctgcaa gatcaagatc
     3181 atgttgggtg
//
LOCUS       DATDPQ010000082         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2379629, whole genome shotgun sequence.
ACCESSION   DATDPQ010000082 DATDPQ010000000
VERSION     DATDPQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379629"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VI520_01880"
     CDS             complement(<1..666)
                     /locus_tag="VI520_01880"
                     /inference="COORDINATES: protein motif:HMM:NF017793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HLF36663.1"
                     /translation="MTAPRIRADYEMLRQVQQQFKQQSGACGQSLRKMHQAKDALEGG
                     DWIGEGAKAFYREMNSEILPSWARLVAALDSAAQSASKMSQLIQQAEDEAARLLKLGG
                     STAAASGTGGSTSGAGTTGSINFGTNADQAAVSDHSRQVLQDIMKEAKLDSVTITSTA
                     RTPERQAIAMFDNIESQGVASQKALYGPNGDQVIDVYVAEKAAGKSQAEIISAMEAKI
                     NELG"
     gene            complement(716..874)
                     /locus_tag="VI520_01885"
     CDS             complement(716..874)
                     /locus_tag="VI520_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLF36664.1"
                     /translation="MQAFSPSYLRIRVAYIGGARKRPAEFKKRLAHFIKRTEQNKQFG
                     FGGIDKYY"
     gene            complement(983..1414)
                     /locus_tag="VI520_01890"
     CDS             complement(983..1414)
                     /locus_tag="VI520_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF36665.1"
                     /translation="MKLRVGDPWMSSKAYSHTLKSLSINLLVAQIERALAFQREVLGA
                     SVVYSDPDFAVCSGFGTEWMLHADHTYDKHAMGGTVAPGTPRGGGVEIRLHGCDPDQA
                     EAAARRLGHEVLVPTTDKGHGMREVYLRDPDGYIWVPDVAI"
     gene            complement(1442..2515)
                     /locus_tag="VI520_01895"
     CDS             complement(1442..2515)
                     /locus_tag="VI520_01895"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HLF36666.1"
                     /translation="MGPAYRPGFNPLTGLPVSDPQTLLTVPLLVSITEFPPSARPQSG
                     LSAASQVWETSIGEGMSRFLAVYYGDYQEVLSDLLESHPKLTTSDPAIGPIRSGRVGY
                     QEIKLFYPGALLIIRFGSPEVIEQLTNSRTVYADDPNDVNSASLTLAELQALELDPAR
                     PSSYAGLSFDPRVPAGGVPSASLDLIYSLYNQIRWEYDADRGTYLRSQDRADGSGAMV
                     PSFDRLSDDRLQADNVLVFFAEHKYQNLQGTVLSIELAYVPKRYGLLFRDGRQFEIQW
                     SSRGLEFRVQDDQGHLVPLRPGVTFFEVVSYESSWDPAARIVRFHNPPLPTLTMTLTP
                     TPTATITPSATESAAPPPGTATP"
     gene            2846..>3088
                     /locus_tag="VI520_01900"
     CDS             2846..>3088
                     /locus_tag="VI520_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36667.1"
                     /translation="MPVLPRFDQFEGRHWETGSVSNALAYAGARVPQTKQRYSEALLL
                     GISGGAAFGYFVFDYKGHDPHVALLSRNTFDPLQTLL"
BASE COUNT          560 a          973 c          950 g          605 t
ORIGIN      
        1 ccccagctca ttgatcttgg cctccatcgc actgatgatt tcggcctgcg acttcccggc
       61 tgccttctcg gctacgtaca cgtcgattac ctgatcgccg tttggaccat acagggcctt
      121 ctggctcgcc acaccctgag attcgatatt gtcaaacatc gcaatggcct gccgctcggg
      181 cgtccgcgca gtactggtaa tggtgacgct gtcgagcttt gcttccttca tgatgtcttg
      241 caaaacctgg cgagagtgat cggagacggc cgcctgatcg gcatttgtgc caaagttgat
      301 cgacccggtc gtccctgcgc cagacgtgct tccccccgtt ccggaagcgg cagctgtact
      361 cccgccaagt ttgagcagcc gcgccgcctc gtcctcggct tgctgaatga gttggctcat
      421 cttcgacgcg ctctgagccg cgctatccaa cgcggcaacc aaccgcgccc agctcggcaa
      481 aatctccgag ttcatctcgc ggtagaacgc cttcgccccc tcccctatcc agtcgccccc
      541 ctcgagggcg tcctttgcct gatgcatctt gcgcaaactc tgaccacacg ccccggattg
      601 ctgcttgaac tgctgctgga cttgcctcaa catttcgtag tccgctcgaa ttcttggtgc
      661 agtcatctga tccactccta aacgaggaag tttaccgcgt tgggggccct ggtccctagt
      721 agtatttgtc gatcccacca aagccgaact gtttgttctg ctcggtccgc ttgatgaaat
      781 gcgccagccg cttcttgaat tcagccggtc gcttccgggc gccaccaatg taggccaccc
      841 gtatccgcaa ataactcggc gagaaggcct gcaatttttt ccagcttcga tttgtgattg
      901 cgctttaacc atgcgcgcca ttggctgcgg gtagccgcct gcagcagctt ggttttcggg
      961 atcggttgtt tcaccgccgc cctcagatcg ccacatccgg gacccagatg tatccgtccg
     1021 ggtcgcgcag gtagacctct cgcatgccgt gacccttgtc ggtcgtgggg accaggactt
     1081 catgaccgag ccgccgggct gcggcctccg cctggtccgg gtcacaacca tgcaatcgaa
     1141 tttcgacgcc gcccccgcgc ggagtaccgg gcgcgaccgt cccacccatc gcgtgcttgt
     1201 cgtaagtatg gtcagcgtgc agcatccact cggtcccgaa ccccgagcag accgcgaagt
     1261 cggggtcgga gtagacgacg ctggcgccca gcacttcgcg ttgaaatgcg agggcccgct
     1321 cgatctgcgc gacgagcaga ttgatggaca gggatttgag agtgtgggag taggcctttg
     1381 aggacatcca gggatcacca acacgcagct tcatgaatcc tccctttcga tacggcccac
     1441 cttatggggt ggcagtcccc ggcgggggag cggctgactc ggtcgcggat ggagtgatcg
     1501 tcgcagttgg cgtcggggtg agggtcatgg tcaaggtcgg cagtggcggg ttgtggaagc
     1561 ggacgatccg ggcagccgga tcccaggagg actcataact tacgacctcg aagaaagtga
     1621 ccccgggccg gaggggaacc agatgccctt gatcgtcctg cacccgaaac tcgagtcctc
     1681 ggctcgacca ctggatttcg aattgccggc cgtcccggaa cagcagcccg tatcgcttgg
     1741 gcacataggc cagctcgatg ctcaagactg tcccctgaag gttttgatac ttgtgctcgg
     1801 cgaagaagac cagcacgttg tcggcctgca gccggtcatc gctcaaccga tcgaacgagg
     1861 gaaccatggc gccgctgccg tcggctcgat cttgggagcg aaggtaggtg ccccgatcgg
     1921 cgtcgtactc ccaacggatc tggttgtaca ggctgtaaat gaggtcgagc gaagctgacg
     1981 gcacgccacc ggcggggacc cgtggatcga aggacaagcc ggcgtagctg gatgggcgtg
     2041 ccgggtcaag ttccagggcc tgcagctcag cgagagtcag cgaggcgctg ttgacatcgt
     2101 tcggatcgtc ggcgtacaca gtccgcgaat tggtcaattg ctcgatcact tccggcgagc
     2161 cgaagcggat gatcagcagc gcccctggat agaacagctt gatctcctga tagccgaccc
     2221 tccccgaacg gatcggcccg atggccgggt cactcgtcgt cagcttgggg tggctctcga
     2281 gcaggtcgga caagacctcc tggtagtctc cgtaatacac cgccaggaac cggctcatcc
     2341 cctccccgat ggacgtttcc cagacctgcg aggcggcgga gagtccggac tgtgggcggg
     2401 ccgagggcgg gaactcggta atcgagacca gcagcggaac cgtgagcaga gtttgggggt
     2461 cggaaaccgg gagcccggtc agcgggttga agccgggccg gtaggcaggg cccaccgcgg
     2521 gcaccgtcgc ggttgcgggg ggagcgggcg tcgcggtcga agcgctggcc gagcttctcg
     2581 gggctggggt aaccaccagg gtgactgaca gcgatccccc acgggcagcc gactcaggtg
     2641 tcgggttact agccgacggc tcggctggct gcggtcgttc aatcggccgc ctcacagcga
     2701 gcagggaaac gagaatcagc acagcggccg ccgcgccacc cagctgccgt cgcctggcag
     2761 aagtccagac tatgtctcga tcttgctcac cggacatgga atttctctat aatccccgca
     2821 gatattacct gaaaggagac cggacatgcc cgtgctcccc cgattcgacc aattcgaagg
     2881 acgccactgg gagaccggct cggtgagcaa tgccttagca tacgccgggg cccgggtgcc
     2941 gcagaccaaa cagcgctatt cggaggctct gctgctcggc atcagcggcg gggcggcctt
     3001 cgggtacttt gtgttcgatt acaagggaca cgacccgcat gtggcgctgc tctcccgcaa
     3061 cacctttgac ccgctgcaga ccctgctg
//
LOCUS       DATDPQ010000083         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_809634, whole genome shotgun sequence.
ACCESSION   DATDPQ010000083 DATDPQ010000000
VERSION     DATDPQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809634"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1993
                     /locus_tag="VI520_01905"
     CDS             <1..1993
                     /locus_tag="VI520_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF36668.1"
                     /translation="AWYRSVERSCKLIIVAGEAGVGKSRIGEEFLSGVTELGGKVLQA
                     RCYEGETTFAFRPWIDALRGGIGKGERLRRLAGLPRVRLAEAARLLPEIHDLIPDLPD
                     VGQAQGAAGHERFLDGLAWLAGTLAKGRHSGVVFLDDLQWADDPTLELLTYLLRHGPE
                     PPFAMLVGWRTDSAPSQQRLQEWLAVLPRGSYAITRLGRLSEAQVIELVDRTGLMGRP
                     TSGHRLYEETEGLPLFIAEYLRAIEGGASEPEWNLPASIRQMLAERLARIGSASRQLL
                     GAAAVIGRNFEYETLRSVSGRTPEETVAGLEELLATSLIREYPEVRPPLGPSYDFTHE
                     KLRVAALEGTTQARQRLLHSRVAKALRRGRPQGARAARIARHYELAGQEQEAAEFYVQ
                     AGDHARGLYANEQALAHYRSGLALGYPELGRLHEAIGDLLTLVGDYSGSVQNYEAALA
                     YAANSAIASLEHKLGSVYDRWGRWDRAESHFAAGLQTAGAQRAPILADWSLSLHRRGQ
                     RGQAQQLIQEALVEAERAGEPRALAQCHNILGIFARAQGDPGTARDQLLHSLEYAVSI
                     DDRSAQAAALNNLSLALADAEQLEQAIAAARQAIELSESIRDRHRSAALHSNLADLLH
                     RAGDEEQAKEHLRLSAGLFSEVGIEKDQFEPEIWKLVEW"
     gene            complement(2025..2900)
                     /locus_tag="VI520_01910"
     CDS             complement(2025..2900)
                     /locus_tag="VI520_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36669.1"
                     /translation="MQLDRKQLRDDIQRLYRAEHEALGESGTLDLLDKGRAWDLSETL
                     GAAGVVVFPHAGVADCGHQIAAAVHACLDCGTDRVIVVSVLHAFTDEMEAARIRVANG
                     EDPSREEFWGIQGPGITGRAEWLNDHALYSFRHFWKAETRRRGIRGPEVIERYPYLAG
                     GKPDRLPGIDELARLAEGAALVSTVDPFHHGIGYGNPPEESLHPQEGGLELARQVIGD
                     GMQLLAEGDYWGYNQHCVSAKSDGRDAGQVFRYLRGPMQGKILDLSFTDATDLYASPP
                     PTWVAGALFEWQLTR"
     gene            complement(2936..3009)
                     /locus_tag="VI520_01915"
     tRNA            complement(2936..3009)
                     /locus_tag="VI520_01915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2973..2975),aa:Val,seq:cac)
BASE COUNT          569 a          943 c         1027 g          523 t
ORIGIN      
        1 ggcctggtac cgttcggtcg agcgaagctg caagttgata atagtggccg gcgaggccgg
       61 agtggggaaa tcccgcatcg gcgaagaatt cttgagcggc gtcacggagc taggtggaaa
      121 ggttctgcag gcgagatgtt acgagggaga aaccactttt gctttccgcc cctggatcga
      181 tgcactgcgc ggagggatcg gaaaagggga gcggctgcga aggctggccg gcctgccacg
      241 cgtccggctg gctgaagccg cccggctgct gccggagatc cacgacctga tccctgatct
      301 tccggacgtc ggtcaggccc agggtgcggc gggccacgag cggtttctgg atggcttggc
      361 ttggctggcg ggtaccttgg cgaaggggcg ccattccggg gtcgtcttcc tggacgacct
      421 tcagtgggca gacgatccaa ccctcgaact cctgacctat ttgctgcgcc atggtcccga
      481 gcctccattc gcaatgctgg tgggctggcg aactgactcc gccccatccc aacagcggct
      541 gcaggagtgg ctggctgtgc ttcctcgcgg gtcctacgcg atcactcggc tcgggcgatt
      601 gagcgaggcc caggttatcg agctggttga ccgcactggc ttgatgggta ggccaaccag
      661 cggccaccga ctctacgagg agaccgaagg gctcccgttg ttcatcgcgg agtatctgag
      721 agcgatcgag ggcggggcta gcgagccgga atggaacctc ccggcttcga tccgccagat
      781 gctggccgag cgattggcca gaatcggcag cgccagtcgc caactcttgg gagccgcggc
      841 ggtcatcggc cggaatttcg agtatgaaac gctccggtcg gtgagtggtc ggactccgga
      901 ggagaccgtt gccggcctcg aagaactgtt ggcgaccagt ctgatccgtg aataccccga
      961 agtccgtccg ccgctgggtc catcctacga tttcacccat gagaagctgc gggtggctgc
     1021 gctcgaaggt acgacccagg ccaggcagcg cttgcttcac agccgggtgg ccaaggcact
     1081 caggcgagga agaccccagg gagcgagggc ggcccgcatc gcgcggcatt atgaacttgc
     1141 cggccaggag caggaagcgg ccgagttcta tgtgcaggcg ggtgaccatg cccgcggcct
     1201 ctacgccaat gagcaggcgc tcgcccacta ccgcagcggg ttggccctgg gatatcccga
     1261 gctgggccgg ctgcatgaag cgatcggaga tttgctgaca ttggtcggcg actacagcgg
     1321 atccgtccag aactatgagg ccgcgctcgc ttatgccgcg aacagcgcga tcgccagctt
     1381 ggagcacaag ctcgggagcg tatacgatcg ctgggggaga tgggatcgag cagagtctca
     1441 cttcgcggcc ggcctgcaga ccgccggagc tcagcgggct ccgattcttg ccgactggag
     1501 cttgagcctg caccgtcgcg gccagcgagg ccaggcgcag caattgatcc aagaggcatt
     1561 ggtcgaagcg gaacgagccg gcgagccgcg ggccctggcc caatgccaca acattctggg
     1621 gatcttcgcc cgggctcagg gtgatccggg aacggctcgg gatcaactgc ttcacagcct
     1681 ggaatacgcg gtctcgatcg acgatcggag cgcgcaggcc gcggcgctca acaatctatc
     1741 acttgccttg gccgacgccg agcaactcga acaggccatt gccgcggccc ggcaagcgat
     1801 cgaactcagt gaatcaattc gtgatcgtca tcgatcggcg gccctgcaca gcaaccttgc
     1861 cgatctgctg caccgggcgg gggacgagga gcaggccaag gaacatctgc ggctgtcggc
     1921 cgggctgttc agcgaggtgg ggatcgaaaa agatcagttt gagccggaga tctggaagct
     1981 ggtggagtgg taggtaggag gcgggaggaa gaaaaccggg tgagttagcg ggtcaactgc
     2041 cactcgaata gtgcgccggc cacccaggta ggtggagggg aggcatacaa atcggttgcg
     2101 tcggtgaagc tcaggtcgag gatcttcccc tgcatcggac cacgcagata gcggaacacc
     2161 tgacccgcgt cgcgcccatc actcttggcg gacacgcagt gctggttgta tccccagtag
     2221 tctccttccg ccaacaactg cattccatcc ccgatcacct gacgggccag ctcgaggcca
     2281 ccctcctggg ggtgcagtga ttcttcgggc gggttgccgt aaccgatccc atgatggaat
     2341 ggatcaacgg tcgacaccag cgcggcacct tccgccagcc gggcgagctc atcgatcccg
     2401 gggaggcggt cgggcttacc cccggccagg taaggatagc gctcgatcac ctcgggcccc
     2461 cgaatcccgc gccgtcgggt ctctgctttc cagaaatggc ggaagctata aagagcgtga
     2521 tcgttgagcc actcggcgcg gcccgtgatc ccggggcctt gaatgcccca gaattcctca
     2581 cggctgggat cctctccgtt ggcgactcga atccgggccg cttccatctc gtcggtaaag
     2641 gcatgcagca cactgaccac gatcacccgg tcggtgccgc agtcaaggca ggcatgcacc
     2701 gcagcggcga tctggtggcc gcagtcggcc accccggcgt gcggaaacac aacgaccccg
     2761 gcggccccca gcgtctccga gagatcccaa gcgcggccct tgtcaagcag gtccagggta
     2821 ccggattcgc ccagggcctc atgctcggcc cgatacaggc gctggatatc gtcgcggagt
     2881 tgctttcgat cgagctgcat cgtcggggag agcctcgaga gggcggtcct tcaggtgggc
     2941 gcggaggggc tcgaaccccc gacctcacgg atgtgaaccg tgcgctctaa ccagctgagc
     3001 tacgcgcccg agctgcttgc cattctagca tgcgccccgg aggctcacaa ggaatcgagc
     3061 tc
//
LOCUS       DATDPQ010000084         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_289014, whole genome shotgun sequence.
ACCESSION   DATDPQ010000084 DATDPQ010000000
VERSION     DATDPQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289014"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="grpE"
                     /locus_tag="VI520_01920"
     CDS             complement(<1..353)
                     /gene="grpE"
                     /locus_tag="VI520_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HLF36670.1"
                     /translation="MTKSGNNKPKPQDSESSGEEIESPPMEAPNEAELTENELTAEEL
                     DSVVAPQPIQSDLENIQAQADEYLDGWQRARAEFANYKRRTEREQAEARDRIAGNTLV
                     RFLGVMDDLERALKDR"
     gene            complement(374..1411)
                     /gene="hrcA"
                     /locus_tag="VI520_01925"
     CDS             complement(374..1411)
                     /gene="hrcA"
                     /locus_tag="VI520_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HLF36671.1"
                     /translation="MSVELLSERQETVLALVVHEYIEHARPVGSKQLVERYNLEVSSA
                     TIRNEMAALTESGYLRQPHTSAGRIPTEQGYRYFVGRLLGDTELPVSEKRLISHQFHQ
                     ARGDVDEWVRLAASVLAHHSRVASLVTAPHSERAALKLLELIGTQGRQVLMVLVLEGG
                     DVRQQMLTLSEVIGQDQLRQTAELITRQCARKDASGVRAQSSRFDTLGREIASLVAET
                     LQRADEFSAGEIHRDGLANVLAQPEFADADSGRNALRLLEEHSWLEEVLAKALSPGVG
                     GVQVVIGGEGAWEELKDYSMVLARYGAAGFATGTLGVLGPTRMAYGRSISAVRYVAGL
                     MSDLVVDAFAS"
     gene            1602..1937
                     /gene="trxA"
                     /locus_tag="VI520_01930"
     CDS             1602..1937
                     /gene="trxA"
                     /locus_tag="VI520_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLF36672.1"
                     /translation="MANIPELTEDAFKAEVLDAKVPVVVDFGAEWCHPCKQLDPVVEE
                     LAKEWAGKAKFLKLDADTNIDTTMRYQVFGLPTLILFKDGEPRERLTGYQPKHRIVHH
                     FGPYLDQEG"
     gene            2046..>2408
                     /locus_tag="VI520_01935"
     CDS             2046..>2408
                     /locus_tag="VI520_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLF36673.1"
                     /translation="MDRKRILTGDRPTGQLHLGHYVGSLQNRLRLQDSHECFFIIADL
                     HTLTTRPEKDYIRQVGEFVRELVLDYLAVGIDPERSVIFVQSGVPETFELNLILEMLI
                     SVPRLERLPSLKDMARAEN"
BASE COUNT          476 a          782 c          680 g          470 t
ORIGIN      
        1 cggtccttca aggcccgttc cagatcgtcc attaccccca aaaacctcac caaggtgttt
       61 ccggctatcc gatctcgggc ctcagcctgt tcgcgctcgg tccgcctctt gtaattagcg
      121 aactcggccc gggcccgctg ccaaccatct agatactcat cggcctgagc ctggatattc
      181 tcaaggtcgg actggatcgg ctggggggcg accaccgagt ccaactcttc agcggtcaat
      241 tcattctcgg tcagctcggc ctcgttcgga gcttccatcg gcggactctc gatttcctcc
      301 ccagagctct ccgaatcctg cggcttgggt ttgttgtttc cggatttcgt cacattgcct
      361 cgtcaggttc tcgctagctg gcgaatgcgt ccactaccag atcactcatc aatccggcca
      421 cgtagcgaac ggctgagatg ctgcgtccgt aagccatgcg ggtcggcccc aagaccccta
      481 gcgttccggt cgcgaacccg gccgccccgt agcgggcaag caccatggaa taatccttga
      541 gctcctccca ggcgccctcg ccgccaatga ccacctgaac gcctccgacc ccagggctaa
      601 gagccttggc caagacctct tcgagccagc tgtgctcttc caacaggcgc agggcgttcc
      661 ggccgctgtc ggcgtcggca aactccggtt gggccaatac gttggccaat ccatcccgat
      721 ggatttcgcc agccgagaac tcatcggccc gctgcaaggt ctccgccacc agggatgcga
      781 tctcccttcc caacgtatcg aaccgagagg actgagcgcg aacgcccgag gcgtctttgc
      841 gagcgcattg gcgggtgatt agctcggcgg tctggcgcag ctgatcctgg ccgatcacct
      901 cagacaaggt gagcatctgt tgccggacgt ctccaccttc caacacgagc accatgagca
      961 cctggcggcc ctgggtccca atcagctcaa gcagcttgag ggcggcccgc tccgaatggg
     1021 gagcggtcac caacgacgct acccgagaat ggtgggctaa caccgaggcc gccagccgga
     1081 cccactcgtc gacatccccc cgggcctggt ggaactggtg gctaatcaac cgtttctcgc
     1141 tgaccgggag ctcggtatcg cccaatagtc ggccgacgaa gtaccggtag ccctgctcgg
     1201 tcgggatccg gccagccgag gtgtgcggct ggcgaagata ccctgattcg gtcaaagcgg
     1261 ccatttcatt gcgaatcgtg gccgaactaa cctcgaggtt gtaccgctct acgagctgct
     1321 tcgagccgac cgggcgggcg tgctcgatgt actcgtgcac gacgagggcc aaaaccgtct
     1381 cctggcgttc actaagcagt tcgacgctca tgccataaac tcttagcact ctcgaagtga
     1441 gagtgctaac aacaacctgc ccatgatacc atgcggccgg aatcagcgtc aataggggtg
     1501 gtgggctaga gacatcgaag cgatcgaagt gagatagttg cttgacacga ctccctggct
     1561 ctactacact gcccggccga aaatcccaga ccgatcattc gatggcgaat atccctgagc
     1621 tgaccgagga cgctttcaag gcagaagtcc tggacgccaa agtgccggtt gtcgtcgact
     1681 tcggcgcgga atggtgccat ccttgcaagc agctcgatcc ggtcgtcgaa gaactcgcca
     1741 aggaatgggc tgggaaagcg aagtttttga aactggacgc ggataccaac atcgatacca
     1801 ccatgcgcta ccaggttttc ggcctaccga ccttgatcct cttcaaggac ggcgagccga
     1861 gagagcgcct caccggctac cagccgaagc accggatcgt ccaccatttt ggcccctacc
     1921 tagaccaaga aggttgaccc aggttccaat ccgccgaggg tcccgctcgg cggggccctg
     1981 atctcacaca ttcagattga cagggtccgg cctgccgcgt attcttgggc cggcggacac
     2041 cctcgatgga ccgaaaacgg attctcactg gagatcgccc gaccgggcaa ttgcacctgg
     2101 gccactacgt cggctcatta cagaatcggc tccggctgca ggacagccac gagtgcttct
     2161 tcatcatcgc cgacctgcac accctcacca ctcggcccga gaaggactac attcgccagg
     2221 tgggcgaatt cgttcgtgag ctcgtgctcg attacctggc cgtcggtatc gatccggagc
     2281 ggagcgtgat tttcgtgcag tccggggtcc ccgagacctt tgagttgaac ttgatcctgg
     2341 agatgttgat tagcgtcccg cggctggaac ggctgcccag cctcaaggac atggcgcggg
     2401 ccgaaaac
//
LOCUS       DATDPQ010000085         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1082052, whole genome shotgun sequence.
ACCESSION   DATDPQ010000085 DATDPQ010000000
VERSION     DATDPQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082052"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VI520_01940"
     CDS             <1..616
                     /locus_tag="VI520_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36674.1"
                     /translation="LALGILREQDRISAMELDRRLCEALPGLLTPDRRLVMAVLRSYA
                     ISESAGAIWSLRSEDRGEARQADRAEIQTLLARLGGRLGFEVEGTDPVRWADRAEGQQ
                     FGFWVDETARLGTAMRRQLEQSESGPSSGLEVVVIPGGRAALVAEKSRRDPLLQSWLE
                     SAPRVIKFRHVRRLAEDTTLERGNLLERLVLDPPGHEDPQLPLL"
     gene            complement(656..>2114)
                     /locus_tag="VI520_01945"
     CDS             complement(656..>2114)
                     /locus_tag="VI520_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLF36675.1"
                     /translation="DPSKPYKPRNYDERFHGPVTVRAALANSYNIPALKTLEFVGIYD
                     DPSTPQGEGLISFAQRLGITTFTRSDFGIALTLGGGDITLLELTGAYATFANGGLRLP
                     PVAITRITDHTGATVYEYTVPAGDRVIRPEHAFLITSILSDNTARTPAFGPNSPLLLP
                     FPAAAKTGTTDKPIRDNWTLGYTPDIAVGVWIGNADNSPIEQTSGVTGAAPIWNEFMQ
                     LAIAQLVGGDPTPFVRPASIIEVPICAVSGAEPSEWCPAHRVEFFASDQPPLPPELDL
                     WRKVWIDGYSQELASAVCPDFAEEKLGFAVPDPWGRKWIMESEEGKAWAERMGFEEEE
                     EIFFIPEATCDADSPRPLLVITAPLQGSILSSGPIPIIGRAAATGDFLDWILEVGVGS
                     DPDFWPDVAQSDVPVEQPAILFDWDPRDLPNGPVSLLLTVRSTRGGKASYRVHLTLSL
                     PTPTPTATPTPTFTPTATATPTPTPTATETPTATP"
BASE COUNT          376 a          631 c          734 g          373 t
ORIGIN      
        1 attggctctc ggcatcctgc gcgagcagga tcgcatttcc gctatggagc tcgaccggcg
       61 gctgtgcgaa gcgctgcctg ggctactcac cccggatcgc cgattggtga tggcggttct
      121 gagatcctac gctatctccg agagcgcagg cgcaatctgg agtttgcggt cggaggatcg
      181 aggcgaggcc cgccaggccg atcgggcaga gatccagacc ttgctcgcca ggctgggggg
      241 ccggttaggg tttgaggtgg agggtaccga cccggtccgc tgggcagatc gggcggaggg
      301 gcagcaattc ggtttttggg tggatgagac tgccaggctg ggcaccgcga tgcgccgcca
      361 gcttgagcag agcgagtccg ggcccagctc ggggctcgaa gttgtcgtaa tacccggagg
      421 ccgagccgct ctcgtggcgg agaaatcgcg gcgcgacccg ctgcttcaga gctggctgga
      481 gagtgcgccg cgagtcatca aatttcgtca cgtccgccgc ctggcggaag acacgaccct
      541 cgaacgcggc aatctactgg agcggctagt gctggatccg cccggtcacg aggatccgca
      601 gctcccgctg ctctagcctt tcaccaaccc atcgaattcg ctaatcaact gcgaattatg
      661 gcgtcgcggt cggcgtttcg gtggcggtcg gcgtgggggt cggggtcgcg gtggcagtcg
      721 gagtaaaggt cggggttgga gtggcggtgg gagtcggagt gggcaggctc agggtcagat
      781 ggactcgata gctggccttg cctccgcgcg tgctgcggac tgtgagcagc agtgagaccg
      841 ggccattcgg caaatcccgc gggtcccagt cgaaaaggat ggcaggctgc tcgaccggca
      901 cgtcgctctg ggcgacgtcc ggccaaaagt ccggatcgct gccgaccccg acttccaaga
      961 tccagtccag aaagtcaccc gtggcggccg cccggccgat gatcgggatc gggccggacg
     1021 acaggatcga accctgcagc ggagcggtga ttaccagcag cgggcgcggg ctgtccgcgt
     1081 cgcaggtggc ctccgggatg aagaagatct cttcctcctc ctcaaacccc atccgctcgg
     1141 cccaggcctt cccctcctcg gactccataa tccactttcg gccccatggg tctggcaccg
     1201 cgaatccgag cttctcttcg gcaaagtcag ggcatacggc cgaggccagc tcctgcgaat
     1261 agccgtcgat ccagaccttg cgccagagat caagctccgg aggaagcggc ggctgatccg
     1321 acgcaaagaa ctctacccgg tgggccgggc accactcgga gggttccgcc ccggaaaccg
     1381 cgcagatggg cacttcgatg atgctggcag gccggacgaa cggcgtaggg tcgcctccga
     1441 ccaattgggc aatcgccagc tgcatgaact cgttccaaat tggagcggcc ccggtcaccc
     1501 cggaagtctg ttcgatcggg ctgttatccg cattgccgat ccaaactcca actgcgatgt
     1561 cgggggtata gcccagggtc caattgtcgc ggatcggctt gtcggttgtc ccagtcttgg
     1621 ctgccgccgg aaagggaagc agcagcgggg agttcggacc gaaggccggg gtgcgcgcgg
     1681 tgttgtcgga gagaatcgag gtgatcagaa aggcatgctc cggccggatc acccgatcgc
     1741 ccgccgggac cgtgtactca tagacggttg cgccggtgtg atcggtgatc cgggtgatcg
     1801 ccaccggagg caggcggagg ccgccgttgg cgaacgtagc ataggctccg gtgagctcga
     1861 gcaaggtgat atcgccccca cccaaggtca gggcgatccc gaagtcgctg cgagtgaagg
     1921 tggtgatccc caagcgctgg gcaaatgaaa tcagcccctc tccctgcgga gtcgacggat
     1981 cgtcgtaaat gccaacaaac tcaagggtct tgagcgccgg gatgttgtag gagttggcga
     2041 gcgccgcccg gactgtgact gggccgtgga agcgttcgtc ataatttcgg ggcttgtacg
     2101 gcttggacgg gtcc
//
LOCUS       DATDPQ010000086         4296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1856760, whole genome shotgun sequence.
ACCESSION   DATDPQ010000086 DATDPQ010000000
VERSION     DATDPQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856760"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..428
                     /locus_tag="VI520_01950"
     CDS             69..428
                     /locus_tag="VI520_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF36676.1"
                     /translation="MLVKGIPMVLVFVTDLPRSVAWYQDTLELPLLYQAAGFASFQVG
                     QQRLALHTSEQAGQVNSNSGSIPVLQVEDYPAAKATLESRGCQFYFENTTPNATFGSF
                     RDPDGNPLQIMQNLKKE"
     gene            425..775
                     /locus_tag="VI520_01955"
     CDS             425..775
                     /locus_tag="VI520_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HLF36677.1"
                     /translation="MSELAGRELRPPRGGGVEPLKGAELQLLHEKLGAEWQVIDERHL
                     EKQYRFKNFQEALDFVNRVGELAESVDHHPDLCLGWGKATITIWTHSIGGLSEADFVF
                     AARADRLVGSPGSP"
     gene            complement(772..2133)
                     /locus_tag="VI520_01960"
     CDS             complement(772..2133)
                     /locus_tag="VI520_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HLF36678.1"
                     /translation="MKQVPKLTPEAAARMVPHGARLMVGGFGMTGNPVHLLHALADAG
                     TKNLTYISNNIGEPGIGGGLLLERGQIKKAIGSFFTSNPEAVAAAQTGRIELELLPQG
                     SFVEAIRAGGAGLGGFYTPTAVGTSLGQQAETKLIDGKVYAFVPALRAEVALIHAWKA
                     DTAGNLVYRMTEQNFNKSMATAADLVIAEVEQIVPLGDLDPDQIHTPGCYVDYLVQEH
                     TKPEDLGSSGSVAAREAGVAGARLRIAQRALLELRPGSVVNLGVGIPTLVAELIRPEH
                     SVTVHSENGMLGLGPSPEDGGALDYPVNAGKVPVTALPGSSYFDSADSFAMIRGGHVD
                     VAVMGGLQVDEQANLANWAAPGRPLLGVGGAMDLAEGAKRLIITMTHCDKDGTPKLVP
                     ACDLPLTAARAVDLLITELGVFEFSEGKPTLIEVMPGSSLEEIQAKTGAEFKTALRDR
                     PGG"
     gene            2210..3985
                     /locus_tag="VI520_01965"
     CDS             2210..3985
                     /locus_tag="VI520_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36679.1"
                     /translation="MEARTARDVLSQITSLRQHPKSELLFEIVTGGADLSSAGFPYIS
                     DTGFRIFRDSVPHLFRHGAAVLAWLATLEQELVEKPDLPLADLESIEQAASVVRTVVL
                     TASVTAPPDLWLLRHVLSTHKRLGILDWLVSGQVLDPEVFAREHGLNFKQLNTDLRFV
                     HSRGYLVKGAGDFAVASDPAAANVLKQFSDNPAEPQPNMLPRLKEWFSSPKQVDEQLR
                     AWLDLSVADQPTGSWVASRFQIELGYRLLPTVLGLRILEITKELGRGARLAVHIPNYT
                     AELGRLFELAGLAEEGIVNELGARVFERGPGAFGIIAAYHPYVNQLAELLRSHTIGAW
                     VHRGENVAASQDANRKTFEMANDKLDLFCRQYGYQYRVFIEHAAGRGEAIRQRYVRDG
                     EQEGKYFGADLEDAAIDQAIGLQEQGILPASLQFIRSADIGEPDRVIQFLAGKGLAGD
                     PTVMMVGNGFHEIRNQTNEKMVEVFRGYAHAGFVLIFTEESALDDEALLDTAWNSYHA
                     GFRYVHEMSGQGLRPPAEGEHGTQRWSWRKCGELAGYVFLDEFSYRSRTIYPYRRPMH
                     KNPSISVTYFCIPAKLAQQLGTKPD"
     gene            4082..>4296
                     /locus_tag="VI520_01970"
     CDS             4082..>4296
                     /locus_tag="VI520_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36680.1"
                     /translation="MRAIFLTTLAVLLAACGSSAVTGRELAPPSPAMDRLAASTSARS
                     EAELPQPYQEVGGVEPAISSASSSAESS"
BASE COUNT          813 a         1317 c         1346 g          820 t
ORIGIN      
        1 cggtatgata tggcggccga gatcatcgag acgatggccg aacgggtgcc cgaggcgagg
       61 ccggcctgat gctggtgaaa gggatcccca tggtgctggt gttcgtcact gacctgccgc
      121 gcagcgtggc ctggtatcaa gacacgctag agttgccgct gctgtatcaa gcggctggat
      181 ttgcctcgtt ccaggtcggc cagcagcggc tggccttgca cacgagtgag caggctggtc
      241 aggttaactc caatagcggc agcatcccgg tcttgcaagt cgaggactac cctgctgcca
      301 aggcgaccct ggagtcaagg ggttgccagt tctactttga gaacaccacc ccaaatgcga
      361 cctttggatc cttccgcgat ccggacggta acccgctcca gatcatgcag aacttgaaga
      421 aagaatgagc gaactcgccg gacgggagct ccggccgccg cgggggggcg gggtggagcc
      481 gctaaagggt gccgagctgc aattgctgca cgagaagctc ggggccgagt ggcaggtgat
      541 cgacgagcgg cacttggaga aacagtaccg attcaagaat ttccaagagg cgctcgattt
      601 tgtcaaccgg gtcggtgagc tggccgaatc ggtcgaccat caccccgacc tctgcctggg
      661 gtggggcaag gccaccatca ccatctggac tcacagcatc gggggcctct ccgaggcgga
      721 ctttgtgttc gccgcccggg cggatcggct ggtcgggagt cccggctcgc cctagcctcc
      781 gggtcgatcc cgcagggcag tcttgaattc agcgccggtc ttggcctgaa tctcttcgag
      841 cgacgacccc ggcatcactt cgatcaaggt cggttttccc tcgctgaatt cgaatacacc
      901 gagctcggtg atcaacagat cgaccgcccg cgcagcggtc agcggcaggt cgcaagccgg
      961 cacaagcttg ggggttccat ccttatcgca gtgagtcatg gtgatgatca ggcgcttggc
     1021 gccttccgcc aggtccatcg ccccaccgac cccaagcaac ggccgaccgg gggcggccca
     1081 attcgccagg ttggcctgtt cgtcgacttg caacccgccc atgacggcca catccacgtg
     1141 acctccccgg atcatggcga atgagtcggc gctgtcaaaa tagctgctcc ccggcagcgc
     1201 ggtaaccgga acctttccag cgttgaccgg ataatccagt gcgcccccat cttccgggga
     1261 ggggccgagc ccgagcatcc cattctccga gtgcacggtc acgctgtgtt ccggccggat
     1321 cagttcggca accagggtcg gaatcccaac ccccagattc acgacgcttc ccggccgcag
     1381 ctccagcaat gcccgctggg cgatccgtaa ccgcgccccg gcgacccccg cttcgcgggc
     1441 cgcgaccgaa cccgaggatc ccagatcttc cggcttcgtg tgctcctgca ccaggtagtc
     1501 aacatagcag cccggagtgt ggatctggtc ggggtccagg tcgccgagcg gaacgatctg
     1561 ttcgacttcg gcgatcacca ggtcggcggc ggtcgccata ctcttgttga agttctgttc
     1621 ggtcattcga tagaccaggt tgccagcggt atccgctttc caggcatgga tcaaggcaac
     1681 ctcggcccgc agcgcgggga cgaaggcata cactttgccg tcgatcagtt tggtttccgc
     1741 ctgctgccca agtgaagtcc cgaccgcggt cggggtgtag aagccgccca gccccgcccc
     1801 gccggcccga atggcctcga caaaagatcc ttggggcagc agctcgagct cgatccggcc
     1861 ggtctgggcg gctgctacgg cctccggatt ggaagtgaag aacgaaccga tcgctttctt
     1921 gatctgcccc cgctcaagca gcagtccgcc gccaatcccc ggttctccga tattgttgct
     1981 gatgtaagtc aggtttttgg tcccggcgtc ggccagcgcg tgcagcaggt ggaccggatt
     2041 tccggtcatt ccgaatccac ccaccatcag gcgggcgcca tgcgggacca tccgggcggc
     2101 cgcttcgggt gtgagtttgg gaacttgctt catgggggct cccggacgct cggcccttca
     2161 ggagtttgcg gttaccgggg actagatgat aatccagcag gccctagcca tggaagcacg
     2221 tacggcgagg gatgtgctct cgcagatcac cagccttcgc caacacccga aatccgagct
     2281 gctgtttgag attgtcactg gcggagccga cctgagctcg gccggcttcc cgtacatctc
     2341 cgacactggc ttccggatct tccgagacag cgtgccccac ttgtttcgcc acggggctgc
     2401 ggtgctggcc tggttggcga ccttggagca ggagctcgtt gagaaaccgg atctccctct
     2461 ggccgatctg gagtcgatcg agcaagctgc cagcgtcgtg cggacggtcg tgcttaccgc
     2521 cagtgtcacc gctccaccgg atctctggct gctgagacat gttctctcga ctcacaaacg
     2581 actgggcatc ctcgactggc tggtgtcggg gcaagtcctg gatccagaag tctttgcccg
     2641 cgagcacggg ctgaacttca aacagctcaa caccgatttg cgctttgtgc actcaagggg
     2701 ctatctggtg aagggagctg gggacttcgc ggtcgcctcg gatccagcgg ccgccaacgt
     2761 actgaagcag ttctcggaca accctgccga gcctcagccc aacatgctgc cgcggctcaa
     2821 agagtggttt tcatctccca aacaagtcga cgaacaactc cgcgcctggc tcgacctgag
     2881 cgtagccgac cagcccaccg gcagctgggt agccagccgg tttcaaattg agctcggata
     2941 ccggctcttg ccgaccgtgc ttggcctgcg gatcctggaa atcacgaaag agctgggacg
     3001 cggggcgcgg cttgcggtgc acattccgaa ctacaccgcc gagctcggcc gcctcttcga
     3061 gctggccggg ttggccgagg aggggatcgt aaatgaactg ggagcacggg tttttgagcg
     3121 cggtccaggt gcatttggga tcatcgctgc ctatcatccc tatgtcaatc agctggcgga
     3181 gcttctccga tcccatacga ttggggcctg ggtccatcgg ggcgagaatg tcgccgcgag
     3241 ccaggatgcg aaccgcaaga ccttcgagat ggccaatgat aagctcgatc tcttctgcag
     3301 gcagtatgga taccaatacc gcgtgttcat tgagcacgca gccgggcgcg gggaggcaat
     3361 ccgccagcga tatgtgaggg acggggagca ggaggggaag tactttggag ccgacctgga
     3421 agacgccgcg atcgatcaag cgatcgggtt gcaggagcag ggaatcctgc ctgccagctt
     3481 gcagttcatc cggtcggcgg atattggcga gccggatcga gtgatccagt tcctcgctgg
     3541 aaaggggctg gccggtgacc cgacggtgat gatggtaggg aatggttttc atgagatacg
     3601 caatcagacc aacgaaaaga tggtcgaggt cttccgtgga tatgcgcacg cgggctttgt
     3661 gttgatattc acggaagaat cggcactgga tgacgaggca ctgctcgaca ccgcttggaa
     3721 cagttaccac gctggcttcc gttacgtaca cgagatgtca gggcagggcc tgcgcccgcc
     3781 tgcggaaggc gagcacggaa cgcagaggtg gagctggcgc aaatgcggcg aacttgcagg
     3841 atatgtcttc ttggatgaat tctcataccg cagtagaacc atctatccct atcggaggcc
     3901 gatgcacaag aatccctcga tcagcgttac ttatttctgt attccagcga aactcgctca
     3961 gcaattggga acaaagccgg actaagggct gtccggcgcg gcgagcgccg ctccggaacc
     4021 tgttgggccc gaccggtgta tatttacgtg aagagaggag tactactccg agggggtatc
     4081 catgcgagcg atcttcttga cgaccctagc ggtcctgctg gccgcctgcg gctcatcggc
     4141 cgtcaccggg cgggaactgg ccccgccaag cccagctatg gacaggcttg ccgcctcgac
     4201 ctcggcccgc agtgaggccg agctcccaca gccctaccaa gaggtcggcg gagtcgagcc
     4261 agcgatttcc tcggcgagtt cctccgcaga gagctc
//
LOCUS       DATDPQ010000087         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2127926, whole genome shotgun sequence.
ACCESSION   DATDPQ010000087 DATDPQ010000000
VERSION     DATDPQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127926"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1006
                     /locus_tag="VI520_01975"
     CDS             80..1006
                     /locus_tag="VI520_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HLF36681.1"
                     /translation="MRRWEQYRTPEHLADALAILREPGGSAAIVAGGTDLLLDLQQER
                     HAAVTTLVDVSRVREMLQVSERDGWIEIGAAVPLNEIIRHPLVRANADCLIEASELIG
                     GPQVRNVATLGGNVAHALPAGDGTIALLALGSEALLAGTDGNRWQPLENLFEAPGKTT
                     FDRSREILVSFRFPAKRPDEASAFYRIMRPQGVAIAILNMAARIRFAADRTVLEVALA
                     LGPAGPRPLRARAAEAALLGRKLDESTLEDTVAALLSEVELRTSPHRATAEYRRHLLR
                     TLVRRTLLAAELRLRSAANANSNGAGPEPDPR"
     gene            1003..>2252
                     /locus_tag="VI520_01980"
     CDS             1003..>2252
                     /locus_tag="VI520_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLF36682.1"
                     /translation="MKPDRTPTTASELQLTVNGRLHRLSVGQSEMLSDVLRSRLSLTG
                     TKIACEEAECGTCTVLLDGAPVLSCVLPAFKAAGHSIVTIEGLATEGNLHPLQEAFVS
                     YGAVQCGFCIPGQIMTAAALLENNTDPSEGEIRQALKDTLCRCAGYPTIIRSVQAAAR
                     RLNTGEPIGVPDLPTSTQTRVVGQLVARPDAVDKVTGRARFADDYAFSEMLHGATLRA
                     GVPHGIVRRLDITRAQALNGVVAVLTAADIPGEHNHGLVIKDWPALVGLGERVRYVGD
                     AVALVAAETREIAQTAIGLIEVEFDLQPVVSDPVQARSADAPQLHANGNLLKHIKVRK
                     GDLEAGWVESEIVLEDTYYTPITEHAFLEPECSIARLTPDGRIEVVVGSQIPYADRSQ
                     IARCLGVADDQVRVIGALIGGGFGG"
BASE COUNT          386 a          749 c          737 g          380 t
ORIGIN      
        1 gcgcaggagg tacctgcgcc ggcgttgagc atccccgctt ttcgataagc gccggccggt
       61 ccccagcgcc tcgctaacca tgcgtcgttg ggagcagtac cggacccccg aacaccttgc
      121 cgacgcgctc gcaatcctgc gcgaaccggg tggcagcgcc gcgatcgtcg caggcggcac
      181 cgacctgctg ttggatctcc agcaggagcg tcatgcggcg gtcacgaccc tggtggatgt
      241 cagccgggtg cgtgagatgc tccaagtctc cgagcgtgac ggttggatcg agattggagc
      301 cgcggtcccg ctgaatgaga tcatccgcca tccgctggtg cgggccaacg ccgactgcct
      361 gatcgaggcc agcgagctga tcggaggccc tcaggtccgc aatgtcgcca ccctgggggg
      421 caatgttgcc catgctctgc cggcgggcga tgggacgatc gctttgctgg cgctcggctc
      481 cgaggcgctg ctcgctggca ctgacggaaa ccgctggcag ccgttagaga atctcttcga
      541 agcccccggc aagacgacct ttgaccgaag ccgcgagatc ctggtcagtt ttcgctttcc
      601 agccaagcgg ccggacgagg cttccgcctt ctatcggatc atgcggccgc agggcgttgc
      661 aatcgcaatc ctgaacatgg cggcccgaat ccgattcgcc gccgatcgca cggttctgga
      721 agtcgccctg gcgctggggc cggccgggcc gcggccgctg cgagccaggg ctgccgaggc
      781 agccttgctc ggccggaagc tggacgaatc gaccctcgaa gacaccgtgg cggcgctgct
      841 gtcggaggtg gaacttcgca ccagcccgca ccgggctacc gccgaatacc gccgtcatct
      901 acttcggacc ctggtgcggc gcaccctgct cgccgccgaa ctgcggctgc gctcggccgc
      961 caatgccaac tcgaatgggg ccgggccgga gccggatccc cgatgaagcc cgaccgaacc
     1021 ccgactaccg cgagcgaatt gcagctgacc gtcaacggcc ggctccatcg gctcagtgtc
     1081 gggcagagtg agatgctctc ggatgtcttg cgctctcggt tgagcctgac cggcaccaag
     1141 atcgcctgcg aggaagccga gtgcggaacc tgcacggtgc tgctggatgg ggccccggtg
     1201 ctctcgtgcg tccttccggc tttcaaggcc gccgggcact cgatcgtcac cattgaaggc
     1261 ctcgcgaccg aaggcaacct ccacccgctg caggaagcct tcgtctcata tggcgcggtg
     1321 cagtgcggct tctgcattcc tggccagatc atgaccgcgg ccgcgctgct ggagaacaat
     1381 actgatcctt cggaaggtga gatccggcag gcgctcaaag acacgctgtg ccgctgcgct
     1441 ggctatccca cgatcatccg ctcggttcag gcggcggcga gaagactgaa taccggcgag
     1501 ccgatcggag tcccggatct tccgacctcc acccagaccc gagtcgtcgg ccagcttgtc
     1561 gcacgcccag atgccgtaga caaagtgacc gggagggcac ggtttgccga cgactacgcc
     1621 ttctccgaga tgctgcacgg agcaaccctg cgggccgggg tccctcacgg gattgtgcgc
     1681 cggctcgaca tcacccgggc ccaggcattg aacggtgttg ttgcagttct cacggccgcg
     1741 gacatcccgg gagaacataa ccatgggctg gtcatcaaag actggccggc gttggtcggg
     1801 ctcggtgagc gggtgcgcta cgttggggac gcggtcgcgc tggtcgcggc agaaacccgc
     1861 gaaatcgccc aaacggcgat cgggctgatc gaggtcgagt tcgacctaca accggtagtc
     1921 tccgacccgg tccaggcccg ttcggcagac gccccccagc tgcatgccaa cggcaacctg
     1981 ctcaagcaca tcaaagtccg caaaggcgat ctggaagcgg gctgggtcga aagcgagatc
     2041 gtgctcgagg acacctacta cactccgatc accgagcacg cctttctcga gccggagtgc
     2101 agcatcgctc gcctcactcc tgatggtcgg atcgaggtgg ttgtcgggtc acaaatcccg
     2161 tatgccgacc gcagtcagat cgcccgctgt ctgggggtgg ccgacgacca ggtccgggtc
     2221 atcggtgcgc tgatcggcgg aggatttggc gg
//
LOCUS       DATDPQ010000088         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_290227, whole genome shotgun sequence.
ACCESSION   DATDPQ010000088 DATDPQ010000000
VERSION     DATDPQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290227"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..511
                     /gene="erpA"
                     /locus_tag="VI520_01985"
     CDS             89..511
                     /gene="erpA"
                     /locus_tag="VI520_01985"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HLF36683.1"
                     /translation="MATIDLPLTETLVETREDITLTENAVSRVRKLIEERQLTGHSLR
                     VFVSGGGCSGMQYGMGLEPEPRENDLRFSFDDVQVVVDPMSISYLAGTVIDYTDELMG
                     GGFRIENPNAVSACGCGHSFRTAESSDAHSHSHSDGCC"
     gene            576..959
                     /locus_tag="VI520_01990"
     CDS             576..959
                     /locus_tag="VI520_01990"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HLF36684.1"
                     /translation="MQQFPRLFTLEQANQLLQTLRPLVDHLVATRDQIVTIRPELDSG
                     VQKALGNGASKATGELLKLFRRIERLIKEVHSYGVLVKDVDQGLLDFPAEKDGRVVFL
                     CWKHGEASIGHWHDLDAGFSGRKLL"
     gene            complement(956..1600)
                     /locus_tag="VI520_01995"
     CDS             complement(956..1600)
                     /locus_tag="VI520_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLF36685.1"
                     /translation="MANRRNAPARQVAWRILSAAGVLAVALAVPAAVTTARAAEFSQP
                     PTVSIPTVTGTPTGPMIVVPDQVNVRAGPCTSCELIGVLIAGQEAPAIGRSPGGDWIQ
                     IAYPGVPGNVGWVYSPLVVLDPPGASLPIVEPPPTATPRASATIDPTLAAQFNLGDVT
                     PTRLPTFTPAGPVAQPTFAAEAPESRSVIPPIVAIAGLVMVGTFGMLISILRGG"
     gene            complement(1623..>2954)
                     /locus_tag="VI520_02000"
     CDS             complement(1623..>2954)
                     /locus_tag="VI520_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLF36686.1"
                     /translation="SALERLLQTYSTPVELFYEGERIVLSPSQVGFQVDTEAMLAAGE
                     LARTNTDFWSGFWDFLWDRPGEPQRVPLRAEYSRPQLEIALRDVAARYDEPAVPSQPI
                     PGSADFQAGSPGRVLDVERSAELVGEVLSAPGDRRVNLPILESQANRPSLPTLETLLK
                     QNLDVEAFDGLVVIYVMDLRTGQELHFGYYLNEDISVSPDIAFDAGSTIKVGIATAFF
                     RYFDEPLDDEASRWLVEMLTQSGNDPANWLMERIDFGQGPVAVTESLSALGFENSYIV
                     AWFENPRRFTTPAQLIAASPPATPANQRLDINTRPNQLNQTTASELGMLLADLYSCAK
                     GGGGLLAAFPTELRPEECARILDLLSQNRIAWLIEASVPDGTRVAHKHGWTQSPLDFI
                     SDAGIVYSPGGDYVVSIFLWDDREMVWEPTAALFSQLGRAVYNYFNPPASG"
BASE COUNT          584 a          877 c          948 g          545 t
ORIGIN      
        1 tgccaggaga attgacaatg gcggcgaagg ttgataaact ctaggttgat cataagaatt
       61 ccgagaaacg cggaacaggg accgcccaat ggccacaatt gacctaccgc ttaccgagac
      121 gctagtcgaa acgagagaag atatcaccct gacggagaat gccgtcagcc gggtacggaa
      181 gttgattgag gaacgccagc tgaccggcca ctcgcttcga gttttcgtgt cgggcggcgg
      241 ctgctctggc atgcagtacg gcatggggct ggagcccgag ccgcgggaaa acgatctacg
      301 cttctccttt gacgacgtcc aggttgtggt ggatccgatg tcgatcagct acctggcagg
      361 cacggtgatt gactatacgg acgagctgat gggtggcggc tttcggatcg agaatccgaa
      421 tgcggtttcc gcctgcggtt gtgggcattc cttccggacc gccgaatcat ccgacgccca
      481 ctcgcactcg cactctgatg gctgctgcta gcagcccgat cgcttagcag atcgcctttt
      541 acaggtcgct aaatgcgacc tgtacttttt tcagcatgca gcagttccct cggttgttta
      601 ccctcgagca agccaaccag ctattgcaga ccctgcggcc actggtggac cacctggtcg
      661 ctacccgaga tcagattgtg accatccggc ccgaattgga ttcgggggtt cagaaagcgc
      721 tggggaacgg cgcgagcaaa gcgactgggg agctgctaaa actgtttcgg cgaatcgaac
      781 gtctcatcaa ggaagtgcac tcctatgggg tgctggtgaa agacgtcgat caagggttgc
      841 tcgatttccc agccgagaag gacggccggg tggtattcct ctgctggaaa cacggcgagg
      901 cctccatcgg ccactggcac gacctcgacg ccggcttcag cgggcgcaaa ctgctctagc
      961 cgcctcgcaa gatcgaaatc agcatcccga aggttccaac catgaccaac ccggcgatcg
     1021 cgacgattgg cgggatcacg ctgcgcgact cgggtgcctc ggccgcaaag gtcggttggg
     1081 cgaccggacc ggcaggcgtg aaggtcggca agcgggtggg ggtcacatct cccaggttga
     1141 attgggcggc cagggtcgga tcgatggtcg cgctcgcccg tggggtggca gtgggtggag
     1201 gctcgacgat cggtagcgag gctcccgggg gatcgagcac gacaagaggc gaatagaccc
     1261 aacccacgtt gcccggaacc cccggatagg caatctggat ccagtctccg cccggggagc
     1321 gcccgatggc gggggcttcc tggccggcga ttagcactcc gatcagttca catgaggtgc
     1381 acgggccagc acgcacattg acttgatcag gaaccacaat catcggaccg gtgggggtgc
     1441 cggtcacggt cgggatcgag accgtgggcg gctggctgaa ctccgccgcg cgggcagtcg
     1501 tcaccgcggc tggaaccgcc agcgccacgg ctagcacgcc tgctgctgag aggatccgcc
     1561 aggcaacctg ccgtgcgggt gcgtttcgtc ggttggccat gcttggttgg ggatcgcccc
     1621 ggctagccgg aagccggagg attgaagtag ttgtagaccg cccggccgag ctgggagaaa
     1681 agggcggcgg tcggctccca gaccatttca cggtcgtccc agaggaaaat ggacaccacg
     1741 tagtcgcctc ccggcgagta gacgatgcca gcgtcgctga tgaaatccag cggcgattgg
     1801 gtccagccat gtttgtgcgc gactcgggtc ccatcgggga cgctggcctc gatcaaccag
     1861 gcgatccgat tctgtgagag caggtccagt atccgggcac actcctccgg tcggagctcc
     1921 gttggaaacg ccgccagtaa gcctccccca cccttggcac agctgtacag gtcagccagc
     1981 agcattccca attccgaagc cgtggtctgg ttgagctggt tgggacgggt gttgatatcg
     2041 agcctttgat tggcgggggt cgccggcggt gaggcggcaa tcaactgggc gggagtggtg
     2101 aaccggcgcg ggttttcaaa ccaggccacg atataggagt tctcgaatcc caacgccgaa
     2161 aggctctcag tgacagccac cgggccctgt ccaaagtcaa tccgttccat cagccagttc
     2221 gccggatcgt tccccgattg ggtgagcatt tccaccaacc agcgactggc ttcgtcatcc
     2281 agcggttcat cgaagtagcg gaagaacgcg gtggcgatcc cgactttaat cgtcgagccg
     2341 gcgtcgaatg cgatgtcggg agaaaccgat atgtcttcgt tgaggtaata gccgaaatgc
     2401 agctcctggc cggtccgcaa gtccatcacg tagattacca ccagcccatc gaaggcctcg
     2461 acgtccaggt tctgcttcag cagggtctcc agagtgggca gtgacggccg gtttgcctgg
     2521 ctctcgagaa tcggaagatt tacccgccga tcgccgggcg cgctgaggac ctcaccgact
     2581 agctccgccg accgctcgac atcgagcacc cggccgggac ttccggcctg gaagtcggcg
     2641 ctgcctggaa tgggttggct gggcactgcc ggttcatcgt aacgggcggc gacgtctcgc
     2701 agggcaattt ctagctgcgg ccgagagtac tcggcccgca gcggtaccct ctgcggctca
     2761 cccggtcggt cccagaggaa atcccaaaac ccgctccaga aatcggtgtt ggtcctggca
     2821 agttcgccag cggccagcat ggcctccgtg tcgacctgga agccgacctg ggacggggat
     2881 aacacgatcc gctcaccctc gtagaacaat tcaaccggag tgctgtaggt ttgcagcaat
     2941 cgctccagcg ccga
//
LOCUS       DATDPQ010000089         6390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1339533, whole genome shotgun sequence.
ACCESSION   DATDPQ010000089 DATDPQ010000000
VERSION     DATDPQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339533"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /gene="gltX"
                     /locus_tag="VI520_02005"
     CDS             <1..1383
                     /gene="gltX"
                     /locus_tag="VI520_02005"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLF36687.1"
                     /translation="ARQTGGQFIIRIEDTDRKRFVPGSEEEVLESLDWLGLHWDEGPD
                     RGGTYGPYRQSERVELYQQHAEQLVEGGLAYFCFCSPQRLSTLRQEQERRKQPPHYDR
                     LCRRLAVSEAAARVQAGESHVVRLKVPESGRTIAVDRLRGEIQVENSSLDDAILLKSD
                     GLPVYHLAAMVDDRLMGITHVLRSSEWLPTLPLHVLIYQAFGWEQPVWVHLSVFLSPS
                     GKGKMSKRDSVSGASSIYPLDLREQGYLPEAVLNWLVLMGWSYDDRTEVFDLAGLIQR
                     FSLDRLNPSPAAVNYSKLDHFNGIHIRSLPPGELTRRLVTFFVRHGLEVTETDLISLV
                     PLIQERIRTLDEAVEIAGFMFRDTVSPAAADLIGQGMTADASQGALRRAQQVILDRDR
                     LEVESLEAELRGLAAELGLSVGQLFTVLRIAITGQRVSPPLIETMVLLGKEQVFERLG
                     RAVELLAGVG"
     gene            1440..1510
                     /locus_tag="VI520_02010"
     tRNA            1440..1510
                     /locus_tag="VI520_02010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1472..1474,aa:Gln,seq:ctg)
     gene            1536..2628
                     /gene="prfB"
                     /locus_tag="VI520_02015"
     CDS             join(1536..1601,1603..2628)
                     /gene="prfB"
                     /locus_tag="VI520_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLF36688.1"
                     /translation="MQDLIDALETSRARIQELLVRLDFPKLEKELSELERAAADPQLW
                     QDSDGAQVKMKRLGRLRQEVESWRALRQRILDAIELAELGDESLRADIGSELEAVDSE
                     LDGRELATSLSGKYDADDAILSVHAGAGGTDSQDWAQMLERMYLRWCERRGFGAEILD
                     RSPGEEAGIKSVTIAVDGEYAYGYLRAERGVHRLVRISPFDSGNRRHTSFALAEVYPQ
                     VETETEIKIDPKDIKVDTFLSAGAGGQNVQKNETAIRIKHLPTGVVVTSQNERSQTQN
                     RENAMRVLRAKLLDLERQKREEQLAAIKGQHVSAEWGSQIRSYVLHPYQLVKDHRTGL
                     EIGNTAAVLDGDLDGLINAYLKTQTIAPG"
     gene            2697..2924
                     /gene="secG"
                     /locus_tag="VI520_02020"
     CDS             2697..2924
                     /gene="secG"
                     /locus_tag="VI520_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLF36689.1"
                     /translation="MSTYLNLALVLVSIALIVLVLLQSKGAGLGGLGGGDFGGSGYHV
                     RRGVERLVFNLTIVLSVLFFLLALLNVILGG"
     gene            2940..4637
                     /locus_tag="VI520_02025"
     CDS             2940..4637
                     /locus_tag="VI520_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HLF36690.1"
                     /translation="MRRIGWQLAIAVGGLLLVLGLLLGQTPGPEADLLQPVQGGSYAE
                     ALIGRSIRLNPLLDRYNQADRDIDRLIYGSLIRFNSRGEPVPELAENFAISADATLYN
                     FTLREDVRWHDGEPVTADDVIFTFSKFKEGGLPGDEDLRSLWQEVEIVKLDDKNVQFQ
                     LSESYAPFLDYLTVGLLPDHLLRGVSAAELAGHPFNREPVGSGPFQFESFLLAEDGQV
                     SGVSLTAFEQYAPGRPFLERIEFRFFADSQAALQAFRDGTVDGIGNVTNEILEAVLAE
                     PGLNLYSARAPQTATVFLNYRSTEKPFLAQKEFRQALLLAVNREQLIGSLLAGQGLPA
                     ASPILPGNWAHIDGLNPLPYDPELAAEILSDLGWELPAGVVRGAPEYVRANGEQELSF
                     ELAYPQVAPYEELASMLQGAWAAVGIRTELTPVPPEELLPERLEPRRFEAVLAEIDTA
                     QLGDPDPYPFWHDSQVETGQNYSGYQDRNISIWLEQARITPDVRRRQTLYRDFQYRFR
                     DQVPALLLFHPIYNYAVSAEVRGATLGPIQDPSDRFQQIAQWYVLIRRGFTTTDGAG"
     gene            4680..5393
                     /locus_tag="VI520_02030"
     CDS             4680..5393
                     /locus_tag="VI520_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLF36691.1"
                     /translation="MSEPKLENIQPGERVSGTVTSVELAGAFVDIGAGVPGFLHISRL
                     KPGKVNRVEDVLVPGQTVELWVSRVDPVAQRLELSMIRPVTLKWGQIQPGMQLRGEVI
                     RIEPFGAFVEVGAERPGLIHVSEISDEYVRDPRDQLRVGEELAVTVLEVDQKKKQIRL
                     SLKAVQPAEELEPEEVPQLPTAMELALRQALEASPPNRSTGKPKVKPDQHRNELEDIL
                     ARTLSSRLRTSSGESEEGK"
     gene            complement(5442..5690)
                     /locus_tag="VI520_02035"
     CDS             complement(5442..5690)
                     /locus_tag="VI520_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36692.1"
                     /translation="MPRIRCHYIDCVFLEGGYCGASTVEIDPDEGCMTYTHVAEVGAN
                     EELNDEELEEIWEEDDEELYKADEDEEEDDTWAEEEEA"
     gene            complement(5718..>6390)
                     /locus_tag="VI520_02040"
     CDS             complement(5718..>6390)
                     /locus_tag="VI520_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLF36693.1"
                     /translation="THGRLLVERELRRLGIDGIQIDKLAQDLNYPGGDDLYAAVGVGD
                     INLGKIVKRVVERDEAKLGFAPQVERRPAAVHSAEEVQVLGLSGLLTKLGLCCNPMPG
                     DPIIGYVTRGRGATIHRKDCPNVLRMKDKERLVSVSWGQAKRTIPVSVRIRAYDRDGL
                     MRDVSTLVANEGINMTSIHVSTTESLAIFDLVMGITDLPQLSRILNRLEALPNVLEAR
                     RVQPG"
BASE COUNT         1267 a         1952 c         1934 g         1237 t
ORIGIN      
        1 gctcgtcaaa ccggcgggca attcatcatt cggattgaag ataccgatcg gaaacggttc
       61 gttcccggtt ccgaggaaga agtcctcgag tcgctcgact ggctcggact gcactgggac
      121 gaaggtcccg accgcggggg aacctacggt ccctatcggc aatctgagcg agtcgaactg
      181 tatcagcagc atgcagagca gttggttgag ggcggcttgg cctatttctg tttctgctcc
      241 ccccaacgat tgagtaccct gcgccaagaa caggagcgtc gaaaacaacc accgcactac
      301 gaccggctct gccgtcggct ggcggtctcg gaggctgcgg cccgagtcca ggccggcgaa
      361 tcgcacgtgg ttcgattgaa agttccggaa agcgggcgga ccattgcagt cgaccggctg
      421 cggggcgaga tccaggtcga gaactcgagc ctggacgacg ccatactgct caagtccgat
      481 ggacttccgg tttaccacct ggctgccatg gtggatgatc gattgatggg catcacccac
      541 gtgctgcgca gttcggagtg gctgccgacg ctaccgctcc acgtgctcat ctaccaggcg
      601 ttcggctggg agcagccggt ctgggtgcac ctctctgtat tcctcagccc atctggcaaa
      661 ggaaagatga gcaaacggga ttccgtgagt ggagcaagct caatttaccc gctggacctg
      721 cgtgagcagg gctacctccc ggaagccgtg ctgaattggc tggtcctcat gggatggtca
      781 tacgacgatc ggactgaggt cttcgacctg gcgggcctga ttcagcggtt cagtcttgat
      841 cggctaaacc caagcccggc agccgtgaac tacagcaagc tcgaccactt caacgggatc
      901 cacattcgca gcctcccgcc cggcgagctg acccgcaggc tggttacctt ctttgtgcga
      961 cacgggctgg aagtcaccga gaccgacttg atctcgctag ttcccttgat ccaggagcga
     1021 attcggactt tggatgaggc ggtggagatc gccggattca tgttccgcga cacagtctcc
     1081 cctgctgcgg cggatctaat cgggcagggc atgactgcag acgcctccca gggcgcactg
     1141 cgacgagccc agcaggtcat cctggatcgg gatcggctgg aggtcgaaag cctggaagct
     1201 gagctgcgcg ggcttgcagc agaactcgga ttgagtgtag gtcagctatt cacggtattg
     1261 agaatcgcaa tcaccggaca acgggtcagc ccgccgctga tcgaaacaat ggtgctgctt
     1321 ggaaaggagc aggtgttcga acggctcggg cgagccgttg agctgctggc gggcgtcggc
     1381 tgaggttccc actcgccgtg agggccaacg caacccgcat gatacaatcg ccgccgcact
     1441 gggggttcgt ctagcggtag gacaccgggc tctgaacccg tgaaccgagg ttcgaatcct
     1501 cgacccccag cctcgggcag agtgccaaag ctgaaatgca agatctgatc gacgcgcttg
     1561 agaccagccg ggcccgcatc caggaactcc tggtgcgtct ttgatttccc aaaactagaa
     1621 aaggaactca gcgagttgga acgggcagcc gccgatcccc aactctggca ggattcggac
     1681 ggcgctcagg tcaagatgaa acggcttggc cggctgcgtc aggaggtcga gagctggcgt
     1741 gccctgcggc agcggattct ggatgccatc gaacttgccg agctcggtga cgaatcgctg
     1801 cgggcggata tcgggtcgga gctcgaggcg gtcgatagcg agttggacgg ccgggagctg
     1861 gcgacctcgc tttcaggcaa gtacgacgcc gacgacgcaa tcctttcggt ccacgccggc
     1921 gcggggggaa ctgattctca ggactgggcg cagatgttgg agcgaatgta cttgcgctgg
     1981 tgcgagcgcc gcggtttcgg cgccgagatc ctcgatcgta gccctggcga agaggcgggg
     2041 atcaagagtg tgactattgc ggtagatgga gagtatgcct acggctatct gcgggccgaa
     2101 cgaggtgtcc atcgactggt ccggatttcg ccgtttgatt ccggcaatcg tcgccacacc
     2161 tcctttgcac tggcagaggt ctatcctcag gtcgaaacgg agaccgaaat caagatcgat
     2221 ccgaaggaca tcaaagtcga cacttttctg tcggcaggcg ctggcgggca gaacgtccag
     2281 aaaaacgaga ccgcgattcg catcaagcac ctgcccaccg gagtcgtggt gaccagtcag
     2341 aacgaacgat cacagaccca aaacagggag aacgccatgc gggtgctgcg ggcgaagttg
     2401 ctcgacctgg aacgccagaa gcgcgaagaa cagctggcgg cgatcaaggg ccagcacgtc
     2461 agtgccgaat ggggaagcca aatccgctcc tacgtgcttc acccctatca gctggtcaag
     2521 gaccatcgga ccggcctcga aatcggcaac acggcagccg tcctggatgg agaccttgac
     2581 gggctgatca acgcctattt gaagacccaa accatcgctc caggctgaca gtccccctgc
     2641 tgaccagcct gcaccctctc actatgctat aatccgccgg cggtaagtgc ggctccatgt
     2701 cgacgtatct aaacctcgcg ctagtcctcg tttcaatcgc actgatcgtg ctggtgctgc
     2761 tgcagagcaa gggcgccggg cttgggggcc tcgggggcgg cgatttcggc ggcagcggat
     2821 atcacgtgcg gcgtggggtt gagcggctcg tgttcaacct taccatcgtt ctcagcgtcc
     2881 tcttcttcct gctagctctc ctcaacgtca ttctaggcgg ctaggccccg ctcgtcatca
     2941 tgcgcaggat cggctggcag cttgcgatcg ccgtcggcgg cttgctgctc gtgctgggac
     3001 tgctgctcgg acaaaccccc ggtccggagg ccgacttgct acagccggtc caaggaggct
     3061 cctacgcgga ggccttgatc ggccggtcca tccgcttgaa ccccctgctg gatcgctata
     3121 accaggccga tcgcgacatt gaccgattga tttacgggag cctgatccgc ttcaactccc
     3181 gaggcgaacc ggtcccggaa ctggccgaga actttgcgat ctctgccgat gcgaccctct
     3241 ataacttcac gctgcgggag gatgtccgct ggcacgacgg cgagccggtc acggcggatg
     3301 atgtgatctt caccttctcc aagttcaagg aaggtgggct acccggagac gaagatctac
     3361 gatctctttg gcaggaagtc gagatcgtca agctggacga caaaaacgtc caatttcaac
     3421 tctcagagag ctacgcccct tttcttgact atctgacggt cggattgctg cctgatcacc
     3481 tgctgagagg cgtctcggcg gctgagctgg ccggccaccc attcaaccgc gagccggtgg
     3541 ggagcggacc ctttcagttc gagagtttcc tgctggccga ggatggtcag gtcagcgggg
     3601 taagcctgac cgcgttcgag cagtacgctc ccggtcggcc tttccttgag cggattgagt
     3661 tccggttttt cgccgactcc caggcagccc tccaggcatt tcgggacgga accgtagatg
     3721 gcatcggcaa cgtgaccaac gagattctcg aggcggtcct tgcagagccc gggctcaacc
     3781 tatactcggc tcgcgccccg cagaccgcga ccgtcttcct gaattatcgc agcaccgaaa
     3841 agcccttcct ggcgcagaag gagttccgcc aagcgctcct gttggcggtc aatcgagaac
     3901 aactcattgg ttccctactg gccgggcaag ggctgccggc cgccagtccg atcctgccgg
     3961 gcaattgggc ccacattgat ggattgaatc cgctccccta tgatccggag ttggccgccg
     4021 agattctgag cgacctgggt tgggagctgc cagccggggt cgtccgcggc gcgccggagt
     4081 acgtccgcgc aaacggcgaa caagagcttt ccttcgagct ggcttatccc caggttgcac
     4141 cttatgaaga gctggcctcc atgctgcaag gggcatgggc cgcggttgga atccggaccg
     4201 agttgacccc agttccgccc gaggagttgc tacccgagcg actggagccg cggcgcttcg
     4261 aagccgtgct ggccgaaatc gataccgccc agttgggcga tccggatccc taccccttct
     4321 ggcacgactc gcaggtcgag actggccaaa actactccgg ctatcaggat cgcaatatca
     4381 gcatttggct cgagcaggcc cgcattaccc ccgacgtccg gcgacgccag acactgtatc
     4441 gagatttcca gtaccggttt cgggatcagg tcccggcgct gctactcttc cacccgatct
     4501 acaactatgc ggtcagcgcg gaagtgcgcg gcgccaccct cggcccgatt caagacccca
     4561 gtgatcggtt tcagcagatc gcgcagtggt acgtgctaat ccggagagga ttcaccacaa
     4621 ccgacggggc tggttgagga ccggcccgat gatataatcc ccggcttcaa tcccgaggca
     4681 tgagcgaacc caagctcgag aacatccaac ccggtgagcg ggttagcggc accgtaacca
     4741 gtgtagagct ggccggtgcg ttcgtggaca tcggcgccgg agtcccaggg ttcctccaca
     4801 tttcgcggct aaagccaggc aaggtcaacc gggtcgagga cgtgttggtg cccggccaga
     4861 ccgttgaact atgggtcagc cgagtggatc cggtggctca gcgcctcgaa ctcagcatga
     4921 tccggccagt aacgctgaag tggggccaga tccaacctgg aatgcaattg cggggagagg
     4981 tcattcggat tgaacccttc ggggcatttg tggaagtcgg ggccgagcga cccggactga
     5041 tccacgtaag cgaaatcagc gacgagtatg ttcgcgatcc acgggaccaa ctccgggtcg
     5101 gtgaagagct ggcggtgacg gtgctagagg tcgaccagaa gaagaaacag atccggctca
     5161 gcctgaaggc cgtccagccg gcagaggagc tcgagccgga ggaggtgcca cagctgccca
     5221 ccgcgatgga actggcattg cgccaggccc tggaggcgtc tccacccaac cggagtactg
     5281 ggaaacccaa agtcaagcct gatcagcatc gcaatgaatt ggaggatatc ctcgcccgaa
     5341 cactgtcttc cagattgcga acctcgagcg gggaatctga ggagggtaaa taatggcgcc
     5401 cggatgggcg ccgccaaacc ttcgagagtg acgattagcg gctaggcctc ttcctcttcg
     5461 gcccacgtgt catcttcctc ttcatcctcg tcggccttgt acagctcctc atcgtcttct
     5521 tcccagattt cttccaactc ctcgtcgttc agctcctcgt ttgccccaac ttcagcgaca
     5581 tgggtgtagg tcatgcatcc ctcgtccggg tcgatttcga ccgttgaggc gccgcagtag
     5641 ccgccttcca ggaacacaca gtcaatgtaa tggcagcgga tcctaggcaa cggttctcct
     5701 tggcacggtc tcggtcatca tcccggctgg acacggcgtg cttccagcac gttcggtagg
     5761 gcctccaacc gattcagaat ccgactcagc tggggcaggt ccgtgatccc catgaccaaa
     5821 tcaaagatag caagactctc tgtggtcgag acatggatcg aggtcatatt aataccctca
     5881 tttgccacca gcgtcgatac atcccgcatc aagccgtcgc gatcgtaggc ccggattcgg
     5941 accgacaccg gaatggtccg cttagcctga ccccacgaca cgctgaccaa ccgctccttg
     6001 tctttcatcc ggagcacatt ggggcaatct ttccggtgaa tggttgctcc ccgcccgcgg
     6061 gtgacgtatc cgataattgg atcgcctggc atcgggttgc agcatagccc aagtttggtc
     6121 agcagaccgc tcagccccaa gacctgcacc tcctctgcgc tgtgcactgc cgccggccgg
     6181 cgctccacct gaggggcgaa cccgagtttg gcctcgtctc gctccaccac tcgtttgacg
     6241 atttttccca ggttgatgtc gcccaccccg accgcggcgt aaagatcatc gcctccgggg
     6301 taattcaaat cctgggcgag cttgtcgatt tggatcccgt caattccgag ccgccgcagc
     6361 tcacgctcga ccagcagccg gccatgggtg
//
LOCUS       DATDPQ010000090         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2643597, whole genome shotgun sequence.
ACCESSION   DATDPQ010000090 DATDPQ010000000
VERSION     DATDPQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643597"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..767
                     /locus_tag="VI520_02045"
     CDS             240..767
                     /locus_tag="VI520_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLF36694.1"
                     /translation="MEVTEAIRLKRAVRSFAPEALPEQGVRAILNAGRRAQSAKNRQA
                     WHFIVIRDRETLGELAKLGTYAAHLSGAAAAIGILTPNPDERWSIMFDAGQAAAYMQL
                     AAWELGIGSCPATIYQPEEARQLLGFPADLHLRVMLSFGFPADPGELTASPHPGGRKA
                     LSQLVSFERWQDPLP"
     gene            812..1915
                     /locus_tag="VI520_02050"
     CDS             812..1915
                     /locus_tag="VI520_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF36695.1"
                     /translation="MNTGYRMVYPFLPVIARSLEVAPAAIILGVTARSAVGLGTPLLG
                     ALADRIGRKPALLAGVAIFIAGGLMVALHPSYPTLVLALLSIGLSKALFDSALYAYVG
                     DRVPFERRGTSMALVEFSWSGAFLFGIPAIGLLIGSAGWVAPFPALAGLAVLGGIWIW
                     TTLPGNSGSGVRGPSVASGFSAIVARKSALAGLAVGLLISAANETINIVYGLRMEQAF
                     GLRIAALGAATAVIGLAELGGEGLVAGLADRIGKRRLVAVGIVASTGTALMLPLLAGS
                     LGVGLLGLFAFYLSFEATIVGSLPLMSEQVPQARATLLATNIAGLSLGRSMGALAGAP
                     LFALGIQANAAVAAALNLLALGLLLGLVKEQGN"
     gene            1918..3207
                     /gene="hutI"
                     /locus_tag="VI520_02055"
     CDS             1918..3207
                     /gene="hutI"
                     /locus_tag="VI520_02055"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HLF36696.1"
                     /translation="MESRPTVDLLVHSAAQLLTCSGGPQRGPALGTLGLIEHGAVAIR
                     AGLIVATGSTEELRRSFSASRELSAAGRVVLPGFVDPHTHLVWAGDRAAEFEQRVQGA
                     SYLEILEAGGGILSTVRRTRFANLETLLAESRPRLRKMLEHGTTTAEAKTGYGLELEA
                     ELRLLHAILALGSEGPVDLVPTFLAAHAIPEGWQPAEYVALICDHMLPALVPWWARNG
                     QGRPLPFVDVFCEVGAFDLELSRRVLECARSLGFPLKIHADEFNPLGAASLAVELGAT
                     SADHLLQTPASELKRLAGSDTVAVALPATPFGLGVPTYAPAQAILQHGGILALGTDLN
                     PGTAWCENMQFVISLACRTMGLSPAQAISAATINAAAAIGRDERVGSLEPGKEADLLL
                     LSVSDYRQLGYRFGTNLVSTVIKKGRIVVAEKTDGLD"
     gene            3194..3370
                     /locus_tag="VI520_02060"
     CDS             3194..3370
                     /locus_tag="VI520_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36697.1"
                     /translation="MDWTEAPLAVRVVAIVAAAVAAWISIRFVLRITLRLFALGCLGL
                     AVLVVIGGVAGWLG"
BASE COUNT          551 a         1164 c         1153 g          686 t
ORIGIN      
        1 cggcagctgt ccgcgctgca gccctgagat ccgatcctga tcggtgtcca gggccacgac
       61 ccggttcccg agatcggcaa agcacgctcc ggtcaccaag ccgacatggc cgaccccgac
      121 caccgtcaga ttcttcaaaa gcgcaagctc ctagaggggg cggcaataat accatgcaac
      181 cccgcccacg agcctagctg gcgtaaaatc atctccgtca tcccgccctg gagggcgccg
      241 tggaagtcac cgaggccatc cgattgaaac gagctgtgcg cagtttcgcc cccgaggcac
      301 ttcccgagca gggcgtgcgg gccattctga acgccggtcg tagggcccag tcggccaaga
      361 accgccaggc ctggcacttt atcgtcatcc gcgaccggga gacgctgggg gagctggcca
      421 aacttggcac ctacgccgcc cacctatcgg gggccgccgc cgccatcggc atcttgaccc
      481 ctaaccccga cgaacgttgg tcaatcatgt tcgatgctgg gcaggccgcg gcctacatgc
      541 aattggccgc ctgggagctg ggaattggct cctgtccagc gaccatctac cagcccgaag
      601 aggctcgcca gctgctgggg ttcccggcgg atctgcatct gagagttatg ctttcattcg
      661 ggttccctgc cgaccccggc gagcttactg cctcgcccca cccgggcggc cgaaaggcgc
      721 tctctcagct ggtctccttt gagcgctggc aggatccctt gccctgaaaa tctccgttca
      781 actgatcccg ctcactctga cccggacggt catcaatacc ggttatcgga tggtctatcc
      841 cttcctgccg gtcatcgccc gcagcctcga agttgccccc gcggccatca tcctgggagt
      901 gactgcccga tcggcggtcg gcctggggac tccactgttg ggagcactcg ccgaccggat
      961 cggacgcaag ccggcgttgc tggcgggcgt ggcaatcttt atcgccggcg gactgatggt
     1021 ggccctacac ccaagctacc ccaccctggt gctggcattg ctctcgatcg gcctgtccaa
     1081 ggcgctgttc gactcggccc tgtatgccta tgtgggtgac cgggtgccgt tcgagcggcg
     1141 ggggacctcg atggcgttgg ttgagttcag ctggtcgggc gcgttcttgt ttgggatacc
     1201 ggctatcgga ttgctgatcg gttccgccgg atgggtggcc cccttcccgg cgttggcagg
     1261 cctagccgtc ctagggggaa tctggatctg gaccaccctc cccgggaatt ccggctctgg
     1321 cgtccgaggg ccctcggtcg ctagcgggtt ctccgcgatc gtcgccagga agtcggccct
     1381 cgccgggctg gcggtcgggc tgctgatctc ggcggccaac gagaccatca acatcgtgta
     1441 tggcctcagg atggagcagg ccttcggcct gcggattgcg gcgttaggtg cggccacggc
     1501 cgtcatcgga cttgccgagc ttggaggcga agggctggtc gcgggcctgg cagatcggat
     1561 tggcaagcgc cgactggttg cggtgggaat cgtggccagc actgggaccg cactgatgct
     1621 tcccctgctc gccggatcgc tcggggttgg cctgcttggc ctcttcgcat tctatttgag
     1681 cttcgaggcc accattgtcg gcagcttgcc gctgatgagt gagcaggtac cgcaggccag
     1741 ggcgaccctg ctggcgacca acatcgccgg actgtcgctc ggccgcagta tgggggccct
     1801 cgccggagct ccgttgttcg cgctcgggat acaggccaac gcggcagtcg cggcagccct
     1861 gaatctgctg gcattgggct tgctgctcgg cttggtgaag gaacaaggta actgaagatg
     1921 gaatcgcgac cgaccgtcga tctgctggtc cactctgctg ctcagctgct cacctgctcc
     1981 ggcggcccgc agcgcgggcc cgccctcggc accctggggc tcatcgaaca cggggcggtt
     2041 gcgattcggg ccgggctcat tgtagcgacc ggctcgaccg aagagctccg ccgctccttc
     2101 tccgcatccc gcgaactcag cgccgccggt cgggtggttt tgcccgggtt tgtcgacccc
     2161 cacactcacc tggtgtgggc cggcgaccgg gctgccgaat ttgaacagcg ggttcagggc
     2221 gcgagttacc tggagattct ggaggccgga ggtggcatcc tttcgaccgt tcgccggacc
     2281 cggttcgcca acctcgagac cttgctggcc gaaagtcgac cgcggctgcg caagatgctc
     2341 gaacacggaa cgacgaccgc cgaggccaag accggctacg ggctggaact ggaggccgag
     2401 ctgcgtctcc tgcatgcaat cctggcgctg ggttccgagg gaccggtcga cctggtgcca
     2461 acctttctgg cggcccacgc catccccgaa ggctggcagc ccgcggaata cgtggcgctg
     2521 atctgcgatc acatgctgcc ggccttggtt ccctggtggg cccgcaatgg ccaaggacgc
     2581 cccctgccat ttgtcgatgt gttttgcgag gttggggcct tcgacctcga gctgagccgc
     2641 cgggtgctcg agtgcgcccg ttcattgggt ttcccactta agattcacgc cgacgaattc
     2701 aacccgctgg gagcggccag cctggcggtc gaactcgggg ccacttccgc ggaccacctg
     2761 ctccagactc cggcttccga gctcaagcgg ctggcaggat cggacaccgt ggccgtggcg
     2821 ctgccggcca cgccattcgg cctgggcgtg ccaacctacg ctccggcaca ggccatccta
     2881 caacacgggg ggattctggc gctcggaacg gacctcaacc cagggaccgc ttggtgcgag
     2941 aacatgcagt ttgtgatcag cttggcctgc cgaaccatgg ggctcagccc cgctcaggcg
     3001 atctctgcgg ctaccatcaa cgccgccgcc gctattggca gggacgagcg ggtcggcagc
     3061 ctcgagcccg gtaaagaagc cgacctgctc ctgctgagcg tgtccgacta tcgccagctt
     3121 ggttaccggt ttggcacgaa ccttgtatcg acggtcatca agaaaggccg aatcgtcgtg
     3181 gctgagaaga ccgatggatt ggactgaagc accactcgcc gtccgggtgg ttgctatcgt
     3241 ggcggctgcc gtcgcggctt ggatctcgat tcgatttgtg ctcaggatca cactgcgcct
     3301 gttcgccctt ggctgcctgg gcctcgccgt gctggtggtc attggcgggg tcgccggctg
     3361 gctgggctga gtcaccctga agcggcgtaa ttctcggcac taaccaaaac agcccctcca
     3421 acacggaggg gctgttgttt tgagttccct gcggactagt agtccggcat gggtgggctg
     3481 ggtgtcttat cttcctgggg aacgtcggtc accagggcct cggtcgtgag aatcatggcc
     3541 gcgatcgagg cggc
//
LOCUS       DATDPQ010000091        10252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_813194, whole genome shotgun sequence.
ACCESSION   DATDPQ010000091 DATDPQ010000000
VERSION     DATDPQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813194"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VI520_02065"
     CDS             complement(<1..930)
                     /locus_tag="VI520_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLF36698.1"
                     /translation="MAEKDPVRLVRQALPDEGLSDPRIIVRDEPAGPERRPPDAPELA
                     LPTELPILPLRGVVVYPQTVVPLTIGQPRSVRLVDDVVGADRMVGLVASRRPELELPG
                     PEDLYSIGTLATVQRLFRAPDGTIRMIVQGLSRFSLDEFTSVEPYLKARIVLRPETVE
                     EGVELEAQVRAATDQFQEVAELAPSLPREVVASVMLQEDPLQVAYTIANYQRMDLEVA
                     QQLLEDDSTLAKLQKLNGLLSREIEVLSLGQQIQEEAREEIEKVQREYFLREQLKAIQ
                     RELGEVDEQAAEVEEFRAKLEKAGLSPEADKMAR"
     gene            complement(940..1362)
                     /locus_tag="VI520_02070"
     CDS             complement(940..1362)
                     /locus_tag="VI520_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLF36699.1"
                     /translation="MPRNQLVDPYELSAIEDDSWPPKSEHPSLWGVKRRTHKWRPPTD
                     VYETDDAYQVRVELAGMRGADIAVTFERELLVIQGVRSDSGPRKEFRQLEIAYGEFET
                     TIQIPEPVEVSKIEAAYQDGFLRVELPKLKPRRVSIEE"
     gene            1518..1958
                     /locus_tag="VI520_02075"
     CDS             1518..1958
                     /locus_tag="VI520_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLF36700.1"
                     /translation="MPEILSQVELFEGLSRIELEQIASICKEQSFRSGDVVTRQGDTG
                     DQLFVIWDGLVEVVHEVAPHDPAPRTVVNLGQGQVFGEMALVDYGPRSATIRVISDRA
                     KVQSIQRDAFMELCESQTHLGFVVMRNIAADLSFKLRHQHLVGR"
     gene            1962..2165
                     /locus_tag="VI520_02080"
     CDS             1962..2165
                     /locus_tag="VI520_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36701.1"
                     /translation="MTAIFKVSYVVRGGGHPGAIINEEQRPQVGQRVKLGAQEFEVIE
                     VVDLMPARGDFSFLHVTLQPPKL"
     gene            complement(2195..3634)
                     /locus_tag="VI520_02085"
     CDS             complement(2195..3634)
                     /locus_tag="VI520_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HLF36702.1"
                     /translation="MSSREPSWLPGTLTVARREIRDQLRDWRILTPIVLLTLFFPILM
                     NFTANQAVRFVEQYGAEIIADRLIPFLMMVVGFFPISISLVIALESFAGERERLSLEP
                     LLATPLSDAQLYFGKIIASMLAPLGAAYLGIFVYLFGLYWRLGWQPPLPLLAQVLLLT
                     TAQSIVMVTGAVVISSQTTSVRAANLLASFIIVPVSQLIIGESLIMFWGRYSVLWWIV
                     AALALVAIVLVRMGLHLFNREQLLGRDFDDFDLKWVMSSLWAGFRGRAQTLSGWYREL
                     LGETLPQARVSILIMILALLVAYGVGYRAASDFVLPAELFQGERLQDALGDELSSFGL
                     LSARGWLWVAWNNVRALGLASLLGAFTFGVAAVVLLMAPLGIVGYFAGSFALAGGSAA
                     RILGGLVLPHALLEIPAAVLAGAAIVQLGLAALSLPKGSSLGASWLEALGRWARLSLG
                     LVLPLILVAAAVEVFLTPRVAIAVLAGLP"
     gene            complement(3631..4563)
                     /locus_tag="VI520_02090"
     CDS             complement(3631..4563)
                     /locus_tag="VI520_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36703.1"
                     /translation="MTPLIETFKLGKRFGSLVAVDQVSLRVDAGEVLALLGPNGAGKT
                     TTIRMLASILGPTHGWVRIAGLDTVTHASQVRRLVGMLTEHHGLYTRMRAIEYLAFFG
                     EIYGMPTAQVRERSHGLLDRFGLIETAELRLGAYSKGMRQKLAMARALLHDPTVLLLD
                     EPTSAMDPESARLVRESIRGLRAGNRAIVVCTHNLPEAELLADRIAVIRRGRIVAHGS
                     PEELKREFLGSPVMELRVAGGLDGAVRLLPQGATLRETGPNWLRFQSADPETVNPAVI
                     RAMGRAGIEIVTLSEIGRSLEEVYLSVMQSESSD"
     gene            complement(4643..5497)
                     /gene="lgt"
                     /locus_tag="VI520_02095"
     CDS             complement(4643..5497)
                     /gene="lgt"
                     /locus_tag="VI520_02095"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HLF36704.1"
                     /translation="MNVDFQPVGIQITALGLSFFLRYYGMLLMLGALAAAAVTSRLLK
                     IDRADSEIAWDGLIWVLVFGVVGARLYHVFTPSLSLQEQGITTAWYLTHPLDLINTTR
                     GGLAMPGAIMGGVFGLYLFARRRKLPLGKLMDAAAPGVALAQAIGRWGNYVNQELYGP
                     PTDLPWGIRIDPANRLPGYEPFERFHPLFFYESIWNLVNFIFLLWLWRNYRDRLVSGD
                     IFLAYLVSYPLGRFLLEFLRLDYVPAFGINFNQAVMLVVAIAAAVALFLRNRPVPVHP
                     RKRRPKQA"
     gene            complement(5487..6113)
                     /locus_tag="VI520_02100"
     CDS             complement(5487..6113)
                     /locus_tag="VI520_02100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLF36705.1"
                     /translation="MDQTQPRTLSDFMRARFRGVVDGIGMVLKDLGVTPIALSLVGLA
                     GHLVAAVLVARGEFLIGGLFLLFLAPVDALDGALARLRGETSNFGGFFDSVIDRYSEM
                     LVFGGLLIYFQNRFDKGMVSLVFVAAAGSLLVSYTRARGEALGYVVRSGMLTRFERYL
                     ILIPSLLFGLPKWGVGIIAVLANLTAVQRFLDVRQQAWNKKEKAFRER"
     gene            complement(6163..8217)
                     /locus_tag="VI520_02105"
     CDS             complement(6163..8217)
                     /locus_tag="VI520_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HLF36706.1"
                     /translation="MRIARRSLWDLLAIPLLAAAVVTSLGLLDLSAGRLLDAWIGFLR
                     RWLGLGAMVVPVTAALAILIRPGRWDRVIATELVFFSVLGLLSAVAADSLPEAELGRG
                     GGIVGWAIADTLGRIVGSVGRILALLLIGASATALALGDRLGRRLPAGPIVASPPGAA
                     PPAEAAKAPGKRRVPAEFRKEFKLPEASNSEKAGSRARSELPASLDFLEPGETRQVTA
                     REINLAAGLIEKTLAEFGLPAKVVDFRSGPSVTQFAVEPGYVEHQAPDGSVSRSKVRV
                     SQISSLANDLALALSAPAIRIEAPVPGKAYLGIEVPNRKPERVRLRPILESERFRAMG
                     SPLAIALGRDVAGESIVADLASMPHLLIAGTTGSGKSVCITALAACLAINNGPEQLRF
                     VLIDPKRVELLRFNGLPHLMGKVETDLTRITGVLRWCTLEMDRRFKLLEAASARDVAI
                     YNRKMLRRSGGEALPRLVVMIDELADLMMLAPDATESALVRLAQMARATGIHLVVATQ
                     RPSTDVVTGLIKANFPARIACAVASGIDSRVILDGPGAESLLGKGDMLYLAPEAAAPV
                     RLQGVYVSDKELEGLISSWNSGAARGALAPWEDFLVRQDALSQQDDVLERAIELVKET
                     GQASASLLQRRLRVGYPRAARLMDELEELGLIGRATGGGRTREVLMSDDDRGDEPAGP
                     QP"
     gene            complement(8272..>10252)
                     /locus_tag="VI520_02110"
     CDS             complement(8272..>10252)
                     /locus_tag="VI520_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36707.1"
                     /translation="VLSGRGNRAFLHPESEVAGSMLETLTSALMVVNQILNAGNAVTA
                     FSLLLYSLTFNPKERTARILALLLGCLALVYATDVMAGTATLESEQQIWLRLQWAGIS
                     FGAAGYMHISDALLAATGRPSRGRRKWIIRIGYLLSLVSFGSAALSEELVGPLNIVGP
                     VAFLSGGRLLPLFVAYFGLALTFTGFNFWRAYKRCLTSVSRRRMRYLIFGSAAPFLGV
                     FPFLSAGGSFLLQTPLLTWAVLGVLNVLVAVQIVMMTYAVAYFGVSFPDRVVKSRLFQ
                     WLLRGPIVASTTLAVTVVIDRLGQRIGLENSRAVPLFMVGTILSLQYLITLIRPTVER
                     WLFYGQDRNDVSRLHLLEERLLTTGDLRQYLEAILNAACDLTGASSAFVAAVGEQGVE
                     LEVAVGPDDPLRDPLDSTPSLSAENNRELPGFGPLFRWDRYWLAPLLASPTGELVGVL
                     GLKLASGDFEFGPETVEALSLVRERAAVALTDRALQREVFAAVDRLVPQVEAVQRMRA
                     AARYGGVQALTMPAESLPADSDLANLVRGALGHYWGGPRLTQSPLLRLRVVREAVAAH
                     DGNPVNALRATLRQAIDRVRPQGERRFTGEWMLYNILEMKFLEGRKVRDVAVRLAMSE
                     ADLYRKQKVAIEYVARAMEEMEREARAAKPVNPRS"
BASE COUNT         2098 a         3331 c         3060 g         1763 t
ORIGIN      
        1 ccgggccatc ttgtccgcct cgggcgaaag tccggccttc tcgagtttgg cgcggaattc
       61 ttcgacctcg gccgcttgtt cgtcgacttc gcccagttcg cgctgaatcg ccttaagctg
      121 ctcgcgcagg aagtactcgc gctgcacctt ctcgatctct tctcgggcct cctcctggat
      181 ctgctgcccg agcgagagca cctcgatttc tcggctcagc aaaccgttga gcttttgtaa
      241 ttttgcgaga gtggagtcgt cttccagcaa ttgctgggca acctccagat ccatccgctg
      301 gtaattggcg atcgtgtagg cgacttggag cgggtcttcc tgcaacatga cgctggccac
      361 gacttcccgc ggaagcgagg gggccagctc cgcaacttcc tggaactggt cggtcgcggc
      421 ccgcacctgg gcctcgagtt ccaccccctc ttcaacggtc tccggcctca acacaatccg
      481 ggctttgaga tatggttcca ccgaggtgaa ctcgtccagc gagaagcggg acagtccttg
      541 cacgatcatt cggatcgttc cgtccggggc acgaaacaat cgttgcaccg tggccagcgt
      601 cccgattgaa tagaggtcct cgggtccggg aagttccaac tcgggacgac gggaggcgac
      661 cagcccgacc attcgatctg cgccgacgac gtcgtcgact agccggaccg aacgcggttg
      721 accaatggtc aacggaacga ctgtctgggg ataaaccaca actccgcgca gcggaagaat
      781 ggggagttcg gtggggagcg ccagctctgg ggcatcggga ggccgccgct cgggccccgc
      841 gggttcgtcc cgaacaatga ttctcgggtc gctcaaccct tcatctggca gggcttgccg
      901 caccaatcgg acaggatctt tctcagccat gaatccggat cactcttcga tcgagacccg
      961 ccttggcttg agtttcggga gctcgactcg gaggaacccg tcttggtagg cggcctcgat
     1021 cttggagact tccaccggct cggggatctg gatggtggtc tcgaattcgc cgtaggcgat
     1081 ctccaattgc ctgaactcct tgcgtggccc ggagtcgcta cggaccccct ggatgacaag
     1141 cagctcgcgc tcgaaggtta cggcaatgtc cgcacctcgc atgccggcga gctcgacccg
     1201 aacctggtaa gcgtcatcgg tctcgtagac gtcggtgggc gggcgccact tgtgagtgcg
     1261 ccgcttcact ccccaaagcg aggggtgctc ggactttggc ggccaactgt catcctcgat
     1321 ggcgctgagc tcgtaagggt caacgagttg atttcgtggc atgctcggcc tcggagaacg
     1381 tatttatgtt ctgttccgga cgcaatgttt cattctacca tcctcaccct ttcggcgcct
     1441 tcgccgaggc cgcggttgtc tgggtgtagg aatgccggta gaatgctcca gacggctccg
     1501 gaggcgctcg gatgaatttg cccgaaattt tgagtcaggt tgagctcttt gagggtctga
     1561 gccggattga actcgagcag atcgcctcaa tctgcaaaga gcagtcattc cggtcgggag
     1621 atgtggttac tcggcagggc gataccggcg accagctgtt cgtcatctgg gacggcctgg
     1681 tggaggttgt tcacgaagta gcgccccatg atccggcccc tcgaacggtc gtcaatctgg
     1741 gccagggcca ggtgttcggc gagatggccc tcgttgatta cggcccccgt tctgccacca
     1801 tcagggtgat ctcggaccgg gccaaggttc agtcgattca gcgggatgct ttcatggagt
     1861 tatgcgaaag ccagactcac ctgggtttcg tcgtgatgcg gaacatcgcg gccgacctgt
     1921 ccttcaagct tcggcatcag catctggttg gccgctagcc gatgaccgcc atcttcaaag
     1981 tcagctacgt cgtccgaggt gggggccacc ccggtgcgat catcaacgaa gaacagcgcc
     2041 cccaggtcgg ccagcgagtt aaattaggag ctcaggagtt cgaggtcata gaggtcgtgg
     2101 atctcatgcc ggcccgcggc gacttcagct ttctgcacgt caccctgcaa ccccccaagt
     2161 tgtaatcgcg cagacttgcc gcggtctgaa ctcgttacgg cagcccggcc agcactgcta
     2221 tggccactcg cggggtgagg aaaacttcaa ccgcggcggc caccaggatc aaaggcaaga
     2281 ccaagcccag actaagccgg gcccacctcc ccagcgcctc taaccaggag gcccccagcg
     2341 atgaaccctt cggcaaactc agcgcggcaa gcccgagctg gacgattgcc gcgccggcca
     2401 gcacggcggc ggggatctcc agaagcgcat gcggcaggac aaggcctccc aatatgcggg
     2461 ccgcactgcc gccggcaagt gcaaaactcc cggcaaagta gcccacaatt ccaagcgggg
     2521 ccattaacag gactacggcc gccacaccga atgtgaaggc cccgagcagg ctggccaaac
     2581 ccaaggcccg aacgttgttc caggccaccc acagccaccc ccgtgctgac agcaagccga
     2641 agctggacag ctcatcaccc agggcatcct gcagcctctc cccctggaac aattcggctg
     2701 gcagcacgaa atcgcttgcg gcccggtagc cgaccccgta cgccaccaaa agcgccagga
     2761 tcatgatcag gattgacacc cgcgcttgag gcagagtctc acccagcagt tcccgatacc
     2821 agccactcag ggtctgggcc cgccctcgaa acccggccca caagctgctc atgacccact
     2881 taagatcgaa gtcgtcgaaa tcccgcccaa gcaactgctc ccggttgaac agatgcagcc
     2941 ccattcgaac caacacgatc gcgacaagtg cgagagccgc cacgatccac catagaacgg
     3001 aatagcgtcc ccaaaacatg atcagacttt ccccgatgat caactgcgag accggaacga
     3061 taatgaagga ggcgagaaga ttggcagccc gcaccgaggt cgtctggctc gagatcacca
     3121 ctgctccggt gaccatgacg atggattggg cagtggtcag cagcaaaacc tgcgccaaca
     3181 gaggtagggg cggctgccaa cccagtcgcc aatagaggcc gaataggtag acgaatatcc
     3241 ccaggtatgc cgcgccgagg ggcgcgagca tcgaagcaat gattttgcca aagtaaagtt
     3301 gggcgtcgct gagcggcgtc gccagcaatg gctcgagcga gagccgctcg cgttcccccg
     3361 cgaagctttc caaggcgatt accagcgaga tggagatcgg gaagaagccg acgaccatca
     3421 tcaggaatgg gatcaatcga tcggcaataa tctccgcccc gtactgctcg acgaaccgca
     3481 ccgcctgatt ggcggtgaag ttcatcagga tcggaaagaa cagagtgagg aggacgatcg
     3541 gggtaaggat ccgccagtcc cgcagctgat cgcgaatctc ccttcgcgct acggtgaggg
     3601 ttccgggaag ccaggaaggc tcgcggctgc tcaatccgaa ctctccgact gcatgaccga
     3661 aaggtaaacc tcttccaagc tgcgaccgat ctcagagagg gtcacaatct caatcccggc
     3721 tcttcccatg gcccgaatga cggccgggtt gacagtctcc gggtcagccg actggaatcg
     3781 aagccagttt gggccggtct cgcgcagggt agccccctgc gggagcaggc gaactgcccc
     3841 atctaggccg cccgccactc gcagctccat gacgggactg ccaaggaatt cgcgcttcaa
     3901 ctcctccggc gatccgtggg ccacgatcct gcctcttcgg atgactgcaa tccggtcggc
     3961 cagcagctcg gcctccggca ggttgtgggt gcacaccacg atcgcccggt tccctgcgcg
     4021 caggcctcgg atgctctcgc gcaccaaacg agcgctctct ggatccatcg ccgaggtggg
     4081 ctcgtcgagc agcaatacgg ttgggtcatg gagcagcgcc cgcgccatgg cgagtttttg
     4141 acgcataccc ttcgaatagg ctccaagtct tagttcggcc gtctcaatca ggccgaatcg
     4201 gtcgagcagg ccatgggagc gctcgcggac ctgggcagtc ggcataccgt aaatctcgcc
     4261 gaagaacgcc agatactcga ttgccctcat gcgagtgtac aggccatgat gttcagtcaa
     4321 catcccgacc agccgccgga cctgactggc atgggtgacg gtgtccagac cggcgatccg
     4381 cacccaaccg tgggtgggcc caagaatgga agccagcatc cgaatggtgg tcgttttgcc
     4441 cgccccgttg gggccgagca aagcgagcac ttcccccgcg tcaactcgca gggagacctg
     4501 gtcaaccgcg accaggctgc cgaaccgttt tcctagctta aatgtctcga tcaggggagt
     4561 cattgggccg acccgcgcat tatagcaggc tggccctttc ggactcaatc ggcccgagtt
     4621 cccagacgaa tggcggccag gcttaggctt gcttggggcg gcgcttccgg gggtggaccg
     4681 ggacaggccg attgcggaga aaaagggcaa ccgccgcagc gatcgccacg accagcatca
     4741 cggcctggtt aaagttgata ccaaatgcag ggacgtagtc gagccgcaag aactcgagta
     4801 gaaagcgacc cagcggatag ctgaccaggt aggcgaggaa aatgtcaccg gagaccaggc
     4861 gatctcgata gtttctccac aaccagagca ggaagatgaa gttgaccagg ttccaaattg
     4921 actcgtagaa gaagagcggg tggaaacgct cgaacggctc gtagcccggc aatcgattgg
     4981 ccgggtcgat ccgaatcccc cagggtagat cggtcggggg cccgtagagc tcctggttga
     5041 cgtagtttcc ccagcgccct atagcctggg ccagcgccac tccgggagcg gcggcgtcca
     5101 tcaacttgcc aagcggcagc ttccgtcggc gggcgaaaag gtaaaggccg aacaccccgc
     5161 ccatgatcgc gcctggcatc gccagtccac cccgagtcgt attgatcaag tcgagcggat
     5221 gggtcaagta ccaggcggtc gtgattcctt gctcctgcaa actgagcgaa ggcgtgaaga
     5281 cgtgatagag ccgggcccca acgaccccaa agactagcac ccagatcaat ccgtcccagg
     5341 cgatttctga gtccgcccgg tcgattttta gcagtcggct ggtcacggca gccgcggcga
     5401 gcgcaccgag catcagcagc atcccgtagt accgcaggaa aaaactcagc cccaaggcgg
     5461 tgatctggat cccgaccggc tggaagtcaa cgttcacgaa acgccttctc cttcttgttc
     5521 caggcctgtt ggcggacgtc cagaaaccgt tggaccgcgg tgaggttggc caacaccgcg
     5581 atgatcccca ctccccactt ggggagacca aacaagaggc tcgggatcag gatcaagtac
     5641 cgctcgaagc gggtcagcat cccgctccgg actacatacc caagcgcctc cccccgggct
     5701 cgtgtgtacg agaccagcaa cgaaccggcg gccgcgacga acaccaggct gaccatccct
     5761 ttgtcgaatc ggttctggaa atagatcaac aaaccaccga ataccagcat ttcagaatat
     5821 cgatcgatca ccgaatcaaa gaacccccca aaattcgagg tctcgccccg caggcgggcc
     5881 agcgctccgt ccaatgcatc aaccggtgcc agaaatagca agaacagtcc gccgatcagg
     5941 aattcgccgc gagccacgag gaccgccgct accaggtgcc cggccagacc aaccagcgag
     6001 agcgcgattg gcgtgactcc caggtccttg agcaccatcc cgattccatc gaccactccg
     6061 cggaatcggg cgcgcatgaa atcgctcagg gttctaggct gggtttggtc catggctggt
     6121 ggcacgctgg attcgaaatg ctaacccgcg ggtatgggcc agtcaaggtt gagggccggc
     6181 aggttcatcc ccccggtcgt catctgacat caaaacctcg cgggtccgac ccccgccggt
     6241 ggctcggccg atcagcccga gctcctccag ctcatccatg aggcgggccg cccgcgggta
     6301 gccgacccgc aaccggcgct gcagcagcga ggcgctggcc tgcccggttt ccttcaccaa
     6361 ttcgatggct cgttccaaga cgtcgtcttg ctggctcagg gcatcctggc gcaccaagaa
     6421 atcctcccaa ggtgccaggg caccccgagc ggcccccgag ttccagctgc tgatcaatcc
     6481 ttccagttct ttgtcgctca cgtacactcc ctgcaaccgc accggtgcgg ctgcctccgg
     6541 ggcgagatac agcatgtcac ctttgcccag cagcgattcg gcgcccggcc catccaggat
     6601 cacccgtgag tcgatgccag aggcgaccgc gcaggcgatc cgggccggga agttcgcttt
     6661 gatcaagccg gtcaccacat cagtgctcgg tcgttgggta gccaccacca gatggatccc
     6721 ggtcgccctg gccatctgtg ccagccggac cagcgccgat tcggtcgcgt ccggtgccag
     6781 catcatcaag tccgccagct catcgatcat gacgacaagc cggggcagtg cctcgccgcc
     6841 gcttcggcgg agcatcttgc gattgtagat cgccacatcc cgggcagagg cggcctccag
     6901 cagcttgaag cggcggtcca tttccaacgt gcaccagcgc agcacccctg taatccgggt
     6961 gaggtcggtc tcaactttac ccatcaaatg cggcaggccg ttaaaccgca gcagctcgac
     7021 tcgcttcggg tcgatcagca cgaaacggag ctgctccggt ccgttgttga tcgccaggca
     7081 ggccgccagg gcggtgatgc acaccgactt gcctgaaccc gtagtgccgg cgatcagcaa
     7141 gtgcggcatc gaagccaggt cggcgacgat cgattcgccg gcgacgtcgc ggcccagggc
     7201 gatcgccagc ggggagccca tggcacggaa ccgctccgat tccaagatcg ggcgcagccg
     7261 aacccgctcc ggtttccggt tcgggacctc aatcccgagg tacgccttgc cggggacggg
     7321 agcctcgatt cggatcgccg gggcagagag cgccagggcc agatcgtttg cgagcgagga
     7381 gatctgcgat acccgcactt tgctgcggga caccgatcca tcgggtgcct ggtgttccac
     7441 gtagcctggt tcgactgcga attgggtgac cgaggggcca gatctgaaat caacgacctt
     7501 ggccggcaga ccgaattcgg ccaaggtctt ctcgatcaga cccgccgcca ggttaatctc
     7561 tcgggcagtc acctggcggg tctctccggg ttcgaggaaa tcgagcgagg ccggcaattc
     7621 gctccgagcc ctggagcccg ccttctccga attcgaggcc tccggcaact tgaattcttt
     7681 gcggaattct gccggtactc gccgcttgcc aggtgccttg gcagcctctg ccggcggggc
     7741 cgcgccaggc gggctggcga cgatcggacc tgccggaagc ctgcgaccaa ggcgatcacc
     7801 gagggccagc gcagtggcac ttgcaccgat cagcagcaaa gcaaggatcc ggcccaccga
     7861 ccccacgatt cgacccaacg tgtcggcaat cgcccaccca acgatcccgc cccccctgcc
     7921 caattccgct tccggcaggc tgtcggccgc aaccgcgctt agtagaccga ggactgaaaa
     7981 gaagacgagt tccgtggcga tgacccgatc ccagcgtccg ggacggatga gaatcgccaa
     8041 tgccgcggtg accgggacga ccattgcccc aagtccgagc caacgacgca ggaacccgat
     8101 ccaggcgtcc agcagccttc cagcactgag gtccagcagt ccgagagatg tgacgactgc
     8161 cgcggcaagc agcggaatgg caagcaagtc ccaaagcgat cgtcgggcga ttctcatgct
     8221 gacgggatca gagtgtcctc tggcggtctg aaaaaggcaa ggcccgccgc ctcagctgcg
     8281 cggattgacc ggcttggccg ccctggcctc ccgctccatt tcctccatcg cccgggcgac
     8341 atactcgatg gcgactttct gcttccgata taggtccgcc tcggacattg ccaggcggac
     8401 agccacatcg cggaccttgc gtccttcgag gaacttcatc tccaggatgt tgtatagcat
     8461 ccactccccg gtgaaccgcc gctcaccttg cggtctgacc cgatcgatcg cctgccgcag
     8521 ggtcgcacgc agcgcattga ccgggttgcc gtcgtgcgcc gccactgcct cgcgcacgac
     8581 cctcaaccgc agcaaggggc tctgggtcag ccgcggtccg ccccaatagt gaccgagggc
     8641 tcctcgaacc aagttggcca agtcggaatc ggccggcagg ctctccgccg gcatcgtcag
     8701 cgcttgcacc ccgccatagc gggcggccgc ccgcatccgc tgtacggctt ccacttgcgg
     8761 caccagccgg tctacggccg cgaacacttc ccgctgcaag gcccgatcgg tgagcgcaac
     8821 ggcggcccgc tcccggacca gcgacagggc ttcgaccgtt tccggcccga actcgaagtc
     8881 ccctgacgcc agcttcagcc caaggacccc aaccagttcg cccgtcggcg aggccagcag
     8941 cggcgccaac cagtagcgat cccagcggaa gagtggccca aatccgggca gctcgcgatt
     9001 gttttctgca gacaggctgg gggtcgaatc caacggatcg cgcagcggat cgtcgggccc
     9061 caccgccacc tcgagctcaa ccccctgctc gcccaccgcg gcaacgaacg ccgaggaggc
     9121 gccggtgaga tcgcaggcag cattcaagat cgcctccaga tactggcgca gatcgccggt
     9181 ggtgagcaac cgctcttcaa gcagatgcaa ccggctgacg tcgttacggt cctgaccata
     9241 gaaaagccac ctttccacag tcgggcgaat cagggtgatc aggtattgca ggctcaaaat
     9301 ggttccgacc atgaagagcg gaacggctcg gctgttttcc aagccgatgc gttggccgag
     9361 ccgatcgatg accactgtta ctgcgagggt cgtcgaagca acgatcgggc ctcgcaacaa
     9421 ccactgaaac agccggctct tcactacccg gtccgggaaa ctcactccga agtaggccac
     9481 cgcataagtc atcatcacaa tctgtaccgc aactaacaca ttgagcactc caagcaccgc
     9541 ccaggtcagc agcggggtct ggagcaggaa gctgcctccg gcactgagga atggaaacac
     9601 tcccaagaac ggcgccgcgg atccgaagat caggtagcgc atacgccggc ggctgacgga
     9661 agtcaagcac cgcttgtatg cccgccagaa attgaatcca gtgaaggtca gggcgagtcc
     9721 gaagtacgcc acgaagagcg gaagtagcct cccgccggag aggaaagcca ccggccctac
     9781 aatgtttagc gggccgacca actcctcgct gagggccgcg gacccaaagc tgaccaggct
     9841 taggaggtaa ccaattcgga tgatccactt ccggcgcccg cgcgagggcc ggccagtcgc
     9901 tgccagcaac gcgtcggaaa tatgcatgta gccggccgcc ccgaagctga tcccggccca
     9961 ttggagccgc agccagatct gctgttcgct ctccagggtg gccgtccccg ccatgacatc
    10021 ggtagcgtaa acgagggcca ggcagccaag cagcagagcc aggattcggg ccgttcgctc
    10081 tttggggttg aaggtgaggg aatagagcag cagggagaac gcggttaccg cgttgccggc
    10141 attcaggatc tgattcacca ccatcagggc ggaggtgagg gtctcgagca tgctcccggc
    10201 tacttcagat tcagggtgaa gaaaagcgcg atttcctcgt ccggatagta ct
//
LOCUS       DATDPQ010000092         3784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1342493, whole genome shotgun sequence.
ACCESSION   DATDPQ010000092 DATDPQ010000000
VERSION     DATDPQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342493"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3767)
                     /locus_tag="VI520_02115"
     CDS             complement(<1..3767)
                     /locus_tag="VI520_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36708.1"
                     /translation="MLRIALVQRVRRIAIQALAELRERQLADFWSNRLLVTARREPGL
                     LFARLAELAEAQPAPTPFFGTLLAAQLYDEEGVLGPVQSWLERAHHRPMSETNLREQN
                     RQATEQVTVANAITTLRQLAMLDWRELFEKLSRVDRALSRDPAGVYAGMDFETRNTYR
                     GVVEELARRAGRQEVDVARAAVELATQAARQAGSREGPPPASRPLDGGERSGHVGAFL
                     VGESRAALTKLLNFREPVGYRLRQFIYRHHTGVYLTAVAASTSLLALLPLAVGLQVKL
                     RWVDLIGGLLVLGPASQLGLQLVNYLVSRMLPPRTLPKMDFSRQGIPDAYRTLVVVPI
                     ILSDPETIPSEIEKLEIRYLANPESNLAFGIFSDFADAPEPQLPEDAATLARAVEGIE
                     RLNVRYGAGRFYLFHREREWSASERKYIGWERKRGKLEELNRLISGGTPAAPEHFVRV
                     GDPDDLTDIRFIITLDSDTQLPRDTARRMVETLAHPLNQARFDGQGMLLPGTYAIIQP
                     RVSPSLPSATATAFSRTYSDPVGVDPYTKAVSDTYQDLTGAGSYHGKAIYDPRAFHRV
                     LSDRFPEQWLLSHDLIEGEHLRVGLASDIELFDEFPGDYLTYTRRQHRWIRGDWQIID
                     WLLPKVPSRSGGKEPNSLPLFSRWKILDNLRRSLVPLASVALLLVAWLNSSPMAWAAG
                     VLVAGMLLFQPVAQPLTWATSAQTLRSLSLRQIGHDLVRAIAEASLLPHQAGLALDAV
                     LRVWYRRLVSKRGLLEWTSASMAQYASAADQPLFILHSAAISLLAALLGLGLVANDPA
                     RLIPASPWIVLWILSPVLGWWLNRRPPEPNLSPAAVGPDLRMVRTVARKTWRFFDDFV
                     GTDTRWLPPDNYQVSHQNQLALRTSPTNVGMWLLSALAACDFGYLTLDQAVERLSKTF
                     DTLAQLERYQGNLLNWYDLRTLQPLRPRYVSSVDSGNFVAALWTLNQGLRDLAGSPVL
                     GPDALNGLLDTVEILESVLEQDGEAQLDRGEVAALSKRIRSSGTRLDDIVRCLREIET
                     PVRSLADSAREFAGTEARTAYWARQLERQLSAWIRVIDRYLSWVEPLPQANGAAQLAV
                     APSLSVLAGDWTPDLWPVLSANGPEPRPAGFDERLAACRTRAREAFGLVESLAGRADQ
                     LADQVDLGFLYDPQRRLFSIGLNISENRLDDAFYDLLASEARLGSLVAIARGQAPVEH
                     WLSMSRPFSSHGRHRVLLSWTGTMFEYLMPLLFQRTFPNSLLDKATREAVEL"
BASE COUNT          728 a         1170 c         1269 g          617 t
ORIGIN      
        1 agctcgaccg cctcgcgggt ggccttgtcg agcagcgagt ttggaaaggt ccgttggaac
       61 agaaggggca tcaggtactc gaacatcgtg ccggtccagc tcagcagcac ccggtgtcgc
      121 ccatgagacg agaatggtcg actcatggac agccagtgct caaccggcgc ctggccacgg
      181 gcgatcgcca ccagactgcc caggcgggcc tcgctggcca gcagatcgta gaaggcatca
      241 tcgagccgat tctcggagat gttcagcccg atcgagaata accggcgctg cgggtcatag
      301 agaaacccca ggtccacctg atccgcaagc tggtcggccc ggccggccag cgattcaacc
      361 aagccaaagg cctcccgggc gcgggtgcgg caggctgcga gcctctcgtc gaacccggct
      421 ggcctgggtt cgggcccgtt agcagagaga actggccaca ggtcgggggt ccaatctccg
      481 gccagcacgg aaagcgaggg agcgacggcc agttgagcag ccccgttcgc ttgcgggagc
      541 ggctcgaccc aggagagata gcggtcaatc acccgaatcc aggccgataa ctgccgctcg
      601 agctgacgag cccagtatgc cgtgcgcgct tcggtcccgg cgaactcgcg agccgagtcc
      661 gccaaagagc gaaccggagt ctcgatctcc cgcaggcagc ggacgatgtc atccaggcga
      721 gttcctgaac ttcgaattcg cttggagagg gcggccacct cgccgcgatc gagctgggcc
      781 tcgccatctt gttcaaggac cgattccaga atctccaccg tgtcgagcag cccatttagc
      841 gcatcgggtc ccagcaccgg gctgcctgcc aggtcacgca gaccctggtt gagggtccac
      901 agagccgcga cgaagtttcc gctgtcgacc gaggagacgt acctgggcct gagcggttgg
      961 agggtgcgca ggtcatacca attcagcagg ttcccctgat aacgttcgag ctgagccagg
     1021 gtgtcgaagg tcttggagag tcgttcgacc gcctgatcga gggtgagata gccgaagtcg
     1081 caagccgcca gcgcgctgag cagccacatt ccaacattgg tcgggctagt gcggagcgcc
     1141 agctggtttt ggtgggagac ctgatagtta tccggcggca gccagcgggt gtcggtaccg
     1201 acaaagtcgt cgaaaaaccg ccaggtcttg cgggccaccg ttctgaccat gcgcagatcg
     1261 ggaccaaccg cagcgggaga tagattcggc tccggggggc ggcgattcaa ccaccaaccg
     1321 agcacgggac tgagaatcca caacacgatc cagggtgagg ctgggatcaa tcgggcaggg
     1381 tcgtttgcga ccagcccgag accgagcagc gcggccagca ggctaatcgc agccgagtgc
     1441 agtatgaaaa gcggctggtc ggccgccgaa gcgtactgag ccatgctagc gctggtccac
     1501 tcgagcaatc ctcgcttgct cacgagccgg cgataccaga ctcgcagcac cgcgtccaaa
     1561 gccaatccgg cctgatgggg gagcagcgag gcctcggcaa ttgcgcgtac gagatcatgc
     1621 ccaatttggc ggagcgagag cgagcggaga gtttgggcgc tggtcgccca ggttagcggc
     1681 tgggcgaccg gttggaagag tagcatcccg gcaactaaga ctccggcggc ccacgccatg
     1741 ggagaggagt tcagccaggc gaccaagagc agcgccacgc tcgcaagcgg gaccaggctg
     1801 cggcggaggt tgtcgagaat cttccagcga ctgaataggg ggagggagtt cggttccttc
     1861 ccgccagagc gggagggcac cttaggaagc agccagtcga tgatctgcca gtctccccga
     1921 atccagcggt gctggcgccg ggtataggtg aggtaatcgc cggggaactc gtcgaacagc
     1981 tcgatgtcgg aggccaggcc tacccgcaga tgctcgccct cgatcaagtc atggctgagc
     2041 agccattgtt ccggaaagcg gtccgaaagc actcggtgga aggcccgcgg atcgtagatc
     2101 gccttgccgt ggtaggaacc ggcgccggtt aggtcttgat aagtgtcgga caccgctttc
     2161 gtgtagggat cgacgccgac cgggtctgag taggtgcggc tgaacgccgt ggcggtggcg
     2221 ctggggaggg aagggctgac ccttggttgg atgatggcgt aagtgccggg gagcagcatc
     2281 ccctggccgt cgaacctcgc ctggttgagc ggatgtgcaa gggtctcgac catcctgcga
     2341 gcggtgtcgc gcggcagctg ggtgtcgcta tccaaggtaa tgatgaaacg aatatcggtc
     2401 aggtcatcgg ggtcgccaac ccggacgaaa tgctctggag cagcgggcgt tccgccggag
     2461 atcagccggt tcagctcctc caacttcccg cgcttgcgct cccaaccgat gtacttcctt
     2521 tccgaggcac tccactcccg ttcccggtgg aatagataga agcggccggc gccgtagcgg
     2581 acgttcagcc gctcaatgcc ttcgactgct ctggctaagg tagccgcatc ctccggcagc
     2641 tgcggctccg gcgcatcggc aaagtcagag aagatcccaa aggccagatt cgactcggga
     2701 ttggccaggt agcggatctc cagtttctct atctctgagg ggatcgtctc cggatcggag
     2761 aggatgatcg ggaccaccac cagggtgcgg taggcgtccg ggatgccttg ccggctgaag
     2821 tccatcttgg gcagggtacg aggcggcagc atccgtgaga cgaggtaatt cactaattga
     2881 agcccgagct ggctggcggg cccgagcacc agcagcccgc cgatcagatc cacccagcgc
     2941 agcttgacct gcagaccgac ggccaatggc agcagcgcca gcagcgaggt ggaggccgcc
     3001 accgccgtga ggtagacgcc ggtgtggtga cgatagatga attggcgcag ccgatagcca
     3061 accggttcgc ggaaattgag cagcttggtc agcgcggctc tggattcgcc gaccaggaag
     3121 gcgccaacgt gcccggaccg ctcaccacca tccagcgggc gcgatgcggg cgggggccct
     3181 tctcggctgc ctgcctgccg agccgcctga gtggccagct cgaccgcggc tcgcgccacg
     3241 tcgacctcct gtcggccggc gcgccgggcc agctcctcca ccacaccgcg ataggtattg
     3301 cgggtctcaa agtccattcc cgcatacacg ccggccggat cacggctcaa ggcccggtcg
     3361 acccggctca gtttctcgaa gagctcccgc cagtccagca tcgccagctg gcgcagggtt
     3421 gtgatggcgt ttgccacggt tacctgctcg gtggcctggc gattctgctc gcgcaggtta
     3481 gtctcgctca tcggccgatg gtgggcccgc tcaagccaac tctgaaccgg tcccagcaca
     3541 ccctcttcgt cataaagctg ggcggcgagc agagttccga agaacggggt gggggccggc
     3601 tgggcttcgg cgagctcggc cagcctggcg aacaacaggc cgggttctcg acgggcggtg
     3661 accaacagtc ggttcgacca gaaatccgcc agctggcgtt cgcgcagctc agccagcgcc
     3721 tggattgcga tccgacgaac ccgctgcacg agcgcgattc gcagcatcag gggcagggcc
     3781 caca
//
LOCUS       DATDPQ010000093         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1860031, whole genome shotgun sequence.
ACCESSION   DATDPQ010000093 DATDPQ010000000
VERSION     DATDPQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860031"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VI520_02120"
     CDS             <1..491
                     /locus_tag="VI520_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36709.1"
                     /translation="LELEDIQVVGEAGNGWEALSLTETLNPDLILMDQNMPALSGIDA
                     TRRIKQARPDRRVLFIAAEEAWRQEALKAGAEGYFVKGEGFEQLIRAIHQPGLGGALQ
                     WQIDSRRHRRVLVRRVALTLGGLAVFALILVVFPLPPESFPVIALILASFSLVLYLIG
                     GD"
     gene            628..1398
                     /locus_tag="VI520_02125"
     CDS             628..1398
                     /locus_tag="VI520_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLF36710.1"
                     /translation="MISIVNRKGGVGKTTTAFNLAGGLAGRGNSVLLIDLDPMGSLCR
                     SLQIRPGELTLSDLLVGLNGSLGELIRRTEIPNLYCIPGDPNLRSFEMEHAASLAYRE
                     ALRDKLEGVLKWKPFPFVIIDCPPSLGLISGNALVASTDVIIPIDGSAYGMGALVDTL
                     RVLKLVRKNLNQQLSITGLLLNNVDLGTVYDKTVRDVMQRQFPGLLFQSVIPASPESD
                     VSSQLGQPVVHHSPESWMAKAYRQLIEEVITREPLRGA"
     gene            1388..2281
                     /locus_tag="VI520_02130"
     CDS             1388..2281
                     /locus_tag="VI520_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36711.1"
                     /translation="MGHNAHLLQESLLNALRTDPLLIRAEEFLHSAQPHETQELLEDN
                     LTRLLDPELAIAEIDRRQYRPMDSGMIERFRTAMEIAVSGGANLADAERQLLESFGLS
                     KVGELRAAMRTNLGAPAELPEQTLAIYVPEGDRKQEEFQRALRRDLQALETALEQPDA
                     DIAPAEPELAPPVEAAQEPAAEPVDIASDLRASLTLEADRQGSKSRGLAKLNPIPMLA
                     AALEAVGIGGEEFGFILRYGRYAPKGRNSQFLIGGLGLADLSRRLTAWLETNGPLEGQ
                     VQVIDEGSGELMVYLLLPEAA"
     gene            2316..>2881
                     /locus_tag="VI520_02135"
     CDS             2316..>2881
                     /locus_tag="VI520_02135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF36712.1"
                     /translation="MAGLNRAIRWLKNLGLWRTGGAVACLMLLAAILAFPSVVLPVVA
                     LLCGGLFFFYGVKSYASVAIIMLSTSGPTGNGKPNGDGQKGRRSGLLGRLKGGGRAHP
                     SRGSGGGKPYRLPPEKQPFISIQLPMYNENRVVDRLLAACTQLDYQNYEVLVADDSSD
                     ETLKTLERWAKHPKVKVSHRINRSGFKGA"
BASE COUNT          526 a          890 c          922 g          543 t
ORIGIN      
        1 tgcttgaatt ggaagacatc caggttgttg gcgaagccgg caacggctgg gaggcgctga
       61 gcctgaccga gaccctgaac cccgatctga tcctgatgga tcagaacatg cccgccctct
      121 ccggcatcga cgccacccgg cgaatcaagc aggccaggcc cgatcgccgg gtactgttca
      181 ttgcggcaga ggaagcctgg cgccaggagg ccctgaaagc cggagccgag ggctatttcg
      241 taaagggaga aggattcgag cagctcatcc gggccatcca tcagccgggg ctcggcggcg
      301 ccctgcagtg gcagatcgat agccgcaggc accgtagggt cctcgtacgc cgagtggccc
      361 tgaccttggg tggcctggcg gtgttcgcgc tcatcctcgt tgtattcccg ctgcctcctg
      421 agtcgttccc ggttatcgca ctgattctgg cgagtttttc gctcgtgctc tacctgatcg
      481 gaggagactg agatgcaaag agtggatcaa gcccccgcag ggttgctgag gcgccagctt
      541 gagccgacgc cggcaaacca attggcggtt ccacgtccag agcctgcggt caggcctgcc
      601 cccccggtcc cggccccctc gccgcgggtg atctcgattg tgaaccgcaa gggcggagtg
      661 ggcaagacca ccaccgcgtt caacctggcc ggagggcttg ccggaagagg gaattcggtt
      721 ctcttgatcg acctggaccc gatgggttct ttgtgccggt ccttgcagat ccggcccggc
      781 gagttgactc tttcagacct gttggtcggg ctgaacggca gcctcggcga gttgatccgg
      841 cgcaccgaga tcccgaatct ctactgcatt cccggggatc cgaatctgcg cagcttcgag
      901 atggagcacg cggcctccct ggcctaccgc gaggcgctgc gagacaagct ggagggggta
      961 ctgaagtgga aaccgtttcc attcgtcatc attgactgcc cacccagcct cggcctgata
     1021 tccggcaacg ccctggtcgc ctctacggac gtgatcattc ccattgacgg ttcagcctat
     1081 gggatgggtg ccctggtcga taccctccgg gtcctcaagc tggtacgaaa gaacctcaac
     1141 caacagctct cgattacggg tctgctgctc aacaacgtcg atctgggcac ggtgtacgac
     1201 aagaccgtgc gggacgtgat gcagcggcag ttcccgggcc tgctgttcca gtcggtcatt
     1261 ccggcctcgc ccgagtcgga tgtgagctcg cagttgggcc aaccggtcgt ccaccattcg
     1321 cccgaaagtt ggatggcgaa ggcctaccgc cagctgatcg aagaagtcat cacccgggag
     1381 ccgctccgtg gggcataacg cccatctcct gcaggagtcc ctgctcaacg cgttgcggac
     1441 cgatccgttg ctcatccgag ccgaagagtt cctgcattcg gcccaacccc acgaaacgca
     1501 agagctgttg gaagataacc tgacccgtct gctcgatcct gaactggcga tcgccgaaat
     1561 cgaccgccgg cagtaccgcc ccatggacag tggaatgatc gagcggtttc ggactgcgat
     1621 ggagatcgcc gtctccggtg gggcgaatct ggccgatgcc gagcggcagc tcctggaatc
     1681 attcgggctg agcaaggtgg gggaactgcg ggcagcgatg cggaccaatc ttggggcgcc
     1741 agcagagctc cctgaacaga ccctggcgat ctatgttccg gagggcgacc gcaagcagga
     1801 agagttccag cgggccctgc ggcgagacct tcaggccctt gagaccgcac tcgagcagcc
     1861 agacgcggac attgcaccag cagaaccgga gctcgctccg ccggtcgagg cggcccagga
     1921 accggcagcc gagcctgtgg atatcgcatc cgatctgcgc gcttcgctca ccctcgaagc
     1981 ggatcgtcag ggatccaaat cgagaggttt ggccaagctc aaccccatcc caatgcttgc
     2041 ggccgctctg gaggcggtcg ggatcggtgg agaggagttt ggcttcatcc tgcgctatgg
     2101 ccgatatgcg cccaagggca ggaattctca gtttctcatc ggggggctcg gtctggccga
     2161 cctctcgagg cggttgaccg cctggttgga aacgaacggc cccctcgagg gtcaggttca
     2221 agtgatcgat gagggctcgg gcgaactgat ggtttatctg cttcttccgg aggcggcgtg
     2281 acggccgagc cttctctgag gagcatgctg cggtgatggc cggattgaac cgggcgatac
     2341 gttggctcaa gaacctcggg ctctggcgaa cgggaggcgc ggtggcctgc ctgatgctgc
     2401 tggcggctat cttggctttc cccagcgtcg tgctgcctgt ggtcgcgcta ctctgcggcg
     2461 ggctcttttt cttctacgga gtgaagtcgt acgccagcgt cgccatcatc atgctctcca
     2521 cttcgggacc cactggcaat gggaagccga atggcgatgg tcaaaagggc cgccggtccg
     2581 ggctgctcgg gcgtctgaag ggcgggggcc gcgcccatcc cagccggggt agcggaggtg
     2641 gcaagcctta tcggctcccc cctgagaagc agcccttcat ttcgatccag ctcccgatgt
     2701 acaacgagaa ccgggtcgtc gatcggctgc tggcggcctg cacccaactg gactaccaga
     2761 actacgaagt gctcgtcgcg gatgattcta gcgatgagac tctcaagact ctcgaacgct
     2821 gggccaaaca tccgaaggta aaggtctcgc accggatcaa tcggagcggc ttcaagggcg
     2881 c
//
LOCUS       DATDPQ010000094         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1344060, whole genome shotgun sequence.
ACCESSION   DATDPQ010000094 DATDPQ010000000
VERSION     DATDPQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344060"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VI520_02140"
     CDS             complement(<1..471)
                     /locus_tag="VI520_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HLF36713.1"
                     /translation="MSRRTALLIVTTSNGDEAFAQLAPPEIDLAALSASLGNPSMGGF
                     ELTQLVDKDVQTLREAIVRLYRSAGPQDVALLYYAGHAIQDQFGEMYLTARNTQIDVL
                     EATGVQAAFLRDQLDKSRSDQKVVLLDCPTIAAFDGARPLVGSSSGILESIEGSR"
     gene            complement(552..>2282)
                     /locus_tag="VI520_02145"
     CDS             complement(552..>2282)
                     /locus_tag="VI520_02145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLF36714.1"
                     /translation="HSLPLDTVVRLTGQVSSALDYAHRRGIVHRDVKPANIILRAGST
                     PINSQLPLGPDVEPVLTDFGVARIASSTTPTVSGTILGTPAYMSPEQVRGEAVDNRSD
                     IYSLGIILYEVLAGRLPFDPETDTPASILYKHVHENPPQLPNISLAIQAVLKKSLAKD
                     REQRYQQAGQMAADLQASVKAIPSATATAALVTQLSQSSPVVSPPLPRKRSGLPVLAR
                     VGALVVAAIVLVVGAVLGNRLLGGGAQAEQTAPVQESATQLPAESTEPAGAELGDNPA
                     ATELPSDTALAGFAIVRDSILELRLTPVEAPPDGASYHAWLLGAEGTTPLHLNLEGTV
                     ELLAGELQVSFVHPQGANLLADYGTLVISIETEGSVLSEPSSITYRGSVSPDIVGFVR
                     LANEVRSDQRIPTDLVDLFTRQAGHFLSHAGLALGAMGSDNLPDTKTHGEHSLNIIEG
                     ESGEFYEDWNGNGRPENPADDVGLLPYVRLLEATGTGAAISEEQRGGSGEPGRALAAQ
                     AAEIASLLLEAREILRQIVQVDTSADIRSFGLDTELAAVLSVKDLIDQAAAQAADLEL
                     AFAIELFPAP"
BASE COUNT          428 a          697 c          725 g          432 t
ORIGIN      
        1 acggcttcct tcgattgact ccaagatccc tgaactgctg cccaccagtg ggcgcgctcc
       61 gtcgaatgcc gcgatcgtcg ggcagtcgag cagcacgacc ttctggtcgg agcgagactt
      121 gtcgagctga tcgcgcagga aagcggcttg cacgccggtc gcttcgagca cgtcgatttg
      181 cgtgttgcgg gccgtcaggt acatctcgcc gaactgatct tgaatggcgt ggcccgcgta
      241 atacagcaac gccacatctt gcggcccagc tgaccgatac agccgaacaa tcgcctcgcg
      301 cagagtttga acatctttgt ccaccaactg ggtcagctcg aagccaccca tcgagggatt
      361 gccgagcgag gcagagaggg cggccaagtc aatctcgggc ggcgccagct gagcgaaggc
      421 ctcgtcgccg ttcgaagtcg tcacaatcaa gagtgcggtt cgtcgggaca tgtcgggtta
      481 gcttctccgg ggcaagagag attctagacc cgtaagatgc gaattgtaaa tagaaggggg
      541 gcggccaggg actatggtgc ggggaatagc tcgatcgcaa aagccagctc caagtcggcg
      601 gcttgggctg cggcttggtc aatgaggtct tttacgctga gaacggcggc cagctctgtg
      661 tccaggccaa atgagcggat atctgcggaa gtgtctactt ggacgatctg acgcaagatc
      721 tcgcgcgcct cgagcagcag gctggcgatc tccgccgcct gagcggccag cgcccgcccc
      781 ggctcgccgc tcccaccccg ctgttcctct gaaatggccg cgccggtccc tgtggcctcc
      841 agcagccgga cgtacggcag caatccaacg tcatcggcag gattctccgg ccggccgttg
      901 ccgttccaat cctcatagaa ctctcccgac tctccctcaa taatgttgag gctatgctcg
      961 ccatgggtct tggtatcggg cagattgtcc gaacccatgg cgccgagagc caggccggca
     1021 tggctcagga agtgaccagc ctgccgcgta aacaggtcga caagatccgt tgggatccgc
     1081 tgatcgctgc ggacctcatt cgccagccga acaaatccta cgatatccgg cgacaccgac
     1141 ccacgatagg tgattgagct gggctcggac agcacgctgc cctctgtctc aatgctgatc
     1201 acgagcgtcc cgtagtccgc aagcaagttt gcaccttggg ggtggacgaa gctcacctgc
     1261 agttcgccgg cgagcaattc gacagtcccc tctagattga gatgcagcgg cgtggtcccc
     1321 tccgcgccca gaagccaagc gtgataggag gccccatccg gaggcgcctc gaccggtgtc
     1381 aggcgcaatt ccaggatgct gtcccgcacg atggcgaacc cggccaaggc agtatcgctt
     1441 gggagttctg tcgccgcagg gttatcgccc agctcggcac ccgcgggttc ggtgctctcc
     1501 gctggcagct gggttgcgga ctcttgaacc ggggcggtct gctccgcctg tgcaccgccc
     1561 cccaagagtc gattcccgag cacggcgccg acgacgagaa ctatcgcggc cacgacaagc
     1621 gccccaacgc gtgcgagtac tggaaggccg gagcgcttcc tcggtagggg cggggagacg
     1681 actggactcg actgactcag ctgggttacc agggctgctg tggcagtggc tgacgggatc
     1741 gcctttacgg aggcttgcag gtcggccgcc atctggcccg cctgctggta tcgctgttcc
     1801 ctgtccttag cgagcgactt cttgagcacg gcctgaatcg caagactgat attggggagc
     1861 tgaggcggat tctcgtgaac atgcttgtac aggatggagg ctggggtgtc cgtctcagga
     1921 tcgaagggca gtcttccggc caggacctcg tacaggatga tcccaagaga atagatatcc
     1981 gaacggttgt ccaccgcttc gccgcgcacc tgctcggggc tcatgtaagc cggggtgcca
     2041 agaatcgttc ccgaaacggt tggggtcgtc gagctggcta tcctcgcaac cccgaagtcg
     2101 gtcagcaccg gctcgacgtc tggcccaagc gggagctggg aattgatcgg cgtggatccg
     2161 gcccgaagga ttatgttggc cggcttgacg tcacgatgaa cgatgccccg ccggtgagca
     2221 taatccaggg cgcttgatac ttgcccggtt agcctgacga ctgtgtcgag cgggaggctg
     2281 tg
//
LOCUS       DATDPQ010000095         2994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_35970, whole genome shotgun sequence.
ACCESSION   DATDPQ010000095 DATDPQ010000000
VERSION     DATDPQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35970"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1174)
                     /locus_tag="VI520_02150"
     CDS             complement(272..1174)
                     /locus_tag="VI520_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36715.1"
                     /translation="MPGRQPPRVVIVTRSTEYERLIEHHATAGQARFFLRQRSLGTTE
                     VERGHGRVSGSLLAVEQAIPTAWRRNQVQRAELDRFLFEPDDIVVAVGQDGLVANVSK
                     YLQGQPVIGINPDPGRNPGVLVRHAPTAIGGLLARLVQGKAQVEERVMVEARLDDGQT
                     LRALNEVFIGHRSHQSARYRLLWQGQEERQSSSGLIVTTGTGATGWASSILRERGSQL
                     RLPAPTEQRLAFFVREAWPSPATGTGLTEGSIQAGDTLEIRSEMDEGGVAFGDGIEAD
                     SLQLLWGQRVQVSVAESQMRLVVG"
     gene            complement(1176..2198)
                     /locus_tag="VI520_02155"
     CDS             complement(1176..2198)
                     /locus_tag="VI520_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLF36716.1"
                     /translation="MAHIQRFFFLRHLRSEPTSHVLRFRRGRLIRSGRGLAFWFRPLA
                     ATIAEVPVDDREQAFLFTGRSEDFQEVAVQGTITYRVVDPETLASRIDFGIDPAKGFH
                     NRQPLEQLAGLVTQLAQQFALDFVVHTPLRTVLQDGIAKIRDRIIQGMEASEELTQMG
                     IEMVTVRLASVAPSSEVEKALQTPTREAIQQQADQATFARRALAVEKERAIAENELQN
                     RIELARREQALIEQEGLNERRRASEHAEAQRLEAEAEAERTRLSSGAEADTIRLVEAA
                     RNQAEREKMEVYSKLPAGVLLGVAAQTLAGKLQSIEHLNLSPDSLAPLLSSLMSAGTR
                     KLEDGS"
     gene            2320..>2994
                     /locus_tag="VI520_02160"
     CDS             2320..>2994
                     /locus_tag="VI520_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLF36717.1"
                     /translation="MKNKPRGLKRGAVKLDFDLYRVAVPIPGYPEAQLSAVDLQPEGV
                     ERTVVFVHGYAGVAETWDYQLDYFAGSYRVIAPDLRGHGLSDAPDSAYTMPELVSDLV
                     QIADVLKLPERFVLAGHSFGGAVCVEFAVAHPERLEKLILLSTAGEHPLPKLTGLITR
                     MPTAFFRPFWRYRPRWNAEVHVMKRMMVNNLRIWNGWEAMARIKTPTLVITGERDRYF
                     PRRVFDR"
BASE COUNT          498 a          954 c          954 g          588 t
ORIGIN      
        1 ggtctgacct ttctggtgcc ggtggccttt gcggtgacgg tgccggccga ggcgctcacc
       61 gcccgcctga ctccgctcta tctggcgttg gcgctgggga tggccgccct ggccctggtc
      121 gccgcccgct ggttctggca cgtcggcctg cgccattact ccggcgcgtc ggcgtgacag
      181 ggagaaaggg tcatccatcg ccttgtattc ctaggtaggc gagtccggtt ttgctctccc
      241 tgttcgttcc ggagggttgc ctaggggtcc ttcatcccac tacgagccgc atctgcgatt
      301 cggcgacact cacttgcaca cgctggcccc agagcagttg gagcgagtcg gcctcaattc
      361 cgtcgccaaa ggcgacccca ccctcgtcca tttcgctgcg aatttccaga gtatccccgg
      421 cctggattga tccctccgtc aacccagttc cagtggcggg cgagggccag gcctcgcgca
      481 caaagaatgc cagccgctgc tcggtcggtg ccgggaggcg aagctgcgaa ccgcgttcgc
      541 gcaggattga gcttgcccat ccggtggcac cggttccggt ggtcacgatg agacctgagg
      601 aggactgccg ttcttcctgt ccttgccaca gcaggcgata gcgggcgctc tggtgactgc
      661 ggtgtccgat gaacacctcg ttgagcgccc gcagagtctg cccgtcatcc aggcgagctt
      721 ccaccatcac ccgctcctcc acctgggcct tgccctgaac gagccgggcc agcaatccac
      781 caatcgcggt cggcgcgtga cgcactagca ctcccgggtt ccggccgggg tcagggttaa
      841 tcccaattac tggttggccc tgcaggtatt tggatacatt ggcgactagg ccgtcctgcc
      901 cgacagcgac gacgatgtcg tcgggttcaa acaggaatcg gtctagctca gcccgctgca
      961 cctggttcct acgccaggcg gtggggatcg cctgttcgac ggcgagcagt gagccagaga
     1021 ctcggccgtg accccgttca acttcggtcg tgcccagtga gcgttggcgc agaaagaatc
     1081 gggcctggcc agcggtggcg tgatgctcga tgagcctctc atactccgtg gagcgcgtca
     1141 cgatcacaac ccgcggtggc tggcgcccgg gcatcttaag aaccgtcctc gagtttgcga
     1201 gtgccggcgc tcatcaagct ggacaacagc ggcgctagag agtccggtga gaggttgagg
     1261 tgttcgatgg attgcagctt tcccgcgagg gtctgggcgg ccacacccag gagaactccc
     1321 gcgggcagct tgctgtagac ttccatcttc tcgcgctcgg cctggttgcg tgcagcttcc
     1381 accagccgga tcgtgtcggc ctccgcccca gagcttagcc gcgtgcgttc ggcctccgcc
     1441 tcggcctcta gccgctgcgc ctcggcgtgc tcggaggccc tccggcgctc attcagacct
     1501 tcttgttcaa taagcgcctg ctcgcggcgg gctaactcaa tccgattctg cagctcgttc
     1561 tcggcgatag cccgttcctt ttccacggcc agggcgcgtc gggcgaacgt ggcctgatcg
     1621 gcctgctgct gaatggcttc gcgggtgggt gtctgtagcg ccttctcgac ctcggagcta
     1681 ggtgcaacgc tcgccaagcg caccgtgacc atttcaatcc ccatttgagt caactcctcg
     1741 ctggcttcca ttccctggat gatgcggtcc cggatctttg cgattccgtc ctggagcaca
     1801 gtccgcagtg gggtatggac gacgaagtcg agggcaaact gctgcgcaag ctgggtcacc
     1861 agaccagcga gctgttcgag aggctgacgg ttatggaatc ccttcgccgg gtcgataccg
     1921 aagtcgatcc ggctagccag agtctcaggg tccacgacgc gataggttat cgttccctga
     1981 acggccacct cctggaaatc ctcgctgcgg ccggtgaaga ggaaggcctg ctcgcggtcg
     2041 tcaaccggca cctccgcgat cgtggccgcc agcgggcgga accagaatgc cagccctcga
     2101 ccgctgcgga taagccggcc gcgacggaag cggagcacat gcgaggtggg ttcggagcga
     2161 aggtggcgga gaaagaagaa gcgctgaatg tgggccatga tttccctcct tgggatgact
     2221 aacgcgagga cagtgggaat ccttcatgcc ttctggcttt aagtcaagga ttgtgccgag
     2281 acgggataga atcgtgggga tggatcccca gggttccgta tgaagaataa accgcgtggg
     2341 ctcaagcggg gagcggtcaa gctggatttc gacctgtacc gggtggcagt ccccattccc
     2401 ggctaccccg aggcccagct aagtgccgtc gacctgcagc cggagggagt ggaacggacc
     2461 gttgtcttcg tgcatggcta cgccggtgtg gcggaaacct gggactatca gctcgactac
     2521 tttgccggca gttaccgggt gatcgccccc gatctaaggg ggcatgggct gagcgacgcg
     2581 cccgacagcg cctacacgat gcccgagctg gtctccgacc tggtccagat cgccgacgtg
     2641 ctgaagctgc cggagcgttt cgtgctggcc gggcattcct tcggaggggc cgtctgcgtc
     2701 gagtttgctg tggcccatcc agaacggctc gagaagctga tcctactctc gaccgccggc
     2761 gagcacccct tgccaaagct gaccggactg atcacccgta tgccgacagc cttcttccgg
     2821 cccttctggc gctatcgccc gcgctggaat gccgaggtcc atgtgatgaa gcgcatgatg
     2881 gtcaacaacc tgcgcatctg gaacgggtgg gaggcgatgg cccgaatcaa gaccccgacc
     2941 ctggtgatca ccggcgagcg agaccgctac ttcccgcggc gagtattcga ccgc
//
LOCUS       DATDPQ010000096         6431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3696368, whole genome shotgun sequence.
ACCESSION   DATDPQ010000096 DATDPQ010000000
VERSION     DATDPQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696368"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VI520_02165"
     CDS             <1..247
                     /locus_tag="VI520_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36718.1"
                     /translation="VSVETGLVVYELVQINWVDGTGQFWDRLHFAAGVHRSDGSQVRG
                     TVLMQLLADRRLLVEVFMGKRAPQVIGFTNAAVGYER"
     gene            338..1033
                     /locus_tag="VI520_02170"
     CDS             338..1033
                     /locus_tag="VI520_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36719.1"
                     /translation="MRNVTKTYPTPGGALVALSNVSLQVQRGELVVIMGKSGSGKSTL
                     LNMVSGIDRATSGEIFVNGVAIHALSEKELTAWRGRNLGIVFQFFQLMPTLTLVENVM
                     LPMELNRTYSPIDQRRRAVRLCEMVEMAEHRERFPSLVSGGEQQRVAIARALANDPPL
                     ILADEPTGNLDSTTAATIFRLFDELVTNGKSLILLTHDQNMARRAHRALRIVDGRIMN
                     ENGEGRTEVVEVS"
     gene            1044..3401
                     /locus_tag="VI520_02175"
     CDS             1044..3401
                     /locus_tag="VI520_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36720.1"
                     /translation="MGVLWKKILRDLWLNRLRTLLIVVVVVSGTAAFGMLLASRVVTE
                     RTEREQYLGTSPAHAILTIPEFGDALIRRVERVAGVARAEGGRVMQARLQTASGQWIP
                     LEISSVADFEDWQINRVYPENGVVATPAQGAILLERTVLSAGPIGTTVIVQSVRGITE
                     SLEVAGFVNDPSVVQYPLTQSAFGYVSSGTMEALDRSARHNRLYVLTEARQRDSIERI
                     LTAVQDTVEAEGYQLLRAEIPTPGRSLRADPLSAILFVLSQIGLLTLCLAGLLVGNVM
                     ASVMGSEVRQIGILKSLGAGTGRIAGLYARQAAIYGLIAIVVAVPLAHFLGAQLSGVI
                     AERTNFRLPRFWLPSGVLTLQLAAGLGLPLAAASLPVLNGSRKTALRALAEIPPIHWK
                     AGPIGQALARVAGMPGLARMAIRNAFRRTGRMLLTLGMLTLAGAMFVAVLGARRSLQA
                     DLAKIQATVSYDVSLTMVEPLSQKTLLSRVQRIPGIVAVEAWLQSEARIVLADRITGG
                     VPVLGVPSDSTMTRPNIIQGRWFVPDDDFAVFLSSNVLELVPGLKAGDVVVLKIADEE
                     MDWTVIGLEDRQLRPTAIVPLETLERVTGLAGQAQMVVVRTVNSSPSFQETIEADLRE
                     AFRGSSIAGTHTTAEERQSQAAQLDLLLWLLMMVVLLVLVDGGLGLAVTMGLNVMERT
                     REMGVLRALGAQDGSIRGLVISEGLFIGLVSWMLGAAGAVPFGVWLTGVLGTILYGRP
                     VEYIFSVPATLLWLSIVLAIVTVASLLPAQRALDVPVREALGYLG"
     gene            3465..4565
                     /locus_tag="VI520_02180"
     CDS             3465..4565
                     /locus_tag="VI520_02180"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF36721.1"
                     /translation="MDSGSGHLAAAEAIAAGLERLYSGAVHTRIMDLSKEVGSGPLRK
                     SHEAYRWLAGNHPRLYDRLWHSDWFVSGPEFVAVLCRLLAGRTLAKVYQSERPDGVVS
                     VHGLLNHFPQRLLRTALPGAIPFVTVVTDMLTFHRLWINPAVDYCVVPTEQARELAVR
                     YGMAPERVEVLGQPVHPRFAEIRVGKEAGRRDLGLEPHRHCVLITGGGDGIGRLYETA
                     RAIASRVPGVQLILVAARNRALQQQLLSTEWEVPAKVYGFVDNMPELMAASDLVVTKA
                     GPGTLAEAFTVGLPIIVSGYIPGQESANARYVTENMAGAFLQEPDEIASTIREWIADS
                     SRLNRMSQNAGKLARPDAALRIAERLFQLVEP"
     gene            4574..5179
                     /gene="plsY"
                     /locus_tag="VI520_02185"
     CDS             4574..5179
                     /gene="plsY"
                     /locus_tag="VI520_02185"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLF36722.1"
                     /translation="MWVVAAYFLGATPTGYLAGRLGSGIDLREHGSQSLGGTNVYRLL
                     GWKYAIPVALIDLAKGAIPVLFGRSLAEGGVAQAWVPAALGAAAVLGHMYSPYVRFRG
                     GKGMATATGMFLVLAPLAILLAAPVWGAVMWFTGYVSASSIVTTALFPLWVRLSVPEA
                     PYPFWASVGLATLIIFAHRSNVRRIISGTENRFRTRHRRVG"
     gene            5605..5805
                     /locus_tag="VI520_02190"
     CDS             5605..5805
                     /locus_tag="VI520_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36723.1"
                     /translation="MSVGVVIADADGADSWGVDYYGQVNRVFWLPSGERVGIAGGICA
                     RVGVVPVVAGWGTPCLRSQWRE"
     gene            6078..>6431
                     /locus_tag="VI520_02195"
     CDS             6078..>6431
                     /locus_tag="VI520_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36724.1"
                     /translation="MKTHKWPFAAALSAIILAVLACGGSFSTANIKSAWLSADSSGTP
                     ETTQFTQDQQTIYCIVELRSAPDDTIVKAVWTAVSAEGSDPNLLIDETEFTSGDATLT
                     FDLTNDLLWPVGSYKV"
BASE COUNT         1109 a         1953 c         2113 g         1256 t
ORIGIN      
        1 ggtcagcgtc gagaccggcc tggtcgtgta cgaactggtt cagataaact gggtcgatgg
       61 aaccggccag ttctgggacc gactccactt tgcggccggc gtgcatcgga gcgacggttc
      121 gcaggtgcgg gggacggtgc tgatgcagtt gctcgccgac cgacgtctcc tggtcgaggt
      181 gttcatgggg aagcgcgcgc cccaagtgat aggtttcacg aatgccgccg ttggctatga
      241 gcggtgaagc cagatagtag cgcggcctgt ctctcacatt ccgaggtaga cctggtcgag
      301 gccgatcgcg atgagagatg atccgccaat tcttgacttg cggaatgtaa cgaagactta
      361 cccaaccccc ggaggcgcgc tggttgcgct cagcaatgtc agtctccagg ttcagcgcgg
      421 tgagctcgta gtgatcatgg gcaagtctgg cagcgggaaa tcaactctgc tcaacatggt
      481 cagcggtatc gatcgggcca ccagcggaga gattttcgtg aacggggtcg cgatccatgc
      541 cctttcggag aaggagctca cggcatggcg aggccggaac ctggggatcg tcttccaatt
      601 cttccagctc atgccaaccc tgacgctggt ggagaatgtc atgctaccca tggagctcaa
      661 ccgaacgtac tcgccgatcg atcagcgccg gcgcgccgtt cggctgtgcg agatggtgga
      721 gatggccgag caccgggaga gatttcccag cttggtttct ggaggcgagc agcagcgtgt
      781 cgcgatcgcc cgcgccctgg ccaacgatcc cccgctgatc ctggccgacg aaccaaccgg
      841 gaacctggac tcaaccacgg ctgcaacaat attccggctg tttgacgagc tcgttacgaa
      901 cggtaagagc ctaatcctgc tcacccacga tcagaatatg gcccggcgcg cccaccgcgc
      961 cctccgaatc gtggatggac ggatcatgaa tgagaacggg gaaggtcgca ccgaggtcgt
     1021 agaagtgtcc tgaggagtgg ttagtgggag ttctctggaa gaagatcctg cgcgacttgt
     1081 ggctcaaccg gctgcggacc ctgttgattg tcgtggtggt cgtttcgggc accgccgcat
     1141 ttggcatgct gctcgccagt cgggtggtca ctgaacgtac cgagcgagaa cagtacctgg
     1201 gcacctcacc ggcccatgcg atcttgacca tccccgaatt tggcgacgcg ctgatccgga
     1261 gggttgagcg ggtggctggg gtggcgcggg cggaaggcgg gcgagtcatg caggcgcggc
     1321 tgcagaccgc gtcgggccag tggattccgc ttgaaatcag ctccgtggca gatttcgagg
     1381 actggcaaat caaccgggtc tacccggaga atggcgtcgt tgccactccg gcccaggggg
     1441 ccatcctgct ggaacgcacc gtcctcagcg caggtccgat aggcactacc gtcatagtgc
     1501 agtccgtgcg ggggataact gagtccctcg aggtggcggg gtttgtgaac gacccgagcg
     1561 tagtgcaata tccgctgacc cagtcggcct ttgggtacgt cagctcaggc acaatggagg
     1621 ccctcgaccg gtccgcccgc cataaccgtc tgtacgttct gaccgaggcg aggcaacggg
     1681 actccatcga gcgcatccta accgccgtgc aagacacggt ggaggcggag ggctaccagc
     1741 ttctgcgggc ggagatcccg actccaggca ggtcgctgag ggccgatccg ctttccgcga
     1801 tcctattcgt cctcagccag attggattgc tgactctctg cctggcgggc ctgctggttg
     1861 gaaacgtcat ggcctcggta atgggcagtg aggtgcgtca aattggcata ttgaagagcc
     1921 tgggagcggg tacgggccgg atcgcgggcc tctatgcacg tcaggcagcg atttatggcc
     1981 tcatcgccat tgtggtcgcc gttcctttgg ctcattttct gggcgcgcag ctctcgggag
     2041 tgatcgcgga gcggaccaac ttccggctgc cccgcttctg gctgccctcc ggagtattga
     2101 cgctgcagct ggccgccggg ctgggcctgc cgttggccgc ggcctccctc ccggtcctca
     2161 acggatcgcg caagaccgct ctcagggcgc tggctgaaat ccccccgatt cactggaaag
     2221 ccggaccgat cggccaggcc ctcgcgcgag tggctggcat gcccgggctt gcacggatgg
     2281 ccattcgaaa tgcttttcga cgcaccggcc ggatgttgtt gacgctgggg atgctcacgc
     2341 tggcaggagc gatgttcgtc gccgtcctgg gagcccggcg gtcgctgcaa gcagacctgg
     2401 cgaagatcca agcaaccgta agttatgacg tctcgctcac catggtcgag ccgctttctc
     2461 agaaaacgct cctcagccgc gtccagcgca ttcctgggat tgtagcggtc gaggcctggc
     2521 tgcaatccga agcccgaatc gtcttggctg atcggatcac cgggggcgtg ccggtccttg
     2581 gagtgccaag cgattcgacg atgacgcgtc caaacatcat ccaaggacgc tggtttgttc
     2641 cggatgacga ctttgccgtc tttcttagca gcaacgtcct ggaactcgtc cccggcctga
     2701 aggccgggga cgtggtcgtt ctcaagatcg ccgatgagga aatggactgg acggtgatcg
     2761 gtctcgagga ccgacagctc cggcccaccg ccattgttcc gctcgagacg ctcgagcggg
     2821 tcactggcct ggcaggccag gcccagatgg tggtcgtgcg cactgtaaat agcagcccga
     2881 gctttcagga aacgatcgag gctgacctgc gagaagcatt ccgaggctcg agcattgccg
     2941 gcacccacac gaccgccgaa gagcggcaat cgcaggcggc tcaactggat ctgctcttat
     3001 ggctgctgat gatggtcgtc ttgctcgtgc tggtcgacgg cggccttggg ttggcggtga
     3061 cgatgggtct caatgtcatg gaacgaaccc gtgagatggg ggtcctgcgg gcattgggcg
     3121 cacaggatgg ctccattcgg ggtcttgtaa tctcggaggg gctcttcatt ggtttggtga
     3181 gctggatgct gggcgccgct ggggccgttc cttttggtgt ctggctgacg ggggtgcttg
     3241 ggaccatcct ctacggccgg ccggttgagt acattttctc cgtcccagcg acgctgctct
     3301 ggctgtcgat cgtgcttgcc attgtcacgg tagcgagcct acttcccgcg cagcgggcgt
     3361 tggacgttcc ggtacgagag gcattgggtt accttggctg agatgtcccc gggtgagttt
     3421 tccccagtcg gtgccgagcg acgaccggca tcctgatcct cacgctggat tcgggcagcg
     3481 ggcacctcgc ggccgccgag gcgatcgcgg caggcctcga gcggctatac agcggcgcgg
     3541 tccatacccg cattatggat ctctccaagg aggtcggatc cgggccgctc cgaaaatcgc
     3601 acgaagccta ccggtggctc gcggggaacc acccccggtt gtatgaccga ctctggcata
     3661 gcgattggtt cgtgtctggt cccgagtttg ttgccgtgct ctgccggctg ctggcgggcc
     3721 gaaccctggc gaaggtctac caatcggagc ggcccgatgg ggtggtctcg gtgcatgggc
     3781 tgctcaacca cttcccgcag agactgctgc gcacggcgct ccccggcgcg attccgttcg
     3841 tgactgtcgt gacggatatg ctcaccttcc atcgcctgtg gatcaatccc gcagtcgact
     3901 actgtgtcgt cccaacggag caagctcgcg agctcgccgt gcgctacgga atggcgcctg
     3961 agcgggtgga ggtcttgggc cagccggtac atcctcggtt tgccgagatc agggtgggca
     4021 aggaagctgg acggcgagac ctggggctgg agccccatcg tcactgcgta ttgatcaccg
     4081 gcggcggcga cgggatcggc cgcctgtatg aaaccgcgcg ggcgatcgcc agccgggttc
     4141 ctggcgttca gcttatcctc gtggcagcta gaaaccgggc gctgcagcag cagctgcttt
     4201 caacggagtg ggaggtcccg gcgaaggtct atggctttgt cgacaacatg cccgaactga
     4261 tggccgccag cgacctggtc gtgacgaagg ccggccccgg gacgctggca gaggcgttta
     4321 cggtcggtct gcccatcatc gtgtcgggct acataccggg ccaagaatcc gcgaatgccc
     4381 ggtatgtaac agaaaacatg gccggcgcct tcttgcagga gccggacgag atcgcgagca
     4441 caatccggga atggatcgcg gactcgagtc gattgaatcg gatgtcgcaa aacgctggca
     4501 agctggcccg gcccgatgcc gccctccgga ttgcagagcg cctctttcag ctggtggaac
     4561 catgagcgaa ctggtgtggg tagtcgcagc ttacttcctg ggcgccactc cgactggcta
     4621 cctggcgggc cggctcggca gcggtatcga cctgcgggag cacggcagcc agagcctggg
     4681 cgggaccaac gtctaccgcc tgctcggctg gaaatacgcg atcccggtag ccctgatcga
     4741 tctcgcgaag ggcgcgattc ccgtgctgtt cggccgttcg ctcgcggaag gcggcgtggc
     4801 tcaggcatgg gtgcctgcgg cgctcggggc cgcggcggtg ctcgggcaca tgtactcccc
     4861 atacgtacgg ttccgcggcg ggaagggtat ggcgaccgcc acgggaatgt tcctggtcct
     4921 cgcgccccta gccatcctgc tcgccgcgcc ggtgtggggc gcggtgatgt ggtttaccgg
     4981 ttacgtgtcg gccagctcga tcgtgaccac cgcgctcttc cccctgtggg tgcggctcag
     5041 cgtgccggag gcgccgtacc cattctgggc aagcgtcggc ctggccacgt tgatcatttt
     5101 cgcccaccgc tccaatgtgc ggcgcatcat ctccggcacc gagaaccgtt tccgcacccg
     5161 ccaccggcga gtcgggtaaa acgtagtgcg cctggccgta atctcactgg tcctgatcct
     5221 gggtatcccg gcctgcagct tatccgccgc gcccgacgag ttccctcgca gccagaacac
     5281 gcagactgca gaggccggca atcgccgggc ccaccagcat cctcttcgtc tccgtccgag
     5341 atggagtggc cgacctctac cggatggacg aagatggcaa gggcgacgtc cgcctgaccc
     5401 agaatggtcg caacaataag tggcccagct ggtcgccgga cggtcaacag atcgcgttca
     5461 tcagcagcga agttggcgcg gatccggata catcggcgac ccagttgctc gtgatgggtg
     5521 ccgacggaac cgggctcggt aggctgctgg tcgcccacct ggtctcccga tggttcgaag
     5581 ctggcctaca tctgtcgagc cctaatgagt gtcggggtcg tgattgccga cgccgatggg
     5641 gccgattcat ggggcgtgga ttattacggg caggtcaacc gagtcttttg gctgccgtcc
     5701 ggagagcgcg tggggattgc aggcggtatc tgtgcgcggg tgggggtagt tcccgtggtc
     5761 gccggatggg ggacgccttg ccttcgcagc caatggaggg agtgacaaag aaatcttcgt
     5821 cttcgatctc gccagccagt ctgtcgtcca gctgaccgac aacgaggtcg acgactacct
     5881 gcccgcttgg cagccctagc ttctcgggtc gcccggcggt ggcggcccgc cgctcacctc
     5941 agcccgcggc gatcgagcgc ggcccgggtc atcggcctgc ggcccgagta ctcggccagt
     6001 ggtgcgagca gcgcaggcca ctagcctcca cgggtatttg cgcgaaactc cccaacccaa
     6061 gccaaaggag gctttgagtg aaaacccaca agtggccatt tgctgctgcc ctttcagcga
     6121 ttatcctggc tgtgctcgcc tgcggtggca gcttcagcac cgccaacata aaaagcgcct
     6181 ggctctccgc cgatagttca ggtacgcccg aaaccaccca gttcacccag gatcagcaga
     6241 cgatttactg cattgttgag ctgcggagtg ctccagacga taccatcgtg aaggctgtgt
     6301 ggaccgccgt cagcgccgag ggaagcgatc ccaatctcct cattgatgag accgaattca
     6361 catccggcga cgccactctg accttcgatc ttacaaatga tctgctgtgg cctgttggta
     6421 gctacaaggt c
//
LOCUS       DATDPQ010000097         4518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2646912, whole genome shotgun sequence.
ACCESSION   DATDPQ010000097 DATDPQ010000000
VERSION     DATDPQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646912"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VI520_02200"
     CDS             complement(<1..348)
                     /locus_tag="VI520_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36725.1"
                     /translation="MEGLQTKPPLVVHSPHPMAYSVDELICVCISRRIKNGDVVALGL
                     ATPIAAVGALLAQKTHAPAIYLASAIGQCFCRTGPRLELQGAEGSWLAHSLGSFGFAQ
                     AAADFLPAAKPLEF"
     gene            complement(404..1741)
                     /locus_tag="VI520_02205"
     CDS             complement(404..1741)
                     /locus_tag="VI520_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36726.1"
                     /translation="MTLPRTSPRVIRTADMPTTGSLRYIPSSVNRIEDTGLNQLWLQD
                     LALKLLYFRGYLTGFKVAEAMALPYAGLSDLVLEALKREKFVEVKSQIGIGEGSYQYA
                     ITDAGIARAREALERSQYAGPAPVPIAVYNEAILRQSRSRTTVNPRTMSKALEGLTLS
                     ETTFNQIGPAVNSGASIFLYGPPGNGKTSIARAIGRLLLLEDITIPYAIDIDGQVVKL
                     YDSVNHELSPDQTSETGGTGALKGDPRWVKIRRPFIVVGGELTMDGLDLVYDDVNKFY
                     EAPFQVKANGGVFLIDDFGRQQVRPRDLLNRWIVPLENRIDFLTLHTGRKVEVPFDVL
                     VVFSTNLPPKDLVDEAFLRRLRHKIEIGDPSYEAYRDIFKRTAEAKGVPYSDRGLAYM
                     LQEWYIKRNRKLRASHPRDICDQILDISRYLNVEAAMSKELIDKAAEAYFVEL"
     gene            1822..2508
                     /locus_tag="VI520_02210"
     CDS             1822..2508
                     /locus_tag="VI520_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF36727.1"
                     /translation="MSLTFLIPVYNERATIEAILRQVKSVNLADEIIVVDDASTDGTR
                     ELLQELIPALPGARLLLHEANRGKGAAIRTGLGLVSSDLVLIQDADLEYDPREVPNLL
                     RPIQDGKADVVYGSRFLGGPRRTSMFWHMVANKLLTLMTNVLYNSILSDMETGYKLFR
                     RELIQSITIRSNRFDFEPEITAKLLKRKARIFEVPISFNPREYSEGKKIGLADAFAAV
                     WALLKYRIVD"
     gene            complement(2505..4178)
                     /gene="recJ"
                     /locus_tag="VI520_02215"
     CDS             complement(2505..4178)
                     /gene="recJ"
                     /locus_tag="VI520_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HLF36728.1"
                     /translation="MLGKRWMEPGTETAWADPGGFTPTEQAILAGRGVRTAVDAERFL
                     SRLHTGSSDPFLMTDMPAAVERLQAARAGGQTVVVYGDYDADGVSATVLLYEVLSSQG
                     FATRWYIPDRFEEGYGLNAGALSKLRSEGADLLVSVDCGIRSWEAAEVAKQVGLELII
                     TDHHLPADELPPAIAVVNPNRQGDRYPFKGLSGVGVALKLAQALLEASGIQYPVLPLD
                     LVAVGTVSDLAPLQNENRALVASGLEQLNAGVRPGLHALVAQARLTPGRLTANSIGFS
                     LGPRLNAAGRLASAGEAVELLLARPGPEARARAESLDRLNRERQRLTNETLDQAREIV
                     GLSPAGRSLIVAADPSFHEGVVGLVAGRLVDEFYRPALVARVEENSVRGSARSIPQFN
                     ITDALDKCGDLLERYGGHAAAAGFGIGAANWDAFVARMERIAEQALGGQDLRPTLSID
                     AVLGFDQLNDDLMDFVEKLEPCGEGNPAPIFATRGATVLSQRAVGADRKHLKLALHSG
                     DRAFDAIGFNLGARLPEISRTVDIAYRLERNEFRGVVSLQLVVVDFRPG"
     gene            complement(4257..>4518)
                     /locus_tag="VI520_02220"
     CDS             complement(4257..>4518)
                     /locus_tag="VI520_02220"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HLF36729.1"
                     /translation="IVPIIILLMADKKDRPFIRAHNAQALAWGVFNLVGGTILSTVLI
                     FCFGAPSLIIWAIGVYWGIKAYQGEQVNIPVLTNFVKGQGWA"
BASE COUNT          886 a         1413 c         1333 g          886 t
ORIGIN      
        1 gaactccagg ggtttggccg ccggcaagaa gtcggctgcc gcctgggcga atccgaagct
       61 ccctaacgag tgcgccagcc agcttccctc ggctccctgc agctccagcc gagggcctgt
      121 ccggcagaag cactgcccaa tcgcggaggc caggtagatc gccggtgcgt gagtcttttg
      181 agcaagcaag gcgcccaccg cagctatcgg ggtggccagc cccagagcaa ccacatcacc
      241 gttcttgatt cgccgcgaga tgcaaacgca gatcagctca tcgaccgagt aggccatcgg
      301 gtgcggtgag tggacgacca gcgggggttt ggtttggagg ccttccatgt tcagtcaacc
      361 tgccccgcgc gctgcggggc agcggggtcg ggtgccggag gtgctacagc tctacgaaat
      421 aggcctcggc ggccttgtcg atcagctcct tgctcatcgc cgcctcgacg ttcaggtagc
      481 gcgagatgtc gaggatttga tcgcagatgt ctctgggatg cgaagcccgc agtttgcgat
      541 tgcgcttgat gtaccactcc tgaagcatgt aggccaggcc tcggtccgag tacggaaccc
      601 ctttggcctc cgcggtccgt ttgaagatgt cccggtaagc ctcgtaggac gggtctccga
      661 tttcaatctt gtgacgtaat cggcgaagga acgcttcatc caccaggtcc ttgggaggca
      721 ggttggtcga gaacacgacc aacacgtcga atgggacctc caccttgcgg ccggtatgca
      781 gagtcaggaa atcgatccgg ttctcgagcg gaacgatcca gcgattgagc aagtcgcgcg
      841 gccgcacctg ttggcgtcca aagtcgtcaa tcaggaagac ccccccattc gccttcactt
      901 gaaagggtgc ctcgtagaat ttgttgacat cgtcgtacac gaggtccaac ccatccatgg
      961 tcagctcgcc accgaccacg atgaacgggc ggcggatctt cacccaacgc gggtcgcctt
     1021 tcaacgcccc ggtgcctccc gtttcggagg tctgatcggg agagagctca tggttgaccg
     1081 agtcgtacaa cttgacgacc tgcccgtcga tatcaatcgc gtatggaatc gtgatgtcct
     1141 ccagcagcag tagccttccg atcgcgcgtg cgatggaagt cttgccgttg ccgggtggtc
     1201 catacaggaa gattgaagcg cccgaattta ccgcagggcc gatctgattg aacgtggtct
     1261 ccgagagggt caggccctcg agcgccttcg acatcgttcg cgggttcacc gtggtccgac
     1321 tccggctttg gcgcaggatc gcctcgttgt acacggcgat cgggaccggg gccggcccgg
     1381 catactgact tcgctccagg gcctcccggg ctctggcgat cccggcgtcg gtaatcgcgt
     1441 actgatagga tccttccccg atcccgatct gggacttgac ctccacgaac ttctcgcgct
     1501 tgagtgcttc caggaccagg tcgctcagac cggcgtaggg gagggccatc gcctcggcaa
     1561 ccttgaaacc ggtgagatag ccacggaagt agagcagctt aagtgccagg tcctgcagcc
     1621 acagctggtt gaggcccgta tcttcaatcc gattgacaga acttggaatg tagcggaggc
     1681 taccggtggt cggcatatcg gcagttcgga tgacacgagg cgaagtgcgg ggaagggtca
     1741 tggccccgat tatatacttc cggccaaagg ggagcaaatc ctcagttgtt cccacccggc
     1801 cggctctgta caatactcca gatgagcctg accttcctga tcccggtcta caacgagcgg
     1861 gccacgatag aggccatcct tcggcaggtc aagagcgtca atctggccga tgaaatcatc
     1921 gtggtcgatg acgcttcgac tgacgggacc cgagagctgc tccaggagct catcccggcc
     1981 ctaccggggg cccgcctgct gctgcatgaa gccaatcggg ggaaaggcgc cgcgattcgg
     2041 accggactcg ggctcgtgtc ttctgatctg gtgctcatcc aggacgccga cctcgaatac
     2101 gatccgcggg aggtccccaa cttgctccgg ccgatccagg acggcaaagc tgacgtcgtc
     2161 tatggctctc gttttctcgg tgggccccgc cggacctcca tgttttggca tatggttgcc
     2221 aacaagctgc tcaccctgat gaccaacgtg ctctacaaca gtatcctgtc ggacatggag
     2281 accggataca aactcttccg ccgagagctg attcagtcga tcacgatccg atccaaccga
     2341 ttcgattttg agccggagat tacggccaag ctgctcaagc gcaaagcacg catcttcgaa
     2401 gtgccgatct ccttcaaccc gcgggaatac tcggaaggca agaaaatcgg tctcgctgac
     2461 gcctttgccg cggtctgggc tctgctgaaa taccggattg tcgattaacc tggcctgaaa
     2521 tccacgacga ccagctgtaa gctgacgaca ccccgaaact catttcgctc caaccggtag
     2581 gcaatatcca ccgtcctgga gatttcaggc agtcgcgccc ctaaattgaa cccaatcgca
     2641 tcgaaggccc gatcgccact gtgcagggcc agcttgagat gcttgcggtc ggcaccgact
     2701 gcacgctggg acaggacggt agcgccccga gtggcgaaga tcggagccgg gtttccttca
     2761 ccgcacggtt cgagtttttc gacgaagtcc atcagatcat cattgagctg gtcaaagccc
     2821 aagaccgcgt cgatcgacag ggttggccgc aagtcctggc cgcctaacgc ctgctctgcg
     2881 attcgttcca tcctcgcaac gaacgcgtcc cagtttgcgg cccctatccc gaatccagcg
     2941 gccgccgcat gtccgccgta ccgttcgagt agatcgccgc atttatcgag ggcgtcggta
     3001 atgttgaact gcgggatgct gcgggccgag cctcgaacgc tgttttcctc tactcgggcc
     3061 accagcgcgg gtcgatagaa ctcgtcaacc agccgcccag ccacaagccc cacgacgcct
     3121 tcatggaaac ttggatcagc tgcgacgatc aagcttctcc ccgccggaga aagcccgaca
     3181 atctccctcg cttgatcaag cgtctcattc gtcagtcgct gtcgttctcg attcaagcgg
     3241 tcgaggcttt cagcccgggc tcgggcttcg ggccctggtc tggcaagcag cagctcgacc
     3301 gcctccccgg cgctggcgag cctcccggct gcattcagcc ttgggcctag actgaaacca
     3361 atggagttag ccgttagccg gccgggggtc agacgggcct gtgcgacgag cgcgtgcaaa
     3421 cctggtcgaa caccggcgtt cagctgctcg agcccggagg cgaccagcgc ccgattttca
     3481 ttttgaagcg gtgcaaggtc ggatacggtt ccgaccgcga ccaggtccag cgggagcacc
     3541 gggtattgga ttccgctggc ttcgagcagc gcctgggcca gcttgagcgc aactccgact
     3601 cccgagagcc ctttgaacgg atagcgatcg ccctggcggt tcggattgac taccgcgatc
     3661 gcagggggca attcgtcagc cggcaggtgg tggtccgtaa tgatcagctc gagcccgacc
     3721 tgcttggcaa cctcagcagc ctcccaggag cggatcccgc agtcaacgct caccagcagg
     3781 tctgctcctt cggagcgcaa tttggaaagc gcgccggcat ttaagccata accttcctca
     3841 aatcgatcag ggatgtacca acgggtggcg aacccttgcg agctcaggac ctcatacagc
     3901 aggaccgtgg cggagacgcc gtccgcgtcg tagtcgccgt agacgaccac tgtttggccg
     3961 ccggcgcggg ccgcctgcag ccgctcgacc gcagccggca tgtccgtcat gagaaatggg
     4021 tcgctcgagc cagtgtggag ccggctgaga aaccgctcag catccaccgc agttcgcacc
     4081 cctctcccag ctaggatcgc ctgttcagtc ggagtgaagc cgcctgggtc tgcccaggcc
     4141 gtctcagtgc ctggctccat ccagcgtttg ccgagcatca aataaggtta tcgcctcggt
     4201 cggcagcaaa acggcccgct ttttgagcgg gccgtctaag tcaattccgg attaggttac
     4261 gcccagcctt ggcccttcac gaaattggtg aggaccggga tgttgacttg ctccccttgg
     4321 taagccttga ttccccagta gactccaatc gcccaaatga tcaaggatgg agcgccgaaa
     4381 cagaaaatca gcaccgtgct gaggatcgtc cccccgacca ggttgaacac cccccacgcc
     4441 agtgcttgag cattgtgggc ccggatgaaa ggacgatctt tcttgtcagc cataagcagg
     4501 atgatgatcg gcacgatt
//
LOCUS       DATDPQ010000098         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3432506, whole genome shotgun sequence.
ACCESSION   DATDPQ010000098 DATDPQ010000000
VERSION     DATDPQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3432506"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VI520_02225"
     CDS             complement(<1..220)
                     /locus_tag="VI520_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36730.1"
                     /translation="MHSPLNHSIRVWLRRGLLLSLGLLLMSLPGPAAAQGKTLYWERF
                     DVTLAIQPNGDLRVVERQRIRFTSGTFTY"
     gene            complement(352..912)
                     /locus_tag="VI520_02230"
     CDS             complement(352..912)
                     /locus_tag="VI520_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36731.1"
                     /translation="MIYAGEVQEREDLSHPIRRQLEAESETRSPAEHDLVLVAILNRP
                     RDLEIARTLGWYRIPLGTAPRTVRVDWLAFYLPSAFGEQRWSVRYLAEVRGIELLTRR
                     ELLYLEADHPRAAHPYYKLQLGPLLELDPPIPSRSWRRFSFLYTTGDRLFTAVDLTQL
                     RVPASRARERLWRMLRERTGFAAVGV"
     gene            complement(936..>2367)
                     /locus_tag="VI520_02235"
     CDS             complement(936..>2367)
                     /locus_tag="VI520_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLF36732.1"
                     /translation="AAQRAKERSIVIPTFEQMRDPSRTPAAIREKLKNVGLWDLDPLN
                     LFRINWKNEPIASGGGFGKVNFLEFPPSLTGVEARILALVGKWFPTGAHKVGAAFACL
                     VPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLGCQSIAILPEGMSKERFEWLAKI
                     AGEVIKTPGSESNVKEIFDKTWELRRSGEDLMIFNQFEEFGNYLWHYNVTGHAMLELL
                     ERELGPREAFRGVTLTTGSAGTLACGDLLKQHFPSSKIAAGEAAQCPTLLENGFGAHR
                     IEGIGDKHVPWIHNVKNTDLVIDLDDAGIVALARLFNEPVGRRYLTGRGVPDHTVEQL
                     DLLGFSGIANLTMAIKFAKYYELGRTDAVLTVLTDSMDLYRSRLDEMRVDYGEYREPQ
                     AAADCARYLHGLTTDGLLELRYTDRRRIHNLKYFTWVEQQGRTHEELVAQWSDSGYWT
                     GVQGQASAIDERIQDFNERVGLLEAV"
BASE COUNT          428 a          753 c          761 g          425 t
ORIGIN      
        1 catatgtgaa agttcctgag gtgaagcgga tccgctgccg ctcgaccact cgcaggtcgc
       61 cgttcggctg aatcgcgagg gtgacatcga accgctccca gtagagcgtt ttgccctggg
      121 cggccgcggg tcccggcagg ctcatcagca gcaggccaag cgagagcagc agaccccggc
      181 gcagccagac ccggatcgaa tgattcagcg gggaatgcac cgggctgatg atacctgcaa
      241 cccggggcga agtcgagccg ccgcgcgtcc tagtgcacgg cagggtgcca ggcgcgccag
      301 gtcaggcccg ggccccggcc atgccggcag gcggacaacc aacgagcgaa ttcagacccc
      361 gactgcagcg aatccggtcc gctcgcggag catccgccac agccgctcac gggcgcggga
      421 agccggaacc cggagctggg tgagatcgac cgcggtgaac agccgatcgc cggtggtgta
      481 gaggaagctg aatcggcgcc agctgcgaga gggtatcggc gggtcgagtt ccagcagcgg
      541 cccaagctgc agcttgtaat aaggatgggc ggcccgcggg tgatcggcct cgaggtacag
      601 cagctcccgg cgggtcaata gctcaatccc ccggacctca gccaggtatc ggaccgacca
      661 tcgttgctca ccgaaggcgg acggcagata gaaggccaac cagtctactc ggacggttct
      721 tggcgcagtc ccgagcggga tccgatacca acccagggtc cgggcgatct cgaggtcccg
      781 aggccggttc agaatcgcga ccaggacgag gtcgtgctcg gccggagatc gggtttcact
      841 ctctgcttcc agttggcgcc ggatggggtg cgaaaggtct tcgcgctcct gcacctctcc
      901 agcatagatc atttgttcta ggttgtcaag gccccttaga ccgcttcgag caatcccacc
      961 ctctcgttga agtcctggat ccgttcgtcg atcgccgagg cctggccctg cactccggtc
     1021 caatagcccg aatcactcca ctgggcgacc aactcctcat gggtccgtcc ttgctgctcc
     1081 acccaggtga agtatttcag attgtggatc cggcgccggt cggtgtagcg caactccaac
     1141 aagccgtcgg tcgtgaggcc gtgcagatag cgggcacaat cggccgcggc ctggggctcg
     1201 cggtactcac cgtagtcgac ccgcatctcg tctagccggc tgcgatacag atccatcgaa
     1261 tcggtcagga cggtaagcac tgcgtcagtc cgaccgagct cgtagtattt ggcgaacttg
     1321 atggccatcg tcaaattggc gatccccgag aaaccgagca gatcgagttg ttcgaccgtg
     1381 tggtcgggga ctccccggcc agtcagatac cggcgtccca ccggctcgtt gaacagccgg
     1441 gcgagagcga cgatcccggc gtcgtccagg tcgatgacca ggtcggtgtt cttcacgttg
     1501 tggatccaag gcacgtgttt gtcaccgatc ccctcgatcc gatgagcgcc aaacccgttc
     1561 tcgagcaggg tggggcactg ggcggcctca ccggctgcga tcttgctgga ggggaagtgc
     1621 tgcttgagca gatcgccgca agccagggtg ccggccgagc cggtcgtcag cgtgacgccc
     1681 cgaaaggcct cgcggggtcc gagttcgcgc tcaagcagct ccagcatcgc atgcccggtc
     1741 acgttgtagt gccagaggta attcccgaac tcttcgaact ggttgaagat catcagatct
     1801 tcacccgagc ggcgcagttc ccaagtcttg tcaaagatct ctttgacatt cgactcgctc
     1861 ccgggagtct tgatcacctc cccggcgatc ttggccagcc actcgaagcg ttctttgctc
     1921 attccctccg gcaggatggc gatcgactgg cagccgagca gggccgaatc gtaggccccg
     1981 ccgcggcagt agttgccggt cgagggccag accgcctttt gggtcgtggg gtcgaactgg
     2041 ccggtcacga gccgcggcac caagcaggcg aacgcggcgc cgactttatg ggcgccggtg
     2101 gggaaccact tcccgaccag ggccaggatc cgggcctcga ccccggtcag actcggcggg
     2161 aattccagga agttgacctt cccgaaccct ccgcccgagg cgatcggctc gttcttccag
     2221 ttgatgcgga acaggttgag tggatcgagg tcccacaggc cgacgttctt taacttctcc
     2281 ctaatcgcgg ctggggtccg gctcggatcg cgcatctgct cgaaggttgg gatgacgatg
     2341 cttcgctctt tggcccgctg ggcggca
//
LOCUS       DATDPQ010000099         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1346054, whole genome shotgun sequence.
ACCESSION   DATDPQ010000099 DATDPQ010000000
VERSION     DATDPQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346054"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VI520_02240"
     CDS             <1..306
                     /locus_tag="VI520_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF36733.1"
                     /translation="AVIGLMKSLANELGPKGIRVNSVNPAWTWTDRIEKLMTDRAQAN
                     KTTVEEEAAKAAAGLPLGRMGTLEEYGKTIAWLASPAAGFVHGHALMFDGGGVSAPI"
     gene            313..1236
                     /locus_tag="VI520_02245"
     CDS             313..1236
                     /locus_tag="VI520_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLF36734.1"
                     /translation="MRIAIFGTGGVGGYFGGLLAHSGHEVTFIARGEHLRAIRAAGLK
                     VKSVNGDFSVHPAQATDAPADIGPMAYVVVAVKQYDLREAASQIRPLIGPETTVVPLL
                     NGVDAHELLIEAVGTGPVVSGLCSLVSLVEAPGVIRQESKLRRVVVGELDRRKSDRVE
                     RLVGAWAAAGAEAIHAEDILVALWTKFVFIASFGGVTALARCTAGELLGSCETRELFI
                     QAMREVEAVARARHIELAPDAVERGLSIAVDFEPTATSSMQRDVAAGKPFELEAFSGT
                     VVQLGVEVGVPTPVHRMIYGLLKPALLKAMG"
     gene            complement(1336..>3045)
                     /locus_tag="VI520_02250"
     CDS             complement(1336..>3045)
                     /locus_tag="VI520_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36735.1"
                     /translation="TDQDLGGLLPADLDGPIPPAGAPNPFVLVDDDGWGYSPDQLQIW
                     NFHADWGDPFSSTFMLDRTLATAAFDSNLCEYARNCIPQPGGTSLDSIPDRLMYRLQY
                     RNFGSHQSLVANHTVDVNGADQAGIRWYEVRDSGGGWSIFQQGTYSPDATHRWMGSAA
                     MDGLGNLALGFSASSTSVHPSIRYAGRLAGDPTGTLAQGETTLIAGSGYQQHGSGRWG
                     DYSMLAVDPVDDCTFWYTQEYYATIGTSTWQTRIGSFRFPGCGGPPPPPPPPPPPPTD
                     TPTPTNTPAPTETPIPTDIPTNTPIPPPPPPPPPGTDVVYVSSTSGGTVGGVTFADED
                     ILAYDTTSGTWSMFFDGSDVGLGGSGVDVDAFGILADGTILISLSNAASVTGLGSIQD
                     EDIVRFVPGSLGADTSGNFEWYFDGSDVGLATNSGEDIDAIDFAPDGRLLISTRGSFS
                     VTGASGTDEDLAAFLQTSSGENTAGSWSLYLDGSDVGLNSSSSEDINGVWVNTASGEI
                     YLSTLGSFTVSGATGTGADIFICAPGTLGLTTSCTTFSMYWDASEFGWGSEVTDGIQV
                     AKP"
BASE COUNT          537 a          894 c         1075 g          539 t
ORIGIN      
        1 gccgtcatcg gtctgatgaa gagcctggcc aatgagctcg gcccgaaggg gatccgggtc
       61 aattccgtca acccggcctg gacctggacc gaccggatcg aaaagctcat gaccgatcga
      121 gcccaggcaa acaagaccac cgtcgaagaa gaggcggcca aggcggcggc cgggttgccg
      181 ctcggtcgga tgggcacctt ggaagagtac ggcaagacga tcgcctggct ggcctcgccg
      241 gcggccggct tcgtccacgg ccacgcgttg atgtttgatg gtggtggagt ctccgccccg
      301 atctaaccgc caatgcggat cgcgatcttc ggcaccggcg gcgtaggggg ctactttggc
      361 gggctgctag cgcacagtgg ccacgaggtc acgttcatcg cccgggggga gcaccttcga
      421 gcgatccggg cggccgggct gaaggtgaag agcgtcaacg gggatttcag cgtacaccct
      481 gcccaggcga ccgatgctcc ggccgatatc gggccgatgg cctatgtggt ggtcgcggtg
      541 aaacaatacg acttgcggga ggccgcatca cagatccggc cgctgatcgg gccggaaaca
      601 accgtcgtgc ccctgctgaa cggcgtggac gcccacgagc tgttgattga ggccgtgggg
      661 actggcccgg tggttagtgg gttgtgcagc ttggtctcac tggttgaggc gccaggggta
      721 atccggcagg agagcaaact gcggcgggtg gtggtaggcg agcttgaccg gcggaagtcc
      781 gaccgggtcg agcgactggt gggagcttgg gcggcggccg gggccgaggc gatccatgcc
      841 gaggatatcc tggtggcact gtggaccaag ttcgtgttca ttgcttcctt tgggggcgtg
      901 accgcgctcg ctcgctgcac tgccggtgag ttgctgggaa gctgcgagac ccgggagctg
      961 ttcatccagg ccatgcggga agtcgaggcg gtggcccgcg cccgccacat cgagctggcg
     1021 ccagatgccg ttgagagggg gctatcgatc gcggtcgact tcgaaccgac cgcgacctcc
     1081 tccatgcagc gggacgtggc ggccggcaag ccctttgagc tggaggcctt cagcgggacc
     1141 gtggtccagt tgggcgtgga ggtcggcgtt cccacgccgg tgcaccgcat gatctatggc
     1201 ctgctgaagc cggcgttgct gaaggcgatg ggctagcgag gtactgcagg aggcgagagc
     1261 gccagtgaat tacgtgaaat caaaacctcc cgagggcagg ctggccctcg ggaggtctgc
     1321 tctcggcagc ggatttcagg gtttcgctac ctgaatcccg tcggtgacct cgctgcccca
     1381 gccaaattcg gaggcatccc agtacatgct gaaggtggtg cagctggtcg tgaggccgag
     1441 tgtgccgggg gcgcagatga agatgtccgc gccggtgccg gtcgccccac tcaccgtgaa
     1501 ggagcccagc gtgctcaggt agatctcacc cgaagcagta ttcacccata cgccattgat
     1561 atcttcggat gacgagctgt tcaacccgac atcggacccg tcaagataga gcgaccaact
     1621 tccggcggta ttctcgccag agctcgtctg caggaaggcg gccaggtctt catctgttcc
     1681 tgaggcgccg gtaacgctga aggagccgcg ggtgctgatc aggagacggc cgtcaggtgc
     1741 gaagtcaatc gcgtcgatat cctcgccgct gttcgtggca agtcccacgt cggagccgtc
     1801 gaagtaccat tcgaagttgc cggatgtgtc agcccctaac gacccgggga cgaagcgtac
     1861 gatgtcctcg tcttggatac tgcccaggcc ggtcacgcta gcagcattac tgaggctgat
     1921 caggatcgtg ccatcagcca ggatgccaaa ggcgtccacg tccacgccgc tgccaccgag
     1981 gccgacatct gaaccatcga agaacatcga ccaggtcccg ctcgtcgtgt cgtaggccaa
     2041 gatgtcctca tcggcgaacg tgaccccacc cacggtcccg cctgaggtgg agctgacata
     2101 gaccacgtca gtgccgggcg gtggcggcgg tgggggtggt gggatggggg tattggtcgg
     2161 tatgtccgtc ggaattggcg tctcagtagg tgccggggtg ttcgttggcg tcggtgtgtc
     2221 ggtcggcggg ggtgggggcg gaggcggtgg cggagggggt ccaccgcagc ctgggaaccg
     2281 gaaggacccg atccgcgtct gccaggtgga agtgccgatg gtcgcgtagt actcctgggt
     2341 ataccaaaag gtgcagtcgt ccaccggatc gaccgccaac atgctgtagt cgccccagcg
     2401 gccggagccg tgctgctgat agcctgaacc ggcaatcagc gtagtctccc cttgagccag
     2461 ggttcctgtc gggtctccag cgagccttcc ggcgtagcgg atggaaggat gcaccgaggt
     2521 gctggaggcc gaaaagccga gggcgaggtt gccaaggccg tccatggcgg cactacccat
     2581 ccaacggtgt gtggcatcgg gggagtaggt tccctgctgg aagatcgacc agcctccgcc
     2641 ggagtcgcgc acctcatacc agcggatccc tgcctggtca gccccgttga catcgacggt
     2701 gtggttcgcc accagcgatt ggtggctccc aaagttgcgg tactgcaggc ggtacatcag
     2761 ccggtcgggg atggagtcca ggctagtccc tcccggctgc gggatgcagt tacgcgcgta
     2821 ttcgcataag ttcgagtcga aggcggccgt ggcgagggtc cgatcgagca tgaacgtcga
     2881 gctgaacggg tcaccccaat cggcatggaa gttccagatc tgcaactggt ccggtgagta
     2941 gccccagcca tcgtcgtcga ccagcacgaa ggggttgggt gcgccggccg gaggaatcgg
     3001 cccgtccagg tctgccggca gcagcccgcc caggtcctga tcggt
//
LOCUS       DATDPQ010000100         5289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1612416, whole genome shotgun sequence.
ACCESSION   DATDPQ010000100 DATDPQ010000000
VERSION     DATDPQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612416"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VI520_02255"
     CDS             complement(<1..1236)
                     /locus_tag="VI520_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HLF36736.1"
                     /translation="MSPSPRLYLIDGHALAYRAYYALTRGGDPARWMTASGEPTAGTY
                     GFTSVILRLLEQESPEYLAVSFDIGRTFRDDLFADYKGTREKMPDDLRLQIDRIREVV
                     EAFGIPILEADGYEADDVLGTVARKVSKKGVQVVILTGDRDLLQLASENVIIRLAGQK
                     LAEAIDYGPKQVKQKYQLDPAQLIDLKALVGDTSDNIPGVKGIGEKTAVSLLQDYGDL
                     KGVYRNLAEIPERFRSKLEAGREDAQLSRKLGEIVTNVPIEFDLEDCRARQYDRDRIV
                     PLFRELEFRSLLDRITKQAGESAELYVSEAKAGPDTTVHVVDTPVALESLAARLAKAK
                     SIAFDVETTAPDEMRSELVGISLAIKPGEGYYVPVGHLPDLSGARQLDLSTVITALRG
                     PLTDAKIEKVGHNLKFDFTV"
     gene            complement(1233..2375)
                     /locus_tag="VI520_02260"
     CDS             complement(1233..2375)
                     /locus_tag="VI520_02260"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HLF36737.1"
                     /translation="MTRPSMRRLSWVGVAALLGLAACNFPGLQTPQPEEDSLATAAAQ
                     TVAAQLTQIAGTIGAPSPQPSATVAPLATPSSTPIPSPTTSPTPSCTNQVRFIRDVSI
                     PDNTNIPAGNSFEKIWRLQNSGTCAWTSDYDLVFQSGNIMSGPPSSPLPGTVAPGTEV
                     DIRVSLKAPSADGSHRGNWMLRSADGVLFGIGVNFDQPFFVQIVVGATPTPQPAVVYD
                     FIATYCSAAWVSGTGNLSCPGTDTDIQGFIIKLNAPKLENGITENEPALETHPQWIDN
                     GVITGKFPAFQVKAGDRFKAVIGCLFDGLACNVRFQLNYRADGGALQNLQQWDQTYDG
                     NIQSLDVDLSSLAGKSVEFVLAVLANGPSFQDWAFWLAPRIVGPAR"
     gene            complement(2372..3775)
                     /gene="miaB"
                     /locus_tag="VI520_02265"
     CDS             complement(2372..3775)
                     /gene="miaB"
                     /locus_tag="VI520_02265"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLF36738.1"
                     /translation="MAPVRYHIWTAGCQMNVADSQRVASALEGLGYLPTAVAEQADVV
                     VLNTCVVRQSAEDRAFGRLTSLRPLKERRPEVVINLMGCLVGVRGSPQLEASYPFVDV
                     FSPPSDPQPLIDHLTQADGRSLDKRDTRRRYAAMDGDLLLPLHERGRLVSAHVPVVHG
                     CSHACTFCIIPFRRGPERSRSVGQIVAEVRSLAEQGVREVTLLGQIVDRYGMDVPDGP
                     NLSDLLRAVHRVDGIQRIRFLTSHPNWMSEQLLETVAELEKVCEHIEVPVQAGDDEVL
                     RQMRRGYRADDYRRLIDHIRQWLPGASIATDIIVGFPGESDSQFQRTYDLLAELELDV
                     AHLARYSARPRTVAARRLTDDVPEQEKMRRFQLLEDLQTGIAERSHRRLVGRELEVLV
                     EGKRRGRWMGRSRTNKLVFFEAESEFAGQLVGVKIEHTGPWSLRGSLLHEPLSPQPAA
                     LRPLAVLNHPIAEVSIQ"
     gene            3782..4792
                     /locus_tag="VI520_02270"
     CDS             3782..4792
                     /locus_tag="VI520_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLF36739.1"
                     /translation="MREIQDLVVQLAESVGHGPRRGGLSKPLHGAAWPNPLRASALWV
                     ALISVASCSPLEPPAPATLNPAIAPSASAVSSSSPADPATAPIAAATDPTEVCDQAEG
                     EVEAGTYPAVAVPGELPYLIYLPPCYHQLSRTFPVVYLLHGYPYNERHWLDLGAEQVA
                     NQGLRAGNWPPFMMVMPLQPDPLFRNSDGGPGSYETELLDGIVSYVDKTYRSDPSRRG
                     LAGVSRGGVWALEIAFRNPSDFQAVAAISPALAVNSARPPYDPFEIVSSGENLPREIL
                     LLAGDQDWAASSTERLSLALEVEGFRHSLVIVPGDHSDPTWAEAMPQILAFFAWALAG
                     GP"
     gene            4868..>5289
                     /locus_tag="VI520_02275"
     CDS             4868..>5289
                     /locus_tag="VI520_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HLF36740.1"
                     /translation="MPEDDRLASDVIMAYRRRRERMVPFLLGALAVVLLVVGLVLIVI
                     WFTGDNPPQLPALLQANTATPPPTNTPSPPTQTPPPSPTSAPSETPTPSGPITYVVEE
                     GDTLFSIAEQFGVSIDALIFANTILDPNNIGVGTQLIIP"
BASE COUNT          947 a         1664 c         1692 g          986 t
ORIGIN      
        1 gaccgtaaag tcgaacttga ggttgtgacc gactttctcg atcttggcat cggtgagtgg
       61 accgcgcagc gcggttatca cggtcgacag atcgagctgc cgggctccgg agaggtccgg
      121 aagatggcca accggcacgt agtatccttc gccgggcttg atggccagcg aaatccccac
      181 cagttcggat cgcatctcgt cgggggcagt ggtctcgacg tcaaacgcga tcgacttagc
      241 cttcgccagg cgggccgcca atgactccag ggccaccggg gtgtccacga cgtggaccgt
      301 cgtatccgga ccggccttgg cctcactcac gtacagctcg gccgactcgc cggcttgctt
      361 ggtgatccgg tcgagcagcg aacggaactc aagctcgcga aatagcggga caattcggtc
      421 ccgatcgtat tggcgtgccc ggcagtcctc gagatcgaac tcgattggga cattggtcac
      481 gatctcaccc agcttgcggc tgagctgggc atcttcccga ccggcttcca gtttgctgcg
      541 gaagcgctcc gggatctcgg caaggttccg atagacccct ttcaagtctc cgtaatcttg
      601 aagaagcgag accgcggtct tctccccgat gcccttcacc ccggggatat tgtcactcgt
      661 gtcccccact aaggctttga gatcgatcag ctgggccggg tcgagctggt atttctgctt
      721 gacttgtttt gggccgtagt cgatcgcttc ggccagcttc tggcccgcga gccggatgat
      781 cacgttctcg ctggccaact gaagcaggtc gcggtcaccg gtcaggatga cgacctgaac
      841 gcccttcttg ctcacttttc gggctacggt gccgagcacg tcgtcggctt cgtatccgtc
      901 ggcctccaga atcgggatgc cgaacgcctc tacgacctcc cggatccggt cgatctgcag
      961 tctcaggtca tccggcatct tttcccgggt gcccttgtag tcggcaaaca ggtcgtcgcg
     1021 gaaggttcgg ccgatgtcga acgagacggc caggtactca ggggattcct gttctagcag
     1081 gcgcaggatt accgaggtga agccgtaagt gccggcggtc ggctccccgg aggcggtcat
     1141 ccagcgggcc gggtccccgc cccgggttaa ggcgtagtaa gcccgatagg ccagggcatg
     1201 cccatcgatg aggtacagcc tgggggaggg gctcaccgag ctgggccgac gatccgaggg
     1261 gccagccaga aggcccagtc ctggaaggag gggccgttgg ccagtaccgc gagtacgaac
     1321 tcgactgact taccggcgag cgaggagagg tcgacatcca gggactgaat gttgccgtcg
     1381 taggtctggt cccactgttg aaggttttgg agcgcgccgc cgtcggcccg gtagttcagc
     1441 tggaagcgca cgttgcaggc caatccgtca aacaggcagc cgattaccgc tttgaatcga
     1501 tcgccagcct tcacttggaa agccgggaac ttgccggtga tcacgccatt gtcgatccac
     1561 tgcgggtggg tctcaagcgc cggttcattc tccgtgatcc cgttctctag cttcggcgcg
     1621 ttcagcttga tgatgaagcc ctgaatgtcg gtgtcggtgc cggggcagct caagttgccg
     1681 gttccgctca cccatgctgc ggagcaataa gtggcgatga agtcataaac cactgcaggc
     1741 tgaggagtgg gagtcgcgcc gacaacgatc tggacgaaga atggctgatc gaagttgacc
     1801 ccgatcccga agagcactcc gtctgccgag cgcagcatcc agttgccacg gtgggagcca
     1861 tccgcgctcg gtgccttcag cgagacccgg atgtccactt cggttccggg agcgaccgta
     1921 ccgggcagcg gggaggaagg cgggccggac attatgttcc cgctttggaa taccagatcg
     1981 taatccgaag tccaggcaca agtccccgag ttctgcaatc tccagatctt ctcgaacgaa
     2041 ttccccgcag ggatgttggt gttgtccgga atgctgacgt cgcgaatgaa acggacctgg
     2101 ttggtgcagg agggggttgg actggtcgtg ggcgacggaa tcggagtgga agagggcgta
     2161 gcgagcggag caactgtcgc cgaaggctgg ggggagggcg ctccaatagt tccggcgatc
     2221 tgggtgagct gggcagcgac cgtctgggcg gctgcagtgg cgagactatc ttcttcgggc
     2281 tgaggggtct gcaatcccgg gaagttgcag gcggcgagcc cgagcagtgc ggccacacca
     2341 acccagctga gccggcgcat ggatggtcga gtcattggat agaaacctct gcaatcggat
     2401 ggttcagaac ggcaagcggt cggagagcgg ccggttgcgg ggaaagtggc tcgtggagaa
     2461 gcgaaccgcg caggctccat ggaccagtgt gctcaatctt caccccgacc agctgcccgg
     2521 cgaactcgct ctccgcctcg aagaagacta gcttgttggt gcggctgcgt cccatccagc
     2581 ggccccggcg cttgccttcc accagcacct cgagctcgcg gccaaccagc cggcgatggc
     2641 ttctctccgc aatgccggtc tgcaggtcct cgagcagctg gaatctgcgc atcttctctt
     2701 gctccggcac atcgtcagtt aggcgccggg cggcaacggt ccgcggccga gcggagtacc
     2761 gcgccagatg ggcgacatcg agctcgagtt cggcgagcag atcgtaggtg cgctggaact
     2821 gggaatcgct ctcgcccggg aatccgacga tgatgtcggt tgcgatcgag gcgccgggca
     2881 gccactgcct aatgtggtcg atcaggcggc ggtagtcgtc ggcgcgatat ccgcggcgca
     2941 tctggcggag cacttcatca tcgccggcct ggaccgggac ttcgatatgt tcacagactt
     3001 tctcgagctc ggcgacagtc tctagcaatt gctccgacat ccaattggga tgggaggtca
     3061 ggaagcggat ccgctgaatt ccatcgactc gatgcaccgc ccgcagcaaa tcggacagat
     3121 tggggccgtc cgggacatcc attccatagc gatcgacgat ctgacccagc aaggtgactt
     3181 cccgcactcc ctgttccgcc agggaacgga cctcggccac gatctgccca acactccggc
     3241 tccgttccgg cccgcgccgg aaggggatga tgcagaaagt gcaggcatgc gagcatccgt
     3301 ggacgaccgg cacgtgagcg ctgaccaggc gaccgcgctc gtggagcgga agcagcaagt
     3361 ccccatccat ggccgcatag cgacgccggg tgtcccgctt gtcgagcgag cggccgtcgg
     3421 cctgggtgag atggtcgatc agtggttggg gatcggaggg cggcgagaaa acatcgacaa
     3481 atgggtaaga agcctcgagt tgcgggctgc ccctcacgcc caccaggcag cccatcaggt
     3541 tgatgacgac ttccggccgc cgctccttga gcggccgaag ggaagtcagc cgcccgaagg
     3601 cccgatcctc ggcgctctgg cgcaccacac aggtgttcag gaccacgaca tcggcctgtt
     3661 cggcgacggc ggtcgggagg tagcccagcc cctccagcgc agaggcgacc cgctgtgaat
     3721 cagcaacgtt catctggcag ccggcggtcc agatatggta gcgcacgggc gccattatac
     3781 tgtgcgcgaa atccaggacc tggtggtcca gttggcagaa tcggttgggc acggacctcg
     3841 aagaggcggc ttatcaaagc cacttcacgg cgcggcatgg ccgaatccgc tcagagcttc
     3901 ggccctgtgg gttgccctga tttcagtagc cagctgcagt ccgctcgagc ccccggcccc
     3961 ggccaccctg aaccccgcga tcgcgccatc cgcttcggcg gtctcgtctt ccagcccggc
     4021 cgatccggca accgccccaa tcgcggcggc gaccgatccg actgaagtct gtgatcaggc
     4081 ggaaggagaa gtcgaggccg gcacctatcc agcggtggca gtgcccggcg agctcccgta
     4141 cctgatctac ctcccccctt gctaccatca actcagccgg accttcccgg tggtctactt
     4201 gctgcatggc tacccgtaca acgagcggca ctggctggat ctcggagcgg aacaggtcgc
     4261 caatcaaggg ctccgggcgg ggaattggcc gccgttcatg atggtgatgc cattgcagcc
     4321 cgacccgctc ttccggaact ccgatggtgg gccgggctcg tacgaaaccg aactgctgga
     4381 cggcattgtc tcttatgtcg acaagactta tcgctctgat ccctcccgcc gcggcttggc
     4441 cggggtttcg cggggcgggg tctgggcgct cgagattgcc ttccgcaacc cgtccgactt
     4501 tcaggccgtg gccgcgatca gcccggccct ggcggtcaac tccgcccggc caccctatga
     4561 cccctttgaa atcgtcagct ccggggagaa tctgccccgg gagatcctgt tgctcgcagg
     4621 tgatcaggac tgggctgcca gctcaaccga gcggctcagc ctggccttag aagtggaggg
     4681 cttccggcac tcgctcgtga tcgtgccggg ggaccacagc gacccgacct gggccgaggc
     4741 catgccccag atcctcgcct tcttcgcctg ggcgctggcc ggaggaccct gatcggggtc
     4801 gtaagcgctc cgggaaacgg tttcaggtac aatggccggc ctggaggggg gatcactgcc
     4861 tgcacaaatg cccgaagacg atcggttggc ttcggatgtg atcatggcct accggcgccg
     4921 ccgggagcgg atggtgccat tcctgctcgg ggcattggcg gtggttctgc tggtggtggg
     4981 cctggtcctg atcgtcatct ggtttaccgg cgataacccg ccccagctcc cggctctgct
     5041 tcaggccaat acggctaccc ccccgccgac caacaccccg agtccgccca ctcaaactcc
     5101 acctccgagc cccacctccg cgccctctga aacgcccacc ccgtccggcc cgatcaccta
     5161 tgtggtcgag gaaggcgata cgctgttttc gatcgccgag cagttcgggg tgagcatcga
     5221 tgctctgatt tttgccaaca cgatcctcga tccaaacaac atcggagtcg gaacccagct
     5281 catcattcc
//
LOCUS       DATDPQ010000101         5334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2648403, whole genome shotgun sequence.
ACCESSION   DATDPQ010000101 DATDPQ010000000
VERSION     DATDPQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648403"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VI520_02280"
     CDS             complement(<1..397)
                     /locus_tag="VI520_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLF36741.1"
                     /translation="MIRIGLIGAGRIGQIHGRNLATQLPGVRLAAVADLDRAAADSLA
                     ERYAVPLVTGDPHELLQDAELAAVAICSPTDTHAGLIQQAAAAGKHIFCEKPIDFELG
                     RIDQALAAVAAAGIKLQIGFNRRFDPNFRK"
     gene            complement(397..1866)
                     /locus_tag="VI520_02285"
     CDS             complement(397..1866)
                     /locus_tag="VI520_02285"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HLF36742.1"
                     /translation="MEIVKNYIDGAWRESQAQEWLDVPNPATGEVLGQVPLSPAGEVD
                     HAAQAAAAAFQEWRRTPAVERVQHLFKLKDLLERHFEELARTITLENGKTLEESRGEV
                     RRAIENVEVACGIPILMQGVVSEDIARGIDELMIRQPVGVGAALCPFNFPAMIPFWSM
                     PYALACGNTFIVKPSERVPLTMVRIFELMEQVDFPRGVVNLVHGSRLTAEAVLDHPGI
                     QAISFVGSTPVAKAVYTRAAASGKRAQVQGGAKNPIIVLPDADLESTTRIVADSAFGS
                     AGQRCLAASLAVTVGEARDGFVEAIAEAAAQRVVGDGLVEGVQMGPVISSASRARIER
                     LIGEGEAEGARVMVDGRGVKVPGYEGGFFLRPTVMVDVPAQGATFKTEIFGPVLGLLH
                     VPTIEEAIELVNSGQYGNMACLFTSSGAAARKFRYEAEVGNIGINIGVAAPMAFFPFS
                     GWRESFFGVLHGQGRDAVEFYTQEKVVVERWPKEWSRKF"
     gene            complement(1927..2946)
                     /locus_tag="VI520_02290"
     CDS             complement(1927..2946)
                     /locus_tag="VI520_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLF36743.1"
                     /translation="MTVRVGLIGGGRMGSVLARYLANGVPEAKLVAIADQKLERAEAL
                     AAQLSAEAAYSNPAEIIDRRDIEAVLLVTPSETHPDLVELAANAGKHIFVEKPLALTL
                     EGCDLAIAAAAAAGVKLQVGFMRRFDIAHRTARQKIEEGAIGRPVLFKSISRDRERTS
                     LEFARRESSGGLIMDMGAHDFDTARWLMGSEVVRVHSEGAALAYPELEQVGDIDNAVV
                     NLQFADGAVGNVDLSRNAVYGSDFRTEVLGSKGAVVIGGLGQAELTVLSAGSRAQGYD
                     GGRFEAAYIEELQHFVNCVARDEPPLVTGAEARQATAIAVAATRSLDEGQAVFLSEIG
                     RPGPG"
     gene            complement(2984..3781)
                     /locus_tag="VI520_02295"
     CDS             complement(2984..3781)
                     /locus_tag="VI520_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLF36744.1"
                     /translation="MAGFQVSAEHRKRAETFNREWDLEVSRLYGLEHGPPISQSEVIG
                     AVNAAAGPRDVVLGAAGSLPGDLHKLWRTRDPKGYHLEYGYSCMGYEIAGGLGVKMAA
                     PDREVYVMVGDGSYLMMNSEIVTSIQEHYKLIIVLMDNHGFSSIGGLSQAVGSGGFGT
                     RYRHRHPASGQLDGELLPVDFAANARSLGAQAIQAADLPSLKAALERAKDEKRTTVVV
                     IETDPEQRVPGYESWWDVPVAETSEIASVRSAREVYEAGRRRERPPI"
     gene            complement(3803..4846)
                     /locus_tag="VI520_02300"
     CDS             complement(3803..4846)
                     /locus_tag="VI520_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLF36745.1"
                     /translation="MANVRLTMAQAIIRFLKSQYVERDGLERAFFAGCWGIFGHGNVA
                     GIGEALQQNPDFRYYLTRNEQAMVHLAAAFAKMNDRLQTFACTSSIGPGATNMVTAAA
                     GATINRLPVLLLPGDLFARRNAAPVLQQLEQTYTQDISVNDCFKPISRYWDRISRPEQ
                     IIASLPEAMRILTSPAETGAVTLSLPQDVQAEAYDYPAELFEKRVWQVPRPRPDIELL
                     QQAAHWIREAEAPIIIAGGGVIYSQASEALAALAEHSGIPVAETQAGKGALRYNHPQS
                     LGAMGVTGTPGANVLAREADLVIGIGTRYSDFTTASKTAFQHPEVRFININVAEFDAH
                     KHAALPLVGDARV"
     gene            complement(4918..>5334)
                     /locus_tag="VI520_02305"
     CDS             complement(4918..>5334)
                     /locus_tag="VI520_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLF36746.1"
                     /translation="EQEIKATALEQQDQLVIQHSQLSEPEVVGQLDPAARMLLEQGPG
                     AVVIKRGAHGAAVYLSNGEVVEAPGFRVQVYKVLGAGDAFASGLIYGVLKGWDWYRSI
                     RMGNACGAIIVTRPGCANFMPYEQEALDFVEQHGGF"
BASE COUNT          964 a         1821 c         1560 g          989 t
ORIGIN      
        1 ccttgcggaa attggggtcg aaccttcggt tgaaaccaat ttggagctta attccggcgg
       61 ctgctaccgc ggccagggcc tggtcgatcc gacccaattc gaagtcaatc ggcttctcac
      121 aaaaaatgtg cttgccggcc gcagccgcct gctggatcag cccggcgtgg gtgtcggtgg
      181 gcgaacaaat ggcgaccgcc gccagctcgg cgtcctggag cagttcgtgc gggtcaccgg
      241 tcaccagcgg aaccgcgtac cgctcggcca gcgaatcggc tgcggcccgg tcgaggtcgg
      301 cgaccgcggc cagccggact ccagggagtt gggtggccaa attgcggccg tggatctggc
      361 caatccggcc ggctccgatc aggccgattc tgatcattag aacttgcggg accactcctt
      421 cggccagcgt tccacgacca ccttctcctg ggtgtagaac tccaccgcgt ctcgtccttg
      481 tccatgcagg actccgaaaa aactctcccg ccagccgctg aagggaaaga aggccatcgg
      541 ggcggccact ccgatgttga tcccgatgtt gccgacctcg gcctcgtaac ggaacttgcg
      601 ggccgcggca ccgctgctgg tgaagagaca ggccatgttt ccgtactgcc cgctgttcac
      661 caactcgatc gcttcctcaa ttgtcggtac gtggagcagc ccgagcactg gcccgaaaat
      721 ctccgtcttg aacgtggcgc cctgagccgg aacatcgacc atcaccgtcg gccttaggaa
      781 aaaccctccc tcgtagcctg ggaccttaac tccccggcca tctaccatca cccgggcgcc
      841 ttcggcctcg ccctcaccaa tcaatcgctc gatgcgggcc cggctggcgg agctaatcac
      901 aggccccatc tgcaccccct ccaccaggcc atcgccgacg acccgctgcg ccgccgcttc
      961 ggcgatcgcc tcgacaaaac cgtcgcgggc ctcgcccacc gtcaccgcca gggaggccgc
     1021 caagcagcgc tgaccggcgc tgccaaaggc gctatcggca acgatccggg tagtggattc
     1081 caggtcggca tccggcaaga cgatgatcgg attcttggca ccgccctgga cttgggcccg
     1141 cttgcccgaa gcggccgccc gcgtgtacac cgccttggcc accggggtcg atccgacaaa
     1201 actgattgcc tgaatgccgg ggtggtcaag gacggcttcg gcggtcaggc gcgatccgtg
     1261 gaccaggttc accacccccc gcgggaaatc gacctgctcc atgagctcga atatccggac
     1321 catcgtaagc ggaactcgct cggagggttt gacgatgaag gtattcccgc aggcaagggc
     1381 atagggcata gaccagaacg gaatcatggc ggggaagttg aacggacaca gcgccgcgcc
     1441 tactccgacc ggctgacgga tcatgagctc atcgatcccc cgggcaatgt cctccgatac
     1501 aacaccctgc atcaggatag ggatgccgca ggcgacctcc acgttttcga tcgcccgccg
     1561 cacttccccc cgggactctt caagagtctt gccattctca agagtgatcg tgcgggccaa
     1621 ctcctcgaaa tggcgctcca gcaggtcttt cagtttgaag agatgctgca cccgctcgac
     1681 cgccggcgtg cgacgccatt cctggaacgc cgccgcggcg gcctgcgccg cgtgatcgac
     1741 ctcccccgct ggtgaaagcg gcacctgacc aagcacttcg ccggtggcgg gatttggcac
     1801 gtctaaccat tcctgagcct gtgactcgcg ccaggcccca tcgatgtagt tctttacgat
     1861 ttccatctga ccaactcccc aaggccaggc aagcggcctt gaaccaaccg ggcgattgtg
     1921 ggcggctcag ccaggcccgg gccggccgat ctcgctcagg aacaccgcct ggccttcatc
     1981 gagggatcgg gttgccgcga ccgcgatggc agtcgcctga cgggcctcgg ctccagtcac
     2041 caatggcggc tcgtcccgcg ccacgcagtt cacgaagtgt tggagctctt cgatataggc
     2101 cgcctcgaac cgccccccgt cgtacccctg cgcccgactt ccggccgaca ggaccgtcaa
     2161 ctccgcctga cccaagcctc cgatgaccac tgcccccttc gatcccagca cttcggtccg
     2221 gaaatcggat ccatagactg cattgcggct gaggtcaaca ttccccactg caccgtccgc
     2281 aaattgaaga ttgactaccg cgttgtcgat gtccccgacc tgctccagtt ctggataggc
     2341 cagcgcggct ccctcgctat gaacccgcac gacctcgctg cccatcagcc agcgggccgt
     2401 atcaaaatcg tgtgcgccca tgtccatgat caatccaccg ctggactcgc gcctggcaaa
     2461 ctccaatgag gtgcgctccc gatctcggct aatcgactta aacagcaccg gccgaccaat
     2521 tgccccttcc tcgatcttct gtcgagcagt gcggtgggcg atgtcgaagc ggcgcatgaa
     2581 accaacttgc agttttaccc cggcggccgc ggcagctgcg atggccaagt cgcagccttc
     2641 gagcgtgagt gccaggggct tttcaacaaa gatgtgtttc cctgcattgg cagccaattc
     2701 aaccaggtcc ggatgggttt cgctcggggt aaccagcagc acggcctcga tgtcccgccg
     2761 atcgatgatc tctgcgggat tcgagtatgc ggcttcggcg ctgagctgcg ccgccagcgc
     2821 ttcggcccgc tccagcttct gatcggcgat cgctaccagt tttgcctctg ggaccccgtt
     2881 cgccagatat cgagccaaga cgctccccat tcgcccaccg ccgatcagtc caacccggac
     2941 agtcatctgc gggcgctccg gccggaggcg atctccacat ttctcaaatc ggggggcgct
     3001 ccctccggcg gcccgcctca tacacctccc gggctgaccg gacactggcg atctcggaag
     3061 tctcggcgac cggcacgtcc caccacgatt cgtaacccgg gacgcgctgt tccggatcgg
     3121 tctcgatcac caccaccgtg gtccgcttct cgtccttcgc tcgttcaaga gcggctttga
     3181 gtgaggggag gtcagccgct tggatcgcct gggcgccgag gctgcgggca ttggcggcaa
     3241 agtcgaccgg caggagctct ccgtccagct ggccgctcgc cgggtggcgg tgacggtaac
     3301 gtgtcccgaa ccctccactt cccaccgctt gcgagagccc tccgatgctt gagaaaccgt
     3361 ggttgtccat gagcacgatg atcaacttgt agtgttcctg gatggaagtc acgatctccg
     3421 aattcatcat cagataactg ccgtcgccga ccatgacgta cacctctcgg tcaggggcgg
     3481 ccatcttcac ccccaggcct ccggcgatct catagcccat gcaagaatac ccgtactcca
     3541 ggtggtaccc ctttgggtcg cgggttcgcc acagcttgtg caagtccccc ggcaggcttc
     3601 ccgccgcgcc gaggacgacg tcgcgtgggc cggccgcagc attcaccgct ccaatcacct
     3661 cgctttggct gatcggagga ccgtgctcga ggccgtagag acggctcact tccaaatccc
     3721 attcccgatt gaaggtctcc gcccgtttcc ggtgttcggc cgacacttgg aagccggcca
     3781 atagggtcga tagctcctca agtcagaccc gggcgtcccc gaccaatggc aaagcagcgt
     3841 gcttgtgagc gtcaaactcg gccacgttga tattgatgaa tcgcacctcg gggtgctgaa
     3901 aggcagtctt ggaggcagtg gtgaagtcgc tgtagcgggt accgataccg atcaccaggt
     3961 ctgcctcgcg ggccaggaca ttcgcccccg gcgtgccggt gacgcccatc gcgccaagtg
     4021 actgcggatg gttgtagcga agcgcgcctt ttccggcctg ggtctcggcc accggaatcc
     4081 ccgaatgctc ggcgagcgcg gccaacgctt cggaggcctg gctgtagatc acgccgccgc
     4141 cggcaataat gatcggggcc tcggcttccc ggatccagtg agcggcttgc tgcagaagtt
     4201 caatgtccgg ccgcggccgc gggacctgcc agacccgttt ctcgaataac tcggccggat
     4261 agtcgtaggc ttcggcctgt acatcctgag gcagcgagag agtgaccgcg ccggtctcgg
     4321 ccggcgaggt caggatccgc atggcctccg gcaaggaggc gatgatctgt tccgggcgac
     4381 tgatccgatc ccagtaccgc gaaatcggct tgaagcagtc gttcaccgat atgtcctggg
     4441 tgtaggtctg ctccaactgc tgcagcacgg gggccgcgtt acgccgggcg aagaggtcgc
     4501 ccggcaggag caagacggga agccggttga tggtcgcgcc ggccgcagcg gtgaccatgt
     4561 tggtagcgcc tggcccgatc gaagaagtgc aggcaaatgt ctgcagccgg tcgttcattt
     4621 tcgcgaaggc ggccgccaag tggaccatgg cttgttcgtt gcgagtcagg tagtagcgga
     4681 aatccgggtt ttgttgcagc gcttcgccga tccccgcaac gttgccgtgg ccgaagatgc
     4741 cccagcagcc tgcaaagaac gcccgctcaa gtccgtcccg ctcaacatac tggctcttca
     4801 agaaccggat gatggcctgg gccatcgtca atcgcacatt agccatcccg tctcacctct
     4861 cgcagagcgc cgcattcggc tgcggaattt gtcagcggac gcgccctgca cccggagcta
     4921 gaagccaccg tgctgctcga caaaatcgag tgcctcctgc tcgtaaggca tgaagttggc
     4981 acatccgggg cgggtcacga tgatcgcccc gcatgcattg cccattcgga tcgatcgata
     5041 ccaatcccaa cccttcagca ctccgtagat caggccgcta gcgaaggcat cccccgcgcc
     5101 cagaacctta tacacctgga cccggaagcc cggagcttcc acaacctctc catttgacag
     5161 atataccgcc gccccgtgtg cgccccgctt gatcaccacg gcccccggcc cctgctcgag
     5221 caacatgcgg gctgcgggat ccagctggcc gaccacctcc ggctcgctta attgggaatg
     5281 ctggatcacc agctggtctt gctgctcgag ggctgtcgct ttgatctcct gctc
//
LOCUS       DATDPQ010000102         7592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_38401, whole genome shotgun sequence.
ACCESSION   DATDPQ010000102 DATDPQ010000000
VERSION     DATDPQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38401"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..652
                     /gene="rplC"
                     /locus_tag="VI520_02310"
     CDS             23..652
                     /gene="rplC"
                     /locus_tag="VI520_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HLF36747.1"
                     /translation="MKGLIGRKLGMTQIFDDTGAAIPVTLIEAGPCVVTQIRRPASEG
                     YAAVQLGFGVAKPKRLSGGQLGHLKRGASPPLRYLREFRVKNVEEFHEGAELTVRVFE
                     VGDRVDIVGISKGRGFAGVIRRHHFSRQPKTHGASDRERAPGSSSSTTTPGRVFKGKR
                     GPGHMGSVRVSSQNLRVDLVDPERNLLGVRGSVPGATGDVVVISERRKQ"
     gene            703..1341
                     /gene="rplD"
                     /locus_tag="VI520_02315"
     CDS             703..1341
                     /gene="rplD"
                     /locus_tag="VI520_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HLF36748.1"
                     /translation="MKIDVLNMRGEKVDTAELPEAVFKVQVKPDLMHQAFLRQQANAR
                     LGTHKTKTRAEVAGGGIKPWRQKGTGRARHGSIRSPIWVGGGKAHTPRPRDYEQRMPR
                     KMRRAALRSALSLKAGNQQITVLDELKLSGPKTKEIAELLRNLSGGQSALILLPEKLE
                     SVEKSVRNLVEFKTVRAQYVNVRDLLQFERLILPLQAVGMLQAILDPGVKSK"
     gene            1338..1640
                     /gene="rplW"
                     /locus_tag="VI520_02320"
     CDS             1338..1640
                     /gene="rplW"
                     /locus_tag="VI520_02320"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLF36749.1"
                     /translation="MTGLYEVLHRPIVTEKSNFLSSKLGQYVFEVAVEANKAEIKEAV
                     ETLFDVSVVKVNVLNSPAKRTRRLKNRRLLVRRSGYKKAVVTLASGQRIDLFEGVA"
     gene            1640..2476
                     /gene="rplB"
                     /locus_tag="VI520_02325"
     CDS             1640..2476
                     /gene="rplB"
                     /locus_tag="VI520_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLF36750.1"
                     /translation="MAVRKYKPTSPGRRGMTGDLFEEVTKSTPERALIQRRKARGGRN
                     RQGRLTVRHRGGGHQRFLRKVDFQREKRGVPARVAAIEYDPNRSARLALLHYSDGEKR
                     YILAPFGLGVGDTIMAGPTADIRPGNSLPLASIPLGTLIHNIELQEGRGGQMARSAGA
                     AAQLLGKEGEFAAIRLPSGEVRRIRQDCYATIGEVGNPDHANLKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRSPIGMPSPKSPWGKPTLGKKTRRNKRSDREIIRRRAKK
                     GR"
     gene            2491..2784
                     /gene="rpsS"
                     /locus_tag="VI520_02330"
     CDS             2491..2784
                     /gene="rpsS"
                     /locus_tag="VI520_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HLF36751.1"
                     /translation="MSRSLKKGPFIQPKLLKRVEEMNQRGEKKVIRTWSRASTIFPQM
                     VGHTIAVHDGRRHIPIYITENMVGHKLGEFAPTRTFRAHSGREKTTTTEEVVA"
     gene            2781..3140
                     /gene="rplV"
                     /locus_tag="VI520_02335"
     CDS             2781..3140
                     /gene="rplV"
                     /locus_tag="VI520_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLF36752.1"
                     /translation="MTDGLDVRATHRYLRMSPTKVRLVLDQIRGQSAVEAIQVLKFTN
                     KAAADPLSKLLKSAIANAEQNFGLNRDNLYVHQAFANEGPIRRWRRFGARVHFKPIRK
                     RQAHVTLVLRDRGEPTT"
     gene            3190..3864
                     /gene="rpsC"
                     /locus_tag="VI520_02340"
     CDS             3190..3864
                     /gene="rpsC"
                     /locus_tag="VI520_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLF36753.1"
                     /translation="MGRKVHPIGFRLGVNRSWDSRWIAKRPAYVAQLNEDRLIREVVH
                     KTTPRAGISQVEIERFANAVHVTVHTSKPGIVIGRKGANVKELRTKLQEATGTSIKLD
                     IAEIKEPDLDAQLVAENVANQLERRISHRRAMQRAVDSAMRAGGKGIKVMCAGRLAGS
                     EMARRDWVREGRVPAQTLRADIDFARAEAMTTYGRIGVKVWIYRGEILPEAEGQPETM
                     DVYVSE"
     gene            3875..4300
                     /gene="rplP"
                     /locus_tag="VI520_02345"
     CDS             3875..4300
                     /gene="rplP"
                     /locus_tag="VI520_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HLF36754.1"
                     /translation="MLMPKRLKWRKQMRGRMKGTASRGTRVEFGEYGLQSMEPGWISA
                     RQIEAARRALVRYMKRRGKVWIRIFPDKPVTQRAAETRMGKGKGPVDHWVAVVQPGRI
                     LFEVSGLDRAAAQQAMRLASHKLSVKTKFAARVESVSAE"
     gene            4332..4529
                     /gene="rpmC"
                     /locus_tag="VI520_02350"
     CDS             4332..4529
                     /gene="rpmC"
                     /locus_tag="VI520_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HLF36755.1"
                     /translation="MKAKELRELTVEQVAEKLEAAREAYFKLRFQFSTGQLPNTAQLQ
                     VARREVARITTVLQEMERASE"
     gene            4526..4792
                     /gene="rpsQ"
                     /locus_tag="VI520_02355"
     CDS             4526..4792
                     /gene="rpsQ"
                     /locus_tag="VI520_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLF36756.1"
                     /translation="MRNLRRRLTGEVVRAAMQKTITVRVDRTFRHPLYQKVLRRSTQY
                     LVHDELGCLPGDQVKIVETRPISRRKRWMVEEILRRGSPVEMVE"
     gene            4789..5160
                     /gene="rplN"
                     /locus_tag="VI520_02360"
     CDS             4789..5160
                     /gene="rplN"
                     /locus_tag="VI520_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLF36757.1"
                     /translation="MIQQESRINIADNTGGRTMEVIRVLGGSKRRYGRVGDLVVGTIK
                     SAVPNSAVRKSEIVKAVIVRTAKEYRRADGSYIRFDDNAAVVLDADKVNPKGTRIFGP
                     VARELRERGFMKIISLAPEVI"
     gene            5172..5498
                     /gene="rplX"
                     /locus_tag="VI520_02365"
     CDS             5172..5498
                     /gene="rplX"
                     /locus_tag="VI520_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLF36758.1"
                     /translation="MKVKLKRGDTVEVISGRGKGTRGEVIRVVPDGRGIVVRGVNLRK
                     KHQRQIQAAGRSMSPGIIQFEAPINVSNVMLVCPKCGKLTRLAIHRTDGQAQRFCKKC
                     ENQVDG"
     gene            5503..6060
                     /gene="rplE"
                     /locus_tag="VI520_02370"
     CDS             5503..6060
                     /gene="rplE"
                     /locus_tag="VI520_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLF36759.1"
                     /translation="MDHYLQKRYREVIVPRLMQDFGLANQMEVPRLRKIVVNVGAGEA
                     MDNAKAMDAAVADLSAITGQHPIVTRAKKSIANFKLREGHSIGAKVTLRGERMWSFFD
                     RLVNVALPRTRDFRGLSPNSFDGRGNYTLGMREQLVFPEIEYDKIDKLRGFEVTVVTS
                     APNDEQGRRLLTLLGMPFQRTESNG"
     gene            6053..6235
                     /locus_tag="VI520_02375"
     CDS             6053..6235
                     /locus_tag="VI520_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HLF36760.1"
                     /translation="MAKKCMPYRELRRKYKVRVRNRCHICGRPRGYYRRFDLCRICFR
                     EQALRGNIPGVVKASW"
     gene            6282..6689
                     /gene="rpsH"
                     /locus_tag="VI520_02380"
     CDS             6282..6689
                     /gene="rpsH"
                     /locus_tag="VI520_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLF36761.1"
                     /translation="MTISDPISDMLTRVRNGALAGQQLVSMPSSKLKLEISRILKEEG
                     YIMSFESAQENDHPILRIRLKYVGERRARRPVITGLRRISRPGSRTYVSRYKIPWVRS
                     GMGIAILTTPKGVMTGIRARQLGVGGELLCEVW"
     gene            6707..7255
                     /gene="rplF"
                     /locus_tag="VI520_02385"
     CDS             6707..7255
                     /gene="rplF"
                     /locus_tag="VI520_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLF36762.1"
                     /translation="MSRIGRSPVAIPEGVEVRIEGQRVRVKGPKGELEQVFRDEMGIT
                     EDQGVLHVTRNSDQPSVRALHGMTRALLNNMILGTTKGFTKTLEIQGVGYRAELKGST
                     LMLYVGFSHSVEVTPPPGIKFEVEAKGDVVRILGYDKQLVGQVAADIRNVRPPEPYKG
                     KGIRYHGEHVRRKAGKAGKVTQ"
     gene            7259..>7592
                     /gene="rplR"
                     /locus_tag="VI520_02390"
     CDS             7259..>7592
                     /gene="rplR"
                     /locus_tag="VI520_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLF36763.1"
                     /translation="MASATRAVARQRRQRRVRAKVSGTPRVPRLNVFRSLDQIYAQLI
                     DDSRGHTLVSASTVDPELRPKAAGLRKLEQAKLVGELLAQRAKAIGMDRAVFDRGGNR
                     YHGRVKAVA"
BASE COUNT         1635 a         2131 c         2567 g         1259 t
ORIGIN      
        1 acatcgagat caagctctag cgatgaaggg acttatcggc aggaaactgg gaatgaccca
       61 gatttttgat gacaccgggg cggccattcc ggtgacgctg atcgaggccg gcccgtgtgt
      121 cgtgactcag atccgccggc cggccagcga agggtacgcg gcagtgcagc tcggcttcgg
      181 ggtggccaaa ccgaaacggc tgagcggcgg ccagctgggc cacttgaaac gcggggcttc
      241 cccgccgctg cgctacctgc gcgaattccg ggtcaagaac gtggaggagt tccacgaagg
      301 ggccgaactc acggtaagag tgttcgaggt tggggaccga gtggacatcg tcgggatcag
      361 taaagggcgt gggtttgccg gggtcatccg gcgccaccat ttcagccggc aaccaaaaac
      421 ccacggcgcg tccgatcgtg aacgggcgcc cgggtcgagc agctcgacta cgactccggg
      481 acgggttttc aaaggcaagc gcgggccggg ccacatgggc tcggtgcgag tcagctcgca
      541 gaacttgcgg gtggatctgg ttgatccgga gcgtaatctg ttgggggtgc gcggcagtgt
      601 gcccggtgcg acgggtgatg tggtggtgat cagcgagcgt cgaaagcagt agccgaccct
      661 cggtttgcgg attttccgga acgccggagc ggaagaaacc aagtgaagat agacgtgctc
      721 aacatgcggg gcgagaaagt ggacaccgcc gaactgccgg aggcggtctt caaggttcag
      781 gtcaagccgg atctgatgca tcaggcgttc ctgaggcagc aggccaatgc ccgcttggga
      841 acccataaga ccaagacccg ggcggaagtt gcgggaggtg gaatcaagcc ctggaggcag
      901 aaaggtaccg gccgggcacg ccatggctcg atccgatcac cgatctgggt gggcggaggc
      961 aaggcccaca cgcctcggcc gcgtgattat gagcagcgga tgccgcgcaa gatgcggcgg
     1021 gccgcgctgc ggtcggccct ctcgctgaag gccggtaacc agcagatcac ggtcttggac
     1081 gagctgaagc tgagcggacc gaagacgaag gaaatcgcgg agctgctgcg aaatctgagc
     1141 ggcggtcaga gtgccctgat cctgctcccg gagaagctcg agagcgtcga gaagtcggtg
     1201 cgcaacctgg tcgaattcaa gaccgtgcgg gcccagtatg tcaatgtgcg tgacttgctc
     1261 cagttcgagc ggttgatcct gccgcttcag gcggtcggga tgctgcaggc catcctggat
     1321 ccgggagtca agtccaaatg actggattgt atgaggtgct gcaccggccg atcgtcaccg
     1381 agaagtccaa cttcctgagt tcaaagcttg ggcaatacgt ttttgaagtg gcagtcgaag
     1441 cgaacaaggc ggagatcaag gaagctgtcg aaacgctctt tgacgtgagc gtggtcaagg
     1501 tcaatgtgct caacagcccg gccaagcgca ctcgccgcct caaaaatcgg aggctgctgg
     1561 tccggcggtc cggttacaag aaggccgtcg tgaccctggc ctccgggcag cggatcgact
     1621 tgtttgaggg agtggcctga tggcagttcg caaatataag ccgacctcgc ctggccgtcg
     1681 gggtatgact ggcgacctct tcgaggaggt caccaagagc accccggaac gggcgttgat
     1741 ccagcgccgc aaagcccgcg gcgggcgcaa ccggcaaggg cggctgaccg ttcgccatcg
     1801 gggtggcgga caccagcgct tcttgcgcaa ggtggacttc cagcgagaga agcggggagt
     1861 accggctcgg gtcgcagcga ttgagtacga ccccaatcga tcggcccgcc tggcgctgct
     1921 gcattattcc gatggtgaga agcgctacat cttggccccc ttcggtctgg gcgtcggcga
     1981 cacaatcatg gcagggccga ccgccgacat tcggcctgga aacagcctgc ctttggcgag
     2041 catcccgctc ggtaccctga tccacaacat cgaactgcag gagggtcgag gagggcagat
     2101 ggcacgctcg gccggcgcgg ccgctcagct gctcggcaag gaaggcgagt ttgcggcgat
     2161 tcggttgccg tccggcgaag ttcgccggat caggcaggat tgctacgcca caattggtga
     2221 agtcgggaac ccggaccacg ccaacctcaa actgggcaag gcaggccgca agcggcattt
     2281 ggggatccgg ccgaccgtcc gcggaaccgc gatgagcccc cgggaccacc cccatggggg
     2341 tggcgagggc cgctccccga ttggcatgcc gtcaccgaag agtccctggg ggaaaccgac
     2401 cctgggcaag aaaactcgtc gaaacaagcg cagcgaccgg gagatcattc gccggagggc
     2461 caagaagggc cgctaggccg aggtatctaa atgtccagat cattgaagaa gggacccttc
     2521 atccagccga aattgctcaa gcgggtggaa gaaatgaacc agcggggcga gaagaaggtc
     2581 atccggacct ggagccgggc cagcacgatc tttccccaga tggtcggcca cacgatcgcg
     2641 gtgcatgacg ggcggaggca cattccgatc tacatcaccg aaaacatggt cggccacaaa
     2701 ctgggggagt tcgccccgac ccgcaccttc cgggcccact cgggcaggga aaagacaacc
     2761 acgaccgaag aggttgttgc atgacggacg ggctcgacgt acgggcgact caccgatact
     2821 tgcggatgtc gcctaccaaa gttcggctgg tgctcgatca gatccgcggc cagagtgcgg
     2881 tcgaggcgat ccaggtgctc aagttcacaa acaaggcggc cgccgacccg ctctcgaagc
     2941 tgttgaaatc ggctatcgca aacgcggaac agaatttcgg cttgaaccgc gacaatctgt
     3001 acgtgcatca ggcctttgcc aacgaggggc ccatccgccg ctggcgtcgg tttggggcca
     3061 gggtgcactt caagccgatt cgcaagcggc aggcgcacgt cacccttgtg ttgcgcgacc
     3121 gcggcgaacc gaccacctag cagcagaacc gacgtgatga agggtatggg gacggattga
     3181 ggagcaagca tggggcgtaa agtccatcca atcggtttca ggctgggggt gaaccggagt
     3241 tgggattcac gttggatcgc aaaacggccg gcctatgtcg ctcagctcaa cgaagaccgg
     3301 ctgattcgag aagtggtcca taagaccact ccccgggccg gcatctccca ggtcgagatc
     3361 gagcggttcg cgaacgcggt ccacgtcacg gttcacacgt ccaagcccgg gatcgtcatc
     3421 ggccgcaagg gggccaacgt caaggagctg cggacgaaat tacaggaagc caccggaacc
     3481 agcatcaagc tggacatcgc cgagatcaag gaaccggacc tggatgccca gctggtcgca
     3541 gaaaacgtcg ccaatcagct cgaacgccgc atcagtcatc gtcgggccat gcagcgggca
     3601 gtagacagcg cgatgcgggc cggcggcaag ggaataaaag tcatgtgtgc cgggcggttg
     3661 gcggggagtg aaatggctcg ccgcgattgg gtccgagagg gccgggttcc ggcacagacc
     3721 ctgcgagccg atatcgattt cgcccgggcg gaggctatga cgacctacgg tcggatcgga
     3781 gtaaaagtgt ggatctatcg gggcgagatc ctgccggagg ccgaaggcca gcccgagacc
     3841 atggacgtat acgtaagcga gtgagggcaa ccagatgttg atgcccaagc gcctaaagtg
     3901 gcgaaagcaa atgcggggcc ggatgaaggg caccgccagc cggggcacca gggtcgaatt
     3961 cggagagtac gggctccagt cgatggaacc gggttggatt tcagcccgtc agatcgaagc
     4021 cgcccgccgg gcattggtgc gctacatgaa gcgcagaggc aaggtgtgga tccgaatctt
     4081 ccccgacaaa ccggtcaccc aacgcgcggc ggagacccgt atggggaagg gcaagggacc
     4141 cgtcgatcac tgggtggcgg tcgtgcagcc gggccggatc ttattcgagg tttccggcct
     4201 ggaccgtgcc gccgcgcagc aagcgatgcg gctggcctcg cacaagcttt cggtgaagac
     4261 caaattcgcc gctcgcgtcg aatccgtgtc cgcggagtag ccagctccct atcccagccg
     4321 agcccgtagc catgaaggcg aaagagttga gagagttgac tgtcgagcag gtggccgaga
     4381 aacttgaagc ggcgcgggag gcctatttca agttgcggtt tcagttctcc accggccaac
     4441 tccccaacac cgcccagctg caggtcgctc gtcgggaagt tgcccgtatt acaacggtat
     4501 tgcaggaaat ggagcgcgca agcgaatgag gaacttaagg cggcgactga ccggggaagt
     4561 tgtgcgggcc gcgatgcaga agacgattac ggtgcgggta gaccgcacct tccgacaccc
     4621 gctgtaccag aaggtgctgc gccgcagcac ccagtatcta gttcacgatg agctgggctg
     4681 cctgcccggc gatcaggtaa agattgtgga gacccgcccg atttcccggc gcaaacgctg
     4741 gatggtggaa gaaatcctgc ggcgaggctc gccggtggag atggttgagt gattcagcaa
     4801 gagagccgga tcaatatcgc cgacaatacc ggcggacgca cgatggaagt gatccgggta
     4861 ctaggcggtt ccaagcgtcg ctacgggcgg gtgggagacc tggtggttgg cacaatcaag
     4921 tccgccgtac caaacagtgc ggtgcgcaag tcggagatcg tgaaggcggt gatcgtccgc
     4981 acggccaaag aataccggcg agcagacggt tcctatatcc gcttcgatga caatgcggcc
     5041 gtggtgctgg acgccgacaa agtcaatccg aagggaaccc gcattttcgg cccggtggct
     5101 agggaattgc gcgagcgggg ttttatgaag atcatctcgc tcgccccgga agtgatctag
     5161 cgagctcgag gatgaaagtc aaactgaaac gaggcgatac ggtggaggtc atcagcggcc
     5221 gcggaaaagg gacccggggc gaagtgattc gagtggtccc cgacggccgg ggcatcgtgg
     5281 tgcgcggggt caacctgcgc aagaagcatc agcgccagat tcaggccgcc ggccggtcga
     5341 tgtcacccgg aatcattcag ttcgaagcgc cgatcaacgt gtccaatgtc atgttggtct
     5401 gcccgaagtg tgggaagctg acccggctgg caatccaccg aacggacggc caggcccaac
     5461 gcttttgcaa gaaatgcgaa aaccaggtcg acggttaggc cgatggacca ctacttgcag
     5521 aaacggtacc gagaagtgat tgtccccaga ctgatgcaag acttcgggct tgccaatcag
     5581 atggaagtcc cgcgcctgcg caagatcgta gtcaacgtcg gagcgggaga agcgatggac
     5641 aatgcaaagg cgatggatgc tgcggttgca gacctttcgg cgatcaccgg ccagcatcca
     5701 attgtcactc gagccaagaa aagcatcgcc aacttcaagt tgcgggaggg gcattcgatt
     5761 ggggccaagg taaccctgcg gggcgaacgg atgtggtcgt tttttgatcg tttggtcaat
     5821 gtggccttgc cacgcacccg agactttcgc gggctctctc cgaacagctt tgatggtcga
     5881 ggcaactaca cgctggggat gcgcgagcag ctggttttcc ccgagattga gtacgataag
     5941 atcgacaagc tgcgtggatt tgaagtgacc gtggttacca gtgcgccgaa cgacgagcag
     6001 ggccggcgcc tgttgaccct gctggggatg ccgtttcaaa ggacggaaag caatggctaa
     6061 gaaatgtatg ccgtaccgcg aattgcgccg caagtacaag gtgagggttc gcaaccgctg
     6121 tcatatttgc ggccgtcccc gcggctacta tcgccgattc gacctgtgcc ggatttgttt
     6181 ccgcgagcag gcgctgcggg ggaacatccc gggcgtggtg aaggcatcgt ggtaggccgg
     6241 atccacctag agaaggagaa gctctacccg gagcacgagc aatgaccatc agcgatccga
     6301 tttccgacat gctaacccgg gtgcgcaacg gcgcgctggc tggccagcag cttgtgtcga
     6361 tgcccagctc caaactcaaa ctggagattt cccggatcct gaaggaagaa ggctacatta
     6421 tgagcttcga gagcgcccag gagaacgacc accccatcct gcggatccga ttgaagtatg
     6481 tcggggagcg ccgggcgcgg cgacccgtga tcaccggcct gcggagaatc agccggcccg
     6541 gcagtcggac ctacgtcagc cggtacaaga tcccctgggt ccggtcgggg atggggatcg
     6601 ccattctgac cactcccaaa ggcgtgatga ccggtattcg ggcgcggcaa ctcggcgtcg
     6661 gcggcgagtt gctctgtgaa gtgtggtgag gcggagaaac tgagccatgt cgcgaattgg
     6721 tcggtcgccg gtagctatcc ccgagggggt tgaagtccgg atcgagggcc agcgggtccg
     6781 ggtcaaggga cccaaagggg agctcgagca ggtgtttcga gatgagatgg ggattaccga
     6841 agaccagggc gtgctgcatg tgacccgcaa ttcggaccag ccgagtgtca gagccctgca
     6901 tgggatgacc cgggcgctgc tgaacaacat gatcctgggc accacaaagg gtttcacgaa
     6961 gaccctcgag atccaggggg tcggctaccg ggctgagctc aagggctcga cgctgatgct
     7021 gtacgtaggc ttctcccatt cggtcgaagt caccccgccc cccgggatta agttcgaagt
     7081 cgaagccaag ggagacgtcg tcaggatctt gggctatgac aagcagttgg tggggcaggt
     7141 ggcggccgac atccggaacg tccgtccgcc cgaaccctat aaaggcaagg gaattcgata
     7201 tcatggcgag cacgtccggc gtaaggcggg caaggccggg aaggtgaccc aataacccat
     7261 ggctagcgca actcgggcag tagcccgcca gcgcaggcag cgcagggttc gggcaaaggt
     7321 gagcgggacg ccccgggtcc cgcggctcaa cgtcttccgc agtctggacc agatctatgc
     7381 ccagttgatt gacgacagcc gcggccatac tttggtttcg gcctcaactg tggatcccga
     7441 gcttcgcccc aaggcggccg gactgcgaaa actcgaacag gccaagttgg tgggagagtt
     7501 gctcgcccag cgagccaagg caatcgggat ggatcgggcg gtgtttgacc ggggcggaaa
     7561 ccggtaccac ggccgggtga aggcggtcgc cg
//
LOCUS       DATDPQ010000103         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2138800, whole genome shotgun sequence.
ACCESSION   DATDPQ010000103 DATDPQ010000000
VERSION     DATDPQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138800"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="amrB"
                     /locus_tag="VI520_02395"
     CDS             <1..1303
                     /gene="amrB"
                     /locus_tag="VI520_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HLF36764.1"
                     /translation="QVDGAPLGLIVPHAGYRYSGATAAYGFKQLSGAQIRTAVVIAAD
                     HQPPLSQPISVWARGGFETPLGVIPVDEQTAERLLGHDTRIQFDPKSHALEHPIEIEL
                     PFLKRVLPDCRIVPILMMSDADDATVELLSEALASALPETGAVVIASSDLSHYPSYDD
                     AVRTDRATLAAIELGEPGLVRQAIRRSMAAGVRSLATCACGEAPILVTMKVAQARGAT
                     HTTLLDYSNSGDAEVDLRSQVVGYGAVMFWRHQPPSLDRAQQQVLLRIARSAAEAQVR
                     MKPANEAAPEDPALRRLSAVFVTLRVRGELRGCIGQLRADTELHQAVAEKATAAASAD
                     PRFSALREEELADLTVEISVLSPLQRVGDPADIEAGIHGLAIFDQGKKGVLLPKVATE
                     RGWGRRQLLENTCLKAGLPADCWKGEAAVYRFETLEIGETG"
     gene            1309..2271
                     /locus_tag="VI520_02400"
     CDS             1309..2271
                     /locus_tag="VI520_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HLF36765.1"
                     /translation="MDWVLLAGLMLILALIVGLWLNANSRMTAITYRLIESGKLQEGT
                     VESFGRIQQSMGALTEAGRKMEAVAEEVAGLSDLLKVPKVRGGVGELLLGDLLAQMLA
                     PEQFRINHSFRSGERVDAVIKLQAGLVPVDAKFPLESFQRAIAGEDEAVRSKHKREFA
                     RALKGHINDIARKYILPDEGTFDFALMYIPAENVYYETIIRDEALGDEGGILTHALEK
                     HVIPVSPNSFFAYLQAIALGFRGLQIEKRAKEIMGFLDRLAGDLARFRADYEVLGGHL
                     DNARKKYDEADKRLGRFEEKLGGVRDPDSQLPGAETPRIGSDPD"
BASE COUNT          472 a          785 c          854 g          460 t
ORIGIN      
        1 gcaggtggat ggggcgccgc tgggcctgat tgtccctcac gccggctatc gctactccgg
       61 ggcgactgca gcctacggat tcaagcagct gagcggggct cagattcgca ccgcggtggt
      121 catcgcggcc gatcatcagc cgccgctctc tcagccgatt tcggtctggg cccggggcgg
      181 gtttgagacc ccgctggggg ttatcccagt cgacgagcag accgccgaac ggctgctcgg
      241 gcacgatacc cggatccaat tcgaccccaa gagccatgcg ctggagcatc ccattgagat
      301 cgaactcccc ttcctgaaac gcgtgctgcc tgactgccgg atcgttccga ttctgatgat
      361 gagcgatgcg gatgatgcga ccgttgagct gctcagcgag gccctggcct cagccctgcc
      421 ggagactggc gcagtcgtga tcgcgagctc cgacctgtct cattatcctt cctatgatga
      481 tgcagtccgg acggatcggg cgaccctggc agcgatcgag ctcggcgaac ctgggttggt
      541 gaggcaggcg attcggcggt caatggcggc tggggtacgc agcctcgcca cctgcgcctg
      601 cggcgaggct ccaattctgg tgacgatgaa ggtggcccag gcccgcgggg ctacccacac
      661 cacattgctc gactactcca actcggggga cgccgaggtc gatctgcgga gtcaggtcgt
      721 cggctacggg gcggtgatgt tctggcgcca ccagccaccc agcctagatc gggcacagca
      781 gcaggtgctg ctgcggatcg cccggagcgc ggccgaggcc caggtgagaa tgaagccggc
      841 caacgaggct gcgcccgagg atcccgccct gcgacgattg agtgcggtat tcgtcaccct
      901 ccgagtgcgc ggcgagctgc ggggttgcat tggtcaactg agggccgata ccgagctcca
      961 ccaggcggtc gccgagaagg ccaccgcggc agcctcggcc gacccacgat tctctgcgct
     1021 ccgtgaagag gaactcgcgg acctgaccgt ggaaatttct gtcttgtccc cgctgcagcg
     1081 ggtgggcgat ccagcggaca ttgaggccgg cattcacggc ctagccatct tcgatcaggg
     1141 gaaaaaggga gtcctgctgc cgaaggtagc gaccgagcgc ggctgggggc gccgccagct
     1201 cctggaaaac acctgtctga aggccggcct gccggcggat tgctggaagg gagaagcggc
     1261 agtgtatcgg ttcgagaccc tcgaaattgg ggagaccgga tagtcagcat ggattgggtc
     1321 ctgctcgcgg gcctcatgct cattctggcc ttgatcgtgg ggctgtggct aaatgcaaat
     1381 tcccgcatga ccgcgatcac ctacaggttg atcgagagcg gcaagctgca agaagggacg
     1441 gtcgagtcat tcgggcggat ccagcagagc atgggcgcgc tgaccgaagc cggccggaag
     1501 atggaagcgg tcgccgaaga ggtcgccggc ctcagcgatc tattgaaggt tccgaaagtg
     1561 cggggcggag tgggggaact gctgctcggc gatctgctgg cccagatgct ggcgcccgaa
     1621 caattccgga tcaatcacag cttccgctcg ggcgaacggg tcgacgcggt cattaagctg
     1681 caggccgggc tggtgccggt ggatgctaag tttccgctgg agagcttcca gcgggcgatt
     1741 gccggcgagg atgaggcggt gcgatccaaa cacaaacggg agttcgcccg agcactcaaa
     1801 ggacacatca acgacatcgc tcggaaatac atcctgcccg atgaggggac cttcgatttc
     1861 gccctcatgt atatcccggc tgaaaacgtg tactacgaga ccatcatcag ggacgaggcg
     1921 ctcggcgatg agggtggaat cctcacccac gctctcgaga agcacgtgat tccggtctca
     1981 cccaacagct tcttcgccta cttgcaagcg atcgcgcttg gcttccgcgg actgcaaatc
     2041 gagaagcggg ccaaagagat catgggcttc ctcgaccggc tggcgggaga cttggcccgc
     2101 ttccgggccg attatgaggt cctcggtggt cacctcgaca acgcccgcaa gaagtacgac
     2161 gaggccgaca aacgccttgg ccgatttgaa gagaagctgg gcggcgtgcg ggatcctgac
     2221 tctcaactcc cgggtgctga aactcctcga atcggaagcg accccgacta ggggattcgt
     2281 cacgcctaat gcagcgagcc gatcgagctc gattgagcac gcatcgtggg gggccttcct
     2341 tgggtgcggc ctacggccgg gcttcgctcg cgaggcttga cacacccctt ggctccccgt
     2401 aggatgggcg cagcccgtcc gcggacgtag cccctatgcg cgcccttgtc gccctggcct
     2461 tgatcctcgc cctgctggtt acggtttttg cggtccaaaa caatgccgat accacggtga
     2521 agttcctggc ctggagcgtg cggggatcgg ccgcgctcgt gctgatgatc a
//
LOCUS       DATDPQ010000104         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_818766, whole genome shotgun sequence.
ACCESSION   DATDPQ010000104 DATDPQ010000000
VERSION     DATDPQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818766"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..>2241)
                     /locus_tag="VI520_02405"
     CDS             complement(154..>2241)
                     /locus_tag="VI520_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HLF36766.1"
                     /translation="LLEYIPTAVSQREIREAIRKAGFRTLEVEETLEDPEGEARAKAV
                     RHQWRLFIRGAVLTAPVLSMAMLADFGVIPPAISGAAWFGWILLALTTPVVFHVGWQF
                     LEGSYKAIRNRTANMDVHVAMGVLAAYGYSLAVALGWVEGQPFFESAAVIVTLIVLGR
                     YLEARAKSQTSDAIRALLRLKPKLARIERGGRELEVPVDEVIVGDLVVVRPGEQLPVD
                     GVVVDGHSSVDESMLTGESIPVSKRAGEAVFAATLNRLGMLKFEATKVGRDTALAQII
                     RLVQGAQASKAPIQHLADKVSSVFVPAVLPVAALTFIAWFFFVPPSPELSQFTRALIS
                     TVAVLVIACPCAMGLATPTAVTVGLGKGAQMGILFRSSDALERTGRISTFVLDKTGTI
                     TRGQPAVTKIQSDGAGIEDAELLRMAASAEMGSEHPIGEAILAEAQARGLRPNPPDRF
                     EAFPGGGVLAHVAGQTVLVGNAKLLQEQGVSTDGLADQLMQLEAAAETAVGVAVDGKL
                     AGILAVADQLKEGSRQAVQELQQMGLRVMLMSGDNRRTASAIGRQVGLFDSEDVLSEV
                     RPAEKAEQVRRLQANGIQIAMVGDGINDAPALAQASVGIAIGTGTDVAIAAAPVTLMS
                     GDLRVLPRAIRLSRITLRVIKQNLFWAFFYNTLLIPLAALGQLSPMLAAAAMAFSSIA
                     VVTNSLRLRRMRI"
BASE COUNT          458 a          738 c          660 g          385 t
ORIGIN      
        1 ggcgagccgg aacagaccag gccggtcggc cacggctaag ccggagaggg agaccccggc
       61 caaccggata aagtctcttc tggaaaactt gcccatgccg cgaaggaggt tacctgaaca
      121 aaggggctgg gacaaaggat ccgaccatgt ggctcagatc cgcattcggc gcagccgaag
      181 gctattggtg acgaccgcga tcgaactgaa agccattgcg gcggcggcca acatcggact
      241 cagctgcccg agggctgcca gcgggatcaa gagggtattg tagaagaagg cccagaacag
      301 gttttgcttg atcacacgta gggtgatccg cgaaaggcgg atcgcgcgcg gcaatactcg
      361 caggtctccg ctcattaggg tcaccggcgc ggcggcgatg gccacgtcgg tccctgtgcc
      421 aatcgcgatt ccaacgctcg cctgtgccag tgcgggtgcg tcgttgatcc cgtcgccgac
      481 catcgcaatc tgtatcccgt tcgcttgcaa acgcctgact tgttcggcct tctcggctgg
      541 acgcacttca gaaaggacat cctccgagtc gaacagccca acctgtcggc cgatcgccga
      601 ggcggtccgt cgattgtcgc cactcatcaa catgacccgt agacccatct gctgcagttc
      661 ctgcacggcc tgccgggatc cctccttcaa ttggtcggca accgccagaa ttccggccaa
      721 tttgccatca acggcgaccc ccaccgcagt ctcagccgcc gcctcgagct gcataagctg
      781 atctgccaga ccgtctgtcg aaactccctg ctcctgaaga agcttggcat tcccaacgag
      841 cacggtctgc cccgcgacat gagcgagcac ccccccgccc ggaaatgctt cgaaccgatc
      901 cggcgggttt ggccgcaagc cacgcgcctg agcctctgcg aggatggcct ctccaattgg
      961 gtgctcagag cccatctcag cgcttgcggc catgcggagc agttccgcgt cctcgatccc
     1021 agctccatcg gactggatct tggtgaccgc gggctgccca cgggtgattg tgccggtctt
     1081 atcgagcacg aaggttgaaa tccgtccggt ccgctcgagc gcatcgctcg accggaacaa
     1141 gatccccatc tgggcgccct tcccgagtcc gactgtgact gccgtcggag tcgcaaggcc
     1201 catcgcgcag gggcaggcga tcacgaggac cgccaccgtg ctgattaggg cccgggtgaa
     1261 ctgggagagt tcggggctgg gtggcacgaa gaaaaaccag gcgatgaaag tcaaggctgc
     1321 gaccggcagg accgccggaa caaacacgga agatactttg tccgccaggt gctggatcgg
     1381 ggccttgctg gcctgggccc cttgcaccag gcgaataatt tgtgccagag cggtatcccg
     1441 tcccactttt gtggcctcga atttcagcat ccccaaccgg ttcaaggtcg cggcaaacac
     1501 cgcctcccct gcccgcttgg agaccgggat cgattcaccg gtcagcatcg actcgtcgac
     1561 cgaggagtga ccatcaacga ccaccccatc gaccggcagc tgttcgcctg gacgcacgac
     1621 cactaaatcg ccgacaatca cttcgtcaac ggggacctca agctcgcggc cccctcgctc
     1681 aatgcgggcc agcttcggct tgagccgcag caaggctcga atggcgtcgc tggtttggga
     1741 tttggcccga gcctccaggt aacggccgag cacgatcaag gtaacgatga ctgccgcgga
     1801 ctcgaagaac ggttgcccct cgacccagcc aagagccact gcaagtgagt atccgtaagc
     1861 cgcaagcact cccatcgcaa catgaacgtc catattggcc gtccgattgc gaatggcctt
     1921 gtaactgcct tccaggaact gccaacccac atgaaagacc accggtgtcg tcagggccaa
     1981 caggatccag ccaaaccacg cagcaccgga aatcgcgggc gggatcacgc cgaagtcggc
     2041 gagcattgcc atcgacaaga caggcgctgt gaggaccgca ccccggataa acagcctcca
     2101 ctggtggcgg acggccttgg cacgggcttc tccctcgggg tcctccagcg tctcttcaac
     2161 ctcgagggtc cggaagccgg ctttgcggat cgcctcccga atctcgcgct gcgaaacggc
     2221 ggttggaatg tactcaagca g
//
LOCUS       DATDPQ010000105         3993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3436472, whole genome shotgun sequence.
ACCESSION   DATDPQ010000105 DATDPQ010000000
VERSION     DATDPQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3436472"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VI520_02410"
     CDS             complement(<1..671)
                     /locus_tag="VI520_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLF36767.1"
                     /translation="MKRILSIDGGGVRGIIPLACLIQLEAQEGKPCKEIFNMIAGTST
                     GAVIAAGLAIGISARGLLALYRNLAESAFQALPFWKIAANLGNHRYSNEFISRTLSEI
                     GADRELNSLPVDILITGKNTATSRTDFFVRDNPGNASLWGRLSLRDSVLASIAAPTYF
                     PAHSAELGGVQHTWVDGGVGVAGNPCYQAAVEAIHYSAGAYPPGDTRLLSFGTGSSPH
                     PVNPKR"
     gene            complement(668..1408)
                     /gene="lipB"
                     /locus_tag="VI520_02415"
     CDS             complement(668..1408)
                     /gene="lipB"
                     /locus_tag="VI520_02415"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HLF36768.1"
                     /translation="MNGCEVHSLGILPFREAYSLQARMAEEIAEGVRPQSLLLLEHPH
                     TFTFGRRAKPENLVWGRPELASRSLEVHWTDRGGDVTYHGPGQLVGYPLLRLGRLDPT
                     GRLPQADYVGYVRRLEQVLIRAVATLGLASGQRRGLTGVWVEPEVASRCPSCPPAARR
                     APSKLASIGIRVDAQGISRHGFALNVAPDMSYWQGIIACDLPGASMISLAELLDPAPA
                     MSEAAQAVVVEFGRAFGLEMRPVEEAIL"
     gene            1711..>3993
                     /locus_tag="VI520_02420"
     CDS             1711..>3993
                     /locus_tag="VI520_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HLF36769.1"
                     /translation="MPSMICLQRLRRAHRSRRDSQRGLLLGFLAISLSAAALAGLAAG
                     STLLGLAAELPEVARIEGQFGLRGAEAFRPLLLFDRTGEVVLFQNLPPAAVDRIWVDP
                     PQAPQHVVQATVAALDPTFWTNPGYELRGLADGSSLTITQRVTAAALLPPAAEDSSPA
                     GRTLQNVLLAAELTRRYPKERILAWYLNSADYGRSAYGIDAAGLVYFGKHAGELSPAE
                     SSMLAALALDPELDPVDSPQQARSAQAAVLDEMVAAGDLSRAEADRAANQPLQLLSAS
                     MNWRPPDFAAYALQQVRSVLGRSILSRSGVRVISSLDHDLQQQAACAAESHAERLRGN
                     PAGTVVPATDGTPCVSAALLPTLRPSDVGLDHQIDGWAVVVTDPQRGEILAASGPFGQ
                     PSPAGPVLSPFLYLTAFSRGSSPSAMVIDLGPPLEGAQGPIRMRTALAGLLPGPEATL
                     QAGVGEDSLLRSLGQLGLQVLPGEDGQLRSQQVDLFDLAKAYGILAADGRSRGLQLAD
                     GSIVPTIILRVEESDGVQLYVFEPSEQAIVSSQLAYLTVAVLSDEPARWSSLGSPNQL
                     EIGRPAAALAGSSPGQEANWAIGFTPDRVVGVLLSGAPLRQVDRLNGAASLWHAIIRF
                     ASAGLPAQGWQLPAGLSEIEVCDPSGLLPTIYCPEVVREIFIQGSEPTHYDNLYQPFR
                     VNAETGKLATIFTPLASIEERVYFVPPPEAAQWARAVGLERPPEEFDALPSAPLFSPG
                     ARILSPGAFEIVGAEVPILGE"
BASE COUNT          678 a         1257 c         1327 g          731 t
ORIGIN      
        1 cgcttgggat tgaccgggtg cggcgagctg ccggtcccga acgagagcaa tcgggtgtcg
       61 cctggcgggt atgcgccggc gctgtagtgg atcgcctcca ccgcggcttg atagcatggg
      121 ttgccggcga cgcccacccc accatccacc caggtgtgct gcacgcctcc gagctcggcg
      181 ctgtgggcgg gaaagtaggt tggcgcggca atgctggcaa ggacgctgtc tctcaaggac
      241 aaccggcccc aaagcgaggc gttgccgggg ttgtcccgca cgaagaagtc agtccggctg
      301 gtggcagtat ttttgccggt gatcaggata tcgaccggca ggctgttcag ctcgcggtcg
      361 gcaccgatct cgctgagggt ccgtgagatg aactcgttgc tgtagcggtg gttgccgaga
      421 ttggcggcga tcttccagaa cggcagcgcc tgaaacgcgg actcggccag gttgcggtag
      481 agcgcaagca gcccccgggc gctgatcccg atcgccaacc cagcggcaat tacggcgccg
      541 gtcgaggtcc cggcgatcat gttgaagatc tccttacagg gcttaccttc ctgggcctcg
      601 agttggatca ggcaggccag cgggatgatc cctcggactc cgcctccgtc gatggagaga
      661 atcctcttca taggatcgcc tcttcgaccg gacgcatctc gagtccaaaa gcgcggccga
      721 actccactac gacggcttgg gccgcctccg acattgcggg ggccgggtcc agcaactcgg
      781 cgagcgagat catggaggca ccgggaagat cgcaggcgat gatcccttgc cagtagctca
      841 tgtccggcgc aacgttcagg gcaaagccgt gccggctaat cccctgggcg tcgacccgga
      901 tcccgatcga ggcgagcttg gaaggagccc gcctggcagc gggcgggcag cttgggcagc
      961 gtgaggcgac ctccggctct acccacaccc cagtcaggcc gcggcgttgg ccgctggcca
     1021 gcccgagggt cgccacggcc cggatgagga cctgctccaa ccgacgaaca tagccaacgt
     1081 agtcggcttg ggggaggcga ccggtcgggt ccagcctccc caatctgagc agcggatatc
     1141 caaccagctg accgggaccg tgataagtca catcgccgcc gcggtcagtc cagtgcactt
     1201 ccaggctgcg ggaggcgagt tcgggccggc cccagaccag attctcgggc ttggctcgcc
     1261 ggccgaaagt gaaggtgtgg ggatgttcta ggagcagcag gctctgtggg cggacgccct
     1321 cggcgatctc ctcggccatc cgagcttgca gggagtatgc ctctcgaaag gggaggatcc
     1381 caagcgaatg gacttcacag ccattcacca ctaacctcgc cgaggatcag ccatgcttgg
     1441 gttcgtgcac atctgaaccg agggtccccc aacgcccacc ttcctcaaag aactagtcta
     1501 tcaccggctg agccggggag gtctgcggca cgctgagctc gatgccggca tagatggtcg
     1561 cctaacagtc gcaggggaag ttggcatcgg ggtgcaggtc ggggatcagc agggatgcta
     1621 cccgctctgc ttggctgaga tgctcatggg tctggctgag cagtggattt gcacgcctgg
     1681 cctcaatcgg agatgattag ccggcggatt atgccctcca tgatctgtct gcagcgatta
     1741 cgccgggccc accgttcgcg gcgggattcc caacgcggac tactgctcgg tttcctggcg
     1801 atcagcttgt cggccgccgc gctggccggc ctggctgctg gatcgacact gctcgggctg
     1861 gccgctgagc tgcccgaggt cgcccggatc gagggtcaat tcggtctgcg gggggccgaa
     1921 gccttccggc cgttgctctt gttcgaccgg accggtgagg tcgtgttgtt tcagaatctg
     1981 cctccggccg cggttgatcg gatctgggtg gatccgccgc aggctccgca gcatgtggtg
     2041 caggccaccg tggctgcact ggatccgacc ttctggacta atcccggcta cgagctccgg
     2101 gggctggcgg atggttcaag cctgaccatc acccagcggg tcaccgcggc agccctgctc
     2161 ccgccggctg ccgaagactc ctcgccggct ggccgcaccc tgcagaatgt gctgttggcg
     2221 gccgagctca cccgccgcta tcccaaagag cggatcttgg cctggtacct gaacagcgcc
     2281 gactatggcc ggtcggccta cggtatcgat gcggccgggt tggtgtattt cggaaagcat
     2341 gctggcgaac tcagcccggc agagagctcc atgctggccg ccttggcgct cgatccggaa
     2401 ctcgacccgg tcgattcacc acagcaggcc agatcggcac aagcggccgt gttggacgag
     2461 atggtggctg ccggcgatct atcgcgggcg gaggccgatc gcgccgcgaa ccaacccctg
     2521 cagctcctat ccgcttccat gaactggcgt ccgccagatt ttgcggcgta cgcgttacag
     2581 caggtcaggt cggtgctcgg gcgcagcatt ctgtcaagaa gcggggtgcg ggtgatctcc
     2641 agcctggatc atgatctcca gcagcaggcg gcctgcgcag ccgagtcaca cgccgagcgg
     2701 ttgcggggga acccggctgg gacagtcgtt ccagcgacgg acggtacgcc ctgcgtctcg
     2761 gccgccctct tgccgaccct aagaccgagt gacgttgggc tcgatcacca gatcgatggc
     2821 tgggcggtgg tggtgactga tcctcagcga ggggaaatct tggcggcctc cggtccgttt
     2881 ggccagccca gtccggcggg tccggttctc agcccatttc tctatctgac cgcgttctca
     2941 cgaggcagct ccccttctgc gatggtgatc gacttgggcc cacccctgga gggggctcag
     3001 ggaccaattc ggatgaggac cgccctcgcc ggtctacttc caggtccgga ggcgaccctg
     3061 caggctgggg tgggagaaga cagcctgctg cgcagtcttg gccaactcgg gctgcaggtc
     3121 ttgcctggcg aggacggaca actgcggagc cagcaagtag atctattcga cctggccaag
     3181 gcttacggca tcctggccgc cgatggacgc agccgcggac tgcagctcgc agacggttcg
     3241 atcgtgccga cgattattct acgggtggaa gagagcgacg gggtacagct ctatgttttc
     3301 gagccgagcg agcaagccat cgtcagctcc cagcttgctt atctcacagt cgccgtactg
     3361 agcgatgagc cggcccgctg gtcgagtctc ggttcgccga accagctgga gatcggtcgg
     3421 ccggccgccg ccttggcggg cagctcgcca ggccaggagg ccaattgggc gatcggcttc
     3481 accccggatc gggttgtggg agttctactg tccggagcgc cgcttcgaca ggtcgatcga
     3541 ctcaatgggg cggcgagcct ctggcacgcg attatccgat ttgcatcggc cgggcttcca
     3601 gcccagggct ggcaactacc ggcggggctc agtgaaatcg aagtctgcga tccatccgga
     3661 ttactgccca cgatctactg ccctgaagtg gtaagggaga tctttatcca gggcagcgag
     3721 ccgactcact atgacaacct gtaccaaccc ttccgagtca atgccgaaac cgggaagctg
     3781 gctaccatct tcactccact agcctcgatc gaagagcgag tgtacttcgt ccctccgccg
     3841 gaagcggcgc aatgggcccg agcggttggg ctggagcggc cgcccgaaga attcgatgct
     3901 ctgccgagtg ccccgttgtt ttcccccgga gcccggattc tctccccagg cgcattcgaa
     3961 atcgtggggg cggaagtgcc gatcttgggt gag
//
LOCUS       DATDPQ010000106         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3703997, whole genome shotgun sequence.
ACCESSION   DATDPQ010000106 DATDPQ010000000
VERSION     DATDPQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3703997"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VI520_02425"
     CDS             complement(<1..868)
                     /locus_tag="VI520_02425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF36770.1"
                     /translation="MSIQSAAVKTIRAGEEQVVVTVGACPLCLSTQAEPFESVSEGAK
                     TVSYRLCADCGLVYQSPRMSDAGLQQYYQAEYVAQHQRATGVSEKELRVQTGRARNLV
                     RLLRPQAESVSRHLDIGCSAGLLLEAVRGAYGCESVGVEPAEVYRNYCAARGLTAYSD
                     LDRLAEADNDRFDLVSLAHVLEHLTDPVGYLADLRNTWLRSGSKLLVEVPNLFGHTSL
                     ETPHLACFHSGTLRRTLAVAGYRVIRLIRHGEPRSRLIPLYLTAIAEPAAGMVSRESV
                     RFGAGGVRRRRRF"
     gene            complement(868..1815)
                     /locus_tag="VI520_02430"
     CDS             complement(868..1815)
                     /locus_tag="VI520_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36771.1"
                     /translation="MRGVTLFSAPKPFVEPHIGLIQINAASSWMALGPEVEVLWIGDE
                     PGLAKAAAALGVRQLAPVGRNTSGTPLVSSVFELAQQAAHHDLLGYVNADILLLDDFV
                     KSVDAVANRFHRFLIVGQRWDLAIGSKLAFTGDWSSDIRKRLGQEGHLHPLAGSDYFV
                     FPRGSLGRMPEFAIGRAGWDNWMIYAARRMGLPVIDASQAITAVHQNHDYAHLPNGRP
                     HHGLPESEQNVRLAGGRQTIFTLADATWQIAGGEIEKIRWPRGGVLRALEAAITSRAG
                     SAGLLRVVRILFHPLEFLRSRSGIAARWIRSRIGARGDA"
     gene            complement(1833..2603)
                     /locus_tag="VI520_02435"
     CDS             complement(1833..2603)
                     /locus_tag="VI520_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLF36772.1"
                     /translation="MDRRRVVAIVQARMASSRLPGKVLADIGGQPMLVRVLERARRAS
                     RLALVVVATSTEKDDDAVVALCADRAYDCYRGDPLDVLDRYVQTARRFEAEVIVRLTG
                     DCPLIDPEVIDRTVAAFLEANPPVDFAANRLPAERTFPIGLDTEVCSRTALELAGREA
                     AARHHREHVMPFFYENPNRFRTLLVHNDVDYGHLRWTVDTQEDLVLVREIYAQFAGKD
                     HFSWMEVLDLYERQPRLAEINASVAHKTELDLDPRWNP"
     gene            complement(2606..>3065)
                     /locus_tag="VI520_02440"
     CDS             complement(2606..>3065)
                     /locus_tag="VI520_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF36773.1"
                     /translation="LLLLGSGSLETEIQNRIALGGAEHQVHFAGQVEFARLPEMYRAA
                     DLYVSASHSDGSSVSLLEAMACGLPALVSDIPGNREWVEPEVNGWWFRDRSGKDLARL
                     LRHAVSGAADLPAMGRSSRRVAETRADWSVNSQELMRAYQLAATDGRAVG"
BASE COUNT          568 a         1054 c          915 g          528 t
ORIGIN      
        1 caaaccggcg ccgccgccga acgcctccgg ccccgaaccg gaccgattca cgggagacca
       61 tcccggccgc gggctcggcg atcgcggtca ggtacaaggg gattagacgc gaccgaggtt
      121 caccgtgccg gatcaatcga atgacccggt agccggccac tgccaatgtc cgccgcagcg
      181 tgccgctgtg gaagcacgcc aggtgcggcg tctcgagcga ggtgtggccg aacagattgg
      241 gaacctcgac caggagtttc gagcctgacc gcagccatgt attccgaagg tccgcaaggt
      301 acccgaccgg gtcggtcagg tgctccaata cgtgggccag gctcaccagg tcgaagcggt
      361 cattgtccgc ttcagccagc cggtccaagt cagagtaagc cgtaaggccc ctcgccgcac
      421 agtaattgcg gtagacctcg gccggttcaa cgccgacgct ctcgcagcca taagccccac
      481 gcacagcctc caacagcaat ccagccgaac atccgatgtc gaggtggcgg gacaccgatt
      541 ccgcctgcgg ccgcagcaat cgcacgagat tgcgggcccg cccggtttgc accctcagct
      601 ctttctcgct caccccggtt gcccgctgat gctgcgcgac atactcggcc tggtagtact
      661 gctgcagccc ggcatcagac attcgggggg actgatagac cagcccgcag tccgcgcaca
      721 ggcggtagga cacggtctta gctccttcgg agacgctctc aaagggttcg gcctgcgtgc
      781 tcaggcagag cgggcaggca ccgactgtta ctacgacctg ttcctctccg gctctgatcg
      841 tcttgaccgc agcactctga atcgacacta cgcgtctcct cgtgccccga tccgggaccg
      901 gatccagcgc gcggcaatcc cgctgcggct ccgcaagaat tccagtgggt ggaagagaat
      961 ccgaacaacc cgcagcaggc ccgccgaccc agcccgggag gtgatcgcag cctccagcgc
     1021 ccgcagaacc ccgcccctcg gccatcggat cttctcgatc tccccgcccg cgatctgcca
     1081 ggtcgcatcc gctaatgtga agatcgtttg acgtccgccg gccagccgca cgttctgctc
     1141 gctttcgggc agtccatggt gcggtcttcc gttcggcaaa tgagcgtagt catggttttg
     1201 gtggacggcg gtgatggcct gactggcgtc aatgaccggc agccccattc gacgggcggc
     1261 gtagatcatc cagttgtccc atcccgcccg cccgatggcg aattcaggca tcctgcctag
     1321 cgatccgcgg ggaaacacga aatagtcgct cccagcgagt gggtgaaggt gaccctcctg
     1381 cccgagccgc tttcgaatgt cgctgctcca atctcccgtg aaggccagct tcgagccgat
     1441 cgcaaggtcc cagcgttggc ccacgatcag gaatcggtga aaccggtttg cgactgcatc
     1501 caccgatttc acaaaatcat ccaggagcag gatgtctgcg ttgacatacc caagcaagtc
     1561 gtggtgggcg gcctgttggg ctaactcaaa cacagagctc accaacgggg tgccactggt
     1621 attccgcccg accggcgcca gttgcctgac tccgagcgcg gctgccgcct tcgccagccc
     1681 cggctcgtct ccaatccaca acacctccac ctcaggtccc agggccatcc aggaactcgc
     1741 cgcattgatc tgaatcaacc cgatgtgggg ttccacgaag ggcttggggg ccgagaacag
     1801 agtcactccg cgcatgggtt cgagcgccgg gcctatggat tccagcgggg atccagatcg
     1861 agctcagtct tgtgagcgac cgatgcattg atttccgcca gccggggctg tcgctcgtag
     1921 agatcgagca cttccatcca agaaaagtgg tccttcccgg cgaattgcgc atagatctcc
     1981 ctaaccagga cgaggtcctc ctgggtgtct acggtccagc gcaggtggcc atagtccacg
     2041 tcgttgtgga ccagcagggt acgaaatcga ttcgggttct cgtaaaagaa gggcatcacg
     2101 tgctctcgat gatggcgagc ggcagcctcc cgcccggcga gttcgagggc cgtccggctg
     2161 cacacttcgg tgtccaaacc aataggaaag gtccgctcgg cgggaagccg gttggccgcg
     2221 aagtcgaccg gcggatttgc ttccaggaag gccgccacgg tccggtcaat cacttccgga
     2281 tcgatcagcg gacagtcgcc ggtgaggcgc acgatgacct ctgcctcgaa tctgcgggcg
     2341 gtctggacat atcggtccag cacatccagc ggatctcccc ggtaacagtc gtaggcccga
     2401 tctgcgcaca gcgccacgac agcatcgtcg tctttctcgg tgctggtggc cacgacgacc
     2461 aaggccagac ggctcgcccg gcgggcccgc tccagcaccc ggaccagcat cggctggccg
     2521 ccaatatcgg ccaggacctt gcctggcagc cggctggaag ccatccgagc ctgcacgatc
     2581 gcgacaaccc gccggcggtc catgttcagc cgaccgccct tccatcggtt gcagccaact
     2641 ggtacgctcg catcaactct tgggagttga ccgaccaatc cgcccgggtt tccgcgactc
     2701 gccgcgagct gcgccccatg gccggtaggt ctgctgctcc gctcacggcg tgccgcagca
     2761 gacgggcgag gtcttttccg ctcctgtccc gaaaccacca accattgacc tccggctcca
     2821 cccactcccg attgcccggg atgtcggaga ccagcgccgg caggccgcag gccatcgcct
     2881 ccagcaacga aaccgaactg ccatcgctgt gggaggcact cacatagaga tcggccgccc
     2941 ggtacatctc cggcagacgg gcgaactcca cctggccggc gaaatgcacc tggtgttcgg
     3001 cgccccctag cgcgatccgg ttctggatct cggtctccag gcttcccgag cccagcagga
     3061 ggagc
//
LOCUS       DATDPQ010000107         4987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3437366, whole genome shotgun sequence.
ACCESSION   DATDPQ010000107 DATDPQ010000000
VERSION     DATDPQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437366"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VI520_02445"
     CDS             <1..349
                     /locus_tag="VI520_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36774.1"
                     /translation="EPEMIDLVRLILGRRGFEVVGANGGLEGLALVASEKPDLVLLDL
                     MMPDMDGWEVYQRMKSDEALRDIPVVVVTAKAQSIDKVLGLHIAKVDDYITKPFGPQE
                     LLESVEKILGPID"
     gene            452..1825
                     /locus_tag="VI520_02450"
     CDS             452..1825
                     /locus_tag="VI520_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLF36775.1"
                     /translation="MSWGRRLGLLAVCLIILWPARAMAQAEGPTYIVQDGDTLFGIAL
                     TFGSTVAELAAANGIEDPSAIFPGQELLIPGLTGILGTLQFEPLAFGETMASQARRHG
                     LPLEQLARLNRSLNPQSLIVGQPFVSSAQELPSLALPTSERWVVQPGDSLLELAVRLN
                     VDVWTLQALNRLEARRWSVQGEMQYAAVGDQPTTGLPPDLEAIEVRPLPVVQGQVLVV
                     QLSTSSGSIPSGHLGPWNLQFIEDAPSHWIALQGIHALQDAGVLELEIGLAQAEPFSQ
                     SIELEAGEYGREALQVPPETLDPANTGPEDELIAGVVAEVTAEKLWEGPFSFPASFFE
                     AFPSVFGTRRSYNGSDFTFYHTGLDLYGSTTTPVLAPAAGRVAYASFLTVRGNTTYID
                     HGWGVFSGFLHQSQILVEPGDQVEQGQIVGYVGGTGRVTGPHLHWEVWVGGVPVEPLI
                     WTRSSFP"
     gene            1944..3536
                     /locus_tag="VI520_02455"
     CDS             1944..3536
                     /locus_tag="VI520_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36776.1"
                     /translation="MRSISPPGSESYWAEFELNEADLTFIDNLLLERGIPLTPKEMAE
                     VIVRDRLDRERVAREQSKRPVAQPYLPAQRYLVGQQLQFQNLANGVGTVTRVRGSSNP
                     ALPPFEVIQVELGDELREFAAGFPDHVLNSIEQAPTDMDRGLSPDAITQRSGSRIAQE
                     IDASLGKRPEVVRIAGKWFHRGLLAEINEGHLNLAEAVLDVAAGGPLPTQDLVKHMET
                     SPGLDPLLTEFSVDYALQEDERFDEVGPAGKVLWFLKRLEPPEVLQPPERLVDQSTPY
                     DRSVLGPELLTLERELDDELSVGSPPADPPAPEVILVLPFPHWSIGSLPLSARLQAMF
                     PTAYEAPRIRFILVDGHSGAKFPGWVVRTQRYVFGLADWFRQYDVPAGGLIRVRPGRE
                     DGEVLVEAVDPRRRNDWIRTATVGDGGRIGLTMLKHPVGTAYDERRIVGVIDRRALEQ
                     AWQNTSQRTLPLDRLVPQMFRELSKLNPQTTVHAEALYAVVNVVRRIPPGPILAELVN
                     RPYFEPVGDHYWRLNEDEWKKA"
     gene            3521..3973
                     /locus_tag="VI520_02460"
     CDS             3521..3973
                     /locus_tag="VI520_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36777.1"
                     /translation="MEKGVTTAAAKIPTSRVRPTLSTQFHIDREWWERAGRELQVYLR
                     DHLCLEHRQEYDESGPGLKVDHVDPYSGEVTVMDRFQDLLISHCSQQPDFVTPQMSLI
                     NAIFRIFLASGNLPQTPEQLSERTGRSAHTILRTLSGPRIYRGIRPLE"
     gene            4018..4090
                     /locus_tag="VI520_02465"
     tRNA            4018..4090
                     /locus_tag="VI520_02465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4051..4053,aa:Arg,seq:tct)
     gene            complement(4112..4336)
                     /locus_tag="VI520_02470"
     CDS             complement(4112..4336)
                     /locus_tag="VI520_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36778.1"
                     /translation="MNIELKSSNSDLTEAVQSAVEAIRRGDLGQGRATLTWVLRENPS
                     DRLAWLWMACCVTDEPERDECYRQASKIAI"
     gene            complement(4430..>4987)
                     /locus_tag="VI520_02475"
     CDS             complement(4430..>4987)
                     /locus_tag="VI520_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HLF36779.1"
                     /translation="NLLREGLDLPEVSLVAILDADKEGFLRSSTALIQTIGRAARHVE
                     GTVIMYADKMTDSMRAAISETDRRRAKQLRHNQEHSIEPASIVKAVRDLTDQVAIHAV
                     GEARGEYRVMAPAHLPKDELSRLIKELQKEMQAAAESLEFEKAAALRDQIFEFRQTLA
                     EMENLPPWQRVRLLAGEEPRREPEL"
BASE COUNT          925 a         1544 c         1515 g         1003 t
ORIGIN      
        1 cgaacctgag atgattgatc tggtccgctt gatccttggg cgcaggggtt ttgaagtcgt
       61 aggcgccaac ggggggctgg agggactcgc cctggtggcg agcgagaaac cggacctcgt
      121 cctgctggac ctgatgatgc ctgatatgga cggctgggaa gtctatcagc gaatgaaatc
      181 cgacgaggca ctccgcgaca tcccggtggt cgtcgtcacc gccaaggctc agtcgatcga
      241 caaagtgctt gggctgcata ttgccaaagt ggatgactac attaccaaac ctttcggtcc
      301 gcaggagttg ctcgagagcg tagagaagat cctcggtccg atcgactaag caatcgctga
      361 aaccatcgat tcacaacggc ggggccaagc tggccccgcc gtttattgcc tggcgggacg
      421 tctcgaatcg ggccagggct ataatctggc catgtcctgg ggtcgccgcc tcggcctgct
      481 agccgtgtgc ctgattatcc tctggccggc ccgggcgatg gcgcaagcgg aaggcccgac
      541 ttacatcgtg caggacggag acaccctctt tgggatcgcg ctaacctttg gatcgacagt
      601 ggccgagctc gccgcggcca atggaattga ggatccttcc gccattttcc caggccagga
      661 gctgttgatt ccgggcctga ccggcatcct gggaaccctc cagtttgaac ctctggcgtt
      721 cggtgagacc atggccagcc aggcgcgccg ccacgggtta ccccttgaac agctggcccg
      781 gctaaatcgt tccctcaacc cgcagagctt aatcgtaggt cagccgttcg tctcatcggc
      841 ccaggaactc ccgagcctcg cgctgccgac ttccgagcgc tgggtggtcc agccgggtga
      901 ctcgctcctg gagctggcgg tgagactcaa tgtggatgta tggactcttc aggcactcaa
      961 ccggttggag gctcggcgct ggtcggtgca aggcgagatg caatacgcgg ccgtggggga
     1021 ccaacccacc actggcctgc cgccggactt ggaggcaatc gaggtcaggc cgctacccgt
     1081 agtgcagggt caagtcctgg tggtgcaact gagcacgtcc tccgggtcga taccgtcggg
     1141 acatctcggc ccttggaatc tgcagttcat cgaagacgcc ccttcccact ggatcgcgct
     1201 gcaggggatc catgctttgc aagatgccgg ggtgctcgaa ctcgagatcg gcctggccca
     1261 agctgagccc ttcagtcaat ccattgagct cgaagccggt gaatacggcc gggaggctct
     1321 gcaggtcccg ccggagaccc tcgacccagc gaataccgga cccgaggacg agctgattgc
     1381 aggagtcgtc gccgaggtta cagccgagaa gctctgggag ggcccatttt ccttccccgc
     1441 cagctttttc gaggccttcc cctccgtgtt cggcacccgc cgaagttaca acgggagcga
     1501 tttcaccttt taccacacgg gtcttgacct atatggcagc accaccactc cggtcttagc
     1561 ccctgcggct ggacgggttg cctatgcaag tttcctaaca gttcgtggca acacgaccta
     1621 cattgaccac ggttgggggg tcttcagcgg attcctgcat cagtcgcaaa tcctcgtgga
     1681 gcccggggat caggtggagc agggccagat cgttggttat gtcggtggaa ccggccgggt
     1741 gaccgggccg caccttcact gggaagtgtg ggttggggga gtcccggtcg agccactgat
     1801 atggacccgc agctctttcc cataggatta gagtccaaca gtcctcgccc gcggtctgcc
     1861 caacagccga tcggccgttt agtctggtca atcagatcgg cgtagtataa tttccgcccg
     1921 ctcccctcga gccctatccc cgaatgcgat ccatctcccc gcccggaagt gaaagctact
     1981 gggcagagtt cgaactcaac gaggcggacc tcactttcat cgacaacttg ctgctcgagc
     2041 gaggaatccc tctgacgccc aaggagatgg cggaggtgat tgtccgcgat cggctcgatc
     2101 gtgagcgggt ggcgagagag caatccaagc ggcctgtcgc ccagccgtac ctcccggcac
     2161 agcgctatct cgttggccag cagctccagt ttcagaactt ggctaatggg gtaggcactg
     2221 tcacccgcgt gcggggaagc tcgaatcccg ctctgccgcc gttcgaggtc attcaggtag
     2281 aacttggcga tgagctgcgg gagttcgcag cgggatttcc agatcacgtg ctgaactcaa
     2341 ttgagcaggc acccacggac atggacagag gactgagtcc ggacgccatt acccaaagga
     2401 gcggcagccg aattgcccag gaaatcgacg caagcttggg caagcgaccc gaagtggtcc
     2461 ggatcgcggg gaaatggttc caccgcggtc tgctggctga gatcaacgaa ggccacctca
     2521 acctggcaga agccgtgctc gacgttgcgg cgggtggccc gctgcccacc caggatctgg
     2581 tcaagcacat ggaaacctcg ccgggtctcg accccctgct gaccgaattc tcggtcgact
     2641 acgccttgca ggaagatgag cgatttgacg aagtcggtcc ggccggtaaa gtcttgtggt
     2701 ttctgaagcg gcttgaaccc ccagaggtct tgcagcctcc ggagcggctc gtggatcaga
     2761 gtacccctta tgatcggtcc gtgctcggcc cggagctgct tacattggag cgggagctcg
     2821 atgatgaact cagcgtgggc agcccgcccg cagatccacc agctccggag gtcatcctgg
     2881 tgctgccgtt tcctcattgg tcaatcggct cgttgccact gtccgcccgc ctgcaggcca
     2941 tgttcccgac tgcctacgag gccccccgca tccgtttcat tctggtcgac ggccacagcg
     3001 gagccaaatt cccaggttgg gtcgttcgaa cccagcgtta cgtattcggt ctggcggatt
     3061 ggttccggca gtacgatgtg cctgcggggg ggctgatccg ggtgcggccg ggtcgggaag
     3121 atggcgaagt gctggtcgag gcggtcgatc cacgtcggcg gaatgattgg atccggaccg
     3181 ctaccgtcgg ggatggcggc cggattggtc tgacgatgct gaagcatccg gtgggtacgg
     3241 cctatgatga gcgaaggatc gttggggtca tcgaccgacg ggcgctcgag caggcttggc
     3301 agaacacctc gcaacgcacc ctgccactcg atcggctcgt gccccaaatg tttcgggagc
     3361 tctcgaagct caacccgcag actacggtgc atgccgaggc tctgtatgcc gtagttaatg
     3421 tagtcagacg aattccgcca gggccgatcc tggcggaact ggtgaatcgt ccatacttcg
     3481 agccggtcgg cgatcactat tggcgtttga acgaagacga atggaaaaag gcgtgacgac
     3541 cgcggcggcc aagattccga cctcgcgggt ccggccgacc ctcagcactc aattccacat
     3601 tgaccgcgag tggtgggaac gggccggccg tgaactccag gtctacttgc gtgatcatct
     3661 gtgcctggag caccggcagg agtatgacga atcaggaccc ggcttgaagg tggaccatgt
     3721 ggacccctac agcggcgagg tcactgtcat ggaccgattt caggacctac tgatctcgca
     3781 ctgctcccaa cagccagact tcgttacccc ccagatgagc ctgatcaacg cgatttttcg
     3841 aatctttctt gccagtggca acctccccca gacaccggag cagctctccg aacgtaccgg
     3901 ccggtctgct cacacgatcc tgcgcacgct ctccgggccg cggatctaca gaggaatccg
     3961 tcccctcgaa taatatgaga taatctgctt cgcctcgggt ttactaacct cgtcctagcc
     4021 cctgtagctc aatggacaga gcgccggcct tctaagccgt aggttgcagg ttcgaatcct
     4081 gccgggggcg cctcgtttgg cgaagcgctc ctcatatagc gatcttcgaa gcctggcggt
     4141 agcattcgtc gcgctccggc tcatcggtca cgcaacaagc catccacagc cacgccaggc
     4201 ggtcactggg gttctcccgt agcacccagg tcagcgtcgc tcgaccctgg cccaaatcac
     4261 cacgccgaat agcttcgacc gcggattgca ccgcctccgt cagatctgaa ttgctgctct
     4321 tcaattcgat gttcatttgc gacctcgcag aaaccgttga gcagcgccct catgttctcc
     4381 ctctgattca tcgtagcact ccgacccgcc agccgggcta acggctagct tacagttccg
     4441 gctcccgccg tggctcctcg ccggctagca gccgcactcg ctgccaaggt gggaggttct
     4501 ccatttcggc gagggtctga cggaattcga agatctggtc gcgcaatgcg gcagccttct
     4561 caaactccag agactcggcc gcggcctgca tttctttctg gagctctttg atcaagcggg
     4621 agagctcgtc tttgggcagg tgggcgggag ccatgactcg atattcgccg cgggcttcgc
     4681 ctacggcgtg gatggcgact tgatcggtca ggtcgcgaac cgccttcacg atgctggctg
     4741 gctcgatgct gtgctcctgg ttatggcgga gctgcttggc ccgccgccga tccgtctcgc
     4801 tgatggcggc ccgcatcgaa tcggtcatct tgtcggcgta catgatgacc gttccttcca
     4861 catgccgggc cgcccggccg atcgtctgga tcagggcggt gctggaacgc agaaaaccct
     4921 ctttgtccgc gtcgaggatc gccaccaagg agacctccgg cagatcgagc ccctctcgca
     4981 gcaagtt
//
LOCUS       DATDPQ010000108         3792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1093460, whole genome shotgun sequence.
ACCESSION   DATDPQ010000108 DATDPQ010000000
VERSION     DATDPQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093460"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VI520_02480"
     CDS             complement(<1..759)
                     /locus_tag="VI520_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HLF36780.1"
                     /translation="MTGPLVRPGPLTHGTELNAVYAIARIVAETYDTEAGLDAVFRLS
                     RSIFIFDVVSLFLTNEETGQLEPAYALALGRGRSREADLAWGEPAAIEAYRSGQTILR
                     QEDAGAAVQGRDRRRDYLGLPMMVHGRCVGGLVFGRFGGPSYPPEHIRLAEFVAWHVG
                     QLLENRRMARRIATLEAQRELARMQDEFISTISHELRTPLGFIKGYVTTLMRKDATWE
                     PETRMEFLQIVDEEADRLRELIDNLLDSSRLETGT"
     gene            complement(756..1835)
                     /locus_tag="VI520_02485"
     CDS             complement(756..1835)
                     /locus_tag="VI520_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36781.1"
                     /translation="MDLALLALLAERAYLAGVPETAPLTDTPPAFIPRLGDYLVRRGL
                     LTTGQLESALVHQKELAKSGERRLLGQTLVELEMVDRETIDRAITSQIIELHAALQEA
                     NRNLEKRVSERTAELRQALGRLQELNTLKTNLISNVSHELRTPLAHVKGYVELLEEQE
                     LGKLTPEQAKAVSVISRATLRLEHLIEDLIAFSTASREGLALDLRPAQLAQLAEAVLE
                     RSKDKAALEGVAVQIAVDRGLPPVQADLDKLSWVLYQLVDNGIKFTPAGGRVKLWAQQ
                     VDRFVTVAVSDTGIGIPSDKLEEIFEPFHQLDGSPTRRHGGTGLGLALVKQIVSAHGA
                     SIAVESAENSGSTFRFDLPLATEPA"
     gene            2064..2459
                     /locus_tag="VI520_02490"
     CDS             2064..2459
                     /locus_tag="VI520_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36782.1"
                     /translation="MAIEVLLIDDDPDLSTMLRTLLRGQDFQIKAVFSSEDGISKVQN
                     PDSTPDVVILDLLMPEVDGWEVCERIRSFSDVPILILSALGSPGSVARALDAGADDYL
                     IKPVHASLLASRLRTLVRRKALNPNIQAA"
     gene            complement(2542..2826)
                     /locus_tag="VI520_02495"
     CDS             complement(2542..2826)
                     /locus_tag="VI520_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HLF36783.1"
                     /translation="MNVVALTLELHLPGCSSLKEKRGRLKPVLTALHREFNVSAAEID
                     HQDAHRTAVIACVVVSNSNRHGEQLLSGIPVWLEQLRPDFQIVDHQLTVL"
     gene            complement(2823..3638)
                     /locus_tag="VI520_02500"
     CDS             complement(2823..3638)
                     /locus_tag="VI520_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HLF36784.1"
                     /translation="MPRKRWRMISSPPATGAENMALDQAILERVAAGESPATLRLYAW
                     APPCLSLGHAQSIELVDLRSLGELGWNLVRRPTGGKAILHTDELTYSVSGIAGDPLFA
                     GGVLASYQRLSAGLFHGLELLGLGAALRLSPNRSPPITGSSPICFEVPGPFELTWAGK
                     KILGSAQLRRSGAVLQHGSLPLAGDLGRICLVLHLPDREQAQLAVRRSAATVEQALGQ
                     QISWESAARALAAGFREALELDLIEGQPTPAEIQRSQELSAERFSIMEEAVHP"
BASE COUNT          714 a         1172 c         1190 g          716 t
ORIGIN      
        1 ggttccggtc tccagtcgcg aagaatcgag caagttgtcg atgagctctc ggagccggtc
       61 agcttcctca tcgacgatct gcaagaactc catccgagtt tcaggttccc aggtggcgtc
      121 tttgcgcatc agggtcgtca catagccttt aatgaagccg agcggggtac gcagctcgtg
      181 cgagatcgtt gaaatgaact cgtcttgcat gcgggccagc tcacgctggg cttccagggt
      241 cgcgatccgc cgagccatac gccgattctc caacagctgc ccgacgtgcc aggcgacgaa
      301 ctcggccagc cggatgtgtt ccggtggata cgaggggccg ccaaatcggc cgaaaaccag
      361 cccgccgacg cagcggccgt gcaccatcat cggtaagccc aggtaatccc gacggcggtc
      421 ccggccttga accgcggcgc cggcatcctc ttggcgcaga atcgtttggc cgcttcggta
      481 agcctcaatg gcagccggct cgccccaggc caggtcggcc tcccgcgatc ggccgcggcc
      541 aagcgccagc gcgtaagccg gctcgagctg accggtctct tcattggtga gaaatagtga
      601 aaccacgtcg aagatgaaaa tcgatcggga aagccgaaac accgcgtcga ggccggcttc
      661 agtgtcgtag gtctcggcca cgatccgcgc gatcgcatag actgcgttta actccgtgcc
      721 gtgggtcaat ggccccgggc gaaccagcgg tcctgtcatg caggctccgt ggcaagtggg
      781 agatcgaagc ggaaggtgct tccggagttc tccgcacttt ccaccgcgat cgaggcccca
      841 tgggcgctca cgatctgttt gaccaacgcg agcccaagcc cggtaccccc atggcgacgg
      901 gtcggcgatc cgtcaagttg atggaaaggc tcaaagatct cctccagttt gtccgagggg
      961 atgccaatcc cggtgtcgga gacggcgacc gtaacgaagc gatcaacctg ttgcgcccag
     1021 agcttcaccc gccctccggc cggggtgaat ttgattccat tgtcgaccaa ctggtaaagg
     1081 acccaactga gcttgtccag atcggcctgg acggggggca gacctcggtc gaccgcgatc
     1141 tgaaccgcga cgccctcaag ggcggcctta tccttcgatc tttcgagcac tgcctcggcg
     1201 agctgagcaa gctgggccgg ccggagatcc aaagccagcc cttcccggga agccgtggag
     1261 aaagcgatca aatcctcaat caagtgctca agccgcaggg tggcccgact gatcaccgag
     1321 accgctttgg cttgctccgg ggtaagcttg ccgagttcct gttcttccaa caactcaaca
     1381 tagcccttca catgcgctag tggggttcgc aactcgtgcg agacgttcga gatcaagttg
     1441 gtcttaaggg tattcaattc ctgaagccgc ccgagcgctt gacgcagttc ggcggtccgc
     1501 tcggacaccc gcttttcgag gttccggttg gcctcttgca gagcggcatg gagctcgatg
     1561 atctggctgg tgattgctcg gtcgatcgtc tcccgatcga ccatctccag ttccaccaag
     1621 gtctgcccaa gcaagcgacg ttccccgctc ttggcgagct ccttctggtg gaccagagcc
     1681 gactcgagtt ggccagttgt cagcaagcct ctgcgaacca ggtaatcgcc gagccgggga
     1741 atgaaggccg gcggcgtgtc ggtcagcggg gcggtctctg ggacgccggc cagataggct
     1801 cgttcggcga gcagtgcgag cagggccaga tccaccccgc aactggggca agtcctggaa
     1861 tcccccggca tgataaagga gcactgcggg caggcgatgg agctggttac gaccggggat
     1921 tcagacgtgc tcaaagcggg ctcgcgggta tattagccgc tccagggaag agcggcaagt
     1981 ggaaggtagt actgcaaggt ctctgtaagg ttcggggccc accttcctgt cggataatgt
     2041 tgccgatgca ctaggagcgc aagatggcga tcgaagttct cttgattgac gacgacccgg
     2101 acctctccac gatgctgcgc actctcttgc gcgggcagga cttccagatc aaggcggtgt
     2161 tctcgagcga agacggtatt tcgaaggtcc aaaatccgga ttcgacccct gatgtggtca
     2221 ttttggacct gctgatgccc gaagtggacg gttgggaagt ctgcgagcga attcggtcgt
     2281 tctcggacgt tccgatcctg attctatcgg ccctcggttc gcccggaagc gtcgcccggg
     2341 ccttggacgc gggagcggac gactatctga tcaagccggt ccatgccagc ctgctcgcct
     2401 ctcggctgcg gacgttggtt cggcgtaagg ctctgaatcc aaacatccag gctgcctgag
     2461 caccgaccct acaacagcag caggaagcca cgcagcgttt gctgcgtggt tgtgttttag
     2521 gcgccggatc atgcagccgg gttagagtac ggttagctgg tggtccacta tctggaagtc
     2581 aggccgaagc tgctcgagcc agactggaat gccggacagc agctgttcgc catgccgatt
     2641 gctgttgctc accacgacgc aggcgatcac tgcggtgcgg tgagcgtctt gatggtcgat
     2701 ttcggcagcg gacacgttga attcccggtg cagggcggtg agcaccggct tcagccggcc
     2761 ccgcttttcc tttaaagagc tgcagcccgg gagatgaagc tctagggtga gcgccacaac
     2821 gttcatggat ggactgcctc ttccattatc gaaaaccgct cggctgacag ctcctggctg
     2881 cgctggattt cagccggagt cggctggcct tcgatcaggt cgagctccag tgcttctcga
     2941 aatccagcgg ccaaggcccg agcagctgac tcccagctga tctgctggcc gagcgcctgc
     3001 tcgaccgttg cggccgaccg gcggaccgct aactgcgcct gttctcggtc gggtaggtgg
     3061 agtaccaaac aaatcctgcc aaggtcgccc gccaagggaa gcgagccgtg ctgcagcacg
     3121 gcacccgacc ggcgcagctg ggcggacccg agaatcttct tgccggccca ggtaagttcg
     3181 aaggggcccg gcacctcaaa acaaatcgga ctggaaccgg tgatgggggg agaccggttc
     3241 gggctcaatc gcagcgcagc ccccagcccg agcagctcga gtccgtggaa gagacccgcg
     3301 ctgagccttt ggtagctcgc cagcacccca ccggcgaaga gcggatcgcc ggcaatcccg
     3361 gacaccgaat acgtgagttc atccgtgtgc aggatggcct ttcctccggt cggacggcgg
     3421 accaggttcc agcccagctc gcctaagctc cggagatcga ccaattcaat ggactgagcg
     3481 tgtcccaggg agaggcaggg cggagcccag gcgtagagcc gtaaggtagc cggagactcg
     3541 cccgccgcga ccctctccaa gatcgcctgg tccagcgcca tgttctctgc tccggtggcc
     3601 ggcggggagc tgatcatgcg ccagcgcttt cgaggcatgc gcgggattat acctagcgga
     3661 ccggtatggt agactgcccg cgaactcgcc cccggccgcc ccctatgacc agtgtgaaat
     3721 cctcatcctc gctattgtgg cgcggctcgc tgctctgcct tgcgctgctg accgggtgta
     3781 ccgcgatcgg ac
//
LOCUS       DATDPQ010000109         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2143075, whole genome shotgun sequence.
ACCESSION   DATDPQ010000109 DATDPQ010000000
VERSION     DATDPQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143075"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VI520_02505"
     CDS             complement(<1..1149)
                     /locus_tag="VI520_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLF36785.1"
                     /translation="MRIESLLAARLFLSPQRARDRLYFISNLSGKLSLYAMDTGGSVP
                     EPLLPPNIALQNPTLMTGYSYYLLPELDKLLVMIDANGDENYQPMLVPMSGGIPEPAF
                     GEQFRGYRVHLEKCYPADGIAYFAAESRSAQLVEAFQARLGPAWSLTRLAGSPWGELG
                     HRSGYVNAVNTEHSKVLLREGYTLGDNLLRLWEKGKAEPALVYGKPLEERGPGESVPL
                     AGIFNCHFTKGDRGLLFVTALFEDHFGLGFLGLDGEQAIRPVRVGEPRHTGYGELYKL
                     DHLQDDRFVIQYNIDGCSWGYEGTFDEARLEMKLKQVLWGQGELSQGVLEADHYDQHS
                     DSYSLAFSTATSPAQIYTLDGPNRDRLAAHTRERVLGIPQQWMARGEDA"
     gene            complement(1215..2153)
                     /locus_tag="VI520_02510"
     CDS             complement(1215..2153)
                     /locus_tag="VI520_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36786.1"
                     /translation="MSRIALLLLFTAACSRGEQASSGPEVMAGESPAPVSASGGPALN
                     ESEVVGGDEAALREFIGRALTYHYAGATGGTTVLVGGLPENLGFDLPVPEDSRVIGSI
                     VRGTLEGGTEIILDVDLAPEEALAFYREQLLQAGWEEQTEQAYGGGFVSGSWPSTTFC
                     LNENDAVIFLSAYAPPSLPTELRLNIQQEAQYSACLAQPMGMDEASGLIPSLVSPPDS
                     LIQSGGGSSGEGMAETSAVLRSNLSTGALAEQYAAQLLEAGWKPLAQDEEPELAWSFW
                     SVADAEGQDWSGLLLIFKSPIVPEKVFASFRVDRGD"
     gene            2274..>2584
                     /locus_tag="VI520_02515"
     CDS             2274..>2584
                     /locus_tag="VI520_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HLF36787.1"
                     /translation="MKTLFNKSAVEGDHEVGTQLQQSGYRLTQPRKAVIRALTEATDW
                     LRPEDLLALARRYWRPLGLVTVYRTVSLLAELGYLRRVHLEDRCVGYVRSELVHGHHL
                     L"
BASE COUNT          480 a          852 c          744 g          508 t
ORIGIN      
        1 agcgtcctct cccctggcca tccattgctg cggaatcccg aggacccgct cccgggtatg
       61 ggctgccagt cgatcgcggt tgggtccatc gagggtgtag atctgagccg gcgacgtggc
      121 ggttgaaaag gccagtgaat aactgtctga gtgctggtcg tagtgatcgg cctccagcac
      181 accctggctt agttcgccct gcccccacag cacttgcttg agcttcatct ccagccgggc
      241 ctcgtcgaag gtcccttcgt agccccagga acacccgtcg atgttgtact ggatcacgaa
      301 gcgatcgtcc tgcaggtggt ccagcttgta gagctcaccg tagccggtat ggcgaggctc
      361 gccgaccctc accggccgga tcgcttgttc gccatccagg cccagaaacc ccagaccaaa
      421 gtgatcctca aagagggcgg tcacgaacaa cagtccgcgg tcccctttgg tgaaatggca
      481 gttgaagatt ccggcaagcg ggaccgattc ccccgggcct cgctcttcga gcggtttccc
      541 gtacaccaag gccggctcgg cttttccctt ctcccacaga cgcagcaaat tgtcgccgag
      601 cgtgtagccc tctcgcagga gcaccttgga gtgctccgtg tttacagcat ttacatagcc
      661 ggatcggtgg ccgagttccc cccatggact gcccgccaaa cgggtgaggc tccaagctgg
      721 cccaagccgg gcttgaaagg cttcgacgag ctgcgcactt ctggattcgg ccgcgaagta
      781 ggcaatcccg tccgctgggt agcacttctc caggtggact cgatagcccc ggaactgttc
      841 cccaaaggcc ggctctggaa tcccacctga catcgggacg agcatgggct gatagttctc
      901 gtctccgttg gcgtcgatca tcaccagcag cttgtccaac tccggcaata ggtagtacga
      961 gtagcccgtc atcaaggtcg ggttttgcag cgcgatgttc gggggaagca gcggctcggg
     1021 cacgctgccg ccggtgtcca tggcatacag gctgagcttg cccgacaagt tgctgatgaa
     1081 gtacagccgg tctcgggcgc gctggggact cagaaagagg cgggcggcca acagcgactc
     1141 gattctcacg attggtctct ccacgtagta ttttggtggg ctcggcccgg gtggcctctg
     1201 gcgaaccgga ttggctagtc cccgcggtca accctgaatg aagcgaagac tttttcgggc
     1261 acaatcggac tcttgaaaat caggagcagt ccagaccaat cctgaccttc tgcgtccgca
     1321 acactccaaa aactccaggc cagctctggt tcctcatcct gcgcaagcgg cttccaccct
     1381 gcctccagca actgcgccgc gtactgctcg gccagagcgc ctgtggaaag gttgcttctg
     1441 agcacagcgg aagtttccgc cataccctcg ccactgctcc cacccccaga ctgaatgagc
     1501 gaatccggcg gggatacgag cgaggggatc aggcccgagg cctcatccat gcccatcggc
     1561 tgagcgagac aggcggagta ctgtgcctcc tgctggatat tcagtcgaag ctcggtgggc
     1621 aggctcggcg gggcgtacgc tgacaggaag atgactgcgt cgttctcatt caggcaaaag
     1681 gtcgtgctgg gccaggaccc cgaaacaaag cctcccccgt aggcctgctc tgtttgctcc
     1741 tcccaacccg cctggagcag ctgctcccga tagaacgcaa gcgcctcctc gggagccaag
     1801 tccacgtcga ggatgatctc ggtgcccccc tccagggttc cgcgcacgat actgcctatc
     1861 acccggctgt cttctggaac cggcagatca aagcctaggt tttccggcag gccgcctacc
     1921 agtaccgtgg tgcctccggt cgcgcccgca taatggtagg tcagcgcgcg accaatgaac
     1981 tcgcgcaacg ccgcctcatc gcctccgaca acctcgctct cattcaacgc agggccaccg
     2041 ctcgctgaga cgggggcagg actctcgcct gccatcactt ccgggccgga tgacgcttgc
     2101 tcgccgcggc tgcaggcggc ggtaaataac aatagcagcg cgattcggct catggcccct
     2161 ccctcaaaat acatgcgcct gatcgctcga ttctaaggcc tattggcagc cccggcatgc
     2221 ctcagcctcg ctgcttgacc gtcgcttgat ccccccttat gatttgcacc taagtgaaaa
     2281 ctcttttcaa taaatcggct gtcgaaggcg accacgaggt cggcacccag ctgcagcaat
     2341 ccgggtatcg gctcacccaa cctcgcaagg cggtcattcg agccctgacg gaggctaccg
     2401 actggctccg gccggaggat ctgctcgccc tcgctcgccg ctactggcgc ccgctcggac
     2461 tggtgacggt ataccgaaca gtctcccttt tggccgagct cggctatctc cgacgggtgc
     2521 acctggaaga tcgctgcgtc gggtatgttc gctccgagct cgtgcacgga catcatctgc
     2581 tctg
//
LOCUS       DATDPQ010000110         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3438022, whole genome shotgun sequence.
ACCESSION   DATDPQ010000110 DATDPQ010000000
VERSION     DATDPQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3438022"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..2543
                     /locus_tag="VI520_02520"
     CDS             177..2543
                     /locus_tag="VI520_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HLF36788.1"
                     /translation="MALLMAALAMAQPASAHAILVRSVPDSNSDLGSPPPTIEMWFSE
                     PLEAAFSSARVIDSSGGEVPTGAAVLDPSDPTHLTLPLGDLGPGIYTVAWQTFSQVDG
                     HEWFGSFPFTVLNPDGTRPSSTAAGVEGGSRGELPSPGEASARWLSLVGGMLMFGTLV
                     FGSVVVPVNRFPVLSVRARQLLVWALWGAAAGIFLGSAAQLLLQAQRLGGLATVPKLI
                     LATRSGAVVLARVLFAATGLNAGLELARPKDGAWRRAVLDIATYAIVVLLLVLLAGTT
                     EQIVALITLAVVVGCLAWTGRSLRRGSVVPETSRIQALTVLAVGILLSFSLSSHAGAA
                     PGSVFAVAGDSLHLLAASAWIGGLLSLPILWLMTRDGTGREEFFPAVQRFSTLAAGAV
                     YIVLLTGVFSALVELPTLRSLWDTPYGVVLLVKLALVILALGIAYLNNRAVRAATHSA
                     VLPRRIAVEAGISALILVTVAFLVQTPTPRSLQVPASPAAARLPFNAIGFTGDLNIHL
                     QVDPNQAGSNRFWTHLFMSDGSSIGEVQLVRLLFEYLDQTLGNSSIDLGPLGQGTFAS
                     EGANLSQVGQWAVDVYVRRRGTDDILARFTLEVAAGQTSAAAPSLFGHPVPGMPALVL
                     VGGLVLSAGLVPFLWRREVKVLAGTSYRAWLRLGGGVVFLGIIALVFGLTTARAAEVP
                     LLERSNPVPATADSLARGEEVYLQACWICHGPAGLGDGPVGVTLNPRPSNLRIHTVPG
                     VHTDGQLFEWVANGFPNSAMPEFSETYSEEDRWNVINYIRTFAAGEVP"
     gene            2540..>2815
                     /locus_tag="VI520_02525"
     CDS             2540..>2815
                     /locus_tag="VI520_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36789.1"
                     /translation="MNLRGMLLAGGFVLAACSTPSSAEGVVGLGLVGRVLYTQGPEGL
                     WQIDMASGEIEQLWKLPEGGQLTGVAVSPDGLDLALSYAPPRGESPFI"
BASE COUNT          436 a          867 c          911 g          601 t
ORIGIN      
        1 ccacctcccc cggaaccgcc ccctctggtc cctcccgaag ggccgctccc taccccgacg
       61 gtaagtccac ctgagggtgg cggttgcgcg ctcagattaa tgccgttaat cggactcatg
      121 ggtctcagat gccttcccag aaaagccaga cgctaagaag agcgaggctc gccctagtgg
      181 ctctgctcat ggctgccttg gccatggcgc agccagccag cgcgcacgcg atcttggttc
      241 gctcggttcc ggattcgaat tcagacttag ggagtccgcc tccgacgatc gagatgtggt
      301 ttagcgaacc cctggaggcc gccttctcgt ctgcacgcgt cattgattcc agcggcggcg
      361 aggttccgac cggggcggca gtcctcgatc cgtcagaccc gactcacctg actttgccgc
      421 tcggagacct cggtcctggc atctatacgg tcgcctggca gaccttctcg caggtcgacg
      481 gacacgagtg gttcggatcc tttccattca cggtgctaaa tccggacggg actcgcccga
      541 gcagcacggc ggccggggtt gaaggtggca gccggggaga attgccatca cctggggagg
      601 ccagcgcgcg ctggttgtcg ttggtcgggg gcatgctaat gtttggcacg ctggtttttg
      661 gctctgtggt cgtaccggtt aatcgtttcc cagtgctgtc agtgcgggcg cggcaattgc
      721 tcgtatgggc gctttgggga gcggcggccg ggatcttcct ggggagcgcg gctcaactgc
      781 tgctccaggc acagcggctc ggtggcctgg caacggtgcc aaagctgata ttggcaactc
      841 gttcgggggc ggtagttcta gctcgcgtcc tgtttgcggc aaccggcttg aacgccggtc
      901 tggagcttgc tcggccgaag gacggcgctt ggcgccgggc ggtgcttgac atcgcgacct
      961 atgcgattgt cgtcctgctt ctggtcctgc tggccggcac caccgagcag atcgtggccc
     1021 tcatcaccct ggccgtcgtc gtgggatgcc tggcttggac cggccggtcg ctccgccgag
     1081 gatcggtagt cccggagact agcaggatcc aggccctgac cgttctggcc gtggggatct
     1141 tgttgagttt ctctttgagc agccacgccg gggcggctcc gggcagcgtc ttcgccgtcg
     1201 caggcgacag cctgcacctg ctggctgcct ctgcctggat cggcgggcta ttatcgctcc
     1261 caatcttgtg gctaatgacc cgggatggca ctggcagaga ggaattcttc cccgcagtcc
     1321 agcgattctc caccttagca gcgggcgccg tttacatcgt cctgttaacc ggcgtcttct
     1381 ctgcgctggt ggagcttccc accctccgat cgctgtggga cacgccctac ggagtcgtac
     1441 tgctcgtgaa actcgcgctc gtcatcctgg cgctgggaat cgcctacctt aacaaccgag
     1501 ccgtgcgggc cgcaacccac tcggccgtcc tgccgcgcag aatagcagtg gaagccggga
     1561 tctccgctct gatcctggtc acggttgcgt ttctagttca gacccccact ccccgcagct
     1621 tgcaggtccc ggcttcgccg gcggcagcca ggttgccatt caatgcaatc gggttcactg
     1681 gcgatctaaa cattcatctc caggttgacc caaatcaggc cggcagcaac cgattctgga
     1741 cccatctttt catgagtgac ggctcctcga tcggagaggt ccagttggtc cggctgcttt
     1801 tcgagtacct cgatcaaacc ctcggaaatt cgagtataga tctgggtccc ctcgggcaag
     1861 gcacgtttgc ctccgaggga gccaatttga gccaggttgg ccaatgggcc gtagacgtct
     1921 atgtcagaag aagggggacg gacgacattt tggcccgctt tactctcgag gtggccgcgg
     1981 gacaaacttc ggcggctgca ccgtcgctct tcgggcaccc ggtgcctggg atgccggcgc
     2041 tggtgcttgt tggaggactg gtgctctccg ccggtctcgt gccatttctc tggcggcgcg
     2101 aagtgaaagt gctggccgga acctcctacc gggcctggct tcggctcggc ggaggagttg
     2161 tgtttctagg catcatcgcc ctggtgttcg gattgacaac ggcgcgggca gccgaggttc
     2221 cgctgctgga gcgcagcaat ccggtcccgg caacggcgga ctcgctcgcc aggggcgagg
     2281 aagtctacct acaggcctgt tggatttgcc atggtccggc tggattgggc gatggcccgg
     2341 tgggagtcac actcaacccg cggccttcga accttcggat ccacacggtt cccggcgtcc
     2401 acaccgatgg acagctgttc gagtgggtgg cgaacggttt tccgaattcc gccatgcccg
     2461 agttttctga gacttactct gaggaggatc gctggaacgt gatcaactac attcgaacct
     2521 tcgctgccgg ggaagtgcca tgaacctacg cggaatgctg ctcgcgggtg gcttcgtgct
     2581 cgccgcctgt tcaacgccat cgtcggctga aggtgtcgtc gggcttggtc tggtgggaag
     2641 agtgctctat acccagggac cggagggcct gtggcagatc gacatggcgt ccggcgagat
     2701 cgagcagctg tggaagctgc cggagggtgg gcagctgacg ggtgtggcgg tgtcgccgga
     2761 tggtctggac ctggcgctat cctacgcccc gccccgaggc gagtcgcctt tcatt
//
LOCUS       DATDPQ010000111         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1618120, whole genome shotgun sequence.
ACCESSION   DATDPQ010000111 DATDPQ010000000
VERSION     DATDPQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618120"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1062)
                     /locus_tag="VI520_02530"
     CDS             complement(13..1062)
                     /locus_tag="VI520_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF36790.1"
                     /translation="MARSPTPSSGHLLVVDDNKVNRLLLGRNLEQQGHSVAFAENGQQ
                     ALDRLRAESFDLVLLDILMPELDGYQVLERLTKDPELRDIPVIMTSSMDEMDSVVKCI
                     EMGAEDYLSKPPNPVLLKARIGASLEKKWLRDQQRELIRKFATAEVAEELLTTGFELG
                     GKYVDATVMFSDIRSFTSIAEAQSPADTIELLNDYYTLMFEAIGGHGGVVNQMVGDGL
                     MAVFGAPVPSADHAERAVLSALEMLEMVGMFNAEQSARQKVQIQIGVGIASGRVIAGF
                     TGTQNRATYTCVGDTVNLAARLEAQTKLAGHPILIDENTQAGLNGKIRSEELEPVQLR
                     GKSQVVRIFAVPPGQ"
     gene            complement(1052..1402)
                     /locus_tag="VI520_02535"
     CDS             complement(1052..1402)
                     /locus_tag="VI520_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLF36791.1"
                     /translation="MQPGLIDQTALDSLLATTDREFVGELIDAYLEDSPGLIAAMLRA
                     LAEANATEFTRAAHSLKSSSASLGAASLSDLAKELEHLGKDSKLTGAASKLEQLAGLF
                     EQVKTALEEFKRGS"
     gene            complement(1409..>3247)
                     /locus_tag="VI520_02540"
     CDS             complement(1409..>3247)
                     /locus_tag="VI520_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36792.1"
                     /translation="FGYTAEEAIGRNLDDLVANLDEIRDEARNYNKHSASGELLRAIA
                     RRTRKDGSLVDVEVLALPVKVGQEQQGVIVIYYDITELVLARQSAEAANEAKSAFLAT
                     MSHEIRTPMNAVIGMSGLLLDTTLNDEQREFAEIIRNSGDSLLTIINDILDFSKIEAG
                     KMDLESQPFDLRECVESALDLVAPRAAEKGLELAYQLDEEVPRGVLGDVTRLRQILIN
                     LLGNAVKFTERGEVVLNVVTKRRAWAHDLHFSIRDTGLGISNEAIGRLFQSFSQADAS
                     TARKHGGTGLGLAISRRLTELMGGKMWAESEPGVGSTFHFTVVTQAAELPQVARPELA
                     AEQPKLVGKRVLIVDDNETNRRILVVQTKSWGMTPLETGSPRLALDWIRRGESFDLAI
                     LDMHMPEMDGLALAKEIRQLRASAELPMVLFTSLGRREADLRGVDFAAQLTKPIKPSQ
                     LFDALIAVFAGAEEPAAAGDGRAKTKIDPEMGKRHPLRILLAEDNAVNQKLALRLLQG
                     MGYRADVAGNGLEAIQSLERQRYDVVLMDVQMPEMDGLEASREINRRWKSEQRPRIVA
                     MTANAMQGDRELCVAAGMDDYIAKPIRIPELVGALSKATARQAEGI"
BASE COUNT          574 a         1052 c          952 g          669 t
ORIGIN      
        1 tgctgctcag gactactggc cggggggtac ggcaaagatt cggaccacct gcgacttgcc
       61 ccgcagctgg accggttcca actcctcgga gcgaatcttt ccgttgagcc cggcttgggt
      121 gttctcgtca atcaggatcg ggtggccggc cagtttggtc tgggcttcca gccgggcagc
      181 tagattgacc gtgtcgccga cgcaggtgta ggtggcgcgg ttctgagtcc cggtgaagcc
      241 ggcgatcact cgcccggagg cgatcccgac cccaatctga atctggactt tctgccgggc
      301 ggactgttcg gcattgaaca ttccgaccat ctccagcatc tccagggcgg acaagactgc
      361 ccgctcggcg tggtcggcgc ttggcaccgg cgccccaaag accgccatca agccatctcc
      421 caccatctga ttgaccactc cgccgtgccc gccgatcgcc tcgaacatca aggtgtagta
      481 gtcattcaac agctcgatgg tgtcggccgg cgattgggcc tcggcgatgc tggtgaagga
      541 gcggatgtcg gagaacatca cggtggcgtc cacgtatttg ccgccgagtt cgaatccggt
      601 ggtcagcaac tcctcggcta cctcggcggt ggcaaacttg cggatcagtt cccgctgctg
      661 atcccgcagc cacttcttct ccaggctggc gccgatgcgg gctttgagca gcaccggatt
      721 cggaggctta gatagatagt cctcggcccc catttcaatg cacttcacca cgctgtccat
      781 ctcgtccatc gaagaagtca tgatgaccgg gatgtcccgc agttcgggat ctttggtgag
      841 ccgctctaaa acctgatagc cgtccagctc gggcatcagg atgtccagaa gtaccaggtc
      901 gaaagactcc gctcgcaacc tgtcgagcgc ctgctgccca ttctcggcaa aggccacgct
      961 gtgtccttgc tgttcgaggt tgcgccccag cagcaggcga ttgactttgt tgtcgtcgac
     1021 aacgagcagg tgaccgctgg aaggcgtcgg gctacgagcc acgcttgaac tcctcgaggg
     1081 ccgtcttgac ttgctcgaag agaccagcca gctgctcgag cttggaggcg gcgccggtca
     1141 gtttcgagtc ctttcccaga tgctcgagtt ccttggcgag atcggatagg ctcgccgcgc
     1201 ccaagctggc gctgctagat tttagcgaat gagcggctcg ggtgaattcg gtcgcattcg
     1261 cctctgccag tgcccggagc atcgcagcga tcaacccggg cgaatcttcc aggtaagcgt
     1321 cgatcaactc gcccacgaat tcccggtcgg tcgtggcgag cagcgagtcc agagctgttt
     1381 ggtcaatcag gccaggctgc atcggcgcct agattccctc cgcctgtcgg gcagtggctt
     1441 tgctgagcgc gccgaccaat tcggggatcc ggatcggctt tgcgatgtaa tcgtccatcc
     1501 ccgccgcaac gcacagctcg cgatcgcctt gcatggcgtt ggcggtcatc gccacgatcc
     1561 gtggccgctg ctcggacttc cagcgccggt tgatctcgcg ggaggcctcc agcccgtcca
     1621 tctccggcat ctggacatcc attaacacca catcgtagcg ttggcgctcc aaagactgaa
     1681 tcgcctccag gccattgccg gccacatccg ctcgatatcc cataccctgc agcagccgca
     1741 gggccagctt ctgattgacc gcgttgtctt ccgccagcag gatccgcagt ggatggcgct
     1801 tgcccatctc ggggtcgatc ttggtctttg ctcgcccatc cccggcggcc gccggttctt
     1861 ccgcaccggc aaacaccgcg atcagagcgt cgaagagttg ggagggcttg atcggcttgg
     1921 tgagctgagc cgcaaagtcc accccacgca gatcggcttc gcgcctcccc agggaggtaa
     1981 agaggaccat gggtaattcg gccgaagccc ggagctgccg gatctctttc gccaaggcca
     2041 agccatccat ctcaggcatg tgcatgtcca ggatagccaa atcgaacgac tcaccgcggc
     2101 gaatccaatc caatgccaat cgcggcgaac cggtttcgag tggggtcatg ccccacgact
     2161 tagtttgcac caccaggatt ctccgattgg tctcgttgtc atccacgatg agaactcgct
     2221 tcccgactag cttcggctgc tcggccgcga gctcggggcg ggcgacctgg ggcagctcgg
     2281 cggcctgggt cacgaccgta aagtgaaagg tcgagccgac tcccggctcg ctttcggccc
     2341 acatcttgcc acccatcagc tcggtcagcc gccggctgat ggcgagaccc aggccggttc
     2401 caccatgctt cctcgcggtc gaagcgtcgg cctggctgaa ggactggaag agccggccga
     2461 ttgcttcgtt tgagattccg agtccggtgt cacggatcga gaagtgcaaa tcgtgggccc
     2521 aagcccttcg cttggtgacc acgttgagca ccacttctcc ccgctcggtg aacttgaccg
     2581 cattcccgag caggttgatc aggatctggc gaagccgagt gacatccccc agtacccccc
     2641 gcggcacttc ttcatccaac tggtaggcca gttccagccc tttctccgcc gctcgagggg
     2701 cgaccagatc cagcgcgctc tcgacgcact cccgcaagtc gaagggctgg ctttccaaat
     2761 ccatcttgcc ggcttcgatc ttgctgaagt ccaagatgtc gttgatgatc gtgagcagcg
     2821 aatcgccgct gttgcgaatg atctcggcga actcgcgctg ctcgtcgttg agcgtggtat
     2881 ccaagagcag cccgctcatc ccgatgactg cattcatcgg ggtgcgaatc tcgtggctca
     2941 tcgtggccag gaaggcgctc ttggcttcgt tggcagcttc ggccgactgg cgggccagca
     3001 ccagctcggt aatgtcatag tagatcacaa tcacgccctg ctgctcttga cccaccttga
     3061 ccggcaacgc gagcacctct acgtctacaa gcgatccgtc cttgcgagtg cgacgagcga
     3121 tcgcccgcaa caattccccc gaggcggagt gcttgttgta attccgggcc tcgtcgcgaa
     3181 tctcatccag gtttgcgacc agatcgtcca ggttgcgccc gatcgcttcc tcggcggtgt
     3241 atccaaa
//
LOCUS       DATDPQ010000112         4677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1618239, whole genome shotgun sequence.
ACCESSION   DATDPQ010000112 DATDPQ010000000
VERSION     DATDPQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618239"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VI520_02545"
     CDS             complement(<1..879)
                     /locus_tag="VI520_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36793.1"
                     /translation="MQNAEKPTSVIGRAIALLLGLAFVVSLPLSILALAWGRVLFSPE
                     QMTDILAAELVDSGALQRAAVEAFLARSGPQEESNPQDGSNPGGGSLDFLDRQELDRL
                     VKTLFPPEWAHDQMRANVEILYAWIDSDLAQPAFRLDIQPLKDRLQAGGAQELVTVIV
                     DSWPVCNAEQVQQLEREGFATGELPSVSCEPPEPLRSRLINTGIELLLERSRSLPSSI
                     PIGGEAPASEDQQGLNQLKRGLRFLRELSDVGWMLPTSLLGLIVALAVRSWPALLRWW
                     GAPLLGSGLSSFLTLII"
     gene            complement(890..1933)
                     /locus_tag="VI520_02550"
     CDS             complement(890..1933)
                     /locus_tag="VI520_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLF36794.1"
                     /translation="MVATVETRKPKLNLAGLTKSDYTGNPTTLCAGCGHNAISSQIIS
                     AAYELSIRPHEIIKLSGIGCSSKSPAYFLGKSHGFNSLHGRMPSLATGALVANRTLKA
                     IGVSGDGDTASIGMGQFIHFLRRNVPMVYIIENNGVYGLTKGQFSATADKNQELKYAG
                     LNPFMPVDIALEAIAANCGFVARSFAGDPKQVTELIKAALSFEGTAVLDIISPCVTFN
                     NRDESTKSYGWGREHRDPLHDVTFVPKYEEIQAEYEPGEVIEVRLHDGSILVLKKIER
                     EYDPTNRLAAIQLLEDARAKQILATGLIYYSAEQASLQDSEGLIERPLAELQEGELRP
                     AKESLETVMADFR"
     gene            complement(1935..3758)
                     /locus_tag="VI520_02555"
     CDS             complement(1935..3758)
                     /locus_tag="VI520_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HLF36795.1"
                     /translation="MSEVLSLALPTAVGEVVNDFSMVVATANGSGSQTSNLALIRALF
                     RMGIPVSGKNLFPSNIQGLPTWYTIRASNSGHTARRDSVEILVAMNAPTFAEDLAGLA
                     TEGACFYADDLRLPTQREDVVFYPMPIKELVKQANPPKDLKDYIANMGYVGIVAQLLG
                     IELEAVAGALDTHFGGRQKPIEMNMGMVNRAAEWAEHNLEKRDPYRVARDHQTEGKIL
                     IEGNTAGALGAIYGGVSFASWYPITPASSFADAVREYLPKLRSAGHNGKHPFAVIQAE
                     DEISAIGMAVGAGWAGARAMTSTSGPGISLMAEYAGLAFFAEVPVVIWDIQRMGPSTG
                     LPTRTSQGDVLFVRYLGHGDTKNVILLPGSVAECFEFGWKAFDLAERLQTPVFVLSDL
                     DLGMNLWMSDPFEYPDAPMDRGKVLSEADLERLGQFARYRDVDGDGITYRTLPGNKHP
                     LSAWFARGTGHNEQAAYSERPDDWEQNLTRLMHKAETARQYVPHPVQSGSGTEKIGLI
                     YYGSTDPAVQEALERMEQGGLRLDALRLRAVPFTVEVREFIRSHDVVYVLEMNHDGQM
                     HKLLQLEVPEFATKLRSLTHNNGISLTARWIVTELQTAEED"
     gene            complement(3755..4489)
                     /locus_tag="VI520_02560"
     CDS             complement(3755..4489)
                     /locus_tag="VI520_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HLF36796.1"
                     /translation="MIDQGVDRSYFAASEFMRYLITLEAEPGTRLPPISELAQELGIS
                     SGKLREQLEVARELGLVEVRPKTGIRVRGYSFYPTVRTSLRFALALDPGYFDQFSQLR
                     NQVEASYWYEAVALLSEADKEGLRQLMARAWAKLEGEPIQIPHLEHRELHLTIYSRLE
                     NAFVTGLLEAYWDAYEAIGLNLYEDYSYLRQVWSYHGQMVDAILTGDAEAGYRALVEH
                     TALLRTRPKPLLFPNGPVPRRGRRET"
BASE COUNT          852 a         1485 c         1413 g          927 t
ORIGIN      
        1 gatgatcagc gtgaggaagc tgctcaagcc ggaacccagc agaggcgcgc cccaccagcg
       61 caacagcgcc ggccaggagc ggaccgcgag cgcaacgatc aggccaagca aggaggtcgg
      121 cagcatccat ccaacgtccg acagttcccg aagaaaccgc agaccccgct tcaattgatt
      181 caacccttgt tgatcttcgc tggccggcgc ctccccgccg atcgggatgc tggagggcaa
      241 acttctggaa cgttcgagca acagctcgat gccggtattg atcaggcggc tacgcagggg
      301 ttcaggcggt tcgcagctga cagagggcag ctctccagtg gcgaaaccct cccgctcgag
      361 ctgctgaacc tgctccgcgt tgcagaccgg ccacgagtcc acaattaccg tcaccagctc
      421 ctgtgctcca cctgcctgca ggcggtcttt cagcggctgg atgtcaagcc ggaaggccgg
      481 ttgggccaga tcgctgtcga tccaggcgta caggatttca acgttggctc gcatctgatc
      541 gtgagcccac tcgggcggga agagcgtctt aaccagccgg tccagctcct gccggtcgag
      601 gaaatccagc gatccgcccc ccgggttgga tccgtcttgt gggttcgatt cttcttgcgg
      661 cccactccgg gctaagaagg cctcgaccgc cgcccgctgc agggcaccac tgtctaccag
      721 ctccgcagcc aggatgtccg tcatctgctc cggcgagaac agcaccctgc cccaggctag
      781 ggccaggatg gaaagcggca gactgaccac aaaggccagc ccgagcagca gggcaatcgc
      841 ccgcccaatc acgctggttg gtttctctgc gttttgcaaa gcggttcgct cagcggaagt
      901 ccgccatcac ggtctcgagg ctttccttcg ccggccgcag ttcgccttcc tgcagctcgg
      961 cgagcggccg ctcgatcagc ccttcggaat cctgcaggct ggcctgctcg gcgctgtagt
     1021 agatcaggcc ggtggcgagg atctgcttgg cccgggcgtc ctccagcagc tgaatcgcgg
     1081 ccagccgatt ggtcggatcg tactcccgct cgattttttt caggaccagg attgatccgt
     1141 cgtgcagccg gacctcgatc acctctcccg gctcatattc cgcctggatc tcctcgtact
     1201 tcggcacgaa ggtcacatcg tgcagtgggt ctcggtgttc gcgcccccag ccgtaactct
     1261 tggtcgattc gtcccggttg ttgaaagtta cgcaggggct gatgatgtcg agcaccgcgg
     1321 tgccttcaaa gctcagggcg gccttgatca gctcagtcac ctgctttgga tcgccggcga
     1381 acgagcgagc gacgaagccg cagttggcgg caatcgcctc caaggcaata tccaccggca
     1441 tgaacggatt cagtccggcg tatttcaact cttgattctt gtcagcagtc gccgaaaact
     1501 ggcccttggt caacccgtat accccgttgt tctcaatgat gtacaccata ggcacgttgc
     1561 ggcgcaggaa gtggatgaac tggcccatgc caatgctggc cgtgtcgcca tccccgctga
     1621 ccccaatcgc cttcagggtc cgattggcga ccagggcgcc ggtcgccagc gagggcatcc
     1681 gaccatgcag gctgttgaag ccgtgcgact tgcccagaaa ataggccggg ctcttgctgg
     1741 agcacccgat cccggagagc ttgatgatct cgtgcggccg gatcgagagc tcataggcgg
     1801 cactgatgat ctggcttgag atcgcgttgt ggccgcatcc ggcgcacagg gtggtcggat
     1861 tcccggtgta gtcggacttt gtgagtccgg ccaggttgag ctttggcttg cgggtctcga
     1921 ctgtcgcaac catcttagtc ctcctccgcg gtttgcagct cggtgacgat ccaacgggcc
     1981 gtcagcgaga tcccgttatt gtgagtcagg gatcgcagct tggttgcaaa ctccggaact
     2041 tcaagctgca gcagcttgtg catctggccg tcgtggttca tttccaacac gtacacgacg
     2101 tcgtggctgc gaatgaattc tctgacctcg acggtgaagg gaaccgcccg cagcctcagg
     2161 gcgtctaggc gcaggccacc ctgctccatc cgctcgagcg cttcctggac cgccgggtcg
     2221 gtcgatccgt agtagatcaa tccgatcttc tcggttcccg accccgattg caccgggtgt
     2281 ggaacgtact ggcgagcggt ttcggcctta tgcatcagtc gagtgagatt ctgttcccag
     2341 tcgtccggcc gctcactgta ggccgcctgt tcattgtggc cggtgccacg ggcgaaccag
     2401 gccgagagcg gatgcttgtt tcccgggagc gtgcgatagg tgatgccgtc tccgtccacg
     2461 tcccggtagc gggcgaattg tcccaggcgc tccaggtccg cttcgctcag cacctttccc
     2521 cggtccatcg gcgcgtcggg atattcaaat gggtccgaca tccacagatt catccccagg
     2581 tcgagatcgc ttagcacgaa gaccggggtc tgcaatcgct ccgcaagatc gaacgccttc
     2641 cagccaaact cgaaacactc ggcgaccgag cctgggagca ggatcacgtt cttcgtatcc
     2701 ccgtggccaa ggtagcggac gaagagcacg tcgccttgcg aggtccgggt cgggaggccg
     2761 gtgctgggac ccatccgctg gatatcccag atcactaccg ggacctctgc gaagaacgcc
     2821 aatccggcgt attcggccat cagcgagatt ccgggacccg aagtggaggt catcgcccga
     2881 gccccggccc accccgcccc gaccgccatt ccgatcgcgg agatttcgtc ctccgcctgg
     2941 atgacggcga atggatgctt cccgttgtgg ccggcggatc gcagcttggg cagatactcg
     3001 cggaccgcgt cggcgaagct tgaagccggg gtaatcggat accaggaggc gaagctcact
     3061 ccaccataga tcgcgccgag cgcgccggct gtgttgccct cgatgaggat cttcccctcg
     3121 gtctggtggt cgcgggcgac ccgataggga tcccgctttt ctaggttgtg ctctgcccac
     3181 tcagccgctc gattgaccat gcccatgttc atttcaatcg gcttctgccg gccgccgaag
     3241 tgagtgtcca gcgccccggc caccgcctcc aactcgatcc ctaagagctg agcgacgatc
     3301 ccgacgtatc ccatgttggc aatgtagtct ttcaggtcct tgggcgggtt ggcctgcttg
     3361 acaagctctt tgatcggcat cgggtagaac acgacgtctt cccgctgcgt cggaagtcgc
     3421 agatcgtcgg catagaagca ggcgccctcg gttgccaggc cggcaagatc ttcggcaaag
     3481 gtaggcgcgt tcatcgcgac caggatctcg accgagtcgc ggcgggcggt atggcccgaa
     3541 ttgctggctc ggatcgtata ccaggtgggc agtccctgaa tgttcgatgg gaacaggttc
     3601 ttcccactga ctggaattcc cattcgaaac agagcccgga tgagggccaa attggaagtc
     3661 tgacttcccg agccgttcgc ggtcgccaca accatcgaga aatcgttgac cacctctccc
     3721 acggcagtgg gcaatgccag gctgaggacc tcgctcatgt ctccctcctt ccccgccgcg
     3781 ggaccggccc gttcgggaag agcagcggtt tcggccgggt ccgcaacaag gcggtatgtt
     3841 cgacgagagc tcgatagccg gcttccgcat cgccggtcag gatcgcgtcg accatttggc
     3901 cgtgataaga ccaaacctgc ctcaagtacg aatagtcttc gtacaagttg agcccgatcg
     3961 cctcgtaggc gtcccagtag gcctccagga gaccggtcac aaaggcattc tcgagccgcg
     4021 agtagatcgt aaggtgcagc tccctgtgtt ccaggtgcgg gatctgaatc ggttcacctt
     4081 ccagcttggc ccaggcccgc gccatgagct ggcgcagccc ctctttgtcc gcctcgctaa
     4141 gcagcgcaac cgcctcgtac cagtacgagg cttcgacctg attccgcagc tggctgaact
     4201 ggtcgaaata gcccgggtcc agcgcgagtg caaaccgcag gctggtacgc acggtcgggt
     4261 aaaacgagta tccacggact cggatcccgg tcttgggccg gacctccaca agtcccaact
     4321 cgcgagcgac ctccaattgc tcccgcagct tcccggagct gattccgagt tcctgagcga
     4381 gttcgctgat cggcgggagc cgggtgcccg gctcagcctc cagcgtgatc aggtagcgca
     4441 tgaactctga ggctgcgaag taagatcggt cgacgccttg gtcaatcata ggattcatta
     4501 tatcaataag aggattgctc ggtgtatcca cctttcgggg gatcggcttg gccgctctgg
     4561 tacaatgcgg cggtgctccg ccagggctac ggtggaccaa actgaagcgt taagcctgat
     4621 gcaggagtac gtcaagaatc caggcctcca gcggcacatg ctggcggtgg cggcggc
//
LOCUS       DATDPQ010000113         4117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3706178, whole genome shotgun sequence.
ACCESSION   DATDPQ010000113 DATDPQ010000000
VERSION     DATDPQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3706178"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..2807)
                     /locus_tag="VI520_02565"
     CDS             complement(243..2807)
                     /locus_tag="VI520_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLF36797.1"
                     /translation="MEAITLQLTLAARYMNGRRLRTVLTTLAVVFGVLLTFGVNTILP
                     AISQSFRANMLAIAGQVDLTITHKASAAFPTQVSEKLRAIEGIFAVSGSLNRGVNLPV
                     DYFDRDPGQRDRVTALSLVGIDPDAAQTLRAYPVVAGRFFQEGDSNVAIISRSLAEAL
                     DLRVGERLTLPTTQGQAELSVVGILPARTLPGNEEVLVTLSEAQRLFNQPDQINAIDL
                     NFETLDEQLRAEIQNAVEAAVGSDFHLGGLSSGSELLTNLQIGQAMINVMGALGLFMG
                     GFIIFNTFRTIVTERRHDLGMLRSLGADRRMITGLILTESVLQGLVGTAAGLVLGYGF
                     GAGLLGLLGKTFEQYMHVRVGPPVVSPALLVASVAVGVGLTLVAGLLPALKAGRVSPM
                     EALRPPVAELRSARAEGAAFVIGAVMIGLALASLLSRNSGLLALGGVLFLIGLVLAGP
                     VLVRPIANAFGALLSRLTHQEGIVQLAEGNLTRQPTRAAVTASATMIGLAVIVMAGGL
                     LSSVSVGFLDALERSLGSDYLFIPPGIAVWGGNVGAAPALADELRAVPSIEAVSSLRV
                     ATSLAGELPLTVLAIDPVNYPKVSGLVFQQGDPDSAYSSLAAGRSVIANGPLAALAGL
                     SPGDEISLLTAVGQQSYRVVAVANDYLNAKIATGYISQASLATDFHIIEDVMLQANLK
                     PGADRVEAEAQIKELATQYPQFRLIAGQEYYDENKRIFDAAFLGLYVVLGFLAIPSLV
                     AMLNTLAIGVIERTREIGMVRAVGATRQQIRRMVVLEALILASLGTAFGLLSGIYLAR
                     LAVDSLGAAGYPVRASFPWMGAVLAVAIGLLFGALAAVIPARQAAKLDIVAALRYE"
     gene            complement(2826..3596)
                     /locus_tag="VI520_02570"
     CDS             complement(2826..3596)
                     /locus_tag="VI520_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36798.1"
                     /translation="MSDLTANQNPAAVSTENLTKIYGKAEAAVTALDHIDMRIEVGEF
                     VAVMGPSGCGKSTLLHLVGGLDQPTEGRVFLNGADLSALDDEAISILRRRNIGFVFQF
                     YNLLPVLSALENAALPLLLDGVESGRARKTAAEWLQRFELEDRLQHRPDQLSGGQQQR
                     VAIARALVAEPSLVLADEPTGNLDSQAGDQIAALLGRVSSEWGRTVLIATHDPRIAAQ
                     ADRILFLKDGKLVDETVLEPTDGRNTEAVADRIRRMGG"
     gene            complement(3589..>4117)
                     /gene="mce"
                     /locus_tag="VI520_02575"
     CDS             complement(3589..>4117)
                     /gene="mce"
                     /locus_tag="VI520_02575"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HLF36799.1"
                     /translation="EFWRDVLGLELAHVEDAPDQGSIVAFLSTGDQEVELVKPTTDNS
                     GIARYLERRGPGLHHICLEVDDIQECLAQLKARGVRLIDPEPIVGTGGKRIAFIHPES
                     THGVLVELYELTHEESEIRLARARGLADRALAQGQSVAASVLEFLRHLRLDGGRRRRD
                     RAREQPTAETGEDDV"
BASE COUNT          767 a         1341 c         1321 g          688 t
ORIGIN      
        1 ggaggcggcc gaactgctga tcggtcagcg gatcacagcg gaacggatga atgaagcggc
       61 ggagatggct gcccgccggg ccaagccgct cgacaacacg gacatggggc tgagcttccg
      121 caagaagatg gcgcgggtct acgtagcccg cgcgctagcg gaggctgctg gatggtgatc
      181 tccgcgactg aggggaggcc cgagcgctcg cgcgggctgc ctgagatccg acccgtgcct
      241 ggctactcgt aacgcagcgc ggccacgatg tccagcttcg cggcctgccg ggccgggatc
      301 accgccgcca gcgcgccgaa caacagtccg atcgctaccg ccaacacggc tcccatccac
      361 gggaaggagg cccggaccgg gtagcctgcg gctccgagcg agtccaccgc caggcgagcc
      421 aggtatatcc cgctaagcag cccgaaggcc gttcccaggg acgccagaat cagcgcctcc
      481 agcaccacca ttcgccggat ctgctggcgg gtggccccca ccgcgcggac catcccgatc
      541 tcccgggtcc gctcgatgac cccgatcgcc agtgtgttca gcatcgcgac cagcgacggg
      601 atcgccagga accccagcac cacatatagg ccaaggaaag ccgcgtcgaa gatccgcttg
      661 ttttcgtcat agtactcctg gccggcgatc aaccggaact gcggatattg agttgcgagc
      721 tccttgatct gggcctccgc ctccacccga tcggcgcctg gcttgagatt ggcctgaagc
      781 atcacatcct cgatgatgtg gaagtcggtt gccagcgatg cctgggagat gtaacctgta
      841 gcgatcttgg cgttgaggta gtcgttggcc actgccacga cccggtacga ttgttggcct
      901 acggctgtga gcaggctgat ctcatcgcct gggctgagac ccgcgagggc ggccagcgga
      961 ccgttggcga tcacgctgcg ccccgcggcc agcgaactgt aagccgaatc cgggtctccc
     1021 tgctgaaaca ccaggccgct gaccttcgga tagttcacgg gatcgattgc caggaccgtg
     1081 agcggaagct cgccggccag cgaggttgcg actcggaggc tggagaccgc ttcgatactc
     1141 ggaaccgccc gcagctcgtc cgccagggcc ggggccgcac cgacgttacc gccccaaacg
     1201 gctatccccg ggggaatgaa caggtagtcg ctccccaggc tgcgctccag agcgtcgagg
     1261 aacccgactg agacgctgct gagcagacca cccgccatca cgatcactgc caggccgatc
     1321 atggtagcgc tggccgtcac cgcggctcgg gtgggctgcc gggtcaagtt accctcggcc
     1381 agctggacaa tcccctcttg gtgggtcagc ctggagagca aagcgccaaa ggcatttgcg
     1441 atcggccgca ccagcaccgg cccggccagc accagcccga tcaggaagag gactccgccc
     1501 aatgcaagga gacccgaatt tcgggatagc agactcgcca gcgcaagccc gatcatgact
     1561 gccccgatga cgaacgctgc cccctccgcc cgagccgagc ggagttcggc gaccggcggc
     1621 cgcaaggcct ccatcggcga gacccggccg gctttcagag caggcagcag gccggcaacc
     1681 aaggtcaggc ccaccccgac ggcaaccgac gcgaccaaca aggctgggct gacaactggg
     1741 ggcccaaccc gcacgtgcat atactgctca aatgtcttcc cgaggagtcc cagcaggccc
     1801 gctccgaacc catagcccag tacaaggccg gctgccgtgc cgaccaaccc ctggagcaca
     1861 ctctcggtga gaatgagccc cgtaatcatt ctccgatcag cgcccagtga gcgcagcatc
     1921 cccagatcgt ggcggcgctc ggtcacgatg gtgcggaagg tgttgaagat aatgaagccc
     1981 cccatgaaga ggccaagggc acccatgacg ttgatcattg cctggccgat ctggagattg
     2041 gtgagcagct cgctcccaga agacagaccg ccgaggtgga aatccgagcc cacggccgcc
     2101 tcgaccgcat tttgaatctc cgcccgcagc tgctcatcca atgtttcgaa gttcagatcg
     2161 atggcgttga tctggtcggg ttgattgaac aggcgctgag cttcgctcag agtgactaaa
     2221 acctcttcgt ttcctggcag ggtccgcgcc ggcaggattc caacgactga cagttctgcc
     2281 tgcccctggg tggtcgggag ggtgaggcgt tcgccgaccc ggaggtccaa tgcctcggcc
     2341 agactgcggc tgatgatggc cacattggag tcgccctcct ggaagaaacg tccggcgacc
     2401 actgggtagg cgcgcagagt ctgggccgca tccggatcga tccccaccag ggatagagcg
     2461 gtcacccgat cgcgctgacc cggatcgcga tcgaagtaat cgaccgggag attgaccccc
     2521 cggttgagag agcctgagac ggcgaagatg ccttcaatcg cccgcagctt ctccgacacc
     2581 tgggttggaa atgcggcgct tgccttgtgg gtaatagtca agtcgacctg gccggcgatc
     2641 gccagcatgt tggcccggaa ggactgggag atggcgggca ggatcgtgtt gactccgaaa
     2701 gtgagcagca ccccgaaaac caccgccagg gttgtgagga ccgtgcgcag cctgcgacca
     2761 ttcatgtagc gggcagccaa cgtcaattga agggttatgg cttccatcct gaaccctgag
     2821 ccggctcagc ctcccatgcg cctaatccgg tcggcgacgg cctcggtgtt gcggccgtcc
     2881 gtaggttcca ggacggtctc gtcgacgagc ttgccgtctt tgaggaacag gatccggtcg
     2941 gcctgggcgg cgatccgcgg gtcgtgagtg gcgatgagca ccgtccggcc ccactcgctg
     3001 gagaccctgc ccaacagggc ggcgatctga tcgccggcct gagagtcgag attgccagtg
     3061 ggttcatcgg caaggaccaa tgacggctcg gcgaccaggg cgcgggcgat ggcaacccgc
     3121 tgctgctggc cgcctgagag ttggtctgga cggtgctgca ggcggtcctc tagctcgaag
     3181 cgttggagcc attctgcggc cgtcttgcgg gcgcgcccgg attcgactcc gtcgagcagg
     3241 agcggcaggg cggcgttctc gagcgccgac agcaccggca gcaggttata gaactgaaag
     3301 acgaacccga tgttgcgccg gcgcaggatc gagatggctt cgtcgtccaa cgcggagagg
     3361 tcggcaccgt tgaggaacac gcgcccttcg gtcggctgat cgagtccgcc gaccaggtgc
     3421 agcagggtgg attttccaca accgctgggg cccatgaccg cgacaaactc gccgacctcg
     3481 atgcgcatat cgatgtgatc gagcgcggtg acggcggcct ccgccttgcc atagattttg
     3541 gtgaggtttt cggtcgatac tgccgccggg ttctggtttg cggtgaggtc agacatcgtc
     3601 ttcaccggtc tcggcggttg gctgctccct cgcccggtcg cgcctgcgtc ggccgccatc
     3661 caaccgcaag tgtcgaagga actcgagcac actggccgcc acgctttgac cctgggccag
     3721 ggcccgatcg gcgagcccgc gggcgcgggc caggcggatc tcagactctt cgtgagtcag
     3781 ttcatataac tcgaccagca ccccgtgggt gctttccggg tggatgaagg cgatccgttt
     3841 tccgccggtt ccaacaatgg gttccggatc gatcagccgc accccgcgcg ctttcaattg
     3901 ggcgaggcat tcctggatat cgtcaacctc gaggcaaata tgatgcaggc cgggcccccg
     3961 gcgctctaag tagcgggcga tcccactgtt atcggtggtc ggcttgacca gctccacttc
     4021 ctggtctccg gtggacaaga acgccacgat cgaaccctga tccggagcgt cttcgacatg
     4081 ggccagttcc agtcccagca cgtcccgcca gaactcc
//
LOCUS       DATDPQ010000114         3112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2394463, whole genome shotgun sequence.
ACCESSION   DATDPQ010000114 DATDPQ010000000
VERSION     DATDPQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394463"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VI520_02580"
     CDS             <1..1190
                     /locus_tag="VI520_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF36800.1"
                     /translation="AESENTGKPYSFVSLGADLPFTVDNLRFFASAARDMHGSHAGEY
                     LAGYTSIFRREPVGVVGQITPWNYPLMMAIWKIGPALAAGCTAVLKPAPTTPLTTLML
                     AELSKEAGLPDGVFNIVTGGNESGQALVEHPDVRMVSLTGSPRTGMRVMETASKTLKR
                     VHLELGGKAPLLVFDDADADLVVSKVTLAATFNTGQDCTAATRVYVDQGRYKSLQESL
                     VESMRAVKLGEPFADGVAMGPLISAVQRERVQGFIERAKAGGAQVLTGGGIPKGISKG
                     FYFEPTVVIDAAQHSEIIQSEVFGPVLTISPFKDAEEALTLANDVPYGLSASIFTKDI
                     GRAMKLSANIEAGTVWVNDHIPLTSETPHGGFKHSGFGKDLSAEAMGDYQITKHVMVS
                     HTG"
     gene            1187..2230
                     /locus_tag="VI520_02585"
     CDS             1187..2230
                     /locus_tag="VI520_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36801.1"
                     /translation="MSSGSPPAIRLDRVSRQFGDVKAVDDVSLDIPDGEFFTLLGPSG
                     SGKTTSLRLIAGFDHPSAGRILLHGEDVTNLPPYEREVNTVFQDYALFPHMSVGENIG
                     YGLMIRSLPKPERAKQVAEMMELVRMPGLEDRRPSQLSGGQRQRVALARALILRPRVL
                     LLDEPLGALDLKLRQQMQVELKSIQEQVGITFIYVTHDQEEALTMSDRIAVFNLGRVV
                     QIGSPAELYEHPASPFVAGFVGVSNLISGELARQITTSPETFSVRPEKISLQALDQPA
                     PNGYSSVEGQVRDVVYLGIHTRYLIELDSGEDLTVVEQNRDTTSMDVMSARGRRVRLV
                     WDPAHNQRVDVNA"
     gene            2279..>3112
                     /locus_tag="VI520_02590"
     CDS             2279..>3112
                     /locus_tag="VI520_02590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36802.1"
                     /translation="MTAIALGPAAPRPLLHRLGTFIYLRPWLVLALLLGPPLLWMVVI
                     YLGSIAALILQSFFSLDPFTGQVIREFTLSTYRELFVPAHLEIFLRTALMAAAVTIAT
                     AVLAFPFAYFMARFASRRTKAALYLAVLLPLWSSYLVRVYAWRLILAKEGILNWFLER
                     LNLLGVLEAALRIPVIGGPSLSVSFLGMFIVFVYVWLPYMILPIQAALERVPQSLLEA
                     SGDLGARPRRTLRHVTLPLAFPGVLAGSIFTFSLTLGDFVVPYSLGNSSFFIGQAVLS
                     FT"
BASE COUNT          516 a          991 c          988 g          617 t
ORIGIN      
        1 gagccgagtc ggagaacacc ggaaagccct acagcttcgt cagcctgggc gccgacctgc
       61 ccttcacggt cgacaacctg cgcttcttcg cctcggccgc ccgggatatg catggttcgc
      121 atgccggcga gtacctcgcc ggttacacct cgattttccg ccgcgaaccg gtcggggtgg
      181 tcggccagat cactccctgg aactatccgc tcatgatggc gatttggaag atcggcccgg
      241 cgctcgcagc gggctgcacc gccgtgctaa aaccggcgcc gacgactccg ctcaccacgt
      301 tgatgctcgc cgagctctcc aaagaggcgg ggctgccgga tggggtcttc aatatcgtca
      361 cgggtggcaa tgagagcggt caggcgctgg ttgagcaccc cgacgtgcgg atggtgagcc
      421 tcaccggctc gccgcggacc gggatgcggg tgatggagac ggcttcaaag accctcaaac
      481 gagtgcacct cgagctggga ggcaaggctc cgctgctggt cttcgacgac gcggatgccg
      541 acctggtggt ttccaaggtg accctggcgg cgactttcaa caccgggcaa gactgcacgg
      601 cggccacccg ggtgtacgtg gaccagggtc gctacaagtc tctgcaggag tcgctggtgg
      661 aaagcatgcg ggcggtgaag ctgggtgaac cgttcgccga tggcgtggcg atgggccccc
      721 tgatctcggc agtccagcgc gagcgggtgc agggcttcat cgagcgggcc aaggccggcg
      781 gcgcccaggt gctgaccgga ggggggatcc ccaaggggat ttcgaagggc ttctatttcg
      841 agccgaccgt cgtgatcgac gccgcccaac attcggagat catccagagc gaagtgttcg
      901 ggccggtgct gaccatcagc ccgttcaagg acgctgagga agcgctgact ctcgcaaacg
      961 acgtgcctta tgggttgtcg gcctcgattt tcaccaaaga tatcggccgg gccatgaagc
     1021 tctcggccaa catcgaggcg ggcacggtct gggtcaacga tcacattcca ctcacctctg
     1081 aaacccccca tggggggttc aaacactccg gtttcggcaa ggacctgtcg gccgaggcga
     1141 tgggggacta ccagatcacc aaacacgtca tggtcagcca caccggatga gctccggctc
     1201 tccgccggcg atccggctcg accgggtgag ccgccaattc ggagacgtca aagccgtcga
     1261 cgatgtcagc ctggacatcc cggacggcga gtttttcacg ctgttaggcc cctctggatc
     1321 aggcaaaact acctccttgc ggctgatcgc cgggttcgac catccgagcg ccggccggat
     1381 tctgctgcac ggggaggacg tgaccaacct tccaccctat gagcgggagg tcaacacagt
     1441 cttccaggac tatgccctct ttccccacat gtcggtgggc gagaacatcg gctacgggtt
     1501 gatgatccgc agcctcccca agccggagcg tgccaagcag gttgcagaaa tgatggagct
     1561 ggtccgaatg cccggcctgg aagatcgccg accttcgcaa ttgtctggcg gtcagcgcca
     1621 gcgggtggcg ctggctcgag ctctcatctt gcggccgcgg gtgctgctgc tggacgagcc
     1681 gctgggggcg ctggacctga aactgcgcca gcagatgcag gtcgagctga agtccatcca
     1741 ggagcaggtg gggatcacct tcatctacgt cacccacgat caagaagagg cgctcacgat
     1801 gagcgatcgg atcgcggtgt tcaatctcgg tcgggtcgtc cagatcgggt ctccggccga
     1861 gctctacgag cacccggcct ctccatttgt agcgggtttc gtcggagtgt ccaacttgat
     1921 cagcggtgag ctggcgaggc agatcaccac ctcgccggaa accttctcgg tccggccgga
     1981 aaagatcagc ctccaagcac tcgatcagcc cgccccgaac gggtacagct cggtcgaggg
     2041 ccaggtgcgc gacgtggtgt atctcgggat ccacacccgg tacctgatcg agctggacag
     2101 cggagaagat ctgacggtcg tcgagcagaa ccgcgacacc acttcgatgg atgtgatgag
     2161 cgcccggggc cggcgggtgc gcctggtttg ggaccccgcc cacaatcagc gagttgatgt
     2221 gaatgcttga gccgcagacc cggggccgcg ggccattcgg ctccgaggaa acgaccgcgt
     2281 gacggccatc gcgctgggtc cggccgcccc ccgcccgctc ctgcaccggc tgggtacctt
     2341 catctatctc cggccttggc tggtattggc gctgctcctt ggaccaccgc tgctgtggat
     2401 ggtcgtgatt tatctcggat ccatcgctgc gctcattctg cagagcttct tctctctcga
     2461 tccatttacc ggccaggtga tccgggagtt caccctgtcg acctaccggg agttgttcgt
     2521 tccggcccac ctcgaaatct tcttgcggac cgcgctcatg gcggccgcgg tgacgatcgc
     2581 cactgcggtc ctggcgtttc ccttcgccta cttcatggcc cgcttcgcct cgcgccggac
     2641 aaaggccgcg ctctacctgg ccgtgctgtt gccgctgtgg tcttcctacc tggtgagggt
     2701 ctatgcctgg cggctgatcc tggccaagga ggggatcctg aattggttcc tcgagaggct
     2761 gaatctgctg ggcgttttgg aggcagccct gcggattccg gtgatcggcg gtccgtcgct
     2821 ctcggtctcc ttcctgggaa tgttcatcgt gttcgtgtat gtctggttgc cctatatgat
     2881 cctgcctatc caggcagccc tcgaaagggt tccgcagtcg ctcctggagg cctccggcga
     2941 tctgggggct cgacctcgcc gcacgctgcg ccacgtcact ttgccgctcg ccttcccggg
     3001 ggtgctggcc ggatcgatct tcactttctc gcttaccctc ggcgatttcg tggtgcccta
     3061 ttcgctcggc aactccagtt tcttcattgg ccaggcggtg ctttcgttca cc
//
LOCUS       DATDPQ010000115         3077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1357904, whole genome shotgun sequence.
ACCESSION   DATDPQ010000115 DATDPQ010000000
VERSION     DATDPQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357904"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="VI520_02595"
     CDS             complement(<1..1906)
                     /locus_tag="VI520_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HLF36803.1"
                     /translation="MKSRTPLPIAALLAVLAGLVASPVYADGIVVPEPPICEFGPCPV
                     PIPMAQLAIEYHRVQVRIEDQVAVTHVDQAFRNDNDWTVEGTYIFPLPAGAAVSNFTL
                     WIDGEPVEGKVLDREQARQIYQEIVRKLQDPALLEYVDRGAVQASIFPIEPGESRRIE
                     LEYTQVLEADHGLIGYRYPLNTEKFSAQPLEQVSIAVQVLSADPVRAVYSPSHEVDID
                     RSDDFEFRVGYEAADVTPDRDFELFYSVADDEIGLNLLSYRDPESEDPDGTFLLLVAP
                     SVQADPDRRVDKDLILVLDQSGSMEGEKFRQAQQALTFVLDHLNSGDRFNVIAFSTGT
                     DSYAGGLRPAEEATEARRWVERLTAVGSTDINRALLEAVAQLDRERSTFVIFLTDGLP
                     TEGVTEAEQILENLQEAAPSNLRLFTFGVGFDVDTFLLDSLANEHHGAASYVSPGQSI
                     DEALSAFYEKVSTPVLTNLELEFGNQIVFDLYPDPLPDLFAGGQLVLVGRYRDSGNFT
                     IRLSGEVNGETQTFSYEGQRLLSSGGPEFLPRLWATRKIGALLSQIRLEGPNQELVDQ
                     IVRLSIRYGIVTPYTSYLVTEPEALGADAQEDLAARAFSDLLGTPTAVSGEAAVQRAA
                     EEGQILRSDVA"
     gene            2076..>3077
                     /gene="prfA"
                     /locus_tag="VI520_02600"
     CDS             2076..>3077
                     /gene="prfA"
                     /locus_tag="VI520_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLF36804.1"
                     /translation="MLKKLTNLEERFQALEQQMAEAASDYQRLAELARERAELEPMVT
                     AFREYRDLSDRLSQAQSFSEGEDPELQELAQQEIQEIQPRLDGLESRLKDMLMPRDPR
                     DRRDVIMEIRAGTGGDEAGLFAADLFRMYLRYAERLGWKAEVLSQSDTGVGGLKEVVF
                     QIKGSGAFSKLKFESGVHRVQRVPATESQGRIHTSTATVAVLAEAEEVEIELPDKDLE
                     IETYRSGGAGGQNVQKNETAVRINHRPTGIVAQCQDERSQLQNKQRALRILRARLYEL
                     KIREQQAAQDATRRAQVGSAERSEKIRTYNFPQSRITDHRIGYTTHNLAGVLNGDLED
                     "
BASE COUNT          621 a          941 c          990 g          525 t
ORIGIN      
        1 acgcgacgtc ggaccgcagg atctgaccct cctccgcggc tcgctgcacg gcagcttcgc
       61 cggagacggc ggtcggagtg cccagcagat cggagaacgc ccgggcggcc aggtcttctt
      121 gagcatcggc gccgagggcc tcgggttccg tcaccaggta ggaagtgtac ggagtgacga
      181 tcccatagcg gatactgagc cgcacgatct gatccaccag ctcctgattg ggtccttcca
      241 gccggatctg gctgagcagc gcgccgatct tgcgggtggc ccacaggcgg ggcaggaatt
      301 ccggcccgcc tgaggatagc agccgctgac cctcgtaact gaaagtttgg gtctctccat
      361 tcacttcgcc gctcagccgg atcgtgaagt taccggagtc tcgatagcgg cccaccagca
      421 ccagctgccc gccggcgaag agatcgggaa gcggatcggg atagagatcg aagacgatct
      481 gattaccgaa ctcaagttcc aggttggtca agaccggcgt actcaccttc tcgtaaaagg
      541 ccgagagggc ttcgtcaatc gactgccccg ggcttacgta gctggctgcc ccatggtgtt
      601 cattggccag cgaatcgagc aggaaggtgt cgacgtcgaa acccacaccg aaagtgaaca
      661 gacgcaggtt ggagggagcc gcttcctgaa ggttctcgag aatctgttcg gcctcagtca
      721 caccctcggt cgggagcccg tcggtcagga agatcacgaa agtcgagcgt tcgcggtcga
      781 gctgcgccac ggcttccagc agggcccgat tgatgtcggt cgagcccacc gcggtgagcc
      841 tctccaccca acgcctggcc tcggtggcct cctcggccgg ccggaggcca ccggcgtagg
      901 agtcggtgcc ggtgctgaag gcgatgacgt tgaaccggtc gccagaattc aggtggtcga
      961 gcacgaaggt cagcgcctgc tgagcctggc gaaacttttc gccctccatg cttccgctct
     1021 ggtcgagcac caggatcagg tctttatcaa cccgccgatc tggatcggcc tggacgctgg
     1081 gggcaaccag cagcaagaat gtaccgtccg gatcctctga ctcgggatcg cggtagctga
     1141 gcaggttgag tccgatctcg tcgtcggcga cgctgtagaa aagctcgaaa tcgcgatcgg
     1201 gcgtgacgtc cgccgcttca tatccaaccc ggaattcgaa gtcgtccgag cgatcgatgt
     1261 ctacctcatg agaaggggaa tagacggccc ggaccggatc ggctgagagc acctgcaccg
     1321 cgatgctgac ctgctcgagc ggttgggccg agaacttctc ggtgttgagc ggatagcggt
     1381 aaccaatcag tccgtgatcg gcctccagga cctgggtgta ttccaactcg atccggcggc
     1441 tctctcccgg ttcgattgga aagatcgaag cctggaccgc acctcggtcg acgtactcca
     1501 gcagcgccgg atcttgcagc ttgcgaacga tctcctggta gatctggcgg gcctgctccc
     1561 ggtccaagac tttgccctcg accggctcgc cgtcgatcca gagcgtgaaa ttggagaccg
     1621 ccgcgcccgc cggtaagggg aagatgtacg tcccctccac ggtccagtca ttgtcattcc
     1681 ggaacgcctg atcgacatgg gtcacagcga cctgatcctc gatccggacc tggacccgat
     1741 ggtattcgat ggccagctga gccatcggaa tggggaccgg acatggcccg aattcacaaa
     1801 tcggcggctc tgggacgacg atcccgtcgg catagacggg gctcgccaca agaccggcga
     1861 gcacggccaa caaagcagca atggggagcg gggtgcgcga tttcatggga cgtctcctcg
     1921 ggtaagggaa cttgctctaa agacgtccgc cgctccggtc gggttcccct tccgttgaag
     1981 ttgcagtcaa tgcgctgggc cgagctccaa aggcttcggg tataattccg cccagacctc
     2041 ccaccgcacc tgtgggagtt tcttgtgtat aaggcatgct aaagaaactg acgaacctgg
     2101 aagagcggtt ccaggccctg gagcagcaaa tggcggaggc cgccagcgac taccagcggc
     2161 tggccgagct ggcccgcgag cgggccgagc ttgagccgat ggtgacggcc ttccgcgaat
     2221 accgcgacct gagcgaccgg ttgagccagg cccagagctt cagtgaaggc gaggatcccg
     2281 agctgcagga actggcgcag caggagatcc aggagatcca gcctaggctg gatggcttgg
     2341 aaagtaggct gaaagacatg ctgatgccgc gcgacccccg cgaccggcgg gacgtcatca
     2401 tggaaattcg ggccgggacc ggcggcgatg aggctgggct gttcgccgcc gatctgttcc
     2461 gtatgtacct ccgctacgcg gaacggctgg ggtggaaagc cgaggtgcta tcgcagagcg
     2521 acaccggggt cggcgggctc aaagaagtcg tcttccaaat caagggatcc ggagccttct
     2581 ctaagctcaa gttcgaatcg ggggttcacc gggtccagcg agtaccggcg accgaatccc
     2641 agggtcgaat tcacacctcg accgccacgg tggcggtgct cgccgaggcg gaggaagttg
     2701 aaatcgaact tcctgataag gatctggaga tcgagaccta tcgctcgggc ggggcgggtg
     2761 gacagaacgt gcagaagaac gagaccgcgg tccgcatcaa ccatcgcccg accggaatcg
     2821 tcgcccaatg ccaggacgag cgctcccagc tgcagaacaa gcagcgggcg ctgcggatct
     2881 tacgggcccg gctctacgag ctgaagatcc gtgagcagca ggccgcccag gacgccaccc
     2941 ggcgggctca ggttggcagc gcggaacgct ccgagaagat ccgcacgtac aacttccccc
     3001 aatcgcgcat caccgaccac cggattggct acaccaccca taacctggcg ggagtgttga
     3061 acggcgacct ggaagac
//
LOCUS       DATDPQ010000116         6576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3713200, whole genome shotgun sequence.
ACCESSION   DATDPQ010000116 DATDPQ010000000
VERSION     DATDPQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3713200"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VI520_02605"
     CDS             complement(<1..797)
                     /locus_tag="VI520_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36805.1"
                     /translation="MSRIAQQQSPTEVSAAPLFPPSWLDRLQAWIERFPLPPWASYLL
                     AVLGVSLLIHVPLWLDGSLPPGSIEVAQVIAALFLVYFAALIHYLNTTARGALANYRP
                     LLDVKEREYATLEYTLTTIPSRVGLVATLLGALLGAASFFSSPESWGVSPNSSILATT
                     SALLQAMVVVILVTYWAMQVIRQGRTVDRIHRTTTRLNIFRRDPVYAFSALTLRAALG
                     TLLFAYSYPLSVIYLGLPPLSPIDVASLGAAIAISLAIFILPLYRMHR"
     gene            complement(1009..1650)
                     /locus_tag="VI520_02610"
     CDS             complement(1009..1650)
                     /locus_tag="VI520_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLF36806.1"
                     /translation="MRNQTKEHAMSKVIALMSMSLDGYVADLNDGVAEVFDWYFSGDV
                     EIPIGGSDPMTFRVSRPSAEHLHGLISELGAMLTGRRTFDVAQGWGGNHAWGPAFVLT
                     HHIPDGWPRPDSTVHFITDGIETAVNQAKTAAAGKSVGVHGADTIQQCLNAGLLDEIH
                     IDIAAVFLGSGIRLFDHLAGTPIVLGNPTVIQGIGVTHLRYPVRNRPPLTPGA"
     gene            complement(1915..2148)
                     /locus_tag="VI520_02615"
     CDS             complement(1915..2148)
                     /locus_tag="VI520_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36807.1"
                     /translation="MIRLYEKGTNAPLGAITEAQLQFLVDQLEEEWLEDQDYAITPLL
                     LDTFEAEGADPELVSVLRGGLGDREEIEISWSR"
     gene            complement(2874..4013)
                     /locus_tag="VI520_02620"
     CDS             complement(2874..4013)
                     /locus_tag="VI520_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLF36808.1"
                     /translation="MASEVGLNYGDLTWPEVAALPRGTILVLPLGSGYDSEQVVRRIG
                     AGPLCWLPALPFGWPGSSLPVAPEVFERALAGILSAPLEEGFQRIVVLCPSDRIGLPA
                     SVEPIVMPEPAGTGPRSLQLNRERVVLIPVGHTEQHGLHLPMNTDSVIIEAIAHGVVD
                     AIPNDAVALPTLPYGVSTHRKSFAGTFNMGGRAFEDFQLAVIEQLIEAGADRLHLISG
                     HGGNVSFLVNVVKYAGERFPRAFTATASLHTSGKIGVRALEQHRRSKRGGMGHACELE
                     TALMLHLRPDLCHMERAVDETDFIATENYYMDWVEGGELIANPPWEDDTQTGAYGAGT
                     LATAENGVRWLSAAIEEKIAHVREIHEQQDRRLAKRRALKDAGIS"
     gene            complement(4076..5116)
                     /locus_tag="VI520_02625"
     CDS             complement(4076..5116)
                     /locus_tag="VI520_02625"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HLF36809.1"
                     /translation="MVRARGAPRLRGRQIGEAAAPLIQLMLTTYRQLIEASQDRLKLS
                     WAGAVAQSRKYAHYALESYLEELHGMAEGAGAELDDLLALNCMEAIVSDALHLKCTSM
                     ALGPDWTDDRHVLLAHNEDWWPQDKHTLYLIHAEPEHEPPFLALTYGGLLPAIGLNAA
                     GLAQCCDSVYPDDSRVGVPRAFVARSVLGASDLAEAGRRAAHPRREAGYNHLVVNQAG
                     EILSLEASALQHAALPDTGGALVHTNHYLDPAMQLVERDPASLDRSRLRYERATELLH
                     AGRPHSRQSLVRILGDHHNSPSSICSHLQDSQPPLDQYATIASVIIDLTEMTLHVGRG
                     QPCGASFHSYTM"
     gene            complement(5131..5751)
                     /locus_tag="VI520_02630"
     CDS             complement(5131..5751)
                     /locus_tag="VI520_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36810.1"
                     /translation="MRVAIRSPLARLLAALLVAAPLLTACTQDDFAFVQSLAKEWAAG
                     KGLLDSTGKPDYFNIALLLADPSSDPEAAAALQAGMVVKGVEDADRLAQQGAAEGDLT
                     KIDAAIRARPSDWSYQEQKAALLLAQGDAAAAQAANEKSESLVRDRLQAGGNCRTLAR
                     NMFTHRLNALHAQAIRAPSAELIERINQTQAVLDQLIAGQPVAFCP"
     gene            complement(5748..6386)
                     /locus_tag="VI520_02635"
     CDS             complement(5748..6386)
                     /locus_tag="VI520_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36811.1"
                     /translation="MFNVAPQQPQPPPPPQPGAAQPRSRWRRWGCVGCLAFGGLLSCC
                     VLATVFFGPGIWGRLGLFRTSAEDLYSGASDPYASQIVTQILIEAGVPGARAVVVPIK
                     GSDGQIAVITLDSSAGFDGTGTASGNEMVLMDVLQRMREDNRMEMLGIERVSVDYRDK
                     NGGNLLAITVSQESLEAFSQGQLTRKELLQKVDVGLPTLDLQEMLALLAEGS"
BASE COUNT         1274 a         1922 c         2124 g         1256 t
ORIGIN      
        1 cggtgcattc ggtacaaggg aaggatgaaa atggccagag agatcgcgat ggcggcgcca
       61 agggatgcga cgtcgatggg agagagagga gggaggccca aatagatcac ggataagggg
      121 taggaatacg caaaaagcag agtaccaagc gccgcccgaa gggtgagcgc ggagaaggcg
      181 taaacggggt cgcggcggaa gatgttgagg cgagtggtcg tgcgatggat gcggtcgacc
      241 gtacggccct gacggatcac ttgcatagcc caatatgtga cgaggatcac gacgaccatc
      301 gcttgaagga gggctgaagt tgtagctagt attgaggaat tcggcgagac gccccaggac
      361 tcaggcgagg agaagaaact ggccgcgccg aggagggcgc cgagcaaggt ggcaacgagg
      421 ccaacgcggc tgggaatcgt tgttagggtg tactccagtg tggcgtactc tcgctccttc
      481 acatctagga gaggtcgata gttggcaagg gccccacgcg cggttgtatt caagtagtgg
      541 atcagcgctg caaagtagac gagaaagagg gcggcaatga cttgggctac ctcgatcgaa
      601 cccggaggaa gtgagccatc gagccagagc gggacgtgga tcaatagcga cacgcccaga
      661 accgcgagga gataggaggc ccacggcggg agtggaaagc gctcgatcca cgcctggagg
      721 cggtcaagcc agctaggggg gaacagtggg gcagcggaga cttccgttgg agattgctgc
      781 tgagcgatac gtgacatttg tgtcctcgag aactcttcgt gagtggggaa aattctaagt
      841 cgatttcctg ttggtgcggt cgctaattag agcgcggaac acggctgccc aacgcccaag
      901 ctcagccgcc gttttgccgt agcgaagcga aggcaaaacg gtcggctgga gcgccttgtt
      961 gggcctcatg cgacccgttg tggacgatcc gcacctggaa aactacggtt aagcccctgg
     1021 cgttaatgga ggccggttgc gtactgggta gcgcaggtgt gtgacgccga tgccttggat
     1081 gacggtcggg ttgccgagga cgattggggt gccggcgagg tggtcgaaga gtcgaattcc
     1141 ggagccgaga aagaccgcgg cgatgtcgat gtggatttcg tcgaggagtc cagcgttgag
     1201 gcattgctgg atggtgtcgg cgccgtggac tccaacggac ttcccggcag ctgcggtttt
     1261 ggcttggttg acggcggttt cgataccgtc ggtgatgaag tgcacagtcg agtcgggacg
     1321 tggccacccg tcggggatgt ggtgggtgag cacgaatgcc ggtccccagg cgtggtttcc
     1381 gccccatcct tgggcgacgt cgaaggtgcg tcggccggta agcatggcac ccagttcgga
     1441 gatgagaccg tgaaggtgct cggcgctcgg ccgggacacg cgaaacgtca tggggtccga
     1501 tcctccaatg gggatttcaa cgtcgccgga gaagtaccag tcgaacactt cggccacgcc
     1561 atcgttgagg tcggcgacgt agccgtcgag cgacatcgac attaatgcga taactttcga
     1621 cattgcgtgt tccttggttt gattacgcat attacttctc ctcaagtttt tcgggccagg
     1681 cctccggtcg gtcatgaccg gcttatctcc gcccctacct tgtagtcgaa cgagacctcg
     1741 cgaaatcgac attgatgtga gtgcgaagtc tgattgaggc ccaacggcct cgagctgagc
     1801 ggcccaacgg ctcaggctca cgccgcccca ctctcgcgca ccctcgcagg caacgagcca
     1861 ccgcgttttc gccagctagg agggtcggct ccagcgagtg gttaggctgc gccctcatcg
     1921 gctccaagag atttcaatct cctcccgatc tccgagcccc cctcttagta ctgagacaag
     1981 ctccggatct gccccctcgg cctcaaaagt gtcaagtagt agtggcgtta tggcgtagtc
     2041 ttggtcttca agccactctt cctcgagctg atcaactagg aactgaagct gagcttcggt
     2101 aatggccccc agcggcgcat tcgtcccttt ttcgtacaat cgaatcatta ctgctaccct
     2161 ccagcgacga tgtcattttc tagtgcagcc taacggcctc gagctgagcg gcgccgctcg
     2221 gttaccccga actcgaaatc ggcccgtggc ggcgtctgct ccagcgaggg gttagcccgc
     2281 cgccaactcc gggaatcgca acacgcgccg acgtacctgg aaatcgagag gggcttctga
     2341 atttctcctt tcgaggcatt attcgtcgtc cgaaggatgc tcaatctcac cagcgatgaa
     2401 acgatcggga tcgccttgga atgtcctcaa acatgcgcct gtgcagaaat acacgacctc
     2461 cccgcgatag actgagctgg gatactgacc ttggtcgcgt atctcgcttc cacaaactgt
     2521 cttcaacatc ggcttccttg gattctcctc catcagtcat tcaccgcatt ggggttctct
     2581 attgtaatga ttgaaccatg gcaatcggat tggtttcccg cttgagcgtt gaacgcaatc
     2641 gagattgccc aatcactcgg cgggctaact aatacttaga ccacatccac ccagtatagc
     2701 cctgctccca agggtcttat ccagcgaaat ccgggaatat cacccccgcg ggggatgtgt
     2761 aggggatgga tttgtactaa tgtggcggca gagggtgttt atccaaggtg ggccggggcc
     2821 agcgccacaa atcacatcaa ccgcgagggt cccaaacgaa atctgaggtc catctaggat
     2881 atccccgcgt ccttcagggc tcgccgcttc gccagtcgcc tatcctgctg ttcgtgaatt
     2941 tcgcggacgt gggcgatctt ctcttcgatc gcggctgaaa gccagcgcac gccgttctcg
     3001 gccgtggcca gggtgcccgc gccataagcc ccggtctgag tgtcatcttc ccagggagga
     3061 ttcgcaatca gctctccccc ctcgacccaa tccatgtagt aattctcggt ggcgatgaag
     3121 tcggtctcgt ccaccgcccg ctccatgtgg cacaagtcgg ggcggagatg cagcatcaac
     3181 gcggtctcca actcacacgc gtgccccatg ccgccgcgct tcgatcggcg gtgctgctcg
     3241 agggcccgga ccccaatctt tccggaagtg tgaagggaag ctgtcgcagt gaaggccctg
     3301 gggaatcgct cgccggcgta cttgaccacg tttaccagga acgagacatt gcctccatgc
     3361 ccgcttatca agtggaggcg atcggcgccg gcctcgatga gctgctcgat cactgccagc
     3421 tgaaaatcct cgaaggcccg gccgcccatg ttgaacgtgc cggcgaagga ttttcggtgg
     3481 gtgctgaccc cataggggag ggtcggcaac gccaccgcgt cattcgggat tgcgtcaacg
     3541 actccgtggg cgatggcctc gatgatcacg ctgtcggtgt tcatggggag gtgcagtcca
     3601 tgctgctcgg tgtggccaac cgggatgagc acgacccgtt ctcggttgag ctgcagcgag
     3661 cgggggccgg tgcctgcggg ttctggcatc acgattggtt cgaccgaggc cggcagcccg
     3721 attcgatccg acggacacag aactacgatt cgctggaatc cttcctccag cggcgcgctg
     3781 aggattccgg ccagcgctcg ctcaaacact tcaggtgcca ccggcagact gctcccgggc
     3841 cagccgaagg gaagcgccgg tagccagcac agcggcccag ccccgatccg ccgcacgacc
     3901 tgctctgaat cgtagccgga cccgagcggg agcacgagga ttgtgccacg cggtagggcg
     3961 gcgacctccg gccaggtgag gtcgccgtaa ttcaacccta cctcagatgc caatcgaggc
     4021 agcgctccag ccggtgctga gattggatat cgttcgcctc ccgcattggc gcagattaca
     4081 tcgtgtacga gtgaaaagag gcgccgcacg gttggccgcg gccgacgtgc agggtcattt
     4141 cggtaagatc gatgatcacc gaagcgatcg tggcgtattg atcgagcggg ggctgcgaat
     4201 cctggagatg ggaacagatg ctgctcggtg aattgtgatg gtctcccagg atgcggacca
     4261 ggctctggcg ggagtgcggc cgcccggcgt gtaacagctc ggtggcgcgc tcgtagcgca
     4321 ggcgcgaacg atccaggctc gccgggtctc gctcgaccag ctgcatcgca gggtcaaggt
     4381 aatggttggt gtggacgagt gccccgccgg tatcggggag ggcggcgtgc tgcagcgcgg
     4441 aggcttcgag cgagaggatc tcgcccgcct ggttgacgac cagatggttg tatccggcct
     4501 cccggcgcgg atgtgcggcc cggcggcccg cctcggccag atcggaagct cccaggacgg
     4561 accgcgcgac aaacgcccgc ggtacgccaa ctcgggaatc gtcggggtag accgagtcgc
     4621 agcactgtgc gaggccggcc gcgttgagac cgatggcagg cagcagtcct ccataggtca
     4681 gagccaggaa tgggggctca tgctccggtt cggcatggat caagtagagg gtgtgcttgt
     4741 cctgcggcca ccagtcttcg ttgtgagcga gcagcacgtg ccggtcgtcg gtccaatcag
     4801 gaccgagcgc catgctggtg catttcagat gcagcgcgtc actgacgatg gcctccatgc
     4861 agttgagcgc cagcaggtcg tcgagctccg cgccggcccc ctcggccatt ccgtgcagct
     4921 cttccaaata gctttccagt gcgtagtgag cgtacttgcg tgactgagca acagcccccg
     4981 cccagctcag tttcaaacga tcctggctgg cctcaatgag ctgccgatag gttgtgagca
     5041 ttagttgaat caagggagct gcggcctccc cgatctggcg gccgcgcagc cgcggtgcgc
     5101 cgcgggcgcg caccaccagg agctcagtgg ctatggacag aacgcaaccg gctgcccggc
     5161 gatcagctgg tcgagcacgg cctgggtctg gttgatccgc tcgatcagtt cagccgaggg
     5221 ggcccggatg gcctgggcgt ggagcgcgtt gagtcgatgg gtaaacatgt tgcgggccag
     5281 ggtccggcaa ttacctccgg cctggaggcg atcgcgcacc aggctttcag acttctcgtt
     5341 ggcggcttga gcggcggcgg catccccttg ggcaagaagt aaggcggcct tctgctcctg
     5401 gtagctccaa tcgctggggc gggcacggat tgccgcgtca atcttggtca ggtcgccttc
     5461 tgctgcgccc tgttgggcga ggcggtcggc atcttccact cccttgacca ccatccccgc
     5521 ttgcaaggcg gccgcagcct ctggatcgct ggatgggtcc gccaacagca gggcgatgtt
     5581 gaagtaatcg ggttttccgg tggagtcgag tagacccttt ccggctgccc attccttggc
     5641 cagggattgg acgaaagcga agtcatcctg ggtacaagca gtcaacaaag gagccgccac
     5701 cagcagcgcc gccagcaagc gggcgagagg ggatcggata gccaccctca actgccctcc
     5761 gccagtagcg ccagcatctc ttgcaggtcg agcgtgggca ggccgacgtc gaccttttgc
     5821 agcaactcct tgcgggtcag ctgcccttga gaaaaggcct cgagcgattc ctgagaaacg
     5881 gtgatcgcca gcaggttccc gccattctta tcccggtaat cgaccgagac ccgctcgatc
     5941 cccaacattt ccatccggtt gtcttcccgc atgcgctgca gcacgtccat caggaccatc
     6001 tcgttgccgg aggctgtccc ggtcccgtcg aaaccggccg aactatcgag ggtgataacc
     6061 gcgatctggc cgtcactgcc tttgatcggg accacgacgg ctcgggctcc gggaacgccc
     6121 gcctcaatca agatctgggt gacaatctga ctggcatacg gatcggaggc gccggagtac
     6181 agatcttctg cactggttcg gaaaagaccc agccgacccc agattccggg accaaagaac
     6241 accgtggcca gcacgcagca agagagcagt ccgccaaagg ccaggcagcc tacgcatccc
     6301 cagcgacgcc aacgagatcg cggttgggcc gcgcccggtt ggggcggagg gggtggctgg
     6361 ggctgctgtg gagcgacgtt gaacactagc agtttgctta tcgcgggcgg gccagcctgg
     6421 attctaatgc gggcgggggt cttccgacaa gctcggctcg ccgagcctga ggtgcggagc
     6481 gcccgggtcc atggtaaaac ttgggtcgta gacggtttgc tccgcctgca agctagagga
     6541 gaattgagac aatggctatc gatccggtat gtctga
//
LOCUS       DATDPQ010000117         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1358291, whole genome shotgun sequence.
ACCESSION   DATDPQ010000117 DATDPQ010000000
VERSION     DATDPQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358291"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VI520_02640"
     CDS             <1..183
                     /locus_tag="VI520_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HLF36812.1"
                     /translation="HDCTHEGGPLSEGDLQGTVITCPWHGSRFDVTSGKLVRGPTQRP
                     LQTFRVSVEGELARVD"
     gene            183..1643
                     /locus_tag="VI520_02645"
     CDS             183..1643
                     /locus_tag="VI520_02645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLF36813.1"
                     /translation="MMDSVEVLVIGGGPAGVTAALRARELGASVALVERDRMGGTCTN
                     DGCVPTRVLAKAARLRRDAEQGAEYGLLGELPRLDFARLLNRTQRMVYGIQEKKQLIS
                     HLEGSGVQVHAGVGDSRFIDANTLELSDGQTLQAEKFVLCLGGHPRRPTLPGAELGLT
                     HSDTWSLKQLPRSFIVVGGAATGCQVASNFAAFGSEVTILERAPHLLAMEDEAVSEEM
                     REAFERRGIAVMTGIEAVERVEKNAHGLNLLYRSDGRETSLQAEAVLFAIGWVGNVDG
                     LNLEAAGVRVEAGYVPVNEHLQTSAPHIFAAGDITGRMMLVQSAGYEARIAAENAVLG
                     PELRFEHRIVPHGGFTDPEYGSVGLTERTARENGEQIAVAEVSYAELDRAVIDGRPEG
                     LLKLIVSQETHRILGAHAVGEQALEIVQIVSAGMAADMWVEQLAEMELAYPTFTAIVG
                     LAARKVVHQLGVVPLAPEWRALGRPHGAEWERRDGS"
     gene            1681..3288
                     /locus_tag="VI520_02650"
     CDS             1681..3288
                     /locus_tag="VI520_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HLF36814.1"
                     /translation="MSTQNRDLWRILARSGAPGPTAPEPESTPSVEFTPYDPLVAYLQ
                     SARGVVEVDRLDLDSPALRSLKEAGVKLTIPLVAQGELIGMLNLGQRLSEQEYSVDDR
                     TLLSSLATQAAPALRVAQLVRLQKDQARRSERLEQELRIARLIQQTLLPRELPKLPGW
                     EVAAYYRPAREVGGDFYDFIHLEDGTLGVIVADVTDKGVPAALVMATTRSVLHAAAER
                     LISPSQVLARVNNQLIPNMPENMFVTCLYSVLDPMKRTFRFANAGHGLAFRRGAEGVS
                     ELKATGMPLGLMPDMRYEENEVVILPGETILLQSDGLAEAHNSRREMFGFPRVKQLLS
                     DHPSGPAVLIATLVAELERFTAGGWEHEDDVTLLAIECSALGGKAAETARRAGLEVPL
                     PLQADFNIPSRPGNERLAIQKVSEAVQDLGLSSSRLEKLKTAVGEATMNAMEHGNKYQ
                     DDLPVHIRLQASNEKLSVFITDHGGGQRIPGAATPDLEAKLDGLQSPRGWGLFLIQNM
                     VDDLKVTSDETHHTVELMLHLEGGQHG"
     gene            3281..>3508
                     /locus_tag="VI520_02655"
     CDS             3281..>3508
                     /locus_tag="VI520_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLF36815.1"
                     /translation="MAEAQLEANVRLQAGMAIIDLRGDIDGFAEAALIAAYLQAETKE
                     PTALALNFSQVDYINSTGIALIVSLLARARKD"
BASE COUNT          657 a         1072 c         1182 g          597 t
ORIGIN      
        1 cacgactgca cccacgaggg cggcccgctg agcgagggcg acctgcaggg gacggtgatc
       61 acgtgcccgt ggcatggctc ccgttttgac gtcaccagcg gaaagctcgt ccgtggaccc
      121 acccagcggc cgctgcaaac cttccgggtg tcggtggaag gcgaactcgc gcgggtcgac
      181 tgatgatgga tagcgtcgag gtcctggtga tcggaggcgg gccggcgggg gtgaccgccg
      241 cgctgcgggc ccgcgagctg ggcgcctcgg tggcgctggt agaacgcgat cgcatgggcg
      301 gcacgtgcac caacgacggt tgcgtcccca cccgggtgct ggccaaggcc gcccggctgc
      361 gccgtgatgc agaacagggc gcggagtatg gtctgctcgg ggaactcccc cgactggact
      421 tcgcccgcct gctcaaccgc acccaacgga tggtctacgg tattcaggag aagaagcagt
      481 tgatcagcca tttggaaggc tctggcgttc aggtccacgc cggagtagga gactcgcgct
      541 tcatcgatgc aaacaccctg gagctgagtg acgggcagac actgcaggcg gagaagtttg
      601 ttctgtgcct cggaggccat ccccggcgcc ccactctccc cggggccgag cttggtttaa
      661 cccacagcga cacctggtcg ttgaaacagc tgccgcgttc gtttatcgta gtcggcggag
      721 cagccactgg gtgccaggta gcctccaact tcgccgcttt tggttccgag gtgacgattc
      781 tggagcgtgc tccccacctg ttggcgatgg aggacgaagc cgtttccgaa gagatgagag
      841 aggccttcga gagacgaggg attgcagtaa tgaccggcat cgaagccgtc gaaagggtcg
      901 agaagaacgc ccacggcctc aatctgctct accgctcaga tggccgcgag acatcacttc
      961 aggccgaggc ggtgctattt gcaatcgggt gggttgggaa cgtggacgga ctaaacctgg
     1021 aagccgccgg ggtgcgggtc gaggccggct acgtcccggt gaatgaacac ctgcagacct
     1081 ctgccccgca catcttcgcc gcgggagaca tcaccggccg gatgatgctg gtgcagagcg
     1141 ctggatacga ggcacgcatc gcggccgaga acgccgtgct tgggcctgag ctgcgctttg
     1201 agcaccggat cgtcccccat ggaggcttca ccgatcctga atatgggagt gttgggctga
     1261 ccgagcggac cgcgcgtgaa aacggtgagc agattgccgt ggcggaggtt tcctacgccg
     1321 agctcgaccg ggcggtcatc gacggccgac cggagggttt gctcaaactg atcgtctcgc
     1381 aggagacaca ccggattcta ggggcacacg cggtgggcga gcaggcgctg gagatcgtgc
     1441 agatcgtgtc ggctggtatg gcggccgata tgtgggtgga gcagctggcc gagatggaac
     1501 tggcctaccc gacgttcacc gccatcgtcg gcctggcagc cagaaaggtc gttcaccagc
     1561 taggcgtggt ccccttggcg ccggagtggc gagcgctggg ccggccgcac ggagcggaat
     1621 gggagagaag agatgggtcg taacgctttt gggactctgc ggatggatac tggaggctag
     1681 atgagcactc agaatcggga cctctggcgc atcctggccc gatccggggc accgggcccg
     1741 actgctcccg agccagaatc cacgcccagc gttgaattca cgccatatga cccactcgtc
     1801 gcctatctac agtcggcgcg gggtgtggtg gaggtcgatc ggctcgatct ggattcgccg
     1861 gccctgcgct cgctgaagga agcgggcgtc aagctgacca ttccgctcgt cgcccaaggc
     1921 gagttgatcg gcatgctcaa cctggggcag cggctgagcg agcaggagta ctcggtggac
     1981 gaccggaccc tactgagcag cctcgctacc caggccgcgc cggcgctgcg ggtggcccag
     2041 ctggtccggc tgcagaagga ccaggcccgt cggagcgagc ggctcgaaca ggagctgcgg
     2101 atcgcccggc tgattcagca gaccctgctg ccccgcgagc tgccaaagct ccccggatgg
     2161 gaggtggccg cctattaccg tcctgcccgg gaagtggggg gcgatttcta cgactttatc
     2221 catctggagg atggcacgct aggcgtaatc gtcgccgacg tcacggacaa aggagtgccg
     2281 gccgcccttg tcatggccac cactcgcagt gtattgcacg ccgccgccga gcgcttgatc
     2341 tccccctctc aggtgttggc gcgggtaaac aaccagctca ttccgaacat gcccgagaac
     2401 atgtttgtca cctgccttta ttcggtgctg gacccaatga agcggacctt ccgcttcgcg
     2461 aacgccgggc atgggctggc cttccggcgc ggggccgagg gcgtctccga gctcaaggcc
     2521 accggcatgc ccctcgggtt gatgccggat atgcgctacg aagagaacga ggtcgtcatc
     2581 ttgcccgggg agaccatcct gctccaaagc gatgggttgg cggaggccca caactcccgc
     2641 cgcgagatgt tcggcttccc gcgggtaaag cagctcctga gcgaccaccc aagcggccct
     2701 gctgtgttga tcgccacgct ggtggctgag ctggagcgct tcacggctgg cggatgggaa
     2761 cacgaggacg atgtcacctt gctggcgatt gagtgctccg cactcggtgg gaaggccgcc
     2821 gaaacggccc gtcgtgcagg gctagaagtg ccgctgccgc tacaggccga tttcaacatt
     2881 cccagccggc cggggaacga gcggcttgcc atccagaaag tgagcgaggc cgttcaagac
     2941 ctcggtcttt cgagcagccg gcttgagaag ctgaagaccg cggttggcga ggcaaccatg
     3001 aacgccatgg agcacggcaa caaataccag gacgaccttc cggtccatat ccgtctgcag
     3061 gcctcgaatg agaaactctc ggtcttcatc accgaccacg gcgggggtca gcgaatcccg
     3121 ggggccgcaa cccccgacct ggaggctaag ctggatgggc tgcagagccc ccggggctgg
     3181 ggactgttcc tgatccagaa catggtagac gacctgaaag tcacctcaga cgaaacgcat
     3241 cacactgtgg aacttatgct ccacttggaa ggaggccaac atggctgagg ctcagctgga
     3301 ggccaatgtc cggctgcaag ccggaatggc catcatcgat ctacgcgggg acatcgacgg
     3361 gttcgccgag gccgctttga tcgccgccta cttgcaggca gagacgaagg agccgacggc
     3421 cctggcactg aacttcagcc aagtggatta catcaacagc accggcatcg ctctgatcgt
     3481 cagtttgctt gcccgggccc gcaaggac
//
LOCUS       DATDPQ010000118         7283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2398405, whole genome shotgun sequence.
ACCESSION   DATDPQ010000118 DATDPQ010000000
VERSION     DATDPQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398405"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VI520_02660"
     CDS             complement(<1..878)
                     /locus_tag="VI520_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF36816.1"
                     /translation="MSVAEERTATAFANRPPGSAPRCSVVIRAYNEEQHIERLLAGIL
                     RQSIDGVEILLVDSGSTDSTVAIARRFPVNVLSIDPADFTFGRSLNLGLRHASAQQVV
                     FASAHVYPVYPDWLERLLEPFADPRVALVYGKQRGNHSTHFSEQQQFAKLYPETSVVP
                     QEHAFCNNANAAVRRDLWLQRAYDEELPGLEDLEWALWARSEGYQLAYVAEAEVIHVH
                     DETPRQVYNRYRREAIALKRLRPHEHFGLADFIRLYASNVVSDARHALRQRQFAQAWA
                     SLLWYRLMQFWGTYRG"
     gene            complement(883..2562)
                     /locus_tag="VI520_02665"
     CDS             complement(883..2562)
                     /locus_tag="VI520_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36817.1"
                     /translation="MNLQRVSLRIERAGADRTIRRQVNRVERYLARTNQLASSIGSHP
                     EAVEGSHPEPVEGSVLFFNASTRIHTLSLNAAFSLLASWSVRAAGVPVRHVVCRAGME
                     QCILGTHSVGQQRPPPCGPCTRFSDMLFPEELVIPLPKSGNSGEELGGPSLESLVQFQ
                     HREYPLGELCLPGLRWALRRHHLADDPPTRQLLGQYLRSAVHLVDHLDRILENSRPRV
                     LVVFNGIMYPEAVARAVALRHRIPVVTHEVGLRPFSGFFSHDHATFREVDLPTQEAFE
                     SDWEKQVDAYLELRRRGQFSMAGIEFWPQIKSLPRQLTNALETHRQMIPIFTNVVFDT
                     SQSHSNTLFEDMFEWLEALVGVIISRPETLFVIRAHPDEDRPGKASAESVADWFRRSP
                     LNGRENVILIGPSEFTSSYELIDRAKCVLVYSSSVGLEASVLSVPVLCAGRARYTQAQ
                     TVVFPTSRQAYLDQLDRFVAAERLEVSEQTRQNAKAFLYHELYNASLDLAEFLLPYRS
                     MPGMVVFSDFDPRSLEDHPTLAVIRDGVLEGSPFLLPARSVRIPARAEAVP"
     gene            complement(2578..3453)
                     /locus_tag="VI520_02670"
     CDS             complement(2578..3453)
                     /locus_tag="VI520_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HLF36818.1"
                     /translation="MPREIRLGDRLVGEGHPTYIVAEIGINHNGEVEIAKRLIDAAML
                     AGVDAVKFQKRTPELCVPPEQRDIMRETPWGYISYLEYRNQVEFGEPEYAAIDAYCKE
                     RKLTWFVSVWDEPSVDFMESYNPVCYKIPSASLTDHALLRRVRGTGRPAILSTGMSTM
                     EQIEAAVEVLGTANLLINHATSTYPCEPEELNLLTLQTLQERFPCPIGYSGHEVGLIP
                     SVVAVALGACLLERHITLDRAMWGSDQAASVEPGGIQRLVKYIRVTEKALGDGKKRVY
                     PSELDSLRKLRRVHA"
     gene            complement(3446..4681)
                     /locus_tag="VI520_02675"
     CDS             complement(3446..4681)
                     /locus_tag="VI520_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HLF36819.1"
                     /translation="MSPTEVLAVIPARGGSKSIPRKNIEPFGGHPLLAYSIAAGLQAR
                     SVSRVIVSTEDPEIAERAVQYGAEIPFLRPTELAQDDTPDLPVFRHALGWLAEHESYH
                     PGIVVQLRPTSPVRPRNCVDRAVEILVNSPEADSVRSVVASGQNPYKMWQIDEQGVLR
                     PLLQAGPPEAYNAPRQSLPQTYWQTGHIDALRSRTISEMGSMSGNTILPLVLDASFAV
                     DIDTTRDWAWAERLVQAGELDMVRPGPVPRPLPEKVELLVLDFDGVLTDDRVWVSEQG
                     LESVAANRKDGYGIARLKEHGVEVIVLTRETNPVVAARCQKLGIPAIQGVVDKGAKLR
                     ELLQARELEPLRTVYLGNDLNDLPCFPLVGCAVAVADAHPTVRAAADLRLTQRGGRGA
                     VRELCEMLLEKMTREVSHA"
     gene            complement(4678..5535)
                     /locus_tag="VI520_02680"
     CDS             complement(4678..5535)
                     /locus_tag="VI520_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF36820.1"
                     /translation="MADQSVLAVMKPGQALKRFDLNGQVAIITGGAGLLGRSHAITLA
                     SAGAAVVLADIDGEGARRVAEEVQTSGARDCLPQTTDVSDPESVRIMAKAALERFGRI
                     DILVNNAALDPKFDPKHASEHGQRFENFPFRTWQETLAVNVTGMFLCAQAVAPTMTEA
                     QRGVIVNISSTYGMVGPDQRLYQRDSSPPQFKPVTYTVSKAAALGLTRYLATYFAGTG
                     IRVNSLTPGGVYADHDEEFVQRYSAKTVLGRMAEKEEISSALLFLVSDASSYMTGANL
                     VVDGGWTAW"
     gene            complement(5564..6577)
                     /locus_tag="VI520_02685"
     CDS             complement(5564..6577)
                     /locus_tag="VI520_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLF36821.1"
                     /translation="MRFLICGLGSIGRRHLKNLLALGERDIVLYRTGLSTIGDAELAG
                     LPVEHDLETALERWRPDAALVTNPTGLHLRVAVPAAQAGCHLLIEKPVSDRLEGLDDL
                     RRAIADGGGQILMGFQFRFNQGLIRAKDLIAKGTIGRPLSARAHWGEYLPDWHPWEDY
                     RSSYAARADLGGGAALTLCHPFDYLRWLLGEVISVQGATLRSGTLDLDVEDLAEAILQ
                     LDGGCLASVHLDYYQRPAQHWLAIQGAEGTLRWDGSPGDLQWRSAADSDWHAEPAAEG
                     YERNTMFLEEMKHFLAVARGDQQPLCSLDDGVRALEIALAVRRSAQEGMRIELRTPEW
                     SAA"
     gene            complement(6582..7229)
                     /locus_tag="VI520_02690"
     CDS             complement(6582..7229)
                     /locus_tag="VI520_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HLF36822.1"
                     /translation="MQFYGKKILFLGAHPDDIELGCGALLAHISGKAELLCVTLSDNQ
                     SNPKLKNLIEEHYASMAILGVPRERVLVKNFETRNFPRDRQEILEYLYELSREHRPDL
                     VFVHSSADIHQDHRVVTEEALRAFRGVSVLGFDVFRSSFDFFPHFLVEVEEQDVERKL
                     NALAAYETYADKYYFDPEVMRAALVRNGALAERPYAEGFDILRIVASYDMGSASG"
BASE COUNT         1394 a         2362 c         2174 g         1353 t
ORIGIN      
        1 aagccccgat acgttcccca gaactgcatc aggcgatacc agagcaggct ggcccaagcc
       61 tgagcgaatt gccgctgccg cagggcatgg cgggcgtcgc tcacgacatt ggaagcatat
      121 agccgaatga agtcggccag accgaaatgc tcgtgcggcc gcagccgttt caaggcgatc
      181 gcttcgcgcc ggtaccggtt gtagacctgg cggggggtct catcgtgcac gtggataacc
      241 tccgcctctg ccacgtaggc cagctgatac ccctcgctcc tggcccacaa ggcccactcg
      301 aggtcctcca gtccgggcaa ctcctcgtcg taggcccgct gcagccacag gtcgcggcgg
      361 accgccgcgt tggcattgtt gcaaaaggca tgctcctgcg ggactaccga agtctcggga
      421 tagagcttgg caaactgctg ctgttcgctg aagtgtgtgg agtggttgcc acgctgcttt
      481 ccatacacca atgcaacccg tgggtcggcg aacggttcga gcagccgctc cagccagtcc
      541 ggatagaccg gatacacgtg agcgctggcg aagacgacct gctgcgcgct ggcatgacgg
      601 aggcctaggt tcaatgagcg cccgaaggtg aaatcggccg ggtcgatcga taacacgttc
      661 accggaaagc gccgggcgat cgcgacggtg gaatcggtcg agcccgaatc gaccaacagg
      721 atctcaacgc cgtcaatcga ctgccgaagg atgccggcaa gcaagcgctc gatgtgctgc
      781 tcttcgttgt aggcccggat gaccaccgag cagcggggcg cggatcccgg cggccgattc
      841 gcaaaggcag tggcggttcg ttcctcagct acagacatgc gactatggta ccgcctccgc
      901 ccgggccggg atccgcaccg atctggccgg tagcagaaag gggctccctt ccaacacgcc
      961 gtcccgaatc acggcaagcg tcggatggtc ttccaagctc cgcgggtcaa aatcggagaa
     1021 tacaaccatg cccggcatgc tgcgatatgg cagcaggaat tccgcgagat ccaacgaggc
     1081 gttgtatagc tcgtgataga gaaaggcctt cgcgttctgg cgggtctgct ctgagacctc
     1141 cagacgctcc gctgccacaa atcgatcgag ctgatcgagg taggcctggc ggctggtggg
     1201 aaagacgacc gtctgcgcct gggtgtaacg ggcccgaccc gcgcacagca ccggaacact
     1261 gagcacggag gcctccaatc cgactgaaga gctatagacc aatacgcact tggcgcgatc
     1321 gatcaattca taagagctgg tgaattccga agggccaatc aagatcacgt tctcccgccc
     1381 gttcaggggg ctgcggcgga accagtcggc cacgctttcg gcgctggcct ttcccggccg
     1441 gtcctcatca gggtgggccc gaatcacaaa gagtgtctcg ggccgggaga tgatcacgcc
     1501 gacaagcgcc tctagccact caaacatgtc ctcaaacagt gtgttggaat gggactgact
     1561 ggtgtcgaaa accacgtttg tgaagatcgg gatcatttgg cggtgcgtct ccagggcgtt
     1621 ggtgagctgc cttggaagag acttgatctg aggccagaac tcgatccctg ccatggagaa
     1681 ctgccctcga cggcgcagct cgaggtaggc gtcgacttgc ttttcccagt cggactcgaa
     1741 cgcctcctgg gtaggcagat ccacctctcg gaaggtcgcg tggtcgtggg agaagaagcc
     1801 ggaaaagggt cgcagcccga cctcgtgggt cacgaccggg attcggtgcc ttagagccac
     1861 cgcccgcgcg actgcctccg gatacatgat cccgttgaac accaccaata cgcgcgggcg
     1921 actgttttcc agaatccggt caaggtggtc aaccaggtgc accgccgatc tcagatattg
     1981 tccaagaagt tgacgagtcg gcggatcgtc ggccagatga tgcctgcgaa gtgcccagcg
     2041 aaggccgggc aagcacagtt cccccagtgg atactcacgg tgctgaaact ggaccagcga
     2101 ctccaagctc ggtccgccaa gctcctcccc tgagttgcct gactttggca aagggatgac
     2161 caactcctca ggaaagagca tgtcactgaa ccgggtgcac ggcccgcatg gcggggggcg
     2221 ctgctgacct accgagtggg tcccaagaat gcactgctcc atgccggctc ggcagaccac
     2281 atgccgcacg ggcacgccag ccgcccgcac agaccaggag gccagcagcg agaatgcggc
     2341 gttcaggctg agcgtgtgga tgcgagtgga ggcgttgaag aagagaaccg acccttcgac
     2401 tggctcaggg tgcgaccctt caacggcctc agggtgcgac cctatgctag aggccagctg
     2461 attggtcctc gccaagtacc gctcgactcg gttgacttgc cggcggatcg tgcggtccgc
     2521 cccggctcgc tcaatgcgaa gtgagactcg ttgcaggttc actaggggta cgagggatca
     2581 ggcatgcaca cggcgcaact tgcggagcga atccaattcg ctcgggtaca ctcgtttctt
     2641 gccgtcgccc aatgccttct ccgtgactcg gatgtatttc actaaccgct ggataccgcc
     2701 cggctccacc gaggcggcct gatcgcttcc ccacatggcc cgatcgagcg taatgtgccg
     2761 ctccagcagg caggccccca gtgccacagc tacaaccgac gggatcagtc cgacctcatg
     2821 tcctgaatag ccgatcggac acgggaaccg ctcctgcagc gtctgcagcg tcagcaggtt
     2881 gagctcttcc ggctcgcagg ggtaggtgct ggtggcatgg ttgatgagca gattcgcggt
     2941 ccccagcacc tcaactgccg cctcgatctg ctccattgtc gacattccgg tggagaggat
     3001 ggcaggcctt ccggtgcctc gcacccgccg caacagggca tggtcggtca gtgaggcgga
     3061 ggggatcttg tagcacaccg gattgtagga ctccatgaaa tccaccgacg gctcgtccca
     3121 gacagagacg aaccaggtca gcttccgttc cttgcaataa gcatcgatcg cggcgtactc
     3181 tggttcgcca aactcgacct ggttgcggta ctcgaggtaa gagatgtatc cccaaggtgt
     3241 ctcgcgcatg atgtcgcgct gctccggggg aacgcacagc tcgggggtac gcttctgaaa
     3301 ctttactgcg tccacgccgg ccagcatcgc ggcgtcgatc agccgcttgg cgatctccac
     3361 ctcgccgttg tgattgatcc cgatctccgc cactatgtag gtgggatggc cctcccccac
     3421 caggcggtcg cccaagcgga tctctctagg catgcgacac ctctcgcgtc atcttctcca
     3481 gcagcatctc gcagagttca cgcacggcgc cgcgtccacc gcgctgagtg aggcgcagat
     3541 ccgccgcggc acgtaccgtg gggtgggcgt ccgccaccgc caccgcgcac ccgaccagcg
     3601 ggaagcacgg caggtcgttg aggtcattgc caaggtagac cgtgcgcaac ggctccagct
     3661 cgcgcgcctg cagcagctcg cgcaacttcg cacctttgtc caccacccct tgaatcgcgg
     3721 ggatcccgag cttctggcag cgcgccgcta cgaccgggtt ggtctcccgg gtcagcacga
     3781 tgacctctac cccatgctcc ttgagccggg cgatgccgta gccgtccttg cgattggccg
     3841 ccacgctctc caacccctgc tcgctcaccc acacccggtc gtcggtgagc acgccatcaa
     3901 agtccaagac caacagctcg actttctccg gaagtgggcg cggcaccggc cccgggcgga
     3961 ccatgtccag ctcgccggct tggaccaaac gctcggccca ggcccagtcg cgggtagtgt
     4021 cgatgtcgac cgcaaagctc gcgtccagta cgagcggcag gattgtattc ccgctcatcg
     4081 agcccatttc ggatatcgtc ctggatcgga gcgcatcgat atggccggtc tgccagtacg
     4141 tctgcgggag tgattggcgc ggcgcgttat aggcttctgg tggcccagct tgaagcaggg
     4201 gtcggagcac accttgttca tcgatctgcc acatcttgta tgggttctgc ccagaggcga
     4261 ccacgcttcg aaccgagtca gcctccggcg agttcaccag tatctcaacc gccctatcga
     4321 cgcagttgcg cggccgcacc ggcgaggttg ggcgcagctg gaccacgatc cctggatggt
     4381 aactctcatg ctcggcgagc cagcccagcg cgtggcgaaa cacgggcaga tccggcgtgt
     4441 catcctgcgc aagttccgtc ggccgcagga agggaatttc ggcaccgtac tgcacggccc
     4501 gttcggcgat ctccgggtcc tcggtcgaga cgatcacccg cgagacgctg cgcgcctgca
     4561 aacctgcagc gatgctgtaa gccagcagcg gatgaccacc gaagggctcg atgtttttgc
     4621 gcgggatcga cttggagccg cctcgcgccg gtatcaccgc cagcacttcc gttgggctca
     4681 ccaggccgtc cagcctccgt ccaccaccag gttagcgccg gtcatgtagg aggaggcgtc
     4741 cgagaccagg aacagcagcg cgctgctgat ctcctccttt tcagccatac gccccaggac
     4801 tgttttggcc gaatagcgct gaacgaattc ctcatcgtga tcggcgtaca cccctccggg
     4861 tgtcaggctg tttactcgga tcccggtccc ggcaaagtag gtggcgagat agcgggtgag
     4921 gccgagcgcg gccgctttcg agaccgtgta ggtcacgggt ttgaattgcg gtggcgagct
     4981 gtccctctga tacagccgtt ggtcgggtcc aaccatccca taggtcgaag agatgttcac
     5041 gatcacgccc cgctgagcct cggtcattgt tggcgccact gcctgagcgc acaagaacat
     5101 gccggtcacg ttgacggcca gagtctcctg ccaggttcta aatggaaagt tctcgaagcg
     5161 ttggccgtgc tcactggcat gcttcggatc gaacttggga tctaaggcag cgttgttcac
     5221 caagatgtca attcgcccga agcgctccag cgccgcttta gccattatcc tgactgactc
     5281 agggtcggaa acatcggttg tctgtggaag gcagtccctt gcccctgagg tttggacttc
     5341 ctcagcgact cgccgcgcgc cttcaccgtc gatgtcggcc agcaccaccg ccgcgccggc
     5401 ggaggccagc gtgatggcat gcgatcggcc aagcaagccc gcaccgccgg tgataatcgc
     5461 cacctggccg ttcaaatcaa accgcttcag cgcctgacct ggtttcatca cagccagcac
     5521 actctgatca gccatgccgg tcgaaggaac tcatgcgatc tcctcaggcc gcgctccatt
     5581 ctggcgtgcg aagctcaatt cgcatgccct cttgcgcgga tcggcgaaca gccagggcaa
     5641 tctccagcgc ccgcactccg tcatccaagg aacagagcgg ctgctgatcg cctcgggcga
     5701 ccgcaagaaa atgcttcatc tcttcaagga acatcgtgtt ccgctcatag ccttcggccg
     5761 ccggctcggc gtgccagtcg ctatccgcag cagacctcca ctgcaaatcc ccaggacttc
     5821 catcccagcg gagcgttccc tcggccccct ggattgccag ccagtgctga gccggtcgtt
     5881 ggtagtagtc cagatggaca ctcgccagac agcctccgtc caactgaaga atcgcttcgg
     5941 ccaggtcctc tacatcaaga tccaatgtcc cgctgcgaag ggtcgctccc tgtacagaga
     6001 tcacttcccc caacaaccac cgtaggtagt cgaaaggatg gcagagggtc agggccgcgc
     6061 cgccccccaa atcggcgcga gccgcgtagg agctccgata gtcttcccac gggtgccagt
     6121 cgggcaggta ttctccccag tgggctcgag cgctgagcgg ccgaccgatg gtccccttcg
     6181 cgatcaagtc ctttgcgcgg atcagcccct gattgaaccg gaactggaag cccatcagaa
     6241 tctggcctcc gccatccgcg attgccctac gcaggtcatc caatccctcg agccgatccg
     6301 agaccggttt ttcgatcagc aggtggcagc cggcctgtgc cgcgggcacc gcgacccgca
     6361 gatgcagccc ggttggattg gtcaccagcg ccgcgtctgg ccgccagcgc tcgagcgcgg
     6421 tctccagatc gtgctcgacc ggcagccctg ccagctcggc atcccctatc gtgctcaggc
     6481 ccgtccggta cagcacgatg tcccgctcgc cgagcgccag caggttcttc agatgccgcc
     6541 ggccgatgga gcccaatcca cagatcaaga aacgcatcgg gctacccaga cgccgatccc
     6601 atgtcgtagc tcgccacaat tctcagaatg tcgaatccct ccgcgtacgg tcgctccgcc
     6661 agcgcgccgt tgcggaccag cgcggcccgc atcacctccg ggtcgaagta atacttgtcg
     6721 gcgtaggtct catatgccgc gagcgcgttc agtttccgct ccacgtcttg ctcttccact
     6781 tcaaccagaa agtgagggaa aaagtcgaag ctgctgcgga agacatcaaa tcccaggacc
     6841 gacacgccgc gaaaggcccg cagagcttcc tcggtcacga cccggtggtc ttggtggatg
     6901 tccgccgagc tgtggacgaa caccaagtcg gggcgatgct ctcggctaag ctcgtagaga
     6961 tactccagga tttcctgccg atcgcgggga aagttgcggg tctcgaagtt cttcacgagc
     7021 actctttccc ttggcactcc caggattgcc atgctggcgt agtgctcctc gatcaggttc
     7081 ttgagctttg ggttgctctg gttgtccgac agggtcacgc acaacagctc ggccttgccg
     7141 gagatgtgtg ccagcagcgc gccacatcct aattcgatat cgtccggatg ggcgcccaag
     7201 aacaggatct tctttccata gaactgcatg cgattgttcc tcgaagtcga agcgcctcag
     7261 ccgattagca tcggacggaa tat
//
LOCUS       DATDPQ010000119         6733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1872535, whole genome shotgun sequence.
ACCESSION   DATDPQ010000119 DATDPQ010000000
VERSION     DATDPQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872535"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VI520_02695"
     CDS             <1..279
                     /locus_tag="VI520_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLF36823.1"
                     /translation="EAALIAAYLQAETKEPTALALNFSQVDYINSTGIALIVSLLARA
                     RKDKRPVIAFGLSDHFAEIFEITRLSDFMRIVSDEAAARLGTAEEKSQ"
     gene            276..635
                     /locus_tag="VI520_02700"
     CDS             276..635
                     /locus_tag="VI520_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLF36824.1"
                     /translation="MSDTKVAATVRNAGEAASIVDIQGEITSFAEGVLMDAYKQASGR
                     EANAIILNFRGLEYMNSGGIGLLVTMLIRANRHNHKLLAFGLTDHYQQIFELTRLNEA
                     IQIFQTEAQALAAVAGR"
     gene            665..1420
                     /locus_tag="VI520_02705"
     CDS             665..1420
                     /locus_tag="VI520_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36825.1"
                     /translation="MQTETQKPESRDAAYWAGPVSKLKVTDVPSGAVSMNVEGRHVVG
                     PLQGFGQLWQKTYRVRLSSADIGPTTVVSFLKEKLPELMPRDARFYPSVSGVKPGELV
                     LINATLPGIPGGIATGVMVLYSDDESFTLMCPEGHPEAGWNTFSAYIEGDTTVVQIQS
                     LARANDPVFEFGFRFMGGAAQQERTWHHVLTGLAKHYGKDAPVQMIRALVDGKLQWSQ
                     AKNVWYNSAIRSVLNAPVRLVRRLIGRGGRASA"
     gene            1453..2886
                     /locus_tag="VI520_02710"
     CDS             1453..2886
                     /locus_tag="VI520_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLF36826.1"
                     /translation="MRRPEVDAVVIGAGPNGLAAAITLAQAGRSVTVIEAADTIGGGA
                     RTAELTLPGFHHDVCSAIHPLGLASPFFRAVPLHEHGLKWIQPPGALAHPLDDGTAVL
                     VSQDLRATAGQLGQDGQAYLNLLGPLVENWQALLAEILGPFRLPRRPFLMARFGRLAI
                     QSARGLATARFRGPRARALFAGLGAHSMLPLESPVSAAAGLVEGTLAHAVGWPMAAGG
                     SQRITDALVGLLRSLGGQIVTGRRVTSLDELPSARTVFFNTSPKELIRIAGQRLPAGY
                     RRRLERFRYGPGVFKLDWALDGPIPWTAEACRQAATLHLGGKLEEIAEAEAAVARGEH
                     PERPFIIFAQQSLFDQDRAPQGKHTAWGYCHVPNGSTVDMTERIEAQIERFAPGFRNR
                     ILARHSRNSVEMEKYNSNYVGGDINAGIQDLRQIWTRPVARLSPYTTPVAGLYLCSSS
                     TPPGGGVHGMCGYHAARAALHRSLKSG"
     gene            complement(3027..3689)
                     /locus_tag="VI520_02715"
     CDS             complement(3027..3689)
                     /locus_tag="VI520_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLF36827.1"
                     /translation="MAEEWPLRHDLVEVAQRCEAYGLVLRSQGNFSARIPESDRILIT
                     PTGIPYSTMRPGDIVEVGLDGRVLKALHPPSYELPVHLAAYRADPRIKACAHTEPPHI
                     NALYALNKSLPNVLGNFIYAFAGRGLAVGPRMKSGTSAFATANIEALGDRFGIVWPNH
                     GLFCVGRTLEVAFIRSLHAEQAAQVYYLAIALNEREPLLLPNEEQRQYVQAAQADGWD
                     AP"
     gene            3841..4800
                     /locus_tag="VI520_02720"
     CDS             3841..4800
                     /locus_tag="VI520_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLF36828.1"
                     /translation="MKRWVIGADLGGTLIRAIRTDLEGRKVARAEIQTLAQEDSEAIL
                     GRIFQMIQQASEGADPSEVLGIAIGAPGPIDELGRLHDPPNLPGMKEFSLGEQIQDRF
                     QIPSYAGNDGNLAAFGELRFGAGRGLEDLIYMSIGTGVGGGIICDGKLLLGAHGFAAE
                     IGHQTLVPDGPLCACGQPGHLEALASGPSIARQAKERLQAGASSTIDHYREAGNELTG
                     KSVTDAARHGDELALELLTEAATYIGLGLVNLIHILEPQRILLGGGVSQAGELLFEPI
                     RQTVHDRVMSPVFRGVEIQPAALGKDVGLMGAVAWVLERSSPD"
     gene            5046..>6733
                     /locus_tag="VI520_02725"
     CDS             5046..>6733
                     /locus_tag="VI520_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36829.1"
                     /translation="MTAEVAPRDLRNTLATRRLVGLWRLADGFRQLLLLATLGIGLAA
                     LARAASYLLVGHFVDVVLLREDLGRGLPWVAAGFIGLALGQGGLTYLSGRLAARSAEG
                     ITKRLREYLFDHLQRLSFAYHAQARSGDLIQRSTSDVDAIRRFFADQAINAGRILLMF
                     LVNLAVLIQLDAQLALLSVVVVPVTVTMSYLFFRRISKAYEDYQEQEAVLSAALQENL
                     AGVRVVKAFARQEFERSKFDTENMEKFRRGRRLLIMHSLHWPISDILSGAQTLFGLTL
                     GALMVMDGRISIGTYLAYSGLIIWIIWPIRNLGRLVVDMSSGFGSYGRVAEIIREERE
                     PLDEGSFRPSGPPRGELVFEGVGFAYPDGGGEVLEDIHLHVLPGQVVALLGPTGSGKT
                     SLVNLLPRFYDYTSGNLLLDGVELKDYPRRYLRQHVGIVEQEPFLFSRTIRENIAYGM
                     GREVSPAEIEAAARAAAIHEGILAFPDGYDTLVGERGVTLSGGQKQRVAIARTLLIDP
                     RILILDDSTSSVDTETEALIRSALERLMQGRTSFIIAHRVQSLMIADLILVLQGG"
BASE COUNT         1216 a         2097 c         2172 g         1248 t
ORIGIN      
        1 gaggccgctt tgatcgccgc ctacttgcag gcagagacga aggagccgac ggccctggca
       61 ctgaacttca gccaagtgga ttacatcaac agcaccggca tcgctctgat cgtcagtttg
      121 cttgcccggg cccgcaagga caagcgcccg gtcatcgctt ttgggctgag cgatcatttt
      181 gccgagattt tcgagatcac ccgattgtcg gatttcatgc ggatcgtatc cgacgaggcc
      241 gccgcccgtc tgggcactgc agaggagaag agccagtgag cgatacaaaa gtagcagcca
      301 cggttcgcaa tgccggagag gcggccagca tcgtggacat ccagggagaa atcacttcct
      361 tcgccgaagg tgttttgatg gacgcctata agcaagccag cggcagggag gcgaatgcga
      421 tcatcctgaa tttccggggt ctggagtaca tgaacagcgg ggggatcggg ctgctggtca
      481 ccatgctgat ccgggccaac cggcacaatc acaaactgct ggccttcggg ttgacggatc
      541 attatcagca gatcttcgag ctcacccgcc tgaacgaggc gatccagatc ttccaaaccg
      601 aggcgcaggc tctggccgcg gttgccggcc gctgaaccca agtcagaaga agggatgagc
      661 agctatgcaa actgagacac agaaacccga atcgcgcgat gcggcctatt gggcggggcc
      721 ggtctcgaag ctcaaagtga cagacgtccc cagcggggcc gtaagcatga atgtagaggg
      781 ccgccacgta gtcggtcctc tgcagggttt tggtcagctg tggcaaaaga cgtatcgggt
      841 gcggcttagc agtgcagaca ttggccccac gacggtcgta agtttcttga aggaaaaact
      901 gccggaactc atgccgcggg atgcccgctt ctacccctcg gtatcaggcg tgaagcccgg
      961 agaattagtg ctgatcaatg ccaccctgcc gggcattccg ggcgggatcg ccaccggggt
     1021 catggtgctg tacagcgacg acgaatcctt caccctgatg tgcccggaag gccatcccga
     1081 ggccggctgg aacaccttca gcgcctacat tgaaggggac acaaccgtgg ttcagatcca
     1141 gtcgttggcc cgggccaacg acccggtgtt cgagtttggc ttccgcttca tgggcggcgc
     1201 ggcccagcag gagcgtacct ggcatcacgt tctgacgggc ctggccaagc attacggcaa
     1261 ggatgctccg gtgcagatga tccgggctct ggttgacggc aagctgcagt ggtcgcaggc
     1321 caagaacgtc tggtacaact cggcgatacg cagtgtgctg aacgcccccg ttcggctggt
     1381 ccgccggctc attggccgcg gcgggcgagc cagcgcctga gctcatcacc cactcgaccc
     1441 gactctgcat cgatgcgtcg cccagaagtt gacgcggtag tgattggcgc gggaccgaac
     1501 gggctggcag ccgccatcac cctggcccag gccggccgct cggtgaccgt catcgaggct
     1561 gctgacacaa tcggcggcgg cgccaggacc gctgaactca ccctccccgg tttccatcac
     1621 gacgtgtgct ctgcgatcca cccgctcggc ctggcttccc ctttcttccg ggccgtccct
     1681 ttgcacgaac acggactcaa atggattcag cccccgggcg cgctggccca tcctctcgac
     1741 gacggaacgg cagtgctggt cagccaagat ctgcgggcga cggccggcca gttgggccag
     1801 gatggccagg cttatctcaa tctgctgggg ccgctggtcg agaactggca ggcgctgctg
     1861 gctgaaatct tgggcccatt ccggctgccc cgccggccct ttttgatggc ccgcttcggg
     1921 cggctggcaa ttcaatcggc gcggggcctg gcaactgccc gtttccgcgg cccgcgggcc
     1981 cgggccctct tcgccggttt gggtgcccat tcgatgttgc cgctcgagtc cccggtatcc
     2041 gccgctgccg gcttggtgga gggtaccttg gcgcatgcgg tgggctggcc gatggcagcc
     2101 gggggctcgc aaaggatcac ggatgctctg gttggcttgc tgcgttcgct gggcggccag
     2161 atcgtgaccg gccgccgggt caccagcctc gacgaactgc ccagcgcccg gaccgtcttt
     2221 ttcaacacat cgccaaagga gttgatccgg atcgccgggc agcgactgcc ggctggctac
     2281 cggcggaggc ttgagcgatt ccgctatggg ccgggggtct tcaaactcga ctgggcatta
     2341 gatggcccga ttccctggac cgccgaagcc tgccgccagg ccgctaccct tcatctcgga
     2401 ggcaagttgg aagagatcgc cgaggctgag gcagcggtcg cgcggggcga gcatcccgag
     2461 cgaccgttca tcattttcgc tcagcagtcg ctgttcgacc aagaccgggc tccccaggga
     2521 aagcacaccg cctggggcta ttgtcacgtt cccaacggct cgacggtcga catgaccgag
     2581 cgaatcgaag ctcagatcga acgcttcgcg cccggtttcc gcaaccggat cctggcccgg
     2641 cacagccgca attcggtcga gatggagaag tacaactcca attacgtggg gggagatatc
     2701 aatgccggga tccaggacct gcgccagatc tggacccggc cggtagctcg tctctcgccg
     2761 tacacgactc cggtggccgg cctctacctg tgctcttctt cgacgccacc cggcggcggg
     2821 gtgcatggca tgtgtggtta tcatgcggcc cgggcggcgc tgcaccgatc gctgaagtcc
     2881 ggctagccct acaacccgaa gtcaacaaag aaaccaaagc ggcccaacac aaggcgccca
     2941 gccggcggaa aagaacccgc ccatcatggc cgaggatcgg atccatctga cttcccgctc
     3001 gccatcggtt gggagcatcc gaggcttcag ggggcgtccc agccgtctgc ctgggcagcc
     3061 tgcacatatt gccgctgctc ttcgttgggg agcaagagtg gttcccgttc attcaaggcg
     3121 atggccaggt aataaacctg ggcagcctgc tcggcgtgga ggctgcggat gaatgcaact
     3181 tccagggtcc ttccgacgca gaagagaccg tgattgggcc agacgatccc gaaccgatcc
     3241 cccagggcct cgatgtttgc ggttgcgaag gcggaggtcc cactcttcat ccggggtccc
     3301 accgccaagc ctcggccggc gaaggcgtag ataaaattcc cgaggacgtt cggcaaggac
     3361 ttattcagcg cgtaaagcgc gttgatatgc ggcggctcgg tgtgagcaca ggctttgatg
     3421 cgcggatccg cccggtaggc ggccagatgt accggcaatt cgtaggaggg agggtggagt
     3481 gccttgagca cccgcccatc cagcccgact tccacgatgt ctcccggccg cattgtgctg
     3541 taggggatcc cggttggtgt gatcagaatt cggtcgctct ccggaatccg cgcactgaag
     3601 ttcccctggc tgcgcaagac caggccatag gcctcgcaac gctgggcaac ttcgaccagg
     3661 tcgtgccgta gcggccactc ttcagccaat ggtacgactc ccgggcccgg ctgccagcac
     3721 ccgagccaac tcgccggtat cttacaccgc agctgctatg cccgcctcgc cggtcagcta
     3781 ttgccgcctg gatggcgggt tgttaggatt gcctacagcc ggctcggtca ggagagcgaa
     3841 atgaagcgtt gggtgatcgg cgccgacctg ggaggcactc tgatccgcgc cattcggacc
     3901 gatcttgagg gccgaaaggt agcgcgggcc gagatccaga ctctagcgca ggaagattcc
     3961 gaggcgatcc tcggtcgaat ctttcagatg attcagcagg ccagcgaagg agcggatccc
     4021 agcgaggtgt tgggaatcgc gattggcgcg cctggcccga tcgacgagct gggacgactg
     4081 cacgatccgc ccaatttgcc gggaatgaaa gagttttccc tcggggaaca gatccaggac
     4141 cgattccaaa ttccgagcta cgccggcaac gatggcaacc tggcagcctt tggcgagctg
     4201 cgcttcgggg cgggccgagg cctggaggac ttgatctaca tgagcatcgg caccggggtc
     4261 ggcggcggga tcatttgcga cggaaagctg ctgcttggag cgcacggctt cgcagccgag
     4321 attggccacc agaccctcgt gccggacggg ccgctctgcg cttgcggcca acccggccac
     4381 ttggaggcgc tggcctccgg gccgagcatt gcccgccagg cgaaggaacg actgcaagcc
     4441 ggcgcatctt cgaccatcga ccactaccgc gaggccggca atgagttgac cgggaagtcg
     4501 gtgaccgacg ccgcccggca tggagatgaa ctggcgctcg agcttctgac ggaggctgcg
     4561 acctacatcg gcctgggact ggtgaatctg atccacatcc tggaacctca gcggatcttg
     4621 cttggcggag gggtcagcca ggccggggag ctgctcttcg aacccattcg gcagacggtg
     4681 cacgaccggg tgatgtcccc agtctttcgc ggggtcgaga tccaacccgc cgcgctcggc
     4741 aaggacgtgg ggttgatggg cgcggtcgcc tgggtactgg aacgatcgag cccggattga
     4801 cttcggcggc aagccacttg acaaccaatg gtaaaatcgt ggggtgccat gtttcattga
     4861 catttaccat tcgcgttacc cctctcgccg ggaagcctag ctccaaccgg ctttccactc
     4921 cactgtaagg attcaaccga atccgccgcg agagcctgcg cggtggaccc taagccaggg
     4981 acgaaggtcc gctttggacc tcggctcttg aggcctcctc gcaggccgaa tcgagttcgg
     5041 atcgcatgac tgctgaagta gcaccaagag atcttaggaa cactctggcc actcgccgcc
     5101 tggtagggct gtggcggctg gcggacggct tccgccagct gctgctgctc gcgaccctcg
     5161 ggatcgggct cgccgcgctg gcccgcgccg ccagctacct gctggtggga catttcgtcg
     5221 acgttgtcct gctgcgggag gacttgggcc gcgggctgcc ctgggtggcg gccggcttca
     5281 tcggcctggc gttagggcaa ggcggcctca cctacctcag tggccggctg gcggcccgct
     5341 cggcggaagg gatcacgaag cgcttgcggg agtatctgtt tgaccattta cagcggctct
     5401 cgttcgccta ccacgcccag gctcgcagcg gggacctgat tcagcgctca acctcggacg
     5461 tcgatgcgat tcgccgattc ttcgccgatc aggcgatcaa cgccggccgg atcctgctga
     5521 tgttcctggt caatctggca gtcttgattc agctggacgc ccagctggca ttgctctcgg
     5581 tggtcgtggt gccggttacc gtcacgatgt cgtacttgtt cttccgccga atctccaagg
     5641 cctacgagga ctaccaggaa caagaggccg tactctcggc cgccctccag gaaaacctgg
     5701 ccggggtgcg ggtggtgaag gcattcgccc gtcaggagtt tgagcgcagc aagttcgaca
     5761 ctgaaaacat ggagaagttc cggcgcggcc gacgcctgct catcatgcac tcgctgcact
     5821 ggccgatctc cgacattctc tccggcgcgc agaccctctt tgggctcacc ctcggcgccc
     5881 tgatggtgat ggatggccgg attagcatcg ggacctacct ggcctactcc ggcctgatca
     5941 tttggatcat ctggccgatc cgcaacctgg ggcggttggt ggtcgacatg tccagtggat
     6001 ttggttccta cggtcgggtc gcggagatca tccgcgaaga acgcgagccg ctggacgagg
     6061 gcagtttccg tccaagcggg ccgcccaggg gagagttagt tttcgagggg gtcggttttg
     6121 cgtacccgga cggcggcggt gaagtcttgg aggacatcca cttgcacgtc ctccctgggc
     6181 aggtggtcgc cctgctcggc ccgaccggtt ctggcaagac cagcctggtc aacctgctgc
     6241 cgcggttcta cgactatacc agtggcaatc tgttgctgga cggggtcgag ctgaaggact
     6301 acccgcggcg atacttgcgg cagcatgtcg gaatcgtgga acaagaaccg ttcctgttct
     6361 cgcggacgat ccgtgaaaac atcgcctacg ggatggggcg ggaggtcagc ccagccgaaa
     6421 tcgaggcagc cgcccgggcg gctgccatcc acgaagggat cctggccttt cccgacggct
     6481 acgacactct ggttggtgag cgaggggtaa ccctatccgg aggacagaag cagcgggtgg
     6541 cgattgcccg caccctgctg attgacccgc ggatcctgat tctggacgac tcgacttcct
     6601 cggtagacac ggagaccgag gccctgatcc gatcggcgct ggagcggctg atgcaaggcc
     6661 gcaccagttt catcatcgcc caccgggtcc agagtctgat gatcgccgac ctgatcttgg
     6721 tgctccaggg cgg
//
LOCUS       DATDPQ010000120        17942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3714859, whole genome shotgun sequence.
ACCESSION   DATDPQ010000120 DATDPQ010000000
VERSION     DATDPQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17942
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3714859"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VI520_02730"
     CDS             complement(<1..745)
                     /locus_tag="VI520_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HLF36830.1"
                     /translation="MSSARGRNSIVLLILGLIVIVLVFQFRSNAPQPTRLDLNQVAAE
                     IHAGRVARLVIDDNDLEIELPDKSQAISRAEPGRPAIEQLMDLGVTADDLSPERIQLE
                     IKQPSDFNQIITLVTYLLPGLLVVGLIFFMLRQAQGSNNQALSFGKSRARLFTGDQPT
                     VTFEDVAGVDEAKEELQEVVEFLKEPEKFIKLGARIPKGLLLIGAPGTGKTLLAKAVS
                     GEAGVPFFSISGSEFVEMFVGVGASRVRDL"
     gene            complement(906..1889)
                     /locus_tag="VI520_02735"
     CDS             complement(906..1889)
                     /locus_tag="VI520_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF36831.1"
                     /translation="MAEDLVGGRYQVLRPLGAGGMADVFLARDLNLQREVAVKVLRPD
                     FSRDTGFRVRFLQEARAAANLTHPNIVTIYDFGEDPQRLFIVMEFVDGTDLKTLIHRR
                     TSIPRAAALDLLIQVCDGVGYAHRAGLVHCDLKPQNILVSNAERAKITDFGIARALAS
                     IPLDEQRDIVWGSPQYFSPEQASGASPSPASDVYSLGVVLYEMLTGRLPFEAADPRLL
                     AELHQVGPIQPPSQYDPSIPPALDQIIQKVLSKEPAARYRTADQFSRVLQSFAAERPL
                     PAADVSHAATQAVRSLRTDQLDWRAVALGMLAFLAIGGLVPLWLWVWLQYR"
     gene            1972..2907
                     /locus_tag="VI520_02740"
     CDS             1972..2907
                     /locus_tag="VI520_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLF36832.1"
                     /translation="MSTGRLIYNPAAGRFPAGWLLSRAVQVLSDAGWTIDVQEVHEGG
                     EIQRVAHEAIRAGCDAVFVAGGDGTVGAVAAALSGSEVALGVLPSGTANVWAKEIGLE
                     RLDWIHLFALERAADRLARGRVHKVDMGRACGREFLLWAGIGLDAEIVNSIEPRERWA
                     KTFGTMHYGILALWNSIGWEGVELAVRADGQVWEGRYLAAIASNIRKYAGGLIELSPE
                     AKFDDGMLDFWLIGGRSLKDAVTRVVQIFLGSHSEAPGVVHFQAARALFESESELQIH
                     FDGEPYSVSSPVEFQTLRNSLNVLLPEPEGGIQQN"
     gene            2904..3386
                     /locus_tag="VI520_02745"
     CDS             2904..3386
                     /locus_tag="VI520_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36833.1"
                     /translation="MSRLRGGWLLRLAGAGIGVLIAALLLIVSRLSGLSASVAAPPPV
                     LTVIPIPISNSLPTATPQPSLIPAPLGSPTPDPQSAKQFTLGELVEVYGTQGDGLRIR
                     ESPGLEAAILVLGLESEVFQVAAGPVISDGLSWWRITSLYDPTETGWAADSFLRSFGR
                     "
     gene            3388..3714
                     /locus_tag="VI520_02750"
     CDS             3388..3714
                     /locus_tag="VI520_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HLF36834.1"
                     /translation="MELRPLKVGASRSRGLLLVDWSDGHHSEIRFANLRAACPCAQCR
                     GEPGRPALIRIPLNTAESTQLERVDSVGNYAIQLAWKDGHSFGIYTWEYLRELCPCGE
                     HTRVGS"
     gene            complement(3757..4497)
                     /locus_tag="VI520_02755"
     CDS             complement(3757..4497)
                     /locus_tag="VI520_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36835.1"
                     /translation="MSASSSPARLRRVLVVDDEPRLIHVIRLNLEHDGFEVIEATSGP
                     QALGLLRDRLPDLILLDVMMPELDGFETLQLIREISTVPVIMLTAKGEEEDRIRGLEL
                     GADDYISKPFSPREMVSRVKAVLRRTETPSSPIHEKIEVDERLSIDFDRREIFVDGAL
                     VQLRPTEFKLLFHLVQNAGWVVPHDQLLAKVWGYEYREETHYLRLYINYLRQKLEPDP
                     SNPRYILTERGVGYRFVDFRRKQPPKQA"
     gene            complement(4484..4726)
                     /locus_tag="VI520_02760"
     CDS             complement(4484..4726)
                     /locus_tag="VI520_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36836.1"
                     /translation="MPTSVLVHLTGEDPVLGEIEEVPSPSDTSVTVNNPRRRDGKDLP
                     YLDGNVVTVVWPMHRVNFIEILPTAEEEELIGFVRE"
     gene            complement(4739..5134)
                     /locus_tag="VI520_02765"
     CDS             complement(4739..5134)
                     /locus_tag="VI520_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF36837.1"
                     /translation="MLESEVAGDDGLPAIRGLAAGAIFGLRVVETGEMVSLVGRDNFT
                     LGRSGGGQAIVPDVDLDTYGAQDQGISRLHAEIRLDGEGVHVVDLDSVNGTLINGKRI
                     EPQLPRRLRHKDIIQLGRLRLQLVSRSRS"
     gene            complement(5236..6714)
                     /locus_tag="VI520_02770"
     CDS             complement(5236..6714)
                     /locus_tag="VI520_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLF36838.1"
                     /translation="MTQELDFYTLLGVPRNASVEDIRRAYREAALRLHPDKNTGPGDT
                     ELFLKVNKAYEILSDLDQRTVYDKSLVEHDVELTARSSFRAGVIQSRRKLLQLDEPQV
                     HYLLLDVQPNADLPTIRPPINLALVLDRSTSMRGARLDQLRSAVIKILQSTEHGDSTS
                     IVTFSDRAELVVSPDQARDMSVARARLSLIQASGGTEIGQGLRAGLDELEKHFSREGI
                     NHLILFTDGRTYGDEDECIRIADSCASKGITIHGVGIGNDFSDRLLDDLASRTGGSVA
                     FLDSPQAIMGVMEGIFEALGRTVASRLRLEGAVAPQVDLRSSFRLLPDPMPMGDSLPM
                     NLGNLPRDGVVRVLLELVIHPIGKMTELTLAHFTIDGDILGKGSTSVALPVQVRIPVS
                     TEPDTDPPAEQITSALSQISLYRMQEKARHEAELGQSGQAARRLENLATQLLANGEKE
                     LAKAALSEADRLTHSRRLSSEGEKVLKYGTRALLLPARAGDS"
     gene            complement(6721..7602)
                     /locus_tag="VI520_02775"
     CDS             complement(6721..7602)
                     /locus_tag="VI520_02775"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLF36839.1"
                     /translation="MTFLQRLFGKTQSAVQSPDRIETAPLDPSVIDGAALSDRILVGA
                     SQSTGVERAHNEDALFTLIGSSSGEAAVPNFGLFVVADGMGGHRSGEIASAISVRAVA
                     QRLAKDTLLRLFESDGLLDGQPLQDQVRKALQEANNSVVSRVPGGGTTLTAALILGNQ
                     VTIGHVGDSRAYAISNGQAKVLTRDHSLVKRLEELGQLTEAEAATHPQRNVLYKAIGQ
                     GANLEVDVATHPIPRNGFLLICSDGLWGVVPEAELLRIVWSAEHPQAACDELVRAANS
                     AGGPDNITVIMVYFPPA"
     gene            complement(7653..9539)
                     /locus_tag="VI520_02780"
     CDS             complement(7653..9539)
                     /locus_tag="VI520_02780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLF36840.1"
                     /translation="MKRTTKALSDTSSETAQLGQLDPGVTLQERYLVLGLQGAGGMSS
                     VYRARDLHFPNVTKAVAVKEMINMATDPTMHEMIVRNFEREADLLATLSHPAIPRIYD
                     YFTHENNSYLVMEFIEGKDLEALLRDIEGSLPEDQVVNWAIELCDVLFYLHNHKPQPV
                     IFRDVKPSNIMVDNHGSIRLIDFGIARNFQPGQKGTMIGTDGYAPPEQYRGEASPAGD
                     LYALGATLHHLLTRKDPRAEPPFSFSERPIRKINQSVSTETEAVINTALAYDPQDRFS
                     SAEAMKSALMAAAKETGILVSPRTATTAIRPRNEVQEVWSFECEDEIRGSPLVAKGMI
                     YIGCYDNNLYALNAETGEFRWKFATEGGIVGKPCSDGELVYIASEDTRLYAVSVERGA
                     LAWTYYSAGPIRSSPVISEGHIFFGSDDARLHVVNMFNGNSAWKQEVAGPIRSTPLIT
                     GDRVYFGCESSDLYCYDFRGEMKWRFGKAKRAITSSPILVEGMLYFGCRDWSLYAVEA
                     EAGWQIWKFRMGQPTISSPVFAENRIYIGSGDGRIYAVEARSGREVWHYQTEHQVNGS
                     PIIFEDSLYCGSVDNSMYCLDMRSGRLRWQFQTAGPITGTPTVANNRLYFGSHDHRVY
                     ALLT"
     gene            complement(9645..10628)
                     /locus_tag="VI520_02785"
     CDS             complement(9645..10628)
                     /locus_tag="VI520_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLF36841.1"
                     /translation="MSTPDPPVSEMQEAVRADLRSFAGQLKDDPQLYQIVEHHFGWAE
                     AAAGGKGVRPLLCLLCCAAAGGHWEAALPAATSVELIHNFSLIHDDIQDRSEFRRHRQ
                     TVWKVWGEALAINAGDALFSLARLSTQRLELEPSRLLALHRILDEACLELTKGQQRDL
                     GFSRNGEETLANYVRMIEAKTASLLAASAAAGALVAGATPSRQRAFQEFGRQLGLAFQ
                     ILDDVLGVWGAPGQTGKSVGQDLLERKPSYPALYALEHNSAFPALWEDPSSDLPSLLE
                     AMEAVGSREAASQAAGEYTNQCLSSLAAAKPLEPAAVALQGLANGLLTRRS"
     gene            complement(10625..11650)
                     /gene="fni"
                     /locus_tag="VI520_02790"
     CDS             complement(10625..11650)
                     /gene="fni"
                     /locus_tag="VI520_02790"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HLF36842.1"
                     /translation="MTKVTAQRKSDHIRVNLEENVRSGLTTGLERLRFEHNALPELDL
                     RAVDTRLTFLGRQLNVPLLISSMTGGTEQARTVNRALAEAAQQTRVAMGVGSVRAAIE
                     DPSLADTFAVREYAPDVLLFANLGAVQLNYGYGLAETQRAVELIEADALVLHLNALQE
                     ALQPEGDVNFSGLLLKIEAICRGLSVPVVAKEVGWGISPLIARRLVGAGVAAIDVAGA
                     GGTSWSQVEMHRSPDEGAAAVAAEFVDWGIPTARAVADLRQAMPELPLIASGGLRGGI
                     DLAKVLALGARIGGMAGPFLRAAVDSSETVIELINRTAKVLRVAMFATGCPDLESLRN
                     TKLVPDL"
     gene            11755..11991
                     /locus_tag="VI520_02795"
     CDS             11755..11991
                     /locus_tag="VI520_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLF36843.1"
                     /translation="MGDTYADVRTIIIDLLGAEESKITPTAKFREDLEADSLDLVELI
                     MAFEEKFGGEISDEDAQKITTVGEAVTYIDQNMG"
     gene            complement(12052..13206)
                     /locus_tag="VI520_02800"
     CDS             complement(12052..13206)
                     /locus_tag="VI520_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36844.1"
                     /translation="MSEPVSSLTQLLDRASSRRFLEAPLTEELGLAEELPFPFLAIVG
                     QPEMKLALLLGLINPNVDGVLLIGTRGTGKTTAVRSLAGLLPEVARSLCAYGCLPEDI
                     EGGGLDEVCPDCARKYGEGQSLTYRDRVQLIELPLNSGLDDVIGGIDEHSPMPRRMRL
                     RRGILARADRNLLYVDEVNLLSDEIVDSILDAAAQGSYTVRRGGTAASYRSRFVLIGS
                     MNPEEGRLRPQILDRFGLRVLVRGLQSKPLRLEGYRRAISFRGNPRQVIREYAHSTES
                     VRTELQTAREALPKVELSRQAERFGLGLIKQLGIDSLRAEITLFEAARAHAVADGRLL
                     ASPKDVRAVAPMAIRLRRSEFMKDFFRSQRKEQQELDRLLKSPTTRHNSR"
     gene            complement(13203..13676)
                     /locus_tag="VI520_02805"
     CDS             complement(13203..13676)
                     /locus_tag="VI520_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HLF36845.1"
                     /translation="MSASRVTLSQLMQPQFANLRGAVHGGWIMKLADEAGGLAAARHA
                     QKPVVTVAIDQMSFHSPIRIGDLVILEAEVTFVGNSSLETRVQVTAEDPLTAIRTHTN
                     TAYLVYVALDAAGRPTRVPRLIAETESESLRMARGERRQADRLARLKHEAQEAAP"
     gene            complement(13697..14074)
                     /locus_tag="VI520_02810"
     CDS             complement(13697..14074)
                     /locus_tag="VI520_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36846.1"
                     /translation="MASTRKPIPIYTTRGDWAALMVFPYLYNTMGEWIGWVTPEKQVY
                     NIDGIYAGWVTAEPRILRKRVLEKKLDRRSSPPAPGKIRPPATVPLPPMMAELPYEIM
                     DIFEEDIQWLTTADHGEFKQDMD"
     gene            complement(14140..14607)
                     /locus_tag="VI520_02815"
     CDS             complement(14140..14607)
                     /locus_tag="VI520_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HLF36847.1"
                     /translation="MRERARREFTRFTKFAIVGTIGAVVDFGAFNLIRGGLGIPAILA
                     QALSFSAAIASNFFWNRYWTYPDSRSKDWRQQATQFALVNLVGLAIRTPIFVLAEPWM
                     IGFAERVLPILPKLSFSLEAVALGSNLALASAVVVVLFWNFGANRVWTYSDAG"
     gene            complement(14604..15554)
                     /locus_tag="VI520_02820"
     CDS             complement(14604..15554)
                     /locus_tag="VI520_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HLF36848.1"
                     /translation="MRIVVTGGAGFIGSHLCDRLLADGHEVVAIDNLVTGQAENIAHL
                     AGHQRFSHIRHDVSNFIDVPGKVDLIWHLASPASPNPASPYGYPQLPIQTLKVGALGT
                     HNALGLARANQAIFLLTSTSEVYGDPQVHPQREDYWGHVDPVGPRSMYDEAKRFAEAI
                     TTAYHRYHGLDTRIARVFNTYGPRMRLDDGRVVPNFLCQALANQPLTIYGDGQQTRSF
                     CFVDDLIEGLCRLMLTPVHDPLNLGNPHEITVLELAEIVLRLTGSRAGVRVLADRRGP
                     ADPQQRRPDISRAKDLLEWEPRIDLEDGLQRTIENFRRRL"
     gene            complement(15583..17163)
                     /locus_tag="VI520_02825"
     CDS             complement(15583..17163)
                     /locus_tag="VI520_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HLF36849.1"
                     /translation="MRRTLTTLLFLLLTACVGPKATAGTILVSIVVDGDRLEVNLPAG
                     STVQAAVEHAGIQMGELDRVEPAVYAVLTEGAQIELWRRTERFEVESQVLPFARQTVR
                     NEGLPEGETRLLQAGSNGLEEITYRVLEEEGDLISRQVFLRSVSQEPMPEIIMVGAQA
                     AHSPLSLEGTLAYLSGGNAWLIEGNSANRRPVVVSGDLDGQVFSLSPDGRWLAFTRGE
                     VENETDFNSLWVIDLKDPAAAAIDTSARNVVHFADWSPDPDGSVLAYSTVEPRPSPPG
                     WQANNDLVLLTLSPAGRIVGRESLLPTNPGGLYGWWGTSFAWAPDGVHLAYARPDEVG
                     LIDSREPGFVPLIPLTPYLTGSDWAWVPGVAWGSNSRTLYLVDHGSPLGFEEPGSSPI
                     FDLIAVNWPGGAEISLVERAGMFSYPSASPVQILDSGEISGQVAFLQALTPLKSELSA
                     YRLMVMDRDGSNLRAIFPPEGDPGIGQAELAPAAWSPSGARLALAYRGDLWVVDVAGG
                     SGQPLTGDGLTIAFDWNP"
     gene            complement(17198..17791)
                     /locus_tag="VI520_02830"
     CDS             complement(17198..17791)
                     /locus_tag="VI520_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36850.1"
                     /translation="MSPFEANPARMPQRRLGFSALCLSLAGLACQINVGGPSPPSAPI
                     QISTLEALQLEENWRAAFEADPVAGRVTVVITERQLTAFLAARMADAQNPLLKDPQVR
                     LQAGEIQIFGVASAGPLEASALLSIQPLVDPEGVLAFNITTAEVGPVPIPDTLKQGLS
                     DLLTEAFTGKLGSLATGIRITSLAIADGEAAIVGELR"
BASE COUNT         3512 a         5406 c         5580 g         3444 t
ORIGIN      
        1 acaagtcgcg cacccggctg gccccgaccc ccacaaacat ctcgacgaat tccgatccag
       61 agatcgagaa gaagggtacg ccggcctccc cggagacggc cttggccaac aacgtctttc
      121 ccgtgccggg ggcgccgatc agcaacaatc ctttcgggat gcgagccccg agtttaatga
      181 acttctccgg ctccttgagg aattccacga cctcttggag ctcttccttc gcctcgtcga
      241 cgccggccac gtcctcgaag gtcaccgtcg gctgatcccc ggtgaacagc ctggcccggc
      301 tcttgccaaa ggagagggcc tggttgttgg atccttgcgc ctgacgcagc atgaagaaaa
      361 tcaggccgac tacaaggagc ccaggaagca gataggtcac cagagtaatg atctggttga
      421 agtcgctcgg ctgcttgatc tcgagttgaa tccgttccgg ggaaaggtca tccgcggtga
      481 cccccaagtc catcaactgc tcgatggctg ggcggccggg ctcggcccgt gagatcgctt
      541 gggacttgtc cggtaactcg atctccaaat cgttgtcatc aatcaccaag cgggcgaccc
      601 ggcccgcgtg gatctcggcg gccacctggt tgaggtccag ccgggtaggc tggggggcgt
      661 tggatcggaa ctggaagacc aacacgatca cgatcaaccc cagaatgagg agaacgatgg
      721 aattacggcc gcgtgcgctg ctcaacaact tgaagctctc ctggctggcc agcgggtgga
      781 tttacccgtc gcaggtggat tataccgtag ccaccatcca gaaaccagac ggacgcatac
      841 cgctggccgc tgcggccagt cttgccactc gcttgggccg cgaccagcaa agaatcggtc
      901 aggttctacc ggtactgcag ccagacccac aaccacaagg gcaccaatcc gccgatcgcc
      961 agaaaggcga gcatcccaag cgcgacagcc cgccagtcca gttggtcggt ccggagagac
     1021 cggacagcct gggtcgcggc gtgtgaaaca tcggccgccg gaagtggtcg ttccgcggca
     1081 aagctctgca agacccggct gaactggtcg gcggtgcgat agcgggcggc gggctccttg
     1141 gagagcactt tctggatgat ttggtcgagt gccggtggta tcgatggatc atattggctc
     1201 ggaggctgga tggggcccac ttgatggagt tcggccagca gccgcgggtc ggcggcctca
     1261 aatggcagcc ggccggtgag catttcgtac aacaccaccc ccagcgagta cacatcgctg
     1321 gcgggagaag gtgaggctcc gctcgcctgc tcgggagaga aatactgcgg gctgccccag
     1381 acgatgtccc gctgttcatc cagcggtatg ctcgccaacg cccgcgcaat cccgaagtcg
     1441 gtgatcttgg cccgctcggc attggacacc aggatgtttt ggggcttgag gtcgcaatgg
     1501 accaggcccg cccggtgcgc atagccaact ccgtcacata cctgaatgag caggtcgagg
     1561 gccgctgcac gcgggatact tgtccggcgg tggatcaggg tcttcaggtc ggttccatcc
     1621 acgaactcca tcacgatgaa gagccgttgt ggatcctctc cgaagtcgta gatggtcacg
     1681 atgttcggat gggtcaggtt ggccgcggcc cgggcctctt gaaggaagcg gacccggaag
     1741 ccggtgtccc gcgaaaaatc cggtcgcagg accttgaccg ccacttcgcg ctgcagattg
     1801 aggtcgcggg ccaggaatac gtcggccatc ccgccggcgc cgagcgggcg caggacttga
     1861 tagcggccgc caaccaagtc ttcagccatt gcagaccgat tctaaactcc gctccaaggg
     1921 gcagcaagac ctgctgggct ataatgccag gtcacctggc atgcggcgaa aatgagcacc
     1981 gggcggctaa tctacaaccc tgcggcgggc aggttccccg ctggctggct gctgagtcgg
     2041 gcggttcaag tgctcagcga cgcgggctgg accatcgacg tgcaggaagt ccatgagggt
     2101 ggcgaaattc agcgggtggc gcacgaagcg attcgggcgg gctgcgatgc agtattcgtg
     2161 gcgggcggcg atgggaccgt cggggcggtg gccgccgcgc tgagcggaag cgaggtggcc
     2221 ctgggagtgc tgccctccgg gactgctaat gtttgggcga aggagatcgg cctagagcgt
     2281 ttggactgga tccatctctt cgccctggaa agagcagccg accgactggc ccgcgggcgg
     2341 gtccacaagg tcgacatggg gagggcctgc gggcgcgagt tcctgctctg ggcgggaatt
     2401 gggctcgatg cggagatcgt gaatagcatt gaaccgcgcg agcgctgggc gaagaccttc
     2461 ggtaccatgc attatggaat cctcgctctc tggaattcga ttggttggga gggagttgag
     2521 ctggccgttc gggccgacgg acaagtgtgg gaaggtcgat atttggccgc gatcgcaagt
     2581 aatatccgga agtatgccgg cggcctgatc gagctgagcc cggaggccaa gttcgatgac
     2641 ggcatgttgg atttctggct cattggaggt cgctcgctca aagacgcggt cacccgagtc
     2701 gtacagatct tccttggctc tcactcggag gcacctggag tggttcactt tcaagcggct
     2761 cgggccctct ttgaatcgga gtcggaattg caaattcatt tcgacggtga gccttattcg
     2821 gtcagctcgc ccgttgaatt ccaaactcta cgaaattcgc tgaacgtcct cctgccggaa
     2881 ccggagggcg ggattcaaca gaattgagcc gtctgcgcgg cggatggctg ttacgcctgg
     2941 caggtgccgg gatcggagtt ctgattgcgg cgctgttgct gatagttagc cggctgagcg
     3001 gattgtcggc ttcggttgct gctccgccgc ccgtcctgac cgtcatcccc atcccgattt
     3061 cgaattcgtt gcccacagcg accccgcagc cctcgctgat tcccgctccg ctcggctccc
     3121 caactcctga ccctcagtcc gccaaacaat tcaccctagg cgaattggtg gaagtctacg
     3181 gcacccaagg agacggactt cgaatccggg agtcgcctgg cttagaggca gccattctgg
     3241 tcttgggctt ggagagcgaa gtctttcagg tagcggcggg gccggtgatc tcggacggcc
     3301 taagctggtg gcgaatcacc agtctttacg atcccacaga gaccgggtgg gcagccgatt
     3361 cgttccttag gagctttggc cgttaatatg gaacttcgtc cgctcaaagt gggggcctcg
     3421 cgctcgcggg gcttgctgct tgtagattgg agcgatggac atcacagcga aatccgtttc
     3481 gccaatttga gagccgcctg cccgtgcgcc cagtgccggg gcgaacccgg ccggccagct
     3541 ctaattcgaa tcccgctgaa tactgccgaa tcaacccagc tcgagcgggt tgattcagta
     3601 ggcaactatg ctattcaact ggcctggaaa gacgggcact cttttgggat ctacacctgg
     3661 gagtatttga gggagctctg cccctgcggt gaacacaccc gagtcggcag ttaggcgaca
     3721 acttgggtct tgcgccccgg aggcaaaacg aagcggtcag gcttgcttgg gaggttgctt
     3781 gcgccgaaaa tcgacgaacc ggtagccgac ccctctttca gtcaagatgt agcgcgggtt
     3841 ggaggggtcg ggttccagtt tctgccgcaa gtagttgatg taaaggcgaa ggtaatgtgt
     3901 ttcctctcgg tattcgtagc cccagacctt ggcgagcaat tggtcgtggg gaacgaccca
     3961 accggcgttc tgcaccaggt ggaacaagag cttgaattcg gtcggacgca actgaaccaa
     4021 agctccatcc acgaagatct cccgccgatc gaagtcgatc gaaagccgct catcgacctc
     4081 gattttctcg tggatcggtg agctcggagt ctcggttctc cgcagcaccg ctttcacccg
     4141 gctgaccatt tcccgcgggc tgaagggctt cgagatgtag tcgtcggctc ccaactccaa
     4201 gccgcgaatc cgatcttctt cctcaccctt ggcggtcaac atgatcaccg ggacggttga
     4261 aatttcccga atcagttgca gggtttcgaa accgtctagc tccggcatca tcacatccag
     4321 caagatcagg tcgggcagcc ggtcccgcag caagccgagc gcctggggtc cggaagtcgc
     4381 ttcaatcact tcaaagccat cgtgctccag attcaaccgg atgacatgaa tgaggcgggg
     4441 ctcgtcgtca acgaccagaa cccgacgcag ccgggcgggg ctactactcg cggacaaagc
     4501 cgattaattc ttcttcctcg gccgtaggca gaatctcaat gaagttgacc cgatgcatcg
     4561 gccacaccac ggttactacg ttgccatcca ggtatggaag gtccttgccg tcgcgccgcc
     4621 gcgggttatt gaccgtgacg ctggtatcgc tcggcgaggg gacttcttcg atctccccca
     4681 ggaccgggtc ttcgccagtg agatggacca agaccgaagt gggcatttcg tctcctacct
     4741 agctccgtga ccgcgagacg agctggagtc ggaggcgccc cagctgaata atgtctttgt
     4801 ggcggagacg gcgcggcagc tgcggttcga ttcgtttgcc gttgatcagc gtgccattca
     4861 ccgaatccaa atccacgacg tgtacccctt ccccgtccag ccgaatttca gcatgcagcc
     4921 tcgagatccc ttgatcctgg gccccgtaag tatccaggtc cacatcgggt acgattgcct
     4981 gcccgcctcc ggatcggcct agggtgaagt tatcccgacc gacaagcgaa accatctcgc
     5041 cggtctcaac gactctcagc ccgaaaatcg ccccggcggc cagtccacgg atcgcgggca
     5101 gcccgtcatc gccggcgacc tcggactcca gcaattcatc cagcgcgatt ggcgcagtat
     5161 gaaccaggcg agtgccacat tcactgcagt agagggttcc aacgaattcc tgatgcctgc
     5221 aagtggggca ttcgatcacg aatcaccggc gcgagcaggt aagagcaagg cgcgagtacc
     5281 atacttgaga actttctcgc cttcgcttga caggcgccga gagtgagtca agcggtcagc
     5341 ttcgctcagg gctgccttgg ccagctcctt ttctccgttg gcaagcaatt gggtcgccag
     5401 gttttctagc cggcgggccg cctgtccgga ttgcccgagc tcggcctcat gccgggcctt
     5461 ctcctgcatg cggtagagcg agatttggct gagggccgag gtaatctgct cggcaggcgg
     5521 atccgtgtcg ggctcggtgg agaccggaat gcgaacctga accggcaggg cgacgctggt
     5581 actgcctttg cccagtatat ccccatcgat tgtgaagtgg gccaaggtga gctcggtcat
     5641 tttcccaatt ggatggataa ccaactccaa caacactcgg accactccat cccgtggcaa
     5701 gttgcccaga ttcatcggca agctgtcgcc catcggcatc ggatccggga gcagacgaaa
     5761 ggatgagcgc agatcgactt gcggggcaac tgcgccctcc aaacggaggc ggctcgccac
     5821 cgtccggccg agcgcctcaa agattccctc catcacaccc atgatcgcct gcggcgaatc
     5881 caggaacgcc acgctgccac ccgtccgact ggccaggtcg tccaacagcc tgtcgctgaa
     5941 gtcgttcccg atccccaccc cgtggatggt gatccccttc gaggcgcaag agtctgcgat
     6001 ccgaatacac tcatcttcgt cgccgtaagt tcgcccatcg gtaaaaagaa tcaagtgatt
     6061 gatgccttcg cgggagaagt gcttctccaa ttcgtcgagt ccggcccgga gcccttgccc
     6121 gatctcggtt ccgccgctgg cctgaatgag gctcagccga gcgcgggcga cggacatgtc
     6181 gcgggcctgg tctggggaaa ccaccagctc ggcccgatcg ctgaacgtga caattgaagt
     6241 cgaatccccg tgctcggtgc tctgaaggat cttgatcacc gccgagcgca gctggtcaag
     6301 ccgggcaccc ctcatcgagg tcgagcggtc aagcaccagt gccaggttga tcgggggtcg
     6361 gatggttggc aaatcggcgt tcggttggac atctagcaac agatagtgga cctggggctc
     6421 gtcgagttgg agcagtttgc gacggctctg gattactccg gccctaaaac tagaccgggc
     6481 agtcagctcg acatcatgtt cgaccagact cttgtcatag acagtccgct gatccagatc
     6541 gctgaggatc tcataggcct tgttgacctt caagaaaagt tcagtgtctc cggggccggt
     6601 gttcttatcc gggtgcaggc gcagggcagc ctctcggtac gctcggcgaa tatcttccac
     6661 cgaggcgttt cgagggaccc caagcaacgt gtagaaatcg agttcttgag tcacgagatc
     6721 tcaggctggt ggaaagtaga ccatgattac agtgatattg tccggcccgc cggcagaatt
     6781 agccgcccgg accagttcat cgcaggccgc ctgcgggtgc tcggcactcc agacgatgcg
     6841 aaggagttcg gcttcgggga cgacacccca cagaccgtcc gagcaaatca gcagaaaacc
     6901 gttgcgcgga atcgggtgag tagcgacatc cacctctagg tttgcgccct gcccgatcgc
     6961 cttgtaaagc acgttgcgct gcgggtgggt tgcagcctcg gcctcggtca actgaccgag
     7021 ctcctcgaga cgcttgacga gtgaatgatc tcgagtgagg acctttgcct ggccgttgct
     7081 gatggcatag gctcggctgt ctccaacgtg cccgatggtc acctggttgc cgaggatcaa
     7141 ggccgcggtc aaagtcgtgc cgccacctgg tacccgactg accaccgaat tgttggcttc
     7201 ttgaagcgcc ttgcgtacct ggtcctgcag tggttggccg tccagcagac cgtcggactc
     7261 gaagagccgc agcagagtat ccttggccag gcgctgagcg acggctcgaa cggagatggc
     7321 gctggcgatc tcacccgagc ggtgtccacc catcccgtcg gcaaccacaa agagtccgaa
     7381 gttcggaacg gctgcctcac cggaagaact gccaatcaat gtgaacaggg catcttcgtt
     7441 gtgggcccgc tcgactccgg tagactggga agctccgacc aggatcctgt cactcagggc
     7501 cgcgccatcg atgaccgaag gatccaaggg cgcagtctcg atccggtccg gagactggac
     7561 cgcgctctgg gtctttccga agagtcgctg gaggaaggtc atgctggcag cagctggcca
     7621 agcctgatct tggcaggcaa ctggctcagt tcctaggtga ggagggcata gacccggtgg
     7681 tcgtgagagc cgaagtacaa ccggttgttg gcgaccgtgg gtgtcccggt gatcggtccg
     7741 gcggtctgga actgccagcg cagtctcccc gaccgcatat ccagacagta catcgagttg
     7801 tccaccgacc cgcagtacag ggagtcctca aagattatcg gcgagccatt gacctgatgc
     7861 tcggtctggt aatgccaaac ctcgcggccg ctgcgggcct cgaccgcata tatccttcca
     7921 tcaccgcttc cgatgtagat ccggttctcg gcaaacaccg gtgaggagat cgtcggctgg
     7981 cccattctga acttccagat ctgccagcct gcttctgctt ccaccgcgta caatgaccaa
     8041 tccctgcaac cgaaatacaa cattccttca accaagatcg gtgaggaggt gatggctcgt
     8101 ttcgctttgc cgaaccgcca cttcatctcg ccgcggaaat cgtagcagta gagatcgctg
     8161 ctctcacatc caaaatacac tcggtctccc gtgatcaggg gagtcgaccg aatcggaccg
     8221 gcgacttcct gcttccaggc tgagttgcca ttaaacatgt tgacgacgtg gagccgggca
     8281 tcatcgctcc cgaagaatat gtgtccctcg gatatgaccg gtgaggagcg aatagggccg
     8341 gccgaataat acgtccaggc cagagcgcct cgctcgacgg aaaccgcata cagccgcgta
     8401 tcttccgaag cgatatacac cagttcgccg tctgagcagg gcttaccgac aattccgcct
     8461 tcggtcgcaa atttccagcg aaactctcca gtttcagcat tcaaggcata caggttgttg
     8521 tcgtagcagc cgatgtaaat catgcccttc gcaaccagcg gggagccgcg gatttcgtcc
     8581 tcgcactcga aagaccaaac ctcctgaact tcgttgcgtg gacggattgc ggtggtggcg
     8641 gtccgcggag agaccaggat tccagtctct tttgcggcgg ccatcaatgc cgacttcatc
     8701 gcttctgcgg aagagaagcg atcctgtggg tcgtaggcca gggctgtgtt gatgacggcc
     8761 tcggtttcgg tcgacacact ctgattgatc ttgcgaatcg gacgctcaga aaacgagaag
     8821 ggtggttcgg cgcggggatc cttgcgggtg agcagatggt gtaaggtcgc ccccaaggca
     8881 taaagatcgc cggcgggaga agcctcacct cgatactgct ccggcggggc atacccgtcg
     8941 gtaccgatca tggtcccttt ctgccccggc tggaaattcc tggcgatgcc gaagtcaatc
     9001 agccggatgc tgccatgatt gtccaccatg atattggagg gcttgacgtc tcggaaaatc
     9061 accggctggg gcttgtggtt atgcaaatag aacaggacgt cgcagagctc gatagcccag
     9121 ttaaccacct gatcctcggg cagcgaaccc tctatgtcgc gcagcagcgc ctccaggtcc
     9181 ttgccctcga tgaattccat gaccaaatac gagttgtttt cgtgggtgaa gtaatcgtag
     9241 atccgaggaa tcgcgggatg ggaaagggtg gccagcaaat ccgcttcccg ctcgaaattt
     9301 cgcacgatca tctcgtgcat cgtcggatcg gtggccatgt taatcatttc cttgaccgcc
     9361 accgccttgg tcacgttggg gaaatgcagg tcgcgggcgc ggtagaccga actcatgcct
     9421 cccgcgccct ggaggccaag caccaggtac cgctcttgca gcgtgacgcc gggatcaagc
     9481 tggccaagct gggcggtctc gctggaggtg tccgaaaggg ccttggtggt cctcttcata
     9541 ttataagaat gctccttctg gggtccaaag gagcgctcgc cggagattat agcatgcgcc
     9601 ctttggcacg caatgaaacc gtcgccctat gggaggcttg gggctcagga gcgccgggtt
     9661 aggagcccat tggccaggcc ctgcagggcg actgccgcag gctctagcgg cttggccgct
     9721 gcgaggctgc tcaagcactg attagtgtac tcgcctgcgg cttgagaggc cgcttcccga
     9781 gaacccacag cctccatggc ttctagcagt gagggcaggt cgctgctggg atcttcccat
     9841 agcgccggga aggcggagtt gtgttccagc gcatacaggg ccgggtagga aggtttgcgc
     9901 tccaacaggt cctggccgac cgacttgccg gtctggcctg gagcgcccca gacgccaagg
     9961 acatcatcca gaatctggaa tgccagtccc agctgccgcc cgaactcttg gaatgcccgt
    10021 tgacgggatg gggttgctcc tgccaccaag gcgcctgccg cggcactggc ggccagcagg
    10081 gaggcggttt ttgcctcgat cattctcacg taattggcga gggtttcctc gccgttccgg
    10141 ctgaagccga gatcgcgctg ctgccccttt gtgagctcga ggcaagcttc atccaggatt
    10201 cggtgcaggg cgagcaggcg ggagggctcc agttcgagcc gttgggtcga caagcgggcg
    10261 agcgagaaca gtgcgtcgcc ggcgttgatc gccagggcct cgccccacac cttccagacc
    10321 gtttgccgat gccgccggaa ctcacttcgg tcctggatat catcatgaat gagtgagaaa
    10381 ttgtggatca gctcaaccga ggtggcggcg gggagggcgg cttcccagtg tcccccggca
    10441 gcggcgcagc agaggaggca gagcaaggga cggaccccct tcccacccgc ggcagcctcg
    10501 gcccatccga agtggtgctc gacgatctga taaagctgcg ggtcgtcctt cagctggccg
    10561 gcgaagcttc gcaggtcggc ccgcacggcc tcctgcattt cgctgaccgg cggatctggc
    10621 gtgctcaaag gtccgggacc agctttgtgt tgcgcaggga ttccaggtcc ggacagccgg
    10681 tcgcgaacat agcaactcgc aacaccttgg ccgttcgatt gatcagctcg atcaccgttt
    10741 cgctggaatc gacggcagcc cgcaagaacg gaccggccat ccctccgatc cttgccccta
    10801 gggccaggac cttcgccagg tcgatccccc cgcgcaagcc gccggaggcg atcagcggga
    10861 gctcgggcat cgcctgccga agatcggcca ccgctcgcgc agtggggatt ccccagtcga
    10921 caaactcggc cgccacggcg gccgccccct cgtctgggga gcggtgcatc tctacctgag
    10981 accaggacgt tccgccggca ccagccacgt cgatcgcggc cacaccggcg cccaccaacc
    11041 gacgggcgat taaaggagag atcccccagc ccacttcctt cgccaccacc ggcactgaga
    11101 gacctcggca aatcgcttcg atcttgagca gcagaccgct gaaattgaca tcgccttcgg
    11161 gctgcagggc ctcctgcagg gcgttgagat gcagcacgag cgcgtcggcc tcgatcagct
    11221 cgaccgcccg ctgagtttcg gccagcccat acccatagtt cagctgcacc gctcccagat
    11281 tggcaaacaa cagcacatcg ggtgcgtact cccgaacggc gaaggtgtcg gccagactcg
    11341 gatcttcgat cgcagcccgc accgagccca ctcccatcgc tacccgcgtt tgctgggcgg
    11401 cctcggccag ggcccgattg acggtccgag cctgctcggt gcctccggtc atcgaggaaa
    11461 tcaggagagg gacattgagt tgccggccga gaaacgtgag ccgggtatcg accgcgcgca
    11521 gatcgagctc tgggagggcg ttatgttcga accgcagtcg ctccagcccg gtggtcagac
    11581 cggacctcac attctcttca aggtttaccc gaatgtggtc cgatttgcgc tgggccgtaa
    11641 ctttggtcat ttcggggtgt tgaatgggga gatcatatca gcggggtagg gcggcgtcaa
    11701 acttggagag ttgaattaca ataccgccgc aaacttgggc gctggaggga tgccatgggt
    11761 gatacttatg ccgacgtgcg aaccatcatc atagacctgc tgggtgcgga ggaatcgaaa
    11821 atcacaccaa ctgcgaaatt ccgcgaggat ctggaagcag actcgctcga tctggtcgag
    11881 ctgatcatgg catttgaaga gaagtttggc ggagagatct ctgacgagga cgctcagaag
    11941 atcacgaccg ttggtgaggc tgtgacctac atcgatcaga acatgggtta ggaaccgctg
    12001 atcaggtgtg ctaactggcc agccatcgct ggccagtttg cttgtcgccc tctacctgga
    12061 gttatgccga gtggtcgggg acttcagcag gcggtcgagt tcttgttgtt ccttgcgctg
    12121 cgaacggaag aaatccttca tgaactccga gcgccgcagg cggatcgcca tgggagccac
    12181 ggctcgcaca tccttggggg aggccagcag tcggccgtcg gcaaccgcgt gagcccgggc
    12241 ggcttcgaaa agggtgatct ccgcccgcag cgagtcaatc cccagctgtt tgatcaggcc
    12301 aagcccaaac cgttctgcct ggcgtgagag ctccactttg ggtagcgcct cccgggcggt
    12361 ttgcagctcg gtccgaactg actcggtgga atgggcgtat tcgcgaatta cttggcgcgg
    12421 attaccgcga aaggaaatgg ccctccgata gccctccaac cgcagcggct tgctctgcag
    12481 gccgcgcacc aacacccgta gcccgaatcg gtcgaggatc tgggggcgca gacggccctc
    12541 ctccgggttc attgagccga tcaacacgaa gcgagagcga taactggccg cggtcccgcc
    12601 tctccgtacg gtatacgagc cttgggccgc cgcgtccagg atcgagtcga cgatctcgtc
    12661 agagagaagg ttcacctcat cgacgtagag caggttccga tcggcccggg ccagaatccc
    12721 tctcctcaat cgcattcggc ggggcatggg tgagtgctca tcgatcccac cgatcacgtc
    12781 gtccagaccg gagttcaggg gtagttcgat gagttgcact cggtcgcggt aggtgagcga
    12841 ctggccctcc ccatacttgc gggcgcaatc cgggcagacc tcgtccaagc cgccaccctc
    12901 gatgtcctcg ggcaggcagc cgtaggcgca gagactccga gccacttcgg gaagcaggcc
    12961 ggcgagactg cggactgcgg tggtcttgcc cgtgcctcga gttccgatca gcagcacacc
    13021 atcaacattc gggttgatga gtcccaacag cagcgcaagt ttcatctcag gctggcccac
    13081 gatcgccaag aaggggaacg gcagctcctc ggccaaccca agttcctcag tgaggggggc
    13141 ctccaggaac cgacgcgagc tcgcccgatc caatagttga gtcaggctgc taactggctc
    13201 gctcacggag cagcctcctg cgcttcgtgt ttcaaacgcg cgagtcgatc cgcctggcgg
    13261 cgttcgcccc gcgccatgcg cagcgactcg gactcggtct ccgcgatcag cctgggcacc
    13321 cgggtcggcc ggccggctgc atcgagggcg acatagacca ggtaggcggt gttggtgtgg
    13381 gtgcgaatag cggtcagggg gtcttctgcc gtgacctgta cccgggtctc cagcgatgag
    13441 ttcccaacga aggtgacctc tgcttccagg atgaccaggt caccaatccg gatgggtgaa
    13501 tggaagctca tctggtcgat cgcgacggtg acgacgggtt tctgggcgtg tcgggcggcg
    13561 gcgaggccgc cagcttcgtc ggcgagtttc atgatccagc cgccatggac cgctccccgc
    13621 agattggcga attgaggttg catgagctgg ctgagggtga cccgggaggc gctcaccggt
    13681 ttggaggcgc tgggggtcaa tccatgtcct gcttgaattc gccatgatcg gcggtcgtga
    13741 gccactggat atcctcctcg aagatatcca tgatttcgta gggaagctcg gccatcatcg
    13801 gcgggagagg gacagtagcc ggaggtcgga tcttacccgg cgctgggggg gagctgcgcc
    13861 gatccaactt cttttcaagg acccgcttgc gcaggatgcg aggttcggcg gtgacccagc
    13921 ccgcgtagat gccatcgatg ttgtagacct gcttttcggg agtcacccag cctatccatt
    13981 cgcccatggt gttgtagagg taggggaata ccatcaaggc ggcccaatcg ccgcgagtgg
    14041 tgtaaatcgg aatgggtttg cgggtggagg ccaatggact acttaactac tgattataca
    14101 agccgggggc gccggcactg attacgacca gctttcagct cagccagcgt ccgagtaggt
    14161 ccatactcga ttcgctccga agttccagaa caagacgaca acaaccgcgc ttgccagcgc
    14221 cagattggat cccagggcaa ccgcttcaag tgaaaagcta agcttcggga ggattggcag
    14281 cacccgttct gcgaagccga tcatccaggg ttcggccagc acgaagatcg gggtgcgtat
    14341 agccaagcca accaagttta cgagcgcgaa ctgagtcgct tgctggcgcc aatccttgct
    14401 gcgagagtcg ggataagtcc aatatcgatt ccagaagaag ttgctggcga tcgccgcgct
    14461 gaaggataag gcctgggcga ggatcgctgg gatcccgagg ccgccgcgga tcagattgaa
    14521 tgcgccgaaa tcgacgaccg ccccgatagt gccgacgatc gcgaacttcg tgaaccgggt
    14581 gaattcgcgt cgggcacgct ccctcacaat cgcctccgga aattctcgat ggtccgctgc
    14641 aacccgtcct ccaaatcgat ccgcggctcc cattcgagca ggtccttggc acggctgatg
    14701 tccggcctgc gctgttgcgg atcggcgggg cctcgccgat ccgcaagcac ccggacgccc
    14761 gcccggctgc ccgtcagcct aaggactatc tctgccaatt cgagtacggt gatttcatgc
    14821 gggttgccta gattcagtgg gtcgtgaacc ggggtaagca ttaggcggca gagtccttcg
    14881 atcagatcgt cgacaaagca gaagctccgg gtctgttgtc catcgccata gatcgtgagc
    14941 ggctggtttg cgagagcctg gcagaggaaa ttcggcacta ctcgtccgtc atctaggcgc
    15001 attcggggtc cgtaggtgtt gaacactcgg gcgattcggg tgtcgagtcc gtggtaccgg
    15061 tggtaggcag tagtgatcgc ttcggcgaat cgtttggctt cgtcgtacat cgagcgcgga
    15121 cccaccggat caacgtgccc ccagtagtcc tcgcgctgtg gatgcacctg cggatcgccg
    15181 tagacctcgg atgtggaggt gagcagaaat attgcctgat tggcacgtgc aagcccgagg
    15241 gcattgtggg tgcccagggc cccgaccttg agggtttgga ttgggagttg aggataccca
    15301 taaggcgagg cgggattggg gctggcaggc gaggccagat gccagatcag atcgaccttg
    15361 ccgggcacgt cgatgaagtt tgaaacgtcg tgtcgaatgt ggctaaagcg ttggtggccg
    15421 gcaagatggg cgatgttctc ggcctgccca gtcaccaggt tgtcgatagc gacgacttcg
    15481 tgcccatcgg ccagcaggcg gtcgcacaga tgggatccaa tgaaaccggc acccccggtt
    15541 acgacgattc gcacccgatt tggtcctctg cccccgcttc cgtcacgggt tccaatcgaa
    15601 ggcgatggtc agcccatcgc cggtgagagg ctgaccgctg ccgccggcca catcgacaac
    15661 ccagagatca ccgcgataag ccagggctaa cctggctccg cttggcgacc aagcggcggg
    15721 ggccagttcc gcttgaccaa tcccagggtc accttccggg gggaagatcg ctcgaaggtt
    15781 gctgccgtcg cggtccatga ccatcaaacg gtatgcactg agttcgctct tgagcggggt
    15841 cagggcctgg aggaaggcga cctggccgga aatctctcca ctatcaagga tctggacagg
    15901 cgaggccgag ggataggaaa acatcccggc gcgctcaacc agcgagattt ccgcgccgcc
    15961 gggccagttc actgcgatca gatcaaagat cggcgaagac ccaggctcct cgaagcccag
    16021 cggagatccg tgatccacca gatagagggt gcggctgttg gacccccagg ctacgcccgg
    16081 cacccaggcc caatcgctgc cggtcaggta cggagtgagc ggaatgagcg gcacgaagcc
    16141 cggctcgcgc gagtcgatca agcccacttc gtccggccgg gcataggcga ggtgaacgcc
    16201 gtccggagcc caggcgaatg aggttcccca ccatccataa aggcctcccg gattggtggg
    16261 gagcagcgat tcccttccca cgatccgccc agcggggctc agggtcaaca gcaccaggtc
    16321 attattggcc tgccagcccg ggggcgaggg acgcggttcg acagtcgaat aggcaagaac
    16381 cgaaccgtct gggtcggggg accagtcggc aaagtgcacc acgttgcgcg cactcgtgtc
    16441 gatggctgcc gcggccggat ccttaaggtc gatcacccag agggaattga agtcagtttc
    16501 gttctccacc tcaccccggg tgaacgccag ccagcggcca tccggggaaa gactgaagac
    16561 ctggccatcc agatcccccg acacgaccac cggccgccga ttggcggaat taccttcaat
    16621 gagccaagcg ttgccgccgg acaggtaggc taaggtcccc tcgaggctga gcggggagtg
    16681 agcggcttgg gctccaacca tgatgatctc gggcatcggt tcctgggaaa cgctgcgcag
    16741 gaaaacctgg cgtgaaatca ggtcgccctc ttcttcgagc acccgatagg tgatctcctc
    16801 aagcccattt gagccggcct gcaacagccg ggtctccccc tcgggcagcc cctcgttgcg
    16861 gacggtctgc cgagcgaacg gcagaacttg cgattccact tcgaatcgtt cggtccgccg
    16921 ccacagctca atctgggcgc cttctgtgag cacggcatag actgccggct caacccggtc
    16981 gagctcaccc atctgaattc cggcatgttc gaccgcggcc tgcaccgtgg atccggccgg
    17041 aaggttgact tccagccgat caccgtctac cacgatgctg accaggatcg tcccagcggt
    17101 agccttagga ccgacgcagg cagtgagcag aaggaacagg agtgtggtga gagtccgtct
    17161 cattgtggag cggatgtccc gttggtataa tcgcccgcta ccgaagctcg ccgacaatcg
    17221 cggcttcccc atcggcaatg gcgagcgatg tgattcggat ccccgtggcc agcgaaccga
    17281 gtttgccagt gaaagcctcg gtaagcaaat ccgagagacc ttgtttcagc gtgtcaggga
    17341 tcggaactgg accgacttcg gcggtcgtga tattgaaggc caggacaccc tcgggatcaa
    17401 ccagaggttg aatactcaac agggcgctcg cctctagtgg tccggcgctt gcgactccaa
    17461 agatttggat ctctcccgcc tggagccgga cctgcggatc ttttaggagc gggttctggg
    17521 catccgccat tcgggccgca aggaatgcgg tcagctgtcg ttcggtgatg accaccgtca
    17581 cccggccggc aaccggatcg gcctcgaatg ccgcccgcca gttctcttcg agctgcagcg
    17641 cttccagagt cgagatctgg ataggtgctg aaggcgggct cggacctccg acattgatct
    17701 ggcaggccaa gccggccaag ctcagacaga gcgccgaaaa accaaggcgc cgctggggca
    17761 tgcgagcagg atttgcttcg aaggggctca taggcttgaa aaacggggcg ggattatagc
    17821 atgcagggaa attcggagcc tcttcggcaa cggctggagg cagtactcct agccgccccc
    17881 ggccccgcca gccttgaaca gttggcggcc gcactgcagg tcgcgccggc cctggtggac
    17941 ca
//
LOCUS       DATDPQ010000121         3677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1360166, whole genome shotgun sequence.
ACCESSION   DATDPQ010000121 DATDPQ010000000
VERSION     DATDPQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360166"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VI520_02835"
     CDS             complement(<1..238)
                     /locus_tag="VI520_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36851.1"
                     /translation="MEIRRAISSDIPRLMAMDPGYQTDHVWQMAVSAHSPDTSVIFRE
                     VRLPRPMRVEYPRDPRRLADDWTHASEILLAESEH"
     gene            complement(259..921)
                     /locus_tag="VI520_02840"
     CDS             complement(259..921)
                     /locus_tag="VI520_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36852.1"
                     /translation="MEEALLLIASLQGWIYTLLLLTSLVYLRLAMQSNAEVRKSLFGL
                     ERERAASRRTRALAMLGLIGLAGLATFFLSNFLVPSVPAVTRPTLVPTISLLTTAGEA
                     LGSAVGPQTPDSSGCSNPGATISSPSSGAQVQGLVEVRGSANIPNFAFYKIEIKASDS
                     SANWQVVTAGTEPKIEDVLGVWDTSLVINGVYLLQLVVTDTAGNAPLPCALEVVVLPT
                     GG"
     gene            complement(927..1745)
                     /locus_tag="VI520_02845"
     CDS             complement(927..1745)
                     /locus_tag="VI520_02845"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HLF36853.1"
                     /translation="MEEIDLLANRIRESTDLRPKVGMILGSGLGGLADLLESSVSLLT
                     GELPGWPTSTVEGHAGRLVIGVLEGMPVCVLQGRVHYYEGYSMARIGLPIRVLQRLGA
                     DMLIVTNAAGGVNPAFEPGDVMLINDHINLIGMAGLSPLRGPNLQEFGPRFPDMSQAY
                     DRQLQALARQVAAQEGLVLREGVYICLGGPAFETPADLRFLRTIGVDAVGMSTVPEIV
                     VARHGGMRALGFSGISNKVSLDGEKLTTHQEVLEAGRRVGPKLERLIRGVLRRL"
     gene            complement(1766..2614)
                     /locus_tag="VI520_02850"
     CDS             complement(1766..2614)
                     /locus_tag="VI520_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLF36854.1"
                     /translation="MKPAIDRFLSHLRVEQERAKNTVSAYSIDVRQFEQVLSARAGGA
                     VKPHDLSTRMVSEYSNWLVEQGYRPSTVSRKMAAARAFLLYLAEKERTVNRRIPDQLQ
                     PPSTPRRLPRTLSEDEIRRLLEAPGRVESPRALRDSAVLALLYSAGMRASEAVQVNLE
                     DLDLKAGTLVRRGPEPVILQLAGSVGALGRYLTAGRPHLVRDARERALYLNLRGKRLS
                     RQGLWLVVKRWAAAADLGADLSPHSLRHSLIRHLLQQGKSKRDIQAMLGLSSPNAIRV
                     RLTAKR"
     gene            complement(2693..>3677)
                     /locus_tag="VI520_02855"
     CDS             complement(2693..>3677)
                     /locus_tag="VI520_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLF36855.1"
                     /translation="MEYRKLGKTGLTVTPLCLGTMQFGWTADEETSIKILTRAAERGI
                     NFIDTADVYSRWVAGNPGGVAEGIIGKWLGMGIVRRDQIVLATKVRGRMGEGPNEQGL
                     SRLHITEAVKNSLHRLGTDYIDLYQTHSVDPETPIDETLRALDDLVHQGVVRYLGCSN
                     YPAWRLMQALWSSDRMGLEGFVCLQPEYSLLARQQYEQELEQVCQTYGLGVIPYSPLA
                     RGFLTGKYRKDEPPPADGRLAGSPRLEEYAREKQVWQVLASLKELGRKHAASLSQLAL
                     AWLLQRPTVTSPIIGPRSLEQLDDNLGALEVLLEPQEVAELDQVSAWMDPS"
BASE COUNT          707 a         1171 c         1114 g          685 t
ORIGIN      
        1 catgctcaga ctcggccaac aagatttcag aggcatgcgt ccaatcatca gcgagccgtc
       61 ggggatcccg gggatactcg acccgcatgg gacggggcag cctgacctcc cggaatatta
      121 cgctggtgtc cggcgagtgg gcgctcaccg ccatttgcca gacatggtcg gtctgatagc
      181 cgggatccat agccattaac ctcggaatgt cgcttgaaat cgcccgacga atttccatcg
      241 aagcttggcc tcaggtcctc agcctccggt gggcagcacg acgacttcca gagcacacgg
      301 taagggagcg tttccggcag tgtcggtgac gaccaactgt agcaggtaca ccccgttgat
      361 tacaaggctg gtgtcccaga cccccagcac atcctcgatc ttgggttcgg taccggcggt
      421 gactacctgc caattggcgg acgaatcgct ggccttgatt tcaatcttgt agaaggcgaa
      481 attggggata ttggcgctgc cccggacctc gaccagtcct tgaacttgcg ctccggagga
      541 aggtgagctg atggtcgcac ccgggttcga gcagccgctg ctgtctggag tttgtggccc
      601 gactgcgcta ccgagtgctt cgccggccgt cgtgagcagt gagatggttg gcacgagagt
      661 gggcctggtg acggccggga ccgaaggaac caggaagttc gacaggaaga aagtagccag
      721 gccggccagc ccgatcagcc cgagcattgc cagcgcccga gtgcggcggg acgcagcccg
      781 ctcccgctcc agtccgaaga gtgacttgcg gacctcggca ttcgattgca tggctagcct
      841 gagataaacc aacgaagtca gcagcaggag cgtatagatc catccctgca gggaggcgat
      901 gagcagcaga gcctcttcca tctcggctac agcctgcgca gcacaccgcg aatgagccgc
      961 tcgagtttcg gtcctacccg ccggcccgcc tccagcactt cttgatgggt ggtcagcttc
     1021 tcaccgtcca gacttacttt gttgctgatc ccggagaatc ccaaggcacg cattccgccg
     1081 tgccgggcga ctacgatctc aggcaccgtg gacatcccga ccgcgtccac cccgatcgtc
     1141 cgcaggaacc gtaaatcggc gggagtctcg aaggctggac cgcccaggca gatatagacg
     1201 ccttcccgca gcacgagacc ctcttgggca gcgacctgac gagccagcgc ttggagttga
     1261 cgatcgtagg cttggctcat gtctgggaaa cggggcccga attcctgcaa gttcggaccg
     1321 cgcaggggag agagcccggc catcccgatc aggttgatgt gatcgttgat caacatgaca
     1381 tcgcccggtt caaacgccgg attgactccc ccggccgcgt tggtcacgat cagcatgtcg
     1441 gcgccaaggc gctggagcac ccgaattggc agcccgatcc tggccatcga gtacccctcg
     1501 tagtagtgca cccgaccttg caagacgcaa acgggcatgc cttcgagcac cccgatgacc
     1561 aagcgacccg catggccttc aaccgtcgat gttggccagc ccggcaactc cccggttagc
     1621 agcgacactg aagattcgag caaatcggcc aagccgccca acccggagcc caggatcatt
     1681 ccgaccttcg gacgcagatc cgtggattcc cggattcgat tcgccagcaa gtcgatttct
     1741 tccatgctcg ggaattgatc gtaattcatc gcttggcggt caaccgcacc cgaattgcgt
     1801 tgggactgct caagccaagc atggcctgga tatcgcgctt gctcttccct tgctgcagca
     1861 aatgtcggat caacgagtgg cgcaacgagt gcggggagag atcggcgccc aggtctgccg
     1921 cggcggccca gcgcttgacg accagccaga gcccttgtcg gctcaagcgc ttgccacgca
     1981 gattcaggta cagggcccgc tccctggcgt cgcgaacgag atgcggtcgg ccggcggtga
     2041 ggtaacgccc caaggctccc accgatccgg cgagctgcag gataaccggc tccggtcccc
     2101 gccgtaccaa agtcccggcc ttcaagtcaa gatcctcaag gttgacctga accgcctccg
     2161 aggcccgcat gccggccgag tacaacaagg caagcacagc tgagtcacgc aaggcgcggg
     2221 gactctcgac ccggccaggg gcttccagca gccgccgaat ctcgtcctcc gatagagttc
     2281 gcggcaatcg acggggagtg cttggaggtt ggagttggtc cggaatccgg cggttgacgg
     2341 tccgctcttt ctctgccagg tacagcaaga aggctcgagc ggcggccatt ttccgcgaga
     2401 ccgtggaggg ccggtaaccc tgttcaacga gccagttgga atactcgctg accatgcggg
     2461 tgctcaggtc gtgcggtttg acggccccgc cggcccgtgc gctcagcact tgctcaaact
     2521 ggcgcacgtc aatcgaatag gccgagacgg tattcttagc ccgctcctgc tccactcgga
     2581 gatggctgag aaaacgatca attgccggct tcatctggct gctgatgctc gccctgggca
     2641 attatacatc tcgccgggct acttgtcata agtgcgcaag ggagaggccc ggttagctgg
     2701 ggtccatcca ggcgctgacc tgatcgagct ctgcgacctc ttgcggctcc agcagaacct
     2761 cgagagcccc aagattgtcg tccagctgct cgagcgaacg ggggccgatg atcgggcttg
     2821 tgaccgtcgg tcgctgcagc aaccaggcta gggccagttg ggaaagcgag gcggcgtgct
     2881 tgcgccccaa ctctttgagg ctggcaagca cttgccagac ttgcttctca cgggcgtatt
     2941 cttcgagccg cggactcccg gccaggcggc cgtcggccgg tggcggctcg tctttccggt
     3001 actttccagt gagaaagccg cgggccagcg gactataagg gatcaccccc agcccgtacg
     3061 tctggcatac ctgctcgagc tcctgctcgt actgctgccg ggcaagcagg ctgtactcgg
     3121 gctgcaggca cacaaagccc tccaggccca tccgatccga gctccagagc gcctgcatga
     3181 gtcgccaagc tggataattc gagcatccga ggtagcggac cacaccctgg tggacaaggt
     3241 cgtcgagcgc ccgcagggtc tcatcgatgg gcgtctcagg atccaccgaa tgggtctggt
     3301 acaggtcgat atagtcggtc ccgaggcggt gcagggagtt tttcactgcc tctgtgatat
     3361 gcaaccgcga gaggccctgc tcgttcggcc cctcccccat gcgaccccga accttagtcg
     3421 ccagcacgat ctggtcccgc cggacgattc ccatccccaa ccacttgccg atgatgccct
     3481 ccgcaacccc acccggattg ccggcgaccc agcgggagta aacgtcggcg gtatcgatga
     3541 agttgattcc gcgctcggcg gcccgggtga gaatcttgat tgaagtttct tcatcggctg
     3601 tccagccgaa ttgcatggtg cccaggcaca gcggggtgac tgttagcccg gtctttccta
     3661 gcttccggta ttccata
//
LOCUS       DATDPQ010000122        19131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_828570, whole genome shotgun sequence.
ACCESSION   DATDPQ010000122 DATDPQ010000000
VERSION     DATDPQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828570"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VI520_02860"
     CDS             complement(<1..180)
                     /locus_tag="VI520_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36856.1"
                     /translation="MAGIASTIRSWKAYSWWSRLLTLQAYPLLMYLIAIRVWAEILPG
                     TWGWVTALSVAYVLAL"
     gene            complement(585..1154)
                     /locus_tag="VI520_02865"
     CDS             complement(585..1154)
                     /locus_tag="VI520_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36857.1"
                     /translation="MFPTLVFAAGVFGMLPGLIPPFSISALWVGIGLAALAIPVGLWN
                     WWTYNWWARLSILLLWTLQILLLAARGWSHLIPIVWIWLVPILGAYVLAWLLPALNPV
                     LSGVLWREQWTPQTRMGRAIMGVVLAVGPSAGVLGASFGMYGSRFGEANLVYLALATL
                     GSIAAIGIAFANAYVLWPERPWSKQAGPS"
     gene            complement(1373..1993)
                     /locus_tag="VI520_02870"
     CDS             complement(1373..1993)
                     /locus_tag="VI520_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36858.1"
                     /translation="MSKRSKGHPKVIWAWWKVLSLAILVFGVFGALPGFLPPVSISAM
                     RLGWALAIPAALAALWSWWNFSWWARLAGAALWSLQILVTGARAWTLLIGITWIWLVP
                     IVSAYLLAWAMPAMQPLVSETLWGEQWTPQTRWGKTILGLTLAIGPSAGVLGAAFGMF
                     GIRSGEQNSLILIAAVLFSAVAIQFAFAFAYQFWPERPWSKQAGSS"
     gene            complement(2938..5550)
                     /locus_tag="VI520_02875"
     CDS             complement(2938..5550)
                     /locus_tag="VI520_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36859.1"
                     /translation="MQPAVRPRTILLPRRALLVFVAFLFAAFLTLPALAEYLGPDRTT
                     TVFVEVRDPDHDVWTLTHVDPLDGLADVCLIIHTCDEHPSIERQQALCGWVADNSGCD
                     KAYKTEEQTVLLPEATIGGVLQNCSLVDGWCTSSPTLHLTAAEPVAGESITLIEGTRN
                     GESFACSGVVCDVPLLEGSNEFTFWALSTWGDSSQMGTLSALVDSFGPTLAMPDEWYI
                     WEPLAIGVEDGQVGVDRVKLTIDGGSFGNRVYEWTLGNLPSDFIWDRHFGEIIAPIGE
                     YPVSVKAWDLLGNSSSAAGLILIPAPEEPETDEDSFATFAEPASTEGPVPTAAPIAGG
                     SVVEPTATHEPVAAVLVAEPATSIESAGPGTAATSTKGGTGGLLWGAAALAAAASATA
                     YALSRRQDRLAEVAAKRKEAAKAASAEGFNARLGRLRSAALTRVAPIRAGMLAAAAAA
                     AKATQEIQERRKVQYRLTQNMARLERLGPPTTSMEQMVAWQSPDPELNESYQLGRLYS
                     TSAGIGEVGIAPGTETPTPPGYQPVQIGTPSPVGTSDRPTAAPIPIAPYPPGYIPYDP
                     ESVEGKWLGRGASLIYSGSRAYTASAIQYAALDGGRIVVVSAPSLARGTRASLLSRHF
                     LRGGPYTRTTLSQVGLRQFTLGNLVRGAKGSVGVGLLTSLATNLWDFTVGTQRETGIL
                     SKEFAVSTGVDLIMSVGTGLVAAGLVAGGAAAAAAVAGFTAPIWGTIAAVAVVGLVIG
                     LVLDSTGVGTTLKRNVSEGLEAWPGIIQNAGVIAQVMGGRVGDLASDAAQSVADRAVR
                     AADAVATVGREATRAVASTLHRVSEATRSSVDRASSAIQDAAGQAVSSIANEASQAID
                     SAREFVGNLFGGED"
     gene            complement(5738..6664)
                     /locus_tag="VI520_02880"
     CDS             complement(5738..6664)
                     /locus_tag="VI520_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36860.1"
                     /translation="MTPGELNPDRLFPSGTQRIYAVWDYSGMRAGLHVRREWYRDGEL
                     WLVREEPWDLGRYGSSGRIGDISIFDFDYGLIPGSYDLQLSIDGEFASRGGFFIWGYA
                     LDPVWSPDGASLAAVRRPGTLLLEESDGSTRELVEGVEISHLVWHPDGEHLLYAVIDR
                     PEERDPYVNTGRFELWTVDVLGEEAWRISLPGESLRDPLVSPGGGYAAAFSGSGFGDA
                     CFLDTQLVVLELGSDMRRIADFGLGDFLGVLPTPLDPSTIIFPFDRAWRSESQLEVSL
                     AEYCTGGEFESGRYLLDLETMTGQRIGDTGAP"
     gene            complement(7071..7757)
                     /locus_tag="VI520_02885"
     CDS             complement(7071..7757)
                     /locus_tag="VI520_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HLF36861.1"
                     /translation="MDKFEGFPAAGFEWFRKLAKNNNRPWFEKHKQEYVDYLQAPALA
                     MVVELGERLKSIIPGISYEARPSGGSLMRIYRDIRFSKDKTPYKTHIGINWWEETGKG
                     GAGYHFFMDAEGAVLYAGHHEFDKPYLTAYREAVADDRTGLALQRAIDTLTKSGGYEV
                     GGEQLKRVPPGYDQDHPRGDLLRYKGLWVRSPKMKPAELGSPKVIDACLKHCRAMLPV
                     HKWFAAVTAA"
     gene            7859..8563
                     /locus_tag="VI520_02890"
     CDS             7859..8563
                     /locus_tag="VI520_02890"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HLF36862.1"
                     /translation="MGQRYGNIVGWGKHVPERVLTNADLERMVDTSDEWIVSRTGIRE
                     RRVVSEGETTSGLAIEASRQALEMAGVRARDLGLIIVATSSPDYLTPPISSQVQHALG
                     AKDVGAFTLVAGCPGFVYGLATAQQFIATGACDTILVVGVEIISRFIDYTDRSTCVLF
                     GDGAGAVVVQVSEQPSGVLSFVLGSDGSGAQHLILPGGGTKARPPTKRSKPACSSCEW
                     TGSRCFGSRLGSWARR"
     gene            8524..8994
                     /locus_tag="VI520_02895"
     CDS             8524..8994
                     /locus_tag="VI520_02895"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HLF36863.1"
                     /translation="MFRFATRILGKALKQAIQQAGLTTDDIDLFIPHQANRRIIESAA
                     RYVGLPEEKVFINIDRYGNTSAASIPIALTEAIEQGRARPGDTLAFVAFGAGLTWAAS
                     VVKLGSPVQPLPVDRAWLKRLAWNPLGAVAAGLGRAVGAITGSVARLVGWVRQG"
     gene            9252..9572
                     /locus_tag="VI520_02900"
     CDS             9252..9572
                     /locus_tag="VI520_02900"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLF36864.1"
                     /translation="MTTQRKTSRPAALDHRTAGHVAELFRAFSDPSRVRLISLLAERE
                     MNVGALAEAVGISESAVSHHLRGLRQMRLVRPRKEGRQVFYVLDDDHVAALFRQGLDH
                     VQHG"
     gene            9599..11959
                     /locus_tag="VI520_02905"
     CDS             9599..11959
                     /locus_tag="VI520_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HLF36865.1"
                     /translation="MIEKTIELEIPLLLPGVKDEADGCLARLESALQDRKGLTRVHLE
                     RDRRPVTLCLHYEPQVISLEDVRRLAQRAGAEIMGRYHHLFVPIEGMDCSDCVGVIEH
                     SLGRIEGVLAVSVSYPTETMRVEYDARATDRRAIEKRIRSLGYGIPANEAQQRVQENR
                     ELLLGLLSGALLLAGWAGETFFGLSRGFAVLAYLGVYALSGSHIARHAWHSLRERQFD
                     TDLLMVAAATGAAILGKWADGALLLFLMSLGHALEERALDRARSAIRSLADLAPKTAL
                     VRRDGQEVELPVEQLALDDVVIIRPGARLPVDGVITVGESAIDQSPVTGESVPEDKTP
                     GEPVFAGSVNGQGALEVSVTRLAKDSTLARVMKMVEEAPTQKSPTQNMVERFEHRFVP
                     GVLIVTGLLIVVPPLLGAPFEVSFLRAMTLLVAASPCALALGTPASILAGIAQAARNG
                     VLVKGGVHLENLGRLKAIAFDKTGTLTQGQPELTDVVQLSEPGGQIANADDLLALTAA
                     VESRSGHPLAEAVVRAARIKGLVLPTVGMVESLTGSGMRASANGRAVLIGNRKLMEES
                     QVVLTEQTTQQIEELQAKGKTTMLVAVEGQLAGVFALADTLRPDAIAALKELRRIGVQ
                     ETVLLTGDNARIAAAIANQVGLTTFRSDLLPEDKVSEVRALNQQLGPTGMVGDGVNDA
                     PALASATVGIAMGGAATDVALETADVALMANDLTKLPFAVGLGRATRAVVLQNLLISL
                     SVISLLVLATLTGAIGIGGAVIVHEGSTLVVVFNALRLLGYRAKGG"
     gene            11968..12597
                     /locus_tag="VI520_02910"
     CDS             11968..12597
                     /locus_tag="VI520_02910"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HLF36866.1"
                     /translation="MTSGIVLDGLVIASAALVSVVVLASWLAALRAPRSPPVGSSRWF
                     RLPSWAQVGAGFGAVALFAYLGYRLWIPLPLRLPPPMISALRIAGLAAFLVGSALVLG
                     ARLSLGSMYGVSTSSAAQLRVSHNLIRRGPYARVRHPMYLGYWLLFAGALLIYRTWTP
                     LALLAMFVPAFYRRARREEAALAQRFGGEWDAYAARVPMFIPRWHSRTG"
     gene            12594..13436
                     /locus_tag="VI520_02915"
     CDS             12594..13436
                     /locus_tag="VI520_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLF36867.1"
                     /translation="MSAPLIGLLSALASALVWGGGDFVGGLAAYRRSPYQIVALASLA
                     GVPLLLFLAYLANERIPPPPDILWATAGGISGAVGVAALYRGLSTGSAAIVAPTAGVV
                     GAAIPVIVGSLLQGLPPGTRLAGITVGLVGIGLVSKAADPRVDSVRSSVSLSVLAGTG
                     FGGFFVFIAQLKGGLLFAPLVIAKLAAVALACLILVIRREPLPSPASQPIALLAGALD
                     AGGNFFYLLAVQLVRLDVAAVLTSMYPATTVILSAALLHERIRSLQMVGVGLCLTGLA
                     LIAL"
     gene            13495..14487
                     /locus_tag="VI520_02920"
     CDS             13495..14487
                     /locus_tag="VI520_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLF36868.1"
                     /translation="MHTHDPSTVDSHHDHHAIDPTILTSERGIWAIKWSFVGLMTTAL
                     LQVVIVYFTGSVALLADTIHNFGDASTAIPLWIAFRLARRKPSKRYPYGYGKVEDLAG
                     AVIVLLILFSAVIAGVQSVERFLNPQPVRYLWAIGAAAIIGFLGNEFVALFRIRVGRE
                     INSAALIADGYHARADGLTSLAVLGGAVGIWLGFPLADPIVGMLITVLILRIVWASAR
                     TVFGRMIDGVGPEVVDEIRHAALHIEGVEQVSEVRARWIGHRLHGEVNLAVVPALSVG
                     EAHGLGQAARHAILHHLPSLSNVIVHVDPADASGEDFHRIERHAHDELEPHFHP"
     gene            14531..14944
                     /locus_tag="VI520_02925"
     CDS             14531..14944
                     /locus_tag="VI520_02925"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HLF36869.1"
                     /translation="MPEISVAQFSDRFVALILGARDLPKKALDLNILLLSAVLALEVG
                     QAYSESELNAELQRWILEVGGKFGLDHVTLRRYLIDEKYLRRDPAGTAYRLDPAGPRF
                     TSDPAIRSLDLQVLVAEARQERQRRKQQHLQQSGP"
     gene            15141..15764
                     /locus_tag="VI520_02930"
     CDS             15141..15764
                     /locus_tag="VI520_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF36870.1"
                     /translation="MDTNQQEFKELYAAWQPKIHRYLVRLVGESEAEDLTQETFIKVS
                     RGLKGFRGEANLSTWIYRIATNAAVDRSRNASFRRTLVDAAPLEALEPAELEGVTRDG
                     WSGEQPPSPEQLTVRKAMNECIQDYVANLPEDCRAALVLSEMEGFKNREIAAILGVSL
                     ATIKIRLHRARAQLRVELERHCDPVWIEENEFLPDLKSALNGASRNS"
     gene            15823..16080
                     /locus_tag="VI520_02935"
     CDS             15823..16080
                     /locus_tag="VI520_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLF36871.1"
                     /translation="MVLDNHMLRLIAVGASVTANCLPCLEANISKAVENGAVESEIEQ
                     ALKVGRLVRQGAATKVDDFALSRIHATPGAVEPARAECGCG"
     gene            16102..16443
                     /locus_tag="VI520_02940"
     CDS             16102..16443
                     /locus_tag="VI520_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36872.1"
                     /translation="MEQTLDSLKVLNRRYEYTAWGFLLIWWGLVEWFGFLPEGAGPIG
                     TGVILLGLNAVRSLNGIRTNRFSMTVGILALVLGGLQLAGSRLGLALVIPLFPILLIV
                     LGVSLLLPRQS"
     gene            complement(16452..17111)
                     /locus_tag="VI520_02945"
     CDS             complement(16452..17111)
                     /locus_tag="VI520_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36873.1"
                     /translation="MEQADGNHADKPIRVLVADDHPVVRSGIKDELAKHPDLQLLGEA
                     VDGDQALELARTLRPDVLLLDINMPGLQPVKVVRELAKLDGGPQVLVLSAYSDVEYVF
                     EMLKAGVDGYLLKDEEPGRIAEGIRAVAAGKTWLSSAVAESIVASTVREGRQHELSPR
                     EEEVLSLMAKGLSNEAIGEQLSITVGTVKNHVSNLYSKLGVRTRAEAVAWAWVHRKRE
                     D"
     gene            complement(17123..>19131)
                     /locus_tag="VI520_02950"
     CDS             complement(17123..>19131)
                     /locus_tag="VI520_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLF36874.1"
                     /translation="TFWAIGLAVLSFGQAGAEARPFFRFCLIASGVLGAGSLSTAGPI
                     WSSTLFNILLWFAGPIAVDLHLRFPEARRHSRAMSAVLYGVAILGSLPFLLLGAAGVR
                     ASAWNAVVFSASRLFLSANLLLVVWLLLSSYRRATSVQARQRIRLVALGGGAGMLLVI
                     VLSILPGALTAGPLVPYEGSFAFLIAVPASYGYAIARHRLIRLERFLSRGAAYTLVFA
                     ILAATYLGVTTALDRLFSSELLEQPLINMLVILLLAATASVLYQRIQGLVDFAFYGGW
                     YDYRSAVARITNGLEALKSGEELAATLGARLETTLRLERACVFVPTRDGSLAPSDCDF
                     SGTADLSDPPTLPAQGEVVQYLKLNSRPLLAEALVEALQNRPLTKDERGVLQATHGSL
                     LVPVAGGEGLLALMALGRHRGGEGFSAEDLDILDLVARHAGAAIENVRLTGEVRRHVA
                     EVEQLHRRLLRAREEERKSLARELHDEAIQSLVGMNYRLAHLQTSQTQALRGEVLRIV
                     EDLRGMIHRLRPPALDNFGLVTAVRSELRERTVRSNGSLRIDLHVEGDPELGIPEEVG
                     LCLYRMIQEALNNVERHSEAGQVDVLLAMETPEIRLSVRDDGKGFPVPQHLGLLLEAD
                     HFGLVGLRERVELLHGTLEIDSAPGSGTTLRARIPLRTNPSSAA"
BASE COUNT         3600 a         6098 c         5950 g         3483 t
ORIGIN      
        1 aagcgcgagg acatatgcga ctgatagcgc cgtgacccaa ccccaagtcc cgggcaggat
       61 ctcggcccat actctgatcg cgatgagata catgagcaat gggtaggcct gcaaggtaag
      121 cagtcgcgac caccagctgt aagccttcca ggatcgaatc gtagatgcga tcccggccat
      181 ggccaccgtt gctccagccg ctgtcgctgt aggcgaaaac ctggggaaaa gagccagaat
      241 ggtggccaag ctcccaaagc tgagcactgc gacccctaca atctgccacc acggccaaga
      301 cgctgcggga gatcgattgc gacgagatgt catttaggtc gctcgaacct cccgacgaga
      361 ctgggcttga agtggtcaga gctgaattgg aggagaaaca gcttgggcta gggattcctt
      421 acggggggct attccttgtc aagtctcgtc cgctggccat tctccatgga gcggtcaatt
      481 ctccgccgac gaagccaacg agagagccgc agccccatcc atcctccgag agtgcccgcg
      541 atgaccataa gaaggaattg acgaaagtcc attttttgag actgttagga cgggcctgcc
      601 tgcttcgacc agggtcgttc gggccacagt acgtatgcat tcgcgaacgc gattccaatt
      661 gcggcgatcg atccgagtgt ggccagggcc agataaacaa gattcgcttc tccaaatctc
      721 gagccgtaca ttccgaaact agctccgagc actcctgccg aaggaccgac tgccagaacg
      781 acgcccataa tggcccggcc catacgagtc tgtggagtcc actgctcccg ccagagcact
      841 cccgatagga cggggttgag ggccggcaac aaccatgcca agacgtaggc cccaagaatc
      901 ggaactagcc agatccagac gatgggaatc aaatggctcc aacctctggc cgcaagcaat
      961 aggatttgca gggtccaaag caacaagatc gatagacgcg cccaccagtt gtaggtccac
     1021 caattccaca gcccaacggg aattgcaagc gcggctagtc caatgccgac ccaaagcgcc
     1081 gagatcgaga acggaggaat aagccccgga agcattccga atacccccgc ggcgaacaca
     1141 agggttggga acacctgcca gaggcgccag tgtgaaatgg gatgcctttg cgaccttcta
     1201 agcattagcc ttttggtttc gcttcagtcg ccctaagcca gctggtccaa gcgccccgtc
     1261 ccccatctga gtgaacagaa tgacagcctg gagagcagca ttcccatcca tcctccgaga
     1321 gtgcccgcga tgaccataag aaggagttga cggaagtcca tttctaagaa ggtcaggacg
     1381 agcctgcctg cttcgaccag ggtcgttcgg gccagaactg gtaggcgaag gcaaaggcaa
     1441 actgaatagc gactgctgaa aacaatacgg ccgcgatcaa gatcaaggaa ttctgttcgc
     1501 cagatctgat cccaaacata ccaaatgccg cgccgagcac gccagctgaa ggcccaattg
     1561 ccagagttag gccaagtatg gtcttgcccc aacgtgtttg gggcgtccac tgttcacccc
     1621 agagggtttc cgacaccaaa ggttgcatag cgggcattgc ccatgccaac agatacgctg
     1681 aaacgatcgg aaccagccag atccaggtga tcccgatgag cagcgtccat gccctagccc
     1741 ccgtaacaag gatctgaagg ctccacagag ctgctccagc gagtcgagcc caccagctga
     1801 agttccacca gctccatagc gcagccaacg cggcagggat agccaatgcc catccaagcc
     1861 gcatcgcaga gatcgacacc gggggaagga atccgggcag ggctccgaaa accccgaaca
     1921 ccaaaattgc gagactcaac accttccacc aagcccaaat gaccttagga tgacccttgc
     1981 ttctcttgga catcagctca ccctgccgcg aaggttgtct cgagcgctcg gtaagagaga
     2041 aggagccagg accgagatga ggattcaatg aagttcctcg cttggacctt ctacaagcac
     2101 gtgcccgtcc gcttgaccca aggccggtct ggccagactt ggtaggcgaa tgcgaaggcg
     2161 aagccgatcg cgaccgcaga agaaagtgcg gcaataacta gtatcaccga gtcggtttcg
     2221 ccgaatcggc ttccaaacat gccaaccgat gctcctatga cgccagccga ggggccgaca
     2281 gcgagagcga ggcctagcac agcccgtccc acgcgtgtcc ggggcgctat ctgctctcgc
     2341 cagaggatcg ccgaaagtcg aggatttagg gccggcatcg cccaggcgag aatgtaggca
     2401 gaaaggatcg gagccatcca cagccatgtg aacccaagca caagcgacca gcctctcact
     2461 gcgatcgcaa ggaattgaag tgtccagagt cctacagcag ccaggcgggc ccaccaacta
     2521 aatgtccacc aactccacaa cactgcgatt atcaccggta cggacaatag cagtccaaga
     2581 cgaattgttg aggaggagac cgggggtaag aatccgatca tggccccgaa gaaaccgaag
     2641 accactactg ccactggaac cgccttccac cacggccaat tggaggctgg acggcgctta
     2701 actcttttgg ccacctatta gcccgcttca gctcggcgct gagcgctcga tcggctagtc
     2761 accacgccgg gaaaagacaa catgctatgt tttcggtacg attgcctgtg cgtaggattc
     2821 atgggactga cttcaaattg agttcctttc gttgggcttg tcattcaagg ttgtagtcca
     2881 aatgccctgc cacagcaact caatacgacc ggcctcaccc tgtcttggga gagtgattca
     2941 gtcttcaccc ccgaaaaggt tgccgacaaa ctccctggca ctgtcaatgg cctgacttgc
     3001 ttcgttcgca atgctcgaga cagcttgccc cgcagcatct tggattgcgc tagaggctcg
     3061 atcaaccgag cttcgggttg cctcgctcac acggtgaagt gtgcttgcga cagccctcgt
     3121 ggcttcccgt ccgactgtcg ctacggcgtc cgcggcacga actgccctat cggctaccga
     3181 ctgcgccgcg tcggaggcta ggtcgccaac gcgcccgccc ataacctgtg cgatgacccc
     3241 ggcattctgg ataatgccgg gccacgcctc cagaccctca ctgacattgc gcttgagtgt
     3301 ggtacccact ccggtggagt caaggacaag cccgatgacc aacccaacaa cagcgacggc
     3361 ggcgatggtc ccccatatcg gggcagtgaa gccagcaacg gccgcggccg cagccgcacc
     3421 tcctgccaca agcccagcgg ctaccaagcc cgtgcctacg ctcatgataa ggtcgacgcc
     3481 tgtcgaaaca gcgaattcct tgctgaggat tcctgtttcc ctctgagtcc cgacggtgaa
     3541 gtcccacaag ttggttgcta gcgatgtcag caaacctact ccaacacttc ccttggctcc
     3601 acgaacgagg ttgcccaagg tgaattgtcg aaggccgact tgggagaggg ttgtccgggt
     3661 ataggggcca cctctcagaa agtgcctgga aagaagactc gcgcgcgttc cccgagcaag
     3721 ggaaggtgca ctcactacca ctatccttcc cccatcgagt gcagcgtact gaattgcaga
     3781 cgccgtgtag gctctgctcc ctgagtagat aaggctcgcg ccccttccga gccatttccc
     3841 ttccacggat tctgggtcgt acggtatgta ccccggggga tagggagcga tcggaatcgg
     3901 tgcggcagtt ggtcgatcgg aggtcccgac aggcgatggc gtaccgatct gaacaggttg
     3961 gtacccgggt ggtgtaggtg tttctgttcc gggagcgatg ccgacttcgc ctatcccagc
     4021 tgaggttgaa tatagccggc caagttggta gctttcattc aattcaggat caggagactg
     4081 ccaggccacc atctgttcca ttgaagtagt gggcggtccg aggcgctcga gccgagccat
     4141 gttttgcgtg agtctgtatt gaactttccg gcgctcctgt atttcttgag ttgctttggc
     4201 ggctgccgcg gctgcggcga gcatgccggc tcggatcggc gcaaccctcg tgagagctgc
     4261 tgaccgcaat cgccctagcc tcgcgttgaa tccttccgct gaggcggcct tcgctgcctc
     4321 tttccgcttt gcagccactt cggccaagcg gtcctggcgt cgtgacagtg cataggccgt
     4381 ggcggaagcc gcagccgcga gggcggcagc gccccagagc aaaccgcccg tacctccctt
     4441 ggtggaagtc gcggctgtgc ccggcccggc cgattcgatc gaggtcgcgg gttcagcaac
     4501 caagacagcc gccacaggct cgtgggtcgc cgtgggctca acgaccgatc cgccggcgat
     4561 cggggcagcg gtggggacgg ggccttccgt cgaggccggc tcggcgaagg tcgcgaaaga
     4621 gtcctcgtcg gtctcgggct cttcgggcgc cgggatcagg atcagcccgg cggccgagct
     4681 tgagttcccg agcaggtccc aggcctttac cgagaccggg tactcgccga tcggggcgat
     4741 gatctcgcca aagtggcgat cccagatgaa gtcactcggc aggttcccaa gcgtccactc
     4801 gtagacgcgg ttgccgaagc tgccgccgtc gatcgtgagc ttgacccggt caacgccgac
     4861 ctgtccatcc tccacgccaa tggccaaggg ttcccagatg taccactcgt caggcatggc
     4921 gagtgtcggt ccgaacgaat cgaccaacgc ggagagcgtc cccatctgtg agctgtcgcc
     4981 ccaggttgac agcgcccaga aggtgaactc gttcgaacct tcgagcagcg ggacgtcgca
     5041 cactaccccg gaacaggcga acgactctcc gttgcgggtg ccttcgatca gggtgatgga
     5101 ctcgccggcc accggctctg cagccgtgag gtgcagcgtc ggggagctcg tgcaccagcc
     5161 atcgaccagg ctgcagttct gcagcacgcc cccgatcgtc gcctcgggca ggagcacggt
     5221 ctgctcttcg gtcttgtagg ccttgtcaca gccggaattg tccgccaccc agccgcaaag
     5281 cgcctgttgc cgctcgatgc tggggtgctc gtcgcaggtg tggatgatca ggcaaacatc
     5341 agccagcccg tcgaggggat cgacgtgggt gagggtccat acgtcgtggt ctggatcacg
     5401 gacttcgacg aagaccgtgg tggtgcgatc cgggcctaag tactcagcga gcgcgggaag
     5461 ggtaagaaag gcagcaaaaa ggaaagcgac gaaaacgagg agagctcgcc gcggaagcag
     5521 gatggtccgc ggacgcactg cgggttgcat aatcaccccc gtgagtggag ccccctccag
     5581 accagccggg gtgttgccgg agtctcatca taagggagga atcgcggcga atcaacggct
     5641 gacttgtgaa agggggcaca aacaaggagg tgggcgccgc gcggcaggtc agctgatcag
     5701 aaagaggttc gtcacgcccc ccgagaattg accggatcta cggagcgccc gtgtcgccaa
     5761 tccgctgccc cgtcatggtc tccaggtcga gcaaatatct tccggattcg aactcgccgc
     5821 cggtgcagta ttccgccaga ctgacttcaa gctgagattc acttcgccat gcccgatcga
     5881 agggaaagat gatcgttgaa ggatccaacg gcgtggggag gaccccaagg aagtccccga
     5941 gcccgaaatc ggcaatccgt cgcatatcac ttcccagttc gagcaccaca agctgggtgt
     6001 caaggaaaca agcatctccg aagccggagc ccgaaaatgc cgccgcgtag ccgccgccgg
     6061 gggagacgag aggatctcgc agcgattcgc cgggcagtga gattcgccaa gcctcctcgc
     6121 ccagtacgtc gacggtccac agctcgaacc tcccggtatt gacgtatgga tcgcgttctt
     6181 ccggtcggtc gatcacggca taaagcaagt gctcgccgtc cgggtgccat acgaggtggg
     6241 agatttcaac cccctccacg agctctctcg tcgagccgtc agattcctca agcagcaagg
     6301 tccccgggcg gcgaacggcg gcaagcgacg cgccgtcggg cgaccacaca ggatcgagag
     6361 catagcccca aatgaagaaa ccaccgcggc ttgcgaactc gccatcgatg ctcagctgaa
     6421 ggtcgtagct cccaggaatg aggccgtaat cgaagtcgaa gatagagatg tcgccgatcc
     6481 tcccggaaga cccatatcga ccgagatccc atggctcctc ccgcaccagc cacagctcac
     6541 cgtcgcggta ccactcacgg cgcacgtgga gccccgctcg catcccgctg tagtcccaaa
     6601 cggcgtagat ccgttgggtc ccagaaggga acaaccggtc aggattgagc tcgcccggcg
     6661 tcattccaaa ctcaatgctc tcggagaacg acggtcgcgg cgggagctgc gtctgggtgg
     6721 gcctggcacc tgcagaagcc tccggggatg acttagccgt cgcggcctcg gtcggaaggg
     6781 gccgctcagc aggttcagac gccgagccag ccggtgtgga agcggattcg ctcgctggtg
     6841 cagcggactg cgcaggctga cctgcgccgc acccactgag cgtgggcacg gcgccaatgg
     6901 ctaggagtgc cagcagcatg gccggcgtga ggccagtgcg gcgggcgccg actacaaggc
     6961 cgaccagcct atgcatgctt gctgcggtga tggcacgctc gatcgaatcg cctcgaaagg
     7021 catcctcatg cggatcagag accgctagac taaacgaatc gggtgtcggg ttaggcagca
     7081 gtgaccgccg caaaccactt gtgcaccggg agcatcgccc ggcaatgctt caagcaggcg
     7141 tcgatcacct tgggtgagcc gagctcggcg ggtttcattt tcggagagcg gacccacaac
     7201 cctttgtagc gcagcaggtc gccccgcggg tgatcctgat catagccagg cggcacccgt
     7261 ttcagctgct cgcccccgac ttcgtagcca cccgacttcg taagcgtgtc gatcgcccgc
     7321 tgcagggcga ggccggtgcg atcgtcggcc accgcctctc ggtaggcagt caagtacggc
     7381 ttgtcgaact cgtggtggcc ggcatacagc accgccccct cggcgtccat gaaaaagtgg
     7441 tacccagccc cgcccttgcc ggtttcctcc caccagttga tcccgatgtg ggtcttgtag
     7501 ggggtcttgt ctttgctgaa gcggatgtcg cggtagatcc gcatcagcga gccgccgctg
     7561 gggcgcgcct cgtagctgat gcctggaatg atcgacttga gccgctcacc cagctcgacg
     7621 accatcgcca gagccggcgc ctggagatag tccacatact cttgcttgtg cttttcgaac
     7681 caggggcggt tgttgttctt cgccagcttg cggaaccact cgaatccggc agccgggaat
     7741 ccctcaaact tatccatgat cgcggctcct cctacctgcc cgcgattatt ccggagcggc
     7801 gaagcgccgt caaggttagc ccgcgatctg tggcagaatc gcactcagcg gaggccggat
     7861 ggggcagcgc tacgggaata tcgtcggctg gggtaagcac gtccccgaac gggtactgac
     7921 caacgccgac ctcgagcgga tggtcgacac cagcgatgag tggatcgtca gccggaccgg
     7981 gatccgcgag cggcgggtag tctcggaggg ggagaccacc tctgggctgg caatcgaagc
     8041 ctcccgccag gcgctcgaga tggccggcgt ccgggcccgc gacctcggcc tgatcatcgt
     8101 cgccacctcc agcccggatt acctcacccc gccgatttcc tcccaggtcc agcacgccct
     8161 cggcgccaaa gatgttggtg ctttcacgct ggtggccggc tgcccggggt ttgtctacgg
     8221 cctggcgacc gcccagcagt tcatcgctac cggcgcctgc gacacgatcc tggtggtcgg
     8281 ggtcgagatc atcagccgct tcattgacta caccgaccgc tcgacctgtg tgctcttcgg
     8341 ggacggcgcc ggcgcggtcg tcgtgcaggt cagcgagcaa ccctccggag tgctgtcgtt
     8401 cgtgctcggc tcggacggct cgggcgccca gcacctgatc ctccctggcg gtgggaccaa
     8461 ggcccgccca cccacgaaac gatcgaagcc ggcctgcagt tcttgcgaat ggacgggaag
     8521 caggtgtttc ggttcgcgac tcggatcctg ggcaaggcgc tgaagcaggc gatccagcag
     8581 gccgggctga ccaccgatga catcgacctc ttcattccgc accaggccaa ccggcgcatc
     8641 atcgagagcg cggcccgcta tgtcggcctg cccgaagaga aggtcttcat taatatcgac
     8701 cgctatggga acacttcggc ggcctcgatc ccgatcgccc tcaccgaggc gatcgaacaa
     8761 gggcgggccc gaccgggcga caccctggca tttgtggcct tcggagccgg gctgacctgg
     8821 gcggcctcgg tggtcaagct cggctcaccc gtccagccgc tgccggtcga tcgggcctgg
     8881 ctgaagcgac tcgcctggaa cccactgggc gccgtcgccg cggggctcgg ccgggcggtc
     8941 ggagccatca ccgggtcggt ggcgcggctg gtcggctggg tgcggcaggg ctagcgctcc
     9001 agccggagca gggcaacctc ctctattcgg gaaccggcct tcgtaaggac accccaaccg
     9061 agttgcggaa cccgggcctc gccacgttca ggggagcgga ccgatcgcac tccagcggca
     9121 gcctgcttgc cggttggtta ccgcggccct tgtacccgag ccgtgcccaa tcaaccatcc
     9181 agcgtcggcc caggttgaca gccgcagcac actggcgtat aatcggaata tctgagtata
     9241 tattcatgta aatgacgacc caacgcaaga cttccagacc cgctgccctt gaccaccgca
     9301 cagccggcca cgtcgccgag ctgttccggg cgtttagcga cccaagccgt gtccgcctga
     9361 tttcgctttt ggcagagcgc gaaatgaacg ttggcgcgct tgccgaggca gtcgggatca
     9421 gcgagtccgc cgtctcgcat catttgcgag ggctgcgaca aatgagattg gttcgaccgc
     9481 gcaaggaggg ccgacaagtc ttctacgtgc tggatgatga ccacgtggcg gctctgttcc
     9541 ggcaggggtt ggatcacgtt cagcatggct gagcggtgcg ccttagggac tccaaaccat
     9601 gatcgaaaaa acgattgaac tcgaaatccc cctgctcttg ccgggggtga aagatgaggc
     9661 ggacggctgt ctggcccggt tggaatcggc gctccaggac cgtaaggggc tgacccgggt
     9721 ccacctggaa cgcgaccggc gaccggtcac tctgtgtctc cattacgaac cacaggtcat
     9781 ttcattggaa gatgtcaggc gactggccca acgcgccggc gcagagatca tgggccgata
     9841 ccatcacctg tttgttccca tcgagggaat ggattgctcc gactgcgtcg gcgtgatcga
     9901 gcacagcctc ggccggatcg agggggtgct cgcggtcagc gtcagctatc caactgaaac
     9961 gatgcgggtc gagtacgacg cccgcgcaac ggaccggcgc gccatcgaga agcggatccg
    10021 ctcgctcggc tacgggattc cggccaacga agcgcagcag cgcgttcaag agaaccgcga
    10081 gctccttctc ggtctgctct caggcgcgtt actgttggca ggctgggcag gcgagacttt
    10141 cttcggcctc tcgagagggt ttgccgtgct ggcctatctc ggggtctacg cgttgagcgg
    10201 atcccatatc gcccggcacg cctggcattc gctccgtgaa aggcagtttg acaccgacct
    10261 gctgatggtg gcggctgcga ccggggcggc gatcctcggg aagtgggctg acggggcgct
    10321 gctgctcttc ttgatgagcc tgggccatgc gctggaggag cgggccctcg accgcgcccg
    10381 ctccgccatc cgatccttgg ccgatctcgc ccccaagacg gcgctcgtgc gccgtgacgg
    10441 tcaagaggtc gagcttccgg tcgaacagct cgcgctggac gatgtagtga tcatccgccc
    10501 gggggcccga ctgccggtgg atggagttat cactgtcggc gaatcggcca ttgaccaatc
    10561 cccagtcacg ggcgagtccg tgccggagga caagactccc ggcgagccag ttttcgccgg
    10621 atcggtgaac ggtcagggag cgttggaagt gagcgtgacc cggctggcga aggatagcac
    10681 gctcgcccgg gtgatgaaga tggtcgaaga agcacccacc cagaaatcgc ctacccagaa
    10741 catggtcgag cgcttcgagc atcgtttcgt gccaggcgtg ctgatcgtca cgggtttgct
    10801 catcgtcgtg ccgccgctgc tgggggctcc ctttgaagtc tccttcttgc gcgccatgac
    10861 cctgctggtg gcggcctcgc cctgcgccct ggcgctgggc acgccggcct cgattctggc
    10921 gggcatcgcc caggcggcgc gcaacggcgt gctggtgaaa ggcggcgtcc acctggagaa
    10981 cctcggccgg ttgaaggcga tcgccttcga caagaccggc accctcaccc agggccagcc
    11041 cgagctcacc gatgttgtgc agctctccga acccgggggt cagattgcaa acgcggacga
    11101 cttgctggcc ctcacagcag ccgtggagag ccgctcgggc catccactgg ccgaggcggt
    11161 ggtgcgggcg gcccggatca agggcctggt cctgcccacc gtgggcatgg tcgagtcgct
    11221 cactgggagc gggatgcgcg cctcggcgaa cggtcgggcg gtgctgattg gcaatcgcaa
    11281 gctgatggag gagtcgcaag tcgtcttgac cgagcaaacg acgcagcaga tcgaagagtt
    11341 gcaggcaaaa ggcaagacga caatgcttgt cgccgtcgag gggcagctgg ccggcgtatt
    11401 cgcgctggcc gacacgctgc ggcccgatgc catcgctgcg ttgaaagagc tgaggcggat
    11461 cggcgtgcag gagacggtgc tgcttacggg cgacaacgcc cgaatagctg cggccatcgc
    11521 caatcaagtc ggcctgacca cctttcgctc cgaccttctg ccggaagaca aagtgagcga
    11581 ggtccgcgcc ctcaaccagc agctcggtcc gactgggatg gtcggggacg gggtcaatga
    11641 cgcgccggcc cttgccagcg cgacggtggg aatcgccatg ggaggtgcag ccaccgatgt
    11701 ggccttggag accgccgacg tggcgctgat ggccaatgac ctgaccaagt tacctttcgc
    11761 ggtcggcctg ggccgggcga cccgcgcggt cgtcctgcag aacctgctca tatcgctcag
    11821 cgtcatcagc ctgctagtcc tggcgacgtt gaccggggcg atcgggatcg gtggcgcggt
    11881 catcgtccat gaagggagca ccctcgtcgt ggtcttcaac gcgctccgcc tgctcggtta
    11941 tcgagcgaag gggggatgaa cggatcgatg acgtcgggca tcgtgctgga cgggttggtg
    12001 atcgccagcg cggcgctcgt gagtgtcgtt gtcttggcca gctggctggc cgccctccgc
    12061 gccccgcggt cgcctcccgt cggttcgagc cgctggttca ggcttccctc atgggctcag
    12121 gtgggggccg gattcggggc ggttgcgctc ttcgcctacc ttggctaccg gctttggatt
    12181 cccctccccc tgaggctgcc tccccccatg atttccgccc tgcgcatcgc cgggttggcc
    12241 gcctttctcg ttggatctgc gcttgtgttg ggggcccgtc tgtcgctggg ctcgatgtac
    12301 ggtgtgagca cgagctccgc cgcccagctg cgggtgagtc ataacctgat ccggcgcggt
    12361 ccctacgcgc gagtgcgcca tccgatgtac ctggggtact ggctcctttt cgccggggcg
    12421 ttgctcatct atcggacctg gacccctctc gcgctgctgg cgatgttcgt gccggcgttc
    12481 taccgccgag cgcggcgcga ggaggccgcg ctagcccaaa ggtttggggg cgagtgggac
    12541 gcctacgccg cccgtgtgcc aatgttcatc ccgcgctggc attcccggac gggatgagcg
    12601 cgccactgat cggactgctc tcggctctgg cctcggcgtt ggtgtgggga ggcggcgact
    12661 ttgtcggtgg tctggctgct taccgccggt ctccctatca gattgtggca cttgcctcgc
    12721 tggcgggtgt tccgctcctc cttttccttg catacctcgc caatgagcgc atccctcccc
    12781 cgccggacat tctctgggcc acggccggtg ggatctccgg ggcagtcggg gtcgcggccc
    12841 tctaccgggg tctctcaacc ggctcggcgg ccatcgtggc ccccaccgca ggagtggtgg
    12901 gcgccgcgat cccggtcatc gtgggttctc ttctgcaagg ccttccacca ggcactcgcc
    12961 tggccgggat caccgtagga ctagttggga tcgggctggt cagcaaggct gccgatccgc
    13021 gagtcgattc agtcagaagc tcggtgtcat tatcggtttt ggcaggcacc gggttcgggg
    13081 ggttcttcgt gttcatcgcc cagcttaaag gcgggctgct cttcgcaccc ctggtcatcg
    13141 ccaagctggc cgcggtggcc cttgcctgcc tcatcctggt catccggcgg gaacctctgc
    13201 cttccccggc cagccaaccg atcgcgctgc ttgccggcgc gctcgatgcc ggtggtaact
    13261 tcttctactt gttggcggtg cagctcgttc gcctggacgt tgccgcagtg ctcacttcca
    13321 tgtaccctgc caccacggtg attctgtcgg ccgcactgct gcacgaaagg attcgctcac
    13381 tgcagatggt gggtgtgggg ctgtgcctga ccgggcttgc cttgattgct ctgtagcacc
    13441 tccatacccg atgttagact tcaccatgtg tgaagggatt ccggattaga accgatgcac
    13501 actcacgatc cttcgacggt cgactcacac cacgatcacc acgccatcga tcccacgatc
    13561 ctgaccagcg agcgtgggat ctgggcgatc aagtggtcct tcgtcggcct gatgaccact
    13621 gcactgctgc aagtggtcat cgtctatttc accggcagcg tagccttgct ggccgacacc
    13681 atccacaact tcggagatgc ctcgaccgcc atcccgctct ggatcgcctt ccgcctggcg
    13741 cggcgcaagc cttcgaagcg gtacccgtac ggatacggca aggttgagga cctggccgga
    13801 gcggtcatcg tacttcttat ccttttcagc gcggtgattg ctggagttca gtccgttgag
    13861 cgatttctca acccccagcc ggttcgatac ctatgggcaa ttggcgcggc ggcgatcatc
    13921 gggtttctcg gcaacgagtt tgtggcgctc ttccggatcc gggttgggag ggagatcaac
    13981 agcgcggccc tgatcgccga tgggtatcat gccagggcag atggcttgac cagtctagca
    14041 gttttgggag gggcggttgg gatctggctc gggttcccat tggcggaccc gatcgtcggg
    14101 atgttaatca ccgtgctcat cttgcggatc gtctgggcat cggcccggac ggtgttcggg
    14161 cgaatgatcg atggggtcgg tccggaagtt gtggatgaaa tccgccacgc cgcgttgcac
    14221 atcgaaggcg tggagcaggt atcagaagtc cgcgcccgct ggatcggcca ccggctgcat
    14281 ggggaagtca acctggctgt cgttccggcc ttgtcggtcg gcgaggcaca tggactaggg
    14341 caggcggcca ggcatgcgat cctgcatcac ctgccatcct tgtcgaatgt gatcgtccat
    14401 gtagatccag cggacgcttc aggtgaggac ttccatcgga tcgagcgcca cgcccacgac
    14461 gagttagagc cgcactttca cccctgacat tggccgcgat gtcgcgggta ggagcatcct
    14521 gatcgccacc atgcccgaga tcagcgtagc ccagttctcc gaccgcttcg tggccctgat
    14581 ccttggcgca agagaccttc ccaagaaggc tctcgacctc aacattcttc tgctcagcgc
    14641 ggtcttggcg cttgaggtag ggcaggcgta ttccgagagc gagctcaacg ccgagctgca
    14701 gagatggatc ctggaggtcg gcggcaagtt cggcctggat catgtgacgc ttcgcaggta
    14761 tctcatcgac gaaaagtacc tccggcggga tcccgccggc acggcctatc ggctcgaccc
    14821 agccgggcca cgctttacct ccgatccagc gatccggtct ctcgacctcc aggtgctggt
    14881 cgccgaagca aggcaggaac gccaaaggcg aaagcagcaa caccttcagc agtccgggcc
    14941 gtaacgggca ggtccactaa cgagccaatt gagtcctcgc tgaccggcct tggccagcgc
    15001 tcggccgcca aagcatccgc acccccaatc aggacaaaca tcactcccct tgaccgtgag
    15061 gtcgatgctt aattatggag ggagccgtac cctggcccct gttatccgtg gccggctcgg
    15121 gtccaaccgg agaacaagga ttggatacca accagcaaga gttcaaagag ctctacgcag
    15181 cctggcagcc gaagattcac cgatatctgg tccggctggt cggcgagagc gaggccgaag
    15241 acctgaccca ggagaccttc ataaaggtga gtcgggggct gaagggcttc agaggcgagg
    15301 ccaatctctc gacctggatc taccggatcg ctacgaatgc cgccgtcgac cggtcgcgca
    15361 acgcgtcgtt ccggcgcacg ctcgtggatg cggctcctct cgaggccctg gagccggctg
    15421 aactcgaagg cgtgaccagg gatggctgga gcggtgagca accaccctcg cccgaacaac
    15481 tgaccgtccg caaggcaatg aacgaatgca tccaggacta tgtagccaac ctgccggagg
    15541 attgccgggc ggcgctcgtc ctcagcgaaa tggaaggatt caagaacagg gagatcgccg
    15601 ccatcctggg agtttcgctg gctacgatca agatcaggtt gcatcgggcc agagcgcagc
    15661 tgcgggtgga actggagcgg cactgcgacc cggtctggat tgaagaaaac gaattcctgc
    15721 ccgacctcaa gagtgccctg aacggggctt cccgcaactc ctagacctgt atccttcctc
    15781 ggtcgatctc cgtctatacg ggtagaacgg aggtgaattc ccatggtttt ggacaatcac
    15841 atgctgaggt tgatcgcggt cggagcatcg gtcacggcca actgcctgcc ctgcctggag
    15901 gccaacatct ccaaggctgt tgagaacggg gcggtcgagt ccgagattga acaggcgctc
    15961 aaggtcggca ggctggtacg gcagggcgca gccaccaagg tcgatgactt cgccttgagc
    16021 cggatccatg ccacgccggg cgcagtcgag ccagcgcgcg ccgagtgcgg ctgtggctag
    16081 ctgagaagga gagcgctcac gatggaacaa acccttgaca gcttgaaagt actgaaccgc
    16141 cgttacgagt acacggcatg ggggttcctg ttgatctggt ggggcctggt cgagtggttc
    16201 ggcttcctgc ccgaaggggc cggcccgatc ggcaccggag tgatcctgct tggtctgaat
    16261 gccgtgcgct cattgaatgg cattcgcacc aaccggtttt cgatgacagt tgggatcctt
    16321 gcgctggtgt tgggtggact gcagctggca ggatcgcggt tgggcttggc gcttgtgatc
    16381 ccgctcttcc ccatcttgtt gatagtcctg ggcgtatcgc tgcttctgcc caggcaatcc
    16441 taatccgggc ctcagtcctc ccgcttgcga tgcacccagg cccaggccac ggcctcggcc
    16501 cgggtgcgca ctcccaactt gctgtacagg ttgctcacgt ggttcttgac cgtgcccacc
    16561 gtgatcgaga gctgctcgcc gatcgcctcg ttgctcaagc ccttggccat caggctaagc
    16621 acttcctctt cccgcgggct gagttcgtgc tgccggccct cgcgcaccgt gctcgccacg
    16681 atgctctcgg ctaccgccga gctcaaccag gtcttgccgg ccgccacggc ccggatgcct
    16741 tcagcgatcc gcccgggttc ctcgtccttc aatagatacc cgtccactcc ggccttcagc
    16801 atctcgaaca catactcgac gtcgctgtag gcgctgagca ccaggacctg tgggccgccg
    16861 tccagcttgg ccaactcccg caccaccttc accggctgca gcccgggcat gttgatgtcg
    16921 agcagcagga cgtccggccg cagcgtgcgg gcgagctcca gggcctgatc gccgtccacc
    16981 gcctccccaa gcagctgcag gtccgggtgc ttggccaact cgtctttgat gccgctgcgc
    17041 accaccgggt gatcatccgc caccagcacc cggatcggct tgtcggcgtg gtttccgtct
    17101 gcttgttcca tggggatcgc cgtcaggccg cgctgctcgg attcgttcgg agtgggatcc
    17161 gcgcccgcag tgtcgttccg ctgcccgggg ccgaatcgat ctccagcgta ccatggagca
    17221 attccactcg ctctcgcaac ccaaccagac cgaagtgatc ggcctccagc agcagcccca
    17281 ggtgctgagg gaccggaaac cccttcccat cgtcgcgcac tgacagccgg atctcgggtg
    17341 tttccatcgc cagcagtacg tcgacctggc cggcttctga atgtcgctcg acgttgttca
    17401 gcgcctcctg gatcatccgg tacaggcaca gcccgacctc ctccgggatc ccgagctcag
    17461 ggtcgccctc gacgtgcaag tcgatccgca gcgatccgtt cgaccggacg gtccgttccc
    17521 gcagctccga gcggaccgcc gtgaccaaac cgaagttgtc gagcgccggc gggcgcaacc
    17581 ggtggatcat cccgcgcaga tcctccacaa tccgcagcac ctctcccctc agcgcctgag
    17641 tctggctggt ctgcaagtgc gccagccggt agttcatacc gaccagcgac tggatggctt
    17701 catcgtgcag ctcacgggcc agcgacttac gctcttcttc gcgagcccgc agcagtcgcc
    17761 tgtgcagctg ctcgacctcg gccacatgcc gccgcacctc gccggtgagc cgtacgttct
    17821 cgatggcggc cccggcgtgg cgggcgacca gatcgagaat atccaggtct tcggcgctga
    17881 atccctcccc gccacggtgg cgcccgaggg ccatcagcgc caacagaccc tcgcccccgg
    17941 cgaccggcac caacaggcta ccgtgcgtcg cttgtaatac gccgcgctcg tccttggtga
    18001 gcggccgatt ttgcagggcc tcgaccagag cttcggctaa gagcggccgt gagttcaact
    18061 tgagatactg caccacctcg ccctgggccg gtaaggtcgg cgggtcggag agatctgcgg
    18121 tgccggaaaa gtcgcagtcg ctcggggcca gcgatccgtc ccgggtcggg acaaacacgc
    18181 aggcccgttc gaggcggagg gtggtctcaa gtcgggcgcc cagggtggcc gccaactctt
    18241 ctccgctctt gagcgcttcc agaccattgg tgatccgggc gaccgccgag cggtagtcgt
    18301 accagccgcc gtaaaacgcg aagtcgacca gcccctggat ccgctggtag agcacgctcg
    18361 cggtcgcggc caatagcagg atcaccagca tgttgatgag tggctgttcg agcagttcac
    18421 tggaaaaaag tcgatcgagt gctgtcgtta ccccgagata ggtcgcagcc aaaatcgcga
    18481 acactagcgt gtaagcggcc ccgcggctca ggaaccgttc cagccggatc aggcggtggc
    18541 gggcgatggc gtagccgtag gaggccggca ccgcgatcaa gaaggcgaag gagccttcgt
    18601 aggggacgag cggcccggcg gtcagcgccc caggcagaat gctaagcacg atgaccagca
    18661 gcattcctgc gcctccgccg agcgccacca aacggatccg ctggcgggcc tgtacgctgg
    18721 ttgcccggcg gtacgagctg agcagcagcc acacgaccag cagcaagttc gccgagagga
    18781 aaagccggct ggcgctgaac acaactgcgt tccaggcact ggccctaacg ccggccgcgc
    18841 cgagcagcag gaacggcagg ctgcctagga tcgccactcc atagagcacc gccgacattg
    18901 cacgcgaatg ccgccgggcc tcagggaacc gcaaatgcag atcgaccgcg atcgggcctg
    18961 cgaaccagag caggatgttg aagagggtcg agctccatat cggtccggcg gtcgagagcg
    19021 aaccggctcc caacacgccg cttgctatca agcagaaccg aaagaaaggc cgggcctcgg
    19081 ctccagcctg gccgaacgac agcaccgcca acccgattgc ccagaaggtc g
//
LOCUS       DATDPQ010000123         3149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2399919, whole genome shotgun sequence.
ACCESSION   DATDPQ010000123 DATDPQ010000000
VERSION     DATDPQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399919"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1917
                     /gene="lon"
                     /locus_tag="VI520_02955"
     CDS             <1..1917
                     /gene="lon"
                     /locus_tag="VI520_02955"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HLF36875.1"
                     /translation="QKLNGLLSREIEVLSLGQQIQEEAREEIEKVQREYFLREQLKAI
                     QRELGEVDEQAAEVEEFRAKLEKAGLSPEADKMARRELDRLAKLPTAAAEYGVIRTYL
                     DWMVTLPWGKLTKDKLDLQNARQVLDEDHYGLQDIKGRILEYLAVRKLKIEREDLKSD
                     IPQDQIRREREGAILCFIGPPGVGKTSLGRSIARATGREFIRISLGGVHDEAEIRGHR
                     RTYIGALPGRILQALRRVETNNPVFMMDEVDKLGRDFRGDPASALLEVLDPEQNSEFR
                     DHYLEVAYDLSKVMFITTANWLDTIPPPLLDRMEIIRLSGYTEGEKAIIAKDYLIRRQ
                     IRENSLREAEVSFSDAAVREIIRSYTREAGVRSLEREIGKACRKIATRIAEGKIERLE
                     VTPEALPDLIGKRRFLGPEEIAERTQLPGVAAGLAWTPYGGDVLFIEATAMAGGRDFK
                     LTGSLGEVMKESAQAALSYVRSASKELGIRPDFWTTHDLHLHIPSGSQPKDGPSAGIT
                     MASALASLASGRPVRHDVGMTGEITLRGKVLPIGGVKEKVLAAHRAELKIIILPKRNE
                     VDLDEVPEEIKQEIEFIFVDNVSEVLKTALEAGTRRRARPKTGGNSGNSSRAKRSLKR
                     ARASRNAKAPDRRR"
     gene            1889..2242
                     /locus_tag="VI520_02960"
     CDS             1889..2242
                     /locus_tag="VI520_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36876.1"
                     /translation="MPKLLIVDDDPTMVSLLKTLLELDGFEVTPSSNPDQVMESIRQV
                     RPDLVIMDVFMSSRDGLELLRQVRASAELGHTPVIMTSGMDVSERAQEAGADGFMLKP
                     YSPPDLIALIRKKLS"
     gene            2268..>3149
                     /locus_tag="VI520_02965"
     CDS             2268..>3149
                     /locus_tag="VI520_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36877.1"
                     /translation="MSGARRWVAADLHLHTPASGDYQEPEVSYLDILRTALGRGLQII
                     ALTDHNTVAGYRRIREELEQLELLEKLGRIRNDERERLTEFRRLLTELLVLPGFEFTA
                     TLGFHILGIFPPDKPLRELDHLLLSLNIDPAELDSGSATVGASSDVLAAYRRINEAGG
                     LAIAAHANSTNGVAMRGFPIGGQTKIAYTQDPNLHALEVTDLEPRGRRSTADFFNGTK
                     PEYPRRMHCIQGSDAHRLRTDPRNPKNLGVGDRATNMMLQEVSFESLRELFLSNDFAR
                     TRPYQERRVEEFDFIRQA"
BASE COUNT          703 a          910 c          979 g          557 t
ORIGIN      
        1 caaaagctca acggtttact gagccgagaa atcgaggtgc tctcgctcgg gcagcagatc
       61 caggaggagg cccgagaaga gatcgagaag gtgcagcgcg agtacttcct gcgcgagcag
      121 cttaaggcga ttcagcgcga attgggcgaa gttgacgagc aagcggccga ggtcgaagaa
      181 ttccgcgcca aactcgagaa ggccggactt tcgcccgagg cggacaagat ggcccggcgc
      241 gaattggatc gactcgccaa acttccaacc gcggccgccg agtatggagt gatccggacc
      301 tacttggact ggatggttac gcttccctgg ggcaagctga ccaaagacaa actggatttg
      361 cagaatgccc ggcaggtgct cgatgaggat cactatgggc tccaggacat caaagggagg
      421 atcctggagt atctggcggt ccgcaagttg aaaatcgagc gcgaggatct gaagagcgat
      481 ataccccagg accaaatccg ccgggagcgg gagggagcca tcctctgctt catcggaccg
      541 cctggggtcg gaaagacctc gctcggcagg tcgatcgccc gtgccactgg gcgtgagttc
      601 attcgaatct cactcggcgg ggtgcacgac gaggccgaga ttcggggaca tcgcagaacc
      661 tacatcggtg cgctgccggg gagaatcctg caggccctgc gccgggtcga aaccaacaac
      721 ccggtcttca tgatggacga ggtcgacaaa ctggggcggg acttccgggg tgatccggcc
      781 tccgcccttc tggaagtgct cgacccagaa cagaatagcg aatttcgcga tcactacctg
      841 gaagttgcct atgacctttc caaggtgatg ttcatcacca ccgccaactg gctcgatacg
      901 attccacctc cattgcttga ccggatggag atcatccggc tatccggcta taccgagggg
      961 gagaaggcca ttatcgccaa ggactatctg atccgacggc agattcggga gaatagcttg
     1021 cgggaagcag aggtcagttt cagcgacgcc gcggtccggg agattattcg gtcttacacc
     1081 cgggaagccg gagtgcgaag cctcgaacgc gaaatcggga aggcctgccg caagatcgcc
     1141 acccggattg cagagggaaa gatcgagcga ctcgaggtca cccccgaagc attgcccgat
     1201 ctgatcggca agcgtcggtt cttggggccc gaggaaatcg ccgagcggac ccagctgccc
     1261 ggggtcgcag ccggcctggc ctggaccccc tacggcggtg atgtcctttt catcgaggcg
     1321 accgccatgg ctggcggccg agatttcaag ctgaccggat cactgggtga ggtgatgaaa
     1381 gagtcggccc aggccgcgtt atcctatgtt cgctccgcct cgaaggaact tgggattcgg
     1441 ccagattttt ggaccactca cgatctccat ctgcacatcc cgtcgggctc acagcccaag
     1501 gatggcccct ccgcagggat cacgatggcc tcggcgctcg cctccctagc aagcggtcgg
     1561 cccgtccggc atgacgttgg aatgaccgga gaaatcacgc tgcgcggcaa ggtgctgccg
     1621 atcggtgggg tgaaggagaa agtcttggcg gcccaccggg cggaactgaa gatcatcatc
     1681 ctaccgaaac gcaacgaagt tgatctcgac gaggtgccgg aagagatcaa gcaagagatt
     1741 gagttcatct ttgtagacaa cgtatcagag gtgctgaaga ctgccctgga ggccggaacg
     1801 cggcgacggg cacggccaaa aacaggagga aactctggga actcaagccg agcgaaacgt
     1861 agtctcaagc gcgcccgagc gagcaggaat gccaaagctc ctgatcgtcg acgatgaccc
     1921 cacgatggtc tcgctgctca agaccctgct tgagctagac gggttcgagg tcactccgtc
     1981 atcgaatccg gatcaggtca tggagagcat ccgccaagtg aggccggatc tggtaatcat
     2041 ggacgtattc atgagcagcc gggacgggct ggagctcttg cggcaggtca gggcctcggc
     2101 cgagcttgga cacacaccgg tcatcatgac ctccgggatg gatgtgtctg agcgggccca
     2161 ggaggcaggg gccgacggct tcatgctgaa gccgtattca ccaccggacc tgatcgcact
     2221 gatccggaag aaactaagtt gagttcgacc agatagggga ggacgatatg tcgggcgcaa
     2281 gacgctgggt cgcggcagac cttcaccttc atactccggc ttcaggagac tatcaggagc
     2341 cggaggtttc gtacttggat atcctgcgaa ccgccctggg gcgggggctg caaatcatcg
     2401 ccctgaccga tcacaacacc gtggccggct atcggcggat tcgggaggaa ctcgagcagc
     2461 tggagttgct cgagaaacta ggacggatcc gcaatgacga gcgggaacgc ctgacagagt
     2521 tcaggcgact gctcactgaa ttgctggtat tgccgggatt cgaattcacc gccacccttg
     2581 gctttcatat cctgggtatc tttccgccgg acaagccgct ccgcgagctg gaccacctgc
     2641 tgctctcatt aaacattgac ccagcagaat tggattcggg atccgccacg gtgggcgcct
     2701 ctagcgatgt attggcagcc tacagacgga tcaatgaagc cgggggtctg gcgatcgcgg
     2761 cccatgccaa ttccaccaat ggggtcgcca tgcgcggctt cccgattgga ggccagacca
     2821 agatcgccta tacccaggat cccaatctgc atgcgctgga agtcaccgac ctcgaaccgc
     2881 gcgggcgccg gtcgaccgca gatttcttca acggcaccaa acccgagtac ccccgtcgga
     2941 tgcactgcat tcagggctcg gatgcccacc gccttaggac agacccgagg aatccaaaga
     3001 acctcggcgt aggggatcga gcaacgaaca tgatgctgca ggaagtcagc tttgaatccc
     3061 tgcgcgagct cttcctcagc aacgactttg cccgcacccg accttatcag gaacggcggg
     3121 tcgaggagtt tgatttcatc cggcaagcc
//
LOCUS       DATDPQ010000124         6701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1102006, whole genome shotgun sequence.
ACCESSION   DATDPQ010000124 DATDPQ010000000
VERSION     DATDPQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102006"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VI520_02970"
     CDS             <1..538
                     /locus_tag="VI520_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLF36878.1"
                     /translation="PSFKGAMLTGDEVIELLNLQPLAVEGGYFRRSYRAADTLSADAL
                     PGRYRRDKALCTAIFYLLTADPDSFSALHRLPTDEIYHFYLGDPVELLLLYPDGDSAV
                     VRLGPDLRAGQQVQFVVPAGVWQGSRLAAGGSYALLGTTMAPGYDEGDYTGGDRGELS
                     ERYPDRAQLIWGLTRSTG"
     gene            complement(620..2197)
                     /locus_tag="VI520_02975"
     CDS             complement(620..2197)
                     /locus_tag="VI520_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF36879.1"
                     /translation="MRSNEDSAMTPEALRVQGITKRFPGVVANDTIDFALAQGEIHAL
                     LGENGAGKTTLMNVVYGLYKPDEGQVFINGNPVKIQNPKEALARGIGMVHQHFMLIPV
                     MTVAENLMLGHEITTGLRLGPLAKVDRRRVSQEITDLARRYGLEVDPEAQVHQLSVGE
                     QQRVEIVKALYRGAEVLILDEPTAVLTPQETEDLFAVMRSLIEQGKSIIFITHKLKEV
                     LAIATRITVLRDGKVVGTTEPGKTTEAELARMMVGREVLLRVDKKPAQPGEPVLIVED
                     LEVVDARERTVVRGVSFSVRAGEVLGIAGVQGNGQAELVEALTGLAPVVAGKIQLMGK
                     EVTHSRPRIFTETGAAHVPEDRHKHGLVLAYPVDENLILQTYYLAPFASGLNINPKAV
                     DANARRLVREFDVRTPSIETHAGSLSGGNQQKVIVARELSREVHLLIANQPTRGLDVG
                     SIEYIHHRIIEKRDEGVAVLLVSSELEEILNLSDRIAVMYAGEILAVIPAEQATAERV
                     GLLMAGVREPEAALAAS"
     gene            complement(2184..3320)
                     /locus_tag="VI520_02980"
     CDS             complement(2184..3320)
                     /locus_tag="VI520_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36880.1"
                     /translation="MDSVFFIGLLSLALVKATPLALGAMCGLMCERSGVINIGIEGMM
                     LMGAMMSFLGSVLFNDLTGGELPQLISLLVGLLVAMLSAGLIALLHARLSIHYKVDQI
                     ISGTVINLLAVGITNFTANAYIDPNHISGVGIFPVLEIPILSKIPILGPIFFSHQPLV
                     YVMLILVAVLQFALFNTPWGLRTRAVGEHPRAADTVGINVLRTRYINVTLGGVLAGMA
                     GAFLVLQSVGRFQKLMTTGRGFIALAALIFGKWTSVGALASALLFGFSEALGVRLQLG
                     DVNELEAFTLLSGIGITLLGVVWLIAKLVRGKDDKRSLALVIATPVVGAAMVASGLLA
                     TFPKIEIPIQFLGLLPYILTILVLAGVVGRAVAPAAIGKAYEKQ"
     gene            complement(3326..4450)
                     /locus_tag="VI520_02985"
     CDS             complement(3326..4450)
                     /locus_tag="VI520_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36881.1"
                     /translation="MTEAIEAAPRSRNLIGLILGRISIPVLAIFTALLVSSLVMIVSG
                     TNPIEAYLALAEGAFGTQVAVVETLIKATPFILAGLGIALAFRGGLFNIGMEGQLFVG
                     SVAAVLVGYGVELPPVIHLIVALLAGMLGGAIWAAIPGYLKARTGAHEVITTIMTNFI
                     ALRVINWAIGVDGPMRAPRSVVPETRAVFETARLPLLIPDSRLHAGIILALLAAIAVY
                     WLLWRTVIGFEIRTVGANPSAARYAGISVELNIVRTMALSGALGGLAGAIQVLGLAPY
                     NFTTGFNVGYGFDSIAVAVLGSIHPFGVTLAAILFGAMDAGARLMQLRTKVPIDIITI
                     VQGLILTFVAANQIIRAIYWIRVRAEEGPVRLSELWAGRD"
     gene            complement(4585..5739)
                     /locus_tag="VI520_02990"
     CDS             complement(4585..5739)
                     /locus_tag="VI520_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLF36882.1"
                     /translation="MKKTYAILFLLTAMAFVLGACATATPTEEVVAPPPAAEEPTAEP
                     PTAVPPPPPAECAHKIGFVTDVGKLNDQSFNEAGYAGVVSAAEQLGLGEECYGFIETA
                     DSADYTPNMESFIAEGFDIIVTSGFAMGAATHQAGKDHPDVFFIGTDQNQVDANFAPE
                     PLTNVTGLIFHEDVSGFLAGALAGLMTESNVVAGVYGCPFIPPVARFEVGYYNGAHYV
                     NPDVTVLNVYHPGSLDVCFVDPEFAAETAATLITEGADVMFGAGGLTGNGALIGACNQ
                     GVKVIGVDVDQFITLPEVADCIMSSATKALTSDVAALIISVDEGTFPGGEVFGPAVLA
                     PFHNFEDVIPQDVKDQLAEISAMIDSGAIDPCAPFEGSNAGTFCTPVAAP"
     gene            complement(5936..>6701)
                     /locus_tag="VI520_02995"
     CDS             complement(5936..>6701)
                     /locus_tag="VI520_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36883.1"
                     /translation="VLTSDPEGASAIVGEARVDLQAIVSFGEHFGGRATQIAADVAPL
                     VLVEAGQIEPGPRLSTVGEPGGRHDQAGFLAGVMVGLAGQTGWVGLVESTGGPLEPVY
                     RGGFEAGLRYGCPKCRLVTSPASEATVDGFRAQGVEAVFIIPGPAAAEAGAQLAEGGL
                     WLVWVGDLPTPDLRVAGQVVFAADGLILKALDALQAEQFGAAWPYAIENGGIRLGEVN
                     PEAISPGRQRLLQEAYEAVAGGQLDLGIDPATGQEG"
BASE COUNT         1291 a         2203 c         1989 g         1218 t
ORIGIN      
        1 accgtctttt aaaggagcca tgttgactgg agacgaagtt atcgagttgt tgaatctgca
       61 gccgctggcg gtggaaggcg gctacttccg gcggagctac cgggcggcag acacgctttc
      121 ggccgatgcc ctccccggcc gctatcggcg agacaaggcc ctttgcaccg ccatcttcta
      181 tctgctcacc gccgacccgg actccttctc ggcgttgcac cggctgccta ccgacgagat
      241 ctatcacttc tacctgggcg atccggtaga actgctgctg ctctacccgg atggagacag
      301 cgcagtcgtt cgactcggtc ccgatctgcg ggccggccag caggtgcagt tcgtcgttcc
      361 ggcaggggta tggcaaggct ctcgcctcgc ggcggggggt tcttatgcct tgttgggtac
      421 caccatggcg ccgggttatg acgaggggga ctacaccggc ggcgatcgag gggagctgag
      481 tgaacgctat ccggatcggg ctcagctcat ctggggactg acccgctcta ctggttagct
      541 ccccacgggg aactgcagca ggtcgagctc gccacagccg ggcagccacc ggcgcggcga
      601 ccggtcgagc tcgcctcagt taagaggcgg ccagcgccgc ttcaggttcc ctgacccccg
      661 ccatcagcaa cccaacccgt tcggcggtcg cctgctcagc cggaatgacc gccaggattt
      721 cccccgcata catcacggca atccggtcgg agagattgag aatttcctcc agctcggaag
      781 acaccaacaa gaccgccacc ccttcatcgc gcttctcgat gatccgatgg tgaatgtatt
      841 cgatcgagcc gacgtccagc ccgcgggtcg gctgattggc aatcagcaga tgcacttcgc
      901 ggctcagctc gcgggcgacg atgactttct gctgattgcc ccccgacagg ctgccggcgt
      961 gagtctcgat ggaaggagtc cgcacatcga attccctgac caatcgccgg gcgttcgcgt
     1021 cgaccgcctt gggattaatg ttaagcccgc tggcgaatgg agccaggtag taggtctgca
     1081 gaatcaggtt ttcatccacc gggtaggcca gcaccaggcc gtgtttgtgg cggtcttcgg
     1141 ggacatgggc tgccccggtc tcggtgaaga tccggggccg ggagtgggtg acctccttac
     1201 ccatcagttg gatcttcccg gcgacaaccg gcgccagtcc ggtgagcgcc tcgacgagct
     1261 ccgcctggcc attcccttgt accccggcaa ttccaagcac ctcgcccgcc cggactgaga
     1321 acgagactcc ccgcactacg gtccgctctc gggcatccac gacttcgaga tcctctacga
     1381 ttaggaccgg ctcgccgggc tgggccggct tcttgtccac ccgcagcagg acctcccgtc
     1441 ccaccatcat cctggccagc tctgcttcgg tcgtcttgcc gggttcggtg gtgcccacca
     1501 ccttcccatc ccgcaggacc gtgatccggg tcgcgatcgc cagcacttcc ttgagcttat
     1561 gggtgatgaa gatgatggac ttgccttgct cgatcagcga acgcatgacc gcaaagaggt
     1621 cttcggtttc ctgaggagtg aggaccgccg tcggctcatc caggatcaag acttccgccc
     1681 ctcgatagag cgccttgacg atttccaccc gctgctgctc gcctaccgag agctggtgca
     1741 cctgggcctc cggatcaacc tccagtccgt agcgccgcgc caggtccgtg atctcttgag
     1801 agacccggcg tcgatcgact ttggccaacg ggcccaaccg gagaccggtg gtgatttcgt
     1861 ggcccagcat cagattctcg gccacggtca tgaccgggat cagcatgaag tgttggtgga
     1921 ccatcccgat cccgcgggcc agtgcttctt tgggattctg aatcttgacc gggttgccgt
     1981 tgataaagac ctgtccctcg tccggtttgt agagaccgta tacgacattc atcaaagttg
     2041 tctttccggc cccgttctcc ccaagcagcg cgtggatctc gccctgggcg agggcgaagt
     2101 ctatggtgtc gttggcgaca accccaggaa agcgcttggt gatcccctga acccgcagcg
     2161 cctcgggggt catcgctgag tcctcattgc ttctcatagg cctttccgat ggccgccggc
     2221 gcgacggccc ggccgaccac gcctgccaag accaggatcg tcaggatata gggcaggagg
     2281 cccaagaact ggatgggaat ctcgatcttc ggaaacgtgg ccagcaggcc agaggccacc
     2341 atcgccgcgc cgacgaccgg cgtggcaatc acgagagcca gggagcgttt gtcatccttg
     2401 ccccgcacaa gttttgcgat cagccacact acacccaaga gggtgatccc aatcccgctg
     2461 agcagagtga aggcctcaag ctcattgacg tcacccagct gcaatcggac gcccagggct
     2521 tccgagaagc caaacagcag tgcggaagcg agcgccccca ccgaggtcca cttgccgaaa
     2581 atcaaagccg ccaacgcaat gaagccgcgc ccggtcgtca tcagcttctg gaaacggccc
     2641 acactctgca ggaccaggaa agcgcccgcc atcccggcca gcacgccgcc taaggtgaca
     2701 ttgatgtagc gggtgcgcag cacattgatc cccaccgtgt cggccgcccg cggatgttcg
     2761 ccgaccgccc gggtgcgcag tccccacggg gtgttgaaaa gtgcgaactg cagcacggcc
     2821 accaagatca acatgacata gaccagcggt tggtgggaga agaagatcgg ccccaggatc
     2881 gggatcttgg acaggatcgg gatctcgagc accgggaaga tccccacccc agaaatgtga
     2941 ttcggatcga tgtaggcgtt ggcggtgaaa ttcgtgattc cgaccgccaa gagattgatg
     3001 acggttccgc tgatgatctg gtccaccttg tagtggatcg acagccgggc atgcagcaag
     3061 gcgatcaggc ccgccgacag catcgccacc agcagaccga ccagcaggct gatcagctgc
     3121 ggcagctcac caccggtcag gtcattgaac agaaccgagc ccaagaacga catcatcgca
     3181 cccatcagca tcatcccttc aatgccgatg ttgatgacgc ccgatcgctc gcacatcagt
     3241 ccgcacatgg cccccagggc aagcggggtc gccttgacca gtgccaggga gagcaatcca
     3301 ataaagaaga cggagtccat ggcggctagt cccttcctgc ccagagctcg ctaagcctaa
     3361 ccggcccttc ttcggcccgc actcgaatcc aatagattgc gcggataatc tgattggccg
     3421 cgacgaaggt caggatcagc ccttgaacga tcgtgatgat gtcgatgggg actttggttc
     3481 gcagctgcat cagccgcgct ccggcatcca tggccccgaa taggatggcg gccagcgtga
     3541 ccccgaaggg atggatgctc ccgagcaccg ccaccgcgat gctgtcgaat ccgtatccca
     3601 cgttgaatcc ggtcgtgaaa ttatagggcg ccaaacccag cacctggatg gcacccgcca
     3661 atccgcccag ggcaccgctg agcgccatcg tgcgcacgat gttgagctcg acgctgattc
     3721 cggcatagcg ggccgcgctc gggttggcgc cgaccgtccg gatctcgaat ccgatcacgg
     3781 tccgccacaa gagccaatag accgcgatgg ccgcgagcag ggccagaatg attccggcat
     3841 gcaggcgcga atcggggatc agcagcggca gccgggcagt ctcaaagacc gcccgagttt
     3901 cgggcaccac gctccgcggg gcccgcatgg ggccatccac tccgatggcc cagttgatca
     3961 ctcgcagcgc gatgaagttg gtcatgatgg tggtgatcac ttcgtgggcc ccggtacgcg
     4021 ccttcaggta gcccgggatc gccgcccaga ttgctccgcc cagcatgcct gccagcaggg
     4081 cgacgatcaa gtgaatgacc ggcggcagct ccacgccgta gccgaccaag acggcagcca
     4141 ccgatccaac gaagagctga ccctccattc cgatgttgaa taacccaccc cggaaagcga
     4201 gggcgatgcc cagcccggcc aggatgaagg gggtcgcctt gatcagcgtc tcaacaacgg
     4261 cgacctgagt gccaaaggca ccctcggcga gtgccagata ggcctcgatc gggttggtgc
     4321 cggagacaat catcaccagg ctcgatacga gcaaggcggt gaagattgcc agaactggaa
     4381 tcgaaatccg gcccaggatc agaccgatca gattgcgcga gcgtggagca gcctcaattg
     4441 cttcagtcat ctccggcctc accaaatcga tccggcgtcc aaatggagag ccctacagat
     4501 agcgacaggg gaagcggccc ctgttgagta ccgcttcccc tgttcttacc cgttcgttcg
     4561 atgcttattc tgcgcggaag gagcctacgg cgccgcgacc ggcgtgcaga aagttcctgc
     4621 gttcgagcct tcaaagggcg cacaaggatc gatggctcca ctgtcgatca tcgcggaaat
     4681 ctcggccagc tggtccttca catcttgcgg gatcacatcc tcgaagttgt ggaagggtgc
     4741 caacacggcc ggtccgaaca cttccccccc ggggaaggtt ccttcatcga ccgagatgat
     4801 cagtgcagcc acgtccgaag tgagcgcctt cgtggcgctg gacatgatgc agtccgccac
     4861 ttcaggcagc gtgatgaact gatccacgtc caccccaatg actttgacgc cctggttgca
     4921 ggccccgatc aaggcaccgt tgcccgtcag gccgccggca ccgaacatca cgtccgcacc
     4981 ctcggtgatc agggtggcgg ccgtctcagc cgcgaactca ggatcgacga aacagacgtc
     5041 gagcgagcct gggtggtaga cgttgagcac ggtcacgtcc ggattgacgt agtgggctcc
     5101 gttgtagtag ccgacctcaa aacgggcgac cggcgggatg aacggacagc cgtacacccc
     5161 ggcgaccacg ttgctctcgg tcatcaaacc agccagcgcg ccggccagga agccggacac
     5221 gtcctcgtgg aagatcagac cggtcacgtt ggtgagcggt tccggagcaa agttcgcgtc
     5281 gacctggttc tggtcggtcc cgatgaagaa gacatcggga tggtccttgc ctgcctggtg
     5341 agtggccgcg cccatcgcga agcccgaggt gacgatgatg tcaaaaccct ctgcgatgaa
     5401 ggactccatg ttgggggtgt aatcagcgct gtcggcggtt tcgatgaatc cgtagcattc
     5461 ctcgccgagt ccgagctgct cggctgcgct caccacaccg gcataacccg cctcattgaa
     5521 gctctggtcg ttcagcttgc cgacatcggt cacgaagccg atcttgtggg cgcattcggc
     5581 tggcggaggc ggtggaactg ccgtcggcgg ctccgcggtc ggctcttccg ctgcgggagg
     5641 cggtgctact acctcctccg tcggagtagc cgtcgcacag gccccgagca caaaggccat
     5701 cgctgttaac aagaatagga tggcgtaggt ctttttcata ggtttctctc ctccaagatt
     5761 tttaatctgt tcggttctct tcactgcgcc gttgggcgcg caaattcctc gctgagtgct
     5821 cctgaaacct cgctccttcc tgcctgtagt gggcgcactg ggagactttt ccggagaggg
     5881 ccggaacccc cgaaaatcat agcatgatct cagaggtctg acaatgaatc ctcggctaac
     5941 cctcttgacc ggtggccgga tcgattccga gatcgagttg cccaccggca accgcctcgt
     6001 aggcctcctg cagtaggcgc tgccggccgg ggctgatggc ctccggattg acctccccga
     6061 gccggatccc cccgttctca atcgcatagg gccaggctgc tccgaattgc tcggcctgca
     6121 gggcgtctaa ggctttcagg atcaggccgt ccgctgcgaa caccacctgc cccgcgactc
     6181 tgaggtccgg ggtcggcaga tctccgaccc ataccagcca caggccgcct tctgccagtt
     6241 gggccccggc ctcggctgcg gctggccctg ggatgatgaa aacagcctcc acgccctgag
     6301 ctcggaagcc gtcgacagtc gcctccgaag caggggacgt gaccagtcgg cacttcgggc
     6361 aaccgtatcg cagacccgct tcgaaaccac cgcggtaaac cggctccagc ggtccccccg
     6421 tactctcgac cagccccacc catcccgtct ggccggccaa cccgaccatg actccggcca
     6481 agaacccggc ttgatcgtgc cttccgcccg gttcgcccac cgtgctcaac cgcggacccg
     6541 gctcaatctg gcccgcctct accagcacca gcggagcgac gtcggccgca atctgggttg
     6601 cccgccctcc aaagtgctct ccaaagctga cgatcgcctg cagatcgacc cgggcctcgc
     6661 ctacgattgc actggcaccc tccggatcgg aggtcagcac g
//
LOCUS       DATDPQ010000125         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3718706, whole genome shotgun sequence.
ACCESSION   DATDPQ010000125 DATDPQ010000000
VERSION     DATDPQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3718706"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VI520_03000"
     CDS             complement(<1..1421)
                     /locus_tag="VI520_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36884.1"
                     /translation="MTCSKLMQRSFAAKRASPWLLVLGYFGLATVVMTWPAVTSIGTA
                     VVGGLGDNLHFAWLIAWFDQAILSLHRLPFFAPHLNYPEGWHLARSEITPVQVLQGLP
                     FTRLGGPILAFNAVALLSFVFSGLSAYVCTLKLSGQRIAALIAGTLFAFSPFRIAHWR
                     VGQLNVLGTLWFPIYFVALADVLRGKGSGRWAGAIVGLALGLISLTSQYYLYMTLVAS
                     ALGSAIYLLGFRRDRLGNSSLRRSLATMAMVAAPLIAIGLFPYIELALQDSYPSRSLY
                     DVSSGSASVTDFLLPSTDHFLWGGWVGENFSRDHWPEATLYFGVITTGLALLAVLKAW
                     RAGSGVRVLVVFAGVSILLSLGVHLYWNEQLVLVRLPEAIASILHRDETRVPMLGYLL
                     HKFVPFYSTMRTFKRAGALALTAIALLAGIGAAELLNRLKSSWRMPAGVLLLAMVWLD
                     FYPGPFKEFSKVEPRAVDQWLAT"
     gene            complement(1418..3040)
                     /locus_tag="VI520_03005"
     CDS             complement(1418..3040)
                     /locus_tag="VI520_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HLF36885.1"
                     /translation="MIGVTRPFRQPFWFWLLLAVLASGSVLYLTRLGPGAGGDSTSYL
                     MGAENLLLGNGFSRYSGGYEIRPITGFPPVYSVALAAAGLTGLELVEAARLLNAVLFG
                     ANVGLTSAIVYSLTRSTWGSILAGLLFMARTMLLELHAWVMSEPLFIFLSLVSACLLG
                     RYLNDRGMGWLIAAGTVTALACLTRYVGAALVGTGLVGIALFGPPDLRRRGRDGLIFA
                     GLSLIPLLMWLARNRAAAGTAVNRGLSYHPMEPELVRLFLADISSWFVPHQVPLPTGV
                     RATLAIGIAAAVLGGFVILLGRHWLKWDRTGLLMVGPAGTRLAAVPWLLALYAAGGLF
                     ITWLNSTLLDAATTAAAPSRYLAPVFAASLILFVTAAVHLLRLMSGRRRPGRIAAAAG
                     LVLLAFYIYNSLGFFRDPLPRLGYTGRKLLSPELVAALAQIPAESPIVSNNPELIYIL
                     AGRPAYVRPIHYDPYQAEYRDDYELQFAQLEAQLGRGGVFVVFDELEADDLAVIERMD
                     LQLLAQYPMAQIFGHPSGPTFGGLYPPNSDLA"
     gene            complement(3122..>3397)
                     /locus_tag="VI520_03010"
     CDS             complement(3122..>3397)
                     /locus_tag="VI520_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36886.1"
                     /translation="AALIREGGCTEVGISFAGGGIEYPMWPLLGAPDPDLRVEWVVSG
                     TASEAYRDPAFRPCAVVCQHCETQASMSDLPLTYGRSEFRLYMNTED"
BASE COUNT          809 a         1039 c         1019 g          530 t
ORIGIN      
        1 tgagtggcca accattggtc aaccgctcgc ggctcgacct ttgagaactc cttgaaaggg
       61 ccagggtaga agtccagcca taccatcgcc aatagcaaga cgccagcagg cattcgccag
      121 ctggacttca agcgattcag cagctcagct gccccaattc cagcgaggag ggcaattgct
      181 gtaagggcca gcgcacctgc ccgcttgaat gtcctcatcg tgctgtagaa cggcacaaac
      241 ttatgcagca agtaaccaag catcggcacg cgggtttcgt cgcggtgaag aatcgaggcg
      301 attgcctctg gaagtctgac aagaaccagc tgctcgttcc agtagaggtg gacaccaagg
      361 ctcagcagga tcgagacacc tgcaaagacc acgagcactc gaactccgct tccagctcgc
      421 caagccttga ggacggccag caaggccagc ccggtcgtta tcacgccaaa atacaacgtc
      481 gcttcgggcc agtgatcgcg ggagaagttc tcaccaaccc agccgcccca cagaaaatga
      541 tcggtcgaag gcagcagaaa atccgtgacg ctggccgacc ctgacgagac atcgtacaag
      601 gagcggcttg gatagctgtc ttgaagcgca agttcgatat agggaaacag tccgatcgcg
      661 atcagtgggg ccgccaccat cgccattgtc gccagtgatc gcctgagcga cgagttgcca
      721 agccggtcgc gccggaatcc cagcaggtag atcgccgagc ccagtgcgga ggcaaccagg
      781 gtcatgtaaa ggtagtactg agaagtcagg cttattagtc caagcgccaa tccgacgatg
      841 gctcccgccc acctgccaga acctttgccc ctgagcacat ccgccagcgc aacgaagtag
      901 atcgggaacc atagcgtgcc gagcacgttc agctgaccca ctcgccagtg ggctatgcgg
      961 aaaggcgaga atgcaaacaa ggttcccgct atgagtgctg caatgcgttg gccggagagt
     1021 ttcagcgtgc agacataggc cgatagcccg gagaagacaa aggagagcag tgcgaccgca
     1081 ttgaaggcaa gaatcggtcc ccccaagcga gtgaaaggca atccttgcag gacctgaacc
     1141 ggtgtgatct cgcttctcgc cagatgccac ccttcggggt agttcaagtg cggcgcgaag
     1201 aacggcagcc ggtggaggga taagatcgct tggtcaaacc aagcgataag ccatgcgaag
     1261 tggagattgt cgcccaaccc accgacgacg gcggtgccga ttgaagttac ggctggccag
     1321 gtcatgacga cagtagccag cccaaagtag cccaaaacga gaagccaggg cgaagcccgc
     1381 ttagccgcga aggagcgctg catgagcttg gagcaggtca agcgagatcc gagttgggag
     1441 ggtacagacc accgaaggtt ggcccgctcg gatggccaaa gatctgggcc atcggatact
     1501 gcgccagcaa ttgcagatcc atccgctcaa tgaccgcaag gtcgtcagcc tcgagctcat
     1561 cgaataccac gaacacccct ccccgcccga gctgagcttc cagttgggcg aattgaagtt
     1621 cgtagtcgtc ccggtactcg gcctgatacg gatcgtaatg gatcggccgg acataggccg
     1681 gccggccggc caggatatag atcagctcag ggttattcga aacaatcggt gattcggcgg
     1741 gaatctgcgc cagggcggcg accagttcgg gcgagagaag cttgcgaccg gtgtagccca
     1801 gccggggaag aggatcacgg aagaagccca atgagttgta gatgtagaac gccagcagca
     1861 ccaatcccgc agcggcggca atccggcctg gccgccgccg gccgctcatg agccgcagca
     1921 ggtggacggc agcagtgacg aagaggatca aggaggcggc aaaaaccggg gcgaggtagc
     1981 gggacggggc cgcggcggtg gtcgccgcgt cgagcagggt cgaattcaac caggtgataa
     2041 acagaccccc ggcagcgtac agtgccagca gccagggtac ggccgccagt ctggttccgg
     2101 ccggaccgac catcagcagg ccagtccggt cccacttcaa ccagtgcctc ccgagcagga
     2161 tcacgaatcc tcccagcaca gcggccgcga tgccaatcgc caatgtggcc cgcacacccg
     2221 ttggaagtgg aacttgatgg ggcacgaacc aggaactgat gtcggccagg aagagccgaa
     2281 ccaactccgg ctccatcgga tggtagctca ggccgcgatt gaccgccgtt ccggcggcgg
     2341 cccggttgcg cgccaaccac atcaacagcg gaatcaggct caacccggca aagatcagcc
     2401 catctcggcc tcggcgacgc aggtcgggcg gcccaaacag ggcaattccc accaatccgg
     2461 tgcccaccag ggcagctcct acataacggg tgagacacgc cagcgcggtg accgttcccg
     2521 ccgcgatcaa ccatcccatc ccgcggtcat tcaagtagcg tccgagcagg caggccgaga
     2581 ccaacgacag aaagatgaag agcggctcgg acatcaccca agcgtgaagt tccaacagca
     2641 tcgttcgggc cataaagagc aggccggcga gtatggagcc ccaggtcgac cgggtcaggc
     2701 tgtagacgat ggccgaagtc agcccgacgt tcgcaccgaa aaggacagca ttcagcaggc
     2761 gggccgcctc aaccagctcc agtccggtca ggcctgccgc cgcaagggca accgagtaaa
     2821 ccggcggaaa accggtaatg ggtctgatct cgtagccgcc ggagtaacgg ctgaagccat
     2881 tacccaggag caggttttcc gcgcccatca ggtaagaagt ggaatctccg cctgcgccgg
     2941 gtcccagccg ggtcaggtac agcacgctcc cgctcgcgag caccgcgagt agcagccaga
     3001 accagaatgg ttgcctgaat ggtctagtta cgccgatcat tgttcgtgtc tgaccaatct
     3061 gtaggagacc ggctaatcat aacggcctcc tcccgctcga tcatattttg gactggagga
     3121 ttcaatcctc ggtgttcata tacagccgga attcgctccg accataggtc aagggaaggt
     3181 ctgacatgct ggcttgcgtc tcgcagtgtt ggcagaccac tgcacagggc cggaaggccg
     3241 gatcccgata cgcttccgaa gccgtcccgg acactaccca ttccactctc aggtcaggat
     3301 ccggtgcgcc caatagcggc cacatcggat actctatccc gcctccggca aacgagatgc
     3361 caacctccgt gcatccacct tcccgaatga gagctgc
//
LOCUS       DATDPQ010000126         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2151881, whole genome shotgun sequence.
ACCESSION   DATDPQ010000126 DATDPQ010000000
VERSION     DATDPQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151881"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VI520_03015"
     CDS             <1..793
                     /locus_tag="VI520_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLF36887.1"
                     /translation="EEGGSIEQVEKAMIYSIFQLDDTLAREVMVPRIDMLAFEEQTTL
                     SEATDRLLETGYSRAPVYRGTIDDVIGLVFVKDLLGAWQEGEHGRTVGQLKREAYFVP
                     EAKKVDELLEELQSRRVHMAVVVDEYGGTAGLVTIEDIVEEIIGEIRDEYDVAEEASF
                     HQLEDGEYVFSGGIDLDDVNEIAGAELPKQAGETLGGFIYSELGRVPSPGDRLESGGL
                     ELVVDQVVRRRIRRVRARKLTAEPAVEARDNGQGRVDGAERRGNR"
     gene            765..1175
                     /locus_tag="VI520_03020"
     CDS             765..1175
                     /locus_tag="VI520_03020"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HLF36888.1"
                     /translation="MEPSEEGIAELIRRAQAVRRRAYAPYSNYPVGAALLGRSGQIYE
                     GANVENAAYPNTMCAERNAVFRAVSDGERAFDAIAVVTENGGAPCGACRQVLSEFGGE
                     VLVIIADTTGKIHHKTPLHELIPHAFGPQDLVLS"
     gene            1218..2000
                     /gene="recO"
                     /locus_tag="VI520_03025"
     CDS             1218..2000
                     /gene="recO"
                     /locus_tag="VI520_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLF36889.1"
                     /translation="MTERARVYRTDAIVLRRVDLGEADRVLTLFTLQHGKLRAVAKGA
                     RRPGSRKAGHLEPFTLVNLLLARGRELDIITQAEAVRTFPQVRADLLRLSLAAYVAEL
                     TDRFGIQESDSHGLFQLAADTLDRLDREAVPDVALRYFQVRLLDLAGFRPELFRCVSC
                     SNAAQPVDQFFSLSEGGLLCPNCGPQREEALPLSLSALKILRHTQRSSYEQAARPKLS
                     PGVAQEVDGLLESYLSYVLERKLNVPGFVRQVRRLKEARVYS"
BASE COUNT          407 a          646 c          739 g          403 t
ORIGIN      
        1 ggaagaaggc ggttccatcg agcaagttga aaaggcgatg atctattcca tctttcaact
       61 ggatgacacc ctggcccgtg aggtgatggt tccccggatc gacatgctcg cctttgagga
      121 gcagaccact ctgtccgagg cgaccgaccg gctgttggaa accggctact ctcgggcgcc
      181 ggtctaccgg ggtacgatcg atgatgtaat tgggttggtc ttcgtaaagg atctgctcgg
      241 cgcctggcag gaaggggagc atggccggac ggtcggtcag ctcaagcggg aggcttactt
      301 cgtgccggag gccaagaaag tcgatgagct gcttgaagaa ctgcaatcgc ggcgggttca
      361 catggcggtc gtggtggacg agtacggcgg caccgccggt ctggtcacga tcgaggatat
      421 cgtagaggag atcatcggcg agatccggga cgagtatgat gttgccgaag aggccagctt
      481 ccaccagttg gaggacggcg agtatgtctt cagcggaggg atcgatctag acgacgtcaa
      541 tgagatcgcc ggagcagaat tgcccaaaca ggccggggag accttggggg ggttcatcta
      601 tagcgagctt gggcgagttc cctctccggg tgacagactc gaatcaggtg gtctggaact
      661 ggtcgtggat caggtagtcc ggcgtcgaat ccggcgggtg agggcgcgga agctgacggc
      721 cgaaccggca gtagaggccc gcgacaacgg gcagggtagg gtagatggag ccgagcgaag
      781 agggaatcgc tgaactgatc cgccgggccc aggcggtccg ccgcagagcc tacgccccct
      841 attcgaacta cccggtaggg gccgccttgc tgggccgctc gggtcagatc tatgaggggg
      901 cgaacgtcga gaacgccgcc tatcccaaca cgatgtgcgc cgagcggaac gcggttttcc
      961 gagcagtgtc ggatggggag cgggctttcg atgcgatcgc ggtggtcacc gaaaacggcg
     1021 gcgctccctg cggggcctgc cggcaggtgc tgtccgagtt cggcggcgag gtgctggtca
     1081 tcattgccga cactaccgga aagattcacc acaagacccc gctccacgaa ctcatccccc
     1141 acgccttcgg gccccaagac ctggttctct cctagtcccg ataggactca attaccccga
     1201 tgggtataat cagccgggtg accgagcgag ctcgagttta tcgcacggac gcgatcgtgc
     1261 ttcgccgggt agatttgggc gaggcggatc gagtgctgac cctgttcacc ctccagcatg
     1321 ggaagctgcg ggcggtggcg aagggggcgc gccgtccagg atccaggaag gccggtcacc
     1381 tcgagccatt caccctcgtc aatctgctgc tggcccgcgg gcgcgagctg gacatcatca
     1441 cccaagccga ggccgtgcgg acttttcccc aagttcgggc agacctgctc aggctctcgc
     1501 tggccgccta cgtcgcggag ctgaccgatc ggtttgggat tcaagaaagc gactcgcacg
     1561 ggcttttcca gctcgcggca gacaccttgg accggctgga tcgagaagcg gtgccggacg
     1621 ttgcgctgcg ctactttcag gtccgcctat tggacctggc gggctttcgg ccggagctgt
     1681 tccgctgcgt cagctgctcc aacgcggcgc agccggtgga tcaatttttc tcgctttctg
     1741 aaggtggcct gctttgcccg aactgtggcc cccagcggga ggaggccctg cctttgtcgc
     1801 tctcggcact gaaaatcctt cgccataccc agcgcagttc ctatgagcag gccgcccggc
     1861 caaagctcag cccaggcgtg gcgcaggagg tagatggatt gctggagagc tatctcagct
     1921 acgtactcga acggaaactg aacgtgcctg gcttcgtgcg ccaggtgcga cggctgaagg
     1981 aggcacgcgt ctattcctag ccgatcgccg ggcaggcgaa agcggaaacc ctcctcgggg
     2041 tctcggccac cggccaccac ccccgggctg acattccagc aggcggtctc cgcactcgag
     2101 cggttctggg cgaatgaggg ctgcctgatc tggcagccct attacaccca ggtcggagcc
     2161 gggacgatga acccggcgac cttcctgcgg gtctt
//
LOCUS       DATDPQ010000127         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_570928, whole genome shotgun sequence.
ACCESSION   DATDPQ010000127 DATDPQ010000000
VERSION     DATDPQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570928"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VI520_03030"
     CDS             complement(<1..1314)
                     /locus_tag="VI520_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF36890.1"
                     /translation="MTRKILLAVLILLGVVLVMAPNLIAELPDRDAGVFLYVGSRILD
                     GEIPYRDIWDHKGPLIYYINAIGIALTPGSERGVWALEVITLSLSGFGLYRLLRKGFG
                     PLPAVLAVGLFYAGLGYVLYPGNYTEEFSLPLQIGALLLFDRTEAAGGRPWRWAAIGL
                     TGAATVLLRPNNAGIHLAIFIYVILESLSRGGFRRGLANSAYLGVGVLLLLIPVLAYF
                     GWQRALPELFDSVIRFNSAYVEATVKARIDSILEGFRLLAPTGLPVFTLAVWTMACLR
                     LVVPGSRSQEPRLVRLAVIGFPIELLMVGISGRSLNHYFIAWLPISAVLAARFFQHLM
                     GPSDESELRLGAAISPRVGWAVGLALVILILPLRRLLPPFLTFLNNGSRDAFERAADL
                     TQYDEEYLLMWGAESTFNFLSDKPAPTRYVYQYPLYTCGYVSPSMV"
     gene            complement(1352..>2572)
                     /locus_tag="VI520_03035"
     CDS             complement(1352..>2572)
                     /locus_tag="VI520_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36891.1"
                     /translation="GLGVLTKVTFFAYGAPLMGGFLLRRLRQLGFRRTLTTSIVLAAL
                     VSGLNLPTWFRNLTTYGGLYGSPDWSLSMSAFPDFVEAVSRVRESLSAAPTANSANGR
                     SEPLSAARDVVLWPLSRIGQAAALNLVTPSHLVNQWLWSALDAVPGIFGGVVSNSLQP
                     AAWSHEDSAGNPLHLLLVLVSAVFIARSARKLRPVTWTVAYASMIALGYLLLPIVISP
                     ASSDFGIRFQLTFFILWAPLIGPAMPPIRTSGWMTIIGILLLISALPYLLFNNTRPVI
                     GLPPWPTRVHSVFVAPSAEIMFAPNPEKRAAYEHLAESVENSGCQEIGMRLDSSDLEY
                     QFWWLLKAPQSGYRLETIYTYPELEPLLDRSFDPCGVICTVCGERTQLGGLVMKSDFG
                     GARLFLEDDAGKNQ"
BASE COUNT          593 a          752 c          791 g          436 t
ORIGIN      
        1 aaccatgctc gggctcacgt aaccacaggt gtagagggga tactggtaga catagcgagt
       61 gggcgcgggc ttgtcggaga gaaagttgaa cgtgctttca gccccccaca tcagcaagta
      121 ttcttcatca tactgggtca agtcagccgc ccgctcgaag gcatcgcggc tcccgttatt
      181 taggaatgtc agaaatggcg ggagcagccg gcgcaggggg agaatcagga tcacgagtgc
      241 caacccgacc gcccaaccaa cccgggggct gatggccgcc ccaagtctga gctctgattc
      301 gtcagagggt cccatgaggt gctggaagaa ccgcgcggcg agcaccgccg agatcgggag
      361 ccaggcaatg aagtagtgat tgaggctccg tcctgagatc cccaccatga ggagctcgat
      421 cgggaaccca atcactgcaa ggcgcaccag ccgcggttcc tgagagcgac tgccgggcac
      481 cacgagccgg aggcaggcca tggtccacac cgcgagggta aagaccggca acccggtggg
      541 agctaggagt ctgaagcctt cgaggatgga atcgatcctg gctttcacag tcgcctcgac
      601 ataggcggag ttgaacctga tgacggagtc aaacagctcc ggaagcgcac gctgccaacc
      661 aaagtaggcc aggaccggaa tcagcaacag cagtacaccc acgcccaggt aggctgagtt
      721 cgccagacca cggcggaacc cgccacggct caaggactct aggattacat agatgaagat
      781 ggccaagtgg atccctgcgt tgttgggtcg caggagaacc gtggctgccc cagtcaggcc
      841 aatcgccgcc caccgccatg gcctgccgcc cgcggcttcc gttcggtcga aaagcaacaa
      901 agctccgatc tgcagcggaa gcgagaattc ctcggtgtaa ttgccaggat aaagaacgta
      961 gccgaggccg gcgtagaaga gcccgaccgc gagcaccgcc ggcagcggac cgaacccctt
     1021 gcgcagcagt cggtacaacc cgaatccgga gagactgagc gtgatcactt caagcgccca
     1081 gactccccgc tcggatccag gggtcaaggc gatcccgatg gcattgatgt aatagatcag
     1141 tggccccttg tggtcccaga tgtccctata gggaatctct ccatccaaga tccgcgatcc
     1201 gacatagaga aagacgcccg cgtctcggtc cggcagctcg gctattagat tcggtgccat
     1261 taccaacaca acaccgagca agatcaggac cgctagcagg attttccgag tcatcgatcc
     1321 gatcgcgcgg tttctcttga tgccgaaggt cctattggtt cttgcctgca tcgtcctcaa
     1381 ggaagagtcg agcgcccccg aagtcagact tcatgaccag accgccaagt tgggttcgct
     1441 cgccacagac ggtgcagatg accccacagg ggtcgaagct ccggtcgagc aggggttcca
     1501 gctctggata ggtatatatc gtctccagac gatagccgct ttggggggct ttcagcagcc
     1561 accagaactg gtactccaaa tcgctcgaat ccagccgcat gccaatttcc tggcatcccg
     1621 agttctccac cgactcggcg agatgctcgt aggctgctcg cttctcaggg ttcggtgcaa
     1681 acatgatctc cgctgatgga gcaacaaaca cactgtgcac gcgagttggc cagggcggga
     1741 gcccgatgac cggccgggtg ttattgaaca gcaaataggg tagggctgat atcagaagaa
     1801 gaatccctat gatggtcatc caacccgagg tccgaatcgg cggcatggca ggcccgatca
     1861 gcggggccca gagtatgaag aacgtaagct gaaatcggat gccgaagtcc gagctcgcgg
     1921 gacttatcac gatcgggaga agcaggtagc cgagcgcgat catgctcgca tacgcgactg
     1981 tccaggtgac aggacggagt ttgcgggcgc ttcgcgcgat gaagaccgct gaaaccaaca
     2041 ccaacaagag gtgcaacggg ttgccggccg agtcctcgtg gctccaggct gccggttgga
     2101 gcgagttcga gacgacgccg ccaaagatgc cgggaaccgc gtccagggca ctccacaacc
     2161 attgattcac caggtgggag ggggtgacca gattgagggc cgctgcctgg ccaatcctgc
     2221 tcagcggcca caacacgacg tcccgagccg cactgagtgg ctcggagcgc ccgttcgcgg
     2281 agtttgcggt aggggcggca gagagcgatt ccctgactcg gctgactgct tccacgaaat
     2341 cgggaaatgc cgacatggat agggaccagt ccgggcttcc atagaggccg ccgtaggtgg
     2401 tgaggtttcg gaaccaggtt ggcagattca gcccacttac caaggcagct agcacaatag
     2461 atgttgtcag cgtccggcga aatccgagtt gccgaagtct cctgagcagg aatccgccca
     2521 tgagcggggc accatatgcg aagaaggtga ctttggtgag gactcctagc cc
//
LOCUS       DATDPQ010000128         6036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3446806, whole genome shotgun sequence.
ACCESSION   DATDPQ010000128 DATDPQ010000000
VERSION     DATDPQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3446806"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VI520_03040"
     CDS             <1..684
                     /locus_tag="VI520_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLF36892.1"
                     /translation="MLLVDHEGLADIAIRAAQAGLATSVHAIGDQATHEVLGAFSTLR
                     EYEAANQLPHLRHRIEHLQLLHPDDSGRAGALNLVASMQPVHATSDMPMADRYWGDRV
                     RTAYAWRTELQAGAHLAFGSDAPVEDPNPFLGIHAAVTRRRLDGSPGPQGWVPNQRVT
                     LEQAMAAYTLGPAYAAGMESVQGRLEPGYLADLVVLARDPYACRPDELAGLAPVGTMV
                     GGEWRYRDF"
     gene            complement(681..2573)
                     /gene="selB"
                     /locus_tag="VI520_03045"
     CDS             complement(681..2573)
                     /gene="selB"
                     /locus_tag="VI520_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HLF36893.1"
                     /translation="MRVLGTAGHVDHGKSALVEALTGIDPDRLREEKDRQMTIDLGFA
                     WMTLPSGEGLGIIDVPGHLDFIDNMLAGVGGIDAALLVVAADEGPMPQTREHLAILDL
                     LAIPHLIVVLTKIDLIDDPEWLELVRREIRSLLAGGRFAQAEILPASSVSRVGLPELI
                     RLLDEMLADEPVRADARHPRLPVDRVFTMAGFGTVVTGTLLDGPFQVGDEVELLPGGF
                     GARLRGLQTHKQAVTIAHPGSRVAANLSGVEVDQIQRGDVLVLPGTDAPTSVVDVSFR
                     VLPDEAFAIRHNSSAKFFAGSANRMARIRVLSEQPLQSGEAGWLQLVLEAPVVVRRGD
                     RFILRRPSPGTTLGGGQVANPHPTQFHKRVDPSVIEGLNRALSEDPEDVLLAYIGEEG
                     PSRVESVRESLGMDLVAADAALDRLEQKGKLVIFNGAAPSTERWAIGAQRWSATAKRA
                     RELVGEYHRRHPLRSGMSLEELRGQLAGAATELIGELVNRGMLRQAGARVSLPEFVVR
                     LSPTEVEQVSRLLEQFAAEPYGPPGWNVCRETVGEEVLASLLENGELVRVSETVLLSR
                     GAYSQMTDWVRERLGETGKVTVSEVRDRFASSRKYALALLEHLDQIGVTARDGDFHRQ
                     SPAVGN"
     gene            complement(2599..3840)
                     /locus_tag="VI520_03050"
     CDS             complement(2599..3840)
                     /locus_tag="VI520_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36894.1"
                     /translation="MLYSADSRILAKIPGPTSQESQLETEQIDKRFTWLDEQRRRDGE
                     EIGRVAERLEGLEQELRRLADQIQSLAGDQSRTAAVATKASQMDETISQHRLEINGLI
                     EGAEQRRIARERQLEDLRKSGERQTAEALDAVRVELKATKDLRDSLEARRQEELRISR
                     TLDNLSKRIESLGREFEERNRQTSSLQEGRRQDAQRIAELESRAAEQHKKHESQVVHL
                     QVAEDQARRVQAKLGELEASESELRQAQTLWTEQQAVRQAEFERGWKAQEKRFNVFAG
                     QAEGLDERLAAFEETHRSLKRLESELSDVVARLERRIEEVSEIQRLSEDRLKQEWSGQ
                     QADEHKRWSAFKLEAEEQRRDHERLHQKLSDELRSTQEDLGEALEGLEQLSHSGQQRL
                     QETLTMLREWAAEATGKESQP"
     gene            complement(3867..5255)
                     /locus_tag="VI520_03055"
     CDS             complement(3867..5255)
                     /locus_tag="VI520_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLF36895.1"
                     /translation="MSAPAVIFGLTAILSGGSLMLRRRIWAPFALAALGSLMIGVFAL
                     TAPIESPGSLFGMSFKFEGGWRILGRLIALSNRNRAPVAFLYLTGALFFGAGMIARPG
                     RSFAPVGLITLGLLAGSLAIEPFLYAAIFLELAAMAAILVLNPPDLRADPGSLQLLVL
                     YSLAMIAILFTGWLLDNVGITSVTPDLASKVMTLLALGFSILMFVPPFHFWLGRAAET
                     ANPFSLGFIAVVLQSAGLFFLLRFLDSLEWLRTEPEVFTAALSVGLLMVGLGSLAAAA
                     QASYSKMVAYALIADFGVTLFAIGSNQPGALHIVLLHVGVRVVSLAAWGLGAAVMGRT
                     ASDGHLIGAGRVNPIAASASLIGALGLAGFPLTGGFVIRWALLRSLPETADAGVLILL
                     CSMLLVTLAVGRWAAQFFSSAGERETGLVRGTNRWILLAGILLCLLLGGFPQLGMPWV
                     APALRGFADLQH"
     gene            complement(5252..5833)
                     /locus_tag="VI520_03060"
     CDS             complement(5252..5833)
                     /locus_tag="VI520_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36896.1"
                     /translation="MTALIVSGTALLLTVFTGRVARIGLLAIQYAAVAWLTGLSLPLQ
                     VAAVKLVAGMIACAILAVSESKQQQAPAQPAGREFGTTFRLFASGLGLVAAFGLGQRN
                     WFEVPDLRQEAILGSTVLLTMGLLQLGLSGSAFRVCLGLMTLLSGFEVVYGLIEPSLA
                     VLALIASIHIGLALVASYLGLISGAPEEVQGAP"
     gene            complement(5830..>6036)
                     /locus_tag="VI520_03065"
     CDS             complement(5830..>6036)
                     /locus_tag="VI520_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36897.1"
                     /translation="RILPRRSSAGGRWPLALRIDIRDDLSRAWELVARWIRSGTELLE
                     GQSAVLWMFLAVLAIVIAIGGSPG"
BASE COUNT         1161 a         2032 c         1756 g         1087 t
ORIGIN      
        1 atgttgttag tcgaccacga ggggctggcc gacatcgcta ttcgtgcggc ccaagctggg
       61 ttggcgacca gcgtgcatgc tatcggagac caggcgaccc acgaagtgct gggggcattc
      121 agcacactgc gggaatatga ggccgccaac cagcttcccc acctgcgaca ccggatcgag
      181 cacctgcaac tgctgcatcc agacgattcc ggcagggccg gcgctctcaa tctagtggcc
      241 tcgatgcagc cggtccacgc aacctcagac atgcccatgg ctgatcgata ctggggagac
      301 cgggtgcgga ccgcgtatgc ctggcggacc gagttgcagg cgggcgctca cttggccttc
      361 ggatcagacg ccccagtcga ggatcccaat cccttcctag ggatccatgc cgcggtcacc
      421 cggcgccgac tggacggcag cccaggcccg cagggatggg taccgaacca acgagtgacc
      481 ctggagcagg ccatggctgc ctacacactg ggacctgcct atgcggccgg gatggaatcg
      541 gttcagggac ggctggagcc cggctatctg gcggacctgg tcgtgctggc tcgcgacccc
      601 tacgcttgtc ggccagatga actggccgga cttgccccgg tcggcacgat ggtcggaggc
      661 gagtggcgct accgcgattt ctagttgccg accgccggac tttggcgatg gaaatcgcca
      721 tcccgcgcgg tgactccgat ctgatccaaa tgctccagga gagccagggc gtatttgcga
      781 gaactcgcaa accggtccct gacttcgctg acggtcactt tcccggtctc gcccagtcgc
      841 tcccgcaccc aatcggtcat ctgtgagtac gccccccggc tcaagagcac ggtctctgag
      901 accctcacca gctcgccatt ctcgagcagc gaggcgagta cttcctctcc gacagtttcc
      961 cggcacacgt tccagccggg cggcccatac ggctcggccg cgaattgctc cagcagccga
     1021 ctcacctgct cgacttcggt cgggctgagt cggaccacga attccggaag gctgactcgg
     1081 gcgccggcct gccgcagcat cccgcggttg accagctccc cgatcaattc agtggccgct
     1141 cccgcgagct ggcccctgag ctcctcaaga gacatccccg atctcaatgg gtggcgccgg
     1201 tgatattccc cgaccagttc tcgcgcccgc ttagccgttg cgctccacct ttgggccccg
     1261 atcgcccacc gctcggttga aggcgctgcc ccgttgaaga ttaccaactt gcccttctgc
     1321 tcgagccggt cgagcgccgc gtcggcggct accaaatcca tccccagaga ctctctcacg
     1381 gactcgaccc gcgatggccc ctcctcgcca atgtacgcaa gcaagacgtc ttctgggtcc
     1441 tcgcttaggg ctctgttcaa tccttcgatt acgcttggat ccacccgctt gtgaaattga
     1501 gtcgggtgag gattcgcaac ctgtccaccc ccgagggtcg tccccggaga cgggcgacgc
     1561 aggatgaacc ggtctcccct tctcacgacg acgggggctt ctagcaccaa ctgcagccac
     1621 ccggcttcgc ccgattgaag cggctgctcg ctcaggactc gaattcgggc catccgattc
     1681 gcagaaccgg cgaagaactt tgcgcttgag ttatgccgaa tggcgaacgc ctcgtccgga
     1741 agtacccgga aactcacgtc gaccacgctg gtcggcgcgt ccgttccagg gagaaccaga
     1801 acgtctcccc gttgaatctg gtcgacctcc acacccgaga gatttgccgc gacccggctt
     1861 ccggggtgag caatggttac cgcctgcttg tgggtctgca ggcctctgag ccgcgccccg
     1921 aacccgccgg ggagcagttc cacctcatcg ccgacctgga agggcccatc caggagcgtc
     1981 cccgtgacga cagtcccgaa gcccgccata gtgaacactc gatccaccgg taaacgtgga
     2041 tgcctggcgt ctgcccttac cggttcgtcc gctagcatct cgtctagcag cctgattagc
     2101 tcgggaagcc cgacccgaga caccgatgac gcaggcagaa tctcagcctg cgcgaatcgg
     2161 ccacctgcca gcaggctgcg gatctcgcgc cggaccagct ccaaccactc ggggtcgtca
     2221 atgagatcga tttttgtcag gacgacaatg agatgtggga ttgccagcag gtccaggatc
     2281 gccaagtgct cgcgggtctg cggcatcggc ccttcatcgg ccgcaactac caatagcgcg
     2341 gcgtctatgc ctccgacccc ggccagcatg ttgtcaataa aatcaagatg gccagggacg
     2401 tcgatgattc caaggccttc tccactcggc agggtcatcc aggcaaagcc cagatcgatc
     2461 gtcatctgcc ggtccttctc ttctcgtagg cgatccggat ctatcccggt cagtgcctct
     2521 accagggcgg acttgccgtg gtcaacatgg ccggcagtcc ccaaaacccg catagctcga
     2581 gtactctacg ccctcaaatc agggctggct ttcctttcct gtggcctcgg ccgcccattc
     2641 ccggagcatc gttagggtct cctgcaggcg ctgctggccc gagtggctga gctgctcgag
     2701 gccttccagg gcctcaccga ggtcttcctg ggtcgaccgc agctcgtcag acaacttctg
     2761 atgcagtcgt tcatgatcac gccgctgctc ttcggcttcc agcttgaagg cactccaacg
     2821 cttgtgttca tccgcctgtt gacccgacca ctcttgcttg agccgatctt cactcaaccg
     2881 ctggatttcg ctgacctctt cgatccgccg ttccaggcga gccacgacgt cgcttaactc
     2941 actttccagc cgcttaaggc ttcggtgggt ctcctcgaag gccgccagcc gctcgtcaag
     3001 tccttccgct tggccggcga aaacgttgaa tcgcttctcc tgcgctttcc agccgcgctc
     3061 gaattcggcc tgacggaccg cctgctgctc ggtccacagc gtttgggctt gacgaagctc
     3121 gctctcgctg gcctccaatt cgccgagctt tgcctgcact cggcgcgcct gatcctcggc
     3181 aacctgcagg tgaaccacct gagattcgtg cttcttgtgt tgttcagccg cccgggactc
     3241 caattcggcg atccgttggg cgtcttgtcg cctgccttcc tgcaggctgg aagtctgccg
     3301 atttcgctct tcgaattccc tgccaaggga ctcgatccgt ttcgacagat tgtccagcgt
     3361 acggctgatc ctcagttctt cttggcgacg cgcctccagc gaatcgcgca ggtctttggt
     3421 ggccttcagc tcaacccgaa cggcatcgag cgcctcggcg gtctggcgct cccccgactt
     3481 gcgcaaatcc tcgagttgtc gttcgcgggc gatccggcgt tgctctgctc cctcgatcag
     3541 cccattgatc tccaaccgat gttggctgat cgtttcgtcc atctggctgg cctttgttgc
     3601 cactgcagcg gtccgggatt gatctccagc caggctttga atctggtccg ccagccggcg
     3661 tagttcctgc tcgaggccct ccagccgctc cgctacccgg ccgatctcct cgccgtctct
     3721 tcggcgctgc tcatcaagcc aggtgaatcg cttgtcgatc tgttcggtct ccagttgaga
     3781 ctcctgggag gtgggtccgg gtattttcgc caagattcgg gagtccgctg agtatagcat
     3841 agcggtcccg ccgccttgtt tccgccttag tgctgaagat cggcaaagcc tcgaagggcc
     3901 ggtgccaccc atggcatgcc aagctggggg aacccgccga gcagcagaca cagcaggatt
     3961 cccgcgagca agatccatcg attcgtgccc ctcacaagtc cagtctccct ctcgccggcc
     4021 gaggagaaga attgggcggc ccatctccca accgccagcg taactagcag catagagcag
     4081 agcaggatta gcacccccgc atcggccgtt tcaggaaggc tgcgtaacag cgcccatcgg
     4141 atcacgaatc cgccggtcaa cgggaatcca gccaggccga gtgcgccgat cagactggcg
     4201 ctcgccgcga ttggattgac ccggcccgcg ccgatcaggt ggccgtcgct cgcagttcgg
     4261 cccattaccg cggctccgag cccccatgcc gccaggctca cgacccggac ccccacatgg
     4321 agaagcacga tgtgaagcgc cccgggttgg ttcgatccaa ttgcaaacaa ggtgactccg
     4381 aaatcggcga tcaacgcgta ggcaaccatc ttcgagtagg atgcctgggc ggcggctgcc
     4441 aacgacccca gaccgaccat cagtaaccca accgacaacg cggcggtgaa cacttcgggt
     4501 tcggtccgca gccactcgag gctgtccagg aaccgaagca ggaaaaacag accggcgctc
     4561 tgcagaacca ccgcgatgaa tcctaatgag aagggattcg cggtctcggc cgcccgcccc
     4621 agccagaagt ggaaaggcgg aacgaacatc aggatcgaaa accccagagc caagagagtc
     4681 atcactttag acgcaaggtc gggggtcacg ctggtgatac cgacgttgtc gagcagccat
     4741 ccggtaaaca ggatggcaat catcgccagg ctatagagga cgagcagctg gagggatccc
     4801 ggatcagccc tcaggtctgg aggattaagc accaggattg ccgccatggc cgcaagctcc
     4861 aggaagatgg ctgcatacag gaatggttcg atggccagcg atcccgcgag cagccccagg
     4921 gtgatcaggc caaccggggc gaaagaacgg cccgggcggg cgatcatccc ggccccgaaa
     4981 aacaacgcac ccgtcagata gagaaacgcc actggggccc ggttgcgatt cgaaagcgcg
     5041 atcagccgac cgaggatgcg ccagcctcct tcgaacttga acgacattcc gaaaagcgag
     5101 cccggggatt cgattggagc cgtgagggca aagaccccga tcatgaggct gccaagggct
     5161 gccagcgcaa agggcgccca aatccggcgc cgcagcatca gacttccgcc ggacaaaatc
     5221 gcggtgaggc caaagatcac ggccggggca ctcatggtgc gccttgcact tcctcgggtg
     5281 ccccgctgat caagccaaga taactagcca ccagcgccaa gccgatgtga atactggcga
     5341 tcaatgccag gactgccagc gaaggttcga tgagtccata aacaacctcg aaaccggaga
     5401 gcagcgtcat gagcccaagg cacactcgga aggcggatcc cgagagtccc agttgaagca
     5461 ggcccatggt aagcagcacc gtcgagccca ggatcgcctc ttggcgcaga tcgggaactt
     5521 cgaaccagtt gcgctgaccg agcccgaacg cggcaaccag gcccaagcca gaggcgaaca
     5581 aacggaaggt tgtcccaaac tcgcggccgg caggctgagc gggcgcctgc tgctgcttag
     5641 actccgagac cgccaagatg gcgcaggcga tcatcccggc cacgagtttc accgcggcca
     5701 cttgcagcgg gagcgaaaga ccagtcagcc aagcaaccgc cgcgtactgg attgccaaga
     5761 gcccgattcg ggcaactcgc ccggtgaaca ccgtgagaag caatgcggtc cccgagacga
     5821 tcagggccgt catcccgggc tgcctccgat cgcgatgacg atggccagca cggccaggaa
     5881 catccacagg accgcactct gaccctcgag cagctcggtc cccgaacgaa tccagcgggc
     5941 gaccagctcc catgctcggc tcaagtcgtc acggatgtcg attctcaggg cgagtggcca
     6001 ccggccccca gcggaacttc ggcggggcaa gatccg
//
LOCUS       DATDPQ010000129         3548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3198124, whole genome shotgun sequence.
ACCESSION   DATDPQ010000129 DATDPQ010000000
VERSION     DATDPQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198124"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /gene="leuS"
                     /locus_tag="VI520_03070"
     CDS             complement(<1..2336)
                     /gene="leuS"
                     /locus_tag="VI520_03070"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HLF36898.1"
                     /translation="MTEAISSRQLMVDRYDPGAIEPKWRERWELDGLYAARVDPKRRK
                     FYFLTMLPYTSGNLHIGHWYAMTPSDARARYLSRKGYNVMYPIGFDAFGLPAENAAID
                     HNAHPKLWTYANIDRMRGQLRSMGAMWDWSREAVSSDPEYYRWTQWFFLQLFKHGLAY
                     KKMAPVDWCPKDQTVLAREQVIGEERVCERCGTPVVRKDLEQWFFRITKYADELLDFS
                     HLDWPEPVRLLQTNWIGRSEGARVTFRTEGEDEIPIFTTRPDTLWGATFMVLAPEHPL
                     VKLLTTAEQQHAVRKYVEKTARQTDIQREAVDKEKTGVFTGGYAVNPVNGERIPVWVA
                     DYVLMTYGTGAIMGVPAHDGRDFDFARKHGLEVRPVIRPAESGMPDGATMKEALAIYG
                     EMINSGPLTGTPQEKAITATIEYLEGHKLGERAVGYRLRDWLISRQRYWGAPIPIIHC
                     PVHGQVAVPDEQLPVQLPEDVEWLPTGQSPLKLHPSWKQAICPIGGEPAERDTDTMDT
                     FMCSSWYHLRYLSPDYSEAAFDPEQYDYWMPVDTYTGGAEHATMHLIYTRFFHKALRD
                     LGIVRGPEPMLQLRNQGHILGPDGSRMSKSRGNVIDPDEQVRDYGADSVRAYLMFGYR
                     WPEGGPWKADNIHGVVRWLNRVWTLTLESVGATAANPNPAPLRDLNRKTHQTIRAVSE
                     DLENFAFNTVVSELMELTNSIADAKQLGLAGSAEFNAAIEALLLLMAPVTPHIAEELW
                     ERLGKPYSIHTQPWPQHDPELAKDELITLVIQVNGKVR"
     gene            complement(2508..2580)
                     /locus_tag="VI520_03075"
     tRNA            complement(2508..2580)
                     /locus_tag="VI520_03075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2545..2547),aa:Ala,seq:ggc)
     gene            complement(2593..2665)
                     /locus_tag="VI520_03080"
     tRNA            complement(2593..2665)
                     /locus_tag="VI520_03080"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Ala,seq:cgc)
     gene            2691..>3548
                     /locus_tag="VI520_03085"
     CDS             2691..>3548
                     /locus_tag="VI520_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HLF36899.1"
                     /translation="MDYQERFERLSNLLRTEGLEALAVLPGPNLFYLTGLSFHLMERP
                     VVGLFSADAPPRLILPEFEQPKAEAAAVALELFSYGEQEGASLSAFQAALSRMPADGR
                     AVAVEPLRARFHELGLLQAASPHTRFVTGASLLASLRMAKDSAELAAIRRAIEIAERA
                     MDQTVPLVRLGMTEQELASELTVQLLRAGSEPEIPFSPIVATGPNSAHPHAVPGARRL
                     GAGELLVLDWGASASGYVSDLTRTYAVGEIDRELAEVHRVVEQANAAGRAAVGPGVRC
                     GEVDRAARAV"
BASE COUNT          623 a         1149 c         1065 g          711 t
ORIGIN      
        1 tcgcgtacct tcccgttcac ctggatgacc agcgtgatca gctcgtcctt ggccagctcc
       61 ggatcgtgct gcggccaagg ctgagtgtga atcgagtatg gcttgcccaa ccgctcccac
      121 agttcctcgg cgatatgcgg ggtcaccggc gccatcaaaa gcagcagggc ttcgatcgcc
      181 gcgttgaact ctgccgagcc ggccagcccc agctgcttgg cgtcggcaat cgagttggta
      241 agctccatca gctcggaaac gaccgtattg aacgcgaaat tttcgagatc ctccgatacg
      301 gctcggatgg tctggtgggt tttgcgattc aaatctcgca ggggcgccgg attcgggttg
      361 gccgctgtcg ctcccaccga ttcgagagtc aatgtccaaa ctcggttcaa ccagcggacc
      421 accccatgaa tgttgtccgc tttccagggt ccgccctccg gccaccggta gccaaacatc
      481 aaataggctc tgaccgaatc ggctccgtag tcacgcacct gctcgtccgg atcgatgaca
      541 ttgccgcggg acttgctcat ccgagaccca tcgggtccca agatatggcc ctgattgcgt
      601 aattggagca tcggttccgg accccggacg atacccaggt cgcgcagggc cttgtggaag
      661 aaccgggtgt agatcaggtg catcgtggcg tgctcggctc ccccggtata ggtgtcgacc
      721 ggcatccagt agtcgtattg ctccggatcg aacgcagcct ccgagtagtc cggactcaga
      781 tagcggaggt gataccagga ggagcacatg aaggtatcca tggtgtcggt gtcgcgctcg
      841 gccggttcgc cgccgattgg acagatcgct tgtttccagc tgggatgcag cttgagcgga
      901 ctctgtccgg tcggcagcca ctcgacatct tccggaagct gtaccggtag ctgctcatcc
      961 gggaccgcta cctgaccgtg caccgggcag tgaatgattg ggatcggggc gccccagtac
     1021 ctttgacggg agatgagcca gtcccgcagc cggtagccca ccgcacgctc gcccagcttg
     1081 tgcccttcca gatattcgat agtcgccgtg atcgccttct cctgcggcgt tccagtcagc
     1141 gggccggagt tgatcatctc accatagatg gccagggcct ccttcatagt ggccccatcg
     1201 ggcatgccgc tctccgccgg ccggatcacc ggccgaacct caagcccatg tttgcgggcg
     1261 aagtcaaaat cgcgcccgtc gtgtgccggc acccccatga tcgcaccggt gccgtaggtc
     1321 atcagcacgt agtctgccac ccagactggg atgcgctcac cgttcactgg atttactgca
     1381 tatccgccgg tgaatacgcc ggtcttttcc ttatccaccg cctcgcgctg gatatcggtt
     1441 tgccgggcgg tcttctccac atatttgcgc acggcgtgct gctgctccgc agtggtcagc
     1501 agcttcacca atggatgctc cggcgccagc accatgaaag tagctcccca caacgtatcc
     1561 ggccgagtgg tgaagatcgg aatctcgtct tcgccctccg tccggaaggt gacccgggcg
     1621 ccctcgctcc gtccgatcca gttggtctgc agcagccgca ccggttccgg ccaatccaga
     1681 tgagagaagt caagcagctc gtccgcgtac ttggtgattc ggaagaacca ctgttccaaa
     1741 tccttcctca ccaccggagt cccgcaccgt tcgcagaccc gctcctcgcc gatcacctgc
     1801 tcgcgggcca gcaccgtctg gtccttgggg caccagtcca ccggtgccat cttcttgtag
     1861 gccaagccat gcttgaagag ctgcaggaag aaccactgag tccagcggta gtactccgga
     1921 tcggaggaca ccgcttcccg agaccagtcc cacatcgctc ccatgctgcg caattgtccg
     1981 cgcatgcggt cgatattggc gtaagtccag agcttgggat gggcgttgtg gtcaatcgcg
     2041 gcgttttcgg ccggcagccc aaacgcatca aagccgatcg gatacatcac gttgtacccc
     2101 ttgcggctca ggtagcgggc ccgcgcatcg gagggggtca tggcgtacca gtgtccgatg
     2161 tgcaggttgc cggaagtgta ggggagcatg gtcaggaagt agaacttccg acgcttggga
     2221 tcgacccgag cggcatacag cccgtcgagt tcccagcgtt cccgccactt cggctcgatc
     2281 gcgcctggat catagcgatc gaccatgagt tgcctgctgc tgattgcctc ggtcacttcg
     2341 tcctccctac ccgtgtatcc cttcgcccag aatggagaac gccccttcgc cgtttcaggg
     2401 acgaaggggc gctgcgcggt cccaccctga ttgcccggac caactccggg ccactcgttc
     2461 gcctgctaga tgatggtgcg gctatgaact gtttgctgaa ttgtaggtgg acccagtggg
     2521 attcgaaccc acgaccttct caatgccatt gagacgcgct cccaactgcg ctatgggccc
     2581 ttgtgaaaca ggtggacctg gcgggattcg aacccgcgac ctcatcagtg cgattgatgc
     2641 gcgctcccaa ctgcgctaca ggcccccgct tataataccg caaatcccct atggactacc
     2701 aagaacggtt tgagcggcta tccaatctcc tgcggaccga ggggctcgaa gcgctggcgg
     2761 tgctccccgg gccaaacttg ttctatctca ccggactgtc tttccatctg atggagcgcc
     2821 cggtggtcgg cctgttctcg gctgacgccc cgccccggct cattcttcct gaattcgaac
     2881 agccaaaggc cgaggctgcg gccgtagcgc tggagctctt ttcctacggc gagcaagagg
     2941 gggccagcct ctccgctttt caagccgcat tgagcaggat gccggccgac ggccgggcag
     3001 tggcagtcga accactgcgg gcccgattcc atgagcttgg tttgctccag gctgcctcgc
     3061 cccatacccg gttcgtcacg ggagccagcc tgctggcaag ccttcgaatg gcgaaagatt
     3121 cggccgaact ggcggctatc cgccgggcga tcgagatcgc tgagcgggcc atggaccaaa
     3181 ccgtcccgct ggtccggctc ggaatgaccg aacaggaact agcctccgag ttgaccgtgc
     3241 agctgctgcg agcaggatcc gaaccggaga tcccttttag cccgatcgtc gccaccgggc
     3301 ccaattcggc ccacccacac gcggtccccg gggcccggcg gctgggcgcc ggcgaattgc
     3361 ttgtcctcga ttggggggcg agcgcttccg gctatgtctc ggaccttact cggacctacg
     3421 cggtgggaga gattgaccgg gaattggccg aggttcaccg agtcgtcgag caagccaacg
     3481 ccgcgggccg ggcggcggtt gggcccggtg ttcgctgcgg tgaggtcgac cgggcggcgc
     3541 gggcggtg
//
LOCUS       DATDPQ010000130         4463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_832079, whole genome shotgun sequence.
ACCESSION   DATDPQ010000130 DATDPQ010000000
VERSION     DATDPQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832079"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..909)
                     /locus_tag="VI520_03090"
     CDS             complement(109..909)
                     /locus_tag="VI520_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLF36900.1"
                     /translation="MASRDSGRQAAGMFAAVGPDYALVVASAGEGQRLRDLSGDRPKP
                     LLPLAGLPILHRALRSFAKQPPLRIAIVANPNWLDESQAVASGALPDVEVVGVAQSEP
                     VGSLDAFRRGLAALADPYLKQLPVALLHADNLPPEGLIEVGSKALAKSQAVLFSRLTD
                     RPGTSARLVRDERGRPVGLTERGSGRALAEIEVCGGLFLFGAWIWERFSYDQPQRRGE
                     AQIDSLDDELLRSGRAAVLPVEAAWFHINTPEDFQQAEEALRIQAVSP"
     gene            complement(893..1234)
                     /locus_tag="VI520_03095"
     CDS             complement(893..1234)
                     /locus_tag="VI520_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36901.1"
                     /translation="MITFDQTLDAVERIYEQLKSVDQVESQIDHVRANLADFASLLEY
                     AHKKEFQSTEEALQYIDKVLAPSLQGIITALVSGTEEPLKRLSTATEATKRLLGSLEL
                     VTGGSGDGLSR"
     gene            1365..1565
                     /locus_tag="VI520_03100"
     CDS             1365..1565
                     /locus_tag="VI520_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36902.1"
                     /translation="MLPIVALSLAVVFFVLLRQGILDQLPQWLRSGLQRRTPPRIAKD
                     TEEERRLEVFKDFFEGDSKPKE"
     gene            1660..2820
                     /locus_tag="VI520_03105"
     CDS             1660..2820
                     /locus_tag="VI520_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36903.1"
                     /translation="MNNKLVGFITRRLIRGLVALFLFQTLLFALVHSLPGDFASIAGA
                     FGGRAGRAAIQRRLGLDEPLGQQYLDWLWRAAHFDFGESFLYSPERVTDVLLARAPRT
                     LLVFLSAAILAYGLGLWLGKQGAWRRGGRLELATTVVSLAAHTSFAPWLGFLALNIFA
                     WNLGWFPYQHLINFNKWLGTDISIDAILLRMVLTGGAVFAAGLGSGVIVRRVRNRARR
                     IAVRTALVFATGLVCFAWWARSGHASLALDVTAHLALPLGTVVLLSFGETLLTMRATM
                     LETMGADHVTAARARGLPESILRDRHVARVAMLPVLSRLLLSLPFVLVGSLVIERVFF
                     WQAMGQVVFNAIEFQDLPLIVGVLSLVGALVLISHILLDVLYLILDPRLRYG"
     gene            2813..3757
                     /locus_tag="VI520_03110"
     CDS             2813..3757
                     /locus_tag="VI520_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36904.1"
                     /translation="MAEQQFVPPSPLVSQRRLAQRQRAEGSWQLFRGHRTGMIGLGLI
                     AIFMLLATSHPLLMSTVWDRRRYDPIVGFDMAYFPHPSRPSIDHLLGTDNFGRDVLSQ
                     LLFGARVSFGVGLTAGLVAAGLSTLQGGAAGFLGGLIDQLLMGLADVLVLMPAPVVLL
                     IFGLLVRMEWPAVGVLYGLLTGAGAQAIIVKSQTLSLRAKPYIEAAHAAGGGRLQVFR
                     RHVLPGLLPLTAVNLFLTVVGAVLTESLLSYFNRTRVDLSWGTMIWLGQETFRRHALE
                     GQWHAILPPAMAIMLFCSAFYLVGRALDEVLNPRLRAR"
     gene            complement(3766..4158)
                     /locus_tag="VI520_03115"
     CDS             complement(3766..4158)
                     /locus_tag="VI520_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HLF36905.1"
                     /translation="MVTRQSELLRVLDQMAKELPAPEWVALVDDNGLIVACIPDEPAI
                     EQERISAMTAASLIMADRVLGEIEGGQTRYTSIAGSRRQMLTVVLSRDRLLALGLGPE
                     VPAQATFSALTRWVPEILAVLKRSFVSG"
     gene            4278..>4463
                     /locus_tag="VI520_03120"
     CDS             4278..>4463
                     /locus_tag="VI520_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36906.1"
                     /translation="MRRRRLFRQRGPRLGLLLAVLYLAFLIAVAGGVPRFVQLLSAHF
                     LLSLAALFLALSMVAQFV"
BASE COUNT          705 a         1442 c         1384 g          932 t
ORIGIN      
        1 ggctgctctg acaaccattg tgactttcct gataggctat ttcgtgcccc ccagcgcgaa
       61 agacgtagtc gtaccagcct aggcctgagc ttatttgaca ggtatccctt agggcgatac
      121 cgcctgaatt cggagagctt cttcggcctg ctggaagtcc tcgggggtat tgatgtggaa
      181 ccaggccgcc tctaccggaa gcaccgccgc ccggccggac cgcagcagtt catcgtccag
      241 cgagtcgatc tgggcctcgc ctcggcgctg gggctggtcg tagctaaacc gctcccagat
      301 ccacgctcca aacagaaaca ggcctccaca gacttcgatc tcagccaacg cccgcccgga
      361 tccgcgctcg gttaatccga ccggtcgccc gcgctcgtcc cgcaccagcc gggcgctggt
      421 gccgggccga tccgtcagcc gagagaagag caccgcctgg gatttggcca gagctttact
      481 tcctacttcg atcaatcctt cgggcggaag gttgtccgca tgcagcagcg cgaccggcag
      541 ctgcttgaga tacggatcgg ccaaggcagc caatccgcgc cgaaacgcat ccagcgagcc
      601 gaccggctcg gactgggcga ctccaacgac ttccacatcg gggagcgccc cgctcgccac
      661 cgcctgcgac tcgtccagcc aattcggatt cgccacgatt gcaattcgaa gcggaggctg
      721 cttggcaaag ctgcgcaaag cccggtgcaa tatcggcagc ccggcaagcg gaaggagggg
      781 tttgggccga tcacctgaaa gatctctcag tcgctggccc tccccggcag aggctacgac
      841 cagtgcatag tctggtccaa cggccgcaaa catgcccgcg gcctggcgac cgctatcgcg
      901 agaggccatc gcccgagcct ccggtaacca actcgaggct gcccaggagg cgcttggtgg
      961 cctcggtagc tgtgctcagc cgcttgagcg gttcctcggt ccccgacacc agcgcagtga
     1021 tgattccttg caagctcggc gccaacacct tgtcgatgta ttggagggcc tcctccgtgc
     1081 tctggaactc cttcttatgg gcgtactcga gcaagctggc gaagtcggcc aggttggccc
     1141 gaacgtggtc gatctggctc tcgacctgat cgaccgactt cagctgctca tagatccgtt
     1201 cgaccgcatc cagggtctgg tcaaaggtga tcatggggtt ggttgcggtt tctgggaggc
     1261 tccggtttga tcatacctga gaacccgtcg cctctcaatc ggacctccgg cccgccgggg
     1321 tcaccggccg gctgggatat aatgggccgc gagggctgac cgagatgctg ccaattgttg
     1381 ccctgagcct ggcagtcgtt ttcttcgtcc tgctcaggca ggggatcctg gatcaactgc
     1441 cgcagtggct tcgcagcggt ctgcagcgcc gcactccacc gcgcatcgct aaagacaccg
     1501 aggaagaacg gcggctggag gtcttcaagg actttttcga aggcgattcc aagcctaaag
     1561 aatgatcacg gggagctgag cagcaaggcg accggaccgg ctgggtccgg ttttttcgtg
     1621 gtggtgaacc ccgccctagc ccgaggtggt aatcgggtag tgaacaacaa gctggtcggt
     1681 ttcattaccc ggcgcctgat ccgtggactg gtcgcccttt tcctctttca gacccttctc
     1741 tttgcgctgg ttcactccct ccccggggac ttcgcctcga tcgccggggc cttcggaggc
     1801 cgcgccggcc gggccgcgat ccagcgtcgg ctcggattgg acgaaccgct cgggcagcag
     1861 tatctggact ggctgtggag agctgcccac ttcgactttg gcgagtcatt cctctactct
     1921 ccggagcgag tcaccgatgt gctgctggcc cgggctccgc gcacgctgct ggttttcctc
     1981 agcgcagcca tcctcgccta cggtctcggg ctgtggctcg gcaagcaggg ggcctggcgc
     2041 agaggcggca ggctggagct ggcgaccacc gttgtcagcc tggccgccca cacctccttt
     2101 gcaccgtggc tcggatttct ggccctaaat atcttcgcct ggaacctggg ctggttcccg
     2161 taccagcatt tgatcaactt caacaagtgg ctgggcaccg atatctcgat cgacgcgatt
     2221 cttctccgca tggtgctgac gggcggggca gtcttcgcgg ccgggctggg ctccggcgtg
     2281 atcgtccggc gagtgcggaa ccgagcccgc cgcatcgccg tgcggaccgc gctcgttttc
     2341 gccaccgggc tggtctgctt cgcctggtgg gcgcgctccg ggcacgccag cctggccctg
     2401 gatgtcacgg cccacctggc actaccgttg ggcacggtgg tgctgctgtc ctttggagag
     2461 acgctgctga cgatgcgggc gacgatgctc gaaaccatgg gcgccgatca cgtgactgcc
     2521 gcccgagcca gaggtctgcc ggagtccatt ctgcgggatc gacacgtggc ccgggtggcc
     2581 atgctgccgg tgctgagcag gctgctactt agcctaccgt ttgtgctggt gggcagcctg
     2641 gttatcgagc gagtcttctt ctggcaggcc atggggcagg tggtgttcaa tgcgatcgag
     2701 ttccaagatc tgcccctgat cgtcggagtg ctcagcctgg tcggggcgct ggtgctgatc
     2761 tcgcacatcc tgctcgatgt gctctacctg atccttgatc cccggctgcg ctatggctga
     2821 gcagcaattc gtgcccccct ctccgctcgt ctcgcagaga aggctggcgc agcggcagcg
     2881 ggccgagggc tcctggcagc tcttccgggg gcatcggacg ggaatgatcg gactgggtct
     2941 gatcgcgatc tttatgttgc tggcgaccag ccatccactg ctcatgtcga cagtttggga
     3001 ccgacgccga tacgatccga tcgtcggctt tgacatggct tacttccccc atcccagccg
     3061 accttcgatc gatcacctgc tgggaaccga caatttcggc cgggatgtgc tgagccagct
     3121 gctgttcggg gctcgggtgt ccttcggggt tgggctcacg gccgggctgg tggcagccgg
     3181 gctctcgaca cttcagggtg gggctgcggg ctttctgggc ggtctgatcg accagctctt
     3241 gatgggcctg gccgacgtgc tggtgctgat gccggctccg gtagtgctgc tgatcttcgg
     3301 tttgttggtt cgaatggagt ggccggcagt gggtgtgctc tatggcctgc tgaccggtgc
     3361 cggcgcccag gcgatcatcg tcaagtcaca gaccctctcg ctgcgtgcca agccctatat
     3421 cgaagccgca cacgcggccg gcggaggccg gctccaagtc ttccggcggc atgtgctgcc
     3481 tggcctgctg ccgctcaccg cggtcaatct ttttctcacc gtggttggcg cggtgcttac
     3541 cgaatcgctg ctctcctatt tcaaccgcac tcgggtagac ctcagttggg gcacgatgat
     3601 ctggctcggc caggagactt ttcgccggca cgccctggag ggacaatggc acgccatcct
     3661 tcccccggcg atggcgatca tgctcttttg cagtgccttc tatcttgtgg ggcgagcgct
     3721 ggacgaagtg ttgaatccgc ggctgcgggc gcgctgaagc ctagatcacc cggagacaaa
     3781 actacgcttc agcaccgcca ggatttcagg gacccagcgg gtcagcgccg aaaaggtcgc
     3841 ttgggccgga acttccgggc cgagtcccag tgctagcagc cggtctcggc tgaggaccac
     3901 cgtcagcatc tgccggcgcg aacctgcgat gctcgtgtag cgggtctggc ccccttcgat
     3961 ttcccccagg acccgatcgg ccatgatcag tgatgcggcg gtcatggcgg agatccgctc
     4021 ctgctcgatc gccggttcgt cgggaatgca cgccacaatc agcccgttgt catccaccaa
     4081 cgcgacccat tccggcgcag gcagttcctt ggccatctgg tccaatacgc gcaacaattc
     4141 agactgacgg gtaaccatcg tctctctcag caaagagtac acctaaccag cgacttggcc
     4201 cttagagtat cctctcaaag tcggccgggc aagccttagt tttctcgctg gttcctttga
     4261 tatacaatcc ggcccccatg cgccgacgaa gactcttccg ccaaaggggt ccacggctcg
     4321 gcctgctgct tgcggtcctg taccttgcat tcctgatcgc ggtcgccggc ggcgttccac
     4381 gcttcgtcca gctgctgagt gcccacttcc tgctctcgct cgccgcgctg tttcttgccc
     4441 tttctatggt ggctcagttc gtg
//
LOCUS       DATDPQ010000131         3814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2664556, whole genome shotgun sequence.
ACCESSION   DATDPQ010000131 DATDPQ010000000
VERSION     DATDPQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2664556"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VI520_03125"
     CDS             complement(<1..192)
                     /locus_tag="VI520_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36907.1"
                     /translation="MTNRFLIYSALILLLVAGLAACGGPSTAAIAMSMSEFSFSPASA
                     SVPAGAEVTLTLTNSGTIEH"
     gene            321..1019
                     /gene="coxB"
                     /locus_tag="VI520_03130"
     CDS             321..1019
                     /gene="coxB"
                     /locus_tag="VI520_03130"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLF36908.1"
                     /translation="MRERNILLVTILVLSLIGVGIAVASLVRPLPQVASDRAVLVDQL
                     LRVFFGLATAVFLGVEGLLVFAAVRGRLFGSSAAIGNPNPALELLWLAVPAAIVLVLS
                     VYSIRVLLLMEAPRSDPLLVEIAASQYRWEFHYPESGVTTNELHLPAGRPAVLTFRSS
                     DVIHSFWVPVFGGKVDALPDRVTSLALTPLRTGVYQAVCAEYCGADHAGMIAPVWVED
                     PAGFEQWLQSEGGD"
     gene            1022..2485
                     /locus_tag="VI520_03135"
     CDS             1022..2485
                     /locus_tag="VI520_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLF36909.1"
                     /translation="MSRGMRSYLRPTTDPAVLAVQFLVTSFAFFLLGGALALGMAAEA
                     SVPGVELMGAEAYSRAFTNHGSIMIFLWLMPAVAGLTHALLLRVLGATRSALPRLSGL
                     GFWLLLPGGLLMLSSFFMGGDSPGWTAYLPLSAQTTGVGQTLWVASLIVLLLSLGCNA
                     TGFLATILAARSEKSRSRSPFVVASLISSTLALISAPVLLAALTALLVDRLSAGPSFL
                     AGSLGVTLWQNAFWFFGHPATTMLLLPAIGAVSEVIQANTGNPLVAKRSVNLAMVAVG
                     GLGLVSWGQHMFASGLIPAVRVPFMLTSMAVAIPLAFIVFAWLTTLWVSKVRFSTPML
                     FSLGFISMFGLAGLSGLVLASIPANLQLHGSTFVSGHSHLALFGGVMSGLFAGIYYVF
                     PTLTGKNYDENLGKLHFLAHAGGVHLAFLPMLLIGLQGVPRRVFEIRPGLELFELASS
                     GGALILAGASLLFLYNIFFAWRAGRPAAQEVLPGSTL"
     gene            2482..3384
                     /locus_tag="VI520_03140"
     CDS             2482..3384
                     /locus_tag="VI520_03140"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HLF36910.1"
                     /translation="MTTGLTEPQTSTWSTRLVDYLSLTKPAIVALLLLTTLCGMVIAI
                     GGLPPLATVGLTLLGGGLTAGGAGALNQYIDRGIDRRMVRTMRRALPDQRIRPVEGLL
                     FGLAMCVAGLAVLAIGVNLLSAILSLAGIIYYAVLYTVLLKPSTPQNIVVGGGAGALP
                     PLVGWAAATGSLSLPAFFLFAVIFFWTPPHFWALALLKRSDYARAGIPMLPVVSGEVE
                     THRQILLYSLQLVALTALLPLVGLGGGLYLTAALLLGIGLVRHAWILQRRGGNRFAWR
                     MYRYSSLYLAGIFLALAADAVLIG"
     gene            3425..3757
                     /locus_tag="VI520_03145"
     CDS             3425..3757
                     /locus_tag="VI520_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLF36911.1"
                     /translation="MVETIRMEKVDVVKAAGRIDSHSSPELKDAFDLLLNDGRNMLVF
                     DMSEVEFLSSSGVWVLLETQKRARRRSKGDLVLANPSENIKKTLDLAGLMHFMKIFDG
                     VDTAVAGF"
BASE COUNT          605 a         1127 c         1208 g          874 t
ORIGIN      
        1 gtgctcgatc gtcccactgt tggtcaaggt gagcgtgact tcggctccgg ccggcactga
       61 ggcgcttgcc ggtgagaagg agaattccga catggacatg gcaatcgctg cggtgctcgg
      121 acctccgcag gcagccagtc ccgccacgag caagagaatc aaggcagaat agatcagaaa
      181 ccggttggtc atagggatct tcctcctgtt gatgcgagag tctaagccgg tccgtccgag
      241 agcgcaagca acatgcccgc tcgggtcccg attcgacgga gccagccgat gataaactac
      301 cagccgccag cacgggagcc atgcgcgaac gcaatattct gctcgtaacg atcctagtcc
      361 tgagcctgat tggcgtgggg attgccgtgg cgagcctggt tcgacccttg ccgcaggtcg
      421 ccagcgaccg tgccgttctg gttgaccagc tgctccgagt cttcttcggc ctggcaaccg
      481 cggtgttcct cggggtggag gggctgctcg ttttcgcggc cgttcgcggg cgactcttcg
      541 gtagctcagc tgcgatcggc aacccgaacc cagcattgga gctgctgtgg ctcgcggtcc
      601 cggccgcgat cgtgctggtg ctcagcgtat actcgatccg agtgctgctg ctgatggaag
      661 cgcctcgatc cgatccgctg ctagttgaaa tcgcagcgag ccaataccgg tgggagttcc
      721 attacccgga aagcggcgtt actacaaacg aacttcacct gcccgcgggg cgcccggccg
      781 tgctgacatt cagatcgagc gatgtgatcc actctttctg ggttccggtc tttgggggga
      841 aggttgacgc tctgccggac cgggttactt cgctggcctt gaccccactt cgcactggag
      901 tgtaccaagc tgtttgcgcc gagtactgcg gcgcggatca cgccggaatg attgctccgg
      961 tgtgggttga ggatccagcc ggtttcgaac agtggctgca atctgagggc ggagattgat
     1021 catgagcaga ggcatgcgca gttatcttcg tccgacaacc gatccggcag tgctggccgt
     1081 ccagttcctg gtgaccagtt tcgccttttt cctgctgggg ggtgcgctgg cgctcggaat
     1141 ggctgccgag gccagtgtgc ccggggtcga gctgatgggt gccgaggctt actctcgagc
     1201 cttcaccaat catggctcga tcatgatctt cctttggctg atgccggcgg ttgccgggct
     1261 cacccacgcg ctgctgctgc gagtgcttgg tgcgacccga agcgccctcc caagattgag
     1321 cgggctggga ttctggctgc tcctgcccgg cggactgctg atgctgtcca gcttctttat
     1381 gggcggggac tcccccggct ggacggccta tctgccgctg tcggcccaga cgactggggt
     1441 gggccagacc ctgtgggtcg ccagcctgat cgtgctcctt ctttcgctgg gctgcaacgc
     1501 gaccggattc ttggccacaa ttcttgccgc tcggagtgaa aaatcccgca gtcgctctcc
     1561 atttgttgtc gccagcctca tcagctcgac cctggcgttg atttctgccc ccgtcctact
     1621 ggcggcactg accgcgctgc tggtggaccg cctgagcgcg ggccctagct tcctcgccgg
     1681 ctcgcttggg gtgaccctgt ggcagaacgc attttggttc ttcggccacc cagcgacgac
     1741 aatgctgctc ctgccagcga ttggggcagt ctcggaggtc attcaggcca acaccggcaa
     1801 cccgttggtc gcaaagcgat cggtcaacct tgccatggtc gcggtcggcg gcctgggcct
     1861 cgtctcctgg ggtcaacaca tgtttgccag cggcctgatc ccggccgtcc gggtcccgtt
     1921 catgttgact tcgatggcag tcgccattcc tctggctttc atcgttttcg cctggttgac
     1981 taccctgtgg gtcagcaagg tgcggttcag cactccgatg ctgttttcgc tcggtttcat
     2041 ctcgatgttc gggctggcgg ggctcagcgg tttggtcctg gcttcgatcc cggccaacct
     2101 tcaactgcac ggcagcacct tcgtctccgg ccactcgcat ctcgcacttt tcggcggagt
     2161 gatgtcgggg ttgttcgccg ggatctacta tgtatttccg actctcaccg gaaaaaacta
     2221 tgatgagaat cttggaaagc tgcacttcct cgcccacgcc ggtggtgtgc atctggcttt
     2281 cttgccgatg ctgctgatcg ggttgcaagg tgtcccgcgc cgggtctttg agatccggcc
     2341 tgggctcgaa ctctttgagc tggcttcgag cgggggcgcg ctgatcctgg ccggcgcctc
     2401 gctgctgttt ctctacaaca tattctttgc ctggcgggcc ggccgacccg cagcccaaga
     2461 agtcctgccg gggtcgaccc tgtgaccacc ggcctgaccg agccccaaac ttcgacctgg
     2521 tccactcgac tggtggatta tctatcgctc accaagccgg caatcgtcgc tttactctta
     2581 ttgacgacct tgtgtgggat ggtgattgca attgggggac ttccgccgct ggcgacggtt
     2641 gggttgaccc tgctcggggg tggactcacc gcaggagggg cgggggcgct caatcagtac
     2701 atcgaccggg gtattgaccg gcggatggtt cgaacgatgc ggcgggccct cccggaccag
     2761 cgtatccggc cggtggaagg tctgttgttc ggtctggcga tgtgtgtggc tggcctggcc
     2821 gtcctggcga ttggcgtgaa tctgctcagt gcaatcctct ctttggcagg catcatctac
     2881 tacgcggtgc tctacactgt cctgctgaaa ccgagtaccc cgcagaatat cgttgtcggt
     2941 gggggagcgg gtgcattacc gccactggtg ggatgggctg ccgccaccgg atcgctcagc
     3001 ctgccggcat ttttcctatt cgcggtgatc ttcttttgga ccccgcccca cttttgggcc
     3061 ctggccctgc tcaagcggtc tgattacgcc cgggccggaa taccgatgct gcccgtggtc
     3121 tccggcgagg tcgagaccca tcgtcagatt ctgctgtaca gtttgcagct ggtcgcgcta
     3181 acggccctgc tcccgctggt ggggttgggc ggggggctgt atctgaccgc tgcgctgctg
     3241 ctcgggatcg ggttggtccg ccacgcctgg atcttgcaaa ggcgcggtgg gaatcgattt
     3301 gcctggagaa tgtatcgtta ctcgagcctg tatttggcag ggatcttttt ggcgctggcg
     3361 gccgacgcgg ttctcatcgg gtgacatgat aaactttggc cgatcgagcg actcaggagg
     3421 agcaatggtt gagacaatcc gaatggaaaa agtcgacgtg gtgaaggctg ctgggaggat
     3481 cgacagccac tcttcgcctg agctgaagga cgcatttgac ctcttgctca atgacggacg
     3541 caacatgcta gtcttcgaca tgagcgaagt ggagttcctc tctagtagcg gggtgtgggt
     3601 cctgttggag acccagaagc gagcccgccg ccgcagtaag ggagatctgg tgctggccaa
     3661 cccgagtgag aacattaaga agaccctcga tttggccggg ctgatgcact tcatgaagat
     3721 cttcgacggt gtcgacactg ccgtggctgg tttctgaagt tgagcattga tgcgtaatgc
     3781 ccaaggcgac gttcaaggct cgatacgaga acct
//
LOCUS       DATDPQ010000132         8166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_313108, whole genome shotgun sequence.
ACCESSION   DATDPQ010000132 DATDPQ010000000
VERSION     DATDPQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313108"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VI520_03150"
     CDS             complement(<1..1133)
                     /locus_tag="VI520_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HLF36912.1"
                     /translation="MAESLATYGLEHHGLNHEHEVYWGLPAPALVEHIARRAEGQISD
                     SGAIVVRTGAHTGRAPKDKFVVREGSSTEQVWWGNVNQPLEPDRFEALHARVARYLDS
                     KPLYIQDLAVGAMPEFRIPIRVVSESAWHSLFARNLFLRPPADGLADHIPEFTVLHAP
                     GFKADPARDGTHSEVFIVLNFEQGLALIGGTSYAGEIKKSLFTVMNYRLPLQGVLSMH
                     CSANQGHAGDVALFFGLSGTGKTTLASDPTRRLIGDDEHGWSDKGVFNFEGGCYAKTI
                     RLSAKYEPVIWEAVHDFGTVLENVVMDETSRRVDLDDDSLTENTRAGYSLEAVENYVP
                     EGSGGHPATVFFLTADASGVLPPISRLTPEQAQYYFLAGYTSKL"
     gene            complement(1185..2618)
                     /locus_tag="VI520_03155"
     CDS             complement(1185..2618)
                     /locus_tag="VI520_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLF36913.1"
                     /translation="MGGGQEGIGLIGFGLKGLSRLALVGGLTVLACSRADLPFNPSQT
                     LAPPPATIAPSQIPSLLPSSVPGSVTEQPPEPTPSPSPSATATAIPTATPATEIGYTT
                     AAAQVGYRIPLTLRQVTPDSAVLFLELEIPAAGFVLYRPIATAAAGWFLQALDLESNR
                     HQIELVGLTPGAEYQVRVGLGESLADLRAPWLYGSRWGDLVFETPALDPPELRIGVIG
                     DSGYGQSATQDLAELMASYRLDFVLHTGDLAYRVNEEEDVFEGYAKKYFLSLAPLLTT
                     LPVYPVPGNHEYDRETYWLDRPFYFYAFAGVGIQGVANESWYSFSAAGIQFLMLDSQV
                     FHGAAGRASQTAWLAERLADPNFLFSIPVFHTPPFTSGLHRNDGKALAAEWLPQFEAA
                     GVPLVFSGHDHNFERILFNGVTYVVSGGGSRTLYPESGPVPGSQTFAMQTHFVLLEIT
                     ADRISVEAINLDNEVLDRATIELEPAD"
     gene            complement(2588..4009)
                     /gene="mazG"
                     /locus_tag="VI520_03160"
     CDS             complement(2588..4009)
                     /gene="mazG"
                     /locus_tag="VI520_03160"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLF36914.1"
                     /translation="MTEQSLTVVGLGPGNPRMITREAWNALETAGTVYLRTATHPAVA
                     GLPPDVELVSFDDTYERYSDFGDVYRAIVERLSRLSPGAVYAVPGDPSVGEATVQGLR
                     QAGFKLRVIPGISFMEPSLAALGLDALDGLVIVDAIGLAAGHHPPFPPDLGALLCQLH
                     SPLLAGELKLSLMNQYPEEHPVALIHRASLADERVEWVKLYEIDRSDSIGDLTSLYVP
                     ALPLDSSFETLQNTVARLRAPDGCPWDREQTHQSLRKHLLEEAYEALAALDEDDLGDL
                     KEELGDLLLQIVLQVQIATEQSNFRMADVVAGIQVKLIRRHPHVFGDLQVSGIDQVLR
                     NWEHFKEQETGTGPLAGVPRSFPALAQAAELQGRASRLGFDWPSAAGVRVKVQEELDE
                     IDAAADPQAQAREIGDLLFTVVNYARWLDADPESELRQANQRFRSRFDRMTALSREQG
                     SRLEDLPLEEMNRLWEAVKRELG"
     gene            complement(4102..4494)
                     /gene="rpsI"
                     /locus_tag="VI520_03165"
     CDS             complement(4102..4494)
                     /gene="rpsI"
                     /locus_tag="VI520_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLF36915.1"
                     /translation="MTDRYYEGIGRRKNSTARVRIVSGSGAIVINEKPGAEYFPRLGD
                     LDRVLAPLRAVQYEGKLDISAAVSGGGVSGQTTAVQHGVARALVKMDATLRPILRQAG
                     MLTRDARVKERKKPGLKRARKAPTYTKR"
     gene            complement(4494..4946)
                     /gene="rplM"
                     /locus_tag="VI520_03170"
     CDS             complement(4494..4946)
                     /gene="rplM"
                     /locus_tag="VI520_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLF36916.1"
                     /translation="MQKTFHPKRSELQQDWLLVDAAGENLGRLATQVARRLLGKDKPT
                     FTPGAEVGDHVVVINASKIQVSGKRLDQKFYYRVSGYPSGIKKVSLRDQLAHTPERAI
                     RAAVWGMLPHNRYGRKLIGKLKIYRGADHPHAAQQPVEVVLTNKKAAQ"
     gene            complement(4957..5718)
                     /gene="truA"
                     /locus_tag="VI520_03175"
     CDS             complement(4957..5718)
                     /gene="truA"
                     /locus_tag="VI520_03175"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLF36917.1"
                     /translation="MGTRADTALYKSIVAYDGTAFLGFQRQREGSRTVQIVLEQALRK
                     LGWQAGSLRAAGRTDAGVHALGQVVDYELSWGRGPDRLTDAINSQLPPDLAVRSTELA
                     PSGFHPRFSALRRRYSYRLLLSGRADPLVERFAWRVWPQPDLERLAAASRLFVGEHDF
                     GSFGRPTRPGGNTVRRLWTAEWSREGDQLCLRVEADSFLNRMVRRMVGAIVGVGRGLA
                     EPEEIEGRIRNPGQVWPGRPAPARGLCLEEVTYPV"
     gene            complement(5722..6090)
                     /gene="rplQ"
                     /locus_tag="VI520_03180"
     CDS             complement(5722..6090)
                     /gene="rplQ"
                     /locus_tag="VI520_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HLF36918.1"
                     /translation="MRHRVGGRRLNRSIGNRTALRRNLITELFRRERIRTTKAKAAAI
                     RGDSERLITLAKRAGQEKQVHARRLAAARLNDPQVVKKLFDDLAPRFQDRPGGYTRML
                     KLGPRTSDGAEMVLLELVEE"
     gene            complement(6087..7052)
                     /locus_tag="VI520_03185"
     CDS             complement(6087..7052)
                     /locus_tag="VI520_03185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLF36919.1"
                     /translation="MPKIERDAIARNYGRFIISPLESGYGITLGNALRRVLLSSLEGA
                     AVTSVRFSDVQHEFSEVPGVREDVVQAMLNFKQLRLVLHEGETARLRLEVRGEGVVTA
                     ADVIAPAEVEIVNPELYLFTVDDAKANLEIELNVERGRGYSPSDERARLPIGELPTDA
                     IFGPVRKVNFEVGQARVAQDTSFDRLTMEIWTDGTLRPEQALSRSATILIAHLRDLAG
                     VTEESLIAAATREEEPKMASEVYEIPIENLDLSVRVFNSLKRTGITSVGEVLDMLEKG
                     QDAMLSIRNFGEKSLDELKERLREKGYLSDETSAELEPETAVAEG"
     gene            complement(7166..7804)
                     /gene="rpsD"
                     /locus_tag="VI520_03190"
     CDS             complement(7166..7804)
                     /gene="rpsD"
                     /locus_tag="VI520_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLF36920.1"
                     /translation="MAKYTGSVCKLCRREGEKLFLKGQRCYTPKCAFERREYPPGDHG
                     RQAQWRRRRVSEYWRQLREKQKTRRVYNVTERQFRRYYAEALQTRGMTGVNLLGMLER
                     RLDNVVYRLNFAESRAQGRLLVTHGHFSVNGRRTDIPSMLIKPGDVIEVREGSRKRTY
                     FQNLLQSAESRAAPKWLQRDSTSLSARVIQNPERGDIDANLNEQLIVEFYSR"
     gene            complement(7858..>8166)
                     /gene="rpsK"
                     /locus_tag="VI520_03195"
     CDS             complement(7858..>8166)
                     /gene="rpsK"
                     /locus_tag="VI520_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLF36921.1"
                     /translation="NNTIITITDPQGNAVVWGSAGSRGFKGSRKSTPFAARTAAEVTI
                     RSARDLGLQEVDIFVNGPGPGRESAIRAIQSSGVRVRSISDVTPIPHNGCRPPKKRRV
                     "
BASE COUNT         1462 a         2634 c         2472 g         1598 t
ORIGIN      
        1 agcttggagg tgtagccggc cagaaaatag tactgcgcct gttccggagt cagccgtgag
       61 atcggcggca gcactcccga ggcgtctgcg gtcagaaaga acaccgtggc ggggtgaccc
      121 ccgctcccct ccgggacata attctccact gcctccaagg agtaaccggc ccgagtgttc
      181 tcggtcaggc tgtcgtcatc caggtcgacc cggcggctgg tttcgtccat gacaacgttt
      241 tccagtacgg tgccaaagtc atggactgcc tcccatatca ccggctcgta tttcgcgctc
      301 aggcggatgg tctttgcgta gcatccgcct tcgaaattga atacgccctt gtcactccag
      361 ccgtgttcgt cgtcgccgat caagcgtcgg gtcggatccg aagctagggt ggttttcccc
      421 gttccagaca agccgaagaa cagcgccaca tcgccggcgt ggccttggtt agcggagcag
      481 tgcatggaca gtacgccttg cagcggcaga cggtagttca tcactgtgaa aagggatttc
      541 ttgatttccc ccgcgtaact cgttcccccg atcagggcga gcccctgttc aaagttcagg
      601 acgatgaaga cttccgaatg ggtcccgtcg cgggccggat ccgccttgaa tcctggggcg
      661 tgcagcaccg tgaactcggg aatgtgatcc gccaaaccat ctgccggagg tcgcagaaac
      721 aggttgcgag cgaacaagct gtgccatgca ctctcgctca ccacccggat cgggattcga
      781 aattcgggca tggccccgac cgccaggtct tgaatgtaga gcggcttcga gtcgagatag
      841 cgggctaccc gcgcatgcag cgcttcgaag cggtccggct cgaggggctg gttgacattt
      901 ccccaccaga cctgctcggt gctcgagccc tcgcggacca cgaacttatc cttcggtgcc
      961 cggccggtat gggcgccggt tcggacgacg atcgccccgg aatctgagat ctggccttct
     1021 gcccggcggg cgatgtgctc gaccagcgcc ggggccggca ggccccaata gacttcatgc
     1081 tcatggttca acccgtggtg ttcgagtccg taggtggcaa gcgactcggc cattggcgac
     1141 cgatcttatc atggacggct cgcgctcgat gagcgcctgc ctgcctagtc cgccggctcg
     1201 agctcgatcg tggcgcggtc gagcacttcg ttgtccaggt tgatcgcctc gaccgagatg
     1261 cggtcagcgg tgatttccag cagtacgaag tgggtctgca tcgcgaaagt ttgactgccg
     1321 ggaacaggcc cggattccgg gtagagggtc cgcgagccgc cgccgctgac aacataggtc
     1381 acgccattga agagaatacg ctcgaagttg tggtcgtgac cggagaagac gagcggaacc
     1441 ccggccgctt cgaactgggg cagccactct gcagcgagcg ctttgccatc gttccgatgg
     1501 agcccgctgg tgaacggcgg agtgtggaag accggaatcg aaaagaggaa gttcgggtcg
     1561 gccaaccgtt ccgccagcca ggcggtctgc gacgcccggc ctgccgcacc gtggaaaacc
     1621 tgcgaatcca gcattaaaaa ctggatcccg gccgccgaga atgaatacca cgactcgttg
     1681 gcgacaccct ggattccgac gccggcaaag gcgtagaagt agaagggccg atccagccaa
     1741 taggtttctc ggtcgtactc atgattgccc gggaccgggt agaccggcag ggtggtcagc
     1801 agcggcgcca ggctcaggaa gtatttcttg gcgtacccct cgaacacgtc ctcttcttca
     1861 ttgactcgat aggccaggtc tccggtgtgg agcacgaaat ccagccggta gctggccatg
     1921 agttcggcga gatcctgagt ggcagactgg ccatatccgc tgtccccgat gaccccaatc
     1981 cgcagctctg gcgggtccaa cgcgggggtt tcaaagacca gatctcccca gcggcttcca
     2041 tacagccagg gggcacgcag atcggcgagg ctctccccca ggccgacccg cacttgatac
     2101 tcagctccag gggtaagtcc aacgagttcg atctgatgtc ggttactttc taggtcgagc
     2161 gcttggagga accagccggc agccgcggtc gcgatcggcc gatacagcac gaacccggcg
     2221 gcaggaatct ccaactcaag gaacaagact gccgaatcgg gagtgacctg gcgcagggtc
     2281 agcgggatcc ggtagccgac ctgggcggcc gcagtggtgt agccaatctc ggttgccgga
     2341 gtggccgtcg ggatcgcggt ggccgtagct gaggggcttg gagacggggt cggttccggc
     2401 ggctgctcgg tgactgaccc tgggacagac gaagggagca ggctgggaat ctgagatggt
     2461 gcaatggtgg caggcggagg ggctagggtt tgggaagggt taaacggcag gtctgctctg
     2521 gagcaagcca gaacagtcag gcccccgacc agcgccagcc gggagagtcc tttgagccca
     2581 aatccgatca acccaattcc ctcttgaccg cctcccacag ccggttcatc tcctccagcg
     2641 gcaagtcttc gagccgggaa ccctgttcgc gggagagcgc ggtcatccgg tcaaatcgag
     2701 accggaatcg ctgattggct tggcgcagct cggactccgg atccgcgtct aaccagcgag
     2761 cgtagttgac aaccgtaaag agcaggtctc cgatttcccg ggcctgggcc tgcgggtcgg
     2821 cggctgcgtc gatctcatcc agctcttcct ggactttcac ccggaccccg gctgcgctgg
     2881 gccagtcgaa gccgagccgg ctagccctgc cctgcagttc cgcagcctgg gccagagcag
     2941 ggaaggaacg tgggacgccc gccagcggac cggtcccggt ctcttgctcc ttgaagtgct
     3001 cccaatttct taagacttgg tcgatgccgc tgacctgcag gtcgccgaag acatgcgggt
     3061 gacgccggat tagcttgact tgaatcccag ccaccacgtc cgccatcctg aagttggatt
     3121 gctccgtggc gatttgaacc tggagtacga tttggagcag caaatctccg agctcttcct
     3181 tcaggtcgcc taggtcatct tcgtccaggg cggccagcgc ctcgtaggct tcttccagca
     3241 ggtgcttgcg gagcgactga tgggtttgct cccgatccca ggggcaaccg tcgggtgccc
     3301 gcagccgggc cacggtattc tgcagcgtct cgaaggagga gtcgagcggc agggccggaa
     3361 cgtacaagct ggtgagatcc ccaatcgagt cgcttcgatc aatttcgtag agcttgaccc
     3421 actcgacccg ttcatcggcc aggcttgccc ggtggatcag ggcgaccgga tgctcctccg
     3481 gatactgatt catcagcgac agcttgagtt cgccggctag gagcggggag tggagttggc
     3541 acagcagtgc ccccaggtcc ggggggaacg gtggatgatg cccggctgcc aagccgatcg
     3601 cgtcgacgat caccagcccg tcgagcgcat ccaggccgag ggcggccagg ctcggttcca
     3661 tgaaactgat cccagggatc acccgcagtt tgaatccggc ctggcgcagc ccttggaccg
     3721 tcgcttcccc gacgctgggg tcgccgggca ccgcgtacac tgcaccgggc gacagcctgc
     3781 tcagccgttc gacgatggcc cggtatacgt cgccgaagtc cgagtagcgc tcgtaggtat
     3841 cgtcgaagga aaccagctca acgtctggag gcaggccggc gaccgcgggg tgggttgcgg
     3901 tccgcaggta gaccgtgccg gcagtttcca gggcgttcca ggcctctcgg gtgatcatcc
     3961 gtgggttgcc cggtccgaga ccgactaccg ttaaactctg ctcagtcaag ccaacctcct
     4021 caaaatgatc aaggccgagg cgatcgttcc gcctcggcct tggttggggc catctttccg
     4081 gagccgaccg atgcccgaag ctcagcgctt ggtataggtc ggagccttgc gtgcccgctt
     4141 gagaccgggc ttcttgcgtt ccttaacccg cgcatctcga gtcagcatgc cggcctggcg
     4201 caagatcgga cgcagggtag catccatctt gaccaatgct cgcgccactc catgctggac
     4261 ggcggtcgtc tgaccggata ctccgccgcc cgatactgcg gccgagatgt ccagcttccc
     4321 ttcatactgc accgcccgca gcggagccag cacccgatcc aggtctccaa gccgcggaaa
     4381 gtactccgct ccaggttttt cgttgatcac gatcgcccca ctcccggaaa cgatccggac
     4441 ccgagctgtg ctgttctttc tccgtccaat cccttcgtag tatcgatctg tcatcattgg
     4501 gcagccttct tattcgtaag tacgacctca accggctgtt gtgccgcatg cgggtggtcg
     4561 gcgccgcgat agatcttgag cttcccgatc agcttgcggc cgtatcgatt gtgcggaagc
     4621 attccccaaa cggccgcccg gatggcccgc tccggagtgt gcgccagctg atcacgcagc
     4681 gagaccttct tgatcccgct agggtaaccg ctgacccgat agtaaaactt ctggtcgagc
     4741 cgcttccccg acacttgaat cttggaggcg ttgatcacca ccacatgatc cccgacctcg
     4801 gccccaggcg tgaaggtcgg cttgtctttt ccgagcaacc gccgagccac ctgggtagcg
     4861 agccggccca aattctctcc ggccgcgtcc accagcagcc aatcctgctg aagctcactt
     4921 cgtttcggat gaaatgtttt ctgcaaagct gcctcgctac accgggtacg tcacttcttc
     4981 aaggcaaagt ccgcgggccg gggccggccg gcctggccag acctggcctg ggttccggat
     5041 ccggccctcg atctcttccg gctcggcaag ccctcgaccg acccccacga tcgcgccgac
     5101 catccgccgg accattcgat tgagaaatga gtccgcctca acccgcaggc agagctggtc
     5161 gccctcccgg ctccactccg cggtccaaag cctgcggact gtgttcccgc caggccgggt
     5221 cggacggccg aaacttccaa aatcatgctc cccgacaaac aatcgactgg ctgccgcaag
     5281 ccgttcgaga tccggttggg gccagacccg ccacgcgaac cgttctacca gcgggtctgc
     5341 ccgcccgctc aagagcaatc ggtaggagta ccggcgccgc agtgccgaga accgcggatg
     5401 aaaaccgctc ggcgccagct cggtcgagcg cacggccaga tcggggggca gctgcgaatt
     5461 gatggcatcc gtcagtcgat ccggcccgcg accccaggac aattcatagt cgaccacctg
     5521 gccgagcgca tgcacgccag cgtcggtgcg ccccgccgca cgaagcgatc cggcttgcca
     5581 tcctagtttg cggagcgcct gctccagcac aatctgaacg gtgcgggagc cttcccgctg
     5641 acgctggaaa cccaggaagg cggtgccatc gtaggcgaca attgatttgt aaagagcggt
     5701 atcggctcga gtccccagcc gctattcttc caccagctct aaaagcacca tctcggcccc
     5761 gtcactggtg cgggggccca gtttcaacat ccgggtgtat ccgcccggtc gatcctgaaa
     5821 cctgggagcc aggtcgtcga atagcttttt gaccacctgc gggtcgttga ggcgagcagc
     5881 cgccagtcgg cgggcgtgga cctgcttctc ctgcccggcc cgcttggcga gcgtgatcaa
     5941 tctctccgaa tccccgcgaa tcgcggcggc cttggccttg gtcgtccgga ttcgttcgcg
     6001 acgaaatagc tcggtgatca ggttgcgcct cagcgcggtt cgattcccga tggaccgatt
     6061 gagtctgcga ccccctactc gatgcctcat ccctcggcca ccgccgtttc cggttcaagc
     6121 tctgccgacg tctcgtcgct gagatatccc ttctcccgca ggcgctcttt caattcatcc
     6181 aggctcttct ccccaaaatt tcggatcgag agcatggcgt cctgcccctt ctcgagcatg
     6241 tccagcactt cgcccacgct ggtgatcccc gtccgcttca aagagttgaa cacccggacc
     6301 gagagatcca agttctcgat cgggatctca tagacctcgc tagccatctt gggttcttct
     6361 tcgcgggtgg cggccgcgat caagctctct tcagtcacgc ctgccaggtc gcgcaagtgc
     6421 gcgatcagga tggtggcgct gcggctgaga gcctgctcgg gtcggagggt cccatcggtc
     6481 cagatctcca tcgtcagccg atcgaagctg gtatcctggg ccaccctggc ctggcccacc
     6541 tcgaagttca ccttgcggac cggaccaaat atcgcgtcgg tcggcagctc tccgatgggc
     6601 agcctggccc gctcgtcgga gggcgaatag cctcggccgc gctcaacatt cagctcgatt
     6661 tccagattgg ccttcgcgtc gtcaacagtg aagaggtaga gctcgggatt gacgatctcg
     6721 acctcggccg gggcgatgac gtcggccgcc gtcacaaccc cctctccgcg tacttcgagg
     6781 cggagtcggg cagtctcccc ttcgtgcaac acgagccgca gctgcttgaa gttcaacatg
     6841 gcttgaacca cgtcttcccg aaccccgggg acctcggaaa actcgtgttg aacgtcggag
     6901 aaccggaccg aagtgaccgc ggccccctcc agcgaggaaa gcaagacccg ccgcagggcg
     6961 ttcccaagcg tgatcccgta cccgctctcc agcgggctga taatgaatcg gccgtagttt
     7021 cgagcgattg cgtcgcgctc gattttcggc atcaccatgt tcatgctgag tggcactgcc
     7081 cccctccccg agacccaagt ctcactgtgc aattggtgtg gtttgtcatt ggaattaggc
     7141 tggtatgatc cgcaggcggc ggcccttacc tggagtagaa ctccacgatg agctgctcat
     7201 tcaggttggc gtcgatgtct ccccgttccg gattctgaat tactcgggcg ctcagactgg
     7261 tcgagtcccg ctgcagccat ttcggggcgg cccggctctc tgcggattga agtaggtttt
     7321 ggaagtaggt acgcttgcgc gagccctccc tgacttcgat cacgtctccc ggcttgatca
     7381 gcatagacgg aatatcggtc cgacgtccat tcaccgagaa gtgtccgtgg gtgaccaaca
     7441 gccgcccctg ggcccgactc tcggcgaagt tcagtcggta caccacgttg tccaatcggc
     7501 gttcgagcat ccccagcagg ttcaccccgg tcattccccg ggtctggagc gcctccgcat
     7561 agtagcggcg gaactggcgc tcggtcacgt tgtagactcg gcgggtcttc tgcttttccc
     7621 gcagctggcg ccaatactcc gaaacccggc gccgccgcca ttgggcctga cggccatggt
     7681 ctcccggtgg gtattcccgc cgctcgaacg cgcacttagg cgtgtaacag cgttgtcctt
     7741 tgaggaagag cttctcgccc tcgcgccgac acagtttgca cacagaaccg gtatatttcg
     7801 ccatgattcc tttccgctcg cctgttcgct gagttccgat cgcccgaaag gatcggctca
     7861 gactctccgc ttcttcgggg gtcgacaccc gttgtgaggg atgggggtga catcgctgat
     7921 cgaacgaacc ctcactccgg acgattggat cgcccggatc gccgattccc gccctggtcc
     7981 gggaccgttg acgaaaatgt ccacttcctg gagacccagg tctcgggccg accggatcgt
     8041 gacctccgcc gcggttctgg ccgcaaacgg ggtactcttg cgcgagccct tgaacccgcg
     8101 cgatccggcg ctgccccaaa ccactgcgtt accttgtgga tcggtgatgg tgatgatcgt
     8161 gttgtt
//
LOCUS       DATDPQ010000133         5675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1365392, whole genome shotgun sequence.
ACCESSION   DATDPQ010000133 DATDPQ010000000
VERSION     DATDPQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365392"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VI520_03200"
     CDS             <1..825
                     /locus_tag="VI520_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF36922.1"
                     /translation="FQTPTGILGTALLIFMGVAVGQLIDLRGKVAQDIEARRKVEDKL
                     GNLSRAREALVDGISHDLRSALHTISASLELLRSGKVKEAPQQYELLSWAALDAKRLI
                     ATVDDLEDLSSVESSGLRLSPEPLELNSLVAEVVENLRQYGESRGITLVHISAERALL
                     VQGDRLRLKRVLFRLAEHAITVSDVGSQVVILAESEKNGVAIEFIDHGPAEAAERLLK
                     LFEKPEDIGRAGEARSQKGMGLYVARLIVEAHGGKLAVENQTGPGNTLKFTIPIQA"
     gene            complement(893..2251)
                     /locus_tag="VI520_03205"
     CDS             complement(893..2251)
                     /locus_tag="VI520_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF36923.1"
                     /translation="MRLGIKLILGFLVVGLAGVALVALIASRATENEFGRFMFDRYHE
                     SVLTQLQDYYLIHGGWEGVDVVKPAPFFQLPIEPHRQALPPQGPITLANAQGIILVGG
                     PGFEPGMSVQPTTLPGATAVQVDNQVVGWFIASPEGFGRNPAEIVFLDRTNRLLVVGA
                     VGGGIVSLLLGVLLTRNLTRPIRELTAASRAIASGDLNQTVPVRSRDELGELAAAFNQ
                     MSSDLARARDLRRQMTADIAHELRTPLSIILGQIDALDDGVVPTSPESFEVLREETGR
                     LGRLVEDLRTLSMADAGELTLVRRLVQPGQLLEQAQAAHQPLAAAKGVSIRVRASSGL
                     PEAELDPDRLLQVLGNLLSNALRHAPSASEIELAAESREAGIQMTVRDNGPGIEAVDL
                     PHLFDRFYRTDKSRQRETGGSGLGLAIAKAIVELHGGRIWAESTPGQGTAILIWLPTA
                     PG"
     gene            complement(2258..2941)
                     /locus_tag="VI520_03210"
     CDS             complement(2258..2941)
                     /locus_tag="VI520_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36924.1"
                     /translation="MTKTILVVDDEPRLVSLVEAYLSQEGFKVLSAGDGRDALFLARQ
                     EKPDLIVLDIMMPEMDGYEFMRLHRKERETPIILLTAKVEADDRVLGLELGADDYVTK
                     PFRPRELVARIRAVLRRAGQTQPSAQKLRAADVSLDRETHTVEVNSQFVDLTPSEFDL
                     LAALMSVPGRAYTRLELLDRIQGVAYEGYERTIDVHIKNLRAKLEPDSRNPRYVQTVY
                     GVGYRFARE"
     gene            complement(3041..3757)
                     /locus_tag="VI520_03215"
     CDS             complement(3041..3757)
                     /locus_tag="VI520_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLF36925.1"
                     /translation="MASVCEIWSKWNRVWLLQLVLLSGCAAPTAGDPTALLSDLPADS
                     AADTLPSSEPPAGEPAEFVTFRIDPSASQARFVIGEILGGAPNTVIGVNDQVSGEILI
                     SASNPADSQIGPIEIGAASFVTDNALRNRAINQFVLQSGIYPLITFTPTSIGGIPGEV
                     SPGAVFTLQIAGDLTIREVTHPVSFEATVTVVSESEIQGNATAAISRSDFELTIPSVP
                     RVAGVDEQLVLELDFVALAE"
     gene            complement(3825..4538)
                     /locus_tag="VI520_03220"
     CDS             complement(3825..4538)
                     /locus_tag="VI520_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36926.1"
                     /translation="MTKFWRFSFSVGALVVLAGCSPAPAAGSILTEDYEGALPANVQL
                     ILGTLQLEGTDLAVTAEQASSLAPLWKAVRSLSASDTAAEAELEAVLDQIQEGMASDQ
                     LEAIAAMELNQEDLFTTVQEMGIQPFAPSAAGRFLGSGTGDFIGGGSEGGPPGGGGEG
                     GGPTFFIGPAEGPPGGTAPGGFVEDQLSEEFTSNLDPDQIETLEAQREAGGGPGARIA
                     PFLLDPLIELLESRAGIVP"
     gene            complement(4587..>5675)
                     /locus_tag="VI520_03225"
     CDS             complement(4587..>5675)
                     /locus_tag="VI520_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36927.1"
                     /translation="GSNLLFVQSGFVRFGPGGRSVESAAPAGQAGGGLVYDDAFAIAE
                     QVDGVVAVAVEQSSVQTVEVGSVSLDSVSIVGSTADYPSVRDMAVENGRYFTQQEVDR
                     KQKVAVLGQSLAEELFGDADPIGQTVNVGGVKLRVIGVFEERGLVSGVDFDSQLYIPI
                     SVVFQKFTPSLFARIIGDRVRMIYVEVESPESVESAIQQIGLLLAKRHSVSLEEADFT
                     ITTQQDIIETQEATTAAFRNLLAWVAGVSLIVGGIGIMNIMLVSVTERTREIGIRQAI
                     GAAPTDIRWQFLTEAVVLSLAGGVIGVLVGVAGSWVFGAVSQLRTVILGSSILLAFGS
                     AAAVGIFFGFYPANRAAQLDPIQALRHE"
BASE COUNT         1066 a         1850 c         1759 g         1000 t
ORIGIN      
        1 ttccagacgc cgaccggcat tctcggcacc gcgctgctga tctttatggg cgtggcagtc
       61 ggccagctga tcgatctacg gggtaaggtt gcccaagata tcgaggcccg gcgcaaggta
      121 gaggacaaac tcgggaatct gagccgggct cgcgaggcgc tggtggatgg aatctcgcat
      181 gacctgcgga gcgcgttgca cacgatctcc gcctcgctcg agctgctccg ctccgggaaa
      241 gtgaaagaag ctccccagca gtacgagctg ctgtcctggg cggcgctgga tgccaaacgg
      301 ctgatcgcga ccgtcgatga tctggaagat ctctccagcg tggagagcag cgggctgcgg
      361 ctcagccccg agccgctgga actcaactca ttggtcgccg aggtggtcga gaaccttcgc
      421 cagtatggtg agtccagagg catcaccctg gtccacatca gcgccgagcg ggcgttgtta
      481 gttcagggcg atcggctgcg gctgaagcgg gtcctgttcc ggctggcgga gcacgccatc
      541 acggtctccg acgtcggatc ccaggtggtc attcttgccg aatccgaaaa gaatggggtc
      601 gcaatcgagt tcatcgatca cggcccggcc gaagctgcgg agcggctcct gaagttgttt
      661 gaaaagcctg aggacattgg gcgcgccgga gaggcccgct cacaaaaggg aatgggactc
      721 tatgttgccc ggctgatcgt cgaggcccat ggcggcaagc tcgcggtcga aaaccagact
      781 ggcccaggga acacgctgaa gttcacgatc cccattcagg cctgacccac tcgccctcag
      841 gaagctcctt ccagttcaca actcgaacgt agtcgtctgc caggcccggc actcagccgg
      901 gggccgtcgg cagccagatc aatatcgcgg tcccttggcc tggagtcgat tcggcccaga
      961 tccgcccgcc gtgcagttcg acgatcgctt tggcaatggc cagtccgagc ccggagcctc
     1021 cggtctcacg ctgccgggac ttgtcggtcc ggtagaagcg gtcgaacagg tggggcaggt
     1081 cgacagcctc gattcccgga ccgttgtcac gaacggtcat ctgaatcccg gcctctcggc
     1141 tctccgccgc gagctcgatc tcgctggcgg acggcgcgtg gcgcaaggca ttgctcagca
     1201 gatttccaag cacctgcagc aggcgatccg gatcgagctc agcctccggc agaccggacg
     1261 aggcccgcac ccgaatcgag acacccttcg ctgcggcgag cggctggtgg gcggcctggg
     1321 cctgctcgag cagctgcccg ggctgcacga gtcggcgcac cagggtcagc tcgcccgcgt
     1381 ccgccatcga cagggtccgc agatcttcaa cgagccggcc cagccggccg gtctcctcgc
     1441 gcagcacctc aaaggactcg ggagaggtgg ggactacccc gtcgtccagg gcatcgattt
     1501 ggcccaggat gatgctcagc ggtgtgcgca actcgtgggc gatgtcggcg gtcatctggc
     1561 gtcggaggtc gcgggcgcgg gcgaggtcgg aactcatctg gttgaaggcg gctgccagct
     1621 cgcccaactc gtcccgcgag cgcaccggga cggtttggtt gaggtcgccg gaggcgatcg
     1681 cccgcgaggc tgcggtcaac tcccgaatcg gccgggtcag gtttcgggtc agcagcactc
     1741 ccaacagcag cgagacgatg ccgccgccga ccgccccaac gaccaacagg cggttggtgc
     1801 gatccaagaa cacgatctcg gccgggttgc ggccgaagcc ctcaggcgag gcaataaacc
     1861 aacctacgac ctgattgtcg acctggactg cggtggctcc aggcagagtc gttggctgga
     1921 cactcatccc cggctcgaag ccaggaccgc caacgagaat aatcccctgg gcattcgcca
     1981 gcgtgatcgg tccctggggc gggagagcct gacggtgcgg ctcgatcggc aactggaaga
     2041 acggagccgg cttgaccacg tcgaccccct cccagccccc gtggattaga tagtagtcct
     2101 ggagctgggt gagcactgac tcgtgatacc ggtcaaacat gaagcgacca aactcgttct
     2161 cggtcgcccg actagcgatg agggccacca gggccactcc ggccaggccg acaaccagaa
     2221 accccagaat cagcttaata cccagtcgca tcggtgctca ctcgcgggcg aaccggtagc
     2281 caactccata gacggtctgc acatagcgcg gatttcggga gtctggctcg agttttgccc
     2341 gcaggttctt gatatgcacg tcaattgttc gttcgtagcc ttcgtaagcc actccttgaa
     2401 tccgatccaa taactcgagc cgggtgtagg cccggccggg gaccgacatc agggcggcca
     2461 gcaaatcgaa ttcagaggga gtcaaatcga cgaactgact gttgacctcg accgtatgag
     2521 tttcccgatc gaggctgacg tctgccgccc gcagcttttg ggcgctgggc tgagtttggc
     2581 cggcccgccg caggacggcc cgaatccggg ccaccaactc ccgcggccga aacggtttcg
     2641 tcacatagtc gtctgccccg agctccagtc cgaggactcg gtcgtcggct tcgaccttgg
     2701 cggtcagcaa gatgatgggg gtctctcgct ccttgcgatg cagccgcatg aactcgtagc
     2761 cgtccatctc gggcatcatg atgtccagca cgatcaggtc cggcttctcc tggcgggcga
     2821 ggaacagcgc gtcccgccca tcgccggccg agagcacttt gaagccctcc tgggagaggt
     2881 aggcttctac cagcgagacc agtcgcggtt cgtcgtcgac aaccaggatg gtctttgtca
     2941 ccactccccc ttttcgtttg ggtctgagta gatcctggcc aatcgtagca agctcgtgtg
     3001 aagatactgt gaaggtttca agaattccag gtgagctcgg ctactcggcg agtgcgacga
     3061 agtcgagttc cagcaccaat tgctcgtcaa ctcccgccac ccgggggacc gaagggatcg
     3121 tgagctcgaa gtcggagcgg gagatggctg cggtggcatt cccctggatc tcgctctcgc
     3181 tgactacggt gaccgtcgcc tcgaagctca ctgggtgggt gacctcccgg atcgtcagat
     3241 ctccagcgat ttgaagcgta aagaccgcac cagggctgac ctcgcccgga atgccgccaa
     3301 ttgatgtcgg cgtgaaggta atgagcggat agatcccgga ttggaggacg aactgattga
     3361 tcgcccggtt ccgtaaagca ttgtcggtca caaagctggc ggcgccgatc tcaatgggac
     3421 cgatttggct gtcggccgga ttcgaggcgc tgatcaggat ctcgccgctg acttgatcgt
     3481 tcactccgat tacggtgttg ggtgcgccgc cgaggatctc gccgatcacg aagcgggcct
     3541 ggctcgccga cggatcgatc cggaaggtca cgaactcagc tggctcgccg gccggtggtt
     3601 cggacgaggg cagtgtatcc gcggcactgt ccgccggcag gtcgctcagg agggcagtgg
     3661 gatcgcccgc cgtcggtgcc gcacagccag acaggagcac tagctgcagc agccatactc
     3721 ggttccattt gctccagatt tcacaaacac tggccatagg gctccttgaa accactttcc
     3781 gattgtaccc atccactcgg ctaaccggtt cagcctcccc cggcttaggg gacgatgccg
     3841 gcccgagatt caagcagctc gatcagcggg tccagcagga agggtgcgat gcgggcacct
     3901 ggcccgccgc cggcctcccg ctgggcctcg agcgtctcga tctggtccgg atccagattg
     3961 ctggtgaact cttcggaaag ctggtcctcg acgaacccac ctggagctgt gcctccgggc
     4021 ggaccttctg ccggcccgat gaagaaggtc gggcctcctc cctccccgcc gccgccgggc
     4081 ggcccaccct cggatccgcc cccgatgaag tccccggtcc ccgatcctaa gaatctcccc
     4141 gcagcgctcg gggcgaacgg ctggataccc atttcctgaa ctgtggtgaa cagatcttcc
     4201 tgattaagct ccatcgccgc gatggcctcg agctgatcgc tggccatgcc ctcctggatc
     4261 tggtcgagga ccgcctcgag ctccgcttcg gccgcggtgt cgctggcgct caggctgcgg
     4321 acggccttcc acaagggagc cagagagctc gcctgctcgg cggtgacggc gagatcggtc
     4381 ccttccagct gtaaggtgcc caagatcagc tggacatttg ccggcagggc accttcatag
     4441 tcttcagtca atatcgagcc agccgcgggc gccgggctgc agccggctag cacaaccagg
     4501 gcacctaccg agaagctaaa cctccagaac ttggtcacca aacacctccg ggtcgaaacc
     4561 ctctttctga acatggatcg aacggctcac tcatgccgca aggcttgaat tggatcgagc
     4621 tgggcagccc ggttcgccgg atagaagcca aaaaagatgc ccaccgcggc ggcggagccg
     4681 aaggccaaca ggatcgaact ccccaggatc acggtgcgta gctggctgac cgcgccaaag
     4741 acccacgacc ctgcgacgcc gaccaggacc ccgatcactc cgccggccag actcagcacg
     4801 accgcctcgg tcagaaactg ccagcggatg tcggtcggcg cggcaccgat cgcctgtcga
     4861 atcccaattt cgcgggtccg ctcggtgacg ctcaccagca tgatgttcat gatcccgatc
     4921 ccgccgacga tcaaagagac ccccgcgacc caggccagca ggttgcggaa ggcggccgtg
     4981 gtcgcttcct gggtctcgat aatgtcctgc tgggtggtga ttgtgaaatc ggcctcttcc
     5041 aagctgaccg agtggcgctt cgccagcagc aatccgatct gctggatcgc gctctccacg
     5101 ctctccgggg actccacttc cacatagatc attcgcaccc ggtcgccgat aatgcgggcg
     5161 aacaggctgg gggtgaactt ctgaaagacg accgagatcg ggatatagag ctgcgagtca
     5221 aaatccaccc cgctcaccag cccgcgctct tcgaagaccc cgatcacccg cagcttcaca
     5281 ccgccaacgt taaccgtctg gccgatcgga tctgcgtctc cgaagagctc ctcggccagg
     5341 ctctgcccga gcaccgcgac cttttgtttc cgatcgacct cttgctgggt gaaatagcgg
     5401 ccgttttcga cagccatgtc ccggacggag gggtaatcgg ccgtgctgcc gacgatcgag
     5461 accgaatcca gcgacacgct gccgacttct acggtctgga ccgaggactg ctcgaccgcc
     5521 accgcgacca caccatcgac ctgctcggcg atggcgaagg catcgtcgta gaccaacccg
     5581 cccccggcct gcccggctgg cgcggcggac tcaacggaac ggccccccgg cccaaatcgg
     5641 acgaatccgg actgaacgaa gagcaggttc gagcc
//
LOCUS       DATDPQ010000134         3191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3449749, whole genome shotgun sequence.
ACCESSION   DATDPQ010000134 DATDPQ010000000
VERSION     DATDPQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449749"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VI520_03230"
     CDS             complement(<1..912)
                     /locus_tag="VI520_03230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HLF36928.1"
                     /translation="MKNLTVVGVGHVGLVTGACFADLGNRVVALDTDQERIAGLQRGQ
                     LPIYEPGLEELVGRNLAAGRLRFTTSYSEAVAESEFVFIAVGTPGGAEGEADLKHIRQ
                     AAESIAQEMIAPLIIVNKSTVPVGTGDWLADIVRSNQRVSIPFSVVSNPEFLREGAAI
                     TDFMHPARTVLGSTDPRAAEKVAQLHLPLRAPIMITDLRTAEMIKYASNAFLATKISF
                     INEIANICEALGADIKEVATGMGYDPRITPHFLEAGLGYGGSCFPKDVKALAYMAAEK
                     GRHPQLLQAVMEINDDRRALLVDKTREM"
     gene            1010..1537
                     /locus_tag="VI520_03235"
     CDS             1010..1537
                     /locus_tag="VI520_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLF36929.1"
                     /translation="MEVSQAIRMKRAVRSFTPQPLPEPALRSILNAGRRAQSAKNRQA
                     WHFLVIRDRQSLEGLSKLGKYAGHLAGAAAAIGILTPDPAERWSIMFDAGQAAAYMQL
                     AAWELGIGSCPATIYLPEEARELLGVPADLHLRVVFSFGYPADPAELTAPPQQGGRKT
                     LGEVVSFEHWQDPSR"
     gene            1564..2685
                     /locus_tag="VI520_03240"
     CDS             1564..2685
                     /locus_tag="VI520_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF36930.1"
                     /translation="MLTRTVINTGYRMVYPFLPVIARGLGVAPEAIILGVTGRQALGL
                     ATPLLGALADRVGRKPAILAGVAIFILGGLIVGLHPSYTTLVLALIAVGLSKSLFDPA
                     LYAYVGDQVVFERRGTPMALVELSWSGAYLLGVPAVGLLIGAAGWVAPFPALAAMALL
                     GALWIGFTLPGNAGSAVRAASITSGFAAILTRKSALAGLAVGFLISAGNETVSIVYGL
                     WMEQSFGLRIAALGAATAVIGLAELGGEGLVAGLADRIGKRRLVAAGMVMNTAAALVL
                     PRLSGSLAVGLIGLFAFYLCFEATIVCTLPLMSEQVPQARATLLATNSAGLSLGRSLG
                     ALAGAPLFSLGMQANGAAAAALNLLALGLLLGLVKERGS"
     gene            2688..>3191
                     /locus_tag="VI520_03245"
     CDS             2688..>3191
                     /locus_tag="VI520_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HLF36931.1"
                     /translation="MEQRPAIDLLVHSASQLLTLAGGPQRGTALGALGIIDNGAVAIR
                     GELIVATGSTEDLRRSYVAANELSAAGRVVLPGFVDPHTHLVWAGDRAAEFEQRVQGA
                     SYLEILEAGGGILSTVRRTRFASLETLLSESRPRLRQMLEHGTTTAEAKTGYGLELEA
                     ELRLLQAI"
BASE COUNT          514 a         1034 c         1038 g          605 t
ORIGIN      
        1 catttcgcga gttttgtcga ccagcagcgc tcgccggtcg tcgttgattt ccatcaccgc
       61 ctgaagcagc tgcggatgac gccccttttc cgccgccatg taggccaggg ccttgacgtc
      121 tttgggaaag cacgaccctc cgtagcccaa cccggcctcc aggaaatgag gagtaatccg
      181 ggggtcgtac cccattccgg tggccacttc cttgatgtcc gcgccgagcg cctcacagat
      241 gttggcgatc tcgttgatga aagagatctt ggtcgccaga aacgcgttcg aggcgtactt
      301 gatcatctcg gccgtgcgca aatcggtaat catgatcggg gcccgcaatg gcaagtgaag
      361 ctgggctact ttctcggcag cccgcggatc ggtagagccc aggaccgtgc gagccgggtg
      421 catgaagtcg gtgatcgccg ccccttcccg taggaactcg ggattcgata cgaccgagaa
      481 tgggatagaa acccgttggt tggatcggac gatgtccgcc aaccagtctc cggtgccgac
      541 cgggacggtc gatttgttga caataatgag cggcgcgatc atttcctggg caatcgattc
      601 agcggcctgg cgaatgtgct tgaggtcggc ttccccttct gctcccccgg gggtccctac
      661 tgcgatgaag acaaactcgg actctgcgac ggcctccgaa taggaggtgg tgaagcgcag
      721 ccgaccggcc gcaaggttgc gcccgaccag ctcttccaac cccggctcgt agattggcag
      781 ctgtccgcgt tgcagcccag caatgcgctc ctgatcggtg tccagggcca cgacccggtt
      841 cccgagatcg gcaaagcacg ctccggtcac caggccgaca tggccaaccc cgaccaccgt
      901 caaattcttc aaaaagctgt ctcctaaggg gggcggcaat aataccatgc aaccccggcc
      961 atgagcctag ctggcgtaaa atcaacggga tcccaccatg gagggtgccg tggaagtcag
     1021 ccaggcaatt cgaatgaaaa gggcggttcg cagtttcact ccccagccgc tgccggagcc
     1081 ggcgcttcga tccatcctga acgccggccg tagggcccaa tcggccaaga accgccaggc
     1141 atggcatttt ctcgtcatcc gcgaccgcca gtccctggag ggattgtcca agctgggcaa
     1201 gtacgccggc cacctggcgg gggcggcggc cgccattggc attctcaccc ctgacccggc
     1261 tgaacgctgg tcgatcatgt tcgatgccgg ccaggccgcc gcctacatgc agttggcagc
     1321 ctgggaactg gggattggat cctgccctgc gacgatctac ctgccggaag aggcccgaga
     1381 actgctgggg gtcccggccg acctacattt gcgggtggtg ttttcgttcg gttacccggc
     1441 agacccggcg gagctcacag cgccacccca gcagggcggc cgcaagacgc tgggggaggt
     1501 cgtctctttc gagcactggc aggacccctc ccgctgagat tctcccgcca gctggtcccc
     1561 ctgatcctta cccggaccgt catcaacacc ggctatcgga tggtctatcc attcctgccg
     1621 gttatcgccc gcggcctcgg cgtggctcca gaagccatta tcctgggggt gaccggccgc
     1681 caggcgctcg gcctggcgac cccgctgctg ggagccctcg ccgatcgagt cgggcggaag
     1741 ccggccatcc tggcgggcgt agctatcttc atcctcggcg gattgatcgt gggcctgcac
     1801 ccgagctaca ccacgttggt cctggccttg attgcggtcg gcctctcaaa gtcactgttc
     1861 gacccggccc tgtatgccta tgtgggcgac caggtcgtct tcgagcgccg ggggactccc
     1921 atggcgctgg ttgagctgag ctggtctggc gcatacctgc ttggggtacc ggcggtcggc
     1981 ctgctgatcg gtgcggccgg atgggtcgcc ccgttcccgg ctttggcagc catggcgctc
     2041 ctgggggcgc tctggatcgg gttcactctt cctgggaacg ccggctccgc ggtgcgggcc
     2101 gcctcaatca cgagcggctt cgcggccatc ctcaccagga aatctgccct cgccgggctg
     2161 gcggtgggct ttctgatttc ggccggcaat gagacggtca gcatcgtgta cggactttgg
     2221 atggagcagt ccttcggcct gcggatcgcc gccttggggg cggctacagc cgtgatcggt
     2281 cttgccgagc tcggcggcga agggctggtc gcgggcctgg cggatcggat cgggaagcgc
     2341 cggctggtcg cggccggaat ggtgatgaac accgcggccg ccctcgtgct tccgcgactt
     2401 agcggatcgc tcgcggttgg actgatcggc ttattcgcgt tctacctttg ctttgaggcc
     2461 acgatcgtct gcaccttgcc gctgatgagc gagcaggtcc cgcaggcccg ggcgaccctg
     2521 ctggcgacca acagcgccgg cctgtcgctg ggccgcagtc tgggggctct cgccggagct
     2581 ccgctgttct cgctgggcat gcaggcgaac ggagccgccg cggcggcgct caatctgctg
     2641 gcgctggggt tgttgcttgg cttggtcaag gagcgaggct cttgaatatg gaacaacgac
     2701 cggcaattga tctgcttgtc cactccgctt cccagctgct caccctcgcc ggcggcccgc
     2761 agcgtgggac tgccctaggc gcactcggaa tcatcgacaa cggtgcagtt gccattcggg
     2821 gcgagctcat cgtcgcgacc ggctcaaccg aggatctccg ccggtcgtac gttgcagcca
     2881 acgagctgag cgccgccggc cgggtggtcc tgccgggttt tgtcgacccg cacactcatc
     2941 tggtgtgggc cggcgaccgc gccgccgaat ttgaacaacg ggtccaggga gcgagctacc
     3001 tggaaattct ggaggccgga ggtggcatcc tctcgacggt gcgccggacg cgattcgcca
     3061 gcctcgagac cctgctttcg gagagccgcc cgcggctgcg gcagatgttg gagcacggaa
     3121 cgacgaccgc cgaggccaag accgggtacg ggctggaact cgaggccgag cttcgtctgc
     3181 tgcaggcaat c
//
LOCUS       DATDPQ010000135         3814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2666113, whole genome shotgun sequence.
ACCESSION   DATDPQ010000135 DATDPQ010000000
VERSION     DATDPQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666113"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1491)
                     /locus_tag="VI520_03250"
     CDS             complement(106..1491)
                     /locus_tag="VI520_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HLF36932.1"
                     /translation="MINRRIGALLGGLGLLLAGCAQATPAAPTVEALPTAGEAGLTPS
                     VTVSDQDASGGTVTIAEVVAAEAGWLVIHTTKSGAPGPVIGWTQVLTGTSEGLPVEID
                     LARATGQLFAMLHVDAGYQGEYEFPGPDAPVTAADQLVNVPFNATFPIEPSVTVSDQD
                     ASEGNVSIESVMAAAPGWLVIHISQGGAPGPVIGQSPVIVGYNPEVGVAIDLSQATGQ
                     LFAMLHLDVGAAGVYEFPGADAPVFAGDAPVNVPFNATFAITPSVTVSNQLVAEGTVT
                     IDKVSAAEAGWIAIHTDASGSPGPVIGFAPVGIGHNQNVKVEIDEAQATPILYAMLHV
                     DAGVLGEYNFPGDDGPIFDAAGDVVMTPFQLQAEEASAGPVTVLVVDVNFREKEVTVP
                     VGTTVTWMYNGNLPHTVTSDDGLFESGTLSSGGSFSYTFDEAGTFPYHCRFHGGPGGS
                     AMAGVITVEAG"
     gene            complement(1579..2115)
                     /locus_tag="VI520_03255"
     CDS             complement(1579..2115)
                     /locus_tag="VI520_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36933.1"
                     /translation="MLTACAGRPATSVELAGSATPDQGANLSATATRPAKSIASPPTA
                     PTENPSQLYVILDGEPTPIENGESVQLTDVLGVQITVDPYPPMGLSAQPVLDLYLFDR
                     AGQPVHDAAIEIFYDMLSMPHGPFQAASREQERGHYLTSLDLIMFGNWGMEIEILPAD
                     GAALVRLRIVLIVELPEE"
     gene            complement(2157..2924)
                     /locus_tag="VI520_03260"
     CDS             complement(2157..2924)
                     /locus_tag="VI520_03260"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HLF36934.1"
                     /translation="MEGEERPMAHKEQQLLGAYVMGALDSDERIELEAHLGTCQECQA
                     ELVEYQQVTEGLLHLATPVAPSSGLRAGLTARLAGHPGTPLSPRRPGLIRGPQLGFAA
                     LVVALLVLNGALILETRRLAERGQELLAQQEASQTALALASYSTSQTAFVQEESIWGT
                     FVYEGNFPLAVFNVWGLPTLPEDQAYQLWLFNPDGTRSTGALFRTSTSQDLISVLIRS
                     PVPLGEVLGFGVTVEPNTGSPAPTGPRIIGADFDHSP"
     gene            complement(2927..3502)
                     /locus_tag="VI520_03265"
     CDS             complement(2927..3502)
                     /locus_tag="VI520_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF36935.1"
                     /translation="MNGTSLPDDATLIQQVAQGDRRAFLSLYDKYAVRVHGLSLRMLR
                     DAMSAEEVTQDAFLRLWTRAQTFNPDRGKVLSWLLAITRRLALDRFRLDSRAPPPSES
                     DNPGEDLEGLPDESSRTEEARWRSLRFALADLPVEQRLPIELAFYHGLSQREIAEHLR
                     IPLGTVKTRVRLGMDKLRRAWATDPIQSGRR"
BASE COUNT          714 a         1200 c         1224 g          676 t
ORIGIN      
        1 cggcgtcgat cagtgccggt tcgtccacgg tcggtcatga taacccaaag aaaacaacag
       61 gggccagcta agctggcccc tgcgaaactc acggggttgc ttgactcaac ctgcttctac
      121 ggtgattacc ccggccatcg cggagcctcc cggcccgccg tggaagcggc agtggtaagg
      181 gaaggtccca gcctcatcga acgtgtagct aaagctcccg ccgctgctga gggtcccact
      241 ttcgaagagg ccatcgtcag aggtcaccgt gtggggcagg ttgccgttgt acatccaggt
      301 gacggtcgtt ccgaccggga cggtgacctc cttttcacgg aagttcacat ccaccaccaa
      361 cacagtgacc ggtccggcac tggcttcttc cgcctgaagc tggaacgggg tcatgacgac
      421 atcgcccgcg gcgtcaaaga tggggccgtc atcgcctggg aagttgtact cgccgagcac
      481 cccggcgtcc acgtgcagca tggcgtagag aatgggcgtc gcttgcgcct cgtcgatctc
      541 aaccttgacg ttctgattat gtccgatacc gactggcgcg aagccgatca ccggtccggg
      601 ggacccgctg gcgtcagtat ggatggcgat ccagccggcc tcggctgcgc tcaccttgtc
      661 gatcgtcacg gtcccttcgg ccaccagttg attgctgacg gtcacggacg gggtgatggc
      721 gaaggtcgcg ttgaacggta cattgaccgg cgcgtcgcct gcgaacaccg gagcatccgc
      781 tcccggaaac tcgtagaccc cggctgcccc cacgtcgagg tgcagcatgg caaagagctg
      841 gccggtcgcc tgactcaaat cgatggcgac acccacttcg gggttgtaac caacgatcac
      901 cggggattgt ccgatcaccg ggccgggggc acccccctgg ctgatgtgga tgaccagcca
      961 gcccggcgca gccgccatca ccgactcgat gctcacattc ccctcgctag catcctgatc
     1021 gctcacggtt accgaaggct caattggaaa cgtggcattg aagggaacat tgacgagttg
     1081 atcggcggct gttacgggcg catcggggcc ggggaactcg tactcgccct ggtagccggc
     1141 atccacatgc agcatggcga agagctgccc ggtggctcgc gccagatcga tttcgacggg
     1201 taggccttcg ctcgtcccag tcaatacctg ggtccaaccg atcaccggac ccggggcccc
     1261 actcttggtg gtgtggatga ccaaccaacc ggcttcggcg gcgacgacct cagcgatcgt
     1321 gaccgttcca ccgctggcgt cctgatcgct caccgtgacc gatggggtga gcccggcctc
     1381 gccggccgtc ggcagggcct cgaccgtcgg cgctgcaggt gtcgcctggg cgcaacccgc
     1441 caacagcagc cccaggccgc caagtaaagc tccaatcctt ctattaatca tcactcctcc
     1501 tttaagttgt ttgtggcagt tcggtccggt aatcaatacg aatgtggcag gccggctgga
     1561 tctgtcttaa tcaccctctc actcctcggg cagctcgaca atgagcacga ttctgagccg
     1621 gaccagcgcg gcgccgtcgg ccgggagaat ctcgatctcc atcccccaat tgccaaacat
     1681 gatcagatcc aaggaggtca ggtagtgccc gcgctcctgc tcacggctag ccgcctggaa
     1741 tgggccatgg ggcatcgata gcatgtcgta aaagatctcg attgccgcgt cgtgcaccgg
     1801 ctgcccggcc cgatcaaaaa ggtagagatc gagtacgggt tgagcggata agcccatcgg
     1861 agggtacgga tcgaccgtga tctggacccc aagcacatcg gtcagctgca ccgattctcc
     1921 attctcgatc ggggtcggct ccccatccag gatcacgtac agctgtgagg ggttctcggt
     1981 cggggctgtg ggtggcgagg caatcgattt agcgggccgg gtcgcagtcg ccgagagatt
     2041 ggcgccctga tccggggtgg cagaaccagc aagttcgact gaagttgccg gacggccggc
     2101 gcaggcagtc agcaacagga taagtgccag ccggctactc tggatctgga gcggcctcag
     2161 ggtgagtgat caaaatcggc cccgataatg cgcgggccgg tcggcgccgg gctgccggta
     2221 ttgggctcga cggtgacccc gaatcccaac acttctccga gcggtacggg tgagcggatc
     2281 agcacggaga tcaggtcctg ggaggtgctg gtcctaaaca gtgcccccgt tgagcgggtg
     2341 ccgtccgggt tgaatagcca cagctgatag gcctgatcct caggcaaggt cggcagtccc
     2401 cagacgttga agaccgccag cgggaagttg ccttcgtaca cgaaggtgcc ccagatactt
     2461 tcttcctgca cgaacgcggt ctgggaagtg gagtagctgg ccagggccaa ggcggtctga
     2521 ctggcctctt gctgagccag cagctcctgc ccccgctcgg ccagcctgcg agtctccagg
     2581 atgagggcac cgttgaggac cagcagcgcg accaccaggg ccgcgaagcc aagctgtgga
     2641 cctcggatga ggcctgggcg gcgaggcgat agtggggtgc cggggtgccc ggcgaggcga
     2701 gcggttaggc cggccctcag gcccgaggaa ggagcgaccg gggtcgccag gtgcagcagt
     2761 ccttccgtga cctgctgata ttcaaccagc tcggcctggc actcttggca ggtgccgagg
     2821 tgagcttcca gctcgatccg ctcgtcggaa tccaaagccc ccatgacgta cgcgccgagc
     2881 agttgttgct ccttgtgcgc catcggccgt tcttctcctt ccattattaa cgccggcccg
     2941 actgaatcgg atctgtcgcc caggcccgac gcagtttgtc cattccgagc cgaacgcggg
     3001 tcttaacagt cccgagcggg attcggaggt gttccgcgat ctctcgctga gacagcccgt
     3061 ggtagaaggc caattcgatg gggaggcgct gttcgacggg aagatcggcc aaggcgaagc
     3121 gcagactccg ccagcgggct tcttcggtcc ggctggattc atctgggagt ccttccagat
     3181 cctcaccggg attgtcactc tccgagggag gcggagcccg agagtccaga cgaaatcggt
     3241 ctagcgccag ccgccgggtg atggccagca gccaggagag caccttaccc cggtccgggt
     3301 tgaaggtctg ggcccgggtc cacaaccgga ggaacgcgtc ctgggtcact tcctcggcgg
     3361 acatcgcatc tcgcagcatc cgcaatgaaa ggccgtggac tcggacggcg tatttgtcgt
     3421 atagagacaa gaaggcccgc cgatcaccct gagcgacctg ctggatcagg gtggcatcgt
     3481 cgggcagact ggttccgttc acgccgggca ggattatacc cgccgtatgc cggtggctca
     3541 cccctcgggc ctgtgcgccc gtattgcgaa cggtacggct caaaaagaaa gacccgccgc
     3601 gcagcccttc agggcgacgg cgggcgggtc ttaaccgatg gcgggcgggc gagactactt
     3661 ccaggtagcg aagacgcccc ccatgacgcc gtgcacgagg agctggtagg ccgccagcaa
     3721 taaccaggca cccaccggcg gtccagagaa ggtcgtgaaa acgaaggtgg ccgtggcgcc
     3781 cagcccgatg aaggccactg ccccggccac gagt
//
LOCUS       DATDPQ010000136         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1629355, whole genome shotgun sequence.
ACCESSION   DATDPQ010000136 DATDPQ010000000
VERSION     DATDPQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629355"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VI520_03270"
     CDS             complement(<1..398)
                     /locus_tag="VI520_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36936.1"
                     /translation="MEQSDKTAAEAMGAEPERPGAAVKPTSRLGRFLRRALRWATAIL
                     VVFGLGVAATWFNQVRPRIAQQEALEQGLAAVEAQRDQLQAAVDELQGVQAENEVLQD
                     ELQETEGRLALLRVLIDVTSAQLGIAQEDP"
     gene            complement(411..1100)
                     /locus_tag="VI520_03275"
     CDS             complement(411..1100)
                     /locus_tag="VI520_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36937.1"
                     /translation="MTTGQAEPTAGSDERPQPALLARVSKVLLRLALVVLLGVALGAG
                     AYFGVTLAIRQLGEPTRQNSARIAALEAQLPEAVSRLAEQQESVAELQVQLEDLQAQL
                     GDQGRGQDQLGSQLTALADQLEDLTKRIEENTQAHQLRGDLQLTQAMLSLVRARLWLV
                     ENNLGLAIAEVERARELLQPLAAEQPEGSNLQAAIERLDFALIELNTRPLVAADDIEI
                     AWKLLVEPDEG"
     gene            complement(1081..1839)
                     /locus_tag="VI520_03280"
     CDS             complement(1081..1839)
                     /locus_tag="VI520_03280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLF36938.1"
                     /translation="MGYNGRRSPPICFPPQPEDPSLFPIRDAVRSRRFPVVNYALIAA
                     NVLSFLFVTSLGPSGYERFLGEFGVVPARLSFEQPLAGLTLFSSMFLHGGWFHLLSNM
                     WTLFIFGDNVEDQMGSGRYLGFYLIAGTVAGLAHAVSSPNSTIPTVGASGAIAGVLGA
                     YFILFPRARVTTFIPLFFLPWFVDVPAIVFIGIWFFTQLSQGLLSLGAIGDFGGIAWW
                     AHVGGFVVGLLLVRAFVARPKTPQVWVPDDHWSG"
     gene            complement(1855..>2040)
                     /locus_tag="VI520_03285"
     CDS             complement(1855..>2040)
                     /locus_tag="VI520_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HLF36939.1"
                     /translation="ASAGEALISELDRSGGFYRRIQLPEVIDAEAVEAKIENGLLTVR
                     VPKAEEARPKKIEVKAK"
BASE COUNT          406 a          684 c          588 g          362 t
ORIGIN      
        1 atcgggtctt cctgggcaat tccgagctgg gcgctggtca catcgatcaa cacccgcagc
       61 agggccagtc ggccttcggt ttcctgcagc tcgtcctgaa gcacctcgtt ctcagcctgg
      121 actccctgta gttcatccac cgcggcctgg agctggtctc gttgcgcttc caccgccgcc
      181 aagccctgct ccagcgcctc ttgttgggcg atccgcggcc gaacctgatt gaaccaggtc
      241 gccgccactc caaggccaaa gaccactaag atcgcagtcg cccagcgcag tgctcggcgc
      301 agaaagcgac ccaagcggct cgtgggcttc acggcggctc ccggtcgttc gggctcggcc
      361 cccatcgctt cagcggcagt cttatccgat tgttccatct gcggttctcc ttagccctcg
      421 tccggctcga ccagcagctt ccaggcgatt tcgatgtcgt cggccgcaac cagcggccgg
      481 gtgttcagct cgatcagtgc aaagtccagt cgctcgatgg cggcctggag gttacttccc
      541 tccggctgtt cggccgccag gggttgcagc aattcccggg cccgctccac ctcggcaatc
      601 gccagcccga ggttattctc caccaaccag agccgggccc gcaccaagga cagcatcgcc
      661 tgggtcaatt gcagatcgcc ccgcaattga tgtgcttgag tgttttcttc gattcgcttg
      721 gtcaggtcct cgagctggtc ggccagggcc gtgagctggc ttcccagctg atcttgcccc
      781 cgtccctggt caccaagttg cgcctgcaga tcctcgagtt gaacttgtaa ttcggccacc
      841 gactcctgct gctcggcgag cctgctcact gcctccggca gctgagcttc cagggcggcg
      901 atccgggccg aattctgccg agtgggctcg cccaattggc gaatggcgag cgtcactccg
      961 aaatacgctc ctgccccaag cgcaactccc aggagcacca ccagggcaag ccgcagcaag
     1021 accttgctga cccgcgccag gagcgcgggt tgaggccgct cgtcgcttcc tgcggtcggt
     1081 tcagcctgac cagtggtcat ccgggaccca aacctgcggc gtcttagggc gcgccacgaa
     1141 ggccctcacc agcaacagtc ccaccacaaa tccaccgaca tgcgcccacc aggcgattcc
     1201 gccaaaatcg ccgatcgccc caagcgatag cagtccttga gagagttggg tgaaaaacca
     1261 gatgccgatg aacacgatag ccgggacgtc gacaaaccag ggcaagaaga aaagcgggat
     1321 gaaagtggtc acccgagccc ggggaaagag gatgaaatag gcgcccagca cgcccgcaat
     1381 ggcaccgctg gctcccacgg tcggaatggt tgagtttggg ctggagactg catgggccag
     1441 cccggcaacc gtgccggcaa tcagatagaa gcccagatag cgacccgatc ccatctgatc
     1501 ctcgacgttg tcgccgaaga tgaaaagagt ccacatattg ctgagcaagt gaaaccaacc
     1561 accatgcaaa aacatcgagc tgaacagggt cagcccggcc agcggctgtt caaaggagag
     1621 ccgcgccggc actaccccaa actcacccaa gaaccgctcg tatccactcg gcccgagcga
     1681 ggtcacgaag agaaagctga gcacattggc cgcgatcaga gcatagttga cgaccggaaa
     1741 ccgcctcgat cgaaccgcgt ctcgaattgg aaacagggaa ggatcctccg gctgtggggg
     1801 aaaacaaatg ggcggcgagc gccgcccatt gtaccccatg cgattcgggg ggagttactt
     1861 ggccttgact tcgatcttct tcgggcgggc ctcttcagcc ttgggcaccc gcacggtcag
     1921 cagaccgttc tcgatcttcg cctcaactgc ctcggcatcg atgacctccg gaagctgaat
     1981 gcgccggtag aacccaccgg accggtcgag ctcgctgatc agagcttcgc ctgcgctggc
//
LOCUS       DATDPQ010000137         8076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_315830, whole genome shotgun sequence.
ACCESSION   DATDPQ010000137 DATDPQ010000000
VERSION     DATDPQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315830"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VI520_03290"
     CDS             complement(<1..265)
                     /locus_tag="VI520_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36940.1"
                     /translation="MGSGRSGGLILIVLGIAVAGIGTLYVLSQLSSGELQTGGAVVGV
                     GIFGLPALLAIGFGVALLSRSREVAAGDVQQAQLRKILDMVKSK"
     gene            complement(268..2139)
                     /locus_tag="VI520_03295"
     CDS             complement(268..2139)
                     /locus_tag="VI520_03295"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HLF36941.1"
                     /translation="MRWVLLLALGLLLVGWPRSAAAQGKTLYWERFDVTLAIQPNGDL
                     KVVERQRIRFTAGSFTYGFALIPLDKTQGIDQVSLSEPGGTVYQEASFGEEPFTFETS
                     QVENELEVRWYFPPTADSARTFDLAYTVHGAIRVYDSGDKLQWFAIDDERDFPIEESS
                     VTVLLPPGAAFQIIDSAGAEATWSQSADGTSVTFLPTGPLSSTDVVEVGIEFSHGAIP
                     AQVPGWQAADDDVNYYNDNVKDLVDLGLGVLAVLILLGGPALIYFVYYARGRDPKVGP
                     VPEYLAEPPGDLPPGIMGTLLDEQAEMRDVVATIVDLARKGSLRIEETANKGLLGLGS
                     TDFIFHRQASGADGLNPVESEVVAGLFRGGKNSVEMSDLREKFYTRLPAIQSKLYQEM
                     VSRGFFRTNPDTTRKLWRGLGGAVLVGAFFLGMFATGALSSYTSLLPCVFGALGLTGL
                     IALVSGGAMPAKTRKGAEAAAQWKAFKTYLERIDKLADLQQVGELFERYLPYAIALGI
                     KDSWIHKFSQQPMTPVPGWYMPYYGGGGSRPTVSTGGKGGGTAVPPLRGPGGLQGMSE
                     GLAGGLQSMSDGLTRMLNSAGRVLQSAPSSSGRSGGGFSGGGFSGGGGGGGGGRGFG"
     gene            complement(2257..2781)
                     /locus_tag="VI520_03300"
     CDS             complement(2257..2781)
                     /locus_tag="VI520_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36942.1"
                     /translation="MSQTVRRRLGPGGGVPTPAEHDLVLVAILNRPRDLEIARTLGWY
                     RIPLGTAPRTVRVNWLAFYLPAAFDGQRWSVRYLAEVRGIELLTRRELLYLEADHPRA
                     AHPYYKLQLGPLMELEPPIPSRSWRRFSFLYTTGDRLYRAADLTELRVPASRAREQLW
                     RMLRDRAGFVQAAA"
     gene            complement(2872..3246)
                     /locus_tag="VI520_03305"
     CDS             complement(2872..3246)
                     /locus_tag="VI520_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36943.1"
                     /translation="MKRTLVTVGVFVILMASLTACAGGDINDAAQAFMTAVANGDYAT
                     AFDLSGPQLQAEVGGVEGLQAALPGALTDWKFDSISQDNNTATMAGTATGPDGLSYTV
                     SLYFEKLGDAWKVEGYAADLVP"
     gene            complement(3371..4840)
                     /locus_tag="VI520_03310"
     CDS             complement(3371..4840)
                     /locus_tag="VI520_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLF36944.1"
                     /translation="MIDLTFHPDRLDRAAQRARERSIVIPTFEQMRDPSRIPAAIRER
                     LSTVGLWDLDPLNLFRINWKNEPIGSGGGFGRVNVLEFPPSLTGVEARILALVGKWFP
                     TGAHKVGAAFACLVPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLGCQSIAILPE
                     GMSKERFEWLATIAGEVIKTPGSESNVKEIFDKTWELRRSGEDLMIFNQFEEFGNYLW
                     HYDVTGHAMLELLERELGPQENFRGVALTTGSAGTLACGDLLKQHFPASKIAAGEAAQ
                     CPTLLENGFGAHRIEGIGDKHVPWIHNVKNTDLVIDLDDAGIVALARLFNEPSGRQYL
                     TARGVPEPTVEKLDLLGFSGIANLTMAIKFAKYYELGSADVVLTVLTDSMDLYRSRLD
                     EMRADYGEYREPQAAADCARYLHGLTTEGLLELRYTDRRRIHNLKYFTWVEQQGRTHD
                     ELVAQWVDPAYWSGVQGQVSAIDERIQAFNERVGLLEAV"
     gene            complement(4837..5799)
                     /locus_tag="VI520_03315"
     CDS             complement(4837..5799)
                     /locus_tag="VI520_03315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF36945.1"
                     /translation="MKATVTEPTFSIVGPVFDEVGTIRAFYARLRNAMDQLKEPWELL
                     LIDDGSRDGSTDLIRDLGREDPRVRPVLFSRNFGHQIALTAGLDYSRGQAVVVMDTDL
                     QDPPELIAELVGKWREGFEVVFAVRTEREGESWFKKATAALFYRLINRITDVDIPLDT
                     GDFRLLDRKVVEVVKQMRERHRFPRAMVAWVGFRQTGVPYKRAARSSGETKYPLRKMM
                     RLAINAVTGFSYFPLQLATYFGFICAGLSAVAIPLVVLIRLSGRSALLGQATTLIAVL
                     FLGGVQLISLGILGEYIGRLYDEAKGRPLYVLREEPGPIEERER"
     gene            complement(5850..7097)
                     /gene="thrC"
                     /locus_tag="VI520_03320"
     CDS             complement(5850..7097)
                     /gene="thrC"
                     /locus_tag="VI520_03320"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLF36946.1"
                     /translation="MKFTGYRCSICGEQYGPDRMIYICLKDGGNLDVLLDLGSIRKRG
                     SPEQISASSDGSMWRYLPLLPVDDPGYPNSALRAVGWTPLLKAERLGTDLGVAELWLK
                     DDGRNPTASFKDRASALVVVRAREVGAETVITASTGNAGAALAGMAAAAGLPAVILAP
                     RTAPPAKVAQLLIFGARVILVEGSYDEAFDLTLEAAQAFGWYCRNTGYNPFTAEGKKT
                     AAFEICEQLTPGRSKGLWAAPDAVFVSVGDGNILTGLHKGFTDLFELGWIERIPRLYG
                     VQSSGSAAVANAFRAGSSSIQPVKATTLADSIAVDLPRDGIRALRAARETGGAVFTVE
                     DSAILEAIRTLGQAAVFAEPAAAASYAGVVQGVEEGLIGSADQVVAVITGSGLKDVSA
                     ALRAAGSPLVIEPTLKALKAALG"
     gene            complement(7132..>8076)
                     /locus_tag="VI520_03325"
     CDS             complement(7132..>8076)
                     /locus_tag="VI520_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF36947.1"
                     /translation="AGVATGTVLLFATIGEIFAERAGVLNLGLEGMMLIGAMSAFSVT
                     VATGNGLLGLIVAMLAAGGLSLLHGVVTIHFQADQVVSGLALTFLGTGLSLVFGEGLN
                     KAGAISLLPTLTIPVLASIPVLGPILFTKQSVMVYLGYLLVPVAWFYINRTRPGLHLR
                     AVGENPTAADAMGLNVYRLRYSYVMVGGSLAGLAGGTISLAISPGWFSELTTGGMGWI
                     AVGLVIFAQWDPWRAAFGGYAFGALRRTILDIQGPQTLLGLRNPFFFNPYFGFFLQML
                     PYAFTIAVLIIGSREALRKRLGAPAALGLPYVRGERGH"
BASE COUNT         1533 a         2684 c         2433 g         1426 t
ORIGIN      
        1 ccttgctctt caccatgtcc aggatcttgc gcagctgggc ttgttgcacg tcgcccgccg
       61 ctacctcccg cgagcgcgag agcagcgcga ccccgaagcc gatcgccagg agagctggca
      121 aaccgaagat tccgacaccg accaccgccc cgcccgtctg gagttcgccg gaactgagct
      181 ggcttagtac gtacagggtc ccaatgccag ctacggcgat tccaagcaca atcaggatca
      241 gccctccgct tctaccgcta cccatcgcta accgaagcct cgcccaccgc caccgccgcc
      301 tccgcccccg ctgaagcctc caccgctgaa tccgcccccg ctgcgaccgc tcgaggaggg
      361 ggcagactgg agcactcggc cggcgctgtt cagcatccgg gtcaacccgt cgctcatgct
      421 ctgcaagccg ccagccaggc cctccgacat tccttgcagg cctcccggac cgcgcagcgg
      481 cggaaccgcg gtgcctcccc cttttcctcc ggtcgagacg gtgggtcgcg accctccccc
      541 tccgtagtaa ggcatgtacc agccggggac cggagtcatg ggttgctgtg aaaacttgtg
      601 aatccaggaa tctttgatcc ccaaagcaat cgcatagggc aggtagcgct cgaagagctc
      661 cccgacctgt tgaagatccg ccagcttgtc gatccgctcc aaataggtct tgaaggcttt
      721 ccactgagca gcggcctcgg cgcctttgcg agtcttggcc ggcatcgccc cgccggagac
      781 caacgcaatt agcccggtga ggccgagggc cccgaacaca caggggagca ggctcgtgta
      841 gctcgaaagc gcgcccgtgg caaacatccc gaggaagaac gccccgacca ggaccgcacc
      901 ccccaggccg cgccagagct tgcgagtcgt atccggattg gtgcggaaga accctcggct
      961 gaccatctct tgatagagtt tgctttgaat ggccggcaac cgggtgtaga acttctcgcg
     1021 cagatcgctc atctcgaccg agttcttccc gccccggaag agcccagcaa cgacctcgct
     1081 ctcgaccggg ttgaggccat ccgcgccgga ggcctgccgg tgaaagatga aatcggtgga
     1141 gccaaggcct agcaaacctt tgttggcggt ctcttcgatt cgcaagctgc ctttgcgggc
     1201 caggtccacg atcgtggcga ccacgtcccg catttcggcc tgctcgtcca gcaaggtgcc
     1261 catgattcca ggtggcagat cgccaggcgg ttccgccagg tattccggca ccgggccgac
     1321 cttaggatcc cggccgcggg cgtaatacac aaaatagatc agggccggcc cacccagcag
     1381 gatcaacacg gccaggacgc caagccccag gtcgacgagg tccttgacgt tgtcgttgta
     1441 gtaattcaca tcatcgtcag ccgcctgcca gccgggcacc tgggccggga tagcgccgtg
     1501 gctgaactcg atgccgactt cgacaacgtc cgtgctcgat agtggaccgg tgggcaggaa
     1561 ggtgacgctc gtgccgtcgg cactttggct ccaggtcgcc tcggcgccgg ccgagtcgat
     1621 gatttggaac gcggcccccg gaggcaataa tacggtgacg ctgctctctt caattggaaa
     1681 gtcacgctcg tcatcgatcg caaaccactg cagcttgtcg ccgctgtcgt agacccggat
     1741 cgcgccatgc accgtgtaag cgaggtcgaa ggtgcgagcc gagtcggcgg ttggagggaa
     1801 ataccaacgg acttccagtt cgttctcaac ctgggaggtc tcaaaagtga acggctcctc
     1861 accgaaggat gcctcctggt acacggtccc tcccggctcg ctgaggctca cctggtcgat
     1921 gccttgggtc ttgtccagcg ggatgagcgc gaaaccgtag gtgaaggatc ccgcggtgaa
     1981 gcggatccgc tgccgctcga ctaccttaag gtcgccgttc ggctgaatcg cgagggtgac
     2041 gtcgaaccgt tcccaataga gggtcttgcc ttgggcggcc gcggacctcg gccagccgac
     2101 aaggagcagc ccgagcgcaa gtagcaggac ccatcgcaat gatgctcgga ccgaatgatt
     2161 cagcggggga aacaccgggg tgatgatacc tgcaacccgt ggcgaagtcg agccgccgcg
     2221 cttcctattc ggcggacaca ggccagagca gagaagtcag gccgcagctt ggacgaagcc
     2281 agctcgatcg cggagcatcc tccacagctg ctcacgggcg cgggaagcgg gaaccctgag
     2341 ctcggtgaga tcggccgcgc ggtacagtcg atcgccggtg gtgtacaaga agctgaatcg
     2401 ccgccaactg cgggagggga tcggcggttc gagttccatc agcggcccca gctgcaactt
     2461 gtaataggga tgagcggccc gcgggtggtc ggcctccagg tacagcagct ctcggcgggt
     2521 cagcagctcg atcccccgca cttcggcgag atagcgtacc gaccagcgct ggccatcgaa
     2581 tgcggccggc aggtagaagg ccagccagtt cactcgaacc gttctaggcg cagtcccgag
     2641 cgggatccga taccagccta aagtccgggc gatctcaagg tcccggggcc ggttcaggat
     2701 ggcgaccagc actaggtcgt gttcggccgg ggtgggaacc ccgccgcctg gcccgagccg
     2761 gcggcgcacc gtttgcgaca tgccgaggca ctcctactac tcactacagt agatcatttg
     2821 ttctaggttg tcaagtggcc tggggaggca tcctggccgt accagtgccg gtcagggtac
     2881 cagatctgcg gcgtacccct ccaccttcca ggcgtcccct agcttttcga aatagagaga
     2941 taccgtgtac gacagcccgt cgggacctgt tgccgtccct gccatggtgg ccgtgttgtt
     3001 gtcctggctg atggagtcga atttccaatc ggtcagggcc cccggaagtg ccgcctggag
     3061 accttcgacg ccgccgactt cggcctgcaa ttgtggaccg gaaaggtcaa aggcagtcgc
     3121 atagtccccg ttggccaccg cggtcatgaa cgcttgggcc gcatcgttga tgtccccacc
     3181 tgcgcaggct gtgaggctcg ccatcaggat gacaaataca ccgacggtca ccagggttct
     3241 cttcatgtga ggctcctttt gctccaaagt gggcgtagtt tgggacaaat gcacatcgcg
     3301 cgccatagga aatgcgggcg gcaatgcaac cattgtggat cgaacttgtc ttgtttgccg
     3361 tcgccttcga tcagacggct tcgagcaatc ccacccgctc gttgaacgcc tggatccgct
     3421 catcgatcgc ggacacttgt ccctgcaccc cgctccagta ggccggatcg acccactggg
     3481 caacgagctc atcatgggtc cgcccttgtt gttccaccca ggtgaaatac ttcaagttgt
     3541 ggatccgccg ccggtcagtg tagcgcagct ccagcaaccc ctcggtggtc agcccgtgca
     3601 gatagcgggc gcaatcggcc gcggcctggg gctcgcggta ctcaccgtag tcggcgcgca
     3661 tctcgtccag ccggctccga tacagatcca tcgagtcggt caggacggta agcaccacgt
     3721 ccgccgagcc gagctcgtaa tatttggcga acttgatggc catcgtcaaa ttggcgatcc
     3781 ccgagaaacc aagcagatcc agcttttcga ccgtgggttc ggggacaccc cgggcggtca
     3841 ggtactgtct gcccgagggt tcgttgaaaa gtcgggccag cgcgacgatc ccggcgtcgt
     3901 cgaggtcgat caccaggtcg gtgttcttga cgttgtgaat ccagggcacg tgtttgtcgc
     3961 cgatcccctc gattcggtgg gccccgaagc cgttctccag tagggtcggg cactgggcag
     4021 cctcgccggc cgcgatcttg cttgccggga agtgctgttt gagcagatcg ccgcaggcga
     4081 gggtccccgc cgagccggtg gtcaaagcga cccctcggaa attctcctgg ggcccaagtt
     4141 ctcgctcgag cagctccaac atcgcgtgcc cggttacgtc gtagtgccac agataattcc
     4201 cgaactcctc gaattggttg aagatcatca gatcttcgcc cgagcggcgc agctcccagg
     4261 tcttgtcgaa gatctctttg acattcgact cgcttccagg agtcttgatc acttcaccgg
     4321 cgatcgtggc cagccactcg aagcgttctt tgctcattcc ctccggcagg atggcgatcg
     4381 actggcagcc gagcagggcg gaatcgtaag ctccaccgcg gcagtaattc ccggtcgagg
     4441 gccagacagc cttctgggtc gtggggtcga actggccggt caccaggcgc ggcaccaggc
     4501 aggcgaaggc cgccccgacc ttatgggcgc cggtggggaa ccacttccca accagcgcca
     4561 atatgcgagc ctcgaccccg gtcaggctcg gcgggaattc caagacgttg accctcccga
     4621 accctccgcc cgagccgatc ggctcattct tccagttaat gcggaacagg ttgagcggat
     4681 cgaggtccca caggcccacg gtactcagcc gttcccggat cgcggccggg atccggctcg
     4741 ggtctcgcat ctgctcgaag gtcgggataa cgatgcttcg ctctctggcc cgctgcgcag
     4801 cgcggtccag ccggtccggg tggaaggtga ggtcgatcat cgttcccgct cctcgatggg
     4861 tcccggctcc tctcgcagga catatagcgg ccggcccttg gcttcgtcgt acaaccggcc
     4921 aatgtactcg ccgagaatcc ccagcgagat caactggacc ccgccaagga acaataccgc
     4981 gatcaaggtg gtcgcctgcc cgagcaatgc tgatcgcccg ctcaaccgga tcagcacgac
     5041 gagtggtatc gcgaccgcgc tgaggccggc gcagatgaat ccgaaatagg tcgccagctg
     5101 gagcgggaag taggaaaacc cggtaaccgc gttgatggcc agccgcatca tcttgcgcag
     5161 cgggtactta gtctcaccgg aggaacgggc ggcccgcttg tacggcaccc cggtctgacg
     5221 gaaaccgacc caagccacca tggcccgcgg aaagcggtgg cgctcccgca tctgcttcac
     5281 cacctcgacc accttccgat ccaacagtcg gaagtcgccg gtatctagcg ggatatccac
     5341 gtcggttatc cggttgatca gccgatagaa aagagccgca gtcgccttct tgaaccagga
     5401 ctcgccttct cgctcggtgc ggaccgcgaa gacgacctcg aaaccctccc gccatttgcc
     5461 gaccagctcc gcaatcagtt cgggtggatc ttgcaggtcg gtatccatga ccactacggc
     5521 ctgcccccgg ctgtaatcca gcccggccgt cagtgcgatc tgatggccaa agttgcggga
     5581 gaacagcacc ggccggaccc gaggatcctc gcgccccagg tcgcggatga ggtcggtcga
     5641 gccgtcgcgg ctgccatcgt cgatcagcag cagctcccag ggctccttga gctggtccat
     5701 ggcgttccgc agccgggcat agaaggcgcg gatcgtcccg acctcatcga agaccgggcc
     5761 gacgatcgaa aaggtgggct ccgtgacagt ggctttcaat ttgcgatctc gtagcatacg
     5821 cgaactcaca tccatccgct agccgtgtct catccgaggg cagccttcag tgccttcaaa
     5881 gtcggctcga tgaccagcgg gctgccggcg gcccgcagtg cagccgatac gtccttcagc
     5941 ccgctcccgg tgattaccgc caccacctgg tccgctgaac cgatcagccc ctcttccacg
     6001 ccctgtacca cgccggcata agaggccgcc gcggccggct ccgcgaatac cgcggcttgg
     6061 ccgagggtgc ggatggcttc caggatcgca ctgtcttcga ctgtgaatac cgccccgccg
     6121 gtctcccggg ccgcccgcag cgcccggatc ccgtcgcggg gaagatcgac cgcaatgctg
     6181 tcggccagcg tggtagcttt caccggttgg atgctcgagc tgcccgcccg aaaggcgttc
     6241 gccaccgcgg ccgacccgct cgactgcaca ccgtagagcc gggggatacg ctcgatccag
     6301 cccagctcga acaggtctgt gaaacccttg tgcaacccgg tcaggatgtt cccgtcgccg
     6361 accgatacga acaccgcatc cggggcggcc cacaagccct tcgaccgccc cggcgtcagc
     6421 tgctcgcaga tctcgaatgc ggccgtcttt ttcccctctg cggtaaacgg gttgtatccg
     6481 gtgttccgac aataccagcc gaaagcctgg gctgcctcca gcgtcaaatc aaaggcctca
     6541 tcatagctgc cctcgaccag gatcacccgg gcgccaaata tcaggagctg ggcaaccttc
     6601 gccggcgggg ccgttcgggg cgccaggatc actgcgggca ggccggcggc cgccgccatc
     6661 ccggcgaggg ccgctccggc gttgccggtc gaagcggtta tgacggtctc ggccccgact
     6721 tctctggccc ggacgaccac cagcgcgctg gcccggtctt taaaggaagc ggttgggttg
     6781 cgcccatcgt ctttcaacca gagctcagcg actcccaggt cggttcccag acgttcggcc
     6841 tttaacaacg gcgtccagcc gaccgcccgc agtgcgctat ttggataacc tggatcatcg
     6901 accggaagca ggggtagata gcgccacatg gacccgtctg agctggcact gatctgctcg
     6961 ggcgagcccc gtttgcggat actcccaaga tcgagcagca catcgagatt gcccccgtcc
     7021 ttcaggcaga tgtagatcat tcgatccgga ccgtactgtt ctccgcagat cgagcaccga
     7081 tatcctgtga atttcatggt ggtttgaggg gcttgcagta ttcagccggc tctagtggcc
     7141 gcgttcgccg cggacatatg gcaacccgag agcggccggc gcgcccaatc gcttgcgcag
     7201 cgcctcccgc gaaccaatga tcaacaccgc gatcgtgaag gcatacggca gcatctgcag
     7261 aaagaacccg aaataggggt tgaaaaagaa cggattccga agaccgagca gggtctgcgg
     7321 accctggatg tcgaggatcg ttcgacgcaa cgccccgaat gcataccccc cgaaggcagc
     7381 ccgccacgga tcccactggg caaagatcac cagcccgacc gcgatccagc ccattccgcc
     7441 ggtcgtcagt tcgctgaacc agcccggtga aatcgccagg ctgatcgtgc cacccgccag
     7501 gccggccagc gaccctccga ccatcacata ggagtagcgc aagcggtaca cattgagacc
     7561 cattgcatcc gccgcagtgg gattctcgcc aaccgcccgc agatgcagtc cggggcgggt
     7621 gcggttgata tagaaccagg cgacgggcac cagcaggtac cccagataga ccatgacact
     7681 ttgcttcgtg aacagaatgg ggccgaggac cggaatgctg gccaggactg ggatggttag
     7741 ggttggcagg agcgagatcg cgccagcctt gttcagtcct tcgccgaaaa ccagactcag
     7801 cccggtaccc aggaacgtga gcgccagacc gctcacgacc tgatctgcct gaaaatggat
     7861 ggtgaccacc ccatgcagca gcgacaaccc tccggcggca agcatcgcca cgatgaggcc
     7921 gagcaatccg ttcccggttg ccacggtgac gctgaatgcg ctcatcgcgc caatcagcat
     7981 catcccctcc agacccagat tcaatactcc ggcccgctcg gcaaagatct ccccgatggt
     8041 tgcgaagagt aggaccgttc cggtcgccac ccccgc
//
LOCUS       DATDPQ010000138         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_836388, whole genome shotgun sequence.
ACCESSION   DATDPQ010000138 DATDPQ010000000
VERSION     DATDPQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836388"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VI520_03330"
     CDS             complement(<1..633)
                     /locus_tag="VI520_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF36948.1"
                     /translation="MTATRILLVDDHEVVRLGLQSLLDARKDMRVVAAVGTAEEALEA
                     AHAHHPDVVLMDIRLPGRSGIEATERISRELPDTKVIILTSYAEDEMLFAAIRAGAVG
                     YVLKQVGTDDLVRAIEAAARGEASLDPALTQRVFSEIRKSIRQQDASAFSELTEQELR
                     VLALVTEGRTNRQIAQALFLGEGTVRNYVSNILSKLGVSNRAEAAAYAVKH"
     gene            835..1308
                     /locus_tag="VI520_03335"
     CDS             835..1308
                     /locus_tag="VI520_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HLF36949.1"
                     /translation="MKFLIGGLLIVAAVVYLIVSSTQAAAQYYLTIDEMRSMGSQVYG
                     RPLKLAGAVDGDSIAYDSATLTLSFSMANVPAELDVIEAGGGLAKVLHDSLLDPAASR
                     LQVIYVGPKPDLIRHEAQAIVTGELGQDGVFRADELLLKCPTRYEEEVPLQVVEG"
     gene            1389..>2593
                     /gene="ccsA"
                     /locus_tag="VI520_03340"
     CDS             1389..>2593
                     /gene="ccsA"
                     /locus_tag="VI520_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLF36950.1"
                     /translation="MTEEIGYTALVMAFLLALYGAAAAAFGAKRQRPELVESARRAMV
                     LSWPLISVAALAIIALLVSGRYDVEYVAKVTSSTMPPYLKVTALWGGQAGSLVFWSWL
                     MAAFASAVGLRRWDRDRDLLPWVIVVTMVTLAFFLSLVIFVENPFRRLWLDPVGNTVT
                     AFLRPAGARPLVPADGQGLNPLLRHPGMIIHPPMLYLGFVSFVIPYAFAIAALVTRRT
                     DDRWIRVTRRWTLVAWLFLSLVIFVENPFRRLWLDPVGNTVTAFLRPAGARPLVPADG
                     QGLNPLLRHPGMIIHPPMLYLGFVSFVIPFAFAIAALVTRRTDDRWIRVTRRWTLVAW
                     LFLALGLILGGRWAYDVLGWGGYWGWDPVEIAAFMPFLSGTAFLHSVMVQEKKGMLKH
                     WNVLLIILT"
BASE COUNT          408 a          813 c          828 g          544 t
ORIGIN      
        1 gtgcttcacc gcataagccg cggcctcggc ccggttggag acgccgagct tggagaggat
       61 gttgctcacg tagttgcgga cggtgccctc gccgaggaac agcgcttggg cgatctggcg
      121 gttggttctg ccctccgtga ctaaggccag cactcgcaat tcctgttcgg tcagctcgga
      181 aaaggccgag gcatcttgct gtcggatgga cttgcggatc tcggagaaga cccgctgggt
      241 caaggccgga tcgagcgagg cttcgcctcg ggcggccgct tcgatggccc gaaccaggtc
      301 atcggtaccg acctgcttga gcacatatcc gaccgccccg gcccggatcg ccgcgaacag
      361 catttcgtcc tcggcgtagg aggtcaggat gatcaccttg gtatcaggga gctcccggct
      421 gattcgctcg gtggcctcga tcccgctgcg gcccggcagc cggatatcca tcagcacaac
      481 gtcgggatga tgggcgtggg cggcctccag cgcttcctcg gccgttccga ccgccgccac
      541 gaccctcata tccttgcggg catccagcag gctctgcagg ccaagccgga cgacttcgtg
      601 atcgtcgacc aggaggatgc gggtagctgt catagccaga agtataccca gccacggttc
      661 tcattccacg attctggggg cttcatctac aattaatctg ggcgccggaa gatggtggta
      721 agaacaaaat acgccgtctc ggtgactttt gtctccgcct cccgatgata caatctcgct
      781 cggtcgacaa tcttggacac cccagtgagt tctcaggttg aatccggcgg ccgagtgaag
      841 tttctgatcg gcggcctgct gatcgtggcg gcggtggtct acctcatcgt ctcgtctacc
      901 caggccgcgg cccagtacta tctgacaatc gatgaaatgc gcagcatggg cagccaggta
      961 tacggccgcc cgctcaaact tgccggggcg gtggatgggg actcgatcgc ctacgactca
     1021 gcgaccctga ccctgagctt cagcatggcc aatgtgcccg ctgagctcga cgtcatcgaa
     1081 gcgggcggcg ggctggccaa ggtgctgcat gattcgcttc tcgatccggc ggcctcccgg
     1141 ctgcaggtga tctacgtcgg tcccaagcca gatctcatcc ggcacgaagc gcaggccatc
     1201 gtgaccggcg aactgggaca ggatggggtc tttcgggccg atgagctatt gctgaaatgc
     1261 ccgacccgct atgaggagga ggtacccctc caagtcgtcg aaggctagcg ggggcgatcg
     1321 aagatcatga ggtcgaacgc cgacggcagc acggaagctg gccgtcggcg ttgcgcgagg
     1381 gctaagaaat gactgaggaa atcggataca cggcattggt gatggcattc ctgttggcgc
     1441 tctatggggc cgccgcggcg gccttcggtg ccaagcgcca acgtcccgaa ctggttgaga
     1501 gtgcccggcg ggcgatggtg ctctcctggc cgttgatcag cgtggccgcc ctggcgatca
     1561 tcgccctgtt ggtctccgga cgctatgatg tcgagtacgt cgctaaggta accagcagca
     1621 cgatgccgcc ctatctgaaa gtcactgcgc tgtggggcgg gcaggcgggc tcgctggtct
     1681 tctggtcctg gttgatggcg gccttcgcca gtgcagtcgg gctgcggcgc tgggatcgcg
     1741 atcgggatct gctgccctgg gtgatcgtgg taacgatggt caccctggcc ttcttcctct
     1801 cgctggtgat cttcgttgag aatcccttcc gccggctttg gctcgacccg gtgggtaaca
     1861 cagtgaccgc cttcctccgt ccagccggcg cccgtccgct ggtgcctgcc gatgggcagg
     1921 gcctcaaccc gctgctccgt cacccgggca tgatcatcca tcccccgatg ctgtatctgg
     1981 gcttcgtgtc cttcgtaatc ccgtacgcct ttgccatcgc agcgctggtg acccgccgca
     2041 cggacgatcg ctggatccgg gtcacccggc gctggactct ggtcgcttgg ctgttcctct
     2101 cgctggtgat ctttgtcgag aatcccttcc gtcggctttg gctcgacccg gtgggcaaca
     2161 cggtgaccgc cttcctccgt ccagccggcg ctcgtccgct ggtgcctgcc gatggacagg
     2221 gcctcaaccc gttgctccgt cacccgggca tgatcatcca tcccccgatg ctttatctgg
     2281 gcttcgtgtc cttcgtaatc ccgttcgcct ttgccatcgc ggctctggtg acccgccgga
     2341 cggacgatcg ctggatccgg gtcacccgtc gctggacctt ggtcgcctgg ctgttcctgg
     2401 cactggggtt gatcctgggc ggccgctggg cctacgacgt gctgggctgg ggaggttact
     2461 ggggttggga cccggtcgaa attgcggcct tcatgccctt tctaagcgga acggccttcc
     2521 tccactcggt gatggtgcag gagaagaagg ggatgctcaa gcactggaac gtgctgctga
     2581 tcattctgac cta
//
LOCUS       DATDPQ010000139         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_836514, whole genome shotgun sequence.
ACCESSION   DATDPQ010000139 DATDPQ010000000
VERSION     DATDPQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836514"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VI520_03345"
     CDS             <1..1294
                     /locus_tag="VI520_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HLF36951.1"
                     /translation="LEVGEGVVEVKATNGDTHLGGDDWDQRILNWIADEFKKDQGIDL
                     RQDRQALQRLREAAEKAKIELSTVSESEINLPYVSADASGPKHLQLKLSRAKFEQLSE
                     ELVARIHGPFDAVLKDAGLKPSEVDEVVLVGGATRMPMVQDLVRSRTGKEPHKGVNPD
                     EVVAVGAAIQAGVLAGDVKDVLLLDVTPLSLGVETLGGVMTTLIERNTTIPIKKAETF
                     STAEDSQTAVDIHVLQGERSMAGDNMTLGRFRLEGLPPAPRGIPQVEVTFDIDANGIL
                     NVNAKDKATGKQQSVTITASTNLSKQDVERMVEQGKQHAAEDRTHKELADARNNGDNL
                     AYQAEKALRELGDKVPQADRERIEGKVKELREAVQGDDLSQIKKLSEEVQQASYALSQ
                     QLYAQQGQTPQPQPEGGPQGSTEGEGDDDVVEGEFREA"
     gene            1392..1820
                     /locus_tag="VI520_03350"
     CDS             1392..1820
                     /locus_tag="VI520_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HLF36952.1"
                     /translation="MTKAALQDLQLEVEEALWQYDPVREALGVLQIEARPDGQVVIAG
                     PVRTGSIKDGILATVRRLPGVVGVIDRMLPDHELELAIAKAIDADLSLKALPPGSVSV
                     YSHLGQVSLVGKTPTEALRRRAIEVAGGVPGVRRLVDRLR"
BASE COUNT          428 a          576 c          696 g          335 t
ORIGIN      
        1 gctcgaagta ggtgaggggg tagtcgaagt caaagctacc aatggcgata cgcacctggg
       61 gggcgacgac tgggatcagc ggatcctgaa ctggatcgcc gatgagttca agaaagatca
      121 aggcatcgac ctgcgccaag atcggcaggc gctccagcgc ctgcgggagg cggctgaaaa
      181 ggccaagatc gagctatcca ccgtgagtga gtccgagatc aacctgccct atgtctcggc
      241 ggacgcctcc ggcccgaagc acctgcagct gaagctgagc cgagccaagt tcgagcaact
      301 ttccgaagaa ttggtggccc gaattcacgg gccgtttgat gcagtgctca aggatgccgg
      361 gttgaagccc agcgaggtgg acgaagtggt gctggtgggt ggggctaccc ggatgccgat
      421 ggtgcaagac ctcgtgcgct cccgcaccgg caaggagcca cacaagggtg tcaacccgga
      481 tgaagtggtg gccgtggggg ccgccatcca ggccggggtg ctggccggcg atgtcaagga
      541 tgtcttgctg ctggacgtca ccccgctttc gctcggggtc gagaccctgg gtggcgtgat
      601 gaccaccttg atcgagcgca atacaaccat cccgatcaag aaggccgaga ccttttcgac
      661 tgccgaagac agccagaccg cggtagacat ccatgtgttg caaggcgaac gctcgatggc
      721 gggcgataac atgaccctgg gacgtttccg gttggaaggt ctgccgccgg cgccgcgggg
      781 aattccgcag gtcgaggtga ccttcgatat cgacgccaac gggatcctga acgtgaacgc
      841 taaagacaag gctaccggca agcagcagtc ggtcacaatc accgcctcca ctaacctgag
      901 caagcaggac gtggagcgga tggtcgagca gggaaagcag cacgccgctg aggaccgaac
      961 tcacaaggaa ctggccgacg cccgcaacaa cggcgacaac ctggcctacc aggccgagaa
     1021 ggccctgcgc gaacttggcg ataaagtccc tcaggccgac cgcgagcgga tcgagggaaa
     1081 ggtcaaggag ctgcgggaag cggtccaggg ggacgacctc agccagatca agaagctgtc
     1141 cgaagaagtc cagcaggcga gctacgctct gagccagcag ctgtatgcgc agcagggcca
     1201 gacccctcag ccccagccgg agggagggcc gcaaggctca accgagggcg aaggcgacga
     1261 cgatgtcgtg gagggcgagt tccgggaagc ataagtctcg ggacctgttg aatgtcacgg
     1321 gcgggtcagg aaatctgacc cgcccgtttt gattgggtcg aaggctataa tttcgggcgg
     1381 cggaggtggg gatgaccaag gccgcgctgc aggatctgca gcttgaggtc gaggaggcgc
     1441 tctggcagta cgatccggtg cgcgaagcgc ttggcgtgct gcagatcgag gcccggccag
     1501 acggccaggt ggtgattgcc ggcccggttc gaactgggtc gatcaaggat ggcatcctcg
     1561 ccaccgttcg ccggctgccg ggggtggtcg gggtaataga tcggatgttg cctgaccatg
     1621 agctagagct ggcgatcgcc aaggccatcg acgcggatct aagcctcaaa gcgctgcctc
     1681 cgggcagcgt atccgtttac agccatctgg ggcaagtttc tctggtaggc aaaactccca
     1741 ctgaggccct gcggcgaagg gccatcgagg tagccggagg agtgccaggt gtccggcggc
     1801 tggtcgaccg gttgcgctag cccacagatg accgttttcc catgggctta ggagttcttt
     1861 ccttgtcagg tcttaaaggc acaagatata atgggttgcc agacccttcg cccgcgccga
     1921 attggcgggc tgagttccct ccggaagtgg tcgggctacc tgaacttcgc tcggccgctg
     1981 ccgctccgac ccgcggagca agagacattc tggcaggagg tgttgccacg tgatc
//
LOCUS       DATDPQ010000140         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_836638, whole genome shotgun sequence.
ACCESSION   DATDPQ010000140 DATDPQ010000000
VERSION     DATDPQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836638"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VI520_03355"
     CDS             complement(<1..351)
                     /locus_tag="VI520_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36953.1"
                     /translation="MERLSLTLDIRPNEIGSKRINVRSSLLVANLLAEIQDKFNIDGT
                     LQLMLGQDEGPLSLRSPLEHAGVIEGSTLICERMIEESGTLDAIQRGVREPFQEHFKR
                     VYLTEDHLLTEYDLA"
     gene            complement(354..>2006)
                     /locus_tag="VI520_03360"
     CDS             complement(354..>2006)
                     /locus_tag="VI520_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HLF36954.1"
                     /translation="QHKLQAYDSLADYNEKHLKDSLPRVLVVIDNVSEFKETYEQHLL
                     DLIAMVRDGRAFGVHFAITAPLISDVHSKLFNILGQRITFTQADPSDYSTIVGRGWAR
                     FNDEPGRGLAVEMLDGRPQPLEFQTGVPAGEPDGEAYRELAQRMATAWEGLVAETPAL
                     AGRRAKPVEPLAEAIDLQTVLPALSKGPAAVSAPVGINDLDREPTVVEFKAKGPHWIV
                     MGPPLTGKTTTLRSLVLSLAHSYSPQQVAMVLVDPSDPARRFFSLGSGNGNTLDKLPH
                     VLATVTDAEQLDAVVKRLVAEFDDNVRGRLKGHPQIFTPVDNSARSIFVIIDHYDDAG
                     LLSRSGVGLPGLAEVGKGKNLHLVLAGTTNITRDSSDELRRRAESARYTLVLQDVEVV
                     RYMGVRGSFNLKKDMPPGRGFLVKAVNASMVQICLPFLDGELGTDPLDDRIEAIRRKY
                     PQAAQWSYRPMDLGPLEAAIGGAAEIAAEQMATVAAEQTESLAELSQLIALQAELAAQ
                     SSGPIAEGSEFASVEVKTDGGRARGKTGDGRGKKPGARGKRG"
BASE COUNT          333 a          667 c          607 g          399 t
ORIGIN      
        1 ggccaggtcg tattcggtta gcaagtgatc ttcggtaagg taaacccgtt tgaagtgctc
       61 ctgaaacggc tcccgaactc cgcgctggat cgcatcgagg gtaccgctct cctcaatcat
      121 ccgttcacag atcagggtgg aaccttcgat gacgcctgca tgctccaggg gtgaccgcag
      181 gctcaatggc ccttcgtcct gccccagcat cagctgcaag gtcccgtcga tgttgaactt
      241 gtcttgaatt tcggcgagta gattggcgac caacaggctg cttcggacgt tgatccgctt
      301 ggacccaatc tcattcggtc ggatatctag agtcaggctg agccgttcca tggttaaccc
      361 cgcttgcctc tggcgcccgg cttcttgccg cggccgtcgc cggttttccc tctagccctg
      421 ccgccgtcgg tctttacttc gacgctggca aactcggaac cttcggcaat cggtccggag
      481 gactgggccg ccagctcggc ctgtagcgcg atcagttggg atagttccgc cagcgattcg
      541 gtctgttcag cagcgacggt cgccatctgt tctgcggcga tctctgccgc gcccccgata
      601 gcagcctcca gcggtcccag atccatcggg cggtagctcc actgggccgc ctgtgggtac
      661 ttccggcgga tggcctcgat ccgatcatcc agcggatctg ttccgagctc gccatccagg
      721 aacggcaggc agatctgcac catggaggcg ttcaccgcct tgaccaggaa accgcgtccc
      781 ggcggcatat ccttcttgag gttgaagctg cctcgcaccc ccatgtaccg aaccacctcg
      841 acgtcctgca gcaccagggt gtagcgcgcc gactcagccc gtcgccgcag ctcgtccgag
      901 ctatcgcggg tgatgttggt tgtgccggcc agcaccaggt gcagattctt gcccttgccc
      961 acctcggcca accccggcag cccaactccg gagcgactca gcagcccggc gtcatcgtag
     1021 tggtcaatga tgacgaaaat cgagcgggcc gagttgtcca ccggggtaaa gatctgaggg
     1081 tgccccttga gccgcccgcg tacgttgtca tcgaactccg cgaccagccg cttgacgacg
     1141 gcatccaact gctccgcgtc ggtgacggtg gccagcacat gcggcagctt gtccagtgtg
     1201 ttgccgttgc cgctgccgag gctgaaaaac cgtcgggccg ggtcggaggg gtcgaccagc
     1261 accatcgcaa cctgctgcgg cgagtaactg tgtgccagcg aaaggacgag tgagcgcagg
     1321 gtcgttgttt ttccggtcag cggcggtccc atcacgatcc agtgcggacc cttggccttg
     1381 aactcgacca cggtcggctc gcgatccaga tcattgatcc ccaccggtgc gctgacggcc
     1441 gccggcccct tgctcagcgc cggcagcacg gtctgcaggt cgatcgcctc cgcgagcggc
     1501 tcgaccggct tcgcccgcct tccggccagc gccggagtct cggcaaccaa tccctcccag
     1561 gcggtcgcca tccgctgggc gagctcccga taggcctccc catccggctc gccagccggg
     1621 acgcccgttt ggaattccag gggctgcggg cggccgtcga gcatctcgac cgccaacccc
     1681 cgccccggtt cgtcgttgaa gcgggcccaa ccgcgcccga cgatcgtgga gtaatcgctg
     1741 gggtcggcct gagtgaacgt gatccgctgt cccaggatgt tgaacagttt gctgtgcacg
     1801 tccgagatca gtggagcggt gatcgcgaag tggacaccga aggcgcggcc gtcccggacc
     1861 atcgcgatca gatccagcag gtgctgttcg taggtctcct tgaactcgga aacattgtcg
     1921 atcaccacca gcaccctggg cagcgagtct ttcaaatgtt tctcgttgta gtcggccagc
     1981 gagtcatagg cctgaagttt gtgctg
//
LOCUS       DATDPQ010000141         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_317486, whole genome shotgun sequence.
ACCESSION   DATDPQ010000141 DATDPQ010000000
VERSION     DATDPQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317486"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..96
                     /locus_tag="VI520_03365"
     tRNA            23..96
                     /locus_tag="VI520_03365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57..59,aa:Val,seq:gac)
     gene            501..2294
                     /gene="thrS"
                     /locus_tag="VI520_03370"
     CDS             501..2294
                     /gene="thrS"
                     /locus_tag="VI520_03370"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HLF36955.1"
                     /translation="MSKPKEPRYEESELYRLRHSAAHVMAQAVQEMFPGLAKLAIGPP
                     IADGFYYDFELPRPLTPEDLEQIERRMREIIGGNHPFAKRVVSAAEARKLFADQPYKQ
                     ELIEGLEAGGVDEYGEPTKAKPEISVYTHDSFTDLCRGPHVEHTGRINPDALKLINVA
                     GAYWRGDERRTMLQRIYGTAWLTSEELEQHLWRLEEARRRDHRRLGRELDLFSISEQV
                     GPGLILWHPRGGLVRKIAEDFCRAEHERAGYQFVYSPHIGKAELWETSGHLDWYKEAM
                     FPPVELEGQQYYLKPMNCPFHVQIYKSKTRSYRDLPQRYAEWGTVYRYERSGVLHGVL
                     RVRGFTQDDAHLFCRPDQMPEEIDRTLAFSLHILRSFGFQDFHAYLATRDPAKAAGSA
                     EQWQAPTQALQSALERAKIPYQIDEGEATFYGPKIDLKLMDALGREWQLTTIQFDFWL
                     PERFGLTYVGEDGQEHQPYMIHRALLGSMERFLGTLIEHYAGAFPVWLAPVQAMVIPI
                     ADRHAEYAGRVASQLREAGLRVEVDDRGERMNAKIRDAQMQKVPYMLVVGDREAQAGQ
                     VSVRPRSEEDLGAMSVEGFLDRAQRDIKARI"
     gene            2442..>2970
                     /locus_tag="VI520_03375"
     CDS             2442..>2970
                     /locus_tag="VI520_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36956.1"
                     /translation="MRTYFLTWVFLLSGFASRADLAGGLLPGADARDFICAPRLQLRH
                     PEFCPQHGPGAKLIELARLGLYPPKPLPTDATDPNLGFLPWDYLRASSGETTIYASEE
                     DAAAGGSGASSLASGFVYLSTYGRAEIDGDTIYATPLGYVRGGSVSALQLPASPGLAF
                     SRTPDRPFGWIVQGGT"
BASE COUNT          574 a          895 c          965 g          536 t
ORIGIN      
        1 ccctctgtat aatcgtgcag cagggcggtt agctcagttg gttagagcgc atgcttgaca
       61 tgcatgaggt cgtaggttca aatcctatac cgcccacaca atcgaatagc tacacgcgac
      121 gaccgggacg agtagtcgga gtgcggtgca cacaggaacg cgggtcccca gactgagaga
      181 cccacggtgc tccggtcgac gaaaaccccc cgcgagcgga gacctgaagg cccgaggccg
      241 agcttggata ggagccagcg aatctaaggc tggccgagct catggcccgg gcagataagg
      301 cgaacggttt agccccgtta ccggctagca aagatggctt gacagcctcg gactcaaggc
      361 agcggttccg agcctgacag ccaatctggg tggaaccgcg tgagcgccgg ctcttgcccc
      421 agtggggcgg gagccgtttg ttttccgaat aggagggagc gcccgcctct gcgtgcgccc
      481 gaaagttaag gaggactgag atgtccaagc caaaggagcc gcgctatgag gagtccgagc
      541 tttaccgcct ccggcactcg gctgcccacg tcatggccca ggcggtgcag gagatgttcc
      601 ccggcttggc caagctggcc atcggcccgc cgattgccga cggcttctac tacgacttcg
      661 agctgcctcg acccttgact ccggaggacc tggagcagat cgagcggcgg atgcgcgaga
      721 tcatcggcgg caaccatccc tttgccaaac gggtggtcag cgcggcggag gcccgcaaac
      781 tgttcgccga tcaaccctac aagcaggaac tgatcgaggg cctcgaggct ggaggcgtgg
      841 acgagtacgg ggagccgacc aaagcgaagc ccgagatttc ggtttatacc cacgacagct
      901 tcaccgatct gtgccgcggc ccacatgtcg agcacaccgg gcgcatcaat ccggatgccc
      961 tgaaattgat caacgtggcc ggggcctact ggcgtggcga cgagcgacgg accatgctgc
     1021 agcgaatcta cgggacagcc tggctaacca gcgaggagct agagcagcac ctgtggcggc
     1081 ttgaagaggc ccgccggcgc gaccatcgcc ggctcgggcg agaactcgat ctcttcagca
     1141 ttagtgaaca ggtcggcccc ggcctgattc tctggcatcc gaggggtggg ctggtgcgca
     1201 agatcgccga ggacttctgt cgtgcggaac acgagcgagc aggctaccag ttcgtgtatt
     1261 cgccccacat cggcaaagcg gagctctggg agacgagcgg ccatttggat tggtacaagg
     1321 aggctatgtt ccctccggtc gagctggagg gccagcagta ttacctcaag ccgatgaact
     1381 gtcctttcca tgtacagatc tacaagagca agacccgttc ttatcgggac ttgccgcagc
     1441 gttatgcaga gtggggaacg gtctatcgct acgagcgcag cggcgtactg cacggagtgc
     1501 tgcgagtgcg cggcttcacc caggacgacg cccacctatt ctgccggccc gatcagatgc
     1561 cagaggagat cgaccgcacc ctggccttca gcctgcacat cttgcgctcc tttggatttc
     1621 aagacttcca cgcctatttg gccactcggg atccggccaa ggcagccggc tcagccgagc
     1681 agtggcaggc gccgactcag gcgctccaat ccgcactcga acgcgccaag atcccatatc
     1741 agatcgatga gggggaggcc actttctatg gccccaagat cgatctcaaa ttgatggacg
     1801 cgctgggtcg ggaatggcag ttgaccacaa tccagttcga cttctggctg ccagagcgtt
     1861 ttggcctgac ttacgttggc gaggatggcc aggaacacca gccctacatg attcaccggg
     1921 ccctgttggg atcgatggaa cgctttctgg ggactctgat cgagcactat gccggcgcct
     1981 tcccggtttg gctggcgccg gtccaggcga tggtgatccc gattgccgat aggcacgccg
     2041 agtatgccgg ccgagttgca agccagctgc gggaggcagg gctgcgggtg gaggtcgacg
     2101 accgaggcga gcggatgaac gctaagatcc gggatgccca gatgcagaag gtgccctaca
     2161 tgctggtggt aggagatcga gaggcgcagg ccgggcaggt ctcggtgcgg ccgcgcagcg
     2221 aagaggactt gggggcaatg tcggtggagg gctttctgga tcgtgcgcag cgagacatca
     2281 aggctcggat ctgaaattaa caaggtgatg ggatggccga ggcactttag gcggctgcaa
     2341 ggtggcccgg agcgctgctg atccggagcc gctccgcctg atatggaccg cctcgggtaa
     2401 aatcgccttc agtgcttggc aatgccagcc agcccctgac gatgcgcacc tacttcctga
     2461 cctgggtttt ccttctgagc gggtttgcca gccgagctga tctcgcgggc gggctgctac
     2521 ccggggcgga tgctcgcgac ttcatctgtg cgccgcggct gcagcttcgc catcccgaat
     2581 tttgtcctca acacggacct ggagccaagc tgatcgagct agcccggctg ggcctctatc
     2641 ctcccaaacc gctgcccacc gatgcgactg atccgaacct gggctttctg ccttgggact
     2701 acctgcgagc aagctcgggg gagaccacga tctacgccag tgaagaggac gcggccgccg
     2761 gagggagcgg ggcctcctcg ctcgcgtctg gattcgtcta cctatcgacc tacggccggg
     2821 cagagatcga tggagacacg atctatgcca ccccgctcgg ctatgtgcgg ggtggttcgg
     2881 tgagcgccct ccagctgcct gcctcgccgg ggctggcctt ttcgcgtacc cccgatcggc
     2941 cgttcggttg gatcgtgcaa ggaggcacct
//
LOCUS       DATDPQ010000142         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1633786, whole genome shotgun sequence.
ACCESSION   DATDPQ010000142 DATDPQ010000000
VERSION     DATDPQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633786"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VI520_03380"
     CDS             <1..492
                     /locus_tag="VI520_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLF36957.1"
                     /translation="FRAGEFGGVITLPIEEIPPPDSGPKEFAEGRFWLVNVGQATIDD
                     PRQPTDYPLQPGIRVLYKVCVHLGCLYKWVPVNDRFECPCHGSKYLKAGTRIDGPANR
                     NLDQYIMEVVDAAGQVLARAEPSMDGREGASVPIPAGAVALRIDTGRRINGAPNTRAG
                     GGL"
     gene            508..2127
                     /locus_tag="VI520_03385"
     CDS             508..2127
                     /locus_tag="VI520_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HLF36958.1"
                     /translation="MRVPLPPLPKFIHLPFLDDLKHKGLKRAVTDLADDWTTRITSGL
                     NMSDVRSVLRGDTPRRPNPRLTPHAEGFWLHMKPTYYHNLMDGLYPTFRLGWLATFFF
                     AWEILTGIYLMVFYTPSPLAAYENMLAILSNVPFGRWMRDTHKLGGELMIIIVVLHML
                     RSFVTGSYKRPRQFTWATGGILLIVTLFVSFFGYLLPWDQLSLWAVTIGASTVGVTPL
                     VGREMDLLLRGGPEIGANGLLRAYLLHVIALPVVGVIALGVHYYKVTHHGFSLPPEAE
                     EVGTDTARKVPMEARVYFMPKVMTRELAYVSVLVLILLAASAFSWGYHAPLEPHADPL
                     VTPLHTTSPWYFLWIQGMMKLGDNFIFGAVIPFMIVLPLIVVWPYLEVGPSRRYGSRR
                     IGLSIAAFSLIGTAALTYMGTPWFAVETSPDQEAIAVLLPQTSPGPLRQADWEQLPYG
                     TYEAGQWAQAPTRTLSRLLQTFDEEVEKAERNPRYSYVDPEGLMIIEDWQADLKKITL
                     RVVWTDGETGKASEFFQVQYLHRFSDYGQGE"
     gene            2131..>2719
                     /locus_tag="VI520_03390"
     CDS             2131..>2719
                     /locus_tag="VI520_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF36959.1"
                     /translation="MKYYILVGMGALVGSVTLFAVMGINEQRRMESFDRSYSSRRIEE
                     GAAIFENNCRLCHGPQGEGTPLGPTLNGAAMFNGERLQAFGWAGTLEDFLRSTISAGR
                     PVPSAGTSYPQQMPTWSEAYGGPMRIDQIESVVGFILNWEERALAGAEPTPVTGEMMG
                     TDMNVVLPPGDAEAGRALAEGTLGCAACHILSPVGP"
BASE COUNT          486 a          839 c          857 g          537 t
ORIGIN      
        1 ttccgggcgg gtgagtttgg tggggtaatc acccttccca tcgaagagat ccccccaccg
       61 gattcggggc ccaaagagtt tgcggagggc cgcttctggc tggtcaacgt gggccaggcc
      121 accatcgatg acccgcggca gcccacggac tacccgctcc agcccggcat ccgggtcttg
      181 tacaaagtgt gcgtccacct gggctgcctc tataaatggg ttcccgtcaa cgaccggttc
      241 gagtgcccct gccacggctc gaaatacctc aaggccggta cccggatcga tgggccggcc
      301 aatcgcaatc tggaccagta catcatggaa gtcgtcgatg ctgcaggcca ggtgctggcc
      361 agagcagagc cttccatgga tggacgggaa ggcgcttcgg tccccatccc ggctggcgcg
      421 gtggcactgc ggatcgacac cggtcggcgc atcaacggtg cgccgaacac ccgggccggc
      481 ggcggtttgt aagcagggag acccgacatg cgcgtcccat taccaccctt gccaaagttc
      541 attcatctcc cgttcctcga tgatctcaag cacaaaggcc taaaaagagc tgtcacggat
      601 ctggctgacg attggacgac ccggatcacg tccgggctca atatgtcgga tgttcgatcc
      661 gtgctgcgcg gcgacacccc ccgccggccg aacccacgcc tgaccccgca tgccgagggc
      721 ttctggctcc acatgaagcc gacctactat cacaacctca tggacggcct ctacccgacg
      781 tttcggctgg gttggctggc caccttcttc ttcgcctggg agatcctcac cggcatctac
      841 ctgatggtct tctacacccc ctcaccgcta gcggcctatg agaacatgct cgctatcctg
      901 agcaacgtgc ccttcgggcg ctggatgcga gatacccaca agctgggcgg cgagctcatg
      961 atcatcatcg tggtgctgca catgctgcgc tcgtttgtga ccggctccta caaacggccg
     1021 cgccagttca cctgggcaac cggcggcatc ttgttgatcg tcacgctgtt cgtctccttc
     1081 ttcggatacc tattgccctg ggatcagctc agcctgtggg cggtaaccat cggtgcctcg
     1141 acggtcggag tgaccccctt ggtcggtcgt gaaatggatc tgctgctgcg cgggggtccg
     1201 gagattggag caaacggatt actgcgcgct tacttgctgc atgtgatcgc gctgccggtg
     1261 gtcggagtca tcgccttggg cgtgcattac tacaaggtga cccaccacgg cttcagcctg
     1321 ccccccgagg cagaggaggt ggggacggat accgctcgca aagtgcccat ggaggcgcgg
     1381 gtttatttca tgcccaaggt catgactcgc gagttggcct atgtctcggt gttggtcttg
     1441 atcctgctgg cggcttcggc gttttcctgg ggttaccatg cgccgctgga gccgcacgcc
     1501 gatccattgg tgacgccgct gcacaccacc tcgccctggt acttcctgtg gatccaggga
     1561 atgatgaagc ttggagacaa ctttatcttc ggcgcggtca ttcccttcat gatcgtactt
     1621 cctctgatcg tggtttggcc ctacctggaa gtggggccga gccgccggta tgggagccgg
     1681 agaatcgggc tctcgatcgc tgccttcagc ttgatcggca cggcggcgct gacctacatg
     1741 ggtaccccct ggtttgcggt cgagacctcc cccgaccagg aagccatcgc agtcctgctg
     1801 cctcagacga gtcccggacc gctccgacaa gcggactggg agcagctccc ttatgggacc
     1861 tacgaggccg gtcagtgggc ccaggcgccg acccggacgc tgtccaggct gctgcagacc
     1921 tttgacgagg aagtcgagaa ggcggagcgc aatccacgct acagctacgt agatcctgaa
     1981 gggttgatga tcatcgagga ttggcaagcc gatctaaaaa agatcacctt gcgggtagtg
     2041 tggacggatg gcgagaccgg caaggccagt gaattcttcc aagttcaata ccttcaccgc
     2101 ttcagcgact acggccaggg ggagtagttc gtgaaatact acatcctggt cgggatggga
     2161 gcgctggtcg gttcggtgac gctgtttgcc gttatgggaa tcaacgagca gcggcgcatg
     2221 gagtcgttcg atcgttccta cagctcacgc cggatcgaag agggcgcggc gatcttcgaa
     2281 aacaactgcc ggctgtgcca tggtcctcag ggggagggca cacccctggg ccccacccta
     2341 aatggcgcag ccatgttcaa tggtgagcgg ctgcaggcgt tcgggtgggc tggcacgctg
     2401 gaggatttcc tgcgcagcac aatctcggcc ggccggcccg tgccgagcgc ggggaccagc
     2461 tatccccagc aaatgccgac ctggagcgag gcctatgggg ggccgatgcg aattgatcag
     2521 attgagtcgg tagtgggttt catcctgaac tgggaggagc gggcgctggc gggggccgag
     2581 ccgaccccag ttaccggaga gatgatgggc accgacatga atgtcgtact tcccccgggc
     2641 gatgcggagg ccggtcgggc gctggcggag ggaaccctcg gctgcgcggc ctgccacatc
     2701 ctgtcgccgg tcggcccgc
//
LOCUS       DATDPQ010000143         3671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1370839, whole genome shotgun sequence.
ACCESSION   DATDPQ010000143 DATDPQ010000000
VERSION     DATDPQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370839"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /gene="priA"
                     /locus_tag="VI520_03395"
     CDS             <1..1413
                     /gene="priA"
                     /locus_tag="VI520_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HLF36960.1"
                     /translation="ERYDTWRRARAGSLSVIVGPRSALFAPLQDIGLIVVDEAHDESY
                     KEHGQAPRHHARDTALEYARLCSAVCLLGTATPDIVTRYQADRGRLTRLALPLRILGH
                     RQVLQQQAARLGLQSSYQSAGPTAETIDLPPVRVVDMRHELRAGNRSIFSRALHRALE
                     STLDSGQQAILFLNRRGTATYVFCRDCGFVLRCSNCDSPLTFHGARELLLCHHCGRDR
                     RMPERCPNCGNPRIKQFGAGTQRVQAEVERLFPGVRTIRWDWDTTRSKGAHDLILQTF
                     AAQQADVLIGTQMVAKGLDLPLVTLVGVISGDVGLYLPDYRAPERTFQVLTQVAGRAG
                     RSLLGGQVILQTYDPDHYAIQAAAAHDYPAFYQAELALRRELGYPPFRRLIRLEFRDS
                     AEARAQKEAERLAGLLAARIQELGLRRTDLIGPVPAFFRRVRAEYRWMVVIRAPDPLK
                     LLPEDLPRGWIVDVDPVSLL"
     gene            complement(1429..2121)
                     /locus_tag="VI520_03400"
     CDS             complement(1429..2121)
                     /locus_tag="VI520_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HLF36961.1"
                     /translation="MPGPRWYPAVVAVFSSALIVSNIIAVKLVDLFGLTLPAGVVLFP
                     IVYIVGDVLTEVYGFGRARQAIWIGFGCNLLAVGSIWIAGRLPAAAFWNAGVYPGPAE
                     AGQAYQAILGFSGRLIVASFLAYLVGEFLNAFVLAKLKLATEGRMLWLRTIGSTLVGQ
                     AADSAVFITAAFWGILPAAALSSAVVAQWLVKSGYEALATPLTYLVVNALKRVEGVEV
                     FDRTTDFRPVAF"
     gene            complement(2122..2532)
                     /gene="queF"
                     /locus_tag="VI520_03405"
     CDS             complement(2122..2532)
                     /gene="queF"
                     /locus_tag="VI520_03405"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HLF36962.1"
                     /translation="MTEASDLAGLTLLGHGGGPSKRLETFPNRHPERRYVVRLETDEF
                     TCVCPATEQPDFATIRVEYIPDKVILESKSFKLYLWSYRNEGVFHEHVTNKILDDLVS
                     ALEPHWCQVRGAFNVRGGIAITVEAEHGSREAPA"
     gene            2648..3055
                     /locus_tag="VI520_03410"
     CDS             2648..3055
                     /locus_tag="VI520_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36963.1"
                     /translation="MRPLAGQIPGASPSVLVVDDDSSYCTIIRELLVRNGYRVRLANG
                     AEQAFDLLQSERPDLILADLMMPEIDGLTFIRQLRATPAQARIPTIVVSALVLAKERV
                     AAAQAGADAFVAKPFSINQLRAVINTVMAPIRG"
     gene            complement(3107..>3671)
                     /locus_tag="VI520_03415"
     CDS             complement(3107..>3671)
                     /locus_tag="VI520_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF36964.1"
                     /translation="ERLRSRFEWGLAADIQPPDFETRVAILRSKAERAGRTVQHEHLK
                     LIARRAQNNIRELEGALTRAFALADLTGAELGEATIEASLSELASKTSRLTPGRIIAA
                     VAQQFQVPEAELCGPRRTRQLALPRQVAMYLIREETDESLPRIGQALGGRDHTTVMYG
                     YERIANRLETDEGLRRQVIAIRSSLFQ"
BASE COUNT          654 a         1193 c         1162 g          662 t
ORIGIN      
        1 gagcgctatg acacctggcg ccgggcacgg gccggttcgt tgtcggtcat cgtgggtccg
       61 cgcagcgcgc tcttcgcacc gctgcaggat atcgggctga tcgtggtcga cgaggcgcac
      121 gatgagtctt ataaggaaca cgggcaagct cctcggcatc atgcccgcga caccgccctg
      181 gaatatgccc ggctctgctc cgcggtctgc ctgctgggaa ccgcgacccc cgatatcgtg
      241 actcgctacc aggcggatcg gggcaggctc acccggcttg cccttccgct tcggatcctc
      301 ggacaccgcc aagtgctgca gcagcaggct gcccggctcg ggctgcagag cagctaccag
      361 tcggccggtc cgactgccga gacgattgac ttgcctccgg tacgagtcgt cgacatgcgc
      421 cacgagctgc gggcgggcaa tcgatcaatt tttagccggg ccctccatcg ggcgttggag
      481 tccaccctgg actccggcca acaagccatc ctcttcctga accggcgggg gactgcaacc
      541 tacgtgtttt gccgggactg cggatttgtg ctgcgctgct cgaactgcga ctcgccgctg
      601 actttccacg gtgcgcggga gctcctgctc tgtcatcatt gcggaaggga tcgccgtatg
      661 ccggagcggt gccccaactg tggcaacccc cggatcaagc agttcggagc cggaacccag
      721 cgggtgcagg cggaggtcga acgactgttt ccgggggtcc ggaccatccg ttgggattgg
      781 gacaccaccc gcagcaaagg ggctcacgac ctgatcttgc agaccttcgc cgctcagcag
      841 gcggatgtgc tcatcgggac ccagatggtg gctaaggggc tcgatctgcc gctggtgact
      901 ctggttggcg tgatttcggg cgacgtcggt ttgtacctgc cggactatcg ggcgcccgag
      961 cgtacctttc aagtcctcac ccaggtcgcg ggccgcgccg gacgcagtct gctcggaggc
     1021 caggtcatcc tgcagacgta tgacccggac cactacgcga tccaggccgc cgcggctcac
     1081 gactatcccg cgttctacca agctgaactt gctcttcgaa gggaactcgg atatccaccg
     1141 ttccgccggc tgatcaggct ggagtttcgg gattccgccg aagccagagc tcagaaggag
     1201 gccgagcggt tggccgggct gcttgccgcc cggattcagg agctcggatt gcgccgcacc
     1261 gacctgatcg gaccggtgcc ggccttcttc cgccgggtgc gcgccgaata tcgctggatg
     1321 gttgtgatcc gagcccctga cccgctcaag ctgcttccgg aggatctgcc ccgcggctgg
     1381 atcgtggacg tggacccggt ctccttgctg taaacggccg gggcgagttc agaaagcaac
     1441 ggggcggaag tcggtcgtgc gatcgaatac ctcgacccct tcgacccgct tgagggcgtt
     1501 gaccaccaga taggtcagcg gggtcgccag cgcctcatac ccgctcttga cgagccattg
     1561 tgcaaccacc gccgagctca gcgcggcagc ggggaggatt ccccagaacg cggcagtaat
     1621 gaaaactgcc gagtccgcgg cttggcccac cagagtggag ccgatggtcc gcagccagag
     1681 cattcgtcct tcggtcgcca gcttgagttt ggccagcacg aaggcgttca agaactcccc
     1741 caccaggtag gccaggaatg aggccacgat caagcgcccg ctgaacccta agatcgcctg
     1801 ataggcctga ccggcttctg ccggtcccgg atagaccccg gcgttccaga acgcggcggc
     1861 cggcaggcga ccggcgatcc agatcgagcc gactgccagc agattgcagc caaacccgat
     1921 ccagatggcc tggcgggctc ggccgaaccc gtacacctcg gtcaagacat ctcccacgat
     1981 gtagacaatt ggaaagagga ccacccccgc cggcagggtg aggccgaaga gatcgacgag
     2041 cttgaccgcg atgatattgg aaacgatcag ggccgagctg aagacggcca cgaccgccgg
     2101 gtaccagcgg gggccgggca tctaggccgg cgcctcccgg ctgccatgct cggcttccac
     2161 cgtgatcgca atcccgcctc gcacgttgaa ggccccccgc acctggcacc aatgcggctc
     2221 cagggccgac accaggtcat cgaggatctt gttggtcacg tgttcatgga aaacgccttc
     2281 attgcgatat gaccacaggt agagcttgaa cgacttggat tcgaggatca ccttgtcggg
     2341 gatgtattca acccggatgg tcgcgaagtc cggctgctcg gtcgcggggc agacacaggt
     2401 gaattcgtcc gtttccagcc gaaccacgta tcggcgctcg ggatgacggt tcgggaaggt
     2461 ctcgagccgc ttgcttggac cgcccccgtg tcccaataac gtcagacccg ctaggtcaga
     2521 agcctcggtc actggcacct ccctcgatcc ggccgggtgg cgcgaagtgc accgtcccgt
     2581 gtagactggc gaattgtaac gcgccgaatt ctcggcatga tccttgcagc agctgctaga
     2641 gactgatatg aggccactgg cgggtcagat tcctggagct tccccaagcg tactggtggt
     2701 cgacgacgat tcgtcgtact gcacgattat ccgcgaattg ctggtgcgca acggataccg
     2761 agtccggctg gcgaacgggg cggagcaggc cttcgatcta ctgcagagcg agcgtccgga
     2821 cctgatcctg gccgacctga tgatgcccga gattgatggg ctgaccttta tcagacagct
     2881 gcgggcaacc ccggctcaag ccaggatccc gacgatcgtc gtcagcgccc tggtgctggc
     2941 caaggagcga gtcgcagcag ctcaagccgg ggccgacgcc tttgtggcga agcccttctc
     3001 aatcaatcag ctgcgggccg tcatcaatac cgtgatggcc ccgatccggg gttaaagaaa
     3061 acgccgccaa cttggcggcg ggtggaaact ctccgctcca tcccactcac tggaacagtg
     3121 aactccgaat ggcgatgacc tgcctacgca gcccttcgtc agtctcaagc cggttcgcaa
     3181 ttcgctcata gccatacatc accgtggtgt ggtcgcggcc gcccagcgcc tggccaatcc
     3241 gggggagcga ttcatcggtt tcctcccgga tcagatacat cgccacttgc cgcggcaggg
     3301 cgagctgccg agtccgccgc ggtccgcaca gttcggcttc cggcacttgg aactgctgtg
     3361 ccacggcggc gatgatcctt ccgggggtca gccggctggt cttggaggcc aactccgaaa
     3421 gggaggcctc gatcgtcgcc tcccccaact cggccccggt gagatctgcc agggcgaagg
     3481 cccgggtcag tgccccttcc agctcccgaa tgttgttttg ggctcggcgg gcaatcagct
     3541 tgagatgctc atgctgaacg gtccggccgg cccgctccgc cttggagcgc aggatggcga
     3601 ctcgggtctc gaaatcaggt ggttggatgt cggctgccag tccccactcg aaccgtgagc
     3661 gcagccgttc c
//
LOCUS       DATDPQ010000144         4930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_577438, whole genome shotgun sequence.
ACCESSION   DATDPQ010000144 DATDPQ010000000
VERSION     DATDPQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577438"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VI520_03420"
     CDS             <1..1392
                     /locus_tag="VI520_03420"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HLF36965.1"
                     /translation="EAIKHILFRWPSVAVLVLTSLTSDHDVFAAVKAGALGYILKDTE
                     PEELVQAIKQVHRREPTLHPKIARILLDEVTQSAPEGELAAVLTAEEIGVLRLLTMGR
                     DTSQIAGGVAKSAEQVRIHINSILSKLHLASRTEAVLLALREGLARPDEASPKYLTLL
                     LEAVRQERKELSRDDSSPSEAPTEPMVESIGLADLIREHRQFELELTLAGKIQSSFLP
                     RALPDPPGWQFAAKLIPARETSGDFYDLIQLPDSRVGLVIADVTDKGMGAALYMALTR
                     TLMRTYAQEGAAKPHQVLQEVNGRILADTEGGYYVTLFYGILDLKTGVLDYSNAGHPP
                     GVLVRGPETNRLKVLGRTGPPLGVTKQAPWESSVISIQPGNHLILYTDGISEARDSSS
                     QLFGIERVIQAALASSARSASETQDMILKLVERFAGEQPRADDIALVVASRILAESDT
                     TAFHPVLPPLARD"
     gene            complement(2237..3166)
                     /locus_tag="VI520_03425"
     CDS             complement(2237..3166)
                     /locus_tag="VI520_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF36966.1"
                     /translation="MRLTETPFVIHPSTRIGHVHLTVADLDRQIAFYQQKIGFQLHWR
                     EGERAGLGAGRTDLLNLTEDRRARRVRGTTGLYHFAILLPNRRELARALARLFSLRYP
                     NYPTDHVMTETTYLDDAEGNGIELYADTPERGTWSFSGDSFQVVDAQGVVRSGRDPLD
                     VERLLRELEPGDRLDQPMPEATTIGHVHLHVADLADANRFYCDLLGFEVQGMSAAMGA
                     SFVSAGGYHHHLGLNTWVGEGAPPPPPDALGLRHFEIVLPDQAELTRLAERVRAANLV
                     PEDTEAGVLIRDPSRNGVLLASGSNSHGLRPDA"
     gene            3203..4003
                     /locus_tag="VI520_03430"
     CDS             3203..4003
                     /locus_tag="VI520_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HLF36967.1"
                     /translation="MDNDDRLIGRILSRREALSVLGVTSAAVLVGCAPGGSEPEPTSQ
                     VSTQALSSTETEAATAEPAADTVPTCVVRPEMTEGPYFVDEQLNRSDIHSDPSDGSVM
                     EGALFELTFRVSEVSNSACSPLEGVFVDIWHCDALGVYSDARDQGFNTVGQKFLRGYQ
                     LTDANGLARFTTIYPGWYFSRAVHIHFKIRSELSANSSYEFTSQLFFDDAQSDQVFAG
                     EPYASKGERIIRNADDRIFQEGGSQLLLEVSQRDSGYAATFDIGLQLA"
     gene            complement(4072..4701)
                     /locus_tag="VI520_03435"
     CDS             complement(4072..4701)
                     /locus_tag="VI520_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLF36968.1"
                     /translation="MTDKYLESLLAQNEKLVLTARQHTFILLSAIAAEILVIGLIIAG
                     VTALTALLLPLAAFGYLLLILPIAKGTYDFLNWRQLQYVITNRRVIQLSGIFSKSVTD
                     SSLEKVTDVQMSQSTFGRIFNYGDIEILTASEQGANNFRRIANPVRFKTAMLDAKEQM
                     GYGEGPGAARTSPSDDIPAIIAKLDGLRAKGVITEQEFQDKKRDLLKKM"
BASE COUNT          924 a         1507 c         1626 g          873 t
ORIGIN      
        1 gaggccatca agcacatact atttcgctgg ccttcggtag ctgtactggt gctcaccagc
       61 ctcacatctg atcacgacgt ttttgcggcg gtcaaggcgg gagcgctggg gtatatcctg
      121 aaggataccg agccggagga gttggtgcag gccatcaagc aagtccatcg gcgagaaccg
      181 accctgcacc cgaagatcgc gcggattctg ctcgatgagg tcactcagag tgcccccgag
      241 ggagagctag ccgccgtgct gaccgcggaa gagatcggag tgctccggct cttgaccatg
      301 gggcgggata ccagccagat tgcgggcggg gtggccaagt ccgccgagca ggtccgaatc
      361 cacatcaata gcattctttc caagctccat ctggcgagcc ggaccgaggc cgtattgctc
      421 gcgttacgtg aggggttggc ccggcctgat gaagcgtctc caaagtacct taccctgctg
      481 ttagaagccg tgcggcagga gcgcaaggag ctgagccggg acgattcctc tcccagcgag
      541 gctccgaccg aacccatggt tgaatcgatc gggctggccg atctcatccg agaacaccgg
      601 cagttcgaac ttgagctcac cctagccggc aagattcaat ccagctttct accgcgggcg
      661 ctgccggacc caccgggctg gcagtttgcg gcgaagctca ttccggctcg cgagacttcc
      721 ggggatttct acgacctgat tcagctcccc gattcgaggg ttggattggt cattgccgac
      781 gttaccgaca agggcatggg tgccgcgctc tacatggctt tgacccgaac cctgatgcgg
      841 acgtatgccc aggagggcgc agccaaaccc caccaagtcc tccaggaagt caacggccgg
      901 atcctggccg atacagaggg cgggtactac gtcacgctgt tctatgggat tctcgatttg
      961 aagaccggag tcctcgatta cagcaatgcc ggtcacccgc cgggcgtgct ggtgcggggg
     1021 ccggaaacca accgactgaa agttctgggc cgaaccggcc cacccttggg ggtcacgaaa
     1081 caggctccgt gggaatcatc ggtcatcagc attcagccgg gaaaccacct catcctgtac
     1141 acggacggga tttccgaagc gcgggatagc agcagtcagc tcttcggcat cgaacgggtg
     1201 atccaagctg cgctcgccag ttcggctcgt tcggcttccg agacccagga catgatcctc
     1261 aagcttgtgg agcgctttgc cggcgaacag ccgcgggcgg acgacatcgc cctggttgtc
     1321 gcctcgcgaa ttctggccga gagcgacacg accgcattcc atcccgtctt gccacccttg
     1381 gctcgcgatt gacaaccgat taggataaga gggcaggcaa cttacggata aagttggtat
     1441 caggaggtag ccatggcgaa agcacggtcg aagaagcagg gtggcgggcg gaagactgct
     1501 tccaagcgca cggtaggcaa gtcggccaag cgggtcaaag ccgcgccgcc gaaaaagagt
     1561 tctgcggctc cggtcaagcg gtctgcgccg gcgagcaaac cgggcgccgc tcagcggctc
     1621 gaggcacttg aaagtcgcct ggagcagctg accaaacagc tgtcatcgct ggtgcgtcgg
     1681 gccggtccgc cgggtccgcc cggcccgcgc ggcaaggcag gtgaacccgg cccggttggt
     1741 gcgatgggac cgacgggtaa caagggggaa cccggcgttc cgggcgcagt gggtccgaag
     1801 ggagacatgg gccttcaagg atcacacggt cttaccggat cgagagggga aactggcgtc
     1861 gccgggatcc aaggtccccc gggcccgaag ggggacacag gtgcccaggg gccggctgga
     1921 agggacggag ttaagggtga gactggaatg gccggcactg ctggcctgaa gggcgagacc
     1981 ggttcccagg gtccgcccgg gcctgccggg ccacgagggg agcctggccc gcagggtcca
     2041 tctgggccta agggcgacgc gggggccgtc ggcccgcccg gctcggctgg ggcgaaaggc
     2101 gaatccggtc ctcaggggcc gcctgggccc aagggagacc gaggaccgca aggtccagcg
     2161 ggtcctgccg ggagctcaac gaccactccg ccgccatccg gcagctgaag tgccgacgtc
     2221 agctccgccg ctcgagtcaa gcgtctggcc gtagaccgtg tgagttagat ccactcgcaa
     2281 ggagaacccc attgcgagat ggatcgcgga tcagcacccc agcttcggtg tcttccggga
     2341 ccaggttggc tgctcgcact cgttccgcga gccgggtgag ctcggcctga tccgggagca
     2401 cgatctcaaa gtggcggagg ccgagcgcgt caggcggggg agggggagcg ccctcgccta
     2461 cccaggtatt caggccgagg tgatgatggt atccgccggc cgagacgaag cttgccccca
     2521 tcgccgcaga catcccctgg acctcgaacc caagcaggtc gcagtagaaa cggtttgcgt
     2581 ccgcaaggtc tgcgacgtgc agatggacgt ggccgatcgt ggtggcttcc ggcatcggct
     2641 ggtctagccg gtcgccgggc tcgagctcac gcagcagccg ttcgacgtcg agcggatccc
     2701 gacccgagcg caccacgccc tgcgcatcca cgacctgaaa gctgtctccg ctgaaagacc
     2761 aggtgccgcg ctcgggcgtg tcggcgtaaa gctcgattcc gttgccctcg gcgtcgtcca
     2821 gataggtcgt ttcggtcatg acgtggtcgg tgggatagtt cggatagcgc agggagaata
     2881 ggcgagcgag agcccgggcc agctcgcggc ggttcgggag caggattgcg aagtgataga
     2941 ggccggtcgt accgcgcact cggcgggcgc ggcggtcctc ggtcaggttg agcaggtcgg
     3001 tgcggccggc gcccagcccg gcccgttctc cctctcgcca atgaagctgg aagccgattt
     3061 tctgctggta gaaggcgatt tgccgatcca ggtccgccac ggtcaggtgg acgtgcccga
     3121 tccgggtgga gggatggatg acgaagggag tttcagtcag tcgcataggg tacattgtaa
     3181 aatacggcgg aggtcaaacg acatggacaa cgacgatcga ctgatcggcc ggatcttgag
     3241 ccgacgtgag gcactgtcgg tgcttggggt aaccagcgcc gcggtgctgg tcgggtgcgc
     3301 gccgggcggc tcggaacccg agccaacgag ccaagtatcc acccaagcgt tgtcctccac
     3361 tgaaaccgag gcggccaccg cggagccagc cgcggacaca gttcccacct gtgtcgttcg
     3421 gccagaaatg accgagggcc cctacttcgt agacgagcag ctcaaccgat cggatatcca
     3481 ctccgatccc tcggacggat cggtgatgga gggcgccctc ttcgaactca cctttcgcgt
     3541 ctcggaggtc tccaattctg cctgctctcc gctggaaggg gttttcgtcg acatctggca
     3601 ctgcgatgct ttgggcgtgt attcagatgc ccgagaccag gggttcaaca cggtcgggca
     3661 gaagtttctc cgcggctatc aactgaccga tgccaacggg cttgcccgct tcaccacgat
     3721 ctatcccggc tggtacttca gccgagcggt gcacatccac ttcaagatcc gaagcgagct
     3781 gagcgccaac agcagttacg agttcacctc tcagttgttc ttcgacgacg cgcaatcaga
     3841 tcaggtcttt gcaggggaac cgtacgcgag caagggggaa cgcattatcc ggaacgccga
     3901 cgaccgtatc ttccaggagg gaggctccca gctgctgctg gaggtcagcc agagggattc
     3961 gggctacgcg gcgaccttcg atatcgggct gcagctggcc tagcccggtc ccgggtctcg
     4021 ggcccgcctg cggccgttgg agccggcctt gcaatgctcc ggccggcggg actacatctt
     4081 cttgagcagg tcgcgcttct tgtcctgaaa ttcctgctcg gtgatcacgc ccttcgctcg
     4141 gagcccatcg agcttggcga tgatcgccgg gatgtcgtcg gagggggacg tccgggcggc
     4201 tccgggcccc tccccatatc ccatctgttc cttggcatcc agcatggctg ttttgaagcg
     4261 gaccgggttg gcgatccgcc gaaaattgtt ggcgccttgc tcgctggcag tcagaatctc
     4321 gatgtcccca tagttgaaga tccggccgaa ggtggactgc gacatctgca cgtcggtcac
     4381 cttctcgagc gaggagtcag tgacgctctt gctgaagatc cctgagagct ggatcacccg
     4441 ccgattggtg atgacgtact gcagttggcg ccagtttagg aaatcatacg tccctttggc
     4501 gatcggcaag atcaagagca ggtagccgaa ggctgccagc ggcaggagca aggcggtcag
     4561 ggctgtcacg ccggcgataa tcaatccaat caccagaatc tcggctgcga tcgcgctcag
     4621 caggatgaag gtgtgctggc gggccgtgag cacgagtttc tcgttctgag ccagcagact
     4681 ctcgagatac ttgtcggtca tggtaggcaa ctcctaacga ggcggccgag tcgaccccag
     4741 gccggctcaa ccgaatggta tcatgcgccg ccacgcccat gtacagaacc agttgacggg
     4801 caaatcgcac tgatgtcctc cgaccctccc ggccgcggcc cgcaagtccg agcgctggcc
     4861 tccttcctcg ggctccagcg gccgatggtc gtcctgctca cgatggtcgt cttgcttggc
     4921 ctgggcgagc
//
LOCUS       DATDPQ010000145         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_840949, whole genome shotgun sequence.
ACCESSION   DATDPQ010000145 DATDPQ010000000
VERSION     DATDPQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840949"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VI520_03440"
     CDS             <1..733
                     /locus_tag="VI520_03440"
                     /inference="COORDINATES: protein motif:HMM:NF044599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36969.1"
                     /translation="DLCHSDEIKGSPFFNIFANVVLRPFPRGDVLALIEGNLGDAGVA
                     SSQEEKGFVQKLGGGHPFFLQMAGYYLVEGKAQGLAGEPLTAFLEAQFAEQADPHFTY
                     SWSHSSESEKITLLTILGLSQSKPSKKTIASLENLSRMHNRAKLDLISLVKRGLLEET
                     EGVFGLFSPSFGRWIGREVTAEPEEEESTADVEAWLAEGGLERLQPVRGALPKFKKRY
                     WPLMGEVLKELSFELIAGVAMGKLL"
     gene            complement(746..2071)
                     /gene="yqeC"
                     /locus_tag="VI520_03445"
     CDS             complement(746..2071)
                     /gene="yqeC"
                     /locus_tag="VI520_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HLF36970.1"
                     /translation="MLLKDALRIEPGQSVAFTGAGGKSAAIRKLAGELTRALITTTTK
                     LGRGQADLAQHHLIDPNGGELGRIAQLLEQFGTVLVTGPLSEAEEKWTAPARETLETL
                     SRIAAQTAAPLLIEADGARARSLKAPAEHEPVVPDFTNTVVPMAALDVLGQPIDSPAV
                     HRPERVAELLGFEPSHNLGPPEIARLLSSAAGGLKGVPSDAQVRVLMNKVDPSRLAAG
                     RDIAGELLENPGIQAVVLAAVQAQNAVMEVHGRVAGIVLAAGGSSRLGQPKQLVEFRG
                     RPLVWHSVRVGLEAGLSPLVVVIGAAGEQIRRALEGQSVMLIDNPDWEAGQSSSVRLG
                     ISQVEAGIEAAIFLLADMPLMDAELIRQVVRAHRATLAPLVAPRVAGRRANPVLFDRV
                     TFPDLHQLTGDQGGRSLFERYEPAWVEWSESAMLDLDTPEDLERLRKLE"
     gene            complement(2075..>2382)
                     /locus_tag="VI520_03450"
     CDS             complement(2075..>2382)
                     /locus_tag="VI520_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36971.1"
                     /translation="VLRAPKSGVFEGRVQLADRVRQGDLLAVVGSAELRAPFEGIVRG
                     LLHDGVEVSKGLKVGDLDPRGDPALCRLISDKALAVGGGVLEALLTRPEIRRQLSGG"
BASE COUNT          438 a          804 c          745 g          395 t
ORIGIN      
        1 agacctgtgc cattccgacg aaatcaaggg ctcgccattc ttcaacatct ttgccaacgt
       61 tgtgctgcgg ccattcccgc ggggcgacgt actggctttg attgagggca acctggggga
      121 cgccggagtc gcttcctccc aggaagagaa gggcttcgtt cagaagttag ggggcggaca
      181 tcccttcttc ctgcagatgg cgggatacta tctggtggag gggaaggcgc aaggcttggc
      241 aggcgagccg ttgacggcct tcctggaggc ccaattcgcc gagcaggccg atccacactt
      301 cacgtattct tggtcgcaca gctcggagag cgaaaagatc acgctgctga cgatcctggg
      361 cctgagccag tcgaagcctt ccaagaagac catcgcaagt ctggagaatc tctcccggat
      421 gcacaatcgg gcaaagctcg acctgatttc gctcgtaaag cgcggcctct tggaggaaac
      481 cgagggagtg tttgggctct tctcgccgag ctttggtcgc tggatcggcc gggaggttac
      541 cgccgaaccc gaagaggaag agtccaccgc ggatgtcgag gcatggttgg ccgaaggcgg
      601 cctcgagcgc ctgcagccgg tcagaggggc actcccgaag ttcaagaaga ggtactggcc
      661 gctgatgggc gaggtgctga aggagctgtc cttcgaactc atcgccgggg tggccatggg
      721 aaagttgctg taagcacccc gccggtcact cgagcttccg cagacgctct aagtcctccg
      781 gagtatcgag atcgagcatc gccgactcgc tccattccac ccaggccggc tcatagcgct
      841 cgaacagaga gcggccgccc tggtcgcccg tcagctggtg gaggtctggg aaagtgaccc
      901 ggtcgaagag gaccggattc gcccgccggc cggccacccg gggagccacc aagggtgcca
      961 aggtcgcccg atgggcgcgg acgacctgac ggatcagctc cgcatccatc agtggcatgt
     1021 cagccagcag aaagatggcg gcctcaattc cagcctcgac ttgtgatatg ccgagccgca
     1081 ccgagctgct ctggcccgcc tcccagtctg ggttgtcgat cagcatcacc gattggccct
     1141 ccagggcccg ccggatctgc tcccctgctg ccccgatgac caccaccaag ggactcagcc
     1201 ccgcctcgag tccgacccgc acggaatgcc acaccagcgg ccggccgcga aactcgacca
     1261 gctgtttggg ctgacccagc cggcttgaac cgccggccgc caatacaatt cccgcaacgc
     1321 ggccatgaac ttccataacg gcgttttgcg cctgcaccgc cgcaagcacc acggcctgaa
     1381 ttcccggatt ctcgagcaat tcgccggcga tgtcccgccc ggccgccagc cgcgacgggt
     1441 cgaccttgtt catgagcacc cgcacctgag cgtcggatgg gactcccttt agtccgcccg
     1501 cagcggagct gagcaggcga gcaatctccg ggggtccaag gttgtgtgaa ggctcgaaac
     1561 cgagcagctc ggcgacccgc tcgggtcggt ggacggccgg agagtcgatc ggctgaccca
     1621 gcacgtcgag cgccgccatc gggacgactg tgtttgtgaa atctggcaca accggctcgt
     1681 gctcggcggg agccttgagc gatcgggccc gcgccccgtc ggcttcgatc agcaggggcg
     1741 ccgcggtctg cgcggcaatc cgagagaggg tctccagggt ctcacgggcc ggcgcggtcc
     1801 acttttcctc cgcctccgac agcggacccg tcaccagcac ggtgccgaac tgttccagca
     1861 gttgagcgat tcgccccagc tcgccaccgt tcggatcgat caagtggtgc tgagccaggt
     1921 cagcctgccc gcggcccaac ttggtggtgg tggtaatcag cgcccgggtg agctcgccgg
     1981 ccaacttgcg gatggcagcc gatttgccgc ccgcgccggt gaaggcgacg ctctggccag
     2041 gctcgatccg cagcgcatct ttgagcaaca ccggtcaccc gcccgaaagc tgacgccgaa
     2101 tttccggccg agtcagcagg gcttccagca ctccgccgcc cacggccagc gccttatccg
     2161 aaatcaggcg gcagagagcc ggatcgcccc gcgggtcaag gtcaccgacc ttgaggccct
     2221 tcgaaacctc taccccgtcg tgcaacaggc cgcgcacgat cccctcgaag ggcgcccgca
     2281 gctcggccga accaaccacg gccaacagat caccttgccg gactcgatcg gcaagctgta
     2341 ctctgccttc gaatacgccc gacttcggcg cgcgcagcac cc
//
LOCUS       DATDPQ010000146         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_577487, whole genome shotgun sequence.
ACCESSION   DATDPQ010000146 DATDPQ010000000
VERSION     DATDPQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577487"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VI520_03455"
     CDS             complement(<1..281)
                     /locus_tag="VI520_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36972.1"
                     /translation="MKVPLSWLREFVEIEVSIMELAQRLTFAGLEVEEIRHVGLPFEG
                     ASPATRQETRVWGIAWDPGRIVVGEIREVMPHPNADRLVLTRLYDGERE"
     gene            complement(296..1321)
                     /gene="pheS"
                     /locus_tag="VI520_03460"
     CDS             complement(296..1321)
                     /gene="pheS"
                     /locus_tag="VI520_03460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLF36973.1"
                     /translation="MLDRLGQLEREALAQLEAIRTSADLQTWKVNHLGRSALLAQILD
                     GLRELPKDQRPAVGRRANEVKAGLEQAFAAREEGLRREELDRSLAEQRLDVTLPGRPP
                     IRGRLHPASRTLREIIHIWAEMGFQVYRSREVETDEYNFGLLNMPRHHPARDMLDTFY
                     TTDPEVLLRTHTSPGQIRVMRERAPEPIRVVLPGMVFRYEQITARSEIQFNQVEGLAV
                     GHHITLADLKGTLSAFARRTFGQNVRIRFRASYFPFTEPSAEMDIECFVCGGAGCPVC
                     KRTGWLEILGSGMVHPTVLENGGYDPELFSGFAFGMGPERITMLKHGIEDIRYFWAND
                     LRFLAQF"
     gene            complement(1445..3418)
                     /locus_tag="VI520_03465"
     CDS             complement(1445..3418)
                     /locus_tag="VI520_03465"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HLF36974.1"
                     /translation="MSTPRRLYVYAVCAVTLQALTHALIGLLRNLIVTGLDPERSAIA
                     SQIAIIIIALGIFLPHWLWGLRVARSEVEERESVARRLYLYGMQAALLAPVVNNAFNI
                     AARLLAEVADGGTAQRLHPRLPAGEFILYHLAGAVVLGLVWYYHQRVVAEESSPATEV
                     DGPAAARRLYLLSFSAVGLAMTTIAIVNLLRWILLQIGDAAALSTNGPLSAIARLMVG
                     APLWIVFWVRAERLFSGPEEEERASALRKFYLFSVVFIAALTSVTTASILLASLFRQL
                     LKVADETQGDLRTAIATIVTMAVLWVYHGLVLREDSGRTDESPGQAGVRRLYTYLVAA
                     IGLGAALLGLGGLVSVLILSLERSGTGSALTRQLAWSAAALIAGLPVWGWPWRGAQVR
                     ASTKGVVGDTERSSLARKLFLYFYLLAGTLVVLGSAVFLLSQILGMLLGLAGPGLSEV
                     GHPIGFGLIAVALWLYHAAALRADRQLGIGAITTELEGRRVVVVDAGDGTMGEAVTDE
                     LKKRIPGIDMDFIRLGAASGQPKAKPVVKDQISEADLIIGPWTMTGGTQVAGEGNVKP
                     ELARAIIHSPARKLLIPTRVEGYEWAGVDRWKHKAIIRQVVSAALQILRGEQVKPVRP
                     LGVGAIVGIVVGGLILLQIVLGPLLSLLLFNTR"
     gene            complement(3447..3932)
                     /locus_tag="VI520_03470"
     CDS             complement(3447..3932)
                     /locus_tag="VI520_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36975.1"
                     /translation="MRTTDRVLLGIVLGVVLLAGTAFALVLLKPEPAYLAEDTPEGVV
                     HNYLLALQREEYSRAYGYLSPNLPGYPRTEEQFGQQVEDSYLIQGINEGSLTTATATV
                     RGTQAEVEVRETVFYDGSLFDSGQYVNTFTVELTQVGDGWRIADSERYFYYCWRTFGE
                     C"
     gene            complement(3955..>4198)
                     /locus_tag="VI520_03475"
     CDS             complement(3955..>4198)
                     /locus_tag="VI520_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36976.1"
                     /translation="GVWALVRLVSRSSFWATRISLVSWPFVFGWLTQSWTYLLFGLAL
                     SGVYLTTHRTVSDDHTIIKERWPSLWAFLTGPKRHS"
BASE COUNT          854 a         1363 c         1236 g          745 t
ORIGIN      
        1 tgttcccgct cgccgtcgta cagccgggtc aacaccagcc gatcggcgtt cgggtgcggc
       61 atcacctctc gaatctcgcc aaccacaatt cgccccggat cccaggctat tccccagacc
      121 cgggtctcct gccgagttgc gggggaagct ccctcgaaag gcaatccaac gtggcggatc
      181 tcttccactt ccagcccggc gaaggtcaag cgctgggcga gctccatgat cgagacctcg
      241 atctcaacga actcgcgcaa ccaggaaagg ggaaccttca tgactcgctg cccacctaga
      301 actgcgccag aaagcgaagg tcgttcgccc agaagtagcg gatgtcttca attccatgtt
      361 tcagcatcgt gatccgctcc ggccccatgc cgaaagcgaa accggagaag agctcgggat
      421 catagccccc gttctccaat acagtcgggt gcaccattcc gcttcccagg atttccagcc
      481 agccggttcg cttgcagacc gggcagcccg ccccgccgca cacgaaacac tctatatcca
      541 tctcggcgga gggctcggtg aaggggaagt aggaggcccg aaatcggatt cgtacgttct
      601 ggccgaaggt gcgccgggca aaggcgctga gggtgccctt caagtcggcc agcgtgatgt
      661 ggtggccgac cgccaagccc tcgacctggt tgaactggat ctccgagcgg gccgtgattt
      721 gctcatagcg gaagaccatc cccggcagca ccacccggat cggttccggg gcccgctccc
      781 gcatcacccg gatctggccg ggcgaggtgt gggtgcgcag cagcacctcg gggtcggtgg
      841 tgtagaaggt gtccagcatg tcgcgggccg ggtgatggcg cggcatgttg agcaggccga
      901 agttgtactc gtcggtctcg acctcccgcg agcggtagac ctgaaagccc atctcggccc
      961 agatgtggat gatctctcgc agggtgcggg acgcagggtg gagccggccc cggatcggcg
     1021 gacggccggg cagggtgacg tcgagccgct gctcggcgag cgaccggtcg agttcctccc
     1081 ggcgcagccc ttcttcccgc gccgcgaagg cttgttccaa tccggccttg acctcattcg
     1141 cccgacgacc gaccgctggt cgctgatcct tgggcaactc gcgcagcccg tccagaatct
     1201 gcgccaggag ggccgaacga ccgaggtggt tcaccttcca ggtctgcaaa tcggcggaag
     1261 tacgaatggc ctccagctgc gccagtgcct cgcgttcgag ctgtcctagt cgatctaaca
     1321 tcctgcgttc tccaaaacca aaactcccgc cccacaaggg acgggagcct cgcccgtctg
     1381 ggattatccg cgaatcgcag gtcttgtcaa cgtccagcct gggtggacgg cccgaaccct
     1441 gcgtctaccg cgtattgaaa agcagtaagg agagcagcgg accgagaacg atctgcagca
     1501 ggatcaggcc gccgacaacg ataccaacga ttgcacctac accgagcggc ctgaccggct
     1561 ttacctgttc gccgcgcaga atctggaggg ccgcgctcac tacctgccgg attatggcct
     1621 tgtgcttcca acggtccacg cccgcccact cgtagccctc gactcgggtc ggtatcagca
     1681 gcttccttgc cgggctgtgg atgatcgcac gagcgagctc cggtttgacg tttccttccc
     1741 ctgccacttg cgttccgcca gtcatcgtcc atggaccaat gatcaggtcg gcctcggaga
     1801 tctggtcctt caccaccggt ttggccttag gctgcccact tgccgctcct aggcggatga
     1861 agtccatgtc gattcccggg attcgtttct tcaactcatc agtgacagct tcacccatag
     1921 ttccgtcgcc cgcatcgact acaaccactc gccttccctc cagctcggtg gtaatcgccc
     1981 cgatcccaag ctgtctgtcc gcccgcaggg cggcagcatg gtatagccag agagcaaccg
     2041 cgatgaggcc aaagccgatc gggtggccaa cctcgctgag ccccggaccc gccaagccaa
     2101 gcaacatgcc caggatctgt gagagcagga agacggcgga gccgagaacc acgagcgtac
     2161 cagccagcag gtagaagtat aggaagagct ttcgtgcgag cgagctgcgt tcagtgtcac
     2221 ccacaacccc cttggtagat gccctaacct gcgctccccg ccatggccag ccccagactg
     2281 ggaggccggc gatcagcgca gcggcagacc aggccagctg ccgggtcaga gccgatccgg
     2341 ttccggatcg ctctagcgag aggatcagga cactgaccaa cccgcccaag cccagcaagg
     2401 ctgcacccag accaatcgct gcgacaaggt aggtatatag acgccgcact ccggcctggc
     2461 ctggcgactc gtctgtccgg ccggagtcct ctcgcaggac caacccatgg tagacccaga
     2521 gcacagccat cgttacgata gtggcaatgg ccgtacgcag atccccttga gtctcgtccg
     2581 ccactttgag cagctggcgg aaaagggatg ccaggaggat ggaggccgtc gtgactgaag
     2641 tgagggcggc gatgaacacc accgagaaca gatagaactt gcgaagtgcc gaagcccgct
     2701 cctcttcctc tggcccgctg aacagccgct cggccctcac ccagaagacg atccagaggg
     2761 gtgcgccaac catcagtcgc gcgatggccg agagcggtcc attcgttgat aacgcagcgg
     2821 cgtccccgat ttgtaagagg atccatcgca ggaggttgac gatcgcgatc gtcgtcattg
     2881 ccaacccgac tgcgctgaag ctcaacaagt agagcctgcg cgcggcggcg ggtccatcca
     2941 cttcggtggc cggcgagctt tcttcggcaa ccacccgctg gtggtagtac cagacgagtc
     3001 ccaggacgac cgctccagcc aggtgataga ggatgaattc acccgcgggc agtctgggat
     3061 gcagccgttg agctgtacct ccgtcggcaa cctcggccaa cagccgggcc gcaatgttga
     3121 aggcgttgtt cacaaccggt gctaagagtg ccgcttgcat accgtagaga tagagccgcc
     3181 gcgcgacaga ctcgcgttct tcgacctcgc tacgagccac ccggagcccc cagagccaat
     3241 ggggcaggaa gatccccagg gcgatgatga tgatcgcgat ctgggacgcg atcgcgcttc
     3301 gttcgggatc gagtccagtt acgattaagt tgcgcaacaa gcctatcagc gcgtgggtta
     3361 gggcctgaag cgtgaccgcg catacggcat acacataaag acgccttgga gtggacatgt
     3421 ctgcctccta atccgccact tgcccctcaa cactctccaa acgtacgcca gcagtagtag
     3481 aagtaacgct cggaatccgc aatcctccat ccatctccca cttgagtcaa ttccaccgtg
     3541 aaggtattca catactggcc gctgtcgaac aaacttccgt cataaaagac cgtctcacga
     3601 acctcgactt ccgcttgagt gccgcggaca gtcgcggtcg cagtcgttag gctgccttca
     3661 ttgatccctt gaatcaagta ggagtcctcc acttgttgcc cgaactgttc ttcagtgcgg
     3721 gggtagccag gcaagttagg agagaggtaa ccataggcgc gactgtactc ttcacgttga
     3781 agcgcaagca ggtagttatg aacgactccc tcgggagtgt cctccgccag gtaggccggt
     3841 tccggtttga gcagaacgag cgcgaaggcg gtccccgcca aaagcaccac cccaaggaca
     3901 atcccgagca ggaccctgtc tgtggttctc atccattgcc tcctctggag gccgctagct
     3961 atgccgcttg ggaccggtca ggaaagccca caaactgggc caccgctctt tgatgatcgt
     4021 atgatcgtcg ctgacggtgc gatgggtcgt caggtagacc ccactgagcg ccagaccgaa
     4081 gagcaggtag gtccaggatt gggtcagcca gccaaagacg aacggccagc tcaccaggct
     4141 gatccgggtc gcccagaagg agctgcggct gaccaagcgc accagggccc agaccccg
//
LOCUS       DATDPQ010000147         3464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2930088, whole genome shotgun sequence.
ACCESSION   DATDPQ010000147 DATDPQ010000000
VERSION     DATDPQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2930088"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3464)
                     /locus_tag="VI520_03480"
     CDS             complement(<1..>3464)
                     /locus_tag="VI520_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HLF36977.1"
                     /translation="VIPALKPGDPLPDTFPHLFIIVDEFAEMIMHNPEYKAQFESITR
                     LGRAFGATLILATQRPAGMVTDQMRSNMKFRICLRVETSEDSKELLNRPDAARLPALG
                     GRGYLQVGGGHLTELQAAWAGGDYSDVPPDPKYPTEKVAEALGLPAGSKPGLLIDWLV
                     GALATEANRQSVPKQMKPWPNPLPLLLPLNQPVDATYLKGERADKVETVLINPMVDAW
                     IRNKKEQSLWQAWDWHSPLPLRTDVGLIDNPFQAEQRLLTFDLATDPLAIFGAAGRGK
                     TTLLKSLILSLAASRSPAELNVFALDFGRGGLKAISKLPHCGATIDASQPERVEQLFR
                     MIHGQMKERQERLSQFASLEDYNAQRRDDPENRFPAILVVIDNFAEFNESYEPLLPDL
                     IALIRDGRALGIYYVITAGTPNDLGGKLYNLFSQRVTFTLADASMYADVVGRGALSLA
                     NTPGRGLVNQEGQPLEFQVGMPVIEGEKDPYSVIGERMEKIWLGQGGRRPAAELPRSV
                     TILEMYSMMLGKRVDRIGDIPMAENWKQSMLPENQEWLRAPIGLISSREVRELVFSAK
                     ADGDGVHGMIAGTTGSGKSELLLTLIAAMAIRYDPRIVNFVLVDFKGGAAFEPFKKLP
                     HVVDIATNLQGNAVERIFIALKAELDRRSKLLADGRVGDLVDYRKRVIPKLKKGDPLP
                     DTFPHLFIIVDEFAEMIMQNPEYKAQFESITRLGRAFGATLILATQRPAGMVTDQMRA
                     NMKFRICLRVETPDDSKELLKRPDAATLPALGGRGYIQIGGGPLLELQAAWAGGEYTD
                     IGQDPAYTTAEMLEALNMGPDNQPGLLIDWMVGALAAEAKRQRIPKQLKPWPDPLPPV
                     LALDTPIDASYLKPATADGKLVINPVVAGWMENTKAKPLWEPLDWSKPPALQATLGLL
                     DNPYQAEQRLLNIDLAGDPLVVFGAAGRGKTTFLKSLILSLAAEFSPADLHIFALDFG
                     RGGLKALKVLPHLGGIVDANEEERVERLMRMLRTILDDRQHKLQAYDSLADYNEKHLK
                     DSLPRVLVVIDNVSEFKETYEQHLLDLIAMVRDGRAFGVHFAITAPLIADVHSKLFNI
                     LGQRITFTQADPSDYSTIVGRGWARFNDEPGRGLAVEMLDGRPQPLEFQTGVPAGEPD
                     GEAYRELA"
BASE COUNT          619 a         1117 c         1053 g          675 t
ORIGIN      
        1 tgggcgagct ctcgataggc ctccccatcc ggctcgccag ccgggacgcc ggtttgaaac
       61 tccaacggtt gcggccggcc gtcgagcatc tcgaccgcca acccccgccc cggttcgtcg
      121 ttgaagcggg cccaaccgcg cccgacgatc gtggagtaat cgctcgggtc ggcctgagtg
      181 aaggtgatcc gctgtcccag gatattgaac agtttactgt gcacgtccgc aatcagcggg
      241 gcggtgatcg cgaagtggac gccgaaggcg cggccgtccc ggaccatcgc gatcagatcc
      301 agcaggtgct gttcgtaggt ctccttgaac tcggaaacat tgtcgatcac aactagcacc
      361 ctgggcagcg agtctttcaa atgtttctcg ttgtaatcgg ccagcgagtc ataggcctga
      421 agtttgtgct ggcgatcatc gagaatcgtg cgcagcattc tcatgagccg ctcgactcgt
      481 tcctcttcat tggcgtcgac aatcccgccg aggtgcggaa ggaccttgag tgccttgagc
      541 ccgccgcggc cgaaatcgag tgcgaaaatg tgcagatcgg ccggcgagaa ctcggcggcc
      601 aacgaaagga tcagcgattt caagaaagtg gtcttgcctc gaccggcggc cccgaatacc
      661 accagcggat cgccggccag atcgatgttg agcaatcgtt gttcggcctg atacgggttg
      721 tccaacaatc ccagggtcgc ctggagggcg ggcggcttgc tccaatcgag cggctcccag
      781 agcggtttcg ctttcgtatt ttccatccac ccggccacga ccgggttgat gacgagcttg
      841 ccgtcggcgg tggccggctt gaggtagctg gcatcgatcg gagtgtcgag ggccaagacc
      901 ggcggcagtg ggtccggcca gggcttgagc tgcttgggga tcctttgccg cttcgcttca
      961 gccgccagcg ccccaaccat ccaatcgatc agcagccccg gctggttgtc gggccccatg
     1021 ttcagggctt cgagcatctc agcggttgtg taggccgggt cttgcccgat gtccgtgtat
     1081 tcacccccgg cccaggcggc ctgcaattcg agcaacggtc cgcccccgat ctggatataa
     1141 cctcggcctc ccagcgccgg cagggtcgcg gcgtccggcc gcttgagcag ttccttgctg
     1201 tcatccggcg tctctacccg gaggcaaatc ctgaacttca tgttggcccg catctggtcg
     1261 gtcaccatcc cggccggacg ttgggtggcc aggatcaggg tcgcgccgaa agctcggcct
     1321 agccgggtga tgctctcgaa ctgggccttg tactccggat tctgcatgat catctcggca
     1381 aactcgtcca cgatgatgaa cagatgcgga aaggtgtctg gcaaggggtc gcccttcttc
     1441 agtttcggaa tcacccgctt gcggtaatcc accaggtcgc caacccgccc atcggccagc
     1501 agcttggatc gccggtcgag ctcggccttg agggcgatga agatccgctc gaccgcgttg
     1561 ccctgcaggt tggtagcgat gtccacaacg tggggcagct tcttgaacgg ttcgaaggcc
     1621 gccccgccct tgaagtcgac caacacgaaa ttgacgatcc gcgggtcata gcggatggcc
     1681 atggccgcga tcaacgtgag cagcaactct gacttcccgg agccggtcgt tccggcaatc
     1741 atcccgtgaa ccccatcccc atccgccttg gccgagaaga ccagctcccg gacctcccga
     1801 ctgctgatca acccgatcgg agcccgcagc cactcctggt tctctggcag catgctctgc
     1861 ttccagttct cggccatcgg aatgtcacca attcgatcga ctcgcttgcc cagcatcatc
     1921 gagtacatct cgagaatggt caccgaccga ggcagctcgg ctgccggccg ccggccaccc
     1981 tgtccgagcc agatcttctc catccgctcg ccgatgaccg agtagggatc tttctcgccc
     2041 tcgatcaccg gcatcccgac ctggaactcg agcggctgcc cctcctggtt gaccagcccc
     2101 cggcccggag tgtttgccag gctcagcgca ccgcgcccca cgacgtcggc atacatcgag
     2161 gcatccgcca aggtgaaggt cactcgctgc gagaacaggt tgtacagctt cccacccagg
     2221 tcattgggag tcccggccgt aatgacgtag tagatgccga gcgcccgacc atcccgaatc
     2281 aacgcgatca agtctggcag caaaggctcg tagctctcat tgaactctgc gaaattgtcg
     2341 atgaccacca ggatggcggg gaagcggttc tcgggatcgt cacgccgctg ggcgttgtag
     2401 tcttccagcg aggcgaactg actcagccgc tcctgacgct ccttcatctg gccatggatc
     2461 atgcggaaaa gctgctcgac ccgctccggc tgggaggcat cgatcgtggc cccgcaatgg
     2521 ggcagcttcg agatcgcctt cagcccgccc cgtccgaaat ccaaggcgaa cacgttgagc
     2581 tcggccgggg atcggctggc tgccagcgag agaatcagcg atttgagcaa ggtggtcttg
     2641 ccccgccccg cggctccaaa aatggccagt gggtcggtcg ccagatcgaa tgtgagcagg
     2701 cgttgttcgg cttgaaatgg attgtcaatc aggccgacgt ccgttcgcaa cggcaggggt
     2761 gagtgccaat cccaggcctg ccacaaggat tgctccttct tgttccggat ccaggcatcg
     2821 accatcgggt tgatcaggac cgtctcaacc ttgtcggccc gctcgccctt caggtaggtc
     2881 gcatcgaccg gctggttgag tggcagcagg agcggcagtg gattgggcca gggtttcatc
     2941 tgcttgggaa ccgactgccg gttggcctct gtcgccaagg cacctaccaa ccaatcgatc
     3001 agcagcccgg gcttgctgcc cgccggcagc ccaagcgcct cggccacttt ctcggtcggg
     3061 tacttcgggt cgggcggtac atcggaatag tcgccgccgg cccaggcggc ctgcagctcg
     3121 gtgaggtgcc ccccacccac ctgcaaatag cctcgccctc cgagcgccgg caaccgggcg
     3181 gcatccgggc gattgagcag ttccttgctg tcctcggagg tttccactct caagcagatg
     3241 cggaacttca tattcgagcg catctggtca gtcaccatcc cggccgggcg ttgggtggcc
     3301 aggatcaggg tcgcgccgaa ggcccggccg aggcgggtga tgctctcgaa ctgagctttg
     3361 tactccgggt tgtgcatgat catctctgcg aattcgtcca cgatgatgaa cagatgcggg
     3421 aaggtatccg gcagcggatc ccccggcttc aaagcgggaa tgac
//
LOCUS       DATDPQ010000148         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1112154, whole genome shotgun sequence.
ACCESSION   DATDPQ010000148 DATDPQ010000000
VERSION     DATDPQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112154"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VI520_03485"
     CDS             complement(<1..452)
                     /locus_tag="VI520_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36978.1"
                     /translation="MDTTMKQAYRVARELLRDRVRKSSPETLEILEQVRQADVIVVEG
                     GYDRIQDVLEIAGMPFTLVSPERFEKSNLRPDQIVFVNCPGNLTPKGLRNLEVFVREG
                     GFLFTTDWALRNVLEHAFPGFVEFNDRPTGDEVVRIELLDREDPMLKTV"
     gene            679..1797
                     /locus_tag="VI520_03490"
     CDS             679..1797
                     /locus_tag="VI520_03490"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HLF36979.1"
                     /translation="MIRKLRIVALLAGWAAIAACTGAAPSTPTSTPTPSASPTATHTP
                     PPTATATPTATATQTPKPTSFLNEAPTLTPDGSAGSSPSSEPITLRVQLQAGETYRIQ
                     LLTRQQIVQNLEGQQFETVQTLGIQYSYGVTEVDSEGNTWMDLLYERVILEQDTVLGE
                     VKYDSADPPAEIPPEAQGLSALVGLGFSLRMSPRGEVLETRGEDELVEQMIAALQISD
                     PAAREQMETTLRQQYGGEGLENQLRDTVVEFPEAPLRVGDSWSITTETFALTTLTVEA
                     TYTLRGFDEQTARIDVRSSFTTADSMEMLDFGLFQLGYVLAGVQEGTMLVNLQTGLSD
                     IQLHQDLSGEMTIAAEDEQLTVPLTAQGVFQVRVVEQD"
     gene            complement(1864..2073)
                     /locus_tag="VI520_03495"
     CDS             complement(1864..2073)
                     /locus_tag="VI520_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLF36980.1"
                     /translation="MTDRVQGSVKWFNEKKGYGFIEREDGDDVFVHFSAISGDGFRTL
                     EEGQRVEFSIQQGPKGLQAADVIKI"
     gene            2349..3686
                     /locus_tag="VI520_03500"
     CDS             2349..3686
                     /locus_tag="VI520_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF36981.1"
                     /translation="MGRIPYNVGLVAGLRMDRRFAILFTIVFVDLIGFGIVIPVLLLH
                     AQESFGATDLQATMLLTSYSAGLVVAGPVMGRLSDAFGRRPVLILSQIGTFIGFLVLG
                     FANSLFLLYLGRIIDGISGGNITTATAYINDITTEKNRARGFGLISAAFGAGFIVGPA
                     LGGLVVNLAAGIDSLAAYSQNAPFFVAAFFSLLSILGTTFLLPETLAPDQRHPLGQPR
                     PSRLGEGGLMDVLRIANVRIIILFSFIAFLGFALLQSSFPLFARRSLFPALPLLTVER
                     NIGFLLTWVGVVSVTTQSFFVGPLVNRYGERRLVSASAFARILAFLGTALARNSITAA
                     ASFIPLAAANAVNQPSLTSIISRFSPPHMRGRVLGAFQSSNSLTLVIGPIVAGLLLQM
                     RIDGVSPETEAALPLFVAAGLMAIAFLLSFRILRMDLPTQEAGSAGSTAAPAP"
BASE COUNT          708 a         1248 c         1183 g          754 t
ORIGIN      
        1 accgtcttca gcatcggatc ttcccgatcc agcagttcaa tccggaccac ctcgtcgccg
       61 gtcgggcggt cgttgaactc gacgaaccct ggaaaggcgt gctcgagcac gttgcgcagg
      121 gcccagtcgg ttgtaaaaag aaagcctcct tcccgcacga aaacttccag gttgcggagc
      181 cccttcggcg tcaggttgcc cggacagttg acgaaaacga tctggtccgg gcgcagattg
      241 gatttctcga accgctcggg gctgaccaaa gtaaagggca tgccggcaat ctccagcacg
      301 tcctgaatgc gatcgtagcc accttcgacg acgatcacgt ccgcctggcg gacctgctcg
      361 agaatctcga gcgtttcagg ggatgacttg cggactcgat cccgcagcaa ctcccgggca
      421 acccgatagg cctgcttcat tgtcgtgtcc atgcccttcg catcctttga gtgctcggcg
      481 gccccaaggc actctaaggg ggatgatacc gcaaatgggg aagaaagtaa atacttactt
      541 actggctagt tggcgatcgg cacggtggac tcactgggca gccggcataa ctcgtggctc
      601 cggcaaggcg agtcacggtt tcctttctat ttggcgcaat gttcgcgata atgccgcggg
      661 tcgggggaaa acaatcccat gattcggaaa ctcaggatcg tcgcgctgct cgcggggtgg
      721 gcagcgattg ccgcctgtac aggcgcagct ccctcgaccc caacttcaac cccaactccg
      781 agcgccagcc cgacggcgac ccacacaccc ccgccgacgg cgaccgccac gccgaccgcg
      841 acggcaaccc agaccccgaa gccaacctcg ttcttgaacg aggccccgac cctgacgccg
      901 gacggttcag ccggctcgtc gccaagcagc gaaccgatta cgctgcgggt ccagctccag
      961 gccggtgaga cctatcgcat ccaactgctc actcgccagc agatcgtcca gaatctggaa
     1021 ggccagcagt ttgagaccgt tcagaccctc ggcatccaat acagctatgg ggtgaccgag
     1081 gtcgattcgg aggggaacac ctggatggat ctgctctacg agcgggtgat cctggagcag
     1141 gacaccgtgt tgggagaggt gaagtacgac tcggccgatc cgccggccga gattccgccg
     1201 gaggcacagg gcctcagcgc gctggtcggc ttgggtttct cgctgcggat gtcgccgcga
     1261 ggggaggtgc tcgagactcg cggcgaggat gagctggtcg agcagatgat cgcggccttg
     1321 caaatcagcg atcccgccgc ccgggagcag atggagacca ccctccgcca gcaatacggc
     1381 ggagaggggt tggagaacca gctgcgggac acggtggtcg aattccccga ggccccattg
     1441 cgagtcggcg acagctggtc gattactacc gagaccttcg cactgacgac tctgacggtc
     1501 gaagccacct ataccctgcg cgggtttgac gagcagacgg cccggatcga cgtgcgttcc
     1561 agcttcacga ccgcggactc catggagatg ctggacttcg ggttgttcca gctcggctac
     1621 gtgctcgccg gcgttcagga agggacgatg ctggtcaacc tccaaaccgg tctatcggac
     1681 attcaactgc accaagatct gagcggcgaa atgacgatcg cggcggaaga cgagcagttg
     1741 accgtcccgt tgacggctca gggagtgttt caggtccggg ttgtggagca ggattaactc
     1801 ggttgaactg aaagaaaaag cccccgtgcg cgcgggggct ttcgtttgca ggttcaggct
     1861 ggcttagatc ttgatgacat cggccgcctg cagacccttg gggccctgct ggatggagaa
     1921 ctccacccgc tggccttcct caagggtgcg gaagccgtcg cctgagatcg ccgagaagtg
     1981 tacgaacaca tcgtcgccat cttcgcgctc gatgaatccg taccccttct tctcattgaa
     2041 ccacttcact gaaccctgta ctcgatccgt catgcgtttc cttgcctcct tacggcagta
     2101 atcaatatag gtgccggtct tgttctcgga ggccggggtg ggtgcttaac cacatcggcc
     2161 tgagctgcgc acgctcaggc caagatctca cgacttcctg acagaacttc catgcaacct
     2221 actgttagaa atgttaccac ggctttcgtc gggcgcaaga ggcccgccgg gcgaacgagg
     2281 ctgtcgaccg agagggccgc cggcgcgagg cggctgcgcg ggggagcggc gcaggccggc
     2341 gatccggcgt gggtcgcatc ccttataatg tcggacttgt ggccgggttg aggatggacc
     2401 gccgcttcgc gattctgttc acgatcgttt tcgtcgacct gatcggattc gggatcgtaa
     2461 tcccggtgct gctgctgcac gcccaagaat ctttcggggc caccgatctg caagcgacca
     2521 tgctgctgac ttcgtactcg gccgggctgg tggtagccgg gccggtgatg gggcggctct
     2581 cggatgcttt tggccggcgg ccggtgctga tcctgagcca gatcgggacc ttcatcggat
     2641 tcctggtgct gggtttcgcc aactcgctgt tcctgctgta cctcgggcgg attatcgacg
     2701 ggatctccgg cggcaacatc acgactgcca ccgcctacat caacgacatc acgaccgaga
     2761 agaaccgggc ccgcggcttt ggactgatca gtgccgcgtt cggggccgga ttcattgttg
     2821 ggccggcgct ggggggcctg gtggtcaacc tggccgccgg catcgactcg ctggccgcct
     2881 acagccaaaa cgcaccattc ttcgtggcgg ccttcttttc gctgctctcg atcctgggca
     2941 ccaccttcct gctccctgag accttggcgc cagaccaacg acacccgctc gggcagcctc
     3001 gaccgagccg gctcggcgaa ggggggctca tggatgttct gcgcatagcc aacgttcgaa
     3061 tcatcattct gttctcgttt atcgccttcc tgggctttgc gctgctgcag tcgagcttcc
     3121 cgctcttcgc ccgccgcagc ctatttccgg ctctcccact gctaacggtg gagcgtaaca
     3181 tcgggttctt gctgacctgg gtgggagtgg tcagcgtcac cacccagagt ttctttgtcg
     3241 gtccgctggt caaccgctat ggagagcggc ggctggtctc cgcctccgcc ttcgcccgga
     3301 ttctggcgtt cttgggcacc gcgctggccc gcaactcgat aaccgcggcg gcatccttta
     3361 tccctttggc cgcggccaac gcggtcaacc aaccctcgtt gacctcgatc atcagccgct
     3421 tttctccgcc gcacatgcgc ggccgggtgc tcggggcatt tcaatcctcc aacagcctga
     3481 ccttggtgat cggcccgatc gtggctggct tgctgctcca aatgcgcatc gacggagtgt
     3541 caccggagac cgaggcggcg ctaccgcttt tcgtcgcggc cgggttgatg gccatcgcct
     3601 tcctgctcag cttccgcatc ttgagaatgg acttgcccac ccaggaggcc gggtccgccg
     3661 gatccacggc cgctccggca ccttagccgg ccaagaagga agctccccga aggccgaccc
     3721 gccgcgcggg aagccgcacc gatcctatgc ctaccaatct gccaccccaa tacttcgagg
     3781 ccgagaagcg ctacaaagaa gccaaagacc cgaccgagaa ggtcgagcgc ctcgaggagc
     3841 tgatcgggac gatccccaag cacaagggga ccgatcacct gcgcgccgat ctg
//
LOCUS       DATDPQ010000149         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3209878, whole genome shotgun sequence.
ACCESSION   DATDPQ010000149 DATDPQ010000000
VERSION     DATDPQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3209878"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VI520_03505"
     CDS             <1..666
                     /locus_tag="VI520_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLF36982.1"
                     /translation="VMAQHGYKSDRDELLEEAEMLYRHGFGVLFTTVRAHDASDGELI
                     TFGALEMKDLEAWYQFLLGRPEIDVQRIGIIGNSMGGFLAIQYTALNPRIRAVVAHSP
                     FSSMDDTVATSVEAFTGLPAFPFAPLIVFWAEQMAGIDSAEIDAKQSIGTISPRPVFI
                     LMGGQDQSVLVASGELLYEAAGEPKELWFEPDLGHVAFDTELPEEFEARVVGFLETYL
                     GGG"
     gene            730..1257
                     /locus_tag="VI520_03510"
     CDS             730..1257
                     /locus_tag="VI520_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HLF36983.1"
                     /translation="MINFLQVTAGLALLILGRRLFWLALAALGFVAGMTYSSEFLPGQ
                     SETAALLIGLAAGVIGALLAVFMQKIAIGAAGFLAGGYLVLGLFRLIGAEPSAFGFIV
                     GGIVGLILIYPLFDWGLIILSSLAGALILVQTLDAARAVSAIVFAVALVFGLIIQSAL
                     HRRRPKRPAEPPQAG"
     gene            complement(1323..1886)
                     /locus_tag="VI520_03515"
     CDS             complement(1323..1886)
                     /locus_tag="VI520_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36984.1"
                     /translation="MTDPEFQLRVLESAVGELESYLLSPEVFWRVSGAADLPNLTLGG
                     LELARRALVATIGKLDPAARPRAHIVIEQIDEFRRQSRFAWERKAASELKVRLNQWQA
                     YLQDLAEQPGESERYPQEVRARAMATYLLEEAAHQPQADETLKRLEALDARLRVSFAS
                     GIFIWDPSLAEAYPAGTHWFLYGRPKT"
     gene            complement(1883..2257)
                     /locus_tag="VI520_03520"
     CDS             complement(1883..2257)
                     /locus_tag="VI520_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36985.1"
                     /translation="MAEPLRILVVDDERDTLDLIDLTLSSAGYTISLANSGAESLEKI
                     RQDSFDVILMDIMMPDMTGFDVLRTLNEERKDLPPVIFLTAKSLPEDRQMGLQLGAKD
                     FLIKPVTRGSLLDSIKKNTQTN"
     gene            complement(2296..>2538)
                     /locus_tag="VI520_03525"
     CDS             complement(2296..>2538)
                     /locus_tag="VI520_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HLF36986.1"
                     /translation="SNLGMYQVDHFIAIINPPEAAILAVGSVREEPVVQAGAIVPGTR
                     LQVTLSADHRVTDGAEAARWLQVFRSYTEAPVHLLV"
BASE COUNT          429 a          759 c          827 g          523 t
ORIGIN      
        1 gttatggccc agcacggtta caagtccgac cgggatgagc tcctggagga ggccgaaatg
       61 ctgtaccgcc acggcttcgg cgtgctgttc acgacggttc gggcccacga cgcaagcgat
      121 ggagagctga ttaccttcgg cgcccttgag atgaaggatc tggaagcgtg gtaccagttc
      181 ttgctcggcc gcccggagat tgatgtgcag cggattggga tcatagggaa ctcgatggga
      241 ggcttcctgg cgatccagta caccgcgctg aatccaagaa ttcgagccgt ggtggcgcac
      301 agcccgttct cgtcgatgga cgatacggtg gccaccagcg tcgaggcgtt caccggcttg
      361 cccgcctttc cgttcgctcc gctgatcgtc ttctgggcgg aacagatggc cgggattgat
      421 agcgccgaaa tcgacgccaa acagtcgatc ggcacgatca gcccccggcc ggtcttcatc
      481 ctgatggggg gccaggatca gtcggtcttg gtcgcgagcg gagagctgct ctacgaagcc
      541 gccggtgagc cgaaagagtt atggttcgag ccggatctgg gtcacgtggc cttcgacacc
      601 gagttgccgg aggagttcga ggcccgagtc gtgggtttcc tcgagaccta tctcggtggt
      661 ggctgagagg caggccccgc gggccaagtc caccatgggc tatcaagttc ttcggtcgaa
      721 ggagcaacga tgatcaactt tcttcaagta actgccggat tggcactgct gatcctgggc
      781 cggcggctgt tttggttggc gctggccgcc cttgggttcg tggctgggat gacctacagc
      841 agcgagttcc tgcccgggca atccgagacg gcggcgctct tgattgggct ggcggccggg
      901 gtgatcgggg cgctgctggc ggtcttcatg caaaagattg caatcggggc ggccggattc
      961 ttggcgggtg gttatctggt acttggcctg ttccggctga tcggcgccga gccttccgcc
     1021 ttcggcttca tcgtcggcgg gatcgtcgga ttgatcctga tctacccgct cttcgactgg
     1081 gggttgatca tcttgtcttc gctggcgggg gccctaattc tggttcagac cctagatgcc
     1141 gcccgggctg tgtcggccat cgtgtttgcg gtagccctcg ttttcggatt gattatccaa
     1201 tctgcgctcc accgccgccg tccgaagcgg cccgcagagc cccctcaagc gggctgactg
     1261 cgcatagcca ggccgagcct tctcgcaaca tggctcccgg ctccggcccc tgagccgagg
     1321 cgtcaggtct taggtcgtcc gtagagaaac caatgggtcc ccgcaggata agcctccgcc
     1381 agggaagggt cccaaatgaa aatgcccgag gcgaacgaga cgcgcagccg ggcgtcgagc
     1441 gcctctagcc ttttgagcgt ttcatcggcc tgcggctggt gggccgcttc ctcaagcagg
     1501 taggtcgcca tggctcgggc gcgaacctcc tgcgggtaac gttccgattc acctggctgc
     1561 tcagccagat cctggagata ggcctgccac tgattcagcc gcaccttcaa ttcgctggcc
     1621 gccttccgct cccaggcgaa ccgcgactgg cggcgaaact cgtcaatttg ctcaatcact
     1681 atgtgggctc gcggtcgggc tgccgggtcc aatttgccga tggtggcgac cagggcgcgg
     1741 cgggcaagct ctaggccgcc gagggtcagg ttgggcagat cggccgcgcc tgaaactctc
     1801 cagaacactt ccggcgagag cagatagctc tccaactcgc cgaccgcgct ttcgaggacc
     1861 ctgagctgga actcggggtc cgtcaattgg tttgggtgtt tttcttgatg gagtcgagca
     1921 acgagccgcg agtgaccggc ttgatgagga agtctttggc cccgagctgc agccccattt
     1981 gccgatcctc gggcagcgac ttggcggtca gaaagatcac cgggggcagg tccttccgtt
     2041 cttcgttgag ggtccgcagc acatcgaagc cggtcatgtc cggcatcatg atgtccatca
     2101 ggatcacgtc gaaactgtcc tggcggatct tttcgagcga ttcggctcca ctgttggcga
     2161 ggctgattgt gtacccggcc gagctgaggg tcagatcgat caggtcgagc gtgtcacgct
     2221 cgtcgtccac cacgaggatt cggagaggtt ctgccaaggt gtcgatcccg gcccggaggc
     2281 ggcggaattg tacactcaga ccaataagtg caccggggct tcggtatagc tcctaaatac
     2341 ctgcagccag cgggcggcct cagccccgtc ggtcacccgg tgatcggcgg agagcgtgac
     2401 ctgcagccga gtccccggga cgatcgcccc ggcctgcaca accggttcct cccggacgct
     2461 tcctacggcc aggatcgcgg cctccggcgg attgatgatg gctatgaaat gatcgacctg
     2521 gtacatcccg agattgga
//
LOCUS       DATDPQ010000150         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_322644, whole genome shotgun sequence.
ACCESSION   DATDPQ010000150 DATDPQ010000000
VERSION     DATDPQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322644"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..655
                     /locus_tag="VI520_03530"
     CDS             299..655
                     /locus_tag="VI520_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF36987.1"
                     /translation="MDDNQAMVEALSMVVSARGHVALSAHSGEEALTVVANEVPDVVL
                     LDLKMPGIDGFETLGRLRKMPQAEGLPVLIITAQDEADLESRVAAAGGNGLLKKPVDV
                     NLLAAQIALHTAKAEV"
     gene            836..>2197
                     /locus_tag="VI520_03535"
     CDS             836..>2197
                     /locus_tag="VI520_03535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HLF36988.1"
                     /translation="MEIGTVRQVDIDEQMRGAYLDYAMSVIVSRALPDAFDGLKPVQR
                     RILYAMREMGIRANSPYRKSARIVGEVMGKFHPHGDAPIYEAMARMAQDFSMRYPLID
                     GQGNFGSVDGDPPAAMRYTEARLGRMAEELLIDIEQDTVGFSDNFDGSLEEPNVLPAR
                     LPNLLLNGATGIAVGMATNIPPHNLGELAEAIVYLVDHYDQVDDVGVDDLMKFVQGPD
                     FPTGGMIIGAEGILQAYSTGKGRIVVRGIANIEETGHSRNRIVITEIPFQVNKTALIE
                     RIAELVRDGKLDGISDLRDESDRRGLSVVLELKRTAQPKAVLNQLYKYTQLQTTFGVQ
                     QLALVNGEPRLLSLKRALLAYLEHRRTVITRRSKFELEKARARAHVLDGLLIALANLD
                     EVIDTIRKSPDADAAMQRLIQRFKLSEIQAQAILDMQLRRLAALERKNIETEAKEIKD
                     RIAA"
BASE COUNT          456 a          641 c          710 g          390 t
ORIGIN      
        1 catgcggtga tcgatgccca ccacggccag atccgggtcg agagcgaagt ggggcaagga
       61 acgaccttcg aaatcgcagt gccgagcggc tcttgattgg ggtagcccgg cccaacaatt
      121 gcaacctggc tcgggcgagc cgcaggttgc cgcgagggat gggagccgcg gtctgatcgg
      181 atagctctca aggacgagta gtaatgcagt caaacttgga gatagaatag gtggggacgg
      241 cgggcaacca cggggttctc cggcgaaggg agtccaggcc ctggccagaa ttctgattgt
      301 ggatgacaac caggctatgg ttgaggcgct gagcatggtg gtcagtgccc gaggccacgt
      361 cgcgctctcg gctcacagtg gggaagaggc gctgaccgtg gtcgccaacg aggtaccgga
      421 cgttgtgctc ttggatttga aaatgcccgg gattgacggg tttgagaccc tcggccgcct
      481 gcgcaagatg ccgcaagccg aggggctgcc cgtgctgatc atcaccgccc aggatgaggc
      541 cgatctcgag agtcgggtgg ccgccgccgg cgggaatggc ttgctgaaga agccagtgga
      601 cgtcaacctg ctggccgccc agattgccct gcataccgcc aaggccgagg tctagccctc
      661 gaccaggtag aatcccagac ccagccaaga tcgaacgaaa tggaccgcac cgtgggtgcg
      721 gttctcgcgt tagatcctgt tcgcatcggc gaatcccaac gactataatg ggtgcagggg
      781 tcggcctgct gctgggcggg cccctttgta tttgacataa ggagtagatt ttcagatgga
      841 gatcgggacc gtacgccagg ttgatatcga cgagcagatg cggggcgcct acctcgatta
      901 cgccatgagc gtaatcgtat cccgggcatt gccggatgcc tttgatggac tgaagccggt
      961 ccaacggcgc atcctttacg ccatgcgaga gatgggaatt cgggccaatt cgccgtaccg
     1021 aaaatcggcc aggatcgtcg gtgaagtcat ggggaagttc catccccacg gcgacgcgcc
     1081 gatctacgag gccatggccc gcatggctca ggacttctcc atgcgttatc cgctgattga
     1141 tggccagggg aacttcggct cggtggacgg cgatccgccc gctgcgatgc gatataccga
     1201 ggcccgcttg ggccggatgg ccgaagagct gctgatcgat atcgagcagg acacggtcgg
     1261 tttctccgat aacttcgatg gttcgctgga ggaacccaac gtcctgcccg ctcggctgcc
     1321 gaacctgctg ctcaacggtg ccaccggcat cgcagtcggc atggcgacca acatcccacc
     1381 ccataacctc ggcgaactgg cggaggccat cgtttacctg gtggatcact acgaccaggt
     1441 ggacgatgtc ggggtggatg acctgatgaa gtttgtgcag gggcccgatt tcccgaccgg
     1501 agggatgatc atcggcgcgg agggtatcct gcaggcctac agcaccggca aagggcgaat
     1561 cgtggttcgc ggaatcgcca atatcgaaga aaccgggcac agccgcaacc ggatcgtgat
     1621 taccgaaatt cccttccagg tcaacaagac ggccttgatt gagcggatcg ccgagctggt
     1681 ccgagacgga aaactggacg ggatctcaga cctgcgcgac gagtccgatc ggcgcggcct
     1741 gagtgtcgtg ctggagctca agcggaccgc ccagccaaag gcggtcttga accagctcta
     1801 caaatacacc caattgcaga ccaccttcgg ggtgcagcag ctggcgttgg tcaacggcga
     1861 gccgcggctg ctctcgctca agcgagcgtt gctggcttat ctggaacacc gccgcaccgt
     1921 gataacccgc cgctcgaaat tcgagctcga gaaggcccgg gcccgggccc acgtgctgga
     1981 tgggctgctg atcgccctcg ccaacttgga cgaggtgatc gataccattc gcaagtcccc
     2041 cgatgccgac gccgctatgc agcgcctgat ccagcgcttc aaactcagcg agatccaggc
     2101 ccaggcgatc cttgatatgc agctgcgccg gttggccgcc ctggaacgca agaacatcga
     2161 gaccgaggcc aaggagatca aggacagaat tgcagct
//
LOCUS       DATDPQ010000151         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2161115, whole genome shotgun sequence.
ACCESSION   DATDPQ010000151 DATDPQ010000000
VERSION     DATDPQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161115"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VI520_03540"
     CDS             <1..1833
                     /locus_tag="VI520_03540"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HLF36989.1"
                     /translation="LIGSRVAVWIVAQVHLMFAAFMLGVPMFALVVEYVGFRSREQRY
                     DDLAREFTKLILVAASTTAAFGAVLTFLLFTLYPQVMSRLADVFLPTMVLYPLLIFGE
                     VFALYVYWYSWDRLNSPRGKRWHLLIGLVLNLMGTALMFVANAWLTFSISPESGGQPL
                     YDQAGQFTGTLWQAVNNATWWPINIHRVIANIAFGGAVVGLYAAIRFLNSHSAEERAH
                     YDWMGYVGNFIAVIGLIPLPFAGYYLGREVYGFSQQMGVTMMGGFLSWLWILQAMLIA
                     VIFLSANYYLWIGMGRIQGGERYRGRIKWLLAILTLGVLVWATPHNPVVTPEEQALIG
                     GAFHPLLGVLGVMSAKNTAVNLMILTTFVSFLFYRRAGKGEPLPFARQGNPAKIVLIV
                     VLLITVLLSLAPALYVLRDGLGETSRLLRVGLSALHGLLLIVGVILTFRDRGRLGQWI
                     ILGSAASVVIFFGVYGYFVEATIRIGFSVYQVLAVLTALISSTAIDVFLYQGAPSLGT
                     IEWGKIPARAQYVLILLAVGFTWLMGLMGYARNAIRQHWHIYEVLQDTSVDAFSPTLG
                     FAARVISVCVLVFLGLMGFIFWLGNLGEKKAPAALRPEPLPATD"
     gene            1834..2616
                     /locus_tag="VI520_03545"
     CDS             1834..2616
                     /locus_tag="VI520_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF36990.1"
                     /translation="MGNSLKVAGFIVLVVVLIAGFASLIPQLESPAPEELVISGELSG
                     TALADVGEEVFNSAAAGCLACHGLGKPGLRAPDLAGIGAAAGDRVVGLSPEQYLRQAL
                     TEPCSYVVSGYDCIMPQSLQQTLGEAKLTALVAFLQSLGGEVTVRLSAEAAQAAGGEA
                     GGGTAGVTAAEIFTNAGCVACHTLPIVGAAGQVGPDLSEVGARLTPDEVRRSILFPDE
                     QLAENCPTGPCLAGLMPKAFGESLSGLQLETLVTFLSEQGVP"
     gene            2613..3419
                     /locus_tag="VI520_03550"
     CDS             2613..3419
                     /locus_tag="VI520_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF36991.1"
                     /translation="MSLVRRVVGHKLGQFALVLIGAYLLFRLGVPWLSRTLTGQSAPV
                     PAHLLWTIYMPIVLLVMLLFVSASEDSWQEFQRPLRELIIEQERAPLIWTRRVVGIGL
                     PLLAGLFAYLQVRPEVSAPPELRSVHPAPASRITLDGQAIDLRTAVNPFRGASGAADP
                     TALAEGKAIYGQYCVYCHGDNLAGNGLFAGRLRPRPADFTDPGTIAQLQESYLFWRIA
                     TGGPGLPPEAKGWNSAMPAWEGTLAADDIWKVILYLYDATGQQPRAVSES"
     gene            3420..>4026
                     /locus_tag="VI520_03555"
     CDS             3420..>4026
                     /locus_tag="VI520_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF36992.1"
                     /translation="MSTRARWVAALILAAVGLAAARSPHQAKQSPAQQPAAGEAVYQQ
                     RCEICHGAAGDGNGAAAANMDPRPRDFRRGWYKIRSSANGQLPTDADLLAIITNGMPG
                     TTMPAWGEILSPDELQAVAGYLKGFSRRFERETPEPVSVVAAPGSSEERIGGGALLFS
                     GAQAECAKCHGIAGRGDGPSAGELVDDFGQPIVPADLTTPWS"
BASE COUNT          648 a         1218 c         1319 g          841 t
ORIGIN      
        1 ctgatcggca gtcgagtcgc ggtctggatc gtggcccagg tccatctgat gttcgcggca
       61 ttcatgctcg gagtgccgat gttcgcgctg gtcgttgagt atgttggatt ccgtagccga
      121 gagcagcgct atgacgatct cgcccgcgaa ttcaccaagc tgatcttggt agcggcctcg
      181 acgaccgccg cgttcggtgc cgtgcttacc tttctgctct ttaccctcta cccccaggtc
      241 atgagccggc tcgccgatgt cttcctgccg accatggtct tgtacccgct gctcatcttt
      301 ggggaggtct tcgctctcta cgtgtactgg tattcctggg atcggcttaa cagtccccgg
      361 ggaaagcgct ggcacttgct catcggccta gtgcttaacc tgatgggcac ggcgctgatg
      421 tttgtcgcca atgcctggct caccttttcg atcagccccg aatcaggagg ccagccgctc
      481 tacgaccagg ccggccagtt caccggcacg ctctggcagg ccgtcaacaa cgccacctgg
      541 tggccgatca acatccatcg ggtaatcgcc aacatcgcct tcggaggcgc ggtggttgga
      601 ctgtatgcgg cgatacgatt cctgaactcg cactcggcgg aagagcgggc tcattacgat
      661 tggatgggtt acgttggcaa cttcatcgca gtgatcggat tgatcccgct gccgttcgcc
      721 ggctactatc tcgggcgcga agtctatggg tttagccagc agatgggcgt caccatgatg
      781 ggcggattcc tgtcctggct ctggatcctg caggccatgc tgatcgcagt gatctttctc
      841 tcggccaact actacctgtg gattggcatg gggcgtattc agggaggcga acgctatcgg
      901 ggacgcatca aatggcttct cgcaatcctg accctgggcg tgttggtctg ggcaacgccc
      961 cataatccgg tcgtaacccc cgaggagcag gcactgattg gcggagcgtt ccacccgtta
     1021 ctcggtgtgc tgggggtgat gtcggcgaag aatacggccg tgaacttgat gatattgacc
     1081 accttcgtca gtttcttgtt ctatcgccgg gccggaaagg gcgagccgct tccttttgct
     1141 cgacagggaa acccggcgaa gatcgtcctg atcgtcgtgc tgctgatcac ggtcctgctg
     1201 agcctggcgc cggccttgta tgtgttgcga gatggcttgg gcgagacctc tcggctgttg
     1261 cgggtcggct tgtccgccct gcacgggctg ctgctgattg tgggcgtgat cctgaccttt
     1321 cgtgaccgtg gcaggctggg acaatggatc atcctgggct cggcggcgtc ggtggtgatc
     1381 ttctttgggg tctacggcta ttttgtggaa gccacaattc gaatcggctt ctcggtctat
     1441 caggtcctcg ccgtgttgac cgccttgatc agctcgaccg cgatcgacgt gttcctgtac
     1501 caaggcgcgc ccagccttgg aacgatcgaa tggggaaaga tcccggccag ggcccagtac
     1561 gtcttgatcc tgctggcggt cggattcacc tggctgatgg ggctgatggg ctacgcccga
     1621 aatgcgatcc gccagcactg gcacatttat gaggtcttgc aggacacctc ggtcgacgcg
     1681 ttctcgccga ccctcggttt cgctgcccga gtgatctcgg tctgcgtcct ggtgttcctg
     1741 gggctgatgg gcttcatctt ttggttgggc aacctcggcg agaagaaggc ccctgcggcg
     1801 ctccgtccgg agccgctccc cgcaactgac tgaatgggca attcgcttaa agtagccggc
     1861 ttcatcgtgc tggtcgtcgt cctcatcgct ggatttgctt cattgattcc gcagctggaa
     1921 tcgccggctc ctgaggagct ggtgatctcc ggcgagctct ccggcaccgc gctggccgac
     1981 gtcggagagg aagtattcaa ttcggccgcg gccggatgtc tggcctgcca cgggttgggc
     2041 aagccaggcc tgcgagctcc tgacctggcg gggatcgggg cagcggccgg ggatcgggta
     2101 gttgggctta gccctgagca atatttgcgg caggccctga ctgagccgtg cagctacgtc
     2161 gtctccggct acgactgcat catgcctcaa tctctgcagc agaccctcgg ggaggcgaag
     2221 ctcacggccc tagtcgcctt cctgcagagc ttgggaggcg aggtgaccgt ccgcttgtcg
     2281 gccgaggcag cccaggccgc cggcggtgaa gccggcggcg ggaccgccgg ggtcaccgcc
     2341 gcagaaatct tcaccaacgc cggctgcgtc gcctgccaca cccttccgat cgtgggtgcg
     2401 gccgggcagg tgggccccga tttgagcgag gtgggagcga ggctgactcc ggacgaagtc
     2461 cgccgctcca tcctgttccc cgatgagcag ctcgccgaga actgtccgac cggaccatgc
     2521 ttggcgggcc tgatgccgaa agcgttcggc gaatctctgt cgggtctcca attggaaact
     2581 ctggtgacgt tcctgagcga gcaaggggtg ccgtgagttt ggtccgcagg gtggtcggtc
     2641 ataagctagg tcaatttgcg ctggtattga tcggcgctta cctgctcttc cgcctcgggg
     2701 tcccctggct ctcgcggacc ctcacgggac agtcggcacc ggtaccggcg cacttgctgt
     2761 ggacgatcta catgccgatc gtactgctgg tgatgctgct gtttgtgtcg gccagtgaag
     2821 attcctggca ggaattccag cgcccacttc gggagctaat catcgaacag gagcgcgctc
     2881 cgctgatctg gacccggaga gtcgtgggga tcggcttacc gctgttggcc ggcctgtttg
     2941 cgtacctgca ggtgcgtccc gaggtcagcg cgcccccgga actgagatca gtgcaccctg
     3001 cgccggcttc cagaatcacg ctggatggcc aggcgatcga tctgcggacg gcggtcaatc
     3061 cgtttcgggg cgcaagcggg gccgccgatc cgactgctct tgcggaaggc aaggcgatct
     3121 acggccagta ttgcgtctac tgtcatgggg ataacttggc agggaacgga ctgttcgccg
     3181 gcagattacg accccgaccg gcggatttca ccgatccggg cacgatcgcc cagctgcagg
     3241 agtcgtatct cttctggcgg atcgccaccg gcggtccggg actgccgccg gaagccaagg
     3301 gttggaattc agccatgccg gcctgggaag gcaccctcgc ggcggacgat atctggaagg
     3361 taatccttta cctgtatgac gcgaccggcc agcaaccccg cgcagtaagt gagtcgtagg
     3421 tgagcactcg ggcgagatgg gttgccgcgt tgatccttgc cgccgtcggg ctggcggccg
     3481 cccgcagtcc gcatcaggcc aagcaatccc cggcccagca acccgcagcc ggcgaagcgg
     3541 tgtatcagca acgctgcgag atctgccatg gggcagccgg ggacggcaac ggggcggccg
     3601 cggcgaacat ggatccgagg ccgcgcgatt ttcgccgtgg ttggtacaag atccgcagtt
     3661 cggccaacgg gcaactgccg accgacgcgg acctgcttgc gatcatcacc aatgggatgc
     3721 ccggaaccac gatgccggcc tggggtgaaa ttctcagccc ggatgaactc caggcggtcg
     3781 ccggatacct gaaggggttc tcaagacgct tcgagcggga gacgccagaa ccagtctcgg
     3841 tcgttgcggc tccgggcagt tcggaggagc ggattggggg cggcgcgctg ctcttctccg
     3901 gcgcgcaggc ggaatgtgcg aagtgtcatg gaattgccgg acgcggggac ggcccgagcg
     3961 cgggcgagct cgtggacgac tttggccagc cgatcgtccc ggccgacctg accaccccgt
     4021 ggtcct
//
LOCUS       DATDPQ010000152         7558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3731267, whole genome shotgun sequence.
ACCESSION   DATDPQ010000152 DATDPQ010000000
VERSION     DATDPQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731267"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VI520_03560"
     CDS             <1..1002
                     /locus_tag="VI520_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLF36993.1"
                     /translation="KAQIRKELKSGEVLLLVGTHALLEDPVDFSKLGLAVIDEQHRFG
                     VSQRAALRSKGAAPHLMVMTATPIPRSLALTLYGDLDLTLIDEFPPNRIPIETRVLRP
                     IERSRAHSFIRGQLEQGRQAYIIYPLVEGSEHVQAKAAVDEHEKLQQSVFPEYRLGLL
                     HGRLGQEEKERVMTAFRSGELAALVSTSVIEVGIDVPNATAILIEGANRFGLAQLHQF
                     RGRVGRGAHPSFCLLIPETESETENARLQAMEQTADGFRLAELDLEQRGAGELFGTRQ
                     SGFELRAARLTDIRLIEKARNQAASLFARDPQLESPENQALALAVDRFWANGKGEIS"
     gene            1002..1520
                     /gene="coaD"
                     /locus_tag="VI520_03565"
     CDS             1002..1520
                     /gene="coaD"
                     /locus_tag="VI520_03565"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLF36994.1"
                     /translation="MVRAVFPGTFDPIHHGHIDIASRAARVFDELVVAVYNRPLKSLL
                     FSPQERLALVEQSFAGVKGVRVALFGGLIVNYCQEIQAQVIVRGLRVFSDFEHEFRMA
                     LANHRLAPDVEVVLFITQEEHSFLTGTTVREIAALGGNVSSMVPPHVVAALQRRFKEL
                     GDDALSPTSWRD"
     gene            1594..2079
                     /locus_tag="VI520_03570"
     CDS             1594..2079
                     /locus_tag="VI520_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF36995.1"
                     /translation="MDILHLVDRLEELFNESRPIPLTRSVLVDEDRVLEIIDQMRISI
                     PDEVKKAQQILAQRDRVMAQAQEEANRTLTLAKRQGEDSLAKDAIVRAAHERAEQILD
                     QARADVETVRRDSDEYVVEALSTLEDELIRLLNQARNGIAKVSAERQRQLPQEELAEQ
                     A"
     gene            complement(2140..3801)
                     /locus_tag="VI520_03575"
     CDS             complement(2140..3801)
                     /locus_tag="VI520_03575"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLF36996.1"
                     /translation="MTKYIFLTGGVVSSVGKGVTTAALGKLMKERGFAVSIQKLDPYI
                     NVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTDASLSRVCNLTSGQIYSNVIAQE
                     RRGDFLGGTIQTIPHITNEIKARIREVGQVTGAELVLVEVGGTVGDIESLPFLEALRQ
                     IRSEIGRENSLFVHVTWLPYIGATGELKTKPTQHSVRELRSIGISPDVIVARSDYPVG
                     ESLREKIALFCDVEPKAVIPVITTDILYEVPLRLEAQGLGDYITHRLGLSESKRPDWS
                     EWERLVAQERTDRPRVCVAIVGKYVELQDAYMSVREAVRHASLAVGVETELLWLHSAE
                     LEKGKGWEELRRAHGIIVPGGFDHRGIEGKISAARYARQSQVPYLGLCLGMQAMVIEF
                     GRHVLGSEDTNSTEFDPHTPHPVIDLLPEQRGIGDKGGTMRLGLYPCELVPGTAAQRA
                     YAVPAIEERHRHRFEFNNTYREILAEHGMVYSGMSPDGKLVEIAELADHPFMLGSQFH
                     PEFLSRPTRPHPLFRAFIEAVAERAGVKTRTAGEPAPHSEERVET"
     gene            complement(3893..4267)
                     /locus_tag="VI520_03580"
     CDS             complement(3893..4267)
                     /locus_tag="VI520_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HLF36997.1"
                     /translation="MEKTRTDHMVDRLRDLQGRTPDIEASAIVSVDGLIMASSLPGGV
                     EEDRVSAMSAAMISLGERIATELGRGMLDEVYIHGEGGYVLLMSSGQEAVLTVLARKK
                     AKLGLVLLEMRRAVGDLERLVG"
     gene            complement(4305..4853)
                     /gene="rimM"
                     /locus_tag="VI520_03585"
     CDS             complement(4305..4853)
                     /gene="rimM"
                     /locus_tag="VI520_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HLF36998.1"
                     /translation="MSDSRLVAPLNLSAPQHLAVGRVLRPHGVRGDLVVDAYSGRLAA
                     VEPDSEVFLGEPGSSHSVISIRPHQGRILLRLADCSDRDQAEQFRGLEVRILMDRTGP
                     LPAGVYYHSQILGLEVVEVGGRGLGEVSEILETGGNDVYVVRAANGTELLLPAISSVI
                     LDIDLEAKQIRVAIPQGLEEGK"
     gene            complement(4846..5073)
                     /locus_tag="VI520_03590"
     CDS             complement(4846..5073)
                     /locus_tag="VI520_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HLF36999.1"
                     /translation="MKELVEFIAKSLVDDPTQVRVTETEGPREVRIELRVAEGDQGRV
                     IGRNGRIANSIRSLLRVIASRKGVRSSLDIV"
     gene            complement(5084..5572)
                     /gene="rpsP"
                     /locus_tag="VI520_03595"
     CDS             complement(5084..5572)
                     /gene="rpsP"
                     /locus_tag="VI520_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLF37000.1"
                     /translation="MVRIRLRRVGSRNQVSWRIVAADRESPRDGRFLEILGSYNPRTK
                     PATVLVDEARLFHWMTHGAVPSDSLLKILKPIGSWDRLERFKQGEDLDKLVQEAKAGL
                     ATIDPRTRRDDLVGQRSAKKQRPKKDSGRPKVAPSPQPVSESSNPVVVESSEASGEAP
                     AE"
     gene            complement(5604..>7558)
                     /locus_tag="VI520_03600"
     CDS             complement(5604..>7558)
                     /locus_tag="VI520_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLF37001.1"
                     /translation="LLALLASQIYVAYGRAAILYGTLGLLGAGAGIGVLKLEVVRTRF
                     EAWINPWLDPTGAGYQIVQSLIAIASGGLLGSGPGRGAPTAVPVVQSDFVFAAVAEEW
                     GLVGALAMIGLFAVLVGRGLRVAAGARDPFAVLLAVGVSLALGLQTLLILGGTLRLLP
                     ITGVTLPFVSYGGTSFVTSSIAAGFLVLLSSERHPGLGAFGRPILRAQAVLSAGWIAL
                     ALALGWWVIVRGEALTTRMDNPRRALASRYSLRGSIFDWEGVELAASMGTIGSFARVY
                     YDPASAPVTGYDSSLYGQAGIETTMDGFLRGEIGPDPLSIWWSELVYGTPPPGLHLRL
                     TLNSELQRYAAEGLAASRGAVVVVDARSGSILAMASSPSFDPSTLDQVWPELVVRTDG
                     PLLNRATQGRYQPGMTLAPLLFAWGISNGLIEAEGPAPDFGGLVTVFDRDLGCERPID
                     PQREPTWATALSLACPGPFAALGEAMGSEELLRALKAFGLLDPPLIRLEVAPAAGTGL
                     TGDPGLAAIGQADLTVTPLQLARAFSVLTGAGVRPALTLVAAVGPTPGDWQPLPPLDD
                     AQPVLPPSEAQRTLDNLTDHGAGLKGYEVQAASGSGSLAWFIGFGAGDELTVVLLEGT
                     SPGAARELGLGLLRLAADLDLTSREG"
BASE COUNT         1430 a         2523 c         2272 g         1333 t
ORIGIN      
        1 aaggctcaaa tccgtaaaga gctcaagagc ggcgaggtct tgctgctcgt cgggactcac
       61 gccctgcttg aagacccggt cgatttctcc aagctggggt tagcggtgat cgacgagcag
      121 caccggttcg gcgtatcgca gcgggccgcc ctgcgcagca agggcgcggc cccgcacctg
      181 atggtgatga ccgctacccc gatcccacgc tctttggccc tgaccctcta cggggatctg
      241 gatttgaccc tgatcgacga gtttcccccc aatcgcattc cgatcgagac tcgggtgctg
      301 cgccccatcg agcggtcccg ggcccacagc ttcatccgcg gccagttgga acagggcagg
      361 caggcctaca tcatctatcc gctagtcgaa ggatcggaac atgtccaggc caaggcggcc
      421 gtggacgagc acgagaagct ccagcagtcg gttttcccgg agtatcggct tggattgctc
      481 cacggacggc tggggcagga ggagaaagag cgggtgatga cggcctttcg gtctggcgag
      541 ctggcggcgc tggtctctac ctccgtaatc gaggttggga tcgacgtccc caacgctact
      601 gcgatcctga tcgagggcgc caaccgcttt ggactggccc agctccacca attccgcggt
      661 cgggtcgggc gcggcgcgca tccttccttc tgtctcctga tcccggagac cgagtccgaa
      721 accgagaatg cccggctgca ggccatggag cagaccgcag acggcttcag gctggccgag
      781 ctcgacctcg agcagcgcgg ggccggtgag ctgttcggca cccggcagtc cgggttcgaa
      841 ctgcgggccg cccggttgac cgatatccgc ctcatcgaga aggctcgcaa tcaggccgcg
      901 agtctcttcg cccgcgaccc acagctcgag tcgccggaaa accaggcgct cgcgctggcc
      961 gtggatcgat tctgggcgaa cggaaaggga gaaatcagct aatggtccga gccgtatttc
     1021 ccggaacctt tgatccgatt caccatgggc acatcgacat cgcctcccgg gcggcccgag
     1081 tttttgacga gttggtcgtg gcagtctata accgcccgct caagagcctg ttgttctccc
     1141 cgcaggagcg gctggcgctg gtcgagcaga gctttgccgg agtaaagggc gtccgagttg
     1201 cgttgttcgg cggcctgatt gtcaactatt gccaggagat ccaggctcag gtcatcgtgc
     1261 gcgggcttcg ggttttctcc gacttcgagc acgaattccg aatggcactg gcgaaccatc
     1321 ggctcgctcc ggatgtggag gtggtgctct tcatcaccca ggaggagcac agcttcttga
     1381 ccggcacgac cgtgcgtgaa atcgctgcgc tgggcggcaa cgttagcagc atggtccccc
     1441 ctcacgtcgt agccgccctg caacggcgtt tcaaggagct gggggacgat gccctgtcgc
     1501 cgacctcctg gcgcgattga ccccagttga ccagaattcc agttcggtgt actcttcgac
     1561 ccttgtgacc acgggctagc gggaggaccc ggaatggata tcctgcatct ggtggaccga
     1621 ttggaagagc tcttcaacga gagccgaccc attcctttga cccgcagcgt actcgtcgac
     1681 gaggatcggg tgctcgagat tatcgaccag atgcgcatct cgattcccga cgaagtgaag
     1741 aaggcccagc agatcctcgc gcaacgtgat cgagtgatgg cccaggctca ggaagaagca
     1801 aaccgcaccc tgacccttgc gaagcgccag ggcgaggatt cgctcgccaa ggacgcgatc
     1861 gtccgggccg cccacgaacg ggccgagcag atcctggatc aggcccgggc ggacgtcgag
     1921 actgtccggc gcgactccga tgaatacgtc gtcgaggccc tttccacctt ggaggatgag
     1981 ctgattcggc tgctcaatca agcccgcaac gggatcgcaa aagtcagcgc cgagcgccag
     2041 cgccagctcc cgcaagagga gcttgccgag caggcctagc aactccgagc cctcgacaga
     2101 gttggaatcc aatcgcgccc ttttgggcgc gattcctttc tacgtttcca cccgttcctc
     2161 ggaatggggc gctggttcgc cggccgtccg ggtcttgacc cccgcccgct ctgccacggc
     2221 ctcgataaac gcccgaaaga gcggatgggg gcgggttgga cgcgagagga actcgggatg
     2281 gaattggctg cccagcatga agggatggtc ggccaattcg gcgatttcaa ccaacttccc
     2341 atctggcgac atcccagagt agaccattcc atgctcggcg aggatctccc gataggtgtt
     2401 gttgaattca aaccggtggc gatgacgctc ttcaatcgcg ggcacagcat aggcccgctg
     2461 ggcagcggtc cccggtacca gttcgcacgg atacagcccc aacctcatcg taccgccctt
     2521 gtccccaatt ccgcgctgct cgggaagcag gtcgatcacc gggtgggggg tgtgagggtc
     2581 gaattcggtc gaattggtgt cctccgaccc gaggacatgg cgcccgaatt cgatcaccat
     2641 cgcttgcatg ccgagacaga gtccaagata cggaacctga ctctggcggg cgtagcgggc
     2701 cgccgagatt ttgccctcta tcccacggtg gtcaaacccg cccggcacaa tgatgccgtg
     2761 ggcacgtctg agctcctccc agcccttgcc tttctcgagc tccgccgaat gcagccacag
     2821 cagttcggtc tcgaccccta cggccagtga ggcatgccgc accgcctctc gaacgctcat
     2881 gtacgcatcc tgcagctcta catacttccc aacgatcgct acgcacaccc gggggcggtc
     2941 cgtgcgctcc tgcgccacca gccgctccca ctccgaccaa tcgggccgtt tggactcgct
     3001 cagtcccagc cggtgggtga tgtagtcgcc cagcccctga gcctcgagcc ggagcggcac
     3061 ttcgtacaag atatcggtgg taatgaccgg gatgaccgcc tttggttcta cgtcgcagaa
     3121 tagcgcgatc ttttctcgca aactctcccc caccgggtaa tccgaccggg cgacgatcac
     3181 gtccggtgag atgccgatcg agcgtagctc ccggaccgag tgctgagtcg gtttggtctt
     3241 gagctcgccg gtggccccga tatatggaag ccaggtgaca tggacaaaca gcgaattctc
     3301 gcgaccgatc tccgatcgga tctgccgcag ggcttccagg aagggcagtg attcgatgtc
     3361 gccgaccgtc ccgccaactt cgaccagcac gagctccgct ccggtcacct ggccgacctc
     3421 ccggatgcgg gccttgatct cgttggtgat atggggaatg gtttggatcg ttccacccag
     3481 gaaatctccc cgccgctcct gagcgataac attggaatag atctgcccgc tcgtcaggtt
     3541 acacactcgg ctgagactgg catcggtgaa gcgctcgtaa tgtccgagat ccagatcggt
     3601 ctccgaaccg tcgtccaaga cgtacacttc cccgtgctgg tacgggctca tggtccccgg
     3661 atcgacgttg atatacgggt ccagcttctg gatcgagacc gcgaagccgc gttccttcat
     3721 cagcttgccg agggccgccg tggtgactcc cttgccaacc gaactcacca ccccgcctgt
     3781 cagaaagatg tacttggtca tggcatcctc ccccataaag ggcggtgggg agtgtcgccc
     3841 cccggcgaac tccccaccgt cagaaactaa acggattacg acactccaac cctcatccga
     3901 ctagccgctc cagatcgcca accgcccgcc gcatctcaag caggaccaat ccaagcttag
     3961 cctttttcct ggccaggacg gtcagcacgg cctcctggcc ggaggacatc aacaacacat
     4021 aaccaccttc gccgtggata tagacctcgt ccagcatacc gcggcccaat tcggtcgcga
     4081 tccgctcacc gaggctgatc atcgccgccg acatcgccga gactcgatcc tcttcgaccc
     4141 caccgggcag cgaggacgcc atgatcaagc catccacgct gacgatcgcc gaggcttcga
     4201 tgtccggagt cctgccctgc agatccctca gccggtcgac catgtggtcg gtccgggtct
     4261 tttccatcag cgctcacccg gccggaacgc gggacagtct accatcattt cccctcctcc
     4321 agcccctgtg ggattgccac tcggatttgc ttcgcctcga gatcgatgtc caggatcacg
     4381 gaagaaatcg cgggaagcag cagttccgta ccgttggccg ctcgcacgac gtacacatcg
     4441 ttgccgccgg tctcgaggat ttcgctgacc tcacccagac cgcgacctcc cacttccacg
     4501 acctccaggc cgaggatctg cgagtggtag tagaccccgg cggggagcgg gccggtccgg
     4561 tccatcaaga tacgaacctc caggcctcgg aattgctccg cctgatcccg atcggagcag
     4621 tccgccagcc tcaataggat tcgcccctgg tgaggccgaa tcgagatcac ggaatggctt
     4681 gaccccggtt cgccgaggaa gacctcgcta tccggctcta ctgccgccag gcgacccgag
     4741 taggcatcca ccaccagatc gcctcggacg ccatgcggac gtaaaacgcg cccgacagcc
     4801 aagtgctgag gggcgctcag attgagcgga gcgaccaggc gggaatcaga caatatccag
     4861 cgagcttcga actcccttgc gggatgcgat taccctcaac aaggaccgga tagagttggc
     4921 gatccgtccg ttgcggccga tcacccgtcc ctggtcacct tcggcgaccc gcagctcgat
     4981 ccgtacctca cgcggtccct cggtttccgt cacccggacc tgagtggggt catcaaccag
     5041 cgactttgcg atgaattcaa cgagttcttt caagatcccg gccctattcg gcgggtgctt
     5101 ccccgctcgc ttccgaactc tcaaccacca ccgggttgga gctctcactg accggctgtg
     5161 gactgggggc aaccttgggc cgcccactgt ccttcttcgg gcgctgcttc ttagccgagc
     5221 gctgcccgac caaatcgtcc cgccgggtgc gcgggtcgat ggttgccagg cccgctttgg
     5281 cctcctggac cagtttatcc agatcctcgc cctgcttgaa ccgttccaag cggtcccagg
     5341 acccgattgg cttgagtatc ttcagcagcg agtcagaggg cacggctccg tgggtcatcc
     5401 agtgaaacag ccgcgcttca tcgaccaaca ctgtggcggg tttcgtgcgc gggttatagg
     5461 aacccaggat ctccaggaat ctgccgtccc gcgggctttc tcgatcggcc gcgacgatcc
     5521 gccagctgac ctggttgcgg cttcctaccc gacgtagtct gattcggacc atatctcgct
     5581 ttccatgaag acctccggtg attttagcct tcgcgggagg tgaggtcaag gtcggcggcc
     5641 aggcgcaaca gaccgaggcc gagctcgcga gccgcgcccg ggcttgtacc ctccagcagc
     5701 acaaccgtca gttcgtctcc tgccccgaat ccaatgaacc aggcgagcga cccggatccg
     5761 ctcgcggcct gcacttcata tcccttcagt ccagcaccat gatcagtcag gttatcaagg
     5821 gtccgctggg cctcgcttgg cggcaagacc ggctgcgcgt cgtcgagcgg cggcagcggc
     5881 tgccagtccc ctggcgtcgg tccgacggcc gccaccaggg tgagagcggg tcgcacccct
     5941 gccccggtta agaccgagaa tgcccgggca agttggagcg gagtcacggt caggtcggcc
     6001 tgcccgatcg cggccaaccc ggggtccccg gtcaagcccg tcccagccgc gggagcgacc
     6061 tccaaccgga ttaacggcgg gtcgagcaag ccgaaggcct tcagcgcccg aagtagctct
     6121 tcggatccca tcgcctctcc gagcgccgcg aatggcccgg gacaggccaa ggaaagagct
     6181 gtcgcccagg tgggctcgcg ctgcggatcg attgggcgtt cacatcccag gtcccggtcg
     6241 aagacggtca ccaggcctcc aaaatccggg gcgggacctt ccgcctcgat cagtccattc
     6301 gaaatgcccc aggcgaatag gagcggagcc agcgtcatcc cgggttgata tcgaccctgg
     6361 gtcgctcgat tgagcaacgg gccatccgtc cgaaccacca actccggcca gacttgatcc
     6421 agggtactcg ggtcgaagct tggcgaagag gccattgcca ggatggaccc ggaccgtgcg
     6481 tccaccacga ccaccgcacc ccggctggcg gccagtccct cggccgcgta gcgctgcagc
     6541 tctgaattca aggtaagcct gagatggaga ccgggcgggg gcgtgccata aaccagttcg
     6601 ctccaccaga tcgatagcgg gtccgggcca atctcacccc gcaggaaccc atccatggtg
     6661 gtctcgattc cagcctggcc gtagaggctg gaatcgtacc cagtgaccgg agcgctggcc
     6721 gggtcgtaat agacccgcgc aaacgaaccg atcgtgccca tggaggcggc cagctcgacc
     6781 ccttcccagt cgaagatgga gccgcgcagc gaatagcgac tggcgagcgc ccggcgcggg
     6841 ttatccatgc gtgtagtgag cgcctcgccg cgcacgatca cccaccagcc cagcgccagt
     6901 gcgagggcga tccagccggc cgaaaggacg gcctgggcgc gcaggatggg ccggccgaaa
     6961 gcgcccagac cggggtgtcg ttcgctggaa agcaagacca aaaacccggc tgcgatggag
     7021 ctggtgacga atgacgtgcc tccataggat acgaagggta gggttacgcc cgtgatcgga
     7081 agcaggcgca gcgtgccgcc cagaatcaag agagtctgca gcccgagcgc aagcgatacc
     7141 ccgactgcca gcaggaccgc gaacggatcc cgtgccccgg ccgcaactcg caggccgcgg
     7201 cccacaagca cggcgaacag cccaatcatt gctagagccc caaccaggcc ccactcctcg
     7261 gcaacggctg cgaatacgaa gtcggattgg actaccggca cggcagtcgg ggccccgcgc
     7321 ccggggccgg aacccagcaa acccccgctg gcgatggcaa tcagcgactg gacgatctgg
     7381 tatccagcac cggttgggtc gagccagggg ttgatccagg cctcaaatcg ggtccggacc
     7441 acttccagtt tgagaacccc gatcccagct ccggcgccca gcaggcccag cgtgccgtac
     7501 aagatcgccg cgcgtccgta agcgacgtag atctggctgg cgagcagcgc aagaagca
//
LOCUS       DATDPQ010000153         4485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1886100, whole genome shotgun sequence.
ACCESSION   DATDPQ010000153 DATDPQ010000000
VERSION     DATDPQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886100"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VI520_03605"
     CDS             complement(<1..811)
                     /locus_tag="VI520_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HLF37002.1"
                     /translation="MRIHLVDGTYELFRSFYGVPSKKAPDGREVGATVGLLNSLLLLL
                     TKEGATHVGCAFDHVIESFRNDLYPGYKTGEGIDPDLLAQFPLAERAVAALGLIVWPM
                     VEFEADDALASATVRFRRRRSVEQVVLCSPDKDLAQLVSGDRVVSWDRRREIIYDEAA
                     VVEKFGVSPGSIPDWLALVGDAADGYPGIPAWGEKSASAVLSKYEHLETIPKDHRKWG
                     LPAGRALRLAENLNTHREDAFLFRRLATLRTDVPLEENLKDLEWRGARKRLR"
     gene            complement(856..1410)
                     /locus_tag="VI520_03610"
     CDS             complement(856..1410)
                     /locus_tag="VI520_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37003.1"
                     /translation="MPDNAELDSLKSSMPSARGLFGLGVLAWISMLGVDFFVHAGLLA
                     RLYAQPSAFLLSPEDAFRLIPLGYLSFLLVSALILWLMRRLEIGTWQRGLSSGLLIGG
                     LMWGALAIGLLSISTASPALMFGWFAGQTVELGVAGAVVGSGLGGTPVRKLLARVTAL
                     ILVLAVVTITLQSIGWSPAIRIGE"
     gene            complement(1400..2179)
                     /locus_tag="VI520_03615"
     CDS             complement(1400..2179)
                     /locus_tag="VI520_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37004.1"
                     /translation="MSSTQGDERLELLTQIARTYGLRQRFAEAHELLKEVERELAGAG
                     PRPRIRYLLERGRTHNSGGEEEKARALFVEAWERAQTAQQEGLAVDAAHMVAITFSGR
                     PEAIEWNQRGLELARRSEDPKALALIPAMLNNRGWDLHDMGRFAEALPVFEEALAVWL
                     VRGKLKQILIAKWAVARCLRSIGRHADALAILRELEAEHAATESVDGTVFEEIAENLV
                     ALDEHNDAQPYFGRAFDELSKDEWFVKNEPSRLAGMQARAG"
     gene            complement(2254..3702)
                     /gene="pyrF"
                     /locus_tag="VI520_03620"
     CDS             complement(2254..3702)
                     /gene="pyrF"
                     /locus_tag="VI520_03620"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HLF37005.1"
                     /translation="MIAPRPFMLSLTECANRADSLLCVGLDPHPELLSEASAPGAAEF
                     CLRLIEATAEYACAFKPNSAFFEAYGAPGWTALREVIAAVPEGIPVILDAKRADISST
                     ARAYAQAAFGWLGADALTVNPYLGHDAVEPFLEDPGRGVFLLCKTSNPGAGDLQSLSL
                     GGEALYLKVARLASDWNAVGNLGLVVGATDPQALAAVRELAPDLWILAPGVGPQGGDM
                     EAALVSGLREDGAGLLVAVSRAIAQAESPAQEAARMKAQVNALRESVGSRPGRTSRPP
                     SHIPELSPGLASLAEGLLKADCVQFGDFKLKSGEQSPIYFDLRRLTGDPELLRRVADA
                     YFPLLTGLSFDRLAAIPYAGLPIATALSLQSGVPLVYPRKELKEYGTGAVVEGGFKPG
                     EVAVLIDDLATTGGSKFEAIERLGAAGLTVRDVVVLIDRQAGARQALAEAGQRLHTVF
                     TLSQLLSQWELVGAVPVDDLANVREYMAREQQ"
     gene            complement(3729..>4485)
                     /gene="pyrB"
                     /locus_tag="VI520_03625"
     CDS             complement(3729..>4485)
                     /gene="pyrB"
                     /locus_tag="VI520_03625"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HLF37006.1"
                     /translation="TSSSFLAAMERLGGSVIPINEVTYSSVAKGESLPDTIRTLQAYA
                     DVIVLRHPELGSAKLAAQYASKPIINAGDGVGEHPTQALLDLFTIEEELGRVDGLTVT
                     MLGDLKYGRTVHSLARLLASFEVRINYVSPEILPMPEEVVSELEHRGIPQARAASLEE
                     VLPQTDVLYVTRVQKERFQDLAQYEQVKGAYVITPETLQPAKDEMIVMHPFPRVGEIS
                     VEVDTDPRAAYFRQMEYGLYVRMALLAMVLGKA"
BASE COUNT          889 a         1444 c         1339 g          813 t
ORIGIN      
        1 ttctcagtcg tttccgcgca ccgcgccatt ccagatcttt gaggttttct tcgaggggga
       61 cgtcggtgcg aagagtcgcc aggcggcgga aaaggaaggc gtcctccctg tgggtgttga
      121 gattctctgc gaggcggagg gcgcgaccag ccggcaggcc ccacttgcga tgatctttcg
      181 ggatggtctc gaggtgctcg tatttggaga gcaccgcgga ggcggatttc tcaccccaag
      241 cggggatccc agggtagccg tccgccgcgt cacccaccaa cgcgagccag tcggggatgg
      301 accctggcga aacaccgaac ttctcgacga ccgcggcttc gtcataaatg atctctcggc
      361 ggcggtccca actcacgacc cggtccccgg agaccagttg agccaggtct ttgtcgggtg
      421 agcagagcac aacttgttcg acggagcgcc tacgccggaa gcgaacggtc gccgaggcga
      481 gtgcgtcatc ggcctcgaat tcgaccatcg gccagacaat cagacccaag gccgccacgg
      541 cccgctcggc gagcgggaac tgggccagga ggtccgggtc gatgccttcg ccggtcttgt
      601 atcccgggta aaggtcattg cggaacgact cgatgacgtg atcgaacgcg cagccgacat
      661 gggtcgctcc ctccttggtc aggagcagga gcaggctgtt gagcagaccg accgtcgcgc
      721 ccacctcacg cccatcgggc gcctttttcg agggcacgcc gtaaaagctg cggaatagtt
      781 cgtaggtgcc gtcgacgagg tggattctca ttagttgcga accctccagc aaggattagg
      841 cggattgccc atttgctatt cgccaatcct gatcgccggc gaccaaccga tactctggag
      901 ggtgatcgtg acaaccgcca atactaggat tagagcggta accctcgcaa ggagcttgcg
      961 aactggcgta cctcccaggc cgctgcctac tacagcccct gctacgccta gctcaacggt
     1021 ttgaccagca aaccaaccaa acatcagagc gggacttgcc gtcgagattg agagcaagcc
     1081 tatcgccagt gctccccaca tcaagccgcc aatgaggagg ccagagctga ggccacgttg
     1141 ccaggtgccg atttctagtc tcctcatgag ccaaagaatc aatgcactca ccaacaagaa
     1201 ggacaaatag ccgaggggga tcaatcgaaa tgcatcttcg ggtgaaagca ggaaggcgct
     1261 cggctgggca tatagccgtg caagcaatcc agcgtgcacg aagaaatcga ctccaagcat
     1321 cgaaatccac gccagaacgc caaggccgaa aaggcctcga gctgaaggca tggaactctt
     1381 gagcgagtct aactcagcgt tatccggcac gtgcctgcat tcctgccagc ctcgatggct
     1441 cgttcttcac aaaccactca tccttgctca actcgtcgaa tgctcttcca aagtagggtt
     1501 gagcgtcatt gtgctcatcg agtgcaacaa gattctccgc tatctcttca aacacggttc
     1561 catcaaccga ctccgtggct gcatgctcag cttcaagttc ccgcaagatc gctagagcgt
     1621 cggcatggcg cccaatggaa cggaggcagc gagcgacggc ccacttggca atcaagattt
     1681 gcttgagttt gccacgtaca agccacactg ccaatgcttc ctcaaacacc ggcaaggcct
     1741 ccgcgaagcg tcccatatcg tgcaagtccc acccgcggtt gttgagcatc gctggtatga
     1801 gagcgagggc ttttggatcc tcagaccggc gtgcgagctc cagaccgcgc tgattccatt
     1861 ctatagcctc cggccttccc gaaaacgtga tcgccaccat gtgtgccgcg tcgacagcca
     1921 gaccctcctg ctgcgcggtc tgtgcccgtt cccaggcttc gacaaataat gcacgcgcct
     1981 tttcttcttc gccgccggaa ttgtgcgttc gcccccgttc gagcaaatac cgaatgcgcg
     2041 gtcgtggacc ggccccggca agttccctct cgacctcctt taggagttcg tgggcctcag
     2101 cgaagcgctg gcggagccca taagttcgag caatttgagt gagcagctcg aggcgctcat
     2161 cgccttgggt tgaactcaac aaactgcgaa atcggtcttc gctggaggcg gggtctgtat
     2221 actcccagaa ggagtcgatg tcgattgaat tccctattgc tgctcgcgag ccatgtattc
     2281 tctcacgttg gcgaggtcat ccacgggcac cgcgcctacg agctcccact ggctgagcag
     2341 ctgggagagg gtgaagaccg tgtgaaggcg ctggccggcc tcagccagag cctggcgcgc
     2401 cccggcttgg cgatcgatca acacgacgac atcccgcacg gtcaggccgg ctgccccgag
     2461 tcgttcgatg gcctcgaact tgcttccgcc ggtggttgcc aggtcgtcaa tgagcacggc
     2521 gacctcgccg ggcttgaatc cgccttcgac cactgcaccc gtcccatact ccttgagctc
     2581 cttacggggg tagaccagcg gaacgcctga ctgcaacgac aacgctgtcg caatcggcag
     2641 gccggcgtag ggaatggcgg ccagccgatc gaaagacagc ccagtcagca gcggaaagta
     2701 ggcatccgcg actcggcgga gtagttccgg atcaccggtg agccggcgca gatcgaagta
     2761 gatcggcgac tgctcgcccg acttcagctt gaagtccccg aactgaacgc agtccgcctt
     2821 cagcagcccc tcggcgaggc tcgccagccc gggcgagagc tccggaatgt ggctcggagg
     2881 ccggctggtt cgcccaggcc gggacccaac gctttcacga agcgcgttca cctgtgcctt
     2941 catccgggct gcctcctgcg caggagactc agcctgggcg atcgcccgcg agactgcgac
     3001 caatagtccc gccccgtcct cccgcagtcc cgataccagc gccgcctcca tatcgcctcc
     3061 ctgcgggccg acccccggtg ccaggatcca caggtccggc gccagttccc ggaccgcggc
     3121 cagcgcctgc ggatcggtcg ccccgacgac caaccccaag ttacccactg cattccaatc
     3181 gctcgccaag cgggcaactt tcagataaag agcttcgccg cccagcgaaa gggactgtag
     3241 gtctccggcg cccggattgg aggtcttaca cagcagaaag actccccggc ccggatcttc
     3301 caggaagggt tcaaccgcat cgtggccgag atacgggttc accgtcaagg catccgcacc
     3361 gagccagccg aaggccgcct gggcatacgc ccgggcggtc gaggagatgt cagcccgctt
     3421 ggcgtcgagg atcaccggaa tgccctccgg gactgccgcg atcacttccc gcagggcggt
     3481 ccagcctggc gcgccgtaag cttcgaagaa tgcgctgttg ggcttgaagg cgcaggcgta
     3541 ctcggcggtc gcctcgatca atcgcaggca gaactcggcc gccccagggg cggaggcttc
     3601 cgagagaagt tcggggtgcg gatccagacc gacgcagagc agtgaatcgg cccggttggc
     3661 acactcggtc aagctcagca tgaacgggcg gggagcaatc atcgcctgac gaattcgcag
     3721 aggcgcggct aggccttccc cagcaccatg gccaagagtg ccatccggac gtagagcccg
     3781 tattccatct gccggaaata ggcggcccgt gggtcggtgt cgacctcgac actgatctcg
     3841 ccgacccggg ggaatgggtg catcacgatc atctcgtcct tagccggctg cagcgtctcg
     3901 ggagtgatca cgtacgcgcc tttgacctgt tcatactgcg cgagatcctg gaagcgttcc
     3961 ttctgcaccc gggtgacgta cagcacatcg gtctgtggga gcacctcctc gaggctcgcg
     4021 gccctagctt gcggaatgcc tcggtgctcg agttcgctca ccacttcctc cggcatcggc
     4081 aggatctccg gtgagacgta attgatccgg acctcgaacg aggcaagcaa ccgggccaac
     4141 gagtgcacgg tgcggccgta ctttaggtcg cccagcattg tgaccgtcag gccgtccact
     4201 cggcccagct cttcctcgat cgtgaagaga tcgagcagcg cctgggtcgg atgttccccc
     4261 actccgtcac cggcgttgat gatcggcttg ctcgcgtact gggccgccag cttggccgaa
     4321 cccaactccg gatgccgcag cacaatgaca tcggcgtaag cctgcagggt ccggatggtg
     4381 tcgggaagcg actcgccctt ggcgaccgaa gagtaggtga cctcgttgat cggaattacg
     4441 ctgccgccca accgttccat cgccgccaaa aaggaggatg aggtg
//
LOCUS       DATDPQ010000154         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2677805, whole genome shotgun sequence.
ACCESSION   DATDPQ010000154 DATDPQ010000000
VERSION     DATDPQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677805"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..789)
                     /locus_tag="VI520_03630"
     CDS             complement(139..789)
                     /locus_tag="VI520_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HLF37007.1"
                     /translation="MTYIVIGCGRVGAELADRLSQRGFEVSILDQEPAAFLSLSPDFK
                     GRTLEGDALDRHLLERAGIREAQALAAVTDSDALNAVVGHIAKTAYRVAHVVVGNFDP
                     KHRPMFEAFGLQTVSAGTWGAQRIEELMVHGGPQVVYEAGNGEIGVYELRIPPGWNGR
                     PLQDLLAGSQSLPVAVTRAGRAELPGAGTRLQTGDVVHVSATLDGVQAIEARLAGS"
     gene            complement(817..>2647)
                     /locus_tag="VI520_03635"
     CDS             complement(817..>2647)
                     /locus_tag="VI520_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLF37008.1"
                     /translation="APHQTIGKAIGLAVFASDALSSTAYATQEILVILVVAGTAALGY
                     ALPIAMGIVGLLAIVTISYQQTIHAYPGGGGAYIVARDNLGELPAQTAGAALLTDYVL
                     TVAVSVSSGVAQIVSAYPSLSTNRVAIAVGMVTIIMIVNLRGVRESGRAFAIPTYFFL
                     LMMVLTLGTGLVRAVTGTLGSVIDPPALEFGETLLAVTPFLILHAFSSGTTALTGVEA
                     ISNGIPAFKEPRSRNAAITLIWMSVILGTLFLGITFLAGEIHALPSEGETVISQLART
                     IFDSRGTLYLMLIAATTIILIMAANTSFADFPRLSALVAGDGFLPRQFTYRGSRLVFS
                     RGIVALALLASLLILIFQARVTALIPLYAIGVFVSFTLSQAGMARRWWKIGHLQSGQV
                     VQERGSTLSYTPNWQLKMLINGFGAVCTAVVTLVFTFTKFREGAWVVIISAPVLVLIF
                     TAIHHHYRDLAKNLSVEDYGPPPQVRRHRVILPISGVHRGTLDALRYATSLSDDVTAV
                     HVSIDPEEAERVRRKWETWGGGVRLVILESPYRLFIEPLISYIEEVMAQRQNHDTVTI
                     VVPQFVSRRWWHNLMHTQTALFLRLALMFRPGIVITDVPYHVE"
BASE COUNT          531 a          823 c          859 g          434 t
ORIGIN      
        1 tcggtcggca gggctcggtg caggcggcgc agcaccagcg gacggctttc gatcagccgt
       61 acctggtgct gctgttcaac gagcaaagcg gccaagcggg cgccggttcg cccgccgccg
      121 gcgatgacga ccaacatctc agctccctgc cagccgggct tcgatggcct gaaccccgtc
      181 caaggtggcg ctcacgtgta cgacgtctcc cgtctgtagc cgggtgccag ctccgggaag
      241 ctcggctctg ccagcccggg ttaccgccac cggcagagac tgcgagccgg ccagcaggtc
      301 ttgcagcggt cggccgttcc agccgggtgg tatcctgagt tcgtagaccc cgatctcgcc
      361 attgccggcc tcgtaaacca cctgtggccc accatgcacc atcagttcct cgatccgctg
      421 ggcgccccag gtcccggccg aaacggtctg caatccgaag gcctcgaaca tcggacgatg
      481 cttagggtcg aagttgccca ccaccacgtg ggcaacccga taggcggtct tggcgatatg
      541 gcccacgacc gcgttcagcg catccgagtc agtcacggcc gccaaggcct gagcctcccg
      601 gataccggcc cgctccagca agtgccgatc cagcgcatcg ccctcgagag tacgcccttt
      661 gaaatccggc gacagactca ggaaggccgc aggttcctga tcgaggatcg agacttcgaa
      721 gccgcgttga gagaggcggt ctgccagttc ggcgcccacc cggccgcagc cgatcacaat
      781 gtaggtcaca ggctcatttc ccgttgtcgg cccgggtcat tctacatggt acggaacgtc
      841 ggtaatcacg attccgggcc ggaacatcag cgccagccgg aggaacaacg cagtttgggt
      901 gtgcatcaga ttgtgccacc agcggcggga cacgaattgc ggaacgacga tcgtgaccgt
      961 gtcgtggttc tggcgttggg ccataacttc ctctatgtag ctgatgagcg gctcaataaa
     1021 gaggcggtag ggtgactcga ggatgaccaa ccgcacgcct cccccccaag tttcccactt
     1081 gcggcgaacc cgctccgcct cttccggatc gattgagaca tggaccgcag tgacatcgtc
     1141 cgacagcgag gtcgcgtagc ggagcgcgtc caaggtccct cggtggaccc cgctgatcgg
     1201 caggatgacc cggtgccgcc tcacctgtgg cggaggcccg tagtcctcca ctgacagatt
     1261 cttggccagg tcccggtagt gatggtggat ggctgtgaag atcaggacca ggacaggtgc
     1321 ggagataatg acaacccaag caccctcacg aaacttcgtg aaggtgaaga ccaacgtcac
     1381 tacggcagtg caaacggcgc cgaagccgtt gatcagcatt ttgagctgcc aatttggcgt
     1441 gtagctcaac gtcgagccgc gctcttggac cacttgcccc gattgcaggt ggccgatctt
     1501 ccaccagcgc cgcgccatgc cggcctgcga cagcgtgaac gagacgaaca ctccaatggc
     1561 atacagcggg atcagtgcag tcactctcgc ctggaagatg aggatcagca aggatgccag
     1621 tagagccagc gcgacgatcc cgcgcgagaa caccagtcgg ctgccgcgat aggtgaactg
     1681 gcggggtagg aagccatctc cggccacgag ggcgctcagg cgggggaagt cggcgaagga
     1741 ggtattggca gccataatca ggatgatcgt cgtcgcagcg atcagcatga ggtacagggt
     1801 gccccggctg tcaaagattg tccttgccag ttgggagatc acggtctctc cctcggaggg
     1861 gagagcatgg atttcgccgg ccaggaaggt gatcccaagg aacagcgtcc ccaagatgac
     1921 cgacatccag atcagggtga ttgccgcgtt gcggctgcgg ggctccttga aggcggggat
     1981 gccgttggaa atcgcctcga cccccgtgag ggcggtggtc ccacttgaga aggcatgcag
     2041 gatcaggaac ggcgtgaccg ctagaagagt ctccccaaat tccagcgccg gggggtcgat
     2101 gaccgaaccc aatgtgccgg tcacggcacg caccaggccg gttccgagcg tcagcaccat
     2161 catgagcaag aagaagtagg tgggaatggc aaaagctcgt ccggattccc gtacgcccct
     2221 caggttgacg atcatgatga tggtgaccat gccaaccgcg atcgcgaccc ggttggtgga
     2281 cagcgagggg tacgcagata cgatctgagc cacgcccgag gacaccgaaa ccgcgacggt
     2341 gaggacatag tcggtgagca gcgccgcgcc ggcagtctgc gccgggagct cgcccaggtt
     2401 gtcacgggcg acgatgtacg cgccgccgcc ccccgggtag gcgtggatcg tctgctgata
     2461 ggaaatggtc acgatcgcca gcaagccgac gatgcccatg gcgatgggga gggcatagcc
     2521 cagggcggcc gtgccggcca ccaccaggat gaccaggatc tcctgagtcg cataggccgt
     2581 agacgacagc gcgtcggagg cgaagaccgc caggccgatg gccttgccga tcgtctggtg
     2641 gggcgcg
//
LOCUS       DATDPQ010000155         5905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_326306, whole genome shotgun sequence.
ACCESSION   DATDPQ010000155 DATDPQ010000000
VERSION     DATDPQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326306"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VI520_03640"
     CDS             complement(<1..237)
                     /locus_tag="VI520_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HLF37009.1"
                     /translation="MGDTKREVVIPAGGAKPMGPYAPGIRTEGFVFASGSIGLDPTTG
                     TLVAGGIEAQTRQAIQNLSRVLEAGGSSLKQVVKT"
     gene            complement(293..2881)
                     /locus_tag="VI520_03645"
     CDS             complement(293..2881)
                     /locus_tag="VI520_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37010.1"
                     /translation="MAAAEAQPSPRALPTPALPDLVVNSVSANWGIVDDCVSFDSAFL
                     SVDIEIANIGSGRAGFFGVAVGPYPSEFIDEGLEAGESLLVHVSEFVTEDEGVRFDQQ
                     LGIATVDYLDQVQESNESNNSGRVQPELPIGIEHCATPEPVLAADLRPIPLYPQGQPL
                     SFQRVDMLDSSRGWGIVGRGYEDQHFVRTEDGGKTWFDVSPPELAYGVQECGAQAILR
                     AIDGSTAWVGYINAYYDPSDPYCLQVPVTDLWKTEDGGSTWRLSSFVQRNRDAIGGSA
                     IDGSGFPLLEFVDSQYGWALRDFFLGASSSAVELYRTIDGGQTWELMPEGYLHQFTGI
                     DFLDRKHGWVTKSYPSGFSPQLSLAQTHDGGFSWEHSEFALPGDPENYYDCAVRSPSM
                     RSSTTGQVIMACSTNDLEPRWFVYETNDSGRTWQIQPIPEQADQFVSATVGWRREYAP
                     AEMQTDDPDTQTWDLYWTFDGGRSWSKFGTATLVWGGDFDFVNAQKGWAVAKLSETEN
                     VLIRTSDGGRSWQPMQPVTLESPILSGRGDPPRISMPAELRALSSQTVGDLELLQEIP
                     AAGITALAAYPPGDLLLTSHQDGTVTSWDLTGAHYPRAVRLHSDWIYDLAVAEKAGYF
                     ATASKDGRLSLWAFYGYTEFVTHSDLGGEIASVTATSDFVFLTLASGGQDGIVRIWKT
                     DVSGSFGDLYYPDAPPNELQGHAGWVWDVALSSDGRRLASASVDRSVRVWDVESGEPL
                     ATLLAHTATVGTLAFSPDGNRLASAAWDGSVVLWDTETWQPLRVSGEHDARVHAASFS
                     PDGSLFATSADNGSMILWSAERGEPLRTLQVGEGAVRAVYFAPDGQLLITASDDGFLR
                     FWGVRP"
     gene            complement(3022..5508)
                     /gene="leuS"
                     /locus_tag="VI520_03650"
     CDS             complement(3022..5508)
                     /gene="leuS"
                     /locus_tag="VI520_03650"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HLF37011.1"
                     /translation="MTEATRSGQVVAERYDPGAIERKWQARWERDGLYTARVDPKRQK
                     FYFLTMLPYPSGDLHIGHWYAMTPSDARARYLSRRGYNVMYPIGFDAFGLPAENAAID
                     HNTHPMKWTYANIDRMRGQLRSMGAMWDWSREAVSSDPEYYRWTQWFFLQLFKHGLAY
                     KKMAPVDWCPKDQTVLAREQVVGEDRVCERCGTPVVKKDLEQWFFRITKYADELLDFS
                     HLDWPEPVRLLQTNWIGRSEGANVNFRTEGEDEIPIFTTRPDTLWGATFMVLAPEHPL
                     LAVLTTAEQRHTVRKYVEKATRQTDIQRQAMDKEKTGEFTGGYAINPVNGERIPVWVA
                     DYVLMTYGTGAIMGVPAHDARDFEFARKHGIEVRPVIRPPDGGAPDGVTMKESLVIYG
                     DMINSGPLTGTPQARAFTATIEYLEKHKLGERAVGYRLRDWLISRQRYWGAPIPIIYC
                     PVHGQVPVPDDQLPVRLPEDVEWLPTGQSPLKLHPTWKQTTCPIGGEPAERDTDTMDT
                     FMCSSWYHLRYLSPDYSQAAFDPEQYDYWMPVDTYTGGAEHATMHLIYTRFFHKALRD
                     MGIVRGPEPMLQLRNQGHILGPDGFRMSKSRGNVIDPDEQVHEYGADSVRAYLMFGYR
                     WPEGGPWKADNIHGVVRWLNRLWTLVLEPAGAAAGEADPVGTRELIRKTHQAIQAVSE
                     DLENFEFNTVVSELMELTNAIGDAKQQGLAGSPEFDAATEALLSLMAPVAPHIAEELW
                     ERLGKPYSIHNQPWPGHDPELAKEELITLVVQVNGKVRDRISVPAGISEAEARERALA
                     SPAAQRYMEGKHAKDVIVVLGRLVNIVLDS"
     gene            complement(5826..5898)
                     /locus_tag="VI520_03655"
     tRNA            complement(5826..5898)
                     /locus_tag="VI520_03655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5863..5865),aa:Ala,seq:ggc)
BASE COUNT         1107 a         1937 c         1711 g         1150 t
ORIGIN      
        1 ggtcttcacc acctgcttga gcgagctccc gccggcctca agcacccggc ttaggttctg
       61 gatcgcttgc cgggtctggg cttcaatccc gcctgcgacc agcgtgccgg ttgtggggtc
      121 caacccgatc gatccagagg cgaagacgaa tccttccgtc cggattcccg gcgcatacgg
      181 tcccatcggc ttggcgcccc cggcaggaat cacaacctcg cgcttggtat cgcccattta
      241 ctccctccct ctgtcattct agccttctac caaaagcaac gtccgatccg gttcacggtc
      301 gcacccccca gaagcggagg aagccgtcgt ctgaggcagt gatcagcagt tgaccgtcgg
      361 gcgcgaagta cacggctcgt acggctccct cgcccacctg cagcgtccgc agcggctcgc
      421 ctctctccgc gctccacagg atcatgctcc cattgtccgc gcttgtggca aacagcgacc
      481 cgtcggggga aaagcttgcc gcgtggaccc gagcgtcgtg ctcgcccgat actcgcagcg
      541 gctgccaggt ttcggtgtcc cacaacacca ccgaaccatc ccaggccgcc gatgccagtc
      601 ggtttccatc gggcgagaat gcaagtgttc cgacggtcgc ggtgtgggcc aacagggtgg
      661 cgagcggttc gccgctctca acgtcccata ctcgaactga gcgatcaacc gaggcggaag
      721 ccaacctccg gccatctgat gatagagcga cgtcccacac ccagcccgcg tgcccctgca
      781 attcattcgg tggcgcatcc ggataataaa ggtcgccaaa actaccgctc acatccgttt
      841 tccaaatcct gacgattccg tcttgtcccc cagaggcaag tgtcaggaat acgaaatcgg
      901 atgtggctgt cacgcttgcg atctcacctc caagatctga atgagtaacg aactcggtgt
      961 aaccgtaaaa cgcccagagg ctcaaacgcc catccttcga ggccgtggcg aaataacctg
     1021 ctttctcggc gaccgcgagg tcatagatcc aatcggagtg aagtcggacg gccctcgggt
     1081 aatgtgcgcc ggtcaggtcc caagaggtaa ccgttccatc ctggtggctg gtgagcagta
     1141 ggtcacccgg ggggtaggcc gccagcgcgg ttatgccagc agcggggatc tcctgcagca
     1201 gctcaagatc ccccacggtc tgggaagaga gcgcccgcaa ctcggctggc atcgaaatgc
     1261 gcggaggatc gccgcgcccg ctcaggatgg ggctttccaa ggtcactggc tgcatcggct
     1321 gccaacttcg cccgccatcg gaagttcgaa tcagcacgtt ctcggtttca ctcagcttgg
     1381 ccacggccca gcctttctgg gcattgacga aatcgaagtc cccaccccag accagggttg
     1441 cggtcccgaa tttggaccag ctccgccccc cgtcaaatgt ccaatacaga tcccaggtct
     1501 gggtgtccgg atcgtcggtt tgcatctccg caggcgcata ttcgcgcctc cagccaaccg
     1561 tggcggatac gaactgatcc gcctgctctg ggatcggctg tatctgccag gtccggccgc
     1621 tgtcgttggt ctcgtacaca aaccaccgcg gctccaggtc gtttgtcgaa cacgccatga
     1681 ttacctgtcc cgttgttgag gaacgcatgg agggggaccg tacggcgcag tcgtagtagt
     1741 tctcagggtc tcctggcagc gcgaactcgc tatgctccca gctgaagcca ccatcatggg
     1801 tctgagccaa actcaattgg ggactgaaac ctgacgggta gcttttcgtc acccagccgt
     1861 gcttccgatc caggaagtct atcccggtga actggtgcag gtaaccctcc ggcatcagct
     1921 cccaggtctg accgccgtcg atcgtccgat aaagctcgac cgcggaggag cttgcgccga
     1981 gaaagaaatc cctcagagcc caaccatact gggagtcgac gaactcgagc agagggaagc
     2041 cagagccgtc tatcgcagaa ccgccgattg cgtccctgtt ccgttgcaca aacgagctca
     2101 gccgccatgt actgcccccg tcctccgtct tccagaggtc ggtcactgga acctgcaagc
     2161 agtacggatc cgaaggatca taataggcgt tgatgtatcc aacccaggca gtgctgccat
     2221 ctatcgcgcg taggatggcc tgggcgccgc actcctgaac cccgtaggcc aattcgggtg
     2281 gggagacatc gaaccaggtc ttccctccat cctctgtccg cacaaagtgc tggtcctcat
     2341 acccccgtcc aacgattccc cagccgcgac tggaatccaa catgtcgacc cgttgaaagc
     2401 tcaggggctg accctgaggg tagagtggga tgggtcgcag atccgctgcg agcaccggtt
     2461 cgggggtcgc gcagtgttcg attccgattg gaagctccgg ctgaacccgc ccggagttgt
     2521 tggactcgtt gctctcttgg acctggtcga ggtagtcaac cgtggcgatc cccagctgtt
     2581 ggtcaaatct gaccccttca tcttcggtca cgaattcgga gacatgcacc aacaacgact
     2641 cccctgcctc aaggccttca tcgatgaact ccgacggata cggaccaact gcgactccaa
     2701 agaaaccggc ccgccccgag ccgatgttcg ctatctcgat atccacgctg agaaacgcag
     2761 agtcaaaact gacgcagtca tcgacgatgc cccagttggc cgaaaccgag ttcacaacga
     2821 gatccggaag agccggcgtg ggcagtgccc gcggcgaggg ttgggcttcg gcggcggcca
     2881 ccggtggcgt tggctccgct gatggactcg ctcgaccccc gcagctggcc aaaaaggcta
     2941 ccgacaggaa cagtcctgtg tttgctgtcc agcgcaggga cctgggaaat ctggaggtaa
     3001 tggtgatcct cctcgaagac cctaagagtc gagcacgata ttcaccagtc ggccgaggac
     3061 cacgatcacg tccttcgcgt gcttgccctc catgtaacgc tgggcggctg ggctggcgag
     3121 ggcccgctcg cgggcctctg cctcggagat tccagccgga acggaaatgc gatcgcggac
     3181 cttgccgttc acctgaacga ccagggtgat cagctcttcc ttagccagct ccggatcgtg
     3241 ccccggccag ggttgattgt ggatcgagta gggcttgcct aaccgctccc acagttcttc
     3301 ggcgatgtga ggcgcgacgg gcgccatcag cgacagcagt gcctcagtcg ccgcatcgaa
     3361 ctcgggcgag ccggccagcc cctgctgctt ggcgtcgcca atcgcgttgg tcagttccat
     3421 cagctcggat acgaccgtgt tgaactcgaa gttctcgaga tcctccgaga cggcctggat
     3481 cgcttggtgg gtcttgcgga tcaattcccg ggttcccacc ggatccgctt cgcctgcggc
     3541 cgccccggcc ggttccagta ccaaggtcca caaccgattc agccagcgga ccaccccgtg
     3601 gatgttgtcc gccttccagg gtccgccttc cggccagcga tagccgaaca tcaagtaggc
     3661 tctgaccgaa tcggctccgt actcgtgcac ctgctcgtcc ggatcgatca cattgccgcg
     3721 ggacttgctc atccggaatc cgtcgggtcc caagatgtga ccctgattgc gcagctgaag
     3781 catcggttcg gggccccgga cgatacccat gtcgcgcagg gccttgtgga agaaccgggt
     3841 gtagatcagg tgcatcgtgg cgtgctcagc tcccccggta taggtgtcga ccggcatcca
     3901 gtagtcgtat tgctccggat cgaacgcagc ctgcgagtag tccggactca aatagcgcag
     3961 gtgataccag gaggagcaca tgaaggtgtc catggtatcg gtgtcgcgct cggccggctc
     4021 gccgccgatc ggacaggtcg tctgtttcca ggtaggatgc agcttgagcg gactctgccc
     4081 ggtcggcaac cactcgacat cctccggcag ccgcaccggc agctgatcgt ccgggaccgg
     4141 cacctggcca tgcaccgggc agtagataat cgggatcgga gcgccccagt accgctgacg
     4201 ggagatcaac caatcccgca gccggtagcc caccgcccgc tcgcccagct tgtgcttttc
     4261 caggtactcg atcgtagcgg tgaaggcccg tgcctgcggc gttccagtca acgggccgga
     4321 gttgatcatg tctccgtaga tgaccagaga ctccttcatc gtgactccgt cgggcgcccc
     4381 accatccggc ggccggatca ccggtcggac ctcgatcccg tgtttgcgag cgaactcaaa
     4441 atcgcgcgcg tcgtgagccg gcacacccat gatcgcgccg gttccgtagg tcatcaggac
     4501 gtagtcggcc acccagaccg gaatccgctc gccattcacc ggattgatgg catagccccc
     4561 ggtgaactca ccggtctttt ccttatccat cgcctggcgc tggatatcgg tttggcgggt
     4621 cgctttctcc acgtatttgc gtacggtgtg ccgctgctcc gcggtagtca ggaccgccag
     4681 caacggatgc tccggggcca gaaccatgaa ggtagcgccc cacaacgtat ctggccgggt
     4741 ggtgaagatt ggaatctcgt cttcgccctc ggtgcggaag ttgacgttgg cgccctcgct
     4801 tcgcccaatc caattggtct gcagcagccg aaccggctcc ggccaatcca aatgggaaaa
     4861 gtccagcagc tcgtcggcgt acttcgtaat ccggaaaaac cactgttcca ggtccttctt
     4921 caccactggg gtcccgcagc gttcgcaaac tcggtcctcg ccgaccacct gttcgcgggc
     4981 cagtaccgtc tggtccttgg ggcaccaatc caccggtgcc atcttcttgt acgccaggcc
     5041 atgcttgaag agctgcagga agaaccactg ggtccagcgg tagtactccg gatcgctgga
     5101 caccgcttcc ctcgaccaat cccacatcgc tcccatgctg cgcaactgtc cgcgcatccg
     5161 gtcgatgttg gcgtaggtcc acttcattgg atgggtgttg tgatcgatgg cggcgttctc
     5221 ggccggcaac ccgaaggcat cgaagccgat cggatacatc acgttgtagc ctctacggct
     5281 caggtagcgg gcgcgggcat cggagggcgt catggcgtac caatgcccga tgtgcaggtc
     5341 gccggacggg tagggaagca tggtcaggaa gtagaacttt tggcgcttgg gatcgactcg
     5401 agcagtgtaa agcccgtcgc gctcccagcg tgcctgccac ttccgctcga tcgcgcctgg
     5461 atcatagcgt tccgccacca cctgtccgct tctagttgcc tcggtcacct caacctcctt
     5521 acctgtctag tccggagcca gaaatgaaaa gccccctcgt cgattcaggg acgaaggggc
     5581 actccgcggt accaccctga ttgcccggat gaaccccggg ccactcatct gcgcgttaac
     5641 gggcgctccg cccccgacta ctagtcttcc ccgggggggt ctgtgccgac aaaggctcgg
     5701 cacaacaggc gagttccgcc ttctggatcg ggcgctccaa ggcgccgggc tgcgctctca
     5761 ctctccgcag ctcgctgctg gacggcgtac tactcctgtc ctgaccggct attcaattgt
     5821 tcaggtggac ccagtgggat tcgaacccac gaccttctca atgccattga gacgcgctcc
     5881 caactgcgct atgggcccaa gctga
//
LOCUS       DATDPQ010000156         5352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1640381, whole genome shotgun sequence.
ACCESSION   DATDPQ010000156 DATDPQ010000000
VERSION     DATDPQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640381"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VI520_03660"
     CDS             <1..249
                     /locus_tag="VI520_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37012.1"
                     /translation="GARAVRRVIFGGATEVALDSAGRINIPPFLRDYAGLDGEVYLVG
                     VGESFEIWRAEEWEKQLQSVTDPEANAQRFADFDLSTG"
     gene            253..1164
                     /gene="rsmH"
                     /locus_tag="VI520_03665"
     CDS             253..1164
                     /gene="rsmH"
                     /locus_tag="VI520_03665"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLF37013.1"
                     /translation="MDRLSSEPHTPVLYQQVLSALDPQPGCRIIDGTVGAGGHAEGIL
                     ERSAPSGELLGLDRDPVALPLALQRLERFGPRLHLRQGSFRELSAHAAAVGWQAVDGV
                     LLDLGLSSMQLADPARGFSFQSEGRLDMRFDPGQELDAAELVNRLPEPELASLIARYG
                     EDREARRIARAIVAARPLQTTTHLAQVVTRAVRSRGSRIHPATRTFQALRIEVNEELT
                     ALDEALPQAVELLRPGGRLVVIAFHSLEDRLVKRFCRDASRSSEERPARLRLIQRKPV
                     RPEPEELNRNPRARSARLRAAEKIVPA"
     gene            1211..1651
                     /locus_tag="VI520_03670"
     CDS             1211..1651
                     /locus_tag="VI520_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37014.1"
                     /translation="MPQAFKQAPWRLQIRATSRTALWIVGLFVLGGLYLAVNAKVARA
                     GRELLVLERQQELLARANAELNATYAGLTAPELMMARAAYFGFRPVKPEEVEYIRVEG
                     AISDPSFVAPRPPRSETNRFGMLSPSYTETLGERLAAFLRGLGE"
     gene            1648..3552
                     /locus_tag="VI520_03675"
     CDS             1648..3552
                     /locus_tag="VI520_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLF37015.1"
                     /translation="MKRFPGFKPRGPRTAGSNLPAGPLAASGKITAASFRGTGETAGE
                     PPASVSASRIRVVMFGMALLTAGLLVQLVRVQFGTYAPVFAARKNEGVTRLERLVPAR
                     GLIFDRNGRLLAGNTTSYVIEIETRQLTAASRLSIADVLSKTLVLPLEQLYSQLSVDW
                     VARGQYRIRLTRTDSAGEPLPITVDKFVASALNGFLADPLAPDLSGLALVPAPTRVYP
                     AGSLAGHVLGFVNQEGQGFFGVEGFYDEWLAGKPITIARPMIAPEARLQPDPPAGVNL
                     VLTIDTDIQQMVELELAKAIESSGSEHGEILVMDPRNGEILAMASWPILDPNAYEPWL
                     TVAGDEEPVIAPAVGAMYEPGSTFKVITMAAALDAGVVEPDTEFIDTGQIEVGGNTIR
                     NWDGDAWGPQSMLGCMQHSLNVCLAWVASDRLGASTLYNYLRAFGVGQLTGIDLAGEV
                     PGQLRTPRHPEWTESDLGTNAFGQGVSLTPIQLLVAVSAVANQGLMVQPHLVRQVIGP
                     NGAYSPQTTVIGRPISEETAADMTTMLSASLTGETAYASVPGYQIAGKTGTAQIPTEF
                     GYDSHWTIASFIGWGPIDDPSFVVLVRLDKPEASPWGSVVAAPVFQEVARRLVVFLEI
                     APDALRMASG"
     gene            3549..5066
                     /gene="murF"
                     /locus_tag="VI520_03680"
     CDS             3549..5066
                     /gene="murF"
                     /locus_tag="VI520_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HLF37016.1"
                     /translation="MRLADASLELLPSFLTAVEPDSELAKILDRISRGPMLRLGTIIE
                     GITGRRLEGLDQVITDAATDSRLAISGSLFVALPGERSDGHDYVAQAFRRGAIAALVE
                     RPMGADFNQLDLRTEFDPAALRLPVCLRVENSLLALQAAAKHWRAQLPMRVIGITGSV
                     GKSTTKELTAEILQRRFRTHKNRGNLNNEIGLPISLLQASSAHDRAVLEMGFYVPGEI
                     ALLCEIARPQVGVVTNVSEVHLERAGSLEAIIKGKTELVENLPPAPEGVAILNIDDEA
                     VRGMAARTRARVFYYGLSPEADLWASDVEGLGLEGVRFRLHHLGETFHVRVPLLGQHS
                     VHTTLRAAAVGLTEGMSWDEIIAGLTQTHSQLRLVAVSGPGGSLLLDDTYNAAPPSVL
                     AALKLLEELDGRHVAVLGDMLELGEFEERGHRMVGARAAEVVDELVTVGERARWIGEE
                     ALRAGLPKSGLASVPDSKAAVEYLRGRIGSGDVVLIKGSRGIHMDQIVSELEVSD"
     gene            5063..>5352
                     /locus_tag="VI520_03685"
     CDS             5063..>5352
                     /locus_tag="VI520_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HLF37017.1"
                     /translation="MTQAAAALSLGGITFLLTVIWGGPLIQVLRLLKVGKSIRIEGPQ
                     RHFTKLGTPTMGGWLFIIPVLLITGVLNLVSILSELTVIGRSILLPMAIMLG"
BASE COUNT          866 a         1734 c         1822 g          930 t
ORIGIN      
        1 ggggcacgcg ccgtccggcg ggtgatcttt ggcggagcca ccgaggtcgc cctcgactcg
       61 gccgggcgaa tcaacatccc gcccttcctg cgcgactacg ccgggttaga cggggaggtc
      121 tacctggtgg gggtcggcga gtccttcgag atttggcggg ccgaggagtg ggagaagcaa
      181 ttgcagtcgg tgaccgatcc cgaggcgaat gcccagcggt tcgccgactt cgatttgtcg
      241 acggggtagg cggtggatcg gctgtcgtcc gagccgcaca ccccggttct ttaccaacaa
      301 gtgctcagcg ccctcgaccc gcaacccggg tgtcggatca tcgacggcac cgttggagcg
      361 ggggggcatg ccgagggaat tctggagcgc tccgcgccgt ccggggagct gttgggcctt
      421 gaccgggatc cggtcgcact gccgctggcc ctgcaacgcc tggaacggtt cggaccgcgc
      481 ctccacctgc ggcaaggctc gttccgagaa ttgagcgccc acgctgcggc ggtcggctgg
      541 caagcggtgg acggagtcct gctcgacttg ggcctgtcct ccatgcagct agccgacccc
      601 gcccgagggt tctctttcca atccgagggg cggctggaca tgcgcttcga cccgggccag
      661 gaactcgacg cggccgagct ggtcaatcgg ctgccggagc cggagctggc gagcctgatc
      721 gcccgctacg gagaggaccg cgaggctcgg cggatcgccc gagcgatcgt ggcggcccgg
      781 ccgctgcaga ccaccaccca cttggcccag gtggtcaccc gggcggtccg cagccggggc
      841 agccggatcc atccggccac ccgcacgttt caggcgctgc ggatcgaggt caatgaggag
      901 ttgaccgccc tggacgaagc gctgccgcag gcagtggagc tgctgaggcc gggcgggcgg
      961 ttggtggtca ttgcgtttca ttcgctggaa gaccggctgg tgaagcggtt ctgccgggat
     1021 gccagtcgaa gttccgaaga gcggccggcc cggctgcggc taatccagcg caagccggtt
     1081 cgaccggagc cggaggagct aaaccggaac cctcgcgccc gcagtgcacg actgcgggcg
     1141 gccgagaaga tcgtaccggc ctgaggggtc caaggtggca tgaagcttgc agaacgttat
     1201 cggaagacac atgccccagg cattcaagca agccccatgg cggctccaga ttcgcgccac
     1261 gagccggacg gcgctctgga tcgtggggct gttcgtgttg ggcggcctgt acctggcagt
     1321 caacgccaaa gtggccaggg ccggccggga gctgctggtg cttgagcgac agcaagagct
     1381 cctggcgcgg gccaacgccg agttgaacgc cacctacgcc ggactcaccg ctccggagct
     1441 gatgatggct agggcggcct atttcggctt ccgcccggtc aagcccgagg aagtggaata
     1501 catccgggtc gagggcgcga tcagcgaccc gagcttcgtc gctccccgcc cgccccgatc
     1561 ggagaccaac cgcttcggga tgttgtcccc cagctacacc gaaaccctgg gtgagcggct
     1621 ggctgccttc cttcgaggat tgggcgagtg aagcgctttc ctgggttcaa accccgcggc
     1681 ccgcgcactg cgggctcgaa cctgcccgcc ggtccgctgg ctgccagcgg caagatcacc
     1741 gcggcctcct ttcggggaac cggcgagacc gccggggaac cgcccgcctc cgtctccgcc
     1801 agccggattc gggtggtgat gttcgggatg gccctgctca ccgcgggact gctggtccag
     1861 ctggttcggg tgcagttcgg cacctacgcc ccggtgttcg ccgcccgcaa gaacgaaggg
     1921 gtcactcggc tcgaacggct ggtgccggcc cgcgggttga tcttcgatcg caacggccgc
     1981 ctgctggccg gcaatacgac ttcctacgtc attgagatcg aaacccgcca gttgaccgcc
     2041 gcgagtcggc tctccatcgc cgacgtgctc tccaagacct tggtgctgcc gctcgagcag
     2101 ctctactccc agctcagcgt ggattgggtg gcccgcggcc agtaccggat tcggctgacc
     2161 cgcaccgaca gcgccggcga gccactgccg atcaccgtcg acaagtttgt cgccagcgcc
     2221 ctcaatggat tcctggccga tccgctcgcc cccgatctga gcgggctggc cctggtgccg
     2281 gcgccgaccc gggtctatcc ggccggcagc ctggccggac acgtgctggg attcgttaac
     2341 caggaaggcc aggggttctt cggagtcgaa ggattctatg acgagtggtt ggccggcaag
     2401 ccgatcacta ttgcccggcc gatgatcgcg ccggaagctc gcctgcagcc cgacccgccc
     2461 gccggggtca acctggtgct cacgatcgat accgacattc agcagatggt tgagctggag
     2521 ttggccaagg cgatcgagag ctcggggtcc gagcacggcg agattctggt catggatccc
     2581 aggaacggcg agatcctggc catggcctcc tggccgatcc tggatcccaa cgcctacgag
     2641 ccctggctga ccgtagccgg agatgaagag ccggtgatcg cgccggcggt cggcgccatg
     2701 tatgagcccg gttccacctt caaggtgatc accatggccg ccgccctcga cgccggagtc
     2761 gtcgagccgg acaccgagtt catcgacacc ggccagatcg aagtcggcgg caacacgatt
     2821 cgcaactggg acggcgatgc ctggggaccc cagagcatgc tgggctgcat gcagcactcg
     2881 ctcaacgtct gcctggcctg ggtcgcctcc gatcggcttg gagcctcgac cctctacaac
     2941 tacctgcggg ccttcggggt cgggcagctg accggcatcg acctggcggg tgaggtgcct
     3001 gggcagctgc gcaccccccg ccatcccgag tggaccgagt ctgatctggg gacgaacgct
     3061 tttggacagg gcgtgtcgct taccccgatc cagctcctgg tagcggtcag cgcggtggcg
     3121 aaccagggcc tgatggtcca gccccacctc gtccgccagg tgattggtcc gaacggtgcc
     3181 tattcgcctc aaaccaccgt gatcggccgc ccgatctccg aagagaccgc tgcggatatg
     3241 acgacaatgc tatccgccag cctgaccggc gagacggcct acgccagcgt tcccggctat
     3301 cagattgccg gcaagaccgg caccgcccag attcccaccg agtttggata tgactcgcac
     3361 tggacgatcg cttcatttat cgggtgggga ccgatcgacg acccgagctt tgtcgtattg
     3421 gtacggctcg acaagccgga agcttcgccc tggggttcgg tcgtcgccgc tccggtcttc
     3481 caggaggtcg cccggcggct ggtggtcttc ttggagatcg cgccggacgc cctgcggatg
     3541 gcgtctggat gaggctcgcc gatgcctccc tggagctgct tccgtccttc ctcaccgccg
     3601 tcgaaccgga cagcgagctg gctaagattc tggaccggat ctcccgagga ccgatgttga
     3661 ggctggggac gatcatcgaa gggattaccg gccgccggtt ggagggtctc gatcaggtca
     3721 ttaccgacgc ggccaccgac tcgcgcctgg cgatctccgg cagtttgttc gtggcgctgc
     3781 cgggcgagcg cagcgacggg cacgattacg tggcgcaggc ttttcgccgc ggtgcgatcg
     3841 cggccctggt cgagcgcccg atgggcgcgg atttcaacca gctggatctg cggaccgaat
     3901 tcgatccggc tgccctgaga ctgccggtct gtctgcgagt cgagaactcg ctgctcgccc
     3961 tgcaggccgc cgccaaacat tggcgggccc agcttccgat gcgagtcatc gggatcacgg
     4021 gcagcgtcgg caaatcgacc actaaagaac ttactgccga gatcctgcag cgccgattcc
     4081 gcacccacaa gaatcgtggc aatctgaaca acgagatcgg cttgccgatc agcctgctgc
     4141 aggccagctc cgcccacgat cgggccgtgc tcgagatggg tttctacgtc cccggagaaa
     4201 tcgccttgct gtgcgagatc gcccgtccgc aggtgggggt cgttaccaac gtcagtgaag
     4261 tgcacctgga gcgagccggg agcttggaag cgatcattaa aggaaagacc gagctggttg
     4321 aaaacctgcc gccggccccg gaaggggtcg ccattctgaa cattgacgac gaggcggtcc
     4381 ggggcatggc tgcccgcact cgggctcggg tgttctatta cgggctctcg cccgaagccg
     4441 atctgtgggc cagcgacgtg gagggcctcg gcctggaggg agtgcgcttc cggctgcacc
     4501 acctgggcga gacgttccac gtgcgggtgc cgctcctggg tcagcattcg gttcacacaa
     4561 ccctgcgggc cgcggcagtc ggactcaccg aaggcatgag ctgggacgag atcatcgccg
     4621 ggctcaccca gacccacagc caattgcggc tggtggcggt cagcgggccg ggtggttcgc
     4681 tgctgctcga cgatacctat aacgccgcgc caccctcggt cttggccgcc ctaaagctgc
     4741 tcgaagaact ggacggacgc cacgtcgcgg tgttgggcga catgctggag ctcggcgagt
     4801 tcgaagagcg cggccaccgg atggtcggtg ctcgggccgc ggaagtggtg gatgagttgg
     4861 tgacggtggg tgaacgagct cgctggattg gcgaagaggc gctgcgggcc gggctgccga
     4921 aatccggact ggcctcggtc cccgacagca aggccgcggt cgagtatctg cggggtcgga
     4981 tcggctccgg cgatgtggtc ctgatcaaag gctcccgcgg aattcacatg gaccagatcg
     5041 tcagcgagct ggaggtgtcc gattgacgca ggcggcggcc gccctctcgc tcggtgggat
     5101 cacgtttctg ctcacggtga tctggggcgg gccgctgatc caggtgctgc gtttgttgaa
     5161 ggtggggaaa tcgatccgga tcgaaggtcc ccagcggcat ttcaccaagc tcggcacgcc
     5221 caccatgggc ggctggctgt tcattatccc ggtgctgctg atcaccggcg tgctgaacct
     5281 ggtctcgatt ctcagcgagc tgaccgtcat cggccgctcc atcttgctgc cgatggccat
     5341 tatgttgggg ta
//
LOCUS       DATDPQ010000157         3112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1119116, whole genome shotgun sequence.
ACCESSION   DATDPQ010000157 DATDPQ010000000
VERSION     DATDPQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119116"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1523
                     /locus_tag="VI520_03690"
     CDS             72..1523
                     /locus_tag="VI520_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HLF37018.1"
                     /translation="MFEASRWTDDPPCGHLVAHPYQTHYCSYIRVPKLLTLQSPQTPH
                     PDETIALLIVQAIELWLQVILNDVKSALRGLASGVYQPTKLLHRAARLIRLLDLHTDV
                     AESLLLHDLGLLLPISGPLDDIRSAQLRELESVTEHLARLELAGDSTHADSAHGLVGR
                     FGRWSERYQRLLADLFERDRPQAPSFDEYLKLDELLKLQVGAMGDWAVVGEWPTRLLA
                     PEQLSPDELMFIVVHQVFELWFRALLHELDQVLAELGAAEPAIPEAARRMRRVVRIQE
                     LLAEMIHIPATIAAMDFLRFRQMARTVDGVAHGRGLSPASGTESYQFRELEVIAGLKH
                     SVAYAEFLGGNSDLHTRFLTPRLEALLKQPSLPEAFGTVLAKRGLVDVIEIFKPADAA
                     NPHADLAELADLLLEFDHFFQLWRFNHLTMVQSMIGRKSGTGFLGPEYLKETVGLGMQ
                     GEDDRLLRTPQIRPRFFEDLWEARTRMQVGEQE"
     gene            1553..2704
                     /locus_tag="VI520_03695"
     CDS             1553..2704
                     /locus_tag="VI520_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLF37019.1"
                     /translation="MNEIDWAAWREEFPGLRLSTYLNTVSLGQLSRRSRAAMNQFMDL
                     WTETGSAAWYAHWLKEQQAAREQFARLIGADPQEVAIMPNVSSALGAISSSLDFAKRS
                     QVVTTALDFPTLTYHWLAKKREGLEAVILASPDGIQVPLAAFERAVGGKTVLIATSRV
                     YFTTGFIQDVNALAELAHRNGALLLVDDYQATGQVPINVRQTPVDVLITGGLKWLLGG
                     PGIAYLYVRRELIQTLQPTMTGWFSNARQFDFDPHHLEFHEDARRFEAGTPSVASIYA
                     GKAGLEIINEIGAVNVRHRTAELREDLVGRLTDRGFRLRLPPDPDTRSGITIVELEDP
                     MAVTRALAERRIIVDKRPGAVRLSPYFYNTPEENELVVEALNEITRGDR"
     gene            complement(2714..>3112)
                     /locus_tag="VI520_03700"
     CDS             complement(2714..>3112)
                     /locus_tag="VI520_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLF37020.1"
                     /translation="GPLTLAGAPLAVLQDPGLLDHAGEWETSQLLHYRPELVDLTALP
                     SGSLPPAGQVAVLGDDPRKGSPEQGRAIVERALQSWEAWIERLLGGQALEALHDLYSA
                     RTASYQAYLDRYYQGSWEAAIEAWWKERTG"
BASE COUNT          578 a         1006 c          965 g          563 t
ORIGIN      
        1 tgcttgacac gctaactcgc caacgagtac agtagcgcac tgagtccatc tcgctcaggg
       61 ccaacccaag aatgttcgaa gcatcccgct ggaccgacga cccgccctgc ggccacctgg
      121 tcgcccaccc ctaccaaacg cattactgca gctacattcg ggttcctaaa ctactgacgt
      181 tgcaaagccc tcaaacaccg catcccgacg agactattgc cctgctcatc gtgcaagcga
      241 tcgagctctg gcttcaagtg atcctgaacg atgtcaagtc ggccctccgg ggcctggcct
      301 ccggggtcta tcaaccgacc aaactgctgc accgggctgc ccgattgatt cgcctgctgg
      361 acctgcacac cgacgtcgcc gaatccctac tgctgcacga tctcggcctg ctcctgccga
      421 tttccggacc gctggatgac attcggtcgg ctcaattgcg cgagctagaa tcagttaccg
      481 agcatctggc gcgccttgag ctggcgggcg attccaccca cgccgattcg gcccatgggc
      541 tggtcggacg cttcggccgg tggagcgagc gctaccagcg actgctggcg gatctcttcg
      601 aacgggatcg gccgcaggcc ccaagcttcg atgagtatct taagctcgac gaattgctca
      661 agctgcaagt tggagcgatg ggggactggg ctgtggtcgg tgagtggccg acccggctgc
      721 tcgctccgga gcagctctcg cccgacgagt tgatgttcat cgttgtccat caagtcttcg
      781 agttgtggtt ccgggccctg ctgcacgagc tcgaccaggt gctggccgag ctcggcgccg
      841 ccgagccggc gatccccgag gccgcccgcc ggatgcggcg ggtcgtgcgg atccaggagc
      901 tgctggcgga gatgattcac atcccggcca ccattgccgc gatggatttc ctgcgtttcc
      961 gccagatggc aaggacggta gatggtgtcg cgcacgggcg gggcctctcc ccggcctccg
     1021 gcaccgagtc ttaccagttc cgcgaactgg aagtgatcgc gggcctcaag catagcgtgg
     1081 cctacgctga attcctcggc ggcaactcgg acctgcacac tcgcttcctg accccccggc
     1141 ttgaagctct gctcaaacag ccgagcctgc cggaggcttt cgggactgtg ctggccaaac
     1201 gtggactggt ggatgtcatc gaaatcttca agccggcgga tgccgccaac ccgcacgcgg
     1261 acctggccga gttggcggat ctgctgctcg agttcgacca cttcttccag ctctggcgct
     1321 tcaatcacct gacgatggtg cagtccatga ttgggcgcaa atccgggacc gggttcctgg
     1381 gaccggagta tctcaaggag accgtcggac tcgggatgca gggcgaggat gatcgattgc
     1441 tgcgcacgcc gcagatccgg ccgcgattct tcgaggacct ttgggaggct cgaactcgga
     1501 tgcaggtcgg agagcaggaa tagctgcaat tgcgaagagg ccggtcaaca ccatgaacga
     1561 gatcgactgg gccgcctggc gcgaggaatt ccccggcttg cggctgagca cttatctcaa
     1621 cacggtgtcg ctcggccagc tttcccgccg ttctcgggcg gcgatgaatc agttcatgga
     1681 cctgtggacc gaaacgggat ccgcagcctg gtacgctcac tggctcaaag agcagcaggc
     1741 tgcccgtgag cagttcgccc gcctgatcgg ggccgacccc caagaagtag caatcatgcc
     1801 caatgtttcc tccgcgctgg gggcgatcag ctccagcctg gacttcgcca agcggtctca
     1861 agtggtgaca accgccctcg atttcccaac cctgacctat cattggctgg caaagaaacg
     1921 cgaggggctt gaagcggtca tcctcgcctc gccggatgga attcaggtcc cgctggcggc
     1981 cttcgaaagg gccgttggcg gcaaaaccgt gctcatcgca accagccggg tctacttcac
     2041 caccgggttc atccaagatg tcaacgcgct ggcggagctc gcccatcgga atggggcgct
     2101 gctactggta gacgactacc aagccaccgg gcaggtcccg atcaacgtgc ggcaaacgcc
     2161 ggtggacgtg ttgatcacgg gcgggttgaa gtggctactc ggcgggccgg ggatcgccta
     2221 tttgtacgtc cgccgcgaac tgatccagac gctccagcca accatgaccg gctggttcag
     2281 taatgcccgc cagttcgatt ttgatccgca tcacttggaa ttccatgagg atgcccgccg
     2341 ctttgaggcc ggcacgccct cagtagcctc gatctacgcg ggcaaggccg gcttggagat
     2401 catcaatgaa attggggccg taaacgtgcg gcaccgcacc gcggagctga gagaagacct
     2461 cgtgggtcgc ctgaccgacc gcggttttcg actgcggctt ccgccggatc ccgacacacg
     2521 cagcggaatc acaatcgtcg agctcgagga tccgatggcg gtcacccggg ccctcgctga
     2581 acggcggatc atcgtcgaca agcgcccggg ggcagtgcga ctctcaccct atttctacaa
     2641 cacgccagaa gagaacgagc tggtcgtgga ggcgctaaat gagattaccc ggggcgacag
     2701 gtagccgatg gcctcagccg gtgcgctctt tccaccaggc ctcgatcgct gcctcccagg
     2761 agccctggta ataccggtcg aggtaggcct gataggaagc cgtccgggcc gaatagagat
     2821 cgtggagggc ctcgagcgcc tgccccccca ggagccgttc gatccaggct tcccaggact
     2881 ggagggcacg ctcgacgatc gcccggccct gctccgggct gcccttacgc ggatcgtcgc
     2941 caagcaccgc gacctggccg gccggcggga gcgaacccga gggcagcgca gtgagatcga
     3001 ccagctcggg tcgatagtgc agcagctggg aagtttccca ctctccggcg tggtccaaca
     3061 aaccggggtc ctgaagcaca gccaggggcg ctccggccag agtcaggggt cc
//
LOCUS       DATDPQ010000158         5456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_848282, whole genome shotgun sequence.
ACCESSION   DATDPQ010000158 DATDPQ010000000
VERSION     DATDPQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848282"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VI520_03705"
     CDS             <1..256
                     /locus_tag="VI520_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37021.1"
                     /translation="DLESLVNIDDSITVADLFIGENVRILTDLNELVARIIYEAEEII
                     PEAVVVAEAVEGVAELAEGAEAAAPEEAGKEEEEEGDERR"
     gene            complement(258..842)
                     /locus_tag="VI520_03710"
     CDS             complement(258..842)
                     /locus_tag="VI520_03710"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLF37022.1"
                     /translation="MSDDLAARLAGLLSQLPGGVQTEWEATPAAVLIPLFLDQGDWNL
                     LFTRRTDSVDVHRGQVAFPGGRIEPGDRSAAEAALRESEEEIGLRAADVRVLGQLNPL
                     LTVTQFAVTPVIGVIPWPYPLRPNHKEVAKCFGVPLHWLAESANLEVRSRQPLVPGLT
                     VQVYTFKPYEGEVIWGVTARITTSLLDLLAGSLA"
     gene            complement(844..1617)
                     /locus_tag="VI520_03715"
     CDS             complement(844..1617)
                     /locus_tag="VI520_03715"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HLF37023.1"
                     /translation="MGIKSQAGDLAMLVGKGRKQFLTQLNPENKLQTHWGVLRHADLI
                     EQDWGSRVKSHSGNEFLLFQPSLLDILLHTKRRSQIMYPKDIGYILLKLSVGPGSTVL
                     EAGTGSGALTSALAWAVGASGRVYSYDRRDDMQELARRNLERLGLLERVSLHLQDIEF
                     GLEPREVPSVFLDLPEPERYLTQVRQVMQSGGVLGSILPTTNQVSRLLAALDQHSFAL
                     VEVCEVLLRFYKTVADRFRPEDRMIGHTGYLVFARAMQG"
     gene            complement(1630..2301)
                     /locus_tag="VI520_03720"
     CDS             complement(1630..2301)
                     /locus_tag="VI520_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLF37024.1"
                     /translation="MKLLRAGLRLFFIHFYTSLAWTYDAVAYLVSVGQWNAWVLSAQE
                     AITADPVLEIGHGPGHLLVKLGPRATGLDPSRQMSRLAQRRLRRSGIRPRLVRACSQE
                     LPFRSACFGSLVSTFPAEYILEPRSIEEAWRVLRAGARLVIVAMAEIRGRGALDRLAN
                     WLFRITGQAGEILPEWTEALVQVGFEVHRVDVPVLRSNVVQIIGSKPSWPRAEPMDPV
                     PIQVI"
     gene            complement(2298..3659)
                     /gene="mgtE"
                     /locus_tag="VI520_03725"
     CDS             complement(2298..3659)
                     /gene="mgtE"
                     /locus_tag="VI520_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HLF37025.1"
                     /translation="MRPVDPSQIEHALERIRSALAAGHIQSAVDALRELHPADRAEAF
                     TDLEDDEVAALLPELSSRTTARLLTELGDRAAVDLAETLDTDRLADALDRMEPDEAAD
                     VLGDLDPRRVAEIVAEMEAAKGVLPLLGFPDNTAGGRMTTAFIAFPQSIPAADAIEML
                     RQAEPGSDTPYYLYVQNLAGRLVGVVGVRDLLVASPDASVESFMKSEVKSVRTNDDQE
                     VVARLMSRYGLGAIPVLDDQGVMRGVISHDDILDVIQQEATEDLYRLANLPSSDLSID
                     SPLVTSVRRRLPWLYVNAVTALFAAWVIGNFQTLLSQVAVLAVFQSVVAGMGGNTATQ
                     SLTLMVRAIALGDMEARKAKRTVLKEGLTGLLHGVFVGTAVGLGVWLWKGNQALGLIL
                     AVAIVGNMLVAGVVGALVPITLRSLKLDPALASSVIVTTFTDSIGFALFLGLGSVFLP
                     YLQ"
     gene            complement(3738..4442)
                     /locus_tag="VI520_03730"
     CDS             complement(3738..4442)
                     /locus_tag="VI520_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HLF37026.1"
                     /translation="MLLFSGDYLLFMLPAFILVMLAQWWVRSTYRKWGKVENGSGLTG
                     AEAAKRLLQYGGVGDVSLDSVPGQLTDHYDPRDHTLRLSAGVANGRSVASLAIAAHEI
                     GHAIQDKHGYAPLRFRAALVPIVNYGSRLGVFLILIGLVLRSVLGSQLAWVGVGMFAL
                     GAVFSLATLPVELNASARARALLSEAGILRSTEEEQGVRSVLTAAAFTYVAALAAAIL
                     QLMYWARIVGVGRRRN"
     gene            complement(4464..>5456)
                     /locus_tag="VI520_03735"
     CDS             complement(4464..>5456)
                     /locus_tag="VI520_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLF37027.1"
                     /translation="DDGSLVQGAIRGMVDSLGDPHSSYMDPDEYLQANLPLDGEYEGI
                     GAWVDADGEFLTIISAMPGSPAERGGLQPGDQVIGVDGEDVTGLDGNLVIRRVLGPAG
                     TAVRLTIRREGVPDPIDLELVRERIDLASVEGEMLEGDIGYIRLLSFGAQTADDLDRE
                     LADLLRQDPQGLILDLRGNGGGFLHTAVDVASQFLSEGVVLSERFGDGQETVYEVEGG
                     GRATQIALVVLIDGGSASASEIVAGAIQDHDRGLLVGETSFGKGSVQNWIELNDDQGA
                     VRVTIARWYTPDGRQIHEVGLTPDVEVLPAEAGQADLQLNEAIELLLGSAAQTG"
BASE COUNT         1096 a         1769 c         1669 g          922 t
ORIGIN      
        1 tgacctggag tcgttggtca acatcgatga ctcgatcacc gtggcggact tgttcatcgg
       61 tgaaaacgtc cggatcctga ccgatctcaa cgaattggtt gcccggatca tctacgaagc
      121 cgaagagatt atccctgagg ccgtggtggt ggcagaagct gtggaaggtg tggccgagct
      181 ggctgaaggg gctgaggcgg ccgcgcccga ggaggcgggc aaggaagaag aagaagaagg
      241 cgacgagcgg cgctgagcta ggccaagctg ccggcgagca agtccaacag actcgtggtg
      301 atacgggccg tcacgcccca aatcacttca ccttcgtacg gcttgaaggt atacacctgg
      361 actgtgagtc cgggaactaa tggctggcgc gagcggacct ccaggtttgc ggattcggcg
      421 agccagtgga gcggcacccc aaaacacttg gcaacttcct tgtgattcgg ccgcagcgga
      481 tacggccagg ggatcactcc gatcaccggg gtcaccgcga actgggtgac ggtgagcaag
      541 gggttgagct ggccgagcac ccggacatcc gcagcccgca acccgatttc ctcctctgat
      601 tctcgcagcg cggcctctgc cgccgaacgg tcgccaggtt caatccggcc gccgggaaat
      661 gccacctgcc cgcggtgcac gtccaccgag tcggttcgcc gggtgaacag caggttccaa
      721 tcgccctggt cgaggaagag cgggatgagc actgccgccg gagtggcctc ccactcggtc
      781 tgaacgcccc ctggaagttg ggagagcaat ccggccaacc gggcggccag gtcgtccgac
      841 actctagccc tgcatcgccc gtgcaaaaac caggtagccg gtgtgaccga tcatccggtc
      901 ttcaggccgg aaccgatcgg cgaccgtctt gtagaatcgc aacaggactt cgcaaacctc
      961 gaccagagcg aacgagtgct ggtcgagggc cgcgagcagc cgactaacct gattggtggt
     1021 cggaaggatg gagcccagta ctccgccact ctgcatcacc tgccggacct gagtcaagta
     1081 acgctccggc tcaggcaggt cgaggaacac ggacggaacc tctcgcggct caaggccgaa
     1141 ttcgatatct tgcagatgca ggctgacccg ttccagcagc ccgagtcgct ccaagttacg
     1201 gcgggcgagt tcctgcatgt cgtccctccg gtcgtaggaa tagactcgtc cgctcgcgcc
     1261 gaccgcccag gcgagggcgc tggtcaacgc tccggaaccg gttccggctt cgagcaccgt
     1321 gcttccggga cccaccgaca gtttcagcaa tatgtagccg atgtccttcg gatacatgat
     1381 ctgggaccgt cgcttggtgt gcagcaggat gtccagcaac gatggttgga acaacaggaa
     1441 ttcgttgccg ctgtggctct tcacccggga gccccaatct tgctcgatca aatcggcgtg
     1501 gcgcagcacc ccccaatggg tctgcagctt gttctccggg ttcagctggg tgaggaattg
     1561 tttgcggcct ttgcccacca gcatggccag gtcgcccgcc tgactcttga ttcccaagct
     1621 agcctcctct cagatcacct gaatcgggac cggatccatc ggttccgccc ggggccagga
     1681 tggtttggaa ccgataatct ggaccacgtt ggatcgcagc accggaacgt ccaccctgtg
     1741 gacctcaaac ccgacctgaa cgagcgcttc ggtccactcc ggcaggattt cgccagcctg
     1801 gccggtaatc cgaaatagcc agtttgccaa tcgatcgagc gccccccggc cacggatctc
     1861 ggccatggcg acgatcacca atctggcgcc cgcccgcagc actcgccagg cctcctcgat
     1921 cgatcgcggc tcaaggatgt attcagcagg gaacgtcgac accaaactgc caaagcatgc
     1981 cgatcggaac ggcagctctt gcgagcaagc ccgcacgagc cgaggcctga tcccactgcg
     2041 cctcaatctt cgttgcgcga ggcgggacat ctggcgggag gggtccaggc cggtggcccg
     2101 agggcccagt tttaccagca gatgaccggg tccatggccg atttcaagca ccggatcggc
     2161 ggtaatggcc tcctgggcgg aaagcaccca ggcgttccat tggccgacgg aaaccaggta
     2221 ggcgacagca tcataagtcc acgccaggct tgtatagaag tgaatgaaga acaagcggag
     2281 cccggcccgc agcagcttca ctgcaggtag ggaaggaata ccgagcccag gcccaagaat
     2341 agggcaaagc cgatgctgtc ggtgaaggtc gttacgatca ctgaggaggc tagcgcggga
     2401 tccagcttta aggacctcag ggtgatgggc acaagcgccc cgacgactcc cgccaccagc
     2461 atattgccca cgatggcgac tgccaggatc aagcccaacg cctggttccc tttccacagc
     2521 cacaccccca gacccactgc agtcccgacg aataccccgt gcagcaatcc agtcaagcct
     2581 tctttgagca ctgtgcgctt ggctttgcgt gcctccatgt caccgagcgc gatggctcgc
     2641 accattaggg tcaagctctg ggtggccgta ttgcctccca ttcccgccac cacagattga
     2701 aaaacagcca acacggcgac ctgggacagc aaggtctgga aattcccgat gacccaggcg
     2761 gcgaaaaggg cggtgaccgc gttaacgtac agccagggca atcgccgccg cacggaggtc
     2821 accagcggac tgtcgatcga gagatcggag gaggggaggt tggccagccg gtacaaatcc
     2881 tcggtggcct cctgctggat gacgtccagg atgtcatcgt gtgaaatgac gccccgcatc
     2941 accccctgat catcgagaac cggaattgcc cccaggccgt agcgggacat caagcgggcg
     3001 acgacttcct ggtcatcgtt ggtacgaacg gatttcacct cggacttcat gaagctctcg
     3061 accgaggcat ccggactggc cacgagcagg tcccgcaccc caaccacgcc gactaaccgg
     3121 ccggccaggt tttgaacata caagtagtat ggagtgtcgg atccaggttc tgcctggcgc
     3181 aacatctcga tggcgtcggc cgccgggatc gactgaggga aggcaatgaa ggcggtcgtc
     3241 atccgcccgc cggcggtatt gtcgggaaaa ccgagcaagg gtagtacgcc cttagccgcc
     3301 tccatctcgg ccacgatttc ggcgacgcgc cgtggatcga ggtcgcccag gacatccgca
     3361 gcttcgtcgg gctccatccg gtcgagggcg tcggccagcc gatcggtgtc gagagtctcg
     3421 gccagatcga cggccgcccg gtctcccagc tcggtgagca gtcgggcagt tgtccgggag
     3481 ctgagctcgg ggagcagcgc tgcgacttcg tcgtcctcca ggtcggtgaa ggcctccgcc
     3541 cgatccgccg gatgaagctc cctcaatgcg tcgaccgcag attggatatg tccagccgct
     3601 agcgcggatc gaatccgctc caaggcgtgt tcaatctgag aagggtccac gggtctcatt
     3661 gggttgggct gggaggcgga agggaagcgc gagcttctgc ggcttatggg gcgggcaggg
     3721 gccggcccgc ccttggctta gtttctccgc cggccgacgc cgacgatcct ggcccagtac
     3781 atcaattgca ggatggcggc ggccagggct gcaacgtagg tgaaggccgc cgcggtgagc
     3841 accgatctga ctccttgttc ctcttcagtg ctgcgcagga tcccggcctc ggaaagcagg
     3901 gcccgggcgc gggccgaagc gttgagctcc accggtaggg tcgccaacga gaacacggct
     3961 cccagggcga acattcccac cccgacccag gcgagctgag agccgagcac gctgcgcagc
     4021 accaaaccaa tcaagatcag gaacacacct agccgggacc cgtaattcac gattgggacg
     4081 agcgcggccc gaaaccggag cggagcatag ccgtgcttgt cttggatggc gtgaccgatt
     4141 tcgtgagcag caatggccag cgaggcgacc gaacgcccat tggcaacccc ggccgacagc
     4201 cggagagtgt ggtcgcgcgg atcatagtgg tcggtcagct gacctgggac gctgtccaaa
     4261 ctgacgtcac cgacccctcc atactgcagc agacgcttgg cggcttccgc gccagtcaac
     4321 cccgacccgt tttcgacttt gccccatttc cgataggtcg agcggaccca ccactgcgcc
     4381 aacatcacca ggatgaaggc cgggagcatg aagagcaggt aatccccaga gaacaacaac
     4441 atctcgcacc tcgcttctgc cgactacccg gtctgggcag cggaacccaa cagcagctcg
     4501 atggcctcgt tgagctgcag gtccgcctgc ccggcctccg ccgggagcac ttccacgtcc
     4561 ggagtcaggc cgacctcgtg gatttggcgc ccgtcgggag tataccagcg agcgatggtc
     4621 acccggaccg ccccctgatc gtcgttcagt tcaatccagt tctgaaccga gcccttgccg
     4681 aaggaagtct ccccaaccag cagccctcga tcatgatctt gaatggcccc ggcgacgatc
     4741 tcggaggccg aggccgatcc tccatcgatg aggaccacca gagcgatctg agttgcccga
     4801 ccgccgccct cgacctcgta gacagtctcc tgaccatcgc cgaatcgctc actgaggacg
     4861 accccctccg aaaggaactg ggaagcaaca tccacggcgg tgtgcaggaa gccgcctcca
     4921 ttgccccgca gatccaggat caggccttgc ggatcctggc ggagcaggtc ggctaattcg
     4981 cgatccaagt catcggccgt ctgcgctccg aaactcaaga gccggatgta tccgatgtct
     5041 ccctccaaca tctcgccttc gacgctcgcc aggtcgatcc gctcgcggac gagctccaga
     5101 tcgatcgggt ccggcacacc ctcccgccgg attgtcagcc ggacggcagt cccggcgggc
     5161 cccaagaccc tgcggatgac caggttgccg tcgagcccag taacatcctc cccgtcgacc
     5221 ccaatcacct ggtcgcccgg ctgaagcccg ccgcgttcgg ccggagagcc gggcatggcg
     5281 gagataatcg tgagaaattc gccgtccgca tcgacccagg cgccaatgcc ttcgtactcg
     5341 ccgtccaggg gaaggttggc ctgcaggtat tcatctggat ccatgtagga ggaatgcgga
     5401 tcgcccagcg aatcgaccat gcctcggatg gctccctgca ccagcgagcc atcgtc
//
LOCUS       DATDPQ010000159         7166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_66656, whole genome shotgun sequence.
ACCESSION   DATDPQ010000159 DATDPQ010000000
VERSION     DATDPQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66656"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VI520_03740"
     CDS             <1..239
                     /locus_tag="VI520_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37028.1"
                     /translation="RIDLLDALRLQPVAIRVAELAQGPLLTFRSLTLILVLSGAVLTT
                     LSVVLIAAAYNILARAGWGLSLELREPQRPGGSR"
     gene            322..1578
                     /locus_tag="VI520_03745"
     CDS             322..1578
                     /locus_tag="VI520_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLF37029.1"
                     /translation="MSEGTPEHMRERVDAIYHSDSGRILATLIRLLGDFDLAEEALQE
                     AFTAAMAQWPQSGVPANPRAWLVSTGRFTVIDELRQRVRFDASLAALADRFGPEANDA
                     RSPEEAEPVEDDRLRLIFTCCHLALPPEARAALTLREVCGLSTEEIARAFLIPPATLA
                     QRIVRAKAKIREARIPYEVPSRVELPDRLDSVLQVIYLVFNEGYSASSGASLTRADLS
                     SEAIRLGRLLVELLPEPEAIGLLALMLLHESRREARSSPAGDLILLEDQDRSLWNRDQ
                     ITEGIALVRAALASGRFGQYTLQATIAAVHAVASNFAATDWTQIVALYDRLAGMAPSP
                     VVELNRAVAVAMADGPAAGLALIDAILARGDLADYHLAHSAQAELRRRLGRTAEARAS
                     YERALALASQEPERRLLEQRLASLPS"
     gene            1702..2055
                     /locus_tag="VI520_03750"
     CDS             1702..2055
                     /locus_tag="VI520_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLF37030.1"
                     /translation="MQFLVYDPNYKPEAAPPPTPELMAEMGKFVAEAMQAGVVVATGA
                     LQAKGTRLRLSGGKFTVTDGPFIELKELLGGWAVLQVKSLDEAIEWSKRFRKIIGDGE
                     SEIVQVFGPDDFPPA"
     gene            2170..3285
                     /locus_tag="VI520_03755"
     CDS             2170..3285
                     /locus_tag="VI520_03755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HLF37031.1"
                     /translation="MSGVRVLVGTAKGAFVLASDGKRNKWDISGPHFGGLEIYHLKGS
                     PVAPDRIYASQATEWFGQVMHRSDDGGKTWETVGNEFRYEGETGPHQWYDGTPHPWEF
                     KRVWHLEPSLTDPDTVLAGAEDAAMFRSTDGGKTWQELPGLRSHPTGSKWAPGAGGLC
                     LHTILMDPSNPGRMYIAISSAGAFRTDDGGQSWRPINRGLKSEYIPDPEAEIGHCVHR
                     IAMHPSRPNVLFMQKHWDVMRSDDGGESWHEISGNLPSDFGFVIDVHAHEPETAYVIP
                     IKSDSQHFPPEAKLRVYRSRTGGNEWEALTNGLPQRDCYVNVLRDAMSVDKLDPCGVY
                     FGTTGGQVYASANAGDSWEPIVRDLPRVMSVEVQTLP"
     gene            3282..3578
                     /locus_tag="VI520_03760"
     CDS             3282..3578
                     /locus_tag="VI520_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HLF37032.1"
                     /translation="MIRVVLPYHLRNLAQVGKEISLGVEGQVTQHSVLDALELAYPML
                     RGTIRDHTTHERRPLLRFFACKEDLSFEPPDAPLPEAVASGAEPFLIIGAIAGG"
     gene            3680..4108
                     /locus_tag="VI520_03765"
     CDS             3680..4108
                     /locus_tag="VI520_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF37033.1"
                     /translation="MDFDSYRKTYFIDPAPDPRFSFADSFGVTIYFESFEAAIAYYEQ
                     VLGPPGYVEGEGTRGWRIGSGWLTLLRGKAGNPQNVEVAFEVETVDEAERLQRAFIAA
                     GGRGQAPSDQLMYRPIRSCPVVDPFGTEIMILSPLPFRGD"
     gene            4337..4753
                     /locus_tag="VI520_03770"
     CDS             4337..4753
                     /locus_tag="VI520_03770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HLF37034.1"
                     /translation="MIDFSTEFGRRVAKKLKSEQVIWLTTVGPGNTPQPRPVWFWWDG
                     KSVLIYSKPKGHKLRHIRRYPNVSLHFNSDAEANEVAVLVGRATVSRTAAPANDHKEY
                     VRKYRKGIADLGMTPKGFGEDYSVAIVVTPARLRGF"
     gene            5018..5461
                     /locus_tag="VI520_03775"
     CDS             5018..5461
                     /locus_tag="VI520_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HLF37035.1"
                     /translation="MIRIDDNLEAEITMPILTRVEASGASHVTLIGFDSPQTLRVVAS
                     GASSVGGEIQAGEMSIEASGASSISLAGGAANLVLEVSGASSVDLESFPVTDLRAELS
                     GASSAVVNASGTLDVEASGASELSYLGNPNLGSVNTSGASSVGEK"
     gene            5547..5963
                     /locus_tag="VI520_03780"
     CDS             5547..5963
                     /locus_tag="VI520_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37036.1"
                     /translation="MLGLLSAALAACAAGNASPTAAPMPLASPVSQEPAAGICGGTEA
                     AEVTITIYPDIPDPRCAIVRPEQRLRIVNSTVNPLQVSLASFQAEIGPGAEYAFDLPL
                     GEFLAPGVHLVQVLPCCGPELWLKPAQPVSGVPCRR"
     gene            6001..6465
                     /locus_tag="VI520_03785"
     CDS             6001..6465
                     /locus_tag="VI520_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37037.1"
                     /translation="MPVNVAVHSGDSHHSATPPDESALANGESIGPPPGIQISYHAES
                     IAFGTTRVLWWGGDWIEATTEAGFSAIGVQFWGALQGGWARILFEGVEVWRGLTSSLG
                     FEQLRFGGYVEISGFELQQHTIRTESLGFDYHPLTVAGFGFSREGGVASDSP"
     gene            6650..>7166
                     /locus_tag="VI520_03790"
     CDS             6650..>7166
                     /locus_tag="VI520_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37038.1"
                     /translation="MLVYDAERAPTNDPHEFLAFCGVVGLGKLLAEGKIRVLKDIRNR
                     ASDPRWRVREAVAMALQRWGRRDMPALLREMKGWSKGGRLEQRAAVAALCEPDLLGQK
                     VQVRKVLQILDTVTGSIAEARDRETEEFEALRKGLGYCWSVAVAAFPEQGRPRMERWI
                     GSPDPDVQWVMK"
BASE COUNT         1315 a         2210 c         2329 g         1312 t
ORIGIN      
        1 tgcggattga cctactggac gcactccgcc tgcagccggt ggctatccgg gtagccgaac
       61 tggcacaagg tccattgttg accttcaggt cactgaccct gatcctggtg ttgagcggtg
      121 cagtgctgac gaccctgagt gtggtgctca ttgcggccgc ctacaatatc ctcgcccggg
      181 caggttgggg cttgtccctt gagttgaggg aaccgcaacg gcccggcggg agcagataac
      241 cggcccaggc tccgcgatca gtcgagagtc acttgtaagg gcagccgggc cctccagcgt
      301 acgtcgaaaa atgtcgaagc ggtgagcgaa ggcacgcccg aacacatgcg cgagcgggtg
      361 gatgccattt accattccga ctcgggccgc atcttggcca ccctgatccg cctactgggc
      421 gacttcgacc tcgccgagga ggcgctccag gaggccttca ccgcggcaat ggcccaatgg
      481 ccgcaatctg gggtgccagc caatccccgg gcgtggcttg tttcgaccgg ccgcttcacg
      541 gtcatcgacg agctgcgcca gcgggtccgg ttcgacgcct cgctggcggc gctcgccgac
      601 cgattcggac ctgaggcgaa tgatgccagg agcccggagg aggctgaacc ggtcgaggat
      661 gaccggctgc ggctgatctt cacctgctgc catcttgccc tgccgccgga agctcgggcg
      721 gcgctcacgc tgcgcgaggt gtgcggcctc tcgaccgaag agatcgcccg cgccttcctc
      781 attccaccgg ccacgctggc ccagcgcatc gtgcgcgcca aggccaagat ccgggaggcg
      841 cgcattcctt atgaggtacc ctcgcgggta gagttgcccg accggctaga cagcgtgctt
      901 caggtgatct acctggtgtt caacgaaggg tattcggcct cgtccggggc gtcgctaacc
      961 cgggccgacc tctccagcga ggccatccga ttggggcggc tgttggtaga gttgttgccg
     1021 gagccagagg cgatcggact gcttgctctg atgctccttc acgaatcacg ccgggaggct
     1081 cgatcctcgc cagcggggga tctgatcttg ctggaagacc aggatcgctc gctctggaat
     1141 cgggaccaga tcacggaagg aattgcattg gtgcgggcgg cgctcgcctc cggccggttc
     1201 gggcagtaca ccctccaggc cactattgcg gcggtgcacg cggtcgcgtc caatttcgcc
     1261 gccacggact ggactcagat cgtggccttg tacgataggc tggcagggat ggccccctca
     1321 ccggtcgtcg agctgaaccg ggccgtggcg gtggcgatgg ccgacggccc cgcggcaggt
     1381 ctggctctga tcgacgccat cctggcccgc ggagatctgg ccgactacca cctagcgcac
     1441 tcggcgcagg cggaactgcg ccgccggctg ggtagaacgg ctgaggcccg agcctcctac
     1501 gaacgggcgc tcgcccttgc ctcccaggag ccggagcggc ggcttctcga gcagcggttg
     1561 gccagcctgc ccagctgaag ttgcatcagt ttgatgtcga tatggggcct ccccgcacga
     1621 ctatctattg aagcaaccta tccgccgagc ccggccaccc ttctaaggag gtccggctcc
     1681 caaccctaag gagagacgaa catgcaattc ctggtctatg atcccaacta caagccagag
     1741 gcggctcctc ccccgacccc ggagctcatg gccgagatgg ggaaattcgt agcggaggcg
     1801 atgcaggccg gcgtggtagt cgcgacgggg gcgctccagg cgaaggggac tcgcctccgt
     1861 ctctcgggcg ggaaattcac cgtgaccgat gggccgttca tcgagttgaa agagctcctg
     1921 ggcggctggg cggtgcttca ggtgaagtca ttggacgagg cgattgaatg gtctaagcgc
     1981 ttccggaaga tcatcgggga cggtgagtcc gaaatcgtgc aggtgtttgg gccggatgat
     2041 ttccctccgg cctgagaccg gatagcaata tcccggtcaa ccgagtcagc gctccttgtg
     2101 ggatactagg ctccgccttg ccatgggctc gtcggcgggc agcttcggac actcggtaag
     2161 gaggacgcga tgagcggagt acgtgttttg gtgggcaccg ctaaaggcgc attcgttctg
     2221 gcctcggacg gcaagcgcaa taaatgggac atcagcggcc cgcacttcgg gggcctggag
     2281 atctaccatc tgaaggggtc accggtcgcg ccggatcgga tctacgcctc tcaggccacc
     2341 gagtggttcg ggcaggtgat gcaccgttcc gacgacggtg gcaagacctg ggaaacggtc
     2401 ggcaacgaat tcagatacga gggcgaaacg ggaccccacc agtggtacga cgggacgcct
     2461 cacccgtggg agttcaagcg ggtctggcac ctcgaacctt cgctcaccga tccggatact
     2521 gtcctggcgg gtgcggagga cgccgcgatg tttcggtcga ccgacggagg caagacttgg
     2581 caggagctcc ctgggctgcg cagccacccc accggatcca agtgggcccc gggcgccggt
     2641 ggtttgtgcc tgcacacgat cctgatggac ccgagcaacc ccggacgcat gtacatcgcc
     2701 atctcgtccg cgggcgcctt ccgaaccgac gacggcggac agagctggcg accgattaac
     2761 cgcggactga agtccgaata catccccgac ccggaggccg agatcggcca ctgtgttcac
     2821 cgcatcgcga tgcacccatc ccgccccaat gtgctgttca tgcagaagca ctgggacgtg
     2881 atgcgcagcg atgacggcgg cgagagctgg catgagatca gcgggaacct gccgtccgac
     2941 ttcggcttcg tgatcgacgt ccacgcccac gagccggaaa ccgcctacgt cattccgata
     3001 aagagcgatt cgcagcactt cccgccggag gcaaaactgc gggtctaccg gagccgcacg
     3061 ggcggaaacg agtgggaagc actcaccaac ggcctcccgc agcgcgactg ctacgtcaac
     3121 gtcctgcggg acgctatgtc ggttgacaag ctcgatccgt gcggcgtgta cttcggcacc
     3181 accggcgggc aggtgtacgc gtcggcgaac gccggcgata gctgggagcc gatcgtgcgg
     3241 gacctgccgc gagtgatgtc ggtggaggtc cagaccctgc cgtgattcgg gtcgtcctcc
     3301 cgtaccacct gcggaacctg gcgcaggtcg ggaaagagat cagcctgggc gtcgagggtc
     3361 aggtcacgca gcattcggtg ctcgacgccc tcgagctcgc ctatccaatg ctgcgcggga
     3421 cgatccgcga ccataccacc cacgagcgcc gtcctttatt gcgcttcttc gcctgcaaag
     3481 aggatctttc gttcgagccg ccggacgccc cgcttcccga ggcggtggca tcgggggccg
     3541 agccctttct catcataggc gccattgccg gcggctagcc tggctggaac agaggcaacc
     3601 tctggtttcg cctgacatca agctcatgga gcggcccggc agcagaggtt gagagttagc
     3661 gagcgatcag ctggcagcaa tggacttcga ctcgtaccga aaaacctatt tcatcgatcc
     3721 ggcgcctgac ccgcggttta gctttgcgga ttccttcgga gttaccatct atttcgaaag
     3781 tttcgaggcc gcgattgcct actacgagca ggttctcggt ccgcctggat acgtggaagg
     3841 cgaagggacc cggggctggc ggatcggaag cggctggctg accctcttga gagggaaggc
     3901 aggaaatcca cagaatgttg aagtcgcctt cgaggttgag acggtcgatg aagcggaaag
     3961 gcttcagcgc gccttcattg cggctggggg ccgggggcag gctccctccg atcagctgat
     4021 gtaccggccg attcgatcct gcccggtggt tgacccattc ggcacagaga tcatgatcct
     4081 cagtcccctt cctttccgtg gagactaatc aggcatcgaa cctgctgacg gcccgcgtac
     4141 agcctgaact gcctcggctc taccgtgatt tggtcgaggg gtggccacga ccgtggccgc
     4201 cgggcattgc gatacctcga gtggagctgg gatcccgacc cggaggacac cacttacctg
     4261 accacaatgg tctacgcgct gacgcggctc ggttcaagcc tatgacactc atcgccgaca
     4321 acccggattg ggccccatga ttgacttttc tactgagttt ggccgtcgag tcgccaagaa
     4381 gctgaagtct gaacaggtca tttggctgac cacggttggc cccgggaaca ctccgcaacc
     4441 gaggccggtc tggttctggt gggatgggaa gtcggttctc atctatagca agccaaaggg
     4501 acataagctc cgccacatcc ggcggtaccc gaacgtttcg ctgcacttca actcagacgc
     4561 agaggcgaac gaggtggccg tgctggttgg ccgggctacc gtgtcccgga cggcggcgcc
     4621 agccaacgat cacaaggagt acgtccgaaa gtatcggaag ggtattgcag atctgggaat
     4681 gacacctaag ggttttgggg aggactattc cgtggcgatc gtggtgaccc ctgccaggtt
     4741 gcgcgggttc taaccccgca gccagcgatc cgctgcacgg agtcgcatac tggaagttcc
     4801 ctgatcaaga gagcatgtcg atcaacatca actctctcag gaagttcgtc ccgcttctct
     4861 ttaccctcta cccaattggg cttgtccgag cgagagcagt tttggaccgg atcgatactc
     4921 tacacgagaa acacgcggac gggccgcatt tctatctgga gaacctcggg gtgctgcctt
     4981 cgacgccacc gtccaacagg gcgagacctt cgcggtcgtg attcgaatcg acgacaatct
     5041 cgaagcggag atcaccatgc caatcctcac cagggtcgag gcgagcgggg caagccatgt
     5101 caccctgatc ggcttcgact caccgcagac ccttcgggtt gtagcctcgg gcgccagctc
     5161 ggttggggga gaaattcagg ccggtgagat gagcatcgaa gcctccgggg cgagctccat
     5221 ttcccttgcc ggcggggctg caaatctggt ccttgaggtc agtggggcaa gctcggtcga
     5281 tctcgaatcc ttcccggtca ctgaccttcg ggcagagctg agcggcgcaa gcagcgcggt
     5341 cgtgaatgcg agtggaacgt tagatgtcga ggccagcggt gcctccgagc tcagctacct
     5401 ggggaaccca aaccttggca gcgtgaacac ctctggcgcc tcgagcgtgg gagaaaagta
     5461 agccccaagt cagcgatcct gccttgaccc gaaacccaag ccggtcggcg ctccaatcct
     5521 tgccccgggg ggcagggagc cctgcgttgc tcggcctcct ttccgcggcg ctcgcagcct
     5581 gcgcggccgg caatgcctcc ccaacggctg cgccgatgcc gctcgccagc ccggtctctc
     5641 aggagccggc ggccggcatt tgcggaggta ccgaggcggc cgaggtcacg atcacgattt
     5701 accccgacat ccccgatccg cgctgcgcca tcgtccggcc ggaacagcgt ctgaggattg
     5761 tcaacagcac cgtcaatcca cttcaagtgt ctctggcctc gttccaggcg gaaattgggc
     5821 cgggagccga atacgcgttt gaccttccgc taggtgagtt cctggccccc ggggttcacc
     5881 tggtccaggt ccttccctgc tgcggccccg agttgtggct gaaaccagcc caaccagtat
     5941 ctggagtgcc ttgcaggaga tgaccccggc tgcgtatttt ttcccgttgc ccgagccagt
     6001 ttgccggtca acgtagccgt acattcgggt gactcccatc attctgcaac gccgcccgat
     6061 gaatctgcac tcgccaacgg tgagagcatc ggaccccctc caggaatcca gatcagctac
     6121 catgcggaga gcatcgcctt cgggacgact cgcgtgctgt ggtggggagg ggactggatc
     6181 gaagccacga ctgaggcggg attctctgcg ataggcgtac aattctgggg cgccctccag
     6241 ggtggatggg cccggattct tttcgagggg gtggaagtat ggcgcggact gacttccagc
     6301 ctcggattcg aacagcttcg attcggtgga tacgtcgaaa tctcgggatt tgaactccag
     6361 cagcacacga ttcgaaccga gagtctcggg tttgactatc atccgcttac ggtcgccggc
     6421 ttcgggttca gccgcgaggg gggcgtcgcg agcgactcgc cgtagaccct gcggatcagg
     6481 gatggcctga atcaagagca agaaggcaga tgcttatcgg gagacgctcc gccagctttc
     6541 ggactgggag cctttcttac tggccgagtc cggtctgccg gggccgaggg gaaaccttga
     6601 gctggcaaac gtcacggccg atgagggcga cgaacccttt tttcggcgga tgctcgtcta
     6661 cgacgcagaa cgagctccga cgaatgatcc ccacgagttc ctcgccttct gcggcgtggt
     6721 tgggttgggc aaactgctgg ccgaagggaa gatccgagtc ctgaaagaca ttcgcaatcg
     6781 ggcttcggac cccaggtggc gggtccgcga ggcggtcgcg atggccctcc agcgctgggg
     6841 gcggcgcgat atgcccgccc tgcttcgaga gatgaaggga tggagcaagg gaggccggct
     6901 cgaacaacgg gctgcggtcg ccgcgctctg tgagccggac ctgctcggtc agaaagtgca
     6961 ggtccgaaaa gtgcttcaaa tcctggacac ggtgacgggc tccattgccg aggcccgcga
     7021 ccgggagacg gaagaattcg aggcgcttcg caagggcctg ggctactgct ggagcgtcgc
     7081 ggtcgccgcc ttccccgagc agggaaggcc gcgaatggag cggtggattg gcagcccgga
     7141 tcctgacgtg cagtgggtga tgaagc
//
LOCUS       DATDPQ010000160         7328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1122120, whole genome shotgun sequence.
ACCESSION   DATDPQ010000160 DATDPQ010000000
VERSION     DATDPQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122120"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /gene="infB"
                     /locus_tag="VI520_03795"
     CDS             <1..1485
                     /gene="infB"
                     /locus_tag="VI520_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HLF37039.1"
                     /translation="VTILGHVDHGKTTLLDAIRQTEVAAGEAGGITQHIGAYQVSHND
                     RSITFLDTPGHAAFTAMRARGAQGADIAVLVVAADDGVMPQTREALAHARAARVPIVV
                     ALNKIDRPNANQDRVKQELADLGLVPDEWDGNTMVVPVSAKAVTGIEDLLEAILLVAD
                     STEIRSNPSGKVLGTVIEGQLDKAKGVIASLLVQNGTLRVGKIVIAGEAYGRVKAMFD
                     HLGKSIQQAPPSTPVSVLGLSAVPHAGDLFYVVDSEREAKALVKKLQESPTEVAAVGP
                     GLTLDQIFEAFKAGAAKELRLVVKADVQGSLEPIISSLNALSVGDIGINVLHWATGNI
                     GEDDVMLAAASEAIVIGFNVTPDAAAKRLAETRRVDIRVYDVIYRLTEDIEKALKGML
                     EPEKRTTEIGRAEVLQVFRIPKIGNIAGSRVTSGELRRNAKIRVVRGEEVLHEGTVSS
                     LKHEKDDVREVREGFECGVGLKGFDAFEQGDTLVAFTEEMVAVE"
     gene            1496..1849
                     /gene="rbfA"
                     /locus_tag="VI520_03800"
     CDS             1496..1849
                     /gene="rbfA"
                     /locus_tag="VI520_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HLF37040.1"
                     /translation="MPSEMRARRVADRIARELAILLQREVADPRLTGLTVTGVEVDRE
                     LAFATVYVTAQAPEQVLMPALVRARGFLRHELAARISMRAFPQLRFRWDDSLDRGARV
                     DELLARLKSETPDEE"
     gene            1839..2801
                     /locus_tag="VI520_03805"
     CDS             1839..2801
                     /locus_tag="VI520_03805"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HLF37041.1"
                     /translation="MRSEVVQAKQLLDGAARIAVVAHERPDGDAVGSMLGLCLSLRER
                     GKQTHAVLAGGLPGRFAFLPGADKVERRIPEGETLVVTVDAADLDRIGFPEVREVDIN
                     FDHHPSNTRFAAVNLVDPQAAATTSLIYHLAPELGLPITPAVAANLLTGLLTDTIGFR
                     TPNVTADTLRLAADLVDAGADLEDAYRKGLVERSFLAVRYWGQGLSKLERQGELVWAS
                     LSLADRKSVGYPGPDDADLIELLTAIQEARVSVVFVEQSENQVKVSWRGRPGVDVARL
                     AAQFGGGGHTLAAGAIIKGGLQPVMDQVLAATAAAIRPIEQTSA"
     gene            2798..3679
                     /gene="truB"
                     /locus_tag="VI520_03810"
     CDS             2798..3679
                     /gene="truB"
                     /locus_tag="VI520_03810"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HLF37042.1"
                     /translation="MSGFGVIVVDKPVGPTSHFVVDAVRRGTGVSKVGHAGTLDPRAS
                     GVLVLCLGPATRLSEYLSSSTKGYEAQVQFGKSTETYDGEGAAIKDTGKSPALEEILS
                     ALPGFRGDIQQVPPPYSAVKVSGKKAYQLAREGKPVELEPRTVTIFKLEPLEYQPPHL
                     TLWVECSAGTYIRSLAHDLGELLSCGAHLSQLRRVKAGPYTLNEAVGLPKLQRAMADG
                     SWPAYLRPAADALPDLPIVQLAPEELELVRNGLGVPTQTVPHGLARALAPNGELAAIL
                     EAIDGSQEWHPRKVFLA"
     gene            3680..4615
                     /gene="ribF"
                     /locus_tag="VI520_03815"
     CDS             3680..4615
                     /gene="ribF"
                     /locus_tag="VI520_03815"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HLF37043.1"
                     /translation="MQHVGDLAEVRLAASALTIGSFDGVHRGHQDLLGRMLAAAHGRG
                     LPAVALTFYPHPSVVLRGRKPAYYITSPEEKADLLAKLGIDLVITQTFDLELSRVEAP
                     DFLDRLTAQLGFQDLWAGEDFALGHQRRGDRHYLSRVSEERGFELHLVPPLMLEGEVV
                     SSTRVREALRSGDVARVAIYLGRLFELPGRVTRGVGRGRQLGIPTANLEVWEERAVPG
                     LGVYACLVDLDGRRKPAVVNIGVRPTFGESVSVPVVEAHLLDFEGDLYDRELKLVFVD
                     RLRDEQRFSGPDALIDQIGRDILRARELLGRVELA"
     gene            complement(4652..5614)
                     /locus_tag="VI520_03820"
     CDS             complement(4652..5614)
                     /locus_tag="VI520_03820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HLF37044.1"
                     /translation="MAERRTGTSPAGLSLAHSQPQALSLAARKRTHTSKYGSGGRSSH
                     DASAFYAGRLYSDRRPKPPTEATERSLPPELLDRLYLKTSEQMPELPDSSIHLMVTSP
                     PYNASKEYDKDLSLEEYRGFLKRVFSETYRVLVTGGRACVNLANLGRKPYIPLHAYLI
                     QDMLEIGFQMRGEIIWDKAASAAASTAWGSWRAATNPVLRDVHEYILIFSKEAFARKA
                     HGRESTIERDEFLEWTKSVWRIPAASARLVGHPAPFPLELPERLIKLYSYRGDVVLDP
                     FVGSGTTCVAALQNRRSYVGYETELAYLQLAEQRIRAAQAELEA"
     gene            complement(5545..6459)
                     /gene="era"
                     /locus_tag="VI520_03825"
     CDS             complement(5545..6459)
                     /gene="era"
                     /locus_tag="VI520_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLF37045.1"
                     /translation="MSSEHRSGYVAVAGRPNVGKSTLVNRYLGESILPVSPRPQTTHR
                     RQLGILTLPTAQVVFVDAPGMHSPRHRLGVVLDELAHDALRDADLVLCLFDLSRGPNP
                     DDRRVAGAVTSSQKPHLAAFNKIDLTPPAVLQANWEAYEQLLPAAPVYGVSATRGDNL
                     DQLLEGIVERLPAGPAYFPDAELTDSHERELAADLIRAAALQALRQEVPHSIAVLIDE
                     FRERHGRGAWIAATLFVERESQKGIVIGKGGHMLREIGTQARKDIERMSGRTIYLQLR
                     VKVLPKWRNDEQALRRLGYRSRTPSRRP"
     gene            6585..7208
                     /locus_tag="VI520_03830"
     CDS             6585..7208
                     /locus_tag="VI520_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HLF37046.1"
                     /translation="MGLFSRRKKPNRFIELLTKQAEHTSEGLQLLLQYVKNQDESLAR
                     QLTEFEKQADEVRRILIDELNRTFITPIDREDIFTLSLTIDDVLDYANSTLEEMAMFE
                     IKPTPFLERMVSLLSEAAREIHMGVMQLEDHPSVANDHAVRAKALENRMETVYREAIS
                     DLFHMPRDVDHIVEMLKLREVYRHLSNAADRGDAAANVIGDIVVKKM"
BASE COUNT         1369 a         2222 c         2379 g         1358 t
ORIGIN      
        1 gtgacgatcc tgggccatgt cgaccatgga aagaccaccc tgctagatgc aatccgccag
       61 accgaagtcg ccgcggggga ggccggaggc atcacccagc atatcggcgc ctaccaagtc
      121 agccacaacg atcgttcgat cacctttcta gacactcccg gtcatgccgc ctttactgcc
      181 atgcgggccc gcggggcgca gggagccgac attgcggtcc tggtggtagc cgccgacgac
      241 ggagtgatgc ctcagacccg ggaggcgctg gcccacgctc gggctgccag agtccccatc
      301 gtggtggcgt tgaacaagat cgatcggccg aacgccaacc aggacagggt gaaacaggag
      361 ctggcggacc tcgggctggt gccggatgaa tgggacggga acacgatggt cgttccggtt
      421 tcggcaaagg cggtcactgg gatcgaagat ctgctcgagg ccatcttgct ggttgctgat
      481 tcgaccgaaa tccgctccaa tccaagcggc aaggtattgg gaaccgtgat cgagggacaa
      541 ttagacaagg ccaagggtgt gatcgcgagc ctgttggtcc agaatggcac cctgcgggtg
      601 ggcaagatcg tgatcgccgg agaggcctat gggcgggtga aggcaatgtt cgaccatctt
      661 ggcaaatcga tccaacaagc tcctccctcg accccggtct cggtgctcgg actctcggct
      721 gtcccgcacg ccggagacct gttctatgtt gtggattcgg agcgggaggc caaggcgctg
      781 gtcaagaaac ttcaggagag cccgaccgag gtggctgccg tcggacccgg gctgactttg
      841 gaccagatct ttgaggcttt caaggcggga gccgccaagg aactgcggct ggtcgtcaag
      901 gcagatgttc agggctcact ggagccgatc atctcctccc tgaacgcatt gagtgtcggc
      961 gacattggga tcaacgtgct gcattgggcg accggaaaca tcggcgaaga cgacgtgatg
     1021 ctggcggccg cctctgaggc gattgtgatc ggcttcaatg ttactcccga cgcggcagcc
     1081 aagcgcctgg ccgaaacccg ccgagttgat attcgagtct acgacgtgat ctaccggctg
     1141 acggaggaca ttgaaaaagc gctgaagggc atgctcgagc cggagaagcg aacgaccgaa
     1201 attggaaggg ccgaagtgct gcaagtgttt cgcattccta agatcggcaa tattgccggc
     1261 tcccgagtga cctcggggga gctgcgccgc aacgccaaga tccgggtggt gcgaggcgag
     1321 gaggtcctgc acgaaggcac agtctcctca ctcaagcatg agaaggacga cgtgcgagag
     1381 gttcgagagg ggtttgaatg cggggtggga ctcaagggct tcgacgcctt cgagcagggc
     1441 gacaccctgg tggccttcac cgaggagatg gtcgccgtcg agtaggtctc cgaacgtgcc
     1501 aagcgagatg cgtgcccgca gggtcgccga ccggattgcc cgggagctgg cgatcctgct
     1561 tcaacgtgaa gtggcggatc cgcgactgac cgggctgacc gtgaccgggg tggaagtcga
     1621 tcgcgagttg gcattcgcca cggtttacgt cacggcccag gctcccgagc aggtgctgat
     1681 gccggcactc gtgcgggcgc gtggctttct gcggcacgag ctcgcggccc gtatttcgat
     1741 gcgggcgttc ccccagctca ggtttcgttg ggacgattca ctggatcgcg gcgcccgggt
     1801 ggatgagctg ctcgctcgat tgaaatcgga gacaccggat gaggagtgag gtcgttcagg
     1861 ccaaacagct gctcgacggg gcggcccgaa tcgcggtcgt ggcccacgaa cgacccgatg
     1921 gcgatgcagt gggttcgatg ctggggctct gcctcagcct gcgcgaacgg ggcaagcaga
     1981 cccacgccgt cctagcgggc ggcttgcccg gccgcttcgc attcttgccc ggggcggaca
     2041 aggtcgagcg ccggatcccc gagggcgaga ctctggtggt gacggtagat gccgccgatc
     2101 tggaccgcat cggattccct gaagttcgtg aggtcgatat caatttcgat catcacccta
     2161 gcaacacccg cttcgcggcg gtcaacctgg ttgaccctca ggccgcggcc acgacctccc
     2221 taatttatca cctggcacct gagctcggac tgccgatcac gcctgcagtc gctgccaact
     2281 tgctcaccgg gctgctcacc gatacgatcg gctttcgtac tcccaacgtc acggcggaca
     2341 ccctgcgcct ggcggccgat ctggtcgacg ccggggcgga tctcgaggac gcctatcgca
     2401 agggtttggt tgagcggagc tttctggcgg tccgctattg gggccagggc ctttcgaaat
     2461 tggagcggca aggcgagctg gtctgggcca gtttgagcct ggccgaccgg aagtcggtgg
     2521 gatatccggg cccggatgac gcagacctta tcgagctgct caccgccatc caggaagcac
     2581 gggtgagcgt tgtattcgtt gaacagtcgg agaaccaagt caaggtgagc tggcgcggcc
     2641 gtccgggagt cgatgtcgcc cggctggccg cccaattcgg gggaggcggc cacaccctgg
     2701 ccgccggcgc catcatcaag ggtggattgc agcctgtgat ggaccaggtg ctggcggcca
     2761 ccgcagccgc aatccgaccg atcgagcaga cgagcgcgtg agtggcttcg gggtaatcgt
     2821 cgtagacaag ccggtcggcc cgacctcgca ctttgtggtg gacgcggtcc gccgggggac
     2881 cggggtgtcg aaagtggggc acgccggcac cctcgatccg cgggcctccg gagtgctcgt
     2941 gctctgcctc ggcccggcga cccggctcag cgaatatctc tcgagttcga caaaaggcta
     3001 cgaggcccag gtccaatttg ggaaatcgac cgaaacctac gacggagagg gcgccgcgat
     3061 caaagacacc ggcaagtcgc ctgccctcga ggagatcctc agtgcgctgc cgggatttcg
     3121 cggagatatc cagcaggtcc cacctccgta tagcgcggtg aaagtgagcg gcaagaaggc
     3181 ctaccagctg gcccgagagg gaaagccggt ggagctggag ccccgcaccg tgaccatctt
     3241 caagctggag ccgctcgagt atcagccacc ccatctcaca ctgtgggtcg agtgctcagc
     3301 cggcacctac atccggtcgt tggcgcacga tctgggggaa cttctctcgt gtggcgcgca
     3361 cctttcgcag ctgcgtagag taaaggcggg cccctacacc ttgaatgagg cggttgggct
     3421 gcctaagctg caacgcgcga tggcggacgg ttcctggccg gcatacctcc gcccggcagc
     3481 cgatgcgctt cccgatcttc cgattgtcca attggcgccc gaggaactgg agttggtccg
     3541 gaatgggctg ggggttccaa cccaaaccgt tcctcatggt ctggcccggg ccctggcccc
     3601 aaatggggag cttgccgcca tcctagaggc catcgatggg agccaggagt ggcaccctcg
     3661 caaggtcttc ctggcctgaa tgcagcacgt cggggatctt gccgaggtcc gtctcgccgc
     3721 ctccgcgctg accatcggat ccttcgatgg cgtgcaccgc ggccatcagg atctgctggg
     3781 ccgcatgctg gcggccgccc acggccgcgg gcttccggca gtcgcgttga ccttctaccc
     3841 acatccctca gtggtgctgc gaggtcgcaa gccggcctac tacatcacct caccggaaga
     3901 gaaggccgat ctgctggcaa agctggggat cgatctcgtc atcacccaga cctttgacct
     3961 ggagttgtct cgggtcgaag ctcccgattt cctcgaccgg ttgaccgctc aactgggctt
     4021 ccaagacctg tgggccgggg aggactttgc cctgggccac caacggcggg gggaccggca
     4081 ctacctctct cgggtcagcg aggaaagggg cttcgagctt cacctcgtac cccctctcat
     4141 gctggagggt gaggtggtga gttcgactcg ggtgcgggaa gcgctgcgtt ccggcgatgt
     4201 ggcgagggtc gccatctacc tcggacgact cttcgaactg ccgggcaggg tgacccgcgg
     4261 agttgggcgc ggtaggcaac tagggatacc gacggccaac ctcgaggttt gggaggaacg
     4321 cgccgttccg ggactcggag tctatgcctg cctggtggac ttggatggtc ggcgaaagcc
     4381 ggccgtggtc aacattggcg tgcggccaac ctttggcgaa agcgtgagcg tgccggtggt
     4441 cgaggcccac ttgctcgatt tcgaagggga cctgtatgac cgagagctga agctggtctt
     4501 cgtcgaccgc ctgagggatg aacagcgatt ctccggcccc gacgcgctga tcgatcagat
     4561 cgggagagat attctgcgag cccgcgagct gctcgggcgg gtcgaactcg cctgagaatt
     4621 cgaccgctgg ccttcggtct gcgaattggg ctcaagcctc aagttcagcc tgggccgccc
     4681 gaatgcgttg ttcggcaagc tggaggtagg ccagctcggt ctcatagccc acataggacc
     4741 gtcggttttg cagcgccgcc acacaggtgg tccccgagcc gacgaacgga tccagcacca
     4801 cgtccccccg atagctgtat agtttgatca gccgctctgg aagctccagc ggaaagggcg
     4861 ccggatggcc caccagccga gcggaggcgg cgggaattcg ccagacgctc ttggtccatt
     4921 ccaagaactc atcccgctcg atcgtactct cccggccgtg cgcctttctg gcgaacgcct
     4981 cctttgagaa gatcagaatg tactcgtgga cgtcgcgcag gaccgggttg gtcgctgccc
     5041 gccacgatcc ccaggccgtg gaggctgccg cgctggcggc cttgtcccaa atgatctcgc
     5101 cccgcatctg gaatccgatc tccaacatgt cctgaattag gtaggcatgg agcgggatgt
     5161 acggcttgcg gccgaggttg gccaggttca cgcaggcccg ccctccggtg accagcaccc
     5221 ggtaggtctc cgaaaacacc cgcttcagga aaccccgata ttcctcgagc gacagatcct
     5281 tgtcgtattc cttgctggcg ttatacggcg gggaggtcac catcaggtga atgctgctgt
     5341 ccgggagctc cggcatctgc tccgaggtct tcaggtacag ccggtcgagc agttctggag
     5401 ggaggctccg ctcggtcgcc tcggtgggcg gcttcggtcg ccgatccgag tacagccggc
     5461 cggcatagaa cgcagaggca tcatggctcg agcggccgcc ggagccgtac ttgctagtat
     5521 gggttcgttt gcgagcggcc aaactcaggg cctgcggctg ggagtgcgcg agcgatagcc
     5581 cagccggcga agtgcctgtt cgtcgttccg ccactttggc agtactttga cccgcaactg
     5641 taaatagatg gttcgtccag acattcgttc gatatccttc cgcgcctggg tcccgatctc
     5701 ccgcagcatg tgtccaccct tcccgatcac gatccccttc tgcgattccc gttccacgaa
     5761 cagcgtggca gcaatccaag ccccacgccc atgccgttcc cggaattcat cgatcagaac
     5821 cgcgatgcta tggggcactt cctgccgcaa agcttgcaat gcagcggccc ggatcaggtc
     5881 ggccgccagc tcccgttcgt ggctgtcggt gagctcggca tctggaaagt aggccgggcc
     5941 agcgggcagc cgttcgacga ttccctccag gagttgatcg agattatctc cccgggtggc
     6001 cgagacgccg tagactggag cggctggcag cagctgctcg taggcctccc aattcgcctg
     6061 gaggaccgct ggcggggtca agtcgatctt gttaaaggcg gccaagtgcg gtttctggct
     6121 ggaagtcacc gcaccagcta cccgtcggtc gtcagggtta ggcccgcggc tcaaatcgaa
     6181 taggcacaaa accaggtctg catctcggag cgcatcatga gcgagctcgt ccaagaccac
     6241 tcccagccga tgccggggtg agtgcattcc aggggcatcg acgaacacta cctgtgcggt
     6301 cggcaaggtc aggattccaa gctggcgccg atgggtagtt tgcggccggg gtgaaaccgg
     6361 caggatgctc tcacccagat accggttcac cagagtcgat tttcccacgt tgggtcgtcc
     6421 cgccaccgcg acgtaaccgg aacgatgctc gctgctcact ccggaattct ccgagcaagt
     6481 cggcagggtg tcaagccgct gttctgctcg gccccaaccg ctataatagc ccgcgttctg
     6541 aagtcgaatc gaacacggcc gtgggggccg gaggctgaca aagcatggga ttgttctcta
     6601 gacggaagaa acccaatcgc ttcatcgagt tgttgaccaa gcaggccgaa cacacgtcgg
     6661 aaggcctgca gctcttgctc caatacgtga agaaccagga cgaatcgctg gcacgccagc
     6721 tcaccgaatt cgagaaacaa gccgatgaag tgcgccggat cttgatcgac gagctgaatc
     6781 ggaccttcat cacgccgatc gaccgggagg acatcttcac tctgtccttg acgatcgacg
     6841 atgtgttgga ttatgccaat tcaaccctcg aagagatggc gatgtttgaa atcaagccga
     6901 ccccgttcct cgagcggatg gtctcgcttc tcagcgaagc ggcccgggag atccacatgg
     6961 gcgtgatgca actggaagat catccgagcg tggcgaacga ccacgccgtg cgggccaagg
     7021 cgcttgagaa ccggatggag acggtctatc gggaggccat ctcagatctg ttccacatgc
     7081 cccgcgacgt cgatcacatt gtcgagatgc tcaagctgcg agaggtctat cgccacctat
     7141 ccaacgcggc agatcggggc gatgcggccg ctaatgtcat cggagatatc gtcgtaaaga
     7201 agatgtaggg ttcaggcggc cagccggtcg agcttgagac cgacctcgcg ccactgaatc
     7261 ttggaaacac cgagtttctg tcgcagattg tctttgtcga tcccggcctt gtaatcggtg
     7321 agggcggc
//
LOCUS       DATDPQ010000161         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1893327, whole genome shotgun sequence.
ACCESSION   DATDPQ010000161 DATDPQ010000000
VERSION     DATDPQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893327"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VI520_03835"
     CDS             <1..1095
                     /locus_tag="VI520_03835"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HLF37047.1"
                     /translation="PPGFTGWKGQLLSFIDPRFAEFLIDEAPSRIRVEEILWGGVTVD
                     GIPALDQAEVISAVAAGYLLPEEPVFGVSINGDARAYPLRILDWHEMANDVVGGVPMS
                     LAYCTLCGAAIAYDGRASDGETYTFGSSGFLFRSNKLMYDRQTRTLWNQLTGEPVLGE
                     LAATEVQLSLLPVVLTSWSAWQAQHPETLVLSLTTGFERYYLPGAAYGQYFASEATMF
                     PVWQRGSQLAAKDRIYALRVDGIPKAYPLARLVEEEVVNDSIGATNLVLIATRGRVAV
                     NGVSLQAGNVTYDAGGEVRAFLRGDEAFSPGTTPDTVVDERGLEWTVTEEGLRSPDGE
                     LAPRLSGHLSYWFGWFAFFPHTLIYLGASN"
     gene            1122..1712
                     /locus_tag="VI520_03840"
     CDS             1122..1712
                     /locus_tag="VI520_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HLF37048.1"
                     /translation="MDHRTRNYILIGLGLAVALPVGWYLLAPLFFDRVVQEDLPLAPA
                     EPTAEMKEAAEATAAMEEAMEEPDKLMEEPMPADEMADMRILAQGEFYNLAHEGQGRA
                     TIYELADSSRVLRFENFQVVNGPDLHVWLVPVDPVSDMVGKEIPGYVDLGTLKGNIGD
                     QNYDLPADLDLGGFKSVVIWCQPFRVPFNAAALRAP"
     gene            1746..>2192
                     /locus_tag="VI520_03845"
     CDS             1746..>2192
                     /locus_tag="VI520_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37049.1"
                     /translation="MSRPKQILVVEDEATIREAIRRYLEREGYQVREAADGYAALDIL
                     AEAEPDLIVMDLMLPGIDGLTLTRQLRQRSRVPVIMLTARGDTSDRIRGLDLGADDYL
                     PKPFSPRELVSRVQAVLRRGAPAAATRVRFSSVELDLGSRTVTVEAQ"
BASE COUNT          380 a          659 c          742 g          411 t
ORIGIN      
        1 ccgcccgggt tcactggatg gaaaggccag ctgctgagct ttatcgatcc ccgcttcgct
       61 gaattcctaa tcgatgaagc tccctcccgg atccgggtcg aggagatcct gtggggcggc
      121 gtgacagtgg acggcattcc agccctcgat caagccgagg tgatctccgc ggtggcggcc
      181 ggctacttgc tgccggaaga gccggtgttt ggggtctcga tcaacggcga cgcccgggcc
      241 tacccgctgc gcatcctcga ctggcatgag atggccaatg acgtggtcgg gggcgtgccg
      301 atgtcgctgg cctattgcac cttgtgcggg gcggccatcg cctacgatgg gcgagcctcc
      361 gacggggaga cctacacgtt tggctcctcc ggatttctgt tccgcagcaa caagctgatg
      421 tatgaccggc agactcgaac gctctggaat cagctcaccg gcgagccggt gctgggcgag
      481 ctcgcggcga ccgaagttca gctatcgctg ctgccggtgg tgctcacctc ctggtcggcc
      541 tggcaggccc agcatccgga gactttggtt ctgtcgctca cgaccggctt cgaacggtac
      601 tacctgccgg gcgcggcgta cggccagtac ttcgcctcgg aggcgaccat gtttccggtc
      661 tggcagcgcg gaagtcaact ggcggccaag gatcgcatct acgccttgcg ggtggatggc
      721 attcccaagg cctatccatt ggcacggctg gtggaggaag aagtggtcaa cgactcgatc
      781 ggcgccacga acctagtcct gatcgctacc cggggccggg tggcggtgaa cggggttagc
      841 ctgcaagccg ggaacgtgac ctacgatgca ggaggggagg tccgagcttt cctgcggggc
      901 gacgaggcct tctcgccggg gacaacgccg gatacggtcg tcgatgaacg gggcctggag
      961 tggacggtca cggaggaggg cctgcggtcg ccggatggcg agctggcacc gcggttgagc
     1021 ggccaccttt cctactggtt cggttggttc gccttttttc cacacaccct gatctatttg
     1081 ggagcgagca attgacccca tcttaaagga gagttcaaac catggaccat cgaacgcgta
     1141 actacatcct gatcggcctt ggactggcgg tggcgctgcc ggtgggttgg tatctgctcg
     1201 cgccgctctt tttcgaccgg gtagtgcagg aggatctccc gctggcccca gccgagccga
     1261 ctgccgagat gaaagaagcc gccgaagcga cggcggccat ggaagaggcg atggaagagc
     1321 cggacaagct gatggaagag ccgatgccgg ctgacgagat ggccgatatg cgcattctgg
     1381 cgcagggcga gttctacaac ctggcccacg aaggccaagg ccgcgcgacg atctatgagc
     1441 tggcggacag ctcgcgggtg ctgcggtttg agaatttcca ggtagtcaat ggacccgacc
     1501 tgcatgtctg gctagtaccg gtggacccgg tgtcagatat ggtcggcaag gagattcccg
     1561 gctacgttga cctggggacc ttgaagggca acatcggcga ccagaactac gacctgcccg
     1621 ccgatctcga tctgggcggt ttcaagtccg tcgtgatctg gtgccagcct tttcgggtgc
     1681 cgttcaatgc cgcggcccta cgcgcgccat gaatcgggac agatctgata cgatccgtcc
     1741 cggacatgag caggccgaaa caaatcctgg tcgtggaaga tgaggccacg attcgagagg
     1801 ccatccgccg ttatctggag cgggagggtt accaggtccg ggaggcggcc gatgggtacg
     1861 ccgccctcga tatcctcgcc gaggcggagc cggatctgat tgtcatggac ctgatgctcc
     1921 cggggattga cgggcttacc ctgactcgcc agctgcgtca gcgctcccga gtgccggtca
     1981 tcatgctgac cgcccggggc gacaccagcg accgaatccg tggattggat ttgggcgcgg
     2041 acgactacct gcccaagccg ttcagcccgc gcgagttggt ctcccgggtc caggcagtct
     2101 tgcggcgggg cgctcctgcc gccgcgaccc gggtccgctt cagttcggtc gagctggacc
     2161 tgggctcgcg cacggtcacc gtcgaagcac ag
//
LOCUS       DATDPQ010000162         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_69730, whole genome shotgun sequence.
ACCESSION   DATDPQ010000162 DATDPQ010000000
VERSION     DATDPQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69730"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VI520_03850"
     CDS             <1..223
                     /locus_tag="VI520_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HLF37050.1"
                     /translation="GLSVGWADVYDHYLPGQSIDISGLPDGIYALMSTADPYNLIQES
                     DETNNAAVVYLEIKGTQVSILDRAASPGS"
     gene            complement(303..1238)
                     /gene="lipA"
                     /locus_tag="VI520_03855"
     CDS             complement(303..1238)
                     /gene="lipA"
                     /locus_tag="VI520_03855"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HLF37051.1"
                     /translation="MLISADQIQELPSRPARRPEWIKVRAPAGPSYERVREIMRSKLL
                     HTVCEEARCPNLGECWGAGTATFLLMGDTCTRSCGFCDIATGRPQPLDWLEPERVARA
                     VKAMDLRHAVITSVNRDEREDGGAPIFAMVIRRIRELQPGCSVEVLIPDFKGSREALQ
                     IVMEARPEILNHNVETVRRLFKRVQPQDHYEWAQATLSNARQLDPDVLTKSGIMVGLG
                     ETLDELKETMRDLRSWGVDILTIGQYLQPSTKHLPIERYYHPDEFAELKDYGLGIGFR
                     WVESGPLVRSSYHAEQQVRALSVVHRELYGNERAS"
     gene            1855..>2091
                     /locus_tag="VI520_03860"
     CDS             1855..>2091
                     /locus_tag="VI520_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37052.1"
                     /translation="MPDTDQNFEGLGNVNNVLPPDPNGDVGPDHYVQTVNLSFAIWDK
                     SGQLLYGPANLNTLWQGFGGPCETTNNGDPIVLYD"
BASE COUNT          374 a          711 c          602 g          404 t
ORIGIN      
        1 ggggttgtcg gtcggctggg cggatgtcta cgaccactat ctgcccggcc agtcgatcga
       61 catcagcggc ctgcccgacg ggatctatgc cttgatgtcc accgccgatc cctacaacct
      121 gatccaggaa tcggacgaga cgaacaacgc ggcggtggtc tacttggaga tcaagggcac
      181 gcaggtctcg attctggatc gggctgcatc tccgggttct taacccgacg ggctgaatct
      241 aaccgactca aacaaaacgg cccggtagtc cgggcctttt catttgattc ggctctgccg
      301 acctagctgg cgcgttcgtt cccgtagagc tcccgatgca caactgagag cgctcggacc
      361 tgctgctcgg catggtacga ggagcgcacc agcggaccgc tctcgaccca gcggaagccg
      421 atccccagcc cataatcctt tagctcagcg aactcgtccg gatggtagta gcgctcgatc
      481 ggcaggtgct tggtcgaggg ctgtaggtac tgaccgatgg tcaggatatc cacgccccag
      541 gaacgcaggt cgcgcatcgt ctccttgagc tcgtccaggg tctcgcccag cccaaccatg
      601 atgccggact tggtcagcac atccggatcg agctgcctgg cattcgaaag cgtggcctgg
      661 gcccattcgt agtggtcctg cggctggacc cgtttgaaaa gccgccgcac ggtctcgacg
      721 ttgtggttga ggatctccgg gcgggcctcc atcacgatct ggagggcctc ccgcgatccc
      781 ttgaaatccg gaatcaacac ttccaccgag cagcccggct gcagttcgcg gattcggcgg
      841 atgaccatgg cgaagatcgg cgccccgcca tcctcccgct cgtctcggtt gacgctggtg
      901 atgaccgcgt ggcgcaggtc catcgccttc accgcccggg ccacccgctc cggttccagc
      961 cagtccagcg gctgcggccg gccggtcgca atatcgcaga agccgcagga gcgggtgcag
     1021 gtgtcgccca tcagcaagaa ggtggcggtt ccggcccccc agcactcgcc caggttcggg
     1081 cagcgggctt cctcacagac cgtgtgcagc agcttgctgc gcatgatctc ccgcacccgt
     1141 tcataactcg gtccggccgg ggcgcgtacc ttgatccact ccggacggcg cgccgggcgg
     1201 gaagggagtt cctgaatctg gtccgcagaa atcaacatcg actctggtga cgacctgcga
     1261 cagtcattca atcgattcta ccaagcttca gctctgcaca ttgccagcca gggtccggga
     1321 ggccgacttc tctcacgaga agtcacctgg catcccccaa aggtacggtt gtcgggtgca
     1381 ggcctcatca ttatccttcc cccttcaccc ttcccgtctt cgccacatca tcgcacggtt
     1441 caactccgcg gccaccaggg tctcgggccg ttatctaaca atgatgcgta cgcaaccgtc
     1501 gccttacttg tgcggactct tgagggttga cccgtggcgc ttccctagcg gtcaggagga
     1561 accctgatac gtagagcttt cgttccggca gcgtccctgg cgcttctggc tctcttggga
     1621 ccgccggtca ttgcccaatc tgcccgagtt gcccaaccgc ctccagaggc cacgccgacc
     1681 gttcaggagt cctcccggca tgacgcctcg ccgctgctgc gcaccatgcc gctaattccg
     1741 cccccggtcg gcgagctact agaactgccc cgcaggatgt tgccaggccg gcagggtagt
     1801 tcggggacat ccggcccgga tccagcgctc caaccaggcg gcggaacgcc ggctttgccc
     1861 gacaccgatc agaactttga gggcctcggc aacgtcaaca acgtgctccc gcccgacccc
     1921 aatggcgacg taggacccga ccactacgtg caaaccgtta acctgtcttt cgccatctgg
     1981 gataaatcgg gtcaattgct ctatggcccg gccaacctca ataccctctg gcaaggcttc
     2041 ggcgggccct gtgaaaccac taacaacggc gatcccattg tgctctatga t
//
LOCUS       DATDPQ010000163         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3742591, whole genome shotgun sequence.
ACCESSION   DATDPQ010000163 DATDPQ010000000
VERSION     DATDPQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3742591"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..366)
                     /locus_tag="VI520_03865"
     CDS             complement(157..366)
                     /locus_tag="VI520_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37053.1"
                     /translation="MSIVWNDEPHFFLASDDGMTVEVLLDEQLTAPFGGPLALDRTRV
                     VLLAVVTQASPAIFEALSIEREAER"
     regulatory      complement(722..811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1219..1929
                     /locus_tag="VI520_03870"
     CDS             1219..1929
                     /locus_tag="VI520_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37054.1"
                     /translation="MTVPTRVLVIDDDVTLANLLCMLLEKDGFGAIAATSGAEGLRLA
                     RQENPDGIILDVMMPGIHGYEVCRRLRKITDAAIVFVSVKGTTEDIVRGLHLGADDYV
                     VKPYTYEELSSRLLACLRRRNAGSLPPVYRASREVMLVTDPDRRLVFINNHETQLTPT
                     EFQVLKYLLKNQGKVLSTDAILANAWGPEYIGERELVKQFIYRLRAKLEQDPSTPKYI
                     QTVRGAGYVFEANGLLDD"
     gene            2288..2686
                     /locus_tag="VI520_03875"
     CDS             2288..2686
                     /locus_tag="VI520_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37055.1"
                     /translation="MSSTRVLMGVLLAALLLTLRPQAIGGAAAYLTVDEPGLEPALFP
                     GDLAIVRMQEDYRVGDLIAVQTLEGPFFGRIIGGDDTAYRVVFQGTADAVAVPGEYIV
                     GRLWFNLGVFGRRLSTGVLDAFGLEAEAAR"
     gene            complement(2683..>3354)
                     /locus_tag="VI520_03880"
     CDS             complement(2683..>3354)
                     /locus_tag="VI520_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HLF37056.1"
                     /translation="GIAFASIRATIGDSYTDDRFVQNWQGARAAGIFRAAYHVLRPDH
                     DPTSQIARFAEVVGEPGELPHVLDVELTGEQPDRVIRERTLACLQELEAHFGRKPLVY
                     TADWFWTPHIGVQPWVADYDLWVAHYYWPTVSEPKVPAGWSSWRFWQHSNRGQVVGVP
                     ARVDLDFFNGTTDELIAYAGGGTPPPPPPDVEQRLRNLERWAGELDTWARGQGYDGVR
                     PTGTG"
BASE COUNT          606 a         1045 c         1108 g          595 t
ORIGIN      
        1 ggggcggccg ccgcgctcaa ccagggtgtc gcccaactga caaacaacgc gaacaacagc
       61 agcgcgcgcc gcggcacgag gcgaatcgag cgggtcaaca tcgcacgccc acggccaggg
      121 cgggccctgg cgctgagcac attcatggag ccaatctcat ctctccgcct cgcgctcgat
      181 cgaaagcgcc tcgaaaatcg ccggcgatgc ttgggtgacc acagctagga gaaccactcg
      241 ggtgcggtcc agggcaagcg gaccgccaaa cggagcggtc aattgctcgt cgagaagtac
      301 ctcgacggtc attccgtcat cgctggcaag gaagaagtgg ggctcgtcgt tccagacgat
      361 gctcaaccag ccacgcaggg tgaccgttga ttgggaggtt ggtgctgaat gtggcttagt
      421 tggagttggg agcgttgcgg tccaagtgag gagcgggtcg gaagccgccg agggctcgcc
      481 tgggttaggg ccggaaggcg ccggcgatcc cgcggcgcag gcggatatga gcaggggacc
      541 caacgccagg ccgccgacag ggagcgccct gcgtattaag tgaagcggta gcctccacct
      601 cgcctgtgtc gccactctga ccccagtccc caggttccgg gcaaaacaaa agcccgggat
      661 gatcgccacg acggcggttc cacgggctgc gaaagactta ggctctcagt gcttgactca
      721 tttcggcagc tggctgtcgt ggctcgatgt gtgcgccgta gaccctgtag ctttgcgcca
      781 ccacctttcg gtggttttgc cttttcgttg agctggctat tacgccttag ttatcgttcg
      841 attgttaggt ccctgtaagg tgcttgccag cgtacggcca ctcagaggtg acggcaaccc
      901 tgacctgggt accaccgagg gaggtcctgg accttcaccc ggcgtttacg caatagtgac
      961 cctggtacgt taggatgaaa tcgcagggag ggggcttggc cggaggactc cgatcgtctc
     1021 accgcgctta tggctacagg tccgagttcg cgttgacact ccggattcgc cggtttagaa
     1081 tatcgctctg gaggcgaaga cccggggcct catttggtcg cttgccggcg agcacaaccg
     1141 gcagggcacc cggcgagcct agtagcgaaa ccggccattg ggccggtttg tcgtttgtgg
     1201 gatgagcagg ggtttgacat gacggtaccg acacgcgttc tggtcatcga cgatgacgtc
     1261 acgctagcca acctgctttg catgctgctg gaaaaggacg gctttggggc catcgcggca
     1321 accagtggcg cagaaggtct acggctggcg aggcaggaga accccgacgg gatcatcctc
     1381 gacgtcatga tgccgggcat ccacgggtat gaagtctgcc ggcggctgcg caagatcacc
     1441 gacgccgcca tcgtatttgt gagcgtgaag ggcaccaccg aagacatcgt ccgtggcctg
     1501 catctcggcg ccgacgatta cgtggtcaag ccctatacct acgaggagct gtcgtcgcgg
     1561 ctgctggctt gcctgcggcg ccgaaacgcc ggcagccttc cgccggtgta ccgcgcctcc
     1621 cgggaggtga tgctcgtcac cgatccagac cgacggctgg tcttcatcaa caaccacgaa
     1681 acccagctca ccccgaccga gtttcaagtg ctcaagtact tgctcaagaa ccagggcaag
     1741 gtgctcagca ccgacgcgat cctggccaac gcctgggggc ccgaatacat cggcgaacgt
     1801 gagctcgtga agcagttcat ctaccggctg cgggccaagc tcgagcaaga cccttccact
     1861 ccgaaataca tccagaccgt gcgcggcgcg ggttacgtgt tcgaagcgaa cggtctgctc
     1921 gacgactgag tcgtcgaaac cctcaccatt tgttgactcc ccagttacct cttattgacc
     1981 ttcccgcagt aagctaagtc cgccggaggg aggcgcggtg gggccgccaa tttggtcagt
     2041 ccccacccca tcgcgaccct cccttcctta caagcgggct cgagcgtccg tgccccgtcc
     2101 gagggaagcg aggccagacc tggggtcgaa ggttgcactt gggctgtcca cgggtttccg
     2161 cagggagggg cggggcgagc taagcgcccc gggtaggggg aacacgagaa accggcctcc
     2221 gtggccggtt tcttgcattc tacgcaagac gatctagatt ctgaatcccg cagggaggag
     2281 gagcacggtg agctcaacaa gggtgttgat gggggtgttg ctggccgcac tgctgctgac
     2341 gctgcggcca caggcgatcg gaggcgcggc cgcctatctc acggtcgatg agccgggtct
     2401 cgagccggcg ctgttcccgg gcgacctggc catcgtccgc atgcaggagg actaccgggt
     2461 cggggacctg atcgcggtcc agaccctcga aggaccgttc ttcggccgga tcatcggtgg
     2521 ggacgacaca gcctatcggg tcgttttcca ggggaccgcc gacgcggttg cggtgcccgg
     2581 cgagtacatc gtcgggaggc tgtggttcaa tctgggagtg ttcgggcgcc ggctcagcac
     2641 gggcgtgctc gacgcctttg gtctagaggc cgaggccgct cgctaaccgg tccccgtcgg
     2701 gcgcactccg tcgtatccct ggccgcgggc ccaggtgtcg agttctcccg cccagcgctc
     2761 caggttccgc agccgttgct cgacgtcagg cggtggcgga gggggcgtgc cgcccccggc
     2821 gtaggcgatc agctcatccg tcgttccatt gaagaagtcc aggtcgaccc gggctggaac
     2881 ccccacgacc tggccgcgat tgctgtgttg ccagaagcgc cagctcgacc aaccggccgg
     2941 gaccttcggc tcggagaccg tcggccagta gtagtgagcc acccataggt cgtaatcggc
     3001 cacccagggc tgcaccccga tgtggggagt ccaaaaccag tcggcggtgt agaccaacgg
     3061 cttacggccg aagtgggcct ccaactcctg caggcaggcg agggtccgtt cccggatcac
     3121 ccggtccggc tgctcgccgg tcagctcgac atccaacacg tgaggtaatt cgcccggctc
     3181 gccaacaacc tcagcaaagc gcgcaatctg agaggtcggg tcgtggtcgg gccgaaggac
     3241 gtggtaggca gcccggaaga ttccggccgc ccgggcgccc tgccagttct ggacgaaccg
     3301 gtcgtcggtg taggagtccc cgatggtggc ccggatcgag gcgaacgcga tccc
//
LOCUS       DATDPQ010000164         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1898202, whole genome shotgun sequence.
ACCESSION   DATDPQ010000164 DATDPQ010000000
VERSION     DATDPQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898202"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="VI520_03885"
     CDS             complement(<1..1769)
                     /locus_tag="VI520_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HLF37057.1"
                     /translation="MSHEPDEGSVDDEPAPKGRPGKKPAEGAPEPHTPSSIPQRHPTG
                     SPGLTGGWMAEADEPDLGDTQTSAQSAADSGSTPPSLGELPRRVPERDLSATQVTPAA
                     YVQVWEANLGQPPPRGGPGGCLLRMAILGLFAVIAVTIGVASFGLYQYYALASTLPPV
                     GDLQEHAAQFETTRILDREGNLLYEILDPQAGRRTYVPLEQISPAMVAAIISTEDSGF
                     YSNPGFDPAAIARAIWQNLQEGETVSGASTITQQIARNLLLSPEERTQRTALRKVREI
                     LLAAEIARRYTKDEILELYLNQVYYGNLAYGVEAASQTYFDTPAADLTLGQASFLAGL
                     VQAPAVYDVFVNREAALDRQRQVLVLMVKATSEQGCIYVSNSIERLCITPELAGAASA
                     ELVEYEFNSPDVAIRFPHWVNYVRAELEARFDPQTIYRSGFTVYTSLDPELQEAAEDI
                     VQKQVASLTEQHKVGNGALLALQPSTGEILVMVGSADFYAEAIDGQVNMILALRQPGS
                     SLKPLTYAAAFERGWTPGTLLWDVESEFPPYKDPEDPSKPYKPRNYDERFHGPVTVRA
                     ALANSYNIPALKTLEFVGIYDDPS"
     gene            complement(1828..>2047)
                     /locus_tag="VI520_03890"
     CDS             complement(1828..>2047)
                     /locus_tag="VI520_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37058.1"
                     /translation="EGTVLAWREISDWRFSQPVFIGDTVQVELEVSETKPMPRLNAGM
                     VGLAVQVNNQDGKAVMNGRWTLLVLNQP"
BASE COUNT          363 a          634 c          665 g          385 t
ORIGIN      
        1 gacggatcgt cgtaaatgcc aacaaactca agggtcttga gcgccgggat gttgtaggag
       61 ttggcgagcg ccgcccggac tgtgactggg ccgtggaagc gttcgtcata atttcggggc
      121 ttgtacggct tggacgggtc ctccggatcc ttgtagggcg ggaactcact ttccacgtcc
      181 cacaacaaag ttccgggcgt ccagccccgt tcgaaggcag cagcataggt cagtggcttc
      241 aacgaggacc cgggctggcg cagcgccagg atcatattca cctgcccatc gatcgcctcg
      301 gcatagaagt cggctgagcc gaccatgacc aggatctcac cggtgctggg ctgaagcgcc
      361 agcaaggctc cgttgccgac cttgtgctgc tcggtcagac tcgcgacttg tttctgaacg
      421 atgtcctcgg cggcctcttg cagctccggg tccaggctgg tgtagaccgt gaacccggac
      481 cggtagatcg tctggggatc gaagcgagcc tccaactcgg ccctcacgta attcacccaa
      541 tgcgggaacc ggatcgcgac gtccggggaa ttgaactcgt actcgaccag ctcggccgaa
      601 gccgcgccgg caagttctgg ggtgatgcac agccgctcga tcgagttgct gacatagata
      661 caaccctgct cgctggtggc cttcaccatc aaaaccaaga cctgtcgctg gcggtccaag
      721 gcagcttccc ggttgacaaa cacatcgtag accgcagggg cctggacgag cccggccaag
      781 aaagaggcct gaccgagggt caggtcggcg gccggagtat cgaagtaggt ctgagaggca
      841 gcctccaccc cataggccag gttgccgtag tagacctggt tcaggtagag ctcgaggatt
      901 tcgtccttgg tgtagcgccg ggcgatttcg gccgccagca ggatttcacg gaccttgcgc
      961 agtgcggttc gctgggtgcg ttcctccggc gaaaggagca agttgcgggc gatctgctgg
     1021 gtgatggtcg aggcgcctga aaccgtctct ccctcttgca agttctgcca gatcgcgcgg
     1081 gcgatcgcgg ccggatcgaa ccccggatta ctgtagaagc ctgaatcctc ggtagagatg
     1141 atggcggcca ccatggctgg ggagatctgt tccagcggca cataggtgcg gcgccccgcc
     1201 tgcgggtcca ggatttcgta gagcaaattg ccctcccggt ccagaatccg ggtcgtttcg
     1261 aattgggccg cgtgctcctg caaatccccg accgggggta gggtagaagc gagggcgtag
     1321 tactggtaca gaccgaagga ggcgactcca atcgtcactg cgatgaccgc gaacaggcca
     1381 aggatcgcca tccgcagcag gcatccaccc ggcccacccc tcggaggggg ttggccgagg
     1441 ttggcttccc agacctgcac ataggccgcc ggggtgacct gggtagccga aaggtcacgc
     1501 tccggcaccc gccgaggcag ttctccgagc gagggcgggg tcgatccgga atctgcggca
     1561 gattgggcgg aggtctgggt gtcgcccaga tccggctcgt cagcttccgc catccagcct
     1621 ccggtcaagc cgggcgaacc ggtggggtgg cgctggggga tggaactggg agtgtggggc
     1681 tcgggtgcgc cctcggcagg tttcttccct ggcctgccct tgggcgctgg ctcgtcgtct
     1741 accgagccct catccggctc gtgactcatg ggggcggatt atagcaccgg ctctgccgga
     1801 cctgctggcg gcctccggct gccggcttca cggctggttg aggaccagca gggtccaacg
     1861 cccgttcatc accgccttgc catcctgatt gttgacctgt accgcgagcc cgaccatccc
     1921 ggcgttcaac cggggcatcg gtttggtctc gctgacctcc aattccacct gaaccgtatc
     1981 gccgataaag accggctggc tgaagcgcca gtcactgatc tcccgccagg ccaatactgt
     2041 cccttcc
//
LOCUS       DATDPQ010000165         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2174195, whole genome shotgun sequence.
ACCESSION   DATDPQ010000165 DATDPQ010000000
VERSION     DATDPQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174195"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VI520_03895"
     CDS             complement(<1..1037)
                     /locus_tag="VI520_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HLF37059.1"
                     /translation="MTIHFGTDGWRGVIGDTFTFHNLRMVSQAIADGVASEDWVPRGQ
                     PAGDPRRMVVGFDTRFLSDRFAVEAARVLAGNGFTVSLTSADVPTPTASFAVRQLGAI
                     AGIMITASHNAPRYNGVKLKAGYGGSAAREQCKQVELCFQDNEARARGPNLMDYEQAK
                     RSGQIERFNPTPAYHDHLRTLIDLNVIADHPQHLVVDAMYGSGRGQLKGILQGTGCEV
                     LEVRGEMNPGFGGIHPEPIARYLGALVGAIAAGHGQLGLATDGDADRIGAVDGRGQFV
                     DPHRIMALALKYLVEQRGWRGTVVKTVSTTQMINRLAEKYSLPVIETPVGFDHIADRM
                     LQDEVLIGGEES"
     gene            1103..>2403
                     /gene="uvrC"
                     /locus_tag="VI520_03900"
     CDS             1103..>2403
                     /gene="uvrC"
                     /locus_tag="VI520_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HLF37060.1"
                     /translation="MPQPLLEGIPAKPGCYLMKDESGKVIYVGKAVNLRSRVRSYFHE
                     SGSADRKTAELVARVADVEWIVVASELEALILEMNLIKRHRPKYNVRLKDDKRYPYVR
                     VHWGDPFPKVTMTRQVVGDGSRYYGPYTSVWAVHQTLDVLRRIFPYLTCDRVITGQDL
                     RACLYHDIKLCLAPCIGAVDQAGYREMIGDLCGFLEGRTEPVIQRLQAEMARASGAME
                     YERAAAIRDQLTAIDRVIEGQKVVSRENLDSDVIAFARDDRSACVQVFFVRGGKLIGR
                     EYFVLEGTRQAGDEEVVRAFVKQFYANSSTVPQRVLLPLEIEEAAIIEAWLNRRSPEH
                     KVKITVPRKGVKRELVEMAAENAAETLSMLKARWEADRSKHVQALAELQAALQLGEPP
                     VRIEGYDISNLQGTAAAGSMVVFENGAPNKALYRKYTIKSV"
BASE COUNT          475 a          736 c          772 g          420 t
ORIGIN      
        1 gactcctcgc ctccgatcag cacctcatcc tggagcatcc gatcggcgat gtggtcaaac
       61 ccgaccgggg tctcgatgac cggcagcgaa tacttctcgg ccaggcgatt gatcatctgg
      121 gtggttgaaa ctgtcttgac caccgtaccc cgccagccgc gctgctcgac caggtatttc
      181 agagccaggg ccatgattcg gtggggatcc acgaactggc cgcgcccgtc cacggctccg
      241 atccggtccg cgtcgccgtc ggtcgccagc cctagctggc cgtgtccggc ggcgatcgct
      301 ccaaccaacg cgcctagata gcgggcgatc ggttccggat gaattccgcc aaaacccgga
      361 ttcatttcgc cccgcacttc aaggacctca cagccggtgc cctgcaggat gcccttgagc
      421 tgaccccgac ccgatccgta catggcgtcg acgacgaggt gctgtgggtg gtcggcgatc
      481 acattcaagt cgatcagcgt gcgcaggtgg tcgtgataag cgggagtggg attgaagcgc
      541 tcgatctgcc cgctccgttt ggcctgctcg taatccatca aattgggccc tcgggcgcga
      601 gcctcgttgt cttgaaagca cagctcgacc tgcttgcact gctcgcgggc cgccgacccg
      661 ccatagccgg ccttaagctt taccccgttg tagcgcgggg cattgtgaga ggcggtgatc
      721 atgatcccgg caattgcccc gagctggcga acggcgaacg aggcggtggg agtaggtaca
      781 tcggcgctcg tgagtgaaac cgtgaaaccg ttgccggcca acactcgggc cgcttccacc
      841 gcgaagcggt ccgataagaa acgggtgtcg aagccgacga ccatccgccg cggatcgcca
      901 gcgggttggc cgcgcgggac ccaatcttca gaggccaccc catccgcgat cgcctggctc
      961 accatccgga ggttgtggaa ggtgaacgtg tcgccgataa ccccgcgcca gccgtcagtt
     1021 ccgaagtgaa tggtcatgcc caagctccaa ccgggtcatt atactgaggt caaaaggacc
     1081 cctatgctag aatggcttgg ccatgcccca accgctgctt gaaggcattc ccgccaaacc
     1141 cggctgctac ctgatgaaag acgagagcgg aaaggtgatc tatgtcggca aggcggtaaa
     1201 cctgcgcagc cgggtccgct cctacttcca cgagtccggt tcggccgacc gcaagacggc
     1261 tgaactggtg gcgcgggtcg ccgacgtgga atggatcgtg gtggcctcgg agctcgaggc
     1321 cttgatcctc gagatgaacc tgattaagcg ccaccggccg aagtacaacg ttcgcttgaa
     1381 agacgacaaa cgctacccct acgtgcgagt gcattggggg gatccatttc ccaaggtcac
     1441 catgacccgg caggtcgtgg gggacggaag ccgatactac gggccctaca caagcgtgtg
     1501 ggccgtgcat cagaccctgg acgtgctgcg ccggatattt ccctatctga cctgtgatcg
     1561 agtgatcacc gggcaagact tgcgggcctg cctctaccac gacatcaagc tgtgccttgc
     1621 gccgtgcatt ggtgcggtgg atcaggccgg ctaccgggaa atgatcgggg atctttgcgg
     1681 cttcctggaa ggccggaccg aaccggtgat ccagcggctg caagccgaga tggcccgggc
     1741 ctcgggcgcg atggaatatg agcgggcagc tgcgattcgg gaccagctga cagcgatcga
     1801 ccgggtcatc gaaggtcaaa aggtagtctc ccgggagaac ctggactcgg atgtcattgc
     1861 gttcgcccgg gatgaccgca gtgcctgtgt gcaggtgttc ttcgtgcggg gcggaaaact
     1921 aatcgggcgg gagtacttcg tgctcgaggg cacccggcag gccggcgacg aagaggtggt
     1981 ccgggccttc gtcaagcagt tctacgccaa ctcgagcacg gtcccccaac gggtgctgct
     2041 gccgctggaa atcgaagagg cggcgatcat cgaagcctgg ctgaaccgcc gcagcccgga
     2101 acacaaggta aagatcacgg tcccgcggaa gggtgtgaaa cgggagctgg tggaaatggc
     2161 ggccgaaaac gcagccgaga ccctctcgat gctaaaagcc cgctgggagg ccgatcgcag
     2221 caagcacgta caggcgctgg ccgagctgca agccgcgctg cagctgggcg agccgccggt
     2281 tcggattgag ggctacgaca tctccaattt gcagggaacc gcggccgccg gttcgatggt
     2341 ggtgttcgaa aacggcgcgc cgaacaaagc gctctaccgc aagtacacga tcaaatccgt
     2401 gag
//
LOCUS       DATDPQ010000166         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_854481, whole genome shotgun sequence.
ACCESSION   DATDPQ010000166 DATDPQ010000000
VERSION     DATDPQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854481"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VI520_03905"
     CDS             complement(<1..369)
                     /locus_tag="VI520_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLF37061.1"
                     /translation="MSEVGLWLQVMPSLTAILDSLRSGNRAYLEELQVRFETVEPELQ
                     AFLPEEGRFERLHTEFGELEKSCPAVEDRPPLFGLPIGVKDIFRVDGFETRAGSRLPA
                     ASLAGEQAVCVSQLKRAGGVV"
     gene            complement(366..731)
                     /locus_tag="VI520_03910"
     CDS             complement(366..731)
                     /locus_tag="VI520_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF37062.1"
                     /translation="MPRVVHFELGVADPERAAQFYRTVFGWKVERWGDFDYWLVTTGE
                     EGEPGINGAILVNKDAKPRTVNTVQVESIEDAAEKVLDAGGEVVTEKVTVPNFGYNLF
                     CKDTEGNLFGLHQFDPQAK"
     gene            complement(807..1289)
                     /locus_tag="VI520_03915"
     CDS             complement(807..1289)
                     /locus_tag="VI520_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLF37063.1"
                     /translation="MDNLQLNLTVNGQPTIRQVESRLLLSDFLRHELGLTGTHVGCEH
                     GVCGACTVQFDGRPVRSCLMLAVQAEGHAVTTVEALANSQEQLHPLQQAFWEAHGLQC
                     GFCTPGFLMTLVPFLAEHPNPSEREIREAISGNLCRCTGYQNIVAAVRLAAERGSTGW
                     "
     gene            complement(1319..>2230)
                     /locus_tag="VI520_03920"
     CDS             complement(1319..>2230)
                     /locus_tag="VI520_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HLF37064.1"
                     /translation="RETMKPPPFKYLAPDSLEQALELMAQYGEEAKLLAGGQSLIPAM
                     NFRMAQPAVLVDLNRITSLGYIKTNGDGSLAVGAMTRQRELEFDPQVAERVPLLHECM
                     PHVAHPQIRNRGTLGGSLAHADPAAELPVVALAASARIRAESSQGERWIEAQQFFQGM
                     FTTALQPGEILVEVVFPPAEPGTGWSFLEASRRRGDYAMMGVAAGLSLNAEGVCRAAR
                     LVYMNAGDGPIEARQAAAGLGGQRPGPEAYEQAAAAAQQEIDPLGNVHASVDFQRHLA
                     TVLTRRALALAAERARGNGSSRVADAT"
BASE COUNT          385 a          794 c          654 g          397 t
ORIGIN      
        1 caccaccccg ccggcccgtt tcagttgaga aacacagacc gcttgctcgc cggccaggct
       61 cgcggcgggc agccggctcc cggcccgggt ctcaaagccg tcgactcgaa aaatgtcttt
      121 caccccgatc gggagtccaa aaagcggggg ccggtcttcc acggctggac aagacttctc
      181 gagctcgccg aattctgtat gcagccgttc gaagcgcccc tcctctggca ggaaggcttg
      241 cagttcaggc tcgaccgtct cgaaacgcac ctgcagttcc tccagatacg ctcgattgcc
      301 cgatcggagc gaatcgagga tcgcggtcaa cgacggcatc acctgcagcc agagccccac
      361 ctcgctcact ttgcctgggg atcgaactgg tgcaggccga acaaattccc ctccgtatcc
      421 ttacagaaca ggttgtaccc gaagttgggg acggtgactt tctcggtcac gacctcgccg
      481 cccgcgtcga gcaccttctc ggcggcatct tcgatcgact cgacctgcac cgtgttcacc
      541 gtgcggggtt tggcatcctt gttgaccagg atggcgccgt tgatccccgg ttcgccctcc
      601 tccccggtgg tgaccagcca atagtcgaaa tccccccacc tctccacctt ccatccaaat
      661 acggtccgat agaactgggc ggcccgctcg ggatcggcca cccccagttc aaaatgaact
      721 actctcggca tcggctgccc tccttccgca ttgcttccgg caaccaggcc ggtgtctccg
      781 gttgcaatcc gcccggacgc ttccgctcac cagccagtgc tccctcgctc ggccgccaaa
      841 cggaccgcgg ccacgatgtt ctgataaccc gtgcagcggc agagattgcc cgaaatagcc
      901 tcccggattt cccgctcgct cggattgggg tgttcagcca ggaaaggcac cagagtcatg
      961 aggaaacccg gcgtgcagaa tccacactgc aagccgtgcg cctcccagaa tgcctgctgt
     1021 agcggatgga gctgctcctg actgttggcc aacgcttcga ccgtagtgac cgcatgcccc
     1081 tcggcttgaa ccgccagcat gaggcaggag cggaccggtc ggccgtcaaa ctgcaccgtg
     1141 caggccccgc acaccccatg ttcgcagccg acatgggtac cagtcagccc cagttcatgc
     1201 cgcaggaagt cgctcagcag cagccgcgac tccacctgac ggatcgtcgg ctggccgttg
     1261 accgtgaggt tgagttgaag gttgtccatc ctacgctcgg tgggtctcgg aggatcgctc
     1321 aggtggcgtc cgccacccga gagctcccat taccccgggc gcgttcggcc gcgagggcca
     1381 gcgccctgcg ggtcaatacc gtggccaggt ggcgttggaa atcgacactg gcgtgcacat
     1441 ttcccagcgg atcgatctcc tgctgcgcgg cggccgccgc ctgctcatag gcctccggcc
     1501 cgggccgctg tccaccgagc cccgccgcgg cctggcgagc ctcgatcggc ccatcccccg
     1561 cgttcatata cacgagccgg gccgcgcggc agaccccctc ggcattcaag ctgaggccgg
     1621 ccgcaacccc catcatggcg taatcccctc gccgccggga ggcttccaga aagctccagc
     1681 cggtcccggg ctcggcgggt ggaaagacga cctccaccag gatctccccg ggctgtaggg
     1741 cggtggtgaa cattccttga aagaactgtt gcgcctcgat ccagcgctca ccctgggaac
     1801 tctcggcccg gatccgggcg gaagcggcca gcgccaccac cggcaattcc gccgccggat
     1861 cggcgtgggc caggctacct cccagagtgc cgcggtttcg gatttgtgga tgggcgacgt
     1921 gcggcatgca ctcgtgcagc agcgggaccc gctccgccac ttgcgggtcg aactcaagct
     1981 ccctctgccg agtcatggcg cccactgcca gcgaaccatc tccattcgtc ttaatgtagc
     2041 ccagggacgt gatccgattg aggtccacca gcacggccgg ctgcgccatg cggaagttca
     2101 tggcggggat gaggctctgc ccaccggcca gcagcttcgc ctcttccccg tattgggcca
     2161 taagctccag cgcctgctcc aacgagtcag gcgccagata cttgaagggc ggcggtttca
     2221 tggtctctcg
//
LOCUS       DATDPQ010000167         5348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2418302, whole genome shotgun sequence.
ACCESSION   DATDPQ010000167 DATDPQ010000000
VERSION     DATDPQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418302"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VI520_03925"
     CDS             complement(<1..830)
                     /locus_tag="VI520_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37065.1"
                     /translation="MAKKTARKATKKPVKRTNSYAAKDIQVLEGLEAVRRRPGMYVGG
                     TDIRGLHHLIFEVVDNSIDEALAGACDRINVRIHPDSSVTVEDNGRGIPVEKHPRIKK
                     PALEVVMTMLHSGAKFGGDGYKVSGGLHGVGVSAVNALSEWCEVQVRRDGKVHAQRYA
                     RGVPAGPVEVVGKAAKADTGTTTTFLYDREVFKGNLEYRFETLVQRFREMAFVTRGVM
                     IYFVDERVEREMTFYFEGGIVSFVKYLNRNRHVLHPVVHADRQVAGTGIEVALQYTDA
                     "
     gene            complement(848..1810)
                     /locus_tag="VI520_03930"
     CDS             complement(848..1810)
                     /locus_tag="VI520_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37066.1"
                     /translation="MDSQAGSKPIGVLAAFATGFDRVAARPLLIIPPLLIDLLLWFGP
                     HFRTAGIFGWVQDQALRASGLAGEQAALVRESIRSLSERFNLLSSLSTLPFGVPSLMA
                     GRMPVLNPLGQANPLEVAQPGLAILLWLSFSALGLAFGAYYQHWIAQAIASEAELGST
                     WKAAIRMVGFGFVLFIGAIATATSVGLAFGVASLILPLFGVGVLYLGLILMFWLAVYL
                     AFTPHGIVRYNLGVVRAMLESIVLVRWNLLSTVGFLLLGFGAYWLTGLIWALPAESSW
                     FSVLAVIGHGFVSTTLVAGSYAYYQGRREWLEQARALRSRPPIA"
     gene            1847..2689
                     /locus_tag="VI520_03935"
     CDS             1847..2689
                     /locus_tag="VI520_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HLF37067.1"
                     /translation="MAGPWIALLTDFGTQDTSAGVLRGVLATLAPHASLLDLTHDIPA
                     GDVRAAAFALWQATPYFPPQTIFICVVDPGVGSTRRALAVAWKERICVGPDNGLFSYL
                     IERDGPAEAVELAGSGYQLPEPSPTFQGRDVFAPAAAHLASGVKLADLGSPASDLVHF
                     ATPRLQAVETSGIEGEVLHADRFGNLMTSIGVLRREAEGLTLEPWLGQGPTRRVVGSD
                     PRVRLASGEELDLLRTYSDVPPGRGLAYIGSSGLVEIGINQASAAAGLKIAPGDTVQL
                     RVRN"
     gene            2692..4002
                     /gene="murA"
                     /locus_tag="VI520_03940"
     CDS             2692..4002
                     /gene="murA"
                     /locus_tag="VI520_03940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLF37068.1"
                     /translation="MASFRIEGGHPLNGEVTPSGNKNAALPLMTSCLMTEEPVTLHNV
                     PDIGDVDIMRSLLGSLGVELEQLGPGSLRLRAHAAVRARLDPELCRRIRASILLAGPM
                     LSRFGGIDLPPPGGDVIGRRRVDTHLLALKNLGAEVGYNEREFHMEVKGRLRGGEVLL
                     DEASVTATENAIMGAVRAKGSSVIRNAASEPHVQELCHFLNGLGAQIRNIGSNTLEIE
                     GVDRLHGGEFTIGPDYLEVVSFIAAASVTGGSVWIRKAGPQHLDMIRLVFNRLGVDWV
                     EKEQDLLVAARPLLEIQSDLGGFVPEITVMPWPAFPTDLVSIAIVVASQAKGSVLFHD
                     WMYPSRMFFVDKLVSMGARIILCDPHRCIVEGPTQLIGEHLESPDIRAGMALVLAALA
                     AKGTSTIRNVGQIERGYERIDEKLRQLGAQIERINDSVPAGSVD"
     gene            4069..4245
                     /gene="rpmH"
                     /locus_tag="VI520_03945"
     CDS             4069..4245
                     /gene="rpmH"
                     /locus_tag="VI520_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HLF37069.1"
                     /translation="MPKRTYQPKIRRRVRVHGFRARMASKAGREVLRRRRRKGRYRLA
                     VRKNAHVKKTNWNG"
     gene            4277..4645
                     /gene="rnpA"
                     /locus_tag="VI520_03950"
     CDS             4277..4645
                     /gene="rnpA"
                     /locus_tag="VI520_03950"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HLF37070.1"
                     /translation="MDRKFRLTSATDFKRVRRTGKSYAHPLVFLIASPNGGKPSRIGV
                     SASQSFGNAVKRNRAKRRMREAVRVLYAAVQPGWDLILTARPGLDQAGWPELLEAIGG
                     LFRRAHLANGGSKSRNGRTA"
     gene            4629..4961
                     /gene="yidD"
                     /locus_tag="VI520_03955"
     CDS             4629..4961
                     /gene="yidD"
                     /locus_tag="VI520_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLF37071.1"
                     /translation="MDALHESRIAAVVGTAGGEHGHPHPDEPALREIPVTLATLPRLP
                     ALALIRFYQATLSRTLPSGTCRFTPTCSHYSYQAIAKFGLLKGSALSAWRILRCQPFS
                     KGGYDPVP"
     gene            4975..>5348
                     /locus_tag="VI520_03960"
     CDS             4975..>5348
                     /locus_tag="VI520_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLF37072.1"
                     /translation="MIAKLPVSLLPTVVLFAAALLLAGCAGAGVQGASSWPGLASDGD
                     LAYVAYNQAVYAVDLESGALRWTYPVAAERGRAFYAPPAVSADGLLFVGDFLNQITAL
                     DSADGSVEWGPLQLSTRNQRIIG"
BASE COUNT         1011 a         1716 c         1666 g          955 t
ORIGIN      
        1 taggcgtcgg tgtactgcag agctacctcg atcccggttc cggccacctg ccggtcagcg
       61 tggaccaccg ggtgcaacac atgccgattg cggttgaggt acttgacgaa cgagacaatc
      121 ccgccttcga agtagaaggt catctcacgc tcgactcgct catccacaaa atagatcatg
      181 accccccggg tcacgaatgc catctcacga aagcgctgca ctagagtctc gaatcggtat
      241 tccagattgc ccttgaagac ctcccgatcg taaaggaaag tggtcgtggt gccggtgtct
      301 gctttcgcgg ccttgccgac cacttcgacc gggccggcag gcaccccgcg ggcgtagcgc
      361 tgggcgtgaa ctttgccgtc ccgtcggacc tgcacttcgc accactcgga gagagcgttg
      421 acggccgaaa ctccgacccc gtgcagcccg ccggagacct tataaccgtc gcccccaaat
      481 ttggcgccgg agtgcagcat ggtcatcacg acttccaagg ccggcttctt gatccgcggg
      541 tgcttttcga cagggatccc gcgtccgttg tcctccaccg tcacgctgct gtcgggatga
      601 atcctcacgt taattcggtc acacgccccg gccagcgcct cgtcgatcga gttgtccacc
      661 acctcaaaga tcaggtgatg caggccccga atatcggtgc cgcccacata catccccggg
      721 cggcgtcgaa cggcctccag tccttccaag acctggatgt ccttggctgc gtaactgttg
      781 gtcctcttga ccggcttctt ggtggccttg cgggccgtct tcttcgccaa agtgatactc
      841 cccagtccta ggcgatcggc gggcggctgc gtagggccct ggcttgctcc agccattccc
      901 gccggccttg atagtaggcg taactgccgg ccaccagggt tgtgctgaca aacccgtgcc
      961 cgatcacagc cagtactgaa aaccaggagc tctccgccgg aagcgcccag attaggcccg
     1021 tcagccagta ggctccgaag ccgagcagca ggaatccaac tgtgctcagc aggttccagc
     1081 gcaccagcac gatgctctcc agcatggccc ggaccacgcc caggttgtag cggacaattc
     1141 catgcggcgt gaaagccagg tagaccgcga gccaaaacat caggattaaa cccagataga
     1201 gcacccccac tccgaacagc gggagaatca atgacgcaac tccgaaagca agtcccaccg
     1261 aggtggcggt cgcgatcgcc ccgatgaaga gtacgaaccc gaatcccacc attcggatgg
     1321 cggccttcca agtcgacccg agctctgcct cggaggcgat ggcttgcgcg atccagtgct
     1381 ggtaataagc gccgaaagcc aggcccagcg cggagaacga cagccagagc aagatggcca
     1441 accccggctg ggcgacctcg agcgggttcg cttggccgag tgggttgagt acaggcatcc
     1501 gaccggccat caagctgggg acgccgaatg gaagtgtgct cagcgaggag agcaagttaa
     1561 agcgctcact caacgagcgg atgctttccc gcactagggc cgcctgctcc ccggccaacc
     1621 cgctggcccg caaggcttgg tcctgaaccc agccgaaaat gccggccgtc cgaaagtggg
     1681 gtccgaacca gagcaggagg tcaattagca gcggagggat gatcagcagc gggcgggcgg
     1741 cgactcgatc gaagcccgta gcgaaggcag ccaacacccc gatgggcttc gagccggctt
     1801 gcgaatccac gaaaaccgat tctaccatta caatgcccca actgctgtgg ccggaccttg
     1861 gatcgcgctg cttacggatt ttggtaccca agatacctcc gccggcgtcc tgcgaggggt
     1921 cctcgccacg cttgccccgc acgcctcgct gctggacctg acccacgaca tcccagccgg
     1981 agacgtgcgg gccgccgctt tcgctctgtg gcaagccacc ccgtatttcc cgcctcagac
     2041 catctttatc tgcgtcgtcg atccgggagt cggctcaacc cgccgagccc tggccgtcgc
     2101 ctggaaagag cggatttgcg tcggccccga taacggcctg ttcagttatc tgatcgagcg
     2161 ggatggccca gcggaggcgg tcgagcttgc cggatctgga taccaacttc cggaaccgag
     2221 cccgaccttc caaggtcgag atgtttttgc gccggccgcc gcccacctgg cctccggtgt
     2281 caagctcgcg gatcttggat cgccggcgag cgatcttgtt catttcgcca ctccccgcct
     2341 gcaagccgtc gagacctccg gcatcgaagg agaagtgctg cacgcggatc ggtttggcaa
     2401 cttgatgacc agcattggag tcctgcgccg cgaagcagaa ggcctgacct tagaaccctg
     2461 gctgggacag gggccgaccc ggcgggttgt gggaagcgat cctcgggtgc gcctggcctc
     2521 gggtgaggag ttggacttgc tgcggaccta cagcgacgtc ccgccgggcc gcgggctggc
     2581 ctatatcggc agcagcggcc tggttgaaat cgggatcaat caagcctcgg ccgccgccgg
     2641 actgaagatc gctcccggcg ataccgttca gcttcgagtc aggaactagg catggcttcg
     2701 ttccggatcg aaggtgggca ccctctcaac ggcgaagtca ctccctccgg aaacaagaac
     2761 gccgccctcc cgctgatgac ctcctgcctg atgaccgagg aaccggtgac cttgcacaac
     2821 gttccggaca tcggggatgt ggacatcatg cggagtttgc tcggcagcct gggagtcgag
     2881 ctggagcagc tcggcccggg ctcactccgc ctgcgggccc atgctgcggt gcgagcccgg
     2941 ctcgatccgg aattgtgccg ccggatccgg gcttcgatcc tgttggccgg accgatgctg
     3001 agccgcttcg gcgggatcga tctgccgccc ccgggcgggg atgtgatcgg ccgccgacgg
     3061 gtcgacactc acctgctagc gctcaaaaac ctgggcgccg aggtcgggta caacgagcgc
     3121 gaattccata tggaggtcaa ggggcggctg cgcggtgggg aagtcttgtt ggacgaggcc
     3181 agcgtcacgg cgaccgagaa cgcgatcatg ggggcggtgc gggccaaggg cagctctgta
     3241 attcgcaacg cagcttcgga accgcacgtc caggagctct gccacttctt gaacggcctc
     3301 ggtgcccaaa tccgaaatat cggatctaac actctggaga tcgaaggagt cgaccggctg
     3361 catggtggcg agttcacgat cgggccggac tatttggagg tggtcagctt catcgcggcc
     3421 gccagcgtga ccggcggttc ggtttggatt cggaaagccg ggccccaaca cctggacatg
     3481 atccggctgg tattcaatcg gctcggcgtg gactgggtcg agaaagagca ggacctcctg
     3541 gtagcggccc gacccctgct ggagatccaa tccgacctgg gcggattcgt tcccgagatc
     3601 acggtgatgc cgtggccggc cttcccaacc gatctggtca gcatcgccat cgtggtagcc
     3661 agccaggcca agggatcggt ccttttccac gactggatgt accccagccg gatgttcttc
     3721 gtcgacaagc tggtctccat gggcgcccgg atcattctgt gcgacccaca ccgctgcatc
     3781 gtcgaggggc cgacccaatt gatcggtgaa cacctggaga gcccggacat tcgggcgggg
     3841 atggcgctgg tattggccgc cctggcggcc aaggggacct ccactatccg aaacgtcgga
     3901 cagatcgagc ggggatacga acgaatcgac gaaaagcttc gtcagctcgg tgcacagatc
     3961 gaacggatca acgattcggt gccggctggg tcagtcgatt gaccccttca ctcagcatgc
     4021 ggtataatcc atcggcgctt cagccaagcg cgagagccga accaacgaat gcctaagcga
     4081 acctatcaac ccaagatccg ccggcgagtc cgagtccacg ggtttcgagc ccgaatggcg
     4141 agcaaggccg gcagggaagt tctccggcga cgcaggcgga aaggccgcta ccgccttgcc
     4201 gtccgcaaga acgcgcacgt taagaagacc aactggaacg gctgacgacc ggatcggggt
     4261 tatcagcgag ccccgggtgg accggaagtt caggctaacc agcgcaacgg atttcaagcg
     4321 ggtgcggcgt actgggaagt cttatgccca cccgctcgtt tttttgatcg ccagccccaa
     4381 tggaggaaag ccatcccgga tcggggtgag cgctagccag tcgttcggga acgcggtcaa
     4441 acgcaatcgg gccaagcggc ggatgcggga agcggtgcgg gtgctctacg ctgccgttca
     4501 accaggctgg gacctgattc tcaccgcccg gcccgggctc gatcaggctg gctggcccga
     4561 actgctcgag gccatcggcg gattatttcg ccgggcccat ttggccaatg gagggagcaa
     4621 gtcgcgcaat ggacgcactg catgaaagcc ggatcgctgc cgtggtgggt acggctggag
     4681 gggaacacgg gcacccgcat ccggacgagc ctgccctgcg ggagatcccg gtcactctcg
     4741 ccactctgcc acggctaccg gccctggcgc tgatccggtt ctatcaggcc accctatccc
     4801 gaaccttgcc gtccgggaca tgccgcttca caccgacttg ttcacactac agctatcaag
     4861 ccattgccaa atttggactg ctcaagggct cggctctctc ggcctggcga atcttgcgct
     4921 gccaaccctt cagtaagggt ggctacgatc cggtcccgtg aggtctctgc ccgcatgatc
     4981 gcaaagctgc cggtctccct actgccgact gtagtgctgt ttgccgcagc gctgctgctc
     5041 gccggatgcg cgggagccgg ggttcagggc gcatccagtt ggccgggttt ggccagcgat
     5101 ggcgatttgg cctacgtggc ctacaatcag gcggtctacg cggtcgatct cgagtccggc
     5161 gccttgcgct ggacctaccc ggtcgcggcg gagcggggcc gggccttcta cgcgccgccg
     5221 gctgtctcgg cagatggctt gctattcgtg ggcgatttcc tgaatcagat cacggcgctc
     5281 gactcggcgg acggctcagt ggaatgggga ccactccaac tttcaacccg caatcagcgg
     5341 atcatcgg
//
LOCUS       DATDPQ010000168         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_589386, whole genome shotgun sequence.
ACCESSION   DATDPQ010000168 DATDPQ010000000
VERSION     DATDPQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589386"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2856)
                     /locus_tag="VI520_03965"
     CDS             complement(<1..2856)
                     /locus_tag="VI520_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLF37073.1"
                     /translation="MLVSLIDRLTGASLRFRWIVIALAIVVLPAGVVALTQLDLELIP
                     PLEFPQTVILGVNQGMQAQEMLDQVTRPIEAAVQGVEGVVNVESTSGAGLSVVIVRNE
                     FGIDQAALRDELRAVLGLVSLPDEMQAPELIEFGLSDLPIAFVSVSSSSFSLVELKEF
                     VAAEIVPELEALEGVADVQVSGGQELPEPVVIPTAEPTPTSEPTQVPQGMPLPQAWID
                     LAASQGVTLRTTGDLTPEMVGGVAAVAPELLNDFTPELILATAPEVLAAIPAAVVAQL
                     TPEQQQALVQQLEGFVSEEPAEEAGELPTVWQQAGQAQGIELVLPEDVTPQIMQGIAQ
                     FAPQLLDLLNADHLRRFAPEVLAWLPASYIETLEADVRAELEGLAEPAGGLGALAAAP
                     PVGDQFTDAPPLAGEWLQPPENDPNAPPFFQTAANLLQNGFAPTAAEFLNLLVSNSGP
                     STPELMRDLSPEAILWLADNEDGFLETLTASVLRLLSQEALAALPGEFLESLDPALAA
                     ELRAIAAGELEVFIPTDTINRVNGNPSLGLQLFKTSEANTVGASREAFNKLEQLEAAQ
                     AGLQFNIVFEQASFIEESITGVAREGGLGAIFAVIVILIFLSGTVNGKYRLSWRSTLV
                     TAVSIPLSIFMAFAAFRWLPPVADLVLGPLDAATGNIPVLGSAVTALHRLFPLGITLN
                     IMTLSGLTVAVGRVVDDSIVVLENIYRHIQRGDDRKQAVLIGTRDVAIAILASTVTTV
                     VVFLPIGLLGGVVGQFFLPFGVAVTYALGSSFLAAVTVVPLLAYLFIRKEHLPPEAET
                     RLQRSYTPLLQWALVHRGQVLAIAGVLFAGSLFLLSQRPQAFLPELGEVQISANISLP
                     DGTAMAETDRRVAEFESELAGITGVDTVSSEIGTGGGLQNLLLGASIDQGAATVSAGV
                     EQGADLDQLTAEVRELAVGVFGEENAIVSGGTLSGSAF"
     gene            complement(2919..3107)
                     /locus_tag="VI520_03970"
     CDS             complement(2919..3107)
                     /locus_tag="VI520_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37074.1"
                     /translation="MVPKRITKLIPALLLILVGIGWFLQGINVLPGSFMTGQPEWAVA
                     GGLAFLAGLWIIRRSRRP"
BASE COUNT          641 a         1011 c         1013 g          549 t
ORIGIN      
        1 aaacgcggaa ccagagaggg tgcctccgga cacgatcgcg ttttcctccc cgaagacccc
       61 cacggctagt tcgcgtacct ccgcggtcag ctgatcgagg tctgcaccct gctccacgcc
      121 ggcgctcacc gtggcggcgc cttgatcgat cgaggcgccc agcagcaagt tctgcaggcc
      181 ccctccggtc ccgatctcgg aggagaccgt gtcgacgccc gtgatgccgg ccagctccga
      241 ttcgaattca gccactcgcc gatccgtttc cgccatggcc gtgccgtcgg gcagagagat
      301 gttcgccgag atctgcacct cgcccagctc aggcaggaat gcctgcggcc gctggctcaa
      361 caaaaagagg ctgccggcaa agagcacgcc ggcaatcgcc aagacctgac cacgatggac
      421 cagtgcccat tggagcaacg gggtgtatga gcgctgcaag cgggtctcgg cttccggtgg
      481 gaggtgctcc ttgcggatga acaggtaagc cagtagtgga acgactgtga ctgccgccag
      541 gaaactggag cccaatgcgt aggtaaccgc caccccaaac ggaaggaaga actgtccgac
      601 caccccgcca agcaggccga tggggaggaa cacaacgacg gtggtaacgg tggaggccag
      661 gatggcgatc gcgacatcgc gggttccgat caggacggcc tgcttacggt cgtcgccccg
      721 ctggatgtgc cgatagatgt tttccaacac cacgatcgag tcgtcgacca cccgcccgac
      781 cgccaccgtc agccccgaga gggtcatgat gttcagcgtg atgccaaggg ggaagagcct
      841 gtgcagagcc gtcaccgcgc tgcccagtac agggatgttt ccggtggcgg cgtccagcgg
      901 ccctaacacc aggtcggcca ccggcggaag ccagcggaag gcggcgaagg ccatgaagat
      961 ggagagcggg atgcttacgg cagtgaccag cgtactcctc caggacagcc ggtacttgcc
     1021 gttgacggtc ccgctgagga agatcaggat cacgatgacc gcgaagatcg ctcccaaacc
     1081 accctcacgc gcgacgccgg tgatcgattc ttcgataaag gaagcctgct caaacacgat
     1141 gttgaactgc agcccggcct gggcggcctc cagctgctcc agcttgttga aggcttcgcg
     1201 ggaggcgccg accgtgttgg cttcactcgt cttgaagagc tgcagaccca gactgggatt
     1261 gccgttaacc cggttgatcg tgtcggtcgg aatgaagact tccagctcac cggccgcgat
     1321 ggcccggagt tccgccgcca gcgcgggatc gagactctcc aggaactctc cgggcagagc
     1381 tgccagggct tcctgggata gcaatcggag caccgatgcg gtcaaggtct ccaagaatcc
     1441 atcttcgttg tctgcgagcc agaggatggc ctctggagag aggtcccgca tcagctcggg
     1501 ggtggacggg ccgctgttgg agaccagcaa gttgaggaac tctgcggcgg tgggcgcgaa
     1561 tccgttctgc agcagattgg ccgcggtctg aaagaacggt ggcgcattcg ggtcgttctc
     1621 aggcggctga agccactccc cggcaagcgg cggggcgtcc gtgaactgat cgccaacggg
     1681 gggagctgca gcaagcgctc ctaatccccc ggctggttca gccaaacctt cgagttcggc
     1741 ccgcacgtcg gcctccaggg tctcgatgta acttgccggc agccaagcca gcacttcggg
     1801 cgcgaagcgc cgcagatgat ccgcattcaa caggtccagc aattgtggag cgaactgggc
     1861 aatcccctgc atgatctgcg gagtgacgtc ctccggaagg accagctcga ttccttgtgc
     1921 ctggccggct tgctgccata cggtcggcag ttcgccggcc tcctcggccg gttcctccga
     1981 aacaaatcct tcaagctgct ggacgagggc ctgctgctgt tccggtgtga gctgagcgac
     2041 cactgccgcg ggaatagccg ccaggacttc cggcgccgtg gccaggatca gctcaggggt
     2101 aaagtcgttc aacagctcag gggccaccgc ggcgacgccc ccgaccatct caggggtcag
     2161 gtcgccggta gttctcagcg tgactccctg gctggcggcg aggtcgatcc acgcctgggg
     2221 gagcggcatg ccttgtggca cctgggtcgg ctcgctagtg ggggtcggtt ctgcagtcgg
     2281 aatgactacc ggctccggca gttcctggcc gccggacacc tgcacgtcgg ctacgccctc
     2341 cagcgcctcc agttcaggga cgatttcagc ggcgacgaat tccttgagct cgaccagaga
     2401 gaaactcgac gaagaaacgc tgacgaatgc aatcggaaga tccgaaaggc caaactcgat
     2461 caactccgga gcctgcattt catccgggag ggaaacgagg ccaagcacgg ctcgaagttc
     2521 atcccggaga gcagcctgat cgatgccaaa ctcgtttcgc acgatgacca ccgagagtcc
     2581 tgcgccgctg gttgactcca cattgacaac accttcgaca ccttgcacgg cagcctcgat
     2641 cggccgggtg acctgatcaa gcatctcctg ggcctgcatt ccctgattaa cccccaggat
     2701 cacggtctgc ggaaactcga gcggcggaat cagttcgaga tccaattggg ttagtgcgac
     2761 cacgcctgcc ggcagcacca cgatggccag cgcgatcaca atccacctga agcgcagcga
     2821 ggccccggtc aggcggtcaa tgagcgatac gagcatgttc tttctcctcc tgtcagagtt
     2881 cggaagctat tgatcaacgg gtgagtgact taacggtctc aggggcggcg ggaccggcgg
     2941 attatccata gcccggctag aaacgccagc ccgccagcga ccgcccactc gggctgaccg
     3001 gtcatgaagc tgcccggaag cacgttgatc ccttggagga accaaccgat ccccaccagg
     3061 atgagcagga gcgcagggat taacttcgtg attcgcttcg ggaccaactc cttgctcttt
     3121 gtgatggacg gtgtcagcta gccgagcagg cggccttctc gctctggacg aatccagcgg
     3181 cgggaccaaa gggtgagcag gtcaatcatg ggcc
//
LOCUS       DATDPQ010000169         3120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1647299, whole genome shotgun sequence.
ACCESSION   DATDPQ010000169 DATDPQ010000000
VERSION     DATDPQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647299"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VI520_03975"
     CDS             <1..987
                     /locus_tag="VI520_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLF37075.1"
                     /translation="NNPLLNRATLGAYPTGSVFKMITMAAALESGFYTPDTVYNCGLE
                     FTELTGVTLFDWRFDVELPAAGEITLSQGLERSCNPYFFHIGLDLFNRGLETAISDMA
                     RGFGLGEPTGIEIGEASGLLPDPENKLALYGDPWNRTDSVMLSIGQSYLQATPLQVAR
                     FVAAIGNGGTLYRPQLVSSIQNAEGEVLSSFSPVVQGTLPVSAENLAAIQQAMIRVIR
                     DPRATAYRPFLGIPDINIAGKTGTAETGEFTDTHAWFGGYTFEERSDRPDIAVVVVLE
                     YQGQGSEWAAPVFKRVVEAYFKGGPISRYPWEARIRVPATPEPDPEEQGTEG"
     gene            1020..1913
                     /gene="rsgA"
                     /locus_tag="VI520_03980"
     CDS             1020..1913
                     /gene="rsgA"
                     /locus_tag="VI520_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HLF37076.1"
                     /translation="MILRARAGFYDVETEQGVVTAKLRGRLKKLAPRAELAAIGDRVK
                     LSLFEDGSASIEEILPRQRALSRRAPGREIEQILVANPDQAVFVFACADPDPNFRMLD
                     RMLVGAERETIPAVICANKIDLVQPRSAREEFDVYENIGYRVLYTSATTGKGVGDLRR
                     ELSGKLSVLAGPSGVGKSSLLNMVQPGLGLRTDAVSQATGKGIHTTVARELLPLTGGG
                     YVADTPGLKAFALWDIEPDELDAYFREIAPLVEGCEFSDCTHEHEPGCAVRAAVEAGT
                     IDVNRYESYLRMRSGEPFIER"
     gene            complement(1942..2361)
                     /locus_tag="VI520_03985"
     CDS             complement(1942..2361)
                     /locus_tag="VI520_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF37077.1"
                     /translation="MKSGPEPLRQIVINRGVLSLALAGTGLLLLSLVPASENPGGGPT
                     PPPAVSAAATDDPAAYGRALFQAKGCVGCHQHNAVNPGSGVSVGPELSSRSYDPDFLR
                     SWLKDPPAVRPGTQMPNLRLSAEEIEALIAFLVSESS"
     gene            complement(2345..2881)
                     /locus_tag="VI520_03990"
     CDS             complement(2345..2881)
                     /locus_tag="VI520_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37078.1"
                     /translation="MSRRVLGSLIVLALAFALVPAAAAGGWAIATLDQLPGEVQAGES
                     VSLGFMVRQHGKTPTNEVDPYVLAYNPESGESVRAYAVQEGEVGHFVVEIVFPAAGTW
                     EWSIVPAPFEGTQLEPLSVLPATLTQPAASPEQTVTLGASDLRGWLRWSGIALLLGAG
                     ALAVLGRRGARQTVEERA"
BASE COUNT          590 a          983 c         1014 g          533 t
ORIGIN      
        1 aacaatccgc tgctcaaccg ggcaaccctc ggggcgtatc cgaccggctc ggtgttcaag
       61 atgatcacga tggccgccgc gctcgaatcc gggttctaca ctcccgacac cgtgtacaac
      121 tgcggcctcg aattcacgga gctgaccgga gtcacgctgt ttgactggcg cttcgacgtg
      181 gaattgccgg ccgccggcga gatcaccttg agccagggtc tggagcggtc ctgcaatccg
      241 tacttcttcc acatcggctt ggatctcttc aatcggggtc tggagaccgc catctccgat
      301 atggcccggg gctttggcct gggggagccg accggtatcg agatcgggga ggccagcgga
      361 ctgctgccgg atccggagaa caagctcgca ctctatggtg acccgtggaa ccggaccgac
      421 agcgtgatgc tctcgatcgg gcagagctat ctgcaggcga ccccgctgca ggtggcccgc
      481 ttcgtggccg cgatcgggaa tgggggcacg ctctaccggc cgcaactcgt gtccagcatc
      541 cagaacgccg agggggaggt gctgagttcg ttctccccgg tggttcaagg taccctcccg
      601 gttagtgcgg agaatctggc cgccattcaa caagccatga tcagagtgat ccgggatccg
      661 cgggccacgg cctaccggcc gttcctcgga atacccgata tcaatattgc gggcaagacc
      721 ggcaccgccg agaccgggga gttcaccgat acccatgcct ggtttggggg gtacaccttc
      781 gaagagcgct cagacagacc agatatcgcg gtggtggtcg tgctggagta tcagggccaa
      841 ggctccgagt gggcagcgcc ggtattcaag cgagtggtcg aggcctactt caaggggggt
      901 ccgatcagcc gctatccctg ggaagctcgg attcgagtcc cggcgactcc cgagccggac
      961 ccagaagaac agggaaccga gggatagggc tggggaccat cgcgaagccg ctacacggct
     1021 tgatcctgcg cgcccgggcc ggtttctatg acgttgagac tgagcagggc gtggtcacgg
     1081 cgaagctgcg cgggcgattg aaaaagttgg ccccccgagc cgaactggca gcgatcgggg
     1141 atcgggttaa gctcagcctg tttgaggacg gctcggcctc gatcgaagag atcctgccgc
     1201 gccaacgggc gctttcccgt cgggctcccg gccgggagat cgaacagatc ctggtggcca
     1261 acccggatca agcggtattc gttttcgcct gtgccgatcc ggaccctaat ttccgcatgc
     1321 tcgaccggat gctagtgggt gccgaacggg aaaccatccc ggcggtgatc tgcgccaaca
     1381 agatcgatct ggtgcaaccg cgcagcgccc gagaggaatt tgacgtttac gagaacattg
     1441 gataccgagt gctctacacc tctgcgacca cgggaaaagg tgtgggagac ttgcgccggg
     1501 aactgagcgg gaagctctcg gtgttggccg gaccatccgg ggtgggcaag tccagcctgc
     1561 tcaacatggt tcagccgggt cttggacttc gcaccgacgc cgtgagccaa gctaccggca
     1621 aggggatcca caccaccgtg gcccgggagc tgctcccgct cactggaggc ggatatgtcg
     1681 ccgatacccc cggcctcaag gcgttcgccc tctgggatat cgagccggac gaactggacg
     1741 cctacttccg agagatcgcg ccgttggtgg agggttgcga attcagcgac tgcacccacg
     1801 agcatgagcc cggctgcgcg gtgcgggccg cggtggaggc aggcacgatc gacgttaatc
     1861 gctacgagtc ttaccttcga atgcgatcgg gcgagccctt catagagcgc taactcggcg
     1921 cggcggccgg cagcgccgtg gctagctgct ttcgctcacc aggaaggcaa tcagggcttc
     1981 aatctcctcg gcagaaagcc gcaagttcgg catttgagtt cccggcctca ccgcgggcgg
     2041 atccttgagc cagctgcgaa ggaaatccgg atcgtaggac cgagagctca gctctggtcc
     2101 gacggagacg ccggatcctg ggttcaccgc gttgtgctga tgacaaccca cgcacccttt
     2161 ggcctggaag agggccctcc cgtacgccgc aggatcgtca gtggccgccg ccgagaccgc
     2221 gggtggcggt gttgggccgc cacccgggtt ttctgaggcg ggaaccaggg acaggagcag
     2281 caacccagta cccgccagcg ccaacgacag gactcctcgg ttgatcacga tttgccgcag
     2341 cggctcaggc ccgctcttca actgtctggc gcgcgccgcg ccggccgagg acggccagtg
     2401 cccctgcgcc taacaacaga gcgattccgg accagcggag ccatccgcgc aggtcgctgg
     2461 ctccgagtgt gacggtttgc tcggggctgg cggccggctg ggtcaaggta gccggcagca
     2521 cgctcagggg ctcgagttgg gtcccctcga acggcgcggg aacgattgac cattcccagg
     2581 tgcctgccgc cgggaagacg atctcgacga cgaaatgccc tacttcgcct tcctgaaccg
     2641 cgtacgcccg cacactctca cccgactcgg gattgtaggc cagcacatac gggtcaacct
     2701 cgttggtcgg ggtctttccg tgctggcgaa ccatgaaccc caggctcacg ctctcaccgg
     2761 cctggacttc gcccggcaat tgatctagcg tggcgatggc ccatccgcct gccgccgcgg
     2821 caggaacgag tgcgaaggcc aatgcgagca caatcaagga accaagtaca cgacgagaca
     2881 tggcgttcct ccttgggtga agtacccatt tgaagctaga tacaccggcc ggcccgggaa
     2941 ggttcccatg aggctcagtt gaccgggttg tgccaagcga agctgaatga agaccgcgaa
     3001 gatcagaggg taagcaacta catcgggcgc agcacccgat cttcccgaag gcgcttgaac
     3061 cacagcgagg acccgcgcgg catcgactcc accgcggctt cctgccaggc ccggacgccc
//
LOCUS       DATDPQ010000170         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_855029, whole genome shotgun sequence.
ACCESSION   DATDPQ010000170 DATDPQ010000000
VERSION     DATDPQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855029"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VI520_03995"
     CDS             <1..319
                     /locus_tag="VI520_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37079.1"
                     /translation="TMMHVNAPHPNCAYKWLEHSINPKLQGDLASWFGSVPAVLSACT
                     GNALLTDEGCDINGLGNFEQIAFWKTPAAACGFDPAGDAGPNDCVPYHEWVTNMIAVI
                     GGR"
     gene            419..1864
                     /locus_tag="VI520_04000"
     CDS             419..1864
                     /locus_tag="VI520_04000"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HLF37080.1"
                     /translation="MNHQLWINGKWEPSKGGGTMAILNPATEEKIAEVVDSSREDVDR
                     AVQAAKTAFYDGRWSKRTPSERSKILWKLADLLEARMADFARAESENTGKPYNFVSLG
                     ADLPFTVDNLRFFASAARDMHGSHAGEYLAGYTSIFRREPVGVVGQITPWNYPLMMAV
                     WKIGPALAAGCTAVLKPAPTTPLTTLMLAELSKEAGLPDGAFNIVTGGNESGQALVEH
                     PDVRMVSLTGSPRTGMRVMETASKTLKRVHLELGGKAPLLVFEDADADLVVSKVTLAA
                     TFNTGQDCTAATRVYVEKGRYKPLQEALVESMRAVKLGEPFAEGVAMGPLISAVQRER
                     VQGFVDRAKAGGARVLTGGGIPKGTSKGFYFEPTVVVEAGQQSEIIQSEVFGPVLTIS
                     PFNDAEEALTLANDVPYGLSASIFTKDIGRAMKLSANIEAGTVWVNDHIPLTSETPHG
                     GFKHSGFGKDLSAEAMGDYQITKHVMVSHTG"
     gene            1861..>2433
                     /locus_tag="VI520_04005"
     CDS             1861..>2433
                     /locus_tag="VI520_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF37081.1"
                     /translation="MSSGSPPAIRLDRVSRQFGDVKAVDDVSLDIPDGEFFTLLGPSG
                     SGKTTSLRLIAGFDHPSAGRILLHGEDVTSLPPYEREVNTVFQDYALFPHMSVGENIG
                     YGLMIRSLPKPERARQVAEMMELVRMPGLEDRRPSQLSGGQRQRVALARALILRPRVL
                     LLDEPLGALDLKLRQQMQVELKSIQEQVGIT"
BASE COUNT          457 a          752 c          814 g          410 t
ORIGIN      
        1 cacgatgatg cacgtcaacg cgcctcatcc gaactgcgcc tacaaatggc tggagcactc
       61 gatcaatccc aaactgcagg gcgatctggc ctcctggttc ggatcggtcc cggcggtgct
      121 ctcggcctgc acaggcaacg ccctgctcac cgacgagggc tgcgacatca atggcctggg
      181 caatttcgag cagattgcct tctggaagac cccggctgcg gcctgcggtt ttgatccggc
      241 cggagacgcg ggtccgaacg actgcgtacc gtatcacgag tgggtcacga acatgatcgc
      301 ggtgatcggg ggtcgttgat ctcccgaccc tgacttgggg ggcggacttc gccaccagcc
      361 tctccccacc catgggtggg gagggagtga gggcgaggtc gttgaagccg gaggacgaat
      421 gaaccatcaa ctgtggatca acgggaagtg ggagccgagc aaaggcgggg gaacgatggc
      481 gatcctcaat cccgcgaccg aagaaaagat cgcggaagtg gtcgatagca gtcgggagga
      541 cgtcgatcgg gccgttcagg ccgcaaaaac tgccttctac gacggccgct ggtcgaaacg
      601 gactccgagc gagcggtcca agatcctgtg gaagctggcc gacctgttgg aagccaggat
      661 ggccgatttc gcccgcgctg agtcggagaa caccggaaag ccctacaact tcgtcagcct
      721 gggcgccgac ctgcccttca cggtcgacaa cctgcgcttc ttcgcctcgg ccgcccggga
      781 tatgcacggt tcgcatgccg gcgagtatct cgccggttac acctcgatct tccgccgcga
      841 accggtcggg gtggtcggcc agatcacccc gtggaactat ccgctgatga tggcggtctg
      901 gaagatcggc ccggcgctcg ccgcgggctg caccgccgtg ctaaaaccgg ccccgacgac
      961 tccgctcacc acgttgatgc tcgccgagct ctccaaggaa gcggggctgc cggatggggc
     1021 cttcaatatc gtcacgggtg gcaatgagag cggtcaggcg ctggttgagc accccgacgt
     1081 gcggatggtg agcctcaccg gctcgccgcg gaccggcatg cgggtgatgg agaccgcctc
     1141 aaagaccctg aaacgggtgc accttgaact ggggggcaag gctccgctgc tggtgttcga
     1201 ggacgcggat gccgacctgg tggtatccaa ggtgaccctg gccgcgacct tcaacaccgg
     1261 gcaggactgc acggcagcca cccgagtgta cgtggagaag ggccgctaca agccgttgca
     1321 ggaggccctg gtcgagagta tgcgagccgt gaagctgggt gagccgttcg cagagggcgt
     1381 ggcgatgggc ccgctgattt cggcggtcca gcgcgagcgg gtgcagggct ttgtcgaccg
     1441 ggccaaggcc ggcggcgccc gggttttgac cggcggggga atccccaagg ggacttccaa
     1501 gggcttctac ttcgagccga ccgtcgtggt cgaggccggg cagcagtcgg agatcatcca
     1561 gagcgaagtg ttcgggccgg tgctgaccat cagcccgttc aatgacgcgg aggaagcgct
     1621 tactctggcc aacgacgtgc cctatgggtt atcggcctcg attttcacca aggacatcgg
     1681 gcgggccatg aagctctcgg ccaacatcga ggcgggcacg gtctgggtca acgaccacat
     1741 tccgctcacg tccgagacgc ctcatggcgg cttcaagcac tccgggtttg gaaaagacct
     1801 gtcggccgag gcgatggggg actaccaaat caccaagcac gtcatggtca gccacaccgg
     1861 atgagctccg gctctccgcc ggcgatccgg ctggaccggg tcagccgcca attcggagac
     1921 gtcaaagccg tcgacgacgt cagcctggac atcccggacg gcgagttttt cacgctgttg
     1981 ggcccctccg gttcaggcaa aacgacctcc ctgcggctaa ttgccgggtt cgaccatcct
     2041 agcgccggcc ggatactgct gcacggggaa gacgtgacca gccttccacc ttatgagcgg
     2101 gaggtcaaca cggtcttcca ggactacgcc ctctttcccc acatgtcggt gggcgagaat
     2161 atcggctacg ggttgatgat ccgcagcctc cccaagccgg agcgtgccag gcaggttgca
     2221 gaaatgatgg agctggtccg aatgcctggc ctggaggatc gccggccgtc ccaattatct
     2281 ggcggacaac ggcagcgggt ggcgctggct cgggccctca ttctgcgacc gcgggtgctg
     2341 ctgctggacg aacccctggg ggcactggac ctcaagctgc gccagcagat gcaggtcgag
     2401 ctgaaatcca tccaggaaca ggtggggatc acc
//
LOCUS       DATDPQ010000171         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1130005, whole genome shotgun sequence.
ACCESSION   DATDPQ010000171 DATDPQ010000000
VERSION     DATDPQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130005"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VI520_04010"
     CDS             complement(<1..188)
                     /locus_tag="VI520_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37082.1"
                     /translation="MKLVECVPNFSEGRRIDVIEAIENAIRSVEGIQVLDRHVDADHN
                     RSVITFVGSPAAAAEAAFA"
     gene            462..695
                     /locus_tag="VI520_04015"
     CDS             462..695
                     /locus_tag="VI520_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37083.1"
                     /translation="MSEKKTKLLNLMRFWLFGTFVIVWAAVTGYIGLFTDRDWWVAIK
                     AGFPIWGITGVLAIVWYFFYKWWLGRKEDSPTA"
     gene            complement(765..1946)
                     /locus_tag="VI520_04020"
     CDS             complement(765..1946)
                     /locus_tag="VI520_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF37084.1"
                     /translation="MSAADSGEGALNFELSEEQRMWQAAVHEFCAAEVKPLAAEMDAR
                     SEFNSGAVQKMASLGLLGLNVPEELGGAGVDPISAAIAIEELGWACGGTALSVSAHNG
                     LVCAPIALFGSEDQQRRWLPRLARGEGGLGALALTEPGGGSDLQGAIQTRAEYAGGEW
                     VIRGSKAWITNASVASLIVVLCRTDPAGGSRSLSLIVVPTEAEGLHIHPAEKKMGVRA
                     SPTHALTLERVRVPGDHLLGTAGDGLTQALQVLDGGRVGIGALAVGLAQAAFEEAVGY
                     AKQREAFGTRLSGHQAIQAMIADAGTELESARLLVYRAAWLKGQGIPFTREAAMAKLF
                     ATEMAERVCRNAIQILGAYGYSSEYPVERLYRDARLMTIGEGTSEIQRLVIARRTLGL
                     E"
     gene            complement(1947..>2914)
                     /locus_tag="VI520_04025"
     CDS             complement(1947..>2914)
                     /locus_tag="VI520_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLF37085.1"
                     /translation="LGATFILYDGAPDYPGPDRVWAMVEQHGVTSLGLSPTFVRSLIP
                     HGEQPIERHDLSSLLCFGSTGEPWNPDPWMWLFEKAGRSSRPIINYSGGTEISGGILM
                     GNPILPLKACAFSGPCPGIAADVFDENGNSVRNQVGELVIKTPWIGMTRGFWKDPARY
                     LQTYWSRWENVWVHGDFAAVDSDGLWYILGRSDDTIKVAGKRVGPAEVESILVEHPSV
                     VEAAAVGLPHPVKGNELIAFCVLTAASAGTPGLAEELRAKVGRELGKPLTPREVRFVH
                     DLPKTRNAKVMRRMIRAAYLGEDLGDTSALVNPESLAEFTPSRPA"
BASE COUNT          528 a          949 c          892 g          545 t
ORIGIN      
        1 gcaaaggccg cctcggccgc ggccgccgga gatccgacga acgtaatgac gctgcgattg
       61 tgatcggcat cgacgtgccg gtccaacacc tggattcctt cgaccgagcg gatcgcgttt
      121 tcgatggcct cgatgacatc gattcggcgg ccttcggaga agtttggaac acactcgacc
      181 agtttcattt cagtctcgcc ggctccgtcg gttcaggccc ggctgattat agccagttgg
      241 caagggcgga taagcgatca gtagtctcct ggccgtacaa ttcggtgaac ggtttgccgc
      301 cgattggcgc ccgtcatgca cggcctcgag ctctgctggc ggccatcggc tagatcgagc
      361 gctcaatcgg accgacaccg tggctagccc tgggagccgc gtttgcgcgt tttgggcagg
      421 tggctataat gccgcctcgc cactagacaa gggaggatga cgtgtcggag aagaagacca
      481 aactactgaa cctgatgcgc ttctggcttt ttgggacgtt tgtgatcgtg tgggccgcgg
      541 tgacgggcta catcggactc tttaccgatc gtgactggtg ggtggcaatc aaggccgggt
      601 tcccgatctg gggcatcacc ggggtgctgg cgatcgtgtg gtacttcttc tacaagtggt
      661 ggctgggtcg gaaagaagac tcaccaaccg cctgaatcaa caatcgaacc aggattgacg
      721 cgcctgcatc acggcaggtg cgtttgtttc cagcgcgagc gcggctactc gagcccgaga
      781 gtgcgccggg cgatgaccaa gcgctggatt tcgctggttc cttccccgat ggtcatcagg
      841 cgcgcgtcgc ggtaaaggcg ttccaccggg tactcactgc tgtatccata ggcacccagg
      901 atctggattg cattgcggca aacccgttcg gccatctcgg tggcgaacag cttggccatc
      961 gcggcctccc gggtgaaggg gatcccttga cctttcagcc aggccgcccg gtacaccagc
     1021 agccgggcgg attcaagctc ggtccccgcg tcggcgatca tcgcctggat cgcttggtgt
     1081 ccgctgagcc gggttccaaa ggcttcgcgt tgtttggcgt agcctaccgc ttcttcgaac
     1141 gcggcctggg ctaagccgac cgctaacgcc ccgatcccaa cccgacctcc atccagtact
     1201 tgcagcgcct gagtcagtcc atctccggcg gtccctagga gatggtcgcc gggtacccgc
     1261 acccgttcga gggtcagggc gtgggtcggc gaagcccgaa cccccatctt tttctcggcc
     1321 gggtggatgt gcaggccctc agcctcggtc ggcacgacga tcaagctgag cgaacgggat
     1381 ccgcccgctg gatcggtccg gcataagacc acgatcaggg aagccacgct ggcgttcgta
     1441 atccaggcct tgctgccccg gatcacccat tcgccgcccg cgtactcggc acgggtttgt
     1501 atggcgccct gcagatccga ccctcccccc ggctccgtca gcgccagcgc tccgagaccg
     1561 ccttcgccgc gggccagcct gggcaaccag cgtcgctgct ggtcctccga tccgaacaag
     1621 gcgatcgggg cgcagaccag cccattgtga gcggaaactg acagcgccgt gccgccacag
     1681 gcccaaccga gttcttcgat ggcgatggcg gcgctgatgg ggtcgacccc agccccgccg
     1741 agctcttccg gcacgttcag gcccagtaac ccgagggagg ccatcttctg cacggccccc
     1801 gagttgaatt cggaccgggc gtccatctct gccgccagcg gcttgacctc ggccgcgcag
     1861 aattcgtgaa ccgccgcctg ccacattcgc tgctcttcgc tcagctcgaa gttcaaagcg
     1921 ccctcaccgg aatcggccgc cgacattcat gccggacggg atggggtgaa ttctgccagc
     1981 gattcagggt tgaccaatgc ggaagtgtcc ccgagatctt cccccaagta agctgcccgg
     2041 atcattcggc gcatgacttt ggcgttgcgg gtcttcggga ggtcgtgtac gaaacggacc
     2101 tcgcgcggag taaggggctt gcccaattcg cgccccacct tggcccgcag ctcctcggcc
     2161 aacccaggcg taccggcgga cgccgcggtg agcacacaga aggcaatcaa ttcgttgcct
     2221 ttaaccggat ggggaagacc caccgcggcc gcctcgacca ccgacggatg ctcaaccaga
     2281 atcgactcga cctccgccgg gcccacccgc ttgccggcca ctttgatcgt atcatccgag
     2341 cggcccagga tataccacag accgtcggaa tctaccgccg cgaagtctcc atgcacccag
     2401 acattctccc acctcgacca ataggtctgc aggtagcgcg ccggatcctt ccagaagccg
     2461 cgggtcatcc cgatccaggg ggtcttgatc acgagctctc caacctgatt gcgaaccgag
     2521 ttcccattct cgtcgaagac atcggcagcg atgccggggc aggggccaga aaaggcacag
     2581 gccttcaaag gcaggatcgg gttgcccatc aggatgccgc ccgaaatctc cgtgcctccg
     2641 gagtagttga tgatcggacg gctgctccga cctgcttttt cgaagagcca catccacgga
     2701 tcgggattcc acggctcgcc agtcgatccg aagcatagca gcgaggacag gtcgtggcgt
     2761 tcgatcggct gctcaccgtg tgggatcagc gagcggacga aggtcggtga tagtccgagg
     2821 ctcgtgacgc cgtgctgctc aaccattgcc caaactcggt cgggaccggg gtaatccgga
     2881 gccccgtcgt ataggatgaa agtcgccccg agca
//
LOCUS       DATDPQ010000172         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3746920, whole genome shotgun sequence.
ACCESSION   DATDPQ010000172 DATDPQ010000000
VERSION     DATDPQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746920"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VI520_04030"
     CDS             <1..350
                     /locus_tag="VI520_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLF37086.1"
                     /translation="ELSFQLDAELSAWLRWVAQGRQGSPQALAAELLTRGLEQEARRA
                     RAHGLLQLLTPRERQVTQLTGQGQTNHQIAHALKISPETVKSHVRNTLSKFGLHSKAD
                     LRVLLKELGEADS"
     gene            404..1447
                     /locus_tag="VI520_04035"
     CDS             404..1447
                     /locus_tag="VI520_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLF37087.1"
                     /translation="MEHRLLGPTGVRVSPLCLGTMNFGGPTAEPESIRMIHQALEAGI
                     NFLDSANVYNRGESERVVGRALDGARRAKVVLATKVHFPMSNDPNDRGNSRRHILQAV
                     EDSLRRLNTEWIDLYQIHRPVLDIPQQETLHALDDLVRAGKVRYIGCSTFPAWMVMEA
                     LAISERDGLVRYVTEQPPYNLLDRRIENELIPLAQRYGLGILPWSPLAMGMLASRYLQ
                     ADEHPPDSRAARVGSYAAARISEAGVAAGRQLAELALARGLTPSQLALLWVKDQPAVT
                     APIIGPRTEAQLQDALAVLEMRLDGQTASALDQIVPPGSAVADFHNTSGWMKMRVTSI
                     APLPSLDEMYLPR"
     gene            complement(1560..>2313)
                     /locus_tag="VI520_04040"
     CDS             complement(1560..>2313)
                     /locus_tag="VI520_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37088.1"
                     /translation="GSQLGRRGFFTDMVRIADLVAVPAISEGVANQYSEGCFATWDPK
                     LGALIATVTGSARPVDKDAIGEDDLAVIVGVRPDGKGALVRHVAGKRNDPPSSEAVEL
                     MEMDSLLPKVRLEAGWKTQAAVPVLRSKLHGHRGVAAYDPARVEHVRLEPAYYHYPVS
                     CATEAQAWAIKDAFGRSAALRDDADPRKLVFTVLPGHGVVIAEKWLAGKAPFQAMWEA
                     MDAGELEISNRVPQAMLGYVPGPNGRMILQEG"
BASE COUNT          404 a          793 c          720 g          396 t
ORIGIN      
        1 tggaattgag cttccagctc gacgccgaac tctcggcctg gctgcggtgg gtcgcccaag
       61 gccgccaggg ttcgccgcag gcgttggccg ccgagctgct gactcggggc ctggaacaag
      121 aggcccgccg ggcgcgggcc cacggcctgc tccagctgct cacgccgcgc gagcgtcaag
      181 tcacccagct taccggtcag ggccagacta accaccagat cgcccatgcg ctgaagattt
      241 caccggagac cgtcaagagc catgtgcgca acacgctgag caagttcggc ctgcacagca
      301 aggccgacct gcgggtgctg ctgaaagagt tgggtgaggc ggacagttga cctgctgccg
      361 caagtgcagg taagctcagg acgaagaccc gaggggagcc gctatggaac atcgcttgct
      421 ggggcccacc ggcgtgcgcg tctcaccgct ctgcctggga accatgaact tcggcggacc
      481 caccgccgag cccgagtcga tccggatgat ccaccaggcg ttggaagccg ggatcaattt
      541 cctggatagc gccaacgtct acaaccgcgg cgaaagtgaa cgggtggtgg gtagggccct
      601 ggatggcgcg cgtcgggcga aggtcgtcct ggccaccaag gtgcactttc ccatgtcgaa
      661 tgacccgaac gatcgcggaa actcgcgccg gcacatcctg caggcggtag aggattcgct
      721 ccgccggctg aacactgagt ggatcgacct ctaccagatt catcggccag ttctagatat
      781 ccctcagcag gagaccctcc acgccctgga cgacctggtg cgggccggca aagttcgcta
      841 catcggctgt tccaccttcc cggcctggat ggtgatggaa gctctggcga tcagtgaacg
      901 cgacggcctg gtccgctacg tcaccgagca gcccccctac aacttgctgg atcgccgaat
      961 cgagaacgaa ctgatcccac tggcccagcg ctacggactg gggatcctgc cctggtcgcc
     1021 cctggccatg ggaatgctgg cgagtcgtta cctgcaggcc gatgagcatc cgccggactc
     1081 ccgggctgcc cgggtaggct cctatgccgc cgcccgtatc agcgaggccg gggtggccgc
     1141 cggccggcag cttgccgagc tggctctcgc ccgcggcctg actccctccc aattggcctt
     1201 gctctgggtc aaagatcagc ccgcggtgac cgcaccgatc atcgggccgc gcaccgaagc
     1261 ccagctgcag gacgccctgg ccgtgctcga aatgcggctc gacggtcaga ccgcctccgc
     1321 gctggaccag atcgtgccgc ccggttcagc ggtcgccgac tttcacaaca ccagcgggtg
     1381 gatgaagatg agggtcacct cgatcgcgcc gctcccatcc ttggacgaga tgtacctgcc
     1441 gcgctgagcc gcggaaaaga ttgtgctcgt tcgtggctgg cccgaagcta gcggcgctcg
     1501 gactcggtgc aggtgcgtag gggacttgct gtcttaacta gcttccggtc tgcggaagtt
     1561 cagccttctt gaaggatcat gcgcccgttt ggtccaggca catagccaag cattgcttgc
     1621 ggaactcggt tcgagatctc caactctcca gcgtccatcg cctcccacat cgcctggaag
     1681 ggcgccttgc ccgccagcca cttttcggcg atcaccaccc cgtggccggg caacacggtg
     1741 aataccagct tccgcgggtc ggcatcgtcg cgtagcgcgg ctgatcggcc gaacgcgtcc
     1801 ttaatcgccc acgcctgcgc ctcggtggcg cacgacaccg ggtagtgata gtaggccggc
     1861 tccaaccgga catgctcgac ccgagccgga tcgtaggctg caacgccccg gtgcccatgc
     1921 agtttggagc gcaacacggg aaccgcggct tgcgtcttcc accccgcctc gagcctaact
     1981 ttcggaagta ggctgtccat ctccatcaac tctaccgctt cggaagaagg cgggtcattg
     2041 cgcttcccgg caacgtgccg caccagggcg cctttgccgt ccggccgcac gccgacgatc
     2101 actgccaggt cgtcctcccc gatggcgtct ttgtcgaccg gccgggcgct gcccgtcacg
     2161 gtcgcgatca gggcgcccag cttcggatcc caggtcgcga agcagccttc actgtactga
     2221 ttggccactc cttccgaaat ggccgggacc gcgaccaggt ccgcaatccg gaccatatcg
     2281 gtgaagaacc cccgccggcc aagctgcgat ccg
//
LOCUS       DATDPQ010000173        10432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3746969, whole genome shotgun sequence.
ACCESSION   DATDPQ010000173 DATDPQ010000000
VERSION     DATDPQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746969"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VI520_04045"
     CDS             complement(<1..315)
                     /locus_tag="VI520_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLF37089.1"
                     /translation="MQYLLVMYGDQKAQANLTPEQMAATHQEYVRFDESLRKSIKVLS
                     NIGLAPTSAATTVRFKNGKVVTSDGPFAETKEQLFGYYLIEAKDLDEAILWAGKMPNL
                     PGG"
     gene            501..1475
                     /locus_tag="VI520_04050"
     CDS             501..1475
                     /locus_tag="VI520_04050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLF37090.1"
                     /translation="MTTDFKGYLLSEFLGDYEQGLLTRREAGKLIAGIVGSLTLAESL
                     LAACTPAGTNGSSGSLTPSSAASESPGASSSGPTASSTGAELPTASESGSPAAGEVQI
                     PGTDTPLLAYLARPAGDGPHPAVLVCHENRGLTEHIKDVARRLASAGYVGLAVDLLSR
                     QGGTAAVADSSQIPGMLSSQPPEQFVRDFTDGWRWLQTQPEVRPDGVGMVGFCFGGGV
                     TWRVAIGLADLRAAVPYYGPHPDPNELAAIQGAVLAIYAGRDDRINRSIPAIEAEMQR
                     LGKVYEKVIYPDTDHAFHNDTGSRYDPAAATDAWSRTLAWFDRYLRSS"
     gene            1594..3432
                     /locus_tag="VI520_04055"
     CDS             1594..3432
                     /locus_tag="VI520_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLF37091.1"
                     /translation="MLSTLRHLIVGSPLTTQQLSSERVNKVRALAAFSPDALSSIAYA
                     NQEIFLGLVVAGSAGLALSWPIGLAITGLLILVAFSYFQTVHAYPSGGGSYIVARENL
                     GTLPGLVAAAALLIDYVLTAAVSLTAGVAAIASAFPGLWPHRVTLALLGLLLITLLNM
                     RGVRETGTVIAIPVYLFLATYLPMLAYGLVRALIEGPGSFHAAALPTLQPLTALLILH
                     TFSAGCTALTGIEAISNGVPAFRPPESRNAGITLIVMAVLMAVLFAGSIGLTQYLAVV
                     PGPEETILSALARQLLGSGLGYVTIQVSTLLILVVAANTSFAGFPRLASILARDGFLP
                     RQLSALGDRLAFNNGILVLTAASAALIVIFQGDTHALIPLFAVGVFLAFTLSQVGMVV
                     HWAQERKGSWAVKAGINGVGGLLTGMTLVIVGVSKFVEGAWISLLLIPLLVGIFLPIR
                     AHYRELARQLSLQGSSPSLQKFAPIRAVVPISGVHRGVVDAINFARAITKNVTAAYIE
                     LEPGSGEKVQQEWARSWPDVPLVIIPSPYRSVIGPLLDYLDLIDTRYNDGQAAVVVLP
                     EFIPAKWWQSLLHNQTAWLIKVALLYRRRRKGFQRVIIDVPYHTAR"
     gene            3501..4430
                     /locus_tag="VI520_04060"
     CDS             3501..4430
                     /locus_tag="VI520_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HLF37092.1"
                     /translation="MITIYKNGVQGLQPAEAVENGCWVHAVAPTEQDLAVLAGHGVPP
                     EFLSHLLDLNERPRAEREGDAILLVLHFPYAQGPIADIPYITVPLTIIVTTDLVVTSV
                     PMATGFLQGFASRNLRGMTPDRPTGFVLQMLLYLAKEYLDNLNVINAQVDRLEDQLQR
                     SLRNQEVLGLLKFEKSLTYFTTALRSNEVVLGRLQRGNLSQWHPDEMELLEDVLIELR
                     QAIEQVDISQSILAQMMDAFASIVSNNLNSVLKLLTSVTLLVSLPSLIASVYGMNVIL
                     PGAESPLAFAGILGASILVSLGLLVIFWRKDWL"
     gene            complement(4441..6675)
                     /locus_tag="VI520_04065"
     CDS             complement(4441..6675)
                     /locus_tag="VI520_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37093.1"
                     /translation="MLSLILLLLIVFLAFRLAPRERRETAQVHGAGQIGLLAALALFG
                     VDYFTSYYYATGEMMSALHPYGLEDRAYLAVAVIAFANFAFGLLYMFSLGPFNEGGGS
                     YTASMRYLWPTLSLIVAVALIQDYVLTIVVSALSGGDQLLSIIGLYGKNWLYHFGLGA
                     VLAAITWYLTIRGRGESAQIVFSLITVFALLTIAMAIGLIMAHFRGVPPVPPEAAPRA
                     VTLGQAMLHMLTAAMKGMVALTGLEAVSNGIQFMQDEDAGIVKWGKKRLSRLSWLWNF
                     YSGKSGIGRFVQTSFLFYGGLTTFFLTVFAIRFDVFDGTFGRTLVGNLAFIAFGEIPG
                     GILLFWAYQIVAVMMLAAASMTALQDAQATEWRDVAMGGIPEAIIYRDRRGTFTRSVT
                     ITFAMAVLIMFLVRGRTTVAVPFYGVGVFMPITAMGLAVRKHLLTHYTGRARRWGAAG
                     ATFVTVLAGIVFLGQLIGKWEEGGWIALLGFGFLAIVAHVVLLSPFGYREPRQIYRIV
                     RTKARVQGAMASIVEWQAFRMQEYRYRLMIAIASFLELFGVGQQARRLPAFALANGGR
                     ILPKPSATRAEPRQANPPTTPAHRVKTPAPVADEPAPPRITRHRIILPINAVHQGTLT
                     ALRYATSLSNDVTAVHVALDQADADTLRNEWDRRGDGIRLVVLPADHGMVLEPLLKYI
                     VEMMELRQPHESMTIVVPQSVRPRWWSNLMRTQMASLLRLSLPLETGVVITDVPYQLD
                     GDRD"
     gene            complement(6710..6895)
                     /locus_tag="VI520_04070"
     CDS             complement(6710..6895)
                     /locus_tag="VI520_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37094.1"
                     /translation="MLRVLDWMIPFGLVLGFFWLQVRLQPLASGRFHDVLMAISIIVE
                     IRLIWLWERWSTRRVQA"
     gene            complement(7029..7727)
                     /locus_tag="VI520_04075"
     CDS             complement(7029..7727)
                     /locus_tag="VI520_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF37095.1"
                     /translation="MTSRPKVLVIDDEPQILRALRTILGEKRFEVTTATRGEEGLARA
                     AADPPDVIILDLGLPDMDGLTVIERVREWTQIPIIVLSVREADADKVEALDKGGDDYL
                     TKPFSIEELLARIRVALRHDARMQGSLGETVVTAGPISIDLALHTVRRDGQEVKLTAT
                     EFRLLAYLAANAGRVLTHRGILTSVWGPTEADHVEYLRVYVRQLRKKLEPDPERPQFV
                     LTEPGVGYRFLADD"
     gene            complement(7724..9190)
                     /locus_tag="VI520_04080"
     CDS             complement(7724..9190)
                     /locus_tag="VI520_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37096.1"
                     /translation="MPGVSLTARVASRYVFATLAIGLTTALLYLWRSNLGTPLIALLY
                     LLPVGLSAAYWGLGPGVLTAVWTFLAFNYFFIEPRFTLVVHRTEDTLVLLVFLVVAVV
                     ISQLVGRAQAGLAAANAREREATRLYELSTALAGSLDVSAIGRTLCEQVKSTFQADRV
                     QLAAAGGGHFPAPEVSLPAEAMTTPTPASLISVLQTARGQLGEIRLWRGSAPISTSED
                     RLLRTFASQGALALERAWLAEAEQRARVLEESDRLKSAVLSSVSHELRTPLATIKAAA
                     TSLRSGEVAWDSAARADLLGAIDDESDHLNRLVGNLLDMSRIEAGALRPSRQWNLLQE
                     IVGGVVERMRPLTGQHRLELDIPEELPLVPVDYVQIEQVFTNLVSNCLKYAPPHTRIQ
                     VSARPTGDGSLQVQVSNQGPPVPERDLARIFDKFYRITAADRVTGTGLGLSICKGIVE
                     AHGGRIWAENIPIGFAFNFTLPMTWDGNPPPNLPPEAE"
     gene            9287..9847
                     /locus_tag="VI520_04085"
     CDS             9287..9847
                     /locus_tag="VI520_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37097.1"
                     /translation="MTTNAAKGRVAGISDEAVRAKTGKSWSEWIDVLDSAGAADMDHP
                     AIAKYLYNDLGVPDWWSQMVTVGYEQARGRRRVHETARGFQISRSRTMEVSAARAFKA
                     WSEAAVRNRWLPGRSLKVRKATHNESIRSTWSEDGSDVSVDFYGKGPLKVQVVVQHSG
                     LSDAKQAERMKEFWTAALVRLKTQLE"
     gene            9922..10251
                     /locus_tag="VI520_04090"
     CDS             9922..10251
                     /locus_tag="VI520_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HLF37098.1"
                     /translation="MAYDKKLADRIRKALSKQPKLTERQMFGGIGFMLRGNMACGVIG
                     DEMMVRIAKEETDSAIKEPGARIFDFSGRPSPGWILVNQQVLSKPAELRAWVNRGVEY
                     ALSLPAK"
BASE COUNT         2004 a         3231 c         3230 g         1967 t
ORIGIN      
        1 gcccccgggt aggttgggca tcttgccggc ccagaggatc gcctcgtcga gatccttggc
       61 ctcgatcaag tagtacccga agagctgctc tttggtttcg gcgaatggtc catccgaagt
      121 gacgaccttt ccattcttga accgcacagt cgtggcggcc gacgtcggcg ccagcccgat
      181 gttgctcagg accttaatgg attttcgtag cgattcatca aacctgacgt actcctgatg
      241 agtggccgcc atctgctcag gggtgaggtt cgcctgcgcc ttctgatccc catacattac
      301 gagcaagtac tgcatttcat tctcctttgg tggatggttc cagcgctcct ggctggtttc
      361 attcatacga cgaacgggta tcggccaatt cgacagatcc ccatggtaac ccgatggccg
      421 gctggctctg tccgaactgc aatcctcagg cgacagggta tcatggtctg gagcccggcg
      481 tcccgctcgg aggatcaaga gtgacgaccg atttcaaagg ttacctgctg agcgaattcc
      541 tgggggacta cgagcagggt ctgctgaccc ggcgagaggc cggcaagttg attgcgggaa
      601 ttgtcgggag ccttaccctg gccgagtcgc tgctggccgc ctgtacgccg gcaggtacga
      661 acggcagctc tggttcgctc accccttcgt ccgcagccag cgagagccca ggcgcctcat
      721 cgtccggacc gaccgcatca tccactggcg ctgagctacc gaccgcgtct gagagtggct
      781 cccctgcggc cggcgaggtt cagatccccg gaactgacac acccttgctc gcctacctgg
      841 cccgcccggc aggagacggt ccgcacccgg cagtcctggt ctgccacgag aatcgcggcc
      901 tgaccgagca tatcaaggat gtcgcccgcc ggctggcgtc cgccgggtac gtcgggctgg
      961 ccgtcgatct gctttcgcgg cagggcggga ccgcggccgt cgccgattcc tcgcagatcc
     1021 ccgggatgct cagcagtcaa ccgcccgaac agtttgtgag agatttcaca gatggctggc
     1081 gctggctgca aacccagccc gaagttcggc cggatggagt cgggatggtc gggttctgtt
     1141 ttggcggcgg ggtcacctgg cgggtcgcga ttgggctagc cgatttgcgg gcggccgttc
     1201 catattacgg tccgcatccc gaccccaatg agttggcggc gatccagggc gcggtactgg
     1261 cgatttacgc cggccgagac gatcggatca accggagcat cccggcgatc gaggccgaga
     1321 tgcagcggct tggcaaagtc tacgagaaag tgatatatcc cgacaccgat cacgctttcc
     1381 acaacgacac cggctcgcgg tacgaccctg ccgcggctac cgacgcctgg tcacgaacct
     1441 tagcttggtt cgatcgatat cttcgcagtt catgaccgag agggctcagg attggtcccc
     1501 aaaggagttg tgcatcgaac tcgggcaccc gagcatgcgg tcgtgctcaa ggtggagcgg
     1561 gccggcatcg tgccgactgg agattgagcc gatatgctga gcactcttag gcatctgatc
     1621 gtaggctccc cgctcaccac tcaacagctc agctcggaac gagttaacaa ggtccgggcc
     1681 ctggcggcct tctcgccgga cgcgctctcg tccatcgcct acgccaatca ggaaatcttc
     1741 cttgggttgg tcgtcgccgg gagcgccggg ctggctctca gctggccgat cgggctggcc
     1801 atcaccggcc ttctgatcct tgtggcgttc tcctatttcc agaccgtcca cgcctaccca
     1861 tccggcgggg gctcctacat cgtagcgcgc gaaaacttgg gcacgttgcc cgggttggtg
     1921 gccgcggcgg ccctgctcat cgactacgtc ctcaccgctg cggtgagcct cacggccggg
     1981 gtggcggcga tcgcctcggc gtttcccggg ttgtggcccc accgagtcac attggccttg
     2041 ctcggcttgc tgctcatcac tttgctcaac atgcgggggg tccgcgagac cggcacggta
     2101 atcgccatcc ccgtgtacct gttcctcgct acttacctgc ctatgctggc ctacggactc
     2161 gtgcgggcat tgatcgaggg gccgggatcg tttcatgccg ctgcgctgcc gacgctacaa
     2221 ccgctcactg ccttactgat cctgcacacg ttttcggccg gctgtaccgc cctgaccggc
     2281 atcgaggcca tcagcaacgg agttccggcc ttccggcctc ccgaatccag aaacgcagga
     2341 atcaccctga tcgtgatggc tgtcttgatg gcggtgctct tcgccgggag cattggcctg
     2401 acccaatacc tggcggtcgt tcccggacca gaagagacca tcctctcggc gctggcccgg
     2461 caattgttag gtagcgggct gggctacgtc acaattcaag tgagcactct gctgatcttg
     2521 gtcgtggcgg cgaacaccag ctttgccgga ttcccgcgtc tcgcttcgat cttggcccgc
     2581 gatgggtttc tcccgcgcca gctgtccgcc cttggggacc gactggcctt caacaatgga
     2641 atcctggtcc tgaccgctgc cagcgccgcc cttatcgtca tctttcaagg agacactcat
     2701 gccctgatcc ccctattcgc ggtcggggtg tttctcgctt tcacgctctc ccaagtcggc
     2761 atggtcgtcc attgggctca ggagcggaag ggcagctggg cggtcaaggc cggaatcaat
     2821 ggtgtcggcg gtctgctgac tggaatgact ttggttatcg tcggcgtgag caaattcgtc
     2881 gaaggggcct ggatcagcct gctgttgatt ccgctgctgg tcggcatctt ccttccgatc
     2941 cgggcccact atcgggagct ggcccggcag ctctccctcc agggttcctc cccttcgctt
     3001 cagaagtttg cccctatccg ggctgtggtt cccatctccg gcgtgcatcg aggagtcgtt
     3061 gacgcgatta acttcgcccg cgcgatcacc aagaatgtca cggcggccta tattgagctg
     3121 gagcctggat ccggcgagaa ggtacaacag gagtgggccc gctcgtggcc ggatgtcccg
     3181 ttagtgatca ttccctctcc gtatcgttcg gtcatcggtc cactgctcga ctacctggac
     3241 ctcattgaca ctcgctacaa cgacggccag gcggccgtcg tggtcctgcc cgagttcatt
     3301 ccggcgaagt ggtggcagag cctgctgcac aaccagaccg cctggttgat caaggtcgcc
     3361 ctgctctatc gccggcgccg gaagggtttc cagcgggtga tcatcgatgt tccttaccac
     3421 accgcccgct gagacccgca gaatttcccc gttcgacata aactcccact tcgcgtgcac
     3481 cggccgattg aggcgacttg atgatcacga tttataagaa tggcgtgcag gggctgcagc
     3541 cggcggaggc ggtggagaac ggttgctggg tgcacgcggt agctcccacc gagcaagact
     3601 tggccgtgct ggctgggcac ggcgtcccgc cggagtttct ctcccacttg ctcgatctca
     3661 acgagcgccc acgggccgag cgggaaggcg acgcgatcct gctcgtgctg cattttccct
     3721 atgctcaggg cccgatcgcc gacattccct acatcacggt tccgctgacc atcatcgtca
     3781 ccacggatct ggttgtcacc agcgtcccga tggccaccgg attcctgcag gggttcgcca
     3841 gccgaaatct gcgcggcatg acccccgacc gcccaaccgg cttcgtcctg cagatgttgt
     3901 tgtatctcgc caaagagtac ctggataacc tgaatgtgat caacgcccag gtggatcggc
     3961 tggaagacca gcttcagcga tcgctgcgca atcaggaagt gctgggactg ttgaagttcg
     4021 agaagagcct cacctatttc accaccgctc tccgctcgaa cgaggtggtc ttgggccgac
     4081 tccagcgggg aaacctctcg cagtggcacc cggacgaaat ggaactcttg gaggatgtct
     4141 tgatcgagtt gcggcaggcc atcgagcagg tcgatatctc ccaaagcatc ctggctcaga
     4201 tgatggacgc ctttgcctcg atcgtctcga acaatctcaa ctcagtcttg aaactactga
     4261 cctcggtcac cctgctggtc agcctcccca gcctgatcgc cagcgtttac gggatgaacg
     4321 taatcctgcc gggggcagag tcgccgctgg ccttcgccgg gattctgggt gcctcgatcc
     4381 tggtttcgtt gggcctgctg gtgatcttct ggcgcaaaga ctggctgtag ctgagcggga
     4441 tcagtcccga tccccgtcga gttggtaagg gacatcggtg atgacgaccc cggtctccag
     4501 tggtagggag agacgcagca aggacgccat ttgggtgcgc atcaggttgg accaccagcg
     4561 cggccggact gactgaggga ctacgatggt catcgactcg tgcggctgtc gaagttccat
     4621 catttccacg atgtacttga gcagcggctc caataccatc ccgtgatcgg ccggtagcac
     4681 caccagccgg atgccgtccc cccggcggtc ccactcgttg cggagcgtgt cggcatcggc
     4741 ctgatcgagt gccacgtgca ccgcggttac atcatttgag agcgaggtcg cataacgcaa
     4801 ggccgtcagg gtgccttggt gcaccgcgtt gatcgggagg atgatccggt ggcgggtgat
     4861 ccgaggcggg gccggctcgt ccgccaccgg ggccggggtc ttcacccggt gggcaggggt
     4921 ggttggcggg ttggcttggc gaggctcagc ccgggtggca gagggcttag gcagaatcct
     4981 ccccccattc gccagggcga acgccggcaa tcggcgggcc tgctggccga ccccgaacag
     5041 ctccagaaaa ctggcaatcg cgatcatgag ccggtagcgg tactcctgca tgcggaaggc
     5101 ctgccactcg acgatcgagg ccatcgcgcc ttggacccgg gcctttgtgc gcacaattcg
     5161 gtagatctgg cgtggctcgc ggtagccgaa aggggagagc aagacgacat gggcgacgat
     5221 tgccagaaag ccgaatccaa gcaaggcaat ccagccgcct tcttcccact tgccgatcaa
     5281 ctggccgagg aacacaatcc cggccagaac tgtcacgaac gtggcgcctg cggcgcccca
     5341 gcggcgggcg cgtccagtgt aatgagtgag taagtgtttg cgaactgcca atcccatggc
     5401 tgttatcggc atgaagaccc cgacgccgta gaacggaacg gccaccgtag ttcggccgcg
     5461 tactaggaac atgatcagca ccgccattgc aaacgttatc gtgaccgaac gggtgaatgt
     5521 cccgcgccgg tcgcggtaga tgatggcctc cgggattcct cccatggcca catcgcgcca
     5581 ctcggtggcc tgcgcgtcct gtagcgcggt catcgaggcc gctgccagca tcatcaccgc
     5641 gacgatctgg taggcccaga acaggagaat gccgcccgga atctcaccga aggcgataaa
     5701 ggccaggttg ccgaccaacg tacggccaaa cgtgccgtca aacacgtcga atcgaatggc
     5761 gaagacggtc agaaagaagg tggtcagccc gccatagaat agaaaggaag tctgcacgaa
     5821 gcgtccgatg cccgactttc cgctgtagaa attccagagc cagcttagcc tcgacaggcg
     5881 cttcttaccc catttcacaa tccccgcatc ttcgtcctgc atgaactgga ttccattcga
     5941 caccgcttcc agtccggtca gggcaaccat gcctttcatc gccgcggtca gcatatgcag
     6001 catcgcctgt ccaagcgtga cggctcgcgg cgcggcttca ggcggaaccg gcggaacccc
     6061 tctgaaatgc gccatgatca gcccgatggc catcgcgatg gtcagcagcg cgaaaacagt
     6121 gatcaacgag aacacaatct gcgccgattc gccccggccg cggatggtta ggtaccaagt
     6181 tatggcggcc aacacggccc ctaacccaaa gtgatacagc cagttcttgc cgtacagtcc
     6241 gatgatggaa agcagctgat ccccaccgga cagggccgag accacgatgg tgagtacgta
     6301 gtcctgaatg agcgcgactg ccacgatgag actgagggtt ggccacaagt agcgcatgga
     6361 ggcggtatag gatccgccgc cctcgttgaa cggaccgagc gagaacatgt acagcaggcc
     6421 gaaggcaaag ttggcgaagg cgatgacggc aaccgccaga taggctctgt cttccaaccc
     6481 atagggatgc aaggcgctca tcatttcgcc ggtggcatag taataagaag tgaaatagtc
     6541 caccccgaaa agtgctagcg cggcgagcag cccgatctgg ccggcgccat gaacctgggc
     6601 ggtctccctt cgttcgcggg gcgcgagccg gaaggctagg aagacgatca gcaggagcaa
     6661 aatgagggag agcatctcat gaccttgttg aggattggga agcgattagc taggcttgga
     6721 cgcgccgagt tgaccagcgc tcccacagcc agatcagacg gatctccacg atgatcgaaa
     6781 tcgccatcag tacgtcgtgg aaccggccgg aggccagtgg ctgcaagcgg acctgcagcc
     6841 agaagaaacc caggacaagg ccgaacggga tcatccaatc taagactcgc aacatcaaac
     6901 ccttcataca gctcaaccct agcacctgga aataaatccg gcacaaagcc cgggaccaag
     6961 tgcataaagg acgaataaaa caaaagacgc ccagtgcggg cgtctgaagc taggagaagc
     7021 gccagcggct agtcgtcggc caggaagcgg tagccaactc ccggctctgt cagtacgaac
     7081 tgcggtcgtt ccgggtccgg ctcgagcttc ttgcggagct ggcgtacata gacccggagg
     7141 tactcaacat ggtcggcctc ggtcggcccc cagaccgagg tcagaattcc gcgatgggtg
     7201 agcacccggc cggcgttcgc cgctaggtag gccagcaatc gaaattcggt cgcagtgagt
     7261 ttgacctcct gcccgtcccg ccgaacggta tgtagcgcca agtcgatgct gatcggaccg
     7321 gcagtgacga cagtctcgcc gaggcttccc tgcatccgag cgtcatggcg gagcgccacc
     7381 cggatccgag ccagcagttc ctcaatgctg aagggcttgg tcaggtagtc gtcgccgccc
     7441 ttgtcaagcg cctcgacttt gtccgcgtct gcctctcgga ccgagagcac gatgatcggg
     7501 atctgagtcc actcccgcac tcgctcgatg actgtgaggc catccatgtc cggcagaccc
     7561 aggtcgagga tgatcacgtc gggcggatcc gcggccgccc gggccaggcc ttcctctccc
     7621 cgggtggcgg tggtgacctc gaagcgtttc tcgcccaaga tcgtgcgcag cgcgcgcaga
     7681 atctgaggtt catcatcaat aaccaggacc ttgggacggc tcgtcattcg gcttccggcg
     7741 gcaggttggg aggcgggttc ccatcccagg tcatgggtaa ggtgaagttg aaggcgaatc
     7801 caatgggaat gttttcagcc cagatcctcc cgccatgggc ctccacgatt cctttacaga
     7861 tcgagagccc caatccagtc ccggtcaccc gatcggcggc ggtgatccgg tagaacttgt
     7921 cgaaaattcg ggccaggtcc cgctccggga ccggcggacc ctgattgctc acctggacct
     7981 ggagcgagcc gtccccggtc ggccgagccg agacctgaat ccgagtgtgg ggaggtgcat
     8041 acttcaggca gttgctgacc aagttagtga agacctgttc gatctgcacg tagtccaccg
     8101 ggacgagtgg aagttcctcc ggtatatcga gctccagtcg gtgttgaccg gtcaatggcc
     8161 gcatgcgttc cacaaccccg ccgacaattt cctggagcag gttccattgg cggctcggtc
     8221 gcagcgcgcc ggcctcaatc cgtgacatgt cgagcagatt acccaccagt cgattgaggt
     8281 gatcggactc atcgtcgatc gctccgagca ggtctgcccg ggcggccgag tcccacgcga
     8341 cctcgccgct gcgcaggctg gtggcggcgg ccttgattgt ggccagcgga gtgcgcagct
     8401 cgtgtgacac cgaggacagg accgccgatt tcagccggtc gctctcttcc agcacgcggg
     8461 cccgctgctc ggcctcggcc aaccaggccc gctccagcgc gagcgcgccc tggctggcaa
     8521 aggtcctgag caaccgatct tcggaagttg aaatcggagc cgagcctcgc caaaggcgga
     8581 tctccccaag ctgcccgcgt gcagtctgca ggaccgaaat caggctggct ggcgtggggg
     8641 tggtcatggc ctccgccggc agactgacct ccggggcagg gaagtgaccc ccgccagccg
     8701 ccgccagttg cactcgatct gcctggaagg tgctcttgac ctgttcacat aacgtccgtc
     8761 cgatggccga aacgtcaagc gagccagcca aagccgtgct gagctcgtag agccgggtgg
     8821 cctcgcgctc acgagcattg gccgcggcca gcccggcctg agcccgccct accaattggc
     8881 tgataacgac ggccaccacc aggaacacca gcaaaaccag agtgtcctcg gtccgatgga
     8941 cgaccagcgt gaacctcggc tcgatgaaaa agtagttgaa ggcgaggaag gtccagaccg
     9001 cggtcagcac cccagggccc aggccccagt aggcggcgct cagcccgacc ggcaggaggt
     9061 acagcagtgc aatgagtgga gttcctaggt tggagcgcca cagatagagc agagcggttg
     9121 tcaacccgat ggccaaagtt gcaaagacat agcgtgaggc cactcgagcg gtaagcgaaa
     9181 cgcctggcat attcggacct gcggattcca ttatattcgc cggctgtggc tcgacagccg
     9241 atgcggacgc ggctatcatc ggcaaggcga acgagggagg gagaccatga caaccaatgc
     9301 cgccaaggga cgagtggcgg gcatcagtga cgaggcagtc cgggccaaga ccggcaaaag
     9361 ctggtcggag tggatcgatg tgctcgactc ggcgggagcg gccgacatgg accatccggc
     9421 catcgcaaaa tacctctaca acgacctcgg cgttcccgat tggtggtcgc aaatggtgac
     9481 ggtaggctac gagcaagccc gggggcgcag gcgggttcac gagactgctc gaggctttca
     9541 gatcagccgg tccaggacca tggaagtttc ggctgcccgc gccttcaaag cctggagtga
     9601 ggcagcggtc cgcaaccgct ggctgccagg tcggagtttg aaggtccgca aggcgaccca
     9661 caacgagtcc attcggagta cctggtcgga agacggatca gatgtatcgg tggatttcta
     9721 tgggaaggga cctctcaaag ttcaggtggt cgtacagcac tcggggctat ccgatgcgaa
     9781 gcaggccgaa cggatgaaag agttttggac ggcggccctc gttcggctga agactcaact
     9841 cgaatgacct cagcgaaaga attcgagttc cccttggcgc gactagcaag ctcccaatca
     9901 tagaccggct tggatgtgcc aatggcatat gacaagaagc tcgcagatcg gattcgcaag
     9961 gcgctcagca agcagccaaa acttaccgag cggcagatgt ttggggggat cggcttcatg
    10021 cttcgaggca acatggcttg cggcgtcatc ggcgatgaaa tgatggtgcg catagccaag
    10081 gaggagaccg attccgcaat caaggagcct ggagcccgga tcttcgactt ctcgggtcgg
    10141 ccttcgcccg gctggatcct ggtaaatcag caagtgcttt ccaagcctgc cgagttgagg
    10201 gcctgggtca accgaggcgt tgagtacgcg ctgagcctgc cggccaaatg agccgctgag
    10261 tctgagccgg cgcggatggg ggacgtggac cgggcactca tctcacttgc cggcctctcg
    10321 gtcggggatg cgtttgggga gcagttcttc cggctgccct gggatgcgat ccgggagcgc
    10381 cggctgccgc ctggcccttg gccctggacc gacgacacgg cgatggccct ct
//
LOCUS       DATDPQ010000174         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3468560, whole genome shotgun sequence.
ACCESSION   DATDPQ010000174 DATDPQ010000000
VERSION     DATDPQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3468560"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VI520_04095"
     CDS             <1..774
                     /locus_tag="VI520_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLF37099.1"
                     /translation="LYGMHPICEIQFADFIHPAFNQIVSEAARLCYRSGGEWTVPLVI
                     RAPYGGGIGGGLYHSQSVEAFYAHVPGLKVVIPSNPYDAKGLLKSAVRDPDPVMFFEP
                     KKGYRLIRGEVPEGEEILVPIGPARISRPGTDLSVFAYGMMHQYCWEAAERVAQEGID
                     AEVVDLRTLKPVDKQAVLSSVAKTGKALIVYEDNLTGGYGAEIAAILAEEAFTQLDAP
                     VRRLAGPDIPAVPYSHPLQDWFMPNPDKIAAAIRDLAGY"
     gene            855..>2048
                     /locus_tag="VI520_04100"
     CDS             855..>2048
                     /locus_tag="VI520_04100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLF37100.1"
                     /translation="MAIEVIMPQLGESVVEGTVTKWLKRLHERIQEFEPLVEINTDKV
                     DTEVPSPATGTVLQLLVAEGETVRAGTVLALIGEPGENVPEVQPSGRTDGEFAASKPA
                     VAASAPMRQSEPASPVAAFDRQAEVGFISPVVARIAAERGVDLSKVTGSGQGGRITKK
                     DVLSYLEVKKPAAPWEEPASGELFRPTEEVLGEFKPAVTAPSMKVQPLDPVRARIAEH
                     MVRSRHTSPHVTTVMEADLGRVLAHRASNRPAFERDGVNLTLSPYFVAATVEALKQVP
                     IVNSSWSDQGIVLHPQVNVGVAVSLGDKGLIVPVVKNADQLSLLALARSVNDLSERAR
                     SRKLDPDEVQGGTFTITNHGVSGSLLAMPIINQPQCAILGIGKLQKRVVVIENQGPGG
                     EPGDSL"
BASE COUNT          390 a          621 c          691 g          346 t
ORIGIN      
        1 ctgtatggca tgcacccaat ctgtgagatc caattcgcgg acttcattca tccggcgttc
       61 aaccaaatcg tgagcgaggc ggcccgcctg tgctaccggt cgggcggcga gtggacggtt
      121 ccgctggtga tccgggcccc ctacggaggc ggaatcgggg gcggtctgta tcactcacaa
      181 tcggtggagg ccttctatgc ccacgtccca gggttgaagg tcgtgatccc ttcgaatccg
      241 tatgacgcca aagggttgct caagtcagcg gtccgagatc ccgacccggt gatgttcttc
      301 gaacccaaga agggctaccg cctgatccgt ggcgaagtgc ccgagggcga ggagatcctg
      361 gtcccgatcg gcccggcccg gatatcccgt ccgggcacgg acctcagcgt gttcgcctat
      421 gggatgatgc atcagtactg ctgggaagcc gccgagcgag ttgcccagga agggattgac
      481 gcggaggttg tggatttgcg gaccctgaag ccggtggaca aacaggccgt gttgagctcg
      541 gtggccaaga ccgggaaggc tctgatcgtg tacgaagaca acctgaccgg cggctacgga
      601 gccgaaatcg ccgcgattct ggcggaggag gctttcaccc agctcgacgc gccggtgcgg
      661 cggctggctg ggccggatat tccggcagtt ccgtacagtc atccgctgca agactggttt
      721 atgccgaacc cggacaagat cgcggctgcc atccgcgatc tggccgggta ttgatcgagg
      781 gcaatcacaa atggtcgacc gaccgaggcc cgcggccgca ggccggtcgt gccgaccctg
      841 agaggataga acgtatggcg atcgaagtca tcatgcccca gctcggcgag tcggtcgtcg
      901 agggcacggt caccaaatgg ctgaagcgac tgcacgagcg aatccaggag ttcgagccgt
      961 tggtcgagat caacaccgac aaagtcgaca ccgaagtgcc ctccccggct accggaaccg
     1021 tgctgcagct cttagtggcc gagggtgaga cggtgcgagc cggaaccgtc ctggcgctga
     1081 tcggagagcc gggtgagaac gttccggaag ttcagccctc cgggcgaacc gatggggagt
     1141 ttgccgcttc caagccggcg gtagctgcga gtgcgcccat gcgccagtcg gaaccggcca
     1201 gcccagtggc agccttcgac cggcaggccg aggtggggtt catctctccc gtggtggccc
     1261 ggatcgccgc cgaacgtgga gtggacctga gcaaggtgac cggaagcggt caggggggcc
     1321 ggatcacgaa gaaggacgtt ttgagctact tggaagtgaa gaagccggca gccccatggg
     1381 aggagccggc ctccggcgag ctcttccggc cgaccgagga agttctcggg gaattcaagc
     1441 cggctgtcac ggctccctca atgaaagtgc aaccgctgga tccggtccgg gctcgaatcg
     1501 ccgagcatat ggtccgctcg cggcacacca gcccccacgt cacgaccgtc atggaggccg
     1561 atctcggacg ggtgcttgcc catcgggcgt ccaatcggcc ggccttcgag cgggatggag
     1621 tcaacctcac cctcagtccc tatttcgtgg ccgccacggt agaggcgctg aagcaagtac
     1681 cgatcgtcaa ctcgtcctgg agcgaccagg ggatcgtgct tcacccgcaa gttaacgtcg
     1741 gagtggccgt ctcgctgggt gacaaggggc tgatcgtgcc ggtggtgaaa aacgccgacc
     1801 agctctcgct tctggcgttg gcccgatcgg tcaacgatct gtccgagcgg gcccgcagcc
     1861 gcaagctcga ccccgacgag gtccaaggtg ggacctttac catcaccaac cacggcgtga
     1921 gtggctcgct gctcgccatg ccgatcatca accagccgca gtgtgcgatc ctggggatcg
     1981 gcaagctgca gaaacgggtg gttgtgatag agaaccaggg acccggcggg gagcccggcg
     2041 acagcctg
//
LOCUS       DATDPQ010000175         4651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_340740, whole genome shotgun sequence.
ACCESSION   DATDPQ010000175 DATDPQ010000000
VERSION     DATDPQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340740"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3388
                     /locus_tag="VI520_04105"
     CDS             <1..3388
                     /locus_tag="VI520_04105"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF37101.1"
                     /translation="ARLLGEAKAIAKLNHPHIVAVYDAGEIEKAPFVVMELIAGKSLH
                     EAPPKDFASIAAVAGHICRALEHAHAQGIVHRDLKPENVVLLPDGTAKLMDFGIARSV
                     TSRMTSEGNIIGTVFYLAPELALGQEFDGRADLYALGVMLYELTTGSLPFSHSDPLAV
                     ISQHLHASVVPPRAKRPEVPPLLDELILHLMSKRPEDRPASAGEVLEALQRSDLLDPE
                     ARAEQELRVIDRIARGRFIGREQELNEARELWGKAASGQGQTLLISGEPGIGKTRLMR
                     ELATHVEVTGGRVLVGTCYAEGGAPYAPFAQIVRRTLGKGSQNGNPLPEFVLADLLSL
                     APDLKPYYPGLPPNPPLEPQAEQQRLFENVVAFCGTLSEESPVLLVLDDAHWADSGSL
                     SMLRHLARRTRRNRVLLMATYREVELNESRPFHEVMLDLNRERLATRLKLARLTREET
                     QALLAAIFEENITPDFLSGIYRETEGNPFFIEEVCKTLVEEGKLYFENGSWHRPSMDE
                     LEIPQSVRVAIQSRITTMPEQFQETLRLAAILGREFDYDTLAKASEVSEDALIEALEA
                     AEHNQLIEEVSGRSGVTFSFLHALIPATLEESVHTLRRRKLHRQAAAAIESLRPEDHE
                     ALAYHYGEAGDDQKALTYYTRAGERALAAYANQEAESHFRAASNLAQTDPDKAHLLSQ
                     LGSALANQSRFEDAIQAWHSAIEHYKSIGNLDRMAWCYARSARAVWEAGDFPRGLKLA
                     EQGMQAVEGAPASADVADLLHETARAAHFNGVQDKAASLTQRALEMAEQTNAVRVQAE
                     SLITYGVLDGVPGDEAIAALERAVELARVHNLPETESRARNNLSVTLGIFKGEVQSAR
                     EHLQQAAAVARRLGQAASELFFRSGDVGWAISQGALAYAEAELPKLQELYEQASRPRT
                     SGIVFHRLNGNLARDRGELSEAAELYRENLAQARAGKQLQDIWFTGCLLADVLTEMGQ
                     LAEAEEILLEFIEIGDKIGGQALPRCLISIVYAKRGELSSAQTRFAEAEAEENKNPAA
                     FGAAWLANAKAHLRQAEANWDETWVTFERCASQWERMGMRPDRSRVLRDWAEAHLSRG
                     EPEDGARARELLAEARAEFQAMGALAYVDKIDAQLKELTK"
     gene            3437..>4651
                     /locus_tag="VI520_04110"
     CDS             3437..>4651
                     /locus_tag="VI520_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37102.1"
                     /translation="MNSQAIPKILNDRYRLGEELGRGGMGTVHRAHDSLLQRDVAIKV
                     LRKAGADGGPHAHLLREAQVIAKLNHPNIVQVHDAGVLDGAPFIVMELVEGDSLHRRR
                     PTALLEILSIARQVCAALEHAHAHGIVHRDLKPENVLIGDDGTAKLTDLGLARSGART
                     AAQGEIGGTVFYLAPELALGQASDGRADLYALGVMLYELTTGQLPFTGDNPISVISQH
                     LQATVVPPSTYRPDLPPALEAIILKLVAKSPDDRFASAREAGEALAGIAIQLLSPMER
                     RSNLPIQLSSFIGRERELGQVKRLLASSRLVTLIGAGGCGKTRLAIEAAAAVGPEIPD
                     GVWLVGLGALSDSNLVANAVASALDLGEESDRPMPDQLTDYLRTKNLLLLLDNCEHLI
                     AACAQLAELLLQA"
BASE COUNT          910 a         1491 c         1469 g          781 t
ORIGIN      
        1 ggcccgactc ttgggtgagg cgaaggccat cgccaagctc aatcatcccc acatcgtcgc
       61 ggtctacgac gccggtgaga tcgagaaggc acccttcgtc gtcatggagt tgatcgcagg
      121 caagagcttg cacgaggctc caccgaagga tttcgcgagt atcgccgcgg tcgctggcca
      181 catttgccgg gccctcgaac acgcccacgc ccagggcatc gtgcaccgcg atctcaaacc
      241 tgaaaatgtg gtcctgctgc cggatgggac cgccaaactg atggacttcg gcatcgcccg
      301 ctcggtgacc tctcggatga cgtcggaggg aaatatcatc gggaccgtgt tctatctggc
      361 gccagagctg gcgctaggcc aggagttcga cggccgggcc gacctgtatg cgctcggggt
      421 catgctgtac gagctgacga ccggcagcct gcccttctct cacagtgatc cattggcagt
      481 catctctcaa catttgcacg cctcggtcgt gccgccccgc gccaagcgcc ccgaggttcc
      541 accgctgctc gatgagctca tcctgcacct gatgagcaaa cggccggagg accggccggc
      601 atcggccggc gaggttctcg aggctcttca gcgttccgac ttgctcgatc cggaggccag
      661 ggctgagcag gaactgcggg tgatcgaccg gatcgcccgc ggccgcttca ttggccgcga
      721 gcaagagcta aacgaagccc gcgagctgtg gggcaaggcg gccagtggcc aagggcagac
      781 cctgctgatc agtggcgaac cgggcatcgg caagacccgg ctgatgcgcg agttggcgac
      841 ccatgtcgag gtcaccggag gccgagtcct ggtaggaacc tgctacgccg agggcggcgc
      901 gccctacgcg ccgtttgccc aaatcgtccg ccggacgctc gggaaaggct cacagaacgg
      961 caacccgctg ccggagtttg tgctggccga cttgctcagc ctcgcaccgg acctcaaacc
     1021 ctactacccc gggctgcctc ccaacccacc attggaaccg caggccgagc agcagcggct
     1081 cttcgagaac gtagtggcat tttgcggcac gctgagcgaa gaaagcccgg tgctgctggt
     1141 cctggacgat gcgcactggg ccgacagcgg ttcgctctcg atgctgcgtc acctggctcg
     1201 ccggacccgc cgcaaccggg tgctgctgat ggccacttac cgggaggtcg aactcaacga
     1261 atcccgaccc ttccacgagg tgatgctcga tctgaatcgg gagcggctgg ccacccggtt
     1321 gaaactcgcg cggttgaccc gcgaggagac ccaagcgctg ctggccgcca tctttgagga
     1381 gaacatcacg ccggatttct tgagcggcat ctaccgcgag accgagggca atcccttctt
     1441 catcgaagag gtgtgcaaga ccctggtgga ggaaggcaag ctctacttcg agaacggcag
     1501 ctggcaccgg cccagcatgg atgagctcga gatcccgcag agcgtacgag tggccattca
     1561 gtcccggatc acgaccatgc ctgaacagtt ccaggagact ctccgcctgg ccgcgatcct
     1621 gggtcgagaa ttcgattacg acactctggc caaggccagc gaggtgagcg aagacgcgtt
     1681 gatcgaagcc ctggaggccg ccgaacacaa ccaactcatc gaagaagtga gcggcaggag
     1741 cggcgtgacc ttctccttcc tgcacgcctt gatccccgca actctggaag agagcgtgca
     1801 cactttgcgg cgccgcaagc tgcaccgcca ggccgcggcg gcgatcgaga gcctgcggcc
     1861 agaagaccac gaagcgttgg cctatcacta tggcgaagcc ggcgacgatc aaaaagcgct
     1921 cacgtactac acccgcgccg gcgagcgagc gctcgccgcg tacgccaacc aggaggccga
     1981 gagccatttc cgcgcagcgt cgaatctggc gcagactgac cccgataagg cccatctgct
     2041 ttcgcaactg ggctccgccc tcgcgaacca aagccgcttc gaggatgcca tccaagcctg
     2101 gcactcagcg atcgaacact acaaatcgat tggaaacctg gatcggatgg cttggtgcta
     2161 tgcccgctcc gctcgcgcgg tctgggaggc cggcgatttc ccacgcgggc taaagcttgc
     2221 ggaacagggc atgcaggcgg tcgaaggcgc tcctgccagc gcggacgtgg ccgatctatt
     2281 acacgagact gctcgcgcag ctcacttcaa cggggttcaa gacaaggctg cctcgctcac
     2341 ccagcgcgcc ctggagatgg ccgagcaaac caacgccgtg agggtccaag ccgagtcact
     2401 gattacctat ggcgtgctgg atggcgtgcc cggggatgag gccattgccg ccctcgagcg
     2461 agcggtcgag cttgcgaggg tccacaatct ccccgagacc gaatcccgcg cccggaacaa
     2521 tctttcagtg acgctgggca tattcaaagg ggaggtccaa tcagctcggg agcacctgca
     2581 gcaggcagca gcagttgcac gcaggttagg gcaagctgcc agcgaactct tcttccggag
     2641 tggagatgtc ggttgggcga tctcccaggg cgctctcgcg tacgcagaag cggaattgcc
     2701 caagttacag gagctgtacg agcaggcctc acggcccagg acttcaggca tcgtctttca
     2761 cagactgaat ggcaacttgg ctcgcgaccg cggggagctc agcgaggctg ccgagttgta
     2821 tcgggaaaac ctagcccagg cacgcgcggg caaacagctt caggacatct ggttcaccgg
     2881 ctgtctgctg gccgatgtcc taaccgagat gggtcagctc gccgaggccg aagaaatctt
     2941 gctcgagttt atcgagatcg gcgacaagat cgggggacag gccttgcctc gttgtctgat
     3001 ttcgatcgtt tatgccaagc gcggagagct aagttccgcc cagacccggt ttgcagaggc
     3061 tgaggcggag gagaacaaga acccggctgc ctttggtgct gcctggctgg ccaacgctaa
     3121 ggcccatctc cgccaagccg aagccaattg ggatgaaacg tgggttacgt ttgagcgatg
     3181 cgccagtcag tgggagcgca tgggaatgcg gcccgatcgc tctcgggttc tccgagattg
     3241 ggccgaggcc cacctatccc gcggcgaacc ggaggacggt gcccgagccc gagaactctt
     3301 ggccgaggcc cgggcggaat tccaggccat gggcgccctg gcctatgtcg acaagatcga
     3361 cgcccaattg aaagaactca cgaagtagaa gctccggtct gaagaatccg gacccacccg
     3421 ctccggcgcc cactgagtga actcacaagc gatccccaag atcctgaacg accgctatcg
     3481 cctcggcgaa gagctcggcc ggggcgggat gggaaccgtg caccgggccc acgacagcct
     3541 gctccaacgg gatgtggcta tcaaggtgct gcgaaaggca ggcgcagacg gcggaccgca
     3601 cgcccacttg ctgcgggagg cccaggtcat tgccaagctc aaccacccaa acatcgtgca
     3661 ggtgcacgac gcgggtgtgt tggatggggc accctttatc gtcatggaac tcgtcgaggg
     3721 cgactcgctc caccggcgcc ggccgacagc cctgttggag atcctctcca tcgcccgaca
     3781 ggtttgcgcc gcgctcgagc atgcccacgc ccacggaatc gtccaccgcg atctcaaacc
     3841 ggagaatgtg ttgattgggg atgacggcac tgccaagctg acggacctgg ggctggcccg
     3901 ttccggtgcg cgcaccgcgg cccaaggcga gatcggcggc accgtcttct acctcgcccc
     3961 ggaactggca ctgggtcagg ccagcgacgg ccgggccgac ctgtacgcat tgggcgtgat
     4021 gctctacgaa ctgaccaccg gtcagctgcc cttcaccggt gacaatccga taagcgtcat
     4081 ttcgcagcac cttcaggcga cggtcgtgcc gccgagcacc tatcggcccg atctgccgcc
     4141 tgcgctcgaa gcgatcatcc tcaagctggt ggctaagtca cccgacgacc gtttcgcctc
     4201 agcccgagag gcgggggagg cgctggcggg gattgcaatt caactgcttt cgccgatgga
     4261 acggcggagc aacttgccga tccaattaag cagtttcatc gggcgcgagc gggagttggg
     4321 gcaggtgaaa cgcctgctcg ccagctcccg cctggtcacg ttgatcgggg ccggtggatg
     4381 cggaaaaacc cggctggcca tcgaagccgc ggccgcagtc ggccccgaga ttccggatgg
     4441 cgtctggctg gtggggctgg gagcactttc cgattcaaac ttggttgcca atgccgtcgc
     4501 ctcggcctta gacctgggcg aagagtcaga ccgcccgatg cccgaccagc tcactgacta
     4561 tctgcggacc aagaacctac tgctgctgct cgacaattgt gagcacttga tcgctgcttg
     4621 cgcgcagctg gccgagctgc tattgcaggc c
//
LOCUS       DATDPQ010000176         4793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3471449, whole genome shotgun sequence.
ACCESSION   DATDPQ010000176 DATDPQ010000000
VERSION     DATDPQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471449"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VI520_04115"
     CDS             <1..824
                     /locus_tag="VI520_04115"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HLF37103.1"
                     /translation="TVHRALVERVPLLDEIVLIDSESTDRTRQIAERLGVPVHVHQRL
                     LPHLGARVGKGEALWKSLLVTRGDLIVWIDTDIVNIHPRFIYGVIGPLLLDRRVQFVK
                     GFYRRPLHVGRKVQAGGGGRVTELTARPLLNLFFPELSGVIQPLSGEYGGRRSVLEQV
                     PFFSGYGVEAGLLIDVFERFGLRAIAQVDMLERIHHNKPLEALSRMSFAIIQAVIARV
                     ERRYGGAILEEVNKSMKLIRYQGGEYFLDVDEVIEGERPPMESLPEYRAKFGERG"
     gene            821..1384
                     /locus_tag="VI520_04120"
     CDS             821..1384
                     /locus_tag="VI520_04120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLF37104.1"
                     /translation="MTELLLVRHGETDWNREGRYQGQTDVPLNATGLAQAEALAESQR
                     GRPLDWVYASDMLRARQTAEILGRVTGAPVQFDRRLREIHLGAWEGQLFVQIQETQGD
                     LLAQRDREPLDNRPPGGESVREVMARALPLLEEIQRRHPDGRVAIVSHGAALAVIKIH
                     VLDLPVEAVWDHVPRNGSVEEVSLVAP"
     gene            1381..2673
                     /locus_tag="VI520_04125"
     CDS             1381..2673
                     /locus_tag="VI520_04125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF37105.1"
                     /translation="MRDGPRHVGFISTRLAGTDGVSLEAEKWCRILTGMGLECFYFAG
                     ECDTPPERTVVVPEAHFRHPVVFDLTQRLFAGPTRPPEVTLQVQTLKDHLKDNLELFV
                     RRFEIDLLIIPNALSLPVNVPLGLAVTELIAETRLPSIGHHHDFSWERSRFAVHAAGD
                     YLRASFPPNLPSLRHVVINSFAARQLALRAGVSSILIPNVMDFDSPAPGPDEYSAGLR
                     SALGLHSGEKLLLQPTRVVPRKRIELAFELTRHLAGDCALLISHASGDEGSAYEAHLR
                     DYAELIGARVIFASDIFAQARKTLPDGRRVYSLADAYREARMITYPSTVEGFGNAFIE
                     TIYHRRPIVMSTYEIFRTDIQPKGFRVIGFEDFVSSETVAQVRQVLDDPSLAAEIAEH
                     NYELGRRFYSFPTLERRLSLLIQDTLGTEPGAGLFSSS"
     gene            complement(2786..4075)
                     /locus_tag="VI520_04130"
     CDS             complement(2786..4075)
                     /locus_tag="VI520_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HLF37106.1"
                     /translation="MSPAVVAGLIFGLAIGLILTDRLDRTIIALVGAVAMIAAGTWLG
                     FYSQEQAIQAVDFETLGLLLGMMILISLLKPTGAFEALALAAARASRGRPAVLLVSLG
                     LVTTVLSMLLDNVTTVVLVVPLTILVSEILALSPAPFLIAEALLSDTGGVGTLIGDPP
                     NILIASAAGFTFQDFLTHTLPVVILAWAATLGLLLLLFRRELSVSPTEPEALANLQPR
                     QALRDPRGAWQVAAVLVGAIVLFFLAPTLHLTNSLIALGAAGLALAWVRPKVDETLRG
                     VEWSVLVFFASLFVIVGGLQGAGVLQALGEAVVPIFQGEPVLASVGMIWFVAVLSAVI
                     DNVPVTIAFIPVITYLGSTGVNPLPLWWAMAFGAGFGGNATIIGSAANVVVVQYSRQT
                     REPISSKLWTRRGLPVMALTCLLASLAMAVAHSHFAR"
     gene            complement(4072..4539)
                     /locus_tag="VI520_04135"
     CDS             complement(4072..4539)
                     /locus_tag="VI520_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLF37107.1"
                     /translation="MKVNAVMKRAVISISQSATFGEALAELCDQRVGTLPVLDTEGRL
                     VGVVSLREALKIVLPDIVESMQDLDFVEDFGAVETAPIEEGLHRRPVQELMEPPLSVE
                     QDAGLIGSYTYMRQHQLTDVPVVDPSGHLVGIASWVDVGVGYLRGRLRADASR"
     gene            complement(4529..>4793)
                     /locus_tag="VI520_04140"
     CDS             complement(4529..>4793)
                     /locus_tag="VI520_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37108.1"
                     /translation="QVSERDLRDDLPLFPSLRAMLSIPERNSRLVFSVVPDDFDLDGL
                     IAATERVLGPLTAADSGIFYVVPVTRTVGVRGADFSSGAQDEG"
BASE COUNT          924 a         1541 c         1481 g          847 t
ORIGIN      
        1 ggacagtcca ccgcgcgctg gtcgagcgag tgccgctgtt ggatgaaatc gtcctcatcg
       61 attccgagtc caccgaccgg acccgtcaga tcgccgagcg cctcggtgtg ccagtccatg
      121 tgcatcaacg gctgctgccc catctggggg cgcgggtggg caaaggcgaa gcgctgtgga
      181 agagcctgct ggtcacgcgg ggagacctga tcgtctggat cgacaccgat atcgtcaaca
      241 tccacccgcg gttcatctat ggagtgatcg gcccgttgct cctggatcgc cgggtccagt
      301 tcgtcaaggg cttctaccgc cggccgctgc acgttggccg caaggtccag gcgggcggcg
      361 gagggcgcgt caccgagttg accgcccgcc cgttgctcaa cctcttcttt ccggagctct
      421 ccggtgttat tcagccgctg tccggcgagt acgggggccg acgatcggtg ctcgaacagg
      481 ttcccttttt ctcggggtac ggggtggagg ccgggctctt gatcgacgtc ttcgagcgct
      541 ttggcctgcg agccatcgcc caggtcgaca tgctggagcg cattcatcac aacaaacccc
      601 tggaagccct gagcaggatg tcattcgcca tcatccaagc tgtcatcgcc agggtcgaac
      661 ggcgctacgg cggcgccatt cttgaagagg tcaacaagag catgaagctc atccgctacc
      721 agggcggcga atacttcctc gacgtcgacg aggtcatcga aggcgaacgc cctccgatgg
      781 aaagtctgcc cgaatatcgg gcgaagttcg gagagcgggg atgaccgaac tcctgctcgt
      841 ccgacatggc gagacggact ggaatcgcga gggccgctac caaggccaga cggatgtgcc
      901 cctcaacgcc accggtcttg cgcaggccga ggcgctggcg gaatcgcagc gcggccggcc
      961 tctggactgg gtctacgcca gcgacatgct gcgtgcgcgc cagacagcgg aaatccttgg
     1021 ccgggtcaca ggagctccag tccaattcga ccgtcggctt cgggagatcc acctcggggc
     1081 ctgggaaggc caattgttcg tccagatcca ggagacccaa ggcgacctgc ttgcccaacg
     1141 agaccgcgag ccgctggata accgtcctcc cggaggagag agcgttcggg aggttatggc
     1201 ccgagccctt ccgctgctcg aggagatcca gcgccgccat cctgacggcc gggtcgccat
     1261 cgtctcccac ggtgcggctc tggccgtcat caagatccac gtcctcgacc ttccggtaga
     1321 ggctgtctgg gatcacgtac cgcgcaacgg gtcggttgaa gaggtttcgt tggtggcacc
     1381 atgagggatg gtccgcgtca cgtcgggttc atctccaccc gcctggccgg gacggatggc
     1441 gtctcgcttg aggcggagaa gtggtgtcgt attctcaccg gcatgggcct tgagtgtttc
     1501 tacttcgccg gcgagtgcga cacgcctcca gagcggaccg tggtcgtccc cgaagcgcat
     1561 ttccggcatc cggtcgtctt cgatctgacc cagcgcctgt tcgccggtcc aacccggccg
     1621 ccggaggtca cgctccaggt acagactctg aaggatcatc tgaaggacaa cctggaactt
     1681 ttcgtacgcc gtttcgagat cgacctgttg atcatcccga acgcgctgtc cctgccagtc
     1741 aacgtcccgc tcggattggc agtgaccgag ctcatcgccg agacgcgtct cccgagcatc
     1801 gggcaccatc acgatttttc gtgggaacgc agccggtttg ccgttcacgc cgcgggagac
     1861 tacttgcggg cctcctttcc gcccaacctg ccgtcactgc ggcatgtggt catcaactct
     1921 ttcgccgccc ggcagcttgc cctgcgagcc ggcgtcagtt cgatccttat tcccaacgtc
     1981 atggattttg attcgcctgc gcccgggccg gacgagtaca gcgccggtct gcgctcggcc
     2041 ctcgggctgc actccggcga gaagcttctg ctccagccca cccgagtggt gccacgcaag
     2101 cgaatcgagc ttgccttcga actgacccgg cacctggccg gcgactgcgc cctgctcatt
     2161 tctcacgcct ccggcgacga aggctcggcc tatgaggccc atctgcgcga ctatgccgaa
     2221 cttatcggcg cacgggtgat cttcgcctca gacatcttcg cccaggctcg aaagaccctt
     2281 cctgacgggc gccgggtcta ctccctggcg gacgcctacc gggaggcccg gatgatcacc
     2341 tatccgtcga cggtcgaggg tttcggcaac gctttcatcg agacgatcta tcacaggcgg
     2401 ccaatcgtga tgagcaccta tgagatcttc cgaaccgaca tccagcccaa gggtttccgg
     2461 gtcatcgggt ttgaagactt tgtcagctcc gagaccgtcg cccaggtgcg tcaagttctc
     2521 gacgacccga gcctggcggc cgagatcgcc gaacacaatt acgagctggg gcgacgcttc
     2581 tactcattcc ctacgcttga gcggcgcttg agtctgctca ttcaagacac ccttggcacc
     2641 gaacctggag ctggtctgtt ctcgtcctcc tgacccttgg gcgcgcccag tcaggctgcc
     2701 atcggattcc gaggaagtca accacggtat ttaaggaggc gtcgatgatg accgcgaccg
     2761 ggaggtccgg tacatttcct cgcagttatc tggcgaagtg ggagtgggcc accgccatcg
     2821 cgaggctggc cagcaggcag gtgagcgcca tcaccggcag ccctcgccgg gtccagagct
     2881 tggagctaat tggctcgcgg gtctgccgcg agtactggac cacgaccacg ttagccgccg
     2941 atccgatgat ggttgcgttg cccccgaacc cggcgccgaa ggccatcgcc caccacaagg
     3001 ggagagggtt aactccagtc gagccaaggt aagtaatgac agggatgaac gcgatcgtga
     3061 ccggcacatt gtcgatgact gccgatagca cggccacgaa ccagatcatc ccgaccgagg
     3121 ctaggaccgg ttcgccttgg aaaattggca cgactgcttc gcccagcgcc tgcagcactc
     3181 cggctccctg caggccgccg acgatcacga acaaggaagc gaagaacacc aggaccgacc
     3241 actccacccc gcgtaaggtc tcgtcgactt ttggtcgcac ccaggccagg gctagccctg
     3301 cggcgcccag cgcaatcaga gagtttgtca aatgcaacgt cggcgcaagg aagaagagga
     3361 caatcgctcc gaccaggact gcggctacct gccaggcgcc tcgtggatca cgcaaggcct
     3421 ggcgaggctg aaggttggcc aaggcctccg gctcggttgg ggagacggac agctctctcc
     3481 gaaaaaggag gagcagcagg ccgagggtgg ctgcccaggc cagtatgacc accggaaggg
     3541 tgtgggtgag gaagtcttgg aacgtgaatc cggcggcgga ggcgatcaga atgttcggcg
     3601 gatcgccgat cagggtgccg acccctccag tgtctgaaag gagcgcctcg gcgatcagga
     3661 atggcgccgg agaaagcgcc aggatctccg agacaaggat ggtcagcggg acgaccagga
     3721 ccacggtggt tacattgtcg agcagcattg aaagtaccgt ggtgactaga ccgagggaga
     3781 ctaacagcac ggccggacgc ccgcgggagg cgcgggctgc cgccaaagcg agcgcttcga
     3841 aggccccagt cggtttcaga agcgagataa gaatcatcat cccaagcagc aggcccagcg
     3901 tttcgaagtc caccgcctgg atagcttgtt cttgggagta gaaccccagc caggtcccgg
     3961 cggcaatcat cgccaccgct ccgaccaggg cgataatggt tcgatccaac ctgtcggtta
     4021 ggatcagacc aatggcaagg ccgaaaatca gtccggccac gaccgcggga ctcatcgact
     4081 cgcatcggcc cgcagccgac cgcgcaggta cccgactccg acatcaaccc atgacgcaat
     4141 ccccaccagg tggccggatg ggtccaccac gggaacatcg gtgagttgat gctgtcgcat
     4201 gtaggtgtag ctcccgatca atccggcgtc ctgctcgacc gagagcgggg gctccatcag
     4261 ctcctgtacc ggccgacggt gcaggccctc ctcgatcggg gcagtctcta ccgcgccaaa
     4321 atcctccacg aaatcgaggt cctgcatcga ctcgacaata tccgggagga caatctttaa
     4381 cgcctcccgc aggctcacca cgccgaccag tcgaccctca gtgtcgagga ccggaagagt
     4441 tcccacccgc tggtcgcaca attccgcaag cgcctcccca aaggtcgcag attgcgagat
     4501 cgagatcact gcccgcttca tcacggcgtt aaccttcatc ttgagctcct gaggaaaagt
     4561 cggccccgcg aaccccgacg gtccgagtta cagggaccac gtaaaagatg ccgctgtcag
     4621 ctgcagtaag cggcccgagc actcgttcgg tcgccgcgat gaggccatcg aggtcgaaat
     4681 cgtccgggac gacgctgaaa accaggcggc tgttgcgctc ggggatgctg agcatggcgc
     4741 gaagagatgg aaacagcggc aggtcatccc gcaggtctcg ctcggagact tgc
//
LOCUS       DATDPQ010000177         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2180787, whole genome shotgun sequence.
ACCESSION   DATDPQ010000177 DATDPQ010000000
VERSION     DATDPQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180787"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VI520_04145"
     CDS             <1..549
                     /locus_tag="VI520_04145"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HLF37109.1"
                     /translation="TMEIHHGKHHAAYTNNLNNALESAKEWGEKSIEQILADLDGVPD
                     GIRMAVRNNGGGFANHNLFWTIMGPNGGGEPNGALGKAIGGAFGDFAKFKDELTKAAV
                     GRFGSGWSWLAIDSQGKLKVLATPNQDSLISQGMTPVMGVDVWEHAYYLKYQNRRPDY
                     VTAWWNVVNWAEVGRRFGAATA"
     gene            581..994
                     /locus_tag="VI520_04150"
     CDS             581..994
                     /locus_tag="VI520_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HLF37110.1"
                     /translation="MTHVITSLCLRDGSCVDVCPVECIIPGKPVEQWPWFYIDPDTCI
                     DCGACIPECPYLAIFTEEECPKDYAAKGGEVQSMPVGTAGFDQPWDGKDEHQEPVHVF
                     ATRVIPAGEVVDLTPDIQPNYDYFTKGLGYKALDK"
     gene            1079..2512
                     /locus_tag="VI520_04155"
     CDS             1079..2512
                     /locus_tag="VI520_04155"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HLF37111.1"
                     /translation="MSETRIERDSLGEIEVPGQVLYGGQTMRAVHNFPISGLRPWRAF
                     IWSIATIKRAAAEVNSELGLLDPELAGVIAQAAQEVIDGKWDQEFVVDPFQAGAGTSH
                     NMNANEVIANRATVLLGGGLGEYRANPNDHVNMAQSTNDAIPTAIRLGCLWRLDELLD
                     AVEALGEALRGKAVEFDDIVKSGRTHLQDAVPVRLGQEFGGYATAVARDAERIRRAGD
                     GLRRLGIGGTATGTGLNAHPDFPRLMVGRLAELTGLELRSSTDFFESMQSMADMADFS
                     ASIRTLAITLTRIANDFRLLSSGPATGLDEIRLPAVQPGSSIMPGKVNPVLPEMLNMA
                     MYHVIGCDTTVLLAAQAGQLELNVMMPVLAHNLFEMMQVTIGAVQAFTDRCVRGVLAN
                     REKAQRWLERNAIIVTALNPLIGYAAGAELVKEALAGGHTVFEVARQRVLKGDLRHRD
                     GSRPVTEAELEAALGDLRRLTEGGIVG"
     gene            complement(2534..>2813)
                     /locus_tag="VI520_04160"
     CDS             complement(2534..>2813)
                     /locus_tag="VI520_04160"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37112.1"
                     /translation="GLICWRVLRLDRTRKSIWPYAVTAGWITAQLAWGLHYWPLRPIP
                     AALLLGLAAYVSIGLGRAHLLGRAEPRTVYEFGGVALAVLAAVLLLGD"
BASE COUNT          548 a          818 c          946 g          501 t
ORIGIN      
        1 acgatggaga tccatcacgg caaacatcat gcggcctaca ccaacaatct gaacaacgcc
       61 cttgagtctg caaaggaatg gggggagaaa tcgatcgagc aaatcctggc cgatctggat
      121 ggcgtgcccg acggcattcg gatggcggtc cgcaacaacg gcgggggctt cgcgaatcac
      181 aatcttttct ggaccatcat ggggccgaac ggagggggag aaccaaacgg cgccctcggc
      241 aaggcgattg gcggtgcgtt cggggacttt gccaagttta aggacgagct gactaaggcg
      301 gccgtcgggc gtttcggcag cgggtggagc tggttggcca tcgattcgca gggcaagctc
      361 aaggtgctcg ctacaccgaa tcaggactct ctgatttcgc agggaatgac cccggtcatg
      421 ggtgtggatg tgtgggagca tgcgtactat ttgaagtacc agaatcggcg gccggactat
      481 gtgaccgctt ggtggaacgt ggtcaactgg gccgaagtcg ggagacggtt cggagcggcg
      541 acggcttgaa gtcggtccat caacttttcg gaggagctga atgacacacg tcatcaccag
      601 cctgtgcctg cgagatggct cgtgcgtgga tgtctgtccg gtagagtgca tcatccccgg
      661 aaagccggtc gagcagtggc cgtggttcta catcgatccg gacacctgta tcgactgcgg
      721 ggcctgcatt ccggagtgtc cgtatctggc gattttcacg gaggaagagt gcccgaagga
      781 ctatgccgcc aagggtggag aagttcagag catgccggtc ggtacggcgg gctttgatca
      841 gccctgggat gggaaggacg aacatcaaga gccggttcac gtgttcgcta cccgagtgat
      901 cccggctggc gaggtggtgg acctgacccc cgacatccaa cccaactatg attacttcac
      961 caaggggctc gggtacaagg cccttgacaa gtagccgtct gctgccgtgg aaatctcaag
     1021 gagccttcgg gctccttttt tcttataatt cgaccgggag caggactcga ggaggccagt
     1081 gagcgagacg cggattgaaa gagattcttt gggcgagatc gaagttccgg gtcaggtcct
     1141 gtatggcggg caaacgatgc gggcagtgca caacttcccc atctccggat tgcggccgtg
     1201 gagagcattc atctggtcga tcgccacgat caaacgggcc gcagcggaag tcaactcgga
     1261 gctcggattg ttggaccccg agctggccgg ggtcatcgcc caggccgccc aggaagtaat
     1321 cgacggaaag tgggaccagg aatttgtcgt ggacccgttc caggccgggg ccgggaccag
     1381 ccacaacatg aacgccaacg aagtgatcgc caatcgggcg acggtcctgc tcggcggcgg
     1441 gttgggcgag tatcgggcga accccaatga tcacgtcaat atggcccagt cgaccaacga
     1501 cgcgattccc accgcgattc ggcttggctg cttgtggcgg ctggatgagc tgctcgatgc
     1561 agtggaggcg ctgggcgagg cgctgcgcgg caaagccgtc gagttcgacg atatcgtcaa
     1621 gtccggccgg actcatttgc aggacgcggt gccggttcgg ttgggccagg agttcggcgg
     1681 gtatgcaacc gcagtggcgc gcgacgcaga acggatccgg cgggcggggg atgggctgcg
     1741 ccgcctgggg atcggcggca ccgcaaccgg aaccgggctg aacgcccatc cggatttccc
     1801 gcggctgatg gtcgggcggc ttgccgaact caccggcctc gagctccgca gctcgaccga
     1861 cttcttcgag agcatgcagt ccatggcgga catggccgac ttctcggcct ccatccggac
     1921 gctggcgatc accctgactc gaattgccaa tgactttcgg ctgttgtcgt ccggaccggc
     1981 caccggcctg gacgaaatcc gccttccggc ggttcaaccg ggatccagca ttatgccggg
     2041 caaggtcaat ccagtgttgc ccgagatgct caacatggcg atgtaccacg tgatcggttg
     2101 tgacacgact gtgctgctgg ctgcccaggc cgggcagctg gagctcaacg tcatgatgcc
     2161 ggtgctggcg cataacttgt tcgagatgat gcaggtcacg atcggtgcgg tccaggcatt
     2221 caccgatcgc tgcgttcggg gggtgctcgc caatcgggag aaggcccagc gctggctcga
     2281 acggaacgcg atcatcgtca ccgccctcaa cccgctgatc ggctatgctg ccggagctga
     2341 gctggtaaaa gaggcactgg ccggcggcca tacggtattc gaagttgccc ggcagcgggt
     2401 gctgaaaggt gatctccgcc accgagacgg cagccggccg gtcaccgaag ccgagctcga
     2461 agccgcgctc ggcgatctga ggcggctgac cgaaggtggg attgttggct gaggtttcgg
     2521 aggcccactc ggttcagtcc ccgagcaaga gcaccgcggc cagcaccgcc agcgccacgc
     2581 ccccaaactc gtagaccgtc cggggctcgg ctcgcccgag cagatgagcc cttcccaggc
     2641 cgatcgagac gtatgcggcc aggcccagca gcagcgcggc cggaatgggc cggagcggcc
     2701 agtagtgcag tccccaggcc aactgggccg tgatccatcc ggcagtcacc gcgtagggcc
     2761 agatcgattt ccgggtccgg tcgagccgga gcactctcca gcatatgagc ccc
//
LOCUS       DATDPQ010000178         4281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3472077, whole genome shotgun sequence.
ACCESSION   DATDPQ010000178 DATDPQ010000000
VERSION     DATDPQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3472077"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VI520_04165"
     CDS             complement(<1..929)
                     /locus_tag="VI520_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37113.1"
                     /translation="MTLILAAALLASCNGSSTPATPDPSATPATTSTPVPTSTPLPTL
                     PPTPEATGLELQASALRPGFEGDLAAIANPTRYWIELRVDFDPPNERALIEGLARIRF
                     VNPEGEPITEIVVMLWPNDAQYRAEMSAGPALIDGQLQSPITMLGGLAQRYELRRPLG
                     SGEPVDLSIPFRIATSGPIGGPSPSRFGISEQVLFAPTAYPLLPLRRNGEWVVERAPA
                     GGDTTTSAVAFYQVELTAPDGLEVVTTGVEVSREPLPDGTQALTIVSGPARDFAFAIG
                     PLLSEVREVDGIQVRGWALLGHRPDLTRMVNAAA"
     gene            complement(955..1028)
                     /locus_tag="VI520_04170"
     tRNA            complement(955..1028)
                     /locus_tag="VI520_04170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(992..994),aa:Val,seq:tac)
     gene            1211..2044
                     /locus_tag="VI520_04175"
     CDS             1211..2044
                     /locus_tag="VI520_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLF37114.1"
                     /translation="MPTYEERIRAIRSDLSPSFVVLADFLLDSYIHASFLSATELAHS
                     LDLDPATVVRFAQRLGYDGYPELQREIRDRVREEVLSGRQVEPNSPAEAADLAMTEIA
                     RHLELTRLSFPIETAERLVAALDEAERVIILAEGLAGGPARSLAGWLESAGYTVQLAV
                     GGLSEMARALAGARKRDLAVAIEVVDETPVVSRALAEARRLGLRTAAITASPAAPSTN
                     HAELVISGHASLEPGVGQVIVEALIHALVSVLKRDRPGLFAKVEDKVGELSQRIAGTG
                     S"
     gene            2055..2720
                     /locus_tag="VI520_04180"
     CDS             2055..2720
                     /locus_tag="VI520_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF37115.1"
                     /translation="MILPIVLGVSIVGLLAWELWICEGAHFGRRFVVWLYDLAAGRYD
                     RIKHFDPDWERQVLGEPLSGLLGDLPGASLLDVGAGTGRTFRALGGPGPRQRLVGLEP
                     SRSMLRLGRALVPEEVRWIQGWAVPLPLASESFDAVVCLEVTEFTPHPRSTITELRRV
                     LRPGGWLLISNRIGWQAPLILGRTVGRRRFPGFLSGLGFTEVELVPWQVDYDMAWARK
                     PWS"
     gene            2725..3501
                     /locus_tag="VI520_04185"
     CDS             2725..3501
                     /locus_tag="VI520_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLF37116.1"
                     /translation="MRIVRIQRERGSVPLWGWLTDDRVGLLDGSPFGKYRRQPAELAL
                     AGLTLVAPLQPGKIVCVGRNYAAHAAEHGSEVPELPLLFLKPPSSVIGPGETIRLPPQ
                     SSAVEHEAELAVVIGRRGRWITPEESPAHILGYTIGHDVTARDLQQLDSQWTRAKGFD
                     TFCPIGPWIETELDPSDALITCSVNGQLRQMGSTRDMVFPVHELVAYVSSIMTLEPGD
                     LILTGTPSGVGPLLDGDEVTVEIEGIGKLQNPVRTETVAR"
     gene            3536..4090
                     /gene="dcd"
                     /locus_tag="VI520_04190"
     CDS             3536..4090
                     /gene="dcd"
                     /locus_tag="VI520_04190"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLF37117.1"
                     /translation="MGLKPDHWIVRMAHDQRMIEPFAEAQVRDGVISYGVSSYGYDIR
                     VADEFKIFTNVHSAIVDPKHFDPASMIDFQGQICIIPPNSFALARTIEYFRIPRTVLT
                     VCVGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPARIYANEGIAQVLFFEADE
                     ECRVSYADKSGKYQRQQRIELPKM"
BASE COUNT          749 a         1351 c         1356 g          825 t
ORIGIN      
        1 gcggcggcat tcaccatccg ggttagatcc ggtcggtgtc caagtagcgc ccagccgcgt
       61 acctggatcc cgtcgacttc cctgacttcg gagagcagcg ggccaatggc gaaggcgaag
      121 tcgcgggccg ggccgctcac aatggtcaag gcttgtgtgc cgtccggcag aggctcgcgg
      181 ctcacttcga cccctgtcgt gaccacttcg aggccatccg gagctgtaag ctccacctgg
      241 taaaaggcga ccgctgaggt cgtggtgtcc ccaccggccg gcgcccgttc caccacccac
      301 tcaccgttgc ggcggagcgg cagcagcggg taggcggtcg gcgcaaacag cacctgctcg
      361 gaaattccaa atcggctggg ggacggcccg ccgatcgggc cggaggtcgc gatccggaac
      421 gggatgctga gatcgaccgg ctctccggat ccaagcggcc gacgcagttc gtatcgctgg
      481 gccagtccgc ccaacatggt gatcggcgat tggagttggc cgtcaatcaa tgctgggccg
      541 gcgctcatct cggcccgata ctgtgcatca ttcggccaca gcatcacgac aatctccgtg
      601 attggttcgc cttctggatt cacgaagcga atgcgggcca acccctcgat gagcgctcgt
      661 tcattaggtg gatcgaagtc cacccgcaat tcgatccagt agcgggtggg gttggcgatg
      721 gcggccagat ccccttcgaa ccccgggcgg agggcgctgg cttgcagctc gagccccgtc
      781 gcttccggtg tcggtggcag ggtcggaagc ggagtcgaag ttgggaccgg ggttgaggtc
      841 gtggccggcg tggcactggg atccggcgtg gcgggagtgc tcgacccgtt gcagcttgcc
      901 aggagggctg cggccaggat cagggtcatc cggcgccctc gcggacgcct gagatgggcg
      961 gaacaggact cgaacctgcg accccttctg tgtaagagaa gtgctctaac cagctgagct
     1021 atccgcccga ccgcccatta tacagcccgg ctcgcatgga aacccgcact gcgctcggaa
     1081 aaatcccatt tgcagcaaga tctgcaattt ttcttgcctc tcgaggtcgc ttatgctaga
     1141 gtcctgacac gccggagcct accccggcgg cttccgccat catgtccatc aggcaggccg
     1201 atccgaaaga atgccaacgt acgaagagcg gatccgagcg atccgctcag atttgtcccc
     1261 cagctttgtc gtcctggccg atttcctgct ggactcctac atccacgcgt catttctgtc
     1321 tgccactgaa ctcgctcact ccctcgatct agatcctgcc acggtcgttc gctttgccca
     1381 acggttaggc tacgacggct atccggagct gcagcgcgag attcgcgatc gggtgcggga
     1441 ggaagtgctc agcgggcgac aggtcgagcc gaactcccct gccgaggcgg ccgacctggc
     1501 aatgactgag attgcgcgtc atctggaatt gacccggcta agctttccaa ttgagaccgc
     1561 cgagcgactg gtggccgcct tggatgaggc tgagcgggtg atcattctcg ccgaaggact
     1621 cgccggtggg ccggcccgca gcctggccgg ctggttggag agcgcgggct acaccgttca
     1681 gctcgcggtg ggcggcctga gcgagatggc ccgcgccttg gccggcgccc gcaagcgcga
     1741 tctggcggtt gcgatcgagg tcgtcgacga gacaccggtc gtttcgcgtg cgctggccga
     1801 ggcccgccgc cttggattgc gcaccgcagc catcaccgcc tcgccggcag ccccctccac
     1861 caaccatgcc gaattggtga tctcagggca tgcgagtctg gaaccggggg ttggccaggt
     1921 cattgtggaa gccttgatcc atgcgctggt gagcgtgctc aaacgcgacc gaccgggcct
     1981 cttcgccaag gtcgaagaca aagtcggaga attgagtcag cggatcgcag gcaccggaag
     2041 ctgaccctgc tcgaatgatc ctccccatag tgcttggggt ctcgattgtt gggctgctgg
     2101 cctgggaact ttggatttgc gagggtgcgc attttggccg gcgcttcgtc gtgtggctgt
     2161 acgatctggc ggccggtcgc tatgaccgga tcaagcactt cgacccggac tgggagcgcc
     2221 aggtgctcgg cgagcctttg tccgggctgc tcggggacct ccccggcgcc tccctgctgg
     2281 atgtcggtgc cggcactggg cgaaccttcc gcgctttggg cggtcctggt ccgcggcagc
     2341 gtctggtagg gttggaacct tctcggagca tgttgcgcct cggcagagcg ctagtaccgg
     2401 aagaggttcg ctggatccag ggttgggcgg tcccgctccc tctcgcttcg gagagtttcg
     2461 acgcagtggt ctgcctggag gtgactgagt tcactcccca cccgcggtcg accatcaccg
     2521 aactgcgacg ggtcttgcgt ccgggcggct ggctcctgat cagcaaccga atcggctggc
     2581 aggctccatt gatcctcggt cgtacggtcg gccggcgccg gtttccgggc ttcttatcag
     2641 gactcgggtt cacggaggtc gagttggtgc cctggcaggt cgattatgat atggcctggg
     2701 ctcgcaaacc atggagctga gccgatgcga atcgtccgta tccaacgcga gagggggtct
     2761 gttccgctct ggggatggct tacggacgac cgggtcggac tgctggacgg ctccccattc
     2821 ggaaagtacc gccgccagcc ggcggaactc gccctggcgg gcctgacctt agtggccccg
     2881 ctgcagcctg gcaagatcgt atgcgtcgga cgaaactacg cggcccatgc ggccgagcac
     2941 ggatccgagg tcccggagct gcccctgctt tttctcaaac ctcccagctc ggtgatcggg
     3001 ccaggtgaga ccatccggct accaccccaa tccagcgcgg tcgagcatga agccgaactg
     3061 gccgttgtga tcggccggcg cggccgctgg atcacacccg aggagtcacc ggcccatatc
     3121 ctcggctaca ccatcgggca cgatgtcact gcccgggact tgcaacagct cgacagccag
     3181 tggactcggg ccaaagggtt tgacaccttc tgcccaatcg ggccgtggat cgaaactgag
     3241 ttggacccct cggatgcatt aatcacctgc agcgtgaatg ggcagctccg ccagatgggc
     3301 tctacccggg acatggtctt tccagtgcat gaactggttg cctacgtctc ttccatcatg
     3361 accttggagc caggggattt gatcctgacc ggcaccccat ccggggtggg acccctgctt
     3421 gacggcgacg aagttaccgt cgagatcgaa ggcatcggca agctccagaa tccggttcgg
     3481 accgaaactg tcgctcgttg aacccggttg ttcctacgag ctcaggaggc tcaaaatggg
     3541 cttgaaacca gatcactgga tcgttcggat ggcacacgat cagcgcatga tcgagccctt
     3601 cgccgaagcc caggtccggg acggcgtgat ctcctacggt gtctcctcgt atggctacga
     3661 cattcgagta gcggatgagt tcaagatctt caccaacgtg cactctgcca tcgtcgaccc
     3721 aaagcatttc gatccggcct ctatgatcga ctttcaaggt cagatctgca tcatcccacc
     3781 caactcgttc gccttggccc gcacgatcga gtatttccgg atcccgcgga ccgtgctcac
     3841 cgtgtgcgtc gggaagtcga cctacgcccg ttgcgggatc atcgtcaacg taactccctt
     3901 cgagcccgag tgggaagggt acgtcaccct cgagatttcc aacaccactc cactgccggc
     3961 caggatctat gccaacgaag gcatcgccca ggtgctcttc ttcgaggccg atgaggagtg
     4021 ccgcgtttcc tacgccgata agagcggaaa gtaccagcgg cagcagcgga tcgagctgcc
     4081 gaagatgtag gcggcggcgg cccgatcgcc cacccaccgg ctgaggccgt attcccttca
     4141 gccaggcctg ccggccttga caacccagct atatattcta taatttagaa gatatgtcgc
     4201 ggcccaaacc ccccgaagtc ctcgcgcgga tcgaggagat cctgatgtta gcggtgtggg
     4261 agctggggga cgaggcctac g
//
LOCUS       DATDPQ010000179         5793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_859153, whole genome shotgun sequence.
ACCESSION   DATDPQ010000179 DATDPQ010000000
VERSION     DATDPQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859153"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VI520_04195"
     CDS             <1..715
                     /locus_tag="VI520_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HLF37118.1"
                     /translation="FKQIGEACRLLEVSLVGGHTEISHGIDRPIVVGCMLGEVAKEGL
                     VTAAGAQVGDVLLLVGGIPVEATAILAREKADELAGRFEPQFLERCRNFLFEPGISVV
                     RAARLALAAGPVHSMHDLTEGGLLAGLWELAEASGCGLEVDQEAIPILAEGQVLCEVF
                     GLNPLASIASGALLLTAHRSRQAAIEQALTTQGIVVSPIGQVTARAEPEVRLRSAGGV
                     GPAPKPERDEIARLLQQSA"
     gene            936..1325
                     /locus_tag="VI520_04200"
     CDS             936..1325
                     /locus_tag="VI520_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLF37119.1"
                     /translation="MAALDFRSKEPIYRQIVNQIQDRLGSGELEPGQQLPTVRQLATE
                     LGVNFNTVARAYRLLDEQGVISTQHGRGTYALGRRSAARPRRSQLERLARGFAREAAR
                     LGFRPEQVAETIGYVLEEWDQRGRPVG"
     gene            1383..2225
                     /locus_tag="VI520_04205"
     CDS             1383..2225
                     /locus_tag="VI520_04205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HLF37120.1"
                     /translation="MLNGLLMLAMPLGLGVVLRRRAGGRWGLYGAGALTFIGSQVLHL
                     PFNGLILNPQLEALGFGERGLSSGLVLSAVLLGLSSGVFEEGARFLALRFWQRSARSW
                     RESLMFGAGHGGAEAILVGILALVAFLQAMAYRGSDLSQILPADRVATAEAQLAAYWS
                     APWPEALLGAAERAFSLMVHLSLSAMVMRTLTQRKLSWLGAAVGWHALVNTVAVIALF
                     NLSPAATEAVIGLTAGLSLGILFALREPPAKQTSEPGEPLPRLIPATVKPAAVQLEES
                     KYAD"
     gene            2264..3208
                     /locus_tag="VI520_04210"
     CDS             2264..3208
                     /locus_tag="VI520_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37121.1"
                     /translation="MIRTEGLSKRFGANLAVEDLWLEIAPGELFGFLGPNGAGKTTTI
                     RMLTGLIGPTSGQAWVAGHQLGQDDTELRRQVGILTEAPGLYDTLSAERNLAFFAELY
                     GVTNIATQVERYLRLLGLWNRRSEPTATFSRGMRQKLAIARALLHEPRVLFLDEPTTG
                     LDPEAAHLVRDFIEGLKGEGRTIFICTHNLDEADRLCDRVAVFNTRLLALDTPGALRR
                     QIFGRTVVFHLAQLKPEYRETLAAFPFVKAVETVDSKLVVTLNDPESENPALITALVG
                     AGAQLQFVGELRRSLEDVYLHLLNQANPGRSRPEEASS"
     gene            3205..3996
                     /locus_tag="VI520_04215"
     CDS             3205..3996
                     /locus_tag="VI520_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLF37122.1"
                     /translation="MRRAWTIVRKEWAEIFKNRFVLFTVAFLPMLLTAIPLVILYVTG
                     SVGDLGGLITDDLPPNFGQVCEGLEGAACAQYFLVSQFLLLFMLMPLAIPITIASYSI
                     IGEKTRRTLEPVLATPITTVELLAGKAAAAVIPAVLVTWLAFGVFLLGSAFLAVAPAV
                     FSRLTDPLWLTAVLVVGPLLALAAVNVAVMVSSRSSDPRVAEQLSMLVILPLLALFFG
                     QISGLILLNQKLILWMALVLTVIDAGLLAFSVQLFERETILTRWK"
     gene            3987..4574
                     /locus_tag="VI520_04220"
     CDS             3987..4574
                     /locus_tag="VI520_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37123.1"
                     /translation="MEVSQSIRRQAVWVVFIGAFLALLPSAFAQEDAELRLTVRRTFG
                     YRGGDAIQGRFSLEVEGPAELVQVTFVIDGETMARDLEPPFEHSFHTSEYPLGTHRLL
                     AIGNLASGRELSSAERSFEFVSAEASRDAAGRIVGPLLLSVLGLIALGVLAPALLIRK
                     RGFRLREDNYAGGRPEFEAGPDRIRRLIEESRFES"
     gene            4564..5496
                     /locus_tag="VI520_04225"
     CDS             4564..5496
                     /locus_tag="VI520_04225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF37124.1"
                     /translation="MNPEIPAEADPLSGGQPPICDYEGSDYQDRFWTPGERAYEDGAE
                     AAALRRLLPDGGEFLLEIGAGAGRNTPRYSGFERIALVDYSLSQLQRAQESLGKDERY
                     LYVAGDVYRLPFAPGLFDGATMIRTLHHMSDPQAALVSVAEVLKQNSTFVLEYANKRN
                     LRAVLRWLVRAQVWNPFDRAPIEFVKLNFNFHPQSVRAWLAEAGLVVERELAVSMFRL
                     PLFKRILPIGSLIALDTAVQPIGRWILLSPSVFLRARAGRGFNNHQAGAFWRCPVCRS
                     LQIDENPDGLSCLNCGRDWALKNGIYDLRSRLEG"
     gene            complement(5499..>5793)
                     /locus_tag="VI520_04230"
     CDS             complement(5499..>5793)
                     /locus_tag="VI520_04230"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HLF37125.1"
                     /translation="DLPAVAKQADLAAYDRKVLQDFSLPDGHLKTIPAQRKKLEVVLR
                     HVVLRFSAGKRYSEKQVNLILRRIHADTATLRRELVGAGLLQRVQGEYWRPED"
BASE COUNT         1000 a         1687 c         1950 g         1156 t
ORIGIN      
        1 cttcaagcag atcggagagg cgtgccgctt gctggaggtg tcgctggttg gagggcacac
       61 cgagatcagc cacggaattg atcgaccgat cgtcgtcggc tgcatgctcg gggaagtggc
      121 gaaggaaggc ctggtcacgg cggccggtgc gcaggtcggt gacgtgctgc tgctggtcgg
      181 aggaattcca gttgaggcca ccgcgatcct cgcccgggag aaggccgatg agctggcagg
      241 gcgcttcgag ccacaattcc tggagcgctg ccgaaacttc ctgttcgagc cggggatcag
      301 cgtcgtgcgg gcggctcgcc tggcgttggc tgcgggtccg gtccattcga tgcacgatct
      361 aaccgagggt ggattgctcg ccggcctatg ggagctggcg gaagcttccg ggtgcggatt
      421 ggaagtcgat caggaagcga tcccgatcct ggcggaaggg caggtcttgt gtgaggtatt
      481 tgggctcaat cccctggcct caatcgcctc cggggcgctg ctgttgactg cccatcgaag
      541 caggcaggcg gccatcgagc aggcgctgac tacacagggc atcgtggtca gcccgattgg
      601 gcaagtgact gcccgggccg aacccgaagt gagactgcga tcagccggcg gagtagggcc
      661 tgcaccgaag ccggaacggg acgagatcgc ccggctcttg caacagtcgg cctgacctgg
      721 tacaatacta caattataca ttggtacaat ccaacaattg gacactcgtt gtgtaggagt
      781 gccgatcggg gtggcggggg atcgcagtca gaatcgttcg cacgctctga ggtagctgat
      841 caaggcgggg attcgcggga gtaggtcaca cagaaaggtc gtgatgaggg atcaaggtgg
      901 aagatcgtcg tgagccggta gggataggtt gagagatggc cgcgcttgac ttccgcagca
      961 aggaaccgat ttaccggcag atcgtgaatc agattcaaga tcggcttggc tccggtgaac
     1021 tggagccagg tcagcaactc cccacggtcc gtcagctggc gaccgagctc ggggtgaact
     1081 tcaataccgt cgcccgcgcc taccggctcc tggacgagca gggggtgatc tcgacccagc
     1141 acggccgcgg aacctacgcg ctcggtcgcc ggtccgcggc ccgtccgcgt cgatctcagc
     1201 tcgaacggct tgcccgaggc ttcgcccgag aggcggcccg gctgggcttt cgcccggagc
     1261 aggtggccga gacgattggc tatgttctgg aagagtggga ccagcgcggc cgaccggtgg
     1321 ggtaggcacg tttcacgtgg aacctttggc ttaggagcga ggcgatcgac attctggccc
     1381 gcttgctaaa tgggctgtta atgctggcca tgccgttggg gctgggggtc gtattgaggc
     1441 ggcgggccgg aggccgctgg ggcctgtacg gggccggggc gctgaccttc atcggctctc
     1501 aggtcctgca cttgcccttc aatgggctga ttctcaatcc ccaattggag gcgctgggct
     1561 ttggcgagcg gggactgagc agtggcctgg tgctgtcggc ggtgttgctt gggttgtcct
     1621 cgggcgtatt tgaggaaggg gcgcgtttcc tggcgctgcg cttctggcag cgctcggccc
     1681 gcagttggcg ggaatcgctt atgtttggag ccgggcacgg cggggctgaa gccatcctgg
     1741 tcgggatcct ggccctggtc gcgtttcttc aggcgatggc ctaccgcggt agcgacctga
     1801 gccagatcct accggcggat cgggtggcga ccgccgaagc tcaactggcg gcgtattggt
     1861 ctgcgccctg gcccgaggca ctgcttgggg cggccgaacg ggccttctcg ctgatggtgc
     1921 acctgtccct ttcggcgatg gtcatgcgga cgctcaccca gcgaaagcta agctggctgg
     1981 gagccgcggt cggctggcac gcgctagtca atacggtggc cgtcatcgca ctcttcaatc
     2041 tcagcccggc tgcgaccgaa gctgtaatcg gattgacggc tggcttgagc ttagggatcc
     2101 tgtttgcgct gcgcgagccg cccgccaaac agacctccga gccgggagag ccgcttccta
     2161 ggctcatccc ggccacggtc aagccggccg cggtacagct ggaggagtcg aagtatgcgg
     2221 actgaccggc gaccaacgct gaatggcgga cgagagcagg ccgatgattc ggactgaggg
     2281 tctgtcgaaa cggttcgggg ccaacttggc cgtggaggat ctctggctgg agattgctcc
     2341 gggagagctg ttcggctttc tggggccgaa tggggccggc aagaccacca ccatccgaat
     2401 gctcaccggt ctgatcggcc caaccagcgg ccaagcctgg gtggcgggcc atcaattagg
     2461 ccaggacgat accgagcttc gacgacaggt cgggatcttg actgaggcgc ccggcctgta
     2521 cgacacgctg agcgccgagc ggaacctggc cttctttgcg gagctttacg gggtcacgaa
     2581 catcgcgacg caggtcgagc gctacctgcg gctgctcggc ctgtggaacc ggcgcagcga
     2641 gccgacggcc accttctcgc gcgggatgcg ccagaagctg gcgatcgccc gggcactgct
     2701 ccacgagccg cgggtgctgt ttctggacga gccgaccacc ggcttggacc cagaggcagc
     2761 ccatctggta cgcgacttca ttgaagggct gaagggggag gggcggacga tcttcatctg
     2821 cacccataat ctggacgaag cggatcgctt gtgcgatcgg gtcgcggtgt tcaacacccg
     2881 tctgctggcg ctcgataccc ccggagcctt gcggcggcag atcttcggcc ggacggtggt
     2941 attccatctg gcgcagctca agcctgagta tcgggagacc cttgccgcct tcccctttgt
     3001 gaaggccgtc gagaccgtgg attccaaact cgtggtgacc ctcaacgatc cggagtccga
     3061 aaaccccgct ctcatcacgg ccctcgtcgg ggccggggcc caactgcaat tcgtgggaga
     3121 gctgcgccgg tcgctggagg acgtgtatct ccatctgctg aatcaagcaa atccggggcg
     3181 atcgcggccc gaggaggcaa gctcgtgagg cgcgcctgga ccatcgtccg aaaggagtgg
     3241 gcggagatct tcaagaaccg cttcgtgctt ttcaccgtgg cgttcttacc gatgctcctg
     3301 acggccattc cgttggttat tctctatgta actggctctg tgggcgacct tggcggactg
     3361 atcaccgatg acttaccccc gaattttggc caagtctgtg aaggattgga gggagccgcc
     3421 tgcgcccagt acttcctagt cagtcagttt ctactgttgt tcatgctgat gccgctggca
     3481 attccgatta cgatcgcctc ctacagcatt atcggtgaga agacccggcg aacactcgaa
     3541 ccggtgctgg cgacccccat aaccaccgtg gagctgttgg cgggcaaggc ggcggcggca
     3601 gtgatcccgg ccgtgctcgt cacctggctg gccttcggag tcttcctcct cggatcggcg
     3661 ttcctggccg tcgctccggc agtcttctcc cgactgaccg atccgctttg gctgacggcc
     3721 gtgctggtgg tcggcccgct cctagctctg gccgcagtca atgtggcggt aatggtctcg
     3781 tcgcgctcga gcgacccgcg agttgcggag cagctctcga tgttggtgat cctcccgctg
     3841 ctggcgctct tctttggcca aatctccggc ctgatcctgc tcaaccagaa gctgatcctc
     3901 tggatggcgc ttgtcctcac cgtgatcgat gccggattgc ttgcgttttc cgtccagctg
     3961 ttcgaacggg agacgatcct gactcgatgg aagtaagcca atcgatccgc cgccaggcgg
     4021 tatgggtcgt cttcatcgga gccttcttag ccctccttcc gtcagccttt gcccaggaag
     4081 acgccgagct gcggctcacg gtccgccgga cctttggcta tcgcggaggg gacgcgatcc
     4141 aagggaggtt ctcgcttgag gtagaaggac cagcagagct ggtccaagtc acgttcgtga
     4201 ttgacgggga gacgatggcc cgagacctgg agccgccgtt cgagcacagt tttcatacta
     4261 gtgaataccc cctgggaact catcggctgc tggcgatcgg aaacttggcc agcggcaggg
     4321 agctgagcag cgccgagcgc agttttgagt ttgtctcggc ggaggccagt agggatgcag
     4381 ccgggcggat tgtgggccca ctcctgctca gcgtgcttgg gctgatcgcg ctgggcgtcc
     4441 tggcacctgc gctgttaatc cgcaagcgag gattccgcct tcgggaggat aactacgccg
     4501 gcggacgacc ggagttcgag gcggggccgg accggatcag gcgcctgatc gaggagtcgc
     4561 ggtttgaatc ctgagattcc cgctgaagcg gatccgctga gcggcggcca gcccccgatc
     4621 tgtgattacg aaggatcgga ttaccaggac cgcttctgga ctcctggcga gcgcgcttac
     4681 gaagacgggg cggaggcggc cgcgctgcgg cggttgctgc cggatggtgg agaattccta
     4741 ctggagatag gagcaggagc cggacgcaac actccgcgct acagcggttt tgagcggatc
     4801 gcgctcgttg attactcgct ctcccagctg cagcgggctc aagaaagcct gggcaaggac
     4861 gaacgttatc tgtatgttgc aggcgacgtc tatcggctgc cgtttgcgcc ggggctcttc
     4921 gacggtgcga cgatgatccg caccttgcac cacatgtcgg acccccaggc agccctggtc
     4981 agcgtcgcgg aggtcttgaa acagaactcg actttcgtcc tggaatacgc caacaagcgt
     5041 aatctgaggg cggtactacg ttggctcgtg cgcgctcaag tgtggaatcc gttcgatcgg
     5101 gccccaatcg aattcgtgaa gctcaacttc aactttcatc cacaatcggt ccgagcctgg
     5161 ttagcggaag cgggtctggt cgtggagcgg gagctggcgg tgtccatgtt tcgactgcct
     5221 ttgttcaagc gcatcttacc tatcggatcc ctgatcgccc tggatacggc cgtgcagccg
     5281 atcggaagat ggatcctgct gagcccgagt gttttcctgc gggccagagc cggtcgtggt
     5341 ttcaataatc accaggccgg agcgttttgg agatgtccgg tctgccgttc gctccaaata
     5401 gacgagaatc cggatggact cagctgcctg aactgcggtc gagactgggc tctaaagaat
     5461 gggatctatg acctgcggag ccgactggaa gggtgaggct aatcctcggg gcgccagtat
     5521 tctccctgca cgcgttgaag caagccggcg cccaccaact cgcggcgcag tgtggcagtg
     5581 tcggcatgga tgcggcgcag aataaggttg acttgtttct cactgtaccg cttgcctgca
     5641 ctaaagcgga gaaccacgtg acggagcaca acctcgagct tcttgcgctg ggcggggatc
     5701 gtcttcagat gtccgtcggg tagcgagaaa tcctgcagca ctttacgatc gtatgctgcc
     5761 agatctgcct gcttggcgac ggcgggcagg tcc
//
LOCUS       DATDPQ010000180         6931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1906153, whole genome shotgun sequence.
ACCESSION   DATDPQ010000180 DATDPQ010000000
VERSION     DATDPQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906153"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VI520_04235"
     CDS             complement(<1..195)
                     /locus_tag="VI520_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37126.1"
                     /translation="MPNTIAEGGVGVAVGVMVGVEVGVEVNVLVGVGVAVPVGVGVAV
                     AVGVGVGVGVGVGKAASTEVP"
     gene            215..391
                     /locus_tag="VI520_04240"
     CDS             215..391
                     /locus_tag="VI520_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37127.1"
                     /translation="MRDAPEGAPIGFVAEGELLLVLAGPDLRATGFWWLVLKEDGSQG
                     WLVGEFLATLTPTP"
     gene            complement(459..2096)
                     /locus_tag="VI520_04245"
     CDS             complement(459..2096)
                     /locus_tag="VI520_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLF37128.1"
                     /translation="MSSKPANPWWKQAVFYQIYPLSFQDTTGNGKGDLQGIIDRLDYL
                     RDLGVDALWISPCFPSPNADWGYDVSDYTDIHPELGSLNSLDRLLQSAHQRGLRVLLD
                     FVPNHTSDQHPWFQESRQDRDNPKRDWYVWRDPGANGGMPNNWLTYFSGPAWEWNEAT
                     RQYYLHSFLKQQPDLNWRNPEVRQAMQAVLKFWLDRGVDGFRVDAVLPIIKDALFRDN
                     PTLLEPPVGKDMGPAGRQDRRHSANQPEVHTLLREWRQLLDGYPGDRMMAGEVYTMDS
                     ALAAGYYGQNDELHLVLNFSLLNLPWDAQLIRAYVESFEAALPTGAHATVLLGSHDEP
                     RLATRLGEPQARVAAMMLLTLRGAPVLYYGDEIGMPDTPVPTHLRRDPWPEISGLAKH
                     SRDPARTPMQWDSTPGAGFAPPRSKAGEPGTWLPIHPDYRERNVQVQLQQPRSILNLY
                     RRLLRLRRASSALQAGSYRPVAEAPEDSYVYYRAHGDQTVLVALNFAGKDRVLALNDP
                     RPGRILLSTWLDREEQVTLSQCSLRAHEGIVVQLEPG"
     gene            complement(2112..3005)
                     /locus_tag="VI520_04250"
     CDS             complement(2112..3005)
                     /locus_tag="VI520_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLF37129.1"
                     /translation="MSVGYQTARGASSDRLRRFLSGAPVRIVVLLIALVWTLPSAGLL
                     VSSLRPANDITSSGWWTVFVHPFEASVWTVENYVQVLTTHGLGNALLNSVLVTVPSVV
                     IPITIAAFAAYAFAWLEFPGRKFLFIVVVGLMVVPLQMSLIPLLRLYTQLDLNGTFLG
                     LWLAHTGFGLPLAVFLLFGYISTLPGEIIESARVDGASPLTTFTRLVLPLSVPVVASF
                     AIFQFLWVWNDLLVALVFLGTKEEVAVVTARLAALVGRRGEQWHVLTAGAFVGMAVPL
                     LVFFLLQRYFVRGLTAGSVKG"
     gene            complement(3009..4124)
                     /locus_tag="VI520_04255"
     CDS             complement(3009..4124)
                     /locus_tag="VI520_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLF37130.1"
                     /translation="MATSVPVESPRSPTLNRLTAGPLRVLVSLIVPAITFLVLRWAFI
                     FMRDAEANKLAVGIVALIIGVGGVWALYLVTDNLVSILPEGIRESIRPFVFVGPALVI
                     LTVYLVYPVVRTIYLSFMGPRSEEFVGLANYVFALTSDDMLLALRNNVLWLIFVTSFV
                     VSLGLIIAVLVDRIGRWEPIAKSFIFLPMAISAVGASVIWKFVYSFQPIQRPQIGLLN
                     AVYTALGGDPQGWLVLKPWNNFFLIAILIWVLTGFAMVVLSAAIKGVPSELLEASRID
                     GASEIRIFFGVIIPYIRGTLITITTTVLIMVLKVFDIVFVMTGGQFDTEVIANRMFRE
                     MFTFGNFGRSSALAMVLLVAVIPVMIYNVRSLEASKR"
     gene            complement(4242..5588)
                     /locus_tag="VI520_04260"
     CDS             complement(4242..5588)
                     /locus_tag="VI520_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF37131.1"
                     /translation="MKKLTALLALLLVLAFALGACAQPTAEPVVEEPAVEEPAVEEPA
                     VEEPAVEEMPFAGATVTIFNAAGDEQNSVFAENFVNFEAETGIDVIVEGSPDFETVSR
                     VRSEAGDPYDILNFPQPGLMADFARDGFLVDLSSFIDPSAMQAQYAQSWIDMLTVDGG
                     VYGVWHGVDVKSLVWYPKAEFEAQGFTVPQTWDELLALTEQIAGMGVTPWCIGIESGG
                     ATGWPATDWIEDIMLRTAGAEKYDQWTAGELKFDSPEVRNAFAVMEEIWFNPDYVVGG
                     TTGIQTTFFGDSPLGLFEPRTCYFHRQASFIPAFFPEGTVVGEGVDYFYLPPIDPAMG
                     KPVLVSGNLASLAKDTPAGRAVIEFMMSAESVEAEVRNGNAIGPMNGVSLDWYPTDTQ
                     RGFAEILMNADTFRFDGSDLMPGAVGAGSFWTGVTNWVGGASLDEVLATIDASWPQ"
     gene            5958..>6931
                     /locus_tag="VI520_04265"
     CDS             5958..>6931
                     /locus_tag="VI520_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF37132.1"
                     /translation="MLTQERLALAIRLAWTAGVAIVAGLAGEMTSGLAGVLMVWVLLT
                     GFGQAFVGLLPRRIWPVWTTPLADFAFALSAIWLTGPASSPLWWNLLPGGLLLGMSSG
                     MLAGLAFPSLGSLLVMSLGFVSGDIQSAGLAGLGRAVVTVLLLTPAAAGLGHWLRRRA
                     LASRPNPGPDGGAHQGPTPTMLGLATELNSELRRERIPQLIVDLALGGFDQGIRMAVL
                     LRGEDGYGLAAGHPPMGDRPNMLVAQEGLLAEALATGSPTISQQAVDDPAFERLGLAV
                     GARAWVCLPLMNHGQPEGALLLVHPMAGYFHPGRLELLKAIGAQASSAL"
BASE COUNT         1362 a         2135 c         2185 g         1249 t
ORIGIN      
        1 cgggacctcg gtgctggccg ctttgccgac tcctactccg accccgaccc ccaccccgac
       61 cgcaactgcg accccgactc caaccggtac cgccactccg accccgacca gcacgttgac
      121 ctcgactccg acctctaccc cgaccatcac cccgaccgcc actcctactc caccctcggc
      181 aatcgtgttg ggcaccggcg gacagggagc gatattgaga gacgccccag agggcgcacc
      241 gatcggtttc gtggcagaag gtgagttgtt gctggtatta gccggtccgg atctgcgcgc
      301 caccgggttc tggtggctgg tcttaaagga agacggatcg cagggctggc tggtggggga
      361 atttctagcg accctgacgc ctacgccgtg atggtacaga gcctcccggg atcggctcaa
      421 ccgaggggcg gcccacggag cggtcttcgt ttcggggatc agcccggttc tagctgaacg
      481 acaatcccct cgtgtgcccg taagctgcac tggctgagcg tgacctgctc ttctcggtct
      541 aaccaagttg agagcaagat ccggcccggc cgcggatcgt ttaacgccag cacccggtct
      601 tttccagcga agtttagtgc taccagcaca gtctgatcgc cgtgggcacg gtaatagaca
      661 tagctgtcct ccggcgcctc ggctaccggg cggtaggatc ccgcctgaag cgcggaagag
      721 gcccggcgca gccgcagcag cctccggtag agattgagga tcgatcgcgg ttgctgcagc
      781 tgaacctgca cattgcgctc tcggtagtcc gggtggatgg gcagccaggt accgggctcg
      841 ccggctttcg acctaggcgg ggcgaagccg gctccgggag tcgagtccca ctgcatcggg
      901 gtgcgggctg gatcgcgaga gtgcttggcc agtccggaga tttcaggcca gggatcccgt
      961 cgaaggtggg ttgggaccgg tgtgtccggc atcccgatct cgtcgccata gtaaaggacc
     1021 ggagcacccc gcagggtcag cagcatcatg gctgccaccc tggcctgtgg ttcgcccagg
     1081 cgagtggcca ggcgcggctc gtcgtggctt ccgagcagga ccgtcgcatg ggcccccgtc
     1141 ggaagcgcgg cctcaaaaga ctcaacgtag gcacggatca gttgggcgtc ccagggtaag
     1201 ttgagcaggc tgaagttgag taccaagtgc agttcatcat tctggccgta gtacccggct
     1261 gcgagggcgg agtccatcgt atacacctca cccgccatca ttcgatcacc ggggtagcca
     1321 tcgagcagct ggcgccattc gcgcagcaag gtgtgcactt ccggctgatt cgcgctatgc
     1381 cggcgatctt gccggccggc ggggcccata tccttgccca ccggaggctc cagcagagtg
     1441 gggttgtccc ggaaaagggc gtctttgatg atcggcagca ccgcgtcgac ccggaagccg
     1501 tccacgcccc gatccagcca aaacttgaga accgcctgca tcgcctggcg cacttccgga
     1561 ttgcgccaat tcaggtccgg ctgttgcttg agaaagctgt gcaggtagta ctgccgggtc
     1621 gcctcattcc actcccaggc cggcccggag aagtaggtga gccaattgtt aggcattccg
     1681 ccgttcgctc ccgggtcccg ccagacatac caatcgcgct tcggattgtc gcggtcctga
     1741 cgggactcct gaaaccacgg atgttggtcg gaggtgtggt tgggaacaaa atccagcagc
     1801 acccgcagac ctcgctgatg ggcggattgc agcagccggt ccagcgagtt tagcgagccc
     1861 agctccgggt ggatgtcggt gtaatcgctg acgtcgtagc cccaatcggc attcggggag
     1921 gggaaacacg ggctgatcca caaggcatca acccccagat cccgcaagta atcgagccgg
     1981 tcaatgatcc cttgcaggtc ccctttgccg tttccggtgg tgtcctgaaa gctgagcggg
     2041 tagatctggt agaagaccgc ctgcttccac caggggtttg ccggcttgga ggacatgcgg
     2101 gaggagtggg gttagccctt gactgagccg gcggtcaggc cgcgcacgaa gtagcgctgc
     2161 agcaagaaga acaccagcag tgggacggcc atgcccacga aagcgccggc ggtgagcacg
     2221 tgccattgct ctccgcgccg gccgaccagc gccgccagcc gagccgtaac cacggcaacc
     2281 tcttccttgg ttcccagaaa gaccagcgcc accagcagat cgttccacac ccacaggaac
     2341 tggaagatgg caaacgaggc aaccaccggc accgaaagcg gaagcacgag gcgggtgaag
     2401 gttgtaagtg gagaggcacc gtctacccga gccgattcga tgatttcgcc gggcagagtc
     2461 gagatgtagc cgaataagag gaataccgcc aacggcaatc cgaacccggt gtgggccagc
     2521 cacaggccga ggaaggttcc gttgagatcc agttgggtat acagccggag cagcgggatc
     2581 aaggacatct gcagcggcac caccatgagc cccaccacta cgatgaagag gaatttccgg
     2641 ccggggaatt ccaaccaggc gaaggcataa gccgcgaagg ccgcgatggt aataggaatg
     2701 accactgagg ggacggtgac cagcacgctg tttagaaggg cgttgcccaa cccatgggta
     2761 gtgagcacct gcacgtagtt ctcgacggtc cagaccgagg cttcgaacgg atgaacgaac
     2821 accgtccacc atcccgaact ggtgatgtcg ttggccggcc tcagcgaact gaccaacaag
     2881 ccggccgaag gcagagtcca gaccagcgcg atcagcaaga cgacaatccg aaccggtgcg
     2941 ccggagagga acctgcgcag ccggtcgctg gatgcgccgc gggcggtctg atagccgacg
     3001 ctcatagcct atctcttgga tgcctcaagg ctgcggacgt tatagatcat gactggaatg
     3061 acggcgacca ataagaccat ggccagcgcg ctggagcgcc cgaagttgcc gaaggtgaac
     3121 atctcgcgga acatgcggtt ggcgatcact tcggtgtcga attggccgcc ggtcatcacg
     3181 aaaacaatat cgaacacctt gagcaccatg ataaggacgg tggtggtgat cgtgattagc
     3241 gttccccgga tgtaggggat gatcactccg aagaagatgc ggatctcgct ggctccatcg
     3301 atgcgggagg cttccagcag ctcggacggc acccccttga ttgcggccga cagcaccacc
     3361 atggcgaatc cagtgaggac ccaaatcaag atagcgatca ggaagaagtt gttccagggt
     3421 ttgagaacca gccacccctg cggatctccc cccagcgcgg tgtagacggc attgagcagg
     3481 ccaatctgag gccgctggat aggctggaag ctgtatacga acttccagat gacgctggcg
     3541 cccaccgccg agatggccat gggcaagaag atgaaggact tggcgatcgg ctcccaccgg
     3601 ccgatccggt ccaccagcac cgcgatgatg agcccgagcg acaccacaaa gctggttacg
     3661 aaaatcagcc atagcacatt gttgcgcagc gcaagcagca tgtcgtcgct ggtcagggcg
     3721 aaaacgtaat tcgcaaggcc gacgaattcc tccgaccgag gccccatgaa actcaggtag
     3781 atggtgcgga caactggata gaccaggtac accgtaagaa tcaccaacgc cggaccgaca
     3841 aaaacgaacg gtcgaatgct ctcccggatc ccctccggca agatcgagac caggttgtcg
     3901 gtgaccagat acagcgccca gaccccgccc acgccaataa tgagcgctac gatgccgacc
     3961 gcgagtttgt tggcctcggc gtcgcgcatg aagatgaagg cccagcgcag cacaaggaag
     4021 gtgatcgccg gcacgatcag ggataccagg actcgcagcg ggccggcagt cagccgattc
     4081 agcgtcggcg agcggggaga ctcaacggga acgctggttg ccatcggaaa aggcctcctc
     4141 gacatgagaa ggagaaaaag cagggcgacc gtacgccggc cgccctgctt ctggttaggc
     4201 tattggggcg agatcccagt gttcgacccc atagcgagca cttactgcgg ccaactggcg
     4261 tctatagttg ccagcacctc atccagactt gcgccgccaa cccagttggt gacgccggtc
     4321 cagaagctgc cagcgccgac tgcgcctggc atgaggtccg acccgtcaaa gcggaaggtg
     4381 tccgcgttca tcaagatctc cgcgaatccc cgctgcgtgt cggtggggta ccagtcgagc
     4441 gagacgccgt tcatcggccc gattgcgttg ccgttccgga cttcggcctc gaccgactcg
     4501 gcgctcatca tgaactcgat caccgcccgc ccggccggcg tgtcctttgc caggctcgcg
     4561 aggttcccgg agactagcac cggcttgccc atcgcggggt caatcggcgg caggtagaag
     4621 tagtcaacac cctcacccac caccgttccc tccgggaaga aagcagggat gaagctggct
     4681 tgccggtgga agtagcaggt gcgcggctcg aacaggccga gcggcgaatc gccaaagaag
     4741 gtggtctgga taccggtcgt tccacccacc acatagtccg ggttgaacca gatctcctcc
     4801 atcactgcaa acgcgttccg cacttcaggc gagtcgaact tcagctcacc cgccgtccac
     4861 tggtcatact tctccgctcc cgcggtgcga agcatgatgt cttcgatcca gtccgtggct
     4921 ggccagccgg tcgcgccacc gctctcgatg ccgatgcacc agggggtaac gcccatcccg
     4981 gcgatctgct cggttagcgc cagcagttca tcccaggtct ggggaacggt aaatccctga
     5041 gcttcgaact cggccttggg ataccagacc aggctcttga cgtcgacgcc gtgccagacg
     5101 ccgtagacgc ccccatcgac cgtcagcata tcgatccagg actgggcata ctgcgcctgc
     5161 atggcgctcg ggtcgatgaa gctggacagg tctaccagga agccatcccg ggcgaagtca
     5221 gccatcagac ccggctgcgg gaagttcagg atgtcgtaag ggtcgcccgc ttcggatcga
     5281 actcgggaaa ccgtctcaaa gtccggtgag ccctcgacga tcacgtcgat gccggtttcg
     5341 gcctcgaagt tcacgaagtt ctccgcgaat acgctattct gttcatctcc tgcggcattg
     5401 aagattgtga ctgttgcgcc cgcaaatggc atctcctcta cagccggctc ttcaaccgca
     5461 ggttcctcga cggcgggttc ttcgacggca ggctcttcca ccactggctc ggcagttggc
     5521 tgggcgcagg cgcccagggc gaaggcgagt accagcagca gtgcaagcag tgcagttagc
     5581 tttttcatct ctcctctatc ctccatgggg ttatctagta gctttctctt tcgggaccga
     5641 gtggaattga tcggcttgag gctcctcctc tcttgttcct gcggttcttc gactagccat
     5701 atacaaaatg gctgtgcgac tctccggtgt gagaaagacg ggacccggtc ggatgaacta
     5761 gcctccgacc ggcgtcaggc gaagggcaga acccaaccat ggtaatggaa cgcaatgcgc
     5821 gttgtcaagg cggctggaat caaccggccg tccggcttga gcgcgcgctg tcttcgatca
     5881 cgctagcggg tcggtgtctg cctccagatg gcggaccatc cactaccaca tctcgatctc
     5941 cagtagaatc gccccgcatg ctcactcaag agcggctcgc actggccatc cggctggcct
     6001 ggacggccgg cgtggcgatc gtggccgggc tggcgggtga aatgacctcg gggctggccg
     6061 gagtgctgat ggtctgggtg ttgctgaccg gatttggtca ggcattcgtt ggtctgctgc
     6121 cgcggcggat ctggccagtc tggactaccc cgctggctga cttcgctttt gccctctccg
     6181 ccatctggct cactggtccg gcctccagtc cgctctggtg gaatttgttg ccggggggat
     6241 tgctgctcgg gatgagctct gggatgctag ccggattggc cttcccgagc ctagggtcgt
     6301 tgctggtaat gagcctcggc tttgtgagcg gggatattca gtcggcgggc ttggccggct
     6361 tggggcgggc agtggtaacc gtactgcttc tcaccccggc cgccgcgggt ctggggcact
     6421 ggctccgccg gagggcgctc gcaagcaggc ccaatccggg gccggacggc ggagcgcacc
     6481 aaggtccgac cccgacgatg ttgggtctgg caaccgagct gaattccgag ctgcggcgcg
     6541 aacggatccc acagctgatt gtcgaccttg cgctgggagg gttcgaccag ggcattagaa
     6601 tggcagtcct gctgcggggc gaggacggct atggtctcgc cgccggtcat ccgccgatgg
     6661 gggatcgccc aaatatgcta gtggctcagg aggggttgct ggctgaggca ctggcgactg
     6721 gcagccccac gatttcacag caagccgttg acgatccagc tttcgagcgg ttgggattgg
     6781 cggtgggggc gcgagcttgg gtgtgcctgc cgctaatgaa tcatggtcaa cctgaaggcg
     6841 cactcttgct ggtccacccg atggcgggct actttcatcc cgggcggctg gagctcttga
     6901 aggccatcgg cgctcaagcc agcagcgcgc t
//
LOCUS       DATDPQ010000181         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2184735, whole genome shotgun sequence.
ACCESSION   DATDPQ010000181 DATDPQ010000000
VERSION     DATDPQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184735"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VI520_04270"
     CDS             <1..475
                     /locus_tag="VI520_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HLF37133.1"
                     /translation="LITVDPDPTRRAASRDFGAAQSLSPSELQGLREQADLSYELSGD
                     PQALDQAISVTGFEGRVVLGSWYGTKPATLNLGGRFHRSRIRLLSSQVSTVASPLTGR
                     WDKRRRLEFAQQMVQAIKPTRLITHRIPIERAAEAYSLLDTHREHALQVVLTYDS"
     gene            518..898
                     /locus_tag="VI520_04275"
     CDS             518..898
                     /locus_tag="VI520_04275"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLF37134.1"
                     /translation="MFSLALRRSFSASHFLIGGDWGLENLPHSHDYRLEVELEGDRLD
                     RHGFLVDLVELARMVDATLAQYRGQSLNQLAAFRGLNPSLEQFCRILGSSFDQSLQQP
                     GIRSLTVRLWEDESAWASFRTTKT"
     gene            868..1980
                     /locus_tag="VI520_04280"
     CDS             868..1980
                     /locus_tag="VI520_04280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF37135.1"
                     /translation="MGLLPHHQDMNLGLVIYGSLEQRSGGYLYDRRLVEALRQSGDQV
                     ELISLPARSYARNLLDNFDPQLLRRLQTGQYDLLLEDELNHPSLFWLNRRLPAAGAPI
                     VAIVHLLRSGEPRARGLNWIYRRIERAYLRTISGAVFVSRATRAAVEVLVGAEIPGVV
                     AYPGRDLLNGRMDRQEIAARAGQAGPLRLVFLGNLIPRKGLHTVLDALQRLPSDAAQL
                     KVIGSPDLHPVYASRIRRRIGRMNGRVQLLGSLPHDAIKDHLMEAQVLVMPSAHEGFA
                     IAYLEGMGCGLPAVAGSAGGAAELVRDGENGFLVPPNDPVVLSERLARLAGDRALLSE
                     LGQQALASFEAHPTWEQIAGRVRSFLAEVVDHRPKN"
BASE COUNT          354 a          652 c          671 g          366 t
ORIGIN      
        1 cctgatcacg gtcgaccccg acccaacgcg ccgggccgct tcgcgcgact tcggggctgc
       61 gcaatcgctt tccccctccg aactgcaagg gctgagggag caggccgatc tcagctacga
      121 gctgagcggc gacccgcagg cattggatca ggcgatctcg gtcacgggct tcgagggccg
      181 ggtcgtattg ggctcctggt atgggaccaa accggccacg ctgaacttgg gcggccgctt
      241 tcatcgcagc cggattcggc tgttgagcag ccaggtcagc acggttgcct cgccgctgac
      301 cgggcgttgg gacaaacgcc gccggctcga gttcgcacaa cagatggtgc aagccatcaa
      361 gccgacccgt ctgatcaccc acaggattcc aatcgaacgg gccgccgagg cttattcctt
      421 gctggacacc caccgtgaac acgcgctgca agtcgtgctc acatacgatt catgagctgg
      481 aaatctgagt accaggtgaa ggcaggccgc taggcccatg ttctcgctcg cgcttcgccg
      541 cagcttttcc gccagccatt tcctgatcgg cggagattgg gggctcgaaa acttgcccca
      601 ttcccatgac tatcggctgg aggtcgagct ggaaggcgac cggctcgacc ggcacggatt
      661 cctggttgat ctggttgagt tggcgcgaat ggtggatgcc accctggcgc agtaccgcgg
      721 ccagagcctg aaccagttgg ccgcgtttcg tggcctgaat cccagcctgg aacaattctg
      781 tcggatcctg ggcagttcct tcgatcagag cttgcagcaa ccggggatcc gcagcctcac
      841 cgtccggctt tgggaggacg agagcgcgtg ggcctccttc cgcaccacca agacatgaac
      901 ctcggactgg tgatctacgg ctcgctcgag cagcgatccg gaggctacct gtacgaccga
      961 aggctggtcg aggccctgcg gcagagtgga gatcaggtcg aactgatctc cctgccggcc
     1021 cgatcctatg cccggaattt gctcgacaac ttcgacccac agctgcttcg ccgactgcag
     1081 accgggcagt acgacctgct gctggaggac gagctcaatc acccttcctt gttctggttg
     1141 aaccgccggc tgcctgcggc cggagcgccg atcgtcgcga tcgtccacct tctgcgcagc
     1201 ggcgaaccgc gggcccgcgg cctgaattgg atctaccgcc ggatcgagcg ggcttactta
     1261 cgcaccatat ccggagcagt gttcgtcagc cgggccacca gggcagccgt cgaggtattg
     1321 gtcggagcgg agatcccggg agtggtcgcc tatccggggc gggatctcct gaacgggcga
     1381 atggatcgcc aggagattgc ggcgcgagct ggccaggcgg gaccgctgcg gctggtgttc
     1441 ctgggcaacc tgattcccag gaaggggctg cacaccgtcc tcgatgcgct gcagcggctg
     1501 ccctcggacg ccgcacagct caaggtgatc ggttcgccag atcttcaccc ggtgtacgcg
     1561 agccgcatcc gccggcggat cgggcgaatg aacggccggg tgcagttgct tggctcgctg
     1621 ccgcacgacg cgatcaagga tcacttgatg gaggcgcagg tgctggtgat gccctcggcc
     1681 cacgaaggat tcgcaatcgc ctacctggag ggaatgggct gtgggctccc ggccgtcgcc
     1741 gggtcggccg gcggcgcggc cgagctggtg cgggatggtg agaacggatt cctcgttcca
     1801 cctaacgacc cggtcgtgct gtcggagcgg ctagcgcgtt tggctggcga ccgggcccta
     1861 ttgtccgagc tgggccagca agccctggcc agcttcgaag cgcatccgac ctgggagcag
     1921 atcgcgggcc gggtccggtc gtttttggcg gaggtcgtcg accaccggcc taaaaattga
     1981 gccacaccga accattacac ctcaacccga aggagggcac cgatgagtcg atcgaacctg
     2041 cgg
//
LOCUS       DATDPQ010000182         3568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_344761, whole genome shotgun sequence.
ACCESSION   DATDPQ010000182 DATDPQ010000000
VERSION     DATDPQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344761"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="dnaX"
                     /locus_tag="VI520_04285"
     CDS             complement(<1..1060)
                     /gene="dnaX"
                     /locus_tag="VI520_04285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLF37136.1"
                     /translation="MTQVLYREWRPQTWDEVVGQDHVVQTLRNAVRGERLAHAYLFAG
                     PRGTGKTTTARLLAKAANCLAEAPGERPCNRCARCVAVNAGRFLDLIEIDAASNTSVE
                     DVRELRERINFSPNEGRFKVYIVDEVHMLSTAAFNALLKTLEEPPAHAIFILATTEVH
                     KIPATVLSRCQRHEFRRIPVALISDYLDRQIAKQGFRLDRESVDLIARQSTGSLRDAV
                     SLLDQLVSSGEQVTLSLAQQVLGTASTEVVWDLLETLVDGKIGKGLTVLNRALDAGVD
                     PRQLARQVVTSLRAVLLIQMGELPAEEAPAGDHPRLQAVADRIQFDPLLNALRAFSQA
                     TRSQPGNWQPGLALELAYL"
     gene            1190..1369
                     /gene="tatA"
                     /locus_tag="VI520_04290"
     CDS             1190..1369
                     /gene="tatA"
                     /locus_tag="VI520_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLF37137.1"
                     /translation="MPNFGPTELIIILLIALLLFGVGRLGRLGGELGRGIREFRKGIK
                     EDETPEAGSADEKTD"
     gene            complement(1403..2140)
                     /locus_tag="VI520_04295"
     CDS             complement(1403..2140)
                     /locus_tag="VI520_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLF37138.1"
                     /translation="MGGVRRATWKQFLPFLALNVLVSTLTILLVLAVWGRGPKLPSLP
                     PTPTFDLTARLASAIPTATATLPPSPTPVTYTVGPGDSLGAIALELNISMESLMAANG
                     LANPDALAVGQVLVIPQVEGSATAVPEPGDRSASPTAEGAPQVVIRGAYERGDLQTEF
                     VYLENLGGVATMLGWTLDDGEGSVFSFPSFTLYNGGGVNVYTRAGTNSVINLYWGFDQ
                     PLWTPGKQITLRDGQGASHSQFLVPSG"
     gene            complement(2463..3398)
                     /locus_tag="VI520_04300"
     CDS             complement(2463..3398)
                     /locus_tag="VI520_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLF37139.1"
                     /translation="MTENSNDPKPGIWQRVLTRLRGLTPPKAVEPGPDLADTAPLTPE
                     SLEQIAAVRMRESLSAGSAQSVGLERESNEDTLFMMYGNSGGEEVVPDFGLFCVADGA
                     GGLGHGELASSIAVKAVVRSLMEHAVLAGLAQEAESRNVQLDELARQAFARANEAVRE
                     KANGGVTTLTVAILMNHQMTIGHVGDSRAYLVENGVLKLLTRDHSYSQRMVEIGQITP
                     EEALDHPKRNQLWNAVGQGSNMVVEVNSYPVPRGGYLLLCSDGLWGEISMDEVRSVLD
                     LHAEPNEACEELIRAANAAGGPDNISALLVAFSSI"
     gene            complement(3496..3568)
                     /locus_tag="VI520_04305"
     tRNA            complement(3496..3568)
                     /locus_tag="VI520_04305"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3533..3535),aa:Ile,seq:cat)
BASE COUNT          678 a         1147 c         1068 g          675 t
ORIGIN      
        1 caagatacgc cagctccaac gccagccccg gttgccaatt gccgggctgg ctgcgggtcg
       61 cctggctgaa agcccgtaaa gcgttcaaga gcggatcgaa ctggatccgg tccgccacgg
      121 cctgcaggcg cgggtgatcg ccggccggag cttcctccgc ggggagctca cccatctgga
      181 tcagcaggac tgcccgcagc gaggtcacaa cttgcctggc cagctgacgc ggatccactc
      241 ccgcgtccag ggcccgattg agcacggtga gccccttccc tattttcccg tccaccaggg
      301 tctccagcag gtcccacacc acctcggtcg aggcggtccc caacacttgc tgggcgagcg
      361 agagggtgac ctgctctcca gatgaaacca gctgatccag cagcgagacc gcgtctcgca
      421 gacttccggt ggattgacgg gcgatgaggt ccaccgattc gcgatccagc cgaaaaccct
      481 gcttcgcgat ctggcgatcc aagtagtcgg agatcagcgc cactgggatc cggcgaaact
      541 cgtgccgctg gcaacgcgag agcaccgtgg ccgggatctt gtgtacttcg gtcgtagcca
      601 agatgaagat tgcgtgggca ggaggctctt caagggtctt cagcagggcg ttgaacgccg
      661 cggtggagag catgtggacc tcgtccacga tgtagacctt gaatcgccct tcattcggtg
      721 aaaagttgat ccgctcacgc agctctcgca catcctctac gctcgtgttg gaggccgcat
      781 caatctcaat caaatcgagg aaccggccgg cattcactgc cacgcagcga gcacagcgat
      841 tgcacggccg ctcgccaggc gcctcggcca ggcaattggc cgccttggcc agcagccggg
      901 cagtggttgt cttcccggtg ccgcgcgggc cggcgaacag ataggcgtgg gccagtcgtt
      961 caccccggac cgcgttccgc aaggtttgca cgacgtggtc ttggccgacg acctcatccc
     1021 aggtctgcgg ccgccactct cggtagagaa cttgggtcat tgggtgattc ggtccgagtg
     1081 caacagaaac tcgtggggga gccagtgtat cagacgctcg aagccggtgc aaggatcgac
     1141 gtcagattcc gacgttataa tgggtggtgt tcttcagcgg gagccaacca tgccaaattt
     1201 cggtcccacc gaactcatca tcatcttgct gatcgctttg ttgttattcg gagtcggacg
     1261 gctgggccgg ctcggcggag agctgggccg ggggattcga gagttccgca agggcatcaa
     1321 ggaagatgaa actccggagg ctggctcggc agacgagaag accgactgag cccgccagat
     1381 ccggggggag ctcctctgcg agctacccgc tcggcaccag gaactgggaa tgcgaagcgc
     1441 cctgtccatc gcgcaaggtt atctgcttgc ccggcgtcca gagcggctgg tcgaaccccc
     1501 agtagagatt gatgacggag ttggtccctg ctcgggtata tacgttcaca ccgcctccgt
     1561 tatagagggt gaagctgggg aacgagaaca cacttccctc cccgtcatcc aaggtccagc
     1621 ccagcatagt cgccactccg cccaggtttt ccagatacac gaattcggtc tgcaaatcgc
     1681 cccgctcgta cgcaccgcgg atgaccactt gtggggcccc ctccgcggtc gggctggctg
     1741 accggtcccc cggctctggg acggcggtgg ccgatccttc tacttgcggg ataaccaata
     1801 cctggccgac cgccagagca tcgggatttg ccagtccatt cgccgccatc aagctttcca
     1861 ttgagatatt gagttccaag gcgatcgcac cgagggaatc tcccggacca accgtgtagg
     1921 ttacgggggt cggggaggga ggcagggtcg cggtcgcggt cgggatggcg cttgcgagcc
     1981 gggccgtgag atcaaaagtc ggcgtcggcg ggaggctggg cagtttcgga cctcggcccc
     2041 agactgccag gaccagcagg atcgtgaggg tcgagaccaa cacgttgagt gcgaggaacg
     2101 ggaggaactg cttccaggtc gcccgccgga cgccacccat agcggcggac tatagcatag
     2161 gagccttgcc aggagtaact tggtcgacgg agcgattttt ataggccttt aatgccgaaa
     2221 ctggcccggc ctatgataag atgccaatag gactggctcg tttacgggct ggtcttaggg
     2281 gagtaagcct accgagcacg tgttccccct acacggacgg tagtgccccg aaactttttg
     2341 aaccgccgga tgcacccccc acattcggcg gttcgcttgt ctggcaagaa tacgcggatg
     2401 ggatcgactg tctgtcggca ggtccatttt ggcacccggt cgaggggacc ctctgttttt
     2461 tcctaaatgc tggagaaggc taccaacaga gccgagatat tgtccggccc gcctgccgcg
     2521 ttagccgccc gaatcagttc ctcacaggcc tcgttgggct cggcgtgcaa atccagcacc
     2581 gacctcactt cgtccatcga tatctcgccc cacagcccgt cgctacacag aagcaaataa
     2641 ccgccccgcg ggaccggata gctgttcact tcgactacca tgttcgaacc ttggccaact
     2701 gcattccaca gctggttgcg cttcgggtgg tctagtgctt cctccggtgt gatttgcccg
     2761 atctcgacca tacgctgcga ataggagtgg tcccgggtca gcagcttgag cactccgttc
     2821 tcgaccaaat aggcgcggct atcaccgaca tggccgattg tcatctggtg gttcataaga
     2881 atcgccacgg tcaatgtggt aaccccgcca ttcgccttct cccgaacagc ctcgttggcg
     2941 cgggcaaagg cctggcgggc gagctcgtcg agctgaacat tgcggctctc cgcctcctga
     3001 gcgagacccg ccagcaccgc gtgctccatc aggctccgca cgaccgcttt caccgcgatc
     3061 gaactcgcca actccccgtg gccgagcccg cccgcgccgt cagccacaca gaacagcccg
     3121 aaatccggca ccacctcctc cccgccggag ttcccataca tcatgaacag ggtgtcttca
     3181 ttcgactcgc gctccagccc cacggactgg gcgctgccgg ccgacagact ctctcgcatt
     3241 ctgacggcgg caatctgctc caagctctcc ggagtaagcg gggcggtgtc ggccagatcc
     3301 ggccctggct ctaccgcctt aggcggagtc agtccgcgca gcctagtcaa cacccgctgc
     3361 cagatgccgg gctttggatc gtttgagttc tcagtcatga gccaaactat tctcgcacac
     3421 tcagatgcaa actccaaact ctacttgtct cctactaggc gcactgagcg acccgcctcg
     3481 cggcgggtcg ctcgatgggc ccggcaggac tcgaacctgc gaccaaccgg ttatgagccg
     3541 gccgctctaa cctctgagct acgggccc
//
LOCUS       DATDPQ010000183         5059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2424169, whole genome shotgun sequence.
ACCESSION   DATDPQ010000183 DATDPQ010000000
VERSION     DATDPQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424169"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VI520_04310"
     CDS             complement(<1..473)
                     /locus_tag="VI520_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HLF37140.1"
                     /translation="MTETTPARVRYAPSPTGRFHIGGARTALYNYLLARQTGGQFIIR
                     IEDTDRKRFVPGSEDEILESLDWLGLRWDEGPDLGGTYGPYRQSERVELYQQHAAQLV
                     EGGFAYFCFCSPQRLSTLRQEQERRKQPPHYDRLCRRLAVSEAAARVQAGESHVVR"
     gene            complement(502..1656)
                     /locus_tag="VI520_04315"
     CDS             complement(502..1656)
                     /locus_tag="VI520_04315"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLF37141.1"
                     /translation="MQASARIWRHFDTWLLGAVGVLTIAGVALIRSAVAGNPELIELN
                     VVGRQAVYGLVSLVVLFAVAAIDYRLWSALSRLLYGFMVLMLGLIPIAGVAGFGAARW
                     FEVATLTVQPSELAKILTILVLADYLGRNRPETLGTLLKSFALVGLPALLILGQPDLS
                     TAVVLLVIWLAMVWAAGARLRYLVIILGLIALAALLVAPLLAQYLLEGYPQGEDFLFV
                     RNYQMTRVINFLFPHPEESFGASYNVEQALISIGSGGLLGEGYGNGTQVQLRFLKVRH
                     TDFLFSSMSQEFGFVGATVTVLLLMFVVFRCLRATRLARDRYGSLICYGVAILLLFQA
                     SFNVGMNLNLLPVSGLPLPFLSYGGSSLLTSLLGIGLVESVMLRHKQIEL"
     gene            complement(1660..1920)
                     /gene="minE"
                     /locus_tag="VI520_04320"
     CDS             complement(1660..1920)
                     /gene="minE"
                     /locus_tag="VI520_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HLF37142.1"
                     /translation="MILQWLRGRERGSARTAKERLQFVLVHDRAGLSASTLEALKDEL
                     IAAISRHVEIDREGVSVSLTKDRRRQRLVADIPLAERRTAAH"
     gene            complement(1917..2726)
                     /gene="minD"
                     /locus_tag="VI520_04325"
     CDS             complement(1917..2726)
                     /gene="minD"
                     /locus_tag="VI520_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HLF37143.1"
                     /translation="MGAKVVTVTSGKGGVGKTTTTANIAVALAAAGHRVVCIDADIGL
                     RNLDLVMGLENRIVYDLVDVVEGRCKLRQAMIKDKRVPNLYLIPAAQTRDKTAITPED
                     MIEVTAQLRRDHDWILIDSPAGIEGGFRSALAPADIVLIVTNPEVSAVRDADRIIGLT
                     EAEEKDPPKLILNRVRPDMIRRGEMLKAEDVLEVLAIELIGVVPEDEQVLTASNRGTP
                     LALDGRSRAGRAFQAIARRLMGETVPLEEFATEDGFLRRLSRKVRTGGQPA"
     gene            complement(2748..3452)
                     /gene="minC"
                     /locus_tag="VI520_04330"
     CDS             complement(2748..3452)
                     /gene="minC"
                     /locus_tag="VI520_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HLF37144.1"
                     /translation="MLKSPPVTTMGSSLALKGIGDGLLVTVPEGDWQTVRPSLLQAID
                     ERADFFRGARLVLQVADRDLGAAELGGLRDELQQREVALAAILSSSAGTRSAGANLGL
                     ALEIPRPELGENGDRDPVSFELEGDQAVLIERTLRSGQKIRHPGHVVILGDVNPGAEV
                     IAGGNVLVWGKLRGVVHAGATGNERSVVCALDLSPTQLRIASHITTSPDRRGNPRPEK
                     AFVKDGSLVAEAWNTN"
     gene            complement(3486..>5059)
                     /locus_tag="VI520_04335"
     CDS             complement(3486..>5059)
                     /locus_tag="VI520_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLF37145.1"
                     /translation="RQYPTGILTSAVIGYLGPIPAAEVDFWESLGFVADRDKVGYGGI
                     EFVYQDILAGTNGLKLVEKDVAGSQLHEVGAGNPPVPGSSLLLTIDTRLQAIATSALE
                     KRMEFINRNAGDVKTTIGALIAMNPQTGEILAMVSIPSYENNRLARFIPEYYFRQLSE
                     DASKPLTNQAIQSSYPPGSSFKMVTALGALNEGVIDPNRTLSDPGLITIQNQYFPNDP
                     GLAKDFVCWKEDGHGNINFVQAIAFSCNVFFYKIGGGFENEVVGEGLGIDNLNIYARA
                     LGYGNLLGIDLPGEEDGLIPNPDWKRLNRGESWSTGDTYISVVGQGFVGATPLQVLTS
                     IATLANGGRVMWPHVVRDVLDGEGNLVQRYEPCVLWDIGDGVLIPPSAIAADCPSLEP
                     EVREQVIAEHLDSPDIVLGPGVLDLVQEGMRLVVEDEEGTAHDYAQLEGISSGGKTGT
                     GEFCDDTAFRRGLCIPGNWPTHSWYTAFAPFENPEIVVVAFVYNGGEGAVTSGPIVQQ
                     VLEAYFQLKAIDAAG"
BASE COUNT          984 a         1616 c         1507 g          952 t
ORIGIN      
        1 cgaaccacgt gcgattcgcc ggcctggact cgggccgcag cctccgagac cgccagccga
       61 cggcagagcc ggtcgtagtg cggtggttgt tttcgacgct cctgttcttg gcgcagggta
      121 ctcaatcgtt gtggggagca gaaacagaaa taggcgaagc cgccctcaac caactgcgct
      181 gcatgctgct gatacagttc gactcgctca gattgccgat agggaccgta ggttcccccg
      241 aggtcgggac cttcgtccca gcgcagtccg agccagtcga gcgactcaag gatttcgtcc
      301 tcggaaccgg gaacgaaccg tttccgatcg gtatcttcaa tccgaatgat gaattgcccg
      361 ccggtttgac gagccagcag atagttgtac agggcggtcc gggctccacc gatgtggaac
      421 ctcccggtag gtgagggcgc gtaacggacc cgggccggcg ttgtttcagt cacttggctg
      481 gataaggaat cggcgggtgg gctagagctc gatctgtttg tggcgcagca tcacactctc
      541 aaccagaccg atcccgagca ggctggtcag cagggaggac cctccgtagg atagaaaggg
      601 caggggcaac ccggaaacgg gcagcaggtt caggttcatc ccgacattga aggaggcctg
      661 gaagagcagc aagatcgcga caccgtaaca gatcagcgag ccgtaccgat cccgcgcgag
      721 tcgggtggcc cgcaggcagc ggaatacaac aaacatcagc agcagtacgg tcacggtcgc
      781 ccccacgaag ccaaactcct gagacatcga ggagaagagg aagtcggtgt ggcgaacttt
      841 aagaaagcgc aactgaacct gggtcccgtt gccatatccc tctccgagca atcccccaga
      901 gccgatcgag atcaaggctt gttcgacgtt gtagctggcc ccgaaactct cttcggggtg
      961 cgggaagagg aaattgatga ctcgggtcat ctgatagttc cgcacgaaga ggaaatcttc
     1021 accttgcggg tagccttcca gtaagtattg agccagcagc ggagcaacca gcagcgcggc
     1081 cagcgcaatc agccccagga tgatcaccag gtatcgcagc cgagcgccgg ccgcccagac
     1141 cattgcgagc cagatcacca gcaggacgac tgccgtgctt agatcgggct ggccaaggat
     1201 cagcaatgct ggaagcccga ccagggcaaa gctcttgagc agggtgccga gcgtctctgg
     1261 gcgattgcga cccaggtaat cggccagcac caggattgtc agaatcttgg ccagttcgga
     1321 gggctgaacg gtcagggtgg ccacctcgaa ccagcgtgcc gcgccgaacc ctgccacgcc
     1381 tgcgattggg atcaacccaa gcatcagtac catgaatccg tacaaaagcc tggaaagcgc
     1441 agaccagagc cggtagtcga tcgccgcgac cgcaaacagg acgaccaggc taaccaggcc
     1501 gtagaccgcc tggcgaccga ctacgttcag ttcgatgagc tccgggtttc cggcaaccgc
     1561 cgatcgaatc aaagcaaccc cggcgatcgt cagtaccccg accgccccga gcagccaggt
     1621 gtcgaaatgg cgccagatcc gtgccgaagc ttgcatccgc tagtgtgcgg cggtcctccg
     1681 ctctgccagc gggatgtcgg cgaccaagcg ctggcgccgg cgatccttgg tgagcgagac
     1741 ggaaactccc tctcgatcga tctccacatg ccgggagatg gctgcgatca attcatcttt
     1801 cagtgcctcg agggtgctgg cactcagtcc ggctcgatcg tggaccagca cgaactgcag
     1861 ccgctccttc gccgtccgcg ccgatccacg ctctcgtcca cgtaaccatt gtaggatcat
     1921 gccggctgtc ctcccgtccg tactttgcgg gatagtcgcc gcaggaatcc atcctcggtc
     1981 gcgaactcct ctagcggaac tgtctctccc atcaggcgcc gggcgatggc ctgaaacgcc
     2041 cgaccggccc gactgcgtcc gtcgagcgca agcggggtac ctcggttgga ggcggtcaac
     2101 acctgctcgt cctcgggaac tactccgatc agttcgatgg ccagcacctc aagcacatct
     2161 tccgctttaa gcatctcacc gcggcggatc atgtccggtc tgacccgatt caggatcagt
     2221 ttaggaggat ccttttcctc ggcctcggtc agcccgatga tccggtcggc atcccgcacc
     2281 gcagagacct ccggattcgt gacgatcagc acgatgtcgg ccggggccaa tgcgctgcgg
     2341 aacccgccct cgatcccggc cggcgagtcg atcaagatcc aatcgtggtc ccgccgcagt
     2401 tgagcggtca cttcgatcat atcctccggc gtgatagcgg tcttgtcgcg ggtctgggcc
     2461 gccgggatca gatacagatt gggtacccgc ttgtccttga tcatggcctg ccgcagcttg
     2521 cagcgccctt ctacgacgtc taccagatcg tacacgattc gattctctag acccatcact
     2581 aaatccaaat tcctcaaccc gatatcagca tcgatgcaca ccacccgatg cccggccgcg
     2641 gccaatgcta ccgcgatgtt cgcagtagtg gtcgtcttgc cgactccgcc cttccctgag
     2701 gtcacggtta cgacctttgc gcccatcctt gcctcactcc ctcctgttca gttcgtgttc
     2761 caggcttctg ctaccaggct tccgtccttc acgaaggcct tctcaggccg cgggtttccc
     2821 ctgcgatccg gtgaagtggt gatgtgactg gcgatccgaa gctgagtggg cgacaggtcc
     2881 agcgcacaga ccactgatcg ctcgttgccg gtggcgccgg cgtgcacgac ccctctcagc
     2941 ttgccccaca ccaacacatt cccgccggcg ataacctccg cgccgggatt gacgtctccc
     3001 aaaatcacca catggccggg gtgcctgatt ttctggccgg accgaagcgt ccgctcgatc
     3061 aatacggcct ggtccccctc gagctcaaat gaaaccggat cacgatcacc gttttcgccg
     3121 agctccggcc gggggatttc gagcgccagg cctagattgg ccccggccga acgggtcccg
     3181 gcggaggagc tgaggatcgc cgccagggcc acctcccgct gttgcagttc gtcccgcaac
     3241 ccgccgagct ccgcagcccc taggtcgcga tcggccactt gaagcaccag gcgtgccccg
     3301 cgaaagaaat cggcgcgctc gtcgatcgcc tgcagcaggg acggccggac ggtttgccag
     3361 tcgccttcgg ggaccgtcac cagcaatcca tcgccgatgc ctttgagcgc gagcgagctg
     3421 cccattgtgg taacgggcgg cgattttagc attcaggtga ctctggcgcc agtcgggggg
     3481 accgctcaac cggcggcgtc gatcgccttg agctggaagt aagcttccaa cacctgctgg
     3541 acgatcgggc cggaggtgac tgctccctcg ccaccgttgt acacgaaggc caccaccacg
     3601 atttcggggt tctcaaaggg agcgaatgcc gtgtaccagg aatgggtggg ccagttcccg
     3661 gggatgcaga ggccgcggcg gaaggcggta tcgtcgcaga attcgccggt gccggtcttc
     3721 ccgccggagg agatcccctc cagctgcgcg taatcgtggg ctgttccctc ttcatcctca
     3781 accaccagtc gcatcccctc ctggaccaga tccagcaccc ccggaccgag cacgatgtcc
     3841 ggggaatcca ggtgctctgc gatgacttgt tcccgcactt ccggctccaa gcttgggcag
     3901 tcggcggcga ttgccgaggg cgggatcaag accccgtcgc cgatgtccca caatacgcag
     3961 ggctcatagc gctggacgag attgccctcg ccgtccaaga catcccgaac cacgtggggc
     4021 cacatgactc tgccgccatt ggcgagcgtg gctatcgagg tgagaacctg aagcggggtc
     4081 gctccgacga atccttgtcc gacgacgctg atataggtgt cgccggtcga ccagctctct
     4141 ccccggttca atcgcttcca atccggattg gggatcaggc cgtcttcctc gcctgggaga
     4201 tcgattccaa gcagattccc gtatccgagg gcacgggcat agatattcag attgtcgatc
     4261 ccgaggccct cgccgaccac ctcattctcg aaaccgccgc cgatcttgta gaagaagacg
     4321 ttgcaggaga aggcaatggc ctgcacgaaa ttgatgtttc cgtgcccgtc ctccttccag
     4381 cacacgaagt ctttcgccag gcccgggtca ttcgggaagt actggttctg aatcgtgatc
     4441 agtccgggat cggagagagt gcgattcggg tcgatgactc cttcgttcag cgctccaagg
     4501 gcagtaacca ttttgaagga ggatccgggc ggatagctcg actggatcgc ctgattggtg
     4561 agcggcttgc tggcgtcctc ggagagctgg cggaagtagt actcaggaat gaagcgggcg
     4621 aggcggttgt tctcgtagct cggaatggag accatcgcca agatctcgcc cgtctgagga
     4681 ttcattgcaa tcaaggcacc gatggtggtc ttcacgtcgc cggcgttgcg gttgataaac
     4741 tccatccttt tctccagcgc actggtcgcg atcgcctgca gccgggtatc gatggttaac
     4801 agcaggctgc ttccgggcac cggcggattc cccgctccca cttcgtgcag ctgtgacccg
     4861 gccacatcct tctcgaccag cttcagtcca ttggtgccgg ccaagatgtc ctgatagacg
     4921 aattcaatcc cgccgtagcc gactttgtca cggtcggcga cgaaccccag gctttcccag
     4981 aaatcgacct cggcggccgg gatgggcccc aggtagccga tgacggcaga ggtcaggatg
     5041 ccggtcgggt attgccgga
//
LOCUS       DATDPQ010000184         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2185320, whole genome shotgun sequence.
ACCESSION   DATDPQ010000184 DATDPQ010000000
VERSION     DATDPQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185320"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VI520_04340"
     CDS             complement(<1..288)
                     /locus_tag="VI520_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HLF37146.1"
                     /translation="MRPNRPVGFAGYGAYIPQFRLPASEVARIWTGGQGGLPIKEKAV
                     AGLDEDVITMSIEAARNALARSGVSASEIRAVWVGSESHPYAVKPTSTVVAE"
     gene            complement(328..1602)
                     /locus_tag="VI520_04345"
     CDS             complement(328..1602)
                     /locus_tag="VI520_04345"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HLF37147.1"
                     /translation="MTDSRLAGFYNLDLAQRQARLSASTGLDLAPIFGSDGLSAAQAD
                     AMIENVVGVFGLPLGIAANFVVNGKDVLVPMAIEEASVVAGASHMAKLVRESGGFRAE
                     ADPSHMIGQIQLVDLADPAAAERALLAAESEILTACNLANPRLVELGGGSRGIEVRRL
                     EPSSSPPMLVVHLIYDTVDSMGANSVNTVLESVAPMIEAISGGRAHLRILSNLADRRL
                     ARASCAIPPQRLRSEQYDGEQVRDGIIEAWAFAAADPYRAATHNKGIMNGIDAVVIAT
                     GNDWRAVEAGAHAFAARSGRYAPLSRWSTDDQGQLVGELELPLALGTVGGATRSHPAA
                     QACLALMQARSARELAGIVAAVGLAQNLAALRALATDGIQRGHMGLHARQLAMSAGAS
                     GPQIEQIARRMVEEGVIALERAQELMQTSSGS"
     gene            complement(1673..1888)
                     /locus_tag="VI520_04350"
     CDS             complement(1673..1888)
                     /locus_tag="VI520_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37148.1"
                     /translation="MRTRRIVLWVISLGFGVAGTVGTVLIFGSDFNRFWTNFLFKGDL
                     PLLLLVGYGSLAFIWLDLILQTQYLKR"
     gene            complement(2028..>2269)
                     /locus_tag="VI520_04355"
     CDS             complement(2028..>2269)
                     /locus_tag="VI520_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF37149.1"
                     /translation="SVPDLILTDLMMPEIDGLSLIRRLRETPIWAQIPVVMVSAHSEP
                     EIQESAKQAGAAGFIAKPFSASELRATVRAHLVEN"
BASE COUNT          415 a          723 c          733 g          398 t
ORIGIN      
        1 ctccgcgacc acggtcgagg tcggtttgac cgcgtacggg tgcgactccg aaccgaccca
       61 gacggcccgg atctcggagg cggagacccc ggatcgggcc agcgcgttgc gggcggcctc
      121 gatcgacatc gtgatcacgt cctcatccag gccggctact gctttttcct tgatcgggag
      181 tccgccctga ccaccggtcc agatgcgggc cacctcgctg gctggcagac ggaactgcgg
      241 gatgtaggcg ccgtagccgg cgaagcccac cggccggttc ggtctcatca gatgattttc
      301 actcatgacg gatagttaca cctcgtgtca gctgcccgaa ctggtttgca ttagttcctg
      361 agctcgttcc aacgcgataa cgccttcttc aaccatccga cgtgcaatct gctcgatctg
      421 gggtccgctg gcgccggccg acatggcgag ctggcgggcg tgcagcccca tgtggccgcg
      481 ttgaatgccg tcagtcgcca gcgctcgcag cgcggccagg ttctgggcca gccccaccgc
      541 ggccacgatt ccggccaact cccgcgcgct gcgggcctgc atgagagcca aacaggcctg
      601 ggcggccggg tgggatcggg ttgcgccgcc tacggttccc agggccaggg ggagctcgag
      661 ctcgccgacc aactgcccct gatcatcggt ggaccaccgg ctcaacggcg cgtagcgccc
      721 gctgcgcgca gcaaaagcgt gcgccccggc ttccaccgcc cgccaatcgt ttccggtggc
      781 aatcaccacc gcgtcgatcc cgttcatgat tcctttgttg tgagtcgccg cccgataggg
      841 atcggcggcc gcgaaggccc aggcctcgat gatcccgtcc cgaacctgct cgccgtcgta
      901 ctgctcggag cgcaaccgct gcggcggaat cgcgcagctc gctctggcca gtctccggtc
      961 ggcgaggttc gagagaattc gcaggtgggc ccggccgccg ctgatcgctt caatcatcgg
     1021 ggcaacgctc tccagcaccg tgttgaccga gttggcgccc atcgaatcga ccgtgtcata
     1081 gatcaggtgc actaccagca tcggagggga ggaggacggc tcaagtcggc ggacctctat
     1141 cccgcgtgat ccgcctccca attcgaccag ccgcgggttg gccaaattgc aggcggtcaa
     1201 gatctcgctc tcggccgcca gcagggcccg ctccgcggcg gccggatcgg ccaggtccac
     1261 cagctggatc tgcccgatca tgtgcgaggg atcggcttcg gcccgaaatc cgccggactc
     1321 acggaccaat ttggccatgt gggaggcccc ggccacaacc gaagcctctt cgatggccat
     1381 gggaaccaag acgtccttcc cgttgacgac gaagttggcc gcaatcccga gcggcagccc
     1441 gaagactcct acgacattct caatcatggc gtccgcctgg gccgcgctca gcccatcgga
     1501 tccgaagatc ggggcgaggt cgaggccggt ggaggcggag agccgcgcct ggcgctgggc
     1561 caggtccaag ttgtagaaac ccgccagacg cgaatctgtc attgatggtc ctgcaccgat
     1621 tgcttatatc ggctgccggc tggcaaaaac tatcaggaag cggggcggag gactagcgct
     1681 tgaggtactg ggtctgcaaa atcaggtcga gccagatgaa ggccaggctg ccgtacccga
     1741 ccagcagcag cagcggcagg tctcccttga acaggaagtt ggtccaaaag cgattgaagt
     1801 cggagccgaa aataagcaca gtgccgaccg tgcccgccac cccaaagccg agcgaaatca
     1861 cccacagaac gatgcgacgg gttctcatag gtcctttgga aacggaacgc ggccgattgt
     1921 aaatgggccg cgggattggg tcaagcggag ggattgaccc gggtgggtgt aaagggcttt
     1981 aggtgatcgt taaggtgata gccgaagtaa tcgcaggtgt catcggttca attctctacg
     2041 agatgggccc ggaccgtcgc tcgcagctcg ctggccgaga acggtttggc gatgaagccg
     2101 gccgcgccgg cctgcttggc ggactcctga atctcgggtt ctgagtgggc gctgaccatt
     2161 acgaccggga tctgggccca aattggagtt tcccggaggc gtcggatcaa actcaggcca
     2221 tcgatttcgg gcatcatcag atcggtgagg atcaggtccg ggacggatt
//
LOCUS       DATDPQ010000185         4007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1656929, whole genome shotgun sequence.
ACCESSION   DATDPQ010000185 DATDPQ010000000
VERSION     DATDPQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656929"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VI520_04360"
     CDS             <1..980
                     /locus_tag="VI520_04360"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF37150.1"
                     /translation="GNTVVIKPSEFTPLATLRLAELAFAHLPPGVVNVVTGYGREAGE
                     ALGRHPEVPVIAFTGSLATGRHIAELAAPMMKKLHLELGGKDPMVIGPDVDLDTAVRA
                     LAYAGLLNAGQVCTSTERVYVAQDRYPEFSEALAEFVGALRLGNGLESTTDIGPMLRE
                     HFREKVEAHVQQAIGAGARLLVGGERPKEFERGFFYRPTVLADVNHSMAVMREETFGP
                     VLPLMPYEDFDQAIELANDSQYGLGASLMSNDPRLVKRFFEEVDAGTIWINDPLTDNY
                     AGPFGGMKMSGVGRELGQEGLDEFYEVKHVHWDMLASPKDYWYPYGTGR"
     gene            complement(977..3142)
                     /locus_tag="VI520_04365"
     CDS             complement(977..3142)
                     /locus_tag="VI520_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HLF37151.1"
                     /translation="MASLAHRLWSDRTPRSELIGKAAVVAAAYFVTGKLSVLLAIPPG
                     IASPVWVPSGIALAAVLRWGYGVGAGVWVASFLVNIGILLESGGTGAVAAVSGIATGS
                     TLQAVAGAFLIRRFAEVPGIFDSVRGTFRFIGAELLACLIAPSFGAGSLYLGGYISNP
                     ALPLTWLTWWLGDSVSVFVVTPIFLISWRPASDRLGEAVGLWSILVAFGILVFEGAVL
                     PAEMVRPPVGYVFILVVIWAAFRMGQSGVVGTSILISAIAIHGTIRGRGGFALPNTNE
                     SLLQLQAFIGLSTLTGLLTAAGLTERRRAEQALREAQAELESRVQERTGELTRSTAAL
                     QAEFAERRRTDDALHESEGMLRGLFEFAPDAIVVTDQAGRIVRINAQTELLFGYGREE
                     LIGRPVEVLLPDRLAARHAQHRKSFLVRPQARSMGVGLELYGRRRDGGEFPVDIMLSP
                     VESREGRTVIAVVRDIAERKRTEEAMRHSRNGLAELYQRLQDLDRLKSKFISDVSHEL
                     RTPIANLALYLDLIETGKPAKRKQYEAALREQIGRLTDLIEEISNLSRLEQDQLTAAF
                     GSVDLNAVVDLVVNEFQSRAKTAGLALSFEPGGDPLLVRAVADQLRQVIANLLTNAIN
                     YTPQGRVWVNTGRADDQVYLQVGDTGMGIEPPDMQHVFDRFYRGRGVSHIPGSGLGLA
                     VVDRIVRIHSGTVEMESRAGEGTTLTVRLPASDSRQPST"
     gene            3417..>4007
                     /locus_tag="VI520_04370"
     CDS             3417..>4007
                     /locus_tag="VI520_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLF37152.1"
                     /translation="MAEISSNPKNVVLPRAASPEAVARLSPVWFRYTDLVVERGEGAY
                     LYDREGKAYLDFTCGIGVTNTGHCHPRVVQAVQAQAAKLLHGQVNIVHHPALLELVEE
                     LGSILPSALDGFFFSNSGAEAIEGALKLARAATGRPNVIVFQGSFHGRTVGTMSLTTS
                     KTIYRAGYQPLMPGVFVAPFPYAYRYGWEPEQTRDWC"
BASE COUNT          771 a         1267 c         1242 g          727 t
ORIGIN      
        1 cgggcaatac ggtggtcatc aaaccctccg agttcacgcc gctggccacg ctgcggctgg
       61 cggaactggc ctttgctcac ctgccgccgg gcgtggtcaa tgtggtgacc ggctacggtc
      121 gggaggccgg ggaagcgttg gggcggcacc cggaggtgcc ggtgatcgcc ttcaccgggt
      181 cgctggccac tggtcgacat atcgccgaat tagccgcccc catgatgaaa aagctacacc
      241 tggagctggg gggcaaagac ccaatggtca tcgggcctga cgtggatttg gacaccgcgg
      301 tgcgcgccct ggcttatgca ggcctgctga acgccggcca ggtgtgcacc agcaccgagc
      361 gggtgtatgt ggctcaggac cgctatcccg agttttcaga ggccctggcc gagttcgtcg
      421 gggcactccg gcttgggaat gggctggaaa gcacgaccga tatcggccca atgctgcggg
      481 agcactttcg ggagaaggtc gaggcccacg tccagcaggc gatcggggcg ggcgcccggc
      541 tgttggtggg cggcgagcgg ccgaaggaat tcgagcgggg cttcttctac cggccgacgg
      601 ttctggccga cgtgaaccat tcgatggccg tgatgcggga agagaccttc ggaccggtgc
      661 tgccgctcat gccatatgaa gacttcgacc aggcgatcga gctggccaat gacagccaat
      721 acggcctggg ggcctcgctg atgagcaacg acccccggct ggtgaagcgg tttttcgaag
      781 aggtggatgc cggcacgatc tggatcaacg atccactgac cgacaactac gccgggccgt
      841 ttggcgggat gaagatgagc ggggttggcc gtgagctcgg ccaagaaggg ctcgatgagt
      901 tctacgaggt caagcatgta cattgggaca tgctggcctc gcccaaggac tattggtatc
      961 cctatggcac tgggcgctag gtcgaaggct ggcgggaatc ggaagccggc agtcgcacgg
     1021 ttaaggtggt gccctcccct gcccggctct ccatctcgac agtgccgctg tggatgcgga
     1081 cgatccggtc gaccacagcc agccccagcc cgctaccggg aatgtggctg acgccccgac
     1141 ctcgataaaa acgatcgaac acatgttgca tatctggtgg ctcaatcccc atacctgtgt
     1201 ccccgacttg taggtaaacc tgatcatcgg cccggccggt atttacccaa acccgaccct
     1261 gaggcgtgta gttgattgcg tttgtcaaca gattcgcgat gacttgcctc aactggtcgg
     1321 ctacggctcg cacgagcaac gggtcaccgc cgggttcgaa gctcagtgcc agcccggccg
     1381 tcttggcccg ggattgaaac tcgttgacaa ccaggtcgac gaccgcgttc aaatccacac
     1441 tcccgaacgc tgccgttagc tggtcttgtt ccaggcgcga gagattcgaa atctcctcga
     1501 tgagatcggt caatctgcca atttgctctc tgagggccgc ttcgtattgc tttcgcttcg
     1561 ccggcttgcc ggtctctatc aaatccaaat acagggccag gttagcgatc ggcgtgcgca
     1621 gctcgtggga gacatcgctg atgaacttag acttcaggcg gtcgaggtcc tggagccgct
     1681 gatacagttc ggcaagcccg ttgcggctgt ggcgcatcgc ttcctcggta cgtttgcgct
     1741 cggcgatatc gcgcacgacg gcgatcacgg tccttccctc tctggattcc accggactca
     1801 gcatgatatc cacagggaat tcgcccccgt cccggcgcct gccgtacagc tccaagccga
     1861 ctcccattga acgcgcttgc ggcctgacga gaaagctctt gcgatgctgg gcgtgccgcg
     1921 ccgcgagtcg atccggcagc aagacttcga ctggtcggcc gattagctcc tcccggccgt
     1981 aaccaaacag cagctcggtc tgggcgttga tccgcacgat gcgccccgcc tggtcggtga
     2041 cgacaatggc atctggcgcg aattcaaaca gcccgcgcag catgccttcg ctctcatgca
     2101 gtgcatcatc ggtgcgtcgc cgctcggcga attcggcctg taaggcggcg gtcgaccggg
     2161 tgagctcacc agtcctttcc tgaactcggc tctccagctc ggcctgcgct tcgcgcagcg
     2221 cctgttccgc ccgccggcgc tccgtgagcc cggccgccgt gagcagaccc gtcaatgtgc
     2281 ttagcccgat gaacgcttgc agctggagca aagactcatt ggtattcggc agcgcaaatc
     2341 cgcctctgcc ccgaatcgtt ccgtgaatgg cgatggcgct gatcaggatc gaagtcccca
     2401 cgaccccgct ttgccccatc cggaaggccg cccaaatcac gaccaggatg aaaacgtagc
     2461 cgaccggtgg ccgaaccatc tcggccggta atacggctcc ttcgaagacc aggattccga
     2521 acgcaaccag gatcgaccat aacccaaccg cttctcccag ccggtcgctt gccgggcgcc
     2581 acgaaatcag gaagatgggc gtcacaacaa aaacgctgac cgagtcgccc agccaccagg
     2641 tcaaccatgt caatggcaag gcagggtttg agatgtatcc tcccaagtaa aggcttcccg
     2701 cgccgaagct gggggcgatt aggcaagcca gcaactctgc cccaatgaag cggaacgtgc
     2761 cccggacgct gtcgaatatc ccaggcactt cggcgaagcg tcgaatcaag aacgccccgg
     2821 caaccgcttg cagggtcgat ccggtagcaa tcccgctgac ggctgccacc gcgcccgtac
     2881 cgccgctctc aaggagaatc ccaatgttta ctaagaacga agcgacccaa acaccggctc
     2941 caactccgta tccccagcgt agaaccgcgg ccagcgcaat cccggatgga acccacaccg
     3001 gggatgcaat gcccggcggg atggccagca gcaccgaaag tttgccggtg acaaagtaag
     3061 cggccgccac cacggctgcc ttgccgatca gttcggaccg gggcgttcga tcagaccaga
     3121 ggcggtgcgc taaggaggcc atatcaagtt cggctttctt tcagccgcag gttgaatggc
     3181 ggcggctgag cgaagccgtt ggcgcggcag tgtagggcca tcggcgacat ttggcgagga
     3241 ggtctctgcc ggcggctgag cagagtatac agcccgccca tgatcgcaca atgggtccac
     3301 cggcagggca gcaggtcgct gactcatctg cgccgcacac gggcatccca ggagatggtt
     3361 ggcaatagct cctcatcctc gaccagcaat agcaggtgga tgataattgg cctgaagtgg
     3421 ctgagatttc atccaacccg aagaatgtag tcctgccccg agctgcgagc ccagaggccg
     3481 tagctcggct ctctccggta tggttccgct acaccgacct ggtcgttgaa cgcggggagg
     3541 gagcttacct ctacgatcgg gaggggaagg cctacctgga tttcacctgc gggattggcg
     3601 tcaccaacac tggccactgc catcctcgag tggttcaggc ggtccaggcc caggccgcca
     3661 agctcctgca cgggcaggtc aacatcgtcc accacccggc tctgctcgag ctggtcgagg
     3721 agctgggaag catcctgccg tccgcgttgg acggattctt tttcagcaac agcggcgcgg
     3781 aagcgatcga gggcgccctc aagctggcgc gagcggctac gggtcgcccg aatgtcattg
     3841 tgttccaggg atccttccac ggccggacgg tgggcacgat gtcgctgacc accagcaaga
     3901 ccatctaccg ggccggctac cagccgctga tgcccggagt cttcgtggct ccgttcccct
     3961 acgcctaccg ctacggctgg gagccggagc agacccggga ctggtgt
//
LOCUS       DATDPQ010000186         4509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2699410, whole genome shotgun sequence.
ACCESSION   DATDPQ010000186 DATDPQ010000000
VERSION     DATDPQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2699410"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VI520_04375"
     CDS             complement(<1..1350)
                     /locus_tag="VI520_04375"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37153.1"
                     /translation="MSEARKTRKRTTGKGNQRMATKPPTPVKPSRDSKSPAPSQFRAQ
                     IRKLQQQAVEAQREFSYDQAIELFNEALERSSQHPGLSEAGVEYGLLAGRAACYRRIG
                     SSEAASADFDSMARLAKREGDLDREIEALNNLAQAVINRGDFGVAERKAKSALRLARA
                     AGNPKLEAESLVSLSEWKRAVGEPGEAQTLLEKALALFRQLDDRAGEASSLRRLGRLS
                     IAIGEPAKSHGYLNEALLLYRALGDLEGEGHSLNSLALGSSNHYETRTLGEQALAIFQ
                     TIGDVDGQSTMFNNLGLMYANLGLYRTASEYTSRAVEMAKRAQARLLAATYLESLGRS
                     ELDYGDLDSAQATLQEGLDLSIEIGHVHNESYYRLGLGRLALARGQVDAARKEVQTAC
                     DHFRALGDPSVLAVSLSWLGYIHLQTGDWEAAHRFSSEAVAQMEALGDISWDYPPQDV
                     "
     gene            complement(1366..2625)
                     /locus_tag="VI520_04380"
     CDS             complement(1366..2625)
                     /locus_tag="VI520_04380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HLF37154.1"
                     /translation="MKQETLRRVPLFSGLPDDELATLATGLVDRPIPAGTLLFREGDP
                     GGRSFFILDGEVEIVKSLGTAEERLLAVRGPGSSLGEMELFSQGHLRTASVRAKSRLQ
                     VVEMSRADLENLLRRQPALAFEIVRTLSMRLDESESQTILDLREKNRLLQQAYDELKA
                     AQAQLIEKEKLERELEVARQIQVGILPRSLPELPGLDFGARMVPTSAVGGDFYDVIQL
                     GNGRIGLVVGDVTGHGVPAALLMALTVTLVRVEAPRASSPAEVLFSVNRHLIESNDSG
                     LFVTALYGILDVSTLEFHYARAGHPLPVALDSRANSVMLEQGTGQPLGLFDPLALDEG
                     RLQLDRGGSMLLYTDGVTEAVDGAGEFFGTERLTSALQAAAGFHPERICEALWLALTA
                     YQGTMPQDDDVTLVAVGIRNGTDGHPI"
     gene            2734..3852
                     /locus_tag="VI520_04385"
     CDS             2734..3852
                     /locus_tag="VI520_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF37155.1"
                     /translation="MRSGSVSFGAVGLTLGLALAACSFTQLVAPSDLALGGPAAQAES
                     TATPLPSPRPSFTPDPTPTASPTPTPTLHPLSIDWLRSQEFPGSEITIEQTLEPGANY
                     SRYLASYRSEGLRIYALLTIPFGEMPETGWPAVIFNHGFIPPDEYRTTERYVAYVDGF
                     ARNGYIVFRSDYRGHAESEGVARGGYGSPDYTIDVLNAVASVKAFSQADPARIGMWGH
                     SMGGYITLRSLVVSEDIRAGVIWAGVVASYPDLLTRWRRPATGGLQPTPRPGSWRSSL
                     AETFGSPDENPSFWGSISANSYLADLSGPVQLHHGTADTSVPLEFSAILYEQILEIGG
                     TVEYYEYPEDNHNLSNYFTTAMQRSIQFFDRYVKGPAG"
     gene            complement(3849..>4509)
                     /locus_tag="VI520_04390"
     CDS             complement(3849..>4509)
                     /locus_tag="VI520_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLF37156.1"
                     /translation="LVIAHFWLQDERITLPRTLGLLIGFGGVAILVGQNFDLERILEG
                     NALGQLAVLAASASYATGLTLTRKRFRGQPPVSQATLVVLLSDLMLWVGALVIESPLR
                     LPSLSLTWIALLWLGFLGTGLAYLLYFYLINEWGSTRTSVVTYTMPVIGVILGVVFLG
                     ESLSVSLILAVGLVLTGIGFVNWKHRRTETAPPTEPESVTGPGGLGGLARMPDRVEGE
                     S"
BASE COUNT          844 a         1441 c         1365 g          859 t
ORIGIN      
        1 gacgtcctga ggcgggtaat cccaactgat gtcgcccagc gcttccattt gggccaccgc
       61 ctcggaactg aaacgatggg cggcttccca gtcgccggtc tgcagatgga tgtaaccgag
      121 ccaggagagc gagacggcca ggaccgaagg gtcgccgagg gctctgaagt gatcgcaggc
      181 cgtttgaact tccttgcgcg ccgcgtcaac ctggccgcgg gcgagcgcca agcggccgag
      241 accgaggcgg tagtaggact cattatgtac atgaccgatt tcaattgaaa gatccagccc
      301 ctcctggagc gtcgcctggg cagaatccag gtctccgtaa tcgagttcac tgcggcccag
      361 gctctcgaga taggtggccg cgagcaggcg agcttgggcc cgcttggcca tctcgaccgc
      421 tcgggaggtg tattcgctgg ctgttcggta aagccccagg ttggcataca tcaacccgag
      481 gttgttgaac atggtgctct ggccatccac gtcgccgatg gtctggaaga tggccagggc
      541 ttgctccccc agggtccgag tttcgtaatg gttgctgctc cccagcgcca gtgagttgag
      601 cgaatggccc tcgccctcca ggtcgccgag agcccggtac agcagcaagg cctcattgag
      661 gtagccatgg gacttggcgg gctccccgat ggcgatgctg agccggccga gcctgcgcag
      721 gctactcgcc tcacctgccc ggtcgtcgag ctggcggaag agggctagtg ccttctccaa
      781 gagagtctgc gcttcgccag gctcaccgac ggcccgtttc cattcactca aactgaccag
      841 gctttcggct tcaagcttgg gattgccggc ggcacgcgcc agtcgcaatg ctgatttggc
      901 tttgcgttcc gcaaccccaa aatcgccccg attgatgacg gcctgggcaa gattgttcag
      961 agcctcgatc tcgcggtcca gatccccctc gcgcttggcc aacctggcca ttgaatcaaa
     1021 gtcggcgctg gccgcttcgc tgctccctat tcgccgatag caggccgctc gcccggccag
     1081 cagtccatac tcgacacccg cctcgctcaa ccccggatgc tggctcgagc gctccaaggc
     1141 ctcgttgaag agttcgatgg cctgatcata ggagaactcc cgctgggcct cgaccgcttg
     1201 ctgctgcaat ttgcgaatct gggcgcgaaa ctggctcggg gcaggagact tggaatctcg
     1261 ggaaggcttc accggcgttg gaggcttggt tgccatcctt tggttaccct tgccggtggt
     1321 ccgcttccga gtttttcggg cttcactcat cgttgcattc tccgcctaga ttgggtgtcc
     1381 atctgtgcca ttcctgatgc caaccgcaac cagagtgacg tcatcgtcct ggggcatcgt
     1441 cccctgataa gcggtgagcg ccagccaaag cgcctcgcag atccgctccg ggtggaagcc
     1501 cgccgccgcc tgcaaagccg aggtcaagcg ctcagtgccg aagaattccc ctgccccatc
     1561 cacggcctct gtcaccccgt cggtgtagag cagcattgaa ccgccccggt caagctgcag
     1621 ccgcccctca tccaaagcca gcggatcgaa taggcccagc ggctgcccag tgccttgctc
     1681 cagcatgacc gaattcgccc gtgaatccaa agccacggga agtggatggc cggcccgggc
     1741 ataatggaat tccagagtac tcacgtctag tatgccgtac agggccgtga caaagagacc
     1801 cgaatcgttg gattcaatca gatggcggtt gactgagaaa agcacttccg cgggcgagct
     1861 tgcgcggggg gcctcgaccc tgaccagggt gacggttagg gccatgagca aggcggccgg
     1921 cacaccgtgc ccggtgacat ccccgacgac cagtccgatc ctcccgttgc ccaactggat
     1981 tacatcgtag aagtcccctc cgaccgcgct ggtgggcacc atccgggccc cgaaatcaag
     2041 gcccggtagt tcgggaagtg agcggggcag gatcccgact tggatctgcc gagcaacctc
     2101 cagctcgcgc tctaacttct ctttctcaat caattgggct tgagcggctt tgagctcgtc
     2161 gtaggcctgc tggagcaggc ggttcttctc gcgaagatca aggattgttt ggctttcgga
     2221 ttcatctaac cgcatgctca gcgtgcggac tatctcgaag gccaaggccg gttggcgccg
     2281 aagcaaattc tctaggtcag cccgactcat ctccacgacc tgcagtcgcg acttggcgcg
     2341 cacactggca gttcgaaggt gtccctggct gaagagttcc atttctccca gcgacgagcc
     2401 cgggccgcgc acggcgagaa ggcgttcctc agctgtgccg agcgacttca caatctcgac
     2461 ctccccgtca agtatgaaaa aagatcggcc gcctggatcc ccctcccgga agagcaacgt
     2521 tcctgctggg attgggcggt ccaccaggcc cgtggccagg gtcgccagtt cgtcatccgg
     2581 cagaccagag aacagtggga ctcgacggag ggtttcctgt ttcatcacag cccggaagga
     2641 aggctgcccc tgggccgccc aatgataatt gacaattgcc cgtagctcct gaggccgtta
     2701 ccggacatgt cgtacaatgg cccgccttac acgatgagaa gcggctcggt gtccttcggt
     2761 gcggtggggt taacccttgg gctggcgctg gcagcctgtt ccttcaccca gctggtggct
     2821 ccttcagact tggccctggg cggacccgcc gcgcaggctg agtccaccgc cacccccctc
     2881 ccgagtcccc ggccgagctt cactccggac ccaactccaa ccgcttcccc gactccgacc
     2941 cccacccttc atccgctctc gattgattgg ctgcgcagcc aagaattccc gggaagcgag
     3001 atcaccatcg agcagaccct cgagcctggg gccaactact cgcggtacct cgcgtcgtac
     3061 cgatctgagg ggctcaggat ctacgcgctg ctgacaatac cgttcgggga gatgcccgaa
     3121 accggatggc cggcagttat cttcaatcac ggattcatcc cccctgacga gtatcggacg
     3181 actgagcgtt acgtagccta tgtggacggc ttcgcccgca atgggtacat cgtgtttcgt
     3241 tctgattacc gcggccatgc ggagtccgag ggtgtggcga gaggggggta cggctcgccg
     3301 gactacacca tcgatgtgtt gaatgcggtg gcgtcggtca aggctttctc gcaggccgat
     3361 cccgctcgga tcggaatgtg gggacactcg atgggcggct acatcacgct gcggtcgttg
     3421 gtggtctcgg aggacattcg ggccggtgtg atctgggcgg gggtggtggc ctcgtatcca
     3481 gatctgctga cccgctggcg gcggcctgcg accggtgggc tgcagcctac tccccgaccg
     3541 gggagctggc ggagttcact ggcggagacg tttggttcgc cggatgagaa tccctcgttc
     3601 tggggttcga tctcggccaa ttcttacctg gccgatctct ccgggccggt ccagcttcat
     3661 cacgggaccg ccgacaccag cgttccgctc gagttttcgg caattctcta cgaacagata
     3721 ctagaaatcg ggggaacggt cgagtactac gagtatccgg aggacaacca caacctgtcg
     3781 aactacttca cgacagccat gcagcggtcg atccaattct tcgaccgtta cgtaaaggga
     3841 ccggccggct aggattcacc ttccacgcgg tccggcatcc gcgccaagcc gcccaggcca
     3901 ccaggccccg tcaccgactc tggctccgtt gggggtgcag tttcggtgcg gcgatgcttc
     3961 cagttgacga acccaattcc ggtcaggacc aggccgactg cgaggatcag actcacagat
     4021 agcgactcgc ccaagaatac gaccccgagg atgactccga tcaccggcat ggtgtaggtc
     4081 accaccgatg tgcgggtcga gccccactcg ttgatcaaat agaagtacag caggtaggcc
     4141 aaaccggtgc ccaagaaccc aagccacaag agggcgatcc aggtcaggga caggctcgga
     4201 agtcgcagtg gcgattcaat gaccagcgca cccacccaca gcatcagatc cgagagcagc
     4261 acgaccaagg tcgcttggga caccggcggc tgaccgcgga accgcttccg ggtgagagtg
     4321 agccccgtgg cgtagctggc ggaggcggcc aggaccgcca gctggccgag cgcgttcccc
     4381 tccaggatcc tttccaagtc gaagttctgc ccgaccagga ttgccactcc accgaagccg
     4441 atcaggagtc ccaatgtgcg tggcagggtg attcgctcgt cctgcagcca gaaatgggcg
     4501 atgaccagg
//
LOCUS       DATDPQ010000187        10893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_862617, whole genome shotgun sequence.
ACCESSION   DATDPQ010000187 DATDPQ010000000
VERSION     DATDPQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862617"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VI520_04395"
     CDS             complement(<1..438)
                     /locus_tag="VI520_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37157.1"
                     /translation="MAGKAMKEVSVLIVDDHPLFREGVRNVLISEEDIKIIGETTSGD
                     EALQLVRDKQPMVALVDVNLPSMNGIQVTRQVVAERIKTAIVLLTAYDDVEQVLHAFK
                     AGAAAYCSKDVEPGKLVDVIRNVARGYFVVGDQVYDQAGLDQWL"
     gene            complement(420..1994)
                     /locus_tag="VI520_04400"
     CDS             complement(420..1994)
                     /locus_tag="VI520_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37158.1"
                     /translation="MRPGPRIARPSAAGLTALALLASAALYLWTAQRTFRLGLPLDDA
                     WIHQTYARNLVELKQWAFLPGEPSAGSTAPLWTGVLAVGYWLRVNPLLFTYAVGMAVL
                     GLVAWLALRWVQALVPERAGWAWTLAVLIGIEWHLVWAGLSGMETLAVAGLAVLVLLA
                     TETGWLGPGRLGMLIGLGVWLRPDGLTLVGVPLGRLLLGERRKIVPGLARLAAGLAAV
                     LVPYLLWQRSLSGAWWPNTAFAKQAEYAALQELPFAIRLAAQAGIPGAWLGVTGLDPG
                     GPLIGVMAALVPGLVLFVIAQLRARRWDRLLGLVWSAAYIGLYAARLPATYQHGRYAL
                     PVLPVWMALAGAGMLGELELRPNHFGRRVLSRAWIALVPIIAGYFWLAGARAYGRDVA
                     IIESEMVETARWVEAHTPEGAVVAAHDIGALGYFGHRTLIDLAGLIDPTVVPILRDEP
                     ALARYLSERGADYLMTFPGWYPKLTAGRVPAYETGARFSPAAGSENMAVYRWEPSSFA
                     PCGGCAILAELWQGRP"
     gene            complement(1991..3595)
                     /locus_tag="VI520_04405"
     CDS             complement(1991..3595)
                     /locus_tag="VI520_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF37159.1"
                     /translation="MPTSTTSKAGTADRTPEPETLHTIRLLLIGLAMSLLAAAWVRQP
                     GYMDADYYMLTARQLARGEGFTEPVLWNYLDDPSQIPHPSHLYWLPLASLVAAGSMTL
                     FGESFRAAQAPFVLIAAVLPLLAGRLAFQLTQDRELATQAGWLAAFSGFFLPFNVTTD
                     VFAAYAVLGAIAFWLMASAMEKRSARRWLAVGAVTGLGSLARADGLLLLAIAVLAVIL
                     SKQNRARGFGALLVGFVLVLGPWWLRNLSAVGSVANPGSLRLLWMLSYDDLFSYPAEL
                     LDFERWRQAGLPTLLAARGSALLWNSARLVAENGLVFLGPFMLLGAIRLWSRRLVRLP
                     IYYLGLLFGVMTIVFPFIGPRGAFFHSSTAAMPVLWALAPVGLRGAIDWAGRKRNWDI
                     GQARRVLGTAAMVLAAGLTIGLVALRLVIPAAAGQGWGAGLQTYSELGERLSGSEGRV
                     AVNNPPGFSLATDREAVVIPNGPPETLHAVVRRYQVDWVVLESNHPSQLDPLYARPGS
                     VPWLRLADQIADPFGRPVYLLEVIQK"
     gene            complement(3546..5096)
                     /locus_tag="VI520_04410"
     CDS             complement(3546..5096)
                     /locus_tag="VI520_04410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF37160.1"
                     /translation="MLESKRAIQTAPKQEPRGLQIGRRVWIPILFVALLARLPGVVTK
                     PIWFDEAFSALLSVKGPAAIVEATTADVHPLGYYLLLWAWGRVFGTTPFALRAFSVLV
                     GLGLVGVAYAAARAMVGPRLAAWFGWLVALSPFQVHFAQEIRMYGLLALELTAAAALY
                     WAAVNQGGWLRWIGFAVLAAAAQYTHSLAAMYLLPMALIPVYRRNWKAVQATLLCGLG
                     SLILYAPWLLRLATQIAGIRQAYWIPIPGPVELVRTLIIYLGGLPIATWALPIVLFSQ
                     VLVIGLGGWALLKGRRSNDPNSAIAGWSVYLAFAPLLLSFLVSQWVPVYLDRALLPAG
                     VAFLFGLAWVLGGDQLPQRFRWTAVAAVALSFGVGLFSYWTYRGFAYAPFDEIVAHIG
                     ENKTEGEIVLHSNKLTAFPSIYYAPELTQAYLADPPGSGSDTLAPATQEVLGLTAQAD
                     APTVIGDSDGVWFVIFRREIDDYAALGLPEHPVLAWLRNNFPDEAVTSFGDADLYHFQ
                     GRHGGSDT"
     gene            complement(5096..7096)
                     /locus_tag="VI520_04415"
     CDS             complement(5096..7096)
                     /locus_tag="VI520_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37161.1"
                     /translation="MTWVVIVTSAYFAVHKPGSPAQLGALARATGSVAGVLMTLSVAT
                     VLGLPWRRALDGLEARSQIALQLGAGMAGLSFLALSMGALGAYHPTVAWGIATLSLPF
                     SLRQWIGMLRERRIGLPSGRANRWLAAFVLGLISLAAVSALAPPTAWDSLVYHLTGPK
                     LYLEAGRLTHDLDLPYLGFPQAGSMVFLWGMLLAGDRVPQLIHLSFGLLTLALLPTVV
                     RRVAPGRGWLAAAILLGVPSAQLLLGLAYVEWITAFAGLACFVLLTTGDDVGPTGESV
                     GTGELARSIGSQRILRIVLAGLFAALALNTKYSAVWMVAGLGLVAYLRRRSTGEVVLF
                     AGSVSTFLIPFFMKNWLLTSNPVYPFFFSGRFWDAYRAAWFSRAGTGLGAGQLLVAPW
                     EATIWGLEGGYFEGHPSYGATVGPILLALIPLVLLRLRNASPEQSKMKTALLVLCGTA
                     FLGWLAQLAFSKLLVQTRLLFPVLPELAILATAGFDRLGSLGRRGRSARFIIGGLVGF
                     TLAITALQAALGFIAAAPLRVATGSEPDEEYLRGRLGQYAVAVQALNKLPDGSRVRFL
                     WEPRSYYCARGVTCEPDSLIDRWWHERRLGAGAEAPELLTGWRQQGVTHVLYFRLGAE
                     AVRNSGFDPLTDEDWAELERFLSDYLEFSESFGDAYLLYRIP"
     gene            complement(7171..8376)
                     /locus_tag="VI520_04420"
     CDS             complement(7171..8376)
                     /locus_tag="VI520_04420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HLF37162.1"
                     /translation="MSWLGSLGQLVLVTGVLAGLGIANYRFAVQAPGGNDFLARWVAA
                     NQWLVHGQDPYAPQVSLEAQTLIYGRPADRQAGEDVAHFAYPLFAMLFFGPFGLIPYP
                     LARAIWMTLLEVGLPVLAILSIQLSGWKPSAWIQRILILFSVLWYHGFRAVIVGQFAI
                     LEALVIVGALLAIQRHQDAVAGVLLGLSLAKPQMVVLLIPFVLLWAVSTRRLLILIWG
                     LGTIGLLLAASLALIPSWPIEWARQIIDYTTYTSRIGPPVSILAGVIPSVSRQITAFL
                     TGLGLIYLAFLWQRALGKGDRWFQWTAAMTIAVTNLIALRTATTNYVVMLPALVLVFT
                     VWNERWGKRANPIILFTLFGLFAGLWSLFLVTVEGNLEHPALYLPLPLLSLTGLWWSR
                     WWVLRELRT"
     gene            complement(8444..10054)
                     /locus_tag="VI520_04425"
     CDS             complement(8444..10054)
                     /locus_tag="VI520_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37163.1"
                     /translation="MGRVMPVSAGETGGRSAKIWVLESVALLFILAVAGYLRFTNLQS
                     NPGWYSDEGTLADIARHVNEGEVQYLAINRSTLLAARLPLAPLLASAFIPLGASPLET
                     LRVLAATSGTLTAGLVFLLTRRVTGSLGLGLLSGGLLAIYPPAVFYSRIGFSYNLLGP
                     LVLLALGGLWFYLDQGSRVGLAIAALAIGVGSLVDLMMISLAGPLTVVIFVKRRRDLA
                     IGMPLFMAPILLYAAWMLLRAPDAFWFDLRYIASRLSAIPWWAQLPLVIYNFGTLMLT
                     DPWWAPALIGLLLARPRRWRLLQLAMFLLPLILLGRSSGLAGLRLYSISPLFPFIGLG
                     TANLLWIGVPWILAIAREAIAHQLRKIRWCTIEGAGTWVNKRLTALGSAGLLFLVVLS
                     PIIVSTFELVTRVRTDFKPENGWAYLPVEQAKRVAAFVNRSMGADEFVVASPALAWAL
                     EPQTADFQQSLAYRGEPSLDYPPDVPRDRFEFEADYEQAGMAVVDSIWREWGAVHLIG
                     VSEMLRQIEMWPVLWEEGEIRVHQNPNR"
     gene            complement(10042..>10893)
                     /locus_tag="VI520_04430"
     CDS             complement(10042..>10893)
                     /locus_tag="VI520_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37164.1"
                     /translation="VLASIALITRPGNQLPWNLFAPLVAGVYLFRPTLVTFRNGQLGG
                     LLLLIIVVSMVLWRRNSWFAGGAVLALLWLKPSIALPVILLTAAWAVSARRPRAISGV
                     VVGTAALTIAGWVASASWIGDYVSIGARKLAASVGYAPSLWGLVGQACRLDSGCIFRI
                     GLITTALVLFGFALFVFKFPGPFSPSIFMACVVSVSLFVTPYVWAYDQVLLALPLAVV
                     VGEMSRSRMPFLVCASVPLLFSLGSLILLVVAFQAGSDAWSGILSLSALGSAAWIALQ
                     SRHGWVA"
BASE COUNT         2473 a         3546 c         3211 g         1663 t
ORIGIN      
        1 cagccactga tcgagtccgg cttgatcgta aacctggtcg ccgaccacga agtacccccg
       61 agcgacattg cggatcacgt ccaccagctt gccaggctca acatctttcg agcagtaggc
      121 cgcggcaccg gccttgaatg cgtgcaggac ttgctctaca tcatcatagg ccgtcaacag
      181 cacaattgcg gttttgatcc gttcggcaac cacctgccgg gtgacttgaa ttccattcat
      241 gctgggcagg tttacatcga ccaacgccac catcggctgc ttatccctca ccagctggag
      301 ggcctcatct ccactggtgg tctcgccaat aatcttgatg tcttcctctg agataagcac
      361 gttccgcacc ccctcgcgga agagcgggtg gtcgtcaacg ataagcacac tgacctcttt
      421 catggccttc cctgccacag ctcggcgagt atagcacagc caccgcacgg agcaaacgag
      481 gatggctccc agcggtacac tgccatgttc tccgacccgg ccgcgggact gaatcgggcc
      541 ccagtctcat aggccgggac cctccccgcg gtcagcttcg gataccaccc ggggaaagtc
      601 atcaggtagt ccgccccgcg ctcggatagg tagcgagcca gcgccggttc gtcccgcagg
      661 attggcacca cagttggatc gatcaatccg gccaaatcaa tcagcgtccg atgaccaaaa
      721 taaccgagcg ccccgatatc gtgggcggcg accacggcac cctcgggggt gtgggcctct
      781 acccaacggg cggtctctac catctccgat tcgatgatgg ccacatctcg gccataggcc
      841 cgcgcgccgg ccagccagaa atagccggcg atgattggca ccagcgcgat ccaggcccgg
      901 gaaaggactc gtctcccgaa gtggttcggg cgtagctcca actcgcccaa cattcccgcg
      961 ccggccagcg ccatccacac cgggagcacc gggagcgcat atcggccgtg ctgataggtc
     1021 gccggcaggc gcgccgcgta gagtccgatg tacgctgcgg accaaaccag cccgagcagc
     1081 cgatcccagc ggcgagcacg cagctgagcg atgacgaaga gcaccagccc tggaacgagt
     1141 gcggccatca ctccgatcag cgggcctccc ggatccaatc ctgtgacccc cagccacgcg
     1201 cctggaattc cggcctgggc ggccagccta atcgcgaagg ggagttcctg cagggcggca
     1261 tactcggcct gcttggcgaa ggcggtattc ggccaccacg cgccagacag cgagcgctgc
     1321 caaagcaggt agggaaccag aacggccgcg agtccggctg ccaaccgagc caggcccggg
     1381 acaatcttcc gccgctcgcc cagcagcagc cggccaagcg gcacccccac cagggtcaac
     1441 ccgtcgggtc gtaaccaaac tcccagccca atcagcatgc cgagccgacc tgggccaagc
     1501 cacccggttt cggtggccag gagaaccagc acggccagcc ccgccaccgc cagggtttcc
     1561 atccccgaca ggccggccca gaccaggtgc cactcgatgc caattagcac ggccaacgtc
     1621 catgcccagc cggcgcgctc cggcaccaga gcctgaaccc accggagggc cagccaggcc
     1681 acgagcccga gcacggccat tccgaccgcg taggtaaaca gcagaggatt gaccctgagc
     1741 caatacccga cggccaacac gcctgtccag agcggagccg tcgaaccggc actgggctcg
     1801 cccggaagga acgcccactg tttgagttcg accaggttac gggcataagt ctggtggatc
     1861 caggcatcat ctaaaggcag cccgagccga aacgtgcgct gggcggtcca caggtacagg
     1921 gccgcggaag cgagcagggc aagcgcggtt aggccggcag ccgatggccg ggcgatccgc
     1981 ggacccggcc tcacttctgg atgacctcaa gcaaatagac cggcctcccg aatgggtcgg
     2041 cgatctggtc ggccaggcgc agccacggga ccgaccctgg gcgggcataa agcggatcca
     2101 gttgggaggg gtgattggac tccaagacca cccagtccac ctgataccgg cgcacaaccg
     2161 cgtgcagggt ttccggcgga ccattcggaa tcacgaccgc ttcgcggtcg gtcgctaaac
     2221 tgaatccagg cggattgttg accgccaccc gcccctccga tcctgacaag cgctcgccta
     2281 gctccgagta agtttgcaaa cccgcgcccc aaccctggcc ggccgcggcc gggatcacca
     2341 gccggagagc caccaacccg atcgtcaacc cggccgccag caccatcgcg gccgtaccca
     2401 gcacccggcg ggcctggccg atgtcccaat tgcgcttgcg ccccgcccag tcgatcgccc
     2461 cccgcagccc gaccggggcg agcgcccaga gtaccggcat cgcggcggtg ctcgagtgaa
     2521 agaacgcccc ccgcgggcca atgaagggaa agacgatcgt catcacccca aacagcagcc
     2581 cgaggtaata gatcggcaag cggaccaacc gtctcgacca gagtcggatc gctccgagca
     2641 gcatgaaggg ccccaggaag accagcccgt tctcagcaac cagcctcgcc gaattccaca
     2701 atagggccga cccgcgagcc gcgagcaggg ttggcaggcc cgcctgcctc caccgctcga
     2761 agtccagtag ctcggccgga taactgaaca ggtcgtcata gctcaacatc cacagcaggc
     2821 gcaggctgcc cggattcgcg acgcttccaa ctgccgaaag gtttctgagc caccagggac
     2881 caagcacgag gacgaagccg accaggagcg ctccgaaccc gcgggcccgg ttctgcttgc
     2941 tgaggatcac cgcgagcacc gcgatcgcca gcagcagcag gccgtccgcc cgggcaaggc
     3001 ttccaagtcc agtcaccgca ccaaccgcca gccatcgccg ggcgcttcgt ttctccatcg
     3061 ccgacgccat gagccagaac gcgatcgcgc ccaaaaccgc ataggcggcg aagacgtcgg
     3121 tggtcacatt gaacggtaag aagaatccgg agaaggccgc caaccatccg gcctgagttg
     3181 ccagctcgcg atcctgggtg agctggaatg cgagtcggcc tgcaagcagc ggcagcacag
     3241 ccgcaatcaa cacaaacggc gcctgggccg cacggaagga ctcgccgaag agcgtcattg
     3301 aacctgccgc gaccaacgag gccagcggca gccagtacag gtgggaggga tgcggaatct
     3361 ggcttggatc gtcgaggtag ttccacagca cgggttcggt gaatccctcg ccgcgggcca
     3421 attgcctggc agtgagcatg tagtaatcgg cgtccatgta tcccggctgc cgcacccacg
     3481 ccgccgccag gagtgacatc gccaacccga tcagcagcaa ccggatcgtg tgcaatgtct
     3541 cgggctcagg tgtccgatcc gccgtgcctg ccttggaagt ggtagaggtc ggcatcaccg
     3601 aaagaggtca ccgcctcgtc tggaaagttg ttcctgagcc aggccagcac tgggtgctcc
     3661 ggaaggccca aggctgcgta gtcatcgatt tctcgacgga aaattacaaa ccaaacccca
     3721 tcgctgtcac caatcacggt cggtgcatcc gcctgggccg tcaaaccaag cacttcctga
     3781 gtggccggtg ccagcgtatc ggagcctgat ccgggtgggt cggccaggta ggcttgagtc
     3841 agctcgggcg catagtagat ggaagggaag gccgtgagct tgttgctgtg cagaacgatc
     3901 tcaccttcag ttttgttctc gccgatgtga gcaacaatct catcaaacgg cgcgtaagcg
     3961 aatccgcggt atgtccaata gctgaacagc ccaacaccga aactcagcgc tactgctgcg
     4021 actgcagtcc aacgaaatct ctgtggaagc tggtctccac ccagcaccca ggccagcccg
     4081 aacaagaacg ccactccggc aggcagcagc gcccggtcca ggtagaccgg cacccactgt
     4141 gaaacgagaa aactgagcag gaggggggcg aacgccaggt agacagacca ccccgcaatc
     4201 gcggagttgg gatcattcga ccttcttccc ttaagcaacg cccacccgcc gagtccgatc
     4261 accagaacct gggagaacag gacgatcggc agcgcccagg ttgcgatcgg caaccctcca
     4321 agatagataa tcaaagttcg aacgagctcg actgggcccg gaatcgggat ccagtaggcc
     4381 tggcgtatcc ccgcaatttg ggtagccagt cggagcagcc atggcgcgta gaggatcagc
     4441 gacccaagtc cacacaagag ggtcgcctgg actgccttcc aattccgccg atagaccgga
     4501 attagagcca tcgggagaag gtacattgcg gccagcgaat gggtgtactg ggcggccgct
     4561 gcgagaacgg cgaacccaat ccaacgcaac caaccgccct ggttgaccgc cgcccagtac
     4621 agcgcggccg cggcggttag ttcaagcgct aggagtccat acattcggat ctcctgcgca
     4681 aagtgaacct ggaagggtga cagcgccacc agccaaccga accaggccgc cagccgcggg
     4741 ccgaccatgg ctcgggcagc cgcgtaagca accccgacca gccctaatcc gacgaggacc
     4801 gagaatgccc gtaaggcaaa tggggtggta ccaaaaaccc ttccccaggc ccaaagcagc
     4861 aggtaatacc ccagggggtg gacgtcggct gtggtggctt cgacgatcgc ggccggacct
     4921 ttcacagata gcaatgcaga gaaggcctca tcgaaccaaa tgggtttggt gaccactccc
     4981 gggagccgtg ccagcaacgc cacgaacaga atcgggatcc agactcgccg cccgatttgc
     5041 aaccctcgcg gctcttgctt aggcgcagtc tggatcgcgc gcttcgattc gagcatcaag
     5101 gtatccgata cagcaaatag gcatcgccga atgattcgct aaactccaga tagtccgaca
     5161 aaaagcgctc gagttcagcc cagtcctcat cggttagtgg atcaaaacca gagttgcgaa
     5221 ccgcctctgc acccaggcga aagtacagga catgggttac accttgctgg cgccaacctg
     5281 tgaggagttc tggggcctca gctccagccc caagccgacg ttcatgccac cagcggtcga
     5341 tcagcgaatc cggctcacag gtaaccccac gggcgcaata atagcttcgt ggctcccaca
     5401 ggaatcggac ccggctgccg tctggtaact tattgagggc ctggacggcc acggcatact
     5461 ggcccagccg gccccttagg tattcttcat ccggctctga tccggtggct actcggagcg
     5521 gagcggcggc aatgaatcca agggcggcct gcagcgcggt aatagccaaa gtgaagccga
     5581 ccaagcctcc gatgatgaat cgggctgaac gcccgcgacg cccaagcgac ccaagccgat
     5641 caaaaccggc cgtggccagg attgccagtt ccgggagcac cgggaacagc aatctggtct
     5701 gcacgagcag tttcgagaat gccagctgcg ccagccagcc gagaaaggcg gtcccgcaca
     5761 agactaggag tgcggtcttc atcttgctct gctcgggcga agcattcctt agccgcagca
     5821 gcaccagcgg gatgagtgcg agcagtatcg gtccgaccgt cgcaccgtag ctaggatgtc
     5881 cttcgaaata gcctccttcc aggccccaaa tcgtcgcctc ccatggcgcg actaagagct
     5941 ggccggctcc aagtccggtt cctgcccggc tgaaccaggc agcccgatag gcatcccaga
     6001 acctgccaga aaagaaaaac gggtatacgg ggttcgaggt caggagccag ttcttcatga
     6061 agaacgggat caagaaagtt gaaaccgatc cggcaaagag caccacttcg ccagtcgacc
     6121 ggcgccgcag gtacgcaacc aatccgaggc cggcgaccat ccatactgca gagtacttcg
     6181 tgttcagcgc gagggcggca aataggccag ccaggacgat ccgcaggatc cgttgggatc
     6241 cgatcgacct cgccagctcg ccggtgccga cggattcacc agtcggaccg acatcatccc
     6301 cggttgtcag cagcacgaaa cacgccaggc ccgcgaaggc cgtgatccat tcgacatagg
     6361 ccaggccgag cagcagttgg gcgctgggta ctccgagcaa gatggccgcg gcgagccagc
     6421 cacgccccgg agcaactcgc cgcaccacag ttgggagcaa ggcgagggtg agtagaccga
     6481 acgaaaggtg aatcaactgg ggaacccgat ctcccgccag caacatgccc cagaggaaaa
     6541 ccatcgagcc tgcctgcgga aacccgagat aaggtagatc caagtcgtgg gtcagcctgc
     6601 ctgcctcgag atatagcttc ggtccggtga gatggtagac cagcgagtcc catgcggttg
     6661 gaggtgccag cgccgatacc gcagccaacg aaatcagccc gagcacaaat gcagccagcc
     6721 atcggttggc tcgtccgcta ggcaagccga ttcggcgttc tcgaagcatc ccgatccatt
     6781 ggcggagcga gaacggcagc gagagagtcg cgatccccca ggctactgtg gggtggtatg
     6841 ccccaagcgc ccccatactg agcgccaaga agctcagacc ggccatgccg gcgccgagct
     6901 gcaaggcgat ctgagacctg gcttccaacc catctagtgc acgcctccat gggagtccca
     6961 acacggtcgc cacactcagg gtcatgagca ccccggcaac cgatcccgtt gccctggcga
     7021 gcgccccgag ctgcgctggg ctgcccggct tgtgaacggc aaagtaagcg ctagtcacga
     7081 taacaaccca agtcactgca acgattgcta gcagaaaccg cttacggcgc tcctgtgtgt
     7141 cttccttatg gatcatgtgc ggtcacggcc tcaagttctc agctcccgca gcacccacca
     7201 cctcgaccac cacagcccgg tgagcgagag gagcgggagt ggtagataga gggctgggtg
     7261 ctctaagttt ccctcgaccg tgaccaggaa caagctccac aatcctgcga atagtccgaa
     7321 caaggtgaag aggattattg ggttcgctcg tttcccccac cgttcattcc agacagtgaa
     7381 caccaggaca agggccggaa gcataacgac gtagttggtg gtcgcggtgc gaagcgcaat
     7441 cagattcgtg acagcaatcg tcatcgccgc ggtccactgg aaccatcgat cccctttgcc
     7501 caaggctcgc tgccagagga atgccaaata gatcaaccca agcccggtca gaaatgccgt
     7561 gatctgtctg gagacgctcg ggatcactcc ggccaggatg gacaccggcg gccctatgcg
     7621 ggaggtataa gtggtgtagt cgatgatctg tctcgcccac tcgattggcc aacttgggat
     7681 caatgccagg gacgctgcga gcaggagccc gatcgtacca agtccccaga tcaagatcag
     7741 gagtcgacgg gtcgataccg cccagagcaa gacgaacggg atgagcaaga cgaccatttg
     7801 aggcttggca agcgacaatc ccaacaagac ccccgcaacc gcatcttggt ggcgttggat
     7861 ggccaagagc gccccgacaa tgactaaggc ttcgaggatg gcaaactgcc cgacaatcac
     7921 ggcccgaaac ccgtgatacc aaaggaccga gaataggatc agaatccgct ggatccaggc
     7981 actcggcttc cagccgctta gctgaatgct caggatagct agaactggca gcccaacttc
     8041 aaggagtgtc atccaaatcg cgcgggcaag cggatacggg atcaggccga atggcccgaa
     8101 gaaaagcatg gcgaacagtg gatatgcgaa atgggcgaca tcctccccgg cctggcggtc
     8161 tgctggtctc ccatagatca gggtctgtgc ttcgaggcta acttgggggg catagggatc
     8221 ctggccgtgt accagccatt ggttggccgc cacccagcgc gccaggaagt cattgccgcc
     8281 cggagcttgg accgcgaacc ggtaattggc gatgcccaat ccagccagca cgccggtcac
     8341 caacaccagc tgcccaaggc ttcccaacca actcaagctc gaccgcctca agcttccctg
     8401 tgccttcctg ctcgaactgc gcctgatcgc cggatgacca ggttcagcga ttcgggtttt
     8461 gatgcactcg aatctcgccc tcttcccaga gaactggcca catctcgatc tgccgaagca
     8521 tctcactcac gccaatcaag tggaccgccc cccattcacg ccaaatcgaa tcaaccacgg
     8581 ccatcccagc ttgctcgtaa tccgcctcga actcaaatcg atcccgaggc acgtccggtg
     8641 ggtagtccag agaaggctcg ccgcggtagg ccagcgactg ctggaaatcg gctgtttggg
     8701 gctccaaggc ccaggccagt gccggggatg ccacgacaaa ctcatcggcg cccatggaac
     8761 ggttcacgaa ggcggccacc cgcttggcct gctcaactgg caggtatgcc cagccgtttt
     8821 cgggtttgaa atccgttcga acccgcgtga ccagctcgaa ggttgaaact atgattggac
     8881 tgaggacaac cagaaacagc agtccggccg atccaagtgc ggtcagccgc ttgttaaccc
     8941 aggtgccggc gccctcgatg gtgcaccagc gaatcttgcg cagctgatgg gcgatggcct
     9001 ctctggcgat tgccaggatc caggggacgc cgatccagag caaatttgca gtccccaagc
     9061 caataaatgg aaagagaggg ctgatcgagt agaggcgcag cccggccagg cccgagctcc
     9121 gtccgagcag gatcagcggg agcagaaaca tcgccagttg gagcagccgc cacctccgcg
     9181 gccgggccag gagcaatcca atcagcgcag gagcccacca cggatccgtg agcatcagag
     9241 ttccgaagtt gtaaatgacg agcggcagtt gagcccacca cgggatcgca cttaggcgac
     9301 ttgcaatgta tcgaagatcg aaccagaagg cgtcgggggc gcgcagcagc atccaggccg
     9361 cgtacagcag aattggcgcc atgaagagag gcattccgat tgccagatcg cgccttcgct
     9421 tgacgaaaat gacgacagtc agcggaccag ccaggctgat catcatcaga tcgaccaatg
     9481 agccgacacc gatcgccaga gcggcgatcg ctaggcccac tcggctcccc tggtcgaggt
     9541 agaaccatag tcccccgagc gcgagcaata ccagcggccc aagcaggttg tagctgaatc
     9601 cgatccggct atagaaaacc gcggggggat agattgccag tagcccaccg ctcagcaatc
     9661 cgaggccgag cgagccggtg actcggcgcg tgaggaggaa taccaggccg gccgtcaggg
     9721 tgccggaggt cgcggccaag accctcaggg tctcgagcgg gctggccccc agcggaatga
     9781 aggccgaagc cagcagcggc gccagcggca agcgagccgc caacagcgtc gaccggttga
     9841 ttgccaggta ctgaacctcg ccctcattca cgtgccgggc gatatccgcc agcgttcctt
     9901 cgtcgctgta ccagccggga ttggactgca ggtttgtgaa acggaggtag cccgcgaccg
     9961 ccaggatgaa caaaagggcg accgattcca aaacccagat ctttgccgac cgtcctccgg
    10021 tctccccggc gctcacgggc attacgcgac ccacccatgc ctgctttgta gcgcaatcca
    10081 agctgcggat cccaaggcgc tcagactcag gattccgctc cacgcgtcgc tccctgcttg
    10141 gaatgccaca accagcaaga tcaatgagcc gagactgaac aacagaggaa cacttgcaca
    10201 gaccaagaag ggcattctcg atctgctcat ctctccaacg accaccgcca aaggaagcgc
    10261 cagcagcact tggtcgtatg cccagacata cggtgttacg aacaaggaga ccgacacgac
    10321 gcaggccatg aagatgctgg gggagaacgg gccaggaaac ttgaacacga agagggcgaa
    10381 cccgaagagg actagggcgg tggtgatcaa gccgatccgg aatatgcaac ccgagtctag
    10441 tcggcatgct tggccgacga gcccccagag actgggcgcg taacccaccg aggcggccag
    10501 ctttcgcgcc ccgatactga cgtaatctcc aatccaggat gcgcttgcaa cccacccggc
    10561 gattgtgagc gcagcagtgc ccaccacaac acccgagatg gcacgcggcc tccgagctga
    10621 gacggcccag gcggcagtaa ggaggataac cggcagggct atgcttggct tgagccacag
    10681 cagcgccaat acggcgccgc ctgcaaacca gctatttctc ctccagagaa ccatgcttac
    10741 tacgatgatg agcagcaaca gtccgcccaa ctgcccatta cgaaatgtga ctagcgtcgg
    10801 gcgaaagagg tagacccccg caacaagagg agcgaatagg ttccagggca gctgattgcc
    10861 tggccgggtg atcagcgcga tcgaggcgag aac
//
LOCUS       DATDPQ010000188         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2700134, whole genome shotgun sequence.
ACCESSION   DATDPQ010000188 DATDPQ010000000
VERSION     DATDPQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700134"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VI520_04435"
     CDS             <1..1187
                     /locus_tag="VI520_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37165.1"
                     /translation="RRGNPDPDRTPALPWPVALDTEALRRSLFLAGVVALSVASGLGF
                     FVRALGGVFGGLGGWAEGWFEPSGIPAGSVMLNLPTYEPLLLGFGGLGLVRAVRKGSA
                     LESWLAVWAISALLLIAVYPGRRPEDLLWAVIPLCFLAAKEASQLIERALGARPAWLL
                     VGFVAAVIALLAFSYMQLAAFVRGPDAFQTLLGIPLLLMLALMGIGLAAAAIGLLGLG
                     WSREIAGTVAGVAGLIVLSSLSLSGGGRLNLAPASASELWRPQASLAGLLDLRETIDT
                     LSKAETGRTAAIPLQFADPSTPALAWVLRGYAPRGAADPADNPPVILMQEGSGAGLFP
                     SEYLGQSVSIGERWGWTGWWPPEFLSWWVTREAPTLPDRWVLLVRPDIAGVEQPISTG
                     EP"
     gene            1184..1870
                     /gene="cmk"
                     /locus_tag="VI520_04440"
     CDS             1184..1870
                     /gene="cmk"
                     /locus_tag="VI520_04440"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HLF37166.1"
                     /translation="MIPSTIAIDGPAAAGKSTLARSLAAQLGYLYFDTGVMYRAVTLA
                     AQRAAIAVDDEPQVSAIAEQVAIDVQPASVPDGRQYDVLLDGEDVTWAIRSPEIDANV
                     STVSMYGRVRRAMTERQREIGRRGRVVMVGRDIGTVVLPEAELKIFLKADVEDRAQRR
                     YDEMRARGEAAEYAAVLDAVQTRDQLDSTRDLAPLRPAADAIIVDSTDLDPQQVLERV
                     LELVAELHRG"
     gene            1907..>2521
                     /locus_tag="VI520_04445"
     CDS             1907..>2521
                     /locus_tag="VI520_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLF37167.1"
                     /translation="MTQKEYQHARFEPRRRVLRWLIDNVGYRVFAKFNGIENVEHLPL
                     SGPAIIMMNHIAMIDPIVVLGGLPRNIVPLARHDVFRLPLWGIFPRLWNVIPVRRGEV
                     DRSAVQRALAVLKAGEVVLVAPEGTRNTQLQQGKVGVAYLGARSGAPIVPAAVEGTDG
                     YPSLRLDRSQEPGATVRLGRPFRFKVSSGRLDRELLRRMTDEAMY"
BASE COUNT          388 a          771 c          875 g          487 t
ORIGIN      
        1 ggcggcgcgg aaatccagat ccggatcgaa ctccggcatt gccgtggccg gtcgccctgg
       61 ataccgaagc gctgcggcgc agtctattcc tggctggcgt ggtggctcta agcgtcgcaa
      121 gcggcctcgg attcttcgtg agggcgctcg ggggagtctt cggagggctg ggtggttggg
      181 ccgagggctg gttcgagcca agtggcatcc cggccgggtc ggtcatgctg aatttgccga
      241 cctatgagcc gctgctgctt ggtttcggcg gcctgggttt ggtgcgggcc gttcgcaaag
      301 gttccgccct cgaatcctgg ctcgcggtct gggcgatcag cgcgctactg ctgatcgccg
      361 tctatcccgg ccgccggccg gaagacctgc tctgggctgt cattcccctg tgcttcttgg
      421 ccgcgaagga ggcctcccaa ctgatcgagc gggccctggg ggcccgccca gcctggctgc
      481 tggtcggttt cgtcgccgcc gtgattgcct tgctggcgtt ttcctatatg cagctggcgg
      541 ccttcgtgcg cggtcccgac gcattccaga ctctcttggg cattccgctg ttgttgatgt
      601 tggcactgat ggggatcggg ttggcggccg cggccatcgg gctgctcgga ctcggctggt
      661 cgagagagat cgcaggcaca gttgccggcg tggccgggct gatcgtcctg agctcgctca
      721 gcctctccgg cggcgggagg ctgaacctgg caccggcctc ggccagcgag ctgtggagac
      781 cccaagccag cttggcaggt ctgctcgacc tgcgcgagac gattgacacc ctctccaagg
      841 ctgagaccgg ccgcaccgcg gccattccgc ttcaattcgc cgatccgagc accccggcgc
      901 tggcctgggt gctgcgcggc tacgccccga gaggtgccgc cgatccggcg gacaatccgc
      961 cggtgatcct catgcaggaa ggatcgggcg ccggattgtt tcccagcgaa tacctcggac
     1021 agtcggttag tattggcgag cgctggggct ggaccgggtg gtggccgccg gagttccttt
     1081 cctggtgggt aacccgggag gcgccgaccc tgccggaccg ctgggtcctc ctggttcgac
     1141 cggacattgc gggggtggag caaccaatct caactgggga accatgatcc cgagcacgat
     1201 cgccatcgat ggcccggccg ccgcggggaa atcgaccctg gccagatcgc tggccgccca
     1261 gcttggctat ttgtacttcg ataccggggt catgtatcgg gcagtcacct tggctgcgca
     1321 gcgggcggca attgctgtcg acgatgaacc gcaagtttcg gcgatcgccg agcaggtcgc
     1381 gatcgatgtc caaccggcct cggtcccgga tggacgccag tacgacgtct tgttggatgg
     1441 ggaggacgtg acctgggcga tccgctcgcc cgagatcgac gcgaacgtat ccacggtgtc
     1501 gatgtacggc cgggtgcgcc gggcgatgac cgagcggcag cgggagatcg gccgccgagg
     1561 ccgggtcgtg atggtcggcc gcgatatcgg aaccgtggtg ctgcccgaag ccgagctcaa
     1621 gatctttctg aaggctgatg tggaagaccg cgctcagaga cgctacgatg aaatgcgggc
     1681 tcgtggcgag gccgcagaat acgccgcggt tctggatgcg gttcagactc gcgaccagct
     1741 tgactcgact cgcgatctgg ctcccctgcg gccggccgcg gatgcaatca ttgtcgactc
     1801 aaccgatctg gacccccagc aggtgttgga gcgggtgctc gagctggtgg ccgaactcca
     1861 ccggggctga tccgcccctg aagttgccgc cgcaccgatc tatcgaatga cccagaaaga
     1921 atatcagcat gccagattcg agccgcgccg ccgggtgctt cgctggttga tcgataacgt
     1981 cggctatcgg gtgtttgcca aattcaacgg gatcgagaat gttgagcacc tgcctctctc
     2041 gggcccggcg atcatcatga tgaatcacat cgcgatgatc gatccgattg tcgtgctcgg
     2101 gggcctgccg cgcaacatcg ttcccctggc acgccatgac gtgtttaggc tgcccttatg
     2161 ggggatattc ccccgactct ggaatgtcat ccctgtgcgc cgcggggaag tggaccgcag
     2221 cgcggtccaa cgtgcattgg cggtcctcaa ggcgggtgaa gtggtgctgg tcgcaccgga
     2281 gggtactcgc aatacccagc ttcagcaggg aaaggtgggg gtggcctatc tgggggcccg
     2341 cagcggcgcc ccgatcgtgc cggccgcggt cgaagggacc gatggctacc cttcgctccg
     2401 gctggaccgc agccaggagc ctggagccac ggtgcgactt ggccggccgt ttcgattcaa
     2461 ggtaagcagt gggcgtttgg atcgggaatt gctgcgcaga atgaccgatg aggccatgta
     2521 t
//
LOCUS       DATDPQ010000189         3166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_597925, whole genome shotgun sequence.
ACCESSION   DATDPQ010000189 DATDPQ010000000
VERSION     DATDPQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597925"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VI520_04450"
     CDS             complement(<1..1479)
                     /locus_tag="VI520_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37168.1"
                     /translation="MLKGLSKLLGGDSLARDLERYETLARRINSLEPTMRALADPELR
                     AKTAEFKTRLASGESVDALLPETFAVVREVSVRTIGLRHFDVQLIGGAALHEGRIAEM
                     KTGEGKTLVATLPLYLNALTGRGVHLVTVNDYLARRDARWMGPLFSFLGMEVGVLQEA
                     ARTEHGRKAFTFDPSRTTGQEDVFQLQLVDRRVAYQADVTYGTNNEFGFDYLRDNMAP
                     TLEARSQRGHHYAIVDEVDNILIDEARTPLIISGPAEEDPALYQQMTGVVRQLEPEDY
                     EISERDRTISLTEIGEDHVERLLGQPLRDPDRPEDITPEQARLLGYLEQSLRANFIFK
                     RNKDYVVQGGRVIIVDEFTGRMMPGRRWSDGLHQAVEAKEGVRIREENVTYATITLQN
                     YFRMYDKLAGMTGTALTEAEEFNKIYSVDVLPLPTNLEYIARQPDSELVELQVQENGR
                     RFGYFARRGDPEHTPVFWRRKDLPDLVYRTEDAKLRSVSLEIL"
     gene            1587..1670
                     /locus_tag="VI520_04455"
     tRNA            1587..1670
                     /locus_tag="VI520_04455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1621..1623,aa:Leu,seq:taa)
     gene            1727..2629
                     /locus_tag="VI520_04460"
     CDS             1727..2629
                     /locus_tag="VI520_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HLF37169.1"
                     /translation="MPHRWLPLLVLAGTLLSCTLPGANSPTPFAFPTPDLTLTAAFAP
                     SPTPLIPTRTLPPANTPTPTATQVIPGATAGATGTSSPATLDTRPNGSPVEAALLETP
                     PTIDGDLADWADPGRELANVVFGASNWTGAGDASARFHIGWTEQGLYLGLQVTDDKRV
                     QVSSGRNIFKGDIVELQLDSDLTGDFFSNSLTSDDYQIGLSPGNFGSLASEAWRWYPI
                     GLAGSLSGVSLRAVTTTEGYNLEAMIPWTVFGVTPDAGDRFGFALSISDNDLTGAPVQ
                     QSMVSTISTRTLTNPTTWGTLILK"
     gene            complement(2707..>3166)
                     /locus_tag="VI520_04465"
     CDS             complement(2707..>3166)
                     /locus_tag="VI520_04465"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37170.1"
                     /translation="LATRHYPEAEAAYREVFEQDETNSEAALGLVESLLIQGKGSEAL
                     SILQNFPPGTAWVKAEGYLPLASLLNEVETAAPNLDLPPLDASLFQAGRLIGRGNLPA
                     AMDGLLDVLRQEKTYRAGLPRKVLLALFSLLGDLDPLTRKYRDELASVLF"
BASE COUNT          571 a         1016 c          937 g          642 t
ORIGIN      
        1 gaggatctcc aggctgaccg aacgcagctt ggcgtcttcg gtccggtaga cgagatccgg
       61 caaatccttg cgtcgccaga aaaccggcgt gtgctccggg tctccccggc gggcgaagta
      121 accgaaccgg cggccgttct cctggacctg aagctcgacc agctcggagt ccggctgtct
      181 tgcgatgtac tccaggttcg tcggcagcgg cagcacgtcc acgctgtaaa tcttgttgaa
      241 ttcctccgct tcggtgagtg cagtcccagt catgccggcc aacttgtcat acatccggaa
      301 gtaattctgg agggtgatcg tggcgtaggt gacattctcc tcccggatcc gaaccccctc
      361 cttggcctcc accgcttgat gcaggccgtc ggaccaccgc cggcctggca tcattcgacc
      421 ggtgaactca tccacaatga tgacccgacc gccctggacc acgtagtctt tgttccgctt
      481 gaagataaag ttggcccgca ggctctgctc caggtagccg agcagccggg cctgttcggg
      541 agtgatgtct tccggtcgat ccggatcccg caatggctga cccagcaacc gttccacatg
      601 gtcttcgccg atttcggtga gcgagatggt gcgatcccgc tcgctgattt cgtagtcttc
      661 tggctccagc tgtctcacca ccccggtcat ctgctggtag agggcggggt cttcctctgc
      721 aggtccggag atgatcaacg gggtccgggc ctcgtcgatc aggatgttgt cgacttcgtc
      781 tacgatagcg tagtgatggc cgcgctggga gcgggcttcc agcgtcgggg ccatgttgtc
      841 ccgcaagtag tcgaatccga actcgttatt ggtgccgtag gtgacgtcgg cctggtaagc
      901 cacccggcgg tccaccaact ggagctgaaa tacatcctcc tgcccggtcg tccggctcgg
      961 gtcaaacgta aaggcctttc gaccgtgttc ggtccgagcc gcctcttgca acactcccac
     1021 ttccatcccc aggaagctga agagtggtcc catccagcgg gcatcccggc gggcgaggta
     1081 gtcgttgacc gtaaccaagt gaaccccgcg gccggtcagc gcgttgaggt agagcgggag
     1141 agtggcgacc agggtcttgc cttccccggt tttcatttcg gcgattcgcc cttcgtggag
     1201 tgccgcgccc ccgatcagtt gcacatcgaa gtgtcgcaac ccgatggtcc gaacggatac
     1261 ttcccggacg acggcaaatg tttctggaag caatgcgtcc acggattcgc cggaggccag
     1321 ccgggtcttg aattcagcgg tcttggcgcg cagctccgga tcggccaggg cgcgcattgt
     1381 gggctcgagc gagttgatcc ggcgggccaa cgtctcatag cgctctaagt ctcgagccag
     1441 tgaatctccg ccaagcagct tcgatagacc ctttaacatg gaaatcggcg ggattatagc
     1501 atcctggtcg cgcgggccgc acttcccggg atgagccgca cctccagtaa ctcaccggag
     1561 agtggcgacc taggctagaa tcaggtgggg cggtggcgga actggtagac gccgaggact
     1621 taaaatcctc tgagggtgac ctcgtgtggg ttcgagtccc acccgcccca ctcagcggcc
     1681 acgcagattt ccccaaatcc acgatgggtg tacaatcgcc cgccgcatgc cccaccgatg
     1741 gttgcctctg cttgtcctgg ccggcacgct gctctcttgc accctgcccg gcgcgaactc
     1801 tcccacccca ttcgccttcc cgactcccga cctcactctg actgcggcgt ttgccccctc
     1861 cccgactccg ctgattccga cccgaacctt gccgccagcc aatactccaa cccccaccgc
     1921 cactcaggtc attcccggcg cgaccgccgg cgcgaccggt acctcctccc ccgctaccct
     1981 tgacacccgc ccgaatggct ccccggtcga agccgctttg ctcgagaccc caccgacgat
     2041 cgatggcgat ctcgccgact gggccgaccc gggccgcgaa ctggcgaatg tcgtgtttgg
     2101 agcaagcaac tggaccggcg ccggggatgc ctcagccaga ttccacattg gttggaccga
     2161 gcaggggctg tatcttgggt tgcaagttac ggacgacaag cgggtgcagg tatccagtgg
     2221 acgaaacata ttcaagggcg acatcgtcga gctccaactc gacagcgatc tgactggtga
     2281 cttcttctcg aattcgctga cgagcgacga ttaccaaata ggactatcgc cagggaattt
     2341 tggttcgctg gcgagtgagg cctggcggtg gtatcccatt ggcctggccg ggtcgctgtc
     2401 gggagtgagc ctgagggcgg tgaccactac ggagggttac aacctagagg cgatgatccc
     2461 ctggacggtg tttggggtga cgcccgatgc gggcgaccgc tttggtttcg cactctccat
     2521 ttcggacaat gacctgactg gggcacccgt tcagcagtca atggtatcca cgataagcac
     2581 ccgcaccctg accaatccca caacttgggg cacccttatt ttgaaatagg ctcggcccgg
     2641 gcttgatgat tcgcaacaaa aaaggggccg ggcgaattgc ccggcccctt agcgtcctcg
     2701 gcccgactag aacagcacgg aggccagttc gtctctgtac ttgcgggtca gtggatccag
     2761 gtcacccagc agggagaaca gcgccagcag tactttcctg ggcaatcccg cccgataggt
     2821 tttctcctgc cggaggacgt ccagcaaccc gtccatcgcc gccggaagat tcccccgccc
     2881 gatcagcctg cccgcttgaa acaggctggc gtcgagcggc ggcagatcga gatttggcgc
     2941 agccgtttca acttcgttga gcaacgaggc taggggcagg tatccctctg ccttaaccca
     3001 ggcagtacct ggagggaagt tctgcagaat gctgagcgct tccgaccctt tcccctgaat
     3061 gagcagcgat tcgaccagtc ccagggccgc ctcggagttc gtttcatctt gctcgaagac
     3121 ttctcgatag gccgcctcgg cttctgggta gtgacgggtg gcgagc
//
LOCUS       DATDPQ010000190         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2427481, whole genome shotgun sequence.
ACCESSION   DATDPQ010000190 DATDPQ010000000
VERSION     DATDPQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427481"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VI520_04470"
     CDS             complement(<1..1071)
                     /locus_tag="VI520_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HLF37171.1"
                     /translation="MATDAARLTPIMETTRPQKVLLGGITALAQSAWMGFALAANVLE
                     QLRLAPAGILQRLTVVRPGLPRRIRLKGLLTREFSIGEASFILMASFFLSAALGAARQ
                     VLFNAQFGVSPEANAYYAAFRLPDTLFSLIAGGALSSAMIPVLLSAKQQEGEAAGWRL
                     ISSVLTTLLGTFVVLILAVEVFAPVLVERVLAPGFDAATSALTVNLTRIMLLQPLVLL
                     LGSVATAVLNSRNQFLLTGLSVVSHNISLILSILAVGQFPSLGILGPTLGVIGGAFLQ
                     VLILSPGLRGQGYRVRLMWELGNRRLREVLRLLGPNGLSVGVNYSGFIVDTAFASAAL
                     DPAGLPAIYNAFLLVGLPIALLG"
     gene            1280..1888
                     /locus_tag="VI520_04475"
     CDS             1280..1888
                     /locus_tag="VI520_04475"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLF37172.1"
                     /translation="MRITLVGAGNVGGSLGRAWAAAGHQVIFGVRDPAAKKAALKLAT
                     PYLMAAVDTVQGAIAQGEIIVFAVPATAVEPIVQANASELNGKILIDATNDFGPGLMS
                     SIATLVHHAPNAKVFRAFNSLGWENFAHPNFDGEAADLFYCGSSDAAAQQTVESLIQQ
                     VGLRPIRVGDLSADPLLDAVLRIWVVLSGAKGRHLAFKVLHD"
     gene            complement(1964..>2415)
                     /locus_tag="VI520_04480"
     CDS             complement(1964..>2415)
                     /locus_tag="VI520_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HLF37173.1"
                     /translation="VYDLSSFRQLATFSYPGEGWGLTHDGLRLILSDGTPTLRFLDPE
                     SLAEIGQLSVTDGRQPVGGLNELEFVEGEVFANLWPTDRVARISLETGQVVGWIDFGG
                     LLPAASRTPGVEVLNGIAYDAERGRLFVTGKRWPSLFEIELVLVEEG"
BASE COUNT          468 a          782 c          781 g          384 t
ORIGIN      
        1 gcccagcagt gcgatcggca gcccgaccag cagaaacgcg ttatagatcg ccggcagtcc
       61 cgctggatcg agggcggccg aggcgaaggc ggtgtccaca atgaagccgg aataattgac
      121 gccgaccgag agcccgttcg gcccgagcag ccggagcacc tcccgcaacc gccggttgcc
      181 cagctcccac atcagacgca cccggtaacc ctgcccgcgc aaacccgggg acaagatgag
      241 cacctgcaga aacgcgcctc cgatcactcc cagcgtgggc cccagaatgc ccagactggg
      301 aaactgaccg acggccagga tcgagaggat caggctaatg ttgtggctga caaccgacag
      361 tcccgtcagc aggaactggt tgcggctgtt caacaccgcc gtggccacgc tccccagcag
      421 caggaccagc ggctgcagca gcatgatccg ggtcaagttg acggtcaggg ccgaggtcgc
      481 cgcatcaaag cccggggcaa ggacgcgttc taccaggacc ggagcgaaaa cttctacggc
      541 caggatcagc acgacgaaag tgccaagcag ggtcgtgagc acgctgctga tgagccgcca
      601 tccggcggcc tcgccctcct gctgcttggc actcagcagc accgggatca tggcgctcga
      661 cagcgcgccg cctgcgatga ggctgaagag cgtatctggc aggcggaagg cagcgtagta
      721 ggcgttggcc tccggactga cgccgaactg cgcgttgaag agcacctgcc gcgccgcgcc
      781 cagcgccgcg gacagaaaga aggaagccat caggatgaag ctggcctcgc cgatgctgaa
      841 ttcgcgggtc agcaggccct tgagccgaat ccggcgggga aggcccggcc ggaccaccgt
      901 cagccgctgc aggatgccgg cgggcgccaa ccgcagctgt tccaacacat tggcagccag
      961 cgcaaagccc atccaggcgc tttgggccag ggcggtgatc ccgccgagca gcactttctg
     1021 tgggcgggtg gtctccatta tgggggtcag ccgagcagcg tcagttgcca ttgtcgttgt
     1081 ctccggtgag gcgggttcgc ggtaagagta cgcctgggcc gccaggctgc atgtcgtccg
     1141 ccgccaatta tgacattgga cagttcaagg aggttatccg cgctgctcgg ctggcagagc
     1201 tcaccagctc gcactcgctg gcggtatcat tcaggcgggt cgccacgccg gcccatttcc
     1261 ccgaccgggg agggaacgga tgagaatcac cttggtgggt gccgggaacg ttggagggtc
     1321 actgggtcgg gcctgggcag ccgccgggca tcaggtgatc ttcggagtcc gcgatcctgc
     1381 ggccaagaag gcagcgctca aattggccac gccgtacctc atggcggcgg ttgatacggt
     1441 gcagggggct atcgcccagg gtgaaatcat cgtgttcgcg gtcccggcga cggcggtcga
     1501 gccgattgtt caggcgaacg cctccgagct caacggcaag atcctgatcg acgccactaa
     1561 cgacttcggc ccgggtctga tgagcagcat cgcgaccttg gtccatcacg cgccgaacgc
     1621 caaggtgttt cgagccttca actccttggg ctgggagaac ttcgcccacc cgaactttga
     1681 cggcgaagcg gcagatcttt tctactgcgg ttcctcggat gcagccgccc agcagacggt
     1741 cgagagccta atccaacagg tcggtctgcg gccgatccga gtgggtgacc tgagcgccga
     1801 tccactgctc gatgccgtcc tcaggatctg ggtcgtgtta tccggagcaa agggtcgtca
     1861 cctggcgttc aaggtgttgc acgactagct gaatcgaggc tctctcgagg gcttccccag
     1921 gcggcccacc ggcggcccac cggcggcaca ccacgtgggc cggctagcct tcctctacca
     1981 agaccagttc gatctcaaaa aggctcggcc accgtttccc ggtcacgaac agtcgccctc
     2041 gctcggcgtc gtaggcgatg ccattgagta cttcgacgcc gggtgtgcgg ctcgcggctg
     2101 gcaagaggcc cccgaagtcg atccaaccca cgacctgccc cgtctcgagc gagatgcggg
     2161 ccactcgatc ggtcggccac aggttggcaa agacctcccc ctcaacaaac tccaactcgt
     2221 tgagcccacc gactggttga cgcccatcgg tcacagaaag ctgaccgatc tcggccaacg
     2281 actccggatc gaggaagcgc agggttggag tgccgtcgct caggatcagg cggaggccgt
     2341 cgtgggtcag gccccagccc tcgcccgggt aagagaacgt ggccaattgg cgaaagctgc
     2401 tcaagtcgta gacgt
//
LOCUS       DATDPQ010000191         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2957616, whole genome shotgun sequence.
ACCESSION   DATDPQ010000191 DATDPQ010000000
VERSION     DATDPQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2957616"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VI520_04485"
     CDS             complement(<1..315)
                     /locus_tag="VI520_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HLF37174.1"
                     /translation="MLRWFFTVVFRLLSRSRIEGLEHLPRSCPYILVGNHLSVVDAPL
                     AYLYVGGPHITGFVASKWRYHPLYFPIIRLAGGIFIRRGEVDREALHAAIDWLRAGKC
                     FGM"
     gene            247..408
                     /locus_tag="VI520_04490"
     CDS             247..408
                     /locus_tag="VI520_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37175.1"
                     /translation="MLKTLDPGAAQESEDYCEKPAQQAMAHKTLDCRRKTGVLQSAPS
                     SVIVAVLLI"
     gene            481..1947
                     /locus_tag="VI520_04495"
     CDS             481..1947
                     /locus_tag="VI520_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37176.1"
                     /translation="MMPLAPPRPVHPWLKKLLVAFVPGETSSLMEQVVDGLLEQFQSL
                     GHQVRTSPEGRTDLVLTTARFGEPLPWRKALFFTARKRFGLENNPAIYTLVQVGTREY
                     QAALDHFQAALEREPPNPADFEFPGLAPDSNRVLLEQSRRGGPILALERQVQAQAKSI
                     RVLLIIGDDRPLWAHHFDLVGAHPRIDMVEGNGFYQDLALRMVTVLSTEEVTDHEVVA
                     GLVPQAEWAALEAPKGMLLAGSELGRRGFFTEMVRISDLVAVPAVSEGVANQYSEGCF
                     ATWDPKLGALIATVTGSARPVDKDAIGENDLAVIVGVRPDGKGALVRHVAGKRNDPPS
                     SEAVEMMEMDSLLPKIRLDKKWETQATVPVVRSKLHGHRGIAAYDPARVEHVALEPTY
                     YHYPVSCATQAQAWAIKDAFARSAALQDTADPRQLVFTVLPGHGVVIAEKWLPGKAPF
                     QAIWEAIDAGELEISNKVPQGLLRYVPGPNGRMILKEG"
     gene            complement(1971..>2236)
                     /locus_tag="VI520_04500"
     CDS             complement(1971..>2236)
                     /locus_tag="VI520_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37177.1"
                     /translation="NGVGVPGVLGALSTTLSCVVAPARLAAALLGLVAWFVAAKEALD
                     LDWVQVVVTVVLAFIAGLAITFVAGLVFAALGLGTATVGGLFS"
BASE COUNT          430 a          691 c          727 g          388 t
ORIGIN      
        1 catcccaaag cacttgccgg ctcgcagcca atcgatcgcc gcgtgcaggg cctcgcggtc
       61 gacctcgccg cggcggatga agatcccgcc ggccaagcgg atgatcggaa agtagagcgg
      121 gtggtatcgc catttggagg cgacaaagcc ggtgatgtgc ggccccccca catagaggta
      181 agccaacggt gcgtccacca cgctcaagtg gttgccgacc aggatgtatg ggcagctccg
      241 aggaaggtgc tcaagaccct cgatccggga gcggctcagg agtcggaaga ctactgtgaa
      301 aaaccagcgc agcaggcgat ggcccacaag accctcgatt gtagacgcaa gaccggggtg
      361 ttacaatccg cgccatcctc ggtcatcgtc gcggtgttgc tcatttgaaa caccgcattt
      421 tcattcctcg gtctggagca ccggcctgcc aggtcagaag cctatgaagg ccgccattcc
      481 atgatgcccc tggctccgcc ccgacccgtc catccctggc tcaagaaact cctggtcgct
      541 tttgttccag gcgagaccag ctcgctcatg gagcaggtcg tcgacggatt gctggagcag
      601 tttcaatcgc tcgggcacca ggtccgaact tcgcctgagg gtcgaaccga tctggtgctg
      661 accaccgctc gtttcggcga gccgctcccc tggcgcaaag cgctgttctt caccgcccgc
      721 aagcgattcg ggctggagaa caacccggca atttatactc tcgttcaagt cgggactcgg
      781 gagtaccagg ccgccctcga tcactttcag gccgccctgg agcgggagcc tccgaatccc
      841 gcggattttg agttccctgg cctggcgccg gactcgaacc gggtgctgct cgagcagagc
      901 cggcgggggg gtccgatcct ggccctcgag cgccaggtcc aggcccaggc caagagcatc
      961 cgggtcctgc tgatcatcgg cgacgaccgg ccgctctggg cccatcactt tgacctggtc
     1021 ggggcccatc cgcggattga tatggtcgag gggaacgggt tctatcaaga cctcgcgcta
     1081 cgaatggtga ccgtgctgag caccgaggaa gtcaccgatc acgaagtcgt ggccgggctg
     1141 gtcccgcagg ccgagtgggc ggccctcgag gcgcccaagg ggatgttgtt ggccgggtca
     1201 gagcttggcc ggcggggatt tttcaccgag atggttcgga tctcagacct ggttgcagtg
     1261 ccggcagtct cggagggcgt ggccaatcag tatagtgaag gctgctttgc aacctgggat
     1321 ccaaagcttg gcgctctgat cgcgacggtg acaggcagcg cccggccggt tgacaaggac
     1381 gccatcgggg agaacgatct ggcagtgatc gtcggagtgc ggccggacgg caaaggtgcc
     1441 ctggtgcggc acgtggccgg gaagcgcaat gaccctccct cttccgaggc ggtcgagatg
     1501 atggagatgg acagcctgct tccgaagatt cggctcgata agaagtggga aacccaagcc
     1561 acggtcccgg tcgtgcgctc caagctacat gggcaccggg gcattgcggc ctacgatccg
     1621 gctcgggtcg agcatgtggc gctggagcct acctactatc actacccggt gtcgtgcgcc
     1681 acgcaggcgc aggcgtgggc gatcaaagat gcattcgccc ggtctgctgc gctacaggac
     1741 actgctgacc cgcggcagtt agtgtttacc gtgctgcccg ggcatggggt ggtgatcgct
     1801 gaaaagtggc tgccggggaa ggcgcccttc caggctattt gggaggcgat tgacgccggt
     1861 gagctggaga tctcgaacaa agttcctcaa gggttgctgc gctatgtgcc cgggcccaac
     1921 gggcgcatga tcttgaagga aggttaaccc catccagctc gggagttagg ctacgagaac
     1981 aatcctccta cggtagccgt gccgagtccg agcgcagcga acactagccc ggccacgaac
     2041 gtaatggcca gccctgcgat aaacgcaagc acaacggtga cgaccacctg aacccagtcg
     2101 aggtccaagg cctctttggc cgccacgaac caggccacta ggccaagcag cgcggctgcc
     2161 agacgggccg gcgcgacgac gcaggagaga gttgtcgata gtgccccaag cacgccaggc
     2221 acgccgaccc cgttcc
//
LOCUS       DATDPQ010000192         6154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3754535, whole genome shotgun sequence.
ACCESSION   DATDPQ010000192 DATDPQ010000000
VERSION     DATDPQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754535"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VI520_04505"
     CDS             complement(<1..332)
                     /locus_tag="VI520_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37178.1"
                     /translation="MFSGPRLAGAALALLTLACSLASPPVPTTTLEPAAALTSTALPS
                     PTPAPTRSELISAGQQALFNGDWDQAYVEFSRRLEQAGGPEEIAEALYGRGEALLQSG
                     LYAGAQVE"
     gene            440..1510
                     /gene="mtnA"
                     /locus_tag="VI520_04510"
     CDS             440..1510
                     /gene="mtnA"
                     /locus_tag="VI520_04510"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HLF37179.1"
                     /translation="MRTVSWDPTLSAVKMIDQRRLPAEYSIVTLESAAAVAEAIHDMV
                     VRGAPAIGASAAFGLALAAARSTASSSEQLWKDLRAAEQVLRASRPTAINLGWALDRM
                     LALAQAHSNPDPTALRGLLVAEAQRIADQDVDINRRMGSYGAALVQDGDTLIHHCNTG
                     ALATVDYGTALGVLRTAHENGKAIHVLVDETRPRLQGARLTAWELDQYGIEHEIIADN
                     AAGSFLQAGQVASVFVGADRVAANGDVANKIGTYMLALAAYDNRVPFYVVAPTSTIDL
                     SVGSGAEIPIEERPAAEVLNLQVDGEPIAPAGSRARNPAFDITPHRLVTAIVTEVGVL
                     RPPYELVLAEAVNSARSLWTSS"
     gene            1495..2451
                     /locus_tag="VI520_04515"
     CDS             1495..2451
                     /locus_tag="VI520_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HLF37180.1"
                     /translation="MDVVLTGSIAFDYIMRFPGRFGEHILPNRLDSLSLSFLVDTLDK
                     RRGGIAANIAYSMALLGERPRIMATVGEDFEPYGRFLQGIGVKTDEIKIIPGIHSASF
                     FVTTDAVDAQIASFYTGAMSHAAELKVADLDPRPELMLISPNDPRAMVSYASECQQLG
                     IPYFYDPSQQILRLEGAELERGIAGAEALFVNDYELALILDKTSLTLARIAERTRFTV
                     ITRSELGSELITPEGRVQIPAVRPRGAAEPTGVGDAYRAGFLKGYIHQFPLECCGRMG
                     ALAATYCLESLGPQGQQYDLSEYVSRFEQEFGSECGLKQRLV"
     gene            2467..3729
                     /gene="ahcY"
                     /locus_tag="VI520_04520"
     CDS             2467..3729
                     /gene="ahcY"
                     /locus_tag="VI520_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLF37181.1"
                     /translation="MSDDYDIRDAGLAEGGRRRITWAELEMPVLRQIRERFQKERPLK
                     GLRVSACLHITAETANLMRTLQAGGADSVLCASNPLSTQDDVAASLVVHEEIPVFGIK
                     GENRDTYYTHITAALDHRPTATMDDGADLVSSLHKERSGQLSEVVGGTEETTTGVIRL
                     RAMAKAGKLGYPILAVNDAMTKHFFNNRYGTGQSTIDGILRATNILLAGKTFVVAGYG
                     WCGRGLAMRARGLGSNVIVTEVDPLPALEAVMDGFRVMPMDEAARLGDIFCTLTGDVH
                     VLDRQHFMVMKDGAILANSGHFNVEINLEALEGMAGEKRKVREFVDQYLLDDGRRLNL
                     LAEGRLINLAAAEGHPASVMDLSFANQALSLEYIMQHATELDDQVYTIPEEIDREIAR
                     LKLETMGIRIDSLTSEQARYLTSWEEGT"
     gene            complement(3743..4264)
                     /locus_tag="VI520_04525"
     CDS             complement(3743..4264)
                     /locus_tag="VI520_04525"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HLF37182.1"
                     /translation="MRRAAEGLSWGYSALFGLWLSGCGTSPAATPLPTELIGPEPLAT
                     STHVPSTPAPPVALTQSGCVDQARFIEDLSVPDGSRVEPGALIDKRWSVMNSGTCNWG
                     PGYRLVRTDGGSLGGPAELALYPARAGEAAIWQVLLTAPREPGEHLASWQAKTPTGEL
                     FGDPVFVLVEVRP"
     gene            complement(4322..5407)
                     /gene="recA"
                     /locus_tag="VI520_04530"
     CDS             complement(4322..5407)
                     /gene="recA"
                     /locus_tag="VI520_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLF37183.1"
                     /translation="MDNGKAGPNERLEVLGGALGEIVKRFGEGAVIRLGEATHLAVQA
                     IPTGSLPLDLALGVGGIPRGRVSEIYGPEASGKTTLCQHIVAESQRLGGLVAYIDMEH
                     ALDPAYAALCGVDIDNLYISQPDTGEQALEITETLVRSGAIDVVVVDSVAALVPRAEI
                     EGDMGEPTMGLQARLMSQALRKLSGAIKQTNTAVVFTNQLRQRIGVMFGNPETTTGGM
                     ALKFYASVRIDVRRIESIKVGQEIVGNRVRARVVKNKVAAPFRVAEFEILFNEGISKV
                     GGILDLAVENGLVEKRGSFFSVGETRIGQGRDNAKNYLREHPEMMLDLESRIRGLSGL
                     AFVSSEGESEEEGRPAAIAEPRLAYAA"
     gene            complement(5442..>6154)
                     /locus_tag="VI520_04535"
     CDS             complement(5442..>6154)
                     /locus_tag="VI520_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37184.1"
                     /translation="DETVNHSNRQMETVIEEALRLRCERVDVFFLIGLPQQSQESVRE
                     TIGYCEHLFQISDKRLSCFISPLGPFLDPGSRGFEDPARVGYRLFARTLEQHRQLLVQ
                     PTWKRILNYETRWMTREQLVEATYEAGEQLNALKVRYRRISRIRGEQVARRIWLARGL
                     RDRLDELVDRGTTEGDEFERLRGEVHAFSVSTVCDKRELFWRRHLFNFKILTILRIAF
                     SYLVEQIAHLTNRTTVLE"
BASE COUNT         1137 a         1983 c         1907 g         1127 t
ORIGIN      
        1 aattcaactt gggcgccggc gtacagtccg gattgcaaca aggcttcccc tcgcccatag
       61 agggcctccg cgatctcttc cggtccgccg gcttgctcaa gccgccggct gaattcaacg
      121 tacgcctggt cccaatctcc attgaacagc gcctgctggc cggcactgat cagctccgag
      181 cgagtaggtg ctggagtcgg agagggaagg gcagtcgagg ttagggcggc ggccggctcg
      241 agggtggtgg taggaaccgg gggagaggcc aggctgcagg cgagggtcag cagcgcaagc
      301 gccgcgcccg cgaggcgagg gccggagaac acggccggca tcatatcacc attcccttgc
      361 aagccgtctg tgggcgtgct agactttccg tgcagttcaa ctgcgctctc ggcggagagc
      421 gcgtttttgt gccttgccgg tgagaaccgt atcttgggac ccaaccctct cggcagtcaa
      481 gatgatcgat cagcggcgct tgccggctga gtactcgatc gtcaccttgg aatcagcagc
      541 agccgttgcc gaggcaatcc acgacatggt agtccgcggc gctccggcca tcggggctag
      601 cgccgctttt ggtttggcgc tggctgctgc tcgatcaacg gcctcgagct ccgagcagct
      661 ctggaaggac ctgcgggccg ctgagcaggt gctgcgggcc tcccgtccga ccgcaatcaa
      721 cctcgggtgg gcgctcgacc gaatgctggc cctagcccag gctcactcga atccggaccc
      781 gactgcgctg cgcggcctgc ttgtcgctga ggctcagcgc atcgcagatc aagacgtcga
      841 catcaatcgg cggatgggca gttacggagc ggctctggtt caggatggcg acaccctgat
      901 ccaccactgc aatacgggtg cgctggccac cgtggactac ggcaccgccc tgggggtcct
      961 gcgcactgcc cacgaaaacg gaaaggcgat ccatgtcctg gtcgacgaga ctcgcccgcg
     1021 cttgcaaggg gcgcgattga ccgcttggga gcttgatcaa tacgggatcg agcatgagat
     1081 catcgccgac aatgctgccg gctcattcct gcaggccggc caagtggcgt cggtatttgt
     1141 gggagccgac cgggtcgccg ccaacggcga tgttgcgaac aagatcggga cctacatgct
     1201 ggcgctggct gcttacgaca accgggtccc gttctatgtt gtggcgccca cttcgacaat
     1261 tgatctctcg gtgggcagcg gcgcggagat cccgatcgaa gagcggcccg ccgccgaggt
     1321 gctcaacctc caggtcgatg gggagcccat agcgcccgcg gggagccggg cgcgcaatcc
     1381 ggccttcgac atcaccccgc atcgcttggt gaccgccatt gtgaccgagg tcggcgtcct
     1441 ccggccaccc tatgagttgg tacttgcgga agccgtcaat tcggcccgga gcctgtggac
     1501 gtcgtcctga cgggatcgat cgccttcgat tacatcatgc ggttcccggg ccggttcggc
     1561 gaacatatcc tgccgaaccg gctcgacagc ctcagccttt ccttcctggt ggataccctc
     1621 gacaagcgca gggggggcat cgcagccaat atcgcctact cgatggcgtt gttgggtgaa
     1681 cgcccgcgga tcatggcgac agtgggcgaa gacttcgagc cctatggtcg tttcttgcag
     1741 gggatcgggg tgaaaacgga cgagatcaag atcatccctg gcattcactc tgcctccttc
     1801 tttgtcacga ccgacgccgt ggacgcgcag atcgccagct tctataccgg ggcaatgtcc
     1861 cacgcagcgg agctgaaggt ggccgacctt gacccgcgcc ccgagctgat gctcatctcg
     1921 ccgaacgacc cgcgtgccat ggtcagctac gcctccgaat gccagcaact cgggattccc
     1981 tatttctatg acccgagtca acagatcctt cggcttgagg gcgcggagct ggagcggggg
     2041 atcgcgggtg cggaggcgct gttcgtcaac gactacgaac tcgctctgat cctggacaag
     2101 acctccctta ctctggcccg gatcgccgag cggacccggt tcacggtcat tacccgcagc
     2161 gagctgggat ccgagctgat cacgccggaa ggtcgggtcc aaatccccgc cgtgcgaccg
     2221 aggggagcgg ccgaaccaac cggggtcggg gacgcctatc gggctggttt cctgaaggga
     2281 tacatccacc agtttccgct cgagtgctgc ggtaggatgg gtgcgctggc cgccacgtac
     2341 tgcctcgaga gtctggggcc gcagggacag cagtatgacc tgagtgaata tgtctctcgg
     2401 ttcgagcagg aattcgggtc agagtgcggg ctcaaacagc gactggttta agagcggagg
     2461 agactaatgt ccgacgatta tgacattcga gacgccggcc tggcggaagg cggacgcagg
     2521 cgcattacat gggccgagct ggaaatgccg gttctcaggc agattcgaga acgcttccag
     2581 aaggagcgtc cgctcaaggg cctgcgagta tcagcctgcc tgcacatcac ggccgagacc
     2641 gcaaacctga tgcgaacctt gcaagcgggg ggtgccgatt ccgtgctgtg cgcctcaaac
     2701 ccactttcca ctcaggacga cgtggccgcc tcgctggtcg tgcatgagga gatcccagtt
     2761 ttcgggatca agggcgaaaa ccgggacacc tactacaccc acatcacggc cgccctcgat
     2821 caccggccga cggcgacgat ggacgatggg gcggacttgg tgagctcgct gcacaaggaa
     2881 cgttccggcc agctctcgga ggtggtgggc ggtaccgagg agaccaccac cggcgtgatc
     2941 cggcttcggg cgatggccaa agcgggcaag ctcggctatc cgatcctggc agtgaacgac
     3001 gcgatgacca agcacttctt caacaaccgc tatggaacgg gccaatccac tatcgacggg
     3061 attctgcggg ccacgaatat tctgctggcg ggcaagacct ttgtggttgc ggggtacggc
     3121 tggtgtggcc ggggtctggc gatgcgggcc cggggacttg gatccaatgt aatcgtcacc
     3181 gaggtggatc cgctgccggc actggaggcc gtgatggacg ggttccgggt gatgccgatg
     3241 gacgaggccg caaggctagg cgatatcttc tgcacattga ccggcgacgt ccatgtcctc
     3301 gaccggcagc atttcatggt catgaaagac ggagcgatcc tggcgaactc cgggcacttc
     3361 aatgtcgaga tcaatctgga agccctcgaa gggatggcag gcgagaaacg gaaggtccgc
     3421 gaattcgtcg accaatacct tttggacgac gggcgccggc tgaatctgct ggccgagggc
     3481 cgcctgatta acttggcggc ggccgaaggc cacccggcca gcgtgatgga tctttcattt
     3541 gcgaatcagg ctctctcgct cgagtacatc atgcagcacg caaccgagct ggacgaccag
     3601 gtctacacaa tccccgagga gatcgaccgg gaaatcgccc gcttgaaact tgagacgatg
     3661 gggatccgga tcgattcgct aacctccgag caggcccgct acctgacttc ctgggaagaa
     3721 ggtacatagc cgaggccgac cctcagggcc tgacctcaac caagacgaat accggatcgc
     3781 cgaacagttc accagtcggc gtcttggcct gccagctggc gaggtgctcc cccggctcgc
     3841 ggggtgcggt cagcagcacc tgccagattg ccgcctcgcc agcccgagcg gggtacagcg
     3901 caagctcggc cggaccgccg agcgatccgc cgtcggtccg gaccagtcgg tatccaggcc
     3961 cccaattgca ggtccccgaa ttcatgaccg accagcgctt gtcgatcagc gcccccggct
     4021 ctacccggct tccatcgggt acggagagat cttcgatgaa gcgcgcctgg tccacgcatc
     4081 cggattgagt cagtgcgacc gggggtgcag gagttgacgg aacatgggtg ctcgtcgcga
     4141 gcggttctgg cccaatgagc tccgtcggaa gcggagtcgc agccggcgac gttccacaac
     4201 cggagagcca caggccgaac agtgcagagt acccccaact caaaccctcg gccgctcgcc
     4261 tcatcaaccc aatcatcgcc cgatccggag agcggggcaa gccgggcgct ggctctggat
     4321 gctaggcggc gtaggccaat cgcggttctg cgattgccgc cgggcggccc tcttcttcgc
     4381 tctcgccctc cgagctgacg aaggccagcc cgctcagacc ccggatccga ctttcgagat
     4441 ccagcatcat ctcagggtgc tcccgcaagt agttcttggc gttgtccctg ccctgcccga
     4501 tccgggtttc gccaaccgag aagaatgacc cgcgcttctc caccaagcca ttctcgaccg
     4561 ccaggtccaa gatcccccca accttgctga tcccctcgtt gaacaggatc tcgaactcgg
     4621 cgacccggaa cggggcggcc accttgttct tcaccactcg ggcccgcacc cggttcccca
     4681 cgatctcttg cccgactttg atgctttcga tccgccgtac gtctatgcgc accgaggcgt
     4741 agaatttcaa ggccatcccc ccggtggtcg tctccgggtt gccgaacatc accccgatcc
     4801 gctgccgcag ctggttggtg aacaccaccg cggtgttggt ctgcttgatc gctccggaga
     4861 gcttgcgcag ggcctggctc atcagccggg cctgcagccc catggtcggc tcccccatgt
     4921 ccccctcgat ctcggcccgc ggcaccagcg ccgccacact gtccaccacc accacgtcga
     4981 tcgctccact gcgcaccagg gtctcggtga tctccagcgc ctgctcgccg gtgtccggct
     5041 gggagatgta gaggttgtcg atgtccaccc cgcagagggc ggcgtaggcc gggtccaaag
     5101 cgtgctccat gtcaatgtag gccaccagcc cgcccaaccg ctgggactcc gccacgatat
     5161 gctggcaaag ggtggtctta ccggaggctt ccggcccata gatctcgctc acccgcccgc
     5221 ggggaatgcc gccgaccccc agggccaggt ccaagggcaa ggatccggtg gggattgcct
     5281 ggaccgccag atgggtggcc tcccctaacc ggatcacggc gccttccccg aagcgtttga
     5341 cgatttcccc caacgctcca ccaagcactt ccagtcgttc attgggtcct gcctttccat
     5401 tgtccacttc gctaccgtcc tctctagaac gtatgttcgg tctattctag cacagtagtt
     5461 ctattcgtca agtgggcaat ttgctcgacg aggtaggaaa aggcgatccg caggatcgtg
     5521 aggatcttga agttgaacag atgccggcgc cagaaaagct cccgcttgtc gcaaaccgtc
     5581 gacacgctga aggcgtgaac ctcgccccgc aggcgctcaa actcgtctcc ttcggttgtt
     5641 ccccgatcga ccaactcatc cagacgatcg cgcaaccctc tggcgagcca gatccggcgg
     5701 gccacctgct cgccgcggat ccgagagatc cgccggtagc gaactttgag cgcgttcaac
     5761 tgttcacccg cctcataggt ggcctcaacc agctgctccc gggtcatcca gcgggtctcg
     5821 tagttcagga tccgtttcca ggttggctgg accagcagct ggcggtgttg ttccagggtt
     5881 cgggcgaaca gacgataacc cacccgcgcc ggatcctcga acccccggct tcccggatcg
     5941 aggaacgggc ccaatgggga gatgaaacac gacagacgct tatccgagat ctggaagagg
     6001 tgttcgcagt agccgatcgt ctcacggacc gattcctggc tctgctgtgg gaggccgatc
     6061 aggaagaaga catccacccg ttcgcagcgc agccgcaatg cctcctcgat cacagtctcc
     6121 atttgacgat tgctatggtt gacggtctcg tcct
//
LOCUS       DATDPQ010000193         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2958092, whole genome shotgun sequence.
ACCESSION   DATDPQ010000193 DATDPQ010000000
VERSION     DATDPQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958092"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VI520_04540"
     CDS             <1..313
                     /locus_tag="VI520_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HLF37185.1"
                     /translation="LGTALNLKPILELQDGVIEAVERVRTRSKAQARLLGLLEERING
                     RPNLRLAVLHAAAEREAHQLLDEAVKKLNPMETVFSEVSPVVGAHVGPGTLGLCYSVD
                     L"
     gene            complement(321..815)
                     /locus_tag="VI520_04545"
     CDS             complement(321..815)
                     /locus_tag="VI520_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HLF37186.1"
                     /translation="MAWLLAVLTAACSAPPGVGTVEIRLSDHREAIGDFARLTVEIDR
                     FELHPASASPESGWIVLKPAITEADLTQLIGEASLPVVSQAVAAQSYDAVRLVLSGAI
                     GILNDGAEIEFGEFSEAGRVEFELAEAGRVTILIDLVVQSRLDHPGEGYLILLGETKP
                     VAGD"
     gene            complement(886..1785)
                     /locus_tag="VI520_04550"
     CDS             complement(886..1785)
                     /locus_tag="VI520_04550"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLF37187.1"
                     /translation="MPSNRIWFRVLLGIATGLAVVGAVFTARLLTTPTSPEEVAEAFF
                     RAGYRHDYGSAWELVSSQDQVARSKEAYLAANPLPNDQQTAVYDQLAELGEFAVLAIA
                     STRPEQAILTAQVRFPNSGKPEIQELIGRAADPSANRSELQQQLDGLRDAGQLGFFEG
                     EVSFNLVLERDRWRVVQHWGNAIAIQLEAAVDPGLPWEFYPIQSEVLAQPGELVSASY
                     MAINNSSESITAKAIHEVGPAEASGYFQTIQCFCFTEQTLEPGEQREMQLLFRIDFTL
                     PPGIELFRNRYTFYALEDFPADG"
     gene            complement(1797..>2627)
                     /locus_tag="VI520_04555"
     CDS             complement(1797..>2627)
                     /locus_tag="VI520_04555"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLF37188.1"
                     /translation="GALLDRVSGRAMNDAVTTFTKQLIAIGLLAAAGSYAAAFIDLRR
                     HNRLAGLRSRALIRDFHPVLFGVGLAAIAIALLSPLDSLAGVRFSAHMVQHLLLIMVA
                     APALLLGLPSPLARGALGKRWIRQLLDRVSDPLIAYFAFNANLLLWHIPRLYQLALVN
                     PWIHDLQHALFFYTAVLVWWRVIDPTHGWFPLWRWAPARWAYLLVFAPPSYALGAVLW
                     GGGWLLYPYYAAVGAMFQLTALADQRLGGLFMWIQGWMFLMLSMFVFYARYDPELEEA
                     "
BASE COUNT          540 a          814 c          841 g          432 t
ORIGIN      
        1 gcttgggacc gcgctcaatc tcaagccgat cttggagctt caggacgggg tgatcgaggc
       61 ggtcgaacga gtgcgaaccc gttcgaaggc ccaggcccga ttgctcggcc tgttggagga
      121 gcggatcaac ggccggccta atctgcggct ggcggtgctc cacgcagcgg ccgaacggga
      181 ggcccaccaa ctgctggatg aggcggtcaa gaagttgaat ccgatggaga cggtgttctc
      241 ggaagtcagt ccggtggtcg gagcccacgt gggccccggc accctgggat tgtgctacag
      301 cgtcgatctg tagcgatcag tcagtcgccg gcgaccggct tggtttcccc cagcaagatt
      361 aggtagccct cgccggggtg gtcgagccgg ctctggacca ccagatcgat caagatggtc
      421 acccgcccgg cctcggccag ttcgaactcg acccgtccgg cctccgagaa ttcgccgaac
      481 tcgatttcgg ccccgtcgtt caatatccca atcgcgcccg agagaactag ccgaaccgcg
      541 tcgtagcttt gcgcggcaac ggcctggctg acgaccggga gggaagcctc cccgatgagc
      601 tgggtcaggt cggcttccgt gatcgccggc ttcaatacaa tccacccaga ttcgggcgac
      661 gcagaggccg ggtgcagttc gaaccggtcg atctcaacgg tcaaccgggc gaagtcgccg
      721 atcgcctccc ggtgatcgga caaccggatc tcgaccgtgc caactccggg tggagcggag
      781 caggcggccg tgagcacagc caatagccag gccataccgc gttcgaccgc gattcggcga
      841 ctcgattggc cactcaaatt gggggaccgt ccgtccggct ttctctcagc cgtcagcggg
      901 aaaatcctcg agcgcgtaaa acgtgtagcg attgcggaac agctcaatgc ccggagggag
      961 ggtgaagtcg atccggaaca gcagctgcat ctcgcgttgt tccccgggct cgagggtctg
     1021 ttcggtgaag cagaagcact ggatggtctg gaagtagccg gacgcctctg ccggcccgac
     1081 ctcgtggatc gccttggccg taatcgattc gctcgagttg ttgatcgcca tgtagctggc
     1141 gctgaccaat tcccccggct gggctaagac ctccgactgg atcgggtaga actcccaggg
     1201 gagtccaggg tcaaccgcgg cctcgagttg aatggcgatg gcgttgcccc agtgctggac
     1261 caccctccag cggtctcgtt ccagaactag attgaaactg acctcgccct cgaagaaacc
     1321 cagctgcccg gcgtcgcgca gcccatcgag ctgctgctgc agctccgatc ggttcgcgga
     1381 cgggtccgcc gcccgcccga tcaattcttg gatttcgggt ttaccgctgt tcgggaaacg
     1441 gacctgggcg gtcaagatcg cctgttcggg ccgggtggag gcgatagcca gcaccgcgaa
     1501 ctcgcccagc tcagccagtt gatcgtagac tgccgtctgt tgatcgttgg gcagcggatt
     1561 agcggccaga taagcctcct tactgcgtgc cacctggtcc tgcgagctga ccaactccca
     1621 ggcggaccca tagtcgtgcc gatagccggc tcggaagaaa gcttcggcaa cttcctccgg
     1681 tgaggtaggt gtcgtgagca gccgggccgt aaagactgcc ccgacgaccg ccagaccggt
     1741 cgctatcccg agcagcactc gaaaccagat tcgattagag ggcatgcggc ctcgacttac
     1801 gcctcttcaa gctcggggtc gtaccgagcg tagaacacga acatgctcag catcaagaac
     1861 atccagcctt gaatccacat gaacaaccca cccagccgct ggtcggccag cgcagtcagc
     1921 tgaaacatgg cgccaaccgc tgcgtaatac ggatagagca gccagccgcc gccccaaagc
     1981 acggctccca aagcgtagct cggtggggca aagaccaaaa ggtacgccca gcgggccgga
     2041 gcccagcgcc agagcggaaa ccacccgtga gtcggatcga tgactcgcca ccataccagc
     2101 acggcagtgt agaagaacaa agcgtgctgg agatcatgga tccacggatt gaccagcgct
     2161 aactgataga gtcttggaat gtgccagagg agcagattgg cattgaaggc gaaataggca
     2221 atcagtggat cgctgacccg atcgaggagc tgccgaatcc agcgcttgcc cagcgcgccc
     2281 cgggcaagtg gggagggcag gccgagcagc agcgcgggcg cggcaaccat gatcagcagc
     2341 aggtgctgga ccatgtgggc cgagaatctc acgccggcca agctatccag cggagagagc
     2401 agtgcaattg caatggcggc caaacccacc ccgaacagga ccgggtgaaa gtcgcgaatc
     2461 agggcgcgcg agcgaaggcc agccaaacgg ttgtgacgcc gcaggtctat gaaggccgcc
     2521 gcgtaggatc cggccgcggc cagcaggccg atcgcgatca gctgtttggt gaaggtggtg
     2581 acggcgtcgt tcatggcccg gccggagacc cgatcaagta gagcgcc
//
LOCUS       DATDPQ010000194         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2191237, whole genome shotgun sequence.
ACCESSION   DATDPQ010000194 DATDPQ010000000
VERSION     DATDPQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191237"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VI520_04560"
     CDS             complement(<1..495)
                     /locus_tag="VI520_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37189.1"
                     /translation="MKVPLSWLREFVEIEISIMELAQRLTVAGLEVEEIRHVGLPFEG
                     ASPVSRQETRVSGLAWDPERIVVGEIREVMPHPNADRLVLTRLYDGEQEHTVLTGAPN
                     LFGFKGTGPLERPLKVAYAKAGAEIYDGKQAGWEKMILKPAKIRGVESSSMACSEKEL
                     GISDD"
     gene            complement(510..1544)
                     /gene="pheS"
                     /locus_tag="VI520_04565"
     CDS             complement(510..1544)
                     /gene="pheS"
                     /locus_tag="VI520_04565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLF37190.1"
                     /translation="MQQMIDQLAQIEREALAQLGAIRTSADLQSWKVSHLGRSAPLAV
                     ILDGLRELPKDQRPAVGRRANEVKAGLEHAFAAREETLRREELDRSLAEQRIDVTLPG
                     RRPNRGRIHPANRTVREIIHIWAEMGFQVYRSREVETDDYNFGLLNMPRHHPAREMQD
                     TFYTTDPEVLLRTHTSPGQIRVMRERAPEPIRVVLPGMCFRYEQITVRSEIQFNQIEG
                     LAVGHHITMADLKGTLSAFARRTFGQNVRTRFRASYFPFTEPSAEMDVECFVCGGAGC
                     PVCKRTGWLEILGSGMVHPTVLQNGGYDPELFSGFAFGMGPERITMLKHGIEDVRHFW
                     GNDLRFLAQF"
     gene            complement(1635..>2238)
                     /locus_tag="VI520_04570"
     CDS             complement(1635..>2238)
                     /locus_tag="VI520_04570"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HLF37191.1"
                     /translation="IGGYLLGSIPIAWIVTRLVTGEDLRSLGSGNVGVMNVALSTTRW
                     AGLVVLVGEAAKGLLAVILARRVSDSPLAIGITVLAAVIGTRWMVWLGFAGGRGNTAG
                     LAAMALLSWQTLLIGIGIWALLRLITRSSFWATRISLVSWPFVFGWLTQSWIYLIFGL
                     VLSGIYLTTHRTVSDDHTIIKERWPSLWAFLTGPKRERPS"
BASE COUNT          417 a          754 c          667 g          400 t
ORIGIN      
        1 gtcatccgag atgcccaact ccttttccga gcacgccatc gacgacgact cgactccccg
       61 gatcttggcc ggcttcagga tcatcttctc ccagccggcc tgcttgccgt cgtaaatttc
      121 cgcacctgcc ttcgcgtagg ccactttcaa gggccgttcc aggggcccgg ttcctttgaa
      181 cccgaacagg ttcggcgctc cggtcaggac cgtgtgttct tgctcgccat cgtagagccg
      241 ggtcaacacc aaccgatcgg cgttggggtg gggcattacc tcccgaatct cgccgaccac
      301 gatccgctcc ggatcccagg ccaatccgga gacccgggtc tcctgccggg atacggggga
      361 agctccctcg aaaggcaatc cgacgtggcg gatctcttcc acttccagcc cggccacggt
      421 caggcgctgg gcgagctcca tgatcgagat ctcaatctcg acgaactcgc gcaaccaaga
      481 gagagggacc ttcatgcctc gctgctcagc tagaactgcg ccagaaagcg caggtcgttg
      541 ccccagaaat ggcggacgtc ttcgattccg tgtttcagca tcgtgatccg ctccggtccc
      601 atcccgaacg cgaatcccga gaagagttcc gggtcgtagc cgccgttctg caagaccgtc
      661 gggtgcacca tgccgcttcc caggatttcc agccagccgg ttcgcttgca gaccgggcag
      721 ccggcgccgc cgcacacgaa acactcaaca tccatctcgg cggacggctc ggtgaaaggg
      781 aaatacgagg cccgaaaccg ggtgcgcacg ttctgtccga aggtgcgtcg agcgaaggcg
      841 ctgagggtgc ccttcaaatc ggccatcgtg atgtggtggc cgaccgccaa tccctcgatc
      901 tgattgaact ggatctccga gcgaaccgtg atctgttcgt accggaagca catccccggc
      961 agcaccaccc gaatcggctc cggggcccgc tcgcgcatca cccggatctg gcccggcgaa
     1021 gtgtgggtgc gcagcagcac ttccgggtcg gtggtgtaga aggtgtcctg catttcgcgg
     1081 gccgggtggt ggcgcggcat gttgagcagg ccgaagttgt agtcgtcggt ctcgacctcg
     1141 cgggagcggt agacctggaa ccccatctcg gcccagatgt ggatgatttc acgcacggtc
     1201 cgattggccg gatggatccg gcctcggttt ggacgccggc ccggcagggt gacatcgatc
     1261 cgctgctcgg cgagcgaccg atcaagttcc tcccgccgca gcgtctcttc ccgcgccgca
     1321 aaggcgtgct ccagtccggc tttgacctca ttcgcccgcc ggccgactgc cggtcgttga
     1381 tccttgggca gctcgcggag cccgtccaga atcaccgcca gcggcgccga gcgaccgagg
     1441 tggctcacct tccaggactg cagatccgcc gaagtgcgaa ttgcccccaa ctgggccagc
     1501 gcttcccgct cgatctgggc tagctgatct atcatctgtt gcattctaga atcaaaactc
     1561 ccgccctcca agggacggga gcctcgccca tcggggatta tctccgaatc aattggcttg
     1621 tcaacggcgt gagctcaaga tggccgctcc cgcttcggac cggtcaggaa agcccacaaa
     1681 ctgggccagc gttccttgat gatcgtgtga tcgtcgctga cggtgcgatg ggtagtcaga
     1741 tagatcccac tgagcaccaa cccgaagatg aggtagatcc aggattgggt cagccaccca
     1801 aagacgaatg gccagctcac tagactgatc cgagtcgccc agaaagagct gcgggtgatc
     1861 aggcgcagca gggcccaaat cccgatccca atcaggagag tttgccagga tagcagcgcc
     1921 atcgcggcca gtccggcggt gttgccgcgc ccaccggcga acccgagcca caccatccag
     1981 cgggtgccaa tcacggcggc caagaccgtg atcccgatgg ccagcggact gtcactgacc
     2041 ctgcgggcca ggatcacggc cagcagccct ttggccgcct cgcccacgag cacgactagg
     2101 cccgcccagc gggtagtact cagtgccacg ttcatcactc ccacattccc gctgcccaag
     2161 gatcgcaggt cttcgccggt caccagccgg gtgacgatcc aggcgatcgg gatcgatccg
     2221 agcaggtagc caccgatc
//
LOCUS       DATDPQ010000195         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_600582, whole genome shotgun sequence.
ACCESSION   DATDPQ010000195 DATDPQ010000000
VERSION     DATDPQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600582"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VI520_04575"
     CDS             <1..685
                     /locus_tag="VI520_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HLF37192.1"
                     /translation="SYASPVLERRVRPLAESVGSNFVELCDVTDDQQIEAVFAKASDQ
                     FKQLDILVHAIAFANREDLEGGFYTTSREGFRIAMDVSVYSFVALARAAQALMPDGGS
                     MMTLTYYGGQKVVANYNVMGVAKAALEAATRYLAADLGPKGVRVNAISAGPVRTLSAA
                     GVRGFKSLYREFAELAPMRRQISAEDIGGAAVWLASDLARNVTGEGLFVDSGYNILGV
                     PSVDASNRT"
     gene            666..1829
                     /locus_tag="VI520_04580"
     CDS             666..1829
                     /locus_tag="VI520_04580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLF37193.1"
                     /translation="MRQTERESLIDRLRASLFPRIEPELRATRTSLARSVILVQFAVL
                     AFWGIALLVLSSLRPEVRVTLDLAAIAALAVSGAAALSLVKRGSVRTAGYLISFSTFA
                     VIAVGTFLIPRYFPLVAVAFLLPVLISGAVIGGWAPFLFAFSAIVINTTAVIIAAGRG
                     EAAVAENETWTLIYLVTHSLVSLAAALISNSLTEQIQKTIGSLHRQADQLAKLAHTDP
                     LTGLANRRHLFERLESEFSRAQRYRRPFCLLYIDMDGFKAINDQFGHLFGDEILRGSA
                     RAMQAVLRATDLLARIGGDEFAVLLPETDLTGSEHVANKLRKALAAYGSQLSPSLPSL
                     TLSIGVGQINEDDDAIEDILSRADKAQYLAKSSGKALTRTQQDLRTATPIEQA"
     gene            1885..>2473
                     /locus_tag="VI520_04585"
     CDS             1885..>2473
                     /locus_tag="VI520_04585"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLF37194.1"
                     /translation="MKVLLAVLAHPDDETFGMGGTLALYARRGAQVHLVCATRGEAGT
                     VSPEHLQGYENIAQLREAELRCAASHLGIAEVHFLGYRDSGMAGSPENQHPESLASAN
                     LKQVVERVTKHIRRTKPQVVITFDPAGGYGHPDHIVTHQATVEAFQASGDSARFPKAG
                     PAFAPQKLYFITFSRRFASLSLMLLRLSGHDPSRMG"
BASE COUNT          439 a          794 c          759 g          481 t
ORIGIN      
        1 ctcctacgcc agcccggtgc tcgaacggcg ggtacgcccg ctggccgaat cggtcggctc
       61 gaatttcgtc gaattgtgtg acgtcaccga cgatcagcag atcgaggccg tgtttgccaa
      121 ggcgagcgat caattcaagc aactcgacat cctcgtccac gcgatcgcat ttgccaatcg
      181 ggaagacctg gaggggggtt tctacacaac cagccgggaa ggctttcgga tcgcgatgga
      241 cgtcagtgtt tattcattcg tcgccttagc ccgggcggct caggcgttga tgccggacgg
      301 cggatcgatg atgaccctca cctattacgg cggccaaaag gtggttgcga attacaacgt
      361 aatgggagtc gccaaggccg cactggaggc ggcgactcgt tacctggccg cggatctggg
      421 ccccaaaggg gttcgggtca atgccatctc cgccggcccg gtccggaccc tctcggccgc
      481 cggagtgcga ggattcaagt cgctgtatcg tgaatttgcc gagctcgcgc ccatgcgccg
      541 ccagatttcg gccgaggaca tcggcggggc ggccgtctgg ctggcctccg acctggcccg
      601 caacgtcacc ggggagggcc tgtttgtcga ctctgggtac aatattctcg gagtaccatc
      661 cgtcgatgcg tcaaaccgaa cgtgaatcgt tgatcgaccg cctccgggct agtctgttcc
      721 ccaggatcga gccggaatta cgggcgactc ggaccagcct ggccaggagc gtgattctgg
      781 tgcagttcgc cgtcctggcg ttctggggga tcgccctgct ggtgctctcc agcctgagac
      841 cggaggtgcg ggtcaccctc gacctggcgg cgattgccgc gctggcagtg agcggggctg
      901 ccgctctgtc gttggtcaag cgcggatcgg tgagaaccgc ggggtacctg atctcgtttt
      961 ccacctttgc agtaatcgcg gtcgggacgt tcctgattcc ccgttatttc ccgctggtcg
     1021 cggtggcctt cctgctgccg gtcctgatct cgggcgcggt cattggaggc tgggctccct
     1081 tcctcttcgc cttctcggcc atcgtgatca acacgacggc ggtcatcatc gcggccggtc
     1141 ggggcgaggc agccgttgcc gaaaacgaaa cctggactct gatctacctg gtgacccatt
     1201 cgctggtctc gctggccgcg gccctgattt ccaactcgct gaccgagcaa atccagaaga
     1261 cgattggaag cctgcatcgt caggccgatc agctggcgaa gctggcccac accgatccgc
     1321 tgaccgggct ggcgaatcgg cggcacctct tcgagcgcct ggagagcgag ttctcccgcg
     1381 ctcagcgcta ccgccggccc ttctgcctgc tctatatcga catggacggc ttcaaagcca
     1441 tcaacgatca attcgggcac ttgttcggag acgagatcct gcgcggatca gcccgcgcca
     1501 tgcaggccgt gctgcgggca accgatctgc tagctcgaat cggaggggat gagttcgcgg
     1561 tgttgctgcc cgagaccgat ttgactggat cggaacacgt tgcgaacaag cttcgaaagg
     1621 cgctcgctgc atacgggagt cagctcagcc cctctttgcc ctccctcacg ctgtcgatcg
     1681 gggtcggaca gatcaacgaa gacgatgacg cgatcgagga tatcctgtcg cgagcggaca
     1741 aagcccagta cctggccaag tcctccggta aagccctgac ccgcacccag caagacttac
     1801 gaaccgccac ccccatcgag caggcgtaat tccgggagct ccccagatcc atgtggtatc
     1861 ctcgcccttc gcttcggagg tcgagtgaag gttctgcttg ccgtgctggc ccatcccgat
     1921 gacgagacct ttgggatggg aggaactttg gccctctacg cccgtcgggg cgcccaggta
     1981 catcttgtgt gcgcaacccg aggcgaggcc ggaacggttt ctccggagca tctgcaaggt
     2041 tacgagaaca tcgcccaact gcgggaggcc gagctgcgct gcgccgcctc tcacttggga
     2101 atcgcggagg ttcacttcct tggctaccgc gattcaggaa tggcgggcag ccccgagaat
     2161 cagcaccccg agtctctagc aagcgcgaat ctcaagcagg tggtcgagcg ggtcacgaag
     2221 cacatccggc ggaccaagcc ccaggtcgta atcacttttg atccggccgg cggctacggc
     2281 caccccgatc atatcgtcac ccaccaagca acggtcgagg ccttccaggc ctccggcgac
     2341 tcggccagat tcccgaaggc cggacctgcc ttcgccccgc agaaactcta tttcattaca
     2401 ttcagtcgtc gcttcgccag cctcagcctg atgctgctga ggctgagcgg acacgacccg
     2461 agccggatgg gcc
//
LOCUS       DATDPQ010000196         2777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1664208, whole genome shotgun sequence.
ACCESSION   DATDPQ010000196 DATDPQ010000000
VERSION     DATDPQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664208"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1234)
                     /locus_tag="VI520_04590"
     CDS             complement(128..1234)
                     /locus_tag="VI520_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLF37195.1"
                     /translation="MPDGWSWPFRCSSCGREYPREALPFRCAVCGGHYDLADPLRYAP
                     IPVPGLERYRNSFPLPPGARLLSLGEGGTPLVGQEIEGKRVFFKCEYLNPTGSFKDRG
                     TAVLISALAAAQVREAIEDSSGNAGASFAAYAARAGIRAKVFMPDSASGPKRAQIEAY
                     GAEVVSILGPRSEAAQAVVRAAEAGSVYASHAYMPQILAGMATFAFELVEQLGRAPGA
                     LLLPVGQGTLLLGAYLGFQALARQKLIHQLPQLIGVQARACAPLWAVHEAGMAGLGWV
                     QEGQTLAEGIRILNPLRGDAVLAAVDATQGGVEVVEESEIPAGRRALAERGFFVEPTS
                     AVVWSALLRRLPDLPEPIVAVLTGSGYKSAWNGA"
     gene            complement(1237..1944)
                     /gene="moaD"
                     /locus_tag="VI520_04595"
     CDS             complement(1237..1944)
                     /gene="moaD"
                     /locus_tag="VI520_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HLF37196.1"
                     /translation="MQIEILYFATLRDLAGLRREQLSLPEHSTVGDLRNRLSERGDRL
                     ALALGSALFSVNHEFAFPEEGLNEGDEVGVFPPVSGGAHPTLLKVTEEPLDLNNLIES
                     IAGASTGAVCIFSGLVRAQTERGEPRLTSFLEYEAYWPMAEAKLNQVATEMRERWPEV
                     EGIAIVQRVGHLDPGTPTAVIACSAPHRDSGVFEAARYGIDRLKQIVPIWKKEVGPGG
                     EAWVEGTYQPQSSDQSR"
     gene            complement(1948..2421)
                     /gene="moaC"
                     /locus_tag="VI520_04600"
     CDS             complement(1948..2421)
                     /gene="moaC"
                     /locus_tag="VI520_04600"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HLF37197.1"
                     /translation="MTLSHIDSRGRARMVDVAGKPESERTATARAAVRMSLETLAAAR
                     AGDLKKGDLRAVAELAGVMAAKRTAELIPLCHPLPLTQVAVEIEFSEELPGVEIKASV
                     RTVARTGVEMEALTAVSVAALTVYDMIKGLDRGARITDIRLVEKHGGRSGDYVAG"
     gene            2567..2752
                     /gene="rpmF"
                     /locus_tag="VI520_04605"
     CDS             2567..2752
                     /gene="rpmF"
                     /locus_tag="VI520_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLF37198.1"
                     /translation="MPPLPKRKLSQGRRNRRRAHDALRNRQLLTCTNCGEKHLPHHIC
                     PNCGHYAGREVIEIEHA"
BASE COUNT          523 a          952 c          801 g          501 t
ORIGIN      
        1 tgaatcgaac agggtgatcg gcccaacccc ggacacgccg ttcaccgcgt tttgccagga
       61 ctcggcgacc gagttgccga tcgggttcag cgtccccatt ccggtcacta cgacacgttt
      121 catactgtca agctccattc caggccgact tgtacccgga gccggtcaag accgccacaa
      181 tcggctccgg caaatccggc aagcggcgca gcagtgccga ccaaaccact gccgaagttg
      241 gctctacgaa gaacccacgt tctgcgagcg cccgcctgcc ggccgggatc tcggactctt
      301 cgaccacctc gacgccgccc tgggtcgcat ccaccgccgc taagactgcg tcgcctcgca
      361 gcggattcag gatccggatc ccttcggcca atgtctgccc ctcctgcacc caccccaacc
      421 cggccatccc ggcctcatgc acggcccaga gcggggcgca ggcccgggcc tggacgccga
      481 tgagttgggg aagctggtgg attagcttct ggcgcgccag agcctggaac ccaaggtagg
      541 ctcccaacag cagggtgccc tgccccaccg ggagcagcaa ggcgccgggc gctcggccca
      601 actgctcgac tagctcgaag gcgaaggtcg ccattcctgc caggatctgg ggcatgtagg
      661 cgtggctcgc gtataccgaa cccgcttctg ccgctcgcac gactgcctgg gcggcctccg
      721 agcggggacc taggatggaa acgacctccg caccgtaggc ctcgatctga gcgcgcttgg
      781 gacctgaagc cgagtccggc ataaaaacct tggcccggat tccggcccgt gcggcgtagg
      841 cggcaaagga cgccccggcg ttgccggagg agtcctcgat agcttccctg acttgggctg
      901 ccgccagagc cgagatcagc acggcggtgc ctcggtcctt gaacgatccg gtgggattca
      961 gatactcgca cttaaagaag acccgttttc cctcaatctc ctggccaacc agcggcgtcc
     1021 ccccctcccc aagggagagc agccgcgctc ccggagggag cgggaatgaa tttctgtagc
     1081 gctccaaccc gggcaccggg attggcgcgt agcgcagcgg atcggccagg tcatagtggc
     1141 cgccgcaaac cgcacatcga aacgggagcg cctcccgcgg gtattctcga ccgcagctgc
     1201 tgcagcgaaa aggccaggac caaccatccg gcaactctac cggctctgat cgctggattg
     1261 gggctgatat gtgccttcga cccaggcctc accccccggg ccgacctctt tcttccagat
     1321 cgggacgatc tgtttaagtc gatcgatccc atagcgagcg gcctcgaata ccccgctgtc
     1381 tcgatggggt gcgctgcagg caatgaccgc cgtcggggta cccggatcga gatggcccac
     1441 ccgctgcacg atagcgatgc cttcgacctc tggccagcgc tcacgcatct cggtggccac
     1501 ttggttgagc ttggcttcgg ccatcggcca ataagcttcg tattcgagga agctggtcag
     1561 gcgtggttcg cctcgctcag tttgcgcccg gaccaaccca ctgaagatgc aaaccgcgcc
     1621 ggtcgatgct ccagcaatcg attcgatcag attattcaaa tcgagtggtt cttcggtgac
     1681 cttgagcagg gtcgggtgcg ctccaccgct cactggcggg aacactccca cttcgtctcc
     1741 ctcattcaga ccctcctcgg ggaaggcaaa ctcgtggttc accgaaaaca aggcactgcc
     1801 gagcgccaga gccagtcgat ccccccgctc gctcaagcgg ttcctcaaat cccctactgt
     1861 ggagtgttca gggaggctga gctgctctct gcggagacca gccaaatcgc gcagcgtggc
     1921 aaagtagagg atttcaatct gcacggatca ccctgctacg tagtcgccgg agcgtccgcc
     1981 atgcttctcg accagccgga tgtcggtgat ccgggctccc cggtcgaggc ccttgatcat
     2041 gtcgtaaacc gtcagcgccg ccaccgacac tgcggtcaag gcttccattt ctaccccggt
     2101 ccgcgccacc gttcggacgg aagccttaat ctccacccct ggcagctctt ccgagaactc
     2161 aatttccact gcgacttgag tgagcggtaa gggatgacac agcggaatca gctcggcggt
     2221 tcgcttggcc gccattaccc ccgccagttc cgccaccgcc cgtaggtcac ctttcttgag
     2281 atctccggcg cgggccgcgg cgagagtctc aagcgacatc cggaccgcag ctcgagccgt
     2341 cgcagtccgt tcggattccg gcttaccggc cacgtccacc atgcgcgctc gcccacggct
     2401 gtcgatatgg ctcagggtca cggcccgaat tataccgaat gtgtctttta aactcccggt
     2461 gggtctcgcc gaaagcgtgc gccttgacag cgcgtttggc gacgatataa tgaacccgcc
     2521 tgcccattgg gcaggcgatc ttctgtataa gcacccgagg gattctatgc cgcctctgcc
     2581 gaagcgaaaa ctctcccaag gaagacgcaa ccgccggcga gctcacgatg ccctgcgcaa
     2641 caggcaattg ctcacctgta ccaattgcgg cgagaagcat ttgccccacc atatttgccc
     2701 gaactgcggg cattatgccg gccgggaggt catcgagatc gagcacgcct aacccaatag
     2761 gcgatggcgt ccaactc
//
LOCUS       DATDPQ010000197         4225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1400507, whole genome shotgun sequence.
ACCESSION   DATDPQ010000197 DATDPQ010000000
VERSION     DATDPQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400507"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..694
                     /locus_tag="VI520_04610"
     CDS             29..694
                     /locus_tag="VI520_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37199.1"
                     /translation="MTIASVLLIESGRPSAPSFASALEKKGFAVTVQHEVGPAIKSAS
                     KSPPDVLILDAASMRTSGSRLCRTARANLDGVPIILISPAGTGSESANGSTVNLVQPF
                     TARKLVNRVYRLLPGDDRYSLQAGPIKLNLVQRKVKVHGRESRLTPKQAKLLELFMHN
                     PGKLLTRKQLIREVWLTDYTGDTRTLDVHMSWLRHAVEGNSKKPQFLRTIRGMGYRLD
                     VPT"
     gene            complement(761..2074)
                     /locus_tag="VI520_04615"
     CDS             complement(761..2074)
                     /locus_tag="VI520_04615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HLF37200.1"
                     /translation="MAVGTPLEGPLKNLPTAILQWPGKSGESAAPTPLRQTSLVVPSG
                     GSPVAKPNRLILGDSLGVMTTLLAEFEGQVDLIYADPPFHSGKRYRARTGLAEDSRRP
                     AAWQTTRGFTDTWSGLPEYLSMLYPRLVLMHRLLATTGTLYLHLDWHAAAYGRVLLDE
                     IFGSDRLLNEIIWVYHGPSPIRSGFKRKHDTIYVYTKSKAYTFNADAVRMPYHPTTAK
                     TFASSRKAGFGKRPDLTRGKVPEDWWYFPVVARMHDERTGYPTQKPEALLERIVAASS
                     QPGNLVADFFCGAGTTAVVADRLDRRWLACDISRLAIATTRRRLLLQPVWRSFGVWED
                     PAIPVGPVIEPRMRVRVTGRQVQARLIGVEDSDDEAITGFEVDWDYDDRFFRSRSQSV
                     RRWRSDEFIPVLSNRYRRQGRYTLAAKVMTAQGGVGWVAKDIQLS"
     gene            complement(2052..3383)
                     /locus_tag="VI520_04620"
     CDS             complement(2052..3383)
                     /locus_tag="VI520_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HLF37201.1"
                     /translation="MQPARVIIMGAAGRDFHNFNVYFRDNPAYQVVAFTATQIPNIEG
                     RTYPASLAGELYPEGIPIHPEQQLVELIHAHSADQVVFAYSDVTHEYVMHLASEVLIN
                     GADFRLMGTGTTTLQSSKPVVSVCAVRTGSGKSQTSRRVASLLRQFGHLVGVVRHPMP
                     YGDLTRQAVQRFSEYADLDRHECSIEEREEYEPHIAVGAIVYAGIDYRAVLSAAESEV
                     DIIVWDGGNNDLPFFRSDLHIVVVDPHRPGHELNYHPGEANVRSADILIINKMDTADP
                     ANVEIVRRNIKTLNPNARVIEAASPIQVEDPDAIRDRRVLVVEDGPTLTHGGMSYGAG
                     WIAAQRFKAREIVDPRPYARGSISATYAQYPTTGKILPAMGYGQAQIRELEQTINAAE
                     ADLVLIGTPVNLSRLMSLNKPAQRVRYELEEIGPPQLPSILEQRFGRRNPA"
     gene            complement(3416..>4225)
                     /gene="argS"
                     /locus_tag="VI520_04625"
     CDS             complement(3416..>4225)
                     /gene="argS"
                     /locus_tag="VI520_04625"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLF37202.1"
                     /translation="LRSDGTTLYSAKDLALAKRKFKEYDVDRSIYVVDVRQSLHFQQV
                     FKILELWGFPQAAQCFHLPYGFVTLPEGAMSSRHGHVVLFMDVYNEARRRVEQIIAEK
                     NPELDGERRLEVARQIGLGALTYALISVDNIKDIVFNWDSALSFEGQAAPYIQNAHVR
                     ANSILRKAGSLPESAAFDFELAPLEAELIDWISRFPETVLRAAAEYKPLHVANYAYEL
                     AKTFHAFYHAIPVLQAESTAIQAARLRLTAAARQALANSLHLLGIAAPAVM"
BASE COUNT          841 a         1252 c         1291 g          841 t
ORIGIN      
        1 gttcttttag ctggggggcc ccacagggat gacaatcgcc agcgtcctgc tgattgagag
       61 cggtcggccg agcgccccct ccttcgcgtc agcactcgaa aagaaggggt tcgcagtcac
      121 ggtccagcat gaagtgggac cagcaatcaa gtcggcttcg aagagccctc cggatgtact
      181 cattctcgac gcagcctcga tgcggaccag cgggagccga ctgtgccgga ccgccagagc
      241 caatctggat ggcgtaccga tcatcctgat ttcgcctgcg gggaccggat cggaatcggc
      301 gaacggttca accgtaaacc tggttcagcc gttcacggct cgcaagctgg tcaaccgcgt
      361 atatcggctt ttgccgggcg acgaccgcta ttcgctccag gccggaccga tcaagctcaa
      421 cctcgttcaa cgtaaagtca aagtgcatgg gagagaaagt cgcctaactc caaagcaagc
      481 caagctgctc gaactcttca tgcataatcc gggaaaactg ctcactcgca agcagctgat
      541 tcgggaggtc tggctcaccg actacaccgg cgacacccgg actttagatg tccatatgag
      601 ctggttgcgg cacgcggtgg agggaaattc gaagaagccg cagttcctgc gcaccatccg
      661 cggcatgggc taccgcctcg acgtcccaac ttagaatcag cgaccggaaa ttgccgactc
      721 cggcccatcc gactggatga gcggcccacg ggtaggcgga ttagctgagc tggatatcct
      781 tggccaccca gccgaccccg ccttgggcgg tcatgacttt ggcagccagt gtgtagcggc
      841 cctgccggcg gtagcgattg gacaggacgg ggatgaactc gtccgagcgc caacgacgga
      901 ccgattggga tcggctccga aagaagcgat catcgtagtc ccagtccacc tcgaatccgg
      961 ttatcgcttc atcgtctgaa tcttcaaccc cgatcaatct tgcctgcacc tgccggccgg
     1021 tgacccgaac ccgcatccgg ggctctatta ccggtccgac cggaattgcc ggatcttccc
     1081 aaaccccgaa agatcgccag acgggctgca acagcaagcg gcggcgggtg gttgcgattg
     1141 ccaagcgaga gatgtcgcag gccagccaac gccgatccag ccggtccgcc accaccgcgg
     1201 tcgttccggc gccgcagaag aaatctgcga cgaggttgcc aggctgactc gaagccgcga
     1261 cgattcgctc gagcagcgcc tccggcttct gggtcggata gccggtccgt tcatcgtgca
     1321 tccgagcaac gaccgggaag taccaccagt cttccggcac cttaccgcgg gtcagatccg
     1381 gacgcttgcc aaacccggcc ttgcgggagc tcgcaaaggt cttggcagtt gttgggtggt
     1441 agggcatccg cactgcatcg gcattgaagg tgtacgcctt cgacttcgta tagacataaa
     1501 tcgtatcgtg ctttcgcttg aatccgctgc gaatcggcga tggaccgtgg tagacccaaa
     1561 tgatttcgtt gagcaaccga tcggatccga aaatctcgtc cagcagcact ctgccgtagg
     1621 cagctgcgtg ccaatcgaga tgcagataga gggtcccagt cgtggccagt aagcggtgca
     1681 tcaggaccaa acgcgggtag agcatactca gatactcagg caatccagac caggtatctg
     1741 tgaatcctct tgtcgtctgc caggcagctg gccggcggga gtcctcggcc aggcctgtgc
     1801 gggcgcggta gcgcttgccg gagtggaagg gcggatcggc gtagatcaga tcgacctgcc
     1861 cttcaaattc ggccagcagg gtcgtcatca caccgaggct gtcccccaag atcaaccggt
     1921 tgggtttagc gaccgggctg cccccgctag gcacgaccag gctagtttgc cgtaatgggg
     1981 tgggtgctgc cgactcaccc gatttccccg gccactggag gatggctgtg ggcagattct
     2041 tcaggggtcc ctcaagcggg gttccgacgg ccaaaccgtt gctcaaggat cgaagggagc
     2101 tggggggggc cgatctcttc gagttcataa cgcactcgct gggcaggctt attcaaagac
     2161 atcagccggc tgagattgac gggtgtccca atgagaacca gatcggcttc tgccgcattg
     2221 atggtctgtt ccaattcacg aatctgagcc tggccatagc ccatggccgg aagaattttt
     2281 ccggtcgtgg ggtactgcgc ataggtcgcc gagatgctgc cgcgagcgta cggacgggga
     2341 tcgacaattt cccgggcctt gaagcgctgg gccgcaatcc agccggctcc gtaggacatg
     2401 ccgccgtgag tcaacgtggg accgtcctcc accaccaaca cccgccgatc gcggatggca
     2461 tccgggtcct cgacctggat tggggaagcg gcttcaatca ccctggcatt cgggttcagg
     2521 gtcttgatat tccgccggac aatttctacg ttggcgggat cggccgtgtc catcttgttg
     2581 atgatcagga tgtcggccga gcgcacgttt gcctcgccgg gatggtagtt gagttcgtgc
     2641 ccgggtcggt gggggtcgac gaccacgatg tgcaaatcgc ttcgaaagaa cggcaggtcg
     2701 ttgtttcccc cgtcccacac gatgatatcg acttcggact cggccgcact caacaccgcg
     2761 cggtagtcaa ttccggcata cacgatcgcc cccacggcga tgtgcggttc gtactcctct
     2821 cgctcctcga tgctgcactc gtggcggtcc aaatctgcat actcggagaa gcgttggacc
     2881 gcctgacggg tcaggtcccc gtagggcatt ggatggcgta ccacgccgac aagatggccg
     2941 aattggcgaa gcaggctcgc cacccggcga ctggtctggc tcttgccgct gccggtcctg
     3001 actgcacaga ctgaaacgac cggcttgctg gactgcagcg tggtggttcc ggtccccatg
     3061 agccgaaagt cggcaccgtt gatcaagact tcggaggcaa gatgcatcac atactcatgg
     3121 gtgacgtcgc tgtaggcaaa cacaacttgg tccgccgaat gggcatggat cagttcgacc
     3181 aattgctgtt cggggtggat ggggattcct tcggggtata gctcaccggc cagtgaggcc
     3241 ggataggtcc ggccttcaat attcgggatt tgagtcgcag tgaaggcgac cacctgatag
     3301 gcgggattgt cgcgaaaata gacattgaag ttatggaaat ctcggccggc ggcacccatg
     3361 ataatcactc gagctggttg catggtcggt ttcggcggct cctccccgct ggcaatcaca
     3421 tcacggcagg cgctgcaatg cccagcaagt gcaagctgtt ggcaagcgcc tgtcttgcgg
     3481 cggcggtcaa cctaagccgg gctgcttgga ttgcggttga ttccgcttgg agcacgggaa
     3541 tcgcgtggta gaaggcgtgg aaggtcttgg ccaactcata ggcgtagttg gcaacgtgca
     3601 gcggcttgta ctcggcggcc gctctgagga ctgtctccgg aaagcgtgag atccaatcaa
     3661 tcagctcggc ctcgagcggt gcgagctcaa aatcgaaagc ggctgactcc gggagacttc
     3721 cggccttcct caagatgctg ttggcccgga catgggcgtt ttgaatgtag ggagcggcct
     3781 gcccctcgaa actcagggcc gagtcccaat tgaatacgat gtccttgata ttgtctaccg
     3841 agatcagggc gtaggtgagg gcgccgagcc caatctggcg agccacttcc agcctccgct
     3901 ctccatccag ttcggggttc ttctcggcaa tgatttgttc caccctccgc ctggcctcgt
     3961 tatagacgtc cataaaaagg actacgtgtc catgccgcga cgacatggct ccctccggga
     4021 gcgtgacgaa cccgtagggc agatggaagc attgggcggc ctggggaaac ccccaaagct
     4081 cgaggatctt gaatacctgc tgaaagtgca gactctgtct gacgtctacc acatagatcg
     4141 agcggtccac gtcgtattcc ttgaacttgc gtttcgccag cgccaggtcc ttcgccgagt
     4201 agagggtcgt cccgtcgctg cgcag
//
LOCUS       DATDPQ010000198         4228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3237535, whole genome shotgun sequence.
ACCESSION   DATDPQ010000198 DATDPQ010000000
VERSION     DATDPQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237535"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VI520_04630"
     CDS             <1..666
                     /locus_tag="VI520_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37203.1"
                     /translation="SLDGLVEGARAANAQGNWDAAIEILVTLLGESPTHRRPEVNQLL
                     YTALRNRGLDKIFRGEREQGIYDLALAERITALDGQAQSWWTSAAFYSFANSYFGLDW
                     GLSTQYFSQICAAGIWDGCFKYARSAWEYADLLFKDLDSCGSVIQYDASLRTADREDG
                     PPTATQAFTICLTATAVTPTPSPTIDLSVTPTGTAGPSPTPTESPTATPTFTETPTET
                     PSS"
     gene            740..2191
                     /gene="sthA"
                     /locus_tag="VI520_04635"
     CDS             740..2191
                     /gene="sthA"
                     /locus_tag="VI520_04635"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HLF37204.1"
                     /translation="MSAYDYDLLVIGSGPAGQRAAIQAAKLGKRVGLVERKTVVGGVC
                     INTGTIPSKTLREAVLYLSGYRLRGIYGVSYAVKQDITMADLLFRTDNVISHELDVIR
                     HQLTRNGVVLLNAEASFIDPHTLRLRSAFDTAERQVTADKVVIATGTNATRSPDIAFD
                     GRWILASDDILTLDQIPRSMAVVGAGVVGCEYASIFAALGVRVTLIDKRQRLLEFIDS
                     EIAETLIYHLRQNRVTIRLGEEVSAIESMQTELGEQVRIHLTSGKKLTAEKALYSVGR
                     MGATEALNLEAIGIQPDVRGRLKVNPHYQTELPHVYAAGDVIGFPSLASTSLEQGRLA
                     ACHAFGLQAKSVPELFPYGIYTIPEVSFVGKNEQELTEQGVPYEIGKAQYRESARGQI
                     IGDNSGLLKLIFHLGTRELLGVHIIGEGASELIHIGQAVLAFGGTVDYFVDTVFNYPT
                     LAECYKTAAFDGINRLGSPQDTISIRPMPKRDP"
     gene            2218..3447
                     /locus_tag="VI520_04640"
     CDS             2218..3447
                     /locus_tag="VI520_04640"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLF37205.1"
                     /translation="MTKQLLFSELAAGIQESATIRLNQQAARLRAEGKPMIHLGIGEP
                     TNRAPQTAIEAAEKLLAAGSLKYTATAGVPSLRQAIADFMLASYGREVPPANVIASNG
                     SKHSLLTVLLATVNPGEQVIIPAPYWVTYPEQVKLVRGRPIIVLPEPGSLLPSAESLL
                     AAVGPLTRAILLNSPNNPSGLVLPESDLATVVEFCEARGIYLILDDIYRELVFDGLRL
                     PSPYAFTRFELDESHVIVVNGVSKLYGMTGLRLGWTVGPRALVEAMDKLQSQMTSNPS
                     ILSQAAAEGALRGDQRVVQELRLTVEANRDAMLEALGEMAAMQVARPQGGLFCFPDFS
                     AHGSSSKELAERLLERALVVTVPGVEFGMDGHLRLSFAGDREEILEGVRRIRWALDPA
                     SPNEIRLGDRVAVRDWA"
     gene            3476..>4228
                     /locus_tag="VI520_04645"
     CDS             3476..>4228
                     /locus_tag="VI520_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLF37206.1"
                     /translation="MDWIADPETWIALLTLTALEIVLGIDNIVFISILAGKLPGPDQD
                     RARQTGLLAAMGTRIGLLFSLSWLIGLTEPLFAAFGMEISGRSLILIAGGLFLLAKST
                     LEIHDKLEGKAGASSARVIPSFRGVVIQIMLLDIVFSLDSVLTAVGMAKQLAVMVAAV
                     VIAVLIMLAASGPISRFVAERPTVKILALSFLLMIGVSLIAEGLDFPIPKGYIYFAMA
                     FSVFVETLNLRLRAREARPIQLRQAYVAGNPSG"
BASE COUNT          774 a         1319 c         1348 g          787 t
ORIGIN      
        1 tcgctggatg ggctggtcga gggggcgcgg gcggccaacg cccaaggcaa ctgggacgcg
       61 gccattgaga tcctggtcac cctgctcggc gagtctccga cccatcgccg tccggaagta
      121 aaccagctgc tctacaccgc actgcggaat cgcgggttgg acaagatctt ccgcggcgaa
      181 cgggagcagg gaatctatga cctggcgctc gccgagcgga tcaccgccct cgacggtcag
      241 gcccagagct ggtggacttc ggccgccttc tattcattcg ccaacagcta tttcggattg
      301 gattgggggc tctccaccca atacttctcc cagatctgcg cggccggaat ctgggacggc
      361 tgcttcaaat atgcacgctc ggcctgggaa tatgccgact tgctgttcaa agatctagat
      421 tcgtgcggct cggtaatcca atacgatgcc tcgctccgca ccgcggatcg ggaagacgga
      481 ccgcccaccg cgacccaggc tttcaccatc tgtctgaccg ccactgcggt aactcccact
      541 cccagcccga cgatcgacct ctccgtcacc ccgaccggta cggccgggcc gagtccgacg
      601 ccgaccgaat cgccaacggc gactccgact ttcaccgaga ccccgaccga gacaccgagc
      661 tcgtagcggc gcagtagaat aggcctcgac ctgcgggcgg gattgaagct cgggcagaca
      721 tgtcaggaac tccaaaagag tgagcgccta cgattatgac ctgttggtga tcggatccgg
      781 cccggccggc cagcgggccg cgatccaggc agccaaactc ggtaaacggg tagggctggt
      841 cgagcgtaag acggttgtcg gcggggtgtg tatcaacacc ggcaccatcc ccagcaagac
      901 gctccgggaa gcggtgctct atctctcggg ttaccgctta cgcggaatct acggcgtttc
      961 ctacgcggtc aagcaagaca ttacgatggc cgacctgctg ttccgcaccg ataacgtcat
     1021 cagccatgag ctggatgtca tccgtcatca gctgacccgc aacggggtgg ttctgctcaa
     1081 cgcagaagca tctttcatcg atccccacac gctgaggctg cgatccgcgt ttgacaccgc
     1141 cgagcggcag gtgaccgccg acaaggtcgt gatcgccacc gggaccaacg ccacccgcag
     1201 ccccgacatc gccttcgacg ggcgctggat cctggcgagc gacgacatcc tcacattgga
     1261 tcagattccg cgtagcatgg cggtggtcgg agccggtgtg gtcggctgtg agtacgcttc
     1321 gatcttcgct gccctgggag tgcgggtgac cctgatcgat aagcggcagc gcctgctcga
     1381 gtttatcgac tcggagatcg cggagacctt gatctaccat cttcgccaga accgggtgac
     1441 catccgactg ggcgaggagg tcagcgccat cgagtcgatg cagaccgagc ttggggagca
     1501 agtccggatc cacctcacca gcgggaagaa gctgaccgcc gagaaggcgc tctacagcgt
     1561 cggcaggatg ggggccaccg aggcgctcaa cctggaggcc atcggcatcc agccggacgt
     1621 gcgtggccgg ctcaaggtca acccgcacta tcagaccgaa ctgccgcacg tgtacgcggc
     1681 aggggacgtg atcggattcc cgagcctggc ctccacttcg ctcgagcagg gccggctggc
     1741 cgcctgccat gcctttgggc tgcaggccaa gagcgttcca gaactcttcc cctacgggat
     1801 ctacaccatt ccggaggtct cgtttgtcgg gaaaaatgag caggagctga ccgagcaagg
     1861 agtcccatac gaaatcggga aggcacagta ccgcgagagt gcccgcgggc agatcatcgg
     1921 agacaacagc gggctgctta agctgatttt ccacctcggg acccgcgagc ttttgggcgt
     1981 gcacatcatc ggggaagggg cgagcgagct gatccacatc ggccaggcgg tgctggcctt
     2041 cggtggcacc gtagattact tcgtcgatac ggtcttcaac tatccgaccc tggccgagtg
     2101 ctacaagacc gccgcattcg acggtatcaa ccggctgggt tcgccgcagg ataccatctc
     2161 gatccgaccg atgccgaaac gcgaccccta gccgccggat ccttgattct gagtgccatg
     2221 accaagcaat tgctcttcag cgaactggcg gccgggatcc aggagtcggc caccatccgg
     2281 ctcaaccaac aggccgcccg gctgcgggcg gagggaaagc cgatgatcca cctcgggatc
     2341 ggcgaaccga ccaatcgcgc tccccaaacc gcgatcgagg cggcggaaaa gctgcttgcc
     2401 gccggatccc tgaagtacac cgctaccgcc ggagtcccct cgctgcgcca ggcgatcgcg
     2461 gatttcatgc tggcctctta tggccgggaa gtccctccgg ccaatgtaat cgcctccaac
     2521 ggatcgaagc attcgctgct cacggtcttg cttgctacgg tcaacccggg cgagcaggtc
     2581 atcatcccgg ccccgtactg ggttacctat ccggaacagg tgaaactggt gcgcggtcgg
     2641 ccgatcatcg tactgccgga gccggggagt ttgctcccca gcgccgaatc tttgctcgcg
     2701 gcggtgggcc cgctgacccg ggcgattctg ctcaacagtc cgaacaaccc ttcggggctg
     2761 gtgctgcccg agtccgatct ggccacggtc gtcgagttct gcgaggcccg cggcatctac
     2821 ctcattctgg acgacatcta tcgcgagttg gtgttcgacg gactgcgatt gccatcgccc
     2881 tacgcgttca ctcgattcga gctcgatgag agccatgtca ttgtggtgaa cggggtttcg
     2941 aagctctacg ggatgaccgg actacggctg ggctggacgg tcggcccgcg ggcgctggtg
     3001 gaggcgatgg acaagctgca gagccaaatg acctcgaatc catccatttt gtcccaggct
     3061 gcggccgaag gggcgctgcg cggcgaccag cgggtggtgc aggaactgcg attaacggtc
     3121 gaggccaatc gggatgcgat gctggaggcc ctgggcgaga tggccgccat gcaagtcgcc
     3181 cgcccacaag ggggcctgtt ctgctttcca gatttttcgg ctcacggctc gagctcgaag
     3241 gagctggcgg agcggctgct agaacgggcg ctggtggtga ccgtgccggg ggtcgagttt
     3301 ggaatggacg gccatttgcg gttgagcttt gccggggatc gggaggaaat cctagagggg
     3361 gtacggcgga ttcgctgggc cctcgatcca gcctccccga atgaaatccg attgggtgac
     3421 cgggtggccg tgcgggactg ggcctagcgg ccaaccgaga ataggaatca tagcgatgga
     3481 ctggatcgcc gaccccgaaa cctggattgc gctgctgacc ctcaccgcgc tggagatcgt
     3541 gctcggaatc gacaacatcg ttttcatttc catcttggcc ggcaagcttc ccggacccga
     3601 tcaggaccgg gcccgccaga ccggcttgct ggcggctatg ggaactcgaa tcgggttgct
     3661 attttcgctg agctggctga tcgggcttac cgagccgctg ttcgcggcct tcggcatgga
     3721 gatatccggc cgttcgctga tcctgatagc cggcgggttg ttcctgcttg caaagagtac
     3781 tcttgagatc cacgacaagc tggaaggcaa ggccggggcg agttccgccc gggtcatccc
     3841 gtcgttccgc ggggtcgtga tccagatcat gctcctggac atcgtgttct ccctcgattc
     3901 ggtgctcacc gcggttggga tggcgaagca gctggcggtg atggtggcag ccgtggtgat
     3961 cgcggtgctg atcatgctgg cggcctccgg accgatcagc cgatttgtgg ccgagcgtcc
     4021 cacggtgaag atcctggcgc tcagctttct gctgatgatc ggggtctcgc tgatcgcgga
     4081 agggctcgat ttccccatcc cgaagggcta tatctacttc gccatggcct tctcggtgtt
     4141 tgtagaaacg ctcaacctgc gcctgcgggc ccgggaggcc cggccgatcc agctgcggca
     4201 ggcctatgtg gccgggaatc cgtctggg
//
LOCUS       DATDPQ010000199         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_869429, whole genome shotgun sequence.
ACCESSION   DATDPQ010000199 DATDPQ010000000
VERSION     DATDPQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869429"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VI520_04650"
     CDS             <1..1041
                     /locus_tag="VI520_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HLF37207.1"
                     /translation="LSMILLLGSTLARPVALQTTVAQAVLFYTPTCPYCHKVMTEDLP
                     PLLETLGGQLEILAADTATARGQALYQAAIDQFQISDERRGVPTLIIGEDILVGAEEI
                     PQQFPGLVQASLNAGGVAFPSLPEFGAYADQFPRFTADGVVGLNSESIGDRVGRDPLG
                     NGLAIGILVGMLLSVYQVVSRVRAYRNRPRSLRKKAEQEDASQLGGWAIPLLAVAGLL
                     ISGYLTAVGAGGAEAFCGPVGDCDAVQESQYSQLFGVISVSVIGLLGFAAILVVWVIA
                     KFGGEQSAAKAGGLLLPLTVGGTLFAIYLTFLEPFVIGAACSWCLISAILITALMWLT
                     ARPALARTRRPA"
     gene            1062..2042
                     /locus_tag="VI520_04655"
     CDS             1062..2042
                     /locus_tag="VI520_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLF37208.1"
                     /translation="MFERILVPLDGSGLAERALHPALRLATAPQHELFLLRVPEAKTM
                     FEPGQSGLFDYGLLWPQQALERNSHEARKYLQSICRQVPDPSLRLRILLSEGDPASVI
                     VDTASQEGIDLIAMSSHGYSGFTRWMMGSVADRVVRAARCPVLIVRSEEPIRNVLITL
                     DGSELSERALEPGIELAARLDCPVTLFRSVTDPAMDEGMARQLDLLEPGLGERLLQHP
                     YRAVETYLERIARDYRRAGSDLKTVAASGSPASRILDHAARYGVDLIVMATHGRSGVN
                     RWVHGSVTEKILRASGCSMLIVPAGFAPRPTVPAHQSVEEHFELARPSLN"
     gene            2054..>2887
                     /locus_tag="VI520_04660"
     CDS             2054..>2887
                     /locus_tag="VI520_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HLF37209.1"
                     /translation="MPDSIATLDGNEAVARVAYLLNEVIAIYPITPASAMGEWADQWA
                     AEGRPNLWGTVPQVIEMQSEGGAAGAVHGALQVGSLTTTFTASQGLLLMIPNLYKIAG
                     ELTPAVFHIAARALAAQALSIFGDHSDVMAARSTGFALLASNSVQEAQDFAAIAQAAT
                     LEARIPFLHFFDGFRTSHEINKIQLLTNDQLASLIDDNLIRAHFERALTPDHPSIRGT
                     AQNPDVYFQARETVNSHYVACPGIVQGAMDRFAALTGRRYQLFEYVGVPDAERVVVLM
                     GS"
BASE COUNT          485 a          907 c          897 g          598 t
ORIGIN      
        1 ttatccatga tcctgttgct gggctcgacc ctggcccgcc cggtggctct ccaaacaacg
       61 gtcgcgcagg cagtcctgtt ctacaccccg acctgcccgt attgtcacaa ggttatgacc
      121 gaggacctcc cgccgctgct agagaccctc ggcggccaac ttgagattct ggccgccgac
      181 acagcgacgg ctcgcggcca agctctctac caggccgcaa tcgaccagtt ccagatttca
      241 gatgagcggc gaggggtccc caccctgatc atcggggaag atatcctggt tggcgccgag
      301 gaaatcccac agcagttccc ggggctggtc caggcgagcc tgaatgcagg gggagtcgcc
      361 tttccttcgc tcccggaatt cggggcgtat gccgatcagt tcccgcgttt cacggcggac
      421 ggtgtggtcg ggctgaatag tgagagcatt ggggaccggg taggccggga tccgctggga
      481 aatgggttgg cgattgggat cctggtgggg atgctgttga gcgtgtatca ggtcgtctcc
      541 cgggtgagag cgtatcggaa tcgaccccgc agcttgcgca agaaagcgga gcaggaggat
      601 gcttcccagc ttggcggctg ggcgatcccg ctgctggccg tcgccgggct gctcatctcc
      661 ggctacttga ccgcggtcgg ggccggcggc gcggaggctt tctgtggacc agtcggcgac
      721 tgcgatgcgg tccaggaaag tcagtactcc caactgtttg gggtcatctc agttagtgtc
      781 atcggcctgc tcggcttcgc cgcgattctt gtcgtctggg tcattgccaa gtttgggggt
      841 gaacaatccg cggccaaggc gggcggccta ctgctcccgc tgaccgtcgg cgggacgttg
      901 tttgctattt atctaacgtt tctggaaccg tttgtgatcg gagcagcctg ttcgtggtgt
      961 ttgatttctg cgatcctcat cacggccctc atgtggttga ccgcgaggcc ggcgttggcc
     1021 cgcactcgcc ggccagcttg atagtgcata ggaggtccac aatgttcgag cgaatcctcg
     1081 tcccactcga tggatccggg ttagccgagc gagcactcca cccagctctg cgcctggcca
     1141 ccgctcccca gcacgaactc ttcctgcttc gggttccgga agctaagacc atgttcgaac
     1201 cgggacagtc cggtctattc gattatgggc tgttgtggcc gcagcaggcg ttggaacgga
     1261 attctcacga ggcgcggaag tacctccagt cgatctgccg gcaagttccc gatccttctc
     1321 tgcggctgcg gattctgtta tccgaaggcg accccgcctc cgtcatcgtc gacactgcct
     1381 ctcaggaagg catcgatctc atcgcgatgt cctcccatgg ctattcaggt ttcactcgct
     1441 ggatgatggg gagtgtggcc gaccgggtag tgcgggccgc ccgctgcccg gtgcttatcg
     1501 tgcgatcgga ggagccgatt cgcaacgttc taatcacgtt ggacggctcc gaattgtccg
     1561 agcgggcgct cgagccgggg atcgagctcg ccgcgaggct ggactgccca gtcactttgt
     1621 ttcgctcggt aactgacccg gcgatggacg aagggatggc gcggcagctt gatttgctcg
     1681 agcctggcct cggtgagcgg ctgctccagc atccctaccg ggcagttgaa acgtacctgg
     1741 aacgcatcgc gcgggattac cgccgtgccg ggtctgatct aaagaccgtg gcagccagcg
     1801 gctcgccggc ctccaggatc ctggatcacg ccgcacgcta tggtgtggat ctcatcgtga
     1861 tggcgaccca tgggagatcc ggcgtgaacc gctgggtaca cggaagcgtt accgagaaaa
     1921 ttctgcgggc ctcgggttgc tcaatgctca tcgtgcccgc gggattcgca ccccgaccaa
     1981 cggtccccgc tcatcaatcg gtcgaggaac atttcgagct cgcccgcccg agtctgaact
     2041 aaggagggcc agcatgcccg attcgatcgc gacccttgac ggcaatgagg ccgtcgcccg
     2101 agtcgcctat ctgctgaacg aagtaatcgc catctatccg atcaccccgg cctccgccat
     2161 gggcgagtgg gcggatcaat gggcggcgga aggccggccg aacttgtggg gaacggttcc
     2221 ccaggtgatc gagatgcagt ctgagggcgg agcggccggc gcagtgcacg gtgcacttca
     2281 ggttggttcg ctcactacca cctttaccgc ctcccagggg ctgctgctca tgattcccaa
     2341 cctgtataag atcgccggcg agctcacccc tgcggtgttc catattgccg cccgtgcgct
     2401 ggccgcccag gcgctttcga tcttcggcga tcacagcgat gtcatggccg ctcgcagcac
     2461 cggctttgcc ctcctggcct ccaattcggt gcaggaggcg caggactttg ccgccatcgc
     2521 ccaggccgca acccttgagg ctcgaattcc attcctgcat ttcttcgacg gcttccgcac
     2581 ctcgcacgag atcaacaaga tccagctatt gaccaacgac cagctggcga gtctcatcga
     2641 cgataacctg atccgtgctc atttcgaacg ggccctcacg ccggatcatc cctcgatccg
     2701 cggcaccgcc cagaacccgg acgtctattt ccaggcccga gagacggtca attctcatta
     2761 cgtagcctgt ccggggatcg tccaaggggc gatggatcgc ttcgcagccc tgaccgggcg
     2821 ccgataccag ctcttcgagt atgtcggagt cccggacgcc gagcgggtcg tggtgctcat
     2881 gggctct
//
LOCUS       DATDPQ010000200         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1401060, whole genome shotgun sequence.
ACCESSION   DATDPQ010000200 DATDPQ010000000
VERSION     DATDPQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401060"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /gene="glp"
                     /locus_tag="VI520_04665"
     CDS             complement(<1..1169)
                     /gene="glp"
                     /locus_tag="VI520_04665"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HLF37210.1"
                     /translation="MSGLTKLVPVDQALRSFLAAIPDPDPGSTELIRTEDALGRALAG
                     PVIAPHSLPPFDRTTVDGYAVRAADTLGASSTHPANFSLAGEVLMGTAPQLGVDHGQA
                     ALVHTGGMIPLGADAVVMIEDTQAVNGSDIVVLKAVASGQNVIREGEDVAAGQVVIPA
                     GVRLRPQEIGGLMALGIVQVQVRLRPRVALLSTGDEVVNPATQPAPGQIRDVNSYSLG
                     TLVEQAGGRPIRYGIIRDSAAELEAVSRRAQAESDLIAIAAGSSVSVRDLTAGVIRTL
                     GPPGILVHGVSMRPGKPTILAVCNQVPVVGLPGNPVSALVVAGLFLLPALRKLLGLRG
                     PEWPSVRQARLTSKVTSAIGREDYLPVRLEVSPQGLLAAPVHGGSNMIFTLVRADG"
     gene            complement(1172..2086)
                     /locus_tag="VI520_04670"
     CDS             complement(1172..2086)
                     /locus_tag="VI520_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HLF37211.1"
                     /translation="MPAWRDLRLFLRLTRPHFLLGGVLLYGLGAAIAHYLGHPIGPGP
                     YALGQALVTLLQLMTHYLNEYYDAPADQQNQARTLLTGGSGVIGPGALSAKVALYSAV
                     ISLGLAAVIASASLVTGSLPFLAWPVLLLGFLGGFFYSVPPLRLAASGYGELVASLVV
                     GALVPLFGYSVQVGETHRLLFMSVTPLVGLCFAMLIVFELPDYPTDLKFQKRTLVVRL
                     GWLAAMRAHDVALGFGLLSFGVIFLNGVPARVAASALFLIPLVIAQVWQLRRIKAGYP
                     PKWGSLTFGSLAMFAIGAYLELAGYVLS"
     gene            complement(2088..>2500)
                     /locus_tag="VI520_04675"
     CDS             complement(2088..>2500)
                     /locus_tag="VI520_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37212.1"
                     /translation="ARRRIMHDPLPIHDLYIRPLDLRTTPAGSTLTVLRYSDHLLRRF
                     GFAEFHELAAGQQRASEARPVADEVWALVRGEVEFDWEDRREDSPTLDRTYHLHANRP
                     TVVLVPFGVAFRIRSLSGGAALVRLASHEDGDSS"
BASE COUNT          516 a          800 c          800 g          384 t
ORIGIN      
        1 ccatcggccc tcacaagagt aaagatcata ttggatccgc catggactgg cgccgccagc
       61 agcccctgcg gcgagacttc cagccgcacc ggcaggtaat cttcgcggcc aatggccgag
      121 gtcaccttcg aggtcaggcg ggcctgcctc acggagggcc attcgggtcc gcgcaacccg
      181 agcaattttc gcaaggccgg cagcaggaac agtccggcca ccaccaacgc actgacggga
      241 tttcccggca ggccgacaac tggcacttgg ttgcatacgg cgaggatcgt cggtttgccg
      301 ggccgcattg agaccccgtg gaccaggatc ccgggcgggc ccaaagttcg gattaccccg
      361 gcggtgagat cgcgcacgct caccgaggac ccggcagcaa tcgcgatcaa atcggactcg
      421 gcctgagctc gccgggaaac ggcctcaagt tcggccgctg aatctcggat gatcccatag
      481 cggatcggtc ggccaccggc ctgttccacc agcgtaccca gcgaatagct gttcacatcc
      541 cgaatctgcc caggggccgg ctgggtcgcc gggttgacca cctcatcgcc ggtcgacaag
      601 agtgcaaccc tgggccgaag tcggacttgg acctggacga tcccgagtgc catcagtccg
      661 ccgatctcct gagggcgtag gcgcacccct gccgggataa cgacctgacc tgcggccacg
      721 tcttcgcctt cccggatcac gttttgcccg gaggcgaccg ccttcagaac cacaatatcg
      781 gacccgttca ctgcctgggt gtcctcgatc atcaccaccg catccgcgcc gagggggatc
      841 attccgccag tgtgaaccag cgcggcttgg ccgtggtcga ctccgagctg cggcgcggtc
      901 cccatcagca cctctccggc caagctgaaa ttggcagggt gggtcgagga ggcccccaac
      961 gtatcggcag cgcggaccgc gtacccgtct accgtcgttc ggtcaaatgg aggcagcgaa
     1021 tggggagcaa tcaccgggcc agccaaggcc ctgccgagcg catcttcggt ccggatcagc
     1081 tcggtcgaac ccggatcggg atccgggatc gctgccagga aggagcgcag cgcctggtct
     1141 acggggacga gtttggtgag gccggacatt gttaactgag cacgtagcct gccaattcca
     1201 ggtacgctcc aatcgcaaac atagcgagtg agccaaaagt caaactgccc catttcgggg
     1261 ggtagcccgc cttaattcgg cgcagctgcc agacctgtgc gatgactaga gggatcagaa
     1321 acagagcact ggccgctacc cgagctggga ccccgttaag gaaaattacg ccaaagctca
     1381 acaggccaaa gcccagcgcg acatcgtgtg cccgcatggc cgccaaccag cccagccgga
     1441 cgaccagcgt gcgtttctgg aatttcaagt cggtagggta atcggggagc tcgaacacga
     1501 tcaacatggc aaagcagagc ccgaccagcg gcgtgacgct catgaatagc agccggtgag
     1561 tttcaccaac ctggacgctg tatccgaata gtggaaccag cgcgccgacg acgagcgagg
     1621 ccaccaactc gccgtagccg ctggcggcca gccgcagcgg cgggacgctg tagaagaatc
     1681 caccgaggaa cccgagcagc agcaccggcc aggccaagaa gggcagagat ccggtgacca
     1741 gactggccga ggcaatcacc gcggcaaggc ccagtgagat cacggcagaa tacaacgcca
     1801 ctttggcgga cagcgctccc ggtccgatca ccccactgcc tccggtgagc agagtgcggg
     1861 cttgattctg ctggtccgcc ggggcgtcgt agtactcgtt gaggtaatgg gtcatgagct
     1921 gcaagagcgt gacgagggcc tgcccgaggg cgtacgggcc cggaccgatg gggtgaccca
     1981 agtaatgggc gatcgccgcc cccaggccgt agagcagcac gcctccgagc aggaagtgcg
     2041 gacgggtcag gcggagaaac agccgtaaat cgcgccaggc cggcatgtca ggagctgtct
     2101 ccatcctcgt ggctggcaag gcgtacgagc gccgctccgc cgctcagcga tcgtatccga
     2161 aatgcaaccc cgaacggaac gaggaccaca gtcggtcggt tggcgtggag atgataggtc
     2221 cggtcgaggg tcggtgaatc ttcgcgccgg tcctcccagt cgaactccac ttcgccgcgg
     2281 acgagcgccc agacttcatc ggcgaccggt cgggcttcgg atgctcgctg ttggcccgcc
     2341 gccagctcgt ggaactccgc aaatccaaag cgtcgaagca gatgatccga gtaacgcaga
     2401 actgtgagag tagagccggc gggagttgtc cgcaggtcaa gcggacggat gtacaggtca
     2461 tggatcggga gcggatcgtg cattatccgc cggcgagcgc
//
LOCUS       DATDPQ010000201         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_602294, whole genome shotgun sequence.
ACCESSION   DATDPQ010000201 DATDPQ010000000
VERSION     DATDPQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602294"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VI520_04680"
     CDS             <1..270
                     /locus_tag="VI520_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37213.1"
                     /translation="LDCETAPSGDPRIWCLSGAVQNTFWDPTGQDQALDFCARLEVEA
                     EKAGCYETIFNRAPLVLASEEDLQAFCSKAELDYQEACLTAVANS"
     gene            379..2157
                     /locus_tag="VI520_04685"
     CDS             379..2157
                     /locus_tag="VI520_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37214.1"
                     /translation="MRSTLERHGLLVGLAVLLWLVAVGRFGAYGFSWDTFILWFGALV
                     GVAQLARLADGDLRAKVANLTQELAPLAVLLAVFSPLYLLFLYHLPQQVNTDEISLMI
                     FERLNLTRPVNLLGVSPYFSFPSLPFILFSRLGVLLGGINLSNMRLVHAGFGLLIIFL
                     GYALFRLHMPRGQALGGAVLLGSNHALLGISRLAQWDNLSLFAELAALIVLVAALRRG
                     SHFIAVLGGFLAGLSFYVYLPGRITIVIWLLYLGVCWLLRRDTQGRRLILRVGVASSI
                     SFLMVATPITVGQLKDTRPGHNYYKRQLMIFPEGRAYQQQWFRAQTVAEGIWINISHG
                     LTAFNNQEYDRGFIYENRGHGFLDPLTGVLLWVGVFYAARELSRKDQAQDGNALALTG
                     FGALWFTYTLVTNQTPNYTRMLVVLPFVAYLATEGLRRVAAIPLIPRIALPKSLGWIR
                     GYEWFAIGVFVVAAWNAVIYSKYIEFGFTQGNMIGATARLVAHRSTNPDLELYLFADE
                     NLPYYTWGGPEQWKEWAGTFAQEGQILQVADGEECRYSELFAPFIAFMSAQVWELCRP
                     TLLTAFPTLQVENMLPNGTRLAVEVP"
     gene            complement(2192..>2731)
                     /locus_tag="VI520_04690"
     CDS             complement(2192..>2731)
                     /locus_tag="VI520_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37215.1"
                     /translation="RVPAGGGAPDQVIENAEQPSFSADGSRLAFFRFNVETYTRGLWI
                     ANGDGTDAREVVSDTTFPDLLSPRLSPDGATIAFAAAGPPVGPPPTSRSLWDWLFGVE
                     VAYAHGLLPSDFWTVPSAGGQPTQITQWGTDGTVLDWSPDGDRLALMHLGGLFVTTVG
                     EPAFLTETPNHGGIDWSEG"
BASE COUNT          493 a          832 c          827 g          579 t
ORIGIN      
        1 ctggactgtg aaacggcgcc ttccggcgat ccccggatct ggtgtttgag cggggcggtt
       61 cagaacacct tctgggatcc aaccgggcaa gatcaggccc tcgatttctg cgcccgcctg
      121 gaagtcgagg cggaaaaagc cggctgctac gaaaccatct tcaaccgggc gcccttggtg
      181 ctggctagtg aggaagatct gcaagccttc tgcagcaagg ccgaattaga ctaccaggag
      241 gcctgcctga cggcagtagc aaattcctag gccttgcggc agtcgagtgt gagatcatcg
      301 aaagctgccc cacgattcgg cgccgcctag attatgcaaa tcgctagttt tccccgcctg
      361 aggtcgaaga aggatggctt gcgctcaacc ctcgagcgcc atgggctgct ggttgggctg
      421 gcggtcctgt tgtggctggt cgcggttggt cgattcgggg cctacgggtt cagctgggac
      481 accttcatcc tctggttcgg agccctcgtc ggagtcgccc aactcgcccg gcttgcggac
      541 ggagacctcc gagcaaaagt cgccaatctg actcaggaat tagccccgct ggcggtactc
      601 ctggcggttt tttccccgct ctacctgctc ttcttgtatc acctgcccca gcaagtcaat
      661 accgacgaga tctcgctcat gatcttcgag agattgaatc tcactcggcc ggtcaatttg
      721 ctgggggtca gcccatactt cagctttccc tcgcttccgt tcattctgtt cagccggctt
      781 ggggtgctgc ttggggggat taatctgagc aacatgcggc tggtgcatgc gggatttggt
      841 ctgctgatta tcttccttgg atacgcactc ttccggctcc atatgcctcg cggccaggcc
      901 ctgggcggag cggttctgct cggttcaaac catgccctgt tggggatcag ccggctagcc
      961 cagtgggaca acctcagcct gtttgcggaa ttggccgcac tgattgtatt ggtcgccgcg
     1021 ctgcgccgcg gctcgcactt cattgcagtc ctgggtgggt ttctggccgg gctctccttc
     1081 tacgtgtatc tgccgggcag gatcacgatc gtgatctggc tcctgtatct gggtgtgtgc
     1141 tggctgctgc gccgcgatac ccagggtaga cggttgatcc tgagagtagg tgtggcgagc
     1201 tcgatcagct ttctgatggt cgcgaccccg atcacggtcg gacagcttaa ggacacccgt
     1261 ccgggacaca actactacaa gaggcagctg atgatcttcc ccgagggtcg ggcttaccag
     1321 cagcagtggt tccgagccca gaccgttgcc gagggcatct ggatcaacat ctcccatggg
     1381 ctcaccgcct tcaacaatca agagtacgac cgggggttca tctatgaaaa ccgcgggcat
     1441 ggttttcttg acccgctgac cggggtgctt ctgtgggtcg gagttttcta tgctgcccgg
     1501 gagctgtccc gcaaggacca ggcccaggac ggaaatgcac tggcgctcac cggatttggg
     1561 gccctgtggt tcacctatac cttagtgacg aaccagactc cgaactacac ccggatgctg
     1621 gtggtgctgc cattcgtcgc ctatctggca acggaggggc tgcggcgagt agcggcgatt
     1681 ccgctaatcc ccaggatcgc gctccccaaa tcgcttggct ggatccgcgg ctacgagtgg
     1741 tttgctatcg gggtgttcgt ggtcgcggcg tggaacgcag tgatctactc aaagtacatt
     1801 gaattcggct tcacccaggg gaatatgatt ggcgcgaccg cacggctcgt cgcccaccgg
     1861 tcgacaaatc cagaccttga actctatctc ttcgcagatg aaaacctgcc atattacacc
     1921 tggggaggcc ctgagcagtg gaaggagtgg gccggcactt ttgcccaaga gggccagatc
     1981 ttacaagtag cggatggcga ggagtgtagg tattccgagt tattcgctcc gttcatcgcg
     2041 ttcatgagcg cgcaagtctg ggagctgtgc cggccgaccc tgctcacggc gtttccaacc
     2101 ttgcaggtcg aaaacatgct tccgaacgga acccggctgg cagtcgaagt gccctagccc
     2161 ggcgattccg ccgtaactcg ggccatacga tctacccctc gctccagtcg attcccccgt
     2221 gattgggcgt ctcggtcaga aacgccggct cgcccacggt cgtgacaaac aacccgccta
     2281 agtgcatcaa tgccagccga tcgccatctg gcgaccagtc caacaccgtg ccgtccgtcc
     2341 cccattgtgt gatctgagtc ggctggccgc cggcgcttgg gactgtccaa aagtccgagg
     2401 gaagcagccc atgcgcgtag gcaacctcca ccccaaagag ccagtcccat agcgagcggc
     2461 tggtcggcgg cgggccaacc ggagggccgg cggccgcgaa cgcgatcgtc gctccgtccg
     2521 gagagagcct cggactcaag agatccggga aggtggtatc tgaaacgact tcgcgggcat
     2581 ccgtgccgtc gccgttggcg atccagagcc cgcgagtata cgtctctaca ttgaatcgga
     2641 agaacgcgag ccggctgccg tccgccgaga acgaaggttg ctccgcgttc tcgatgacct
     2701 ggtccggcgc gcctccgccc gccggaaccc g
//
LOCUS       DATDPQ010000202         5313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2432974, whole genome shotgun sequence.
ACCESSION   DATDPQ010000202 DATDPQ010000000
VERSION     DATDPQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432974"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VI520_04695"
     CDS             complement(<1..833)
                     /locus_tag="VI520_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HLF37216.1"
                     /translation="MLLTLIGGALACTIGIAPRSSLQATGEAASPVSGSPTPRASQAP
                     EEQSRPSTASPPAPTPSLVGGSGAIVYHRGPPGQEDIYLLPLDGSEPQALASESGSDI
                     SPRWSPDGHQIAFVSDRDGDREIFIMGADGSAPRNLTLNSAADDSPYWTPDGQSIVFS
                     SDRDGNREIYRMRIDGTNVKRLTFDLGFDVSPAVAPDGSQIAFVSNREGDSEVFLMDA
                     EGESPVRLTSGYGVARYPAWNPNGSTLVFASDRSGDFELYQIDLRGGEPENLTTNAAH
                     DA"
     gene            complement(859..1935)
                     /locus_tag="VI520_04700"
     CDS             complement(859..1935)
                     /locus_tag="VI520_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF37217.1"
                     /translation="MSDLVIYFGLLELAVVLIGSGAILAIILAVIWRGRMARRYQRLV
                     DGFPYPAMVFRRDRMILRNLESAGWDGRLELHDLVSDSRRERTNIVRTIPAVEGQSFQ
                     ARALRLTAVDTLLLLEDLGATQRQQSFYRNFIQNVSHELKTPLTVIQGHVAKMAAQPE
                     DREGWLASSRIVNEETKRLTQLVDNLLTLARLESQAFTLNLEPVNVAALLEESILQMS
                     ELAEGRKLEISLSLPPTSPRVTADRARLKQVVLNLLDNSLKYTSPGGQISVGLEDDRA
                     HRRLVCAVRDSGEGIPEADLPFIFDKLYRARRTGGRPVEGSGLGLSLAQQIIAVHGGE
                     IRVESELGKGSTFTFTLPYEAPTG"
     gene            complement(1994..2677)
                     /locus_tag="VI520_04705"
     CDS             complement(1994..2677)
                     /locus_tag="VI520_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37218.1"
                     /translation="MTHKILLADDNEKIISFVEPALQREGYEVITASDGEEALYLWET
                     GHPDLLVLDIEMPEPNGLSVCRRIRSVGDSVPIIFLTIRDSVSDLELGFSLGASDYVS
                     KPFEMGELLARIKARLPPAIQEYDGYLRVDTPRRKVMVQRQGQWTTVDLTPREFDLLN
                     YLVTNAGRPISRSSLLEAVFEIPDEQEIQTKTLEKHIWSLRHKIEPDPKEPRYLVNVR
                     GVGYKFEPT"
     gene            complement(2767..3513)
                     /locus_tag="VI520_04710"
     CDS             complement(2767..3513)
                     /locus_tag="VI520_04710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLF37219.1"
                     /translation="MPSPSPASQVAASATSGEWRIAGPGILDVPILLYHHIDPGRPPG
                     KFNVTPAQFELQVQSLAAGGFQSITPANLRQAVLHGAPLPARPIIITIDDGHENAYVY
                     AYPILEKYGFDAVIYVVANRLWSTGCLSAEQLIELSEAGWEVGSHSMTHADLTTRVGR
                     ELRREIFGSREQLEASLGQEVPSFAYPFGAFSPEVAGQVRAAGYQTAMGLGTSTLQGR
                     GNLYYLSRIPVNGSWTVSEFAAAVGFDIRP"
     gene            complement(3985..4737)
                     /locus_tag="VI520_04715"
     CDS             complement(3985..4737)
                     /locus_tag="VI520_04715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLF37220.1"
                     /translation="MSDALKPREETPPIAMELGVEGVVPEAELSPSLNVDCRTPQSLM
                     LHSYYGVERMRALADSILAGNMRTLTYQGILEGLRRGQCPPGNGIVVSLDDLSTAWID
                     PTLKEMIAAFTDRGLVLVVAVIVREPQDPETWAYLRELSELGVEIASHSLDHPNLPGL
                     SQTEMEWQVSGSYRAICENLGACPVSFVVPYGSMDRHQRIMGAAQEYSFVVGIAGGLR
                     IEGGAPYYVGRSGPDIKSIKRTLQVLEVAFGS"
BASE COUNT          967 a         1636 c         1776 g          934 t
ORIGIN      
        1 gcgtcgtggg cggcgttggt cgtcaggttc tccggctcac cgccgcgcag atcgatctgg
       61 taaagctcga aatccccgct acggtcggag gcaaagacaa gcgtgctgcc attcggattc
      121 caggctggat agcgcgccac cccgtagccg ctggtcagcc ggaccgggct ctcgccttcg
      181 gcgtccatca ggaagacctc gctgtcaccc tctcggttcg acacgaacgc gatctgtgag
      241 ccgtccggcg cgacggccgg acttacgtcg aatccgaggt cgaaggtgag gcgtttgaca
      301 ttcgtcccat cgattcgcat tcgataaatc tcgcggttgc cgtcccgatc cgaggagaac
      361 acgatggatt gcccgtccgg cgtccaatac ggggagtcat ccgcggcgga gttgagcgtc
      421 agattgcggg gcgcggagcc gtcggctccc atgatgaaga tctcccgatc gccatcccgg
      481 tcggagacaa acgcgatctg atgaccatcc ggagaccagc ggggcgagat gtccgagccg
      541 gattccgaag caagcgcctg cggctcactt ccgtccagcg ggagcaggta gatatcctcc
      601 tgacccggcg ggccgcggtg gtagacgatc gcgccggaac ccccgaccag agacggcgtc
      661 ggagctggcg gggaggccgt ggacgggcgg ctttgctcct cgggagcttg ggacgctcgc
      721 ggggtgggcg accccgaaac ggggctcgcc gcctcgccgg tcgcttgcag cgagctgcgc
      781 ggggcgatgc caatggtgca ggccagcgcg ccgccgatga gcgtcagcaa caagagggtc
      841 agccgcccgc agtacgggct agccggtggg tgcttcatag ggaagggtga aagtgaaggt
      901 cgagcctttg cccagctccg actcgacgcg gatctcgcct ccatgaacgg ctatgatctg
      961 ctgggcgaga ctgaggccca gaccagaccc ctcgacagga cgcccgccgg ttcggcgggc
     1021 gcgatagagt ttgtcaaaga tgaacggaag gtccgcctcc ggaattccct ccccgctatc
     1081 ccgcacagcg caaacgagcc tccgatgggc ccggtcgtcc tccagaccga ccgaaatctg
     1141 gccgccgggg gaagtgtact tgagcgagtt gtcgagcagg ttgagaacca cctgcttcaa
     1201 ccgggcgcgg tcagccgtca ctcgcgggct tgtcggaggc agactcaggc tgatctcaag
     1261 ctttcgcccc tcggccagct cggacatctg gagaatcgac tcctccaaca gcgccgcgac
     1321 attcaccggc tcgagattga gggtgaaggc ctgggactcg agcctggcca gcgtcagcag
     1381 gttgtcgacc aactgggtca ggcgtttcgt ttcttcgttc acgatccggc tcgaggcgag
     1441 ccagccctcg cgatcctccg gctgtgcggc catcttcgcc acgtgtccct gaatcacggt
     1501 caggggagtc ttgagttcgt gactgacgtt ttgaatgaag ttgcggtaga aggactgctg
     1561 acgctgcgtg gcgcccagat cctcgagcag cagcagcgtg tccactgcgg taagcctgag
     1621 cgcgcgggcc tggaaggact gaccctccac ggcggggatc gtgcggacga tgttggttct
     1681 ctcccgacgc gagtcagaga ccagatcatg tagctcgaga cggccgtccc acccggcgga
     1741 ctccaagttg cgcaggatca tccggtcgcg gcggaagacc atcgccgggt agggaaaacc
     1801 atccaccaga cgctggtagc gacgtgccat ccggccccgc cagatcacgg ccaggatgat
     1861 ggccagaatg gcaccacttc cgatgagcac cacagcgagc tcgagcagcc cgaagtagat
     1921 aactaggtcc gacatcgcca gacgagcttc agggccgcgc cggctgggcg ccgggcgatt
     1981 gaggccggta gcgtcacgtg ggctcgaatt tgtagcctac cccacgtacg ttgaccaggt
     2041 agcgaggctc tttggggtcg ggctcgatct tgtggcggag cgaccagatg tgtttctcca
     2101 aggtcttggt ctggatctcc tgctcgtccg ggatttcgaa gacagcctcg agcagggagc
     2161 tacgactgat ggggcggccg gcattggtca ccaggtaatt cagcaggtcg aactcacgag
     2221 gagtcaggtc gacggtggtc cattgaccct ggcgctggac catgactttc cgccgcgggg
     2281 tgtccaccct caggtaccca tcgtactcct ggatcgccgg aggcaggcgg gccttgatcc
     2341 gggccagcag ctcgcccatc tcgaagggct tggaaacata gtcgctggcc cccaggctga
     2401 accccagctc gaggtcgctg acgctgtcgc ggatcgtcag gaagatgatc gggacgctat
     2461 cgccgacgct tcgaatccgg cggcagacgg agaggccatt cggttcgggc atctcaatgt
     2521 cgagcacgag cagatcggga tgcccggtct cccacaagta gagcgcctct tcgccatccg
     2581 aggcggtgat cacctcatag ccctcccgct gcaaggcggg ttcgacgaag gagatgatct
     2641 tctcattgtc gtcggcgagc aggatcttgt gcgtcactgg cgcgattata gccacccgac
     2701 tgagtgggac aagccgtccg gctgatggcg tcagcggccg tatccgccgg cctcggctaa
     2761 gtagggtcaa ggtcgaatat cgaaccccac cgccgccgcg aattcggaca ccgtccacga
     2821 accattgact gggatccggc tcaggtagta gagattcccg cggccctgga gtgtgctcgt
     2881 tcccagaccc atggccgtct ggtagccggc tgccctcacc tggccggcca cctctgggct
     2941 aaacgccccg aaggggtagg cgaagctggg tacctcctgc ccgaggctgg cttcgagctg
     3001 ctcccgcgag ccgaagatct cgcgccgcag ctctcgtccg acacgcgtgg tcagatccgc
     3061 atgggtcatg ctgtgcgatc cgacctccca gcccgcttcc gagagttcga tgagctgttc
     3121 ggcggacagg caaccggtcg accaaagtcg gttcgccacc acgtagatca cggcatcaaa
     3181 cccatacttc tcaaggatcg ggtaggcata cacgtaggcg ttctcgtgac cgtcgtcgat
     3241 cgtgatgatg atgggtcggg cgggcagcgg cgcgccgtgc aggacggcct ggcgcaggtt
     3301 cgctggggtg atcgattgga agccgcccgc cgccagactc tggacctgca actcgaactg
     3361 cgccggcgtc acattgaact tcccgggcgg ccgcccaggg tcaatgtgat ggtaaagcag
     3421 gatcgggaca tcgaggattc ccggtccggc gatcctccac tccccgctgg tagcggaggc
     3481 ggccacctgg gaggcgggcg acggcgatgg caagggggat cccggaggtg ctaccgtcgg
     3541 tgatggcccg aagggtcgtg agggctttgc cgtaggcggg ctaacggcaa gatcgcttcg
     3601 cccgcgcagt gcctgcgagt tctgtaggct acgtgggccg gcagtgggcc tacggtcagc
     3661 gacgcccccg atccgggtct cggtcggcga tgccggatgg caagatggaa gcgaagccat
     3721 cgccaagatg atccacacaa gaacttgatc tcgcatttcg gcctcctggt cgtcgcttcg
     3781 acgttaggcg gccgagttca ccggtagggc acacgacgtt cacaagttgg ttaccagatc
     3841 agtaacctgg ctgtgtaagt gaagcatggg gcccggagga tttctccccc gggaagacag
     3901 ggggagttgc agagcgcatc gagatggtct ggtcggcagg agaaaggcgc gagggtgaaa
     3961 ctgcagagcc ggtccgagtc taggttaact gccgaacgcc acttccaata cctgcagcgt
     4021 gcgcttgatg gacttgatgt cggggccgct ccggccgacg tagtacggcg cgccgccctc
     4081 gatccgtagc ccaccggcga tgccgactac gaagctgtac tcctgggcgg ctcccatgat
     4141 ccgctgatga cgatccatgc tgccgtaagg gacaacgaac gagacggggc aagcgcccag
     4201 gttctcgcag atggcccggt acgaccccga aacctgccat tccatttccg tttgagagag
     4261 ccccggcaga ttagggtgat ccagactgtg gctggcaatc tcaaccccca actcgctcag
     4321 ctcgcgaagg taagcccaag tctccgggtc ctgcggttcg cgcacgatca ccgccaccac
     4381 cagcaccaat ccgcggtcgg tgaaggcagc gatcatttcc ttcagggtcg ggtcgatcca
     4441 ggccgtggag agatcgtcga gcgaaacaac gatgccgttc ccgggggggc attgcccccg
     4501 ccggagccct tccaggattc cctggtaggt cagggtgcgc atgttgccgg ccaggatgct
     4561 atcggcgagc gcccgcatcc gctcgacacc gtagtacgaa tgcagcatga gggattgcgg
     4621 tgtccggcag tcgacattca ggctcgggga aagttccgct tcggggacca caccctccac
     4681 gccgagctcc atagcgatgg gtggtgtctc ctcgcgcggc ttcaacgcgt cgctcaaggg
     4741 atcagttcct gccccgtcat tggctgaggc aggcgaggcg ccaggcgcca ctttgaggcc
     4801 ggccagcgtg gtcgcttgat tgatgggact ggattgttgg tagtggtcgt cgggcgccgg
     4861 cggggtaccc gttgcgcatg cgatcaagag cggcaggcag agaagcgctt ggatgagtag
     4921 tgcacgacgg gacacgggtg gcctccaagg tgcgatcgcg tttgagcgca tcctcggagg
     4981 ccgcgttcac cgcccgtttc ctggctggtt acgggaggat cacggatccg gtaaccgggc
     5041 ggtaaccgtt cggttggctt gaaaggcggc tgaaaggtcc ggggcaggca aaggcgtagc
     5101 aggcgcgccg ttaagcgctt gaagtctgga gtcgacccgt cagcctttgc gatgcgaagc
     5161 ggaagtgcgc ggccgcaagc acacccggct acgagacgca atatcgccgg gcggtccttt
     5221 ggcagcgccg cggtgcgggg gtctctccgc ccagaacctc gggcggcgat tcggctaggc
     5281 tagagggccg gagaacccac aaaggactgt cgg
//
LOCUS       DATDPQ010000203         3295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_87740, whole genome shotgun sequence.
ACCESSION   DATDPQ010000203 DATDPQ010000000
VERSION     DATDPQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87740"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VI520_04720"
     CDS             complement(<1..425)
                     /locus_tag="VI520_04720"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLF37221.1"
                     /translation="MLRLEKLKAGLPVGKLGVELHFYPSLGSTNDRAAELARAGAPEG
                     TLVVADEQTAGRGRSGRRWHSPAGSGLALSLVLRPTTLGSREIAAIGLIGAMGSIEAL
                     AEYGMQPELKWPNDVLLNGAKVGGILAEASWTGSELDHV"
     gene            547..1023
                     /locus_tag="VI520_04725"
     CDS             547..1023
                     /locus_tag="VI520_04725"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HLF37222.1"
                     /translation="MDYIEKQGENPGCLFCDRLAQEDGFNNLILHRGGQAFVILNRYP
                     YTSGHMMVVPIAHRPSIEDLDDDSLRELMQFSSKAVSVLRKAYGALAFNLGINIGAPA
                     GAGVVDHVHMHVVPRWSGDTNFMVTTAETRVIPEALADTYLRLRSLWSAASPDPRP"
     gene            1054..2262
                     /locus_tag="VI520_04730"
     CDS             1054..2262
                     /locus_tag="VI520_04730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLF37223.1"
                     /translation="MLNDRTREPVILSAVRTPIGKFRGVLRDLPATQLGALVVREAVR
                     RAGLAEGSQIDEVLMGNVVAAGLGQAPARQAAIGAGLPPTVGATTLNKVCGSGLKAGM
                     FASQAIKAGDADLIVAGGMESMSRAPFLVRGRGGELNYGHQSLEDSLLLDGLWDPFQQ
                     WAMGMAAEFIADEYEVTREAMDRWALDSHRKAATAQDAGKFEAEIVSVKVAGDDGKTR
                     MESRDETLRRDSSLEKLAALKPVFRSDGRVTAGNSPGLNDGAAAVVYSSREKAAALGL
                     KPIARVVGYAQAAVAPKEMFIAPARVIPSLLAKVGWTPAQIDLIELNEAFAAQVLADG
                     YALSDAGWDWAKVNVHGGAIALGHPLGASGARILTTLIYALRDRGLSKGIASLCLGGG
                     EAVAMAIELE"
     gene            2266..3117
                     /locus_tag="VI520_04735"
     CDS             2266..3117
                     /locus_tag="VI520_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLF37224.1"
                     /translation="MPAKKILVVGAGTMGHGIAQTAAVSGYHVILSDVDPAALERGKA
                     SIEKSAVKLAGKGVLSAEQLQAARSITTSIDLDPAAEVDLVIEAATENPEIKFRIFAE
                     LDRRAPPEAVLASNTSSISITRIGAATARPDRVIGMHFFNPVPLMSLLEIIRGLNTSE
                     ATLALAIEVGRRMGKEPIQAKDSPGFIANRILCPMINEAIFALQENLGTAQDIDTVMK
                     LGMSHPIGPLALADLIGLDVLLHIMQVLHRDLGDDKYRPAPLLRQLVDAGLLGRKTGA
                     GFFQYPG"
     gene            complement(3114..>3295)
                     /locus_tag="VI520_04740"
     CDS             complement(3114..>3295)
                     /locus_tag="VI520_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37225.1"
                     /translation="LQAASKPANPTSASDIRRRLISAPRNNLFWLEAQVSERAAAGLL
                     PSGLPGRMDLPIRVH"
BASE COUNT          596 a         1054 c         1033 g          612 t
ORIGIN      
        1 atgacatggt ccagctccga gccagtccag ctggcttcgg ccaggatccc gccaactttg
       61 gctccgttga gcaggacatc gttgggccat ttcaattcag gttgcatgcc atattcagca
      121 agcgcctcga tactgcccat cgctccgatg agtccaatcg ccgcaatctc gcggctccct
      181 aaagtggttg gccgcaacac caggctgagc gcgagcccgg aacctgccgg gctgtgccat
      241 cggcggcccg atcggccccg gccggcggtc tgctcgtctg cgaccaccag ggtaccctcc
      301 ggggctccag cccgagccag ctcggcggcc cggtcgttgg tcgaaccaag gctggggtag
      361 aagtgcagct cgacgcccaa cttccccacc ggcaatcctg ctttgagctt ttcaagcctc
      421 aacacgcggc cgattctaat ccggctgcag gaggcgtgcc actccggtga tacaatcctc
      481 gggcccaacc gggccggggg aatttcgaca acccactcga tggaccgcat ctgggcgcca
      541 tggcgaatgg actatatcga aaagcagggt gagaatcccg gatgcttgtt ctgcgatcgc
      601 ctggcgcaag aggacgggtt caacaacctg atcctgcacc gaggaggcca ggccttcgtc
      661 attctgaatc gctatcccta taccagcgga cacatgatgg tcgttcctat tgcgcatcgc
      721 ccttcgatcg aggatctgga tgatgattcg ttgcgcgagt tgatgcagtt ctcctcgaag
      781 gcggtttctg tgctgcgaaa ggcctacggc gctctggcct tcaatctggg gatcaacatt
      841 ggcgcgccgg ccggtgccgg agtggtcgac catgtccaca tgcatgttgt gcctcgctgg
      901 tccggcgata cgaatttcat ggtcaccacc gccgaaactc gggtcatccc ggaagcgctg
      961 gcggatacct acctgcggct tcggagcctt tggagtgcag cctctccaga cccgaggccc
     1021 tagggtcgct cgatactccc ccaagtctat acgatgctga acgaccggac ccgcgagcca
     1081 gttatcctga gcgcggtccg cactccgatc ggaaaattcc ggggagtgct gcgggacctg
     1141 cccgcaaccc agctcggtgc gctcgtcgtc cgagaggctg tgcggcgggc cgggctggcc
     1201 gagggatccc agatcgacga agtgctgatg gggaatgtag tggcagccgg gcttggccag
     1261 gcacccgccc ggcaggcggc catcggggcc ggcttgcctc cgaccgtcgg agcgaccacc
     1321 ctgaacaagg tctgcggctc ggggctcaag gccgggatgt tcgcctccca ggccatcaaa
     1381 gccggggacg cggatctcat cgtggccggc gggatggaga gcatgagccg ggcgcccttc
     1441 ctggtgcgcg gtcgaggggg tgagctgaat tacggccacc agtcgctcga ggattccttg
     1501 ttattggacg ggttgtggga tcctttccaa caatgggcaa tgggcatggc cgccgaattc
     1561 atcgccgatg agtacgaagt gacccgcgaa gccatggacc gctgggcgtt ggacagtcat
     1621 cgtaaggcgg cgacggccca ggacgcggga aagttcgagg ccgaaatcgt ctcggttaaa
     1681 gttgcgggtg acgacggcaa aacccgaatg gagtcgcggg atgagaccct gcgccgcgac
     1741 tcgtcgctcg agaaacttgc cgcgctgaag ccggtcttcc gatccgacgg ccgggtgacc
     1801 gccggcaact cgcccgggct caatgacggt gccgcggccg tcgtatattc cagccgggag
     1861 aaggctgcag ccctcggtct gaagccgatc gcccgggtcg tcggctacgc ccaagccgcg
     1921 gtggccccga aagaaatgtt tatcgccccg gctcgagtca tcccgtccct gctcgccaag
     1981 gtggggtgga ccccggcgca gatcgacctg atcgagctga acgaagcctt cgcggcccag
     2041 gtcctggcgg atggctacgc gctctctgat gccggctggg attgggcgaa ggtcaatgtg
     2101 cacggcggcg ccatcgccct cgggcatccg ctgggcgcct ccggggcgcg gatcctgacc
     2161 accctgatct acgccctgcg agatcgcggg ctctcgaagg gaatcgcctc gctctgcttg
     2221 ggtggcggag aggcggtcgc catggccatc gaactggagt aagccgtgcc ggcaaagaaa
     2281 atcctcgtcg ttggagccgg taccatgggc cacgggatcg cccagaccgc cgcagtctcg
     2341 ggctaccacg tgattctgtc cgacgtcgat cccgcggctt tggagcgtgg caaggcttcg
     2401 attgagaaat ctgctgtcaa actggccggc aaaggtgttc tctcggccga gcagctgcag
     2461 gccgcccgga gcatcaccac ttccatcgac ctcgatcctg cggctgaggt tgacttggtc
     2521 attgaagccg caactgagaa tccggaaatc aaattccgca tctttgcgga gctcgaccgg
     2581 cgcgcacccc ccgaagcagt gctagcttcg aatacctcgt ccatctcgat cacccggatc
     2641 ggagccgcca cggctcgccc ggaccgggtc atcgggatgc acttcttcaa cccggtaccg
     2701 ctcatgagcc tgcttgaaat catccgcggg ttgaacacct cggaggcgac tctcgccttg
     2761 gcgatcgagg tcggccgccg aatggggaaa gaaccgattc aagccaaaga ctcgcccggc
     2821 ttcatcgcga atcggattct ctgccccatg atcaatgaag ccatcttcgc ccttcaggag
     2881 aatctcggta cggcgcaaga catcgacacc gtcatgaagc tcggaatgag ccatcccatc
     2941 gggcccctgg cgctggccga cctgatcggg ttagacgtat tgctgcatat catgcaagtc
     3001 ctgcatcggg atctgggcga tgacaagtac cggccggcgc cgctgctgcg acagctggtc
     3061 gacgcgggct tgctgggccg taagaccggc gcgggcttct tccagtaccc tggctagtgc
     3121 acccggattg gcaagtccat tcgccccggc aatcccgacg gtagtaagcc tgctgccgcc
     3181 cgttcactca cttgcgcctc cagccaaaac aggttattcc tcggcgctga aatgagccgt
     3241 ctacgaatat cgctggcgct cgtcggattg gcgggcttgc tggccgcctg caact
//
LOCUS       DATDPQ010000204         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_352086, whole genome shotgun sequence.
ACCESSION   DATDPQ010000204 DATDPQ010000000
VERSION     DATDPQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352086"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VI520_04745"
     CDS             complement(<1..1104)
                     /locus_tag="VI520_04745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLF37226.1"
                     /translation="MVVKMPKLGFDMAEGLLVRWVKGEGDSVQKGEVLAEIETDKATI
                     EVEAEAGGVVHKHLVPEHTSVPVGAPIAVIGEPGERIDAAELVGQASGPAAAARQGIP
                     VPGKVMATESAEDGDGRLPGGVRASPLARRLAAEGGINLRDLAGSGPHGRIVKRDVQA
                     VLGAPGAAAPQRSFETERVPLSKLRATIGRRMTASKQQVPHFYVSADLDAGPLMALRR
                     DLNSRLPEDSKFSVNDFLVKAAALALAEFPNLNSGLEGDQIVRHGEINVGVAVALEEG
                     LLTVVVRQADQTPLRQLSSELRELVGRARAGRVRPGDIEGSTFTVSNLGMYQVDHFIA
                     IINPPEAAILAVGSVREEPVVQAGAIVPGTRLQV"
     gene            complement(1110..2081)
                     /locus_tag="VI520_04750"
     CDS             complement(1110..2081)
                     /locus_tag="VI520_04750"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HLF37227.1"
                     /translation="MPEITIREAISQALWEEMERDSRVIILGEEVGLWGGTYAVTRGF
                     YDHFGPERVRDTPIAEGVIVGAANGAAMAGLRPVAELMTINFAFLAMDQIVNGAAKIH
                     YMFGGQFRVPVVIRAVGGGGRQLGATHSQTPDAVFAHFPGLKVVAPGTPRDAKGLLKA
                     AIRTDDPVMFIEHATLYQTKGEVPEGDYVEPIGVAKVQRQGSDVTLITYSKMLQLSLQ
                     AAEQLAAEGVQAEIIDLRSLRPLDMGPAVASVKKTNRAVVVEEGWRSFGVGAEVASRL
                     SEEAFDYLDAPVKRVAQEEVPLPYNRELEQQALPQVDDILKAVREVL"
BASE COUNT          377 a          751 c          655 g          397 t
ORIGIN      
        1 gacctgcagc cgagtccccg ggacgatcgc cccggcctgc acaaccggtt cctcccggac
       61 gcttcctacg gccaggatcg cggcctccgg cggattgatg atggctatga aatgatcgac
      121 ctggtacatc ccgagattgg aaacagtaaa cgtcgagccc tcgatgtccc ccggacggac
      181 tcgcccagcg cgggctcggc cgaccagttc gcgcagttct gaggagagtt gacgcagcgg
      241 ggtttggtca gcctgccgga cgactaccgt gagcagccct tcttccaagg cgacggcaac
      301 tccaacgttg atctcaccgt gccggacgat ctgatcgccc tcgagcccgg aattgaggtt
      361 cgggaactcg gccaaggcta gagcggcagc cttgaccagg aaatcgttga ccgagaactt
      421 agagtcttcc ggcagccgcg agttcaaatc tctgcgcagg gccatgagcg ggccggcatc
      481 caaatcggca gaaacataga agtgcgggac ctgctgtttg gaagccgtca tccgccggcc
      541 gatggtcgcc cgcagcttgg agagcggcac ccgttcggtt tcaaagctgc gctgcggagc
      601 cgcggccccc ggagcgccca acacggcctg cacgtcgcgt ttgacaatgc gaccgtgcgg
      661 accgctgcct gccagatcgc gtaagttgat tccaccctcg gcggccagcc gtctggccag
      721 cggcgaagcc cgcacgcctc ccggcaggcg gccgtcgcca tcttcggcac tctcggtcgc
      781 catcaccttt ccgggtacag ggatcccctg cctggccgcc gcagctggac cggaagcctg
      841 cccgacgagc tccgcggcat cgatccgttc gccaggctct ccgatcaccg cgatcggggc
      901 tcccaccggc acggaagtgt gctctggcac caggtgtttg tgcaccaccc cgccggcctc
      961 ggcctctact tcaatcgtag ccttgtcggt ctcgatctcc gcaagcactt cacccttctg
     1021 cacggaatcg ccctcgccct tgacccaacg gaccaacagt ccctcggcca tgtcaaagcc
     1081 gagcttgggc atcttcacga ccatagccat caaagcacct cgcgcacggc cttgaggatg
     1141 tcgtcgacct gggggagggc ctgctgctcg agctcccggt tgtagggtag gggcacctct
     1201 tcttgggcga cccgcttcac cggcgcgtcc aggtaatcga aggcttcttc gctcaaccgg
     1261 ctggctacct cggcgccgac tccgaaagag cgccaccctt cctccaccac gacggcccgg
     1321 ttggtctttt tgactgaggc aaccgccggt cccatgtcca gcggccgaag cgatcgcagg
     1381 tcaatgatct cggcctgcac tccctctgcc gcgagttgct cggcagcctg caaagacaac
     1441 tgcagcatct tagagtatgt gatgagggtg acatccgaac cctggcgctg cactttcgca
     1501 acaccgatcg gctcgacgta gtccccttcg ggaacctcgc ccttcgtctg gtagagcgtg
     1561 gcgtgctcga tgaacatcac cgggtcatcg gtccgaatgg cggccttaag caggcccttc
     1621 gcgtcgcggg gagtgcccgg ggcgaccacc ttgagccctg ggaagtgggc gaacacggca
     1681 tccggggtct gagagtgagt cgcaccgagt tgccggccgc cgccgcccac ggctcggatc
     1741 acgaccggga cccggaactg tccgccgaac atgtagtgga tcttggccgc cccattcacg
     1801 atttggtcca tcgccaggaa ggcgaaattg atcgtcatca gctccgccac cggccgcagc
     1861 ccggccatcg ctgccccgtt ggcggcgccg acgatcactc cttcggcgat cggagtatcc
     1921 cgcacgcgtt ccggaccgaa gtgatcgtag aaccctcggg tgacagcgta ggtaccgccc
     1981 cacaggccga cttcctcacc gaggatgatg acccgcgagt cgcgttccat ctcctcccag
     2041 agggcttgcg agattgcctc ccggatggtg atctcaggca ttcagtcttc cgtgtagatg
     2101 tcggtataga ggtcggcagg caccgggtcc gggctgctct cggcgaattc gatcgcggcc
     2161 aggatttcct tttcgacccg
//
LOCUS       DATDPQ010000205         6164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1144359, whole genome shotgun sequence.
ACCESSION   DATDPQ010000205 DATDPQ010000000
VERSION     DATDPQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144359"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1259
                     /locus_tag="VI520_04755"
     CDS             135..1259
                     /locus_tag="VI520_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLF37228.1"
                     /translation="MRRVAGLALSLDKKNLWILPAPMIGSLLLLLLSGCGAPSQSLLP
                     KPLPIAGQISEETAPSVPAVLPVVGSAAQPIATQSIVDLPEAAPNPVSDWRPPPYAIP
                     LAIRPTDHYYFGRPVPSGEVNWPNPRYRYGSTAFGEETIHTGVDLGAERDTPVLAAGA
                     GEITWVGYGLYRGVFDLKDPYGLAVAIRHDFGHEQQLLYTIYAHMSSTVVWPGQRVAA
                     GEQIGTVGDTGHASGPHLHFEVRLGENRYFASQNPELWTVPAEGWGVLAGRVSDTYGR
                     ELPEFRVQVKSVETEQIWEVWTYALGTVNSDEYYQENFVIGDLPAGPYQLEVLFLGRS
                     YSAFMYVYPGQTNFVRFQGRQGLTVEPQAHAALSLIPPYP"
     gene            1340..2176
                     /locus_tag="VI520_04760"
     CDS             1340..2176
                     /locus_tag="VI520_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF37229.1"
                     /translation="MLRERVSDNVYVFTSEIYAQVNAGAVVGPDWSVLIDTLAYPEEI
                     LELKGFLEDRLGRPVRYIINTHHHTDHTLGNSFFPRATILSHALCREYLEARGRQALE
                     IAKAANPELKDIRLKVPEVTFLNGRATIRVGRRALQVLPLPGHSADGVGVLVAEDRVL
                     FSGDVMMPIPTLADGDYDQMVASLKSIPRMKLENLVQGHGESVMRGEVNAAVRSNLAY
                     LGAIRRHVRKAARLKDPEGYLRSIDIEQCGKRRILMNGLAPELHTRNLIALLRKWYRR
                     PG"
     gene            2179..2589
                     /locus_tag="VI520_04765"
     CDS             2179..2589
                     /locus_tag="VI520_04765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HLF37230.1"
                     /translation="MAASRQVVLTFDGGSLGNPGQAYGSFRLSPGRGKNPPPTRLEFG
                     YGTNNQAEYQALIAGLEAVLLWAVKGGHDPRSIMVELRGDSQLVLNQLSGEWKVKHPD
                     LRRLHSRARSLIDQFGRVEFTHQPRAVTVRVLGH"
     gene            2950..>6164
                     /locus_tag="VI520_04770"
     CDS             2950..>6164
                     /locus_tag="VI520_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLF37231.1"
                     /translation="MFKPVASKVDFVTQEHEILKFWESTRAFQRQRQLRQNAPTWSFI
                     DGPITANNPMGVHHGWGRTYKDLFQRFKSMQGYQTRFQNGFDCQGLWVEVEVERERGF
                     KTKRDIEKFGLAEFVVLCKQRVLEFAALQTEQSIRLGYWMDWDSPAWLRELKYQLGTD
                     PAGWVTFSGPGGRIEGTVESVIGQLGLPELGGSYFTFSDENNYTIWSVLKSCYDRGWI
                     YKGRDVMPWCGRCGTGLSQHEIVTEGYQELTHPGLTLRLPLLERPGESLLVWTTTPWT
                     LTSNVAAAVGPELDYLRVRQGEEVLYLSKGTLGTLVGEYQVEAELKGAALEGWRYQGP
                     FDELPAQVESGSPLAHRVILWEEVSEAEGTGIVHIAPGAGAEDYQLGKRYGLPVIAPL
                     NEAGVFVEGFGWLTGMAVAETAEPIYQELVGRGLAYSRRDFRHRYPVCWRCGTQLVFR
                     LVDEWFISMGAQLDKPYGEVSEQEKQGNLRYQMMEAVIDQTRWYPSFGFDRELDWLRN
                     MQDWMISKKRYWGLALPIYECEECSNFEVLGSKAELKQRAIAGWEQFQGHTPHRPYID
                     AVQIACKECGKPVSRIPEVGNPWLDAGIVAMSTLGYNHDRDYWRKWFPADLISESFPG
                     QFRNWFYSLLAMSTVLERRAPFKNVFSYATLVAEDGRAMHKSWGNSIEFNEAADKMGV
                     DVMRWLYCDHPPEKDLLFGYQRADEVRRSFLIPLWNVYSFLVTYAAIDGWQPGPDRPA
                     SRVVLDRWIRARLSELIEAVSSALERYEPNSATARVNEFLDDLSNWYLRRSRRRFWAK
                     TGAGPGSDSDKADAYQTLYAVLVELVKVLAPFVPFVSEVIYQNLVRGVDPQAPESVHH
                     CSWPQPDPAHADPDLTDRMRLVKRLVSLGHAARNQAGVRLRQPLAQAAFTVRDPAEQE
                     TLGQFAELIAEELNVKSVRLLDMAGEAAEFQLKALPKQLGQKYGARFPAIRQAIQQLS
                     AEKFVQSLARGEPLTVTVEGKPIELLPSELELIVSPKAGFSTAAEGPYLAALTVEIDA
                     ELRNEGLAREFVRRVQELRKQAGFAVEDRIHIQYASTLELGT"
BASE COUNT         1188 a         1830 c         1998 g         1148 t
ORIGIN      
        1 cactccgtat cagaccccga ccccaactcc aaccaacact cctcggccga ctccaacccc
       61 atggtggtgg cgctggcgga ctccgactcc gactccaaat cccactgcca cctctaccgt
      121 gcagccgtag tcccatgcgg cgagtcgctg gtcttgccct gagcctcgac aagaaaaacc
      181 tgtggattct gccggcgccc atgatcggca gcctgctgtt gttgttactc tccggatgcg
      241 gcgcgccgag ccagagcctg ctgcccaagc cgctgccgat cgccgggcag atttcggaag
      301 agacggcccc gagcgtcccg gcagttttgc cggttgtcgg ctcggccgcg cagccgatcg
      361 caactcaatc catcgttgac ctgccggagg ccgctccgaa tccggtctcc gattggcgtc
      421 caccccccta tgccatcccg ctcgcgattc gcccgaccga ccactactac ttcggccggc
      481 cggttccctc gggggaggtc aactggccga atccgcgcta ccgctatggc agcacggcct
      541 tcggggagga gaccattcac accggtgtgg atcttggagc agagcgagac acgcccgtgc
      601 tggcggcggg ggccggcgag ataacctggg tcggatacgg cctctaccgg ggggtattcg
      661 atcttaagga tccgtatggc ttagccgtgg cgatccgtca tgattttggg catgagcagc
      721 aactgctgta cacgatttat gctcacatgt cgtccacggt cgtctggccg ggacagcggg
      781 tggcggccgg agagcagatc gggactgtcg gggataccgg tcacgccagc gggccccacc
      841 tgcatttcga ggtccgtttg ggcgaaaacc gttactttgc gagccagaat cctgagcttt
      901 ggacggttcc cgccgaaggg tggggagtgt tggcggggag agtgagcgac acgtacggcc
      961 gcgagctgcc cgagtttcgg gtgcaggtca agtcggtaga gaccgagcag atttgggaag
     1021 tgtggaccta tgcgctcggc acggtgaatt cggacgagta ttaccaggag aactttgtga
     1081 tcggcgacct gccggccggc ccctaccagc tcgaggtctt gttcttgggg cgatcctatt
     1141 ctgcgttcat gtacgtctat cccgggcaga ccaacttcgt ccggttccag ggccgacagg
     1201 gcctgaccgt cgagccgcag gcccacgccg cactctcgtt gatccccccg tacccctgac
     1261 cagccattgc ttatgtaaaa ccaacttgac tggccgctgc cggtcgtgat attcttcacg
     1321 ctcgctgggg cccgaccgat tgctgcgcga gcgcgtctcc gacaacgttt acgtcttcac
     1381 cagcgagatc tatgctcaag tcaatgcggg cgcggtcgtt ggcccggatt ggtcggtgct
     1441 gattgacacc ctggcatatc cagaggaaat cctcgagctc aagggcttcc tggaggatcg
     1501 gctgggcagg ccggttcgct acatcatcaa cacccatcac cacactgatc acaccttagg
     1561 caacagtttc ttcccccggg caacgatctt atcccatgcc ctgtgccggg agtatctcga
     1621 ggccagaggt cgtcaggctt tggagatcgc caaggctgcc aacccggagt tgaaggacat
     1681 tcgcctgaaa gttccggagg tcacgttctt gaatgggcgg gccacgattc gggtgggtcg
     1741 acgtgcgctt caggtcctgc cgttgccggg acacagtgcg gacggggtcg gggtgctggt
     1801 tgctgaagac cgggtgctct tctcgggtga tgtcatgatg cccattccta ccctggccga
     1861 tggcgactac gatcagatgg tggccagcct gaagtccatc ccccggatga aattggagaa
     1921 tctggtccag ggacacggcg aatcggtgat gcgaggcgag gtcaacgcgg cggtgcgctc
     1981 caatctcgcc tacctgggag cgatccggcg gcacgtccgc aaggcggcgc ggctgaagga
     2041 ccccgaggga tacctccgct cgatcgacat tgagcagtgc ggcaagcggc gaatcctgat
     2101 gaacggcctc gcccccgagc tacacactcg caatctgatc gccctccttc ggaagtggta
     2161 ccgaaggccc ggctgaacat ggctgcgagc cgccaggtgg tgctgacctt tgatggaggc
     2221 agcctaggga acccaggcca ggcctatggg tcgttccggc tctcaccggg ccgggggaaa
     2281 aaccctcctc caacccgcct cgaattcggc tatggcacga acaaccaagc cgagtatcag
     2341 gcgctgatcg ccgggttgga ggcggtgctg ttgtgggcgg tgaaaggtgg acacgatccg
     2401 cggtcgatca tggtcgagct gcgcggggac agccagctgg tgctcaatca gctctccgga
     2461 gagtggaaag tcaaacaccc agatctgcgg aggctccatt cccgcgcccg ctcgctgatc
     2521 gaccaatttg ggcgagtcga attcactcat caaccccggg cagttaccgt gcgagtgcta
     2581 ggacattgac cggccgccag ccacatgaga taattcgctc ggtccaaagg cagcgaaggg
     2641 gaagagtagg ttcgtcagtc accgttagcg agcccgggag agtggaagcc gggccggagc
     2701 ggcgagccga atatcacctc ggagccgcga cctgaaccgg tttgtagggg agccggtctc
     2761 gcccaccgtt agccgggcgc gggtagtcgc ctgagccggc cgtacccgac aaagtgcccg
     2821 ccaacggcgg gaagcagggt ggtaccgcgg gagcgctgta gctctcgtcc ctgacaagga
     2881 cgggagcttt tcatttacaa gagcaaaggc cggatcgatc aacgatggat cagcgaactg
     2941 gaggagtgag tgttcaaacc ggttgccagt aaggtcgact tcgtgacgca ggagcacgag
     3001 attctgaagt tctgggagag cacccgtgcc ttccagcgcc agcgtcaact gcgccaaaac
     3061 gccccgactt ggtccttcat cgatggcccg atcaccgcca acaacccgat gggggtgcac
     3121 cacggctggg gccggactta caaagaccta tttcaacggt tcaagtcgat gcagggttac
     3181 cagactcgct ttcaaaacgg attcgactgc caggggctgt gggtggaagt agaggtcgag
     3241 cgcgaacgag gcttcaagac gaagcgggac atcgagaagt ttgggctcgc ggaattcgtc
     3301 gtactctgca agcagcgggt gcttgaattt gccgcgctcc agaccgaaca gagcattcgc
     3361 ctcgggtatt ggatggactg ggacagccca gcttggctgc gcgaactcaa gtaccaattg
     3421 ggaacggacc ccgctgggtg ggtcacgttc agcggaccgg gcggccggat cgaagggacg
     3481 gtcgagtcgg tgatcggaca gctcggactg ccggagctcg gcgggtccta ctttaccttt
     3541 tccgatgaaa acaactacac gatctggtcg gtcttgaaat cttgctacga tcgaggctgg
     3601 atctacaagg gcagagatgt gatgccgtgg tgcggccgct gcggcaccgg cctctctcaa
     3661 catgaaatcg tcaccgaggg atatcaggag ctcacccatc ccgggctgac cctgcgtttg
     3721 ccgctgctgg agcgtccggg cgagagcctg ctggtttgga ccaccacgcc ctggaccctg
     3781 accagcaacg tggccgctgc ggtcgggcca gagctcgact acctgcgggt gcggcagggc
     3841 gaggaagtgc tgtatctgag caaaggcacc ctggggacgt tggtcggtga gtatcaggtg
     3901 gaggctgaac tgaaaggggc cgcgctggaa ggttggcgct accaggggcc cttcgatgag
     3961 ctgccggccc aggtcgagag cggatcgccg ctcgcccacc gagtgatcct ctgggaggag
     4021 gtgagtgagg cggagggcac cggcattgtc cacattgccc ccggcgccgg cgcggaagac
     4081 tatcagttgg gaaagcgata cggactgccg gtgatcgcgc cgctcaacga agcgggcgtc
     4141 ttcgtcgagg gcttcggttg gctgaccgga atggcggtcg ccgagacggc cgagccgatc
     4201 taccaagagc tggtcgggcg aggcctggcc tatagtcggc gcgactttcg ccaccgctat
     4261 ccggtgtgct ggcgctgtgg tacccaattg gtttttcgat tggtcgacga gtggttcatc
     4321 tccatgggtg cgcagctcga taagccgtac ggggaggtca gcgagcagga aaagcaaggc
     4381 aacctgcgct atcagatgat ggaggcagtc atcgatcaga cccgctggta cccgagtttt
     4441 gggttcgatc gggagctgga ctggctgcgc aacatgcagg actggatgat ttcgaagaaa
     4501 cgctactggg ggctggcgct gccgatctac gagtgcgagg agtgcagcaa ctttgaagtg
     4561 ttggggagca aagcggagct gaagcagcgc gcgatcgccg gttgggagca gttccagggg
     4621 cacaccccgc atcggccata catcgacgcg gtgcagatcg cctgcaaaga gtgcgggaag
     4681 ccagtcagcc gaattccgga agttgggaat ccgtggctgg atgccggcat cgttgcgatg
     4741 agcacgctcg gctacaacca cgaccgggat tattggagga agtggttccc ggccgatctg
     4801 atcagcgaga gctttcctgg ccaatttcgg aattggttct acagcttgct ggccatgagc
     4861 acggtgctcg agcggcgtgc gcccttcaag aacgtcttct cctacgccac cctggtcgcc
     4921 gaagacggcc gggccatgca caagtcatgg ggcaactcga tcgagttcaa cgaggcggcg
     4981 gacaagatgg gggtggacgt catgcgctgg ctgtactgcg accacccacc ggagaaggac
     5041 ctgctcttcg ggtaccagcg ggccgatgaa gttcggcgca gttttctgat tccattgtgg
     5101 aacgtctaca gcttcttggt gacctacgcc gccattgacg gatggcagcc cggcccggat
     5161 cgcccagcat caagggtggt tctggaccgc tggatccgag cccggctcag tgaactgatc
     5221 gaggcagtca gctctgcgct cgagcgatac gaacccaact cagccaccgc ccgggtgaat
     5281 gaattcctgg atgacctgag caattggtac ctgcgccgca gccggcgacg cttctgggca
     5341 aagacgggag ccggaccggg gagcgattcc gacaaggccg acgcatacca gaccctttac
     5401 gcagtgctgg tcgagctggt gaaagtgttg gcgccattcg ttccatttgt gtccgaagtg
     5461 atctatcaaa acctggtccg gggagtcgat ccccaggccc ccgagagtgt ccaccactgc
     5521 agttggccgc agcccgatcc ggcgcacgcc gatcctgatc tgaccgaccg aatgcggctc
     5581 gtcaagcggc tggtctcgtt ggggcatgca gcccggaacc aggccggagt gcggcttcgg
     5641 cagccattgg cgcaggcggc ctttacggtc cgcgatcccg ccgagcaaga gacgctgggt
     5701 cagttcgccg agctgatcgc cgaagaattg aatgtgaagt cggttcgact gctcgatatg
     5761 gccggtgaag ccgctgaatt ccagctcaag gccctcccga aacagctcgg tcaaaaatac
     5821 ggggcccgct ttcccgcgat ccggcaggcg atacaacaac tctccgccga gaagttcgtt
     5881 caatcgttgg ctcggggtga accattgacg gtcaccgtcg agggcaaacc gattgagttg
     5941 ctgccctcgg aactcgagct gatcgtgtcg ccgaaggcgg gattctcgac cgcggccgag
     6001 ggcccttacc ttgccgcctt gacagtcgaa atcgatgccg aactgcgtaa cgaggggctg
     6061 gcccgggagt ttgttcgcag ggtccaggaa cttcgcaaac aggccggctt cgcagtcgaa
     6121 gaccggattc acatccagta cgcttcgacc ctcgagctgg gcac
//
LOCUS       DATDPQ010000206         3457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3485423, whole genome shotgun sequence.
ACCESSION   DATDPQ010000206 DATDPQ010000000
VERSION     DATDPQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485423"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VI520_04775"
     CDS             <1..986
                     /locus_tag="VI520_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37232.1"
                     /translation="FAAAATALATGWVLLLPQPHPRWDSWLMAVSTLLVIGLSVSVGI
                     EQLAGTSWPVRLWSGFGLLGTGGLRSAEAVSYPVVALLIVQKLIRAGVEQPGPGTKPE
                     LEASSTSKPEPVKAWAEKFGHRIGGIAESAPGSEPPGQMETLKAELRLALEELAELKS
                     QTERQRPIPLASLTSPIEGRADVISSVETAVAQVAGSLKERDQSLHLDLAGSLPSVSG
                     DPDELRQILVTLIENAIQASPAGAVIPVSALATDSADGAWVSLSVSDSGGGIPPEDLG
                     RVFHSPEPGRGTESGLARVKQLAEGMGGRVWVGSEVGGGSTVTVLIPARSE"
     gene            994..2403
                     /locus_tag="VI520_04780"
     CDS             994..2403
                     /locus_tag="VI520_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HLF37233.1"
                     /translation="MTRGGLVSLLLLLAAACVPAPKVDPGAPRLQKPTAQSSAAAPLS
                     TPTPESRASPQPLPDLGWPAPFTYGNPPSPPTPIPPPSPQLGFDREVVNILLLGSDRR
                     PSSSSFRTDVLIIVSIHPEKGAVAMLSIPRDLYVYLPGYSMQRINTAFSLGETIGYPG
                     GGPGLLADTIRYNLGIQIDHFARVEMDGFSRIVDTLGGVEVSVTCSYRDWRLKEPFLN
                     PNLAQNWRLHTVPAGVIQMDGDLALWYARSRSLSSDFDRSRRQQEVLRAIYRQVLSFD
                     LIPRLPALYSELSDTLTTDLGLADLLTLAPLSARISSADIRSRFIGRDEVTSWRVPVS
                     GAQVLVPRPESLQALVAAAFDFEAPDELVPEIALTVEVINSSNDPTWGLLAAERLNYA
                     GFEAFASPAASAPASTTQVIDFGLATAEASQAILRALGFGSERLTSSPDPTSPFVYRL
                     VIGNDYDPCFDPTRGQLGS"
     gene            2483..3433
                     /locus_tag="VI520_04785"
     CDS             2483..3433
                     /locus_tag="VI520_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLF37234.1"
                     /translation="MAATREQLPLFSRSRRENRSNQGRPRGPVRPDSSLSAAIQAWGE
                     DLEAAGRSIHTVKAFSADLRLLARFMGAGHSINAIGQHDLRNFLEWMLNRRGVPCSPK
                     TYSRRVTSLKAFFRWLKQAGVLIEDPAAPVPQQSVLSPLPEVLTQDEVLAVLKTAEQL
                     RRKDPPDARPYALVGLLLTTGVKKGECLGVHLKHIDLKAVDGPILWIRYGDVRKRYRE
                     RKLPLEPAWIPAYREYLGQYSPTERLFPYSPRRLEYLLEEIGASAGLRKKHLSFNMCR
                     WTAALTDYKAGIDKDNLRQKLGVSKIQWREVGLKLDRLAA"
BASE COUNT          616 a         1095 c         1081 g          665 t
ORIGIN      
        1 ggttcgccgc ggcggcaacg gccttggcca ccggttgggt gttgctcctt ccgcagccgc
       61 acccgcgctg ggactcctgg ttgatggcgg tcagcacgct gctcgtgatc gggctcagcg
      121 tctcggtcgg cattgagcag ctggcgggga cctcttggcc ggtaagacta tggagcggat
      181 ttggtctgct cggcaccggc ggattgcggt ccgccgaagc ggtctcttac ccggtagtcg
      241 cgctgctcat tgtccaaaag ctgatccggg caggtgtcga acaacccgga cctgggacga
      301 aacccgagct cgaagcaagc agcacgagca agcctgagcc ggtcaaggcc tgggccgaga
      361 agttcggtca ccggatcgga ggcattgccg agagtgcacc cggctccgag ccccccgggc
      421 aaatggaaac cctcaaggcg gagctgcggc tcgccctgga ggagctggcg gagctcaaga
      481 gccagaccga gcgtcagaga cctattccgt tggcttcatt gacctcaccg atcgaggggc
      541 gggctgacgt gatcagctcg gtagaaacag cagtcgctca ggtcgccggt agtctcaaag
      601 agcgagatca atcgcttcac ctggacttag ctgggagcct tccgtcggtg agcggcgacc
      661 cggacgaatt gcgtcagatc ctggtgaccc tgatcgaaaa cgcgattcag gcctccccgg
      721 ccggagcggt gattccggta tctgcactcg ccaccgattc ggctgatggt gcatgggtga
      781 gtctctccgt cagcgactcc gggggcggga ttcctccgga ggacctgggg cgggttttcc
      841 atagccctga gccggggcgc ggaaccgaga gcggcctggc tcgggttaag cagctggcgg
      901 agggaatggg gggaagagtc tgggtcggca gcgaagtcgg agggggcagc acggtcaccg
      961 tattgatccc ggcccgatcg gaatgagcca acgatgactc gaggcggtct tgtttcgctg
     1021 ctgctgttgc tggctgcggc ctgcgttccg gctcccaaag tcgaccccgg cgccccgcgg
     1081 ctgcagaaac cgacggctca gtcttccgcg gctgcgccgc tgagcactcc gacgccggaa
     1141 agtcgggcga gtcctcagcc attgccggac ctcgggtggc cggcgccctt cacctacggc
     1201 aatccaccct cgcctcccac cccgatccct ccgccgtccc cgcagttggg tttcgatcgg
     1261 gaagtcgtga acatcctgct gctcggatcc gaccggcggc cctcaagcag cagtttccgc
     1321 accgacgtct taatcatcgt atcgatccac cccgagaagg gggcggtggc gatgctctcg
     1381 attccgaggg acctctacgt ttacttgcct ggttacagca tgcagcggat caacaccgca
     1441 ttctcgctgg gtgagaccat cggatatccg ggaggcgggc cgggcctgtt ggcggatacc
     1501 atccgctaca accttggaat ccagatcgat cacttcgccc gagtcgaaat ggacgggttc
     1561 agccggatcg tggacacact gggcggggtc gaagttagcg tgacctgttc ttatcgggac
     1621 tggcgtttga aagagccctt tctgaacccc aacctcgccc agaactggcg actacatacc
     1681 gttccggccg gagtcatcca gatggacggc gatctggcac tttggtacgc ccgctcgcgc
     1741 agtttgtcct ccgacttcga tcgcagtcgg cgacagcagg aggtcctgcg cgcaatttac
     1801 cggcaggtct tgagcttcga tctgattccg cgactccctg ctctgtactc ggagctgagc
     1861 gacaccctga ccaccgacct ggggctggcc gacttgctca cactcgcgcc gttgtcggct
     1921 cggattagca gcgcggacat ccggagtcgt ttcatcgggc gggatgaagt caccagctgg
     1981 cgagtcccag tcagcggagc gcaagtgctc gtgcccaggc ccgaatcgct gcaggcttta
     2041 gtagcggcag ccttcgactt cgaggctccc gacgagctgg tccctgagat tgcccttacg
     2101 gtggaggtga tcaactcttc caacgacccg acctggggcc tgctggccgc cgagcggctg
     2161 aactatgccg gatttgaggc attcgcgtct ccggcggctt ccgcacccgc aagcacgacc
     2221 caagtcatcg atttcggctt ggcgaccgcc gaggcaagcc aagcgatcct gcgggcgtta
     2281 ggctttggga gtgaacgcct gacaagctcc cctgacccta cgtcaccctt tgtttatcgt
     2341 ttggtgatcg gaaacgatta cgacccgtgc ttcgacccca cccgcggcca actcgggtcg
     2401 tagtcagcca gccctatttg acttttcgca acctcgagtc tagtatccaa tccttggcta
     2461 tgcgaagcag tctcggatag cgatggccgc aactcgggaa cagcttccgc tcttctcgcg
     2521 atcccggcga gaaaaccgtt ccaaccaggg gcgcccccgc ggacctgtgc ggccggattc
     2581 cagcctgagc gccgcgatcc aggcctgggg ggaagacttg gaggcggcgg ggcgctcgat
     2641 ccacaccgtc aaggcctttt ccgctgacct gcgcctgctc gcccgtttta tgggcgccgg
     2701 ccactccatc aacgcgatcg gtcaacatga cctgcgcaac ttcctggaat ggatgctcaa
     2761 tcgtcgggga gtgccatgca gtcccaagac ctactctcgg agggtgacct cgctcaaggc
     2821 gttcttccgc tggctcaagc aggccggcgt gctgatcgag gatccggcgg ctcccgttcc
     2881 acagcagtcg gttctcagcc cgttgcccga ggttcttacc caggatgagg tcctagccgt
     2941 actgaagacg gccgagcagc tgcgacggaa ggatcctccg gatgcgaggc cctacgccct
     3001 ggtcggtttg ctattgacta cgggggtgaa gaaaggcgag tgcctggggg ttcacctcaa
     3061 acacattgac ctcaaggcgg tggacgggcc catcctgtgg atccgctacg gggacgtccg
     3121 caaacgctac cgggagcgca aactgccgct cgagccggct tggattccag cctaccgaga
     3181 gtatttggga cagtattccc cgaccgagcg cctgttcccc tactctccgc gtcggttgga
     3241 gtatctgctc gaggagatcg gagcctcggc cggattgagg aagaaacatc tttccttcaa
     3301 catgtgccgc tggacggccg ccctcaccga ttacaaggcc gggatcgaca aagacaatct
     3361 gcgacagaaa ctcggtgttt ccaagattca gtggcgcgag gtcggtctca agctcgaccg
     3421 gctggccgcc tgaaccctac atcttcttta cgacgat
//
LOCUS       DATDPQ010000207         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3244658, whole genome shotgun sequence.
ACCESSION   DATDPQ010000207 DATDPQ010000000
VERSION     DATDPQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244658"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VI520_04790"
     CDS             complement(<1..558)
                     /locus_tag="VI520_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLF37235.1"
                     /translation="MDLLHGLNSQQREAVTAPLGPALVLAGPGSGKTRVLTHRLAYLI
                     QELGIRPEQIVALTFTNKAAREMEKRAGSLLGQQQTQAAQPRLGTFHAFFARMLRIEA
                     DRLPVSRDFVIFDEADQLALVRQSLKDLNLDPQRIQPGQVLGAISRAKNELIAPAALT
                     TDTYFTEITRRVYERYQELLLVNNAL"
     gene            complement(626..1789)
                     /locus_tag="VI520_04795"
     CDS             complement(626..1789)
                     /locus_tag="VI520_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HLF37236.1"
                     /translation="MADCLRAIRDALGGAGYETQTLRLATPPPLQIDKPVSPRERPEL
                     ARQLAAECATHGIEYANIGPALAEELEGFRVVPEVLAAAESVFCSGLYASLPSGLSLP
                     AARACAEAIRAASTVSPNGFANLRFAGLANVGPGSPFFPAAYHDGGPPAVALATESAD
                     LVVESFRNATSLATVRWQLVSSIETHAAALTRAAEPVAKDRSVRFLGVDFSPAPYPEP
                     DRSLGTALELLGAPALGLSGTAAAAAFLADCLDQAKFARTGFCGLMLPVLEDSILAMR
                     AAEGRLGLSELLLMSTLCGCGLDTIPLPGDISKESLAALLIDLGALALRHNKPLTARL
                     MPIPLKSAGDPIHFDFPYFADSRVMEFPAMPLTNLLASSDLLELSPRPPLRDS"
BASE COUNT          413 a          604 c          715 g          362 t
ORIGIN      
        1 gagagcgttg ttgaccagta ggagttcctg gtagcgctcg tagacccgcc gggtgatttc
       61 cgtgaagtac gtgtcggtcg tgagggctgc cggagcgatg agctcgttct tggctcgcga
      121 gatcgctccg agcacctggc ctggctggat ccgctgcggg tccaggttga ggtccttgag
      181 cgactggcgg acgagggcca gttgatctgc ctcgtcgaag ataacgaaat cccgactgac
      241 cggcagccgg tcggcttcaa ttcgcagcat gcgagcaaag aaggcgtgga aagtgcccag
      301 ccgaggttgg gctgcctggg tttgttgctg accgagcaag ctgccagccc gcttttccat
      361 ttcgcgggcg gctttgttgg tgaaggtcag ggcgacgatc tgctcgggcc ggatgccgag
      421 ttcctggatc aaataagcca atcgatgagt caacacccgg gtcttgccgg aaccaggtcc
      481 ggccaagacc aacgccggcc ccagcggggc agtcaccgct tcgcgttgct gggaattgag
      541 accgtggagc aagtccatac ggcgcctatt gtatcggtcg gagagcgggc tgtcctggat
      601 cgtcgagcgg ggaaagagag cggcttcagg aatctcgaag cgggggtctg gggctgagct
      661 ctaacaggtc ggaagatgcc agcagattgg tgagaggcat tgccgggaac tccatcaccc
      721 ggctgtcggc gaagtacggg aagtcgaagt ggatcggatc cccggccgac ttcaggggga
      781 ttggcatcag ccgagcggtg agcggcttgt tatgtcgcag cgcgagcgcg ccgagatcga
      841 tcagcagggc ggccagcgac tccttcgaga tatcacccgg caacgggatc gtgtcgaggc
      901 cgcaaccaca caaggtggac atcagcagca actcgctaag gcccagtcgg ccttcggcgg
      961 cccgcattgc cagaatcgag tcctccagga ccggcagcat caaaccgcag aagccggtgc
     1021 gggcgaactt ggcctggtcc agacaatccg cgaggaatgc ggctgccgcg gcagttccgg
     1081 atagacccag cgcaggagcc ccgagcagtt cgagcgcggt gcccaaggat cggtcgggct
     1141 caggataggg ggcgggcgag aagtcaactc ccaggaacct gacacttcga tctttggcaa
     1201 ccggttcggc ggcccgagtg agggccgcgg catgggtctc gatcgagctg accagctgcc
     1261 agcgcaccgt ggctagcgag gtggcgttcc ggaaactctc caccacgagg tcggcagact
     1321 cggttgcgag cgcgaccgcc ggcggcccgc cgtcatggta tgcagcgggg aagaacggcg
     1381 atcccgggcc gacgttggcc agccccgcga agcgcagatt ggcaaatcca ttgggagaca
     1441 ccgtggaagc ggcacgaatc gcctccgcgc aggcgcgggc ggccgggagg gagagtccgg
     1501 agggcaaact tgcatacagc ccggaacaaa acacgctctc cgccgcagcg agcacctcag
     1561 gcacgacccg gaagccctcc aactcctccg cgagggccgg gccgatattg gcgtactcga
     1621 tgccgtgagt cgcgcattcg gcagccagtt gcctggccaa ttccgggcgt tctcggggcg
     1681 agaccggttt gtcaatttgt aagggaggcg gggtagccag gcgcagagtc tgggtttcgt
     1741 agccggctcc gccgagggca tcccggatcg ctctcaaaca gtcggccatg gccgcgatcc
     1801 tcatcggctc taacggccag ccggggtcga ggaagccggt gatagcgcgg actttcatcg
     1861 gctatggagc ggtccgagca aggtacctgc agccttggtt ctgttcaagg cggaatgggc
     1921 aaatcgggtt gacacacggc cggtaagtcg tacatattct gtatacgaat gactacaaaa
     1981 agtatacgac aggattcaat ccttgaggca ctctttacat cgcaggcgcg gatcgccgtg
     2041 ctgaaactcc tgctgttgaa tgtgtccagc cggtactatc tccgcgaagt agca
//
LOCUS       DATDPQ010000208         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_606771, whole genome shotgun sequence.
ACCESSION   DATDPQ010000208 DATDPQ010000000
VERSION     DATDPQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606771"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="hutU"
                     /locus_tag="VI520_04800"
     CDS             <1..959
                     /gene="hutU"
                     /locus_tag="VI520_04800"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HLF37237.1"
                     /translation="ANAADIFAELVIRGVTPDVVTDQTPAHDPLSYVPAGMSLEAADR
                     LRITDPTAYTRASAQSMSRQVEAMLAFQARGAVVFDYGNNLRQRAFDAGVHSAFDYPG
                     FVPAYIRPLFCQGKGPFRWVALSGDPQDIYRTDHAIMELFPTDRPLHRWLRLAREKVK
                     FQGLPARICWLGYGERAAAGLRFNEMVASGELTAPIVIGRDHLDGGSVASPNRETEQM
                     RDGSDAISDWPLLNAMLNAVAGATWVSFHHGGGVGIGFSQHAGQVVVADGTQEAGRRL
                     ERVLTTDPGLAIVRHADAGYPEAIEAARRNGIQMPMLGSVEP"
     gene            1027..1098
                     /locus_tag="VI520_04805"
     tRNA            1027..1098
                     /locus_tag="VI520_04805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1059..1061,aa:Met,seq:cat)
     gene            1199..1642
                     /locus_tag="VI520_04810"
     CDS             1199..1642
                     /locus_tag="VI520_04810"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HLF37238.1"
                     /translation="MQFAGTVTINAPRPEVWKFLTDPHSVGLCAPGLESLEIIIPDKQ
                     FKAVAAVGFGSVSVKFNMNVEWLELDPPNRGKIKARGSAPGSAVEGSSEMILRDLPNG
                     STDIDWTADVLVFGTIASLASRLMGSLTRRLTGEFFNCVKAKIEG"
     gene            1654..2931
                     /locus_tag="VI520_04815"
     CDS             1654..2931
                     /locus_tag="VI520_04815"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLF37239.1"
                     /translation="MPGHLVANLLAFARLLRTVGIDAHPSGVMELVRALDLVGVKRRR
                     EVYSAARALLVTRYDQLALFDRAFELFWRQPGRRDGVTFEGFLPPVARTASPRVGESL
                     PLGLAAISLAPPIPPAPAEQEPEGIHAVRTYSPTELLRRKDFGSLSADELEEVKRLMS
                     ELVLDLGARRSRRWRQAGRYPTDLHYSIRRSLHSGGELFELFGRQPKWKPRALVVIAD
                     ISGSMERYSGLLLRFICGIARSQARSIESFVFSTQLTHITRHLGSRDVERAVAEITRN
                     VPDWSGGTRIGQSLRTFNFQWSRRVLGRRAVVLLISDGWDRGDNELLSQEMARLQRSC
                     HRLIWLNPLLGSPHFEPLTMGLQAALPFVDDFLPIHNLESLEQLAVELDQLSPNRPSR
                     RQPRPAFNTESGGAALSSRPSLAPYPHIRQSRN"
BASE COUNT          611 a          960 c          993 g          600 t
ORIGIN      
        1 tcgccaacgc ggccgacatc ttcgccgagc tcgtgattcg gggggtcacg ccggatgtgg
       61 tgacggacca gaccccggcc cacgacccgc tttcgtacgt cccggccggg atgagcttag
      121 aggcagctga ccggttgagg attacggatc cgacggccta cacgcgcgca agcgcccagt
      181 cgatgtcccg ccaggtcgag gcgatgctgg cctttcaggc gcgcggggcc gtggtgttcg
      241 attacggcaa taatctgcgc cagcgggcct tcgatgccgg cgttcatagt gcctttgatt
      301 atcccgggtt cgtgcctgcc tacattcggc cgctcttctg ccagggcaaa gggccgtttc
      361 gctgggtcgc cctctctggc gatccgcaag acatctaccg caccgaccac gcgatcatgg
      421 aactcttccc cacggaccgg cctctccacc gctggctaag gctcgcacgc gaaaaggtga
      481 aattccaggg gctgccggcc cgaatctgct ggctgggtta tggcgagcgg gccgcggccg
      541 ggctgagatt caatgagatg gtcgccagcg gggagctgac cgctccgatc gtaatcggtc
      601 gcgatcacct cgacgggggc tcagtggcta gtcctaatcg agaaaccgag cagatgcgag
      661 acggcagcga tgcgatctcc gattggccgc tgctcaacgc catgctgaat gcggtcgccg
      721 gggcgacttg ggtttcattt caccacgggg gtggggtcgg gatcggcttt tcccaacacg
      781 ccgggcaggt cgtcgtggcg gacggcaccc aggaggcggg ccgccgactc gagcgggtgc
      841 taacaaccga tccgggcctg gcgatcgtgc gccacgccga cgccggttat cccgaggcca
      901 tcgaggccgc ccgtcggaat gggatccaga tgccgatgct gggatcggtc gagccctgat
      961 ctgcattacc tcactcaaaa cccccgctga tataatcgcc ctcgccgccg cacctccagc
     1021 agacgtggcg aggtagctca atggcagagc aggggattca taagcccttg gttgggagtt
     1081 caaatctccc cctcgccacc tgatcatgaa gcccgattgg cttcgccgac caaatcagct
     1141 ggccgcaagc accgattcga agctgagccc ggaatgcacc tgatgagaaa gtaagcaatt
     1201 gcaattcgca ggtactgtga caatcaatgc cccgcggccg gaagtgtgga agttcctcac
     1261 cgatccacac tcggttggct tgtgcgctcc ggggctcgaa tcgctcgaaa tcatcatccc
     1321 ggacaagcag ttcaaggcag ttgccgcggt aggcttcggg tcggttagtg tcaagttcaa
     1381 catgaacgtc gagtggcttg aactcgatcc gccgaaccga ggcaaaataa aggcgcgggg
     1441 ttccgcccct ggaagtgcgg tcgaagggtc cagtgagatg atattgaggg atctgccgaa
     1501 tggttccacc gacatagact ggaccgcaga cgtgctggtg ttcgggacca tcgccagtct
     1561 ggcctcccgc ttgatgggaa gcctgacccg gcgactgacc ggcgagttct tcaactgcgt
     1621 caaagccaaa atcgagggat gaaggcaggg cagatgccag gccacctggt agccaacctg
     1681 ctggcgttcg cccgactgct gcgcactgtg ggcatagacg cccatccatc gggagtgatg
     1741 gaattggtgc gggctttgga cctggtcggc gtcaagcgaa gacgcgaagt gtattctgcg
     1801 gcccgcgctc tgctggtgac ccgttacgac cagctagcac tcttcgaccg ggcattcgag
     1861 ctattctggc gccaaccggg gcggcgcgac ggtgtcacgt tcgagggatt cctacctcca
     1921 gttgcgcgga cggcgtcgcc gagggtgggt gagtccttac cccttggcct ggcggcgatt
     1981 tcacttgccc cgccaatacc ccccgcgccg gccgagcaag agccggaagg gatccacgcc
     2041 gttcgtacct acagcccgac cgagctgctg cgccggaaag atttcggatc gctatcggcc
     2101 gacgagctcg aggaagtcaa acgattgatg tcggagctgg tattggatct cggagcccga
     2161 cgcagtcgcc gctggcgcca ggctggcagg tatccaacgg acctgcacta cagcatccgc
     2221 cggagcctcc actcgggagg agagttgttc gagctttttg ggcgccagcc gaagtggaaa
     2281 ccccgggccc tggtggttat cgccgacatc agcggatcga tggagcgata ctcaggcctg
     2341 ctcctgcgat tcatctgtgg gatagccagg agccaggctc gttcgatcga gtcctttgtg
     2401 ttcagcaccc aactgaccca catcacccgc cacctgggga gccgggatgt ggagcgggcc
     2461 gtggcggaaa tcacgcggaa cgtgccggat tggtccggcg gaacccgaat cggtcaatcg
     2521 ttgaggacgt ttaacttcca atggtcccgc cgggtacttg gcaggagagc cgtggtgctg
     2581 ctgatcagcg acggctggga ccgaggcgac aatgaattgc tgagccaaga gatggcccgc
     2641 ctccaacgca gctgccaccg cctgatttgg ttgaatcccc tgttgggatc tccccacttc
     2701 gagccgctta ccatggggct gcaggcagcc ttacccttcg tggatgattt cctgcccatt
     2761 cacaatctgg agagcttgga gcagctggcg gtcgagctcg atcagttgag cccgaatcgt
     2821 ccttcgaggc gccaaccgcg gccggccttc aataccgaga gcggaggggc ggccctaagc
     2881 tcccgcccgt cactcgcccc ctatcctcac atacggcaat ccagaaacta acggttgact
     2941 ctccccctcc ggagagatta gccttgccta aatattaatt ttggcataga atatattgct
     3001 ttgggccact gcccaattga gaaaggcggt ccgaatgacc gaagcaatcc ggctggaacg
     3061 gctgagcaag tcctacggaa aacaacgcgg agtggttgat ctggatttgg aagtccacgc
     3121 tggggaggtg tttggatatc taggccccaa tggcgcgggc aaaa
//
LOCUS       DATDPQ010000209         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1920488, whole genome shotgun sequence.
ACCESSION   DATDPQ010000209 DATDPQ010000000
VERSION     DATDPQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920488"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VI520_04820"
     CDS             <1..563
                     /locus_tag="VI520_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HLF37240.1"
                     /translation="QAIEVTESIGAFMRVSLLSGFALAFPYLCLEMFAFVNPGLKKNE
                     RRLVILAVPLATLLFLAGLAFTYYIVLPTALPFLLNFMDIPTTPRPLNYIRFVTNLML
                     WIGVAFEFPLLIYVLAGLGFVRARSLARSWRFAIVGIAVLAAAATPTVDPVNMALVMA
                     PMLVLYLVSIVLAAIAQAGRRRSQRL"
     gene            complement(551..1189)
                     /locus_tag="VI520_04825"
     CDS             complement(551..1189)
                     /locus_tag="VI520_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLF37241.1"
                     /translation="MPSILFTISMLSACGPPQAADEPAAPTQAAAGPYSLATPAAESA
                     ALETVGPATAEESDEAMALTVTSTGFEAGGVIPPKYSCDGEDISPPLAWSAPPPGTQS
                     FALIFDDPDAPGGTWVHWVLFNIPAAARSLSEGISPDPTLGDGSVHGTSSFNSLGYGG
                     PCPPGGTHRYFFKLYALDAILDLEGGATKADLEAAMETHVLAQAELRGTYSR"
     gene            complement(1214..2140)
                     /locus_tag="VI520_04830"
     CDS             complement(1214..2140)
                     /locus_tag="VI520_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HLF37242.1"
                     /translation="MSRLGAVVIGLLACGAAGLAGCSTLEAILPGQPAPTAPPLGFGL
                     SATQIKYSLIEVLGEPFYCDPDYYPVARADESELAQTWFDQVDKSSEEFQNILRQLDL
                     TGSTGLTSEQVLAAYREHKRLTAINLSPTGEGYRFEMRISEGEAGDLLVGTIDRAGRI
                     EIESKETSFNTCPICLDGDTRIATPAGNVAAKDLRVGMLVWTLDDSGARIVAPIVRTA
                     RVRVPVGHPMVHLLLEDGREVLASPGHPLADGQRLGRVLPGERLAGSEVVLAEWIEWE
                     ETYTYDILPSSETGLYWANGILLRSTLSPAGR"
     gene            2198..3562
                     /locus_tag="VI520_04835"
     CDS             2198..3562
                     /locus_tag="VI520_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37243.1"
                     /translation="MRPVYAISGGVSKFAKARPDKTFQAVVKEAYDYAFRDLGLDFPA
                     FTAQVDGSVASYFSDHFSRQLMSGIMAQDYLGLCPKPSHRVEGGGATGGLCFQEAWKS
                     VASGHMDVCVAYGFETMSHVETWKGNEFIALASDVTWDYPVGGFYSGYYAMMVTRHMK
                     EYGTTVEQMAAVSVKNHINAYYNPYAQKRNRYTIEDVRNSPMVAWPLTRLDICVMSDG
                     AAVAVLASEEGLRRLERAGAVIRRPLVQVTGIGRGTDAMRMSDRPHLDYDTFLREYAT
                     EQEKESDDTRTYYRKLWDHGTRYPGVHSFRAGRTAGNMAYKHAGIRDPLNELDFIELH
                     DAYTSSELQTYEDLGLCRYGEGGSFAVSGKAFMPGIDYGLELKDLPVCPVNPSGGLIA
                     CGHPVGATGLMQAVFALWQLQARVGAHFQDETLQLKDARRGAIHSHAGTGTYVTVTIL
                     ERAE"
BASE COUNT          658 a         1155 c         1115 g          698 t
ORIGIN      
        1 tgcaggcaat tgaagtcacc gaatcgatcg gggccttcat gcgggtctcg ctgctgagcg
       61 gatttgccct ggcgttcccg tacctctgct tggagatgtt cgccttcgtg aatccggggc
      121 tgaagaagaa cgaacggcgg ctggtgatcc tggccgtgcc gttggccacc ttgttgtttc
      181 tggccgggct ggcgttcacc tactacatcg tgctgccgac tgcgctgccc tttctgctca
      241 atttcatgga cattcccacc acgcctcgcc cgttgaacta catccgcttc gtgaccaacc
      301 tgatgttgtg gatcggggtg gcattcgagt ttccgctttt gatctatgtg ttggccgggc
      361 tgggcttcgt acgggcccgc tcgctggccc gctcatggcg cttcgccatc gtcggaatcg
      421 cggttctggc ggccgccgca accccgaccg tcgacccggt aaacatggcg ctggtcatgg
      481 ccccgatgct ggtcttgtac ctggtaagca ttgtattggc ggccatcgcc caagccgggc
      541 ggcggcggtc tcagcggctg taggtccccc tcagctccgc ctgggccaac acgtgagtct
      601 ccatggccgc ctccaggtcg gctttggtgg cgcccccttc cagatcaagg atcgcatcca
      661 acgcgtacag cttgaagaaa tagcggtgcg taccacccgg tgggcacggc cctccgtagc
      721 ccagcgaatt gaagctgctg gtgccgtgga cgctcccatc ccccagagtc gggtcgggcg
      781 agatgccctc ggagagtgac cgagcagccg ctggaatgtt gaacagcacc cagtgcaccc
      841 aggtgccacc cggcgcatcg gggtcatcaa agatcagcgc gaagctctga gtcccgggcg
      901 ggggggcgct ccaggccagc ggcggagaga tgtcctcccc atcgcatgag tacttcggag
      961 ggatcactcc cccagcctcg aacccagtgc tcgtaacggt gagggccatt gcttcatcac
     1021 tctcctccgc tgtcgccggc ccgacagtct cgagtgccgc actctctgca gcgggggtcg
     1081 ccagcgagta gggtccggct gccgcttggg tcggggcagc cggttcatcc gcggcctgtg
     1141 gaggaccgca ggcgctcagc atgctgatcg tgaacaggat cgacggcaag caaatccggc
     1201 tccatttcat aggtcaccgc cctgcgggag ataacgtgct gcgaagcaga attccattcg
     1261 cccagtacag gccagtctcg ctcgatggga gaatgtcgta ggtgtaggtt tcctcccact
     1321 cgatccactc cgccaggaca acctccgagc ctgccaggcg ttcaccgggg aggaccctac
     1381 cgagtcgctg cccgtccgcg agcgggtggc ccggggatgc gagcacttcg cggccgtctt
     1441 cgagcagcag gtgaaccatc gggtggccga ccgggacgcg gacccgagcc gtgcgcacaa
     1501 tgggtgctac gattcgggca cccgaatcgt ccagggtcca gaccaacatt ccaactctca
     1561 agtctttcgc cgccacattc cctgctgggg tagcgatccg ggtgtcgccg tccagacaga
     1621 tcggacatgt gttgaaactt gtttccttgc tctcaatctc gatccgaccc gctcgatcga
     1681 tagtgccgac cagcaggtcg cccgcctcgc cttcggagat ccgcatctca aaccgatacc
     1741 cttcacccgt cgggctcagg ttgatcgcgg tcagtcgctt gtgttcccga taggcagcga
     1801 gcacctgctc gctcgtcagc ccggttgagc cggtgaggtc gagctgccgc aggatgttct
     1861 gaaactcctc gctgctctta tccacttggt cgaaccacgt ttgggccagt tcgctctcat
     1921 ccgcacgggc tacggggtag taatcggggt cgcagtagaa gggttccccc agcacctcga
     1981 tgagcgaata cttgatctgg gtggctgaca ggccgaaacc aagcggcggc gccgtcggcg
     2041 ctggctgccc gggcaagatc gcctcgagcg tgctgcagcc ggccaacccg gcagccccgc
     2101 aggccagcaa gccgatcacg accgcgccca gtcggctcat cgtccgaacc cagccgaagt
     2161 acaatcccca ctcagcgata gagtcaatga ggagctaatg cgcccagtct acgccatatc
     2221 gggcggggtc tccaagttcg ccaaggcccg cccggacaag acctttcaag cggtcgtcaa
     2281 ggaagcttac gattatgcct tccgggacct cggcctggat ttcccagctt tcaccgccca
     2341 ggtggatggc tcggtcgcct cctacttctc cgaccatttc agccgccagc tcatgtcggg
     2401 gatcatggct caggattacc tcggcctgtg ccccaagccc agccaccggg tagaaggcgg
     2461 gggcgccacc ggaggtttgt gttttcagga agcctggaaa tcggtggcct ccggtcatat
     2521 ggacgtctgc gtggcgtacg gattcgagac catgagccat gttgaaacct ggaagggcaa
     2581 cgagttcatc gccctggcct ccgacgtgac ctgggattac ccggtcggcg gattctactc
     2641 gggctattac gccatgatgg tcacccgcca catgaaggag tacggcacca ccgtcgagca
     2701 gatggcggcg gtctcggtaa agaatcacat caacgcctac tacaaccctt acgcccagaa
     2761 gcgcaaccgg tacacgatcg aggatgtgcg caattcgccc atggtggctt ggccgttgac
     2821 ccggctcgat atatgcgtga tgtcggatgg ggccgcagtt gcggtgctgg cctcagaaga
     2881 gggacttcgg cggctggagc gggccggggc ggtcattcgc cgcccattgg tgcaggtcac
     2941 cgggatcggt cgtgggaccg acgccatgcg gatgtccgac cgtccccacc tggactatga
     3001 caccttcctg cgcgaatacg ccaccgaaca agaaaaggaa tccgacgata cccggaccta
     3061 ctaccgcaaa ctctgggatc acggaacccg ctatcccgga gtgcactctt tccgggcggg
     3121 gcggaccgcc ggcaatatgg catacaagca tgccggcatc cgcgatccgc tcaatgagct
     3181 ggactttatc gagctgcatg atgcctacac ctcaagcgag ctccagacct acgaggacct
     3241 gggactgtgc cgttacggtg agggcgggag ctttgcggtt tcaggcaagg cctttatgcc
     3301 cggaattgat tacggactgg aattgaagga cctcccggtc tgcccggtca acccgtccgg
     3361 cgggttaatc gcctgcggac acccggttgg ggcgacggga ttgatgcaag cggtattcgc
     3421 gctctggcag ctacaagcgc gagtcggcgc ccacttccag gatgaaacgt tgcaactcaa
     3481 ggatgcccgc cggggagcga tccactcgca tgccggcacc gggacctacg tgacggttac
     3541 gatcctcgag cgtgcggagt gagcccgggt ccagcgataa gccgggtcat attgtgtaga
     3601 aattcacaaa ggaggcctga ggcgta
//
LOCUS       DATDPQ010000210         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2198522, whole genome shotgun sequence.
ACCESSION   DATDPQ010000210 DATDPQ010000000
VERSION     DATDPQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198522"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VI520_04840"
     CDS             complement(<1..590)
                     /locus_tag="VI520_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLF37244.1"
                     /translation="MRRKPIYLDYAATTPLDPRARMGMLPFFDEAYGNPASVHSWGQQ
                     AEAALEQSRARIAANLGCGTDEIVFTSGGSESDNLALRGAAFAARQGRSANHILISPV
                     EHPAVLETGRQLQQLHGFELEYLPVDHYGRVEPGDLGERLRSDTAVVSVIYGNNEIGS
                     VNPISELGILCQQQGIPFHTDALQAVAQLELRVDELP"
     gene            669..1973
                     /gene="eno"
                     /locus_tag="VI520_04845"
     CDS             669..1973
                     /gene="eno"
                     /locus_tag="VI520_04845"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLF37245.1"
                     /translation="MDTRIAGVDALEQLDSRGNPTVAARVNLRDGSWGEALVPSGAST
                     GLHEALELRDGDPNRYLGKGVRKAVSHIREEIAERIIGLTAEDQPALDHVLIELDGTP
                     NKARLGANAILAVSLAAAKAVAAARGLSLFRSLGGEQAVTLPVPMLNILNGGAHTGWQ
                     TTDAQEFMIMPLGAPTFSEAMQWGVEIYHALRAVLKRKGFAVLVGDEGGYAPALKANV
                     EAVEVILEAVEQAGYRAGEQVGLALDPAASEFYSEGTYRLRREGRVLDSGGMVEYWAD
                     WVRQYPIVSLEDGLAQDDWEGWKAMTRELGDQIQLVGDDLLVTNPTRVRIAIDGRACN
                     ALLVKVNQIGTLSEAVEAVQLCREVGWATVASHRSGETEDSTIADLCVGLSMPQIKTG
                     APARSDRVAKYNRLLWIEHELGSAAKYAGKAALRQPWPRRTN"
     gene            complement(1966..2436)
                     /locus_tag="VI520_04850"
     CDS             complement(1966..2436)
                     /locus_tag="VI520_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLF37246.1"
                     /translation="MNLSSATTAFQYLTALFGAFLAASWLSLIFWTYRDVRSRTHDPL
                     VQILSALTTGVMGPFGLFIYLILRPSQTIDQAYQHTLEEEALLTEIEEQPICPGCGNR
                     TRAEWQLCPNCHTHLRKGCEHCGRLLELSWQICPYCGTPVPGVRAEVGLDPGAS"
     gene            2511..>3161
                     /gene="radC"
                     /locus_tag="VI520_04855"
     CDS             2511..>3161
                     /gene="radC"
                     /locus_tag="VI520_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLF37247.1"
                     /translation="MTETGKTYALHRITDLGPADRPRERLRDQGAEVLHNAELLAILL
                     RTGVAGLNSVQLAQELLMKFHGLAGLHDANYAELVASRGIGDAKAAQIKAAIELGRRL
                     AAWKPEQRPSIQSPEHAAELLMYEMSTLDQEHLKVLLLDTRNQLLRVVEVYHGSLNTS
                     LVRISEVFRDAVRDNAAAIIVVHNHPSGDPSPSPEDIAVTRAIVEAGKLLDIEVLDH"
BASE COUNT          613 a          918 c         1057 g          573 t
ORIGIN      
        1 gggagctcat cgacccgcag ctcgagctgg gcgacggcct gcagcgcgtc ggtgtgaaac
       61 gggatgccct gctgctggca gaggatcccg agttcggaga tcggattgac cgaaccgatc
      121 tcgttgttgc cgtagatcac cgaaacgacg gctgtgtcgg atctcaatcg ctcgccaaga
      181 tcacccggct ccactcggcc gtagtggtcc accggtaggt attccaactc gaagccgtgc
      241 agctgttgca gctgcctgcc ggtttcgagc accgcgggat gctcgaccgg gctgatcagg
      301 atgtggttgg cggagcgccc ttgccgagca gcgaaggctg cccctcgaag cgcaaggtta
      361 tcgctttcgg atccgccgct ggtaaataca atctcatccg tcccgcaacc cagattggcg
      421 gctatccgtg cccgggattg ctccagggcc gcctccgcct gctggcccca ggagtggacg
      481 gaagccggat tgccgtaggc ctcatcgaag aaaggcagca tgcccattcg cgcccgtgga
      541 tccaagggcg tggtcgccgc gtagtcgaga tagattggct tccggcgcat tgggcgattg
      601 taacattctc atgctccgtg gctctccgcc tgggttgcgc ctccgggctg gttataatcc
      661 gctacccaat ggacacgcga atcgctggtg ttgatgcctt ggagcagctc gactcgcggg
      721 gcaaccccac ggtcgcagcc cgagttaact tgcgcgacgg tagttggggc gaggccctgg
      781 ttccctccgg agcctcgacc ggactacatg aggcgctaga gctgcgtgac ggggacccga
      841 atcgctacct cgggaagggt gtgcggaagg cggtcagcca catccgagaa gagattgccg
      901 aacggattat cggattgacg gcggaggatc aaccagcgct cgatcacgtg ctgatcgagt
      961 tggatgggac accgaacaag gccaggcttg gcgcgaatgc gatcctggcc gtcagtctgg
     1021 ccgctgccaa ggcggtcgcc gcggcccgtg gcttgtcact tttccgctca ttggggggtg
     1081 aacaggcggt cactctgcca gtcccgatgc tcaacattct caatggtggg gcgcacactg
     1141 gatggcagac caccgacgcc caggagttca tgatcatgcc gctgggcgcg ccgaccttct
     1201 ccgaggcgat gcaatggggg gtcgagattt accatgcgct gcgggcggtg ctcaagcgga
     1261 aggggttcgc cgtgctggtc ggggacgagg gaggctacgc cccggccctg aaagccaatg
     1321 ttgaggcagt cgaggtgatc ctggaggccg ttgagcaggc cggctaccgg gccggcgagc
     1381 aagtgggcct ggcgctcgat ccggctgctt ctgaattcta cagtgagggc acctaccgcc
     1441 tgcgtcgaga ggggagggta ctcgactcgg gggggatggt ggaatactgg gccgactggg
     1501 tcaggcagta tccaattgtc tcactggaag acggtctggc tcaggatgat tgggaaggct
     1561 ggaaggccat gacgcgtgag ctgggggacc agatccaact ggtcggggac gatctgctgg
     1621 ttaccaaccc gacccgcgtg cgcatagcca ttgatggtcg ggcctgcaat gccctgttgg
     1681 tcaaagtaaa tcagatcggg accctcagcg aagccgttga ggcggtccaa ctctgccggg
     1741 aggttggatg ggcgaccgtg gcctcccatc gctcgggtga gacggaggac tcgacgatcg
     1801 ctgacctgtg cgtaggattg tcgatgccgc agatcaagac cggcgcgccg gcccgctccg
     1861 accgggtggc aaaatacaac cgactgctgt ggattgagca cgaattggga tcggctgcga
     1921 aatatgcggg gaaggcggcg ctgaggcagc cctggccgag gcgaactaac tagctccagg
     1981 gtcgagcccc acctcagccc gcacaccggg aacgggtgtt ccgcagtagg gacagatctg
     2041 ccaggagagc tccagcaacc ggccacagtg ctcacagccc ttccgcaagt gggtgtggca
     2101 gttcgggcag agctgccatt cggcccgggt tcgatttcca cagcctgggc agatcggctg
     2161 ctcttcaatc tccgtcaaga gcgcttcctc ttccaaggtg tgctggtagg cctgatcgat
     2221 cgtctgggac ggtcgcagga tgaggtagat gaacaggccg aatggcccca tgaccccggt
     2281 cgtcaaggca gacagtattt ggacaagggg atcgtgagtt ctggaacgga catcgcggta
     2341 ggtccagaag atcaagctca gccaggacgc agccaggaac gccccaaaca aggcggtcag
     2401 gtactgaaat gcggtggttg cggagcttag attcaccagg gccctgaggg gtctgcgtga
     2461 ttatacagtg ggttgcgcaa tccaggcctt aggccgaggg aggctggggg atgacagaaa
     2521 cgggcaagac ctacgcgcta caccggatta ccgacctcgg tccagcggat cggccgcgcg
     2581 agcggctgcg cgatcagggg gcagaggtcc ttcacaacgc cgagctgctc gccatcctgc
     2641 tcagaactgg ggtggccggc ctcaactctg ttcaactggc ccaagaactc ctgatgaagt
     2701 tccacggtct ggccgggctg cacgatgcca actacgcaga gctggtggcg agtcgaggaa
     2761 ttggagacgc caaggcagct cagatcaagg ccgccatcga gctggggagg cgtctggcgg
     2821 catggaaacc ggagcagcgc ccctcgatcc aatcacccga gcacgcggcc gagctgctga
     2881 tgtatgagat gagcaccctc gaccaagagc acctcaaggt cctgttgctt gatactcgca
     2941 atcaactgtt gcgggtggtt gaggtatacc atggctcgct aaacacttct ctagtccgga
     3001 tcagcgaagt gttccgcgac gcagtccgcg acaatgccgc cgcgattatc gtggtccata
     3061 atcaccccag cggcgatccg tctccgagcc ccgaggatat tgcggtcacc cgggccatcg
     3121 tggaagccgg caagctgctg gatatcgaag tgctggatca c
//
LOCUS       DATDPQ010000211         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2198720, whole genome shotgun sequence.
ACCESSION   DATDPQ010000211 DATDPQ010000000
VERSION     DATDPQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198720"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..2341)
                     /locus_tag="VI520_04860"
     CDS             complement(191..2341)
                     /locus_tag="VI520_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37248.1"
                     /translation="MDQSNDSMLVQAIAAARSGNRVRARSLLTRLLKADSGVVEYWIW
                     MSSTVDTKREKIYCLESALKLDPTNRAVLRGLTILGVRPPLDSELASAVKIARRQVPA
                     SISSGPAVGRALRLNWRFIATGFAGVVAVLLLVTGVYAFVTRPRGLARAPTLPPEGAE
                     ASATAPVPTATKTPVPPSISIIRTAIPTELALTPILFFVPQTPTPTPIYGLTPHPVYE
                     AYSSGVSALTRGDLADAVAFFDQVIELDDALPDVHFFRAEALRMLAAAESGSEVGSLL
                     GQAIASYDRAILLDPEFAPAYLGRGHALLQRTVLRIGQPDDLKAEDLPADYAKAFEVD
                     PSFSPAYAAQAEFLRGVRLWKTMEEVLQAAVDRGVREPLLYILLSEAQFNGDKVDLAL
                     ENAVEGSAGDHTNLDGYLMLGRALVWLEQYPLAIDPLLTYLDYRGEDHRGWADLGRAQ
                     LEVGDLPAASSSLNRALSLNERYAPAYLSRGWLNLQVGAYETGLSDFNDARRYGPETI
                     EILLGIGNAYYYLGELQQALESANTAIELASQDQRQELRERDTSRGYELRARTAEVAT
                     DLLDYAIQNYHWILELQMPDPEMRAIAEQRLLVLTGEIPTAVPTLSVSPTPSPSATTI
                     GTVSATPTPSSTPIPSLTPTPTMTQTPSRTPTPSRTPTPSRTPTPSRTPTPSRTPTPT
                     RTPTTTISPTPGTVTPSPTPSGSTPTKTPTPPFA"
BASE COUNT          459 a          787 c          873 g          460 t
ORIGIN      
        1 cgggagtgat cgaacaggtc gccggcgatg atcaaaccct cgacgcccag ctcgccgcac
       61 tgccgcagga tgtcctccag cgcggcgaat cgctcggggt gctctttttg ggtcgtcagg
      121 tggagatcgg ccgtcatggc gatgcgcatg gcacccccaa tcctatcgca atcgcgccgg
      181 cctcgagcgc ttacgcgaag ggcggtgtcg gcgtcttggt gggagttgag cccgaggggg
      241 ttggagaggg agtgactgtc ccgggcgtcg gcgagatggt ggtcgtcggc gtgcgggtgg
      301 gggtgggtgt ccggctcggg gtgggtgtcc ggctcggggt gggtgtccga ctcggggtcg
      361 gggttcgact gggagtcgga gttcgactcg gagtttgagt catggtcgga gtcggtgtga
      421 ggctggggat cggcgtggag ctgggcgtcg gcgtagcgct gacggtgccg atggttgtcg
      481 cgctcgggct tggcgtcgga gatacgctca aggttgggac tgccgtcggg atctcgccgg
      541 taagcacaag cagccgttgt tcggcaatcg cgcgcatttc aggatccggc atctgcagct
      601 ccagaatcca atgatagttt tggatcgcat aatccagcag atccgtagcg acctctgctg
      661 tacgagcgcg cagttcatac ccgcgagaag tgtcccgctc gcgaagctcc tggcgctggt
      721 cctgcgaagc cagctcaatc gcggtgttgg cgctttcgag agcctgctgc aattcgccga
      781 gatagtagta ggcgttgcca atccccagaa gaatctcgat cgtctccggt ccatagcgcc
      841 gagcgtcgtt gaaatcggaa agcccggtct cataggcacc gacctggagg tttagccaac
      901 cgcgggacag gtacgccggc gcgtaccgct cattgagact caaggcccga ttgagcgaag
      961 aagaggcagc cggtaagtcg ccaacttcca actgcgcccg tccgaggtcg gcccagccgc
     1021 gatggtcctc gccccgataa tcgaggtagg tgagcagcgg atcgatggcc agcgggtatt
     1081 gctcgagcca gaccaaggct cggcccagca tgagataccc gtccaagttc gtgtggtcgc
     1141 cggcgctccc ttcaactgcg ttctcaaggg ccagatcgac cttgtcgccg ttgaactgtg
     1201 cctcgctgag gagaatgtaa agcaggggct cgcgcacacc tcgatcaacc gcagcctgga
     1261 ggacttcttc catggtcttc cagaggcgca ccccccgcag gaactccgcc tgggccgcgt
     1321 aggcgggcga gaaactcgga tcaacctcga aggccttcgc gtagtcggcc gggaggtcct
     1381 cggctttcag gtcgtccggt tgcccgatcc gcagcactgt gcgctgcagc agggcgtggc
     1441 cacgccccag gtatgccggc gcgaactcag ggtccaacag gatcgcccgg tcataactgg
     1501 cgattgcctg gccgagcagg ctgccgactt cagaccccga ctcagccgcg gccaacatcc
     1561 gaagcgcctc ggctctgaag aagtgaacat ccggcagagc gtcgtccaac tcaatcacct
     1621 ggtcgaagaa cgctaccgcg tccgccaggt cgccgcgggt cagcgcggac accccggacg
     1681 aatacgcttc gtataccgga tggggggtca gtccgtagat cggggttggg gtcggagttt
     1741 ggggaacgaa gaacaggatg ggagttagcg ccaactcggt cgggatcgcg gttcggatga
     1801 tgctgatcga cggcgggacc ggggtcttgg tcgcggtcgg gacgggggcg gtcgcgctgg
     1861 cctccgctcc ctcgggaggc agggtcgggg cccgagccag ccctcgcggc cgggtgacaa
     1921 atgcatacac cccggtgacg agcaacagga ctgcaaccac ccccgcgaac ccagtcgcaa
     1981 tgaaccgcca attcagtcga agcgcccggc cgaccgccgg cccgctggaa atggaagcag
     2041 gaacctggcg ccgagcgatc ttgactgccg aggccagctc cgaatcgagc ggcggtctga
     2101 cccccagaat tgtgagaccg cggagcactg cccgattggt ggggtcaagt ttgagggccg
     2161 actcgaggca gtagatcttc tcccgcttgg tgtcgacggt cgagctcatc caaatccagt
     2221 actccacgac tccggagtcc gctttgagca ggcgagtcaa caggcttctt gcgcgcaccc
     2281 ggttcccgga acgggctgcg gcaatagcct gcacgagcat gctgtcgttg gattgatcca
     2341 tgccagctga aggagccgaa gtctagcata gcggtttggc cggcacaacg cgacctcggt
     2401 acgccccctt gctataatcc ccacccgata tgtgccggat gttgctggcg atttgggggg
     2461 tcatcctgtg gggctgcgtg ccgatcagcc gggcgacccc tgagctgccc actcaaactc
     2521 tccaacctcg ccttgagcgc cagaacaccg cgaccgagcc cgctccggcg acagtcagc
//
LOCUS       DATDPQ010000212         3578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3766164, whole genome shotgun sequence.
ACCESSION   DATDPQ010000212 DATDPQ010000000
VERSION     DATDPQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766164"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VI520_04865"
     CDS             complement(<1..279)
                     /locus_tag="VI520_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37249.1"
                     /translation="MYSAITAEGPSEAQRRELRLLSVAFTFLVLAALAMAIPRATSGE
                     AASLQLGHLVFLPIWAGCAWMMRRFADRGNSRRDPLLLPIVLLLAGWGV"
     gene            complement(257..1120)
                     /gene="mtnP"
                     /locus_tag="VI520_04870"
     CDS             complement(257..1120)
                     /gene="mtnP"
                     /locus_tag="VI520_04870"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HLF37250.1"
                     /translation="MTEARIGVIGGSGLYQMAGLEVEETRTVSTPFGEPSAPIVLGRF
                     NGKPIAFLARHGRHHQISPSEVNYRANIFALKLLGVERVISVSACGSLREDYAPGHVV
                     VPDQLLDFTRLRPRTFFEGGIVAHVSVADPFCPVVSQALVESVSAAGGIVHPRGSFLT
                     IEGPRFSTRAESNTFRGWGVAIIGMTTSPEAFLAREAEMCYAVLAHVTDYDVWHRSEA
                     PVTVEMVIRTLEANTRLAEQALADAVVRLPAVRDCICKDALKDALITVPDAIPAETLE
                     KLRPLVQRYYG"
     gene            complement(1129..1575)
                     /locus_tag="VI520_04875"
     CDS             complement(1129..1575)
                     /locus_tag="VI520_04875"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF37251.1"
                     /translation="MNPELALLALRLGLALVLYTFFGAVLLFLWRDLRASARASEIAP
                     AAHLELRTDTASSRGVPLSNLNLVGRASGNTILIDDGTVSSHHARLSYQGGQWWLEDL
                     GSKNGTRVNELDVEDPIVVTFGDQIRFGNVMTELKSGPAPHKDGEG"
     gene            complement(1572..2393)
                     /locus_tag="VI520_04880"
     CDS             complement(1572..2393)
                     /locus_tag="VI520_04880"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HLF37252.1"
                     /translation="MLEPLAKLESQLEKLFEGSLARLLREELSTATLASQLARTMVDG
                     VRRDEAGNAYAPNEFALTLNPDDVAKVLEQAPGIQATLSTGLSEVARQCGYLMAQEPN
                     ITMASDPTLNRRELRVISWHSTDPLEFTKAMVREPSATGSKLPKGAFLILDDGSHFAL
                     DRPVINVGRRIDNQIILENEHVSRTHAQMRIRDGRYVVFDLGSTSGTKVNGRKVKQHI
                     LRAGDVITIAGLRMVYGEDPGGPPDTATAYTPPFPPRPAGDQRTVTGEWDSEEGG"
     gene            complement(2503..3366)
                     /locus_tag="VI520_04885"
     CDS             complement(2503..3366)
                     /locus_tag="VI520_04885"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HLF37253.1"
                     /translation="MEEPAELLVEHLQSFVKELLVALPQGKFPLDETATHWAREIAAS
                     AKSDPGGRRFAPDQYTLSMHPKDYAALTEADHLSQTRLSKALQSALEHSKYHLSREPH
                     ITLATDPMLDRWQLRVIAWHSSDPTEAPPIVEEPANETPPKGAFFMVEGSRHFPLDRP
                     LINIGRRLDNQLVLEDPHISRRHAQLRARGHRYVLYDLNSTSGCRVNGKQVQEWVLRP
                     GDVVTLGTVQLIYGEDAKGPPDVTPPYSPPFKPEIDRDRVTPLNLSTIELNDNPVPTR
                     ELGSDPSKPRK"
BASE COUNT          665 a         1082 c         1161 g          670 t
ORIGIN      
        1 gactccccag cctgccaata gcagcacgat cggcagcagc agcggatcgc gcctcgagtt
       61 gccccggtcg gcgaatctcc gcatcatcca ggcgcagccg gcccagatcg gcaggaacac
      121 caggtgcccc agctggagtg acgcggcctc tccggatgtc gcccgaggaa tggccatcgc
      181 cagtgcggcg agcaccagga aggtgaaggc gaccgacagc aaacgcagct cgcgccgctg
      241 agcttcggac ggtccctcag ccgtaatagc gctgtacaag gggtctcagt ttctcgaggg
      301 tttcagccgg gatcgcgtcg ggaaccgtaa ttagggcatc tttgagggcg tccttgcaga
      361 tgcaatcacg cacggctggc aaacgaacca ccgcgtctgc cagagcctgt tcggctagcc
      421 gggtgttggc ttctaaggtt cgaatgacca tctcgacggt caccggcgcc tctgatcggt
      481 gccagacatc gtagtcggtg acatgggcca ggaccgcgta acacatttcc gcttcacgag
      541 ccagaaatgc ttcgggggag gtagtcatgc caatgatcgc gacgccccag ccgcggaatg
      601 tgttcgactc ggcccgggtg gaaaaacgag gcccttcgat cgtaaggaat gagccccgcg
      661 ggtgcacaat cccacccgcg gccgaaacgc tctccaccag ggcttgggag acaaccgggc
      721 aaaacggatc ggcgacgctg acatgcgcga cgatcccccc ttcgaagaag gtccgaggac
      781 gcaagcgcgt gaagtccagc agctggtcgg gcaccaccac gtgcccgggg gcgtagtcct
      841 cgcgtaacga tccacaagca ctgaccgaga tcacccgctc gactccgagc aacttgaggg
      901 cgaatatgtt ggcccggtaa ttcacttcgc tcggcgagat ctgatgatga cggccgtgcc
      961 tggcaaggaa agcgatcggc ttgccgttga aacggccaag gacgatcggc gcgctgggtt
     1021 ccccgaatgg ggtactgacg gttcgggtct cttcgacctc cagaccagcc atctggtaca
     1081 ggccggaacc tcctatcacc ccgatccggg cttcagtcat cgacgcgatc agccctctcc
     1141 gtccttatgg ggggccggcc cactcttcaa ctcagtcatc acattgccga accgaatctg
     1201 gtcaccgaag gtcaccacta tgggatcctc gacatccaat tcgttgaccc tggtcccgtt
     1261 cttcgaaccg agatcctcca gccaccactg accgccttgg taagacaggc gggcgtggtg
     1321 agaggataca gtgccatcgt cgatcaggat cgtgttgccg ctggcccggc cgaccaggtt
     1381 cagattggaa agcgggaccc cgcggctgct cgcagtgtcg gtacggagtt ccaggtgggc
     1441 agccggggcg atttctgagg ctcgggcgga agcgcgcaga tcacgccaga ggaagagcag
     1501 gactgcgccg aagaaggtat acagtaccag ggccagcccg aggcgcaggg ccagcagggc
     1561 gagctcgggg ttcatccacc ctcttcgcta tcccattcgc cggtgacggt tcgctgatcg
     1621 cctgccggcc gtggtggaaa aggcggggta taggcggtgg ccgtgtccgg cggacccccc
     1681 ggatcttcgc cgtagaccat ccgaagtcca gcgatagtga tcacgtctcc ggcccgcagg
     1741 atgtgctgtt tgaccttgcg gccattgacc ttggtgccgc tggtcgagcc caggtcgaat
     1801 acgacgtagc ggccgtcccg gatccgcatc tgggcatggg tacgggaaac atgctcgttt
     1861 tccaggatga tctggttgtc aatcctgcgc ccgacgttga tgaccggccg gtcgagggcg
     1921 aaatggctgc catcgtccag gatcaagaag gctcctttgg gtagcttgct gcctgtggcc
     1981 gagggctccc gcaccatggc cttggtgaat tcgagcgggt cggtgctgtg ccacgagatg
     2041 acccgcagct cccttcgatt caaggtcgga tccgaggcca tggtgatatt cggttcctgg
     2101 gccatcaggt agccgcactg cctggccacc tcactgagtc cggtcgagag ggtcgcctgg
     2161 atacccggtg cctgctcgag gactttcgcc acatcatcgg ggttcagggt gagggcgaac
     2221 tcgttggggg cgtaggcatt gccggcctcg tccctgcgca cgccgtccac catggtccga
     2281 gcaagctggc tggccagggt ggcggtagac agttcctccc gcaacaacct ggccagcgag
     2341 ccttcgaaga gcttctcgag ctgactttcg agcttggcaa gcggctcaag catcgggcga
     2401 gattataacc agaaacggac gcctccctcc caactggcag gccagtgggt aggacgcaga
     2461 gttgcccgat ggttgcatgc cgaccgatcc aggagatcgg ggttatttgc gaggcttaga
     2521 ggggtctgag cccagttccc tggtcggaac cgggttgtca ttgagctcga tcgtggacag
     2581 gttgagcgga gtgactcgat ctcgatcgat ttccggctta aagggcggcg agtagggagg
     2641 agtgacatcg gggggaccct tggcatcctc accgtagatc aactgcacgg tgcccagggt
     2701 cacgacgtcg cctggtcgca gcacccactc ctgaacctgt ttgccgttca ctcggcagcc
     2761 ggaagtggaa ttgagatcgt agagcacgta gcggtggcct cgcgcccgca gttgagcgtg
     2821 ccgccgggaa atgtgcggat cttctaagac gagctggttg tccaaacggc ggccgatgtt
     2881 gatcaatggc cgatcgagcg ggaaatggcg gctgccttcc accatgaaga acgccccctt
     2941 cggtggagtt tcattggcag gttcctccac aatcgggggc gcctcggtgg gatcgctgct
     3001 gtgccaggcg atcacccgca gctgccagcg gtccagcatg ggatcggtcg cgagggtgat
     3061 atgcggctct cgcgagagat ggtatttgga gtgctctaac gccgactgca acgccttgga
     3121 aagcctggtt tgagacagat ggtctgcctc ggtcagcgcc gcatagtcct ttgggtgcat
     3181 gctgagggtg tactggtcgg gggcgaagcg tctcccgcct gggtccgatt tggcgcttgc
     3241 ggcaatctcg cgggcccagt gggtcgcggt ctcatccagc gggaacttcc cctggggcag
     3301 ggccaccagc agctctttta caaagctctg caggtgctcc acgagcagct ccgcgggctc
     3361 ttccactacc ggcattatac gccgactacc acgccttaca accacttcat cttcctaggc
     3421 gctgcgccca ggcctgcggg agggctggga tatactgacc ctatgccgtc gggcggtttt
     3481 gaggagctgg agcacaccgc cgactgggcg ctcagagtac gcgggcggga tctggccgag
     3541 ctgctggtga acgcggcgaa cggaatgatg gagctggc
//
LOCUS       DATDPQ010000213         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_96219, whole genome shotgun sequence.
ACCESSION   DATDPQ010000213 DATDPQ010000000
VERSION     DATDPQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96219"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1440)
                     /gene="dnaA"
                     /locus_tag="VI520_04890"
     CDS             complement(136..1440)
                     /gene="dnaA"
                     /locus_tag="VI520_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HLF37254.1"
                     /translation="MNAERLWQAALGQLQVEMPKAAFDTWVRDAELLAYEDGQFIVGV
                     QNAFARDWLAERLGPIVTRVLTGIAGRTVEVRFVVWQPEVNPGEPAAPEIPASLQIGL
                     NPRYTFENFVVGKSNRLAQAAAQAVADNPAGPYNPLFLYGGVGLGKTHLLHAIAHACA
                     AAGQRPVYVSAEDFTNGLIDAIRTHSTAAFRDRYRQAEILLVDDVQFIAGKESTQEEF
                     FHTFNALHGQARQIVLSSDRSPKSFATLEERLRSRFEWGLAADIQPPDFETRVAILRS
                     KAERAGRTVQHEHLELIARRAQNNIRELEGALTRAFALADLTGAELGEETIEASLSEL
                     ASKTSRLTPGRIIAAVAQQFQVPEADLCGPRRTRQLALPRQVAMYLIREETDESLPRI
                     GQALGGRDHTTVMYGYERIADRLETDEGLRRQVIAIRSSLFQ"
     gene            complement(1919..>3128)
                     /gene="radA"
                     /locus_tag="VI520_04895"
     CDS             complement(1919..>3128)
                     /gene="radA"
                     /locus_tag="VI520_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLF37255.1"
                     /translation="RLDQIETGNDDRLPLGIAEFARVLGGGVVPGSLVLIGGDPGIGK
                     STLLLQASLGMAARGPVLYVSGEESVGQIKNRAARLTNGSAAADRIYLLTETDLGRVI
                     GHIEELRPLLTVIDSIQTMQSDDIDSAAGSISQVRECAARLQATAKSSGVAIFLVGHV
                     TKEGAIAGPRVLEHIVDTVLYLEGDSFHSYRLLRSVKNRFGPTNEVGVFEMGSSGLVE
                     VANPSEAFLAERKADAPGSAIAITMEGTRPLLVEVQGLTSPSAFGYPRRTSNGVDNNR
                     LLLLVAVLSRRVGIQLSDQDVFVNVVGGLQVSEPAADLALASAIASSARDRALPPDLA
                     LVGEVGLSGELRSVAQLPARLSEASKLGFREVVVPTTARGGVPAPAGLKVSPAASLAE
                     ALSLVLRGGG"
BASE COUNT          587 a         1032 c          958 g          551 t
ORIGIN      
        1 gccgacgcct ttgtggcgaa gcccttctca atcaatcagc tgcgggccgt catcaatacc
       61 gtgatggccc cgatccgggg ttaaagaaaa cgccgccagc ttggcggcgg gtggaatctc
      121 tccgctccat cccactcact ggaacagtga actccgaatg gcgatgacct gcctgcgcag
      181 cccctcgtcg gtctcaagcc ggtctgcaat tcgctcatag ccatacatca ccgtggtatg
      241 gtcacggccg cccagcgcct ggccaatccg ggggagcgat tcatcggttt cctcccggat
      301 cagatacatc gccacttgcc gcggcagggc gagctgccga gtccgccgcg gcccgcacag
      361 gtccgcctcc ggcacttgga actgctgtgc cacggcagcg atgatcctcc cgggggtcag
      421 ccggctggtc ttggaggcca actccgaaag ggaggcctcg atcgtctcct cccccagctc
      481 ggccccggtg agatctgcaa gggcgaaggc ccgggtcagt gccccttcca gctcccgaat
      541 gttgttttgg gctcggcggg caatcagctc cagatgctca tgctgtacgg tccggccggc
      601 ccgctcggcc ttggagcgca ggatcgcgac tcgggtctcg aaatcaggtg gttggatgtc
      661 ggctgccagt ccccactcga accgtgagcg cagccgttcc tccagggttg cgaaggactt
      721 tggcgaccga tcagaggaca agacgatctg cctggcctgt ccgtgcagcg cattgaaggt
      781 gtgaaagaac tcctcctggg ttgattcctt gcccgcaatg aactgaacat cgtcgaccag
      841 caggatctcc gcctggcggt agcgatcccg gaatgcggcc gtgctgtggg tacggatggc
      901 atcgatcagc ccgttggtga aatcctcggc ggagacgtag acgggccgct gcccggcggc
      961 ggcacaggca tgcgcgatcg catgcagcag gtgagtcttc ccaagaccga ctccgccgta
     1021 gaggaatagc gggttgtagg ggccggccgg attatcggcg accgcctggg cagcggcctg
     1081 ggccagccga ttgctcttcc caacgacaaa gttctcaaag gtgtagcggg gattgagacc
     1141 gatttggaga ctggccggga tctccggggc ggccggctcc ccggggttga cttcgggttg
     1201 ccagaccaca aaacggacct ccacggtcct tcccgcgatc ccggtcaaca ctcgagtgac
     1261 aatcggcccc agccgttcgg ccaaccaatc ccgggcgaat gcattctgca ccccgacaat
     1321 gaactggccg tcttcatagg ccagcagctc tgcgtcgcgc acccaggtat caaaggccgc
     1381 ttttggcatc tcgacctgca attggcccag cgcagcctgc cagagtcgct ccgcgttcat
     1441 gcggacctcc gagaatcgcc cgtgcgggcg aggggtgttg atcgtttgaa ttgtcgaaga
     1501 acgagctgtc gggctactgt tcctggagag gagggcccgt cgtctcgagc gcccgggccc
     1561 cccaagaccc gcgcgagacg aattctagca gagaggctcg aggcgatcaa tgctcgcgac
     1621 ccgcgccaat ctaaaaagat tcgaaacttt aagtgatggg cgccttaaag cagaacggca
     1681 gatctcagtg gcgaccgaac tcggtcagct ctgctcgacc gcccaacctg gccgggcttc
     1741 ggtaagctgc ccgccagatg tatgcccaag atcgatgctc cgaaccaggc cgaatcgagt
     1801 gcgcaccgaa tcttaagagc tggaggtcgg accggctgcc cgaactctag caggcgaaac
     1861 ccggaatccg cacttccagc gcgccgtcgt tgaggatgcg tagttcggaa gccatgaatc
     1921 agccgccgcc gcgcagaact agtgacaacg cctcggcgag gctcgccgcc gggctgacct
     1981 ttagccctgc aggggcgggc acgccgccgc gagcggtcgt gggaaccacc acttcccgaa
     2041 agccgagttt cgaggcctca ctcaatcggg ccggcaattg cgccacgctc cgcagctctc
     2101 cggacagacc cacctcccct accagggcga gatcgggggg caatgcccgg tcgcgagcgg
     2161 acgaggcgat ggcgctggcc agtgccagat ctgccgctgg ttcgctgact tgcagcccgc
     2221 ccaccacgtt gacaaacaca tcctggtcag agagctggat tcccacccgc cgactaagca
     2281 cggctacaag cagcagcagc cgattgttat ccaccccgtt ggaggtgcgc cggggatagc
     2341 cgaacgcgct cgggctggtg aggccctgaa cttccaccaa cagcgggcgg gtgccttcca
     2401 tagtgatcgc aatcgcagac cccggggcat cggcctttcg ctcagccagg aacgcctccg
     2461 aggggttggc gacttccacc aaacccgaac ttcccatttc gaaaactccc acctcgttcg
     2521 tcgggccaaa gcgattcttg acggagcgca gcaatcggta gctgtggaac gagtcgcctt
     2581 cgaggtaaag cacggtgtcg acgatgtgct cgagcacccg tgggccggcg atggcgcctt
     2641 ccttagttac atgtcccacc aggaagatcg ccactccgga agacttggcg gtggcctgca
     2701 gcctggccgc gcactcgcgg acctgggaga ttgatccggc tgccgaatcg atatcgtcgg
     2761 attgcatggt ctgaattgag tcgatgaccg tcagcagcgg acgcagctct tcgatgtggc
     2821 cgatcaccct cccaagatca gtctcggtga gcagatagat ccggtctgcc gcggccgagc
     2881 cattcgtcag gcgggcggcc cgattcttga tctgacccac cgattcctcg cccgagacat
     2941 acaagaccgg cccgcgggca gccatcccga ggcttgcctg cagcaatagg gttgatttgc
     3001 cgattccggg gtctcccccg atcagcacca gcgaccccgg gaccaccccg ccccccaaca
     3061 ctcgggcgaa ctcggcgata ccgagcggca gccggtcgtc atttcctgtc tcgatctggt
     3121 ccagccgc
//
LOCUS       DATDPQ010000214         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2445418, whole genome shotgun sequence.
ACCESSION   DATDPQ010000214 DATDPQ010000000
VERSION     DATDPQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445418"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1344)
                     /locus_tag="VI520_04900"
     CDS             complement(88..1344)
                     /locus_tag="VI520_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF37256.1"
                     /translation="MQRKSLIAGLAVLAVLLAACQTAAEPAAGEVEVFSWWTAGGEAD
                     GLAALIQVMDSENPDLQFVNSAVAGGAGTNARAVLATRLSADDPPDSWQGHAGQELIG
                     TYVAAGEIQPLNDLYESNGWLDVMPETLIPLISQDGNIYSVPVNIHRANVLWYNPEVL
                     ASNSVAVPSTIADFIAAAETLQAAGVTPLAMGEQWTSMHLFETVLLGTVGAETYNGLW
                     AGTADWAGPEVTQAIDNFSQILAYTNSDASTLSWDKAAQLVIDGDAAFTIMGDWAEGY
                     FKAAGLSPMEGFGWAPAPGTAGLFQFLSDSFVLPVGAPHPEAATAFLTVAGSKSGQEA
                     FNPIKGSICARTDCDPALFDEYLQSAMDDWASNTVVGSLTHGVVANDNWKSQIDTALG
                     LYLSDGDAAAFQAALVTACSDAGTCG"
     gene            complement(1591..>2146)
                     /locus_tag="VI520_04905"
     CDS             complement(1591..>2146)
                     /locus_tag="VI520_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37257.1"
                     /translation="DVLGDLVYPLTNRVWNSMCPAWSPEGSTIAFTTDDFFGDLQIHL
                     LDLSDGSKRQVTTGPDPAWDPSWSPDGSRIAFDSEGEGNYELYAVTMDGTRTERLTKD
                     SALDRRPDWSPDGQFIVFSSARSGSLELYVMKSDGTEVQRLEAGAGSNFDPAWSPDGL
                     QLAFVSNRDGNYEIYRIDLPALAP"
BASE COUNT          395 a          691 c          674 g          386 t
ORIGIN      
        1 tcactcggct cgaagagagt gggctgaaaa ggaaagtggg gcgggccgca gccggcccac
       61 cccactcgca ttccttacaa gcgaggccta cccgcacgtt cctgcgtcgg agcaggccgt
      121 gacgagcgcc gcctggaacg cggccgcgtc gccatccgag agatacagcc cgagagcggt
      181 atcaatctgc gacttccagt tgtcattggc caccaccccg tgggtcaggc tacccaccac
      241 ggtgttggaa gcccagtcgt ccatcgccga ctgcaggtac tcgtcgaata gtgctggatc
      301 acagtcggtc cgggcgcaga tcgaaccctt gatcgggttg aacgcctcct ggccggactt
      361 ggagccggcg accgtcagga aggctgtggc cgcctccggg tgcggagcgc caaccggcag
      421 cacgaagctg tccgacagga actggaagag cccggcggtg cccggagcgg gcgcccagcc
      481 gaagccctcc atcggtgaga ggccggcggc cttgaagtag ccctcggccc agtcgcccat
      541 gatcgtgaag gccgcgtcac cgtcgatcac gagctgagcg gccttgtccc aactgagggt
      601 agatgcgtcg ctgttggtgt acgccagaat ctggctgaag ttgtcgatgg cctgggtcac
      661 ttcgggcccg gcccagtcgg cagtcccggc ccacaatccg ttgtaggtct cggcgcctac
      721 cgtcccgagc agcacggtct cgaacaggtg catcgaggtc cactgctcgc ccattgccag
      781 cggcgtcact ccggccgcct gcagtgtttc ggcggcggcg atgaagtcag caatagtcga
      841 aggaaccgcc acgctgttgc tggccaggac ctccgggttg taccagagca cgttggcccg
      901 gtggatgttg accgggacgg agtagatgtt gccgtcctgc gagatgagcg ggatcagcgt
      961 ttcgggcatc acatccaacc agccgttgct ctcatacagg tcgttcagcg gctggatctc
     1021 gccggcagct acgtaggtgc cgatcaactc ctgcccggcg tggccctgcc acgagtcggg
     1081 aggatcatcg gcagacaggc gggtcgccag cactgctcgg gcgttggtcc ccgcaccccc
     1141 ggcaaccgcc gagttgacga actgaaggtc ggggttctct gagtccatca cctggatcag
     1201 cgccgccagg ccatcggcct cccctccggc ggtccaccag ctgaacactt ccacttcacc
     1261 ggcagctggc tcggccgcgg tctggcaagc tgccaagagc accgcaagca cggccaatcc
     1321 ggcaatcaag ctctttcgct gcatttgaac tttcctcctg agatgattgg cccggtaaac
     1381 gtcccccggg agattgacgt aggcgagggg tccggcctcg ggacgggcgt atcctcctta
     1441 cccgcaactg cgcgctttac cactctcgca gtcggttcga acgccgggaa tgtggcttca
     1501 gtataacgca gacggacaca acttcgggtc aggcttgagt ggcgggttga gcgggcccgc
     1561 cgcccggcct agtcgcgggg gtgggaagat ttacggggca agcgcaggga gatcgatgcg
     1621 gtagatctca tagttgccgt cgcggttcga cacgaaggca agctgcagcc catccggcga
     1681 ccaggccgga tcgaagttgc tccccgcccc ggcctccagc cgttgaacct cggtgccatc
     1741 gctcttcatg acgtagagct cgagtgaacc gcttcgggcg gagctgaaaa cgatgaattg
     1801 gccatccgga gaccagtccg gccttctgtc taaagccgag tctttcgtca acctctcggt
     1861 gcgcgtacca tccatcgtta ccgcataaag ctcataattc ccttcccctt ctgagtcaaa
     1921 tgcgatacgg ctcccatcgg gagaccagga tggatcccac gcagggtccg ggccggtcgt
     1981 cacctgccgc ttgctgccgt cgctgaggtc gagaagatgt atttgcagat cgccgaagaa
     2041 gtcatcagtc gtgaaggcga tcgtcgatcc ttcgggcgac caggccgggc acatactatt
     2101 ccaaacccga ttggtcaggg ggtaaaccag gtcccccaag acatcg
//
LOCUS       DATDPQ010000215         4293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1156158, whole genome shotgun sequence.
ACCESSION   DATDPQ010000215 DATDPQ010000000
VERSION     DATDPQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156158"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VI520_04910"
     CDS             <1..1098
                     /locus_tag="VI520_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HLF37258.1"
                     /translation="VFLSSPELWQRGHGPQHPLKPERLQRTFELLSEYGSFAAPNVQV
                     REPHLAEPEELALFHTQDYIRVTRALSAGELTLPAGSYGFGPGDNPIFSGMYETEALK
                     VGSALQSAELLYNGDCEVAFSYSGGLHHAGPDFASGFCVFNDCAVAIQWLLERGLRVA
                     YIDIDVHHGDGVQNAFYDSDRVLTISLHQDPRTLFPGTGFVEEIGRGAGTGYSVNVPL
                     PPLTFDAAYLRAFEAIVPPLLQRFSPDIVATQLGVDTHFTDPLANLALTTQAHLALFQ
                     ALDGLSPRWLAFGGGGYAIELVPRSWALAFGVMSGQQLPEELPEEYLSRYGGAWLHDQ
                     AGPEFSPTLRAQVEESVDDVIHHVRRTHRLE"
     gene            1371..2525
                     /locus_tag="VI520_04915"
     CDS             1371..2525
                     /locus_tag="VI520_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HLF37259.1"
                     /translation="MDPDRFRDSTAGKPILAPGGYWAFVPNPLPPALAWSTDLVAQLA
                     KAERSLAALAEKGSDFASPDTLVQPFIRREAVLSSRIEGTLTSYDELYRFETDPRSGG
                     EDALEVANYVRALEHGINRLETLPVSLRLIRETHAELLHGLDRQLATPGEFRRSQNWI
                     GPPGSTVESAPYVPPPVDAMLAGLDMLERYVHGRDDLPVLVRVGLIHYQFEAIHPFLD
                     GNGRIGRLLIALLLVLWDLMPNPWLFLSGYFDANRQAYYDGLLGVSERGDWEAWLLLF
                     LEAVARQSDEAAAALDRLEALRGHYEAMLRKERAAGRLMQVVDFLLGHPMATVRQIER
                     GIEARDFKVAQRYVEKLVERGVLREFTGRKRNRIYRADQIFEAIEGTLDV"
     gene            complement(2551..2623)
                     /locus_tag="VI520_04920"
     tRNA            complement(2551..2623)
                     /locus_tag="VI520_04920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2588..2590),aa:Arg,seq:cct)
     gene            complement(2686..3759)
                     /locus_tag="VI520_04925"
     CDS             complement(2686..3759)
                     /locus_tag="VI520_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF37260.1"
                     /translation="MDLSSAFGRQPRILVADDDWLNRDLLQAYLTTSGCEVVTAADGE
                     TALRLALNLPPDLALIDVQMPRLDGLKLCHKLKNTPATRFVPVVIITALDSEEEKLNA
                     FEAGADDFITKPYSSIVLLARVRSLLRIKRLHDEVESRNALLRQVLDRYVAEDVADVI
                     LTDPERHLKLFGETRPVTVLFADIRGFVRYTELHTGPEVIRTLNHIFQAVSKVVFAHK
                     GTFDKYLGDGLMAFYGAPVAGEDDAQRAVATAVEMQRLFAKLRENPDVDLKGLGLGIG
                     LHSGEAIVGNIGSEQVMDYTVVGDVVNVAKRLQDVAQSGQILFSQDTFSELKGVEAKK
                     LKPLKLPGREERVTPYLIELAKR"
     gene            complement(3812..>4293)
                     /locus_tag="VI520_04930"
     CDS             complement(3812..>4293)
                     /locus_tag="VI520_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLF37261.1"
                     /translation="KVLVRDVGSSITVEPLLGLPVVKDMIVDMEPFWAHYRSVKPFFI
                     NEDPSPERERLQSPEQRARFDDSTKCILCAACTTSCPSFWADGEYVGPAAIVQAHRFI
                     FDSRDAAAGQRLEILGEQMGVWRCRTIFNCTVACPRDIKVTQAIGEVKQAIATGQLD"
BASE COUNT          765 a         1385 c         1314 g          829 t
ORIGIN      
        1 gtatttctgt cttcgccgga gctctggcag cgggggcatg ggccgcagca tccgctgaag
       61 cccgagcgcc tgcagcgcac cttcgagctg ctcagcgaat acggctcatt cgccgcgccc
      121 aatgtgcagg tcagggagcc gcacctggcc gagccggaag agctggcgct gttccacacc
      181 caggactaca tccgggtgac tcgggcactc agcgcaggtg agttgacctt gccggccggc
      241 agctacggtt tcggcccggg cgacaacccg atcttctccg gtatgtacga aaccgaagcg
      301 ttgaaggttg ggagcgccct gcagagcgcc gaactgctct acaacgggga ttgcgaggtg
      361 gccttcagct acagcggcgg gctgcaccac gccggcccgg acttcgcctc cggcttctgc
      421 gtattcaacg actgcgcggt cgcaatccag tggctgctgg agcgggggtt gcgggtggct
      481 tacatcgaca ttgacgttca ccacggcgac ggagtccaga acgccttcta cgacagcgac
      541 cgggtgctca ccatctcgct acatcaggac ccgcgcaccc tgtttcccgg caccggtttc
      601 gtcgaggaga tcggccgggg cgccgggacc gggtacagtg tcaatgtccc cctgccgccg
      661 ctgaccttcg acgcagccta cctgcgggcg ttcgaggcca tcgtgccgcc gctgctgcag
      721 cgcttctcgc ccgacatcgt ggcaactcaa ttgggtgtag atacccattt cacggacccg
      781 cttgccaacc tggccctcac gacccaggcc catctggccc tattccaggc actggacggt
      841 ttatcgccgc gctggctggc ctttggcggt ggtggctacg ccatcgagct ggtcccgcgc
      901 tcctgggcgt tggcatttgg ggtgatgtca gggcagcaat tgccggaaga gctgcctgaa
      961 gaatacctga gccgctacgg tggcgcctgg ctgcacgatc aggccgggcc cgagttcagc
     1021 ccaacccttc gggcgcaggt ggaggagtca gtcgacgacg tgatccacca cgtcagacgg
     1081 acccacaggc tggagtaaac ccacgatcgc cgactcctta caagcgtcga tgaccgagcg
     1141 acccctgaac gcgccggcgt tggcggctcc tgaaccctcg gcgtcagcgg ctgctgagct
     1201 ccccgcgtcg gcggctcctg aacgcctctt ctccggcctc ctcgactgtc aatacaagag
     1261 cttcttattg acactttagc catcttgtgg gaatattggg ctatcctatt ggtaaataag
     1321 gctcaaagtg ccaataatgg aggtccttat tggcagtttg gggatgagag atggatccag
     1381 accgatttcg cgacagcacc gccggaaagc cgatcctggc acccggcggg tattgggcct
     1441 tcgtccccaa cccactcccc ccggcgctgg cctggtcgac cgacctggtc gcccagctgg
     1501 ctaaggccga gcgcagcctg gccgccctgg cagagaaggg tagcgatttc gcaagtcccg
     1561 acacactggt ccaaccattc atccgtagag aggcagtcct ctcctcgcga atcgagggca
     1621 ccctgacatc gtacgacgaa ctctatcggt tcgaaaccga tccgcgcagc ggtggtgagg
     1681 acgctctgga ggtcgccaac tacgtgcgcg cacttgagca cggcatcaac cggcttgaga
     1741 ccctgcctgt aagcttgcga ttgattcgag aaacccacgc cgagctgttg cacggcctgg
     1801 atcgacagct tgcgactccc ggcgagttcc gccggtctca gaactggatc ggcccacccg
     1861 gcagcacggt cgagagcgcg ccgtatgtgc cgcctccggt ggatgccatg ctggcgggat
     1921 tggacatgct ggagcgctac gttcacggtc gggacgatct tccagtgctg gtccgagtcg
     1981 gcctgatcca ctatcaattc gaggcgatcc atccgttcct tgacggcaat ggccggatcg
     2041 gccggctgct cattgccctg ctcctggtcc tctgggatct gatgcccaat ccctggttgt
     2101 tcctgagcgg ttatttcgac gccaaccgtc aggcttacta cgacgggctg ctgggcgtta
     2161 gcgaacgggg tgattgggaa gcgtggctgc ttctattcct ggaagcggtt gcgcgccagt
     2221 cagacgaggc cgcggcggcc ctcgaccgat tggaagcgct ccgggggcat tatgaggcga
     2281 tgctgcgcaa agagcgcgct gcgggcaggc tcatgcaggt ggtcgatttc ttgcttggcc
     2341 atccgatggc gaccgttcga cagatcgagc ggggaatcga agcgagagac ttcaaggtcg
     2401 cccagcgtta cgtcgagaag ctcgtcgagc gcggggtgct gcgcgagttc accggtcgaa
     2461 agcgcaaccg gatctaccgc gctgatcaga tcttcgaggc gatcgagggc acgctcgatg
     2521 tctaagagcc gaccgcaccg aaattaggta tgccccaggc gcgaatcgaa cgcgcaacct
     2581 tggccttagg agtgccctgc tctatccttt gagctactgg ggcccgttcg gattatatct
     2641 ccccgcctga tcctcgcggg caacctgcga cccaggctcc acgcttcagc gcttcgctag
     2701 ttcaatcaaa taaggcgtga cccgctcctc gcggccaggc agcttgagcg gtttcagctt
     2761 cttggcctcc acccccttca attcactaaa agtgtcctgg ctgaacagga tctgcccgct
     2821 ttgggcgacg tcctgcaagc gcttagccac gttgaccaca tccccgacga cggtgtagtc
     2881 catcacttgc tcggagccaa tgttgccgac gatcgcctcg cccgagtgca ggccaattcc
     2941 gagtccgagg cccttcaaat cgacatctgg attctcccgc aacttggcga agagccgctg
     3001 catctcgacc gccgtggcca ccgcccgctg ggcgtcgtct tcgcccgcca ccggggcgcc
     3061 gtagaacgcc atcaggccat cgcccaggta tttgtcgaag gtacccttgt gagcgaagac
     3121 tactttgctg actgcctgga agatgtgatt caaggtccgg atgacctccg ggccggtgtg
     3181 cagctcggtg taacgaacga atccccggat gtcggcaaac agcacggtca ccggccgggt
     3241 ctcgccaaac agcttgaggt gccgttccgg gtcagtcagg atgacatccg ccacgtcttc
     3301 cgcgacgtag cggtccagta cctgtctgag cagcgcgttg cgcgattcca cctcgtcgtg
     3361 caatcgtttg atgcggagca gcgagcgcac ccgggccagc agcacgatcg agctgtacgg
     3421 tttggtaatg aagtcgtcag ctccggcctc gaaggcattg agtttctctt cttcactgtc
     3481 gagggcggtg atgatgacca ccggcacgaa gcgggtcgcg ggcgtgttct tgagtttgtg
     3541 gcagagtttc agtccatcca gccgcggcat ctggacgtcg atcagcgcca gatcgggggg
     3601 cagattcaaa gccagccgca gcgcggtctc gccgtcggcc gcggtgacca cctcacagcc
     3661 ggaggtcgtc aggtaggcct gcagcaggtc ccggttcagc caatcgtcgt ccgccaccag
     3721 gatccgcggc tggcgcccaa aggccgacga caggtccatc gctgcatctt gcagcgtccg
     3781 cgcattcctc atcgttcgcg ccgaccggat ctcaatctag ctgcccggtg gcgatagcct
     3841 gcttcacctc gccaatcgct tgagtgactt tgatgtcccg tgggcaggcc accgtgcagt
     3901 tgaagattgt tcggcagcgc cagaccccca tctgctcgcc cagaatctcc agccgctgcc
     3961 cggccgccgc gtcccggctg tcaaaaatga aacggtgggc ctggacgatc gcggccggtc
     4021 ccacatactc gccgtccgcc caaaaggagg ggcaggaggt ggtgcaggcc gcacacagga
     4081 tgcacttggt ggagtcgtcg aaacgggccc gctgctccgg gctctgcagg cgctcccgct
     4141 cgggagatgg gtcctcgttg atgaagaacg gcttgaccga tcggtaatgg gcccagaacg
     4201 gttccatgtc cacaatcatg tctttcacga ccggcagccc gagcaaaggt tccacagtga
     4261 tcgagcttcc cacgtcccgc accaatactt tgc
//
LOCUS       DATDPQ010000216         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1926080, whole genome shotgun sequence.
ACCESSION   DATDPQ010000216 DATDPQ010000000
VERSION     DATDPQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926080"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VI520_04935"
     CDS             <1..1201
                     /locus_tag="VI520_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF37262.1"
                     /translation="EGHGEVQEMIDVCDFAVGLSRQLYGLTMQSERPAHRMYEQWHPL
                     GPIGVITAFNFPVAVWSWNAAIAAVCGDPVIWKPSSLVPLTAIAVQHLANQVMADHGL
                     SGVFSLVIGTGSEVGERLLTDPRLPLISFTGSTDIGRRVSEAVGRRFGRTILELGGNN
                     AIIVAEDANLDLATRAILFGAVGTAGQRCTSTRRIIVQQGVAEQLTERLVKGYHQVRI
                     GDPLKETTLMGPLVSPTAVEEMFTALDRIQQQGGTIVVGGKRRPELGPNFVEPTIVRM
                     PAQTDIVKEETFAPILYLLQFETIDEAIELHNDVPQGLSSAIFTDNMRTMETFLSARG
                     SDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWSKELPLA
                     QGIEFGS"
     gene            complement(1244..1801)
                     /locus_tag="VI520_04940"
     CDS             complement(1244..1801)
                     /locus_tag="VI520_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLF37263.1"
                     /translation="MRTLPLTALLLVLLAGCNFRGTESVTPTPSGDQVVQTAQAIAQA
                     TLAAATSTPTQVPPTPSPAPPTNTPPPAATLTPASPVVTANYNANIRRGPGENYEVLD
                     FLLGGQQADVFGRYDNSPIGTWWYVRPIGSGITGWVWSGAMALSGSDFGVPVLTPVPT
                     SAPTPESTSAPTQTPTATPTPTSTP"
     gene            1882..2817
                     /locus_tag="VI520_04945"
     CDS             1882..2817
                     /locus_tag="VI520_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37264.1"
                     /translation="MPRGQSELEADIAQTEQEAADPAIQRGREQLAIARERMLSAALA
                     FCDGTISAGQLRAVREFLREKDSHLAKLEGATPPVFVEDPPAQAEVGLIGEETLTDRS
                     IKETLRLELETEDAPELMEMLTALDEKLDRLEDDFEHGRINASQYRAIRRHYQEQREV
                     ALRLKQAHPRSDRWRVVLEEGRTTFLMQLNEAQCTCVALFDLNSRDRIFLQGDMPPAA
                     EEAMGLLRTFGPPRKESGPGRMLATHLDDASCLLLIPGHFTAVLAIFSQDPPGWQVRA
                     LKEVLLNFEAANRTGLRKSQHEKLVFPDLRRFVKS"
BASE COUNT          548 a          902 c          937 g          497 t
ORIGIN      
        1 cgaaggccac ggcgaagttc aggagatgat cgacgtctgt gatttcgcgg tcggcctatc
       61 ccgccagctc tacggattga caatgcagtc tgaacggccg gcccaccgca tgtacgaaca
      121 gtggcatccg ctgggcccga ttggggtcat caccgccttc aatttcccgg tcgcggtctg
      181 gtcgtggaac gcggccattg ctgcggtctg cggggatccg gtcatctgga aaccttccag
      241 cctggtcccg ctcaccgcga tcgcggtcca gcatctggcc aatcaggtga tggccgatca
      301 cggcctatcc ggagttttca gtttggtgat tgggaccggt tccgaggttg gggagcgact
      361 gctgaccgat ccgcgcctcc cgctgatttc ttttaccgga tcgaccgaca tcgggcgccg
      421 agtctcggaa gccgtcgggc ggcgcttcgg tcggacgatc ctggagctcg gcggcaacaa
      481 cgctatcatc gtggcggagg acgccaatct ggacctggcc acccgggcca tcctgtttgg
      541 agcggtgggc accgccggtc agcgctgcac ctcgacccgc cggatcatcg ttcagcaggg
      601 agttgccgag cagctcaccg agcgattggt caagggttac caccaggtgc ggattggcga
      661 cccgctgaag gaaaccaccc tcatgggccc gctggtcagt ccgacggcgg tcgaggaaat
      721 gttcacggcc ctcgaccgga tccagcagca gggaggcacg atcgtggtgg gcggcaaacg
      781 ccggccggag ctgggcccga acttcgtcga acccaccatc gtgcggatgc cggcccagac
      841 cgacattgta aaggaagaga ccttcgcccc gatcctgtat ctgctgcagt tcgagacaat
      901 cgatgaagca atcgaactgc acaacgatgt gcctcagggg ctgtcgagcg caatcttcac
      961 cgacaacatg cgcacgatgg agacgttcct ctcggctcgc ggctccgact gcgggattgc
     1021 caacgtcaac atcggaacct ccggggccga gatcggcggg gctttcgggg gtgagaaaga
     1081 aaccgggggc ggccgagagt ccggctccga cgcctggaag gcctacatgc ggcgccagac
     1141 caataccatc aattggtcga aggaattgcc gctggcgcag gggatcgagt tcggaagtta
     1201 ggccggcagg ctagaccgca ggtacagcgg cggcacaggc gggctaagga gtgctcgtcg
     1261 gagtcggtgt cgccgtcgga gtctgggtgg gcgcgctggt cgactcgggt gtcggggctg
     1321 aggtcggcac cggagtcagc actgggaccc caaaatcgct cccgctcagc gccatggccc
     1381 ccgaccacac ccagccggta atcccactcc caatcggccg cacgtaccac caggtaccga
     1441 tcggcgaatt gtcgtaccgt ccaaacacat ccgcctgctg tccgccaagc agaaaatcaa
     1501 ggacttcata gttttcccct ggcccacgtc ggatattggc gttgtagttg gcagttacta
     1561 cgggcgacgc cggcgtgagg gtagctgccg gcgggggagt attcgtcggc ggggctgggg
     1621 aaggtgtggg cggcacctgg gtcggggtgc tggtcgccgc agccagagta gcctgggcga
     1681 tcgcctgggc ggtctgcacg acttgatccc cagaaggggt tggggtgacc gactccgtgc
     1741 ctcggaaatt gcacccggcc aagaggacta acaagagcgc cgtaagcggc aaagtgcgca
     1801 ttagaacttg agtatatcgc ggtcggctgc tggtacccca gtcatctgaa cgccgttcac
     1861 ccgagcctgt atgatagggc catgcccagg ggccagtccg aactcgaagc cgatatcgcc
     1921 cagaccgagc aggaggccgc cgatccggcc atccagcgcg ggcgggaaca gctggcgatc
     1981 gcccgggaac ggatgctgtc ggccgccctg gcgttctgcg acggaaccat cagcgccggg
     2041 cagctgcgtg cggtgcggga gttcctccgg gagaaagata gccatttagc caagctggaa
     2101 ggagccaccc ccccggtatt tgtcgaagac ccaccggccc aggctgaggt tgggctgatc
     2161 ggcgaagaga ctctcacgga ccggtcgatc aaggagacct tgaggctgga actcgagacc
     2221 gaggacgctc ccgagctgat ggagatgctt accgccctcg atgaaaagct cgaccgctta
     2281 gaggacgatt tcgaacacgg ccggatcaac gcctcccagt accgggccat tcgccggcac
     2341 taccaggagc agcgggaggt cgccttgcgg ctcaagcagg ctcacccgcg cagcgaccgc
     2401 tggcgggtag tcctggaaga gggccgcacg actttcctca tgcagctcaa tgaggcgcag
     2461 tgtacctgtg tcgcgctgtt cgatctcaat tcgcgggacc gaatcttcct ccagggcgat
     2521 atgcctccgg cggccgaaga ggcgatgggc ctcctgcgga ccttcggccc gccccgcaaa
     2581 gaatccggcc ccggccggat gctggccacc cacctggacg acgcttcctg cctgctgttg
     2641 atccccggcc actttaccgc ggtcctggcc atcttctccc aagatccccc cggctggcag
     2701 gtccgggcgc ttaaggaagt gctgctgaac ttcgaagccg ccaatcggac ggggctccgc
     2761 aagtcccagc atgagaagct agtcttcccg gacttgcgcc ggtttgtgaa atcctgaaca
     2821 aatacttcca attgcgcccc aattcaaacg aaagggctgg tcagcccgtg aagagcagca
     2881 tggc
//
LOCUS       DATDPQ010000217         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_612868, whole genome shotgun sequence.
ACCESSION   DATDPQ010000217 DATDPQ010000000
VERSION     DATDPQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612868"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VI520_04950"
     CDS             <1..679
                     /locus_tag="VI520_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HLF37265.1"
                     /translation="LEALGCRVLLAVGQGCCGRTMISKGLLARARERANLNVRALGKY
                     AKQRVPIVGLEPSCLLMLRDEYLDLLPGDEDAIAIAATARLIEEFLTEATTAGSSRLA
                     GLDLSPRPGKVLLHNHCHSKALVGSGPVLAMLQAMRSEVEEIQSGCCGMAGSFGYEVE
                     HYALSMQIGELKLFPRIREEASRTPALRIVAPGMSCRSQIRDGTGATASHPVMMLAEA
                     LADQVPG"
     gene            complement(698..1696)
                     /locus_tag="VI520_04955"
     CDS             complement(698..1696)
                     /locus_tag="VI520_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLF37266.1"
                     /translation="MTALTLEGSFLDYLSAVRLARSPNTVRTYRNAVQAFQACLDSLG
                     VDSKTQPPRIANENWVGDFAAHLRNYAPASERLYLTAISGWYEFLSAEGLAELNLPRL
                     RSLIRRRARRPGQRLPQFPREAIEAILAAASDISNQGDDEPGRLRGLRDRALLFTLAD
                     TGLRVHEAVGLRRGDLDWNEGRALMIGKGDREAVVRFSNRSLRYLRAYLEARSSRDGS
                     SGRPLSALPLFARHDPASGKRVLPISTTTARAIVHERARQALGERPQAAITPHSFRHY
                     FVTTVLRGSGGNLKLAQELARHRSIAVTQRYAHLSDDELDRGYYDIFESGQAVAKD"
     gene            complement(2013..>2652)
                     /locus_tag="VI520_04960"
     CDS             complement(2013..>2652)
                     /locus_tag="VI520_04960"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HLF37267.1"
                     /translation="RARLQRSDELLLLQLKAVLRAMANAIEARAGVYTLRHVEAVNAY
                     AQAMALELGWDEFQLEALEFGAILHDVGKINIPEKILSKEGPLDESEWAEMRMHPVYG
                     AHMIEGNPYLDSALPLVLHHHERWDGNGYPDGLAGEAIPHGARLLAVADTFHAMTSDR
                     PYRSALAFEKAFAEILDQSGKQFDPQMVAAFERCWHRGDLARIHKSVLPDPE"
BASE COUNT          496 a          774 c          882 g          500 t
ORIGIN      
        1 gctggaagcg ctaggttgcc gagtgctgct cgcggtcggc caggggtgtt gtgggcgaac
       61 catgatctcg aagggtctgc tggcgcgggc gagagagcgg gccaatctga acgttcgggc
      121 cctggggaaa tacgccaaac agcgcgttcc gatagttggg ctcgagcctt cgtgcttgct
      181 catgctgcgg gacgagtacc tggacctcct gcccggtgat gaggatgcaa tcgcgattgc
      241 agccactgca agattgattg aggagttcct gaccgaggcc accacggcgg gaagcagtcg
      301 cctggcgggt ctcgaccttt ctcctcgccc cggcaaagtc ctgttgcata accactgtca
      361 ctccaaggca ctggtgggga gcgggccggt gctcgcaatg ctgcaggcga tgcggtccga
      421 ggtcgaagag atccagtcgg ggtgctgcgg gatggcaggc tcgttcgggt acgaggtgga
      481 gcactacgcg ctgtccatgc agatcgggga actaaagctc ttcccccgga tccgtgaaga
      541 ggcctcccgg actcctgcgc ttcggatcgt cgcccccggg atgtcttgtc gatcgcagat
      601 tcgagatgga accggggcta cggcctccca tccggtgatg atgttagccg aggcgctggc
      661 agatcaagtc ccgggatgag tggtcgagga gtcgagctca atccttggca accgcttggc
      721 cggattcgaa gatatcgtag tacccccgat cgagctcgtc gtccgagagg tgcgcatagc
      781 gttgggtaac cgcgatgctg cgatggcggg cgagctcctg ggccagcttc aggtttccgc
      841 cggaacccct gagcacggtc gtgacgaaat agtgccggaa agagtggggc gtgatcgcag
      901 cttggggtcg ctccccgagg gcctggcggg cgcgctcgtg gacaatcgcc cgggcggtgg
      961 tggtggagat cggcagcact cgtttgccgg aggctgggtc gtgtcgggcg aagagcggca
     1021 gtgcgctcag cggacggccg gaggagccgt cacgactcga ccgagcctca agataggcgc
     1081 gcaagtagcg cagcgaccgg tttgagaagc gaaccaccgc ttctcggtcg cctttgccga
     1141 tcatcagcgc ccggccctcg ttccagtcca ggtccccgcg gcgcagaccg actgcctcgt
     1201 gtacccgcag accagtgtct gccaaggtga acaagagggc ccgatcccgc aagccgcgca
     1261 gccggccggg ctcatcatct ccctggttgg agatgtccga ggcggcggcg aggattgcct
     1321 cgatggcttc ccgtgggaac tgaggcaagc gctggcccgg tctgcgcgct cgccggcgga
     1381 ttagggagcg cagccgaggc aaattgagct cagccagccc ctcagcggag aggaactcgt
     1441 accacccgga gatggcggtc agatacaggc gttcgctggc cggagcgtag tttcgcaggt
     1501 gggccgcgaa gtctccgacc caattctcgt tcgcgatccg tggaggttga gtcttcgaat
     1561 cgaccccaag gctatccagg caggcctgga aagcctgaac cgcattccga taagttctta
     1621 ccgtgttcgg gcttcgggcc aggcggacgg cactgaggta atccagaaat gatccctcga
     1681 gggtgagagc cgtcatgcgg ggaggactgt acaccagagt ccgtagcggg gcgatcgaac
     1741 tgagactgca atggtcacaa gcaggtcatt caacccgggt cttttgctgc tggagccgcg
     1801 cacgaattgc ttgcgataat gatacttatc ataaccatca tgagtgtatc ccgaccggac
     1861 ccatccgtca agtacctccc ccgccaactg gtgtcggcga tcggggcgat cggagctcag
     1921 tacagtaggc ctggccatga ccgaaggaac attgggcaag ctgggacggt gtccggttta
     1981 ccccccgccc cgtttgaggc ggagaagcgg agttactcgg ggtcgggcag aacgctcttg
     2041 tggatccggg ccaagtcgcc acggtgccag cagcgctcaa aggccgcgac catttgggga
     2101 tcgaactgct tgccggattg gtccaagatt tcggcgaagg ccttctcgaa ggcgagggcg
     2161 gaacggtagg gccggtcaga ggtcatagca tggaaagtat cggccactgc caagaggcgt
     2221 gccccatgcg gaatggcttc gccggccagg ccatccggat acccattccc gtcccaacgc
     2281 tcgtgatgat ggaggactag cggcagggcc gaatcgagat aggggttccc ttcaatcata
     2341 tgagcgccat agactggatg catgcgcatc tcggcccatt cactctcatc gagtggaccc
     2401 tctttcgaga ggattttttc ggggatgttt atcttgccga catcatgcaa gatagccccg
     2461 aactcgagcg cctcgagctg gaactcatcc caccccaatt cgagggccat tgcctgggcg
     2521 tacgcgttca ccgcctcgac atgccgcagg gtatacacgc cggcccgtgc ttcgatggcg
     2581 tttgccattg ctcgcaggac agctttgagt tgtaagagca gcagctcgtc gcttcgttga
     2641 agtcttgccc ga
//
LOCUS       DATDPQ010000218         3401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1413146, whole genome shotgun sequence.
ACCESSION   DATDPQ010000218 DATDPQ010000000
VERSION     DATDPQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413146"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VI520_04965"
     CDS             <1..324
                     /locus_tag="VI520_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLF37268.1"
                     /translation="LRKIEADQGRVRTADKYAPRTIDLDLSLFGDQVLSGSDYQIPDP
                     ELTRLAHLAIPLAELAPEFRHPSLDETLRQIADRLRPGSKLVRRPDLSEQLRGLLAPR
                     RSRGR"
     gene            314..1687
                     /locus_tag="VI520_04970"
     CDS             314..1687
                     /locus_tag="VI520_04970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLF37269.1"
                     /translation="MAAEVVICGAGIAGISTAYHLTVGRGLSDVVLVDERPPLSLTSD
                     KSTECYRNWWPGPGDAMVGLMNRSIDILEALARAHTNPFNLTRRGYLFASADPARIEE
                     FRTQGQEAASLGAGPLREHRGAPDDPTYVPAPASGFEGQPAGADLITDAPLIQRHFPY
                     LSETTVAVLHARRAGWFSAQQLGMLMLESARAAGLRFVTGKLDRVEQRKGRVQAVQIQ
                     TAEGPTRVALRHLVNAAGPYLKQVAAMVGVELPVFSELHAKVAFKDRLGAFPRSAPLL
                     IWTDPQRLPWKEDERQLLERDPSSRHLLDEFPRGVHGRPDGPADSPIVLLLWTYQTDP
                     VEPRFPLQFDPQHAEIGLRGMATMVPKLRAYFDAPPKPFIDGGYYTKTRENRPLVGPL
                     PVEGGWVIGALSGYGLMASPGCGELLAAHITGDELPSYARWFQLDRYRDPDYQEMLSN
                     WGRSGQL"
     gene            complement(1717..2604)
                     /locus_tag="VI520_04975"
     CDS             complement(1717..2604)
                     /locus_tag="VI520_04975"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLF37270.1"
                     /translation="MISLNGIFPPIPTPFDSDESIDFDKLRSNLTRWNTWPLAGFVVS
                     GSTGESVSLSGDERVELVRAVRSAIPSEKLLIAGAGLESTRETVRLVHRMAEAGADAV
                     IVVTPSYFAPSIGPAGLLAHYRSIAEASDLPVILYSVPIYTHLDLPLEVVRELATHPK
                     VIGIKESGGSLAKITSLVSQTPEDFQVLVGSAGLLLGALTVGAVGCVPALGNVAGNSI
                     HELLAAFQRGSWEQAREIQTRLVEPNELLSSRFGVPGVKAALDLLGFYGGPLRSPLRP
                     LGEPELAVLRDILVRAGLL"
     gene            complement(2636..3268)
                     /locus_tag="VI520_04980"
     CDS             complement(2636..3268)
                     /locus_tag="VI520_04980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLF37271.1"
                     /translation="MRIYDISVGISPEIPVWPGDAPVRLERVQSMDAGAHANVSRFEA
                     GVHVGTHIDAPLHFIPGGRSVEAIPLKSLIGRAYVIELKRAEVLDASTLEGAGIPPRT
                     RRLLFKTRNSEYWTKGEPKFRRDFVAVDSSGAEWLVRKGVRLVGVDYLSVAPFGDAAP
                     THRILLEAGVVIVEGLNLAAVARGRYTIYCLPLKLIGSDGAPARVILVGV"
BASE COUNT          632 a         1105 c         1079 g          585 t
ORIGIN      
        1 ctgcgcaaga ttgaggccga tcagggccgg gtgcggacgg ccgacaagta tgcgccgcga
       61 accattgacc tcgatctgtc cctgttcggc gatcaggtct tgagcggctc cgattaccag
      121 atcccggatc cggagctcac ccggctggcc catctcgcaa ttcccctggc agaattggcc
      181 cccgagtttc gccacccaag cctcgacgag accctgcgcc agatcgcgga ccggctacgt
      241 cccggatcga agcttgtccg ccgtccagac ctaagcgagc agttgcgcgg gctgctggct
      301 ccgcgtcgct cccgtggccg ctgaagtcgt tatctgcgga gcggggatcg ccgggatttc
      361 taccgcctat catctgacgg tcggccgcgg cctctccgac gtcgtactgg tcgatgagcg
      421 ccctcccctc tcgctgacca gcgataagtc caccgagtgt tatcggaatt ggtggcccgg
      481 gccgggagac gcgatggtgg ggttgatgaa ccgcagcatc gatatcctcg aggcgctggc
      541 ccgcgcccac acgaatccgt tcaacctgac ccgccgcggt tatctcttcg cctcggccga
      601 cccggcccgg atcgaagaat tccgaacaca gggccaggag gccgcttcgc tcggcgccgg
      661 cccactgcgt gagcaccgcg gcgcgccgga cgatccgacg tatgtcccgg cgccggcatc
      721 cgggttcgaa ggccagccgg ctggggccga cctgatcacc gacgccccac tgatccaacg
      781 gcacttccct tatctcagcg aaacgaccgt tgcggtcctg catgctcggc gggcgggttg
      841 gtttagcgcc cagcagctcg gcatgttgat gctcgaaagt gctcgggcgg ctggccttcg
      901 attcgtgaca ggcaagctgg accgggtgga gcagcggaaa ggccgggtgc aggcggtgca
      961 gattcagact gcagagggac caacccgggt ggcgcttcgg catttggtca acgccgccgg
     1021 cccgtacctc aaacaggttg cggccatggt gggagtcgag cttccagtgt tctccgaact
     1081 gcatgccaaa gtggcgttca aggaccgact gggcgcgttt ccgcggtccg caccgctgct
     1141 gatttggacc gatccgcagc gccttccgtg gaaggaagac gaacgtcaac tgctcgaaag
     1201 agatccgtcg agccgccacc tgctggatga attcccgcgg ggcgtgcacg gccggccgga
     1261 tggaccggcc gacagcccga tcgtcttgct gctctggacc tatcagacgg acccggtgga
     1321 gccgcgcttc ccgctgcagt tcgacccgca gcatgccgag atcggcttgc gcgggatggc
     1381 gacgatggtc ccgaagctgc gggcctattt cgatgcgccg cccaaaccgt tcattgacgg
     1441 cggctactat acgaagactc gcgagaatcg gcccctggtc ggccccctgc cagtcgaagg
     1501 aggctgggtg attggggcgc tatccggcta tggcctcatg gcctcgccgg gctgcggcga
     1561 actgctggcc gcccacatca ccggcgacga gctgccgagc tatgcccgct ggtttcagtt
     1621 ggatcgttac cgcgatccgg actaccaaga gatgctctcg aactggggcc gcagcggtca
     1681 gctgtgaaat cccggcgcgc cgaaaggaag gcccggctag agcaggccgg cccgcacgag
     1741 gatgtcgcgc agaacagcca gttcgggttc gccgagcggc cgcagtggtg agcggagcgg
     1801 tccgccgtag aagcccagca ggtcgagggc ggctttgacc ccgggtactc cgaagcgcga
     1861 gctgaggagt tcgttcggtt cgaccagccg ggtctgaatc tccctggcct gctcccaact
     1921 gccgcgttgg aacgcagcca gcaattcatg gatcgagttt cccgcgacat ttccgagagc
     1981 aggaacgcat ccgaccgccc caactgtcag cgccccgagc aacagcccgg cggatccaac
     2041 cagcacttgg aaatcttctg gggtctgaga caccagcgac gtaatcttgg ccaggctgcc
     2101 gccgctctcc ttgattccga tcaccttcgg gtgggtggcg agttcccgga ccacctcgag
     2161 cggcaggtcg aggtgggtat agatcggcac gctgtagagg atcaccggga ggtccgaggc
     2221 ctccgcgatc gagcggtagt gggccagcaa cccagcaggc ccgatgctgg gggcgaaata
     2281 gctcggggtg acgacgatca ccgcatccgc tccggcttcc gccatccgat ggaccaacct
     2341 gaccgtctcg cgagttgact ccaggcctgc accggcgatc agcagctttt cgctcgggat
     2401 ggccgaccga accgctcgaa ccagttcaac tcgctcatca ccactgagtg agaccgactc
     2461 tccggtcgag ccggacacca caaagccggc aagcggccaa gtgttccaac gggtcagatt
     2521 ggatcttagc ttgtcgaaat cgatcgattc gtcagagtcg aagggggtcg ggatcggagg
     2581 gaagatgccg ttcagcgaaa tcaagggtcg ggcggggctt cgccggttgg acagttcaga
     2641 cgccgaccag gatcacccgg gccggcgcgc catcacttcc gataagcttg agcggcaggc
     2701 agtagatcgt atagcgacct cgagccaccg cggccaggtt caatccctcg acaatcacta
     2761 ccccggcctc cagcaagatg cgatgggtcg gcgctgcatc tccgaatggt gcgaccgata
     2821 gatagtcaac accgaccaac cggaccccct tgcgaaccaa ccattcggcc ccgctggaat
     2881 caacggcgac aaagtcacgc cgaaacttgg gctcgccctt ggtccaatat tcggagttgc
     2941 gagtcttgaa caacagccgc cgggtacgag gcgggatccc ggcgccttct agagtggagg
     3001 cgtccaagac ttccgctcgc ttcaactcaa tgacgtaggc ccgcccgatc agtgacttga
     3061 gtggaattgc ttccacactg cgtccgccgg ggatgaagtg cagtggagca tcgatgtggg
     3121 tgcctacgtg cacaccggcc tcgaaccgcg agacgttggc atgggccccg gcatccatcg
     3181 actggacccg ttcgagccgc accggcgcgt cccctggcca gaccgggatc tccggcgaga
     3241 tgccgaccga gatatcgtag atccgcatgg acggcggcgg attatagagt cgatcccaac
     3301 agaatgcgaa ccgggtcttt cccgcgacta taatcggccc atgcccacca attggcaggc
     3361 gatcgatcgc gcaattgatg accggctcga gagaactcta g
//
LOCUS       DATDPQ010000219         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2723828, whole genome shotgun sequence.
ACCESSION   DATDPQ010000219 DATDPQ010000000
VERSION     DATDPQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723828"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..522)
                     /locus_tag="VI520_04985"
     CDS             complement(133..522)
                     /locus_tag="VI520_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HLF37272.1"
                     /translation="MPRYDYRCQSCKKRSVIFQTYAEYGRTKVKCPHCGSEELQRLIG
                     RVRIARSEESRLDELSDPSDWGDVDENDPRSMARMMRKMGRELGEDMPGEFNEVVDRL
                     EAGEDPDDIEESMPDLGGENGGDDFDF"
     gene            566..1171
                     /locus_tag="VI520_04990"
     CDS             566..1171
                     /locus_tag="VI520_04990"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF011325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLF37273.1"
                     /translation="MNAPTTPSLLVVISGPSGVGKDTVVHHLKQRGLPFHFVVTATSR
                     PKRPDEVHGVDYFFYSEPEFLDLLERGELLEHALVYNEHKGVPKQQVRDAMASGLDVV
                     MRVDVQGAKTIKQLAPEAIAIFLMARDEAELAQRLRMRKTESEEHLQTRLQAAREELH
                     YLDLFDYRVVNADSEVEAAVSTILAIIEAEHHRTHPRQVTL"
     gene            1283..>2157
                     /locus_tag="VI520_04995"
     CDS             1283..>2157
                     /locus_tag="VI520_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLF37274.1"
                     /translation="MSQNEQPQVFDLTIIGAGPTGLFGAFYAGMRELSCKLIDALPQP
                     GGQITALYPEKKIYDTPGFVSIVGKDLVENLYQQAAQWGCTFVLGEQCDTLKRVPLPG
                     GQPEELCWVIGTSKADHYTRAVVIAAGIGAFRPVKLENESIDRFEDNGVVYFVRKIDP
                     YAGKRVLIVGGGDSAVDWALATDPIAASVTLIHRREGFRAHDLSVNKLKDSGVDVRLF
                     YELRELQGDGKLERAVIFDNRTNEETTLDIDATILALGFKADLGPLREWGLETVGRRY
                     LRVNNKMETNLPMVYA"
BASE COUNT          420 a          693 c          642 g          402 t
ORIGIN      
        1 ggtcgtgatc gatgcgactc gctccaaagc tgagatccaa tccgacatgc aggagcccgt
       61 actcacccga ctggaatccc aagctagcta agcgctgaag ctacgacggt cccgcagggt
      121 tcggcgtccg gctcagaaat cgaagtcgtc gccgccattc tcaccgccca gatccggcat
      181 gctctcctcg atgtcgtccg ggtcctcgcc ggcctccagc cggtcgacca cctcgttgaa
      241 ctcccctggc atgtcctccc ctagctccct ccccatcttg cgcatcatgc gggccatcga
      301 gcgcgggtcg ttttcgtcga cgtcgcccca gtcgctcgga tcggagagtt cgtccaacct
      361 ggactcctca gaacgggcaa tcctcacccg cccgatgagc cgctgcagct cttcgctgcc
      421 gcagtgcggg catttgacct tcgtccgccc atactcggcg taggtctgaa agattaccga
      481 tcgcttcttg cagctttggc agcggtagtc gtaacgcggc atcgcgtcct ctcgagcttc
      541 ggattatact caccgccccc tggccatgaa cgccccgaca acgccctctc tcttggtggt
      601 catctccggc ccctccggag tagggaaaga cactgtagtc catcatctca agcagcgcgg
      661 tttgccgttc cactttgtgg taaccgctac ttcccgaccc aagcggccgg atgaagtgca
      721 cggggtggat tacttcttct acagcgagcc ggagttcctc gacctgctgg aacgaggcga
      781 gctgcttgaa catgccctgg tctacaacga acacaaagga gtccccaagc agcaggtgcg
      841 ggatgcaatg gccagtgggt tggacgtggt tatgcgggtc gatgtgcaag gcgccaagac
      901 gattaagcag ctcgcccccg aggcgatcgc gatcttcctg atggctcgcg acgaggccga
      961 gctggcccag cggctgcgga tgcgcaagac cgagtccgaa gagcacctgc aaacccggct
     1021 gcaggctgcc cgggaggagc tccactacct ggatctcttc gattaccgcg tagtgaatgc
     1081 cgattcggag gtggaggcgg cggtcagcac catcctggcg atcattgagg ccgaacatca
     1141 ccgcacccat ccgcggcagg tgaccttgtg aggtcacacc gcgggcctat tcacccgcct
     1201 gcgaccgctc tgctacaatg aagcttgatt gtgaaggatg tcacggacaa cgccctcaga
     1261 tgacaggagc gacgcctgac ggatgagcca gaacgagcag ccccaggtct ttgatctcac
     1321 gatcattggc gccgggccga ccggcctgtt tggcgccttc tacgccggca tgcgcgagct
     1381 gagctgcaag ctgatcgacg cgcttcccca gccgggcggc cagatcacgg ccctgtatcc
     1441 ggagaagaag atctacgaca cccccggttt cgtctccatc gtcggcaagg atctggtcga
     1501 gaacctgtat caacaggcgg cccagtgggg ctgcacgttt gtgcttgggg agcaatgcga
     1561 taccctcaaa cgagttccgc tcccgggcgg tcagccggaa gaactgtgtt gggtcatcgg
     1621 aacctccaag gccgatcact acacccgggc ggtggtcatt gcagccggga tcggcgcctt
     1681 ccggccggtc aagctggaaa acgaaagcat cgaccgattt gaagataacg gagtggtcta
     1741 cttcgtccgg aaaatcgacc cgtacgccgg taagcgggta ttgatcgtcg gagggggcga
     1801 cagcgcggtg gactgggccc tggcgaccga tccgatcgcg gccagcgtta ctctgatcca
     1861 ccggcgcgag gggttccggg cccatgatct atcggtcaac aaactcaagg actcgggcgt
     1921 cgacgtcagg ttgttctacg aactgcgcga gctgcagggg gacgggaaac tggagcgggc
     1981 ggtgatcttc gacaaccgca ccaacgaaga aaccaccctc gatattgacg ccaccattct
     2041 ggcccttggc ttcaaagccg acctcggtcc gctccgcgag tggggcctgg agaccgtcgg
     2101 ccggcgctat ctccgggtca acaacaagat ggagaccaac ctaccgatgg tctacgc
//
LOCUS       DATDPQ010000220         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_885177, whole genome shotgun sequence.
ACCESSION   DATDPQ010000220 DATDPQ010000000
VERSION     DATDPQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885177"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /locus_tag="VI520_05000"
     CDS             <1..1720
                     /locus_tag="VI520_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37275.1"
                     /translation="FLIYNTMTFSVLRRRPMLGTLRAIGSTGDQILRMVLTEAAVLAL
                     LGSAIGIGLGFLMARGAVGLVSQTISDFYFLSAVQDMPLSPALALKGAGLGIGAGLLA
                     ALAPAIEAARVTPVEVWRRSSAETRATSRLLPLTAAGVGLASVGALGFRLSRSSLTWS
                     FAAMLAILAGIALLAPLATRAVVLAARAASRHLLVRIAAGSLPRHSSRTGPALAALTV
                     ALAVAVGVTLMIASFRSTVENWLNLTLQSDLYVGSPATSGTRPMASLSPQLATRLAAM
                     PGVEAVEAIRAVLVGSEFGEFHLSAVDPSRVRDADLYRLAHGSASEVWEQVRAGAVLV
                     SESLASRNPVGRSITLSTDRGAKPFPVAGVFYDYSTDRGTVLMSREVYLDNWDDPAIS
                     SVGITAEVGVEPAELAERVRVSLEGTGLEVRINGQVREQALAIFDRTFAITAALRLLA
                     VVVAFFGVLGALLALLLERSRELATLSALGLTPAGLARLVFIESGLMGLTASLLAMPT
                     GLLLARLLIEVIDERSFGWTIQLEWQWLPFVQAMIVGVGASLAAAVYPTARLRRLSLA
                     QALAQE"
     gene            1727..2860
                     /locus_tag="VI520_05005"
     CDS             1727..2860
                     /locus_tag="VI520_05005"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HLF37276.1"
                     /translation="MRRFALAAAEVAIGLAIALLLSGCQLEGESPPSLSLAPAAAGPD
                     FKDALEPLEFSSPRDHGPHFDFQTEWWYYTGNLESEIGDRYGYQLTLFRRGMTPGVAR
                     RPSRFGSNQIYFAHLALTDASLGEHRAFERFSRGVDGLAGASAEPYRVYLEDWSVAAV
                     GSAGSTLRLQAREQDIRLDLRLQAEQPLVAHGPGGLSPKGVESTDATYYYSFTALPTE
                     GTLWVRGEQLEVQGSSWFDHEWGSSVLPVGATGWDWFGLQLDDGRELMLYQVRTGQDV
                     LVGGGTLVGEDGRVVRLAAADFQIETLGTWSSRLSDSEYPSGWRIVVLDEQLELELRP
                     LLPDQENRLSIVYWEGAVDVIGSVSGRGYVELTGYQSSLQPWF"
     gene            complement(2872..>3053)
                     /locus_tag="VI520_05010"
     CDS             complement(2872..>3053)
                     /locus_tag="VI520_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37277.1"
                     /translation="VVGRANAQGTEPRSFLERAILTPSAEIVPGFQNLMPADFERRVS
                     PSDREALIAFLLTLE"
BASE COUNT          476 a          925 c         1035 g          617 t
ORIGIN      
        1 ttttctgatc tacaacacga tgaccttttc cgtccttcgc cggcgaccaa tgcttggaac
       61 cctgcgggcg atcggaagca ccggcgacca gatccttcga atggttctca cggaggcggc
      121 agtcctcgca ctgcttggtt cagcaatcgg aatcggcctt ggctttctca tggctcgtgg
      181 tgcagtcggc ctagtctcgc agacaatctc cgacttctac tttctcagcg cggtgcagga
      241 tatgccgctg agcccggccc tggcactcaa gggggcgggg ctggggatcg gcgccggatt
      301 gctggccgcg ttggcgccgg caatcgaggc cgccagagta accccggtgg aggtgtggcg
      361 ccgaagttcc gccgaaacgc gggctaccag caggttgctc ccgcttacgg ccgccggagt
      421 cgggctcgcc tcggttgggg cgcttggatt ccgactcagc cgatcgtcct tgacgtggag
      481 tttcgccgca atgctggcga tcctggccgg gatcgcccta ctggctccgc ttgccacccg
      541 ggcggtggtc ctggcggcgc gggccgcgtc acggcatctg ctggttcgga tcgcggcggg
      601 aagtctgccc cgccatagca gccgaaccgg tccggcgttg gccgccctga cggtggccct
      661 cgcagtagcg gtcggcgtga ctctgatgat cgccagtttt cgctcgacgg tcgagaactg
      721 gctgaacctg accttgcaat ccgatttgta cgtcggttct ccggccacca gcggaactcg
      781 accgatggcc tccctctctc cccagctcgc gacccggttg gctgcgatgc cgggggtgga
      841 ggctgttgaa gccatacggg cggtgttggt cggcagcgag ttcggggaat tccatctctc
      901 ggccgtcgac ccaagtcgag tgcgagatgc cgatctgtat aggctcgctc acggcagtgc
      961 ctcggaagtc tgggagcaag tgcgcgccgg tgcggtgctg gtgagcgaga gcctggccag
     1021 ccggaatcca gtcgggcgct cgatcacttt gagcaccgat cgcggggcga aaccattccc
     1081 agtggcaggt gtgttctacg actattcgac cgaccggggg acggttttga tgagtcgtga
     1141 agtctatttg gacaactggg acgacccggc gatctcgtcg gtcgggatta ccgccgaagt
     1201 tggggttgag ccggccgaac tcgccgagcg ggttcgagtt tccttggagg ggaccgggct
     1261 cgaagtacgg atcaacggtc aggtccgcga gcaggcgctg gcgattttcg atcggacttt
     1321 cgccatcact gctgccctgc ggctgctggc agtggtcgtt gcattctttg gagtgctcgg
     1381 cgcgctcctg gcgctcctgc tggaacgatc gcgcgaactc gcgaccttga gcgccctcgg
     1441 gctgacaccc gcggggctgg cgcggttggt gttcattgaa agcggactca tggggttgac
     1501 ggcttcactg ctggcaatgc cgactggatt gctcttggcc aggctgctca tcgaggtcat
     1561 cgatgagcga tcctttggat ggacgatcca gctcgagtgg cagtggcttc cttttgtcca
     1621 ggcgatgatc gtcggagtcg gggcctccct ggcggccgcg gtctacccga ccgcccgact
     1681 gcgccgactc tccctggctc aggcccttgc tcaggaatag accgcgatga gacgcttcgc
     1741 cctcgccgcc gccgaggtcg caatcggcct tgcaatcgct ttgctgctga gcggctgcca
     1801 actcgaaggt gagtcgccgc cctcgctcag cttggcccct gccgccgctg gcccagactt
     1861 caaggacgcc ctcgaacctc tggaattcag ttcgccccgc gatcacggcc cccacttcga
     1921 cttccaaacc gagtggtggt actacactgg caacctggaa tcggagatcg gcgatcgcta
     1981 tggctatcag ctgaccctgt tccgccgcgg gatgacgccg ggggtcgccc ggcgtccatc
     2041 tcggttcggc tcgaatcaaa tctacttcgc gcacttagcg ctgaccgacg cttccctcgg
     2101 ggagcatcgc gcctttgagc gattcagccg gggagtggac gggttggcgg gagccagcgc
     2161 cgagccatac cgggtgtact tggaagattg gtcggtcgcg gcggtcggtt cggcggggtc
     2221 aaccctccgg ctccaagccc gcgagcagga cattcgactc gatcttcgcc tgcaagccga
     2281 gcagccactg gtcgctcacg gaccgggcgg cctcagtccg aagggagtcg aatcaacgga
     2341 cgccacctac tactatagct tcacggcgtt gccgaccgag ggaaccctgt gggtgcgggg
     2401 agagcagctc gaggtccagg gctcgagctg gttcgatcat gagtggggaa gcagcgtgtt
     2461 acccgtcggc gctactggct gggactggtt tgggctgcag cttgacgatg gtcgagagct
     2521 tatgctctat caggtgcgaa ccggccagga tgttctcgtc ggcggaggaa ccttggttgg
     2581 ggaagacggc cgggttgtcc ggctggccgc ggcggatttt cagatcgaaa cgctggggac
     2641 ttggagcagc cgcctgtccg attcggagta tccgtccggt tggcggatcg tggtgctcga
     2701 cgaacagttg gagctcgaac tgcggcctct gttgccggat caggagaacc ggctgtcgat
     2761 cgtgtattgg gagggggccg tggacgtgat cggttcggta agcgggcgcg gctatgtcga
     2821 actgaccggt tatcagtcta gtcttcagcc gtggttttga tcgatcggcc gctactcgag
     2881 ggtaagcagg aaggcgatga gcgcctcccg atccgaaggg ctcacccggc gctcgaagtc
     2941 ggcaggcatc agattctgga agccgggcac gatttcggcc gagggagtga ggatggcccg
     3001 ttccaagaac gaccgaggct cggttccttg agcgttcgcc cggccgacca ccc
//
LOCUS       DATDPQ010000221         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_363097, whole genome shotgun sequence.
ACCESSION   DATDPQ010000221 DATDPQ010000000
VERSION     DATDPQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363097"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VI520_05015"
     CDS             <1..298
                     /locus_tag="VI520_05015"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HLF37278.1"
                     /translation="VEIDAELRNEGLAREFVRRVQELRKQAGFAVEDRIHIQYASTLE
                     LGTAVEAFRDYITAETLALSLDPVEHPAGDAQEDFAFDAQRVRLGLSQANPEEA"
     gene            387..908
                     /gene="lspA"
                     /locus_tag="VI520_05020"
     CDS             387..908
                     /gene="lspA"
                     /locus_tag="VI520_05020"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HLF37279.1"
                     /translation="MAKLARNYLGLVGISGFVVALDQWSKVLLRLRLAPGETWSPWEW
                     LAPYARLVHSDNTGAAFGIFQDGASFFTVVAILVSAAIIYYFPRVPVEQRALRIALAL
                     QLGGAVGNLIDRLLQAGRVTDLISIGSFPVFNLADASISVGVAILLGSMLLQKRRPTE
                     PEDSLAEIEQTPG"
     gene            941..1861
                     /locus_tag="VI520_05025"
     CDS             941..1861
                     /locus_tag="VI520_05025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLF37280.1"
                     /translation="MFGQESIDLRVKATDRLDRILVSQLPGLSRARLQALIRAGWVTV
                     NGRTVRKPGLSVGPEDVIQVAALPPAETRLRPQSIPLEILYEDDALFVINKPAGMVVH
                     PGAGHPQATLVHAALAHAPEVEAVGDASRPGIVHRLDKDTSGVMVLAKTNEALKHLQQ
                     QFRRREVEKLYLGLVDGAPPSPTGQIEAPLARSPRHRKRIAVVQAPGSRLAVTRFQTR
                     ERFPRHSLLELRPLTGRTHQIRVHLQFLGCPVVGDRVYGRKQPTLPVARQQLHAWRLT
                     FRLPGDSQPRTFEAAIPADLREAIELARRP"
     gene            1947..2990
                     /gene="ltaE"
                     /locus_tag="VI520_05030"
     CDS             1947..2990
                     /gene="ltaE"
                     /locus_tag="VI520_05030"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HLF37281.1"
                     /translation="MEWADFRSDTVTQPTTAMRAAMADAEVGDDVFGEDPTVKQLEEM
                     AAERMGMEAALFVASGTMGNLTAVLTHCHRGDEIIVGDRSHTFLHEAGGVSALGGIHP
                     HTVRNQSDGTLDPQEIIGAIRAVDDHFPTSRLIILENTHNHSGGTPLSADYIRQVGGL
                     AREHDLRLHMDGARIFNAAIALGSSPAELAAPVDSITFCLSKGLCAPVGSLLCGPAEF
                     IRQARRTRKQLGGGMRQAGILAAAGIVALESMVDRLAEDHQRARRLAAGLAGIPGVQV
                     EHDPPPTNMVYFRLEPQSGVTTQDLVQALASRGVRLTARNPFRMRMVVHYWIDDDAVE
                     RALEGISAAMSGP"
     gene            3022..3095
                     /locus_tag="VI520_05035"
     tRNA            3022..3095
                     /locus_tag="VI520_05035"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3056..3058,aa:Asp,seq:gtc)
     gene            3263..>3914
                     /locus_tag="VI520_05040"
     CDS             3263..>3914
                     /locus_tag="VI520_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLF37282.1"
                     /translation="MTSGDVKYWIGFNLVSGIGPVRVRSLVQTFGSIEAAWNAPAEEL
                     RRVGLGEKLVQRLVEARSALDLAKELGRVQQLGLQVLPMDDPQYPARLKEIESPPLVL
                     YLSGQLGPQDEWAAAIVGTRRATPYGRAVTAELAGGLAASGVTVVSGLARGIDGIAHQ
                     AALDAGGRTLAVLGSGLDHIYPPEHRNLAGTIASSGAVLSDYPLGTSPEGGNFPARN"
BASE COUNT          687 a         1219 c         1287 g          721 t
ORIGIN      
        1 agtcgaaatc gatgccgaac tgcgtaacga ggggctggcc cgggagtttg ttcgcagggt
       61 ccaggaactt cgcaaacagg ccggcttcgc agtcgaagac cggattcaca tccagtacgc
      121 ttcgaccctc gagctgggca cggcggtgga ggctttccgc gattacatca ccgccgaaac
      181 cctggccctc agcctggatc cggttgagca ccccgccggg gacgcgcagg aggacttcgc
      241 gttcgacgcc cagcgggtga ggctggggtt gtcgcaggcc aacccagaag aagcttgatc
      301 ggggccctgc gagccggcgg gccgcgggtc tccgccgcct aaagttcggt taggatacaa
      361 tcgacagctt tcgggttcag gagcccatgg cgaagttggc acggaactat ctggggctgg
      421 ttgggattag cgggttcgtc gtcgcgttgg atcagtggag caaggttttg ctccgcctgc
      481 ggctggcacc cggggagacc tggtcgccct gggagtggct tgccccgtat gcccggctgg
      541 tccattccga caacaccggg gcagccttcg ggatcttcca agatggcgcg tcgttcttca
      601 ccgtcgtggc gatcctggtg tccgccgcga tcatctacta cttcccgcgg gtgccggtcg
      661 aacaacgagc cttgcggatc gcgctggccc ttcaactcgg cggggcggtg ggcaacctga
      721 tcgaccggct cttgcaggcc ggccgggtga ccgatctgat ttcgatcggc agttttccgg
      781 ttttcaacct ggccgacgcc agtatctccg tcggggtggc gatcctcttg ggctcaatgc
      841 tgctgcagaa gcgccggccg acggaacccg aggactcgct cgccgaaatc gagcagactc
      901 ccggctagcc cacgaggcac gactcgccaa ttgcgcagcc atgtttgggc aagaatcaat
      961 cgacttgagg gtcaaggcca ccgatcggct cgatcggata ttggtttcgc agttgccggg
     1021 tttgtcacgg gcaagactgc aagccctgat ccgggccggc tgggtcacgg tcaatggtcg
     1081 caccgtacga aagccggggc tttcagtcgg gccggaggac gtcatccagg tcgctgcgct
     1141 gccgccagcc gagacccggc tccgccctca gtccatcccg ctcgaaatct tgtatgagga
     1201 cgacgccctc tttgtgatca acaagccggc ggggatggtc gtccatcccg gcgccggcca
     1261 cccgcaagcc accctggtgc acgccgcatt agcccacgcc cccgaagttg aagcggtcgg
     1321 cgatgcgagt cggcctggaa tcgtgcatcg cctggataag gacacctccg gggtgatggt
     1381 tttagcgaag accaatgagg cacttaagca tctgcagcag cagtttcgac gccgggaggt
     1441 cgagaaactc tacctgggat tagtcgacgg tgcccctccg agtccaaccg gacaaatcga
     1501 agctccgctc gcccggtccc cgcgccatcg aaagcggatc gccgtggttc aggctcctgg
     1561 atcgaggctg gcggtcaccc ggtttcaaac ccgcgaacga tttccccgcc acagcttgct
     1621 ggagctcagg ccgctcaccg ggcggaccca tcaaatccgg gtccatctgc agtttctggg
     1681 ctgcccggtc gtcggggacc gggtgtacgg ccgcaagcaa cccaccctcc cggtcgcccg
     1741 gcagcagttg cacgcctggc ggctgacctt tcgactgccc ggtgacagcc agccccgaac
     1801 atttgaagct gccatcccgg ccgacctgcg ggaggcgatc gagcttgctc ggcggccatg
     1861 agccttgcgt ctcacctgcg aggtgcgcat agccaagcac aggagttggg tctgccgccc
     1921 aactttcgaa ccgtggaggg caactagtgg agtgggctga ctttcgttcg gacactgtca
     1981 ctcagccgac caccgccatg cgcgccgcga tggccgacgc ggaagtaggg gacgatgtct
     2041 tcggggagga ccccacagtt aagcagctcg aggagatggc tgcagaacgg atggggatgg
     2101 aggcggccct gtttgtcgcc tccggcacga tgggcaatct gaccgccgtg ctgacccatt
     2161 gccatcgggg ggacgagatc atcgtggggg accgttcgca caccttcctg cacgaagcag
     2221 gcggagtttc ggcactaggg ggcatccacc ctcatacggt gcgtaaccag agcgatggaa
     2281 cgctggaccc acaggaaatc attggcgcga ttcgggctgt ggacgaccac tttcctactt
     2341 caaggctgat cattctggaa aacacgcaca accattctgg gggaaccccg ctcagtgccg
     2401 attacattcg tcaggtgggc ggcttggcgc gggaacacga tctgcggctg cacatggacg
     2461 gcgctcgaat cttcaatgct gctatcgccc tcggcagctc gccggccgaa ctcgccgcac
     2521 cggtcgactc aatcaccttc tgtctctcga aaggactgtg tgccccggtc gggtcgctgc
     2581 tgtgtggacc cgccgaattc atccgccagg ctcgacggac ccgcaaacag ctgggaggtg
     2641 ggatgcggca ggccggaatc ctggcggcgg ccggaatagt tgcattggaa agtatggtcg
     2701 atcgattggc agaggaccac caacgggccc gtcggttggc ggcggggcta gccggcatcc
     2761 ccggcgtcca ggtggagcac gatccgccgc cgaccaatat ggtgtacttc cgcctcgagc
     2821 cacagagcgg cgtgacgacc caggatctcg tccaggcgct agcttcgcgg ggcgtaagac
     2881 ttaccgctcg caatccgttt cgaatgcgga tggtcgtgca ctattggatc gatgacgatg
     2941 ccgttgaacg cgccctcgag ggaatctcag ctgctatgtc cggcccgtaa accacgccgt
     3001 gtggtagact agccggggct cgggggtgta gtgtagcggc caaacacgct gccctgtcaa
     3061 ggcagagatc gcgggttcga atcccgtcat ccccgctgca gatttcttcc cgaacctgct
     3121 cctcaaacca atcctgacag ccagagcctg accggccact accgccgtgc ctgacttcgc
     3181 ctccgagcgg gcgggagggg ggcttgcttg actttcagaa aatcgctatg gtatgctccg
     3241 ccggctctgg gccgagctta tgatgacttc gggcgacgtc aaatactgga ttgggttcaa
     3301 cctggtctcc ggaatcggcc cggtccgagt tcgctcgctt gttcagacct ttggcagtat
     3361 cgaggccgct tggaacgcgc cggcggaaga gctgcgcagg gtcggccttg gagagaaact
     3421 ggtgcagcgg ctggtcgagg cgagatcggc tcttgatctt gcgaaggagc ttggccgagt
     3481 gcagcagttg gggctgcagg tcctgccgat ggacgatccg caatacccag cgcggctgaa
     3541 ggaaattgag tcgccgccgc tagttctcta cctgtcgggg cagctcgggc cccaagacga
     3601 gtgggctgcg gcgatcgtcg ggacccggcg ggcgacgcct tacggcaggg cggtcactgc
     3661 ggagttggct ggcggcctgg cggccagcgg cgtgacggtg gtctcgggtc tggcacgtgg
     3721 gatcgacggg attgcccacc aggccgcgct ggacgctgga gggcggaccc tggcggtgct
     3781 cggttcgggc ttggaccaca tttaccctcc agagcatcgc aaccttgctg ggacgatcgc
     3841 cagctccggc gcggtgctga gcgactaccc gcttggaacg agcccggagg gaggcaactt
     3901 tcctgcccgc aatc
//
LOCUS       DATDPQ010000222         6784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3775127, whole genome shotgun sequence.
ACCESSION   DATDPQ010000222 DATDPQ010000000
VERSION     DATDPQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3775127"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2730
                     /locus_tag="VI520_05045"
     CDS             <1..2730
                     /locus_tag="VI520_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HLF37283.1"
                     /translation="YLDPAFFTTDHVTFLQLIDTDTGWANILAATINDTDDTLYLWND
                     LAGEAYGFMATNPITRGVWHTIEVQATMSTGPATNDGSARLWMDGVLEIQATGINLYG
                     TGIDTIDRFATVFYWANPKTEANTLYVDDAQLRAYVNTEPTTGLGSEQPSSCGPTATP
                     TPTSTATPTSTSTPTPTPTSGGGLVAHWAFDEGSGQTAADSSGTGNTGTLGTSGGVES
                     ADPTWTCVSGGNTLNFDGIDDQVLAPDYDVLNTISISTWIKWDVLGASEGIISKRTDA
                     EVAGNWALRGDNSTPGLLEWMVWSGLDSSQSLNTSTTITPGVWTHVVLTFNDSTNAAN
                     FYINGSSNSSGSITNNLANTPQPIVIGWSGQNVQFFDGSIEDMRIYNRVLTPAEISAL
                     AASPPADCATSPTWWDANYGYRARITVSAGSSAIAANYPTRFTFNHAGLVSGGKSLAS
                     GNDARILYWNGSTWTELSRVLFNDGPAGQASSTWNAASTTLFFKTVAGISASGSDTGY
                     YLYYGNPLAGSPPTNTPSSRYYLAESLGETTTTSSSYATKLQLQFTPSAATEHWVVVA
                     TWRQREIGSLGTTVSGGYGRISINGSPRTGTNDITFRQSGDVWKTFQAFLKVTGTTAQ
                     QTISIDHAWNGGGTDAIDNARILAFLIPDPASADIQYGEALAVTNDTANPTNSLTTTF
                     SPSSAGDYVWMANGFVHEGPGGGSNGGLFALDETAATQQNSAESYTGVGSGFVPFLHF
                     ERRNLTTGSKSFIIRHQPDAPTGGEREGLTQLLFRADVFEAVELASSTGLSSTTSTSP
                     VTKNTLTTAAPAARDYVYLVVMGLDDTVQNVTLSTFGEVRLGGTTMLAEEVAIDRLGY
                     DSQIGWAYAENVTGGRIIDARFWAETGNTSEAQWSHIVALRYKEPGTSLGPEE"
     gene            2765..4612
                     /locus_tag="VI520_05050"
     CDS             2765..4612
                     /locus_tag="VI520_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF37284.1"
                     /translation="MADLRILLCDDDPDLLGLLTRRLEKMGLEHDVAADGRLAISLVE
                     KNSYDVIVTDIYMPEATGLEVLQFAKQRDPDIQVMIITSSATLDNAIDALNHGAFGYL
                     TKPFDHLIVFDNMVSRAAEYRQLLVANKHKAEAQRRRGDMLEDEVAERVQQIQKKQKG
                     LLDLLGSLPDGILVVEEGGKVVLSSPVGERWLARDGKSEHQPIHAFISQVNAPAAERS
                     AKVRVEEHEFQLIAVDFPADGEMRRKAVIIREIESDGIGAGSLVTETVMGIKKGLAAL
                     YKQGVGAEAVRNLANQFEVLEQLAGWTKAEGGIAASPSASQGAVSPIKSISSVAQPVE
                     ELEGPKLNTAELADALRAAALADIEPNHAQPAAELLHSAEPSSEPSSDLAAHGFPSDE
                     AQEVDPAPQAIPPAAGKGPSAPETPSVSEPAAEAAATVSEQTFPMAQPGVLDPVARPI
                     DTGLFRPRIIPEAMNAKLGEPPPIQPTNGKRGPIRIGSSAANTQIFRRVLEKMSGETV
                     IGPEEPEPAAAAAEPRSRSRDLPAAEVGQLEPALDPVAALRELAVAAASDFGAEVAAE
                     PVVPPKQARAAQAKELEPKPASAKRATRSWPPKLPSNDPEWDDALETKG"
     gene            complement(4715..5752)
                     /locus_tag="VI520_05055"
     CDS             complement(4715..5752)
                     /locus_tag="VI520_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HLF37285.1"
                     /translation="MNVAAFVRGIATLFWVVAIALIGLSVARAARGQPFKGATSLIII
                     SVIVALAATSVGTSLIFVQPTERGVVISALQGGVRPTALEPGLQFVVPFLESVIPYTI
                     TRQTYTMSIAPTEGQIAGDDSVEARTSDGQVVKVDASVIFSIDPGRVVATHIQWQGRY
                     LDNLVRPKARGVIRDAVSQFGIEEVYSSQRFVLAERMRAELETSLVEGGLILHDFVLR
                     NIAFSPEYAASVEQKQIAEQTAQQAAFVVEQRTQEAEQARQVAKGVADSVVIAAQGQA
                     EARLIQAEAEARALLLIADALRNNPDLLVFEYIQKLAPGIQVMLVPSNNPFLLPLPSL
                     TPAAGTEATVP"
     gene            complement(5769..6248)
                     /gene="dtd"
                     /locus_tag="VI520_05060"
     CDS             complement(5769..6248)
                     /gene="dtd"
                     /locus_tag="VI520_05060"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HLF37286.1"
                     /translation="MRALVQRVRSGGVRVDGKTLAQIGKGLVILLGVGEGDTEMGARW
                     LANKCAHLRVFADDQGKMNRSLLEVQGEAIVVSQFTLYGDVRKGRRPSFVHAASPDIA
                     EPLVERFAALLREEGVPTQTGSFGAMMLVEIENDGPVTLILEREPGQTQEPEPGTTR"
     gene            complement(6253..6450)
                     /locus_tag="VI520_05065"
                     /pseudo
     CDS             complement(6253..6450)
                     /locus_tag="VI520_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
BASE COUNT         1314 a         2225 c         2028 g         1217 t
ORIGIN      
        1 tacctggatc cggccttctt caccaccgac cacgtcacct tcctgcagct gatcgacacc
       61 gataccggct gggccaacat cctggctgcc accatcaatg acaccgacga cacgctttat
      121 ctgtggaacg acctggccgg cgaggcctac gggttcatgg ccaccaaccc catcacccgc
      181 ggcgtctggc acaccatcga agtacaggcc accatgtcca ccggtccggc gaccaacgat
      241 gggtctgctc gcctctggat ggacggtgtc ctggagatcc aggccaccgg catcaacctg
      301 tacggcaccg gcatcgacac gatcgaccgc tttgccaccg tcttctactg ggccaacccg
      361 aagaccgaag cgaacaccct ctacgtcgat gacgcccagc tgcgggctta tgtgaacacc
      421 gaacccacca ccggcttggg ttccgagcag ccctccagct gcggccccac cgccaccccg
      481 acccccactt ctaccgcgac gccgaccagt acctcgaccc ccaccccaac accgacaagt
      541 ggtggaggac tcgtcgcgca ctgggccttt gacgagggga gtggccagac cgcggccgac
      601 tcttccggaa ccggcaacac gggaacgctt ggaaccagcg gtggggtgga aagcgccgac
      661 ccgacgtgga cctgcgtctc cggcggcaac acgttgaact tcgacgggat cgacgaccag
      721 gtgttggcgc ctgactacga tgtactgaat acgatctcga tctctacatg gatcaaatgg
      781 gacgtgctgg gggcgagcga aggcatcatt tcgaagcgta ctgacgccga ggtcgccggg
      841 aactgggcgc tccgaggaga caactccacc cccggtctgc tggaatggat ggtgtggagc
      901 ggtttggaca gctcacaatc tctcaatacg tccaccacca ttacccccgg cgtgtggacc
      961 catgtggtct tgaccttcaa cgattcgacg aatgcggcga acttctacat caatgggagc
     1021 tcgaatagca gcggctcaat caccaacaac ttggccaaca cgccgcaacc gattgtgatc
     1081 ggttggtctg gacagaacgt ccagttcttt gatggcagca tcgaggacat gcgcatctac
     1141 aaccgcgtct tgaccccggc cgagatcagc gcattggccg cctccccgcc ggccgactgt
     1201 gcaacctcac cgacctggtg ggacgcgaac tatggctacc gggcgcggat cacggtctcg
     1261 gccggctcct cggccatcgc ggccaactac cccactcgct tcactttcaa ccatgccgga
     1321 ttggtgagcg gcggcaagtc gctggccagc ggcaatgacg cccgcatcct ctactggaac
     1381 ggctcaacct ggactgagct gtcgcgggtc ctcttcaacg acggccccgc cggtcaggcc
     1441 agctcgacct ggaacgcggc cagcaccacc ctcttcttca agaccgtggc cggcatttcc
     1501 gccagcggat cggacaccgg ctactacctg tactacggca acccgctggc cggcagcccg
     1561 cccaccaaca ccccctcctc ccgctactac ctggcggaaa gcctgggcga gaccaccacg
     1621 accagttcgt cctacgcgac caagctgcag ctgcagttca cccccagcgc ggccactgaa
     1681 cactgggtgg tggtcgccac ctggcgccag cgtgaaattg ggagccttgg tacgaccgtc
     1741 tccggaggct acggaaggat ctccattaac ggctcgccac ggaccggcac caatgacatt
     1801 acattccggc agtcgggaga cgtctggaag actttccagg ccttccttaa ggtaaccggg
     1861 accacagccc agcagacgat ctcgatcgac catgcctgga acggcggggg aaccgacgcg
     1921 attgacaacg ctcggatcct ggccttcctg atccccgacc cagccagcgc ggacatccag
     1981 tacggcgagg ccctggccgt caccaacgac acggcgaacc ccaccaattc cctgaccacc
     2041 accttctcac catccagtgc gggagactat gtctggatgg cgaacggctt tgtgcacgag
     2101 ggtccgggcg gcggttccaa cggcgggctg tttgccctcg atgaaactgc tgcgacccag
     2161 cagaactcgg ccgagtcgta taccggggtg ggatcgggtt tcgtgccctt cctccatttc
     2221 gagcgccgga acctcaccac cggcagcaag agcttcatca tccggcatca gccggatgct
     2281 cccaccggcg gtgaacgaga aggcttgacc cagctactct tccgcgccga tgtcttcgag
     2341 gcggtggaat tggccagctc cactggcctg agcagcacga ccagcaccag cccggtcacc
     2401 aagaacaccc tcaccaccgc ggctcccgcg gcccgtgact acgtctatct ggtcgtgatg
     2461 gggctggacg atactgtaca gaacgttacc ctctcgacct tcggggaagt caggctcggt
     2521 ggcacgacca tgttggcgga agaggtcgcg atcgatcgcc tcgggtacga ctcccagatc
     2581 ggctgggcgt atgccgaaaa cgtcacgggt ggccgcatca ttgatgcccg cttttgggcg
     2641 gagacaggga atacttcgga agcccagtgg tcccacatcg ttgccctgcg ctacaaggag
     2701 ccgggcacca gcttggggcc ggaggagtga gtggaggacc tctgattctt gaggccgaaa
     2761 tgcaatggct gacctaagaa ttttgctctg cgatgacgat cctgacctgt tggggttgct
     2821 gacccggcgg ctggagaaga tgggtctcga gcacgatgtc gcggccgatg gccggctggc
     2881 catttcactg gtcgagaaga acagctatga cgttatcgtg accgatatct acatgccgga
     2941 ggccaccggc ttggaagttc tgcagttcgc gaagcagcgc gatcctgata tccaggtgat
     3001 gatcatcacc tcttccgcca ccctcgacaa tgccatcgat gccctgaacc atggcgcctt
     3061 cggctatctc accaagccgt ttgatcactt gatcgtgttt gacaacatgg tctcgcgggc
     3121 cgcggagtac cgccagcttc tggtcgccaa caaacataag gccgaggctc agcggcggcg
     3181 gggggacatg ctggaggacg aagttgcaga gcgggttcaa cagattcaga agaagcagaa
     3241 aggtctgctg gacctcttgg ggtcgttgcc tgacgggatt ctggtggtcg aagagggcgg
     3301 caaggtcgtg ctcagcagtc cggtcggcga gcgctggttg gcccgggatg gaaagtctga
     3361 acatcagccc attcacgcgt ttatttccca ggtgaatgct ccggcagcgg agcgcagcgc
     3421 caaggtgcgg gttgaggagc acgaatttca actgattgcc gtggatttcc cggcggacgg
     3481 cgagatgcgg cgaaaggccg tgatcatccg tgagattgaa tccgatggga tcggcgccgg
     3541 aagtttggtg accgagactg tcatgggcat taagaagggc ctcgccgcgc tatacaagca
     3601 gggggtgggt gctgaggctg tccgaaatct ggctaaccag tttgaggtgc tcgagcagct
     3661 ggcgggttgg acgaaggcgg aggggggtat tgcggccagc ccgtccgcaa gccagggagc
     3721 cgttagccca atcaagagta tcagcagcgt tgcgcaaccg gtcgaagaac tggaggggcc
     3781 caagctgaac acggccgagt tggcagacgc gctccgggcg gcggccctag cggatatcga
     3841 gccgaaccat gcccaacctg ccgccgaatt gctgcactcc gcagagcctt cgtccgaacc
     3901 ctcgtccgac ctcgccgccc acggattccc ctcagacgaa gctcaggagg tggacccggc
     3961 cccccaagcg attccgcccg ccgccggcaa gggaccctcc gcgcctgaaa caccgtccgt
     4021 gtccgagccg gcagcggaag ctgcggcgac agtttctgag cagaccttcc ccatggcgca
     4081 gcccggtgta ttggacccgg tcgccaggcc gattgatacg gggctcttcc ggccccggat
     4141 cattccggag gcgatgaacg ctaagcttgg cgagccccct cctattcagc cgacaaacgg
     4201 caagcgcggc ccgatccgaa tcggatcttc ggccgccaat acccagattt tccggcgggt
     4261 gctggagaag atgtccggcg agaccgtgat cggaccggag gagcccgaac cggccgcggc
     4321 tgccgccgaa ccacggtctc gaagccgcga cctgcctgca gccgaggtcg ggcagctgga
     4381 gcctgccctg gatccggtgg ctgctctccg ggagctggcg gtcgcggcgg cgtctgactt
     4441 cggcgcggag gtcgcggcgg aaccggttgt cccaccgaag caagcccgag ccgcccaggc
     4501 gaaagaactc gaacccaaac cggcatcggc caagcgggct acaagaagct ggccgccgaa
     4561 gttgccctcg aatgacccgg agtgggacga cgccctcgaa accaagggtt gatcactgag
     4621 atgcaaggct gcgcccggtc agctgaccgg gcgctttgtt ttgttgacct caggatccta
     4681 tcgcgagttt acacccgatc ctggcgctgt tcgcttacgg caccgtcgcc tcggtacctg
     4741 ctgcaggtgt cagcgaggga agcggcagaa ggaacgggtt gttgctggga accagcatta
     4801 cctggattcc tggcgccagt ttctggatgt attcaaagac gagcaggtcg gggttattgc
     4861 gcagcgcgtc ggcgatcagc aacagcgccc tggcctcagc ctccgcctgg atcaatcggg
     4921 cctcagcctg cccttgggcg gcgatcacca ccgaatcggc cacccccttg gcaacttgcc
     4981 gggcttgctc cgcctcctgt gtccgctgct caaccacaaa tgccgcctgc tgagccgtct
     5041 gctcggcgat ctgcttctgc tccaccgaag cggcgtactc gggcgagaag gcgatattac
     5101 gcagcacgaa gtcatgcaga atcaagccac cctccaccag cgaagtctcc aattcggcgc
     5161 gcatccgctc ggccaggaca aaccgctgcg aactgtagac ctcctcgatg ccgaactgcg
     5221 agaccgcatc ccggatcacg ccgcgggcct tgggccggac caggttgtca agataccggc
     5281 cctgccactg gatatgggtc gccaccactc gaccagggtc gatggagaaa atcactgagg
     5341 cgtccacctt cacgacctgt ccgtcggagg ttcgggcctc gaccgagtca tctcccgcga
     5401 tttggccctc cgtcggcgcg attgacatgg tgtatgtctg gcgggtgatg gtgtacggga
     5461 tcaccgactc cagaaagggg acgacgaatt gcaagcctgg ctcaagcgcg gtcggacgca
     5521 ctcccccctg gagcgccgag atcactaccc cgcgctcggt gggctgtacg aagatcaagc
     5581 tggtcccgac tgaggtagcg gccagagcca cgatcaccga tatgatgatc aaactggtcg
     5641 cacccttgaa aggttgtccc cgggcggccc gggccacgga gagcccgatt agtgcgatcg
     5701 ccaccaccca gaagagagtg gcaattcctc gaacgaacgc tgcaacattc atctgctcct
     5761 cctcgaattc agcgagttgt gcctggttcc ggttcttgcg tttggcccgg ctcccgctcc
     5821 aatatcaagg tcaccggccc atcgttctcg atctcaacca gcatcatcgc cccgaaggac
     5881 cccgtctggg ttgggacacc ctcctcccgc aacagcgccg caaagcgctc gaccagcggc
     5941 tcggcgatgt ccggtgaggc ggcgtgtacg aagctgggac gccgtccttt tcggacatct
     6001 ccgtacaggg tgaattgtga gacgacaatg gcctcgccct gtacttccaa cagcgagcgg
     6061 ttcatcttgc cttggtcgtc agcaaacact cgcaagtggg cgcatttgtt ggccagccag
     6121 cgggctccca tctcggtgtc cccttcccca acccccagca ggataaccag accttttccg
     6181 atttgcgcca gggtctttcc gtcaaccctc acgcccccgc ttcgcacccg ctggacgagc
     6241 gcccgcatcg cttcagcttg ctctggtcgg gcgcccaccg accaggtccg caacctcccc
     6301 ggtattggcg tgccggcggg tctgcagctt cgagtagatc agctcgccgt ccaccgtcac
     6361 ctcaaacctt ccaccctccg aagggatgag ccggatcgat tgaatgtccc cctcgaattg
     6421 cttcgtcagc tcggccatca gaccgatggc tcgctcggtg tagtgtcaga ccacacagta
     6481 ttcgatggtg atgtccacca tggacctccg ggaacaattc tgcccatcga ttctactatg
     6541 cgcccccgcg gttaagggtg gtcgacgggg gatcctttct gcggcctccc tatgggataa
     6601 tcaaacggta cggaggtgga gaatgcgtcg gttctttaca ttcctgcttg gaaccgtgag
     6661 cggggctatc gtgggcgcct cggtcgcact gctcatcgct ccggcttcgg gtgaagagct
     6721 acggactcgg gcccggcagc gatttgctgg actgcgggac gagatccagg atgcctacga
     6781 gacc
//
LOCUS       DATDPQ010000223         7483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1679222, whole genome shotgun sequence.
ACCESSION   DATDPQ010000223 DATDPQ010000000
VERSION     DATDPQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679222"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1032)
                     /gene="pdhA"
                     /locus_tag="VI520_05070"
     CDS             complement(43..1032)
                     /gene="pdhA"
                     /locus_tag="VI520_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HLF37287.1"
                     /translation="MAQIDVAIKALDEKEMLRLYHEMVLVRRLEEEAARLYQQGKIGG
                     FLHLYIGQEAVSSGVLSVRLPKDRVLTAYRDHGVAIACGIEAKVVMAELLGKSTGAAK
                     GKGGSMHLADVDKNFWGGYAIVGGHLPLAVGMALADQYRGDDAVTICMFGDGATNIGY
                     FHESLNLSMVWKLPVLWICENNKYGMGTSVERSSAVLEMRQKAEGYAMPNSSVNGMDV
                     LAVRAAAQDALATVRESSTPYFLEIVTYRYRGHSMGDPERYRNSEEIERMRKDDPIAL
                     FRSQLLAEGHFDAESLDEEDGRVEKEILAAIEFAESSPDPVPADLYTDIYTED"
     gene            complement(1135..2460)
                     /locus_tag="VI520_05075"
     CDS             complement(1135..2460)
                     /locus_tag="VI520_05075"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HLF37288.1"
                     /translation="MTVWIHSIRLFALLLVSACAAQVGNGQPSDFQSPPATTPVSATA
                     TATALPSLEFNSIVLLAGEAPGDWEVIGMLRNPAEVGVSNVRIQVTVADSHGNQLAEL
                     TTGALMSNFAPGEAGPFIAEFPGVGPAVTAEARVDSFERHFIRRSKLEIEVLEEFLTE
                     TGELALLGTVTNPSSQVQSFVSLGLIGFGGASQPRAVAVLQHGPRVLGAEESVPFLAL
                     ARGNPGQLNWKAYHDAVEVGARVADSLVLDEAPRVRVDSQGNVFVVGSLRNAAGAPAQ
                     ASVLIMLWKGDRILSLTELRAPVPLQAGERLAFATQDFPGLGRRLAGSEPASLRIESR
                     IEGADPAVQPIVLQAEVEAFHSVGSSLFLRGILRNETLQAVDPAAVLAELRSTDGELL
                     SAGWFTTPGPLASGEQTEFVIRLPLPTGTDPGDVEFDLRALGSPLKAEE"
     gene            complement(2493..3962)
                     /locus_tag="VI520_05080"
     CDS             complement(2493..3962)
                     /locus_tag="VI520_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37289.1"
                     /translation="MIRNRVWIGLALIFLPVVGRTLWYHRGFYRPPAPIQLPAYSEFS
                     PPLPPVGEAAIELPEATAAPEGPLAGPPKVVLFDRSHSNRFSMPELRSLTAALSSQGG
                     RIEVLDSSFAFDELGLEGRLKYASSYVVVAPSVVFSPSEVTAVERFVEHGGRLLVVLD
                     PTRTGSGFDIFGFLILSASEDVSTANQLLESFNLAFSDDYLYNLMDNEGNFRNVLLRN
                     FGEHPLTAGLQTVALYAARSVQTEAGTLLLSGDSATLSSLTDTGGGLAAAALDPGSQA
                     LALGDLTFMTPPYDKVADNARLIQNLAEFLLDGDRRPDLHNLPFVFSGPVSVVQSADF
                     ALQAETLVALQGMSTALLPAGVSLSFPEEVPAEGDLLVLGLFEPDPELGELIEPLGLV
                     LPAEGSDGRLAAPGFGSFNPAGIGFVGLARSEARTTLVLLASDQAGLVSLLESVGQGL
                     LSDCLLSDTSALCQVGEGAGLGSSEPLEFDLSFEETFNQ"
     gene            complement(3959..4819)
                     /locus_tag="VI520_05085"
     CDS             complement(3959..4819)
                     /locus_tag="VI520_05085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HLF37290.1"
                     /translation="MTSPEPDPYKRGLRRWLAWIFLPLLVGLGLAALVPRPIVGLIYL
                     QEAIHAFSSRDMIAQIHFARDHPEVRAVVLVLDSPGGTVVDTEAVYLELAGLRERKPV
                     VTMAQGLAASGAYYLSVGTDYIFAGPSSLVGNVGVISQLPTAPSVFEDLASTGPYKLF
                     GSARDTRLREMEAIKQAFYSAVQLGRGEALAIGPERILRGEIWLGSEAARFGLVDELG
                     SISAAIEKAAELSHVANYRVADLRELAGLPSVLSVPFFFETPEGALTPYPKAPGFYLL
                     YVPPGERSLP"
     gene            complement(4941..6089)
                     /gene="moeB"
                     /locus_tag="VI520_05090"
     CDS             complement(4941..6089)
                     /gene="moeB"
                     /locus_tag="VI520_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HLF37291.1"
                     /translation="MELTHEEILRYSRHLLMPEVGLEGQRRLKAASALIIGTGGLGSP
                     VAMYLAAAGVGRLGLVDYDVVDFTNLQRQILHGSSTLGERKVISARARVLDINPEIKV
                     DIYDEPFTSENAMRIAEPYEILIDGTDNFPTRYLVNDLAVLTGKPNVYGSIFRFEGQL
                     SVFWAERGPCYRCLFPEPPPPGLVPSCAEGGVFGVLPGTIGALQATEALKLLLGIGEP
                     LIGKLLLFDALNMSFEELRLKKNPGCRVCSENPEVTELIDYEAFCGVPGHDREESLLA
                     SEFEIEPTDLAARLANGERFRLIDVREPHELEISRIEGAVLIPLGSLAARMHELDSSE
                     EMVVFCKSGSRSARAVELLAGAGFRKQKNLKGGINAWARQVDPSLPLY"
     gene            complement(6109..6558)
                     /locus_tag="VI520_05095"
     CDS             complement(6109..6558)
                     /locus_tag="VI520_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLF37292.1"
                     /translation="MSLDRLALALALILLGYLTYRAYGWAVLRRRARHGLGIDEYRPG
                     RPAILYFTDPGCAPCLSVQDPALQEVAAQFGEGLQIIKVRALERPELTDAWGVLSLPT
                     TFIIDSGGRPRGVNHGVARAPHLLDQLAAIGELGAAHPASPTPAASD"
     gene            complement(6560..7030)
                     /locus_tag="VI520_05100"
     CDS             complement(6560..7030)
                     /locus_tag="VI520_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HLF37293.1"
                     /translation="MTTLKAVDHSSLKTSMAVRLGFLLAIFITDQSWLVPILAALILI
                     GAARGKPEFTFVYRALRKANWISPELIQDNPEPHRFALGVGGTFLAGATMAFGSGSPA
                     LGWVLTWIVIALVGLNLFGGFCVGCAVYYWSSRVGVPGFSKAPPPGVFPGRRPG"
     gene            complement(7037..7309)
                     /locus_tag="VI520_05105"
     CDS             complement(7037..7309)
                     /locus_tag="VI520_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HLF37294.1"
                     /translation="MSHIRVPTALRPYTDGQIEVPTEAATVGGALQELAQRYPGLAPH
                     LFGDEGQLRPYVNLFVNDQDVRALDGVHTPLAESDQLMILPSVAGG"
BASE COUNT         1479 a         2335 c         2389 g         1280 t
ORIGIN      
        1 gagggcttgc gagattgcct cccggatggt gatctcaggc attcagtctt ccgtgtagat
       61 gtcggtatag aggtcggcag gcaccgggtc cgggctgctc tcggcgaatt cgatcgcggc
      121 caggatttcc ttttcgaccc ggccatcttc ctcgtcgagg gattctgcat cgaaatgtcc
      181 ctcggcgaga agctgcgaac ggaagagggc gatcggatcg tccttgcgca tgcgctcaat
      241 ttcttcgctg ttgcggtagc gctctggatc gcccatcgag tgcccgcgat atcggtaggt
      301 aacgatttcg agaaagtagg gggtgctgga ctcccgcaca gttgccaagg cgtcctgagc
      361 ggccgcccgg accgccagga cgtccatgcc gttcacgctg gaattcggca tggcatagcc
      421 ttcggccttc tgtctcatct ccaggacggc cgaggagcgc tcgaccgaag ttcccatccc
      481 atatttgtta ttctcgcaga tccagagcac cgggagtttc cacaccatcg agagattgag
      541 cgattcgtgg aagtagccaa tattggtggc cccgtcccca aacatgcaga tcgtcaccgc
      601 gtcatcgccg cgatactgat cggccagcgc catgcccacc gccaaaggca ggtggccacc
      661 gacgatggca tagccgcccc agaagttctt gtcgacgtcc gccaggtgca tcgagccgcc
      721 tttgcctttg gcagccccgg tggacttgcc gagcagctcg gccatcacga ccttggcctc
      781 aatcccacag gcgatggcta caccatggtc ccgataggcg gtgagcactc gatccttggg
      841 cagccggacc gagagtactc cgctcgagac ggcctcctgg ccaatgtaca ggtgcaggaa
      901 accgccgatc ttcccttgct gatagagccg ggccgcctct tcttccaaac ggcgaaccag
      961 caccatctca tggtagagac ggagcatttc tttctcgtcc agggctttga ttgcgacgtc
     1021 aatctgcgcc atggggcgcc tccatcacag cttcaacacc cggccatggg ctgggttgcg
     1081 gccggcaacg attagccccg gtcgcccggg gccagcgaga ttatatcaga ggggttattc
     1141 ctccgccttc aagggggacc caagagccct caagtcgaac tcgacgtcgc ccggatcggt
     1201 ccccgtaggc agcggtaggc ggatcacgaa ctcagtctgt tccccggagg ctagtggtcc
     1261 gggagtcgtg aaccaaccgg ccgagagcag ctcgccatcg gtggagcgta gctctgcaag
     1321 gactgcggcc gggtccacgg cctgcagagt ctcgttgcgc aaaatgcccc gtaggaacag
     1381 ggaggagccg accgagtgga aggcttcgac ctccgcctgc aggactattg gttgcacggc
     1441 cgggtcggca ccctcaatcc ggctttcgat ccgcaggctc gccggctccg acccagccag
     1501 ccggcggccg aggcctggga aatcctgggt tgcgaacgcg agccgttctc cggcttgaag
     1561 tggcactggg gcccgcagtt cggtcaggct caggattcga tctcccttcc acagcatgat
     1621 gagcacgctt gcctgagcgg gcgcgccggc cgcgtttctc aaacttccca ccacgaagac
     1681 gttgccttgc gaatcgactc gaactcgcgg ggcctcgtcc agtaccaggg agtcggcaac
     1741 tcgagccccg acttcgaccg catcatggta ggccttccag ttcagctggc ccgggttccc
     1801 tctcgccagt gccaagaagg gaacggattc ctcggcgccc agcacccgtg ggccatgctg
     1861 gagcacggcc acagcccgag gctgggaggc gccaccaaac ccgatcaatc cgagcgagac
     1921 gaacgattgg acctgggaac ttggattcgt gacggttccc agcagcgcga gctcgccggt
     1981 ctcggtgagg aactcttcga gcacctcgat ttccagtttc gaccgacgaa tgaagtgtcg
     2041 ctcgaaggaa tcgacccgag cctcagccgt caccgccggg cccaccccgg ggaactccgc
     2101 aatgaatgga ccggcctcgc ccggggcgaa gttggacata agagcgccgg tggtgagttc
     2161 agccagctgg ttgccgtggc tgtcggcgac ggtcacctgg atccggacgt ttgaaacccc
     2221 aacttcggct gggttgcgca acatgccgat tacctcccag tccccgggcg cttctcccgc
     2281 gagcaacacg atcgaattga actcaagcga agggagggcg gtggctgtgg ctgtggctga
     2341 aacgggggtt gtggccggcg gggattgaaa gtcggatggc tgaccattgc cgacctgagc
     2401 ggcgcaggcc gagactagga gcaaagcgaa caagcggatc gagtggatcc aaactgtcac
     2461 ggtggagagc tagggcaggg accggagggc ggctattggt taaaggtctc ttcgaagctt
     2521 agatcgaact ctaagggttc cgaagagccg agacctgctc cctctccgac ctggcacagt
     2581 gcactcgtgt cggagagcaa gcagtcggag agcaacccct gaccgacgct ttccagcaag
     2641 gagaccaatc cggcctgatc gctggccagc agcactaatg tggtgcgagc ttccgaccgg
     2701 gcgaggccga caaacccgat ccccgccgga ttgaacgagc cgaatccggg ggccgccaat
     2761 cggccgtccg atccctctgc cggaagcacc aggccgaggg gctcgatcaa ctcaccgagt
     2821 tcgggatccg gctcgaacag ccccaggacc agcagatcgc cttcggcggg aacctcctcc
     2881 ggaaaggaca aactaacccc ggcgggaagc agggccgtgg acatcccttg cagggcgacc
     2941 aaggtctcgg cctgcagggc gaaatccgca ctttgaacta ccgagactgg gcctgagaag
     3001 acgaagggca agttgtgcag atcgggccga cgatcgccgt cgagcagaaa ctccgccagg
     3061 ttttggatga ggcgggcatt gtccgcgacc ttatcgtaag ggggtgtcat gaaggtcagg
     3121 tcgcccaacg ccagggcctg ggagccggga tcgagggcgg cggccgccag accgcccccc
     3181 gtatcggtga gcgaggagag ggtcgccgaa tctccggaga gcagaagcgt tccagcttcg
     3241 gtctgcaccg atcgggcggc atacaacgcg acggtttgca agccggcggt gagcgggtgc
     3301 tcgccgaagt tgcgcagcaa gacgttgcgg aagttgccct cgttgtccat aaggttgtag
     3361 aggtaatcgt cggagaatgc caggttgaaa gattccagca actgattcgc tgtggaaacg
     3421 tcttcactgg cagacaggat gagaaagccg aagatatcga aaccggaacc ggtccgggtt
     3481 ggatcgagga ctaccagcag ccgcccgccg tgctcgacga agcgttcgac ggccgtcact
     3541 tcgctggggg agaagacgac tgaaggtgcg accaccacat acgagctggc gtacttcaat
     3601 cggccttcga gaccaagctc gtcaaatgcg aagctcgaat ccaatacttc gatccgtccc
     3661 ccctgggagc tcagggccgc agtcagcgaa cgaagttccg gcattgagaa ccggttcgag
     3721 tgggatcggt caaacagaac aactttcggc gggccggcaa gggggccctc aggcgccgct
     3781 gtcgcttccg gcaattcaat tgcggcctcc ccaaccggag gcaggggagg ggagaactcg
     3841 gagtaggccg gcagctggat cggagctggc gggcggtaga agccgcggtg gtaccacagc
     3901 gtccgcccga ccacgggaag gaagatcagg gccaagccga tccacactcg attgcgaatc
     3961 acggcaggct gcgctcgccg ggcggcacgt aaagtaggta aaacccgggg gctttcggat
     4021 acggagttag tgcgccctcc ggtgtctcga agaagaacgg aaccgagagc accgacggca
     4081 atccggctaa ttcgcgcaga tcggccaccc gatagttggc aacgtgactg agctcggccg
     4141 ctttctcgat cgcggccgaa attgatccga gctcatcgac caggccgaac cgggcggcct
     4201 cgctgccgag ccaaatctcg ccgcgcagga tccgttccgg accgattgcc agggcttcgc
     4261 cgcggccgag ctgcacggct gagtaaaatg cctgcttgat cgcttccatc tctcgcaagc
     4321 gggtgtcgcg ggccgagccg aacagcttgt atggaccggt agaggcgaga tcctcgaata
     4381 ccgagggagc ggtcggcagc tgggagatta cgccgacatt gccgacgagg gatgagggac
     4441 cggcaaagat gtagtcggtg ccgaccgaaa ggtagtaggc gccactggcc gccaggcctt
     4501 gggccatcgt gacgaccggc ttccgctcgc gcagcccggc aagttccagg tacaccgcct
     4561 cggtgtccac caccgttcca cccgggctgt ccaggactaa gaccaccgcc cgaacctcag
     4621 gatggtcacg ggcaaagtgg atctgggcga tcatgtcccg cgaggagaag gcgtggattg
     4681 cctcctgcag gtagatcaga cccacgatcg gtcgagggac gagcgctgcc agcccaaggc
     4741 cgacaagcag aggcaagaag atccaagcga gccagcgacg aaggccgcgc ttgtaagggt
     4801 ctggctcagg ggaggtcatc ggcaggactc cgaactcgac tggagcagcc ggatgctaca
     4861 ccgatttgtg gagcagggcg agcagccgag ccattggcgg agtgatctgg gagggcggag
     4921 aattcactgc ttgcccggcc ctagtaaagc ggcaagctcg ggtcaacctg gcgggcccag
     4981 gcgttgatcc cgcccttgag gttcttctgt ttgcggaagc cggctccggc cagcagttcg
     5041 accgcccggg cggatcgcga acctgacttg cagaacacga ccatctcttc agagctgtcc
     5101 agctcgtgca tccgtgcggc caatgatccg agcggaatga gcacggcacc ctcgatccgg
     5161 gagatttcga gctcgtgcgg ctcccgcaca tcgatcagcc ggaatcgctc accatttgcc
     5221 agacgagccg cgaggtcggt gggctcgatt tcaaactcgc tggccaacag actttcctcc
     5281 cgatcgtggc ccgggacgcc gcaaaaggcc tcgtagtcga tcagttcagt gacctcggga
     5341 ttctccgagc acactcgaca gccgggattc ttcttgagcc gcagctcttc gaagctcatg
     5401 ttgagcgcat cgaagagcaa cagcttgccg atcagcggtt ccccgattcc cagcagcagc
     5461 ttgagcgcct cggtcgcttg cagcgcgccg atcgtcccgg ggagcacccc aaaaaccccg
     5521 ccttctgcgc aagaaggaac cagacccgga ggcggtggct cggggaagag gcaccggtag
     5581 catggccctc gctcggccca gaacacgctc aattgcccct cgaagcggaa gatcgagccg
     5641 tagacgttgg gcttgccggt cagaactgcc agatcgttta ccaggtaccg ggtcggaaaa
     5701 ttgtcggtgc cgtcgatcag gatctcatac ggctcggcga tccgcatggc gttttcggag
     5761 gtgaagggct cgtcgtaaat gtccaccttg atctcggggt tgatgtccag gactctggcg
     5821 cgggctgaga tcaccttgcg ttcccccagg gtcgaggagc catgcaggat ttggcgctga
     5881 aggttggtga aatctacgac gtcgtagtca accaacccca gccgcccgac cccggcagcc
     5941 gccaggtaca tggcgaccgg cgagcccaac cccccggttc cgatgataag agccgaggcg
     6001 gccttcagcc ggcgctgtcc ctcaagcccg acttcgggca tgagcaggtg ccgcgagtag
     6061 cggaggatct cttcgtgggt gagttcgatt atgcctcgat tctaactctc aatcgctggc
     6121 cgccggtgtg gggctggccg gatgggctgc cccaagctca ccgatcgcgg ccagctgatc
     6181 caggaggtgg ggcgcccggg caaccccgtg gttgacgccc cgcggccggc cacccgagtc
     6241 gatgataaac gtggtcggca gcgataggac cccccaagca tcggtgagct cgggccgctc
     6301 cagcgcccgc accttgatga tctgcagacc ctcgccgaat tgggccgcca cttcctgcag
     6361 cgccggatcc tggaccgaaa ggcagggtgc acacccggga tcggtgaagt acaggatcgc
     6421 cggccgcccg ggccggtact cgtcaatccc caggccgtgg cgcgcccgcc tgcgcagcac
     6481 agcccagcca taggctcggt aggtgaggta cccgaggagg atgagcgcca gcgccagcgc
     6541 cagacggtcc aggctcatct caacccgggc gccgcccggg gaagaccccc ggtggggggg
     6601 ccttactgaa accggggacc ccgactcggc ttgaccagta gtagaccgcg caaccgacgc
     6661 aaaacccgcc aaatagattc agaccaacca acgcgatcac gatccaggtc agcacccagc
     6721 ccagggccgg cgatccagac ccgaatgcca tcgttgcgcc ggccaggaag gtcccaccga
     6781 cccccagcgc gaaacggtgc ggttccgggt tgtcctgaat cagctccggg ctaatccaat
     6841 ttgctttgcg cagggcgcga tagacgaagg tgaattccgg cttgccccga gccgcaccaa
     6901 tcagtatcag ggcggccagg atcgggacca gccagctttg atcggtaatg aagattgcga
     6961 gcaggaaccc cagtctcacg gccatgctgg tcttgagtga actgtggtcg acggctttca
     7021 aggtcgtcat tgttgtttaa cctcctgcca cgctcggaag aatcatgagc tggtcgctct
     7081 ccgctagcgg agtatggact ccgtccaggg ctcgcacgtc ctggtcgtta acgaatagat
     7141 tcacataggg ccgaagctgt ccctcgtcgc cgaacagatg gggggcgagt ccggggtacc
     7201 gctgggcgag ttcctgcagc gcgccgccca ccgtggcggc ctcggtcggg acttcaatct
     7261 gcccatcggt gtagggccgt agggcggtcg gaacccgaat atggctcatc tcgactcctg
     7321 ctcgatcgcg agctgctcct cgaccatctg cgcatgatct tcctgcagcc gccaggcgcg
     7381 ggtctgctcg gcgatgccct ggcggacggt cgtaatcaag tagatgtacc agggaacagc
     7441 ccaatccgcg tcgaatttcg atggcgtcgg cggatgatca gga
//
LOCUS       DATDPQ010000224         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2727708, whole genome shotgun sequence.
ACCESSION   DATDPQ010000224 DATDPQ010000000
VERSION     DATDPQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727708"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1231..1983)
                     /gene="istB"
                     /locus_tag="VI520_05110"
     CDS             complement(1231..1983)
                     /gene="istB"
                     /locus_tag="VI520_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HLF37295.1"
                     /translation="MLRHPTIETLQTLKLTGMATALAEQLEMPEVQSLSFEARLGLLV
                     DRELTTQENRRLARRLANARLRLPAALEDLDVRAPRGLDTGLIASLATGQWIRTHDNV
                     LISGPTGAGKTYLACALGQMACRLGFGAAYHRLSRLLQDLALARGDGRYRKLLRSVAK
                     ADLLLLDDWGLAPLTEEARRDLLEVLDDRHGRRSTVVASQLPVEHWHTAIGEPTLADA
                     ILDRLVHNAYKITLKGESMRKRLAKKPEVGHD"
     gene            complement(2000..3550)
                     /gene="istA"
                     /locus_tag="VI520_05115"
     CDS             complement(2000..3550)
                     /gene="istA"
                     /locus_tag="VI520_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HLF37296.1"
                     /translation="MPAERVTMRKIKDIVRLNETCQLSHRQIAKSCGVARSTVAEILR
                     RATTAGLSWPLPVDVDDATLEARLYPETPPITGTSRPVPEWATVHQELTRKGVTLALL
                     WQEYQAAHPDGYQYSRFCDRYRVWLKTLDRCLRQEHRAGEKLFVDYAGQTMPVRTRPT
                     GALREAQIFVAVLGASNYTYAEATWTQALPDWTASHVRAFAFFGGVPQIVVPDNLKSG
                     VTKTCRYEPELNPTYQALAEHYGVAVIPARVRKPRDKAKVEVGVQIVERWILACLRHQ
                     PFFSLSDLNAAIATCLTRLNARPFKKLPGCRRSVFDTVDRLALQPLPAEPYVYAEWRQ
                     ARVNIDGHLEVEGHYYSVPSPLIRTQLEVRLTATTLECFHHGQRVASHVRSAEKGHHT
                     TVVAHLPLAYQRYRDWPPSRLIHWAETVGPATAAVVTTILNGRPHPEQGYRSCLGVLR
                     LSREYGDPRLEAACQRAQTLGAFAYKSVQSILKTGLDQQPLTEPGSPRPLPVDHAHLR
                     GTTYYHEP"
BASE COUNT          749 a         1157 c         1285 g          755 t
ORIGIN      
        1 cgcaactcgg ctgaatcgcc taacctggcc attccattgg tgattctgtt ggttctggtg
       61 agggtgaaga agggtgagaa aatgggtctg tcgctatttg taaaccgcag gttggaggtt
      121 caattcctct cgccagctcc aacttttcag cgttattaga ggtcagaaaa gataaaaggt
      181 aacgaaaaag gtaacatgat ttagaagaag atgccctccc tgccgaatcc tctaatcgcc
      241 gctgctgctg acatcctctc atcgcactat tacagccacg ggcaaatcaa taatctcttt
      301 acccaatgcc atgcttcagg cccacctccg gaaggaaatc tggcccgcaa aataaccctg
      361 tggttgacgg aatcaaccga gcctttcgcc ctcctcggtt gcgttctcga aaacttcatg
      421 gaacaggtaa cggaatcacc cgattggctg gatcgccgtg caaaaatcaa tcgagccctt
      481 gccactcatg gactatcgta tgggcaaggg gggcacatta ttggcggcgc taccggagcg
      541 ccaagcagaa gcttggactc cattatccgt gagagagacc tcccggctgt tctagctgaa
      601 tttgatcggg cgctctccac ggtggagcga gatccaccgg cggccgtaac ggcagccggt
      661 gcaatcgttg aatccctctg taagggttac atcgcggaga atggtctcac cctgccatcg
      721 gatcaatcta ttcgcccact gtggaagatg gttcaagagc acttagggct cggtcctcga
      781 tccattgtga ccgatgatat acggcagatc ctaggtggct tggcgacggt gctaaatggc
      841 gtcggagatt tacggacgca tgcggggagt gcgcacggaa gaggtcacga agcattcaac
      901 gtcgagcccc gtcacgcacg gcttgccgtt catgcggctc acacgctggt tcacttcgtt
      961 ctggaaacgt gggaaaggac ctcggctcca tgagcatcaa agatgctcgc gaaaaaattg
     1021 ctcgggtgct cctagagaaa cagcttgagg agcgggacaa attagaaggc ccatatgaaa
     1081 gggcactcgg taagctggcg atacgttttt ctttactgca ctttacgctt gagatgttta
     1141 gctggtgcgt attccgacga agtcggccgc cgattccgag cgaatgcggc cacccctcgg
     1201 agcggagcga cgcaagctct ccaatgttac tcagtcgtgg ccgacttccg gtttcttcgc
     1261 cagccgtttg cgcatcgatt ctcccttcaa ggtgatctta taggcattgt gcacgagccg
     1321 gtcaaggatc gcatcggcga gcgtcggctc cccgatggcg gtatgccagt gctcgaccgg
     1381 gagctgactg gcgaccacgg tggaccgccg gccatggcga tcatccaaca cttcgagcag
     1441 atcgcggcgg gcctcctccg tcaggggagc caggccccag tcatccaaga ggaggagatc
     1501 cgccttcgcc acgctccgca agagtttgcg gtagcgccca tcgcctcggg cgagcgcgag
     1561 gtcctggagg agccgggaca gtcggtggta ggcagccccg aacccgagtc ggcaggccat
     1621 ctggcccagc gcacaggcca gataggtttt gccggcgcct gtggggccgc tgatcaacac
     1681 attgtcatgg gtgcggatcc attggcccgt ggccaacgag gcgatcagcc cggtgtccag
     1741 cccgcggggg gcgcggacat ccagatcttc cagggctgcg gggaggcgga gccgcgcgtt
     1801 cgccagccgc cgcgccagcc gccggttctc ctgggtggtg agttcgcgat ccaccaagag
     1861 tccgagccgt gcctcgaagc tgagggactg gacctcgggc atctccagtt gctcggccaa
     1921 ggcggtggcc atgccggtga gtttcagggt ctgtaacgtc tcgatcgtgg gatggcgtaa
     1981 catcgtgagg ctcctgggtt tagggttcgt ggtaataggt ggtcccgcgg agatgcgcat
     2041 ggtccaccgg gagcgggcgc ggggagcccg gctccgtgag gggctgctgg tcgaggccag
     2101 tcttgagaat cgactgcaca cttttgtagg cgaaggcccc cagggtctgg gcccgctgac
     2161 aggcggcttc gagtcgcggg tcaccatact cccggctcag gcgcagcaca cccaaacagg
     2221 accggtagcc ttgttcggga tgcgggcggc cgttcaggat ggtggtcacg acggccgccg
     2281 tcgcggggcc cacggtctcg gcccagtgga tcagccggga gggcggccaa tcccgatagc
     2341 gctggtaggc caggggcagg tgcgcgacca cggtggtgtg gtgccccttc tcagcactgc
     2401 ggacatggct cgccacccgc tggccgtggt ggaagcactc gagggttgtg gcggtgaggc
     2461 gcacctcgag ctgggtgcgg atcaagggcg acggcacgct gtagtaatgc ccctccacct
     2521 cgagatgccc gtcaatgttg actcgggcct gccgccactc ggcatagaca tagggttcgg
     2581 cggggagcgg ctgcagcgcg agccggtcca cggtgtcgaa gaccgagcga cggcagccgg
     2641 ggagcttctt gaaggggcgg gcgttcagcc gcgtcaggca ggtggcgatc gcggcattga
     2701 ggtcggagag actgaagaag ggctgatggc gcaggcaggc cagaatccac cgctcgacga
     2761 tttgcacgcc gacctcgacc ttggccttgt cgcggggttt ccggactcgg gccggaatga
     2821 cggccacgcc atagtgctcg gccagcgcct gatacgtcgg gttgagttcc ggctcatacc
     2881 ggcaggtctt ggtgaccccg cttttgagat tgtcggggac cacgatctgg ggcacgcccc
     2941 cgaagaaggc gaaggcccgg acatgcgagg ccgtccagtc cggcagcgct tgcgtccagg
     3001 tcgcctcggc ataggtatag ttggaggccc ccagcacggc gacgaagatc tgcgcctcgc
     3061 ggagcgcccc ggtgggccgg gtgcgcaccg gcatggtctg ccccgcgtaa tcgacgaaga
     3121 gcttctcgcc cgcccgatgc tcttggcgca gacaccgatc cagggtcttg agccagacgc
     3181 gataccggtc gcagaagcgg ctgtactggt agccgtcggg atgcgcggcc tgatattcct
     3241 gccacaacag ggcgagggtg acccccttgc gggttaactc ctgatggacc gtcgcccact
     3301 ctggcacggg ccggctggtc cccgtgatcg gcggagtctc cggatacaac cgggcttcca
     3361 gggtcgcgtc atccacatcc acgggcaagg gccaggacaa tcccgcagtc gtcgcccgcc
     3421 gcaggatctc ggccacggta ctgcgcgcca ccccacaact cttggcgatc tgccggtggc
     3481 tgagctggca cgtctcgttg agtcgcacta tgtctttgat ctttcgcatc gtcactcgct
     3541 ccgctggcat cggctgctcc tcccattaag atggaagggg cacgataccg aaggttgcgg
     3601 aggacttgcg ccgcgattcg cgcggaactg tcagcggcgg atcgccgccc ctcattccga
     3661 gggaggtcgg ccaccgattc cgaggaagct cagaatagtg gccggattcc catcggaatc
     3721 agtggccggg ttccgttcgg aatgggtggc cgacttgcgt cggaatcagt ggccgggatg
     3781 ggtcggaata cgcagtttgg gaaagaagtg gctcggctga gaaagcgact ggctatatcc
     3841 caagaggaac tagcgtttcg agcggaagtt caccggacct acgttagcca acttgagaga
     3901 gggctgaagt caccaacgct gagtatgatt ctcaagctgt ctcgtg
//
LOCUS       DATDPQ010000225         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1680077, whole genome shotgun sequence.
ACCESSION   DATDPQ010000225 DATDPQ010000000
VERSION     DATDPQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680077"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VI520_05120"
     CDS             <1..314
                     /locus_tag="VI520_05120"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HLF37297.1"
                     /translation="AATPLTLDNQPPSIELLSPQPGLRIRLASQASLLLQAEAGDAVK
                     LEQVEFLVNGRPVATMANAPFLFSWVLPNRTGAFEIRGRATDAAGNQAESELVTIEII
                     P"
     gene            complement(325..>2340)
                     /locus_tag="VI520_05125"
     CDS             complement(325..>2340)
                     /locus_tag="VI520_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37298.1"
                     /translation="RYRIEGHLGKGGMGTVYLAYDQTLELRVALKENLNPHPESEQQF
                     RREAKLLASLRHPNLPRVTDHFILRDVQYLVMDYIEGEDLQTRLMRQLPDTAEAIRWA
                     DDVCEALNFLHTRQPPIIHRDLKPGNLKLQPDGRVILVDFGIAKAFTQDQTATGARGM
                     TPGFSPPEQFGGSGTDPRSDQYSLAATVYYLLTGKQPANSIDRTLKQGDLQPPREINP
                     KIPTHVEAALLHAMALRKDDRFQDVATFKAALHGGVDFETVQAGGGLSETIRAEALPR
                     PARKRLPVALVAGSGVLALILIAGVGLARGGQQTQPSPTVLGGGLATATLTPAPTETA
                     LSTPTSSTTPSPAPSLTVTSVPTLTAVPVVVGGSAKLAFVSDRAENVLQIWTMNPDGS
                     EPRQLTFDPGDKTYPTWSPNGSRLLYVAPGGDDAAGADLGLDIWIINADGTGIERVVS
                     WPGHDTEPAWSPDGKLIAFTSTHANNTRQVFLLDASCLDQSGGCVDQRPRNLSCHPDF
                     CAVEFSPAFAPTAVKLPPFLTSAFTLAVLESINEAPAEIYFRPPEVEVPIDFDRRDQV
                     RGLEDLAWSPDGDYFAFTWFYQRGANEIYVAAVAKRGASPERLTFTNGNREPAFTPDG
                     QWIAFSTTRDGKPEIYLMTLGGANQVNLSLSAASRDQQPDWQPLVAP"
BASE COUNT          424 a          686 c          801 g          429 t
ORIGIN      
        1 ccgccgcgac cccgctgacc ctcgacaacc aacctccctc gatcgagctg ttgtcccccc
       61 aacccggact ccgcattcgc ctggccagcc aggcgagcct gctgctgcag gctgaagcgg
      121 gagacgcggt gaagctggag caggtcgagt ttctggtgaa cggacgcccg gtggcgacga
      181 tggccaacgc gccgttcctg ttcagctggg tactgcccaa tcgcacggga gccttcgaga
      241 tccggggacg ggcgaccgat gcggccggca atcaggccga aagcgaactg gtaacgattg
      301 agatcattcc gtgagagaga gaggtcaggg agcaaccagc ggctgccaat cgggctgctg
      361 atcccgggag gccgcactga gcgacaggtt gacctggttg gcaccgccga gggtcatgag
      421 atagatctcg ggcttcccat cccgagtggt gctgaaggcg atccactgac cgtcgggtgt
      481 gaaagccggt tcccggttgc cgttggtgaa ggttagtcgc tcaggcgaag cgccccgctt
      541 agcaaccgcg gccacgtaaa tctcgtttgc tccgcgctga tagaaccagg tgaaggcaaa
      601 atagtcgccg tccggcgacc aggccagatc ctccaagccg cgcacctggt cccggcgatc
      661 aaagtcaatc ggaacctcaa cctccggcgg ccgaaagtaa atttcggccg gggcttcgtt
      721 gatggattcg agcaccgcca gggtgaaggc cgaggtcagg aagggaggca gcttaaccgc
      781 ggtcggcgcg aaggccggtg agaattcgac cgcgcagaaa tcgggatggc aagagagatt
      841 gcgcggacgc tgatcgacgc accccccgga ttgatccaga cagctggcgt ccagcagaaa
      901 tacttggcgg gtgttgttgg cgtgggtgct ggtgaatgcg atcagcttgc cgtccggcga
      961 ccaggcgggc tcggtgtcgt gcccgggcca gctaaccact ctttcaatgc cggtgccgtc
     1021 ggcgttgata atccagatat cgagtccgag atcggcgccc gccgcgtcgt cgccaccggg
     1081 ggcgacatac agcaggcgtg aaccgttggg cgaccaggtt ggataagtct tgtcgcccgg
     1141 atcgaaggtc aactggcgcg gctcgctgcc atccggattc atggtccaga tctgcaggac
     1201 gttttcggcc cggtcgctga cgaaggcaag cttggcggac cccccgacca cgaccgggac
     1261 tgccgtgaga gttggcaccg acgttacggt aagacttggc gccggacttg gggtggtaga
     1321 ggaagtgggg gttgagaggg cagtctcggt cggcgctggg gtgagggtgg cggtggccag
     1381 ccctccgccg aggactgtcg gtgacggctg agtctgttgt ccgccgcggg ccaatcccac
     1441 gccggcaatc aggatcaggg ccaacacccc gctgcccgcc accaaggcga ccggtaggcg
     1501 cttgcgggcc ggccgcggca gagcttcggc tcggatcgtc tcggagagtc ccccaccggc
     1561 ttggaccgtt tcgaaatcga ccccaccgtg cagcgccgcc ttaaaggttg ctacatcctg
     1621 aaaacgatcg tccttgcgca gcgccatggc gtgcagcagc gctgcttcca catgggtagg
     1681 gatcttgggg ttgatttcac ggggtggttg taaatcccct tgtttgaggg tgcgatcgat
     1741 gctgttggcc ggctgcttgc cggtcagcag gtaatacacg gtagccgcta gggagtactg
     1801 atcgctccgg ggatccgtgc cggagccgcc aaactgctcg ggtggggaga acccgggggt
     1861 catcccgcgg gcgccggtgg cagtctgatc ttgggtgaag gctttggcga tgccgaagtc
     1921 gaccaagatc accctgccat cgggttgcag tttcagattg ccgggtttga ggtcgcgatg
     1981 gatgatgggc ggttggcgcg tgtgcaggaa gttcaacgcc tcgcaaacgt cgtctgccca
     2041 gcgaatcgct tcggccgtgt cgggaagctg gcgcatgagg cgggtctgca ggtcctcgcc
     2101 ctcgatatag tccatcacca gatactggac atctctcagg atgaagtgat cggtcacccg
     2161 gggcaggttg ggatggcgaa ggctggccag cagcttggcc tctcgccgaa actgctgttc
     2221 ggattcaggg tgggggttga ggttctcctt gagcgcaacc ctcagctcga gagtctgatc
     2281 ataggcgagg tagaccgtgc ccattccgcc cttgccgaga tgcccctcaa tccgatagcg
//
LOCUS       DATDPQ010000226         2607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_617816, whole genome shotgun sequence.
ACCESSION   DATDPQ010000226 DATDPQ010000000
VERSION     DATDPQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617816"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VI520_05130"
     CDS             complement(<1..927)
                     /locus_tag="VI520_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF37299.1"
                     /translation="MLRVVASLFRFNVILLAGLALAPAACQAGSAPSDEPLQWTDGLG
                     RQVALSAPAQRIVSLAPSNTEILFAIGAEAQVVGRDEPSDFPAEAAEVSSIGSTYGQL
                     NTEAIVALQPDLVLAASITAPEQVQALEDLGLTTYVLADPSDFEGLYQNLTIVGELTG
                     QQAGAAELLAELEARVTAVAERMQGADRPRVFYEADASDPTAPWTTGSGTFQSVLIDL
                     AGGTNVAAEITGWGQFSLEQLVAVDPQVIVFGSGSFVPTTVDSLAARPGWGTITAITD
                     GRVFAIDTNWVDRPGPRLVEALEAMARVIHPEL"
     regulatory      complement(1067..1265)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1442..>2607
                     /locus_tag="VI520_05135"
     CDS             1442..>2607
                     /locus_tag="VI520_05135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HLF37300.1"
                     /translation="MASKNLRIVPLGGLGEIGKNMMSIEYGENLIVVDAGIMFPENDM
                     LGIDYIIPDFGYLKERKDKVRGIVITHGHEDHTGAIRHLLDDIPAPIYATKLTRGLLE
                     VKLSRGGMLKQATLNTVSAGETLEIGPFKLEFFHVCHSIPDCVGLGITTPAGLVVHTG
                     DYKFDHTPVDGWPTDFGKLGELGGRGVLALLADSTNADEPGWTPSEALIDPAFDAVFR
                     EAKGRILIGTFASLISRMQQAAQAADRHGRKMAFVGTSMQENAKMARKLGYLDVPDEL
                     IVTPEQAFNMNPAEVLLMCTGTQGEPSSILGRLSTSTNRQFDLLPGDTVVLSSHPIPG
                     NEEMVHRTINRLFHRGAEVIYDPIAPVHVSGHASQEEMKVMLQLVRPKFFIPIHG"
BASE COUNT          514 a          825 c          784 g          484 t
ORIGIN      
        1 gagctcggga tggatgacgc gggccattgc ttcgagcgct tccaccaggc gcggtccggg
       61 ccgatctacc cagttggtat caatcgcaaa cacccgtcca tcggtgatcg cggtgatggt
      121 tccccatcct gggcgggccg ccaacgagtc aaccgtcgtc ggcacgaacg atccggagcc
      181 aaacacgatg acctgcgggt cgaccgctac cagctgctcg agactaaact ggccccaacc
      241 cgtgatctct gccgccacat tggtgcctcc cgccaggtca atgagcaccg attgaaaggt
      301 cccactgccg gtcgtccagg gagcggtcgg atcgctcgcg tctgcctcgt agaacactcg
      361 aggtcggtcc gccccctgca tcctctccgc cactgcggtt actcgggctt cgagttcagc
      421 cagcagctcg gccgccccgg cttgctggcc ggtcagttct ccaacgatcg tcagattctg
      481 atagagccct tcaaagtcag atggatccgc aagcacgtag gtcgtcaggc ccaggtcctc
      541 caaagcctgc acctgttccg gcgcggtgat cgaggctgcc aacaccaggt caggctgcag
      601 ggcgacgatc gcttccgtgt taagctgccc ataggtgctc ccgatgctgc tcacctcagc
      661 cgcctccgcc ggaaaatccg acggctcatc tctgcccacg acctgtgctt ctgccccaat
      721 cgcgaaaagg atctcggtat tggagggggc cagtgagacg attctttggg ccggggctga
      781 cagcgcgacc tggcgcccca ggccgtcggt ccattggagc ggctcgtcgc tgggggccga
      841 tccggcctgg caggccgcgg gtgccaaggc caagccggcc agcagaatta cattgaatct
      901 gaatagtgac gccacaacac gtaacatgag tgtcctccaa ggtctggacc ggtccaaccg
      961 gtcaacgatt gagaaccgtg gggaaagcaa aatggccttc cctcacggca gtgcgggcaa
     1021 ggccatcgtc acgaggacca tgcgccacct cctctccgcg aaggggtaac cagcgcgggg
     1081 cagaggcggg tatcctgact cggagaagcg gctactttgc ttctccctac agttgcggga
     1141 cagtgccgga cttccaatgg gggcggacgg cctggttagg caatctggat ccccgagcgg
     1201 tcaccggctt cccctggttg gcctccgcat ccgggcggta ggccacctcg gccccaggtg
     1261 gtaaattgtc tgcatactat attcgggcaa gcaatgtggg tcaagggatg atagaattga
     1321 tgggcccctt cgtattgatc cttcgaacga atgcggtttt cccctcggcg gcgcgggtcc
     1381 ggaactggca gagaacccga tccgatctcg agcaaactca tagacatagg taaaacacac
     1441 aatggctagc aagaatttac gaattgtccc cctcggtgga ctgggggaga tcggcaagaa
     1501 catgatgtcc atcgaatacg gcgagaactt gatcgtcgtc gatgccggga tcatgtttcc
     1561 cgagaacgac atgctgggga tcgattacat catccccgac ttcggttact tgaaagaacg
     1621 aaaagacaag gtgcggggca tcgtgatcac tcacggccac gaggatcaca ccggcgcgat
     1681 ccggcacctg ctcgatgaca tcccagcccc gatctacgcc accaagctga cccgcgggct
     1741 gctggaggtc aaactgagcc ggggcgggat gctcaagcag gccacgctga acacggtgag
     1801 cgccggcgag acgctggaga ttggcccctt taagctggag ttcttccacg tctgtcactc
     1861 gatcccggat tgtgttggcc tcggaatcac cactcccgcg ggattggtcg ttcacaccgg
     1921 cgattacaag tttgaccaca cgccggtcga cggctggccg accgatttcg gcaaactggg
     1981 cgagctcggc gggcggggag tgctggcgtt attggccgat tcgacgaatg ccgacgagcc
     2041 cggctggacc ccctccgagg cactgatcga cccggctttc gacgccgtgt ttcgggaggc
     2101 gaaaggccgt atcctcattg gcaccttcgc ctcgctgata tcccgcatgc agcaggccgc
     2161 ccaggccgcg gaccggcacg gccgcaagat ggccttcgtc ggaaccagca tgcaggagaa
     2221 cgccaagatg gcccgcaaat tgggctacct ggatgttccg gacgagttga tcgtgacccc
     2281 cgagcaggca ttcaatatga atcctgccga agtgctgctg atgtgcaccg gcacccaggg
     2341 ggagccttcc tcgatcctgg gtcggctctc caccagcacc aaccgccagt ttgacctgct
     2401 gcccggcgat accgtcgtac tctcctccca cccgatccca ggcaacgagg agatggtgca
     2461 tcggacgatc aatcgcctct ttcatcgggg ggccgaagtg atttacgatc cgatcgcgcc
     2521 ggtccacgtc tccgggcacg ccagtcagga agagatgaag gtcatgctgc agctggtgcg
     2581 gcccaagttc ttcatcccga ttcacgg
//
LOCUS       DATDPQ010000227         3552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1931593, whole genome shotgun sequence.
ACCESSION   DATDPQ010000227 DATDPQ010000000
VERSION     DATDPQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931593"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VI520_05140"
     CDS             <1..435
                     /locus_tag="VI520_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLF37301.1"
                     /translation="VLILGTMFIHSFPEGLAIGVAYGSGIASLGLLMTVAIAVHNIPE
                     GVAVSLPLRAEGVSGWKCVGWAIFSSLPQPIAAIPAFLAVQAFRPVLPFALGLAAGAM
                     FFLVLSEMIPESRADEGQRLSSALATMVGFLALMVLQNVMVF"
     gene            601..1629
                     /locus_tag="VI520_05145"
     CDS             601..1629
                     /locus_tag="VI520_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HLF37302.1"
                     /translation="MESFQILLALLAAGCALAIAAALFWPERGWLGRWRSRRRQMQLE
                     DCLKYLHTAELESVPATLPGLSAARRISLSLAAEILLNLERLGFVERKASAIQLTPAG
                     RRRALLVLRAHRLWERYLADETGYRELEWHKQAEKQEHRLTPPELEALAARLQNPTYD
                     PHGDPIPTRAGETPPQLGMPLATAPLDTPLRIAHIEDEPEPTYAELLAVGLSPGMELQ
                     VVERSAAQMRIRVGQDEHILRPPVAANLLVEPQPGTGVQAAQARLSDLRVGQRGAVVD
                     IAQGCRRIERRRLQDLGVLPGTVIEAEFASPSSDPVAYRIRGALIALRREQAAHILVD
                     RLEDTRES"
     gene            1619..>3552
                     /gene="feoB"
                     /locus_tag="VI520_05150"
     CDS             1619..>3552
                     /gene="feoB"
                     /locus_tag="VI520_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HLF37303.1"
                     /translation="MSPSHRGCEGCPVHASANLAKLGVDMNRWDFLVALGGNPNTGKS
                     TVFNALTGLRQHTGNWPGKTVAKAEGGYEFAGRRYKLVDLPGTYSLLATTVDEQIARD
                     FILSGQPDVTVIVVDATRLERNLNLVLQVQEITDRVVVCLNLMDEARRHGLAIDDRQL
                     ARDLGVSVVPITARQGVGLDRLIATVAEVASGKLISRPRQIADPQVQEPGQSAERPDQ
                     RIDGNRHAQSVEASYAEAARLAGRAVRQAEARPRFDLDRAIDRVVTSRVWGFPIMLLL
                     LAGVFWVTITGANVPSRLLGTFLLETVHPWLREAAADLGVAGWLRGVLIDGMYLATAW
                     VISVMLPPMAIFFPLFTLLEDFGYLPRIAFNLDTLFKRAGAHGKQSLTMAMGFGCNAA
                     GVISARIIDSPRERLIAILTNNFALCNGRWPTQILIATLFLGALVPARLAGAVSALAV
                     VGVAVLGVGLTFAVSWLLSRTVLRGEVSAFSLELPPYRPPRVLKTLYRSVADRTVYVL
                     WRAIVFALPAGAVIWIVANLQLGGVSLAERLIEMLNPFGLVLGLNGVILLAFVAAIPA
                     NEIVIPTILMLTVLVSQLSGVGAGAGVMFQLESGAGTLAILRAGGWTLLTGVNVMLFS
                     LLHNPCSTTIATIYKETGSKR"
BASE COUNT          568 a         1094 c         1195 g          695 t
ORIGIN      
        1 gtgctgattc tggggacgat gtttattcat agctttcctg aggggttggc gatcggagtg
       61 gcctacggct cggggatagc ttcgctgggg ctgctgatga cggtggccat cgcggtgcac
      121 aacatcccgg agggggtggc agtatcgctg cccctgcggg cggagggcgt gagcggctgg
      181 aagtgcgtgg ggtgggcaat cttctccagc ctgccgcagc cgatcgcggc gataccggcc
      241 ttcctggcgg tgcaggcctt tcggcccgta cttcccttcg ctctcggcct ggcggccggg
      301 gcgatgttct tcttggtgtt gagtgagatg attccggaga gccgcgccga tgaaggccaa
      361 cggctttcga gcgccctggc caccatggtt ggattcttgg ccctcatggt cttacagaac
      421 gtgatggtgt tctaggcggt gcccgatcgg tcccctgggc ggctgcctgt gatttagatc
      481 cccctaaatt ccctattggg ttaggcgaaa cctgtgacat ttgtcactgt tgtttcgggc
      541 cgggcgcggg catcctcggg ttggcgcggg aagttgcgcc ggattgaact gacacacccg
      601 atggagtcct tccagatact tcttgcccta ttggcagccg gctgcgccct ggccatcgcc
      661 gcggcactgt tctggccgga gcgcggctgg ctcggccgct ggcgcagccg gcggcggcaa
      721 atgcagctcg aggactgcct gaagtacctg cacaccgccg agctggaaag tgtgccagct
      781 accctcccag gcttgtcggc ggccaggcgg atcagcttgt ccttggctgc cgagattcta
      841 ttgaatctgg agcggcttgg gttcgtggag agaaaggcga gtgcgatcca gctgaccccc
      901 gccggccggc gccgggcgtt actggtgctg cgggcccacc gtttgtggga acggtatctg
      961 gcggatgaga ccgggtaccg cgaactcgag tggcataagc aggccgagaa gcaggaacac
     1021 cggctgaccc cacccgaatt ggaggcgctg gcagcccgcc tgcagaatcc gacctacgat
     1081 ccgcatggcg atccgatccc gacccgcgcc ggcgagacac ccccacaatt ggggatgccg
     1141 ttggcgactg cgccgctgga tacacccttg cggattgccc acatcgagga cgaaccagag
     1201 ccaacttatg ccgaattgct cgcggtaggg ctgtctccgg ggatggaact ccaggtggtg
     1261 gagcggtcgg cggcccaaat gcggatccgg gttggccaag acgagcacat actgcgcccc
     1321 ccggtggccg ctaatctgct cgtagagcct caaccgggaa ccggggtgca ggccgctcaa
     1381 gcccggttga gcgacctacg ggtcggccag cgcggagcag ttgtggatat tgcccaagga
     1441 tgtcgtcgga tagaacgccg ccgcttgcag gacctggggg ttctgcctgg gactgtgatc
     1501 gaagccgagt tcgccagccc gagctcggac ccggtggcct atcgtatccg aggcgcgctg
     1561 attgccctgc ggagggaaca ggccgcgcac atcctcgtcg atcggctgga ggacacccgt
     1621 gagtcctagc caccgcggct gtgagggctg tccagtccac gcgtcggcca acctggcgaa
     1681 gctgggggtc gacatgaacc ggtgggattt cttggtggcg ctggggggca accctaacac
     1741 gggcaagagc acggtcttca acgcgctgac cggcctgcga caacacaccg gcaactggcc
     1801 aggcaagacg gtcgccaaag ccgaaggcgg atatgaattc gcaggccgcc gctacaagct
     1861 ggttgacctg ccgggcacct attcgttgct ggctacgacc gtagatgagc agatcgcccg
     1921 cgacttcatt ctgtccgggc agcccgacgt gacggtcatt gtcgtcgacg caactcgctt
     1981 ggaacgcaac ctcaacctgg tcctacaggt gcaagagatt accgaccggg tggtggtctg
     2041 cctcaatttg atggacgaag ctcggcgcca tggcctggcg atcgatgacc ggcaactggc
     2101 ccgcgacttg ggggtgtcgg tcgttccaat cacggctcgc cagggagtag gactggatcg
     2161 gttgattgcg accgtcgccg aggtcgccag cggaaagttg atcagtcgac cgcggcaaat
     2221 cgccgaccca caggtccagg agccggggca gtcggccgag cggcccgacc agagaatcga
     2281 cggcaaccgg catgcgcaga gtgtcgaagc tagttacgcc gaggcggccc gccttgctgg
     2341 aagggccgtc cgccaggcgg aagctcggcc gcggttcgat ctcgaccgag ccatcgatcg
     2401 agtcgtgacc agtcgagtgt ggggcttccc catcatgctg ctcctgcttg ccggagtatt
     2461 ttgggtcacc atcaccgggg caaacgtacc gtctcggttg ctcggcacgt tcttgctgga
     2521 gaccgtccat ccctggctgc gggaagcagc cgcggatctc ggggtggccg gctggctgcg
     2581 gggagtgctg atcgacggga tgtacctggc cacggcttgg gtcatcagcg tcatgctgcc
     2641 gcctatggcg atcttcttcc cgctcttcac tttgctcgag gatttcggct atctgccgcg
     2701 gatcgccttc aacctggaca cgctctttaa gcgtgcgggg gcccatggca aacagtcgtt
     2761 gaccatggcc atgggtttcg gctgcaacgc cgccggtgtg atttcggccc ggatcatcga
     2821 cagcccccgc gagcgcctga tcgcgatcct caccaacaac ttcgccctgt gcaatggccg
     2881 ctggcctact cagatcctga ttgccacgct cttcctcggg gcactggtgc cggcccggct
     2941 ggccggcgcg gtctcggcac tggccgtggt cggtgtggcg gtcctgggag tcggcctcac
     3001 tttcgccgtc tcgtggctgc tctcccgcac ggtgctgcgc ggtgaggtct cggccttcag
     3061 cctggagctg ccgccctacc gaccgccgcg ggttctgaaa actctgtatc gctccgtcgc
     3121 cgaccgaacc gtctacgtgc tttggcgggc gattgtcttt gctctgccgg caggtgcggt
     3181 gatctggatc gtcgccaacc tgcagcttgg cggagtcagc cttgccgagc ggctgatcga
     3241 gatgctgaat cccttcggcc tggtgttagg gctgaatggc gtgatcctgc tggcctttgt
     3301 cgcggcgatt ccggccaacg aaatcgtgat tccgacgatc ctcatgctca cggtcttggt
     3361 cagtcagctg agcggggtcg gggcgggcgc gggcgtgatg ttccaattgg agtccggcgc
     3421 cgggacgctc gccattctgc gggccggcgg ttggacgctg ctcacgggcg tcaacgtgat
     3481 gctgttcagc ctgctccaca acccctgcag cacaacgatc gctaccatct ataaggagac
     3541 gggcagcaag cg
//
LOCUS       DATDPQ010000228         3889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_887918, whole genome shotgun sequence.
ACCESSION   DATDPQ010000228 DATDPQ010000000
VERSION     DATDPQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887918"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2434
                     /locus_tag="VI520_05155"
     CDS             <1..2434
                     /locus_tag="VI520_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37304.1"
                     /translation="ASLASLLGNHGSRPTALRLLRTGLRIRPHDVRLRRQLAELKEAA
                     GDQEGALQAWLSSAEVEPHEPGPLIRGAAIAGRMGELERSVELLEQAVGLAPRDASAR
                     RELARAYLRLGRLESGFAAYEAAMKELPGDGLLVIEVTEAALQAGAIERIDPLIERAR
                     QVVVEPARIHAALGEAHLARNRWDEAGQALGEAIAAGDTSPRVLAMTAIVASRSDLGQ
                     AKEALVRASQKACKSAHDAIWMSRAQLRMLNWDAALAALEPWISDPYGAREKAEVLLR
                     IASAHSLFNAVEARASAPGMDEHGLISAAESLINELTRQGLAAEALRPLRSLLNATLM
                     PRGGDQEAEQAEPEPTGQFGEAMAIGLLRAGQGARALEALAHIARVNPDSEWTPLLEG
                     LSHEFAGSPPAARAAYRRCAEDPILAPLGNFLLGRSYGRAHRPELATSHLNAAVAAWP
                     AEHAWQHALGIHYAELNELDASLAHLQQAVVGDSSNPAYRLDLARVLRRSGQLGLAEE
                     AFSLVLQSRADSSEALREAAETALELGKVEKAADWFERARALDPTDHRNLLGAARAAS
                     ERGNQKIASELLAAAARISPGSAEVLMAEGQIRARGGELEPAVQAFEAALEAGAELTA
                     VRRAQSKLLLERGQAGRAVTALEQALEADPEDHALWHQLALAREANAEWAAADLAVSE
                     SIQSFPMNTEYRLSSGRIARRAGQLDRAIDELLRAGELAPNDPRIYVETGKVYEDRRE
                     YSRALDSYRKAIALDPGALEAFYRAGVLLRSLKSYKNAGELLKRAAELAPVDQDVLHQ
                     LAAVRALELVHG"
     gene            2427..>3889
                     /locus_tag="VI520_05160"
     CDS             2427..>3889
                     /locus_tag="VI520_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37305.1"
                     /translation="MASLTDRETFISLLHAAALARRFDFARNVAADWLTAWPGDAEVR
                     LQLARSELDLGLVKPATERLLEIVAADPECIEGYSLLVRAYRQAADTLRPALYEACHQ
                     ALTDGSPDRASAPAWALPLSRAVRALSDGDPTEAVRASQEALTSDPSLPLPSIVAVKA
                     QLAASDFGAACAIAEIAHDRWPDCLGFQLILAIAGLSTDRQAASVAALQRLAAADPVG
                     IVADSLLGAGHPYRRLWPAKMDAILSRPVPAPVASVMGGTYLAEPVPASDRKRLRSRQ
                     VDTRGHRKMPSPEPGETFQGPDSGDEVASSQPSETLIAVRSEFERLAQRLNARQPSAG
                     QDQRLPAYIVLSSHTRLVQELGEPAFARVDGAIATLVDTVRRKRNWAAYRFYVDDPKY
                     LATFGLHPCDPGNAWQIKLRLADLDQALKSRGEMVGAVLIVGGHSIVPFHMLPNPTDD
                     DDDLVPSDNPYATSDENYFAPEWPVGRLPSDTEVDLLV"
BASE COUNT          671 a         1257 c         1297 g          664 t
ORIGIN      
        1 ggccagcttg gcctctttgc ttggcaatca cggctctcga ccgaccgctt tgcgactact
       61 gcgcactggg ctacggatcc gcccgcacga cgtccggctg cggcgacagc tggccgagct
      121 caaagaagcg gccggtgatc aagaaggggc gctccaggcc tggctgagct cggcagaggt
      181 cgagccccac gaaccaggtc ccctgatccg aggggcggct atcgccggga ggatgggaga
      241 gctggagcgg tcagtagagc tgctcgagca ggccgtcggc ctggcgcctc gcgatgcaag
      301 tgcccggcgc gaattggcgc gggcctattt gcggcttggc cggctcgaat ctgggtttgc
      361 cgcctacgag gctgccatga aagagctgcc cggcgacggg ttattggtga ttgaggtcac
      421 cgaagccgca cttcaggccg gtgcaatcga aagaattgat ccgttgatcg agcgggctcg
      481 acaggttgtc gtcgagccgg cacgaattca cgcggcactt ggcgaagctc atcttgcccg
      541 aaaccgctgg gatgaggccg ggcaggcgct tggggaagcg atcgcggcgg gggatacaag
      601 cccacgggta ttggccatga cggccatcgt ggcgagtcgc tccgatcttg gccaggcgaa
      661 agaagccctt gtccgtgcct cccagaaggc ctgcaagtca gctcacgacg cgatctggat
      721 gagccgggcc cagcttcgaa tgctgaattg ggacgcagcg ctcgccgcgc tggagccgtg
      781 gatttcggat ccttacggcg cccgcgagaa ggctgaagtc ctgctgcgga tcgccagtgc
      841 tcactcgcta ttcaacgcag tcgaggcccg ggcgagcgcg cccgggatgg atgagcacgg
      901 gctgatctcg gccgcagagt cactcatcaa tgaacttacg cggcaaggat tggcagccga
      961 ggcgctgcgg ccgctgcggt ccctattgaa cgcaaccctc atgccgcgcg gcggcgatca
     1021 ggaggcggag caggcagagc cagagccgac cggccagttc ggcgaagcca tggccatagg
     1081 ccttctgagg gccgggcagg gtgcccgggc gctcgaggcg ctcgcccata ttgcgcgggt
     1141 caatcccgat tcggagtgga cccccctgct ggaggggctg agtcatgaat tcgccggcag
     1201 tcctcccgcc gcccgggctg cctaccgccg atgcgccgag gatccgattc tcgccccgct
     1261 cggcaacttc ctgctcggtc gttcgtatgg ccgggctcat cgaccggaac tggctaccag
     1321 ccaccttaac gcggccgtag ccgcttggcc ggccgaacat gcctggcaac acgcgctggg
     1381 gattcattac gccgagctga acgagttgga tgcttcactc gctcacctgc agcaggcagt
     1441 ggtcggcgac agctccaacc cggcctaccg gctcgatctg gcacgggtgc tgcggcggtc
     1501 cggtcagctc ggcctggctg aggaagcttt ctcgttagta ctccaatcgc gggctgattc
     1561 gagtgaggcc ttgcgggagg cggcggagac cgcgcttgag ctgggaaagg tggagaaggc
     1621 cgctgactgg ttcgagcggg cccgcgccct ggacccaacg gatcaccgaa atctgctcgg
     1681 agcggcccga gccgccagtg agcgcggcaa tcagaaaatc gccagcgaac tgctggccgc
     1741 cgcggctcgc atctcccccg gcagcgcaga agtcctgatg gccgaggggc agatccgggc
     1801 ccggggcggc gagcttgagc ctgcggtgca agccttcgag gccgccctcg aggccggcgc
     1861 tgagctcact gcggtccgcc gggcgcaaag taagttgctg ctggagcgcg gtcaggccgg
     1921 ccgggcggtc acggctctcg aacaggcgtt ggaagccgac cccgaggacc atgccctatg
     1981 gcaccaactc gctttggccc gggaggctaa tgctgagtgg gccgcggcgg accttgccgt
     2041 aagtgaatcc atccaatcct tcccgatgaa cacagagtat cgcctaagtt ccggtcgaat
     2101 tgcccggcga gctgggcagc tcgatcgcgc cattgacgaa cttctgcggg ccggcgagct
     2161 cgcgccaaac gacccgcgga tctacgtcga gaccgggaaa gtctacgaag accgcaggga
     2221 gtattcccga gctctggatt cataccgcaa agcaatcgcg ctcgaccccg gggcgctgga
     2281 agccttctac cgggcgggag tgctcttgcg aagcctgaag tcctacaaga atgccggcga
     2341 gttgttgaag cgcgccgcag agttggcacc ggtcgatcag gacgtcctac accaactggc
     2401 cgcggttcga gcgctcgagc tggttcatgg ctagcttaac cgatcgagag acgtttatca
     2461 gcctgctgca cgcggcagcc ctggctcgtc gattcgattt tgcccgcaac gttgccgccg
     2521 attggctgac ggcctggcca ggagacgccg aggtacgcct acagctcgct cggtcggagc
     2581 tcgacctggg gctggtcaaa cctgctaccg agcgtctctt agagattgtg gccgccgatc
     2641 cggagtgcat agaaggctac tcgctgctcg tccgcgccta ccggcaggcc gcagacacgc
     2701 tgcgaccggc tctgtatgaa gcttgccacc aggcgctcac cgacggctcc ccagatcgcg
     2761 ccagcgcccc cgcctgggcg ctccctctct cgcgggcggt ccgggccctc tcggatggcg
     2821 atccgaccga agcggtacgg gcttcccagg aagccctgac ctccgacccc agcctgcccc
     2881 tgccctcgat cgtcgcggtc aaagcccaat tggcagccag tgattttggt gccgcatgcg
     2941 cgatcgccga gattgcgcac gatcgatggc cggattgcct ggggtttcag ttgatcctgg
     3001 ccatcgcggg actgagcacc gatcggcagg ccgccagcgt cgccgccctc cagcggctgg
     3061 cagcggctga tccggtggga atcgtcgccg attctctgct gggggcgggc catccctatc
     3121 gccggctgtg gccagcgaag atggacgcca ttctcagccg acccgtacct gcaccggtgg
     3181 cgtcggtcat gggaggcacg tatctagcgg aacccgtccc cgcttccgac aggaagaggc
     3241 tgcgctcaag gcaggtagac actcgcgggc atcgcaagat gccgtcgcct gaaccggggg
     3301 aaacctttca agggccggac tctggggatg aggtcgccag ctcgcagcca tccgaaaccc
     3361 tgattgcggt caggagtgag ttcgaacggc tggcgcagcg attgaacgcc cggcagccga
     3421 gtgcgggtca ggatcaacgg ctccccgcct atatcgttct ctcgagccac acccggcttg
     3481 tccaggaact gggggagccc gcctttgccc gggtcgacgg tgcgatcgca accctcgttg
     3541 acaccgtccg tcgcaaacgg aattgggccg cctaccgttt ctacgtggac gatccgaagt
     3601 acctggccac cttcgggctg cacccgtgcg atcctggcaa tgcgtggcag atcaagctgc
     3661 ggctggctga cctggatcaa gccctcaaga gccgaggcga gatggttggg gcggttctca
     3721 tcgttggtgg gcacagcatc gttcccttcc acatgctgcc gaatccgacc gacgacgatg
     3781 acgacctggt accttccgat aacccgtatg ccacttcgga cgagaactac ttcgcgcccg
     3841 agtggccggt gggccgcctg ccgtcggata cagaggtcga tctgctggt
//
LOCUS       DATDPQ010000229         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_366863, whole genome shotgun sequence.
ACCESSION   DATDPQ010000229 DATDPQ010000000
VERSION     DATDPQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366863"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /gene="polX"
                     /locus_tag="VI520_05165"
     CDS             complement(<1..1455)
                     /gene="polX"
                     /locus_tag="VI520_05165"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HLF37306.1"
                     /translation="MNNSEVAQVFDDIADLLEIKGEQIYRVLAYRRGAEALKNLGRDI
                     NTVWKQGELEEIPGVGKAIAGKIDELLGTGKLEFYEELKKEIPLGLLEMLKVGDVGPK
                     KAARFWKELGITSIKALGAAAKRGDLRDLPGMGARSEARILESIQALSRRQTGRLSIG
                     IALPIAEQLLAELRRHPAVKAAEAAGSLRRMRESVGDLDLLVSTDKPEPVMQSFVKLP
                     IIGRVRSQGPTKTSVELVDGLRVQVWTHPSARFGTALQYATGSKEHNVRLREIALDQG
                     LSLSDQAFKLDDGSEITCAQEVEVYKRLGLPWIAPELREDRGEIQAALDGTLPQLIEL
                     SDLKAELHSHSSWSDGANTIDQMVKAARAEGHRLLAITDHSKSLGVANGLTVERLRKQ
                     GKEIAALRRRLPKGFTLLHGSEVEILADGRLDYPDDVLAELDLVIASLHTSLRQSREK
                     VTARLLAAIANPNVDIIAHPTGRLIGEREGADLDM"
     gene            1560..2627
                     /locus_tag="VI520_05170"
     CDS             1560..2627
                     /locus_tag="VI520_05170"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HLF37307.1"
                     /translation="MFEFSGNLHVHSTLSDGHGTHDQIAEAAIRAGLDFVVVTDHNRR
                     PEGMDGYRFVGERRVLMLIGEEVHDPKRTPEKSHLLVYETRRAMAEFGTDPQLLIEQV
                     GSHGGLSFLAHPFDCAAPRFKQGDLSWDDWQVEGYTGLELWNFMVDYKCRLRSLPEAL
                     LFSYLPSRIARGAPRRALALWDSLLSRGKRIVAIGNSDAHALPAKLGPLRRTLFPYAW
                     LFRAVNTHVLSEQALSGELEADRAILFTAIRHGHCFIGYDLAANTRGFMFSGHSEHGN
                     AVMGDGLTFSLGATLQVRCPVPGRIRILQAGNIIREWPASEHAVLTISQPGPYRAEVN
                     LPSGNSFRPWIYSNPIFAERI"
     gene            2669..>3442
                     /locus_tag="VI520_05175"
     CDS             2669..>3442
                     /locus_tag="VI520_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HLF37308.1"
                     /translation="MSEFRLHAPFAPTGDQPEAIRLLAEGLRRGDQHQVLLGATGTGK
                     TFCVASLIAELQRPTLVMSHNKTLAAQLYAEFKDFFPDSAVEFFVSYYDYYQPEAYVP
                     KHDLYIEKETDINEDIERLRLSATASVLSRRDVVVVASVSCIYGIGNPEAYGRIVIHL
                     EQGGVYRREAVLRQLVEGHYARNDITLKPGVFRVRGDTLEVVPAYQEHGLRIGFFGDE
                     LERIVEFDYLTGELLAEHQAIDIFPAKHFITEQEKLQAAI"
BASE COUNT          606 a         1110 c         1034 g          692 t
ORIGIN      
        1 catgtccaag tcggctcctt cccgctcacc gatcaatcgg ccggttgggt gggcgatgat
       61 gtccacattc gggttggcga tcgcggcgag caggcgagcc gtaacctttt cccgactctg
      121 acgcaatgag gtgtgcaagg aggcgatcac caggtccaac tcagcaagca catcgtccgg
      181 gtaatccaat cgcccatccg ccaggatctc cacttccgaa ccgtgcagca acgtgaaccc
      241 tttcggcaat cgcctacgca gtgccgcgat ttccttgccc tgcttgcgga gtcgttcgac
      301 ggtcaggcca ttggcgaccc ccaggctctt tgagtgatcg gtgatagcaa gcagacggtg
      361 cccctccgcc cgagccgcct tgaccatttg atcgatggtg ttggccccat cgctccagct
      421 cgagtgggag tgcagctcgg ccttgagatc cgagagctcg atcaactgcg gaagagtgcc
      481 gtccagcgcg gcttggattt ctccccggtc ttcccgcagc tcgggggcga tccagggcag
      541 ccccagccgc ttgtagactt cgacttcttg ggcgcaggtg atctcactcc cgtcgtcgag
      601 tttgaaggct tgatccgaga gcgacagccc ctgatccaag gcgatttccc gtagccgaac
      661 gttgtgttct tttgagccgg tggcgtactg cagcgcggtg ccgaaccggg ccgaggggtg
      721 ggtccaaacc tggacccgca gcccgtccac caactccaca ctggtcttgg tcggcccctg
      781 gctgcgcacc cggccgatga tcggcagctt gacgaaggac tgcatgaccg gctcgggttt
      841 gtcggtcgag acgagcaggt cgagatcgcc aacgctctcc cgcatgcggc gcagacttcc
      901 ggcggcctcg gccgccttca ccgccggatg gcggcgcagc tctgccaaca gctgctcggc
      961 gatcggaagt gcaatcccga tcgaaagccg gccggtctgc cggcggctta gggcctgaat
     1021 actctccagg attcgggcct cggagcgcgc tcccatcccc gggaggtcgc gaaggtcacc
     1081 tcgcttggct gcggctccca gggctttgat cgaagtgatc ccgagttcct tccagaaccg
     1141 ggcagccttc ttcggaccga cgtctcccac tttgagcatt tcgagcaaac ccagagggat
     1201 ctccttcttg agttcttcat agaattcaag ttttccggtc ccgagcagct catcaatttt
     1261 gccggcgatc gctttgccta cccccgggat ctcctccagc tcgccctgct tccagacggt
     1321 gttgatgtcg cgccccaggt tcttgagggc ttccgccccc cgccgatagg ccagcacccg
     1381 gtagatctgt tcgcccttga tctcgagcag atcggcgatg tcgtcgaaga cctgagccac
     1441 ttcgctgttg ttcatcggat cgcagatccc gggcggacct gccgattcta agcgatccgc
     1501 tgcccgcccc ccggcaatcc agggaatggg tcgcatgata tgcttcgccg ggacccacta
     1561 tgtttgaatt cagcggcaat ctgcatgtcc acagcactct gtcggacggc cacggaaccc
     1621 atgatcagat cgccgaagcc gcgattcggg ccgggctcga tttcgtggtg gtcactgacc
     1681 ataaccggcg accggagggg atggacggtt atcgttttgt cggcgaacgg cgggtcctga
     1741 tgctcatcgg ggaagaggtt cacgatccga agcgtactcc cgagaaatcg cacctcctgg
     1801 tctacgagac tcgccgagct atggccgagt tcggaaccga ccctcaactt ctcatcgagc
     1861 aagtggggag ccatgggggt ctgtcgttcc tggcccaccc gttcgattgt gctgcgccgc
     1921 ggttcaagca gggtgatctc tcctgggatg actggcaggt cgagggctat acgggactcg
     1981 agttgtggaa tttcatggtt gactacaagt gccggctgcg cagcttgccg gaagcgctcc
     2041 tctttagcta ccttcccagc cggattgccc gcggagcgcc gcgcagggcg ctggccctct
     2101 gggactcact cctcagccgc ggcaagcgga ttgtggccat cgggaactcc gacgcccacg
     2161 cccttcccgc caagttgggc ccgctgcgcc ggacgctgtt cccttatgcg tggctgtttc
     2221 gggctgtgaa tacccacgtg ttgagcgaac aggccctgag cggcgagctt gaagcggacc
     2281 gggcaatcct gttcacagcc attcgacacg gccactgctt cattggatat gatctggccg
     2341 ccaacacccg aggcttcatg ttcagcggtc actccgagca tggaaacgca gttatggggg
     2401 acgggctgac tttcagcctg ggagcgaccc tccaggtccg gtgcccggtc cctggccgga
     2461 tccgaatcct gcaggctgga aacatcatcc gtgaatggcc cgcgtccgaa cacgccgtcc
     2521 tgacaatcag ccagcccggg ccctaccgag ccgaagtcaa ccttcccagc ggcaatagct
     2581 tccggccgtg gatatacagc aacccgattt tcgctgagcg catctagctt cggtcggctc
     2641 tcccatcggt gatatagtcg gcggcgcgat gtccgaattc cgattgcacg cgcccttcgc
     2701 gcccaccggc gaccagcccg aggcgatccg cctgctcgcc gaagggctgc gccggggaga
     2761 tcagcatcag gtcctgctcg gggcgaccgg caccgggaag accttctgcg tggccagcct
     2821 gatcgccgag ctgcagcgcc ctaccctggt tatgtcccac aacaagaccc tggcggcgca
     2881 gctctacgcc gaattcaagg acttcttccc agacagcgcg gtcgagttct tcgtctccta
     2941 ctacgactat taccagccgg aggcctacgt cccgaaacac gacctttata tcgagaaaga
     3001 gaccgacatt aatgaggaca tcgagcgttt acggttgtcg gcgacggcct cggtgctctc
     3061 ccgccgcgac gtcgtggtgg tggcctcggt atcctgcatt tacgggatcg ggaaccctga
     3121 ggcctacggt cggatcgtga tccacctcga gcagggtggg gtctatcgca gagaggcggt
     3181 cttgcgccag ctggtggagg gccactacgc tcggaacgac attacgctca agccaggagt
     3241 ttttcgtgtg cggggggaca ccctcgaggt ggtccccgcc taccaagagc atgggttgcg
     3301 gattgggttc ttcggagacg agcttgagcg gattgtcgag ttcgactacc tgactggaga
     3361 gctgctagcc gagcatcagg cgatcgacat cttcccggcc aagcacttca tcaccgagca
     3421 agagaagctc caggccgcaa tc
//
LOCUS       DATDPQ010000230         4294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3265610, whole genome shotgun sequence.
ACCESSION   DATDPQ010000230 DATDPQ010000000
VERSION     DATDPQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265610"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VI520_05180"
     CDS             <1..424
                     /locus_tag="VI520_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF37309.1"
                     /translation="SLGRKPTPEEIAEDLDMDPRKVQWMMRVSWRPLSLETPVGEDED
                     SELGSFVEDETTPTPTQSAYANLLRDKIEEVLTTLSPREARILRLRFGLHNGRSYTLE
                     EVGQKFGLTRERIRQIEGKALRRLRHPRRSRQLRDYLS"
     gene            complement(542..1051)
                     /locus_tag="VI520_05185"
     CDS             complement(542..1051)
                     /locus_tag="VI520_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HLF37310.1"
                     /translation="MPKSVHSRPMNESLEIEVGSALRQKGWTLALGESCTGGLVAHRI
                     TEVAGSSEYFLGGVVAYSNPVKHGLLRVPEQTLERHGAVSEETATQMARGARAVLGAD
                     VGLSVTGIAGPGGGTTEKPVGLTVLAVSTPVGEWIERHVWNGDRHANKQASAEAALRL
                     LLRALSTEP"
     gene            1125..2348
                     /gene="xseA"
                     /locus_tag="VI520_05190"
     CDS             1125..2348
                     /gene="xseA"
                     /locus_tag="VI520_05190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLF37311.1"
                     /translation="MSVLSLFGLQSAIWSPSQLNRHIRQVIESDYRLSDLWLAGEVAN
                     LSHPASGHLYFSVRDSEAAVRCVMWRPEVARLRRLPRDGEAVEAHGHLSVYEAGGQVQ
                     LYADQIRFAGEGELYQEFLRLKAALEAEGLFDSDRKRTLPAWPRRIGVVSSPTGAALR
                     DVANVLSRRYPLAELILSPTPVQGEAAPAAIVEALRAVNEYGRPDVILLVRGGGSMED
                     LWPFNTEAVVRAVVASAAPVVTGIGHETDVNLADFAADRQAPTPSAAGEISTPDRAEL
                     AAELTQIRQRLARAWRQQRRALHSQLAEQRVALGLGSPQARIANARQRSDDLARRLGA
                     AVRHELALRQAALRGLVQTLSAVGPETILARGYAVVRKLADGEVVRSLTQVRAGDPLG
                     VRVSDGSFGARVEGN"
     gene            2354..2602
                     /locus_tag="VI520_05195"
     CDS             2354..2602
                     /locus_tag="VI520_05195"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HLF37312.1"
                     /translation="MAKKKPVDQLRFEEAFEELAALVEQLEGGEQPLEESLALFERGQ
                     ALAAHCSQLLEQAELKLRQLAPDASGGYSESDLEIEAE"
     gene            complement(2651..4210)
                     /gene="murJ"
                     /locus_tag="VI520_05200"
     CDS             complement(2651..4210)
                     /gene="murJ"
                     /locus_tag="VI520_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HLF37313.1"
                     /translation="MAPLAERQIARSAGTVMVGFGLSSLLGLANRMLYTRAFGSGQEL
                     DAFLAANRVPDILFNLMAGGALASAFLPTFSALLAQGDQRRAWHLASSIGNLLILALG
                     LAAGLLGLVAPWLVENILGRGFHDPQQVQLTVSLLRIQLVAPVLFGISGLLMAILNAQ
                     QHFLLPALAPTFHWLGWILGSLFLVPRLGISGLAWGVLVGAVLHLSIQLPGLRRVRPQ
                     YAATFGLQDPSVRQVGRLMAPRLFGVAIVQLNFLVNVILASGMAVGSLTGITLGFAIM
                     LMPQIVIAQAVAIAALPTFSSQAAKGELVEMRRSLSSTLRGVIFLSLPASLGLILLRV
                     PLVTLLFQRGEFDSNSTALVAWALLWYSTGLVGHSLVEIVSRAFYALKDTRTPVTVGA
                     AAMALNVVLSIGLSRLFLRLGWAPHGGLALANSIATGLEAIALLWLVSRRLEGIEIVR
                     IRPGIAASLLATAAMGFGLMLWLAVTEGRSAWMVALGGVGLGAAAYWLASLGLRSPEA
                     RQLPRLILARR"
BASE COUNT          831 a         1361 c         1394 g          708 t
ORIGIN      
        1 gtcactgggt cgaaagccca cccccgagga gatcgccgaa gacttggaca tggatccccg
       61 caaggtgcag tggatgatgc gggtctcctg gcggccactt tcgttggaga ccccggtggg
      121 ggaggatgaa gattccgaac ttggctcttt tgtggaggac gagacaaccc cgacccctac
      181 ccagagcgcc tatgccaatc tgttgcggga caagatcgag gaggtgctta ccaccctcag
      241 cccgcgggaa gcccgcatct tgcgcttgcg ctttgggctg cacaacgggc gctcatatac
      301 cttggaagag gtcggccaga agtttggcct gacccgggag cggatccgtc agatcgaggg
      361 gaaggcgctc cgccgcctgc gccacccgcg ccgcagtcgg cagctgcggg actacttaag
      421 ctgagtcgag tgagtttgag atcagtgccg ggcggggaac tccccgcccg gtttcattaa
      481 ccgcgtcgca gacctgtttc gatctccgac ccgggccgag atcttccggc cggcggccga
      541 ctcagggttc cgtgctaagg gcgcgcagca gcagccggag ggcagcctcc gcggaagcct
      601 gcttgttggc gtgtcggtcc ccattccaca cgtgccgttc gatccactcg cccaccggag
      661 tgctgaccgc cagcacggtc agccccaccg gtttctcggt cgttcctccg ccggggcccg
      721 cgattccggt taccgatagt ccaacatccg ctcccaggac cgcccgagcg ccgcgggcca
      781 tctgggttgc ggtttcttcg ctgactgcgc cgtggcgctc tagggtttgc tcaggcaccc
      841 gcagcaagcc atgtttcacc gggttggagt aggccacgac tccccccagg aaatactccg
      901 atgacccggc gacttcggta atccggtgag caaccaggcc tccagtgcac gattcgccca
      961 aggccaaggt ccaacccttt tggcgcagtg cgctcccaac ctcgatctcc aacgattcgt
     1021 tcatcggtcg gctgtggact gacttgggca ttataacgtt gcccctttcc cttcccgcgg
     1081 cggttcaatt gacaccccct ccagcggagg gtatccttcg cgtgatgagc gtgctctcgc
     1141 tcttcgggct gcaatccgcg atctggtcgc ccagccaact caatcgccac attcgccaag
     1201 tgatcgaaag tgactatcgg ctgagcgacc tgtggctggc aggtgaggtc gcgaatctct
     1261 cccatcccgc ctctggccat ctgtatttca gtgtgcggga ttcggaggcg gccgtccgct
     1321 gcgtgatgtg gcggcccgag gtggctcggc ttcgccgctt gccgcgtgac ggcgaggcgg
     1381 tcgaggccca tgggcatctg agtgtttacg aggccggcgg gcaggttcag ctctatgccg
     1441 accagatccg gtttgccggc gagggcgagc tctatcagga attcctgcgg ctgaaggctg
     1501 ccctggaggc cgaagggctc tttgattcgg atcgcaaacg aaccttgccg gcgtggccca
     1561 ggcggatcgg agtggtcagc tccccgaccg gagccgcgct gcgcgacgtg gccaatgtgc
     1621 tgagccgccg atatcccttg gcggagctga tcctctcgcc aaccccggtc caaggagaag
     1681 ccgcgcctgc ggcaatcgtg gaggcgctgc gggcggtcaa cgaatatggc aggccggatg
     1741 tgatcttgct cgtccgggga ggcggctcga tggaagacct gtggccgttc aacaccgaag
     1801 cggtggtccg agcggttgtg gcttcggcgg ccccggttgt cactgggatc gggcacgaga
     1861 ccgatgtgaa tttggccgat ttcgccgccg atcggcaagc gccaactccc tcggcggcgg
     1921 gcgaaatctc cacgcccgac cgggccgagt tggcggcaga gctcacccag attcgccaac
     1981 ggctggcgag ggcctggagg caacagcgta gggcgcttca ctctcagctt gcggaacagc
     2041 gggtggcctt ggggttgggt tcgcctcagg cccggattgc caacgctcgt caacgcagcg
     2101 acgatctcgc ccgccggtta ggggccgccg tgcgacacga gctcgccctg cgccaggctg
     2161 ccctgcgggg tctggtccag accctgagtg cggtagggcc cgagacgata ttggcgcgtg
     2221 gctacgcagt cgttcgaaag ctggcggacg gggaagtcgt gcgctcgctg acccaggtga
     2281 gagccgggga cccgctcggg gttcgggtga gcgacggcag ctttggggcc cgagtggagg
     2341 gcaactgacc gatatggcga agaagaaacc ggtcgatcaa ttgcgctttg aggaggcttt
     2401 cgaagaactg gccgccctgg ttgagcaact cgagggcggg gagcagccgt tggaagaaag
     2461 tctggcgctc ttcgaacgcg gtcaggcgtt ggcggcccac tgcagccagc tgctcgaaca
     2521 ggcggagctg aagcttcgcc agctggcacc ggatgcgagt gggggttact ccgagtcgga
     2581 cctcgaaatc gaagccgaat agctcagatc agaccagacc agcacccggc cgatccgggt
     2641 cttcggccaa tcagcgccgg gcgagaatca atctcggaag ttggcgggcc tccggcgatc
     2701 tcaaccccag cgaagccagc caataggccg cagcccccaa ccccaccccg cccagcgcca
     2761 ccatccaggc cgatcttccc tcggtcacag ccagccacaa catgagaccg aagcccatcg
     2821 cagcggtggc cagcaggctg gcggcgatgc cgggtcggat ccggacaatc tcaattccct
     2881 cgagccgacg gctgaccagc cagagcaggg cgatcgcttc caacccggtc gcgatcgaat
     2941 tggcaagtgc caatccccca tggggagccc aaccgagccg gaggaagagc cgcgacaaac
     3001 caatactgag cacaacgttc aatgccatcg cggccgcgcc gacagttacc ggagtgcggg
     3061 tgtccttcag tgcgtagaag gcccgcgaga cgatctccac cagggaatgg cccaccaagc
     3121 cggtcgaata ccaaagcagg gcccaggcta ccaaagctgt cgagttggaa tcgaactccc
     3181 cgcgttggaa gagcagggtt accagcggaa cccgcaacag gataagaccg aggctggccg
     3241 gcaaggatag gaagatcacc ccgcgcagcg tactggagag cgagcgacgc atctccacca
     3301 gttccccttt cgcagcctgg cttgaaaagg tcgggagcgc cgcgatcgcg accgcctggg
     3361 cgatcacgat ctggggcatc agcatgattg caaagccgag ggtgatcccg gtgagactgc
     3421 ccactgccat cccagaggcc aggatcacat tgaccaggaa attgagctgg acgatcgcca
     3481 cgccgaagag tcggggcgcc atcaagcggc ccacctggcg gacagaagga tcctgcaacc
     3541 caaaggtggc cgcgtactgc gggcggaccc gccgcagtcc gggcagctgg atcgacaggt
     3601 gaaggaccgc gccaaccagc actccccagg ccaacccgct aatcccgagc cgcgggacca
     3661 ggaacaggct gcccaggatc cacccgagcc agtgaaaagt gggagccaag gccggaagca
     3721 ggaagtgctg ctgagcatta aggatggcca tcagcaatcc actgatccca aagaggaccg
     3781 gagcgaccag ttggatgcga agcaagctga ccgtgagctg gacctgctgg ggatcgtgga
     3841 acccgcgccc caggatgttt tcgactagcc agggagcaac caatccaagc agcccagccg
     3901 ccagccccaa ggccaggatc agcagattgc cgatactcga ggccaaatgc caggcgcgtc
     3961 tctgatcgcc ttgggcgagc agcgccgaaa acgtcggcag gaaggctgag gccagcgctc
     4021 cgcccgccat caggttgaag agaatatcgg gcactcggtt ggcggctaag aaggcgtcca
     4081 gttcttggcc gctgccaaag gcccgggtgt acagcattcg gtttgccaac cctaagaggc
     4141 tggacaagcc gaaacccacc attaccgtgc ctgcgctacg ggcgatttgc ctttcagcaa
     4201 ggggggccat ggggtcggat tatacaggga gggtcgctga cactttcgcg atccggtggg
     4261 gttgtaaagg gcgtcgttac ccaaatcacc taaa
//
LOCUS       DATDPQ010000231         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_889731, whole genome shotgun sequence.
ACCESSION   DATDPQ010000231 DATDPQ010000000
VERSION     DATDPQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889731"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VI520_05205"
     CDS             <1..472
                     /locus_tag="VI520_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37314.1"
                     /translation="GAGSFLGPGLATIVWPGGLRLVRRSAKITSPMEETKSPISGIWL
                     LMALIVGVLIFGDLTRRMTDARRMERDGRMLATEVSQLGAANSELETQIAQTSDEANV
                     ARWARAEAKLVLEGERLVVPLPAERLAEEQPTAADRAQDAPSTWEIWWALLVGG"
     gene            535..606
                     /locus_tag="VI520_05210"
     tRNA            535..606
                     /locus_tag="VI520_05210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Met,seq:cat)
     gene            complement(743..1147)
                     /locus_tag="VI520_05215"
     CDS             complement(743..1147)
                     /locus_tag="VI520_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLF37315.1"
                     /translation="MQDLLDRFVDRTLVGDPWFEGGYLGRLPLDVYTTDEAWIVKANV
                     PGFKPEEIEISVTGDTLTIQGETKQELESNGDEGKYLVRERRMVNFARSLTLPGKLEA
                     EEAKAEFENGVLTLTLPKPEQIKPKRISVKSK"
     gene            1342..>2723
                     /locus_tag="VI520_05220"
     CDS             1342..>2723
                     /locus_tag="VI520_05220"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HLF37316.1"
                     /translation="MRSGPADSKAEEGERMVELSGQDLTIEQVVAVARNGEPVKGLPD
                     RALDRMRASRAWVEQAVRDDAGPVYGVTTGFGPLAQVRIAPEQARRLSRNLILSAVVG
                     VGSPLPADIVRAMLLIRVNTFANGLSGVRPVLAETLIRMLNSGVTPYVPAKGSLGASG
                     DLAPLAHIAVVLSKDPDQPADGDSGRAWFGGELMSGAEAMKRAGIERVELGAKEGLGM
                     TNGTNLMAAAAALGVYDAVNLLRHAEIAAALSFEALLGTSSALDDRLHRANSQPGQTA
                     TAATLRRLLEGSRLVDSRPGRVQDAYSLRCTPQVLGPVRDVLAFLRERVIGAINAATD
                     NPLFFGDSGAISGGNFHGQGLALWLDILGVAAAQLGTISERRTFRMTTPELSEGLPSM
                     LVLNAGLDSGLMMPQYTAAALVSDNKTLAHPDSVDSIPSSANQEDHVSMGANAARHAL
                     EILENVRLVLA"
BASE COUNT          476 a          836 c          881 g          530 t
ORIGIN      
        1 aggggccggt tcttttcttg gcccggggct tgcaacgatt gtctggccgg gcggcttgcg
       61 gttggtgcgc cgctccgcta agatcaccag cccaatggaa gagacaaagt ccccgatctc
      121 cggtatctgg ctgctgatgg ctctcatcgt cggagtgctc atctttggcg acctcactcg
      181 caggatgacc gatgcccggc ggatggagcg cgatgggcgg atgctggcga ccgaagtcag
      241 ccagctgggg gccgccaact cggagctcga aactcagatc gcccaaacca gcgacgaggc
      301 caacgtcgcc cgttgggcgc gggccgaggc caagcttgtc ttggaaggtg aacggctggt
      361 cgttccgctt ccggccgaac ggttggcgga ggagcaacca accgcggccg accgtgcgca
      421 ggatgcccca agcacgtggg agatttggtg ggcgctgctg gtgggtggtt agcgggccga
      481 ctggctcgat tcccatgata taatccgccc cgcgccagga tctggcgcac ccgctgcggg
      541 gtggagcagt ggcagctcgt caggctcata acctgaaggt cgcaggttcg aatcctgccc
      601 ccgctacctg cattgaccaa gggccgtcct gaatgggcgg ccctgcgcat tccagcctgc
      661 agcagccccc gattggttca tgattaacaa agcgcctgag gcacttgcct caggcgctcg
      721 ttgagtctga actccgaaga gcttacttgc tcttcacaga aatccgcttg ggcttgatct
      781 gctccggttt cggcagggtc aaggtcaaga ctccgttttc gaactctgcc ttggcctcct
      841 cagcctcgag cttgcccggc agggttaacg accgagcaaa gttcaccatc cgacgctccc
      901 ggacgaggta cttgccctca tcgccgttgg attcgagctc ctgctttgtt tcgccctgga
      961 tggtaagcgt gtctcccgtg accgagatct cgatctcctc cggcttgaaa ccggggacat
     1021 tcgctttcac aatccaggcc tcgtccgtcg tgtagacgtc gagcggcagg cgacccaagt
     1081 atcccccctc aaaccaggga tcgcccacaa gggtccggtc aacgaaccgg tcgagcaggt
     1141 cttgcattct ccgcgattcg cggattggtt cccaacgcat caagcttgtc ataatccacc
     1201 tccagatggt ttgttgctct gctaaccact ctaggtgacc cgcatcagaa gaccaatgga
     1261 agttcatcag aggagggtta attccttgac cttaagaccc gaagtacaat ccgggcagcc
     1321 ggggagcctg ctgtcggata tttgcgatcc gggccggctg actccaaggc cgaagagggc
     1381 gaacgcatgg tggagttgag cggccaggat ctgactatcg agcaggtcgt ggccgtcgcc
     1441 aggaatgggg agccggtgaa gggcctgccc gaccgggcgc tggaccgtat gcgagccagc
     1501 cgagcctggg tcgagcaggc ggttcgcgac gacgcgggtc cggtctatgg tgtgacgacc
     1561 ggatttgggc cgcttgcgca agttcgtatc gcccccgagc aggcccgccg gctctctcgc
     1621 aatctgatcc tatctgctgt ggtcggggtt gggtcgccgc tgccggcaga catcgtgcgt
     1681 gccatgctgc tgatccgggt gaacaccttc gccaacggtc tctcgggagt gcggcccgtc
     1741 cttgcggaaa ccctgattcg gatgttgaac tccggtgtaa ccccctatgt tccggccaag
     1801 ggatcgctcg gcgccagcgg cgacctcgca ccgctggcgc atatcgccgt ggtgctctcc
     1861 aaggaccccg atcagccggc agatggagac agcggccggg cctggtttgg cggtgagctg
     1921 atgagcgggg cggaggcgat gaaacgggcc gggatcgaac gagttgagct gggggctaaa
     1981 gagggcctgg gcatgaccaa tgggaccaat ttgatggctg cggcagccgc gctgggggtc
     2041 tatgacgcgg tgaacctgct gcggcatgcc gagattgcgg ccgccctcag cttcgaggcg
     2101 ctgcttggga ccagctccgc cctcgacgac cggctccacc gggccaacag ccagcccggc
     2161 cagactgcga ccgccgcgac tctccggcgc ctgctcgaag gaagccgtct cgtcgactcg
     2221 cgccccggcc gagtccagga cgcctacagt ttgcgctgca ctccccaagt gcttgggccg
     2281 gtcagggatg tattggcatt ccttcgtgag cgagtaatcg gagcgatcaa cgctgcgacc
     2341 gacaaccccc tcttcttcgg ggactccggc gcgatctcgg gcggaaattt tcatggacag
     2401 gggctcgcat tgtggctgga tatcttgggg gtcgcggcgg ctcagctcgg gacgatctca
     2461 gagcgacgga cgtttcgaat gaccactccg gagctcagtg agggcctgcc gtctatgttg
     2521 gtactcaatg ccgggctgga cagtggattg atgatgccgc aatacaccgc ggcggccctg
     2581 gtctcggaca ataagacctt ggctcatccc gattcggtcg actccattcc ttccagcgcc
     2641 aaccaggagg accacgtcag tatgggggcc aacgcggcgc ggcatgcgct ggagattctc
     2701 gagaatgtgc ggctggttct ggc
//
LOCUS       DATDPQ010000232         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_623015, whole genome shotgun sequence.
ACCESSION   DATDPQ010000232 DATDPQ010000000
VERSION     DATDPQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623015"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="VI520_05225"
     CDS             complement(<1..1683)
                     /locus_tag="VI520_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37317.1"
                     /translation="MVRSILPRNLWSGLLFGGALSVALAAAVWAGFTGPNRTTTQEVR
                     DPGSEFWYCTRGGVTCEFASGVNPCPPFGSHPSAPAVLSVCGWSSGYSCGCDAAFTEE
                     TISLPPATVSGSAACSVPGGAGWCRGGASVNLSASEPLAGEVIELIEGNPAGVLCDPA
                     DGPSVSCSWSGSDGDFTLQFWAVSSYGDTSDLGSSNWRVDSGAPSVSLNLSGGTLGGG
                     GWYRGGTVSVSASGADALSGVAISEVAIDGGSWTGTGQVSSDGVHSASGRVRDVAGNE
                     ATGTASIRYDSTPPTLSADLSGTLGREGWYISSVSASAAASDSLSGVAERQVNVNGTG
                     WQTGPILLTQDGSLSLSFRAADVAGNEAISGAQTIRIDAHPPESVFVVPPNGSETWVS
                     GTVELLGQSLDFGSGLRSVEISYDGGQTWAPLTLERSEWRDNWDTKSVPNGTYVVLAR
                     ASDVAGHLESTARVTLHVDNLPPFVDIPDSWLVGERAPLVVKEQGVGLAGVQILIQDG
                     EQALFAWPYDPELIPGTISWDGVMPDGSVAAPGDYGVSATAWDLAGNRGQDVG"
     gene            1797..2999
                     /locus_tag="VI520_05230"
     CDS             1797..2999
                     /locus_tag="VI520_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HLF37318.1"
                     /translation="MGTSIRSSITFALATTAIALVGCAQPGPATIPTAPAAVAQAAVA
                     LAPVLPAATSAVLAPTPVPQVASLPTAAPAEQPGEAAPTSSRSLFDLLPGESLKLLED
                     IPIARTDFGVRVAFSPDSDVVLHNGNGLVIQQFDLAQSEPLQDLIGFELFSPFTISVS
                     SDGSTVVADDVSQLHVWSVASGERVDSLALPPVSGVMTAGFHADEFFFAADFHGNVIL
                     WDPHGWGEISRFTHPGQIDAAVLFPDGSAIALQDRRQREIGIFDLQGTRLGTLPIEHE
                     NDLLLSISPAGDRFLLHVDYGYPTEGIRIIDVASGETLLDLPLLNFRQFAVSSDWSLL
                     AAVGVQNELRLYRLPDGQLLLAQPLEVARTLGISMSGDAAYLAFYVFKSGNAGAAIQV
                     WGRGSDRP"
BASE COUNT          603 a         1068 c          899 g          591 t
ORIGIN      
        1 cccaacatcc tggccccggt tcccagcgag atcccaagcg gtggcagaaa ccccgtagtc
       61 accaggtgca gcaacgctgc catcgggcat cactccatcc caactgatcg tgcccggtat
      121 gagctccgga tcgtatggcc atgcgaatag tgcctgctcc ccatcttgga tcagaatttg
      181 aacacccgcc aggccgaccc cttgctcctt gacgaccagc ggggcacgct cgccgaccag
      241 ccaagagtca ggaatatcca caaagggcgg caggttatca acgtgcagag tgaccctggc
      301 tgtagattcc aagtggccgg caacgtcact tgcccgggcc aagacgacat aggtgccatt
      361 cggcaccgac ttggtatccc agttgtccct ccattcggaa cgctccaacg tgagcggagc
      421 ccaggtctgg ccaccatcgt acgagatttc gaccgaacgc aatccggagc caaaatccag
      481 gctttgaccc aacaactcca cggtgccgga gacccacgtc tccgaaccgt ttggcgggac
      541 aacgaacacg gactctgggg gatgagcgtc gatccgaatg gtctgagccc cagagatcgc
      601 ttcgtttccg gcaacatcgg ccgccctgaa acttaagcta aggctcccgt cctgggtcaa
      661 caggatcggg ccagtttgcc atccggtccc gttgacgttc acttggcgct cagccactcc
      721 cgataaacta tcggaggccg cggcggaggc actcaccgag gagatatacc aaccctcccg
      781 accgagtgtc ccgctgagat cggccgagag ggtgggcgga gtgctgtcgt atcggatcga
      841 agctgtgccg gtggcttcgt ttccggcgac atctcggacc cggccgctgg ccgaatggac
      901 tccatcacta ctgacctgac ccgtcccagt ccacgaaccg ccgtcaatcg cgacctcgga
      961 gattgcgacc cccgagagcg catcggcgcc gctcgccgac acgctgactg ttccaccccg
     1021 ataccatccg cccccaccca gcgtcccgcc gctcagattc aggcttacgc tgggcgcacc
     1081 cgagtcgact ctccagttcg acgaccccag atcgctcgtg tcgccgtagg acgacactgc
     1141 ccagaattgc agagtgaaat cgccgtcgct gccagaccag gagcaggata cacttggtcc
     1201 gtcggccgga tcgcacagca ctccggccgg attgccctcg atcaattcga tgacttcgcc
     1261 ggccaggggt tcgcttgccg agaggttgac ggacgcacca ccccggcacc agcccgcgcc
     1321 gcccggtacg ctacaggcgg ccgaaccgct gacggtcgcg ggcggcagac tgatcgtttc
     1381 ctcagtgaag gccgcgtcgc agccgcagct gtatccgctc gaccaaccac agacggaaag
     1441 taccgcggga gcgctcggat gagacccgaa tggagggcaa ggattgacac cactggcgaa
     1501 ctcgcaagtc actccgcccc tcgtgcagta ccagaactcg ctgcccggat cgcggacttc
     1561 ttgggtcgtg gtccgattgg gaccggtgaa tcctgcccaa accgctgccg ccagcgcgac
     1621 agagagcgct ccaccaaaga gcagcccgct ccaaaggttt cgaggtaaga tgcttctgac
     1681 cacagctcag gtagtgctag agtactacat taagacccag aatcggctcc cagatggagc
     1741 atgaccccag tactagttcg ataactatac tctaagcgat gcagagtcct tcgagagtgg
     1801 gaacgtcgat aaggagctca atcacatttg cgctggccac gacggctatt gccctggtcg
     1861 gctgcgcaca gcccggaccg gccaccattc ccactgcgcc agccgcggtg gcccaggcgg
     1921 ccgtcgcgct cgcgcctgtc ctccccgcag ccacttccgc cgtcctggcc ccgacgccgg
     1981 ttccgcaggt cgcctcactg ccaaccgcgg cgcccgccga gcaaccgggc gaggctgctc
     2041 ccacttcgag ccgatcgctc ttcgatctcc tgccaggtga aagtctcaag ttgctggagg
     2101 acatcccgat cgcacgaact gacttcgggg tccgggtagc tttttcaccg gacagcgacg
     2161 tggtgctcca taatggcaac ggcctagtga tccaacaatt cgatctggcc cagagtgaac
     2221 ctctccaaga cctgatcggc ttcgagctgt tttccccgtt tactatttca gtttcatccg
     2281 acgggtccac ggtcgtcgcc gacgatgttt cacaattaca cgtctggagt gtcgcttccg
     2341 gcgagcgggt tgattctctc gctcttcccc cagtctctgg cgtcatgaca gcagggttcc
     2401 acgccgatga attcttcttt gctgcggatt ttcacgggaa tgtgatcctg tgggatcccc
     2461 acggttgggg agagatctcc cgcttcacgc atccggggca gatcgacgcg gccgttcttt
     2521 tccccgacgg ttcggcaatc gcgctgcagg atcggcgcca gcgggagatt gggatctttg
     2581 atttgcaggg cacccgcctc gggacgcttc cgatcgagca cgagaacgac ttgttgcttt
     2641 cgatctcgcc cgccggtgac cgcttcctgc ttcacgtcga ttacggatac ccgaccgagg
     2701 gaatccggat cattgacgtg gcgagtggcg agaccttgct cgacctaccg ctcctgaact
     2761 tccggcaatt cgcagtgtcc agcgactgga gtttgctggc cgcggtcggc gtccagaacg
     2821 agctgcgttt gtatcgcctg ccggatggac agttgctgct ggctcagccg ctcgaagtgg
     2881 cccgcaccct cgggatcagc atgtctggtg acgctgccta cctggcgttt tacgtgttca
     2941 agagcggcaa cgccggcgct gccattcagg tctggggccg cggcagcgac cggccctaag
     3001 cctcaccccc cgcctagaaa gctcgcggca gtccctcggt ggtaaactcg ccgcccatgc
     3061 gcgtcagaga agccgtcgcg gcggcccacc ccaacatcgc tctggtcaag tactggggta
     3121 accgagacca cgccctaaga atcccctcca acggttcgat t
//
LOCUS       DATDPQ010000233         3328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_369650, whole genome shotgun sequence.
ACCESSION   DATDPQ010000233 DATDPQ010000000
VERSION     DATDPQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369650"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VI520_05235"
     CDS             <1..795
                     /locus_tag="VI520_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HLF37319.1"
                     /translation="EDRLVKVIVCVKQTPDTAANLTVVGGKASWGDAPLVLNPWDEYA
                     IEQALRTKEAHGGSVTAISLGPEAAKDSLKQALAMGCDEAVLLSDPAFERADSLVASA
                     ALAAAIKRIDASDLVFFGKAAVDTDTGVTGPQVARRLGWPSLNLVAAISELDPAAKTI
                     TVERLLEEGRQIVRSPLPAVVSVVKEINEPRYPSFMGIRKASKAEIPVWTATEIGLLP
                     VPEPALSWPEVTAPARTEQSCEFMSGETAQQQAAALVDRLIEEKLI"
     gene            798..1823
                     /locus_tag="VI520_05240"
     CDS             798..1823
                     /locus_tag="VI520_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HLF37320.1"
                     /translation="MLVYIDHFGGKAPTVAWEALGAGARLAEGSEVKAVVMGQGVEAL
                     AGEVVSRGAHGVVLADDDRLKEFRPEVYADGLSEIIRQSGAATVLFPTTSRARELAAL
                     VAVDLGTGITPDAIQLDRKNGAIVATRPIYAGKLLTKEVNLAHRPQLLTVRSRSFPAP
                     EPDPARAGEITRVELDLGQPRTEVMGYKPSGGGVSLADAAIIVSGGRGVANNPGLQPP
                     AELTSEKEQEIWRAREGFKLVGELAQVLGGAVGASRAAVDAGYIPYAHQVGQTGKVVS
                     PDLYIACGISGAIQHLAGMRTAKTIVAINRDPDAPIFKLARFGVVGDLHQILPALIGV
                     LRQRLGK"
     gene            complement(1854..1925)
                     /locus_tag="VI520_05245"
     tRNA            complement(1854..1925)
                     /locus_tag="VI520_05245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1890..1892),aa:Arg,seq:ccg)
     gene            2023..2928
                     /locus_tag="VI520_05250"
     CDS             2023..2928
                     /locus_tag="VI520_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLF37321.1"
                     /translation="MDRRIGFIGLGLMGKPMSLNLVKAGFELTVHSRSPGPVQELAAA
                     GAAVASSAREVAESSEIVITCLPDSPDVEAVVLGSAGILEGAAPGSLFIDHSTIMPEL
                     ARRISNRLGDHEISALDAPVSGGDVGAKAGTLAIMVGGPPAAFERARPVFEAVGKTIT
                     YVGESGAGQVAKACNQIMVAAQMVAMGELLLLAQKLGVSPVRVVDAIKGGAAQCWTLD
                     VKPARLFAGERRPGFKAHLMHKDLAIALDTARAYGMPLPATAANMQLFEAMLQLGMRE
                     LDNSAVIGVLEALSGEKLQEESRSA"
     gene            complement(2963..>3328)
                     /locus_tag="VI520_05255"
     CDS             complement(2963..>3328)
                     /locus_tag="VI520_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HLF37322.1"
                     /translation="VVQPEVPEGAAGTRAIGAFGEFPVGLTGAVAGLADTGSLILSGG
                     SRRSLLPSLLPEAHLAVVRSKDIYADLEGWLRAVGSPYVAGSSNLVLISGPSRTADIE
                     MTLTMGVHGPRQLIVFLIE"
BASE COUNT          628 a         1045 c         1121 g          534 t
ORIGIN      
        1 gaggacagac ttgtgaaagt tatcgtctgt gtcaaacaaa ccccagatac cgccgcgaac
       61 ctaaccgtcg ttggaggtaa ggcctcctgg ggcgacgccc cgctcgtcct gaatccttgg
      121 gacgaatacg caatcgagca agcgctgcgg accaaggaag cccatggcgg gagcgtcacg
      181 gcgatcagcc tcggtcctga ggcggccaaa gactcactga aacaagcact ggccatgggc
      241 tgcgacgaag ccgtcttgct cagcgatccg gcgttcgaga gagccgactc gctggtcgct
      301 tccgcggcct tggcggcggc gatcaagcgg atcgatgctt cggatctcgt cttcttcggc
      361 aaggcagccg tcgacacgga taccggagtg accggtcctc aggttgcccg ccggctgggc
      421 tggccctcct tgaacttggt tgcagccatc agcgagctcg acccggcggc gaagaccatc
      481 acggtagaac gcctcctgga ggagggccga cagatcgtgc gaagcccgct ccccgccgtg
      541 gtgagcgtcg tgaaagagat caacgaacct cgctacccat cattcatggg gattcgaaag
      601 gcctccaagg ccgagatccc ggtctggacc gcaaccgaga tcggcctgtt gcccgtccct
      661 gagccggctt taagctggcc ggaagtaact gcaccggctc ggaccgagca gagctgtgag
      721 ttcatgtcgg gggagacagc ccagcaacag gccgcggccc tggtggacag gctgatcgaa
      781 gagaagctta tctgatggtg ctggtctaca tcgatcattt cggcggaaag gcaccgacag
      841 ttgcctggga agctttaggg gccggggctc gacttgcgga gggctccgag gtcaaggcag
      901 tggtgatggg ccagggggtc gaggcgctgg ccggcgaggt agtcagccgc ggcgcgcacg
      961 gggtggtgct ggcagacgac gatcgactca aggagttccg gccggaggtc tacgccgacg
     1021 gcctgagcga gatcatccgc cagagcgggg ccgccacggt ccttttccca actaccagcc
     1081 gggcccgcga gctggccgct ctggtggcgg tcgacctggg taccggaatc acccccgacg
     1141 cgatccagct ggaccgaaag aacggcgcga tcgtcgcaac ccggccaatc tacgccggga
     1201 agctgctgac gaaggaggtc aacctcgccc accggcccca gctgctcacc gtccgcagcc
     1261 gatcattccc agcgcccgaa cccgatcccg ctcgggcagg cgagatcacc cgagttgagc
     1321 tggatctcgg ccagccgcgc accgaggtaa tgggctacaa gccgtccggc ggtggggtca
     1381 gcctggccga cgcggccatc atcgtctccg gtggacgtgg agttgcgaac aaccccgggc
     1441 tgcagccgcc agccgagctc accagtgaga aggagcagga gatctggcgg gcgcgggagg
     1501 gtttcaaatt ggtcggagag ttggcgcagg ttttgggagg cgcggttggg gccagccggg
     1561 cggcggtgga cgccggctac atcccctatg ctcaccaggt cggccaaacc gggaaggtcg
     1621 tttctccgga tctctacatt gcctgcggga tatccggagc gatccagcac ctggccggaa
     1681 tgcggacggc caaaacgatt gtggccatca accgggatcc cgacgccccg atcttcaagc
     1741 ttgcccgctt cggcgtggtt ggcgacctgc accagatcct ccccgcgttg atcggagttc
     1801 tcaggcaacg tctgggaaag taaacggccc cggtgggcgg ccggggcctg cagcgcccct
     1861 gcggcgactc gaacgccggc atctgcctcc ggagggcagc gctctatcca ctgagctaca
     1921 ggggctgaag gcgattgtat ccgatgaaag agggggcctc aagccgggca ggccgtgcgt
     1981 gatcaggcag agactaaggg aacaggttag gttgaggtga acatggacag acgaatcggg
     2041 ttcatcgggc ttggcctgat gggaaagccg atgtcgctca atctcgtgaa ggccggcttc
     2101 gagctcaccg tacacagtcg cagccctgga ccggtccagg aattggccgc cgccggcgcg
     2161 gcggtggcca gttcggctcg ggaggtcgcc gagtcctctg agatcgtgat cacctgtctg
     2221 ccggattcgc cggatgtcga ggccgtggtg ctcgggagcg cgggaatcct ggagggagct
     2281 gcgccgggca gcctcttcat cgatcacagt acgatcatgc ccgagctggc tcgtcggatt
     2341 tccaaccggc tcggggacca cgagatttcg gcgctggacg cgccggtatc gggcggcgac
     2401 gtgggcgcca aagccggcac cctggcaatc atggtcggcg gccctccggc ggcatttgaa
     2461 cgggccaggc cggtttttga ggccgtcggc aagaccatca cctacgttgg cgagagtgga
     2521 gccgggcagg tggccaaggc ctgcaaccag atcatggtgg cggctcaaat ggtcgcgatg
     2581 ggagagctgc tgttgctggc ccagaagcta ggagtctcgc ccgttcgagt ggtggacgcc
     2641 atcaagggcg gagcggcaca atgctggacg ctcgacgtca aaccggcccg gctcttcgcc
     2701 ggcgagcggc gacccgggtt caaggcccac ctcatgcaca aggacttggc catcgctctc
     2761 gacacggcgc gggcctacgg gatgccgctg cccgcaactg cggcaaacat gcagctcttt
     2821 gaagcgatgc tgcagctcgg gatgcgcgag ctggataact ctgccgtgat cggggttctg
     2881 gaggcgctct cgggcgagaa actgcaggaa gaatcccgct ccgcctgagg gagccatgag
     2941 gctctccggg cggcagcaac aactactcga tcaggaagac aatcagttgc cgtggaccgt
     3001 gtacgcccat cgttagcgtc atctcgatat cggccgttcg cgaaggcccg ctgatcaaca
     3061 ccaaattgga actcccggcc acgtagggcg agccgacggc ccgcagccac ccctccaaat
     3121 cagcatagat gtctttcgat cgcacgaccg ctaagtgcgc ttcgggcagc agcgagggca
     3181 gcagcgaccg ccgcgatcct cccgagagaa tcagcgaccc ggtgtctgcg agaccggcca
     3241 cggccccggt caacccaacc gggaactcac caaatgcacc gatcgcccgc gtcccggcgg
     3301 cgccctccgg tacctccggc tgaacgac
//
LOCUS       DATDPQ010000234         2544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_625374, whole genome shotgun sequence.
ACCESSION   DATDPQ010000234 DATDPQ010000000
VERSION     DATDPQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625374"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1123
                     /gene="selD"
                     /locus_tag="VI520_05260"
     CDS             56..1123
                     /gene="selD"
                     /locus_tag="VI520_05260"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:116..118,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HLF37323.1"
                     /translation="MSDQEVVKPFRLTALSSCAGUASKLPAETLAQVLRPIQEVFGAD
                     PPPDLLVGLAAPDDAAVWRIDEARLLVVTTDFFTPIVDDPYAFGAIAAANALSDLYAM
                     GATPFLALNIAAMPVDLDPAIIREILRGGAEKVKEAGAVIGGGHSVQDREPKYGLVAL
                     GMAESASLFTKDRAHPGDALVLTKPLGTGVTTTALIRDRGSAVHSAEAIDWMVQLNEP
                     AARLAKELGVRAVTDITGFGLLGHGLEMAEASRLGLSIELGEVPFFSGAREYAELGTF
                     PAGSRDNARYFGDRVRFDVQISTADRMLLFDAQTSGGLLLAVSAPKLDAFLTRAERIH
                     QPAWVIGRAQPGSGIEVRGGR"
     gene            1162..2085
                     /gene="mdh"
                     /locus_tag="VI520_05265"
     CDS             1162..2085
                     /gene="mdh"
                     /locus_tag="VI520_05265"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HLF37324.1"
                     /translation="MPKVTIVGAGQTGATTAHWLAERELADLVLVDIVEGMPQGKALD
                     LSQAMPVVESDVGVVGSNDYAATAGSDIIVITAGLPRKPGMSRDDLLEANRAIVRDAV
                     QGTLPLSPQAILIVLTNPLDAMAYLAMKVGGLTRERVIGQAGILDSARMRSFVARELV
                     TSVRNVHCYVLGGHGDDMVPLVRHSNVAGVPLDRILSPARLSAIVERTRKGGGEIVGL
                     LKTGSAFYAPSAALAQMVEAILRDRHLVVPASVYLEGEYGQRGIFFGVPVQLGAKGLE
                     KVIEYELTDTERQALEGSAGRVRETIEALKL"
     gene            complement(2099..>2544)
                     /locus_tag="VI520_05270"
     CDS             complement(2099..>2544)
                     /locus_tag="VI520_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37325.1"
                     /translation="PLCERLTVGGATPTPTLPPPYPAANLLLPRDGEGFDLASDTVSL
                     QWAGVATLREGEFYRITVVDVTETILGSGNKSLIDYVTDTKYIIPTSFRPAGSTPHVM
                     RWWIDTVRLTGTSPAGEARYVSAGASSLQRVFTWSGAAIPATATP"
BASE COUNT          427 a          742 c          910 g          465 t
ORIGIN      
        1 cgttggcacg cttcgaatgg gcgctttaca atctctgggt ccgaagaggt gtgagatgag
       61 cgaccaggaa gtggtcaagc cttttcgctt gaccgccctg tcctcctgcg cgggttgagc
      121 gtctaagcta cccgcggaga cgctggcgca ggttctgcga cccatccagg aagtgttcgg
      181 cgccgatcca cctcccgatc tgttggtcgg gctggcggct cccgatgatg ccgccgtctg
      241 gcggatcgac gaggcgcgcc tgctggttgt gacgacggac ttctttactc cgatcgtgga
      301 tgacccttac gcctttggag cgatcgcggc ggccaacgcg ctctccgact tgtatgccat
      361 gggagccaca cccttcctgg cgctcaatat cgcggcgatg ccggtcgatc tggatccggc
      421 gatcattcgg gagatcctgc ggggtggcgc cgaaaaggtg aaggaagccg gggcggtgat
      481 cggcggaggg cactcggtcc aagaccggga gccgaagtat gggctggtcg cgctgggaat
      541 ggccgagtcg gcttcgctgt tcaccaaaga tcgggcccat cccggcgatg cgctggtgct
      601 caccaaaccg ctgggcaccg gggtcaccac tacggcgctg atccgggatc ggggatcggc
      661 ggtgcactcg gcggaagcga tcgactggat ggttcaattg aacgagcccg ccgcacggct
      721 ggcgaaggag cttggggtgc gggcggtcac cgacattacc gggttcggcc tgctcggcca
      781 cggactggaa atggcagagg cctcccggct gggcctttcg atcgagcttg gggaagtgcc
      841 gttcttctcc ggcgctcggg agtatgccga gttggggact tttcccgccg ggagccggga
      901 caatgcccgc tactttgggg atcgggtgcg gttcgacgtg cagattagca ccgcagatcg
      961 aatgctgctc tttgacgccc aaacttcggg aggattgtta ctggcggtct cggcgccgaa
     1021 gttggatgct tttctcactc gagccgagcg gatccatcaa ccggcctggg tgatcggcag
     1081 ggcccaaccg ggtagcggca tcgaggtcag gggcggacgc tgaaatccgt ccgaagcaat
     1141 gcgaaatcaa cgggaggtag catgccgaaa gtaacgatcg tcggggccgg tcagaccggc
     1201 gccaccaccg cccactggtt ggccgagcgc gagctggcgg atctggtgct ggtggacatt
     1261 gtagagggta tgccgcaagg caaggcgctc gatctgagtc aggcaatgcc ggtagtcgag
     1321 tcggacgtcg gggtggtggg gagcaacgat tacgcggcga ctgccgggtc cgacatcatc
     1381 gtgatcacag ccgggctgcc ccgcaagccg ggcatgagcc gagacgattt gctggaggcc
     1441 aaccgcgcga tcgtacgcga cgcggtgcaa ggcaccttgc cgctatcgcc gcaggccatc
     1501 ctgatcgtac tgaccaaccc gctggacgcc atggcttacc tggcgatgaa ggtcggtggg
     1561 ctcactcgcg agcgagtgat cggccaggcc gggatcctgg attcggcccg catgcgttcc
     1621 tttgtcgccc gggagctggt tacgagcgtc cgaaacgtgc attgttacgt tctgggtggc
     1681 cacggcgacg acatggttcc gctcgtccgg cactccaatg tggccggggt accactggac
     1741 cggatcctga gtccggcccg gttgagcgcg atcgtcgagc ggacccgcaa gggaggcggg
     1801 gagatcgtcg ggctgctgaa gaccggcagc gcgttctatg ccccttcggc cgcgctggcc
     1861 cagatggtcg aagctattct gcgcgaccga cacctggtcg ttccggcttc ggtctacctg
     1921 gagggagagt acggacagcg tggaatcttc tttggggttc cggtgcagtt gggcgccaag
     1981 gggctggaga aggtgattga gtacgagctc accgacacgg agcgtcaagc gttggaggga
     2041 tccgccgggc gggtgcggga gacaatcgag gcgctcaagc tgtagcgcac ggagctggct
     2101 acggggtcgc ggtcgccggt attgccgcgc cagaccaggt gaacacccgc tggaggctgg
     2161 aggcgccggc tgaaacgtag cgggcttcgc cggcagggga ggttccggtg aggcgcaccg
     2221 tgtcaatcca ccagcgcatg acatgggggg tggagcccgc cggccggaag ctggtcggga
     2281 tgatgtactt ggtgtcggtc acatagtcga tcaggctctt gttccctgac cccaggattg
     2341 tctccgtcac atcgaccacc gtgatccgat agaattcgcc ctcccgcagg gtggccactc
     2401 cggcccactg taagctgacg gtatcgctcg ccaggtcaaa accttcgccg tcccgcggca
     2461 ggagcaggtt ggcggccggg tacggtggcg gcagagtggg cgtcggggtt gcgcccccga
     2521 ccgtgagtcg ttcgcagagc ggga
//
LOCUS       DATDPQ010000235         5482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2736222, whole genome shotgun sequence.
ACCESSION   DATDPQ010000235 DATDPQ010000000
VERSION     DATDPQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736222"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1187)
                     /locus_tag="VI520_05275"
     CDS             complement(252..1187)
                     /locus_tag="VI520_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37326.1"
                     /translation="MRGTPFLLVAAAGILVSACIFEWQVTPTRPVESQSSIPATSAPT
                     QEPTRSPRPTASASPLPTPTRAEFVPEPDFFERSPDGQWVANSFGYYPPIQLQVARAD
                     GSVAWEVESDGDGWSEVILRPAHWSMDGRYLYFTLAPFVDGFLLYMDGSGLQRLDLEN
                     GQVMEVLAGEGELQCFSFSPDSTQLAYVRSEVDSERLIVRDLATEEELQWKLAAVPVQ
                     AGNITWSPDSTGLVLLVTRGFSEGEARTAVVLVTLSSAVPKTVLQEQPRIFYRILWVD
                     EHTVYLEDLTVTGWKLDLQTGEMALTVTPVPTPVP"
     gene            complement(1184..2023)
                     /locus_tag="VI520_05280"
     CDS             complement(1184..2023)
                     /locus_tag="VI520_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HLF37327.1"
                     /translation="MFRLTSDPGIDRDPTWAPDASRIAFTSDRTGTFEIYSLNVDGTG
                     LAQLTDDGEFGLDKQTPDWSPDGKRIAFVTLFDLYFIYAFKVEPAYSQPFSALDDSRK
                     SLLSDLSTDSMSPAWSPDGSLIAFTMKDMSLVSQVFLLDPVTWSSLQLTHGTYPAYGP
                     KWSPDGRQIAFTSVPSGHLDIYVIGVDGTGLTRLTDYPANDGDVSWSPDGRYIAFDSD
                     RGGCCALYIMRADGSEVVRLDVGEGDNFDPVWSPDGQYIVFVTDRDGNLEIYRIDAPT
                     LQP"
     gene            complement(2236..3702)
                     /locus_tag="VI520_05285"
     CDS             complement(2236..3702)
                     /locus_tag="VI520_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37328.1"
                     /translation="MTEIYVDTDQMRWQITLLRVFLDSVRGAQRDIFEATQIATGYEG
                     QLQRQVIAIAGNDESLVSTLANELEEMIYRVTQITDAFEAADAIGASNPTLGADFTRL
                     IDGGLLLSSLPSTYRYWDPLLELEERLWLTHDPDERALLPAGGINGHLRAFAQSSLFP
                     GNGSEADYWRALQVFLYVSGAIDTQPPLDTWQAAAQASGVELDAYVSTATGVKEEHVD
                     AWFRAAETHAVGLKTAIGEIVDMAQAGESIEQHFGFGMEGNWTESDKDAMAEGVLMVS
                     YALAGATPERESAADLFKAVFGGDVQFLLKGSGETETWWCQRAGFGFQCEPLARGDMS
                     PQLTAHELAHAFNATIVNNLNEGLSSVRPDLREHIENQIVEISPYGELADTRIVVEID
                     GGVVHVSGRPPGGGYERTDLGFASQGAPWQQHSIRRDGGNAAGEDFADMFLGWTFLGF
                     SDDPAGQARYAWMEQHMPEWIPQAAGPLDLSWFEIPTP"
     gene            complement(3766..4095)
                     /locus_tag="VI520_05290"
     CDS             complement(3766..4095)
                     /locus_tag="VI520_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37329.1"
                     /translation="MPPTFPEVYRWETRTIHPAEAAEIGGKIRLAGMSLESNGYHLGN
                     VKSKLEQCWKGQAKDRFFEDYGFARVPRRVEDLGLSIQDHGEQITRITVTVTEQVPIE
                     TDPTREI"
     gene            complement(4487..5251)
                     /locus_tag="VI520_05295"
     CDS             complement(4487..5251)
                     /locus_tag="VI520_05295"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37330.1"
                     /translation="MISDAVLRAVDLGMVFQNGNGGLQALERVSFETARDSFVCVVGP
                     SGCGKSTLLRLLGGLLRPTAGEVQFEGLRLEGPRRRIGFVFQQATLMPWRTAIENVAL
                     PLEVQWINSNELSERSGSLLDLVGLRGFEASLPRDLSGGMAQRVAIARALVHRPDLLL
                     LDEPFGSLDALTRERMAFELMRIWRAQTVSVVMVTHSIPEAVLLADRVLVLSPRPGTI
                     ALDLPIPLPRPRKLEMTHTPEFGALALQIRSAIGES"
     gene            complement(5248..>5482)
                     /locus_tag="VI520_05300"
     CDS             complement(5248..>5482)
                     /locus_tag="VI520_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37331.1"
                     /translation="AAFEICKKYVEGLDQLSAEDQALQRQILDASIEFWRADRLGYSD
                     PQAWQNMQEVLLAMGLLAEPLELAEAFSNEYLP"
BASE COUNT         1067 a         1729 c         1694 g          992 t
ORIGIN      
        1 gatgccgcga tctgggtgtt gatctgtgtg ctgccgattg gtctgatcct gggggtcccc
       61 ggctacttcg ccgggcggtc gatccgccgg cggcgccagg ccagagggaa gaaaacggcc
      121 tcggccaaag agtaagcgca accactccga gatagtgaaa gggacggtcc agagtgggcc
      181 gtccctttgc ttttccgctg tttgcgccgc ctcgcagcgc gcccagttcg agcggcggca
      241 tatgagtcca gtcagggtac cggcgtgggc accggagtga cagtgagggc catctcgccg
      301 gtttggaggt cgagtttcca gccggtgaca gtcaagtctt ccaggtatac cgtgtgctcg
      361 tcgacccaaa gaatccggta gaagatgcgc ggctgctcct gcaggacggt cttcgggacc
      421 gcgctgctca gagtgacgag cacgaccgcg gtgcgggcct ccccttcgct gaagccccgc
      481 gtgactagca gcaccagccc agtggaatcc ggcgaccagg tgatattccc cgcctggacg
      541 ggtaccgcgg ccagcttcca ctggagttcc tcctcagtgg ccaagtcccg cacaatgagc
      601 cgttcagaat ccacttcgct gcggacatag gcgagttggg tcgagtcggg ggaaaacgag
      661 aaacactgca actctccctc gcctgcgagc acttccatta cctggccgtt ctccaggtcc
      721 aggcgctgca gcccggatcc gtccatatac aggaggaaac catccacgaa cggagccaag
      781 gtgaagtaca ggtagcggcc gtccatggac caatgggccg gcctcaggat gacctccgac
      841 catccatccc cgtcggactc cacctcccag gcgaccgacc catcggcccg tgccacctgc
      901 agctggatcg gggggtaata accgaaagag ttggcgaccc actggccatc gggcgagcgc
      961 tcgaagaagt cgggctcggg gacaaactcg gcccgagtgg gtgtcggcag gggcgacgcg
     1021 gaggcggtcg gccgtggtga gcgcgtaggc tcttgcgttg gagcggaggt ggccgggatg
     1081 ctactctgtg attcgaccgg ccgggtgggg gtaacctgcc attcgaagat gcacgccgag
     1141 accaagatcc ccgctgcggc gaccagtagg aaaggggttc ccctcaaggc tgaagcgtcg
     1201 gcgcgtcgat gcggtagatc tcgagattcc cgtcccgatc cgtgacgaaa acaatgtact
     1261 gcccatccgg cgaccagacc gggtcgaagt tgtcgccctc gccgacatcc agccgcacca
     1321 cttcggatcc gtcggctcgc atgatgtata gtgcgcagca accaccccgg tccgagtcga
     1381 aggcaatgta ccggccatca ggggaccagc ttacgtcacc atcgttggct ggataatcgg
     1441 tgaggcgcgt caaacctgtg ccatcgacgc cgataacata aatgtccaga tgaccggaag
     1501 gcaccgaggt gaaggcgatc tgcctgccgt cgggagacca tttcgggcca taggccgggt
     1561 acgtgccgtg cgtcagctgc agggaggacc acgtcaccgg gtccaaaagg aacacctgcg
     1621 aaacgagcga catgtccttc atggtgaagg caatcaggct cccatcggga gaccaggccg
     1681 ggctcatgct gtcggtggac aggtcgctca gcagcgactt gcgggagtcg tccaaggcgc
     1741 tgaaaggctg agagtaggcc ggttccacct tgaaggcata gatgaaatac aaatcgaaaa
     1801 gcgtcacaaa ggcgatccgc ttgccatccg gtgaccagtc gggcgtctgt ttgtcgaggc
     1861 cgaactctcc gtcgtctgtc agttgggcca agccggtacc gtccacgttg agggagtaga
     1921 tctcgaaggt ccccgtacgg tccgacgtga aggcgatccg gctcgcgtcg ggagcccacg
     1981 ttggatcccg gtcgattccc ggatcgctgg taagccggaa caaccccgtc gccgcaatga
     2041 ccgggacttc gctcgcagag gtcgcagtcg gggtcgagga agcctcgacc gtcgccgtcg
     2101 ctgtgggact taaggttggc gaaggctctg ccggctgcgg ggtatcggtc ggtgcgactg
     2161 ccgtcggagc gcggcacgcc gcagccagca aaagaagccc tgcggcggaa actgctctcg
     2221 cagggagcac attcctcatg gggtcggaat ttcgaaccag gacaagtcaa gcgggccagc
     2281 ggcctgcggg atccactccg gcatgtgctg ctccatccag gcatagcgag cctgcccggc
     2341 cgggtcgtcg ctaaagccga ggaatgtcca gcccaggaac atgtcagcga agtcctcgcc
     2401 ggccgcgttg ccgccgtccc tccggatgga gtgctgctgc cagggtgccc cttgcgacgc
     2461 aaagcctagg tcggtgcgct cgtagccgcc cccgggcggc cggccactca cgtgaaccac
     2521 tcccccatcg atttccacca cgatgcgggt gtcagcgagc tctccatacg ggctgatctc
     2581 aacgatctga ttttcgatgt gctctctcag atcagggcga acgctactta gcccctcatt
     2641 gagattgttg acaatggtcg cgttgaaggc gtgtgccagc tcgtgagcgg ttaactgagg
     2701 cgacatgtcg cctctcgcga gcggctcgca ctggaagcca aaacccgctc gctgacacca
     2761 ccaggtctcg gtttctccgc tgcccttcaa cagaaactgg acatcgccgc caaagacagc
     2821 cttgaaaaga tcagccgctg attctcgctc gggcgtcgcc ccggccaggg cgtaagagac
     2881 cattaggacc ccctctgcca tggcatcctt gtccgactcg gtccagtttc cctccatacc
     2941 gaaaccgaaa tgctgctcga tgctctcccc cgcctgggcc atatcgacga tttcgccgat
     3001 cgccgttttg agccccacgg cgtgggtttc tgctgcccgg aaccaggcat ccacgtgctc
     3061 ttccttgaca ccggtggcgg tggagacgta tgcatcgagc tccaccccag aggcctgggc
     3121 ggccgcctgc caggtgtcca gagggggctg ggtgtcgatg gctccgctca catacaggaa
     3181 cacctgcagg gctctccagt agtccgcttc ggagccgttg ccgggaaaca gcgaggattg
     3241 agcaaaggcg cgcaaatgcc cgttgatccc gccggccggc agcaatgcgc gctcatcggg
     3301 gtcgtgggtc aaccagagcc tctcctctag ctcaaggaga gggtcccagt acctgtacgt
     3361 cgatggaagg gaggacagca gcagtccgcc gtcgatcagc cttgtgaaat cggcaccaag
     3421 cgtaggattc gaggctccta tggcgtcggc ggcctcgaag gcgtcagtga tctgtgtcac
     3481 gcggtagatc atttcctcca gctcgttggc cagggtgctc accaggctct cgtcgtttcc
     3541 ggcaatcgct atcacctgtc gctgcagctg gccttcataa ccggtagcta tctgggtggc
     3601 ttcgaagatg tctctctgcg cgccacgaac cgaatcgagg aacacacgaa ggagcgtgat
     3661 ctgccacctc atctgatcgg tgtcgacgta gatctcggtc atggacactc tcgggagaga
     3721 ctcacgaggc agggttcggt acttgagtgc agaggggtat cagggctata tctcgcgggt
     3781 agggtcggtc tcgatgggca cttgttcggt gacggtcact gtgattctgg taatctgctc
     3841 gccgtgatcc tgaatggaca ggcccaggtc ttccacgcgt ctcggtacgc gggcgaagcc
     3901 gtagtcctcg aaaaagcggt ctttggcctg ccctttccag cactgctcga gcttgctctt
     3961 cacattcccg agatggtatc cattgctctc caggctcatc ccggcgaggc ggatctttcc
     4021 cccgatctcg gcagcctcgg cgggatgaat agttctcgtt tcccatcgat acacttcagg
     4081 gaaagtgggg ggcacggcca tactcctata gcgagtcagc agcagtgtat gggcaagccg
     4141 cggcatagtc aatgtgcgcg ctggctcacg gggtcaagcg agtggcgagg gaccaagctg
     4201 gatcgcgcgc cgtttcatgg ccgcgaaccc cggacggcgg aggcatgtca ccagtcacgg
     4261 ccgcaggtcc caagcttcga gcctcgacct attaccttat acctagtccc tgttcaacga
     4321 tgtgtgatgg ctgatagcaa gcaactgata gccgatgggg ctagaagccg ccgtgagggc
     4381 cgtctcgaaa gcagcctgag acaaccatac tcttgaccga gggtgaaacc gaatcgcgcg
     4441 ggagattgcc ccctacgcag ccaatagcaa ccagagcggc ctgcattcag ctttcgccga
     4501 tcgccgaccg aatctggaga gccagtgcgc caaactcggg agtgtgggtc atctccagtt
     4561 tgcgcggccg gggcagcggg atcggcaggt cgagcgcgat cgttccgggc cgcggactga
     4621 gcaccagcac ccggtcggcc agcaacaccg cctccggaat ggagtgggtc accatgacca
     4681 ctgataccgt ctgggcccgc cagatcctca tcagctcgaa ggccatccgc tcgcgagtca
     4741 gcgcatcgag cgaaccgaac ggctcatcca gcagcagcag gtcgggtcga tgaaccagcg
     4801 cccgggcgat cgcgacccgc tgggccattc ctcccgaaag gtcccgcggc agactggcct
     4861 caaagcctct cagtccaacc agatccagga gcgaacccga tcgctcggac agctcattgg
     4921 aattgatcca ctgcacttcg agcggcaagg cgacattctc gatagcggtc cgccagggca
     4981 tcagagtagc ctgctgaaac acaaaaccaa ttcttcgccg cgggccttcc agccgcaagc
     5041 cctcgaattg gacttcgccc gcggttggcc gcagcagccc gccaagcagc cgcagcaggg
     5101 tgctcttccc gcagccggag ggtccaacca cacagacgaa ggaatcccgc gccgtttcga
     5161 aggacacccg ctcgagggcc tgcagccccc cgttcccgtt ttggaagacc atgccgagat
     5221 cgacagcccg cagtactgcg tcgctgatca aggcaggtac tcgttgctga aggcctcggc
     5281 caactcgagc ggctcagcga gcaaccccat cgccagcagg acctcctgca tgttctgcca
     5341 ggcttgcgga tcggaataac ccaaccgatc ggctctccag aactcgatcg aggcatccag
     5401 aatttggcgc tgcagcgcct ggtcctccgc gctgagctga tccagccctt cgacgtattt
     5461 cttgcaaatc tcgaaggccg cc
//
LOCUS       DATDPQ010000236         4780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2217471, whole genome shotgun sequence.
ACCESSION   DATDPQ010000236 DATDPQ010000000
VERSION     DATDPQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217471"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VI520_05305"
     CDS             <1..392
                     /locus_tag="VI520_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HLF37332.1"
                     /translation="TFILIVSSLTMVFGLAAIESGDQRRLRQFLVATAVLGSIFLSIQ
                     AVEWRELLAEGTTASTSLFGSTFFTLTGFHGLHVLGGVLFMFYVIARAFQGRYTKEEH
                     GGVELMGLYWHFVDVVWIFLFTIVYLV"
     gene            411..686
                     /locus_tag="VI520_05310"
     CDS             411..686
                     /locus_tag="VI520_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HLF37333.1"
                     /translation="MSAAKRSRYLTVFAALAVFTVIEVAVAFTAITKSTQVVLLLGLA
                     LIKASLVALYYMHLRSEGPLLRVIAAFPMLLVIVLLLLPVFDLALAQ"
     gene            727..2553
                     /locus_tag="VI520_05315"
     CDS             727..2553
                     /locus_tag="VI520_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLF37334.1"
                     /translation="MSEPEATVVANESVELRPWAAGPVYKKALIPTDNSELCHAGIDW
                     GVDFARKWGTLLVGNHVYAARLHDDRFRQLEVGLPARFQTEKEIGRQRNIHDKLIETG
                     LILISDSFLDVLDKRGEENDVQIERKLMEGIHFEQLSKDINSNDYDLVIMGAHGIGQV
                     KRSQLGMVTARVSRLITTDMLIMRQSGRKLSGGKILVCVDGSAYCFVALRKALRLSQE
                     FNTKVHCVSAFDPFYHYAAFDNIRHVLSYQAGKVFKFEEQEELHNNIIDRGLKKVCEA
                     NLLRAEKVAAEEGVEITSEVLVGKPFAKIVDVCERDDYDLVVVGRWGAHHVEGADLGS
                     NTDNLLRLAINNLLIVGAADARPFDIPMLEEYEEEPLDWDDDALKLVLRAPPFAQAMA
                     KASVEEYARHEGYERVTIDLLYGALSGILPPSAKHLMGLETEDRKLAEARQKARSPIE
                     EYVPHERAFPWTEAALQRVQSRVPPFIQPMAMLAMERYAREKQLPEVTLEVTIQVARR
                     LGYAGDDGPGSELVWNEAALERLSRVPKFERQMVREMVDSMAREAGHQEVNYEIADEM
                     LRRVRELWEAEGEGAFGYFKGMSGPQSVDQAPGSGEASASRD"
     gene            2593..3429
                     /locus_tag="VI520_05320"
     CDS             2593..3429
                     /locus_tag="VI520_05320"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HLF37335.1"
                     /translation="MQNLPAVDVLGQVVAQRTAVARRLASVRRVVAIAGGKGGVGKSL
                     LTANLAAALATDAARVGVLDADIHGPSAALMLGARGQRPKLGPQGARPAIGVCDVRVM
                     SMDLLLNDDDSAVRWRHYGGLADDSFVWRGTLEANVLREFLADTEWGELEYLLIDMPP
                     GADRFDTLLRLLPELSGAAMVTTPSEASVLVVRRAVAAAQHSGARILGLVENMAGGSG
                     PGRGRDLADDLGIPFLASVPFDPDLARNTEAGRPGVLVDPGAPGARAILELADRLRGI
                     LA"
     gene            3459..3869
                     /locus_tag="VI520_05325"
     CDS             3459..3869
                     /locus_tag="VI520_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37336.1"
                     /translation="MEFVSNDTDRASGVMAIQYSCPNCGRRISMVTNPGETQMVSSLG
                     VTIGHETLAEGGAGPMKQIRRSLEGEASWTEDRGSPDPVWTEAAEKRLAAAPGFVQGM
                     VRRLYTDWAKRNGFEEVSPAVMSKARDALGMSEM"
     gene            3866..>4780
                     /locus_tag="VI520_05330"
     CDS             3866..>4780
                     /locus_tag="VI520_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLF37337.1"
                     /translation="MIARPGRPQRQAGTSTQATFRPSLVSWNLTKRCNLRCPHCYLEA
                     GVKSEHELSTTECLGLIDEMAGLGTEMLILTGGEPLLRKDIYDIASYASQRGIWVVMG
                     TNGVLVNDLVAQKMIECGVRGVGISIDSTDPSVHNSFRGGPNSWEQSVRALEICRAHG
                     LEVLVQSTVMDLNYSEVPRLLEFARQLGAWSFNLYFLVQTGRGQQMNDLTAERTEQML
                     TFLAEAQQRYRPMLVRAKCAPHFKRIAFEAGLGGMESGGCMAGTEYCRITPDGEVTPC
                     PYMALPVGNVRGSSLTQIWQSAPQLLALR"
BASE COUNT          894 a         1392 c         1568 g          926 t
ORIGIN      
        1 acaccttcat cctgattgtg agcagcttga ccatggtgtt tgggttggct gcgattgagt
       61 ccggcgacca acgtcgcctg cgtcaattct tggtcgccac cgcggtcctg ggcagcatct
      121 ttttaagcat ccaggcagtc gagtggagag aattgttggc cgagggcacg accgccagta
      181 ccagcctctt cggatcgacc ttcttcaccc tcaccgggtt ccacgggctg catgtcttgg
      241 gaggggtgct gttcatgttc tacgtgatcg cccgtgcctt ccagggccgc tacaccaaag
      301 aggaacacgg cggggtggaa ttgatgggtt tgtactggca cttcgtggat gtggtatgga
      361 ttttcctgtt cacaattgtt tacctggtct agccggagag tagactctct atgagtgcag
      421 cgaagaggtc aaggtatctg acggtttttg ccgctctggc ggtgttcacg gtaatcgaag
      481 tggcggttgc cttcaccgcg attacaaagt cgacccaagt cgtcttgctg ctcgggctgg
      541 cgctgatcaa ggcaagcctg gtggccttgt actacatgca tctgcgctcc gaaggacccc
      601 tgctaagggt catcgccgcg tttccgatgc tgctggtgat cgtgctgttg ttgctgcccg
      661 ttttcgattt ggccctggcc caatagccgg ccagccgcgc aagaatctca tcgtacaggg
      721 tgatcaatga gcgaacctga agcgaccgtc gtagcgaacg aatccgttga gctacgcccg
      781 tgggcggcgg gcccggtcta caagaaggcc ctgatcccaa ccgacaattc cgagctctgt
      841 catgccggga tcgactgggg ggtggacttt gcccgaaagt gggggacctt gctggtcggc
      901 aaccatgtct atgccgcccg gttgcatgac gaccgtttcc gtcagctgga agttggcctg
      961 ccggcccgct tccagacgga gaaggaaatc gggcgtcagc ggaatatcca cgacaagctg
     1021 atcgagaccg ggctgatcct gatctcggat tcgttcttgg atgtgctgga caaacgcggc
     1081 gaggagaacg acgtccagat cgagcgcaag ttgatggagg gcattcactt cgaacagctc
     1141 tcaaaggaca tcaactccaa cgattacgat ctggtcatta tgggtgccca cggcatcggt
     1201 caggtcaaac gcagccagct cgggatggtg accgccaggg tctcgcgcct gatcacgacc
     1261 gacatgctga tcatgcgtca atccggccgg aagctgagcg gcgggaaaat cctggtgtgc
     1321 gtcgatggtt cggcctattg cttcgttgcg ctccgcaagg ccctccggct gtcccaggaa
     1381 ttcaacacca aggttcattg cgtctcggcc ttcgatccct tctatcacta cgcggccttc
     1441 gataacattc gccatgtact ttcctaccag gccggcaagg tgttcaagtt tgaagaacag
     1501 gaagaactcc ataacaacat catcgaccgc ggcctgaaga aggtttgcga agccaacctg
     1561 ctccgggccg agaaagtggc ggccgaggag ggagtggaga tcacctccga agtcctggtt
     1621 gggaagccgt ttgcaaagat cgttgatgtc tgtgagcggg acgactacga cctggtcgtg
     1681 gtcggccgtt ggggggccca ccatgtcgag ggcgcggatc tcggctccaa caccgacaat
     1741 ctgctccggc tggcgatcaa caacctgctg atcgttgggg cggccgatgc ccggcccttc
     1801 gatatcccga tgctcgaaga atacgaggag gagccattag attgggacga cgacgccctc
     1861 aagcttgtac tgcgggcgcc gccctttgcc caggcgatgg ccaaggcttc ggtcgaggag
     1921 tatgcccgcc acgagggata cgaacgagtg acgatcgatc tgttatacgg ggcgctcagc
     1981 gggatcctgc ctccgtctgc caagcatctg atggggctcg agaccgagga ccgcaaactc
     2041 gccgaggccc gccagaaagc ccgctcgccg attgaagaat atgtgccgca cgaacgagcc
     2101 ttcccctgga cagaagcggc cctgcaacgg gtgcagtccc gggtgccccc cttcattcag
     2161 ccgatggcca tgctggccat ggaacgctac gcccgagaga aacaacttcc cgaggtgact
     2221 ctcgaggtga ccattcaggt ggcgcgccgg ctgggttatg ccggagacga tgggccgggg
     2281 agcgagctcg tctggaatga agcggccctt gagcgtctgt cgcgagttcc taagttcgag
     2341 cgccagatgg tcagagagat ggtcgacagc atggcccgcg aagccggaca ccaggaagtc
     2401 aattacgaga ttgccgacga aatgctgcgc agggtgcgcg agctctggga ggccgagggc
     2461 gagggtgcct tcggctattt caagggtatg tccggcccgc agtccgtgga ccaggcccca
     2521 ggctccggtg aggcatctgc ctcacgcgat tgaagccgcg ggagtgaggc ccgcggcagt
     2581 gcgggattga gcatgcagaa tcttccggcc gtggacgtgt tggggcaagt ggttgcccag
     2641 cgaaccgctg ttgcccgccg gctcgcttcc gtccggcggg tcgtggccat cgccggcggc
     2701 aagggtgggg tcggcaagag tctgctgacc gccaacctgg ccgccgccct ggcgaccgac
     2761 gccgccaggg tcggggtgct ggacgccgac atccacgggc cgagcgcagc cctgatgctg
     2821 ggtgcccgcg ggcagcggcc aaaacttggc ccgcaggggg cgcggccggc cattggagtt
     2881 tgcgacgtgc gggtcatgtc catggactta ttgctgaacg atgacgattc agcggtccgc
     2941 tggcgacact atggggggct tgccgacgat tcgtttgtct ggcgcgggac actcgaagcc
     3001 aacgtcctgc gtgagtttct tgccgacacc gagtggggcg aattggagta tctgttgatt
     3061 gacatgcctc cgggtgcgga ccgtttcgat accctcctga gattactgcc ggaactcagt
     3121 ggcgcggcca tggtcactac cccaagcgaa gcctcggtgc tagtggtccg aagagcggtc
     3181 gccgcggctc agcactccgg ggctcgaatc ctcgggctgg tcgaaaatat ggccgggggc
     3241 tccggaccgg ggcgcggtcg ggacttggcc gatgacctgg ggatcccctt cttggccagt
     3301 gtcccgttcg atcccgacct cgcccggaac accgaggccg ggaggccggg ggtgctcgtt
     3361 gaccccgggg cgcccggagc acgggcgatc ctcgaactgg ccgatcggct cagaggaatc
     3421 ctggcgtgaa attccgctgc gaagagtgtc aggaaacgat ggagttcgtt tcgaatgaca
     3481 ccgaccgcgc cagcggcgtg atggctatcc agtattcctg cccaaattgc ggccgccgga
     3541 tttcaatggt caccaatccg ggcgagaccc agatggtgtc cagccttgga gtgaccatcg
     3601 gccacgaaac gctcgccgag ggcggggccg ggcccatgaa acaaatccgg cgctcgctag
     3661 aaggggaagc gagctggacc gaagaccggg gttcgccgga tccagtgtgg acggaagccg
     3721 ccgaaaaacg gttggctgcg gccccgggat tcgttcaggg gatggtccgc cggctgtaca
     3781 ccgattgggc aaagcgtaat gggtttgagg aggtcagccc ggccgtaatg agcaaggccc
     3841 gcgacgcgct cggaatgtcc gaaatgtgat tgcacgcccg ggaagacccc agaggcaggc
     3901 gggcacgagc acccaggcta cctttcggcc gtccttggtt tcatggaatc tgaccaagcg
     3961 ctgcaacctg cgctgccccc attgctacct ggaggctggg gttaagtccg agcatgaact
     4021 cagcactacc gagtgcctcg gcttgatcga cgagatggcc gggctgggga ccgagatgtt
     4081 gatcctgacc ggcggcgaac cattactgcg gaaggacatc tacgacatcg ccagctacgc
     4141 ctcccagcgc gggatctggg ttgtcatggg caccaatggc gtgctggtaa acgatttggt
     4201 cgcccagaaa atgatcgagt gtggggtgcg gggagtcggg atcagcatcg actcgaccga
     4261 tcctagcgtt cacaatagct tccggggtgg ccccaattct tgggagcaat cggttcgggc
     4321 gctcgaaatc tgccgggccc acgggcttga agtcctggtc caaagcactg tgatggatct
     4381 caactactcc gaggtgcccc gcttgctgga gttcgctcgc cagctcggag cctggtcctt
     4441 caatctctat ttcctggtcc agaccggccg tggtcagcaa atgaacgatc tcaccgccga
     4501 gcggaccgag cagatgctga cgttcctggc ggaggcccag cagcgctacc ggcccatgtt
     4561 ggtgcgtgcc aagtgcgctc cgcacttcaa gcggatcgcc ttcgaagccg gtttgggcgg
     4621 gatggagagc ggcggctgca tggcggggac cgaatactgc cggatcactc cggatggcga
     4681 ggtgactccc tgtccctaca tggctctgcc ggtgggaaat gtccgcggca gctcgttgac
     4741 ccagatctgg cagagcgcgc cgcagctgtt ggcgctgcgg
//
LOCUS       DATDPQ010000237         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1691599, whole genome shotgun sequence.
ACCESSION   DATDPQ010000237 DATDPQ010000000
VERSION     DATDPQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691599"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VI520_05335"
     CDS             <1..1524
                     /locus_tag="VI520_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF37338.1"
                     /translation="QELKRRNDPLALFLVDQRMPEMTGTEFLAQAIKLYPEARKVLLT
                     AYADTEAAIQSINSIGLDHYLMKPWEPPEHNLFPVLDDFLSDWQANVVLPFDGIRVAG
                     TLWSPGSHAVKDFLARNQVPYQWLNVETDLQARELVQTTDPEQHRLPVVFFPDGSYLV
                     EPNNLALAAKVGLQTEARQTFYDFIIVGGGPAGLGAAVYSASEGLRTAMIEREAPGGQ
                     AGTSSRIENYLGFPKGLSGADLARRASAQAKRFGAEIISAVEAEKVRTDGTYRYVTCS
                     GGAELSCRALLIATGVTVRMLEAPGAAELTGRGVYYGASLTEAAFYKDQPVVVVGGAN
                     SAGQGAMFFTEYASQVTMLLRGSSLESGMSQYLVDQIKGQPNITVRTGTTVTEVGGEG
                     RLEQVTIRDEPSGAIETLRAAAMFVFIGAVPHTDLVAGIVERNPAGFIVTGQDLFHNG
                     KRPSGWTLKRDPFLLETNVPGIFAAGDVRQGAVRRVASAVGEGAMVVSFVHQYLQTV"
     gene            1524..>2161
                     /locus_tag="VI520_05340"
     CDS             1524..>2161
                     /locus_tag="VI520_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLF37339.1"
                     /translation="MFDALRRSELFGELSDDDLQRLCLNIEYVRLAAGQTLFAQDSPG
                     DKAYFIQEGQLEILNTSGDREVLIAVRKPGELIGEMALLEQAPRSAAVRARQDSLLYA
                     IGKQHFDQLFGSSPAALKNMLHTVLFRLRETQGMLRHSERMAQLGTLTAGVAHELNNP
                     ASAVRRAADQLREELPRFKQAQLALQLLSLTAGQRNKLNAEIRQAEEQAGRPP"
BASE COUNT          413 a          676 c          689 g          383 t
ORIGIN      
        1 caggaactga agcgccgcaa cgaccctctg gcgctctttc tcgtcgacca acgcatgccg
       61 gagatgaccg gcaccgagtt cctcgctcag gccatcaagt tgtatcccga ggcgcgcaag
      121 gtcttgttga cggcctacgc agataccgag gccgctatcc agagcatcaa cagcatcggg
      181 ctcgaccact acttgatgaa accttgggag ccgccagagc acaatctgtt cccggtactg
      241 gatgacttcc tcagcgattg gcaggcgaat gtcgtcctgc cattcgacgg cattcgggtc
      301 gcgggaaccc tctggtcccc cggcagccat gccgtaaaag acttcctcgc ccgcaaccag
      361 gtcccctacc agtggctgaa tgtcgaaacc gacctgcagg cgagggagct ggttcagacg
      421 accgacccgg agcaacaccg gctgccggtg gttttcttcc cggacggcag ctatctggtc
      481 gagccgaaca acctagcttt ggcggcgaag gtggggctgc agactgaagc tcgccagacc
      541 ttctatgact tcattattgt gggtggcggc ccggccggtc tgggagccgc cgtctactcg
      601 gcctcggaag gcctgcgcac tgcaatgatc gaacgggaag cgccaggcgg ccaggccggg
      661 accagctccc gcattgagaa ttacctcgga ttcccgaagg gcctgagtgg ggcggacctc
      721 gcccggcggg ccagcgccca ggccaaacga tttggggccg aaatcatctc ggcggtcgag
      781 gccgagaagg tcaggaccga tgggacgtat cgctatgtga cctgttccgg tggagccgaa
      841 ctcagctgcc gggcgctgct cattgcgacc ggggtgaccg tccgcatgct ggaggcccca
      901 ggcgccgccg agctgaccgg ccgaggcgtc tactacggcg cctcgctcac cgaggccgcc
      961 ttctataaag accagccggt ggtcgtcgtg ggaggggcta actcggccgg ccagggggca
     1021 atgtttttca ccgagtacgc cagccaggtc accatgctgc ttcggggtag ctcgctggag
     1081 tcgggcatgt ctcagtatct ggtcgaccag atcaagggac aacccaacat cacggttcgg
     1141 acgggaacca ccgttacgga agtcggcgga gagggcaggc tcgaacaggt cacaattagg
     1201 gacgaaccat cgggagcaat cgagactctg agggcagccg cgatgttcgt tttcattggt
     1261 gcggtgccgc acaccgactt ggtggctggt atcgtggagc gcaaccccgc cggctttatc
     1321 gtcaccggac aggacctgtt tcataacggc aagcggccga gtggctggac cttgaaacgg
     1381 gacccgttcc tgctcgagac gaatgttccg gggattttcg cggccggaga tgtgcgccag
     1441 ggcgcggtcc ggcgggtcgc ctcggcggtt ggcgaggggg caatggtggt cagcttcgtc
     1501 caccaatatc tgcaaacggt ctgatgttcg acgcgctgcg cagatccgag ctgtttggcg
     1561 agctgtccga cgatgacctg cagcgtcttt gtctaaacat cgagtacgtt cgattggcgg
     1621 cagggcagac cctcttcgcc caggacagcc ctggcgacaa ggcttacttc attcaagaag
     1681 gacagctcga gattctgaat acctctgggg atcgggaggt gctgatcgcg gtccgcaagc
     1741 ccggcgaact gatcggggag atggcgctgc tggagcaggc accccgcagc gccgcggtac
     1801 gcgcccgcca ggatagtctg ctgtacgcca tcggcaagca gcatttcgac caacttttcg
     1861 gttccagccc cgcggccctg aagaacatgc tgcacaccgt cctcttccgg ctgcgcgaga
     1921 cccaaggcat gctccggcac agcgagcgga tggcccagct tggcactctc accgccggtg
     1981 tggcccatga gttgaacaac ccggcctccg ccgttcggcg ggccgcagat cagctgcggg
     2041 aggaactgcc tcggttcaag caagctcaac tcgcgctcca gctgctcagc ctcactgccg
     2101 gccaacgaaa taagctcaat gccgagattc gacaggctga agaacaggcc gggcgcccac
     2161 c
//
LOCUS       DATDPQ010000238         3309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_630837, whole genome shotgun sequence.
ACCESSION   DATDPQ010000238 DATDPQ010000000
VERSION     DATDPQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630837"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VI520_05345"
     CDS             complement(<1..288)
                     /locus_tag="VI520_05345"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLF37340.1"
                     /translation="MQRVDQAPAGLLRRQLEPTPANQLVVPRPEPAVRPAPPVPTRSP
                     RVISIVNRKGGVGKTTTAFNLAGALAGRGNSVLLIDLDPMGSLCRSLQIRPG"
     gene            complement(290..916)
                     /locus_tag="VI520_05350"
     CDS             complement(290..916)
                     /locus_tag="VI520_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37341.1"
                     /translation="MITTKRETTLAPVLLSDSAIGSPTIRVPRVLIVDDSPRFRLELR
                     GLLELEDIQVIGEAGNGWEALSLTETLNPDLILMDQNMPALSGIDATRRIKQARPDRR
                     VLFIAAEEAWRQEALKAGAEGYFVKGEGFEQLIQAIHQPGLGGALQWQIDSRRHRRVP
                     VRRVALTLGGLAVFALILVVFPLPTEWFPAIALILASFSLVLYLIGGD"
     gene            complement(1329..>3309)
                     /locus_tag="VI520_05355"
     CDS             complement(1329..>3309)
                     /locus_tag="VI520_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37342.1"
                     /translation="SSVARSGDWITTEYNNLSSPSTFYKIGPEQTATISPAGKTMNTI
                     EGSAGSTQTFNSVGSSYWYSDQHWPIGGDNATIAAGAYIGNLYFNSINSIDVTRVNDT
                     YTETADTVVTAHTPDIGTSWIEVFDNTTATTNATIIASLDIVQAGSTENAVGQAYTAQ
                     PNPTSTSQSVSFTLSAIDTTTGTKPVGLFARRTDNSNFYYLRILPNPHATINSITLWK
                     NVAGVLTQLATVDPTIAANDTFKLEVTDASKKVYHNGVEVLSSTDNALTGVGTWGFFI
                     SNFNGTGGNLSQAWDITSFLAEEPPSAIGPPQVATTNTSVNNTANTSHTVNLPSGIQS
                     GNLLIVIFGFDGGTNQTITWPIGWTSFFRSDRSTVVGVAAAYRKADGSEGSTITVQTS
                     ANTRSSHNSYRITGAADPATQAPESAASNGNSTTPNPPSLTPTGGAKNYLWIALETNN
                     DGTITITGYPANYSGGIQANGDLNASTTGSAARQLNAASEDPGTFTITGTGIAWAAGT
                     LVVHPAVPSVDITVRVHHTKADGTDPQLIINASTTVTSATADPLALALGSGALQTFTS
                     ADPRLLRLQIEVTAVKDGGSFVLDYDGTCATSKCSNLNTPVVTVPEYGIAFAAFVVLI
                     PVAMGGVWRRKRLIMRSRHAHDPSAHSRRGKAGRE"
BASE COUNT          560 a          942 c         1105 g          702 t
ORIGIN      
        1 gccaggccgg atttgcagcg accggcagag cgagcccatc gggtccagat cgatcaacag
       61 aaccgaattc cctctcccgg caagcgctcc ggccaggttg aaggcagtcg tggtcttgcc
      121 cacgcccccc ttgcggttca cgatagagat cacccgcggc gagcgggtcg ggaccggggg
      181 ggccggtctg accgcaggct ctggacgtgg aaccaccaat tggtttgccg gcgtcggctc
      241 aagctggcgc ctcagcaacc ctgcgggggc ttgatccact ctttgcatct cagtcacctc
      301 cgatcaggta gagcacgagc gagaaactcg ccagaatcag tgcgatagcc gggaaccact
      361 cagtcggcag cgggaatacg acgaggatga gtgcgaacac ggccaggccg cccaaggtca
      421 gggccactcg gcgtacgggg accctgcgat gccggcggct atcgatctgc cactgcaggg
      481 cgccgccgag ccccggttga tggatggcct ggataagctg ctcgaatcct tctcccttta
      541 caaaatagcc ctcggctccg gctttcaggg cctcctggcg ccaggcttcc tctgccgcaa
      601 tgaacagtac ccggcgatcg ggcctggctt gcttgattcg ccgggtcgcg tcgatgccgg
      661 agagggcggg catgttctga tccatcagga tcagatcggg gttcagggtc tcggtcaggc
      721 tcagcgcctc ccagccattg ccggcttcgc caattacctg gatgtcttcc aattcaagca
      781 acccgcgcag ctccaggcga aaacgcgggc tgtcgtcgac gatcaacact cggggcaccc
      841 ggatggtggg tgagccgatt gcactatcgg aaaggaggac aggtgccaat gtcgtctctc
      901 gcttagtagt gatcatgttc agctgcgatt ccggcagctg ggcgcaactg gaccatgggc
      961 agccctgtcc cgcatggctg ccaagagccg gccaccccag tcgcttctga cttggaactc
     1021 acgctcatca tagaacgtgg cccagtcgag tgagtcttca ttcaacttac agttcgctaa
     1081 agttcgtggc tctcgcatcg accttccttt tccgggttta gcctgtagct ccacctggaa
     1141 ttaaaaacgc cagtcagacg gttggccgtc cgctggcgtt tccactcgaa acgtactgtg
     1201 attatttcac gcgccgccct ctagggcatc cacaggccga actgaacctg ggaagtacaa
     1261 aggccgaacc ctggttcggc ctttagtctt agatttgggc ttgtcgggtc gggcttaggt
     1321 cttaggccct actctcgccc cgcctttcca cgtcggctat gcgcagaagg gtcgtgagcg
     1381 tggcgggacc gcatgatcag ccgctttctg cgccataccc cgcccatcgc gaccgggatg
     1441 agcaccacga aggccgcaaa ggcgatgccg tactcgggca cggtcaccac cggcgtgttc
     1501 aagttgctgc acttgctcgt tgcgcaagta ccgtcatagt caaggacgaa gctgccgcca
     1561 tctttcactg ccgtgacctc gatctgcagg cgaagcaggc ggggatccgc ggaggtgaag
     1621 gtctgcagtg cccccgagcc cagggcgagc gccagaggat ctgcggtcgc gctggtgacg
     1681 gtggtcgagg cgttgatgat cagctgcgga tcagtgccgt cggccttggt atggtggacc
     1741 cgaacggtga tgtcgaccga ggggaccgcc ggatgcacca ccaaggtgcc tgcggcccag
     1801 gcgatacccg ttccggttat tgtgaaggta cctgggtcct ccgaggcggc attgagctgg
     1861 cgagcggccg agccggtggt cgaggcgttg aggtcgccgt tggcctggat gccgcccgag
     1921 tagttggctg gatagccggt gatagtgatg gtcccgtcgt tgtttgtctc tagcgcgatc
     1981 catagatagt ttttcgcccc tccggttggg gtcaggctcg gcgggttggg ggtagttgag
     2041 ttgccgttgg aggcggcgct ctccggggcc tgcgtggccg gatctgccgc cccggtgatc
     2101 cggtagctgt tgtgcgagct ccttgtgtta gccgaagtct ggaccgtgat ggttgagcct
     2161 tcactgccat cggccttccg gtaggccgcc gcgacgccga cgacggtcga gcggtcggag
     2221 cggaagaacg aggtccagcc gatcggccag gtgatcgttt ggttggttcc accatcgaac
     2281 ccaaagatga cgatgagcag gttgccggac tggatcccgc tcggcaggtt caccgtgtgg
     2341 ctggtgttag cggtgttatt gaccgaggtg ttggtggtgg cgacttgggg cgggccaatg
     2401 gcgctcgggg gctcctcggc gaggaagctg gtgatgtccc acgcttggga caggttgccg
     2461 cctgtaccgt tgaagttgct gatgaagaac ccccaggttc caacgccggt gagcgcgttg
     2521 tcggtggaac ttagtacctc taccccgttg tggtacacct tcttggaggc atcggtgacc
     2581 tctagcttga aggtgtcatt cgcggcgatg gtcggatcta cggtggcgag ctgggttaat
     2641 accccggcga cgtttttcca gagcgtgatg ctgttgatgg tggcgtgagg gttaggcagg
     2701 atgcgcaaat agtagaaatt gctgttgtca gtccggcgag cgaagaggcc gacaggtttg
     2761 gtgccggtgg tggtgtcgat cgccgagagc gtgaagctaa cagactggga cgtgctggtg
     2821 ggattcggct gggccgtata cgcctgcccc acagcgttct cggtagatcc tgcctggacg
     2881 atgtccagcg aggcgataat ggtggcgttc gtggtcgcgg tcgtgttgtc gaagacctct
     2941 atccagctgg tgccgatgtc gggggtgtgc gccgtgacga ccgtgtcagc tgtctcggta
     3001 tatgtgtcgt tcacccgggt gacatcgatg ctgttgatgc tgttgaagta cagattcccg
     3061 atgtacgcgc cggcggcgat ggtggcgttg tcgccgccga tcggccagtg ctggtccgag
     3121 taccaataac tgctgcctac cgagttgaaa gtctgggttg agccggcgga gccctcgatc
     3181 gtgttcatcg tcttcccagc cgggctgatc gtggccgtct gttcagggcc gatcttgtag
     3241 aaggtggagg gcgagctcag attgttgtat tcggtagtga tccagtcccc ggagcgggcg
     3301 acgctcgag
//
LOCUS       DATDPQ010000239         3258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1179600, whole genome shotgun sequence.
ACCESSION   DATDPQ010000239 DATDPQ010000000
VERSION     DATDPQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179600"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="rsmA"
                     /locus_tag="VI520_05360"
     CDS             <1..880
                     /gene="rsmA"
                     /locus_tag="VI520_05360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLF37343.1"
                     /translation="SSAQLDRIYLPVVRPEFDARRLLKRHSVRPLKRLGQHFMIDPRA
                     LNQVVAAAELGGTETVLEVGAGLGSLTQALAIVARRVIAVELDPRLLPPLREVLHGLS
                     SVEILIGDILDLDLKSTIGEDPYLVVANIPYNITSTLIRHFLESLTAPHRMVLTIQRE
                     VAERVVAGPGQMSLLALGVRVYGNPTIRARVPARAFYPRPQVDSAVLRVDIHNQPTLD
                     GKLLDPVFRLAKAGFGQRRKMLRNTLATGLNWSVAEAADLLAGAGIAAQARPQELDVG
                     DWARLAGTALRLGLLD"
     gene            complement(877..1611)
                     /locus_tag="VI520_05365"
     CDS             complement(877..1611)
                     /locus_tag="VI520_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HLF37344.1"
                     /translation="MRPLTVVVPTYNEVSNLPSLIDALVDLSLPNLKILIVDDASPDG
                     TGRLADDLAGRMSGTLSVIHRSGKLGLGSAYLKGFQSAIQAGAQAIGQMDADFSHSPR
                     YLPEFLQRLESTDAVFGSRYVTGGRLDERWGIGRVFLSKFGNSYARVLLGLKVRDATG
                     GFRIWRAETLQAIPLGRISSEGYVFQVEMAYLAQRLGFEIAELPIYFEDRRIGQSKMS
                     LRIQIEAAVRVWLLPFIYRKLRPARS"
     gene            complement(1621..1965)
                     /locus_tag="VI520_05370"
     CDS             complement(1621..1965)
                     /locus_tag="VI520_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HLF37345.1"
                     /translation="MDRLRDLQSSSADVEASAVVSVDGLTMASALPASVEEDRVSAMS
                     AAMLSLGERIATELGRGKLDQVYIKGQTGYVILMSIEREAVLTVLARDRAKLGLLFLD
                     MRRAAEDLANLL"
     gene            2135..2779
                     /locus_tag="VI520_05375"
     CDS             2135..2779
                     /locus_tag="VI520_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLF37346.1"
                     /translation="MPAVLEQQIVAFLTELFQSIGWGGVLSIMALESANIPIPSEVTM
                     PLAGWLLVQANGGSGLAAIWLGGFWGGLGCTLGSVASYALGAFGGRGFLDRFGRYLMI
                     SHHDLEVADRWFARWGDWTAFLSRLLPIVRTFISFPAGVAKARFVPFVTYTFLGSFIW
                     CGALAYGGHAFGARWEELRAVMRPFDIPIGIVLMAGFAYYVVHHLRRGGTTHAG"
     gene            complement(2782..>3258)
                     /locus_tag="VI520_05380"
     CDS             complement(2782..>3258)
                     /locus_tag="VI520_05380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HLF37347.1"
                     /translation="IEESVKSVSIWPVIQRRPSVGQAFLGGVLAGSGYALFESLLLAQ
                     PGADWVSSMVARAGTPLIHAFGTGLVSWGLIEAIVNRRPLRLLALFLMAVGFHGIWNF
                     TALLVALSGFSLEIGAGVISPEGAKLVALSGLLVLLSLSAVALVGLVGLPRWLAKR"
BASE COUNT          626 a         1010 c          998 g          624 t
ORIGIN      
        1 ctcctccgcc cagctcgatc gtatatatct tcccgtagtg cggcccgaat tcgacgcacg
       61 ccggctgctc aagcggcatt cggtccggcc cctcaagcga ctcggccagc atttcatgat
      121 cgacccgcgg gcactcaacc aggtggttgc agcggccgaa ctcggcggga ctgagacggt
      181 gctggaagtc ggtgccgggc tcggttcgct gacccaggcc ctggcgatcg tagcccggcg
      241 ggtgatcgcg gttgagctcg acccacggtt gctgcccccc ttgcgggagg tgcttcacgg
      301 tctgagttcg gtcgagatcc tgatcggcga tatcctcgac ctggatctga aatcgaccat
      361 cggagaagac ccctacctgg ttgtggcgaa tatcccttac aacatcacct ccaccttgat
      421 ccgccacttc ctcgaatcac tgaccgctcc ccaccgcatg gtgctgacga ttcagcgcga
      481 agtagcggaa cgggtggtcg ccggtcctgg ccagatgagc ctgctggcgc tcggcgttcg
      541 tgtgtatgga aatcctacga ttcgggcccg ggtcccggcc agggccttct atcctcgacc
      601 ccaagtcgat tctgcggtgc tgcgggttga cattcacaac cagccgaccc tcgatgggaa
      661 actgctggat ccagtgttcc ggttggccaa ggcgggtttt gggcagcggc ggaagatgct
      721 caggaacact ctggcgacag gactgaactg gtcagtggca gaggcggccg atctgctggc
      781 tggggccggg atcgcggccc aagccagacc gcaagagctc gatgtgggag attgggcccg
      841 actggcgggc accgctctgc gactcggact gctggattag ctgcgggctg gtctcagctt
      901 ccgatagatg aacggtagca gccacactcg gacggccgcc tcaatctgaa tccgcagtga
      961 catcttggac tgcccgatcc tgcggtcttc gaagtagatc ggcagttcag caatctcaaa
     1021 gcccagtcgt tgggccaggt aggccatctc gacctgaaag acgtagccct ccgagctgat
     1081 ccggcccagc ggaatcgcct gcagggtttc cgcccgccag atgcgaaagc ctccggtcgc
     1141 gtccctgacc ttaagtccca agagcactcg ggcatagctg ttgccgaact tggaaaggaa
     1201 gactcgcccg atcccccatc gttcgtcgag ccgacccccc gtcacgtagc gcgaaccaaa
     1261 aacggcgtca gtactctcca accgctgtaa gaattccggc agatatctgg gcgaatggga
     1321 aaaatcggcg tccatctgtc cgatggcttg agcccccgcc tggatggccg actggaaacc
     1381 cttgaggtag gccgagccca ggccgagttt cccacttcga tggatcacag ataaagtgcc
     1441 ggacatccgg ccagccagat catccgccag tcgcccggtg ccatcgggcg aggcgtcatc
     1501 gacgatcagg atcttaaggt ttggaagcga gaggtcgacg agggcgtcga tcagcgaggg
     1561 gagattcgag acttcgttgt aagttgggac gacgacggtc agtggccgca tgcccgatcg
     1621 ctagagcaag ttggctaggt cctccgctgc ccgccgcata tccaggaaca gcagtcccaa
     1681 cttggctcga tcgcgggcga gaacggtcag caccgcctca cgctcgatcg acatcagaat
     1741 gacatagcca gtctggcctt tgatatacac ctgatccagt ttccctcggc cgagttcggt
     1801 ggcgatccgc tccccaagtg agagcatggc cgccgacatg gccgaaaccc gatcctcttc
     1861 cacactagcc ggcaaggcag aggccatcgt caggccatca acactcacca cggcggaggc
     1921 ctccacgtcc gcagaggagg actgtaggtc ccgcaggcgg tccatcatga gttcaagccg
     1981 aggtttagac acagtggggt gggatttgtg aacttatgtt caaaggcccg gtactgtagc
     2041 atacgggtta ggtcgagtca agcatgcatt gttcctaagc tcgatcgtgg atatactcgg
     2101 ccgagtgcag acctaaacgg aatcgacgga gcccatgccg gctgttcttg agcaacagat
     2161 cgtggccttc ctcaccgagc tgtttcagag catcgggtgg gggggagtcc tgtcaattat
     2221 ggcgctggaa agtgccaaca ttccgattcc tagcgaggtc acgatgccgc tggccgggtg
     2281 gttgctagtt caggccaatg gaggcagcgg gcttgcggcg atttggctgg gaggcttctg
     2341 gggtggactc ggatgcaccc tcggatcggt cgcttcctat gcgttggggg ccttcggggg
     2401 tcggggcttc ctcgaccgat tcggccgcta tctcatgatc agccaccacg acctcgaggt
     2461 cgcagaccgc tggttcgccc gctggggcga ttggacggct ttcctgtcac ggttgctgcc
     2521 gatcgtgcgg acgtttatct ccttcccggc cggggtggcg aaggcccgtt tcgtcccgtt
     2581 cgtcacatac acgtttctcg gttcgttcat ttggtgcggc gcgctagcgt acgggggaca
     2641 cgccttcggg gctcgctggg aggagctgcg ggcagtcatg cggccatttg atatcccgat
     2701 cgggattgtc ctcatggctg gtttcgccta ttacgtggtc caccacctcc ggcggggagg
     2761 aactacccac gccggttgag ctcaacgctt ggccagccag cgcgggaggc cgacgagccc
     2821 aacgagtgca accgccgaca ggctcagcag aaccagcagc cccgataacg ccaccagttt
     2881 cgctccctcc ggactgatca cccccgcacc aatttccagt gagaagccgc tcagggcgac
     2941 caacagcgca gtgaagttcc atatgccatg gaagccaacc gccatcagga agagggcgag
     3001 cagccggagt ggcctccggt tgacgatcgc ctcgatgagt ccccagctca cgagcccagt
     3061 gccgaaggca tggatcagcg gcgtcccagc tctggccacc atggaggaaa cccagtctgc
     3121 acccggttgt gccagcagca aagattcaaa taacgcgtag ccggatccgg cgagcactcc
     3181 tcctagaaag gcctggccga ccgatggccg cctctggata accggccaaa tcgacacgga
     3241 cttgaccgac tcttcgat
//
LOCUS       DATDPQ010000240         6315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1180175, whole genome shotgun sequence.
ACCESSION   DATDPQ010000240 DATDPQ010000000
VERSION     DATDPQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180175"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VI520_05385"
     CDS             <1..1088
                     /locus_tag="VI520_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37348.1"
                     /translation="HMGTLHVTTPGFRTVWDGREGGAKEGYVIMPPVLPGGSAVPGSA
                     APSRSVWRALRWIVPFTILLLALAAGVGYLVGDAERERAQRAETQRVIQEQFALGLLD
                     LEQGRHAIARQRFEYIIRLDPGFPGAAEQLAEALLGLREPPSTPAPPAVTPTPNLAPV
                     EEMLNQAQDAYDGGDWTATIETLLALRAKDPDYRAVAVDGLLYAALLNRGIQRISQDH
                     LLEEGIYDLSLAGSFAPLDEQASNWRGWAQLYLTANSYFGLNWEQAAFYFELVSTVAP
                     GIRGDVSWKYAISLRMYALELAAAGDPCLAEELMAISLDVLPDEAVAPTATAVCDACQ
                     AATAPAPPPAATPTPQETPTETPTPEG"
     gene            1099..1587
                     /locus_tag="VI520_05390"
     CDS             1099..1587
                     /locus_tag="VI520_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLF37349.1"
                     /translation="MQSWALAVAFWLHMAATVVWIGGLFFQALVLPPALRRLPERGQV
                     LEALRRRFQPLAWLSLAVLIGTGLTQMAANPNYTGILSIENSWSRAILAKHLAVGFMV
                     GVGAYQTWVVQPALARMAILEARTPQADHGPLLRRQERLARLNLTLGIAVLAFTAIAR
                     TA"
     gene            1659..2129
                     /locus_tag="VI520_05395"
     CDS             1659..2129
                     /locus_tag="VI520_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37350.1"
                     /translation="MVLQLSLWTGIVGLYLAIAGALGAIIRQLFGRGRPGWDRILAAA
                     YTILLFSAASTFVLAIAEWPDWSRLAVLGLGIFAAWVAAARPIWVPAPLWRPTFGHGY
                     LAGVMALAALWGINQAMTGSPTAPLMIAVSALAAGAASSGTSLRIPSFERSEFG"
     gene            2221..3141
                     /locus_tag="VI520_05400"
     CDS             2221..3141
                     /locus_tag="VI520_05400"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HLF37351.1"
                     /translation="MFELLFLGTSASAPSVHRGLSAQIVSFRDFRFLIDCGEGTQRQI
                     LRAGVGFRRLNRILITHGHLDHILGLAGLLSTFARWEAVDEMQIWASPWALERIHDLL
                     FGVVLRGAKPPLKIELLPIEPGNLIEDEDFRLQAFPVQHRGPGCFGFLFEQASRRPFL
                     PEKAALLGVPEGPARRELVSGRSVTLSDGKLVEPEQVLGPARRGTRLVHVGDAAATEG
                     LLEACRDADALVIEATYLETEHELAHEFGHLTARQAAELARTAGVGALYLTHISRRYR
                     EKEVLEEAQASFPNVSVVRDFDQFQVKRAD"
     gene            3186..3410
                     /locus_tag="VI520_05405"
     CDS             3186..3410
                     /locus_tag="VI520_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLF37352.1"
                     /translation="MPMYEYLCLDCNTVFDALRPMAKADAPIVCEDCESEHTSRMLSV
                     FFAHSEGRSFPTSNDAGCACGGACSCASPN"
     gene            complement(3482..3784)
                     /locus_tag="VI520_05410"
     CDS             complement(3482..3784)
                     /locus_tag="VI520_05410"
                     /inference="COORDINATES: protein motif:HMM:NF011016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLF37353.1"
                     /translation="MQTSQAHAVIIGQVQGVSFRYYTYHEALKLGLKGWVRNLRDGRV
                     EALFEGEQESVERALAWCRLGPPAARVKEVQVEWQSPSGKLEAFQVRPTANLGEPA"
     gene            complement(3807..5243)
                     /locus_tag="VI520_05415"
     CDS             complement(3807..5243)
                     /locus_tag="VI520_05415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLF37354.1"
                     /translation="MSLSDLTPILPLLLVSLWAIILLVVDLFVRRKGTTALLAAGGLL
                     LALVVLIIQSDDQQAVFNGMLVADSYAGFLQAIFLVTGLLGIAVAYDYLRRMGIERGE
                     YYPLLLFTISGMMLMSLAGDLIVVFVALELLSLPLYVLAGFARPRPESEEAAMKYFLL
                     GAFASGFLVYGIALVFGATGTTNLAGIAAELSGGAGLLLVGAVLVLVGLSFKVAAVPF
                     HMWTPDVYQGAPSAVVAFMSVGAKAGGFAALLRVFIAALPDLAAAWAPLTMGLAALTM
                     TWGNVAAIVQGNIKRMLAYSSIAHAGYILIALPAASLPAAAPGAVSAALFYLFAYLFS
                     NLGAWAVVLAVERLEGGGLQIDDYSGLSRRRPGLALAMALFMLSLTGLPPTVGFVGKF
                     FIFRAALDAGQIGLALVGVLTSLISAYYYLRVVVVMYMRTGEPETRSEGWLNATIGVT
                     ALATFVFGVLPGPILALAEHSEILGLLR"
     gene            complement(5240..>6315)
                     /locus_tag="VI520_05420"
     CDS             complement(5240..>6315)
                     /locus_tag="VI520_05420"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLF37355.1"
                     /translation="FTLVPMYFLIGIWGGERRMYAAIKFFLYTMAGSILMLLAIVWLG
                     LRANTFSVPELIAAGAIPPEIQLWLFLAFGAAFAIKVPMWPLHSWLPDAHVEAPTAGS
                     VILAGVLLKLGTYGFLRFNLSLFPQATLRLAPWMALLAVIGILYGAIVSYAQRDVKKL
                     VAYSSVSHLGFVVLGLFALNPQGIQGAILQMVNHGLSTGALFLIVGMLYERRHTRELD
                     AFGGIWKVMPVYTAITLIVTLSSMGLPGLNGFVGEFTILLGSFGSLAIGSAWFAGLAA
                     IGVILAAIYLLYMFQKLFLGPLDKPENQGLSDLNPRELLTLAPILVLIFWIGLYPRPF
                     FELMAPSVERVVALVNSAALAMR"
BASE COUNT         1191 a         2080 c         1946 g         1098 t
ORIGIN      
        1 tacatatggg gactttgcat gttaccacac ccggttttcg gaccgtttgg gatgggcgcg
       61 aagggggagc aaaggagggc tacgttataa tgccgcccgt gctcccggga gggtccgccg
      121 tccccggctc agccgctccg agccgatccg tgtggcgtgc tttgcggtgg atcgtgccct
      181 tcacgatctt gctgctggcc ctggccgccg gtgtcggcta tctggtcggc gacgccgagc
      241 gggaacgggc ccaacgagcc gaaacccagc gcgtcattca ggagcaattc gccttgggtc
      301 tgctggacct ggagcaggga cgacatgcga tcgcccgcca gcggttcgag tacatcatcc
      361 gcctggatcc cggcttcccc ggcgcggccg aacaattggc cgaggccctg ctggggctgc
      421 gcgagccgcc ctctaccccg gctcccccgg ccgtcactcc gaccccgaac ctggcgccgg
      481 tcgaagaaat gctcaatcag gcccaggacg cctatgatgg cggggattgg accgcgacca
      541 tcgagaccct gctcgctctg cgtgccaagg acccggatta ccgagcggtt gcggtagacg
      601 gattgttgta tgccgcgctt ctcaaccgcg gcattcaacg catttcgcaa gatcatctgc
      661 tcgaggaggg aatctacgat ttgagtctgg ccggaagctt tgccccgctc gacgagcagg
      721 ccagcaactg gcgcggctgg gcccagctct atctaaccgc aaatagctac tttgggctga
      781 attgggagca ggctgccttc tattttgaat tggtctccac ggtggccccc ggaatccggg
      841 gcgacgtgag ctggaagtat gcgatttcgc tccggatgta tgccctggag ctggccgcag
      901 ctggggatcc ctgcctggcc gaggagctga tggccatctc attggacgtg ctgccggatg
      961 aggccgtcgc tcccaccgcg accgcggtgt gcgacgcctg ccaggccgcg accgcccccg
     1021 ctccacctcc agcggcaacc cccaccccgc aagagacccc gaccgagaca cccacccccg
     1081 aagggtaggc gagtacccat gcaatcctgg gccctagctg tcgcattctg gctgcacatg
     1141 gcagccaccg tcgtctggat cgggggtctg ttctttcagg ccctggtcct gcctccagcg
     1201 ctgcgccggc tcccggagcg cgggcaagtt ttggaggcgc tccgccgtcg cttccaaccg
     1261 ctcgcctggc tcagcctggc ggtcctgatc ggcaccggtc tcacacagat ggctgccaat
     1321 ccgaactaca cgggaatcct ctcgattgag aattcctggt cgcgggccat cttggcgaaa
     1381 cacctggcgg tggggttcat ggtcggggtg ggtgcctacc agacctgggt ggtccagccc
     1441 gcgctggccc ggatggcgat cctcgaagcg cgtacccctc aggccgacca cggtccactg
     1501 cttcgacgac aggaacgctt ggcccggcta aacctgaccc ttgggatcgc cgtgctggcc
     1561 ttcaccgcga tcgcccgcac cgcctgatcc ggcgcggtcc ggccctccgc tgtgggcatg
     1621 caacttgcat cgcatcctgt agaacatcgg aggcaatcat ggtcttgcaa ctctcactct
     1681 ggactgggat cgtcggcctg tatctggcga tcgccggcgc gctgggtgcg atcatccggc
     1741 agcttttcgg ccggggccgg cccggctggg accgaatcct cgccgcggca tacacaattc
     1801 tgctcttcag cgcagcctcg accttcgttc tggccatcgc cgaatggcca gattggtcac
     1861 ggctggctgt gctcgggttg gggattttcg cggcttgggt ggccgccgcc cgcccgatct
     1921 gggtccctgc gccactttgg cggcccacct ttggccacgg ctatttggcc ggggtgatgg
     1981 cgctggccgc tctctggggc atcaatcagg caatgaccgg atccccaacg gctccgttga
     2041 tgatcgcagt ttccgccctg gccgccgggg cagcctcatc ggggacttcg ctgaggattc
     2101 ccagcttcga gcgatccgaa ttcggctaag ccgagcaagc gggatcagga ttgggggccc
     2161 gccgggttag tgaggtacaa tgaccggccc tattttttcc aacccagtcc tggacctcgt
     2221 gtgttcgaac ttctattcct cggtacttcc gcctccgctc catccgtcca ccgcgggctc
     2281 agcgcccaga tcgtaagctt ccgagatttc cgcttcctga tcgattgtgg tgaaggcacc
     2341 cagcgccaga tcctgcgggc cggggtcggt tttcgccggc tcaatcggat tctgatcact
     2401 cacggccacc tggatcatat tctcggactg gccggcttgc tctccacctt cgctcgatgg
     2461 gaggcagtgg acgaaatgca gatctgggcc agtccctggg cgctcgaacg gatccacgat
     2521 ctgttgtttg gggtggtgtt gaggggagcc aaaccacctc tcaagatcga attgctcccg
     2581 atcgagcccg ggaacctgat cgaagatgag gactttcgcc tgcaggcgtt cccggtccag
     2641 caccgcgggc cgggctgttt cggtttcctg ttcgagcagg cctcccgccg ccctttcctc
     2701 cctgaaaagg cggccctact cggggtgccg gagggtccgg cacggcgcga gctggtcagc
     2761 gggcgcagcg ttacgctgtc cgacggcaag ctggtggagc ccgagcaggt cctcggccct
     2821 gctcggcgcg gcacccggct ggtccatgtt ggggatgccg ccgcgactga aggattgctt
     2881 gaagcctgcc gggacgccga tgccctggtg atcgaggcca cctatctgga gaccgagcac
     2941 gagctggccc acgagttcgg ccatctgacc gcccggcagg cggccgagtt agccaggacg
     3001 gccggggtcg gggcgcttta tctcacccac atctcacgcc gttaccgcga gaaggaagtg
     3061 ctggaggagg cccaggccag cttccccaac gtttcggtgg tgcgcgactt cgaccagttc
     3121 caggtcaagc gggccgattg accgccgcat ggcacacggg tagaatcggg accaggactg
     3181 cagcgatgcc aatgtacgaa tacctctgcc tcgattgcaa cacggtcttt gacgccctgc
     3241 ggccaatggc gaaggcggac gctccgatcg tgtgcgagga ttgtgagagc gagcacacct
     3301 cgcgcatgct ctcggtcttc tttgcccatt cggagggtcg cagctttccc accagcaacg
     3361 atgccggctg cgcctgtggc ggcgcctgct cctgcgcgag cccgaactag ccagctgacc
     3421 cggaaatcaa aaagagcgcc ggcgattgcc tgccggcgct ctcgattctc agccgaaacc
     3481 ctcaggccgg ctcccccagg tttgcggtag gacgcacctg aaaggcctcc agcttcccgg
     3541 acgggctctg ccactcaacc tggacttctt tcacccgagc ggccggcggg cccagtctgc
     3601 accaagccaa cgctctttcg accgattcct gctcgccttc aaagagggct tccactctcc
     3661 cgtctcgcaa gttccggacc cagcccttca gccccaactt gagcgcctcg tggtaggtgt
     3721 aatagcggaa gctgactccc tggacctggc cgatgatcac ggcgtgtgcc tggctggttt
     3781 gcatcccctt ctcctgcgct atccggtcag cggagcagcc cgaggatctc cgagtgctca
     3841 gccagggcca gaatgggtcc gggcagaacc ccgaacacaa aggtggccag ggcggtcact
     3901 ccaatggtgg cgttcagcca gccttcggat cgagtttccg gctcgcctgt gcgcatgtac
     3961 atcacgacca cgactcgcag atagtagtaa gcggagatca gggaggtgag gaccccgacc
     4021 agcgccagtc cgatctgccc cgcatccagg gcggcccgga agatgaagaa tttcccaacg
     4081 aacccgaccg tcggaggcag tccggtaagt gagagcatga acagggccat ggctagcgcc
     4141 aggccagggc ggcggcggga cagtccggag tagtcgtcga tttgcagccc accgccctcc
     4201 aatcgctcga ccgccaatac cactgcccag gcgcctagat ttgaaaacaa ataggcgaac
     4261 aggtaaaaga gcgccgcgct caccgccccc ggggctgccg ccggaaggct ggcggccggc
     4321 agagcgatca atatgtagcc cgcgtgagca atactggagt aggccagcat tcgtttgata
     4381 ttgccctgca cgatcgcggc gacatttccc caagtcatgg ttagcgcagc cagtcccatc
     4441 gtcagcgggg cccaggcggc cgccaggtcc ggcagggccg cgatgaagac ccgcagcagc
     4501 gcggcaaaac ctccggcctt ggcccccacc gacatgaagg ccacgactgc cgagggagcg
     4561 ccctgataga cgtcgggagt ccacatgtga aagggcaccg ccgccacctt gaagctaagc
     4621 cccaccagga ccaacaccgc cccgaccagc agcaacccgg cccccccgct tagctcggcg
     4681 gctatcccgg ccaggttcgt ggtcccggtc gccccgaaga ccagcgcgat cccgtagacc
     4741 agaaagccgg aggcgaaggc tccgagcagg aagtacttca tggccgcctc ttcggactcc
     4801 ggcctggggc gggcgaatcc ggccagcacg tagagcggca gggagagcaa ctccagcgca
     4861 acaaaaacca cgatcagatc tcctgccaga ctcatcagca tcattccact gatcgtgaag
     4921 agcagcagcg gataatactc tccgcgctcg attcccatcc gccttaaata gtcataagcc
     4981 actgcgattc ccagcaaccc ggtcaccagg aagatagcct gcaggaagcc cgcataggaa
     5041 tcggcgacga gcatcccatt gaagacagcc tgttggtcgt cagattggat gatcaacacc
     5101 accagcgcga gcaggagtcc gccggctgcc aggagggcag tggtcccctt ccggcgtacg
     5161 aacaagtcta caaccagcag tatgatcgcc cacagcgaaa ccagcagcaa cgggaggata
     5221 ggggtcaggt cggaaaggct catcgcatcg cgagtgcggc cgagttaacc agggcgacga
     5281 ctctttccac ggagggcgcc atcagttcga agaacggacg cggatagagg ccgatccaga
     5341 agatcaacac caggattgga gccagagtga gcagctcgcg cggattgagg tcggacagtc
     5401 cttgattttc gggcttgtcc agcggcccaa ggaacagctt ctggaacatg tacagcagat
     5461 agatcgcggc caggattacg ccgatcgcgg ccaagccggc gaaccaggct gagccgatcg
     5521 ccaacgagcc aaacgaccca agcaagattg tgaattcccc cacaaatccg ttgagccccg
     5581 gcagccccat ggaggagagg gtcacgatca gggtgatcgc ggtgtacacc ggcatcacct
     5641 tccagatccc gccgaaggcg tccagctccc gggtgtgccg ccgctcgtaa agcattccga
     5701 cgatcaggaa caaagccccg gtgctcagtc cgtggttcac catttgcaga atcgccccct
     5761 ggattccttg ggggttcagg gcgaacagtc ctagcacgac aaaccccaga tggctgaccg
     5821 aagagtaggc caccagcttc ttcacatccc gctgggcgta ggagacgatc gcgccataga
     5881 ggatcccgat gacggccaac agggccatcc agggggccag ccgcaacgtg gcctgcggga
     5941 acagcgacag gttgaaccgc aagaagccat aggttcctag cttcaacaac actcccgcca
     6001 ggatcaccga gccggcagtc ggcgcctcca cgtgggcgtc gggcagccag gaatgcagcg
     6061 gccacatcgg taccttgatt gcaaaggccg ctccgaaggc caggaagagc cacagctgaa
     6121 tctcgggcgg gatggcaccg gccgcgatga gctccgggac cgagaaagtg ttggcccgca
     6181 gtcccagcca gacgatcgcc aacagcatca agatcgagcc ggccatggtg tacaggaaga
     6241 acttgatcgc ggcgtacatc cgccgctcgc cgccccagat tccgatcagg aaatacatcg
     6301 gcacgagggt gaatt
//
LOCUS       DATDPQ010000241         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1693960, whole genome shotgun sequence.
ACCESSION   DATDPQ010000241 DATDPQ010000000
VERSION     DATDPQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693960"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VI520_05425"
     CDS             complement(<1..258)
                     /locus_tag="VI520_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37356.1"
                     /translation="MKEMRLILAGLVTLALVLAACAPAPETTEVAPPEEAEATSVPEE
                     VAPPADLTRDLEFIMVQHALCAWDAFWCTVEQGISDAAEDLG"
     gene            complement(441..1457)
                     /locus_tag="VI520_05430"
     CDS             complement(441..1457)
                     /locus_tag="VI520_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37357.1"
                     /translation="MQAADSLGITLRAWRAVLAERSELVVLISMAAVFAFFAVSAEHF
                     LTAFALSNIVTFASINGIVVIAVALLMISGEFDLSVGSIFAVASYVFALSLIDGVAPI
                     PAMLLALIASGSLGLINGWIVTISGIPSFIATLGTMLAFRGIARAIGGGVFASYSGPR
                     LAIFDVLNGPIGLINRLSQPAANFRISVLWFVLLAAVLGIVLLRTRYGNWVFAAGGNP
                     RAANAQGVPINRVKLINFVLTGLLAGAAGVIQFGHRISVDPLRGDGLELIAVAACVIG
                     GVRLSGGFGTIVGPCVGVLLLQMLEQGLVLMRVPVQVFQAVAGSFLVLAVLMNTSLSG
                     SARE"
     gene            complement(1444..>2230)
                     /gene="iolB"
                     /locus_tag="VI520_05435"
     CDS             complement(1444..>2230)
                     /gene="iolB"
                     /locus_tag="VI520_05435"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR04378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HLF37358.1"
                     /translation="RRLRPDESWEFDSGESEYGVVLLGGVCQVETSRGEWPRVGRRPN
                     VFSGMPHALYFPRRTQVRIRALEGAVDLGYGWCATDEDHPARHISPGEVQIEIRGGGN
                     ATRQIHGLIPPGFDCHRLVAVEVYTPSGNWSSYPPHKHDEHRQDGTGNLLEADLEEIY
                     FYKLDRPEGYAVQRVYTADSRLDELILARDNDVVLVPEGFHPVVSAPGYTTYYLNFLA
                     GSAQSLANTDDPAYSWVKGTWMEKDPRLPLVTLEMEADGDAGG"
BASE COUNT          446 a          729 c          665 g          390 t
ORIGIN      
        1 tcccaggtcc tccgctgcgt cgctgatgcc ctgctcgact gtgcaccaga aggcatccca
       61 agcgcagagc gcgtgctgca ccatgatgaa ctcgagatcg cgggtaaggt cagccggtgg
      121 cgcaacctcc tctggcaccg acgttgcttc cgcctcttct ggcggcgcca cctcagtcgt
      181 ctccggcgct ggcgcgcagg ccgccaggac cagggccaaa gtcacgaggc ccgcgagaat
      241 gagccgcatt tctttcattt cagatctcct ccttcaacgg ttgacaagat tggttggtct
      301 ggctgcgatc aatttcgtgg tctctggcta tgcctttcac ctcctcggtt tgcgatgtcc
      361 cactcctcca agttcaggca aggggctttg gcgataggta ctctaccagg gattcttgcc
      421 ctcggtgtcc ctctggctga tcactccctc gccgacccac tcagcgaggt gttcattagg
      481 acggccagga ccaggaagga tcccgcgacg gcttggaaaa cctgaaccgg caccctcatc
      541 aataccagcc cctgctccag catctgcaac agcaggaccc cgacgcaagg gcccacgatc
      601 gttccgaacc caccactgag ccgcacgcct ccgattacgc aagccgccac cgcgatcagc
      661 tccagtccat cccctcgcag cggatccacg ctgattcgat gcccaaattg aataacccca
      721 gccgcgccgg ccagcagccc ggtaagcacg aaattgatca gcttgacccg gttgatgggc
      781 acaccctggg cattggccgc ccgagggttc cctcccgccg caaacaccca gtttccgtag
      841 cgggtgcgca gcagaacgat ccccaagacg gccgcgagca gcacgaacca caagaccgag
      901 atccgaaagt ttgccgccgg ctgggaaagc cggttgatga gcccgatggg gccgttgaga
      961 acgtcgaaga tggccagccg agggccgcta taggaagcga acactccgcc cccgatggcc
     1021 cgtgcaatac cacggaacgc gagcatcgtg ccaagcgttg caatgaagga agggatcccg
     1081 gatatggtga cgatccaccc gttgatcaga ccgagagacc cgctagcgat cagagccagt
     1141 agcatcgccg ggatcggggc gaccccgtcg atcagcgata gggcgaatac atagcttgcc
     1201 acggcaaaga tcgagccgac ggataagtcg aattcgcccg agatcatgag cagcgcaaca
     1261 gcgatcacga cgatgccgtt gatgctggcg aaggtaacga tgttcgagag ggcgaaggcg
     1321 gtgagaaagt gctctgccga tacggcaaaa aaagcgaaaa cggccgccat cgagatgagc
     1381 accacgagtt cggaccgctc ggccagcacc gcccgccagg cgcgcaatgt gatccccagc
     1441 gaatcagccg cctgcatccc catcagcttc catctcgagc gtgacgagcg gtagtcgcgg
     1501 gtctttctcc atccaggtcc ctttgaccca gctgtaagca gggtcgtcgg tgttcgccag
     1561 cgactgggcg ctgccggcca ggaagttcag gtagtaggtg gtgtacccgg gagcagaaac
     1621 gaccgggtgg aagccctccg ggacgagtac gacatcgttg tccctggcca ggatgagttc
     1681 gtccagccgg ctgtccgcgg tgtataccct ttggacggcg tacccttctg gcctgtccag
     1741 cttgtagaaa tagatctctt ccaggtcggc ttccagcagg ttgccggtcc catcttgccg
     1801 gtgctcatcg tgtttgtggg gagggtaaga gctccagttt ccggagggcg tgtagacttc
     1861 cactgccacc agccggtgac aatcgaagcc gggcgggatc aggccatgga tctggcgggt
     1921 ggcattccca cccccgcgga tttcgatctg aacttcaccc ggcgagatat ggcgggctgg
     1981 gtgatcctcg tcggtggcgc accagccata tcccaggtca accgcaccct ccagggcgcg
     2041 gatccgcacc tgggtccggc ggggaaagta gagggcatgg ggcatccctg aaaagacgtt
     2101 cggccggcgg cccacccgcg gccattcgcc gcgcgaagtc tccacctggc acacgccccc
     2161 gagcaggacg accccgtatt cgctttcgcc gctgtcaaac tcccagctct cgtccggccg
     2221 caggcggcgg
//
LOCUS       DATDPQ010000242         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_115258, whole genome shotgun sequence.
ACCESSION   DATDPQ010000242 DATDPQ010000000
VERSION     DATDPQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115258"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VI520_05440"
     CDS             <1..533
                     /locus_tag="VI520_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HLF37359.1"
                     /translation="LPIAVKLSPFFSSLPHMARRLAGAGAQGLVLFNRFYQPDIDIES
                     LRTEPTLELSSSSELRLPLRWIALLFGNVAVDLALTTGVHTVEDAIKGLMAGASVTMM
                     ASELLQNGIQRIGEILSELEVWLEVHEYETLVPLIGSMSVLRSGQSAAFERANYISVL
                     RSYLPDAALPTIDQTS"
     gene            complement(594..821)
                     /locus_tag="VI520_05445"
     CDS             complement(594..821)
                     /locus_tag="VI520_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37360.1"
                     /translation="MASSGWVVVKQGWCDQGGQEARLLERRVYPAEILPDTVGFRVTA
                     RRCDRDVACNLAEFPCCWAFTRPELDPFGLA"
     gene            1108..>2070
                     /locus_tag="VI520_05450"
     CDS             1108..>2070
                     /locus_tag="VI520_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HLF37361.1"
                     /translation="MALFAAIGGLAGRSNPASRLPANLSAPVQQGGDYAGPDTCGACH
                     EEIHTVWTDSRHANAFSSPIFQQNWAEIGSEFTCLTCHTTGYDPATNQYASEGVTCEA
                     CHGPFQEGHPVEPMPIQADAELCATCHTTTKNEWDASKHGQVGIQCQSCHNPHSQQPR
                     AATITELCTNCHKERGETFTHGTHANAGLECSSCHMYTDPQSGPPIQGLVATGHTFAV
                     GSQACIGCHQDTVHTRDTILQLSGQVAALEEVDLQALQQQLQQQEQAIADLEAGRSAR
                     LYVGLAQGAIIGLATGGVAAWIISRRLKIVEPEAEPEQQGPPGDA"
BASE COUNT          393 a          702 c          613 g          362 t
ORIGIN      
        1 cgcttccgat cgcggtgaag ctcagcccgt ttttcagctc cttgccgcat atggcccgcc
       61 ggctggccgg cgcgggggcc cagggactgg tgctcttcaa ccggttctat cagcccgaca
      121 tcgacatcga aagcctgcgc accgagccga ccttagagtt gagcagctcc agtgagttac
      181 gccttccctt gcgttggatt gccttgcttt tcggaaatgt ggccgtcgac ctcgccctga
      241 ccaccggagt gcatactgtg gaagatgcca tcaaaggtct gatggccggg gcctcggtga
      301 cgatgatggc ctccgagttg ctccagaatg gaattcagcg gattggggag attctgagcg
      361 aactcgaggt gtggctcgag gtgcacgaat acgaaacgct cgtcccgctc atcgggagca
      421 tgagcgtgct ccggtcggga cagtcggctg ccttcgagcg ggccaactac atatcggtcc
      481 tgcgctctta cctgccggac gccgcgctgc cgacgattga tcaaacttcc tgatcgaaga
      541 acacgcctcc cgggggaggc gtgtccactt ccatggggtg cgggggcctt gtctcaggct
      601 aatccgaaag gatcgagctc cgggcgggtg aaggcccagc aacaggggaa ttctgccaga
      661 ttgcaggcta cgtcacggtc gcagcggcgg gccgtcaccc ggaaccccac ggtatccggc
      721 aggatctccg ctggatagac ccgccgctcc agcaggcggg cctcctgacc accctggtca
      781 caccagccct gcttgaccac gacccatcct gaggatgcca tatgccctcc actaaaggac
      841 cgtcgcaggc tgttccccct ttcgccatcc taaagcgggg ccgtccgtgc cgaaagtgac
      901 caatgccatc caactggccg ccgatttacg ctgcatatcg ggaccctgtt cgtgatgaaa
      961 atcacaaatt agccgtgatc gacggtactc atggggtccc attcagccga ttactatcct
     1021 atcagccgcc gaaggccacg aactggcatg cgaatccctc gttctaaact gcttcgcgcc
     1081 ccaatcttgc tggctgttgg agtcccagtg gcactcttcg ccgcgatcgg cggcctggct
     1141 ggccggtcca acccggcgtc gcggctcccg gccaatctct ccgctccggt gcaacaaggc
     1201 ggcgactacg ccggcccgga cacctgcggg gcatgccatg aagagatcca cactgtctgg
     1261 accgattccc ggcacgccaa tgccttctcc tcgccgatct tccagcagaa ttgggccgag
     1321 atcggcagcg agtttacctg cctgacctgc cacaccaccg gctacgaccc cgcgaccaac
     1381 caatacgcct ccgagggggt gacctgcgag gcctgccacg gacccttcca ggaggggcac
     1441 cccgtcgagc cgatgcccat ccaggccgac gctgagctgt gtgccacctg tcatacgacg
     1501 accaagaatg agtgggacgc cagcaagcat ggccaggtcg gcatccagtg ccagtcctgt
     1561 cacaacccac actcccagca accccgggcc gccaccatta ccgaactgtg caccaactgc
     1621 cacaaggagc ggggcgagac cttcactcac ggaacccacg ctaacgccgg gctggagtgc
     1681 agcagctgtc acatgtacac cgaccctcag agcggcccgc ctatccaggg cctggtcgcc
     1741 accgggcata ctttcgcggt cggatcccaa gcctgcattg gctgccacca ggacaccgtc
     1801 catactcgcg atacgatcct acagttgagc ggccaggtgg ccgcactcga ggaagtcgac
     1861 ctccaagccc tgcagcagca gctccagcag caggagcaag ccattgcgga cctggaggcc
     1921 ggccggtctg cccggctcta tgtcgggtta gcccaggggg cgattatcgg gttggctacc
     1981 ggcggggtcg cggcctggat catcagccgg cgactgaaga tcgtggagcc ggaggccgaa
     2041 ccggagcagc agggtccgcc aggcgatgcc
//
LOCUS       DATDPQ010000243         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3280351, whole genome shotgun sequence.
ACCESSION   DATDPQ010000243 DATDPQ010000000
VERSION     DATDPQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3280351"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..>2971)
                     /locus_tag="VI520_05455"
     CDS             complement(7..>2971)
                     /locus_tag="VI520_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF37362.1"
                     /translation="LELHGYKSFASKTVFEFGDTITAVVGPNGSGKSNIADAIRWVLG
                     EQAYSTLRGRKTEDMIFAGSEARPRAGMATATITFDNRDNWLPIDFAEVAVSRRAHRD
                     GENEYLLNGQRVRLRDIQDLLGSVGLAERNYTIIGQGLVDSVLSLRPEERIKLFEEAA
                     GITGHRIRKEEAIRRLEKTQSNLERAKDIVTEIRPRLRSLAQQAERASVYDRLREELS
                     AALHSWYGFHWHRLQAELQAAEQRAAQSAALRDEVLTHGESSHQTLIATDARAAALRA
                     EIEPAEATYRARASDREDLERSQAVAEERVRWLEEEEQSLNREMLAQERVRGELAERV
                     AAARSEAEGKAAGLTDRTVHARRPQDEVEQQLKEVLGRIESIDRESRQLAQQQAASET
                     RLKLEDSPTQNSNLIAGRMREAVERGLLSGWMGELNGQLEMEPAHESAISAALDGFSR
                     GIVFRSGHHLDEALDWLNREDSKERLALLPQSRERELPELEVPADPDCLGRAVDLVRV
                     PPEFQGAVELLLGRTLVVRDRRSAKRVVQGLPLDARVVTLSGQVFYPAGQVLVPVNGK
                     SSNSSDQTRAALRELMLRAQQLEAERRAASAQRETLEGEWKSRLSQELLQATQAKVEG
                     LEEQYRETEVTAARQRERLSRNQTAQSTTRLEIYDGRSRLGRVRTELLASSAAVNELS
                     AALAEIEASRVGLQADEAQSREARREAEQDYTQAQIDIARMQQSASDMERRILEDFSL
                     VNPEPEAWGSDPTPLSMAGLVDQLLPVDQLPPELETELNRKRGQLRRLGSVDPAAQLE
                     FEEVKGRHDFLQEQIQDLVQAEGQLTEVIQELDELMAQEFRRTFEAVAEAFEGFFVRL
                     FAGGSARLEYLGEEGIEIQVKLPGHRNQGLAMLSGGERSLTAVALVFALLRLSPTPFC
                     VLDEVDAMLDERNVERFGLILRELSERTQFLVITHNRQTIQAAEVLYGISLGRDNSSD
                     VISLRLDQVEERIAA"
BASE COUNT          490 a         1006 c          879 g          596 t
ORIGIN      
        1 cgcgagttat gctgcgatcc gctcctcaac ttgatccagc cgcaggctga tgacatcgct
       61 cgagttgtct ctacccaggc taatcccgta gaggacttcg gcggcctgga tcgtttgccg
      121 attatgggtg ataaccagga actgggttcg ctcactcagc tcgcgcagga tcaatccaaa
      181 tcgctcgaca ttgcgttcgt ccagcatcgc gtctacctcg tccaacacgc agaatggagt
      241 cggcgaaagt ctgagcaggg caaagaccag tgccaccgcg gtcaagctgc gttcgccacc
      301 cgagagcatg gccaatccct ggttacggtg cccaggaagc ttgacctgga tctcgattcc
      361 ttcttctccg aggtactcta gtcgggctga tccgccggca aacagccgaa cgaaaaagcc
      421 ttcaaaggcc tccgccactg cctcgaatgt ccggcggaat tcctgggcca tcagctcatc
      481 cagctcctgg atcacctcgg ttagctggcc ctcggcctga acaagatcct gaatctgttc
      541 ttgcaggaag tcgtggcggc ctttgacctc ttcgaactcc agctgggcgg ccggatcgac
      601 cgacccgagc cgacgcaatt gccctcgctt ccgattgagc tcggtttcca actcgggagg
      661 cagttgatcg accggtagca actggtcgac gagccccgcc atgctcaacg gggttggatc
      721 agagccccag gcctccggct ccggattgac gagactgaaa tcttccaaga tcctgcgctc
      781 catgtccgat gcagattgct gcatacgggc aatatcaatc tgcgcctgag tgtagtcttg
      841 ctccgcttct cgccgagcct cccggctctg agcctcgtcg gcctgcagcc cgacccggct
      901 cgcctcgatc tccgccaggg ccgcgctgag ctcgtttacg gcggccgagg aggccaagag
      961 ttcggttctt acccttccga gtctgctccg gccatcgtag atctccaggc gggtcgtgct
     1021 ctgggcggtc tgattccttg acagccgttc ccgctgcctg gccgcggtaa cctccgtttc
     1081 acgatattgc tcctccaagc cttcaacttt ggcctgggtt gcctgcaaga gctcctgcga
     1141 gagtcgcgac ttccactcac cctccaaagt ctcccgctgc gccgacgcgg cgcggcgctc
     1201 ggcttcgagc tgctgcgctc gcagcatgag ctcccgaagg gcagctcggg tctgatcgga
     1261 cgaattggaa gacttgccgt tgaccgggac cagtacctgg ccagcgggat agaacacttg
     1321 cccggacaag gtcacgaccc gggcatccaa ggggagcccc tggacaaccc gcttggccga
     1381 gcgccggtcc cgcaccacca gggtccgacc cagcagcagc tcgaccgctc cttgaaactc
     1441 cggcggaacc cgcaccaggt ctacggcccg ccccaagcag tcagggtcgg cagggacctc
     1501 cagctccggc agctcgcgtt cgcgggactg tggaagcaga gccagcctct ctttggagtc
     1561 ctcgcggttt agccaatcca aggcttcatc aaggtggtgt ccgctgcgaa agacaatccc
     1621 ccgactgaat ccatccaggg cggccgagat cgcggactca tgggccggtt ccatctccag
     1681 ctgaccattg agttcgccca tccagccgga aagcaaccca cgctcgacgg cctcccgcat
     1741 ccgaccggcg atgaggttcg aattctgggt cgggctgtct tccagcttaa gcctggtctc
     1801 agatgcggcc tgctgttggg ccaactggcg ggactctcga tcgatcgatt caatccgccc
     1861 gaggacctcc ttcaactgct gctcgacttc gtcctgcggc cggcgggcat ggaccgttcg
     1921 atcggttaag ccggctgcct tgccctcggc ctcggagcgg gccgcggcca cccgctcggc
     1981 cagttcgcct cggacgcgct cctgtgccag catttcgcgg ttgaggctct gttcttcctc
     2041 ttcgagccag cgcactcgct cctccgccac cgcctggctg cgttcgagat cctcccgatc
     2101 ggaggcccgg gcgcggtagg tcgcttcggc cggctcgatc tccgcccgga gcgctgccgc
     2161 tcgggcgtcg gtcgcaatca aggtttggtg ggagctctca ccgtgggtga gcacttcgtc
     2221 ccggagggcc gccgattggg cggctcgctg ctcggcggcc tgcagctcgg cctgcaatcg
     2281 gtgccagtgg aaaccgtacc aactgtgcaa ggccgcgctc agctcttccc gaagccgatc
     2341 gtacacggag gcccgttcgg cctgctgggc gaggctccgc agccggggtc ggatttcggt
     2401 cacgatatcc ttggcccgct cgaggttgct ctgggtcttc tccaagcgcc tgatcgcctc
     2461 ttccttgcgg atgcgatggc ctgtgatccc ggcagcctct tcgaacagct tgatccgctc
     2521 ctccggccga agtgaaagta ccgagtcaac cagtccctgg ccgatgatgg tgtaattgcg
     2581 ctcggccaat ccgacgctac ccagcagatc ttgaatgtcg cgcaaccgta cccgctggcc
     2641 gttcagcagg tattcgttct cgccgtcccg atgagcccgg cggctgacgg ccacctcggc
     2701 gaagtcgatg ggcagccagt tgtcccggtt gtcaaaggtg atcgtggccg tcgccatgcc
     2761 tgcccggggt cgggcttccg acccggcgaa aatcatgtcc tcggtcttgc gaccgcgcag
     2821 ggtgctgtag gcctgctcac cgagaaccca gcgaatggca tcggcgatgt tggatttgcc
     2881 gctgccgttg gggccgacga ccgcggtgat cgtatccccg aactcgaaca cggtcttgct
     2941 ggcgaaactc ttatagccgt gcagttcaag c
//
LOCUS       DATDPQ010000244         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_900061, whole genome shotgun sequence.
ACCESSION   DATDPQ010000244 DATDPQ010000000
VERSION     DATDPQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900061"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VI520_05460"
     CDS             <1..825
                     /locus_tag="VI520_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLF37363.1"
                     /translation="TAVARDAERIRRAGDGLRRLGIGGTATGTGLNAHPDFPRLMVGR
                     LAELTGFELRSSTDFFESMQSMADMADFSASIRTLAITLTRIANDFRLLSSGPATGLD
                     EIRLPAVQPGSSIMPGKVNPVLPEMLNMAMYHVIGCDTTVLLAAQAGQLELNVMMPVL
                     AHNLFEMMQVTIGAVQAFTDRCVRGVLANREKAQSWLERNAIIVTALNPLIGYAAGAE
                     LVKESLARSQTVFEVARQRLEKGELRHRDGSRSVTEAELEAALGNLRRLTEGGIVG"
     gene            complement(847..1716)
                     /locus_tag="VI520_05465"
     CDS             complement(847..1716)
                     /locus_tag="VI520_05465"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37364.1"
                     /translation="MNQSFEREVRPVPPERAAPAPVPLPRPDRDRAGALVALILIAYS
                     LVRIIALPLQAIEFELFGLLIRVDFDARFVMLTLAAAVAASGVDWVLTSHPLRRPVGL
                     EPRILPGLAALGVGAILAGLPGWPAWPIGLGLGGLLLYAVLYAEFVVWDPADPRTATA
                     SLGLRWLGILLAVGVAFAVRVGGLRAVFSVPVLFAATGLICWRVLRLDRTRKSIWPYA
                     VTAGWITAQLAWGLHYWPLRPIPAALLLGLAAYVSIGLGRAHLLGRTEPRAIYEFGGV
                     ALAVLAAVLLLGG"
     gene            complement(1722..2459)
                     /locus_tag="VI520_05470"
     CDS             complement(1722..2459)
                     /locus_tag="VI520_05470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF37365.1"
                     /translation="MRSSVPAPPEPPTHGLVSIILPTYNEAENILATVGGVLQAVGDP
                     VEVIVVDDDSPDETWKLVEELGDSRVRVVRRRGVRGLASAVNRGIIEASGELVGWMDV
                     DLSMPAGLLSEMVRHAREVPVVIGSRYVAGGGDDRPRFRVMTSRWVNGLASLVLGGGV
                     KDYDSGFIVVRREVFDRVTLLPVGYGAYFIDFIYHCRKRGLAVLELPYTLVDRTAGSS
                     KSMPNLWRFLLQGLGYVLTIFQARLRP"
     gene            complement(2431..>3018)
                     /locus_tag="VI520_05475"
     CDS             complement(2431..>3018)
                     /locus_tag="VI520_05475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF37366.1"
                     /translation="YQVTGVDLSPAMLREAERKLAGLSSDSVPEFVLGDIRELELHQE
                     FDAALMMFAVIGYQASEADLQLALQSVRRHLRPGGLFVGDFWYGPTVLRIAPSDRVKQ
                     VDLGGEQLVRTATTELDLGKHLAAVHYRLARTDQDGETRIDEETHTMRFFFPMEIEQE
                     FRSANLELAGMYPFPELDAPLDSTVWNALFCARAA"
BASE COUNT          593 a          990 c          965 g          470 t
ORIGIN      
        1 accgcagtgg cgcgcgacgc agaacggatc cggcgggcgg gggatgggct gcgccgcctg
       61 gggatcggcg gcaccgcaac cggaaccggg ctgaacgccc atccggattt cccgcggctg
      121 atggtcgggc ggcttgctga actcaccggc ttcgagctcc gcagctcgac cgacttcttc
      181 gagagcatgc agtccatggc ggacatggcc gacttctcgg cctccatccg gacgctggcg
      241 atcaccctga ctcgaattgc caatgacttt cggctgttat cgtccggacc ggccaccggc
      301 ctggacgaaa tccgccttcc ggcagttcaa ccgggctcca gcattatgcc gggcaaggtc
      361 aatccagtgt tgcccgagat gctcaacatg gcgatgtacc acgtgatcgg ttgtgacacg
      421 actgtgctgt tagctgccca ggccgggcag ctggagctca acgttatgat gccggtgctg
      481 gcgcataact tgttcgagat gatgcaggtc acgatcggtg cagtccaggc attcaccgat
      541 cgctgcgttc ggggggtgct cgccaatcgg gagaaggccc agagctggct cgaacgtaat
      601 gcgatcatcg tcaccgccct aaacccgctg atcggctacg ctgccggggc cgaactcgtc
      661 aaagagtcgt tggcccgaag ccagacggtc tttgaggtag cgcgccaaag gttggagaag
      721 ggcgagctcc gccatcggga cggaagccgg tcggttaccg aagccgagct cgaagccgca
      781 ctcggcaatc tgaggcggct gaccgaaggt gggattgttg gctgaggctt cggaggcccg
      841 ctcggctcag cccccgagca agagcaccgc ggccagcacc gccagcgcca cgcccccaaa
      901 ctcgtagatc gcgcggggtt cggttcgccc gagcagatga gcccttccca ggccgatcga
      961 gacgtatgcg gccaggccca gcagcagcgc ggccggaatg gggcggagcg gccagtagtg
     1021 cagcccccat gccaactggg ccgtgatcca tccggcagtc accgcgtagg gccagatcga
     1081 tttccgggtc cgatcgagcc ggagcactct ccagcaaatg aggccggtgg ccgcgaagag
     1141 gaccggaaca ctgaagactg cccgcaagcc gccgacccgg accgcgaagg cgactcctac
     1201 cgccaacagt atccctagcc agcgtagtcc gaggctggcg gtcgcggtcc gggggtctgc
     1261 cggatcccac accacaaact ccgcgtacag cacagcatag agcagcaggc cgcccagccc
     1321 caggccaatc ggccaagccg gccatccggg cagtccagcc agaatcgcac caactcccag
     1381 cgccgccaac ccggggagga ttcgcggctc gagtccgacc gggcggcgga gcgggtggga
     1441 ggtcagcacc cagtccaccc cgcttgcggc gaccgcggcg gccaaggtca gcatcacaaa
     1501 gcgggcgtcg aaatcgactc ggatcagcag gccgaagagc tcaaattcga tggcctgcag
     1561 cggcagcgca atgatccgga ccaagctgta ggcgatcagg atcagtgcca ccaacgcgcc
     1621 ggcccgatct cgatcggggc gggggagagg gaccggcgcc ggggcggctc gctccggcgg
     1681 cacgggccgc acctcccgct cgaagctttg attcatgcgg actagggtct cagcctggcc
     1741 tggaaaattg tcaagacgta gccaagcccc tggagcaaga atcgccagag gttgggcatc
     1801 gacttagaac tgcccgcggt ccggtcgacc agggtgtagg ggagctccaa cacagcaagc
     1861 ccacgctttc gacaatggta aatgaagtcg atgaagtatg cgccgtatcc gaccggcagc
     1921 agagtcactc gatcgaacac ttcgcggcgg actacgataa acccgctgtc atagtctttc
     1981 actccgccgc ccagcacaag cgaggccaag ccgttcaccc agcgactggt catgacccgg
     2041 aagcggggcc ggtcgtcgcc gcctccagcg acgtatcgag atccgatgac caccggaacc
     2101 tcgcgggcat gccggaccat ctctgagagc agtccggccg gcatgctgag gtcgacgtcc
     2161 atccagccga ccagctcgcc gctcgcttcg ataatccccc ggttgacggc cgaggccagc
     2221 ccgcgtaccc ctcggcgccg cacgactcgg acccgggaat ctccaagttc ctcgaccagt
     2281 ttccaggtct catcgggaga atcgtcgtcc acgacgatga cttcgaccgg gtcgccgacc
     2341 gcctggagca ccccgccaac ggtcgccaga atgttctcgg cctcgttgta cgtcgggagg
     2401 atgatcgaaa ccaggccgtg ggtcgggggt tcaggcggcg cgggcacaga agagcgcatt
     2461 ccagacggtt gaatccagtg gggcgtccag ttcgggaaag gggtacatac cggccagttc
     2521 caagttcgcc gaccggaact cctgctcaat ttccattggg aagaagaacc gcatggtgtg
     2581 ggtctcttcg tcgattcggg tctccccatc ttgatctgtt cgggcgagcc gatagtggac
     2641 cgcagccaga tgcttgccga gatcgagctc ggtggtcgcg gtacggacga gctgctcgcc
     2701 gccgagatcg acttgcttga ctcgatcgga gggggcgatc ctcagcacag ttgggccata
     2761 ccagaagtct ccgacgaaaa gacccccggg tcgaagatgg cgccgcaccg actgcagggc
     2821 gagctgcaga tcggcctcgc tggcctggta tccgatcacc gcgaacatca tcagggccgc
     2881 gtcaaactcc tggtgaagct cgagctcccg gatgtcgccc aaaacgaact cgggcaccga
     2941 atccgatgaa agccccgcca gctttcgctc ggcctcccgc aacatcgccg gcgagagatc
     3001 gaccccggtg acttggta
//
LOCUS       DATDPQ010000245         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1436975, whole genome shotgun sequence.
ACCESSION   DATDPQ010000245 DATDPQ010000000
VERSION     DATDPQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436975"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VI520_05480"
     CDS             complement(<1..573)
                     /locus_tag="VI520_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF37367.1"
                     /translation="MSSGPPPAIRLDRVSRLFGEVKAVDDVSLDILDGEFFTLLGPSG
                     SGKTTSLRLIAGFDHPSAGRILLHGEDVTRLPPYEREVNTVFQDYALFPHMSVGENIG
                     YGLMIRHAPKPERARQVAEMMELVRMPGLEDRRPSQLSGGQRQRVALARALILRPRVL
                     LLDEPLGALDLKLRQQMQVELKSIQEQVGIT"
     gene            complement(570..2015)
                     /locus_tag="VI520_05485"
     CDS             complement(570..2015)
                     /locus_tag="VI520_05485"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HLF37368.1"
                     /translation="MNYQLWINGKWQPTKGGGTMVILNPATEEAIAEVVDGSREDVDR
                     AVQAAKTAFYDGRWSKRTPSDRSKILWKMADLLEARMADFARAESENTGKPYSFVSLG
                     ADLPFTVDNLRFFASAARDMHGSHAGEYLAGYTSIFRREPVGVVGQITPWNYPLMMAI
                     WKIGPALAAGCTAVLKPAPTTPLTTLMLAELSKEAGLPDGVFNVVTGGNESGQALVEH
                     PDVRMVSLTGSPRTGMRVMETASKTLKRVHLELGGKAPLLVFEDADADLVVSKVTLAA
                     TFNTGQDCTAATRVYVDKGRYKTLQQSLVESMRAVKLGEPFADGVAMGPLISAAQRER
                     VQGFVERAKAGGARVLTGGGIPKGISKGFYYEPTVVIDAAQQSEIIQSEVFGPVLTIS
                     PFNDAEEALDLANDVAYGLSASIFTKDIGRAMKLSANIEAGTVWVNDHIPLTSEAPHG
                     GFKHSGFGKDLSAEAMGDYQITKHVMVSHTG"
     gene            complement(2115..>2840)
                     /locus_tag="VI520_05490"
     CDS             complement(2115..>2840)
                     /locus_tag="VI520_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37369.1"
                     /translation="MYNTEVFTEPPTSWNVVFEETTLPDGQSNAGRIEAYDGPIYIAD
                     AALYLMYHNPELGIQDPYELTRDQFNAALDLLRVQRGLVNRYWHDVVLQVDDFTNEGV
                     VASSSWPYQVNLLAVDQPVSSVIPVEGATGWADTTMMHTNAPHPNCAYKWLEHSLNTQ
                     LQGDLAYWFGSVPSVPAACDTNEMLGPTGCDVNGLGNFEQISFWKTPSAACGFDPAGD
                     AGPNDCVPYHEWVTNMIAVIGGR"
BASE COUNT          503 a          930 c          863 g          544 t
ORIGIN      
        1 ggtgatcccc acctgttcct ggatggattt cagctcgacc tgcatctgct ggcgcagctt
       61 gaggtccagt gcccccaggg gttcgtccag cagcagcacc cgcggtcgca gaatgagggc
      121 ccgagccagc gccacccgct ggcgttgtcc gccagataat tgggacggcc ggcgatcctc
      181 caggccaggc attcggacca gctccatcat ttctgcaacc tgcctggcac gctccggctt
      241 gggggcgtgt cggatcatca gaccatagcc gatattttcg ccgaccgaca tgtgagggaa
      301 gagggcgtag tcctgaaaga cggtgttcac ctcccgctcg tagggcggaa ggcgagtcac
      361 gtcttccccg tgcagcaaga tccggccggc gctcggatgg tcgaacccgg caatcaaccg
      421 cagggaggtc gttttgcctg aaccggaggg gcctaacagc gtgaaaaact cgccatccag
      481 gatgtcgagg ctgacatcgt cgacggcttt gacctctccg aataggcggc tgacccggtc
      541 cagccggatc gccggcggag ggccggagct catccggtgt ggctgaccat gacgtgcttg
      601 gtgatctggt agtcccccat cgcctcggcc gataggtcct tgccaaaacc ggagtgtttg
      661 aaccccccat ggggggcttc agaggtgagt ggaatgtgat cgttgaccca gaccgtgccc
      721 gcctcaatat tggccgagag cttcatggcc cggccgatgt ccttggtgaa aatcgaggcc
      781 gacaacccgt aggccacgtc gttcgccaga tccagcgcct cctcggcgtc gttgaacggg
      841 ctgatggtca gcaccggccc gaacacttcg ctctggatga tctccgactg ttgcgcggcg
      901 tcgatcacga cggtcggctc gtagtagaag cccttcgaaa tccccttggg gattccccct
      961 ccggtcaaca cccgggcgcc gccagccttg gcccgctcga caaagccctg cacccgctcg
     1021 cgctgggccg ccgagatcag cgggcccatc gccacgccat cggcgaacgg ctcacccagc
     1081 ttcaccgccc gcatgctttc caccagggac tgctgcagag tcttgtaccg acccttgtcc
     1141 acgtacaccc gggtggctgc cgtgcagtcc tgcccggtgt tgaaagtcgc cgccagggtc
     1201 accttggaga cgaccaggtc ggcatccgcg tcctcaaaga ccagcaaagg ggccttgccc
     1261 cccagttcca gatggactcg tttcagggtt ttcgaggcgg tctccatcac ccgcatcccg
     1321 gtccgcggcg agccggtgag gctcaccatc cggacatccg ggtgctcaac caatgcctgg
     1381 ccgctttcgt tgcctcccgt cactacattg aagaccccat ccggcaggcc cgcctctttg
     1441 gagagctcgg cgagcatcaa agtggtgagg ggagtggtcg gggctggttt caacaccgcg
     1501 gtacagcccg cagccagcgc cgggccgatt ttccagatcg ccatcatcag cggatagttc
     1561 cagggggtga tctggccgac caccccgacc ggttcgcggc gaaaaatcga ggtgtaaccg
     1621 gcgagatact ccccggcatg cgaaccatgc atatcccggg cggccgaggc gaagaagcgc
     1681 aggttgtcga ccgtgaaggg caggtcggcg cccaggctga cgaagctgta gggctttccg
     1741 gtgttctccg actcggctcg ggcgaaatcg gccatccggg cttccaacag gtctgccatc
     1801 ttccacagga tcttggaccg gtcgctcgga gtccgtttcg accaacgacc gtcgtagaag
     1861 gcggtctttg cggcctgaac ggcccgatca acgtcctccc gactgccatc gacaacctcg
     1921 gcaatggctt cttcggtggc cgggttgagg atcaccatcg tgccgccacc cttggtcggc
     1981 tgccacttcc cgttgatcca cagttggtag ttcattcgtt cctccagctt cagagacctc
     2041 gccctcactc cctccccacc cgtgggtggg gagaggctgg tggcgaaatc cgccgtccag
     2101 tcagtctcgg gagatcagcg acccccgatc accgcgatca tgttcgtgac ccactcgtga
     2161 taggggacgc agtcgttcgg acctgcgtct ccagccgggt caaaaccaca ggccgcggac
     2221 ggagtcttcc agaaactgat ctgctcgaaa ttccccagcc cgttgacatc gcagcccgtc
     2281 gggccgagca tctcattcgt atcacaggcg gccgggacag agggtaccga gccgaaccag
     2341 taggccaaat cgccctggag ctgcgtgttc agtgagtgct ccaaccactt gtacgcgcag
     2401 ttcggatgcg gcgcgttggt gtgcatcatc gtcgtgtccg cccagccggt cgccccttca
     2461 accgggatca cgctgctgac cggttggtct acggcgagca ggttaacctg atatggccag
     2521 gagctggagg ccactacgcc ctcattggtg aagtcgtcaa cctggaggac gacgtcgtgc
     2581 cagtatcgat tcaccagccc acgctgcacc cgcagcagat ccagcgctgc attgaactgg
     2641 tcccgggtca gctcgtaggg gtcctggatg cctagctccg ggttgtggta catcaagtac
     2701 agcgccgcat cggcgatgta gatcggacca tcgtaggcct cgatgcgccc ggcgtttgat
     2761 tgtccgtcgg ggagggtcgt ttcctcgaag accacgttcc aactcgtcgg cggctcggtg
     2821 aagacttccg tgttgtacat
//
LOCUS       DATDPQ010000246         5842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_900505, whole genome shotgun sequence.
ACCESSION   DATDPQ010000246 DATDPQ010000000
VERSION     DATDPQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900505"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1617
                     /locus_tag="VI520_05495"
     CDS             184..1617
                     /locus_tag="VI520_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF37370.1"
                     /translation="MVTRLRPTQLPLRLRLTLWYLLSLGLILLLFGLLLYWQVQHNLL
                     VQVDSALQLAAVQSSATVGIQDGGLDFQNADASPALARLSEDTAIRLLADDGTVMDSL
                     GRAAEAPVLTPASGYQTYFDGDDTWRVYGERVDLPDAAAGGWLQVTQSLESVQQTLLL
                     LRLQLLWGVPLALLLAGAGGYFLDSRAMRPIDRITRTAQAINSSDLSLRIGHSGPADE
                     LGRLAATFDEMLDRLQSAFDRERRFTADAAHELRTPLTALKGRIGVTLSQPRQATEFC
                     DSLQEMERQVDRLIRLSSDLLFIARLDQGQVKRRQDPIALDDLLAAVVEQLRPLAEAK
                     AISLPEPDASGLTVPGDMDLMIRLFLNLLDNAIKFTPPGGRVSVEAQRSGEQVTIAIR
                     DTGPGIPAVHRAHLFERFFRMGGDRSRADGQGGAGLGLAIAHEIARAHGGSLTVKSAL
                     GDGTTFFVKLPRSPLPSEVPALRHSVS"
     gene            1711..3003
                     /locus_tag="VI520_05500"
     CDS             1711..3003
                     /locus_tag="VI520_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37371.1"
                     /translation="MKKAIGLSAILFGLLTACSAQGPSIADLPTAPESQRVDLAEPTF
                     SDPTAVTNPLFPIGELQRVILLGTVDGLPFRSETTLLAEAKPIEWNGRTVETLVSQYA
                     AFSDGRIHEVALDWYAQADDGSVWYLGEDVFNYEAGVVADTDGTWLAGREGPAAMIMP
                     GNPQVGDVYRPENAPGVVFEEVTVKSIDQTVHGPLGPVEGAIVTEELHMDGTTEGKIF
                     APGYGEFLTGAGGDLEAAALAVPLNALGGPVPDELASLSSAVRQILEASQVNDWSSAS
                     VSLDAVLAAWDSYRTGTLPRMLEAQMSSALVKLVAAVDAQQPPETGQAAIGVAWAALD
                     FELRHRPVSEIDLALFDLWAARVGVDAAAGDPANVAGDVATLEWIRDRFVHTLASADA
                     ERINALLADLRTVADAEDLAAAADIAAQLRDQPRGLLG"
     gene            complement(3166..4599)
                     /gene="merA"
                     /locus_tag="VI520_05505"
     CDS             complement(3166..4599)
                     /gene="merA"
                     /locus_tag="VI520_05505"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HLF37372.1"
                     /translation="MPEVESANMPRRDRFDLMVIGGGSAGFAAAIRGAELGYSVALVE
                     ADTIGGTCVNIGCIPSKTLIRAVEAHHQAGERRFRGIETHQGRIEWPQLLAHKDELVL
                     ELRKTKYTDVLAAYPDIAYIQGRARLTGKNGVDIDGRGYTTSKIIITTGAKPWAPPIP
                     GLSGVPYLDSTSALDLKELPESMIVLGANSVGLELAQLYSRAGTEVTLVETLPRIAPF
                     EDSAVSAALAEYLKFEGLLVVTRFDTQEVVKRNDRYHLTGSQAGAARTFEAGQLLVAT
                     GRRPNTAGMGLEEAGVSLGERGEILADDRMQTHNPFVYAAGDVIGKDMFVYVAAYGGM
                     LAAENALTGAGKAYDASYIPRLTFTDPQIASAGLTEEQARQQGYDVKVSTLPMDQVPH
                     AQAARDPRGLIKLVADAATDRLLGAHLLAPEAGEMIQTAVLAIRFGISVGELRETMFP
                     YLTSSEGLKLAAIAFEKDLAMLSCCAG"
     gene            complement(4611..5036)
                     /locus_tag="VI520_05510"
     CDS             complement(4611..5036)
                     /locus_tag="VI520_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLF37373.1"
                     /translation="MAMQIQDLAHRTGVSAKAIRYYESIDLLPSPKRRPNGYREYGQA
                     DVERLKLVAGARNLDFSLGDIEEILALRDRREAPCRVVLRLLEEKTGQIRERITQLQK
                     LEVQLRQLSAVGLTFPTDDVDGKNCVCHLVSIRAEASTA"
     gene            5469..>5842
                     /locus_tag="VI520_05515"
     CDS             5469..>5842
                     /locus_tag="VI520_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37374.1"
                     /translation="MLRLVFISAVIAGLTLVSAACSPKATSGSEVRVRPIDGILASGP
                     EFFDIGPETAALRVETSLPVVCAVVYGTTPEYGRMATDSDMAGGPHSDHHPLLTGLAP
                     DTLYYARLQGVGPDGTLYRSEE"
BASE COUNT         1009 a         1881 c         1834 g         1118 t
ORIGIN      
        1 aggtgctcac ccgtacccag attggcgagc acgtctggaa cttcgacttc tacaaccagt
       61 caaatgtagt ggatgtatac atcggctacc tgcggcgtaa gatcgatcaa ggaaacgaga
      121 ttcaactgat ccatactgtg cggggcgttg gctaccgcct cagcgaggag ggctgagccc
      181 ggcgtggtca ctcggttgcg gcccactcag ctcccgctcc gcctgcgcct caccctttgg
      241 tatctgctct ccctggggct catcttgctg ctcttcggcc tgctgctgta ttggcaagtg
      301 caacataact tactggtcca ggtggacagt gccctgcagc tggccgcggt ccagtcctcg
      361 gccacggtag gcatccagga tggtggcctg gactttcaaa atgccgatgc gtcgcccgcc
      421 ctcgcccggc tcagcgaaga cacggccatc cgcttgctgg ccgacgatgg aacggtgatg
      481 gacagcctcg gccgcgctgc cgaagcgcca gtcctgacgc cggcttccgg gtaccaaacc
      541 tatttcgacg gagacgacac ctggcgggtg tacggcgagc gggttgacct ccccgacgcc
      601 gcggccggcg gctggctgca agtcacccag tccctggagt cggtgcagca gactctgctg
      661 ctgcttcgcc ttcagttgct gtggggcgtg ccgctggccc tgctgctggc gggagccggc
      721 ggctactttc tagactctcg ggccatgcgc ccgatcgacc ggatcacccg caccgcgcag
      781 gccatcaact ccagcgacct ctccctacgc attggccact ccggtccggc cgacgagctg
      841 ggccggctgg cggcgacctt cgacgagatg ctcgaccgat tgcagtccgc cttcgaccgg
      901 gagcgccgct tcacggccga cgcagctcac gagctgcgca caccactcac tgccctgaaa
      961 ggccgcatcg gggtcacgct aagccagccg cgccaggcaa ccgagttttg cgattcgctg
     1021 caagaaatgg aacgtcaggt ggaccggctc atccggctga gcagcgacct gctgttcatc
     1081 gcccgactcg atcaggggca ggtcaaacgc cggcaggacc cgatcgcgct cgatgacttg
     1141 ctggcggcag tcgtcgagca gctccggccg ttggccgagg ccaaggcgat ttcactaccc
     1201 gaaccggatg ccagcgggct gaccgttccc ggagacatgg atctgatgat ccggttgttc
     1261 ttgaatctcc tggacaacgc cattaagttc actccaccgg gcggtcgagt gtcggttgag
     1321 gcacaacggt ccggcgagca agtcacgatc gccattcgag ataccggccc cggaattccc
     1381 gccgtgcatc gagcccatct cttcgagcgt ttcttccgga tgggcggcga ccgttcccgc
     1441 gcggacggtc aaggcggcgc cgggttgggc ttggccatcg cccacgagat tgcccgggcc
     1501 catggcggca gcctgacggt gaagagcgcg ctgggcgatg gaacgacatt cttcgtgaaa
     1561 ttgccgcgct ccccactgcc aagcgaggta ccggcgcttc gccattcagt ttcttaatct
     1621 catttcaccc gtttcttacc cttcagccct agcctcgggt cacttactgg agatctcgag
     1681 gtcgattgag ctagaggagc ttgagccatg atgaagaaag cgatcggact ctcagcaatt
     1741 ctgtttggac tcctgaccgc ctgttcggcg caaggtccga gcatcgctga cttgccgacc
     1801 gcgccggaga gccaacgagt cgatctggcg gagccgacct tctccgatcc gaccgcggtt
     1861 accaatccgc tcttcccgat cggggagctc cagcgggtga tcctgctcgg caccgtggat
     1921 ggcctcccgt tccggtccga gacgacgttg ctcgccgagg ccaagcccat cgagtggaac
     1981 ggccggaccg tcgagacgct ggtctcgcag tacgccgcgt tttcggacgg gcggatccac
     2041 gaggtggcgc tcgactggta cgcgcaggcc gacgacgggt cggtctggta cctgggcgaa
     2101 gacgtgttca actacgaagc cggggtggtc gccgataccg atggaacctg gctggccggg
     2161 cgggaaggcc cggccgccat gatcatgccc gggaatccgc aggtcgggga tgtgtatcgg
     2221 ccggagaacg cccccggagt ggtcttcgag gaggtcactg tcaaatcgat cgaccaaacc
     2281 gtccacggtc cgctgggtcc agtcgagggc gcgatcgtca cggaggaact gcacatggac
     2341 gggaccaccg agggcaagat cttcgcaccg ggctacggcg aattcctgac cggcgcggga
     2401 ggcgacctgg aagccgcggc gttggcggta ccgctcaacg ccctgggcgg accggtgccg
     2461 gacgagctgg cctcactctc gtccgcggtc cgacagatcc tggaagcctc ccaggtcaac
     2521 gactggagca gtgcctccgt cagcctggat gcggtcttgg ctgcctggga ctcgtatcgg
     2581 accggcactc tacccaggat gctggaggcc cagatgagtt cagcgctggt caagttggtg
     2641 gcggcggtcg acgctcagca gccccccgaa accggccagg cggccatcgg tgtggcatgg
     2701 gccgcgttgg atttcgagct gcggcaccgg ccggtctccg agatcgatct ggctctgttc
     2761 gacctgtggg ccgctcgggt gggcgtggac gccgcagccg gcgatccggc gaatgtggcc
     2821 ggagacgtcg ccaccttgga gtggatccgg gaccgcttcg tccacaccct cgcctccgcc
     2881 gacgccgagc gtatcaatgc cttactcgct gacttacgga ccgtcgcaga cgccgaagat
     2941 ctcgccgccg cggctgacat cgcagcccag ctgcgggatc agccccgtgg actgctgggg
     3001 tgagcttcag ggcttgtaga cccgggtctc cggtttgaag gcgacccaag aaaaccagaa
     3061 gtgattgatc gcgacaaccg gggtcagacg ctcgccgtgt ggcctgccca acgcctcgag
     3121 cgctgagttc aatcgcccga cctggcgcag ccgatcttct tcaatctacc ctgcgcagca
     3181 gctcaacatc gccaggtctt tctcgaaggc gatcgcggct agtttcaacc cctcgctgct
     3241 ggtcaggtaa gggaacatgg tttccctcag ctcgcctacg ctgatcccga agcggatggc
     3301 cagcaccgcg gtctggatca tctcgccggc ctccggcgcc aagaggtgcg cgccgagcag
     3361 tcgatcggtc gcggcgtcgg cgacgagctt gatcagccct cgagggtcac gcgcagcctg
     3421 ggcgtggggc acctggtcca tcgggagggt gcttactttc acatcgtagc cctgctggcg
     3481 cgcctgctct tcggtcaacc cggcggaagc gatctgcggg tcggtgaagg tgaggcgcgg
     3541 aatgtaggag gcgtcgtagg ccttccctgc gcccgtcagc gcgttctcgg ctgcgagcat
     3601 gccgccgtaa gccgcgacgt agacgaacat gtccttcccg atcacgtctc cggcggcgta
     3661 cacgaagggg ttgtgcgttt gcatccgatc gtcagccagg atctcgccac gctcgccgag
     3721 gctcaccccc gcctcttcca gccccatgcc ggcagtattc gggcggcggc cggtggcaac
     3781 cagcagttgc ccggcctcga aggtccgggc ggcgccggct tgagacccgg tcaggtggta
     3841 gcggtcattg cgtttcacca cttcctgagt atcgaatctc gtgacgacca gcagaccttc
     3901 gaatttaaga tattccgcca acgccgcgct gaccgcggag tcctcaaacg gtgcgatgcg
     3961 cggcagggtt tcgaccagcg tcacttcggt cccggcgcgg gaatagagct gggccagttc
     4021 caggcctacc gagttcgcgc cgaggacgat cattgattcc gggagttctt tcaaatcgag
     4081 cgcggaggtg ctgtccaaat aggggacccc ggagagtccg gggatgggcg gggcccaggg
     4141 cttcgccccg gtggtaatga tgatcttgct ggtagtgtag cctctgccgt caatgtccac
     4201 tccattcttg ccggtcagtc gagcccggcc ctggatgtag gcgatgtctg gataggcagc
     4261 caacacatcg gtgtacttcg tcttgcgaag ctccaacacc agctcgtctt tgtgagcaag
     4321 cagctgcggc cactcgatcc ggccctggtg cgtctcgatc ccgcggaacc gtcgctcccc
     4381 agcctggtga tgagcctcga cggcgcggat gagcgtctta gaaggaatgc agccaatatt
     4441 gacgcacgtt ccgccgatcg tgtcagcttc gactaaggcg accgaatacc caagctccgc
     4501 accccggatg gccgcggcga accccgccga cccgcctccg atcaccatga ggtcgaaccg
     4561 atccctcctc ggcatgttgg cagactcgac ttcaggcatg tcctgctccc ttaagctgtc
     4621 gaagcctctg cacgtatact gaccaggtgg cacacgcagt tcttaccgtc aacgtcgtcg
     4681 gtagggaagg tcaggccgac tgcggacagc tgacgcaact gcacttccag cttctgaagc
     4741 tgcgtgatgc gctcccgaat ttggccggtc ttctcttcca acaaacgtag gacgactcga
     4801 cagggcgcct cgcgccgatc gcgtagcgcc aggatttcct cgatgtcgcc gagtgagaaa
     4861 tcgagattgc gcgcgccggc caccagcttc agccgctcga catcggcctg gccgtactca
     4921 cggtagccgt tgggcctgcg tttgggcgac ggcagcaagt cgatggattc gtagtagcga
     4981 attgccttcg ccgagacgcc cgtacgatgg gctaagtcct ggatctgcat tgccatccct
     5041 catacttact tccgtgtaac cataggatac accttccagt aactggaagg tcaaggatcg
     5101 aatctctctc aaaccatcga tcgacttcga tgtccaactg ccgatagagc tccggcggga
     5161 caccgatttc ggtgaggtac aggttaccta catagggcct agcttgtggc gccagcaacc
     5221 cggtctttgg caggacgagc gtgagagtgg ccgtggcggg cggggatctc gccggtcgcc
     5281 ctatgaatgc attcttactt cgcagccgca tgatctacga tgatcggggc gtgatatacg
     5341 gactctctga aagaccgtgt gctatcctca atcccgaccc aggctgcctg tccattctgg
     5401 tgccgcacac gccgctgatc atgagtgaat atcgtgttgg caatctagcg cctgtggagg
     5461 agggatcgat gctgcgtctg gttttcattt ctgcggtgat tgcgggtttg acgcttgtct
     5521 cggcggcctg cagtcctaag gccacttcag gctcagaggt tcgagttcgc ccgatcgacg
     5581 gtatcctggc ctctggcccc gaattcttcg acatcggtcc cgaaaccgcc gcgctgcgag
     5641 tcgagaccag cttgccggtg gtgtgcgcgg tcgtctacgg cactactccc gagtatgggc
     5701 ggatggcgac cgacagcgat atggcggggg gtccacattc cgatcatcac cccctgctca
     5761 ccggcctggc gcccgacacg ctgtactacg cccgactgca aggggtgggc ccggacggca
     5821 cgctgtaccg cagcgaggag ta
//
LOCUS       DATDPQ010000247         9269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_377177, whole genome shotgun sequence.
ACCESSION   DATDPQ010000247 DATDPQ010000000
VERSION     DATDPQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377177"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="VI520_05520"
     CDS             <1..2022
                     /locus_tag="VI520_05520"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HLF37375.1"
                     /translation="SLVKSSAGVATGPVGFLRVYDKAFGEIAQGGTRRGANMAVLRVD
                     HPDVREFITCKVDENQITNFNISVGATDAFMRAVRDDTTYDLVAPHTGEVVETVRARE
                     IFDLMLEHAHHNGEPGILFLDAANRSNPVPHLYELEATNPCGEQWLGPYENCCLGSIN
                     LAEHCGPSGAVDWAALEHSIVVSTRFLDDVVSANAYVPAVPNLRIAADRARRIGLGFM
                     GLGDLMYNVGVRYGSEEAQEFAAQVMEFVRYHSMRTSIELAGQRGPFEAITGSMYDPA
                     DLKWSPPTPIASYRRHFGRPDLDWSTITDGIRQHGIRNAAQTTIAPTGTIATVAGCEG
                     YGCEPVFALAYVRHVDDQGQDLKLTYASPLFEKALVEAGVDDSTHVRIIEQVMERGSC
                     QDIADVPEHVRHAFVVSQDITAEEHVRMQAAMQAFVDNSLSKTCNFPEDATVADVGQA
                     YLLAWELGCKGLTVYVTGSRQKVVLETRSTAEKKTAGSGDTAAALVQAVSEAQLQMWM
                     PGKKPRPKRLQGSTFSANTPVGKAFITVNENGGKQPFEVFITTAKAGSETAAVSEAIG
                     RLISYILRLASPVMPRERLVEVVRQLSGIGGGRPTGFGAERVLSLPDGVAQTLGTYLS
                     DTEEQFTAAASAREPLPMPIGDLCPDCGAAAMVSEEGCRKCYSCGFSEC"
     gene            2493..3221
                     /locus_tag="VI520_05525"
     CDS             2493..3221
                     /locus_tag="VI520_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37376.1"
                     /translation="MAGEIVPSEMSFAQWIEEVDLGDLADEAGELAEVAPYLKYVVGA
                     YRWYRSFRARQFLRSLSSATQSFTSEQRQKFEQVIKSDIGSELLADYAEKVLRTSSRT
                     AIAALALLYADVDDVTYSREFKRATMIALEGISEDRIDAFVELSQVDEFIPDKNLPNV
                     PYPVAVANDKLIAALSPEARELLESPELRITIISDLIRRGLLMPDFASARLGDGGVGM
                     TFGITQQTARLRSVILHARQLLSE"
     gene            3353..4261
                     /locus_tag="VI520_05530"
     CDS             3353..4261
                     /locus_tag="VI520_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37377.1"
                     /translation="MDSVIREIRSNIETVERQQVLYEVTNFSDRAEALDFLESHVIDR
                     IEGLLLQDGWSEPLSGLMRYAEKVRLGLEGVDQGLFRRLREDIASGNCTSSDLRNRIT
                     MYARRASSGGTHSAEGYDSLDALVSGLFLTEVAPEVPSQRDPEMVAYQPAPARVTLEL
                     VERADFRQHDVFYDIGSGLGLVPILVHLLTGVRARGVEVEPAYCEYARRRASALRLSE
                     VQFFNVDARKADYSDGTVFFLYSPFEGNMLEQVLEGLRHESTTRSIRLCTYGPCTLQA
                     TQQKWLEPLDQNGNHVNRLAIFATRQ"
     gene            4399..4728
                     /locus_tag="VI520_05535"
     CDS             4399..4728
                     /locus_tag="VI520_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HLF37378.1"
                     /translation="MASAYRHLQEAWARLCPIFQGKDGPLILRRIREQSAQEIQTNLR
                     AAFGEADSRMPLDLLANYLAGAQIALMQWWLAKRRPHSPEILAQTLHRLQRAAIRDAF
                     ELGDAEQ"
     gene            complement(4748..4933)
                     /locus_tag="VI520_05540"
     CDS             complement(4748..4933)
                     /locus_tag="VI520_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37379.1"
                     /translation="MNYVLLALVIAHAFFYGAVVRADSLYTLLLLLTVIAVFVGQMMG
                     IWLWRRGHRQSPARPAG"
     gene            5048..5998
                     /locus_tag="VI520_05545"
     CDS             5048..5998
                     /locus_tag="VI520_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37380.1"
                     /translation="MRQRMLNDFRVLLSVALAALGTSAMAWADIGPKPSMDFQFVYQD
                     LPGLHIQSGTLLECADPACVNAEALREVGPQRFTCNLDSCSSVAYSYQDYYKLAITFS
                     DGVTRESNVFGTDHYGGFYRVTVSEGGLLVTEVGGGANLLSALSFIGAAAALLFVGSM
                     LGLVTVLAALSVGARKRPLSLESRRGWLVVGWALAVPVVVLTSLASPAVAVTVVLELG
                     LGFLYATLRRKPRFTLVTTLLLVNALTSPLLGIAWSILRYVTVGTPGLWQGFAELVVW
                     LVEAAAIYFTHRSAFSFREALALAFGLNATSWLVGLVLPL"
     gene            6209..7528
                     /locus_tag="VI520_05550"
     CDS             6209..7528
                     /locus_tag="VI520_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37381.1"
                     /translation="MRVGLKAIRPVFRTSSALVALLVAACSLAPTAMTIATVTPEPPN
                     TAAPTETPAPTPSPVQFVAPPPPPPPTALSVSGPLTINIIQMIDRNTGWGQGVLGNGQ
                     ATRILRTSDGGVTWQDVSPGLLDNFGRPAFFLDAQTAWAWAWEGSERWRTQDGGQKWS
                     PVEGMSWGDELWFNNGLHGWRAHLGGWGWMSGFLSLGFDYLAITEDGAQTWQEVNPPP
                     GNSHTYLALPDSQTAWALQMESVFREWGGFQYLKVPWSVLVTRDAGRTWQAETVPIPP
                     DARLASYSEPGLYLDEAGLCEFVSPAYSSSTVWKFALTCEDQGWLYTTANQGQTWAIN
                     PLPQGALIRGLYFLNSSVGWILRWDELGSGLGRLYLTTDGGENWTLLRHTEWALAQFD
                     FVDAGTGWAVASACTEFYCDRFEDPSALVKTTDGGLTWQMLEPRLRP"
     gene            7584..9026
                     /locus_tag="VI520_05555"
     CDS             7584..9026
                     /locus_tag="VI520_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37382.1"
                     /translation="MNLHSARPALGFLVLIALTACDLSSGLNRDLDATTPSSTVPALL
                     VTTPVPVATISPEPASSTPEPVSPTPRKPSPTPRPAAIDLSSFSDVRRMGRLSLTQGW
                     VLTDSFNGECGADPSCRARLMLTEGLIPMERLLWTEDAGASWSDITPAGLSNCPPPDI
                     CTMVSPPVFLDSSRVRIAVLRGQELEPPTTLSFMYTENGGRTWGLWHIDELHYGRICP
                     GEACISDADLEFSDPLNGWLAASAPLGMGTDTIYLYRTRDGGQSWTALVKVSVSDTGG
                     PGFVPKPIVHHLTFIDASTGWEVGGWRFDTGQLLVTRNGGSSWQPVELLIPEAYVGMS
                     RAYDAPVFFSHDSGVLPVRFYGREDGDQVLGFYITGNAGGTWLLTATLEDPELNTSSD
                     VRGIAWSAIDEATWFVAVSETRQYLTRDRGLTWEVFAAEGLDGAALIDVQFVSEVEGW
                     GLGRTDFDYVPRMFATHDGGRTWMPIAPSP"
BASE COUNT         1717 a         2882 c         2927 g         1743 t
ORIGIN      
        1 tcgctcgtga aatcgtcggc cggggtcgcc accggcccgg tcgggttcct gcgggtgtac
       61 gacaaggcct tcggcgagat cgcccagggc ggcacccgcc gcggcgccaa catggccgtg
      121 ctgcgggtgg accatcccga cgtccgtgag ttcatcacct gcaaggtcga cgagaaccag
      181 atcaccaact tcaacatctc ggtcggcgcg accgacgcct tcatgcgggc ggtgcgcgac
      241 gacaccacct acgacctggt cgcgccgcac accggcgagg tggtcgagac cgtgcgggcc
      301 cgcgagatct tcgacttgat gctcgagcac gcccaccaca acggcgaacc gggcatcctg
      361 ttcctggacg ccgccaaccg cagcaacccg gtcccgcact tatatgagct ggaagcgacc
      421 aacccctgcg gcgagcagtg gctgggcccc tacgaaaact gctgcctggg ctcgatcaac
      481 ctggccgagc actgcggccc gagcggggcg gtcgattggg cggcgctcga gcacagcatc
      541 gtcgtgagca cccgtttcct ggacgatgtc gtgagcgcca acgcctacgt gccggccgtg
      601 ccgaatctgc ggatcgcggc cgaccgggcc cgccggatcg gcctggggtt catggggctg
      661 ggcgacctga tgtacaacgt cggcgtgcgc tacggctccg aggaagccca ggagttcgcc
      721 gcccaggtaa tggagttcgt gcgctaccat tccatgcgga ccagcatcga gctcgccggg
      781 cagcgcggcc cgttcgaggc gatcaccgga agcatgtacg atccggccga cctcaagtgg
      841 tcgccgccga ctccgatcgc ctcctaccgg cggcattttg gccggccgga tctggattgg
      901 tcaacgatta ccgacgggat ccgccagcac ggcatccgca acgcggccca aaccaccatc
      961 gcgccgaccg gcaccattgc gaccgtcgcc ggctgtgagg gctacggctg cgagccggtg
     1021 ttcgcgctgg cgtatgtccg gcacgtggac gaccagggcc aggatctgaa gctgacctat
     1081 gccagcccgc tgttcgagaa ggcgctggtc gaggctggcg tggacgactc gactcatgtg
     1141 cggatcatcg agcaggtcat ggagcggggg agctgccagg acattgccga cgtgccggag
     1201 cacgtccggc acgccttcgt cgtctcgcag gacatcaccg ccgaggagca cgtgcggatg
     1261 caggccgcca tgcaggcgtt tgtggacaac agcctgagca aaacctgcaa tttcccggag
     1321 gatgccaccg tggccgacgt gggccaggcc tacctgttgg cctgggagct gggctgcaag
     1381 gggctgaccg tgtacgtgac cggctcgcga cagaaggttg tgctggagac ccgctcgacg
     1441 gccgagaaga aaacggcggg cagtggcgac actgcggccg cgctagttca ggcggtcagc
     1501 gaggcgcagc tgcagatgtg gatgccaggc aagaagccgc ggccgaagcg gttgcagggc
     1561 tcgaccttca gcgccaacac tccggtcggc aaggccttca tcaccgtcaa cgagaacggc
     1621 ggcaagcagc cgttcgaggt cttcattacg accgccaagg ccggctccga gaccgccgcg
     1681 gtgtccgagg ccatcggccg gctgatctcg tacattttgc ggctcgcctc gccggtgatg
     1741 ccgcgggagc ggttggtgga ggtcgtgcgc cagctctccg gcatcggcgg cggccgcccg
     1801 accggcttcg gcgccgagcg ggtgctctcg ctgcccgacg gcgtggccca gaccctgggc
     1861 acttacctga gcgacaccga ggaacagttc accgccgccg catccgcccg cgagccgctg
     1921 cccatgccga tcggcgacct gtgccccgac tgcggcgcgg cggccatggt gtccgaagaa
     1981 ggctgccgga agtgctactc ctgcgggttc tcggagtgtt agacacctga actagactct
     2041 agcgcactgt cgactttggg agggggatgg aatggaatga cggactactt ccctgccttg
     2101 tatacagctg gtcctcgcag cccggctgtt tgagcctacc gaactatacc aacccatcag
     2161 ccggcgatta ggccgaggtc tggaaccagt gcggtcagca accccttggc gtggcctgcc
     2221 ccaggggtac catgattcga gtaccgcccc tggccaatca ttccgaccgg caccaagacg
     2281 ctaaggtggt tcagcatttg ttttgaccgc gcgagggatc ttagctacac atatgagcgc
     2341 gattccgacg ataaacgaga aatccacccc gataaacccg cgaatgggac caaaagcgga
     2401 aatacttcct cgatagagcg gtgcaggggc gaaatgagga agcacttccg cataatcact
     2461 agttaggcag agatcacctg gaggaagcaa tgatggctgg tgaaatcgtc ccgagtgaaa
     2521 tgagctttgc acaatggatc gaggaagtag acctcggtga tcttgccgat gaggcggggg
     2581 aacttgcgga agtcgctccg tacctgaaat acgttgttgg ggcataccga tggtatcgga
     2641 gcttcagggc aaggcaattc cttcggtccc tttctagcgc gacccaatct ttcaccagcg
     2701 agcagcgcca gaagttcgaa caggtcatca agagcgatat cggctccgaa ttgctcgccg
     2761 attatgctga gaaggtcctg cgaacttcgt cccgcacggc gatagcagca cttgctcttc
     2821 tgtacgcaga cgtggacgat gtcacgtatt ctcgcgaatt caagcgtgcc accatgatcg
     2881 cacttgaagg aataagtgag gatcgcatcg atgcgtttgt cgaactatcc caagtcgatg
     2941 agttcatccc agacaagaac ctccctaacg tcccgtatcc ggtcgcggtc gcaaacgaca
     3001 aactcattgc ggcactctct ccagaagctc gggagctgct tgaatcccct gagcttagga
     3061 tcacgatcat cagcgacctg attcggagag gcctcttgat gccagacttc gcttcggcca
     3121 ggttgggtga cgggggcgtt ggcatgacct ttggaatcac acagcaaacc gccagactga
     3181 ggagtgtgat tctgcatgcg cgccaactac tctcagaata gcgacccctt atctctgcct
     3241 aaccactcgc tggagcggac gcagcctcag cgcgttttca tgaatgatgc agctatgctg
     3301 cgccgctcag ctcgaggccg ttaggcagct ccacaaggga ggatcgagca agttggatag
     3361 cgtgatccgt gaaatccgat cgaacatcga aaccgtggaa cgccaacagg tactgtatga
     3421 ggtgacgaac tttagcgaca gggcagaggc cctggatttc ctcgagtccc acgttatcga
     3481 tcggattgag ggcctgctcc tccaggacgg ttggtcggag ccactatcag gcttgatgcg
     3541 atacgcagag aaggtgagat tgggattgga aggggtcgat cagggccttt tccgaaggct
     3601 tcgggaggac attgcctccg gtaactgcac cagttcggat ttgaggaacc gaattacgat
     3661 gtacgctcgc cgtgcctcaa gtggtggaac tcactcagct gagggatacg atagtctaga
     3721 tgcgctagtc agcggtctat tcttgactga agtagccccc gaagtcccca gccagcgaga
     3781 cccggagatg gtcgcctatc agccggcccc agccagggtc actttggagc tcgtggaaag
     3841 agccgacttc cggcagcacg acgtcttcta cgacataggt tctggcttgg ggttagtccc
     3901 aattctggtg caccttctca ccggagttcg ggcaaggggc gtggaggtgg aacccgccta
     3961 ttgcgaatat gcgaggcggc gcgcaagcgc actacgtctc tcagaggtgc agttcttcaa
     4021 cgtggatgca cgcaaggcgg actactcgga tgggacggtc ttcttcctct attcgccttt
     4081 tgaaggcaac atgctggagc aagtgttgga gggactgagg cacgagtcaa cgacgaggag
     4141 catccgattg tgcacatacg ggccctgtac gctgcaggcg acgcagcaga agtggctcga
     4201 acccctcgat cagaacggca atcacgtgaa tcggctagcc atctttgcta cgcgccaata
     4261 aggacagaag ctgcctaacc cctcgctgga gcggacccgg ccggctcggc ccgataatct
     4321 gaacgagtct tggccgggcc gctttacccc cacccactct cccgggaaga tctgttgtcg
     4381 cctgaaccgc cagcggggat ggcctcagcc taccggcatc ttcaagaggc gtgggcgcgg
     4441 ctctgtccca tttttcaggg caaggatggg ccgctgatcc tgcgccgcat ccgtgagcag
     4501 agcgcgcagg aaatccagac caacctgcgc gccgccttcg gtgaagccga cagccgtatg
     4561 ccgctcgacc tgctggcgaa ctatctagcg ggggcgcaga tcgcgctcat gcaatggtgg
     4621 ctggcgaagc gccggcccca ctcgcccgaa atcctggccc agacactgca ccggcttcag
     4681 cgcgcggcga ttcgcgatgc attcgagcta ggggatgctg agcagtgact ctcgagcgcc
     4741 tctcgcccta acctgctggg cgagcggggc tctgccggtg ccctcgccgc cacaaccaga
     4801 tgcccatcat ctgtccgacg aagaccgcga tcacggtgag caggagcagc agcgtgtaga
     4861 gggagtcggc acgaacgact gcaccgtaaa agaatgcgtg cgcgatgacc agagccaaca
     4921 agacgtagtt caagcgctgc agccgcttcc aggttctagc ccgcccggca acagaatggt
     4981 tggatgctca tcgcagctcg gaccgccaat tgcatgcaat gcgagcatct gagagaatcc
     5041 gaggatggtg agacaacgca tgcttaacga ctttcgggtt ctgctatccg ttgcgcttgc
     5101 ggcgctggga acatcggcga tggcctgggc cgatatcggg ccgaagccat ccatggactt
     5161 ccagttcgtc taccaagacc tccctgggtt acatatccag agtggaacgc tcctggaatg
     5221 tgccgatccg gcctgcgtta acgccgaagc cctccgggag gttggccccc agcggttcac
     5281 ctgcaacctc gattcctgct cttctgttgc ctacagctac caggactatt acaagttggc
     5341 gatcaccttc tccgatggag tgacccgtga aagcaatgtc tttggcacgg accattacgg
     5401 ggggttctat cgtgtcactg tctccgaggg gggcctgttg gtaactgagg tgggcggggg
     5461 agcgaatttg ctttcggccc tgtcattcat cggggcagcg gctgccctcc tgttcgtcgg
     5521 gagcatgctt ggtctggtaa cggtcctggc cgcgttgtcg gttggggcga gaaagcgccc
     5581 gctcagtctc gagagccgcc gcggttggct cgttgtgggt tgggccctgg ccgtgccggt
     5641 ggtggtcctg acctcgttag cttctccggc cgtcgccgtg acggtggtgc tcgagcttgg
     5701 gctgggattc ttgtatgcca cgctccggcg caaacctcgg ttcacactag tcacaaccct
     5761 cctgctggtc aacgccttga cctcgccgct tctgggcatc gcatggagca tattgagata
     5821 cgtcacggtc gggacgcctg gtctatggca ggggtttgcc gaactcgttg tctggttggt
     5881 ggaggcggcc gcgatctatt tcacacaccg cagcgctttc tcattcagag aagctctggc
     5941 gttggcattc ggcctgaacg cgaccagctg gctggtagga ttggtgctcc cactctgacg
     6001 ctggacggca cgcttgactc gccgagctgg tcgccggggc catagccgaa tgctgggcgg
     6061 cctacggaca ccggcttcgg gactgcgttc cagcccgata gggcacatct cggtaatcag
     6121 gcggtaactc tgcctggaac ctgcggagag cttcgccggc tacaatcccg atcaagatcg
     6181 atccagaccc tactcgggag aagccccaat gcgcgtcggc ttgaaggcca tccgcccagt
     6241 cttccggacc tcatcggccc tggtcgcact gctggtcgcg gcctgtagtc tggcgcccac
     6301 agcaatgacg attgcgacgg tgacccccga gccgcccaac accgcggccc cgacagagac
     6361 accagctccg acacccagcc ctgtgcaatt cgttgcccca cccccgcctc cgccgccaac
     6421 cgcgctctcg gtctccgggc cactgaccat caacatcatt cagatgatcg acagaaacac
     6481 aggttggggg cagggggtcc tgggcaacgg gcaagccacc cgcatccttc gcaccagcga
     6541 tggcggagtg acctggcagg acgtcagccc ggggctgctc gataacttcg gccggcctgc
     6601 gttctttctc gatgcacaga cagcctgggc ttgggcttgg gagggcagtg agaggtggcg
     6661 cacccaggat ggtggtcaga aatggagtcc cgtcgagggc atgagttggg gggacgagct
     6721 ctggttcaac aacggcctgc atggctggcg tgcccatctg ggtggttggg gttggatgtc
     6781 gggctttctc tcgcttggat tcgattacct cgcgatcaca gaggacgggg cgcagacttg
     6841 gcaagaagtg aaccctcctc cggggaacag tcatacgtac ctggcgctcc cggactcgca
     6901 aacggcctgg gcactccaga tggaatccgt cttccgcgag tgggggggct ttcagtatct
     6961 caaggttcca tggtctgtcc tggtcacccg cgatgctgga cggacgtggc aagcggagac
     7021 tgtgccgatt ccgccggatg cccgccttgc tagctactcc gagcctgggc tctatttgga
     7081 cgaggccggc ctgtgcgaat tcgtatcacc ggcctattct tcaagcacgg tttggaagtt
     7141 tgccctgacc tgcgaggacc agggctggct gtacacgact gccaatcagg gccagacctg
     7201 ggccatcaac cccctgccgc agggtgccct gatccgcggt ctctacttcc tcaactcgag
     7261 cgtgggctgg atacttcgct gggacgagct cggcagtggc ctgggccggt tgtacctgac
     7321 caccgatggc ggcgagaact ggacgctgct caggcacacc gagtgggcgc tggcccaatt
     7381 cgatttcgtg gacgcaggaa ccggctgggc cgtggcctcg gcttgcaccg aattctactg
     7441 tgatcgtttt gaggacccca gtgcgctggt caagaccacc gacggcggcc tgacctggca
     7501 gatgctcgag ccgcggctga ggccatgaac ggcgatctgg ctccctctcg gccgtccctc
     7561 ggacgagcat tggaggtggt tctgtgaatt tgcattcggc acgcccggct cttggcttcc
     7621 tagtcctgat cgcgctcacc gcctgcgatc tctcatcggg cctcaaccga gacctcgacg
     7681 ccacaacccc gagctcgacc gtaccggctc tgctggtgac gacgccggta ccggtcgcca
     7741 cgatctcgcc cgaaccggct agctcaacgc ccgaaccggt cagcccgacg cccaggaagc
     7801 ccagccccac accgcggccc gcggcgatag atctgtcgtc gttctccgac gtgcgtcgga
     7861 tgggccggct ctcgctcaca cagggctggg tcttgacgga cagttttaat ggggagtgtg
     7921 gagcggaccc cagctgccgg gctcgcctca tgctcaccga aggcttgatc ccgatggaga
     7981 gactgctgtg gaccgaagac gcgggggcca gctggtctga catcacccca gcggggcttt
     8041 cgaattgtcc accgcccgat atctgcacga tggtctctcc gccagtcttt ctcgactctt
     8101 ccagggtgcg gatcgcagtc ctccggggac aggagctgga gcccccaacc accctctcgt
     8161 tcatgtacac cgagaatggc ggaaggacct ggggcctatg gcacatcgat gaactccatt
     8221 acggccgcat ctgtccagga gaggcctgca tcagcgacgc agacctcgag ttctccgacc
     8281 cactcaatgg ctggctggcg gcgtccgccc cgttgggcat gggtaccgat accatctatc
     8341 tgtaccgcac ccgggacggc ggacagtcgt ggaccgcgct ggtcaaggtc agcgtcagcg
     8401 acaccggggg tccagggttc gtgccgaagc cgatcgtcca ccacctcact ttcatcgatg
     8461 cgtcgactgg gtgggaagtt ggcggatggc gtttcgatac cggtcaactg ctggtcacgc
     8521 ggaacggagg cagcagttgg cagcccgtgg agctcctcat cccggaggcg tatgtcggca
     8581 tgagccgtgc ctacgatgcc cccgtgttct tctcccacga ctctggggtg ctgccggttc
     8641 ggttctacgg gcgcgaggac ggggaccagg tgctggggtt ctacatcacc ggcaacgccg
     8701 gtgggacttg gctgctcaca gccacattgg aggacccgga gttgaacaca tccagcgatg
     8761 tccgcggcat cgcctggtcg gccatcgacg aagcgacctg gttcgtcgcc gtcagtgaga
     8821 ccaggcagta cctcacccgc gaccgcgggc tgacctggga agttttcgcg gccgaagggc
     8881 tagacggcgc ggcactgatc gacgtgcagt tcgtttccga ggtggagggc tggggactcg
     8941 gcaggaccga cttcgactat gtgcctcgga tgttcgccac gcacgacggc ggtcgcacct
     9001 ggatgccaat tgcaccttcg ccgtaggcac gggaatcaca tcggtggcgg atgaggcctt
     9061 gccgcctaac ctgtgtccct cggaattcgc cgtgcccaac gcggagctcg gccggctgct
     9121 cgataggggc agtcccgcct tgttacttga ctccggtccg agaccgcagc acgtgttcca
     9181 ggacgaacgt ggactgggtg aactcttgaa gcgacagccg ctcttccggg gtgtggtcga
     9241 gcgctgaatc acctgggccg tagccgacc
//
LOCUS       DATDPQ010000248         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1949151, whole genome shotgun sequence.
ACCESSION   DATDPQ010000248 DATDPQ010000000
VERSION     DATDPQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949151"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /gene="argS"
                     /locus_tag="VI520_05560"
     CDS             complement(<1..1134)
                     /gene="argS"
                     /locus_tag="VI520_05560"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLF37383.1"
                     /translation="MFDSEIRELSAQVRAAFSRAGIPQPKDIQWAPTPFEGQWGIGTA
                     VCYQTAAREAKKTPGLKVPARAREIAELVAGEFETPPGFERIEAQNAYLNAYFDTGVF
                     AARVVDEAIGRRAEFGKGEPKPERVMVEYAQPNTHHSFHIGHARNVVLGESLARLTEF
                     AGYPTIRAAYPGDVGLGVMTCVWGYQKFHYGEEPQGVHERGQWLAGVYTEANSLVEPA
                     EQETPEQRARREQYDLERRELYRRWDSGDPEVHRLWLMTRKWSLDELEDILRILGAPI
                     EVFFFESEVDEAAKRIVGELVEAGVADDERPDGPVIVKIDQKLGLTKEKFRTVVLLRS
                     DGTTLYSAKDLALAKRKFKEYDVDRSIYVVDVRQSLHFQQVFKI"
     gene            complement(1225..1500)
                     /gene="rpsT"
                     /locus_tag="VI520_05565"
     CDS             complement(1225..1500)
                     /gene="rpsT"
                     /locus_tag="VI520_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLF37384.1"
                     /translation="MANTRSAKKRIRQNEKRRERNRPVRTRARTQVRIARTAIAAGKQ
                     DQAAAAVKEAIQQLDRAAEAGVIHRKNAARRKSRLMKSLAAVQQDRG"
     gene            complement(1592..>2085)
                     /locus_tag="VI520_05570"
     CDS             complement(1592..>2085)
                     /locus_tag="VI520_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HLF37385.1"
                     /translation="APAAAVGRMVHAAIQRSAMPGSAGYEPFMENEAFGAGLVDQRQR
                     AAAVAESTEMLGRLAAHALWEEIGPAEAAQREIPFTTSDPADQVQSGQIDLLWRGEQG
                     WKIVDFKTDALRSEADLPAAVERHRKQVERYVRSAASILGEKPRGMLCFLDAVGAVQV
                     VEL"
BASE COUNT          339 a          697 c          613 g          436 t
ORIGIN      
        1 gatcttgaat acctgctgaa agtgcagact ctgtctgacg tctaccacat agatcgagcg
       61 gtccacgtcg tattccttga acttgcgttt cgccagcgcc aggtccttcg ccgagtagag
      121 ggtcgtcccg tcgctgcgca gtaggactac cgtccgaaac ttctccttgg tcaggccgag
      181 tttctggtcg atcttgacga tcactggccc gtccggccgt tcatcatctg ccacccctgc
      241 ctcgaccagc tcgccgacga ttcgtttggc ggcttcgtcc acttcactct cgaagaaaaa
      301 gacttcaatg ggagctccca ggattctcaa gatgtcttcg agttcgtcca aactccattt
      361 gcgggtcatc agccacagcc ggtgaacttc cgggtcgccg ctgtcccaac ggcggtacag
      421 ctcgcggcgt tctaggtcgt actgctcgcg gcgggcgcgc tgctctgggg tctcctgttc
      481 cgccggttcg accagtgaat tggcttcggt atataccccg gcgagccact ggccgcgctc
      541 atgcacgccc tgcggctcct cgccatagtg aaacttctgg tagccccaca cgcaggtcat
      601 cacgcccagg ccgacgtcgc ccggataggc cgcccgaatc gttggatagc cggcaaattc
      661 ggtcagccgg gccagcgact ccccgagcac gacgttccgg gcatggccaa tgtgaaatga
      721 atgatgggtg ttgggttggg cgtactcgac cattacccgc tcgggtttcg gctcgccttt
      781 cccaaattcg gctctccgcc cgatggcttc gtccactacc cgggcggcaa aaacgcccgt
      841 gtcgaagtac gcgttgaggt aggcgttctg tgcctcaatc cgctcaaatc cgggaggcgt
      901 ttcaaactcc ccggcgacca actcggcaat ctcccgggcg cgggccggga ccttgagtcc
      961 cggggtcttt ttggcctcgc gggcagccgt ctgatagcag accgcggtgc cgatgcccca
     1021 ttgtccttcg aacggagtgg gcgcccactg gatgtctttg ggctggggaa ttccggctcg
     1081 actaaaggct gcccgcacct gtgcgctgag ctcgcggatc tccgagtcga acatggctta
     1141 aggcccggcg actatactga gcgagaaaaa caaaagacct cgaccgttgt agtcgaggcc
     1201 tgaggtgatg gcaggctgca ctctttaacc gcggtcctgc tggacagcgg ccaagctctt
     1261 catcaggcgg ctcttgcggc gggccgcgtt ctttcgatgg atcactccgg cctcggctgc
     1321 tcgatccagc tgctggatcg cttcttttac ggccgcggcg gcctggtctt gttttccggc
     1381 cgcgattgcc gttcgggcaa ttctgacctg agtccgggcc cgggtgcgga caggcctatt
     1441 ccgctcccgg cgtttctcat tctgccggat ccgtttcttc gcagagcgcg tgttggccaa
     1501 ttcaatccct ttgctacggc aactgataat tgtagccgat gcaactttgc ttgtccagag
     1561 accggcctgg accggcgacg gtcggctgct ctcaaagctc caccacctgc accgctccga
     1621 ccgcgtcgag gaagcacagc atgccgcgcg gcttctcgcc cagaatgctc gcggccgaac
     1681 ggacgtagcg ctcgacctgc ttgcggtgac gctcaactgc agcgggcagg tccgcctcgc
     1741 tgcgcagcgc atccgtctta aaatccacga tcttccagcc ctgctcgccg cgccacagta
     1801 ggtcgatctg gccgctctgg acctgatcgg ctgggtcgga ggtcgtaaac gggatctcgc
     1861 gctgggccgc ctccgccggg ccaatctcct cccacaaggc atgcgccgcc agccgcccga
     1921 gcatctcggt cgattccgcg accgcagccg cgcgctgacg ctggtccacc aatccggctc
     1981 cgaaggcttc gttttccatg aacggctcgt atccggcgct gcccggcatc gcagaacgct
     2041 ggatggcggc gtgaaccata cgaccaacgg cggccgccgg ggcgc
//
LOCUS       DATDPQ010000249         3828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_901363, whole genome shotgun sequence.
ACCESSION   DATDPQ010000249 DATDPQ010000000
VERSION     DATDPQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901363"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VI520_05575"
     CDS             <1..1233
                     /locus_tag="VI520_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37386.1"
                     /translation="LLLAVLYLAFLIAVAGGVPRFVQLLSAHFLLSLAALFLALSMVA
                     QFVLPVRDSRGRRAVVSRLLNYTLGERGSVTFVKEGRAQAESGGRGPGVIWVDYLSAA
                     VLRTDRDFTRTILPGQLAFTVPGERLAEGLDLRRQRRSLQSSPPPAGSPATAQEVSAM
                     AVTRDGIPISASLRVSFVLERRPPFKRGTIADPPPISPSAPALQAAASGRAVAGEDRL
                     PWSDLPQRLVVELWREFVKEHPLDDFLTHPAATVAAIATQVQERLVAGSGRSVPGDET
                     RLLRERGIQILEVAIEDPQLPEEIREERLNAWFDRWAGPVQKQLADAETQLRAAGRRG
                     EAETSARLLDRLTHKLRQQLRLEPAPGPRDTLILLLEDAAEYCPEDNRLAHLASPVRS
                     VLEEVKARDPEGLPRGEG"
     gene            1266..2666
                     /locus_tag="VI520_05580"
     CDS             1266..2666
                     /locus_tag="VI520_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37387.1"
                     /translation="MDRGIGSLPIGMLLDRGRAGARARLVLTLSLVVGYWSLVTLVLD
                     FPPLIAAETLERLPVFIAILLDVVTSFFSPQVIVFLFPVLIAGVLALHLGAAYLTDLF
                     ELESTGIAYRYLLGAVFGLSYPVLTIDRGQVETLDQANPLLRIGGPGYLNLHLGFAAV
                     FETIDGQPKIYGPTRERSEFFIQGFERLREVIDLRDQLRQLDEVRTVTEDGIVVYARD
                     VQIVFRAYGGEQRTAGSPLPVETRSVLSLAYGGQVAEAGPSHWTDTLPDLARNELEAF
                     VAGRTLEEFLALPQQASKRRESGPTHVHIPRRELTESFHTAARRARLRELGLELVWLG
                     VGTWEVRDDQILSTDLEIAAGQTLTGVARDELRARRLGSPEHLTRERQLRAFALSQEV
                     IEELIAAWGEVKLPERFRSHELLDRFRRRLIELKVRVEDQHRVEPSSTHDLDLPERLA
                     EVLHHLETLTERSPTR"
     gene            2690..3601
                     /locus_tag="VI520_05585"
     CDS             2690..3601
                     /locus_tag="VI520_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HLF37388.1"
                     /translation="MKPSPPAWLHVHPETREALEGRRPVVALESAVITHGLPRPINLE
                     LARRMEAEVRKVGGVPATTAVLSGMIRLGMSSEELESLALDLSAVKVGRRDLGPVRTG
                     GQSGGTTVSAAIFIAATAGIRILATGGIGGVHRGESGDVSADLPELGRTPVGVVCSGA
                     KTILDLPRTLEWLETGGILVVGYQTHEFPAFCAQGSGLTVGTSVETPAEAARLLRSHW
                     ELGSASAALICVPCPESDSLPREDVEQAIEQAEREAEDKGVRGPALSPFLLTRLAELT
                     NGRALKANLALLRNNARVAAEIALALG"
BASE COUNT          633 a         1260 c         1234 g          701 t
ORIGIN      
        1 ctgctgcttg cggtcctgta ccttgcattc ctgatcgcgg tcgccggcgg cgttccacgc
       61 ttcgtccagc tgctgagtgc ccacttcctg ctctcgctcg ccgcgctgtt tcttgccctt
      121 tctatggtgg ctcagttcgt gctgccggtt cgggactcgc ggggacgccg ggcggtggtc
      181 agccggctgc tgaactacac cctcggcgag cggggcagcg tgaccttcgt aaaggagggg
      241 cgggcccaag ctgagtcggg gggccgggga cccggggtga tctgggtgga ctatttgagc
      301 gcggcggtgc tccgcaccga tcgagatttc acccgcacca tcctgcccgg ccaactggct
      361 ttcacggtcc cgggcgagcg cctggcggag gggctcgatc tgcggcgcca gcgacgcagc
      421 ctgcaaagtt ctccgccgcc ggcgggctct ccggccaccg cgcaggaagt cagtgcgatg
      481 gccgtgactc gcgacggaat tccgatttcg gcctcgctgc gagtgagctt cgtcttggaa
      541 cgccggcctc cgttcaaacg cggcacgatc gccgaccctc cgccgatctc tccctcggcc
      601 cccgccctgc aggccgcggc ctccggaagg gcggttgccg gcgaggatcg actgccgtgg
      661 agcgatctcc cgcagcggct ggtcgtcgag ctctggcgag aattcgtcaa agagcatccg
      721 ctggacgact ttctcaccca ccccgcggcg accgtagccg cgattgctac ccaagtccag
      781 gaacgattgg tggcgggaag cggccgctcc gtgccgggcg acgaaactcg attgctgcgc
      841 gagcgcggca ttcagattct agaggtggcc atcgaagatc cgcagctgcc ggaagagatc
      901 cgggaagagc ggctgaatgc ttggttcgat cgctgggcgg gtccggtcca gaaacagctt
      961 gcggatgccg agacccaatt gcgagcggcc ggccgacggg gcgaagccga gaccagcgcc
     1021 cgcctgctag accggctcac ccacaagctt cgccagcagc tgcgcctcga accggcgccc
     1081 gggccgcgtg acaccctgat cctgctgctg gaagatgcgg ccgaatattg cccggaagac
     1141 aaccggttgg cccatctggc gagcccggtt cgttcagttc tcgaagaggt caaggcccgc
     1201 gatccggaag gtttgccgcg gggagagggc tgatggcgcg aatgtcttca ccccacagca
     1261 ctccgatgga ccgaggaatc gggagcctcc cgattgggat gctgttggac cgaggtcggg
     1321 ccggagcccg tgcccgcctg gtactcacgc tttcgctggt ggtcggctat tggtcgctcg
     1381 tgactctggt gctggacttt cctcctttga tagcagccga gaccctcgag cgactgccgg
     1441 tcttcattgc catcttgctt gacgtcgtga cctctttctt cagcccgcag gtgatcgtct
     1501 tcttgttccc ggtcttgatc gccggagtgc tcgcactgca cctgggagca gcctatctca
     1561 ccgatctgtt cgagctcgag tccactggta tcgcctaccg ctatttgctc ggggcggtct
     1621 tcggtctgtc gtatcccgtg cttacgattg accgcggcca ggtcgagacg cttgatcaag
     1681 ccaatccact gctccgcatc ggaggaccgg gttatctcaa tctgcacctc gggttcgcgg
     1741 cagtcttcga gacgatcgac ggccaaccca aaatctatgg cccgacccgc gagcggagcg
     1801 aattcttcat ccagggtttt gaacggctgc gggaagtcat cgacctgcgc gatcagctac
     1861 gccagctcga tgaagtacgg acggtcaccg aggatggcat tgtcgtctac gcccgtgacg
     1921 ttcagatcgt ctttcgcgcc tatggtgggg agcagcggac cgccggcagc ccgcttccag
     1981 tcgagacccg cagcgtgctc agcctcgcct atgggggtca ggtggctgag gccgggccga
     2041 gccattggac cgataccctc cccgacttgg cccgcaacga gctggaagcg ttcgtcgccg
     2101 gccgcacgct ggaagagttt ctggccttgc cgcagcaagc ttccaagcgg cgtgagtcgg
     2161 ggccgaccca tgtccacatc ccacgccgcg agctcactga gagtttccac accgccgccc
     2221 ggcgcgcaag attgagagag ctcggcttgg aattggtctg gcttggggtc gggacctggg
     2281 aagttcggga cgaccagatc ctatccaccg accttgaaat cgcagccggg cagaccctca
     2341 ccggagtcgc ccgggacgaa ctgcgggccc gacggctggg ctcccccgaa cacctcaccc
     2401 gggaacggca attacgggct ttcgccctca gccaggaggt catcgaggag ctgattgcgg
     2461 catggggcga ggtgaaattg cctgagcgct tccgcagcca cgaactgctc gaccgcttca
     2521 ggcgccgact gatcgagctc aaagtgcgag tcgaagatca gcaccgggtc gagccgtcgt
     2581 caacacatga cctggacctt cccgagcgat tggcggaggt cctgcaccac ctcgaaactc
     2641 tgaccgaacg cagccccacc cgctgaggcc ggggccggtt tccgctgccg tgaagccaag
     2701 tccgccagcc tggttgcacg tccaccccga gacccgcgag gcactggagg gtcggcggcc
     2761 ggtcgttgcg cttgaatcgg ccgtgatcac tcacggtctg ccccgcccca tcaacctcga
     2821 gctagccagg cgaatggaag cagaggtccg taaggtaggc ggggtgccgg caaccaccgc
     2881 agtactcagc ggaatgatcc gcctggggat gagttccgag gagctggaat cgttggccct
     2941 cgatctcagc gcggtgaaag tcggccggcg ggatctggga cccgtgcgga ccggcggcca
     3001 gtcgggcggg accaccgttt cagcggccat cttcatcgcc gccaccgcag gcatccggat
     3061 tttggccacc ggcggaatcg gcggggttca tcgcggcgag tccggtgatg tctctgccga
     3121 tctccccgag ctgggacgca cgccggtggg ggtcgtgtgt tcgggcgcca agacgatcct
     3181 ggacttgccc cgcaccctcg aatggttgga gaccggaggg attctggtcg tgggttatca
     3241 aacccacgaa ttccccgctt tctgcgccca gggatccggc ctgacggtcg gtaccagcgt
     3301 ggagacgccg gccgaggcgg cccggttgct tcgctcccac tgggagctcg gctcggcttc
     3361 cgcggccctg atctgcgtgc cgtgcccaga gtccgactcc ctgccaaggg aagatgtcga
     3421 gcaagccatt gagcaggccg aacgggaggc cgaggacaag ggagtgcgcg gtccggccct
     3481 ctcacccttc ctgctcactc gcctggcgga actgaccaac ggccgagctc tcaaggccaa
     3541 cctggccctg ctgcgcaata acgcgcgggt cgcggcggaa atcgctttgg cactcggatg
     3601 atctcctgcg ccatcccgag gggccattaa catcgccgtc gcatgtggta tgttagaatc
     3661 gctcggctaa tgcccggcag tacatgaaca tgatcgaagt cggcatcgac agcatccgcg
     3721 tcagtctcat gtcccaacac cgcatcgtca ttctcaaaga cgtcgattcg gaccgctttc
     3781 tacccatctg gatcgggccc tatgaggcgg aggccatcac cgccagtc
//
LOCUS       DATDPQ010000250         3913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3282725, whole genome shotgun sequence.
ACCESSION   DATDPQ010000250 DATDPQ010000000
VERSION     DATDPQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282725"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VI520_05590"
     CDS             complement(<1..1286)
                     /locus_tag="VI520_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HLF37389.1"
                     /translation="MIRNPVTLLTEEQVERIHEASLEILANIGLLVRNEKARRIFEQH
                     GCRVDSETQVVRLPGAVVEEFRASIPPSFTFAGRDPQFDRVIPADGPLIVTGSSAPNI
                     IDPLTGHERRARSDDIARIAHLVNELPGFDIFSISTLADDAPPGQFSVSRFYPALKYC
                     AKPLRGSGPPEDTPQILQLAYLIAGSEAAYRAHPFITHHYCPVVSPLTMDFDSTEVMI
                     YFSEQGLPCYPTIVPNAGLTSPLTLTGTLAQGNAEFLAAAVLEQMVRPGKPTIYSSLP
                     TVADMRTGAYAPGGIETGILFIGIAQLARFYNVPCGGYIGLTNAKLNDAQSGFETGMS
                     TLGGVLAGVDMLNMAGLLDALMTFDFGKAYIDHEIALMLKKAKRGLEFSEQELALDVL
                     AEVGPAGMFADHLHSLERMRTTGLLPEVADRDPRAQ"
     gene            complement(1301..2026)
                     /locus_tag="VI520_05595"
     CDS             complement(1301..2026)
                     /locus_tag="VI520_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLF37390.1"
                     /translation="MVAALPRYVQIAESLLEQIESGQLKPGDRLPAERAMSEMLSVNR
                     MTVRRALRVLESRGLVVRLQGDGTYVAEPKIERQAARLTSFTVGMQRRGLQPGARIVS
                     LEQRPVEASVGALLQLPISAPVYSLLRLRSINHEPVMLERYTIPIQRFPGLERHDLEQ
                     RSVYEVFQTEYQVKVERARQSLEPVVATEFEAELLAIPSGSPLMLERRLSFDSDGRPV
                     EHGTDLYRGDRFRFVTELAPLEM"
     gene            2182..2871
                     /gene="nth"
                     /locus_tag="VI520_05600"
     CDS             2182..2871
                     /gene="nth"
                     /locus_tag="VI520_05600"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLF37391.1"
                     /translation="MRSPRQLAREVHRRLLAVYGEPTWRDRKPPVDELVSTILSQNTN
                     DLNRDRAFETLRKRFPTWEAVRDAKPRQVVSAIRVAGISNQKGPRIQAALRAITDERG
                     RLELDFLRKQPPEAVRAWLLQIEGVGPKTAAIVMQFALDMPAFPVDTHIHRVSGRIGL
                     RPPKAGREKSHEILAGLFAPETYYAAHLNLIRHGREICKAGRPRCEQCSLTDLCDYYA
                     GLDPKPGAAAG"
     gene            2926..3609
                     /locus_tag="VI520_05605"
     CDS             2926..3609
                     /locus_tag="VI520_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLF37392.1"
                     /translation="MSVSITWYGHAALGLDIDGVKVTVDPFLNGNPAATIKPEQVQAD
                     YILITHGHGDHVGDAIAIAKRTGATVVSVNEIAVWFQKQGLKSHGQHLGGGHQHEFGY
                     LKLTLALHGSGLPDGSYGGNPCGLLITTVSGEKVYLAGDTGLFGDMRLIGEEGLDLAV
                     VPIGDNYTMGPDDALRAVKLLQPKHVIPIHYDTWDVIAQDPQAWAERVTAETSAQPHV
                     LKPGQTFSL"
     gene            3669..>3913
                     /locus_tag="VI520_05610"
     CDS             3669..>3913
                     /locus_tag="VI520_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF37393.1"
                     /translation="MAGLTLLALGFGMATNTLDPVLLSYKAALLAPDHRNTALGLTTA
                     AGLLVATFTQPVVGALSDRTRSRWGRRIPYFIVGTLLT"
BASE COUNT          716 a         1233 c         1270 g          694 t
ORIGIN      
        1 cactgcgccc gcggatcccg gtcggcgact tcgggcaaca gcccggtggt ccgcattcgt
       61 tccaggctgt gcaggtgatc ggcgaacatc cctgccgggc cgacctcggc caacacgtct
      121 agggccagtt cctgctcgct gaactccaga ccccgcttgg ccttcttcag catcagtgca
      181 atctcgtgat cgatgtaggc cttgccgaaa tcaaaggtca tcagggcgtc cagcagcccg
      241 gccatattca gcatgtccac cccggccagc acgcctccca gggtggacat cccggtctcg
      301 aatcccgact gggcatcgtt gagcttggcg ttggtcaggc caatgtaccc gccgcacgga
      361 acgttgtaga agcgggccag ctgcgcgatt ccgataaaca gaattccagt ctcaatgccg
      421 ccgggcgcat aggcgccggt gcgcatgtcg gcaacggtcg gcaagctgct gtagatggtt
      481 ggcttccccg gtcggaccat ttgctcgagc accgccgcgg ccaggaattc ggcattgccc
      541 tgggctaggg tcccggtcag ggtgagcggg gaggtcagcc cggcgttagg tacgatggtg
      601 gggtagcagg gcaggccctg ctcgctgaaa tagatcatca cctcggtcga gtcgaaatcc
      661 atggtgagcg gcgagacgac cggacagtaa tggtgggtga tgaagggatg cgcccggtag
      721 gcggcttcgc ttcccgcgat caggtaagcc agctgcagga tctggggagt atcttccggg
      781 gggccgctcc cgcggagtgg cttggcgcag tacttgaggg ccggatagaa gcgagacacg
      841 ctgaattgtc cgggcggggc atcgtcggcc agagtggaaa tggaaaatat atcgaagcca
      901 ggcagctcgt tgaccaggtg ggcgatgcga gcgatgtcat ccgagcgggc ccggcgctcg
      961 tggccggtca gcggatcgat gatgttgggg gccgagctgc cggtgacgat cagcggaccg
     1021 tcggcgggaa tgacccggtc gaactgaggg tcgcggccgg cgaaagtgaa gctgggcggg
     1081 atggaggccc ggaactcctc gacgaccgcg ccgggcagtc gaaccacctg ggtttcggag
     1141 tcgacccggc agccgtgctg ctcaaagatg cggcgggcct tctcgttgcg gaccagtagc
     1201 ccgatgttgg ccaggatctc gagcgaggcc tcatggatgc gctcgacttg ctcttcggtt
     1261 aacagggtca cgggattccg aatcatggcg aacctccctt tcacatctcc agcggagcga
     1321 gctcggtgac gaagcggaat cggtcacccc ggtacaagtc ggtcccgtgt tcaaccggtc
     1381 ggccatcgga gtcgaatgag agccgccgct cgagcatcaa gggcgaaccg ctcgggatgg
     1441 ccagcaactc ggcctcgaac tcagtggcga cgaccggctc cagactctgg cgagcgcgct
     1501 ctaccttgac ctgatactcg gtctgaaaca cctcgtagac cgagcgctgc tccagatcgt
     1561 gccgctccag tccgggaaag cgctggatgg ggatcgtgta gcgctcgagc atgacgggct
     1621 cgtggttaat cgagcggagt cggagcaggg agtagaccgg agcggagatc ggcagctgca
     1681 gcagcgcccc gaccgaagcc tcgaccggtc gctgttccaa gctcacgatt cgggctccgg
     1741 gctgcaaacc gcggcgctgc atcccgaccg tgaaggaggt caggcgggca gcctggcgct
     1801 cgatcttcgg ctcggccacg taggtcccgt ctccttgcag gcgcacgacc agcccgcggg
     1861 actcgagcac ccgcagggca cgccgcacgg tcatccgatt gacgctcagc atctcgctca
     1921 tggcgcgttc ggccgggagc cggtcgcccg gtttcaattg gccggactcg atctgttcga
     1981 gcagcgactc ggcgatctgg acgtaccgcg ggagcgcggc gaccaatcgg ttgaccgggg
     2041 gatcgaaaat ggctatgtga aatccttcac aaatgaggtt ctgtctggat tactggtgta
     2101 taccattcac ttcagtatag tggtttaggc gatggcctgt caattggcac gctttgccca
     2161 tgcccttggt agaatccccc cgtgcgttcg ccccgccagt tagcccggga ggtgcaccga
     2221 cggctgttag cggtctacgg cgagcccacc tggcgagacc ggaagccgcc ggttgacgag
     2281 ctggtctcga ccatcctctc ccagaacacc aacgacctga accgcgatcg agccttcgaa
     2341 accctgcgca agcgcttccc aacctgggag gcggtccgcg acgccaagcc ccgccaggtc
     2401 gtcagcgcca tccgggtggc agggatttcc aatcagaaag ggcctcgcat tcaggccgcg
     2461 ctgcgtgcga tcacggacga gcgagggcgc ctcgagctgg acttcttaag gaagcagccc
     2521 ccggaggccg tccgagcttg gttgctgcaa atagagggtg tcgggccgaa gaccgccgcg
     2581 atcgtgatgc agttcgccct cgatatgccc gcctttccgg tggataccca tattcaccgg
     2641 gtaagcggcc ggatcggcct ccgccctccg aaggccggcc gcgagaagtc ccacgagatt
     2701 ctcgccgggc tcttcgctcc ggagacgtat tacgccgccc acctgaatct cattcggcat
     2761 ggacgcgaga tctgcaaggc cggtcgcccg cgctgcgagc agtgcagtct caccgaccta
     2821 tgtgattact atgcaggctt ggatcccaaa ccgggtgcgg ccgccgggtg aggcctgtgc
     2881 ccatctggtc tatactcaaa ccgcagaatc tctccggagg acagcatgtc cgtcagcatc
     2941 acctggtacg gccacgccgc gttaggtctg gacatcgacg gggtcaaggt cacggtcgat
     3001 ccatttctaa acgggaatcc ggccgcgacg atcaaacccg agcaggtgca ggccgattac
     3061 atattgatta cccatggcca cggagaccac gttggggacg caatcgcgat cgccaaacgg
     3121 actggagcca cggtggtgag cgtgaacgag atcgcagtct ggttccagaa gcagggcctc
     3181 aagtctcacg gccagcattt gggcggaggc catcagcatg aattcggtta tctcaagctc
     3241 accctggcgc tgcacggttc cggcctgccg gatggttcct acggcggtaa cccctgtggg
     3301 ctgctgatca cgacggtgag cggcgagaag gtttacctgg cgggcgacac cggcctgttc
     3361 ggtgacatgc gactgatcgg ggaggaaggg ttggacctgg cggtcgtgcc gatcggcgac
     3421 aactacacca tgggtcctga cgacgcgctg cgggcagtga agctgttgca gccaaagcac
     3481 gtcatcccca ttcactacga cacctgggac gtaatcgccc aggatccgca ggcctgggcc
     3541 gagcgggtga cggcggagac ctcggcccaa ccccacgtcc tcaaaccagg ccagaccttc
     3601 agcctgtagc caccctgagc gaactcgcga ctgctgctga tcgccccgcc gacccgggct
     3661 ggctccgcat ggccggcctg accctgcttg cgctcggctt cggcatggcg accaacaccc
     3721 tggatccggt gttgctcagc tacaaggcgg cgctgcttgc accggatcat cgcaacactg
     3781 ccctcggcct aaccaccgcc gccgggctcc tggtggccac tttcacccag ccggtggttg
     3841 gagccctctc cgatcggacc cgctcgcgct ggggaaggcg gattccttac ttcattgtgg
     3901 gaaccctgct gac
//
LOCUS       DATDPQ010000251         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3283449, whole genome shotgun sequence.
ACCESSION   DATDPQ010000251 DATDPQ010000000
VERSION     DATDPQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283449"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VI520_05615"
     CDS             complement(<1..518)
                     /locus_tag="VI520_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLF37394.1"
                     /translation="MKLQENQLPPAAKELLQKFASRRATIGVVGLGYVGLPLAVLLAE
                     AGYRVLGVDRDSQKVSQINEGNSYVMDVPASAVAAVVQGGKLSATSSYADLAAADGVS
                     ICVPTPLRKTGDPDLSYILDAASALQPALHSPMAIILESTTYPGTTRELLLPELENSG
                     LKVGEDLFLAFSP"
     gene            complement(519..1136)
                     /locus_tag="VI520_05620"
     CDS             complement(519..1136)
                     /locus_tag="VI520_05620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLF37395.1"
                     /translation="MNPEQELKSVADQVMLCTDCKLHASRKLAVPGEGPAKAELMFIG
                     EGPGFHENEQGRPFVGAAGSFLEELLASIGMKRDQVYITNIVKCRPPGNRDPQPQEIE
                     ACSGYLERQIRSINPKVIVTLGRFSMARFIPNARISAIHGRAQAIGGRTVVAMYHPAA
                     ALHQPSLRRTIEQDFARLPALIAKAGHPAPTEQAPPASIEQLSLF"
     gene            complement(1133..1624)
                     /gene="rimI"
                     /locus_tag="VI520_05625"
     CDS             complement(1133..1624)
                     /gene="rimI"
                     /locus_tag="VI520_05625"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HLF37396.1"
                     /translation="MIAIPAQVHIREMTLQDLPEVLEIDRASFPVPWPERSYRFELVE
                     NPAARLFVAEKSDEPESQVVGFIGYWLITDEVHISTFAIHPDYRMQGIGELMLVRALR
                     TARARGASMATLEVRISNLPAIHLYEKLGFEQVGKRAGYYRDNGEDALLMTLHRLSEG
                     GEP"
     gene            complement(1621..2277)
                     /gene="tsaB"
                     /locus_tag="VI520_05630"
     CDS             complement(1621..2277)
                     /gene="tsaB"
                     /locus_tag="VI520_05630"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLF37397.1"
                     /translation="MLLAIDTASRMLGVALHDGGRVLSESTWYGSGHHTTELAPEVAL
                     ALSRAAVEVSELTAVAVALGPGSYTGLRIGLALAKGLSLSHNLQVVGIPTLDILANPQ
                     PPREGPMLAVMQAGRGRVAGVWYKWSRKGWSARKGALNLRWEEILAELKQATYICGEL
                     DLESRETLHKSEFVELAPPALCVRRPSVLAELALEKVGHGKPEDTARLAPIYLDNSTA
                     "
     gene            complement(2280..2777)
                     /gene="tsaE"
                     /locus_tag="VI520_05635"
     CDS             complement(2280..2777)
                     /gene="tsaE"
                     /locus_tag="VI520_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLF37398.1"
                     /translation="MPILDERSLEFLSHSPEQTERLGVRLGELARPGDVFCLAGDLGA
                     GKTTLAKGIARGWGALDQVTSPSFVLINEYRRADSHRLIHLDAFRLESRAEAAALGLS
                     DLFDGVGPVLVEWPERVQELLPAERLWISLHWVDEMRRNFRFEAHGLHYERLLRRFRQ
                     AAFGG"
     gene            2861..>3577
                     /locus_tag="VI520_05640"
     CDS             2861..>3577
                     /locus_tag="VI520_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLF37399.1"
                     /translation="MRVLVISDVHANLTALNAVLTDAAQRLPAYEAVWCLGDVVGYGP
                     DPNECVEVVRSLPGLVCLIGNHDQAALGITPLTRFNHDARAAAAWTQEVLSEESAEFL
                     RTLPSDAQVDGFTLAHGSPRQPVWEYILDPRTADANFNEFQTDYCLVGHSHLPLVFQR
                     GTADSFALSRQVAGEQVVSLSPRMILNPGSVGQPRDMDPRAAYALLDMKTRSWEARRA
                     AYDIGDVQLRMLKAGLPERQA"
BASE COUNT          652 a         1183 c         1074 g          668 t
ORIGIN      
        1 ggagaaaagg ccaggaacaa atcctccccg acttttagcc ccgagttctc cagctccggc
       61 agcaacaact ctcgggtcgt acccggatag gtggtcgatt caaggatgat cgccatcggc
      121 gagtgaagcg ccggctgaag cgcgctggcc gcgtcgagga tataggagag gtcgggatcg
      181 ccagtcttcc gtaacggagt cgggacgcag atgctcactc catcggccgc agccagatcg
      241 gcgtaggagc tggtggcaga aagcttgccc ccctgcacca ctgccgcgac cgcgcttgcc
      301 ggcacatcca tcacgtagct gttcccttca ttgatctggc tgaccttctg cgaatcgcgg
      361 tccaccccca aaacccggta cccggcctcc gccaggagca ccgccagcgg caggccgaca
      421 tagcccaggc cgactactcc tatcgtggcg cgtcggctgg cgaacttttg cagcaactcc
      481 ttcgctgcag gcgggagctg attctcttga agcttcaact aaaacaggct caattgctcg
      541 atgcttgccg gcggagcttg ctcggtgggc gcggggtggc cggccttggc gatcagggcc
      601 ggcaggcggg cgaagtcttg ctcgatcgtc cgacgcagcg acggctggtg gagagcggcc
      661 gccggatgat acatggcgac caccgtccgg ccgcctatcg cctgcgcccg gccatggatg
      721 gcggagatgc gagcgttggg gatgaagcgc gccatcgaga atcgcccaag agtgacaatg
      781 actttcggat tgatgctccg aatttgccgt tccaggtaac cggaacaggc ttcgatctct
      841 tggggttgag gatcgcggtt gcccggcggc cggcacttga cgatgttcgt gatatagacc
      901 tgatcccgct tcattccgat cgaggccagc agctcctcca ggaagctgcc cgccgcgccg
      961 acaaacggcc ggccctgctc gttctcgtga aaaccaggcc cctctccgat aaacatcagt
     1021 tcggccttcg ccggtccttc gccggggacc gcaagtttgc gcgaggcgtg cagcttgcaa
     1081 tcggtgcaca gcatgacctg gtcggcgacc gacttgagct cctgctccgg attcacggtt
     1141 cccctccttc actgagtctg tgcagcgtca tcaggagggc gtcttccccg ttgtctcgat
     1201 agtagcccgc ccgcttgccc acctgctcga agcccagctt ctcgtagaga tgaatcgccg
     1261 gcaaattgga aatcctgacc tccagagtcg ccatacttgc acctcgggct cgagcggtcc
     1321 gcagggcgcg caccaacatc agctcgccga tcccctgcat ccggtagtcc ggatgaatcg
     1381 cgaaggtact gatgtgaact tcgtcggtga tcagccaata accaatgaac ccgaccacct
     1441 ggctttctgg ctcatccgac ttttctgcaa caaagagccg ggcggccggg ttttcgacga
     1501 gctcaaaccg gtaactgcgc tccggccagg ggaccggaaa agaggctcgg tcgatctcga
     1561 gaacctccgg caggtcctgc agggtcatct cccggatgtg gacctgcgca ggaattgcga
     1621 tcacgctgtg ctgttgtcga gataaatcgg agcgagcctg gccgtatcct cgggcttccc
     1681 gtgcccaact ttctccaggg ccaattcagc gaggacgctt gggcgacgga cacacagcgc
     1741 aggcggggct agctcgacaa actccgattt gtgaagagtc tcgcgactct ccagatcgag
     1801 ctctccgcaa atgtaggtgg cctgcttcag ctcagccagg atctcttccc accggaggtt
     1861 gagcgcgcct tttcgagcgg accaaccctt gcggctccat ttgtaccaca cccccgccac
     1921 tcggccgcga cccgcctgca tcaccgcaag catcggtccc tcccgcgggg gctggggatt
     1981 ggccaagatg tcgagagtcg ggatgccgac tacctgcaga ttgtgggaga ggctgagacc
     2041 cttggctagc gccaggccga tccgcagtcc ggtgtacgat ccgggaccca gcgcgactgc
     2101 gaccgcagtc aactccgaga cttctacagc cgcccggctc agtgccaacg ccacctcggg
     2161 tgccagttcg gtggtgtgat gtccggaccc gtaccaggtg ctttcgctga gtacccgccc
     2221 gccgtcatgc agcgcaactc cgagcatgcg ggaagccgta tcaatcgcca acagcatcgt
     2281 cagcctccga aggctgcctg gcggaatcgc cgaagcagtc tctcgtagtg cagcccgtgg
     2341 gcttcgaagc ggaagttgcg gcgcatttca tcgacccagt gcaggctgat ccagagccgc
     2401 tcggccggga gcagctcctg tactcgctct ggccactcca ccaacaccgg cccgacccca
     2461 tcgaacaaat ccgagaggcc cagggccgcg gcttcggctc ggctctcgag ccgaaatgca
     2521 tccaaatgga tgaggcggtg cgagtcggcc cggcggtatt cgttgatcag gacgaaactg
     2581 ggactggtga cctgatctag cgccccccag ccgcgggcaa tccccttagc cagggtcgtc
     2641 ttgcctgccc ccaggtcgcc ggccaggcag aacacatccc ccggtctggc cagttccccc
     2701 aagcgaactc ccaggcgttc cgtttgctct ggactatggc tcaggaactc caggctccgc
     2761 tcatcgagga tgggcatcga aaggggccgg attatacgtc cgaggtaccc ccctcacaag
     2821 cactagcctt cgagtatgct tgcatactag aatctttcga atgcgcgtcc tggttatctc
     2881 tgatgtacat gccaacctca cggcgctcaa tgcggtactc accgacgcag cgcagcgcct
     2941 gcctgcctac gaggcggttt ggtgcctggg cgacgttgtg ggatacgggc cagatccgaa
     3001 cgagtgtgtg gaggtcgtgc gctcgctccc cgggctggtc tgcctgatcg gcaatcatga
     3061 tcaggccgca ttgggaatca cgccgcttac ccgcttcaac cacgatgctc gggccgcggc
     3121 ggcctggact caagaagtgc tctcggaaga gagcgccgag tttcttcgca cccttcccag
     3181 cgacgcccag gtcgacggct tcaccctggc ccacggcagt ccgcgccagc cggtttggga
     3241 gtacatcctt gacccacgga ccgcagacgc gaatttcaac gaattccaga ccgattactg
     3301 cctggtcggc cactcgcact tgccgctggt cttccagcgc gggacggccg actcattcgc
     3361 cctctcccgt caggtagccg gcgagcaggt ggtctcgctc tcgccgcgca tgatcctgaa
     3421 tcccggttcg gttggccagc cgcgcgacat ggacccccgg gccgcctacg cgttgctcga
     3481 tatgaagact cgcagttggg aagcccgacg ggcggcctac gacattggtg acgtccagct
     3541 ccggatgttg aaggccggcc tgcccgaacg gcaggcg
//
LOCUS       DATDPQ010000252         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3511826, whole genome shotgun sequence.
ACCESSION   DATDPQ010000252 DATDPQ010000000
VERSION     DATDPQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511826"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VI520_05645"
     CDS             complement(<1..146)
                     /locus_tag="VI520_05645"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLF37400.1"
                     /translation="MTELIHVSVAWPYANGDMHVGHLAGAYLPADIFARYHRLKGNRV
                     LMVSG"
     gene            complement(497..2086)
                     /locus_tag="VI520_05650"
     CDS             complement(497..2086)
                     /locus_tag="VI520_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37401.1"
                     /translation="MRAIFLTTLVILLTACSSSAVIGEEPAPPAPAAGNLASSSSARR
                     EAELPVPYQETGGDRPGSFSESSASENSPAEGPALFPLEASVPLVAWNSEQRRYELRL
                     VDPMTGYVFPDRPPIDVSHSTRNAPNLALSPDGSRLAATTGVGSFCEAYAGYPSCQPS
                     ADSIRLIDLQTWREAGADLLAHIDASPSPFKGWASPLVFSQDSKRFAVALNDRAGTSV
                     LIYDSGTGQFIGKQALAIRPRLMEFTSDGSQLMIYGSPLAEIPGISQPEPPSALLLDA
                     STLETQWKTLLPGILDGTWCLESCETSSESARFVYWSPAVVFDSERGRLYIVHADEDR
                     LTTVNFLGRNVRNLAIGPAPNWIERLLALTATVAEAKYWPQGGIKSAVLSQDGSIIYV
                     VGRTMTLSKDSNGEWRGSETPLGLTVIEAVNGHELATRESDAGRIRSSTDHRYIILDW
                     GFLGTVEVLEAETLEQVGAIEDWEVRLGQRLDGRAVILLAGWTGESQTAPTRFAMLEL
                     EPFDVITPWSVSGEAFWATAP"
     gene            complement(2182..>2827)
                     /locus_tag="VI520_05655"
     CDS             complement(2182..>2827)
                     /locus_tag="VI520_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37402.1"
                     /translation="AGRGEAIRQRYLRDGEQQAKYFAADLEDTAIDQAIGLQEQGILP
                     ASLQFIRSADIGEPSGVIQYLARQELAGEPTVMMVGNGFHEIRNQTNEKMVEVFRGYE
                     HAGFVLIFTEESALDDEALLDTAWNTYHAGFRYVHEMSGQGLRPPAEGEHGAQRWSWR
                     KCGELAGYVFLDEFSYRSRSIYPYRRPTHKNPSISVTYFCIPAKLAQELGIKPD"
BASE COUNT          537 a          894 c          852 g          544 t
ORIGIN      
        1 ccagacacca tcaggacccg gttgcccttc agccggtgat agcgggcgaa gatgtcggcc
       61 ggtaggtagg cccctgccag gtggccgacg tgcatgtccc cgttggcata gggccaggcg
      121 acggaaacgt ggattagctc agtcatcgtt tgtcacccat ttccttcgat tcgctctgga
      181 ggggggagca ttccccgagc cggaaaacaa aacgccagcc cgaggctggc ggtgggtgcg
      241 ggccggcggc gccttcagcc ggcgcgtcgc tcctcagccg ataggcctcg tcccatcggc
      301 ggccggccgc tgcgcatgtc catagagcaa tccaacccgc ataatccaag catattcgag
      361 accagccaaa gaggattcta actgcgtcga agcggaagtc aagtggatga cgtgcaccgg
      421 tccatcggat gcaacggctt ggccggtgtc tcgcggggaa cttgaatcag atggcccggc
      481 ggggtagggc cctgcctcac ggagcggtcg cccagaacgc ctcgccgctc accgaccacg
      541 gtgtgatcac gtcgaaaggc tcgagttcaa gcatcgcgaa acgggtcgga gcggtctgcg
      601 attctccagt ccaacctgcc agcaaaataa ccgcccggcc atccaatcgc tggcctaggc
      661 gaacttccca gtcttcaata gcgccgacct gctcaagagt ctccgcctcc aagacttcga
      721 cggtacccag gaacccccag tccaggatta tgtagcggtg gtcggtcgag ctgcggatcc
      781 ggccggcgtc gctctctcgg gtcgccagct cgtgaccgtt gacggcttca atcacggtca
      841 agcccagtgg agtctcgctc ccacgccact ccccattgga gtccttggac agcgtcattg
      901 ttcggccgac cacgtagata atcgagccgt cctgcgagag aacggcgctt ttgatcccgc
      961 cctgcggcca atacttggct tccgctacgg tcgcggtgag ggccagcagc ctctcgatcc
     1021 agttgggggc cgggccgatc gcgagattcc gcacattgcg ccccaggaaa ttgacggttg
     1081 taagccggtc ctcgtccgca tggacgatgt agagccggcc ccgctccgag tcgaacacga
     1141 ccgcaggaga ccagtacacg aatcgagcgg actcgctgga ggtttcgcag ctctccaggc
     1201 accaggttcc atccaaaata ccaggaagga gcgttttcca ttgagtttcg agcgtactgg
     1261 catcgagcag cagtgcgctc ggaggctcag gctgagagat gcctgggatc tctgccaggg
     1321 gcgagccata gatcatcaac tgtgatccgt ccgaggtgaa ttccatcagg cgggggcgga
     1381 tggccaaggc ttgcttgccg atgaactgcc cggtcccaga gtcgtagatc aggactgatg
     1441 tgcctgcccg gtcgttgagg gccacggcga accgcttgct gtcctgactg aagactagcg
     1501 gcgacgccca gcctttgaat ggcgagggag acgcatcgat gtgggctaag aggtccgccc
     1561 ccgcttctcg ccaggtctgc aaatcgatca gccgaatcga atcggcgctc ggctggcagc
     1621 tcgggtaacc ggcgtaggcc tcgcagaacg accccacccc ggtggtggct gccagcctgc
     1681 tcccatccgg agagagggct aagttcgggg cattcctggt actgtggctt acgtcgatgg
     1741 gcggtcggtc tgggaacaca tagcctgtca tcggatcaac aagcctaagt tcgtagcgcc
     1801 gttgctcgct attccaggcc acgagaggaa cgctcgcttc gagcgggaag agtgccggac
     1861 cctcggccgg ggagttctct gaggcggaac tctctgagaa acttcccggc ctgtccccgc
     1921 cggtctcttg gtagggcact gggagttcgg cctcgcggcg ggccgagctg gagctggcca
     1981 aattgcctgc agctggggct ggtggcgccg gctcctcgcc gatgacggcc gatgagctgc
     2041 aggcggtcag caggatcact agggtcgtca ggaaaatcgc tcgcatggat acccccttgg
     2101 ggaagtaacc ctctcttcac ctaaatatac cccgctcgag cccagcaggt tccggagcgg
     2161 cgctcgccgg ccgaacagcc cctagtccgg ctttattccc aattcctgag cgagcttcgc
     2221 tggaatacag aaataggtaa cgctgatcga gggattcttg tgcgtcggcc ttcgataggg
     2281 atagatgctt ctgctgcggt acgagaattc atccaagaag acatatccgg caagttcgcc
     2341 gcatttgcgc cagctccacc tttgcgctcc gtgctcgcct tccgcaggag ggcgcaggcc
     2401 ctgccctgac atctcgtgta cgtaacggaa cccggcatga taagtgttcc aggcggtgtc
     2461 aagcagtgcc tcgtcgtcca gtgccgattc ttccgtgaaa atcaacacaa aacctgcgtg
     2521 ctcatatccg cggaagacct cgaccatctt ctcgttggtc tgattgcgta tctcatgaaa
     2581 accgttccct accatcatca ccgtcggctc accggccagc tcctgtcggg cgaggtactg
     2641 gatcactcca ctcggctcgc caatatccgc ggaccgaatg aactgcaggc tggcgggcaa
     2701 aatcccttgc tcctgcaagc caatcgcttg atcgatcgcg gtatcttcca ggtcggctgc
     2761 aaaatacttc gcctgctgct ccccgtcccg caggtatcgc tgccggatcg cctctccgcg
     2821 cccggcc
//
LOCUS       DATDPQ010000253         6933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3800246, whole genome shotgun sequence.
ACCESSION   DATDPQ010000253 DATDPQ010000000
VERSION     DATDPQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3800246"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="tyrS"
                     /locus_tag="VI520_05660"
     CDS             <1..994
                     /gene="tyrS"
                     /locus_tag="VI520_05660"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLF37403.1"
                     /translation="LIGRFTSLVGDPSHEDKVRAQISMEQATANAETYARQAFKILDP
                     DLTLIRYNDEWLSPLTFADLVRLAANFTVQQFLSRESFRQRFDSDDPIYLHEFMYSLM
                     QAYDAYHMKTDVQIGGTDQLFNILTAGRKLMEALGAQPNIGVILAILPGTDGEIRMSK
                     SLGNHIPLNSSAADMYGKVMSVPDKAMGVYFRLATRWVPSVISQIEAELSSGTGHPRD
                     IKMKLAKEIVEIYHGQEAAAAAESEFRQIFQQGAEPEEIPGYILSGPTRLVDVMLASG
                     LAGSRGEARRLVQQGGVRLNDEVLEDANLELGSAAGGVLRVGKRRFLRLVAPDS"
     gene            complement(1008..1445)
                     /locus_tag="VI520_05665"
     CDS             complement(1008..1445)
                     /locus_tag="VI520_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37404.1"
                     /translation="MATVIAAGGFLLLFCVPLASGSSQAIFQSKVAPAVQGRVFAIRT
                     LISRSMLPLAYASAGPLADRLFEPMLRDPASALSRTIGAVIGTGPGRGMGLMFILGGV
                     ALLAATAAAYANPRIRHIEDELPDAISSIPETALAGGAVAAEA"
     gene            complement(1481..1768)
                     /locus_tag="VI520_05670"
     CDS             complement(1481..1768)
                     /locus_tag="VI520_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37405.1"
                     /translation="MQDSFHSLQQDIAYGFKYLRGRRGLLGLLIYFALVNFTLNSTAV
                     LSGPLVLSTSDAGALGIVQTAMGAGMLIGSIVMSAWADPAGGSMASWGSLG"
     gene            complement(1784..1990)
                     /locus_tag="VI520_05675"
                     /pseudo
     CDS             complement(1784..1990)
                     /locus_tag="VI520_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="modification methylase"
     gene            1994..2066
                     /locus_tag="VI520_05680"
     tRNA            1994..2066
                     /locus_tag="VI520_05680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:Ser,seq:gga)
     gene            2245..3618
                     /locus_tag="VI520_05685"
     CDS             2245..3618
                     /locus_tag="VI520_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37406.1"
                     /translation="MSALVEALSLQRDQILKSAAGAPSYDGQFGPQVNALAADAAAQV
                     HSRFTRLGELARELEAIAELFETADAETLQAIEGIGIQIESEIAGFMALLARLHPPGG
                     PPPPLRLDLTLEEFQAMSAAERIAWVESFNEGTGAGWFSNFEDILHYFNDSQIFSGLD
                     GSTPASLWMSWGDAAVLQVVQDGYALFRDPDAKLPDMPAAPSTATADQRLAAARLWAD
                     FFDANETDPRRLPTLRRIWGEAEQAGVDYGQALADDQLELKSIQLSQSEAEIVEVFVG
                     FGDAYRTTIANDAGRAVFEAAPGAFVGEVAHQVAQEVQGSVQEEVRQRLEPAGGLFEL
                     FTGQPGNLLIDQVCGVLFGEDTLIGQTDEVAETFGEWAGDRMATRLEPQLGDLGDQFF
                     DPRLRLGIAVEFASPVGEVELDIGVRGPLYHLSMTVEEMLLRGDGDLANSFLDSVQAA
                     VEEALGR"
     gene            3640..4680
                     /locus_tag="VI520_05690"
     CDS             3640..4680
                     /locus_tag="VI520_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37407.1"
                     /translation="MKAQALRTTAQDLILLAGFPLAAVACAGPLRSPGPSEMPETPEM
                     AQLNYIQSPSGLYGESIWLDDETLAVQFRTGFLGALSTRIWRLSLNGRSFEEIELPKH
                     PSCGSQGQNGFEVPSRLPDGRMGYIVGCAVDSTSEIKRFVMALNLDTGEVEQLLEYPL
                     PAFRVGSGGFEWNPTMDVGIMGDGRRYIEEQLYWYTREGTEPIDVGLVQAYGPTWSPD
                     GEQIAFIGSKERGSPLTYSNLGLYLMSAEGKQVRPVLEGFHDASGVSWSPQGRWLAFP
                     GAFGEREAETYGLWLFDLERNQVRQIAEGVFGLPRWSADGQRIAVVQFLGPLDDPEDR
                     VAIVEVSPLLGD"
     gene            complement(5039..6505)
                     /locus_tag="VI520_05695"
     CDS             complement(5039..6505)
                     /locus_tag="VI520_05695"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLF37408.1"
                     /translation="MSERRQVRKQDFKKLSPFLWELPTSYRPGMRVPVRLFADDRLLE
                     DALDDKSLEQAVNASMLPGLVGHVTVMPDVHQGYGFPIGGVAATRLPSGVISPGAIGY
                     DINCGVRLLASSITLEEAQPQMADLASALYRNCPSGVGVKGSVKLSTKELDRVCREGS
                     EWALDNGYAQELDLARTEEGGRISGADPAAVSDRAKQRGQPQLGTLGAGNHFVEVDVV
                     DQIFDPAAATAMGLVAGTLAVQIHCGSRGFGHQVCTDYVREFQPAAARYQIQLPDREL
                     VCAPLDSPEGKRYLAAMACAANYAFANRQIMAHHVRRSFEEVLGGRVSNWELHQVYDI
                     AHNMGKIEEHAVAGDRMRVCVHRKGATRAFGPGFSGLPDEYRPLGQPVLVPGSMGTAS
                     WVLLGTEASMEQSFGSTCHGAGRTMSRKQAKRTVRGDQLRAELERRGIQIRAGSMPGL
                     AEEAPQAYKDVDAVVEVVAKAGIAKKVARLRPVAVIKG"
     gene            complement(6498..>6933)
                     /locus_tag="VI520_05700"
     CDS             complement(6498..>6933)
                     /locus_tag="VI520_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="archease"
                     /protein_id="HLF37409.1"
                     /translation="GGLGYTDPMPSGGFEELEHTADWALRVRGRDLAELLVNAANGMM
                     ELAGVQTASAAGTIRKLELTSLDRESLLVDWLHELLLALELRSVAFKQIRLKVTGDLG
                     LTATFREVPLTSLAKPIKAVTYNELQIEESPQGLFATVVFDV"
BASE COUNT         1327 a         2133 c         2152 g         1321 t
ORIGIN      
        1 cctgatcggg cgtttcactt cgctggtggg cgacccctct cacgaagaca aggtgagggc
       61 ccagatcagc atggagcagg ccactgccaa cgcggagacc tacgcccggc aggccttcaa
      121 gattctcgat ccagatctca cattgattcg atacaacgat gagtggctga gcccgctgac
      181 cttcgccgat ttggtccgtc tggctgccaa tttcaccgtt cagcagttcc ttagccgtga
      241 atcattccgg cagcggttcg actcggatga cccgatctac ctgcacgaat tcatgtattc
      301 gctcatgcag gcctacgacg cgtatcacat gaagaccgat gtgcagattg gtggaaccga
      361 tcagctcttc aacattctga ctgccggccg caaactgatg gaagccctcg gcgcgcaacc
      421 caatatcggc gtcattctgg cgatactgcc aggcaccgac ggggaaatcc ggatgagcaa
      481 gtcgctcggc aaccatattc cgcttaacag ctccgcggcg gacatgtatg gcaaagtcat
      541 gagcgtgccg gacaaggcca tgggtgtgta ttttcgcctg gcgacccgct gggtgccgag
      601 cgtgatttcg caaatcgaag ccgagctgag ctccggtacg ggccacccgc gggacatcaa
      661 gatgaagctc gccaaggaaa tcgtcgagat ctaccacggt caggaagccg ccgcggcggc
      721 cgagagcgag tttcgccaga ttttccagca gggcgcggaa cccgaggaga ttcccggata
      781 cattctgagc ggccccactc ggctggtaga cgtgatgctt gcatccggcc tggcgggcag
      841 ccgcggtgag gcccgccggc tggtgcagca gggaggggtg cggctcaacg acgaagtgct
      901 ggaggatgcc aatctggagt tggggagcgc ggccggtggc gtcctccgag tcggtaagcg
      961 ccgctttcta cgcttagtgg cgccggactc ctgatcaggt tgccgggcta agcttcggca
     1021 gccaccgctc cgccagccag agccgtttcc ggtattgatg agatcgcgtc cggaagttcg
     1081 tcctcgatgt gccggatgcg gggattggca taggccgccg cagtggcggc cagcaaggct
     1141 acccctccca agatgaacat cagccccatg cctctccccg gtccggtgcc gattaccgca
     1201 ccgattgtcc gagagagtgc tgacgccgga tctcggagca tgggctcgaa tagtctatcc
     1261 gccaggggtc ccgcgctggc ataggccagg ggcaacatgg accgtgagat cagggtgcga
     1321 atagcgaaca ctcggccctg aactgcgggc gcgaccttgg actgaaagat cgcctgactg
     1381 gatccggatg ccagtggaac gcagaacagg agcaggaatc ctccggcggc gatcacggta
     1441 gccaacgggt gcagcccggc gatcgccagt ccaattcctc ctaacccaat gaaccccagg
     1501 acgccataga tccgccggcg gggtccgccc aggcgctcat gacgatgctt ccgatcagca
     1561 tccccgcccc catcgcggtc tgcacgatcc ccaaggctcc agcatcggac gtactgagaa
     1621 ccagcggacc gctcaatacg gcggttgagt tcaacgtgaa gttcacaagc gcgaagtaga
     1681 tcagaagtcc gagcaatccg cggcggcccc gcagatactt gaatccataa gcgatgtcct
     1741 gctgcagcga gtggaatgag tcttgcaagc ctttcgccgc ctgtcaaccg ggcgaataga
     1801 aattgggact tgtctcgggg aaagcaggat ccgacggctg gcggcgaggc cggaaatcga
     1861 ggaattgtgc attgggcgga tctggcacga tggcctcaat ccagcggcga gcgatctcgg
     1921 caaagccggg ctccttctca atgccaatcc actgccgccc taagcgcttg gcgaccgctc
     1981 ccatcgtgcc ggtgccgaaa tagccgagtt agtcgagcac gcgactggaa atcgcggtgt
     2041 cgccagttcg attctggctc tcggcacctc agtctgatca actcctcagc gacacccgca
     2101 cggccgagct ctgggccaaa agccatctat aatctagctg agcactcgcc tcggcgccgg
     2161 tgtagcgtta gggccgggtc ggcaagctgc atgtcgcgcc tgattcaagt tgatccccag
     2221 aatctccgag cctccgccca gcggttgagc gcgcttgtcg aggcgctttc tctccagcgg
     2281 gaccaaatcc tcaaatcggc cgcgggggcg ccaagctacg acggtcagtt cgggccccag
     2341 gtgaatgcgc tcgcagccga cgcagccgcc caggttcaca gcagattcac ccggttgggc
     2401 gagctggccc gcgaactcga agcaattgcc gagctctttg agaccgcgga cgccgagacg
     2461 ctgcaggcga ttgaagggat cgggatccag attgagtccg agatcgccgg cttcatggcc
     2521 ctgctggctc ggctccatcc ccccggcggc cctccgccgc cgctgcggct cgacctcacc
     2581 ctcgaggagt ttcaggcgat gtcggcggcg gagcggatcg cctgggttga gtctttcaac
     2641 gagggaaccg gagcaggctg gttttccaac tttgaagaca tccttcacta tttcaacgac
     2701 tctcagattt ttagtggcct tgatggttct acgcccgcta gcctgtggat gtcctggggc
     2761 gacgccgcgg tgctccaggt cgttcaagat ggctacgccc tgtttaggga tcctgacgcg
     2821 aaacttccag acatgccggc agccccctcg actgcgacag cggatcaacg ccttgcggcc
     2881 gcgcgactgt gggcagattt ctttgacgcc aacgaaaccg accccaggcg tctgcctacc
     2941 ctcagaagga tttggggtga agccgaacaa gccggagtgg actacggcca ggcactggca
     3001 gacgatcaac tggaattgaa atccattcag ctgagccaat cggaggcaga gatcgttgaa
     3061 gtgtttgtcg gtttcggcga cgcgtatcga acaacgatcg ccaacgacgc cgggcgcgcc
     3121 gtcttcgagg cggcaccggg cgcgtttgtg ggtgaggtag cacaccaagt tgcccaggag
     3181 gtacagggct cagtgcaaga ggaggttcga cagagactcg agcctgccgg tggcctgttt
     3241 gaactgttca ccgggcagcc agggaacctt ctcatcgatc aggtctgcgg agtattgttc
     3301 ggagaggata ccctgatcgg ccagaccgat gaagttgcgg aaaccttcgg agaatgggca
     3361 ggggatcgaa tggccactcg gcttgaaccg cagcttgggg atctgggtga tcagttcttc
     3421 gatccccggc ttcgactcgg gatcgcggtc gagttcgcca gtcctgtagg agaagtcgaa
     3481 ctggatatcg gggtcagagg gccgctttac catctctcga tgacagttga agaaatgctg
     3541 ctaagaggag atggcgatct ggccaattcg ttcctcgatt cggtccaagc tgcagtagaa
     3601 gaggccctcg ggcgataatg tgaatgcagg tggtgtggat tgaaagccca agctctacgg
     3661 actacggctc aggacctgat cctgctggcc gggttcccgc ttgccgcagt ggcctgcgca
     3721 ggcccattgc gttcgccggg cccatccgag atgcctgaga ctccggaaat ggcacaactg
     3781 aactacatcc agagcccctc gggattatac ggcgagtcca tttggctgga tgatgaaacg
     3841 ctggcagtcc agtttagaac cggcttcctt ggcgccctca gcactaggat ctggcgcctc
     3901 agcctcaatg gccgatcttt cgaagaaatc gaacttccga aacacccttc ctgcggatct
     3961 caaggccaga atggtttcga ggtacccagc cgtctgccag atggacgcat ggggtacatt
     4021 gttgggtgcg cggtggacag cacatccgaa atcaagcggt tcgtaatggc tctcaacctc
     4081 gataccgggg aagtcgagca gcttctggaa tatccgctgc cagcgtttag ggtggggtct
     4141 ggcggcttcg agtggaatcc caccatggac gttggcatca tgggagacgg tcgtcgatac
     4201 atcgaggagc agctctactg gtatacgcga gagggcaccg agccaattga tgtcgggctg
     4261 gttcaagcct atgggccaac ctggtcgccc gacggtgagc aaatcgcttt tattggctcg
     4321 aaggaacggg gttcaccgct cacgtactcg aacctgggac tttatttgat gagcgcggag
     4381 ggcaagcagg tccgtccggt actcgaaggg ttccacgacg cgagcggagt ttcctggtcc
     4441 ccgcagggcc gctggttggc ctttccggga gctttcggcg agcgagaggc agagacctat
     4501 ggtctgtggc tcttcgactt agagcgcaat caggtgcgcc agattgcaga aggggtattc
     4561 ggcttacctc gctggtccgc cgatggtcaa cggatagccg tggtccaatt cttggggccg
     4621 ctagatgacc ctgaggaccg ggtggcgatc gtcgaggtca gtccgttgct gggggactag
     4681 tcgctgcagc tggaaacagc gatcgtgtta gagttggttc tttcccggta ccaccttcga
     4741 agctcattaa tgggcataag tcctccgggc tcggcctcgt aatactagtg ctcccacccg
     4801 ttgcacgggc cgccgccggc caaatggctg cccagctggt ggttcggtcc ctcgatcccg
     4861 ttcggtttca gctggccatc ggttccctct taagccgacc gccttcgatc cttcttgaaa
     4921 tacagcttct agccggggag gatcaaggct gcttcgagca gcgaggcaaa tgggagccgc
     4981 gaccggggtt tggcgagttg ggaggtcggg aggaagggca gacgaggttg ccggcagctc
     5041 agcccttaat gaccgccacc ggccgcaggc gggcgacctt cttggcgatc ccggctttgg
     5101 cgaccacctc gacgaccgca tcgacgtcct tgtaggcctg aggggcttct tccgcaagcc
     5161 cgggcatcga acccgcccgg atctggattc cacgcctctc aagctcggcc cgcaactggt
     5221 cgccacgcac agtcctcttc gcctgcttac ggctcatggt ccgcccggct ccgtggcagg
     5281 tcgagccgaa agactgctcc atgctggctt cggttccgag cagcacccag gaggccgtgc
     5341 ccatgctgcc ggggaccagg acgggctggc cgagcggacg atactcgtcg ggcaaccccg
     5401 aaaatccggg gccgaaggcg cgggtcgccc ccttgcggtg cacacacacc cgcatccggt
     5461 cgccggcaac ggcgtgctcc tcgatcttgc ccatgttgtg ggcgatgtcg tagacctgat
     5521 gcagctccca gttgcttact ctgcccccta ggacctcttc gaacgaacgc cgcacatggt
     5581 gggccatgat ctggcgattg gcgaaagcgt agttggcggc gcaggccatg gcggccaggt
     5641 agcgcttgcc ctccggcgag tccagcgggg cgcagaccag ctcgcgatcc ggaagctgaa
     5701 tctggtagcg ggcggcggcc ggctggaatt cccggacgta gtccgtgcac acctggtgcc
     5761 cgaatccccg tgagccacag tggatctgca ccgccagagt tccagccacc aatcccatcg
     5821 cagtggctgc cgcagggtcg aagatctgat cgacgacatc gacctcgacg aagtgattgc
     5881 cggcgccaag ggtgccgagc tgcggttgac cgcgctgctt cgcccggtcg ctgaccgctg
     5941 ccgggtcggc gcccgatatt ctcccgccct cttcggtcct ggcgagatca agttcctgcg
     6001 catagccatt gtcgagcgcc cactctgagc cctcgcgaca cacccggtcg agctctttcg
     6061 tgctgagctt gacactgcct ttcactccga ccccgctcgg gcaattgcga tagagggcgg
     6121 aggccaggtc cgccatctgg ggctgcgcct cctccagggt gatcgaacta gccagcagcc
     6181 gtacgccgca gttgatgtcg tacccgatgg cccccggtga aatcaccccg cttggaagcc
     6241 gggtggctgc cacgcctccg atcgggaatc catagccctg gtgcacatcc ggcatcacgg
     6301 ttacatgacc gaccaggccc ggcagcatgg atgcgttgac cgcctgctcg agcgacttgt
     6361 cgtccaatgc gtcctcaagc agacgatcat ccgcaaagag gcggaccgga acccgcatgc
     6421 ccggacgata cgaggtcgga agctcccaaa ggaagggcga cagcttcttg aaatcctgtt
     6481 tgcgaacctg gcggcgctca gacatcgaaa acgaccgtgg cgaacagtcc ttgcgggctc
     6541 tcttcgatct gcagttcgtt gtaggtgacg gccttgattg gcttggccag acttgtgagc
     6601 ggcacttccc ggaacgtggc agtcaagccg agatccccag tgaccttcag tcggatctgt
     6661 ttgaaggcga cgctccgcaa ctcgagcgcc agcaatagct cgtgcagcca gtcgaccagc
     6721 agcgattccc ggtcaaggga ggtcagctcg agcttccgga tggtcccggc cgccgaggcg
     6781 gtctgaacgc ctgccagctc catcattccg ttcgccgcgt tcaccagcag ctcggccaga
     6841 tcccgcccgc gtactctgag cgcccagtcg gcggtgtgct ccagctcctc aaaaccgccc
     6901 gacggcatag ggtcagtata tcccagccct ccc
//
LOCUS       DATDPQ010000254         4329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2225459, whole genome shotgun sequence.
ACCESSION   DATDPQ010000254 DATDPQ010000000
VERSION     DATDPQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225459"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1457
                     /locus_tag="VI520_05705"
     CDS             81..1457
                     /locus_tag="VI520_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLF37410.1"
                     /translation="MPTNWQAIDRAIDDRLERTLAELGKLCAQPSVSAHGLGMQECAE
                     LVAEMLQARGFDTKVMATDGQPVVFGERSGRGDRTLLLYNHYDVQPPEPLDLWITPPF
                     EASRRDGQLFARGVSDDKGHFQCRLAALDAFLEVEGELPCSVKFVVEGEEEVGSVHLP
                     EFVKRHSGLLAADACLWEFGEINHEGTPIQAAGMRGICYVELSVETASRDSHSGTSGS
                     IFPNAAWRLVWALRTLKGSDEMIRLPGFYDRVVPPTERDLALLEQIPDPSDHFREFYG
                     IKRFLKGTEGGLDLLREQVFVPTCTICGLTAGYQGPGSKTVLPARASAKVDFRLVPDQ
                     RPSEVLEQLRAHLDAEGFPEVEILELGSEPPGRTDPDDPFLQLVVNSAVEVYGVPQLI
                     SPMVGGSGPNHAFIHGLGVPVATAGLGYPGSNVHAPNENIVIDYFVRGARHTARVLGY
                     FGAGLTLP"
     gene            1493..1567
                     /locus_tag="VI520_05710"
     tRNA            1493..1567
                     /locus_tag="VI520_05710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1528..1530,aa:Pro,seq:ggg)
     gene            1647..2375
                     /locus_tag="VI520_05715"
     CDS             1647..2375
                     /locus_tag="VI520_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLF37411.1"
                     /translation="MKVQLFATCLVDSLFPEVGEAVVEVLAKAGVEVWFPKGQTCCGQ
                     PALNAGMRAEARTLALHTLEALEAEDVVVVPSGSCSEMIRHRYPELLADEPGQLKRAR
                     RLADRTYEFSEFLVDQLLVADFGAALPLVIGYHPSCHLLRGLGVDRQPLRLLEGIAGA
                     STERLSPECCGFGGMFSIEMPEISAEMLERTLDRIRSSGIELVAGCDVSCLMQIEGGL
                     RRAGSSVRCAHLAQLLAGREAGLK"
     gene            2372..>4329
                     /locus_tag="VI520_05720"
     CDS             2372..>4329
                     /locus_tag="VI520_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HLF37412.1"
                     /translation="MTLAFRQRARQAIADPQLQRLLDQNADVRQQARMEALEELPQSG
                     ELRGRARSIRQQVIAELGAYLDQFGTRLAENGIQAQRAANAAEANRLILDIVQKHKAT
                     LVAKSKSMLTEEIGLNAALEAAGIRPVETDLGEYVVQLRAEKPAHILTPALHLSTQDV
                     AETFRRQLGASLGDDVDQINAEARRQLREVFLTAPVGITGVNFGVAETGTLCLVTNEG
                     NGRMVATLPPVHIAVMGIERLVPTLDDLAVMLRLLPRFATGQAITSYVSLIHAPRRAG
                     DPEGPTERHLILVDNGRSELASTAAAESLLCIRCGACLNACPIFREAGGHAYDSVYSG
                     PIGAVLSPGLFGIEKYGHLGKASTLCGACTEACPVGIDIPGLLLNVRAEVEPKPLSRG
                     LQLFSWVARSPTRFRWAQRTAALGGRLLARRAGWITQLPGPFAEWTHFRDFPPFASRP
                     LRSRLRRSRRPAKDRLPELEPEEAAPAVAQAAGEPDDRTDRFDAKLKALGGEVIRCAE
                     FEAADRIVGQLYVLGASRLLSWGPVEPILFTVLQRLEREGFPVVQPEVPEGAGGTRPI
                     RAFGEFPVGLTGAVAGLADTGSLILSGGSRRSLLPSLLPETHLAVVRSKDIYADLEGW
                     LRAVGSPYVAGSSNLVLISGPSRTADIE"
BASE COUNT          729 a         1337 c         1454 g          809 t
ORIGIN      
        1 atccgcatgg acggcggcgg attatagagt cgatcccaac agaatgcgaa ccgggtcttt
       61 cccgcgacta taatcggccc atgcccacca attggcaggc gatcgatcgc gcaattgatg
      121 accggctcga gagaactcta gccgagctgg gcaagctgtg cgcccagccg agcgtctcgg
      181 cccacggatt gggaatgcag gaatgtgccg agctggtggc cgagatgctg caggccagag
      241 gcttcgacac caaagtcatg gcgaccgacg gacagccagt cgtctttggc gaacggtccg
      301 gccggggcga ccgaactttg ctgctgtaca accactatga tgtgcagccc ccggaaccgc
      361 tggatttgtg gatcacacct cctttcgaag ccagccgacg cgacggccag ctatttgccc
      421 gcggggtgag cgatgacaag ggccatttcc agtgccggct cgcagccctg gacgctttcc
      481 tggaagttga aggggagctg ccctgttcgg tcaagttcgt cgtcgaagga gaggaggagg
      541 tcgggagcgt ccatctgcct gagttcgtca agcgccattc cgggctgctg gcggccgacg
      601 cttgcctgtg ggagtttggc gagataaatc acgaaggtac gcccattcaa gctgctggaa
      661 tgcgcggcat ctgctatgtc gaactcagcg tggagacggc atcccgcgac tcgcactcgg
      721 gcacgagcgg ctcgatcttc cccaacgcgg cctggcggct ggtctgggcg ctccggaccc
      781 tcaaagggtc ggacgagatg atccggctac ccggctttta cgatcgggtg gtaccgccga
      841 ccgaacgcga tctggcgctg ttggagcaga tccccgaccc gagtgaccat ttccgggagt
      901 tctatgggat caagagattc ctaaaaggaa ccgagggcgg gctcgacctg ctgcgtgagc
      961 aagtcttcgt cccgacgtgt acgatctgcg ggctgacggc cggttatcag gggccgggga
     1021 gcaagaccgt tctccccgcc cgggccagcg ccaaggtaga ctttcgccta gtaccggatc
     1081 agcggcccag cgaggtcttg gagcagctgc gggcccattt ggacgcagag ggcttcccgg
     1141 aggtcgagat cctcgagttg ggcagcgaac cacccggccg cacggatccg gacgatccgt
     1201 ttttgcagct cgtcgtaaac tcggcagtcg aggtctatgg tgtcccccaa ttgatcagtc
     1261 cgatggtggg cggctccggc ccgaatcacg cattcatcca tgggctcggc gtcccggtgg
     1321 cgaccgccgg cctcggttac cccggctcca acgttcatgc gccaaatgag aacatcgtga
     1381 ttgattactt cgtccggggg gcacgccaca ccgccagagt tctcggttat ttcggcgcgg
     1441 ggctgaccct tccgtagccg agcgggcaca tctataatcg atccggtatc ctcggggcgt
     1501 agcgcagccc ggttagcgca cctgcatggg gtgcaggggg tcggcggttc gaatccgccc
     1561 gccccgactg cggaccgtcg caacttcgcg gcggtcccgt cgtttcacac cccgttcaaa
     1621 agaaccgaat gttttcaggg cgacggatga aggtccagct attcgccacc tgtttggtcg
     1681 atagcttgtt tcccgaggtc ggggaagcgg tcgtcgaggt cctggccaag gccggagtcg
     1741 aagtctggtt tcccaagggc cagacttgct gcgggcaacc ggccctcaac gccggaatgc
     1801 gggccgaggc gcgcacgctg gcccttcaca ccctggaggc cctggaggcc gaggacgtcg
     1861 tcgtggtccc ttcgggttcc tgttccgaga tgatccggca ccgctatccg gagctcctgg
     1921 ctgacgaacc cggccagctg aagcgggccc ggcggttggc ggaccgcact tacgagttca
     1981 gtgaattcct ggtcgatcag ctgctggtcg ccgatttcgg tgccgcgctc ccgctcgtga
     2041 ttggatacca cccctcctgc cacttgctca ggggattggg agtggaccgc cagccgcttc
     2101 gactgctgga aggaatcgcg ggagcttcga ccgagcgcct gagtccggag tgctgcgggt
     2161 ttggcgggat gttttcgatc gagatgccgg agatctcagc cgagatgctg gagcggaccc
     2221 tcgatcggat tcgaagttcg gggatcgaac tcgtggcggg atgcgatgta agttgtctga
     2281 tgcagatcga aggcggcctg cggcgggccg gctcctcggt gcgctgtgcc catctggctc
     2341 agctcttagc tgggcgagag gcggggctga aatgactttg gctttccgac agcgtgctcg
     2401 ccaggcgatc gccgatcccc agctgcagcg actgctggat caaaatgccg atgtccgcca
     2461 gcaggctcgg atggaagccc tcgaggagct gccccaatcc ggcgagctgc gtgggcgggc
     2521 ccgctcgatc cgccagcaag tgatcgccga gctaggcgcc tatctggatc agtttgggac
     2581 ccgcttggcc gagaacggga tccaagccca gcgggctgcc aacgcggcag aggctaaccg
     2641 tttgatcttg gacatcgtcc aaaagcacaa ggccacgctg gtagccaagt ccaagtccat
     2701 gctgaccgag gagatcggct tgaacgccgc cctcgaagcc gccgggattc ggccggtcga
     2761 aaccgacctg ggcgagtacg tcgtccagct gcgggctgag aagccggctc acatcctgac
     2821 ccccgccctg cacctaagta ctcaagatgt cgccgaaact ttcaggcgcc agcttggcgc
     2881 gagcctgggt gacgatgtgg accagatcaa tgccgaagcc cgccgccagt tacgggaagt
     2941 cttcctgacc gctccggttg ggattacggg cgtgaacttt ggggtcgccg aaaccggaac
     3001 gctgtgcctg gtgacaaacg aagggaacgg tcggatggtc gccactttac cgccggtgca
     3061 tatcgcggtg atggggatcg agcgccttgt cccgaccctg gacgacctgg cggtgatgct
     3121 gcggctcttg ccacggttcg cgaccggcca agcgatcacc agctacgttt cgctcattca
     3181 cgctccccgc cgggctggcg atcctgaagg cccgaccgag cggcacctga tcctggtgga
     3241 caacggccgt tcagagctgg cgagcactgc ggcagccgag tcgctgctgt gcatccgctg
     3301 cggcgcttgt ctgaacgcct gcccgatctt tcgcgaggct ggcggccacg cgtatgacag
     3361 cgtgtactcc ggaccgatcg gagccgtgtt gtcgcccggg ttgttcggaa tcgagaagta
     3421 cgggcatctc ggcaaggcct cgactctctg cggcgcctgc acggaggcct gcccggttgg
     3481 aatcgacatc cccggcttgc tgctgaacgt tcgggctgaa gtcgagccga agccgctcag
     3541 ccgcggcctc cagctgttca gttgggtggc ccggtcgccg acccgcttcc gctgggccca
     3601 acggacggca gcactgggcg gccggctgtt ggcgcggcga gccgggtgga tcactcagct
     3661 ccccggaccg ttcgcggagt ggacccactt ccgcgacttc ccgccgttcg cctcccggcc
     3721 gctccgttca cggcttcgaa ggtcgagacg gccggccaag gatcgcctac ccgaacttga
     3781 gccggaggag gcggcacccg ccgttgctca ggcggccggc gagcccgacg accggaccga
     3841 tcgatttgac gcgaagctta aggccctagg cggagaagtg atccgctgcg cggagttcga
     3901 ggctgcggac cgaatcgtcg gtcagctgta tgtgctggga gccagccgat tgttgtcgtg
     3961 gggaccggta gagccgattc tatttactgt gctgcagcgg ctggagcggg agggttttcc
     4021 ggtcgttcag ccggaggtac cggagggcgc cggcgggacg cggccgatcc gtgcgtttgg
     4081 tgagttcccg gttgggttga ccggggccgt ggccggtctc gcagacaccg ggtcgctgat
     4141 cctctcggga ggttcgcggc ggtcgctgct gccctcgctg ctgcccgaaa cgcacttagc
     4201 ggtcgtgcga tcgaaagaca tctatgctga tctggagggg tggctgcggg cggtcggctc
     4261 tccctatgtg gccgggagtt ccaatctagt gttgatcagc ggaccttccc gaacggccga
     4321 tatcgagat
//
LOCUS       DATDPQ010000255         4310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1953970, whole genome shotgun sequence.
ACCESSION   DATDPQ010000255 DATDPQ010000000
VERSION     DATDPQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953970"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VI520_05725"
     CDS             <1..1553
                     /locus_tag="VI520_05725"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HLF37413.1"
                     /translation="REYITIAPDAEIEKAVLTWGAERLAALTAATYAKSEFYDAMRGL
                     EEEAAAALPELSPADLREVLWSRVKAGVRERVLKEGVRVDGRGPDDLRKIEVQVDLSP
                     RAHGSGLFNRGETQVLTLATLGTPRESQELDILAPVELKRYMHHYNFPPFSTGETKFL
                     RGASRRDIGHGALAERALLPVLPSKDEFPYTMRLVSEVLSSNGSTSMASVCGSTLALL
                     DTGVPIKAPVAGIAMGLVKDGDRYRILTDIQGLEDQLGDMDFKVAGTREGVTALQMDI
                     KTKGLTPELLSEALERARQARLSILDKIEELMPRPRAELKPHAPRMTVLHIDPQKIGA
                     VIGPGGKMIRGIQEMTGARIDIEDDGTIYIASAEGPGAEEARRRIEALVEEAVVGRIY
                     TGKVVRTTEFGAFVEILPNTDGLVHISQLATEHIEQVEDVARVGDELTVMVTDIDLAG
                     KVRLSRTAVLKGWTLEEAMEHDQRPRRSSSGGDRGRGGDRGRGGDRGRGGRGPGSGDR
                     GRPSGPRR"
     gene            1609..2850
                     /locus_tag="VI520_05730"
     CDS             1609..2850
                     /locus_tag="VI520_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLF37414.1"
                     /translation="MQDPGSTRPQRASSPDWQPGTRLAVGVLMVVLAALLLYRLRQLL
                     TPVILAFLLAYILYPLVRRLEDRLRSSRGIAVGLVYLIILLLLGGTTTGIGIALSQLL
                     AGFGQFLERISQQLPEFVDSLMVLQFQLGPLVIDLSSVEGLANSLTSAIAPLVSQTGT
                     LLSDVAGATASFVGNALIVLVIGFYVLLNFERIRDWLIAAAPAPYQADARALLSGTGV
                     IMQAFLRGQLILGLVVGAAMAVTMTILGVRYALGIGLIAGVLEFIPIFGALVTAIISV
                     LVALFQGSNGWGLTPFGFALVVAGASILIQQIENNVLVPRIIGHSLDLNPLVVILALL
                     AAGSLAGVVGLLLAAPAVATLKLAFSYLYWKTVGVQPAIAHTPAGPSAARPSRLRRLW
                     GRFRAGGRSKATPTPGSTPPT"
     gene            2955..3461
                     /locus_tag="VI520_05735"
     CDS             2955..3461
                     /locus_tag="VI520_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLF37415.1"
                     /translation="MTQRTELISWEEVGQLIEHLTPQFETEFDAMVIITRGGIVPGGM
                     LCEALNILDVLTAAVDFPFESQKSGSALYAWPAFIQFPDEDQLRGRQVLVVDDVWGSG
                     RTITAVKNRVSSAGGFPHTCVLHFNPYRSLFNEAKPDYYAAVTDAHVVYPWEVDRDAD
                     RVLLDAPE"
     gene            3692..>4310
                     /locus_tag="VI520_05740"
     CDS             3692..>4310
                     /locus_tag="VI520_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLF37416.1"
                     /translation="MDHSEEFRTLLTESISVPEANRGDLLQGRVIALDSYGIIVDLGL
                     KRDGLVPRADLELLPPAESKLKIGDQVSVMVVEPLDTDGNLVVSIAQARESGDWLRAQ
                     SLLESDAVVEAIVGGYNRGGLIIPFGRLRGFVPASHITDLPRGLDDNGQAEYMARLVD
                     KSLPLKVIEVDPRRRRLVLSERKAIRQWRQDQKARVIDQLALGEVR"
BASE COUNT          761 a         1302 c         1415 g          832 t
ORIGIN      
        1 agcgcgagta cattacgatc gcacccgacg cggagatcga gaaagccgtc ctgacttggg
       61 gggctgagcg gcttgcggcg ctcacggccg ccacctacgc caaatcggag ttctacgatg
      121 cgatgcgcgg cctcgaagag gaagcagccg cggccctgcc agagctcagc ccggccgacc
      181 tgcgcgaagt gctgtggagc agggtaaagg ccggggttag agagcgggta ctgaaggagg
      241 gggtcagggt cgacggccgg ggaccggatg accttcgcaa gattgaggta caggtggacc
      301 tcagcccccg cgcccatggg tcaggactgt ttaaccgggg agagacccag gtgctcaccc
      361 ttgccacgtt ggggactccc cgcgagagcc aggagctgga catcctggcg ccggtagagc
      421 taaagcgata catgcaccac tacaactttc cgccgttctc gaccggggag accaaatttc
      481 tgcggggcgc ctcgcgccgg gatattggcc atggggccct tgcggaacgg gcactcctgc
      541 cggtcctccc atcgaaggac gagtttccgt acaccatgcg gctggtttcg gaggtgcttt
      601 cttccaacgg atcgacctca atggcctccg tgtgtggttc gaccctggcc ctgctcgata
      661 ccggcgtgcc aatcaaggcg ccggtggccg ggatcgcgat gggtctggtc aaagatgggg
      721 atcgctaccg catactgacc gatattcaag gtctcgagga ccagctcggc gacatggact
      781 tcaaggtcgc tggaactcgg gaaggcgtca cggccctgca aatggacatc aagaccaagg
      841 gtctcacgcc ggagctcctg tcggaggccc ttgagcgagc ccgccaagcg cggttgtcca
      901 ttctggacaa gatcgaggag ctgatgcccc ggccccgagc ggagctcaag ccgcacgcgc
      961 cgcgcatgac ggtgttgcat atcgacccac agaagatcgg ggcggtcatc gggccggggg
     1021 gcaagatgat ccgcggaatt caagagatga ccggtgctcg aattgacatc gaggacgatg
     1081 gcacgatcta cattgcttcc gctgagggcc cgggggccga agaggctcga aggcggatcg
     1141 aagcgctcgt cgaagaagca gtggtcggcc gcatctatac cgggaaagta gtgcgcacga
     1201 ccgagttcgg tgcgtttgtc gagatcctgc cgaacacgga tggattggtc catatctctc
     1261 agcttgccac cgagcacatt gaacaggtgg aagacgtggc gcgagttggg gacgagctca
     1321 ctgtgatggt gaccgacatc gatctcgccg ggaaggtccg gctttcccgc actgccgtgc
     1381 tgaaagggtg gactttggaa gaagccatgg agcacgacca gcggccgcgg cgctccagta
     1441 gtggaggaga ccggggccgg gggggagacc gtggtcgagg tggagaccgt ggccgaggtg
     1501 ggcgcggtcc tggctcaggc gaccgaggcc gaccgtctgg acccaggcgg tagcagccgc
     1561 aactcaagcc cccggtaccc gggggctttt ttcttgtaca atcgccgaat gcaagatccc
     1621 ggatccacac gcccgcaacg ggcatcttcc cccgactggc agccggggac ccggctggcg
     1681 gtgggggttc tcatggtcgt gctggcggcc ttgctgctct accggcttcg ccagctgctg
     1741 accccggtca tcctcgcctt cctgctcgcc tacatcctgt acccgctggt ccgccgcctg
     1801 gaggatcgcc tgcgatcgag ccgcgggatc gcggtggggc tggtctactt gatcattctt
     1861 ttactcttgg gcgggaccac gaccggaatc gggatcgcac tatcgcagct cctggcgggg
     1921 ttcggccaat tcctcgagcg gatctcacaa cagctgcccg agttcgttga cagtttgatg
     1981 gtgcttcagt tccagttagg tccgttggta atcgatctga gcagcgtcga gggtctcgcc
     2041 aatagtctga cgtcggccat cgcgccgttg gtgagccaga ccggaaccct gctgagcgac
     2101 gtggcgggag ccaccgcctc atttgtcggc aacgccctga ttgtgttggt gatcggcttt
     2161 tatgtattgc tcaatttcga gcgaatccgg gattggctga tcgcggccgc ccccgctccc
     2221 taccaggccg atgcccgcgc cctgctgagt ggtaccggcg tcatcatgca ggcgtttttg
     2281 cgcggccagc tgatcctcgg attagtagtg ggcgccgcga tggccgtgac catgaccatc
     2341 ctcggggtgc gttatgcact tgggatcgga ctcatcgcag gggtattgga attcattccg
     2401 atctttggag ccctggtcac tgcaattatc tcggtcctgg tggccctctt ccagggatcc
     2461 aacggctggg ggctcactcc ctttgggttc gcactggtgg tcgcgggagc ttcaattctc
     2521 attcagcaga tcgagaacaa tgtgctcgtg ccgcggatca ttggtcacag cctggacctc
     2581 aacccgctgg tcgtgattct ggcgttgctg gccgccggca gcctggcggg agtcgtcgga
     2641 ttgctgcttg ccgctccggc ggtggcgacc cttaagctgg ccttcagcta cctgtattgg
     2701 aagacggttg gggtgcagcc ggctatcgct cacacccccg ctgggccgag cgcagcccga
     2761 ccctctcggc tgcgccgact atggggccga ttccgggcgg gcgggaggtc caaggcgacc
     2821 ccaaccccgg gctcgactcc gcccacatga cctcaatccg ggaagccctg cgccggctga
     2881 ttcagcgcga gcggttagcc tcgaccttat tgtctgggcc gggccgcagt gctatcatcg
     2941 gccagaggcg ggcgatgacc caacgaaccg agcttatcag ctgggaggaa gtcggccagc
     3001 taatcgagca cctcacccct cagttcgaga ccgagttcga cgcgatggtg atcatcaccc
     3061 ggggaggaat cgtcccgggt ggaatgctct gcgaggcgct gaatatcctg gacgtgctga
     3121 ccgcggcggt cgacttcccc ttcgagtcgc agaaatccgg ctccgcgttg tatgcctggc
     3181 cggccttcat ccaattccca gatgaggacc agctgcgagg tcggcaggta ttggtagtcg
     3241 acgacgtgtg gggctcgggc cgcacgatca ctgcggtgaa gaacagagtt tcatcagccg
     3301 gcggcttccc ccacacctgc gtcctgcact tcaacccgta ccgcagcctc ttcaacgagg
     3361 ccaagccgga ctactacgcg gccgtcaccg acgcccatgt cgtgtacccc tgggaggtcg
     3421 atcgagatgc ggatcgggtg ttgctcgacg cgccggagtg agcaggaacg agccgcgtcc
     3481 cgcccgcttc accgggttgt ggcctacaag acgcctccaa ttgacaaccc tgtgagcgcc
     3541 ctgtataatg accacgcgct tcgggctatt gctggcccgg gaccgtcact gaacgaagct
     3601 taggagtatt agggaaggat cgctcttaaa ctccttggga gggattgtgg cctggtcggt
     3661 ttctgagcgc tgatcaggct cttttttttg cgtggaccat tctgaagagt tccgtaccct
     3721 gctgaccgag tcaatctccg tcccggaggc aaatcgtggc gatttgctgc aaggccgggt
     3781 cattgcgctc gattcctacg ggatcatcgt ggacctcggt ctcaagcgcg acggcctggt
     3841 cccgcgggca gacctggagc tgctcccccc ggcggagtcc aaacttaaga tcggtgatca
     3901 ggtctcagtc atggtagtgg agccgctgga tacggacggg aatctggtgg tctccatcgc
     3961 tcaggcccgt gaaagcggcg attggttgcg agcccagagt ctgctggaaa gcgatgccgt
     4021 ggtcgaggcc atcgtgggtg gctacaaccg cggcggtttg atcataccct tcggtcggct
     4081 gcgcggtttc gtccccgcct cgcatatcac cgatctgcct cgaggcctgg acgacaacgg
     4141 gcaggccgaa tacatggcgc gcctggtcga caaatctctc ccgctgaagg tgattgaagt
     4201 cgatccacgc cggaggcggc tggtgctctc ggagcgcaag gcgatccggc agtggcgtca
     4261 agaccagaag gcccgggtga tcgatcagct ggcgctcgga gaggtccgag
//
LOCUS       DATDPQ010000256         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_380673, whole genome shotgun sequence.
ACCESSION   DATDPQ010000256 DATDPQ010000000
VERSION     DATDPQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380673"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VI520_05745"
     CDS             <1..280
                     /locus_tag="VI520_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HLF37417.1"
                     /translation="WRPLGLVTVYRTVSLLAELGYLRRVHLEDRCVGYVRSELVHGHH
                     LLCRTCHQAVEFPGTEDLSPLVERISQDTGFQVEHHLLELVGLCPNCQ"
     gene            262..1218
                     /locus_tag="VI520_05750"
     CDS             262..1218
                     /locus_tag="VI520_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HLF37418.1"
                     /translation="MPELPMSRILALLFGWALLAVTAGCQASTAGGSSLEDSGARPRV
                     VATTSLIGDALSRVAGDRIELRVLLPARVDPHSFQSSPQDAAAVAEAQLVFINGFGLE
                     EALLGTIESVADPALLVSISEGIEPREIAAAEQEPREAGTADPHVWLDPTNVMVWVEN
                     ARAALTRADPGGADQYAANAAAYRAELEELDAWIRSEVERIPSENRELVTDHDNLGYF
                     AARYGFTLVGAVIPAYSTLAQPSAQEIAELEQTIARYDVPALFVGVAVNPVLSQRIAQ
                     DMGVDVVALYTDSLSGPGGPAATYLEMMRYTVGAMVEALTIP"
     gene            1215..2006
                     /locus_tag="VI520_05755"
     CDS             1215..2006
                     /locus_tag="VI520_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HLF37419.1"
                     /translation="MSLPQQHDSQAPPIRADRLSVRYDGNLGLEQVSFTVAAGERVAV
                     VGPNGAGKTTLFKVLAGILQPAAGSVEIHGHAPGRHVCVGYVPQRATINLDFPVSVAE
                     VVMMGRIREIGLLRWPSRADWSRVDSALTRVGMQFYRARPIGSLSSGQQQRIFLAQTV
                     AQEADIVLLDEPLTGLDLPSQEVIFEILDDLRHRPVTVLVATHDLSLASSRFDKVLLL
                     NRRLIAYGEPQATITPQNLFEAYGGHLHVFADGKLLVDTHHEGRQ"
     gene            2014..>2310
                     /locus_tag="VI520_05760"
     CDS             2014..>2310
                     /locus_tag="VI520_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HLF37420.1"
                     /translation="MEPILAWLVEPLTYPFMLRGLWAALMIGLVVPVLGCYVVLRGMA
                     FYGDALAHIILPGVVVAFLLGWPLAVGALIVGVLAALTIGGISSSTTIREDSAIG"
BASE COUNT          392 a          731 c          741 g          446 t
ORIGIN      
        1 ctggcgcccg ctcggactgg tgacggtata ccgaacagtc tcccttttgg ccgagctcgg
       61 ctatctccga cgggtgcacc tggaagatcg ctgcgtcggg tatgttcgct ccgagctcgt
      121 gcacggacat catctgctct gtcgcacctg ccatcaggcg gtcgagttcc ccggcacgga
      181 ggacttgagc ccgctggtgg aacggatctc gcaggacacg ggctttcagg ttgaacacca
      241 tctgctggaa ttggtcggcc tgtgcccgaa ctgccaatga gccggatact cgccctcctg
      301 ttcggttggg ccttgcttgc tgtgaccgcc ggctgccagg cctcgacagc cggcggcagc
      361 tcgcttgaag actccggcgc gagaccgagg gtcgtggcca ccacttccct gatcggggac
      421 gcgttatccc gagttgccgg tgatcggatc gagcttcggg tcctgctgcc ggcgagggtc
      481 gatccacact ccttccaaag tagcccccaa gatgcagcgg cggttgccga agcccagctc
      541 gtgtttatca acggatttgg actcgaagag gcactgctgg ggactattga atccgttgcc
      601 gacccggccc tgctcgtttc catctcggaa ggcatcgaac cccgtgagat cgcggcggcc
      661 gagcaagagc ctcgcgaggc cggcactgcc gatccgcatg tctggcttga cccgacgaac
      721 gtaatggtct gggtcgagaa tgcccgcgcc gcactcacaa gagcggatcc gggcggcgcg
      781 gatcaatacg ccgccaatgc ggccgcttac cgggccgagt tggaggaact ggatgcgtgg
      841 atccgcagtg aggtcgaacg aataccatcg gagaaccggg agctggtgac tgaccacgac
      901 aacctgggtt acttcgctgc ccgctatggg ttcacgctgg tgggagccgt gatcccggcc
      961 tacagcaccc tggcccagcc atcggcccaa gagatcgccg aactggagca gacgatcgcc
     1021 cgctacgatg tgccagccct cttcgttggg gttgcggtta acccggttct gtcacagcgg
     1081 attgcacaag acatgggcgt ggacgtggtt gcgctctaca ccgactcgtt gagcggcccc
     1141 ggcggcccgg ctgccaccta tttggagatg atgcgataca cggtcggggc gatggtcgag
     1201 gcgctcacta tcccatgagt ctgcctcaac agcatgattc ccaggcaccc ccgatccggg
     1261 ccgaccggct gtcggtgcgg tacgacggta acctggggct ggagcaggtc agcttcacag
     1321 tggcggccgg cgaacgggta gccgtggtcg ggcccaacgg agccggaaag accaccttgt
     1381 ttaaggtcct ggccggcatc ctgcagcccg ccgccggctc ggtcgaaatc catggccacg
     1441 ccccgggaag gcacgtgtgt gttggatacg ttccccagcg agcgacgatc aatctggatt
     1501 tccccgtctc ggttgccgaa gtcgtcatga tgggccggat ccgcgagatc ggcttgctgc
     1561 gctggccgag ccgcgccgac tggtcgaggg tcgactcggc cctgactcgg gtcgggatgc
     1621 agttctaccg cgcccgaccg atcggaagcc tctcctccgg acagcaacag cgcatcttcc
     1681 tggctcagac cgtcgcccag gaagccgaca ttgtgctgtt ggacgaaccc ttgaccgggc
     1741 tcgacctgcc tagccaggag gtcatcttcg agatcctgga tgatttgcgc caccggccgg
     1801 tcaccgtcct ggtggccact cacgatctca gcctggccag cagtcgattc gacaaagtct
     1861 tgctgctcaa ccgccgcttg atcgcttacg gggagccgca ggccacgatc actccacaga
     1921 acctgttcga ggcctatggc gggcatctgc acgtgttcgc cgatgggaag ctgctcgtgg
     1981 atacccacca cgaaggccgc cagtgaagta gccatggagc caatcctcgc ctggctggtc
     2041 gaaccgctaa cctatccctt catgctgcgc gggctgtggg cggcattgat gatcggtctg
     2101 gtggtaccgg tgctgggctg ctatgtcgta ctcagaggga tggcgttcta tggggacgcg
     2161 ctggcccaca ttattctccc cggagtcgtt gtcgcctttc tgcttggctg gccgctggcg
     2221 gtcggcgcac tgatcgtggg tgtgctggcg gcactcacca tcggaggcat cagcagctcg
     2281 accacgattc gggaggacag cgcaattgga
//
LOCUS       DATDPQ010000257         5346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_906962, whole genome shotgun sequence.
ACCESSION   DATDPQ010000257 DATDPQ010000000
VERSION     DATDPQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906962"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VI520_05765"
     CDS             complement(<1..362)
                     /locus_tag="VI520_05765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF37421.1"
                     /translation="MAAEVAICGAGIAGISTAYHLAVARGLTDVVLIDERPPLSLTSD
                     KSTECYRNWWPGPGDAMVAMMNRSIDILESLARDHGDPFNLTRRGYLFASAEPTRIEE
                     FRAQGREAAALGAGPLRE"
     gene            complement(352..957)
                     /gene="folK"
                     /locus_tag="VI520_05770"
     CDS             complement(352..957)
                     /gene="folK"
                     /locus_tag="VI520_05770"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLF37422.1"
                     /translation="MRNPARCGSPAPWGSRSSGRPAALGELAFVSIGSNLDPEHNLPR
                     AIERLSQLGRLRRISGVYQNQAIGGRPQPDYLNAAVLLDVDRPPDALRAELRRIEAEL
                     GRVRTSDKYAPRTIDLDLSLFGDQIYRGPGFQIPDPQITRLAHLAIPLADLAPEYRHP
                     ELVESLAEIADRLRAGSKLVLRTDLSEEINDLLAAPGDNGR"
     gene            complement(881..1261)
                     /gene="folB"
                     /locus_tag="VI520_05775"
     CDS             complement(881..1261)
                     /gene="folB"
                     /locus_tag="VI520_05775"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HLF37423.1"
                     /translation="MTSESPADLIVIKDLLLRGMIGVNDWEREVEQDILVNITLTADL
                     SRAGQTDDLADTVNYRTVAKKIIAHVEGTQRFTLEALAADIAAICLAEPGVRRARVRV
                     EKPGALRFARSVGVEIEREAGSAG"
     gene            complement(1263..2000)
                     /locus_tag="VI520_05780"
     CDS             complement(1263..2000)
                     /locus_tag="VI520_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF37424.1"
                     /translation="MQSEFDLSGHTALVTGAGRRLGRAFAEAIARQGGDLVVHYRHSQ
                     SGAEQAVQVAEQFGCRAVAIQADLTDPEQAAGLIRHSLELVPQVDLLVNSAAIFEPVG
                     ALEASLEDWQRHFSVNLTSPFLISQAFARARNGAPGVIVNLLDWRALLPAADHFPYSI
                     SKAALAAMTRGLAVALAPSVRVNGLALGAILPAGDAASGDPIAGVPAGRWGTVQEAVE
                     GLLFLLAGPKSMTGEILQLDGGRHLTG"
     gene            2090..2749
                     /gene="folE"
                     /locus_tag="VI520_05785"
     CDS             2090..2749
                     /gene="folE"
                     /locus_tag="VI520_05785"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HLF37425.1"
                     /translation="MDIEEHLNTIEHKLEIEAAAELLSGVGVARPEVQEAVRNLLIAV
                     GEDPEREGLQATPKRVARAYDELLAGYRVDPHQMINDALFNEAYDEMVIVRDIEFYSL
                     CEHHMLPFIGKAHVAYLPNGKVLGLSKIPRVVDLFARRLQVQERMTRQIADFLDTALH
                     PHGVGVVVEGLHLCAMMRGVKKANARMITSALCGTFSESPLTRQEFLEHLSRAGSHTS
                     L"
     gene            2785..3087
                     /locus_tag="VI520_05790"
     CDS             2785..3087
                     /locus_tag="VI520_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37426.1"
                     /translation="MAYARAAREHGKDGRARVSARRAAGWAVNAFLSRSAEPPYRGAF
                     DLLGWLERNGPAELRPPAARLRQQVTKDHRLPHDEDPLEDAELIVHTLLGLMNTTQ"
     gene            3084..3767
                     /locus_tag="VI520_05795"
     CDS             3084..3767
                     /locus_tag="VI520_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLF37427.1"
                     /translation="MMGPIDVGSDQAPLAFRIRAAIDLEIPPTVMLHGLGGDEQAIWE
                     LAPALPPGGLIVAARAPYLQAAGGYGWLPQIGAWPPKLEEFAESVRLLETLLRYLDQN
                     HGLDRGRLVLLGFSQGGAMAFAAAMIAEAGLSIPPAAIAVGAGHLPLGDLSPLRGLPV
                     FWGHGTRDELIPVSVARNDVERLRAAGAQVQYCEADVTHKLGLACLRELKQWIPVRSA
                     DPHKDLLSR"
     gene            3764..>5346
                     /gene="tkt"
                     /locus_tag="VI520_05800"
     CDS             3764..>5346
                     /gene="tkt"
                     /locus_tag="VI520_05800"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLF37428.1"
                     /translation="MNDSDLATRSINTIRFLAADAVQKANSGHPGLPMGAASLAYVLW
                     TRHLRFDPADPKWPNRDRFVLSAGHGSMLLYAMLHLTGYDLPMEELQRFRQWGSLTPG
                     HPEHGDTPGVETTSGPLGQGFANAVGMGVAAERMAAYYNREGYPIVDHFIYAIVSDGD
                     LMEGIGSEAASLAGHLRLGRLIYLYDDNRISIDGSTDLAFTEDRAARFQAYGWHVSRV
                     DDFLDLEAIHAALLAAEADPRPSLIVCRTHIGYGLPTRQDTAEAHGEPPGEQELSGAK
                     DKLGWPQQPRFLVPQDVRSHLQSSAGRGRAARSGWLAMLSEYRREQPDLAKTWDRTQT
                     GQLPGGLRDSLPEFAPDEKGLATRAASGKTLNALAMGLPELIGGSADLSGSNLTKISG
                     EAPFSASHRIGRYVHFGVREHAMGGMLSGMALYGGLVPYGGTFLIFSDYMRPSLRLAA
                     MMKLRVIYVFTHDSIGLGEDGPTHQPIEHLAALRAIPNLTVIRPADANETAFAWWAAL
                     QRTGGPTALALTRQKLPNF"
BASE COUNT          961 a         1677 c         1745 g          963 t
ORIGIN      
        1 tgttctcgca gcgggccggc ccctagggca gcggcctccc ttccctgggc tcgaaactct
       61 tcgatccggg tcggctcggc cgaggcgaag aggtagccgc ggcgggtcag gttgaacggg
      121 tctccgtggt cgcgggcgag cgactcgagg atatcgatgc tgcgattcat catcgccacc
      181 atcgcgtctc ccggcccggg ccaccaattc cgataacact ccgtggactt gtcgctggtc
      241 aacgagaggg gcggtcgctc atcgatcagc accacgtctg tgagcccgcg ggcgaccgcc
      301 aggtggtagg ccgttgagat cccggcgatc cctgccccgc agatcgcaac ttcagcggcc
      361 attgtctcca ggcgccgcga gcagatcgtt gatctcctcg ctcagatcgg tacggagcac
      421 cagcttcgag ccagctcgca gtcggtccgc aatctctgcc aagctctcaa ccagctccgg
      481 gtggcggtac tcaggcgcca gatcagcgag cgggatggct aggtgggcga gccgggtaat
      541 ctgcggatca ggaatctgga aaccaggacc gcggtagatc tgatcgccaa agagagataa
      601 gtcgaggtcg atggtccgcg gggcgtactt gtcgctcgtc cggacccgcc ccaattcggc
      661 ttcgatcctg cgcagctcag cccgcagcgc gtcgggaggg cggtccacgt caagcagcac
      721 ggctgcgttc aagtagtccg gctgcggtcg acctccgatc gcctgattct gataaacccc
      781 cgatattctc cgcaagcggc cgagctggct caatcgttcg atggcccgag gcaaattgtg
      841 ttcaggatcc aggttcgagc cgatgctgac gaaggctagt tcacccagcg ctgccggcct
      901 cccgctcgat ctcgaccccc acggagcggg cgaaccgcag cgcgccgggt ttctcaaccc
      961 ggactcgagc gcgccgcact ccgggttcgg cgaggcagat tgctgcgata tcggccgcca
     1021 gggcttccag cgtgaatcgc tgagtgcctt ccacatgggc gatgatcttc ttggccaccg
     1081 tccggtagtt gactgtgtct gcgagatcgt cagtctggcc ggctcggctg agatctgcgg
     1141 tgagggtaat gttgaccagt atgtcttgtt cgacttcccg ctcccagtcg ttgaccccaa
     1201 tcatcccgcg caggagcagg tctttgatga cgatcaggtc ggctggcgat tcggaggtca
     1261 tctcatccgg tcagatgccg gcctccgtca agctgcagga tctcacctgt catcgatttc
     1321 ggtccggcca gcaagaacag caacccctcc acggcctcct ggacggtgcc ccagcgtccg
     1381 gcaggcactc ccgcgattgg atcgccgctt gccgcgtcgc cggccgggag aatcgcgccg
     1441 agggccagcc cgttgacccg taccgagggt gccagggcca cggccagacc acgggtcatg
     1501 gcggcgaggg cggccttact gatcgaatat gggaaatgat cggcggccgg caggagcgcc
     1561 cgccagtcga gcaggttgac aatgaccccg ggtgccccgt tgcgggcccg ggcgaaggcc
     1621 tgcgagatca ggaacggcga agtgaggttg acgctgaaat ggcgctgcca gtcttccaga
     1681 gaggcctcga gcgcgccgac cggctcgaag atcgccgcgc tgttgaccag caggtcgact
     1741 tgaggcacca gttccagaga atgccgaatc agcccggcgg cctgctctgg atcggtcaga
     1801 tcggcttgga ttgccacggc ccggcacccg aactgctctg cgacctgcac ggcctgctcc
     1861 gcaccggatt gggaatgacg ataatggacg accaggtcgc ctccctggcg ggcaattgct
     1921 tcggcgaagg cgcggccgag tcgtcggccc gccccggtca ccagcgcggt atgcccgctc
     1981 agatcgaact ccgattgcat tcgggtattg tacccgtgcg atcactcgga tcagatatgg
     2041 taagctcttg ccgcctgtgc tagtcaaacg attcgtacag aaggaaacca tggatatcga
     2101 ggaacatctc aacacaatcg agcacaaact tgaaatcgag gccgcggccg agttgttgag
     2161 cggcgtcggg gtcgcccggc cggaagtcca ggaggcggtt cggaacctct tgattgcagt
     2221 gggggaagac ccggaacggg agggtctgca agcgacgccg aagcgggtgg cccgggccta
     2281 cgatgagctg ctggccggct accgagtcga tccgcaccag atgatcaacg acgccctctt
     2341 caacgaggct tacgacgaga tggtgatcgt gcgcgacatc gaattctaca gcttgtgtga
     2401 gcatcatatg ctgccgttca tcggaaaggc acacgttgcc tacttgccga atggcaaggt
     2461 tctcggtctc tccaagatcc ctcgggttgt cgatctcttt gcccgccgac tgcaggtcca
     2521 ggaacgaatg acccgccaga ttgccgattt cttggacacg gctctccatc cgcacggcgt
     2581 cggggtcgta gttgaaggtc tgcacttgtg tgccatgatg cgcggggtga agaaagccaa
     2641 tgcacggatg atcaccagcg cgctctgtgg gacgttctca gagagtccgc tcacccggca
     2701 agagtttctc gagcatctct caagagctgg cagccacacg agtctgtgag ggcggagcag
     2761 tctaggtcca ggatccaggc cgaattggcc tatgcccggg cagctcgcga acacggaaaa
     2821 gacgggcggg cccgggtctc ggcccgccgg gcggcgggtt gggcagtaaa cgctttcctc
     2881 agccgcagcg cggaacctcc gtaccgcggg gccttcgacc tcttgggctg gttggaacgg
     2941 aatggacccg cggagctgcg cccgccggcc gcccgcctga ggcagcaagt gactaaagac
     3001 caccgcctgc cccacgacga agatcccttg gaagacgctg aactgattgt ccataccctg
     3061 ctaggcctga tgaatacgac ccaatgatgg gtcccattga cgtcggctcc gaccaggcgc
     3121 cgctcgcatt cagaattcgg gccgcgatcg atctcgagat tccgccgacc gtgatgctgc
     3181 atggtttggg tggagacgag caggccattt gggaactggc gccggcactt ccgcccgggg
     3241 gcctgattgt cgctgctcgg gctccctatc tccaggccgc cggcggatat ggctggttgc
     3301 cgcagatcgg ggcgtggccg cctaagctgg aggaattcgc agagtcggtc aggcttctgg
     3361 aaaccctgct ccgctacttg gaccaaaacc acggattgga tcgggggagg ctggttctgc
     3421 tcggcttcag ccagggcggg gcgatggcct ttgctgcggc gatgattgcc gaggctggat
     3481 tatcgattcc gccagcagca attgcggtgg gggccggtca tctcccgctg ggcgacctaa
     3541 gcccgctccg gggattgccg gtattctggg gccacggcac ccgggatgag ctgatcccgg
     3601 tgtcggtggc ccgaaatgat gtcgagcgcc tgcgagcggc cggtgctcag gtgcaatact
     3661 gcgaggccga cgtcacccac aaactagggc tggcctgcct gcgagagctc aagcagtgga
     3721 tcccagtccg atccgccgac ccgcacaagg acctgctcag ccgatgaatg actcggacct
     3781 cgcgacccgg tccatcaata cgatccggtt cctggcagcc gatgcggtgc agaaagccaa
     3841 ctcgggccat cccggtctcc ccatgggggc agccagcctg gcctacgtgc tctggactcg
     3901 ccacctgcgg ttcgacccgg cggacccgaa gtggccgaac cgggatcggt tcgtgctctc
     3961 ggccgggcat ggctccatgc tgctgtacgc gatgctgcac ctcaccggct acgaccttcc
     4021 aatggaggaa ctccaacgct tcaggcaatg gggcagcctg acccccggcc atccggagca
     4081 cggcgatacg ccgggggtgg agaccaccag cggacccctg gggcaggggt tcgccaacgc
     4141 ggtcgggatg ggggtggccg cagaacggat ggcggcctac tacaaccggg aaggctatcc
     4201 gattgtcgat cacttcatct atgcgatcgt ctcggacgga gacttaatgg aagggatcgg
     4261 ctcggaggcc gcctcgttgg cgggacacct ccgcctgggg cgattgatct acctgtatga
     4321 cgacaaccga atctcgatcg atggcagcac cgatctggca ttcacagaag atcgggcggc
     4381 gcgatttcag gcctatggat ggcatgtgag ccgcgtcgat gatttcttgg acctcgaagc
     4441 gatccacgcc gcgctgctgg ccgcggaggc cgacccacgc ccttcgctga ttgtctgccg
     4501 gacccacatt ggctacgggt tgccgaccag gcaggacacc gcggaggctc atggagagcc
     4561 gccgggagaa caggaactaa gcggggctaa ggacaaactg ggttggccgc agcagccgcg
     4621 attcctggtc ccccaggacg tccggtccca tctgcagtcc tcagccggtc gtggccgcgc
     4681 tgcgaggtcg ggctggctcg cgatgctgtc cgaatatcgg cgggaacagc ctgatctggc
     4741 caagacctgg gatcgaactc agaccggcca gctgccgggc ggcctgcgag atagcctccc
     4801 agagttcgcg ccggacgaga aaggcctggc gacccgggcg gcttccggca agaccctcaa
     4861 tgccctggct atggggcttc ccgagttgat cggcggttcg gctgacctca gtggatcgaa
     4921 cctgaccaag atcagcggcg aggctccctt ttcggcttcg caccggatcg gacggtacgt
     4981 tcactttgga gtgcgtgagc acgcaatggg ggggatgctg agcgggatgg ctttatacgg
     5041 cggccttgtt ccgtacggcg gcacgttcct gatcttctcg gactacatgc ggccgagtct
     5101 gcggctggcg gcgatgatga aattgcgggt gatctacgtg ttcacccacg actcgattgg
     5161 gttgggcgaa gacggtccga cccatcagcc gatcgagcac ctggccgcac tgcgggccat
     5221 tccaaatctg accgtgatcc gtccggcgga tgctaatgag accgccttcg catggtgggc
     5281 ggcgctgcaa cggaccggag ggccgaccgc cctcgccctt acccgacaga agctgccgaa
     5341 cttcga
//
LOCUS       DATDPQ010000258         5266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2477138, whole genome shotgun sequence.
ACCESSION   DATDPQ010000258 DATDPQ010000000
VERSION     DATDPQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477138"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1050)
                     /locus_tag="VI520_05805"
     CDS             complement(163..1050)
                     /locus_tag="VI520_05805"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLF37429.1"
                     /translation="MISLNGIFPPIPTPFDSDESIHFNQLKSNLTRWNTWPLDGYVVA
                     GSTGESVSLTSDERVELVRAVRSAIPSEKLLIAGAGLESTRQTVELVRRMADSGADAV
                     IVVTPSYFAPSLGRRGLIAHYLSIAEASSLPLILYSVPIYTHLDLPIEVVRELSTHPK
                     VVGIKESGGSLAKITSLVHQTPKEFQVLVGSAGLLLGALVVGAVGCIPALGNVAATQI
                     RELMTAFQRGDLTRAREVQANLVEPNEILTVRFGVAGVKAALDLVGLYGGPPRSPLQP
                     LGEPELEVVSDALSRAGML"
     gene            complement(1082..1714)
                     /locus_tag="VI520_05810"
     CDS             complement(1082..1714)
                     /locus_tag="VI520_05810"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLF37430.1"
                     /translation="MRIYDVSVGISPEIPVWPGDAPVRLERTQSMDDGAHANVSRFEA
                     GVHVGTHVDAPLHFIRGGRSVDSIPLKSLIGRAYVVELKRAEVLNASTLEGAGIPPRT
                     RRLLFKTRNSEFWTKGERRFRRDFVAVDPSGAEWMVRKGVRLVGVDYLSVAPFGDSVP
                     THRILLEAGVVVVEGLNLAAVPKGRYTIYCLPLKLIGSDGAPARVILVGV"
     gene            1787..3163
                     /locus_tag="VI520_05815"
     CDS             1787..3163
                     /locus_tag="VI520_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLF37431.1"
                     /translation="MLTNWQAIDRAIDNKLESSLEELSRLCAQPSVSAQGVGIQECAE
                     LVAEMLQARGFDTKVLATDGHPVVFGERSGRGDRTLLLYNHYDVQPAEPLELWTTPPF
                     EPSRRNGQLFARGVSDDKGHIQCRLAALDAFLEVEGELPCSVKFVIEGEEEVGSVHLP
                     EFVARHSKLLAADACLWEFGQLNHEGTPVQDAGMRGICYVELSVETASRDSHSGTSGS
                     IFPNAAWRLVWALRTLKGSDEMIRLPGFYDRVVPPTERDLSLLEQIPDPSDHFREFYG
                     IKRYLKGIEGGLDLLREQVFVPTCTICGLTAGYQGPGSKTVLPARASAKVDFRLVPDQ
                     RPSEVLEQLRSHLDAEGFPDVEIRELGSEPPARTDPDDPFLQLVVNSAVEVYGVPQLI
                     SPLVGGSGPNHAFIHGLGVPVATAGLGYPGSNVHAPNENIVIDYFVRGARHTARVLGY
                     FGAGVTLP"
     gene            3199..3273
                     /locus_tag="VI520_05820"
     tRNA            3199..3273
                     /locus_tag="VI520_05820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3234..3236,aa:Pro,seq:ggg)
     gene            3353..4081
                     /locus_tag="VI520_05825"
     CDS             3353..4081
                     /locus_tag="VI520_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLF37432.1"
                     /translation="MRVQLFATCLVDSLFPEVGEAVVEVLTRAGVETWFPPDQTCCGQ
                     PALNAGSRAEARTLALHTLDALAADDVVVVPSGSCSQMIRHHYPELLADQPEELERAR
                     RLADRTFEFSEFLVDQLLVADFEASLPLVIGYHPSCHLLRGLGVDRQPLRLLEGVAGA
                     SIERLSPECCGFGGLFSVEMPEISREMLERTLERIRSSGVELVTGCDVSCLMQIEGGL
                     RRAGAPVRCSHLAQLLAGQEAGLK"
     gene            4078..>5266
                     /locus_tag="VI520_05830"
     CDS             4078..>5266
                     /locus_tag="VI520_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HLF37433.1"
                     /translation="MTLAFRQRARRAIADPQLQRLLDQNADARQRARMEALQELPEAS
                     DLRQRARSIRQQVVFGLEAYLEQFGSRLADNGVQLRRAADAAEANRLILEIVRSHKAN
                     LVAKSKSMLTEELGLNAALEAAGVRAIETDLGEYVVQLRGEKPAHIMTPALHLSTEQV
                     AETFRTQLGAGLSDDAEQINAEVRSRLREVFLTAPVGITGVNFGVAETGTLCLVTNEG
                     NGRMVATLPPVHIAVMGIERLVPTLDDLAVMLRLLPRFATGQSISSYVSLIHGPRRPG
                     DPEGPAERYLVLVDNGRSRVAETAAAEALLCIRCGSCLNACPIFREAGGHAYDSVYSG
                     PIGSVLSPALFGLEKYGHLAQASTLCGACMEACPVGIDIPGLLLDVRAQSDPKPLSRA
                     IRLF"
BASE COUNT          942 a         1671 c         1702 g          951 t
ORIGIN      
        1 tgcggcgagc tgctggccgc ccacatcggg ggtggcgagc tgcctggcta cgctcgctgg
       61 ttccggctgg agcggtacgc cgatcccgag taccaggaca tgcttgcgaa ctggggccgc
      121 agcggtcagc tttgagcagc tgtcgccacg cggattgatc ggctagagca ttccggcccg
      181 actcaacgca tcgctcacga cctccagctc gggttcacca agcggctgca gcggcgagcg
      241 cggcggtccg ccatagaggc caaccaggtc aagggcggcc ttgaccccgg caaccccgaa
      301 gcggacagta agaatctcgt tgggctcaac caggttcgcc tgaacttccc gcgcccgggt
      361 cagatctcct cgctggaacg ccgtcattaa ttcacggatt tgggtcgccg ccacatttcc
      421 tagcgccggg atgcagccga ccgccccgac caccagtgcg ccgagcagga gtccggcaga
      481 cccgactagc acttggaact ccttcggcgt ctgatggacg agcgaggtga tcttggccag
      541 gctgccgccg ctctccttga ttccgactac cttcgggtgg gtcgaaagct cccggacgac
      601 ctcgatgggc agatccaggt gggtatagat cgggacactg tagaggatga gcggcaggct
      661 cgaggcctcg gcgatggaca ggtagtgggc gatcagtccg cgtcggccga ggctgggggc
      721 gaagtagctc ggagtgacga cgatcactgc gtccgcaccg gaatcggcca tccgcctaac
      781 caattcgacg gtttggcgag tggactccag gcccgccccc gcgatcagca atttttcgct
      841 cgggatggca gaccgcaccg cccgaaccag ctcaactcgc tcatcggaag tcagcgagac
      901 cgactctccg gtcgagccgg ccaccacata gccgtcaagc ggccaagtgt tccagcgggt
      961 cagattggat ttcagctgat tgaaatggat tgattcgtca gagtcgaagg gggtcgggat
     1021 cggagggaag atgccgttca gcgaaatcaa gggtcaagtg gggcttgggc ggtcgagtag
     1081 ctcagacccc gaccaggatc acccgggccg gtgcgccatc gctgccgatc agcttgagcg
     1141 gcaggcagta gatcgtgtaa cgacccttag gcactgcggc caggttcaat ccctcaacaa
     1201 ccaccacgcc ggcttccaag aggatccggt gggtaggcac actgtcgcca aacggtgcca
     1261 ctgaaagata gtcgaccccc acgagccgga cacccttgcg gaccatccat tcggccccgc
     1321 tgggatcgac cgccacgaag tcacgccgaa acctgcgttc acccttggtc cagaactccg
     1381 agttgcgggt cttgaacagc agccgccgag tgcggggcgg gattccggcc ccttcgaggg
     1441 tggaggcatt caagacttcc gcccgcttca actcaacgac gtaggcccgc ccaatcagtg
     1501 acttaagcgg aattgaatcg acgctgcgtc cgccacgaat gaagtgcagc ggagcatcga
     1561 catgcgtgcc gacatgtacg ccggcctcga agcgcgagac attggcgtgg gctccatcgt
     1621 ccatcgactg ggtccgctcg agccgaaccg gcgcgtcgcc cggccagacc gggatctctg
     1681 gcgaaatccc aaccgagacg tcgtaaattc tcatgaaagg cggcggatta tagattcgat
     1741 cccaacagaa tgcgaacttg gtctgtcccg aggctataat cggcccatgc tcaccaactg
     1801 gcaggcaatc gaccgcgcaa tcgacaacaa gcttgagagc tcgctggaag aactgagcag
     1861 attgtgtgcc caacccagtg tgtcggctca gggggtgggg atacaggagt gcgccgagct
     1921 ggtggccgaa atgcttcagg cccgaggctt cgacaccaaa gtcctggcga ccgacgggca
     1981 cccggtcgtg tttggcgagc ggtccggccg gggcgatcga actttgctgc tgtacaacca
     2041 ctacgatgta cagcccgccg agccgctcga actgtggacg acgcccccct tcgaaccgag
     2101 ccggcgcaac ggccaattgt ttgcccgagg cgtcagcgac gacaagggcc atatccagtg
     2161 taggcttgcc gccctggatg cgtttctgga ggtcgaaggc gagctgcctt gttcggtcaa
     2221 gttcgtcatc gaaggcgagg aggaggtcgg cagcgtccat ctgcctgagt tcgtggcccg
     2281 ccactccaag ctgttggcag cggacgcctg cctgtgggaa tttgggcagc tcaaccacga
     2341 gggcaccccg gtccaagatg ccggcatgcg cggcatctgc tacgtcgagc tgagcgtgga
     2401 aaccgcgtcc cgcgactcgc attcgggcac cagcggctcg atcttcccca acgcagcctg
     2461 gcgcctggtc tgggcgcttc gcaccctcaa aggttcggat gagatgatcc ggcttcccgg
     2521 cttttacgat cgggtagtgc cgcccaccga acgcgatctg tcgctgttgg agcagatccc
     2581 cgacccgagc gatcattttc gggagttcta tggaatcaag cgatacctta agggaatcga
     2641 gggcggtctg gacctgctgc gggagcaggt cttcgtgccg acctgcacca tttgtggatt
     2701 gacggccggc tatcaaggcc ccggcagcaa gactgtgctc ccggcccggg ccagcgcgaa
     2761 ggtcgacttc cgcctggtcc cggatcagcg accgagcgaa gtcttggagc agctgcggtc
     2821 tcacctggac gccgagggct ttccggacgt cgagattcgc gagttgggca gcgagccgcc
     2881 cgcccggacg gatcccgacg atccgttctt gcagctcgtt gtcaactcgg cagtggaggt
     2941 ctatggtgtt ccgcaattga tcagcccgtt ggtgggaggc tccggcccca accacgcctt
     3001 tatccatggg ctcggggtcc cggtggcgac tgccggtctc ggctaccctg gttccaacgt
     3061 tcatgccccg aacgagaata tcgtcattga ttacttcgtc cggggcgcac gccacacggc
     3121 tagagtcctc gggtatttcg gcgcgggggt gacccttccg tagccgagcg ggccaatcta
     3181 taatccaccc agaaccctcg gggcgtagcg cagcccggtt agcgcacctg catggggtgc
     3241 agggggtcgg cggttcgaat ccgcccgccc cgactacgga ccgtcgcacc ttcgcggcgg
     3301 tcccgccgtt tcacacccgg ttcaaccgaa cgaggccagt gcaacaatac cgatgagggt
     3361 ccagctattt gccacctgtc tggtggatag cttgtttccc gaggtcgggg aagcggtcgt
     3421 ggaggtgctg acccgggccg gggtggagac ctggtttccg ccggaccaaa cctgctgcgg
     3481 gcaaccggcc ctcaacgccg gttcgcgggc cgaagcgcgt acactggcgc ttcacaccct
     3541 ggacgccctc gccgcggacg acgttgtggt ggtgccgtcc ggatcctgct cccagatgat
     3601 ccgccatcac tatccggaat tgctggcgga tcaacccgaa gagttggagc gagcccggcg
     3661 gctggccgac cgaacattcg agttcagtga gtttctggtc gatcagctgc tagtggcaga
     3721 tttcgaggcc tcccttccgc ttgtaattgg atatcacccc tcctgccacc tgctgcgggg
     3781 attgggtgtg gaccgccagc cgcttcgact gctcgaaggg gtcgccggag cttcaatcga
     3841 gcgactcagc ccggagtgct gcgggtttgg cgggctattc tcggttgaga tgcctgagat
     3901 ttcccgagaa atgctcgagc gcaccctgga gcggattcgg agctccggcg tcgagcttgt
     3961 cacgggatgc gatgtgagtt gtctgatgca gatcgagggt ggcctgcggc gggccggcgc
     4021 tccggtgcgc tgttcgcatc tggcgcagct cttggccggc caggaggcgg ggctgaagtg
     4081 accttggcct ttcgccagcg ggcccgccgc gcgatcgcgg atccccagtt gcaacgactg
     4141 ctcgatcaga acgccgacgc ccgccagcgg gctcggatgg aggcactgca ggaactcccc
     4201 gaggcgagcg atctgcggca gcgagcccgt tcgattcgcc agcaggtggt gttcgggctg
     4261 gaggcctacc tggagcagtt tgggtcccgc ttggcagaca atggcgtcca gctccggcgg
     4321 gcggccgacg cggccgaagc caaccggctc atcctggaga tcgtccgaag ccacaaggcc
     4381 aatctggtgg ccaagtccaa gtcgatgctt accgaggagc tgggcctgaa cgccgccctc
     4441 gaggccgccg gagtccgagc catagagacc gatctgggtg agtatgtcgt tcagcttaga
     4501 ggcgagaagc cggcccatat catgacccct gcgctgcacc tgagcactga gcaggtggcc
     4561 gaaactttcc gcacccagct tggcgcaggc ctgagcgacg acgcggagca gatcaacgcc
     4621 gaagtccgca gccgattgcg ggaagtattc ttgaccgccc ccgtcgggat cacgggcgtg
     4681 aacttcggag tagctgagac cggtacgctg tgtttggtca ccaacgaagg aaacggccgg
     4741 atggtcgcga ctctgccgcc cgtgcacatc gcagtgatgg ggatcgaacg actggtcccc
     4801 accctggatg acctggcggt gatgctgcgg ctgttgcccc gcttcgcaac cggtcagtcg
     4861 atcagcagtt acgtgtcgct gatccacggt ccccgccgac ctggcgatcc ggaaggtccc
     4921 gctgaacgat acctggtcct ggtagacaac ggccgctcaa gagtggcaga gactgcggcg
     4981 gccgaggccc tcctgtgcat ccgctgcggc tcctgcctca acgcctgtcc gatctttcgg
     5041 gaggccggtg gccatgccta tgacagcgtc tactccgggc cgatcgggtc cgtcttgtcg
     5101 cctgctctgt tcggcctcga gaagtacggc catctcgccc aggcctcgac cctctgcggg
     5161 gcttgcatgg aggcctgccc ggtcgggatc gatatcccgg gcctcctgct ggatgttcgg
     5221 gcccagagtg acccgaagcc actcagccgc gccatccggc tgttca
//
LOCUS       DATDPQ010000259         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_123311, whole genome shotgun sequence.
ACCESSION   DATDPQ010000259 DATDPQ010000000
VERSION     DATDPQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123311"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VI520_05835"
     CDS             complement(<1..366)
                     /locus_tag="VI520_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF37434.1"
                     /translation="MSKPKPVRLLHYLLLGGLGITLMIVSGRAGGAPAFAQAAIDGET
                     IYLERCAFCHGENGDGSGPVARYLNPRPRNFTLGQFRFRTTASGELPLRSDVIAIVKS
                     GVNGTAMPRWEGVLSDAEIE"
     gene            complement(359..1156)
                     /locus_tag="VI520_05840"
     CDS             complement(359..1156)
                     /locus_tag="VI520_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF37435.1"
                     /translation="MTRRLSLFWMVVLTLVGVNLLLRVGVPYLSMLISDRAEPLPVPG
                     ALMVIYQALTAIGVLVYLAADERRLHEFWLPIRTFIRGPEAVRTPFDRALAYARWGVF
                     AALPLLAGWAVYSSSLPATAAPTSLRISHPTIPFEYQSLTNPFRNPDGTVDPAVIEEG
                     RVLFQINCRPCHGTPANGEGPMAYGFRLRPANFSSEDTIATVIEAYAFWRVREGGIGL
                     PNAASPWDSAMPAWAGELTDEQIWKIIAAEYQIAGVEPRRPESGPGE"
     gene            complement(1211..2335)
                     /locus_tag="VI520_05845"
     CDS             complement(1211..2335)
                     /locus_tag="VI520_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF37436.1"
                     /translation="MRLLRVAAGLACLGSFVALLADLPPVLLPWIFWFLAAAGLASYL
                     SPGLRTGLFVLVMLGLFVFMGALVTRISGGEFQVAAAEGISVEAGEAIYWGKGKCSTC
                     HSLGDRGSAVRGPNHADVCAKARDLRVSERQAAGATQIQTATDYLVESLAEPDAYIVE
                     SFSAAMPKVYLPPISLTAEELSAVIVYLQAQGCAPDPAAIELPAAILNAAAAQAETGA
                     PFSLVVVGDPAAGRALFSDSAGPAGCVQCHTVAGQGGSVGPELTEVAGTQTLAYIFES
                     IMNPSAAIAAGGYEPIQLQLTDGSVLSGVIEAEDDASITLVDKEGTQAVVHKSDIQRE
                     RRYPDIPSLMPGNFGELLTVKQVADLIAFLQESAGAVPVE"
     gene            complement(2332..>2683)
                     /locus_tag="VI520_05850"
     CDS             complement(2332..>2683)
                     /locus_tag="VI520_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37437.1"
                     /translation="LGEGTSELAKIEGVREAGDGDPYTAVVISGYALYGKLRGFYSVG
                     VSFIRVDSQSGVANYASEESALGKESVASLSPKQRAAIAEWLNHFDPGAWENSTEPFK
                     LSLTQPALQPEPAG"
BASE COUNT          502 a          893 c          846 g          442 t
ORIGIN      
        1 ttcgatctcc gcgtcgctga ggacaccttc ccaacgcggc attgcggtcc cgttcacccc
       61 actcttcacg atcgcgatca catcgctgcg cagcggcagt tcgcccgaag ccgtggtccg
      121 gaagcggaac tgccccaggg tgaaattccg cggtcggggg ttgagatagc gggcgaccgg
      181 gccactgccg tcgccgttct cgccgtggca gaatgcacag cgttccaggt agatcgtttc
      241 cccatcaatt gcggcttgtg cgaatgccgg cgccccgccc gcccggccgg agacaatcat
      301 caacgtgatc ccgagcccgc cgagcagcag gtagtgcagt aatcgaaccg gcttgggctt
      361 actcaccggg cccggactcc ggtcgccgag gctcgacccc ggcaatctga tactcggcgg
      421 caatgatttt ccagatctgt tcgtcggtca gctcaccggc ccaggctggc atcgccgagt
      481 cccatggact ggccgcgttg ggcaggccga tcccaccctc ccggacccgc cagaaggcgt
      541 aggcctcgat gacagtcgcg atggtgtctt ccgagctgaa attcgccggc cgcagcctga
      601 aaccgtaggc catcgggcct tcgccgttcg ccggcgtccc atggcaaggc cggcagttga
      661 tctgaaacag aacgcgacct tcctcgatga ccgccggatc tacggttcca tccgggttgc
      721 ggaaggggtt cgtcaggctc tgatactcaa atggaatcgt cgggtgcgag attcgcaaac
      781 tggtcggggc cgcggtcgcc ggcaggctgc tggaatacac ggcccagccg gccaagagcg
      841 gcagtgcggc gaagactccc cagcgggcgt aggcgagcgc ccgatcgaac ggggtgcgca
      901 ccgcttccgg cccgcggatg aatgttcgaa tgggcagcca gaactcgtgc agccgccgtt
      961 cgtcagcagc caagtagacc agaaccccga tcgcggtcag agcctggtaa atcaccatga
     1021 gcgcgccggg cacgggtaac ggctcggccc gatcgctgat cagcatggag aggtacggga
     1081 ctcccacgcg cagaagcagg ttgaccccga cgagggtcag cacgaccatc caaaacagcg
     1141 aaagcctgcg ggtcatgctg ggctcagcct cggcatccag gccgccccgc cccgcagcag
     1201 gaatggagaa ttactcaacc gggacggctc ccgcactctc ttgcaagaac gcgatcaagt
     1261 cggcgacttg ctttacagtc agcagctccc cgaagttgcc cggcatcagg ctggggatgt
     1321 ctggatagcg gcgctcgcgc tggatgtctg acttgtgaac cacggcctga gtgccctctt
     1381 tgtccaccag cgtgatactg gcatcgtcct cggcctcaat gaccccggag agcaccgacc
     1441 cgtcagttaa ttggagctgg atcggttcgt aaccaccggc cgcgatcgcg gcgcttgggt
     1501 tcatgattga ctcgaagatg taggcgagag tttgagtccc ggcgacctcg gtcagctctg
     1561 gaccgacgct cccgccctgc ccggcgaccg tgtggcactg cacgcagccg gctggcccgg
     1621 ccgagtcact aaacaacgcc cgccctgcgg ccgggtcacc gactacgacc agggaaaagg
     1681 gagcgcccgt ctcggcctgg gccgccgccg cattgaggat ggcggcgggc aactcaatcg
     1741 cggctggatc gggtgcgcaa ccctgggcct gcaggtatac gatcaccgcg ctcaattcct
     1801 ccgcggtcag gctgatcggc ggcaggtaga ccttcggcat cgcggccgag aagctttcca
     1861 cgatgtaggc atccggctcg gcgagcgatt cgacgagata gtcggtggcg gtctggatct
     1921 gagtggcgcc tgccgcctgc cgctcggaga cccgcaagtc acgagccttg gcgcagacgt
     1981 cggcatggtt cggaccgcgg accgccgagc cacggtcgcc gaggctgtga caggtgctgc
     2041 atttcccctt tccccagtag atcgcttccc cggcctcgac gctgatcccc tcggcggctg
     2101 ccacctggaa ctcgccgcct gaaatccggg tcaccaacgc ccccatgaag acaaaaagcc
     2161 cgagcatgac taacacgaag agaccggtgc ggagcccggg cgagagatag gacgcaagac
     2221 ccgccgccgc caggaaccag aagatccagg gcagcagcac gggcggaaga tcagcgagca
     2281 gcgcgacaaa actgcccagg caggccagcc ctgccgcaac tcggagcaac ctcatccggc
     2341 gggctccggc tgcagcgctg gctgagtgag gctgagcttg aagggctccg tcgaattctc
     2401 ccaggccccc ggatcgaaat ggttcagcca ttcggcgatg gcagcccttt gtttggggga
     2461 gagcgaagcc accgattcct tgccgagggc gctctcttca gaggcgtagt tggcgacccc
     2521 gctttgcgag tccacccgta tgaaggaaac ccctacggag tagaagccgc gcagctttcc
     2581 gtataaggca tagccagaga tcacaactgc cgtataggga tcgccgtccc cggcttcccg
     2641 cacgccttcg atcttggcca gttcgctcgt accttcgccc agg
//
LOCUS       DATDPQ010000260         6182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1702066, whole genome shotgun sequence.
ACCESSION   DATDPQ010000260 DATDPQ010000000
VERSION     DATDPQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702066"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VI520_05855"
     CDS             complement(<1..1500)
                     /locus_tag="VI520_05855"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HLF37438.1"
                     /translation="MIHNPVTLLTQEQVERIHEASLEILANIGLLVRNEKARRIFEQH
                     GCRVDSETQVVRLPAAVVEEFRASIPPSFTFAGRDPQFDRAIPADGPLIVTGSSAPNI
                     IDPLTGHERRARSDDIARIAHLVNELPGFDIFSISTLADDAPPGMFSVSRFYPALKYC
                     AKPLRGSGPPEDTPQILQLAYLIAGSEAAYRAHPFITHHYCPVVSPLTMDFDSTEVMI
                     YFSEQGLPCYPTIVPNAGLTSPLTLTGTLAQGNAEFLAAAVLEQMVRPGKPTIYSSLP
                     TVADMRTGAYAPGGIETGILFIGIAQLARFYNVPCGGYIGLTNAKLNDAQSGFETGMS
                     TLGGVLAGVDMLNMAGLLDALMTFDFGKAYIDHEIALMLKKAKRGLEFSEQELALDIL
                     AEVGPAGMFADHLHSLERMRTTGLLPEIADRDPRAQWIEKGALDSQARAMRRVQDILT
                     QESSVVFAPEVDARIRAEFVGLIAGELTAPAGWKRQAAPESAGGERRRRR"
     gene            complement(1513..2286)
                     /locus_tag="VI520_05860"
     CDS             complement(1513..2286)
                     /locus_tag="VI520_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLF37439.1"
                     /translation="MESGPPIAIFDPPVNRLVAALPRYVQIAESLLEQIESGQLKPGD
                     RLPAERALSEMLGVNRMTVRRALRVLESRGLVVRLQGDGTYVAEPKIERQAARLTSFT
                     VGMQRRGLQPGARIVSLEQRPVEASVGSLLQLPISAPVYSLLRLRSINQEPVMLERYT
                     IPIQRFPGLERHDLEQRSVYEVFQTEYRVTVERARQSLEPVIATEFEAELLAIPSGSP
                     LMLERRLSFDSDGRPVEHGTDLYRGDRFRFVTELAPLEM"
     gene            2394..3104
                     /gene="nth"
                     /locus_tag="VI520_05865"
     CDS             2394..3104
                     /gene="nth"
                     /locus_tag="VI520_05865"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLF37440.1"
                     /translation="MRAPRQLARQVHQRLLKAYGEPTWRNRKPPVDELVSTILSQNTN
                     DLNRDRAFEALRARFPTWDAVRDAKPSQVVRAIRVAGIANQKGPRIQAALRAITDERG
                     RIELDFLRKQAPEAVREWLLRIKGVGPKTAAIVMQFALDMPAFPVDTHIHRVTGRIGL
                     RAPKASREQAHQILAELFAPEMYYAAHLNLIRHGREICKAARPRCEQCVLQDVCAYYR
                     TVFAKAGKKGRKTTARGW"
     gene            complement(3167..3637)
                     /locus_tag="VI520_05870"
     CDS             complement(3167..3637)
                     /locus_tag="VI520_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HLF37441.1"
                     /translation="MLEGNKAYARRSGKITFPLFTLVLLLSLIILVGPAAAGGRPLSA
                     VLTGPAEVPTGDPDGSGSAFVTLNQGQGEVCFELQVLDIAPATAAHIHVAPAGVAGPI
                     VVPLAPPTTGFSSGCVADVDPDLIKAIRQNPSAYYVNVHNAVFPAGAVRGQLSK"
     gene            4031..4714
                     /locus_tag="VI520_05875"
     CDS             4031..4714
                     /locus_tag="VI520_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLF37442.1"
                     /translation="MSFSLTWYGHAALGLDINGIKVAVDPFLNGNPAATIKPEQVQAD
                     YILITHGHGDHVGDAIAIAKRTGATVVSVNEIAVWFQKQGLKSHGQHLGGAHQHAFGY
                     LKLTLALHGSGLPDGSYGGNPCGLLITAKSGEKVYLAGDTGLFGDMRLIGEEALDLAV
                     LPIGDNYTMGPDDALRAVKLLEPKHVIPIHFNTWDLIAQDPEAWARRVKAETTAEPHV
                     LKPGQTFSL"
     gene            4810..6015
                     /locus_tag="VI520_05880"
     CDS             4810..6015
                     /locus_tag="VI520_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLF37443.1"
                     /translation="MATNTLDPALLSYKAALLAPDHRNTALGLTTAAGLLVATFTQPL
                     IGALSDRTRSRWGRRVPFFLLGTVLSIASLFAVALAPSLALLALAFMGYEFASNTALA
                     PWQALLPDQVAPSRRGAASGLKTMFEILAFVAGRRTAGYLIADDQILASVAVAALVFG
                     LSMFLTALAAREGRGSNPAEEIGSQLPRAPSAAASKTPWPPGFGWWFLNRGLFWGGLI
                     ALSSFVLFYLEDVVGMSFAEASRFFGDLSLVLGLSLLAVALPAGRLSDRIGRRPLIVA
                     SCLIAILGNAVLLTARSRPTLILGGGLLGLAAGTYLASNWALATDLVPRFQAARYLGI
                     ANIATAGGSFLARFAGGALIDPINRTFESSSAGYFTLYGLTLIGFLLATLAVLRMPKA
                     AFFTAQPIE"
BASE COUNT         1105 a         1975 c         1986 g         1116 t
ORIGIN      
        1 ccgccgccgg cgccgttcgc cacccgcgct ctcaggggca gcctggcgct tccacccggc
       61 gggggccgtc agctcgccgg cgatcaagcc cacaaactcg gcccgaattc gagcatccac
      121 ctccggagca aaaacgaccg agctctcctg ggtgaggatg tcctgcaccc gccgcatcgc
      181 ccgagcctga gagtcgaggg cccctttctc tatccactgc gcgcgcggat cccggtcggc
      241 gatttcggga agcaatccgg tcgtacgcat tcgctccaag ctgtgcaggt gatcggcgaa
      301 catccctgcc gggccgacct cggccaggat gtcgagggcc agttcctgct cgctgaactc
      361 cagcccgcgc ttggctttct tcagcatcag tgcaatctcg tgatcgatgt aggccttgcc
      421 gaaatcaaag gtcatcaggg cgtccagcag cccggccata ttcagcatgt ccaccccagc
      481 cagcacgcct cccagggtgg acatcccggt ctcaaacccc gactgggcat cgttgagctt
      541 ggcgttggtc aggccaatgt accctccgca cggcacgtta tagaagcgag ccagctgcgc
      601 gattccaatg aacaggatcc cggtttcgat tccgccgggc gcgtaagctc cggtgcgcat
      661 gtcggcgacg gtcggcaggc tgctgtaaat ggtcggtttc cccggtcgga ccatttgctc
      721 gagcaccgcc gcggccagaa attcggcgtt gccttgggcc aaggtcccgg tcagggtgag
      781 cggggaggtc agcccggcgt tagggacgat ggtggggtaa cagggaaggc cctgctcgct
      841 gaaatagatc atcacttcgg tcgagtcgaa gtccatcgtg agcggcgaga ccaccgggca
      901 gtaatggtgg gtgatgaagg gatgggcccg gtaggctgct tcgcttcccg cgatcaggta
      961 agccagctgc aggatctggg gggtatcttc cggggggccg ctcccgcgga gcggcttggc
     1021 gcagtatttg agcgccggat agaagcgaga cacgctgaac attccgggcg gggcatcgtc
     1081 ggccagagtg gaaatcgaaa agatgtcgaa gccgggcagt tcgttgacca ggtgggcgat
     1141 ccgagcgatg tcatccgagc gggcccgacg ttcgtggccg gtcaaaggat cgatgatgtt
     1201 gggagcggag ctgccggtga cgatcagcgg gccatcggcg ggaatggcgc ggtcgaactg
     1261 cgggtcgcgg ccggcgaagg tgaagctggg cgggatggag gcccggaatt cctcgaccac
     1321 cgcggcgggc agccggacca cttgggtttc ggagtcgacc cggcagccgt gctgctcaaa
     1381 gatgcggcgg gctttctcat tgcggaccag cagcccgata ttcgccagga tctcgagcga
     1441 ggcctcgtgg atgcgctcga cctgctcttg ggttaataac gtcacgggat tatgaatcat
     1501 cgcaagcctc ccttacattt caagcggagc gagctcggtc acgaagcgga atcggtcacc
     1561 ccggtacaag tcggtgccat gttcgaccgg tcggccatcg gagtcgaatg agagccgccg
     1621 ctccagcatc aggggcgaac cgctcggaat ggccagcagc tcggcctcga actcagtggc
     1681 gatgaccggc tccagactct gccgagcgcg ctcgaccgtg actcggtatt cggtctggaa
     1741 cacttcgtag accgagcgct gctccagatc gtgccgctcc agcccgggga agcgctggat
     1801 ggggatggtg tagcgctcga gcatcaccgg ctcctggttg atcgagcgca gtcggagcag
     1861 ggaatacacc ggagcggaga ttggcagctg caacagcgac ccgaccgaag cctcgaccgg
     1921 gcgctgttcc aaactcacga tgcgggcccc gggctgcagc ccgcggcgct gcatccctac
     1981 cgtgaaggag gtcagccggg ctgcctggcg ctcgatcttc ggctcagcaa cgtacgtccc
     2041 atcgccctgc aaacgcacga ccagaccgcg ggattccagc acccgcaggg cacgccgcac
     2101 ggtcatccga ttgacgccca gcatctcgct cagggcgcgc tcggccggga gccggtctcc
     2161 cggcttcagt tggccggact cgatctgttc aagcagcgac tcggcgatct ggacgtaccg
     2221 cgggagagcg gcgaccagcc ggttgaccgg gggatcgaag atggctattg gcggccccga
     2281 ttcaataagg gtctgcttgg actactggtg tataccactt gctccagtat agtagctttg
     2341 gcgaccggct gtcaatcggc acgctttgcc tatgcccttg gtagaatgct cgcgtgagag
     2401 cgccccgcca gctggcccgg caggttcacc agcggctgtt gaaggcctac ggcgaaccta
     2461 cctggcgaaa ccgcaaaccg ccggtcgacg agctggtctc gaccatcctc tctcagaaca
     2521 ccaacgacct taaccgagac cgggccttcg aggccctccg ggcccggttc ccaacctggg
     2581 acgccgttcg ggacgccaaa cctagccagg tcgtgcgtgc catccgagtg gccgggattg
     2641 ccaaccagaa agggccccgc atccaggccg cgctgcgagc gatcaccgac gagcgggggc
     2701 ggatcgagct ggacttctta aggaagcaag ccccggaggc ggtccgagag tggttgctga
     2761 ggatcaaggg ggtcgggccg aagaccgctg cgatcgtgat gcagttcgcc ctcgacatgc
     2821 cggcctttcc ggtcgacacc cacatccacc gggtgaccgg ccggatcggg ctgcgtgctc
     2881 cgaaggccag ccgcgagcag gctcaccaga tcctggccga gcttttcgct ccggaaatgt
     2941 attacgccgc ccacctaaac ctcattcggc acggacgcga gatctgcaag gccgcccgcc
     3001 cgcgctgcga gcagtgcgtg ctgcaagatg tgtgcgcgta ttaccgaacc gtcttcgcaa
     3061 aagcagggaa gaaggggaga aaaacgacgg cgcggggctg gtaaagcagt agtcgaaata
     3121 ggccgacttc cgaattcgga ggacttcgga agtcggcttg gagcgcttac ttcgagagct
     3181 gtccccgcac ggctcctgcc gggaacaccg cgttgtgcac gttcacgtag tacgcagacg
     3241 ggttctgacg aatcgccttg atcaagtcag ggtcaacgtc tgccacacat ccgctggaga
     3301 aacctgtcgt gggtggtgcg aggggcacca caatgggccc ggcaacgccg gcgggagcaa
     3361 cgtgaatgtg ggcggccgtg gccggcgcga tgtccaagac ctggagctca aagcacactt
     3421 cgccctgacc ctggttgagc gtgacaaacg ctgatccgct tccgtcagga tcaccggtag
     3481 gaacctcagc tggccccgtg aggacagcag acagcgggcg acccccggct gcggccgggc
     3541 cgacgaggat aatgagcgac agcagaagca cgagggtgaa cagcgggaat gttatcttcc
     3601 ccgacctgcg agcataagct ttgtttcctt cgagcatggg taagcctcct tcagcgaatg
     3661 gagcgcgacg cctagcggat cgcgccagcg gagatggaaa cgacggtcga gcaactcaaa
     3721 ttctcacctc ctgattattt gctcttccta agggtagcta tgttcgggga tcgccgagcg
     3781 tattgcgtct catccatggg atacgccgtc gcccgcgttt ttggatctat cgcgccgaat
     3841 ctggctgatc cagcctcccg tgagcgcggt caccgggtgg ccggcgcgag gcggattagc
     3901 gcactttacc agtgaatggc ctcgcatatt ctttgccgga cctaggcagc ttcaccgctt
     3961 gatgcacgcg gcctcaaatc gctctatact cggggcagcg gtacgcaagc tggagggaga
     4021 ggaggtgacg atgtccttca gcctcacctg gtacggccac gccgcgttgg gtctggacat
     4081 caacgggatc aaggttgcgg tcgatccctt tctgaacggc aatccggccg ctacgatcaa
     4141 acccgagcag gtgcaggccg attacatctt gattacccat ggccacggag atcacgtggg
     4201 ggacgcgatt gcgatcgcca aacggaccgg ggccacagtg gtcagcgtca acgagatcgc
     4261 ggtctggttc cagaagcagg gcctcaagtc tcacggtcag cacctgggcg gcgcccatca
     4321 gcacgcgttc ggctacctca agctaaccct ggcgctgcac ggttccggcc tgccggatgg
     4381 atcgtacgga ggcaacccct gcggactgtt gatcacggct aagagcgggg agaaggttta
     4441 cctggcaggc gacaccggtc tgttcggcga catgcggttg atcggtgagg aggcgctgga
     4501 cctggcagtc ttgccgatcg gcgacaacta caccatgggg cccgacgacg ctctgcgggc
     4561 ggtcaaactg ctggagccca agcacgtgat ccccatccac ttcaacactt gggacctcat
     4621 cgcccaagac ccggaggcct gggccaggcg ggtgaaggcc gagaccacgg ccgaaccgca
     4681 cgtgctcaaa ccgggccaga ccttcagcct gtagccaagc tgagccaacc cactcctgct
     4741 cgtgatcgtc ccgccgaccc tggctggctg cgcctggccg gcctgacgtt attcgcgctc
     4801 ggcttcggca tggcgaccaa caccctggat ccggccctgc tcagttacaa ggccgcgctg
     4861 ctcgcgccgg atcatcgcaa caccgccctt ggcctgacca ctgccgccgg gttattggta
     4921 gcgacattca ctcagccgct gattggagcc ctctccgatc ggacacgctc gcgctggggc
     4981 cggcgggttc ccttcttcct cctgggaacc gtgctctcga tcgccagtct gttcgccgtc
     5041 gcgctggccc cctcgctggc gctgctggcg cttgccttca tggggtacga gtttgcctcc
     5101 aacacggctc tggcgccctg gcaggcactg ctgccggacc aagttgcacc ctcaaggcgg
     5161 ggggccgctt ccggtctgaa aacgatgttt gagattctgg ccttcgtcgc gggccggcgg
     5221 accgcaggtt acctgatcgc tgatgatcag atcctcgcct ccgtcgcagt tgccgcgctg
     5281 gtgttcgggc tgtctatgtt tttgacggcg cttgcggcgc gggagggtcg tggctccaac
     5341 ccggccgaag agatcggaag tcaacttccg cgcgccccgt ccgccgccgc cagcaagacc
     5401 ccgtggccgc ccgggtttgg ttggtggttc ctcaatcgcg ggttattctg gggcgggttg
     5461 atcgccctca gcagcttcgt gctcttctac ctggaagatg tggtggggat gagcttcgcg
     5521 gaagcctctc gattctttgg tgacctctcg ttggtgctgg gcctctcgct gctggcggtt
     5581 gccctgccgg ccggccgcct atccgaccgg atcggccgcc ggcctctcat cgttgccagc
     5641 tgcttgatag ccatcctggg caacgccgta ctgctgaccg cgcgcagccg gccgaccttg
     5701 atcctgggcg ggggactgct gggactcgcc gccggtacct acctggcctc caattgggcg
     5761 ctagccaccg acctggtgcc ccgcttccag gcagctcgct atctggggat cgccaacatc
     5821 gccaccgccg gcgggagttt tctcgcccgc ttcgcgggag gcgccctgat cgacccgatc
     5881 aatcgaacct tcgagagcag ctcggccggc tactttaccc tctacggcct caccctgatc
     5941 ggctttctgc tcgccaccct ggccgtgctg cggatgccaa aagccgcctt cttcaccgcc
     6001 caacccatcg agtgaatccg agcatgagcg gcaggccgcg tcccagtctg gccgacgcgg
     6061 cggggtgtag aatcgaatca accgcacgaa accaagggga tcaataagga ggccagatgg
     6121 ccgacgaaca gcgcatcggg aaggtcgtcc acttcttcga taagatcggt gtggcagttg
     6181 ta
//
LOCUS       DATDPQ010000261        13642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_646816, whole genome shotgun sequence.
ACCESSION   DATDPQ010000261 DATDPQ010000000
VERSION     DATDPQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646816"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..3145)
                     /locus_tag="VI520_05885"
     CDS             complement(197..3145)
                     /locus_tag="VI520_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLF37444.1"
                     /translation="MAESQALVELSDKLRSRLAGEIHLDPFYRSLYSTDASNHSVQPL
                     GVVIPRNDEDLLAVVETAAEFGIPVIPRGGGTSLSGSAVGAALILDCSQHLAQIHHID
                     PIAQLAEVGPGVVCAQLNHAAAEHGLAYGPDPASADRATVGGMIGTNATGAHSIRYGM
                     TADHVISLDVILADGSPVRLEDISLGQAGAKSQLTSLEGRIYARGLDLRLRYARAVEQ
                     AWPRTWRRSSGYNLNYLTGFPPDRPPAWYGDPEGYPPASEFNLAPLVCGSEGTLAVVR
                     RAQLRLVRKPPFTTLVILGFAGVAEACDFTPAILETEPAAVELIPRTLIQRARAVPAY
                     SRKLGFVEGDPAALLVVEYSGNSPREAEARAQSLAGRGRVLGGAEQQADLWAVRKAGL
                     GLLMSVPGDAKPITFMEDVAVPVGQLGAYVREVDRILAEHGTYGEWYAHASAGCLHMR
                     PLIDLKSDSGVQRMRAIAEAVVNLAISMRGAISGEHGDGLSHTEFNERLFGPELLGAF
                     RELKQAFDPQGIFNPGKVVPDPAQRTKLETQLRFGPEYRATAPATVFAFRKEGGFAKA
                     VEACTGVGICRKADGVMCPSFQATRMEMHSTRGRANALRAVISGGLPKSALSSRELFE
                     ILDLCLECKGCKSECPTEVDMARMKAEVLNWYQQAHGTPLRSRLFGEIATFMGWVRPF
                     AGLTNPIARSRPFRWAMETGMGVARQRVLPPLSRHTFSSQLERSRRRTDGEPVVLFVD
                     TFTELNHPELGHAAVSVLEALGCRVLLAVGQGCCGRTMISKGLLARARERANQNVRAL
                     GKYAKQGVPIVGLEPSCLLMLRDEYLDLLPGDEDAIAIAATARLIEEFLTEVRPAGSS
                     RLAGLDLSPRPGKVLLHNHCHSKALVGSGPALALLQALRSEVEEIQSGCCGMAGSFGY
                     EVEHYALSMQIGELKLFPRIREAAAGSPELQIVAPGMSCRSQIRDGTGATAFHPVTML
                     AEALADHVPG"
     gene            complement(3138..4457)
                     /gene="hflX"
                     /locus_tag="VI520_05890"
     CDS             complement(3138..4457)
                     /gene="hflX"
                     /locus_tag="VI520_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLF37445.1"
                     /translation="MTKHALPSRPPRERALLVGTAWKGQDYLLSMDDSVAELGLLAAT
                     AGLAVVGKVSQRLERPDPKTYVGSGKVSEIRNQAEESLADVVLFDEELSPRHQRELEE
                     AFGEEVRVLDRTALILDVFAQHAHTREGALQVELAQYEYRLPRLTRAWTHLARQAGGG
                     AGRTGSIGGVGLRGPGETQLEVDRREISRRITHLKGELEKVRAHRGRHRAQRRNSQTP
                     IVALVGYTNAGKSTLLNRLAGSEVYVADQLFATLDPTTRRLKMPGGRMILMTDTVGFI
                     QKLPTTLVAAFRATLEEIAEADMLLHVVDVSHPSAEAQAEAVNETLREIGAGQIPVLT
                     ALNKVDRMPSRDLEPQAGIPISAKTGEGIPELIAAVGHELYEVMAPIRVELPYREARL
                     ISLFHEQGKVDAEVHSNGVVKIRGRLPRRLLWAFQDFREGVAEAHLG"
     gene            complement(4454..5836)
                     /gene="trmFO"
                     /locus_tag="VI520_05895"
     CDS             complement(4454..5836)
                     /gene="trmFO"
                     /locus_tag="VI520_05895"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HLF37446.1"
                     /translation="MRTIQVVGGGLAGSEAAWQAAQRGHRVVLFEMRPLSPTGAHTGG
                     DLAELVCSNSLGSQLPDRASGMLLAELEQMGSLLVECARASAVPAGGALAVDRQAFSA
                     QVTRRLLEHPGIELIREQVKSIPEGVAVIASGPLTSERLAAELIRLSGEENLYFFDAI
                     SPIVEAGSVDRSIAFRESRYGRGVSDQGDYLNCPMNKAEYVDFVAALLSAERIPLRSF
                     ESAIESGVRAGADRYFEACLPIEVLAERGEQSLAYGPLRPVGLRDPRTERRPYAVVQL
                     RQDDLAGSLYNLVGFQTNLKFPEQRRIFRMIPGLQSAQFARYGQMHRNTFVNSPKLLL
                     ATLQLRTRSELLLAGQITGVEGYLGNIGTGLLAGLNAARLMEGEPPLVLPPETMLGAL
                     CNYVSHADPGNFQPMKANFGILPPIGSQTSRDRRGRARAFVERGAAALQAAELGSKAE
                     MRIAMPIGSS"
     gene            5911..7305
                     /locus_tag="VI520_05900"
     CDS             5911..7305
                     /locus_tag="VI520_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37447.1"
                     /translation="MLQFQDHSLRTVATAHLAQTMSMLALSNEELKDKVMEELANNPA
                     LELTEEIVCPNCHTRLATRRPCPRCSAATGDQDSVVFLSAREALGRNGKGHLGEDGED
                     LEPVAPESLAIYVLQQLAPDLEPAERPLAAYVLSSLDEEGFLAEPPALIARATRCSLV
                     QVERVLALVSHCDPAGLATAGPRQALLAQLDLFPPDSTGAQLARRVLQVGFESLASRD
                     YETIARGLGVRVGQVRKAAQFIQANLNPFPARADWGSGRSPKNADSNVFHAPDIQISQ
                     QSAGPMMVEIFSSFAGWLRVNPDFRKALPEADQDQSEAWKSHLERASLFVKCLQQRNN
                     TMRKLMEEIVGKQREFILEGDRKLLPMTRAELATRIGVHESTVSRAVANKGIALPDGR
                     IIPLARFFDRSLSVRDRIKELIGQELKPLTDDEIAAVLIQDGVRIARRTVAKYRSIEG
                     ILPARLRKKREVPA"
     gene            7318..9249
                     /locus_tag="VI520_05905"
     CDS             7318..9249
                     /locus_tag="VI520_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37448.1"
                     /translation="MGVAVTSLTRFLRPYPHRELFGALADSVEDGVLVLDGMGSSILA
                     VNHAFLLLTGYTRKELKRLTAEALFPPDTRFADSPGVPVPKLAVRTHAGDSLRLDVWP
                     YPIGNPAAAILLKVRSSAEGERSLRQARVEEDRLAHLIEISTQIAESPMDPKSTVFGL
                     AKQVLSAEFVGVYRASPHSPDYLLDGPLPREFPQSLPPVSLDPLDRPSLWSIGTRPDH
                     PLHKAARAVGLKALRSTPIGSPNAWIGLFVAGWQDAADLPKDVIGLMEVLANLYHAKI
                     LMGMHREVLADLEGQLDRVRRELSSSLQAVHEGMISVDTNLRVLRANSAACQILGYPE
                     GQLKGQAIQDVLVGSADVMTTLLDSLGHQLTAERAQLMLHRRDGRPFPASLRAVPQEE
                     GQDSRLILVLNDHSERKAIEDQTETLAQRALLGEVAAIFAHEVRNPINNISTGLQLVA
                     SRIGQDHPQHDALETIRGECKRLDQLLGDVLFFARPLELKFTPLSLADLVDRILARWE
                     PRLQLAKVGTHTNYANGGTRVLADERTIEQVIVNIVTNSVQAMADGGTLSVHITADDA
                     GGQVLKIADTGPGIRPEQLDRIFDPFFTTKKSGTGLGLAISRRIMTAHQGSIAVESFQ
                     DAGTVFTLQFPLAPSDNGE"
     gene            9246..10424
                     /locus_tag="VI520_05910"
     CDS             9246..10424
                     /locus_tag="VI520_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLF37449.1"
                     /translation="MSATVLVVDDEQTARNFVGGALGDAGFEAIEAGDLAQARQAIAT
                     GAADIVLLDVVLPDGSGLSLLQQIALENPSPPVILITAFGEVDLAVQAMKQGALDFLQ
                     KPLDLEQLIGAVQRANEVVSLRRELAMLRKSSQDQFEWIIGEAPAMVRIADEARRAAA
                     AAASVLITGETGTGKEVLAQVIRHSGPRADKPFVPVNCAALPDTMIESELFGHEAGAF
                     TGATKRKPGLMEVADTGILFLDEISSTKPDMQAKLLRVLDEQRFRRIGGVNELHVDVQ
                     IIAASNRNLRSMMEQGTFREDLYYRLKVVDLHVPPLRDRIADIPSLVGSFIRQLNLRT
                     GNVVSGITPRAIDALKAHDWPGNIRELRHAVERAMLFCDDEELDVGHFSPEFQALIPS
                     "
     gene            10551..10880
                     /locus_tag="VI520_05915"
     CDS             10551..10880
                     /locus_tag="VI520_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37450.1"
                     /translation="MIPFTVIDMQVVIGSAVFVLGCMCVLLGIFVLVTRGYSRDIRAL
                     ANHTARLGQKGMAEEITGLVRSATELVGALSNLVKTASGAGLFLITLGMVMIASAYWV
                     VTQIQTA"
     gene            10893..>13642
                     /locus_tag="VI520_05920"
     CDS             10893..>13642
                     /locus_tag="VI520_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37451.1"
                     /translation="MHTDQAVLKALAGHYSVEQLAAVLGRILRVPEAWAALHNPGFLA
                     EVMASSPGPQITPSQLAALALGGSLAEPIHTALADPHRERAELAWQEAELRAGGSRDL
                     FEAALIGLEFVRRSAGSGDPSSLIERACRTPEVWSSPLALIWPTLPDPDSLLAQVIQR
                     DRAELAIQIALSNLPVAEAGAALFRNAGDSSLQLIQAIHESGETELAGCIPELGVIRL
                     KNPLLEPIAKSGDDRPDRNHDSTHRLLSEAWQATTSAAALVADLTAAHARIDGDPVTE
                     LEAIRHALGLHPTPARRAWAALSLMKLGRFGEAMDTLPRGQPSIEEQIAHGLILTQHG
                     ESGEAELRAALAGVATGSRLEEAWFELLASGLWSSGERTSAIEAATTRSQRFPAKTAA
                     RAGLGRLLFEAGDSEASRHQSALALALSPSSRAARQLLATSLQDCGEFTEALSHWKIL
                     AIEGAGDPEALVECALRAGDTDAASAAVAKQLEQDPTSRTARIIEGRILVAGGSVAAG
                     REKIEAAIELGPQTAGPYLALANLQKQTGDPLESGKTLARGVQATPGSAEIRVALAKW
                     LSSQGRLSEGLETAASATALGSDNLAAVLVEADLLRQLGHLDQAIANLRQATRLKPLS
                     WRVGLALAEALVARGEFEEAARLAAAVPASAPPEALLTAGKVGVMAGIQLETALRMLT
                     QADTAGLSSPDLDFWLGRGLESDGQFGPAIVRFEQALAQLPVELVELREAATLGVARC
                     ALGAGQISFALTTLESALEWFTGSTEILALMSKVYQAANLPEKAVEVARQAVDGNQES
                     VAGWSALSKALALSDDFEAALQSLREMSSRHPTSTAAWLELAEVALARGNPTIGRRAA
                     AEVAWRGRRRPEVLASLASLLGNHGSRPTALRLLRTGLRIRPHDVRLRRQLAELKEAA
                     GD"
BASE COUNT         2555 a         4374 c         4223 g         2490 t
ORIGIN      
        1 gcactatttc gtcacgaccg tgctcagggg ttccggcgga aacctgaagc tggcccagga
       61 gctcgcccgc catcggagca tcgcggttac ccagcgctat gcgcacctct ccgacgacga
      121 gctcgatcgg gggtactacg atatcttcga atccggccaa gcggttgcca aggattgagc
      181 tcgactcatc gaccactcat cccgggacgt gatcagccag cgcctcggct agcatcgtca
      241 ccggatggaa ggcggtagcc ccggttccat ctcgaatctg cgagcgacac gacatcccgg
      301 gggcgacgat ctgaagctca ggagacccgg cagctgcttc gcggatccgg gggaacagct
      361 tcaattcccc gatctgcatg gacagcgcgt agtgctcgac ctcgtacccg aacgagcctg
      421 ccatcccgca gcaccccgac tggatctcct cgacctctga ccgaagcgcc tgcagcagtg
      481 cgagcgccgg cccgctgccc accagtgcct tggaatgaca atggttatgc aacaggactt
      541 tgccggggcg cggagagagg tcgagacccg ccaggcgact gctccccgcc ggcctgacct
      601 ccgtcaggaa ctcctcaatc aatcttgcag tggctgcaat cgcgattgcg tcctcatcac
      661 cgggcagtag gtccaggtac tcgtcgcgca gcatgagcaa gcacgaaggc tcgagcccaa
      721 cgatcggaac gccctgtttg gcgtatttcc ccagggcccg aacgttctga ttggcccgct
      781 ctctcgcccg cgccagcaga cccttcgaga tcatggttcg cccacaacac ccctggccga
      841 ccgcgagcag cactcggcaa cctagcgctt ccagcacact gactgcagca tgccccagct
      901 ccgggtgatt cagctcggtg aacgtatcga cgaacaagac aactggctct ccgtcggtcc
      961 tccggcgact tcgctctaat tggctcgaaa aggtatgccg ggagagcggc ggcagaaccc
     1021 gttggcgggc gacgcccatg ccggtctcca tcgcccagcg aaaaggtctg gaccgtgcaa
     1081 tcggattcgt caaccctgcg aaggggcgaa cccaccccat gaaagtcgca atctccccga
     1141 agagcctgct ccgcagcggt gttccgtgcg cttgttgata ccaattcaag acctcggcct
     1201 tcatccgcgc catgtcgacc tcggtcgggc attccgattt gcaacccttg cattccaggc
     1261 agaggtccag gatctcaaag agttcgcggc tgctcagggc actcttcggc aggccgccag
     1321 agatgacggc tcggagggcg ttcgcccgcc ctcgggtcga gtgcatctcc atccgggtcg
     1381 cctgaaagga ggggcacatc actccgtcgg ccttacggca gattccgact ccggtgcagg
     1441 cttcgacggc cttggcgaag ccgccctcct tccgaaatgc gaaaacggtc gcgggggcag
     1501 tggcccggta ctccgggccg aagcggagct gcgtttccag ctttgtgcgt tgcgcgggat
     1561 ctggcaccac ctttcctggg ttgaagatcc cttgaggatc gaatgcctgc ttgagctcgc
     1621 ggaaggctcc gagcagttcg ggtccaaaca gtcgttcgtt gaattcggta tgggagagcc
     1681 cgtccccatg ttcacccgag atggcacccc gcatagagat ggccaggttc acgaccgcct
     1741 ccgcaattgc ccgcattcgt tggacgccgg agtcggactt gagatcgatc aatggccgca
     1801 tgtgcaggca gccggccgag gcgtgcgcgt accattctcc gtatgtacca tgctccgcca
     1861 ggatccgatc cacctctcgg acataggcac ccagctgccc caccggcacc gccacatcct
     1921 ccatgaacgt gatcggcttg gcgtccccgg ggaccgacat gagcaaaccg aggcccgctt
     1981 tgcggactgc ccacaggtcg gcctgctgct cggctccgcc gagcaccctg ccccggccgg
     2041 ccaacgactg cgctctggcc tccgcttccc tgggactgtt gccggagtat tcgacgacca
     2101 agagcgccgc tggatccccc tcaacgaaac cgagcttgcg ggagtatgcc ggaacggccc
     2161 gggcgcgttg gatcagagtt cgcggaatca gctccaccgc ggccggttcg gtctccagaa
     2221 tggccggagt gaaatcacag gcctctgcca ctcccgcgaa gcccagaata accagggtgg
     2281 tgaatggggg tttgcggacc aatcgcagtt gcgcacgtcg gaccaccgcc agggtgccct
     2341 cagacccgca aaccagcggt gcgaggttga attcgctcgc tggcgggtac ccttccggat
     2401 ccccatacca ggccggcggc cgatccgggg ggaagccggt gaggtaattc aggttatacc
     2461 cggaggatcg gcgccaggtc ctcggccaag cttgctcaac cgcccgcgca tagcgaagcc
     2521 gcagatccaa cccgcgggcg tagattcggc cctctaagga ggtcagctgc gatttggctc
     2581 ctgcctgccc aagactgatg tcttccagcc ggaccgggga tccatcagcc aagatcacat
     2641 ccagagaaat cacgtgatcg gcggtcatgc cataccgaat cgaatgcgcc ccggtcgcgt
     2701 tcgtgccgat catgcctccc acagtggctc gatcggccga ggccggatca gggccgtagg
     2761 caagcccatg ctcggcggcc gcatggttga gctgggcgca gaccacccct ggcccaacct
     2821 cggcgagttg agcgatggga tcgatgtgat gaatctgagc caggtgttgc gaacagtcga
     2881 ggatcagggc tgctcccact gcagaaccgg agagactggt cccacctccc cgcggaatca
     2941 ccggaatgcc aaactcagcc gcggtctcga cgaccgccaa caagtcctcg tcgtttcgtg
     3001 ggattacgac cccgagcggt tggacgctgt ggttcgaggc gtcggtcgag tagagggagc
     3061 ggtagaacgg gtcgaggtgg atttcgccgg ccaggcgtga tctgagctta tcgctcagct
     3121 caaccaacgc ctggctctca gccaagatgg gcttctgcca cgccttcccg gaaatcctga
     3181 aaggcccaga gcagacggcg gggcagcctg ccgcgaatct tgactacgcc gttggaatga
     3241 acttccgcat caaccttccc ttgttcgtgg aatagactga tgagccgtgc ctcccggtat
     3301 ggaagctcaa cccgaatcgg agccatcact tcgtagagct catgtccaac tgccgcgatc
     3361 agctccggga ttccctcgcc ggtcttggcg gaaatcggaa tgccggcttg cggttcgaga
     3421 tcccgggacg gcattcggtc gactttgttc agtgccgtga gaaccggaat ctggccggct
     3481 ccgatttccc gcagggtctc attgacggct tcagcctggg cctcggcgct ggggtgcgag
     3541 acgtccacca cgtgcagcaa catgtcggct tcagcgatct cctccaaggt ggcccgaaac
     3601 gccgcaacca gggtggtcgg aagtttctgg ataaatccga ccgtatcggt catcaagatc
     3661 attcggcccc cgggcatttt gagccgacga gtggttggat ccagggtcgc gaacagctgg
     3721 tccgcgacat agacctcgga tccagccagc cgattcagga gagtggactt gccggcattg
     3781 gtatagccga ccagtgcgac gataggagtc tgtgaattcc ggcgctgagc gcgatgtcgg
     3841 ccccggtggg cccggacctt ctccagctcg cctttgaggt gcgtgatgcg gcgggagatc
     3901 tctctgcgat ctacctccag ctgcgtctcg cctgggccgc gcagtccaac tcccccaatc
     3961 gaaccggtgc gtcctgctcc accaccggcc tgacgagcca ggtgggtcca ggctcgggtc
     4021 aggcgtggaa gccggtactc gtattgggca agctccactt gcagggcccc ctctcgggtg
     4081 tgggcatgct gggcgaacac gtccaaaatc agggctgtcc ggtccaatac ccgcacctcc
     4141 tcgccgaagg cctcctcaag ctcgcgctgg tggcggggcg agagctcctc gtcgaaaagc
     4201 acgacatctg ccagcgattc ctcggcctga ttccggattt cactaacctt tcccgaccca
     4261 acgtaagttt tcggatcggg acgctccagc cgctgggaaa ccttccccac gactgccagc
     4321 cccgcggtcg ccgcgagtaa gcccagctcc gccaccgagt catccatcga tagcaggtaa
     4381 tcctgccctt tccaggcggt acccaccagc aacgcccgct ccctgggtgg ccggctaggc
     4441 agggcgtgct tggtcaagac gagcctatcg gcatggctat gcgcatttct gcctttgacc
     4501 caagctcagc ggcctgcagt gcggcggcac cacgctcgac gaaggctcgg gcccgcccgc
     4561 ggcgatcacg actggtttgc gaacctatcg gcggcaggat accgaaattg gccttcatcg
     4621 gctggaaatt cccggggtcg gcgtggctga cgtagttgca cagcgccccc agcatcgtct
     4681 ccggaggcaa aaccagcggc ggctcgcctt ccatcaagcg ggcagcattc aatccagcga
     4741 gcagcccggt gccaatattc cccaaatagc cctctacccc agtaatctgg ccggccagca
     4801 agagctcgct gcgggttcgg agctgaagcg ttgccagcag cagctttggt gaattcacga
     4861 acgtgttccg gtgcatctgt ccgtagcggg caaactgggc cgactgcaag cccgggatca
     4921 ttcggaatat ccgtcgctgc tcgggaaact tgaggttggt ctggaatcca accaggttgt
     4981 acaggctgcc cgcaagatcg tcctggcgca gctgaacgac cgcatagggc ctccgctcgg
     5041 tccgcggatc tctcaatccc accggccgca ggggtccata ggccaacgac tgctcccctc
     5101 gttcggccaa aacttcgatc ggaagacagg cctcgaaata ccgatccgcg ccggcccgta
     5161 ccccggactc aatcgcggat tcaaaggacc gcagcgggat ccgctctgcg ctcagcaatg
     5221 ccgcgacgaa gtccacatac tctgccttgt tcatggggca gttcaagtag tccccctgat
     5281 cgctaacccc tcgaccgtat cgagactcac ggaaggcgat cgaccggtct acgctaccgg
     5341 cttcgacgat cggggagatc gcgtcgaaga aatacaagtt ctcttctccg ctcagccgaa
     5401 tgagctcggc ggccagccgc tccgaggtca gcggcccgct ggcgatcacg gcaaccccct
     5461 cggggatgct cttgacctgc tctcggatca actcaatgcc agggtgctcg agcagccgtc
     5521 gggtgacctg tgccgaaaag gcctgccgat cgacggccag cgctccgccg gccggcaccg
     5581 cactcgctcg ggcgcactcc acgagcagcg agcccatctg ctccaactcg gcaagcagca
     5641 ttcccgaagc tcggtccggg agctgggatc caagcgaatt cgagcacacc agttcggcaa
     5701 gatctccccc ggtgtgggca ccggttggag ataagggccg catctcaaaa aggaccaccc
     5761 gatggccgcg ttgggcagcc tgccaggccg cctcgctgcc tgccagccct cctcccacga
     5821 cctggattgt ccgcatgcgc ggattgtagc acgctgggag tggcacgctt cttgcacttt
     5881 ccgctatact caccccgctg gagccagaga atgcttcaat tccaagacca ctcgcttcgt
     5941 acggtcgcaa cggcccacct tgcccagacc atgagcatgt tggccttgtc gaatgaagaa
     6001 ctcaaagaca aggtcatgga ggagctggct aacaatccag ccctggagct gacggaagag
     6061 atcgtctgtc cgaactgtca cacccggctg gccacccgcc ggccgtgccc gaggtgcagc
     6121 gcggcgaccg gcgatcagga ttcggtcgtt ttcctttcag ctcgtgaagc cctcggccgg
     6181 aacggcaagg ggcatctggg ggaggacggc gaggatctgg aacctgtggc tccggagagc
     6241 ttggcgatct acgtgctgca gcagttggca cccgatctag agcctgccga gcggccgctt
     6301 gcggcctatg tgctttccag cctcgatgag gaaggattcc tggccgagcc tccggcctta
     6361 attgcccgcg ccacccggtg ctcgctcgtc caggtcgagc gggtcctggc actggtctcg
     6421 cattgcgatc cggccggcct ggccaccgcc ggaccacggc aggcgctgct tgcacagctc
     6481 gatctgtttc cgcccgattc gacaggtgcc caactggcac gccgagtcct acaggtcgga
     6541 tttgaatcgc tggcgagccg agactacgaa accatcgcac gggggttggg cgtccgggtg
     6601 ggtcaggtcc ggaaggcggc ccagttcatc caggccaacc tcaacccctt tcccgcccgg
     6661 gcggattggg gttccggccg gtcacccaag aacgcggaca gcaatgtgtt ccatgcgccg
     6721 gatatccaga tcagccagca gtcggccggg ccaatgatgg tggagatctt ttcctctttc
     6781 gccgggtggt tgcgggtgaa tccggacttc cgtaaggcac tgccagaggc cgaccaggac
     6841 caatcagagg cctggaaatc ccacttggag cgggcctccc tatttgtcaa atgtcttcaa
     6901 cagcgcaaca atacgatgcg caagctcatg gaagagattg ttggcaagca gcgggagttc
     6961 atcttggaag gggatcgaaa gctactgccg atgacccgag ccgagttggc gactcggatc
     7021 ggggtgcatg agtcgaccgt ctcccgggcg gtggccaata aggggatcgc cttgcccgac
     7081 ggtcgaatca ttccactggc ccgctttttt gatcgcagcc tctcggtacg cgaccggatc
     7141 aaggagttga tcgggcagga actcaaaccg ctcaccgatg acgagattgc cgcggtccta
     7201 atccaagacg gcgttcggat tgcgcgccga acggtcgcga agtatcgttc catcgaaggt
     7261 atactcccgg cccgtttgcg gaagaagcgg gaagtgcccg cctaaagggt cgaagacttg
     7321 ggtgtcgccg tcaccagcct tacaagattc ctaaggccgt acccccaccg ggagctcttc
     7381 ggagctctcg ccgattcggt cgaagatgga gtgctcgtcc tggacggaat gggcagctcg
     7441 atcttggcgg tcaatcatgc gttcctgctg ctcactggat acactaggaa agaactgaag
     7501 cgcctgacgg ccgaagcgct cttcccacca gacactcgat tcgccgattc ccctggggtg
     7561 ccagtcccaa agctcgcggt cagaactcac gcaggggata gtctgcggct cgacgtctgg
     7621 ccctacccga tcggcaatcc tgccgcggcg atcttgctca aggttcgatc ctcggccgag
     7681 ggtgagcggt cgctgcggca agccagggtg gaggaggacc gcctcgccca tttgatcgag
     7741 atctcgaccc agatcgccga gtcgccgatg gatccgaaat cgactgtttt cggactcgca
     7801 aagcaggtgc tctcggcgga attcgtcggc gtatatcggg cgtcccccca cagcccagac
     7861 tatctcttgg acggcccgct gccaagggag ttcccccagt cgcttccccc ggtttcgctc
     7921 gacccgcttg accgccccag cctctggtca atcggaactc gccccgacca tccactccac
     7981 aaagccgccc gggcggtagg actgaaggcg ctcaggagca cgccgatcgg ctcacccaat
     8041 gcctggatcg ggctgttcgt ggcgggctgg caagatgcgg cagacctgcc caaggatgtg
     8101 atcggcctga tggaggtctt ggctaacctc taccatgcaa aaatcctgat ggggatgcac
     8161 cgcgaagtac tggcggatct ggagggccag ctggatcgag tacgccgaga gctgtccagc
     8221 tcccttcaag ctgtccatga gggcatgatc tccgtggata ccaacttgag agttttgcgg
     8281 gccaattcgg ccgcctgcca gattctgggt taccccgaag ggcagctcaa gggccaggcc
     8341 attcaagacg tactggtggg ttcggcagat gtgatgacca ccctgttgga ttcactcggc
     8401 catcagctca ccgccgagcg cgcccaactg atgcttcacc gtcgggatgg gagacccttt
     8461 ccggccagcc tgcgagcagt gccccaagag gagggtcagg actctcgatt gatcctggtt
     8521 ctcaatgacc actctgagcg gaaggcaatc gaagaccaga ccgagacgtt ggcgcagcgg
     8581 gccctgctgg gggaggttgc cgccatcttc gcccatgagg tccggaatcc gatcaacaac
     8641 atcagtaccg gcctgcagtt ggtggcctcg cggattgggc aggatcaccc ccaacacgac
     8701 gccttggaaa cgatccgcgg tgaatgcaag cggctggatc agctgctcgg cgacgtgcta
     8761 ttcttcgctc ggccgcttga actcaaattc actcccctga gccttgctga tctcgtggat
     8821 cggatcctcg cccgctggga gccgaggctg cagcttgcca aggttgggac ccatacgaac
     8881 tatgccaacg gtggcacccg ggtgctcgcc gacgagcgga cgatcgagca ggtcattgtg
     8941 aatatagtca ctaatagcgt ccaggcgatg gcggacggcg gaaccctatc cgtgcatatc
     9001 acggcagacg atgccggtgg tcaagtgctc aaaatcgccg acacgggacc ggggatccgg
     9061 cccgagcaat tggatcgcat cttcgacccc ttctttacga ccaagaaatc cggcaccgga
     9121 cttgggctgg cgataagccg ccggatcatg accgcccacc agggcagtat tgcggtggag
     9181 agtttccagg acgcgggcac ggtcttcacc cttcaattcc cgctcgcacc gagcgacaac
     9241 ggcgaatgag tgcgaccgtc ctggtagttg acgacgagca aaccgcccgg aacttcgttg
     9301 gcggtgcatt gggcgacgcc ggcttcgaag cgatcgaagc cggggatctg gcccaggcca
     9361 ggcaagccat cgcaactggc gcggctgaca tcgtcctgct ggacgttgtg ctgccagacg
     9421 gatctggcct ctcgttgctg cagcagatcg cccttgagaa tccgagccct ccggtcattc
     9481 tcatcacggc atttggtgaa gttgacctcg cggtccaggc catgaaacaa ggcgcgctcg
     9541 atttcttgca gaagcctttg gatctggaac agctgatcgg cgcggtccag cgggccaacg
     9601 aggtcgtctc gctccgtcgc gagctggcaa tgctgcggaa gtccagccag gatcaattcg
     9661 agtggatcat aggggaggcc ccggccatgg tgcggatcgc cgatgaggct cggcgggccg
     9721 cggccgccgc cgcatcggtg ttgatcactg gagagaccgg gaccggcaaa gaggtgttgg
     9781 ctcaagtcat ccgccactct ggcccgcggg ccgacaaacc attcgttccg gtgaactgcg
     9841 cggcgctgcc tgacacgatg atcgagtccg agttattcgg gcacgaggcg ggagcattca
     9901 ccggggccac caaacggaag ccgggtttga tggaggtggc agacacggga atcttgttcc
     9961 tggatgagat ctcctccacc aagccagaca tgcaggccaa gctgctcagg gtgttggacg
    10021 agcagcgctt ccgccggatc gggggcgtca atgaactcca cgtagatgtt cagatcatcg
    10081 cggcctccaa ccggaacctg cgctcgatga tggaacaagg cactttccga gaagatctgt
    10141 actaccgcct caaagtcgtc gatcttcatg tgcctccgct ccgcgaccgt attgcggata
    10201 tcccctcctt agtcggatct ttcatccgac agctcaacct gcggaccggc aatgtggtct
    10261 ccgggatcac ccctcgggcg attgacgcgc tgaaggccca cgattggccc gggaatatcc
    10321 gcgagctccg gcacgcagtg gaacgggcaa tgctgttctg cgacgacgaa gagctcgacg
    10381 tagggcactt ttcccccgaa ttccaggccc taatcccttc ctgatcgacc cgctccccag
    10441 ctagccctca accgactgac ctgatcacgg gtccgttccg cccggcgagc ttgcgcgcct
    10501 ggcacgctga ttgcaactca ggcctgagat cacaggaacc ggaggcggac atgatccctt
    10561 tcacagtcat cgatatgcag gtcgttattg gttcggcagt cttcgtgctt ggctgcatgt
    10621 gcgtactgct gggtatcttc gtgctggtca cccggggcta ctcgcgggac atccgggcac
    10681 tggccaacca cacggcccgg ctcggccaga aggggatggc cgaggaaatt accggcctag
    10741 tccgcagcgc gaccgagctc gtgggggctc tgagcaattt ggtgaaaacg gcaagtgggg
    10801 cggggttgtt cctcatcact ctgggcatgg tgatgatcgc ttccgcctac tgggtcgtta
    10861 cccagattca gactgcctga gcggcggtcc gcatgcacac ggaccaagcc gtactcaaag
    10921 cgctggctgg gcactattcc gttgagcagc tggcggccgt gctcggccgg atcttgcgcg
    10981 taccggaggc atgggcggcg ctccacaacc caggattcct cgccgaagtc atggcgtcta
    11041 gcccagggcc ccaaattacg ccctcccaac tagccgccct ggccttaggc ggtagcctgg
    11101 ctgagccaat ccacacggct ttggcagacc cgcatcggga acgggctgaa ttggcctggc
    11161 aggaggctga gctgcgtgcc ggcggtagtc gagacctgtt cgaagccgcc ttgatcggtt
    11221 tggaattcgt tcggcggtcc gcagggtctg gcgatcccag ctccctgatc gagcgtgcct
    11281 gccggactcc agaggtgtgg agctctcctc tggcattgat ctggccgacc cttccagacc
    11341 ccgactcact gctcgctcag gtgatccagc gcgatcgcgc cgaacttgcg attcagatcg
    11401 ccctgagcaa cttgccggtt gccgaagccg gcgctgcgct ttttcgcaac gccggtgatt
    11461 cgagcctgca gctgattcag gccatccacg agagtggaga gaccgagttg gccggatgca
    11521 tcccagagct cggggtcatc cgcctaaaaa acccgctgct cgagccgatc gcaaagagtg
    11581 gcgacgaccg gccggatcgg aaccacgatt ccactcaccg gctgctgagc gaggcctggc
    11641 aggcgacgac ctccgcggcg gctttggttg cggacctgac cgcggcccac gccaggatcg
    11701 acggtgatcc cgttaccgaa ctggaggcca ttcggcacgc attgggcttg catccgactc
    11761 ctgcccggag ggcctgggcg gcattaagcc tgatgaaact gggtcggttc ggggaggcaa
    11821 tggataccct gccccgcggc cagccttcga ttgaagagca gatcgcccat ggcttgatcc
    11881 tcacccaaca tggcgagtcc ggggaagcag aattgcgggc cgcgctcgcg ggagtcgcaa
    11941 ctgggtcgcg gcttgaagag gcctggtttg aattgttggc tagtggcctg tggagttccg
    12001 gtgagcgaac ctccgcaatc gaagccgcca caacccgatc ccagcggttc cccgcaaaga
    12061 ccgccgcgag ggcgggcctc ggtcggctgc tgttcgaggc gggagacagc gaggccagcc
    12121 gtcaccaatc ggccttggcc ttggcgttat ccccgagctc acgagccgcc cgccagctgc
    12181 tcgcaaccag ccttcaggat tgcggcgagt tcacggaggc attgagccac tggaaaatat
    12241 tggcgatcga aggggcagga gatcccgagg cattagtcga gtgtgcgctg cgtgccggcg
    12301 ataccgacgc ggccagcgcg gccgtcgcca agcagctcga gcaggacccg acctcccgga
    12361 ccgcccggat tattgaaggc cggattctcg ttgccggcgg gagcgttgct gccggaagag
    12421 aaaagatcga agcagccatc gagttgggcc cgcaaacggc cggcccctac ctggcgctgg
    12481 ccaacttaca gaagcagacc ggggacccac tcgagtccgg caagaccctt gcccggggag
    12541 ttcaggccac cccgggaagt gccgagatcc gggtggcgct ggccaagtgg ctttccagcc
    12601 aagggcgtct gagtgaagga ttggaaacgg ccgcatcggc gacagcgctg ggttcagaca
    12661 acctcgccgc ggtcctagtt gaagccgatt tgcttcgcca attgggccat ctcgatcaag
    12721 cgatcgccaa tttgcgtcag gctactcggt tgaaaccgtt gagctggcgg gtcgggctgg
    12781 cactggccga ggcgctcgtg gcacgcggcg agttcgagga ggccgcacgc ttggccgccg
    12841 cagtccccgc ctcagcccca cccgaggcgc tcctgactgc cggaaaagtc ggggtgatgg
    12901 ctgggatcca gctcgagacg gcgttgcgaa tgcttacgca agccgacacg gcggggttat
    12961 ccagccctga tctggacttc tggctcgggc gcgggctgga gtccgacggt caattcgggc
    13021 cggcgattgt gcgcttcgag caggccctcg cccagctacc cgtggagctg gttgagctca
    13081 gagaggcagc cacccttggg gtcgcccgct gcgcgctggg tgcggggcag atctcatttg
    13141 cactgacgac cctggaaagt gcgcttgaat ggttcaccgg atcgaccgag atcctggcat
    13201 tgatgtctaa ggtctaccag gccgctaacc tccccgaaaa ggcggtcgaa gtggcaaggc
    13261 aggcagtgga cggtaaccag gagagtgtgg ccggctggag cgccttgagt aaggcattgg
    13321 cgttgtcaga tgattttgag gcggcgctgc agtcccttcg tgaaatgagc tcccggcatc
    13381 caacgagcac tgctgcgtgg ctcgaactgg ccgaggtggc tctggctcgg ggcaacccga
    13441 ccatcggccg gcgcgcggcg gcagaagttg cctggcgtgg tcgacggcgg ccggaagtct
    13501 tggccagctt ggcctctttg cttggcaatc acggctctcg accgaccgct ttgcgactac
    13561 tgcgcactgg gctacggatc cgcccgcacg acgtccggct gcggcgacag ctggccgagc
    13621 tcaaagaagc ggccggtgat ca
//
LOCUS       DATDPQ010000262         8582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2481156, whole genome shotgun sequence.
ACCESSION   DATDPQ010000262 DATDPQ010000000
VERSION     DATDPQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481156"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1341
                     /gene="cysS"
                     /locus_tag="VI520_05925"
     CDS             73..1341
                     /gene="cysS"
                     /locus_tag="VI520_05925"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLF37452.1"
                     /translation="MKLYNTLSREIQEFAPQGSQIGIYVCGITPYDTTHLGHAFTYSV
                     ADVLIRYLEYQGGAVRYVQNVTDVDDDLLRVAGERQEDWRRMGDRWTAHYIEDMAALN
                     IRPPDVLPRATDVMPQIIESVEQLLQAGVAYVAGGSVYFDLKAWPAYGRLSRLPPDQM
                     LPIANERGNRPEDPNKRQPLDFVLWQPQAPGEPAWDSPWGPGRPGWHIECSTMASQLI
                     GQTVDLHAGGEDLAFPHHESEIAQTESLTGKPFAGHWLHVAPVRHLGEKISKSLGNLV
                     MVRDLLDRWPPDAIRVYLASHQYRHSWAYDAEGLYDAAREAQALAAAAALASGEGDSL
                     SVDELVSEFAQAIEQDLNTPAAIAVLRRLAESIVAASNQGGALGAAQDVLRKLGGVLG
                     LRLGSAGPEGKVVEGWRRHLTRFRSEPVPG"
     gene            1363..3654
                     /locus_tag="VI520_05930"
     CDS             1363..3654
                     /locus_tag="VI520_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37453.1"
                     /translation="MQGLIGQELGGYRIIAQIGKGGMATVFKAYQPSLDRYVAIKVLP
                     PYYAEQDETFLKRFRQEAKAIASLRHPNILIVLDYGELERTTYLVMEFVEAGTLGGQM
                     GKPMAPGQMAGLVGQVAGALHYAHQEGVVHRDIKPSNILLPKPNWPLLTDFGLAKIVG
                     GTQLTQSGTVAGTPAYMSPEQGRGERVDHRSDIYSLGIVLYEMATGVVPFQAETPMAV
                     IVKHIIDPLPLPRSKTPELSEAIERVILKALAKEPADRFQDVGQLAEALTAAVGTLPP
                     GKASVAPSFPSHELTTLQVTGSGVAAQLPVATRVDPEEGMAEAVAAGARAKGAKSWWF
                     RLGLAAGVLVLGFVGFAAARALGFGGSAGSPIADEPGEARTLEQLVADGYANLQSGDF
                     EAAIRDFERAIEREPGNPERYFDVARAYFASGDPDAASDLIQEAVDLIPGDSGVHESA
                     GWVYQELGLHEEAASEFRAALELDPQELWLYAAISDSYLAAGLPEEAAGVLAEGLANP
                     EVAADSASLESIGWVFLSLGDPAQAERTFNQAITAGADFPGPWEGLAEAVYQQGELVD
                     ALAILADGMQYFPEHVPFYQTTGELLWEQGELEQAATSFQQAIALDPANSSSYSSLAR
                     MYSEMGLLKEAEQVLLGGLEAHPENTSFYGDLASYYSGQGRYADAVPLYETLTQAEPD
                     NGWWFAYLADTYRNLGDPENARRVLGEAADRSSGDGWLNDFIGWIYVGLGDCDTALGH
                     FEYALELDSSIESSAEGLQSCGG"
     gene            3855..5402
                     /locus_tag="VI520_05935"
     CDS             3855..5402
                     /locus_tag="VI520_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLF37454.1"
                     /translation="MRASIECLPGREIPAGQLLRAVLGLGLALGGLGGLGLALLLGIY
                     GFFRVSDSIAPGVAVGQLPVGGLLMTDAADRLDQAWNQDLVLTAVDTSEAGRTWQVSP
                     GEFGLRVEAVESARLAYAYGRQGGLIPNLIDLVTGFTNSWQIAPVVEFDPSQARTALT
                     TWAEKIALPAADARLRLEAGEVLITQAQWGRRLDLEASLALLSADPGSVLLRYGFIPL
                     VTEPVAPFIAEVEAAAIEADQLLSADIVLRAYDPVTDEWLDWAPNRAEIGGWLLVGQE
                     GGRLTVSINEQAVRAFVGGLPVGDERTLDEEAAAQSLLAGLRGEAGEPILLRYLPRYY
                     QVSPEDTLISISFKVGIPYWKWLESNPQVAARGLVPGESLTIPPRDAMLELPVIQGKR
                     IVISISEQHMWLYQDGELVRENVVSTGIPNSPTMPGLFQVKSHFLNAYASNWDLTMPH
                     FLGIYHAVPNFLNGIHGLPLLSNGVRLWANVLGRPASYGCVILDLEAAEHLYGWAEDG
                     VVVEIRS"
     gene            complement(5408..5899)
                     /locus_tag="VI520_05940"
     CDS             complement(5408..5899)
                     /locus_tag="VI520_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HLF37455.1"
                     /translation="MRLGVLSDSHDDVANLETALADYRRRGISQLIHCGDLIGPEIVR
                     QLKGFQVIYVDGNMDREPAEIYRTLRELDPHNIALPTFEGEIADVSFAVTHGDDLPEL
                     NRLVRSGIHRFVFTGHTHKRRDETIGSTRVFNPGALGGLQFESRSYSVVDLTSGEIET
                     IEF"
     gene            complement(5943..7544)
                     /locus_tag="VI520_05945"
     CDS             complement(5943..7544)
                     /locus_tag="VI520_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLF37456.1"
                     /translation="MPKTTPQYDVVIIGGGHNGLVSAAYLARGGLRVVVLERRDILGG
                     AAATEEVFPSFRFDTGSQTAGMLRPEVQRELRLADHGLQLFESPIRCASLLGPDAALL
                     FWRQPDRASRELAKISKSDAERYPVFLRQFGKLASGLDRAIGLTPPALPPLLSELWGW
                     ARVAVGIRLLGRAGMMELLRTIGLSAENLLDDWFESGPLKGALGSLAVAGSALGPKAP
                     GTAFTLLYLGAPGLPRTASFVLGGTGQLSEALARAARANGAEILTGEPVVRILLDDVR
                     AVGVELRNGKQIRARVVASSADPRSTLFDLVGPSQLEVRVVRRVRSIRFRGTTARVNL
                     ALERLPDFPAIKDPAGLTGHLLLCPDLDYLERAFDDSKYGQFSQRPTLDMTIPTLYDP
                     SRAPSGKHILTITMQYAPYQLRDSSWEVERDRLGDRIVAMVAEHAPELPGLIAHRQVL
                     TPLDYEREYGLPEGSIYHGQMGLDQLASLRPIPGYGSYRSPVPGLFFCGAGAHPGGGV
                     SGAPGRNAARQILRQFRRLRRPAPL"
     gene            7662..7967
                     /locus_tag="VI520_05950"
     CDS             7662..7967
                     /locus_tag="VI520_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HLF37457.1"
                     /translation="MPSDKVRSDFDELSKIGQLFNAQSTAIAGVNRRIKSAQGTLEGG
                     DWIGEGAKKFFAEMEQQVNPSLKRLEQSLADASRKTQQIAQIMKQAEDEASSIFKLL"
     gene            8007..8300
                     /locus_tag="VI520_05955"
     CDS             8007..8300
                     /locus_tag="VI520_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37458.1"
                     /translation="MPDVRMNYDSMKKMEDAFSAAHKQLDDSMRAMQKLAKMMEDGAL
                     VGDGGTAYRGAIEGKLLKRMKALDAKMVELVKDIQGAVVATRDGVSTAQSRFK"
     gene            8332..>8582
                     /locus_tag="VI520_05960"
     CDS             8332..>8582
                     /locus_tag="VI520_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37459.1"
                     /translation="MSFLQFVRSEVSNVSSGVSQQQQIVSGVLDQIKGYIPKVQSAWI
                     GGDEKEFEADVMRKVVPAMMELIAAIGGVNLNLNKATSV"
BASE COUNT         1603 a         2583 c         2817 g         1579 t
ORIGIN      
        1 ggtactcggg agggcggact ataccacagc aggtacaatt cccgggctct ccaccggtct
       61 ccgccactgc cattgaagct ctacaacacg ctctccaggg agattcaaga gttcgctcct
      121 cagggcagcc agatcgggat ctacgtctgt ggcattaccc cctacgatac gacccatctt
      181 gggcacgcct tcacctattc ggtcgcggat gtcctgattc ggtatttgga ataccagggc
      241 ggggcggtcc gctacgttca aaacgtaact gacgtggatg acgacctgct gcgggtcgcc
      301 ggcgagcgcc aggaagattg gcgtcggatg ggtgaccgct ggaccgcgca ctacatcgaa
      361 gacatggcgg cactcaacat ccgtccacca gatgttctgc cgcgggcgac agacgtcatg
      421 ccgcagatta tcgagtcggt cgagcagctg ctacaggccg gggtggccta cgtggccggc
      481 ggcagcgtgt acttcgatct gaaggcttgg ccggcctacg gccgcttgtc gaggctcccg
      541 ccggatcaga tgcttccgat cgccaacgag cgcggaaacc ggccggaaga tccaaacaaa
      601 cggcagccct tggatttcgt gctttggcaa ccgcaggctc ctggtgagcc ggcctgggac
      661 agtccctggg gtccgggtcg ccccggatgg catatcgagt gttcgaccat ggcctcccag
      721 ctcatcggcc agaccgtgga tttgcacgct gggggtgagg acttggcgtt tcctcatcat
      781 gagagtgaga ttgcccagac tgagtccctg accgggaagc cgttcgccgg gcattggctg
      841 catgtcgccc cggtgcgcca cctcggagaa aagatcagca aatcgctcgg gaacctggtc
      901 atggtgcgcg acttgctgga tcggtggccg ccggatgcca ttcgggttta cctggcatct
      961 catcagtacc gccattcgtg ggcctacgac gccgagggcc tttacgatgc ggcccgggag
     1021 gctcaggccc tggcggccgc ggccgcactg gcaagcggcg agggtgattc gctatcggtt
     1081 gatgagctcg tctccgagtt tgcccaggcg atcgaacaag acctcaatac cccggcggcc
     1141 atcgccgtat tgcggaggct ggccgaatcg atcgtagcgg cttcgaacca ggggggtgcc
     1201 ttgggggcgg cccaggatgt gctgcgcaag ctgggtggtg tcctaggact gcggctggga
     1261 tccgccggtc cggaggggaa ggtcgtcgag ggctggcgcc ggcacctgac tcgattccgg
     1321 tccgagcccg ttccgggctg aagtcacagg cggaggaatc tcatgcaagg attgatcgga
     1381 caggagctcg gcgggtaccg catcatcgcc caaatcggga aggggggcat ggcgacggtc
     1441 ttcaaggcct accaaccctc cctcgaccga tatgtggcca tcaaggtgct gcccccctat
     1501 tacgccgagc aggacgagac cttcctcaag cgcttccgcc aggaggccaa ggccattgcc
     1561 agcctccggc accccaacat cctgatcgtc ctcgactatg gcgagctgga gcggacgacc
     1621 tatctggtca tggagttcgt ggaggcgggg accttgggcg gccagatggg gaagccgatg
     1681 gcgcccggcc agatggctgg gctcgtcggg caggtcgccg gggcccttca ttacgcccac
     1741 caggaaggcg tcgtccaccg ggacatcaaa cccagcaata tcctgctgcc aaagccaaac
     1801 tggccgctgc tgaccgactt cggtctggcc aagatcgttg gcgggactca attgactcag
     1861 tcggggacgg tcgccgggac gccggcctac atgtccccag agcagggacg gggcgagcgg
     1921 gtcgaccacc ggagcgacat ctactccctg ggaatcgtcc tgtacgaaat ggccaccgga
     1981 gtcgtccctt tccaagccga gaccccgatg gcagtgatcg tgaagcacat catcgacccg
     2041 ctcccgctac cgcgcagcaa gaccccggaa ttgtcggagg ccatcgagcg ggtgatcctg
     2101 aaagcgctgg ccaaagagcc ggccgatcga tttcaagatg tgggtcagct ggctgaggcg
     2161 ctgaccgcgg cggtcggtac attgccgccc gggaaagcga gtgttgcacc ctcgtttccc
     2221 tcccacgagt tgacgaccct gcaggtgact ggttcagggg ttgctgcgca gctcccggtt
     2281 gccacccgag tcgatccgga ggaggggatg gccgaggcag tggcggccgg tgcccgggcg
     2341 aaaggcgcga aatcttggtg gtttcgtctc gggttggcgg cgggtgttct ggtgctgggg
     2401 ttcgtcggct ttgccgccgc tcgggccctc ggcttcggag gtagcgcagg gagtccgatc
     2461 gccgacgagc cgggagaagc ccggacgctc gaacagctgg ttgcggacgg gtacgcgaac
     2521 ctacagtccg gcgatttcga ggccgcgatc cgcgacttcg agcgtgcgat cgagcgggaa
     2581 cctggaaatc cggaacgcta cttcgatgtg gctcgggctt acttcgcctc cggcgacccg
     2641 gatgcggcgt ccgatctgat tcaggaagca gtcgatctga tccctgggga ttccggtgtc
     2701 catgagtcgg ccggctgggt ctatcaggaa ttgggactgc atgaagaggc ggcgagtgag
     2761 tttcgcgccg cgctcgagct ggatccgcag gaactttggc tgtacgccgc catctcggat
     2821 tcgtatctcg cggccggact gcccgaggaa gcggcgggcg tcctcgcgga ggggctcgcc
     2881 aaccccgagg ttgccgctga ctccgccagc ctcgagtcca ttggctgggt attcctctct
     2941 ctaggcgatc ctgcccaggc agaacggacc ttcaatcagg cgatcaccgc cggcgcggac
     3001 ttcccgggac cctgggaagg gctggcggag gcggtgtatc agcagggcga gctggtcgac
     3061 gcactggcga tcctggccga cggcatgcaa tacttcccgg agcatgtgcc cttctaccaa
     3121 actaccggcg agctgctctg ggagcaaggc gagcttgaac aagcggcgac ctctttccag
     3181 caagcgatcg cgctcgatcc ggcaaacagc agcagctact ccagcttggc caggatgtac
     3241 agcgagatgg gcctgctgaa ggaggccgag caggtgttgc tgggagggct cgaggcacac
     3301 ccggagaaca caagctttta tggcgatctc gccagttatt actccggaca aggccgttac
     3361 gcggatgccg ttccactgta cgagacgctg actcaggccg aacccgacaa cggttggtgg
     3421 tttgcgtatt tggcggacac ataccgcaac ttgggggatc cggagaacgc gcggcgggtg
     3481 ctgggtgagg ctgccgaccg gagctcgggt gacggctggc taaacgactt catcggctgg
     3541 atttatgtcg ggctgggcga ttgtgacact gccctcggcc acttcgagta cgcgcttgag
     3601 ctcgactcca gcatcgagag ctcggcggag gggctgcagt cgtgtggggg ctagtgaccc
     3661 gctccgttcg ggcctaacga tcgagggagt ccagccacgg gcgccggatc gccgcggcgc
     3721 caactgcgca tagccaagtt tccaagtcgc caagcaaaca agtcgcgaag tactccagcc
     3781 gccaaatcac gaagtggcca agttgcccag tggcagggtc cttgcaacct gctcatgggc
     3841 caaatcaact cccgatgcgg gcttcaattg agtgtctccc aggacgagag atcccggccg
     3901 gacagcttct ccgggcggtg ctcggattgg gcctggcctt gggcgggtta ggcgggctag
     3961 ggctcgcact tctgctcggg atctacggat tcttccgggt atctgactcg atcgcgccgg
     4021 gagtagccgt cggccaattg ccagtcggcg gtttgctcat gacggatgcc gccgatcggt
     4081 tggatcaggc ttggaaccaa gatctggtgt tgaccgcggt ggacaccagc gaggccggcc
     4141 ggacctggca ggtttcgcct ggcgaattcg ggttgcgggt cgaggcggtt gagagcgccc
     4201 ggctggcgta cgcctatggg cgccaaggtg gtctgatccc gaatctcatc gacttagtga
     4261 caggcttcac aaacagttgg caaattgctc cggttgtcga gttcgatcct agtcaggcca
     4321 gaacggcact cacaacctgg gccgaaaaaa tcgccctgcc ggctgccgat gcgcgcctca
     4381 gactggaggc aggcgaagtg ctcatcacgc aggcccagtg gggaaggcgg ctcgacctgg
     4441 aggccagcct tgcgctgttg agtgccgacc cggggtccgt cctgctgcgc tatgggttca
     4501 ttccgctcgt cactgaaccg gttgcgccat tcattgcgga agtcgaggcg gccgccatcg
     4561 aggccgatca gctcctttcg gctgatatcg tcttgagagc gtacgacccg gtcaccgacg
     4621 agtggctgga ttgggctccg aatcgagcgg agatcggcgg ctggttgctg gtcggacagg
     4681 aggggggccg actgacggtg tcaatcaatg agcaggcggt tcgagctttc gtcggcggtc
     4741 tgccggtcgg cgatgaacgt actctggacg aagaggctgc cgcccagtcg ctcctggccg
     4801 gtctgcgcgg ggaggctggg gagccaatcc tgctccgcta cctgccgagg tactaccagg
     4861 tgtcgccgga ggacaccctg atttcgatca gcttcaaggt ggggatcccc tattggaagt
     4921 ggctggaaag caacccgcag gtggcggcca ggggactcgt tcccggagag tcgctgacca
     4981 tcccgccccg ggatgccatg ctcgaactgc cggtgattca aggcaagcga atagtcatca
     5041 gcatcagcga gcagcacatg tggctttatc aggacggaga attggttcgg gaaaacgtcg
     5101 tgagcaccgg catcccgaac tcaccgacga tgcctggcct cttccaggtc aagtctcact
     5161 tcctgaatgc gtacgcatcc aactgggatc tcaccatgcc gcacttcttg ggcatctacc
     5221 acgcggtgcc caacttcctc aacggcattc acggcttgcc gctgctctcg aatggggtgc
     5281 ggctgtgggc gaacgtgttg ggacggccgg cttcgtatgg ttgtgtgatc ctcgatttgg
     5341 aagccgccga gcacttgtac ggatgggccg aagacggggt ggtggtcgaa attcgcagct
     5401 aggctgctca gaactcgatc gtttctatct ccccactcgt gagatcgacc accgaatacg
     5461 acctggactc aaactgaagc ccgcccagcg cgcctgggtt gaacacccgg gtcgacccga
     5521 tggtttcgtc gcggcgctta tgagtgtgac cggtgaagac aaatcgatga atacccgatc
     5581 ggaccagccg attgagttcc ggcaggtcgt ctccatgagt cactgcgaac gatacatcgg
     5641 cgatctcgcc ttcgaaggtt ggcagggcga tgttgtgggg gtcgagctca cgcagggtac
     5701 gatagatctc tgcgggctca cggtccatgt tgccgtcaac atagatcact tgaaacccct
     5761 tcagctggcg cacgatctcg gggccgatca ggtccccgca gtggatcaat tggctgatcc
     5821 cccgccggcg gtagtcggcc agagcagtct cgaggttggc cacatcgtcg tggctgtctg
     5881 aaagcactcc aagtctcatc gatcgtcgaa ccttgccaga agtccttggt cggtcatcag
     5941 gctcaaagcg gggccggtcg gcggagccgc cggaattggc gcaagatctg ccgggcagca
     6001 tttcggcccg gggccccgct gacgcctccg cccgggtggg cgcccgcgcc gcagaagaaa
     6061 aggcctggaa ccggcgagcg gtagctgccg tatcccggaa ttggccgcag gctggcgagc
     6121 tggtctagcc ccatctgccc atgatagatg ctgccttccg gcaatccgta ttcccgttcg
     6181 tagtctagcg gggtgagtac ctggcgatgg gcgatcagcc caggcagttc gggggcatgc
     6241 tccgcgacca tcgccacaat ccggtcgccg agacgatccc gctccacctc ccagctcgag
     6301 tcgcgcaact gatagggagc atattgcatg gtgatggtca ggatgtgctt tccagacggg
     6361 gcccgcgagg ggtcgtacag ggtcgggatc gtcatgtcca gggtgggtct ttgcgagaac
     6421 tgcccgtatt tcgaatcgtc gaaggcccgt tccaggtagt ccaagtcggg gcagagcagg
     6481 agatgcccgg tcaggcccgc cgggtccttg atcgcgggga agtcggggag acgctccagc
     6541 gcgaggttca ctcgggccgt ggttccgcgg aagcggatgc ttcgaacccg tcttacgacc
     6601 cggacctcga gctgggaggg gccgaccaag tcgaacagag tgctgcgcgg atcggcgctc
     6661 gaggcgacca ccctggcccg gatctgcttt ccgttgcgca gctccactcc cactgcccgg
     6721 acgtcgtcga gcaggatgcg taccaccggc tctccggtga ggatttcggc cccgttggcc
     6781 cgggcggcgc gggccagcgc ctcagaaagc tgtcccgtcc cgccgagcac gaagctggcc
     6841 gtccgcggca gtcctggggc accgaggtac agcagcgtga acgccgtccc gggtgctttg
     6901 ggcccgaggg cgctgccggc gaccgctagc gagccgaggg cgcccttcag cggcccgctt
     6961 tcgaaccagt cgtccagcaa attctcggcc gagaggccga tcgtacgcag cagttccatc
     7021 atgcctgccc ggccgagcag gcggatgcca accgccactc gggcccagcc ccagagttcg
     7081 gagagcaaag ggggcagggc aggcggcgtg agcccgatcg cccgatccaa cccgctggcc
     7141 agcttgccaa actgccgcag gaagaccgga taacgctcgg cgtccgactt cgagatcttc
     7201 gccaattcgc gggaggcccg gtccggctgt ctccagaaga gaagggcagc atccggaccg
     7261 agcaatgaag cgcagcggat gggactctcg aacagctgaa ggccgtggtc cgcgagtctt
     7321 agttcgcgtt gcacctccgg gcgcagcatc ccggcggtct gggacccggt gtcgaaccgg
     7381 aagcttggga agacttcctc ggtcgcggcc gcgccgccca agatatccct ccgctccagc
     7441 acgaccaccc tgaggccgcc ccgggccaag taggcggcgc ttaccaggcc attgtggccg
     7501 cccccgatga tcacgacgtc gtattgtggg gtggttttgg gcatgacctc taggctatcc
     7561 ctgccgggac cgagcatctt gaaatcatcg gatactggcc ttagaatact gcagtctgtt
     7621 catccgattg gcccagcttc ggccaaccac aaggagtgtc tatgcccagt gacaaagttc
     7681 gctctgattt tgacgaattg tcgaagattg gtcaattgtt caacgcccaa agcactgcca
     7741 tcgccggtgt gaaccgccgg atcaagtccg cccaggggac tttggagggg ggggactgga
     7801 tcggcgaggg tgccaagaaa ttcttcgccg agatggagca gcaggtgaat ccatccttga
     7861 aaaggctgga gcagtcgctc gccgatgctt cccgcaagac ccagcagatc gcccagatca
     7921 tgaagcaggc cgaagacgaa gccagcagca ttttcaagct gctttagcca ggtccactga
     7981 tcacagatta ggccaggagg cccacaatgc ctgacgtaag aatgaactat gactcgatga
     8041 agaaaatgga agacgccttt tctgcggctc ataagcagtt ggacgattcc atgcgagcga
     8101 tgcagaagct ggccaagatg atggaagacg gcgcactggt cggcgacggc ggcacggcct
     8161 atcggggcgc cattgagggg aagttgctca agcgaatgaa ggccctcgac gccaagatgg
     8221 tagagcttgt aaaagacatc cagggtgcgg tggtcgctac ccgcgacgga gtttccactg
     8281 cccagagccg cttcaagtga atcgacttag cagccaataa ggagtcaacc aatgtcattc
     8341 ctgcagtttg ttcgctccga agtcagcaac gtatcgtccg gagttagcca gcagcagcag
     8401 atcgtctccg gggtcttgga ccagatcaaa ggctatattc ccaaggtcca gtcggcctgg
     8461 attggcggcg acgagaaaga attcgaagcc gatgtgatgc gcaaggtcgt tccagccatg
     8521 atggaactca tcgcggccat cggaggcgtc aatctgaacc tcaacaaggc caccagtgtc
     8581 at
//
LOCUS       DATDPQ010000263         7529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3519722, whole genome shotgun sequence.
ACCESSION   DATDPQ010000263 DATDPQ010000000
VERSION     DATDPQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519722"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2310
                     /locus_tag="VI520_05965"
     CDS             <1..2310
                     /locus_tag="VI520_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF37460.1"
                     /translation="IDIEALTDRIDQQLRASPKANIYGGGAGQIFITPRVKRVVDMAN
                     EEANRLNDEYISTEHIFLAILSERNTAISRLLNDEGITKERVYETVKEIRGGQRVTDR
                     KAESRYRTLEKYSRDLTTLAKEGKLDPVIGRDQEILRVIQILSRRTKSNPVLIGEAGV
                     GKTAIVEGLAQKIASNDIPEILMGKRVVSLDLGAMIAGSRFRGEFEERLKAAIEEIQR
                     AEGEIILMIDELHTVVGAGAAQGAMDASNMLKPALSRGELQCIGATTLDEYHKYIEKD
                     AALERRFAPVYVDEPTIDETIAMLQGLRDRYEAHHKVSFSDDALVSAARLSHRYVTGR
                     SLPDKAIDLMDEAAAKLRVALYSLPPDLKELKSALDRLMAEEEQAGLERDYERAAEKK
                     SERLRLEKEFHEKRDLWEAEHQLDEIVDVNDIAEVVHMWTGIPVSQMMETESEKLLRM
                     EDRLHERIVGQEEAIHALSDAIRRARAGLKDPRKPIGSFIFLGSSGVGKTEMAKALAQ
                     FMFDDEDALLRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDE
                     IEKAHPEVWNALLQILDDGRLTDGQGRVVDFRNTVLIMTSNLGNQFVTRAGTLGFRSR
                     SEDTQKTESEIKIEQALKDTFRPEFLNRIDEIIIFSQLTMEQMEQIVDLQMAKVRERL
                     AEHGLNVELSPAARSWLAEEGYDPAFGARPLQRALQKYIESPLSVKILRGEFQQGDTV
                     VVDHLADEGVVFRKPEGSTPVQIEEEVSA"
     gene            complement(2378..4093)
                     /gene="recN"
                     /locus_tag="VI520_05970"
     CDS             complement(2378..4093)
                     /gene="recN"
                     /locus_tag="VI520_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HLF37461.1"
                     /translation="MLTELRIRDFAIIGELELSFGPGFTVFTGETGAGKSIIIDAVEL
                     LLGGRADGTMLRSGAQAAIIEGAFQLDPQRLPEIGALLAQDDLLEDSGTVLLAREIRR
                     EGRNICRINGRTVGLTQLKAIGEWLVDVHGQSEHLSLLRVREHLQLLDRFAQTDSLLA
                     EYQTAFRRLQELRRRLTQLQSAAQERARRADLLRYQVNEIESAALREGEPAQLTAEAE
                     RLANAEKLAGLALNALAALDQTAGSEENSAIERLGEVAGALLHLGEIDPAMRGPSERA
                     QALAEELGELARLLGGYLEQVQPDPLRLDEVEARLGLISDLTRKYGVDLQAVQAYAAQ
                     AQAELDGILTADESLEALQAEQAQLVVRLAAVGHELSGRRRRAAEVLAEQVGAGMAEL
                     NMAGAQFAVDIQWQADPEGLPVEGKRVGFGPTGLDAVEFLVAPNAGEGLKPLAKIASG
                     GETSRLMLGLKSVLAQADRRPTLIFDEIDQGIGGRTGGVVGRKLWGLARDHQVLCVTH
                     LPQLAAHGDRHFKVEKSARSGRTLAEVKELQGAERLKELAEMLGDGSVANLHSAEALL
                     QNAAT"
     gene            complement(4103..4648)
                     /locus_tag="VI520_05975"
     CDS             complement(4103..4648)
                     /locus_tag="VI520_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HLF37462.1"
                     /translation="MTEALLTCYVHPDRPTKLRCNRCNRLICVSCAIQTPVGYRCREC
                     VRGQQQVFETAGRLDFALAGIVAALCAAVATPLLSRLGFWGFLFAPVVGGGIAEIVRA
                     AVRRRRSRRLPMVAGIGAAAGVLLYAGLRAAPFGLLLLGRAANLGAGALGGAGLSLLW
                     PLLYGGLMVGSLVARLQGIRL"
     gene            complement(4645..5535)
                     /locus_tag="VI520_05980"
     CDS             complement(4645..5535)
                     /locus_tag="VI520_05980"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HLF37463.1"
                     /translation="MSAGIPSTAGSAPPRSFVVAANPQLPEAVRLAEQINDVLNGRQL
                     ESSWGSLNDDSFRDRVEAHPVGLFIALGGDGTMLRAGQLCAPLDIPILGINLGHFGFL
                     TEVRKEDWLAALERVIQGDYWLEQRMMLQADHQRRDRRLGSWEALNECFIGRGEIVRP
                     VDLTTEIDERYLTTYVADGLIIATPTGSTAYALAAGGPILSPELRNILLVPVAPHLSV
                     ERPIVLDEGSTVRVAVRTTHQAILSVDGGLPIPLQSEDRVEIRAGKHVVRFARLQDPG
                     YFYRNLTSRMNQVAVLGSPE"
     gene            complement(5532..6185)
                     /locus_tag="VI520_05985"
     CDS             complement(5532..6185)
                     /locus_tag="VI520_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HLF37464.1"
                     /translation="MDRLEAIGERILSYFERKNGARDAALQRSRTLIRHSATAIRAAH
                     RQERELARQHLEQAKTIIDELRADLNSYPDLFHAGYTQDAFKEYAEASIVFALLVGDP
                     LPDPDELGVEYAAYLGGLGEAVGELRRRTLDILRQAHSAEAERLLEAMDEIYGLLVTV
                     DYPDAITGNLRRTTDLVRGLVERTRGDLTTSLQQDSLRAALDSVEKKLGPRAGDGEA"
     gene            complement(6191..6532)
                     /locus_tag="VI520_05990"
     CDS             complement(6191..6532)
                     /locus_tag="VI520_05990"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HLF37465.1"
                     /translation="MSEQPKSIAGQVQVHLPANLEPTYANFAMVTNSASEIVLDFAQV
                     MPRLPRAIVKARVVMTALNAKLLLKALSEHLQRYEAHFGEIKVPEGPHLSDQLFKSLP
                     PEKPPEKPPEE"
     gene            complement(6599..7003)
                     /gene="ssb"
                     /locus_tag="VI520_05995"
     CDS             complement(6599..7003)
                     /gene="ssb"
                     /locus_tag="VI520_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLF37466.1"
                     /translation="MFHKVILVGNLGRDPEMRYTPSGQAVTNLNVATNRTYTDGTGNQ
                     VKQTVWFRVSVWGKQAEASAQYLRKGRQVLVEGRLNPDDNGNPRIWNAQDGTPRASFE
                     VTAETVRFLGGGRDETATAEELGPTTEEEIPF"
     gene            complement(7157..>7529)
                     /locus_tag="VI520_06000"
     CDS             complement(7157..>7529)
                     /locus_tag="VI520_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37467.1"
                     /translation="APISANFALYEGLRMLAQEGLEARWARHRRCAEMFWEGLAALDL
                     TLLIPEPYRMVTVTTVRVPDGVNEAQVRGRLREEFNIEIAGGLGELKGKVWRVGLMGH
                     SAQEENVAALLGGLEAILHPA"
BASE COUNT         1449 a         2438 c         2320 g         1322 t
ORIGIN      
        1 atcgatatcg aagccctgac cgaccggatc gatcagcagc tgcgcgccag cccgaaggcc
       61 aacatctacg gcggcggagc aggccagatc ttcatcaccc cgcgagtcaa gcgggtggtg
      121 gacatggcca acgaggaagc caaccggctg aacgacgaat acatctcaac cgagcacatc
      181 ttcctggcca ttctctccga gcgcaacacc gccatctccc gtctgctcaa cgatgaagga
      241 attaccaaag agcgagtcta cgagaccgtc aaggagatcc ggggcggcca gcgggtaacc
      301 gaccgcaagg ccgagtcccg ctatcggact ctcgagaagt actcacggga tctcactacc
      361 ctcgccaaag aaggcaagct ggatccggtg attggacggg atcaagagat cctgcgggtc
      421 attcagatcc tgtcccgccg gacgaagagc aacccggttc tgatcggaga agccggagtg
      481 ggaaagaccg ccattgtgga aggcctggcc caaaagatcg ccagcaacga tatcccggag
      541 atcttgatgg gcaagcgggt cgtctcgctc gacctggggg cgatgatcgc cggctcccga
      601 tttcgggggg agttcgagga gcgcctgaag gccgccattg aagagattca acgggcggag
      661 ggcgagatca tcctgatgat cgacgaactg cacactgtcg tcggcgccgg cgcggctcag
      721 ggagcgatgg atgcttccaa catgctcaag cccgcgctgt cgcggggcga gctgcagtgt
      781 attggagcga ccaccctgga cgagtatcac aagtacatcg agaaggatgc ggcactagaa
      841 cgccgcttcg ccccggtgta cgtggacgag cccaccatcg acgagaccat cgccatgctg
      901 caggggctgc gcgaccgcta cgaggcccac cacaaagtga gcttctccga tgacgcgttg
      961 gtgtcagccg cccggctttc ccatcggtac gttacaggac gctccttacc ggacaaggcg
     1021 atcgacctta tggacgaggc ggccgccaag ctgagagtgg cactctactc tctgcccccc
     1081 gacctcaagg agctcaagag cgccctcgac cgcttaatgg ccgaggagga acaggccggg
     1141 ctggaacgcg actacgagcg ggcggccgag aagaaatctg aacgattgcg gctggagaag
     1201 gagttccatg agaagcggga cctctgggag gccgaacatc agctcgacga gatcgtggat
     1261 gtaaacgata tcgccgaagt ggtgcatatg tggaccggaa ttccagtcag ccagatgatg
     1321 gagaccgagt ccgagaaatt gctgcggatg gaagatcgac tgcatgaacg gatcgtaggc
     1381 caggaagagg cgatccatgc cctgtcggac gccattcgcc gggcgcgagc cggtctcaag
     1441 gacccccgca agccgatcgg atccttcatt ttcctgggtt cctcgggggt cgggaagacc
     1501 gagatggcca aggcgctggc ccaattcatg tttgacgacg aggacgccct gctgcgcatc
     1561 gatatgagtg aataccgcga gcagcacacc gtctcgcgcc tgttcggcgc accacccggc
     1621 tatgttggct acgaggaagg tggccagctc accgaggcgg ttcgccggcg accgtaccgg
     1681 gtgatcctat ttgatgagat cgagaaggcc cacccggaag tctggaacgc cctgctccaa
     1741 atcctggatg acggtcggtt gaccgatgga cagggacggg tggtggactt ccgcaacacg
     1801 gtgctgatca tgacctccaa cctgggtaat cagtttgtca cccgggccgg cactctcgga
     1861 ttccgcagcc ggagcgaaga tacccagaag accgagtcgg agataaagat cgagcaggcc
     1921 ctgaaggata cctttcggcc ggaattccta aatcggatcg acgagatcat catcttctcg
     1981 caactgacca tggagcaaat ggagcagatc gtcgacctac aaatggccaa ggtccgcgag
     2041 cgcttggccg agcacggctt gaacgtagag ctctcccccg cagcccgcag ctggctggca
     2101 gaggaaggct acgacccggc cttcggcgcc cgcccgcttc agcgcgccct ccagaaatac
     2161 atcgagagcc cgctctcggt gaagatcctg cggggggagt ttcagcaggg ggacaccgtg
     2221 gttgtggacc atctggcaga cgagggagtc gtcttccgca agccggaggg aagtaccccg
     2281 gttcagatcg aggaagaggt ttcggcttag gcggtcgagg gtagctcagc ttgagcctcc
     2341 gccgcggact ttgcgtcgga ggttcttttt tgtttagcta ggtcgccgcg ttctgcaaca
     2401 gcgcctccgc cgagtgcagg ttggccacgc tcccatcccc caacatctcg gccaattcct
     2461 tcaggcgttc ggcgccttgg agctctttga cttcggccag ggtgcgtcca gatcgggcgc
     2521 tcttttccac cttgaagtgc cggtctccat gggcagccag ctgcggcaag tgagtgacac
     2581 acaatacttg gtggtcgcgt gccaaacccc acagcttgcg ccccacgacc ccgccggttc
     2641 ggccgccgat tccctggtcg atttcgtcga agatcaaggt cggcctccgg tcggcctggg
     2701 ccaaaacact ctttaaccca agcatcaggc gggaggtttc gccgccggaa gcgatcttgg
     2761 ccagcggttt gagtccttcc ccggcattgg gagccaccag gaattccacc gcgtcgagcc
     2821 cggtcggacc gaagccaacc cgcttcccct caaccggcag gccctccggg tcggcttgcc
     2881 attggatgtc caccgcgaat tgggctccgg ccatattgag ctcagccatg ccggccccca
     2941 cctgctcggc caatacctcg gcggcgcggc gtctccggcc agagagctcg tggccgaccg
     3001 cggccaaccg aaccaccagc tgggcctgct cggcctggag ggcctcgagg ctctcatcgg
     3061 cagttaggat tccgtccaac tcggcctgcg cctgggccgc ataggcctgg actgcttgaa
     3121 ggtccacccc atactttcgg gtgagatcgc tgatcaatcc cagccgcgcc tcgacctcat
     3181 ccaaccgcag cggatcaggt tggacctgct cgagataacc acccagcagt cgagccagct
     3241 cgcctagctc ctcggcaagc gcttgggcgc gttcactggg ccctcgcatc gccgggtcga
     3301 tctcaccgag atggagcaac gcgcccgcga cctctcccaa ccgttcgata gcggagttct
     3361 cctccgatcc ggccgtctgg tccagcgccg ccaaagcgtt gagggctagc cccgccagtt
     3421 tttcggcgtt cgccaaacgc tcggcctccg cagtcagctg agcgggctcg ccttcccgga
     3481 gggcggccga ttcaatctcg ttgacctggt agcgcaagag gtccgcccgg cgcgcccgtt
     3541 cctgagccgc ggattgcaat tgggtcagcc gacgcctgag ctcctgcagt cgccggaatg
     3601 cggtctgata ctcggcgagc aagctgtcgg tttgggcgaa gcggtcaagc aactggagat
     3661 gctcgcggac ccgcagcagg ctcaggtgtt cggattgacc gtgcacatcg accagccact
     3721 cgccgatcgc cttgagttgg gtcagcccga ccgtccgccc attgatccga cagatatttc
     3781 ggccctctcg ccggatctca cgggccagga gcacagtgcc ggaatcttcc agcaagtcgt
     3841 cctgtgcgag cagcgctccg atttccggca atcgctgcgg atccagctga aaggcgcctt
     3901 cgattatggc ggcctgggcg ccggatcgaa gcatggtgcc gtcggcccgt ccgccaagca
     3961 gcagctcgac cgcgtcgatg atgatcgatt tgccagcccc ggtctcgccg gtgaacacag
     4021 tgaaacccgg cccgaagctg agctcaagct caccaatgat ggcaaaatcc cggatccgca
     4081 gctcagtcag catgagggcg gcttacagcc gaatcccttg caagcgggcc accaacgaac
     4141 caaccatcag acccccatag agcagcggcc acagcaggct cagcccggca ccacccaagg
     4201 cacccgcacc caggttggca gctcgtccaa gcagcaatag gccgaagggc gcggctcgca
     4261 ggccggcata aagcaagacc ccggccgccg cgccgatccc agccaccatt ggaagccggc
     4321 ggctgcgtcg tctccgaacg gcggctcgca cgatctcggc gatgccacct ccgaccaccg
     4381 gggcaaacaa gaagccccag aagcccagcc ggcttaacaa gggagtagcg accgctgcgc
     4441 acaatgcagc cacgattccg gcgagcgcaa aatcgagccg accggcagtt tcgaaaacct
     4501 gctgttggcc tcgaacgcac tcccggcagc ggtagcctac gggggtctgg atcgcacagc
     4561 tgacgcagat gagccggttg cagcgattgc agcgcaactt ggtgggccga tccgggtgga
     4621 catagcaggt gaggagcgcc tcggtcattc cggcgatcca agaaccgcga cctggttcat
     4681 ccgggaggtc aagttgcgat agaagtaacc cgggtcctga agccgcgcga accgcaccac
     4741 atgtttgccc gcccggattt ccacccggtc ctcactctgc aacggaatcg gcaagccccc
     4801 gtcgacgctc aggatggcct gatgggtcgt gcgaactgcc acccgcaccg tagaaccctc
     4861 atccaggacg atcggccgtt cgaccgacag atgcggggcg actggaacga gcaggatgtt
     4921 ccgcagctcc ggcgacaaga tcgggccacc ggccgccaag gcgtaggccg tcgagccggt
     4981 cggcgtggca atgatcagcc catcggccac gtaggtcgtc aaatagcgtt catcgatttc
     5041 ggtcgtcagg tcaaccggcc gaacgatctc gccacggccg ataaagcact cgttcaaagc
     5101 ctcccacgaa cccagccgcc ggtcccgccg ctggtgatcg gcctgcagca tcatgcgctg
     5161 ctccagccaa tagtcgccct gaatcactcg ctcgagcgcg gccagccaat cctcttttcg
     5221 cacctcggtc aggaatccga agtggcccag gttgattcca aggatcggga tgtctaaggg
     5281 ggcacacaat tgaccggccc gcagcatggt cccatctccg cccagggcga tgaacaggcc
     5341 caccggatgg gcttccaccc ggtcgcgaaa actgtcgtca ttgagcgatc cccaagaaga
     5401 ttccagctgg cgcccgttca ggacgtcgtt gatctgctcg gccagccgga ccgcctccgg
     5461 cagttgagga ttggccgcga ccacgaagct gcgcggcggg gccgacccgg ccgtggaagg
     5521 aattccggcg ctcaagcttc gccgtcccct gcccgggggc ccagcttctt ttcgaccgaa
     5581 tccagggcgg cccgcaggct gtcttgctgc aagctggtgg tcagatcgcc gcgagtgcgc
     5641 tccaccaggc cccgcaccag gtcggtagtc cgccgcaggt tcccggtgat cgcgtcggga
     5701 taatcaaccg tcaccagcag gccgtagatc tcgtccatgg cctctaacag ccgctcggcc
     5761 tcggcagaat gcgcttggcg caggatgtcc agagtccgcc ggcgcagctc gccgaccgcc
     5821 tcccccaatc cgccaaggta ggcggcgtac tcgacgccca actcatccgg atcgggaagc
     5881 gggtcgccga ccagcagtgc gaagacaatc gaagcctccg catactcctt gaatgcatcc
     5941 tgggtgtacc cggcatggaa caggtctggg tatgagttca ggtcagccct cagctcatcg
     6001 atgatcgttt tggcctgctc gagatgctgt cgggccagct cccgctcttg ccggtgggca
     6061 gcccggatgg cggtcgcgct gtggcggatg agagtgcgtg aacgctgcag ggctgcgtcc
     6121 cgggcgccgt tcttccgctc gaagtaggac agaatccgtt ccccgatcgc ctcgagtcgg
     6181 tccatgttgc ctattcctcg ggcggcttct cgggcggctt ctcgggcggc agactcttga
     6241 atagctggtc tgacaagtgg ggcccttccg gaaccttgat ctctccgaag tgggcttcgt
     6301 agcgttgcag gtgctcgctc aaagccttca acaggagctt ggcgttcaac gcggtcatca
     6361 ccactcgggc cttcacgatc gccctgggca gccgcggcat cacctgagcg aagtccagca
     6421 cgatttctga ggccgagttg gtaaccatcg caaagttggc ataggttggc tcgaggttgg
     6481 cgggcagatg cacctgaacc tggcccgcga tggatttggg ttgttcagac attgggctgc
     6541 tccatgagag ggccaggccc tcaaagacgg cttgagggcc tggcaatgac ttacgaccct
     6601 agaaggggat ctcttcttca gtggtgggac cgagctcttc ggcggtcgcg gtctcatccc
     6661 gccctccgcc gaggaagcgc acggtctcgg ccgtcacctc aaacgaggcg cgcggcgtgc
     6721 catcctgcgc attccagatg cgcgggttgc cgttgtcatc ggggttcagc cgaccttcga
     6781 ccagcacctg acgccctttg cgcaggtact gagcggaggc ctcggcctgc ttgccccaca
     6841 ccgagacccg gaaccagacg gtctgcttga cctggttgcc ggtaccgtcc gtgtaggtgc
     6901 ggttggtcgc cacgttcaga ttggtgacgg cctgaccgct gggcgtgtag cgcatctccg
     6961 gatcacgccc gaggttgcca acgaggatga ctttgtgaaa catggggctc cttttcgatg
     7021 gggggtcgtt tgccgttacg gcccgggtgg ccgattctgg attgagttac cccaccgtac
     7081 tagaacaatt gtactagtga ttgggtagtt tgtcaagcgg acttcccctg gtgggacgag
     7141 gccgcctggg ccgtcgtcag gccggatgca ggatcgcttc tagcccaccc agcaaggcag
     7201 ccacattctc ctcctgggcg gagtggccca tcaatccgac ccgccacacc ttgcccttca
     7261 actcgcccag cccaccggcg atctcaatat tgaactcttc ccgcaggcga ccgcggacct
     7321 gggcctcgtt cacgccgtcg gggactcgca cagtcgtcac ggtgaccatc cgatatggtt
     7381 ccgggattag caaggtgagg tcgagggctg ccagcccctc ccagaacatt tcggcgcagc
     7441 ggcggtggcg agcccagcgc gcctccaacc cttcttgggc cagcatacgt aacccttcgt
     7501 agagggcaaa gttagccgag atcggggcc
//
LOCUS       DATDPQ010000264         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2748306, whole genome shotgun sequence.
ACCESSION   DATDPQ010000264 DATDPQ010000000
VERSION     DATDPQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748306"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /gene="deoA"
                     /locus_tag="VI520_06005"
     CDS             complement(<1..236)
                     /gene="deoA"
                     /locus_tag="VI520_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HLF37468.1"
                     /translation="MRAVDVISKKRDGGELTKEEIEFFVAGFVRGEIPDYQASAWAMA
                     VLLRGMNARETTDLTLAMVASGDRLDLSQVVPLAV"
     gene            284..2266
                     /locus_tag="VI520_06010"
     CDS             284..2266
                     /locus_tag="VI520_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLF37469.1"
                     /translation="MPRADLVLTNAHVVTMDDRFQVFRDGALAILDGKLVAVGPAAEV
                     LAAYPGSERVDCGGKALIPGLINAHTHAAMSLLRGMADDRRLDVWLLGYVMPVEREFV
                     TPDFVRLGTSLACAEMIRSGVTCFADMYYFEETVAEAVAEAGMRAVCSQTVLKFPTPD
                     AESFEDALQRASEFIHRWRGHPLVVPSIAPHAAYTSTPEILRACTDLAVEFDVPLHIH
                     IAETAQEVQDWRKSYDMPVVPWVKKLGLFEAKVLAAHCVHVDLGEIHTLQHAGAGVSH
                     NPSSNLKLASGFAPVTQMLAEGINLGIGTDGPASNNDLDMFEELRLASFVAKITSGDP
                     TAAPARTTLAMATRLGARALHLGHLTGSLEAGKQADLALVDLDTLHSSPHFDHDPESI
                     YSRLVYTAKASDVTDVMVAGRWLMRDRELLTIRTDELLPAAAEIAKRIDAFLIEREDS
                     VLSKLVAIGGAEREESYEIQMKMRLPEPSLLQETLNSGRLEIVRHVHYREFDMYFTFS
                     DPRQGRLRYREDEFIDDKGEVYNVRSRLTLIGPAAEREYPQSVLLSRSRFIAPANHSE
                     RFYREYFIPSGEIAVHKDRLRWLVRFHGQEFFINIDQLLQPAVEGHFLEIKSRTWSRG
                     DAEVKAGLILELLETLGVEGVSEVRDEYPDLVTIAG"
     gene            complement(2253..>2541)
                     /locus_tag="VI520_06015"
     CDS             complement(2253..>2541)
                     /locus_tag="VI520_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLF37470.1"
                     /translation="VFLHKIIPGGADKSYGIHVAELAGLPRAVIHRAQEILMNLQADA
                     ARGRPAAPSQLTLFPDQNPLLEELERLQLEALSPLEALNLLYEWQGRFSPR"
BASE COUNT          470 a          810 c          781 g          480 t
ORIGIN      
        1 accgccagcg gcacgacctg cgagaggtcc agccggtcgc ccgaggcgac catcgccagg
       61 gtcaggtcgg tcgtctcgcg ggcgttcatc cctcgcagca gcaccgccat cgcccaggcg
      121 ctcgcctggt aatcggggat ctcacctcgg acgaacccag ccacgaagaa ctcgatctct
      181 tctttcgtga gctctccccc gtcacgcttc ttggaaatga cgtcaaccgc ccgcatggca
      241 tgagcatact ataggctcca cccgactgct ataatccagc cccatgccca gggccgatct
      301 ggtcctcacg aatgcgcacg tcgtcaccat ggacgaccgt ttccaggttt tccgggacgg
      361 cgcgttggcg atcttggacg ggaagctggt cgcggtgggg ccggccgccg aggttcttgc
      421 ggcctacccg ggctccgagc gggtcgactg cggcggcaag gccctcatcc ccggtttgat
      481 caatgcccac acccatgccg cgatgtcctt gctgcgcgga atggccgacg atcggcggct
      541 agatgtctgg ctattgggct acgtcatgcc ggtcgagcga gagttcgtca cacctgactt
      601 tgtgcgttta ggaacctcgc tggcctgcgc cgagatgatc cgcagcggcg tcacctgctt
      661 cgccgatatg tattacttcg aggaaaccgt ggccgaggcg gtagccgagg ccgggatgcg
      721 ggcggtctgc tcgcagacgg tgctgaaatt cccgacaccc gatgccgaat ccttcgaaga
      781 cgccctgcag cgggcctctg agttcatcca ccgttggcgg ggacatccgc tggttgtgcc
      841 ctcgatcgca ccccatgccg catacaccag cacaccggag atcctgcggg cctgcacgga
      901 tctggcggtc gaattcgacg ttccattgca catccacatc gcggagactg cccaggaagt
      961 gcaggattgg cggaagagct acgacatgcc ggtcgtgccg tgggtgaaga agctggggct
     1021 gttcgaggcc aaggtcctgg ccgcgcactg tgtccatgtg gacctgggcg agatccacac
     1081 cttgcagcat gccggggcgg gagtctctca taacccctcc agcaacctga aactcgcctc
     1141 cggttttgcc cccgtcactc agatgctggc ggagggcatc aatcttggca tcggcaccga
     1201 tggacccgct tccaataacg acctcgacat gttcgaggag ctgcggctgg ccagcttcgt
     1261 ggccaagatc acctctggcg acccgaccgc cgcccccgcc cggacgaccc tggcaatggc
     1321 gacccgcctg ggcgccagag ccctgcatct cggccacctg accggctcgc tggaggctgg
     1381 caagcaggcc gatctggcgc tggtcgacct ggatacactg cattcctctc cccacttcga
     1441 tcacgaccct gaatccattt actcacgact ggtttatacg gccaaagcga gtgacgtcac
     1501 cgacgtgatg gtcgctgggc gttggttgat gcgcgaccgg gagctgctaa ccatccgcac
     1561 cgacgagctg ctccccgccg cggccgaaat cgcaaagcgg atcgacgcat tcctgatcga
     1621 gcgcgaagac tcagttctct cgaagctggt ggcgattggc ggcgcggaac gggaagagag
     1681 ttacgaaatt caaatgaaga tgcgcctgcc ggagccgagc ctgctccagg agaccttgaa
     1741 ttcgggtcga ttggagattg tgcgtcacgt ccactatcgc gagttcgaca tgtacttcac
     1801 cttttctgat ccgcggcagg gaaggctgcg ctaccgagaa gacgagttca tcgacgacaa
     1861 gggagaggtc tacaacgtgc gctcccgact gaccttgatc gggccggctg ccgagcggga
     1921 gtatccgcaa tcggttctgc tatcccgctc ccgcttcatc gccccggcta atcacagcga
     1981 gcgattctat cgggagtatt tcattcccag cggcgaaatt gccgtgcaca aggatcggct
     2041 gcgctggttg gtccggtttc acggccagga gttcttcatt aacatcgatc agctgctgca
     2101 gccggcggtc gagggccact tcctggagat caagagccgg acctggtcgc gcggcgatgc
     2161 cgaggtcaag gccggattga tcttggaatt gctggagacg ctgggtgtcg aaggagtatc
     2221 cgaggtccga gatgagtacc cagacctggt gactatcgcg ggctgaagcg accctgccac
     2281 tcgtagagca ggttcagcgc ctccaacggg ctgagcgcct ccagctggag gcgttccagc
     2341 tcctccagca gggggttctg gtcaggaaaa agcgtcaact gactgggagc ggccggtctc
     2401 ccccgggcgg catcggcctg caagttcatc aagatttcct gggcccgatg gatgacggcc
     2461 cgcggcagcc cagccaattc ggcgacatgg atgccatagg acttatctgc cccacccgga
     2521 atgatcttgt gcaggaagac c
//
LOCUS       DATDPQ010000265         6344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_384882, whole genome shotgun sequence.
ACCESSION   DATDPQ010000265 DATDPQ010000000
VERSION     DATDPQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384882"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VI520_06020"
     CDS             complement(<1..827)
                     /locus_tag="VI520_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLF37471.1"
                     /translation="MADQPAELDRNLRVHDDIMGTIGNTPLVRLSRVTSGLRCRVFAK
                     LEFFNPGGSVKDRIAPRILEAYERSGDLRPGGTVVEATSGNTGVGLAIACALKGYQAI
                     FVMPDKMSQEKIQLLRAFGARVVIAPTAVAPQDPRSYYSVARRLVAETPNSVLADQYH
                     NPVNPESHYLTTAPEIWHQTGGTVTDIVVGMGTGGTITGIAQYMREEGRKVRIVGVDP
                     IGSLLYDAWRAGGSAVGLEAITYKVEGIGEDFVPSTLDLGLVDEVVQVDDAEAFLWTR
                     "
     gene            962..2335
                     /locus_tag="VI520_06025"
     CDS             962..2335
                     /locus_tag="VI520_06025"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HLF37472.1"
                     /translation="MQEPLPALDFARQHRGAALEDLKEFLRIPSISTLPEHKPDLLRA
                     SNWVADQLRQLGLEPVEVLPTAGHPVVYGEWLKSGDSAPTVLVYGHYDVQPVDPLKLW
                     ESPPFEPAIRGEDIFARGASDMKGQIVAQLKAAEALVNTSGLPLNLKFMIEGEEEIGS
                     PNLGAFIEQQRSKLACDFCLNLDAGVLGPDVPSLTLALRGLAYFEVRIQGPAGDLHSG
                     TFGGAVENPANVLARLIAGMHDAAGRVALPGFYDQVRPMTERDRADLAQLPQGEAWWM
                     EQSATKALGGEAGFTATERATSRPTLDVNGMLSGFTGAGSKTVLPAKAMAKISTRLVP
                     DQRPEQVHKSLIEYMRRSAPPTVDWEVEILAGAIPGIIDRDAPEVQTAAKALEAAWGK
                     PAVYKREGGTVPVVGMIQELLGVNSLLLGFGLPDDNLHAPNEKFHLPNFYRGIDTLIR
                     FLAALPA"
     gene            2338..3276
                     /locus_tag="VI520_06030"
     CDS             2338..3276
                     /locus_tag="VI520_06030"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HLF37473.1"
                     /translation="MGADSTRRSTYGGQAVIEGVMMRGQRAYSIAVRAPNQSIVVESW
                     PLGRLARSRLRPIPFLRGPLVLWEALSLGVRSLAFSANAQALKQSERIEGASLTLSLA
                     GSLLVGTAGFLLLPAGAAHVLQGWLGFSAFTANIVEGGLRLALLIGYIWSIGRIPEIR
                     RVFAYHAAEHMTIHAFEAGDELRPARVARHPKEHPRCGTAFLLTLALLSVVLFAVLGP
                     MSLGARLATRLLAIPLLASIAYEYLRLTARLAETWLGRILIWPNLALQHLTTRPPDQG
                     MLEVAIRGFEALLERERELEQELEGAQASSAPGAHA"
     gene            complement(3273..5864)
                     /locus_tag="VI520_06035"
     CDS             complement(3273..5864)
                     /locus_tag="VI520_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37474.1"
                     /translation="MAESNHSQLSIAERILSSRARLVGHDDSPRSLRKAEAEGGCGVI
                     GIASSVPIEGRHLLQALVQMRNRANGKGGGAALAGIDPAALGTSSQVLANCYLLAVAY
                     LAEGARSEVERERIDSMFEVEHIHLLPVLASLDPPAPRVVLYFVRPKSSRLDELADLG
                     KLRGINRPTLEDEFVYQNSYKLNSSYYASLGEKRAFVLSHGKDLLVLKLVGFGEDVFH
                     AYGLEGLRAHVWIGHHRYPTKGRVWHPGGAHPFVGLHEALVHNGDFANYASVSAYLAQ
                     NSIRPLFLTDTEVSVLVFDLLHRTFGYPLEYVIEALAPTTERDFAQLPERRRRIYQWI
                     QAAHMHGSPDGPWFFLLAQTAANQNGGREARLIGITDTSMLRPQVFALQQGGVGQPSI
                     GFAASEKQAIDAALQSLAGSDSRFGTRADLVWNARGGSHTDGGAFVFSVQREPNGEAE
                     LRCQDKFGRQIQVSETGFPYRGGVTIRPTEKSPIELAGSAGTLFQAATQALPGWGYPQ
                     LQAFLAELTGGPLDLGDQLELLSRLLDRRYPTGSMRRSSVLSLVDQALANWARRVAES
                     AGNGFVHHEKGNPLPQARGEAGSVVVQAKGYPAQGRGSLALEVRELVDRGFSRIILMG
                     ASGQRFIGAGLGSATEGVQIEVFGSSGDYLASGIDGAELVVHGSAQDQLGQIMKLGRL
                     VVHGDVGQTFMYGAKGGEAFVMGSAAGRPLINAVGKPRVVINGTCLDYLAESFMAGDP
                     LDGGGFVVLNGVGFDPVGKVVELDSPYPGGNLFSLASGGAIFVRDPDRQLTEDQLNGG
                     RFADFRSEYWELVRPLLEVNERLFGISIERLLGHQGRAAAPAAVYRAILPSGHKALQP
                     EQAWVAH"
     gene            complement(5868..>6344)
                     /locus_tag="VI520_06040"
     CDS             complement(5868..>6344)
                     /locus_tag="VI520_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HLF37475.1"
                     /translation="LQVDGSYGGTGAAPEIAKKNIAMPVEFAIPKVHRYLLAEGVRDE
                     IVVLASGGLRTADDIAKAIALGADGAVLGTAELVAIGCTRCKNCERGRGCPFGLTTTD
                     PELSLLIDPAWGAERISNLYGAFQSRLREILRQSGLSRVRELRGRTDLLRYSPRPE"
BASE COUNT         1180 a         2054 c         1927 g         1183 t
ORIGIN      
        1 cgggtccaca ggaatgcctc ggcgtcgtcc acctgaacta cctcgtcgac cagccccaag
       61 tccaaggtag agggaacaaa atcctcgcct atcccttcga ctttgtatgt gattgcttcc
      121 aacccgaccg cactcccgcc cgcccgccac gcgtcgtaga gcagcgagcc gatcggatcg
      181 actccgacga tccgaacctt gcgtccctcc tcgcgcatat attgggctat cccggtgatc
      241 gtacctccgg ttcccatccc caccacgata tcggtgacag ttcccccggt ttgatgccaa
      301 atctccgggg cggttgtcag gtagtggctc tcagggttga ccggattgtg gtattggtcg
      361 gccaggaccg aattcggagt ttctgccacc agccgccggg ccaccgagta gtaggagcga
      421 ggatcttggg gcgccactgc ggtcggggcg atcaccaccc gcgctccaaa cgcccgcagc
      481 agctgaatct tctcttggct catcttgtcc ggcatgacaa agatggcttg atagcccttc
      541 aatgcgcagg caatcgctag cccgacaccg gtattgcccg atgtggcttc gaccaccgtg
      601 ccgcccggcc gcaggtctcc ggatcgctcg taggcctcca ggatccgcgg cgcaatccgg
      661 tcttttaccg aaccccctgg attgaagaac tcgagtttgg cgaaaactcg gcagcgcagg
      721 ccgctggtga ctcgagaaag ccggaccaaa ggcgtgttgc cgatggttcc catgatgtca
      781 tcgtgcaccc gcaaattgcg gtcgagctca gcgggctggt cagccatggc gcgcctcggt
      841 cggattcaag cgtaccatag ctgggaatcg gcggcgaccg gcgtttccag gttacaatct
      901 cggcgaacgc tagagtcgct gcgcgttccc cgtgagcgta tcaccaaccc atggaggtca
      961 gatgcaggaa ccgctacccg ccctggactt tgcacgccaa caccgtggcg cggccctgga
     1021 ggatctgaaa gaatttctgc gaatcccatc gatctcgacc ctgcctgagc acaaacccga
     1081 tctgctgcgg gcgtcaaact gggtggcaga ccaattgagg caactagggc tagagccggt
     1141 tgaggtgctt ccaacggcag ggcatccagt cgtgtacggg gaatggctga agtccggcga
     1201 ttccgctccg acggttctgg tttatggcca ctatgatgtc cagccggtag acccactcaa
     1261 gctctgggag agtcccccgt tcgaaccggc aatccgcggc gaggatatct tcgccagggg
     1321 cgcctccgac atgaaggggc agatcgttgc acagctcaag gccgcggagg cgctcgtcaa
     1381 cacctctggc ctcccgctca acctgaaatt catgatcgag ggcgaggagg agatcggctc
     1441 tccgaatctg ggagccttca ttgagcagca gcggagcaag cttgcctgcg acttttgcct
     1501 caatctcgac gcgggcgtgc tcggaccgga cgttccgtct ttgaccttgg ccctccgagg
     1561 gttggcatac ttcgaggttc ggatccaggg gccggcgggt gacctgcatt ccggaacctt
     1621 tggcggcgcg gtggagaatc cggccaacgt gctggcccgg ctgattgccg gaatgcacga
     1681 cgcggcgggg cgggtggccc tccccggctt ctacgatcaa gttcggccaa tgacagaacg
     1741 cgatcgggcc gatctggccc aactgccaca aggtgaggcc tggtggatgg agcaatcggc
     1801 gaccaaggcg ctcggcgggg aggcgggatt cacagccacc gagcgggcaa cctcccgccc
     1861 gacgctcgac gtcaacggga tgctcagcgg attcacgggc gccggttcga agaccgtgct
     1921 cccggccaag gccatggcga agatctcaac ccggctcgtg ccggaccagc ggcccgagca
     1981 ggtccataaa tctctgatcg agtacatgcg acggtccgcg cctccgactg tggactggga
     2041 agtcgaaatt ctggcggggg ctatccctgg gatcatcgat cgcgacgctc cggaggttca
     2101 aaccgcggcc aaagcgcttg aggcggcatg ggggaaaccg gcggtgtaca aacgagaggg
     2161 tggcactgtc ccggtcgtcg ggatgatcca ggaattgctc ggagttaaca gcttgctgct
     2221 ggggttcggc ttaccggatg acaacctgca tgccccgaac gaaaaattcc acctccccaa
     2281 tttctaccgc ggcatcgata ccctgatccg gttcttagcc gcccttcccg cctgagaatg
     2341 ggcgccgact cgacccgccg atcgacctac gggggccagg cggtgatcga aggggtgatg
     2401 atgcgcggcc aacgggcata cagcattgcc gttcgggctc ccaatcaatc cattgtggtg
     2461 gagtcatggc ctctcggccg gctggctcgc agccgcttga gacccattcc cttcctgcgc
     2521 gggccgctgg tcctgtggga ggcgttgtcg ttaggggttc gctccctcgc attctccgca
     2581 aacgctcagg cccttaagca atccgagcgg atcgagggcg cctcgctgac cctctcgctg
     2641 gccggctcct tgttggttgg caccgctggt ttcttgttgc taccggccgg ggcggcccac
     2701 gtactgcagg ggtggctcgg attcagtgca ttcacggcca acattgtcga gggaggcctt
     2761 cggctggctc tgttgattgg ctacatctgg agcattggtc ggattccaga gatccggcgg
     2821 gtgttcgcct accacgccgc agaacacatg accattcacg ccttcgaggc aggcgacgaa
     2881 cttaggccag cgcgggtcgc ccggcatccc aaggagcatc ctcgctgcgg aacggctttc
     2941 ctcttgacgc tggcgttgct ctcggtggtg ttgtttgcgg tgcttggccc gatgtcgctc
     3001 ggcgctcggc tcgccacccg gctgctggct atcccgctgc ttgcgtcgat tgcctacgaa
     3061 tacctgcgtc tcacagcccg gctggcagag acttggcttg ggaggatctt gatttggccc
     3121 aacctggcct tgcagcacct gacaacccgc ccgccagatc agggcatgct ggaggtggct
     3181 attcgtggct tcgaggcgct gctcgagcgg gaacgcgagc tagagcagga gctcgagggt
     3241 gctcaggcaa gctcggcccc gggcgctcac gcctagtggg caacccaggc ctgctcgggc
     3301 tggagcgcct tgtggccaga aggcagtatc gcccggtaaa ctgccgctgg agccgctgcc
     3361 cggccctggt gcccgagcag gcgttcaatt gagatcccga agagccgttc gttcacctcc
     3421 aacagcgggc ggaccagctc ccagtattca gaccggaagt ctgcaaaccg gccaccgttc
     3481 agttgatcct ccgtgagctg tcgatccggg tcgcggacaa agatcgcccc accggaggcc
     3541 aaggaaaaca ggttaccacc tggatagggc gagtcgagct cgaccacctt acccaccggg
     3601 tcgaaaccca ccccattcag cacaacgaac cctccgccat ccagcgggtc tccggccatg
     3661 aaggattcgg ccaggtaatc caggcaggtc ccgttgatga cgacccgtgg cttcccgacg
     3721 gcgttgatca gggggcgccc ggcggcactc cccatcacga aggcctcccc tcctttggcg
     3781 ccgtacatga aggtctggcc gacgtcacca tgaacgacca gtcggcctag tttcataatc
     3841 tgccccagct gatcctgggc ggagccgtgc accaccagct cggctccgtc gatcccggat
     3901 gccagataat ctcccgaaga tccgaagacc tctatctgga ccccctcggt tgcggagccc
     3961 agtccggcgc cgataaaccg ctggccgctc gctcccatca gaataatccg ggaaaagccg
     4021 cggtcgacga gctctcgaac ttccagcgcc aacgaacctc tgccctgagc cgggtaacct
     4081 ttcgcctgca ccacaactga cccggcctct ccgcgagcct ggggcagcgg gttccccttc
     4141 tcgtgatgca cgaacccgtt gcccgcggat tcggcgaccc tgcgagccca attcgccaag
     4201 gcctgatcga ctagcgagag gaccgagctc cggcgcatgg atccggtggg atatcggcga
     4261 tcaagcagcc ggctgagcag ttccaattgg tcgccgagat cgagagggcc gccagtcagc
     4321 tcggcgagga acgcctgcag ctgcggatag ccccaacctg gcagggcctg ggtcgccgcc
     4381 tggaagagcg tgcctgctga tcctgccagc tcaatgggag acttctcagt tggccggatc
     4441 gtgaccccgc cccgataggg aaatccggtc tccgaaactt ggatttgccg gccgaacttg
     4501 tcttggcagc gcagttcggc ttcgccgttc ggttcccgct ggacgctgaa cacgaacgct
     4561 ccgccgtcag tgtggctgcc gccgcgggca ttccatacca agtctgctcg agtcccgaat
     4621 ctggagtcgg aaccggccag gctctggagc gccgcatcaa tggcctgttt ctcggaggcg
     4681 gcgaatccga tcgaaggctg gccgaccccg ccctgctgga gcgcgaagac ttggggccgc
     4741 agcatgctgg tgtccgtgat cccgatcaaa cgggcttccc gcccgccgtt ctggtttgcg
     4801 gccgtctgag caagcaagaa gaaccacggc ccatccggtg aaccatgcat atgggcagcc
     4861 tgaatccact gatagattcg gcgccgtcgc tcgggcaact gtgcgaagtc tcgctcggtg
     4921 gtgggcgcca gggcctcgat cacgtactca agcggatagc cgaaggtgcg gtgcagcaag
     4981 tcgaagacca ataccgagac ctcggtatcg gtcaagaaca aaggacggat gctgttctgc
     5041 gccaggtagg ctgacaccga agcatagttt gcgaagtctc cgttgtgtac cagcgcttcg
     5101 tgcagtccca caaatggatg agctccgccc ggatgccata cccgaccctt ggtcggatag
     5161 cggtggtgac cgatccacac gtgagcgcgc aggccttcta gtccataggc gtgaaacacg
     5221 tcttcgccaa atccaaccag tttcagcacc aggagatcct tgccgtgcga taacacaaac
     5281 gctcgcttct cacccagcga ggcatagtaa ctgctattga gcttgtagct attctggtaa
     5341 acgaactcat cttccagagt cgggcgattg attccccgca gctttccgag atcggccagc
     5401 tcgtccagcc gactactttt cggccgcaca aaataaagca cgaccctggg agccggcgga
     5461 tcgaggctgg caagcaccgg gagcaagtgg atgtgctcca cctcaaacat cgaatcgatc
     5521 cgctcccgtt caacttcgga acgagcgccc tcggcaagat aggccaccgc cagcagatag
     5581 cagttggcaa ggacttggga actggttcct aatgcggccg gatcgattcc tgccagcgcc
     5641 gcgccgccgc cctttccatt ggcgcggttg cgcatctgaa ctaaggcttg aagcaaatgg
     5701 cggccttcga ttggcacgct gcttgcgatt ccgatgacgc cgcagcctcc ctcggcctcc
     5761 gccttgcgca gggagcgcgg cgagtcgtcg tggcccacca gccgggcacg ggaggacaag
     5821 attcgttccg caatcgagag ctgggaatga ttggactctg ccatcgctta ctccggtcgc
     5881 ggtgaatagc gaagcaaatc agttcgaccg cgcagctcgc gaacccggct cagccccgat
     5941 tgcctcaaga tctcgcgcag gcgggattga aacgccccat acagattact aatccgctct
     6001 gcgccccagg ccggatcgat gagcagggaa agctcgggat cggtagtggt caggccgaat
     6061 gggcatcctc ggccgcgttc gcagttcttg cagcgggtgc agccgatggc caccaattcc
     6121 gcggtaccca acaccgcccc atccgcaccg agggcgatcg ctttggcgat gtcgtcggcg
     6181 gttcgcaatc cgccgctcgc cagcacgacg atctcgtccc gcacgccctc cgccaacagg
     6241 taacgatgca ccttggggat cgcgaactcc accggcatcg cgatgttctt cttggcgatc
     6301 tccggggcgg ctccggtgcc cccgtacgac ccgtcaactt gcag
//
LOCUS       DATDPQ010000266         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2230678, whole genome shotgun sequence.
ACCESSION   DATDPQ010000266 DATDPQ010000000
VERSION     DATDPQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2230678"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2349)
                     /locus_tag="VI520_06045"
     CDS             complement(<1..2349)
                     /locus_tag="VI520_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLF37476.1"
                     /translation="MADKGLKNYSRIRYTFPLPSLIEVQLDSFGWLKVEGLADLFDEV
                     SPIESYNGGMKLYFPGKTQESKQFGLKYWFEETKHDLEECVERDLTYAAPLYVSVLLA
                     GTEVPEPIKQDIFLGDFPIMTEKGTFVINGTERVVVSQLIRSPGVYFEAPEDRTTGRR
                     LASAKLIPDRGAWMEFESRKSNYLTLKFNRKRTIPVTILLRALAAINDGTKKPAIKDG
                     SDEELLQLFAQADVDPDHPYIATTMRQEPTWERRDNRSVAEEALVEFYRRMRPGDPPT
                     LDNAREYLRDQLIDPRRYDLERVGRYKLNGKLGLEKLVPEDHRQVTSWDVVRIVERMI
                     RINNGAADPDDIDHLGNRRVKTVGELIQSKLRVGLRRMERVILERMSIRDQEQLSPVS
                     LINIRPVVAAIREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRD
                     VHHSHYGRICPIETPEGPNIGLIGRLASYARVNRFGFIETPYRRVVNELSSKDKRLVG
                     KRLRGSWKDAKGEKVLAKDGTVVSNSLASGMARRKALIPVVPFVSDDTQYLSADEEDK
                     YVIAQANTPLNDRMEFSNPRASCRRHGDFFFATPDQINFMDVAPRQIVGVSASLIPFL
                     EHDDANRALMGSNMQTQAVPLIDPEVPLVSTGMERQAAYDSGQVILAEESGEVESVSG
                     STVVVRSKGASRTYKLRKFNRSNQSTCIDQRPSVIKGQRVTKGDVIADSTSTEGGELA
                     LGQNVLCAFLSWEGGDFEDAILISERLVQQDRYTSVHIEKHEV"
     gene            complement(2565..>4761)
                     /locus_tag="VI520_06050"
     CDS             complement(2565..>4761)
                     /locus_tag="VI520_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLF37477.1"
                     /translation="GDWDQAYVEFSRRLEQAGGPEEIAEALYGRGEALLQSGLYAGAQ
                     VEFSELIERFPTSERSAQARLLRAQALLGLGQLELSAMDYAAYLEMRPGVLDAYVQER
                     LGDALRLAGKPLEAVGHYQNAIAAPRITDVLPLLVKIGRCYLEASELELALTQFDSIS
                     AQTTDPGTRASMNLLAGETLEGLGDFEGAVSRYMDSVSNYPTAYDSYIALIRLVEAGI
                     PVDEFQRGLVDYQAGAYEPAVAAFDRVLGSVPSAAGFYYRGLSHLALGNSQQALADFE
                     WVVTNFPDDALWPDSALAKARTQWAYLDQYSAAVDTYLALVATRPEPEIGSEALFAAG
                     RTAERGADLQRAAEIWLRLPELYPGQDLTHQAAFLAGVAWFRLGRFAEADLAFKSALA
                     NAITSADAAAAELWIGKTQQAGGNLQAAEAAWNRAVEADPTGYYSVRAAELLDGSPVF
                     EGSGAFDFAYDLETERSEAEQWLRQTFSLLGPDPLSEPNLAITGDQRYLRGRELWELG
                     ELELAAAEFSSLRQSVESDPEATYRLMHELLGLGLYREAIFSSRQILRLAGMDNAATL
                     DAPVYFNRIRFGPYFGELILPEAARYGLDGLFLLSVVRQESLFEGFATSSAAAHGLMQ
                     VIPSTGESIAAQLGWPPDYTSADLYRPIVSVRFGTFYLATQRDRFEGDLFAALAAYNA
                     GPGNAQFWKELAPSDPDLFLEVIRLDQPQLYIRVIFEVYEIYRNLYSSG"
BASE COUNT          915 a         1487 c         1400 g          959 t
ORIGIN      
        1 aacctcgtgc ttctcaatgt gtaccgaggt gtagcgatcc tgttgtacca gccgctcgga
       61 aatcagaatc gcgtcttcga agtcgccgcc ttcccaagag agaaaggcac agagcacgtt
      121 ctgccccaaa gccagttcac cgccttcggt ggacgtcgaa tccgcaatga catccccctt
      181 ggttaccctt tgacccttga tcaccgatgg gcgctggtcg atgcaggtgc tctgattgga
      241 acgattgaac ttgcgcagct tgtaggtccg gcttgcgcct ttggaccgga caaccaccgt
      301 actgcccgag accgattcga cctccccact ctcctcggcc aggatgacct ggcccgaatc
      361 gtaggctgcc tggcgttcca ttccggtgga aaccaaaggc acttcagggt cgatcaaggg
      421 taccgcctgg gtctgcatgt tggagcccat caaggcccgg tttgcatcat catgctccag
      481 gaacggaatc aaggaagcgc ttacccccac gatctggcgc ggagccacgt ccatgaagtt
      541 gatctggtct ggggtggcga agaagaaatc accgtgtcgg cggcaggagg ctcgcggatt
      601 ggaaaactcc atccggtcat tcagcggggt gtttgcctga gcgattacgt atttgtcctc
      661 ttcatcggcg gacaaatact gggtgtcatc ggagacgaag ggaaccaccg ggatcaatgc
      721 tttgcgtcgg gccatcccgc tcgctaggct gttgctgacc acggtcccgt ctttggccaa
      781 aaccttttcc cccttggcat ccttccagct accccgcaat cgctttccta ccaaccgctt
      841 gtcctttgaa ctcaactcgt tcaccacccg acgatagggg gtctcaatga aaccgaaccg
      901 attcacccgg gcgtaggaag ccagccttcc gatcaggccg atgttggggc cttccggggt
      961 ctcaatcgga caaatccggc cgtagtgaga atggtggaca tcgcgcacgt cgaagccggc
     1021 ccgttcccgc cgcaggccgc ccggccccaa agcagacaag gtgcgcttgt gtcttagctc
     1081 ggccagcgga ttggtctgat ccatgaattg ggatagctgg ctggagccga agaactctcg
     1141 gatcgcggct acaaccgggc ggatgttgat caacgagacc ggtgagagtt gctcctgatc
     1201 ccggatcgac attcgctcga gaatgacccg ctccatcctg cgcaaaccaa cccgcagctt
     1261 gctctggatt agctcgccaa ccgtctttac ccgccgattg ccgaggtggt cgatgtcatc
     1321 cgggtcggcg gccccgttgt tgattcggat cattcgctca acaattcgga ctacatccca
     1381 actcgtaacc tggcgatggt cctcgggcac cagtttctcc aacccgagtt tcccattcag
     1441 cttgtagcgg ccgacccgct ccaggtcata tcgccgtggg tcgatcaatt ggtcccgcaa
     1501 atactctcgg gcgttgtcga gggtcggtgg gtcgcctggt cgcattcgac ggtaaaactc
     1561 gaccagtgcc tcttcggcca cgctgcggtt gtcgcgccgc tcccaagtcg gctcctgccg
     1621 catggtagtg gctatatagg ggtggtcggg gtcgacgtcc gcctgggcaa agagctggag
     1681 cagctcttcg tccgatccgt ccttgatcgc cggcttcttg gtcccgtcgt tgatcgccgc
     1741 cagcgccctt agcaggatcg tgaccgggat ggtccgcttg cggttgaact tcaaagtcag
     1801 gtagttactc ttgcggctct caaactccat ccaagcgccc cggtcgggga tcagcttggc
     1861 cgaggccaat cgccggccgg tggtccgatc ctcgggagcc tcaaaataga caccggggga
     1921 gcggatcaat tgcgagacga ccacccgctc ggtgccattg ataacgaatg tgcccttctc
     1981 ggtcatgatt gggaagtcgc ccaggaaaat gtcctgcttg atcggctcgg gcacttcggt
     2041 gccggcaagc agtaccgaga catagagcgg ggcggcgtaa gtcagatcgc gttcgacgca
     2101 ctcttccagg tcgtgcttgg tctcttcaaa ccaatacttg agtccgaact gtttggactc
     2161 ctgggtcttg cctgggaaat agagcttcat ccccccgttg tacgattcga tcggcgagac
     2221 ctcatcgaac aagtcagcaa gtccctcaac tttgagccat ccgaacgaat cgagttgcac
     2281 ttctattaat gaaggcagtg ggaaggtgta gcgaatgcgc gagtaattct tgaggccttt
     2341 gtcagccata ccgctccctt tcgtccgtgc gaggttctaa gttggagatg gttagaaacc
     2401 ggtgatgacc ttaccctgct tcggtcattg ccggggccac gtgcctgacc agcaaccgga
     2461 tcagcagtcc gggcccggtt tagcccctct agtttgggcg aacctatagg ttaccatagt
     2521 tctccccatt gggcaatcgt ggcgatctcc ttacgggacg gccctcagcc gctcgaatac
     2581 agatttcggt agatttcata cacttcaaag atgacccgaa tgtagagttg gggctgatcg
     2641 aggcggatca cttccaggaa gaggtctgga tccgaggggg ccagctcttt ccagaactgg
     2701 gcgttgcccg gaccggcgtt gtaggccgcc aaggcggcga acagatctcc ctcgaagcga
     2761 tcgcgctggg tcgccaggta gaaggtcccg aatcggacgc taacgatcgg ccggtacagg
     2821 tcagcactgg tgtagtctgg gggccaaccg agctgggcgg cgatcgactc gccggtgctc
     2881 gggatcacct gcatcaggcc gtgggcggcg gccgatgacg tggcgaaccc ctcgaagaga
     2941 ctctcctgtc ggacgaccga aagcaggaac agcccgtcca ggccatagcg ggcggcctcc
     3001 ggcaagatca gctctccgaa gtaggggcca aaccggatcc gattgaagta cacaggtgcg
     3061 tccagcgtcg ccgcattgtc catcccagcc agcctcagga tctgccggga ggaaaaaatc
     3121 gcctcccggt acagccccaa gccgagcagc tcatgcatga ggcggtaggt cgcctccggg
     3181 tcgctctcga ctgattgccg caacgacgag aactcggccg ccgcgagctc cagctccccg
     3241 agttcccata gttcacggcc gcggagatag cgttgatctc cggtaatggc caggtttggc
     3301 tcactcaacg gatccggccc aagcaggctg aaggtctggc gtagccactg ctcggcctcg
     3361 gagcgttcag tttccaggtc gtaggcaaag tcgaacgccc cactgccttc gaacaccgga
     3421 ctaccatcca gcagctccgc cgccctgacc gagtagtatc cggttggatc tgcctccacc
     3481 gctcggttcc aggcggcctc cgccgcttgc aggttgccgc cggcttgctg ggtttttccg
     3541 atccagagct ccgccgccgc agcatctgcc gaggttattg cattggccaa ggcgctcttg
     3601 aaggcgagat ccgcctcggc gaatcgccca agccgaaacc atgccacccc agcgaggaag
     3661 gcagcttggt gagtcaggtc ttggcccgga tacagctccg gcaaccggag ccagatctcg
     3721 gctgcccgtt gcaggtcagc cccccgctct gccgtgcggc ccgcggcgaa cagcgcttcg
     3781 gaaccgattt caggctctgg ccgagtggcg acgagcgcca agtaggtgtc gacagccgct
     3841 gaatactgat caagataggc ccactgggtg cgggccttgg ccagtgccga atcgggccac
     3901 agggcgtcgt ccgggaagtt ggtgaccacc cattcgaaat cggctagcgc ctgctgggag
     3961 ttcccgagtg ccaagtggct caagcctcga taatagaagc ctgccgcgct gggcacactt
     4021 cccagcacgc gatcgaatgc cgccaccgcc ggctcgtagg ccccggcctg ataatccacc
     4081 agaccgcgtt ggaactcgtc gaccgggatc ccggcctcca ccaaccgaat gagggcaatg
     4141 tagctgtcat aggcggtggg gtagttgctt accgagtcca tgtagcggga gacagctccc
     4201 tcgaagtcgc ccagcccctc cagggtttcc ccggcaagca aattcatcga agctcgggtt
     4261 cctgggtcgg tagtctgagc ggagatcgag tcaaactgag tcagggcgag ctcgagctcg
     4321 ctagcttcga gatagcaccg gccaatcttc accagcagcg ggaggacgtc tgtgatgcgg
     4381 ggtgcggcga tcgcattctg gtaatgaccc actgcttcca acggttttcc cgccagccgc
     4441 agcgcgtcgc cgagccgttc ctgcacatag gcatccagca ctcccggccg catctccaga
     4501 taggcggcgt agtccatcgc agaaagctcg agctggccaa gaccgagcaa cgcttgggct
     4561 cgcaggagcc gggcctgggc cgaccgctcg cttgttggaa atcgttcgat caattcgctg
     4621 aattcaactt gggcgccggc gtacagtccg gattgcaaca aggcttcccc tcgcccatag
     4681 agggcctccg cgatctcttc cggtccgccg gcttgctcaa gccgccggct gaattcaacg
     4741 tacgcctggt cccaatctcc a
//
LOCUS       DATDPQ010000267         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_649222, whole genome shotgun sequence.
ACCESSION   DATDPQ010000267 DATDPQ010000000
VERSION     DATDPQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649222"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VI520_06055"
     CDS             complement(<1..1261)
                     /locus_tag="VI520_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF37478.1"
                     /translation="MAKKKIESSKQPSRAEPVEEPIRQPVPPSAQGPALPTARPPDVI
                     EPELTAPQRPELAPAAAPDETPSGVRAKTDFGSVTVALEGARPSAQVMADSSEAFRES
                     LQTLRGALELLIYGKVPDLRQSKKFLNIAFREAEYLGNRVHDLQIASLLEAGRLRLKL
                     TALNLPELLGTIVEKLSPGAAEFGAALKLETPQELPLIRGDEGLLRVLLTNLIERSLK
                     AAPANATVHIRLEPIGDRLRLQVLSYVGATSDLAPITLDESHERGLALYVAERIAYVH
                     EAELVPQGIDGELRGFHLTLPVQLKGRGRGRILVVDDNPQAATLLEYALQEEGFEAIK
                     ALNGLEGLKLARSERVDLVILDILLPGIDGFEVCHRLRSSPETASTPVIMISAKSREE
                     DRATALRIGADAYFGKPLGMAELMVAIE"
     gene            complement(1261..1650)
                     /locus_tag="VI520_06060"
     CDS             complement(1261..1650)
                     /locus_tag="VI520_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF37479.1"
                     /translation="MGPNSQPSRSTPRLSSIHPKSSKRCQTGASTRRRNQIGANDVSR
                     RVSIVAEVQVTNGEPNASRGRILNARDAVEYLGISLSTLNRIEKQGLLVPFRTPGGHR
                     RYAQKMLDEYLESSRRLPDDDGVGEIE"
BASE COUNT          321 a          615 c          700 g          383 t
ORIGIN      
        1 tctcgatggc gaccatcaat tcggccattc ccagcggctt cccgaagtag gcgtcggcgc
       61 cgatccgcag cgcggtcgct cgatcttctt cgcgggactt ggccgagatc atgatcaccg
      121 gggtggaggc ggtctccggc gaggagcgca gccggtggca gacttcgaag ccgtcaatcc
      181 cggggagcag aatatcgagg atcaccagat cgacccgctc ggagcgggcg agcttgaggc
      241 cctccaggcc gttcagggcc ttgatggcct cgaagccctc ttcctggagg gcgtattcga
      301 gcagcgtggc cgcttgcggg ttgtcgtcca ccactagaat cctgccccgg ccgcggccct
      361 tgagctggac cggcaaggtg agatggaacc ccctcaactc gccgtcgatc ccttggggaa
      421 cgagctcggc ttcgtggacg taggcgatcc gttcggcgac atatagtgcc aggccgcgtt
      481 cgtggctctc gtccagggtg atgggcgcaa gatccgaggt ggcgccgacg tagctcagca
      541 cttgcagtct gagccggtcg ccgatcggtt cgagccgaat gtggaccgtc gcgttggctg
      601 gggcggcctt caggctgcgt tcgatcagat tggtgagtag gacccgcagc aggccctcat
      661 ccccccggat cagtggaagt tcctgggggg tctcgagctt cagggcggcg ccgaactccg
      721 ccgcgccggg agagagcttt tcgacaatcg tgccaagcaa ttctggcaga ttgagggcgg
      781 tgagcttgag ccggagtcgg ccggcctcca gcagcgaggc gatctgcaga tcgtggaccc
      841 ggttgccgag gtattcggct tcccggaagg cgatgttgag aaacttcttc gactggcgca
      901 ggtcggggac ctttccatag atcagcagtt ccagtgcgcc gcgcagggtc tgcagcgact
      961 cgcggaacgc ctccgaggag tcagccatga cctgggcgct ggggcgggcg ccctcgaggg
     1021 cgaccgtgac cgaaccgaag tcggttttgg cccggacgcc ggacggagtt tcgtcgggcg
     1081 cggcggccgg ggccagctcc ggtcgctggg gcgcagtgag ttcaggctcg ataacatccg
     1141 gcgggcgggc ggtcgggaga gctggccctt gggcactcgg cggcaccggt tgccggatcg
     1201 gctcctcgac gggttcggct cggcttggct gcttgctgct ttcgatcttc ttctttgcca
     1261 tcactcgatt tcccccaccc catcgtcatc cggcaatcgg cggctggatt ccagatactc
     1321 gtcgagcatc ttctgcgcgt agcgccggtg tccgcctggc gtccggaagg gaaccagcaa
     1381 gccctgcttc tcgatccggt tgagtgtgct gaggctgatc cccaggtact cgaccgcgtc
     1441 gcgggcgttg agaatccgtc cgcggctggc gttgggctca ccgttggtta cctgaacttc
     1501 agcgactatc gagaccctcc ggctgacatc gttcgcccca atctgattgc gacgcctagt
     1561 gcttgcgccg gtctgacatc gtttggatga cttgggatgt atcgagctta agcgaggggt
     1621 ggagcgagaa ggctgggagt tgggtcccag ttaagtgatt gtaaggtgga ggagtggcgg
     1681 gcaggacggt gccctctcga tccctccaga tcgagccttg ccccggccgg ccccgggccg
     1741 gcgagcccga gttgtcgttt cctcattgac tccaccccga cccgcatgat agagtcaagg
     1801 cactcatggt actggggtac catgcgcctg gaggggtccc gggctcaatc gaagcggcct
     1861 agttgacgag ccagccgccc aatagatacg ccggctggca ggcggctagg gtcgaacagc
     1921 agtgtggagg cagcgagccg agggcctaga ttggtcgctg gccggtcaga ccggccgcac
     1981 ctcggggagc cactagcgaa accggccaca gcgtggtcg
//
LOCUS       DATDPQ010000268         3386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3291607, whole genome shotgun sequence.
ACCESSION   DATDPQ010000268 DATDPQ010000000
VERSION     DATDPQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291607"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..1334
                     /locus_tag="VI520_06065"
     CDS             594..1334
                     /locus_tag="VI520_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37480.1"
                     /translation="MDLNLVLPFISTVIMLVFAVSVFGRWLARGKPHFLYWSIGLGMF
                     GAGSFAEAYFAGVGWSRLVFFIWYFFGAVLNAAWIGHGTLLLLFRRRWTTVLTGLLIV
                     GSLAALALMLQVMPRLDAGAFNPDLPISEQYREIMPTVSEGATIRLTTPFFNIYGLIT
                     LVGGALWSAWLFLRKQVLPNRVLGNVLIAAGALSIGLASTLTRLGYGGYLYLGELVAA
                     VLMYSGFVLAARPATGEEPVPEPASSPA"
     gene            1356..2693
                     /locus_tag="VI520_06070"
     CDS             1356..2693
                     /locus_tag="VI520_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HLF37481.1"
                     /translation="MTNGLAEPRFRLLRTIKLGSFHIGSAAADLMVTAVWNRIMIVDL
                     GAPAWPVALLSAARYFLAPLSLWAGHRSDHRPLLGRRRISYIWLGRILMLISLPLLPV
                     SAKLLAEQPGSAGGWSLTVVSFVLFGSGTLISGPPFLALVHDSVPYARRGQAVSIVQF
                     MLVASFAVLPLTFARLLPSYEPEAFSSLVSLVVIGAGLAWFGSVWREDRAPAKRTSSA
                     EPDLRQVFGGIWSDRRSRRYALFLGVSAVFAFMQDAVLEPFGGDVFRLSVGETTRFNA
                     YWGSGVLLAMLLTMWLTRRWRPERQVHATRWGLLLLALPLLGLGAASAVESFSPIRPL
                     LFLFGLGFGVFTVGGVSLLMAMSKEGRAGAYLGLWSVIQLVGRGAGIAAGGLIRDVAL
                     SLTGGFAGAYASVFWIEGLGALVCIWLLGRVDVLGFAGERGQVGRVEALAAAD"
     gene            complement(2719..2791)
                     /locus_tag="VI520_06075"
     tRNA            complement(2719..2791)
                     /locus_tag="VI520_06075"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2756..2758),aa:Asn,seq:gtt)
     gene            2850..3020
                     /locus_tag="VI520_06080"
     CDS             2850..3020
                     /locus_tag="VI520_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37482.1"
                     /translation="MSEDLRSSVEPGGSRRRIGLVVLAVAGLLLCCCGSFVLAWKFGD
                     PVLEMLQAFFQP"
     gene            complement(3021..>3386)
                     /locus_tag="VI520_06085"
     CDS             complement(3021..>3386)
                     /locus_tag="VI520_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HLF37483.1"
                     /translation="PDKDFHGLSLRKGDRFIEQHFADRWYNVFAVHDGTSGRLKGWYC
                     NVSRPAQIADDQVSAEDLALDLIVRLDGSWTVLDEPEFEAQGLSAGDRANALAALAEL
                     KHLAQASEGPFRLDWARKR"
BASE COUNT          505 a         1004 c         1120 g          757 t
ORIGIN      
        1 actggttggc cgttgggctg cattaccggc cagggcattc aggcggcccg ctggttgact
       61 cggccggccg gctggtcggg atcaacacgg tcatgctggg tcccgatgtt gggttggcgg
      121 tcccggtgca tgaagtcaaa gctttcctga agcgcgcgat cgcaaggatc tgattgcgct
      181 cggaaagcca gaacacaggc ctccgctcgg cggaggcctg tccatttaac ttccacactc
      241 atactggtct aaggacgggc cgtgcagaat cgcgttataa tctccgcgat tctaccgaag
      301 tcctcacaaa ctagtagatt tctatagatt ccgacttggg cttcagtggg caacacaccc
      361 cgcaaacggg gcgggcgaag tcggcccccc cgggttggct ggtttcctcg gcgaaagaaa
      421 ggcacctccc gggttccctc cgggtcggct ttttactcgc gggtgctgcc cgccctgatc
      481 ctcgtgttag gcggtttgac tgccgttctc atactcttcg cgttcggcat cttgattggg
      541 gtcatcccgt tccggtaagg aactgctagg ctcaggcctg aggagaaaac gcaatggatc
      601 tcaatttggt gctcccgttc atttctaccg tgatcatgct cgtgttcgcg gtgtccgtgt
      661 tcggacgttg gttggcgcga ggcaagccgc actttctcta ctggtccatc ggcctcggga
      721 tgttcggggc cggcagtttt gccgaggcat acttcgcggg ggtcggttgg agccggttgg
      781 tgttcttcat ctggtacttc ttcggcgcgg tgctgaatgc ggcgtggatt ggccacggca
      841 ctttgttgct gctcttccgc cggcgctgga cgaccgtctt aaccgggctg ttgatcgttg
      901 gcagcctggc cgccctggcg ctcatgctgc aggtgatgcc gcggctggac gccggcgcgt
      961 tcaacccgga cctgccgatc agtgagcagt accgcgagat catgccgacc gtaagcgagg
     1021 gggccacgat ccgtctgacg actccgttct tcaacatcta tgggttgatc acgctggtcg
     1081 gcggtgcact ctggtcggcg tggctgttcc tgcgcaagca ggttttgccg aatcgggtgc
     1141 tcgggaacgt cttgattgcg gccggggcgc tctccatcgg gttggccagc acgctgaccc
     1201 ggctgggcta tggtggttac ctgtacctgg gtgaactggt ggccgcagtc ttgatgtact
     1261 ccggattcgt gctcgccgct cggccagcca ccggcgagga gcccgtcccc gagcccgcct
     1321 cgtcgccagc ctaattgacc tctggagccc cgggcatgac taacggactg gccgagccgc
     1381 gcttccgcct gctgcgtacc atcaagctcg gcagttttca catcggctcg gccgcggccg
     1441 atctgatggt gacggccgtc tggaaccgga tcatgatcgt cgatctgggg gctccggcct
     1501 ggccggtcgc gctgctcagc gcggcccgtt atttcctcgc gccgctctcc ttgtgggcgg
     1561 ggcatcgctc cgaccatcgc ccgttgctgg ggcgtcggcg aatcagctac atctggctgg
     1621 gccggatcct catgctgatt tcgctccccc tcctgcccgt ctcagccaag ctgcttgccg
     1681 agcagcctgg atcggccggg ggctggagtc tgaccgtggt ttctttcgtt ctcttcggca
     1741 gcgggacctt gatttcgggg ccaccttttc tggcattggt gcatgacagt gtgccctacg
     1801 cccggcgggg gcaggccgtc agcatcgtcc aattcatgct ggtcgcgagc ttcgctgtat
     1861 tgccgctcac ttttgccagg ctgctgccga gctacgagcc cgaggcattc tcgagcctgg
     1921 tctcgctggt ggtaatcggc gccgggttgg cgtggtttgg gtcggtctgg cgggaggacc
     1981 gagcaccggc aaaacggacc tcgagcgccg agccggactt gcggcaggta ttcgggggaa
     2041 tctggtcaga ccgacgttcc cgccggtacg ccctgttcct gggggtttcg gcagtattcg
     2101 ccttcatgca ggacgcagtg cttgagccct tcggagggga tgtcttccgg ttgtcggtcg
     2161 gagagaccac tcgcttcaat gcctattggg gctctggagt gctgctggcg atgctgctga
     2221 cgatgtggct gacccggcgt tggcgtccgg agcgtcaggt gcatgccacc cgttggggat
     2281 tgctcctgtt agcgctgccg ctgctgggtt tgggggccgc gtccgccgtg gagagttttt
     2341 ccccaattcg gccgctgttg ttcctgtttg gcctcggctt tggagtcttc accgtcggcg
     2401 gggtaagcct gctgatggcg atgagcaaag aaggccgggc tggagcctac ctcggcctgt
     2461 ggtcggtgat ccagttggtg ggaagagggg ccgggatcgc ggcaggcggc ttgatacggg
     2521 atgtggccct gagcctgacc ggaggcttcg cgggggccta cgccagcgtg ttctggatcg
     2581 aggggcttgg tgcgctcgtc tgcatctggc tgcttgggcg ggttgacgtg ttgggctttg
     2641 ccggggagcg aggccaggtt ggccgggtcg aagccctcgc ggcggccgac tagttcaggg
     2701 cggaaacgtc caaagaagct cctcggccag gactcgaacc tggaacctag cggttaacag
     2761 ccgcccgctc tgcctattga gctaccgagg aaggcccagc gattatactc aaagggccaa
     2821 ctgaccaatt cctgatataa atccaatcta tgtcagagga tctgcgatcg agtgtggagc
     2881 cgggcggatc caggcgaagg atcgggctgg tcgtcctcgc agtagccggg ttgctgctgt
     2941 gctgctgcgg ctcgttcgtc ctagcctgga agttcggcga tccggtgttg gagatgttgc
     3001 aggctttctt ccagccctaa ctagcgcttc cgggcccagt ccaatcggaa aggcccttcc
     3061 gacgcctggg caagatgttt gagctcggcc agggcggcga gggcgttggc tcggtcgccg
     3121 gcagacagtc cctgagcctc gaattccggc tcgtcaagca cagtccaact cccatccagc
     3181 ctcacaatca agtcgagcgc cagatcttcg gccgagacct gatcgtcagc gatttgggcc
     3241 gggcgagaga cattgcagta ccagcccttg agccggccgc tggttccatc atgaacagca
     3301 aacacgttgt accaacggtc ggcgaagtgc tgctcgataa atcggtcgcc cttgcgcagg
     3361 gacagaccat gaaaatcctt gtcggg
//
LOCUS       DATDPQ010000269         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_133219, whole genome shotgun sequence.
ACCESSION   DATDPQ010000269 DATDPQ010000000
VERSION     DATDPQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133219"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VI520_06090"
     CDS             complement(<1..971)
                     /locus_tag="VI520_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLF37484.1"
                     /translation="MRRRLSTLAWFGAALAALTAAPAPDLAEDLLSQMTPEQRVGQLF
                     LVTFRGASPNQDDPIFGLIRDQYIGGVVLRRAEDNFVGPPDTLPAARALTSALQTATY
                     EAAAGSEGAVAYIPLLIGLSQEGNGPPYSEIMSDLSELPSQMAIGATWDPGFARQVGE
                     RLGTELASLGFNLLLGPSLDVLVDPTQLALADLGVRSFGGDSYWVSVMGRAFIEGVHL
                     GSQGRLGVVPQHFPGLGGTDRPIREEISTVRKSLVQLQQIELEPFFAVTAAGPGEDPA
                     IADGLLTGHIRYQGFQGNIRDTTRPIGLDPDAFGQLMALDPFAAWRQA"
     gene            complement(1050..1913)
                     /locus_tag="VI520_06095"
     CDS             complement(1050..1913)
                     /locus_tag="VI520_06095"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HLF37485.1"
                     /translation="MEGLQTKPPLVVHSPHPMAYSVDELICVCISRRIKNGDVVALGL
                     ATPIAAVGALLAQQTHAPAIYLASAIGQCFCRSGPRLELQGAEGSWLAHSMGSFGFAQ
                     AAADFLPAAKPLEFFRPGQVDRHGNFNNLAIGRDIRRPRLRMPGSGGIPDVTVLLDQI
                     HLYVPRHSKVTFVPQLDFLSGLGHTPGRRLGAGPVYLVSDLGQFDFAEGELRLTHVHP
                     GVRAEDVQAKTGFELRVAEQLAVSEPPSAHELGLLREEIDPLGLRRLELLGGGRRREA
                     LREILEAEAAP"
     gene            complement(1969..>2472)
                     /locus_tag="VI520_06100"
     CDS             complement(1969..>2472)
                     /locus_tag="VI520_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37486.1"
                     /translation="PFQVKANGGVFLIDDFGRQQVRPRDLLNRWIVPLENRIDFLTLH
                     TGRKVEVPFDVLVVFSTNLPPKDLVDEAFLRRLRHKIEIGDPSYEAYRDIFKRTAEAK
                     GVPYSDRGLAYMLQEWYIKRNRKLRASHPRDICDQILDISRYLNVEAAMSKELIDKAA
                     EAYFVEL"
BASE COUNT          491 a          791 c          757 g          433 t
ORIGIN      
        1 gcctgccgcc aggccgcgaa gggatcgagt gccatcagct ggccgaaggc atcgggatcg
       61 agtccaatcg ggcgggtggt atcccggata ttgccctgga atccctggta acgaatatga
      121 ccagtgagca gtccgtcggc aatcgcgggg tcctcgccgg gcccagctgc cgtgaccgcg
      181 aagaatggtt caagctcaat ttgctgaagc tgcaccaggg acttccggac ggttgagatc
      241 tcctcccgga ttgggcggtc ggtgcctccg aggcctggaa agtgctgggg gacgacaccc
      301 agccgaccct gcgatccgag gtgcactcct tctatgaacg cccggcccat cacgctgacc
      361 cagtaggagt cacccccgaa gctcctgact cccagatccg cgagggcgag ctgggttggg
      421 tctaccaaga catccaatga ggggcctagc agcagattga aacccagcga cgcgagctcg
      481 gtccccaatc tctcccccac ctgacgggca aacccaggat cccaagtcgc cccgatggcc
      541 atctggctcg ggagctccga gaggtcgctc atgatctccg agtacggcgg cccattccct
      601 tcttgactga ggccgattag cagcgggata tatgcgaccg cgccctccga acctgccgcg
      661 gcttcgtacg tggcggtctg gagggccgag gtcagtgccc gggccgcggg cagggtatcg
      721 ggcggcccga cgaaattatc ctctgccctc cgcagcacca ccccgccaat gtactggtcg
      781 cggatcaagc caaagattgg atcgtcttgg ttgggtgaag cccctctaaa agtgaccaaa
      841 aacaactgac cgacccgctg ctccggggtc atttgcgaaa gcagatcctc agccagatcg
      901 ggcgcaggcg cggctgtcag cgccgcgagc gccgccccga accaggcaag ggtggaaagc
      961 cttcgcctca tctcaggaag tatacgcatt ccgccgactt tgggctgagc agccgatcga
     1021 aagggaagtg cgggtagctt ggaggagatc tagggggctg cttccgcttc aaggatctcc
     1081 cgcaaggcct ctcgccgcct gccgcctccg agcagctcca ggcgacgaag acccagggga
     1141 tcgatctctt cgcgtaacag acccagctcg tgcgcgctcg gaggctcact gaccgcgagc
     1201 tgctccgcga cccgcagctc gaatccagtc ttagcctgaa cgtcctccgc ccgcaccccg
     1261 gggtgcacgt gagtcaatcg cagttcgccc tccgcaaaat caaactgacc cagatcagac
     1321 accaggtaga ccgggcctgc tccgagtcgc ctgcccggcg tatggcccag ccccgacaga
     1381 aagtccaact gtgggacgaa agtgactttg gagtgccgcg gcacatacag gtggatctga
     1441 tcgaggagca cggtcacgtc gggaattcca ccactgccgg gcatgcgcag ccggggacgc
     1501 cggatgtccc gtccaatcgc caaattgttg aagttaccat gccggtcgac ctggccgggc
     1561 cggaagaact ccaacggttt cgccgccggc aagaagtcgg cagccgcctg ggcaaatccg
     1621 aagctcccca tcgagtgtgc cagccagctt ccctccgccc cctgcagctc cagccgaggg
     1681 cctgaccggc agaagcactg cccaatcgcg gaggccaggt agatcgccgg tgcgtgagtc
     1741 tgttgggcaa gcaaggcgcc caccgctgct atcggggtgg ccagccccag agcaaccaca
     1801 tcaccgttct tgattcgccg cgagatgcaa acgcagatca gctcatcgac cgagtaggcc
     1861 atcgggtgcg gtgagtggac gaccagtggg ggtttggttt gaaggccttc catgttcagc
     1921 caacttgccc tgcgcgctgc ggggcagcgg agacgggtgc cggaggagct acagctctac
     1981 gaaataggcc tcggcggcct tgtcgatcag ctccttgctc atcgccgcct cgacgttcaa
     2041 gtagcgcgag atgtcgagga tttgatcgca gatgtctctg ggatgcgaag cccgcagttt
     2101 gcgattgcgc ttgatgtacc actcctgaag catgtaggcc aggcctcggt ccgagtacgg
     2161 aacccctttg gcctctgcgg tccgtttgaa gatgtcccgg taagcctcgt aggacgggtc
     2221 tccgatttca atcttgtgac gcaatcggcg aaggaacgct tcatctacca ggtccttggg
     2281 aggcaggttg gtcgaaaaca cgaccaacac gtcgaatggg acctccacct tgcgcccggt
     2341 atgcagagtc aggaaatcga tccggttctc gagcggaacg atccagcgat tgagcaagtc
     2401 gcgcggccgc acctgttggc gtccaaagtc gtcaatcagg aagacccccc cattcgcctt
     2461 cacttgaaag gg
//
LOCUS       DATDPQ010000270         3091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1966571, whole genome shotgun sequence.
ACCESSION   DATDPQ010000270 DATDPQ010000000
VERSION     DATDPQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966571"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VI520_06105"
     CDS             complement(<1..939)
                     /locus_tag="VI520_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLF37487.1"
                     /translation="MAFNPLNWLLGLFSLDIGIDLGTANTLVHVRGKGIVLNEPSWVA
                     IEKRSKRPLFIGAEAKEMVGRTPANVVALRPLRDGVISEFDITEAMLEYFIGKAHEQS
                     IAPVPRPRVVVGIPSGATEVERRAVFDATMAAGARETYLIEEPTAAALGAGLPIGEIG
                     GSMIVDIGGGTTEVAVYSLGGIVTSRSLRVAGDEMDQDIIGYVRNKHNLLIGERMAER
                     VKITMGSASPIKDEQTMTVRGRNLVSGLPEAVEVSSIEIREALAGSVKIILDTLKDTI
                     DEIPPEIIADLMEVGICMAGGGSQLKGLDTRLSEELN"
     gene            1058..2470
                     /gene="tilS"
                     /locus_tag="VI520_06110"
     CDS             1058..2470
                     /gene="tilS"
                     /locus_tag="VI520_06110"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLF37488.1"
                     /translation="MDLTQQIGDYIAREALLEPKQRLVVGVSGGADSLCLMDSLHRLG
                     YRLVVAHFDHRQRPESTSEAETVRMLGASYGLPVEIGSMDPPRDRGSTEQLARIERYR
                     FLARVATRRRIRTLAVGHTADDQVETVLMHLLRGAGPPGLRGMLPRTRLDAWMGNLRA
                     PALTLIRPLLELRRAQTVAHCATHGLVPLDDPSNRDLRFFRNRLRHELIPRLLEYSPG
                     IEATLLRTAKLMAAETELVDDLVELNWSSWVRELGPGALSLNRERLLAAPLSLRRAAL
                     RRAIQTLRPEQRDIGFEAIERGLASLRDSRRQTVIGQLELLPLRGDLILKTITAPLAF
                     PDLPQLRTPAVRRLRIPGTVRLANRWEIEFRSVRRSSRRGIRAGQVRFDLDRLPDKLV
                     LRAPRPSDRLQPVGMHGTLKLADLFINRKVPKLARARWPVLVSGEDLIWIPELHRSRI
                     ARPSSKSKRLLELTLRRPAE"
     gene            2573..>3091
                     /locus_tag="VI520_06115"
     CDS             2573..>3091
                     /locus_tag="VI520_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HLF37489.1"
                     /translation="MAHYLGGLYRLGGHESPISLSAVAEEVEVTAPAAARMFGRLEAA
                     GLVEREPYRGVRLTPRGALEALREIRSHRLSEAFLVRVMEYGWHEAHDLADHLAEIGD
                     DDFIDRMEAKAGFPRRCPHGEPIPSRDGKLPVLKDLPLTELAQGEHGTISRVRIRDSE
                     KLEYLAKEGLLPE"
BASE COUNT          561 a          983 c          965 g          582 t
ORIGIN      
        1 attgagttcc tcggaaagtc gggtgtccag tcccttcagc tgagacccgc cccccgccat
       61 gcagatccca acctccatga ggtcggcgat gatctcgggg gggatctcgt caatcgtgtc
      121 tttcaaggta tcgagaatga ttttgaccga gccggcaagc gcctcccgga tctcaatcga
      181 agacacttca accgcttctg gcagcccgct gaccagattt cgcccccgaa cggtcatggt
      241 ctgctcgtcc tttatcggag aggccgaacc catggtgatc ttgacccgct cggccatccg
      301 ttcgccaatc aggaggttgt gtttgttccg gacgtaacca atgatgtcct ggtccatttc
      361 atccccggcg acccgcagcg agcgggacgt cacgataccg cccagcgagt agaccgctac
      421 ctcggtcgtc ccgccgccga tatccacgat catcgatccg ccgatttcgc cgatcggcag
      481 tccggctcca agcgccgcgg cggtcggctc ttcgatcagg tacgtctctc gggccccggc
      541 ggccattgtg gcatcgaaga cggcccgccg ctcgacctcg gtggctccac tagggattcc
      601 aaccacaacc cgcggtcggg gaaccggggc gatgctctgc tcgtgggcct tgccgatgaa
      661 atactccagc atcgcttcgg tgatgtcgaa ctcggagatc acgccgtccc ggagcggccg
      721 caaggcaacc acattcgccg gggtcctgcc gaccatctcc ttcgcttccg ctcctatgaa
      781 gagtggtcgc ttggaccgct tttcgatggc cacccaagag ggctcgttga gaacgatccc
      841 ctttccgcga acgtggacga gggtgttcgc agttccgaga tctattccga tatcaaggga
      901 gaatagtcca agcagccagt taaggggatt gaaagccacg ggtaagcccc tccggctgaa
      961 gggcgagcgg gattatacca tgcggtgccc gaaggccaat tcttactgct tatgttagga
     1021 aaactctcgt cgccccggca gtataatcgg ctgttccatg gatctgaccc aacaaatcgg
     1081 cgactatatc gcccgcgagg cgctgctcga acccaagcag cgccttgttg tcggagtgtc
     1141 cggcggggcg gacagcctct gcctgatgga cagcctgcac cgtctcggtt accgcctggt
     1201 ggtcgcccat tttgaccatc ggcagcgccc ggagagtact tctgaagcgg agacggttcg
     1261 catgcttgga gcttcctacg gcctgccggt ggagattggc tcaatggatc ccccccggga
     1321 cagagggtcc acagagcagc tcgctcggat cgagcgttat cgcttcttgg cccgggtcgc
     1381 cacccggcgc cgaatccgaa ccctggcagt cggacacacc gcggacgatc aagtcgagac
     1441 cgtgctcatg cacttgctgc gcggcgccgg gccacccggc ctgcggggga tgctgccgcg
     1501 cactcggctg gacgcctgga tggggaacct cagagcacct gctttgactc tcatccggcc
     1561 cctgctggag ctgcggcggg cccagaccgt cgcccactgc gcgacacacg gactcgtgcc
     1621 tctggacgat ccgagcaacc gggatctccg attctttcgc aatcgacttc gccacgagct
     1681 gattccccgg ttgttggaat acagtccggg catcgaagcc acgctgctgc ggaccgccaa
     1741 gctgatggca gccgagacgg agttggtgga cgatctcgtc gaattgaact ggagcagctg
     1801 ggttcgggag ctcggtccgg gcgcactgtc gctgaaccga gagcggctgc ttgccgcgcc
     1861 gttgagcctg cggcgggccg cgctgcgaag ggcaatccag accctacggc cggagcagcg
     1921 ggacattgga ttcgaggcga tcgagcgggg actggccagc ctgcgcgaca gccgccgcca
     1981 gacggtgatc ggacagcttg aactgctgcc gctgcggggt gacctgatcc tgaagaccat
     2041 taccgcaccc ctggcgtttc ccgacctgcc tcaacttcgg acacccgccg ttcgacgact
     2101 gcgtatccca ggtaccgtaa ggctggcaaa ccgctgggag atcgagttcc ggtcggttcg
     2161 aaggagcagc cgacggggca taagagcggg ccaggttcga ttcgacctcg accgtctgcc
     2221 tgacaagctt gtccttcggg caccccgacc ctccgaccgg ctgcagccgg tcggaatgca
     2281 cggcacgctc aagcttgcgg atctgttcat caatcgtaag gttcccaagt tggcccgggc
     2341 ccgatggcca gtgctggtca gcggcgagga tctgatttgg atcccggaac tgcatcggtc
     2401 tcggatcgca cggccctcga gcaagtccaa gcgcctgctg gaattgactt tgaggcggcc
     2461 cgcagagtaa gccaaaccgg ctgcctatgg taaactgctc ccactcagtg gttcacacga
     2521 cgaagcctca ttgagctcat cgaaaactga gaccgagcgc gtatctgcga gcatggccca
     2581 ctatctgggg ggcctgtatc ggctcggcgg acacgaatcc cctatctccc tctcggcggt
     2641 tgctgaggaa gtcgaagtta ccgccccggc agcggctcgc atgtttggcc ggttggaagc
     2701 agccgggctg gttgagcgag aaccctaccg cggggttcgc ctcactcccc ggggggcgct
     2761 cgaggcgctg cgggagatcc gatcgcatag gctgagcgag gccttcctgg tccgagtgat
     2821 ggaatacggt tggcatgagg cccatgacct ggccgatcat ttggccgaga tcggcgatga
     2881 cgacttcata gaccggatgg aggcgaaggc cggttttccg cgtcgttgcc cgcatggtga
     2941 gccgattccg agccgggatg gcaagctgcc ggtcctcaag gatttgcccc tgaccgagct
     3001 agcccagggc gagcacggga cgatcagccg ggtgcggatt cgggactcgg aaaagctgga
     3061 gtatctcgcc aaggaaggcc tgctcccgga a
//
LOCUS       DATDPQ010000271         4451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_133670, whole genome shotgun sequence.
ACCESSION   DATDPQ010000271 DATDPQ010000000
VERSION     DATDPQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133670"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VI520_06120"
     CDS             <1..397
                     /locus_tag="VI520_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37490.1"
                     /translation="RRGSRWRRAEMEIEFLDPAEAPLPPDQMYFRSVEIEPHSDGQRL
                     KVSLQVAPFQVRPSIDLTVTDDQGKVVASSSIVEATETSMSVILHLRDRSSKPGHRLT
                     ASLVYPDREPVDERILEFALPDPDPSEAS"
     gene            406..3567
                     /locus_tag="VI520_06125"
     CDS             406..3567
                     /locus_tag="VI520_06125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HLF37491.1"
                     /translation="MASRDPLIRHAIAQPAFDERISLHRERLVDAIHANIPRKLIAIA
                     APAGYGKTTLLADFGSSTDLTVCWARLTQADWDIMRFAQVLAASLQSHFRRLKGQPDL
                     AALATASPEALALAFSVAISENLTEAFVIALDDIHLINQSKPVLAFLDRFLQELPEQV
                     TVIAAGREVPEVSLARLMAERDLAGFGPQDLALTREELIDLTQRQRGAPLSDFEIDRL
                     LEESRGWVTGIVMSGTLADRGLGSLVSSGRPMMFEYLASVVLNQQPDELRRFALDAAV
                     LPIMTDESCNVVLGREDSRRLLTQLVRKALFVTATEGPLRTYEFHPLFREFLLASLQG
                     ADVKRIVDLRKRAANYHLSRGSLEQAVDLLIEAGSVRQAAKTAERHARELFESGRVQT
                     IERWVERFRNEGMIVPSLTLELANSVRNRGNLSGAEALLGEAERAISPRSARDLQARF
                     EIARGYLWWQLGKHDKALEAADRVDHLFAKRANRHYQGIGKNIRALALAHGRGEFATA
                     EQLLDEAAQLLAHSENEFGVWTVLIDLIMVQDAQGKAQLANRTRERALSLARKIGAPL
                     PLAVALGNRAMGAHQEGEYELALELGREALKQARQAASPLREAISLLRQADVFNDIGL
                     ALQAAELYGEALSILTRLDNVEWIRYACTQTSVLHRRRGGASLAHEWLRRAMLADESK
                     VAPSEIRVQFAALEIQAAPEQAIKSIRSLLKRRATSLEAGEVTLVQYFQAVAELKLTH
                     PEQAVELLSQAFTQAGRSGTEQVIAAELRFDAAMREFAVGHFGTDPTYSVIRSRIEMM
                     EALSDRHELTTEAEPEGVRIEVQALGSSRILVGGIEPRDLKPLTREILFYLIDRERVR
                     RDKLMEEFWPDQSSGRQVTSLHTAIYGIRSCMGKDSVLFDGTVYQIDPSTPVEYDVAR
                     FEKAALVAERLLPGDPRLLFALTEAVRSYQGDFHPELDRAWVDERRRTLELKYLDLLA
                     SYSQEALVRDRPSEALDLLRQAIRLNPLRDDTNRYYMEALGRLGRRSELVAHYQDYVQ
                     LLRQELALDPPPHLTELYDRLIS"
     gene            3979..4263
                     /locus_tag="VI520_06130"
     CDS             3979..4263
                     /locus_tag="VI520_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37492.1"
                     /translation="MQSLNQLVARSIIDPGVVQAFRAGRIGDVLTEVGFTPDLQAQLT
                     GIECDSWAEFAVISYRHVRAAEAQAPRLQFPSPLEGLLSAESQAGQEQVA"
     gene            4260..>4451
                     /locus_tag="VI520_06135"
     CDS             4260..>4451
                     /locus_tag="VI520_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37493.1"
                     /translation="MIQITREERHWFLENVARALVEYSGAAEPPVPIEAILDHPPEVF
                     HEDFGVVDMYSNLWDATFAR"
BASE COUNT          874 a         1336 c         1416 g          825 t
ORIGIN      
        1 ccgccgtggg tcgagatgga ggcgggccga aatggaaatc gaattcctcg atccggcgga
       61 ggcgcccttg ccgccggatc aaatgtattt ccgatcggtt gagatcgagc cccattccga
      121 cggccagcgc ctgaaggtct cacttcaggt cgcccccttc caagtgcggc cgtcgatcga
      181 cctcacggtg accgatgacc aggggaaggt cgttgcttcc agcagcattg ttgaggccac
      241 ggagacttcg atgtcggtca ttctgcacct gcgcgatcgg tcctccaagc cgggacatcg
      301 gctgaccgcc tcgctggttt accccgatcg cgagccggta gacgaacgca tactcgaatt
      361 cgcgctccct gatccagatc cgtccgaagc cagctgaggc cgggaatggc gtccagagac
      421 cccctgatcc gccatgccat cgcacaaccc gccttcgatg agcggatcag cttgcatcgg
      481 gaacggctgg ttgacgcgat ccacgccaat atcccccgca agctgattgc aatcgccgcc
      541 cccgccggtt acgggaagac gaccctgttg gccgattttg gctccagcac cgacttgacg
      601 gtgtgttggg cccgcctgac ccaggctgat tgggacatta tgcggtttgc ccaagtgttg
      661 gcggcctcgc tccagagtca cttccgccga ctcaaggggc agccggattt ggccgccctg
      721 gccacggcct cgccagaggc gctggccctc gcctttagtg ttgccatcag cgagaacctc
      781 accgaagcct tcgtcatcgc gctcgatgat atccacctaa tcaaccaatc caagccggtg
      841 ctcgcgttcc ttgatcgctt cctccaggag ctgccggagc aggtcacggt cattgcggcc
      901 ggccgcgagg tgccggaggt ctcgttggcc cgtttgatgg cggaacgcga tctggccgga
      961 ttcggtccgc aggatctggc gttgacccga gaggagttga tcgacctcac ccagagacaa
     1021 cgcggcgccc cgctgagcga tttcgaaatc gaccggctgc tggaagagag ccggggatgg
     1081 gtgaccggga tcgtgatgtc agggacgctg gcggatcgtg gcctgggctc attggtgagt
     1141 tccggacgtc cgatgatgtt cgagtatttg gcatcggtcg tgctcaatca gcagccggac
     1201 gagctgcgcc gcttcgcact ggatgctgca gtactgccga tcatgaccga tgagtcctgc
     1261 aatgtggtct taggccgcga agacagccgc cgactcttga cccaactcgt ccgtaaggcg
     1321 ctgtttgtga cggccacgga aggccccctc cgtacctatg aatttcatcc cctcttcagg
     1381 gagtttcttc tggcctcgct ccaaggggcg gatgtcaagc ggatcgtgga cctgaggaag
     1441 agggccgcca actaccacct cagccgcggg tccttggaac aggcagtcga cttgctgatc
     1501 gaagccggct cggtccgcca ggcggccaaa acggccgagc ggcacgcccg cgagttattc
     1561 gaaagtgggc gggtgcaaac cattgagcgg tgggttgagc ggttccggaa cgaaggcatg
     1621 attgtgccga gtttgaccct ggagttggcc aactcggtca gaaatcgcgg caacctctcc
     1681 ggcgcggagg ccctgctggg ggaggcggag cgggcgatca gccctcgatc ggcgcgtgac
     1741 ctgcaggcgc gattcgagat cgcccgaggt tatctctggt ggcagctcgg caagcacgac
     1801 aaggcgcttg aggcagcaga tcgggtcgac cacctattcg cgaagcgtgc caatcggcat
     1861 tatcaaggaa tcgggaaaaa cattcgggcg ttggccctgg cacacggcag gggcgagttt
     1921 gcgacggctg agcagttgtt ggacgaggca gcccagctgc tcgctcacag cgagaacgag
     1981 ttcggggtct ggacggtgct aatcgatctc ataatggttc aagacgctca gggtaaagcg
     2041 caattggcaa accgaacccg ggagcgggcc ctttcgctgg cgaggaagat aggagccccc
     2101 ttgccgctgg ccgtcgccct cgggaaccgg gctatggggg cccaccagga gggtgaatat
     2161 gagctggcgc ttgaactggg acgcgaggct ctcaaacagg cccgccaggc cgccagcccg
     2221 ttgcgcgagg cgatcagcct gcttcgccag gccgatgtct tcaatgacat tgggctcgcg
     2281 ttgcaggccg ccgagctcta cggtgaggca ctgtcgatcc tgacccgact cgataacgtc
     2341 gaatggatcc gttatgcctg tactcagacc agcgtcctac accgaaggcg cgggggcgcc
     2401 agcctcgccc atgagtggtt gcgaagagcg atgttagcgg atgagagcaa ggttgcccca
     2461 tcggagatcc gggttcagtt cgccgcactc gaaatccagg cggcacccga gcaggcaatc
     2521 aagagcatcc gcagcctgct gaagcggcga gcgacttcgc tcgaggccgg cgaagtgacc
     2581 ctggtgcagt acttccaggc cgtcgccgaa ctcaaactta cgcacccgga gcaggccgtc
     2641 gaattgctga gtcaggcctt tacccaggcc gggcgttccg ggaccgagca agtgatcgcg
     2701 gctgagcttc gcttcgatgc cgcaatgcgg gaattcgcgg tcgggcattt tggcaccgac
     2761 ccgacctatt ccgtgatccg cagtcggatt gagatgatgg aagccctctc ggatcgccac
     2821 gaactgacca ccgaggcgga acccgagggc gttcgaatcg aagtccaggc gctggggtcg
     2881 tcgcgaatcc tggtgggggg aatcgagcct cgggacctga agccactgac tagggagatc
     2941 ttgttctact tgatcgaccg cgaacgggtg aggcgcgaca agctgatgga ggagttctgg
     3001 ccggatcagt cgagcggccg ccaggtgacg agcttgcaca ctgcgattta cggcatccgc
     3061 agctgcatgg gcaaggactc ggtgctcttc gacggaacgg tctaccaaat tgatccatcc
     3121 actcccgtcg agtacgacgt cgcccggttt gaaaaggccg ccctggtcgc ggagcgattg
     3181 ctcccagggg atccgcgctt gctgttcgcg ctgaccgaag cggtccggtc ctatcagggt
     3241 gattttcacc cggaactcga ccgggcctgg gtcgatgaga gacgtagaac cttggaactg
     3301 aagtacctgg acctattggc gagctactca caagaagcat tggtccggga tcgtccctcg
     3361 gaggcgttgg atctcttgcg ccaggccatc cggctcaacc cactccgaga tgacacgaat
     3421 cgttattaca tggaggcact gggacgcctg ggacggcgaa gcgaactagt cgcccattac
     3481 caggactatg tccagctgct tcgccaagag ctcgccctcg atccgccgcc gcatctgacc
     3541 gagctctacg atcgcctgat cagctaggcg attgaggacg gttggaaccg cggaggaagc
     3601 cagcggccgc cttgaggtcc cattcaatgg ggcgagctag tactggggta ccaggtcccc
     3661 ggccagctcg aaacctgacc ggttcagaag tcgctcagag cagtgcagta gagtatagcc
     3721 agagtggaga caaaggtaaa taaaatggtt gccctaaaag gggacggcgg cgtcggagca
     3781 ggttagtcac cggcgcacaa cttaacccgc cgcttagacc gtaagagcgg cggttgggtc
     3841 cctaggcagc gagccaagat gtcctggctg ccgtttttgc gttgaatgaa ggaaaccagg
     3901 aacactactg tgcaaacaag tcaaacaatc ggaaccgaga gctcagcaac ccaatcgaca
     3961 ggaggcaatt ccatgtctat gcaatcgctg aatcagttgg tggcacgctc gatcatcgac
     4021 ccgggtgtgg tccaggcttt ccgtgccgga cgcatcggcg atgtactcac cgaagtgggg
     4081 ttcacccccg atctacaggc ccagctcacc ggaatcgagt gcgactcctg ggccgagttc
     4141 gcggtgatct cataccggca cgtacgggcc gcggaagccc aggcgcctcg cctgcagttc
     4201 ccgtccccgt tggaagggct gttgtcggct gaatcgcagg ccgggcaaga gcaggttgca
     4261 tgatccagat cacccgcgaa gagcgccact ggttcttgga gaacgtggcg cgcgccctgg
     4321 tcgagtatag cggcgccgca gagcctccag tgccgatcga ggccatcctc gatcaccccc
     4381 cagaagtttt ccacgaggat ttcggggtgg tcgacatgta ctcgaacctg tgggatgcga
     4441 cattcgctcg t
//
LOCUS       DATDPQ010000272         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_915906, whole genome shotgun sequence.
ACCESSION   DATDPQ010000272 DATDPQ010000000
VERSION     DATDPQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915906"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VI520_06140"
     CDS             complement(<1..1746)
                     /locus_tag="VI520_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37494.1"
                     /translation="MAGILLFIAFLSLWLASGNAAERLRWRRAYLRASLIWGVFAVAL
                     TEGLSFFNAVAPTTLMVGWGAAAVAASGYIAIRRLRGGGLALPQFSLPPDLGSRMALL
                     VTGAVLAATAAVAWQAPPNTWDSLNYHMSRVAHWEQERGLKHYATGIEVQNSMPPGAE
                     LLVFNLYVLSGGDRLANFVQWFAMVGSLVGVSVLAGQLGAARLGQWTAVVFAATLPMG
                     IVQASSTMTDYVVGIWALAAAVEAVSIWRGEDSPIAASAFFGLAAALGVLAKPTAFAF
                     LAPLALLAIIGLVRARRLAPVLAGLMLIGGLSLALNAGHFSRNIRTYRNPIASTGRIE
                     EHRNAFLGGRGLLSNLLRNAALHAGTPSPHLNKGMALVVLGIHDLIGLDVNDPRTTAT
                     GTFKISTPTTDENRAGNPLQAWLSLPCLIILFAKPQPRKIRWYGLAVLSSFILFSLLF
                     QWQSFGSRYHLPFFLLLSPLVATLLERAARPSLLAGLIGVMFVASWPWLFGIDSRPLL
                     SKRADGGLGSIYTIPREQLYFANAPYLEQPFSEMAALIREGGCTEVGISFAGGGIEYP
                     MWPLLGAPDPDLRVEW"
     gene            complement(1755..2510)
                     /locus_tag="VI520_06145"
     CDS             complement(1755..2510)
                     /locus_tag="VI520_06145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF37495.1"
                     /translation="MASAMKRVALITGASGGIGQAAVQLFAAANWEVVAVDRRAAPRP
                     AEGVRFVQGDVSSSDDVSRVFSELAGQEGRLDTLVNNAAIQISKPILTTSVAEWDDVM
                     ASNLRSAYLTAKHGFDLLRAARGSIVNVSSVHALATSENIAAYAASKGGLLALTRAMA
                     IEFGREGIRVNAVLPGAVDTPMLRESLGRLEGRDLEAKLQALGGRSPLGRVGRPSEIA
                     EAILFLADGERSSFITGQALTVDGGATAQLRTE"
     gene            complement(2544..>3370)
                     /locus_tag="VI520_06150"
     CDS             complement(2544..>3370)
                     /locus_tag="VI520_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLF37496.1"
                     /translation="DGTICGDDSYSAEVLEAAAPRLKVISKWGTGIDSIDSEAAKRLG
                     IQVRNTPGAFTQAVADTVLGYVLAFARGVPWMDRSVKAGEWNKHPGTSLAECTLGVVG
                     VGRIGRAVLERAAAFGMTLLGNDIVRVELGDLPARLTGLNELLAASDFVSLNCDLNAS
                     SRHLINDRTLAQMRSGAVLINTARGSVVDEVALSEALRSGKLAGAALDVFEEEPLPAT
                     SPLRGMDNVLLAPHNSNASPSAWERVHWNTIGNLFEGLGLARPQVKPEEQVAAVRR"
BASE COUNT          664 a         1142 c         1017 g          547 t
ORIGIN      
        1 ccattccact ctcaggtcag gatccggtgc gcccaatagc ggccacatcg gatactctat
       61 cccgcctccg gcaaacgaga tgccaacctc cgtgcatcca ccttcccgaa tgagagctgc
      121 catttcggag aacggctgct cgaggtaggg cgcgttcgca aagtagagct gctcccgcgg
      181 aatcgtgtag atgctgccaa gcccgccatc ggcacgtttc gagagcaggg gacgagagtc
      241 gatgccgaat aaccagggcc aggaggccac gaacatgacc ccgatcagcc cggccagcag
      301 cgacgggcgg gcggctcgtt ccaacagggt cgccaccagc ggcgagagca gcaggaaaaa
      361 cggtaagtga tagcggctgc cgaaactctg ccattggaac agcaggctga agagtatgaa
      421 agacgagaga actgccaagc cataccagcg aatcttcctt ggctgaggct tggcgaacag
      481 gatgatcagg cagggcagcg agagccaggc ctggagcggg ttgccggctc ggttctcatc
      541 ggtcgttggt gtgctgatct tgaaggtgcc ggtcgcggtg gtgcgggggt cattaacatc
      601 caatccgatg aggtcatgga ttcccaagac gaccagcgcc atgcctttgt tgaggtgggg
      661 agagggtgtg ccggcatgca gcgcggcatt gcgcagcaga ttcgacagca ggccccgtcc
      721 tcccaggaat gcgttgcggt gctcttcaat ccggccggtt gaggcaatcg gattgcgata
      781 ggtccgaatg ttccgcgaga agtggccagc attcaacgct agactcagcc ccccgatgag
      841 catgagaccg gccagcaccg gcgcaagccg gcgcgcccga accaacccga tgatcgccaa
      901 tagggccagc ggcgcgagga acgcgaaggc agtcggcttg gcaagcacgc cgagcgctgc
      961 tgccaacccg aagaaggccg aagcggcgat cgggctatct tcaccgcgcc aaatcgaaac
     1021 cgcctcgacc gcagccgcaa gcgcccatat cccgaccaca tagtccgtca tcgtgctgga
     1081 ggcttggaca atgcccatcg ggagggttgc ggcgaagacc actgcggtcc attggccgag
     1141 ccgggccgcg cccagctgcc cggcaagtac ggacaccccg accaggcttc ccaccatcgc
     1201 gaaccactgg acgaaattgg cgagccggtc cccgcctgac agaacataca gattgaagac
     1261 cagcagctcg gcgccgggag gcatgctgtt ttgcacctcg atcccggtcg cataatgctt
     1321 cagaccgcgt tcctgctccc aatgcgccac cctgctcata tggtagttca gcgagtccca
     1381 ggtattgggg ggcgcctgcc aggccaccgc tgccgttgcg gcgagtacgg cgcccgtgac
     1441 gagcagcgcc atccgagagc cgaggtcggg cggaagcgag aactggggca gagccaatcc
     1501 gccgccccgc agtctgcgga tggcgatata tccggaggct gcgaccgcag ccgcccccca
     1561 gcccaccatg agggtcgtgg gagcgactgc gttgaagaag ctcagcccct cggtcagtgc
     1621 gaccgcaaac accccccaga tcagagaggc gcgcagatag gctcgccgcc agcgcagccg
     1681 ttcggccgcg ttgccactcg caagccatag gctgaggaat gcgatgaaca ggaggatgcc
     1741 tgccaacgag gggactactc ggtcctcagt tgagccgtcg cgccgccatc caccgtcagc
     1801 gcttgaccgg tgatgaacga cgaccgttcg ccgtcggcca gaaacaggat cgcctcggcg
     1861 atctcggacg ggcgtccgac ccgacccagc ggcgaccgcc ccccaagggc ctgcagcttc
     1921 gcttcgaggt ccctgccttc aagccggccc aggctctccc tcagcatcgg agtgtcgacc
     1981 gccccgggca gcaccgcatt cacccggatc ccctctcgac cgaactcaat tgccatggct
     2041 cgggtgagtg ccaacaagcc acctttgctg gccgcgtacg ccgcaatatt ttccgaggtg
     2101 gccagggcat ggacggagct cacgttcaca atcgacccgc gagccgctcg gagcaagtcg
     2161 aacccgtgct tggccgtgag gtaggcggag cgcaggttgg aggccatgac gtcgtcccac
     2221 tcggcgaccg aggtggtgag gatcggcttg ctgatctgga tggccgcatt gttgacaagg
     2281 gtatccagcc ttccttcttg gccggcgagc tcactgaaca cccgactcac atcgtcggag
     2341 gacgagacat ccccctgcac gaaccggacc ccttcagccg gacggggcgc ggctctccga
     2401 tcaaccgcca caacctccca attggcagca gcaaagagct ggacggcggc ctgaccgatt
     2461 cctccggagg ccccggtgat caaagctact cgcttcattg ccgaggccat cgcctcacga
     2521 ctccctagcc gtcattacag tctttagcgt ctgaccgccg cgacctgttc ctcgggcttc
     2581 acctgcggac gggccagacc gagcccctcg aaaagattcc cgatggtgtt ccaatgcacc
     2641 cgctcccagg cgcttgggct ggcgttgctg ttgtggggtg ccaagagcac gttgtccatt
     2701 ccgcgcaatg ggctggtcgc aggtaagggt tcctcctcga agacatccag cgcggccccg
     2761 gccaacttcc ccgatcgcag ggcttcggag agcgcgacct catccaccac cgagccgcgg
     2821 gccgtgttta tgagcactgc cccggaacgc atctgggcca aggtgcgatc attgatcagg
     2881 tgcctcgagc tcgcgttcaa gtcgcagttc aggctaacga agtcactggc cgctaacagc
     2941 tcgttcaggc cagtcaaccg cgccggcaga tcgcccagct ctaccctcac aatgtcgttg
     3001 cccaacaggg tcatcccgaa ggctgctgcc cgctccagaa ccgcccgacc gatccgccct
     3061 accccgacca cccccaaggt gcactcggcc agggacgtgc ccggatgctt gttccactcc
     3121 ccggctttca ccgaacgatc catccagggt acgcccctgg caaaggccaa cacataccca
     3181 agcaccgtgt ccgccacggc ttgggtgaag gcccccggtg tgttccgcac ctggatgccg
     3241 aggcgcttgg ccgcctccga atcaatcgag tctattcccg tcccccactt cgagatgact
     3301 ttcaagcgcg gcgcggccgc ttccagcacc tccgcagaat acgagtcgtc cccgcagatc
     3361 gtcccgtcat
//
LOCUS       DATDPQ010000273         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_918196, whole genome shotgun sequence.
ACCESSION   DATDPQ010000273 DATDPQ010000000
VERSION     DATDPQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918196"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VI520_06155"
     CDS             complement(<1..566)
                     /locus_tag="VI520_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HLF37497.1"
                     /translation="MALKGNLRDFSVVQIFNLVNLAHKTGTLTLEGPNVAAWVSFREG
                     KLIHAQLGNEDGTLLGVLTKSGKVRPEQSKLIKKNAADKSDKELGLLLINAGYLSQQD
                     ILASIQHYVLEVVYRLFSWFDGFFRFDNDVLPPDDHITIRLDLENVIIEGSRRIKEVE
                     ALADEIPNLDMAMTFVERPGANIRNVHL"
     gene            complement(669..1211)
                     /locus_tag="VI520_06160"
     CDS             complement(669..1211)
                     /locus_tag="VI520_06160"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HLF37498.1"
                     /translation="MTLSFPTIDWSSLPPALVPALLFGLRAADLTLATLRTLAITRGR
                     PLVSWLLGFTESGLFLLGAVGLLTTLTNPLNLLAYAAGFATGNVIGLSLENRVLPSHA
                     LVRIYSAARGPAIADALRREGWGATEIFGRGFGGSVEMILCFAPRRVANRLRDRVVAL
                     DSEAVVTLQNVRSLLGGWRP"
     gene            complement(1237..1764)
                     /locus_tag="VI520_06165"
     CDS             complement(1237..1764)
                     /locus_tag="VI520_06165"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HLF37499.1"
                     /translation="MLDRFAIPTEWLPLAIFLLRVFDMSFDTLRVLFVVRGRRAPAWF
                     TGFIQSALWVIAVTSVLSHLENLLNLVAYAGGFATGTVVGLFIEERLAIGHGHLRIIS
                     SQRGSAVADALRQAGYGVTELSGRGKDGTVAILTTSVRRRDIDRVRRKVLQADPESFL
                     TVEEVRPLHRGFWRA"
     gene            complement(1853..>2575)
                     /locus_tag="VI520_06170"
     CDS             complement(1853..>2575)
                     /locus_tag="VI520_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLF37500.1"
                     /translation="SPEHLQGYQNIAQLREAELRCAAGHLGLADLHFLGYRDSGMAGS
                     PENQHPEALAGADLQEVTGRITEHIRRIKPQVVITFDPAGGYGHPDHIATHQATVEAF
                     QAAGDSGRFPEAGPPFQPQKLYFSTFSRRFAGLSMALLRMIGHNPRRMGRNQDIDLSE
                     IASQQFPIHARVNYRPVVELKRQATACHASQLDWGPAGRGLAGLIWRLERLGTIDTFM
                     RAHPPASNGPVERDLFQGVREA"
BASE COUNT          511 a          773 c          839 g          452 t
ORIGIN      
        1 ttgaggtgca cgttgcgaat attcgcgccg gggcgctcga caaaggtcat ggccatgtcc
       61 agattgggga tttcgtcggc gagggcctcg acttccttga tccggcggga gccctcgatg
      121 atgacatttt ccaaatcgag ccggatcgtg atgtgatcat ccggaggtag gacatcgtta
      181 tcgaagcgaa aaaacccgtc gaaccaggag aagagccggt atacaacctc cagaacatag
      241 tgttgaatgc tcgccaggat gtcctgttgc gagaggtatc cggcgttgat cagcagcaat
      301 ccgagctctt tgtcggattt gtccgccgcg ttcttcttaa tgagtttgga ttgttcgggg
      361 cgaaccttgc cggacttcgt gagaacgccc agcagggtgc catcttcgtt tcccagctgg
      421 gcgtggatca gtttgccttc tcggaaggac acccaggccg ccacattggg gccttcaagt
      481 gtgagggtcc ctgtcttgtg ggcaaggttg accagattga agatttgcac gacgctgaaa
      541 tcgcgcaggt tgcctttgag cgccattcac ggtctctgga cccaaccccc cagggataga
      601 gtccgccgat tataccctag gggccttgag cgacaaagca ggccattggt atgagcggtc
      661 caacggagct acggacgcca gcccccgagc agcgagcgga cattctggag ggtgaccact
      721 gcctccgaat cgagcgcgac gacccggtcc cgcagccgat tggcaactcg ccgcggggca
      781 aaacacagga tcatttcaac gctcccgccg aatcctcgtc cgaagatctc ggtggcgccc
      841 cagccttctc tccgcagtgc atcggcgatc gccggtccgc gggccgcgct gtagatcctg
      901 accaaggcgt gactcgggag gacccggttc tcgaggctga ggccgatgac gttgccggtc
      961 gcgaacccgg cagcataggc caacaggttg agcgggttgg tcaaggtagt caagagaccc
     1021 accgcaccga gcaggaagag tcccgattcg gtgaagccca gcaaccagga cacgagcgga
     1081 cgcccgcggg tgatggcgag agtccgcaag gtggcaaggg tgaggtcggc cgcccgcagt
     1141 ccgaacagca gtgccggcac cagggccgga ggcaaactgc tccaatcgat cgtgggaaaa
     1201 gagagggtca ttcgcagtcg ccctcggccg cggagctcaa gcccgccaga acccgcggtg
     1261 cagcggccga acctcttcga cggtcaggaa cgactccggg tcagcctgca gaactttccg
     1321 gcggactcga tcgatgtccc gccgtcggac gctggtcgtg aggatggcga ccgtgccgtc
     1381 cttgccgcgt ccggagagtt cggtcacccc gtaaccggct tggcggagcg catccgccac
     1441 cgcgctcccg cgctgggagc tgatgatcct tagatgaccg tggccgatcg ccagccgctc
     1501 ctcgatgaaa agcccgacta cggtgccggt ggcaaagccg ccagcatagg cgacgaggtt
     1561 taggagattc tcaaggtggc tgagcacgct ggtgaccgcg atgacccaca gcgccgactg
     1621 gatgaacccg gtgaaccagg ccggtgcccg ccggccgcgc accacgaaca gtacccgcag
     1681 cgtgtcgaac gacatgtcaa acacccggag cagaaagatc gccaagggca gccactcggt
     1741 gggaatcgcg aatcggtcaa gcatcgcaac agtccggaca ggacggaaaa ggagttctca
     1801 taggtgacct ctacaggcga atgaatccgc ggaagttcga gaggaggcga gtctaagcct
     1861 cgcgaacgcc ttggaacaaa tcccgctcga ccggaccatt gcttgccggc gggtgggccc
     1921 gcatgaatgt gtcgatcgtt ccgaggcgtt ccaggcgcca gatcagcccg gccaggccgc
     1981 gcccggcggg gccccaatcg agctggctgg cgtggcaggc agtggcctgg cgcttcagct
     2041 cgaccaccgg ccggtaattg accctggcat ggattggaaa ctgttggctg gcaatctcgg
     2101 agagatcgat atcttgattg cggcccatcc ggcgcggatt gtggccgatc attctcagca
     2161 aagccatgct gagtccggcg aaacggcgag agaatgtgct gaagtagagc ttctgcggct
     2221 ggaacggagg cccggcctcc gggaatctgc cggagtcgcc ggcggcctgg aaggcctcga
     2281 ctgttgcttg gtgggtggca atatgatcgg ggtggccgta cccgccggcc ggatcaaacg
     2341 tgatgaccac ctgcggcttg atccgccgga tgtgctccgt gattcgccca gttacctcct
     2401 gaaggtccgc ccctgccagt gcctcggggt gttgattctc ggggctgccg gccatgccgg
     2461 aatcacggta gccaagaaag tggaggtcgg caagccctaa gtggccggct gcgcagcgca
     2521 gctcggcctc ccgcagctga gcgatgttct ggtaaccctg cagatgctca ggaga
//
LOCUS       DATDPQ010000274         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2492558, whole genome shotgun sequence.
ACCESSION   DATDPQ010000274 DATDPQ010000000
VERSION     DATDPQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492558"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..649)
                     /locus_tag="VI520_06175"
     CDS             complement(143..649)
                     /locus_tag="VI520_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37501.1"
                     /translation="MRLSAVLPTMVCLAAVVLAGCQSALPDPRPTHSDDTPRPSETRT
                     NPAQTGGPSTDQPQTGGPPAEPSGSIHGPVFIDQAELLIRESFPIQVDLRLSGSLPTP
                     CATLGWSVADPDEQGRIEVEAYSSQDAALACIQVLQEFEETIPLGAYSTGSYSVWLNG
                     ERIGEFEP"
     gene            766..1494
                     /locus_tag="VI520_06180"
     CDS             766..1494
                     /locus_tag="VI520_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HLF37502.1"
                     /translation="MSSGSDRAQFPRREFLTGVRDQLPLLLGVIPFGLIFGALAISVG
                     VSPRAAQGFSLFVFAGSAQFIAVSLIGESASALVVIATIALVNLRHALYSASLAPSLA
                     KLGRTWKLALGWLLTDEAFAMASRRYRNGELEDAHWYTLGTGLTLWAGWQISTAVGIA
                     LGSNLPQGLSLDFALPLTFLALVVPSLIDRPTLAAALAAGLLGLALFNLPFRLGLVAA
                     TIAGVGLGLALESATQPRDEGTLG"
     gene            1487..1807
                     /locus_tag="VI520_06185"
     CDS             1487..1807
                     /locus_tag="VI520_06185"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HLF37503.1"
                     /translation="MAEFTVWLVILGGMIATYLTRLSFILLIPTERVPQFLRRGLKYI
                     PPAVLSAILLPELVILDGSLELAFSNHRLVAGIIAALVAWRLKNTWLTIATGMVALWL
                     LMAR"
     gene            complement(1890..>2553)
                     /locus_tag="VI520_06190"
     CDS             complement(1890..>2553)
                     /locus_tag="VI520_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HLF37504.1"
                     /translation="EHVARVIERMQVWVESHGSDNRLTVLRLKQLDDYCYAVAGIVGE
                     LATSLISLYRPTLERTRLLVLRSLETACGAGLQLTNIIKDVFQDHKEGRYYIPQEYLP
                     FNNHGSLGGLKPIVAHAYRNLCLGRDYVRVVPEEEFDIRKAILVPLLLAVATLRHLLE
                     NLDPLLEGVEVKIPREQVAEILSLADDVAGQNQAVEQAWQDLSGPLLELDGLGLLKAI
                     PA"
BASE COUNT          447 a          793 c          773 g          540 t
ORIGIN      
        1 ggattcgccc gagccgtggc agatcgcatg tttttctttg atgagggagt ggtcgtcgag
       61 agcggcagcc caagcgacat cttcgatcac cccaaggagg aacggaccaa actcttcctc
      121 tctcagatcc tgagccacta attcagggct cgaattcccc gatccgctcg ccattcagcc
      181 atacgctata cgatccggtc gagtaggccc ccagcgggat ggtctcttcg aattcctgga
      241 ggacttgaat gcaagctagc gccgcatctt gcgacgagta ggcctcaact tcgatccggc
      301 cctgctcatc cggatcggca accgaccagc ccagggtggc acacggggtc gggaggctgc
      361 cgctcaagcg cagatcgact tgaatcggga aactctcgcg gatcaacaac tcggcctgat
      421 cgatgaagac cggaccgtga attgacccgc tcggctcggc gggcggccct cccgtctggg
      481 gctggtcagt ggatggacca ccggtctggg ccggattggt ccgagtctct gatggacgcg
      541 gggtgtcgtc cgaatgtgtc ggacgcggat ccggcagcgc gctttgacat cccgcgagca
      601 cgaccgcagc aaggcacacc attgttggca gtacggcaga cagtctcatg gcatcggtcc
      661 tcctggtttg gtcgtaaaga agacgaacag catcgggaga gagttcccag gatagaccga
      721 gtggccggca gtgggaagcc ggcgacggta tgattggccc ccgctatgtc aagtggaagc
      781 gatcgggctc agtttccccg aagggagttc ctcaccggag tacgggatca gcttccgttg
      841 ctgcttggag tgatcccatt cggacttatt ttcggcgcct tggcgatctc ggtgggggtc
      901 agcccgcgtg ccgcccaggg gttttcactt tttgtgttcg cgggcagcgc tcaattcatt
      961 gcggtaagcc tgatcggaga gtcggcttcc gctctggtcg taatcgccac gatcgcgctg
     1021 gtcaatcttc ggcacgcttt gtacagcgcc agcctagcac cctcgctcgc taagctcggc
     1081 cggacctgga agcttgccct cggctggctg ctcaccgacg aagcattcgc aatggcttcg
     1141 cgtcgttacc gcaacgggga gttggaagac gctcactggt ataccctcgg caccggcctg
     1201 acgttatggg ccggctggca gatcagcacc gcggtgggga tcgccttggg atcaaacctc
     1261 cctcaggggt tgtcgctaga ctttgccctg ccattaacct ttttggcttt ggtggtcccg
     1321 agcctgattg accggccgac cctggccgcc gcgctggccg ccggcctgct tgggttggcc
     1381 ctcttcaact tgccgttccg cctcgggttg gtggcggcca caatcgccgg agtcgggctc
     1441 ggtctggcgc ttgaatcagc gacccaaccg cgggacgagg gcacccttgg ctgagtttac
     1501 ggtctggctg gtgatcctcg gcggaatgat cgccacctat ctgacacgcc tctctttcat
     1561 tctattgatc cccacggagc gggtgcctca gttccttcgc cgcggattga aatacatccc
     1621 gcccgcggtg ttgtcggcga tcctcctgcc ggagttggtc atcctggatg gatcgctcga
     1681 acttgccttc tccaaccatc ggctagtcgc cgggatcatc gccgccctgg tggcctggcg
     1741 gctgaagaac acttggttga cgatcgctac cggcatggtg gcactctggc tgctgatggc
     1801 gcggtgaggc cacaggcctg aaacaaaggt gccgtccttc ggacggcgcc tcgacttcgc
     1861 gaatgcgaat ttcctcagtc tagcctagct caagcgggga tcgccttcag cagccccaaa
     1921 ccgtcgagct caagcagggg ccccgagagg tcctgccagg cctgttcgac cgcctggttt
     1981 tgtccggcga cgtcatcggc cagactcagg atctcggcca cctgctccct cggaatcttc
     2041 acctcgacgc cctccagcag gggatccaga ttctcgagta gatggcgcaa ggtggcgacc
     2101 gccagcagca gcggcaccaa gatcgccttg cgaatgtcga actcttcttc cgggactacc
     2161 cgcacgtagt cgcgcccgag gcacaagttt cggtaggcat gggccacgat cggtttcaga
     2221 ccgcccaggc ttccatggtt gttgaacggc aggtactcct gagggatgta atagcggcct
     2281 tccttatggt cttgaaatac atccttgatg atattggtca gctgcaagcc cgccccgcag
     2341 gcagtctcca gcgagcgcag caccagcaag cgggtccgtt ccagggttgg ccgatatagg
     2401 ctgattaggc tggtggccag ttcgcccaca attcccgcca ctgcatagca atagtcgtcc
     2461 agctgcttca ggcggagcac agtcaggcga ttgtcactgc cgtggctttc aacccagacc
     2521 tgcatccgtt cgatcactcg tgcgacgtgt tcg
//
LOCUS       DATDPQ010000275         5014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3299592, whole genome shotgun sequence.
ACCESSION   DATDPQ010000275 DATDPQ010000000
VERSION     DATDPQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299592"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="VI520_06195"
     CDS             complement(<1..1936)
                     /locus_tag="VI520_06195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLF37505.1"
                     /translation="MDPYRLPTSIIPSRYEIRLAPDLEAASFDGQETIALEVREQSEE
                     IALNAAELEVLSAEIADESGRSWPCAVRLDEVTERLHLRPPDALAPGKWQLKLSFRGT
                     LNDRLRGFYRIRTKDPNGQPQTLAATQFESTDARRAFPCWDEPSFKAVFSLSLAIDPE
                     LAAVANTRSLSESIENGRKLVRFADTMVMSSYLVAFAVGELDIPLPTLIGQTPTRVIT
                     LPGKQHLTGFAQAIGEHSLRYFVDYFGLPYPGDKLDHLGLPDFAAGAMENLGLITYRD
                     SALLLDEQASSLTERGYVADVIAHEISHMWFGDLVTMAWWNGTWLNEAFATFMAVKAV
                     DAWRPEWDRWTEFGASRAVALEADGLHHTRPIEFPVESPTDAKAMFDVLTYEKGGSVL
                     RMLEQHIGESTFQEGVREYLRRHAYSNAETHDLWKALGDVAGQKVPDIMHGWVFQPGH
                     PLIDVRRDPSGLHVSQQRFTYLPAEDRPEGAPTPDQQWIVPLQIRLQAAEGSTTVHHL
                     LDRPEATLPAPPDLSSAVVNAGGNGFYRVRYSPDLLAGLLDRLTELTALERLNLVEDN
                     WALTRAGLFPLGDFMELTERFQTETDRQVWTALLGAVSSLRNLIDDDLVKPFAGFVVS
                     RLTPAAERLGLEPRPGDDELT"
     gene            2037..3110
                     /locus_tag="VI520_06200"
     CDS             2037..3110
                     /locus_tag="VI520_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37506.1"
                     /translation="MAYDCELMLHAQRTLAAVLLGSLFMAGCAGVRGNEAPPEAEAPA
                     AAGEAPSPPAVTEIREYPVPAGSHPHDVAPALDGTVWYTAQNSGAVGRLNPISGETRH
                     IPLGSGSAPHGVIVGPDGAAWVTDSGLNAIVRVDPLSEAVTIFPLPSGSGRANLNTAA
                     FDGSGVLWFTGQNGVYGRLDPEQGALEVFDAPGGRGPYGIDATPDGTVYYASLAGSHI
                     ARIDSATGAATVIEPPTPNQGARRVWSDSLGRIWVSEWDAGQLGSYDPATGSWREWLL
                     PGSNPQAYAVYVDELDFVWLSDFGANALVRFDPRTEQFESFPLPSQNAQVRQILGRPG
                     EVWGAESGADRLVVIRFSDSAGS"
     gene            3125..3547
                     /locus_tag="VI520_06205"
     CDS             3125..3547
                     /locus_tag="VI520_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37507.1"
                     /translation="MLNSPQTDAFSGLIILVLLGLGLVILFAVMLLESLVLRRLGWGS
                     IGRCLVDSFVMNLASALVGVILGWVLRIDLYLGRSSGLVGILLLASLALSVIVEAGLI
                     SMVRKREFGETLRPVLRVNVASYALLVVLIAVSNIRRV"
     gene            3908..>5014
                     /locus_tag="VI520_06210"
     CDS             3908..>5014
                     /locus_tag="VI520_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37508.1"
                     /translation="MAAIPWTERAGLRQLFSGRRGRKLRETLTAYLMLAPALTLIFTF
                     GIFPVGFALYVSLHRWLIVRDEFRGIANYVDAVANLAYVGLFAMGLGALVGAGLMLRR
                     VTRQARADQFNPWSYAVPAAFYALTTLAFFRWIFYQLPEVLDIAAKMRGLERTRELFL
                     RLLSEAFHAETVFPVWQQFVGLSLAALAVGLLIRLLRRSPQSATYQANLTIAWLGAVI
                     GFALIGLTFSEIGKVYAAAVETGEDPGIWPQLIVIFSGLLLLGAAWYLWRGAHGELSN
                     RSFAFRLFFAAVLMVGAVLLIVEIPTVVASGDPDLWNGLKVTVYFSLGTVPVQLTIAL
                     FLSVLLFQKVRGASTFRVIYFLPYVTPALASATVF"
BASE COUNT          814 a         1598 c         1634 g          968 t
ORIGIN      
        1 gggtgagctc gtcatccccg ggcctcggtt cgaggccgag acgctcagcg gcaggagtca
       61 gccggctgac cacgaacccg gcgaagggct tgaccagatc gtcgtcgatc aggttgcgca
      121 ggctgctcac cgccccgagc aacgcggtcc acacctgccg atcggtctcg gtctggaagc
      181 gttccgtcaa ctccataaaa tcgccgagcg ggaataggcc ggctcgcgtg agcgcccagt
      241 tgtcctccac taggtttagg cgctccagtg ctgtcaactc agtcaggcgg tccagcagcc
      301 cggcgagcaa gtccggggag tagcggaccc ggtagaaccc gtttccgccg gcgttgacca
      361 cggccgagct caagtcgggt ggggcgggca gggtggcctc cggacggtcg agcaggtggt
      421 ggacggtggt cgaaccctcc gcagcctgaa gccgaatctg gagcggcacg atccattgct
      481 ggtcgggagt gggcgcgccc tccggccggt cttccgccgg caaatacgtg aaccgctgtt
      541 ggctcacgtg cagcccgctt ggatcgcgcc gcacgtcgat caacggatgc cccggctgga
      601 acacccaccc gtgcatgatg tccggcacct tctgcccggc gacatcgccc agggccttcc
      661 acaggtcatg ggtttcggcg ttggagtagg catgccgccg cagatactcc cgcaccccct
      721 cctggaaagt tgattcgccg atgtgctgct cgagcatccg cagcaccgag cctcccttct
      781 cataggtcag tacatcaaac atcgccttgg cgtcggtcgg cgactcgacc ggaaactcga
      841 tcgggcgagt gtgatgcaat ccatccgctt ccagtgccac cgcccgcgag gcgccgaact
      901 cggtccatcg gtcccactca ggccgccagg catcgaccgc cttgaccgcc atgaaggtgg
      961 cgaaggcttc gttcaaccag gtcccgttcc accaggccat cgtgaccagg tcaccgaacc
     1021 acatgtgcga aatctcatgg gcgatcacat cggccacgta tccccgctcg gtcagtgagg
     1081 aggcttgctc gtcgagcagc agcgccgaat cgcggtaggt gatcagaccg aggttttcca
     1141 tcgcaccggc ggcgaaatcc ggaagcccca gatggtcgag cttgtctccc ggatagggca
     1201 ggccgaagta gtccacgaaa tagcgcagcg agtgctcgcc gatggcctga gcgaagccgg
     1261 tcaggtgctg cttcccgggc aaggtgatca cccgggtcgg cgtttgcccg atcagggttg
     1321 gcaaggggat gtcgagctca ccgaccgcga acgcgacgag gtagctggac atcaccattg
     1381 tgtcagcgaa gcgcaccagc ttgcggccgt tctcgatcga ctcggacaag ctgcgggtgt
     1441 tggcaacggc cgccagctcg gggtcgatcg ccagactcag cgagaagacc gctttgaagg
     1501 aaggctcgtc ccagcaaggg aaggcccgcc gggcatcggt ggattcgaac tgagtggcgg
     1561 cgagggtttg aggctggccg ttgggatcct tggtgcggat gcggtagaag ccgcgcagcc
     1621 gatcgttgag tgttccgcgg aacgaaagct ttagctgcca cttgcccgga gccagagcat
     1681 ccggaggtct gaggtggagc cgctcggtga cctcatcgag ccgcacggca catggccagc
     1741 tccggccgct ttcgtctgcg atttccgccg agaggacttc gagctcggcc gcgttcagcg
     1801 cgatctcttc tgactgctcg cgcacttcga gggcgatggt ctcctgccca tcgaagctgg
     1861 ccgcctccag gtccggcgcg aggcggatct cataacggga cgggatgata gaagtgggaa
     1921 ggcggtaggg gtccacgagg tccttggctg cggtggcaga cgattgtacc cgggcgcact
     1981 caaaagcgag tcgggatctg gcttgagcgg ggcggcctcg aagccgacgt caagcattgg
     2041 cgtatgattg tgagctcatg ctgcacgcac aacggacact ggccgcagtc ctacttgggt
     2101 cgctgttcat ggcgggttgc gctggggtcc gtgggaacga ggccccacct gaggccgagg
     2161 cgcccgcggc cgcgggcgag gcgccatcgc ctcccgcggt aaccgaaatc cgggaatacc
     2221 cggtccccgc cggttcgcat ccgcacgacg tggcgcccgc actggatggg acggtttggt
     2281 acaccgccca gaactccggg gccgtgggca gattgaatcc catcagcgga gagacgcggc
     2341 acatcccgct cgggtcaggc tccgcgccgc atggtgtgat cgtcggcccg gacggcgcgg
     2401 cctgggtgac cgacagtgga ctgaacgcga tcgtgcgggt ggacccgcta agcgaggcag
     2461 tgaccatctt cccgctgccc tccgggtcgg gccgggccaa tctcaatacg gcggccttcg
     2521 acgggtcagg cgtgctgtgg ttcaccggtc agaatggcgt ttacggccgg ctggatcccg
     2581 agcagggagc gctcgaagtc tttgatgcgc cgggcgggcg cgggccctac ggaatcgatg
     2641 ctacgccgga cgggacggtc tactacgcct ccctcgccgg cagtcacatc gcccggattg
     2701 attcggcgac tggcgcggcg acggtcatcg agcctcccac gccgaatcag ggagcgcgcc
     2761 gggtgtggtc cgattcgttg ggccgaatct gggtcagtga gtgggacgcc ggccagctcg
     2821 gcagttacga tccggcaacc ggctcctggc gagagtggtt gctccccggc tcgaacccgc
     2881 aagcctacgc ggtgtacgtc gatgaactgg acttcgtctg gctgagcgac tttggagcga
     2941 acgccctggt tcggtttgac ccaagaacgg agcagttcga atcgtttccg ctgcccagcc
     3001 aaaacgccca ggtgcgccag atcctcggtc gcccgggtga agtctgggga gccgagtcgg
     3061 gggccgatag gctggttgtc attcgcttct cggactcggc agggtcgtag ggagattgtc
     3121 cgcgatgctc aactcacccc aaacggacgc ctttagcggg ctcatcattc tcgtcctgct
     3181 tggccttggt ctggtcatcc tattcgcagt gatgctcttg gaatcattgg tattgcggcg
     3241 actgggctgg ggatcgatcg gacggtgtct ggtcgactcg ttcgtcatga accttgcctc
     3301 cgcgctggtc ggcgtgatcc tgggctgggt gttgcgaatc gacctctacc taggtcgatc
     3361 aagcggtctg gtcggcattc tgcttctcgc gtccctggcc ctctcggtga tcgtagaggc
     3421 aggcctgatc tcgatggtgc ggaagcgtga gttcggagag accctgagac cagtgctcag
     3481 ggtcaacgtc gcgagttatg ccctcttggt cgtattgatc gcggtcagca acatccggag
     3541 ggtctgatcg gaagcgacac gtaacccaca tcgcgatgat ggattgaaat cttctggaag
     3601 gcgcgtcctc accatgctgg cactccctga aacggcgctt gctcccgcgc tgatcgggcg
     3661 accctggcct tccccgctgc tccttgccgg cttgctcacc gccggcagtt tcggctgggt
     3721 ttctagcatc gccggactcg cattcctccg gcgcaaacgc tcgccacccg cgaattgagc
     3781 agaactggaa ggtggtatcc tatggccggc ttccgggcaa tgtcggaccg agtcgcgaac
     3841 gcagccgttc cccagccatt gacaatcgaa atcacaccag gccagcttca atctgaagga
     3901 acgccccatg gcggcgattc cctggactga acgggccggg cttcggcaat tgttcagcgg
     3961 ccgccgcggg cggaagctac gcgagacgct caccgcctac ttgatgctcg ccccggccct
     4021 caccctgatc ttcaccttcg gaatcttccc ggtcgggttt gcgctttacg tgagcctgca
     4081 ccgctggctg atcgtccgcg atgaattccg cggcatcgcc aactatgtcg atgcagtcgc
     4141 caacctggcc tacgtagggc tgttcgccat ggggttaggc gctttggtcg gcgccgggct
     4201 gatgctgcgc cgggtcaccc gccaggcccg ggccgatcag ttcaatccgt ggtcgtacgc
     4261 ggttccggcc gccttctatg cgttgaccac cctggccttc ttccgctgga tcttctacca
     4321 gcttccagaa gtcctcgaca ttgcggcaaa gatgcgcggg ctggaacgga ctcgggagct
     4381 gttcctgcgc ttgctgtcgg aagcgttcca cgccgagact gtattcccag tctggcagca
     4441 gttcgtcgga ctctcgttgg ctgccctggc ggtcggcctg cttatccgcc tgctgcgccg
     4501 ctccccccaa tcggccacct atcaggccaa ccttacgatc gcctggctgg gggcggtaat
     4561 cggcttcgcc ctgatcggcc tcacattctc ggagattggc aaggtgtacg cggcggcggt
     4621 cgagaccggg gaggacccgg ggatctggcc gcaattgatc gtgatcttct ccggtttgtt
     4681 gctcctggga gcggcctggt acctgtggcg cggggcacac ggcgaactct ccaatcgaag
     4741 cttcgccttc cggctattct tcgcggcagt gctcatggtc ggcgcggtgc tgctgatcgt
     4801 tgagatcccg accgtcgtgg cttccggcga cccggacttg tggaacgggc tcaaggtcac
     4861 ggtctacttc tcactgggta ccgtgccggt ccagctcacc atcgccctgt tcctctcggt
     4921 cctgcttttc cagaaggtgc ggggggccag caccttccgg gtgatctact tcctgcccta
     4981 cgtcacgccg gctctggcca gcgcgaccgt cttc
//
LOCUS       DATDPQ010000276         3614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3299938, whole genome shotgun sequence.
ACCESSION   DATDPQ010000276 DATDPQ010000000
VERSION     DATDPQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299938"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VI520_06215"
     CDS             <1..433
                     /locus_tag="VI520_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37509.1"
                     /translation="NSGFNGSNTQMRYGQMVGDAFGIDPVFGAMLNPTGGLVGPGNFT
                     LHPGGETPIGYHSVVHDAAGYLHTYHDAGPGYNYLGVEPFPTSFPGTGQVSGIAYWTV
                     QMGGMDPARAIGEAAASTAVSSFRAAVDAGSWVFDKAASIF"
     gene            446..1240
                     /locus_tag="VI520_06220"
     CDS             446..1240
                     /locus_tag="VI520_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37510.1"
                     /translation="MNGIELETEELAYLLATVDAPGLIGVDDPKLVPSTAKSRDTTFS
                     KGRKQLEAHGWVQEISGKPGEYDLNAGLFHTVAVMAAPEMVVATSRGVAEEDRQLVVH
                     YLVREHIVELWATSEKSYFLARLPDRTVLNERMAELLGLAIGAQEGKFMLSESTFNKA
                     KNLAQKEQLAQAEKLLATAGVNGRTGKSLLAALASPVRGQVVVTRANAGEIEAGRRAE
                     LFGEKKGAWVAVRPEADSKELQLSPVGADELAALVRGWLEELGRSA"
     gene            1210..3186
                     /locus_tag="VI520_06225"
     CDS             1210..3186
                     /locus_tag="VI520_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF37511.1"
                     /translation="MAGGTGTFGVSKILRTARLLLLFLLVWVATATATAQTGLTYFIT
                     QVDSSTFPEVHFTLRAVDLGNKAVEGLNPSNLAVYENGEQVSDLEITPQSDGPITYLF
                     VIDQGRASNYQNFGLNNARLAITTLVSGGYFVDGRDTVLVLGRQNVNSDQTVTLLPPT
                     QTATDLSTWAANFNFGRGSGATKGLLGVEDGVRRIQELIPVAGSRTAAILFITRYIED
                     PSATVAPTAAQNTGDAAASNYISVHVLQTDSNQVNKAALEILASASHGQYAAMRSNNY
                     QSVVTSVYQAIAAQRAYYTVTYRSPGADSGQRQITINTPERPSEGAIGSYEITLQPPS
                     ISFIEPTPNSTIRREAEFGADGTSMTFDTSRVRVIAEVGWPDGFARNLESAQLFANGN
                     EEDSLQVAAGQTLLEFEWDVTDNITQGVSSVSLELRLADELGMLAQAELPVNIEVIIP
                     ATPTPAGPRVTPSLVALGIPVLCIAGLALIAAAGGAFYLIRRRASATASASLAEPQGP
                     PVTMMVSDLSPAHALATLTVLEGPKGLVEEVLRITATTTTLGRNPAQSSFAFYADEES
                     SVSRMHCSLSLDDNVFKLTDANSSAGTRLNGRKIQPGVPVILADGDEIVLGDLARRGV
                     KLRFNLATEESRGPYSGTADDRTHLMGDFPGQDS"
     gene            complement(3195..>3614)
                     /locus_tag="VI520_06230"
     CDS             complement(3195..>3614)
                     /locus_tag="VI520_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF37512.1"
                     /translation="STLICERMIEESGTLDAIQRGVREPFQEHFKRVYLTEDHLLTEY
                     DLAWQPAIVGRKDHRNPSNNRLLAVDLEDIEDLPTVSRHHACITLTEGTFFFETIQPR
                     NPTYLDGAQLKPGIRYPIAPGSVIQVGRVSLTFQIVG"
BASE COUNT          719 a         1107 c         1133 g          655 t
ORIGIN      
        1 caactccggc ttcaatggtt caaacactca aatgcgctac ggtcagatgg tgggcgacgc
       61 cttcgggatc gacccggttt tcggcgctat gttgaacccc accggtggcc tggtcgggcc
      121 aggcaatttc accctccacc ctggtggcga gacgccgatc ggctaccaca gcgtggtaca
      181 tgatgctgcc gggtatttgc atacatacca cgatgccggc ccaggctaca actacctggg
      241 agtcgagccc ttccccacct cctttccggg gaccggccag gtctcgggta tcgcctactg
      301 gaccgttcaa atggggggaa tggacccggc tcgggcgatc ggagaagcgg cggcaagcac
      361 agcagtcagc agttttcggg ccgcagtgga cgcaggttcc tgggtattcg acaaagccgc
      421 cagtatcttc taaggacaat cgggcatgaa tggaatagag ctggagacgg aagaactcgc
      481 ctatttgctg gcgacggtgg atgcgcccgg cctgatcggg gtagacgacc cgaaactggt
      541 cccctccacc gccaagtccc gggataccac cttcagcaag ggccgcaagc agctcgaggc
      601 ccacgggtgg gttcaggaga tttcaggcaa gcccggcgag tatgatctga acgcaggtct
      661 cttccacacg gtggcggtca tggctgcccc cgagatggtt gtcgcgacgt cgcgcggggt
      721 ggcggaggaa gatcgacagc tggtagtgca ctacctggtc cgtgagcaca tcgtcgagct
      781 ctgggcgact tctgagaaga gctattttct ggcgcgcctg cccgatcgca ctgtgctgaa
      841 cgagcgaatg gccgagctat tgggtctcgc catcggggcc caagaaggga agttcatgct
      901 gtcagaatct acgttcaaca aggcgaagaa cctcgcccag aaagagcagc tggcgcaggc
      961 cgagaagctg ctggctaccg ccggcgtcaa cggccggacg ggaaaatcgc tgctggcggc
     1021 cctggcgagc ccagtccgcg gccaggtggt ggtaacccgt gccaacgcgg gcgagatcga
     1081 ggccggtcgg cgagccgagc tctttggcga gaaaaagggg gcttgggtgg ccgtccgacc
     1141 ggaggccgat tcgaaagagc tccagctctc ccccgtcggg gccgatgagc tagcggcgct
     1201 ggtgaggggt tggctggagg aactgggacg ttcggcgtga gcaagatcct gcgtactgcg
     1261 cggttgctcc ttttgttcct gctggtttgg gtagctacgg ctacggccac agctcagacc
     1321 ggcctcacgt acttcatcac tcaggtggac tcgagcacct ttcccgaagt ccactttacc
     1381 ctgcgggcgg tggacctggg gaataaggcg gtcgaaggat tgaacccgtc aaacctcgcg
     1441 gtttatgaga acggggagca ggtcagcgat ctggagatca cgcctcaatc cgatggccca
     1501 atcacctacc tcttcgtaat cgatcaaggc cgagcttcga actatcaaaa cttcggattg
     1561 aacaacgccc ggcttgcgat tacgaccctc gtgagcggag gctatttcgt ggatgggcgc
     1621 gatacggtgc tggtgctcgg tcgccagaac gtcaacagcg accagaccgt aaccctgctg
     1681 cctcccactc agactgccac cgacctcagc acctgggccg ccaacttcaa cttcggccgc
     1741 ggaagcggcg cgaccaaggg gctgctcggg gtcgaggacg gcgtccgccg gattcaggaa
     1801 ctgataccgg tggcaggcag ccggaccgcc gcaatcctgt ttatcacgcg ctacattgag
     1861 gaccccagtg ccaccgttgc tcctaccgcg gcccagaaca ccggcgatgc ggcggccagc
     1921 aactacatct cagttcacgt cctgcagacc gactcaaatc aggtcaacaa ggccgcgctg
     1981 gaaattctgg caagcgccag ccatgggcag tatgccgcaa tgcgaagcaa caactaccaa
     2041 agcgttgtca catcggtgta tcaagcgatt gccgcccagc gggcctatta caccgttacc
     2101 taccgcagcc cgggtgccga ttccggccag cgccagatca cgatcaatac ccctgagcgg
     2161 cccagcgaag gcgcgatcgg gtcttacgaa atcacgctcc agcctccgag catttctttc
     2221 atcgaaccga caccgaacag cacgatccgc cgcgaagcgg aattcggggc ggacggcacc
     2281 agcatgacct tcgatacgag ccgggtgcgg gtaattgcgg aagtgggctg gccggacggg
     2341 tttgcccgca atctcgaatc ggcccagcta ttcgccaacg gcaatgagga ggattcactc
     2401 caagtcgcgg ccggccagac cctgctcgag ttcgaatggg acgtcacaga caatatcacc
     2461 cagggcgtca gctcagtatc actggaattg cggctggcgg acgaattagg gatgctcgcc
     2521 caggcggagc taccggtcaa catcgaggtg atcatcccgg ccaccccgac tccagcggga
     2581 ccccgggtga cgccgtcatt ggttgcactg gggattccgg tcctgtgcat tgcaggattg
     2641 gccctgatcg cggcggccgg tggcgccttc tatctgattc gacgccgggc ttctgcgaca
     2701 gcgagcgcct ccttggccga gccgcaggga cccccggtga ccatgatggt ctccgacctg
     2761 tcccccgccc acgccctggc caccctcaca gtattggagg ggccaaaggg gttggtagag
     2821 gaagtcctgc ggattaccgc gactacaacg accctcgggc gcaacccggc ccagagtagt
     2881 ttcgcgttct acgccgacga ggagagctca gtaagccgga tgcactgcag cctctcgctg
     2941 gatgataacg ttttcaagct gacggatgcc aactcgagcg ccgggactcg gctgaacggc
     3001 cgcaagattc agccgggagt tccggtcatc ctggccgatg gcgatgagat cgtgctgggc
     3061 gacctcgccc ggcggggcgt gaagctgcgc ttcaacctcg cgaccgagga gagccggggc
     3121 ccttattcag ggacggccga cgatcggacc cacttgatgg gcgattttcc cggtcaggac
     3181 agctaacctc tcactcagcc gacaatctgg aaggtgaggc tcacccgccc cacctggatc
     3241 accgaacccg gtgcgatcgg gtagcggatc cccggcttga gctgggcccc gtccaggtag
     3301 gtggggtttc tcggctggat cgtctcaaag aagaatgtcc cctcggtgag cgtgatgcag
     3361 gcatggtgtc gagaaactgt cggcaaatct tcgatatcct ccaggtcaac ggccagcagc
     3421 cggttgttgg agggatttcg gtgatccttg cggccgacga tggccggctg ccaggccagg
     3481 tcgtattcgg ttagcaagtg atcttcggta aggtaaaccc gtttgaagtg ctcctgaaac
     3541 ggctcccgaa ctccgcgctg gatcgcatcg agggtaccgc tctcctcaat catccgttca
     3601 cagatcaggg tgga
//
LOCUS       DATDPQ010000277         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2757556, whole genome shotgun sequence.
ACCESSION   DATDPQ010000277 DATDPQ010000000
VERSION     DATDPQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757556"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VI520_06235"
     CDS             <1..417
                     /locus_tag="VI520_06235"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HLF37513.1"
                     /translation="SLTYALMNLANPFLGGIPTCHGSGGVAGHYAFGARTGGSVIIEG
                     GLYVILGLLFGRGFDAVVGVFPLPILGVILLFEGLALMLLVRDLSASKSELTVVLLVG
                     LIAVGLPYGYLIGMIVGVAIAYLLKRGIITLGARES"
     gene            complement(529..810)
                     /locus_tag="VI520_06240"
     CDS             complement(529..810)
                     /locus_tag="VI520_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37514.1"
                     /translation="MLRGIKTIILSVLVAGGIGAIFGVLVGALIGDRPFGGIASGLSS
                     GDLVEALTDPSLIATALLAFLGANFGLALAYGFLPESEADLAEAQPEAG"
     gene            complement(856..1746)
                     /locus_tag="VI520_06245"
     CDS             complement(856..1746)
                     /locus_tag="VI520_06245"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HLF37515.1"
                     /translation="MNPLDNESVLLAFDQPPRRVVSLIPSMTESLYDMGVGDRLVGIT
                     DFCQPKNGTGTLLTRVGGTRSPDIEAVRRLAPDLVIANKEENSRDAVDGLGQVGMTIW
                     LTFPKSTLDALNLLWTLVKLFKLGEQAARVETMARTLDWTDRATTGRLAIPTFCPIWQ
                     GEKSDHGPWFMTFNYQTYAHDVLAKSGGMNVFAGRMRRYPLEADLGLGEAEAAGERDI
                     RYPRVTLEEVRKSAPDVILLPSEPFAFDENHKVLIEGWLADTPAVQAGRVHLIDGSLI
                     TWHGTRMGKALSELPVFFHR"
     gene            complement(1833..2750)
                     /gene="ricT"
                     /locus_tag="VI520_06250"
     CDS             complement(1833..2750)
                     /gene="ricT"
                     /locus_tag="VI520_06250"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HLF37516.1"
                     /translation="MDLSTAERANPGAVAIRFQPLGKLYHFEAGGVADLRPGDFVLVS
                     TTRGREMGEVASLVSRSKAGGGLKPIERRATAQELVERRMWQRKELEAMIDCRAKAAE
                     FGLEGVKIAKAEFSYDGSRLTYLYNYESEDKLDLKPLRDALQEAHPNSRIDFRQIGPR
                     DVAKIIGGMGACGLETRCCSMFLTEFSPISIKMAKAQGISLSPQEITGMCGRLRCCLV
                     YEYEEYVKARKELPRVKKRVMTPLGEGRVVEVLPLKMAVIVLLEKDERRAEFLKHEIE
                     PYDELKALENKANGPCERHQGGGCNCGKG"
     gene            complement(2869..>3105)
                     /locus_tag="VI520_06255"
     CDS             complement(2869..>3105)
                     /locus_tag="VI520_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HLF37517.1"
                     /translation="FGAGGGEKLAQDAQVPFLGSIPIDVRVRKGGDEGMPVVISAPDS
                     EVGKALTSVAEQVAARISVQARTVSNIIPIEMID"
BASE COUNT          557 a          978 c          942 g          628 t
ORIGIN      
        1 agtcttacct acgcccttat gaatcttgcc aatcctttcc ttggcggcat tcccacctgt
       61 cacggatctg gaggggtggc ggggcattat gccttcggcg cacgcaccgg aggttcggtc
      121 atcatcgagg gtgggctcta cgtaatcctg gggctgctgt tcggtcgggg attcgatgca
      181 gtcgttggcg ttttcccgct gcctattcta ggcgtcattc tgttgtttga agggctggcg
      241 ctcatgctgt tggtgcgtga cttgtcagct tccaagagcg agctgactgt tgtcctgctg
      301 gttggtctga ttgcggttgg gcttccctac ggatacctga tcggcatgat cgttggggtc
      361 gccatcgcct atttgttgaa gcgcggcata attacactgg gggcgcggga gtcataacat
      421 cctgtacaac atggcgtcgt gccccggcca caccgaactg gctctcgagc ggctgacgcg
      481 aaaagggggc gggccaatgg tccgcccccc ttcattcaca ccttcagctc agcccgcctc
      541 cggctgcgcc tcggccaggt ccgcctcgct ctcggggagg aaaccgtagg ccagagccag
      601 gccgaagtta gcccccaaaa acgccaggag tgcggtggcg atcagggacg gatcggttag
      661 ggcctcgacc agatcgccgc tagacagccc agaggcgatc cctccgaagg gtcgatcgcc
      721 gatcaacgcg ccgactaaca ctccaaagat cgcgccgatc ccccccgcga ccagcacact
      781 gagaatgatg gttttgattc cgcgaagcat attaagctag attgtaggga gaggggcccg
      841 ccccgtcaag ccgagttagc ggtggaaaaa cactggcaat tcggagagcg ccttacccat
      901 ccgagtgcca tgccaggtga tcagactgcc atcgatcaag tggactcgac ccgcctgcac
      961 cgccggagtg tcggcgagcc agccctcgat cagaaccttg tggttttcat cgaaggcaaa
     1021 gggctcactg gggagcaaga tgacgtccgg ggcggacttt cggacttctt ccaaggtaac
     1081 ccgcgggtaa cggatatcac gctcgccggc cgcttcggcc tccccgagcc cgagatcagc
     1141 ctccagtgga tagcgccgca tccggccggc gaacacgttc atcccgccgg acttggccag
     1201 cacgtcatgg gcataggtct ggtaattgaa cgtcatgaac cacggcccgt ggtcggattt
     1261 ctccccctgc cagatcgggc agaaggtcgg gatggcgagc cgaccggtcg tcgctcgatc
     1321 ggtccaatca agggtgcgag ccatcgtctc gacccgagcg gcctgctccc cgagcttgaa
     1381 gagcttgacc agcgtccaca gtaggttcag ggcatctaga gtgctttttg ggaaagtcag
     1441 ccaaatggtc atgccgacct gcccgaggcc gtcgaccgca tcccggctat tctcttcctt
     1501 gttggcgatc accaggtcgg gggcgagccg ccgaacggcc tcgatgtcag gcgagcgggt
     1561 acccccgact cgggtgagta aggttccagt cccgttcttt ggctgacaga aatcggtgat
     1621 tccgaccagc cggtccccaa cgcccatgtc gtagaggctt tcggtcatgg aggggatcag
     1681 gctgaccacc cggcgaggtg gttggtcgaa cgccaggagc acggattcgt tatccagagg
     1741 gttcatgcac actccaacaa gcaaaccagg ccttagtatt ggcctggact gggctcccgt
     1801 caatgggaac tgggaccgat gggtctggac ctttagccct taccgcagtt gcagcctccg
     1861 ccttggtgcc gttcgcaagg gccgttcgcc ttgttttcga gtgcctttag ttcgtcgtag
     1921 ggctcgatct catgtttcag gaactcggct cgccgctcgt ccttctctaa cagcacgatc
     1981 acggccattt tgagtggcag cacctccacc accctaccct cacccagggg ggtcataacc
     2041 cgtttcttga ctcgcggcag ctctttgcga gcttttacgt actcctcgta ttcgtagacc
     2101 aggcagcagc gcagccggcc gcacatgccg gtgatctctt gcggactgag cgagattccc
     2161 tgggccttcg ccatcttgat cgagattggg ctaaattcgg tcaaaaacat cgagcagcag
     2221 cgagtctcca gtccgcaggc gcccatgccg ccgatgatct tggccacgtc ccgcggcccg
     2281 atctggcgga aatcgatccg tgagtttggg tgggcctcct ggagcgcgtc cctaagcggt
     2341 ttgaggtcca gcttgtcttc ggactcgtag ttgtataggt aagtcagccg agacccatcg
     2401 taggagaatt cggccttggc gatcttcacc ccttccaggc cgaattcggc cgccttggcc
     2461 cggcagtcga tcatcgcttc cagttccttg cgctgccaca ttcgccgctc gaccagctct
     2521 tgggccgtgg cccgccgctc gatcggcttg agcccgccgc cggccttgga gcggctgacc
     2581 agggatgcga cttcccccat ctcacgtccg cgagtggtcg agaccaatac gaagtcgccc
     2641 ggtcgcagat cggcgacccc gccggcttca aagtgataca acttgccgag gggttgaaac
     2701 cggatggcca ccgcgcctgg gttggctcgt tctgccgtgg agagatccat gatgggcaga
     2761 gtatagcatc ggcgccggag acggagaagg gaccctcggt ccgtgcagcc gagcaggcgg
     2821 gcggaccggg catactgggc caccgggtcc cgctcgccgc gggtcgagct agtcgatcat
     2881 ctcgataggg atgatgttcg acacagtgcg ggcctggaca ctgatccggg ccgcgacctg
     2941 ctcggcaacc gaggtcagcg ccttcccgac ttccgaatcc ggggccgaaa tcaccaccgg
     3001 catgccctca tccccgccct tgcggacccg aacgtcgatt gggatcgaac ccaggaaggg
     3061 cacctgggca tcctgagcca gcttctcccc gccgcctgcg ccgaa
//
LOCUS       DATDPQ010000278         3263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2493689, whole genome shotgun sequence.
ACCESSION   DATDPQ010000278 DATDPQ010000000
VERSION     DATDPQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493689"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1402
                     /gene="fabF"
                     /locus_tag="VI520_06260"
     CDS             137..1402
                     /gene="fabF"
                     /locus_tag="VI520_06260"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLF37518.1"
                     /translation="MSRTRVVITGLGAITPLGNTVKETWSALLEGRSGVGPITRFDAS
                     AFLTRIAAEVKGFDPVAELGHKLARRVDRFTQFALVAARQAIEQAKLIINETNRDRIG
                     SIIGTGIGGIGTIVEQMLQFLDRGPKWVSPFTIPMMLPDTAAGQVAIAFGLRGPNMAV
                     VTACASSSNAIGEAAELIRRGDADIMVAGGAEASIVPVAMAGFNVMDAISKRNNHPEG
                     ASRPFDLNRDGFVAAEGSAALVLETYEHAVARGAPILAEVVGYASTNDAYHITAPAAN
                     GSGAAACMRLALADAQLAPEQIEYINAHGTSTKLNDLSETAAIKSVFGEAAYDIPVSS
                     TKSMTGHMLGAAGAVEALFCVKAIEQEVLPPTINYETPDPDCDLDYVPNRKRAKALRF
                     VMSNSFGFGGHNATLILSRFDGNSDVHGT"
     gene            1399..2523
                     /locus_tag="VI520_06265"
     CDS             1399..2523
                     /locus_tag="VI520_06265"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HLF37519.1"
                     /translation="MTRYAHITGWGMAVPERVLTNQDLEKIVNTNDGWIIDMTGIRER
                     RIAGKTDTTASLATQAALRALHGTGVAANEVDLIIVATCSPEHLFPATASIVQDNIGA
                     SQAGAFDLLAACTGFIFALNMAAQAVRSGSIDKALVIGSETLSRLVNYQDRSTAILFG
                     DGAGAFLVEGRDWPGGVLSSVMRSDGSGGELLSIPAGGSYMPTTLDTVRENMHYIQMN
                     GREVFRFATRVMASATREVIEEAGMRVEDISLVIPHQANLRIIEAAARGLKLPLDRFV
                     VNIERYGNTSTASIPIAFCEAWDQGRIRIGDNLVLVGFGAGLTWGALAMTLTERERVQ
                     KVDGRKSTLVVSRARLRSIGRRIGRRLDSLIFRTSSKPPD"
     gene            complement(2595..2969)
                     /gene="trxA"
                     /locus_tag="VI520_06270"
     CDS             complement(2595..2969)
                     /gene="trxA"
                     /locus_tag="VI520_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLF37520.1"
                     /translation="MIVGEPVHVTDEAFEKAVLQSATPVIVDFWAPWCGPCRMVAPVL
                     EKFAAEYQGRLIVAKVNTDDNPQWAGQFDVRGIPTMLFVAGGKVVHRQVGALPEPYLR
                     DMVEEFLGVVEEAASGTNGKER"
     gene            3058..>3263
                     /locus_tag="VI520_06275"
     CDS             3058..>3263
                     /locus_tag="VI520_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37521.1"
                     /translation="MSASATVPGLDCPACTAAGLVYDGQAAFECQYCGTVLAAQRTVC
                     PACSAENLAGADQCFNCNEPLSIVA"
BASE COUNT          617 a         1039 c          999 g          608 t
ORIGIN      
        1 aatctggact agccaatcct cgacataagg gtgtaattct gccttccgag cggggaagcc
       61 ggattttatc atggcgcccg cgccgccttc acaaccacag tgttatactc ctggatcctt
      121 gccgctggag gaattgttga gtaggacgcg ggtcgtaatc accgggctgg gcgccatcac
      181 gcctttggga aacacagtca aggagacctg gtcggcctta ttggagggcc gttccggggt
      241 cggtccaatc acccgcttcg atgcgagcgc gttccttacc cggatcgcgg ccgaggtgaa
      301 gggtttcgat ccagtcgcag agctcggcca caagctggct cgccgggtgg atcggttcac
      361 ccagttcgcg ctcgtcgccg cccgacaggc catcgagcag gcaaaactaa tcatcaacga
      421 aaccaatcgg gatcgaatcg gttcgatcat tggcaccggc atcgggggga tcgggacgat
      481 cgtggagcag atgttgcagt tcctcgatcg gggtccgaag tgggtgagcc cgtttacgat
      541 cccaatgatg ctgccggaca ctgcggccgg gcaggtggca atcgccttcg gcctgcgcgg
      601 cccgaatatg gcagtggtga ccgcctgcgc ctcgagttcg aacgcgattg gggaagcggc
      661 cgagttgatc cggcgcggcg acgcggacat catggtggcc ggcggcgccg aggcgagcat
      721 cgtcccggtg gcgatggcgg gtttcaacgt catggatgcc atctcaaaac gcaacaacca
      781 tccggagggc gcttcacgcc ccttcgacct caatcgggac ggcttcgtcg cagcggaggg
      841 cagcgcagct ttggtgctcg agacctatga gcatgcggtg gcacgcggcg cgccgatcct
      901 ggcagaagtg gtcggatacg cttccaccaa tgacgcctac cacatcactg ccccggccgc
      961 gaacgggtcc ggtgcggcgg cctgcatgcg gctggcgttg gcggatgcgc agctcgcacc
     1021 cgagcagatc gagtacatca atgcccatgg caccagtacc aagctgaacg atctgagtga
     1081 gactgcggcc atcaagagcg tcttcggcga agcggcctat gacatcccgg tctcttcaac
     1141 caagtcgatg accgggcaca tgctgggggc agccggcgcg gtcgaagcct tgttctgcgt
     1201 gaaggcaatt gaacaggaag ttctcccccc gacgatcaac tacgagaccc ccgatccgga
     1261 ctgtgacctg gactacgttc caaaccggaa gcgggcgaaa gcgctgcggt tcgtgatgtc
     1321 aaactccttt ggtttcggcg gccataatgc caccctcatc ctcagtaggt ttgacgggaa
     1381 ctcggatgtg catgggacat gacccgctat gcccacataa cgggctgggg gatggcggtt
     1441 cccgagcgcg ttcttaccaa ccaagatctc gagaagatcg tcaacaccaa cgacggatgg
     1501 atcatcgaca tgaccggcat ccgcgagcgg cggatcgccg gcaagactga cacgaccgcc
     1561 agcctggcca ctcaggccgc gttgagggca ctgcacggca cgggagtcgc cgccaacgaa
     1621 gtcgacctga tcatcgtcgc cacttgctcg cccgagcacc tttttcccgc aaccgcctcc
     1681 atcgtgcaag acaatatcgg cgcaagccag gccggtgctt tcgacctgtt ggccgcctgt
     1741 actggattca tatttgcgct caacatggcc gcccaggccg tccgcagcgg ctcgatcgac
     1801 aaagcgttgg tgatcggatc tgagactctc tcccggctgg tcaactatca agatcgctcg
     1861 accgccatac tgtttggcga cggcgccggt gcgttcctgg tcgaggggcg cgactggccg
     1921 gggggcgtgt tgtccagtgt catgcgctcc gacggctcgg gaggggaact gctctccatc
     1981 ccggccgggg gcagctacat gccgactact ctggataccg tccgggaaaa catgcactac
     2041 atccagatga atggtcggga ggtcttccgg ttcgcgaccc gagtcatggc ctccgccacc
     2101 cgcgaggtca tcgaagaggc cggcatgcga gtggaggaca tctcgctggt gatcccgcac
     2161 caagccaatc tccggatcat cgaggccgcc gcccggggac tcaaactgcc gctcgaccgc
     2221 ttcgtcgtca atatcgagcg ctacggcaac acgtctaccg cttcgatccc catcgcgttt
     2281 tgcgaggcct gggaccaagg gaggattcgc atcggggata atttggttct ggtcggcttc
     2341 ggggccgggt tgacctgggg tgcgctggca atgaccctca ccgagcggga acgggtgcag
     2401 aaagtcgacg ggcgaaagag caccctggtc gtatctcggg ctcggctgag gtcgattggg
     2461 cgccggatcg ggcgccggtt ggacagtctg atctttcgga ccagttccaa gccgcccgac
     2521 tgagcccggg gattagcaga atcaagaaaa caaccgggcg ggttccgccc ggtttcattt
     2581 cctgcgatcg cgcttcagcg ctctttgccg tttgtccccg acgcagcttc ctctactacc
     2641 cccaagaatt cctccaccat gtcccgcaag tacggctccg gcagcgcgcc gacctgccgg
     2701 tggaccactt tcccgccggc gacgaagagc atggtcggga tgccccggac gtcgaactgc
     2761 ccggcccatt ggggattgtc gtccgtgtta actttggcca cgatcaagcg gccctgatac
     2821 tccgccgcaa acttttcgag gaccggggcg accatgcgac aagggccgca ccagggtgcc
     2881 caaaaatcca cgatcaccgg tgtggcggat tgcagcacgg ccttctcaaa cgcctcatcg
     2941 gtcacgtgta ccggttcgcc aacaatcatt tgcctgtaat ccctcccaag tacgtctgaa
     3001 tctatcttat cacgccgaag cactcagccc cccgaccggc ctgtataatc gcggcgaatg
     3061 tcggcctccg ctaccgtacc cggcctcgac tgccccgcct gcaccgccgc cggtttggtt
     3121 tacgacgggc aggccgcctt tgagtgccag tactgcggga ccgtcctggc agcccagcga
     3181 acggtctgtc cagcctgcag cgccgaaaac ctggcaggcg cggaccagtg cttcaactgc
     3241 aacgagccac tttccatcgt tgc
//
LOCUS       DATDPQ010000279         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_659871, whole genome shotgun sequence.
ACCESSION   DATDPQ010000279 DATDPQ010000000
VERSION     DATDPQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659871"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VI520_06280"
     CDS             <1..1015
                     /locus_tag="VI520_06280"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF37522.1"
                     /translation="IEAGCSTFGSSCARSGGSWGCKGFEVRVLVTGGAGFIGSHLVER
                     LLAGGHAVRVLDNLSSGMRADPSAAGYELVEGDVRDRSIVEASLDGVEQVFHLAAMIS
                     VPASTQAPMECYEVNLTGSLQVLDASRQAGVRRVVLASSAAVYGDFLGPVAETTPPAP
                     LSPYAASKLGMEQAARMFTDTFNLPTVCLRFFNVYGPRQAPDSRYAAVIPGFIQAGLV
                     QQPMVIHGDGQQRRDFVFVADAVEACLLAATREQASGGVVNIGGGGSVTIQDLAGILQ
                     RLIPEAPKAILGAVREGDIRYSEAELRRAHALLGYHPATTLEHGLQVTIEWFRHSQAE
                     MPV"
     gene            1012..>2357
                     /locus_tag="VI520_06285"
     CDS             1012..>2357
                     /locus_tag="VI520_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HLF37523.1"
                     /translation="MTRFSRFLQRIASFRPRLPTKAGARAFVDRILAGRPRLSLQGRL
                     LLPKPRLQIRNRVILLGDLILIVTSVLASFALRLDLGVAFSDYLPSTWVMIAIALIVK
                     PAVYYLFGLYRRYWVYASVRELRLIAAATATGSVVVALFVLLARTLGGLQAGFPRSVL
                     GIDWLLSLFSVGGLRLTIRVLAESGQISQKGNGAARQKQVIVVGAGDAGALVVREMQR
                     NPQLNMRPVAFVDDSPDKLKKDIHGVRVVGSLADLERVLRRYLSPEVVMAIPTAPGSV
                     VRLVTEICRVNRVPFRTMPGMHELIGGKVSVSRLRQVDISDLLRREPAKIDDEAVGRA
                     LSGKRVLVTGAGGSIGLELCRQVIRWNPAQLAMLGHGENSIFEALLDLREDGSEVEPI
                     PIIADIRDVDRIQEVFERFRPEVIFHAAAHKHVPMMEINVQEAATNNVLGTQNVVEA"
BASE COUNT          387 a          721 c          783 g          466 t
ORIGIN      
        1 catcgaggct ggctgctcga cctttggatc atcttgcgca cgatcgggcg ggtcgtgggg
       61 ctgcaagggg tttgaagtga gagtcctggt caccggcgga gccggcttta tcggctccca
      121 tctcgtcgaa cggctgctgg cggggggcca cgcggtccgg gtcttggaca atctgagcag
      181 cggtatgcgc gcggacccgt ccgccgccgg ttatgaactc gtcgagggtg acgtgcgcga
      241 tcgatccatt gttgaggcca gcctggacgg ggtcgagcag gtcttccacc tggccgcaat
      301 gatctcagtt ccggcctcga ctcaggctcc gatggaatgt tatgaagtga atttgaccgg
      361 atcgctgcag gtcctggacg cttcgaggca ggccggggtt agacgggtcg tgctggcttc
      421 ttcggccgcg gtctacggtg atttcctcgg gccggtggcg gaaaccacgc ctcccgcgcc
      481 gctctctcct tacgccgcct caaaactagg gatggagcaa gctgcgagga tgttcacgga
      541 tactttcaac ctgccgaccg tctgcctgcg cttcttcaac gtctacggcc ctcgccaggc
      601 gcctgactcc cggtatgccg cggtcatacc cggctttatt caggccgggt tggtccagca
      661 gcccatggtc attcacggcg atgggcagca gcggcgggac ttcgtcttcg tcgccgatgc
      721 cgtggaggcc tgcctgctgg cggccacccg cgagcaagcc agtgggggcg tcgtgaacat
      781 cggcgggggt ggttccgtga ccatccagga cctggcaggc atcttgcagc gactgatccc
      841 ggaggctcca aaggcgatcc tgggagctgt acgggagggg gatatccggt actccgaagc
      901 ggagctgagg cgggcccacg ctttgctcgg ctaccacccc gccacaactc tcgagcatgg
      961 cctgcaagtc acgatagaat ggtttcgcca cagccaggcg gagatgcccg tttgacacgc
     1021 ttctcacggt tcctgcaaag gatcgctagc ttccgacccc gcctacccac caaagcgggc
     1081 gccagagctt tcgttgaccg gatcctggcc ggccggcccc gcttgagcct tcagggccga
     1141 ttactgctcc cgaagccccg cctgcaaatc cgaaaccggg tcattctgct tggagatctg
     1201 atcctgatcg tcacctcggt cttggccagc ttcgccctgc ggctggatct gggagtggct
     1261 ttcagcgatt acttaccgtc gacctgggtg atgattgcca tcgccctgat cgttaagcct
     1321 gcggtctatt acctgtttgg cctttatcgg cgctactggg tctatgccag cgttcgagag
     1381 ctgcgcctga tcgcggccgc caccgcgacc ggctcggtgg tagtcgccct ctttgtgctg
     1441 ctcgcccgca ccctcggcgg actgcaggcc gggttccctc gatcggtctt gggaattgac
     1501 tggctgctct cgttgttcag cgttggcgga ttgaggctga caatccgggt gctggcggag
     1561 agcggacaga tcagccaaaa gggcaacgga gcggcccggc agaagcaggt gatcgtggtg
     1621 ggggccggcg atgccggagc gctggtcgtg cgcgagatgc agcgcaaccc gcagctcaac
     1681 atgcgaccgg tggcgttcgt ggatgacagt ccggacaagc tcaagaaaga cattcacggg
     1741 gttcgggtcg tggggtcgtt ggccgacctg gaacgggtgc tccgccgata tctctcgcca
     1801 gaagtcgtga tggccatccc gaccgccccc ggctcggtgg ttcgcttggt aacggaaatc
     1861 tgccgggtga acagggtccc cttccgaacg atgcccggca tgcacgagct gatcggggga
     1921 aaggtcagcg tcagccggct gcgccaggtg gacatttcag atctgctgcg ccgggaaccg
     1981 gccaagatcg atgacgaggc cgtcggccgg gccctctccg gcaagcgagt cttggtgacc
     2041 ggggctggtg gctcgatcgg gctggagctt tgccggcaag tcattcgctg gaatccggcc
     2101 cagttggcca tgctgggcca cggcgagaac agcatctttg aggccttgct cgacctccgc
     2161 gaagatggtt ccgaggtcga gccgatcccg atcattgccg acattcgcga cgtggaccgg
     2221 attcaagagg tcttcgagcg cttccggccg gaagtcatct tccatgccgc ggcgcacaag
     2281 cacgtgccca tgatggagat caatgttcag gaggcggcga ccaacaacgt gctgggcacc
     2341 cagaacgtgg tggaggc
//
LOCUS       DATDPQ010000280         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3008944, whole genome shotgun sequence.
ACCESSION   DATDPQ010000280 DATDPQ010000000
VERSION     DATDPQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008944"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VI520_06290"
     CDS             <1..617
                     /locus_tag="VI520_06290"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HLF37524.1"
                     /translation="IRSLLRKHLRLAIACGLLVLVIPAGAGAVLEEHDPFCGACHTQP
                     EVEYLERSSAPASDLASSHAEAGTGCIDCHSGIGPTGRVGSLAQGASDLAAYLSGDYP
                     QPSVTALPVGDEGCTKCHRQPSARPDIGSQAAGISSSHYHFAEYTTEWDTRLPQASGT
                     CVACHAAHAQGTSTSQGYRIAAQVNASCEACHRTLSGWVPPIES"
     gene            complement(675..1580)
                     /gene="lipA"
                     /locus_tag="VI520_06295"
     CDS             complement(675..1580)
                     /gene="lipA"
                     /locus_tag="VI520_06295"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HLF37525.1"
                     /translation="MPGRPMRRPEWIKVRAPAGPSYERVRQIMRSKLLHTVCEEARCP
                     NLGECWGAGTATFLLMGDTCTRSCGFCDIATGRPQPLDWLEPERVARAVKAMDLRHAV
                     ITSVNRDEREDGGAPIFAMVIRRIRELQPGCSVEVLIPDFKGSRDALQIVLEARPEIL
                     NHNVETVRRLFKRVQPQDHYEWAEATLSNAKQLDPDVLTKSGIMVGLGETPDEIKETM
                     GDLRTWGVDILTLGQYLQPSVKHLPIERYYHPDEFSELKEYGLGIGFRWVESGPLVRS
                     SYHAEQQVRALSVVHRELYGDGHPQ"
     gene            1894..>2889
                     /locus_tag="VI520_06300"
     CDS             1894..>2889
                     /locus_tag="VI520_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37526.1"
                     /translation="MRRTTSRSWTALVLGLISISVIGSVPRPSLAAQGPDRARPTILE
                     SIEHRSSRPLRELPPIPPRAGELFELPRKLLPNREGSSGPSGLDPLLQPVGDFLAMPA
                     TDQNFEGLNNVNGVLPPDTNGDVGPNHYVQIVNLSFAIWDKSGNLLYGPANTNTLWQA
                     GGGVCATNNNGDPIVLYDHLADRWLISQFALPNFWLLGLGPFYQCIAISQTPDPTGAY
                     HLYTFQISNTKLNDYPKFGVWPDGYYMAMNQFNQLTLSWAGQGVVAFERDKMLQGVTA
                     RGVLFDMYNTDQDLGGLLPADLDGPIPPAGAPNPFVLVDDDGWGYSPDQLQIWNFH"
BASE COUNT          508 a          991 c          866 g          524 t
ORIGIN      
        1 tgattcgctc acttcttagg aaacacttgc ggttggcgat tgcctgcggg ctgctggtgc
       61 tggtgatccc agccggcgca ggcgcggtgc tggaagaaca cgacccgttc tgcggtgcct
      121 gccacaccca gccggaggtg gagtatctcg agcgcagctc ggccccggca tcggatctgg
      181 cctcttccca cgcggaggcg ggcaccggct gcatcgactg ccacagcggc atcgggccga
      241 ccgggcgggt gggctcgctc gcccagggag cgtcggatct tgcagcctat ctctcgggcg
      301 actaccccca gccgtcggtg acggccctcc cggtcgggga tgagggctgc accaagtgcc
      361 accgccagcc cagcgctcgg cccgatatcg gctcgcaggc ggccgggatc tcaagctcgc
      421 actaccactt tgccgaatac accactgaat gggacacccg gctgccgcag gcgagcggca
      481 cctgtgttgc ctgtcatgcg gcccacgccc aaggcacatc caccagccag gggtaccgga
      541 tcgccgccca ggtgaacgcc agctgtgagg cctgccatcg gaccctatcc ggctgggtgc
      601 cgccgatcga gtcgtaacgg aaatgaaaag gcccggtatc cccgggcctg ggttagttcg
      661 cgatctgcat cgccttattg agggtggccg tctccgtaca gctctcgatg cacgaccgag
      721 agcgcccgca cctgctgctc ggcgtgatac gaagaacgca ccagcggccc gctctcgacc
      781 cagcggaaac cgatccccag cccgtactct tttaactcgg agaactcgtc gggatggtag
      841 tagcgctcga tcggcaggtg cttgaccgag ggctgcaggt actggccgag ggtcagaata
      901 tccacgcccc aggtgcgcag gtcgcccatc gtctccttga tctcatccgg ggtctcaccc
      961 agcccgacca tgatcccgga cttggtcaag acatcgggat ccagctgctt ggcgttcgat
     1021 agcgtcgctt cggcccactc gtagtggtcc tgcggctgca ctcgcttgaa cagccgccgc
     1081 acggtctcca cgttgtggtt gaggatctcc gggcgggcct ccaacacaat ctgcagggcg
     1141 tctcgcgatc ccttgaaatc cggaatcaac acctccaccg agcagcccgg ctgcagctcg
     1201 cggatccgcc ggatcaccat ggcgaagatc ggggccccgc catcttcccg ctcgtcccgg
     1261 ttgacgctgg tgatgaccgc gtggcgcagg tccatcgcct tcaccgcccg ggccacccgt
     1321 tctggctcca gccagtcgag cggctgcggc cggccggtcg caatgtcgca aaagccgcag
     1381 gatcgagtac aggtgtcgcc catcagcaag aaagtggccg ttccggcccc ccaacactcg
     1441 cccaggttcg ggcagcgggc ttcctcgcag accgtgtgca gcagcttgct gcgcatgatc
     1501 tgccgcaccc gttcatagct cggcccggcc ggggcgcgca ccttgatcca ctccggacgg
     1561 cgcatagggc gaccgggcag tttccggatt tcggcctcag gtatcagggt tgacacgacc
     1621 tatggatttt accaagccgt ggcccgctcg ctccgagggt ggcttggaac ttcctgggga
     1681 ttgaggccgc ggccacgatc ggataggctt accccgatcg gggacgcttt ccagctgctt
     1741 cagatcgccg gaggagcagc caaccaacca caatcgaaac tcggttcgtg acagacctca
     1801 caatttgctt ttagtgaggc gcaggcccat tgaagaggcc ggtcccaagc tcggatcggt
     1861 tcggttcggt ctcatccagc caggaggaac cccatgcgca ggacaacctc tcgcagttgg
     1921 accgctttgg ttctggggct gatttcgata tcagtgatcg gatcggtccc tcgcccatcc
     1981 ctcgccgccc agggcccaga ccgggcgcgt cccacgatcc tcgaatccat cgagcatcgc
     2041 agctcacgcc cgctgcgtga gctgccgccg atcccgccgc gggccggcga gctgttcgag
     2101 ctcccgcgca agttgctgcc caatcgggag ggcagttcgg gaccctccgg actggaccca
     2161 ctgctccagc cggtgggcga ttttcttgca atgcccgcca ccgaccagaa cttcgaaggc
     2221 ctcaacaacg tcaacggtgt gttgccgccg gacaccaacg gcgatgtcgg ccccaaccac
     2281 tatgtccaga tcgttaacct gtcctttgcc atctgggata agtcaggcaa cctgctgtac
     2341 ggcccggcga acactaacac cctctggcag gcgggcggtg gggtgtgtgc aaccaacaac
     2401 aacggtgacc cgatcgtgct ctacgaccat ctggccgacc gctggctgat cagccagttc
     2461 gcccttccca atttctggct cttgggcctc ggccctttct accaatgcat cgccatctcc
     2521 cagacaccgg acccgaccgg ggcttatcac ctctacacat tccagattag caacaccaaa
     2581 ctgaacgatt accccaagtt cggggtctgg ccggatggct actacatggc catgaaccag
     2641 ttcaaccagc tgaccctgag ctgggccggc cagggtgtgg tggctttcga acgagacaaa
     2701 atgctgcagg gggtcaccgc ccgcggggtt ctcttcgaca tgtacaacac cgatcaggac
     2761 ctgggcgggc tgctgccggc agacctggac gggccgattc ctccggccgg cgcacccaac
     2821 cccttcgtgc tggtcgacga cgatggctgg ggctactcac cggaccagtt gcagatctgg
     2881 aacttccat
//
LOCUS       DATDPQ010000281         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1205155, whole genome shotgun sequence.
ACCESSION   DATDPQ010000281 DATDPQ010000000
VERSION     DATDPQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205155"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VI520_06305"
     CDS             <1..475
                     /locus_tag="VI520_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLF37527.1"
                     /translation="GTLSKAFGVVGGVVAGRQLIVDWLRQRGRPFLFSSAMTVPDVAA
                     CLAAVDLLEESDELVERLWENTRYFKQAMGSLGFDTGASSTPITPIMLGEAPLAQSFS
                     GELFEAGVFAMAIGFPTVAQGKARIRVMISAAHSTDDLDHGLAAFEKVGKRLDVI"
     gene            complement(548..856)
                     /locus_tag="VI520_06310"
     CDS             complement(548..856)
                     /locus_tag="VI520_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37528.1"
                     /translation="MEDSVKLLLTYDPLPKHREEYFRYVLGEFVPALENLGLTLCEAW
                     HTAYGEYPLRLTGFIAEDRETLDTVLASPEFMELESRLQDFVINYSRKIVPQQPRFQF
                     "
     gene            complement(867..2000)
                     /gene="amrS"
                     /locus_tag="VI520_06315"
     CDS             complement(867..2000)
                     /gene="amrS"
                     /locus_tag="VI520_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HLF37529.1"
                     /translation="MRTSTLGDLLDPLTVEGELYETQPDGALRCFACGHRCLIRPGKR
                     GICQVRFNQDGQLRVPWGYVGALQCDPTEKKPFFHLYPGSDTLTFGMLGCDFHCTYCQ
                     NWVTSQAMRDPASDISAAGLREVTPHELVEYGRKMGAWVIGSSYNEPLITSEWAVDVF
                     QVAREAGFTCVYISNGNTTPEVLEFLKPHVTGYKIDLKSMRDKTYRGLGGVLQHVLDS
                     VERVHAMGFWLEVVTLVIPGFNDSSEELWDAARFLAGISPDIPWHVTAFHKDYKLIEP
                     DNTTAEILMRAAEIGQEAGLRYVYAGNLPGQVEDYENTYCPTCRTCLIERLGYVILAY
                     RLTETGSCPSCGTHIPGVWPARAEQVRLGTSEDLYDRRPRPVI"
     gene            2045..>2554
                     /locus_tag="VI520_06320"
     CDS             2045..>2554
                     /locus_tag="VI520_06320"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HLF37530.1"
                     /translation="MITLAVQAGGRSSRMGRDKALIPLGGIPLIEHILRRAAGLAEEV
                     LITTNRSADLAYLGVRLVLDLEPGAGALPGLLTALEAARGETVLLLACDLPFVCRPLL
                     EYLLSLAHEADVVVPQWNGNLEPLHAVYSRSCIETVRQVMQTGQQRVIDLLPRVTQRV
                     VDETEIAQFD"
BASE COUNT          466 a          792 c          808 g          488 t
ORIGIN      
        1 cgggaccctc tcgaaggcct tcggggtggt cggaggcgtg gtggccgggc ggcagctcat
       61 cgtggattgg ctgcgccagc gcgggcggcc gttcctgttc tcctcggcca tgaccgtgcc
      121 agacgtggcg gcctgcctgg cggcagtcga cctgctggag gaatccgacg aactggtgga
      181 acgtctgtgg gaaaacaccc gctacttcaa gcaggcgatg ggttccctag gctttgacac
      241 gggtgcgagc tcgactccga tcaccccgat catgctgggc gaggccccgc tggcgcagtc
      301 cttcagcggc gagctgttcg aggcgggagt cttcgcgatg gcgatcggct tccccaccgt
      361 ggcgcaaggc aaggctcgga tccgagtcat gatctccgcc gcgcattcca ccgacgacct
      421 ggaccatggg ctggccgcgt ttgagaaagt tggcaagcgc ctcgatgtga tctagaaaaa
      481 gatcagccct ggcgggctgg gttgctggct ggctgcgatg agatcgtagt gatcgatggg
      541 ccaggggtca gaactgaaag cgcggttgtt ggggaacgat cttgcggctg tagttaatca
      601 cgaagtcctg cagccggctc tccaactcca tgaactccgg cgaggccagc accgtatcga
      661 gggtctcccg atcttcggca atgaaaccgg tcaggcggag cgggtactcg ccataggccg
      721 tatgccaggc ctcacagagg gtcagcccga gattctcgag cgccgggaca aactcaccca
      781 acacatagcg aaaatactcc tcccgatgtt tcgggagggg gtcgtaggtc aacagcagtt
      841 tgaccgaatc ttccatgctt agtttttcaa atcaccggcc gcggccggcg atcgtataga
      901 tcctcgctcg tccccaatct gacctgctcg gcacgcgccg gccacacccc agggatatgt
      961 gttccgcagc tcgggcagct ccccgtttcg gtcaggcggt aggcgaggat aacatacccc
     1021 agccgctcga ttaggcaagt ccggcacgtc ggacagtagg tgttttcgta gtcctcgacc
     1081 tgtcccggca aattcccggc gtacacgtag cgcaatccgg cttcctgccc gatctcggcg
     1141 gcccgcatca ggatctcggc ggtcgtgttg tccggctcga tcagcttgta gtccttgtgg
     1201 aaggcggtga cgtgccaggg gatgtccggt gagattccgg ccaggaaacg cgccgcatcc
     1261 caaagctcct ccgaactgtc attgaagcca gggatcacca gggtcaccac ctccaaccag
     1321 aagcccatcg catgcactct ctcgacgctg tcgagcacat gctgcagcac gccccccagc
     1381 ccgcggtagg tcttgtcccg catcgacttg aggtcgatct tgtagcccgt gacatgcggc
     1441 ttcaagaact ccaggacctc cggggtggtg ttcccgttgg agatatagac acaggtgaat
     1501 ccggcttccc gcgcgacctg gaacacatcc accgcccatt ccgatgtgat cagcggctcg
     1561 ttgtacgagg agccgatcac ccaggcgccc atcttgcggc cgtattcgac cagctcgtgg
     1621 ggtgtgacct cgcgcaggcc ggccgcagaa atatccgatg ccggatcccg catcgcttga
     1681 gaggtcaccc aattctggca gtaggtgcag tggaagtcgc agcccagcat tccgaaggtc
     1741 agggtatcgg agccggggta gagatggaag aacggcttct tttcggtggg atcgcactgg
     1801 agggcgccga catagcccca ggggactcgc agctgcccgt cctgattgaa gcgaacctgg
     1861 cagattcccc gcttacccgg tcggatcagg cagcggtgac cacaggcgaa acaccgaagc
     1921 gctccgtccg gctgggtctc atacagctcc ccttcgaccg ttaacgggtc gagcagatct
     1981 cccagcgtgc tggtgcgcat tgctcaggtc gggcgattat atcatccgag ggtagaatgc
     2041 ccgcgtgatc accctggccg tgcaggcggg aggccgctcc agccggatgg ggcgcgataa
     2101 ggcgctgatc ccgctcggtg gaatcccgct gatcgagcac atacttcggc gagcagcagg
     2161 ccttgccgaa gaagttctga ttacaaccaa ccggtcggcg gacctggctt acctgggcgt
     2221 ccggctggtg ctcgatcttg agcccggcgc cggcgcgctg cccgggttgt tgaccgcact
     2281 tgaggcggcc cgcggtgaga ccgtcttgct gttagcctgc gacctgccct tcgtctgccg
     2341 gccgctgcta gagtacttgc tcagcctggc gcacgaggcg gatgtggtcg tccctcaatg
     2401 gaacggaaat ctcgagccgc tgcacgcggt gtactcccgc tcatgtatcg aaacggtgcg
     2461 gcaggtcatg caaaccggcc agcagcgggt gatcgacctc ctgccgagag taactcagcg
     2521 ggtcgttgat gaaactgaga tcgcccagtt cgat
//
LOCUS       DATDPQ010000282         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2495307, whole genome shotgun sequence.
ACCESSION   DATDPQ010000282 DATDPQ010000000
VERSION     DATDPQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495307"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VI520_06325"
     CDS             complement(<1..305)
                     /locus_tag="VI520_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37531.1"
                     /translation="MTRGGLVSLLLLLAAACVPAPKVDPSAPRLQKPTVQTSAGAPLS
                     TPTSETRATPVPMPDLGWPAPFTYGNPPAPATPIPPPSPQLGFDRAVMNILLLGSDRR
                     "
     gene            complement(313..1524)
                     /locus_tag="VI520_06330"
     CDS             complement(313..1524)
                     /locus_tag="VI520_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37532.1"
                     /translation="MSEALLAGRLASHLAVAIAFALLFGVATALPPRPANFRWSAAGV
                     PVVGLRLTLVALLLIDSWGIFDTRPLVPWFEWFAAAATALATGWVLLLPQPHPRWDSW
                     LMAVSTLLVVGLSVSVGIEQLAGTSWPVGLWSGFGLLGTGGLRSAEAVFYPVVALLII
                     QKLIRAGVEQPGPGTKPELEASSTSNPEPVEAWAEKFSHRIGGIAEGALGSEPPEQME
                     TLKAELRLALEELAELKSQTERQRPIPLASLTLPIEGRADVISSVETAVAQVAGSLKE
                     RDQSLHLDLAGSLPSVNGDPDELRQILVTLIENAIQASPAGAVIPVSALATDSADGAW
                     VSLSVSDSGGGIPPEDLGRIFHSPEPGRGTESGLARVKQLAEGMGGRVWVGSEVGGGS
                     TVTVLIPARSE"
     gene            complement(1598..>2249)
                     /locus_tag="VI520_06335"
     CDS             complement(1598..>2249)
                     /locus_tag="VI520_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HLF37533.1"
                     /translation="FGEQLSLLAFVGLGLLVLFQLNHLSAAEVLAALILGATALVSAA
                     LLALGMESPDRLTRGLYWAGERLNRLLRPLIRRDLFDLSHAHKLAHDIHEGLQEVRRT
                     PQALLLPAALAVSSKFLLLTVLALVFLAFGQTPFAGTLLASFSVGYLFYIVSPTPSGF
                     GFVEGAMTLVLTSLGVAFGRAAVITLAFRGITFWLSILYGMVAVRWIGLEARPEPQ"
BASE COUNT          457 a          728 c          731 g          333 t
ORIGIN      
        1 cgccgatcgg atccaagcaa gaggatgttc atgactgccc gatcgaagcc caactgcggg
       61 gacggcggtg ggatcggggt tgcaggggcc ggcggattgc cgtaagtgaa cggcgccggc
      121 cacccgaggt ccggcatcgg cacaggagtg gcccgcgttt ccgacgtcgg agtgctcagc
      181 ggcgcaccag cggaggtctg aaccgtcggt ttctgcagcc gcggggcgct ggggtcgact
      241 ttgggagccg gaacgcaggc cgcagccagc aacaggagta gcgaaacaag accgccccga
      301 gtcatcgctg gctcattccg atcgggccgg gatcaatacg gtgaccgtgc tgccccctcc
      361 gacttcgctg ccgacccaga ctcttccccc cattccctcc gccagctgct tgacccgagc
      421 caggccgctc tcggttcctc gccccggctc agggctgtga aaaatccgcc ccaggtcctc
      481 cggaggaatc ccgcccccgg agtcgctgac ggagagactc acccatgcac cgtcggccga
      541 atcggtggcg agtgcagata ccggaatcac cgctccggcc ggggaggcct gaatcgcgtt
      601 ttcgatcagg gtcaccagga tctgacgcaa ttcgtccggg tcgccgttca ccgacggaag
      661 gctcccagct aagtccaggt gaagcgattg atctcgctct ttgagactac cggcgacctg
      721 agcgactgct gtttccaccg agctgatcac gtctgcccgc ccctcgatcg gtaaggtcaa
      781 tgaagccaac ggaataggtc tctgacgctc ggtctggctc ttgagctccg ccagctcctc
      841 cagggcgagc cgcagctccg ccttaagggt ttccatttgc tcggggggct cggagcctag
      901 tgcaccctcg gcaatgccac cgatccggtg actgaacttc tcggcccagg cctcgaccgg
      961 ctcagggttg ctcgtgctgc ttgcttcgag ctcgggcttc gtcccaggtc cgggttgttc
     1021 gacacctgcc cggatcagtt tttggataat gagcagcgcg actaccgggt aaaagaccgc
     1081 ttcggcggac cgcaatccgc cggtgcctag cagaccaaat ccgctccaca gtcctaccgg
     1141 ccaggaggtc cccgccagct gctcaatgcc gaccgacacg ctgagcccga ccacgagcag
     1201 cgtgctgacc gccatcaacc aggagtccca gcgcgggtgc ggctgcggaa ggagcaacac
     1261 ccaaccggtg gccaaggccg ttgccgccgc ggcgaaccac tcaaaccagg ggacgagcgg
     1321 gcgggtgtcg aagatccccc acgaatcgat cagcaggagg gcaaccagcg tcaggcgcag
     1381 gccgaccacc ggcacaccgg cggcggacca gcggaagttg gcaggacgtg gaggaagggc
     1441 ggtcgcgacc ccgaagagca gggcaaaggc gatggcgact gccaggtggg aggccaggcg
     1501 acccgcgagc agcgcctcgg acattggatg gtccgaccga aagtcgggcg gggattatag
     1561 cacgcaggtc agcaggagcg gtcggaggga gacctgccta ctggggttcc gggcgggcct
     1621 caaggccgat ccagcggact gcaaccatgc cgtacaggat ggacaaccag aaggtgattc
     1681 cccggaaggc aagcgtgatc acggccgccc gaccaaacgc taccccgagg gaggtcagca
     1741 ccagggtcat ggccccctct acgaatccga accccgacgg tgtgggcgag acgatataga
     1801 agagatagcc gacgctgaaa ctggcgagca gggtgccggc gaagggagtc tggccgaagg
     1861 ccaggaaaac cagggccagt accgtgagca gcagaaactt gctgctcacc gccagcgccg
     1921 ccgggagcaa gagggcctgc ggcgtacgcc ttacttcctg gagcccctca tggatatcat
     1981 gcgcaagttt gtgcgcgtgg gaaagatcga agagatccct gcgaatgagc ggcctgagaa
     2041 ggcgattcaa ccgctcaccc gcccaataca gaccgcgagt caaccgatct ggggactcca
     2101 ttcccagggc cagcaatgcg gcgctgacca gggcggttgc accaaggatg agcgcggcca
     2161 gcacctccgc tgcgctcaag tgattgagtt gaaaaaggac cagcagaccc agcccgacaa
     2221 atgccaagag gctcagctgc tctccgaag
//
LOCUS       DATDPQ010000283         6551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_927027, whole genome shotgun sequence.
ACCESSION   DATDPQ010000283 DATDPQ010000000
VERSION     DATDPQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927027"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..957)
                     /locus_tag="VI520_06340"
     CDS             complement(22..957)
                     /locus_tag="VI520_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF37534.1"
                     /translation="MTEAIGLLGTTPTPSFAILSYPAQLPSAGLGRPLYETHCASCHG
                     EDGKGVVPGARNFSDLDYMRGETPAFFYTAVTEGRGEMPSFREQLTSDERWDAVFYVW
                     RFSTSAETLALGETIYGDSCAACHGAAGTGEVLGAADFTDLRFTDARAPRDFYLVVTQ
                     GKGSMPAWQGRLSQDERWAVIDYLRAFSYDPALPGDVEVAVPTTVAAEAECPLTDLEQ
                     VNPFGWEDATAVAGGETIYKEACEVCHAADGSGTLPSAPDFSTAEFQSELRSSQGGEL
                     LCVVAEGRGAMPGWKETLTLEQIWQVLTFIASLGK"
     gene            complement(1181..1696)
                     /locus_tag="VI520_06345"
     CDS             complement(1181..1696)
                     /locus_tag="VI520_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF37535.1"
                     /translation="MNPRLRRGLLMIALLLTPFIVGLLFTFEVIKIPFPTDMARQISL
                     SYEEGPRLLPPEGAVPVQGLSGIPEEFPVNPVPADEVSIQRGQILYSLHCQLCHGPQG
                     HGDGELASYFDRTPQNLAGAQTTAEFDGSVYLTIGQGFGQMPSLAENLTPRERWDVIN
                     FVRTLPFSGSE"
     gene            complement(1693..2217)
                     /locus_tag="VI520_06350"
     CDS             complement(1693..2217)
                     /locus_tag="VI520_06350"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HLF37536.1"
                     /translation="MTDTRSLIALFAESTKTAEAVDALHALGIPEDKVTVMTGVPYPE
                     QAIGRHRTWLRLPYIVLAGALGGMLIGLFLAVITPHLYRLDVGGHPPVGFPPAAIIVF
                     VFTMMATIVSTFLGVLWEMDFPRFGPAPYHKSVTDGRLAVLLEFPAGREAEVRQLLQA
                     HQAQDVAEPERMTL"
     gene            complement(2287..3714)
                     /gene="nrfD"
                     /locus_tag="VI520_06355"
     CDS             complement(2287..3714)
                     /gene="nrfD"
                     /locus_tag="VI520_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HLF37537.1"
                     /translation="MSEERRPTEDELPSAAAGEYDERLLLAQRTDAEINQSVLQPMIQ
                     TGRGFWVAVAGLGGLVAWGLFAWGYLVYWGIGVTGKNRPVYWTLFITTFVFWIGISHA
                     GTMISAVLRLLKADWRRPITRGAEAMTAFALMMAALYPLIHLGRVWKFYWMIPYPNNR
                     FMWPNFQSPLMWDLVAISTYLIGSSLYLYLALIPDMAMARDHTDRWRYRLYRGLALGW
                     RGTEAEWHKLHRALGIFSVAIIPIFLSVHSIVSWDFAVTIQPRWHSTIFAPYFVVGAI
                     YSGLAALATILVIVRRAMRLQEFLRAEHFNALAKLVMVAGLFWTYFWFSGFLVEWYGN
                     EPVTRELIQEEVTGRLAPLFYLQMAANLIPIGLLASRKVRTTPALLLAATLLVNVGMF
                     TERLLIVVGSLQRNDLPFNWGMYRPTWVEVSIVVMTFAGFALLYTLFSRIVPYVPVWE
                     IKEGRLRYTLRRIGRLLVPTAAEIE"
     gene            complement(3707..4432)
                     /locus_tag="VI520_06360"
     CDS             complement(3707..4432)
                     /locus_tag="VI520_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLF37538.1"
                     /translation="MARDANPLAESGPRWGLIVDLDRCTGCQACVVACHAENNVPVAG
                     EKQAAYGRWMHWIIIQRYWEGEYPNVKARFMPVMCQQCGEAPCEPVCPVFATYHNQEG
                     LNAQIYNRCIGTRFCGNACPYKVRTFNWFRPEFPEPLTEQLNPDVTVRSRGIMEKCTF
                     CIQRIRRVELDARAEGRQPADGEIQPACVQTCAPGALVFGDLSEPESRASQLARSHRA
                     FRLLEELGTDPGVIYLKGGETSV"
     gene            complement(4404..>6551)
                     /locus_tag="VI520_06365"
     CDS             complement(4404..>6551)
                     /locus_tag="VI520_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLF37539.1"
                     /translation="VSPKLQRILSEPFVRPPEDVLPGRAVWYASTCRQCPAGCGIIVR
                     VINGRAKKIEGNPLHPLNRGKLCARGQAGLQVLYHPDRLRNAVRQTGGRGSRQFEPLY
                     WDEALDLLAGKIRALSDPGRLAVLGGLMPDHLYRLLSLLLESLGAPPPVIFDLHSALE
                     GRSAATHLSEAFFGAPELPVYDLGRADVVFSFGANFLETWMSPVAQSYAYGNMRQGPF
                     GGRGLLVQFEPRLSTTAASADRWIPLRPGTEGFVALALGRIIVEERLGHVGSHRPHAA
                     LYQDVDVTEMAVASQVPIESLRQLARLFADAEHPVAIPGGSLAGQQNGAASMLAVQAL
                     NHVVAQVGRVGGVFLTSPGPTAEFRQAAKVDSFARVQDLIERMKAGEVDLLLVVGANP
                     VFELPAAAGFSSALERIPYVVSFGSFVDETAVRADLILPEHTYLESWGYQVPEPGADR
                     PALSGQQPVVRPVYETRASADVLLALAARLGGRPAEALPWLGEVSFLEETVRSLSGSS
                     LGAYDADTPAGFFALWRQFGGWWSEKELPREPVVLEAAIKPLPIVAPQFVGDEQTYPF
                     LLHPYPSVALSDGRGANLPWLQETPDPMTTARWGTWVELNPEVARSLGVGDDDIVRVI
                     SPHGELEAPVVVYPGIHPEVVAIPVGQGHADYGRFAQQRGSNPLDLVAPVAAADGGAL
                     AWGATRVRLEATGRKQTLARLESLDGAGRESLG"
BASE COUNT         1255 a         2098 c         2137 g         1061 t
ORIGIN      
        1 actggggcga ccgctttcag ctcatttgcc taggctcgca atgaaggtca gcacttgcca
       61 aatctgctcc aaagtgaggg tctctttcca gcccggcatc gcccctcgcc cctcggcaac
      121 cacgcagagc aactcaccgc cttgggaaga acggagctcg ctctggaact ccgcggtgct
      181 gaagtcgggc gcgctgggca gggtgccgga gccgtcggcc gcatggcata cctcgcaggc
      241 ctccttgtag atcgtctcgc cgcctgcgac ggcggtcgca tcctcccagc cgaacggatt
      301 cacttgctcc agatcggtga gaggacactc ggcctcagcc gcgaccgttg tcggtaccgc
      361 gacctccacg tcaccgggca gagcgggatc gtaggaaaag gcgcgcaggt agtcgatgac
      421 cgcccagcgt tcgtcctgcg agaggcgtcc ctgccaggcc ggcatcgagc ctttcccctg
      481 ggtgacgaca aggtaaaagt cgcgcggggc gcgcgcatcg gtgaatcgca gatcggtgaa
      541 atcggcggcg cccagcacct cgccggtgcc tgcagcgcca tggcaggcgg cgcagctgtc
      601 cccgtaaatc gtctcgccca gggcaagcgt ctcagccgag gtggagaagc gccacacata
      661 gaacacggcg tcccatctct cgtcggaagt cagttgctcc ctgaaggagg gcatctcgcc
      721 gcgcccttcg gtgacggcag tgtagaagaa agctggcgtc tcgccccgca tgtagtccaa
      781 atcgctgaag ttccgggcac cgggaacgac tcccttcccg tcctcaccat ggcagctcgc
      841 gcaatgggtc tcatacagcg ggcggccgag accggcagag ggaagctggg ccgggtaact
      901 gaggatcgca aaagaaggcg tgggggttgt ccccagaagc ccaatcgcct cggtcaccgg
      961 gatcagggtg ggaatgggcg ttggccctga aggcaggccg cgaggcgcag cgcaggcggc
     1021 gaagaaggac gccagaacca gcaggagcgg ccatgcgcga cggaagaggc gtccagggtt
     1081 tcggacacct cccggcactg cctcgaggga attcgtggag ggcgttgctc gcccaccccg
     1141 cgcggtgtca gatggcagac agcacatcgc tgatcgctcc tcactcgctc ccggagaagg
     1201 gcagcgtgcg gacgaagttg atgacatccc atcgctcgcg gggtgtcagg ttttcggcca
     1261 gggagggcat ctggccgaag ccttggccga tcgtcaggta gaccgaaccg tcgaactcgg
     1321 cggtggtctg cgctccggcg aggttctgcg gggttcggtc gaagtagctg gccagctctc
     1381 cgtccccgtg gccctgcggc ccatggcaga gctggcagtg gaggctgtag aggatctggc
     1441 cccgctggat tgacacctcg tcagcaggca cgggattcac cggaaactcc tccggaatgc
     1501 ccgagagtcc ctgcaccgga accgccccct caggaggtag cagtcgaggc ccctcttcat
     1561 aagataggga gatttgacgg gccatgtcgg tggggaaagg gatcttaatc acttcaaaag
     1621 tgaagagcag gcccacaata aagggcgtca ggagcaatgc gatcatgaga aggccgcggc
     1681 gaaggcgcgg attcatagcg tcatgcgctc cggctcggcc acatcctggg cctgatgggc
     1741 ctggagcagt tggcgcactt cggcttcgcg ccccgccggg aattcgagca gcaccgccag
     1801 tcggccgtcg gtgaccgact tgtgataggg cgccggcccg aatcggggga agtccatttc
     1861 ccagagcaca cccaggaagg tcgagacgat cgtggccatc atggtgaaaa caaagacgat
     1921 gatggccgcc ggaggaaagc cgaccggcgg atggccgcct acgtccaacc ggtacagatg
     1981 gggcgtgatg accgccagga acaggccgat cagcattccg ccaagcgccc cggccaggac
     2041 gatatacggg aggcgcagcc acgttcgatg gcgtccgatc gcctgctcgg ggtagggcac
     2101 gccggtcatc acggttactt tgtcctccgg gatgcccaag gcgtgcaagg cgtcaaccgc
     2161 ttcggcggtc ttcgtgctct ctgcgaacaa ggcgatcagg cttctcgtat ccgtcatcct
     2221 cgctgatccg catcccatcc tgggccgcga gtttcgagat ggttcgggtc gcggcttccc
     2281 gcaggcctac tcaatctcgg cggccgtcgg caccagcagc cggccgatac gccgaagcgt
     2341 gtagcgcagc cgaccttcct tgatctccca taccgggacg taaggaacga tccgtgagaa
     2401 aagggtgtac agcagcgcga agccggcgaa ggtcatgacc acgatgctca cttcgaccca
     2461 ggtcggccga tacatgcccc agttaaaggg cagatcgttc cgctgcaggc tgcccacgac
     2521 aattagcagt cgttcggtga acatccccac attcaccaac agggtggcag ccaacaggag
     2581 cgcgggagtg gtgcgcacct tccggctggc cagcaggccg atcgggatga gattggcagc
     2641 catctgcaga tagaagagtg gggcgaggcg gccggtgacc tcctcctgga tcaattcgcg
     2701 ggtgacgggt tcgttgccat accactccac cagaaagccc gagaaccaga agtaggtcca
     2761 aaacaagcca gccaccatca ccagcttggc caacgcattg aagtgttcgg cccgtaggaa
     2821 ctcctgcagg cgcatcgccc ggcgcacaat gacgaggatg gtggccagcg ccgccaatcc
     2881 ggagtagatg gcaccgacca caaagtaggg ggcgaagata gtgctgtgcc agcgcggctg
     2941 gatcgtcacc gcgaagtccc acgagacgat cgagtgcacg gacaggaaga tcgggatgat
     3001 ggctaccgaa aagatcccca gggcgcggtg gagcttatgc cactcggctt cggtgccgcg
     3061 ccacccgagc gcaaggccgc ggtagagtcg gtagcgccag cggtcggtgt gatcgcgggc
     3121 catcgccata tcggggatca gcgccaggta aagatacagg ctcgagccga tcaggtaggt
     3181 ggagatggcc accaggtccc acatgagcgg cgactggaaa ttgggccaca tgaagcggtt
     3241 gttcggatac ggaatcatcc aatagaactt ccacacccgg cccaggtgaa tgagcgggta
     3301 gagcgcagcc atcatcaggg cgaaggcggt catggcctcc gcgccgcggg tgatcggccg
     3361 gcgccagtcg gctttgagca ggcgcagcac ggccgaaatc atcgtcccgg cgtggctgat
     3421 gccgatccaa aacacgaagg tggtgatgaa taaggtccag tagactggcc ggttcttgcc
     3481 cgtgacgccg attccccagt aaaccaggta gccccaggcg aacagacccc aggcgaccag
     3541 ccctcccagc cccgccacgg ccacccagaa tccccggccg gtctggatca tcggctgcag
     3601 gacggactga ttgatttccg cgtccgtgcg ctgggcgagc agcagccttt cgtcgtattc
     3661 gcctgctgcc gccgacggca gctcatcctc tgtcggccgc cgttcctcag acactagtct
     3721 ctcctccctt gaggtagatc actcctggat cggtgcccag ttcctccaac agccggaagg
     3781 cccggtggct gcgcgccagt tggctcgccc gcgactccgg ttcgctcaag tctccgaaga
     3841 ccagtgctcc gggggcacag gtctggacgc aggccggttg aatctccccg tcggcgggct
     3901 ggcggccctc cgctcgggca tcgagttcaa cccgtcggat gcgctggatg cagaaagtgc
     3961 acttctccat gatgccgcgc gaacgtaccg tgacgtccgg gttgagctgc tcggtaagcg
     4021 gttcggggaa ctctgggcgg aaccaattga aggtgcggac cttgtagggg caggcgttcc
     4081 cgcagaagcg ggtgccgatg cagcggttgt aaatctgcgc gttcagccct tcctggttgt
     4141 ggtaagtagc aaatacggga catacgggct cgcacggcgc ctcgccgcac tgctggcaca
     4201 tgaccggcat gaagcgcgcc ttgacattgg ggtattcgcc ttcccaatag cgctggatga
     4261 tgatccagtg catccagcgg ccgtaggcgg cttgcttctc gcccgcaacc ggcacattgt
     4321 tctcggcatg gcaggccaca acgcaagcct ggcagcccgt gcagcggtcc aggtcaacga
     4381 tcagtcccca gcgaggcccg ctctcagcca agggattcgc gtcccgcgcc atccagactc
     4441 tccaatcgtg ccagggtctg cttccggcca gtggcctcca gccgcacccg ggttgccccc
     4501 cacgccaatg ccccgccgtc agccgcggca accggtgcta ccaggtcgag tggattcgac
     4561 ccacgctgct gggcaaaccg gccatagtcg gcgtgcccct gacccacggg aatggcgact
     4621 acctccgggt gaatccctgg gtagacgacc accggcgctt cgagttcccc gtgcggagag
     4681 atcacccgga cgatgtcgtc gtctcctacg ccgagcgatc gggcgacctc cgggttaagt
     4741 tcgacccagg taccccagcg ggcagtggtc atcgggtccg gggtctcttg cagccagggc
     4801 aagttcgcac cgcggccatc ggaaagtgcg accgacggat agggatggag gaggaagggg
     4861 taggtttgct cgtctcccac aaactggggc gcgacgattg gcaggggctt gatagctgcc
     4921 tcaagcacta ccggctcgcg tggcaactcc ttctccgacc accagccgcc gaattgcctc
     4981 cagagcgcaa agaaacctgc cggagtgtcg gcatcgtagg ctcccaggct ggagccggat
     5041 aggctgcgga cggtttcttc caagaatgag acctcgccca gccaggggag cgcctcggcc
     5101 ggccggccgc ccagtcgggc ggccagggcg agcaatacat ccgcgctggc gcgtgtttcg
     5161 tacaccggac gcacgactgg ctgctgaccg cttaacgccg gccgatcggc tcccggttcc
     5221 ggcacttggt atccccacga ttccaggtac gtatgctcgg gcaggatcag gtcggcgcgc
     5281 acggcggttt cgtctacaaa tgagccgaaa gaaacgacat acggtatacg ctcgagggcc
     5341 gagctgaacc ccgccgcggc cgggagctcg aagaccgggt tggcaccaac caccaacaag
     5401 agatcgacct cgccggcctt catccgttcg atcaggtcct gcacccgggc gaaagagtcc
     5461 acctttgccg cctggcggaa ctcggcggtt ggccccgggg aagtgagaaa cactccgccc
     5521 acccggccca cctgagccac gacatgattc agcgcttgca ccgccagcat gctcgctgcg
     5581 ccattttgtt gtccggccaa cgaccctccc gggatggcga ccgggtgctc agcatcggcg
     5641 aatagccggg ccagttgacg caggctctcg atcggcacct ggctggcgac cgccatttca
     5701 gtcacatcca catcctgata cagcgccgcg tgagggcgat ggctgccgac gtgcccgagt
     5761 cgttcctcaa caatgatccg gccgagggcc agggcgacaa aaccttcggt gccaggccgc
     5821 agagggatcc atcggtccgc ggaggccgcc gtggtggaaa ggcgtggctc gaactggacc
     5881 agcaagccgc ggccgccgaa gggcccctgg cgcatattcc cgtaggcata actctgggcc
     5941 actggggaca tccaggtttc gaggaagttc gccccgaagg agaaaacgac gtcggcccgg
     6001 cccaggtcat agaccggcag ctcgggcgcc ccgaagaagg cctcagacag gtgtgtggcg
     6061 gccgaccggc cttcgagagc cgagtggagg tcgaatatca ccgggggagg tgcacccagc
     6121 gactccagca ggagggagag cagccggtaa aggtggtcag gcatcagacc tcccaggacc
     6181 gcgaggcggc ccgggtcgct cagggcccgg atcttcccgg ccagaagatc cagtgcctcg
     6241 tcccagtaca ggggttcaaa ctggcgcgag ccgcggcccc cggtctgacg gaccgcgttc
     6301 ctcagtcggt cgggatggta gagcacttgt aggcccgcct gtcctcgggc gcacaacttc
     6361 ccccggttta aggggtggag tgggttgccc tcgatcttct tggcgcggcc attgatcact
     6421 cggacgatga tcccgcatcc ggccgggcat tggcggcagg tgctggcgta ccaaaccgcc
     6481 cgacccggga gcacgtcctc cggcggacga acgaagggct cggagaggat ccgctgcagt
     6541 ttaggggaaa c
//
LOCUS       DATDPQ010000284         2978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_667269, whole genome shotgun sequence.
ACCESSION   DATDPQ010000284 DATDPQ010000000
VERSION     DATDPQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667269"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VI520_06370"
     CDS             <1..361
                     /locus_tag="VI520_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37540.1"
                     /translation="APIAGPADGAIDLRPAQVLVATAGSAAGRLAKLNPIPMLTAALE
                     AVGLGGEGFGFVLRYGRYAPEGRKSQFLVGGLGLADLSKRLTGWLEAQGPLEGQVQVI
                     DEGSGELTIYLLLPEAA"
     gene            396..>2978
                     /locus_tag="VI520_06375"
     CDS             396..>2978
                     /locus_tag="VI520_06375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF37541.1"
                     /translation="MTSLKARIKDLGVWRASGAVFCLILLGLAVAFPRLVLPVLALSC
                     GSLFFLYGLKSYASIAVIMLSTSGANGNGNGHDGSRKGLVNRLRKANSHHPNGGHGSG
                     KLFKLPADKQPFISIQLPMYNEDRVVDRLLSACTQLDYENYEVLVADDSSDQTLHSLE
                     RWAKHPKVRVSHRINRSGFKGAALKHASEVMNPKAQFVAVFDADFIPPPDILHQFLSY
                     FYGVGGSNGNELNLIDESLAVVQGYQWHVLNASENWITRAIRAEFAGSYVIERSTQEL
                     TGGMKMISGSVFMIRADVVRKHKWGTSITEDWELTLKLYLDGYKVLYTPFIQAPAECV
                     SDFKQLTRQRMRWAEGHTFNVKRFFLPVMRSPKLSLREKLEFIYYAPYYLTSVLFMLG
                     TTAWMISELFLRVQLPYWSEVFGWSLVLTNSAALILMNLAGLFQERGVRRSWMGLFSF
                     ALLTFLLVPYQAYAALKGLIEPHEGGWNRTQKSGVVTDVIKRMGLGRRMPKLLPKKKK
                     SGFDLGKRLPAVARLSRWVPQPVRTLATKSSRSARLALALAGAIAILALLVRSVSVVS
                     AAPASYFLHDADLGPSVSPAGKITNTTLGSGAATMVFDTAGQNAYWYSDQTYPTGAGD
                     ASIAAGTYTLNMYFASLPGNLTQVSDSFTEAGSTILTSHTPDTGTGWTKVFDSSTATT
                     DATIDAALDITRAGSEENNKGQAYTAAPAPTGAAQMISFELTALITETGTTPVGVFAR
                     RTDNSNFYYLQILPNGHAEDSLKLFKFVGGVATELATVDATLSAEDTFRLELTDATKK
                     VFHNAVEVLSSTDNALTAAGTWGLFYGNFNGTGGHLGMGWNVDDFLAQEPSDVQITVT
                     VYHTR"
BASE COUNT          574 a          932 c          897 g          575 t
ORIGIN      
        1 ggctccgatt gccgggcccg cagacggtgc catcgacctg cgccccgcgc aggttctggt
       61 agccactgca ggatccgcag ctggacgact ggccaagttg aacccgatcc cgatgctgac
      121 tgccgccctc gaggcggttg ggctgggcgg ggaggggttc ggctttgtcc tgcgctacgg
      181 ccgatacgct cccgaaggca ggaagtctca gttcttggtc gggggcctgg gtctggcgga
      241 cctgtcgaaa cggctgacgg gctggctaga ggcgcagggt cctttagagg gtcaggtgca
      301 agtcatcgac gagggttccg gcgaattgac gatctatctg ctcctcccgg aggcggcgtg
      361 aagcggcctg ccgagttgag caaagtgcca aggcaatgac gagcctgaag gcgcgcatta
      421 aggatctggg ggtgtggcgg gcgagcgggg cagtgttctg cctcatcctg ctcggattgg
      481 cagtggcctt cccgcgactt gtgctcccgg ttctcgcttt gtcgtgcggc agcctcttct
      541 tcctctacgg gctgaagtcc tatgccagta tcgctgtgat catgctgtcc acctcgggag
      601 cgaatggcaa cggcaatggc catgacggca gccgcaaggg cctcgtgaac cgtctgcgaa
      661 aggcaaacag ccaccatccc aacggcggcc atgggagcgg gaaactcttc aagctcccgg
      721 ccgacaagca gcccttcatt tcgatccaac tcccgatgta caacgaagat cgggtcgtcg
      781 atcgtctcct gtcggcctgc acccagctcg attatgagaa ctacgaggtg ctggtggccg
      841 atgactcgag cgatcaaact ctgcacagcc ttgaacgctg ggcgaagcac ccgaaagtaa
      901 gggtgtcgca tcgcatcaac cggagtgggt tcaagggggc tgcgctcaaa catgcctcgg
      961 aggtgatgaa tcccaaggca cagtttgtcg cagtgttcga tgcggacttc atccccccgc
     1021 ccgacatcct gcatcaattc ctctcgtact tctacggggt cggtggctcc aacgggaacg
     1081 agctcaacct gatcgacgag tcgctagccg ttgtgcaggg atatcagtgg cacgtgctca
     1141 acgcctccga gaactggatc acccgcgcca tccgggctga attcgccggc tcctatgtga
     1201 tcgagcgctc cacccaggag ctgaccggcg gtatgaagat gatttccggc tcggtcttca
     1261 tgatccgggc cgacgtcgtg cgcaagcaca agtggggcac ctcgattacc gaggactggg
     1321 agctgacgct aaagctgtac ctggatggct acaaggtgct ctacaccccc ttcatccaag
     1381 cgccagccga atgcgtttcc gatttcaagc aactgacgcg ccagcggatg cgctgggccg
     1441 agggccacac cttcaacgtc aagcgcttct tcctgccggt catgcggtcg cccaagctgt
     1501 ccctgcggga gaagttggaa ttcatctatt acgcgcccta ttacctgact tcggttctgt
     1561 tcatgctagg aaccacggcc tggatgattt cggagctgtt cctgcgggtc cagttgccct
     1621 actggtcgga agtctttggc tggtcgctgg tcctcaccaa ctctgccgcc ctaatcctca
     1681 tgaacctggc cggtctattc caggagcgag gagtccggcg cagctggatg ggacttttct
     1741 cctttgccct gctgaccttc ctgctggttc cgtatcaggc ctatgcggcg ctgaaagggc
     1801 tgatcgaacc ccacgagggc ggctggaacc gcacccagaa gtctggggtg gtgaccgatg
     1861 tgatcaaacg catggggctc ggccggcgga tgcccaagct gctgccgaag aaaaagaaga
     1921 gcgggttcga tcttgggaag cggctgcccg cggtcgccag gctttcccgc tgggtgcctc
     1981 agccggtccg aaccctggcc accaaatcca gccggtccgc ccgcctggcg ttggccttgg
     2041 ccggcgcgat cgcgattctg gccctgctgg tgcgcagcgt aagcgtcgtc tcggccgcac
     2101 cggcttccta ctttctacac gatgccgact tgggacctag cgtcagtcct gccggaaaga
     2161 tcacaaacac cacgctcgga tccggtgcgg cgacgatggt cttcgacacg gctgggcaaa
     2221 atgcctattg gtacagcgac caaacgtatc ccaccggcgc aggtgacgct agcatcgctg
     2281 ccgggaccta taccctgaac atgtacttcg cctcactccc gggcaacctg acccaggtga
     2341 gcgacagttt caccgaagcc ggcagcacca tcctcacctc ccacacccct gacaccggca
     2401 ccggctggac gaaggtgttc gacagcagca ccgctactac ggacgccacg attgatgctg
     2461 ccctggatat cacaagggca ggttcggagg agaacaacaa ggggcaggcc tacacggcag
     2521 ccccagctcc gaccggcgcc gcccagatga tcagcttcga gttgacggcc ctcatcaccg
     2581 aaaccggaac cacgcccgtc ggggtcttcg cccggcggac cgacaacagc aacttctatt
     2641 acctccagat ccttcccaac ggacatgccg aggacagcct gaagctgttc aagttcgtgg
     2701 gaggggtagc gaccgagttg gccactgtag acgcgaccct ttccgccgag gacacgttca
     2761 gactcgagct cacggatgcc acaaagaaag tcttccacaa cgccgtcgag gtcctcagct
     2821 cgaccgacaa tgcactcact gccgctggaa cctgggggct cttttatggc aatttcaatg
     2881 ggacaggggg ccaccttggg atggggtgga atgtagatga tttcctggcc caagagccgt
     2941 ccgatgttca gatcacagtc acggtctacc acacgcgg
//
LOCUS       DATDPQ010000285         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_670912, whole genome shotgun sequence.
ACCESSION   DATDPQ010000285 DATDPQ010000000
VERSION     DATDPQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670912"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VI520_06380"
     CDS             <1..807
                     /locus_tag="VI520_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLF37542.1"
                     /translation="GRTVRIVGVDPIGSLLYDAWRAGGSAVGLEAITYKVEGIGEDFV
                     PSTLDLGLVDDVVQVDDAEAFLWTRRLVREEGIFCGGSSGAAMAGAVKAARDLGPERV
                     MVVIFPDSGSRYLSKVFSDDWMREHGFLEPERRLITVGEVSRARGLPSLVTASPDDTL
                     LDVIGRMRKHAVSQIPVVDGEGRLIGLVTELMLLDYMLAADPVRLPAMNIGPVVNTEV
                     ATASASDPLEEALPKLVASKVVILVDPARMPTGILTVIDALDYMAAREIR"
     gene            804..1637
                     /locus_tag="VI520_06385"
     CDS             804..1637
                     /locus_tag="VI520_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HLF37543.1"
                     /translation="MNQLPELPELQIVPTESLQPHEEIEARRAQRLIRSLQTEGFLKN
                     PPVVLQIRQDPDRYVVLDGANRTTAFKQMGFPHILVQIARSGVEVMSWNHVLRGENSA
                     PLLEAIRGLADARLTPTDPQRAPAALQTRQSLMYLVVPGGEVWQLESPELQLGARVEL
                     LGKVVKLSERGFRRERTGAEDLNGLVKLYPDLSCLIVYPRFEVRDVIEVAASGGLFPS
                     GTTRFLISPRALRVNFPLATLADRSPLESKRQVLDEWIRERVEQRRVRYYAESTFLFD
                     E"
     gene            complement(1638..2783)
                     /locus_tag="VI520_06390"
     CDS             complement(1638..2783)
                     /locus_tag="VI520_06390"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HLF37544.1"
                     /translation="MPEFETLIIHAGQPPDPTTGAIMTPIYQTSTYRQEGVGRHRGYE
                     YSRTGNPTRTALERCLAALEDGTNGLAFASGMAAIDAIAHLLQPGDHVLAVDDLYGGT
                     YRLFETVYAKLGISFSYASPANPAEFVSQARAETRLIWLETPTNPLLRVCDIAEIARL
                     AKRSRPGVLVAVDNTFATPYLQRPLQLGADLVHHSTTKYLSGHSDVVGGAVICRDEQT
                     AGRLRVLQNSIGAVPGPFDCFLVLRGIKTLGVRMDRHCSSAQLVAEHLKRMKGVAVVY
                     YPGLAEPPQAELANRQMRKPGGMVSFVLRAGAAAAVRFAEATQLFSLAESLGSVESLI
                     EVPAAMTHASTAGTPLAIDPSLIRLSVGLEAVGDLIADLDQAMDSSS"
BASE COUNT          552 a          853 c          959 g          547 t
ORIGIN      
        1 ggacgcacgg ttcggattgt aggagtcgac ccgatcggtt cgctgctcta cgacgcgtgg
       61 cgggcgggcg ggagtgcggt cgggctggaa gcgatcactt acaaagtcga agggataggc
      121 gaggattttg tcccctcgac cttggacttg gggctagtgg acgacgtagt tcaggtggac
      181 gacgccgagg ctttcctgtg gacccgaagg cttgtccggg aggaagggat cttctgcggc
      241 ggctcctccg gggcggcgat ggcgggggcg gtcaaggccg ccagggatct cggacccgag
      301 cgggtgatgg tggtgatctt tccggactcc gggtcgcgct acctatccaa ggtcttcagc
      361 gacgactgga tgcgagaaca cgggttcctg gagcccgagc gccggctcat aacggttggg
      421 gaggtcagcc gagcgcgcgg ccttccctcg ttggtcactg cctccccgga tgatacgttg
      481 cttgatgtga tcggtcgcat gcgcaagcac gcggtgtcac agataccggt ggtggatggg
      541 gaaggacgcc tgattggcct ggtcaccgaa ctcatgctac tggattacat gctggctgcc
      601 gatcccgttc ggctgcctgc aatgaatatc gggcccgtgg taaataccga ggtggccacg
      661 gcatccgcct cagacccgct ggaagaggcc ctgcccaagt tggttgccag caaagtggtg
      721 atcttggtcg accccgctcg tatgccgacc gggatcctca cggtcatcga cgccctcgac
      781 tacatggctg cccgagagat ccggtgaatc agctccctga actgccggaa ctgcagatcg
      841 ttccgaccga gtcgcttcag ccccacgaag aaatcgaggc gcggcgggca cagcgtttga
      901 ttcgctcgct ccagaccgag ggattcctga agaacccccc ggttgttctg cagatcaggc
      961 aggacccgga ccgctacgtc gtgctggacg gagcaaatcg gaccacggct ttcaaacaaa
     1021 tgggcttccc tcacatcttg gtccagatcg ccaggagcgg ggtggaagtc atgagttgga
     1081 atcacgtctt acgcggcgag aactcggcgc ccttgctgga agccatacga ggcctcgcgg
     1141 atgcccggtt gacgccgact gacccgcagc gagctccggc cgccttacaa accaggcagt
     1201 cgctcatgta cctggtggtg ccggggggag aggtgtggca attggagagc cccgagctgc
     1261 agctcggggc gcgggttgag ctgcttggaa aagtcgtcaa gctgagcgag cggggctttc
     1321 gccgagagcg cacgggcgcc gaggatttga atgggctggt gaaactgtat ccggacctca
     1381 gctgcttaat cgtctaccca cgatttgagg tcagagacgt gattgaggtc gcggcgagcg
     1441 ggggtctgtt cccgagcgga acaactcggt tcctgatctc ccctcgggcg ctgcgggtaa
     1501 attttccgct ggcgaccctg gccgatcgct ccccgttaga gagcaagcgg caagtgttgg
     1561 acgagtggat tcgggagcgg gtggagcagc gtagggtccg ctactatgcc gagtccacgt
     1621 tcctcttcga cgagtagtca gctacttgag tccatggctt gatccaggtc ggcgatcaag
     1681 tccccgacag cttccaggcc caccgacaaa cgaatcagcg aaggatcgat ggccagcgga
     1741 gttccggccg tcgaggcgtg ggtcattgcc gccggtacct cgatcaagga ttccacgctt
     1801 cccaatgact cggcgaggct gaacaactgg gtggcctcgg caaatcggac ggccgcggcc
     1861 gcgccggccc gcagtacgaa ggacaccatt ccaccgggct tgcgcatttg ccggttggcc
     1921 aattctgcct gcggcggttc ggcaagtccg gggtagtaca cgacagcaac ccctttcatc
     1981 cgcttcaaat gctccgcaac cagctgggcg ctcgaacagt ggcgatccat ccggaccccc
     2041 agggtcttga tcccccgcag gaccagaaag cagtcgaaag ggccggggac cgcgccaata
     2101 ctgttctgga gcacccgcag ccgaccggcg gtctgctcat ctcggcagat aactgctccc
     2161 ccgaccacgt ccgagtgtcc gctgaggtac ttggtggtcg agtgatgcac tagatccgcc
     2221 ccaagctgaa gcggccgctg caagtaaggt gtcgcaaagg tattgtccac tgcgaccaag
     2281 accccagggc gggaccgctt ggcgagccgt gcaatctcgg cgatatcgca gacacggagc
     2341 agtgggttgg tcggggtttc gagccaaatg aggcgggtct ccgccctcgc ctgggagacg
     2401 aactcggccg ggttagcagg cgaggcgtag ctgaaggaga ttcccagctt cgcatagaca
     2461 gtctcgaata gtcgataggt gccgccgtac aggtcatcta ccgccaggac atggtctccc
     2521 ggctgcagca gatgggcgat ggcgtcgatc gctgccatcc ccgaggcgaa cgcaaggccg
     2581 tttgttccgt cttcgagagc agccagacag cgttccaagg cggtgcgggt gggattgcct
     2641 gtccgtgagt actcataccc acggtggcgg cccacgcctt cctggcgata ggtcgaagtc
     2701 tggtaaatcg gcgtcatgat cgccccagtg gttggatcgg gcggctggcc tgcgtggatg
     2761 atgagcgttt cgaattccgg catgggactc tcctaccccc aattctacag cgaggaagtg
     2821 cgggattaag gctcacggac tgcgcatagc cgcaatccac taactgaatc cggatgggaa
     2881 gaaggtccta ctcctcgtaa aagggcctaa a
//
LOCUS       DATDPQ010000286         4248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1472011, whole genome shotgun sequence.
ACCESSION   DATDPQ010000286 DATDPQ010000000
VERSION     DATDPQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472011"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VI520_06395"
     CDS             complement(<1..1097)
                     /locus_tag="VI520_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLF37545.1"
                     /translation="MKDSTNVVVRPELVGQLKDARIRELELFSDLTEEQLVSPSLPNI
                     EPPLWELGHVGWFQERWILRRLDGAEPLLPQADQLYDSFNIANARRWNLRFPSRSETL
                     DYLAAVLDRCIARLEGREPSDEDVYFYHLATFHEDMHGETLTHSRQTLGYVAPRLSRS
                     RAPETPAAPEPGFELHDVEVPGGSYWLGATADLPFVFDNEKWAHPVAVRPYRISATPV
                     TNVDFQRFVDEGGYRQRELWSEEGWQWRLETKAEHPVYWQPTGDRWLWQRFDALTPLE
                     PFKPVIHVNWHEANAYCAWAKRRLPSEAEWELAASAEPAAGGRGVSDHKRRYPWGDEP
                     PSPARANLDSAAMGCVDVRALPAGDSAFGCR"
     gene            complement(1094..2095)
                     /gene="egtD"
                     /locus_tag="VI520_06400"
     CDS             complement(1094..2095)
                     /gene="egtD"
                     /locus_tag="VI520_06400"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HLF37546.1"
                     /translation="MAGPALAPLHLERIGPQLDDLRDEVLRGLAKPAKELPCKLFYDR
                     RGSALFDQICTLDEYYLTRTETAIIKQNAAEIAALIGERCVLIEYGSGSSSKIRIMLD
                     HLREPLSYIPIDLAEDQLLRSATAIAAAYPRLEVLPLCADYTRPLMLPSRAESDARRV
                     AFFPGSTIGNFHLDEVEGFLRQVRDSLGRQGGLLIGVDLKKDPKLLHAAYNDTAGVTA
                     EFNLNILAHINRRFGADFRLDQFTHRARYDQRHGRIEMHLISRADQAVQFEDTEFRLE
                     AGEPILTEVSYKYDLDQFAELAERAGFGVQRVWTDSAELFSVQYLEPRGSGPGTGRK"
     gene            2242..2598
                     /locus_tag="VI520_06405"
     CDS             2242..2598
                     /locus_tag="VI520_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37547.1"
                     /translation="MLPIALIWAGCGRAVDELLTGPETLEAPAGLPIDPDAPYPPAIA
                     AAIGRLAEDLGVAPQSIEVVSYEEVDWPDGCLGLPAPNEACTEAITPGWRVRLSLAQE
                     IYTFRSDAVGSELREE"
     gene            2600..3712
                     /locus_tag="VI520_06410"
     CDS             2600..3712
                     /locus_tag="VI520_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37548.1"
                     /translation="MGQVISRIRKPVRRSGVERYLLLTLLSFAASVAITRLFLEITGY
                     PQLGNSELHIAHVLWGGLLLFVAALLPLIYANRWVYDAGALLAGIGVGLFIDEVGKFI
                     TQSNDYFFPAAAPIVYTFFLLTVLLYYRVRRPVSNDPREQLYRALEAMEEVLDHDLDR
                     RERAELEDSLDRIAAEGQFGNLAEALRSFLKSEDLWLAPRRLGRLERWVRAARVLEER
                     WITPARHRAVLVGGLLALGGFTLARTLRLMVGSGSPARAAQKLSELVLGGRIGSDRQL
                     GLFQWEVASETGVAALLVLGAALLVLRRDRLAVSTAYAGLLLSLTVVNLLTFYFEQFS
                     TIATASVQFFLLAGLLRYRSRFLASQRDASRQRWSP"
     gene            complement(3687..4205)
                     /locus_tag="VI520_06415"
     CDS             complement(3687..4205)
                     /locus_tag="VI520_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HLF37549.1"
                     /translation="MSELELFRKEKDEFFREGHQSPLLPEQQAGFAGLSYFPENPDLR
                     LAVEVKHLPDDEIQIQTNTGAVQTYRRFGRFKFEVEGQPAELTILENENGYFLPFADS
                     LAGQETYGAGRYLEPERRADGRFEVDFNLAYNPYCAYNERWSCPITPPENRLKVPIRA
                     GERNFKETNAAG"
BASE COUNT          718 a         1299 c         1393 g          838 t
ORIGIN      
        1 tgtcggcaac cgaaggcact gtccccggcg gggagcgctc gcacatcgac gcagcccata
       61 gccgccgagt cgaggttggc gcgcgctggg ctgggtggtt catctcccca cggatagcgg
      121 cgcttgtggt ctgaaactcc ccgtccaccc gccgcgggtt ctgcacttgc agccagttcc
      181 cattccgcct cgctagggag ccggcgtttc gcccaggcgc agtaggcatt ggcctcgtgc
      241 cagttgacgt ggatcaccgg cttgaatggt tctaatggcg tcagcgcgtc gaaccgctgc
      301 cacagccaac ggtcgccggt cggctgccaa tagaccggat gttccgcctt cgtctccaag
      361 cgccactgcc agccctcctc actccacaac tctcgctgcc gatacccacc ttcatcgacg
      421 aagcgctgga agtcgacgtt ggtgaccggg gtggcggaga tgcgataggg acggacagcc
      481 acgggatggg cccacttctc gttgtcaaag acgaagggga ggtcggccgt ggcgcccagc
      541 cagtacgaac cccctggaac ctccacgtcg tgcagctcaa aaccgggttc gggcgcggct
      601 ggggtttccg gagctcttga ccttgagagc ctcggtgcga catagccgag agtctggcga
      661 ctgtgggtga gcgtctcccc gtgcatgtct tcatggaagg ttgccaggtg gtagaagtag
      721 acatcctcgt cgcttggctc gcggccctcc aggcgcgcaa tgcagcgatc gagcacggcg
      781 gcaaggtaat ccagggtctc gcttcgggag gggaagcgaa gattccagcg acgggcgttg
      841 gcgatgttga atgaatcgta gagctggtcg gcttgcggaa gcagaggctc ggcgccatcc
      901 aaccggcgca ggatccagcg ctcctgaaac caaccgacgt gtcccagctc ccagagcggg
      961 ggctcgatgt tcggcagcga cgggctgacc aattgctcct cggtcagatc ggagaagagt
     1021 tcgagctcgc ggatccgggc atccttcagc tgccctacga gctcagggcg cacaacgacg
     1081 tttgtgctat ctttcacttt cggccggtcc cgggcccgga cccgcgaggc tccaggtact
     1141 gcacgctgaa cagctcggct gaatcagtcc acacgcgctg cactccaaat ccggcccgct
     1201 cagcgagttc tgcgaactga tccagatcgt atttgtaaga aacctcggtg aggatgggct
     1261 cgcccgcctc gaggcggaac tcggtatcct caaactggac agcctggtct gcccggctga
     1321 tcaggtgcat ctcgatcctt ccatgccgtt ggtcgtaccg ggcgcggtgg gtgaactggt
     1381 cgaggcggaa atccgctccg aagcgacggt tgatatgggc gaggatattc aggttgaact
     1441 ccgccgtgac cccggctgtg tcgttgtagg ccgcatgaag cagctttgga tcttttttga
     1501 ggtcgacgcc gatcagcagg ccgccctggc ggccgagcga gtccctgacc tgccgcagaa
     1561 acccctcgac ttcatccaga tgaaagttcc cgatggtcga gccggggaag aaggcaaccc
     1621 gccgcgcgtc gctttcagcc cgcgaaggga gcatcagtgg ccgggtgtag tcggcacaga
     1681 gtgggagcac ctccagccgc ggatacgcag cggcaatggc tgtcgccgag cggagcagct
     1741 gatcctcagc gaggtcgatc gggatgtagg agagcggctc gcgcagatga tccagcatta
     1801 tgcgaatctt gctgctgctc ccgctcccat actcgatgag cacgcaccgc tccccgatca
     1861 gcgctgcgat ctcggctgcg ttttgcttga tgatggcggt ctcggttcgg gtcaggtaat
     1921 actcatcgag ggtgcagatc tggtcgaaca gcgccgaccc gcgccggtca taaaagagtt
     1981 tgcagggaag ctccttggct ggctttgcca gcccgcgcaa tacctcgtcg cgcaggtcgt
     2041 ccagctgcgg accgatcctt tccaagtgca ggggggcgag cgcgggtccg gccaagccgc
     2101 gcaattctat cagggccggt tggtgagggg gtaaagaggc tcgtttgctc ggtgaattac
     2161 acttcagatt ggcgaatgac tgctcggttc ccgcgaagag aggggaagcc gcctgcctcc
     2221 ccgactgctc ttcttctggc gatgctcccc atcgcgctga tctgggccgg ctgcgggagg
     2281 gcggtggatg agctgcttac cgggccggag acccttgagg cgcccgccgg gctgcccatc
     2341 gacccagacg cgccctatcc gccggcaatt gcggcggcga tcgggaggct ggcagaggat
     2401 ctgggagtcg caccccaatc gatcgaggtg gtgagctacg aggaggtcga ttggcccgac
     2461 ggatgtcttg gcctaccggc cccgaacgag gcctgcacgg aggccatcac cccgggctgg
     2521 agagtcaggt taagcctcgc gcaggaaatc tacaccttcc gatccgacgc ggtcgggtcc
     2581 gagctcaggg aggaatagga tgggccaggt gatcagccgc atccggaaac cggtgaggcg
     2641 cagtggggtc gagcgctacc tgctgctcac cctgctcagc ttcgcggcct cggtggcgat
     2701 cacccgcttg tttctggaaa tcaccggtta tccccagctc gggaatagcg agttgcacat
     2761 cgcccacgtg ctgtggggag gcttgctgct attcgtggcc gccctgctgc cattgattta
     2821 cgccaaccgc tgggtgtatg acgcgggcgc gctcctagcg gggatcggag tcggactgtt
     2881 catcgatgag gtcgggaagt tcattaccca gagcaacgat tatttcttcc cggccgcggc
     2941 cccgatcgtc tatacgtttt tcctgctcac agtgttgctc tactaccggg tccgccgccc
     3001 ggtttccaac gacccgcgcg agcaactcta ccgtgccctg gaggcgatgg aagaggtgtt
     3061 ggatcacgat ctggatcgcc gcgagcgggc cgagctggag gactccctgg atcggatcgc
     3121 ggccgagggt caatttggca acctggcgga ggcgcttcgc agctttctga aatcagagga
     3181 cctgtggctg gcgcctcgcc ggctgggccg gctcgaacgt tgggtgcggg cggcgcgggt
     3241 gttggaggag cgctggatca cgccggctcg ccatcgggcc gtcctggtgg gcgggctcct
     3301 ggccctgggt gggttcaccc ttgcgagaac ccttcgattg atggtcggaa gtgggtcgcc
     3361 ggcccgcgcc gcacagaaac tttcggagct cgtcctgggg gggaggatcg ggtccgatcg
     3421 ccagctcggt ttgtttcagt gggaagtggc gtcagagacg ggtgtggcgg ccttgctggt
     3481 tctcggagcc gcattgctgg ttctgcggcg agaccggctt gcggtgagta cggcctatgc
     3541 gggcctgctg ctgtcgctga cggtggtcaa cctgctgacc ttctacttcg agcagttctc
     3601 gacgatcgca accgcctcgg tgcagttttt cctgctggct ggtctgttgc gctatcgcag
     3661 ccggttcctt gcctcgcaaa gggacgctag ccggcagcgt tggtctcctt gaagttccgc
     3721 tccccggccc ggatcggcac cttgagccgg ttttcgggcg gggtgatcgg gcacgaccac
     3781 cgctcgttat aggcgcaata cgggttatag gccaggttga agtccacctc gaagcggccg
     3841 tcggcccgtc gttccggttc gagatagcgg ccggcgccgt acgtttcctg gccagccagc
     3901 gaatcggcga acggcaggaa gtagccgttc tcgttctcta agatcgtgag ctcggccggt
     3961 tgcccctcca cctcaaactt gaagcggcca aagcgccgat aggtctggac cgcgccggtg
     4021 ttggtctgaa tctggatctc gtcgtccgga agatgcttga cctccaccgc aagccgcaag
     4081 tccgggttct ccgggaagta gctgagcccg gcaaagcccg cctgctgctc cggaagaagc
     4141 gggctttgat ggccctcgcg gaaaaactcg tctttctctt tacggaatag ttccaattca
     4201 ctcatcgaag ctccatttgg tctaaccaac gaccgcttat atcacacc
//
LOCUS       DATDPQ010000287         2774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3837893, whole genome shotgun sequence.
ACCESSION   DATDPQ010000287 DATDPQ010000000
VERSION     DATDPQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837893"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VI520_06420"
     CDS             complement(<1..507)
                     /locus_tag="VI520_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HLF37550.1"
                     /translation="MSSKQGRRQLLRVIGTLASLGLLAYLVSRQGWQTIWDSIRQISP
                     GRFLLAFALMFLSRLAVTARWTSLLWLSGLRLPAWEILRVTFAGMFASHFLPTSVGGD
                     IVRLAWTVRVQGDRGKYASSVAVDRLVGLAGMAMLLPLGIAQVLGAGVLGSAARIAPA
                     AIGAAASTT"
     gene            complement(504..1472)
                     /locus_tag="VI520_06425"
     CDS             complement(504..1472)
                     /locus_tag="VI520_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLF37551.1"
                     /translation="MADRPASVSVVVPVYNEVDNLRILHGSLTAALSRRVYSLVFVDD
                     GSSDGSLSVLSELAVEDPEHVRLVELRRNFGQTAAIAAGIDHSDGEIIVLIDADMQND
                     PADIPNMLEKIEQGYDVVSGWRVDRQDRLLTRKIPSRAANWLISRVTGVKLHDYGCTL
                     KAYRREVLQGFRLYGEMHRFIPAYAGSVGARIIEMPVRHHPRLHGKTKYGLERTTKVL
                     LDLFTVKFLISYAVKPIYLFGGTGIALILPSLGVLLFLLVRRLTIGTAVLSSPLFQTS
                     TMLIILGFQSILMGFIAELLVRTYHESQAKPTYSIRRTLNIPSPSE"
     gene            complement(1465..2553)
                     /gene="gmd"
                     /locus_tag="VI520_06430"
     CDS             complement(1465..2553)
                     /gene="gmd"
                     /locus_tag="VI520_06430"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HLF37552.1"
                     /translation="MTKAVITGITGQDGSYVAEFLLSKGYEVHGLIRKSSTFNTDRID
                     HLYQDPHDPGAKLFLHYGDLANGEQLTNLIYNVRPDEIYHMGAQSHVKVSFEMPEYTG
                     QVTGLGTTRILEAVRRSGIQTRFYQASSSEMYGDAPPPQSENSLFRPRSPYAIAKVYG
                     YWMAANYREAYGLFVVNGILFNHESPRRGATFLSRKVTRAVARIKAGLQDKLYLGNLE
                     ARRDYGYTPEYVEVMWKMLQQDKADDYTVGTGVAPTIREFVAEAFAYADLDWQQYVEI
                     DPRYFRPTEVDVLLADSGKARRLLDWNPQITYKPLAKIMVDADLEAVGLEPIGEGLKI
                     LEQNFDAWHRWDSGVTAVLKSAGNGFDG"
     gene            complement(2593..>2774)
                     /locus_tag="VI520_06435"
     CDS             complement(2593..>2774)
                     /locus_tag="VI520_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37553.1"
                     /translation="GANLILGHWFIGRYYHTPRLSLEATLAAVAAFGVVYLGVAVLKD
                     GGSGSRSLTVSTPEV"
BASE COUNT          528 a          886 c          855 g          505 t
ORIGIN      
        1 agtagtggaa gccgcggcgc cgatcgccgc cggcgcaatc cgagcagccg aacccagcac
       61 acctgcgccc agcacctgtg cgattcccag cggcagcagc atggccatgc cggcgagtcc
      121 aaccaatcgg tcgacggcca ccgagctcgc atacttcccc cgatctccct gtacccgcac
      181 ggtccaggcc aggcgcacga tatccccccc cacgctcgtg ggaagaaagt gcgaggcgaa
      241 catgccggcg aaggtgaccc gcaggatctc ccaggccggc agccggagtc ctgacagcca
      301 gagcagcgag gtccagcggg ccgtcacggc cagccgcgag aggaacatga gggcgaacgc
      361 cagcaagaag cgcccgggcg aaatctggcg gatcgagtcc cagatcgtct gccagccctg
      421 ccggctcacc aggtaagcca gcagtccgag cgaggccagc gtgccgatga ctcgcagcag
      481 ctggcggcgg ccctgcttgg agctcattcg gatggagatg ggatgttgag ggtgcgccga
      541 atcgaatagg tcggcttggc ttgcgattcg tgataggttc ggacgagcag ctcggcgatg
      601 aaacccatca ggatcgattg gaatccgagg atgattagca tcgtgctagt ctggaacagc
      661 ggcgaactca acaccgcggt tccaatcgtc agccggcgca caagcagaaa gagcaatact
      721 ccgaggcttg gcaaaatcag cgcgatcccc gttccgccga acagataaat tggtttcacc
      781 gcgtaactga tcaggaattt caccgtgaaa aggtcgagca ggaccttggt ggtccgttcc
      841 aacccatact tggtcttccc gtgcagccta gggtggtggc gcaccggcat ttcgatgatc
      901 cgggctccca ccgagccggc gtaggccggg atgaagcgat gcatctcgcc gtagagccgg
      961 aacccctgca gcacttcccg tcggtaggcc ttgagagtac agccgtaatc atgcagcttc
     1021 accccggtca ctctcgaaat cagccaattg gcggcgcggg agggaatctt gcgggtaagc
     1081 agccggtcct ggcgatcgac ccgccagccc gaaaccacgt catatccttg ttcgattttc
     1141 tcgagcatgt tcggaatgtc ggccgggtcg ttctgcatat cggcgtcgat caacacaatg
     1201 atctcgccgt ccgaatggtc gattccggcc gcgatcgcgg cggtctgccc gaagttgcgc
     1261 cgcagctcca ccagccgaac gtgctccggg tcctctaccg ccaactcgct cagcaccgag
     1321 aggctgccat ccgacgagcc atcatctaca aagacgagcg agtacacccg gcggctgagc
     1381 gcagcggtca acgacccgtg caagatgcgc aggttgtcga cctcgttgta aaccgggact
     1441 acgaccgaca cgctggccgg tcgatcagcc atcgaatccg ttccccgcgc tcttcaacac
     1501 cgcggtgacg cccgaatccc agcgatgcca ggcatcgaag ttctgctcca ggatcttcag
     1561 accttccccg atcggctcga ggccgacggc ctccaggtca gcgtccacca tgatcttggc
     1621 cagcggcttg taggtaatct gcgggttcca atccagcagc cgcctggcct tgcctgagtc
     1681 ggccagcagg acgtccacct ccgtcggccg gaagtagcgc gggtcgatct ccacatactg
     1741 ctgccagtcc aggtcggcat aggcgaaggc ttccgccacg aactcgcgaa tggtcggggc
     1801 gaccccggtg ccgacggtgt agtcgtcagc cttgtcctgc tggagcattt tccacatcac
     1861 ctcgacgtat tccggggtgt agccgtagtc ccggcgtgct tccaggttcc ctaagtagag
     1921 tttgtcctgc aatccggcct tgatccgggc cacggcccgg gtcaccttgc gcgaaaggaa
     1981 cgtggcgcct cgccgcgggc tctcgtggtt gaagaggatt ccgttgacca cgaacagacc
     2041 gtaggcctcc cgatagttgg cggccatcca gtagccgtag accttggcga tcgcataggg
     2101 gctgcgcggc cgaaataggg agttctcgct ctggggggga ggggcgtcgc cgtacatttc
     2161 gctcgaggag gcctggtaga agcgggtctg gatcccgctg cggcggacgg cctccaggat
     2221 gcgggtcgtc cccaatccgg tcacctgccc ggtgtactct ggcatctcga agctgacctt
     2281 gacgtgcgac tgcgccccca tgtggtagat ctcgtcgggc cggacgttgt agatgaggtt
     2341 ggtgagctgc tccccgttgg ccaggtcccc atagtgcagg aacaacttgg cccccgggtc
     2401 gtgggggtcc tggtagaggt ggtcgatccg atcggtgttg aatgtgctgg atttacggat
     2461 caggccgtgg acctcgtagc ctttcgaaag caggaattcg gccacgtacg agccatcctg
     2521 gccggtaatc cctgtgatga ctgcctttgt catccagtcc tccgctcccc gaagtaaaat
     2581 tcaggggctg aattatactt cgggggtact gaccgtcaag ctccggctgc ccgatccgcc
     2641 gtctttcaat accgccaccc ccaggtacac gaccccaaac gcggccacgg cggccagggt
     2701 ggcttccagg ctcagccgcg gcgtgtgata gtagcggcct atgaaccagt gcccgaggat
     2761 caggttggcc ccta
//
LOCUS       DATDPQ010000288         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1988801, whole genome shotgun sequence.
ACCESSION   DATDPQ010000288 DATDPQ010000000
VERSION     DATDPQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988801"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VI520_06440"
     CDS             complement(<1..723)
                     /locus_tag="VI520_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HLF37554.1"
                     /translation="MVDEPLILAITGDYFLIPRLEDTARALGFRLEVVQSAADLGAEG
                     PPIPRAIPLTEPLEGPDAAFVRSLVERRPALLLVDTSHDAVPWEHWIQTLKTGSATRR
                     IPVVAFGPHVEAETLQRAIRAGAEEAVSLGRLQASLAEIIQRRARRIDSAALKEACGQ
                     PLSELGAHGLELLNSGEYFEAHEVLEKAWMEAPEWHGYLYRSLLQVSVAYLQVQRANY
                     AGAVKMLLRLKQWLEPLPDRCSG"
     gene            complement(838..1623)
                     /locus_tag="VI520_06445"
     CDS             complement(838..1623)
                     /locus_tag="VI520_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37555.1"
                     /translation="MLARLVGAELAEAILGDLQELRPTIEARWGKVVSWVWLSLEIAR
                     GAAVLTAQRLRAPRRREKEKRSMDNWNVKARRVAVVIGIVASLPAAVLVVGGLLQSDW
                     GTPALQSTLERTLFNRDLVGFRILIHPATVFSGLLLAAGSNLLPLLRLKLERRPGTLV
                     GMLSLRIRAAHLVVALFAVGLLGVILGYGFTENFKVVPTHVSVLESGWQYTAAPVRPV
                     TTFDGYFLPGALRSLEGSRMLFFRLATDEGTWQAVPTPSPSGE"
     gene            complement(1698..2054)
                     /locus_tag="VI520_06450"
     CDS             complement(1698..2054)
                     /locus_tag="VI520_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLF37556.1"
                     /translation="MSRPKPPEVLSRIEEILLLAVWELGEEAYGVPIRRRVEELLNRR
                     LSVGSVYVPLDRMALAGLLSAEFGPPTAVRGGRRKRYYRLTAKGIAALRQTRRLSRRI
                     WAGKPALLQGTHSPRK"
     gene            complement(2251..>2679)
                     /gene="dcd"
                     /locus_tag="VI520_06455"
     CDS             complement(2251..>2679)
                     /gene="dcd"
                     /locus_tag="VI520_06455"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLF37557.1"
                     /translation="IRVADEFKIFTNVHSTIVDPKQFDPAPMVDFQGEVCVIPPNSFA
                     LARTIEYFRIPRSVLTVCVGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKI
                     YANEGIAQVLFFEADEECQISYADKKGKYQRQQRIELPKM"
BASE COUNT          503 a          870 c          849 g          457 t
ORIGIN      
        1 cccgctgcag cgatccggca gcggctccaa ccactgtttc agccgcagca gcattttcac
       61 cgccccggcg taattcgccc gctggacctg cagataagcc acggagacct ggagcagcga
      121 ccggtagagg tagccgtgcc attccggagc ttccatccac gccttttcca gaacctcgtg
      181 cgcttcgaag tactctccgg agttcagcag ctcgagccca tgggctccca actcagagag
      241 cggttggcca caggcttcct tcaaggcggc cgaatcgatc cgccgggcgc gccgctggat
      301 gatctcggcc aaactggcct gaagtctgcc gaggctgacc gcctcctcgg cgccggcgcg
      361 gatcgcccgc tgcaatgtct cggcctcaac atgcggaccg aaggcgacca ccggaatgcg
      421 gcgggtcgcc gagccggtct tcagagtctg gatccagtgc tcccagggga cggcatcatg
      481 ggatgtatcc accagcagca aagcggggcg acgctcgacg aggctccgta cgaaggccgc
      541 gtcggggcct tccaggggct cggtgagcgg aatcgccctc gggatgggcg ggccctctgc
      601 ccccaggtcc gccgcgctct ggaccacctc cagtcggaac ccgagggcgc gggccgtatc
      661 ttccaatcga gggatcagga agtaatcacc cgtgatggcc aggatcagcg gctcatcgac
      721 catacgggtt gattctacgc gagttgagag gccaacgacc gaccgaccgc tcagccctta
      781 ttggaggccg ctaactactg tgtgggctta gcttgggtcc gaggcaagga tctgggatta
      841 ttcgccgctg ggggaaggag taggaaccgc ttgccaggtg ccttcatcag tcgctaagcg
      901 gaagaagagc atacgcgagc cctccagcga tcttagcgcc cctgggagga agtagccgtc
      961 gaaggtcgtg acgggccgga cgggggcagc cgtgtactgc catcccgatt ccagcacgct
     1021 cacgtgggtc ggcacgacct tgaagttctc ggtaaagccg tagccgagga taacgcccaa
     1081 caagccgact gcgaatagcg caacgacgag gtgagcagcc cggatccgga gcgaaagcat
     1141 acccaccagc gttccgggcc gccgttccag cttgagcctc agcagcggga gcagattcga
     1201 ccctgccgcc agcaggagcc ctgagaagac tgtcgccgga tggatcaaga tccggaagcc
     1261 cacaaggtcc cgattgaaca gggtccgctc cagcgtcgac tgaagagccg gcgtacccca
     1321 gtcgctctgc aggagcccgc cgaccacgag caccgccgcc ggaagagagg cgacgatccc
     1381 gatgacgacc gccactcgcc tggccttcac attccaattg tccatcgatc gtttctcctt
     1441 ttcgcgtcgg cgtggggctc tcagccgctg agcggtcaga actgctgcgc cgcgcgcaat
     1501 ttccagggag agccacaccc aggacacaac cttgccccag cgggcctcaa ttgtcggccg
     1561 caattcctgc aagtccccga ggatcgcctc ggccagctcg gccccgacca gcctggcaag
     1621 caaccgctca gtgcgcccga gccacttcga ctcggtcgga gacgctcctt gtgttgctgg
     1681 gcccgaccgc cttctgctca tttccgcggg gaatgggtac cctgcagcag agccggctta
     1741 ccggcccaga tcctccggct cagccggcgg gtctggcgca gggcggcgat gcccttggca
     1801 gtcagccggt agtagcgctt gcggcggccg ccgcggaccg cggtcggcgg tccaaattcg
     1861 gcggacagca ggcccgccag cgccatgcga tccaatggga cgtacaccga accgaccgag
     1921 agccggcggt tgagcagctc ttcgacccgg cgccggatcg ggacgccgta cgcctcttcc
     1981 cccagctccc acaccgccag cagcaagatc tcctcgatcc gcgagagcac ttccgggggc
     2041 ttgggccgag acatatcttc tagattatag aatatattag ccggttgtca agcctgggtg
     2101 agatcgggga gggcccggcc tggagcaagg cctgaagaga atccccggag ataaatgggt
     2161 ccggcagcga tctgcactcc gatcagaagt tggcctacat cggagacaac tgtgtctgcc
     2221 agcggcccgc tcgctgatca ggcggtcgac ctacatcttt ggcagctcga tccgttgttg
     2281 ccgctggtac tttcccttct tgtcagcgta ggagatctgg cactcctcat cggcctcgaa
     2341 gaacagcacc tgggcgatgc cctcgttggc gtagatcttg gccgggaggg gggtggtgtt
     2401 ggagatctcg agggtaacgt agccttccca ttcgggctcg aagggcgtca cgttgacgat
     2461 gatcccgcag cgggcgtagg tcgacttccc gacgcacacc gtgagcacgg accgcgggat
     2521 tcggaagtac tcgatggtgc gggccagggc gaatgaattg ggcggaatga cgcacacctc
     2581 gccctggaaa tcgaccatcg gggccggatc gaactgtttc gggtcgacga tcgtcgagtg
     2641 cacgttggtg aagatcttga attcgtccgc cacccggat
//
LOCUS       DATDPQ010000289         4735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_152999, whole genome shotgun sequence.
ACCESSION   DATDPQ010000289 DATDPQ010000000
VERSION     DATDPQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152999"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VI520_06460"
     CDS             <1..295
                     /locus_tag="VI520_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37558.1"
                     /translation="RAGGAVVLVGISLNPLSLDTTPVWFQEVDLVGAKTFGMEALNGR
                     TLHTFDLVIEQLREGALSASGLITHRFPFLQYRRAVATASNKRSGSIKVTLTF"
     gene            367..750
                     /locus_tag="VI520_06465"
     CDS             367..750
                     /locus_tag="VI520_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37559.1"
                     /translation="MREPEKITIIEGPPPTFELDTDVWLPGLVEGPVPSQVAMCRLRS
                     PNAPELVQRCYRAWRDGQPISLEYRSEEGLTDQAPIIAARWTDVDEGQVLLLWVRLIQ
                     DPFQVHIEFDDSPDDGPDDDFEPLD"
     gene            758..1543
                     /locus_tag="VI520_06470"
     CDS             758..1543
                     /locus_tag="VI520_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF37560.1"
                     /translation="MGELQFLQVDSTDGVLTAVFNRPKANAFNEPMIEEWLAVLKDAE
                     RDDSVRCLLLTGAGRFFSAGQDVTAFSETDGRVSFRQHLHRTYNRVVLRMRKLEKPIV
                     GAINGPAVGAGLGIALATDLRIAAESASFIYGFTGIGLSADSGTSLALPLLAGLARAA
                     ELAFTNRTVTAPEALEMGLVNRVVPDAELLDQARELAASLARGPTRAIGLSKRALNHS
                     VLGSLEATLEYEAELQEIAGRSEDHAEGLAAFLEKRQPKFQGK"
     gene            complement(1563..3944)
                     /locus_tag="VI520_06475"
     CDS             complement(1563..3944)
                     /locus_tag="VI520_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HLF37561.1"
                     /translation="MNFSREMAWIRFMGLAKYLATIQYAIQRDLQEGRIRFGRGRPPA
                     ELPGPLRSARSVPSGADFHFERADLEARFLAPEMVRLTWLPGALPAPYAVVEKPRPVL
                     DCSLKPSGADWLLFTEQLTLQVDPRGGVTFLNHEGEILRQELPPARIGPSWSHKAPLA
                     PEAHIFGTGERALGFNLRYKHVRLWNQDPSGYGPGRDPLYLTIPCYLCVDNRGCYLLF
                     YENSHDGSLGFDAEAAASFKAGALRYYFIAGALERIFELYADLTGLPAMPPLWALGYH
                     QSRYSYETEAEVREIVQRFQEEKLPLSAIHLDIDYMDGYRVFSVNSRRFPRLTRLADD
                     LLKAGVRLVPILNPGVKRDPQFPVYKDGKAKRVFLRSATGKLIHAPVWPGWVGYPDFS
                     APAARAWWGEQYPGLLEAGASGFWHDMNEPVAFAAWGDRTLPISTPHSVDGRGGLHAE
                     VHNLYGLLMNRAGFEALRRLAPDRRPWLLSRSGWAGLQRYAWTWTGDTESSWAALRKT
                     IPTVLGLSLSGIPYSGPDVGGYLGSPSPELYIRWFQLAALMPFFRTHSGKDTPRREPW
                     SFGSATLEQVRKALSLRVRLLPYLYSLAWQTSRTGFPLVRPLFWLDVKDPQLWGIDDA
                     FVLGESLLVAPILQEGARGRELQLPAGSWYELSSGARFEGPARIWLSAELDQSPILVR
                     AGSLLPLEEANRVILQIYPPGEATGSGLLYSDAGDGYRDWRLDRFVLTQSAERVELNW
                     ESTGRFAFPYAQIELQPRGFEAARLLVDGQAVELPQGLIAARPFKQASFELAG"
     gene            complement(4012..>4735)
                     /locus_tag="VI520_06480"
     CDS             complement(4012..>4735)
                     /locus_tag="VI520_06480"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLF37562.1"
                     /translation="LPDILKRYDPDPIRYYVAATMPETRDSDFSWSDFVSRNNDELVA
                     TWGNLANRVISFSYKHWDGKVPQPGELRGPDKELLHIVESGFDTVGRQIEAVRLRAGL
                     AEAMRLAREVNGYLDRAPWFGVIESDRGAAATTVYTALRAIDSLKVMLSPFLPFSSQQ
                     LHRALGYADALFGEQRIETYEERTRSHQALIYAAEGAAGRWEPSRLPVGQQLAKPAPL
                     YKKLDDELVEAERARLGAASEE"
BASE COUNT          878 a         1501 c         1518 g          838 t
ORIGIN      
        1 ccgggcgggc ggcgcggtcg tgctggtcgg catctcgctc aacccgctca gcctggatac
       61 caccccggtc tggtttcagg aggtggacct ggtcggcgcc aagacctttg ggatggaagc
      121 cctcaacggc cgaacgctgc atacttttga cctggtgatc gagcagctcc gggagggtgc
      181 gctatccgca agcgggctga tcacccaccg gtttccattt ctgcagtatc gccgggcagt
      241 cgccacagcc agcaataagc gcagcgggtc gatcaaggtg accctcacct tctagcgtac
      301 cggccgcggt tggggacgct gtggtaagat tcgccgcgcc cttggccggg gagcatcgag
      361 gagctagtga gggaacccga aaagatcacg atcatcgaag gcccgccgcc gacgtttgag
      421 ctggacaccg acgtttggtt gcccggcttg gtggaaggcc cggttccctc gcaggtggcg
      481 atgtgccgct tgcgcagccc caacgccccc gaattggtcc aacgctgcta ccgggcctgg
      541 cgggacggtc agcccatcag cctggagtac cgctccgagg aaggcctcac cgatcaggcc
      601 ccgatcatcg ccgcccgctg gacggacgtg gacgagggcc aggttctgct gctgtgggtc
      661 cgcttgattc aggatccgtt tcaggtccac atcgaattcg acgattcccc ggacgacggg
      721 cctgacgacg acttcgagcc cctagactag cccaccgatg ggcgaactcc aatttttgca
      781 ggttgacagc acagacggcg tgctgaccgc cgtatttaac cgaccgaaag ccaatgcctt
      841 caacgagccg atgatcgagg agtggctggc ggtgctcaag gacgccgagc gggacgactc
      901 ggtgcgctgc ctgctgctga ccggcgccgg ccggttcttc agtgccggcc aggatgtgac
      961 cgccttcagc gagacggacg gtcgagtctc atttcgccag cacctacacc gcacctacaa
     1021 ccgggtggta ctaagaatgc gcaagctgga gaagccgatc gtgggtgcca tcaacggtcc
     1081 ggccgtgggg gccggccttg gcattgcgtt ggcgactgat ctgcggatcg cggccgagag
     1141 tgccagtttc atctatggtt tcaccggaat tggcttgagt gccgattccg gaacttcgct
     1201 cgccctgcca ctgctggccg gactggcccg ggcggcggag ctggcattca ccaaccggac
     1261 cgtgacggcg cccgaggcgt tggagatggg attggttaat cgagtcgttc cggacgccga
     1321 attactggac caggcccgtg agctggccgc ctcgttggct cgcggaccga ctcgggccat
     1381 tggtctcagc aaacgggccc ttaatcactc agtgctggga tcgttggaag ccacgttgga
     1441 gtacgaggca gagctgcagg aaattgccgg ccgcagtgaa gaccatgccg agggcctagc
     1501 cgcattcctc gagaagcggc agccaaagtt tcaagggaag tgatcgcgtc ggccggagtc
     1561 aatcaaccgg caagctcgaa actcgcctgc ttgaacggcc tggccgcgat caagccctgg
     1621 ggcagctcca cggcctgacc gtcgacgagc aatcgggcgg cctcgaaccc ccgaggttgc
     1681 agctcaattt gcgcgtaggg gaaggcgaac cttcccgtcg attcccagtt gagctcgacg
     1741 cgttccgcag actgagtcaa gacaaagcga tccaaccgcc aatcgcggta accatcgccc
     1801 gcgtcgctgt agagcaggcc gctgccggtg gcttcgcccg gtgggtagat ctgcaggatc
     1861 actcgattgg cctcttccag cggaagcagg ctgcctgccc ggaccaggat cgggctctgg
     1921 tccagttcgg ctgaaagcca aatccgggcc ggtccctcga aacgagcccc ggatgacaac
     1981 tcgtaccaac ttccggccgg cagctgcagc tcgcgccctc gagccccctc ctgaaggatg
     2041 ggagccacca gtaggctctc tcctagcacg aacgcgtcgt caattcccca gagctgtgga
     2101 tccttgacat ccagccagaa gagcggccgc accagcggga agccggtgcg gctggtctgc
     2161 caggccaggc tgtacaggta gggcaggagg cggacccgca gactcagcgc ctttctgacc
     2221 tgttccaggg tcgcgcttcc aaaggaccag ggctcccggc ggggcgtgtc tttacccgag
     2281 tgagttcgaa agaacggcat cagcgcagcc aactgaaacc agcggatgta caactcgggg
     2341 gaaggggaac cgaggtagcc tccgacgtcg ggacccgagt aggggattcc ggacaggctc
     2401 agtcccagca cggtcgggat cgtttttcgt aaagccgccc aactgctctc ggtgtctccg
     2461 gtccaggtcc aggcatagcg ttgcagaccg gcccagccgg agcgcgacag cagccacggc
     2521 cggcggtcag gcgccagtcg gcgcagcgcc tcaaagccgg cccgattcat caacagcccg
     2581 tataggttgt ggacttccgc gtgcagcccg ccacggccgt cgaccgagtg gggggtcgat
     2641 atgggcagcg ttcggtcgcc ccaggcggcg aaggccaccg gctcgttcat gtcgtgccag
     2701 aaccctgagg cgcccgcctc cagcagccct gggtactgct ccccccacca agcccgggcc
     2761 gccggcgcgg agaaatccgg atagccgacc caacccggcc agaccggtgc gtggatcagc
     2821 tttcccgtcg ccgaacgtaa gaagaccctt ttggctttgc cgtccttgta gaccgggaac
     2881 tggggatccc gcttgacgcc gggattcagg atcgggacga gccgcacccc ggccttgagc
     2941 aggtcatccg ccaaccgagt caatcgtgga aagcggcgag aattgaccga gaagacccgg
     3001 tagccgtcca tatagtcaat atcgaggtga attgcgctga gcgggagttt ttcttcctga
     3061 aagcgctgga cgatctcccg tacttcggcc tcagtctcat agctgtagcg cgactgatgg
     3121 tagccaagcg cccagagcgg aggcatcgcc ggcagcccgg tcagatccgc gtagagctcg
     3181 aagatccgtt cgagagcgcc cgcgatgaaa tagtagcgca gcgcacccgc tttgaagctg
     3241 gctgcagcct ccgcgtcgaa gccgaggctg ccatcgtggc tgttctcgta aaacagtaag
     3301 tagcagccgc ggttgtctac acacaggtag caggggatcg tcaggtagag cggatcacgg
     3361 ccgggcccat agccgctggg atcctgattc cacagccgta cgtgcttgta ccgcaggttg
     3421 aaccccagcg cccgttcgcc ggtcccgaag atatgagctt cgggcgcgag cggcgcctta
     3481 tgggaccagc tgggtccaat ccttgccggg ggaagttcct ggcgaaggat ttcgccctca
     3541 tggttcagaa aggtcacccc gcctctggga tccacttgga gcgtgagctg ttcggtaaat
     3601 agcaaccaat ccgcaccgct cggtttgagg ctgcagtcca gcaccggccg cggcttttcg
     3661 accacggcat atggtgcggg gagtgctcca ggcagccagg tgaggcggac catctcaggc
     3721 gccaggaagc gggcttcgag atctgcccgc tcaaaatgga aatcggcgcc gctcgggacc
     3781 gagcgggccg accgcagcgg tcctggcaat tcggctggag gccgaccgcg cccgaaccgg
     3841 atccgcccct cctggaggtc gcgttggatc gcgtattgga tggtcgccag gtatttggca
     3901 aggcccatga agcggatcca agccatttcg cgcgagaagt tcatttcaag acagtctggc
     3961 cagttgaacg tggctggccg ggatcagggg gagatcatgg gatgaaggct actattcttc
     4021 tgaggcagcg ccgagccggg cccgctcggc ctcgacgagt tcgtcgtcca gcttcttgta
     4081 gagcggcgcc ggctttgcca gctgctggcc aaccggcaat cggcttggct cccagcgccc
     4141 cgccgcgcct tcggccgcgt atatcagagc ctggtggctg cgggtccgct cttcgtaggt
     4201 ctcgatccgc tgctcgccga agagagcatc cgcgtatcca agcgcccggt gcagctgttg
     4261 cgagctgaac ggcaagaatg gcgagagcat caccttcaga gaatcgatgg cgcgcaacgc
     4321 tgtgtacacg gttgttgccg cggcccctcg gtcggactcg atcactccga accagggagc
     4381 ccgatccagg tagccgttga cctcccgggc cagtcgcatc gcttccgcca gcccggctcg
     4441 caaacgaacg gcttcgatct gacggccgac cgtgtcaaag ccggactcca cgatgtgcag
     4501 cagttcttta tccgggcccc gcaactctcc cggctggggg accttcccgt cccagtgttt
     4561 gtagctgaag gagatcaccc ggttcgccag attcccccag gtggcgacca gctcgtcgtt
     4621 gttccggctc acgaaatcgg accaggagaa gtcgctgtcc cgagtctctg gcatggtggc
     4681 tgcgacgtaa taccggattg gatccggatc atagcgcttc aagatatccg gcaac
//
LOCUS       DATDPQ010000290         5513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1989624, whole genome shotgun sequence.
ACCESSION   DATDPQ010000290 DATDPQ010000000
VERSION     DATDPQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989624"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="VI520_06485"
     CDS             complement(<1..1674)
                     /locus_tag="VI520_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF37563.1"
                     /translation="MIERRSVPRPAPPTAVADPSDASPTVVVIADVETNATQLIERVL
                     KPAGIPAWPAKEGAPKADVLLVDVTQIRGDPMSGLRNRREMGDDAPAIVLAAHFPASR
                     LRSFLKMGVRDVLLKPYREEELREAILALGRSSAREGHTAQLSHNVEGLREELRRRTD
                     EVRMLSEIGRVVAGLDNLDQILARLVEAAAYVTDAEEASIYLEDPETNEVVLRASKQA
                     GERHATLQRLRADDMLVGEVFRTGQPILRQPNMEGGPVKVQTGFMVQSVVKVPIRVQK
                     EIVGVLGVYNRMALRSFNQHHVTVLMSLADWAGVALDRATLVQQAGSPTGSTGPISLA
                     PKGLLEGLDQALSTLDGLLNGASTGVGRPVKSDLLKLLDQLRTLRRMPITVMEPELAT
                     RYVDLTRLVHKAAGEHQLAATRRGLGLIVEAEALIPAFPGERNRVFQVLQALVGAAIR
                     RTPNGRVLIQTHLLNVQNGRSEGILLPEEIELEDGGWAAVTVADTGPGLSPDTVRAFR
                     DPSVDPSAGNLGYGLTLGEIRMIVESMGGVLWYDQTPAATKITFALPTGS"
     gene            complement(1667..2284)
                     /gene="lepB"
                     /locus_tag="VI520_06490"
     CDS             complement(1667..2284)
                     /gene="lepB"
                     /locus_tag="VI520_06490"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLF37564.1"
                     /translation="MADFPFNQPTQPVIARPKPVSFLRQILRFIGSVLQTVLFAAVLF
                     FAVNLVTARIRVEGDSMEPSLHDGQFVVVSRLAYDLPLFQSEPQRGDIVVFRFPLNPE
                     RRFIKRVIGLPGDEVLVQDGAVYINGVQLFEPYLATPPAYKSAWRVGQDEVIVLGDNR
                     NNSSDSQNWGALPLEGIIGKAVLVYWPLENLGWIPHYDLAVAADD"
     gene            complement(2372..3718)
                     /gene="der"
                     /locus_tag="VI520_06495"
     CDS             complement(2372..3718)
                     /gene="der"
                     /locus_tag="VI520_06495"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HLF37565.1"
                     /translation="MLKPVVALVGRPNVGKSTLFNRLAGERLAVTDSQPGTTRDRLIA
                     EAEWNGLAFDLVDTGGVDPDGGEGELLGQVRAQAELAVQEADAILFLVDGESGVSPVD
                     HELAKVLRRHQTTENGKPRPPILLVVNKADNRPRREQAVEFFELGMGDPIPISALHGT
                     GCGDLLDRLTATFDQSGAEPAVEAQVKIAIVGRPNVGKSSLLNRLLGEERVIVSPIPG
                     TTRDAIDTALMFQGTPLTLIDTAGIRRRGRVLPGVEKYSVLRAVRAIERADVALLLID
                     APEGVTAQDAHIAGIILEKWKSCLLLINKWDAVPAKARSQPAFAEQARAQLKFLDFVP
                     VRFISALHGTGVADILPLALQVQQERLRRIPTAELIQILESAQQAHAPPSKVGVRLRF
                     FLAAQVQTDPPSFLFHVNDPELVHFSYARHLENRIRERFSFLGTPIRLTFRSQPRD"
     gene            complement(3731..4975)
                     /locus_tag="VI520_06500"
     CDS             complement(3731..4975)
                     /locus_tag="VI520_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HLF37566.1"
                     /translation="MRPLRWLWGNLGSLLLAFTLAVAVWFVAVISEDPTDTRELPSPV
                     AIDYLGLRQGLLIVGDPPPATGRVTLRAPLSVWDLMSLDSVGLRVDLTGLEEGTHNLQ
                     VEPILNLQPARVEAHQPESVTITLERAATTSVDVEVVTLGEPQLGYRTSAASASPART
                     ILLGAVSQVDQLASVRAEVNVSDQREDFEQEVTLVALDGAGNRLLGLQFDPARVLVTV
                     QIEPRERFQLVSVVPNLEGEEALSEAGYRLTDVSVTPEVVTVFSADPEALEALPGFVL
                     TLPLDLSGATGDLERRLSLALPAGISPVENQGVLVRVSIEPVVGTITLSRVVEVRGLA
                     VGLFAQLSPEAVDIILTGPLPTLNSLQLEDVRVIVDLLDLGPGTHQIPPQVIISQTNV
                     ENEPVFPATIEVTISNTPPPSP"
     gene            complement(4980..>5513)
                     /locus_tag="VI520_06505"
     CDS             complement(4980..>5513)
                     /locus_tag="VI520_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HLF37567.1"
                     /translation="ERTVNLIVSAAERLAEIRYGALITIEREDRLDEYVETGVPLDAR
                     LTTELLIQIFYVNTPLHDGAVIIRGDRIAAASCVMPLSAGGTLAAGTRRKLGLRHRAA
                     LGLSEVSDAVSVVVSEETGIISITHNGKMIRKLDRSRLRNILATFYRPRRIAGLPGWL
                     SDWLTRSGRSRVEAPGD"
BASE COUNT         1022 a         1739 c         1760 g          992 t
ORIGIN      
        1 gctgccggtg gggagagcga aggtgatctt cgtggcggcc ggggtctgat cgtaccatag
       61 gactcccccc atgctctcca cgatcatccg gatctcgccc agggtgagac cataacctag
      121 attcccggcg gaggggtcga ccgagggatc ccggaatgcc cggacggtgt cgggggaaag
      181 gcccgggccg gtatcggcaa ccgttaccgc cgcccaaccc ccatcctcca attcgatctc
      241 ttcgggcagc aagattccct cgcttcgtcc gttttggacg ttcagcagat gggtctgaat
      301 cagcactctg ccgttcggag tgcggcgaat ggcggctccc accagcgcct gcaggacttg
      361 gaagacccga tttcgttctc cggggaaggc agggatgagg gcttcggcct cgacgatgag
      421 gcccagtccg cggcgggtcg ccgccagctg gtgctcgccg gccgccttgt gcaccagacg
      481 ggtcaggtcc acatagcggg tggcgagctc gggttccata accgtgatcg gcattcttcg
      541 cagggtccga agctgatcca gcagtttgag caggtcggac ttcaccggtc ggccaactcc
      601 cgtcgaggca ccgttgagca gaccatccaa cgtggatagg gcctgatcga gtccttcgag
      661 cagtcctttg ggcgccaaac taatcggacc ggtgctgccg gtcggtgacc cggcctgttg
      721 cacgagagtg gcccggtcca gggccactcc agcccagtcc gccagggaca tgagcacggt
      781 cacgtggtgt tgattgaagg atcgaagcgc catgcgattg tacactccaa gcacgcccac
      841 gatttccttc tgaacccgga tcggaacctt gaccaccgac tggaccatga atccggtctg
      901 gaccttgacc ggtccgccct ccatgttggg ttgacgcagg atgggctggc cggtgcggaa
      961 tacttcgccg accagcatgt catcggcccg aagccgctgc agcgtggcat gccgttcccc
     1021 ggcctgcttg gaagcgcgaa gcacaacctc attggtttcc ggatcctcca gatagatgct
     1081 ggcctcctcg gcgtccgtga cgtaggcggc cgcctcgacc aggcgggcca gaatttgatc
     1141 caggttgtcc agaccggcca ccacccggcc gatttcactc aacatccgaa cctcgtcagt
     1201 acgccggcga agctcctcgc gaagaccttc cacgttatgg gaaagctgcg cggtatgacc
     1261 ctcgcgggca ctcgacctgc ccagggccaa gatggcctcc cgcagctctt cctccctata
     1321 cggcttgagc aaaacgtccc gcacccccat tttcaggaaa ctgcgcagcc gggaggccgg
     1381 gaagtgcgcg gccagcacga tggcgggagc gtcgtcaccc atctcacgcc ggttgcggag
     1441 gccggacatc ggatcgcccc gaatctgggt gacgtctacc agcagcacgt cggcctttgg
     1501 cgctccttct ttggccggcc aagctggaat gccggccggt ttcaggaccc gctcaatcaa
     1561 ctgggtggca ttggtttcga cgtcggcgat gacgacgacc gtcggtgagg catcggaagg
     1621 gtcggctacc gccgttgggg gagcggggcg gggaacggag cggcgctcaa tcatcggcgg
     1681 ccacggctag atcgtagtgg gggatccatc ccaggttctc cagcggccaa tacaccagaa
     1741 cggccttgcc gatgattcct tccaagggca gcgctcccca gttctgcgaa tcggaggagt
     1801 tgtttcgatt gtctcccagc acgatgacct cgtcctgtcc gacccgccag gcactcttgt
     1861 acgccggcgg ggtggccagg tagggttcga agagctggac tccattgatg tacaccgccc
     1921 catcctgtac taagacttcg tcgcctggca ggccaatcac ccgcttgatg aagcgccgct
     1981 ccggattcag tgggaaacga aagaccacga tgtcgccccg ctgcggttcg ctctgaaaga
     2041 ggggcaagtc gtatgccagc cggctgacca cgacgaattg cccgtcgtgc aggctgggtt
     2101 ccatactgtc cccttccacc cgaatgcggg ctgtcaccaa gttgaccgca aagaacagca
     2161 cagccgcgaa caagacagtt tgcagcacac tgccaatgaa ccgcaggatc tgccgtaaga
     2221 aactgacagg ttttggacgg gcgatgacag gctgggtcgg ttggttaaag ggaaaatcgg
     2281 ccacgaaagg cattataaga cccggtctag cggcgtgaca agcgtactgg agttaggctg
     2341 gccggttgga ccccggttcg gtcgtgggca gctagtctcg gggctggctc cggaatgtaa
     2401 gccgaatcgg ggtgccaagg aaactgaacc gctctcggat gcggttctcg agatgtcggg
     2461 cataactgaa gtggaccagc tcaggatcgt tgacgtggaa aaggaaggat ggggggtcgg
     2521 tctggacctg agcggccagg aagaaccgca atcgcactcc caccttggaa ggcggggcat
     2581 gcgcctgctg agcgctttcc agaatctgga tgagctcggc ggttgggatc cttcgcagcc
     2641 gttcctgctg gacctggagc gccagtggaa gaatgtctgc cactccggtc ccgtggaggg
     2701 cagagatgaa gcgaaccggt acgaaatcta ggaacttgag ctgggcccga gcttgctcag
     2761 caaacgccgg ctgagaacgg gccttcgccg gcaccgcgtc ccatttgttg atcagcagca
     2821 ggcagctctt ccatttctca aggatgatcc cggcaatgtg ggcgtcttgg gctgtgacgc
     2881 cctccggtgc atcgatcagc agcagggcca cgtccgcccg ctcaatggct ctgaccgccc
     2941 gcaggactga gtacttctcg actcccggca gcactcgacc gcggcgccgg atgccggcgg
     3001 tgtcgatcag ggtcagcggc gtcccctgaa acatcaacgc ggtgtcgatc gcgtctcggg
     3061 tggttcccgg gatcggggag acgatcaccc gctcttcgcc tagcaagcgg ttaagcaggc
     3121 tcgattttcc aacattgggg cggccgacga tcgcgatctt gacctgcgct tcgacggccg
     3181 gctcagcccc ggattggtcg aaagtcgcgg tcagccggtc gagcaggtct ccacatccgg
     3241 taccgtgcag ggcggaaatc ggaatcgggt cccccattcc gagctcaaag aactccaccg
     3301 cctgctcgcg ccgagggcga ttgtcggcct tgttgaccac cagcaggatc ggcggccgcg
     3361 gctttccgtt ttcggtcgtt tgatgccgcc gcagcacttt cgccagctca tggtcaaccg
     3421 gagatactcc actttcgcca tcgaccaaaa acaggatcgc gtcagcctcc tgtaccgcca
     3481 actcggcctg ggctctgacc tggccgagca gttccccctc gcccccgtcc gggtcgaccc
     3541 ctccggtgtc aaccaggtcg aaggcgaggc cgttccattc cgcttcggcg atcagccggt
     3601 cgcgggtcgt gccgggttgg ctgtcggtca ccgccagccg ctcgccagcg aggcgattaa
     3661 agagggtgga tttgcctacg ttggggcgcc cgaccagagc gacgactggc ttgagcacgg
     3721 gggatccaga tcagggcgag ggaggcggcg tgttgctgat cgtcacttcg atcgtcgccg
     3781 ggaagaccgg ctcgttctcg acattggttt gcgaaatgat cacctggggc ggaatctgat
     3841 gggtgcccgg tccaaggtcc aacaggtcga cgattacccg cacatcctca agctggagtg
     3901 agttcaacgt aggaaggggg ccggtcagga tgatgtcgac ggcctctggt gagagctgag
     3961 cgaacaagcc gaccgccagg ccgcggactt ccaccactcg gctcaaggta atggtcccca
     4021 ccaccggctc gatgctgacc cggaccagga cgccctggtt ttcaaccggg gagataccgg
     4081 caggcagtgc caacgagagt cggcgctcca gatctccggt ggccccggaa agatcgagcg
     4141 gcagggtgag gacaaagccc ggcagtgctt ccaaggcctc ggggtctgcg gagaagaccg
     4201 tcacgacctc cggagtcacc gagacgtcgg tcaggcggta gccggcttcg gacagggctt
     4261 cttcgccttc caaattggga accaccgaga cgagttggaa ccgctcccgc ggctctattt
     4321 gaacggtcac caggacccgg gctggatcga actgtaggcc cagcaggcga ttccctgccc
     4381 cgtccaaggc aacgagggtc acttcttgct cgaaatcctc ccgctggtcg ctcacattca
     4441 cttcagcccg caccgacgcc agctggtcga cctgtgagac ggctcccagc aagatcgtgc
     4501 gggccgggga agcgctggcc gcactggtgc ggtacccaag ctgcggctcg ccaagagtca
     4561 cgacctcgac atcgacgctg gtcgtggcgg cccgctcgag ggttatggtc accgattcag
     4621 gctggtgggc ctccacccgg gcaggttgga gattgaggat cggttcaacc tggaggttat
     4681 gggtcccctc ttccagtccg gtcaggtcga ccctcagtcc gacgctgtcc aggctcatca
     4741 ggtcccagac ggagagcggc gcccgaaggg tgactcgtcc agtggccggt ggcggatcgc
     4801 cgacgatcag cagcccctgc cgcagcccca ggtagtcaat cgcgaccggg gagggcagct
     4861 cgcgggtgtc ggtcgggtct tcactaatga ccgccacgaa ccagaccgcg acggccaggg
     4921 tgaaggccag taggagcgag ccgaggttac cccagagcca gcgcagcggt cgcacggcct
     4981 caatctcccg gggcttcgac tcgagaacgg ccggatcgag tcagccagtc gctcagccag
     5041 cctggcagtc cagcgatccg gcgtgggcga tagaaggtgg ccagaatgtt ccgcaaccgg
     5101 ctgcggtcga gctttcgaat catcttgcca ttatgggtga tcgagatgat gccggtctct
     5161 tcagagacga ccaccgacac cgcatcgctg acttcgctga gccccagcgc ggctcggtga
     5221 cgcaggccaa gcttgcgccg ggtgccggcg gccagagtgc caccggcgga cagtggcatc
     5281 acgcaggagg cggccgcgat ccgatcgccc cggatgatca ccgctccgtc gtgcaatgga
     5341 gtatttacgt agaaaatctg gatcagcagc tcggtggtga gcctggcatc caagggcact
     5401 ccggtctcga cgtactcgtc gagccggtcc tcgcgctcaa tcgtgatcag cgccccatac
     5461 cgaatctcgg ccaaccgttc ggccgcgctg acaatcagat tcacagttcg ctc
//
LOCUS       DATDPQ010000291         2694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_154069, whole genome shotgun sequence.
ACCESSION   DATDPQ010000291 DATDPQ010000000
VERSION     DATDPQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154069"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="VI520_06510"
     CDS             complement(<1..1370)
                     /locus_tag="VI520_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF37568.1"
                     /translation="MPEKPKGDGAFKLTYATMFDPPEELHTRFEAALKEQKAELGHEY
                     GMLIGGDEVRSSEQFEDRSPINQEWMLGKFQLGGEAEATRALEAARQATHSWSRTPWR
                     ERVATMRRVADRIDERVYELAAATALEVGKNRMEAVADAAEAAAMIRYACDQMEANEG
                     FIVQMGQDPLKGFSAHNVSLLRPYGVWVVISPFNFPAALTTGPAGAALLAGNCVVMKP
                     ASDTPWTVRLLAECFHQAGLPKGAVNYVTGPGETLGEGLIKSPLVDGITFTGSFNVGI
                     HIYRRFAHGKYPRPSILEMGGKNPSLVSRNADLERAVSGIVRSAFGLQGQKCSANSRI
                     YVEQDVYDQFLTKFVEATQAIKVGDPTDRAVFMGPVINRKAYDDFKEYNEELSEGGQF
                     LSGGHVLTEGELGKGFFCQPTIVSEVEVSHRLWKHEMFLPVTMVHAIESLEQGMQLAN
                     DVSYGLT"
     gene            complement(1508..2353)
                     /locus_tag="VI520_06515"
     CDS             complement(1508..2353)
                     /locus_tag="VI520_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLF37569.1"
                     /translation="MSGYAALAAGTLSLGLSAILVRSAGAPGVVSSFYRMLIATLLLA
                     VPFVRRIRTTNPPARRAIWIAILGGSLFAADLATWATGVTLSGATAPTLLANTAPVWV
                     GIGTLVVFRGRLGASFWLGTLLALLGAGLVLGLETGDELAGSALGLIAGVFYGGYFLV
                     TQRGRELLDSLTYFWLASASSAVVLLLLAVGFRMPLTGYPASSYLNFLAMGVITQVFG
                     YLAVSYALGRFPASIVAPTLLGQPVITALLAVPLLGEILAPLQILGGAAVLAGVVIVH
                     RGQSN"
     gene            complement(2401..>2694)
                     /locus_tag="VI520_06520"
     CDS             complement(2401..>2694)
                     /locus_tag="VI520_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLF37570.1"
                     /translation="RPFLGVSFQMVTAELAEQENLGADQGAWVTQVVPGSPADEAGLQ
                     EGDLIQRVDDQPVDEKHPLPGLINSHQPGDQIDLSVLRGRRELRVEVELGTRP"
BASE COUNT          518 a          861 c          828 g          487 t
ORIGIN      
        1 gtcaacccat aactcacatc gttggccagc tgcatgccct gctccaggct ctcaatggcg
       61 tgaaccatcg tgactgggag gaacatctcg tgcttccaca gccgatggct gacttccacc
      121 tcggagacga tcgtgggctg acagaagaac cctttcccca gctcgccttc ggtaagcaca
      181 tggccaccgc tcaagaactg gccgccctcg gagagctctt cgttgtactc tttgaagtcg
      241 tcataagcct tgcggttgat gaccgggccc atgaacacgg cccggtcggt gggatcgcct
      301 accttgatcg cctgagtggc ttccacgaac ttggtcagga attgatcgta gacgtcctgc
      361 tcgacgtaaa tcctcgagtt ggctgaacac ttttgaccct gcaggccgaa agcggagcgc
      421 acgatcccgg agaccgctcg ctccaggtct gcatttcgcg agaccagcga ggggttcttg
      481 ccgcccatct caagaatgct cggccggggg tatttgccat gggcgaatct ccgatagatg
      541 tgaatgccaa cattgaatga accggtgaac gtgattccgt ccaccagggg gctcttgatc
      601 aacccctccc cgagcgtttc tcccgggccg gtcacgtagt tcactgcgcc cttaggcagt
      661 ccggcctgat gaaagcactc ggccagcagc cgaaccgtcc agggggtatc ggacgccggt
      721 ttcatcacga cgcagttccc ggcgagcagt gccgcccctg ccggccctgt cgtcagggca
      781 gccgggaagt tgaacgggct gatcactacc cagactccat acggacgcag caacgaaaca
      841 ttgtgggccg agaaaccctt cagcggatct tgccccatct gaacgatgaa gccctcattg
      901 gcctccatct gatcgcacgc atagcggatc atcgccgccg cctcggccgc gtcggccacg
      961 gcttccatcc ggttcttgcc aacctccagc gcggtggcgg ccgctagctc gtacactcgc
     1021 tcgtcgatcc ggtcggccac cctgcgcatc gttgccaccc gctctctcca gggggtgcgc
     1081 gaccagctgt gagtggcttg gcgggcagcc tccagggccc gagtcgcctc ggcctcaccg
     1141 cccagttgaa acttacccag catccactcc tggttgatgg gggatcgatc ctcgaattgc
     1201 tccgagctgc ggacctcgtc gccaccgatg agcattccgt actcatggcc aagctcggcc
     1261 ttttgctctt tgagggcggc ctcgaagcga gtgtgaagct cttccggcgg atcgaacatg
     1321 gtggcatagg tcagcttgaa ggcaccgtcg cccttaggtt tttctggcat tggatcgacc
     1381 tccatctggg gaatgtcgag gccgcggggg atccggtcta ggactgacaa accatatgga
     1441 tggccgccgc tttctggaag tatacctgcc tccccggccg gctagatcgc gcgggacgtc
     1501 ggggagccta gtttgactgg ccgcgatgaa cgatcacaac cccggcaagg actgccgccc
     1561 ctcccaggat ctgtagcgga gccagaattt ctccaagcaa cgggaccgcg agcaatgcag
     1621 taattaccgg ttggccgagc agggtgggcg cgacgatcga agccggaaaa cgaccgaggg
     1681 cgtaactgac cgcgagatag ccgaatacct gggtgatcac ccccatcgcc aagaaattca
     1741 ggtaggaact ggctgggtaa ccggtaagcg gcatccggaa tccgactgcg agcagcaaga
     1801 gcacaactgc agagctggcc gaggccagcc aaaagtaagt gagggagtcc agcagctccc
     1861 ggccgcgctg cgtgaccagg aagtaaccgc cgtagaatac gccggcaatc agacccaggg
     1921 ccgagccggc gagctcgtca ccggtctcga ggcccagcac cagaccggct cctaacagag
     1981 ccagcagcgt acccaaccag aagctggccc ccagtctccc gcgaaagacc accagggtcc
     2041 ctattccaac ccagactggc gccgtgttgg caagcagagt gggggcggtc gccccactga
     2101 gggtgactcc agtcgcccag gtggcgaggt cggcggcgaa gaggctcccg cccagaattg
     2161 cgatccagat cgctcggcgg gccggcgggt ttgtggtccg aatgcgtcgg acgaacggga
     2221 ccgccagcag cagggtcgcg atcagcatcc ggtagaagct gctaaccact cccggagcgc
     2281 cggccgatcg gactaggatc gcggagagcc ccaggctgag ggtccccgcg gccagcgcgg
     2341 cgtagccaga aatgactctt ggagagtccg cctggctagg aggatcgggt tgggtgctac
     2401 ctacggccgg gttccgagtt caacttcgac cctgagctcc ctccgaccgc gcaacacgga
     2461 taggtcgatc tggtcgccgg gctggtggga gttgatcagg cctggcaggg ggtgtttttc
     2521 gtcgactggc tggtcatcga cccgctggat gaggtcgcct tcctgcaatc cggcctcgtc
     2581 ggcagggctg ccgggaacca cctgggtgac ccaggcacct tggtcggcgc ccaggttttc
     2641 ttgctcggca agttccgccg tcaccatttg gaagctaact ccaaggaacg ggcg
//
LOCUS       DATDPQ010000292         4221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3841303, whole genome shotgun sequence.
ACCESSION   DATDPQ010000292 DATDPQ010000000
VERSION     DATDPQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3841303"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VI520_06525"
     CDS             complement(<1..753)
                     /locus_tag="VI520_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLF37571.1"
                     /translation="MAALYVTSVEPYSGKTAIALGLAKLLQAKGKRVGYMKPLSTQPW
                     RRPDGQLADEDTDFVRRTLGLGADLVPESPVIVTSAALRKRLDTGDGGDLADQVKRAA
                     ESVRQRVDLLLLEGGGSLREGYTMGLSNIRLAESLDAPALVLVRCHREMQIVDDALAA
                     KHRLGDQLLGVVFNHVPEEMEEFLNEAARPSLEARGIRVLGSLPRRPRLSALSLGELR
                     ELLGAEVISKGFDPDALIETFTVGAMTAEAALS"
     gene            complement(756..2879)
                     /locus_tag="VI520_06530"
     CDS             complement(756..2879)
                     /locus_tag="VI520_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HLF37572.1"
                     /translation="MSLSHFFAPRTVAVIGASTSPTKLGHAVLNNLLRGGFLKEDRRV
                     YPINPTAESILGQPCFRSVLAVPGPIDLAVIVIPYPAVPDALRECGEKAIPAAIVISA
                     GFREAGWEGAERERELLEVARQFSIRLIGPNCLGVIDTITPLNATFSAGMPPSGPMAF
                     MSQSGALGAAILDWAMAGRLGLSKFVSLGNKADVSETDLLQEWADDPALRVILAYIEG
                     LPDGQQFIRVARQVSRQKPVVAIKSGITQSGARAVTSHTGSLAGSEQAYQAAFRQAGV
                     IRADTLEELLDFALAFGYLTPLRGDRVAIITNAGGPGILATDAVERYGLRLARFEPER
                     QAKLEQFLPGAASAANPVDLLGDARADRFDFALQQILDDPQVDAILVLVTPQAMTDME
                     QTADVIVRAAAQASIPVLACLMGQASLGRGPDILQQHNVPRFPFPERAARALYAMNQY
                     RLLRSVPIPQFDTLEVDRARVSGAIQAVRAAGRVSIGDFEAREILLAYGFKAPATELA
                     ASAEQAVEFADRLGYPVVLKVASPEILHKTDIGGVRLGLEGPSEVRDAFDLITYRAQR
                     FLPEARLWGCTVQKMVPPGLEMLVGMNRDPQFGPLVTCALGGVFVEILQDAVFRLAPF
                     SRQEAEAMLTELRAAALFSGVRGRRPVDREALVEALLRVGQLVVDFPEIEELDINPFV
                     VFEAGQGGVALDMRLILSSQVEPRR"
     gene            complement(2792..3535)
                     /locus_tag="VI520_06535"
     CDS             complement(2792..3535)
                     /locus_tag="VI520_06535"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLF37573.1"
                     /translation="MYELELRRLGHTLVAGLDEVGRGALAGPVVAAAVILPLDRFDLA
                     ATLEGVRDSKQLSPLARQHWAAEVRKIALSVGIGRSSARELDRVGVLAATRLAMGRAL
                     AELSWDPSFLLIDHLRLIDLDIPQYAITRGDQSVLSIAAASVIAKVARDRLMERLGDR
                     FPAYGFDRHKGYGTAHHRAALDRVGTCREHRRSFAPVKLTLGRPRSAKIRSRRQPQIL
                     NCLSPTSSPLEPLPSSAPRPALPSSATQS"
     gene            complement(3575..>4221)
                     /locus_tag="VI520_06540"
     CDS             complement(3575..>4221)
                     /locus_tag="VI520_06540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF37574.1"
                     /translation="VIENGSLDRTAEAADRFAQRNPATRVLREPRAGKGLAVRSGMLA
                     ARGDYRFICDADLSMPIEEVVRFLPPALTDFDIAIASRETPGAVRFGEPPYRHWIGRG
                     FNTLVRLLAVPGFQDTQCGFKCFSAEAARALFPIQRLDGWTFDVEVLFLALKLGYRVS
                     EVPIPWYHIRGSQVRLLRDSWQMFVDLFRIRSNWRRGVYSWPAGSKPSVPIREE"
BASE COUNT          800 a         1416 c         1324 g          681 t
ORIGIN      
        1 ggacaaagcg gcctcggcag tcatcgcgcc gacggtgaag gtctcgatca gggcgtcagg
       61 atcgaatccc ttggaaatca cttccgcgcc cagcagctcc cgcagctcgc cgagactcag
      121 cgcagaaagc cgcggccgcc ggggcagtga gccgaggacc cgaatcccgc gggcctcgag
      181 cgaaggccgg gcggcttcgt tcagaaactc ttccatctct tcggggacat gattgaacac
      241 gaccccgagc agttgatcgc caagccgatg tttggcggcc agtgcatcgt ccacaatctg
      301 catttcgcga tggcagcgga ccaacaccaa cgccggggca tccaggcttt ctgccaaccg
      361 gatattcgag agccccatcg tgtatccttc gcgcagcgag ccccctccct cgagaagcag
      421 caggtctacc cgctgccgga cgctttccgc agctcgtttg acttgatccg ccaggtcacc
      481 accgtctccc gtgtcgagcc gcttgcgcag cgcggctgag gtcacgatca ccggggattc
      541 gggaaccaga tctgccccca gacccagagt acggcgaaca aagtcggtat cttcgtctgc
      601 cagctggcca tcgggccgcc gccagggctg agtgctgagc ggcttcatat agccgacccg
      661 cttgcccttg gcctgcagca gtttggccag gccgagggcg atcgcagtct tgcccgaata
      721 cggttctacg gaggttacat ataaggcagc catggttatc tccttggctc tacctgagag
      781 gacagaatca gccgcatgtc cagggcgacc ccgccctgcc ccgcctcgaa taccacaaaa
      841 gggttgatgt cgagctcttc gatctcaggg aagtccacga ccagttgacc cacccgcagc
      901 aaggcctcca ccagcgcttc ccgatcgacc ggccgtcgcc cgcgcactcc ggagaacagg
      961 gccgcggccc gcagctcggt caacatcgct tcggcctcct ggcgcgagaa cggcgccaat
     1021 cggaataccg catcctgcag gatctcgacg aacaccccac ccagggcaca ggtcaccagg
     1081 ggcccgaact gaggatcccg gttcattccc accagcatct cgaggccggg cgggaccatc
     1141 ttctggaccg tgcaccccca cagccgggcc tccggcagga agcgctgtgc ccgataggtg
     1201 atgagatcga acgcgtcccg aacctcggac ggcccttcca gccccaaccg aacccctccg
     1261 atgtcagttt tgtgaaggat ctcgggcgag gcgaccttta gcactaccgg gtatcccagc
     1321 cgatcggcaa actccaccgc ctgctcggcg gaggccgcca gttcggtggc gggagctttg
     1381 aacccatagg cgagcagaat ctcgcgggct tcgaagtcgc cgatgctgac ccgccctgcc
     1441 gcccgcacgg cctgaatcgc gccgctcacc cgcgcccgat cgacctcaag cgtgtcgaat
     1501 tgagggatcg gcaccgaccg gagcagccga tactggttca tcgcgtagag ggcgcgggca
     1561 gcccgttcgg ggaacgggaa tcgcggcacg ttgtgttgct ggagaatgtc cgggccgcgg
     1621 cctaggctgg cctgtcccat caggcaagcc agcactggaa tcgaggcctg tgcagcggcc
     1681 cgcacgatca catccgcagt ctgctccata tcggtcattg cctgaggggt gaccagtacc
     1741 agaatggcgt cgacctgcgg gtcatcgaga atctgctgca gggcgaaatc gaagcggtcg
     1801 gctcgggcgt ccccgagcag gtccactgga ttggcggcac tggccgcgcc gggcaggaac
     1861 tgctcgagct tggcctggcg ttcgggttcg aagcgggcta gccgcagccc ataacgttcg
     1921 accgcgtcgg tcgccaggat cccgggtcct ccggcgttgg tgatgatcgc cactcgatcc
     1981 cctcgcagcg gcgtcaggta tccgaaggcc aaggcgaagt ccagcaattc ttccagtgtg
     2041 tccgctcgga tcactccggc ctgccggaag gcggcctgat aggcctgttc cgaaccggcc
     2101 aacgagcccg tgtgggaggt caccgcccga gccccggatt gggtgatgcc ggatttgatc
     2161 gccaccaccg gcttctgcct gctcacctgg cgggcgaccc taatgaattg ttggccatcg
     2221 ggcagaccct cgatgtaggc gaggatcacc cgcaatgccg gatcgtccgc ccactcctgc
     2281 agcaggtcgg tctcgctcac gtcggccttg tttcccagcg acacgaactt gctcaggccc
     2341 agccggccgg ccatcgccca gtccaggatg gcggcgccca atgcgccgga ctgagacatg
     2401 aaggccatcg gccccgaagg aggcatcccg gccgagaagg tggcgttgag tggggtgatc
     2461 gtgtcgataa ccccgaggca gttcggtccg atcagtcgaa tcgagaactg gcgggccacc
     2521 tctagcagct cccgctcgcg ttcggcgcct tcccaaccgg cctcccgaaa tccggcgctg
     2581 atgacgatgg cggccggaat ggccttctcc ccacactcgc gtaacgcatc cggaacggcc
     2641 ggatacggga tgacgatcac ggccaggtcg atcggaccgg gcaccgcgag caccgacctg
     2701 aagcacggct ggccgaggat cgattcggcg gtcggattga tcggatacac ccgccgatct
     2761 tcctttaaga acccgcctcg cagcaggttg tttaggactg cgtggccgag cttggtaggg
     2821 ctggtcgagg cgccgatgac ggcaacggtt cgaggggcga agaagtggga gagagacaat
     2881 tcaggatctg tggttgtcgg cgcgatcgaa tcttagcact gcgcggccgg cccaacgtga
     2941 gcttgaccgg ggcaaaggac cggcggtgct cccggcaagt cccaacccga tctaacgctg
     3001 cccggtggtg ggcagtgcca taacccttgt gccggtcgaa gccgtaggcc gggaagcggt
     3061 cgccgagtct ctccatcagc cgatcccggg ccaccttggc gatcaccgag gccgccgcga
     3121 tcgacagcac cgactggtct ccccgggtga tggcgtactg agggatgtcc agatctatca
     3181 ggcgcagatg gtcaatcagc aggaagcttg ggtcccagct caattcagcc agggcccgcc
     3241 ccatcgccag gcgggtcgcc gcgagcaccc caacccgatc gagctcccgg gcggaactcc
     3301 gcccaatccc gaccgaaagg gcaatcttcc ggacctcggc agcccagtgc tggcgggcga
     3361 gcggggagag ctgctttgag tctcgcacac cctccagggt cgccgccagg tcaaaccgat
     3421 caaggggcag gatcacggca gccgccacga ccggcccagc cagagctcct cgtccaacct
     3481 catcgaggcc cgcgaccagg gtatgaccga gccggcgcaa ctcgagttcg tacaccagat
     3541 ccggccgacg ggttggagct tgcgtggatc gaccctattc ctcgcggatg ggcaccgaag
     3601 gctttgaccc ggcgggccat gaatagaccc cgcgccgcca gttgctgcga atccggaaca
     3661 gatcaacaaa catctgccag gaatcgcgca gcagtcggac ctggctgcca cggatgtggt
     3721 accaggggat cggaacttca cttactcgat agccaagctt caaggccagg aagagcactt
     3781 ccacgtcgaa ggtccatcca tcgagccgct ggatcgggaa gagggcccga gcggcctcgg
     3841 cgctgaagca cttgaatccg cactgggtgt cttgaaagcc gggcacggcc agcagccgca
     3901 ccagggtatt gaagccgcgc ccgatccagt gccgataggg gggttcaccg aaacggaccg
     3961 ccccgggagt ttcccgggag gcgatggcga tatcaaagtc ggtgagggcc ggaggcagga
     4021 accgaacgac ttcctcgatt ggcatcgaaa gatccgcgtc gcaaatgaag cgatagtcgc
     4081 cgcgggctgc caacatcccg ctgcgcacgg ccagcccctt accggcccgc ggctccctca
     4141 ggactcgagt cgcgggattg cgctgggcaa agcgatcagc cgcctcggcc gttcgatcca
     4201 gactgccgtt ctcaatgacc a
//
LOCUS       DATDPQ010000293         4381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2260796, whole genome shotgun sequence.
ACCESSION   DATDPQ010000293 DATDPQ010000000
VERSION     DATDPQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260796"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VI520_06545"
     CDS             complement(<1..971)
                     /locus_tag="VI520_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLF37575.1"
                     /translation="MKKTYAILFLLIAMAFMLGACAATATPTEEVVAPPPAAATAVPA
                     TAAPPPPPAAEECMYKIGFVTDVGRIDDQSFNQSGYEGVRLAASSLGLSEDCYSFIET
                     TDAADYIPNIEAFTSQGFQIIVTSGFGMTAATREAGLAHPDVTFIGTDQLQVDENFAP
                     APIDNVAGLLFHEDVSGFLAGALAGYMTETNVIGGVYGCSFIPPVVRFEMGYTNGAKY
                     VNPDVQVINVYHPGDLSTCFTDPTFGAETASTLLAEGADIIFAAAGSTGNGALIAGCN
                     AGEQVIGVDFDQFITVPEVQTCILSSATKDLVNGVAALIVSATEGNLT"
     gene            complement(1168..2190)
                     /locus_tag="VI520_06550"
     CDS             complement(1168..2190)
                     /locus_tag="VI520_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37576.1"
                     /translation="MSSYSERGRAASLIGRRRWASLLLMAALAACAPRSNPGSIESRP
                     TEFPSPTPGPTATPTPSLLLIGAPEGSAASELAAWAADHGWGVLTSDPEGASAIVGET
                     RVDLRAIVSFGEHFGGRAAQIAVDVAPLVLVEAGEIEPGPRLSTVGEPGGRHDQAGFL
                     AGVMAGLAGQTGWVGLVESTGGPLEPVYRAGFEAGLRYGCPKCQLVSLTASEATIDGF
                     RGEGVEVVFVVPGPAAAEAGAQLAEGGLWVVWVGDLPTPDLRVAGQVVFAVDGLIQQA
                     LDALQAAETGAAWPYAIENGGIRLGEVDPEAISPGRQRLLQEAYEAVAAGQLDIGIDP
                     VSGQEG"
     gene            complement(2251..>4381)
                     /locus_tag="VI520_06555"
     CDS             complement(2251..>4381)
                     /locus_tag="VI520_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HLF37577.1"
                     /translation="KRELVGVVLVALGIVIVVSALSSGRGFLGSTWLKLLAYTVGWGA
                     LVIPIGLIGAGALLVAPGLRRRLPGVDLEGILGAAVLYLVVLATFHAVLGASDFDQGL
                     SAARAGRGGGLVGAALLSLLVSGLGGAGAAVTLLALLLIGVSLTSGLPASELLRGAGR
                     VWQAIQARFPAVRFNRRKARPVTSSPARSIAPIAAAADRTPPTGRQAFTAEAIGPRLG
                     REDWSLPGIEDILEPGSESKADDELDRKRAKLIEETLRLFGAPATIVTVNRGPTITQF
                     GVEPDFIEARGGRKTRVKVSKVAALADDLALALASRSIRIEAPVPGKGYIGIEVPNAE
                     ISVVALRDVMISPNFEQINSKLRLGLGRDVSGQAIAADLASMPHLLIAGTTGSGKSVC
                     VNALISGLLLQNTPHDLRFLMIDPKRVELTGFNGIPHLLAPVVVDLQRVVPALQWISR
                     EMDDRYRRFAREGVRNIADYNARRQAIQQEPLPYIAVVIDELADLMMMAPDETERVLT
                     RLAQLARATGIHLIIATQRPSVDVVTGLIKANFPARIAFAVASSVDSRVIIDQPGAER
                     LLGRGDMLFQAADASAPMRMQGSYVSEAELSRLIGFWKQAASLSTPVEGVRSLPASAP
                     LTQAPLWEEETQPTLDRDELFEEAVVVLREMRRGSVSVLQRRLRIGYTRAARLIDQLE
                     EAGIVGPADRGSGAREVLDYGTSPGAI"
BASE COUNT          774 a         1497 c         1376 g          734 t
ORIGIN      
        1 gtgaggttgc cctcggttgc agagacgatc agggcagcca caccgttcac caggtccttg
       61 gtagcgctgg agaggatgca ggtctgcacc tcgggtaccg tgatgaactg gtcgaagtcc
      121 acgccgatga cctgctcgcc cgcgttgcag cccgcgatca gcgcgccgtt gccggtcgag
      181 ccagcggcag cgaagatgat gtcggcgccc tcagcgagca gggtggaggc agtctccgcc
      241 ccgaacgtag gatcggtgaa acacgtgctc agatcgcccg ggtggtagac gttgatgacc
      301 tgcacgtcgg ggttgacgta cttggcgcca ttggtgtagc ccatctcaaa gcggacgacg
      361 ggcgggatga acgaacagcc atagaccccg ccgatcacgt tggtctcagt catgtagcct
      421 gccagcgcgc ccgccaggaa gccagacacg tcctcatgga agagcaggcc ggcgacgttg
      481 tcgattggag ctggcgcgaa gttctcatcg acctgcagct ggtcggtccc gatgaaggtc
      541 acatcgggat gagccagccc agcttcgcgg gtcgccgcgg tcatgccgaa ccctgacgtt
      601 acgatgatct ggaatccctg gctggtgaag gcctcgatgt tggggatgta gtcggcagca
      661 tcagtcgtct cgatgaagct gtagcagtcc tcgctcaatc cgaggctcga ggccgccagc
      721 ctcacgcctt cgtagccgga ctggttaaag ctctggtcgt cgatccggcc gacgtcggtt
      781 acgaagccga tcttgtacat acactcttcc gcggccggcg gaggcggtgg ggctgccgtt
      841 gccgggaccg cggtcgccgc tgcaggaggc ggtgctacaa cttcctctgt cggggtggcg
      901 gttgccgcac aggccccgag cataaaggcc atcgcgatta acaagaaaag gatggcgtag
      961 gtcttcttca ttagtttctc tcctccaagt tttttcgtct gttggattct cgtagttgcg
     1021 cctttgggcg cgcaagatca tcgctcagtg ctctcgaaac ctcgctcctt cctgcctgta
     1081 gtgggcgcac tgggacagtc ttccggagag ggccggaacg caccaaaata gtaacatgat
     1141 ctccgagggc tgacaatgaa tcctcgccta accctcctgg ccgctgaccg gatcaatccc
     1201 gatatcgagt tgcccggcgg cgactgcctc gtaggcctct tgcagcaggc gctgccggcc
     1261 ggggctaatg gcctccggat ccacttcccc tagccggatc cccccgttct caatcgcata
     1321 gggccaggcc gccccggttt ctgcggcctg cagcgcgtct aaggcctgct ggatcaaacc
     1381 gtccacggcg aagaccacct gtcccgcgac cctgaggtcc ggggtcggca ggtctccgac
     1441 ccataccacc cataggccgc cttcggccaa ttgggctccg gcctcggctg ccgctgggcc
     1501 tgggacgaca aatacgacct cgactccctc ccctcggaag ccatcgatag tcgcctccga
     1561 agccgtcaaa ctgaccagtt ggcacttcgg gcagccgtag cgcaggcccg cctcgaaacc
     1621 ggcccggtat accggctcca gcggacctcc ggtactctcg accagcccca cccatcccgt
     1681 ctggccggcc agcccggcca tcaccccggc caagaacccg gcctgatcgt gccgcccgcc
     1741 gggttcgccc actgtgctca accgcgggcc cggctcgatc tctccggcct ccaccagcac
     1801 cagcggagcg acgtcgactg caatctgggc tgcccgccct ccgaagtgct caccaaaact
     1861 gacgatcgcc cgcagatcga cccgggtctc gcccacgatc gcactggcac cctctggatc
     1921 agaggtcagc acgccccagc cgtggtcggc ggcccacgcg gcaagctcgc tggcggcgga
     1981 tccttcgggc gccccgatca gcagcaggct gggagtgggg gttgcggtag ggccgggggt
     2041 cgggctaggg aactcggtcg gccgcgactc aatcgatcca ggattcgacc tcggagcaca
     2101 ggcggcgagc gcagccatca atagcaggct tgcccagcgg cgccggccga tcagggatgc
     2161 cgctcggcct cgttcggagt acgagctcat gagagagtgg gtgtattaat ggaagagcag
     2221 gggcgccagc ggtcgagccg ccgcctgcag tcagatcgcg ccgggggaag ttccgtagtc
     2281 cagtacctcg cgagcgcccg agccccgatc ggccgggccg acgatcccgg cctcctcgag
     2341 ctgatcgatc aatcgggccg cccgggtgta tccaatgcgc agcctccgct gcagcaccga
     2401 gaccgacccg cgccgcatct cgcgcagcac caccacggcc tcctcgaaga gctcatcccg
     2461 gtctaaggtt ggttgagttt cctcttccca cagtggagcc tgggtcagcg gggcgctcgc
     2521 cgggagactg cggacgccct cgaccggcgt gctcaggctg gcggcctgct tccagaagcc
     2581 gatcagccgg ctgagctcgg cctccgagac ataggaaccc tgcatccgca ttggcgccga
     2641 ggcgtcggcc gcctggaaga gcatgtcgcc tcgtccgagc agccgctcgg cgcccggctg
     2701 gtcaatgatc acccggctgt cgaccgagga ggcgaccgca aacgcgatcc gggccgggaa
     2761 attggccttg atcaatccgg taacgacgtc taccgagggt cgttgggtgg caatgatcaa
     2821 atggatcccg gtcgctcggg cgagctgggc caatcgggtc aggacccgct cggtctcgtc
     2881 cggcgccatc atcatcaggt cggccagctc atcgatcacg accgcaatgt agggcagcgg
     2941 ttcctgctgg atcgcctggc ggcgggcgtt gtagtcggcg atgttgcgga ctccctcccg
     3001 ggcaaagcgg cgatagcggt cgtccatttc ccgcgagatc cactgtaagg ccggcaccac
     3061 ccgctgcaga tcaaccacga ccggcgccag cagatgtggg atcccgttga agccggtcaa
     3121 ctcaactcgc ttcgggtcga tcatcaggaa ccgcaggtcg tgcggcgtgt tctgcagcag
     3181 cagaccggag atcagcgcgt tcacacacac cgatttcccc gaaccagtgg tgccggcaat
     3241 cagcagatgc ggcatgctgg ccagatcggc cgcgatggcc tggcccgaaa catctcggcc
     3301 cagacccagc cgcagcttcg aattgatctg ctcgaaattc ggggaaatca tcacgtctcg
     3361 gagcgccacg accgagatct cggcgttggg gacctcgatt ccaatgtagc ccttacctgg
     3421 aaccggcgct tcgatccgaa tcgagcgtga ggcgagtgcc agggccagat cgtccgccaa
     3481 tgcagctact ttgctcactt tgactcgggt cttccgcccg ccgcgggcct cgatgaaatc
     3541 cggctcgacc ccgaactggg tgatcgtcgg gccgcgattg acagtgacga tcgtcgccgg
     3601 ggcgccaaac aggcgcaggg tctcctcaat cagcttggcc cgcttgcggt ccagctcgtc
     3661 atccgccttg ctttccgagc ccggttccag gatgtcttcg atgcctggca gactccaatc
     3721 ctcgcgcccg agtcggggcc cgatcgcttc tgccgtgaag gcctgccggc cagtcggagg
     3781 ggtgcgatca gcggccgccg caatcggggc gatcgaacgg gctggagagc tcgtcacggg
     3841 tcgagccttt cttcggttga agcgaaccgc cgggaagcga gcctggatcg cctgccaaac
     3901 tcggcccgcc ccgcgcagca gttcgctcgc tggcagcccc gaggtcaggc tgactccgat
     3961 cagcagcagc gcaagcaggg tgaccgccgc gcccgctccc cccaacccgc tgaccagcag
     4021 cgagagcagc gcagccccaa ccagaccacc gcctcgtcct gctcgggcgg cgctcaatcc
     4081 ctggtcgaag tcgctcgctc ccagcacggc atggaaggtg gccaagacca ccagatagag
     4141 caccgccgcg cccaaaatcc cttcgaggtc caccccggga agccggcgcc tgagaccggg
     4201 cgcgacgagc agcgccccgg cgccgatcag cccgatcgga atcaccaatg cgccccatcc
     4261 gaccgtgtag gccagcagct tgagccaggt agatcccagg aagccgcgac cagagctgag
     4321 ggcgctgacc acgatgacga tcccgagtgc gaccaacacg accccgacca gttcgcgttt
     4381 g
//
LOCUS       DATDPQ010000294        12096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1477136, whole genome shotgun sequence.
ACCESSION   DATDPQ010000294 DATDPQ010000000
VERSION     DATDPQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477136"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VI520_06560"
     CDS             <1..807
                     /locus_tag="VI520_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37578.1"
                     /translation="RIRPSGGWISLNLRESWEYRELIYFFVWRDLKVRYRQTILGVSW
                     AVLQPFLTMVILSLFFGRLAKIPSDGLPYPVFSYAALVPWTFFASSLTTASNSIISSS
                     NMLKKIYFPRMTLPIAAVLSGLVDLFLALLVLLGMMLVYGLVPTANVLWVPALLLLTL
                     VTSLGASLWLSAMNVQFRDVRYAVPFLIQAWMFATPVAYPSSLLSEPWKTVYGLNPMA
                     GVVEGSRWALLGTDTAPGLIILVSTLVAVALLLSGAYYFRRMEKSFADIV"
     gene            815..2080
                     /locus_tag="VI520_06565"
     CDS             815..2080
                     /locus_tag="VI520_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37579.1"
                     /translation="MSNLAIRVENLGKMYHIGGPQRQYDRLGDRLADMALAPFRRALR
                     LFRGQASSAAGLDQEIWALKSISFEVQEGEVLGIIGRNGAGKSTLLKLLSRITDPTEG
                     LADINGRVGSLLEVGTGFHPELTGRENIFLNGAILGMKKKEIERKFDEITAFAEIDKF
                     METPVKHYSSGMYVRLAFSVAAHLEPEILLVDEVLAVGDIAFQRKCLGKMGDVAQQGR
                     TVLFVSHNMGLIQTLCQRGILLQDGRIASDGPIAETLTAYQRTLESAESEDLAIRTDR
                     RGKGRVRLVNVDILDGNDDPAPVLVTGGPARFVFHVSGLINGLSCVFTLVNQLGQRIV
                     NFKSDTPGPADATCPEMGAKFICEVSPLLLMPGRYRLNVLLRGGGEWQDYVEAAKIFD
                     VEGGSLDGRRVKANYEISVCIPHRWTLPQ"
     gene            2105..3370
                     /locus_tag="VI520_06570"
     CDS             2105..3370
                     /locus_tag="VI520_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HLF37580.1"
                     /translation="MKHVLLTSITGMRNRGVEALVVSSVQQLWQRSPELPVSILTRTP
                     DYDALRTADLGPVKLLDEKVLHPPASRLKRYLGLVLPRYRAEGSPQYREIAAAIADAS
                     AVIASGGDVFSSVYGSLSRHLMPLEMALEAGVPVIFLAQSIGPFSTPADAKAWLAVAQ
                     RSALISVREATSYRYVTEELGLAEARVALTADPAFLLEAADAATVRSMLAQLGIVREQ
                     PLIAVAASRGISRFTACDSRKHQAQWVSLISRILEELDAQVLLIPHVQDTFMPNDDRL
                     LATEIMRSLGFHPRLHLAGADHTAAEFKGLISMCDLVVAERMHAAIAGLSSAVCTLAI
                     GYSIKAGGIMSRLLGERSAAEVVIPFADFLDPGLAWSRLCAAWRERESISATLREALV
                     EVRAEAALNFDLIAQLIGQPARTAIESRN"
     gene            3402..4817
                     /locus_tag="VI520_06575"
     CDS             3402..4817
                     /locus_tag="VI520_06575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF37581.1"
                     /translation="MARRLGRAGSPRRVPAWGGLAGGRGRPGTEPAILLQRWTRPELE
                     PAAEARGSLCIAYLIQNAGNDLWEDTGPAVVIKNTVRGLQRAGHQVFVAKLDGRSVLR
                     IDDIDRLGQAQAMSLGLSGSRAFQLLESGVRRLQRRLRLSYYALFDSFRFYEVCLRVL
                     SGNDICHEYGGAFGIGAALACWRLRIPRLVTVEADFLLEKAVSGSPLRGFHGWVAGQG
                     ARLSYRLAQGIITVSEAAKAQLAERWNLDPGKIVVMPNGVDVDLFRPDRDPAAVRAEL
                     GLGDDPVICFVGAFQVWHGLDKLLQGFARVLAARPRARLVLVGDGRARPMVERLAVEA
                     GVSRSVVITGLVEQNRVPAFLAAADVAVLPYPKLGRELWFSPLKLYEYMAAGKAIVAS
                     RSGQNADVIRHGETGLLVEPGDVADLAEKIIHLLDRPQERRRLGENARTQAVARHSWG
                     GYIQRLEAVYRRAISAAIDPD"
     gene            4912..5745
                     /locus_tag="VI520_06580"
     CDS             4912..5745
                     /locus_tag="VI520_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37582.1"
                     /translation="MARVRGMGRIRRTLRGFSSTQEEVRRKVWQRWNNPSGRTTPVFL
                     IGCGRSGTSMVVSHLARSWRTELYNEDNPAAFDKWRIRDLQVIQGLVERSFAELVLFK
                     PILHTYQIRSFLTRFPDSKAIFAFRHYDDVINSSIKRFGLSNRINHVRVWIEEDFGEF
                     ADSPPPAATKDFIRANWDPSMNPETGAALYWLFQNRLFFDLGLHEDQAVKLVHYESIV
                     RGPTEKFRVLCQFLGLTYEPKISEGIYHSSVKRDPPPEIDPGIRAACEDLRQALIGRA
                     L"
     gene            5742..6104
                     /locus_tag="VI520_06585"
     CDS             5742..6104
                     /locus_tag="VI520_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37583.1"
                     /translation="MSESLLGLLRSRPLQLIVLASVALRLAGAATLGNQVVSLPGTAD
                     QVSYHALGLRLLDGHGFPFGKDRWPITRAGEPTAHWSRIYTLSLAAVCWLLGKKVLAA
                     RSIQAVVVGVDHHRTGPG"
     gene            6133..7497
                     /locus_tag="VI520_06590"
     CDS             6133..7497
                     /locus_tag="VI520_06590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF37584.1"
                     /translation="MKITLSSRRFLPPFSGGVDVYADRLARAFRRMGHQTALLAFDLQ
                     EAAAGPEVVVGKDEHQGLPVWRFVLTSVGRFESTFPYAYHKEMGESIRAVLQQERPDL
                     LFVLSFYLSTLAAVEAAKDLGIPVAHIATDFVPICRRSTLIRWDGKACQKGESLKSCA
                     ACFVSHRPLGRWAARILDALPEPALLRFSERSGDGRSRGLSRPLNPYWKQVSLMRDRL
                     RLLRPLRELIDKVFAPTRYTADAFLASGFRPDQVRLLPFAVEESSPLARIRRSAADHC
                     RFLFIGRLQPYKGAHILVQAFNRLDDPKGATLDIYGVPDGYPGYVRDLEAEIGRNARI
                     RFRGRLDPGDLHLAFSEADYFVIPSIWHENSPLILLDALQSRTPVIASNVGGLSDLIA
                     EGKNGFLFPMGDTEGLSRLMARLIREPMVWDPAQTGPGLQRIEEYARAILNPWQEPAL
                     PGRR"
     gene            7505..8431
                     /locus_tag="VI520_06595"
     CDS             7505..8431
                     /locus_tag="VI520_06595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF37585.1"
                     /translation="MRALVLGGNGFIGSHLVDQLVLSGWEVVVLDLIERRYEPAPDAV
                     HLLQGDLTQSYLLREALLGVDVVFHLAWTTVHETSNQDPAADALANLIPSIRLLEDCL
                     RSGIRRVVFTSSGGTVYGQVRATPTPETHPQNPMSGYGITKLAVEHYLQMFGHLYGLD
                     YSILRPSVPYGPRQNPLGSQGAAAVFLYRVARNLPVTIWGSGNVSRDFFFVTDLVDAL
                     LRAATAELSLHRVFNIGGAEVVTLNELLRRVELLLGRRAEVQYRPARSFDADRVHLDT
                     SLARMELGWQPRVPLEEGLRRTAAWMASQLPA"
     gene            8457..9842
                     /locus_tag="VI520_06600"
     CDS             8457..9842
                     /locus_tag="VI520_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HLF37586.1"
                     /translation="MPQDPTLMSRPNILLLTIDSLRPDMLGCYGSPAGLTPNLDRLAA
                     SALLFRQAITGGSWTQAAFPVLLTSSYASWYGGCLGPLSPRRPSPIAALSAHGYHTLG
                     FSTNPLLSREQGYARGFDSFSDLIPEERDPRLRRLKRGEWMLRLPTTHRLARALGARS
                     RPARLYVSAEKVVDEVCRALGRAEAPFFIWAHFMDTHWPYHIEETLKEPGEIAQAWRD
                     LVYMHRANRKGRALTAAERSRFRQLYQEALRFTDAQVGRVLSQLRESGLAEDTVILIV
                     SDHGEELMERSHWGHFEINLYDEILRVPLLIYFPDSRGGERIERQVRTLDLMPTILDL
                     CQCPLPPGIEGISLAPLWKGGAEAYSAPESISEMWRTHRHIIAVRTESRKYIWDSSQP
                     ELRQLFDLKRDPSERSNIAGDEPAEVARLQSIVDSHIRLGGRAGGDAEFVQVETDREV
                     LRRLKDLGYLD"
     gene            10098..11267
                     /locus_tag="VI520_06605"
     CDS             10098..11267
                     /locus_tag="VI520_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLF37587.1"
                     /translation="MDAPRGTVMDFSWTPEQDAYKRAVVDFAKDNLNDEVIDRDRRGE
                     LSLDNWRKCAEFGILGLPVSPDYGGAGEDILTTMLVMEGLGYGCTDLGLVFAINAQMW
                     SVQHPIETFGTEEQKERYLPGLCRGDLIGAHGMTEPDAGSDTASLQARAKRVDGGYVL
                     SGVKTFSTNAPICHLAVVFATLDPGLGRGGITAFLVDREAQGFSVSNDISKMGLRTSP
                     MSEIVLRDCFVPDSARLGSEGAGLAVFNSSMEWERSCILAGNIGAMERQLEGCIRYAR
                     ERRQFGQPIGKFQSVSNRIADMKVRLETARLLLYKVAWLKQNRKPAVVEAAMAKLYLS
                     ECFLQSSLDALRIYGGYGYMTEFEVERQVRDAIGGTLYSGTSDIQRVIIARMLGL"
     gene            11264..>12096
                     /locus_tag="VI520_06610"
     CDS             11264..>12096
                     /locus_tag="VI520_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLF37588.1"
                     /translation="MNPDFVAVTTAEELLVVADAPARRLRFWSPWLLLFVAGWLVVFA
                     RAFPDPVLLASRNWPLIGVGFAGALLGNATAVGGGLVFVPVMTLVYGMAPLLSLKLAL
                     ASQVFGMTSGAVAWAQRRMVPVEGLAPAIPGMLLGSLFGTFVVRPDSVLVKSVFGPIS
                     ITIGVAILILLRHHGVRDRIPANAGLALFLAAVIGGMITGWVAIGQGEVIAAVLMLAF
                     GLRTERSIGLGVVMLSLNSILLVLIHQFVWGDIPWEMAMFTGFGCVFGARMGPYLTQW
                     I"
BASE COUNT         1984 a         3866 c         3932 g         2314 t
ORIGIN      
        1 cgcatccgtc catccggcgg gtggatctcc ctgaacctcc gggaatcatg ggagtatagg
       61 gaattgatct atttcttcgt ctggcgggat ctgaaggtcc gctacaggca gactatcctc
      121 ggcgtctcct gggctgtgct ccagcccttc ctcaccatgg tgatcctcag cctgttcttc
      181 ggccgcctgg ccaagatccc ctcggacggc ctgccctatc cggtcttcag ttatgccgcc
      241 ctggttccct ggaccttctt cgccagctcg ctgaccaccg cctccaacag catcatcagc
      301 agctccaaca tgctcaagaa gatctacttc ccgcggatga ccttgccgat cgcggccgtg
      361 ctctccgggc tcgtagatct gttcctggcg ctgctcgtcc tcctgggtat gatgctcgtg
      421 tacggcctgg tcccgacggc caacgtgctt tgggtgccag cattgctgct gctcaccctg
      481 gtgacctcgc tcggggcgag cctgtggctc tcggccatga acgtgcagtt cagggacgtc
      541 cgctatgcgg tcccgttcct gatccaggcc tggatgtttg cgacgcccgt tgcctacccc
      601 agcagcctgc tctcggagcc gtggaagacc gtttatgggc tgaatccgat ggccggggtg
      661 gtggagggat cgagatgggc cctcctgggg acagacacgg ccccggggtt gatcatcctg
      721 gtctcgacgc tggtcgcggt cgcgctcctg ctcagcggtg catactactt tcggcggatg
      781 gagaagtcct tcgccgacat cgtgtagaga gccgatgagc aatctcgcca tccgtgtgga
      841 gaacctgggc aagatgtacc atatcggagg cccgcagcgg caatacgacc ggctaggcga
      901 ccggctggcc gacatggccc tggcgccatt ccgccgcgcc ctgcggctgt tccgcggcca
      961 ggcctcctcg gctgccgggc tcgaccagga gatctgggcg ctcaagtcta tctccttcga
     1021 ggttcaagag ggcgaggtcc tcggaatcat cggccgaaat ggggccggca agagcaccct
     1081 gctcaagctc ctctcgcgga tcaccgaccc taccgagggc cttgccgaca tcaatggccg
     1141 agtcggctcc ttgctcgagg tggggacagg tttccatccc gagctcaccg ggcgggaaaa
     1201 catcttcctg aacggcgcta tcctgggcat gaagaagaag gagatcgaac ggaagtttga
     1261 cgaaatcacc gccttcgccg agatcgacaa gttcatggag actccggtga agcactattc
     1321 cagcggcatg tacgtcaggc tggcgttctc ggtagctgcc cacctcgaac cagagatcct
     1381 cctcgtcgac gaggtgctcg ccgtcgggga catcgccttt cagcggaagt gcctgggaaa
     1441 gatgggtgat gtcgcccagc agggccgcac ggtcctcttc gtcagccaca acatgggttt
     1501 gatccagacg ctgtgccagc gcgggatcct tttgcaggac ggtcgcatcg cctcggacgg
     1561 gccaatcgcc gagacgctca ccgcttatca gcggacgctc gagagcgccg agtccgagga
     1621 tttggccatc cggaccgacc gacgagggaa gggccgggtc cggctcgtca acgtcgacat
     1681 cctcgatggc aacgatgatc ccgcccctgt gctggtcacc ggagggcccg cccgctttgt
     1741 cttccacgtg agcggcctca tcaacgggct gtcctgcgtg ttcaccctgg tgaatcagct
     1801 cggccagcgg atcgtgaact tcaagagtga tacacccggt ccggcggacg ccacctgccc
     1861 cgagatgggt gcgaagttta tctgcgaggt gagcccgctg ctgttgatgc ccggtcgcta
     1921 ccggctcaat gtcctgctgc gcggcggcgg cgaatggcag gactacgtcg aggcggccaa
     1981 gattttcgac gttgaggggg gaagcctcga tggccggcgg gtgaaagcga attatgagat
     2041 cagcgtctgc ataccgcatc gctggacgtt gccccagtag ccgcggatgg cagccggccc
     2101 gacgatgaaa cacgtcctgc tcacctcgat cacgggcatg cgaaaccggg gtgtcgaagc
     2161 ccttgtcgtc tcgtcggtgc agcagctttg gcagcgaagc ccggagcttc ctgttagcat
     2221 cttgacgagg accccggatt atgacgccct ccggactgcc gatttgggcc cggtgaagtt
     2281 gctcgacgag aaggtgctgc atccgcccgc ttcccgcctt aagcgttacc tgggcctggt
     2341 tttgccccgg tacagggcgg agggatcacc gcaatatcga gagatcgccg cggcgattgc
     2401 cgacgcttcg gcggtaattg cctcaggtgg cgatgtgttc agctcggtgt atggctcgct
     2461 cagccggcat ctgatgcccc tggagatggc ccttgaggcg ggagtccccg tcattttctt
     2521 ggcccagtcc atcggcccgt tcagcacacc ggcggacgcc aaagcctggc tggcggtcgc
     2581 gcagcgctcg gccctgatct cggtgcgcga ggcaacctcc tatcgctatg tcaccgagga
     2641 gctcgggctg gcggaggcaa gggtcgccct caccgccgac ccggcattcc tgctcgaagc
     2701 ggccgatgcg gccaccgttc gctccatgct ggctcaattg ggcatcgtgc gggaacagcc
     2761 gctgatcgcc gtcgccgcga gccgcggcat cagccgcttc accgcctgcg attctcgaaa
     2821 gcatcaggcg cagtgggtat cgctcatctc caggatcctg gaggagctcg acgcccaggt
     2881 cctcctcatc ccgcacgttc aggacacctt catgcccaat gacgatcgcc tgctggccac
     2941 cgagatcatg cgctctctgg gctttcatcc ccggcttcat ctcgccgggg ccgaccacac
     3001 cgcggccgag ttcaaggggc tgatttcgat gtgcgacctg gtggtagccg aacggatgca
     3061 tgcggcgatc gccgggttat cgagcgcagt gtgcacgctg gcgatcggct actcgatcaa
     3121 ggctgggggg atcatgagcc ggctgcttgg cgagcgttcc gccgcggaag tcgtaatccc
     3181 tttcgcggat ttcctggatc ctggtctggc ctggagccgc ctctgcgctg cctggcgcga
     3241 gcgggaatcg atttcggcca cgctccgaga ggcgctcgtc gaagtccgtg cggaggcggc
     3301 gttgaacttt gacctgatcg cgcaactcat cgggcagccg gcaaggaccg cgattgagtc
     3361 ccggaactga cgaagagacc cttcctcggg gaggaggccg ggtggctcgg cgcctgggac
     3421 gtgccggaag cccgcgccgc gtcccagcgt ggggtggctt ggcaggcggc cggggccgcc
     3481 ctgggaccga gccggcgatc ctgcttcagc gatggacccg tccggagctg gaaccagcgg
     3541 cggaagcccg cggttccctg tgcatcgcct atcttatcca gaatgcgggc aatgacctct
     3601 gggaggacac ggggccggcg gtcgtcatca agaacaccgt tcgcggcctg cagagggccg
     3661 gtcatcaggt tttcgtggcc aagctcgatg ggcgctcggt cctccggatt gatgatatcg
     3721 accggctggg tcaggcccag gcaatgtcgc tgggcctcag cggcagcagg gcctttcagc
     3781 tcctggagag cggggtccgc aggctgcaaa ggcgattgcg gctctcctat tacgctctat
     3841 ttgactcctt ccggttctac gaagtctgcc tgcgtgtcct ctcaggaaac gatatttgcc
     3901 acgagtatgg cggcgccttc ggcatcggtg ccgcactggc ctgctggcgg ctgaggatac
     3961 cgcggctggt cacggtggag gccgacttcc tgctcgaaaa agcggtcagc ggatccccct
     4021 tgagagggtt tcatggctgg gtggccggac agggggcaag gctttcgtat cgcctggccc
     4081 aggggatcat cacggtgtcg gaagctgcca aggcgcagct cgctgagcgt tggaatctcg
     4141 accccggcaa gatcgtcgtc atgcccaatg gggtcgacgt ggatctgttc cggccggatc
     4201 gcgatcccgc cgcagtccgg gctgagctcg gattgggcga cgatcccgtg atttgcttcg
     4261 ttggcgcctt ccaggtctgg cacgggctgg acaagctgtt gcagggcttc gccagggtcc
     4321 tggcggcccg gccacgggcc aggctcgtgc tcgtcggaga tggccgggcc cgcccaatgg
     4381 tcgagcggct ggccgtcgag gccggggtta gccgttcggt ggtcatcacc ggactcgtcg
     4441 agcagaaccg ggtcccggcg ttcctggccg cggccgacgt ggcggttctg ccctatccca
     4501 agctgggccg ggaactttgg ttttcaccgc tcaagctgta cgagtatatg gccgcgggaa
     4561 aggccatcgt ggcctctcgg tcgggccaga acgccgacgt catccggcat ggtgagactg
     4621 ggctgctggt cgagcccggg gacgtcgccg atctggcaga gaagatcatc cacctgctcg
     4681 accggcctca ggagcggcgg cgcctagggg agaacgcccg gacgcaggca gtggcgcgac
     4741 attcgtgggg cggatacatc cagcgtctcg aggcggtcta tcgccgggcc atttcagcgg
     4801 cgatcgaccc ggactgaccc tggatcggcc ggatctagcc tgtccggacc ggatctggga
     4861 tcggtcttat ccccaacttc agcttcggca acgaaaggtg atcccttgac catggccagg
     4921 gtacgtggaa tgggccggat ccggcgcacg ctgcgcgggt tctcgtcgac gcaggaggaa
     4981 gtccgacgca aggtttggca gcgctggaac aacccgtcgg ggcgcacgac gcctgttttc
     5041 ctcatcggtt gcggccgttc gggaaccagc atggtcgttt cccacctggc ccggtcgtgg
     5101 cggaccgaac tatacaacga ggacaatccg gccgcattcg ataagtggcg catccgggat
     5161 cttcaggtga tccagggact ggtggagcgc agcttcgctg aacttgtgct cttcaagccg
     5221 atcctccaca cctatcaaat ccgctcgttc cttacgcggt tccccgattc gaaggccatt
     5281 tttgccttcc gccactacga tgacgtcatc aactcgtcga tcaagcgctt cggactatcc
     5341 aaccggatca accacgtgcg ggtctggatc gaggaggact ttggcgagtt cgccgacagc
     5401 ccaccgccag ccgccacgaa ggacttcata cgggccaatt gggatccatc gatgaacccg
     5461 gaaaccggcg cggcgctgta ctggctgttc cagaaccgct tgttcttcga cctgggcctg
     5521 cacgaggatc aagcggtcaa gttggttcat tacgagtcca tagtgcgtgg tccgactgag
     5581 aagtttcggg tcctctgcca gttcctggga ctgacctacg agccgaagat ctcggagggg
     5641 atatatcact catcggtaaa gcgggatccg cccccagaaa tcgacccggg gatccgggcg
     5701 gcgtgcgagg atctgcgcca ggcgctgatc ggacgggcct tgtgagcgaa tctctgctag
     5761 ggctcctcag gagccggccc ctgcagctga tcgtcctggc ctcggtggcg ctccgcctgg
     5821 cgggggctgc caccctcggg aaccaggtgg tcagcctgcc gggcaccgcc gaccaggtct
     5881 cataccatgc gctcggcctg aggctcctcg atggccatgg cttccccttc ggtaaggacc
     5941 ggtggccgat cacccgggcc ggcgagccca cggcccactg gagtcgcatc tacacgctct
     6001 cgctggccgc cgtctgctgg ctcctgggga agaaagttct ggctgcccgg agcatccagg
     6061 ccgtcgtcgt cggcgtcgat caccatcgca cagggccggg atgagccctc ccgcctctcc
     6121 gccgagcccc tgatgaagat cacgctctcc tcacgcagat ttctcccccc attcagcgga
     6181 ggtgtggacg tttatgccga tcgcttggcc cgggcattcc ggcgcatggg ccatcaaacc
     6241 gcgctcttgg ccttcgatct gcaggaggcg gccgccgggc cggaggtcgt ggtggggaag
     6301 gacgagcatc aaggcctccc ggtctggcgg tttgtcctca cctcggttgg gcgctttgaa
     6361 agcacctttc cctacgccta ccacaaggaa atgggcgaga gcatccgggc agtcctgcaa
     6421 caagaacggc cggatctgct cttcgtcctc agcttttacc tctcaacgct ggccgccgtg
     6481 gaggcagcca aggacctcgg aatccccgtg gcgcacatcg ccacggattt cgtgccgatc
     6541 tgtcgccgtt cgaccctgat ccgctgggac ggcaaggcct gccagaaggg cgagtcgctg
     6601 aagtcctgtg ctgcctgctt cgtctcccat cggccgttgg gacgatgggc cgcaaggatc
     6661 ctggatgcgc tacccgagcc cgccctactc aggttttccg aaaggtccgg cgacggccgc
     6721 tcgcggggcc tgtcgcggcc cctcaacccc tactggaagc aggtctctct gatgcgggat
     6781 cgcctccggc tcctgcgacc gctgcgagag ttgatcgaca aggtctttgc gcccaccaga
     6841 tatacggcgg acgccttcct ggcctccgga tttcgcccgg atcaggtccg cctgctgccg
     6901 ttcgccgtcg aggagtccag cccgctggcc aggatccgca ggagcgccgc cgaccactgc
     6961 cgtttcctct tcatcgggcg cctccagccg tataaggggg cgcacatcct cgtccaggcc
     7021 ttcaaccgtc tggacgaccc taaaggggcc acgctggata tctacggcgt ccccgacggc
     7081 tatcccggtt atgtgcgcga cctggaggcc gagatcggcc ggaatgcccg gatccgcttt
     7141 cgcgggcgcc tcgatccggg ggatcttcac ctggccttct ctgaggcgga ttacttcgtc
     7201 atcccctcga tctggcacga gaacagcccg ctgatcctcc tcgacgccct gcagtcgcgg
     7261 acgccggtga tcgccagcaa cgtcggcggc ctgtcggatc tgattgcgga ggggaagaac
     7321 ggatttctgt tcccgatggg cgatacggaa ggcctgtcgc ggctgatggc ccgcctgatc
     7381 cgggagccga tggtctggga tccggcgcag accggccccg ggctgcagcg gatcgaggag
     7441 tacgctcgcg cgatcctgaa cccctggcag gagccggcgc tgcccgggcg gcgatagagg
     7501 ccatatgagg gcgcttgtat tgggaggcaa tgggttcatc ggctcgcatc tagtcgatca
     7561 actggtgctc tcgggttggg aggtcgtcgt cctggacctg atcgagcgcc gctacgagcc
     7621 cgccccggac gctgtccact tgctccaggg cgacctgacc cagagttacc tcttgcgaga
     7681 ggcgcttctt ggcgtggacg tggtctttca cctggcctgg acgacggtcc acgagacttc
     7741 caaccaggat ccggccgctg acgctctggc caacctgatc ccatcgatcc ggctgctcga
     7801 agactgcctt cgcagcggga tcaggcgagt ggtgttcacg tcttccggcg gcacggttta
     7861 tggccaggtc agggccacgc ccacacccga aacgcatccc cagaacccca tgagtggcta
     7921 tgggatcacc aagctcgccg tcgagcatta cctgcagatg ttcggtcacc tctatgggct
     7981 cgactactcg attctgcggc catcggtgcc ctatggcccc cgccagaacc cgctgggcag
     8041 tcagggcgcc gcggccgtct tcctctatcg tgtcgcccgc aacctgccgg tgacgatctg
     8101 gggcagcggc aacgtcagcc gcgatttctt cttcgtcacg gatctggtgg acgcgctgtt
     8161 gagagccgcg accgccgagc tgagcctgca ccgggtcttc aacatcggcg gtgcggaagt
     8221 ggtcacgttg aacgagctgc tgcgtcgggt ggagctcctc ctgggccggc gcgccgaggt
     8281 ccagtatcgg ccggccagga gctttgacgc cgatcgagtc cacctggata ccagcctcgc
     8341 ccggatggag ttgggctggc agcccagggt gccgctggag gagggcctgc ggcgcacggc
     8401 tgcatggatg gcctcccagc tccccgcata gccagcgatc gagtggtttt ttcgccatgc
     8461 cccaggaccc gaccctgatg agccgcccga acatcctcct gctgacgatc gattcgctgc
     8521 gccctgacat gctcggctgc tacggatcgc cggcgggact cacgccgaat ctggaccggc
     8581 tcgccgcatc agcgctcctc ttccggcagg cgatcaccgg cggatcgtgg acccaggccg
     8641 ccttccccgt cctcttgacc tcgagttacg cctcttggta tggcggatgc ctggggccgc
     8701 tgtcaccccg gcggccgtcg cccatcgcgg ccttatccgc tcacggatat cacacgctgg
     8761 ggttctccac taacccttta ttgagccggg agcagggata cgcccggggg tttgattcct
     8821 tctcggactt gatcccggag gaaagggatc cccggctacg ccggctgaag cgcggcgagt
     8881 ggatgttgcg cctgcccacc actcaccgcc tggcccgcgc tctgggcgcc cgcagccgcc
     8941 cggcccgcct ttacgtctcg gccgagaaag tggtcgacga ggtctgtcgc gccctgggca
     9001 gggcggaggc accgttcttc atctgggctc atttcatgga cacgcactgg ccttaccata
     9061 tcgaggaaac cctgaaggag cccggtgaga tcgcccaggc ctggagagat ctggtctaca
     9121 tgcaccgggc gaatcgaaag gggagggccc tcaccgctgc cgagcgttcc cgcttccgcc
     9181 agctgtatca ggaagccctg cgattcacgg acgcgcaggt aggacgggta ctgtcgcagc
     9241 tgcgagagtc ggggctggcg gaggacacgg tcatcctgat cgtctccgac cacggcgagg
     9301 agctcatgga gcgctcccac tggggccact tcgagatcaa cctttacgat gagatccttc
     9361 gggtccccct gctgatttac ttccccgatt cgagaggcgg cgagcggatc gagcgacagg
     9421 ttcgcacgct ggacctcatg ccgacgatcc tggatctatg ccagtgcccg ctgccccccg
     9481 ggatcgaggg gatcagccta gcccccctgt ggaagggcgg agcggaggct tattcagccc
     9541 cggaatccat cagcgagatg tggcgcaccc accgccatat catcgccgtc cggaccgaat
     9601 cgcgtaagta catctgggac agcagccagc ctgagctgcg gcagctgttc gatctgaaaa
     9661 gggacccatc cgagcgctcc aacatcgccg gcgacgagcc cgcggaggtc gcccgcctgc
     9721 aatcgatcgt ggacagccac atccgcctgg gcgggcgggc aggtggagac gccgaattcg
     9781 tgcaggtcga aaccgatcgc gaggtcttgc gacgcctcaa ggacctcggc tatcttgatt
     9841 agggatagtc aaccccggac ccgggagaga gctgtttgga aggcggcgga ccggcggcac
     9901 cgggcgcccc gggctcgcct caccatgggc agaacggccg ggcgctccgg ctggccgggg
     9961 catcccggtt ccacggcgcc cctggaaccg tccccggcca ggtggagtcc tcgcgggcaa
    10021 ccctcagctg atcgtcgggt gggaaaaccg gtttctctgc tggacaaccc ggacgcaacg
    10081 acgagcggcc tccggctgtg gacgcgcctc gaggtacggt gatggatttc tcctggaccc
    10141 cggaacaaga tgcgtacaaa cgggccgtcg tggacttcgc caaagacaac ctcaacgacg
    10201 aagtcatcga tcgggatcgg cgcggagagc tgtcgctgga caactggcgc aagtgcgccg
    10261 agttcgggat cttggggctt cccgtgtcgc cggactacgg gggcgccgga gaagacatcc
    10321 tcacgacgat gctggtgatg gaagggttgg gctacggatg taccgatctt ggacttgtgt
    10381 tcgccatcaa tgcgcagatg tggagcgtgc agcacccgat tgagaccttt gggacagagg
    10441 agcagaagga acgatacctg cccgggctgt gccgaggaga cttgatcggg gcgcacggga
    10501 tgacggagcc cgacgctgga tcagatacag ccagcctgca ggcgcgcgcg aagagggttg
    10561 acggcgggta tgttctctcc ggcgtaaaga ccttctcgac caacgcccca atctgtcatc
    10621 tggccgttgt atttgcaaca ctcgaccccg ggctaggacg gggcgggatc accgccttcc
    10681 tcgtcgatcg ggaggcgcag gggttctcag tcagcaacga catttccaag atgggactgc
    10741 ggacctcccc gatgagcgag atcgttctgc gggactgctt tgtccccgat tcagcgagac
    10801 tgggctcaga aggcgccggc ttggcggtgt ttaacagctc gatggagtgg gagcgcagct
    10861 gcatcctggc cggcaacatc ggtgccatgg aacgacagtt ggagggctgc attcgctatg
    10921 cccgcgagcg gcgccagttc ggccagccaa tcggcaagtt ccagtccgtg tccaatcgga
    10981 tcgccgacat gaaggtccgc ttggagactg ctcggctgtt gctgtacaaa gtggcctggc
    11041 tcaaacagaa cagaaaaccc gcggtcgtcg aagccgccat ggccaagctg tatctgagcg
    11101 aatgcttcct tcaatccagc cttgacgccc tccggattta cgggggctac gggtatatga
    11161 ccgaattcga agtcgagcgg caggtccgcg acgcgatcgg gggaaccctg tactcgggca
    11221 cctccgacat tcagcgggtg atcattgccc ggatgcttgg gctgtgaacc cagatttcgt
    11281 ggccgttaca acggcggaag agctcctcgt ggtagccgac gcccccgccc ggcgcctgcg
    11341 attctggagc ccatggctgc tcctcttcgt ggcgggctgg ctggttgttt tcgcaagggc
    11401 attcccagac ccagttctgc tcgccagccg taactggccg ctgatcgggg tggggtttgc
    11461 gggggcgctc ctgggcaacg ccacggcagt gggagggggc ctggtgttcg tgccggtcat
    11521 gaccttggtc tacggaatgg cgcccttgct gtccctcaaa ctcgcgctag cctctcaagt
    11581 gttcggcatg accagcgggg ccgtcgcctg ggcgcagagg cgcatggtcc cagtcgaagg
    11641 cctggcccca gcaatcccgg ggatgttgct cgggagtctg tttgggacct ttgtcgtccg
    11701 gccggattct gtgcttgtga agtctgtctt cgggcccatt tcgatcacga tcggggtcgc
    11761 catcctgatc ctgcttcgcc accatggggt ccgggaccgc atcccggcga acgcaggctt
    11821 ggccctcttc ctggcggcag tcatcggggg catgatcacc ggatgggtgg ccattgggca
    11881 aggcgaggtg atcgcagcgg ttttgatgct cgcgttcggc ttgcggacgg agcgcagcat
    11941 cggactgggt gtcgtcatgc tgtcgctgaa ctcgatcttg ttggttttga tacaccagtt
    12001 cgtctggggc gacatcccgt gggagatggc gatgttcacc ggttttgggt gtgtcttcgg
    12061 ggcccgcatg ggaccgtacc tgacgcagtg gatcga
//
LOCUS       DATDPQ010000295         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1734696, whole genome shotgun sequence.
ACCESSION   DATDPQ010000295 DATDPQ010000000
VERSION     DATDPQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734696"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VI520_06615"
     CDS             <1..355
                     /locus_tag="VI520_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37589.1"
                     /translation="SREPSPPEMLAAGDPSKADVWSMGILLYRLLVGREPFLGITDRS
                     VTSAILRAIPPSMIKRRQDLPRELDQFVIEGCLAKEPKLRVTIREFNSVIDRYSGDWR
                     VKRAPRKRRRFLGRR"
     gene            409..>2029
                     /locus_tag="VI520_06620"
     CDS             409..>2029
                     /locus_tag="VI520_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLF37590.1"
                     /translation="MSKQQVRSAAASHIGASLKRSLLEDRSRAETIRTAGGLLLSLAV
                     VADGIGGENAGERAAELTVTTIYEYCQNSEESNLPRLLQAALTQANQQVHSESKKSRR
                     KTNMGSTAAIAAIADGRLYVASAGDSRVYLLRGQKAFPLTVDHTWENEVVQSGRLSRQ
                     EAAKHPRKDQIVRSVGYEPELTVDLGLWLRGGEETAAEARAAQGLPLKPGDVVLVCSD
                     GLIKSRHDRAVAHYVEASEFAPMVRGRSARGSVDALIKRALSRQVDDNVSAAILQVPG
                     GVYLRRYLAPAAGAAAALGLMAVVGVWAAPRLSASLGGPAANPTIPALPSGVAFVSEI
                     GGRGETAGPGKAFVPLQAEQLIAAGAGVKLKAIGIASYLRLGLADQSIMYLGPDSQLE
                     LLQIGGQSGTALVLESGVVLVSGQGAAGQVVSISAPSGALARMSGSLMGASWDPIAGQ
                     FEVDCFGGECEILLSGGVGFRLAAGEHLLFTLDGEILGPSPIAHERYAFAGFAGGLVA
                     APTAVKGGSAGAVATPTRTPLGPLFVTPTPPP"
BASE COUNT          348 a          611 c          701 g          369 t
ORIGIN      
        1 gtcgcgcgaa cccagtccgc cggaaatgct ggccgccggt gatccttcca aggcagacgt
       61 gtggagcatg gggatcttgc tctaccgatt gctggtcggg cgggagccgt tccttggaat
      121 cacagatcga agtgtgacct ccgccattct cagggccatt cccccctcga tgatcaagcg
      181 acggcaggac ctgccgcgcg aactggacca gtttgtcatc gaaggctgcc tggctaagga
      241 accgaagttg cgagtcacca tacgcgagtt caactcggtg attgaccgct attcgggcga
      301 ctggcgggtc aagcgggcgc ccagaaagcg gcggcggttc cttgggaggc gctagctgaa
      361 gacgggagtg ggtaggtgaa taacagggtc ccagcccgcg ggagatcatt gtccaaacaa
      421 caagtgcgct cggccgccgc ctcccacatt ggggcttcgc tgaaacgcag tttgctcgag
      481 gatcggtctc gggccgagac aatccgcacc gcgggcgggc tgctgctctc attggctgtg
      541 gtcgcagacg ggatcggtgg agagaacgcc ggtgagcggg cggcagagct gaccgtgacg
      601 accatttatg agtactgtca gaattctgag gagagcaatc taccgcgcct gctgcaggct
      661 gccctgacgc aggccaacca acaggtccat tcggaatcga agaagagccg gcgcaaaacc
      721 aacatgggct cgaccgccgc catcgcggct atcgccgacg ggcggcttta cgttgcgagc
      781 gcgggcgaca gccgggtcta cctgctgcgc ggccaaaagg ccttcccgct caccgtagac
      841 cacacctggg agaatgaggt cgtccaatct ggaagactgt cgcgccagga ggccgccaag
      901 catccgcgca aggaccagat cgtgcgatcg gtcggctatg agcctgagtt gaccgtggat
      961 ttaggcctgt ggctccgggg cggtgaggaa actgcagcag aagctcgggc cgcccagggt
     1021 ttgccgctca agcccggcga tgtggtcctg gtctgctcgg atgggctcat caagtcgcgg
     1081 cacgatcggg cggttgccca ctacgtggaa gccagcgaat tcgcgccgat ggttaggggt
     1141 cgttccgcgc gggggtcggt cgacgcgctg atcaagcgcg ccctgagccg ccaagtggat
     1201 gacaacgtgt cggccgccat tctccaagtg ccgggtgggg tctacctccg acgctacctc
     1261 gccccggcgg ccggcgcggc ggccgcgctg ggtctgatgg cagtggtcgg ggtctgggct
     1321 gcacccaggc tctcggcctc gcttggcggg cctgccgcga atccgacgat accggcgctc
     1381 ccttcgggag tggctttcgt ctctgagatt ggtggccgcg gtgagaccgc cgggcccggt
     1441 aaggcgttcg tgccactgca ggccgaacag ctcatcgcgg ccggtgcggg agtgaaactc
     1501 aaagccattg gcatcgcaag ctaccttcga ttggggctgg ccgatcagag catcatgtac
     1561 ttgggtccgg acagtcagct cgaactgctc cagatcggag ggcagagcgg cacggctctg
     1621 gtgctggagt caggcgtggt tcttgtgtca gggcagggcg cggccgggca ggtcgtttca
     1681 atctcggcgc cctccggagc gctggctcgg atgagcggct cgctgatggg agcaagttgg
     1741 gatcccatcg caggccaatt cgaggtggat tgctttggcg gagaatgtga gatcctgctc
     1801 tcgggtggag tcggattccg gctggcggcc ggggagcacc tgctgttcac cctcgacgga
     1861 gaaatcctgg gtccctctcc gattgcgcat gagcgctatg cgtttgctgg atttgccgga
     1921 ggtctggtgg ccgcgccaac cgcagtcaag ggcggatccg caggagcggt tgctactcca
     1981 acccgcacgc cgctgggtcc gcttttcgtc acgcctaccc caccgcccc
//
LOCUS       DATDPQ010000296         4864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2774554, whole genome shotgun sequence.
ACCESSION   DATDPQ010000296 DATDPQ010000000
VERSION     DATDPQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774554"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /gene="coaBC"
                     /locus_tag="VI520_06625"
     CDS             complement(<1..1097)
                     /gene="coaBC"
                     /locus_tag="VI520_06625"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLF37591.1"
                     /translation="MPDPSSEVPLLKGRRILLGVCGSIAAYKAAELASRLTQAGVEVD
                     TVLTESAQKFVSGLTFQSLTGRRAYTDSDLWSQEGHVLHIGLGQRAQLLVVAPATADT
                     IARLAQGRADNLLSITALAAGCPIVVAPAMDAGMFEHPATQVNLAILRERGVILLGPV
                     EGRMASGLVGLGRMLEPEQLLGHIRRILGRAGPLAGRKVVVTAGGTQEPLDPVRVLAN
                     RSSGKQGFALAQAAIDRGAEVVLIAGPTSLGTPVGVARVDVHTAAQLAEAVSAALPQA
                     AVLLMAAAVADFRPTRAERDKIKRQAGVPKVTLEPTEDILTSLGDRRPKLVVGFAAES
                     DDLLANARSKLEEKDLDLIVANDITASDAGFG"
     gene            complement(1081..1668)
                     /locus_tag="VI520_06630"
     CDS             complement(1081..1668)
                     /locus_tag="VI520_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37592.1"
                     /translation="MNKKRLIDWPYVVGLLLLPIAVVLVLHGVRAVQELTRYDESYFT
                     EDFLNRYETPGSVAIDLEQALRHGDSQLMAGLVGTRGGPAPIEPRPSLIYIFLLSVDG
                     DYFQYLYFNADDYNRVVQYVTEVRGRYVASEPDLYFYMDSGRWRGVAGPIAMSWWILV
                     TVFTAGVLVYRRTAVSRQKMYEQQSGQRQAGAGPK"
     gene            complement(1665..2579)
                     /locus_tag="VI520_06635"
     CDS             complement(1665..2579)
                     /locus_tag="VI520_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLF37593.1"
                     /translation="MSSTGLFHLGVVYLLWGSTFLAIRVAVREGSGFPPFTMGATRVL
                     TAGLILLVIARLRGKRIVPTRAELPVLIGSGLLLWTAGNGLVTWAEQRAASGLAAMMI
                     GTVPIWVGVYEAFLDRRPPSALLLVSLLTALTGIGLLSAPVLSSGVPRDGLSILGLAL
                     AAMTWAAGTVWQSRRKVQLGLQASSAYQHLAGGLGFLIVSRVVAEPVANPVREAWAAW
                     AYLLIFGSIIGYTSYVQTLRLLPISVATTNAYVNPVIALVLGSILLREELTPLTLAGA
                     ALVLAGVAGVFRDRFGPKRRVAVSGGGE"
     gene            complement(2576..3349)
                     /locus_tag="VI520_06640"
     CDS             complement(2576..3349)
                     /locus_tag="VI520_06640"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLF37594.1"
                     /translation="MLLTIDVGNTNITLGLYDGEQRKHAWRLSTVHDRMPDEYGLQLL
                     GLLEHVDCRVEQIDGCALASVVPPLNSTFLEACRRYLNLEPLVVDAGVKTGLSIRYED
                     PKHTLGADRVADAVAVRSLYGTPACVVDFGTATTFDAISAAGEYLGGAIAPGIDIAAE
                     ALFLRAAKLPRVELARPEAAIVRNTLTAIQSGLVFGYVGLVEGMVARFKHELGAGTKV
                     IGTGGLVDLIAPETKVIEFVAPWLTLDGLRIIWELNQVQ"
     gene            complement(3367..4203)
                     /gene="panC"
                     /locus_tag="VI520_06645"
     CDS             complement(3367..4203)
                     /gene="panC"
                     /locus_tag="VI520_06645"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLF37595.1"
                     /translation="MKVIRSLGELRTARAALPGLLGLVPTMGYLHDGHLSLVRRAKAE
                     CASVGVSIFVNPAQFGPTEDLDSYPVDLQRDLDLLRREQVDLVWTPDREELYPPGFQT
                     WVDLENLPQHLEGKSRPGHFRGVTTIVTKLFHTFQPDRAYFGQKDAQQVVVIRQMVRD
                     LDFPLQVVVCPTVREPDGLAMSSRNSYLSPRERAAATVLYRALTAAELVFHAGQREAE
                     SLRLRMREVLMSERRARPEYVSAADPDTLEELEGPVEAGLLSLAVLIGKTRLIDNLLI
                     GK"
     gene            complement(4215..>4864)
                     /locus_tag="VI520_06650"
     CDS             complement(4215..>4864)
                     /locus_tag="VI520_06650"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLF37596.1"
                     /translation="AKCLAPQGVALTLQNGLGNLELLEAELGPQRVALGVTTVGATLL
                     GPGKVRVGGSGATHVAPHPRLEPLVDLLQTAGFEVHPADKIESLVWGKLVVNCGINAL
                     TALLEVPNGELLARPGARAIMQKAAEETARVAEANGVKLPYPDPVEQVEGVARRTASN
                     SSSMLQDIRRGAPTEVDAINGAVVQVADRLGVPVPVNRTLWNLVRAKVQPWLEAG"
BASE COUNT          917 a         1706 c         1441 g          800 t
ORIGIN      
        1 ccgaacccgg cgtccgaggc cgtgatgtcg ttggcgacaa tcaggtcaag gtctttctct
       61 tccagcttgc tgcgggcgtt ggccagcagg tcgtcgctct cggccgcgaa tccgaccacc
      121 agcttgggcc ggcggtctcc caggctcgtc aagatgtcct cggtcggctc gagggtcacc
      181 ttcggcacgc cggcttgacg tttgatcttg tcccgctcgg cgcgagtcgg gcgaaaatcc
      241 gcaaccgccg cggccatgag cagcaccgcg gcctgaggca gggccgccga gacggcttcg
      301 gccagctgcg cggcagtatg aacatcgacc cgggccaccc cgaccggagt tccgagcgag
      361 gtaggcccgg ctatgagaac cacttcggcc cctcggtcga tcgccgcctg ggccagcgca
      421 aagccctgtt tgccagagga tcggttcgcc aggacccgga ccgggtccaa cggctcctgg
      481 gtcccgccgg cggtgaccac gaccttccgc cccgcgagcg gacccgcccg gcctagaatc
      541 cgccgaatgt gcccgagcag ctgctccggt tccagcatgc gtcccagacc gaccaggccg
      601 gaggccatcc gaccctcgac cgggcccagc agaatgactc cccgctcgcg caggatggcg
      661 agattgactt gggtagccgg atgttcgaac atccctgcgt ccatggccgg agccaccacg
      721 atcgggcagc cggcagccag ggcggtaatg ctcagcaaat tatcggcccg cccctgggcc
      781 agccgggcga ttgtgtcggc cgtggcggga gcgaccacca gcagctgggc ccgctgcccc
      841 agcccgatgt gcagcacgtg cccctcctgc gaccagagat cggaatcggt ataagctcgc
      901 cgaccggtga gcgattggaa ggtcaaccca ctcacgaact tctgggcgct ctcggtcagc
      961 acggtgtcca cctcgacccc ggcctgggtt agcctggagg cgagctcggc agccttgtag
     1021 gcggcaatcg aaccacagac gcccagaagg atccgccgcc ctttgagcag cgggacctcg
     1081 ctacttgggt ccggcaccag cttgcctctg cccggactgc tgctcgtaca tcttctgtcg
     1141 ggaaacggcc gttcggcggt aaaccaacac cccagcagta aatacggtca ccaggatcca
     1201 ccaggacatc gcgatcggcc ccgccacccc ccgccagcgt ccgctgtcca tatagaaata
     1261 caagtcgggc tcgctcgcga cgtaacggcc gcgcacctca gttacgtact gcaccactcg
     1321 gttgtagtcg tccgcgttga aatacaggta ctggaagtag tcaccgtcca cgctgagtag
     1381 gaagatatag atcaggctcg gccgcggctc gatcggcgcc ggcccgcccc gggtgcccac
     1441 cagcccggcc atgagctggc tatctccgtg ccgcagcgct tgctcgagat cgatcgccac
     1501 ggagcccggt gtttcgtacc gattgaggaa gtcctcagtg aaatacgact catcatagcg
     1561 ggtcagttcc tgcacggccc ttactccatg caaaaccaac accaccgcga tagggagcag
     1621 cagcagcccg accacatacg gccaatcgat caatcgtttc ttgttcattc gcctccgccg
     1681 ctcaccgcga ctcttcgttt cggcccaaaa cgatcccgga ataccccggc cactccggcc
     1741 aacaccaacg ccgcgccggc gagcgtcaga ggggtcagct cctccctgag caggatggac
     1801 cccagcacca gcgcgatgac cggattcacg taggcattgg tggtggccac actgattggc
     1861 agcaagcgca gggtctgaac atacgaggtg tacccgataa tggacccgaa gatcagcaga
     1921 taggcccagg ctgcccaggc ctcccgcacc ggattggcaa ccggctcggc taccactcgc
     1981 gagacaatca agaagcccaa gcccccggcc aggtgctggt aggccgagct ggcctggagc
     2041 ccgagctgaa ccttccgccg gctttgccaa actgtcccgg cggcccaggt catcgctgcc
     2101 agggccaatc ccagaatcga caagccgtca cgcggcacgc cggaggacag caccggcgcg
     2161 ctcagcaggc cgatgccggt cagggcggtg agcagcgata cgagcagcag ggccgacggc
     2221 ggccggcgat ccaggaaagc ttcgtacacg cccacccaga tcggcaccgt cccaatcatc
     2281 attgccgcca gccctgaggc cgcccgctgc tcggcccagg tcaccaaacc gttgccggcg
     2341 gtccacaaca gcaggcccga cccgatcagc accggcagtt cggccctggt cgggacgatc
     2401 cgcttgccac gcagcctggc gatcaccaat aggatcaggc ccgcggtgag cactcgggtc
     2461 gcgcccatcg tgaagggcgg aaagccggac ccctcccgca ccgcgactcg gatcgcgaga
     2521 aacgtgctgc cccacagcag gtacacaacg cctaagtgga aaagacccgt ggagctcatt
     2581 ggacttgatt aagctcccag atgatccgca ggccgtccag cgtcagccag ggggccacga
     2641 attcgatcac cttagtttcc ggcgcgatca gatccaccaa acccccggtt ccgatgacct
     2701 tggtacccgc ccccagctcg tgcttgaagc gggcgaccat tccttccacc aggccgacat
     2761 agccgaagac cagtccggat tgaatggcag tcagcgtatt gcggacgatg gccgcctccg
     2821 gtcgggccag ctcgacccgc ggcagcttgg cggcccgcaa gaacagtgct tcggccgcaa
     2881 tgtcgatccc cggtgcaatc gcccctccta agtactctcc agcagccgaa atggcatcaa
     2941 aagtcgtggc ggtgccgaag tccactacgc aggccggggt tccatagagg ctgcgtacgg
     3001 caaccgcgtc ggcaacccga tccgccccca aggtgtgctt cgggtcctca tagcggatgc
     3061 tcaatccggt cttgactccg gcatcaacga ccaggggctc cagattcaaa tagcggcggc
     3121 aggcctccag gaacgtgcta ttcaatggag gcacaaccga ggccagcgca caaccgtcga
     3181 tctgctcgac ccggcagtca acgtgctcca acagcccgag cagttgcagc ccgtactcgt
     3241 ccggcatgcg gtcatgcacg gtcgacagcc tccaggcgtg tttgcgctgc tcgccgtcat
     3301 agaggcccag ggtgatgttg gtgtttccca cgtcaatcgt tagcagcatt tgacctcgat
     3361 ctcagctcat ttgccgatca gcaggttgtc gatcaagcgg gtctttccga tcagtactgc
     3421 caatgagagc agcccggcct ccaccggccc ctcgagttcc tcgagcgtgt cggggtcggc
     3481 cgccgaaaca tactccggcc gcgcccggcg ttcagacatg aggacctccc gcatccgaag
     3541 ccgcagcgat tcggcctctc gttggccagc atggaagacg agctccgccg cagtaagcgc
     3601 ccgatacagc acggtcgcgg cggcccgctc ccgcgggctg aggtaggagt tgcggctgga
     3661 catggccagc ccatccggct cgcgcacggt tggacagacg acaacttgca gcgggaagtc
     3721 gagatcccgc accatctgcc gaatgacaac cacctgttgg gcatccttct gcccaaagta
     3781 ggcccggtcc ggttggaagg tgtggaaaag cttggtgacg atcgtcgtca ccccgcgaaa
     3841 gtgcccgggt cgcgatttcc cttccaagtg ttgggggaga ttctcaagat ccacccaggt
     3901 ctgaaaccca ggcggataca gttcttcccg gtcgggggtc cacaccagat cgacttgctc
     3961 acgcctgagc aggtccagat cccgctgtag atcaaccggg taagaatcca agtcctccgt
     4021 cggcccgaat tgggccgggt tcacaaagat gctcaccccc acactggcac actccgcctt
     4081 tgcccggcgg accagcgaca aatggccatc gtgcaggtag cccatggtgg gtaccaggcc
     4141 gagcagaccc ggcagagctg ctcgcgccgt gcgcaattca cccaagctgc gaatgacctt
     4201 cattcgattc tcgttcagcc ggcctcgagc cacggttgta ccttggcccg caccagattc
     4261 cacaatgtcc gattcaccgg caccggcaca ccgaggcggt ccgccacctg gacgaccgcg
     4321 ccgttgatcg cgtccacctc ggtcggcgca ccccgccgga tgtcctgcag catcgaagag
     4381 ctgttgctgg ccgtacggcg ggccactcct tcgacttgct ccaccggatc cgggtacggc
     4441 agcttcaccc cgttcgcctc cgccactcga gcggtttcct ctgctgcctt ctgcatgatc
     4501 gcccgagcgc ccgggcgggc gagcagctcg ccgttcggaa cctcgagcaa tgcggtcagg
     4561 gcgttgatcc cgcagttgac gaccagcttg ccccacacca ggctctcgat cttatcggcg
     4621 ggatgcactt cgaaaccggc cgtctggagc agatccacca atggttccag ccggggatgc
     4681 ggtgcaacgt gggtcgctcc cgagcctcca acccgaacct tcccgggacc aagcagggtc
     4741 gccccgacgg tcgtgacccc cagcgcaacc cgttgtggac ccaattcagc ttcgagcagc
     4801 tccaggtttc cgagcccgtt ctggagagtc agcgccactc cctgtggggc caggcatttc
     4861 gcca
//
LOCUS       DATDPQ010000297         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2510448, whole genome shotgun sequence.
ACCESSION   DATDPQ010000297 DATDPQ010000000
VERSION     DATDPQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510448"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..670
                     /gene="rsmD"
                     /locus_tag="VI520_06655"
     CDS             110..670
                     /gene="rsmD"
                     /locus_tag="VI520_06655"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HLF37597.1"
                     /translation="MPIRVVAGTAKGRRLRLVPGEGTRPVRDQVKEALFNILASQIDG
                     CTFLDLFAGTGSVGIEALSRGATHSTFVEKQPRAIETIRQNLEHTGLSDRARVIQGDV
                     FAVLEAGSRDRFDLVYVAPPQYSKLWSRAVIGLDQGRDWLNPDAWVIAQIDPKEHESL
                     ELRRLIEIDRRKYGRTMLVFYEWPGE"
     gene            complement(649..1539)
                     /locus_tag="VI520_06660"
     CDS             complement(649..1539)
                     /locus_tag="VI520_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37598.1"
                     /translation="MSRPGRSGLPVHTWAGLGILAGGQLLLRAGSAWMATWLTPVMWT
                     GVILLMDGLVFRLRGRSWIMSRGRELPLLALASVGVWLIFEAYNLHLRNWRYEGLPES
                     SLVRDLGYFWSFATIMPGVFEATDLALAILERRRSAERIPVSMMRVDRPWAWSIAGIG
                     MVTIPLALPPETAALLFGAVWIGFFLLLDPLNEMLGVPALRQRWRLGDRRLVYSLLLG
                     GMICGLLWEFWNHQAAAAGGAYWVYIFPDPLRPLGLRFGQMPVLGLLGFPPFALELFA
                     FYQFIREILGGDRVYSPGHS"
     gene            complement(1536..2036)
                     /locus_tag="VI520_06665"
     CDS             complement(1536..2036)
                     /locus_tag="VI520_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLF37599.1"
                     /translation="MSLDGQRADPDRYTLVPRTLTFLFQADQVLLLKVNRGGWAGLYN
                     GIGGHLEQGEDPLTAADRELREETGLNSIDQRLAGVVTVDTGTRPGIGLFVFVGEADP
                     ATVLSGPAGTLQWVPLTEIERLPHVQDLPFLIAHAVKSQHDGGVFFAAYRYSAEGELV
                     ISPGPS"
BASE COUNT          430 a          648 c          665 g          336 t
ORIGIN      
        1 atcatcgagg gtggttcagg catggcagcg gacaagatcg cggccctcga atcggccgga
       61 gtttcagtgg ccacgacccc tgaagcaatt cccgaactgc tgacctgaaa tgccgatccg
      121 ggtagtggcc ggcaccgcca aagggcggcg cctgcgcctg gtccccggtg agggtacccg
      181 gcccgtccgg gatcaggtca aggaagccct tttcaacatt ctggcctccc agatcgacgg
      241 ctgcaccttc ctggatctat tcgccgggac gggcagtgtt gggatcgagg cgctgagccg
      301 gggagcgacc cactccacgt tcgtcgagaa gcagcctcgg gcaattgaga cgattcgcca
      361 aaatctcgag cacaccgggt tgagcgaccg ggcgcgagtg attcaaggtg acgtgttcgc
      421 ggtgctcgag gcgggatccc gggatcggtt cgacctggtt tatgtggccc cgccccaata
      481 ttccaagctc tggagccggg cggtgatcgg cctagaccag gggagggact ggttgaatcc
      541 ggatgcctgg gtgatcgccc agatcgaccc gaaggaacac gaatcgctcg agcttcggcg
      601 gttgatcgag atcgaccggc gcaaatacgg ccggacgatg ctcgtgttct atgagtggcc
      661 gggagagtag acccgatcgc cacccaggat ctctcggatg aattgataga acgcgaatag
      721 ctctagcgcg aacggaggga aaccaagcag acccaacacc ggcatctgcc cgaaccgcag
      781 gccgagcggt cgcagcggat caggaaagat gtagacccag taggcgccac ctgccgcggc
      841 tgcctggtgg ttccagaact cccaaagcaa cccgcagatc atcccgccga gcaggagcga
      901 gtacacgagt cgccgatcgc ccagccgcca gcgctggcgc agcgccggta ctccaagcat
      961 ctcattcagt ggatcgagca gtagaaagaa gccaatccag accgcgccga acaacagcgc
     1021 ggcggtttcg ggaggcaggg ccagcgggat cgtgaccatt ccgatcccgg cgattgacca
     1081 ggcccacggg cgatcgactc tcatcatcga caccggaatt cgctcggccg atcggcgtcg
     1141 ctccaggatc gccagagcca aatcggttgc ttcgaagact cccggcatga tcgtggcgaa
     1201 cgaccaaaag taacccagat cgcggaccag gctgctttcg ggcagccctt cgtaacgcca
     1261 attgcgcaga tgcaggttgt aggcctcgaa gatcagccac acgccgaccg aggcgagggc
     1321 gagcaacggg agctctcgtc cgcggctcat gatccaggag cgaccgcgca agcggaagac
     1381 aagcccgtcc atgagcagga tgaccccggt ccacatcacc ggagtcagcc aggtggccat
     1441 ccaggcactg ccggctcgca gcagcagctg tccgcccgca aggatcccca gtccagccca
     1501 ggtgtggacc ggcagacccg acctgcctgg tctgctcaac tagggcctgg agaaatcacg
     1561 agttcgccct cggccgagta ccggtaagcc gcaaagaaca cccccccatc gtgctgagac
     1621 ttgacggcgt gggcgatgag gaatggaaga tcttgcacgt ggggcaggcg ctcgatttcg
     1681 gtcagcggga cccattgcag agttcccgcc ggaccggaaa gaacggtggc cggatcagcc
     1741 tcgcccacaa agacaaagag tccgatccct ggccttgtcc cggtgtcgac cgtgaccacc
     1801 ccggccagcc gctggtcgat cgagttcagg ccggtctctt cgcgaagctc gcggtccgcg
     1861 gcggtcaagg ggtcttcacc ttgctccagg tgtcccccga ttccgttgta aaggccggcc
     1921 catccgcccc ggttcacctt gagcagcaga acttggtcgg cttggaagag gaaggtgagg
     1981 gtgcgaggta cgagcgtata tcgatcggga tccgctcgct gcccgtccag ggacatcggc
     2041 ggcgaattat actcttggca tggttctgta ggagggtag
//
LOCUS       DATDPQ010000298         6418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1480736, whole genome shotgun sequence.
ACCESSION   DATDPQ010000298 DATDPQ010000000
VERSION     DATDPQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480736"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VI520_06670"
     CDS             <1..318
                     /locus_tag="VI520_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLF37600.1"
                     /translation="EAAAQFEGRVEVLDLRTISPWDQEAVLVSVRKTGRCLVVHEDGW
                     TAGFGAEILATLGEAAFFELDAPLRRVAVRDLPIPYSAGLMSAVLPNTEQLAAAIADL
                     LAF"
     gene            345..2171
                     /gene="pepF"
                     /locus_tag="VI520_06675"
     CDS             345..2171
                     /gene="pepF"
                     /locus_tag="VI520_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HLF37601.1"
                     /translation="MAIEVRERTQIPKQFTWNTESVFATPQDWESEFKALGADLEGLG
                     QHHGKLSEGPSTLLAALELLQQLLARAWKLVIYAMFSYSVDTRQQEAAQRQGRAEGII
                     AQATAAGAFVNPELLSIGEPKLREWIRSDPRLMLYDHYISNLFRIQQHVRSAEVEELL
                     GRVLDPFKAVYTTVSTLVDSDFQFRAARSAEGREIPVTQGSVDTILRDPVRELRRTAW
                     ESYADTYLGFKNTLANNLAASIRQNVYLMRARRHESSVGMALFKDNISEPVFHNLIET
                     FRKHVGTWHRYWKVRRAALGVEQLHPYDVWAPLTDSPPTISYQQAVDWVCEALTPLGE
                     EYVERMRRGCLEQRWVDVYPNLGKHQGAFSYGSPGTYPFILMSFADDVSGLSTLAHEL
                     GHSMHSYLAWKHQPMIYSEYSLFAAEVASNFHQAMVRAYLLKNNADVQLQISIIEDAM
                     DNFHRYFLIMPTLARFELEIHQRVERGEGFSADGLIELMADLFAEPFGSEMRLDRQRV
                     GITWAQFLHLYQDYYVYQYATGISGAHSLAARILAGETGAAEAYLEFLKAGASVYPLD
                     ALRGAGVDLGSPEPVEQTFKVLDDLVDRLAKLTGVAEAVAPA"
     gene            2191..3342
                     /locus_tag="VI520_06680"
     CDS             2191..3342
                     /locus_tag="VI520_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLF37602.1"
                     /translation="MKLEFQEYRPRRIVNVRKHVDGPWFWDKYSAHPYIGCRSGCEFC
                     YLRGSRYLGRRDPESFDTLIQVKSNAAELLRKELARLEPDVIACGDWQQPAEDRYRLS
                     RSLLEVVLELGFPLFVVERSPLLARDLDLLVEIHRRSWVGLVFSISNLDPALKQAFEP
                     RSPGVQRRLEAMRQLADAGLLVGTTLMPIIPAAGDRAPQLEEVVVATRDHGGSFVLGG
                     GLSMEGVQADRTLEAALRLDLGLERPLRRLYGWQPEGVPRYGPPREYAARLGRMVREL
                     CEKHGLRDRMPRYIPAGPLAVNKRLAEQLFLKTYDLELERAATQAVWAFRKAAWAVDE
                     LASRVDDLYRGQGLAGLESVAGIGPQPAEFAASWLQQDDRDKTAARLVE"
     gene            complement(3378..4880)
                     /locus_tag="VI520_06685"
     CDS             complement(3378..4880)
                     /locus_tag="VI520_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLF37603.1"
                     /translation="MSPPKISLLHLIWLVLLAAAVSGCAAAQDTSKAYSRSAISRGAI
                     VPADTIRLHEYLNYYEQRFPEPIDQPLGLDLRLGNSQIPAAGGLVWLQIGLQAQSGEL
                     AVRTPLNLALVLDTSGSMDSPDKMPFLQQSLGVFLGSLQPEDQVALVTYSDEADLLWP
                     SSPVGDGRWIRGAIESLRPGGSTNLHAGLMLGLAEVDRQFDIRRNNRVILLTDGIANQ
                     GVTDSSEIAREALAFNQRGIYLSTIGLGLDFNDELLATLAQQGRGAYHFVDSAQEMDK
                     IFRQEVEGLVESAANDVRVALRTMPGVRLISITGYEGTPPQSGAEVNLQDMGSGNSQV
                     LMARLQVDPGLPGSRPLAEVTLRYEDAFAQRARETSATLVAQAAELFGYDPLTDVEVQ
                     RNVTIVQSAEALIAIDGLFNQGRYAEAWQLAYEMEQELRWLAALAADQQLVQDADLFA
                     RYQLTLVSALGYTPSLYEPTSVPPIGGQDQRWGSTAVPAYPPLPTLEVDR"
     gene            4997..>6418
                     /locus_tag="VI520_06690"
     CDS             4997..>6418
                     /locus_tag="VI520_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37604.1"
                     /translation="MKSFGELLSEYINQTGVSDAELARRLGISRQTVFRWREGLTQRP
                     RHRQDVLRMAEMLRLSQPERDQLLLAGGFPPVQPQQAAGQPAEGSKAAGSGEEMPGRP
                     PPIGVSVRVTSPGLEAWQTLWQRWRIAGLGLVVVLVIGVGFPTGFWAQLAVGLGLAQS
                     GPDGLVQPAEPGETLVLISPFANYSGGQAGFNVAGRLREALELEFEQAELDGVRVEVP
                     SASVQDEAAALELGDQLNAVLVVWGEYDSGRVVAVVTGAGGEPPSDGQQRRWLITSPG
                     ELSTTINSELSDEVRWMALYILGQSHFQAGRLVEAEAAFNRALASPPRDPETQAVVFY
                     YLALLESKEESPDQSKVVALYSEALELRPELASAFNNRAVAYINRESPGDLERAEADL
                     QAALSQEPQDPTLLFNLALTTARQGPEHLAETLDLLGRAEQLKPESPGIQNGLCWFYS
                     LAGSPQQALPYCDQAVQLDTSGLS"
BASE COUNT         1135 a         1926 c         2146 g         1211 t
ORIGIN      
        1 gaggcggcgg cacagttcga aggccgggtg gaggtgctcg acctgcgtac gatcagccct
       61 tgggaccaag aagcggtgct ggtatcggtg cgcaagaccg gccgctgtct ggtggtccat
      121 gaggacggct ggaccgccgg cttcggcgcc gagattctgg ccacccttgg ggaggcggcg
      181 ttcttcgagt tggacgcgcc gctgcgccgg gtggcggtcc gcgacctgcc gatcccctac
      241 agcgccggcc tgatgagcgc agtgcttcct aacaccgagc agctcgctgc ggcaatcgca
      301 gatctgctcg cattctagaa tcaacccgtc agcgagagag gaacatggcc atcgaagttc
      361 gagaacggac ccagatccct aagcaattca cctggaacac cgagagtgta tttgccactc
      421 cgcaggattg ggagtctgag ttcaaggcac tcggcgcgga cctggaaggc ctgggccagc
      481 atcacggaaa actgtcggaa gggccttcta cgctgcttgc cgctctcgaa ctgctgcaac
      541 agctgcttgc ccgggcctgg aaactggtga tctacgcgat gttttcgtat tcggtcgata
      601 cccgccagca ggaagccgcc cagcgacagg gtcgggctga aggcatcatt gctcaggcga
      661 ccgcagccgg cgcattcgtc aacccggagc tgctttcgat cggcgagccg aagctccgcg
      721 aatggatacg aagtgatccg cggctgatgc tctacgatca ttacatcagt aacctgttcc
      781 gcatccagca gcatgtccgt tctgcagaag tcgaggaact gctcggcagg gtgttggatc
      841 ccttcaaggc ggtctacacc accgtttcga ccctggtcga ttctgacttc cagtttcgag
      901 cggcaaggtc ggcggaaggt cgggagatcc cggtgaccca aggtagcgtg gacacgattc
      961 ttcgggatcc ggttcgggaa ctccggcgga ccgcctggga gagttatgcc gacacctacc
     1021 tgggcttcaa gaacaccctg gcgaacaatc tggcggcctc catccggcag aatgtgtatc
     1081 tcatgcgcgc ccgacggcac gagtccagcg taggaatggc gcttttcaag gacaacatct
     1141 ccgagccggt cttccacaat ctgattgaga cttttcggaa gcacgtcggt acctggcacc
     1201 gctattggaa ggtccgccga gcggcactcg gggtcgagca actccatcct tacgatgtat
     1261 gggcgccgtt gaccgacagt ccgccgacca tcagctatca acaggcagtg gactgggtgt
     1321 gcgaggcctt aacgcctttg ggggaggaat acgtcgagcg gatgcgcagg ggctgcctgg
     1381 agcagcgctg ggtggacgtg taccccaacc tcggcaaaca ccagggagcc ttctcctacg
     1441 gttcgcccgg cacgtatccg ttcatattga tgagcttcgc ggatgatgtc tccggcctca
     1501 gcaccctggc ccatgagctc gggcactcga tgcactcgta tctggcctgg aagcaccagc
     1561 caatgatcta ctccgaatac tcgctcttcg cggccgaggt ggcctcgaat ttccatcagg
     1621 cgatggtgcg ggcgtacctg ctcaagaaca acgccgatgt ccagctgcag atttcgatca
     1681 tcgaggacgc gatggacaac ttccaccgct acttcttgat catgcccacc ctggcccgct
     1741 ttgagttgga gatccaccag cgcgtggaaa ggggtgaggg tttcagcgca gacgggttga
     1801 tcgagttgat ggccgatctg ttcgcagagc cattcggatc cgaaatgcgg ctcgaccggc
     1861 agcgggtcgg gatcacctgg gcccagttcc tccatctgta tcaggactac tatgtctatc
     1921 aatatgccac cgggatctcc ggggcgcact cgctggccgc ccgcattctg gcgggggaaa
     1981 ccggcgcggc ggaggcctac ctggaattcc tcaaggccgg cgcctcagtc tatccgttgg
     2041 acgcactgcg gggagccggc gtcgacttgg gctcgccgga accggtcgag cagaccttca
     2101 aagtgctcga cgatctggta gaccggctgg cgaagctcac cggcgtggcc gaggctgtgg
     2161 ccccggccta gttcgagtcg attcgccccg atgaagctcg aattccagga gtaccggccg
     2221 cgccggattg tcaacgtccg caagcacgtg gacggcccct ggttttggga caagtacagc
     2281 gcccatccct acatcggctg ccgcagcggt tgcgagtttt gttacctgcg cggttcacgc
     2341 tatctgggcc gccgagaccc ggagagcttc gacaccctga tccaggtcaa gtccaacgcc
     2401 gccgagttgc tgcggaaaga gctcgctcgg cttgaaccgg atgtgatcgc ctgcggggac
     2461 tggcagcagc cggcggaaga tcgttaccgc ctctcccgct cactcttgga agtcgtgctg
     2521 gaacttggat tcccgctgtt cgtggtggag cgttccccgc tgcttgcccg cgatctggat
     2581 ctgctggtcg agatccaccg gcggtcatgg gtggggctgg tgttcagcat cagcaatctc
     2641 gatccggcgc tgaagcaggc cttcgagccg cgcagcccgg gtgtccagcg tcggttggag
     2701 gcgatgcgac agctggcgga cgccggcctg ttggtgggca ctaccttgat gccgatcatt
     2761 ccggccgcgg gcgaccgtgc gccccaactt gaagaggtgg tcgtcgcaac tcgcgatcac
     2821 ggtggatcct tcgtgctggg cggcgggctg agcatggagg gggtgcaggc cgaccgcacg
     2881 ttggaggctg cgctgcgtct ggatctcggg ctcgagcggc cgctgcgccg cctgtatggc
     2941 tggcagccag agggcgtgcc gcgctacggg ccgccgcggg aatacgccgc caggctggga
     3001 cggatggtgc gcgaactgtg tgagaagcat ggcctgcggg atcggatgcc gcgctacata
     3061 ccggccggac cgctggcggt caacaaacgg ttggccgagc agctcttctt gaaaacatat
     3121 gatctggagc tggaacgggc ggcgacccag gcggtctggg ccttccgcaa ggcggcctgg
     3181 gcggtcgatg agcttgcgag tagggttgac gacctctacc ggggccaagg actggctgga
     3241 cttgagtccg tggccgggat cggcccccag ccggccgagt tcgccgcgag ctggctgcag
     3301 caagacgatc gcgacaaaac cgcagcccgc ctcgtggagt gatccggcct tgaggcgggc
     3361 tgcgggttca acttgggtca gcgatcgact tcgagggtgg gaagtggggg gtacgcgggg
     3421 accgcggttg acccccagcg ctggtcctgc ccgccgatcg gtggaactga agtcggctcg
     3481 taaagtgaag gtgtgtaacc aagcgcagat accagggtca gctggtagcg ggcgaagaga
     3541 tcggcgtcct ggaccaactg ttggtcggca gccagcgccg ccagccatct caactcctgc
     3601 tccatctcat aggccagctg ccaggcttcc gcgtagcggc cctggttgaa caatccgtcg
     3661 atggcgatca gcgcctccgc cgactggacg atagtgacat tgcgctggac ctcgacatcc
     3721 gtcagcggat cgtagccgaa caactcggcg gcctgggcga cgagggttgc actggtttcc
     3781 cgggcgcgtt gggcgaaagc gtcctcgtac ctcagtgtga cctcggcgag cggacgcgag
     3841 ccaggcaggc ccggatcgac ctgcagcctg gccatcagga cctggctgtt tccgcttccc
     3901 atgtcttgca ggttgacctc ggcgccggat tggggcggag tcccttcgta accggtgatg
     3961 ctgatcaggc gcactccggg catggttcgc aaggcgaccc ggacatcatt ggccgcgctt
     4021 tcgaccaggc cctcgacttc ctgccggaag attttgtcca tctcctgggc cgaatcgaca
     4081 aagtggtaag cgcctcggcc ctgctgggcc agggtggcga gcagttcgtc gttgaagtcc
     4141 aagcccagtc cgatggtcga tagatagatc ccgcgctggt tgaacgccag ggcttcgcgg
     4201 gcgatctcgg aggagtcagt gacgccctgg ttggcaatgc cgtcggtgag caggatgacc
     4261 cgattgttgc ggcggatatc aaactgccgg tcgacctcgg caaggcccag cataaggcct
     4321 gcgtgcaggt tggtgctgcc tcccggccgc agactctcga tcgccccgcg gatccagcgg
     4381 ccgtcgccga ccgggctcga tggccagagc agatccgcct catcgctgta ggtgaccagg
     4441 gcgacttgat cctcaggttg caagcttccc aggaacaccc ctaacgactg ctgcaggaag
     4501 ggcatcttgt ccgggctatc catcgaaccg ctggtgtcga gcaccagggc gaggttgagc
     4561 ggagtgcgaa ccgccagctc accgctctgg gcctgtagcc cgatctgcag ccaaaccagg
     4621 ccgccggcgg ccgggatctg cgaattgccc aatcgcaggt cgaggccgag cggctgatcg
     4681 atcggctccg ggaaccgctg ctcgtagtag ttgaggtatt cgtgcaagcg gatggtgtcg
     4741 gccggcacga tggcgccgcg actgatcgcg gaccggctgt aggccttgga ggtatcctgg
     4801 gcagccgcgc atccgctgac ggcggcggcc aggagaacta accagatgag atggaggagc
     4861 gagatcttgg gaggggacat tgggtactcc tttccttagt tggatttgcg ttcctgtccc
     4921 atactgcctg aaggtcgatg catttcaatc ggcagctgtg ttacattcgc tgcaacaggc
     4981 gtagcatccg cttcgaatga agtccttcgg cgagctgctc tccgagtaca tcaaccagac
     5041 cggcgttagt gacgcggaac tggcccgccg actggggatt agccggcaga ccgtgttccg
     5101 ctggcgggag gggctgactc agcggccccg acaccggcaa gacgtgctgc ggatggccga
     5161 gatgctccgg ctctcgcagc cggaacggga tcagctctta ctggccggcg gattcccacc
     5221 ggttcagccc cagcaggcag ccggccagcc ggccgaggga agcaaggctg caggatctgg
     5281 ggaggagatg ccaggccggc cgccgcccat tggcgtttca gtccgggtta catcgccagg
     5341 gctggaagct tggcagaccc tctggcagcg gtggcggatc gcaggtcttg ggctggtagt
     5401 tgtgctcgtg atcggggtcg ggtttccaac tggcttttgg gcacagctcg ccgtgggtct
     5461 cggactggcc caatccggac cggatgggct tgtgcaacca gctgagccag gtgagacgct
     5521 agtgttgatc agtccgttcg caaattacag cgggggtcag gcgggattca acgtggcggg
     5581 ccggttgagg gaagccctcg agctggagtt tgagcaggcc gagctcgatg gggttcgggt
     5641 cgaggttcct tctgccagcg tccaggatga ggcggccgcc ctggaactag gtgaccagtt
     5701 gaatgctgtg ctcgtggtgt ggggcgagta cgacagcggg cgggtggtcg cggtggtgac
     5761 gggggcgggc ggcgagcctc cctccgatgg tcagcagcgg cgttggttga tcacctcacc
     5821 aggcgaactc agtaccacga tcaattccga gctgtctgac gaggttcgct ggatggccct
     5881 gtacatcttg gggcaatcgc actttcaggc tggcaggctt gtggaggccg aggcggcctt
     5941 caatcgggcg cttgcgtctc ccccacgaga cccggagacc caagcggtgg tcttttacta
     6001 ccttgcactg ctcgagagca aagaggagtc cccggatcag agcaaggtcg tcgcgctgta
     6061 cagcgaagca ctcgagctga ggccggagct ggcctcggcg ttcaacaatc gtgcagtggc
     6121 ctatatcaac cgtgagtctc ctggggactt ggaacgggcc gaggctgact tacaggccgc
     6181 gctcagtcaa gagcctcagg atcccaccct cctattcaat ctggccctca caacagcccg
     6241 ccaggggccg gagcatcttg cggagacgct cgatctgctg gggcgggcag agcagctgaa
     6301 gcccgaatcg cccggcatcc agaacggctt gtgttggttc tattcgttgg ccggcagccc
     6361 gcagcaggct ctaccgtact gcgatcaagc cgttcagctt gatacatcgg gtttgagc
//
LOCUS       DATDPQ010000299         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_938817, whole genome shotgun sequence.
ACCESSION   DATDPQ010000299 DATDPQ010000000
VERSION     DATDPQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938817"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..784
                     /locus_tag="VI520_06695"
     CDS             122..784
                     /locus_tag="VI520_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HLF37605.1"
                     /translation="MHTQAVEDYLKNIYELQASAEKVSTTDLAERLGVAPASVTGMVK
                     KLAAMKLLKHEPYHGVLLTKAGEKIALEVIRHHRLVESYLTEALGVPWDKVHEEADKW
                     EHVLSEEMEDRISAMLGDPTTDPHGAPIPARDGSLPKTSLRRLSDLEPGERAVVAEVS
                     DHDPELLRYLGEMGLFPQTKLRLISAAPFQGPLTIEIGGREQAVGREVAGQVLVESGV
                     KA"
     gene            905..1477
                     /locus_tag="VI520_06700"
     CDS             905..1477
                     /locus_tag="VI520_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLF37606.1"
                     /translation="MNEKNEADLISQARAGEAAAWEALVHNHEERVFRLAYLMLADPD
                     EAQDVAQDTFIRAYQHLKNFDDQRPLQPWLLRIASNLARNRRRSIGRYWFALQRLARP
                     GLEIQRTTEGAALNREGLEALWRAVRRLSEPDQRVIYLRYFLELSEAETAAALEVPAG
                     TVKSRLHRALVRLRTIIEREFPELREGLYA"
     gene            1474..>2081
                     /locus_tag="VI520_06705"
     CDS             1474..>2081
                     /locus_tag="VI520_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37607.1"
                     /translation="MTDVDQLERFAEQIRGLAAGFNYPPTPDIAAGLRPRWGRPARRP
                     RLAWAAAIVLVIVAGLLAVPAVRAAVVEIVKLGAVRIFLGGPTPTPLPPTGHLSLSEL
                     PGETTLDAARASAAISIRLPSYPADLGPPDRVFLLREFGGIVILIWEDPNYPDLARLA
                     LFEIPPGPIVNKLFPERIQSTSVNGRPAVWATGDHFVEVIME"
BASE COUNT          381 a          658 c          651 g          391 t
ORIGIN      
        1 ctcgttcgag ccgcactcga atgcttatct gggcgcgctt tgcttgcggc cgcttgactt
       61 ccgcggtccc acggggtata cctgattagg ttttaggttt gcctaatttt ctgcggagct
      121 gatgcacacc caagcggttg aagactactt gaagaatatt tacgagctgc aggcctcggc
      181 cgagaaggtc tccacgaccg acctggccga gcggctgggc gtcgcgccgg cctcggtgac
      241 cgggatggtc aagaagctgg cggcaatgaa gctgctcaag catgagccct accacggcgt
      301 tctgttgacc aaagccggag agaagatcgc cctggaggtg attcgccatc accggctggt
      361 cgaaagctac ctgaccgaag cccttggcgt gccttgggac aaggtccacg aagaagccga
      421 caagtgggaa catgtgctct cggaagaaat ggaagaccgg atctcggcca tgctcggcga
      481 cccaacgacc gacccccacg gagcccccat tcccgcccgg gatggttcgc ttccgaaaac
      541 gagcctccgg cgcctctcgg atctcgaacc gggcgagcgg gcggtggtcg ccgaggtcag
      601 tgaccacgac cccgaattac tgcgctatct gggcgagatg ggtttgttcc cccagaccaa
      661 gctgcggctg atttccgcgg cgcccttcca gggtccgcta accatcgaga tcggcgggcg
      721 ggagcaagcc gtcgggcgag aagtcgccgg gcaagtcttg gttgagtccg gcgtcaaggc
      781 ataagcctgg tcaaagccct cctcgccggc ctttagatcc cgttcgaact gcaccctcca
      841 tagtatcgcc ccaagcctcg ggtaaggaac cgtccgcggc tgatcggtgt attgttctgc
      901 gtaagtgaat gaaaagaatg aagcggacct gatctcccag gcgcgggccg gagaggccgc
      961 agcctgggaa gcgctcgtcc acaaccatga ggagcgtgtg tttcgacttg cctatctgat
     1021 gctcgccgac cccgacgaag cccaggacgt ggcccaggat actttcatcc gcgcctacca
     1081 acacctcaag aattttgacg atcaacgccc gctgcagccc tggctgctgc ggattgcctc
     1141 caatctggcc cgcaatcgcc gccgatcgat cggccgttac tggttcgcct tacagcgcct
     1201 tgcgcggccc ggcctggaaa tccagcggac cacagagggg gcggccttga accgggaagg
     1261 gctcgaggcg ttgtggcggg ccgttcgccg gctgagcgag cccgatcagc gggtcatcta
     1321 cctgcgttac ttcctggagc tctcagaagc ggaaaccgct gccgccctgg aagttccggc
     1381 cggaactgtc aaatctcgac tgcatcgagc gctggttcgg ctgcgcacca tcatcgagcg
     1441 agaattcccg gaattgcggg aagggctcta cgcatgaccg acgtcgatca gttggagagg
     1501 tttgcagaac agatccgggg gctggccgcc ggattcaact atccgcccac gccggatatt
     1561 gcagccggat tgcgaccgcg atgggggcgg ccggcccgac ggcctcggct cgcttgggct
     1621 gcagcaattg tgctggtgat tgtggcaggt ctgctggccg ttccggccgt gcgggccgcg
     1681 gtggtcgaga tcgtgaagct cggtgcggtc cggatcttcc ttggcggccc aacccctacc
     1741 ccacttccgc cgaccggaca cttgagcctg agcgagcttc ccggagaaac cacgctggac
     1801 gctgcccggg cttcggccgc catctccatc cgtctcccca gttacccggc ggacctcggc
     1861 ccacctgacc gcgtgtttct gctgagagaa ttcgggggga ttgtcatcct gatctgggaa
     1921 gatccgaact accccgacct cgctcgcctg gcgcttttcg agatcccgcc cgggccgatc
     1981 gtgaacaaac tattcccaga acgcatccag tcgacgagcg tgaacggtcg gccggcggtc
     2041 tgggcgaccg gtgaccattt cgttgaggtg atcatggaaa g
//
LOCUS       DATDPQ010000300         4320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_940508, whole genome shotgun sequence.
ACCESSION   DATDPQ010000300 DATDPQ010000000
VERSION     DATDPQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940508"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VI520_06710"
     CDS             <1..646
                     /locus_tag="VI520_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HLF37608.1"
                     /translation="AHEFGHAIHSMLAEHHSALTQESSLPLAETASTFAEMLVVDQLL
                     ATDPDPELQRDLLFRQMDHAYASITRQAYFAVFEREAHQRVKDGGSVDDLTEVYFNNL
                     KEQFADSVDLSEDFRNEWLRVGHFFFAPFYVYAYSFGQLLVFALYQQYKAEGEPFKPR
                     YLEILAAGGSDSPVRILDRAGINVRSAEFWRGGFQVLEEALERLTSLEVTAGVG"
     gene            650..2347
                     /locus_tag="VI520_06715"
     CDS             650..2347
                     /locus_tag="VI520_06715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HLF37609.1"
                     /translation="MLPGSALEFLEWDWSRVESHYRGLRSRNLSGTNIAAWLADWSRL
                     KELIEEVQSRLYVAKTLDTADPQREQRYQAYLKEIYTPAEEQDQKLKEMLLASGMEPD
                     GFAIPLRNLRAQAELYRDANLPLLEQEKKLSSDYDRIRGSQTVEWEGEEITISRLFLE
                     LEEPDRGRRERAWRLGRARQLQDRETLNDLWVRFLDLRTGLARNADLAEYRSYRWRQL
                     LRFDYTPEDCVRFHEAIEAVVVPAAAKVADRRKRVLGIASLRPWDASADKYGREPLRP
                     FAAVEQLIDKGSAIFHQVDPVLGRYFDSMQSEKLLDLDNRKGKAPGGYNEPFAAARRP
                     FIFMNAVGTAGDVETLLHEAGHAFHVFESASLPYYPQLQVPMEFAEVASMAMELLASP
                     FLTTDHGGFYTDEQAARARIDHLERILWFWPFMAVGDAFQHWVYEHPEAARDPANCDQ
                     AYADLVGRFIPFIDWSDLSEELRTGWQRVLHFFQVPFYYVEYGLAQLAAVQIWKNALE
                     DQAGAVRRYRGALALGYTVPLPELYQAAGATFAFDAEDLSEAVALIESTVERLERRV"
     gene            2344..>4320
                     /locus_tag="VI520_06720"
     CDS             2344..>4320
                     /locus_tag="VI520_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37610.1"
                     /translation="MNVRRAARSMARFLGLAAGVLAVGGLASPAQAQSAAGFSEFYIP
                     GETQQMWNIFENLDNDPNLVEASGMHNVTAVTATLDNTTIYYDHWEDGYDFDPANPAA
                     TADESYVLNSGGVREFESSNIPVSPRGTATFYDGRDRIYVAGGPVAVTGAHWPESIGT
                     VFSLAWEIYPTKPFLTNYTIPVGQNLAGAPTNYIDFTRTYVIVQSTADGTLVQIDDPP
                     VGVDVSVTLNRGEVTQLYSIDSGTTVVASAPVQVQFIVGRFATGGTSEIDGYSVVPDS
                     LWDNEYYSPVGSAAVGGGDVDLFLYNPNASAITISFEDLTGSGSFSVPALSTRSYSDP
                     LGAGRLVPNNSGVYLSSSSIFWGIGSGDAESANWDWGYSLVPVNALQDHYFLGWAPGT
                     SQAVPTSNGSPAYVTPVQDDTTVFIDYSPTNGTVDATFTLNRLQTQRVFDPDNENTGM
                     HIFATGDIAVAWGEDPNVAATGTPYLDLGYSTLPFPPEWMDLVLGIEKTANPTSLPQA
                     AGQISTFTLVVSTFTFPVDDVDVFDTLPPGWVYVAGSTTITLPDNSTITGNAADPTTI
                     VGQVLTWDLNQDIPIAEALTVVFQAQTTAGVSGGLNQNDGEASGTRLGGAQVFAPIDS
                     AFVFLSPTGLSIDKDTSTATVDPGTTASYSIIVAN"
BASE COUNT          804 a         1353 c         1344 g          819 t
ORIGIN      
        1 ggcccacgag ttcggccacg ccattcactc gatgctcgct gaacatcact cagcgttaac
       61 ccaggagtct tccctgccgc tggccgaaac cgcttcaacg tttgctgaaa tgttggttgt
      121 ggatcagttg ctggcgaccg atcccgatcc cgagctgcag cgggacctgc tctttcggca
      181 gatggatcac gcctatgcca gtattacccg ccaggcctat tttgcagttt tcgagcggga
      241 ggctcatcag cgggtgaagg atggaggctc ggtggatgat ctgaccgagg tctatttcaa
      301 caacctgaag gagcagttcg ctgattcggt cgatctcagc gaggatttcc ggaacgagtg
      361 gctgcgagtc gggcatttct tcttcgctcc cttctatgtc tacgcctatt ccttcggcca
      421 gttgctggta ttcgctctct accagcagta caaggcggaa ggcgaaccgt tcaaaccgcg
      481 ctacttggag atcctggccg ccggcggatc cgattcaccg gtccggatcc tggatcgggc
      541 cggaattaac gtgcgatcgg cggaattctg gcgaggaggc ttccaggtcc tagaggaggc
      601 gctcgagcgc ctgacgagcc tggaggtcac ggccggggtc ggctgatcga tgctcccagg
      661 cagcgcgctc gaattcctgg aatgggattg gagccgggtc gaatcgcact atcgagggtt
      721 gcgttcccgg aatctgtccg gaacgaacat cgcggcctgg ctggcggatt ggtcgcgcct
      781 gaaggagctg atcgaagagg tccaatcgcg cctgtatgtg gccaagaccc tcgatacggc
      841 cgatccgcag cgcgagcagc ggtaccaggc gtacctgaag gagatctaca ctccggcgga
      901 ggaacaggac cagaagctta aggagatgtt gctagcgagc ggtatggagc cagatggatt
      961 cgcgatcccg ctgcgcaacc tgcgggccca agccgagctg tatcgggatg ccaacctgcc
     1021 cctgctcgaa caggaaaaga agctcagctc cgactacgac cggatccgcg gtagccagac
     1081 tgtcgaatgg gagggcgagg aaatcaccat ctcgcgcctc ttcctcgagc tcgaggagcc
     1141 ggatcgaggg cgacgggagc gggcctggcg gctggggcgg gcccggcagc tacaggaccg
     1201 ggaaacgctc aacgatctct gggtccgctt cctcgatctg cggaccgggc tcgcccggaa
     1261 cgccgacctg gcggaatacc gttcttaccg ctggcggcag ttgcttcgat tcgactacac
     1321 tcctgaggac tgcgttcgct ttcatgaggc gatcgaagca gtggtcgtac cggccgcggc
     1381 caaagtcgcg gatcggcgaa aacgggtcct ggggatcgct tccctgcggc cgtgggacgc
     1441 cagtgccgac aagtatggca gggaaccgct gcggccgttc gccgcggtcg agcagctcat
     1501 tgacaagggc tccgcgatct ttcaccaggt cgatccggtt ctgggccgct acttcgactc
     1561 gatgcaatcc gagaaactgc tggacttgga taatcgaaag ggaaaggctc cgggcggata
     1621 caacgagccc ttcgcggctg cccggcggcc gttcatcttc atgaacgcgg tcggcactgc
     1681 cggagatgta gaaaccctgc tgcatgaggc cggccatgcc tttcatgtct ttgaatcggc
     1741 gtcgctgccg tactaccctc aactgcaggt gccgatggaa tttgccgagg tcgcttctat
     1801 ggcgatggag ctgctggcct cacctttcct gacaaccgat catggcggct tctacaccga
     1861 tgaacaagcc gcccgagcgc gcatcgacca tctggagcga atcctttggt tctggccatt
     1921 catggccgtc ggcgacgcat tccagcactg ggtctacgag cacccggagg cggcccgcga
     1981 cccggccaat tgcgatcagg cctacgcgga tctggtgggc cggttcattc cattcattga
     2041 ctggagcgac ctgagcgagg aactgcggac cgggtggcag cgggtcctgc acttcttcca
     2101 agtcccgttc tactatgtcg agtacgggct ggctcagctc gccgcggtcc agatctggaa
     2161 aaatgcgttg gaggatcagg ctggcgcggt gcggcggtat cggggtgcgc tggcgctggg
     2221 gtacaccgtt ccgttgccgg agctctacca ggcggccgga gccacgttcg ccttcgacgc
     2281 cgaagacctt agtgaagcgg tcgccctgat cgaatcaacc gtcgagcggt tggagcggcg
     2341 ggtttgaacg ttcgccgagc ggcgcgatcg atggcccgct tccttggcct tgcggcaggc
     2401 gtgctggcgg tcggcgggtt ggcgagtccc gcgcaggccc agagcgcggc cgggttttcg
     2461 gagttctaca tccctggcga aacccagcag atgtggaaca tcttcgagaa cctcgacaac
     2521 gaccccaacc tggtcgaggc ctccgggatg cataacgtaa ccgccgtcac ggccaccctg
     2581 gacaacacca cgatctacta tgaccactgg gaggatggct acgactttga cccggccaac
     2641 ccggcggcga ccgcggatga gagttatgtc ctcaactccg gcggcgtccg agagttcgag
     2701 agcagcaaca tcccagtcag tccgcgcggc accgcgacgt tctacgacgg gcgggatcgg
     2761 atctatgtgg ccggcggccc ggtcgccgtc accggagcgc actggccgga atcgatcggc
     2821 acggtcttct cgctggcctg ggagatttac cctaccaagc cgtttctgac gaactacacc
     2881 attccggtgg ggcagaacct ggccggggcg ccgaccaact atattgactt cacccggacc
     2941 tatgtcattg tccagtccac cgctgatggc accctcgttc agatcgatga cccaccggtg
     3001 ggggtagacg tgagcgtcac cctcaaccgg ggtgaggtca cccagctcta cagcatcgac
     3061 tcgggcacca ccgtggtagc cagcgcgccg gtgcaggtgc agttcatcgt ggggcggttc
     3121 gccacaggcg gcacatcgga gattgacggc tacagcgtgg taccggacag cttgtgggat
     3181 aacgaatatt acagcccggt gggaagcgcg gcagtgggtg gcggggacgt tgatctcttc
     3241 ctgtacaacc ccaacgcctc cgcaatcacc atcagtttcg aggacctgac cggatccggc
     3301 agcttcagcg tcccagcatt gagcacccgc tcgtattcgg atccgctggg cgccgggcga
     3361 ttggtcccca acaactcagg cgtgtatctg tcctcgtcgt caattttctg ggggattggt
     3421 tcgggcgacg ccgaatcggc caactgggac tggggctaca gcctggtccc ggtcaacgcg
     3481 ctgcaggacc actatttcct aggctgggcc ccgggaacct cccaggccgt tccgaccagc
     3541 aatgggtctc cggcctatgt cacgccagtc caggacgaca ctacggtgtt cattgactat
     3601 agcccgacca acgggaccgt cgacgctacg ttcactctca atcgtctcca gacccagcgg
     3661 gtcttcgacc ccgacaatga gaacaccggc atgcatatct tcgccaccgg tgacatcgct
     3721 gtcgcctggg gcgaggaccc gaacgtcgcg gccaccggca cgccctatct ggatctgggc
     3781 tattccacgc tgccgttccc gcccgagtgg atggatttgg tcctgggcat tgagaagacg
     3841 gccaatccta cttctctgcc gcaggccgcc ggccagatct cgaccttcac cctggtggtc
     3901 agcaccttca ccttcccggt ggatgacgtc gatgtgttcg ataccctccc gccgggctgg
     3961 gtgtatgtag ccggcagcac gaccatcacc ctgccggaca actcgacgat taccggaaac
     4021 gcggctgacc caacaactat cgtggggcaa gtgctgactt gggacctgaa ccaagacatc
     4081 ccgatcgccg aggccctgac cgttgtattc caggcgcaga ccacggcagg cgtgtcaggc
     4141 ggcctcaacc agaacgatgg agaagcctcc gggacccgcc tgggcggagc gcaggtcttt
     4201 gccccgatcg acagcgcgtt cgtgttcctc tccccgaccg gcctgtcgat cgacaaagac
     4261 accagcacgg ccacggtaga cccgggcacc accgccagct attcgattat cgtcgccaac
//
LOCUS       DATDPQ010000301         6712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2267414, whole genome shotgun sequence.
ACCESSION   DATDPQ010000301 DATDPQ010000000
VERSION     DATDPQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267414"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VI520_06725"
     CDS             complement(<1..465)
                     /locus_tag="VI520_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37611.1"
                     /translation="MQLKGDRMPFAGASRSRPVRVVLLLALIAGGILLTRLVEAGRVK
                     PLLLPTATPTRTAFSFQEEGEAYFSAGNLDKAIQAYQQAVREEPGNAELWARLARIQT
                     YSSALMPTEVERTLRLEQARASADRAVEVDPDSDLAWAIRALVYDWNAGAQEV"
     gene            complement(481..1647)
                     /locus_tag="VI520_06730"
     CDS             complement(481..1647)
                     /locus_tag="VI520_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37612.1"
                     /translation="MILVAGVIYFERTVVPRTPPLFIPTAVPTRSPATFILEAESLFQ
                     AGKLEQAEAAYLQAIEVDPDTVQFYTELARVQVFSGKYDQAVTNASNALLLDPNSAMA
                     HAVLGWALDFQAQAGSSTATSLEALEKLERAIELDPNSPLVRAYYAEVIIDDDVTAFQ
                     LAREQAELAVQLDPNLFESQRALGYVWERTSNYELAFEAYQSALRINPNLALIHIALG
                     NMYFTRGETRLAIDSYVRAMTLAPQDIVPLRLIAQAYARDGEFGKASQYALTALSLQP
                     TNPRLHGDLGRMYYKNGDYEAAVRSLTLAIQGGPNDEGAPVEGLPLDPGDALVIDFYS
                     MYGLALARQSQCGVAIEVAQALVTGVPDSEIAAGNAQEILILCGQIEAPPETES"
     gene            1822..2811
                     /locus_tag="VI520_06735"
     CDS             1822..2811
                     /locus_tag="VI520_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37613.1"
                     /translation="MLPAGHAVSGPTAPSRSIRRALRWIVPFTVLLLALAAAVGYLVG
                     DAERERTQRTEIQRIIQEQYTLGLLDLDHENFEIARQRFEYIIHLDPSFPGAAEQLAE
                     ALLGLREPPPTPASSAATPTPNLAPIEEMLGQAQVMFSAGEWTATIETLLALRAKDPA
                     YRSVEVDGLLYAALRNRGIQRISQDHLLEEGIYDLSQAGSFAPLDEEASNWRGWAQLY
                     LTANSYFGLNWEQAAFYFELVSTVAPGIRGDVSWKYAVSLRMYGLQLAAEGDPCLAEE
                     LMTISLEVLPDEAIAPTATAVHEACQIATAPAPPPDATPTPSETPTVTSTPEG"
     gene            2822..3310
                     /locus_tag="VI520_06740"
     CDS             2822..3310
                     /locus_tag="VI520_06740"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLF37614.1"
                     /translation="MPLWALTLAFWLHMAATVVWIGGLFFQALVLPPALRRLPERGQV
                     LEALARRFQPLAWLSLAVLVGTGLTQMAANPNYTGVLAFGNAWSRAILAKHLAVGLMV
                     AVGIYQTWVVQPALVRMAILEARTPQADHGPLLRRQDRLARLNLALGIAVLAFTAIAR
                     TV"
     gene            3383..3838
                     /locus_tag="VI520_06745"
     CDS             3383..3838
                     /locus_tag="VI520_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37615.1"
                     /translation="MVLHFSLWSGIVGLYLAIAGALGAIIRQAIATGKPRWDRLLAAA
                     YTFLLFGAASAFVLTVTDWPAWSRLGLLAFGLFAAGVAAARPIWVPAPFWRPNFGHRY
                     LAGVMALAALWGINQAMAGSPTAPLMIAVSALAAAAASSGTSLRIPSFE"
     gene            3885..4805
                     /locus_tag="VI520_06750"
     CDS             3885..4805
                     /locus_tag="VI520_06750"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HLF37616.1"
                     /translation="MFELLFLGTSASAPSVHRGLSAQIVSFRDYRFLVDCGEGTQRQI
                     LRAGVGFRRLNRILITHGHLDHILGLAGLLSTFARWEAVDELQIWASPWALERIHDLL
                     FGVVLRGAKPPLKIELLPIESGNLIEDEDFRLQAFPVEHRGPGCFGFVFEQASHRPFL
                     PDKASQLGVPEGPARRELVRGRSVTLSDGRRVEPEQVLGPPRRGTRLVHIGDAASTEG
                     LLEACRDADGLVIEATYLEAQHDLAHEFGHLTARQAAELARTAGVGALYLTHISRRYR
                     EKEVLEEAQPIFPSVSVVRDFDRFQIKRAD"
     gene            complement(5205..5507)
                     /locus_tag="VI520_06755"
     CDS             complement(5205..5507)
                     /locus_tag="VI520_06755"
                     /inference="COORDINATES: protein motif:HMM:NF011016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLF37617.1"
                     /translation="MQTSQVHAVITGQVQGVSFRYYTYHEALKLGLKGWVRNLRDGRV
                     EAIFEGEQESIERVLTWCRLGPPAARVEEVQVEWQPPSGKLEAFQVRPTANLGEPA"
     gene            complement(5540..>6712)
                     /locus_tag="VI520_06760"
     CDS             complement(5540..>6712)
                     /locus_tag="VI520_06760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLF37618.1"
                     /translation="AHDYLSRMGIERGEYYPLLLFTVSGMMLMSLAGDLIVVFVALEL
                     LSLPLYVLTGFARPKPESEESAMKYFLLGAFASGFMVYGIALIFGATGTTNLAGVAAE
                     LSGGAGLLLVGAVLVLVGLGFKVAAVPFHMWTPDVYQGAPSSVVAFMSVGAKAGGFAA
                     LLRVFIAALPDLAASWAPLTIALAALTMTWGNVAAIVQGNIKRMLAYSSIAHAGYILM
                     ALPAASVPAVAPGAVSAALFYLFAYLFSNLGAWGVVLAVERTEGGGLQIDDYAGLSRR
                     RPGLALAMGLFMLSLTGLPPTVGFVGKFFIFRAALDAGQIGLAVVGVLTSVVSAYYYL
                     RVVVVMYMRSGEPETRSEGWLNATIGVAALATFVFGILPGPVLALAENSGILGVLR"
BASE COUNT         1217 a         2204 c         2017 g         1274 t
ORIGIN      
        1 gacctcctgg gctccggcgt tccagtcata caccagtgct cggatcgccc aggccaggtc
       61 gctgtcaggg tccacctcca ccgcccgatc ggccgaggct cgggcctgtt ccagccggag
      121 ggttcgctcc acctcggttg gcatcagcgc gctggaatag gtctggattc gggccagccg
      181 ggcccacaac tctgcattcc ccggctcctc gcgtacagct tgctgatagg cctggatggc
      241 cttatccaag ttccctgcgg agaaatacgc ctccccttcc tcttggaagg agaacgcggt
      301 gcgggtcggg gtggccgtgg gcaacaagag cggcttcact ctgccggctt cgaccagccg
      361 ggtcagcagg attccgcctg cgattagggc cagcagcagc acgacccgca caggacgcga
      421 gcggctcgcg ccggcaaacg gcatccgatc acctttcagt tgcacaaacg ttctccgcgg
      481 ctaggactcg gtctctggcg gggcttcgat ctgaccgcaa aggatcagga tctcttgggc
      541 atttccggcg gcgatctcgc tgtcgggtac tccggtgacc agagcctgcg cgacctcaat
      601 cgccacaccg cactgggatt gtctggccag cgccaagccg tacatcgagt agaaatcgat
      661 gaccagcgcg tcgcctggat cgagcggaag cccttccacc ggggctccct catcattggg
      721 gccgccttga atagccaggg tcagcgagcg gaccgcagcc tcatagtcgc cgttcttgta
      781 gtacatccga ccaaggtcgc catgcagccg cggattggtg ggctgcagac tgagcgcggt
      841 cagcgcatac tggctggcct tgccaaattc cccatcccgg gcataggcct gagcaatcag
      901 ccggagcggc acgatatctt ggggagctag agtcatggcc cgcacatagc tgtcaatcgc
      961 caggcgagtc tcgccgcggg tgaagtacat gttgccaagc gcgatgtgga tcagtgccag
     1021 attcgggttg atccgcagcg cactctggta ggcttcgaaa gcaagctcgt agttgctggt
     1081 ccgctcccat acgtaaccca gcgctcgttg ggattcgaac aggttcggat ccaattgcac
     1141 ggccagttct gcctgttcac gggccagctg aaaggcggtg acgtcgtcgt cgatgatgac
     1201 ttccgcgtag taggcccgaa ccagcggaga attcggatcc agctcgatgg cccgctccag
     1261 cttctcgagg gcctctaagc tggtcgccgt acttgagccg gcctgcgctt ggaaatccag
     1321 cgcccaaccc aggaccgcat gggccatggc cgagttcgga tcgagcaaca aggcattgct
     1381 ggcgttggtc accgcctgat cgtacttccc tgagaagacc tgaacccggg ccagctcggt
     1441 gtagaactgc accgtatcgg ggtcaacctc gatcgcctgt agataggccg cttcggcttg
     1501 ctctagcttc ccagcctgga agaggctttc cgcctccagg atgaacgtgg ccgggctgcg
     1561 ggtaggtacc gcggtcggta tgaacaacgg cggcgtgcgg ggaaccaccg tacgctcaaa
     1621 atagatcacc ccagcgacaa gaatcatcag cgcgatcact cgccagggac tggtgcggcg
     1681 gcgacggcgg gtggcacggg tattccactt actgccgctt agatacatat gggggtattc
     1741 gatgttacca cagccggttt tttcaccgac taggatctgc tcgataaggg gcgcaaaggg
     1801 ggctacagta taatgccgcc cgtgctcccg gcaggacacg ccgtctccgg cccgaccgct
     1861 ccgagccgat ccatccggcg ggccctgcgg tggatcgtcc ccttcacagt cctgctgctg
     1921 gccctggctg ccgccgtggg gtacctggtc ggcgacgccg agcgggagcg gacccaacga
     1981 acggaaatcc agcgcattat ccaggagcaa tacacgctcg gactcttgga tcttgaccac
     2041 gagaactttg agatcgcccg gcagcgattc gaatacatca tccacctcga tccgagtttc
     2101 cccggcgcag ccgagcagct ggccgaagcc ctgcttggcc tgcgcgagcc gccccccacc
     2161 ccggcttcct cggctgccac tcccactccg aatctggctc cgatcgaaga gatgctcggc
     2221 caggcccaag tgatgttttc agccggggaa tggaccgcga ccatcgagac cctgcttgct
     2281 ttgcgggcca aggacccagc ttatcgatcg gtagaggtgg atggattgtt gtacgccgcc
     2341 cttcgcaatc gcgggattca acgcatctcg caagatcatc tcttggagga ggggatttac
     2401 gatctgagtc aggcgggaag ttttgccccg ctcgacgaag aggccagcaa ctggcgcggc
     2461 tgggcccagc tctatctgac cgccaacagc tacttcggac tgaattggga gcaggccgcc
     2521 ttctattttg aactggtgtc cactgtggcc cccgggatcc ggggtgacgt gagctggaag
     2581 tacgccgttt cgctgcgcat gtacggactg caactggcgg cggaagggga tccttgcctg
     2641 gccgaggagc tgatgacgat ttctctggaa gtgttaccgg acgaagccat cgcccccacc
     2701 gcgactgccg tccacgaggc ctgccagatc gcaaccgcac ccgcaccgcc tcccgacgcg
     2761 actccaaccc cgtcagagac gccgaccgtg acctcgactc ccgaggggta aggaagcaca
     2821 agtgccgctc tgggccctca ccttggcctt ctggctgcat atggcggcga ccgtcgtctg
     2881 gatcggggga ttgttcttcc aggcgttggt tttgccaccg gcgctgcgcc ggctgcccga
     2941 gcgcggccag gtcttggaag ccctcgcccg tcgcttccag ccgctggcct ggctcagtct
     3001 ggcggtgctg gtcggtaccg gtctgactca aatggctgcc aatccaaact acaccggggt
     3061 cctcgctttt gggaatgcct ggtcgcgagc gatcttggcg aaacatctgg cggtggggct
     3121 gatggtcgcg gttgggatct accagacctg ggtagttcag ccggcactgg ttcggatggc
     3181 catcctcgaa gcgcgcacgc ctcaggccga ccacggtcca ctgcttcgca gacaggatcg
     3241 cttagcgcgg ctgaacctgg ccctggggat tgccgtcctg gccttcactg cgattgcccg
     3301 cacggtctga tctgaagccc tcctgccctc cgcgatgggc atgcaacttg catcgagccc
     3361 atccagaact tcggaggcag cgatggtctt acacttctca ctgtggtctg ggatcgtcgg
     3421 cttgtatctg gcgatcgctg gggcgctcgg agccatcatt cggcaggcaa tcgcaacggg
     3481 caagccccgc tgggaccggc tcttggccgc cgcgtacacg tttctgctct tcggcgcagc
     3541 ctcggccttc gtcctgaccg tcaccgattg gccggcttgg tcgcggctgg gcctgcttgc
     3601 ctttgggctc ttcgccgcag gggtggccgc ggctcgaccg atctgggtcc ccgccccttt
     3661 ctggcggccg aacttcggcc atcgttattt ggcgggggtg atggcactgg cagctctctg
     3721 ggggatcaac caggccatgg ctggatcccc aaccgctccg ctaatgatcg cagtctctgc
     3781 gctcgctgct gctgcggcgt cttccgggac ctcgctgagg attcccagct tcgagtgagg
     3841 tacaatgacc ggcccgattc tccccaccca gtcctggatc tcgtgtgttc gagcttctat
     3901 tccttgggac ctctgcctcc gctccatccg tgcaccgcgg cctcagcgcg cagatcgtaa
     3961 gttttcgaga ctaccgcttc ctggtcgatt gtggcgaagg cacccaacgg cagattctgc
     4021 gggccggggt cggttttcgc cggctcaatc ggatactgat cacccacggc cacctcgatc
     4081 atattctcgg gctggccggt ctgctctcca cttttgcccg ctgggaggca gtggatgaat
     4141 tgcagatttg ggcgagtccc tgggcgctcg agcggattca cgacctgctg tttggggtag
     4201 tgctgcgcgg cgccaaaccc ccgctcaaga ttgagctgct cccgatcgaa tccggcaacc
     4261 tgatcgagga cgaggacttt cgcttgcagg cattcccggt cgagcatcgt ggcccgggct
     4321 gctttggttt cgtgtttgag caggcctctc accgaccctt cctcccggac aaggcttccc
     4381 agctcggggt cccggaggga ccggcccggc gcgaactggt cagaggccgc agcgtcacct
     4441 tatccgacgg tcggcgggtc gagcccgagc aggtcctggg gccgccccgc cgcggcactc
     4501 ggctggtgca tattggcgac gcggcctcca ctgaaggatt gcttgaagcc tgccgggatg
     4561 ccgatgggtt ggtgattgaa gccacctacc ttgaggccca gcacgatctg gctcacgagt
     4621 tcggtcatct gaccgcccgg caggcagccg agttggccag aacggccggg gtcggggctt
     4681 tgtatctcac tcacatctca cgccgctacc gcgagaaaga ggtgctggaa gaagcccagc
     4741 ccatcttccc cagtgtttcc gtggtgcgcg acttcgaccg gttccaaatc aaacgagcgg
     4801 attgagcccg atgctcacgg tcgaccaagc ggcggccggc ctgctcctgc agttggcatt
     4861 tgaccgactc gggccgggcg ggtagaattg aaatagagac cccaacgatg ccaatctacg
     4921 agtacgtttg cctcgactgc aacacgttat tcgacgccct gcgcccaatg gcgaaggcgg
     4981 atgcgccaat cgtgtgcgag gactgtgaaa gcgagcacac ctcgcgcatg ttatcggtct
     5041 tttttgccca ttcggagggt cgcagcttcc ccgccagcaa tggtgccagc tgcgcctgtg
     5101 ggggcgcctg ttcctgcgcg agccccaatt aatcagcaat ccgtagaatc gaaaagagcg
     5161 ccggcaacca accgccggcg ctcttgattc cccagccgaa atcctcaggc cggctccccc
     5221 aggtttgcgg tcggccgcac ctgaaacgct tccagcttcc cagatggggg ctgccactca
     5281 acctgaacct cttcgacccg ggctgcaggc gggcccaatc tacaccaggt caacaccctt
     5341 tcgatcgatt cctgctcgcc ttcgaagatc gcttctaccc tcccgtctcg caagtttcgc
     5401 acccagccct tcaaccccaa cttaagcgcc tcgtgatagg tgtagtaacg gaaactaacc
     5461 ccctgcactt ggccggtgat caccgcgtgg acctggctgg tctgcatctc tttctcctgc
     5521 atggagctgc cggggccggt cagcgaagta cccccaaaat ccccgagttc tcggccaggg
     5581 cgagtaccgg tcccggcagg atcccgaata cgaaagtcgc cagcgcggcc actcctatcg
     5641 tggcattcag ccagccttca gaccgagtct ccggctcgcc tgagcgcatg tacatcacaa
     5701 ccaccactcg caggtagtag taggcggaga ctacggaggt gagaaccccg accactgcca
     5761 gtccgatctg ccccgcatcc agggctgccc gaaagatgaa gaattttccg acaaacccga
     5821 ccgtcggagg caggccggtg agtgacagca tgaacagtcc catcgccaac gccaaaccag
     5881 ggcgccgtcg agacaatccg gcataatcgt ctatctgtag gccgccgcct tcggttcgtt
     5941 cgaccgccaa caccaccccc caggccccca gattcgaaaa cagataggcg aacagataga
     6001 acagcgccgc gctcaccgca cctggggcca cggccgggac gctggcagct ggcaatgcca
     6061 tcaagatgta gcccgcgtgg gcgatgctcg agtaggctag catccgtttg atattgccct
     6121 ggacgatcgc ggcgacattt ccccaggtca tggtgagggc agccagggcg atcgtcagcg
     6181 gagcccagct ggccgccaga tcgggcaggg ccgcgatgaa gactcgcagc agcgccgcga
     6241 agccaccggc tttggccccc actgacatga aagccacgac cgacgacggg gcaccttgat
     6301 agacatcggg ggtccacatg tgaaagggca ccgccgccac cttgaagccg agccccacca
     6361 ggaccaatac cgcccccacc agcagcaggc cggctccacc gcttagttcg gcggctaccc
     6421 ccgccaggtt cgtagtcccg gtcgccccga agatcagcgc gatcccgtat accatgaagc
     6481 ctgaggcaaa ggcgccgagc aggaagtact tcatggccga ctcttcagac tcgggtttgg
     6541 gccgggcgaa cccggtcagc acgtagagcg gcaaagacag cagctccaat gcgacaaaca
     6601 ccacgatcag gtctcccgcc agactcatga gcatcattcc gctgacggtg aatagcagca
     6661 gcggataata ctccccccgc tcaattccca ttcggcttaa atagtcgtgc gc
//
LOCUS       DATDPQ010000302         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3853751, whole genome shotgun sequence.
ACCESSION   DATDPQ010000302 DATDPQ010000000
VERSION     DATDPQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853751"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VI520_06765"
     CDS             <1..372
                     /locus_tag="VI520_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37619.1"
                     /translation="EVAERIGPVSGFVLRAANEPSLYVAGDTIWCEQVEAALEAHQPS
                     VVVVNAGAAQFTDSGPITMDAGDVLSVARAAPNSQVVAVHFESINHCVLSRAELRARV
                     EREGLAERISIPADGEVLEYA"
     gene            365..1807
                     /locus_tag="VI520_06770"
     CDS             365..1807
                     /locus_tag="VI520_06770"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HLF37620.1"
                     /translation="MHDLLERAIEQAAHRGAQYADARITENQTESLLMRDGIMEVLDS
                     SQDNGFGIRVLVDGGWGFASSHDLRPAEFERVTDQAIRIAKASAQVPGGKVNLGSPVA
                     SRGSYVTPVRIDPFSVPLDQKIAVLAAADAGMARAAGVRVRESNLVFIKEKKWFANSD
                     GARTEQTIIETGGGMVATAVGEGEVQTRSYPTSFGRQQLTAGWEAVEQWDLPGNAERI
                     GNEAAALLTAERCPAGTSDLILSGDQVALQVHESCGHPIELDRVFGTEAAYAGTSFLT
                     TEKLGTFRYGSEPVNLTADSVRPRGLGTFGWDDEGVPAQSTPIVRAGLFIGYLMSREM
                     ATRIGLESNGCMRASGWNRIPLIRMTNVSLEPGTWTLHDLIADTEDGIYMETNRSWSI
                     DDRRYNFQFGTEIGYEIKNGKLGRLYRNPTYTGITPEFWNSCDAVCGELDWRMWGTPN
                     CGKGQPSQIAHTGHGAAPARFRQVRVGIHQ"
     gene            1804..>2732
                     /locus_tag="VI520_06775"
     CDS             1804..>2732
                     /locus_tag="VI520_06775"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HLF37621.1"
                     /translation="MNLAGGAGMIGEDQARRLAAQALELSRPDRAEVALKGTSSKLTR
                     FANNTVHQNVSESDARVCVRIVQGERVGSAITNRLEPEGLAVAVQKARLFASESAADP
                     HLPAIARQAVAAGLDSFDQATAEHSPDARAEAVGELCRRAAGRGLNASGAFRVAAEEM
                     AAANSEGTFAYHAGTTADFQTVVTGDGSSGWAHAAHWKVGELSLPGLGEEAIRKAVEG
                     KGPRSFEPQATAVVLDPYATADLVLMLNLVGMGAQSLIKGQSWMVDRIGKQAMSEQVN
                     IWDDGRDLEGLPLLFDYEGVAKQRVDIVKGGVV"
BASE COUNT          518 a          826 c          912 g          476 t
ORIGIN      
        1 gaggtggccg agcggatcgg accggtctcc ggctttgtgc tccgggccgc caatgaacct
       61 tcgctctatg tggccggcga cacgatctgg tgcgagcagg tcgaggcagc cctcgaagcc
      121 catcagccct cggtggtggt cgtgaatgct ggcgcggcgc agtttaccga cagcgggcca
      181 atcactatgg atgcaggcga tgtcttgtcc gtggcccgcg ctgctccaaa cagccaggtg
      241 gtcgcggtcc acttcgagtc cattaatcac tgtgtcctca gccgagccga attacgtgcg
      301 cgggtcgagc gggaaggtct ggccgagcgg atctcgattc cggctgacgg ggaggttttg
      361 gaatatgcat gatctcttgg agcgagccat cgagcaggcg gctcatcgcg gagcccaata
      421 cgccgacgcc cgaatcacag aaaaccaaac tgagagcctg ctcatgcgtg atggaatcat
      481 ggaagtcctg gattcttcac aggacaatgg gtttgggatc cgagttctgg tcgacggcgg
      541 ctggggattc gcctccagcc atgatctccg gccggccgaa ttcgagcggg tgaccgatca
      601 ggcgatccgg atcgccaaag cttccgccca ggtccccggc gggaaagtaa acctgggttc
      661 gccggtagcc agccggggca gctatgtgac cccggttcgg atcgatccgt ttagcgtacc
      721 gttggaccag aaaatcgcgg tgctggctgc cgcggatgca ggaatggccc gagccgctgg
      781 ggttcgagtc cgggagtcca atctagtctt catcaaagag aagaaatggt ttgcgaacag
      841 cgacggcgcc cggaccgagc agaccatcat cgagacgggt ggtgggatgg tggccaccgc
      901 ggtaggagag ggcgaggtgc agacccgctc ctacccgacc tccttcggcc gccagcaact
      961 gaccgcgggc tgggaggcag ttgagcagtg ggacctgcct ggcaacgccg aacgaatcgg
     1021 aaatgaggcg gctgccctgc tcaccgccga gcgctgcccg gccggaacct cagacctcat
     1081 cctatcggga gaccaggtgg cacttcaggt ccatgaatcc tgcggccatc cgatcgagct
     1141 ggaccgagtc ttcggcaccg aagcggccta cgcggggacc tcctttctaa cgaccgagaa
     1201 gctgggcacc ttccgctatg gctcggagcc agtaaacctc accgccgata gtgtccgtcc
     1261 gcgcgggctg ggtaccttcg gatgggacga cgagggcgtg cccgcccagt ccaccccgat
     1321 tgtccgggcg gggctcttca tcggatacct gatgtcccgc gagatggcca ctcggatcgg
     1381 gctggagagc aatggctgca tgcgggcatc gggttggaac cggatccccc tgatccggat
     1441 gaccaatgtc agcctcgaac caggcacgtg gacgctgcat gacctcattg ccgacactga
     1501 agacggaatc tatatggaga ccaatcggtc gtggtcgatc gatgaccgcc gctacaactt
     1561 ccagttcgga actgagatcg gctatgagat caagaacggc aagctcggcc ggctgtaccg
     1621 caatcccacc tacaccggca tcacccccga gttttggaac agctgcgacg cagtctgtgg
     1681 cgaactggac tggagaatgt ggggaacgcc aaattgtggc aagggccagc cctcccagat
     1741 cgcccacacc ggtcacggcg cggcgccggc ccgcttccgt caagtgcggg ttgggatcca
     1801 tcaatgaact tggccggagg ggcgggaatg attggcgaag accaggctcg gcgacttgcc
     1861 gcacaggcgc tcgagctaag cagaccggat cgggcggaag ttgcccttaa gggcaccagc
     1921 tccaagttga cccggttcgc gaacaacacg gttcatcaga atgtatccga atccgatgcc
     1981 cgggtctgcg ttcgcatcgt gcaaggtgag cgggtcggtt cggcaattac caatcggctt
     2041 gagccagagg ggctggccgt cgcggtccag aaggcccggc tgttcgcctc cgagagcgca
     2101 gctgacccgc atctgccggc gatcgctcgc caggctgtcg cagccgggct cgattcgttc
     2161 gaccaggcca ctgcggagca ctcccccgac gctcgggccg aggccgtagg tgaattgtgc
     2221 cgtcgggcgg ccggccgcgg cctgaatgcc tcgggcgcct tccgggttgc cgccgaggaa
     2281 atggccgcgg cgaactccga aggcactttc gcctaccatg ctgggacgac tgcagacttc
     2341 cagacggtcg tgacgggcga cgggagctcg ggctgggccc acgccgccca ttggaaggtc
     2401 ggcgagctct cgctgcctgg gcttggagag gaggcaatcc gtaaagccgt agaggggaag
     2461 ggaccccgct ccttcgagcc tcaggccacc gcggtcgtgc tcgaccccta cgccaccgcc
     2521 gacctggtct tgatgcttaa cctcgtcggg atgggtgccc agtccctgat caaaggacag
     2581 tcgtggatgg tcgatcggat cgggaagcag gcgatgagcg aacaggtgaa catctgggac
     2641 gatggccggg atcttgaggg cctgccgctt ctgttcgatt acgaaggagt ggcaaagcag
     2701 cgggtggaca tcgtgaaggg tggcgttgtc ca
//
LOCUS       DATDPQ010000303         5473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2514049, whole genome shotgun sequence.
ACCESSION   DATDPQ010000303 DATDPQ010000000
VERSION     DATDPQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514049"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VI520_06780"
     CDS             <1..538
                     /locus_tag="VI520_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HLF37622.1"
                     /translation="SHPELVPRRISLFVLPALNPDGEARSHGRPGRANENGVDLNRNW
                     PSSWQAEWPKDGCWNYLPINGGAAPASEPEVEALLSFVLEKRFDSIISYHSAALGIFP
                     GGQPPPADSVRLAEAVAAVSPYPYPPINSGCLFTGQFADWVADRGIAAIDLELETHRN
                     TDLEINLRVLEVFLNWRR"
     gene            complement(545..1810)
                     /locus_tag="VI520_06785"
     CDS             complement(545..1810)
                     /locus_tag="VI520_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLF37623.1"
                     /translation="MKRSEFMHVLRESCCEPAAADRVRQTWPAWMPRLAFGDAARGDI
                     LVVVFLRGAADALNVIVPHGDPAYYAQRPTLAIARPDDPGAKPADRVVDLDGFFGLHP
                     ALRPLLPAWQQGHLAPIHACGAPDESRSHFKAMELMERGVEDPAGPASGWINRHLASL
                     DTGNPSPLRAVGLGEAAQRSLQGAIPVTALRSISEFHLAGDRRIEQLMQAGLSSLYRG
                     DDPLGRLGRETLRILDTLERLHPDSYRPRETAVYPNSEFGLGLLQIAMLVKAEVGLEV
                     AAVDLGGWDTHFAQGANEGHMAGLLADLGQGLAALHADLADFHSRLSVVVMTEFGRRV
                     QENASLGTDHGHGSLMLLLGGNVVGNKIHGRWPGLEQDQLFGPGDLAVTTDYRDVLSE
                     ICGLRLNNRALSQVFPNFRPRESGFVRRG"
     gene            complement(1839..3284)
                     /locus_tag="VI520_06790"
     CDS             complement(1839..3284)
                     /locus_tag="VI520_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HLF37624.1"
                     /translation="MTLSRREFLRLAGLVAGGVTVSACSPLYRQLSGLPATLAGWPPV
                     SAWDFQVLSRLTYGPTEQDRLDINGLGWKSWIEMQLSPESIEDQAVTWRLRSLESIGS
                     DPDGLADWDKEVLIDELKQAALLRRVYSRRQLYERMVEFWSDHFNISVWKGDCWFLKA
                     IDDREVIRQHALGDFRDLLWASAHSPAMLVYLDNQVNEGAAPNENYARELMELHSLGV
                     DGGYSQSDVMELARCLTGWTVKRHFWQGQFTFNPDLHDPEPKFVLGRRVEPAGQREAE
                     SVLEELALHPSTARHVAIKLLRRFVSETPPARLIEDTAQTFLATHGSIRAVLDRLLLD
                     VLPEWGAKVKRPNDFIAGGLRILAADTDGARPIQDYLRQMGQPAFEWPTPDGPPDRSD
                     YWISNLMPRWRFALALAQNQISGTTLDLDGLATAAGGDPLKRMSELLLGLQLDPELSG
                     GLQQAIGQVDEQTQLQLSLAGLLASPGFQWH"
     gene            complement(3281..3811)
                     /locus_tag="VI520_06795"
     CDS             complement(3281..3811)
                     /locus_tag="VI520_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37625.1"
                     /translation="MADVYAVFGTLIGLGIAYPGMLTTWWLMFPRLVSRASGRLEHTP
                     WRCLAIGGGLALPLGFVIALLLSAPNGPVQFVGATLLFLSLATATLGASGLADLMGRR
                     LTSKVGQSGSLLTGFVRGAVALELAAAFPGIGWFLVIPFGTVAAFGAATFALLGWMPA
                     RKSAPGIEPAFGQALG"
     gene            complement(3904..4383)
                     /locus_tag="VI520_06800"
     CDS             complement(3904..4383)
                     /locus_tag="VI520_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37626.1"
                     /translation="MNDGVAMLIAAPVVFGSLVALFSLLRVLFAVQVGSAKLAAIEMP
                     GRSFALGTVNLLLLSVIVAALGALGGGGGGIFQLLALILVGLLVIALGFGLAGMSVLI
                     GERLLPDSSLARQTAWGSATLVLASLTPFLGWFGLFPYLAMLGLGGFLIGLFRRSPR"
     gene            complement(4380..4937)
                     /locus_tag="VI520_06805"
     CDS             complement(4380..4937)
                     /locus_tag="VI520_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HLF37627.1"
                     /translation="MTDHLDDSLLHAYLDGALAPDERDGLEEHLNSCPACTEDLARAR
                     RLFLAIGGMSEESLGRDLTPAVLQALSRLPRWVPSLAIGELLGALGIGAALILGLGTS
                     EIVDGLSASSERIATQLEVASRQLTSGWAEALERSRSFWSLSEIRLPDLGSAGIWATI
                     AIAALILWFVGNGLVLGQVRRKVWR"
     gene            complement(4930..>5473)
                     /locus_tag="VI520_06810"
     CDS             complement(4930..>5473)
                     /locus_tag="VI520_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF37628.1"
                     /translation="EPADRDLVLRTRRGEVQAFGELVRRYQSAVFNVCYRMLGERMEA
                     EDLAQETFIRAYRRLATYDADRPFGPWVRRVATRVCLNHLDRRTPPSQELDEDRDRPL
                     VAAGNEPENVLAGAQASAGLRRAITQLPPHYRAVIELRHYQEMSYAEIAELLEIPLSD
                     VKSHLYRARKILARSLMESD"
BASE COUNT         1038 a         1808 c         1734 g          893 t
ORIGIN      
        1 cagccatccc gagctggtcc cgcgccggat cagtctgttc gtactgcctg ctttgaatcc
       61 ggatggggaa gcccgctccc acggccggcc cggtcgggcg aacgagaacg gagttgacct
      121 gaatcgaaat tggccgtcca gctggcaggc ggagtggccg aaggatggtt gctggaatta
      181 cctgccgatc aatggaggcg ccgctccggc ttcggagccg gaagtggagg cgctcctgtc
      241 gttcgtcctt gagaagcggt tcgattcgat catcagctat cacagtgcgg cgctggggat
      301 ctttccgggc gggcaacccc cgcctgccga ctcagtccgg ttggccgaag cggtggcggc
      361 ggtctctccc tatccgtatc cgccgatcaa ctcgggctgc ctgttcaccg gccagttcgc
      421 ggactgggtc gccgatcggg ggatcgcggc gattgatctc gagttggaga cccaccggaa
      481 caccgacttg gagatcaatc tgcgggtgct cgaggtcttc ctgaactggc ggcgctgatc
      541 ggagtcatcc gcggcggacg aatccagact cccgcggccg aaaattcgga aagacctgag
      601 agagcgcccg gttgttgagc cgaagtccgc agatctcgct caacacgtcg cggtagtcgg
      661 tcgtcacggc caggtccccg gggccgaaga gctgatcctg ctccagtccg ggccaccggc
      721 catgaatctt gttgccgacc acgttcccac ccagcagcag catcaggctg ccgtgcccgt
      781 ggtccgtgcc gagactggcg ttttcctgca cccgccggcc aaactcggtc atgacaacaa
      841 cgctgagcct ggaatggaag tccgccagat cggcgtgtaa agccgccagg ccttgcccga
      901 gatccgccag caatccggcc atgtgcccct cattggcccc ttgggcaaag tgagtatccc
      961 agcctcccag gtccacggct gccacctcca gtccaacctc cgccttcacc agcatcgcga
     1021 tctgtaagag gccaagcccg aactcgctgt tcgggtagac cgccgtctcg cgcggtcgat
     1081 acgaatccgg atgcagacgt tccaaggtat ccaggatgcg gagggtttcg cgccccagcc
     1141 gacccaatgg gtcgtcgccc cggtagagcg aggatagccc ggcctgcatg agctgttcga
     1201 ttcgtcgatc gcccgccaga tggaattcgg agatcgagcg caaggcggtg accgggattg
     1261 ccccctgcag gctgcgctgg gcagcttcac ccagaccgac cgctcggagc ggcgacgggt
     1321 tgccggtatc caggctggcc aagtgacggt tgatccaccc cgaggccggt ccggccgggt
     1381 cctcgacccc acgctccatc agttccatcg ctttgaaatg gctgcgggat tcgtctgggg
     1441 cgccgcaagc atggatgggt gccaggtggc cctgctgcca ggccggcagc agcggccgca
     1501 gggcgggatg gaggccgaag aatccatcca gatccaccac ccgatcggcc ggctttgctc
     1561 ccgggtcatc cggccgggcg atcgccaggg ttggccgctg agcgtaatat gcgggatcgc
     1621 cgtgcggaac gatgacgttc agcgcatccg ccgcgccgcg caggaagacc acgaccagaa
     1681 tgtccccgcg ggctgcgtcg ccgaaggcga gccgcggcat ccaggccggc caagtttggc
     1741 ggacccgatc ggcagccgcc ggttcacagc acgattcgcg cagcacatgc atgaactcgg
     1801 accgtttcat ggtctcctca cctttcgctg cgaatggcct agtgccactg gaacccgggc
     1861 gacgccaaca gtccggccag tgacagctga agttgggttt gctcgtcaac ttggccgatc
     1921 gcttgctgca agcccccgct cagctcagga tccagctgga gcccgagcag cagctcagac
     1981 atccgcttga gagggtcccc gcccgccgcg gtggcgagtc catccaggtc cagggttgtt
     2041 ccggagatct ggttctgggc gagggcgagt gcgaaacgcc aacgcggcat gagattgctg
     2101 atccagtagt ccgaccggtc cgggggccca tccggcgtcg gccactcgaa ggcaggctgc
     2161 cccatctgcc gcaggtagtc ctggatcggt cgggccccat cagtgtcggc cgctagaatc
     2221 cgcagcccgc ctgcgatgaa atcattcggc cgcttcactt tggcccccca ctccggcagg
     2281 acatcgagca gcaggcgatc gagcactgct cgaatcgacc catgggtggc caggaaggtc
     2341 tgagccgtat cctcgatcaa ccgtgccggc ggggtttcgc ttacaaatcg acgcagtagt
     2401 ttgatggcga cgtggcgggc ggtagaagga tgcagggcaa gctcttcgag gaccgactcg
     2461 gcttctcgtt gcccggccgg ctcaacccgg cgtccgagca cgaatttggg ctccggatcg
     2521 tgcaggtcgg ggttgaaggt gaattggccc tgccagaagt gccgcttgac cgtccacccg
     2581 gtcaggcagc gcgccagctc catcacatcg gattgggagt agcctccgtc cacccccagg
     2641 ctgtgcagtt ccatcagctc acgggcgtag ttctcgttgg gggccgcacc ctcgttgact
     2701 tggttgtcca ggtaaaccag catcgccggg gagtgggccg aggcccaaag caggtcgcgg
     2761 aagtccccca gggcatgctg ccggattact tctcggtcgt cgatggcttt caagaaccag
     2821 caatccccct tccagaccga gatattgaaa tggtcgctcc agaactcgac catccgctcg
     2881 tacagctgcc ggcggctgta gacccggcgg agcagggccg cttgcttcag ctcatcgatc
     2941 aggacttcct tatcccagtc ggccagccca tccgggtcac tgccgatgct ctcaaggctg
     3001 cgcaggcgcc aggtgacagc ctgatcttcg atggactcgg gtgagagctg catctcaatc
     3061 cagctcttcc aaccgaggcc attgatgtcg aggcgatcct gttcagtggg tccgtaggtc
     3121 agccggctca agacttgaaa atcccaggca ctgaccgggg gccagccggc cagggtggct
     3181 ggcagacccg acagctggcg atagagcggc gagcaggccg agaccgtcac gccaccggca
     3241 accaggccgg cgagccgcaa gaattcgcgc cggctgaggg tcatccgagc gcctggccga
     3301 aggcaggctc aatccccggc gcagacttgc gagccggcat ccagcccagc agggcgaatg
     3361 tcgccgcgcc gaaagcagcc acggtcccaa acgggatgac cagaaaccac ccgattcccg
     3421 ggaaggccgc cgccagctcg agggcgactg ccccgcgcac gaaaccggtc agcaacgagc
     3481 cgctctgccc gaccttgctt gtcaatcggc gtcccatgag atcggccagt ccgctggctc
     3541 cgagggtggc cgtcgcgagg gagaggaaca ggagagtggc cccgacgaac tggactgggc
     3601 cgttcggggc agaaagcagc aacgcgatca cgaacccgag cggcagcgcc agaccacccc
     3661 caatcgccag gcagcgccag ggggtgtgtt cgagtcggcc gctggcccgg ctaacgaggc
     3721 gcggaaacat cagccaccaa gtcgtgagca ttccgggata cgcaatccct aatccgatca
     3781 gagttccaaa tacagcgtag acatcggcca tcgtccggct cctttccgcc gacgagtcgt
     3841 cagcggtgct ggttttgccg gtagtacagg tttggttaga ggtttgtcgc ggggagcggt
     3901 cgactagcgc ggcgaccggc ggaagagccc gatgaggaaa ccgccgagtc ccagcatggc
     3961 caggtaggga aacaaaccga accagccgag aaacggagtg agggaggcga gcaccagggt
     4021 ggcggatccc cacgcggtct ggcgagcgag ggaggagtcc ggaagaaggc gttccccaat
     4081 cagcaccgac atccccgcca gcccgaagcc tagggcgatc accagcagcc cgaccaggat
     4141 cagcgcgagc aactggaaga tccctcctcc gcctccgccg agcgcgccga gcgccgccac
     4201 gatgacgctg agaaggagca ggttgactgt ccccagggcg aacgatcggc cgggcatctc
     4261 gatcgcggcc agcttggcgc tgccaacttg aacagcaaac aacacgcgaa ggaggctgaa
     4321 cagcgccacc aatgaaccga aaacgaccgg agctgcgatc agcatggcga cgccgtcgtt
     4381 catcgccaaa ccttgcgccg aacctggccc aagaccaggc cgttccctac gaaccacaaa
     4441 atcagcgccg cgattgcaat cgtcgcccat atcccggcgg agccaaggtc aggtagccga
     4501 atctcgctca aggaccagaa cgatcgggac cgctccagcg cctccgccca acccgaggtg
     4561 agttggcggc tggcaacctc gagctgggtc gcgatccgct cggaggaggc cgacagccca
     4621 tccacgattt cgctcgttcc cagcccaaga atcagcgccg ccccgatccc gagcgcgccg
     4681 agcagctcac caatcgccag cgagggaacc cagcgcggca gccgcgagag tgcctgcaag
     4741 accgcggggg tgaggtcccg ccccaggctc tcctcgctca tgcctccgat cgcaagaaat
     4801 agccgccggg cccgcgccag atcttcggtg caggcggggc aggaattcaa gtgttcttct
     4861 aagccgtccc gttcgtcggg cgcgagtgcg ccgtccaggt aagcatgcag gaggctgtcg
     4921 tccagatgat cagtcacttt ccatcagact ccttgccaaa atcttccgcg cccgataaag
     4981 atggctcttg acatcgctga gcggaatctc cagcagttcc gcgatctcag catagctcat
     5041 ttcctggtag tgtcgcaatt caatcacggc ccggtagtga ggcggcaact gcgtgatcgc
     5101 ccgccgcagg ccggccgagg cctgggcgcc cgccaagaca ttctccggtt cattcccggc
     5161 ggcgaccagc ggccggtcgc gatcttcatc caactcctgg ctgggcggag tccggcgatc
     5221 caggtggttg agacagactc gggtggccac ccgccgaacc caggggccaa atgggcggtc
     5281 tgcatcatag gtcgccagcc tgcggtaggc ccggataaag gtttcttgtg ccaggtcttc
     5341 cgcctccatt cgttcgccga gcattcgata gcaaacattg aaaaccgctg actgataccg
     5401 ccggaccagc tccccgaagg cctggacttc gccgcgacgg gtgcgaagca ccagatcacg
     5461 gtcggctggc tct
//
LOCUS       DATDPQ010000304         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2515087, whole genome shotgun sequence.
ACCESSION   DATDPQ010000304 DATDPQ010000000
VERSION     DATDPQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2515087"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..555
                     /locus_tag="VI520_06815"
     CDS             148..555
                     /locus_tag="VI520_06815"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HLF37629.1"
                     /translation="MEFGNINVLAVVVSALVAMAVGFLWFSPPLFEKMWLAGIGKTRE
                     EVAGQSPARFLIAFVSALLESYILAALLNIVGGPSIGSALQLAFIIWVSFVAATSAAN
                     FAFAGRKLSLWLVENGNHLLVLFVMALILGAWK"
     gene            complement(561..1331)
                     /locus_tag="VI520_06820"
     CDS             complement(561..1331)
                     /locus_tag="VI520_06820"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF37630.1"
                     /translation="MQIDLTDQVAVVTGASRGIGKAIAAGLADAGAKVVLASRKQEGV
                     DQAAAEISAAGGTALAIAAHAGEEQAVEQLVEKSVAAFGRIDIAVANAGTNPHFGPTL
                     QSTTALWDKIMEVNLKGAFLLSRAVAPIMERQGGGRIIFMASIAGIRPAESLGIYCVS
                     KAGLIMLTRVLANELGPAKIRVNAIAPGTIDTRFSRVLVETPDIAERIVSRTPLGYLG
                     QPQDVVGTALFLASNLADYVTGAVITVDGGLSSHGDLA"
     gene            1431..2339
                     /locus_tag="VI520_06825"
     CDS             1431..2339
                     /locus_tag="VI520_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HLF37631.1"
                     /translation="MRQISTYPGYGPFTRANLRELRRAITEEIYAALGLEPDTRLRHV
                     LNPLVWAPVRRFVQIADRFDQAASQLSFPEAARIVLSRFITRLEVDGRESVPTKGPLL
                     VAANHPGAADVLAIAASLPRTDLKVVVSSVPFLERLPNSARHFIYTAHENSHQRMEVV
                     RSSIRHLRQGGSLLIFPRGVVEPDPELLPGAEESLAEWSTSLGIIARAVPQAKVVPAI
                     VSGVISRRSLRNPVTRLRSEPRERQKLAEVLQILQQMMSPLRPAVSTRLSFGRPIRAI
                     GEDARAVTYQVVETARRLLSEHMAAA"
BASE COUNT          447 a          819 c          836 g          528 t
ORIGIN      
        1 tctggcggtt cgatcgcctt ggtacggacg accaacggcg cccgcacctg gtcgctgctc
       61 gaaccggtca tcggggagta aggcgcgacc gcgcccctac ttgagggttc tgcaagacga
      121 taacggcgaa tcaaggagga atagatcatg gaattcggaa acatcaatgt ccttgcagtg
      181 gtcgtcagtg ccttggtggc tatggcggtg ggatttctct ggttctcacc accgctgttc
      241 gagaagatgt ggctagcggg gatcggcaag acccgggagg aagtggccgg ccagtcgccg
      301 gcccgtttct tgatcgcttt cgtttccgcg ctgctggagt cctatatctt ggcggctctg
      361 ttgaacatcg tcggcggtcc gtcgatcggc tccgccctcc aactggcgtt catcatctgg
      421 gtgagcttcg tggcggctac cagtgcggcc aacttcgcct tcgctggccg gaagcttagc
      481 ttgtggctgg tggagaacgg caaccacctg ctggtgctgt ttgtcatggc gctgatcttg
      541 ggggcctgga agtagctcaa ctacgccagg tcgccatgcg agctaaggcc tccgtccacg
      601 gtgatcaccg ccccggtcac ataatcggct aagttcgagg cgaggaacag ggccgtcccc
      661 acaacgtcct gcggctggcc gaggtaaccg agcggagtgc gtgagacgat ccgctcggcg
      721 atgtccggtg tctcgaccag gacccggctg aaacgggtgt cgatcgtgcc cggggcgatg
      781 gcgttgaccc ggatcttggc cggcccgagt tcgttggcca gcacccgggt cagcatgatc
      841 agcccggcct tggacacaca gtaaatcccg agactttcgg caggacgaat cccggcgatc
      901 gaggccatga agatgatgcg tccgccgccc tggcgctcca tgatcggggc caccgcccga
      961 ctcagcagga aggcgccttt caagttaacc tccatgatct tgtcccacaa ggcggttgtc
     1021 gactgcaggg tcggcccaaa gtgcggattg gttcccgcgt tagctactgc aatatcgatc
     1081 cgcccgaacg ccgcgacgct cttttcaacc agctgctcca ccgcctgttc ctcaccggca
     1141 tgtgcagcaa tcgccagagc ggtccctccg gcggccgaga tctcggcggc cgcctgatcg
     1201 accccctcct gtttgcggct ggcgagcacg accttcgcgc cggcatccgc caggccggcc
     1261 gcgatcgctt ttccgatgcc gcgcgaagcg ccggtaacca cagccacttg atcagtcagg
     1321 tcgatctgca tggttcctcc gtcctgaagg ggggtattct gcagccgggc ggttgatcgg
     1381 tcaatccgcg acgggccagg ctcggactat aatggagatg gagtgccgaa atgaggcaaa
     1441 tctctaccta tccaggatac ggccccttta cccgcgccaa cctgcgcgag ctgaggcggg
     1501 cgattactga agagatctat gccgccttgg ggcttgagcc ggacacccgc ctgcggcatg
     1561 tgttgaatcc gttggtctgg gcgcccgtcc gccgctttgt tcagatcgca gaccgcttcg
     1621 accaggccgc gagccagctc agcttcccgg aggcagcccg gatcgtgctt tcccgattca
     1681 tcacccggct cgaggtcgac ggccgtgaat ccgttccgac caagggaccc ctgctggtgg
     1741 cagccaatca cccaggggcg gccgatgtct tggcaatcgc agctagcctg ccgaggaccg
     1801 atctcaaggt ggtcgttagt tcggttccgt tcttggagag attgccgaac tccgcccgtc
     1861 acttcattta cacggcgcac gagaattccc atcaacggat ggaggtggtt cgctcttcca
     1921 tccggcactt gcggcaaggc ggttccttat tgatttttcc gcggggggtg gttgagccgg
     1981 atcccgaatt gctgcccggt gcggaagagt cgctggcaga gtggtcgaca agtctgggga
     2041 tcatcgccag ggcggtgccg caggcgaagg tggtgcccgc gattgtgagc ggggtgatct
     2101 cgcggcgaag cctgcgcaat ccggtcaccc ggctgcgcag cgaaccgcgg gagcggcaga
     2161 aactggccga ggtcctgcag atccttcagc agatgatgtc accgctccgg ccggcagttt
     2221 ccacccggtt gagctttggc cggccgattc gggcaatcgg cgaagacgcc cgggcagtca
     2281 cctatcaggt ggttgagacg gccaggcgac tgctcagcga gcatatggct gccgcctgag
     2341 ccgattggat cgggccggcc gccctggcag cactaaactc ccagcccctc caccctcaag
     2401 ctcgagccac gctcgaatct catcccggcc aatgttctaa gcgaggcaca tttcgtgacg
     2461 cttgagatgg gcctggtgct ggccatcctg atcggcgcgc tgatcttgtt ggtcagcgag
     2521 aaggtaagga tggacgtggt ggcgttgttg gtgctgggct ccctcgcgtt gaccaaactc
     2581 gtcgatccgg tccaggccct ttccggcttc agcagcccgg ccgtagttac
//
LOCUS       DATDPQ010000305         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3856941, whole genome shotgun sequence.
ACCESSION   DATDPQ010000305 DATDPQ010000000
VERSION     DATDPQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3856941"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1048)
                     /locus_tag="VI520_06830"
     CDS             complement(170..1048)
                     /locus_tag="VI520_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37632.1"
                     /translation="MMQLDRKQLRDEIHRLYRAEHEALGELGTLDLLDKGRAWDLSAT
                     LGAAGVVVFPHAGVADCGHQIAAAVHACLDCGADRVVVVSVLHAFTDEMEAARIRVAK
                     GGDPSQEEFWGIQGPGITGRAEWLNDHALYSFRHFWKAETRRRGTRGPEVIERYPYLA
                     GGKPDRLPGIDELARLAEGAALVSTVDPFHHGIGYGDAPEESLHPLEGGLERAGQVIS
                     EGMRLLAEGDYWGYNQHCVSAKSDGRDAGQVFRYLRGPMTGRILDLSFTDATDLYASP
                     SPTWVAGALFEWNLSR"
     gene            complement(1082..1155)
                     /locus_tag="VI520_06835"
     tRNA            complement(1082..1155)
                     /locus_tag="VI520_06835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Val,seq:cac)
     gene            1279..>2701
                     /locus_tag="VI520_06840"
     CDS             1279..>2701
                     /locus_tag="VI520_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HLF37633.1"
                     /translation="MNQLSLRHRDELRVIAERAMMQRGLLPDFSPAVMAEADALVSAA
                     VETDPAIRDLGGLLWCSIDNEDSRDLDQLTAAEPRLGGLATILVAVSDVDAMVKLGSA
                     INGHARKNTTSVYTAAGVFPMLPEKLSTDLTSLGEGQVRLALVIELGIDADGTLAEAE
                     VYRAVVRNQAKLAYKGVAAWLDGTAPAPPRLAAVPGLYEQLYLQDRAAQALKGLRHQH
                     GALSLETLETRPVFEDDVLADLRPEEKNRAKQLIEDFMIAANGMTARYLDRKGFPTLR
                     RVLRSPDRWDRIVELAARLGGRLGPAPSAPALEAFLAERRQADPVLFPDLSLSIVKLL
                     GSGEYVVELPGQQADGHFGLAVKDYSHSTAPNRRFPDLITQRLLKAALAGRPPPFSYD
                     ELSELARHCTEQEDNAAKVERQVGKSAAALLLESRIGERFEAIVTGASKKGTWVRIFQ
                     PPVEGKVVRGFESLDVGDRVRVEL"
BASE COUNT          472 a          860 c          881 g          488 t
ORIGIN      
        1 cttgccgacc tgctgcatcg ggcgggggac gaggagcagg ccaaggaaca tctgcggcag
       61 tccgccgagc tgttcgccga ggtggggatc gaaaaagatc agtttgaacc ggaaatctgg
      121 aaactggtcg agtggtaggc aggtcgtata gaggagaagg gtcggtgagc taccgactta
      181 ggttccactc gaaaagtgcg ccggccaccc aggtggggga aggagaggca tacaagtcgg
      241 ttgcgtcggt aaagctcagg tcgaggatcc ggccggtcat gggcccgcgc aaatagcgga
      301 acacttggcc ggcgtcccgc ccgtcactct tggccgacac acagtgctgg ttgtatcccc
      361 agtagtctcc ttcagccaac agccgcattc cttcgctgat cacctgaccg gcccgttcga
      421 gtccgccctc aagcgggtgc agcgattctt caggcgcgtc gccgtatccg atcccatgat
      481 ggaacggatc aaccgttgag accagcgcag caccttccgc tagcctggcg agctcatcga
      541 tcccgggcag gcggtcgggt tttccgccgg ccaggtaggg atagcgctcg atcacctcgg
      601 gtccccgagt cccgcgtcgc cgggtttcgg ctttccagaa atggcgaaag ctgtacaaag
      661 cgtgatcgtt gagccactca gcgcggcccg tgatcccggg gccctgaatg ccccagaatt
      721 cctcttggct cgggtcgccg cccttcgcca cccggatgcg agccgcctcc atctcatccg
      781 tgaaggcatg cagcacactg accactacca cccggtcggc cccgcagtcg aggcaggcat
      841 gcaccgccgc cgcgatttgg tgcccgcagt cggcaacccc ggcgtgcgga aacaccacta
      901 cgcctgcggc cccaagcgtt gccgaaaggt cccaggcccg gcccttgtcg agcaggtcca
      961 gggtacccaa ttcgcccagc gcctcatgct ccgcccgata gaggcggtgg atctcgtcgc
     1021 ggagttgctt tcgatcgagt tgcatcatcg gggagagcct cgagggaggt gaggcttcag
     1081 gtgggcgcgg aggggctcga acccccgacc tcacggatgt gaaccgtgcg ctctaaccag
     1141 ctgagctacg cgcccgagct gcttgtcatt ctagcatgcg ccccggacgc tcacaaggaa
     1201 tcgagcttgg cattggcctc cgcccggctt cggtctatga ttggcccgtc cgctccgccg
     1261 ccgtcacaac ctcgtgcgat gaatcaactt agcctccgtc accgcgacga actcagggtc
     1321 atcgcagaac gtgcgatgat gcagcgcggg ttgctgcccg acttctctcc tgcggtcatg
     1381 gcagaggcgg acgcccttgt ctcggccgcg gtcgagacgg atcccgcgat tcgcgacttg
     1441 ggtggtctgc tctggtgctc gatcgacaac gaagacagcc gcgatctgga tcagctcacc
     1501 gccgcggaac cgaggttggg cggtttggcg accatcctgg ttgccgtctc cgatgtcgac
     1561 gccatggtca agctgggctc cgccatcaac ggccatgctc gaaagaacac gacctccgtg
     1621 tatacggctg cgggagtctt cccgatgctt cccgagaagc tctcgacgga tctgacctcc
     1681 ctgggtgagg gccaggtgcg cctggccctg gtcatcgagc ttgggatcga tgccgacggg
     1741 accctcgccg aggccgaggt ctatcgggcg gtcgtgcgga atcaggcgaa gcttgcctac
     1801 aagggcgttg ccgcgtggct ggacggcacg gctcccgcac cgcccaggct ggccgcggtc
     1861 ccagggctct atgagcagct ctatctccag gaccgggcag cccaggcgct gaagggcctg
     1921 cggcaccagc atggggcgct cagcctggag acgctcgaaa cgcggcccgt gttcgaggat
     1981 gatgttcttg ccgacctgcg gcctgaggag aagaatcggg ccaaacagct gatcgaggac
     2041 ttcatgatcg cggccaacgg gatgacggcc cgctatctcg atcggaaagg gtttccaacc
     2101 ttacgcagag ttttgcgctc gcctgatcgg tgggatcgga ttgtcgaact ggccgcccgc
     2161 cttggtgggc gcctgggacc ggcgccgagc gcacccgccc tagaagcgtt cctcgccgag
     2221 cgccgccagg cagatccggt gctgtttccc gacctctccc tctctatagt caagctgctc
     2281 ggttcgggag agtacgtggt cgagctccct gggcagcaag cggacggaca cttcgggctg
     2341 gccgtcaaag attattcgca ttcgactgcg cccaatcgtc gctttccgga tctcatcacc
     2401 cagcggctgc tcaaggccgc gctggcgggc cggccgccgc cgttcagcta cgacgagctg
     2461 agcgagttgg cccgccactg caccgagcag gaagacaatg cggcaaaggt ggaacgacag
     2521 gtggggaaat cagcggccgc attgcttctg gaatctcgga tcggcgagcg tttcgaggcg
     2581 attgtgaccg gcgcctcgaa gaagggcacc tgggtgcgta tctttcagcc cccggtggag
     2641 ggaaaggtcg tgcgcggttt cgagagcctt gatgtcgggg atcgggtgcg ggtcgagctc
     2701 g
//
LOCUS       DATDPQ010000306         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3558224, whole genome shotgun sequence.
ACCESSION   DATDPQ010000306 DATDPQ010000000
VERSION     DATDPQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558224"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VI520_06845"
     CDS             <1..1403
                     /locus_tag="VI520_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLF37634.1"
                     /translation="RTHFVLDGNHRVSVARELGATHIQAYVREVETKVSLSPDVQPDE
                     LIIKAEQAAFLERTRLDEIRPAADFTVTSAGRYDVLEGQIKAHRHSLQLQRPGGVSDA
                     EVVGSWFDRIYLPAVHSIRQISLLREFPGRTEADLYVWITQHQADLERSLGWEIRPES
                     AAADLAARAGLRFRRIIGRIVEKAVGTLTLAPWVPAPAPAQWPEGILATHERAGFTLN
                     ILLPVGGARHDWSAFDQAVLVAQREGARLLGLYVMPSGTKPQRDSAREIQLEFERRAQ
                     AAGVPYGFAIEVGEIAAQIVDRARWVDLVILKLSYPPGPRPLARLRSGFHTIIQNSPR
                     PVLTVPRGAGPLDRALLAFDGSSKSEEALYLATYLAGRWKIPLVVLTVQEGDRDSEAT
                     LERAAEYARDHEVNPVTELRAGPVDEAILATARQHECNLILMGGYGLSPLLEFALGSA
                     VDKVLRKAEVPVLICR"
     gene            complement(1411..1965)
                     /locus_tag="VI520_06850"
     CDS             complement(1411..1965)
                     /locus_tag="VI520_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLF37635.1"
                     /translation="MEHHVRSLAPMTLDNEKLLDPTGWQILLELESNARLSFAELGRR
                     VGLSLPAVAERVRRMEDAGIITGYQVLLDPTKIGRPISAFVRIRLHDHITGERLEQLL
                     ADLPEVLECHNLTGDDCAIVKVAVPSIPDLEQVLQQLKALGQTTTSIVLSSFAHKGLA
                     HAGDRSAANGQLSANGEFVRELVR"
     gene            2098..>2463
                     /locus_tag="VI520_06855"
     CDS             2098..>2463
                     /locus_tag="VI520_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLF37636.1"
                     /translation="MKSRAWLAFGALSVIWGSSFLWIKIAVQELGPFRLVALRLLFGV
                     IGLLIAGRLTKQPLRLSRKQLPAYLVLALFNTALPFTLISWGETRIDSGMASILNGTV
                     PLFTLVIAHFWLQDERITLP"
BASE COUNT          452 a          729 c          776 g          506 t
ORIGIN      
        1 gacggaccca tttcgttcta gatggaaacc accgggtctc tgtcgcccgc gaactcgggg
       61 cgacccacat tcaggcctat gttagagaag tggagaccaa ggtctccctg agtccagacg
      121 tgcagccgga cgagctgatc atcaaagccg agcaagccgc cttccttgaa cggacccgct
      181 tggacgaaat tcgaccggct gcggatttca ccgtcacctc tgccggtcgc tacgacgtgc
      241 ttgaagggca aatcaaagcc caccgtcaca gcctgcaact gcagcgcccg ggaggagtaa
      301 gcgatgcaga agtagtcggc agttggtttg accgaatcta ccttccggcc gtgcattcca
      361 ttcgacagat cagcctgctg cgcgagttcc cgggccgcac cgaggctgac ctttatgtct
      421 ggattacgca gcatcaggcc gacctcgagc gctccctcgg gtgggagatt cggccggaat
      481 ctgctgcggc cgatctggcg gcccgggccg ggctccgctt ccggcgaatc atcggccgga
      541 ttgtggagaa agcagtcggc acgctgacgc ttgctccctg ggtgccggct cccgctccgg
      601 ctcaatggcc ggagggtatc ttggccactc acgagcgggc ggggttcacc ctcaacatct
      661 tgcttccggt cggcggggcg cggcacgact ggagcgcctt cgatcaggct gtgctggttg
      721 cccaacggga aggcgctcga ttgttggggc tgtatgtgat gccgtcgggc actaagccgc
      781 agcgcgattc ggcccgggaa attcagctcg agtttgagcg gcgagcccag gcggccggag
      841 ttccgtatgg ttttgcgatc gaggtggggg aaattgcggc ccaaatcgtc gatcgcgccc
      901 gctgggtcga tttggtgatc ttgaaactgt cctatccgcc tggcccgcgg ccactcgccc
      961 ggctgaggtc tgggttccac acgatcattc aaaactcacc gcgcccagtg ttgacggtcc
     1021 cgcggggagc cggtccgctc gaccgggccc tcctggcatt cgacgggagc tcgaagtccg
     1081 aagaggccct gtaccttgcg acctacctgg ccggccgctg gaagattccg ctggtcgttc
     1141 taactgtgca ggaaggggat cgggacagcg aggccactct ggagcgggcc gcagagtacg
     1201 cgcgcgacca cgaagtaaac ccggtcaccg agctgagagc aggcccagtc gacgaggcga
     1261 tcctggccac ggcgcggcag cacgagtgca acctgatcct catgggaggg tacggcctga
     1321 gcccgctgtt ggaattcgca ttgggaagcg cggtggataa agtgctacgg aaagcggaag
     1381 tgccggtgct gatctgccgc tagagcggga ttagcgaacg agctcccgga cgaactcacc
     1441 attcgcggac agctggccgt tcgctgcaga tcgatcgcca gcgtgggcca gtcctttgtg
     1501 ggcaaacgaa gagagcacga tcgaggtcgt ggtctgaccg agggccttca attgttggag
     1561 gacctgttcg aggtctggaa tgctcggaac ggcgactttc acgatggcgc agtcgtcccc
     1621 cgtcaggttg tggcactcca gcacctccgg cagatctgcc agaagctgtt cgagccgctc
     1681 cccggtgatg tggtcgtgca atcggatccg gacgaaggcg ctgatcggtc gcccgatctt
     1741 ggttgggtct aggaggacct ggtagccggt gatgatgccg gcatcttcca tccttcttac
     1801 ccgctccgcg accgcgggca gtgacaggcc gacccgccgc ccgagctcag caaatgacag
     1861 ccgggcgttc gactccaatt cgagcaggat ttgccatccc gtgggatcta gcaacttctc
     1921 attatctaag gtcataggtg ccagtgatct tacatgatgt tccacttcta gtcaagtacc
     1981 ttaataaacc tatggatttc gctgaattac ctgcatacaa tgactccgta tttgcccgat
     2041 caggattgtt atagtcgcgg cgccggctct gctctaccca tggaggtcgg gaaggctatg
     2101 aaatcaagag cgtggctcgc gttcggagct ttgagtgtga tctgggggtc gtctttcctg
     2161 tggatcaaga tcgcggtcca ggaacttggg ccgttccgat tggtggcttt gcgattgctg
     2221 tttggagtga tcgggttgtt gatcgcgggc cggctgacaa aacagccgct gcggttgagc
     2281 cgcaagcagc tacccgccta cctggtcctg gctctgttta acacggccct gccattcacc
     2341 ctgatctcct ggggggagac tcggatcgac tcgggaatgg cctcgatcct caatggaacc
     2401 gttccattat ttaccctggt catcgcccat ttctggctgc aggacgagcg aatcaccctg
     2461 cca
//
LOCUS       DATDPQ010000307         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2272742, whole genome shotgun sequence.
ACCESSION   DATDPQ010000307 DATDPQ010000000
VERSION     DATDPQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272742"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VI520_06860"
     CDS             <1..573
                     /locus_tag="VI520_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLF37637.1"
                     /translation="QQELLDKIQASVARMTKLVDDLLDLGRIESGIGLSLEAVEISTV
                     LQEVLTNFRPLALNKQVALEVQLGSRLAPVQADPILLRQAIGNLVDNAIKYSQPGTRV
                     LVRADQIEGRQIIQVQDAGVGIASADLPRLFEKFFRVGEGKGAGLGLAIVKSIVEQHQ
                     GRVSVESRLGRGSTFTIAVPMRAERVRAAA"
     gene            801..>3199
                     /locus_tag="VI520_06865"
     CDS             801..>3199
                     /locus_tag="VI520_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLF37638.1"
                     /translation="MEAKGFRALRISLASPETILSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTKDWQCYCGKYKNIRFKGITCEKCGVEVTRASVRRERMGHIGLAAPVAH
                     VWYTRRVPSYLGLLLDISRRNLDRVLYFAQYIVTHVDEEARRRAIKRIEDEIVALDRA
                     QQEAMSARLGEIKSRRKEELEELKARQKQLKAEYEEQVAQLFDPLIREGQRLERELRE
                     KIGKSVRAPIHFKAVKKSIVDKGETVSKEHVSQVQKLVKQELEEIEAGAKKKLDRQLE
                     KLKSQLEQIKDESEQEAEQAKGAPQAESLAQAEALLKQREELETIEQMMFLGETRYRE
                     LKSQWGQVFRADMGAEAFFEILQRLDLAKLAEELWVEVRTSKSKQRKKKATKRLKVVE
                     AFRRSGNRPEWMVLTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLV
                     ELGAPDVIVRNEKRMLQEAVDSLIDNAQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVIGPHLKLHQCGLPKAMALELYRPFVISKLVMYNYVANVKGAKR
                     IIERERPEVWEVLEEVIKDRPVLLNRAPTLHRLGIQAFEPVLVEGKAIQLHPLVCAAF
                     NADFDGDQMSVHVPLSKRAVIEARELMLSSRNLLKPADGEPIVGPSKDMVLGVYYLTM
                     ALDGRGQRGAGRAFSSLDEVEMAYALGQVEVHAPIRLLTTTWYDDQGERQAQAEQKLI
                     ETTVGRVLFNDVLPEELRFVNRVLDKEAMQELVGDVYDLLREEGTPAIVDAIKDIGFA
                     YA"
BASE COUNT          686 a          874 c         1054 g          585 t
ORIGIN      
        1 cagcaggagc tgcttgacaa gattcaagcc agcgttgccc ggatgaccaa actggtcgac
       61 gacttgctcg acctcggtcg aattgagagc gggattggcc ttagtttgga agccgtagaa
      121 atctcaaccg tcctccagga ggtcttaacc aatttccgtc cgctggcgct caataaacag
      181 gtggccctcg aagtgcagct cggttcgagg ctggctccgg tgcaggccga tccgatcttg
      241 ctgcgccagg ccatcggcaa tctggtggac aatgcaatca agtactctca acccggaact
      301 cgggtgctgg tgcgggcgga ccaaatcgag ggccgccaga tcattcaggt gcaagatgca
      361 ggtgtcggca tcgcctcggc cgacctgccg cgcctgttcg agaagttctt ccgggtgggg
      421 gaagggaaag gcgccgggct ggggctcgct atcgtcaaat cgatcgtcga acagcaccag
      481 gggcgggtca gcgtggaaag ccgcctggga cgggggagca ccttcaccat cgccgtaccg
      541 atgcgggcag aacgggtccg agcggctgcc tgagcgccgc cgcagccctc gattctgccg
      601 tgtatgggac ttgacaaatc acttccggcc cgtataatac agtttcgcat gccgagtatt
      661 cgccccaggg attgggcgta ggaacgcttg aggtcggctg aactcaagcg ttctttttgc
      721 atgtatctga tctagcacgt ttgttccatt tgaaccgaca aatcgcaagt agtttcacca
      781 gagaaggcgg aggggaccgc gtggaagcga aaggttttcg agccctgcgg attagtttgg
      841 cgtctcccga gaccattctg agctggtcct acggagaggt gttgaagccg gagacgatca
      901 actatcgccg gctgcggccg gagaaggatg gattgttctg cgaggccatc tttggtccga
      961 ccaaggattg gcagtgctac tgcgggaaat acaagaacat ccgattcaag ggcatcacct
     1021 gcgagaaatg cggggtcgag gtaacccggg cctcggtccg gcgggagcgg atggggcata
     1081 tcgggttggc cgccccggtt gcccatgtct ggtacacccg tcgggtgccg tcctacctcg
     1141 gtttgctgct cgacatttcg cgccggaacc tggaccgagt gctctatttc gcacagtaca
     1201 tcgtcactca cgtcgacgag gaggctcgcc ggcgggccat caagcggatt gaagacgaga
     1261 tcgttgcgct ggatcgggcc cagcaagaag ccatgagcgc ccgactcggt gaaatcaaat
     1321 ccagacgcaa agaagaactg gaggagctca aggctcgcca gaaacaattg aaggccgaat
     1381 acgaagagca ggtcgcccag ctgttcgatc cgctgatccg cgaagggcaa cggttggagc
     1441 gcgagctgcg ggagaagatc ggaaagtccg ttcgggcccc gattcatttc aaggcggtca
     1501 agaaatcgat cgtggacaag ggcgagacgg tctccaagga gcatgtcagt caggtccaga
     1561 agctggtcaa gcaggaactc gaggagatcg aggcgggcgc caagaagaag ctagatcggc
     1621 agcttgagaa gctcaagtcc cagctcgaac aaatcaagga cgaatcggag caagaggccg
     1681 aacaggcaaa gggcgcaccc caggcggaga gcctggctca ggccgaggcg ttgctgaaac
     1741 agcgcgaaga gcttgagacg atcgagcaga tgatgttcct gggggagacc cgctaccggg
     1801 agctcaaatc ccagtggggg caggtcttcc gagccgacat gggcgcagag gcgttcttcg
     1861 agattctcca gcgactggac ttggccaagc tggccgagga actctgggtg gaggtccgga
     1921 cttccaagag caagcagcgc aagaagaagg ccaccaagcg gctcaaggtg gttgaagcct
     1981 ttcgccgctc cggcaatcgc ccggagtgga tggtcctgac ggtgctgccg gtgatcccgc
     2041 cagacctgcg gccgatggtg caactggatg gaggtcggtt tgcgacctcc gacctgaacg
     2101 atctgtaccg ccgggtcatc aatcgcaaca atcggctcaa gcggctggtt gaactcggtg
     2161 cgccggatgt gatcgtccgg aacgaaaagc gaatgctgca ggaggccgtc gattccctga
     2221 ttgataacgc gcagcggggt aaggcgctat cgcggcgcgg ccggcgggaa ctgaagtcgt
     2281 tgagcgacat gttgaagggc aagaaggggc gcttccgacg caatttgctt ggcaagcggg
     2341 tcgactactc ggggcgttcg gtgattgtga tcggcccgca cttgaaacta catcaatgcg
     2401 ggctgcccaa agcaatggcg ctcgagctgt accggccttt cgtgatctcc aagctggtca
     2461 tgtacaacta cgtggcgaat gtgaagggcg ccaagcggat catcgaacgc gagcgccccg
     2521 aggtctggga ggtgttggaa gaggtcatca aggaccggcc ggtgctgctc aatcgagctc
     2581 cgaccctgca tcggcttggg atccaggctt tcgagcccgt gctggtggaa ggcaaggcga
     2641 ttcagctcca tcccctggtc tgcgccgcat tcaacgccga cttcgacgga gaccagatgt
     2701 cggtccacgt gccgctctcc aagcgggccg taatcgaggc ccgggaattg atgctttcca
     2761 gccggaactt gctcaagccc gctgatggcg aaccgatcgt tggaccctcg aaggacatgg
     2821 tgctcggggt gtactacctg accatggccc tcgatggacg cggccagcgg ggtgccggac
     2881 gcgcgttttc gagcttggat gaagttgaaa tggcctatgc gttgggtcag gtcgaggtcc
     2941 acgccccgat tcggttgctc acaacgacct ggtacgacga ccagggggag cggcaggccc
     3001 aggctgagca gaaattgatc gaaaccaccg tcggtcgagt gctcttcaac gacgtgctcc
     3061 cggaggagct gcgcttcgtc aaccgggttt tggacaagga agcgatgcaa gaactggtgg
     3121 gcgacgtcta cgacctgctg cgcgaggaag ggactccggc gattgtcgac gcgatcaaag
     3181 acatagggtt cgcttacgc
//
LOCUS       DATDPQ010000308         5370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3558374, whole genome shotgun sequence.
ACCESSION   DATDPQ010000308 DATDPQ010000000
VERSION     DATDPQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558374"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /gene="ssrS"
                     /locus_tag="VI520_06870"
     ncRNA           <1..173
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VI520_06870"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            314..1324
                     /gene="recA"
                     /locus_tag="VI520_06875"
     CDS             314..1324
                     /gene="recA"
                     /locus_tag="VI520_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLF37639.1"
                     /translation="MPSQTSDQRERALESAVSAIKKQFGDGAVMRMGEATHLAVESIP
                     TGSLALDQALGVGGIPRGRVSEIYGPEASGKTTLCQHIVAESQRLGGLVAYIDMEHAL
                     DPAYAAVCGVDVDNLYIAQPDTGEQALEITETLVRSGAIAVVVVDSVAALVPRAEIEG
                     DMGEPTMGLQARLMSQALRKLSGAIKQTNTAVVFTNQLRQRIGVMFGNPETTSGGMAL
                     KFYASVRIDLRRIESIKVGQEVVGNRVRARVVKNKVAAPFRVAEFEILFNEGISKVGG
                     ILDLAVENGLVDKKGSFYLHGESRLGQGRENAKQYLRENPSLAAELEKGVRAAFAATA
                     LA"
     gene            1673..2335
                     /locus_tag="VI520_06880"
     CDS             1673..2335
                     /locus_tag="VI520_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HLF37640.1"
                     /translation="MTRVTAFVFMQGMITALKPTRRGRVRVYLDGRPGLELAKELAAG
                     LELGRQLGAQQVSELQVRDREESAYRQAVRLLARRPYTEWELRRRFDRTGLTEQEQDA
                     VMGSLRQATLVSDAEFAAAWLENRMAFRPRGAWALREELRARGVPAEVIESTLSGFDE
                     QQAALQAARQGLRKYQHLSPELFRQRLSAYLHRRGFQQSTIGRLVEQLAVDRNAESED
                     ST"
     gene            2332..3867
                     /gene="rny"
                     /locus_tag="VI520_06885"
     CDS             2332..3867
                     /gene="rny"
                     /locus_tag="VI520_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HLF37641.1"
                     /translation="MNAPWLLPVVGSLAALAIGLTLGYLFRQLQLRRELKGRQAEAER
                     ILEEAKGKARELELGGRDEALKLRDQAEAEVSRRRSDLAKEEERLQKRRVDLDGRLDN
                     LEKRDQTINKRQSALDKRSNELETKYAETVAELERVSGMTRDDAKGQLLKEVEREARA
                     DMVKVIHQIEEEARVEGDRRARGLIADAIQRVASDHVAEITTSNVPLPSDDMKGRIIG
                     RNGRNIRAFEQAAGVDVIVDDTPEAVTISSFDSVRREVARRSMERLVLDGRIHPANIE
                     KIVIQTRDEVEHLIAEEGERAAYDAGVPGLRQEITKKLGRLKFRTSYGQNQLAHAIEV
                     AQLAAVIASELGADVGVSKMGGLLHDLGKAMDHEVEGTHALLGAEFCRRHGVPEIVVN
                     AIASHHHEVEQESVEAVIVESADAISGARPGARRESLEDYLKRVRALEEVANSFEGVA
                     ESYALQAGREIRVIVIPEAIDDLAATRLARTVAKKIEEGMNYPGQIKVTVIRETRSVE
                     YAK"
     gene            3877..4689
                     /locus_tag="VI520_06890"
     CDS             3877..4689
                     /locus_tag="VI520_06890"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLF37642.1"
                     /translation="MAFVLRDAQGREVPVVGRLQIGRDPINQIPIDDPLASRVHATVW
                     EDGRTLFIRDEKSSNGTFVNGKRIQETRLRPRDQIQIGNHVFQVAAAARPVQAVQPGQ
                     PSAPAAANPKGRGCGRWLLAGCLVLILGGCAAGVGGYLAYQGGMLTPDIFLGLVGLGP
                     AAIEVDNFRDDRVYVSIIQLDAPVNSTPLGASLDLNAFDIRTYRVGQPGQYQIDFGSS
                     SGAADLGSCRLGVGSGDHYQFVTLPERIVINRIDNPVSVGTDLIVSTSSLCR"
     gene            4721..5197
                     /locus_tag="VI520_06895"
     CDS             4721..5197
                     /locus_tag="VI520_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37643.1"
                     /translation="MKRIGLLLAGLGAASLTAGCAPSVVVENNGAFTVRAIIGAGGMS
                     DVVSPSPGESSFVEVREGSYTAIVIPDADWIEYAQTTRRLLNEQLANPENMTGEQLLE
                     VFRRLADIAATIDQFQRAGASSAVGAASCSGAVSTEADGSVLVTTTEDGRILIECK"
     gene            complement(5228..>5370)
                     /locus_tag="VI520_06900"
     CDS             complement(5228..>5370)
                     /locus_tag="VI520_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37644.1"
                     /translation="KDVKTTMIYTHVLKRGGFAVKSPLDAETGAAEDRSLRERQASWV
                     TT"
BASE COUNT         1027 a         1485 c         1878 g          980 t
ORIGIN      
        1 tgtccctgct gtgcacgcga tgcggcatat cgttttgagc caacatcaac gataaggggt
       61 ccgcgctacg atgggggacg cggtaggctt cggccaaggc gaaacccacg tgcaggacgc
      121 cacctgcgta agcaggttca aaacctagca gcacacggca cagtgggggc gctctgatag
      181 ccagtgaagg caaccagctt tcactggatt tttttatcca gcgccttggc ccggtcagta
      241 ccaatcgact ggctcggccc tcgtttcttg ctcgcagttc tctcctggat tagacttcct
      301 ttctctacct aacatgccat ctcagacaag tgatcaacgg gaacgagccc tcgaatccgc
      361 agtttccgca atcaagaagc agtttgggga tggggcggtg atgcggatgg gggaggccac
      421 ccatctggcg gttgaaagca tcccgaccgg ttcgctggct ctcgaccagg cccttggggt
      481 cggcggcatt ccccgcgggc gggtgagcga gatctatggg ccggaagcct ccggcaagac
      541 caccctctgc cagcacatcg tggccgagtc tcagcgcttg ggcgggctgg tggcctacat
      601 cgacatggag cacgccctgg atccggccta cgccgccgtc tgtggggtgg acgtcgacaa
      661 cctctacatc gctcagccgg acaccggcga gcaggcgctg gagatcaccg agaccctggt
      721 gcgcagtggg gcgatcgccg tggtggtggt ggacagcgtg gcggctttgg tgccgcgggc
      781 cgagatcgag ggggatatgg gggagccgac catggggctg caggcccggc tgatgagcca
      841 ggccctgcgc aagctctccg gagcgatcaa gcagaccaac accgcggtgg tgttcaccaa
      901 ccagctgcgg cagcggatcg gagtgatgtt cggcaacccg gagacgacct ctggggggat
      961 ggccttgaag ttctacgcct cggtgcgcat agacttacgg cggatcgaga gcatcaaggt
     1021 cgggcaggag gtcgtcggga accgagtgcg ggcccgggtg gtgaaaaaca aggtggccgc
     1081 cccgttccgg gtggccgagt ttgagatcct gttcaatgaa gggatcagca aggttggggg
     1141 gatcctggac ctggcggtcg agaatgggtt ggtggacaag aaaggctcct tctacctcca
     1201 cggcgaaagc cgccttggcc aggggcggga gaatgccaaa cagtatctga gagagaaccc
     1261 cagcttggcg gcggagttgg agaagggggt tcgggcggca tttgccgcca cggccctggc
     1321 ataatagaat tgaccgatca gaccgcccag gcggtcaggc cggtgaactc ggatctcgga
     1381 ttcgaagctt gacaaagcga ttggaatgcg aagaacggag cgaggggtgc agatcgagat
     1441 gagcggcagt tcgctccgga cagatgcgat cgagaggact tgtgaggtgg gcagtgagtc
     1501 aggtcggtgt gcggtttctt tcattgcggc tggcccgccc tggtcgatga ccggtagtga
     1561 ttcgagatag gtcgaattag gccgttccga accttcgcat cggatctatc tcagcttcgc
     1621 ccgtcaagta aggactagag gtcccccaga agtttggggt gttcacacgg ccgtgacccg
     1681 ggtcacggcc tttgtgttta tgcaaggaat gatcaccgcc ttgaagccca ctcgccgcgg
     1741 ccgagtccgg gtctacctgg acgggcggcc cggattggaa ttggcgaagg agctggccgc
     1801 ggggctcgag ctcggcaggc agctcggagc gcagcaggta tctgagctgc aggtccgcga
     1861 tcgagaggaa tccgcctatc ggcaggccgt gcggctgttg gctcgccgcc cgtacaccga
     1921 atgggaacta cgaaggcgct ttgaccgcac cgggttgacc gaacaggagc aagatgcggt
     1981 gatggggagc ctgcgccagg cgacgctggt gagcgatgcg gagtttgcgg cggcctggtt
     2041 ggagaaccgg atggccttcc gaccgcgggg tgcctgggcg ctgcgggagg aactgcgcgc
     2101 ccggggagtt ccggccgagg tcatcgaatc aaccctgagt ggttttgacg agcagcaggc
     2161 cgcactacaa gccgcccgcc agggtttgcg aaagtatcag catttatcgc cggagctgtt
     2221 ccggcagcga ctgagcgcct atttgcatcg gcgcggattc caacaatcaa cgatcggccg
     2281 gctggtggag caactggcgg tcgaccgtaa cgcggaaagc gaggattcga cgtgaatgca
     2341 ccgtggttat tgcccgtggt gggttcgttg gctgcgctcg cgattggttt gaccctcggt
     2401 tacctctttc gccaacttca gcttcggcgg gagctgaagg gccgccaggc ggaggccgag
     2461 cggatcttag aggaggcgaa gggaaaagcc cgagaacttg aattaggggg gcgagacgaa
     2521 gccctgaaat tgcgcgacca ggcggaggcg gaggtctccc gccgccggtc cgacctggca
     2581 aaggaggagg agcggcttca gaagcggcgg gtagatctgg atggacggct ggacaacttg
     2641 gagaagcgtg accagacgat taacaagcgt cagagcgccc tcgacaaacg atccaacgag
     2701 ctggagacga aatacgccga gaccgtggcg gagctggaga gagtctccgg gatgactcgg
     2761 gatgacgcca aagggcagct gctcaaagag gtcgagcgcg aagcgcgggc cgacatggtg
     2821 aaggtcattc atcagatcga agaggaagcc cgggtggagg gcgatcggcg ggcgcgggga
     2881 ctcatcgcag atgccatcca gcgggtggct tcggatcacg tggccgagat tacgacttcg
     2941 aatgtgcccc tgccttccga cgacatgaag ggccggatca tcggccgcaa tgggcgcaac
     3001 atccgggcct tcgaacaggc ggccggagtg gacgtgattg tcgacgacac tccggaagcg
     3061 gtgaccattt catccttcga ttcggtgcgc cgggaagtgg cccgccgctc catggagcgg
     3121 ctggtgctgg acgggcggat ccatccggca aatatcgaga agatcgtgat ccaaacccgc
     3181 gatgaagtgg agcacttgat cgccgaggag ggggagcggg ccgcctacga tgccggcgtt
     3241 ccggggctca gacaagagat caccaagaag ctcggccggc tcaagttccg cacctcctac
     3301 gggcagaacc agctcgctca cgcaatcgag gtcgcccagc tggcggcggt gattgcctcc
     3361 gagctgggtg ccgacgttgg ggtctccaag atgggtgggc tcctgcacga cctcggcaag
     3421 gcgatggatc acgaagtgga gggtacccat gctctgctcg gcgcggagtt ctgccggcgg
     3481 cacggcgttc cggagatcgt ggtcaacgcc atcgcctcac accatcacga ggttgagcag
     3541 gaatcggtgg aggcggtgat cgtcgagtcg gccgacgcga tcagcggcgc ccgaccgggc
     3601 gctcgccgcg aaagcctgga ggactacttg aaacgggtgc gggcgctgga ggaagtggcc
     3661 aattcctttg aaggggtggc cgagagctat gccctgcagg ccgggcgcga gattcgggtg
     3721 atcgtgatac cagaggccat cgacgacctg gcggccaccc gtttggcgcg gacggttgcc
     3781 aagaagattg aggagggaat gaactacccc ggccagatca aggtgaccgt catacgcgag
     3841 acccgttcgg tggagtacgc caagtagcga ttcgcgatgg cctttgtcct gcgggatgct
     3901 caggggcgtg aggttccggt ggtcgggcgg ctgcagatcg gccgcgaccc gatcaaccag
     3961 attccgatcg acgacccgtt ggcttcccgg gttcatgcca cggtctggga agacggaagg
     4021 acgctcttca ttcgggacga gaagagcagc aacgggactt ttgtcaacgg gaagcggatt
     4081 caggagaccc gcttgcgccc gcgggaccag attcagatcg ggaaccatgt gttccaggtg
     4141 gccgcggccg cccggcctgt gcaggctgta cagcctgggc aaccttcggc tccggccgct
     4201 gccaatccga agggccgagg ttgcggccga tggttactcg cgggttgctt ggtgctgatc
     4261 cttggagggt gcgcggcggg agtgggcggg tacctggcct accagggcgg gatgctgacc
     4321 cccgacatat ttctgggtct ggtgggactt gggccggcgg ccattgaggt agacaacttc
     4381 cgagacgatc gcgtgtacgt gtcgatcatc cagctggatg cccccgtgaa ttccacaccg
     4441 ctcggggcca gcttagatct caatgccttt gacattcgca cctaccgagt cgggcagccg
     4501 gggcaatatc agatcgactt tggcagctcg agcggagcag ccgatcttgg cagctgccgc
     4561 ctcggtgtag gctccggcga ccactaccag ttcgtgacgt tgcctgagag gattgtcatc
     4621 aatcggattg ataatccggt gtcggtcggg accgacctga tcgtatccac ttcaagcttg
     4681 tgccgctaag gacctcggtg cgggctacga tgaggtgccg atgaaacgga ttgggctgct
     4741 gcttgcgggt ctcggcgctg cgagcctgac tgccggctgc gcgccgagcg ttgtcgtaga
     4801 gaacaatggt gccttcacgg tgcgcgcgat tattggggcc ggtggaatga gcgacgtggt
     4861 gtcaccttca ccgggggaga gttcgtttgt cgaggtccgg gaggggtcct acacggcgat
     4921 cgtgatacct gatgccgatt ggatcgaata tgcccaaacg acccgcaggc tgttgaacga
     4981 acagctggcg aatccggaga acatgacggg cgaacagctc ctggaggtct ttcgtcggct
     5041 ggcggacatc gcggcgacga tcgatcagtt tcaacgagct ggcgcgagca gtgcggtggg
     5101 cgccgccagt tgttccggcg cggtcagtac cgaggcggac gggtcggtgt tggtcacaac
     5161 gacggaagat ggtcggatcc tgattgagtg caagtaggcg agccgcagtc tttctttccc
     5221 cgctctgtta agtagtaacc caacttgctt ggcgttcgcg gagtgagcgg tcctctgcgg
     5281 ctcccgtttc cgcgtccagc ggactcttga ccgcaaaacc acctcgcttg agcacgtggg
     5341 tgtaaatcat cgtcgtcttt acgtccttat
//
LOCUS       DATDPQ010000309         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1489182, whole genome shotgun sequence.
ACCESSION   DATDPQ010000309 DATDPQ010000000
VERSION     DATDPQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489182"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VI520_06905"
     CDS             <1..318
                     /locus_tag="VI520_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HLF37645.1"
                     /translation="DRAVIFAPVGSLVPVMLAKLRSGGTLATNAIHMSPIPEFPYRLI
                     ARERTLRSVANATYQDGVEFLKLAAAIPVRPRIKLYELEQANQALQDLKAARIEGAGV
                     LIP"
     gene            315..1085
                     /locus_tag="VI520_06910"
     CDS             315..1085
                     /locus_tag="VI520_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLF37646.1"
                     /translation="MIRLGTSGFSYDDWVGPVYPADLPRREWLSHYAREFNSVELNVT
                     FYRVPGQKTVAGWVERTPPDFTFSVKANRELTHERRAPDFPGFRAGIGPLIDSGKLAC
                     ILAQFPHSFHPSPENRLYLEQLRKGLAGLPVVVEFRDRAWVTDQTFAQLSDLGLGYCS
                     VDEPNLPGLMPALATATGPLAYVRFHGRNADKWWEHEQAWERYDYTYSDGELGEWVPK
                     IRGLEAESEVVLVYANNHYRGQSVETIRKLRELLGAAR"
     gene            1173..1928
                     /locus_tag="VI520_06915"
     CDS             1173..1928
                     /locus_tag="VI520_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLF37647.1"
                     /translation="MSGHSKWSTIKRKKEANDARRGSLFTRLAREISIAAREGGGDPE
                     SNFGLRLAVDRARMANMPKDNIERAIRRGTGEDKEGTTFEQVTYEAFAPHGVALLIEM
                     VTDNRNRSVAELRHHVTKYGGNLAEAGAVSWQFKRMAYFAVPAAGLDQDKLFEAAVEA
                     GAEDVIIGREDIEIHAPVAAFKAISDRLKAMGIKPTEARLRMTPSSLVTLPPGDTLQV
                     MRMVEALEEADDVQGVYSNLDVTEEAVALIEPA"
     gene            1929..2450
                     /gene="ruvC"
                     /locus_tag="VI520_06920"
     CDS             1929..2450
                     /gene="ruvC"
                     /locus_tag="VI520_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLF37648.1"
                     /translation="MARPAPGMLVIGIDPGTARTGYGIVRQVENGSLVAVDFGVIETS
                     PELTLPNRLLEISDRLGVLLAEHRPDGGAVERLFLREHVNTALSVGQASGVALLALAK
                     AGIPIKEYSPLEVKQAICGYGRAEKRQVQDMVKAVLGLSERPRPDDAADALAVAICHV
                     HSMPIGGRLEAEQ"
BASE COUNT          484 a          758 c          840 g          467 t
ORIGIN      
        1 gaccgggcgg taatcttcgc accggttggc tcgctcgttc ctgtaatgct ggccaagctc
       61 cgcagcgggg gcaccctggc tacgaacgcg atccacatga gtccgatacc cgaattccct
      121 tatcggctga tcgctcggga gcggaccctc cgcagcgtcg ccaatgccac ttaccaggac
      181 ggtgtggaat tcctgaagct cgcagcggcc atcccggttc ggcctcggat taagctctac
      241 gaactcgagc aggccaacca ggcgctgcag gacctcaagg ctgccagaat cgaaggcgcg
      301 ggagtcttga tcccatgatt cgcctcggaa cctccgggtt ctcctatgat gattgggtgg
      361 gtccggtcta cccggcggat ctgccgcgcc gggagtggct ttcccattac gccagggagt
      421 tcaacagcgt tgaactcaat gtcacgttct atcgagtgcc ggggcagaag accgtggcgg
      481 ggtgggtgga gcgcacgcct cccgatttca ccttttcggt gaaggccaac cgcgaactca
      541 cccacgaacg tcgcgcaccc gacttcccag gcttccgggc tgggattggg ccgctcatcg
      601 attcgggcaa gctggcctgc attctcgccc aattccctca ctcgtttcat ccgagccccg
      661 aaaaccggct ctatctggag cagctacgca agggactggc ggggctcccg gtcgtggtgg
      721 agttccggga tcgagcctgg gtcacggatc agactttcgc ccagctttcg gacttggggt
      781 tgggttactg ctcggtcgat gaaccgaacc tgcccgggct gatgccggcg ctggctaccg
      841 cgaccggtcc gctggcctac gtgcggttcc acgggcgcaa cgccgacaag tggtgggagc
      901 atgaacaggc ctgggaacgg tacgactaca cttattccga cggtgagctg ggcgagtggg
      961 tcccgaagat ccgcgggttg gaagccgaga gcgaagtcgt attggtctat gccaacaacc
     1021 actaccgcgg ccaaagtgta gagaccatcc gcaagctgcg ggaactgctg ggtgcggccc
     1081 gttgaccgac tgcaccttga tttggaggct gtcagcccgg ttggtataat cgggtggcct
     1141 gcggaatcct cttccggtta gagggcaaag ctatgtccgg acactcaaaa tggtccacga
     1201 tcaaacgaaa gaaggaagcc aacgacgctc ggcgcggcag tcttttcacc cgcctggcgc
     1261 gtgagatttc catcgcggcc cgcgaaggcg gtggagatcc ggaaagtaac ttcggcttgc
     1321 ggctggctgt ggatcgggct cggatggcca acatgcccaa ggacaatatc gagcgggcga
     1381 tccggcgggg caccggcgag gacaaagagg gaactacctt cgagcaagtc acctatgagg
     1441 ccttcgcacc gcacggggtt gcgctgctga tcgagatggt cactgacaat cggaatcgga
     1501 gtgtcgccga gctgcgccac cacgttacta agtacggcgg aaacctggca gaggccggcg
     1561 cggtatcgtg gcagttcaag cggatggcct atttcgcggt cccggccgcg ggcctggacc
     1621 aggacaaact cttcgaagct gcggtggaag ccggagcgga ggatgtgatc atcggccggg
     1681 aggacatcga gattcatgcc ccggtggccg ccttcaaggc gatcagcgat cggctcaagg
     1741 caatgggaat caagccgacc gaagccaggc tgcgcatgac gccgagctcg ctggtgaccc
     1801 tgccacccgg ggatactctg caggtcatgc ggatggtcga agccttggaa gaggcggacg
     1861 acgttcaggg ggtgtactca aacctggacg tgaccgaaga ggccgtcgcg ttgattgaac
     1921 ccgcctgagt ggcgagacct gcccccggca tgctggtcat cggcatcgac ccaggaaccg
     1981 cccgcacggg ctacgggatc gtacggcaag tcgagaacgg atcgctcgtc gcagtagatt
     2041 tcggggtaat cgaaaccagc cccgagctca ctctccccaa tcggttactt gaaatctcag
     2101 atcgactcgg cgtgctgctg gctgagcacc gcccggatgg gggtgccgtg gaaagactct
     2161 tcttgcgcga gcatgtgaac acggccttaa gcgtcggaca ggcctccggg gtcgcactgc
     2221 tcgccctggc taaagccggg attccgatca aggagtactc gccgcttgag gtcaagcagg
     2281 ccatttgcgg gtatggcagg gcggagaagc gccaggttca ggatatggtg aaagccgtgc
     2341 tcggattgtc ggagcgtcca cgccccgacg acgcggcgga cgcattggcg gtggcgatct
     2401 gccacgttca ctcgatgccg atcggcggcc ggctggaggc cgagcagtga ccctcccatg
     2461 atttcctcgc tgcgtggaat cgttcaggcc gtcgcggagg actcattggt gctcgaggtc
     2521 ggcggggttg ggctgcatat cgcggcccc
//
LOCUS       DATDPQ010000310         8067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2519788, whole genome shotgun sequence.
ACCESSION   DATDPQ010000310 DATDPQ010000000
VERSION     DATDPQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519788"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /gene="fusA"
                     /locus_tag="VI520_06925"
     CDS             complement(<1..1389)
                     /gene="fusA"
                     /locus_tag="VI520_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLF37649.1"
                     /translation="MPREFPLERYRNIGIIAHIDAGKTTTTERVLFYTGRTHRMGSVD
                     EGTTVTDWMDQERERGITIVSAAVTASWRDFVINIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQTETVWRQADRFGVPRICFVNKMDRTGASYERTIESIRRRLGAQPL
                     AMQLPIGSESNFSGVVDLLDDVAVIYSEEPGASPELEPVPAELRGLAAEQRTELIERI
                     AETDDGLTERFLEGQPIPTSELKAALRRAVIAGKLTPVFAGSSLKNRGVRRLLDAVVD
                     YLPSPLDIPPLRGLKPGTEVEITLEAKDDAPLSALVFKIVSDPYVGRMAYLRVYSGTL
                     TKASSVYNASKDRKERVGRLIRMYADHREDVDEVMAGDIAAVLGMKFAFTGDTLCDPA
                     YPIVLERIIFPEPVIFTAIEPKTKGDQDRLGDALQKLAEEDPTFRVGTDENTGQTMIW
                     GMGELHLEVLVDR"
     gene            complement(1401..1868)
                     /gene="rpsG"
                     /locus_tag="VI520_06930"
     CDS             complement(1401..1868)
                     /gene="rpsG"
                     /locus_tag="VI520_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLF37650.1"
                     /translation="MRRTKPERRTTTGDIRYNNPAIHGLINRMMKRGKKSTAIRIVYD
                     AFEMIEQRVKRNPVEVFEEAIKNTSPTLEVKPRRVGGATYQVPVEVQFERRTSLAVRW
                     ILAAARTRPGKTMAEKLAGELMDAAAGTGSAIRKREDTHKMAEANRAFAHYRW"
     gene            complement(1944..2363)
                     /gene="rpsL"
                     /locus_tag="VI520_06935"
     CDS             complement(1944..2363)
                     /gene="rpsL"
                     /locus_tag="VI520_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HLF37651.1"
                     /translation="MSTINQLVRKGRKSKRSKTKAPALQYTFNSMNQRRELQPRGAPQ
                     KRGVCTQVRTQTPKKPNSALRKVARVRLTNGLEVTAYIPGEGHSLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTMDTTGVDGRRQGRSKYGGKRPRE"
     gene            complement(2647..4452)
                     /locus_tag="VI520_06940"
     CDS             complement(2647..4452)
                     /locus_tag="VI520_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37652.1"
                     /translation="MQPPTKVPKPRDWLNPLSVGVGCLFAAALFALGRAGPDFDRYLD
                     WSRAFARGDILELRGSILSPNNVPLTQWSHGAGLILALPQILTGETNGPGLSVLVAAW
                     LAGVVFWWAMFRLIVRAAAGIVSLTIFGMGAAFLGTHAGFYSHTYGSESVGYALGAVL
                     ALTLVDGRPWRMWEALVGGAAATLLFVLRSNLAVFALPATLVLGARAVAARRVVGPRK
                     VILALTWLAGTSLIGILQVALVNHWMTGDYLRSAYVFGADGFHSVELAKPQLLAVLAH
                     PWYGLLSYHPLYAIGMAAVIALVLRPGPRRARLGWLAIGVLVMVQLYVQASWYVWWQG
                     TGTFGQRGMSISAVLLVPALVIVIERSPSGRRLGRNLWILLTLLGCLWSYLLLLQGET
                     SFYSTVDLMEAQLQELALLARPAMLLPLLGGLILPGGIIVWLLRRNRAQRPDSLRDWA
                     AGLLGILSLSYLELELAGGSLERVAVFGLAAFALAALVLLSNWAISRWNVERSTVLQG
                     QESAYSVVGAGLVTTFLATAVLFARLAVRTEQRIGREEPAPRAVACSSSVQWDEVRES
                     FYEYQDVEGFETQRAGLETFLVRSGGVECPELALP"
     gene            complement(4518..>8067)
                     /gene="rpoB"
                     /locus_tag="VI520_06945"
     CDS             complement(4518..>8067)
                     /gene="rpoB"
                     /locus_tag="VI520_06945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLF37653.1"
                     /translation="IMTDKGTFVINGTERVVVSQLIRSPGVYFEAPEDRTTGRRLASA
                     KLIPDRGAWMEFESRKSNYLTLKFNRKRTIPVTILLRALAAINDGTKKPAIKEGSDEE
                     LLQLFAQADVDPDHPYIATTMRQEPTWERRDNRSVAEEALVEFYRRMRPGDPPTLDNA
                     REYLRDQLIDPRRYDLERVGRYKLNGKLGLEKFVAEDHRQVTSWDVVRIVERMIQINN
                     GAADPDDIDHLGNRRVKTVGELIQSKLRVGLRRMERVILERMSIRDQEQLSPVSLINI
                     RPVVAAIREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRDVHHS
                     HYGRICPIETPEGPNIGLIGRLASYARVNRFGFIETPYRRVVNELSSKDKRLVGKRLR
                     GGWKDAKGEKVLAKDGTVVSESLASRIARRKAQIPVVPFVSDDTQYLSADEEDKYVIA
                     QANTPLNERLEFANPRVSCRRHGDFFFATPDQIIFMDVAPNQIVGVSASLIPFLEHDD
                     ANRALMGSNMQTQAVPLIDPEVPLVSTGMERQAAYDSGQVILAEESGEVESVSGSTVV
                     VRSKGASRTYKLRKFNRSNQSTCIDQRPSVIKGQRVTKGDVIADSTSTEGGELALGQN
                     VLCAFLSWEGGDFEDAILISERLVQQDRYTSIHIEKHEVEARDTKLGPEEITFDIPNV
                     GDEALRDLNEDGIVRIGAEVGPNDILVGKITPKGEKELTPEEKLLRAIFGEKAREVKD
                     TSLRMPHGERGKVVDVKVFTREEHRDLPPGVEKMVRVSVAQKRKATEGDKMAGRHGNK
                     GVISKVVPIEDMPFLADGTPVDLILNPLGVPGRMNIGQILEAHLGWAANRLGFRAITP
                     VFDGADEEEIEAELARAWLIDRAWQEIGTAAWDWVKEKGYYDEEAFEDDEEIRRLYLE
                     NWLKAKRYDIDLIATDRNYARRVALREWLRQKGYDPDKLLVFDDSETPVDDREPFDQA
                     VIDVCLRLWIESKGRKIKPDSTPEAVAKQAIKVMAETHEPAPTLGKQALFDGKSGEAY
                     DQPVTVGVVMMMKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALE
                     AYGAAHTLQEMLTVKSDDVQGRVKTYEAIVKGESVEEPGIPASFRVLVKELQSLGLSV
                     EAINDGGDIVRFGKQDDRTRPPRLGLGLLDLDTEPR"
BASE COUNT         1497 a         2669 c         2336 g         1565 t
ORIGIN      
        1 gcgatccacc aggacttcga ggtgcagctc gcccatcccc caaatcatgg tctggccggt
       61 gttctcgtcc gtgccgaccc ggaaggtcgg gtcctcctcg gccagcttct gcagcgcgtc
      121 ccccagccgg tcctggtcgc ccttggtctt gggctcgatg gcggtgaaga tgaccggctc
      181 cgggaatatg atgcgctcaa gcacaattgg gtaggccggg tcgcacaagg tgtcgccggt
      241 gaaggcgaac ttcatcccaa gcacggccgc gatgtcaccg gccatcacct catcgacatc
      301 ctcgcggtga tcggcataca tccgaatcaa acgcccaacc cgttccttgc ggtctttgct
      361 ggcgttgtaa accgaggagg ctttggtgag cgtgccggaa tagacccgca ggtatgccat
      421 ccggccaacg tatggatccg agacgatctt gaagaccagc gcgctcagcg gagcgtcatc
      481 tttggcttcc agagtgatct cgacctctgt gccgggcttc agtccgcgga gcggcgggat
      541 gtccagcggc gagggcaggt agtcaaccac cgcgtccagc agccgccgca cgcctcgatt
      601 cttgagcgac gagccggcga agaccggggt cagcttgccg gcgatgactg cccggcgcag
      661 ggcggctttc aattccgagg tcggaatcgg ttgcccttcg agaaatcgct cagtcaggcc
      721 atcgtcggtc tcggcgattc tttcgatgag ttcggtccgc tgctcggccg ccagcccccg
      781 caattcggcg gggaccggtt cgagctccgg cgaggcgccc ggttcctcgc tgtaaatgac
      841 agcgacgtca tccagtagat cgacgacgcc gctgaagttc gactcgcttc cgatcggcag
      901 ctgcatggcg agaggctggg cccccagccg ccgacggatc gattcgatgg tgcgctcgta
      961 ggaggcaccg gttcgatcca ttttattgac aaaacaaatg cgcggcacac caaaccgatc
     1021 ggcctgccgc cagacagtct cggtctgcgg ttcgaccccc tggaccgcgt caaagaccac
     1081 aaccccgccg tccagtaccc gcagggagcg ctgcacctcg gcggtaaagt cgatatggcc
     1141 cggcgtgtcg atgatattga tgacgaaatc ccgccagctg gcagttaccg cggccgacac
     1201 gatggtgatg cctcgttccc gctcctggtc catccagtcg gtcaccgtcg tgccttcatc
     1261 caccgatccc atccgatggg tgcggccggt gtagaacagc acccgctcag tagtcgtggt
     1321 ttttccagca tcgatgtggg cgatgatgcc gatgttgcgg tagcgctcaa gcggaaattc
     1381 gcgcggcatg ccgtcggttc ctaccagcga tagtgggcga aggctcggtt ggcctccgcc
     1441 atcttgtgag tgtcttctcg tttgcgaatg gcagagcccg tcccggctgc cgcatccatc
     1501 agctctccgg ccagtttttc cgccatcgtc tttcccggcc gggtacgggc cgcggccagg
     1561 atccagcgga ccgccaggga ggtcctccgc tcaaactgga cctcgactgg aacttgatag
     1621 gtcgccccac ccactcgacg cggtttgact tccagggtcg gcgaggtgtt cttaatcgcc
     1681 tcctcgaaga cttcgaccgg attgcgcttg acccgctgct caatcatctc gaaggcgtca
     1741 tacacgatgc gaatggcagt gctctttttg ccgcgcttca tcatccggtt gatcaaaccg
     1801 tggatggccg ggttgttgta gcgaatgtcg ccggtcgtcg tccgtcgctc tggtttcgtc
     1861 cttcgcatcc tcagtctccg ttcaacacac aagcctcggc cgccgcgcct gcggcaaccg
     1921 agcagttcaa tgaattgcgc tacctactct cgagggcgtt tgccgccgta cttcgagcgg
     1981 ccctgccggc gcccatccac ccccgtagta tccatcgtgc ctcgcacgat gtggtagcga
     2041 acgccgggca ggtccttcac tcgtccgccg cgcaccagaa cgacggagtg ctcttgcaag
     2101 gagtgcccct cccccgggat ataagccgtc acttccaggc cgttggtgag ccgcactcga
     2161 gcgaccttgc gcaaggccga gttcggcttc ttcggtgtct gggttcggac ctgggtacag
     2221 accccccgct tttgcggggc gccgcgcggt tgcaactccc ggcgctggtt catcgaattg
     2281 aacgtgtact gcagcgcggg tgctttggtt tttgagcgct tgctcttgcg gcccttgcgg
     2341 accagttgat tgatcgttga caaagactgt ccctttacac aaatccccgg ggcaggtgcc
     2401 ccgggtcact cgacgcaaag gccccgtatt cgccagggcc tcgaaggact acgatgaatt
     2461 cgagctgcgc aaaccgcgca gctcgagctg cccagactgt cgaactgaaa ttctaccaaa
     2521 cggccacggc gccgtcaagg aaaactgtgg gcctctaaag ggcaccccag cccgccaggg
     2581 gggctgcttc gccgctcagc taaggcccca actctgtcct ctgccggaca gcaatcaacg
     2641 attcgactaa ggaagcgcta gctctggaca ctcaactccc ccgcttcgaa cgagaaaggt
     2701 ctccaacccg gctctctggg tctcaaatcc ctccacgtcc tggtactcat agaagctttc
     2761 ccgtacttcg tcccattgga cactgctgct gcacgccaca gcccgcggcg ctggttcctc
     2821 cctcccaatt ctttgctcgg tgcgaaccgc cagccgggcg aagagcactg ccgttgccaa
     2881 gaacgtcgta accaagcccg caccaaccac gctatacgcc gattcctgcc cctggagcac
     2941 agttgaccgt tcgacattcc atcgactgat tgcccaattg gaaagcaaga ccagcgccgc
     3001 cagagcgaag gccgccaagc cgaagaccgc gacccgctcg agcgatccgc cggcgagctc
     3061 taactccagg taactgaggc tcaagatccc gagcaggccg gcggcccaat ccctcaacga
     3121 gtctgggcgc tgagcccgat tccggcggag cagccagaca atgattcccc ccgggaggat
     3181 cagccctccc agcagcggca agagcatcgc tgggcgggcg agcagtgcaa gttcctggag
     3241 ttgggcctcc atcaggtcaa cagtgctgta gaaggaggtc tccccctgca acaagagcaa
     3301 gtacgaccac agacagccaa gcagggtcag cagaatccag agatttcttc caagcctccg
     3361 accggatgga gaccgctcaa tcacgattac cagggccggg accagcagga ccgcgctgat
     3421 cgacattccg cgctgcccaa aggtcccggt tccctgccac cacacgtacc aactggcctg
     3481 aacgtagagt tgaaccatga ccagcacgcc aatggccagc caacccagcc ttgcccgcct
     3541 cgggccgggc cgcaaaacca atgcgatgac tgccgccatc ccgatcgcat acagcggatg
     3601 gtaggacagc agcccgtacc agggatgggc gagcacggcc agcaactgtg gcttggcgag
     3661 ctcgaccgaa tgaaagccgt ctgctccgaa tacgtaggcc gatcgaaggt agtcgcccgt
     3721 catccagtga ttgaccagtg cgacttgcag gatcccgatc aggctggtcc ctgccaacca
     3781 ggtcaacgcc aggatcactt tccgtggccc gacaacccgc cgcgcggcaa ccgcccgagc
     3841 gccgagcacc agagtcgcgg gcagcgcaaa gaccgccagg ttggatcgca acacgaagag
     3901 cagcgtggcc gccgcgcctc caaccaatgc ttcccacatt cgccagggcc gcccatcaac
     3961 aagagtgagg gccagaaccg cacccagggc gtacccgacc gactccgatc cataggtatg
     4021 ggagtagaaa ccggcatggg ttccaaggaa ggcagccccc attccaaaga tcgtcagcga
     4081 aacgattccg gccgcggcgc gaacgatcag ccggaacatt gcccaccaga acaccacgcc
     4141 ggccaaccag gctgcgacaa gcacactcag ccccgggccg ttcgtttctc cggtgagaat
     4201 ctggggcagg gccaggatca gaccggcgcc atgcgaccat tgagtcagcg ggacgttgtt
     4261 gggggaaagg atcgagccac gtaactcgag aatgtcgcct cgggcgaagg ccctgctcca
     4321 atccaggtag cgatcgaagt ccggcccggc ccgcccgagg gcgaaaagtg ccgcagcgaa
     4381 taggcaaccc actccgactg agagcgggtt cagccaatcg cgaggcttgg ggaccttcgt
     4441 cggcggttgc acttcagttc cagaatgaaa tggcgcccgc aatgcgggcg ccacagtttc
     4501 acagtccgtc cttccgatca gcgcggctcg gtgtcgaggt ccagcagtcc gagcccaagt
     4561 cgcggcggcc gagtgcggtc gtcttgcttc ccaaatcgca ctatgtctcc gccgtcgttg
     4621 atcgcttcca ccgagagacc aaggctctgc agttccttga ccaagactcg gaacgaggcc
     4681 gggatccccg gctcctctac cgactcgccc ttgacgatgg cttcataggt cttgacccgg
     4741 ccctgcacgt catccgactt cacggtcagc atctcttgca aggtatgggc cgcgccgtac
     4801 gcctccagcg cccagacctc catctctccg aagcgctgtc cgccgaactg agcctttccg
     4861 cccagcggct gctgggtaac cagcgagtac gggccagttg aacgcgcatg caccttgtct
     4921 tcaaccaggt ggtgcagttt catcatcatg accactccga ccgtgaccgg ttgatcgtag
     4981 gcttcaccgc ttttgccgtc aaacagagcc tgcttcccga gggtcggcgc cggctcgtgg
     5041 gtctcggcca tcaccttgat cgcctgtttg gccacggctt cgggggtgct gtcgggcttt
     5101 atcttgcggc ccttggactc aatccacaac cgcaagcaaa catcgattac tgcttggtcg
     5161 aacggctcgc gatcatcgac cggggtctcg gagtcatcga agacaagcag cttatccggg
     5221 tcataaccct tctgccgaag ccattctctc agcgccaccc ggcgggcgta attccgatcg
     5281 gtggcgatca gatcgatgtc gtagcgcttc gccttcaacc agttttccaa atacagacgc
     5341 cgaatctcct cgtcgtcctc gaaggcttcc tcatcgtagt agcccttctc cttcacccag
     5401 tcccaggccg cggtgccgat ttcctgccag gcccgatcga tcagccaggc cctggccagc
     5461 tcggcctcga tctcttcttc atctgcgccg tcgaacaccg gagtgatggc tcggaatccg
     5521 agccgattgg ccgcccaacc caaatgggcc tcgaggatct ggccgatgtt catgcgcccc
     5581 ggtacgccca gcggattcag gatcaggtcc accggggtcc catccgccag gaacggcatg
     5641 tcctcaatcg ggacgacctt ggatatgaca cccttgttgc cgtgccggcc ggccatcttg
     5701 tcgccttcgg tcgccttacg cttctgggcc accgagactc ggaccatctt ctccaccccg
     5761 ggaggcagat cccggtgctc ctcgcgggtg aagaccttga cgtcgacgac cttgccgcgc
     5821 tcgccgtgcg gcatgcgcag ggaggtgtcc ttcacctcgc gggccttctc gccgaagatc
     5881 gcccgcagca gtttctcttc gggagtgagc tctttctcgc ctttgggagt gatcttgcct
     5941 accagaatat cgttgggtcc gacctccgcc ccgatccgga caatcccgtc ctcatttaga
     6001 tcgcgcaagg cttcatcgcc aacgtttgga atatcgaagg tgatctcctc gggaccgagc
     6061 ttggtatccc gggcttcaac ctcgtgcttc tcgatgtgga tcgaggtgta gcgatcctgc
     6121 tgcaccaatc gctcggagat cagaattgcg tcctcaaaat cgccgccttc ccaggagagg
     6181 aaggcgcaca gcacgttctg ccccaaagcc agctcgccgc cctcggtgga ggtcgagtcg
     6241 gcaatgacat cccccttggt cacccgctgg cccttgatca ccgatgggcg ctggtcgatg
     6301 caggtgcttt gattggaacg attgaacttg cgcagcttgt aggtccgact ggcgccctta
     6361 gaccggacaa ccaccgtgct gcctgaaact gattcgactt ccccactctc ttcggccagg
     6421 attacctgcc ccgaatcgta ggccgcctgc cgttccattc ccgtggaaac cagcggcact
     6481 tccgggtcga tcaggggaac ggcctgggtc tgcatattgg agcccatcaa ggcccggttc
     6541 gcatcatcgt gctccaggaa tggaatcaag gaagcactga ccccgacgat ttggttggga
     6601 gccacgtcca tgaagatgat ctggtcgggt gtggcgaaga agaaatcacc gtgacggcgg
     6661 caggaaactc gaggattggc aaactccaac cgctcgttca gcggggtgtt cgcctgggca
     6721 attacgtatt tgtcttcctc gtcggccgac aagtactgcg tgtcatcgga gacaaacgga
     6781 acgaccggga tttgggcctt gcgtcgcgcg atcctgctcg ccaggctctc gctgaccacg
     6841 gttccgtcct tggccaggac tttctcgccc ttggcgtcct tccaaccccc ccgcaagcgt
     6901 tttccaacca accgcttatc tttggagctc agctcgttca ccacccggcg atagggggtc
     6961 tcgatgaagc cgaagcgatt taccctggcg tacgaagcca gccttccgat caggccgatg
     7021 ttggggccct ccggggtttc gatcgggcaa atccggccgt agtgggagtg gtgcacgtct
     7081 cgcacatcga agccggcccg ttcccgccgc agaccgccgg gccccaaggc agacaaggtc
     7141 cgtttgtgac gcagctccgc cagcggattg gtctgatcca tgaattggga gagctggctt
     7201 gaaccaaaga attcccggat cgcggccacg accggccgga tgttgatcaa tgaaaccgga
     7261 gagagctgct cctgatcccg gatcgacatc cgctccagaa tgacccgctc cattcggcgc
     7321 agcccaaccc gcagcttgct ctgaatcaat tcaccgaccg ttttcacccg gcgattgccg
     7381 aggtggtcga tgtcatcggg atccgcggcc ccgttgttga tttggatcat tcgctcgacg
     7441 attcggacca cgtcccaact ggtgacctga cgatggtctt cagccacgaa tttctccaag
     7501 ccgagtttgc cgttcagctt gtagcggccc acccgctcca ggtcgtagcg ccgcgggtcg
     7561 atcagctggt ctcgcaggta ctcgcgtgca ttgtcgaggg tcggcgggtc accggggcgc
     7621 atccggcggt agaactcgac cagcgcctcc tcggccacgc tgcggttgtc gcggcgttcc
     7681 caggtcggct cctggcgcat ggtggtggcg atgtacggat gatcgggatc gacatcggcc
     7741 tgggcgaaga gttggagcag ctcctcgtct gacccttctt tgattgccgg cttcttggtg
     7801 ccatcgttga tggcggccag cgcccgcagc aggatcgtga ccgggatcgt ccgcttgcgg
     7861 ttgaacttta gagtcaggta attgctcttg cggctctcga attccatcca agccccgcgg
     7921 tcggggatga gcttggccga cgccaggcgc cggccggtcg tccgatcctc aggagcctcg
     7981 aaatacacgc ccggggagcg aatcagctgc gagaccacca cccgctcggt cccgttgatc
     8041 acgaaagtgc ccttatcggt catgatc
//
LOCUS       DATDPQ010000311         6529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2787125, whole genome shotgun sequence.
ACCESSION   DATDPQ010000311 DATDPQ010000000
VERSION     DATDPQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787125"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VI520_06950"
     CDS             complement(<1..284)
                     /locus_tag="VI520_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37654.1"
                     /translation="MTLETGSILNNRYRIEGHLGKGGMGTVYLAYDQTLELRVALKEN
                     LNPHPESEQQFRREAKLLASLRHPNLPRVTDHFILSEVQYLVMDYIEGEDL"
     gene            423..941
                     /locus_tag="VI520_06955"
     CDS             423..941
                     /locus_tag="VI520_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37655.1"
                     /translation="MDYYVIVLRIIHILGGVFWAGASFALVGFVSPAAAQAGPAGGQF
                     MQRLVLGTRWRFVISAAAGLAVLTGLLLYWRASGGLRPEWLSTGTGIAFTIGGLAGVT
                     AMVFGFQVGNSSSQLAELGQRIGSAGRPPSPEDAAGLAALQARLQSLGTVTAVLLVIA
                     LLGMSIARYISF"
     gene            1089..2717
                     /locus_tag="VI520_06960"
     CDS             1089..2717
                     /locus_tag="VI520_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLF37656.1"
                     /translation="MKPIRNFRHPVLMLTGLLLLALACNLPGSTPDEPTAAPPPADQQ
                     ATAVPKEPEEPTGRTGAVSSLEDVKQAVVQIVAEGTFIDPEFGLQVNAAGSGSGFIID
                     PSGIAVTNNHVVTGAALLKVYVAGEQEPRNARLLGVSECKDLAVIDIEGDGFAYLDWF
                     DGTIGVGLQVYAAGFPLGDPEFTLTQGIVSKAQADGESDWASVNSVLEHDATINPGNS
                     GGPLVDGNGRIVGINYASSSLFSQYFAISRDEATGAIDQLQAGENVESIGVNGVAVAS
                     EDGTLSGIWVASVASGSAADEAGVLPGDIITTLEGLVLATDGSMADYCDILRTKGDDS
                     TMSIEVLRYQQGEYLTGQLNGRPLETAGVFGQQLAGDVPDTGSGYLEYTQVTDDYGAI
                     VVEVPSNWIDIDGSAWVDAGDVFGASISAAPDLDGFFGTWDTPGVFFAASDDLARWGG
                     YVQLLDFYRPDFAAVCELDGRYDYDDGYYRGKYDYFVKCGGSGGADFLILTAVPIDTS
                     VKLLILVEVQILTNDDVDAADRILATFDVVGALP"
     gene            3084..4130
                     /locus_tag="VI520_06965"
     CDS             3084..4130
                     /locus_tag="VI520_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLF37657.1"
                     /translation="MSGEFVLRIIGLLVLAIAGVIVGVRLSIPAGGPAELWAGVLGAV
                     GALLRLVATPFVTTRPLRAIRAGVAQVSAQTMVAGLSGLIVGLLAAGLVAFPISLLPT
                     PFGQLLPFGAAIVFSYLGVTVFIMRQNDIANVWRGRFQSSGDQGGGGPGRSILLDTSV
                     IIDGRIADIARTGFIQGPMLVPSFVLIELQHIADSGDPLRRQRGRRGLDILNRLQKDN
                     PIPLRITDLDVEGVREVDDKLVILAKQLRSPILTNDYNLNRVAELQGVTVLNINELAN
                     AVKAVYLPGEKIEVQVIQEGKEFGQGVGYLDDGTMVVVEEGKSNIDHTITVTVTKVLQ
                     TAAGRMIFARPDSQ"
     gene            4164..5564
                     /gene="cysS"
                     /locus_tag="VI520_06970"
     CDS             4164..5564
                     /gene="cysS"
                     /locus_tag="VI520_06970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLF37658.1"
                     /translation="MSRKKEPFETVVEGQVRMYVCGPTVYDQAHIGHAMSSIVFDVIR
                     RYLEYRGYQVRHVMNYTDVDDKIIARSKELGQDPIQLAQHYVEQYDRHLQELNLLPPD
                     VRPRVSDEIDGIVGMVKELIEAGHAYRVNGDVYFDVGSDPHYGKLSGRKLDEMQAGAR
                     LEIDERKRDPADFALWKTAKPGEPSWESPWGRGRPGWHIECSAMSLHHLGDQIDIHGG
                     GNDLIFPHHENEIAQTESLTGQQFSRYWVHNGMMQLNGETMSKSTGNMLPIAEFLQQS
                     EADVLRLIVLNSHYRTPLAFNSEIAEAAARGLDRLRTAMKPDQATGDGGLTGEGDPGS
                     SEVEKLLAQAVERAQQDFRVSMDDDFNTPQALSSLYELVRSVNQARDAGVSQAALVAA
                     QEMLGELGGVLGLRLASGTTPDPKLAALVEMLVEIRDELRAAEQWELADRIRQRLADQ
                     DILLEDGKHGTSWRQG"
     gene            5539..6393
                     /gene="rlmB"
                     /locus_tag="VI520_06975"
     CDS             5539..6393
                     /gene="rlmB"
                     /locus_tag="VI520_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HLF37659.1"
                     /translation="MARVGGRAKPRPRTFSGDLSETIWGRHPVEEVLRAGRRKVHRLF
                     VLQDHPPPERVLRLAKEAGVRLQPVELLPVTSQQHQGIAAQVDPYPYAELNSVLERAA
                     RAGEAPLLLLLDQVQDPQNLGTLLRTAEAVGVHGVVLPLNGAAGVTPAVVAASAGACE
                     HLWIARANLAQAIRRLKQEGLWIAGLENSEQAKRIDQIDLRPPLALVVGSEGRGLRRL
                     VGESCDFLVQIPMRGLVESLNAAVAGSVVLYEVWRRAGYSGASGGGVPQSDRHAPEPS
                     RSSKNAND"
BASE COUNT         1212 a         1859 c         2182 g         1276 t
ORIGIN      
        1 aggtcctcgc cctcgatata gtccatcacc agatactgaa cttcggacag gatgaaatga
       61 tcggtcaccc ggggcaggtt gggatggcga aggctggcca gcagcttggc ctcccgccga
      121 aattgctgtt cggattcagg gtgggggttg aggttctcct tgagcgcaac cctcagctcg
      181 agagtctgat cataggcgag gtagaccgtg cccattccgc ccttgccgag atgcccctca
      241 atccgatagc ggttgttgag gatcgatcca gtctctagag tcacggcggg gattgtagtg
      301 tagttcgcga aagtgggtca attccccgct aggtccagcc ctagatggaa tgtgggctcg
      361 gaatataatc tgacggtcgc acgcggtcgt cgcggccgca atctcggcga cggagccagc
      421 agatggacta ctatgtgatc gttttacgaa tcatccacat cttgggcgga gtgttttggg
      481 ccggggcgag cttcgccttg gtcggctttg tctccccggc cgccgcgcag gccgggccgg
      541 ccggaggcca gttcatgcag cgattggtgc tcggaacacg ttggcggttc gtgatctcgg
      601 ccgcggccgg actggcggtc ctgaccggtc tgctgctgta ctggagggcc tccggggggc
      661 tacggccgga gtggttgagc accgggaccg ggatcgcttt cacgatcggc ggcctggctg
      721 gcgtgaccgc tatggtcttc ggattccagg tagggaacag cagcagccag ctggcggagt
      781 tagggcagcg gatcgggtcg gcaggtcgac ctccctcccc ggaagacgcg gccgggctgg
      841 cagccttgca ggctcggctg cagagcctgg gcactgtcac ggcagttctg ctggtgatcg
      901 ccttgttggg catgtccatt gcccgctaca tctccttcta gggccgacgc gattcgggtc
      961 cttgggcccg accggcgaga ccgaatctgt aaggaaattg caaggtctgc tgattggtgg
     1021 cttctgccga gttcctctat actagggccc ttgagctgtg gcccacatcc ttcgacaggg
     1081 gtacatccat gaaacctatc cgtaacttcc gccatccggt gctgatgctg accggcctgt
     1141 tgcttttagc cctggcctgc aacctcccgg gctccactcc ggacgagccg accgcggctc
     1201 cgccgccggc cgaccagcag gctactgccg tgccgaagga gccggaagag ccgaccggac
     1261 ggactggagc cgtctccagc ttggaagacg tcaaacaggc cgtcgtccag attgtcgccg
     1321 aaggaacgtt tatcgaccct gagtttggct tgcaggtgaa cgcggccggc agcggctcgg
     1381 gtttcatcat cgatccttcc gggatcgcgg tcaccaacaa ccatgtggtg accggggcag
     1441 ccctgctgaa ggtatatgtc gccggcgagc aagagcctcg caatgccagg ctgctcggcg
     1501 tgtcggagtg taaggatctg gcggtcatcg acatcgaagg ggatggcttc gcttacctgg
     1561 attggttcga cggcacaatt ggcgtcggcc tgcaggtcta cgccgccggc ttcccgctgg
     1621 gcgaccccga attcacgctg acccaaggga tcgtctcgaa ggctcaggcg gatggcgagt
     1681 cggactgggc ttcggtgaat tcggtcttgg agcacgacgc caccatcaac ccaggcaact
     1741 cgggcggtcc gctggtcgac ggcaatggcc ggatcgtggg cataaactac gccagttcaa
     1801 gtttgttctc tcaatatttc gccatctccc gggatgaagc cacgggcgcg atcgaccaat
     1861 tgcaggctgg agagaatgtc gaatcgatcg gtgtgaacgg agtggccgtc gccagtgaag
     1921 acgggaccct ctcggggatt tgggtggctt cagtggcttc gggttccgca gccgacgagg
     1981 ccggtgttct gccgggggac atcatcacca ccctggaggg cctggtcctg gccaccgatg
     2041 ggtcgatggc cgactactgt gacatcctcc ggacgaaggg cgacgactcc actatgtcga
     2101 tcgaagtcct tcgctatcaa cagggggagt acttgaccgg gcaactgaat gggcgtccgc
     2161 tcgagacggc gggagtcttt ggacagcagc tggcgggtga cgtgccggac accggcagcg
     2221 ggtatttgga gtacacgcag gtaaccgacg actacggggc catcgtcgtt gaagttccaa
     2281 gcaactggat cgacatcgat ggcagcgcct gggtcgacgc gggcgacgtg ttcggagcct
     2341 cgatctcggc cgcgcccgat ctggatggtt tctttggcac ttgggataca cctggagtgt
     2401 tcttcgccgc ctccgatgat ctagcacgat ggggcggtta cgttcagctg ctggacttct
     2461 accggcccga cttcgccgcg gtctgcgagc tggacggccg ctacgactat gacgatgggt
     2521 actatcgggg caaatacgac tacttcgtga agtgtggtgg atcgggtggg gccgacttcc
     2581 tgatcctgac ggctgtgccc atagacacga gtgtgaagct gctgatcctg gtcgaggtcc
     2641 aaatcttgac caacgacgat gtcgatgcag cggatcgcat tctggcgact tttgacgtcg
     2701 tcggagccct cccataggca gaggagcgat ggacaaaggg cgccccgggc ggggcgccct
     2761 ttttgattct tagagcccgg tgagatttct ccctgctaga ccctcgatcg atgaccctgg
     2821 ctccatgagc tgttcgttga cacccgggcg ggcggcgtga tatcattcgg ccgctcgggt
     2881 tcggtgatta tgccggttgc ctgggcccgg tttcatctga catcattctt ctttgttctt
     2941 tgcaggtcgc agcttaggtc ggggctgtaa cgaaagaggc tccaccttcc aaatcgctga
     3001 agaaggatga agccacctga agaggtggat gtcgaaaccg gacattcacc tcttttattt
     3061 tgtgtgagac aaaggataac tggatgagtg gagaatttgt tctacggata atcggtctgc
     3121 tcgtactggc gattgccgga gtcatcgtgg gtgtccggct aagcatcccg gctggcggtc
     3181 cagcggagct gtgggcaggc gtactgggag cggtcggagc gctgttgagg ctggtcgcta
     3241 cgccgttcgt cactacccgg ccgctgcggg cgatccgggc aggggttgct caggtttcgg
     3301 ctcagactat ggtggccggc ctgagcggcc tgatcgtggg tctgctggcg gccgggctgg
     3361 tggcgttccc gatctcgctg ctgccgaccc ccttcggcca gctgctgcct ttcggcgccg
     3421 ccatcgtgtt cagctacctt ggagtcaccg tattcatcat gcgccagaac gatatcgcaa
     3481 acgtctggcg aggccggttt caaagttccg gcgatcaggg cggcgggggc ccgggtcgca
     3541 gtattctgct ggacaccagt gtgatcatcg atggccgaat cgccgacatc gcccggaccg
     3601 gcttcatcca ggggccgatg ctggtcccct ccttcgtcct gattgaactc caacatatcg
     3661 ccgactcggg cgacccgctg cgcaggcagc gcggccgccg cgggttggat attctcaacc
     3721 ggctgcagaa ggacaacccg atcccgctgc ggatcacgga tctggacgtg gagggggtgc
     3781 gggaagtcga tgacaagctg gtcatcttgg ccaagcagct gcgcagcccg atcctcacca
     3841 acgattacaa cctgaatcgg gtggcggagc tgcagggggt gacggtgctc aacatcaatg
     3901 agctggcgaa cgcggtcaag gcggtctatc tccccgggga gaagatcgaa gtccaagtta
     3961 tccaagaggg taaggagttc gggcaggggg ttggctatct ggacgatggg acgatggtcg
     4021 tcgtggaaga gggcaagagc aatatcgacc acacgatcac ggtgactgtg accaaagtgc
     4081 tgcagaccgc cgccgggcga atgatctttg cccggcccga cagccaatag gcggcggaga
     4141 tggggctgag gattttcaat accttgtccc gcaagaaaga gcccttcgag accgtggtcg
     4201 aagggcaggt gcggatgtac gtttgcggtc cgacggtcta cgaccaagcc cacatcggcc
     4261 atgcgatgtc ctccattgtc ttcgatgtaa tccgccgcta tctagagtac cgcgggtatc
     4321 aggtccgaca cgttatgaat tacaccgacg tggacgataa gatcatcgcc cggtccaagg
     4381 agctcggaca agacccgatc cagctggcac aacattacgt tgagcagtac gaccggcact
     4441 tgcaggagct caacctcctg ccgccggacg tgcggccacg ggtttcagat gaaatcgatg
     4501 ggattgttgg catggtcaaa gagctgatcg aggccgggca tgcctatcgg gtaaacgggg
     4561 acgtgtactt cgatgttggg agtgatccgc actacggcaa actttccggg cggaaactgg
     4621 atgagatgca ggcgggagcc cggctggaga tcgatgagcg caaacgagat ccggccgatt
     4681 ttgcactgtg gaaaacagct aagccgggcg agccgagctg ggaaagtccc tggggccggg
     4741 ggcggccggg atggcacata gagtgctcgg ccatgagcct gcatcacctt ggcgatcaaa
     4801 tcgatatcca cggaggcggg aacgacctga tatttccgca ccatgaaaac gagatcgccc
     4861 aaactgagag tctcaccggc cagcagttct cccgctactg ggtgcacaac ggaatgatgc
     4921 agctcaacgg ggagaccatg tcgaagtcca ccgggaacat gctgccgatt gcggaatttc
     4981 tgcagcagag cgaagccgat gtgctccgtc tgattgtgct caactcacac taccgcaccc
     5041 cgctggcgtt caactcggag atcgccgaag ccgcggcgcg gggattggac aggctgagga
     5101 ccgcaatgaa gccggaccag gccacaggcg acggaggcct caccggagag ggcgatcctg
     5161 ggagctccga agtcgagaag ttgctcgccc aggcagttga acgagcgcag caggacttcc
     5221 gggtctcaat ggatgacgat ttcaacaccc cccaggctct gagcagcttg tatgagctgg
     5281 tccgctcggt caaccaggcc cgggacgcgg gggtcagcca ggccgccctg gtggcagccc
     5341 aggaaatgct aggcgaactt ggcggggtac tgggattgag gcttgccagc gggaccacgc
     5401 cggatcccaa gctggccgcg ttggttgaga tgctggtgga gatacgggac gaactccgag
     5461 ccgccgagca gtgggagctc gcggatcgca tccggcagcg cctggcggac caagatattc
     5521 tgctggaaga tggcaagcat ggcacgagtt ggcggcaggg ctaaaccccg cccgcgcact
     5581 ttttccgggg acttgagtga gacgatctgg ggacgccacc cggtggaaga ggtgttgcgc
     5641 gccggccgga gaaaggttca ccgccttttt gttctccaag accacccacc gcccgagcga
     5701 gtgcttcggc ttgccaagga agccggtgtg aggctgcagc cggtcgaatt gttgccggtt
     5761 acctcgcaac aacatcaggg gatcgcggcc caggtggatc cctaccctta cgccgagctg
     5821 aatagcgtac tggagcgtgc ggcccgtgcc ggagaggcac ctctgctcct gctattggac
     5881 caggtccagg acccccaaaa cctcgggacg ctgctccgga cggccgaggc ggtgggtgta
     5941 cacggggtcg tgctgccgct caacggcgcc gctggcgtga ccccggcggt cgtggcggcc
     6001 tccgccgggg cctgtgaaca cctgtggatc gcgagagcga acctggccca agcgattcgg
     6061 cggttgaagc aggagggcct ctggatcgcg ggcttggaga acagtgagca ggccaaacgg
     6121 attgatcaga tcgacctgcg gccgccgctg gccctggttg tcgggagcga aggccgcggg
     6181 ctgaggcggc tggtagggga gtcctgcgat tttctggtcc agatccccat gcgcgggctg
     6241 gtcgaatcgc tcaatgcagc cgtggccgga tcggtggttc tctatgaggt ttggcggcgg
     6301 gcgggctatt ccggggcctc cggcggggga gttccccaat cggatcggca tgcgccagag
     6361 ccgagccggt cgagcaagaa tgctaacgat tgacgctccc tgcggttgct gatagactcg
     6421 gcgatcgcgg ttttggtata atcaccaagc gccacggtag gcgcgttttt gtcgtagggc
     6481 cgacgtagct caacggcaga gcaaccgcct tgtaagcggt aggttacgg
//
LOCUS       DATDPQ010000312        11612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2520159, whole genome shotgun sequence.
ACCESSION   DATDPQ010000312 DATDPQ010000000
VERSION     DATDPQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520159"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VI520_06980"
     CDS             <1..309
                     /locus_tag="VI520_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HLF37660.1"
                     /translation="LTGHPTERLPNHASFVFEGVDSNQLLAALDLAGYACSSASACKT
                     GEPEPSEVLLAIGLDPRLATSSLRVTVGRQTTSVEVEAFLTSLPEAVSRLRAIEPAFP
                     "
     gene            306..1376
                     /gene="mnmA"
                     /locus_tag="VI520_06985"
     CDS             306..1376
                     /gene="mnmA"
                     /locus_tag="VI520_06985"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HLF37661.1"
                     /translation="MNSRRTIAVAMSGGVDSSMAAALLVEQGEQVFGLMLRLWDAHPD
                     RPNRCCSPRDMANARMVAAQLEIPFYVLDVREPFRQAVVEPFIAGYLEGITPNPCMAC
                     NRQIRWGHLLRHALQLGATHLATGHYARIEGSDSQPRLYRSRDRMKDQSYVLSVLRQA
                     DLSRSRFPLGELTKDQVREAATQLRLPVADRPDSQDLCFLGGADYREFLRDSVGALWG
                     PGPILDQAGTPLGTHQGLPAYTIGQRKGIGVSAPVPFYVVAKDKERNALVVGPRAALA
                     RSSFRVDRLQWVAGEPPELGTSLTVQVRYRSSAVPVRLQLNGTTASAEVELEQPVLQI
                     TPGQAAVFYSGEECLGGGVIIQ"
     gene            1373..1678
                     /locus_tag="VI520_06990"
     CDS             1373..1678
                     /locus_tag="VI520_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37662.1"
                     /translation="MTLPGLYFALLISTGLGLVFHFVRGGGLARLALYLVTAWVAFLA
                     GHLVAELLDWHLFRVGPINLFAAVLAALLGLLTASLLAGPERGRRAAGRDRKAGPPG"
     gene            1700..2122
                     /locus_tag="VI520_06995"
     CDS             1700..2122
                     /locus_tag="VI520_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37663.1"
                     /translation="MALTESASTPSSRRKQTLYIAGGGLVLLALIAGLVLAVSAMVRN
                     PQGTETIRDIVIIFVAAETLLIGVALILLLVQLARLTALLQNEIRPLLESTNETVATL
                     RGTSEFLSQKMVAPVIKANSSVAAVRRAIGLFRAGRAK"
     gene            2146..2481
                     /locus_tag="VI520_07000"
     CDS             2146..2481
                     /locus_tag="VI520_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HLF37664.1"
                     /translation="MADRDDFGAFFSGFLIGGIVGAAVALLLAPQSGEQTRTLIREKG
                     IELRDQVELTASDARAKAERIAEEARSRAADLQQRGQVVLEEQKTRIEQAIERGKKAA
                     QPKRAEVES"
     gene            complement(2551..4164)
                     /locus_tag="VI520_07005"
     CDS             complement(2551..4164)
                     /locus_tag="VI520_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HLF37665.1"
                     /translation="MPKVVSVEQMRQIERAADSAGHSYAQMMEHAGWSVAQVIVEQFA
                     PVEGKQVLVLAGSGNNGGDGLVAAEHLAQAGMHVSVYLTKQRAADDPHLGKLRDRGTL
                     IAIADQDQRSRVLRLQVGRAQLIVDAILGTGFELPLQGAAQEVLKVVQNSLGQRADRP
                     VIVAVDCPSGLDCDSGAVAEETIPADLTVTLAAAKPGLLRFPGAGFTGRLVVADIGLP
                     SDQAQLANIEVELASPIEVRPLLPGRPRDAHKGTFGRALIIAGSANYPGAALLAARSA
                     YLVGAGLVSLAVPAPLQPMIAGELPEATWILLPEKSGAITPDALGQLEPEWTRSQAVL
                     VGPGLGLESATGAFIAELFGRRLKSEERSGASAQALPGRSLPPCVVDADGLKHLSALA
                     GWPEAVPEGTILTPHPGEMSLLTGSSKEAIQAERLDAARRSAREWGHIVVLKGAHTVI
                     AEPAGRATILPFATPALARAGTGDVLAGVIVGLRAQGVPAFSAAVLGGYLHGRAGELA
                     AATLGTTASVLASDVAAAIPQALAELQAS"
     gene            4197..5435
                     /locus_tag="VI520_07010"
     CDS             4197..5435
                     /locus_tag="VI520_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HLF37666.1"
                     /translation="MRERRVCVNAAWRGLTAGAAPGYTAASLLPMITRERLEALEDQA
                     LAPFAHRSRDSRGRVYPEPEPTYRTAFQRDRDRILHTTAFRRLEYKTQVFVITEGDYY
                     RTRLTHTLEVAQIGRSLARALGANEDLVEAISLAHDLGHPPFGHSGEATLSRLMAAHG
                     GFDHNRQSLRIVTELERRYPDFPGLNLTWEVREGIVKHETEYDVADAAGYEPQKRGHL
                     EAQISNLADELAFTAHDLDDGLRSELISPDLLEGVALWGLLRQSIGWLGGQLDDLTRH
                     RLIRRLIGLEVSGLLEATSARIEAAKVRSVEDLQNLDYNLVRYSDDFVVLNQQLKDFL
                     YSNMYQHFRVVRMQSKAERIVEDLFKSYVEKPEILPPETQARARQFELYRPICDYLAG
                     MTDRFAIQEHRKLFEPETLP"
     gene            5442..5909
                     /gene="greA"
                     /locus_tag="VI520_07015"
     CDS             5442..5909
                     /gene="greA"
                     /locus_tag="VI520_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HLF37667.1"
                     /translation="MADDQSFLTAEGAAKLRKELDELKGPRRSEMAERLRRAVRQGDL
                     SENADYISAKEQQAFLEGRILELETVLRNATVVERTAPSEQVEVGSTVVVQEQGETKT
                     TFQIVGAKEADPRAGKISHVSPIGKALLGKKVGESAQATTPGGPVRFKVLEIR"
     gene            complement(5906..6919)
                     /locus_tag="VI520_07020"
     CDS             complement(5906..6919)
                     /locus_tag="VI520_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37668.1"
                     /translation="MEAIAGRKWLLPALALIFGLAAGLGYAWGLNPVQWINGEPQQLR
                     ADLQEDYLRMAIDSYSVNRDSDLALQRYSALGESAKLTLDAVAVDPQELSPTAIQGFR
                     ALIEIDQEASATPQAGESEEPSSPLPSLSKLILPVCGGTLFLGLLLVTVLVLRSRSRE
                     NSAAPNPPSATEEAEVGPAVQPFASQPEFAGDDSLATFRTIYNLGEDKYDDSFSIESP
                     TGDFLGECGVGIGDLIGVGEPNKVSAFEVWLFDKNDIQTVTKVLMSNYSYHDDETRTR
                     LSAKGDPVVAEPGGVISLQTASLEVEARIVDMIYGESALPSQSFFERLTIELRAWSRD
                     GAG"
     gene            complement(7018..9330)
                     /gene="topA"
                     /locus_tag="VI520_07025"
     CDS             complement(7018..9330)
                     /gene="topA"
                     /locus_tag="VI520_07025"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HLF37669.1"
                     /translation="MEAYCVKCKQKREILNPEPTFTASGSPASRGTCPVCGSKLYRMG
                     RTEAHAGLEPPPARPRSTNSRHGKLVIVESPAKARTVGRYLGKGYQLKASVGHVRDLL
                     RSQLSVDVENDFEPKYRVPNEKRIVVKELKKAAAKASEVYLATDPDREGEAIAWHLLE
                     AAEISPDQAKRVVFHEITEEAIHQAFNEPRAIDLDLVDAQQARRILDRLVGYNLSPIL
                     WAKVRGRLSAGRVQSAALRIVVDREREIEEFQAREYWTLDADFLHPDKPPGFRARLVR
                     VDAEEPGLASESMVEPLLADLRGAEFKVAEVQPGTRTRRPLPPFTTSTLQQSAARRLR
                     FSARKTMVVAQQLYEGIDLNEHEPTGLITYMRTDSSQVASSAQAEARAVVTSSFGKEY
                     LPERPPIYRAKSRTAQEAHEAVRPTSSKRTPESIRSFLSSEQYKLYELIWNRFIASQM
                     KPAVYETLTIEVEGVSADHAYQFRVNASSLRFAGFLRVHPDWEGGELDGEISLEKLPQ
                     LEKDDPLRLEGLFPEQHFTEPPPRFNDASLIKALEEYGIGRPSTYAPILSTLEQRGYV
                     RREKRRMIPTEIGEIVNDLLVEHFPEIVDLGFTARMEEELDKVAAGERNWVELLRDFY
                     GPFEQQLAMAQKHMPEIKAEPEPLGRACPDCGNQLLIRHGRFGKFIGCSNFPECRHTE
                     PWLEKIGVRCPLDGGELVERRTRKGRIFYGCSNYPACEFTSWKRPDPTPCPNCGGLLV
                     HETRKSVRCLQCERQFDPEGLPSMLPDVAR"
     gene            complement(9345..10448)
                     /locus_tag="VI520_07030"
     CDS             complement(9345..10448)
                     /locus_tag="VI520_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLF37670.1"
                     /translation="MSFERTYPVIMAGGGGTRLWPLSRADRPKQSLPLLEGRTLFQLA
                     VRRLLPAFSLDRIRVVTIQPLVQQLVEQVPELSEQNFLIEPSPRGTASVIGLAATVLR
                     RKDPQAIMACLTADHLIGRPDRFLQLLAAAETVAQRGELVTLGISPTHPATGYGYIHV
                     GESTGSVASFPISRVLQFKEKPDRSQAEHYLETGEYYWNSGMFVWTVGRILEQIEQWM
                     PELSKGLEQISSALESNAGQATIEQVWSGLRSQTIDYGIMERAQGVSVIGADDLGWSD
                     VGGWDRFSELLSTDSDGNQIFAEHALMVETQDSLIYQERPDHANRLIAAFGLRDLIVV
                     DTPDVLLVCRRERAEEVRQLVEKLAAAGRTEYL"
     gene            complement(10445..>11612)
                     /gene="dprA"
                     /locus_tag="VI520_07035"
     CDS             complement(10445..>11612)
                     /gene="dprA"
                     /locus_tag="VI520_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLF37671.1"
                     /translation="LSENRYGMLRRLCAELIMTSGDVKYWIGFNLVSGIGPVRVRSLV
                     QTFGSIEAAWNAPVEELRRVGLGEKLVQRLIEARSALDLTKELGRVQQLGLQVLPMDD
                     PQYPARLKEIESPPLVLYLSGQLVPQDEWAAAIVGTRRATPYGKAVAAELAGGLAASG
                     VTVVSGLARGIDGIAHQAALEAGGRTLAVLGSGLDHIYPPEHRNLAHSIASSGAVLSD
                     YPLGTSPEGGNFPARNRIIAGLSLAVIVVEAGESSGALITADFAAEQGRDVFAVPGRI
                     YDRASLGTNRLIAAGAFPVTNVDDVLEVLNLESITAAAPREPSLPKDATERRVLEALS
                     GDPVHIDELRERCELPIAELTACLSMLELRGQARQVGGMHYVRAHEPRAAYRVG"
BASE COUNT         2165 a         3696 c         3547 g         2204 t
ORIGIN      
        1 ctgaccggcc atccgaccga gcgcctcccg aaccacgctt cgtttgtttt cgagggcgtc
       61 gacagcaatc agctcctggc cgccctggac ctggctggat acgcctgctc atcggcctcc
      121 gcctgcaaga ccggtgagcc ggagccctcc gaagtgctgc tcgccattgg actcgaccct
      181 cggctcgcaa ccagttccct tcgagtcacc gtcggacggc agaccacatc cgtggaggtg
      241 gaggcctttc tgaccagcct gccggaagca gtctcccgat tgcgggcaat cgaaccggcg
      301 tttccatgaa ttcgcggcgc acgatcgccg tcgcaatgag cggcggcgta gacagttcga
      361 tggctgccgc gctgctggtt gagcagggtg agcaggtgtt cggtcttatg ctgcgcctgt
      421 gggatgcgca cccggaccgc cccaatcgat gctgctcgcc ccgcgatatg gccaacgcac
      481 gaatggtcgc cgcgcagttg gagatcccct tctacgtatt ggacgtgcgg gaacctttcc
      541 gccaggccgt ggttgagccg ttcattgcgg gatacctgga ggggatcacc cccaacccgt
      601 gcatggcctg caatcgacag atccgatggg ggcacctcct acggcatgct ctccaacttg
      661 gcgcaaccca cctggccacc ggacactatg cccggatcga gggatcggat agtcaaccgc
      721 gcctctaccg ctcgcgggat cgaatgaaag atcagtctta cgtgctcagc gttctgagac
      781 aggcagatct cagtcggtct cgcttcccgc ttggcgaact cacgaaagac caggttcgag
      841 aggctgcaac ccagctccgc ttgccggtcg cagatcgtcc ggatagtcaa gacctctgct
      901 tcttgggcgg cgccgattac cgtgagtttc tgagggacag cgtgggggcc ctctggggac
      961 ctgggccgat cctagaccaa gcaggaacgc cgctcggcac ccatcaaggg ctgcctgcgt
     1021 acacgatcgg ccagcggaag ggaatcgggg tctcggcccc cgtgccgttc tacgtggtcg
     1081 cgaaagataa agaacgcaat gcgctcgttg tcggcccgcg ggcggcgctc gcccggagtt
     1141 cctttcgggt ggaccgccta cagtgggtgg ccggcgaacc tcccgaattg ggaacttcgc
     1201 tcacggtgca ggtccgatat cggtcttccg cagtcccggt tcggctgcaa ctcaatggca
     1261 cgacagctag tgcggaggtc gagctggagc agccggtgct ccagatcacc ccaggtcaag
     1321 ccgcggtctt ctactctggg gaggagtgcc tcggcggcgg ggtcatcatc cagtgaccct
     1381 tccgggcctc tactttgccc tgctgatttc caccggcctc ggacttgtct ttcatttcgt
     1441 ccgaggcggc gggcttgctc ggttggcgct ctacctggta accgcctggg tggcattcct
     1501 cgccgggcac ctggtggcgg agctgctcga ttggcatctc ttcagagtcg gcccgatcaa
     1561 tctgttcgcg gcggtgctgg ctgcgctgct tgggttgcta accgcttcat tgttggccgg
     1621 gccggagcgc ggccgccgtg cggctggacg ggatcggaag gcgggtccgc caggctaatg
     1681 tgctacaata tataattcga tggcgctgac cgaatcggcg agtaccccct ccagccggcg
     1741 caagcaaact ctctacatcg ccgggggtgg gttggtattg ttggccctga tcgcggggtt
     1801 ggtgttggcc gtcagcgcga tggtgcgcaa ccctcagggt acggagacga tccgagatat
     1861 cgtgattatc ttcgtggcag cggaaaccct tttgatcggg gtggcactga tcttgctgtt
     1921 ggtccaactc gcgcggctga ccgccctgct gcagaatgag atacggccgc tgcttgaatc
     1981 gacaaacgag acggtggcga ccctgcgagg tacaagcgaa ttcttgagcc agaagatggt
     2041 cgcaccagtg atcaaagcca acagttctgt ggccgcggtc cgcagagcca ttgggctttt
     2101 tcgggccggc cgcgccaagt agaatcggaa acggagggat gacggatggc agatcgagat
     2161 gatttcggag ccttcttctc aggcttcctg attggcggaa tcgttggggc cgcggtcgct
     2221 ttgctgcttg cccctcaatc cggcgaacag acccggaccc taattcgcga gaaagggatc
     2281 gagctgcgcg accaagttga attgactgcc tcggatgcac gggcgaaggc cgagcggatc
     2341 gccgaggagg cccgctctcg ggcggcagac ttgcagcagc ggggccaggt cgtccttgag
     2401 gagcagaaga cccgaatcga gcaggcgatc gagcggggca agaaagccgc tcagcctaag
     2461 cgagccgagg tcgagagcta gtccctaagc tgcttggatt tctcgaacaa gcgaacagcc
     2521 ccgtcgagcc ggggctgttt ctttttgggg tcagcttgcc tggagctcgg ccagcgcctg
     2581 cgggatcgca gccgcgacgt cgcttgccaa taccgaagcc gtggttccca gagtcgcggc
     2641 ggcgagctct cccgcccggc cgtgaagata accgcccagg actgccgctg aaaaggccgg
     2701 cacaccctgg gcgcgcagcc cgacgatgac tcctgccagc acgtcgccag tcccggcccg
     2761 ggccagcgcc ggtgttgcaa aaggcaggat cgtggcccgg ccggccggct cagcgattac
     2821 cgtgtgggcg cctttcaaca ccacgatgtg cccccactcg cgggccgacc gtcgggctgc
     2881 atccaaacgc tcggcctgga tcgcctcttt cgagctgccg gtgagcaggc tcatttcgcc
     2941 cgggtgaggc gtcaggatcg tgccctccgg gacggcctcc ggccaaccgg cgagagcgct
     3001 caggtgcttg agcccatccg cgtccacgac gcacggcggc agcgagcggc cgggcaatgc
     3061 ctgtgcgctg gcgcccgacc gttcttcaga tttcaaccgt cgtccaaaaa gctcggcgat
     3121 gaaggcgccg gttgccgact cgagccccag cccgggcccg actagtaccg cttggctcct
     3181 cgtccactcc ggctcgagct gtcctaacgc gtccggagtg atcgccccgg acttctcggg
     3241 aagcaggatc caggtggctt cgggcagctc cccggcaatc atcggttgca ggggcgcggg
     3301 caccgccaag ctgacgagcc ccgccccaac cagataagcc gatcgggcgg ccagcagggc
     3361 tgcgccgggg taattggccg atccggcgat aatgagcgcc cggccaaagg ttcctttgtg
     3421 cgcatcgcgt ggccgtcccg ggagcagcgg tcgaacttca atcggactgg ccagctcaac
     3481 ctcgatgttt gccaactgag cctgatccga tggcaggcca atatctgcga ccaccagccg
     3541 cccagtgaag ccggcccccg ggaagcgaag cagtccgggc ttggctgccg caagcgtgac
     3601 tgtgagatcg gcggggatgg tctcctcggc aaccgctccc gaatcgcagt cgagtccgga
     3661 aggacagtcc accgctacga tcacggggcg gtcggcgcgc tggccgagcg agttttgaac
     3721 gaccttcaag acctcctgcg cggctccctg cagcggcagc tcgaagccag tcccgaggat
     3781 cgcatccacg atgagctggg cccgcccgac ttgcaggcgg agaactcgcg agcgctggtc
     3841 ttgatctgcg atcgcgatca gggtcccgcg gtctcgaagt ttccccaggt gcgggtcatc
     3901 tgctgcccgc tgcttggtga gatagaccga tacgtgcatt cccgcctgag ccaggtgttc
     3961 ggcagccacc aagccgtctc cgccattatt gccggagccg gccaggacca gaacctgctt
     4021 gccttcgacc ggggcgaact gctcgacgat cacctgtgcg accgaccaac cggcgtgctc
     4081 catcatctga gcgtagctgt gcccagccga atcggcagcc cgctcgattt ggcgcatttg
     4141 ctccacgctg actaccttcg gcaccctcga cctccgacgt cccggccccg cggattatgc
     4201 gcgaacgccg ggtgtgtgtc aacgcagcct ggcgcggatt gacagccggc gcggcccccg
     4261 gttacactgc cgcttcgctg ctcccgatga tcacccgtga acggctcgaa gcgctcgaag
     4321 atcaggcatt ggcgccgttc gcccatcgta gccgagattc ccgcggccga gtctatccgg
     4381 aaccggaacc cacctaccgc accgcgttcc agcgcgatcg agatcggatc ctgcacacca
     4441 ccgcgttccg gaggctggag tacaagaccc aggtgttcgt gattaccgag ggcgactact
     4501 atcggacccg actcacccac acccttgagg tcgcacagat cggacgcagc ctcgctcggg
     4561 ccctcggcgc caatgaggat ctggtggagg cgatctcact ggcccacgac ctgggtcacc
     4621 cacccttcgg gcactccggc gaagcaaccc tcagccggct gatggcggcc cacggtgggt
     4681 tcgaccacaa ccgccaatcg ctgaggatcg ttaccgaatt ggagcggcgc tatccagact
     4741 ttcctgggct caacctaacc tgggaagtcc gcgaaggaat cgtcaagcat gagaccgaat
     4801 acgatgtcgc ggatgccgcc ggctacgagc cccaaaaacg cgggcacctg gaagcccaga
     4861 tttccaacct ggcggatgaa ctggccttca ccgcccacga tctggacgac ggcttgcgct
     4921 cagagctcat ctcgccggac ctgctcgagg gcgtggccct ctggggtttg ctgcggcaaa
     4981 gcatcgggtg gctcggcggc cagctcgatg atctgacccg ccatcggctg atccggcggc
     5041 tgatcggttt ggaggtctcc ggactgctgg aggcgaccag tgcccgcatc gaagccgcca
     5101 aggtccggtc ggtggaagac ctgcagaact tggactataa cctggtcaga tactcagacg
     5161 actttgtggt tctcaaccag cagctgaagg attttcttta ctcgaacatg taccaacact
     5221 ttcgggtggt gcgaatgcag agcaaggcgg agcggattgt ggaagacctg ttcaagtcct
     5281 acgttgagaa acctgaaatc ctcccgcccg agactcaggc cagagcccgc caattcgagc
     5341 tctaccgtcc catatgcgac tacctggcgg gaatgaccga ccggttcgcg atccaggagc
     5401 atcgcaagct attcgagcct gagaccctcc cttagttggc catggcagat gatcaatcct
     5461 ttctgaccgc cgaaggcgct gccaagctcc gcaaagaact cgacgagttg aagggacccc
     5521 ggcggtcgga gatggccgag cggctccgga gggcggtgcg ccaaggcgat ctgagcgaaa
     5581 acgccgacta cattagcgcc aaagagcagc aggcgtttct cgaggggagg atcttggaat
     5641 tagagacagt gctgcgcaac gccacagtcg tcgagcggac cgcgccatcc gaacaggtgg
     5701 aggttggcag caccgtagtt gtacaggaac agggcgaaac caagacgacc ttccagattg
     5761 tgggagccaa agaggccgat ccgcgggccg gcaagatctc tcatgtatcg ccgattggta
     5821 aggctctgct ggggaaaaag gttggagagt cggcccaagc cacgaccccg ggcgggccgg
     5881 ttcgcttcaa ggtcctcgag attcgttaac cagccccgtc gcgcgaccac gccctgagct
     5941 cgatcgtcag ccgctcgaag aagctttggg aggggagtgc gctctccccg taaatcatat
     6001 ccacgatcct ggcttccact tccagtgatg cggtctgcaa ggagatcacc cctccgggct
     6061 cagcgacaac cggatctccc tttgcagaca aacgggtgcg cgtttcgtca tcatgatagg
     6121 aatagttact catcagcact ttggttacag tctgaatatc gttcttgtcg aacaaccaga
     6181 cttcaaatgc ggagacctta ttcggctccc cgaccccaat caggtctcca attccaactc
     6241 cgcattcccc aaggaagtcc ccggttgggc tctcgatgct gaatgaatcg tcatacttgt
     6301 cttccccgag gttgtagatg gtgcgaaagg tcgccaggct atcgtcgccc gcgaattctg
     6361 gttgggaagc aaatggctgg accgccggcc cgacttccgc ttcctcggtg gcggacggcg
     6421 ggttgggcgc tgctgagttt tcccggctgc gggagcgaag gaccaggacg gtgaccaaca
     6481 gcaatcccag gaaaagggta ccgccgcaca ccggtaggat gagtttggat agtgagggga
     6541 gcgggctact cggctcctcg gattctccgg cctggggggt ggcgctggcc tcctggtcga
     6601 tttcgatcag ggcccggaag ccttggatag cggtcgggct gagctcttga ggatcgaccg
     6661 cgacggcatc cagagtgagt ttggccgatt cgccgagggc gctgtatcgt tggagggcca
     6721 gatcagagtc tcgatttact gaatacgagt cgatggccat ccgaagatag tcttcttgca
     6781 gatcggcccg caactgttgt ggctcgccat taatccactg gacagggttt agaccccatg
     6841 catagcccag accggctgcc aggccgaaga tcagggccag ggcgggcagc agccatttgc
     6901 gaccggcgat cgcttccatc atttccctct tcctcaatcc ctccggcctc gagcttgctt
     6961 acagcagggg ccggattcta gactgaacga gaagtgagag caaccgaaga gttgcactta
     7021 gcgtgccacg tccggcagca tagagggaag cccctcagga tcgaattggc gctcgcactg
     7081 taggcagcgc accgactttc gggtctcgtg caccagcagc ccgccgcagt taggacatgg
     7141 ggttgggtca ggtctcttcc aggaggtgaa ctcgcaggcc ggataattcg aacacccata
     7201 gaagatccgc cccttgcggg tacgcctttc gaccagctcg cctccgtcca gcgggcaacg
     7261 aacgccaatc ttttccagcc acggttcagt gtgacgacac tccggaaagt tggagcagcc
     7321 gatgaacttc ccaaagcgac cgtgccgaat caggagttgg tttccacagt ccgggcaggc
     7381 ccgccctagt ggctccggct cggccttgat ttcgggcatg tgcttctggg ccatcgcaag
     7441 ctgttgttca aatgggccgt agaagtccct gagcagctcc acccaattcc gctccccagc
     7501 agctaccttg tccagctcct cttccatccg ggctgtgaat cccaggtcaa cgatctccgg
     7561 gaagtgttca accagcagat cgttaacgat ttccccgatc tcggtcggga tcatccggcg
     7621 cttctctctc cggacatagc cacgctgctc aagggtggag aggattgggg cgtaggtcga
     7681 gggccgtccg atcccgtact cctccagtgc cttgatcagg ctcgcgtcat tgaatcgcgg
     7741 gggcggctca gtgaagtgtt gttctggaaa gagtccttca agccgcagcg ggtcatcctt
     7801 ttcaagctgg ggaagcttct ccaggctgat ctcgccgtcg agctcgcctc cctcccagtc
     7861 gggatggacc cgcagaaaac cggcgaagcg caagctggag gcgttcactc ggaattgata
     7921 ggcgtgatcc gccgagactc cctcgacctc gatggtcaac gtttcgtaga cagcaggctt
     7981 catctgtgag gcgatgaatc ggttccagat gagctcatag agcttgtact gttcagagct
     8041 caagaagctt cgaatggatt ccggcgttcg tttcgaggag gtcggccgga ccgcctcatg
     8101 cgcctcctgg gcggtgcgag acttcgcccg gtagatcggc ggccgctcag gaaggtattc
     8161 cttgccgaaa ctggaagtga ccaccgcccg ggcttccgcc tgcgcagagc ttgccacttg
     8221 tgaggagtcc gtccgcatgt aggtaatcag gccggttggc tcgtgttcgt tcaggtcaat
     8281 cccttcatag agctgctggg ccacgaccat ggttttccgg gccgaaaacc ggagccgacg
     8341 agcggccgac tgctgcaagg tgctggtcgt gaacgggggc aacggccgcc gggttcgggt
     8401 accgggctgg acttcggcga ctttgaactc cgcgccccgg agatcggcca ggagcggctc
     8461 aaccatactc tcggaggcaa gcccaggctc ctcggcatcc acccggacca agcgggcccg
     8521 aaatccgggc ggcttgtccg gatgcaggaa atctgcatca agggtccagt actcgcgggc
     8581 ctgaaattcc tcgatttcac gctcccggtc cacaactatc cgcagggcag ccgattgcac
     8641 ccgtccggcc gacaatcggc ctcggacctt ggcccacagg atcgggctga ggttgtagcc
     8701 gaccaggcgg tctaggatcc gccgagcttg ctgggcatcg accagatcca ggtcaatggc
     8761 ccggggttcg ttgaaggcct ggtggatggc ttcctcggtg atctcgtgga aaaccacccg
     8821 cttggcttga tcagggctga tttccgcggc ttccagcagg tgccaagcga tggcttcacc
     8881 ttcccgatcc gggtcggtag ccaggtagac ctcactcgcc ttggcggctg ctttcttgag
     8941 ctccttgacg acgattcgct tttcattcgg cacccgatat ttgggctcga agtcattctc
     9001 gacatcgacc gagagctgcg aacgcagcaa atcccgaacg tggccgaccg aggccttcag
     9061 ttgataaccc ttacccaagt accggccgac cgttcgggcc ttcgcaggcg attccacaat
     9121 caccagcttc ccatgccgac tgttggtcga gcgcggccgg gccggaggcg gctcgaggcc
     9181 tgcgtgcgct tcggtgcgac ccatccgata cagcttcgac ccgcacaccg ggcaagttcc
     9241 gcggctcgcc ggagaaccgg aggccgtgaa ggtcggttcc gggttcagga tctcacgctt
     9301 ctgcttgcac tttacacagt aggcttccac gctgtggttt tcccttagag atactcggtc
     9361 cggccggcag ctgccagctt ctcgacgagc tgtcggacct cctcggcccg ctctcgtcgg
     9421 caaaccagca acacatccgg tgtatcgacc acgatcagat cgcgcagacc gaacgcggcg
     9481 atcagccgat tggcatgatc ggggcgctct tgatagatta aggagtcctg cgtctcgacc
     9541 atcagtgcat gctctgcaaa gatctggttt ccatcggagt cggttgagag cagctccgaa
     9601 aaccggtccc agccgccgac atcgctccac ccgagatcat ccgccccgat cacacttact
     9661 ccctgggcac gctccatgat tccgtagtca atcgtttggc tgcgcaggcc ggaccagact
     9721 tgctcgatcg tcgcctggcc tgcgttggat tctagggcgg aagaaatctg ctccagaccc
     9781 ttactgagct ccggcatcca ctgctcgatc tgctccagaa tccggcccac cgtccaaacg
     9841 aacatgcccg agttccagta gtattctccc gtctccaaat agtgctcggc ctggctccga
     9901 tcgggtttct ctttgaattg gagcacccgg ctgatcggga aacttgccac gcttccagtg
     9961 gattcaccaa catgaatata tccatatcca gtcgccggat gggtcggtga gattcccaag
    10021 gtcaccaact cacctcgctg ggcgaccgtc tccgccgcgg cgagcagctg cagaaaccga
    10081 tcgggccggc caatcagatg gtcagcagtg agacaggcca tgatcgcctg gggatccttc
    10141 cggcgcaaga cggttgccgc gaggccgatg accgaggccg tgcctcttgg ggatggttcg
    10201 atcagaaagt tctgctcgct gagctcgggg acctgttcaa ccagctgttg gaccagaggt
    10261 tgaattgtca ccactcggat ccggtccagc gagaacgccg gcaacagacg ccggactgcc
    10321 agctggaaca gggtccggcc ttctagcaga ggcagactct gtttcggtcg atcagctcgg
    10381 gataagggcc acaggcgggt tccaccaccg cctgccatga tcaccggata cgtccgctcg
    10441 aagctcatcc gactcgatag gccgccctcg gctcgtgggc gcgcacgtag tgcattcccc
    10501 cgacttgccg ggcctgcccg cgcaactcga gcatcgagag gcaggccgtc agctctgcga
    10561 ttggaagttc gcagcgttcc ctgagctcgt cgatatggac cggatcgccg gaaagcgctt
    10621 caagcaccct acgctcagtc gcatccttcg ggagcgatgg ctcacgcggg gcggcggcag
    10681 tgatcgattc cagattgagg acttccaaga catcatcgac attcgtgacc gggaatgcac
    10741 cggccgcaat caagcgattg gtccccaggc ttgcccggtc gtaaatccga cccggtaccg
    10801 caaaaacatc gcggccttgt tcggccgcga agtctgccgt gatcaaggct ccgctgctct
    10861 cccccgcttc gaccacgatt accgccaggg agaggccggc gatgattcga ttgcgggccg
    10921 ggaagttgcc tccctccgga ctggttccca ggggatagtc actcagcacc gccccagaac
    10981 tggcaatcga gtgagccagg ttgcggtgtt ccggtgggta aatgtggtcc aaacccgagc
    11041 cgagaaccgc tagcgtccga cctccagctt ccagcgcggc ttggtgggca attccgtcga
    11101 tcccgcgtgc cagacccgag accaccgtca cgccgctggc ggccaagccc ccagccaact
    11161 ccgcagcgac cgccttgccg tagggcgtcg cccgccgagt cccgacgatc gcggcagccc
    11221 actcgtcttg gggcaccagc tgccccgaca ggtagagaac cagcggcggc gactctattt
    11281 cctttagccg cgctgggtat tgcggatcgt ccatcggcag gacttgcaac cccaactgct
    11341 gcactcggcc gagctccttc gtaagatcaa gagccgatct cgcctcgatc agccgctgta
    11401 caagcttctc tccaaggccg accctgcgca gctcttccac cggcgcgttc caagcggcct
    11461 cgatactgcc aaaggtctga acaagcgagc gaactcggac cgggccgatt ccggagacca
    11521 ggttgaaccc aatccagtat ttgacgtcgc ccgaagtcat aataagctcg gcgcagagcc
    11581 ggcggagcat accatagcga ttttctgaaa gt
//
LOCUS       DATDPQ010000313         4355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_947464, whole genome shotgun sequence.
ACCESSION   DATDPQ010000313 DATDPQ010000000
VERSION     DATDPQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947464"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..594
                     /locus_tag="VI520_07040"
     CDS             319..594
                     /locus_tag="VI520_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37672.1"
                     /translation="MTLEQVVHRIATDSAFAASVREYPEAALKGEAPELSSSEIRSLM
                     AALTADETQKAPGAALPWFESQLASNPDNRPQTALPWFEAQLGMRPV"
     gene            672..1535
                     /locus_tag="VI520_07045"
     CDS             672..1535
                     /locus_tag="VI520_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLF37673.1"
                     /translation="MRQSLLAGEGQPQAASPRRSWLSLPGLCCRAAGGNPQDADGVAA
                     AWVLLYTAAHVLDSIQDRDPPERWWAELGEGPAINVATGLLSTAWVVLDRIEADRSSV
                     RRVIEDFHSTVLWMASGQHLDLTLSQLTPDKAWAIAEAKSGAFFALACRSGARMAGAA
                     EATAEQYGRYGLNLGLIVQIGDDMEDLVRSQGFPPLAVAYCLQTASPKARGRLSTIMQ
                     ASSAGPSAQVEAARILTASGALLYLGTKLLQFQSFALESLDASGAEPTASKELTALVR
                     ALAPSVEPRPT"
     gene            1538..3931
                     /locus_tag="VI520_07050"
     CDS             1538..3931
                     /locus_tag="VI520_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37674.1"
                     /translation="MISLAFHRLRYAFPFLLAIGSLALIFWATDFQLHRGYEGFDWTP
                     LSGAVLDVDPLAPASESLRVGDRIVSVEGVPAILAPQIHANIGPGDTVHYLIERDRSQ
                     FAVDLRMADLPPRVVFQSLVPLAIAATFWAIGLAVLSFGQAGAEARPFFRFCLIASGV
                     LGAGSLSTAGPIWSSTLFNILLWFAGPIAVDLHLRFPEARRHSRAMSAVLYGGAILGS
                     LPFLLLGATGVRASAWSQLVFTSGRLFLSGNLLLVVWLLLSSYRRATSVQARQRIRLV
                     ALGGGAGMLLVIVLSILPGALTDRPLVPYEVSFAFLIALPASYGYAIARHRLIRLERF
                     LSRGAAYTLVFAILAAIYLGVTTALDRLFSDELLEQPLINMLVILLLAATAVVIYRRI
                     QALVDFAFYGGWYDYRSAVARITEGLEEQKSSEELAATLSTRLQATLRLERACVFVAT
                     RDGSLAANGCELPDEAAAPASPLALPAQGQVVQYLKLNPRPVSAEALRQSLLDRPLTR
                     DERGTLLATTDCLMVPVAGSDGLLAVMALGRRRGGEGFSAEDLDILDLVARHAGAAIE
                     NVRLTGEVRQHVVEVEQLHKRLLRAREEERKSLARELHDEAIQSLVGMNYRLAHLESG
                     QTQTLRQEVLRIVEELRGMIHRLRPPALDNFGLVTAVRSELRERSVRSHGSRRIDLHV
                     EGDPELGISEEVGLCLYRVMQEALNNIERHSEAAQVDVALVMGPEEIRLSVRDNGKGF
                     PVPQQLGLLLEADHFGLVGVRERLELLHGTLEIDSAPGSGTTLRARIPLESNSSGPA"
     gene            3943..>4355
                     /locus_tag="VI520_07055"
     CDS             3943..>4355
                     /locus_tag="VI520_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37675.1"
                     /translation="MENSDGKHADKPIRVLVADDHPVVRSGIRDELAKHPDLLLLGEA
                     VDGDQALELSRTLRPDVLLLDINMPGLQPVKVVRELAKLNGGPQVLVLSAYSDVEYVF
                     EMLKAGVHGYLLKDEEPGRIAEGIRAVAAGKTWLSS"
BASE COUNT          762 a         1357 c         1415 g          821 t
ORIGIN      
        1 cttctcgttg catcaattcc tcgtcagcag gatttggtca aagagaacac ggggcccgga
       61 ttcgcggctg aaaccgcgcg acccggcttt ccccctgctg accccgcaac cagagaatag
      121 cgggggtcgg gtgccccgtc atgatgtggc ggtcacgatt ccgacgatga ctttgaccac
      181 acgccggggg tcccagccga ccgaggcagt agggtgaaag tcatagtcca aaccgtgacc
      241 ggcgggacat gacaaccgta ctaacgtcca atatgctggg cataggcgct attgccacca
      301 ccctgtgagg agggatccat gacactagaa caggtcgtcc accgaatcgc aaccgattcg
      361 gcatttgcag cctccgttcg ggagtatccg gaggccgcgc taaaggggga ggccccggaa
      421 ctaagctcgt ccgagatccg gtcactcatg gctgcactca cggccgacga gacgcagaag
      481 gctccgggcg ctgcgctccc ctggtttgag tcgcagctcg ccagcaaccc cgacaacaga
      541 ccgcagaccg cgctgccctg gttcgaggcc caactgggaa tgcgacccgt atagtcaacc
      601 caactagcat cctcaacaca atcgaggcag cctggactgc cgctggcgca tggccggact
      661 tcgttcacgc catgcgccaa tctcttttgg ctggagaggg ccagccccag gcagcatccc
      721 cccgccgctc ctggctgtca ctccctggcc tctgctgccg ggcggcgggt ggtaatcctc
      781 aggatgcaga cggcgtagcg gcggcctggg tgctgctcta cactgccgcg catgtgttgg
      841 attcaatcca ggatcgagat ccgccagagc gctggtgggc cgagcttgga gaaggtccgg
      901 cgatcaacgt ggccacaggc ttgctatcaa ctgcctgggt cgttttagat cggatcgagg
      961 cggaccggag ttcggttcgg cgcgtgattg aggactttca cagcactgtc ttgtggatgg
     1021 cgagcggcca gcacctggat ctgacgctct cacagctcac cccagacaag gcttgggcga
     1081 tcgccgaagc caaatcggga gcgtttttcg cgctggcctg ccggtccgga gctcgcatgg
     1141 cgggagcggc cgaagcaact gcggagcagt atgggaggta cggactcaac ctgggtctga
     1201 tcgtccagat cggggacgac atggaagacc tggtccgcag ccagggcttt ccaccgttag
     1261 ctgtggcata ctgcctgcag acagcctccc cgaaggctcg aggacggctg agcactatca
     1321 tgcaggcgtc ttccgccggt ccatccgcgc aggtcgaggc tgccagaatc ctgacagcga
     1381 gcggagccct cctctacctt ggaacgaagc tccttcagtt tcaatccttc gctcttgaat
     1441 cgctcgatgc atccggcgcc gagccgacgg caagcaagga gctcacagcg ctagttcgag
     1501 cgttggcacc ctcggttgaa ccccgtccga cctaggaatg attagcctcg ccttccatag
     1561 gcttcgctac gcatttcctt ttctgcttgc aatcggatcc ctcgccctca tattttgggc
     1621 caccgacttc cagctccatc gcggctacga aggatttgac tggacgcccc tgtcgggtgc
     1681 cgttctcgac gtagatccgt tggcaccggc ctccgaaagc ctcagagtcg gagatcggat
     1741 cgttagtgtg gaaggcgtgc cggccatcct cgccccgcag atccatgcca atatcgggcc
     1801 gggcgacacg gtccattacc tgatcgaacg agaccggtct caattcgcgg tcgatctgag
     1861 gatggccgac cttcccccgc gggtggtttt tcaatccttg gtgcccttgg caattgctgc
     1921 gaccttctgg gcaatcgggt tggcggtgct gtcgttcggc caggctggag ccgaggcccg
     1981 gcctttcttt cggttctgct tgatagcaag cggcgtattg ggagccggtt cgctctcgac
     2041 cgccggaccg atatggagct caaccctctt caacatcctg ctctggttcg caggcccgat
     2101 cgcggtcgat ctgcatctgc ggttccccga ggcccgccgg cattcgcgtg caatgtcggc
     2161 ggtgctctat ggaggggcga tcctgggcag cctgcccttc ctactgctgg gagctaccgg
     2221 agttcgggcc agcgcctgga gccagttggt gttcacctcc ggccggctct tcctctcggg
     2281 gaacttgctg ctggtcgtgt ggctgctgct cagctcgtac cgccgggcaa ccagcgtaca
     2341 ggcccgccag cggatccgtt tggtggcgct cggcggcggc gcaggaatgc tgctggtcat
     2401 cgtgctcagc atcctgcccg gggcgctgac cgaccggccg ctcgtcccct acgaagtctc
     2461 cttcgccttc ttgatcgcac tgccggcctc ctacggctac gccatcgccc gccaccgcct
     2521 gatccggctg gaacggttcc tgagtcgcgg ggcggcttat acgctggttt tcgcgatttt
     2581 ggctgcgatc tatctcgggg taacgacagc cctcgaccga ctgttttccg atgagctgct
     2641 cgaacaaccg ctgatcaaca tgctggtgat cctactgctg gccgcgacgg cggtcgtgat
     2701 ctaccggcgg atccaggcgt tggtcgactt cgcgttttac ggcggctggt acgactaccg
     2761 ttcggcggtc gcgcggatca ctgaaggatt ggaagaacag aaaagcagcg aggagttggc
     2821 cgcgaccttg agcacccggt tgcaggccac gctgcggctc gagcgggcct gcgttttcgt
     2881 ggcaacccgg gacggttcat tggcggcgaa cggttgtgaa ctcccagacg aggccgccgc
     2941 gcccgccagc ccgctggccc tgcccgctca aggccaagtg gtccagtatc taaagctcaa
     3001 cccgcgtcca gtctccgccg aggcgttgcg ccagtccctc ctggaccgac cgctgactag
     3061 agacgagcgc ggcactctgc tggccacaac cgattgctta atggttccag tcgcagggag
     3121 cgacgggctg ttggcggtaa tggcactggg ccgccggcgt ggcggcgagg gattcagcgc
     3181 cgaagacctg gatatcctcg atctggtcgc ccgccacgcg ggggccgcga ttgagaatgt
     3241 gcgccttaca ggcgaggtgc gtcagcacgt tgtcgaggtc gagcagcttc acaagcgact
     3301 gctgcgagcc cgcgaagagg agcgcaagtc gctggcccgc gagctgcacg atgaggccat
     3361 acagtcgctg gtcggcatga actacaggct ggcccacctg gagagcggtc agactcagac
     3421 gttgaggcaa gaagtgctgc ggatcgtcga ggagctgcgc gggatgatcc accggctgcg
     3481 cccgccggcg ctggacaact tcggcctggt cacggcagtt cgctccgagt tgcgggaacg
     3541 gagcgtgcgg tcgcacggat ctcggcggat cgacctgcac gtcgaaggcg acccggaatt
     3601 ggggatctcg gaggaagtcg ggctgtgtct gtaccgggtg atgcaggagg cgctgaacaa
     3661 catcgagcgg cattccgagg ccgcccaggt cgatgtcgct ctggtgatgg gtccggaaga
     3721 gatcaggctc tcagtgcgtg acaatgggaa ggggtttcca gtccctcagc agctggggct
     3781 gctgctggag gccgaccact tcggcctggt aggcgtgcga gaacggttgg aactgctcca
     3841 tggtacgctg gagatcgatt cggctccggg cagtggaacg actctgcggg cgcgaatccc
     3901 tctagaatcg aactcgagcg gcccggcctg accgcgacca cgatggaaaa ctcagacggc
     3961 aagcacgccg acaagcccat ccgggtactg gtggcggacg accacccggt ggtgcgcagc
     4021 ggaattagag acgagctggc caagcacccg gatctactgc tccttgggga ggcagtggac
     4081 ggcgaccagg ccctcgagct ctcccgcacg ctgcggccgg atgtcctgct gctggatatc
     4141 aacatgcccg gtctgcagcc ggtgaaggtg gtgcgggagt tggccaagct taacggcggc
     4201 ccgcaggtcc tggtgctgag cgcctacagc gacgtcgagt acgtgttcga gatgctgaag
     4261 gccggggtgc acggctatct attgaaggac gaggaaccgg gacggatcgc agagggcatc
     4321 cgggcggtgg cggctggcaa gacctggctg agctc
//
LOCUS       DATDPQ010000314         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3039398, whole genome shotgun sequence.
ACCESSION   DATDPQ010000314 DATDPQ010000000
VERSION     DATDPQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039398"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="thrS"
                     /locus_tag="VI520_07060"
     CDS             <1..1422
                     /gene="thrS"
                     /locus_tag="VI520_07060"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HLF37676.1"
                     /translation="EISVYTHDSFTDLCRGPHVEHTGRINPDALKLINVAGAYWRGDE
                     RRTMLQRIYGTAWLTGEELEQHLWRLEEARRRDHRRLGRELDLFSISDQVGPGLILWH
                     PKGGLVRKIAEDFCRSEHERAGYQFVYSPHVGKAVLWETSGHLEWYKDGMFPPVELEG
                     QAYYLKPMNCPFHVEIYKSKTRSYRDLPQRYAEWGTVYRYERSGVLHGVLRVRGFTQD
                     DAHLFCRPDQMPEEIDRTLAFSLHILRSFGFQDFHAYLATRDPAKAAGSAEQWEAPTQ
                     ALQSALERAEIPYQIDEGEATFYGPKIDLKLMDALGREWQLTTIQFDFNLPERFELTY
                     VGEDGQEHRPYMIHRALLGSMERFLGTLIEHFAGAFPVWLAPVQATVIPIADRHAEYA
                     GRVANQLREAGLRVEVDDRGERMNAKIRDAQMQKVPYMLVVGDREAEAGKVAVRLRSE
                     EDLGAMSVEAFLDRAQHEIRQRV"
     gene            1574..>2337
                     /locus_tag="VI520_07065"
     CDS             1574..>2337
                     /locus_tag="VI520_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37677.1"
                     /translation="MSPTNRSSSTRSAARGQASAGAGPPEPPAFVPPYAPSWVDQFTD
                     WVERLPIPWWSFYVLLALGLSGAVALVLWRAGVYASFGFHPMQVWLPTLVAYLLGLTH
                     ALDRLAASALQRYRSAFRGGEAQFAAALHRITVLPARPTLILTVGLTIATIPIAIWEF
                     SYTQTGGLELVPALLPLLMGLVGLVSYPWLYRMLRQLREIHRLYRDHTEVRLTRVRPL
                     YAFSRVTALSAIGLVININGWFLAQPGQDPNNPVFL"
BASE COUNT          398 a          748 c          755 g          436 t
ORIGIN      
        1 gagatctcgg tttataccca cgacagcttc accgatctgt gccgcggccc gcatgtcgag
       61 cacaccggcc gcatcaatcc agacgccctc aagctgatca acgtggccgg ggcctactgg
      121 cgcggcgacg agcggcggac catgctgcag cggatctacg gcacggcctg gctgactggt
      181 gaggagctgg agcagcacct ctggcgactc gaagaggccc ggcggcggga ccatcgccgg
      241 ctcgggcggg agctcgatct ctttagcatc agcgaccagg tcggtcccgg tctgattctc
      301 tggcatccga agggcgggct ggtgcgcaag atcgccgaag atttctgtcg gtcggagcat
      361 gagcgagccg gctatcaatt cgtgtactcg ccgcacgtcg gcaaggccgt gctctgggaa
      421 accagcggcc atctggagtg gtacaaggac ggcatgttcc caccggtcga gctcgagggc
      481 caggcgtatt acctcaagcc gatgaactgt ccttttcatg tcgagatcta caagagcaag
      541 acccgctcct atcgggactt gccacagcgc tacgcggagt gggggacggt ctaccgctac
      601 gaacgcagcg gagtgttgca cggagtgctg cgagtccgcg gcttcaccca ggacgacgcc
      661 cacctattct gccggccgga tcaaatgccc gaggagatcg accgcactct ggccttcagc
      721 ctgcacatcc tgcgctcctt tggtttccag gatttccatg cctacctggc tactcgggat
      781 cctgccaagg ctgccgggtc ggccgagcag tgggaggctc cgacccaggc gctgcaatcc
      841 gcactcgaac gggccgagat cccttatcag atcgatgagg gggaggccac tttctacggc
      901 cccaagatcg atctcaagct gatggacgcg ctgggccggg aatggcagct gaccacgatc
      961 cagttcgact tcaacctgcc ggagcgattc gagctgacct acgtaggcga agacggtcag
     1021 gagcaccggc cgtacatgat ccaccgggcg ctgctgggat cgatggagcg cttcctgggc
     1081 accctgatcg agcattttgc gggagccttc ccggtctggt tggctccggt tcaggcgacg
     1141 gtgatcccga ttgccgatcg ccacgccgag tatgctggcc gggtcgcgaa ccagttgcgg
     1201 gaggcgggcc tgcgggtgga ggtcgacgac cgaggcgagc ggatgaacgc caagattcga
     1261 gatgcgcaga tgcagaaggt gccctacatg ctggtggtag gagaccggga ggcggaggcc
     1321 gggaaagttg cggtgcggct gcgcagcgag gaagacctgg gggcaatgtc agttgaggcc
     1381 tttctggatc gcgcccagca cgaaatccgg cagcgggtgt gagaataact gggcgtcgtg
     1441 acccgggccg gggtttcgcc gccgagcaat gttcgatggg atcggcgcac tgcggtgggt
     1501 cgtcacgaac cccctcggtt acaatcgctc gccggagttt gaaggagtca tccggcccgc
     1561 aggggactcg caaatgagcc cgacgaatcg atccagctcg acccgctctg ccgctcgcgg
     1621 ccaggcctca gccggcgctg gaccgcccga gccgccggcg ttcgtcccgc cgtacgcccc
     1681 gagctgggtg gatcagttca ccgattgggt cgagcgcctg cccatcccat ggtggtcttt
     1741 ctatgtcctg ctggcgctcg gattgagcgg agccgtggcc ctcgtgctgt ggcgggcggg
     1801 cgtctatgcc tccttcggat tccatcccat gcaggtgtgg ctgcccaccc tcgtcgccta
     1861 tctactcggc ctgacccacg ccctcgatcg cctggctgcc tcggccctgc agcgttaccg
     1921 ctctgcattc cgagggggcg aagcgcaatt cgcggcggct ctccatcgaa tcacggtcct
     1981 gcccgcccga cccacgctga tcctcactgt gggccttacg atcgccacta ttccgatcgc
     2041 gatatgggag ttctcctaca ctcaaaccgg aggcctggag ctggtgcctg cgctactgcc
     2101 tctcctcatg ggcctggtgg gcctggtcag ctatccgtgg ctctatcgca tgcttcgcca
     2161 gttgcgcgag atccatcggc tttaccggga ccacaccgag gtgcggttaa cgcgcgtccg
     2221 accgctgtac gccttttctc gagtgaccgc gctgagcgcc ataggcctgg tcatcaacat
     2281 caatggatgg ttcctggctc agcccgggca agaccccaac aacccggtgt tcctgat
//
LOCUS       DATDPQ010000315         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_691706, whole genome shotgun sequence.
ACCESSION   DATDPQ010000315 DATDPQ010000000
VERSION     DATDPQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691706"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VI520_07070"
     CDS             complement(<1..467)
                     /locus_tag="VI520_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HLF37678.1"
                     /translation="MALVSMLIAAGQFRVARAHTGNTYRVELTAAGFVPSQLEILAGD
                     TLLFENTDQSDHWPASDVHPTHQFYPDFDAQRPISPGDSWSFAFFRPGVWGFHDHLNP
                     QFKAQVVVLPDNHLISNDPSGTASGTSWLARYFSAARRFYERVFEAGKLFLAEV"
     gene            769..1896
                     /locus_tag="VI520_07075"
     CDS             769..1896
                     /locus_tag="VI520_07075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HLF37679.1"
                     /translation="MIPVSEPVLGDLEYEYVKECLETGWISSSGRFIHDFEGGWAEYC
                     RMKYGVAVSNGTVALQLALRAIELDPGDEVIIPSLTIISCALACIYNEAVPVLVDCDP
                     QTWCMDVEQVRQKITSRTRAVMPVHLYGHPVDMDPLMQLAEKHGLMVIEDAAEAHGAR
                     VLDRGGAGDWRPCGGLGHLSAFSFYANKPITTGEGGMVLTNDPELADRLRSLRNLSHR
                     SERRFYHTELGFNFRMTNLQAALGLAQLTRMEQIIERKRWIGKRYTETLAGVRGLQLP
                     VEREWAKQNYWMYGVVLDEATGQDAESLANRLAAQEIDTRPFFLGMHEQPALTDRGLF
                     TGEQYPVTERLSRQGLYLPSGLSLTESQLDFVCRSVTEALR"
     gene            1893..>3515
                     /locus_tag="VI520_07080"
     CDS             1893..>3515
                     /locus_tag="VI520_07080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF37680.1"
                     /translation="MKARVTAITLNPFSTPALLLVCLLLGTLSALRYFHQGVGGSVVL
                     IWLATIAGAGVVVFWADRNRSAPALIDRSDWPTALLLLLSVAPLYLALIPAIPYQIST
                     DEVVVMLVEGEASAQPVWDIFSLSEYFSFPSFVFVVYGWVGSLLGGINLQNMRSIDAI
                     VGLASIALFYILARPLLSKRMAAAAAVLLGTNHALLAISRMAMRNNSALLVELGGLLF
                     LFNGLKHRRASWTYLGGAITGLAFYIYEPARLLLIIWLGWLLAMAIGNRTRETTKLYL
                     RHGLIGLTGFALVVAPLVAGSMRATENPAAFAYQREQLLFFRESQLQQQEWVGAATLV
                     EAVQTNIVQGLTAYNSGLHDNGYLYNNPGHGFVDPLTGILLWVGVLWTVSRLGERKSE
                     DYLMLVGFVVPWLTFSFITNKAPSYTRMLITLPFVAYMTLRGIVFVAGRFHLLVSKRW
                     PAAGKLLSGRLLAGGLVAAIALWNLGSYADYVVTGLVERENVGATSRYIQTRLTDEDH
                     AFYLAADHQHEFFGGGEPWHWEAWVRFFAGDDV"
BASE COUNT          639 a         1027 c         1127 g          722 t
ORIGIN      
        1 acctcagcca ggaagagttt gcccgcctca aagacccgtt catagaatcg gcgtgccgcg
       61 ctgaagtagc gagccaacca gctcgttccc gaagcggtcc cgctcgggtc gttggaaatg
      121 agatggttat ccgggagcac aacgacctgg gccttgaatt gtggattcag gtggtcgtgg
      181 aacccccaga cccccggccg aaagaaggcg aagctccaac tgtcgccggg gctgatcggc
      241 cgttgggcgt caaagtcggg atagaactga tgagtggggt gaacatcgga ggccggccaa
      301 tgatcggatt gatctgtgtt ctcaaacagc agagtgtcgc cggccaggat ctcgagctgg
      361 ctaggcacga agccggccgc ggtcagctct acccggtatg tattcccggt gtgggcgcgg
      421 gcgacccgaa actggccggc cgcgatcagc atcgagacca gcgccacgca tccaaggcgg
      481 aggaggcgtc gggcgtggct tgaatgaatg ggtcctggcg gcttagctag catgcgtgtg
      541 ggcccgatcc aggcccgggt cgaccacggg atggtcgacc ggtcgggtct ggcaatggga
      601 agaccctcga cgatattagc actcgcccct ggctggggcc agttggctgg tacataatta
      661 ccagccgggc agacgaaaac ctgtccgcat gatcgtccac agccccgagt agggcgggaa
      721 ttggacaaag ttgacactgg agttcaacct agtaggatac gttctgcgat gattccggtc
      781 agcgagcccg tgctgggcga cctcgaatac gagtacgtca aggagtgcct ggagaccggc
      841 tggatctcct cttccggacg attcatccat gacttcgagg gcggctgggc cgagtactgc
      901 cgaatgaaat acggggtggc ggtcagcaac gggacggtcg ccctgcagct ggccctgcgg
      961 gcaatcgagc tggatcccgg cgatgaagtc attattccgt ccctgacgat catctcctgt
     1021 gccctggcct gtatctataa tgaagcggtt ccggtgctgg tggactgcga cccccagacc
     1081 tggtgcatgg atgtcgaaca ggtgcggcaa aagatcacct ctcgcacccg ggccgtgatg
     1141 ccggttcacc tctacggtca tccggtcgac atggatccgt tgatgcagct ggccgaaaag
     1201 cacggtctga tggtgatcga agacgctgcc gaggcgcacg gggcgcgggt gctcgaccgc
     1261 ggtggagctg gtgactggcg gccatgtggc ggactgggtc atctcagtgc gttcagcttc
     1321 tacgccaaca aacccatcac caccggcgaa ggcgggatgg tactcaccaa cgaccccgag
     1381 ctggccgatc ggctacgctc gcttcgcaat ctctcccacc gcagcgagcg gcgcttctac
     1441 cacaccgaac tcggattcaa tttccggatg acgaacttgc aggcggccct cggcctggcc
     1501 cagctgactc ggatggagca gatcatcgag cgcaaacgct ggatcggaaa acgctataca
     1561 gagactctgg ccggggtccg ggggctgcag cttccggtgg aacgggaatg ggcgaagcag
     1621 aattactgga tgtacggggt cgtgctcgat gaggcgactg gccaggacgc cgagtcgctc
     1681 gccaatcggc tggccgcgca ggagatcgat acccggcctt tctttctggg gatgcatgag
     1741 cagccggcct tgaccgaccg agggctattc accggggagc aatatccggt gactgagcgc
     1801 ctgtctcggc aggggctgta cttgccctcc gggctctcct tgactgagag ccaacttgac
     1861 ttcgtgtgcc ggtcggtgac cgaggccctc cgatgaaggc cagggtcacc gcgattacgc
     1921 tcaatccatt ctccaccccc gccctgctgt tggtgtgcct cctgctaggc accctctcgg
     1981 ccctgcgtta ctttcaccaa ggggttggtg gttcggtggt gctgatctgg ctggcgacga
     2041 tcgcaggcgc gggggttgtc gtcttttggg cggatcgcaa tcgctcagct ccggctctga
     2101 tcgaccggtc ggattggcct accgcgctgc tcctgctctt gtccgttgcc ccgttgtatc
     2161 tggcgctcat tccggctatc ccctatcaga tcagcacgga tgaggtcgtc gtgatgctgg
     2221 tggagggcga ggcctccgcc caacccgtct gggacatctt ctcgctgagc gaatacttca
     2281 gctttccctc cttcgtcttc gtggtctacg gttgggtcgg cagtctgctt ggcgggatca
     2341 atctgcagaa catgcgcagc atcgatgcga ttgttgggct ggccagcatc gcactcttct
     2401 atatcctggc gcgtccactg ctgagcaaac ggatggcggc cgcggcggcc gtcctgcttg
     2461 gtacgaacca tgcgctgctg gcgatcagcc ggatggcgat gaggaacaac tcggccctgc
     2521 tggtcgagct cggtggcttg ctttttctgt tcaacggtct gaagcatcgg cgagcgagtt
     2581 ggacctatct gggcggggca atcaccggat tggcattcta catctacgaa ccggcccgcc
     2641 tcctgctgat catctggttg ggctggctgc tggcgatggc gattgggaac cggacccggg
     2701 agacgaccaa gctctacctg cggcacggcc tgatcggcct gaccggattt gctctggttg
     2761 tcgctccatt ggtggccggc tccatgcggg caacggaaaa cccggcagca ttcgcctacc
     2821 agcgcgaaca gctgctgttc ttcagggagt cccaacttca gcagcaggag tgggtcgggg
     2881 cggccaccct ggttgaagcc gtccagacga acatcgtcca gggtctgact gcttacaaca
     2941 gcggattaca cgacaacgga tacctctaca acaacccagg ccacggattc gtcgacccgc
     3001 tgaccgggat cctgctctgg gtaggagtgt tgtggacggt ttccagattg ggtgagagga
     3061 agtcggaaga ttacctgatg ctggtcgggt tcgtcgtgcc gtggctcacc ttcagtttca
     3121 tcaccaacaa ggcccccagc tacacccgga tgttgattac gcttccattt gtggcctata
     3181 tgaccctgcg tggaattgta ttcgtggcag gtcgcttcca tctgttggtc tccaagcgat
     3241 ggccggctgc cggcaaactc ctctccggca gattgctcgc tggcgggctg gttgcggcaa
     3301 tcgcgttgtg gaacctcggc agctatgctg attatgtggt caccggcctg gtcgaaaggg
     3361 agaatgtggg cgcgaccagc cgctacatcc agaccagact cactgacgag gatcatgcct
     3421 tctatctggc ggccgaccac caacatgaat tcttcggggg aggagaaccc tggcattggg
     3481 aggcgtgggt caggttcttt gccggggatg atgtg
//
LOCUS       DATDPQ010000316         2935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_423178, whole genome shotgun sequence.
ACCESSION   DATDPQ010000316 DATDPQ010000000
VERSION     DATDPQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423178"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..939)
                     /gene="trxB"
                     /locus_tag="VI520_07085"
     CDS             complement(16..939)
                     /gene="trxB"
                     /locus_tag="VI520_07085"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLF37681.1"
                     /translation="MKQEQIVIIGSGPAGLTAALYTARAQLEPLVISGSQLGGQISIT
                     SEVENYPGFPNGTTGPELVELMRAQAELFGARLLIDEVTEVDFRNGPPFTVKSHSETY
                     EAKAVIVTVGASPIRLGVPGEEEFIGRGVSFCATCDGFFFRDKEVVVVGGGDSAIEEG
                     IFLTRFANQVQVVHRRDRLRAGEALKRRAMDNEKIAFVWDSVVEEIVGNGKVEAIRLR
                     NLKTGDRQQMPTDGVFIFIGHYPNSSLFEDQLKMDEKGYLTVDERFMTSVPGVFAAGE
                     IMDPVYRQIATSVGQGCSAAMMAERWLREHS"
     gene            complement(1044..1625)
                     /locus_tag="VI520_07090"
     CDS             complement(1044..1625)
                     /locus_tag="VI520_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37682.1"
                     /translation="MNLRWESVVWVALAVASPALATKFWPRLRERFGDRAELVERFAP
                     WAHGFGPAYLALLSGAVRAREAGLLGHAPLAWVVGALASAVWLWLVTFRRRNVSRWPE
                     PNRGVLDEPRWTLYRAVGSQWVGDPQIGGLVGLALAISEWGLGGRAQGRVEWQTLARL
                     ASSSVVFALTRNFWLTALAQAGLLILLRRGRNT"
     gene            complement(1699..>2935)
                     /locus_tag="VI520_07095"
     CDS             complement(1699..>2935)
                     /locus_tag="VI520_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLF37683.1"
                     /translation="TPAAAGLGHWLRLRTLATRPDPGPEGGAHQGLTPTMLGLATELN
                     SELRRERIPQLVVNLALGGFDQGIRMAVLLRGEDGYGLAAGHPPVGDRSIVLPGQEGL
                     LAEALATGSPTISQQAVDDPAFERLGLAVGARAWVCLPLMNHGQPEGALLLAHPMAGY
                     FHPGRLDLVKAIGAQASSALNNARLVRELELERDRISETEEETRRKLARNLHDGPTQT
                     IAAIAMRLNFARRLVRRDAVAAESEIQTLEEIARQTTKEIRHMLFTLRPLILESKGLV
                     AALHQLASKLEETHGQRVEVEAQPGVADGLDLSKQAVIFFIAEEAVNNAHKHGEAANI
                     RVGLHKVGGDRIALEVWDDGVGFNVGAVDANYEQRGSLGMVNMRERSELINGVLRIES
                     AEGRGTHITLEVPLASNNG"
BASE COUNT          541 a         1019 c          837 g          538 t
ORIGIN      
        1 acctggcaac cgcgttcacg aatgctcgcg cagccagcgt tcggccatca tcgccgccga
       61 gcagccctgt cccaccgagg tggcgatctg acgatagacc ggatccatga tctcgccggc
      121 cgcgaacacc cccggcacac tcgtcatgaa ccgctcgtcg acggtcagat atcccttctc
      181 atccatcttc agttggtcct cgaataggct ggagttggga tagtggccga tgaagatgaa
      241 cacgccgtcg gtgggcatct gctgccggtc tccagttttc agattgcgga gtcggatcgc
      301 ctccactttt ccattgccaa cgatctcctc caccacggag tcccagacaa acgcgatttt
      361 ctcattgtcc atggcgcgcc gtttcagcgc ctcgccagcc cgcagccgat cccgtcgatg
      421 gaccacctga acttgattgg caaagcgggt gagaaagatc ccttcttcta tggcactgtc
      481 gcccccaccc accactacca cttccttgtc acggaagaag aacccatcac aggtcgcaca
      541 gaacgacact ccccggccaa tgaattcctc ttcccctgga acgccaagcc ggatggggga
      601 ggcacccaca gtcacgatga ctgccttggc ctcataggtc tcgctgtggc ttttcaccgt
      661 aaacggcggc ccattcctga aatcaacttc ggtcacctca tcgatcaaga gcctggctcc
      721 gaagagctcc gcctgggctc gcatcaactc tacgagctcg ggcccggtcg taccgttcgg
      781 gaagcctggg tagttctcga cctcactggt gatcgagatc tggcctccca actggctgcc
      841 agagataacc agcggctcga gttgagcacg ggcggtgtac aaggccgcgg ttaaaccggc
      901 cggacccgat ccaatgatga cgatttgctc ttgcttcatg cacttccctc atgcactgcc
      961 gaggatttcc cctatgtgga gcacggccgc gcctgaattc accgcctgac cattgtacgc
     1021 gaccggccga cccgccacaa cgactaagtg ttgcggcctc gccgaagcag gatcagcagc
     1081 ccagcctgtg ccagggcggt cagccagaag ttccgagtga gggcaaacac gaccgatgag
     1141 ctggcaagcc gggccagagt ctgccactcg acccgcccct gcgcccgacc gcccaacccc
     1201 cattctgaaa ttgccaatgc cagaccgacc agtccgccga tctgaggatc ccccacccat
     1261 tggctgccga ctgcccgata tagcgtccag cgcggctcat ccagcacccc gcggttcggc
     1321 tccggccagc gcgatacgtt tcgcctgcgg aaggtaacca gccagagcca gaccgcgctg
     1381 gcgagcgcgc cgacgaccca agccagggga gcatgcccga gcagcccggc ctcccgagcc
     1441 cgaacggcgc cgctcagcaa tgcgaggtag gcaggaccga atccgtgggc ccagggagca
     1501 aagcgctcta ccagctccgc ccgatccccg aaccgctctc taagccgcgg ccagaacttc
     1561 gtcgccagcg cgggcgaggc tactgccaag gccacccaca ccacgctctc ccagcggagg
     1621 ttcaaataat tggctcgggg ttagattgta ctgagccgcg cccggcttac cggccggtga
     1681 gggagtcgcc ggtgcccgtt agccgttgtt gctggctaac gggacctcca gagtgatgtg
     1741 ggttcctctg ccctcggcgg actcgatccg cagcactccg ttgatgagct cagagcgctc
     1801 gcgcatattg accatgccca gactgccgcg ctgttcgtag ttggcgtcca ccgcgcccac
     1861 gttgaacccc actccgtcat cccagacctc cagcgcgatc cggtctcctc cgactttgtg
     1921 caacccaacc cggatgttgg ccgcctcgcc atgtttatga gcgttgttga cggcctcttc
     1981 ggcaatgaag aagatgaccg cctgtttgga gaggtccagc ccatccgcca ccccgggttg
     2041 agcctcaacc tcgactcgct gaccgtgagt ttcttccagc ttgctggcca gttgatggag
     2101 ggctgccacc aggcccttcg actcaaggat caacggccgt agggtgaaca gcatgtgccg
     2161 gatctctttg gtcgtctggc gggcgatctc ctccagggtc tggatctcgg actcggccgc
     2221 caccgcatcc cgccgaacca gccgccgggc gaagttcagt cgcattgcga tcgcagcaat
     2281 ggtctgagtt ggcccgtcgt gaaggttgcg agccagcttg cggcgggttt cttcctcggt
     2341 ctcgctgatg cgatctcgtt ctaactcgag ctcccggacc aggcgggcgt tgttgagcgc
     2401 gctgctggcc tgagcgccga tggccttcac aaggtccagc cggccgggat gaaagtagcc
     2461 cgccatcgga tgggccagca agagtgcacc ttcaggttga ccatgattca tcagcggtag
     2521 gcacacccaa gctcgcgccc ccaccgccaa tcccaaccgc tcgaaagctg gatcgtcaac
     2581 ggcttgctgt gaaatcgtgg ggctgccagt cgccagtgcc tcagccagca acccttcctg
     2641 gcccggcagc acaatcgagc gatccccaac tgggggatgc ccggcggcga gaccatagcc
     2701 gtcctcgccg cgcagcagga ctgccatccg aatgccctgg tcgaaccctc ccagcgcaag
     2761 gttgacaacc agctggggga tccgttcgcg ccgcagctcg gaattcagct cggttgccag
     2821 tcccaacatc gttggggtca gaccttggtg cgctccaccc tctggccccg gatcgggcct
     2881 ggttgcgagc gttctcaggc ggagccagtg ccccagaccc gcggcggccg gggtg
//
LOCUS       DATDPQ010000317         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1749209, whole genome shotgun sequence.
ACCESSION   DATDPQ010000317 DATDPQ010000000
VERSION     DATDPQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749209"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VI520_07100"
     CDS             <1..268
                     /locus_tag="VI520_07100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLF37684.1"
                     /translation="LDQLRAVVPLDDARVLEIGCGDGRLTWKYASQSRAVLAIDADRS
                     KMAAARAELSVRRLSAEVQLAQAAAESLPVRREAFDLAIFSWSL"
     gene            287..709
                     /locus_tag="VI520_07105"
     CDS             287..709
                     /locus_tag="VI520_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37685.1"
                     /translation="MVHALEQAWQALRPGGVFVDLRPLSLDSALDIVGSQGMESAGMV
                     DMSADLPDDRAVDEAIEAAVRAEVFIPLESRRFDFAYYWESFEGMRRYTAERWDTAKF
                     PPAVLRKARALKNSLGPDSRIRIILPMHLATYERARLR"
     gene            706..1314
                     /locus_tag="VI520_07110"
     CDS             706..1314
                     /locus_tag="VI520_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF37686.1"
                     /translation="MSGPASIEIRELRAEAEELSLQVAGLLLEAFREHWPAAWPTLEA
                     ARQEVRDSFGPDRISRIAVDSDGSVLGWIAGHEQYDGHAWELHPLVVHPAHQLQGIGT
                     VLVQDFETQVRARGATTIWLGTDDEDNLTSLGGADLYPDVLERASKIRNLRSHPFGFY
                     QKLGYAIVGILPDANGPGKPDILMAKRVGSFQPGRNDRQQDG"
     gene            1307..1750
                     /locus_tag="VI520_07115"
     CDS             1307..1750
                     /locus_tag="VI520_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37687.1"
                     /translation="MAERPVAPAGYSARTLPEKLGFKSGDRAAILYSPQGYLARLGEL
                     PTQVKLLSGRARQLDFIQMFARSRTDLERTFAKAAARLKPDGMLWISWPKQSSPLAGD
                     LNENIVRELGLALGLVDVKVAAIDYDWSGLKFVYRLKNRPTRSGS"
     gene            1807..2457
                     /locus_tag="VI520_07120"
     CDS             1807..2457
                     /locus_tag="VI520_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HLF37688.1"
                     /translation="MKKLDLRKELKHLYSPSAKQVEIVVVPSFKFLMLDGRIEPGRAP
                     EDSPSFRAAFGALYGMSFTLKFMSKLNKAKPIDYKLMALEGLWWTKSREFRVDRKDDL
                     RWTMMILQPNHITKAMVTEAMQQMKAKEKAKNREPNPALDLLRFEDFKEGTCMQIMHV
                     GPYSEEPRTVERLMAFAGEHGYVRRGKHHEIYLGDPRKSKPERLRTILRQPVKKAS"
     gene            2508..3317
                     /locus_tag="VI520_07125"
     CDS             2508..3317
                     /locus_tag="VI520_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF37689.1"
                     /translation="MAVASARLRFDHTLVAVFDLAAAKSEFEALGFTVISGGQHSGGL
                     TENALVTFADGNYLELIAPTSLELLDRPPQPGPGNYLFLFSSGEGFAGYAFDASNLRA
                     EVARVRSNGIDLGDPQPGGRRRPDGVKLAWRTAMLPDTRSPFFLTDDTPRDRRVREDF
                     EITTHPNGAVGVIGIVIPVENLERAVAWHEVLLGVPSRPGSSGVGAATAEFQIGGFGL
                     TLAEPEGKPSPLADHLARRGQVPYQVRIRTTNLLHVAGLEARGARIELVQG"
     gene            3368..3829
                     /locus_tag="VI520_07130"
     CDS             3368..3829
                     /locus_tag="VI520_07130"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HLF37690.1"
                     /translation="MSPYLFLKWIHVLAAITALGANLTYVVWIRLGERSQPNLLHSLR
                     GIQFLDARIANPAYGVVVVTGLLMVWVNKLSLLTSWITIPLILVVVVTVLGIAVYSPA
                     IRQQIQAAEADGPTSASYQTASRRAFLAGALATLLVVAIVFVMVVKPVLWS"
     gene            3860..>4122
                     /locus_tag="VI520_07135"
     CDS             3860..>4122
                     /locus_tag="VI520_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37691.1"
                     /translation="MAEVRARRKYWLRLGLTAVVALVTVSTVAIVMVARSRAAELISH
                     PRDQREPLEGSPADMGLPYEDVEVVSVDGFRLVGWYVPTQNGAV"
BASE COUNT          771 a         1181 c         1356 g          814 t
ORIGIN      
        1 actcgatcag ctgcgggcag tcgtgccgct cgacgacgcc cgagtgctcg agatcggatg
       61 tggggacggc cggctcacct ggaagtacgc gagccaatcc cgggcggtgc tggcgatcga
      121 cgcggatcgc tcaaaaatgg cagctgcgcg ggccgagctg tccgtcagac ggttgtccgc
      181 cgaagttcag ctcgcccagg ccgcggccga gtcgctgccg gtccgccgag aagccttcga
      241 tctagctatc ttctcctggt cactttgatg catcgctcac ccgggcatgg ttcatgccct
      301 cgagcaggcc tggcaagcgc tgcgccccgg cggagtgttc gttgatctac ggcctctctc
      361 actggactcg gccttggata ttgtgggttc tcaagggatg gagtccgccg ggatggtgga
      421 catgagcgct gaccttccag acgaccgggc tgtggatgag gcgatcgagg cagctgtgcg
      481 ggccgaagtg ttcattccgc tggaatccag gaggttcgac ttcgcctact actgggagag
      541 cttcgagggg atgcgccgat acaccgcaga acgatgggat actgccaaat tcccgcctgc
      601 agtgttgcgg aaagccagag cgttgaagaa tagcctgggt ccggattccc ggatccgaat
      661 tatcctccca atgcacctgg ctacttatga aagagcgcgg ctaaggtgag cggtccggca
      721 tcgatcgaga tccgggaact gcgggccgag gcggaggagc tttccctgca ggtggccggc
      781 ttgctgctcg aggctttccg cgagcattgg cccgccgcct ggccgacgct agaggcagcc
      841 cggcaagagg ttcgagactc gtttggacct gaccgcatca gccggatcgc cgtcgactcg
      901 gatggctcgg tcctcggctg gatcgcaggg catgaacagt atgatggcca cgcctgggaa
      961 ctgcatcccc tggtggtcca tccggcccac cagctccaag ggatcggaac ggtgttagtc
     1021 caggatttcg agactcaagt gcgggcccgc ggcgcaacga cgatctggct gggcaccgac
     1081 gatgaggata acctgacctc cctcggtgga gcagacctgt accccgatgt ccttgagcgg
     1141 gcctccaaga tccgaaactt gaggagccat cctttcgggt tctaccagaa gctcggctat
     1201 gcgattgtgg ggatcctccc cgatgcgaat gggcccggca agccggacat tctcatggcg
     1261 aagcgggtcg gatcgttcca gccagggcgc aacgatcggc agcaggatgg ctgaacggcc
     1321 tgtcgcgccg gccggatact cggcccggac actcccggag aagctgggct tcaagtcggg
     1381 agaccgggcc gcgatcctgt attcccccca gggctatctt gcaaggctcg gcgagttgcc
     1441 aacccaagtc aagcttctga gcggccgggc caggcagctc gacttcatcc agatgttcgc
     1501 ccggtcacgg acggatttgg agcgaacctt cgcgaaggcg gcagcccggt tgaagcccga
     1561 tggcatgctg tggatctcct ggccgaagca atcctccccg ttggctggcg accttaacga
     1621 aaacatcgtg agggaactcg gcttggccct cggactggtt gatgtgaaag tagccgccat
     1681 cgactacgat tggtcgggtc tgaagttcgt ataccggctg aagaatcggc cgacgcgctc
     1741 aggatcatga actgagtggc gtaccccgga tgccagtgag ctgaggaggc cagtcgattg
     1801 ccgacggtga agaagctcga tctccgaaaa gagctgaaac acctgtactc gccgtcggcg
     1861 aagcaggtgg agatcgttgt agtcccgtcg ttcaagttct tgatgttgga tggaagaatt
     1921 gagccgggac gggccccgga agattcgccc tcgttccggg ccgcgttcgg ggcgctgtat
     1981 gggatgtcct tcaccttgaa gttcatgtcc aagttgaaca aggccaagcc cattgactac
     2041 aagctcatgg cgctcgaagg gctgtggtgg actaagtctc gggagtttcg ggttgaccgg
     2101 aaggatgacc tgcgctggac catgatgatt ctccaaccga accacatcac caaagccatg
     2161 gtgaccgagg ctatgcagca aatgaaggct aaggagaagg ccaagaaccg cgaaccaaac
     2221 ccggcactcg atttgctcag gtttgaggat ttcaaggagg ggacctgcat gcagatcatg
     2281 cacgtcggcc cgtactccga agagccccgg accgtcgaga gattgatggc attcgctggc
     2341 gagcacggct atgttcgtcg cggcaagcat cacgagatct acctgggcga cccacgcaag
     2401 agcaagccgg agcgattgag gacgatcctg cgacaaccgg tcaagaaggc tagctgagtg
     2461 catctgccga cgttatatgg aagcaagatc agaatcgtgg gtggaatgtg gccgtggcct
     2521 cagcccggct gaggtttgac cacaccctcg tcgcggtctt cgatctggcg gccgcgaagt
     2581 cagaattcga ggcgcttggt tttacggtca tctctggcgg ccagcactcc ggcgggctca
     2641 cggagaatgc gttggtgacc ttcgcggacg gcaactatct ggaactcatc gctcccacga
     2701 gccttgagct gctggaccgg ccgccgcaac cgggccccgg caactacctt tttctgttca
     2761 gttctgggga gggtttcgcc ggatatgcgt tcgacgcttc gaatctgaga gccgaagtgg
     2821 cacgggtccg ttcgaacggc atcgacctcg gcgatccgca accgggcggt cggcggcggc
     2881 ccgatggagt gaagctggcc tggcggactg cgatgctgcc cgacacccgt tcgcccttct
     2941 tcttgaccga tgacacgccg cgggaccggc gagtccgaga ggacttcgag atcaccacgc
     3001 acccgaacgg cgcggtgggt gtaatcggga tcgtgatccc ggtcgaaaac ctggagcggg
     3061 ccgtggcttg gcacgaagtg ctcctggggg tgccctcgag gccgggttcg tctggagttg
     3121 gagccgccac tgccgaattc cagatcggag gctttggcct gactttggct gagccggagg
     3181 gcaagccgag cccgttggcg gatcatttgg ccaggcgggg ccaggtgccc taccaggtcc
     3241 gaattcggac aaccaacctt ctgcatgttg ctggtctgga agcccgaggg gcgcgaatcg
     3301 agctggttca aggttgagtg gattgtagcg tttttcacaa taagaatctg aggtagcaag
     3361 agagaccatg agcccatacc tgttcttgaa atggatccac gtgttggcgg ccatcactgc
     3421 cctcggtgcg aacctgacct acgtggtatg gattcgcttg ggggagcgca gccagccgaa
     3481 cttgctgcat agcctgcgag gcatccaatt cttggatgcc cggatcgcca acccggccta
     3541 cggagtagtg gtggtcaccg ggctgctcat ggtctgggtc aacaaactgt ctctgctcac
     3601 ctcttggatt acgatacccc tgatcttagt ggtcgtggtg accgtgttgg ggattgcggt
     3661 ctactccccc gcgataaggc agcagatcca ggcggcggag gccgacggcc ccacctctgc
     3721 ctcttaccaa actgcctccc gccgggcttt cctggccgga gccctcgcca ccctgctggt
     3781 ggtggcaatt gtgttcgtga tggtggtcaa gcccgtcttg tggagttagg tcgggtgatc
     3841 catagggtgg ggaggcgtca tggctgaggt tcgggctcgc cggaaatact ggttgaggct
     3901 tgggctcacc gcggtcgtgg cgttggtcac tgtcagcacg gtggcgatcg tcatggtcgc
     3961 ccgctcgagg gcggccgaac tcatctcgca tccgcgggat cagcgcgagc cgttggaggg
     4021 ctcgccggcc gacatggggt tgccttacga agacgtggaa gttgtatcgg tagatggatt
     4081 ccggttggtc ggttggtatg tgccaactca gaacggcgcg gt
//
LOCUS       DATDPQ010000318         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_693607, whole genome shotgun sequence.
ACCESSION   DATDPQ010000318 DATDPQ010000000
VERSION     DATDPQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693607"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VI520_07140"
     CDS             <1..817
                     /locus_tag="VI520_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLF37692.1"
                     /translation="GIPRSTPPKPGGAVGGVLRLSELGLDAFEIGWVQSVRVKVETCQ
                     KIKEAAAAADVAISVHAPYFINLNATKEEWPKSRKRLMDAAHYGNLAGATDISFHPGS
                     YFGLPPDQVLEVAIPRLQGCIDELRAADNPAMLRPETMGKSALLGTLADALVLAKEIE
                     GVLPCVDFAHLHARAGDGTVNSYPEWVVALTSYKKALGAQAMKRMHIHLSGIQYGPKG
                     ERNHLMLAESDFDLNGLLRALREFNCQGRILCESPVMEQDAAVIKKGWQRVAK"
     gene            complement(906..1586)
                     /locus_tag="VI520_07145"
     CDS             complement(906..1586)
                     /locus_tag="VI520_07145"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HLF37693.1"
                     /translation="MNRVRVATIGIVLLSQLVPSAAFAEEEHPQRERFRYAGEITGVV
                     PGQGTFTLLTRAGEELEFQTSDRTHYRSPDGSVQEIHDLKKGMKALVAGIKDEDVLNA
                     LLVAAGNPEDRPERPRVDVRAAGRVDSKGGGSFSLMTREGELLTFAVTQDTHYKGIGS
                     FEELEVGMFAAVGAKQTDGDLVAIWVAARQAPEDRPRPEDRPGRNPEDRPDRRPDQAP
                     NPDEQVLA"
     gene            complement(1697..2341)
                     /locus_tag="VI520_07150"
     CDS             complement(1697..2341)
                     /locus_tag="VI520_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HLF37694.1"
                     /translation="MAKHFVLVAGNIGTGKTSLAAKLGERLGWETAFESVEDNPYLAD
                     FYADMPSWAFHLQIFFLGHRAHQHLQFARATRSAIIDRSIYEDAEIFAKAALELGSVT
                     ERDYRTYRQVFDLVVGTLPKPDLLIFLKAELPVLLERIGARGRDMERGLSAEYLGLLE
                     GLYADWMTNFDLCPTLTIRTDDLDFVHEPRHLALIVGRIRDKLAGKEEIAFPTE"
BASE COUNT          482 a          780 c          783 g          488 t
ORIGIN      
        1 tggcattccg cggtccaccc cgcctaagcc gggcggcgcg gttggcggcg tgcttcgatt
       61 gagcgagctg gggctggacg cctttgagat tggatgggtg cagtcggtac gggtaaaagt
      121 ggagacctgc cagaagatta aagaggctgc ggcggcggcg gacgtggcga tcagcgtcca
      181 cgcgccctac ttcatcaatc tgaacgcaac caaggaggag tggcccaaga gccgcaagcg
      241 actgatggac gccgcccact acggcaactt ggccggcgcg accgacatca gcttccaccc
      301 gggctcgtat ttcggtctgc cgccggatca ggtgctcgaa gtcgcaatcc ctaggctgca
      361 gggttgcatc gatgagctgc gggccgcaga caatcctgcg atgctgcggc cggagacgat
      421 gggcaagagc gccctgttgg gcaccctagc cgatgcgctg gtgttggcga aggagatcga
      481 aggcgtcctt ccgtgtgtcg acttcgccca cctgcatgcc agggccggcg atggaaccgt
      541 gaactcatac cccgagtggg tcgtcgcact cacgagttac aagaaagcgc tcggggccca
      601 ggcgatgaag cgaatgcaca tccatctctc gggcatccag tacggcccca agggcgagcg
      661 aaaccacttg atgctggcgg agtccgactt cgatctgaat ggtctgctga gggcgctgcg
      721 ggagttcaac tgccaggggc ggatcctctg cgagagtccg gtcatggagc aggatgcggc
      781 cgtcattaag aagggctggc agcgggtagc caagtagctc cccgctgagc aactgctcat
      841 gaactcaaaa tggggctgcc gattgcggca gccccatttt gcttcttagc tctgatggga
      901 tccggttagg ccaagacctg ctcatccgga tttggagcct gatccggtct gcggtctgga
      961 cgatcctccg ggtttcggcc cggccgatcc tccggccggg gccggtcctc gggagcctgg
     1021 cgggccgcaa cccagatcgc aaccaggtcg ccgtcggttt gcttggcgcc taccgccgca
     1081 aacataccga cttccaactc ctcaaaactg ccgattccct tgtagtgagt gtcttgggtc
     1141 acggcgaagg tgagcagctc gccttcacgg gtcatgaggc tgaacgatcc accgcccttg
     1201 gaatcgactc ggccggccgc gcgcacatcg actctcggcc gctccggacg atcctccgga
     1261 ttcccagccg ccacgagcag cgcattcaga acgtcttcgt ccttgatccc tgcgaccagg
     1321 gccttcatcc cctttttcag gtcatgaatc tcctggaccg aaccgtccgg actgcgatag
     1381 tgggttcgat cgctggtctg gaactcaagc tcttcaccgg ctcgggtcag cagggtgaaa
     1441 gtgccctggc ctggcacgac cccggtgatc tcgccagcgt accgaaatcg ctcgcgttga
     1501 gggtgctctt cctcggcgaa cgcggcactc ggaaccaact gcgagagtag tacgataccg
     1561 attgtggcta cacgcactct gttcatcatg atccacctcc gtggtgaaga tcaggatggc
     1621 ggatgcgcgc tatggccagt tgatgggact gtaaaaagtg ggtaaattgt gggtcctgat
     1681 ggaggccgct cagcccctac tcggtcggga aggcgatctc ttctttgccg gccagtttgt
     1741 cgcggattcg gccgacgatg agggccaggt gacggggctc gtgaacaaag tccagatcat
     1801 ccgtccggat ggtcagcgtg gggcacaggt cgaagttcgt catccaatcg gcatacagcc
     1861 cctcaagcag cccaagatac tcggccgaga gcccacgctc catgtcccgc ccccgcgccc
     1921 cgatccgttc cagcaagacc ggcagctctg cctttaagaa gatcaacagg tcgggtttcg
     1981 ggagcgtccc gacgaccaga tcgaagactt gtcggtaggt tcggtagtcg cgctcggtta
     2041 cgcttcccag ctccaacgcg gccttggcaa aaatctccgc atcctcatag atcgagcgat
     2101 cgatgatcgc cgatcttgtc gcccttgcga actggagatg ctggtgcgct cgatggccca
     2161 ggaagaagat ctgcagatgg aatgcccagc ttggcatgtc ggcgtagaag tccgccaggt
     2221 atggattgtc ttcgaccgat tcaaaggccg tttcccagcc taggcgctca ccgagcttgg
     2281 cggccaggga ggttttgccg gttccaatat tgcccgccac cagcacaaaa tgcttcgcca
     2341 tccgatcgcc tcttggcctg ccgcccctag gatagcacgc agttcgccac ctcctggcca
     2401 gcgccgcaga actcgggcgg agcgcatcga aagccagact tgggtagact ctcggaatat
     2461 ggacccgccc gaccggccga tgagcggcaa agttgcactg gtaaccggtt ccacgtcggg
     2521 catcggattc cag
//
LOCUS       DATDPQ010000319         3262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3045058, whole genome shotgun sequence.
ACCESSION   DATDPQ010000319 DATDPQ010000000
VERSION     DATDPQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045058"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /gene="rpmA"
                     /locus_tag="VI520_07155"
     CDS             <1..262
                     /gene="rpmA"
                     /locus_tag="VI520_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLF37695.1"
                     /translation="KKGGGSTRNGRDSQSQRLGVKRYAGEYLRSGNIIIRQRGSRIHP
                     GLNVDKGGDDTLFATADGVLKFDFGPGGRRRANVIPQTEIVQ"
     gene            259..447
                     /gene="rpmE"
                     /locus_tag="VI520_07160"
                     /pseudo
     CDS             259..447
                     /gene="rpmE"
                     /locus_tag="VI520_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            493..732
                     /locus_tag="VI520_07165"
     CDS             493..732
                     /locus_tag="VI520_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HLF37696.1"
                     /translation="MARTSPSIPISELELGKRAEQALAQAGITQVGQALELMQQGDLA
                     ILAIENFGRKSLADLKRRLKARGFELPTAAKEPQV"
     gene            816..1376
                     /locus_tag="VI520_07170"
     CDS             816..1376
                     /locus_tag="VI520_07170"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HLF37697.1"
                     /translation="MTGSIEVITGSMFSGKTDELIRRLRRATIARQQVQVFKPQLDTR
                     YQVDRVTSHAGSQFEATPVAEADQIPRLVAAESTVVAIDEAQFFDPAIIQVCRELADR
                     GLRVIVAGLDTDFRGDPFGPVPVLMAVGERVDKLQAICMVCGEAASRTQRLIDGRPAR
                     ATDPVIAVGASELYEARCRRHHEVPN"
     gene            1373..1939
                     /gene="hpt"
                     /locus_tag="VI520_07175"
     CDS             1373..1939
                     /gene="hpt"
                     /locus_tag="VI520_07175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HLF37698.1"
                     /translation="MRNYTEFLDEILISEEQLQSRIRELGVEISRDYADLDLLLICVL
                     RGGVLFLADLMRQISVPLSIDFMAVSSYGAGSRQSKGQVRISLDLTTSIEGRNVLLVE
                     DIIDSGHTIASVLEMLSLRRPSSLRVCALLDKAERREVVVPIHYRGFIIPNKFVFGYG
                     LDLDEYHRNLPFVGVLRPDKAPALDQAG"
     gene            1923..>3262
                     /locus_tag="VI520_07180"
     CDS             1923..>3262
                     /locus_tag="VI520_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37699.1"
                     /translation="MTKLAEQLIQPEILTLLRRHAGNREIWLVGGALRDHFLGRSRPD
                     LDFVVDREAAKLARLLSDDLQAGFHSLDDERDAARVILPNLAGTLDFVRRAGASIETD
                     LRERDFTINALALSLVGDERLIDPLGGLQDLRDRRLRPCAKEAVARDPVRALRAVRLA
                     AELGLRMEPETVEQVRAAGDDLAGVSAERVRDEFMRMLEPSIARTAVRLLDHLGLLVR
                     VCPELGQLPGSEHSAREQSLSTLDRLAELVHLLGLRTDRNRDGNLALAEVALQLGKFR
                     AGLSAHLEARLRGGRRVAQLLSLAALYLPSEGSFEPSTASAVSSGVAELALRRGLELR
                     LSRPEAVRIQTIVLNYWRLVKFSTSGPPSDGEVYRFFRASGEAGIELVILYLAASLAL
                     LGSAPDPVEWRSRIAIARTLFQAWFERGELVVRPRKLITGDELATALGLQAGKI"
BASE COUNT          591 a          975 c         1076 g          620 t
ORIGIN      
        1 caagaaaggc ggaggatcca cccgcaacgg ccgagatagc cagtcccagc gcctgggagt
       61 caagcgatac gccggcgaat acctgcgttc tggcaacatc atcatccgcc agcgcggaag
      121 tcggatccat ccggggttga acgtcgacaa aggcggagac gatacactct ttgctacggc
      181 cgatggcgta ttgaagtttg atttcgggcc cggcgggcgc cggcgggcca atgtcattcc
      241 acaaacggag atcgtccagt gagaccaggc attcatccta agtacttccc gaacgcaacg
      301 gtggtctgtt cctgcggcaa cacctggact accgggtcga cggtcgagct gattcacacc
      361 gatgtctgtt ccaactgtca cccgttctat accggcgaac agcggattgt cgacaccgaa
      421 ggccaggttg accgcttcta ccggaagctc gaagcccgcc agggctacct gcaggagatt
      481 cgggagcggg aggtggcacg cacttcgccc tcgatcccga tctccgagtt ggagttgggc
      541 aagcgcgccg agcaggcgct ggcccaggcc gggattaccc aggtcggcca ggcgctagag
      601 ctcatgcagc aaggggacct tgcaatcttg gcaattgaga actttggtcg caagagcctg
      661 gccgacttga agcgccggct caaggcgcga ggtttcgaac tccccactgc ggcaaaggag
      721 ccgcaggtgt agctcgctgg cccattccgg catgaagtag actcaaggca ggtgcacccg
      781 cacctgcctt ttcgttcaga ggagagatta agcggttgac cggttcaatt gaagtcatca
      841 ccgggtcgat gttcagcggg aagaccgatg aattgatccg ccgtctgcgc cgggcgacaa
      901 tcgcccggca gcaagttcaa gtattcaaac cgcagttgga cacccgttat caggtggacc
      961 gggtgacttc tcacgccggg agtcagttcg aggcgactcc ggtggccgaa gcggaccaga
     1021 ttccgaggct ggtggcagcc gagtcaaccg ttgtggcgat tgatgaggcg caattcttcg
     1081 atccggccat cattcaggtg tgccgggaat tggccgatcg aggcttgcgg gtgattgtgg
     1141 ctggcttgga taccgacttc cgcggcgacc cgttcggccc ggtgccggtc ttgatggcgg
     1201 ttggcgaacg ggtggacaag ctgcaggcga tctgcatggt ctgcggggag gccgccagtc
     1261 ggacccaacg gctaattgac ggcaggccgg cccgggccac ggatccggtg atcgcggtgg
     1321 gggccagcga gctatacgaa gcacgctgcc gcaggcatca cgaggtaccg aattgagaaa
     1381 ctataccgag ttcctggatg agatcctgat cagcgaggag caactgcagt cgaggattcg
     1441 agagctggga gtcgagatca gccgggacta tgccgatctc gatctcctgc tcatctgcgt
     1501 gctgcgggga ggggtgctct ttctcgccga cctgatgagg cagatcagcg tcccgctcag
     1561 catcgatttc atggcggtct cctcctacgg tgccggctcc cgccagtcga aaggccaggt
     1621 gcgaatctcc cttgacctga cgacttcgat tgagggtcgc aatgtcctgc ttgtcgaaga
     1681 catcattgac agcgggcaca cgatcgcctc ggttctggag atgctcagcc tccgtcggcc
     1741 gagcagcctg cgggtctgcg ccctgctcga caaggccgag cggcgggaag tcgtcgtgcc
     1801 gatccactat cggggcttca tcatcccgaa caaattcgtc ttcggctacg gcctcgacct
     1861 cgacgaatac catcgaaact tgccgttcgt cggggtcctg cggccggaca aagctcccgc
     1921 gcttgaccaa gctggctgaa cagctcatcc aacctgagat cctgaccctc ttgcgccggc
     1981 acgcagggaa ccgggagatc tggctggtcg gcggcgcgct gcgggaccac ttcttgggtc
     2041 gctcccgacc ggacctcgat ttcgtggtcg atcgcgaggc ggccaaactg gcccgcctgc
     2101 tgtcggacga tttgcaggcc ggcttccatt ccctggacga cgaacgcgat gcggcccgcg
     2161 tcatcctgcc taacctggct ggaactctcg atttcgtccg gagagcaggg gcctcgattg
     2221 aaaccgattt gcgggaacgg gacttcacga tcaacgcgct ggcgctgtcg ctggtgggcg
     2281 acgagcggtt gatcgatccg ctggggggcc tgcaggacct gagggaccgg cgactgcgtc
     2341 cctgtgcgaa agaggcggtc gcccgtgacc cggtccgcgc cctgcgggcc gttcgcttgg
     2401 cggccgagct cgggctgcgc atggaacccg aaacagtcga acaggttcga gccgcgggcg
     2461 acgacctggc gggggtttcg gccgagcgag tgcgggatga gttcatgagg atgctcgagc
     2521 cgtccattgc ccggaccgcg gtccgcttgc tggatcattt ggggcttctc gtccgagtct
     2581 gtccggaact cggtcagctc cccggctccg agcattcggc tcgagaacaa agcctatcga
     2641 cgctagaccg gctagcggaa cttgtgcacc tgttgggtct gcgaaccgac cgcaatcggg
     2701 atgggaacct ggcgttggcg gaggtcgcgc ttcaactcgg aaaattccgt gcgggcctca
     2761 gtgcccacct ggaggcccgg ctgcggggtg ggcggcgggt cgcccaactc ttgagcctgg
     2821 cggcgctcta cctgccaagc gagggtagct tcgagccgag tacggcttcc gccgtctcct
     2881 caggagtggc ggaactcgca cttcgtcgcg gattggagct gcggctcagc cggccggagg
     2941 ctgttcggat tcagaccatc gtactcaact actggaggct ggtgaagttt tcgacctccg
     3001 ggccacccag cgacggcgag gtctatcgtt tcttccgggc gagcggggag gccgggattg
     3061 agctggttat cttgtacctg gcagcctcgc tggccctgct gggctcggct ccagatccgg
     3121 tggagtggag atcaaggatt gcaatcgctc gaacgctctt ccaagcctgg tttgagcgcg
     3181 gtgagctggt agttcgaccg cgaaagctga taaccggcga tgagctcgcc accgccttgg
     3241 gcctgcaggc tgggaagatt at
//
LOCUS       DATDPQ010000320         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3045888, whole genome shotgun sequence.
ACCESSION   DATDPQ010000320 DATDPQ010000000
VERSION     DATDPQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045888"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VI520_07185"
     CDS             <1..622
                     /locus_tag="VI520_07185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HLF37700.1"
                     /translation="INRLFHRGAEVIYDPIAPVHVSGHASQEEMKVMLQLVRPKFFIP
                     IHGELRHLKQHAALAREIGVPDSSIAVVENGRVIEFRDGKMSIGERVPGGYVFVDGTG
                     VGDIGPKVMREREALAKDGFVVIHLTLNHQNGVLARDPEIVSKGFVFVRDAEELFEHA
                     RRRIKELAKKTEPDNLKDEIEKDLTQFFYGETKRHPMVLVFASAQS"
     gene            741..1571
                     /locus_tag="VI520_07190"
     CDS             741..1571
                     /locus_tag="VI520_07190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLF37701.1"
                     /translation="MPEASPLSPTASPQPPTPTALPLAARVNGEGILVEAYVREVARF
                     EASRQALGIELATLGDYRSEVLGTLIDRRIMAQAAERLGIEVTDTELDQRLQSLVELR
                     GGPEGMGAWLAAAGYTADEFENELREEILAARMIQNITSGLSEQADQVHARHILVATE
                     TEAQDLLAQLGAGADFEDLARLFSLDPGTRPAGGDLGWFPQGQLTMPPVEEAAFSLEA
                     GEVSGVIQTVLGYHILQVVEKDRRAVTGEALIRYRVAAVEQWIADQREQAEIEVLVGG
                     "
     gene            1613..2347
                     /locus_tag="VI520_07195"
     CDS             1613..2347
                     /locus_tag="VI520_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37702.1"
                     /translation="MDDPFQRQPPLGGINIGRLVVNLLTGLILVATLLLGTGYVAAFI
                     NPQIPFNPYPPPTLPATLGPPTPTNTPAIYLPTEIPPSATPRPLPTAVPTATETPAVS
                     PTPPTTATVEPVTAEPGAQFQLQPGSPSFTSDERGCAHMGVGGKVYDTIGNPIFNLAI
                     RLGGQLAAVPIGPLDTLTGSAADRFGFGGYYFELSDTPTASDNTLWVQVLDASSGLPL
                     SEQVFLDTFDTCDQNLILVNWKQAGS"
     gene            complement(2349..3521)
                     /locus_tag="VI520_07200"
     CDS             complement(2349..3521)
                     /locus_tag="VI520_07200"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HLF37703.1"
                     /translation="MKIPMSAPDIGESERQAVAAVLHTPRLSMGPQVEAFEASMAHYL
                     GARHAIAASSGTAALHLCVRAAGIRDGDLVITTPFSFVASANVLLYERAIPVFVDVDP
                     RTGNLDPIRVAEAAAGLAGSSAARWLPRLGAERPGELRAILAVDVFGQPADYDRLLAT
                     AQAHGLSLIEDASESLGAHYRGRPAGRFGDAAAFAFYPNKQLTTGEGGMVVTERDDWA
                     ERIQALRNQGRASGDSWLQHTYPGYNYRLDEMSAALGLAQAERLEELLTKRDRVAGWY
                     QEQLDGLPGIELPWIAAETTRMSWFVYVIRLGPGLDRPRLMAELESKGIPSRAYFEPI
                     HWQPYMVERFGFQVGDFPVAEDLGRRSLALPFSGVMPGEQVEYVAAGLREAIERIG"
BASE COUNT          676 a         1164 c         1137 g          652 t
ORIGIN      
        1 gatcaatcgc ctctttcatc ggggggccga agtgatttac gatccgatcg cgccggtcca
       61 cgtctccggg cacgccagtc aggaagagat gaaggtcatg ctgcagctgg tgcggcccaa
      121 gttcttcatc ccgattcacg gtgagctgcg acacctgaag cagcacgctg ccctggctcg
      181 cgagatcggt gtgccggact cgtccatcgc ggtcgttgaa aacggcagag tgatcgagtt
      241 tcgcgatggg aaaatgagca tcggcgagcg ggtgccgggc gggtacgttt ttgtggacgg
      301 gaccggggtg ggcgatatcg gccctaaggt catgcgcgag cgagaagccc tggccaaaga
      361 tgggtttgtc gtgatccacc tgaccctcaa ccatcaaaac ggcgtcttgg cccgtgaccc
      421 ggagatcgtt tcgaaggggt ttgtcttcgt gcgcgacgct gaagaactct tcgagcatgc
      481 ccgccgccga atcaaagagc tggccaagaa gaccgagccg gacaacctca aggacgaaat
      541 cgaaaaggac ctcacccagt tcttctacgg ggagaccaag cgccacccta tggtgttggt
      601 cttcgccagc gcccaatcgt gaggccaacc cgacccgttg tgttgcgtgc ccctcgggca
      661 tgcgtataat ccgccgcgcc atgcggggtc tggtggcgtt atgcctcctc gcaaccgcct
      721 gtggagcccg gacgcccgaa gtgcccgagg cgagcccgct ttcgccaacg gcgagcccgc
      781 agcctccgac cccgaccgcg ctgcccctgg cggcgagagt gaacggggag ggcattctgg
      841 tcgaggccta cgtgcgggaa gtggcacgct tcgaggcctc caggcaggcg cttggcatcg
      901 agcttgcaac ccttggagac taccggtcgg aagtattggg gaccctgatc gaccggcgga
      961 tcatggccca ggccgcggag cggcttggaa tagaagtgac cgacaccgaa ttggatcaac
     1021 ggctgcagtc gctggtcgag ctgcggggtg gtcccgaagg gatgggtgcc tggttggctg
     1081 ctgcgggtta cactgcagat gaattcgaga acgagctgcg cgaggagatc ctggctgccc
     1141 gcatgatcca gaacatcacc tccggattat cggagcaagc ggatcaggta catgcccggc
     1201 atatcctcgt ggcgaccgag accgaagccc aggatttgct ggctcaactg ggggcagggg
     1261 ctgacttcga ggacctggcc cgcttgttct cgctagaccc gggaactcgc ccggccggcg
     1321 gcgacctggg ttggttcccc cagggacagc tcacgatgcc gccggtcgaa gaggccgcct
     1381 tcagcctcga ggccggagag gtcagcggcg tgattcaaac cgttctcggc taccacatct
     1441 tgcaggtcgt cgagaaggac cgccgggcag tgaccggtga ggcgctcatt cgttaccggg
     1501 tcgcggcggt cgagcagtgg atcgcggacc agcgggagca ggctgaaatc gaggtactgg
     1561 tcggtggatg agattagttc tggcggccta acgtctggta gaggcaatgc gcatggatga
     1621 tccttttcag agacagcccc cgctgggggg gataaacatc ggtcggttgg ttgtgaacct
     1681 cctgaccggc ctgatattgg tggcgaccct gctcctcggc actgggtacg tcgctgcatt
     1741 catcaatcct cagatcccat tcaatccgta tccaccgccg acattgccgg cgacccttgg
     1801 tccgcctact ccgaccaaca ccccggcgat ctacctgccg acggagatac caccctctgc
     1861 cactccccgc ccgctcccga ccgcggtccc gaccgcaacc gagacgccgg cagtgagccc
     1921 aacccctcca acgacggcta cggtggagcc cgtcaccgcc gagccggggg cgcagtttca
     1981 gctgcagccc ggcagcccgt ctttcacttc cgacgagcgc ggttgcgccc acatgggcgt
     2041 cgggggcaag gtgtatgaca ccatcggaaa tccgattttc aacctggcga ttcgactggg
     2101 tggtcagctg gctgccgtgc cgatcgggcc gctggacacc ctcaccggga gtgccgcgga
     2161 ccgcttcggg ttcggcggct actactttga attgtcagac acgccgactg ccagcgacaa
     2221 caccctgtgg gtccaggtac tggacgccag cagcggattg ccgctctcgg aacaagtctt
     2281 cctcgacacc ttcgatactt gtgaccagaa cttgatcctg gtgaattgga aacaggcggg
     2341 gtcctagcct agccgatccg ctcgattgcc tcacgcaacc cggcggccac atactcaacc
     2401 tgctcgcctg gcatcacccc cgaaaagggg agtgccaggc tgcgccgtcc aagatcctcg
     2461 gccactggaa aatcaccaac ctgaaacccg aatcgctcca ccatgtacgg ctgccagtgg
     2521 atgggttcga aataggcccg gcttgggatt ccctttgatt caagttcagc catcaaacgg
     2581 ggccggtcga ggcccgggcc gagtcgaatg acgtaaacga accagctcat ccgagttgtc
     2641 tctgccgcga tccagggaag ctcgatcccc ggtagcccgt ccagttgctc ctggtaccag
     2701 ccggcaaccc gatctcgttt cgtcagcagc tcctctaacc gctcggcctg ggccaggcca
     2761 agggccgcgc tcatctcatc caagcggtag ttgtagccgg gataggtatg ctgcaaccag
     2821 ctgtcgccgg aagcgcgacc ttgattacgc aatgcttgga tgcgttcggc ccagtcgtct
     2881 cgctcggtaa ccaccatccc gccttccccg gtcgtcaact gtttgttggg atagaaagca
     2941 aaggcggccg catccccgaa ccggccggct ggccggccac ggtagtgagc accgagggac
     3001 tccgaggcgt cttcaatcag gctcagccca tgggcctgcg cggtggcaag cagccggtcg
     3061 tagtcggccg gctgaccgaa cacatctacc gccagtatcg cccgcagctc acccggtcgt
     3121 tccgcaccga ggcggggtag ccaccgggca gctgacgacc cggcgagtcc tgcggccgct
     3181 tcggccaccc ggatgggatc caggttgccg gttcgtggat ccacatccac aaacaccggg
     3241 atggctcgct cgtacagcag cacattcgcc gaggcgacaa acgagaacgg tgtggtgatc
     3301 accaggtccc catcccggat gccggcggct cgcacgcaca ggtgcagggc ggcggtcccc
     3361 gaactggctg cgatcgcatg cctcgccccg agatagtgcg ccatagacgc ttcaaaagcc
     3421 tccacctggg gccccatgct gagccggggc gtgtggagca ctgctgcgac ggcctggcgc
     3481 tcagattcac caatatcggg cgcggacatc gggattttca tcgaacgagc ctgagctcaa
     3541 gcccgagcgc ctcgggcagc gctcgcaggt cgcttatttc ctgatcggga gcatagtttg
     3601 gaccgatcgg ctccttgtgc ccatgctct
//
LOCUS       DATDPQ010000321         4243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2531907, whole genome shotgun sequence.
ACCESSION   DATDPQ010000321 DATDPQ010000000
VERSION     DATDPQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531907"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VI520_07205"
     CDS             complement(<1..1551)
                     /locus_tag="VI520_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLF37704.1"
                     /translation="MATMAATAEVFTRKASGLVRVMSPYSAFIYNILTMGLIFPWTYL
                     WAPGALPGGNMVWGILLAMLFQIPVALSIVWLSTALPRSGGDYVFQSRVLGGGLGFTA
                     VMSGFVIWILQWVALSGWLLAYLGFAPLFLGLGATLGSSGLSNAGVWFTTGTGIIVTS
                     IANAALSAGILVSGFKNYVRFQYVMFYGTLAAFAVMFVVLFTTPSGEFASRLNDFAVA
                     SGAAPNFYQTAVDAVRNAGIDLRPPFSLLATLLVAPIAWTSLQWSTYSAEQNGEIKEA
                     RSFRNQAIIMVGSLIVTGLLLALLAVGLQRLAGSEFLYVAGAGYWSLIPEATVNGFYL
                     WPNILAVAMTASPLIVLLIGIGYILNSHQIVHNCYIGVTRIMVAMSLDRVLPEWVSKV
                     HDRLHTPVNAHWAYFLASVPVILAYNLVGGWVGLTLGVTFGGGYVFVLSCLSGALLPK
                     RAKAVYDASPGAKYTVSGLLGVLFIVVGAAAFIWASWVFAGNFPIGAIGWLIRLGAVA
                     AVFWILSSF"
     gene            complement(1709..2704)
                     /locus_tag="VI520_07210"
     CDS             complement(1709..2704)
                     /locus_tag="VI520_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37705.1"
                     /translation="MSLFTRKPARKTTRLFFATDVHGSDRTFRKFINSAKFYEADVLV
                     MGGDILGKLAIPIIQEGDGRYRATLQGRTETIEGDSGLERLRSRLGTLGFYSKVMNED
                     EFRSIQAEPAAVEDLFKELARQRLEAWVELAETRLAGSGVRCFVTGGNDDYSEVLEVL
                     YRGGTQAIFGCEGKLVNLDDDHTLISVGYSTPTPWHTPREVSDDQLGEMIEAAVQPAS
                     NLSHSIFNFHDPPVDSSLDTCPMLDWSTDPPTQIVKSGQVVLFGAGSQSVRRAIEAHQ
                     PVLGLHGHIHESPGVINIGRTLCVNPGSEYGEGILRGVLVSLADGEVTGHQLTSG"
     gene            complement(2729..3541)
                     /locus_tag="VI520_07215"
     CDS             complement(2729..3541)
                     /locus_tag="VI520_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HLF37706.1"
                     /translation="MQVSEPLIPAQRRHRIREYLQRHQIVRSATLSEILGVSEATVRR
                     DLVWLEQQGEVERTHGGAVLTQRLPTEPSYASSVLAHPEEKRLIGQTAATLVEPGDTI
                     FVNSGTTASEVLRHLRARGLTDLTVVTNNVAAALESREPGFELILLGGSFRPSSNSVS
                     GRFAADLIRQVYADKAFIGVDGISLHHGCTTPINTEAEVAGLMIERTQGPVIIVADHS
                     KWGVVSNFEVTPLSKVNTLVTDEGLAPEWRTELRARSIEVRIGAANGPDGRR"
     gene            3677..>4243
                     /locus_tag="VI520_07220"
     CDS             3677..>4243
                     /locus_tag="VI520_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37707.1"
                     /translation="MSSSTIGMGDQGQEERQRFEAEVRRVVEASASGGVTLRLLGSLA
                     FHAHCPRYGYLQAALGRAYTDLDFGGYVRQAKEVGGILTGLGYREDREVFVVTEGGRA
                     IFEHPGNGLHVDVFYEKLDFCHVIPWQGRLELDSPTLPLAELLLEKMQIVEINEKDVI
                     DTIMLLLEHPLGGGDAETINLDRIAALCA"
BASE COUNT          819 a         1351 c         1362 g          711 t
ORIGIN      
        1 gaaactggaa agaatccaga agacagcagc gaccgcccca agccggatca gccagccgat
       61 cgctccgatt gggaagttcc cggcgaagac ccaggaagcc cagatgaacg ccgccgcccc
      121 gacgacgatg aacaacacgc cgagcaaccc ggatacggtg tactttgcgc cgggtgaggc
      181 gtcataaacc gccttagccc gcttcggcag cagcgctccc gagaggcagc tcagcacgaa
      241 cacatagccg ccgccgaagg tgacgcccag ggtcagcccc acccagccac caaccaggtt
      301 gtaggccagg atcactggga ccgaagccag gaagtacgcc cagtgggcgt tgaccggagt
      361 gtgcagccgg tcgtggacct tgctcaccca ctccggcagc acccgatcga gcgacatggc
      421 caccatgatc cgggtgacgc cgatgtagca gttgtgcacg atctggtggg agttcaggat
      481 gtatccgatt ccgatcagca gcacgatcag cgggctggcg gtcatcgcca cggccaggat
      541 gttcggccag aggtagaagc cgttcaccgt ggcctccgga atgagcgacc agtacccggc
      601 gccagccacg tacaggaact ccgagccggc cagccgctgc agtcctaccg cgagcagcgc
      661 cagcagcagg ccggtcacga tcagcgagcc gaccatgatg atcgcctggt tgcggaatga
      721 gcgagcttcc ttgatctcgc cattctgttc tgcagagtag gttgaccact gcagggaggt
      781 ccaggcgatc ggggccacga gcagcgtcgc cagcaggctg aagggcgggc gcaggtcgat
      841 cccggcattc cgcaccgcgt cgaccgcggt ctgatagaag ttcggggcag ctccggaggc
      901 gaccgcaaag tcgttcagcc ggctcgcgaa ctcacccgac ggtgtggtga acagcacgac
      961 gaacatcacc gcgaacgcgg ccagcgtccc atagaacatc acgtactgga agcggacgta
     1021 gttcttgaag ccgctgacca ggatccccgc ggagagcgcc gcgttggcga tgctcgtgac
     1081 gatgatgccc gttccggtgg tgaaccacac cccggcgttg gacaggcccg agctgcccag
     1141 cgtggctccc agaccaagga acaacggggc aaacccgagg taggcgagca gccagcccga
     1201 cagggcgacc cactgcaaga tccagataac gaatccggac atcacggcgg tgaagcccaa
     1261 tcccccgccg agcacacggc tctggaagac atagtctccg ccggagcggg gcagcgcggt
     1321 cgacagccag acgatgctta aggcgaccgg gatttggaag agcatcgcca gcaggatccc
     1381 ccacaccatg ttgccgcctg gaagggcccc cggggcccag aggtaggtcc aggggaagat
     1441 caaccccatc gtaaggatgt tgtagatgaa cgccgagtag ggagacatca cccgcaccag
     1501 gccggacgcc ttgcgggtga agacctcggc ggttgcagcc atcgtagcca tattacgttc
     1561 ctcctcctga atgattcaac gcggaccgaa ggaatttagc acacggactg ctcgatagaa
     1621 ccctcctcac tcctcagctt caccacctcc gcctcgcccg cattgcctct gcgaaaggac
     1681 acgctgggac aggcagagcg gcgtgaggtt acccggaggt cagctgatgg ccagtgacct
     1741 ctccgtcggc aagcgaaacc agaaccccgc gcagaatgcc ctccccatac tcgctgcccg
     1801 ggttgacgca caacgtccga ccgatattga tgactcccgg cgattcgtgg atgtgaccgt
     1861 gcagcccaag cactggttga tgggcctcga tcgcccggcg cacggactgg ctgcccgctc
     1921 cgaagaggac tacctggccg ctcttcacga tctgggtcgg gggatcggtg ctccagtcca
     1981 gcatcggaca ggtatccagg ctggagtcga ccggcgggtc gtgaaaattg aaaatgctgt
     2041 ggctcagatt ggaggccggc tgcaccgcgg cctcgatcat ttcgcccagc tggtcatccg
     2101 agacctcccg cggcgtgtgc cacggagtgg gggtgctgta gccgacgctg atcagggtgt
     2161 ggtcgtcatc gaggttcacc agcttgccct cgcagccaaa gattgcctgc gtcccgccgc
     2221 ggtagagcac ttcgagcact tcggaatagt cgtcatttcc gccggtaaca aagcagcgca
     2281 ctccggaacc ggccagccga gtctctgcca gctcgaccca ggcctcgagc cgctggcggg
     2341 ccagttcctt gaagagatcc tcgaccgcag ccggttcggc ctggatgctg cggaattcgt
     2401 cctcattcat caccttactg tagaacccca gcgtccccag acgggagcgg agccgctcga
     2461 ggccagaatc gccctcgatc gtctcggtgc gcccttgcag cgtggcgcga tagcggccgt
     2521 cgccctcctg gatgatcggg atggccagct tgccgagaat gtcgccgccc ataaccagca
     2581 cgtcggcctc gtagaacttg gcggaattga tgaatttgcg aaaggttcgg tcggagccgt
     2641 ggacgtcggt ggcgaagaag agccgggtgg tcttgcgggc gggcttgcgg gtgaacagcg
     2701 acatgccgtc tctgcctcag ggccccgcct acctccgccc gtccggcccg ttggccgccc
     2761 cgatccgcac ctcgatcgag cgggcccgga gctcggtacg ccattcgggc gcgagtccct
     2821 cgtcggtcac cagggtgttg accttggaca gcggggtcac ctcgaaattg gacaccactc
     2881 cccacttgct gtgatcggcg acgatgatca ccggtccctg agtgcgctcg atcatcaagc
     2941 cggcgacttc ggcctcggtg ttgatcggag tggtgcaccc gtggtgcaga ctgatcccat
     3001 cgacgccgat gaatgccttg tcggcgtaaa cctggcggat gaggtcggcc gcgaagcggc
     3061 cggataccga attggagctg ggccgaaacg atccgccgag caggatgagc tcaaatcccg
     3121 gctcacggga ttccagcgcg gcggctacgt tgttggtgac gacggtgaga tcggtcagcc
     3181 cgcgagcgcg caggtggcgc agaacttcgg aagccgtcgt tccgctgttc acgaagatcg
     3241 tgtcgcccgg ctcgaccagg gtggcagccg tctgcccgat caggcgtttc tcctcggggt
     3301 gcgccaggac actggaggca taggacggct cggtaggcaa gcgctgggtg agcacggcgc
     3361 cgccgtgggt ccgttcgacc tcgccctgct gctccaacca gaccaaatcg cggcggaccg
     3421 ttgcttccga aactcccagg atttcactca acgttgcgct gcgcacgatt tgatggcgct
     3481 ggaggtactc tcggatcctg tggcgccgct gtgccgggat gagcggctcg ctgacctgca
     3541 ttcgaccctg cagtgacgga aattgagcgg tcttgtgcgg ttggggcccc ttgattatga
     3601 tcgattctga tcagttctgc aaggctgaag agggagggtt agaatcgcgg ccaggcactc
     3661 acacggcgaa cccagcatga gctcgtcaac catcggaatg ggcgaccagg gccaagagga
     3721 gcggcagcgc ttcgaggcag aagttcgccg cgtcgttgag gccagcgcca gcggcggagt
     3781 gaccctgcgc ctgctcggtt ccctggcctt ccacgcccac tgcccccgct acggttacct
     3841 gcaggcggcc ctgggccggg cctacaccga ccttgacttc ggtgggtatg ttcgccaggc
     3901 gaaggaggtc ggggggatcc tgaccgggct tggctaccgg gaggaccggg aggtcttcgt
     3961 tgtcacggaa ggcgggcggg ccattttcga gcatcccggc aatggactgc acgtcgacgt
     4021 cttttacgaa aaactcgact tctgccatgt cattccctgg caagggcggc tggagctgga
     4081 ctcgccgacc ctgcccctgg ccgagctgtt gcttgagaag atgcagatcg ttgagatcaa
     4141 tgagaaggat gtcatcgaca cgatcatgct gctgctcgaa cacccccttg gcggcggcga
     4201 cgccgaaacg atcaacctgg accggatcgc cgcgctgtgt gca
//
LOCUS       DATDPQ010000322         2833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1498101, whole genome shotgun sequence.
ACCESSION   DATDPQ010000322 DATDPQ010000000
VERSION     DATDPQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498101"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VI520_07225"
     CDS             complement(<1..410)
                     /locus_tag="VI520_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37708.1"
                     /translation="MTRLFFATDVHGSDVCWRKFLNAAKFYRADVLVLGGDMTGKALV
                     PITQFPDGTYKATLLQQEFILHTEDAVRDMERRVGSRGYYPFRVTLEQLAELSADPAK
                     VDAFFLDRTLTLLDQWMALADERLNGTGVRCYVCP"
     gene            complement(649..951)
                     /locus_tag="VI520_07230"
     CDS             complement(649..951)
                     /locus_tag="VI520_07230"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HLF37709.1"
                     /translation="MSSDYQIVYWRDIPAQVKIRHDGGRAARPLSNRFQAAIDEAAMH
                     SGATDTDSYLEQWRSSDWQVRDGEPKALAAALVSELEAAYPPERLIALTANGGREG"
     gene            complement(948..1532)
                     /locus_tag="VI520_07235"
     CDS             complement(948..1532)
                     /locus_tag="VI520_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HLF37710.1"
                     /translation="MTRTALIVCGALAREVLALRDKHGWEADVLGVPALLHNRPDRIP
                     DAVLARIREARAAYDRVLVVYGDCGTGGLLDRALEREGVERLAGPHCYEMYADHKFDE
                     LMAEAPGTFFLTDYLVRSFDHLVIEGLGLDRHPELRDDYFHNYTRVIYLAQQADPALV
                     TQAQQAAARLGLPLTIGQVGYGALERRLQAWFER"
     gene            complement(1529..2464)
                     /locus_tag="VI520_07240"
     CDS             complement(1529..2464)
                     /locus_tag="VI520_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLF37711.1"
                     /translation="MDRQAWLRILDEAGGVLLADGAMGTMLFDSGLEFGEAPERWNLE
                     HPDRVGAVHAGYLAAGSQVLLTNTFGANRFRLALHGLEDRVAELNRAGAETLHAAVAA
                     AGRPALVAGDIGPSGSLLTPLGDLEFGDAVAGFAEQAGALAAAGVDLLWIETMSDLDE
                     IRAALEGVRQVAPEMAVVTTMSFDTHGRTMMGVKPEDAVQALLSWGATAVGGNCGNGP
                     EEVIQVVHKMRAVAPETVLVAKGNAGMPELVKGRACYHATPDSMADYLRRAVGAGAAV
                     VGACCGSTPEHVRAMAGALNEIRRQRVLSPAEGPV"
     gene            complement(2464..>2833)
                     /locus_tag="VI520_07245"
     CDS             complement(2464..>2833)
                     /locus_tag="VI520_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HLF37712.1"
                     /translation="KGDIHDIGKNLVGMMMEGAGFKVVNLGVNNDVTKFMAAIEEHKP
                     DILGMSALLTTTMPYMGIVIKALKEAGIRDQLIVLVGGAPLNAAFAEEIEADAYCRDA
                     AIAVVTAKQLMAIKRGEVAL"
BASE COUNT          497 a          979 c          859 g          498 t
ORIGIN      
        1 ggacaaacgt agcagcgcac gccggtccca ttcagccgct catcggctag ggccatccac
       61 tgatccagca gggtcagagt ccggtcgagg aagaaggcat ccactttggc cggatcggcc
      121 gagagttccg ccagctgctc gagagtgact cggaatgggt aatagccgcg gcttccgacc
      181 cgccgctcca tatcccgaac ggcgtcctcg gtgtgcagaa tgaattcttg ctgcagcagg
      241 gtggctttgt aggtcccgtc ggggaattga gtgatcggaa ccagcgcctt gccggtcatg
      301 tcaccgccaa ggaccaacac gtcggcccgg tagaacttgg ccgcgttgag gaacttgcgc
      361 cagcagacgt cggaaccatg gacgtcggtg gcgaagaaaa ggcgcgtcac caggcctccc
      421 cacaggcccc cgccggcggg gagccacgac tcgcccttcc ctcggtttca agcgtaattc
      481 gattccggcg aatggtcaat cggagaagct ggaggcggcc aatcaaatcg aatggcgggt
      541 ctggagtttg gctaccgctg gcgggctacg cctggcggga gggcgggctc attgaccgaa
      601 ctcggtcacg gtcggtattc ttgcggagcc gtccgccagc ggtcggattc agccttctcg
      661 gccgccgttg gcggtgagag cgatcaagcg ctcgggaggg taggcggctt cgagttcgga
      721 gaccaaagcc gcagccaggg ctttgggctc cccatctcgg acctgccagt cggagctgcg
      781 ccactgctcc aggtagctgt cggtatcggt cgccccactg tgcatcgccg cttcgtcgat
      841 agctgcctga aatcggttgc tcagcggccg ggccgcccgg ccaccatcgt ggcggatctt
      901 gacctgggcc gggatgtccc gccaatagac gatctggtag tcagaactca tcgctcgaac
      961 caggcctgca gccggcgttc cagcgcgccg taaccgacct gcccgatcgt gagcggaagc
     1021 cccaggcggg ccgcagcttg ctgcgcctgg gtcacgaggg cggggtcagc ttgctgggcc
     1081 aggtagatca cccgggtgta gttgtggaaa tagtcgtcgc gcagctcggg atgacggtcg
     1141 agccccaggc cttcgatcac cagatgatcg aaagaacgca ccaggtaatc ggtgaggaag
     1201 aaggtgccgg gagcctccgc catcagctca tcaaacttat gatcggcgta catctcatag
     1261 cagtgcgggc cggccaaccg ctccacgccc tctcgctcca gagcacgatc gagcagaccg
     1321 cccgttccgc agtctccata cacgaccagc acccggtcgt acgcggcgcg ggcttcccga
     1381 atccgcgcca gcaccgcgtc cggaatccga tccggccggt tatgcagcag cgccggaaca
     1441 cccaggacgt cggcctccca cccgtgtttg tcccgcagag ccaggacctc tcgggcgagg
     1501 gcaccgcaca cgatcaatgc ggttcgggtc aaactggacc ctccgccggg gagagcaccc
     1561 gctggcggcg gatttcgttc aaggcaccgg ccatcgcccg cacatgctcc ggcgtactgc
     1621 cacagcaggc gcccaccacc gccgcgccgg cccccaccgc tcgcctcaga tagtccgcca
     1681 tgctatccgg tgtggcatgg tagcaggcgc gtcccttgac cagctccggc attcccgcat
     1741 tgcccttagc gaccagcacc gtttcgggag cgacggcccg catcttgtgg acaacttgga
     1801 tcacctcttc cgggccattg ccgcaattcc cacccaccgc ggtggctccc cacgagagga
     1861 gggcctgcac tgcatcttcc ggcttcactc ccatcatggt tcgaccgtgg gtatcgaaac
     1921 tcatcgtggt cacgaccgcc atctcaggcg ccacctgccg aacgccctcc agtgccgccc
     1981 ggatttcatc gagatccgac atggtctcga tccagagtag atcgactccc gcagcagcca
     2041 gcgctccggc ctgttcggcg aagccggcca ctgcgtcccc gaactctaga tcaccgagcg
     2101 gtgtgagcaa agatccgctc gggccgatat ctccggcaac cagcgcgggc cgcccggccg
     2161 cagcgaccgc ggcatgcagg gtctcggccc ccgcccggtt gagctcggcg acccgatcct
     2221 ccagcccatg cagcgccagc cgaaaacggt tggccccgaa ggtgttggtc agcagcacct
     2281 gcgagccggc cgccaaatag ccggcgtgca cggcacctac ccggtccggg tgctcgaggt
     2341 tccagcgctc gggagcctcg ccgaactcca ggccggagtc aaacagcatg gttcccatcg
     2401 cgccatcggc caacaggacg ccccccgctt cgtccagtat tcgcagccag gcttgtcggt
     2461 ccattacaga gccacctcgc cgcgcttgat cgccatcaat tgcttggcgg tgaccaccgc
     2521 gatcgccgcg tcgcggcaat aggcgtccgc ctcaatctct tcggcaaacg cggcgttgag
     2581 tggcgcgccg ccgaccagca cgattagttg gtcgcggatc cccgcctcct tcagtgcctt
     2641 gatcacgatc cccatgtagg gcatggtcgt ggtaagcagc gcactcatcc ctaggatgtc
     2701 cggtttgtgt tcttcgatgg ctgccatgaa cttggtgacg tcgttgttca ccccgaggtt
     2761 gaccaccttg aatcccgccc cctccatcat cattcctacc aggttcttgc cgatatcgtg
     2821 gatgtcgccc ttc
//
LOCUS       DATDPQ010000323         8962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1755584, whole genome shotgun sequence.
ACCESSION   DATDPQ010000323 DATDPQ010000000
VERSION     DATDPQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755584"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /gene="tmk"
                     /locus_tag="VI520_07250"
     CDS             complement(<1..218)
                     /gene="tmk"
                     /locus_tag="VI520_07250"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLF37713.1"
                     /translation="MSFFITIEGPDGSGKSTQAQLLADHLKDKGYDVLFTREPGGTEI
                     GDQIRRVIMSLDNKRMSPQAEFLLFSASR"
     gene            complement(215..1810)
                     /locus_tag="VI520_07255"
     CDS             complement(215..1810)
                     /locus_tag="VI520_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HLF37714.1"
                     /translation="MTGKRITDDLDALRSVLPSHITDKLAQINQPDDLLEVILDIGRV
                     PTARYVKEEVTLSDKEVTWDDLDSVVDRIGEFDADNRAGLERTLHRISAIRNRHDRVV
                     GLTCRVGRAVYGTIDIIQDLISNGKSLLLLGRPGVGKTTMLREVARILAEERRVVIVD
                     TSNEIAGDGDVPHPAIGRARRMQVQKPSLQHEVMIEAVENHNPEVIVIDEIGRELEAV
                     AARTIAERGVQLIGTAHGNTLENLLLNPTLSDLIGGIESVTLGDEEARRRGTQKTVLE
                     RRAAPTFDVLIEIQDRERLAVHHDVAAAVDALLRGRPLPAESRHRDESGEIKIEKATV
                     LSGSDRRSAAGYRRQSALSSTRPEEPAELPFSPAAPHRPGDPLDTIRIYPHGVARNRL
                     RQAAKRLHVPALQVDDFTQADVLVTLRSYYRRQHRFIADAEARRMPIYVLRANTVGQM
                     ENFLVELFSLDREPAGDPSEDQATQEAQEAIQAVLNGARSVDLKPTTAYGRRLQHQLA
                     RQANLISHSYGREPQRRVRIFRD"
     gene            complement(1807..2748)
                     /locus_tag="VI520_07260"
     CDS             complement(1807..2748)
                     /locus_tag="VI520_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37715.1"
                     /translation="MIRELDWRDLPLLHRVRDRGICPDSQLAFTRGANAFQNALLDVF
                     IPGRSAITLVAREGDHDAVGQCIHRGDSPVARITFLGPIEALQHDLGMQLVESLAFTA
                     GEHGAFNLVADVDEHSFAFDRLRQAGFAVYARQRIWRLGNDRQDLPDPQGIAWQLESS
                     SDSLAAQSLYLNIVPALIQQVEAGPSSSGHNLVHWRDEELLGYLDIERGSLGTWVQPF
                     FHPAAEDFYRLMANFLHQTPVDRPLFVCVRSYQSWMGRPLERLWFEPEVDQAVLVKRL
                     TARVRKPVKGRLRTVEGTYPEPTAPIARIEEPAGAPR"
     gene            2857..3459
                     /locus_tag="VI520_07265"
     CDS             2857..3459
                     /locus_tag="VI520_07265"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HLF37716.1"
                     /translation="MGTPPGVERAPRYNQSDCCAAEIAMNARRVSLSAVSIALVFVLI
                     RSIQVPIVATEGFTHPGAVAEIFVSLAFGPSIGLVAAAGGAALADLTSGQFAKFAPLT
                     LLAHGTLGFLAGYLGRKKQWPNMLAGWLVGGIALVGIYFVGESLVANLYGGYAAALIE
                     LPFNLVQVGLGIFGIFLYRVTRAAYPRIDQLTEHPRFEEV"
     gene            3560..4426
                     /locus_tag="VI520_07270"
     CDS             3560..4426
                     /locus_tag="VI520_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37717.1"
                     /translation="MTTAVVIQDLRYAYPNGAETHEASWVLRGVDMVVDEGEFLSIVG
                     PTGSGKSTLCLALNGVVPNTTGGRMRGDVSVFGVNTKKHPVAALAKLVGVVFQDPETQ
                     LFNMDAEAEVAFGLETMGMPSEEIGERVDWALALMGLREMRDRSPLQLSGGEKQRLAI
                     ASVLAMTPKVLVLDEPTNNLDPQGKRDLFTALWDLRQSLGLTVIMVEQETEFVAEFSD
                     RVALMRDGHLEIVGPPATVLGDVERMREIGVAIPQVTEVAYCLNRDFGLGLAFTRFGE
                     ALLSLEAKLHES"
     gene            4416..5297
                     /locus_tag="VI520_07275"
     CDS             4416..5297
                     /locus_tag="VI520_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37718.1"
                     /translation="MKAEKDPSRFAAVCLEIRDLWFTYGDGQDVLNGISLSIAEGDFV
                     AVIGQNGSGKTTLAKHFLGLLRPTKGEILLHGERIRDRSVGELARQVGYVFQNPDHQI
                     FGTTTWEEIAFGPRNLGLSAEEVERRTVDALERFDLSSEATKPPAMLSFGQRRKVSLA
                     AVYAMDPHVLILDEPTNGLDGRGEKEAMELARTLNNTGKTIILVTHRIRLVAEYARSC
                     ILLAGGRVSAYGDTRSVFEQDDLLKSSNIELPQVWELGRQLAPRGFLQPALSVGDFCQ
                     TYGELLKQTGFRNAGAR"
     gene            5281..6090
                     /locus_tag="VI520_07280"
     CDS             5281..6090
                     /locus_tag="VI520_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HLF37719.1"
                     /translation="MPVLADFFMRGDSWLHKTDPRVKVLSVGLTLAAALILQNLLMMV
                     ILWLLVLAVPFAARAPRGRIRFVLSALVPVSVLMPLVWMVFYPIGVETISFWFIEVKL
                     LSLVQGLTVVLRIHIVSFSVFALLFSTDQTTLVRGLVKLRLPYVWGLVLSLALRYIPT
                     FERSFADILQAQKARGLEFESMRGWRRVRALMPVFVPTVISLFRLSEQIGIALEARGF
                     GAEGVRRTYFRDITFRTLDWLYIAIVVTAVGSLFYFNLKYGFGADPLRLVR"
     gene            6097..7131
                     /locus_tag="VI520_07285"
     CDS             6097..7131
                     /locus_tag="VI520_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HLF37720.1"
                     /translation="MVRPSEPFPLGKIPNPVLRELLAGLGSTDPRVRVGPGIGLDAAV
                     IDLGDRYLVAKTDPITFASDWVGWYAVHVNANDVACMGAEPRWFLASLLLPEGKTDRG
                     LAETIFSQIGEACHSLEISLVGGHTEISHGIDRPIVVGCLLGEVAKDGLVTAAGAQAG
                     DVLLLVGGIPIEATAILAREKAGELEGRFAPQFLERCRKFLFEPGISVVRAARLAVTA
                     GQIHAMHDLTEGGLLAGLWELAEASGCGLEVDREAILILPEGQALCEAFGLDPLASIA
                     SGAFLLTAPHDRQPAIEQALSAQGIVVSAIGQVISRAGSEVRLRSAGGIGLAPKPERD
                     EIARLLQRSV"
     gene            7341..7730
                     /locus_tag="VI520_07290"
     CDS             7341..7730
                     /locus_tag="VI520_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLF37721.1"
                     /translation="MVALDFRSKEPIYRQIMGQIQEQLASGELKPGQQLPTVRKLATQ
                     LGVNFNTVARAYRLLDQQGVISTQHGRGTYALGRRSVARPRRTRLERLAQGFAREAAR
                     LGFRPEQVAETIGYVLEEWDQRGRPVG"
     gene            7791..8630
                     /locus_tag="VI520_07295"
     CDS             7791..8630
                     /locus_tag="VI520_07295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HLF37722.1"
                     /translation="MNGLLMLAMPLGLGVVLSRRTGRRWGLYGAGALTFIGSQVLHLP
                     FNGLVLNPQLAALGFGERGLSGTLVLSAVLLGLSSGVFEEGARFLVLRFWQRSARSWR
                     DSLMFGAGHGGTEAILVGALALVAFFQALAYRNTDLSQILPADQVATAASQVEAYWTA
                     PWPFALLGAAERAFSLAAHLSLSAMVMRAFTHRNAGWLVAAIGWHALINTLAVIALFT
                     VSPVATEGLIGLTAGVSVGILFALREPPVVVSAEPGEQLARVTAAKASLPLARVEDSK
                     YAE"
     gene            8669..>8962
                     /locus_tag="VI520_07300"
     CDS             8669..>8962
                     /locus_tag="VI520_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF37723.1"
                     /translation="MIRIEALSKRFGSTVAVENLWLEIAPGELFGFLGPNGAGKTTTI
                     RMLTGLISPSSGRAWVAGHALGEDDTELRRQVGILTEAPGLYDTLSAERNLAFF"
BASE COUNT         1625 a         2569 c         2931 g         1837 t
ORIGIN      
        1 cgggaggccg agaagagcag aaactctgct tggggactca tccgcttgtt gtccagcgac
       61 atgatcaccc ggcgaatctg gtcgccgatc tcggtcccgc cgggctcgcg ggtaaacagg
      121 acgtcgtacc ctttatcctt gagatgatcg gccagcagtt gggcctgggt ggacttaccg
      181 ctgccgtccg gcccctcgat ggtgatgaag aagctcaatc tcgaaagatc cggacccgcc
      241 gctgcggttc acgcccgtag gagtgtgaga tcaagttggc ctggcgggcc aattggtgct
      301 gcagccggcg cccgtaggcc gtggtcggct tgaggtcgac cgaacgggcg ccattcagta
      361 cggcctggat tgcttcttgg gcctcctggg tggcctggtc ctctgagggg tcgccggccg
      421 gttcgcgatc gaggctaaag agctcgacca gaaaattctc catctgaccg accgtgttgg
      481 cccgcaagac atagatcggc atccggcgcg cctcggcgtc ggcgatgaag cggtgctggc
      541 ggcggtagta ggagcgcaaa gtgaccagca catcagcctg ggtgaagtcg tccacctgca
      601 gggccggcac atgcagacgc ttggcggcct gccgcagccg attgcgggcc accccgtgtg
      661 ggtagatccg gatcgtgtcc agcggatcgc cgggtcgatg cggtgcggcc ggcgagaacg
      721 gcagctcggc cggctcctcc gggcgggtgc tggacagggc cgattgccgc cggtagccgg
      781 ctgcgctccg gcgatcggaa ccggacagaa cggtggcttt ctcgatcttg atctcgcccg
      841 attcatctcg gtgacgggat tccgccggca gcggccggcc gcgcagcaat gcatccactg
      901 cggcggccac gtcgtgatga accgccagcc gctcccggtc ctggatctcg atcaagacat
      961 cgaaagtcgg agccgcgcgc cgctcgagga cagtcttctg ggtcccgcgc cggcgagcct
     1021 cctcatctcc aagggtgacc gattcgattc ctccgatcag atccgagagg gtggggttca
     1081 gcagcaagtt ctcgagggtg ttgccatggg cggtgccgat gagctgcacc ccccgctcgg
     1141 caatcgttcg ggccgccacc gcctcaagct cgcgcccaat ttcgtcgatg acgatcactt
     1201 ccggattgtg attctcgact gcctcaatca tcacctcgtg ctgtaggctc ggcttctgaa
     1261 cctgcatccg gcgggcacgc ccgatggccg gatgcggcac gtctccgtcg ccggcgattt
     1321 cgttggaggt gtccacgatc actacccggc gctcctcggc caggatccgg gccacttccc
     1381 gcaacatcgt ggtcttgccc actcccggtc gcccgagcag cagcaggctc ttcccgttgc
     1441 tgatcaggtc ctggatgatg tcgatcgtgc catataccgc tcggccaact cggcaggtca
     1501 gcccgactac ccggtcgtgg cggttgcgga tggcggagat ccggtgcagg gtgcgttcga
     1561 gcccggcccg attatcggcg tcgaactctc cgatccggtc gacgaccgaa tcgaggtcgt
     1621 cccaggtcac ctctttgtcg ctaagggtga cctcctcctt aacgtaacgg gcggtaggga
     1681 cccggccgat gtccaggatc acctccagca ggtcgtctgg ctgattgatc tgggctagct
     1741 tgtcggtgat gtgggaaggc aggacgctgc gcagggcgtc caggtcatcc gtgatccgct
     1801 tacccgtcat cgtggagcgc cggccggttc ctcgattctg gcaatcgggg cagtgggctc
     1861 cgggtaagtc ccttcgaccg ttcgcagccg gcccttaact ggtttgcgca cgcgagctgt
     1921 cagccgcttt acgagcacgg cctggtcgac ctccggctcg aaccacaaac gctcgagcgg
     1981 ccgacccatc cagctctggt aggaccgcac gcacacgaac aacggccggt cgaccggcgt
     2041 ctgatggagg aaattcgcca tcagccggta gaagtcctcc gcggcgggat ggaaaaaggg
     2101 ctgcacccag gtccccaacg agccgcgctc aatgtccagg tatccgagaa gttcttcgtc
     2161 gcgccagtga accagattat ggccggagct ggaaggtcca gcctcaacct gctggatcag
     2221 ggcggggacg atattaaggt agaggctctg ggcagccagc gaatcggaag agctctccag
     2281 ttgccaggca atgccttgcg ggtccgggag atcctgtcgg tcgttgccca agcgccagat
     2341 gcgctggcgg gcatacaccg cgaatccggc ctggcgcaag cggtcgaagg caaagctgtg
     2401 ctcatcgaca tcggccacca ggttgaatgc gccgtgttct cccgccgtga acgcaagcga
     2461 ctcgaccagc tgcatgccca ggtcgtgctg cagcgcttcg atcggcccca ggaacgtgat
     2521 gcgggccacc ggcgaatcgc cgcggtggat gcactggccg accgcatcgt ggtcgccctc
     2581 ccgagctacc agggtgatgg ccgaacggcc cgggataaaa acatccagaa gggcattctg
     2641 aaaggcattg gcgccccggg tgaacgccag ctgcgaatcg gggcagatcc cgcggtcccg
     2701 cacccggtgg agcaaaggca ggtcgcgcca gtccagttca cgaatcatcg gtggtgaagg
     2761 gccgcaaatc gagcggtgcg gccgaggtcg cggattctac catgtaggcg atcatcgggc
     2821 tttctcttcc gctcgcgcaa cgaagtgaag ggtgcattgg gcacgcctcc gggtgttgag
     2881 agggcgccgc ggtacaatca gtccgactgc tgtgcggcgg agatcgcgat gaacgctcga
     2941 cgagtgtcgc taagtgcggt gagtattgcc ctggtgtttg tgctcattcg ttctattcaa
     3001 gtgccgatag tggcaaccga gggctttaca cacccaggcg ctgtggctga gatcttcgtt
     3061 tccctggcct tcggcccttc aattgggttg gtcgcagcag caggaggcgc ggctctggcg
     3121 gacctcacct cagggcagtt tgccaagttc gcaccactaa cactacttgc ccatgggaca
     3181 ttaggcttcc tggctgggta tctagggagg aaaaaacagt ggcccaacat gctagcagga
     3241 tggctcgttg gcggtattgc acttgtcgga atctacttcg tcggggaatc gctagtggca
     3301 aacctgtacg gaggatacgc ggcggctctc atagagctcc ctttcaatct cgttcaagtt
     3361 ggactaggca tcttcggcat ttttctttac cgggtaacta gggctgcata cccacgaata
     3421 gatcaactga ctgagcatcc taggtttgag gaggtctaga tcggcaagcg ttctaaaacc
     3481 tccgccaatc aatcggcccc cctctgctca tccgcacatc cagcaaaccc atcaaatacg
     3541 caatatctct aaagctgtca tgaccactgc ggtcgtcatc caggacctta gatacgccta
     3601 tccaaacggc gccgagacgc acgaggcttc ttgggttctc cgcggcgtcg atatggtggt
     3661 cgatgaaggt gaatttctct caatcgtcgg cccgaccggg tcgggaaaga gcaccttatg
     3721 cctggcactc aacggggtcg ttcccaacac aaccgggggg agaatgcgag gcgatgtgtc
     3781 cgtctttgga gtgaatacga agaagcaccc ggtggcggcc ctagcaaaac tggtcggagt
     3841 ggtgtttcaa gatcctgaga ctcaactttt caacatggat gcggaggcag aggtcgcttt
     3901 cgggttagaa accatgggca tgccgtcgga ggagatcggt gagcgagtgg actgggcgtt
     3961 ggctctaatg ggccttcgcg agatgcgaga tcgttcacca ctccagctgt ccggtgggga
     4021 aaagcagcgg ctagctattg catctgtcct tgcgatgact cctaaggttt tggtcctgga
     4081 cgagccaacc aacaacctgg accctcaagg gaagcgggat ctgtttacag cgctttggga
     4141 cctacgccaa tccctcgggt tgacagtcat tatggtcgag caagagaccg agtttgtcgc
     4201 ggagttctcg gacagggtgg ccctcatgcg agatggccat ttggagatag tggggccgcc
     4261 cgcaactgtc ctcggtgacg ttgagcgaat gcgggagatt ggagttgcga tccctcaagt
     4321 aacagaggtg gcatactgcc tcaacaggga cttcggtttg ggcttggcgt tcacacggtt
     4381 cggagaggct ctactgagcc tggaagcgaa attgcatgaa agctgagaaa gacccttctc
     4441 gatttgcagc tgtctgcctg gaaatcagag atctgtggtt tacctatggc gatggccagg
     4501 acgtgttaaa tgggatttca ctcagtatcg cggaagggga ctttgtggcc gtgatcggac
     4561 agaatggctc gggtaagaca accctagcta agcacttctt ggggttacta cggccaacca
     4621 agggcgagat actccttcat ggcgaaagga ttcgcgatag gtctgttgga gagttggcgc
     4681 gccaagtggg ctacgtcttt cagaaccctg atcaccagat ctttggcacg acgacttggg
     4741 aagaaatcgc cttcggaccg cgcaatcttg gcctttcggc cgaggaagtc gagcgacgaa
     4801 cagtagatgc gctggagcga ttcgatcttt catctgaggc caccaagccg cctgcaatgc
     4861 tgagttttgg tcaaaggcga aaggtaagtt tggcagctgt ttatgcgatg gaccctcatg
     4921 tattgattct ggatgaaccc actaatggcc tagatgggag gggcgagaag gaggccatgg
     4981 agctggcccg gacgctgaat aacacgggaa agactatcat tctagttacc caccgcattc
     5041 gtctcgttgc cgagtatgct cgcagctgca tccttctcgc aggcggccgt gtttcagcct
     5101 atggggatac caggtcggta ttcgagcagg acgatctact gaagtcgagc aacattgaac
     5161 tgccccaagt gtgggagttg ggtcgccagc tggcaccaag aggcttccta cagccggctc
     5221 taagcgttgg tgacttctgt caaacctacg gggagctatt gaagcaaacg gggtttagga
     5281 atgccggtgc tcgctgactt ttttatgagg ggggatagtt ggttgcataa gacggatcca
     5341 cgggtaaaag tactgtccgt gggtctgact ttggccgccg ctctgatact tcaaaacctc
     5401 ctgatgatgg tgatactgtg gttgctcgtg cttgcggtgc ctttcgcggc gagagctcca
     5461 cgaggccgaa tccgtttcgt actttctgct cttgttccgg tgagcgttct gatgcctctg
     5521 gtatggatgg tcttctatcc tattggcgtg gaaactatca gcttctggtt tatagaagtc
     5581 aaactactaa gtctggttca aggtctcaca gtcgtgctcc ggatccatat agtcagtttc
     5641 tcggtcttcg cgttactgtt ctccacggat caaaccacgc tggtgcgtgg cttggtaaag
     5701 ctacgactcc cttacgtatg gggcctcgtt ctctcattgg cacttcgcta catccccaca
     5761 ttcgagcggt cttttgcgga cattctacaa gcacagaaag ccagaggact cgagtttgag
     5821 tcgatgcgcg gttggcgccg cgtccgcgcg ttgatgcccg tcttcgttcc gacagtgatt
     5881 agtttgttcc gtctgagtga gcagataggc atcgccctgg aggctagagg tttcggggca
     5941 gagggcgttc gccggactta ttttagagac atcacgttcc gcaccctgga ctggctctac
     6001 atcgcgattg tcgtgactgc ggtggggagc ttgttctatt tcaatctcaa atatggattc
     6061 ggcgcggacc cgctgcgctt ggtacgttag gggctcgtgg tccgaccctc cgagccgttc
     6121 ccgctgggca agatccccaa cccggtctta cgcgagctgt tggccggcct cggctcgacc
     6181 gacccgcggg ttcgggtggg accaggcatt ggactggacg ctgcggtgat tgacctgggc
     6241 gaccgctacc ttgtggcgaa gaccgatccg attaccttcg cctctgattg ggttggctgg
     6301 tatgccgtgc acgttaacgc caacgacgta gcctgtatgg gtgcagaacc gcgctggttc
     6361 ctggccagcc tgctgctccc ggaaggcaag accgatcggg ggcttgcgga gacgatcttc
     6421 agtcagatcg gggaggcttg ccactcgctg gagatctcgc tggtcggtgg gcacaccgag
     6481 atcagccacg gaatcgatcg gccgatcgtc gtcggctgcc tgctggggga agtggcaaag
     6541 gacggcctgg tcactgcggc cggtgcgcaa gctggggatg tgctgctgct ggtcggaggg
     6601 atcccaatcg aggccaccgc gatcctcgcc cgggaaaagg ctggcgagtt ggaggggcgc
     6661 ttcgcgccac aattcctgga gcgctgtcgg aagttcctat tcgagccggg tatcagcgtc
     6721 gtgcgggctg ctcgcctggc ggtaaccgcg ggccagatcc atgcgatgca cgatctgacc
     6781 gagggtgggc tattggcggg cctatgggag ctggcggaag cttccgggtg cgggctggaa
     6841 gtcgatcggg aagcgatcct gatcctgcca gaggggcagg cgttgtgcga ggcctttggg
     6901 ctggacccgc tcgcctcgat tgcctcgggg gcgttcctgc taaccgctcc ccacgaccgg
     6961 cagccggcaa tcgagcaggc gctgtctgca cagggcatcg tggtcagcgc gatcgggcag
     7021 gtgatttcgc gggccggatc ggaagtgagg ctgcgatcgg cgggcgggat cgggctcgct
     7081 ccgaagccgg aacgagacga aatcgctcgc ctcctgcaac gatcggtctg atgtggtaca
     7141 atagcacaat tatacattag tacaatcaaa caatcagaca atcgggatgt gcgagtgggc
     7201 ctcgatctcg ccgggggtgt gggcctgcaa tcccccgtgg cacgcggagg caggtggttg
     7261 agacggcgga ttgcggagga gggtcggggt gagggatccg cgtggaagag cttcgtgagt
     7321 cagtagggat ggcttgagcg atggtcgcgc tggacttccg cagcaaggaa cccatttacc
     7381 ggcagatcat gggccagatc caggaacagc tcgcctccgg cgaactgaag ccggggcagc
     7441 agcttcccac cgtgcgcaag ctggcgaccc agctcggggt gaacttcaat accgtcgccc
     7501 gagcctatcg actgctggac cagcaggggg tgatctcgac ccagcatggc cgcggtacgt
     7561 atgcgctggg tcgccggtcg gtggcccgcc cgcgccggac ccggctggaa cgacttgccc
     7621 aaggattcgc ccgggaggcg gcccgactgg gttttcgtcc ggagcaggtg gccgagacga
     7681 ttggctatgt tctggaagag tgggaccagc gcggtcgacc ggtggggtag gcacgtttca
     7741 cgtggaaccc ctggcgtagg agcaaagcga tcgacattct ggcgcgtttg ttgaacgggc
     7801 ttttgatgct ggccatgccg ctggggctgg gggtcgtcct aagccggcgg accggcagac
     7861 gctggggcct gtacggggcc ggggcgctga ccttcatcgg ctctcaagtc ctgcacttgc
     7921 ccttcaacgg cctggtcctc aacccccaat tggcggctct tggtttcggc gaacgcggct
     7981 tgagcgggac gctcgtgctg tcggcggtcc tgctcgggct gtcttcgggc gtattcgagg
     8041 aaggggcgcg gttcctggtg ctgcgcttct ggcagcgctc ggcccgcagc tggcgggatt
     8101 cgctcatgtt cggcgccggg cacgggggga ccgaggcgat cctggtcgga gccctggcat
     8161 tggtcgcttt cttccaggcg ctggcatacc gcaacaccga ccttagtcag atcctgccgg
     8221 cggatcaagt cgccacggca gcctcccaag tggaggccta ttggacggct ccctggccgt
     8281 tcgcgctcct cggggcggcc gaacgagcat tctcgctggc tgcgcacctg tcgctgtcgg
     8341 cgatggtgat gcgggccttt acccaccgaa atgccggctg gctggtagcg gcgatcggct
     8401 ggcacgcgct gatcaacacc ctggcggtga tcgcgctgtt tacggtcagc ccagttgcga
     8461 ctgaaggctt gatcgggctg accgccgggg tgagcgttgg gatcctgttc gcgctccgcg
     8521 aaccgccggt cgtggtttca gcagagccgg gcgagcagct ggcccgtgtc acggcggcga
     8581 aagccagctt gccgctggct cgggtggagg actcaaagta tgccgagtga caggtgggta
     8641 cttacgattc gagagcgaga gggaatcgat gattaggatt gaggcgctct ccaaacgctt
     8701 cggctcgacc gtagccgtgg agaatctgtg gctcgagatc gcccccggtg agctcttcgg
     8761 ctttttgggg ccgaacggcg ccggaaagac cacgacgatc cgaatgctga ccggactgat
     8821 aagcccgagc agcggccggg cctgggtggc gggccatgca ttgggcgagg acgacactga
     8881 gcttcggcgc caggtcggga tcctgaccga ggcgcccggc ctgtacgaca cgctgagcgc
     8941 cgagcgcaac ctggccttct tt
//
LOCUS       DATDPQ010000324         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1498255, whole genome shotgun sequence.
ACCESSION   DATDPQ010000324 DATDPQ010000000
VERSION     DATDPQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498255"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VI520_07305"
     CDS             complement(<1..592)
                     /locus_tag="VI520_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF37724.1"
                     /translation="MDQPVKRWIGLSEAARLLGVHPSTVRAWADGGKLQVHRTPGGHR
                     RFSLPEIERWGADRGRRASEAQVVIENVIGRTRLEAPRLQNQPWYLKLTETQRADYRA
                     ESHRLLGQVGRFIGLAAEGAEPEAAQIGQDYARISLQAEMTLIEAVEAFLFFRSFLME
                     SLFSLSEFQSAPSWDELHRETSHFADLVLLALIEAYQ"
     gene            652..1677
                     /locus_tag="VI520_07310"
     CDS             652..1677
                     /locus_tag="VI520_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37725.1"
                     /translation="MDWGLIGNEQAVTGLKRHIVEHQVRHAYLFTGPNAIGKRTLGLR
                     FTQALNCERPGRPGDFCSPGDPAACRVCRAVPQGMDPDLHILTPQESIRVDEIRLLQS
                     RLALAPYGGRWRVALLPDFHQATASSANAMLKTLEEPPSRVVLLLTSPEQELLLPTVV
                     SRCEVVALRGVATTTIARALLERGATPERAELLAAVADGRPGRAIALAEDSAAAEERT
                     AALRDFSDLMQMDLNQRFATVERLIGKGELPDQRRRVESTLLEWMSLWRDLVHQGYGA
                     ELVSRNPDFAPAVGARASQLTAGERQSTFRSLAASLQAVEANANLRLTLEVLLLDLPR
                     VGAGPIE"
     gene            complement(1700..>2366)
                     /locus_tag="VI520_07315"
     CDS             complement(1700..>2366)
                     /locus_tag="VI520_07315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HLF37726.1"
                     /translation="RLMKWFVAVAGNIGVGKSTLVQLLSARLDWTPFLEADVQNPYLA
                     DFYQDMPTWSFQSQIFFLSRRLRAHREIADHPGSGIQDRSVYEDAEIFARNLHLEGHL
                     TERDYRTYRELYEVLTKFLPPPDLVVFLRASVDTLLQRIAQRARAYERGIGAEYLSQL
                     NQLYEDWAEQFTLCPILSVPADNLNYVLHDAHLDLIVSKIRDKLSGKEEVRFEAEEVA
                     KLN"
BASE COUNT          440 a          706 c          787 g          433 t
ORIGIN      
        1 gctgataggc ctcgatcaag gccagcagga ccaagtcggc gaagtgcgaa gtctcccggt
       61 gcaactcatc ccaggaaggt gcagactgga actcagagag actgaacagg ctttccatta
      121 agaagctgcg gaagaagaga aaggcctcga ccgcttcgat cagagtcatc tcggcctgca
      181 gactgatccg ggcatagtcc tgaccgatct gggcggcctc cggttcggct ccttcggcgg
      241 ccaagccgat gaaacgcccg acctgcccaa ggagccggtg ggactccgcc cgataatccg
      301 cccgctgagt ctcggtcagc ttgagatacc agggttgatt ctgtaaacgg ggggcttcta
      361 gccgggtgcg tccgatcacg ttctcgatga cgacctgggc ttcgctcgcc cgccggccgc
      421 ggtcggctcc ccaacgttcg atctctggca acgagaagcg tcggtggccg cctggagtgc
      481 ggtggacctg aagtttccct ccatcggccc aggctctgac cgtggaaggg tgcaccccca
      541 gcagccgggc agcctcggag aggccgatcc aacgcttgac gggctggtcc attcgaactc
      601 aatctatatc aatcgaggta agttcgtcaa ccgctgggat agaatggcca cgtggattgg
      661 ggcctgatcg ggaatgaaca ggcggtcact gggctgaagc gccatatcgt cgagcatcag
      721 gtccggcacg cctatctgtt taccggcccg aacgcgatcg gcaaacgcac tctcggcctg
      781 cgctttaccc aggcgctcaa ctgcgaacgg cccggccgtc cgggggattt ctgctctccg
      841 ggcgacccgg ccgcttgccg ggtgtgtcga gcggttcccc aggggatgga tccggatctg
      901 catattttga ctccccaaga gtcgatccgg gtcgatgaga tccgcctgct gcaaagcagg
      961 ctggcgttgg ctccctacgg ggggcggtgg cgagttgccc tcttgccgga cttccaccag
     1021 gcgaccgcaa gttcggccaa cgcgatgctg aagacgcttg aagagccgcc ctcccgagtc
     1081 gtgctgctgc tcaccagccc agagcaggag ctgctgctcc ccaccgtggt ttcgcgttgc
     1141 gaagtcgtgg cgttgcgggg ggtggcgacg accaccatcg cccgggcgtt gttggagcgg
     1201 ggagccacgc cggagcgggc cgagctgctg gcggccgtcg cggatggccg acccgggcga
     1261 gccatcgcct tggccgagga ttcggcggcc gccgaagagc ggacggccgc cttgcgggac
     1321 ttctcggatt tgatgcagat ggatctcaat cagcggttcg caaccgtgga gcggctgatc
     1381 gggaaagggg agctgcccga ccagcggcgt cgagtcgaga gtaccttgct ggaatggatg
     1441 agcttgtggc gggatctggt ccatcagggg tacggggcgg agctggtttc ccgtaaccct
     1501 gacttcgcgc cagcagtggg agcccgggca agccagctga cggccggcga gcgccagtcc
     1561 accttccgtt cgctggcggc ctcgctgcag gccgtcgagg cgaacgccaa tctccggctg
     1621 acgctggaag tgctgttgct tgatttgccg agggtgggag cggggccgat cgaatagggt
     1681 aagcaggccg ccgccgaggc tagttgagct tggcgacctc ctccgcctcg aaccgaacct
     1741 cctctttccc ggataacttg tcgcgaatct tgctcacgat caagtccagg tgcgcatcgt
     1801 gcagaacgta gttcaaatta tcggccggca cgctgaggat cggacagagg gtaaactgct
     1861 cggcccagtc ttcatagagc tggttcagtt gtgagaggta ctcagccccg atccctcgct
     1921 cataggcgcg ggcacgctgg gcgatccgct gcaaaagggt gtcaaccgag gcgcgcagga
     1981 aaactaccag gtcgggcggg ggcaagaact tcgtgagcac ttcgtacagc tcacggtagg
     2041 tccggtaatc gcgctcggtg aggtgacctt ccaaatgcag gttgcgggcg aagatctcgg
     2101 cgtcctcata aacgctgcga tcttggattc cggagccggg gtggtcggcg atttcccgat
     2161 gggcccgcag tcggcgggag aggaagaata tttgcgactg aaacgaccaa gtcggcatgt
     2221 cctgatagaa atcggccagg tagggattct gcacgtcggc ttcgaggaag ggagtccaat
     2281 ccagccgagc tgagagcaat tgaaccaggg tcgatttgcc gaccccgatg tttccggcca
     2341 ccgccacaaa ccacttcatg aggcga
//
LOCUS       DATDPQ010000325         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2025047, whole genome shotgun sequence.
ACCESSION   DATDPQ010000325 DATDPQ010000000
VERSION     DATDPQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025047"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VI520_07320"
     CDS             complement(<1..1216)
                     /locus_tag="VI520_07320"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HLF37727.1"
                     /translation="MNFQEALDFLYSFIDYGRERTYRYSPEVFDLGRVRRLLGSLGDP
                     QEGFPSVHIAGTKGKGSVAAMIEASYRAAGYRTGLYTSPHLIRFTERIRVDGREIPEP
                     DLVRLVERLKAVAPEIPQISTFELTTALAFLHFAELKVNCGVIEVGLGGRLDATNVLT
                     PTVCVITSLSYDHVHLLGASLSDIAREKAGIIKPTVPLVLAPQQSEAEQVIRSIAEQR
                     GAPLYQVNRDWHYAAEQRSLTGQSFRVWKDTHGAPVRLSIPLLGHHQIQNACTAYAAL
                     QLASERGLPVGLEAIRAGMGSVRWAGRFQVLSVDPSVVLDSAHNRDSALQLRIALDDY
                     FPGRPVRMVFGASSDKDVRGMLLELSPRVSRVILTQADHPRAEGPEALLGLIRSFGLP
                     VETVVPVADALRI"
     gene            1355..2209
                     /locus_tag="VI520_07325"
     CDS             1355..2209
                     /locus_tag="VI520_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLF37728.1"
                     /translation="MTRHAARAAPDAQVRFTTRLADLPWWALAIALGGVVLIYNFATN
                     AVYGEVLSNIVSGVKLTILVSVSAYAIAIVLGLTTSLGQLSKNTVARNGALLYVQVVR
                     GIPILVQIFTVAFVVVPLAIQAINALGHTLIGAGILPQDNWLSSLNVRNVDFVARGII
                     ALALSYGAFSSEIFRAGIQSVERGQIEAAQSIGLSYFQSLRLVILPQAIRRVLPPLGN
                     DFIAMLKESSLVSAIGVGEITQLSKKYAAASFKFPETYFTLAFVYLSMTLVLSMLVRF
                     MERRLKSG"
     gene            2346..>3560
                     /locus_tag="VI520_07330"
     CDS             2346..>3560
                     /locus_tag="VI520_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLF37729.1"
                     /translation="MKKLVFLLSLLLLGAFVVAACGQAEPATVEAPPEVVEGALPDLG
                     GREITIAIENAYLPFNFVLLETGEAQGWDYDTVGEICRRLNCVPVWQEFAWDTMIASV
                     SESQFDMAADGITITEERAQQVDFSIGYIAIEQRLLVRIDEARFEGPDDFASNPELIL
                     STQIGTTNADTAVALVGDDRVTLFDDFGLVVQALIAGDVDGVIIDETAGLGYLGANKE
                     QLKLIGRSLSSDELGFVFPNGSDLVEPFNAALRSMMADGTLEEINAKWFGPDFVLTYD
                     EVGPGAYAEEAVAIGTADSPIKVLFVPSVNVDYMVSSGDAIEQFLSDATGLVFEVSVP
                     TSYAATIEEMCASTTDTVGFIPAMGYALANQLCGVQPALASVRFGWEFYWAQYIVARD
                     SAFQTLEDLEGAK"
BASE COUNT          676 a         1106 c         1062 g          716 t
ORIGIN      
        1 ctatgcgcaa ggcatctgcc accgggacca cagtctcgac cggcaggccg aagctgcgga
       61 tcagtcccag cagtgcctca ggcccctccg cccgcggatg atcggcctgg gtaaggatca
      121 ctcgcgacac ccgcggcgac aattcgagca gcatcccccg cacatcctta tcgctcgaag
      181 ccccaaacac cattcggacc ggccgtcccg gaaagtaatc gtccagcgcg atccgcagtt
      241 gaagggccga atctcgattg tgagcggagt cgagcactac gctcggatcg accgacagca
      301 cctgaaacct gcccgcccag cgcacactcc ccatcccggc tcggatcgcc tcgagtccga
      361 ccggcagccc acgctcggag gccagctgca aggcggcgta ggccgtgcag gcattctgga
      421 tttgatgatg ccccaataac ggaatgctca gccgcaccgg cgccccatga gtgtccttcc
      481 atacccgaaa gctctgcccg gttaagctgc gctgttcggc cgcgtagtgc caatcacgat
      541 tgacctgata caaaggggcg ccgcgctgct cggcgatcga tcggatcacc tgctcggcct
      601 cagactgttg gggggccagg accagcggca cggtcggctt gataatcccg gccttctcgc
      661 gggcgatgtc ggacaggctg gcacccagaa ggtgcacgtg atcatacgaa agtgaggtaa
      721 tgacgcagac ggtgggggtc agcacgttgg tcgcgtccag ccggccacca agaccgactt
      781 cgattacccc gcagttcacc ttcaactcag cgaaatgcag gaaggccagc gcagtggtca
      841 gttcgaaggt cgagatctgg gggatctccg gggcgactgc cttgaggcgc tctaccagcc
      901 ggaccaggtc gggctcgggg atttcccggc cgtcgacccg gatccgctcg gtaaaccgga
      961 taaggtgggg cgaggtgtac agcccggttc ggtagccggc ggcccggtaa ctggcctcga
     1021 tcatcgccgc caccgaccct tttcctttgg tccctgcgat atggacgctc ggaaacccct
     1081 cctgcgggtc gcccaacgag ccgagcaacc gccggacccg gccgagatca aagacctcgg
     1141 gcgagtagcg gtaggttcgc tcccggccgt aatcgatgaa gctgtagagg aaatcgaggg
     1201 cttcttgaaa gttcacagcc gcgattgtaa attgcagaga tcaaaggttc aagccggccg
     1261 tggccaagcc ccgtttggca cccctgagga cctcgggtat gattctggaa tcctccctag
     1321 atccaacttg aactcgactg gctcttcact agacttgacc cgtcatgctg cccgggcggc
     1381 tccggatgcc caggttcgat tcaccactcg cctggcggac ttaccctggt gggccctggc
     1441 gatcgcgctt ggcggggtgg tgctgatcta caacttcgcg accaacgcgg tctacggaga
     1501 ggtgctgtcg aatattgttt ccggcgtcaa gttgaccatt ctggtgtccg tgagtgcgta
     1561 tgcgattgcc atcgtgctcg gcctgacgac ctcccttggg cagctctcca agaacaccgt
     1621 cgcccgtaat ggcgcccttc tttacgtcca ggtggtccgt gggatcccga ttctcgtaca
     1681 aatcttcacg gttgcctttg tcgtcgtgcc gctggccatc caagcgatca atgcactggg
     1741 ccataccctc atcggcgcag gtattctgcc ccaggacaac tggctctcca gcttgaacgt
     1801 tcgtaacgtt gattttgttg cgcgaggcat aattgcattg gccctcagct atggcgcgtt
     1861 ctcctcggaa atcttccgcg ccgggatcca atccgtcgag cgaggccaga tcgaagcggc
     1921 tcaatcgatc ggcctcagct acttccagtc gctgcgcctt gtcatcctgc cacaggcgat
     1981 ccgcagggtc ttgcccccgc ttggcaacga ctttattgcg atgcttaagg agtcttccct
     2041 cgtatcggcc attggggtcg gtgagatcac ccagctcagc aagaagtacg ccgcggcctc
     2101 cttcaaattc cccgagacct acttcacctt ggcctttgtg tacctctcca tgacccttgt
     2161 gttgtcgatg ctggtacggt tcatggaaag gcgtctcaag tcggggtaga attcaagctg
     2221 catttgaagc tcacaaccct acctcaccaa cgccaaaagg aggtggtgcc tgagttcgcc
     2281 cagactcggt tgacacatcg ttcatcacat cccgcaagac acaaagaatc caggaggaga
     2341 aatctgtgaa gaagcttgtg tttctcttaa gtttgctgtt gcttggcgcc ttcgtggtgg
     2401 ccgcctgtgg gcaagccgaa ccggcgacgg ttgaggctcc cccggaggtg gtggaaggtg
     2461 cgcttcccga cctcggcggt cgagaaatca cgatcgccat cgaaaacgcc tatctgccgt
     2521 tcaacttcgt tctgctggag accggcgagg cccagggctg ggactacgac acggtcggtg
     2581 agatctgtcg gcggctaaac tgcgtaccag tttggcagga attcgcctgg gacacgatga
     2641 ttgcctcggt ctccgaatcg cagttcgaca tggcggccga cgggatcacg atcacggaag
     2701 agcgggccca gcaggtggac ttctcgatcg gctatattgc aatcgagcag cggctgctgg
     2761 tacgcatcga tgaggcccgc ttcgaagggc cggacgattt cgccagcaac ccggagctga
     2821 tcctctccac ccagatcgga accaccaacg ccgacactgc cgtggcgctg gttggcgatg
     2881 accgcgtcac cctcttcgat gactttggcc tcgtcgtcca ggcgctgatc gctggggatg
     2941 ttgacggtgt gatcatcgat gagaccgccg ggctcggata cctcggcgcc aacaaagagc
     3001 agctgaagct gatcggccgc tcgctatcca gcgacgagct cggctttgtc ttcccgaatg
     3061 ggagtgactt ggtcgagccg ttcaatgcgg ccctgcgctc gatgatggcg gatggcacct
     3121 tggaggagat caacgcgaag tggttcgggc cggatttcgt gcttacgtac gatgaggtcg
     3181 ggccgggcgc ctacgccgaa gaggcagtgg cgatcggaac cgccgacagc ccgatcaagg
     3241 tgctgttcgt gccctccgtc aatgtcgact acatggtctc cagcggggac gctatcgagc
     3301 agttcctcag cgatgccacc gggctggtct tcgaagtcag cgtcccgact tcttatgccg
     3361 ccaccatcga agagatgtgc gcctcgacga ccgacacagt cggtttcatc cccgcgatgg
     3421 gctacgccct tgccaaccag ctgtgtggtg tgcagccggc gctggcttcg gtgcgcttcg
     3481 gatgggagtt ctactgggct caatacatcg tggcacgcga tagcgcgttc caaactctag
     3541 aagacctgga aggcgcgaag
//
LOCUS       DATDPQ010000326         4280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2801215, whole genome shotgun sequence.
ACCESSION   DATDPQ010000326 DATDPQ010000000
VERSION     DATDPQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801215"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VI520_07335"
     CDS             <1..322
                     /locus_tag="VI520_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37730.1"
                     /translation="KVGPLDPTFYYAMDYDLWVRIARIAPIAFHERYWSNFRLHSQSK
                     SMTAAARSWPEIMRVHFRDGGSVFSVIYAKYLLRRLLEPVMPWRVRLRLFRYGLEQKV
                     RWGD"
     gene            complement(319..1551)
                     /locus_tag="VI520_07340"
     CDS             complement(319..1551)
                     /locus_tag="VI520_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF37731.1"
                     /translation="MRLTLINQFYPPDFAPTGELAASLAEHRVQLGDQVTVVTSQGGY
                     VPESPVEAAPPTSNPRIHRLWTPRLGKQTLALRLIDYSLFYAQAAIRLALLPQQDLIV
                     SLTTPPLIALAGALHKWLHRETRLILWSMDCYPEVPERAGVLRPGSIPSRFIRWLNRR
                     LFRALDYLVCLDTPMEQLLKAQYESSNPKLPTSVIHNWEPAMSYPKDLLPPPWPMAGE
                     LDLSGKFVVLYLGNAGYGHRFETVLDAADQLRTEPVIFVFIGGGQKWSELKRTKEQRG
                     LANLHVLRYVPDAETQSAMAAADCALVTLRDSYLGVISPSKIHSNLAMGLPLIYIGPE
                     GGNVDEAIRLYQCGVSLHHGDVDGTVEFIRRLQAQPKQLKVFRSQARRAFEEAYSDVV
                     CLPQFDRVLEQVMGRSPA"
     gene            complement(1564..3375)
                     /locus_tag="VI520_07345"
     CDS             complement(1564..3375)
                     /locus_tag="VI520_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37732.1"
                     /translation="MRPKLRFLLCHLALWLPLGAAVAAVLAASFGSLLGLDPDLKWGG
                     VRLMVLGGGLAVLGIFAADRILARIDLGLLRRNKPQLSEGTPPVLTSEPITGARSMTL
                     PRGQLALFFLTTIFIYLGLATVWNWPRWPKSTYRYDSLAEAFAKGNVALSIQPSPALA
                     DLDNPYDPAAREGIQVISDLSYFDGRYYMYWGPTPAALSAVWLLAGGSPISDALIVFL
                     SISVMFVFSALLVLDLQRKYFPNMPPWLVAGSLVLVATAHPALWTLNSPSIYTAAISS
                     GQAFFVGGLYFMLTTLNAPPTAPWRHVACGTFWALAIGSRLTLLVTVGPLVLVVIALR
                     LRAMLGSRSGKQELYQIGALLMPLALGGILLGAYNFARFGDALESGFRYQIVENDQGE
                     AMRQGLVFNPRFLIPNLLHYLATPIRLGPEFPFVRPRWEAYPPFASFLARFKIPQEHS
                     VQDAAGLIFVTPALLLGGPLLMSALSCPSGAGQNPLNGRGVMSASLTLRHFVLVLLLS
                     GLTLTLPILAYRVSSIRFLMELSPVMAVFASLGAFFLYQNTRSLPSRRLAVISLITSA
                     IALSALIGFLLALNGADSRFDDANPTLYQALVDLFSR"
     gene            3693..>4280
                     /locus_tag="VI520_07350"
     CDS             3693..>4280
                     /locus_tag="VI520_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37733.1"
                     /translation="MPAAKSNPLSSQKGPTPALLYSALLSHWPRVFVLGVLGGLLGLG
                     ASWVSPARYLATARISVGIDYGRAQFADEDAERHILARVQDLLLSDEALERALDSFVV
                     AGEAAPSPGGFREQIRLTRIEAEWSLGVTDPSPGRAAGLASAWAAASLGLLEESQQEA
                     WKLAELQALFFQFACRPPAPGESGDPPVWLCDELQG"
BASE COUNT          914 a         1234 c         1370 g          762 t
ORIGIN      
        1 caaggtgggg ccgctcgatc cgacctttta ttacgcgatg gattatgacc tgtgggtgcg
       61 gatcgccagg atcgcgccga tcgcattcca tgaaaggtat tggagcaatt tccggctgca
      121 cagccaatcc aagtcgatga ctgccgccgc ccgcagctgg ccggaaatca tgcgggttca
      181 cttccgagac gggggaagcg tattctccgt tatctatgcc aagtacctgc tgcggcggct
      241 gctcgagccg gtgatgcctt ggagggtgag actgcgcttg tttcgctatg gcctggagca
      301 gaaggtccgc tggggcgact aggcagggga acggcccatg acctgctcca acacccggtc
      361 gaattgcggt aggcacacaa cgtctgaata ggcctcctcg aaggcgcggc gggcctggct
      421 cctgaagacc ttcaactgct ttggttgcgc ctgcagcctc cggatgaact caaccgttcc
      481 gtccacgtcc ccgtggtgta ggctgacccc gcactgatag agccggatcg cctcatccac
      541 gttacccccc tccggcccga tgtaaatcaa gggcagcccc atggccaggt tggagtgaat
      601 cttgctcggg ctgatcacgc caaggtacga atcgcgcagc gtcaccaatg cgcagtcggc
      661 cgccgccatc gcggattggg tttcggcgtc cggaacatag cgcagcacat gcaggttggc
      721 cagcccgcgc tgttctttgg tccgcttcag ttcgctccac ttctgaccgc cgccaatgaa
      781 cacgaagatc accggttcgg ttcgaagttg gtcggcggca tccagcaccg tttcaaaccg
      841 gtggccatag ccagcgttcc cgaggtagag caccacgaac ttcccgctga ggtcgagttc
      901 cccggccatc ggccatggag gcggtagcag gtctttggga tagctcattg cgggttccca
      961 attgtgaatt accgaggttg gcagcttggg gttggaactc tcgtactgag ccttcagcag
     1021 ctgctccata ggggtatcca ggcataccag atagtccagg gcccgaaaga gccgccggtt
     1081 gagccagcgg atgaaccggc tcggaatact tcccggacgg agcacgccgg cccgctccgg
     1141 cacctctggg tagcagtcca tgctccacaa aattagccga gtctccctat gcagccactt
     1201 gtgcagcgcg ccggccaatg cgatgagggg cggggtcgtc agcgaaacga tcaggtcctg
     1261 ctgcggcagc agagccagcc ggattgcggc ttgggcgtag aacaagctgt agtcgatcag
     1321 ccgcagggcg agggtctgct ttcccagccg gggggtccac agccgatgga tccgcgggtt
     1381 tgaagtggga ggggcggcct cgaccggcga ctcgggaaca tagcctccct gactggtgac
     1441 caccgtcacc tgatcgccga gttggacccg gtgttcggcc aacgaggcgg ccagctcacc
     1501 ggtgggcgcg aaatcggggg gataaaactg attgatcagg gtcaggcgca tacctgccgg
     1561 atttcagcgc gagaagagat ccaccagagc ctgataaagg gtagggttgg catcatcaaa
     1621 cctcgagtca gccccattta gagcaaggag aaacccgatg agtgcagaga gggcgatcgc
     1681 cgaggtgatg agagagatga ctgcaagccg acgggacggt aggctacggg tgttctggta
     1741 gaggaagaac gcacccagcg aagcaaagac tgccattact ggggagagct ccatcaggaa
     1801 ccttatggac gaaacccgat aagcgagaat cggtaaggtc agagtcagtc ccgacaataa
     1861 gagcaccaag acaaagtgtc ggagagtaag tgaggcactc atcacaccgc ggccgttgag
     1921 tggattctga cccgcgcccg atgggcacga gagggcgctc ataaggagcg gtcctccgag
     1981 cagtagcgcg ggtgtgacga agatcaatcc ggccgcatct tgcacgctgt gctcttgagg
     2041 aatcttgaac cttgccagga aggaggcaaa gggcgggtag gcctcccacc ggggcctcac
     2101 gaaggggaat tcgggaccca gccggatcgg tgtggcaagg tagtgaagaa gattgggaat
     2161 caaaaagcgt ggattaaaga ccaggccctg gcgcattgcc tccccctggt cattctcgac
     2221 gatctgatag cgaaatccag attcgagtgc atcgccaaat cgggcgaaat tgtacgcgcc
     2281 gagaaggatt cccccaaggg cgagcggcat gagcagggcg ccgatctggt agagttcctg
     2341 tttccccgaa cgacttccga gcattgccct cagcctcagg gcgatgacca ccaggacgag
     2401 cggtccgacc gtgaccagca gagtcaagcg gctcccgatt gccaaggccc agaacgtacc
     2461 acaagccaca tggcgccatg gggcagtcgg cggtgcgttc aacgtagtca gcatgaagta
     2521 aaggccgcca acaaagaagg cttgtccgga agagatggcg gcggtgtata tgcttgggct
     2581 gttcagcgtc cagagcgcag ggtgagccgt cgccacaaga acaaggcttc cggcgacgag
     2641 ccatggaggc atgttgggga agtactttcg ctgaagatcg aggaccaata acgccgagaa
     2701 aacgaacatc acgctaatgg acagaaacac aatcaacgcg tcgctgatcg gggatccgcc
     2761 ggccagcagc cagacagcgc tcagggcggc cggcgtcggc ccccaataca tgtagtaacg
     2821 accatcgaag taagaaagat ccgagatgac ttggattcct tcgcgggccg cgggatcgta
     2881 ggggttgtcg agatcggcaa gggccgggga aggctgtatc gagagcgcca cgttcccttt
     2941 ggcgaaggct tctgcaagcg agtcgtaacg gtaggttgat ttcggccatc ggggccaatt
     3001 ccaaacggtg gccagtccga gatagatgaa aatcgtcgta aggaagaaca gagcaagctg
     3061 tccgcgggga agggtcatcg accgcgcgcc ggtgatcggc tccgaagtca acaccggagg
     3121 agttccttcg ctgagctgag gcttgttgcg cctaagcaag cccaggtcaa tccgggcgag
     3181 gatgcggtct gccgcgaaaa tcccaaggac agcaagcccg cctccgagaa ccatcaacct
     3241 gactccgccc cactttaggt cggggtctag gcccaacaag ctgccaaagc tggcggcaag
     3301 cactgccgcc acggccgcac ccagcggcaa ccaaagtgcc agatgacaaa gcaaaaaccg
     3361 cagcttgggc ctcaaattac tggtcattta gatggctgcc cgctgttcgc gcgccggtgc
     3421 cgcggaaagc tcgtcaagga gctaattgcc cgtgccaatg gcggaggctt cgggatcgtc
     3481 ccccgaagaa gcggacagtt tcatgccacc agtctctcat actctttggg agcgaggtag
     3541 tcaatgccgg ggtgaaggcg gtagctgcgc tcgatgcgct gcagtaggac ccctcttatc
     3601 ggatacctgc gtatcattag ggagtagtgt ccactccatt gggggaagga ttccctcggc
     3661 cggagccgca gtgttagact agcgaatcgc ccgtgccagc agccaagtcg aatccactgt
     3721 ccagccagaa gggcccaacc ccggctctgc tctactcggc acttcttagc cactggccgc
     3781 gggtcttcgt cctgggagtc ctgggagggc tgttgggact gggcgcgagt tgggtcagcc
     3841 cggctcgcta ccttgccacg gcaagaatct cggtcgggat cgattacggc cgagctcagt
     3901 tcgcggatga ggacgccgag cgccacattc tggcgagagt tcaggacttg ctgctgtcgg
     3961 atgaggccct ggagcgggca ttggactcat tcgttgtcgc cggtgaggca gcgccaagcc
     4021 cgggcggctt ccgagagcaa attcggctga cccggatcga ggccgaatgg tcactcgggg
     4081 taaccgatcc cagccctggc cgggccgcgg ggctggccag cgcctgggcc gcggcgtctc
     4141 tcggcttgct tgaagaatcc caacaggagg cctggaagct ggctgagctg caagccctgt
     4201 tctttcagtt cgcttgtcga cccccggcgc caggcgaatc tggagatcca cccgtgtggc
     4261 tgtgcgacga actccaggga
//
LOCUS       DATDPQ010000327         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3344065, whole genome shotgun sequence.
ACCESSION   DATDPQ010000327 DATDPQ010000000
VERSION     DATDPQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344065"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VI520_07355"
     CDS             complement(<1..564)
                     /locus_tag="VI520_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HLF37734.1"
                     /translation="MTAIKLEANRRIILGKQNRRLRREGILPAVLYGSEFGSFPIELD
                     RRTTSRLLARVSRSTLIELEVDEQPHTVLVREIQRDPLRGRLLHVDFLKVRMDVRIRA
                     EVPIELVGEAPAAKIAGGVLVTGVDAIEVEALPADLPDRVTVDLESLVNIDDSITVAD
                     LFIGENVRILTDLNELVARIIYEAEEII"
     gene            complement(596..1069)
                     /locus_tag="VI520_07360"
     CDS             complement(596..1069)
                     /locus_tag="VI520_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37735.1"
                     /translation="MTESEGRLSADVIHQEGGVVERVSGDEVHVSQSGVKSISGEQLS
                     VWQSGVWHVEAQDIELQQSAAVGVRGESVSLESSASAVSIAEYGELSNSRVGLVIGGE
                     LNLSNSPSLIVLARQVNGNVETVLDTRGALLAGVAAGVISGVFYLLTSMLRRPRD"
     gene            1208..1951
                     /locus_tag="VI520_07365"
     CDS             1208..1951
                     /locus_tag="VI520_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37736.1"
                     /translation="MSGPQLRSWRFLGRSAGCSLGQTRFGFLPIWLGILGFAATSAGG
                     AIRVVSQIDVPGFAEIVQPPEGQALQGIVTIRGTATHPEFEAFDLAFGFDPDPTETWF
                     ALGEPVDTPVVDGRLAIWDTGPISDGNYRLRLRVRIQGGDPLVAIVQDLRVRNYTPTE
                     APAGLVRLPATAEPEPTRLPDEALEPERPAERRPDPFRTALTVGAVAGVGLTSSLALY
                     QLFAPRARAYAGFLRMRRLHRRTGRPPRR"
     gene            1948..2535
                     /gene="pth"
                     /locus_tag="VI520_07370"
     CDS             1948..2535
                     /gene="pth"
                     /locus_tag="VI520_07370"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLF37737.1"
                     /translation="MNDSPLSLIVGLGNPGRQYRNNRHNAGFLLIDRIAREFGLRLET
                     HQSESLLATGSHAGRSFLLAKPQTYVNETGRAVAGLVKLHRVEQPNLLVAFDDLDLEL
                     GTLRMRPFGGSSGHRGMRSLINHLGDQGFPRLRIGIGRPRGRLDPAGFVLSNFASDEL
                     DLVSETLDRALGCVRRFLEAGIEKAMTDCNTRPAE"
     gene            2532..>3377
                     /locus_tag="VI520_07375"
     CDS             2532..>3377
                     /locus_tag="VI520_07375"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37738.1"
                     /translation="MIELSSLLDLIRALPQYRELRETLAARPTRGRHVPMLAAARPPM
                     AAALASEFNVPVLMICARADRVATLAQELPSWAPSTEVLTFPEPDPLFYEALPWGVRN
                     RQLRVETLARLTHPGGPQPLIILTSARAIMTRTISPEQFAAHTHELIAGSYLQLDSLL
                     KSLIGSGYEPESIVTERGQFSRRGGILDLWPTSAPAPVRIELIADEIESIRRFDPATQ
                     RSSDPVQGVQVGPAREGLPAYYQQSNDLPLPLAEAQPILEFFLPWMTPDPQTVLDFLP
                     AETLVI"
BASE COUNT          594 a         1180 c          961 g          642 t
ORIGIN      
        1 gataatctct tcggcttcgt agatgatccg ggcaaccaat tcgttgagat cggtcaggat
       61 ccggacgttt tcaccgatga acaagtccgc cacggtgatc gagtcatcga tgttgaccaa
      121 cgactccagg tcaaccgtga ctcggtccgg cagatcggcc ggtagggcct cgacctctat
      181 agcgtccacc ccggtcacga gcactccgcc ggcgatcttg gcagccggag cctctccgac
      241 caactcgatc gggacttcgg cccggatccg gacatccatc ctgactttga ggaagtcgac
      301 gtgcagcagc ctcccgcgca ggggatcccg ctggatttcc cgtaccagaa ccgtgtgggg
      361 ttgttcgtcc acttcaagtt cgatgagggt cgagcggctg acccgcgcca acagccggct
      421 cgttgtgcgc cggtcgagct caatcggaaa cgaaccgaat tccgacccgt agagaaccgc
      481 cggcagaatt ccttcccgcc gtagtcgccg gttctgcttt ccgagtatga tccgtcggtt
      541 ggcctctagc ttgatcgcgg tcatgagtct ctccgctcct gtctatattc caaattcagt
      601 cccgcggcct gcgcagcatg ctggtgagca aatagaaaac gccactgatt acgcccgccg
      661 ccacccctgc cagcagagca ccccgagtgt ccagcacggt ctcgacattc ccgttgacct
      721 ggcgggcaag cactatcagg cttgggctgt tgctcaagtt cagctcgcct ccgatcacca
      781 atcccacccg cgagttggaa agctctccgt attctgcgat cgaaaccgca ctggcactgc
      841 tctcgagcga tacgctctcg cccctcaccc caaccgcagc cgattgctgc agctcgatat
      901 cttgggcctc cacgtgccag accccgctct gccagaccga taattgctca ccgctgatcg
      961 atttcacccc gctctggctg acatgcacct catccccact aacccgttcg acaaccccac
     1021 cttcctggtg aatgacatcc gctgagagcc ggccctctga ttcggtcatc catgctcctt
     1081 cgctgtgcac aaaaaggggc gcctcacccg caaccgggtg acgccccaaa tggaaattgt
     1141 accgggccag gcttcgatcg tcaatcgccc cgcgtgttaa actaacctgt tcgcgcctga
     1201 tcgagccgtg agcggccccc aactccgaag ctggcggttt ctcggccggt ctgccggatg
     1261 ttcgctcggg cagaccaggt ttggattcct tccaatctgg ctagggatac tgggtttcgc
     1321 ggcgacctcg gctggcggtg cgatccgggt agtatcccaa atcgacgtgc caggcttcgc
     1381 cgaaatcgta cagccgccag aggggcaggc cctgcaagga atcgtcacga ttagaggcac
     1441 cgccacccat cctgagttcg aggcatttga ccttgccttt ggatttgatc cagacccaac
     1501 cgagacctgg ttcgccctgg gcgagccagt cgacacgccg gtggtggacg gccggttggc
     1561 catctgggac accgggccga tcagcgacgg aaattaccgc cttcgcctgc gagtgcggat
     1621 ccagggcggc gatccgttgg ttgcgattgt gcaggacctg cgggttcgca actatacccc
     1681 taccgaggct ccggccggct tggtccgcct gccggccaca gccgaaccgg agcccacccg
     1741 gcttcctgac gaagcgcttg agcccgaacg cccggctgag cgccggccgg atccgttcag
     1801 aaccgccctg accgtagggg cagtcgccgg ggtcggcctg acaagctcgc ttgccctcta
     1861 ccaattgttc gccccccggg ccagagcgta cgccgggttc ttacgaatgc gccgattgca
     1921 ccggagaacc ggacgacctc cgaggcgatg aacgactccc ccctcagcct catcgtcggc
     1981 ctgggaaacc ccggtcgaca ataccggaac aaccgacaca acgccgggtt cctgctgatc
     2041 gaccgaatcg cccgtgagtt cggtctgcgg ctggagactc accaatccga atcgctgctt
     2101 gccaccggat cacatgccgg aaggagcttt ctgctagcaa aaccgcagac ctacgtcaac
     2161 gaaaccgggc gggcggtcgc ggggctggtc aaactccacc gtgtcgagca accgaacctg
     2221 ctcgtcgcgt tcgacgatct ggatctcgag ctgggcaccc tccggatgag gccgtttggc
     2281 gggtcctcgg gtcaccgcgg aatgcgctca ttgatcaacc atctcggtga ccagggattc
     2341 ccccgtttgc ggatcggcat cgggaggccg cgtggccggc tcgacccggc tggctttgtg
     2401 ttgtcgaact ttgcctccga cgaactggat ttggtctctg agacgctcga ccgagcgctg
     2461 ggctgcgtcc gccggttcct cgaggccggg atcgaaaagg cgatgaccga ctgcaacacc
     2521 cgcccggcgg aatgatcgaa ctttcctcgc tgctggatct gattcgtgcg ctcccccagt
     2581 atcgggagct gcgcgagacc ctcgccgccc ggccaacccg cggtcgccac gtcccgatgt
     2641 tggctgcggc ccggccaccg atggcggccg ccctggcctc cgaattcaat gtcccagtgc
     2701 tcatgatctg cgcccgggcg gaccgggtgg ccaccctcgc ccaagagctt ccgagttggg
     2761 ctccctccac cgaagtgctc accttcccag aaccggatcc gttgttctac gaggcgcttc
     2821 cctggggagt gcgcaaccgc cagctccgag tggaaaccct cgcccggctc acccacccgg
     2881 gaggtcctca gccgctgatc atcctgacct cggcccgcgc catcatgacc cggacgatct
     2941 ccccggagca gttcgcggcc catacccatg agctgatcgc gggctcgtac cttcagctgg
     3001 attccctact caaatccttg atcggctcag gttacgaacc ggaaagcatc gtgaccgagc
     3061 gcggccagtt cagccggcgc ggcggcatct tggatctgtg gccgacctca gccccggctc
     3121 cggtccggat cgaactcatc gccgacgaga tcgagtcgat ccggcgattc gacccggcaa
     3181 cccagcggtc ttcagacccc gtccaggggg tgcaggtcgg cccggctcgg gagggccttc
     3241 ccgcctatta ccagcagtcg aacgacctgc ctctcccgct cgccgaggcg caacccatcc
     3301 tggaattctt ccttccctgg atgactcccg acccgcagac tgtgctcgat ttcctccccg
     3361 ccgagactct ggtgatc
//
LOCUS       DATDPQ010000328         9234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1501038, whole genome shotgun sequence.
ACCESSION   DATDPQ010000328 DATDPQ010000000
VERSION     DATDPQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501038"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VI520_07380"
     CDS             <1..533
                     /locus_tag="VI520_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLF37739.1"
                     /translation="PRETAVYPNSEFGLGLLQIAMLVKAEVGLEVAAVDLGGWDTHFA
                     QGANEGHMAELLADLGQGLAALHADLVDFHSGLSVVVMTEFGRRVQENASLGTDHGHG
                     SLMLLLGGNVVGNKIHGRWPGLEQDQLFGPGDLAVTTDYRDVLSEVCGLRLNNPALSQ
                     VFPNYRPRQSGFVRRG"
     gene            complement(540..1544)
                     /locus_tag="VI520_07385"
     CDS             complement(540..1544)
                     /locus_tag="VI520_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HLF37740.1"
                     /translation="MDQPFTTAQVCGGIERYSQPAIMAWNAHSRQAEGTFLFARVAIL
                     MGWLGLWLAACTGGSGRESPVEAALPLQLPPAWTPTPNREPSPTRVPSPSPGPSSTPF
                     SSGPEVIGYSVAGRPLEVFRFGRGPVRRLIVAGIHGGYEWNTIQLAYELMDHLHSHPE
                     LVPRRISLFVLPALNPDGEARSHGRPGRANENGVDLNRNWPSSWQAEWPKDGCWNYLP
                     INGGAAPASEPEVEALLSFILEKRFDSIISYHSAALGIFPGGQPPLADAVRLAEAVAA
                     VTPYPYPPVDSGCLFTGQFADWAADQGIAAIDLELETHRNTDLEINLHVLEVFLNWRR
                     "
     gene            1594..3666
                     /locus_tag="VI520_07390"
     CDS             1594..3666
                     /locus_tag="VI520_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37741.1"
                     /translation="MALKRGETLHHRYRIESVLGQGGMGAVYRAYDATLKVEVAVKEN
                     LFTTEEFARQFYQEATILANLRHPNLPRVTDHFVIRGQGQYLAMDFIQGKDLRERIEG
                     DGLISEGEAVPWFMEVADALAYLHSRKPYSILHRDIKPGNIKIQPDGRAVLVDFGLAK
                     VDDKSESTTTGAKAMTPGFSPPEQYGTGRTDARTDIYSLGATMYACLTGAIPEDSLER
                     AMGREELTPLRQRNPRVSAALARAIEKALAVRPDERYHSMVEFAAALGSAAQASKPTQ
                     VRDYPYLDRTARVLNLDRTVRAPITSGFGDLPTRLPFEQKKPPRWPQVAIPLAVVAII
                     AILALPGLLEGAGLASSERTSQPAGTQESSILPEATSPPAGLPAPSPTSALPSTTPLP
                     TVNPSIALTPQPTPIGGGIGQIAFASDRSGLPQIYLINIDGTDLNPLTSMPDGACQPS
                     WGPDGLSLVFTSPCPDSAETYSGAGLWILDMTNLQPQPLLTTPGGDYDPAWSPEGERI
                     AFTSERLGRPQVHVMTIDNGQVENVSGIFAREVQPIWSPQGTALVFVSYRNGGAQVWL
                     MSDGGGNEQRFARGESPDTHPAWSHDGQLVVFQRSLSGVPRLIGARFDDLGIPDFRVC
                     QQSPYSSHPMVDPDFSPDDRWLVFETWPDGVNHDIAIMTTTCANVTILDSDPALDFDP
                     AWRPLPSP"
     gene            3670..4560
                     /locus_tag="VI520_07395"
     CDS             3670..4560
                     /locus_tag="VI520_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HLF37742.1"
                     /translation="MTARIIDGKSISAEIRAEVAAGVEALIGRGGPIPGLATVLVGAN
                     PASATYLRAKRSAAAEVGIVTFDHQLAGGSTQQEVESFVAELGEDRRVHGILVQLPLP
                     NGLGEQSVLSRIRIEKDVDGFHPINLGRLAMKGGDPLFVPCTPAGCLTLLKRAGARLE
                     GSRAVVLGRSNIVGMPVALLLIKHDATVTVCHSMTQGVREICHEADVLIAAVGQAELV
                     RGDWIKPGATVIDVGINQVEDPSTKRGFRIVGDVAFQEAMDVAGVITPVPGGVGPMTV
                     AGLLQNTLRAAEIAHDRSSA"
     gene            4797..6542
                     /gene="ade"
                     /locus_tag="VI520_07400"
     CDS             4797..6542
                     /gene="ade"
                     /locus_tag="VI520_07400"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HLF37743.1"
                     /translation="MGRRPADLVVRAGRWVCVQSGEILPGTDIAIAGERIAYVGDDAS
                     HTIGDHTILVEADGRHLVPGLLDGHMHVESSMLTITEFVRAVLPHGTTGMFIDPHEIA
                     NVFGLEGVRMMVEEAAIQPIHVWVQMPSCVPSAPGLETAGAALGPREVAEAIRWPGVI
                     GLGEMMNFPGVILGADSLHAEMAETRAAGKVIGGHYASQDLGLPFHGYAAGGPQDDHE
                     GTRLDDAVARIRQGMKVMMRLGSAWHDVAEQLRAITELGLDSRHFLLVTDDSHAATLL
                     EEGHMDRVVRHAVQNGADPMVAIQMATLNAAEHFGLATDIGQIAPGRYADLLLVADLK
                     TFAVDTTIAKGRLAAEAGELRLAPPEFEYPMQVRASVHLARALVPADFGLPVAANGRV
                     QANVIGVIENQAPTRHLRLSLNPVAGQLTTDLSQDLAKVALIERHRGSGRLQVGLVQG
                     FGFRVPCAVASTVAHDSHHLLVVGTDEASMAVAANELARVGGGQIVVREGTPIALVEL
                     PIAGLMSDEPAERVAGKTKQLLQAFRDCGCTLNNPNMQLSLLALVVIPELRISDKGLI
                     DVNRFEVVPVVEREP"
     gene            6539..7285
                     /locus_tag="VI520_07405"
     CDS             6539..7285
                     /locus_tag="VI520_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLF37744.1"
                     /translation="MSREPAPYRRTAEALAELLDRSQPGDPLPSEPKLAEQMGVSRAT
                     LRDAMRAFQGRGLIVRRQGVGTYVGPKPIEAGLEELVSIETLAAQVGLQVAMGGLSVE
                     RRAPSQPEVETLELQVGQQVVEIARVIQAAGKPVAYLIDVIAEGLIPDEAWGSNFRGS
                     VLDLLLARGDLPLEYSRTEINAVAADHDLAARMELPLGEVLLLFEASLVTRDGLIVDR
                     SRSYFLPGTFRFQVVRQVGAAVGVSRPMPV"
     gene            7300..8250
                     /gene="pyrB"
                     /locus_tag="VI520_07410"
     CDS             7300..8250
                     /gene="pyrB"
                     /locus_tag="VI520_07410"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HLF37745.1"
                     /translation="MRSFAGRDILSLKGFERAEYFRVFEVADRLAPIARDRRNSDLLA
                     KKILVTAFYQPSTRTRLAHEAAMLRLGGKVTGFADPKMTRAGDFYQESIKDTVHMLEY
                     YGDVIVMRHFQQGAPAEAARWSSIPVINAGDGWGEHPTQVLTDLYTVFKERGSLDGLH
                     FVLVGDMRMRTMHSISYALSQFDIQASYIAPPEMSLTPEFKKEIDGLNVRYREVEHID
                     QVIEQADVIYMEPVVQPDYTKSRDERAGEVGMTPANYQVTKALLKRAKPDCIVLHSLP
                     RMDELLPEVDDTGHARYWIEAFNGVVVRMALLSLVLGAME"
     gene            8254..9234
                     /locus_tag="VI520_07415"
     CDS             8254..9234
                     /locus_tag="VI520_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HLF37746.1"
                     /translation="MQTDLGGRDLIGDLDFSKEEVETVLEVAFDLKRKRALGEPHPYL
                     RDRTLAMLFFFSSTRTRGSFEAGMAQLGGHAAFIESKTTQIAHGDTEREIGEIFGRYF
                     DGIAIRHVEWGVGNRYLNRVAEASRVPVLNMQCEIYHPFQILADLMTIMEKKGPNLRG
                     KKLVVSWAYAASYLKPISVPQSLILQMPRFGLDVTLAHPPEFKLMPQILDQAREQARK
                     HGTAFEVVNDMREGFKAADIVYAKSWGPLLTTTDQDQGKRIQDQYSGWITDGEKLGLA
                     KPDAIYMHPLPADRNIEVTDEVLDGPQSAVFDEAENRLHAQKAVMALTMR"
BASE COUNT         1705 a         2881 c         2993 g         1655 t
ORIGIN      
        1 gaccgcgcga gacggcggtc tacccgaaca gcgagttcgg gcttggcctc ttacagatcg
       61 cgatgctggt gaaggctgag gttggactgg aggtggcagc cgtggacctg ggaggctggg
      121 atactcactt tgcccaaggg gccaatgagg ggcacatggc cgaattgctg gcggatctcg
      181 ggcaaggcct ggcggcgcta cacgccgatc ttgtggactt ccattccggg ctcagcgttg
      241 tggtcatgac cgagtttggc cggcgggtgc aggaaaacgc cagtctcggc acggaccacg
      301 ggcacggcag cctgatgctg ctgctgggtg ggaacgtggt cggcaacaag atccatggcc
      361 ggtggcccgg actggagcag gatcagctct tcggccccgg ggacctggcc gtgacgaccg
      421 actaccgcga cgtgttgagc gaggtctgcg ggctgcggct caacaacccg gcgctctctc
      481 aggtctttcc gaattatcgg ccgcggcagt ccggatttgt ccgccgggga tgaccccgat
      541 cagcgccgcc agttcaggaa gacctccagc acatgcagat tgatctccaa gtcggtgttc
      601 cggtgggtct ccaactcgag atcaatcgcc gcgatcccct gatcggcggc ccagtccgcg
      661 aactggccgg tgaacaagca gccggaatcg accggcggat acgggtaggg agtgactgca
      721 gccaccgctt cggcaagccg gaccgcgtcg gcaagcgggg gttgcccgcc cggaaagatt
      781 cccagcgccg cactgtgata gctgatgatc gaatcgaacc gcttctcaag gatgaatgac
      841 aggagcgcct ccacttccgg ctcggaagcc ggagcggcgc ctccattgat cggcaggtaa
      901 ttccagcaac catccttcgg ccactccgcc tgccagctgg acggccaatt tcgattcagg
      961 tcaactccgt tctcgttcgc ccggccggga cggccgtggg agcgggcttc cccatccgga
     1021 ttcaaagcag gcagcacgaa cagactgatc cggcgcggga ccagctcggg atggctgtgc
     1081 aggtggtcca tcaactcgta ggccagctgg atcgtattcc attcgtagcc tccatgaatg
     1141 ccggccacga tcagccggcg gaccgggccg cggccgaaac ggaagacctc cagtggccgg
     1201 cccgccacgc tgtagccgat gacctccggt ccgctgctaa acggggtcga actgggcccg
     1261 ggagatggag atggaacccg agtcggtgac ggctcgcggt tcggagtcgg agtccaggcg
     1321 ggcggcagct gcaaaggcaa ggcggcctcg accggcgact cccgccccga accgccggtg
     1381 caggcagcca gccacagacc gagccaaccc atcaggatcg caactcgagc gaacaagaaa
     1441 gttccttccg cctgtctaga gtgcgcgttc caggccatga tcgccggctg ggaataccgt
     1501 tctatccctc cgcagacctg cgccgtagta aaaggctggt ccatttgcta caatgaccgc
     1561 ggcctgcggc cacacgcgcc ggccagtttt gtcatggccc tcaaacgcgg tgaaaccctg
     1621 caccatcgct atcggatcga aagcgtcctg ggtcagggcg gtatgggggc cgtgtacagg
     1681 gcctatgatg ccaccctcaa agtcgaagtc gcggtcaaag agaacctctt taccaccgag
     1741 gaattcgccc gccagttcta ccaagaagcc acgatcctag ccaacttgcg gcatcctaat
     1801 ttgcctcggg tcaccgatca ttttgtgatc cgcggccaag gccagtacct ggcgatggac
     1861 ttcatccaag gcaaagatct acgcgagcga attgaagggg atggcctgat ctccgaaggg
     1921 gaggcggtcc cctggttcat ggaggttgcc gacgctctcg cctaccttca cagccggaag
     1981 ccgtattcga tcctgcaccg cgatatcaaa ccgggcaaca ttaagatcca accagacggc
     2041 cgggcagtgc tggtggattt tggcctggcc aaagtcgacg acaagtctga gtcaacgact
     2101 accggggcga aagccatgac gcccgggttc tcgcctccgg agcagtatgg caccggaagg
     2161 accgacgcgc ggaccgacat ctactcgctc ggggcgacga tgtatgcctg cctgaccggt
     2221 gcaatcccag aggactccct cgagcgagcc atgggccgcg aagagctgac cccgctccgc
     2281 cagcgaaacc cgcgggtcag tgcggccctg gcgcgggcaa tcgagaaggc gttggccgtg
     2341 cgtccggacg agcgctatca ctccatggtg gagttcgctg ccgcactggg ttcggcggct
     2401 caagcgagta agccgactca ggtgcgcgac tatccatatc tcgaccgcac cgcgcgggtt
     2461 ctgaatctcg accgcaccgt gcgggctccg atcacgtcag gctttggcga cctgcccacc
     2521 cgattgcctt tcgagcagaa gaaaccgccg cgctggcccc aagtggcgat cccgctggcc
     2581 gtggtggcga tcattgcgat cctcgcactt ccaggactct tggaaggcgc cgggctggcc
     2641 tcttcggagc ggaccagcca accagccggc acgcaggaat cgagcatctt gcccgaggcg
     2701 acttccccgc cggccggtct gccggcccct tcgccaacct cggcgctgcc ctccaccacc
     2761 ccacttccga ccgtgaaccc ctcgatcgct ctgacgcctc agccgacgcc aatcggcgga
     2821 gggattggcc agatcgcatt tgcctcagat cggagcggtc tgcctcaaat ctatctgatc
     2881 aacatcgatg ggacggacct gaatcccctg accagcatgc cggacggtgc ctgccagccg
     2941 tcctgggggc ccgacggtct atcgctagtc ttcacctctc cctgtccgga cagcgcggag
     3001 acctattccg gcgccgggct ctggattctc gacatgacaa atctccaacc ccagccgctg
     3061 ctgaccaccc cgggaggtga ttacgacccc gcctggtcgc cggaggggga gcggatcgcc
     3121 tttaccagcg agcggctcgg ccggccgcag gtccacgtga tgaccatcga caatggccag
     3181 gtggagaacg tgagtggaat ctttgctcgg gaagtccaac cgatctggtc tccccagggt
     3241 accgccctgg tcttcgtctc ttaccgcaac ggtggggcgc aggtgtggct gatgtccgat
     3301 ggtggcggaa atgagcagcg cttcgcccgt ggcgaatctc cggataccca tccggcctgg
     3361 tcgcatgatg gccagctagt cgtcttccag cgttcgcttt cgggtgtccc tcggctgatc
     3421 ggagcgcggt tcgatgacct cggaattccg gatttccggg tctgccagca gagcccctac
     3481 tcgagccatc cgatggtcga tccggatttc tccccggatg accgctggct ggtcttcgaa
     3541 acctggccag acggggtaaa ccacgacatc gccatcatga cgaccacctg cgcaaacgtc
     3601 acaattttgg attccgatcc agctctcgac ttcgacccgg cctggcgccc gttgcctagc
     3661 ccgtaaggga tgacagccag gatcattgat ggcaaatcca tctcggccga aattcgggcc
     3721 gaggtggctg ccggggttga ggccctgatc ggccgcggcg gaccgatacc cggcctggcg
     3781 acggttctgg tcggagccaa ccccgcctct gcgacctacc tgcgggccaa gcgctccgcg
     3841 gccgccgagg tcggaatcgt gacgttcgat caccagctcg caggaggatc cactcagcag
     3901 gaagtcgaat ccttcgtggc ggagttgggt gaagaccgcc gggtccacgg aatcctggtc
     3961 cagctgccgc tgccgaacgg cctcggtgag caatcggttt tgtcccggat ccgaatcgag
     4021 aaagatgttg acggtttcca tccgatcaac ctcggtcggt tggccatgaa aggcggcgat
     4081 ccgctcttcg ttccgtgcac cccggctggc tgcctgactc tgttgaagcg cgccggcgct
     4141 cggctggagg ggagccgggc ggttgtgctt gggcgttcga acatcgtcgg gatgccggtc
     4201 gcgctgttgt tgatcaaaca cgacgccacg gtcactgttt gccattcaat gacccagggc
     4261 gttcgtgaaa tttgtcacga agcagacgtg ttgatcgcag cggtggggca ggcggagttg
     4321 gtgcgggggg actggatcaa gcccggggca actgtcatcg acgtgggcat caatcaagtg
     4381 gaggatccct ccaccaagcg agggttccga atcgtcggcg acgtcgcgtt tcaggaagcg
     4441 atggacgtgg ccggcgtaat cacaccggtg cccggaggcg tcggtccgat gacggtcgct
     4501 ggcttgctgc agaacacgct gcgggccgcc gaaatcgctc acgaccgaag cagcgcctga
     4561 ctttggagtc tcgaactccg gcgactgtcg agctagagca ccgcgggttt agtttacata
     4621 agtgtcccag gcagtgacat tcggcacctt ccgggctgtt gtctgacaat tgaccggcta
     4681 ggagccgggt gttacactcg ccacaattcg ccctgtgatc tccaacacct cggggcgcaa
     4741 taagcttgaa cgctcggcca gaacagctcc aatcgaaaca gcttgttgag gtggcgatgg
     4801 gtcgccggcc cgccgacctg gtcgtacgcg cgggtcgctg ggtctgtgtc caatcggggg
     4861 aaatcctgcc tgggaccgac atcgccatcg ccggcgagcg gatcgcttac gtcggggacg
     4921 acgccagcca tacgattggc gatcacacga tcctggtgga ggccgacggt cgccatttgg
     4981 ttcccggtct gctggacggg cacatgcatg tcgagtcgag catgctgacg atcactgaat
     5041 tcgtgcgggc cgtgctgcca cacggcacca ctggaatgtt catcgacccg catgagattg
     5101 ccaacgtctt cggcctggag ggggtccgga tgatggtcga ggaggcagcc atccagccga
     5161 tccacgtctg ggtacagatg ccgtcctgtg ttccttccgc gcccggcctg gagaccgccg
     5221 gggctgctct cgggccgagg gaggtggccg aagcgatccg ctggcccggg gtcatcgggc
     5281 tgggggagat gatgaatttc cccggggtaa tcctcggtgc cgattcgctt cacgccgaga
     5341 tggcggagac gagggcagcc gggaaggtaa tcggcgggca ctatgcctcc caggacctcg
     5401 gccttccgtt ccacgggtac gcggccgggg gcccgcagga tgatcacgaa ggaacccgtc
     5461 tggacgacgc ggtcgcccgg atccggcagg gaatgaaggt gatgatgcgg ctgggctctg
     5521 cctggcatga cgtcgccgag cagctgcgtg ccatcaccga gttgggcctc gattcacgcc
     5581 acttcctcct ggtcacggat gacagccacg ccgcgaccct gctcgaggaa ggacacatgg
     5641 accgggtcgt tcggcacgca gtccaaaacg gagccgaccc gatggtcgcg atccagatgg
     5701 ctaccctcaa tgccgccgaa cacttcggcc tcgcgaccga catcggccag attgcgccgg
     5761 gacgctacgc cgacctgctg ctggtcgcag acctgaagac gttcgcggtt gacacgacga
     5821 tcgccaaagg tcggctggcg gcggaggccg gagagctgcg cctcgccccg cccgagttcg
     5881 agtaccccat gcaggtcagg gcttcggtgc acctggcccg cgcacttgtc ccggcggact
     5941 ttgggctgcc ggtcgctgcc aacgggcggg tgcaggccaa cgtcatcggg gtcattgaga
     6001 accaggcccc aacccgccac ctgcggctca gcctgaatcc ggtagccggc cagctgacga
     6061 ccgacctcag ccaggatttg gccaaagtgg cgctcatcga gcggcaccgc ggatccggcc
     6121 ggctccaggt tggcctggtt cagggttttg gctttcgggt tccttgcgcg gtggcctcaa
     6181 ccgtcgccca tgattcccat catctgctgg tggtcgggac ggacgaggcc agcatggcgg
     6241 tggccgccaa cgagctcgct cgggtgggcg gcggccaaat cgtggtccgg gagggcacac
     6301 cgattgcgct ggtcgagctg ccgattgcgg gcctgatgtc ggacgaaccg gcggagcggg
     6361 tggccggcaa gaccaaacaa ctgctgcagg ccttccgcga ttgtggctgc actctcaaca
     6421 accccaacat gcaattgagc ctgctggctc tggtcgtcat ccccgagctg cgcatttcgg
     6481 acaagggttt gatcgacgtc aaccgctttg aagtggttcc ggtcgtggag cgggagccgt
     6541 gagccgggag ccggctccct atcggcgaac ggcggaggcg ctggccgagc tcctggatcg
     6601 cagccagccc ggcgatccgc tcccctccga gcccaagctg gccgagcaga tgggagtctc
     6661 gcgggcgacc ctgcgggatg cgatgcgggc ctttcagggg cgcgggttga tcgtgcgacg
     6721 gcaaggggtg ggcacctacg tcggcccgaa gccgatcgaa gccggcctcg aggaactggt
     6781 cagcattgag accctggctg cccaggttgg gctccaagtc gcgatgggcg gtttatcggt
     6841 ggaacgacgg gcacccagcc agccggaagt ggagacgctt gagcttcaag ttggacagca
     6901 agtggtggag atcgcccgag tgatccaagc ggcaggcaag ccggtcgcct acctgattga
     6961 tgtcatcgcc gaaggtctga ttccagatga agcctgggga agcaacttcc gtggctcggt
     7021 gctcgacttg ttgctggcgc ggggcgacct tccactggag tattcccgca ccgagatcaa
     7081 tgcggtcgca gcagaccacg acctggcggc ccgcatggaa cttccacttg gcgaagtgct
     7141 gttgttattc gaggccagcc tggttacccg ggatggctta atcgtggatc gatcgagaag
     7201 ctacttcctg ccggggacct tccgctttca ggtcgttcgg caggtcgggg ccgcagtcgg
     7261 ggtgagccgg ccgatgccgg tctaggaggc atctattcga tgcgcagctt tgcaggacgc
     7321 gacattctgt ctcttaaggg atttgagcgg gccgaatact tccgggtgtt cgaggtggcc
     7381 gatcggctgg cgcccattgc ccgggaccgc cgcaactcgg atctgttggc caaaaagatt
     7441 ctggtcacgg ccttctacca acccagcacc cgcacccgcc tggcgcacga ggcggcgatg
     7501 ctcaggctcg gcggcaaagt caccggcttc gcggatccca agatgacccg ggccggcgac
     7561 ttctaccagg agtcgatcaa agacaccgta cacatgctcg agtattacgg tgatgtgatc
     7621 gtcatgcggc acttccagca gggggcgccg gccgaggcgg cccgctggtc gagcataccg
     7681 gtgatcaacg ccggggatgg ctggggagag catccgaccc aggtgctgac cgatctgtac
     7741 accgtcttta aggaaagagg cagcctggac gggctgcatt ttgtgctggt cggggatatg
     7801 cggatgcgaa ccatgcactc gatcagctac gcgttgagcc agtttgacat ccaggccagc
     7861 tatatcgctc caccggagat gtcgctgact ccggagttca aaaaggagat cgatggcctc
     7921 aacgtgcgct accgggaggt ggagcacatc gatcaggtga ttgagcaggc cgacgtgatc
     7981 tacatggagc cggtggtcca accggactat acgaagtccc gcgatgaacg ggcgggtgag
     8041 gtcgggatga ctccggcgaa ttatcaggtt accaaggcgc tgctcaagcg ggccaaaccc
     8101 gattgcatcg tgctgcattc gctgccgcgg atggatgagc tgctgcccga ggtcgacgac
     8161 accggccacg cccgctactg gatcgaagcc ttcaatgggg tcgtggtgcg aatggccctg
     8221 ctctccctgg tgcttggagc catggagtag gccatgcaga ccgacctggg cgggcgtgac
     8281 ttgatcggcg accttgattt cagcaaagaa gaggtcgaga ccgtgctgga agtcgccttc
     8341 gacctgaagc gcaagcgcgc cttgggcgag ccccacccct atctgcggga ccggaccctc
     8401 gccatgctgt tcttcttcag cagcacccgc acccgcggtt ccttcgaggc cggcatggcc
     8461 cagctgggtg gccatgcggc attcatagaa tccaagacta cccaaatcgc ccacggcgat
     8521 accgagcggg aaatcggcga gatcttcggc cgctatttcg atggaatcgc gattcgccac
     8581 gtcgaatggg gggtaggcaa ccgctatctg aaccgggttg ccgaggcgtc ccgagtgccg
     8641 gtgctcaaca tgcagtgcga gatctaccat cccttccaga tcctggcgga cctgatgacg
     8701 atcatggaaa agaaggggcc gaatctgcgg ggtaagaagc tggtggtctc ctgggcctac
     8761 gcggcttctt acctcaagcc gatctcagtg cctcaatccc tgatcttgca gatgccccgc
     8821 tttgggctgg atgtaactct ggcccacccg ccggagttta agctgatgcc gcagatcctg
     8881 gaccaggctc gcgagcaggc ccgaaagcat gggaccgcct tcgaggtggt caacgacatg
     8941 cgggaaggat tcaaggctgc cgacatcgtg tatgccaagt cctggggacc gctgctgacc
     9001 actaccgatc aagaccaagg caaacgaatt caggatcagt actcgggatg gatcaccgac
     9061 ggcgagaagc tggggctggc caagccggat gctatctaca tgcacccgct gccggccgac
     9121 cggaacatcg aagtcaccga cgaggtgctc gacggccccc agagcgcggt cttcgacgag
     9181 gccgagaacc ggctgcacgc ccagaaggcc gtaatggcct tgaccatgcg atga
//
LOCUS       DATDPQ010000329         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3882038, whole genome shotgun sequence.
ACCESSION   DATDPQ010000329 DATDPQ010000000
VERSION     DATDPQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3882038"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VI520_07420"
     CDS             <1..322
                     /locus_tag="VI520_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37747.1"
                     /translation="GYKVAAAVDTAVPDRSQRLVACMADRYGRATGLRFHPNSVTLDM
                     TEYHSFYEIHSQTPAAIIEVGFLFLDQGFLVEEPDRVARGIVDGLLCFVNNEPATLPL
                     ESLP"
     gene            319..1296
                     /locus_tag="VI520_07425"
     CDS             319..1296
                     /locus_tag="VI520_07425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLF37748.1"
                     /translation="MNRLSIADSQAAAAIHAAHQALARGDKATARRQARLAAKLAPHS
                     EEPWLYLAATSEPKAALAYLARALEINPRSKRARKAIRWTVRQLPPRSRKAALRELDV
                     QPLPFEALSPRRILSWRIALPALGLAVGIGLWLGGQPADAREPHSASAPLDKATLTPT
                     PTPTPTPTPTPTLTPTPTPTFTPTATLTPTRRPAVSQQYSMDPAELADEGRWIDVDLG
                     NQSVTAYEGETAVQSFVVSTGTAIHPTVTGQFRVYIKLASTTMAGPGYYLTGVPWVMY
                     FYRGYGLHGTYWHNNFGTPMSHGCVNLTIPDSEWLYNFAPLGTLVNVHP"
     gene            1339..>2248
                     /locus_tag="VI520_07430"
     CDS             1339..>2248
                     /locus_tag="VI520_07430"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLF37749.1"
                     /translation="MPDGLELSVPLSRREFVRLSATGLLALGVPSQWTRAAVEAEPGL
                     LGRVVDPTLDIFSRPSFASEKRKTLWRDDVFEIAAAWIGDPIPEHNRTWYEANGLGFA
                     HSASVQPVRDLPNLPLESIPFGGVLTEISVPHVNAHWRPRSDSELAYRFYYGMTFWVT
                     GVSQDVSLQKWYRIFDDKYTYVYYAPAYAFRPIPVSELIPISSEIHPSEKLVEVDLAH
                     QWVNCYEAGQLVFTTKVSTGRKFGQDLYATPAGEFETFRKRPSRHMAAGNLASGYDLP
                     GVPWVAYIDEEGVSFHGTFWHNDFGVP"
BASE COUNT          414 a          759 c          645 g          430 t
ORIGIN      
        1 cggttacaag gtcgccgcag cggtggatac tgcggttccg gatcgatcgc agcggctggt
       61 ggcctgtatg gccgaccgct acggcagagc cacggggctc cgcttccacc caaacagcgt
      121 gaccctggac atgaccgaat atcacagttt ttacgagatc cacagtcaga ctccggctgc
      181 aatcatcgag gtcggttttc tctttctgga tcagggattc cttgttgaag agcctgatcg
      241 ggttgcgcgg gggatcgtgg atggcctgct ctgttttgta aacaacgagc cggccactct
      301 tccgctcgag agcttgcctt gaaccggctt tcgatcgctg attcacaagc cgccgcggcc
      361 atccacgccg cccatcaagc cctcgcccgg ggagataaag ccactgcccg ccgccaggcc
      421 cgcctggcag ccaaacttgc tccccactcc gaagagccat ggctctacct ggcggcaacc
      481 tccgagccaa aggccgcact tgcctacctg gcccgggccc tcgaaatcaa tccgcgcagc
      541 aagcgggccc gtaaggcgat ccgctggacg gtgcggcaat tgccgccgcg cagccgcaaa
      601 gccgcgctgc gtgaactaga cgttcagccg ctcccgttcg aggccctcag cccgcgccga
      661 atcctctcct ggcggatcgc attgccggcc ctgggattgg cggtggggat cggactctgg
      721 ctgggcggcc agccggccga tgcccgcgag ccgcattcgg cttcggcccc tctcgacaag
      781 gccactctaa ctccgacccc gacgccaact cctaccccga ccccaactcc gaccctcact
      841 cctaccccga ccccgacctt tactccgacc gcgaccctca ccccaacccg gcggccagcg
      901 gtctcgcagc agtacagcat ggatccggcg gagctggcgg acgaaggccg ctggatcgac
      961 gtggacctcg gcaaccagag cgtcaccgcg tacgagggag aaaccgcggt ccagagtttt
     1021 gtggtgtcca ccggcacggc aatccatccc accgtgaccg ggcaattccg ggtctacatc
     1081 aaactggcct ccaccacgat ggcaggccct ggctactacc ttaccggtgt gccgtgggtg
     1141 atgtacttct accgtggcta cggtctacat ggtacctatt ggcacaacaa cttcggcacc
     1201 cccatgagcc acggctgtgt caacctcact atccccgatt cggagtggct atataatttc
     1261 gcccccctcg ggacgctggt caacgtacat ccctagcggt ccgattcttg cagacattgc
     1321 gggggaaggg gtaattccgt gccggacgga ctcgagctta gcgttccact ctcacgacgg
     1381 gagttcgttc gcctgtctgc cactggcctg ctggccctag gggttccctc ccagtggacc
     1441 cgggccgcgg tcgaggccga gccgggcctc ctcggtcgag tggtcgatcc gactctcgat
     1501 attttcagcc ggccctcctt tgccagcgag aaacgcaaga ccctatggcg ggacgacgtc
     1561 ttcgagatcg ccgcggcgtg gattggagat ccaatccccg agcacaaccg cacctggtac
     1621 gaggccaacg ggcttgggtt tgctcattcc gcctccgtcc agcccgttcg ggacctgcca
     1681 aacctgcccc tggaaagcat tcccttcgga ggagtgctga cggagatctc cgtcccacat
     1741 gtcaatgcgc actggaggcc gcgatcggat agcgagctgg cataccgctt ctactatggg
     1801 atgacgttct gggtgaccgg ggtaagccag gatgtcagcc tgcaaaagtg gtatcggatc
     1861 ttcgatgaca aatacaccta tgtgtactac gcgccggcct atgccttccg ccccattccc
     1921 gtgtcggagc tgatcccgat ctcctcggaa atccatccga gcgagaaact ggttgaggtg
     1981 gacctggccc atcaatgggt gaactgctac gaggccggac agctggtatt cacgaccaaa
     2041 gtgtccaccg gccgaaaatt cggccaggac ctgtacgcta caccggccgg cgagttcgag
     2101 acgtttcgca aacggccctc ccgtcatatg gcggccggca acctggccag tggctacgac
     2161 ttgccggggg tcccgtgggt cgcctatatt gatgaagaag gggtctcttt ccacggcact
     2221 ttctggcaca atgattttgg cgtaccta
//
LOCUS       DATDPQ010000330         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2286810, whole genome shotgun sequence.
ACCESSION   DATDPQ010000330 DATDPQ010000000
VERSION     DATDPQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286810"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VI520_07435"
     CDS             complement(<1..556)
                     /locus_tag="VI520_07435"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HLF37750.1"
                     /translation="MITLAVQAGGRSSRMGRDKALLPLGGIPLIEHVLRRTAGLAEEV
                     LITTNRPADLVYLGVRLVADREPGAGALPGLLTALEAAHGETVLLLACDLPFVCRPLL
                     EHLLSLAHEAQVVVPQWKDNLEPLHAVYSNSCANAVRAAMQAGQPRVIDLLPRVTQRV
                     VAEAEIAQFDPDGSSFFNVNTPQDL"
     gene            601..1734
                     /gene="amrS"
                     /locus_tag="VI520_07440"
     CDS             601..1734
                     /gene="amrS"
                     /locus_tag="VI520_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HLF37751.1"
                     /translation="MRTSTLGDLLDPLTVEGKLYETQPDGALRCYACGHRCLIRPGKR
                     GICQVRFNQEGRLRVPWGYVGALQCDPTEKKPFFHLYPGSDTLTFGMLGCDFHCTYCQ
                     NWVTSQAMRDPASDISAAGLREVTPAELVEYGRKMGAWVIGSSYNEPLITSEWAVDVF
                     QVAREAGFTCVYISNGNTTPEVLEFLKPHVTGYKIDLKSMRDKTYRGLGGVLQHVLES
                     VERVHGLGFWLEVVTLVIPGFNDSSEELWDAARFLAGISPDIPWHVTAFHKDYKLIEP
                     DNTTAEILMRAAEIGQEAGLRYVYAGNLPGQVEDYENTYCPTCRTCLIERLGYVILAY
                     RLTEQGSCPSCGTRIPGVWPARAEQVRLGTSEDLYDRRPRPVI"
     gene            1745..>2040
                     /locus_tag="VI520_07445"
     CDS             1745..>2040
                     /locus_tag="VI520_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37752.1"
                     /translation="MEDSVKLLLTYDPLPKHREEYFRYVLGEFVPALENLGLTLCEAW
                     HTAYGEYPLRLTGFIAEDRETLDTVLGSPEFLELESRLQDFVINYSRKIVPQQPR"
BASE COUNT          389 a          622 c          652 g          377 t
ORIGIN      
        1 ccagatcttg tggcgtgttg acattgaaga aactcgatcc gtccggatcg aactgggcga
       61 tctcagcttc agctacgacc cgttgagtta ctctcggcag gaggtcgatc acccgcggct
      121 ggccggcctg cattgccgcc cggaccgcat tggcacacga gttggaatag accgcgtgca
      181 gcggctctag attgtccttc cactgcggga ccacgacttg cgcttcgtgc gccaggctga
      241 gcaagtgctc cagcagcggc cggcagacga agggcaggtc gcaggccaac agcagcacgg
      301 tttcaccgtg ggcggcctct agcgcggtca acagcccggg cagcgcgccg gcgccgggct
      361 cgcgatcggc caccagccga acgccgagat aaacgaggtc cgccggccgg ttggtggtga
      421 tcaggacttc ctcggcaaga ccggccgtgc gccgaagtac gtgctcgatc agcgggattc
      481 caccgagcgg gagcagcgcc ttgtcgcggc ccatccggct ggagcggcct cccgcctgca
      541 cggccagggt gatcacgcgg gcattctacc ttcggatgat ataatcctcc gacctgcgca
      601 atgcgcacca gcacgctggg agatctgctc gacccgctga cggtcgaggg gaagctgtat
      661 gagacccagc cggacggagc gcttcggtgt tatgcctgtg ggcaccgctg cctgatccgt
      721 ccgggtaagc ggggaatctg ccaggtccgt ttcaatcagg aagggcggct gcgagtcccc
      781 tggggctacg tcggcgccct tcagtgcgat cccaccgaaa agaagccctt cttccatctc
      841 taccccggct ccgacaccct gaccttcgga atgctggggt gcgacttcca ctgcacatac
      901 tgccaaaact gggtgacctc tcaagcgatg cgcgacccgg cctccgacat ctcggcggcc
      961 ggtctgcgcg aagtgacacc ggccgagcta gtggagtatg gccggaaaat gggcgcttgg
     1021 gtgatcggct cgtcgtacaa cgagccgctg atcacctccg aatgggcggt ggatgtgttc
     1081 caggtcgcgc gggaagccgg gttcacttgc gtttacatct ccaatgggaa caccactccc
     1141 gaagttttgg agttcttgaa gccgcatgtc accgggtaca agattgacct caagtcgatg
     1201 cgggacaaga cctaccgagg gctggggggt gtgctgcagc atgtgctcga aagcgtcgaa
     1261 cgagtgcatg ggctgggctt ctggctggaa gtggtgaccc tggtgatccc cggcttcaat
     1321 gacagttcgg aggagctatg ggatgcggcg cgtttcttgg ccggaatctc cccggacatc
     1381 ccgtggcatg tcaccgcatt ccataaagac tataagttga tcgaacccga caacacgacc
     1441 gccgagatcc tgatgcgggc cgccgagatc ggccaggaag ccgggttgcg ctacgtgtac
     1501 gccgggaatc tgccgggcca ggtcgaggac tacgaaaaca cctactgtcc aacctgccgg
     1561 acttgcctga tcgagcgact ggggtatgtt atcctggcct accgcctgac cgagcagggc
     1621 agctgtccga gctgcggaac acggatcccc ggcgtgtggc cggcccgggc tgagcaagtc
     1681 aggctgggga cgagcgagga cctgtacgat cgccgaccgc ggccggtgat atgaaaaact
     1741 aagcatggaa gattcggtca aactcctgtt gacttacgac cccctcccga aacatcggga
     1801 ggaatacttt cgctatgtat tgggggagtt tgttccggcg ctcgagaacc tcggcctgac
     1861 gctttgcgag gcctggcaca cagcctatgg cgagtacccg ctccgcttga ccggtttcat
     1921 cgccgaagac cgggagaccc tggatacggt gctgggttcg ccggagttct tggagttgga
     1981 gagccggctc caggactttg tcatcaacta cagccgaaag atcgttcccc aacaaccgcg
//
LOCUS       DATDPQ010000331         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3576021, whole genome shotgun sequence.
ACCESSION   DATDPQ010000331 DATDPQ010000000
VERSION     DATDPQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576021"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..537
                     /locus_tag="VI520_07450"
     CDS             25..537
                     /locus_tag="VI520_07450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLF37753.1"
                     /translation="MTSPRFSAPPAMQIDPALSYQATIRTDRGEIVLELWADRAPRTV
                     NNFVFLARNRFYDGVTFHRVIKNFMVQGGDPTGTGSGGPGYQFEDEFHPELRHDAPGV
                     LSMANAGPNTNGSQFFITHVATPWLDDKHSVFGRVTSGMQALLAIPERDPSKRNSPAV
                     TIQSIEIRES"
     gene            534..1097
                     /locus_tag="VI520_07455"
     CDS             534..1097
                     /locus_tag="VI520_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HLF37754.1"
                     /translation="MNQAAGPLPRRITALRVFALFAVIAITVFIYSIRDQAEQLEAYG
                     YPGIFLLSLLSNATIILPAPGIAITFAAGGVFDPFWVGVAAGTGAALGELTGYLAGYS
                     GRGVLEGTAIYQRLLELTHRFGNWTILVLAVIPNPFFDVAGAAAGALRMPVATFLLWA
                     TIGKLIKMWAVAYAGELSIDWVLRLLG"
     gene            complement(1049..2221)
                     /gene="menC"
                     /locus_tag="VI520_07460"
     CDS             complement(1049..2221)
                     /gene="menC"
                     /locus_tag="VI520_07460"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HLF37755.1"
                     /translation="MTGGNLWIEAAELRHVRMRLREPFETSFGVEHDRECLLIRIEGA
                     GQAGWGECVASGFPGYSYETVGTAWHVLEEFLIPAILHQPLVGISGLRAQIASVRGHP
                     MAKAGLELALWDWMGRVANLPLVDLLGGRRTRVQVGVSIGIQSGVDTLLKVVESYLRQ
                     GYPRIKLKIKPGHDLEPVRAVRQSFPRILLQVDANGGYDPGKFEQLLPLDEFELLLLE
                     QPFAEEQLLAHARLQERLATPICLDESIGSEADAQQALDLGACRIINIKPGRVGGLSE
                     AVAIHDVCRKADIPVWCGGMLETGVGRAANLALATLPGFSLPGDISASNRYYEEDIAS
                     PKFVLNSDGTIDVPTGAGLGITIDSGALERVTVRRERFTAGQPSSRSTQSIESSPA"
     gene            complement(2221..3096)
                     /locus_tag="VI520_07465"
     CDS             complement(2221..3096)
                     /locus_tag="VI520_07465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF37756.1"
                     /translation="MTLVIRPAFGPADMAQIEDLQREVWPGSETDVVPLHLLLTIAQN
                     GGVVLVASEGERIVGFVMGFLGTDEDSPDRVAMARLKHCSHMLGVHPDFRDRGVGLEL
                     KQAQRLAVIEQGIRLMTWTYDPLMSGNAHLNIHRLGAVSRIYKREVYGPMRDELNQGV
                     HSDRLQVDWWVTSNRVESRLQGSRPPLDLAHYLSAGAQRLNSAGLGEDGLARPGAGAE
                     TPTLNLALVEIPPDFQRLKRADLGLAAEWRAFTRVIFETAFNNGYVVTDFVHLKGEKF
                     PRSYYVLSLGEATLG"
     gene            complement(3093..4031)
                     /locus_tag="VI520_07470"
     CDS             complement(3093..4031)
                     /locus_tag="VI520_07470"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF37757.1"
                     /translation="MVLVTGGTGFIGQTVIRRLAEAGRQIRILLRPSQHSPNLPQGIA
                     VEVAIASMLDKRGVRAALVGVESVVHLASAEAHGREPELQAVDIQGTAVLAAAARQAK
                     VDRLIFLSHLGADLSSAFPVLRAKAIAEQSIQRSSVPATILRTSLAYGPGDRFTSAIA
                     MLLSISPLVFPLPGNGTTALQPIWVEDLATCILWSLEEPAMAGQTYEIGGPEFLTIEQ
                     LTQLIRRVTGTRRWVVHTSPVSVRALAWMMQRLLPRSPLTTFALDYLATSRMASLDAL
                     PRIFGLKPAILEQKLGYLRGRNWGVELLRRQLGWRR"
BASE COUNT          777 a         1370 c         1257 g          769 t
ORIGIN      
        1 aacgatagat aggagggtat tgaattgact tcgccacgct tcagcgcacc gcctgcaatg
       61 caaatcgacc cggccttgag ctaccaggcc acgatccgaa ccgatcgagg tgagatcgta
      121 ttggaactat gggccgatcg ggcgccgcgt acggtgaaca atttcgtatt cctggcccgc
      181 aaccggtttt acgatggggt gaccttccac cgagtgatca agaacttcat ggtccagggc
      241 ggcgatccaa ccgggaccgg gagcggtggg ccaggctacc agttcgaaga cgagtttcat
      301 ccagagctgc gccacgacgc cccgggcgtg ctttcgatgg cgaatgccgg tccgaacacg
      361 aatggttccc aattcttcat tacccacgtc gccaccccct ggctggacga caaacactcg
      421 gtctttggcc gggtgacctc tgggatgcaa gctttgctgg ccatcccgga acgcgatccg
      481 tccaagcgca attcaccggc cgtcacgatc cagtccatcg agatccgcga gtcgtgaacc
      541 aggccgccgg acccctgccg cgacggatca ccgctctgcg ggtatttgcg ctgtttgcgg
      601 tgatcgcgat cacggttttc atctactcga tccgtgatca ggccgaacaa ctggaggcct
      661 acggatatcc ggggattttt ctgctctcac tgctctcgaa cgcgacgatc atcctccccg
      721 cccctgggat cgcaatcacc ttcgcggccg gcggagtatt cgatccgttt tgggttgggg
      781 tcgccgccgg gaccggcgca gcgctaggag agctcaccgg ctacttggcc gggtacagtg
      841 gacggggtgt tttggagggg acggcgattt atcagcggct tttagaactc acccaccgct
      901 tcggcaactg gaccattctc gtactggctg tcatccccaa tcccttcttc gacgttgccg
      961 gggcggcggc cggagctctg cggatgccgg ttgctacctt cttgttgtgg gccacaatcg
     1021 gcaagctgat caagatgtgg gccgtggcct acgccggtga gctttcgatc gactgggtgc
     1081 tgcggctgct gggttgaccg gcggtgaaac gctcccggcg caccgtcact cgttcgaggg
     1141 ctcccgaatc aatcgtgatc cccaggccgg cgccggtcgg cacatcgatt gtaccgtccg
     1201 agttcaatac gaatttgggc gaggcgatat cctcttcgta gtaccggttg ctggctgaga
     1261 tgtcgccggg caagctaaac cccggcaaag ttgccagcgc caggttcgcc gcccttccca
     1321 cccccgtctc caacatcccg ccacaccaga ccggaatatc cgccttccga cacacgtcgt
     1381 ggatggcaac cgcctcgctc aaccctccga ccctgccggg cttgatattg ataatccggc
     1441 aggcaccgag atccagggct tgctgcgcgt cggcctccga gccgatgctt tcatccagac
     1501 agatcggggt agccagccgt tcctgcagcc gggcgtgcgc gagcagctgc tcctcagcga
     1561 agggctgctc gagcagcagc aattcaaatt catccagtgg gagcaattgc tcaaactttc
     1621 ccgggtcata cccgccgtta gcgtcaacct gaagaagtat cctggggaac gactgccgca
     1681 ccgcgcgcac cggctcgagg tcatggcccg gcttgatctt cagcttgatc cgagggtatc
     1741 cctgcctcag gtaggattcg acaaccttga gcagtgtatc cacacccgat tgaatcccaa
     1801 tggaaacccc gacctggact cgggtccgcc ggcctccgag caggtcgacc agcggcaagt
     1861 tcgccacccg acccatccaa tcccacagtg ccagttcaag cccggctttc gccatcgggt
     1921 ggccacgaac cgacgcgatt tgtgcacgca ggccggagat ccccaccaga ggctggtgga
     1981 ggatggcggg aatcagaaac tcctcaagca catgccaggc cgtgccgacc gtctcgtagg
     2041 agtagccggg gaagccgctg gcgacgcatt ccccccaccc cgcctgcccg gccccttcga
     2101 ttctgatcag caggcactcc cggtcgtgct cgaccccgaa ggaggtctcg aatggctccc
     2161 gcaaccgcat ccgaacgtgg cggagctccg ccgcctcaat ccacaagttg ccgcctgtca
     2221 ttagcccagg gtggcctcgc cgagcgagag cacgtagtac gagcggggga acttctcgcc
     2281 tttcaggtgc acgaagtcgg taaccacgta gccattgttg aaggcagtct cgaagatcac
     2341 ccgggtgaac gctcgccact ccgcggccag gccgaggtcc gcccgtttga gccgttggaa
     2401 gtccggcgga atctcgacca gggccaggtt gagggtcgga gtttcggcgc cggcgcctgg
     2461 gcgggccaga ccgtcctcgc caagcccggc cgagttgagt cgctgggcac cggccgagag
     2521 gtagtgagcc aggtcaagcg gaggccggga gccctgcagg cgggactcga cccgattcga
     2581 agtcacccac caatcgacct gcagccggtc ggagtggact ccttgattga gctcgtcccg
     2641 catcggcccg tagacctccc gtttataaat ccggctcacg gcgcccagcc gatggatgtt
     2701 caggtgggca ttcccgctca tcaacggatc gtaggtccag gtcatcaatc gaattccctg
     2761 ctcgatcacg gccagccgct gggcttgctt gagttcgagc ccaacccccc ggtcgcggaa
     2821 atccgggtgg accccaagca tatgggagca gtgtttcagc cgggccatcg cgacccggtc
     2881 cgggctgtcc tcgtcggtgc ccaggaaacc catcacgaaa ccgacgatcc gctcgccctc
     2941 cgaggcgacc agcacgacgc ctccgttctg ggcaatggtc agcaggagat gcaatggaac
     3001 gacgtcggtc tcgctcccgg gccagacctc ccgctgcaaa tcctcgattt gggccatgtc
     3061 ggccggacca aacgccggcc ggatcaccag agtcatcgcc gccacccgag ctgccgtctc
     3121 agcagctcca ccccccagtt gcgaccccga agatagccaa gcttctgttc cagaattgcg
     3181 ggcttgaggc cgaagatgcg aggcagggca tccaggctcg ccatccggct ggtcgccagg
     3241 taatcgagcg caaaagtggt cagcggtgag cgcggcagca gccgctgcat catccaggcc
     3301 agggcccgca ccgagaccgg cgaagtatgc acgacccagc gtcgggtgcc agtgactcgc
     3361 ctgatgagct gagtgagttg ttcgattgtc aagaactcgg ggccgccgat ctcgtaggtc
     3421 tgcccggcca tcgccggctc ctccagcgac cacaggatgc aggtcgccaa atcttcgacc
     3481 caaatcggct gcagtgcagt cgtcccgttc ccgggcaatg gaaagaccag cggcgagatc
     3541 gaaagcagca tcgcaatcgc actggtgaac cggtcccccg ggccgtaggc gagcgaggtc
     3601 cgcaagatgg tggcaggcac ggagctgcgc tggatggact gttctgcgat cgccttggcc
     3661 cgcagcactg gaaatgccga agagagatcc gccccgaggt ggctgaggaa aatcagccgg
     3721 tcgacctttg cctggcgggc ggccgcagcc agcacggcgg tcccttggat gtcgacagcc
     3781 tgcagttcag gctcccggcc gtgagcctct gcgctagcca gatgaacgac gctctcgact
     3841 cccaccaggg ctgctcgcac tcctcgcttg tccagcatgc tggcaatggc cacctccacg
     3901 gctatccctt gtggtaggtt cggtgagtgt tgactggggc gcagcagaat ccggatttgg
     3961 cgcccggcct cggcgagtcg ccggatgacg gtctgcccga tgaaccccgt tcctcccgtt
     4021 accaggacca ctaatctgga ctagccaatc ctcgacataa gggtgtaatt ctgccttccg
     4081 agcggggaag ccggatttta tcatggcgcc cgcgccgcct tcacaaccac agtgttatac
     4141 tcctggatcc ttgccgctgg aggaattgtt gag
//
LOCUS       DATDPQ010000332         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2291105, whole genome shotgun sequence.
ACCESSION   DATDPQ010000332 DATDPQ010000000
VERSION     DATDPQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291105"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VI520_07475"
     CDS             complement(<1..491)
                     /locus_tag="VI520_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HLF37758.1"
                     /translation="MANPKRKRVQDDSLEQDDEQELEEGLGSDEALDTVEGEVVDVEP
                     DFDESESSDAEQVLAELPETGPLSMPADGTDDPVRLYLKEIGLVELLSPDQELWLAVR
                     MAAPARLDGGIHAEVHQSEASSIQAAHLAMFENMRIAWKRLEEDARRSKESAPDLILI
                     VEEA"
     gene            complement(497..2365)
                     /gene="dnaG"
                     /locus_tag="VI520_07480"
     CDS             complement(497..2365)
                     /gene="dnaG"
                     /locus_tag="VI520_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLF37759.1"
                     /translation="MSVVEEVKDRTDIVEIIGQTVRLKKSGKNHTGFCPFHPNTRTPA
                     FVVFSETGTWRCFGACNEGGDVYKFLMKKEGWDFPQTLRYLADRAGVALETRSSEQEA
                     QEEAAAGLRDLLETAATYYRNNLLHSPPVLEYLHNRGLQDPTLESFGIGYALPGWEST
                     TSYLTGKGHRQPDLVEAGLAIAREAGGHHDRFHNRIMIPIRDARGRITGFGARAFDPK
                     DEPKFLNSPQSGLFDKGRLLFGLDKAGKAIRTAEQATVVEGYLDVLALHQAGYQNVVS
                     PMGTALTADQLRILKRYSRNIVLALDADTAGDRATLRGLTIAREAMDRESDPVFDSRG
                     LVRHEGRLDADIRIVSLPQGKDPDELLAEDPGSWPLLVAAAKPVVEYVMDRLLADLDL
                     EDAKAKAQVARRVLPLIEDVADSVEREAYRQSLARRLKLDERVLLGLRSPPGRAGRSS
                     EPPELPPIFRTRWTAAERFCLGLLLRDPELTYWVDRKLHALELEGLSPLDFYSTDSQI
                     IFQALRSSLAQVDEEPSQYWRSVLDGSTLAQAISIVDQVEQVDFDRPRVVETVSADFL
                     RLRKRRVESLLEQLSFQQQTVQESEEPADQMRYLTQEAQQLSSQKRRLEAALAARS"
     gene            complement(2459..2875)
                     /locus_tag="VI520_07485"
     CDS             complement(2459..2875)
                     /locus_tag="VI520_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37760.1"
                     /translation="MLAEQGYNPHGAALAVEDLAAWVSRPDWPKILQAYARCVRITRD
                     IPGLPSFSPDRIVEPATRELHAAILAGEQQPRRTGSVADFVEAFLPVVPAIDRFFDEV
                     LVMTDERALRENRLALLQRVVKLADGVADLSKLEGF"
     gene            complement(2966..>3434)
                     /locus_tag="VI520_07490"
     CDS             complement(2966..>3434)
                     /locus_tag="VI520_07490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HLF37761.1"
                     /translation="SRRIERLVERLADPFELTTSERGTALRAARLCKADLATQMVKDM
                     TSLQGAMGRYYALQSGEPQAVAEAILEHYQPRTTGDELPKSGPGVVLAVADRLDSLLG
                     LFAIGVQPTGTRDPYAMRRAALGVVQILTAQRQNFDLRIGLGWALEGLGRKAS"
BASE COUNT          605 a         1133 c         1040 g          656 t
ORIGIN      
        1 gcttcttcca caatcagaat cagatctggg gccgactctt tgctccggcg ggcgtcctcc
       61 tccagtcgct tccatgcgat ccgcatgttt tcaaacatcg ccaggtgggc ggcctggatt
      121 gaacttgctt ccgactggtg gacctccgca tggatcccac cgtccagccg ggccggggcc
      181 gccatccgca cggccagcca cagctcttga tccggactga gcagctcaac cagtccaatc
      241 tctttgagat agaggcggac cggatcatcc gtgccatcgg ccggcatgga gagcggcccg
      301 gtctccggca gctcggccag gacctgctcg gcgtcgctgg actcgctctc gtcaaagtcc
      361 ggctcgacgt cgactacttc cccttcgacg gtgtctaggg cctcgtccga ccccaggcct
      421 tcttcgagct cttgttcgtc gtcttgctcg aggctgtcgt cttgcacgcg ttttcgcttc
      481 gggttggcca tggaggctag cttcgggctg ctagcgccgc ctccagccgg cgtttctggg
      541 agctcagctg ttgggcttcc tgggtcagat aacgcatctg atcggcgggc tcttcggatt
      601 cctgcaccgt ctgttgttgg aaacttagtt gttccagcag actctccacc cgccgcttgc
      661 gcaatctcag gaaatccgcc gataccgttt caaccacccg cggccggtcg aagtcgacct
      721 gctcaacctg atcgacgatc gagatcgcct gggccagggt cgagccgtcc agaacggatc
      781 gccagtactg gctcggctcc tcatcgacct gggccaggga ggaccgcagg gcttgaaaaa
      841 tgatttgcga gtcggtcgaa tagaagtcca gcggcgaaag cccctccagc tccaaggcat
      901 gcagcttgcg gtcaacccag taggtgagct ctggatcccg caacaataac cccaggcaga
      961 accgctcggc cgcggtccag cgggtgcgga atatcggagg cagttcgggc ggctcagagc
     1021 tgcgcccggc gcggccggga gggcttctca gtcctaagag gacccgctcg tccagcttga
     1081 gcctgcgggc caggctctgg cgataagcct cccgctcaac cgagtccgcc acatcttcga
     1141 tcagcggcag cacccgccga gcgacctggg ccttcgcttt tgcatcctcg agatccagat
     1201 ctgccagcaa ccgatccatg acatactcaa ccaccggctt cgctgcggca accagcaatg
     1261 gccagctgcc ggggtcttcg gcaagcagct catccggatc cttgccctgc ggcaaggaga
     1321 cgatccgaat atcggcgtcc aggcgtcctt catggcgcac cagcccgcgc gagtcgaaca
     1381 ccggatccga ctcgcggtcc atcgcctccc gggcgatcgt caggccgcgc agggtggcgc
     1441 ggtctccggc ggtgtcggca tccagcgcca agacaatgtt gcggctgtag cgcttcaaga
     1501 tccgaagctg gtcagcggtc agggcggtgc ccatcggcga aaccacgttt tggtacccgg
     1561 cctgatgcag agccaggaca tccaggtagc cttcaaccac ggtcgcctgc tctgccgtcc
     1621 ggatggcctt gccggccttg tccaatccga agagcaggcg acccttgtcg aacaggccgc
     1681 tctgaggcga gttgaggaac ttgggttcgt ccttcggatc gaacgcccgt gccccaaatc
     1741 cggtgatccg gcctcgggcg tcccggattg ggatcatgat ccgattgtgg aagcggtcgt
     1801 ggtgtccgcc agcttcccgg gcgatggcca ggccagcctc gaccaggtcc ggctggcggt
     1861 gccccttccc ggtcaggtaa ctggtcgtcg attcccagcc ggggagggca tagccgatcc
     1921 cgaaactctc caaagtcggg tcttgcagac cccggttgtg caaatactcc agcacaggcg
     1981 ggctgtgcag cagattgttc cggtaatagg tcgcggccgt ctctagcaga tcgcgtaagc
     2041 cggccgccgc ctcttcctgt gcctcctgct ccgatgagcg ggtctccagc gccaccccgg
     2101 cccgatccgc caggtagcgg agtgtctggg ggaagtccca accttccttc ttcatcagga
     2161 acttgtagac atccccgcct tcattgcagg cgccaaagca gcgccaggtg ccggtctcgc
     2221 tgaagaccac gaaggcggga gtgcgggtgt tgggatggaa tgggcagaat ccggtgtggt
     2281 tcttcccgga cttcttcaag cggaccgttt ggccgatgat ctcgacaata tcggttcggt
     2341 ctttgacttc ctcaactacg ctcatcggtg actaacttcg ggcccgctca gactagagcg
     2401 gaggccggtt ttcaaacttc ccgggtcagg ttgggaatca tagccgaaat cctgctggct
     2461 agaaaccttc cagcttcgaa aggtcagcga cgccgtcggc tagtttcacg acccgctgca
     2521 gcagggccaa tcggttttcc cgcaaggcgc gctcgtcggt catcaccagc acctcatcga
     2581 agaaccggtc aattgcggga actaccggaa gaaaggcctc aacaaaatcg gccactgagc
     2641 cggtccggcg cggctgctgc tcgccggcca ggatggccgc atgcaactcg cgggtggcgg
     2701 gttcgacgat ccgatccgga gagaagctcg ggagacccgg aatgtcccga gtgattcgga
     2761 cacagcgggc gtaggcctgc aggatcttcg gccaatccgg ccggctgacc caagccgcca
     2821 agtcctcgac cgccagggcc gcgccgtgcg ggttgtagcc ttgctcggcc agcaccgctt
     2881 cgatgacccc gtgcggccac ccctttccca gcagcaggcg ctgctgtctg gcggagatga
     2941 aatccaggca gtccggcaga acctgctacg aggcctttcg gccgaggcct tccaaagccc
     3001 agccgagacc gatacgcaaa tcaaagtttt gccgctgagc ggtgagaatc tgtacaaccc
     3061 cgagggcggc ccggcgcatg gcatacggat ctcgagtgcc tgtcggctga accccgatcg
     3121 caaacaaccc gagtaggctg tcgagccgat ctgccaccgc caagactacg cccggcccac
     3181 tcttgggaag ttcatcaccg gtggtgcgcg gttgataatg ctcgaggatc gcctccgcca
     3241 ccgcttgcgg ttctcctgat tggagcgcgt agtagcgccc catggccccc tggagcgaag
     3301 tcatgtcctt gaccatttgg gtagccaggt cggccttgca cagccgggca gcccgaagtg
     3361 cggtccctcg ttcgctggtg gtcaattcaa agggatccgc tagccgctcg accagccgct
     3421 cgatccgtcg cgac
//
LOCUS       DATDPQ010000333         9192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1763474, whole genome shotgun sequence.
ACCESSION   DATDPQ010000333 DATDPQ010000000
VERSION     DATDPQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763474"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VI520_07495"
     CDS             complement(<1..202)
                     /locus_tag="VI520_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37762.1"
                     /translation="MHFGVLLLCGHLPSIGLVGVLMARMVLMARMGGMLLAGGVFVVV
                     FHRSILPKDSNGTDLAILPSGVT"
     gene            197..2296
                     /locus_tag="VI520_07500"
     CDS             197..2296
                     /locus_tag="VI520_07500"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HLF37763.1"
                     /translation="MHHAHAEHTAAPDRAAAAHEHHMAHEGPQVAPAHGVTTPEHSHH
                     ADHTGHEQMFRRRFWVNTLLSVPVLLFSPFIQERLGYQMAEFPGSGWITPVFAVVIFG
                     YGGLPFLRMARPEVRNRRPGMMMLISLAISVAFTYSLAALFIGGDTFFWELVTLIDIM
                     LLGHWLEMRSVRQASGALNELAKLLPDTAERIDPDGNTRVVAVSQLQLADLLLVRPGA
                     SVPADGEVEEGESDVNEAMLTGESRPVAKRPGDRLIAGSIIGDGSLRVRVRAIGEQTA
                     LAGIMRLVEQAQQSKSNTQILADKAAGWLFYIAIGVAGLTGVAWTAAVGFNADVIARV
                     ATVLVIACPHALGLAVPLVVAITTAMGANNGILVRDRLALEEARLIDTVIFDKTGTLT
                     RGEFGVVATATVDGLTADQALALTAAVEGDSEHTIAQGIRRSAEERQLVLPPTRNFEA
                     IKGRGVRGAVDGRQIHVGGPRLLEVLQLSLPEPIARFNEETSRKGQTVVYLVLDGKVA
                     AGFALADVIRPESKQAIERLHQMGVEVAMLTGDSQAVAKAVAEELAIDHYFAEVLPEH
                     KDQKVLELQRQGKRVAMVGDGVNDAPALTRADVGIAIGSGTDVAVESAGIILVKSNPL
                     DVVKIIALSRASYRKMIQNLIWATGYNVVALPLAAGVLASVGILLSPALGALFMSLST
                     VIVAINAQLLRRERIAA"
     gene            complement(2325..2513)
                     /locus_tag="VI520_07505"
     CDS             complement(2325..2513)
                     /locus_tag="VI520_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HLF37764.1"
                     /translation="MVKDPVCGMMIEPETAFTTREHAGNTLYLHAQECADKFDADPHR
                     YGHPDEHEPHGPHEHPTG"
     gene            complement(2639..3412)
                     /locus_tag="VI520_07510"
     CDS             complement(2639..3412)
                     /locus_tag="VI520_07510"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HLF37765.1"
                     /translation="MFPFLRLGPFLLQLPGLALLAGVWVGSSLADKEATRLKLKPGAI
                     SNLIFYALVAGVVGARLGYAARYISTYLANPLGLLTLTPATLLPLAGIVTGVAVAVLF
                     GRHKRLPLRPTLDALAPGLAAFLVGHGMANILSGDAFGSPTTVPWAIFLWDENRHPSQ
                     IYETLAALLVFAVEWKQPLGRPGAGVNFLLVVALSAAARVFLEAFRGDSLIWPGGFRA
                     AQVVGLVVLALSWRLMQLWRQPSVPRPRSDQRRQEAEAH"
     gene            complement(3412..3960)
                     /locus_tag="VI520_07515"
     CDS             complement(3412..3960)
                     /locus_tag="VI520_07515"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLF37766.1"
                     /translation="MSPSARLMAAMLALGGVWTFLSRVPPSATTGGAPPPSPREGFSA
                     PDFTLDLLGSGQLTLSDLRGKVVLVNLWASWCPPCRAEMPAIEEIYQAFQTQGLIVLG
                     VNTTFQDSESDAAAFIEEFGLSFPILLDREGRVSRRYQMRGLPSTFFIDRQGIIRTVV
                     IGGPMSEAVIQSKVEELLKQGG"
     gene            complement(3960..4085)
                     /locus_tag="VI520_07520"
     CDS             complement(3960..4085)
                     /locus_tag="VI520_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HLF37767.1"
                     /translation="MVALVAGLLSFLSPCVLPLVPAYVGYLSGHAIGSASSSTAA"
     gene            complement(4188..4727)
                     /locus_tag="VI520_07525"
                     /pseudo
     CDS             complement(4188..4727)
                     /locus_tag="VI520_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
     gene            complement(4997..5347)
                     /locus_tag="VI520_07530"
     CDS             complement(4997..5347)
                     /locus_tag="VI520_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HLF37768.1"
                     /translation="MEVTLQAAEFRFEPSRVEVMAGQQVTITMENMGTVEHDFVIQEI
                     PVEQSAAGSELATPGHTMDEMEVEPAVHMGAMAGMNSSVTFVPTKPGNYEFFCAIPGH
                     KEAGMVGTLDVREP"
     gene            complement(5406..5945)
                     /locus_tag="VI520_07535"
     CDS             complement(5406..5945)
                     /locus_tag="VI520_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HLF37769.1"
                     /translation="MSLTVVLAMHGAPANDFPSEELAELMGLHAALERATGERRQALE
                     SRHSELEAKLRTWPRTERNDPFWAGSHTLAEALREATGHEVIVGFNEFCAPSLDQALD
                     QAAARGMQEIVVVTPMMTRGGEHSEADIPGAIERAVRRHPAISFRYAWPFNPGEVARF
                     LAAQLEKVRTGRPDEPAKT"
     gene            6178..8598
                     /locus_tag="VI520_07540"
     CDS             6178..8598
                     /locus_tag="VI520_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLF37770.1"
                     /translation="METPEATAEITLDVRGMTCASCVAHVEGALTDLEGVTAAAVNLG
                     LGTARVGYVPGLVAIGQMKRAVREAGYEAQERSQGLDALDRERAAREGEIRRQGRNLA
                     IAGAVGMLVMVGTFYDMLGPLRSWIPAWLSYKWVIGLLTTPIVFGPGRQFFTNSWRGL
                     KHGVTDMNLLYATGIGAAYGIAVINTLFPKAGFGGEGATFFESAALLTAFVVLGRWLE
                     ALTRGRTSEAIRKLMKLQPKLARVLRGTTDEEIPASEVEVGDLLLVRPGESLAVDGLV
                     EEGYSAVDESMLTGESLPVEKKAGDSVIGGTLNKSGAFRFRATRVGRQTALAQIIKLV
                     EDAQASKAPIQKLADWVAGHFILGVHMLAAVVFVFWFFFGYEHYFDPNSSFILSPYRL
                     GEIGVFGFSLLLSVTVLVISCPCAVGLATPSAMMAGAGKAAEHGVLFKGAEAIENTSK
                     LQTLIFDKTGTLTRGEPSVTDVLARSSPPGQAESAWALRPDAILRFAASAELNSEHPL
                     GEAIVRGAQTRGIEILDPERFESIPGHGVEAEAEGRAVLLGNRRLMAERAIGISDWLG
                     EAERLEAEGKTVMFLAVDGHPAGLIAVADTLKDHSREAIQQLHRLGLEVAMITGDNLR
                     TAQAIARQVGIDRVLAEVLPQDKAEEVKKLQALGKRVAMVGDGVNDAPALAQADVGMA
                     IGSGTDVSKETGDVILIKDDLRDVVVALEAAKATMRKVKQNLFWAFFYNTLGIPVGAG
                     LLYPVIRLVVSPELAAALMALSSVSVTLNTLLLKRFKPSLRGGQPPQPEAQGAEQPRW
                     AGAPAGGD"
     gene            8614..8847
                     /locus_tag="VI520_07545"
     CDS             8614..8847
                     /locus_tag="VI520_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37771.1"
                     /translation="MERESVEREIARRSALIYLPISLGAALLFVLAASSAGEYPTVAR
                     VGGAIWVGLLSLIVSMPLVTARVKTRKRGQPES"
     gene            8854..>9192
                     /locus_tag="VI520_07550"
     CDS             8854..>9192
                     /locus_tag="VI520_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37772.1"
                     /translation="MLIAQRNLIQEKTRFALSVSGVALAIMLVLILDGFQVGMFRQIT
                     SYLDHSEGSLVVAQEGVVNLIGATSLLPLGTRAQVEAVEGVQGVAPILSQFVILDLHD
                     KKQPAYLVGYS"
BASE COUNT         1705 a         2883 c         3020 g         1584 t
ORIGIN      
        1 ttgtcacacc ggacggtaga atagccaggt cggttccgtt cgagtctttg gggaggatag
       61 atcgatggaa gaccacgaca aacacacctc cggcgagaag catcccgccc atgcgggcca
      121 tgagcaccat gcgcgccatg aggaccccca ccaggccgat gctggggaga tgcccgcaga
      181 ggagcaaaac gccgaaatgc accacgctca cgccgaacac acagctgccc cggaccgggc
      241 cgctgccgcg cacgagcatc acatggccca tgaggggccg caagtggcgc ctgcccacgg
      301 ggtaaccact cccgagcact cccaccacgc ggatcacact ggccacgagc agatgttccg
      361 gcgccgcttc tgggtgaaca cactgctctc cgttccggtg ctgctcttca gcccgttcat
      421 tcaagaacgg ctggggtacc agatggccga gttcccgggc agcggctgga tcacgcctgt
      481 cttcgcggtg gtgatcttcg gctacggcgg attgcctttc cttcggatgg cccggcccga
      541 ggtccgcaac cgccgaccag gcatgatgat gctgatctca ctggcgatca gtgtagcgtt
      601 cacctacagc ctggccgccc tgttcatcgg aggcgacacg ttcttttggg agttggtcac
      661 gctgatcgac atcatgctgc tcggccattg gctcgagatg cgcagcgtgc gacaggcctc
      721 cggcgcattg aacgagctgg ccaagctgct gcccgacacc gccgagcgga ttgatcccga
      781 tgggaacacc cgggtggttg ccgtttccca attgcagctt gccgacttgc tgctggtccg
      841 gcccggagcc agtgttcccg ccgacggcga ggtggaagag ggggaatcgg acgtcaatga
      901 ggccatgctc accggcgagt ccaggccggt ggccaagcgg ccgggcgatc ggctcattgc
      961 cggctctatc atcggagacg ggagcctgcg ggtccgggtg agggccatcg gggagcagac
     1021 cgccctggct ggcatcatgc gcctggtgga gcaggcgcag cagagcaagt ccaataccca
     1081 gatcctggcc gataaagcgg caggctggct gttctacatc gcgattggtg tggccgggct
     1141 gaccggcgtg gcctggacgg cggcggtggg attcaacgcg gacgtcattg cccgggtggc
     1201 gacggtgctg gtaatcgcct gtccgcacgc gcttggcttg gctgtccctc tggtggtcgc
     1261 catcaccacc gccatgggcg ccaacaacgg aatcctggtg cgcgaccggc tggcgctgga
     1321 agaggcccgg ctaatcgaca cggtcatctt tgacaagacc ggcaccttga cccgcggcga
     1381 gtttggggtc gtggccaccg ccaccgtgga tgggctgacc gcggatcagg ccctggcgct
     1441 caccgcggcc gtggagggcg actccgagca cacgatcgct caagggatcc gccggtccgc
     1501 cgaggagcgc cagttagtgc ttccgccgac tcgaaacttt gaggccatca agggccgcgg
     1561 cgtgcggggc gcggtggacg gtcgccagat ccacgtcggc gggccgcgct tgctcgaggt
     1621 gctccagctg agcctgcccg agccgattgc tcgcttcaac gaagaaacca gccggaaggg
     1681 tcagaccgtc gtctacctgg ttctggatgg caaggtagca gccgggttcg cgctggccga
     1741 cgtcatccgc ccggagagca agcaagcgat cgagcggctg catcagatgg gagtcgaggt
     1801 cgcaatgctc accggcgaca gccaggccgt agccaaagct gtcgccgagg aattggccat
     1861 cgatcactac ttcgccgagg tgctgcccga gcacaaggat cagaaggtac tggagctgca
     1921 gcggcagggg aagcgggtag cgatggtcgg cgacggagtc aacgatgcgc cggccctcac
     1981 tcgggccgat gtcggcatcg ctatcggcag cggtacggac gtcgcggtgg agtcagcggg
     2041 gatcatcctg gtcaagagca acccgctcga cgtggtgaag atcatcgcct tgagccgtgc
     2101 cagctatcgc aagatgatcc agaacctaat ctgggctacg ggttataacg tggtggcgct
     2161 accgttggcg gctggggtgc tggcgtcggt gggtatcttg ctctcgccgg ccctaggcgc
     2221 tttgttcatg tccttgagca cggtgatcgt agccatcaac gcccagctgc tgcgccggga
     2281 gcggatcgcg gcataaagat ctgttgggcc tggagcgatc gcacttatcc cgtcggatgt
     2341 tcgtgcggac cgtgcggctc gtgttcatcg gggtgcccgt atcggtgcgg atccgcgtcg
     2401 aatttgtccg cacattcctg ggcatgcaaa tagagggtgt tcccggcatg ctcgcgggtc
     2461 gtgaaggcgg tttccggttc aatcatcatt ccacagacgg gatctttgac catcgcttgg
     2521 atcctcctca gggttggggt tgagcaccaa gccgaattac tcagggcaag ggcttcatcc
     2581 tatcctgcac ccgttccagg cttacattgt ggcgcgctgc gcgtgcttgt tggcatgttc
     2641 aatgggcttc cgcctcctgc cttctctgat ctgatcgcgg tcgaggtacg cttggctgtc
     2701 tccacaattg catcagtcgc cacgacaaag ccaggaccac cagtccaacg acctgcgcgg
     2761 ctcggaagcc gcccggccag atcaggctgt cgcctcggaa tgcttccaga aagacgcgcg
     2821 ccgcggccga gagcgcgacc acgagcagaa agttgacccc agctcctggc cgaccgagcg
     2881 gctgcttcca ctcgacggcg aagaccaaca gcgccgcaag agtttcgtag atttgggaag
     2941 gatggcgatt ctcatcccac aggaagattg cccaggggac agtagtcgga gatccgaacg
     3001 catcgccgct caggatgttg gccatcccat ggccgaccag gaaggccgcc aagcccggcg
     3061 ccagcgcgtc cagcgtcggt cggagcggga gccgcttgtg tcggccaaac agcaccgcga
     3121 ctgcgacccc ggtgacgatt cccgccaagg gcaagagagt ggcgggtgtc agggttagca
     3181 gcccgagcgg gttggcgagg tatgtgctga tgtagcgggc cgcatacccc aatcgggcgc
     3241 caacgacccc ggccaccagc gcataaaaga tcaagttgga aattgccccc ggcttcagct
     3301 tgaggcgagt ggcctctttg tcggccaaac ttgacccgac ccagactccc gcaagcaggg
     3361 ccagcccggg gagctgcagc aagaaggggc cgaggcgcaa gaacgggaac actatccacc
     3421 ctgcttgagc agctcctcaa ccttggattg aatgacagcc tcgctcatcg gcccaccgat
     3481 gaccaccgtg cgaatgatgc cctgccgatc gataaagaag gtcgacggaa gcccacgcat
     3541 ctgataacgc cggctcactc taccctcgcg gtcgagcagg atgggaaagc tcaacccaaa
     3601 ttcctcaatg aatgctgccg cgtctgactc cgagtcctga aaggtcgtat tcaccccgag
     3661 gacaatcaat ccttgggtct ggaatgcctg atagatctct tcgatggccg gcatttcggc
     3721 gcggcagggc ggacaccagg aggcccagag gttgaccagc acaaccttgc cgcgcaggtc
     3781 ggacagcgtc agttgcccgc tgcccagcag atcaagcgtg aagtcggggg ccgagaaccc
     3841 ctcccgcggg ctgggtggcg gtgcgccacc ggtggtcgcg gagggtggga cccgggagag
     3901 aaaagtccag actccgccaa gtgccagcat tgcggccatc agccgcgcgc tgggagacat
     3961 catgctgcgg ttgagctcga ggcgctgccg atcgcgtgtc cgctgaggta gcccacataa
     4021 gctggcacga gcggcagcac gcagggcgag aggaacgaga gcagtccggc caccagagcg
     4081 accagatacg tcgggactac tgtgccgcct agtggcacgt caagaaacag accattgcgc
     4141 tgggcccaga ttgcgatgag ggtgatccgg ccgctcacca tcatcagtcc gatgacgatc
     4201 aggaacaatc cgctggcgat ctccacttta cggacgtact tccgaaagcg gcgcacaatg
     4261 acgatcgcgc gctccattcc caatgcgatc agaaggaagg gcagccccag ccccagcgcg
     4321 tagccgctgc tcagcaccat cgcctggccc gaaccctgtt gggtaaagcc aagggtgagg
     4381 atcgcgccca gggtcgcgcc gatgcagggt gtccaaccgg ccgcaaagaa tacgcccatc
     4441 agaccggagg ccagccaacc gccgcgttgt gtcgtgcccc actgcgggcg cgtatcgtaa
     4501 tacagccaag gaattctcag cacccgcaga gtggccaggc cgaacacgat caccagcacc
     4561 ccccctacct gtcctaagcc ggatttgtat gaaccaaaaa gctgacccag cgcagtggcc
     4621 gcgccacccc agccgatcac gaagatgagc gagaacccca gaacgaagag gaccgcgtgc
     4681 aggaagaggg tgaggcgacg gctcggcaat gtaccgctgc tagccacagg ggtctgatcc
     4741 tcgggatggt cgcatgaaca ccgttcggca agcgatcagc cacgcggtct cgctggaggt
     4801 ctgatgcagc ggcatggctc catcttacca aggccaggga cccagctcgc tgaaggcggc
     4861 gccgagccgt cagccaagag gccgctcggg gttgggtcga gctatgcgaa atagcgggct
     4921 gcgaaggggc gccgtttgtg aaacgcggaa ctaatggcag gctgcgagcc ggatcccggg
     4981 ttggccgacc gatgggctag ggttctcgga cgtcgagtgt cccgaccatt ccggcctctt
     5041 tgtgtccggg gatggcacag aagaattcat agttcccagg cttcgtcggg acgaaggtca
     5101 cgctggagtt catcccggcc atcgccccca tgtggaccgc tggctccacc tccatctcgt
     5161 ccatggtgtg gccgggcgtc gccagttcgg atcccgcagc cgactgctcc acggggattt
     5221 cctgaatcac gaaatcgtgt tccaccgtgc ccatgttctc catcgtgatg gtcacctgct
     5281 ggccggccat gacctccacc ctggacggct cgaagcgaaa ttcggcagcc tgcagcgtga
     5341 cctccatcgg caccggcccc gacgagcatg ccgcgagcag ggcggcgccg atagaagcac
     5401 caagactagg ttttcgcggg ctcatcaggc ctccctgttc ggaccttctc cagttgagcg
     5461 gcgaggaacc gggcaacctc gccgggattg aagggccagg cgtagcggaa gctaatcgca
     5521 ggatgcctcc ggaccgcgcg ctcgatggcc cctgggatat cggcttcgga gtgttcgccg
     5581 ccgcgagtca tcatcggggt gaccacaaca atctcctgca tgcctcgggc ggccgcctga
     5641 tccaacgcct ggtccaggct gggtgcgcag aactcgttga atcccacaat cacctcatgg
     5701 cccgtcgcct cccgcagcgc ctcggccagg gtatgcgagc ctgcccagaa tgggtcattc
     5761 cgctcagtgc gcggccaggt tcgcagcttt gcctccagct cggaatgccg gctctccagg
     5821 gcctggcggc gttcgccggt agccctttct aaggcggcgt gaaggcccat cagctcggcc
     5881 aattcctccg atgggaagtc attggcggga gcaccatgca tagccagtac aacggtgagg
     5941 ctcatcggtc gacactctcc aggtgatggc cacggtgatc ggagcgtccg gtcacgggac
     6001 cgtcccctcc cgatttgatt gcgtccgatt gtcctaccca tccattaggc ttgctacgcg
     6061 ccgaacggcg tacggcgccc gccggagaac ggcgctccta ggccctgcct ggcaggggta
     6121 gagtagccga aggggttatg ccactcgcgc cccctttggg agataaggat gaccatattg
     6181 gaaacccctg aggcgaccgc cgagatcacg ctggacgtgc gggggatgac ctgtgcttct
     6241 tgcgtggcgc acgtggaggg agcgctgacc gacctcgaag gcgtaacggc tgcagcggtg
     6301 aacttggggc tgggcacggc ccgggtcggc tatgtgcccg gtctggtggc gatcggccag
     6361 atgaagcgcg ccgtgcgcga agctggttat gaagcccagg aacgcagtca ggggctggat
     6421 gcgctggatc gagagcgagc agctcgggag ggggaaatcc ggcgccaagg ccgcaatttg
     6481 gccatcgccg gcgcggtggg catgctggtg atggtcggca ccttctacga catgcttggc
     6541 ccactgcgca gctggatccc cgcgtggctc tcctacaagt gggtgatcgg gctgctgacc
     6601 acccccattg tgttcggacc cggccgccag ttcttcacca actcctggcg cggcctcaaa
     6661 cacggcgtca ccgacatgaa tctcttgtac gccaccggga tcggcgcggc ctacgggatc
     6721 gcggtgatca acacgctgtt ccctaaggcc gggtttggcg gcgaaggtgc caccttcttc
     6781 gagagcgctg ccttgctcac cgccttcgtc gtgctgggcc gttggctgga ggccctcacc
     6841 cgcggacgaa cttcggaggc catccgcaag ctgatgaagc tgcagcccaa gcttgcccgc
     6901 gtgctgcgcg ggaccactga cgaggaaata ccggccagcg aggtggaggt cggcgacttg
     6961 ctgctggtcc ggccgggcga gagcctggcg gtggacgggc tggtggaaga gggctactcc
     7021 gcggtggacg aatcgatgct aaccggcgag agcctgccgg tcgagaagaa agccggcgac
     7081 tcggtcattg gaggcacgct gaacaagagc ggcgctttca ggttccgggc gacgcgggtg
     7141 ggaagacaga ctgccctggc gcagatcatc aagctcgtgg aagacgccca ggcgagcaaa
     7201 gctcccatcc agaagctggc cgactgggtg gccggccact tcatcctggg cgtgcacatg
     7261 cttgccgcgg tggtcttcgt cttttggttt ttctttgggt acgagcatta cttcgacccg
     7321 aacagctctt tcattctgtc accttatcgg ctgggcgaga tcggcgtctt tggtttctcg
     7381 ctgctgctct cggtcacggt gctggtcatc tcttgcccgt gcgcggtagg gctggcgact
     7441 cccagcgcca tgatggccgg cgcagggaag gctgccgagc atggcgtgtt gttcaagggc
     7501 gccgaagcga ttgagaacac cagcaagctg caaactctga tcttcgacaa gacgggcacc
     7561 ctcacccgag gcgagccctc ggtaaccgac gtgctcgccc gcagctcacc gcccggccaa
     7621 gccgagtcag cgtgggccct ccgaccggat gccattctgc gtttcgccgc cagcgccgag
     7681 ctgaatagtg agcacccgtt aggagaggcc attgtccgtg gcgcgcagac ccgcggcatc
     7741 gagatcctcg atccggagcg ctttgagtcc attcccggac acggggtaga ggcggaggcg
     7801 gaaggccgcg cagtgttgct tggcaaccgg cgcctgatgg ccgagcgggc gatcggcatc
     7861 tcagattggc ttggcgaggc ggagcggctg gaggcagagg gcaagactgt gatgttcctg
     7921 gcggtggacg gccacccggc cggtttgatc gcggtcgccg acacgcttaa ggaccactcc
     7981 cgcgaagcca tccaacaact gcaccgcctg ggcctggagg tcgccatgat caccggtgac
     8041 aacctgcgca ccgcgcaggc gatcgcccgc caggtcggga tcgaccgggt gctggccgaa
     8101 gtcctgcccc aagacaaggc cgaagaggtc aagaagctcc aggcgctggg taagcgagtg
     8161 gcgatggtgg gcgacggagt caacgacgcc ccggcgcttg cccaggccga tgtgggcatg
     8221 gccatcggct ccggtacgga tgtgtccaag gagaccggag acgtcatcct gatcaaagac
     8281 gacctgcgcg atgtggtcgt ggcgctggaa gcggccaagg ccaccatgcg caaggtgaag
     8341 cagaacctgt tctgggcctt cttctacaac acactgggca tcccggtcgg cgccgggctc
     8401 ttgtaccccg tgatccgctt ggtggtgagc cccgagctgg ctgcagccct gatggcgctg
     8461 agctcggtgt cggtgacatt gaataccctg ctgctcaagc gcttcaagcc ctccttgcgg
     8521 ggagggcaac cgccccaacc cgaggcgcag ggcgcggaac agccgcgctg ggcaggcgcg
     8581 cccgccggtg gcgactagcc aggaggaagg ccgatggaac gcgaatctgt ggaaagagaa
     8641 atcgcacgcc gcagcgcact catctacctg ccgatctcgc tcggcgccgc attgctcttc
     8701 gtgctggccg ccagctccgc cggggagtac ccgacggtcg ctcgggtcgg gggagccatt
     8761 tgggtgggac tgctgagcct gatcgtctca atgccgctcg ttacggcccg ggtgaagacc
     8821 cggaagcgcg gccaaccgga gagctgaaac aaagtgctga tcgcccaacg aaatctcatt
     8881 caggagaaga cccgcttcgc gctcagtgtc agcggagttg cgctggccat catgctcgtg
     8941 ctcatcctgg acggattcca agtgggcatg ttccggcaga tcacctcgta cttggatcac
     9001 agcgaaggca gtctcgtcgt cgcccaggag ggggtcgtta atctgatcgg agcgacctcg
     9061 ctcttgccgc tggggacccg cgcacaagtc gaagctgtcg aaggagtgca aggggtggcg
     9121 cccatcctct cccagttcgt catcctcgac ctgcacgaca agaagcagcc ggcgtacctg
     9181 gtggggtatt ct
//
LOCUS       DATDPQ010000334         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2292391, whole genome shotgun sequence.
ACCESSION   DATDPQ010000334 DATDPQ010000000
VERSION     DATDPQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292391"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..906)
                     /gene="fdhD"
                     /locus_tag="VI520_07555"
     CDS             complement(46..906)
                     /gene="fdhD"
                     /locus_tag="VI520_07555"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HLF37773.1"
                     /translation="MLPRPGSKTRSQLWRVSDGNWSQRDDLLATEEPMEIRLLAGGQR
                     KTLSVTMRTPGSDFELAAGLLYTEGIIPDRHAIARISYCREPDLTEASRYNIVHVELV
                     SNKLPELQTLERSTFTSSACGVCGKAGLESLERRDLRPLPPGPEIAQEVILALAGKLR
                     AQQGVFEATGGLHAAALFDLSGELIALREDVGRHNAVDKLIGWALLRGLLPWTQRIVL
                     VSGRAGFEIIQKLVVASAPLVCAISAPSNLAVDLARAFNVTLVGFLRGERFNVYHGRE
                     RIRASVSARQ"
     gene            complement(884..>2201)
                     /locus_tag="VI520_07560"
     CDS             complement(884..>2201)
                     /locus_tag="VI520_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HLF37774.1"
                     /translation="RTSNFQELADELEQQSWEELERASGATQDEMLAFARMIYEARSA
                     VFVWSMGVTQHEFGEDTVSAIVNLALTRGFIGREKCGLMPIRGHSGVQGGAEMGAYAT
                     AFPGGLAIDPRNAAWLSGLYGFAVPSSPGLTAPQMIDAAHDGRLDLLLAAGGNFLDVL
                     PEPDYVREALEAVPLRVHLDLVLNRQMLLEAGEAVILLPAATRYEGPGAVTQTSTERR
                     VILSPEIRGPRPGEARQEWQVFTELARRARPMLAGLLDFKDTAAIRAEIGRVIPEYAG
                     IERLEQPGDQFQYGGPQLCWGWQFPTPDGKAHFTPVAPPRADLPEGAFMVSTRRGKQF
                     NSMVHEARDQLTGASRETVLISPADAGRLGLCDGDRIRLSNPVGSFTGRAQIAPVRPG
                     NLQLHWPEGNVLIDRHRRSPKSGIPDYNAAVWLEKLEVRDAASPRE"
BASE COUNT          352 a          734 c          714 g          401 t
ORIGIN      
        1 ccgggtgctc ccaggggcga gctggccaga ttttctgcag gtggcctact ggcgagcgct
       61 cacgctcgcc cggatcctct cccgcccgtg atacacattg aaacgctcac cccgtaggaa
      121 gcccaccagg gttacgttga aggccctggc caggtcgacg gccagattgc tgggggccga
      181 gatggcgcac accagcggag cgctagccac gacgagtttc tgaataatct cgaagccggc
      241 tctgccactc accagaacga tgcgttgggt ccagggaagc aatccccgca atagggccca
      301 cccgatgagc ttgtccaccg cgttgtgtcg cccgacgtct tcccgcagcg cgatcagctc
      361 tccgctcagg tcgaagaggg ccgcggcatg cagaccgccc gtggcctcga agactccctg
      421 ctgggcccgc aacttgcccg ccagggccag gatgacttcc tgagcgatct ccggtccggg
      481 tggcagcggg cgcagatcgc gtcgctcgag cgattccagt ccggccttgc cgcacactcc
      541 gcaagcgctg ctggtgaagg tgctgcgctc caaggtctgc agctcgggaa gcttgttgct
      601 gaccagctcc acgtgcacga tgttgtagcg ggaggcctcg gtgaggtctg gctcgcgaca
      661 gtagctgatc cgggcgatcg catgccggtc ggggattatt ccttcggtgt agagcaagcc
      721 agccgccaat tcgaagtcac tgcctggagt gcgcatcgtg accgatagag tttttcgctg
      781 tccacccgcc agcaggcgga tctccatcgg ttcttcggtt gccagcaggt cgtctcgctg
      841 cgaccaattc ccatctgaca cccgccacag ttgggagcgg gtcttactcc cggggcgagg
      901 cagcatcgcg cacctccagt ttctcaagcc agaccgccgc gttgtagtct gggattccgg
      961 acttcggcga acggcggtgg cgatcgatta ggacattccc ctccggccag tgcagctgca
     1021 ggttgccagg ccgaaccggc gcgatctgcg cccggccggt gaagctgccg accgggttgc
     1081 tgagccggat ccgatcgccg tcacacagcc caagccggcc agcgtcggcc gggctgatga
     1141 gcaccgtttc ccggctcgcc ccagtgagct ggtcgcgggc ctcgtggacc atgctattga
     1201 actgcttgcc ccgccgggtg ctgaccatga aggcgccctc gggcaagtcc gccctcggcg
     1261 gcgcgaccgg ggtgaagtga gccttgccgt ccggggtagg gaactgccac ccccagcaga
     1321 gctgcggtcc gccgtactgg aattggtcgc cgggctgctc gagtcgctcg attccggcat
     1381 actccgggat cacccgcccg atctcggccc gaatggcggc ggtgtccttg aagtcgagta
     1441 ggccggccag catcgggcgg gcccgtcgcg ccagctcggt gaagacttgc cattcctggc
     1501 gtgcttcacc agggcgcggc ccgcggattt cggggctcaa gattacccgc cgctcggtgc
     1561 tggtttgggt caccgcaccg ggtccttcat agcgggtggc ggccggcagc agtatgaccg
     1621 cctcgccggc ctcgagcagc atctgccggt tcaatacgag atcgaggtgc actcgcagcg
     1681 gcacggcctc gagcgcctcc cgcacgtaat cgggctcggg cagcacgtcc aggaagtttc
     1741 ctccggctgc aagcaagaga tccagcctgc cgtcgtgggc ggcgtcaatc atttgtggcg
     1801 cggtcagccc tgggctggag ggaactgcga agccgtacag gccgctgagc cacgcggcgt
     1861 ttcgcgggtc tatggccagt ccacccggga aggcggtggc ataggctccc atctcggcgc
     1921 cgccctgcac tccggagtgg ccgcggatcg gcatcaggcc gcatttctcg cgaccgatga
     1981 acccgcgggt gagcgccaga ttcacgatcg cacttaccgt gtcttcgccg aattcatgct
     2041 gggtgacgcc catcgaccag acaaagactg cgcttcgtgc ctcatagatc atacgggcga
     2101 aggcgagcat ctcgtcctgg gtcgcgccgg aggcccgttc gagctcctcc caagattgtt
     2161 gctctagctc gtcggccaac tcctggaaat tgcttgtgcg t
//
LOCUS       DATDPQ010000335         4943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3887480, whole genome shotgun sequence.
ACCESSION   DATDPQ010000335 DATDPQ010000000
VERSION     DATDPQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887480"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VI520_07565"
     CDS             <1..918
                     /locus_tag="VI520_07565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLF37775.1"
                     /translation="WFGNQLVTLYLPLWLASVVCGVSAFGRTRRLMLVEAILLVLGMA
                     MLLMSRSRLSLFSFLLMLAALIGYGLWRVAGWLVKKAGGAERLSTSRLRRGLQLVLLM
                     VGVVALGAGWMAVARAVIERDPRTSQLLSTPAQLDSIRSEGPYETVYAVANRAAFAER
                     LVYWRAGFWPFEHYPVLGVGLGNAGFFFEDGLPAYGHRLVEIQNLLDPANPNFPNPKS
                     FWLRVLGETGLVGFSAFLVWLGVLAVGGIALMREAVGIDRVIGVAGLLAILAQVSEGF
                     SLDTFALPQLWIIPGLLTGAARRQRQPHG"
     gene            911..1966
                     /locus_tag="VI520_07570"
     CDS             911..1966
                     /locus_tag="VI520_07570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HLF37776.1"
                     /translation="MDNALSDLFPIVLLGCVAALGFSPLAARAAASLGLIDVPGSAPH
                     KAHPAATPLVGGPVLALAVAVSYFVVRPPVDRQIVGILFAGLLILVWGLLDDRFRVPP
                     LAKLAGQVLAAVVLIGYGVEVRITRIPGVDLGLTLLWVVGMINAFNFVDSMDGLALGL
                     ASVATGFFMLVTLDAGQPELAAFSAAILGAAIGLFFFNASPARLFLGDSGAQLLGFAL
                     AAIGIAYVPAGAGLPQGVSWFVPILVLGVPIFDSTLVVVSRLRRGRPLYRAQRDHTYH
                     RLVQLGVHPSRSVLAMHVAAVLLGLLAFVALDLTVFEANLLFGVSLLSGLIGILMLER
                     LFPASAPVNQSGAGTRS"
     gene            1967..3412
                     /locus_tag="VI520_07575"
     CDS             1967..3412
                     /locus_tag="VI520_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37777.1"
                     /translation="MDSPVPGSGSPTEEDAPPRSKRRRNNNIGYVVVLLGFILGRWQI
                     SNTDADHAFADTLGYARVSETGLGSRLFWGGERPFTVPLIYQALGLEAKDPPDQQAMR
                     RVTSFQIGLSVLSWISLATAVAWATQGRWSRLAAFGLILAFATSLDITQWDRILLSES
                     VSSSIFAEIVGGSILGVQLWAPETGGPDRLWQILFVAYMSLLVVFFAFARDSNGYIVL
                     AVSIGLAGSVCLPAVRTRPVAPIAGILALIMAGTFCFQDRSTNLGKRWLGPFLNVFSA
                     RILPVEEFVEFFSDRGFPAEAITEEMYLGRPVFLSQLQQTQEGGRLLEWIEKNGKSTY
                     LQFLASRPAQTLGKPIQRAGSMVNVDSSEYRLRERPDPAWAVLISDLMFPTRTEFLLG
                     FALLAPVVLLSTKNRLRTFQLTVTGLALMVVSYPLAVVIWHADTIELERHSYQLALQV
                     RLAAWLLVIGAMPSLSDLVARVTHTRLVTET"
     gene            3426..>4943
                     /locus_tag="VI520_07580"
     CDS             3426..>4943
                     /locus_tag="VI520_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37778.1"
                     /translation="MGLLAWLTSGLFVFLLSYSVHRLLRTDRQALEVSSWLFLLVFST
                     TLLMMGVGLVGWLQPAPILLLSSAGIAGTFTIGSLRRDLALIKGDSRFLVKVARLWWA
                     SLPFWLRMLTATAVLLSTARFLFLIWALPPFVWDSLSYHLSNVAHWVQVGRIETFETP
                     VVRIFNPANYEVLATWFAVFVHHDAVIEAAGIPAYLLAVLAVYAAARGLGRSRAASWM
                     GALAYGSTPAVILAATGTKNDIHIAGYYLLALAVVCDLSFRHEGTSLRKVLGRMTLFA
                     LVLLTALGTKTYILHLAPGLIVIGASARGSDGRRTDWSSRLKSGWQEFRALARQQRMI
                     LIALLGSALFLGSFWNLRNWVVSGNPFYPYDVLVGGRAVFEGPESEFRVSLGELAANL
                     KSFASKFGDWQDPIRPDLPNTTGWGWFAYTIGLPAMAWAAIRRPRSRPLQLGFGLSFI
                     LLMLSTGPSPWNMRFAIWFPALFSLCFAELADALPPILPRPVRIGLVGYFVFMLTL"
BASE COUNT          760 a         1471 c         1548 g         1164 t
ORIGIN      
        1 tggttcggca accaactggt gacgctctac ctcccgctct ggttggcgag cgtggtgtgc
       61 ggggtctcgg ctttcggccg aactcgaagg ttgatgctgg tcgaggcgat tttgttggtg
      121 cttgggatgg cgatgctcct aatgtcccgc tccaggctga gcctgttcag ctttctactt
      181 atgttggctg cgttgatcgg atacggactg tggcgagtgg cgggctggct ggtgaagaag
      241 gccggggggg cagaaaggct gtccacttct cgattgcggc gcgggctgca gctggttctc
      301 ttgatggtcg gtgtcgtcgc tctcggcgcc ggttggatgg ccgtggcgcg tgctgtgatc
      361 gagcgagacc cgcggacgag ccagctactg tcgacccccg cccaactcga ttcgatccgc
      421 agcgagggtc catatgagac cgtatatgcc gttgcgaacc gagcggcctt cgcggagcga
      481 ctggtctatt ggagagccgg tttctggccc ttcgaacact acccggtcct gggagttggc
      541 ctgggcaatg caggcttctt ctttgaggat ggacttccag cctatggaca tcggctggta
      601 gaaatccaga acctgctcga cccggcgaac ccgaacttcc ctaatccgaa gagcttctgg
      661 cttcgggtgt tgggggagac cgggctggtg ggtttctcgg cgtttctggt ctggctcggg
      721 gtgcttgcgg tgggggggat agcattgatg agggaggccg tcggaattga ccgggtcata
      781 ggcgtcgccg ggttgttggc gatcctggcc caagtgagcg agggatttag cctggacacg
      841 ttcgcattgc cccagctctg gatcatccca ggtctgctta ccggcgcggc ccggcggcag
      901 cgccaaccac atggataacg cgctctcgga tctcttcccg atcgtgctgc tcgggtgcgt
      961 cgcagcgttg ggcttctcgc cattggctgc ccgggcggcg gcaagccttg gcctgatcga
     1021 tgtgccgggt tctgcccctc ataaggctca ccccgccgcc accccgctgg ttggtggccc
     1081 ggtcttggcc ctcgcagttg ccgtctccta ttttgtggtc cgccctccgg tcgaccgaca
     1141 gatagtgggg atcctcttcg ccggcctgct gatcctcgtc tggggcctgc tggacgatcg
     1201 gtttcgagtt cccccgcttg ccaagctcgc cgggcaggtg ctcgcagcgg tcgtgctaat
     1261 cggatacggg gtcgaggttc ggatcactcg gattccgggg gttgaccttg ggttgaccct
     1321 gctctgggtc gtcggaatga tcaacgcatt caacttcgtg gacagcatgg acgggctcgc
     1381 ccttggcctg gccagtgtcg ccaccggttt cttcatgctg gtgacgctag atgccggcca
     1441 gcccgagctg gccgcgtttt ctgcggcgat cctgggcgcg gcgatcggcc tgttcttctt
     1501 caatgcctcg ccggccaggt tgttcttggg agactcggga gctcagcttc tcggcttcgc
     1561 gctggcggcg atcgggatcg cgtatgttcc ggccggggcg gggctccccc aaggggtctc
     1621 ctggttcgtc ccgatcctgg tgctcggggt gccgatcttc gactcgaccc tggtcgtggt
     1681 gtccaggttg aggcgcggcc gaccgctgta ccgggcccag cgcgaccata cctatcatcg
     1741 gctcgtgcag ctcggggtcc acccctcgcg atcggtgttg gcaatgcatg tggccgcggt
     1801 gctgcttggt ctgctggcgt tcgttgcgct tgatctcacg gtcttcgagg caaacttgct
     1861 cttcggtgtc agcctgctgt ccggactgat tgggattctc atgttggagc gattgttccc
     1921 ggcgagtgcc ccggttaatc aatccggtgc cggtacacgg tcctaggtgg actccccagt
     1981 gccgggctct gggagtccaa ccgaagagga tgccccgcca agatccaagc ggcgacgaaa
     2041 caacaatatt gggtatgtcg tagtccttct ggggttcatc ctgggaaggt ggcaaatatc
     2101 caataccgac gccgaccatg ccttcgccga caccttgggg tacgcccgag tctcagagac
     2161 tggtttgggg agtcggctgt tctggggggg agagcgtcca ttcaccgtgc cgttgatcta
     2221 tcaagccttg ggcctggagg cgaaggatcc tcccgatcag caggcaatga gaagggtcac
     2281 ctcatttcag atcggcctct cggtcctctc ctggatttca ctagcaaccg cagtcgcctg
     2341 ggccactcag ggccgatgga gtcggctggc ggcatttggc cttatcctgg cattcgccac
     2401 cagtttggat atcactcagt gggatcgaat actgctttcc gagtcggtct cctcctcgat
     2461 cttcgcagaa atcgttggcg gttcgatact gggtgttcag ctatgggcac ccgaaacagg
     2521 tgggcccgat cggctgtggc agatcctctt tgtggcctac atgtcattgc tggtcgtgtt
     2581 cttcgcgttc gcccgcgata gcaacggcta cattgtgctt gcagtgtcca tcgggttagc
     2641 gggtagcgta tgtctgcctg ctgttcgtac ccgacctgtc gctcccatcg cggggatcct
     2701 tgcattgata atggcaggga ccttctgctt ccaggaccgt tctacaaacc taggaaagcg
     2761 ctggctgggg ccattcctca atgtgttcag tgcccgaatc ttgccagttg aagaattcgt
     2821 cgagttcttc tcggatcggg gtttccccgc tgaagccatc accgaagaga tgtacttggg
     2881 gcgcccggtg ttcctgagcc agctgcagca aacccaggag ggcggccgac tgctcgagtg
     2941 gattgagaag aacggcaagt caacctacct tcagtttctg gcctctcgcc cggcccagac
     3001 ccttggaaaa ccgatccaaa gagccgggtc aatggtcaac gtcgactcct cagagtacag
     3061 attgcgagaa cggcccgatc cggcctgggc agttctgatc agcgatttga tgtttccgac
     3121 ccggacggag ttcttgcttg gcttcgcgct gcttgcaccg gtcgtcctgc tctcaacgaa
     3181 gaaccgactc cggactttcc agctcacggt gaccgggctg gcgctgatgg tagtgtccta
     3241 tccgctagcc gtcgtgatct ggcatgccga taccatcgag ctcgagcgtc acagctatca
     3301 gctagctctc caggtcaggc tggccgcctg gctactcgta atcggcgcaa tgccgtcctt
     3361 atcggacctc gtggccaggg taacccacac acgcctggtc acggagacgt agaggcaaag
     3421 tgatagtggg gttgctcgcc tggctgactt ccggcctctt cgtcttcctc ctgagttact
     3481 ccgttcaccg cctgctgcga accgaccgcc aggcgttaga ggtcagctcc tggttgttcc
     3541 tgctggtttt cagcactacc ttactcatga tgggggttgg cctggtcggg tggcttcaac
     3601 cggcgccaat actgttgctc agcagcgctg gcatcgcggg aaccttcaca attgggtcgc
     3661 tcaggagaga cctcgcactg ataaagggag actcgaggtt tctggtcaaa gtcgccagac
     3721 tctggtgggc gagtttgccc ttttggttgc gaatgctcac cgcaacagcc gtgctgctca
     3781 gcaccgctcg cttcctattc ttgatctggg cccttccccc gttcgtatgg gattcgttga
     3841 gctatcacct gtccaacgtt gcccattggg ttcaggtcgg ccggatcgag acttttgaaa
     3901 ccccggttgt taggatcttc aacccggcca actatgaggt gctggcaacc tggttcgcag
     3961 ttttcgtcca ccatgatgcg gtgatcgaag cggcgggcat cccggcctac ctgctcgcag
     4021 tgcttgcggt ctatgccgct gcccgagggc tgggccgcag tcgtgcggcg tcatggatgg
     4081 gcgcgctggc ttatgggtcc actcccgcag tcatcctcgc cgccaccgga accaagaacg
     4141 acatccacat agccggctac tacttgcttg cgctggcggt ggtctgcgat ctgtccttcc
     4201 gccatgaggg tacttctctt aggaaagtcc ttggtcggat gacgctgttt gcgctagttc
     4261 tcctgaccgc cctcggtacc aagacctata tcctacatct ggcccccggg ttgatcgtga
     4321 tcggcgcatc ggcaaggggg agcgacggca ggcggaccga ctggtccagc cgcctgaaat
     4381 ccggctggca ggagttccgg gccctcgccc gacaacaacg aatgatcctg attgcgctgc
     4441 tcggctccgc attgttcctg ggcagcttct ggaatctgag gaactgggtt gtctcgggga
     4501 atccgtttta tccctatgat gttttggtgg gaggcagagc cgtgttcgag gggcccgagt
     4561 ccgagtttcg ggtcagcctg ggcgagctgg cggccaacct caagagcttt gccagtaagt
     4621 tcggagattg gcaagatcca attcgaccag acctgcccaa cacgaccggc tggggctggt
     4681 ttgcctacac gattgggctc ccagcgatgg cctgggcggc gattcgccgg ccgcgcagcc
     4741 gacccctgca gcttggcttt gggctttcct tcatcctgct tatgctctcg accggaccca
     4801 gcccctggaa catgcgcttc gcgatctggt ttccagcgct cttcagcctc tgctttgccg
     4861 agttggccga cgctctgccg cccattctgc cgcggccggt acggatcgga ctggtgggat
     4921 atttcgtgtt catgctgacc ctg
//
LOCUS       DATDPQ010000336         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_974758, whole genome shotgun sequence.
ACCESSION   DATDPQ010000336 DATDPQ010000000
VERSION     DATDPQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974758"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VI520_07585"
     CDS             complement(<1..1032)
                     /locus_tag="VI520_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLF37779.1"
                     /translation="MVQAADLEAIGRLSPVWFRYTDLLVEHGEGAYLYDRDGKAYLDF
                     TCGIGVTNTGHCHPRVVKAVQEQAAKLLHGQVNIVHHAPLLELVDELGSIVPAALDGF
                     FFSNSGAEAIEGALKLARAATGRPNVVVFQGSFHGRTVGTMSLTTSKTIYRAGYQPLM
                     PGVFVAPFPYAYRYGWEPGQTRDWCLKELEYLLQTQTSPQETAAVLVEPVLGEGGYVV
                     PPDGFLAGLRAICNAHGMLLILDEIQSGFGRTGKWFALEHFQVEPDIVVVAKGLASGL
                     PLSAVVSRMELMKKWNPGTHGGTYGGNPLACAAAVATIRAIRDERMLENATTRGQQLM
                     AGLGQLREEH"
     gene            1318..>3161
                     /locus_tag="VI520_07590"
     CDS             1318..>3161
                     /locus_tag="VI520_07590"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HLF37780.1"
                     /translation="MFETTLNSMASFVRRLWAGRRPPPIIVGRAVALGAAYFVSGKLS
                     VLLAIPPGIASPVWVPSAIALAAVLRWGYGVGAGIWVGSFLVNIGILQGGGGLAAVAV
                     VSGIATGSTLQAIVGAFLIRRFVGIRGVFDSVSGTFRFIGAVLLACLLAPLFGAGSLY
                     LGGFIPPEVFRVTWLTWWLGDLVGILVVSPIFLVPWRLDRESVGEAAGLFSVLVAFAV
                     LVFEIPVLPPELIRPPVAYVFILLVIWAAFRFGQSGVVTTSIAISVFAIHGTIQGKGG
                     FAVGTTNESLLLLQSFIGLSTLTGLLLAAALTERGQAEEEVRRGHDELERRVRGRTAE
                     LTLANAALQDEIAERRSAEGALHESESILRGLFEFSPDAIVVTDQAGRIVRINAQTEM
                     QFGYGRQELLGQPVEVLVPDRFAARHSQHRKSYLVRPQARTMGGGLELYGRRRDGTEF
                     PADIMLSPVETSEGRTVIAVVRDITERKRSDDALRRSRDGLAELYQRLQELDLLKSKF
                     IGDVSHELRTPIANLGLYLDLIETGQPKKRKQYQIALREQIGRLTDLIEEISNLSNLE
                     QDQLTAAFGSVDLNAVVDLVVNEFQSRAKTAGLDLTFTPGGDPLLVRAV"
BASE COUNT          566 a          956 c          988 g          651 t
ORIGIN      
        1 gtgctcttcg cgcagctggc ccagaccggc catcagctgc tgtccgcggg ttgtagcgtt
       61 ctccagcatc cgttcgtccc gaatcgcccg aatcgtcgcc actgccgcgg cacaggccag
      121 cgggttgccg ccgtaggtcc ctccgtgggt tccaggattc cacttcttca tcaattccat
      181 tcgggagacc accgcagaca gaggcagacc cgaggccaat cccttggcca caaccacaat
      241 gtcgggctcc acctggaagt gctcgagggc gaaccacttc ccggtccgcc cgaagccgga
      301 ttggatctcg tccaggatca gcagcatgcc atgcgcgttg cagatcgccc gcaggcccgc
      361 taagaatccg tcgggcggaa cgacgtagcc gccctcacct aacaccggct cgaccagcac
      421 cgcggcggtc tcctgcggcg aggtctgggt ctgcagcaga tactccagct ccttcagaca
      481 ccaatcccgg gtctgccccg gctcccagcc gtagcggtac gcatacggga acggagccac
      541 gaagactccg ggcatcagcg gctggtagcc ggcgcggtag atggtcttgc tggtggtcag
      601 agacatcgtg cccaccgtgc gcccatggaa ggatccctgg aacacaacaa cgtttggccg
      661 accggtggcc gcccgcgcca gcttgagtgc gccctcgatg gcttccgcgc cgctgttgct
      721 gaagaaaaag ccgtccaggg ctgccggcac gatgctgccc agctcgtcca ccaattccag
      781 cagcggtgcg tggtgcacaa tgttgacctg accatgcagg agtttggcgg cttgctcttg
      841 gaccgctttc acaactcggg gatggcagtg tccggtgttc gtgacgccaa ttccgcaggt
      901 gaagtcgagg taggccttcc catcccgatc gtacaagtag gcgccttccc catgttcgac
      961 gaggaggtct gtgtaacgga accaaacggg ggaaagccgc ccgattgctt ctaagtctgc
     1021 agcctggacc atggttactt tcctcggccg agctgaaatc tcatgcaatt cgagttcatt
     1081 atcatccagc tactttccgt ggtcgaggac gagatccaaa ttctggcgag aggccagcct
     1141 gatcggtgct ggcccgatcg ggccagcagc ccactgccgg gcagcccgcc tcattttgcg
     1201 ttcaacccgc ggttgtatac tcggggcgtc ctccggccga ctaccgcctc gccgcatgtt
     1261 gcggccggcc ttgatccgcc gcacaatccg cttcggccgg aaacggacac tgatactgtg
     1321 tttgagacaa cgctgaattc aatggcctcc ttcgtacgtc gcctttgggc cggacgaagg
     1381 ccaccaccca tcattgtcgg cagggccgtc gcgctgggcg ccgcttactt tgtcagcggc
     1441 aagctttcgg ttctgctggc catcccgcca ggcattgcgt caccggtctg ggtcccgtcc
     1501 gcgatcgcgc tggccgccgt cctgcgatgg ggatacggag ttggggccgg catttgggtc
     1561 ggttcgttct tggttaacat cgggattcta cagggcggcg gcgggttggc agcggtggcg
     1621 gtcgtcagcg ggattgccac cggctctacg ctgcaagcca ttgtcggtgc gttcttgatc
     1681 cgccgcttcg ttggaatccg cggagtgttt gacagtgtgt cgggcacttt ccgcttcatc
     1741 ggtgcagtgt tgctggcctg tttgcttgcg cccctcttcg gcgcgggcag tctttactta
     1801 gggggattca ttccacccga ggtctttcga gtgacttggc taacctggtg gttgggcgat
     1861 ttggtcggga ttctggtggt gtcaccgatc ttcctggttc cgtggcggct agatcgcgaa
     1921 agcgtgggtg aagcggcagg gctcttctcg gtcctggttg cgttcgctgt gttggtcttt
     1981 gaaatccccg tattgccccc cgagctgatt cgtccaccgg tagcctacgt tttcatcctt
     2041 ttggtgattt gggcggcctt ccggttcggg cagagcgggg ttgttaccac ttcgatagcg
     2101 atcagcgtct tcgccattca cggaacgatc cagggaaaag gcggatttgc agtagggacg
     2161 accaatgagt ctctactgct gctgcaatcc ttcatcggcc tgagcacgct gaccgggctg
     2221 cttttggcgg ctgcgctcac cgaacgcggg caggctgagg aggaagttcg cagaggccat
     2281 gacgaattag agaggcgggt ccgaggacgg actgcggaac tcacgctggc caacgccgcg
     2341 ttgcaagacg aaatcgccga gcgtaggagc gccgaggggg cgctgcatga gagcgagagc
     2401 atcctgcgcg ggctctttga attctcaccg gatgcgattg tcgttaccga ccaggcgggg
     2461 cgcatagtcc ggatcaacgc ccaaaccgag atgcagtttg gatatggccg gcaagaactg
     2521 ctcggccagc cagtcgaggt cttggtgccg gatcgattcg cggcgcggca ctcgcagcat
     2581 cgcaagagct atctcgtcag gccgcaagcc cgcacaatgg ggggcggctt ggagctttac
     2641 ggcaggcgtc gggacgggac cgaattcccg gcggacatca tgctgagtcc ggtggaaaca
     2701 agtgagggga ggacggtgat cgcggtcgtg cgggatatca ccgagcggaa acgcagcgac
     2761 gatgcgttgc gccgcagtcg ggacggcctt gcggagttgt atcaacggct tcaagaactc
     2821 gacctcctga aatccaagtt catcggtgat gtctcccacg agcttcgtac accaatcgcg
     2881 aacctgggac tgtacctgga tctgattgag accggtcagc ccaagaagcg aaagcaatac
     2941 cagatcgccc tcagagaaca aatcggacga ttgaccgatc tcatcgagga gatttcgaat
     3001 ctctccaacc tggaacaaga ccagctgacg gcagctttcg ggagtgtgga cctgaacgcg
     3061 gttgtcgacc tggttgtcaa cgaatttcaa tcgcgggcca agacggccgg gctggacctg
     3121 actttcaccc ccggcggcga cccattgctc gtacgagccg t
//
LOCUS       DATDPQ010000337         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1508569, whole genome shotgun sequence.
ACCESSION   DATDPQ010000337 DATDPQ010000000
VERSION     DATDPQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508569"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VI520_07595"
     CDS             <1..373
                     /locus_tag="VI520_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF37781.1"
                     /translation="KGDLIYASTKVAVTRWARRHAIQSDWAGAGILLNIVAPGLIQTP
                     MTQGMLDNPKTMDSYKELLPMPIGRYGQPEEVAELIAFLVSPENSLMVGQVIYIDSGS
                     EATTRGELGWVSAPQPATAGA"
     gene            complement(481..2109)
                     /locus_tag="VI520_07600"
     CDS             complement(481..2109)
                     /locus_tag="VI520_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37782.1"
                     /translation="MSAIQAGRAAWFVPIGLAVAVIAAGGCGGGRSPEGTPGTRTFTP
                     PTATATIDPNSVTVSASRSVATLAIATETPVSTIPAALDPEGPWLLGIGGEDRLVALN
                     PDGSGLTHLAHEPLWDSYPEVREGVELSGTGWIAVRTSANGWPVPPTDMSLDLFQLPD
                     VQPVRRIPLFSQELVARMGQIGEHRLPYEAEPVEGPNWRYEMVYLVVLAERNPPRWSP
                     DGRYLAFAAAMDGPSSDLYVYDTQTDQVRRLTDGPNQAAIMGWSPDSRWIIHVEASTY
                     MIADGGEIGGFPGDAVWAAAVDGSELKRLYEPARVEWILGWVADTVFLVEGWSGYIFL
                     HDLRSVDVATGAEQMHYSGKYYNSALASEAAVAVVDAVSDWRSEEEYLPGGLHLVPLD
                     GGEPKQLRTGVQEFNARWLQWFPQLDRFFAGGFGSPYLFTSSGEITHTFEDERGWPAV
                     SPDGSWLIFDEERERPGLRLYRSDGQLDRVISEENAFNFVWLPDSSGLYYLVQAGDSN
                     RLMVVSIPEAETVVLHPNPGLYRFVLVGRPKESG"
     gene            complement(2123..>2312)
                     /locus_tag="VI520_07605"
     CDS             complement(2123..>2312)
                     /locus_tag="VI520_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37783.1"
                     /translation="PVPTLLGLLTIPMAWRAFQGLRRHHAYPYRLIPANATTVFAHLF
                     TGLLMFVGYVVAVLIPAA"
BASE COUNT          480 a          738 c          708 g          386 t
ORIGIN      
        1 caagggtgac ctgatctatg cctcgaccaa ggtcgcggtc acccgctggg cgcggcggca
       61 tgccattcag tccgactggg cgggcgcagg catcctgctg aacatcgttg ccccgggcct
      121 gatccagacc ccgatgaccc aaggaatgct cgataacccg aagacaatgg acagctataa
      181 ggagttgctg ccgatgccga tcggccggta tggtcagccc gaggaagtgg ccgagctgat
      241 tgccttcctg gtcagccccg agaactcgct gatggtcggg caggtgatct acatcgacag
      301 cggaagcgag gcgacgaccc gcggtgagtt gggctgggtt tccgcaccgc aaccggcgac
      361 tgcgggcgcc tgacaaagcg gtcctgcgag ctgaatgagg gagagcctga ccgggatctc
      421 agtgagcccg caatgggagg gccgaactgg cgggccggtc tcccttactg ccgggctttc
      481 ctatcctgac tccttgggtc taccgacaag tacgaagcgg tacaacccag gattcggatg
      541 gagaacgacc gtttcggctt cagggatgga gacaaccatc agcctgttcg agtcgcccgc
      601 ttggaccagg tagtacaaac ccgacgaatc cggcagccag acgaagttga acgcattctc
      661 ttccgagatc accctgtcca actgcccgtc cgaccgataa agcctcaagc ctggtcgttc
      721 gcgttcctca tcgaagatca accaggaacc atcgggggat accgccggcc agccacgctc
      781 atcctcgaag gtgtgagtga tctctccgga tgaggtgaag aggtaaggag aaccaaaccc
      841 acccgcaaag aaacgatcca actgtgggaa ccattggagc cagcgcgcgt tgaactcttg
      901 gactccggta cgaagttgct ttggctcacc cccatccagt gggacgaggt gaagacctcc
      961 aggcaagtac tcctcttcgc tacgccaatc cgatacagca tcgacgacgg cgaccgcggc
     1021 ctctgaggcg agggcgctgt tgtagtactt gccagagtag tgcatctgct cggcacccgt
     1081 ggccacatcc accgatcgaa gatcgtgcag gaagatgtag ccgctccatc cctccaccag
     1141 gaataccgtg tccgcgaccc acccgaggat ccactcgacg cgggccggtt catacaggcg
     1201 cttgagttca ctcccgtcca cagccgctgc ccacactgcg tcgccgggaa aaccgccgat
     1261 ctcaccgccg tcagcgatca tgtaggtgct ggcctcaacg tggataatcc accggctgtc
     1321 tggcgaccag cccatgatcg ccgcctggtt gggcccgtcg gtcaggcgcc gcacctgatc
     1381 ggtctgagtg tcgtagacgt agagatcgga cgaggggccg tccatcgccg cggcaaacgc
     1441 caagtagcgc ccatccggag accaccgtgg aggattgcgc tctgcgagga cgaccaggta
     1501 gaccatttcg taccgccaat tgggtccctc gaccggctcc gcttcgtacg gcaggcggtg
     1561 ctctccgatc tgacccatgc gagcaacaag ctcttgcgag aacagcggaa tcctcctgac
     1621 cggctgaaca tccgggagct gaaaaagatc gagggacata tccgtgggcg gaaccggcca
     1681 cccgttggct gaggtccgca ccgcgatcca gcctgtcccc gacagttcga ccccttcccg
     1741 aacttcgggg taagagtccc acaggggttc atgcgccaga tgtgtcagcc cgctgccgtc
     1801 ggggttcaag gcgaccagcc gatcctcacc accgattccc agcagccagg gcccttcagg
     1861 gtcgagggcg gcgggaatgg tcgaaacagg agtttcggtc gcgatcgcga gtgtcgctac
     1921 cgaccggctc gccgagaccg tcacggagtt gggatcaatc gtagctgtgg ccgtcggagg
     1981 agtgaaagtg cgcgtacccg gagttccctc cggcgaccgt cccccgccac atcccccggc
     2041 cgcgatgaca gcgacggcca ggccgatggg gacaaaccag gcagctctgc ccgcttgaat
     2101 cgcgctcatt cagccgcctg atctacgccg ccggaatcaa gaccgccacg acatagccaa
     2161 cgaacatcag gagaccggtg aacaggtgcg caaagacggt cgtggcatta gccggaatca
     2221 gccggtaggg ataggcatgg tgacgccgca gtccttggaa ggctcgccag gccatcggga
     2281 tcgtgagcag tccaagcagg gtcggaaccg gc
//
LOCUS       DATDPQ010000338         8209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2544914, whole genome shotgun sequence.
ACCESSION   DATDPQ010000338 DATDPQ010000000
VERSION     DATDPQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544914"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VI520_07610"
     CDS             <1..666
                     /locus_tag="VI520_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLF37784.1"
                     /translation="VRRSNRQLHALSQRLFQAQEQERLHLSRELHDESGQLLAALMVQ
                     LGLLERDATQPEKVVQRLGNLKETTQGIQENLHKLAVDLRPASLDHLGLVTALDQYVQ
                     DFREQYGIKVEFEAVGMAKERLPTEVETTIFRIVQESLTNVILHAEATRVDIILSQRG
                     GAVAATIEDNGVGFNPSASTFEDHLGLFGMRERVEMLGGSLTVESSPGKGTTVNVEVP
                     GDA"
     gene            659..1297
                     /locus_tag="VI520_07615"
     CDS             659..1297
                     /locus_tag="VI520_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37785.1"
                     /translation="MPRILITDDHAIVRTGLRTLLTAEPGMELVGEASGGFEAIELAE
                     KVRPEVLLLDLSMPDLDGIQVTRALKAKLPDLRILILTVHEDQALLREAIRAGASGYI
                     VKRAAESELISAINVVMRGDMYVDPSLMRALLVDSVPPASQSNNAPEQLTPREVQVLK
                     LIVQGYTNRQVGAELNISVRTVEGHRANLAGKLGLQSRVDLVRYARERGLLG"
     gene            1536..3053
                     /locus_tag="VI520_07620"
     CDS             1536..3053
                     /locus_tag="VI520_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37786.1"
                     /translation="MIQMRGINKSFADVIANENVDLDIYPSEIHALLGENGAGKSTLV
                     KILYGFYRADSGRIELDGRPASIKTPREARAAQIGMVFQDFTLIPAFSVAENIALFLP
                     DLDPVVDLDDVCLRIGTLSERYGLQVDPRQMVGSLSIGEMQKVEILKLLLSNARLLIL
                     DEPTRVLAPHELASLLNVLRNLRDDGYAIVLITHKLKEVLECADRITVLRRGRVAGSL
                     LRREVNEQRLIDLMFEMRLSAPQVNGSPPARDRSPVLELRGISTRAQGDEMTLRNLEL
                     KIYPGEIVGIAGVSGNGQKELGDLILGAKRSQAGTKLLFGEDVTRKSVRGLRSRGVSF
                     IPENPLAMASVPFMTVLENMALTRTQQYARWGGMGIGWRAVRDDLAQASAELGFSLPI
                     FAPAKSLSGGNLQRMVIVREMAQRPKLIIASYLTRGLDLQSTYAARHALIQARVEGCG
                     VLLISEDLEELFTLSDRLIVLHGGRIVGTFRPQDTTISKVGHLMTGSEGQPSDNV"
     gene            3118..4095
                     /locus_tag="VI520_07625"
     CDS             3118..4095
                     /locus_tag="VI520_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37787.1"
                     /translation="MIVFAFLLFGIILLIAGNDPIQSYRDLFGSTLGSAYGLSEVFVA
                     MTPMLLTALAVALPSRIGLINVGVEGQLYLGAGLATWGALAFGDLPAWLLLPWMILLG
                     MIGGSLWALIPGILRAMGLVNETITTLLLNSVAPLIVSFLVFGFWHSPVDTNKTPNFP
                     PAARLPTLFDTRIDLSLVIGLLLLAVFWLAMKYSRWGLEMRAIGGNPQAALRNGIPLK
                     TYLISVMCLGGAIGGLAGMAQVSGFYGVLLANFSRGIGYMGFLISWLAGGNPLGIVLT
                     SFGVAIITSGGSLLQITRDVPYAVINILLAFTLFVVLARPQLAGRRKAA"
     gene            4092..5009
                     /locus_tag="VI520_07630"
     CDS             4092..5009
                     /locus_tag="VI520_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37788.1"
                     /translation="MTLLAVLGVLSSAIYSGTSLLYTTLGEIIDQRSGIVNLGLEGVL
                     LVSASAGFALTAITGNPYLGVAVSMLIGGVMNLSLGYLTVTRRANQLASGLSLMFFGF
                     GLSALIGRPYVGARIVGLPRLPLPGLSNLPVLYSSLFRFDLLIYLAIPAAVLVWWLLF
                     RTRWGLGLRAVGESADIAFASGRDPRLIRYQALFVGGLFAGLGAAHLSIAYAMNWTEY
                     MTAGRGFIAVALVIFSKWHPLRAIAGALLFSAAVAFQLLLQSSGTQISPFLLSTIPYL
                     VTILVLMLWGGTRRHSAPASLGRVYLGTE"
     gene            5114..6283
                     /locus_tag="VI520_07635"
     CDS             5114..6283
                     /locus_tag="VI520_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLF37789.1"
                     /translation="MRVKTFYSLLAGLLMTALVLSACAQAAPTTEAPAVPPEEPAAEP
                     TLEQPVVPAAEEPCLIIGALYGGPITDAGYNQAMYESVMTIKENISCVEIIEAENVPE
                     EAGATSTMENMVQQGAKLIIATAFNHQYPALELSAKYPDVIFEHAGGWEMGPNFANFF
                     GEPPDTWYMMGVAAGLMTESNQLGFVAAFPLGWTLTFINAFELGAQSVNPDVETTVTY
                     TFSWGDAAKEADATNSLINQGVDVITMHVDSPATVLSTAESRAVYSIGFQSLAAQQFA
                     PEYWISGIGYTLGDKLTFLASTVLDGSWEPIFLRCGIADGCMAMAPFGPKVSAEVQDA
                     VNQARADIEAGGIVVFAGPVVDQEGNVKIPEGEVLSPDVMGSVDWFVQGVIGSPK"
     gene            6369..7616
                     /locus_tag="VI520_07640"
     CDS             6369..7616
                     /locus_tag="VI520_07640"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HLF37790.1"
                     /translation="MEIKQKIEKANAEAVQRMTEGEPVLVDIAPAGEVIPGLKDRMIT
                     HAGPPIEWERMCGAQQGAIIGMTLYEGWAANPDDARAMLERGEIRLEPNHHHGSVGPM
                     AGTISPSAPVWVVENRAHGNRGFCRQVEGRQQFGEFSDGALGGLRLWRDLWAPTLREA
                     LQAIGGLELKPIITQALQMGDELHNRHTASSSLFANRMAVAMAEAGIPKDRALPTLKY
                     VTGHNLLFLGLAMASGKVIADPAMAIDCSTIVTAMARNGTEFGIRVSGLGEDWFTAPA
                     PSVEGLFLPGYSAKDAGLDMGDSAITETVGWGAFTLAGAPGILTLVGGTSEEALAYTR
                     EMRKITTALHPTYRMPALGFAGISVGIDIRKVVQSNLAPIIDTAIAHKDAGYPIIGAG
                     LVRAPMECFKQALIAFGKRYSEA"
     gene            7647..>8209
                     /locus_tag="VI520_07645"
     CDS             7647..>8209
                     /locus_tag="VI520_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37791.1"
                     /translation="MASGFIVRKNRYYDSVFLMGVDRRLSQLPGVRQTAVLMASENNK
                     RFLAEMGIRGAELDAAQADDLIVAVIADSPKLVEQVLGNLLDEALMSLETSTRVSDLR
                     TLEEGLRAAPTANLAVLTIPGEYVDREAQAALESGLNLFIFSSNVPIEAERKLKQFAS
                     ERKLLVMGPDCGTSILNGVGVGFANVVR"
BASE COUNT         1565 a         2480 c         2509 g         1655 t
ORIGIN      
        1 gttcggcgca gcaaccggca gctacatgcc ctttcccaac gcctgttcca ggctcaggag
       61 caggaacgtc tgcatctgtc acgtgagctg cacgatgaat cgggtcagct gctcgccgcg
      121 ctcatggtgc agctcgggct cctggagcgg gatgccaccc agccggagaa ggttgtgcag
      181 aggctgggga atttgaagga aaccacccag ggaatccagg agaatctgca caagctggcg
      241 gtcgacctgc ggccggccag cctagaccac ctcggcctgg ttaccgcgct ggatcagtac
      301 gtccaggatt ttcgggagca atatggaatc aaggtcgaat tcgaagcagt aggcatggcc
      361 aaggagcgcc taccaaccga ggtcgagacc acgatcttcc gaatcgttca ggagtcattg
      421 accaacgtca ttctgcatgc cgaggcgacc cgggtggaca tcatccttag ccagcgcggc
      481 ggggcggtgg cggccacaat cgaggacaac ggcgtggggt tcaaccctag cgcctccacc
      541 tttgaagatc acctcggcct gttcggaatg cgtgagaggg tcgagatgct cgggggcagc
      601 ctgactgtcg agagctctcc cggcaaagga acgacggtca acgtcgaagt gcccggcgat
      661 gcctaggatt ctgatcaccg acgatcatgc catcgtgcgc acgggactgc ggacgctgct
      721 cacagccgaa cccgggatgg agttggtcgg cgaggcgagc ggcggattcg aagcgatcga
      781 gctggcggag aaggtgcgac cggaggtcct gctgctcgat ttgagcatgc ccgacctgga
      841 tgggatccag gtgacgaggg cccttaaagc caaactccca gatctgcgca tcctgatcct
      901 caccgtacac gaagatcagg ccctgctgcg ggaggcgatt cgcgccgggg catcggggta
      961 catcgtgaag cgggccgccg aaagcgagct aatctccgcc attaacgtcg tcatgcgcgg
     1021 cgatatgtat gtggatccgt ctctcatgcg cgccctcctg gtcgacagcg tgccgcccgc
     1081 cagccagtcg aacaacgctc ccgagcagct caccccgcgt gaagtgcagg ttttgaaatt
     1141 gattgtccag ggctacacca accgccaggt cggagcagag ctcaacatca gcgtgcggac
     1201 cgttgagggc catcgggcca atctcgccgg caaactcggc ttgcaaagcc gggtcgatct
     1261 cgttcgctac gcccgtgagc gcgggctgct cggctgaacc ggatcgagtc gcggaccgcg
     1321 tagaaactac ctgtgcgagg accgtagata gcaacggacc tccactttgt gaaaatcccc
     1381 actttctacg cttaccctcc ctagcccagc cctctaagct agatctgacc ctaggtctgc
     1441 ccaacttttc gcccgaatga ggcgagaggc agtccgcgcc aacgccggat gagcgcatgc
     1501 cagagccgtc catcaccctc tccctaaggc ctccgatgat ccagatgcga gggatcaaca
     1561 agtcctttgc ggacgtgatc gccaacgaaa acgtcgatct cgacatttat ccatcagaaa
     1621 tccacgccct gctcggtgaa aacggggctg gaaaaagcac cctggtaaag atcctctacg
     1681 gtttctaccg ggcagattcc ggccgcatcg agctggacgg ccggccggcc tcgatcaaga
     1741 ctccccgcga ggcacgcgcc gcccagatcg gcatggtctt tcaggatttc accctcatcc
     1801 cggctttcag tgtggccgag aatatcgcac tgttccttcc agatctcgac ccggtcgttg
     1861 atctggacga tgtctgcctc cgtatcggga ccctttccga gcgatatggt ctgcaggtcg
     1921 acccgcgcca gatggtcggg tcgctgtcca tcggtgagat gcagaaagtt gagatcctga
     1981 agctgctgtt gtcaaacgcg cgcctgctca tcctggacga gcccacccgg gtcctcgccc
     2041 cgcatgaatt agcctcgctg ctgaacgtgc tgcgcaatct gcgtgacgac ggctacgcga
     2101 ttgtgctcat cacccacaaa ctcaaagaag tgcttgagtg cgcggaccga atcaccgttc
     2161 tacgtcgcgg ccgggtggca ggatccctgt tgcgacgaga ggtcaatgag cagcgcctca
     2221 tcgacctgat gttcgagatg cggctctccg cccctcaggt caatggatcc ccgcctgcca
     2281 gagaccgatc gccagtattg gagctgcgcg gcatcagcac tcgggcccag ggcgacgaga
     2341 tgaccttgag gaatctcgag ctgaagattt acccaggcga gatcgtgggc atcgccggtg
     2401 tgtccggcaa cggacagaag gaactgggag acctgatcct cggcgccaaa cgcagccagg
     2461 ccggcaccaa gctcctgttc ggcgaggatg tgacccgtaa gtcggttcgc gggttgcgca
     2521 gccgcggggt gagcttcatt cccgagaacc cattagccat ggcctccgtc ccgttcatga
     2581 cggtacttga aaacatggcc ctgacccgca cccagcaata tgcccgctgg ggcggcatgg
     2641 gaattggttg gcgagctgtc cgggatgatc tcgcccaagc atcggccgaa ctgggctttt
     2701 cgcttccgat ctttgcacct gcgaagtcgc tgtcgggcgg caatctgcag cggatggtca
     2761 tcgttcgaga gatggctcaa cgtccgaaac tgatcatcgc ttcctatctc acccgcggtt
     2821 tggacttgca aagcacctat gccgcacgcc atgcgttgat ccaggcccgg gtcgagggat
     2881 gcggggtatt gctcatttca gaggatctcg aggagctctt cacgctcagc gatcgcctga
     2941 ttgtgctcca cggcggaagg attgtgggta ctttcagacc ccaagacacc acgatctcaa
     3001 aggtgggtca tctgatgacg ggatcagagg gtcaaccttc ggacaatgtc tgattccatg
     3061 aggcgctccc catcgtgtcg agggaaggcc tggagcgccc tggttcgata tagcgtcatg
     3121 atcgtgttcg ccttcctgct cttcggcatc attttgctga ttgccggcaa tgacccaatc
     3181 cagtcgtatc gagacctctt tggctctacc ctgggcagcg cctacggtct gtctgaggtt
     3241 ttcgtcgcca tgacccccat gcttcttaca gccctggcag tggccttgcc ttcgcggatt
     3301 ggattgatca atgtcggggt tgaaggccag ctctacctgg gggccggcct cgccacttgg
     3361 ggcgcgctcg ccttcggtga tctgcccgcc tggctattgc ttccctggat gatcctgctg
     3421 gggatgatcg gcgggagcct ctgggcattg atcccgggca tcctgcgagc gatggggctg
     3481 gtcaacgaga ccatcaccac cctgctcttg aattcggtcg cccccctgat cgtgtccttc
     3541 ctagtcttcg gtttttggca ctcgcccgtg gacaccaata agacaccgaa ttttccccca
     3601 gccgcgcgcc ttccgactct gtttgatacc cgcatcgacc tgagtttggt cattggcctc
     3661 ctgctgctgg ccgtcttctg gttggcgatg aagtacagcc gatgggggct ggagatgcgg
     3721 gcgatcgggg gaaaccccca ggccgcgctg cgcaatggca tccccctgaa gacttacctg
     3781 atctcggtca tgtgcctcgg cggcgcgatc gggggactgg cggggatggc gcaagtttcc
     3841 gggttctacg gggttctgtt ggccaacttc tccaggggca tcggatacat gggtttcctg
     3901 atcagctggc tggccggcgg aaacccgctg gggattgtcc tgacctcgtt tggggtagcc
     3961 atcatcacga gtggtgggag cctcctgcag attacccgcg atgttcccta tgccgtgatc
     4021 aacatcctgc tggctttcac cctgttcgtg gttcttgccc gaccgcagct cgcgggccgg
     4081 aggaaagccg cgtgactctg ctcgcggttt tgggcgtgct gtcaagcgcc atctattcgg
     4141 ggacctccct gctttacacc accctcggcg aaatcatcga tcagcgctcg gggatcgtga
     4201 atctcgggct cgaaggcgtg ttgcttgtca gcgcttcggc cggattcgcg ttgaccgcga
     4261 tcaccggtaa cccatacctc ggcgttgcag tttcgatgct gatcggcggc gtaatgaacc
     4321 tgagcctggg ctacttgacc gtaacccgcc gtgccaacca attggccagc ggtctctcac
     4381 tgatgttctt cggcttcggt ctgagcgcat tgatcgggag accttacgtg ggcgccagga
     4441 tcgttggact gcccagactt ccgcttcctg ggctttccaa cctgccagtt ctttacagtt
     4501 cgctctttcg tttcgacctg ctcatctacc tggcgatccc cgcagccgtc ttggtgtggt
     4561 ggctgctttt ccgaacccgt tgggggctgg gactgcgggc agtgggggaa agcgccgata
     4621 tcgccttcgc ttctggtcgt gatcctcggc tgatccgata ccaagcactc ttcgtgggtg
     4681 gcttgttcgc gggcctcggc gcggctcacc tctcgatcgc ctatgccatg aactggaccg
     4741 agtacatgac cgccggacgc gggttcatcg cagttgccct ggtgatcttt tcgaaatggc
     4801 atccactccg cgcgatcgcg ggcgcgctgc tcttcagcgc tgcagtcgcc ttccagctct
     4861 tgctgcaatc gagtggaact cagatctcac catttctgct gagcacgatt ccttacctgg
     4921 tgaccatcct cgtactcatg ctctggggag gcacccgtcg gcactctgct cctgcaagcc
     4981 tagggcgggt ctatctcggc accgagtaag aaaggagagt ggaggattgt aatgaggcct
     5041 gcgattagcg aacttgggat gaccacgtga ttcggctgat gttttgagaa ccgagatgag
     5101 aggagacgca accatgagag tgaaaacctt ctattctttg ttggccgggc tcttgatgac
     5161 cgcgctcgtg ctatccgcct gcgcacaagc tgcgccgacg acagaggccc ccgcggttcc
     5221 tccggaggaa ccggccgctg aaccgacctt agagcagccg gtcgtacctg cggcggaaga
     5281 gccctgcctc atcatcggcg cgctgtacgg cgggccgatc acggatgccg gttacaacca
     5341 ggcgatgtac gaaagcgtca tgacgatcaa agaaaacatc tcttgcgtgg agatcatcga
     5401 ggccgaaaac gtccccgaag aggcgggcgc gacctcgacc atggaaaaca tggtccagca
     5461 gggtgccaaa ctgatcatcg ccactgcgtt caaccatcag tacccggccc tcgagctttc
     5521 ggcgaaatat ccggatgtga tcttcgagca cgccggcggg tgggagatgg gccccaactt
     5581 tgccaacttc ttcggcgagc ctccggatac ctggtacatg atgggcgtgg ccgctgggct
     5641 gatgaccgaa tcgaatcagc tgggctttgt cgcagccttc ccgttgggct ggactctgac
     5701 cttcatcaac gccttcgaac tgggtgccca atcggtgaat cccgacgttg agaccaccgt
     5761 gacctacacc ttcagttggg gtgacgcggc caaggaagcc gatgcgacga actcgctcat
     5821 caaccagggg gttgacgtca tcacgatgca cgtcgattcg cccgctacgg tcctgtcgac
     5881 cgcagaatcg cgggcggttt actcgattgg ctttcagtcg ctggccgccc agcagtttgc
     5941 tcccgagtac tggatctcgg gaatcggcta cacgctcggt gacaaactga ccttcctcgc
     6001 ctccacagta ttggatggga gctgggagcc gatcttcctt cgctgcggaa tagccgatgg
     6061 ctgcatggcc atggcgccgt tcggcccgaa ggtttcggcg gaggtccaag acgcggtcaa
     6121 tcaggcgaga gccgatatcg aagccggagg gatcgtggtc ttcgcaggcc ctgtggtgga
     6181 tcaggaaggg aatgtgaaga ttcccgaggg cgaagttttg agccccgacg tcatgggctc
     6241 ggtggactgg ttcgtccagg gcgtgatcgg aagtccaaag taagccacca gtcccgaaca
     6301 cttcggtggg cctcgagctc gaggcccacc gaaggacctt ggcatacccg cgccggaggc
     6361 aacctttcat ggagattaag cagaagattg agaaggccaa cgctgaagcc gtacaacgca
     6421 tgacggaggg agaaccggtc ctggttgaca tcgcgccggc aggcgaggtc attcccgggc
     6481 tgaaggacag aatgatcacc cacgccgggc cgcccatcga gtgggaacga atgtgcggag
     6541 cgcagcaagg ggccatcatc ggcatgacgc tgtacgaagg gtgggcggca aacccagacg
     6601 atgcccgcgc aatgctcgag cggggcgaaa tccgcctgga accgaaccat catcacggct
     6661 cagtggggcc gatggccggc acgatctcac cctccgcgcc ggtctgggtg gttgagaacc
     6721 gcgcccacgg aaatcggggg ttctgccgtc aggtggaggg tcggcagcaa ttcggagaat
     6781 tcagcgacgg ggccctaggg ggcttgaggc tctggcgcga cctgtgggcg ccgaccttgc
     6841 gcgaagcgct gcaggcgatt ggcggcctgg aattgaaacc gatcatcacc caggctctcc
     6901 agatgggcga cgagctgcac aaccgtcaca cggcctcctc cagcttgttc gctaaccgaa
     6961 tggctgtggc aatggcggaa gccggcattc cgaaagatcg ggcacttcca accctgaagt
     7021 acgtcacagg ccacaacctg ctttttctag ggctggccat ggcgagtggg aaagtgattg
     7081 ccgacccggc catggccatc gactgcagca cgatcgtcac cgccatggcg cgcaacggca
     7141 ccgaatttgg aatacgggtc agcggcctgg gcgaggactg gttcaccgcc ccggccccgt
     7201 cagttgaagg cttgttcctt cccggttact ccgccaaaga tgccgggctc gatatgggag
     7261 attcggctat cactgaaact gtcggctggg gagctttcac gctggccggt gcgccgggga
     7321 tcctgaccct ggtgggaggg acttcggagg aggctctggc gtacacccgc gaaatgcgaa
     7381 agatcaccac tgccctgcac cccacctacc gcatgcctgc cctgggcttt gcaggcatct
     7441 ccgtcggcat cgatattcgc aaggtagtgc agtcgaacct cgcgccgatc attgacaccg
     7501 cgatcgccca caaggatgcc ggctacccaa tcatcggcgc cgggttggtt cgggcgccca
     7561 tggaatgttt caagcaggct ttgatcgcgt ttgggaagcg gtacagcgaa gcatgatatt
     7621 tcgtaaacta cccctggagc atagcgatgg cttccggatt catagttcgt aagaaccgat
     7681 actacgattc ggtctttctg atgggcgtcg atcgtcgcct ctcgcagctg ccgggcgtgc
     7741 gccaaacggc cgtgcttatg gctagcgaga ataacaagcg cttcttggcg gagatgggaa
     7801 tacggggggc cgagctcgac gccgctcagg ccgacgacct gatagttgcg gtcatcgccg
     7861 acagccccaa gctcgtcgag caggtgttgg gaaacttgct cgacgaggca ctgatgtcgc
     7921 tcgagaccag cactcgggtc tctgacctgc gcacgctcga agagggtctt cgtgccgcgc
     7981 cgaccgcgaa cctggcggtg ctgacgatcc ccggagaata cgttgaccgc gaagcacaag
     8041 cggcgctcga atccggcctc aacctcttca tcttcagcag caatgttcct attgaagccg
     8101 agcgcaagct aaagcaattc gcgagtgaac gcaaactgct cgtcatggga ccggactgcg
     8161 ggaccagcat cttgaacgga gtcggggttg ggttcgccaa cgttgtgcg
//
LOCUS       DATDPQ010000339         6380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3579137, whole genome shotgun sequence.
ACCESSION   DATDPQ010000339 DATDPQ010000000
VERSION     DATDPQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3579137"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VI520_07650"
     CDS             <1..675
                     /locus_tag="VI520_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLF37792.1"
                     /translation="SAGEWTVPLVIRAPYGGGIGGGLYHSQSVEAFYAHVPGLKVVIP
                     SNPYDAKGLLKSAVRDPDPVMFFEPKKGYRLIRGEVPEGEEILVPIGPARVSRPGADL
                     SVFAYGMMHQYCWEAAERVAQEGIDAEVVDLRTLKPVDKQAILSSVAKTGKALIVYED
                     NLTGGYGAEIAAILAEEAFTLLDAPVRRLAGPDIPAVPYSHPLQDWFMPNPDKIAAAI
                     RDLAAY"
     gene            703..2061
                     /locus_tag="VI520_07655"
     CDS             703..2061
                     /locus_tag="VI520_07655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLF37793.1"
                     /translation="MAIEVIMPQLGESVVEGTVTKWLKRQHERIQEFEPLVEINTDKV
                     DTEVPSPASGTVLQLLVAEGETVRAGTVLALIGEPGDVVEQMAKAGTEDGEFAPSDVR
                     IAMSTPPARPAIPAPPLAGRAAVSPSEVGFISPVVARIAAERGVDLSKVPGSGQGGRI
                     TKKDVLSFLEAKKPVAPWEEPASGELFRPTEEVFGEFKPSVAGPQPRHEPPGPQGAAP
                     AAKVQPLDPVRARIAEHMVRSRHTSPHVTTVMEADLGRVLAHRSANRAAFEQDGVKLT
                     LSPYFVVAAVEALKQVPIVNSSWSDQGIVLHQQVNVGVAVSLGERGLIVPVVKNAHQL
                     SLLALARSVNDLSERARNRKLDPDEVQGGTFTITNHGVSGSLLAMPIINQPQCAILGI
                     GKLQKRVVVIENRGPAGEPSDSLAVRPMVYLTLTFDHRIIDGAIADHFLAVVVDRLQA
                     WA"
     gene            2111..3508
                     /gene="lpdA"
                     /locus_tag="VI520_07660"
     CDS             2111..3508
                     /gene="lpdA"
                     /locus_tag="VI520_07660"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLF37794.1"
                     /translation="MPDYDVIVIGAGPGGYVCAIRAAQLGLKTAIVDREWLGGICLNV
                     GCIPSKALLKNAELVHLLRTRAAEFGIGFEGLTLDYSRAVARSREAADRLVKGVGFLM
                     KKNKIEVHMGTAAFSGPHSLTVTGADGNVASLSAQHVVLATGSRPILIPGVEIDGERV
                     LTYREAVLQTTAPKSVVIVGSGAIGLEFASLWNGYGVEVTIVEMLPRIAPLEDEEVSK
                     ELTKEFRKRGINVLAGTRVEAIQTLKTKIKATVTGEAGQQVLDAEQALVAIGFRPNTE
                     GLGLELIGVALDDRKLVGIDSRMATNVPGVWAIGDVTGKLQLAHVAMAMGVVCAENIA
                     GVETIELDYQMMPRATYCQPQVASFGMTESQARQAGHELRVGRFPFQANGKALGMGET
                     AGWIKLIADAQYGEILGAHLVGPEVTELLPELTLAQMMELTPAEIARNVHAHPSLSEV
                     LGEAAHVAEGHAIHI"
     gene            3592..4770
                     /locus_tag="VI520_07665"
     CDS             3592..4770
                     /locus_tag="VI520_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HLF37795.1"
                     /translation="MSVTKGLDGVLVTESRLSRVDGLAGELTIGGFPLEELAPNAKYE
                     EVLFLLWEDRLPSASELSALQGELAALRALPGPTMDILRAAAAKSLPPMDALRMGAGT
                     LTLADPDPADSSPAANQARAKAIVARVATIVAAYWRMMHGKPAVGPRPDLPHAANYLF
                     MLRGSEAEEDEVRGLETYINSVIDHGMNNSTFTARVIISTGSDMISAVVGAIGSLKGP
                     LHGGAPGPALDMVFELRTRAAASGKSLEAEAEAYVRQTVDAGGRIMGFGHRVYKVRDP
                     RADVLGAAAEKLFKQAGDIQLNQDARAVEQVVLRVLEELKPGRGLKTNVEFYTALVLH
                     GLGFDTGLFTPTFAVGRVGGWTAQVLEQIAEARLIRPGSGYRGELNRKWVPMEKRSGS
                     "
     gene            4760..5764
                     /locus_tag="VI520_07670"
     CDS             4760..5764
                     /locus_tag="VI520_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37796.1"
                     /translation="MEAETVSQVKERFKETILRKPNVVGVGTGFKEVRGRRTTDLCVV
                     ALVRVKVPRAGLAEDELVPSRVEGIPTDVVEVGELRAEQARTDRWRPAPGGISIGHFK
                     VTAGTLGTVVRDRAGGGRLILSNNHVLANSNQAIVGDPVLQPGAIDGGSPNSDTLARL
                     ERFVEIQFNQEPPTCGIAKLVAELLNGLARLVGSRHRLRVIQENPQAVNRVDAAVAKP
                     LNSADLLPEILDIGEVQGSVAPTLGMAVRKSGRTTGFTTGQVNVIETTVTVNYGENRT
                     ARFEGQFVTTPMSQGGDSGSLLVDGETLRAVGLLFAGSAQATIYNPIQDVLEGLQIDL
                     "
     gene            5761..6036
                     /locus_tag="VI520_07675"
     CDS             5761..6036
                     /locus_tag="VI520_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37797.1"
                     /translation="MTPEQALEHARAVSRANADRLLAMANVVGVGVGYRGGTRQELIL
                     VVMVKQKLPRAQLRKEDLLPQEIEGIPVEVRVVGELRAQNASEGTAT"
     gene            6068..>6380
                     /locus_tag="VI520_07680"
     CDS             6068..>6380
                     /locus_tag="VI520_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37798.1"
                     /translation="MKPIGEQTVKALSWQLLYGNYRSKLGAEGWERLEQAVGAPAFRR
                     MDSDEDCPMAPFNEAVLFIDRQVGNGDGSMIEEITIASVERWASIFRNLVAQLQGRPQ
                     KM"
BASE COUNT         1186 a         1865 c         2236 g         1093 t
ORIGIN      
        1 tcggccggcg agtggacagt tccgctggtg atccgggcgc cctacggagg cggaatcgga
       61 ggcggtctct atcactcgca atcggtggag gccttctatg cccatgtccc ggggttgaag
      121 gtcgtgatcc cctcgaatcc gtatgacgcc aaagggttgc tgaaatcggc ggtccgagat
      181 cccgacccgg tgatgttctt cgaacccaag aagggctacc gcttgatccg cggcgaggtg
      241 cccgagggag aggagatcct ggtcccgatc ggcccagccc gggtatcccg tccgggagct
      301 gacctcagcg tgttcgccta tgggatgatg catcagtact gctgggaagc cgctgagcgg
      361 gtcgcccagg aagggattga cgcggaggtc gtggacttgc ggactctgaa gccggtggat
      421 aagcaggcta tattgagttc ggtggccaag accgggaagg ccttgatcgt ctacgaagac
      481 aacctgaccg gcggctacgg agccgagatc gccgcgattc tagcagagga ggctttcacc
      541 ctgctcgacg cgccggtgcg gcggctggcc gggccggata ttccggcggt tccgtacagt
      601 cacccgctgc aagactggtt catgccgaac ccggataaga tcgcggcggc catccgcgat
      661 ctggccgcgt attgatcgaa gaaagctgag aggaccgaac tcatggcgat cgaagtcatc
      721 atgccccagc tcggcgagtc ggtcgtcgag ggcacggtca ctaaatggct gaagcggcag
      781 catgagcgga tccaggagtt cgagccgctg gtcgagatca acaccgacaa ggtggacacc
      841 gaagtgccct cgccggccag cgggaccgtc ctgcagctcc tggtagccga gggcgagacg
      901 gtgcgggcag gaaccgtgct ggcgctgatc ggagagccgg gagacgtggt tgagcagatg
      961 gcaaaggctg gcactgagga cggcgagttt gctccgtcag atgtccggat tgcaatgagc
     1021 acgccgcccg ctcgtcccgc gatccccgcc ccaccgctgg caggtcgagc tgcggtctcc
     1081 ccgagtgaag tggggttcat ctctccggtg gtcgcccgaa ttgccgccga acgtggggtg
     1141 gacctgagca aggtgcccgg aagcggtcag ggcgggcgga ttaccaagaa ggacgtcctg
     1201 agcttcctgg aggcgaagaa gccggtagct ccatgggagg agccggcctc cggcgaactc
     1261 ttccggccga ccgaagaagt attcggggag ttcaagccgt cggtagccgg accacagccg
     1321 cggcatgagc ctcccgggcc gcagggggcc gcccccgcag ccaaagtgca gccgctggat
     1381 ccggtccggg ctcgaatcgc cgagcatatg gtgcgctcgc ggcacaccag cccccatgtc
     1441 acgaccgtga tggaggccga tctcgggcgg gtgcttgcac atcggtccgc caatcgggca
     1501 gcctttgaac aggatggggt caagctcacc ctcagcccct atttcgtggt cgctgcggta
     1561 gaggcgctga agcaagtacc gatcgtcaat tcgtcctgga gcgaccaggg gatcgtgctg
     1621 caccagcaag tcaacgtcgg agtcgccgtc tcgctgggtg agcggggcct gatcgtaccg
     1681 gtggtaaaga atgcacacca gctctcgctc ctggcattgg cccgatcggt caacgacctg
     1741 tccgagcggg cccgcaaccg gaagctcgac cccgacgagg ttcaaggtgg gacctttacc
     1801 atcaccaacc acggtgtgag tgggtcgctg cttgccatgc cgatcatcaa ccagccgcag
     1861 tgcgcgatcc tggggatcgg caagctgcag aaacgagtgg ttgtgataga gaaccgagga
     1921 cccgccggag agccgagcga cagtctggcg gtgcgtccga tggtctattt gaccttgacc
     1981 ttcgaccacc ggattattga cggcgcgatt gccgatcatt tcttggccgt ggtcgtcgac
     2041 cgacttcagg cttgggcctg aggccccgga gctccctcgc ccgcccggat tcacagagga
     2101 gaatcgccga atgccagact atgacgttat cgtgatcggg gccggtcccg ggggctatgt
     2161 ctgcgcgatc cgggccgccc agctcggtct gaagaccgcc atcgtggacc gggagtggct
     2221 cgggggcata tgcttgaatg tcggttgcat cccatccaaa gccctgctga agaacgctga
     2281 gctggtccac ctgctgcgca ctcgggcggc ggagttcggg atcgggtttg agggcctgac
     2341 tctggattac tcccgggcgg tcgcccgcag tcgggaggcg gccgaccggc tggtcaaggg
     2401 cgtcgggttc ttgatgaaga agaacaagat cgaggttcac atgggaaccg ccgccttctc
     2461 cgggccccat tcgctgaccg tgacaggcgc ggatggaaac gtggcctccc tctcggcaca
     2521 gcatgtcgtg ctcgccaccg gttcgcggcc gatcctgatc cccggagtcg agatcgatgg
     2581 ggaacgagta ctgacctatc gggaggcggt gctgcagacc accgcgccca agtcggttgt
     2641 gatcgtgggg tccggcgcga tcggactcga attcgccagc ctgtggaacg gctacggggt
     2701 cgaggtgacg atcgtggaga tgctgccgcg gattgcccca ctggaggacg aggaagtcag
     2761 caaagaactg accaaggagt ttcgcaaacg cggcatcaat gttcttgccg gcacccgggt
     2821 ggaagccatt caaaccttaa agaccaagat caaggccacc gtgaccggcg aggcggggca
     2881 gcaggtgctc gacgccgagc aggcgctggt ggcgattggc tttcgtccca ataccgaggg
     2941 actcgggctg gagctgatcg gggttgcgct cgacgatcgc aagctggtcg ggattgactc
     3001 ccgcatggcg accaacgtgc cgggcgtatg ggcgatcggt gatgtgaccg gcaaactgca
     3061 gctggcccat gtggcgatgg ccatgggagt ggtgtgcgcc gagaacattg ccggggtcga
     3121 gaccatcgag ttggattatc agatgatgcc gcgggcgacc tactgtcagc cgcaggtggc
     3181 atcctttgga atgaccgaat cccaggcccg gcaagccgga cacgagctgc gggttgggcg
     3241 cttcccgttt caggcgaacg ggaaggcgct cgggatgggg gagactgccg gttggatcaa
     3301 actgatcgcc gatgctcagt acggagagat ccttggcgcg cacctggttg gaccagaggt
     3361 gaccgaacta ttgccggagc tcactctggc tcagatgatg gagcttaccc cggccgagat
     3421 cgcccgcaac gtgcatgccc atccgtcgtt gagtgaggtg ctgggggagg ctgcccatgt
     3481 cgccgagggc catgcgatcc atatctagca atcgaagcgc tcggtcgaat cagatcggtg
     3541 cgcggtgcgg ccggcgtgcc ctttgaatcc aacccaaggg ctggaggcgt gatgagtgtg
     3601 acgaaaggtt tagatggcgt gctggtcacc gaatcccgac tcagccgagt ggatggactg
     3661 gccggcgagc tcaccatcgg tggctttccg ctggaggagc tggctcccaa cgccaagtac
     3721 gaagaagtcc tattcttgct ttgggaggac cgactgccaa gcgcctcgga gttgagcgcg
     3781 ttgcaggggg aactggccgc cctccgcgcg ctccccgggc cgacgatgga catcctgcgg
     3841 gccgcggccg ccaaatccct gccaccgatg gacgcgctgc gaatgggtgc cgggacgctg
     3901 accctggccg atccggatcc ggccgactca tcgccggccg ccaatcaggc ccgggccaag
     3961 gcgatcgtcg cccgggtggc gaccatcgtc gctgcctact ggcggatgat gcatgggaaa
     4021 ccagcggtgg ggccacgccc cgacctgccg catgccgcga actacctctt catgctgcgg
     4081 ggcagcgaag ccgaagagga cgaagtgcgc ggactggaaa cgtacattaa ttccgtcatc
     4141 gatcatggga tgaataactc caccttcacg gctcgggtga tcatcagcac gggatccgac
     4201 atgatctccg ccgtggtggg cgcgatcgga tcgctcaaag gtcccttaca tggcggcgcg
     4261 cccgggccgg cgttggacat ggtgttcgag ctacgcacgc gggcggcggc ctccgggaag
     4321 agcctcgaag cggaagccga ggcctacgtg cgccagaccg tggacgccgg tggccggatc
     4381 atgggcttcg gacatcgggt gtacaaggtg cgcgatcccc gggccgatgt actgggcgcg
     4441 gcggccgaga agctgttcaa gcaggccggc gatattcagc tcaaccagga tgcccgggcg
     4501 gtggagcagg ttgtgctgcg ggtgctggag gagctgaagc cgggccgagg gctgaagacc
     4561 aatgtggagt tctacactgc cctggtcctg cacggcctgg gattcgacac cgggctgttt
     4621 acccctacct ttgcggtggg tcgggtggga ggctggaccg ctcaggtatt ggaacagatc
     4681 gccgaggccc gcctcatccg gccggggtcg ggctaccgtg gagagctgaa tcgcaagtgg
     4741 gtgccaatgg agaagcgcag tggaagctga aaccgttagc caagtcaaag agcgcttcaa
     4801 agagaccatc ctacgcaagc ccaatgtggt cggggtggga actggattca aagaggttcg
     4861 cgggcgccgc accaccgacc tgtgtgtggt cgccctggtt cgagtgaagg ttccgcgggc
     4921 cgggctcgca gaggatgagc tggtgccctc ccgggtggaa gggattccaa ccgatgtggt
     4981 ggaagtcggc gagctgcgag cggagcaggc ccggaccgac cgctggcggc cagcgccggg
     5041 agggatcagc atcggccact tcaaagtgac cgccgggacc ctggggaccg tggtgcggga
     5101 tcgggcgggc ggcgggcggc ttatcctgtc caacaaccac gtgcttgcca acagcaacca
     5161 ggcaatcgtc ggcgatccgg tgctgcagcc cggggccatc gatggcggca gtccgaattc
     5221 ggacaccctc gcccggctgg aacggttcgt cgagatccag ttcaaccagg agccgcccac
     5281 ctgcgggatc gccaagctgg tcgccgagct gctcaatggg ttggcccggc tggtcggatc
     5341 ccggcatcgc ctgcgggtga ttcaggagaa tccacaggcg gtcaatcggg tggacgcggc
     5401 tgtggccaag ccgttgaatt cggcggatct gttgccagag atcctcgaca tcggcgaggt
     5461 ccaggggtcg gtggctccga cattggggat ggcggtgaga aaatccgggc gtacgaccgg
     5521 gtttacgact ggacaagtga acgtgatcga aactacagtg acggtcaact acggtgagaa
     5581 tcggaccgcc cgcttcgagg gccagtttgt caccactccc atgtcccagg gtggcgattc
     5641 gggatcgctg ctggtggatg gcgaaaccct ccgcgccgtg ggcctgctct tcgccggctc
     5701 ggcccaggcc acgatctaca atccgatcca ggacgtgctg gagggactgc agattgacct
     5761 gtgacccctg agcaggcact ggagcacgcc cgcgccgttt cgcgggccaa cgccgatcgg
     5821 ctgctggcca tggccaacgt ggtgggggtc ggggttggct accggggcgg cacccggcag
     5881 gagctaatcc tggtggtgat ggtgaagcag aaactgcctc gggctcaact gcggaaggaa
     5941 gacctgctgc cgcaggagat cgaggggata ccggtggagg tgcgggtcgt cggagagctg
     6001 agggcccaga atgcgagcga agggaccgca acttagcgat gccttcattt cgccgaggag
     6061 gagttcgatg aaacccattg gtgagcagac ggtcaaggca ctttcctggc agctgctgta
     6121 cggcaactac cgcagcaagc tgggagcaga gggatgggag cgattggagc aggccgtcgg
     6181 ggcgcctgcc ttccgcagga tggattcaga cgaagactgt ccgatggccc ccttcaacga
     6241 ggcggtgctg tttattgatc ggcaggtcgg caacggcgac ggctcgatga tcgaagagat
     6301 caccatcgcg tcggtcgagc gctgggccag catcttccgt aatctggtcg cccaactgca
     6361 ggggcggccg cagaagatga
//
LOCUS       DATDPQ010000340         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3354653, whole genome shotgun sequence.
ACCESSION   DATDPQ010000340 DATDPQ010000000
VERSION     DATDPQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354653"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="VI520_07685"
     CDS             <1..1326
                     /locus_tag="VI520_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLF37799.1"
                     /translation="KTAGLLVAMAQCGLHIPVAAGSELEVFQGIYVDIGDEQSIEQSL
                     STFSSHVSNIVRILRVADARSLVLLDELGAGTDPGEGAALARALLVEFLDRGCLTLVA
                     THYPELKAFAHLTDGVENASVEFDLESLRPTYHLTVGLPGRSNALAIAERLGLRKRIV
                     EAARAELSPDDLRADSMLEEIRRQREAAIQARAEAERASLEARSLRDELRRRLDGIAE
                     ERTEILGQARREAGAELEAARRQLAELRRRLTAAGEPLQSLGDIKEELEQLAAALEAP
                     DLQPLSELVDPEFDWHELAVGDRVLVRSIAAEGVLTALGGGQAEVRLGRLRIKAEPED
                     LVRLPTEPAGEQAVSEGRLPTPSAPPPLELHVRGRTVEEALEQLERSLDAAYLAGVPY
                     LRVVHGKGTGRLRQAIRQALKESGYVASFKSGAPAEGGEGVTVVWLAQD"
     gene            1337..1906
                     /locus_tag="VI520_07690"
     CDS             1337..1906
                     /locus_tag="VI520_07690"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HLF37800.1"
                     /translation="MARPIVRCWGSDDPQLAAYHDKEWGRPVHDDRVLFEHLALDGFQ
                     AGLSWRTILHKREAFREAFARFDPEQVARFGRREVQRLLKDKAIVRNRQKIEATIVNA
                     QRVLELQNELGSFDRFIWGFTGRKTLRGDPLREWRELPTRSPESEAMSAALKARGFKF
                     VGSTICYAFMQAVGMVDDHLAVCFRFKPR"
     gene            1976..>2553
                     /locus_tag="VI520_07695"
     CDS             1976..>2553
                     /locus_tag="VI520_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HLF37801.1"
                     /translation="MSKGTQVYENVSRQFNRAAELMNLDPDVRRILSEPMNEITVHFP
                     AILDDGRVEMFTAYRVQHNNVLGPFKGGLRFHPGVDIDEVRALATWMTWKTAIVDLPF
                     GGAKGGIQIDPKQFSAQELERITRRFTFALGNNIGPEFDIPAPDMNTNAQIMAWILDT
                     YLSIIPPQERQRCHHVVTGKPVDLGGSLGRDKA"
BASE COUNT          463 a          802 c          873 g          415 t
ORIGIN      
        1 aagacggccg gcctgctggt ggccatggcg cagtgcggtc tgcacatccc ggtcgcggcg
       61 ggctccgaac tggaagtctt tcagggaatc tacgtcgaca tcggcgacga gcagtcgatc
      121 gaacagtcgc tgtccacctt ctcctcccat gtctcgaata tcgtccggat cctgcgcgtg
      181 gccgacgctc gctcgttggt gttgctggat gagttgggag ccgggaccga cccaggggag
      241 ggcgccgcgc tagcccgggc gctcttggtc gagttcctgg accgaggctg cctcaccctg
      301 gtggccaccc actatccgga gctcaaggca ttcgcccatc tgaccgacgg agtcgagaac
      361 gctagcgtcg aatttgatct ggagagcctg cggccgacct accacctcac cgtgggtctc
      421 ccgggccgct cgaacgcgct ggcgatcgcc gagcggttgg ggctgcgcaa gcgaatcgtg
      481 gaggcggccc gggccgagct ttcgccggat gacctccggg ccgatagcat gctggaggag
      541 atccgccgtc aacgcgaagc ggccatccag gccagggccg aagccgagcg ggcgagtttg
      601 gaagcccgat cgctgcggga cgaactgcga cgccggctgg acgggatcgc ggaagagcgg
      661 accgagatcc tggggcaggc ccgccgggag gccggcgcag agttagaagc cgcccggcgg
      721 cagctggcgg aactccgtcg ccggctaacg gctgcgggtg aaccgctgca gtcgctcggg
      781 gatataaagg aagagctaga gcagttggcg gcggccctcg aggcgcctga cctgcaacct
      841 ttgtcggagc tggttgaccc tgaattcgac tggcacgagc tagccgtcgg tgaccgggtc
      901 ttggtgcggt cgatcgccgc cgagggagtt ctgaccgcgc tgggaggcgg acaggccgag
      961 gtgagactcg gccggctgcg gatcaaggcc gaacccgagg atctggtgcg gctgcccacc
     1021 gaaccagccg gcgagcaagc cgtgagcgaa ggcaggctcc cgacaccttc ggctccgccg
     1081 ccgctggaac tgcatgtgcg cggccggacc gtggaagagg cattggagca gttagagcgc
     1141 agcctggacg cggcctatct tgccggggtg ccctacctgc gggtggtcca cgggaaaggg
     1201 accggccggc tgcggcaggc catccgccag gccctgaagg aaagcggcta cgtggcttcc
     1261 tttaagagcg gcgctccggc ggaagggggc gagggtgtga ccgtcgtctg gttggcgcag
     1321 gattgagctg aagagaatgg ccagacccat cgtccgctgc tggggaagcg atgatccgca
     1381 gctcgccgcc taccacgaca aggagtgggg ccggccggtc cacgatgatc gagtgctctt
     1441 cgagcacctg gccctcgatg gctttcaggc cggcctctcc tggcggacca tcctgcacaa
     1501 acgggaagcg ttccgggagg ccttcgccag attcgatccg gagcaggtgg cgcgcttcgg
     1561 gcgccgcgag gtgcagcggc tgttgaagga caaagcgatc gtgcgcaatc gacagaaaat
     1621 cgaggcgacg atcgtcaacg cccaacgggt attggagttg cagaatgagc tcggaagctt
     1681 cgatcgcttc atctggggct tcaccggccg caagaccttg cgcggagatc cgctgcgcga
     1741 atggcgagag ctgccgaccc gcagccccga gtcggaggcc atgtcggctg cgctgaaggc
     1801 tcgcggattc aagttcgtcg gcagcacgat ctgctatgcc ttcatgcagg cggttggaat
     1861 ggtcgacgac cacctggcag tttgctttcg attcaagccc cgatgacgga aggcgtctcc
     1921 gatcggtcga caccggcctc ctcggccgga ccgccgaagc tccgggagta accgtatgag
     1981 caagggaact caggtttacg aaaacgtttc ccggcagttc aaccgggccg ccgagctcat
     2041 gaatctcgat cccgatgtgc gccggatcct gtccgagccg atgaatgaga tcacagtgca
     2101 cttcccggcc atcctggacg acggccgggt cgagatgttc accgcctacc gggtgcagca
     2161 caacaacgta ctcggcccgt tcaagggcgg cctgcgcttc cacccggggg ttgacatcga
     2221 cgaggttcgg gcgctggcca cctggatgac ctggaagacc gccatcgtcg accttccatt
     2281 cggtggcgct aagggcggga tccagatcga tcccaaacag ttctctgccc aggagctgga
     2341 gcggatcacc aggcgcttca ccttcgccct cggcaacaat atcggacccg aattcgacat
     2401 cccggcgccg gacatgaaca ccaatgccca gatcatggcc tggatcctgg acacctatct
     2461 ctccatcatc ccaccccagg agcgccagcg ctgccaccac gtggtcactg gcaagccggt
     2521 tgacctgggg ggcagcctgg ggagagacaa ggc
//
LOCUS       DATDPQ010000341         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_440604, whole genome shotgun sequence.
ACCESSION   DATDPQ010000341 DATDPQ010000000
VERSION     DATDPQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440604"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="paaD"
                     /locus_tag="VI520_07700"
     CDS             complement(<1..418)
                     /gene="paaD"
                     /locus_tag="VI520_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HLF37802.1"
                     /translation="MTGQHPNLDLVWQALEEVKDPELPVISIVELGLIRQVGYAAGRL
                     VVTLSPSFAGCPALDVMRQDVRQRLQQLGEESVEVRTVFSPPWTSDWIPPAGREKLRG
                     FGLAPPPRHAGDLAAVLQQAARCPYCESQDTELKNDF"
     gene            complement(415..1182)
                     /gene="paaC"
                     /locus_tag="VI520_07705"
     CDS             complement(415..1182)
                     /gene="paaC"
                     /locus_tag="VI520_07705"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HLF37803.1"
                     /translation="MKAELQEALANYLTAIGDDELILAHRDSQWAGHAPILEEDIGFA
                     NLALDELGHASIWYRLAAELRNVDPDRHPDTLVFARPASEFRNVRMVELPNGDWAFSM
                     LRQYLFDASEQVRLNALAASQYPGLAEAAAKIRTEELYHLRHTRAWVRRLGLGTQQSN
                     RKMQTALDLLYPHALQLFVEGAGELALAQAGLVPRPDRLLASWASQVSEEFIASELRL
                     PDGNLSTPAGRTAHTSHLEPLVAELQQVARLETGVAW"
     gene            complement(1179..1718)
                     /locus_tag="VI520_07710"
     CDS             complement(1179..1718)
                     /locus_tag="VI520_07710"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37804.1"
                     /translation="MKDTQWPLYQVFHQEQPDRPHTNVGAVHAPDAELALQNARDVFV
                     RRPDCVSLWVVPADQIFSLTAEEIEQAGLPPPPAGNGDSQRYLVLIKRTQAGSHEHVG
                     AVSAGSGPAALGEAVRSFGTEDALVWWVVPESLIERTDAAEVGSLFGPAKDKPYRDQA
                     FYHTTTLMRQIRGRPKQQT"
     gene            complement(1715..2644)
                     /gene="paaA"
                     /locus_tag="VI520_07715"
     CDS             complement(1715..2644)
                     /gene="paaA"
                     /locus_tag="VI520_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HLF37805.1"
                     /translation="MDEADRLEAFEERIRRGEKIEPGDWMPDEYRRQLIRMISQHAHS
                     EIVGMLPEGAWITRAPNLRRKLVLLAKVQDEAGHGQYLYHAAETLGATREEMIEALLS
                     GRAKYSSIFNYPSLSWADMGVIGWFVDGAAILNQTMLARCSYGPYSRANIRISAEESF
                     HMKQGKEQVILMSQGSPLQRSMVQDAANRWWWPTIMMFGPHDHDSPNSPVLIRWGIKT
                     KTNDQLRQDFVNQVAPEVLELGLSLPDPELHFDEASGNWLIGPIDWDEFWRVVKGDGP
                     CNAERLAARRKAHADGEWVREALAGYAERQPAA"
     gene            complement(2751..>3003)
                     /locus_tag="VI520_07720"
     CDS             complement(2751..>3003)
                     /locus_tag="VI520_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37806.1"
                     /translation="IAKFPEKTRYTKQQLNFWRDLSWHLTIGHGREWLAIHNTSPETW
                     EGVRAFGEKRPPDYDAIRRRWAEDDSPEWLGGRPPQDAR"
BASE COUNT          528 a          990 c          922 g          563 t
ORIGIN      
        1 cgaaatcatt cttcagctca gtgtcctgtg actcgcagta ggggcaccgg gcggcttgct
       61 ggagcacagc cgcgagatcc ccggcgtgcc gcggaggtgg ggccaacccg aacccgcgca
      121 gcttctcccg gccggccggg gggatccagt ccgaagtcca cgggggtgaa aacaccgtcc
      181 tgacttccac cgactcttcg cccaattgct gcaggcgttg ccgcacatcc tgcctcatca
      241 catccagggc tgggcagccc gcgaaagacg gactcagcgt gaccactaac cggccggcgg
      301 cgtatccgac ctgccgaatc agccccaatt cgacgatgga gattaccggg agctcaggat
      361 ctttgacctc ttccagcgcc tgccagacta gatccaggtt tggatgctga ccagtcacca
      421 agcgaccccc gtctccaggc gcgccacttg ctgcagttca gcgaccagcg gctccaggtg
      481 cgaagtgtgc gcagtgcgac cggccggggt ggatagattt ccatccggga gtcgcagctc
      541 agatgcaatg aactcctcgc tcacctggga agcccacgaa gccagcaacc gatcgggtcg
      601 cggcaccagc ccggcctgag ccagcgcgag ctcaccggca ccctcgacaa agagctggag
      661 tgcatgcgga tagagcaggt ccagcgccgt ttgcatcttg cggttgcttt gctgagtccc
      721 aagcccgagc cgccgcaccc aggcccgggt gtgccgaagg tgatacagct cctcggtccg
      781 gatcttggct gccgcctccg ctaagcctgg gtactggctg gcggcgaggg cgttcaaccg
      841 gacctgctcg gacgcatcaa aaaggtactg gcggagcatg ctgaaggccc agtcgccgtt
      901 tggcagctcc accatccgca catttcggaa ttccgaagcc ggacgcgcga aaaccagagt
      961 atccggatgc cggtccgggt ccacattgcg cagctcggcg gccagccgat accagattga
     1021 ggcatgtccc agctcgtcca aggccaggtt ggcgaagccg atgtcctcct ccaggatcgg
     1081 ggcatggccg gcccactgcg agtcgcgatg agccaggatc aactcatcgt cgccgatggc
     1141 cgtcaaatag ttcgcaagcg cttcctggag ttcggctttc atgtttgttg tttcgggcgc
     1201 ccgcgaatct gccgcatcag ggtggtggtg tggtagaagg cctggtcccg gtagggcttg
     1261 tccttggccg gcccgaacaa gctgccgacc tcggcggcat ccgtccgctc gatcaaactc
     1321 tcgggcacga cccaccaaac caaggcatcc tccgtcccaa agctccgcac cgcctctccc
     1381 agcgccgcag gtccggagcc cgccgagacc gctcccacat gctcgtgcga ccctgcttga
     1441 gtgcgcttga taaggaccag gtagcgctgc gagtcgccgt tgcctgccgg gggcgggggc
     1501 agacccgctt gctcgatttc ttcagcggtg aggctgaaga tctgatccgc cggaacgacc
     1561 cacagactga cacagtccgg ccgtcgcacg aagacatccc gggcattctg gagagccagc
     1621 tcggcgtcag gcgcgtgcac ggcaccgacg ttagtgtgcg gtcggtccgg ttgttcttgg
     1681 tgaaacacct gatagagcgg ccactgggta tccttcatgc cgcgggctgg cgctcggcgt
     1741 accccgccag ggcttcgcgg acccactcgc cgtcggcatg cgccttgcgg cgggccgcca
     1801 accgctccgc gttgcacggt ccatcgccct tcaccactcg ccagaactcg tcccaatcaa
     1861 tcggcccgat gagccagttt cctgaagcct cgtcgaaatg gagctccggg tcgggcaggc
     1921 tcagcccaag ctccagcact tccggggcca cctggttgac aaagtcctgc cgcagctggt
     1981 cgttggtctt agtcttgatt ccccagcgga taaggaccgg gctgttgggc gaatcgtggt
     2041 cgtgcggccc gaacatcatg atggtcggcc accaccagcg gttggcggcg tcctgcacca
     2101 tcgaccgctg caaggggctt ccctgcgaca tcagaatgac ctgctcctta ccctgcttca
     2161 tatggaagct ctcttcggcg gagatccgga tattggcccg ggagtagggg ccataggagc
     2221 agcgggcgag catggtctgg ttcaggatgg ctgccccatc cacaaaccag ccaatcacac
     2281 ccatgtcggc ccaggacaac gaaggatagt tgaagatgct cgagtatttg gcccggcccg
     2341 agagcaacgc ttcgatcatc tcttcccggg tcgctccaag cgtctcggcc gcatggtaga
     2401 gatactgccc gtggccggcc tcatcttgca ccttggccag cagtactagc ttgcggcgca
     2461 ggttgggcgc ccgggtaatc caggcccctt ccgggagcat gccgacgatc tcgctgtggg
     2521 cgtgctgcga aatcatccgg atcaactgcc ggcggtattc atccggcatc caatcgccgg
     2581 gttcaatctt ctctccccgg cgaatgcgct cctcaaaggc ctccaggcgg tcggcttcgt
     2641 ccatcactga ttcgtctgct gttggtcaat ccacggcacg gattggcgtg tcggttcaat
     2701 tatacagagc agcggtccgg gatgttctca agcgatatcg attgcacgga ctagcgggcg
     2761 tcctgcgggg ggcggccgcc gagccattcg ggtgaatcat cctccgccca tcggcggcgg
     2821 atggcgtcgt agtccggagg gcgcttctcg ccaaacgccc gcactccttc ccaggtctcc
     2881 ggactggtat tgtggatcgc gagccattcc cgaccatgcc cgatcgtcag gtgccagctc
     2941 aggtctcgcc agaagttgag ctgctgcttg gtgtagcggg tcttttcggg gaacttggca
     3001 atc
//
LOCUS       DATDPQ010000342         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_192732, whole genome shotgun sequence.
ACCESSION   DATDPQ010000342 DATDPQ010000000
VERSION     DATDPQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192732"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VI520_07725"
     CDS             <1..421
                     /locus_tag="VI520_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLF37807.1"
                     /translation="LGVWLAHTGFGLPLAVFLLFGYISTLPGEIIESARVDGASPLTT
                     FTRLVLPLSVPVVASFAIFQFLWVWNDLLVALIFLGTKEEVAVITARLAALVGRRGEQ
                     WHVLTAGAFVSMAIPLLVFFLLQRYFVRGLTAGSVKG"
     gene            437..2074
                     /locus_tag="VI520_07730"
     CDS             437..2074
                     /locus_tag="VI520_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLF37808.1"
                     /translation="MSSKPAAPWWKQAVFYQIYPLSFQDTNGNGKGDLQGIIDRLDYL
                     QDLGVDALWISPCFPSPNADWGYDVSNYTDIHPELGSLDTLDRLLQSAHQRGLRVLLD
                     FVPNHTSDQHPWFQQSRKDRENPKRDWYIWRDPGPSGGTPNNWLTYFSGSAWEWDDAT
                     QQYYLHSFLKQQPDLNWRNPEVRQAMQAALKFWLDRGVDGFRVDAVLPIIKDALFRDN
                     PTLLEPPLGKDMGPAGLQDRRHSANQPEVHALLREWRQLLNGYPGDRMMAGEVYTMDS
                     ALAAGYYGQNDELHLVLNFSLLNLPWDARLIRSFVESFEAALPTGAHATVLLGSHDEP
                     RLATRLGESQARVAAMLLLTLRGAPVLYYGDEIGMQDTPVPAHLRRDPWPEISGLSKH
                     SRDPARTPMQWDPTPGAGFSPPGTKAGELETWLPIHPDYRGRNVQVQLHEPRSMLNLY
                     RRLLRLRRSSSALHSGSYRAANETPEDCYVYYRNQGDEAWLVALNFSAEDRMLSLADP
                     RAGQITLSTGLDREEQVALSHCRLRAHEGLLVKLGPG"
     gene            complement(2139..2345)
                     /locus_tag="VI520_07735"
     CDS             complement(2139..2345)
                     /locus_tag="VI520_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37809.1"
                     /translation="MLGTGGQGAILRDAPEGAPIGFVSEGELLLVIAGPDLRATGFWW
                     LVLKEDGSQGWLVGEFLATLTPTP"
     gene            2335..>2528
                     /locus_tag="VI520_07740"
     CDS             2335..>2528
                     /locus_tag="VI520_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37810.1"
                     /translation="MPSTIAEGGVGVAVGVMVGVEVGVEVEVLVGVGVAVPVGVGVAV
                     AVGVGVGVGVGVGSEARTDVP"
BASE COUNT          438 a          807 c          816 g          467 t
ORIGIN      
        1 cctcggcgtg tggttggctc acaccgggtt cggcctgccg ctggcggtat tccttttgtt
       61 cggctacatc tcgaccctgc ccggcgagat catcgaatcg gcgcgggtag acggggcctc
      121 tcccctcact accttcaccc gtctcgtgct tccgctttcg gtgccggtgg ttgcctcgtt
      181 tgccatcttc caatttctgt gggtctggaa cgatctcctg gtggccctga ttttccttgg
      241 aaccaaagaa gaggtcgcgg tgatcacggc tcggttggcg gcactggtcg gccgacgcgg
      301 agaacagtgg cacgtcctca ccgccggcgc attcgttagc atggccattc cgctgttggt
      361 cttcttcctg ctgcagcgct acttcgtgcg cggcctgacc gctggctcgg tcaagggcta
      421 accccactcc tcccgcatgt cctccaagcc ggcagccccc tggtggaagc aggccgtctt
      481 ctaccagatc tacccgctca gctttcagga caccaacgga aatggtaagg gggatctgca
      541 agggatcatc gaccggctag attacttgca ggatctgggg gtggatgcct tgtggatcag
      601 cccgtgtttc ccctccccaa atgccgattg gggctacgac gtcagcaatt acaccgacat
      661 ccatccggag ctgggctcgt tagacacgct ggaccggctg ctgcagtccg cccaccagcg
      721 gggcctgcgg gtcttgctcg attttgttcc caaccacacc tccgaccagc acccgtggtt
      781 tcaacagtcc cgcaaggaca gagaaaaccc gaagcgcgat tggtacatct ggcgggaccc
      841 ggggccgagc ggcgggacac ccaacaactg gctcacctac ttctccgggt cggcctggga
      901 gtgggatgat gcgacccagc agtactacct gcacagcttt ctcaaacaac agccggatct
      961 aaactggcgc aatccggaag tgcgccaggc aatgcaggcg gctctcaagt tttggctgga
     1021 tcggggcgtg gacggcttcc gggtggacgc ggtgctgccg atcatcaaag acgccctttt
     1081 ccgggacaac cccacgctgc tggagccgcc tttgggcaag gatatgggcc cagccggctt
     1141 gcaggaccgc cggcacagcg ccaaccagcc tgaagtgcat gcgctgctgc gcgagtggcg
     1201 ccagctgctc aatggttacc ccggtgatcg aatgatggcg ggggaggtgt acacgatgga
     1261 ctccgccctt gccgccgggt actacggcca gaatgacgaa ctgcatctgg tgctcaactt
     1321 cagcctgctc aacttgccct gggacgcccg actgatccgc agcttcgtgg agtcttttga
     1381 ggcggcgctt ccgacggggg cacatgcgac ggtactgctc ggaagccacg acgagccgcg
     1441 cctggccacc cggctgggag aatcgcaggc cagggtggcg gcgatgctgc tgctcaccct
     1501 gcgcggggcc cccgtcctct actatggcga cgagattggg atgcaggata cgcccgtccc
     1561 agcccacctg cgccgcgatc cctggccaga aatctccggt ctgtcaaagc attcgcgcga
     1621 cccggcccgc accccgatgc agtgggaccc gactccggga gccggcttca gcccgcccgg
     1681 gacaaaagcc ggcgagctgg aaacctggct gcccatccac ccggactacc gggggcgcaa
     1741 cgttcaggtt cagctgcacg aaccgcgctc gatgctcaat ctctaccgga ggctgctgcg
     1801 gctgcggcga tcctcgtccg ccctacactc gggatcctac cgagcggcga acgagactcc
     1861 ggaggactgc tacgtatact accggaacca gggagatgag gcctggctag tggcgttgaa
     1921 cttcagcgcc gaagatcgaa tgctgtcgct tgctgatccg cgggcgggcc aaatcacgct
     1981 ctcaaccggg ttggaccggg aagagcaagt cgccctcagc cattgccggt tgcgggccca
     2041 cgaagggctc ctggttaaat tgggaccagg ctgagcttcg cttaagaagg gtcgcggtgc
     2101 gtcgctcggc gagctaaggc cgggagaggg ccgagctttc agggtgtcgg ggtcagcgtc
     2161 gccagaaatt cccccaccag ccagccctgc gatccgtctt cctttaagac cagccaccag
     2221 aagccggtgg cgcgcaagtc cggaccggca atcaccagca gcagctcacc ttccgataca
     2281 aagccaatcg gcgcgccttc cggcgcatct ctcaagatcg caccctgtcc gccggtgccc
     2341 agcacgattg ccgagggtgg ggtgggagtg gcggtcggag tgatggtcgg ggtggaggtc
     2401 ggggtagagg tcgaggtgtt ggtcggggtc ggagtggcgg taccggtcgg cgtcggagtc
     2461 gcagttgccg tcggggtggg ggtcggggtc ggcgtgggag tcggcagcga agcgaggacc
     2521 gacgtacc
//
LOCUS       DATDPQ010000343         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3890872, whole genome shotgun sequence.
ACCESSION   DATDPQ010000343 DATDPQ010000000
VERSION     DATDPQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890872"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VI520_07745"
     CDS             complement(<1..344)
                     /locus_tag="VI520_07745"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HLF37811.1"
                     /translation="MSNNSRWPAPGLQLSKSEARRFILAHQMLWPPRRLQGKDGILDF
                     VRRVGAIQFDPVNVVGRNPDLVLQSRVNRYHPGLLEEMLYRDRQLVDGWDKVASIILT
                     SDWPRFARHRIR"
     gene            complement(334..1071)
                     /locus_tag="VI520_07750"
     CDS             complement(334..1071)
                     /locus_tag="VI520_07750"
                     /inference="COORDINATES: protein motif:HMM:NF044502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HLF37812.1"
                     /translation="MERFELQLKRNSRQDVRSIIEQAAKSLGLVTTQVTTLSTFPGSI
                     HWHFKQANGRGTLEATYWPDRRQAWLSVRPGREAEWIAPAIRALKRLVAKGAKAHRKR
                     LYSIRRSPIHGRGVFALRRIPKGTRVIEYRGERISQEEATRRYEETMEYPFTYLFEVD
                     EETIIDGQVGGNTARYINHSCSPNCESLNSGGRIFIEAIRDIRPGEELTYDYRLETEE
                     PMTRRLQKMFACNCGTRRCRGTLLNVE"
     gene            complement(1409..1627)
                     /locus_tag="VI520_07755"
     CDS             complement(1409..1627)
                     /locus_tag="VI520_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37813.1"
                     /translation="MSEEQAPQQPRHSRQIEVVAEATTVQGWRKQVVAGQRGEKLFTF
                     FSDEGSYMPGGQGTAPTPLTYFVSGVAL"
     gene            complement(1728..>2403)
                     /locus_tag="VI520_07760"
     CDS             complement(1728..>2403)
                     /locus_tag="VI520_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HLF37814.1"
                     /translation="LYSGYKTEEGVDPDLLAQFPLAERAVAALGIVVWPMVEFEADDA
                     LASATHRFQRRRSVEQVVLCSPDKDLAQMVSGRRVVSWDRRREIVYDEAAVIQKYGVP
                     PASIPDWLALVGDTADGYPGIPAWGEKSTSAVLSKYKHLEAIPEDHRKWGLSAGRALR
                     LAENLRKQWKDALLFRRLATLRTDVPLEQNLKDLQWRGARKRLRKLCKELGAGRLLDR
                     VPRWVD"
BASE COUNT          445 a          729 c          737 g          492 t
ORIGIN      
        1 atacggatcc gatgccgggc gaaacgcggc cagtcgctgg tgaggatgat cgaggcaact
       61 ttgtcccagc cgtccaccag ctgacggtcg cgatagagca tctcctcgag cagaccagga
      121 tggtagcgat tcaccctgga ctgcagcacc aggtccggat ttcgccccac cacgttgacc
      181 ggatcgaatt ggattgcgcc gacccgccgg acgaaatcga gaattccgtc tttaccttgc
      241 agccgccgcg gcggccagag catctggtgg gccaggatga atcggcgcgc ctcacttttg
      301 ctcagctgca ggccgggggc cggccagcgc gaattattcg acattcagca gagtgccccg
      361 acagcggcgg gtaccgcagt tgcaggcaaa catcttttgc agccggcggg tcattggttc
      421 ttcggtctcc agccggtagt cataggttag ctcttccccg ggccggatat cccgaatcgc
      481 ctcgatgaag attcgcccgc cggaattcag cgactcgcaa ttcggcgaac aggaatggtt
      541 gatgtagcgg gcggtgttgc cgcccacctg gccatcgatg atcgtctctt catcgacctc
      601 gaacaggtag gtaaacggat actccatggt ctcttcatat cgccgggtcg cttcctcttg
      661 cgaaatccgc tcaccccgat actcgattac ccgagtgcct ttggggatgc gccggagggc
      721 gaagacccct cgaccgtgga tcggcgatcg acgaatggag tagagccgct tgcggtgggc
      781 cttcgcacct tttgcgacca ggcgcttgag ggcccgaatg gcaggggcaa tccactcggc
      841 ttctcgaccc ggtcgaacgg agagccaggc ctgtcgccga tcgggccagt aggtcgcctc
      901 caaggtcccg cgaccattgg cctgcttgaa gtgccagtgg atggagccgg gaaacgttga
      961 gagggtcgtc acctgggtgg tcaccagacc aaggctcttg gcagcctgct caatgatgga
     1021 tcggacgtcc tgccgcgagt ttcgcttaag ttggagctcg aagcgttcca aactaatact
     1081 caaccttcca ggcgtggcgg atttctcact cggacctgcg aacctcaatg gggctcccgt
     1141 tcagatggtg gcttgttgaa atctggacgg cctcagataa ggtgtattca gtgaagcaca
     1201 tgcgatgggc ttggcggatc aacctgacaa ccgcttcttc tgattcgtca ctgtcgagaa
     1261 agagctcgat ggagaagcct tcgcacgttc cgtgttgggt cccccccaag acggatccct
     1321 cttctcggaa ttgacccacg acttttacgc actcgttccg taagacaagt cttcgcttgg
     1381 ccgcaacctg cgacacttgg gagagcagtc agagggcgac gcccgagacg aagtaagtca
     1441 agggcgtagg cgccgtgccc tggcctccgg gcatgtagga gccttcatca ctgaagaacg
     1501 tgaaaagttt ctcaccgcgt tgaccggcga ctacctgctt gcgccaacct tggaccgtcg
     1561 tcgcctcggc gaccacttct atttgacggg agtgcctggg ctgctgtgga gcttgttctt
     1621 cactcatgat ggcaggatct cctcattgag caggcgtcgg agggccgcgc tggcgtctgt
     1681 gggctgcgag tcgagggtgt cggccactcc ctcgtggtcg actatggcta gtcgacccag
     1741 cgaggcactc gatcgagcag gcggccggcg cccagttcct tgcagagctt tcttagtcgt
     1801 tttcgcgccc cgcgccattg caggtctttc aggttttgtt cgagcgggac gtcggtgcga
     1861 agcgtcgcca ggcggcggaa gagcagggcg tccttccact gcttcctgag attctcggcc
     1921 aggcggaggg cgcgaccggc ggacaggccc cacttgcgat gatcttccgg gatggcctcg
     1981 aggtgcttgt acttggagag cactgcggaa gtggacttct cgccccacgc ggggatcccc
     2041 gggtagccgt ctgcagtatc acccactaag gcgagccagt cggggatgga agctggcggt
     2101 actccatact tctggatgac cgcagcttca tcataaacaa tctctcggcg gcggtcccaa
     2161 ctcactaccc tcctccccga gaccatttgg gccaggtctt tgtctgggga acacaggaca
     2221 acttgctcga cggagcgcct gcgctggaaa cgatgagtcg ccgaggcgag cgcgtcatcg
     2281 gcctcgaact cgaccatcgg ccagacgacg attcccaagg ccgccaccgc cctctcggca
     2341 agcgggaact gggccagcag gtccgggtcg actccttcct cagtcttgta tcccgagtag
     2401 agg
//
LOCUS       DATDPQ010000344         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_194020, whole genome shotgun sequence.
ACCESSION   DATDPQ010000344 DATDPQ010000000
VERSION     DATDPQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194020"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VI520_07765"
     CDS             complement(<1..225)
                     /locus_tag="VI520_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HLF37815.1"
                     /translation="MGDYEKPSPSDSELIARAKGGQVEAFGQLYERYLDPIYRFIRMR
                     VDGERDAEDLCETVFLRTYEALDRYEDRGHP"
     gene            459..1865
                     /locus_tag="VI520_07770"
     CDS             459..1865
                     /locus_tag="VI520_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37816.1"
                     /translation="MISLTGKQLGAYQIVERIDTRPGSDAARPSESRVYRATPSGGGE
                     AVAIKVFSANPLSPESRTRLRDSLRVVSQLRHSGAAPILGSGVVEGQPYIVMPYFTAG
                     SLADRYERGLASSLDPSELLNELSAVLDVAHQRGIVHGSLRPSSLLFDPESGSVRLVG
                     LGESVGYSTEKAQTAAAGAIYAAPEVTAGQDPTPGADQYSFGVVAAEMLTGLPAEHAL
                     GYLRARSVGRPYENRNLRTPLTAQTLLALSKATAAAPGARFATVGEANRSLQIALGKL
                     APVIAPEPAEVPEAPRIRPRRRRAAAAVFVVLLLALVGIVPALAAGLIELPSWAGGSR
                     VGATSDGQLSSGPTIMLPTENQPPTGPGDPGYALGGESTTPSVGDAAQPVSGVGSTVT
                     DPLNPTATPDAGSQLPGPTSSSAAPTPTNPAPPTSAPTSTPPPTVAPTNPPPTAPGIK
                     KCKDDPAHPNYCTPTPSP"
     gene            1914..3392
                     /locus_tag="VI520_07775"
     CDS             1914..3392
                     /locus_tag="VI520_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLF37817.1"
                     /translation="MRNSRLFTTIAAISIALTPVSALAAGELEVLVEVSVTCDVATFQ
                     AGVVEGVSSSYTFDWDFGDLETLTLVNQTANPGPVVHPYPSAGEYDWSLSVMDDGEGT
                     GEASGTVLIDGPIVTLSSDPFPPLLVLQGGTATANFTAVVEGGLAPYDYAWDLDGDGL
                     ADLEGSPGPASFTYTEAGKYQASVLVVDECGLAGEASLPVVVIDPEAEACHPMAQRIA
                     DAVNTLFPNQAGTLYTCEDIFAIFQGSLTGSQVGFGRLWHAYKLAQTMEDLTWEEIRD
                     WHLAGNGWGLLVQLDRFADTLDEVGLRELLDRVLSGEASVGDIRQAVRAVVRYEVDFE
                     DALARLADGASPGELGRLYRTAQDLGVEPNALDQYLTDGVSLAELNHASRLASRTGSD
                     WQQVLEDHASGQSWGEINQAERSANSDGTSNSESSPKDEHDQLREEAQGERDANRDQK
                     MATRLAKQFGINESEVWVLFSGTCAGDWGCVRSTLRQGSNGR"
     gene            3440..>3667
                     /locus_tag="VI520_07780"
     CDS             3440..>3667
                     /locus_tag="VI520_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37818.1"
                     /translation="MAGLTGTRLGPYQILEQIGRGGMAAVYRAHDTARDRFVALKLVA
                     PHLTNDPNFEQRFRREARLLMQLKHPNIIPVE"
BASE COUNT          691 a         1195 c         1135 g          646 t
ORIGIN      
        1 tgggtggccc cggtcctcat agcggtccag cgcttcatag gttcgcagaa aaacagtctc
       61 acagaggtcc tccgcgtccc gctcgccgtc gacccgcatc cggatgaaac gataaatcgg
      121 atccaggtag cgttcataca gctgaccgaa cgcctcgacc tgcccgcctt ttgcccgggc
      181 gatcagctcg gagtcgctgg gtgacggctt ctcgtaatca cccatgagaa cgagtgaggc
      241 ttcgcattgt tacaggcggc tcggcatacc taacggcggt aggactacgg tcatggggca
      301 gccctgtaac agtaaagcat ttccccggtt ctccttatag gaccgcaacg ccatgcgcag
      361 acggagctat tggcgctggc ccaagttcaa cggtttcgcc gagcgggcat tctaagcact
      421 actgagacag cggagaccag aatcctgacc cacctcgcat gatatcgctg accggaaaac
      481 aactgggcgc ttatcagatc gtcgagcgga tcgatacccg cccaggatca gatgccgcgc
      541 gtccatcgga gtcgcgggtt taccgagcga ctccttcggg cggtggggag gccgtcgcga
      601 tcaaggtgtt ctcggcgaac ccgctctcgc cggagtcccg gacccgcctg cgcgattcac
      661 tgcgggtggt ctcccagctt cgccactccg gtgccgcccc aatccttggc tccggggtcg
      721 tcgaggggca gccctacatt gtgatgccct acttcactgc gggcagcctg gctgaccggt
      781 atgaacgagg actcgccagc tcgcttgacc ccagcgagct gttgaatgag ctctccgccg
      841 tgctggacgt ggcccaccaa cgagggatcg tacacggctc gctcaggcct tccagcctcc
      901 tgttcgaccc ggaaagcggc agcgttcgac tcgtaggctt gggcgagtcg gtcggctact
      961 ccactgagaa agcgcagact gccgctgctg gcgcaatcta cgccgcgccg gaggttaccg
     1021 cggggcagga cccaaccccg ggcgcggatc agtactcgtt cggggtcgtg gctgcagaga
     1081 tgttgaccgg tctgcccgcc gaacatgcgc tcgggtatct gagggcccgg tcggtcggcc
     1141 ggccttacga gaaccggaac ttgcggactc ccctgactgc ccaaaccctg ctggccctct
     1201 cgaaagcgac ggcggccgcc ccaggagcgc gcttcgcgac cgtcggcgag gccaaccgca
     1261 gtttgcagat cgcattgggc aagctggctc ctgtgatcgc gcccgagccg gccgaagttc
     1321 ccgaagcgcc tcgcatccga cctcgtcggc ggcgggccgc agcagccgtt ttcgttgtcc
     1381 tgctgctagc cctggtcggc attgttccgg ccctggcggc tggcctgatc gaactgccaa
     1441 gctgggcagg cgggagccga gtcggggcaa caagcgacgg ccagctctca tccggcccca
     1501 cgatcatgct tcccaccgag aatcagccgc caaccggtcc aggcgatcca gggtacgcgt
     1561 tgggcggcga atctacaact ccgtcggtcg gggatgccgc gcaacccgta tcgggtgtcg
     1621 gttcgaccgt gaccgatccg ctgaatccca ccgccactcc ggatgcgggc tctcaacttc
     1681 caggccccac ctcaagctcg gccgcgccga ccccgacgaa tcctgcgccg ccaacctcag
     1741 ccccgacgag tactccgcct ccgaccgtag cccccaccaa cccgcctccc acggcgccgg
     1801 gcatcaagaa atgcaaagac gatccagccc atccgaacta ctgcactccg accccctctc
     1861 catgatcgag agcgggagtc cgaccgtcaa atctgacact ggagggaact aggatgagaa
     1921 actctcgact gttcacgaca atagcagcca tatcaattgc actgactccg gtctcagccc
     1981 ttgcggccgg tgaactggag gtgctcgtgg aggtctccgt cacgtgcgat gtggccacct
     2041 ttcaggccgg cgtcgtcgaa ggggttagct cctcttacac cttcgattgg gactttggcg
     2101 acttggagac cttgaccctg gtaaaccaaa ccgccaaccc gggtcctgtc gtgcatccct
     2161 atccgtccgc cggcgagtat gactggtcgc tttccgtgat ggatgacggc gagggcaccg
     2221 gcgaagcaag tgggactgtc ctcattgacg gcccgatcgt taccctgagc agtgacccgt
     2281 tcccacccct gctggttctg cagggcggga cagccaccgc caacttcacc gcagttgtgg
     2341 agggaggact ggctccgtac gactatgcct gggatctgga cggcgatggc ctagccgatc
     2401 tggagggttc cccgggtcca gcgtctttca cctataccga agccgggaag taccaggcga
     2461 gtgtcctcgt cgtagatgag tgtggtctcg cgggcgaggc cagcctgccg gtcgtggtca
     2521 tcgaccccga ggcagaggcc tgccatccga tggcccagcg gatcgcggat gcggtcaata
     2581 ccctgttccc caaccaggcc ggaacgctgt acacctgcga agacatcttc gccatcttcc
     2641 aaggttccct caccggatcg caagtcggat tcggacggct ctggcatgcg tacaagcttg
     2701 cccagacgat ggaagacctg acctgggaag agatccgcga ctggcacctg gccgggaacg
     2761 gatggggcct cttggtacag ctggatcggt tcgcagacac gcttgacgaa gtaggtctga
     2821 gggagctgct tgatcgggtg ctcagcggag aggcgagcgt tggcgacatt cggcaagccg
     2881 tgcgcgccgt cgtccggtat gaagtggact tcgaagacgc gttggccagg cttgcagatg
     2941 gcgcgtctcc gggcgagctg ggtcggctgt atcggactgc ccaggatcta ggagttgagc
     3001 ccaacgcgct ggaccagtac ctgactgatg gcgtcagcct ggcggaactg aaccatgcct
     3061 cacggctggc ctcacggact gggagcgact ggcagcaggt gctggaggat catgcctccg
     3121 gtcagagctg gggcgagatc aaccaagccg agcgctcagc caacagtgac ggtacgtcga
     3181 actccgaaag cagcccgaag gacgagcatg accagttgcg ggaagaggcc caaggggaac
     3241 gcgacgcgaa ccgtgaccaa aagatggcca cccgactggc caaacagttc ggaataaacg
     3301 aatccgaagt gtgggtgctc ttcagtggca cctgcgccgg ggattggggc tgcgtccggt
     3361 ccaccctgcg ccagggctcc aatggccggt agggtctact ccaggaattt gagcggagcc
     3421 aggcgagact aacttcagca tggccggact caccggaacc cgactcgggc cctaccagat
     3481 ccttgagcaa ataggccgcg gcggaatggc cgcggtctat cgcgcccatg acacggctcg
     3541 agaccgattc gttgcgctca agctggtcgc accccatctg accaatgacc cgaatttcga
     3601 gcagcggttc cgccgcgagg cgcggctgct catgcaactc aagcacccca acatcattcc
     3661 ggtcgag
//
LOCUS       DATDPQ010000345         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3892752, whole genome shotgun sequence.
ACCESSION   DATDPQ010000345 DATDPQ010000000
VERSION     DATDPQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3892752"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VI520_07785"
     CDS             <1..2035
                     /locus_tag="VI520_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HLF37819.1"
                     /translation="LVPPDQREAARTFIDGVPKQAGVVLAGLILAFGNRSLLPTQLYL
                     LFAGIAAGGLWVFWRARHAYRGALAQALHAGNPQIFYASEQPFGGFQHDAAAIAAAVN
                     GVSDPDPAIRRVSAEILGNLEVPEASATLVAGLEDPDPIVRVALLRSLGRAGAQGALL
                     KVVAALDDSEPEVRLEAVQTIRALARYRLGRDRLGLLTQVERLLEDPAPEVRSCVAVI
                     LLEAGPHPEASRVLRDMVGTAEPQARKAVLDSIARWGDLAGFELALGCIDDPHPSVRR
                     AAVSALARSDPQGGLPRLVQLLGDGDRSVREAVAARLGELSSQALGPVLEALDDPRLA
                     DGALLALQSLPARRELPGLLGFAQDKARLAQHYLGLWRGASAIGNPSPASELFSDALI
                     YSARHAARQAILAVGLQKDPAAASLALDNLDSREAGQRANAMELLDSLGERKLVGPLL
                     QIWEAQRVLAAAGSEKEWLERGLEDTDPWVRACAALAAGESQDPALEDQLRRRVQSDP
                     DSLVRETALRSLNGGVIMETLPTRSTMERILFLRRVPLFADLSPDELKQVALVTSELS
                     FSPSEILARQGEAGDEMYIILEGEIEVSVDGTAGNREVLARRGPGEVVGEMSIISREP
                     RMASLIAASELRILRIAQKEFEGILRERPQTGLAVMRVLIKRLKEAQRSEAAP"
     gene            complement(2336..2908)
                     /locus_tag="VI520_07790"
     CDS             complement(2336..2908)
                     /locus_tag="VI520_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37820.1"
                     /translation="MFSNPVFPLGLQLGSEVELGVTARVAVGVAEGPGEPVVEVGARV
                     GVRVAVDVASRVGVRVAVGDGVRVAVGEGVRLAVGDGVREEVLVGVGVKVKVGVLVKV
                     GVRLGLGVAVGEVVSSAVAGDVAVGLSIGACGGAGINPESGTAGGRKVGSAVGLGARV
                     GVAGAGNRLIAIWRRTSELTGASRIAWVEA"
BASE COUNT          486 a         1029 c          924 g          520 t
ORIGIN      
        1 cctcgttccc cctgaccagc gggaagcggc ccgaaccttc atagacggcg tccccaagca
       61 ggccggtgtg gtgctggccg ggttgattct ggctttcggc aaccggagcc tgctgccgac
      121 ccagctctat ctgctcttcg caggtatcgc cgctggcggg ctctgggtgt tctggcgtgc
      181 ccggcacgcc taccgcgggg cgttggccca ggccctgcat gccgggaatc cgcaaatctt
      241 ctacgcctcg gagcagccgt ttggaggttt ccagcatgac gcggcggcaa tcgccgcagc
      301 cgtgaatggg gtgtccgatc cagacccggc gattcggcgg gtctcagctg agatcttggg
      361 aaatttagag gttcccgagg ccagtgccac tctggtcgcc ggattggaag atcccgaccc
      421 catcgtacgg gtcgcgctgc tgcgctcgct gggccgggcc ggggcccaag gagcgctttt
      481 gaaggttgtc gcagccctcg atgattcaga gccggaggtc cggctggagg ccgtacagac
      541 aattcgggcc ctggctcgct atcggctggg tcgcgatcgg ctggggctgc tgacccaggt
      601 tgagcgtcta ttggaagatc ccgctcctga ggttcggagc tgcgtggcgg tcatcctgct
      661 cgaggcaggt ccgcatccgg aggcgagccg cgtattgcgc gacatggtcg ggaccgccga
      721 gccgcaagcc cggaaagcgg tgcttgactc gatcgcccga tggggagact tggccggttt
      781 cgagctggcg ctcggttgca tcgatgaccc tcatccttct gtccggcgag cggcggtaag
      841 tgcgcttgcc cgcagcgatc cgcagggggg actgcctcgc ttggtccaac tgctgggcga
      901 tggggaccgg tctgttcgag aggccgttgc tgcccggctt ggggaactca gttctcaagc
      961 gctcggtccc gtcctcgaag cactcgatga ccctcggctg gcagacgggg ccttgcttgc
     1021 gttacagagc ctccctgctc ggcgggagct acccgggctg ctcgggtttg ctcaggacaa
     1081 ggcccgcctg gcgcagcact acctggggct ttggaggggc gcaagtgcaa tcggcaaccc
     1141 cagtccggcg tctgaactct tctccgatgc actgatctac agtgcgcggc atgcagctcg
     1201 tcaggctatt ctcgcggtgg gattgcagaa agaccccgcc gcagcttccc tcgcacttga
     1261 caatctggac agccgggagg ctggccagcg ggcgaatgca atggagttgc tcgattcgct
     1321 cggcgagcgc aaactggtcg ggccgctgct ccaaatctgg gaagctcagc gagttcttgc
     1381 cgcggccgga tccgagaaag agtggctgga acgcggcttg gaagacacag atccatgggt
     1441 ccgggcctgc gcggcgctgg ccgctggaga atcgcaggac ccggctctgg aggaccagct
     1501 gcgacggcga gtccagtccg accccgactc cctggttcgt gagaccgccc tcagatccct
     1561 caatggaggt gtaattatgg aaaccctgcc gacccgctcc accatggagc gaatcctgtt
     1621 cctgcgccgg gttccgctct tcgccgatct ctcgccagat gaactcaagc aggtcgcgtt
     1681 ggtgacgagt gagctctcat tcagcccgtc cgagatcctc gcccggcagg gagaggcggg
     1741 cgatgagatg tacatcatcc tcgagggaga gattgaagtc tcggtcgacg gtaccgccgg
     1801 caaccgggag gttctggccc gccgcggccc gggagaggtt gtcggcgaga tgtcgatcat
     1861 cagccgcgaa ccgcgcatgg cctccttgat cgcggccagc gaattgcgta ttctgcgcat
     1921 cgcccagaaa gaattcgagg gaatcctgcg cgagcggccg cagaccggct tggccgtgat
     1981 gcgggtgctt atcaaacgcc tgaaggaagc tcaacggtca gaagccgcac cgtagcggat
     2041 acccggcttt tgccagtgct ggtttgagct aagagaggcc gttccgggcg gaattcactc
     2101 gcgggcgccc tggtaccgcc cggctatggt tggcctttcc ggctggggcc gataattgca
     2161 ggacatgccc acaccgggac catcgtctcc ggttgagcgg ggcatcccca atggacgaaa
     2221 tcacccgccc cggccggccg cgccgacctc gccgcagcca tcccgttcgg acgaccctgc
     2281 tgatcgggct ggcgaccatt ccggtcctct cggcattgac ctttgccggg ttggcctacg
     2341 cctctaccca cgcgattcgg ctggcgccgg tcagctccga agtcctgcgc cagatcgcaa
     2401 tcaatctatt ccctgctccg gcaacgccca ccctcgcgcc aagcccgacc gcgctgccaa
     2461 ccttcctacc tccggccgta ccggattcag ggttgatccc tgccccgccg caggccccga
     2521 tcgagagccc gaccgcaaca tcacccgcga cggctgaact cactacttca ccgacggcga
     2581 ccccgagccc aagcctgacc ccgaccttta ccagcacgcc cactttcact ttcaccccca
     2641 ccccgaccag cacctcctcc cgcacgccat caccgaccgc cagccgcacc ccctcgccca
     2701 ccgccacacg cacaccgtcg cccacagcga ctcgcacccc cactctgctg gcgacgtcga
     2761 ctgcaacccg tacgccgacc ctcgcgccga cctcaactac cggctccccg gggccctccg
     2821 cgacccccac ggcaaccctc gccgtcaccc ccagctcgac ttcggatccg agctgcagcc
     2881 cgagcgggaa caccgggttc gaaaacacat tgctggggct gatcaaccag gagcgcgccg
     2941 cgcagggcct gccggccta
//
LOCUS       DATDPQ010000346         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1516090, whole genome shotgun sequence.
ACCESSION   DATDPQ010000346 DATDPQ010000000
VERSION     DATDPQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516090"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VI520_07795"
     CDS             complement(<1..1010)
                     /locus_tag="VI520_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37821.1"
                     /translation="MATMAATAEVFTRKASGLVRVMSPYSAFVYNILTMGIIFPWVFL
                     VSPTAFPGGSIPLGILICTLIQIPIALSAVWLSTALPRSGGDYVFQSRVLGGGWGFTL
                     VMSGFVIWILVWTALSGWLVGVLGLAPLLLGLGNVLGSAGLISAGLWVTTPLGLFVVS
                     LINGGASAALLVSGFKNYVRFQHVIWYSILIGFAAALLILFGSSAGVFPQRLNEFAAA
                     VGDSPTFYQDTLATAQGAGVNTAPSFSWLLTLLVAPVAWQSLQWSTYSAQQNGEIKEA
                     RSFRNQAFIMVGSLIVTGLLLALLGVALQNAAGTEGQVVASYGYWYVDPTAKIAGTII
                     LAP"
     gene            complement(1099..2094)
                     /locus_tag="VI520_07800"
     CDS             complement(1099..2094)
                     /locus_tag="VI520_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37822.1"
                     /translation="MSLFPRKPARKTTRLFFATDVHGSDRTFRKFINSAKFYEAAVLV
                     MGGDILGKLAIPIIQEANGRYRATLQGRTETIEGESGLEQLRSRLGTLGFYSKVMDEA
                     EFRRIQSEPAAVEALFKELARQRLEAWVELAETRLAGSGVRCFVTGGNDDYTEVLDVL
                     YRGGTQAIFGCEGKLVNLDDDHTLISVGYSTPTPWHTPREVSDDQLGEMIEAAVRQSP
                     DLSHCIFNFHDPPVDSSLDTCPMLDWSTDPPTQIVKGGQVVLFGAGSASVRRAIESHQ
                     PVLGLHGHIHESPGVINIGRTLCVNPGSEYGEGILRGVLVSLADGEVTGHQLTSG"
     gene            complement(2107..2931)
                     /locus_tag="VI520_07805"
     CDS             complement(2107..2931)
                     /locus_tag="VI520_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HLF37823.1"
                     /translation="MQVSQPLIPAQRRHRIREFLQRHQIVRSATLSEILGVSEATVRR
                     DLVWLEREGEVERTHGGALLTQRLPIEPAYASSALAHPDEKRRIGATAAALAEPGDTI
                     FVNSGTTTAEVLRQLGARGLTDLTVVTNNLAASLEAREPGFELILVGGSFRPRSNSVS
                     GRFAAGLIGQVFADKAFIGVDGISLHHGCTTPINTEAEVAGLMIERTQGPVIIVADHS
                     KWGVVSNFAVAPLSKVSTLVTDEGLAPEWRTALRARSIEVRIAAAERPDGQEQAAP"
     gene            3066..>3692
                     /locus_tag="VI520_07810"
     CDS             3066..>3692
                     /locus_tag="VI520_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37824.1"
                     /translation="MSSSAIGMGNQGQEERQRFEAEVRRIVEASEGGGVTLRLLGSLA
                     FHAHCPQYGYLQAALGRAYTDLDFGGYVRQAKGVGQILTALGYQEDREVFVVTEGGRA
                     IFEHPGNGLHVDVFYEKLDFCHIIPWQARLELDSPTLPLAELLLEKMQIVEINEKDVI
                     DTIMLLLEHPLGVGDAETINLDRIATLCGGDWGLWRTTSMNLGKVRTLA"
BASE COUNT          720 a         1140 c         1170 g          662 t
ORIGIN      
        1 ggggccagga tgatcgtacc ggcgatcttg gccgtcgggt cgacatacca gtagccatag
       61 gaggccacca cttgcccttc cgtcccggct gcgttttgca gcgccacccc gagcaatgcc
      121 agcagcaggc ccgtgacaat cagagacccc accatgatga aagcctggtt gcgaaaggag
      181 cgggcctcct tgatttcgcc gttctgctgg gccgagtagg ttgaccactg caggctctgc
      241 cacgccaccg gtgcaaccag cagcgtgagc agccagctga aggacggcgc ggtgttcacc
      301 ccggcgccct gggccgtagc caaagtatct tggtagaaag tcggcgagtc accgaccgcg
      361 gccgcgaatt cattcagccg ctgtgggaac acacctgctg agctgccgaa aagaatcaac
      421 agggcagccg cgaagccgat cagaatgcta taccagatca cgtgctggaa gcggacgtag
      481 ttcttgaatc cactcacgag cagggcggcc gaggcacccc cattgatgag actgaccacg
      541 aacagtccga gcggcgtagt aacccacagc ccggctgaga tcaggccggc actccccagg
      601 acattgccca ggcctagcag caagggggcc aggcccagta ccccgaccag ccagccggag
      661 agagcggtcc acaccaaaat ccagattacg aacccgctca tcacgagggt aaagccccag
      721 cccccgccca acactcggct ctggaagaca tagtcgccgc cggagcgcgg tagggcagtg
      781 gagagccaga cggccgagag cgcgatcgga atctggatca gggtgcagat caagatcccc
      841 aggggaatgc tgccccccgg aaacgccgtc ggtgacacga gaaacaccca tgggaagatg
      901 atccccatcg tgaggatgtt gtacacaaat gccgagtagg gcgacatcac tcgcaccagg
      961 ccggaagcct tgcgggtaaa gacctcggca gtcgcagcca tcgtagccat attacgttcc
     1021 tccttttgaa tgatgcaacg cggaccgcag gaatttagcg tatggactgc tcgatagaac
     1081 cctcctcgct cctccgcttt acccggaggt cagctgatgc cccgtgacct ctccatcggc
     1141 aagcgacacc agaaccccgc gcaggatccc ctccccgtac tcgctgcccg ggttgacgca
     1201 cagcgtccga ccgatgttga ttactcctgg agattcgtgg atgtggccgt gtagtcctag
     1261 gaccggttga tgggactcaa tggcccggcg caccgaagcg ctgccggctc cgaacaaaac
     1321 tacctggccg cccttgacga tttgggtcgg cggatcggtg ctccagtcca gcatgggaca
     1381 ggtatccagg ctggaatcaa ccggtgggtc gtgaaagttg aagatgcagt ggctcaggtc
     1441 tggtgactgc cggactgcgg cctcgatcat ttcgcccagt tggtcatccg agacctcccg
     1501 cggcgtgtgc cagggagtag gggtgctgta gcccacgctg atcagggtgt ggtcgtcgtc
     1561 gaggttgacc agcttaccct cgcatccgaa gatagcctgg gtcccgccgc ggtagagcac
     1621 gtcgagcact tcggtatagt cgtcattacc gccggtcaca aagcagcgca ctccggagcc
     1681 ggccagccgg gtctctgcca gttcgaccca ggcctcgagc cgctggcgag ccagttcctt
     1741 gaagagcgcc tccaccgcag ccggttcgga ctggatgcgg cggaactccg cctcatccat
     1801 caccttactg tagaacccca gcgtccccag acgggagcgg agctgctcaa ggccagactc
     1861 gccttcgatg gtctcggtgc gcccttgcag ggtggcgcgg tagcggccgt tggcttcctg
     1921 tatgatcgga atggccagct tgccaagaat atcgccaccc atcaccagca cggcagcctc
     1981 gtagaacttg gcggaattga tgaatttgcg aaaggtacgg tcggagccgt ggacgtcggt
     2041 ggcgaagaag agccgggtgg tcttgcgggc aggcttgcga gggaacaggg acatgccgtt
     2101 tctacttcag ggcgcagcct gctcctgtcc gtccggccgc tcggccgccg cgatccgaac
     2161 ctcgatcgag cgggcccgca aagccgttcg ccattcagga gcgagcccct cgtcggtcac
     2221 cagggtactg acctttgaga gcggggccac cgcaaagttg gacactaccc cccacttgct
     2281 gtgatcggcg acgatgatca ccggaccctg ggtccgctcg atcatcaagc cggcgacttc
     2341 ggcctcggtg ttgatcgggg tggtgcaccc gtgatgcagg ctgatgccat cgacgccgat
     2401 gaatgcctta tcggcaaaga cctggccgat taggccggct gcgaagcggc cggataccga
     2461 attggagcgg ggccgaaagg aaccacccac caagatgagc tcgaacccag gctcacgggc
     2521 ttccagggag gcggccaggt tgttggtaac gaccgtgagg tcggtcagtc cgcgggcgcc
     2581 cagctggcgt aagacttcgg cagtcgtggt gccactgttc acgaagatcg tgtcaccggg
     2641 ctccgccagc gcggcggcgg tcgctccgat ccgccgtttt tcatctgggt gggcgagggc
     2701 actggaagcg taggcgggct cgatgggcag ccgctgggtc agcagcgcgc cgccatgggt
     2761 ccgctcgacc tcgccctccc gctccaacca gaccagatcg cggcggactg tcgcttccga
     2821 aactcccagg atttcgctca gggttgcgct gcgcacgatc tgatggcgct gtaggaactc
     2881 tcgaattcgg tggcgccgct gtgccgggat gagcggctgg ctaacctgca ttcgaccctc
     2941 tagtgatgga aattgagcgg tcttgtgcgg atggggcgcc gtgattatga tcgattctga
     3001 ctagttctgc aagactcaag gcttcggtta caatcgcgcc cgggcagccg agaaacacac
     3061 ccagcatgag ttcctcagca atcgggatgg gcaaccaagg ccaggaagag cggcagcgct
     3121 tcgaggccga ggtgcgccgc attgttgagg ccagcgaagg cggcggggtc acgctgcgcc
     3181 ttttgggttc gctggccttc cacgctcact gtccccagta cggttacctg caggccgccc
     3241 taggccgtgc ctacactgac ctcgactttg gtggctacgt tcgccaggcg aagggtgttg
     3301 gccaaatcct gaccgcgctt ggctaccagg aggaccggga ggtcttcgtg gtcacggaag
     3361 gtggtcgggc catcttcgag caccctggca atggcctgca cgtcgacgtc ttctacgaga
     3421 agcttgattt ctgccacatc attccctggc aggcccgtct cgagctggac tcaccaacct
     3481 tgcccttggc cgagctgttg ctcgagaaga tgcagatcgt cgagatcaat gagaaggatg
     3541 tgatcgacac gatcatgctg ctgctggaac acccgcttgg agtcggggac gcggagacga
     3601 tcaatttgga tcggatcgcg acgttgtgtg ggggggactg ggggctctgg agaacgactt
     3661 cgatgaacct gggtaaggtg cgcaccctcg cc
//
LOCUS       DATDPQ010000347         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_714025, whole genome shotgun sequence.
ACCESSION   DATDPQ010000347 DATDPQ010000000
VERSION     DATDPQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714025"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VI520_07815"
     CDS             <1..1127
                     /locus_tag="VI520_07815"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HLF37825.1"
                     /translation="VGVARRLETCLRQVDTVARLGGDEFVVLLEDIENVGHATATAER
                     IQEQLEENFRLDDNDVFTSASIGIAVSKTGQDRAPDILRDADIAMYRAKSMGKARYAL
                     FDETMHGRAVELLQLETDLRKAVDREEFLLHYQPIVSLLSGEIVGFEALVRWMHPERG
                     LIFPAAFVPLAEETGLSIPMGEWVLREACRQLQTWRAALPSERSLWVSVNLSSRQLLQ
                     PGLIRFVESCLEEAKSDPATLHLEVTEGVIMAEPEAATAMLVGLRELGVGLPIDDFGT
                     GYSSLAYLHRFPITSLKIDRSFVNRVASGGEDEVIVRTIVSLAHNLGMDVIAEGVETA
                     EQLERLTALGCEFGQGFYFSKPVPADEASQLLQLPVAVLA"
     gene            1184..2266
                     /locus_tag="VI520_07820"
     CDS             1184..2266
                     /locus_tag="VI520_07820"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HLF37826.1"
                     /translation="MQRIGILTGGGDAPGLNAVIRAVVKSADQHGGYEVLGFKDGFEG
                     CLDPEGVVELSLDAVHGILPRGGTILGAANRGNPFARKVVRDGKEVMEDVSDQVVRRI
                     EQLELESLIVVGGDGTLHIALELFRKGMPIIGIPKTIDNDIEGTDVTFGFDTAVNTAT
                     EALDRLHTTAEAHHRVIVLELMGRDAGFIALQAGIAGGADVILIPEIPFDFKGICETI
                     QGRASRGRHFSTVAVAEGARPKGGEQVFRRAGDELATARLGGIGQTVAEYVEGVCGME
                     SRVVVLGHLQRGGSPTAFDRWLATRFGAAAFRLAVQQAHGRMVALRGSNIVDLPLDEA
                     LAVPKRVDLSGDGIQTARTLGLCLGD"
     gene            2356..2781
                     /locus_tag="VI520_07825"
     CDS             2356..2781
                     /locus_tag="VI520_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37827.1"
                     /translation="MGKVVSGVSIASEPLRVFAIGFLLAAAFFLVEAGAAEIVLARRS
                     DCVEQLGRMRLAPNPEQACMSEFQFFLARALSRGVFVSADPESSGAIAWPLMAGLYGL
                     LGGALARMKPTRGIGVYLGVHLALLMVLMSLDFISQFIV"
BASE COUNT          539 a          803 c         1005 g          560 t
ORIGIN      
        1 tagttggagt ggcccgccgg ctggagacct gtttgcggca ggtggatacc gtggcgcgcc
       61 tgggtggcga cgaattcgtg gtcttgctgg aggacatcga aaacgtcggc cacgccaccg
      121 cgactgcgga gcgaatccag gagcagctgg aggaaaactt ccggttggat gacaacgatg
      181 tcttcaccag tgccagcatc gggatcgcgg tgagcaagac cgggcaggat cgggcgccgg
      241 acattttgcg ggatgccgac attgcgatgt accgggccaa gtccatgggc aaggcacgct
      301 atgccctctt tgacgagacg atgcacggtc gagcggtcga attgctccag ctggaaaccg
      361 acctgcgcaa agcagtggat cgagaggagt ttctgctgca ctaccagccg atcgtctcgc
      421 tgctctccgg cgagatcgtc ggattcgaag cgctggtccg ctggatgcat ccggagcggg
      481 ggctgatctt cccggctgcc tttgtgccct tggcggagga gaccgggctc tccattccaa
      541 tgggtgagtg ggtgctgcgc gaggcctgtc gccaattgca gacctggcga gctgcccttc
      601 cgagcgaacg cagcctgtgg gtcagtgtca atctctccag ccgtcagctg ctgcagcccg
      661 gcctgatcag gtttgttgag agctgcctgg aggaagccaa atccgacccg gccactctgc
      721 atctggaagt aaccgaaggc gtcatcatgg ccgagccgga agcagccacg gccatgctgg
      781 ttggcttgcg ggagctcggg gttgggctgc cgatcgatga ctttgggacc ggatactcct
      841 cgttggccta cctgcaccgg ttcccgatca cttcgctcaa gatcgaccgc agctttgtca
      901 atcgggtcgc ctcgggaggg gaggacgagg tgatcgtgcg aacgatcgtg agtctcgccc
      961 acaatctcgg aatggatgtg atcgccgagg gagtagagac cgcagagcag cttgagcgac
     1021 tcaccgcact gggttgcgaa ttcggacaag ggttttattt ctcgaaaccg gttccagccg
     1081 atgaagcttc gcaattgctt cagcttccgg tcgcggtctt ggcctgaccg ggtagaatcg
     1141 ccccactcca gagactcgga ttgtcagctc aatgaggtga agcatgcagc gaatcgggat
     1201 tctcaccggt gggggcgacg cgcctggact caatgccgtg attcgggcgg tagtgaagtc
     1261 ggccgatcag catggcggct atgaagtgct cggatttaaa gacgggttcg aaggctgcct
     1321 ggatccagag ggtgtggtgg agctcagtct ggacgcggtc catgggattc tgccccgcgg
     1381 cggaacgatc ttgggagcgg ccaaccgggg caacccgttc gccaggaaag tcgtccggga
     1441 tggcaaggaa gtgatggagg atgtgtcgga ccaggtcgtg cgtcgaatcg agcagcttga
     1501 attggagagt ctgattgtgg taggcggaga tggaacgctg cacatcgcgc tcgagctctt
     1561 ccgtaagggc atgcccatta tcgggatccc gaagacgatc gacaacgaca ttgaggggac
     1621 cgacgtgact ttcggctttg acacggcggt caacacggcg accgaagcgc tggaccgcct
     1681 gcacacgacg gcggaggccc accaccgagt gatcgtgctg gaactgatgg ggcgagacgc
     1741 cggcttcatc gccctgcagg cgggcatcgc aggtggagcg gacgtgatcc ttatcccaga
     1801 aatcccattt gatttcaaag ggatatgtga gacgatccag ggccgcgcca gccggggcag
     1861 gcatttcagc accgtggcgg tggccgaggg cgcccggccg aaaggcggag aacaggtttt
     1921 ccgaagggcg ggcgacgagc tggcgacggc ccgcctgggc ggcatcggcc agacggtcgc
     1981 ggaatatgtg gaaggggtgt gcggtatgga gtccagagtc gtggtgttgg gacatctgca
     2041 gcggggcggt tcgccaaccg cattcgaccg ctggctggcg acccgctttg gcgccgccgc
     2101 ctttcggctg gccgtacagc aggctcacgg ccggatggtt gcattgagag ggtcgaacat
     2161 cgtagacctg ccgctggacg aagcactggc agtcccgaag cgggtggatc ttagtggcga
     2221 cgggatccaa accgcccgga cattggggct ttgcctgggt gactgaccat gcatgaacaa
     2281 ggggatcagg gtaccgatac agaaatgcgc atagccgaat caccggccaa cagagatcag
     2341 cgaacaggcg atcctgtggg caaggtagtg agtggggtca gcatagcctc cgaaccgctg
     2401 cgcgtcttcg caatcggatt cctgctggcc gcggcgttct ttctcgtcga ggcgggcgcg
     2461 gccgagatcg tgctggcccg caggtcggac tgtgtcgagc agctcggtcg aatgcggctt
     2521 gctccaaacc ctgagcaggc ctgcatgtca gaatttcagt ttttcctggc tcgagcgtta
     2581 tctcgggggg tgttcgtctc ggcagacccc gagtcatccg gggcgatcgc ctggccgctg
     2641 atggccgggt tgtacggcct gttgggcggg gcgttggccc ggatgaagcc gacccgcggg
     2701 atcggagtct acctcggcgt ccacctggcc ctgctgatgg tgctgatgtc gctggatttc
     2761 atctctcagt tcatcgtcta gaacaaagct gattcgggtc tgggaaaagg aaggagccgc
     2821 ctcatcggcg gctccgcctt ttgcgctgcg gcacttcctc atcctagacg gaggaggggt
     2881 gccgcagcgc gacgttcgtg agagcta
//
LOCUS       DATDPQ010000348         5287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2550894, whole genome shotgun sequence.
ACCESSION   DATDPQ010000348 DATDPQ010000000
VERSION     DATDPQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550894"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VI520_07830"
     CDS             complement(<1..259)
                     /locus_tag="VI520_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37828.1"
                     /translation="MAEKQAVRAPVTPVTAFDWTQVARLMLRSRALDELEEAELAPSG
                     EVPYQFSAKGHEMAQVLLALSLDRPHDAAGVYYRSRPFMLSV"
     gene            326..1591
                     /locus_tag="VI520_07835"
     CDS             326..1591
                     /locus_tag="VI520_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HLF37829.1"
                     /translation="MATEPSSRLTADRLALVYQLSKTFNSSLNLEDVLNSVMDEVISA
                     THAERGFVVLRADDGQLEFSAARGVDQATIGQPEFQISRGVMEQVARDGEGVLTSDAQ
                     QDERFMSRQSVMSLGLRSILCAPLRVKEKVLGAIYVDNRLHAGIFMPADLELLIAIAA
                     SAATAIENARLYQVAVDKGRMERELSMARRVQTGLIPETIPELAGWEFAASWLPAREV
                     AGDFYDFIPRDGRDLGLVIADVTDKGMGAALFMALTRSMVRASLEGEVSAAEAISKAN
                     RLICANSTTAMPVTLFYGRVTPSGGELIYVNAGHNPPLLYRSASGSFEELGRTGLVLG
                     VDASITLDQHSTTLEPGDCLVLYTDGVTDALNAAEQPFGEQRLRSTIEASASGSAQAI
                     ALALLQALQEFIGGADPFDDITLLVAKRS"
     gene            1588..5139
                     /locus_tag="VI520_07840"
     CDS             1588..5139
                     /locus_tag="VI520_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLF37830.1"
                     /translation="MTDLAVGTMLNDRYQLEGELGRGGMGIVYRAHDTLLDRDVAVKM
                     VSSLNLGTEGRARLLGEAKAIAKLNHPHIVAVYDAGEIEKSPFVVMELIAGQSLHDNP
                     PKDFETIVRVARDISRALEHAHSQGIVHRDLKPENVVLLPDGTAKLMDFGIARSVTSR
                     MTSEGNIIGTVFYLAPELALGQEFDGRADLYALGVMLYELTTGSLPFSHSDPLAVISQ
                     HLHASVVPPRAKRPEVPPLLDELILRLMSKRPEDRPASAGEVLEALQRSDLLDPEARA
                     GQELRVIDRIARGRFIGREQELNEARELWGKAAGGQGQTLLISGEPGIGKTRLMRELA
                     THVEVTGGRTLVGTCYAEGGAPYAPFAQIVRRTLGKGAQNGHPLPEFVLADLLSLAPD
                     LKPYYPELSPNPPLEPQAEQQRLFENVVAFCGTLSEESPVLLVLDDAHWADSGSLSML
                     RHLARRTRRNRLLLMATYREVELNEARPFHEVMLDLNRERLATRLKLARLTREETQAL
                     LAAIFEEEITSDFLDGIYRETEGNPFFIEEVCKTLVEEGKLYFENGRWHRPSMDELEI
                     PQSVRVAIQSRITTMPEQFQETLRLAAILGREFDYDTLAKASEVSEDALIEALEAAEH
                     AQLIEEVSGKGGVTFSFLHALIPATLEESVHTLRRRKLHRQAAAAIESLRPEDHEALA
                     YHYGEAGDEAKALNYYTQAGEAAARAYANREAESHFRAALNLVQTDAQRAPLLAQLGL
                     VLSNQAQFEGAIETWKSAIGIFQSMGEKARVAWCYARAARAAWEAGDFPRGLTLSKEG
                     MKAVEGAAESAELADLLHEAARACYFNGIEAEGVPLSRRALEMAERQGATKIQAESLI
                     TWALFDKVPTDEAIAALERAVELSKADLLPEQEARAHNNLALWVGVYRGQIGSARQHL
                     RRAQELARKTGQVASELFYTSNEVSWAITQGELPYAEVEIVRLSRLSEEASLSGTAAL
                     VFRRLEGDLWDARGQLEAAIERYREILAEARAGGLLQDVWATSTSLGETLIEIGKLQE
                     AEAALLEAITIGDKIGIPGWARLLMSRLHSKGGDRDQSRHFLQEAELKMAQRPAALET
                     ANTALARAELDRLEGRWEEAWSHFESHLDQMAAMGLRLGHAQGMRGWAEAYLARGEPD
                     DPARARELLAKARAEFENMGAAGYVDKIDAQLKEIGN"
BASE COUNT         1014 a         1678 c         1705 g          890 t
ORIGIN      
        1 cgacgcttag catgaacggt cgggagcggt agtacacgcc cgccgcgtcg tggggacgat
       61 ccaggctgag cgccagcagg acctgggcca tctcatggcc cttagccgag aattggtacg
      121 gtacctctcc gctgggcgcc agttcggctt cctccagctc gtcgagcgcc cgcgaacgca
      181 gcatcagccg ggccacttgg gtccaatcga aagcggtaac cggtgtgacc ggagcccgga
      241 ctgcctgctt ctcggccatc cttgcgatta tactgacatc agcccatgat gcttgcaact
      301 caagcatcat tttcaactac cgagtatggc gaccgaaccc tcctcaagac tgaccgctga
      361 ccggttggcg ctggtctatc agctctcgaa gaccttcaac tcctccctca acctggaaga
      421 cgtgctcaat tcggtaatgg acgaggtgat ctccgccacc catgctgagc gcggcttcgt
      481 ggtgctgcgg gcggacgacg ggcagctgga gtttagcgcc gcccgagggg tggatcaggc
      541 gaccatcggt caaccggagt ttcagatttc gcggggcgtg atggagcagg ttgcccggga
      601 tggcgaaggc gtactcacca gcgacgccca acaagatgag cggttcatgt cacgccaaag
      661 cgtgatgagt ctcggtctgc ggtcgatcct atgtgcgccg ctgcgggtca aagaaaaggt
      721 gctgggggct atttacgtcg acaaccggct gcatgcggga atcttcatgc ccgccgattt
      781 ggaactgctc attgcgattg ccgccagcgc ggctaccgcc atcgagaatg cccggctata
      841 ccaggtggcg gtggacaagg gacgcatgga gcgtgagctg tcgatggccc gccgggtgca
      901 gaccggcctc attccggaga cgatcccgga gcttgccggc tgggagttcg ccgccagctg
      961 gctgccggct cgggaggtcg ctggagactt ctacgacttc atcccccgcg acggccggga
     1021 tctgggattg gtcatcgccg atgtcaccga caaggggatg ggcgccgcgc tgttcatggc
     1081 gctgactcgc agcatggtgc gggccagcct ggagggagag gtgagcgcgg ccgaagctat
     1141 ctcgaaggcc aatcggctga tctgcgccaa ttccacgacc gccatgccgg tcaccctctt
     1201 ctatggaaga gtgacgcctt ccggcggcga actaatctat gtgaatgccg gccacaaccc
     1261 gccgctgctg tatcgctccg cttcgggctc attcgaagaa ttgggccgga ccggcctggt
     1321 gctgggagtg gatgcctcaa tcaccctcga tcagcactcc acaactctcg aaccgggcga
     1381 ctgcctcgtg ctgtacaccg acggagtaac cgacgccttg aatgcggccg agcagccttt
     1441 tggagagcag cgcctgcgca gcacgatcga ggcgagcgct tccggttcgg cccaagcgat
     1501 tgcgctggcc ctgctccagg ccctgcagga attcatcggc ggggccgatc ccttcgacga
     1561 catcaccctg ctggtcgcca aacgctcatg accgacctgg ccgtcgggac catgctcaac
     1621 gatcgctacc agctggaagg agagctcgga cggggcggca tgggcatcgt gtaccgggcc
     1681 cacgatacgc tgctcgaccg agacgtggca gtcaagatgg tctccagctt gaatctcggc
     1741 accgaggggc gggcccgcct cttaggagag gcgaaggcga tcgccaagct caatcatccc
     1801 cacatagtcg ccgtctatga cgccggagag atcgagaagt cgcccttcgt cgtgatggag
     1861 ctgatcgccg gccagagcct gcacgacaat cctccgaagg atttcgagac cattgtcagg
     1921 gtcgcccgcg acatttcccg ggccctcgag cacgcccact cgcaggggat cgtgcatcgc
     1981 gatctcaaac ccgaaaacgt ggtcctgctg ccggatggga ctgccaaact gatggacttc
     2041 gggatcgccc gctcagtgac ctctcggatg acgtccgagg ggaacatcat cgggacggtg
     2101 ttctacctgg cgccggagct ggcactgggc caggagttcg acggacgggc cgacctgtat
     2161 gccctcgggg tgatgctgta cgagttaacc accggcagcc tgcccttctc ccacagcgat
     2221 ccattggcag tgatctcaca gcatctgcac gcctcggtcg ttccgccgcg ggctaagcgc
     2281 cccgaggtcc caccactgct cgatgagctc atcctgcgcc tgatgagcaa gcggccggag
     2341 gaccgtccgg cctccgccgg cgaagtgctc gaggcccttc agcgctccga cttgctcgac
     2401 ccggaggcca gggcggggca agagctgcgg gtgatcgacc ggatcgcccg cggccgcttt
     2461 atcggccgcg agcaagagct aaatgaagcg cgcgagctgt ggggcaaggc ggccgggggt
     2521 caagggcaga ccctgctgat cagcggcgaa ccaggcatcg gcaagacccg gctgatgcgc
     2581 gaattggcga cccatgtcga ggtcaccggc ggccgaaccc tggtcgggac ctgttacgcc
     2641 gagggcggcg ctccctacgc gccatttgcc cagatcgttc gacggaccct cggcaagggg
     2701 gcgcagaacg gccatccgct gccggagttt gtgctggccg acttgctcag cctcgccccg
     2761 gacctcaaac cctactaccc cgagttgtct cccaatccgc cattggaacc gcaggccgag
     2821 cagcagcggc tcttcgagaa cgtcgtggcg ttttgcggca cgttgagcga ggaaagcccg
     2881 gtgctgctgg tgctggatga tgcccactgg gccgacagcg gttcgctctc gatgctgcgt
     2941 cacctggccc gccggactcg ccgcaaccgg ctgctgctga tggccaccta ccgggaggtc
     3001 gagctgaacg aagcccggcc cttccacgag gtgatgctcg atctaaatcg ggagcggctg
     3061 gcgacccggt tgaagctcgc tcgtctgacc cgcgaagaga cccaagcgct gctggccgcc
     3121 attttcgagg aagaaatcac ctcggacttc ttggacggaa tctatcgcga gaccgagggc
     3181 aaccccttct tcatagaaga agtctgcaag actttggtgg aggaaggcaa actgtacttc
     3241 gagaacggcc gctggcaccg gcccagcatg gatgagctcg agatcccgca gagcgtgcgg
     3301 gtggccattc aatcccggat cacgaccatg cctgaacagt ttcaagaaac gctgcgcctg
     3361 gccgccatcc tgggtcggga gttcgattac gacaccctgg cgaaggccag cgaggtcagc
     3421 gaagacgcgt tgatcgaagc cctggaggcg gccgagcacg cccaactcat cgaagaggtc
     3481 agcggcaaag gcggcgtgac tttctccttc ctgcacgcct tgatcccagc cacgctcgag
     3541 gaaagcgtac acactttgcg ccgccgcaag ttgcatcgcc aggccgcggc ggccatagag
     3601 agcctgcggc ccgaagatca cgaagcactc gcctaccatt acggtgaagc cggcgacgag
     3661 gccaaggctc ttaactacta cactcaggcc ggcgaggccg ccgcccgggc ctatgccaat
     3721 cgggaggccg agagccactt ccgggccgcg ctgaacctcg tccaaaccga cgcccagcgg
     3781 gcgccgctgc tagcccagct cggcctcgtg ctgtccaatc aggcgcagtt cgaaggtgcg
     3841 atcgagacct ggaagtcggc cattggcatc ttccaatcga tgggtgagaa ggcgcgggtg
     3901 gcctggtgct acgcccgtgc cgcccgcgcc gcatgggagg ccggcgactt cccgcgaggc
     3961 ctcactctct ccaaggaagg aatgaaggct gttgagggtg cagctgagag cgcagaattg
     4021 gccgacttgt tgcatgaagc agcccgagcc tgttatttca atggaataga agcggagggc
     4081 gtgcctctct cgcggcgggc cctcgaaatg gccgagcgac agggggcaac aaagatccag
     4141 gccgagtcgc tgatcacctg ggccctcttc gacaaggtac cgaccgatga agccatcgct
     4201 gccctcgagc gagcggtcga gctttccaaa gcggacctgc tcccggagca agaagcgcgg
     4261 gcgcacaaca atctcgccct ctgggtaggc gtttaccggg gccagatcgg ttccgcccgc
     4321 cagcaccttc gacgggcaca ggagctggcc cgcaagaccg gccaggttgc gagcgaactc
     4381 ttctacacgt ctaatgaagt gagctgggcg atcacgcagg gcgagctgcc atacgcagag
     4441 gtagaaatcg tccggttgag cagactctcc gaggaggctt cattgtccgg cactgctgcg
     4501 ctcgtcttcc gcaggcttga aggtgacctg tgggacgcac gcggtcagct agaggctgcc
     4561 atcgagcgat atcgcgagat cctggccgag gcccgcgctg gcgggctact ccaagatgtt
     4621 tgggccacca gcacctcgct cggagaaacg ctgatcgaaa tcggcaaact tcaggaggca
     4681 gaagccgccc tacttgaagc gatcacgatc ggcgacaaga tcgggatacc tggctgggcc
     4741 cggctgctga tgtcgcggct gcacagtaag gggggtgacc gtgatcagtc ccgacacttc
     4801 ctccaagagg ctgagctgaa gatggcccag cgacccgctg cgctggaaac cgcgaatacc
     4861 gccctggcca gggccgagct cgaccgattg gaaggccgct gggaagaggc ctggagccac
     4921 tttgagagcc acctcgacca aatggcggcc atgggattgc gcttggggca cgcccagggg
     4981 atgcgcggtt gggcagaggc ctatctcgcc cgcggcgagc cggacgaccc ggcgcgggcg
     5041 cgggagctgc tggccaaggc ccgggcggaa ttcgagaaca tgggggccgc tggctatgtc
     5101 gacaagattg acgcccaatt gaaagagatc ggaaactagc gccatcggtc cgtatgatgc
     5161 ggatcgacca gctccagcct cgctaagtga tctccgtacc gatccccaaa atcctgaatg
     5221 accgctaccg cctaggcgag gagctcggcc gcggcgggat ggggaccgtc caccgggccc
     5281 acgacag
//
LOCUS       DATDPQ010000349         2429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2823564, whole genome shotgun sequence.
ACCESSION   DATDPQ010000349 DATDPQ010000000
VERSION     DATDPQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2823564"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VI520_07845"
     CDS             <1..973
                     /locus_tag="VI520_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HLF37831.1"
                     /translation="LQMESARVQAVKDSGTVRCGVNTGVPGFGFLNEQGEFVGFDIDF
                     CRAIAVAIFNDPNAVEFRPLTAAERFTALQTNEIDVLIRNTTWTLTRDVQLGTDFGTT
                     TFYDGQGMMVRRDSGFASLGDMAGATVCVLTGTTTEGNLADAFNERGLSYEPLVFATS
                     DETRVAYGEGRCDGLTSDKSQLNGLRTALEVPADHIILPEAMSKEPLGPVWAQGDQQW
                     GDVVRWVVNAIILAEEKGITSSNVDGIVASDPQDIEVRRLLGIEGEFGAMLGLAPDFV
                     VNVIREVGNYGEIYDRHLGPNGIDIPREGSLNELWMDGGLLYALPWK"
     gene            1059..2243
                     /locus_tag="VI520_07850"
     CDS             1059..2243
                     /locus_tag="VI520_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLF37832.1"
                     /translation="MLRSFQGSLQSLSRFSRDIRFLQAVAQAAFVVLVVVIGNWLLGN
                     LLDALDTRGLDLSFGFLGRTGGFEVADEALHLDRSDSFLDAYFVGLVNTLQVVLVGLI
                     LATALGIFTGIALLSSNWLLRSISQAYVEIIRNTPLLVQLFFLYSGILLKLPELADRL
                     TFGPFILSNRGFFTPRAIPQESSRPWLLLAALGALLAVWTYRWQIDRRLREGKETRPI
                     LWAAAPLFGLPLLGWLVLPGQPFTAEIPELEGLRIVGGSRLSPEFAAILLGLVIYTAA
                     FIAEIVRAGILSVPHGLREASLAGGLSRTQTLRLVVLPVALRVIIPPTTNQYLNLAKN
                     SSLAIGVGYPDLYSISQTIFNQSGQAVQVIAMMMGTYLALSLMISAGMNFINARLKIR
                     ER"
BASE COUNT          417 a          728 c          736 g          548 t
ORIGIN      
        1 gttacagatg gaatctgccc gcgtacaggc cgtcaaggac agcggtacgg tgcgttgcgg
       61 cgtaaacacc ggcgtcccgg gctttgggtt cctcaatgag cagggcgagt ttgtcggctt
      121 cgatatcgac ttctgccgtg caatcgcggt agccatattc aacgatccga atgcagtgga
      181 gttccgcccg ctgactgcgg ccgagcgttt cacagcgcta cagaccaatg aaatcgatgt
      241 cctgatccgc aacacgacct ggaccctcac ccgcgacgtg cagctcggca ccgatttcgg
      301 caccacgact ttctatgatg gtcagggaat gatggttcgc cgtgatagtg gttttgcctc
      361 gctgggcgac atggccgggg cgacggtatg cgtgttgacc ggtaccacaa ctgagggaaa
      421 cctggccgat gctttcaacg agcggggtct tagctatgaa ccattggtgt tcgcgacctc
      481 ggacgaaacc cgggtcgcct acggcgaagg ccgttgcgat ggcttgacct cggacaagtc
      541 acagctcaat ggtctgcgta ccgcgttgga ggtccctgcc gaccacatca tcctgcccga
      601 ggcgatgtcg aaggaaccgc tagggccggt gtgggcccag ggtgatcagc agtggggtga
      661 cgtcgtccgc tgggtcgtga acgcgatcat cctggccgag gagaagggca tcacctcatc
      721 gaatgtggat gggatcgtgg cttccgaccc gcaggacatt gaggtccgcc gactactcgg
      781 gatcgagggc gagttcgggg cgatgctggg actcgctccg gacttcgtcg tcaatgtcat
      841 ccgcgaggtg ggcaattacg gtgagatcta tgaccgccac cttggcccga acggaatcga
      901 catcccccgg gagggcagcc tgaacgagct gtggatggac ggcggcctgc tctacgcctt
      961 gccttggaag tagttccctt tctcgggcga gtcgactctg aagatgcggc ccggcctacg
     1021 gccgggccgc atcttgttag ccacagtccg ctcattcgat gctgagaagt ttccagggct
     1081 cgcttcagtc tctcagtcgc ttctcgcggg acatccgctt cctgcaagca gtggctcagg
     1141 ccgcctttgt agtgctggtg gtcgtcatcg gcaattggtt gctcggcaat ctcctcgacg
     1201 ccctggacac tcgcgggctc gacttgagct tcggtttcct cggcagaacc ggcgggtttg
     1261 aggtcgccga cgaggcgctc catctcgatc gatccgatag cttcctcgat gcttatttcg
     1321 tcgggctggt gaatacgctg caggtagtgc ttgttggcct gatcctggca actgcgttgg
     1381 ggatattcac cggtatcgcg ctgctatcct ccaattggct gctgcgctca atttcgcagg
     1441 cctatgtcga gatcatccga aataccccgc tcttggtgca gttgttcttt ctatactccg
     1501 gcatcctcct caagcttcct gaacttgccg accgtctcac tttcggtcca ttcatcctga
     1561 gcaatcgcgg cttcttcacc ccgcgggcca tcccacagga atcatcacgg ccatggctgc
     1621 tgcttgccgc tctcggcgcg ttgctggccg tgtggaccta tcgatggcag atcgatcgca
     1681 ggctgcgcga gggaaaagaa actcggccga ttctgtgggc cgcagcccct ttgttcggac
     1741 tcccactgct tgggtggctg gtactccccg gccagccgtt caccgctgag attcccgagc
     1801 tcgagggcct gcggattgtc ggtggatcgc gcctcagccc cgaatttgcc gccattctgc
     1861 tcggtctggt gatctatacg gcagcattca tcgccgaaat cgtccgggcc gggatcttgt
     1921 cggttcccca cggtctgcgg gaggcctcgc tggccggcgg attgagccgt acccaaacac
     1981 tgcggctggt cgtccttcca gtagcgctgc gtgtcatcat tccacccacc acgaaccagt
     2041 acctgaatct ggcgaagaat tccagcctgg ccatcggcgt cggttacccc gatttgtatt
     2101 ccatttcgca gaccatcttc aatcaatccg gccaggcggt gcaggtcatc gcaatgatga
     2161 tgggtaccta cctggctctc agtttgatga tttccgcagg tatgaacttc atcaatgctc
     2221 gcctgaagat cagggaaagg tagtccacgg ggatgggcga gcttgcagct ctccggccga
     2281 tcgagcagcc gcctcaaacg agggtggggg ttctcggctg gctgcgcaag aacctcttca
     2341 gcagctggcc tagcgggctg gttacgattg gggttggagc cgcgctctac gccatcggtc
     2401 gctcgctctt ggcctgggtg ttcgcagaa
//
LOCUS       DATDPQ010000350         3658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2309326, whole genome shotgun sequence.
ACCESSION   DATDPQ010000350 DATDPQ010000000
VERSION     DATDPQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309326"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VI520_07855"
     CDS             complement(<1..405)
                     /locus_tag="VI520_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37833.1"
                     /translation="MKPTVWRIVLAIAAVISIASLAFSAGLATGLVIPRLSGPSEAPP
                     LELNPPQINQPEPGPQLPASRDELMAPFWEAWEILHDEYVDQPLDDGSLVQGAIRGMV
                     DSLGDPHSSYMDPDEYLQANLPLDGEYEGIGAW"
     gene            complement(498..1304)
                     /locus_tag="VI520_07860"
     CDS             complement(498..1304)
                     /locus_tag="VI520_07860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HLF37834.1"
                     /translation="MDKRISLQESVSLLPAGEVSLGLGGFTLYRRPMAFTLALLAALN
                     SSDSRPTLTLLTFTAGLESDLLVGAGIISRVRTCYFGLEAFGLAPRFTQAAGEGTLEV
                     VEETEASLALGLRAALSGVGFLPSHAWQGTDLLRLRPDVKEVADPYSGEILTAFPPIR
                     CDLAVIHALRADHDGNADIGQNWGVDRELALVADKVIVTAEQVVPRLETAQVFGHAVT
                     AVVEAPGGAWPTSCYPNYRLDGEAVLAYIDSGGADPRLIREWAARHQVTL"
     gene            complement(1343..1576)
                     /locus_tag="VI520_07865"
     CDS             complement(1343..1576)
                     /locus_tag="VI520_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37835.1"
                     /translation="MADMSPATVVCLALGLVVAINGLLILSLTRGGLQRQVELMRTAA
                     RAAQSPWKTEDEALSELHRRVAQLTEDQTDDPG"
     gene            1618..3396
                     /gene="mutL"
                     /locus_tag="VI520_07870"
     CDS             1618..3396
                     /gene="mutL"
                     /locus_tag="VI520_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLF37836.1"
                     /translation="MTVAEAGQPAVGAVAGEPRFIIGPSQMPIRQLSKEVSSAIAAGE
                     VVERPASVIKELLENAIDAGARSIEVVIEGGGKRLVMVSDDGEGIRANELSLAVGRYS
                     TSKLESVEDLRSIRSLGFRGEALASIGAVSRMEIVTRARGAAAGSRLVVEGGRIGEPE
                     PVGASPGTLVRVQDLFFNVPARRKFLKSEPTELGWIARLVSRYAMAYPDRRIRFEGEG
                     RERLHTGGSGDRREALAAVYGLQDAQAMIPLMEPAMDGFGFALSGFIGPPPIHRSNRR
                     EITIFVNGRWIQDVGLGSAVTQAYHGLLMVGKYPVAALFLQIAPDQVDVNVHPAKAEV
                     RFADPQLVFSGVQRAVRASLLGQVPVHPLSLGPSGPTPGWTGSALHADRAAAPERVIQ
                     LPTEAAGAVPILRSVGQVGATYLVAEGPDGVYLIDQHAAHERVLYERLMKQADSQSPE
                     SQSLLEAITVEFGPSEINTLELNLSALGALGFRVEPFGPQAYRVRAVPAVLSGIAPDR
                     ALRAVVEDFEEDETPLGEETEARLAARVCKRAAIKAGQILSLEEQRELLRDLERCQAP
                     RTCPHGRPTMIHLSVAALERQFGRRG"
     gene            complement(3397..>3658)
                     /locus_tag="VI520_07875"
     CDS             complement(3397..>3658)
                     /locus_tag="VI520_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37837.1"
                     /translation="EVLMQVRDTGRGISPENQTRLFEKFYRVSDSDDEVQGTGLGLAI
                     AKRVAESHGGGITVESALGKGSSFTVHLPSGPMTEVTTRPRF"
BASE COUNT          664 a         1105 c         1210 g          679 t
ORIGIN      
        1 ccaggcgcca atgccttcgt actcgccgtc caggggaagg ttggcctgca ggtattcatc
       61 tggatccatg taggaggaat gcggatcgcc cagcgaatcg accatgcctc ggatggctcc
      121 ctgcaccagc gagccatcgt ctagcggctg gtcgacatac tcgtcatgca ggatctccca
      181 ggcctcccag aacggggcca tgagttcgtc gcgggaggca ggaagctgtg gacccggttc
      241 tggctggttg atttgcggcg gattgagctc gagcggtggc gcctccgacg ggcccgacag
      301 ccgcgggatc accagtccgg tggccaggcc agcggagaag gcaaggctcg cgatgctgat
      361 cactgcggcg atggcaagca caattcgcca gaccgtgggt ttcataggta agctcctctt
      421 gggtggcaac ttatcacgca tacctaagag cggaatgaat gtagacgcaa tgcggcaatc
      481 gggctagccg aggggggcta cagggttacc tggtggcgtg ccgcccactc gcggatcagc
      541 ctcggatcgg ccccgccaga atcgatatag gcgagtacgg cctctccatc taggcggtag
      601 tttggataac aggaggtggg ccatgctccc ccaggagcct cgacaaccgc agtgaccgcg
      661 tggccaaata cctgagccgt ctccaagcgg ggaactactt gctcggctgt gacgattact
      721 ttgtccgcga caagcgcaag ttcgcggtcg actccccaat tctggccgat atccgcattc
      781 ccatcgtgat cggcccgcaa tgcgtgaatg accgccagat cgcatcggat cggcgggaat
      841 gccgtcagta tttcgccgct gtaggggtcg gcgacttctt tgacatccgg ccgcaaccgg
      901 agcagatcgg ttccctgcca ggcgtgggag ggaagaaagc cgactccgga gagggcagcc
      961 cgcaagccca atgccaggct ggcctctgtc tcctcaacga cttcgagcgt cccttcgccg
     1021 gcggcctggg tgaaccgggg ggccaggcca aaggcctcca gtccaaaata gcaggtcctg
     1081 actcgggaga tgatccccgc cccgactaac aggtcgctct ccaaaccggc agtgaaggtg
     1141 agcagggtga gggtgggccg cgaatccgat gaattgagag ctgccaggag cgccagggtg
     1201 aaggccatcg gtcggcgata cagcgtgaaa ccaccgagcc ccaggctgac ctctccagcc
     1261 ggcagaagcg acaccgattc ctgcaggcta atccgcttat ccattaggtt gaaggagggg
     1321 agccaccttt cgggtccgag ccctagcccg ggtcgtcggt ctgatcctcg gtgagctgcg
     1381 caactcgccg gtgcagctcg ctaagggctt cgtcctctgt tttccaaggg ctttgagccg
     1441 ctcgagcggc ggtacgcatc aactcaacct ggcgctgcag gccgccgcgg gtcaggctga
     1501 ggatcagcag tccgttgatg gcaacgacca gccctagcgc aaggcaaacg acggtcgcgg
     1561 gactcatgtc ggccactgac caggcggaga tttactcgcg gggtcacggc gcagtatatg
     1621 acggtggccg aggccggtca acccgcagtc ggcgcggtcg cgggtgagcc gcgctttata
     1681 atcggtccga gtcagatgcc gatccgccaa ctctcaaaag aagtctcctc ggcgatcgcg
     1741 gcgggggaag tggtcgagcg accagcctcg gtcatcaagg aactgctgga aaacgccatc
     1801 gatgccggtg cccgctcgat cgaggtcgtg atcgagggcg gcggcaagcg gctggtgatg
     1861 gtttccgacg atggggaggg gattcgagcc aacgagcttt cccttgcggt cggacggtat
     1921 tccacttcca agcttgagtc ggtggaggac ctgaggtcga tccggagcct aggatttcgc
     1981 ggcgaggcgc tggcctcgat cggagcggtc tcccggatgg agatcgtcac ccgggcccgt
     2041 ggagcagccg ccggttcgcg actcgtagtg gaaggcggcc gaatcggcga gccagagccg
     2101 gtcggcgcgt caccgggtac gctggtgcgg gtccaagacc tcttctttaa cgttcctgcc
     2161 cgcagaaaat tcctgaagtc cgagccaact gagctcggct ggatcgcacg actcgtcagc
     2221 cgctacgcga tggcctatcc ggaccggcgg atccgatttg aaggcgaggg gcgggaacgg
     2281 ctgcataccg ggggcagcgg cgatcggcgc gaggcgttgg cggcggtcta cggattgcag
     2341 gatgcccaag cgatgatccc gcttatggaa ccggctatgg atggtttcgg gtttgcgctc
     2401 tccggcttca tcggccctcc accgattcac cgatccaatc gacgtgagat tacgatattc
     2461 gtgaatgggc gctggatcca ggatgtcgga cttggctcgg cggtcaccca ggcctatcac
     2521 ggcctgctca tggtcgggaa gtatccggtg gcggcgctgt tcctccagat cgctccggat
     2581 caggtggacg tcaacgtcca tccggccaag gcggaggtcc gtttcgcaga tccgcaactc
     2641 gttttctcgg gggtgcagcg ggcagttcgg gcctcgctgc ttggccaggt cccggtccac
     2701 ccgctttccc tcggtccgtc tggcccgacc ccgggatgga ccgggtcagc gctccacgcc
     2761 gaccgggccg cggccccgga gagggtgatt caactaccta ctgaagccgc cggcgcggtt
     2821 cccatccttc gatcggtcgg acaagttggg gcgacttatt tggttgccga gggaccggat
     2881 ggggtctacc tgatcgacca gcatgccgcc cacgagcggg tgctgtatga acgcctcatg
     2941 aagcaagccg actcgcagtc gccggagtcc cagtcgctgc tggaagcgat caccgtagag
     3001 tttgggccga gtgaaatcaa tacgctggag ctcaacctga gcgccctcgg tgcgctgggc
     3061 tttcgagtcg aaccattcgg tccccaggcc tatcgagtgc gggcggtgcc tgccgtactt
     3121 tccggaatcg cacccgaccg ggcgctgagg gcagtcgttg aggatttcga ggaggatgaa
     3181 actccgcttg gcgaggaaac ggaagcccgg ctggcagctc gggtgtgcaa gcgagccgcg
     3241 atcaaggcag gtcagattct gtcgttggaa gaacagcgag agttgttgcg agacttggag
     3301 cgctgccagg cacctcgaac ttgcccgcat ggtcgaccga cgatgatcca cctatcggtc
     3361 gcggcgcttg agcggcagtt tggtcggagg ggctgattag aaccgcggac gggttgtcac
     3421 ttcggtcatt ggaccggagg ggaggtggac ggtgaagctg cttcccttcc caagtgcgct
     3481 ctccacggtg atccccccgc cgtggctctc ggccactcgc ttggcaatcg ccagtccgag
     3541 accggttccc tgcacctcat cgtcgctatc cgagacccgg tagaacttct cgaacaatcg
     3601 ggtctggttt tccggcgaaa tgcccctccc ggtgtctcgt acttgcatca agacttcg
//
LOCUS       DATDPQ010000351         5772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1774188, whole genome shotgun sequence.
ACCESSION   DATDPQ010000351 DATDPQ010000000
VERSION     DATDPQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774188"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VI520_07880"
     CDS             complement(<1..562)
                     /locus_tag="VI520_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLF37838.1"
                     /translation="MARLRVGVIGCGTIAQIMHLPYLRQLNDLFEISALCDVSRQVLD
                     AMGEYYGVERRYTDYHELLMLDLDAVLLLTSGSHAPPALAALKSGKHVLVEKPLCFTL
                     AEADQLIEAAEASRRTLMVGYMKRYDPGYRHARSLLPSLGELRYIQINVLHPAEAPYL
                     NVHRLVRAGDVPAETLERLATEKDRLA"
     gene            complement(565..1320)
                     /locus_tag="VI520_07885"
     CDS             complement(565..1320)
                     /locus_tag="VI520_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF37839.1"
                     /translation="MDNSDTPVVYMENIVKRFGTVTALEGVDFTVHPREVAALVGDNG
                     AGKSTLIKILTGVHTPTRGQIYFEGKPVQIRSPQDARALGIETVYQDLALVDLMSITR
                     NFFLGRELVGGLGPIRFLKLRRMNQQTRTSLSDIGIEVRSPTEKVERLSGGERQSIAI
                     GRALHFGAKLLILDEPTSALSVGETRKVLNYTLNAKERGLSVIFITHNIHHVYLVADR
                     YTIIRHGKLVGNYLKSELTENDIADLITGVREA"
     gene            complement(1359..2363)
                     /locus_tag="VI520_07890"
     CDS             complement(1359..2363)
                     /locus_tag="VI520_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37840.1"
                     /translation="MRRAAPWSHPLRRALADSPILGPLLTLIVVFVLCSAFVPNFLTL
                     RSISGIVSAATLTGTIAIGVTLLMIAGEFDLSVGALVAVAGYLYAFNTVDGGSPGIAL
                     ILAITIPAALGALNGLILIVSRIPSFIVTLGTRSIYRGLLWVLSTGALVQTLEDLPIY
                     RLFNGRLDVLNQLAEGANFRTALLWLVGLVIVFQWILVRTDFGSHVFAVGGNPGAAKA
                     QGVNIKLVKLATFALGGALAGFTGVLLFSQFKSVRVTTGAGMELSAIAAAVVGGALLS
                     GGSGSIWGSLIGVLLISTLRTGVVLLDLPFIPADNFEAVVGATIVGATILNKWLRSRG
                     "
     gene            complement(2512..4419)
                     /locus_tag="VI520_07895"
     CDS             complement(2512..4419)
                     /locus_tag="VI520_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLF37841.1"
                     /translation="MKEMRLMFAGLVTLALILAACTPAPATTEVAPPEGVEATAVPQE
                     VAPPPDLTRELEFIMVQHALCAWDAFWCTVEQGISDAAADLGVQVTVLGPDEFDLEQT
                     ASLIDQAVAGQPDGIGLTVTDPVLFKEPIDRALAAGIPVVAYNAGNGPIADGISYMTY
                     IGQDEYQGGYLGGLRMVTAGGTQGVCINQQVGHAGLDKRCNGFVDAFAEKGLEAEVLA
                     ISDDPAESQTVIGDYATANPNTDAFLTLGPNGANPFYAWMESAGLEAGDLVHGTFDLS
                     PEITANIESGVTLFGIDQQPYLQGYSAVVWLFMTNRFGITPALPVSATGPGFVDASNV
                     GSTPDPDRDVTLLMVQHATCSWDAYWCVVENGINLAAEQMGITTTVLAPDSFDLERTA
                     ALIDQAVAASPDGIALTVTDPILFKEPIERALAAGIPVVAYDSGAGPIKDNIAYLTFL
                     GADEYQGGFQSGTRLAGQPGAEVGVCINHQVGHTGLDARCQGFVDSMTEKGLSAEVLA
                     ITNDPAQSQTIISDFFTANPQVNVALTLGPNGATPFYAFLEASGFGPGDIAHGTFDLS
                     AEIVAAIEDGTTLFAVDQQPFLQGYGAVMLLNLLLRHGVTPALPVTPTGPGFVDQSNI
                     DIVKALAGQYR"
     gene            complement(4607..5623)
                     /locus_tag="VI520_07900"
     CDS             complement(4607..5623)
                     /locus_tag="VI520_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37842.1"
                     /translation="MQVADSLGITLRDWRAVLAERSELVVFITVAAVFGFFAVSAEHF
                     LSPFALSNILTFASINGIVVIPVALLMISGEFDLSVGSIFAVASYVFALALIDGVAPI
                     LAMLLALIASGLLGLINGWIVTKSGIPSFITTLGTMLAFRGIARAIGGGVFASYGGPR
                     LAIFDVLNGPIVAVNRLSQPPANFRISVLWFVLLAAVVGFVLLRTRYGNWVFATGGNP
                     RAANAQGVPINRVKLLNFMLTGLLAGAAGVIQFGHRTSVDPLRGDGVELIAVAACVIG
                     GVRLSGGFGTIVGPCIGVLLLQMLEQGLVLMRIPVQVFQAVAGFFLILAVLMNTSLGG
                     SAKE"
BASE COUNT         1170 a         1804 c         1761 g         1037 t
ORIGIN      
        1 ttgccagtcg atccttttca gtcgccagcc gttcaagcgt ttcagccggc acatcgccgg
       61 cccgcaccag gcgatgcacg ttgaggtacg gcgcctccgc gggatgcagg acgttgatct
      121 ggatatagcg cagttcgccc aagctcggca gcaacgaccg ggcatgccgg tagccggggt
      181 cataacgctt catgtagcca accatcagcg tgcgccggct ggcctcggca gcctcgatca
      241 attgatcggc ctccgccagg gtaaagcaca gcggcttctc caccagaaca tgcttgccgc
      301 tcttcaatgc ggccagggcc ggtggggcgt gggagccgga cgtaagcaag agaacggcgt
      361 ccaaatcaag catcagcagc tcgtggtagt cggtgtatcg ccgctcgacc ccgtagtact
      421 ctcccatggc atccaagacc tggcgggaaa catcacacag agcgctaatc tcaaacagat
      481 cattcaattg ccggagatag ggcaggtgca tgatctgggc gatcgttcca caaccgatca
      541 ctcccacccg gagacgagcc atggttaggc ctcgcgcact ccggtgatca agtccgcaat
      601 gtcattctcg gtcagttcgc tcttcaggta attgccgact agcttgccgt ggcggatgat
      661 ggtgtatcgg tccgccacca aatacacatg gtggatgttg tgagtgataa agataaccga
      721 aagccctcgt tccttggcgt tcagcgtgta gttgagaacc ttccgggtct cgcccaccga
      781 gagcgccgag gtgggctcat ccagtatcag aagtttggca ccaaaatgaa gcgcccgacc
      841 gatggcaatc gactgccgct cgcctcccga aagcctctca actttctcgg tcggcgagcg
      901 gacctcaatc ccgatatcgc tcaatgaagt gcgggtctgt tgattcatgc gccgcagctt
      961 gagaaagcgg atcggtccga gtccgcccac tagctcacgg cctagaaaga agttacgagt
     1021 gatgctcatc aggtcaacca aggcgagatc ctggtaaacg gtctcgatcc ctagagcccg
     1081 ggcatcctgg ggtgagcgaa tctggaccgg cttcccttca aagtagattt ggccgcgggt
     1141 cggggtatga acgccggtaa ggatcttgat gagggttgac ttgcctgcgc cattgtcgcc
     1201 taccagcgcg gcaacctccc gcgggtggac cgtgaaatcc accccctcca gggcggtgac
     1261 tgtaccaaaa cgcttgacga tgttttccat gtagaccacg ggtgtgtcac tgttgtccat
     1321 cccggtctgc tcctcgatat gttcgggcta ccgcgaaact aaccccggct gcgcagccat
     1381 ttgttgagaa tagttgcgcc gacaatggtg gctccaacca cggcttcgaa attgtccgcc
     1441 gggatgaaag gcaagtcgag cagtaccacg cccgtccgca atgtgctgat caacaggacc
     1501 ccgatcagcg atccccagat gctgccggag cccccactta acagggcccc cccgaccacc
     1561 gccgcggcaa tggctgacaa ttccatgccg gccccggtcg tgacccgaac gctcttgaac
     1621 tggctgaaga gcaagacccc ggtgaagccg gccagggcgc ccccaagggc gaaggtcgcg
     1681 agtttgacca gctttatgtt cacgccctgg gccttggccg ctcccggatt ccctcccacc
     1741 gcgaacacgt ggctcccgaa gtccgtgcgg accaggatcc actggaaaac gatcaccagc
     1801 ccgacaagcc aaagcagggc cgtgcggaag ttggccccct cagcgagttg attcagcaca
     1861 tccagtcggc cgttgaacag ccggtagatc ggtaggtcct ccaaagtttg gacgagggcg
     1921 ccggtggaga gcacccacag caaacctcga tagatggagc gggtacccaa ggtcacgatg
     1981 aacgacggaa tccggctgac gatcaggatc agcccgttga gggcgcccag cgcagcaggg
     2041 atggtgatcg ccaggataag cgcgatcccc ggtgatccgc cgtcgacggt attgaaggca
     2101 tacaggtatc cagccaccgc aaccagcgcg ccgaccgaca gatcgaattc cccggcgatc
     2161 atcaacaggg tgaccccgat cgctatcgtg ccggtaaggg tggcggcgct gacaatgccg
     2221 gagatcgaac gcagagtcag gaagtttggc acgaaggccg aacatagaac aaagacgacg
     2281 atgagcgtga gcagtggccc gaggatgggc gagtccgcca gcgcccgtcg cagcgggtgg
     2341 gaccagggag ccgctcgtct cactgaggct gcaatttcca tcttcgcctt ggcggagggt
     2401 ggagcttagt gggagggagg agcagcgctc ctccctccca ccagccgaac gatcgaggcg
     2461 gggagatcga gcgtcaaacg cgcagcccgc tcgcctccca cgccttgtgg actagcggta
     2521 ttgaccagcc agggccttga caatgtcgat gttgctctga tcgacaaagc ctggcccggt
     2581 tggtgtcacc ggaagcgccg gcgtgacccc atggcgcagc agcagattga gcagcatcac
     2641 tgcgccatac ccttgcagga acggctgctg atccaccgcg aagagggtcg tgccatcttc
     2701 gatggccgcg acgatctctg cactcaggtc gaacgtgccg tgcgcgatgt ctcctggtcc
     2761 aaacccagag gcctccagga aggcgtagaa cggcgtggct ccattcggcc cgagggtcaa
     2821 ggcaacgttc acctgcgggt tagcggtgaa gaagtcactg atgatcgtct gggattgtgc
     2881 cgggtcattg gtgatcgcca acacctcggc gctcagaccc ttctcggtca tcgagtcgac
     2941 gaagccctgg cagcgagcgt ccaggccggt atgacccacc tgatggttga tgcatacccc
     3001 gacctcagcc cctggctgtc ctgcaagccg ggtgccactc tggaacccgc cctggtactc
     3061 gtccgcacca aggaaggtca agtaggcgat gttgtctttg atcggcccgg caccggagtc
     3121 gtaggcgact accgggatgc cggcggcgag agcacgctcg attggctcct tgaagaggat
     3181 cggatcggtc acagtcaggg cgatgccatc ggggctggcc gccaccgcct ggtcgatcag
     3241 ggcggccgtc cgctccaggt cgaacgaatc gggggccaag acggtcgttg tgatccccat
     3301 ctgctcggcc gccagattga tgccattctc cacgacgcac cagtaggcgt cccaggagca
     3361 ggtggcgtgc tgaaccatca agagcgtgac gtcgcggtca ggatccggcg tgctgccaac
     3421 gttgctggca tccacgaatc ccgggcccgt ggcactcacc ggcagcgcgg gtgtgatgcc
     3481 gaagcgattg gtcatgaaca gccacaccac cgcgctgtaa ccctgcaagt acggctgctg
     3541 gtctatcccg aacagggtca cgccgctttc gatgttggcg gtgatctcag ggctgagatc
     3601 aaaggtgccg tggaccagat cgcccgcctc aaggccggcg gactccatcc aggcgtagaa
     3661 cgggttggca ccgttcggac cgagggtcag gaaggcatcg gtgttcgggt tggcggtggc
     3721 gtagtcaccg atgaccgttt gggattcggc cgggtcatcg ctgatcgcga gcacttcggc
     3781 ctcgagccct ttctcagcga aggcgtcgac gaagccgtta cagcgcttgt cgagcccagc
     3841 atgtccgacc tgctggttga tgcacactcc ctgggtaccg ccggccgtca ccatgcgcag
     3901 cccgcccagg tagccgccct ggtattcgtc ctggccgatg taggtcatgt aggagatccc
     3961 gtcggcgatc ggcccgtttc ccgcgttgta ggcgacgacc gggatcccgg ctgccagcgc
     4021 ccgatcaatg ggttctttaa agagcaccgg gtcggtgacg gtcaggccga tgccgtccgg
     4081 ttggccggcg acggcctggt cgatcaggga ggcggtctgc tcaaggtcga actcatccgg
     4141 gcccaaaacg gtgacctgca ctcccaggtc cgccgctgcg tcgctgatgc cctgctcgac
     4201 cgtgcaccag aaagcatccc aagcgcagag cgcgtgctgc accatgatga actcgagctc
     4261 gcgagtgagg tcaggcggcg gcgcaacctc ctgtgggacc gccgtagctt ccaccccttc
     4321 tggcggcgcc acttcggttg tggccggcgc tggcgtacag gccgccagga tcagggccag
     4381 cgtgactagc cctgcgaaca tgagacgcat ttctttcatg gatggtctcc tccttcaact
     4441 gttgattaga tgggttgtgg tctggtcgcg atcaatgtcg tgttctctag ctatcctttt
     4501 cacctcctcc ggctcgcgat gacccactcc gccagggttc acccaaggcg gtgtggcggt
     4561 agctacttta ccagtgattc ttgccttcag tgtagtccct ggccgatcac tccttagccg
     4621 accctcccag cgaggtgttc attaggacgg ccaggatcag gaagaaaccc gcgacggctt
     4681 ggaatacttg gactggaatc ctcatcaaca caagcccctg ctccagcatc tgcaacagca
     4741 ggaccccgat acaagggccc acgatcgtac cgaacccacc gctgagccgc acgcccccga
     4801 ttacgcaagc cgccacggca atcagctcca ctccgtcgcc tcgcagggga tccacgctgg
     4861 ttcgatgccc aaattgaata accccggccg cgccggccag cagcccggtg agcatgaaat
     4921 tgagcagctt gactcggttg attggcacac cctgggcatt ggccgcccgg gggttcccgc
     4981 cggtcgcaaa cacccagttt ccgtagcgcg tgcgcagcag aacaaatccg acgacggccg
     5041 cgagcagcac gaaccacagc actgagatcc gaaagttggc cggaggttga gatagccggt
     5101 tgaccgctac gattggaccg ttgagcacgt cgaagatggc cagccggggg ccgccatagg
     5161 aagcgaacac tccgccgccg atggcacgcg caatcccgcg gaacgcgagc atcgtgccaa
     5221 gcgtcgtaat gaatgaaggg atgccggact tggtgacgat ccatccgttg atcaggccga
     5281 gcaacccgct agcgatcaac gccagcagca ttgccaggat cggggcgacc ccgtcgatca
     5341 gcgctagggc gaatacatag cttgccacgg caaagatcga gcctacggat aaatcgaatt
     5401 cgccagagat catgagcagc gccacaggaa tcacgacgat gccgttgatg ctggcgaagg
     5461 tcaggatgtt cgagagggcg aagggggaga gaaagtgttc tgccgatacc gcgaaaaatc
     5521 cgaaaaccgc cgccaccgtg atgaagacca ccagttcgga ccgctcggcc agcaccgccc
     5581 gccagtcgcg caatgtgatc cccagcgaat cagccacctg catccccatc agcttccatc
     5641 tcgagcgtga cgagcggtag tcgcggatct ttctcgctcc aggtcccctt gacccagctg
     5701 taagcagggt cgtcggtgta cgccagcgac tgggcgctgc cggccaggaa gttcaggtag
     5761 taggtggtgt ac
//
LOCUS       DATDPQ010000352         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_991297, whole genome shotgun sequence.
ACCESSION   DATDPQ010000352 DATDPQ010000000
VERSION     DATDPQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991297"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..735)
                     /locus_tag="VI520_07905"
     CDS             complement(61..735)
                     /locus_tag="VI520_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37843.1"
                     /translation="MSEGRPIRAILADDHAVVRSGIRQFLETAGDIQVVAEADDGPAA
                     VEAIRANSPDVVVLDIQMPGMSGIEVTRWVRANSRETGVLILSAFDDDPYILAVLQAG
                     ANGYVLKTADAADIVAAVRAVQEGKSALDPEIARKVMAQVSGRSLTAPVERPTDRELE
                     VLGLAAKGYTNKAIGLQLGISDRTVQGHLAKTFDKLQASSRTEAVMRAVSIGWLPADL
                     GQFPEE"
     gene            complement(732..1496)
                     /locus_tag="VI520_07910"
     CDS             complement(732..1496)
                     /locus_tag="VI520_07910"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HLF37844.1"
                     /translation="MGSNSEVLTPRGRSVAIALNRAVYRLNRDWVFWFLALTGLWVVL
                     PWFAPVFMHLGWEGPAQAIYWLYSFQCHQLPQRSFFLYGPQSMLSLGRIQSLWQNTFD
                     PRVLRQFIGTAEVGFKVAWSDRMVSAYSSIPLATAIWWPLRKRLRPLPLWGFALLSLP
                     MFLDGLSHIISDLAGIDQGFRHANSWLAALTGNRLPASFYVGNALGSFNSTMRLVTGA
                     LFGAGAAWFAVPHLDLSFRESAAAIKAKFERAEVEL"
     gene            complement(1609..>2062)
                     /locus_tag="VI520_07915"
     CDS             complement(1609..>2062)
                     /locus_tag="VI520_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF37845.1"
                     /translation="KGSMPAWQGRLSQDERWAVIDYLRAFSYDPALAGEAVVAAPPTA
                     ASAAVCDATYLTQSNPFAWEDATAAASGEAIYKEACEVCHAADGSGALPSAPDFSTAA
                     FQSELSSSQGGELLCVVAEGRGAMPGWKETLTLEQIWQVLTFIASLGK"
BASE COUNT          405 a          648 c          670 g          339 t
ORIGIN      
        1 agctgaaagg gagaatgatc aggccgagca gctgcaagtg cagcccgccg acgcgcagct
       61 tcattcttcg gggaattgtc cgagatcggc agggagccag ccgatggata cggcgcgcat
      121 gaccgcctcg gtccggctgg aggcctgcag cttatcgaaa gtcttggcca gatggccctg
      181 gacagtgcga tcgctaatgc ccaactgcag cccgatcgct ttgttggtgt agcccttggc
      241 tgccaggccg agcacctcga gttcccggtc ggtcgggcgc tctaccggag cagtgaggct
      301 gcggcccgag acctgggcca tcacctttct ggcaatctcc ggatcgagcg ccgatttgcc
      361 ttcctgcacg gcccgcacgg ccgcaacgat gtcggccgcg tcggcggtct tcaatacgta
      421 gccgttggcg cccgcttgca ggacagccag gatataaggg tcgtcgtcaa aggcgctcaa
      481 tatgagcacc ccggtttctc gagagtttgc ccgcacccag cgggtgacct cgatcccact
      541 cattccaggc atttgaatat ccagcactac aacatccggg gaattggccc gaatggcctc
      601 caccgcggcg gggccatcat ccgcctccgc caccacctgg atgtcccctg cggtttccag
      661 gaactggcgg atgccggacc ggacgacggc gtgatcatcg gccaggatcg cccggatcgg
      721 gcgaccctcg ctcacaactc gacctctgct cgttcgaact tggccttgat cgccgcggcg
      781 gattcccgga aactcagatc gagatggggg acggcaaacc aagccgcgcc cgctccaaag
      841 agggccccgg tgaccaaccg cattgtggag ttgaacgagc ccagggcgtt tcccacgtaa
      901 aaggaggcgg gcaagcggtt gccagtcagc gcggccagcc aggagttcgc gtggcgaaag
      961 ccctggtcga tgccggcaag gtcgctgatg atatggctca ggccatccaa aaacatcggc
     1021 agggagagca gcgcgaaacc ccagaggggt agtgggcgca accgcttgcg caggggccac
     1081 cagatagcag ttgccaatgg gatgctggag taggctgaga ccatccggtc cgaccaggcc
     1141 accttgaaac cgacctcggc ggtccctatg aactgtcgca gcactcgcgg atcgaaagtg
     1201 ttctgccaga gcgactggat tcggcccagg gagagcatcg attgggggcc gtacagaaag
     1261 aaggaacgct gggggagctg atggcactgg aaagaataca gccagtaaat ggcctgggca
     1321 ggtccttccc agcccaggtg catgaacacc ggcgcaaacc acggcaggac gacccacaac
     1381 ccggtcagcg ccaggaacca gaagacccaa tctcggttga gtcgatagac tgcccggttg
     1441 agggcgatcg ctacagatcg cccgcgaggg gtgaggactt cggagttgga tcccatacgt
     1501 tgctgactac cgcccatgat tctaggccag gtcgcagagg gcggcgaggg tcaactccac
     1561 gggcggcggc caggtcaaat ggcgcggact ggggcgaccg ctttcagctc atttgcctag
     1621 gctcgcaatg aaggtcagca cttgccagat ctgctccaga gtgagggtct ctttccagcc
     1681 cggcatcgcc cctcgcccct cggcaaccac acagagcaac tcaccgcctt gggaagaact
     1741 gagttcgctc tggaaagccg cggtgctgaa gtcgggcgcg ctgggcagcg cgccggagcc
     1801 gtcggccgca tggcatacct cgcaggcctc cttgtagatc gcctcgccgc ttgcggcggc
     1861 ggtcgcatcc tcccaagcga acggattcga ttgcgtgagg taggtggcgt cgcacacggc
     1921 cgccgacgcg gcggtcggtg gggctgcgac gacggcctcg cctgccagag cgggatcgta
     1981 ggaaaaggcg cgcaggtagt cgatgaccgc ccagcgttcg tcctgcgaga ggcgtccctg
     2041 ccaggccggc atcgagcctt tc
//
LOCUS       DATDPQ010000353         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2554257, whole genome shotgun sequence.
ACCESSION   DATDPQ010000353 DATDPQ010000000
VERSION     DATDPQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554257"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VI520_07920"
     CDS             complement(<1..530)
                     /locus_tag="VI520_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37846.1"
                     /translation="MADPTAYEIRQGAYYDSVVLMQLQRALTGLPGVRDAGVVMATPA
                     NLELLAQNDLLPDQIDPAPEDLLIVVRADSQAPAEQALAQVDELLARRRTVQAGHYRP
                     RSLKAAVEMLPQAEWILISVPGRYAGAVAREALDLGRNVFLYSDNVPVEQEVELKQSA
                     AAKGLLLMGPDCGTAIV"
     gene            complement(597..1895)
                     /locus_tag="VI520_07925"
     CDS             complement(597..1895)
                     /locus_tag="VI520_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLF37847.1"
                     /translation="MGLWLQVMPPLTAILDSLRSGHRGFLEELQVRFETVEPELHAFL
                     PEERRFERLHTEFGELERRFPAADDRPPLFGLPIGVKDIFRVDGFETRAGSRLPPASL
                     AGQQAVCASQLKQAGGLVLGKTVTTEFAYFSPGPTRNPHHPGHTPGGSSSGSAAAVSA
                     GLAPTALGTQTIGSINRPAAFCGVVGFKPSFGRISTQGVIPLSASFDTVGFFTQEVEA
                     ARLMASILIADWRPAAVGQKPVLGVPTGPYLDKASTEGRTHLERVIEQLRRAEFHVVE
                     VPAMPDYPEIVERHNRVLAAEAAEVHREWFSKFEPLYHAKTAELIRRGQAVRPSELAA
                     GRAGGEELRAELSRLMGQGGLDLWISPSAMGPAPEGLESTGDPAMNLPWTHAGLPTIS
                     LPTGRSEDGLPLGTQLAGGWQRDGQLLGFAAMIETIVGES"
     gene            complement(1901..>2301)
                     /locus_tag="VI520_07930"
     CDS             complement(1901..>2301)
                     /locus_tag="VI520_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF37848.1"
                     /translation="LAGINAGGAQAMPRVVHFELGVADPERAAQFYRAVFGWKVEKWG
                     DFDYWLVTTGEDSEPGINGAILVNKDAKPRTVNTVQVESIEDAAEKVLDAGGAVVTEK
                     VTIPNVGHNLFCKDTEGNLFGLHQVDPQAK"
BASE COUNT          395 a          789 c          701 g          416 t
ORIGIN      
        1 acgatagccg ttccacaatc tggtcccatg agcagcaggc ccttcgcggc cgcggactgt
       61 ttgagctcaa cttcctgttc gaccgggaca ttgtcgctgt aaagaaagac atttcgtcca
      121 aggtccaggg cctctcgggc cacggctccc gcgtaacggc cgggaaccga aatcagaatc
      181 cactcggcct gcggaagcat ctcgaccgca gctttcaagc tgcgggggcg gtagtggcca
      241 gcctgaacgg ttcgccttcg ggcgagcagt tcgtcgacct gtgccagggc ctgctcggca
      301 ggagcctggc tgtcggcccg caccacgatg agcaagtctt ccggagccgg gtcgatctgg
      361 tccggcagca ggtcgttctg ggcgagcaac tccaggttgg caggggtggc catcaccact
      421 cccgcgtcgc gcactccagg caagccggtc agcgcccgct gcagctgcat caggacgacc
      481 gagtcgtaat aggcgccctg ccgaatctcg taagcggtcg ggtcagccat tccggggtga
      541 cccgcgcgga gctattgtga aattcctcac gaagtgggtc tcgggggctg caacgctcag
      601 ctctccccca cgatggtctc gatcatcgcg gcgaatccca gcagctgccc gtcccgctgc
      661 cagcccccgg ccagctgggt gccgagcggc aagccgtcct cgctgcgccc ggtcggcaag
      721 ctgatcgtcg gcagcccggc gtgggtccac ggcaaattca tcgcaggatc gccggtgctc
      781 tccagaccct ccggcgccgg ccccatcgcg gacggggaga tccacaggtc gagcccgccc
      841 tgacccatca gccggctgag ctcggcccgc agctcttctc caccggcgcg cccggccgcc
      901 aattcggacg gcctcacagc ctgaccccgc cgaatcagct cggcggtctt ggcatggtac
      961 agcggctcaa acttgctgaa ccactcgcgg tgaacttcgg ccgcctcggc cgccaacacc
     1021 cgattgtgcc gctccacgat ctcggggtag tccggcatgg caggcacttc gacgacatgg
     1081 aactcggctc tgcgcaattg ttcgatgact cgctccagat gggtccgccc ctcggtcgag
     1141 gccttgtcaa ggtacggccc ggtggggacc ccgagcaccg gtttctggcc tactgctgcc
     1201 ggtcgccaat ctgcgatcag gatcgaggcc atgagccgcg ccgcctcgac ctcctgggtg
     1261 aagaaaccaa cggtatcgaa cgaagccgag agcgggatca cgccctgagt ggagattcgg
     1321 ccgaagctgg gcttgaagcc caccacccca caaaaggccg ccggacggtt gattgagccg
     1381 atggtttggg tccccaatgc ggttggtgcc aatccggccg agacggccgc cgccgatccg
     1441 ctgcttgacc ctccgggggt atggcccgga tggtgtgggt tgcgggtggg gccgggggaa
     1501 aagtaggcaa actcggtcgt caccgtcttg cccagcacta atccgcccgc ttgtttcagt
     1561 tgggaggcgc agacggcttg ctggccggcc aagctcgcgg ggggcagccg gctaccagcc
     1621 cgggtctcga agccgtcgac cctaaagatg tccttcaccc cgatcggaag cccaaagagt
     1681 ggaggccggt catccgcggc tggaaaacgc ctctcgagct cgccgaattc tgtgtgcagc
     1741 cgttcgaagc gccgctcttc tggcaggaac gcgtgcagtt caggctcgac cgtctcgaac
     1801 cgcacttgca gttcttccag aaaccctcga tggcccgatc ggagcgaatc caggatcgcg
     1861 gtcagcggcg gcatcacctg cagccagagt cccactgcac tcacttggcc tgggggtcga
     1921 cctggtgtaa gccgaacaga ttcccctccg tatctttaca gaacaggttg tgcccgacgt
     1981 tggggatcgt cactttctcg gtcaccaccg cgcctcctgc gtcgagcacc ttctcggccg
     2041 catcttcgat cgactcgact tgcaccgtat tcaccgtgcg gggtttggca tccttgttga
     2101 ccaggatcgc tccgttgatg cccggttcgc tgtcctcacc ggtcgtcacc agccaatagt
     2161 cgaaatcccc ccacttctcg accttccaac cgaaaacggc tcgatagaac tgggcggccc
     2221 gctcgggatc ggccaccccc aactcaaaat gaactactct cggcatcgcc tgcgctcctc
     2281 ccgcattgat tccggcgagt g
//
LOCUS       DATDPQ010000354         3704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_720547, whole genome shotgun sequence.
ACCESSION   DATDPQ010000354 DATDPQ010000000
VERSION     DATDPQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720547"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1307)
                     /locus_tag="VI520_07935"
     CDS             complement(177..1307)
                     /locus_tag="VI520_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37849.1"
                     /translation="MTGSDSAESHLPGGIRLPFEIQVEPRLHVPRWLSPLISVIAILV
                     ALLLGALVLALVGGDPVRAYAHIAGAAFGSLGVFSDTLVKATPLIMTGLACSVAFQMK
                     LWNIGAEGQFYMGALGASIVILAGVVPADAPRLVAIPIMLVAGVVFGAAWGLVPGWLK
                     ARLRVNEIITSLMLNYIAIALVNYFIFGVWSEGGFQMTRTFQQAAWLPRLSDLAEKIP
                     ILRGLTTHFGLVFAVVAAVIVWYILFRSLWGYEIRLIGDNPRAAKYAGINITRNTVFV
                     MMLSGGIAGLGGMSEIAGVVHRLQGSISPGYGFTGIIIAWLAKLNPLVVLPVSVLFGG
                     LILAGREIQPAGVPRLIQGIILFMLIASEVFLRYRIRLVRRP"
     gene            complement(1304..2839)
                     /locus_tag="VI520_07940"
     CDS             complement(1304..2839)
                     /locus_tag="VI520_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37850.1"
                     /translation="MAEDANLVEMRGIVKRFPGVLANDEVDFELRPGEIHALLGENGA
                     GKSTLMSVLAGLYKPEAGTIRVRGVPVSFGSPRDAIALGLGMIHQHFTLIDSHTVTEN
                     ILLGLDDPRFLMRLARYDERIAQTAERFGLHVAPSAKIWQLSVGEQQRVEILKMLYRG
                     VQVLIMDEPTSVLAPQEIDQLFKTLRSMVDQGKSIVFISHKLHEAMSIADRISVLRHG
                     KVTAAGIKPEATSKAELANLMVGRKVLFEVEKKPRQPGEVVLELRGVCAENDKGLPAL
                     RDVGLKLRSGEILGLAGVAGNGQSELGEVITGLRRCTSGSILVNGKEVANRPARVPIE
                     LGVAHIPEDRTHVGTAPNLSITDNVIMKGYRDPPLAKGRTLDRQAARERAERLKAEYT
                     ILAPSVDTPARLLSGGNLQRVILARELSAEPTLLIAMQPTRGLDVGAIEGVQRLLLEQ
                     RDSGAAILLVSEELEEIFSLSDRIAVIYEGEIVGDSVAADAETVGLLMTGGAGSPKTE
                     AAA"
     gene            complement(2994..>3704)
                     /locus_tag="VI520_07945"
     CDS             complement(2994..>3704)
                     /locus_tag="VI520_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLF37851.1"
                     /translation="KTDGNPRIGYMATFPIPEELRLGNAIMLGVKRTCPECTMDVRWI
                     NTWHDPVIEKEGAASLFDAGAQVVFTGADTPAAGDVAMEKEKWGVNYDHVGACKVDRC
                     LTAPYWTWGPIYARITEETIAGTHVPGFQYFDSSEGGMGLYGFMEGQTLQPGVADLPA
                     EDLQLVRDTLAAALAGDFNRFDVFKGPISDNQGNVILADGVSLEQLDLDGFAVFGSPC
                     QTCMYWWAEGITAELPDL"
BASE COUNT          738 a         1201 c         1077 g          688 t
ORIGIN      
        1 ggttgccacg gtgacgctga atgcgctcat cgcgccaatc agcatcatcc cctccagacc
       61 cagattcaat actccggccc gctcggcaaa gatctccccg atggttgcga agagtaggac
      121 cgttccggtc gccacccccg cgtggaggat aatgagggga tccacggttc accgcttcac
      181 ggccgacgca cgagccggat ccgataacgt agaaacactt cgctcgcaat gagcatgaac
      241 agaatgatcc cttggatgag ccgaggaact ccggcgggtt ggatttcgcg cccggccagg
      301 atgaggcctc cgaagagcac cgatactgga agcacaacaa gcggattgag tttggcgagc
      361 caggcgatga tgatcccggt gaatccgtaa cccggtgaaa tcgacccctg cagccgatgg
      421 acgacaccgg caatttcgga catcccaccc agcccggcaa taccccctga cagcatcatc
      481 acgaaaactg tgttgcgggt gatattgatg cccgcgtact tggccgctcg cgggttatcg
      541 ccgatcaggc ggatctcata accccacagg ctgcggaaga ggatgtacca cacgatgact
      601 gcggccacga ccgcgaacac caggccgaag tgggtggtca gccctctgag gatcgggatc
      661 ttttcggcca ggtccgagag gcgcggcagc caagcggctt gctggaaggt gcgggtcatc
      721 tgaaacccac cctcgctcca aaccccgaag atgaagtaat tgaccagcgc aatcgcgatg
      781 tagttgagca tcaggctcgt aatgatttcg ttcacccgga ggcgggcctt gagccagcct
      841 ggcaccagcc cccatgccgc tccgaagacg accccggcga ccaacatgat cgggatcgcg
      901 acgagccgcg gagcgtctgc tggaaccact ccggccaaaa tgacaatgct cgccccgagg
      961 gcgcccatgt agaattggcc ctcggcccca atattccaga gcttcatttg aaaggcgacc
     1021 gaacatgcca gcccggtcat gatcagcggg gtcgctttca ccaaggtgtc ggagaatacc
     1081 cctaagctgc caaacgccgc gccggcgatg tgagcataag ctctgaccgg gtctcctccg
     1141 accaatgcaa gcaccaacgc acccagcagc agagcgacca ggattgcgat gactgatatc
     1201 agcgggctca gccaacgcgg cacgtgcagg cgcggctcaa cttgaatttc gaaaggaagc
     1261 cggatgccgc ccggcagatg ggattcggcc gaatccgaac ccgtcacgcg gccgcctcgg
     1321 tcttcggcga ccctgcccca ccggtcatca atagcccaac cgtctcggcg tccgccgcca
     1381 cgctatcccc cacgatctca ccctcgtaga tcactgcgat ccgatcgctg agcgagaaga
     1441 tctcttccag ctcctctgag accagcagga tggcagcccc cgagtcgcgt tgttcgagca
     1501 gcagacgttg aactccctcg atcgcgccga cgtcaagccc gcgagtgggc tgcatggcta
     1561 tcagcagagt tggctcggcg gaaagctcgc gtgccaggat gacccgctgc agattgccgc
     1621 cggatagcag cctggccggc gtatcgacgc tgggggccag aatggtgtac tcggctttaa
     1681 gccgctctgc ccgttcgcgg gccgcctgcc gatcgagagt tcggcccttc gccagtgggg
     1741 gatcgcgata tcccttcatg atgacattgt ctgtgatgct caggttggga gcggtcccga
     1801 cgtgggttcg atcctcggga atgtgggcga ccccaagctc gatcggtacc cgcgccgggc
     1861 gattggccac ctccttcccg ttcaccagta tgctgccgct cgtgcagcgc cggagcccgg
     1921 taatcacctc tccgagttcg ctttgaccat tgcctgcgac accggccagt cccaagatct
     1981 cgccggatcg cagcttgagc ccgacgtcac ggagcgccgg caaccctttg tcgttttcgg
     2041 cacagacccc ccgcagctcc aagaccacct cccccggctg cctcggcttc ttttccactt
     2101 caaatagcac tttgcggccg accatcaggt tggccagttc cgccttggag gtcgcttccg
     2161 gtttgatccc ggccgcggtg accttgccgt gacgaagcac gctgatccga tccgcgatgg
     2221 acatggcctc atgcagcttg tggctgataa acacgatcga cttgccctga tcgaccattg
     2281 aacgcagggt cttaaatagt tggtcaatct cctgcggggc aagcacggag gtaggctcat
     2341 ccatgatcag gacttgcacc ccgcgatata gcatcttcag gatttcaacc cgctgctgtt
     2401 cgcccaccga tagctgccag atcttggccg agggagcgac atgcagccca aaccgttcgg
     2461 ccgtttgcgc gatccgttcg tcatagcggg ccagccgcat taggaatcgt gggtcgtcta
     2521 gcccgagcag gatgttttcg gtgaccgtat gcgaatcgat gagggtgaag tgctggtgga
     2581 tcatccctag gccgagagcg atggcatccc gtggtgaacc gaagctcacc ggcacacctc
     2641 gaacccggat ggttcctgcc tctggcttgt acaaaccggc cagcacgctc atcagggtgc
     2701 tcttccccgc gccgttctct cctaacagag cgtgtatttc tccgggccgg agctcgaagt
     2761 ccacctcgtc gttggccagc acgcccggga aacgcttgac gattccccgc atctccacca
     2821 ggtttgcgtc ttctgccatc ggattcatcc tcagcggggc cggaacgacg ggcggcgggg
     2881 caaggcagcc ctggcggcgc gggcagtccg cgccgccagg gtcggggtca atcctctcct
     2941 aagctgtggg ccctaagaaa cgagtacgca gcggactgcc tgcgagctca gttctataga
     3001 tcgggcagct cggccgtaat gccttccgcc caccaataca tacaggtctg acatggtgag
     3061 ccgaatacgg cgaagccgtc gaggtcaagc tgctccagcg acacgccgtc ggccaagatc
     3121 acgttgccct gattgtcaga gattgggccc ttgaacacat cgaagcgatt gaagtcgccg
     3181 gctagggcgg cggccagcgt atcccggacg agctgcaagt cttcggcggg aaggtcagca
     3241 acgcccggtt gaagggtctg gccctccata aagccgtaga gccccatgcc accctcggaa
     3301 gagtcgaagt attggaagcc cggcacatgg gtcccggcga tcgtttcttc cgtgattcgg
     3361 gcatagatcg ggccccaagt ccagtagggc gcggtcaggc agcgatctac cttacaagcg
     3421 ccgacatgat catagttgac gccccacttc tccttctcca ttgccacgtc accggccgcc
     3481 ggagtatccg caccggtaaa cacgacctga gccccagcat cgaagagcga ggccgcccct
     3541 tccttctcga tcaccggatc gtgccaggtg ttgatccagc gaacgtccat cgtgcactct
     3601 gggcaggtcc gtttaacccc gagcatgatc gcattgccca ggcgcagttc ctcagggatg
     3661 gggaaagtgg ccatgtatcc gatcctcgga ttgccatcgg tctt
//
LOCUS       DATDPQ010000355         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2047649, whole genome shotgun sequence.
ACCESSION   DATDPQ010000355 DATDPQ010000000
VERSION     DATDPQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047649"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VI520_07950"
     CDS             complement(<1..194)
                     /locus_tag="VI520_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLF37852.1"
                     /translation="MAAKPIVDGIEQEHGSKLRVIRLNIQDRAGEQLAARFGLQFTPT
                     FVLLNAQGEVIGRWAGALDPG"
     gene            complement(204..461)
                     /locus_tag="VI520_07955"
     CDS             complement(204..461)
                     /locus_tag="VI520_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37853.1"
                     /translation="MSAGQFINQHSFTIVGGAALILLAVALLRDGIEPSDLVALGALL
                     LGLTLSYSLFNPGKSTETDPEAVLREIGAGTPVLLELQSLY"
     gene            548..>2043
                     /locus_tag="VI520_07960"
     CDS             548..>2043
                     /locus_tag="VI520_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLF37854.1"
                     /translation="MKDYPTQALRNIALVSHGGAGKTSLGEAMLYHTGALTRMGTVQE
                     GTSVSDFEQEEVRRQQSLSTSVLPIEFREHKINLLDTPGFLDFVGEAISALWVVEGAV
                     LLVDSVAGVEVGTELMWKYCDEFKLPRFVAISKMDRDNADFGKVLSTLPALSGSVTFV
                     PVQLPWGEKQGFRGVIDLVRMTARPAEGSKEEAIPPEFSEAAEAGRTALVEAAAEGDD
                     ALLEKYLEGEPLTADEVSRGFRRAVLSGRFVPVFATAGTAGAGVIPLLEAIAALIPSP
                     SEAAPRTAAGPGGEVTLDGGDTGPLAAYALKTTADPFVGRLTYLRLFSGTMSSDSRVW
                     NPARREEERLGTLHLMRGKEQLPTVNLHTGDIGVVAKLSATQTGDTLCDKTMPISIPS
                     PTYPNGLYSVAVLPKTQADAAKISSVLSRLVEEDPTLRWHQEQSTNETILQGMGDQHI
                     DAAIRKAESKFQVGLTTGVPRVPYRETITKVGNSMYRHKKQTGGSGQFA"
BASE COUNT          396 a          632 c          649 g          366 t
ORIGIN      
        1 cccggatcga gagcacctgc ccaccggccg atcacctcgc cctgtgcatt caataacacg
       61 aacgtggggg tgaactgtaa gccaaaccgg gccgccagct gctctccggc ccgatcctgg
      121 atgttcagcc ggatgactcg caacttggac ccatgttcct gttcaatccc atccacgatg
      181 ggtttggctg ccatgcagcc tgctcagtag aggctctgta attcgagcag gaccggggtg
      241 ccggcaccga tctcccgcag cactgcctcg ggatcggtct cggtgctctt acccgggttg
      301 aagaggctgt aggagagggt gagccctaga agcagtgccc cgagcgccac caaatcggat
      361 ggctcaatcc catcccgcag cagagcgacg gccaacagga tcagggccgc cccgcctacg
      421 atggtgaacg agtgctggtt gataaactgg ccggcagaca acgggcggat tgtatcatcg
      481 cgggtttcgt tgacatcccc atacccctct tttacaatgc ttcacccccc ggtctcgagt
      541 tcagcggatg aaggattacc caactcaagc actgcgcaac atcgctctgg tatcgcatgg
      601 cggggctggc aagacttcgc taggggaggc gatgctctac cacaccggag ccctcacccg
      661 gatgggtact gtacaggagg gcacctcggt ctccgatttc gaacaggagg aggtccggcg
      721 ccagcagtcg ctttcgacca gcgtcttgcc gatcgaattc cgagagcaca agatcaatct
      781 gctggacacg ccgggcttcc tggattttgt cggcgaggcg atctcggccc tctgggtggt
      841 ggagggtgcc gtgcttctcg tcgactcggt ggccggggtt gaggttggga ccgagctgat
      901 gtggaagtac tgcgacgagt tcaaacttcc gcgctttgtg gccatcagca agatggaccg
      961 cgacaacgcg gatttcggca aggttttatc gacgcttccc gctctttccg gcagcgtgac
     1021 ctttgttccg gttcaactcc cgtggggcga aaagcaaggc ttccgggggg tgattgatct
     1081 agttcggatg acggcccggc ccgccgaagg gagcaaggaa gaagctattc cgcccgagtt
     1141 ctcggaggcg gctgaggcag ggcgtaccgc gctggttgaa gccgcggccg agggggacga
     1201 cgcgctgctg gagaagtatc tcgaaggcga gccgctcacc gcggatgaag tctcgcgagg
     1261 cttccggcgg gccgtgctct caggacgctt cgtcccggtc ttcgcaaccg ccggaacagc
     1321 cggggccggg gtcatcccgc tgctcgaggc gatcgctgcc ctcatcccgt caccctccga
     1381 agcggctcct cggaccgccg ccggacctgg cggagaggtc accctggatg ggggagatac
     1441 gggaccgctc gcggcctatg ctctgaagac gaccgccgat ccgtttgtcg gccgcctgac
     1501 ctacctgcgg ctattctcgg ggacgatgag ctccgacagt cgggtgtgga atccggcgcg
     1561 gcgcgaggaa gagcgcctgg gaaccttaca cctcatgcgg ggcaaagaac agctgccaac
     1621 ggtgaacctg cacaccggcg acattggggt ggtcgcgaaa ttgagtgcga cccagactgg
     1681 cgataccctg tgcgacaaga ccatgccgat ctcgatcccc tccccgacgt accccaacgg
     1741 gctgtactca gtggcggtcc tgccgaagac ccaggcggac gcggccaaga ttagctcggt
     1801 cctcagtcga ctggtcgagg aggatcccac cctccgctgg caccaggaac agagcaccaa
     1861 cgagacgatt ctgcaaggga tgggtgatca acacatcgat gccgcgattc gcaaagccga
     1921 atcgaaattt caggtcgggc tgacgaccgg ggtccccagg gtcccgtatc gggagaccat
     1981 cacgaaagta gggaactcca tgtaccgcca caagaagcag accggcgggt ccggccagtt
     2041 tgc
//
LOCUS       DATDPQ010000356         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2556041, whole genome shotgun sequence.
ACCESSION   DATDPQ010000356 DATDPQ010000000
VERSION     DATDPQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556041"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VI520_07965"
     CDS             <1..1497
                     /locus_tag="VI520_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLF37855.1"
                     /translation="QAYAGGQVSMDRMVVDFQLQPGVAWSDGQPVTAQDSVFSFELDR
                     QVDTPTPKTQVDRTATYEALDARSVRWTGIAGYTDSEYFSNFWSPLPQHVLGDRSALD
                     LLRAEDTTETPIGWGPFVIEEWRAGEDIQLSKNPNYFRAAEGLPAYDYLLFRFLKDGS
                     AGSIEQLLTSECDVLDESAVADGFNVELLDEAALSFLVDLETGGRVQLSAAFGPELER
                     LEFGLAPAPGAGMSPIFSDPRTRAGIAACLDREGFVSEVLHGLSAVPATYLSPEHPLY
                     AGEIASVEFNPQEGIRLFEEAGWIDADGDPATARVAQGMAGVQPGTELSFTFLTTPDG
                     FHRSAAEQFRADLVACGVDLVVDLLPAAELFTPWPEGPAFGRTFQTLGWAWPGWVSPL
                     CEMFAGWEIPTGENPFGANASGFTDPAYDAACAVLLHGQPGSPEYLTATQETQAIFAE
                     QLPAIPLYMRPRVIAHGPEICGLETDPTAFSALWNIEEIRPCGPDPTP"
     gene            1567..2148
                     /locus_tag="VI520_07970"
     CDS             1567..2148
                     /locus_tag="VI520_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLF37856.1"
                     /translation="MTDQGDLQLILQLRQGDLEALGGLYDRHRHLVYRTALAITGDTE
                     ASADLLHDTFLRLYRFGHRIDPTRPIEPWLYRMAANMAYTWVKRRGRWYQALREMADR
                     LSREYPTSPAQQVEKNEAWMEIRRALQAISAEKRVVVVLYYLNDLPISEIAEILDLPL
                     GTVKSRLHYGREALKKQLGLVGQVVPRVGYEAT"
     gene            2135..>2327
                     /locus_tag="VI520_07975"
     CDS             2135..>2327
                     /locus_tag="VI520_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37857.1"
                     /translation="MRRPSIHPPDERIAFSLRGGLRGIEPPTELRLKLLAAAAQESSL
                     RGGGWRSRALPGWTSASTAR"
BASE COUNT          407 a          723 c          753 g          444 t
ORIGIN      
        1 caggcgtatg cgggcgggca agtctccatg gatcggatgg tggtggattt tcagttgcag
       61 ccgggggtcg cgtggtccga cggccaaccg gtcaccgcac aggattcggt gttctccttc
      121 gagctggacc gccaggtcga cacgcccact ccgaaaactc aagtagatcg gaccgccact
      181 tacgaagcgc tcgatgctcg gagcgtccgg tggaccggga tcgccggcta taccgactcg
      241 gagtacttct ccaacttctg gagcccgctc ccccagcatg tgctcgggga ccgaagtgcg
      301 ctggacttat tgagggcgga ggacaccacg gagaccccaa tcgggtgggg cccgtttgtc
      361 atcgaagagt ggcgagccgg ggaagacatt cagctgagca agaaccccaa ctattttcgt
      421 gcggccgagg ggctgcccgc ctacgattat ctgctctttc gcttcttgaa ggatgggtcg
      481 gccggcagca tcgagcagtt gctaacgagc gaatgcgatg tgctggacga atcagcagtc
      541 gccgatgggt tcaacgtcga gctgctggac gaggcggcgc tctcgttcct ggtggacttg
      601 gaaactggcg gtcgcgttca gctgtcggcc gccttcggac cggagctgga gcgcctcgaa
      661 tttggcctgg cgccggcgcc gggggcagga atgtccccga tcttttccga tccgcgcacc
      721 cgggccggaa ttgcagcttg cctggatcgg gagggcttcg ttagcgaagt gctccacggg
      781 ctcagcgcag ttccggcaac ctacctgtcc ccggagcacc ccttgtatgc cggcgagatt
      841 gcctcggtcg aattcaatcc tcaggaagga atccggctct ttgaggaggc cggctggata
      901 gacgccgacg gcgatccggc caccgcccgg gtggcgcagg gcatggcagg ggtgcagccg
      961 ggaaccgagc tctcgtttac attcctgacc acgccggatg gattccaccg cagcgcggct
     1021 gagcagttcc gagccgacct ggtcgcctgt ggggtggacc tggtggtcga cctcctgccg
     1081 gcggccgagc tctttacgcc ctggccggag ggtccggcgt ttggtcgcac tttccagact
     1141 ctgggctggg cctggccggg ctgggtgagc ccactgtgcg agatgttcgc cgggtgggaa
     1201 atccccaccg gggagaaccc ctttggtgcc aacgcttccg gcttcaccga cccggcctac
     1261 gacgcggcct gcgccgtact gctccatggg cagccgggtt cccccgaata tctaaccgcg
     1321 acccaggaga cccaggcgat attcgctgag caactacccg ccatccccct ctatatgcgg
     1381 ccccgggtca tcgcccatgg gcctgagatc tgcgggttgg agaccgaccc cactgcgttt
     1441 agcgcccttt ggaacatcga agaaatccgt ccctgcggcc cggatccgac tccctgaacc
     1501 ggtggcctgc cttgctggta tatttgttta acaagtggta gacgataaat acgcccggag
     1561 tcggtcgtga ccgaccaggg cgacctgcaa ctgatcctcc agctcaggca gggggaccta
     1621 gaggcgcttg gtggtctgta tgaccgccac cgccacttgg tctaccgtac cgcgttggcc
     1681 atcaccggcg acacggaagc ctcggccgac ctgctgcacg acaccttcct tcggctctat
     1741 cgattcggcc accggatcga tcccacccga ccgatcgaac cctggctata ccgcatggcg
     1801 gccaatatgg cctacacctg ggtaaagcgc cgagggcgct ggtatcaagc gctccgtgag
     1861 atggctgatc ggctctcgcg tgagtatccc acctcgccag cccagcaagt cgaaaagaat
     1921 gaagcctgga tggagattcg cagagcgctg caggcgatct cagcagagaa acgggtggtg
     1981 gtcgtcttgt attacctgaa cgacctgccg attagcgaga ttgctgagat cctggatctg
     2041 ccgctgggca ccgtgaaatc tcgcctccat tacgggcggg aggccttgaa aaagcagctg
     2101 ggcctcgttg gacaggttgt gccacgggtg ggttatgagg cgacctagca ttcaccctcc
     2161 ggatgaacgg attgccttct cgctgagagg cggcctgcgg ggtattgagc caccaaccga
     2221 gctccgattg aagctgttgg ctgcggccgc gcaggaatcc agtttgcggg gaggaggctg
     2281 gaggtcacgg gccctcccgg gttggacttc ggcctcgacg gcccgcc
//
LOCUS       DATDPQ010000357         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_454129, whole genome shotgun sequence.
ACCESSION   DATDPQ010000357 DATDPQ010000000
VERSION     DATDPQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454129"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VI520_07980"
     CDS             <1..582
                     /locus_tag="VI520_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37858.1"
                     /translation="TKALKEFFRERPELILLDVMMPGMDGWEVCARVREMAETPIIML
                     TAKSSEADKLRGFRLGVDDYLAKPFSLAELAARIKAVLARASTGPTEELQLHSVGAIA
                     VDTRRMEATLNGQPLALTPTEFRLLAALASRPGEAVSQQDLTAAVWRNLQEEGGSALR
                     RYVWFLRQKIETDPEKPHRLVTVRGYGYRLEEG"
     gene            616..1920
                     /locus_tag="VI520_07985"
     CDS             616..1920
                     /locus_tag="VI520_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF37859.1"
                     /translation="MVERREARTDLPQAIEEAVAQAVGLIGDKPVTVVAEYPAHMPAV
                     RVDRQRLVSLIGGVVGHAVDAAEQGEVVVTAEIWASGSRLEGNPAENPWAVVQVKLPA
                     SEQAARRLADGLQPTASSSRTPQLLIPSLPSLPSIQAMAGGLGGNLILEYPEPDQPSF
                     RLSLPLLAAPRGEGDLRSIVEYHLSGEPKSGETLLLVVDEPARLAEMTAELVEEGYSV
                     IPTSNGGEVLGLARREQPDLILLDMLARDPGALDVAMVLKQDSRTLNIPVLFLTSTSD
                     PQGERLGAASFLVRPTGTGALVSTIQAVLGAGLSPAGRVLVAEQDDIAREFLVMMIQA
                     HGYRVTEAAGAEEALALAEHIPPELVLVNSDMAHERDYWLLRGLRQLGQGFDIFVLAE
                     AITEAEGQEAVDRGASGFSQTGQLPALLDQVRKGKPRPQKLS"
     gene            complement(1913..>2263)
                     /locus_tag="VI520_07990"
     CDS             complement(1913..>2263)
                     /locus_tag="VI520_07990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLF37860.1"
                     /translation="SGIPDSRPRENGIPTITPSGQFFIEVKMPSRHMGDGRITADLEA
                     YELPGVPWVSFFTATGIAFHGTYWHNDFGRPMSHGCINMDTREALWLYRWTLPVVQPD
                     EKHRIGRGTPIYIS"
BASE COUNT          410 a          687 c          768 g          398 t
ORIGIN      
        1 accaaggctc tcaaggagtt cttccgcgag cggccagaat tgatcttact ggacgtgatg
       61 atgcccggca tggacggttg ggaggtctgt gcccgggtgc gggagatggc cgaaacgccc
      121 atcatcatgc tcaccgccaa atcgtccgag gcagacaaac tgagaggatt ccgactggga
      181 gtggatgatt atctggctaa accgttcagc ctagccgaac tggccgcccg aatcaaggca
      241 gttctggcgc gcgccagcac gggtccgacg gaggagctgc aactccacag tgtgggggcc
      301 attgcggtgg acacccggcg gatggaggcc accctgaatg gtcaaccgct tgccttgacc
      361 ccgaccgagt tccgattgct ggcggctctg gccagccggc cgggggaggc cgtttctcag
      421 caggacctca ccgccgcggt ctggcgcaat ctgcaggaag agggcggcag tgcgttacgc
      481 cgctacgtct ggttcttgcg gcagaagatc gaaacggatc cggaaaaacc acaccgcctg
      541 gtgacggtac gtggatacgg gtaccgcttg gaggaggggt aggtcgggtg cggcggccac
      601 tccgagaggt catccatggt tgagcggcga gaggcgcgca ccgatcttcc tcaggcgatc
      661 gaggaagcgg tcgcccaggc agtcggtttg atcggcgaca agccggttac ggttgtggcc
      721 gagtatccgg cccacatgcc tgcggttcgg gtggaccgac agcgcttggt cagcctgatc
      781 ggcggcgtcg tcggccatgc cgtggacgcg gctgagcagg gcgaagtcgt agtgacggcc
      841 gagatctggg ccagcggctc ccggctcgaa ggcaatcccg ccgagaatcc gtgggcggtg
      901 gtccaggtca agctgcccgc ttctgagcag gccgccaggc ggctagccga cgggctgcaa
      961 ccgacggcca gctcgagccg aacgccgcag ctgctgatcc cgtcgcttcc atcgcttcct
     1021 tcgatccagg ccatggccgg cggactcggc ggaaacctca ttctggagta tcccgagccc
     1081 gaccaaccct cctttcggct ctcgctccca cttctggcag cacccagagg cgagggcgac
     1141 ctccgcagca ttgtcgagta ccacttgagc ggggaaccga aatccggcga gaccttgctg
     1201 ctggtggtcg acgagccggc ccgcctggca gagatgacgg cggaactcgt cgaggagggc
     1261 tactcggtca tcccgacctc gaatgggggc gaggtattag ggctggctcg gcgcgagcag
     1321 cccgatctga ttctgctgga catgttggcc cgcgatccgg gggccctgga cgtcgcgatg
     1381 gtgctgaagc aggacagccg cacgctgaat attcctgtgt tgttcctcac ctcgaccagc
     1441 gatccacagg gggagcggct cggggcagcc agctttctcg tgcgaccaac tggcaccggt
     1501 gcgctggtct ctacgattca ggccgtgctg ggagcagggc tcagcccggc cggccgggtg
     1561 ttagtggccg agcaagatga catcgcccgc gagttcctgg tcatgatgat ccaggcccac
     1621 ggctaccggg tgaccgaggc ggccggggcc gaagaggccc tggcgcttgc cgagcatatc
     1681 ccacccgaat tggtcttggt gaattcagac atggcccacg aacgcgatta ctggctgctg
     1741 cgcggattgc gccaattggg acagggcttc gatattttcg tactggcgga agcgatcacc
     1801 gaagcagaag ggcaggaggc ggtcgaccgg ggggcttcgg gtttcagcca gaccgggcaa
     1861 ctccccgcgc tgctcgacca ggtccgcaag ggaaaaccac ggccccaaaa actcagctga
     1921 tgtaaatcgg agttccccgg ccgatccggt gcttctcgtc cggctgaacg accgggaggg
     1981 tccagcggta gagccacaag gcttcacggg tgtccatgtt gatgcagccg tggctcatcg
     2041 gtctcccgaa gtcgttgtgc caataggttc cgtggaaggc aattccggtg gcggtaaaga
     2101 aagagaccca cgggaccccg ggcagctcat aggcctccag gtccgccgtg atccgtccgt
     2161 cccccatgtg gcggcttggc attttgactt caatgaagaa ttggccggac ggagtaatcg
     2221 tcgggatccc attctcgcgg ggccggctat ccgggattcc gct
//
LOCUS       DATDPQ010000358         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1529181, whole genome shotgun sequence.
ACCESSION   DATDPQ010000358 DATDPQ010000000
VERSION     DATDPQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529181"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1111
                     /gene="fmt"
                     /locus_tag="VI520_07995"
     CDS             188..1111
                     /gene="fmt"
                     /locus_tag="VI520_07995"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLF37861.1"
                     /translation="MRVVFMGSPDFSIPTLAELARLHQVIGVVTQPDRPAGRGKQTRA
                     SVVKQFAIGHGLRVMAPESLRAPQAVGLLAGLRPELIVVAAYGQILPQAVLDLPPHGC
                     LNVHASLLPRWRGASPVQAAILNGDSETGVSIMLMNAGLDTGPILAQRATPIGAGETG
                     GSLGLRLARLGADLLIEVLPDYLAGDLGPAAQDEARATHAPRLKKANGLIDLHLPASQ
                     LARQVRAYHPWPGSYLQWNARRLLVHAARAVPMDKFAPGQALELERLPALRTGDGALV
                     LEQVQTEGKRAMDGHAFLLGSPDFLDARLLG"
     gene            1139..>2234
                     /locus_tag="VI520_08000"
     CDS             1139..>2234
                     /locus_tag="VI520_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLF37862.1"
                     /translation="MRMLGLFTTEISKVLAVPRLRPNRSNSMLLLNGRIHPRADSSAV
                     VAALLIEGGRVIWVGEPGELDIPYSNSGIVLDLGGRTVLPGLIDAHLHLEHYASRLSL
                     LDCEQPSLADCLTRVRAQAERTEEGAWILGHGWDQNLWGGYPSAADLDQGSPIHPAYL
                     TAKSLHAAWANSKALELARINASTPDPADGAIQRSADGSPTGILFEGAMRLISALIGR
                     PAIGQRLESLARAQARLLSLGLTGVHDFDGPSCFEALQLLREAERLQLRVVKNLPAEL
                     LAELQAAGFRTGFGDEWIRLGHIKVFADGALGPRTASMLSPYSGEPGNRGMLLVDHEG
                     LADIAIRAAQAGLATSVHAIGDQATHEVLGA"
BASE COUNT          380 a          717 c          701 g          436 t
ORIGIN      
        1 ggtgtccatt gcgggtttat tagtcattcc ttcctctcct gtgggtaccg ggccgccagc
       61 cggctagtcc cggcattgtc atggattgta accgcatggg ctaaagccgc aagcccatct
      121 tattgaaccc ctcgaacggc aggaggttgc tcattgttcg tcatcgctcg tatgctagca
      181 ttagagtatg cgggtggtgt tcatggggtc gccggatttt tcgattccaa ccctcgctga
      241 gctcgctcgg ctgcaccagg tgatcggagt cgtcacccaa ccggatcgac cggccggccg
      301 tggcaagcaa acccgggcct cggtggtgaa gcaatttgcg atcgggcacg gattgcgagt
      361 gatggcgcct gaaagtctgc gagcacccca ggccgtcggc cttttggccg gcctgcgacc
      421 cgagctcatc gtcgtcgcgg cctatggcca aatccttcct caggccgtgc tcgacttgcc
      481 cccgcacggg tgcctaaatg tccacgcctc cctgctgccc cgctggcgcg gtgcctcacc
      541 ggtccaagcc gccattttga acggagactc cgaaacggga gtctcgatca tgttgatgaa
      601 tgccgggctc gataccggtc ctattctcgc ccagcgcgcg accccaatcg gggccggcga
      661 gaccggcggc agtctgggcc tgcggctggc caggctcggg gcggatctgc tgatcgaggt
      721 gcttccagac tacctggctg gagatctagg accggccgcg caagatgagg cccgagccac
      781 ccacgccccg cgcctcaaga aggccaacgg tctgatcgac ctacacctcc cagcctcgca
      841 gttagcccgc caggtccgtg cttatcatcc ttggcccggg tcctatcttc aatggaacgc
      901 ccgccgcttg ctcgttcatg ccgcccgggc cgttccaatg gacaagtttg cgcccggaca
      961 ggccctcgag ctggaacgcc tcccagcgct gcggacgggc gacggcgcgc tggttctgga
     1021 gcaggtgcag acggaaggca agcgggcgat ggatggacac gccttcctgc tgggttcgcc
     1081 ggatttcctg gatgctcggc tgctcggttg acgagggttg gagaaactat gattggctat
     1141 gcgcatgctg gggttgttta ctaccgagat ctcgaaggtg ctcgccgtgc cgaggctccg
     1201 acccaaccgc agtaattcca tgctgctgct taatggccgc atccatccca gggcggactc
     1261 gtccgcggtc gtcgcggccc tgctcatcga gggcggtcgg gtgatctggg tgggcgagcc
     1321 gggcgagctc gatattccgt actccaactc agggatagtc ctcgacctag gtggcaggac
     1381 ggtgctaccg ggactgatcg atgcccatct ccacttggaa cattacgcaa gtcggctttc
     1441 cctgctcgat tgtgaacagc ccagtttggc ggactgcctg acccgggttc gggcgcaggc
     1501 cgagcgaaca gaagaagggg cctggatcct gggccatgga tgggaccaga atctttgggg
     1561 cggctacccc tccgcggcag acctggatca aggatcaccg atccaccccg cctatttgac
     1621 cgcgaaatcc ctccacgccg cttgggcgaa ttctaaggcc ctggagttgg cccggattaa
     1681 cgcctcgacc cccgacccgg ctgacggagc gatccagcgt tccgccgatg ggtcaccgac
     1741 cggcattctg tttgagggtg ccatgcgcct gatttcggcc ttgatcggcc gcccagcaat
     1801 tggccagcgg ctcgaaagct tggccagagc acaagcgcgg ctgctctccc tcggtctgac
     1861 cggggttcat gacttcgatg gacccagctg cttcgaggca ctccaactcc tgcgggaggc
     1921 cgaacgccta cagctccgag tggtcaagaa cctgccggca gagctcttag cagaacttca
     1981 agcggcaggc ttccgaaccg gattcggcga cgagtggatt cgactcggcc acatcaaggt
     2041 cttcgccgac ggggctctcg ggccccgcac cgcctcgatg ctaagcccct attccggcga
     2101 gccggggaat cgcgggatgt tgttagtcga ccacgagggg ctggccgaca tcgctattcg
     2161 tgcggcccaa gctgggttgg cgaccagcgt gcatgctatc ggagaccagg cgacccacga
     2221 agtgctgggg gcat
//
LOCUS       DATDPQ010000359         6281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2050006, whole genome shotgun sequence.
ACCESSION   DATDPQ010000359 DATDPQ010000000
VERSION     DATDPQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050006"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..745)
                     /locus_tag="VI520_08005"
     CDS             complement(20..745)
                     /locus_tag="VI520_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37863.1"
                     /translation="MPVIDPSRLAQQVAQLQAVAADPAKLTQAVAKLLDEYVDRTRRL
                     PLEDSPVPAPVQRAILHSLLGALESDPDALARTAAALWQAGPIAAKSLAAGLIGSCPG
                     GSAADLAQTWATEAVPPRVIQLLGEVGLTSWRRTHPSEFLQRAEGWLADRRRPLALYG
                     LRAAVQEAEFEDLPAVFQLLDGIGGRVRGESKRAFGLLIKALASRSANETAQFLVEQV
                     EAGVPGTAWMRALLSDRAFPSPR"
     gene            complement(773..2203)
                     /gene="proS"
                     /locus_tag="VI520_08010"
     CDS             complement(773..2203)
                     /gene="proS"
                     /locus_tag="VI520_08010"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLF37864.1"
                     /translation="MAESRLPSRAEDFSEWYNQLVLKAELADYAPVRGCMVVRPYGWT
                     LWENITAALDSRFKATGHVNAAFPLLIPMSFLQKEKDHVEGFSPQLAVVTIGGGEQLE
                     EPLVVRPTSETIIGHMYAKWIQSYRDLPVLINVWNSVVRWELRTKLFLRTTEFYWQEG
                     HTAHATAEEAQAETEQMLNVYADFAENEAAVPVIKGRKSESEKFAGAVTSYSIEAMMG
                     DTRALQSGTSHYLGDKFARAFSIQYLDRENTQQFCQTTSWGLSTRFVGAIIMVHGDDQ
                     GLVMPPRLAPHQAVLVPIYRNDQERSLVMEQVEMTRQALEGNRIRLRIDDRQEVTPGF
                     KFNDWEMRGVPVRVEIGPKDVSQGTVTLARRDQRSRQSVSQASVAEATDELLADIQRS
                     MLERAREFQRQNTHQPDDYDGFKQAVQSGWVEVWWCGDPADEIRIQQDTKATNRVILL
                     DQQAGEGRCIVCGKVARERAIFARAY"
     gene            complement(2254..3303)
                     /locus_tag="VI520_08015"
     CDS             complement(2254..3303)
                     /locus_tag="VI520_08015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HLF37865.1"
                     /translation="MEATEQWPWPFSRADLAAGLRRYLGDPTVWVEKVEPVTLSHMRP
                     SIGRVRGALVSYTQTGGEGTCRLVVKEPRGTTRTGLAGAGRREVGVYRYLASEIPVRS
                     PKLVAASEAGDWIVLEELRPERGASKWTRADYRRAVHSLTDLHDRFWGLSEDLANYPW
                     LSRPLEADFEIHVAAAANSLERIADHGRLASWPERVHTMAHLILHADQVVAPLRQEPA
                     TLLHGDYWPGNIAVIDENTQVVYDWQMAGVGPPMLDVVAFVLKSEWWFGGLPLGRGLI
                     AQEYRQLLAKISGQRWADERWDELWDHALMWRFLQEWLDLLAISPSTLLEARSEQLEE
                     LWFEPLAKAVARRLM"
     gene            complement(3419..4645)
                     /gene="metK"
                     /locus_tag="VI520_08020"
     CDS             complement(3419..4645)
                     /gene="metK"
                     /locus_tag="VI520_08020"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLF37866.1"
                     /translation="MTTSFMASPRLMFTSESVTEGHPDKLCDQLSDAVLDACLSQDPL
                     SRVACEVASKTGFVMFLGEITSQAQINFDELARKVILGVGYDSGAKGFDGRTCAVQVA
                     IASQSPDIAQGVNRALEVRTSEVSDEEVETIGAGDQGMMFGYACTETATLMPMPIYLA
                     HRLTRRMAEVRRSGELPWMRPDGKSQVTVQYEFGRPIRVDTVLISAQHAPDVSLSEIE
                     ARIKEQVILEVLPEHMVDRDMKVYVNPTGRFVTGGPLGDSGLTGRKIIVDTYGGMGRH
                     GGGCFSGKDATKVDRSGAYAARWVAKHIVAAGLADRCEIQIAYAIGIAHPLSINVETF
                     GTGQLDDAKIVEIIQEYFDLRPGAIIRDLGLRRPIFQPTASYGHFGRDDLDLPWERLG
                     RLESIQAASGVLVEHA"
     gene            4815..>6281
                     /locus_tag="VI520_08025"
     CDS             4815..>6281
                     /locus_tag="VI520_08025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLF37867.1"
                     /translation="MRGAAALMLCFLLFAPIRSGSPDRYPGAAGSGDRAAEPQLDNEF
                     DRGAGGREVRTGLFSFEALTELAGLRLLPSEVEAENAWLPAGVIAGRIWNGPVYRLEA
                     EAGLISDGQFVWGPNVGPFNIGAFLRSRKSPLAPYAADLELWASYSSVNPKVLLTVLE
                     IRDGWVTSLPEDTPALEVSQRIEATAMQLAATFYEHLHTWGARRPSLSIAPAGGPAIW
                     YADGSVAILDASTSSGTAAVAAVLAADTYPEGFSVQGAAQPTSFRLVFGALFPGTDLL
                     SEANQINPLSAPPASLLQLPFPLGATWTASGPHSWNGGNYPPPFSSLDFFTGGGTCGA
                     PPNLYAVAAASGSVYRPFGYSCWLEIDHGAGWVTSYYHLRNLYSGAPLGRAGKVGTIA
                     CELCAGGFSTGPHVHFTLKFNGAYASLDGVKLSGWTVRAGAVPYTSGSFERDGASRPA
                     YSSINNDYQSYYFPGFSSLRFYGSGAGDVDRLKIRLEDL"
BASE COUNT         1106 a         2068 c         1904 g         1203 t
ORIGIN      
        1 gcattatacc atcccgaacc tagcgaggcg aagggaaggc ccggtccgag agcagggccc
       61 tcatccaggc cgtgcccgga actccggcct ccacttgttc aaccaggaac tgggcagtct
      121 cattcgccga acgacttgcc agggccttga tcaacaggcc aaaggcccgc ttcgactctc
      181 cgcgcacccg ccctccgatt ccatccaaga gttgaaacac agccggaagg tcctcgaact
      241 ccgcctcttg aaccgcagcc ctcagtccgt acaatgccaa gggccgacgg cggtcggcga
      301 gccagccttc ggcccgctgc aggaactcgc tggggtgcgt tcgccgccag ctggttagtc
      361 cgacttcgcc aagcagttgg atcacccgcg gcgggacggc ctcggtcgcc caggtttggg
      421 ccaggtctgc cgcggaaccg cctgggcatg aaccgatcag accggctgcc agcgactttg
      481 ccgcgatggg gccagcctgc cacagggccg ccgcggtccg cgccaaagcg tcagggtcac
      541 tctccagtgc gccgagcagg gaatgcaaaa tggcccgctg gaccggggcc ggaactgggg
      601 agtcctctag cggcaggcga cgggtccggt caacgtactc atccagcagc ttggctacgg
      661 cttgagtcaa cttggccggg tcggctgcta ccgcctgcag ttgtgcgacc tgttgcgcca
      721 gccggctggg gtcaattact ggcatgtggg aacgccgggg gctgcggctg agtcagtagg
      781 cccgggcgaa gatggcccgc tcccgggcaa ccttgccgca gacaatgcag cggccttccc
      841 cggcttgttg atccagcaga atgacccgat tggtggcttt ggtgtcctgc tggattcgga
      901 tttcatccgc cggatcgccg caccaccaca cctcgaccca accggattga accgcctgtt
      961 tgaagccatc gtagtcatcc ggctggtgtg tgttttgccg ctgaaactcg cgggcccgct
     1021 cgagcatcga acgctggata tcggccagca gttcgtccgt ggcctccgcc acgctcgcct
     1081 ggctcacgga ctgccggctg cgttggtcgc gtcgggcgag ggtgaccgtc ccctggctga
     1141 cgtctttcgg accgatctcg acccggaccg gaactccccg catctcccag tcattgaact
     1201 tgaatccggg tgtcacttcc tggcgatcgt cgatgcggag ccggatccgg ttgccttcta
     1261 gcgcctgacg ggtcatttcc acttgttcca tgaccaggct gcgctcttga tcgttcctgt
     1321 agatcggcac cagcactgcc tgatgaggcg ccaatcgggg cggcatcacc agtccctggt
     1381 cgtctccgtg gaccatgatg atcgcaccca caaaccgggt cgaaagcccc cacgaagtgg
     1441 tctggcagaa ctgctgggtg ttctctcgat cgagatactg aatggagaag gccctggcga
     1501 atttgtcgcc aagataatgg gaggtcccag actgcagggc ccgggtgtct cccatcattg
     1561 cctcaatgct gtacgaggtc accgcccccg cgaatttctc gctctcgctc ttacgcccct
     1621 tgatcaccgg caccgcggcc tcgttctccg caaaatcggc gtacacgttc aacatctgct
     1681 cggtctcggc ctgggcctct tcggcggtcg cgtgggccgt gtgtccctcc tgccagtaga
     1741 actcggtggt gcgcagaaac agcttggtgc gcagctccca gcgcacgaca ctgttccaga
     1801 cgttgatcag caccggaagg tcccggtacg actggatcca cttggcatac atgtgcccga
     1861 tgatcgtctc cgaggtcgga cggacgacca gcggttcttc cagctgctct cccccaccga
     1921 tagtgacaac tgccaactgc ggagaaaaac cctccacatg gtctttctct ttctgtagga
     1981 agctcattgg gatcaacagc gggaaggcgg cgttgacgtg cccggtcgcc ttgaatcggc
     2041 tatcgagcgc ggcggtaatg ttctcccaga gcgtccaccc gtagggtcgc acgaccatgc
     2101 agccccgcac cggtgcgtag tccgccagct cggccttcag aaccagctgg ttgtaccact
     2161 ctgagaagtc ctctgcccgg gagggcaaac gcgattcagc catcgatccg cctcttccta
     2221 tctaccgcct gcgaagccgt ggccggacct gggtcacatc agccggcggg ccacagcctt
     2281 tgccaacggt tcgaaccaca attcttccag ctgctccgac ctggcctcta acaaggtgct
     2341 gggtgagatc gccagcaggt ccagccactc ctgaagaaag cgccacatca gggcgtgatc
     2401 ccacaactcg tcccaacgct catcggccca gcgctgccct gaaatcttgg cgagcaattg
     2461 tcggtactcc tgggcgatga ggccgcgccc gagcggcaat ccgccgaacc accactcact
     2521 cttgagcacg aaggctacga catccagcat cggcggtcca acccccgcca tctgccagtc
     2581 gtagacgacc tgggtgttct cgtcaatcac ggcgatattg cctggccagt agtcaccgtg
     2641 cagcagagtg gccggctcct gcctcagcgg tgcgaccacc tggtccgcgt gcaggatcag
     2701 gtgagccatc gtgtgcaccc gctccggcca gctagccaac cgtccgtgat cggcaatccg
     2761 ctccagcgag ttagccgcgg ctgcaacgtg gatttcgaaa tccgcctcga gcggccggct
     2821 gagccagggg taatttgcca ggtcttctga cagtccccag aagcggtcgt gcaggtcggt
     2881 caaggagtgg accgccctcc ggtagtcggc tcgggtccat ttcgaggccc cccgctccgg
     2941 tctcagctct tccagcacaa tccaatcacc ggcctcggag gctgctacca gtttcggaga
     3001 gcgaaccggg atctcggagg ccagatagcg atagaccccg acctctcgcc gtccggctcc
     3061 ggccaatccg gtgcgggtcg ttccgcgggg ttccttgacg accagccggc aagttccctc
     3121 gccgcctgtc tgagtgtagc tgaccagggc acctcgcacc cggccaatcg aagggcgcat
     3181 gtggctcaag gtaaccggct cgactttctc cacccacacg gtcggatccc ctaggtaacg
     3241 ccgaagaccg gccgccaagt cggcccggct gaatggccag ggccactgtt ccgtcgcctc
     3301 catttccgat tgagctccgc ccgatgactc gacttgtgac aaatttaatc ctgctccgag
     3361 aaagaaaacg gcttccctgc actctgggaa gccgtgggaa gtcccctcgg gcctccagct
     3421 acgcgtgctc taccagcacc cctgaggcgg cctgaatgct ttccaaccga cccagccgct
     3481 cccagggtag atccagatcg tctcgtccaa agtgtccata ggaggcggtt ggctggaaga
     3541 ttgggcggcg caatcccagg tcgcggatga tcgcgccagg tctcaaatca aaatactcct
     3601 ggatgatctc gacgatcttc gcgtcgtcca gctgcccggt gccgaacgtc tcgacattga
     3661 tcgagagcgg atgagcaatt ccgatcgcgt aggcaatttg gatctcgcag cggtcggcca
     3721 gcccggcggc gacgatgtgt ttggccaccc agcgggcggc gtaggccccc gaccgatcca
     3781 ccttggtggc gtcctttccg ctgaagcagc ccccgccgtg gcggcccatc ccgccgtagg
     3841 tgtccacgat aatcttgcgg ccggtaagtc cggagtcgcc gagcgggccg ccggtgacga
     3901 accggccggt cgggttgaca tacaccttca tgtctcgatc gaccatgtgc tccggcagta
     3961 cctccaggat gacctgctcc ttgatccttg cctcaatctc cgatagcgag acatccggag
     4021 catgttgagc gctgatcagc acggtgtcga ctcggatcgg gcgaccgaac tcgtactgga
     4081 ctgtgacctg ggactttcca tccggccgca tccatggcag ctcgccgctg cggcggacct
     4141 ccgccatccg acgggtcagc cggtgggcca ggtagatcgg catcggcatc agggtcgcgg
     4201 tctcggtgca ggcatagcca aacatcatgc cttgatcgcc ggcgccgatg gtctcaactt
     4261 cctcgtcact gacttcgctc gtgcgcacct cgagggctcg attcacacct tgggcaatgt
     4321 ccgggctctg cgatgcgatc gcaacttgca ccgcacaggt gcggccgtcg aagcccttcg
     4381 caccgctgtc ataaccgaca cccaggatga ccttgcgggc cagctcgtca aagttgatct
     4441 gagcctggct ggttatctcg cccaggaaca taacgaagcc ggtcttggag gccacttcgc
     4501 aggcgaccct ggacagcgga tcttgggaga ggcaggcgtc gagcactgcg tcggagagct
     4561 gatcgcagag cttgtccgga tggccctcgg tcaccgactc cgaagtaaac atcagcctcg
     4621 gggaggccat gaagctagtt gtcatctgca cttctccgat tcaaggaaat ccccttccgc
     4681 gcgggaaggg gcgaggggca aagtttttcg cagtatggtg cggcatgata ccacagcttg
     4741 ggtcagaccg cggcccattt cgtgcacctg ggggcagaac gaaggtgacc gaagctgaga
     4801 agggccggag cagaatgcgg ggcgcagctg cactgatgct gtgcttcctg ctgttcgccc
     4861 cgatccggtc cggcagcccg gatcggtacc cgggagccgc agggtcgggc gatcgagccg
     4921 ccgagccgca gctcgacaat gagttcgata ggggagctgg cggccgggaa gtgcggaccg
     4981 gtctgttctc cttcgaagcg ctgaccgagc tcgccggtct gcggctgctg cccagtgagg
     5041 tagaggcaga aaatgcctgg ctcccggcgg gggtgatcgc cggcaggatc tggaatgggc
     5101 cggtctatcg gctcgaggcc gaagcgggtc ttatttccga cggtcagttt gtttggggac
     5161 cgaacgtcgg gccattcaat atcggagcct tcctgcggag tcgaaagtcc ccgctggccc
     5221 cctacgccgc cgatctcgag ttgtgggcca gctactccag tgtcaatcca aaggtattgc
     5281 tgacggtgct cgaaattcgg gatggctggg tgaccagcct cccggaagat actccggccc
     5341 tggaggtcag ccaacggatc gaagcgaccg caatgcagtt ggcggcaacc ttctacgaac
     5401 acctgcatac ctggggagcc cgcagacctt ccctctccat tgcaccggct ggcgggcccg
     5461 ccatctggta cgccgacggg tcggttgcga ttctggatgc ctccacgtct tccgggaccg
     5521 ccgcggtggc cgctgtgctg gccgccgaca cgtaccctga aggcttctcg gttcagggcg
     5581 ccgctcagcc aacctccttc cgacttgtgt ttggagccct cttcccaggg accgatctgc
     5641 tgagcgaggc caaccagatc aacccgctca gtgccccgcc agccagcttg cttcaactcc
     5701 ccttcccgct cggtgccacc tggaccgcca gcgggccgca cagctggaac ggaggtaatt
     5761 acccaccccc gttcagttcg ctggacttct tcaccggagg gggtacctgc ggcgcgccgc
     5821 ctaatctgta cgcggttgct gcggcatcgg gttcggtata ccgtccgttc ggctacagct
     5881 gctggctgga gattgaccat ggagccggtt gggtgaccag ctactatcac ttgcggaacc
     5941 tctacagcgg cgccccgctt gggcgagcgg gcaaagtcgg aaccattgcc tgcgagttgt
     6001 gcgccggcgg attctccacc ggcccgcacg ttcatttcac tctgaaattc aacggggcct
     6061 atgccagcct ggatggagta aagctatcgg gttggaccgt gcgcgcgggg gcggtgccgt
     6121 acaccagcgg ctctttcgaa cgggacgggg cgtcgcgtcc ggcctacagc tcgatcaaca
     6181 acgattacca gtcctactac ttcccgggtt tcagctcgct tcgtttctac gggtctgggg
     6241 ccggagatgt ggatcggctg aagattcgtc tggaagacct t
//
LOCUS       DATDPQ010000360         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2318969, whole genome shotgun sequence.
ACCESSION   DATDPQ010000360 DATDPQ010000000
VERSION     DATDPQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318969"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VI520_08030"
     CDS             <1..714
                     /locus_tag="VI520_08030"
                     /inference="COORDINATES: protein motif:HMM:NF023746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLF37868.1"
                     /translation="AVVIATVTMPFGFEMSRRAKNAVEGVKRLQPHTHTLITVPNDRL
                     LEAVPRNASLEVAFRFADDVLRQGIQGIVELVTKPGLINVDFAHVRNLMQQGGGALMA
                     IGLGEGQDKASMAVQQALHHPLLEVNSLEFATGALLHFTGGDDLSLAEVGTAVEKLRE
                     SLPLDLDVVLGVTPEPTMEGRVQVILVVTGIGGRPIQSFVTDQPEPFSLVPDNLDVPA
                     FLRKRSMGVGSPRLHSETS"
     gene            716..949
                     /locus_tag="VI520_08035"
     CDS             716..949
                     /locus_tag="VI520_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37869.1"
                     /translation="MNRETVGKITRTVVGQFPELSGVRPAVRRQAPPGNSPALFLLTY
                     KGAARLPNGKSLQRVVRVVADEEGHVIRISTSR"
     gene            954..1154
                     /locus_tag="VI520_08040"
     CDS             954..1154
                     /locus_tag="VI520_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37870.1"
                     /translation="MNQLKYRPIVVLNLLLLKTTVFVAEWALKHREAVRLGRAYAPVV
                     LGSFAAYFAGRVVGQAIINGLV"
     gene            1313..2014
                     /locus_tag="VI520_08045"
     CDS             1313..2014
                     /locus_tag="VI520_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37871.1"
                     /translation="MVVIVLALFFLAGLVTVLLGSQSPGVSSASPQDGSGETVLIVGV
                     DDLAAAQPKLVAVWIATFSVENRDVLFYGQPIDAPVCGNTPTTLQELFLWQPQSGLSP
                     AFLEAFGATGPDAVVVADETAFIALVDELHGLDLNGAKLEGDQVVAVQRTLYQNPAGL
                     LDTQEQFLIALARRATDAASQVDLTRLTNQIPGHAYTSVPPQDLITLYLRLLPLGPSS
                     ILVFASQNSAVPCPG"
     gene            complement(2049..2133)
                     /locus_tag="VI520_08050"
     tRNA            complement(2049..2133)
                     /locus_tag="VI520_08050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2097..2099),aa:Leu,seq:gag)
     gene            2337..>2524
                     /locus_tag="VI520_08055"
     CDS             2337..>2524
                     /locus_tag="VI520_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37872.1"
                     /translation="MRIVLDAMGSDSAPGPELAAAAQAYSRWGEPLTLTGPRRLLENR
                     ADSSAFEVADAPEILEMT"
BASE COUNT          479 a          779 c          787 g          479 t
ORIGIN      
        1 gcggtggtca tcgcaacggt gaccatgccc ttcggtttcg aaatgagccg gcgagccaag
       61 aacgcggtcg aaggagtcaa gcgcctgcag cctcacaccc acactttgat tacagtgccg
      121 aatgaccggc tgctggaggc cgttccccgg aatgccagtc tcgaggtcgc ctttcgtttc
      181 gccgatgacg tgctgcggca agggattcaa ggaatcgtgg agctcgtcac caaacccggc
      241 ttgatcaatg tggattttgc ccatgtgcgc aacctgatgc agcaaggggg cggcgccctg
      301 atggcgatcg ggctcggaga aggtcaagac aaggcctcca tggctgtgca acaggcgttg
      361 catcatcccc tgctggaggt caacagtctg gaatttgcaa caggggccct gctgcacttc
      421 accgggggcg atgacctgag cttggcagag gtcggcacgg cggtcgagaa actgcgcgag
      481 agcctgccgc tcgacctgga tgtggtcctg ggagttacgc cggagccgac gatggaagga
      541 cgggttcagg ttatcctggt ggtcaccgga atcggcggcc gcccgatcca gagcttcgtc
      601 acagatcaac ccgagccatt ttcgcttgtc ccggataatc tcgacgtacc ggccttccta
      661 cgcaaacggt ccatgggggt aggcagccca aggctgcatt cagaaacgag ctagcgtgaa
      721 tcgggagacg gtcggcaaga tcacccgcac ggtcgtgggg cagttccctg aactgagtgg
      781 ggttcggccg gcggtccggc gccaggcgcc cccgggaaat tcgccggccc tattcctgct
      841 cacctacaaa ggggcggccc gcctgccgaa cggcaagtcc ttgcagagag ttgtgcgggt
      901 cgtggccgat gaagagggcc acgtgatccg aatcagcaca tcgaggtgaa accatgaacc
      961 agctcaagta tcgtccgatc gtggtgctca acctgctcct gctcaagacc accgtctttg
     1021 tggcagagtg ggcgctcaag caccgcgagg cggtcaggct cggccgcgcc tacgcaccgg
     1081 ttgtgcttgg tagtttcgcg gcctacttcg ccggccgggt ggttgggcag gcgatcatca
     1141 acgggctcgt atgaagttgt tgcagaccga gtgagtgccg gccattcggc cagaatcaac
     1201 ttcgatagac cactgaagtg gcgccagcct gcgcccggag tacaatccgg gcgcatttgt
     1261 tcgaatcaaa ccccaatcct cttcgagaat gacccagcag cgccggccaa tagtggtcgt
     1321 cattgtcctc gcgttgttct tccttgccgg gctggtcacc gtactcctgg gcagccagag
     1381 tcccggagtg agcagtgcga gcccacagga cggttcaggc gaaacggtcc tgattgtggg
     1441 ggtggacgac ctggccgcgg ctcaacccaa gctggtcgcg gtctggatag cgacattcag
     1501 tgtggagaat cgggacgttc tgttctacgg ccagccgatc gacgctccgg tctgcggcaa
     1561 tacccccacc accttacagg agttgtttct ttggcagccc cagagcggcc tgagccccgc
     1621 tttcctggag gcctttggag ccactggccc ggatgcggtg gtggtcgccg atgaaaccgc
     1681 cttcatcgcg ttggtcgacg aactgcatgg attggatttg aatggggcga aactcgaagg
     1741 cgatcaggtg gtcgcagtgc agcggacact gtatcagaac ccggcggggc tcctggacac
     1801 ccaagaacag ttcttgattg ccctcgcccg ccgggcgacg gatgccgcca gccaggtcga
     1861 ccttacccgc ctcaccaatc aaatccccgg ccatgcctac acctccgtac cgccgcagga
     1921 tctcattacg ctgtatttgc gattgctccc gctcgggccg tcctcgatcc tggtgttcgc
     1981 ttcccaaaat tcggccgtcc cctgtccggg ttgagacaaa atcgggttgc ctttcggcaa
     2041 cccgcggatg gtgcgctggg tgggacttga acccacacgc cttgcggcac atggccctca
     2101 accatgcctg tctaccaatt ccagcaccag cgcaagactg atcgaaagcg agcaggccat
     2161 cgatatcgcc gctgcgcgcc gacgatcctc gcccgatttg cggtattata gcgaacggcc
     2221 gggcggagtc aacggaacca gccgaacagc cccgaccagg cacttacccc cagagtgttc
     2281 gactgcaaat ggcggcgaag ctgatctcgg cggcccacaa gctcgttggc taactgatgc
     2341 ggatcgtctt agatgcgatg ggaagcgatt ccgccccggg tccggaactg gcggccgcgg
     2401 ctcaggccta cagccgatgg ggtgagcccc tcacgctgac cggcccccgc cggctgttgg
     2461 agaatcgggc cgactcctcg gctttcgagg tggccgatgc tcccgagatt ctggaaatga
     2521 ccga
//
LOCUS       DATDPQ010000361         4370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2051832, whole genome shotgun sequence.
ACCESSION   DATDPQ010000361 DATDPQ010000000
VERSION     DATDPQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051832"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VI520_08060"
     CDS             <1..490
                     /locus_tag="VI520_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37873.1"
                     /translation="VLVPAAAAGGWAIATLDQLPGEVHAGETVSLGFMVRQHGKTPTN
                     EVEPYVQAYNSESGESVRANAVQEGEVGHFVVEIVFPAAGTWEWSIVPAPFEGTQLEP
                     LSVQPATLTQSAASPQQSVAFDANNLRVLLRWSGIALLLGAGVLAALGQRSSRRAAKE
                     RA"
     gene            521..904
                     /locus_tag="VI520_08065"
     CDS             521..904
                     /locus_tag="VI520_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLF37874.1"
                     /translation="MKRGVLPLALAGAGLLILSLVPSSVDPGGGVEPPPAVSAAATTE
                     PAAYGRALFAAKGCVGCHTHNDVNPGSGIAVGPDLTYRALDPDFLRSWLKDPPAVRPG
                     TQMPNLRLSAEEIEALIAFLVSEGS"
     gene            complement(934..1875)
                     /gene="rsgA"
                     /locus_tag="VI520_08070"
     CDS             complement(934..1875)
                     /gene="rsgA"
                     /locus_tag="VI520_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HLF37875.1"
                     /translation="MPGSAKPVRAVEPLHGLVLRASAGFYEVETTQGVITAKLRGRLK
                     KSARRAGAAAIGDQVRLSLYEDGSASIEEILPRRRALSRRAPGREAEQILVANPDQSV
                     FVFACADPDPNFRMLDRLLVVAERESLPAVICANKLDLVEPRSAHEEFGVYEQIGYRV
                     LYTSATTGKGVGDLRKELSGKLSVLAGPSGVGKSSLLNLVQPGLGLRTEAVSLSTGKG
                     THTTVVRELLPLKGGGYVADTPGLKAFALWDIEPDELDAYFREIGPLVKDCEFSDCTH
                     EHEPGCAVRAAVEAGKIDVNRYESYLRMRAGETFLER"
     gene            complement(1905..2867)
                     /locus_tag="VI520_08075"
     CDS             complement(1905..2867)
                     /locus_tag="VI520_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37876.1"
                     /translation="MASEIPRPTRVAPSEPPEPSSTPPPLALLAEANVDQPFALAWSP
                     GGDLIVVTTAAGLHLIDAADPRQQRLIPAEKPLEFLAFSPEGDLLATTEAGTRFQVWA
                     LPEGELVDQQEITINRHFGVFFDDHGALYVATQYGGESLGIQTFTGREVTGGLAVGDG
                     ADWVDDIAIAPNGVLAAFNSFEGIDIWSFDTQARAAVLEGERSAALRFSPDSTLLAAA
                     HWDGIARLWSLDSGAVLQSFIWRETDAGGPQRTGLDFGPDGRLLAVAGKEGRILVWDT
                     ESQTIADTFPIALRTFNSIGFRPDGTKLASIGEDGLLQIWAVGP"
     gene            complement(2978..3409)
                     /locus_tag="VI520_08080"
     CDS             complement(2978..3409)
                     /locus_tag="VI520_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37877.1"
                     /translation="MADRADNLESEGRATFEISLDEVPPSLILAVHPDRLWPPNYQRV
                     PVRITIDATDEDSGLAGITIRAEDEYDKHEPAIEPMVFAGEPSAHVEIVVELIASRMG
                     SDKNGRQYRIIAEVTDMAGNSAVAMAVVMVPHDQGKVMERR"
     gene            complement(3599..>4370)
                     /locus_tag="VI520_08085"
     CDS             complement(3599..>4370)
                     /locus_tag="VI520_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLF37878.1"
                     /translation="LERSCNTYFFHIGLDLFNRGLESALPDMARGFGLGQPTGIEIGE
                     ASGLLPDPENKLTLYGDPWNRTDNVMLSIGQSYLQATPLQVARFVAAIGNGGTLYRPQ
                     IVASIQNAEGEVLSEFSPVVQSSLPVSPENLAAIQHAMVGVIRDPRATAYRPFLGIPD
                     INIAGKTGTAETGEFSDTHAWFAGYTFEERSDRPDIAVVVVLEYQGQGSEWGAPVFKR
                     VVEAYFKGGPISRYPWEARIRVPATPEPVEEEGAGTDG"
BASE COUNT          784 a         1442 c         1339 g          805 t
ORIGIN      
        1 cgtgctcgtt ccggccgcgg cggccggcgg atgggccatc gccacgctgg atcaactgcc
       61 gggcgaagtc cacgctggtg agaccgtaag cctggggttc atggtccgcc agcacggaaa
      121 gaccccgacc aacgaggttg aaccgtatgt gcaggcctac aactccgagt cgggtgagag
      181 cgtgcgggcg aacgcggttc aggaaggcga agtggggcac ttcgtcgtcg agatcgtctt
      241 cccggcggcg ggtacctggg aatggtcaat cgttccggcg ccattcgagg ggacccaact
      301 cgagccgttg agtgtgcagc cggccacgct gactcaatcg gcggccagcc ctcagcaatc
      361 tgtcgccttc gatgctaaca acctgcgggt tctactccgt tggtcgggga tcgccctgct
      421 gctgggcgcg ggagtcctgg ctgcgctcgg ccagcgcagc tcgcgccggg cggccaaaga
      481 gcgggcctga accccctgcg gcggattggg taacgctgtg atgaagcgag gggtcctgcc
      541 gttggcgctg gcgggtgcag ggttgctgat cttgtccctc gtcccaagct ctgtagaccc
      601 gggtggcggc gttgagccgc cacccgcggt ctcggccgcg gcaaccaccg aaccggcggc
      661 gtacggcagg gccctcttcg cggccaaagg ctgcgtcggt tgccataccc acaatgacgt
      721 aaaccctggc tccggcatcg cagtgggacc cgatctgaca taccgagccc tcgatccgga
      781 cttcctgcgc agctggctca aggatccacc tgcggtaagg ccgggaaccc agatgccgaa
      841 cctccgcctc tctgccgagg agattgaagc cctgattgcc ttcctggtga gcgaaggcag
      901 ctaggcgccg gcgctgccgg ccgccgcgcg gagttagcgc tctaggaagg tctcgcccgc
      961 tcgcattcgc aggtaagact catagcgatt gacgtcgatc tttcctgcct ccaccgcagc
     1021 ccgcaccgcg cagcccggct cgtgctcgtg ggtgcagtcg ctgaattcgc aatccttcac
     1081 taagggcccg atctctcgga agtaggcgtc cagctcgtcc ggctcgatat cccacaacgc
     1141 gaacgctttg aggcccggag tgtcggccac atatccgccg cccttcagcg ggagcagctc
     1201 gcggactacg gtcgtgtggg tccctttgcc ggtcgacagg ctcacggcct ccgtgcgcaa
     1261 gccgagacct ggttgaacca ggttgagcaa gctggacttg cccaccccgg agggtccggc
     1321 caacaccgag agcttcccac tcagctcttt ccgcaaatct cccacccctt ttccagtggt
     1381 cgcggaggta tagagcaccc ggtacccaat ctgctcataa accccaaatt cctcgtgcgc
     1441 gctgcgcggc tccaccagat cgagcttgtt ggcgcagata accgccggga gggactcccg
     1501 ctcggctacg accaacagcc ggtcaagcat gcggaaattg gggtcgggat cggcgcaggc
     1561 gaatacgaag accgattgat ccgggttagc gaccaggatc tgttcggcct cccggccggg
     1621 ggcccgacgc gagagcgccc ttcggcgggg cagaatctct tcgatcgagg ccgagccgtc
     1681 ctcgtacagg ctgagcctga cctgatcccc gatcgctgcc gctccggccc ggcgggccga
     1741 ctttttcaaa cggccgcgca gcttcgcggt aatcacgccc tgagtcgttt cgacctcata
     1801 gaagccggcg ctggcccgca ggaccaggcc gtgcaatggc tcaactgcac gcacgggttt
     1861 tgcagaaccc ggcatgggcg atttctcctt gctcacctgt cgcttcaggg cccaaccgcc
     1921 cagatctgaa gcagtccatc ctcgccgatc gaggcaagct tcgtcccgtc cggcctgaag
     1981 cctatgctgt tgaaagtcct cagcgcaatg gggaaggtgt cggcgatggt ttgggactcc
     2041 gtatcccaaa ccaggatacg cccctccttg cccgccaccg ccaacagccg gccatcgggg
     2101 ccgaaatcga ggccggttcg ctgcggaccg cccgcatcgg tctcacgcca tatgaacgac
     2161 tgcagaacgg cgcccgagtc tagcgaccac aacctggcta tcccatccca atgggctgca
     2221 gccaggaggg tgctgtccgg gctgaaccgc agcgcggccg atctctcgcc ctccaggacg
     2281 gccgcacggg cctgggtgtc gaagctccaa atgtctatgc cctcaaagct gttgaatgca
     2341 gccaacacgc cgtttggcgc gatggcgatg tcatccaccc agtctgcgcc atcgccaacc
     2401 gcaagcccgc cggtgacctc cctgcctgta aaggtctgga tccccaggct ctcgccgcca
     2461 tactgcgtcg ccacgtacaa cgcaccgtgg tcgtcgaaga acactccgaa atggcgattg
     2521 atggtgatct cttgttggtc tacgagttcg ccttctggca gagcccatac ttggaaccgg
     2581 gtccctgcct ccgtggtggc gagcaggtct ccctcgggtg aaaaggccag gaactctaag
     2641 ggcttctcgg cgggaatcag tcgctgttgc cttggatccg ccgcgtcgat caggtggagt
     2701 ccagccgcgg tcgtcaccac gatcaaatcg ccgcccgggg accacgccag cgcgaagggt
     2761 tgatcgacgt tcgcctctgc caagagcgca aggggtggcg gggtcgatga gggctcaggc
     2821 ggctcggagg gagccacccg ggtggggcgt ggaatctccg acgccatgga agtggctatt
     2881 aggggagtcg ctcccgaacc aggtccccca cctgtgcatc cagctatgaa gaggagtcct
     2941 gcaagcccag ccgagagacg cgtgcctcga cccattctta tctccgttcc atgactttgc
     3001 cttgatcgtg agggaccatg accacggcca ttgccacggc cgaattcccc gccatgtcgg
     3061 tcacttcagc gatgatccga tattgccttc cgttcttatc ggatcccata cgggaggcga
     3121 tcagctccac cacgatctcc acgtgcgcag agggctcacc cgcgaaaacc atcggttcga
     3181 tcgcgggttc gtgcttatcg tattcgtcct ccgcccgaat cgtaatcccc gccaagccgc
     3241 tgtcttcgtc tgtggcatcg atcgtaatcc tcacaggaac ccgctgatag ttcggcggcc
     3301 aaagtctgtc cggatgaacg gccaggatga ggctcggcgg gacttcatcc agcgagatct
     3361 cgaacgtagc ccgcccctcg gactcgaggt tgtcagcccg gtccgccatc ttcgcgcggc
     3421 ccgccctctc ccaattgtga tcaccttcac atatggcagc tggctaagca ggacgactgc
     3481 aagattcaag ctccagcagg caggcctgcc agctggattg tatagtcatg aaagctgcga
     3541 ataagacaga ggtaaagact gaaatcaaca ggccggtgaa gagacgctga ctggcggatc
     3601 agccgtcggt ccccgctccc tcctcttcca ccggctccgg cgtggcagga acgcggatgc
     3661 gagcttccca aggatagcgg ctgatcggac cccccttgaa gtaggcttcg acgactcgct
     3721 tgaataccgg cgcgccccac tcggaccctt ggccctggta ctccagcaca acgaccaccg
     3781 cgatgtccgg ccggtcggaa cgctcttcga aggtgtaccc cgcaaaccag gcatgggtat
     3841 cgctgaactc cccggtctcg gcggtgccgg tcttgcccgc aatattgatg tcgggtattc
     3901 cgaggaacgg ccggtaggcc gtagcccgcg ggtcccggat cactccgacc atcgcgtgct
     3961 ggatggcagc caggttctcc gggctgaccg gtagactact ttgaaccacc ggggagaact
     4021 cgctcagcac ctctccctcg gcattctgga tgctggccac gatttgcggc cgatagaggg
     4081 tgcccccatt gccgatcgcg gctacaaacc gggccacctg aagtggggtg gcttgcaggt
     4141 agctctgtcc gatcgagagc atcacgttgt cagtccggtt ccaagggtca ccatagaggg
     4201 tgagcttgtt ttccggatcc ggcagcagtc cgctggcctc tccgatctca attccggtgg
     4261 gctgaccgag tccgaagccc ctggccatat ccgggagggc gctctccagg ccgcggttga
     4321 agaggtcgag cccgatgtgg aagaagtacg tattgcagga tcgctccagg
//
LOCUS       DATDPQ010000362         5084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3902398, whole genome shotgun sequence.
ACCESSION   DATDPQ010000362 DATDPQ010000000
VERSION     DATDPQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902398"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..406)
                     /locus_tag="VI520_08090"
     CDS             complement(65..406)
                     /locus_tag="VI520_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLF37879.1"
                     /translation="MPSKASSHICPISSIARLLGRKWTLELLYFLRSNKRFCDLQEVT
                     HDISPTTLTRRLRELEKAGLLKRTVIPTFPPTVEYELSNKGEELRPMIDLLTLWSRRW
                     IRPEREGRLLG"
     gene            591..884
                     /locus_tag="VI520_08095"
     CDS             591..884
                     /locus_tag="VI520_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLF37880.1"
                     /translation="MPIYTYRCGNCGVQFERKQGFEDPLLRKCPECGETTLHKLFTPV
                     GIHFKGPGFYSTDHGSGSRSNGSAKSEPKKEDSGSAKPSEPSPKPDKKPTSED"
     gene            complement(952..1254)
                     /locus_tag="VI520_08100"
     CDS             complement(952..1254)
                     /locus_tag="VI520_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37881.1"
                     /translation="MPVKLIMTWDIMPGREQEYFEFVVREFVPGMQKIGIQPTEAWFT
                     QYGDQPQILTGGIADTLALMQQALTSDDWQQLHGHLGEYVTNFSMKVVKASGGFQI"
     gene            complement(1270..1911)
                     /locus_tag="VI520_08105"
     CDS             complement(1270..1911)
                     /locus_tag="VI520_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLF37882.1"
                     /translation="MEIVWYGLSCFRMKERGLATVVTDPYDTALGLPALKLTADVVTI
                     SHDSPGHNNFGGVKGERLRVTGPGEYEVGGVFITGISMAAKGQRKNGKVPNTLYVFEF
                     EGLTVAHLGDLAYVPTQAQIEDLGPVDIALVPVGGGEGLTPAEAAEVISLIEPSLVVP
                     MQFKTGGEKIKLGQVAGFLSEMGIGKQEPQERLTVTKSGLSDQTQVVVLQRSA"
     gene            2015..2800
                     /locus_tag="VI520_08110"
     CDS             2015..2800
                     /locus_tag="VI520_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37883.1"
                     /translation="MGALRYGLKPHLLGGGQVFPIDARSFRLEIPTLPRGYADAQVDD
                     CKGRRRSQFAWAPPASLTLRARASHPAPPGTMGFGFWNDPFGLSLGGAGGVQKLPAAP
                     QAVWFFYGAPPNDMNFVSGGRGDGWKAASLRSPAVPAALLVPGAGLAFLAGNVPILRK
                     WVVRGIQAAIPASEAELTAPLTEWHTYSLHWETARVTCEVDGVTVLDAPVAPRGPLGF
                     VAWIDNQFAVLSERTGIRFGVVPTTAPAWLEISDLEIRSPAVT"
     gene            3030..3102
                     /locus_tag="VI520_08115"
     tRNA            3030..3102
                     /locus_tag="VI520_08115"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3063..3065,aa:Phe,seq:gaa)
     gene            complement(3138..4199)
                     /locus_tag="VI520_08120"
     CDS             complement(3138..4199)
                     /locus_tag="VI520_08120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLF37884.1"
                     /translation="MLGDCLRVMERLPQGSIDLIFADPPYNLQLSQELWRPNQTRVHG
                     VEDAWDRFESFKQYDEFTRAWLTACRRVLKADGSLWVIGTYHNIYRVGAILQDLGFWI
                     LNDVLWIKTNPMPNFRGVRFTNAHETLIWAAKSSSSKYTFNHHAMKRFNDGKQMRSDW
                     VVPLCTGKERIKLNGKKAHSTQKPEALLYRVLLASTEPGDIVLDPFFGTGTTGAVAKR
                     LGRQWIGIEKEPGYVEIARRRIEAVEPEPAEVQALEVRSARREPRVPFASLLEAAFIR
                     PGQKLYFKKDRSRSAWVKPDGRLRLNGIEGSIHQLGSHLAGGGPCNGWEHWFYESQSG
                     ALEPIDRLRERLREGGPQK"
     gene            complement(4395..4706)
                     /locus_tag="VI520_08125"
     CDS             complement(4395..4706)
                     /locus_tag="VI520_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37885.1"
                     /translation="MGVFYVLVTMAIQGEIRIGRGELGETRVWLLREGLERGLGFSGT
                     RVVSGSEATGAACVETQVRFLILGGAGGSAPVSYCECLEKVGETWTSIGECSAQSSVA
                     D"
     gene            4997..5068
                     /locus_tag="VI520_08130"
     tRNA            4997..5068
                     /locus_tag="VI520_08130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5029..5031,aa:Gly,seq:gcc)
BASE COUNT          905 a         1575 c         1561 g         1043 t
ORIGIN      
        1 cagggattaa cttcgtgatt cgcttcggga ccaactcctt gctctttgtg atggacggtg
       61 tcagctagcc gagcaggcgg ccttctcgct ctggacgaat ccagcggcgg gaccaaaggg
      121 tgagcaggtc aatcatgggc cgcaattcct cccccttatt gctcagttcg tattcgactg
      181 ttggggggaa ggtcgggatc accgtgcgct taagcaagcc ggctttttcc agctctcgca
      241 accggcgggt caacgtcgta gggctgatat catgggtcac ttcctgaaga tcacagaatc
      301 gtttgtttga gcgcaggaag tacagcagct ccaaggtcca tttccttccg agcagtcgag
      361 cgatgcttga aataggacaa atgtggctgg aggccttcga aggcatggcg ccaatactac
      421 actttctgta gcattgtggc gagggagact gccggccggc ggatcggaac ccgcgggccg
      481 cttgagcggg ctcacccaag ggggctgcgc cgttaactgc gctcttacgc gggtcatggt
      541 ataagtgaac gaattcgagc cggagtaagg ccggccggag cgactgaacc atgcctatct
      601 acacctaccg ctgcggcaac tgcggagtgc agtttgagcg caagcagggc tttgaggatc
      661 cgctgctccg caagtgtccc gagtgcggtg agacaaccct gcacaaactg tttaccccgg
      721 tcggcatcca cttcaagggc ccaggtttct attcgaccga ccacggctcg ggctctcgat
      781 cgaatggctc cgccaagtcg gagccgaaga aggaagattc tggctctgcc aagccttccg
      841 agccgagccc gaagccagat aagaagccaa ctagcgaaga ctgagtcgcc ccggaccagg
      901 tgtcatcgta gcttaaatga agagacgccc tgccgggcgt ctctttgttt cctaaatctg
      961 gaagccgccg gaggccttca ccactttcat gctgaagttg gtgacatact cgcccaggtg
     1021 gccgtgaagc tgctgccagt cgtcactggt gagcgcctgc tgcatcaagg cgagggtgtc
     1081 ggcgattccg ccggtcagaa tctggggctg gtcgccgtac tgagtgaacc aggcctcggt
     1141 cggctgaatt ccgatcttct gcattcctgg cacaaactct cgaaccacga actcaaagta
     1201 ttcctgctct ctgccaggca tgatgtccca ggtcatgatg agtttgacgg gcattgggga
     1261 atgggcggat caggccgatc gctgcagcac gaccacttgg gtttgatccg agagcccgct
     1321 tttcgtgacg gttagccgct cttgcggctc ttgcttgccg atgcccatct cggagaggaa
     1381 gccggccacc tggcccagct tgatcttttc accgccggtc ttgaactgca tcggcacgac
     1441 cagcgagggc tcgatcagac tgatcacctc ggccgcctcg gccggtgtga gcccctcgcc
     1501 gccaccgacc ggaaccaacg caatgtctac cgggccgagg tcctcgatct gggcctgggt
     1561 cggcacatag gccaggtcgc ccaggtgggc gaccgtgagg ccttcgaatt caaagacata
     1621 cagcgtgttg gggaccttac cgtttttccg ttggcccttg gcggccatcg agatcccggt
     1681 gatgaacacg cccccgactt cgtattcgcc ggggccggtt acccgcaggc gctcgccttt
     1741 gacgccgccg aaattgttgt ggccggggga gtcgtggctg atggtcacga cgtctgcggt
     1801 gagcttaagg gcgggcaggc cgagcgccgt atcgtaggga tcggttacca cggtcgccag
     1861 gccgcgttct ttcattcgga aacaagagag gccgtaccag acgatttcca ttagcggctg
     1921 ccctgcggca cgaccgggcg attatacctg aagcgactca aaccggccgc ggacccgggt
     1981 gctgagagtg tagtccgccc ctataatgcg gtccatggga gcgcttcgct atggtcttaa
     2041 gccgcacttg ctgggcggtg ggcaggtttt cccgatcgat gcccgtagtt ttcgactgga
     2101 gattcctaca ttgccgcgcg gctacgccga cgcccaggtc gacgactgca agggccgccg
     2161 acgctctcag ttcgcctggg ccccgccggc cagtctgacc ctgcgggcgc gggcctctca
     2221 ccccgcccct ccgggaacga tgggtttcgg gttttggaac gatccctttg ggttgtcgct
     2281 gggcggcgcg ggtggagtgc agaagttgcc agccgcgccg caggcggttt ggttcttcta
     2341 cggcgcaccg cccaacgata tgaactttgt cagtggtgga cgcggcgatg gttggaaggc
     2401 cgcgagcctc cgctcgcccg cagtcccggc cgcgctgctg gttccgggcg ccgggttggc
     2461 cttcttggcc gggaatgtcc caatattaag gaagtgggtc gttcggggga tccaggcggc
     2521 catcccagcg tcggaggcgg agttgaccgc gccgctcact gagtggcaca cctattcatt
     2581 gcattgggag accgcccggg tgacctgcga ggtggatggg gtgacggtac tcgatgcccc
     2641 ggtggccccg cgcgggccgt taggatttgt ggcctggatc gacaatcagt ttgcggtgct
     2701 ctcggagcga actggaatta gattcggggt cgtgccgacg acggctccgg cctggttgga
     2761 aatcagtgat ctggagatcc gtagtcccgc ggtaacctga gggcgcgcct cccacccgag
     2821 tactttgcct gggcatgcct ctgtaagcag agtgtaacgt cgccgtctgg ccggcgcgca
     2881 taccatggct gtgcagaccg gtgtgacttg cctgggtgac cacagctagg gcaaaaacgt
     2941 ctggtgatga ccccctccag acccccagaa gccgcgccgg ttctgctttt cttgacaccc
     3001 ctctgtgcgc tagttacaat ccggccggtg ccgagatagc tcagttggta gagcacgcga
     3061 ctgaaaatcg cggtgtcgcc agttcgattc tggctctcgg cacctcggac tgattaacac
     3121 accccgccgg ggtgtcttca tttttggggc ccaccttcgc gtaaccgctc gcgcagccgg
     3181 tcaatcggtt cgagcgcgcc actttgtgat tcgtaaaacc agtgctccca tccgttgcag
     3241 gggccgccgc cggccaggtg gctgccgagc tgatgaatcg atccctcgat cccgttcagt
     3301 cgaagtcggc catcgggctt gacccaggcc gatcggctcc gatccttttt gaagtacagc
     3361 ttctgccctg gtcgaatgaa ggccgcctcg agcagcgagg cgaacggcac ccgcggctcg
     3421 cggcgagctg accggacctc gagggcctgc acctcggcag gctctggctc aaccgcctca
     3481 atccgccggc gagcgatctc cacgtaaccg ggctccttct caatcccgat ccactgccgg
     3541 cccaggcgct tggcgaccgc gcccgtggtc ccggtcccga agaatggatc cagtacgata
     3601 tctccgggtt cggtcgaggc aagcagcacc cgatagagca gcgcttctgg cttctgggtc
     3661 gagtgagctt tcttcccgtt gagcttgatc cgctccttgc cggtgcacag cggcaccacc
     3721 caatcgctgc gcatctgctt gccatcattg aagcgcttca tcgcgtggtg gttgaaggtg
     3781 tacttggaac tgctggactt ggcggcccag atcagcgtct cgtgggcatt ggtgaaacgc
     3841 accccgcgaa aattgggcat cggattggtc ttgatccaga gcacgtcatt caggatccag
     3901 aatccgaggt cctgcaagat cgccccgact cggtaaatgt tgtggtaggt cccgatgacc
     3961 cacagggagc cgtcggcctt gagtaccctg cggcaggcgg taagccaggc ccgggtgaac
     4021 tcgtcgtact gcttaaagct ctcgaatcgg tcccaggcgt cctcgactcc atgcacccga
     4081 gtctgattgg ggcgccaaag ttcctggctg agctgcaggt tatagggtgg atcggcgaag
     4141 atcaggtcga tggaaccttg cggcaggcgt tccatgactc gcaggcagtc gccgagcacc
     4201 acctgattta gtggcagcgt tctcgtaggt atctcgccct tccctttatg gccgaggcca
     4261 tgtccaggtg agtgcagctt cagtgggctg cacccgcccg gcggcgcgga gtctcgatct
     4321 aataacgcac ccgacgatct agctcatggg tcgatctttg atggcctcca atgttattcg
     4381 gctatgcgca gccttcagtc agcaaccgat gactgcgccg aacactcacc gatcgaggtc
     4441 caggtctctc caaccttctc caggcactcg cagtaactga ccggtgccga ccctcctgcc
     4501 ccgcccagta tcagaaagcg aacctgggtc tcgacacatg ccgctcccgt cgcctcgctc
     4561 ccgctgacga cccgtgtgcc ggagaaaccc agtcctcgtt ccaggccctc gcgaagcagc
     4621 caaacccgag tctccccaag ctccccgcgg ccgatccgaa tttcgccttg gatggccatc
     4681 gtcaccagca cgtagaacac gcccaccgcc agcagcccga tcccgatgct gatcaggcat
     4741 ccgatagcgg ggggaaaggg aacgttgctc tccggctcgc gtcgatgctg cgcggttcca
     4801 ggggtgggca gccaagacct ccatcctcga ttgggatcag gctcacgcga agtctactca
     4861 gttagctcgc cggggcaact tcgccctgga actagatgag cgtggcccgt gtctgcgccg
     4921 accctggtag gccaaaccgt gtgccgcgcc ggccctgctt gactacctac gggcttaacg
     4981 atatcctcgt gcttacgcgg gtgtagttca gtggtagaac gtctccttgc caaggagaag
     5041 gtcgtgggtt cgaatcccat cgcccgctct gagtaggcgg ccta
//
LOCUS       DATDPQ010000363         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_458777, whole genome shotgun sequence.
ACCESSION   DATDPQ010000363 DATDPQ010000000
VERSION     DATDPQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458777"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VI520_08135"
     CDS             complement(<1..384)
                     /locus_tag="VI520_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLF37886.1"
                     /translation="MKRTAVLTGSASGIGKATRELLLARGYRVLGVDLHEAEVIADQS
                     KPEGREAMLYAVRWMTGGVVDAVIANAGLARPEPITGSVNYFGAIAALEGLQPMLVKG
                     QQPRAVLVASLANTFPHDEALVEAYL"
     gene            complement(564..1001)
                     /locus_tag="VI520_08140"
     CDS             complement(564..1001)
                     /locus_tag="VI520_08140"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HLF37887.1"
                     /translation="MATRTKKGKNKPTKPAAGPSRQPAPRPPRKNPDVDEWFDRLDHP
                     LKILMLQVRRVILASDRRVNESIKWSTPTFSYNGDIASFIPQAKRFVSLLFHRGAEIP
                     GKHPRLEGDSRLARTMRFASPAELKKHTADLENAIRAWCEFKS"
     gene            complement(1043..1426)
                     /locus_tag="VI520_08145"
     CDS             complement(1043..1426)
                     /locus_tag="VI520_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLF37888.1"
                     /translation="MKLNRIHQIAVHARDLDEAITFYRDTLGASYLGKYDPPGLAFLD
                     FSGVRVLLEKTGAKSTMYFRVDDIDSACAELRSRGIEFEAGPHLIHRDDTGVFGKPGE
                     EEWMAFFSDPSGNNLALATRRSAAG"
     gene            complement(1427..1960)
                     /locus_tag="VI520_08150"
     CDS             complement(1427..1960)
                     /locus_tag="VI520_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37889.1"
                     /translation="MAVKKIQINRAPVMTLWATVVAERLGYDPEAALSLGKAVAGLNA
                     QSKGRRLGIYQSTSEQETPKGKPKPPEPTIVTLLGRPVPTLRTKHGVRAAIRGEAIEP
                     RPVQEYLVKKFGADLAEVRKVMEALARSLEPEALEAQAFGLYEKFRPQIPEGKRGWGA
                     AGELDLGQLHRLAKRKA"
     gene            complement(1999..3486)
                     /locus_tag="VI520_08155"
     CDS             complement(1999..3486)
                     /locus_tag="VI520_08155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLF37890.1"
                     /translation="MLAEPPARSRAVTIGTQSTILILFLSLSALGLRFVYLEADFPYQ
                     INWSGDLYTDEGWYSNNAIAHELTGGWIVEGDFNPIVSLPVFPLLQAGAFNLLGFGLT
                     TARITEVVFSILVCLLSYRLVTRMADGLSGLATLFLLSTNFTVFAFSRLAILELPMTA
                     LTLVSLLLAVSTRGPRALNLALASLAFCLATLTKTTALFGLPSFLYLIWTAQPSSRKG
                     MLAASAALLLVGLVLGVYYAVADAGDPESVALFAATEFTPRLEGTLPSIGWALGRTVW
                     NGLVLDRLAYPLAILSLPLYLVLSRRARTDRLVIACTIWIVSSFAALAVRGYLPPRYY
                     LPLSVPLACLLGVIAVRSFQAFRPSPRSYAPLVLLAGIGAVNLIGIISHLASPQFSFI
                     EMAQDVEAQIEARGGDPLLIGNIANSVSLATGVPSINSELGTRDLAWKLEKYRPGYFL
                     ALGEEKPTVRELSEAYELQPLASYDVFGNYYDGKQVQLYKLILNP"
     gene            complement(3518..>3735)
                     /locus_tag="VI520_08160"
     CDS             complement(3518..>3735)
                     /locus_tag="VI520_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37891.1"
                     /translation="TEEFEALRKGLGYCWSVAVAAFPEQGRPRMERWIGSPDPDVQWV
                     MKQNLRKTRLTRLDPKWVARAQQRLKL"
BASE COUNT          706 a         1142 c         1188 g          699 t
ORIGIN      
        1 gaggtaggcc tcgaccagcg cttcatcgtg agggaaggta ttggcgagcg atgcaaccaa
       61 cacggctcgc ggctgctgtc ctttgaccag cattggctgc aagccttcga gcgcggcgat
      121 ggcgccgaag tagttcaccg agccggtgat cggttccggc cgcgccagcc cggcgttggc
      181 gatgaccgcg tcgacgacgc ctccggtcat ccatcgcacc gcatagagca tggcctcccg
      241 gccctccggc ttggactggt cggcgatgac ctcggcctca tgcaggtcga ccccgagcac
      301 ccggtaaccc cgtgccaaca gaagctcgcg agtcgccttt ccgattcccg aggccgagcc
      361 ggtgagtaca gctgttcgtt tcatggagcg cctccttctg cggtgagcgg ggtggctctc
      421 gaccctccct atcctcacac gcggccactc cggattgcag ggacgctcat cactcgccac
      481 gcccgccatt cgtcctctct tggcgggctt cggcgtacgc tggtcgaacc tcggtgagag
      541 gcgcctgacg ggttgccgac ccgctagctc ttgaattcac accaggcgcg gatcgcgttt
      601 tcgaggtcgg cggtgtgctt cttgagctct gcggggctgg cgaagcgcat cgtccgagcg
      661 agccgcgagt cgccctccag gcgagggtgt ttccccggga tctcggctcc ccggtggaag
      721 agcaggctca cgaagcgttt cgcctggggg atgaagctgg cgatgtcgcc gttgtacgag
      781 aaggtcggag tactccactt gatagactcg ttcacccggc gatcggaagc caggatcacc
      841 cgccgaacct ggagcatgag gatcttgagc gggtggtcga ggcggtcgaa ccactcatcg
      901 acatctggat tcttgcgcgg cggccgcggg gcgggttggc ggctgggacc cgcagctggc
      961 ttcgtgggtt tgttctttcc cttcttggta cgtgtggcca attcgacctc cttactttgt
     1021 aagctctccc cttagcgact cgctaaccgg ccgcggatcg ccgggtcgcg agcgcgagat
     1081 tgttgccgga gggatctgaa aagaacgcca tccactcttc ttctccgggc ttaccgaaca
     1141 cgccagtgtc atctcggtga atcaagtggg ggccagcctc gaattcaatt cccctggacc
     1201 taagttcagc gcaagcggaa tcgatatcat cgacccggaa atacatcgtg gacttggcac
     1261 cggtcttctc gagcaacacc cgcacgcccg aaaagtccag aaatgcgagg cccgggggat
     1321 catacttccc caggtaactg gcaccgagtg tgtctcggta gaaagtgatc gcttcatcga
     1381 gatctcgggc gtgcacggca atctgatgga tccgattgag tttcattcag gccttccgct
     1441 tggcgagacg atgcagctgc cccaggtcga gttcgccggc cgccccccag cctcgtttgc
     1501 cttccgggat ctggggccgg aacttctcgt acaacccaaa cgcctgggcc tcaagggctt
     1561 ccggctccaa cgaccgcgcc agggcctcca tgaccttacg gacctcggcc agatccgccc
     1621 cgaacttctt gaccaggtac tcctggaccg gccgaggctc gatggcctcg ccgcggatcg
     1681 ccgcccggac cccgtgcttc gttcgcaggg taggaaccgg tcggcccagc agggtgacga
     1741 tggtcggctc tggtggtttg ggcttgccct tgggcgtttc ttgttcactg gtcgactggt
     1801 aaatcccaag tcgccggccc ttcgattggg cattgagccc tgcaaccgcc tttcccaggc
     1861 tcaaggcggc ctcggggtcg tagccgaggc gctcggcaac gacggtcgcc cataaggtca
     1921 tgaccggtgc ccgattgatc tgaatcttct tgacagccat ctcaaccaat cctccccgtt
     1981 gcttgccgga atgccgacct atgggttcaa gatcagtttg tagagctgaa cctgtttgcc
     2041 gtcgtaataa ttgccgaaca catcatagga ggcgagaggc tgcagttcgt acgcttcgct
     2101 cagctcacgt acggttggtt tctcttcgcc caacgcgagg aagtagcccg gacggtactt
     2161 ctcgagtttc caggccaagt cgcgggttcc cagctcggaa ttgattgaag ggaccccggt
     2221 agccagcgag acggagttgg caatatttcc gatcagtagc ggatcacctc cgcgggcctc
     2281 gatttgcgct tcgacgtcct gggccatttc gatgaagctg aactggggcg aagccaggtg
     2341 gcttatgatg ccgatgaggt ttaccgcgcc gatgccggcc aatagcacca acggggcgta
     2401 ggaacggggc gaaggccgga aggcctgaaa cgagcggacc gcaatcactc caagcaggca
     2461 ggccagcggg acgctcagcg gtaagtagta gcggggtgga aggtagccgc ggacggcgag
     2521 tgcggcaaac gagctcacga tccatatcgt gcaagcgatg accagccggt ctgtccgggc
     2581 tcgccgggag aggaccagat acaacggcag ggacagtatc gccaagggat aggcgagccg
     2641 gtcgagcacc aacccgttcc agaccgtccg gccgagcgcc caacctatgg aaggcagggt
     2701 cccctccagc cggggggtga actccgtggc ggcaaagagt gcgaccgatt caggatcccc
     2761 tgcatcggcg acggcatagt aaacgcccag aaccagcccg accagcaata gagcggcaga
     2821 ggcggccagc attccctttc ggctggaagg ctgcgcggtc caaatcaggt agaggaagct
     2881 gggcagcccg aacagcgcag tcgtcttcgt tagggtagcc aggcagaacg cgagtgaggc
     2941 gagagccagg ttcagcgccc tgggtccgcg ggtggagacc gccaggagca ggctcaccaa
     3001 cgtgagggcg gtcatcggca gctcgagaat ggccaaccgg ctgaaggcga agacggtgaa
     3061 gttggtggag agcaggaaaa gggtcgccag tccggaaaga ccgtcggcca tccgggtcac
     3121 cagccggtag ctcagcaagc acaccagaat cgagaagacg acctccgtga tcctggccgt
     3181 ggtcagcccg aatccaagca ggttgaaggc gcccgcctgg agcagtggga agaccgggag
     3241 gctgacgatc ggattgaagt cgccctcgac gatccagccg ccggtgagct cgtgggctat
     3301 ggcattgttg gaataccaac cttcgtcagt gtacaggtcg cccgaccagt tgatctggta
     3361 tgggaaatcg gcttccaaat agacgaagcg cagcccgagg gccgagaggc tcaagaatag
     3421 gatcaagatt gtcgactgag tgccgatggt tacagctcgc gagcgagccg gtggttccgc
     3481 cagcaagtta gctccgggtt gacagtcgtg gattcggtca gagcttgagc cgctgttgtg
     3541 cccttgcaac ccacttcggg tcaagtcggg ttaggcgggt ctttcggaga ttctgcttca
     3601 tcacccactg cacgtcagga tccgggctgc caatccaccg ctccattcgc ggccttccct
     3661 gctcggggaa ggcggcgacc gcgacgctcc agcagtagcc caggcccttg cgaagcgcct
     3721 cgaattcttc cgtct
//
LOCUS       DATDPQ010000364         7072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_206589, whole genome shotgun sequence.
ACCESSION   DATDPQ010000364 DATDPQ010000000
VERSION     DATDPQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206589"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VI520_08165"
     CDS             <1..1548
                     /locus_tag="VI520_08165"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37892.1"
                     /translation="LAYHYREAKDDEKALKYYTLAGNMARRISAYSEAITQYGHALDI
                     ANQLGASPGQLSFLYTRRGRIFELAGRYDEALANYAELQEQGRKLNELALEMSSLNAQ
                     ATIHAIPSGKWDPARAEELAKAALVLAQQLSDPRGEAKALWNLLLVEVYSERDPLAGI
                     NYGERSLAIARQHNLIEEQAFAHHDLSRAYGQAGRFEDGHRALEESRALWEQLGNTEM
                     LADNLTSSAYYYSLLGDFGQAIALAERSLELSRKVGNAWGQAYSLMSLGLARFERGEL
                     SEAIRTFQESIARGKEAAFAGAGTFVPTFLALTYARLGAEGRALELLESALAITDQQD
                     QTRGFALALKAVVLADQGNLAEAQSLARAPVENNVTNPEFVGFGGLLRADVLLAIQDF
                     QATLELVDEKLDQLAALNARYFLPQFRLRRARALRGLGKTTEAQDVLEQARREAEDMG
                     LQMTLLSILLELAELAGELGDEPKAQSSRQAAGEVVRFITDHVDEEELRKSFLALPHI
                     HSVTALV"
     gene            1716..5234
                     /locus_tag="VI520_08170"
     CDS             1716..5234
                     /locus_tag="VI520_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF37893.1"
                     /translation="MAATAPFSTQEKLLQYIPKELLAKLEAARAGHSMEGERRVVTIL
                     FCDVKGSTAAAESMDPEDWAEVMNGAFEHLIAPIYRLEGTLARLMGDAILAFFGAPIA
                     HEDDPERAVLAGLQIVNGIAPYRDQVHAQWGLDFNVRVGINTGLVVVGEVGSDLRLEY
                     TAMGDAVNLAARMEQTATPGTVQVAEETYQRAAPLFEWESLGPVSIKGKAEPVPAYRA
                     LTRKERPRRLRGIEGLYSPLVGRLTEMSVLHESLDGLGRGHGGIVALIGEAGLGKSRM
                     IDELHSSWESGTPGGYWGESRGISYETTRPYAQFQQQLLQLCGVVQPDDLGEVRRKLA
                     SVVAEFPQDEQARAGLALQAVLGAEDPQDGGTLEGEALRRELYRTCAAVWRRQAARQP
                     TVLVFDDLHWSDPASTELLIDLFRLTDAGPILFLCAFRPDRQAPAWQVKQAGETVYPH
                     RYREVVLQPLSSGSSRELVDHLLSVSDLPPKLHSLIEQKAEGNPFFVEEVIRTLIDRG
                     AVLRDANGTRWKPGTDIDEIAIPDNLQGLLVARIDRLADETRRTLQLASVVGRTFYYR
                     VLQAISEAADELDGRLSDLQRSELIREAARDPELEYAFRHALTQEAAYGSILRKERRL
                     YHLRVGDALERLFPDRLSDLAPLLAHHFFEAADERALKYAVLAGDQAFRMYAIESAIQ
                     HYRRALDLELRSPTEDTQLLRRLYTQLGRSYELIDRFDAALELYQQMRATGHERSDGE
                     MELSSLMESAKIFSTPNQLFNADRGRAASDEAVQLARQIDNHQAEARILWNRMNSENY
                     LGEMSSALEFGKRSLAIAEEFNLKDQLAFTLNDLTLSLLTLGQVDRAQEVNQRARALW
                     KELGNLPMLADNYSTWSFGALWLGEFEQAVVEAEEAYRITKSIDNTWGQATSRLWIGL
                     AYLEQGRFEAALEVQRDGLRLALEAEVPPAVMFMGAFLGMSYGVIGSLPDGFAALKEA
                     LQVDPTQFLSFACMPRSVLARLHLWNGDIPQARLAMQSSYEGFKPVGSMHAADQVLIT
                     DAEIELAAGDSSKAIERIDQMLSLARAAGRRLYLPEALLLRGRALLAQSALTQAGENL
                     QEAHQWADQLGSRRVLWQILLEQSRLAAKSGDPDRAEQLRAAAGQAVIQLTASISNPD
                     LRRSFAALPDAREALAGQVGAGPTAG"
     gene            5289..5804
                     /locus_tag="VI520_08175"
     CDS             5289..5804
                     /locus_tag="VI520_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLF37894.1"
                     /translation="MDTVSYLREDMGWAHGLLELVMQDATQEQLDWAPPGKANPLGAT
                     YAHALTSEDMIVQRWLQGAEPLFESAWRGRTGISEPQFGSDFEWARRVRVELPVAREY
                     AHAVYAASDQYLGSLEPGALDLVLDLSKQGFGPKAVAWVLSALVVSHMNNMIGESAVL
                     KGIQGAKGYPW"
     gene            5843..>7072
                     /locus_tag="VI520_08180"
     CDS             5843..>7072
                     /locus_tag="VI520_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF37895.1"
                     /translation="MAKPVILTVDDDPQVLNSVERDLRQHFRGEYRIVKADSGSQALQ
                     AVQELKRRNDPLALFLVDQRMPEMTGTEFLTQAIQLYPEARKVLLTAYADTEAAIQSI
                     NTLGLDHYLMKPWEPPEQNLFPVLDDLLSDWRAMTTLPYEGIRVAGTLWSPSSHAVKD
                     FLARNQIPYQWLDVEADAQARELVKSTDGGEPRLPVVFFPDGSFLVEPDNPALAAKVG
                     LQTEASRQFYDLVIVGGGPAGLGAAVYAASEGLRTAMVERHSPGGQAGTSSLIENYLG
                     FPKGLTGADLARRASTQAKRFGAEIITAVEAAKVRVDGPYRTVTCTNGSELNCHALLI
                     ATGVTLRRLEIPGADELTGRGVYYGASLTEAAYYKDQHVVVVGGANSAGQGAMFFSLY
                     ASKVTMLLRASSLSKSMS"
BASE COUNT         1345 a         2206 c         2240 g         1281 t
ORIGIN      
        1 ttggcctacc actaccggga agccaaggac gacgagaagg ccctcaagta ctacaccctg
       61 gccggcaaca tggccaggcg tatttccgcc tactcggagg ccatcaccca gtacggccat
      121 gccctggaca tcgccaatca actgggcgcc agtccgggac agctcagctt cctgtatacc
      181 cgccgcggac gaatcttcga gctggccggc cgctacgacg aggccctagc caactacgcc
      241 gaactccagg agcagggtcg gaagctcaac gagcttgcgc tggagatgtc atccctcaat
      301 gcgcaggcga cgatccacgc tatacccagc ggcaaatggg atccggcgcg ggccgaggaa
      361 cttgccaagg ctgccctggt gctcgcccag cagctgagcg acccgcgggg cgaggcgaag
      421 gccttgtgga atctgctcct ggtcgaagtc tattccgagc gcgatccgct cgccggtatc
      481 aactatggcg agcgctcgct cgccattgct cgccagcata acttgatcga agagcaggct
      541 ttcgcacatc atgacctttc gcgggcctac ggtcaggcgg gtcgatttga agacggccat
      601 cgagccctag aggaatcccg cgcgctctgg gagcaacttg ggaacactga gatgcttgcc
      661 gataacctga cctcctccgc ctattactac tcgctgctcg gagatttcgg ccaagcgatt
      721 gccctcgcgg aacgatccct ggagctcagc cgcaaggtcg ggaacgcctg ggggcaggcc
      781 tacagtctga tgagcctcgg gctggcgcgg ttcgaacgag gtgagctatc ggaggcaatc
      841 cgcacgtttc aagagagtat cgccaggggg aaagaggcgg cattcgccgg cgcagggacg
      901 ttcgttccaa ccttcctggc cctgacttat gcccgcctcg gtgcggaagg ccgcgccctg
      961 gagctgctcg agagcgcgct ggcaatcaca gaccagcagg accagacccg gggctttgca
     1021 ctcgcattga aggcggttgt gctggcggat cagggcaatc ttgctgaagc tcaaagcctg
     1081 gctcgcgcgc cagtggagaa caatgtcacg aatcccgagt tcgttggctt cggcggcctt
     1141 ctgcgtgccg acgtattgct ggcgatccag gattttcaag ctacgctcga gctggtagac
     1201 gaaaaactcg atcagcttgc ggccttgaac gcacgctact ttctgcccca gttcaggttg
     1261 cgcagggccc gggcgctgcg cgggctaggc aagactaccg aggcccagga cgtgctggag
     1321 caggcccgcc gcgaggccga ggacatgggc ctgcaaatga ctttgctgtc gatcctgttg
     1381 gagctcgcgg agctggccgg tgaacttggc gacgagccga aagcccaaag cagccgacag
     1441 gctgccgggg aggttgtccg gttcatcacc gatcatgttg acgaggaaga attgcggaag
     1501 tctttcctcg ccctccccca cattcactct gtcactgcct tggtttagtc acccaccccg
     1561 acagccaatc catgaattgc ccagagtgct cggccgaaaa cccggaggcc gcccggtttt
     1621 gcagccagtg tgggggctca ctgacccggg cctgtcccaa ttgtgggaca aagaacaacc
     1681 tcgccgccaa tttctgcagt aactgcgggg agcgattggc agctaccgcc ccgttctcca
     1741 cccaagaaaa gctgctccaa tacattccca aggaactgct cgccaagctc gaggctgccc
     1801 gagcggggca cagtatggaa ggcgagcgcc gggtagtgac gatcctcttt tgcgacgtca
     1861 agggctcaac cgcggcggcc gaatccatgg atccggagga ttgggccgag gtcatgaacg
     1921 gagccttcga gcacttgatc gccccaatct accggctcga aggcaccctg gcccgtttga
     1981 tgggcgacgc gatcctggcc ttcttcggcg caccgatcgc ccacgaggac gacccagaac
     2041 gagcggtgct cgccgggctg cagatcgtga acgggatagc gccctatcga gaccaggtgc
     2101 acgcccagtg ggggctcgat ttcaatgtgc gggtggggat caacaccggc ttggtcgtgg
     2161 tgggcgaagt cggctccgac ctgcgcctgg aatacaccgc gatgggggat gcggtcaacc
     2221 tggccgcccg catggagcaa accgccactc ctggcacggt ccaggtagct gaggagacct
     2281 accagcgcgc cgctccactc ttcgaatggg aatcgctcgg tccggtgagc atcaaaggca
     2341 aggccgagcc ggtgcctgcc tatcgggcgc tcactcgcaa ggagcgcccc cggcggctgc
     2401 gagggatcga aggcctttac tccccgctcg tcggacgtct gaccgagatg agcgtgctgc
     2461 acgagtcact ggacggcctg ggccgtggtc atggggggat cgttgccttg atcggggagg
     2521 ccggcctcgg caagagccgc atgatagacg agctgcacag cagttgggaa tctggaaccc
     2581 ctggtggcta ctggggcgag agccggggga tttcatacga aacgactcgc ccctatgccc
     2641 aattccagca gcaactcctc cagctgtgcg gcgtcgtcca gccggacgac ctcggggagg
     2701 ttcgtcgaaa gctagcttcc gtggtcgcag agtttccgca ggatgagcaa gctcgggccg
     2761 gcctggcgct gcaggcggtc ttgggtgcag aagatcctca ggacggaggc acgctggaag
     2821 gagaggcgct ccggcgggaa ctctaccgaa cttgcgcggc ggtctggcgc cggcaggcag
     2881 cccgacagcc caccgtgttg gtgttcgacg atctgcattg gtccgacccg gcttccaccg
     2941 agctgctcat cgatctcttc cgtctgacgg atgccgggcc gatcttgttc ctgtgtgcct
     3001 ttcgtccgga tcgccaggca cccgcctggc aggtcaaaca ggctggcgag acagtttacc
     3061 cgcaccgcta tcgggaggtc gtgctgcagc cactctcttc cggaagcagc cgcgagctgg
     3121 tcgaccacct gttgtcagtt tcggatctgc ctccaaagct ccactcgttg atcgagcaga
     3181 aggcggaggg aaatcccttc ttcgtggagg aagtgatccg gactctgatc gatcgcggcg
     3241 cggtactgcg cgatgccaac ggtacccgct ggaagcccgg gacggacatt gatgagatcg
     3301 ccatcccaga caacttgcag ggtctgctcg tagcgcggat cgatcggctg gcggacgaaa
     3361 cccgccgcac cttgcagctc gcttcggtgg tcgggcggac tttttactac cgcgtgctgc
     3421 aggccatctc ggaggcggcc gatgaactcg acggccgctt gagcgatctg cagcggtccg
     3481 agctcatccg ggaggccgcc agagatcccg agctggagta tgccttccgt cacgcgctga
     3541 ctcaagaagc agcctacgga tcgatcctgc gcaaggaacg gcggttgtac catctccgag
     3601 tcggggatgc cctcgagcgg ctcttccccg atcggctttc agatctggcg ccgctgctgg
     3661 cgcaccactt cttcgaagcc gcagatgagc gggcgctcaa gtatgcagtg ctagccgggg
     3721 atcaggcctt ccggatgtac gccatcgagt cggcgatcca gcattatcgg agggcacttg
     3781 acctagaact gcgctccccg acggaggata ctcaactcct gcggcgccta tacacccagc
     3841 tgggaagatc gtacgagctc attgatcgat tcgatgcggc gttggagctc taccagcaaa
     3901 tgcgtgcgac tggacacgag cgctccgacg gagaaatgga gctcagctct ctgatggaga
     3961 gtgccaagat attctcgact ccaaatcaac tattcaacgc cgatcgaggc cgggccgcct
     4021 ccgatgaggc cgtccagctg gctcggcaga tcgacaacca ccaggcggag gcccggatcc
     4081 tgtggaaccg gatgaacagt gagaactact tgggagagat gtcaagcgcc ctcgagttcg
     4141 ggaagcgttc cttggccatc gccgaagaat tcaatctgaa ggaccagctg gccttcacac
     4201 tgaatgacct gactctctcc ctgctcacgc tgggccaggt agaccgagcc caggaagtca
     4261 accagcgagc acgggcgctc tggaaggagc ttgggaatct tcccatgctg gcggataact
     4321 actccacgtg gagctttggg gccctttggc tgggggaatt cgagcaggcg gttgttgagg
     4381 ccgaagaggc ctaccggatc acaaagtcca tcgataacac ctggggccag gccaccagcc
     4441 ggctctggat cggattggcc tacctggaac aggggcgatt cgaggccgct ctagaagttc
     4501 agcgtgacgg cttgcggctt gcgctcgagg cggaggtgcc tccggccgtg atgttcatgg
     4561 gagccttcct ggggatgagc tacggtgtca tcggttcgct cccggatggt ttcgccgccc
     4621 taaaagaagc ccttcaagtg gatcctactc agtttctcag cttcgcctgt atgcctcgaa
     4681 gcgtactcgc ccgactgcac ctctggaacg gcgatatccc ccaagcgcgg ctggccatgc
     4741 agtcaagcta cgagggtttc aagccggtcg ggagtatgca cgcggccgac caggtgctga
     4801 tcacagacgc ggagatcgaa cttgctgccg gtgacagttc gaaagcgatt gagaggattg
     4861 accagatgct cagcctggct cgggccgccg gacgccgact gtacctgccc gaggctctgc
     4921 tgttgcgggg ccgtgcgctg ttggcgcaat ctgctctgac ccaggcgggt gagaacttgc
     4981 aagaggcaca ccaatgggca gatcagctcg gctcgcggcg cgtgctgtgg cagatcctac
     5041 tggagcagag ccgtctggct gctaagtccg gagacccgga tcgggcggag caactgcgcg
     5101 ccgctgccgg gcaagccgtg atccaattga ctgcgtcgat cagcaacccg gatctacggc
     5161 ggtcatttgc ggccctcccg gatgcccgcg aggcacttgc cggacaggtg ggcgccgggc
     5221 caactgcggg ctgaccctcc tcaagtatca ttgcgctgtg ggcacgccgt tactgcggag
     5281 gaacgtccat ggataccgtt tcttatctgc gtgaggacat gggttgggcc catgggctgc
     5341 tggagctggt tatgcaggac gccactcagg agcagctcga ctgggctccg ccgggcaagg
     5401 ccaacccgct gggcgccacc tatgctcacg ccctcaccag cgaggacatg atcgtccagc
     5461 gttggctgca gggcgcggag ccgctgttcg agtcggcatg gagaggcaga accggaatca
     5521 gcgagccgca gttcggctcc gatttcgagt gggcccggcg tgtgcgggtg gagctgcccg
     5581 tcgcccggga gtacgctcac gcagtttacg ccgcaagtga tcagtacctg ggctcgctcg
     5641 agccgggggc cctggacctc gttcttgatc tgtcaaagca aggtttcggc ccgaaggcgg
     5701 tcgcctgggt cttgagcgcg ctggtggttt cgcatatgaa caacatgatc ggcgagtcgg
     5761 ccgttctcaa gggtatccag ggcgccaaag gctatccctg gtagctgtac tcccggatgg
     5821 cctgcattag aatcggccag acgtggccaa acccgtcatc ctcacggtcg acgatgaccc
     5881 ccaggtgctc aactcggtcg agcgtgacct gcgccagcac ttccgcggcg agtaccggat
     5941 cgtcaaggcc gactccgggt cccaagccct gcaggctgtc caggaactga agcgccgcaa
     6001 cgacccgctg gcgctctttc tcgtcgacca gcgcatgccg gagatgaccg gcaccgagtt
     6061 cctcactcag gccatccagc tctaccccga ggcccgcaag gtcttgttga cggcctacgc
     6121 agataccgag gccgccatcc agagcatcaa caccctgggg ctcgaccact atctgatgaa
     6181 gccctgggag ccgccggagc aaaacctctt tccggtgctg gacgatttgc tcagcgattg
     6241 gcgtgcgatg accacactgc cctacgaggg catccgagta gcgggcaccc tctggtcacc
     6301 cagcagccac gcggtcaaag atttcctggc ccgcaatcag attccttatc agtggttgga
     6361 cgtggaagcc gatgcgcagg cgagagaatt ggtgaaatcc accgatggcg gcgagccccg
     6421 cctgccagtc gtcttctttc cagacggcag cttcctggtc gagccggata acccagctct
     6481 ggctgcaaag gttggcctgc agactgaagc cagtcggcaa ttctatgacc tggtcatcgt
     6541 cggcggcggg ccggccgggc tgggcgccgc cgtatacgcc gcttcggaag gactgcgaac
     6601 cgccatggtc gagcgccact cgcctggagg tcaggccggg accagttcac tgatcgaaaa
     6661 ctaccttggc ttccccaaag gactcaccgg cgcggacctg gccaggcggg cgagtaccca
     6721 agccaaacgc ttcggcgccg aaatcatcac cgcggtggag gccgccaagg tgcgagtgga
     6781 cggaccctac cgcaccgtca cctgcaccaa cggctccgag cttaattgcc atgcgctgct
     6841 cattgccacc ggcgtcacgc tgcgcaggct ggagattccc ggggcagatg aactcaccgg
     6901 ccgaggggtc tactatggag cttctctcac agaggccgcc tactacaaag accagcatgt
     6961 ggttgtcgtc ggaggagcca attcggccgg gcagggggca atgttcttct cgctttatgc
     7021 cagcaaggtc accatgctgc tgcgagccag ttccctgagc aagagcatgt ca
//
LOCUS       DATDPQ010000365         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_461538, whole genome shotgun sequence.
ACCESSION   DATDPQ010000365 DATDPQ010000000
VERSION     DATDPQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461538"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VI520_08185"
     CDS             complement(<1..1170)
                     /locus_tag="VI520_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37896.1"
                     /translation="MFKPRLSISVVIRRLLRTTRPSGRLLWALPLAASILVGSLAAAP
                     GVPSVSAASQPDQTSTPTPGSDPAPSSSLEFTSPSGKLPARAASVAEAQASGVDLTIS
                     PNQDGWPDFNPLIMQLTLTCPAGGPNCATFLLMKIYSNDGARFWLYGAPDEPECGCGQ
                     IFEQSGPYSLRGYSAFSASVSVPAGTQRRLYWSLWIQPSSASTLFVEATWFGTNQFVI
                     ALEQMRIHPLVFIPGILGTMPPTFSGIMDPVLGIYGPLLVHLQKTGYVLDETLFPFPV
                     DWRRSILVAGQRLDEGIPGFLEQANQLDYVGEPGSGHPATKVDLVVHSMGGLITRAYV
                     EGPTYDNDIHKVVFISSPHRGFPEAYKTWEGLTWESFLYEGYHSALRILMDHYLWP"
     gene            1384..2559
                     /locus_tag="VI520_08190"
     CDS             1384..2559
                     /locus_tag="VI520_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLF37897.1"
                     /translation="MRTLRHRPAFFYAFGSLFVLAVGARSLGAYFLTGHPLRWIAASL
                     LGLFALLYSTERWASSRFPRYLHVYLAIQTVLALVILLLPPQHFFSAALGLVLSGQAV
                     LWLPGRQAYAWIGAFIGVMLFGLVQGQGLLNGLTLGLLYIGGYLFTGAYAGAVGRATA
                     AQHRSEALLSDLQEAHEQLRMYAVQAEQLAVVEERQRMARDLHDSAVQALYGLVLSSE
                     GAARKLAEGKVGLVGERLQEIRRTAHAALREMRSLIFELRPPDLEKEGLVAALRGRLE
                     SVEDRAGVRTSLEVEGDGRLPHNLEAGLYRVIQEALNNALKHSGAEQVTVSLNIGPEQ
                     VALEVADDGIGFDTQAAQSGGGLGLRGMVERAQELGGKLSLQSEPGQGTRIRLEVPQ"
     gene            2577..>2936
                     /locus_tag="VI520_08195"
     CDS             2577..>2936
                     /locus_tag="VI520_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37898.1"
                     /translation="MIADDHPVVRTGIRHLLETEPGIQLVGEATNGREAVSAVNQTRP
                     DVILLDLVMPELDGTGAIREIKVSHPDVRILVLTTFASDDKLYPAIKAGAMGYLLKDT
                     EPEDLVRAIQQVHRGEYP"
BASE COUNT          549 a          881 c          988 g          518 t
ORIGIN      
        1 cggccacagg taatggtcca tcaggatgcg cagcgcggag tggtatccct cgtaaaggaa
       61 ggattcccag gtgagacctt cccaggtctt gtaagcctcc gggaagccgc ggtggggcga
      121 ggagatgaac accaccttgt gaatatcgtt gtcgtaagtc gggccttcca cgtaggcgcg
      181 agtgatcaaa ccgcccatgc tgtgcactac cagatccacc ttcgtggcag gatggcccga
      241 gccaggctcc ccaacgtaat ccagttggtt ggcctgctct aggaatccgg gaatgccctc
      301 gtcgaggcgc tggcccgcca ccaggatgct gcgacgccaa tccaccggga agggaaacag
      361 cgtctcgtcg agcacatatc cggtcttctg cagatggacg agcaggggcc catagatgcc
      421 gaggaccggg tccatgatgc ccgagaaggt cggcggcatg gtccccagga tgccggggat
      481 gaaaaccagc gggtggatcc gcatctgctc cagggcgatc acgaactggt tggtcccaaa
      541 ccaggttgcc tccacaaaca aggtcgaggc cgaggacggt tgaatccaga gcgaccaata
      601 cagcctgcgc tgggtgccgg cggggacgga aacactggcc gagaaggccg agtacccgcg
      661 cagggagtat gggccgcttt gctcaaaaat ctgcccacaa ccgcactccg gttcatcggg
      721 tgcaccgtac agccagaagc gggcgccatc gttggagtag atcttcatga gcagaaaggt
      781 cgcgcagttc gggccgccgg cgggacaggt cagggtgagc tgcatgatca agggattgaa
      841 atccggccat ccgtcttgat tgggggagat tgtgagatcg acgccggacg cctgggcttc
      901 cgccaccgac gccgcgcgag ccggtaactt acccgacggc gaagtgaact cgagggacga
      961 gctcggcgcc gggtcagatc cgggggtggg ggtgctggtc tggtctggct gcgaagcggc
     1021 gctcacggaa gggactcccg gagcagccgc gagggagcca accaggatcg aggccgcgag
     1081 cggcagggcc cacagcaacc tgcccgaagg gcgagtggtt ctgagcagtc ttcggatcac
     1141 aaccgagatg gagaggcgcg gtttgaacat aggaggtcct ccgacctctt tattaggtgg
     1201 ttacaagcca tgcacgccgg tgtgcagggc atcggtctgt attacggcta aaaccggctt
     1261 acggaggacc ccgttccctc cgccaggagg gctccgccgc gccatactac ggccacgacg
     1321 gccagcggag atcggcaaaa ctgcgtattt ctggctccgc gccttgcacc gtataatccg
     1381 ggcgtgagga ctctgcggca caggccggcg ttcttttatg cctttgggtc gctttttgtc
     1441 ctggccgtag gagcacgttc gcttggggca tacttcctca cggggcaccc gctgaggtgg
     1501 attgcggcca gcttgctcgg cttgttcgcg ctgctctatt ccaccgagcg atgggcctcc
     1561 agtcggtttc cgcgatacct ccatgtgtat ctggccattc agaccgtcct ggcgctggtg
     1621 atcttgctcc tgccgcccca gcacttcttc tctgccgccc ttggcctggt cttgagcggg
     1681 caagccgtgc tctggctgcc cggtcgccag gcgtacgcct ggatcggcgc tttcattgga
     1741 gtcatgctct tcggtctcgt gcaggggcaa ggactgctca acggcctcac cctcggcctg
     1801 ctctacatcg gcggctacct cttcaccggc gcctacgccg gggcggtcgg gcgggccact
     1861 gccgctcaac accgaagcga ggcgctgctt tcggatttgc aggaggctca cgaacaacta
     1921 cggatgtatg ctgtccaagc cgaacaactg gcggtggtgg aggagcggca gcggatggcg
     1981 cgggacctgc acgattccgc agttcaggcg ctgtacgggt tggtgttgtc gtccgaaggg
     2041 gcggctcgaa agcttgccga ggggaaggtc gggctggtcg gcgagcggct gcaagagatc
     2101 cggcggacgg cccacgccgc gctgcgtgag atgcgatcgc tgatcttcga gctgcgacca
     2161 ccagatttag aaaaggaagg actggtcgct gccttgcggg gtcggttgga atcggtcgaa
     2221 gatcgggcgg gtgtgcgcac ctccctggaa gtcgaaggag acggccggtt gccccacaac
     2281 ctggaagccg ggctctaccg ggtcattcag gaagcgctca acaacgcgct gaaacactca
     2341 ggagcggagc aggtcacggt ctcattgaac atcgggccgg agcaggttgc gctcgaagtg
     2401 gccgacgacg ggattggctt cgacacccag gccgcccagt ccggcggcgg gctcggcctg
     2461 cgcgggatgg tcgaacgggc ccaagaactc ggcggcaagc ttagcctgca gagcgagccc
     2521 gggcagggca cccgcatccg gctggaggtg ccccaatagc cgacccgatc cgggttttga
     2581 tcgcagacga ccacccggtg gttcggaccg ggatccgcca cctcttggag accgagcctg
     2641 gcatccaact ggtgggcgaa gccacgaatg gtcgagaggc ggtgagcgcg gtcaaccaga
     2701 cccgcccgga tgtgatcctg ttggatctgg tgatgcccga gctggacggg accggggcga
     2761 tacgcgaaat caaggtcagc caccctgatg ttcgaatcct ggtgctgacc accttcgcct
     2821 cggacgacaa actctatccg gcgatcaaag ccggtgcgat gggttatctc ttgaaggaca
     2881 ccgaacccga ggatcttgtc cgggcgatcc agcaggtcca ccgcggcgag tacccg
//
LOCUS       DATDPQ010000366         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2057458, whole genome shotgun sequence.
ACCESSION   DATDPQ010000366 DATDPQ010000000
VERSION     DATDPQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057458"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1001
                     /locus_tag="VI520_08200"
     CDS             306..1001
                     /locus_tag="VI520_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HLF37899.1"
                     /translation="MRRRQPERHYGERIGWLRAAVLGANDGIVSTASLVVGVAAAEAA
                     RSSVLVAGIAGLVAGAMSMAAGEYVSVSSQADTEEADLERERNELKSDEPFERKELAA
                     IYVGRGLDVSLADQVADQLMAHDALGAHARDELGLSKIHRARPIQAALASAGTFAVGA
                     AMPLAIVVLSPPSSTIPLVAGGSLAFLALLGGIAARVGGANSLVGAARVTFWGALAMA
                     LTAAIGALFGTVV"
     gene            complement(1005..1784)
                     /locus_tag="VI520_08205"
     CDS             complement(1005..1784)
                     /locus_tag="VI520_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37900.1"
                     /translation="MTDVDQLERFAERIQGLAAGFRYPPTPDIAARLRPPRIRPIRRP
                     RLAWAIAGAMVVLAGLLAVPEVRAAVVEIVRLGAVRIFLGGPTPTPLPPTGHLSLSEL
                     PGETTLEAARASATIPIRLPSYPADLGPPNRAFILREFGGIVVLVWANPDYPDFARLA
                     LFEIPAGPIVNKLFPERIQSTSVAGRPAIWSTGDHFVELIMESRQELVLVRGNVLIWS
                     LEGVTYRLETTLSLEEAIRIAESFEEPNPPEGAPVTAEPSP"
     gene            complement(1781..>2144)
                     /locus_tag="VI520_08210"
     CDS             complement(1781..>2144)
                     /locus_tag="VI520_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF37901.1"
                     /translation="QPWLLRITANLARNRRRSIGRYWFALQRLGRPDLELRRTTEASA
                     LNREGLEALWRAVRRLSEPDQRVIYLRYFLELSEAETAAALEVAPGTVKSRLHRALAR
                     LRTIIERDFPELREGLYA"
BASE COUNT          392 a          655 c          714 g          383 t
ORIGIN      
        1 tgaagcagtt aacttcgacg ctagcaggtg ttggcacgat gtatcaatgt gtgctcaaat
       61 tgccgggcca atcctccgaa atgaagtcca tctgagcaac ttgaagcggc tactcgaggc
      121 ctagcattac gatagattcg acaagccgtc gcaccagaac caaccggcaa acgggtagtc
      181 gcccagcgtt tatgtctgcg ccccattctt ggatgcccat gcagcccgac cgagtcgtgt
      241 tagctgcctt cgtcgccgaa gctgtccttg ctggtggaga atctgcttta gctaggaagg
      301 aaaagatgcg tagacgacag cctgaacgac attacgggga gcgaattggt tggttgagag
      361 ccgccgtatt gggtgcgaac gatgggattg tctcgaccgc cagtttggtt gtgggggtgg
      421 ccgcggcaga agcggcgcgc agcagcgtgc tggtcgctgg tatcgcagga ctggttgccg
      481 gggcgatgtc gatggctgcc ggggaatacg tgtcggtcag ctcgcaggcc gacaccgagg
      541 aagccgacct tgaacgtgag cgcaacgaac taaaatcgga tgaacccttc gagcgcaagg
      601 aacttgcggc gatctacgtc ggtagggggc tcgatgtttc gctcgcggat caggtggcgg
      661 accagttgat ggcccacgat gcgctgggag cccacgcccg cgacgaactt ggtctgtcga
      721 agatacatcg agcacggccc atccaagctg cgctggcctc agccggcaca tttgcggtcg
      781 gtgcggccat gccgttggcc atcgttgtgt tgagcccgcc ctcgagcacg atcccgctgg
      841 tcgccggagg gtcgctggcg ttcttggcgc tgctgggggg gatcgccgcc cgggtgggcg
      901 gcgcgaactc actcgtcggc gcggcgcggg tgaccttctg gggagccctg gccatggccc
      961 taaccgcggc gattggagcc ctgttcggca cagttgtctg aggcttaagg cgagggctcg
     1021 gcggtgactg gagcaccctc gggcgggttg ggttcctcga acgactcggc gatccggatc
     1081 gcttcttcca aggatagagt cgtctccagc cggtacgtga ccccctcgag ggaccagatc
     1141 aacacgttgc ctcggaccag caccagttcc tgacgacttt ccatgatcaa ctcgacgaag
     1201 tggtcgccgg tcgaccagat cgcgggccga ccggccacgc tcgtcgactg gatgcgctcc
     1261 gggaaaagct tgttcacgat cggcccggcc gggatctcga aaagtgccag gcgagcgaaa
     1321 tcggggtagt cgggattcgc ccaaaccagg acgacgattc ccccgaactc tctcaatatg
     1381 aaggcgcggt taggtgggcc gaggtcggcc gggtaactcg gcagccgaat cgggatggtg
     1441 gccgaggccc gggctgcctc tagcgtggtt tcaccaggca gctcgctcag gctcaaatgt
     1501 ccggtcggcg gaagcggggt cggagtcggg ccgccaagaa agatccggac agcaccgagc
     1561 cgcacgatct cgaccaccgc ggcccgcact tctgggacgg ccagcagccc ggccaggacc
     1621 accatggccc ccgcgatcgc ccaagccagc cgaggccgtc ggattggtcg gatccgcggc
     1681 ggtcgcagcc tggcagcgat atccggcgtc gggggatagc ggaatccggc ggctagcccc
     1741 tggattcgtt ccgcaaacct ctccaactga tcgacgtcgg tcatgcgtaa agcccttccc
     1801 gcagttctgg gaaatctcgc tcgatgatgg tgcgcagccg ggccagtgct cggtgcagcc
     1861 gagatttgac ggtccccggc gccacttcca gggcggcagc cgtttccgcc tccgagagct
     1921 ccaagaagta gcgcaggtag atcactcgct gatcaggctc gctcaggcgg cgaacggccc
     1981 gccacaatgc ctcaagccct tcacggttca gagccgacgc ttccgtggtc cgccggagtt
     2041 ccaggtccgg ccgcccgagt cgctgtaagg cgaaccagta acggccaatc gatcggcgcc
     2101 gattgcgggc caggttggcc gtgatccgca gcaaccaggg ctgc
//
LOCUS       DATDPQ010000367         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2060504, whole genome shotgun sequence.
ACCESSION   DATDPQ010000367 DATDPQ010000000
VERSION     DATDPQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060504"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VI520_08215"
     CDS             <1..1279
                     /locus_tag="VI520_08215"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HLF37902.1"
                     /translation="SLRDGLTSQELQELWTEGSGVFVGTPDAEALAGLLGEASAQIMP
                     AETLLDAAWSAGTAYAIVPFEALEPRWKVLEIDGLSPVRAQFDSQNYQLAIDFGLSGD
                     PAGMDAVQSALVWPASNRDPDQLTVVVMTGVTALVRATAWRMDRYGPDYPGLQIGDWL
                     READVAHVSHEASFNPNCPPPEPFSASLRFCADPDYVNLFTGIGVDLVELTGNHLQDY
                     GPDPLRFTLDLYHQLGFQTFGGGEDLLRARQPAIVEHNGNRLAFLGCNAAGPRGDWAA
                     ERSPGALACQDEGLLQHVAELTAQGYLVVFTFQWPESMSSYPLQAQIESFRTAAEAGA
                     VIVNGSQAHRPQTMEFYAGSFIHYGLGNLFFDQMHSLPLRQEFLDRHVFYAGRHVSIE
                     LLTALLEDYAQPRPMNPQERAELLAEIFEVSGW"
     gene            complement(1312..>2776)
                     /gene="ftcD"
                     /locus_tag="VI520_08220"
     CDS             complement(1312..>2776)
                     /gene="ftcD"
                     /locus_tag="VI520_08220"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HLF37903.1"
                     /translation="DVIEAIENAIRSVEGIQVLDRHVDADHNRSVITFVGSPAAAAEA
                     AFAAIAKAAELISLETHQGEHPRIGATDVVPFIPVREVSMRECVDLARALGSRVAQQL
                     GIPVYLYEEAAARPDRVNLEDIRRGEYEGLRSSIVSDPERKPDFGPQQIGPAGATVIG
                     ARRPLIAYNVYLTTDDVEVAKRIAKTVRFSGGGLRFVKALGLLVDGRAQVSMNLTDFT
                     RTPIHRVVELIRREADRYGVGIHHSELVGLIPQEALVESARWYLQLDALEPAQVLENR
                     LFDSAAPGADRFLDQLAAGTPTPGGGSAAAYAGAMAAGLVAMVGRLTLGKKKYAAVQH
                     QVEVIVATADELRSRLEAAVTQDARAFEAVLEANRLPADSEQSKRARMAAIEQATLPA
                     GEVPLGVARNCAEVLALALEIASVGNVNAISDASVAGDLALAGLSGAALNVRVNAAGL
                     LDPEPGEGWLAELETVQASAQAALGQLRGVLTERAGL"
BASE COUNT          483 a          900 c          889 g          504 t
ORIGIN      
        1 cagcctcaga gatgggttga cttcccagga gttgcaggaa ctgtggaccg aggggtcggg
       61 cgtgttcgtc ggaacgccag acgccgaggc attagcaggc ctccttgggg aggcttcggc
      121 tcagatcatg ccggccgaaa ctttgctcga cgcggcgtgg agcgccggta ccgcctatgc
      181 gatcgtgccc ttcgaagcgc tggaaccccg ctggaaggtg ctggagatcg atgggctatc
      241 gccggtgcgt gcgcagtttg attcacagaa ctatcaactg gccatcgact tcgggttgag
      301 cggcgacccg gccgggatgg atgcggtcca gagcgccttg gtctggcctg ccagcaatcg
      361 ggatccggat cagttgacgg tcgtggtcat gaccggagtg acggcactgg tccgggcgac
      421 ggcctggcgg atggatcgct atggccccga ctatccggga ctgcagattg gtgactggct
      481 gcgcgaggcg gatgtcgccc acgtcagcca cgaagcgtcc ttcaatccga actgcccgcc
      541 gcccgaaccg ttctcagcga gcctgaggtt ttgcgccgac cccgattacg tgaacctgtt
      601 cactgggatc ggggtggatc tggtcgaact gaccgggaac catctgcaag actatggccc
      661 ggatccgttg cgctttaccc tcgatctcta tcatcagctc ggatttcaga ccttcggagg
      721 tggggaggac ctgctcaggg cccgacaacc ggcgatcgtc gaacacaacg gcaaccgact
      781 cgccttcctg ggttgcaatg cggccggccc gcggggagac tgggcggccg agcgcagccc
      841 gggcgcgctg gcctgccagg acgaagggct cctgcagcat gtcgccgagc tgaccgccca
      901 aggttacctg gtggtgttca cctttcagtg gccagaatcc atgagttctt atccgctgca
      961 agcccaaatc gagagcttcc gcaccgcggc ggaggctggc gcagtgatcg tcaatgggag
     1021 ccaggcccac cggcctcaga ccatggagtt ctacgccggc agcttcattc attatggtct
     1081 gggcaacctc ttcttcgatc aaatgcactc gctccccctg cggcaggaat tcctggaccg
     1141 gcacgtgttc tatgccgggc ggcatgtcag catcgagctg ctgaccgccc tgctcgaaga
     1201 ctacgcccag ccgcgcccaa tgaacccgca ggagcgggcg gaactgctcg ccgagatctt
     1261 tgaggttagc gggtggtagc gggcggacga tccgatttcc gcgggccgga gctagaggcc
     1321 ggcgcgctcg gtgagcacgc cccgcagttg gccgagagcg gcctgagccg aggcttgcac
     1381 tgtctctaac tcggcgagcc aaccctcgcc gggttcggga tccagtaatc cagcggcgtt
     1441 gacccgtacg ttcagcgcgg cgccgctcag gccggccagc gccaggtctc cggcgacgct
     1501 ggcgtcgctg atcgcgttta cgtttccgac cgaggcgatt tcaagcgcca gggccaggac
     1561 ttcggcgcag ttccgggcga cccccaacgg aacctccccg gcaggcagtg tggcctgctc
     1621 aatcgccgcc atgcgggccc gcttcgattg ctccgaatcc gccggaagcc gattcgcctc
     1681 cagtactgct tcgaatgctc gggcgtcctg ggtgaccgcg gcctccagtc ggctgcgcag
     1741 ctcatcggcc gtggccacga tcacctcgac ctggtgctga accgcggcgt attttttctt
     1801 gcccagtgtc agccgcccca ccattgcgac caacccggct gccatcgctc cggcgtaggc
     1861 ggccgccgag ccaccgcccg gggtgggagt tccggcagcc agctgatcga ggaagcggtc
     1921 ggctcccggc gcggccgaat cgaacaagcg gttctccaag acctgagccg gttctaaggc
     1981 atccaactgc agataccagc gggcggactc gaccagcgcc tcttgcggaa tcagaccgac
     2041 cagctcggaa tggtgaatgc caactccgta gcgatccgct tcgcgtcgga tcagctccac
     2101 gacccggtgg atgggggtac gagtgaaatc ggtcaggttc atggagacct gagcccgtcc
     2161 gtcgaccagc agtcccagcg ccttgacgaa gcgcagccct ccaccagaga accggacggt
     2221 tttggcgatt cgcttcgcaa cctccacatc gtcggtagtg agataaacgt tgtaggcgat
     2281 caacggtcgc cgggccccga tgacggtcgc cccggccggg ccaatctgct gtgggccgaa
     2341 gtctggcttt cgctcggggt ctgaaacaat ggaagatctg agcccctcat actctccccg
     2401 gcggatatct tccaaattga cccgatcggg ccgagccgca gcctcttcat acaagtagac
     2461 cgggatcccg agctgctgag ctacccggct tcctagggcg cgagcgagat ccacgcattc
     2521 ccgcatgctg acctcgcgga ccgggatgaa gggcacgacg tcggtggctc cgatccgggg
     2581 gtgctctcct tgatgcgtct ccaaactgat cagctcggcg gccttggcga tagcagcaaa
     2641 ggccgcctcg gccgcggccg ccggagatcc gacgaacgta atgacgctgc gattgtgatc
     2701 ggcatcgacg tgccggtcca acacctggat tccttcgacc gagcggatcg cgttttcgat
     2761 ggcctcgatg acatcg
//
LOCUS       DATDPQ010000368         3117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2838055, whole genome shotgun sequence.
ACCESSION   DATDPQ010000368 DATDPQ010000000
VERSION     DATDPQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2838055"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..774)
                     /gene="rpe"
                     /locus_tag="VI520_08225"
     CDS             complement(91..774)
                     /gene="rpe"
                     /locus_tag="VI520_08225"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLF37904.1"
                     /translation="MPAEYLLAPSILAADFLRLGQAIQEVEAAGADWIHIDVMDGLFV
                     PNISMGPIVVRAARRATGLPLDVHLMITDPVRYLEAFSEAGADSLTVHVEAAGHLHRT
                     LEKIHELGLRAGVALKPETPAGQVYEVLPLLDLVLVMTVEPGFAGQSFIPETLRKVEQ
                     FRDWQHAGLTRARIEVDGGITAETAPLAARAGAEVFVAASAIFEHAGGVGAGVQAILE
                     SLELNPIQE"
     gene            992..2245
                     /locus_tag="VI520_08230"
     CDS             992..2245
                     /locus_tag="VI520_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLF37905.1"
                     /translation="MKRRRSDPPPVDERSLRLEQEVQKLRTVLRGTATLNSALNYERV
                     MDLALDLATSALADVDSSAGQLVSALLMYDGDTLQLVSSRGFLHADQNAVIPGRIGVV
                     EQAMSSGQLQVTEDPAGDPELGRLLALRRCSIAVCIPLVVGLEVYGIMLFGHPRADEL
                     NEEGIELVEAIAQQAVVALQNSRLYGQLEQEKERMAEIQEEARHKLARDLHDGPAQSI
                     GAIAMRVNFARRLIARDPKSASDELFKIEELARRTSKEIRQMLFTLRPLILESEGLLA
                     ALKDLSRKFEEMHHQKVHIDVDPQIEAAMEVGKQGVVFFLVEEAVNNARKHAKAEHIW
                     VRLKRTGDLARLEIEDNGAGFDVSAVQDNYEQRGSLGMVNLHERSEMVNGTLKIESVP
                     GRGTRISVEIPMTIEAAERRHRIGG"
     gene            complement(2256..>3117)
                     /locus_tag="VI520_08235"
     CDS             complement(2256..>3117)
                     /locus_tag="VI520_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HLF37906.1"
                     /translation="LLPERAIMRGAEFSAPFSLRTRMIEPRDLQGWVIDMDGVLWRGE
                     TPLPGLQEFFGLLRDRKLGFILATNNARQTAERVVAKLASFGVSIHPEQVLTSSVGAA
                     AHLQSQLPAGSAVFAIGEQVLIEALEAAGFPVLPTAKGARAVVVGIDSELSWKKLAEA
                     AYALENGALFLGTNPDVSFPTERGEAPGNGATLAALTATTGRQPMVFGKPEPHLFRQA
                     IARLGTRPERTASLGDRLETDILGGSRAGLRTILVLTGVTSRSDLAGAEVQPDWVYED
                     LVELWRALQG"
BASE COUNT          597 a          956 c         1017 g          547 t
ORIGIN      
        1 ggacgttctg tgcaaagtgc atcaagcgcc ttgggcgggc ctgagatctt cggttcgaaa
       61 tcagattgag accgccctcg ggcggtcttt tcattcttga atcggattca gttcaagcga
      121 ctccagaatg gcttgaactc cggcaccaac gccgccggca tgctcgaaga ttgcgctggc
      181 cgcgacgaac acttcggccc cggccctggc cgccagtggg gcggtctcgg cggttattcc
      241 cccgtctacc tcgatccggg cccgggtgag ccctgcgtgc tgccaatcgc ggaactgctc
      301 aaccttccgc agggtttccg ggatgaaaga ttgacccgcg aaaccaggct cgaccgtcat
      361 gaccagcacc aggtccaaga gcggcaacac ttcgtagacc tgacccgccg gggtctccgg
      421 cttcagggca accccggccc tcagacccaa ctcgtggatc ttttccagcg ttcgatgcag
      481 gtggccggcg gcctcgacat gtaccgtgag cgagtcggcc ccggcttcgc tgaaggcttc
      541 caggtagcga accggatcgg tgatcatcaa gtggacgtcg agtggaagac cggtcgcccg
      601 ccgggccgcc cgcacgacga tcgggcccat actgatgttc ggcacgaaga gcccgtccat
      661 cacgtcgatg tggatccagt ccgccccggc ggcttccact tcctggatgg cttggccgag
      721 ccgcaggaag tctgcggcta gaatcgaagg cgcaagcagg tattcagctg gcatcggtga
      781 gaatcatacc tgtcgggtat gcggttgaca agcaaatggg aagtgaggcg gctcgaaagc
      841 ggcgccggga gggccggcgt aggaatccgg ctgcatccgc ggtttgtagg gcgactgtaa
      901 ggcgatcaag tgcggcttgc gccgatactg gaccttgggg accgctgtgg aggccctggg
      961 gtttcgcttt ggcgatcatg aatccaactc gatgaaaagg cgccgctcag atccaccgcc
     1021 ggtcgacgag cgcagcctcc ggcttgagca ggaagtccag aagctcagga cggtgctgcg
     1081 cggaaccgcg actctgaatt cggccctcaa ctacgagcga gtgatggatc tagcgctcga
     1141 tctggcaacc agcgccctgg cggatgtgga ttcctcggcc gggcagctgg tcagcgcgct
     1201 gctcatgtac gacggcgaca cgcttcagtt ggtttcttcg cgtggcttcc tacacgccga
     1261 tcagaacgcg gtgatccccg gccggatcgg agtcgtcgag caggcgatgt cgagcggcca
     1321 gctgcaggtc acagaagacc ccgccggtga cccggaactg gggaggctgt tggcactgcg
     1381 ccgctgcagc attgcggtgt gcattccgct ggtcgtcggc ctggaggtgt atgggatcat
     1441 gctgttcggt caccctcggg ccgatgaatt gaatgaagag gggatcgagc tggtagaggc
     1501 gatcgcccag caagccgtcg tggcgctgca gaactcgcgc ttgtatgggc agctcgagca
     1561 ggagaaggag cggatggcgg agatccagga agaagctcgc cacaaactgg cccgcgacct
     1621 gcatgacggc ccggcccaat cgatcggggc gatcgcgatg cgggtgaatt tcgcccgccg
     1681 cttgatcgcg cgggatccca agtccgcctc cgacgaactc ttcaagattg aggaattggc
     1741 ccgccggacc tcgaaagaga ttcggcagat gttgttcacc ctgcggccgc tcatccttga
     1801 gtcggagggg ttgctcgcgg cgctgaagga cctatcccgc aagttcgagg agatgcatca
     1861 ccaaaaggtc catatcgacg tcgatcccca gatcgaggct gccatggagg tcgggaagca
     1921 aggggtggtg ttcttcttgg tggaagaggc ggtcaacaat gcccgcaagc atgccaaggc
     1981 tgagcacatc tgggtccggc taaaaaggac cggtgacctg gccaggctgg agatcgaaga
     2041 caacggagcc gggttcgacg tgagcgcggt gcaggacaac tacgagcagc gcggcagcct
     2101 ggggatggtc aacctacatg agcggtcgga gatggtcaac gggaccctga agatcgaatc
     2161 agtccccggg cggggcaccc ggatatcagt tgaaattccg atgacgatcg aggcggccga
     2221 gcgccggcac cggatcggcg gttgagctca aggcctcagc cctgcagggc ccgccagagt
     2281 tcaaccaaat cctcatacac ccaatccggc tggacttcag cgccggctag atccgaacgg
     2341 gaagtgaccc cggtcaatac caggatggtc ctgagcccgg cccggctgcc tcccaggata
     2401 tcggtctcca atcggtcccc cagggaagcc gtccgctcgg gccgtgtccc cagtcgggcg
     2461 atcgcctgcc tgaacagatg gggctcgggt ttgccgaaca ccatcggctg cctgccggtg
     2521 gtcgcggtca gcgccgccag agtcgcccca ttcccgggag cctcgcctcg ctcggtgggg
     2581 aaagaaacgt ccgggttggt gccaagaaac aaggcaccgt tctccagcgc gtaggccgcc
     2641 tcggccagtt tcttccagct cagctccgaa tcgattccaa ccacgaccgc ccgggcacct
     2701 ttcgcagtcg ggaggaccgg aaacccggct gcttcaaggg cctcaatcaa cacctgctcg
     2761 ccgatggcga acacggcgga tccggcggga agttgggatt gcaggtgggc ggccgcgcca
     2821 accgagctgg tgagcacctg ctcgggatgg atcgacaccc caaaggaggc cagcttggcc
     2881 accactcgct ccgcggtctg gcgggcgttg ttggtcgcca ggatgaatcc aagctttcgg
     2941 tcgcggagca acccaaagaa ttcctgcaag ccggggagcg gagtctcccc ccgccagagg
     3001 actccgtcca tgtcaattac ccatccctgc aggtctcgcg gctcgatcat tctggtccgt
     3061 aagctgaacg gggcgctgaa ttcagcgccc cgcatgattg ctcgctctgg taagagt
//
LOCUS       DATDPQ010000369         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2329673, whole genome shotgun sequence.
ACCESSION   DATDPQ010000369 DATDPQ010000000
VERSION     DATDPQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329673"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="VI520_08240"
     CDS             <1..2026
                     /locus_tag="VI520_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF37907.1"
                     /translation="WLRYNPPAMGARLSALGLSLLIAACASGAVVPPRSDSPAERLVR
                     QADGRLVVSDFEGAIATYRQAIEADPRFAHAYSRLAYAQLFNPASQAEAIEQASLATE
                     HQPDSGRAWAYLGRALDWNGNFDEALLAAERAVELSPDDPEVQSFLAEILADLRMYDE
                     ALVAAEKAVRLGPENAEAHRNLAYVYEFVGRREEALDEYLRAHELESQFVYRHSSLAG
                     YYLYEVDEQLTGRQWLESAQQLAPDDYITLLLLARLYSDRGELDQAIANCERILELAP
                     AASDGHDCLGGVYLDAGRYQDAEAERQAAIAADPENETGYLGLGYVYSARQACQEALV
                     QFNTAVELHPRTGTNHAAVGFAYACLGEWEQAEVAYQQAIDLEPYEGDHHILLGRMYL
                     ERGDPDRAEPEFKKAVDLDPANDQYVAWLGRLYSSQGELEQAVAEYERAAAMDPDDAS
                     HELSIGFAYLGAGADPDQAEPRFQRALDIYLEQGATAREIAQATYGLALVQLARADCA
                     GALPQLQEAVRLDGALTAARDYLLQCRQVAGFSGANLPPELAQRSQYAGRDALDALRL
                     GLAAVGIEARAEFRPGEGGENLLLVAHAAISEPGSAEFLTQQSLVTFAGSQVAARLLV
                     PTVDRLLVISLNPEGDSVGLLEVSVRDARLWELGVLNDAQFTSFWRSLNP"
BASE COUNT          386 a          651 c          671 g          392 t
ORIGIN      
        1 ctggctccgc tataatccac ccgccatggg tgcgagactc agtgcgctcg gtctctccct
       61 cctgattgcc gcctgcgcta gtggagccgt tgtaccgccg cgatccgact cgcccgcgga
      121 gagactggtt cggcaggcgg atgggcggct cgtcgtcagt gatttcgaag gagcgattgc
      181 cacctatcgg caggcgatcg aggcggaccc gcgatttgcc cacgcctata gccggctggc
      241 atatgcccaa ctcttcaatc ccgcctccca agccgaggcg atcgagcagg ccagcttggc
      301 caccgaacac cagcccgact cggggcgagc ctgggcctac ttgggcaggg ccctggattg
      361 gaacggcaac ttcgacgagg cgctgcttgc ggcggagcgg gcggtggagc tcagtcccga
      421 tgacccggag gttcaaagtt tcctcgctga gatcctggcc gatctgcgaa tgtacgacga
      481 ggcgctggta gcggccgaga aggcagtcag gctcggccct gagaatgcag aggcccaccg
      541 taacctggca tacgtctatg aattcgtggg gcggcgggaa gaggcgctcg acgaatacct
      601 tcgtgcccac gagctggaat cccaattcgt ttaccggcat agctcgttgg caggctacta
      661 tctatacgag gtcgacgaac agctgaccgg ccggcagtgg ttggaatcgg ctcagcaatt
      721 ggcaccggac gactacatca ccttgctgtt gctggcccgc ctgtacagcg atcgtggcga
      781 actcgatcaa gccatcgcca actgtgaacg aatcctggag ttggcgccgg ccgcctccga
      841 tggccacgac tgcctgggcg gggtgtatct ggatgccggc cgctatcagg atgccgaggc
      901 cgaacgtcag gccgcgatcg cagccgatcc ggaaaacgaa accgggtatc tcggcctggg
      961 gtatgtctac tccgctcggc aggcctgcca ggaagcgctc gtccagttca acacggcggt
     1021 cgagcttcac ccccgtaccg ggaccaatca cgccgcagta ggcttcgcct atgcctgcct
     1081 cggagagtgg gagcaggcag aggttgctta tcagcaagcc atcgatctgg aaccctatga
     1141 aggcgaccat cacattctgc tcggaagaat gtatctcgaa cgtggcgatc cagaccgagc
     1201 cgagcccgaa ttcaagaagg cagtcgatct ggatcccgcg aacgatcagt atgtggcctg
     1261 gcttggccgg ctgtattcct cccagggtga gctggagcag gccgtggctg aatatgaacg
     1321 ggcggcagcc atggatccgg acgacgcctc gcatgaactg agtatcggat tcgcctacct
     1381 gggagcggga gccgatccgg accaggccga acctcggttt caacgggcgc tggacatcta
     1441 cctcgagcaa ggcgcaaccg cgagagagat cgctcaagcc acgtacggac tggcgctcgt
     1501 tcagcttgcc cgggccgatt gtgccggcgc cctcccacaa ttgcaggaag cggtccgctt
     1561 agacggtgcg ctgactgcgg cccgcgatta ccttctgcag tgccgtcagg tggcgggatt
     1621 ctcgggtgcg aacttgcccc ccgaacttgc ccaacgcagc caatacgccg gcagagatgc
     1681 actggacgcg ctccggctgg gtctggcggc ggtcggaatc gaggcccgag cagagttccg
     1741 acctggggag ggtggggaga acttgctgct ggtggctcat gcggccatca gcgagcccgg
     1801 atccgcagag ttcttaactc aacaaagcct ggtgactttt gccggctcgc aggtggccgc
     1861 tcgtttgctg gtcccgaccg tagatcgatt gctcgtgatc tcgctcaacc cggagggtga
     1921 ctcggtcggg ctcttggaag tcagtgtgcg ggatgcccga ttgtgggagc tcggagtgct
     1981 gaacgatgcc cagttcacct ccttctggcg cagcctcaac ccctaggcgt ttccaaccgg
     2041 gttcctccgc tttaccatgc gcgcaatact ggcgctaagc cgcttgccta ccctttgcgg
//
LOCUS       DATDPQ010000370         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1785330, whole genome shotgun sequence.
ACCESSION   DATDPQ010000370 DATDPQ010000000
VERSION     DATDPQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785330"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /gene="sppA"
                     /locus_tag="VI520_08245"
     CDS             <1..1514
                     /gene="sppA"
                     /locus_tag="VI520_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HLF37908.1"
                     /translation="DRRVLGVVLLLRDLEPPQAGLESLAELVAELREAGKRVVTWASH
                     YSAASYQIAAGTDEILLQEDGSIDPLGISRTYSFLGETLQRIGLQADFLAIAPHKSAP
                     DTFTRKDMSAEAREMATWLMDSAYQERIDALAAGRGIGRAEAEALVDASPFSDLSALE
                     IGAVDGLLSEEQLPTHLGGEHGQARLKSWEAARSTLRRLPPIRSGQYIALIPVEGTIM
                     NGESGRPPFRPPIPVPILLDARAGDLTVVQAARAALADRRAAAVVVYVNSPGGVSSAS
                     EAMRSALARIEPTKPLIISMGPLAASGGYMVATAGRHIFAQPSTITGSIGVFSGKLAA
                     GGFLDRVLVGRETISRGRHSRIFDLERPFSSEERELVLGFVRRSYQVFLETVARSREL
                     AVETVDAIGGGRVWTGRQALEKGLVDELGGLDRAVSRARQLAGLPANAPLVLIQPGKQ
                     SIAPSSSIAAFLGSLLAGTLPEHSLGATLDYVVEGLRLFNRSKSHSMLLLVTE"
     gene            1541..>2640
                     /gene="amrB"
                     /locus_tag="VI520_08250"
     CDS             1541..>2640
                     /gene="amrB"
                     /locus_tag="VI520_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HLF37909.1"
                     /translation="MAGTWYPDEADELATSVDGYLAGVNRLDGSPLGLIVPHAGYRYS
                     GAIAAAGFKQLSGVKIDMAVVIAADHQPPLSRPISVWSRGGFETPLGVLPVDERTAER
                     LIAYDPRIQFDPDSHAFEHPIEIELPFLKRVLPDCRIVPILMMSDADDETVDLLAEAL
                     LAVLPEAGVVLIASSDLSHYPSYDDAVRIDRATLGAIELGDPRLVRQAIRRTMEGGVH
                     GLATCACGEAPILVTMKVAQARGATDVTLLDYANSGDVEVDRRSQVVGYGAVMFWRYQ
                     PPRLSLAQQQLLLRVARTAAEGQVRKLPAPEAAAEDPVLRRLSAVFVTLRVRGELRGC
                     IGQLRADTELYQAVAEKSIAAASADPRFPSLGA"
BASE COUNT          445 a          822 c          879 g          494 t
ORIGIN      
        1 cggatcgccg ggtgctgggg gtcgtgctgc tactgcgcga tctggaacct ccccaggccg
       61 gcctcgaatc gctcgcggag ctagtcgccg aacttcggga agccggcaag cgagtggtca
      121 cgtgggcgag ccactattcc gccgcaagct accagatcgc ggcgggaacc gacgaaattc
      181 tgcttcaaga agatggctcg atcgatcccc tcggaatttc caggacgtac tccttcctgg
      241 gcgaaactct ccaaagaatt ggcctgcagg ccgacttcct tgccatcgcc cctcataaat
      301 ccgcgccgga cactttcacg cgcaaggaca tgtccgccga agcccgagag atggcgacct
      361 ggctcatgga ttccgcctat caggagcgga ttgacgccct ggccgccggc cgagggatcg
      421 gccgggctga ggcagaagcg ctcgtggatg ccagcccgtt ctccgatctg agcgcgctgg
      481 aaatcggggc ggtagatggg ctgctcagcg aggagcagct cccaacccat ttgggcggag
      541 agcacggcca ggctcggctg aagagctggg aggcagcccg ttccacgctg cgccggctgc
      601 ctccgatccg ctccggtcaa tacatcgcct tgatcccggt cgaaggcacg atcatgaatg
      661 gagagagtgg gcgccctccc ttccggcctc caatcccggt cccaattctg ctggatgcgc
      721 gggccggaga cctgaccgtc gtacaggcgg cccgagcggc tctggccgat cggcgcgccg
      781 cggccgtggt ggtgtatgtc aactcgcccg gaggagtttc gtcggcctct gaagcgatgc
      841 gctccgcgct ggctcggatc gaaccgacca agcccttgat catcagcatg gggccgctgg
      901 ctgcctccgg cgggtatatg gttgcgaccg ccgggcgaca catctttgcc cagcccagca
      961 cgatcaccgg atcgatcggc gttttctctg gcaagcttgc cgcaggtgga tttctggacc
     1021 gagtgctggt cgggagagaa actatctccc ggggccggca ctcgcggatc tttgatttgg
     1081 agcgaccgtt ctcttccgag gagcgagagc tggttttggg gttcgttcgg cgaagctacc
     1141 aggtgtttct ggaaacggta gccaggagcc gggagctggc agtcgagacg gttgatgcca
     1201 tcggcggtgg gcgggtgtgg accggccgac aggcgctgga aaagggattg gtcgatgaac
     1261 ttggcgggct ggatcgtgcg gtgagtcggg cccgccagct ggctggattg cccgcgaatg
     1321 cgccgctcgt gctcatccaa cccgggaagc agtcgatcgc tccctccagc tcgatcgcag
     1381 cgttcctggg cagcttgctg gctggcactt tgcccgaaca ctccttgggt gcgaccctgg
     1441 actacgtcgt agaagggctc cgcttgttca atcgatcgaa atcgcactcc atgctgctac
     1501 tggtgaccga atagcgacca cctggattcg gccgccggcc gtggccggca cctggtaccc
     1561 ggatgaggcc gacgaactgg cgaccagcgt ggatggctat ctggcgggag tcaatcggct
     1621 ggacgggtcg cccctgggtc tgatcgtccc ccacgccggt taccgttact cgggtgcgat
     1681 tgcggccgct ggattcaagc agctgagcgg ggtgaaaatt gacatggcgg tcgtgatcgc
     1741 cgccgaccat cagccgccgc tgtcccgccc gatttcagtg tggtcgaggg gtgggttcga
     1801 gaccccgctc ggagtgcttc cagtagacga acggaccgcc gagcggttga ttgcatacga
     1861 tccgcggatc caattcgacc cggacagcca tgcgttcgag cacccgatcg agattgagct
     1921 tcccttcctc aaacgagtgc tgcccgattg tcggatcgtg ccaatcttga tgatgagcga
     1981 cgcggatgat gaaaccgtcg atctgttggc agaggccttg ctcgccgttc tgccggaagc
     2041 cggggttgtg cttatcgcca gctcagatct gtcgcattac ccatcctacg acgatgcggt
     2101 tcggatcgat cgggccaccc tgggagccat cgagcttggc gatcctcgat tggtcaggca
     2161 agcgattcgg cgcacgatgg agggtggtgt ccacggcctg gccacttgtg cctgcggcga
     2221 agccccgatc ctggtgacga tgaaggtcgc ccaggcccgt ggcgccacgg acgtaaccct
     2281 gcttgactac gccaactccg gagacgtcga ggtcgatcgg cggagccaag tcgtcggtta
     2341 cggggcggtg atgttctggc gctaccaacc tcccaggctg agtctcgccc aacagcagtt
     2401 gctcctgagg gtcgcccgga cggcggccga aggtcaggta agaaagttgc ccgcccccga
     2461 ggccgctgcc gaggatcccg tgctgcggcg gctaagtgca gtgttcgtga ccctccgggt
     2521 gcgcggcgag ctgcgaggat gcatcggcca gttgagggcc gacaccgagc tctaccaggc
     2581 ggtcgcagag aaatccattg cggcggcctc ggccgaccca cgcttcccgt cgctcggcgc
//
LOCUS       DATDPQ010000371         7395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2063457, whole genome shotgun sequence.
ACCESSION   DATDPQ010000371 DATDPQ010000000
VERSION     DATDPQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063457"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VI520_08255"
     CDS             <1..635
                     /locus_tag="VI520_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HLF37910.1"
                     /translation="KYPEKWELAQITNGEGRTGGIREALQGADVVIALSKPGPGVIVP
                     DDIRLMGPDPIVFACANPIPEIWPWEAAEAGARVVATGRSDFPNQVNNSLGFPGIFRG
                     VLDVRSTRITDEMCIAAAEELALIAQEKGLEANSILPSMSEWEVFPRVATAVGMKAQE
                     QGLAQVQVARADLYQRAHDRIQRARAIVASDMERGFIKEPPSEETVIYRR"
     gene            complement(749..928)
                     /locus_tag="VI520_08260"
     CDS             complement(749..928)
                     /locus_tag="VI520_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37911.1"
                     /translation="MMLQEATPDTLGYMLFGYGVILGTMALFALSLVSRFRNLRRDLE
                     LMEEVQAPKRTTTES"
     gene            complement(975..1676)
                     /gene="ccsA"
                     /locus_tag="VI520_08265"
     CDS             complement(975..1676)
                     /gene="ccsA"
                     /locus_tag="VI520_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLF37912.1"
                     /translation="MQPMPNNLRVLTGVTIALLVGALYLVFGYAPREVIMGEVQRVFY
                     FHVAAGWVGGLAFLLTAVAGAIYLARRKRHWDLLAMASVEIGIVFTFINIASGSIWAR
                     PAWNTWWTWDPRLVTATIMELLYVAYLMLRQGIDDPDRRARFGSVYGIAAFVSVPITF
                     LSIRFFRTIHPVVIGSSDPSAEGAFDMTPRMLQTFLFSLLTFTFVYITLLWHRVRLGR
                     LAEQVEERKMEMLTA"
     gene            complement(1708..2430)
                     /locus_tag="VI520_08270"
     CDS             complement(1708..2430)
                     /locus_tag="VI520_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLF37913.1"
                     /translation="MLERAEAKLRAPAPGVFASYLRAVGAVIWKDLTAELRSRELLGA
                     MLVFGLLVILIFNFALELDVRARQSVTAGVLWVTFAFAGTLGLNRAMAVERERGCLDG
                     LLLAPVDRSALFFGKALGNLLFMLIVAAIILPIYSLLYNVNLFRPALAGVIVLGSIGY
                     TTVGTLFSAMAVQARTRDILLPILLFPVVLPLLIAAVRATSSLLLAIPLTEIRGWFNL
                     LIVYDLLFVAVAIMTFDYVVEE"
     gene            complement(2423..3121)
                     /gene="ccmA"
                     /locus_tag="VI520_08275"
     CDS             complement(2423..3121)
                     /gene="ccmA"
                     /locus_tag="VI520_08275"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HLF37914.1"
                     /translation="MIEVRGLTKAYGLRPVLRKLDLAVKQGEFVAVVGPNGAGKTTLL
                     RILASLARPTFGEVRLAGLRLPGQAAAARRILGVVSHQPLLYGELTAQENLRFYGRMY
                     GVADLEARIEEVLGIVGLNSRRHDLVRKYSRGMQQRLAIGRGILHDPELLLFDEPYTG
                     LDPEAAEALDGLLTQVSSEGRTVLMTSHDLVRAAGLASRVDILFGGVIARSVRRGELP
                     AADLANAYRQVTHA"
     gene            complement(3114..4607)
                     /locus_tag="VI520_08280"
     CDS             complement(3114..4607)
                     /locus_tag="VI520_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLF37915.1"
                     /translation="MRFAKGRLLPAALLGLLSAACSLAGDVTPPPALATAQMARPLPT
                     IVATGEVIPPDGPPDLTAGAAIYLDKCAACHGVGGLGDGELAANLEFPPAPLGDPSFA
                     RAARPQDWYAVVTLGRIDRLMPGFVSLSDQERWDVVGYALSLSSSLEPVALADSSAPA
                     RVTGTVRTGTAAAVVPAGLQINLFGFDGEQEALRQTVVADRQGGYRFDEVEAVPGRLF
                     FTTVEYQGVIFRSELAHVPADAGQLQLPLTIYETTSDAGALAVERLHVLIDFPAEDLI
                     RVLELWVLANPSDRVIVSPLNIPLPPGAVNLTFEQGELGERFELTELGFLDREPIPPG
                     SGIDQLAFAFDLPAAARFEQQVQQPVQAVTVLVPAEGARVSGLEDRGVQDLGGLPMRN
                     YIGGPLDPGEVLSFRVSGAVGSSPALESTLIGAAALVGAGLLTIRWWTRGKPPRSAPP
                     ANSDQLIQAIARLDDEFERGEIAEAAYQSQRAALKARALSELRSSDD"
     gene            complement(4604..5047)
                     /locus_tag="VI520_08285"
     CDS             complement(4604..5047)
                     /locus_tag="VI520_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HLF37916.1"
                     /translation="MDLGSVLFGLAMVVLVAAYVLRPIAAGPRGSQGGGQFSSLQAER
                     EHLLDALQELDLDHYMGKVLDKDYRPQRQALTLQGVQVLRRIDELQGSPGPQDSLEAK
                     LEAAVKEQRSLARSETRYCHQCGSRILPADRFCPRCGAVQQEPGV"
     gene            complement(5053..5613)
                     /locus_tag="VI520_08290"
     CDS             complement(5053..5613)
                     /locus_tag="VI520_08290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLF37917.1"
                     /translation="MADDAERVPTQGPPIRRSVTLMVWGVVLAVLLLVGFGQIRAQQG
                     PVGIGSRAPEFTLSTFEGGQVSTDDLKGQVIVVNFWASWCKPCEQEALELEQAYQIYK
                     DQGVAFLGVDYVDIEREALAYLERFEISYPNGPDLGTRISQAFRTQGVPETFVIGPDG
                     RIAAVKIGPYESLEAIVAHIELARGE"
     gene            complement(5625..6101)
                     /locus_tag="VI520_08295"
     CDS             complement(5625..6101)
                     /locus_tag="VI520_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HLF37918.1"
                     /translation="MRQPLAALLLAGFLAASAPPTLVAAQAEQPTDDEVNAIAKGMYC
                     PVCENVPLDVCPTLACKQWRETIREKLTLGWNQEQIEQYFVEQYGDRVLAAPPAHGLN
                     WAVYLIPPAVFLLGAAVLVGAMRSWRRPPVETRVGHDTAAADPYLDRLEEELRARG"
     gene            complement(6105..>7395)
                     /locus_tag="VI520_08300"
     CDS             complement(6105..>7395)
                     /locus_tag="VI520_08300"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HLF37919.1"
                     /translation="GYWGWDPVEIAAFMPFLSGTAFLHSVMVQEKKGMLKHWNVLLII
                     LTYVLVIFGTFLTRSGVLSSVHAFAQSAIGPMFFAFIGITSIVSVRLLLDRWGDLRGE
                     NQLNSLISRESLFLLNNFLFIIILAVCFLGVIFPLASELFTGQTVTVGPEWYERITGP
                     LFAGLLLLMGVAPLSAYGSSSWRLLGRQLWVPGALSLVVLAGLLLGGVRIWAAVLGYW
                     LAGLVALVTLYEFWRGALARHRRHGESVPLALVRLAGRNRRRYGGYIIHLGVVLMALG
                     IIGIELFQTETQGTLARGEQLTLGQHVLVFEGLTQFGTPDGREVSRAEVSVYKDGRFV
                     GQLFPRQDFYVAANQPMTIPGVRSTLEEDLYLLLVGWEPIAQQGATFKVFHNPLVNFV
                     WLGGVVFILGTLVAAWPDRETAPLRRRVQVRAWVSGD"
BASE COUNT         1494 a         2449 c         2253 g         1199 t
ORIGIN      
        1 gcaagtaccc ggagaagtgg gaattggccc agatcaccaa cggagaggga cggaccggag
       61 gaatccgtga ggccctgcag ggggcggacg tagttatcgc gctatcgaag ccgggtccgg
      121 gcgtaatcgt tcctgacgat atccggctga tgggccccga tccgatcgtg tttgcctgtg
      181 ctaatccgat ccctgagatc tggccctggg aagcggcgga ggcgggcgct cgggtggtag
      241 ccaccggccg gtcggatttc cccaatcagg tcaacaactc gcttggcttc cccggtattt
      301 tccgcggcgt actcgatgtc cgcagcacgc gcatcaccga cgaaatgtgt atcgcggcgg
      361 cggaagagct ggcgctcatc gcccaagaga aggggctgga ggccaactcg atcctgccaa
      421 gcatgagcga gtgggaggtc ttcccgcgag ttgccacggc agttggcatg aaggcacagg
      481 aacagggtct cgctcaggta caggtcgcca gagcagatct gtatcagcgc gcccacgacc
      541 ggatccagcg ggcccgagcg atcgtcgcga gcgacatgga gcgggggttc atcaaggagc
      601 caccctcaga ggagacagtc atctacaggc gatagactcg gctcctccct ccaacctggc
      661 ccatccgaaa gcgcgcccgc aatctattcg aggagccgac cattcggaag tggattgacg
      721 agtgcagccg tttgaccaga caatccgcct acgattcggt cgttgtgcgc ttcggggctt
      781 ggacttcctc catgagttcg aggtcccgcc gcaggttgcg gaagcggctg accaggctca
      841 acgcaaacag cgccatcgtc cctaagatca ccccgtaccc gaacagcatg tacccaagcg
      901 tgtccggcgt cgcctcttgt agcatcatcg cgtctctcct cggatcagtc tcaaaacctc
      961 acgcagaacc cacttcaggc cgtcaacatt tccatcttgc gctcttcgac ctgctcggcc
     1021 agccggccca ggcggacccg atgccagagc agcgtgatgt agacaaaggt gaacgtgagc
     1081 aggctgaaga ggaaggtctg cagcattcgg ggggtcatat cgaaggcccc ctcggcggag
     1141 gggtcactgc tgccgattac gaccggatgg atggtgcgga agaagcggat cgacaggaag
     1201 gtgatcggca cgctcacgaa ggccgcgatc ccatagaccg acccgaagcg ggcccgccga
     1261 tctggatcat cgattccttg tcgcaacatc aggtacgcta catacaacaa ttccatgatg
     1321 gtggcggtca ccagtcgcgg gtcccaagtc caccaggtgt tccaggccgg ccgggcccaa
     1381 atcgagccgc tggcgatgtt gatgaaggtg aacacgatcc cgatctctac cgaggccatc
     1441 gccaacaagt cccagtgccg cttccgccgg gcaaggtaga tcgctcccgc cacggcggtc
     1501 agcaagaagg ccaggccgcc cacccagccg gctgccacat ggaagtagaa cacccgctgc
     1561 acctctccca tgattacctc ccgcggcgcg taaccgaaga ccaggtagag ggcgccaacc
     1621 agcagcgcga tggtcactcc ggtcaggact ctgaggttgt taggcattgg ttgcatcgtc
     1681 cgcctccgct tctatcgagc tcatagttca ttcttcgacg acatagtcaa aagtcatgat
     1741 cgcgaccgca acgaacagca ggtcgtagac aatcagcaga ttgaaccaac ctcgaatctc
     1801 cgtcaacgga atcgccagca ggagggaact tgtcgcccgg accgcggcga tcagcaacgg
     1861 cagcacgact ggaaaaagca ggatcggaag caggatgtcg cgggtgcggg cctgcacagc
     1921 catcgccgag aacaaggtgc cgaccgtagt gtacccaatc gacccgagca cgatcactcc
     1981 agccagggcc ggccgaaaca ggttgacgtt gtagagcagg ctgtagatcg gcaggatgat
     2041 cgccgccaca atcaacatga acagcaggtt gccgagggct ttgccgaaga agagcgccga
     2101 acgatcgacc ggggccaaga gcagcccgtc cagacagcct cgctcccgct caaccgccat
     2161 ggcccggttc aggcccaggg tgcctgcgaa ggcgaaggtc acccacagta cccccgccgt
     2221 caccgattgc cgcgctcgca cgtcgagctc gagcgcaaaa ttgaagatca agataaccag
     2281 caacccaaac accagcatgg ccccgagcag ctctcggctg cgcaattcgg cagtcagatc
     2341 tttccaaatc accgctccca ccgcccgcaa gtagctggcg aagaccccgg gggccggtgc
     2401 ccgcagtttg gcttcggcgc gttcaagcat gggtcacctg ccgataggca ttcgccaggt
     2461 cggctgccgg cagttcgccc cggcggaccg agcgggcaat caccccgccg aagagaatgt
     2521 ccacccgtga ggccaacccc gccgcccgca ccagatcgtg ggaggtcatc aacaccgtgc
     2581 ggccctcgct gctcacttgg gtcagcagtc cgtccaaggc ttccgccgcc tcgggatcca
     2641 gcccggtata cggctcatcg aacagcagca gttcggggtc gtgcaagatc ccccgcccga
     2701 tcgccagccg ctgctgcatc cctcgcgaat atttccgaac caagtcgtgg cgacggctgt
     2761 tcagtcctac gatgcccaga acctcttcga tccgggcctc caggtccgcc actccgtaca
     2821 tccggccata gaagcggaga ttctcttggg cggtgagctc gccatagagc aacggttgat
     2881 gcgagacgac tcccaggatc cgacgggcgg cggcggcctg gcctggaagt cgcagtccag
     2941 ccaggcggac ctcgccgaac gtcgggcggg ccaaggaagc caggatccgc agcagggtcg
     3001 tctttccggc tccgtttggc ccgaccactg ctacgaactc gccctgcttg accgcgaggt
     3061 ccagcttgcg cagcaccggc cgcaatccgt aggccttggt caatccgcgg acctcaatca
     3121 tcgctgctcc gcagttcgga caaggcccgc gctttgaggg cggcgcgttg gctctggtag
     3181 gcggcttcag cgatctcccc gcgctcaaac tcgtcgtcga gccgggcgat ggcttgaatg
     3241 agctggtcgg agtttgccgg cggcgcggat cgcggtggct tcccgcgggt ccaccaacgg
     3301 atggtcagca gcccggcacc gaccagtgcg gctgctccga tgagggtgct ctccaaggcc
     3361 ggcgaactgc ctaccgcccc gctgactcgg aagctcagca cctcccccgg gtccagcggt
     3421 ccaccgatgt agttgcgcat cggcagacct cccagatcct ggacgcctcg gtcctccaat
     3481 ccagacactc gagcaccttc cgcgggcacg aggaccgtga ccgcctgcac cggctgctgc
     3541 acctgctgtt cgaagcgggc cgcggccggc aggtcaaaag caaacgccag ctggtcgatt
     3601 cccgagcccg ggggaatcgg ctcacgatcg aggaacccca gctcggtcag ctcgaatcgt
     3661 tctcccagtt cgccttgttc gaaagtcaaa ttgaccgccc caggcggcaa gggaatgttg
     3721 agcggcgaaa cgatgacccg gtcggaaggg ttggccaata cccacaactc caacacccgg
     3781 atcagatcct ccgccgggaa gtcgatgagt acatgcagcc gttccacggc gagcgcgccg
     3841 gcgtcgctgg tggtttcata gatggtcaac ggaagttgga gttggcccgc gtcggccggg
     3901 acgtgagcca gctctgagcg gaagatcacg ccctgatatt ccacggtggt gaaaaacagc
     3961 ctacccggca cggcttcgac ctcgtcgaag cgatatccac cctgccggtc ggctacgacc
     4021 gtctgccgca gcgcttcctg ctctccgtcg aaaccgaata gattgatttg caagcccgcc
     4081 gggacgacgg ccgcggccgt cccggtccga accgtcccgg tcacccttgc cggtgcgctc
     4141 gaatcggcca gcgcgaccgg ctcgagggaa gaactcaaac tcaaggcgta gcccacgacg
     4201 tcccagcgct cttgatcgga aagactgacg aaacctggca tcagtcggtc gatcctgccg
     4261 agggtaacca ccgcatacca gtcttgaggt cgcgccgccc tggcgaaaga aggatccccc
     4321 agcggggcgg gcggaaactc caggttagcc gccaattcgc catctcccag gccccctact
     4381 ccgtggcagg ccgcgcactt atccagatag atggcggctc cggcggtcag gtccggcggg
     4441 ccgtcgggtg gaatgacctc gcctgtggcc actatggttg gaagcggtcg ggccatctgc
     4501 gccgttgcca gggccggcgg gggggtaacg tcgccggcca gcgaacaggc ggcgctcagc
     4561 aggccgagca gggccgcggg gaggaggcgc cccttggcaa acctcaaact cctggctcct
     4621 gctgcaccgc gccacagcgc gggcaaaacc ggtcagccgg gaggattctc gacccacact
     4681 ggtggcagta gcgggtttcg ctccgtgcga gcgacctttg ttccttgacc gccgcctcca
     4741 gcttggcctc cagactgtct tgcgggccgg gggatccctg gagctcgtca atccggcgca
     4801 gcacctggac accctgcagg gtcagcgcct gtcgctgagg acggtaatcc ttgtccagga
     4861 cctttcccat atagtgatcg agatccagtt cttgaagcgc gtccagcaaa tgctcccgtt
     4921 cggcctgaag cgatgagaat tgccccccac cttgcgaacc ccgcggcccg gctgcgatcg
     4981 gccgcagcac ataggccgca accagcacga ccatcgccag cccgaacaag accgacccca
     5041 gatccattgg actcactccc cccgcgcgag ctcaatatga gccacaatcg cctcgagtga
     5101 ttcatatgga ccaatcttga ccgccgcgat ccggccatcc gggccgatca cgaaggtctc
     5161 cgggacaccc tgggtccgaa aggcctgtga aatccgagtg ccgaggtccg ggccgttcgg
     5221 atagctgatt tcgaagcgct ccaaatatgc cagagcctcg cgttcgatat cgacatagtc
     5281 cacgcccagg aacgccacac cttgatcctt atagatctga tacgcctgtt cgagctcaag
     5341 ggcctcctgc tcgcacggct tgcaccacga ggcccagaag ttgaccacga tcacctgccc
     5401 cttcagatcg tctgttgaaa cctggccgcc ctcgaaggtg gacagggtga actcgggtgc
     5461 ccggctgccg atcccgaccg gcccttgctg ggcccggatc tggccgaagc cgaccaagag
     5521 cagtaccgcc agcaccaccc cccataccat gagggtgacc gaacgacgga tcggtggtcc
     5581 ttgggtaggg acgcgctccg cgtcgtcggc cattggctca gcgctcagcc tcgggcccgc
     5641 agctcttctt caagccgatc caggtacgga tctgccgccg ctgtgtcgtg gccgacgcgg
     5701 gtctcaacgg gaggccgcct ccaggagcgc atcgctccga ccaggacggc tgcgcctagg
     5761 aggaagacgg ccggcgggat taggtaaacg gcccaattga ggccatgggc cggaggtgcg
     5821 gcgagaaccc gatcgccata ctgctctacg aaatactgct caatctgctc ttggttccag
     5881 ccaagggtca atttctctcg gatcgtctcc cgccactgct tgcaggccag ggtcgggcag
     5941 acatccagcg gcacgttttc gcagaccggg caatacatcc ccttggcgat ggcattaact
     6001 tcgtcgtcgg tgggttgctc agcttgagcg gcaacgaggg tcggcggcgc actcgccgcc
     6061 aggaagccag ccagcagcag tgcagcgagt ggctgcctca cgccctaatc gcccgacacc
     6121 caggcccgga cctgcacacg gcgccggagc ggtgcggttt cgcggtccgg ccaggctgca
     6181 accagagtcc ccaggataaa cacgacaccg ccgagccaga cgaagttcac cagcgggttg
     6241 tggaacactt tgaacgtggc tccctgctgg gcgatcggtt cccaaccgac caacagcagg
     6301 tagaggtcct cttccaaggt ggaacgcacg ccggggatgg tcatcggctg gttggcggcc
     6361 acgtagaaat cctgtcgggg aaacagctgt ccgacgaacc gcccgtcttt gtagactgag
     6421 acttctgccc gcgaaacttc acgcccgtcg ggcgtgccga actgagtcag cccctcaaat
     6481 accaggacgt gttggccaag ggtgagctgc tcgcctcgag ccagcgtccc ctgggtctcg
     6541 gtctggaaca gctcgatgcc gatgatgccg agcgccatca ggacgacgcc taagtggatg
     6601 atgtagccgc cgtagcgccg ccgattgcgt cccgccaacc gcacgagggc cagcggcacg
     6661 ctctccccat gccgccggtg ccgggccagc gccccccgcc agaattcgta gagggtgacc
     6721 agggcgacca ggccggcgag ccagtagccc aggaccgcgg cccagatccg cactcctccc
     6781 agcagcaggc cagccagcac tacaagggag agcgcgccgg gcacccagag ctggcgtccc
     6841 agcagcctcc aggaggaact tccataggcc gacaggggcg ctacgcccat caagagcagc
     6901 agcccggcga acagtgggcc ggtgattcgc tcgtaccact cgggcccgac cgtcacggtc
     6961 tggccagtga acagttcgga ggccagcggg aagatcactc ccaagaagca aaccgccaga
     7021 atgatgatga acaggaagtt gttgagcagg aagagcgatt cgcgcgagat gagcgagttg
     7081 agctgattct ctccccgcag gtctccccag cgatctagca gcagccggac ggacacaatg
     7141 ctggtgatcc cgatgaaggc aaagaacatc ggcccaatcg ccgattgggc gaaggcatgg
     7201 accgacgaga gcactccaga gcgggtcaga aatgtgccaa agataaccag gacataggtc
     7261 agaatgatca gcagcacgtt ccagtgcttg agcatcccct tcttctcctg caccatcacc
     7321 gagtggagga aggccgttcc gcttagaaag ggcatgaagg ccgcaatttc gaccgggtcc
     7381 caaccccagt aacct
//
LOCUS       DATDPQ010000372         4919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3599209, whole genome shotgun sequence.
ACCESSION   DATDPQ010000372 DATDPQ010000000
VERSION     DATDPQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3599209"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VI520_08305"
     CDS             complement(<1..453)
                     /locus_tag="VI520_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF37920.1"
                     /translation="MADARSIQRKQADPLKADRADESSDAELMRELAKGQEWALEALY
                     DRYASAIMGLALRILGEREPADEVVQETFWRAWSRSASYSAERGAPLSWLFGIAHHLA
                     IDLTRRRASNRPDSGAAKPNPEDRQQVEESVVAALRRKQVLSALADLEP"
     gene            complement(499..951)
                     /locus_tag="VI520_08310"
     CDS             complement(499..951)
                     /locus_tag="VI520_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37921.1"
                     /translation="MRSNTRQRLLAGAALGCAALACNLPFIGGGDEPAPMEVVVGEQP
                     TPAEEPAVSGDSGGGAVASNYPMVGDAYDVAGIEGLLTYSTREEFSAVVQFYEEQMPL
                     AGWTLTEEFTLGPLALLTFSGADSSVQVTVTLEASTKVVSVTILEVEP"
     gene            complement(984..2153)
                     /locus_tag="VI520_08315"
     CDS             complement(984..2153)
                     /locus_tag="VI520_08315"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HLF37922.1"
                     /translation="MAEHGYIVIADITGYTAFLSSSELEHAEDSLRSLINLLIDQTKP
                     PLVISRLQGDAVISYAPQASFLQGQTLVEILENTYVAFRQARERMQLNTTCTCAACQN
                     IPNLDLKFLVHYGSYVLQQTPTYQELVGNDVNLAHRLLKNRITERTGVRAYAGYTEPA
                     VEALGIRDFCIDMSQHTESYEHLGEVLLYIQDLQRVWVRERERRRVLVEPAEAWFTVE
                     RDIPAPPPLVWDYFTKPEYRTVLMGADAMPVENRIQGRTAPGTVYVCAHGDSRIRHAV
                     LDWRPFEYYTYETEMPMGAKSLITMRLSPAAGIEATNVLGICSKPQGNLLARGALGLA
                     RSKIIQEVEEGLVALRKIILEELESGAALRPHRFVVPEEQVAAAVSASVAESLAT"
     gene            complement(2298..3533)
                     /locus_tag="VI520_08320"
     CDS             complement(2298..3533)
                     /locus_tag="VI520_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLF37923.1"
                     /translation="MEKAKDTLGFEFSISAPTEAVFYAFSTGQGWRDWLCDQAIFQPR
                     AGGTYHLGWDTGWYVSGRVEELEKPNRVRLSWRGAEDPAASVVRIELNGRGDHTHGSV
                     EHFGFGEGEAWEDARQKAQRGWEVGLENLESIFSTGADLRITRRPMVGMMGNDFNEKI
                     AAQFGVPVSEGVRLADTVEGMGAAKAGLQSNDVIVELAGKPIRSWPDLGKILQQKRAG
                     ESIPLAFYRGSQRHEVVLQLTARPIPETPLDPVAFARRLREVNREVIEEVREAFRGVS
                     ELEAEFAPTGEWSSKQNLAHLIKDEEENLHRIAQYLNDSEAQYADTWENRWEPLNAIL
                     AVSSTVQELIDRLAKAKETTAALLEASEEPLRARKGVMWRIGMSMLHFPGEHEHEHLE
                     QIKRTLETARVAEPAGQPG"
     gene            3639..3935
                     /gene="gatC"
                     /locus_tag="VI520_08325"
     CDS             3639..3935
                     /gene="gatC"
                     /locus_tag="VI520_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLF37924.1"
                     /translation="MALTAEEVRKIARLARLQLSEEELQRYAQQLSAVLDYAARLGEV
                     DTSAIPPTASVLPLTAPLRPDEVRPSLPLEQVLSNAPSSEAGMFRVPRVLEEQP"
     gene            4025..>4919
                     /locus_tag="VI520_08330"
     CDS             4025..>4919
                     /locus_tag="VI520_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLF37925.1"
                     /translation="MANLTDLTLLEAAAALRAGELSSVELTRACLDRIRALDSAVHAF
                     LAVTDDLALAQAQTADDWLRQNPGRSAPLVHGIPLAVKDVLCVEGAPATAGSRILEGF
                     RPPYTATSVSRLLHAGAVLLGKTNTDEFAMGSSTENSAYGVTHNPWDLDRVPGGSSGG
                     STAAVAARFCYAALGTDTGGSVRQPAAFCGVTGLKTSYGRVSRYGLIAFGSSLDTVGP
                     IARSVADLEPVFHTMSGHDPLDATSMPTPVPKVQLNGNSIQGLPGGLITAGMAIYDTM
                     QFVRPDVSTICIGQAASMAALI"
BASE COUNT          831 a         1684 c         1408 g          996 t
ORIGIN      
        1 cggttccaaa tctgccagcg cgctcaacac ctgtttgcgc cggagtgccg ctacaacgga
       61 ctcctcgacc tgctgcctat cctccggatt cggtttggcc gctcccgaat caggacggtt
      121 ggaggccctt cgccgggtca gatcgatcgc taggtgatga gcgattccaa acaaccagct
      181 cagcggggca cctctctcgg cactgtagct agccgatcgg ctccaggccc gccagaaggt
      241 ctcttggacc acttcatcgg ccggctcccg ttcacccagg atcctcaacg ccaggcccat
      301 gatcgccgaa gcatatcggt catacagcgc ttccagcgcc cactcctggc ctttggccag
      361 ctcgcgcatc agctcggcgt cgctcgattc atcggcccga tccgccttca gtggatcagc
      421 ttgcttcctt tggatactac gagcgtcagc caacggccga attcccaccc atggtactgg
      481 tccggtcccg atacgaggtt atggctccac ttccaagatc gtcaccgaga ccactttggt
      541 gctggcttcc aatgtcaccg taacctggac cgagctgtct gccccggaga aggtcaagag
      601 cgccaggggg ccgagggtga attcctcggt cagggtccag ccggcgagcg gcatctgctc
      661 ctcgtagaac tgcacgaccg ccgagaattc ctcccgggtg ctgtatgtga gcaacccctc
      721 aattccggcg acatcgtacg catcgccgac catcggataa ttgctggcga ccgccccgcc
      781 acctgaatcc ccgctgaccg ccggctcctc cgctggtgtc ggctgctcgc caaccacgac
      841 ctccatcgga gccggctcgt cgccgccgcc tatgaatgga agattgcagg cgagggccgc
      901 acatccgagc gctgcccctg ccagcagtct ctggcgtgtg ttggatctca tcgttttctc
      961 ctgctgggcc tggatgggga gtcttaggtt gcgagtgatt cggccaccga agcgctgacc
     1021 gcagcagcaa cctgttcctc gggcacaacg aaccgatgcg gccgcagggc cgcgccggac
     1081 tcgagctcct cgaggatgat cttgcgaagc gccaccaatc cttcctcaac ttcttgaatt
     1141 attttggatc gcgccaatcc gagcgctccc cgagccaaca agttcccctg gggcttgcta
     1201 cagatgccga gtacgtttgt agcttctatg ccggctgccg gggacaatcg catggttatc
     1261 aacgatttcg cacccattgg catttcggtc tcgtaggtgt aatactcgaa gggtctccag
     1321 tccaggaccg cgtgccgtat tcgggagtct ccgtgggcgc agacatacac cgttccgggc
     1381 gccgtccgcc cctggatgcg gttctcgacc ggcatggcat cggctcccat cagaaccgtg
     1441 cgatattcag gcttggtgaa gtagtcccag accagtggag gcggtgccgg aatatccctc
     1501 tcgaccgtga accaggcctc tgccggctcg acaagaaccc tgcgccgttc gcgctcccgc
     1561 acccagactc gctgcaggtc ctggatgtag agcagcacct cccctaagtg ctcgtagctc
     1621 tccgtgtgct gggacatgtc aatacagaag tccctgattc ccagcgcctc cactgccggt
     1681 tccgtatacc cggcgtaggc cctgacgccg gtccgctcgg taatccggtt cttcagcagc
     1741 cggtgggcca gatttacatc gttgcccacc agttcctgat acgtcggggt ttgctgcaaa
     1801 acatagctgc cgtaatggac caggaatttg aggtccagat tggggatgtt ctggcacgca
     1861 gcgcaggtgc aggtcgtatt gagctgcatg cgctctcggg cctgccgaaa ggcgacgtag
     1921 gtgttttcca ggatctccac cagggtctgc ccctgtagga agctggcttg gggcgcgtag
     1981 ctgatgaccg cgtcgccctg cagccgggag atcaccagcg gcggtttggt ctggtcaatc
     2041 aacagattga tcaggctgcg caggctgtcc tcggcatgct cgagctccga gctgctcaga
     2101 aaagcggtgt agccggtaat gtcggcgatg acaatatagc cgtgctcggc caaatcttcg
     2161 ctcctaattg acctccgagc cgccccggaa gatcacctgc aagtatacgg cacacctccg
     2221 ggtcgagacc tgaaacgcgg cggtggggcc actcggcccc accgcactct cccagattgc
     2281 ccgctttgag tagtcgctta gcccggctga ccggccggtt ctgccaccct cgcggtctcc
     2341 agggttcgct tgatctgttc cagatgctcg tgttcatgct ctccggggaa atgcagcatc
     2401 gacattccaa tccgccacat cacaccctta cgtgcccgga gcggctcttc tgaagcctcc
     2461 aacagcgccg cggtggtctc cttggccttg gccagccgat cgatcagctc ctgtaccgtc
     2521 gaactgacgg caaggatggc gttaagcggc tcccagcggt tctcccaggt gtccgcgtat
     2581 tgggcttcgc tgtcgttcaa gtattgcgcg atccgatgta ggttctcctc ctcatccttg
     2641 atcagatgag ccagattctg tttggaggac cactccccgg tcggcgcgaa ctcggcttcc
     2701 aactcgctga cgccccgaaa cgcctctcgg acctcttcga tgacctcccg gttgacctcg
     2761 cgtagcctcc gggcgaaggc caccggatcc agcggcgtct cggggatggg ccgggccgtg
     2821 agctgtagta caacctcatg tcgctggctg ccgcggtaga acgccagcgg aatgctctcg
     2881 ccggcccgct tctgctgcag gatcttccca agatctggcc agctccggat tggcttgccg
     2941 gcgagctcga caattacatc gttcgactgc aacccggctt tggcggctcc catcccctcc
     3001 accgtatcgg cgagccgcac tccttccgag accggcaccc cgaactgggc cgcaatcttt
     3061 tcattgaagt cgttgcccat catccccacc attgggcggc gggtgatccg caggtccgct
     3121 ccggtggaaa agatcgactc gagattctcc agcccgactt cccagcctcg ctgcgccttc
     3181 tggcgggcgt cctcccaggc ctccccctcg ccaaaaccaa agtgctcgac cgaaccatgg
     3241 gtgtgatcgc cacggccatt cagctcgatc cggactacgc tggcggccgg atcctcggct
     3301 cctctccagg acagccgcac ccggttaggc ttttccagtt cttcgactcg gccggaaacg
     3361 taccagccgg tatcccagcc caggtgatac gtgcctccgg cccgcggctg gaagatggcc
     3421 tgatcacaca gccagtctcg ccatccttgc ccagtgctga atgcgtaaaa caccgcttcg
     3481 gttggggccg aaatcgagaa ctcgaagccg agggtgtcct tcgctttttc catcttattc
     3541 tccgcgctcg ttggggtccc tgattctacc cagcccggat cgcggaagtt catcccaccc
     3601 gcgccaagcc caggtcgcca cgctataata ccggcaaagt ggctctcacc gcagaggaag
     3661 tccggaaaat cgcgcggctt gcccgcctcc agttgagcga ggaagagctc cagcgctacg
     3721 cccagcaact gtcagccgtc ttggactacg ccgcccgcct gggggaggtc gacacttccg
     3781 cgatcccgcc cactgcatcg gtgcttccgc ttactgctcc gctccgcccc gatgaggtcc
     3841 gtccaagcct tcccctcgag caggtgctgt ccaatgcgcc ttcctccgag gcgggcatgt
     3901 tccgggtccc ccgggtcctg gaagagcagc cttaggcgcg ggcgaggcgt cgaatcgcgg
     3961 cgctcggcag tcaccctccg ggtcaccacc cccggaatga ccggcccacc cggaccatcc
     4021 tgagatggca aacctcaccg atctgacgct gctagaagcc gccgctgcct tgcgcgccgg
     4081 cgagctctca tccgtcgagc tgactcgggc ttgtttggac cgcattcgag ccctcgattc
     4141 ggcggtccac gcttttcttg ccgtaacgga tgatctggcc ctggctcaag ctcagaccgc
     4201 cgatgactgg ctgcgccaga atccgggccg ctcggccccg ctcgtgcacg ggattccgct
     4261 cgcggtgaaa gatgtgctgt gtgtcgaggg agcgccggct actgccggtt ccagaatcct
     4321 ggagggattc cgcccaccct acaccgccac cagcgtctcc cgcctgttgc atgccggggc
     4381 agtcctgctt ggcaagacca acaccgacga atttgcgatg ggttcctcga ccgagaattc
     4441 ggcctacggg gtcacccaca acccatggga tttggatcgg gtaccgggtg gttcttccgg
     4501 aggctcgacc gccgcggtgg ccgcccggtt ctgttacgcc gccctcggca ccgacaccgg
     4561 tggctccgtc cggcagccgg ccgcgttctg cggcgtcacc ggtttgaaga cttcctacgg
     4621 ccgggtctct cgctacggtc tgatcgcgtt cgggtcctcg ttggacacag tggggccaat
     4681 cgctcgcagc gtggccgacc tggagccggt tttccacacc atgtccggtc acgaccccct
     4741 ggacgcgacc tccatgccga ctccggtccc taaggtccag ctgaacggaa atagcatcca
     4801 aggattgccg ggcggtttga ttaccgccgg gatggcgatc tatgacacga tgcagtttgt
     4861 ccgccccgat gtctccacca tctgcatcgg acaggcggcc agcatggcgg cgctgatcc
//
LOCUS       DATDPQ010000373         7657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3378648, whole genome shotgun sequence.
ACCESSION   DATDPQ010000373 DATDPQ010000000
VERSION     DATDPQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378648"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VI520_08335"
     CDS             <1..324
                     /locus_tag="VI520_08335"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HLF37926.1"
                     /translation="LTIEKLMELMPSAFLPEKAAGVTAEVQFHLKGAGGGDWILGIRD
                     GSCTVRPGVMEAARLTLTAEAADYLAIVTGKLDAMSGFAQGKIKLKGDLSLAMKLMGF
                     FKIPE"
     gene            355..427
                     /locus_tag="VI520_08340"
     tRNA            355..427
                     /locus_tag="VI520_08340"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:389..391,aa:Glu,seq:ctc)
     gene            580..810
                     /locus_tag="VI520_08345"
     CDS             580..810
                     /locus_tag="VI520_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HLF37927.1"
                     /translation="MPPLDPTILANELHDGVIQELSALLLQMETYERRLQKDPEAASQ
                     DLKRMKDQTRSTLNQLRQLVTRLRDGGPPSPR"
     gene            807..1463
                     /locus_tag="VI520_08350"
     CDS             807..1463
                     /locus_tag="VI520_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLF37928.1"
                     /translation="MTDRTYRIIIADDHLVVREAIRVMLEMEPEFEVVGEASDGKRAL
                     ELAAQLEPDLVLMDIRMEGMDGVEATRLLRQSHPKMGVLILTGFGDDEVLLNAVEAGA
                     QGFLLKDASADEVKSAILRVVQGESHMTPSLLRKLLDELAEREQRPEPAHSELTPREK
                     EVLLALSRGLSNDEIAKELVISEKTVKTHLGSIFGKLQVGGRAQAMLYAIREGLVELH
                     "
     gene            1917..>7657
                     /locus_tag="VI520_08355"
     CDS             1917..>7657
                     /locus_tag="VI520_08355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLF37929.1"
                     /translation="MRSTIISSRAHGTDWSWFNSTALALMPVLLVEPPSASKLLAFVA
                     LFFGAMFFLYALKYYASMALILIANGNSNGNGNGNGHKNGNGHKNGNGNGHGLKGLSR
                     NGGDKNKLPYHPFISIHLPMYNETAVVDRLLQSCTSLAYDNYEVVVADDSTDETSRIL
                     DQRWASHSRVKISRRKSRAGFKGGALQKALQVTDPRAEFIAVFDADFIPPPDILQQFL
                     SYFYGINGNGNGHSETERPTLIDDRLAVVQGYQWHILNASENWITRGIRTEFAGSYVI
                     ERSSQQLTGAMKMISGSVFMVRADVMRQHGWGTSITEDWELTLRLYLSGYKVLYTPFI
                     QAPSECVATFKQLARQRMRWAEGHTFNVKKYFFQVMSSPNISLPEKLEFIYFAPYYLQ
                     SIFFILGTVCWLLSEMVFRYRLPMLPATLGWTLVFTNAISLVLMNLTGLFMERGVRRN
                     WTGLLSFVLLTFMLVPYQAYAALKGLIEPHEGGWHRTQKTGVITDIVDKLGLGKRMRR
                     LLPKQKNTKAGGIDLARRLGLPAAKIAARLPEPIRRRARGISLGVRVASGLAGLLVLL
                     AILAGGISVVSAAPDAFYLHTPPLNDGESMNTATGAGVASMVFDTPGQTARWYSSLNY
                     PGGGGDSTLDAGAYAMNLYFDALPGGGWWDANYSLRKQLTISTGAVGVSTGHEIAITL
                     DHAALVAAGKSLSSGDDVRLAYFNGSSWVQLDRVRDPLTSWNSASTKLWFALAGAILP
                     SSSDANYYLYYSNPAAGAPPADLNAVFNLADDFEDGILSVQFLVSTSGAATVNEAFGG
                     AIIDGGWDDGDAAIIIPESALPFNRRFAIRTITKLESGDSGCCAPSETKLLGVVESVF
                     DPQVTSSATEDARRRIMVTHQANTGDVFIFYLDPTDTPHYWTGAVWTTTPTSWTNWTL
                     NTYHVVELISGGASWRIVARDMGGSVLTQTTPVAWTAVQDTGDDFWSYWGEVYTDQHW
                     AFQTTDWLDIRDYVTPEPSLVAGSEEGIPSVAVTVRVHHTRPDGTDPQLIVTNSTTIT
                     PGTADPLVLSLGSAAAQNFTSADPRKLRVEIQVNSVTNGGSFTLAYDSAADQSRLETP
                     SITVLDVALILAIAGVFIPIITALATERRRMATRLISVILALFLALAVLASQVIPVTA
                     NPDSFYLHDTSPGVMFEEVEPGGDGKDYTPPDGDVAVTNWTDFGGNSVHFDELDETTT
                     DDDTTYTQTSSASGGTGDDLQVSLADLPDPLTNVYHVVEYRWRNAGNSTGDAAIAFSA
                     ELLQGNTVIASQSQSAATVTGQLAYTTSTFTLSGAQADSITDYNDLRIRFNVTTKSAG
                     SSGSRILRVSWAALTVNWPLSVSTSASLTAATSHLYLAAISTKNASSVVQSVSGLGLT
                     WSFVDRQCGGRNQTMVEVWKAIGTPTGDGVVTASFDVAEHNAVISVSRYSGVNPASPI
                     GGVTSANSVGVNGACSGGTDALSGSVNLTTTAANSYAYGAFALRSRTMTAGSGYQERD
                     EIHFGVASGEMAGVGTEDKLVATASTVAVDATFNVTLDWAVVAVEIKAAGTSPAGKTM
                     DGNVGTGAATLSFNTSGQNAYWYTGRSYPTGSGDASIAAGDYTLNMYLNALPAAGFPQ
                     VATTNTSVDNTNDTSNTVNLPSGIQSGDLLIVIFGFKVDTNVITWPNGWTSFFRATQG
                     PNAVVEAAWRKADGSEGSTIAVNTSANSRTSHNSYRITGAEDPAIQPPQSATATGNSA
                     TPNPPSLTPTGGAKDYLWIAVETNNHASAANTITAYPSSYTGGIKADGDTTNSTTGSA
                     TRQLNAASEDPGTFTIQSAVAWAAGTLVVHPASNPSVDVQVTVSHTALDGSGATTIVQ
                     SSTTTINSGTTDPLAISIGSGALQTFTAGNPRLLRVQVQVTGVSGSGFVLDYDGTCAT
                     SRCSSLDTPAVTVP"
BASE COUNT         1504 a         2492 c         2276 g         1385 t
ORIGIN      
        1 ctcacgatcg agaaactgat ggagttgatg cccagcgcgt tcttgcccga gaaggccgcc
       61 ggagtgaccg ccgaggtcca gtttcatttg aagggtgcag gaggtggaga ctggattctg
      121 ggaattcggg acggtagctg caccgttcga cccggggtga tggaagccgc ccgcctcacc
      181 ctcacggccg aggccgccga ctacctggca atcgtgaccg gaaagctgga cgcgatgtcc
      241 ggcttcgccc aagggaagat caagttgaag ggggatctat cgctggcgat gaagttgatg
      301 gggttcttca agatccctga gtgaggttcg gcggtgatac aatccgcccg gttcgccccc
      361 atcgtctagg ggcccaggac gcggccctct caaggccgaa accggggttc gaatccccgt
      421 gggggcactc cgccgcattc gcatacgctt cccgtcctaa gtcaggaagt tctgcttaat
      481 cagggagtgc gcctaccggg caggcagtcc tagcgtcgca ttgcggccgg tttcggtttg
      541 acggtatact cggccgcttc tacccaccta gccacgatca tgccacctct tgacccgacc
      601 attctggcga acgagctcca cgacggagtg atccaggagc tttccgccct cttgctgcag
      661 atggaaacct atgaaagacg cctgcagaaa gaccccgaag ccgcaagcca ggacctgaag
      721 cgaatgaagg accaaactcg ctccaccttg aaccagctgc ggcagctggt gacccggctc
      781 cgtgacggcg gaccgccatc cccccgatga ccgacaggac ttaccgcatc attattgccg
      841 acgatcatct ggtcgtccgc gaggcaatcc gggtcatgtt ggaaatggag ccggagttcg
      901 aggtcgtcgg agaagcatct gacggcaagc gggcattgga gctggcggcc cagctcgagc
      961 ccgatctggt gttgatggac atccggatgg aggggatgga cggggtcgag gccacccgcc
     1021 tgcttcgcca aagtcacccg aaaatgggcg tcctcattct gaccgggttc ggcgatgacg
     1081 aagtgctgct caatgccgtg gaagccggag cccaaggatt cctgctgaaa gacgcgagcg
     1141 cagatgaggt caagagtgca attctgcggg tcgtgcaggg ggagtcgcac atgactcctt
     1201 cactcctgcg caagttgctc gatgagctgg ctgagcgcga gcagcggccg gagccggctc
     1261 actccgagtt gaccccccgc gaaaaagagg tcctgctcgc attgtcgcgt ggactgtcaa
     1321 atgacgagat cgccaaggag ttggtgatct cagaaaagac tgtcaagact catctgggca
     1381 gcatcttcgg caagctccag gtggggggcc gggcccaagc gatgctttat gccatccggg
     1441 aagggttggt agagctgcac taagtcctag gaccaacgcc cgatccgacc agcccgattc
     1501 tcagaccaaa ggccgaacac agttcggcct ttgtgcttcc caggttcagt tcggcctgtg
     1561 gatgccggga accattgctc gtggaataat ctacatacgt ttcgagtgga aacgccttca
     1621 gacggacatc cgtctgttcg gcgttttttt attccaggtg cagatgagcg cttgatcggg
     1681 aagggaaggc agatggcaga cgcgggaact ctctccaatt gtaagttgac tgaagactcg
     1741 ttcgactggg ccaagttcta tgatgacctg gatatacgtg taatcggaac tctctcgaaa
     1801 ggtaggcacg gacggctctc gctggccgtt ctttcttcca gcggaagccg cctgtatgcg
     1861 ttcatgcgcg aatccgatct tggacgtctg gaggactgta cccgactaga cgacgcatga
     1921 ggtcaacaat catctcttcc cgggcgcacg gcacggactg gtcctggttt aactccaccg
     1981 cgctggcgct catgcccgta ttgctggttg aaccgccatc agcctcgaag ctgttggcat
     2041 tcgtcgcgct gttcttcggg gcgatgtttt tcctctacgc cctcaagtat tacgccagca
     2101 tggcgctgat cctgatcgca aacgggaaca gcaacggcaa cgggaatggc aatggccaca
     2161 agaacgggaa tggccacaag aatggcaacg ggaacggcca cggcctgaag ggcctgtccc
     2221 gcaacggcgg cgacaagaac aagctgcctt accacccctt catttccatc cacctgccga
     2281 tgtacaacga aacggcagtc gtcgatcgcc tgctccaatc ctgcacctcg ctcgcctacg
     2341 acaactacga agtggtggtc gccgatgact cgaccgacga gacctcccgc atcctggacc
     2401 aacgctgggc cagccatagc cgggtgaaga tctcccgccg caagagcagg gccggcttca
     2461 agggtggggc gctgcagaag gccctccagg tcaccgatcc gcgggccgag ttcatcgcgg
     2521 tgttcgatgc cgacttcatc ccacccccgg acatcctgca gcaattcctg tcctacttct
     2581 acggcattaa tggcaacggc aacggccact cggaaaccga gcggccgacg ctgatcgatg
     2641 atcggctggc ggtggtccag ggctaccagt ggcacattct gaacgcttca gaaaactgga
     2701 ttacccgggg catccggacc gagtttgccg gctcctacgt gattgagcgc tccagccagc
     2761 aactgaccgg cgcgatgaaa atgatttccg gttcggtctt catggtccgg gccgacgtca
     2821 tgcgccagca tggctggggg acttcgatca ccgaggactg ggagctcacc ctgcggctct
     2881 atctcagcgg ctacaaggtc ctctacacgc cgttcattca agccccctcg gaatgcgtgg
     2941 cgactttcaa gcagctggct cgccagcgca tgcggtgggc cgaagggcac accttcaacg
     3001 tcaagaagta cttcttccag gtcatgtcca gccccaacat cagcctcccc gagaagctcg
     3061 agttcattta cttcgccccg tactacctgc aatccatctt cttcatcctg ggtaccgtct
     3121 gctggctgct ttccgaaatg gttttccgct accggctgcc gatgctgccg gccaccttgg
     3181 gttggacgct ggtcttcacc aatgccatct cgctggtcct gatgaatctg accggcctgt
     3241 tcatggagcg cggcgtccgg cgcaactgga ccgggttgct ctcgttcgtt ctgctcacct
     3301 tcatgctggt gccttatcag gcctacgccg cgttgaaggg tctaatcgag ccgcacgagg
     3361 gcggctggca ccgaacccag aagaccgggg tcatcaccga catcgtggac aagctgggcc
     3421 tgggcaagcg aatgcgccga ctgctgccca agcagaagaa taccaaggct ggcgggattg
     3481 atctggcccg gcggctcggg ctgccggcgg ccaagatcgc ggcccggctg ccggagccca
     3541 tccgccggcg ggcgcgcggg ataagcctgg gcgtccgggt cgcgtcaggt ctggcggggc
     3601 tgctggtctt gctggccatc ctggccggcg ggatctcggt ggtctccgcc gcgccggatg
     3661 ccttctacct gcacacaccg cccctcaacg acggcgagtc catgaacacc gccaccggtg
     3721 cgggggtggc ctccatggtg ttcgacacac ccgggcagac tgcccgctgg tattcgagtc
     3781 tcaactaccc tggcggggga ggcgactcca ccctggacgc gggcgcctat gcgatgaatc
     3841 tgtacttcga tgccctccct gggggcggct ggtgggatgc caactactcc cttcgcaagc
     3901 agctcaccat ctcgaccggc gcggtaggag tctccaccgg gcacgagatc gccataacct
     3961 tagaccacgc cgccctggtg gctgccggca aatcgctgag ttcaggggat gacgttcggc
     4021 tcgcctactt caacgggtct tcctgggtgc agctcgatcg ggtgcgcgat ccgctcacca
     4081 gctggaactc ggcgagtacc aaactgtggt tcgcactggc gggcgcgatc cttcccagca
     4141 gcagcgacgc caactactac ctgtactaca gtaatccggc ggcaggggcc ccgcccgctg
     4201 acctgaacgc ggtcttcaac ctggcggatg attttgaaga cgggatcctt tcggtccaat
     4261 tcctcgtttc caccagcggc gccgccaccg tcaacgaggc ctttggcggg gctatcatcg
     4321 atggcgggtg ggacgacggg gatgcagcca tcatcatccc cgaaagtgct ctgcccttca
     4381 accgccgctt cgcgatccga accatcacca aactggaaag tggagactcc gggtgctgcg
     4441 cgccctccga gactaagttg cttggcgtgg tcgaaagcgt atttgacccg caggtgacct
     4501 cgagtgcgac cgaagatgca cgccgccgga tcatggtcac ccaccaggcg aacactggcg
     4561 acgtcttcat cttctacctc gacccaaccg acacaccgca ttattggacc ggcgcggtct
     4621 ggacgacaac tccgaccagc tggaccaact ggactctcaa cacctaccat gtggtcgagc
     4681 tgatcagtgg cggcgccagc tggcggatcg tggcccgcga catgggtggg tcggtgctga
     4741 ctcagacaac tccggtcgcc tggaccgcgg tccaggacac tggagacgac ttctggtcat
     4801 actggggaga ggtctacacc gatcagcact gggccttcca gaccacggat tggctcgata
     4861 tccgggacta tgtaaccccg gagcccagcc tcgtggccgg atcggaagaa gggattcctt
     4921 cggtagccgt gactgtgcgg gtccaccaca ctcggccaga cggcaccgat ccgcagctga
     4981 ttgtgaccaa ttcgacgacg atcacccctg gcaccgccga tccgctggtg ctttcgttgg
     5041 gcagcgccgc ggcccagaac ttcacctcgg ccgatccgcg caagcttcga gtagagatcc
     5101 aggtcaacag cgtcaccaac ggcggcagct tcacgctggc ttacgactcg gccgccgacc
     5161 agtctcgcct ggagaccccc agcatcaccg tgctcgacgt cgccttgatc ctggcgatcg
     5221 ccggggtctt cattccaatc attaccgccc tggccaccga acggcgccgc atggcgaccc
     5281 ggctaatcag tgtgatcctt gccctcttcc tggcgctggc ggtgcttgcc tcgcaggtca
     5341 ttccggtcac cgccaatccg gattcgttct acctgcacga cacgagcccc ggcgtcatgt
     5401 tcgaagaggt cgagcccggc ggggacggaa aggactacac cccgcccgac ggggatgtcg
     5461 ccgtaacgaa ctggaccgac ttcggtggca acagcgtcca cttcgatgag ctcgatgaga
     5521 ccaccaccga cgatgacacg acttacaccc agaccagctc ggccagcggg ggcaccggcg
     5581 atgacctgca agtcagcctg gcggatctcc ccgacccact gaccaacgtc taccatgtcg
     5641 tcgagtaccg gtggcgcaac gctgggaaca gcaccggaga cgcggccatc gctttcagtg
     5701 cggaactgct gcaagggaac accgtcattg cttcccaatc gcagtccgcc gcgacggtga
     5761 caggtcagct cgcctacacc accagcacct tcacgctttc gggggcccag gccgacagca
     5821 tcaccgacta caacgacctg cgcatccgct tcaatgtcac caccaagagc gcaggtagct
     5881 ctggttcgcg catcctgcgg gtcagctggg ccgcgcttac ggtcaactgg ccgctctcgg
     5941 tatccacctc cgccagcctg actgcggcca cgagccacct gtaccttgcc gcaatctcca
     6001 ccaagaacgc tagctcggtg gtgcagtcgg tgagcggcct ggggctcacc tggagcttcg
     6061 tggaccgcca gtgcggtggg cgcaaccaga ccatggtgga ggtctggaaa gcgattggca
     6121 ctccaaccgg agacggcgtc gtgacggcct catttgatgt ggctgaacac aacgcggtca
     6181 tctccgtctc ccgatattca ggggtgaatc cggctagccc gatcggggga gtgacctctg
     6241 ccaacagcgt cggcgtgaac ggggcatgca gtggcggaac ggatgcgctg agcggatcgg
     6301 taaacctcac caccaccgcg gcgaattcat acgcctacgg ggcgttcgcc ctgcgcagcc
     6361 ggaccatgac cgccgggagc ggttaccagg agcgggacga gatccacttc ggcgtcgcca
     6421 gcggggagat ggccggagtg gggaccgaag acaagctggt ggcgactgcc tccaccgtgg
     6481 ccgtcgatgc gaccttcaac gtcaccctcg attgggcggt cgtggcggta gagatcaaag
     6541 cggcaggcac gagccccgcg ggcaagacga tggacggcaa cgtcggcacc ggcgcggcca
     6601 ccctgagctt caatacttca ggccagaacg cctactggta taccggccgc agctacccga
     6661 ccgggtcagg cgacgcctcg attgcagccg gggactacac cctcaacatg tacctcaacg
     6721 cgctccccgc cgccggcttt ccccaggtcg ccaccaccaa tacctcggtc gataacacca
     6781 acgacacgtc aaacacggtg aacctgccct ccggcatcca gtccggcgat ctactcatcg
     6841 tcatcttcgg gttcaaggtc gacaccaacg taatcacctg gccgaacggc tggacctcat
     6901 tcttccgcgc aacccaaggg cccaacgccg tcgtcgaagc ggcatggagg aaggccgatg
     6961 gaagcgaagg gtcgaccatc gcggttaaca cctcggctaa ctcaaggacc tcgcacaaca
     7021 gctaccggat caccggagcg gaagatccgg cgatccagcc ccctcagagc gccacagcga
     7081 ccggcaatag tgccactccg aacccgccga gcctgacccc cacaggcgga gccaaagact
     7141 acctctggat cgcggtcgag accaataacc acgccagtgc cgcgaacacg atcactgcct
     7201 atccgagcag ctacacgggc ggcatcaagg ccgacggcga caccaccaac tcgaccaccg
     7261 gctcggccac tcgccaactc aatgcagcct cggaggaccc aggaaccttc acaattcagt
     7321 cagccgtcgc ctgggccgcc ggaacgctcg tggtgcatcc ggcgtccaac ccctctgtgg
     7381 atgtccaggt aacggtctcc catacggccc tggatggttc tggcgccaca accattgtcc
     7441 agtcctcaac caccaccatc aattccggca cgaccgatcc gttggcgatc tcgatcggct
     7501 ccggcgcgtt gcagaccttt accgcaggga atccacggtt gcttcgggtt caggtccagg
     7561 ttacaggagt gagcggctcg ggctttgtgc tggattatga cggtacctgc gcgaccagcc
     7621 gctgcagcag cctggacacg cccgccgtga cggtccc
//
LOCUS       DATDPQ010000374         5732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3911703, whole genome shotgun sequence.
ACCESSION   DATDPQ010000374 DATDPQ010000000
VERSION     DATDPQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911703"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VI520_08360"
     CDS             complement(<1..1127)
                     /locus_tag="VI520_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLF37930.1"
                     /translation="MTIKSAGPETAIRNYPKLLARYQQLLELTSDLAATFDLDTLLQR
                     IVVAARELTESEASSLLLYDHPSHSLYFEAATGALMGGVGQRTIPADNSIAGWVFTHG
                     EPIVSDDVLNDPRFYREMDVLTRFETHSILGVPLRTKDKILGVIEAVNKHEGFFSDED
                     VQILETLAAQAAIALENSRLFNQSDLIAEIVHELRTPLGALTAAAHLLQRAELPPEQH
                     QKLSETILTEVRRLNDVSTDFLELSRLESGRVHFQREPVHLGGLVQESLEVVRAQAQT
                     RGISLESEIDASVAPVYGDRKLLKQLLLNLLTNAIKYNSHSGKVVVALRSEEGEVLMQ
                     VRDTGRGISPENQTRLFEKFYRVSDSDDEVQGTGLGLAIAKR"
     gene            complement(1157..2302)
                     /locus_tag="VI520_08365"
     CDS             complement(1157..2302)
                     /locus_tag="VI520_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLF37931.1"
                     /translation="MAEKILIIDDDIDTLKLVGLMLERQGYQIAVASNGTIGLSKASS
                     ERPDLILLDVMMPDMDGYQVTERLRSDPSLSHIPIVMFTAKTMVDDKVKGFESGVDDY
                     LTKPTHPAELTAHVKAVLSRSAGTPTKEAPEQAQIVSFFGTRAGLGTSTLALNVGVQM
                     QQNGLDVIIAEFNPGQGSIALDLNISAPTGLTTLLSRSLKDIHLRSVESEIVTHPSGV
                     RLLLASYQPQESELDQAVAQMEAVVKNLAAMCTVLVLDLGAGLRPYSKPLLLASDRVI
                     VVVEPVYPSNVIARAVLDDLFSSGLQRGRTSMAMITRERTSLNIPWKQVEADLGIEMA
                     GIISPAPELAQQASQSGTPLVILHRDTLTGDQIRKMSETISTKLALS"
     gene            2488..5709
                     /locus_tag="VI520_08370"
     CDS             2488..5709
                     /locus_tag="VI520_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLF37932.1"
                     /translation="MTIAGWGLESSFYLGLPTREAGYLLLGLYLLVVALTVRKPAIRL
                     PRKLGWLLFASAGLLLGAFVLSGAITIRILTAAAAPEQLQVGAGSTFPLLGATPWMIA
                     GGFFGDLPAVLLGLLGGLVRGGWGTQRLLTPFGIAAQAALFSWLIRRPYSEWPGRLAR
                     RPLPSALATGLFFGFLRSLEDFVYLPGDFLGALDQTTATLGPTVLASVLELGLAGILT
                     AGLKAAFASHWQAPRHLILGPYNRTLAARLVTVMVSLGLAGGAALTYGNWLLSRASAQ
                     RIISDQMLQTASQVAEGIPFFIQTGRTEIRSIAESLPEQGAAREISAAALTEAMADRA
                     YFTRLAVLDSGGAAWAAWQGSGSALPREIEVAAQTGAPQELVVGGELPGEGVQLVFLT
                     PLASSSEGPGMLVGWTDLDHNPILEPLNARLQAYTAGEALVVDNRGRVILHPDPGQWL
                     RPIIYPDPASAAFFHASDSTGSRSWVYSLQVEGYPWRVIVSTPQSALDALALSTTVNL
                     FLLLVSLGGVLIAAVHLMSRRLTRPLRDMAGTAEAIALGDLDRQVPAGGEDEIGRLAG
                     SFERMRQALKARLGELNLLLATSRRMAESFDLAEALPIVLNRVRDLMNVDCVRLVLSG
                     SEAELETYQAGILGKGWESLDLGILELTRKRGQFSLENPARAGAVLDLGGISDGLEAL
                     IAFPVKHEADDLGALWLGHGHPRAFTKSEVRLLSIFAGQLAISLENVGLYRRAERERS
                     RLLAILSSTPDAVIVTDARGRVSLANPAADAVLTTRAEDAIGRPIGELVRVPELAALF
                     TEPLPPSRSLEIKIDGGRVLYASASDVPGGQLGRVCVLSDITHYKVLDALKSEFVSTV
                     SHDLRSPLTLMRGYSSMVANVGSVTSQQQELLDKIQTSVDRMTKLVDDLLDLGRIEGG
                     IGLSLEAVEISAVLQEVLTSFRPLALNKQVALEVQLGSSLLPVQADPILLRQAIANLV
                     DNAIKYSQPGARVSLRADQVEGRQIVQVQDSGVGVASADQPRLFEKFFRVGEGKGAGL
                     GLAIVKSIVEQHQGRVSVESRLGRGSTFTIAFPMRVERVRAAA"
BASE COUNT         1003 a         1724 c         1845 g         1160 t
ORIGIN      
        1 cgcttggcaa tcgccagtcc gagaccggtt ccctgcacct catcgtcgct atccgagacc
       61 cggtagaact tctcgaacaa tcgggtctgg ttttccggcg aaatgcccct cccggtgtct
      121 cgtacttgca tcaagacttc gccttcctca gagcgcaagg cgacgacgac tttgccactg
      181 tgggagttgt acttgatggc attggtcagc aggttgagca ggagctgctt gagcagtttc
      241 cggtcgccgt aaactggggc cactgaggcg tcgatttcgc tctcgagcga gattcccctg
      301 gtttgggcct gggctcggac cacctccagc gactcttgga ccagcccacc caggtgcacc
      361 ggctcgcgct ggaagtgaac ccgaccagac tccagccgag agagttccag aaagtcggtc
      421 gacacatcgt tgagccgcct gacctcggtg aggatcgtct cgctgagctt ctgatgctgc
      481 tccggcggca gctctgcccg ctgcagcaaa tgggctgcgg cggtcaacgc ccccaggggg
      541 gtgcgcagct cgtgcacgat ctcggcaatc aggtcgcttt gattaaacag ccggctgttc
      601 tcgagcgcga tcgctgcctg ggcggcgagc gtttctagga tctgcacgtc ctcgtcgctg
      661 aagaaacctt cgtgcttgtt aaccgcttcg atgaccccca aaatcttgtc cttggtccgc
      721 agcggaacgc cgaggatact gtgggtctcg aaacgggtca gcacgtccat ctcccggtaa
      781 aaccgcgggt cgttcaacac gtcgtcggaa acgatcggct cgccatgggt aaagacccag
      841 ccggcgatgc tgttgtcggc cggaatcgtc cgctgaccaa ccccgcccat cagggcaccg
      901 gtggcggcct cgaaataaag cgagtggctg gggtgatcgt acagcagcag ggaggaggcc
      961 tcgctttcgg tcagctcgcg ggccgcgacg acgatccgct gcagcagggt gtccaggtcg
     1021 aaggtggcgg ccagatcgga cgtcagctcg agcagctgtt ggtagcgtgc aagcagctta
     1081 ggataattcc ggatcgcggt ctctggaccg gcggacttaa tcgtcacaag tcaccatcag
     1141 cggcgggaca actgagctag ctgagagcca gcttggtaga aatcgtctcg gacatcttac
     1201 ggatctgatc cccggtgagg gtgtcccggt gcaatatgac caaaggcgtg ccgctttggg
     1261 aggcctgctg ggcgagttcc ggtgcgggag agatgatccc ggccatctcg atccccaggt
     1321 ccgcctccac ctgtttccag ggaatgttga gactggtacg ctcgcgggtg atcatcgcca
     1381 tactggtgcg gccgcgctgc aggccgctcg agaacaggtc atccagtacc gctcgggcga
     1441 tgacgttgga aggatagacc ggctccacga caacgatcac ccgatcgctc gccaacagca
     1501 gtggcttcga gtagggtcgc agtccggcac ccaggtccag caccaggacg gtgcacatgg
     1561 cggccaggtt cttgaccacc gcctccatct gggcaaccgc ctgatccaac tcggattcct
     1621 ggggttggta ggaggcaagc agcaagcgga cgccactggg gtgggtgacg atttccgatt
     1681 ctaccgaacg aaggtggatg tctttgagag agcgggagag cagggtcgtc aggccggtgg
     1741 gggcactgat attcaaatcc agggcgattg aaccctggcc ggggttgaac tcggcaatga
     1801 tgacatctag gccgttctgt tgcatctgca ccccgacgtt gagagccagg gtcgaggtcc
     1861 cgagcccggc ccgggtcccg aagaagctga cgatctgggc ctgttcagga gcttccttgg
     1921 tcggagtacc ggccgatcga gagagcacgg ccttgacgtg ggcagtcagc tcagccgggt
     1981 gggtcggctt ggtcagatag tcatccacgc cggactcaaa ccctttcacc ttgtcatcca
     2041 ccatggtctt ggcggtgaac atgacgatcg ggatatgaga cagcgatgga tcggagcgca
     2101 agcgctccgt aacctgatat ccgtccatgt ccggcatcat gacgtccaac aagatcaggt
     2161 cggggcgttc cgaggaagcc ttggaaagtc ctatagtccc gttgctggcg accgcgatct
     2221 gataaccctg ccgctcgagc atcaggccga cgagcttcag cgtatcgata tcgtcatcga
     2281 tgatgaggat cttttcggcc attaagctcg atttggcgag agcgagggca gtctagcata
     2341 aagccctatg ccgggcaagg attcgtactt cccacacctt atgtcagcat ggccggcgct
     2401 tgagaacgtc ggctggtcgg tcgcccgatt tcgcaaagcg gagcacgact gtataatccc
     2461 gccgcgcttt tttcggccgg aggccgagtg actatcgccg gatggggact tgaatcctcg
     2521 ttctatctgg ggctccctac ccgagaagca ggctatctgc tgcttgggct ctacctgctg
     2581 gtggtcgcgc tcaccgttcg caagccggcc attcggctgc cgagaaagct cggttggctg
     2641 ctgttcgcat cggccgggct gctgctgggg gcgttcgtgc tttccggcgc gattaccatc
     2701 cgaatcttga ctgcggctgc ggctcctgaa cagttgcagg tgggtgccgg ctcaaccttt
     2761 ccgctgctcg gtgcgactcc ctggatgatc gcgggtggct tttttggaga tcttcccgct
     2821 gtactgttgg ggctgctcgg aggcctggtc cgcggagggt ggggtaccca gcgactgctg
     2881 accccctttg gaatagcagc ccaggcagcc ctgttcagct ggttgatccg gcgcccctat
     2941 tcagaatggc ctggccgtct cgcgcggcgt ccgctgccta gcgcgctggc aacaggtctc
     3001 tttttcggtt ttctgcgctc gctcgaagac ttcgtctatt tgcccgggga cttcttgggt
     3061 gcattagatc agactacggc gaccctcggg ccgaccgtgc tggcctcggt gctcgaactg
     3121 ggacttgccg gaatattgac cgccggcctt aaggcggcgt ttgccagtca ctggcaagct
     3181 cctcggcacc tgatcctggg cccctacaac cggactctgg cggcgcggtt ggttaccgtg
     3241 atggtgtcct tgggccttgc gggaggggcc gcgctcacct acggcaattg gctgctctcg
     3301 cgggcctccg ctcagcgcat catctcagac cagatgcttc agaccgcctc acaagtggcc
     3361 gagggaatcc cattcttcat ccagaccggc cggaccgaga tccgatcgat agccgagagc
     3421 ctaccggagc aaggcgctgc tcgggagatc tcagctgccg ccctcaccga ggcgatggcg
     3481 gaccgggcct acttcacccg gctggcagtc ctggattccg gaggagctgc ttgggccgca
     3541 tggcaaggat cgggttccgc cctgccgcgc gagatcgaag ttgccgctca gaccggcgcg
     3601 cctcaagaat tggtggtcgg tggcgagctt ccaggcgagg gggtccaact ggtctttctc
     3661 accccgttgg ctagcagtag cgaagggcca ggcatgctgg tcggatggac cgatcttgat
     3721 cacaatccga ttctggagcc gctcaacgcc cggctccagg cctataccgc cggggaagct
     3781 ttggtagtcg acaaccgtgg cagggtgatc ctgcaccccg accccggcca gtggctgcgg
     3841 ccgatcatct acccggatcc ggcgagcgcg gctttctttc acgcctccga ttcgactggc
     3901 agccgatcct gggtctactc acttcaggtc gagggttacc catggagggt gattgtcagc
     3961 actcctcaaa gtgcgctgga tgcgctggcg ctctcaacca ccgtgaatct tttcctgctg
     4021 ctggtgagcc tgggaggagt cttgatcgcg gcagttcacc tgatgagtcg ccggctcacc
     4081 cggccgctgc gtgacatggc cggcacggcc gaagccatcg cgctgggtga tctcgaccgc
     4141 caggtgccgg ccgggggcga ggatgagatt ggccgattgg ccggctcgtt cgagcggatg
     4201 cgccaggcgc tgaaggcgcg cctcggcgag ctgaatctgt tgctggcaac cagccgccgg
     4261 atggccgaga gttttgacct cgcggaggcc cttccgattg tgcttaatcg agttcgagat
     4321 ctgatgaacg tcgattgcgt ccggctggtg ctgagcggct cagaggctga actcgaaacc
     4381 tatcaggcgg gcattctcgg taagggttgg gagagcctcg acctggggat cctggagcta
     4441 acccgcaagc gcgggcagtt ttcactggag aatccggccc gggccggggc ggtgctggac
     4501 ctcggcggaa tctcagacgg gttggaggcg ctgatcgcct tcccggtcaa acatgaagct
     4561 gacgaccttg gcgcgctctg gctcgggcac ggccatcccc gggcgtttac aaagtcagag
     4621 gtccggttgc tgtcgatctt cgccgggcaa cttgcgatct cgctcgagaa cgtcggcctc
     4681 tatcgccggg ccgagcgaga gcgaagccgg ctgttggcaa tcctcagctc gactcccgac
     4741 gcggtgatcg tcacggacgc ccgggggcgg gtgtcgttgg ccaatcctgc tgcagatgcg
     4801 gtgctgacga cgagggcaga agatgcaatc ggtcggccta tcggcgagct ggtgcgggtc
     4861 cctgagctgg ccgccctctt caccgaaccg ctccctccca gccgctcgct tgagatcaag
     4921 atcgatggag ggcgggtgct gtatgcctca gcctcggatg ttccgggcgg gcagctcggc
     4981 agggtgtgcg tcctttcgga catcacccac tacaaagtcc ttgatgccct gaagtccgag
     5041 ttcgtctcta cggtgagcca tgacctgcga tcgccgctca cgctgatgcg gggctattcc
     5101 agcatggttg ccaacgtcgg atcggtaact agccagcagc aggagctgct agacaagatc
     5161 cagaccagcg tggatcgaat gaccaaactg gtggacgacc tgctcgatct ggggcgaatc
     5221 gagggcggga ttggcctcag ccttgaagcg gtcgaaatat cggcagtcct ccaagaagtc
     5281 ttgaccagtt tccgacctct ggcgctcaat aagcaggttg cgctcgaagt gcagctcggt
     5341 tcaagcttgc ttccggtgca ggccgatcca atcttgctgc gccaggcgat cgccaacctg
     5401 gtggacaacg cgatcaagta ttctcagccc ggtgcccgcg tgtcattgcg ggccgatcaa
     5461 gtcgaaggcc gccagatcgt tcaggtccaa gactcgggcg tcggtgtcgc ttcagccgac
     5521 cagcctcggc tcttcgagaa gttcttccgg gtgggagagg gcaagggggc cggccttggg
     5581 ctcgccattg tcaaatccat agtcgaacag catcaaggcc gggttagcgt ggagagtcga
     5641 ttgggccgcg ggagcacttt caccatcgcc ttcccgatgc gggtggaacg ggtccgggcg
     5701 gcggcctgaa atgccccagg aggtgcttct cg
//
LOCUS       DATDPQ010000375         4410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2331712, whole genome shotgun sequence.
ACCESSION   DATDPQ010000375 DATDPQ010000000
VERSION     DATDPQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331712"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VI520_08375"
     CDS             complement(<1..299)
                     /locus_tag="VI520_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLF37933.1"
                     /translation="MVTLEIRDLHASVEEKPILKGVNLIVKQGEIHGLMGPNGAGKST
                     LANVLMGHHSFQVTQGRVLFKGQDILELGPDERARMGLFLAFQYPVAIPGVTVAN"
     gene            513..1157
                     /locus_tag="VI520_08380"
     CDS             513..1157
                     /locus_tag="VI520_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HLF37934.1"
                     /translation="MASPTRDLVLRTLRARGKSTIRELAGAASVSPVSIRHHLSALQG
                     SGLVVSEEIKHGVGRPYLAFSLTEKALELYPGRYFRLTNRLLDEVKGHLPEGLVSRLF
                     SGVATAMADEYAAQLEGLPLRDRLPRLIEMLAAEGFDAEYEWQGDRVVIREFGCPYLQ
                     VGREHPEVCAVDTHFIATALDLPIERVNCLLDGDTHCTFSLKVLEDPEKLPIHG"
     gene            1150..1494
                     /locus_tag="VI520_08385"
     CDS             1150..1494
                     /locus_tag="VI520_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HLF37935.1"
                     /translation="MAEEVAPKAIWEADSTHPDQAIKVRSGLREVMDPELGLNVIELG
                     LIRDLRIQDSTQHVKMIMTTPFCPYGPALLEMTRARAEQAAGAATTIELAFEPWDPSM
                     MEEGVGAEWGLF"
     gene            complement(1504..1779)
                     /locus_tag="VI520_08390"
     CDS             complement(1504..1779)
                     /locus_tag="VI520_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37936.1"
                     /translation="MLGKKLNARKIESWLERSLVPVEPSPAFVSELRGRLVVLRGSRM
                     PSPWMVVLALSALLIIIVTWFGLGLRLVLALLGLIGLVERRRRSTEP"
     gene            complement(1789..2358)
                     /locus_tag="VI520_08395"
     CDS             complement(1789..2358)
                     /locus_tag="VI520_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLF37937.1"
                     /translation="MTARDGDEMDLVRRAGQDSEAFGELYSRHVGRIYNYIYYRTGDY
                     QDAEDLTARVFQRALHHVGSFQDQGVPFSAWLYRIAHNLVANWHRDQSRRPMVPLDDH
                     AIETRASSHPEVLAMAEEQNRLLLEAVRQLPGDRQQLLILKFVERQSNAEIGEVMGRT
                     EGAIKSLYHRTLNALRDQIKDSGEVRRKG"
     gene            2475..>4410
                     /locus_tag="VI520_08400"
     CDS             2475..>4410
                     /locus_tag="VI520_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HLF37938.1"
                     /translation="MQTLVALDLETTGLDPTRDAIIEIGAIRFRDDRIEEEWSQLVNP
                     GRPLPRFITQLTGITDEMLAEAPRLQEVLGTIQALVGDHPIVGHAVGFDLSFLQRKGL
                     FKENQPLDTYDLAAVVLPAAGRYSLAALANDLKVPRRTSHRALEDARTTMMVFRRLVE
                     LASELPYPILKALSEFGATIDWGAGWVFESALEGLGPQDEPAPLEFPAFSLPGERQPA
                     LLPKEAREPLDLEALAGLLQPGGAFSRAFPAYEHRTQQVRMLEAVGHALSEGRHLLVE
                     AGTGTGKSMAYLIPSFEWAARNGQRVLVSTNTINLQDQLIHKDIPDLNKALQRDYRAA
                     VLKGRRNYLCPRRLATLSRNGPRNAEEARVFAKLMVWLDAGGTGDVTEINLRGPAEAA
                     IWGRMSSDYEECGLEACLAYAGGTCPYYKAHLAAESAHVVIVNHALLLADIATGNRVI
                     PDYQFLIVDEGHQLEAATTRGLAFEAEEADLMRILQELEGTSLPRIPILAQQRLPADQ
                     LPQLQAKLATVGEQLRTASGLVRPFFQRLFDFVERRREGEPLTRYGQQERVLPATRSL
                     PLWTDVEIAWEGLRGPLGSLLESLTQISGAFEPSGEEDLAVGLRTLVRDLGTIVSSLD
                     ELIFQSDPQRIHWIELQGEPP"
BASE COUNT          835 a         1410 c         1333 g          832 t
ORIGIN      
        1 aagttggcga ccgtcactcc cgggatcgcc accggatact gaaaggccag aaacaagccc
       61 atccgagccc gttcgtccgg gccgagctcc agaatgtcct ggcccttgaa taatacccgg
      121 ccctgtgtga cctggaacga gtgatgcccc atcaagacgt ttgccagggt ggacttgccg
      181 gcgccgttgg gccccatcag gccgtggatc tcgccctgct tgacgatcag gttgacgcct
      241 ttcaggatcg gcttctcctc gaccgaagca tgcagatccc gaatctctag ggtgaccacg
      301 cgaccgattg ctcctaaaag gtcgaacgag ccgaattata gcatggggcc gatttttcgt
      361 caatattgtt tgaaaaatta gcataaatct acggagcatg accttgacat aagtggtcct
      421 gtgccgtggc ggggtgtgac gtcccttgga taataatgat aaatatcaga aatattgacg
      481 ccctgtgacc cgcatgctat actcaccgtt tgatggcttc gcctactcgc gacctggttc
      541 tccggacgct gcgggcccgc ggaaagtcca ccatccgcga gctggccggc gcagccagtg
      601 tttcccccgt atcgatccgt catcatcttt ccgccttgca gggaagcggc ttggtcgtct
      661 ccgaggagat taagcacggg gtcggacgac cctaccttgc gttcagcctg accgagaaag
      721 ccctcgagct gtaccccggg cgctatttcc gcctcaccaa ccggctgctg gacgaagtta
      781 agggccacct acctgagggg ctggtttcgc ggttgttctc cggggtagcg accgcaatgg
      841 ccgacgagta cgccgcccag ttggaggggc tgccgctcag agaccggctg ccgaggctca
      901 tcgagatgct ggccgcagaa ggcttcgatg ccgagtatga gtggcagggc gatcgagtag
      961 tcattcggga atttggttgc ccatacttgc aggtcggccg ggaacatccc gaagtatgcg
     1021 cggtcgacac ccatttcatc gccaccgccc tggacctgcc gatcgagcga gtcaactgct
     1081 tgctggacgg cgatacccat tgcaccttta gcttgaaggt gctcgaggac ccggagaagc
     1141 tgccgatcca tggctgaaga agtcgcgccg aaggcgatct gggaggccga ctccacccac
     1201 cccgatcagg cgatcaaggt ccgctcaggg ctgcgtgaag tcatggaccc tgagctcggc
     1261 ctaaacgtga ttgaactcgg gttgatccgc gatctccgca tccaagattc aacccagcat
     1321 gtcaagatga tcatgaccac ccccttctgt ccgtatggcc cggccctctt ggaaatgacc
     1381 cgggcccggg ccgaacaggc tgccggggcc gccaccacca tcgagcttgc cttcgaaccg
     1441 tgggacccaa gcatgatgga ggagggggtg ggcgcggagt ggggactctt ttagcggctc
     1501 ggcctacggc tccgtgcttc gccgcctacg ttcgaccagc ccgatcagac cgagcagggc
     1561 cagcaccaat cgcagaccga gcccaaacca ggtcacgatg atgatcaaca gggcgcttag
     1621 cgccaagacg accatccaag gcgacggcat ccggctgccg cgcaggacca ccagccgccc
     1681 ccgcaactcg ctcacaaagg ccgggctggg ctccaccgga acaagcgagc gctctagcca
     1741 ggactcaatc ttccgggcgt ttaacttctt cccaagcacc atcgcagcct accccttgcg
     1801 gcgaacctcc cccgaatcct tgatctgatc tcgcagggca ttgagggtcc ggtgatacag
     1861 gctcttgatc gctccctcgg tacgccccat aacttcaccg atctcggcgt tcgactgccg
     1921 ctcgacgaat ttcaagatca aaagctgctg ccgatctccc ggcagttggc gcactgcctc
     1981 caatagcagc cggttctgct cctccgccat cgccaggacc tctggatggc tgctcgctcg
     2041 ggtctcgatc gcatgatcat ctagcgggac catcgggcgc cggctctgat cgcggtgcca
     2101 attcgccacc agattgtggg cgatccggta tagccaggcg gagaacggca ctccttggtc
     2161 ctggaaactg ccaacgtggt gcagtgcccg ctggaacact cgggcggtca gatcctcggc
     2221 atcctggtag tcgccggtcc ggtaatagat gtagttgtaa atccgcccaa cgtgccggct
     2281 gtaaagctcc ccgaaggcct ccgaatcctg gcctgcccga cggaccagat ccatctcgtc
     2341 gccatcccgg gcggtcatct ttcgggctcg cccgagtcat taatgaaaac acctgacatg
     2401 ctgggtagtt gccctagagg aaggagcgcg cagtatagca tacgggtcgg atgatataat
     2461 cccgcccccc gcgaatgcag acgctcgtcg ccctggatct cgagacaacc ggtctggatc
     2521 caacccgtga tgccattatt gagattggcg cgattcgatt ccgggacgat cggatcgaag
     2581 aggaatggag ccagctggtc aatcccggcc ggccgttgcc ccgcttcatc acccagctca
     2641 ccggcattac cgacgagatg ctggctgagg caccgcgcct gcaagaggtt ctgggaacga
     2701 tccaggcgtt agtcggggac catccgatcg tcggtcacgc ggtcggcttt gacctgagtt
     2761 tcctgcagcg caagggtctg ttcaaggaga atcagccgct ggacacctac gatctggccg
     2821 cggtcgtgct gcccgccgcc ggccgctaca gcctggctgc cctggcgaac gatttgaaag
     2881 ttccgcggcg gacctcccat cgggcgctcg aagacgcccg gaccaccatg atggttttcc
     2941 gacgcttggt cgagctggcg tctgagctgc cctaccccat cttgaaggcg ctgagcgagt
     3001 tcggggccac tatcgattgg ggagcgggat gggtgttcga gagcgccctc gaagggctag
     3061 gtcctcagga cgaacctgcg ccgctcgagt ttcctgcttt cagcttgccc ggcgaacggc
     3121 agcccgccct actgcccaag gaggctcgcg agccactgga cctcgaggcg ctggccgggc
     3181 tgctgcagcc gggcggcgct ttctcacgcg cctttcccgc ctacgagcac cgcacccagc
     3241 aggtccggat gctagaagcg gttggacatg ccctctcgga aggccgccat ctgctcgtcg
     3301 aagccgggac tgggaccgga aagtcgatgg cctatctgat cccaagtttc gaatgggccg
     3361 cccgaaatgg gcagcgggtg ctggtctcca ccaacacgat caacctgcaa gaccagctca
     3421 ttcataagga cattccggac ttgaacaagg ccctgcagcg cgactatcgg gccgccgtgc
     3481 tcaaaggccg gcgcaactac ctgtgcccac ggcgcctggc cacccttagc cggaacgggc
     3541 cccgcaatgc ggaagaggcc cgagtgtttg ccaaactcat ggtgtggctg gatgccggcg
     3601 ggaccggcga cgtcaccgaa atcaacctgc gtggtccggc ggaggcggca atctggggcc
     3661 ggatgtcttc cgactacgaa gagtgtggcc tggaggcctg tctcgcctat gccggcggga
     3721 cctgcccgta ctacaaggcc cacctggcgg ccgagagcgc ccacgtagta atcgtcaacc
     3781 acgccttgtt gctggccgat attgccaccg ggaacagggt aatcccggac tatcagtttt
     3841 tgatcgtgga tgaaggccat cagctggaag ccgcgaccac ccgcgggttg gccttcgagg
     3901 ccgaggaagc ggacctgatg cggatcctgc aggaattgga gggaaccagc ctgccccgga
     3961 tccctatcct ggctcaacaa cgtctgccgg cagatcagct tccccagttg caggccaagc
     4021 ttgctaccgt cggcgagcag ctgcgcaccg cctccggcct cgttcggcct ttcttccaac
     4081 ggctgttcga ctttgtggag cggcgccgcg aaggggaacc gctcactcgc tatggccagc
     4141 aggagagagt gctgcctgcc acccgcagtc tgccgctctg gaccgatgtg gagatcgcct
     4201 gggaggggct gcgcggtccc ttgggcagcc tgctggagtc gctcactcag atcagcgggg
     4261 cgttcgagcc gtccggggag gaggacctgg ccgttggatt gcggactctc gtgcgcgatc
     4321 tggggacgat cgtgtccagc ctggacgaat tgatctttca gtctgacccg cagcgaatcc
     4381 attggatcga gctgcagggc gaaccacccc
//
LOCUS       DATDPQ010000376         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1788792, whole genome shotgun sequence.
ACCESSION   DATDPQ010000376 DATDPQ010000000
VERSION     DATDPQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788792"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VI520_08405"
     CDS             <1..225
                     /locus_tag="VI520_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37939.1"
                     /translation="MGIALPTRELPTDPLDRLVKVRDHTLANLSNLATRIGVVALSGE
                     TWSARKVMRRTLWHERDHTDHISKLKLRLR"
     gene            286..1059
                     /locus_tag="VI520_08410"
     CDS             286..1059
                     /locus_tag="VI520_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLF37940.1"
                     /translation="MTYTAIKTEIHGRAGLVQIHRPEAINALNSTVMEEIASALSNFD
                     ADPQIGAMVLTGDGRAFASGADIKEMAEASAVDMLTADRIGRWDRIRGVKKPVIAAVS
                     GWCLGGGNELAMLCDMIVASETARFGQPEINLGVMPGAGGTQRLTRAVGKALAMEMVL
                     NGRNLTAEEALRVGLVNRVVPVELYLEEALALANEIAARAPIAVRLAKESVNHAFESF
                     LADGIADERRSFYFLFSTEDQKEGMKAFSERRSPNWRGR"
     gene            1076..>2133
                     /locus_tag="VI520_08415"
     CDS             1076..>2133
                     /locus_tag="VI520_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLF37941.1"
                     /translation="MRAIYFDKAIPRALVTKALRPLWPGVVWSPLSPSGVEQVPDPPL
                     PGPQWLRVRNRQCGICATDLSLLYVRADPRIAPAALPGIRRIYLGHEAVGEVNEVGPA
                     VDRFRVGDRVVIEARPVGSPNCHTQVIDPPCRFCARGDTRLCENGSLGKGPVGAGGGW
                     SDSYTAHQSEVWPVPDDLNDDQASLIEPMAVAVHGVLRRPPAPGDRVLILGAGIIGLL
                     TLQAVKALAPQAHVTVLVRYAHQGQAAKRLGADEVMSDRGDIYAKSAEITGAKYYRGQ
                     FNRGMLLGGYDILYDCVGDRSTLRDSLRLARAGGAVVLVGISLNPLSLDTTPVWFQEV
                     DLVGAKTFGMEALNGRTL"
BASE COUNT          372 a          661 c          743 g          357 t
ORIGIN      
        1 atgggaattg ccttgccgac ccgcgagctt ccaaccgatc cactcgaccg cctggtgaag
       61 gtccgcgatc acaccctggc caacctctca aacctggcta cccggatcgg ggtcgtggcc
      121 ctttccgggg aaacctggtc ggcccgcaag gtcatgcgcc gaaccttgtg gcatgagcgc
      181 gaccataccg atcacatctc caaactcaaa ctgcggctcc gctgaggcgg accgtcgggc
      241 cgcgccgatt gcatccccta atccattgtt gcaccggagg gtggcatgac ctataccgcg
      301 atcaagactg agatccacgg ccgggccggt ctggtacaga tccaccggcc cgaggcgatc
      361 aacgcgctca actcaaccgt gatggaggaa atcgccagcg cgctgagcaa cttcgacgcc
      421 gatccgcaga tcggggccat ggtgctgacc ggggacgggc gggcctttgc ctcgggggca
      481 gacatcaagg aaatggcgga ggcctcggcg gtggacatgc tgaccgccga tcggattgga
      541 cgctgggacc gcatccgcgg cgtcaagaag ccggtgatcg cggcagtctc cggatggtgc
      601 ttgggcggcg gcaatgaatt ggcaatgctg tgcgacatga tcgtggcctc cgagaccgcc
      661 cggtttggcc agccggaaat caacttggga gtcatgccgg gggcgggcgg gacccaacgg
      721 ctgacccggg cggtgggcaa ggcgctggcc atggagatgg tgctcaacgg ccggaatctg
      781 accgctgagg aagcgctgcg ggtgggactc gtcaaccggg tcgtgccagt cgagctgtat
      841 ctggaagagg cgctggcgct ggccaacgag atcgcggcgc gggccccgat cgcggtccgg
      901 ctggccaagg agtcagtgaa ccatgccttc gagagcttcc tggcggacgg gatcgccgac
      961 gagcgccgta gtttctattt cctgttcagc accgaagacc agaaagaggg aatgaaggcc
     1021 ttcagcgaga gacgttcccc gaactggagg gggcgctgag cagggcagga gccggatgag
     1081 agcgatctac ttcgacaaag cgatcccccg ggccctggtc accaaggcct tgcggccgct
     1141 gtggcccggt gtcgtgtggt cgccgctctc accctcggga gtagagcagg ttcccgatcc
     1201 gccgctgcca ggcccgcagt ggctgcgggt gcgcaaccgc cagtgcggga tctgtgctac
     1261 ggacctctcg ctgctttatg tgagagccga tccgcggatc gcgccggcgg ccttgcctgg
     1321 aattcggcgg atctatctgg ggcacgaggc agtaggggaa gttaacgagg tcggcccagc
     1381 cgtcgatcgg tttcgggtcg gtgatcgggt ggtgatcgaa gcccggccgg ttggaagccc
     1441 aaactgccac acccaggtga tcgatccgcc ctgccgcttc tgcgcccgcg gtgacactcg
     1501 actttgtgag aacggctcgc tggggaaggg acccgtgggg gccggcgggg gatggagtga
     1561 cagctacact gcgcaccaga gcgaggtctg gccggtcccg gatgacctga atgacgacca
     1621 ggcttccctc attgagccga tggccgttgc ggtccatggg gtcctgcgcc ggccgcccgc
     1681 gcccggagat cgggtgctga tcctgggggc cgggatcatc ggcctgctca cgctgcaggc
     1741 cgtgaaggcc ctggcacccc aggcccatgt gacggtcttg gtccgttacg cccatcaagg
     1801 ccaggccgcc aagcgactgg gagcggacga ggtgatgagc gaccgagggg acatttacgc
     1861 caagtcggcc gagattaccg gagccaagta ttaccggggg cagttcaacc ggggcatgct
     1921 gcttggcgga tacgacattc tctacgattg cgtcggcgac cgttccacct tgcgcgactc
     1981 gctccggctg gcccgggcgg gcggcgcggt cgtgctggtc ggcatctcgc tcaacccgct
     2041 cagcctggat accaccccgg tctggtttca ggaggtggac ctggtcggcg ccaagacctt
     2101 tgggatggaa gccctcaacg gccgaacgct gca
//
LOCUS       DATDPQ010000377         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1016313, whole genome shotgun sequence.
ACCESSION   DATDPQ010000377 DATDPQ010000000
VERSION     DATDPQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016313"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1485)
                     /gene="hydA"
                     /locus_tag="VI520_08420"
     CDS             complement(43..1485)
                     /gene="hydA"
                     /locus_tag="VI520_08420"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HLF37942.1"
                     /translation="MQALRHGVFPVLIRGGTLLTIRGEQRADLRIAGGRIAEVGAELP
                     AGNAQVIDATGKLVMPGGVDPHTHFDLPMFDTVSSDDHYTGLRAAAFGGTTTVLDFVP
                     LIRPTLAESIEAWQAKAEPNAAIDYGFHMNITRLDDGVRREIPLLADRGITTLKVFTA
                     YNGRLRLQDGEIFEVMRIAREHGLLTMLHAENGDVIEILTAEAVAAGHSEPEWHALSR
                     PAWGAVEAVLRGIALAEQAGAPLYVVHLNTAGELDQIRYGRARGARVMAETCPQYLFF
                     NLQHLKRPDGAKWVCSPPVRTEQDNAALWSGLSDGSLQTVGTDHCPFFYDGRRSIDYE
                     GAPVAIPGKELGARDFSKIPNGLPGVGDRMPILWTSGVGQGRINPAQFVALTSTNPAK
                     IFGLYPQKGELLPGSDADLVIWDPARRLNYGLAHSHHRTDYNLYEGWELVGFPERVFL
                     RGQLIVDGDRWLGRRGQGRFLKRSAGAEVL"
     gene            complement(1449..1967)
                     /locus_tag="VI520_08425"
     CDS             complement(1449..1967)
                     /locus_tag="VI520_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37943.1"
                     /translation="MGSGRSGAMILIVLGIAIAGIGTLYVVSQLSSGELQTGGAVVGI
                     GIFGLPALLAIGIGASLLARSRQVAAGDMEQAQLRKILDMVKSKGQVPISELVIDLGS
                     TRKAVQDQVHSLVGMGLFSGYINWDEGTLFSAEAANLRELERCKHCGGEVSFVGKGVL
                     ACKHCGTEYFLS"
     gene            complement(1970..>3065)
                     /locus_tag="VI520_08430"
     CDS             complement(1970..>3065)
                     /locus_tag="VI520_08430"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HLF37944.1"
                     /translation="LIYFVYYARGRDPKVGPVPEYLAEPPGDLPPGIMGTLLDEQAEM
                     RDVVATIVDLARKGSLRIEETANKGLLGLGSTDFIFHRQAAAADGLNPVESEIVSGLF
                     KGGKTSVEMSDLREKFYTRLPAIQSKLYQEMVNRGFFRTNPDTTRKLWRGLGGAVLAG
                     AFFLGIFASGALSSYTSLLPCVFGALGLTGLVALVAGGAMPAKTRKGAEAAAQWTAFK
                     TYLERIDKLADLQQVGELFERYLPYAIALGIKDSWIHKFSQQPSTPVPGWYMPYYGGG
                     SARRTVSAGGKGGGAAVPPLHGPGGLQGMSEGLAGGLQSMSDGLTRMLNSAGRVLQSA
                     PSSSGRSGGGFSGGGFSGGGGGGGGGRGFG"
BASE COUNT          557 a         1060 c          912 g          536 t
ORIGIN      
        1 cgaccgccga tccgaggtga cccgattgta gcatgaaccg gatcacagca cttcagctcc
       61 cgccgaacgc ttgagaaagc gtccctggcc ccgccggccg agccatcgat ccccatccac
      121 gatgagctgc cctcgcagga aaactctttc ggggaatccc accagctccc atccttcgta
      181 caggttgtag tcggtccggt gatgggagtg ggccaggccg taattcagcc ggcgggctgg
      241 atcccagatc actagatcgg cgtccgaccc gggcagcagc tcgcccttct gcggatagag
      301 gccgaagatc ttggccgggt tggtgctggt cagcgcgaca aactgggctg ggttgatgcg
      361 cccctgcccc accccgctgg tccacaggat cggcatccga tctccgaccc ccggcagacc
      421 gttggggatc ttgctgaagt ctcgggcgcc cagctccttg cccggaatgg cgaccggcgc
      481 accttcatag tcgatcgagc gccggccatc gtagaagaag ggacaatggt cggtgccgac
      541 ggtctgcagg ctgccgtcgc tcaggccgga ccagagggct gcgttgtctt gctcggtccg
      601 caccggcggc gagcaaaccc acttcgcgcc gtccgggcgc ttcagatgct ggaggttgaa
      661 gaacaagtac tgggggcagg tttcggccat caccctggcg ccgcgagccc gcccgtagcg
      721 aatttgatcc agctccccgg cggtgttgag gtggacgacg tacagcggcg caccggcctg
      781 ctcggccagg gcgatccctc gcagcaccgc ctcgaccgct ccccaggccg gccggctgag
      841 cgcgtgccac tccggctcgg aatgcccggc ggccacggcc tcggcggtaa ggatctcgat
      901 cacgtcaccg ttctcggcat ggagcatggt cagcaagccg tgctcacgcg cgatccgcat
      961 cacttcgaag atctcaccgt cctgcaggcg cagccggccg ttgtaggcgg tgaacacttt
     1021 caacgtggtg atcccacgat cggcaaggag cggaatctct ctgcgcactc catcatccag
     1081 ccgagtgatg ttcatgtgga atccgtagtc gatcgccgcg ttgggctcgg ccttggcctg
     1141 ccaggcctcg atcgattcag ccagggtcgg ccggatcagc ggcacgaaat cgagcacagt
     1201 tgtagtgcca ccaaaggccg ccgccctgag accggtgtag tggtcatccg aggaaaccgt
     1261 gtcaaacatg gggaggtcga aatgggtgtg ggggtcgaca ccccccggca tgaccagctt
     1321 gccggtggca tcgattacct gggcgttccc cgccggcagt tccgcgccca cctcggcaat
     1381 ccgcccgccc gcgattcgca ggtccgcccg ctgctcgcct cggatcgtca ggagagtgcc
     1441 gccccggatc aggacaggaa atactccgtg ccgcagtgct tgcaggccag cacaccctta
     1501 ccgacgaaac tcacttcgcc gccgcagtgt ttgcagcgct ctagctcacg caggttggcc
     1561 gcctcggctg agaaaagtgt gccctcgtcc cagttaatat agccgctgaa cagccccatt
     1621 cccaccaatg agtggacctg gtcctggacc gctttgcggg tcgatcccag gtcgatgacc
     1681 agctcgctga tcgggacctg acctttgctt ttcaccatgt ccaggatctt ccgcagctgg
     1741 gcctgctcca tatcgcccgc cgctacctgc cgcgagcggg ccagcagcga tgcgccaatg
     1801 ccgatcgcca ggagggctgg cagaccgaag attccgatac caaccaccgc tccgcccgtc
     1861 tggagttcgc cggaactcag ctggctcact acgtacagcg tcccaatgcc cgcaatcgcg
     1921 attccaagca caatcagaat catcgctccg cttcttccgc tacccatcgc tagccgaatc
     1981 ctcgcccacc gcccccgccg ccgccacccc cgctgaaacc cccaccgctg aatccgcctc
     2041 cgctccggcc gctcgaggag ggggcggact gcagcacccg gccggcgctg ttcagcattc
     2101 gggtcaatcc gtcgctcatg ctctgcaagc cgccggccag accttctgac atcccttgca
     2161 agcctcccgg accatgcagc ggaggaaccg cagcgccgcc gccctttcct ccggccgata
     2221 cagtccgccg agctgatccg ccgccgtagt acggcatata ccagcccggc actggagtgc
     2281 taggctgctg ggaaaacttg tgaatccaag aatccttgat gccgagcgcg atcgcgtacg
     2341 gcaggtagcg ttcgaatagc tcaccgacct gctggaggtc cgccagcttg tcgattcgct
     2401 ccaaataggt cttgaaagca gtccactggg cggcggcctc ggcgcccttg cgggtcttgg
     2461 ccggcatcgc ccctccggcg accagcgcga ccagaccggt gagaccgagg gccccaaaaa
     2521 cgcaggggag caggcttgtg tagctcgaaa gcgcgcccga ggcaaagatc ccgaggaaga
     2581 aggccccagc cagcaccgca cctcccaggc cgcgccagag cttacgggtc gtgtccggat
     2641 tggtgcggaa gaatccgcgg ttaaccatct cctgatagag cttgctttgg atcgcaggca
     2701 gccgggtgta gaacttttcc cggaggtcgc tcatctcaac cgaggtcttc ccacccttga
     2761 agagcccgga cacgatctcg ctctcaaccg gattgaggcc atccgcagcc gcggcctgcc
     2821 ggtgaaagat gaaatcggtg gagccaaggc ccagcaaacc tttgttggcg gtctcttcga
     2881 ttcgtaagct ccctttgcga gccagatcca cgatcgttgc caccacgtcc cgcatttcgg
     2941 cctgctcgtc cagcaaggtg cccatgattc caggtggcag atcgccaggc ggttccgcca
     3001 agtattccgg caccgggccg accttaggat cccggccgcg ggcgtaatac acaaaataga
     3061 tcagg
//
LOCUS       DATDPQ010000378         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3610558, whole genome shotgun sequence.
ACCESSION   DATDPQ010000378 DATDPQ010000000
VERSION     DATDPQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3610558"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VI520_08435"
     CDS             <1..409
                     /locus_tag="VI520_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HLF37945.1"
                     /translation="NLGRVVQIGSPAELYEHPASPFVAGFVGVSNLISGELARKITAS
                     PKTFSVRPEKISIQSLEQLAPSGYSSVEGQVRDVIYLGIHTRYLVELDSGEDLTVVEQ
                     NRDTTSMDVLSARGRRVRLVWNPVHNQRVEESA"
     gene            419..1402
                     /locus_tag="VI520_08440"
     CDS             419..1402
                     /locus_tag="VI520_08440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37946.1"
                     /translation="MRPSPAALRGEARVTASGLRQETHRPLLHRLGTFIYLRPWLVLA
                     LLLGPPLLWMVVIYLGSVASLVVQSFYSLDPFTGQVIREFTLATYRELFVPAHLEIFL
                     RTALMAAAVTIATAILAFPFAYFMARFASRRAKAVLYLAVLLPLWSSYLVRVYAWRLI
                     LAKQGILNWFLERLNLLGVLEAALRIPVIGGPSLSVSFLGMFIVFVYVWLPYMILPIQ
                     AALERVPQSLLEASGDLGARPRRTLRHVTLPLAFPGVLAGSIFTFSLTLGDFVVPYSL
                     GNSSYFIGQAVLAFTGTAFNIPLAAAFTVVPMVIMGFYLVGARELGAFEAL"
     gene            1389..2189
                     /locus_tag="VI520_08445"
     CDS             1389..2189
                     /locus_tag="VI520_08445"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37947.1"
                     /translation="MKRSEPAAHSPLGLRIMTLGVLLFMHLPMGIILLYAFTTDESTF
                     VFPPPGLTTDWFRVAVGRADVWRALGLSMRVAGVSTLVAIFLGTLAAAAVYRAKFFGR
                     EAISFALILPIALPGIVSGIALRSAIGILNVPFSFWTIVIGHATFCMVVVYNNVLARF
                     RRTGSSQIEASMDLGANPFQTFRHIVLPAIATALLAGGILAFALSFDEIVVTTFTAGQ
                     EQTLPIWMFSQLTKPRARPVTNVVAVFVVAITTIPILLANRLTERTIE"
     gene            2264..2611
                     /locus_tag="VI520_08450"
     CDS             2264..2611
                     /locus_tag="VI520_08450"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLF37948.1"
                     /translation="MVIYQTPQLDLLFGALANATRRAILDRLTSSDQSVLELVGLFEM
                     SQPAVTKHLNVLQQAGLIRRRKEGRYRYCSLQGDALETAEEWIERTREYWEKSFAALD
                     EYLEETQNKEDQA"
     gene            2608..3075
                     /locus_tag="VI520_08455"
     CDS             2608..3075
                     /locus_tag="VI520_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLF37949.1"
                     /translation="MSATTQAPIAVLEMTRSFSAPVEKVYRAFTESPAVARWGCGRSY
                     ENLAIDLDLRPGGVLHHRARHIATGVEWTFFGVYEQVIPNERLLYTFDWKTDWRQAAK
                     PSLVELRFHSMGKRTEVELVHSRLPDPAIATSEQHWNEFLQVLEELLVRQELF"
     gene            3093..3263
                     /locus_tag="VI520_08460"
     CDS             3093..3263
                     /locus_tag="VI520_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37950.1"
                     /translation="MYDPILLRAIAQARQTDLAPHHRQRSHLPSRIFRSARTMLLLAL
                     SFTAGWALGAFV"
     gene            complement(3475..3645)
                     /locus_tag="VI520_08465"
     CDS             complement(3475..3645)
                     /locus_tag="VI520_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37951.1"
                     /translation="MTSASGKSKVAVGVTVGVGVMEGVSVMVGVFVTVGVNVAVGVVL
                     GVGVEVAVGVGV"
BASE COUNT          637 a         1176 c         1088 g          787 t
ORIGIN      
        1 caacctcggc cgggtcgtgc agatcggatc accggccgaa ctctacgagc atcccgcctc
       61 tccctttgta gcgggtttcg ttggagtgtc caacctgatc agcggcgagc tggcgaggaa
      121 gatcactgcc tcaccgaaga ccttctccgt tcggcccgaa aaaatcagca tccaatcact
      181 cgaacagctc gccccgagtg gatacagctc ggttgagggt caggtgcggg acgtgattta
      241 tctggggatc cacacccggt acttggttga actggacagc ggagaggatc taacggtcgt
      301 cgagcagaac cgcgacacga cttcgatgga tgtgctgagc gcccggggcc ggcgggtgcg
      361 actggtttgg aatcccgtcc acaatcaacg agttgaagag agtgcttgag ctagaaatgt
      421 gcggcctagt cccgcggcgc tgcgggggga ggcccgagtg accgcaagcg ggctccggca
      481 ggagacccac aggccgcttc tgcaccggct gggcaccttc atctatctcc ggccttggct
      541 ggtattggcg ctgctcctgg ggccgccgct cttgtggatg gtggtgatat atctgggatc
      601 agtcgcttcg ctcgtggtcc aaagcttcta ttctctcgat cctttcaccg gccaggtgat
      661 ccgggagttc accctggcaa cttaccggga gttgttcgtt cctgcccacc tcgaaatttt
      721 cctgcgaacc gcgctcatgg cggccgcagt gacgatcgcg actgccatcc tggcatttcc
      781 cttcgcgtac ttcatggccc ggttcgcctc gcgccgggcg aaggcggtgc tctacctggc
      841 cgtgctgttg ccgttgtggt cttcctacct ggtgagggtg tacgcctggc gcctcatcct
      901 ggctaaacaa ggaatcttga actggttctt ggagaggttg aatctcctgg gcgtgctgga
      961 ggccgcgctg aggattccgg tgattggcgg cccgtcgctc tcggtttcct tcctggggat
     1021 gttcatcgtg ttcgtgtatg tctggttgcc ctatatgatc ctgcccatcc aggctgccct
     1081 cgaacgggtg ccgcaatcgc tcctggaggc ctccggcgat ctgggagccc ggccgcgccg
     1141 caccctgcgc cacgtcacat tgccgctggc tttccccggg gtcctcgctg gctcgatctt
     1201 cactttttct ctgacgcttg gcgactttgt tgtgccttat tcgctcggca actccagtta
     1261 cttcatcggc caggcggtgc ttgcgttcac cggcacggcc ttcaatattc ctctagcggc
     1321 agccttcaca gtcgtgccaa tggtgatcat gggcttctat ttggtcgggg cccgggagct
     1381 cggtgccttt gaagcgctct gagccggcgg ctcattcccc actggggctc cggatcatga
     1441 ctttgggcgt gctgctgttc atgcacttgc cgatggggat tatcctgctc tacgccttca
     1501 cgaccgacga gtcgaccttc gtcttcccgc cgccgggcct gacgaccgac tggttccggg
     1561 tcgcagtggg gcgagcggat gtttggcggg ccctcgggtt gtccatgcgg gtggcggggg
     1621 tctcgaccct ggtcgccatc tttctgggaa cgttagccgc ggccgccgtc taccgggcca
     1681 agttctttgg ccgggaggcg atctcctttg ctctgatcct gccgatcgcc ctgccgggaa
     1741 tcgtgagcgg gatcgcgctt cgctccgcga ttgggatcct gaacgtgccc ttcagcttct
     1801 ggaccattgt tatcggtcat gccaccttct gcatggtcgt tgtctacaac aacgtgctgg
     1861 cccggttcag acgaacgggc agttcgcaaa tcgaggcctc gatggacctg ggagccaatc
     1921 cgtttcagac ctttcgccat atcgtcctgc cggcgatcgc caccgcgctg ctggccggag
     1981 gcatcttggc ctttgcgctg tcttttgacg agatcgtcgt cacgaccttc accgccggcc
     2041 aggagcagac ccttccaatc tggatgttca gccagctgac aaagccgcgg gcgcggccgg
     2101 tcactaacgt cgtagcggtc tttgtcgtag ccatcaccac catccccatt ctgcttgcca
     2161 atcggctgac cgagcggacg atcgagtagc gcctccggcg caagctctcc gctcacgaac
     2221 tgggtattga caaggtcgat tcctgtgcta taaacataac taaatggtta tatatcaaac
     2281 gccgcaactg gatctgctat ttggcgcctt ggccaacgcg acccgccggg caatcctaga
     2341 ccggctcacg agctctgatc agtccgtcct ggagctcgtt ggcctgttcg agatgtccca
     2401 gccggcggtc accaaacact tgaatgtgtt gcagcaggct ggcctgatcc ggcgacgcaa
     2461 ggaggggcgc taccgctact gctcgctgca gggagacgcg ctggagaccg ccgaggaatg
     2521 gatcgagcgg acccgagaat actgggagaa gagtttcgcc gccctggatg aatacctgga
     2581 agaaacccaa aacaaggagg atcaagcatg agcgccacaa cccaagcccc gatcgccgtt
     2641 ctcgaaatga cccgcagttt cagcgcgccg gtcgaaaagg tgtatcgggc cttcaccgag
     2701 agcccagcgg tcgcccgctg gggatgcggc cggagttacg aaaacctggc gatcgacctg
     2761 gatctccgac cgggcggagt gctccaccac cgcgcgcggc acattgcgac cggtgtggag
     2821 tggaccttct tcggggtgta tgagcaggtc attccgaacg agaggctgct atacacattc
     2881 gattggaaga cggattggcg tcaggcggca aaacccagcc tggttgagct caggtttcat
     2941 agcatgggga agagaaccga agtcgaattg gtccactcgc ggctgccgga cccggcgatc
     3001 gctacgtccg agcagcactg gaacgagttc ttgcaggtct tggaagagct cttggtccgt
     3061 caggaacttt tctagtacca gggaagccag caatgtacga cccgattctg ctgcgagcca
     3121 tcgcccaagc caggcaaacc gacttggccc cgcaccaccg ccagcgcagc catttgccaa
     3181 gtcgcatatt tcgcagcgcg cgcactatgc tgttgctggc gctctccttc actgccggct
     3241 gggccctggg cgccttcgtg tagagaagcc caaagggttc attgcgaccc gaccggggct
     3301 ggcctagact acggggaggc cagccccggt gagtctcatc catccaggac ccgacgcaac
     3361 ccaattctcg cccgttcggg gcctttgcct gcttttgctg ggctgggtag cactggtcag
     3421 ctgcaatgcc ccgctcgccg caattccgat cctcggcgcg acggtgacct ccactcacac
     3481 tccaactcct acggccactt caaccccgac gccgagcact actccaaccg cgacattcac
     3541 cccgaccgta acgaacacgc ccaccatcac gctgactcct tccattactc cgacgccgac
     3601 cgtgacgccc accgccacct ttgacttccc agatgcggag gtcaatgtcc ccgccgctca
     3661 ttgccgctat gggccggaca ccgcctac
//
LOCUS       DATDPQ010000379         5796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_485068, whole genome shotgun sequence.
ACCESSION   DATDPQ010000379 DATDPQ010000000
VERSION     DATDPQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485068"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VI520_08470"
     CDS             <1..1576
                     /locus_tag="VI520_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HLF37952.1"
                     /translation="PIVGAIVNGELRELTYPIEMESQVRPVSMAGEDGMRIYRRSLTF
                     LLAATFEELHPGERLEVEHSVAHGGYHCQVKDRPQLSESELRNVEARMREFVRQDLPF
                     IKMQVPLQQAIDLFEAKGFKDKARLLANRRKPYLIMYQMGQFRDYHHGYMVPSTGFLN
                     WFALSPANEGFTLQFPRRETPTEVLPAPNYPKLLATFEQYSDWIETLGVSSVGALNDA
                     IQQGRTRELVLVSEALHEHRIGEMAAQIHAGGEAARLVLIAGPSASGKTTFSKRLSIQ
                     LLALGISPLPIGLDNFFVDREMTPRDEQGDYDYERLDALDLDRLNHDLGQLIAGQRVE
                     LPHYDFVAGRQMAGEVVQISKAHIIILEGIHGLNPALLPDIPGESAIRIYASPLTQLN
                     LDRYNRISTTDIRLARRLVRDASQRGKTAAETIARWESVRRGEKLYIFPYQENADMMF
                     NSALVYEGAALAPLAEPLLRQVTFGTNEHVEAKRLLSFLEWFVPVESSRIPDNSILRE
                     FIGGSILQDFRIWGAG"
     gene            1628..3172
                     /locus_tag="VI520_08475"
     CDS             1628..3172
                     /locus_tag="VI520_08475"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HLF37953.1"
                     /translation="MSETAIKLIGVLGAGTMGAGIALTALYAGHSVILAEVAPEALQR
                     GREYIEGHLARKGRSESLRQLQLSNDLQALAGSDVVIEAINEDLELKRTVFAELDRIC
                     PPPTILASNTSTLSITAIAAATRTPERVAGMHFFNPAPLMRLVEVARGAATAQATLDA
                     LIGLAERMGKTPVLTLDTPGFIVNRVARPFYGEALRLLAEQVAGHEAIDLLVTLGGGF
                     PMGPFELMDLIGLDVNLQAAQSMYDSSYGEPRYRPQSIQRRYVESGRLGRKTGVGFYQ
                     YPGGEKQWKAPEVPEPRQQSGFVLISDGSWGPEVGRLLVQAGYTLSETHGDLPLAALI
                     VNGREEGVRDVLRRYDRGLAPEIPILCQTVDIALSEVEQSVQQPQRLIGFDGLFFAAG
                     RAVTLVARPNLPDAARQAAETLVRSLGKLPVWVSESPALVLPRIVCCLANEAAFAVAE
                     GVAEPEAVDTAMKLGVNYPQGPLEWAATLGYRKVIAVLDHLADEYGEERYRVAPWLRN
                     RARRSL"
     gene            3217..4110
                     /locus_tag="VI520_08480"
     CDS             3217..4110
                     /locus_tag="VI520_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLF37954.1"
                     /translation="MTDRRFVGKPAAEFEFTAIEYEKADYRATITINRPKVLNAVNHA
                     VIEEMNVALKDASWDDSVAVIVLTGAGDRAFCTGADLREQEEFLERPRDYWKWMGEFI
                     ELHERLRNLGKPTVARLNGIVVGGGNEFNLSCDLAVAADDIYIRQVGTSHGSVALAGA
                     TQFLPLIVGDRRAREILLLNEEIPAAKALEWGLINYAVPRAELDRAVDELVAKLIAKL
                     PEKTRYTKQQLNFWRDLSWHLTIGHGREWLAIHNTSPETWEGVRAFGEKRAPDYDSIR
                     RRWAEDDSPEWLGGRPPRDPR"
     gene            4122..5051
                     /gene="paaA"
                     /locus_tag="VI520_08485"
     CDS             4122..5051
                     /gene="paaA"
                     /locus_tag="VI520_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HLF37955.1"
                     /translation="MIEAERLEAFEARIHRGEKIEPGDWMPDEYRRQLIRMISQHAHS
                     EIVGMLPEGAWITRAPNLRRKLVLLAKVQDEAGHGQYLYHAAETLGATREEMIEALLS
                     GRAKYSSIFNYPSLSWADMGVIGWFVDGAAILNQTMLARCSYGPYSRANIRISAEESF
                     HMKQGKEQVILMSQGTPLQRSMVQDAVDRWWWPTIMMFGPHDHDSPNSPVLIRWGIKT
                     KTNDQLRQDFVNQVAPEVLELGLSLPDPSLHYDEASGNWLTGSIDWDEFWRVVKGDGP
                     CNAERIAARRKAHADGEWVRAALAAYAERQATA"
     gene            5048..5587
                     /locus_tag="VI520_08490"
     CDS             5048..5587
                     /locus_tag="VI520_08490"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37956.1"
                     /translation="MRDTQWPRFQVFHQEHPDRPHTNAGAVHAPDAELALQNARDVFV
                     RRPDCVSLWVVPADQILSLTAEEIEHTDLPPAPAGNGDSQRYLVFIKRTQVGSHEHVG
                     AVAAASGPAALGEAVRRFGTADALVWWVVSESLIERTEAAEVGSLFGPAKDKPYRDQA
                     FYHTTTLMRQIRGRPKQEP"
     gene            5584..>5796
                     /locus_tag="VI520_08495"
     CDS             5584..>5796
                     /locus_tag="VI520_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HLF37957.1"
                     /translation="MKAEFRESLANYLTALGDDELILAHRGSEWAGHAPILEEDIAFA
                     NLALDELGHASTWYRLAAELLNEDPER"
BASE COUNT         1113 a         1779 c         1883 g         1021 t
ORIGIN      
        1 gcccatcgtc ggcgcgattg tcaacggtga gctgcgcgaa ctgacctacc cgatcgagat
       61 ggaatctcaa gtccgaccgg tcagcatggc gggggaagac gggatgcgga tctaccgccg
      121 ctcgctcacc ttcttgttgg cggccacctt cgaggaactt caccctggcg agcggctgga
      181 ggtcgagcac tcggtggccc acggtggcta ccactgccag gtgaaagacc gtccccagct
      241 ttcggaaagc gagctgagaa acgtagaagc ccggatgcgg gaattcgtcc ggcaggatct
      301 gcccttcata aagatgcagg tcccgctgca acaagccatc gacctcttcg aggcgaaggg
      361 gttcaaagac aaggcccggc tgctcgccaa tcggcgcaag ccctacctga tcatgtacca
      421 gatgggccaa ttccgcgatt atcaccacgg ctacatggtc cccagcaccg gcttcttgaa
      481 ctggttcgcc ctcagtcctg ccaatgaagg cttcacccta cagttccccc ggcgggagac
      541 cccgaccgaa gtgctgccgg cgcccaacta cccgaaactg ctggcgacct tcgagcaata
      601 cagcgactgg atcgagaccc tgggggtctc cagcgtcggt gcgctcaatg acgccatcca
      661 acaaggccgg acccgggagc tggtcttggt atcggaggcg ctgcacgaac accggatcgg
      721 cgagatggcg gcccagattc acgctggggg ggaagccgcc cggctggtgc tgatcgcggg
      781 accctctgcc tcgggcaaga ccactttctc caagcggctc tcgatccagc tcctggcgct
      841 ggggatctcg ccgctcccga tcgggcttga taatttcttc gtagatcggg aaatgacccc
      901 ccgcgacgag cagggagatt acgattacga gcgcctggat gccctcgatc tggaccggct
      961 gaatcacgac ctcggtcaat tgattgcggg acaacgggta gagctcccgc actatgactt
     1021 cgtcgcaggt cgccagatgg caggcgaggt cgtccagatc tcgaaggccc acatcatcat
     1081 cctggaaggg attcacggat tgaacccggc tctgcttccg gatatccccg gcgagagcgc
     1141 gattcgaatc tatgcttcgc cgctcaccca gctgaacctg gatcgctaca atcggatctc
     1201 gacgaccgac atccggcttg cccgccgcct ggtgcgggac gcctcccagc gcggaaagac
     1261 cgccgccgag accattgccc gttgggagtc ggtccgccgg ggggagaaac tgtatatttt
     1321 tccctatcaa gagaatgcgg acatgatgtt caactcggct ttggtgtacg aaggcgcggc
     1381 gctcgctcca ttggccgagc cactgctccg ccaggtcacc ttcgggacca acgaacacgt
     1441 cgaggccaaa cggctgctat cgtttctgga atggttcgtc ccggtcgaat catcccggat
     1501 tccagataac tcgatcctgc gggaattcat cggaggttcg atcctgcaag acttcaggat
     1561 ctggggggcc ggctagtttg cccgcgggcc cgctccggcg gggcattgtt tctgagttgc
     1621 caactccatg agcgagaccg cgatcaagct aatcggggtg ctgggggccg gcacgatggg
     1681 ggccggcatc gccctaaccg ccctttacgc cgggcattcg gtgatcctgg ctgaggtcgc
     1741 tccggaggcc ctgcagcgcg gccgggaata catcgagggc cacctggccc gcaaggggcg
     1801 gagcgagagc ctacggcagc tacagctcag caacgatctg caggcgctgg caggctccga
     1861 cgtagtgatc gaggccatca acgaagacct cgagctcaag cggaccgtgt tcgccgagct
     1921 cgaccggatc tgccctccac ccacgatcct ggccagcaac accagtaccc tgtcgattac
     1981 tgccatcgcg gctgccaccc ggacccctga acgagtggcc ggaatgcact tcttcaatcc
     2041 cgccccgcta atgcgcctgg tggaagtggc gcgcggcgcg gcgaccgctc aggccaccct
     2101 ggatgccctg atcgggctgg ccgagcggat gggcaagacc ccggtgctca ccctcgacac
     2161 tcccgggttc atcgtcaatc gcgtggcgcg tccgttctat ggcgaagcct tgcggctatt
     2221 ggcggagcag gttgccgggc atgaggcgat cgacctcctg gtgaccctcg gcggcggatt
     2281 tccgatgggg ccgttcgagc tgatggatct gattggactg gacgtcaatc ttcaagccgc
     2341 ccagtcgatg tacgactcca gctatggtga gccgcgctac cgtccgcaat cgatccaacg
     2401 ccggtatgtc gagagcgggc ggctcggccg gaagaccggg gtcgggttct accaataccc
     2461 aggcggcgag aagcagtgga aggcgcccga agttccggag cctcgccagc agtccggatt
     2521 cgtgctgatc tccgatggaa gctggggacc cgaggtgggc aggctgctag tccaggccgg
     2581 ctacaccctc agtgaaacgc atggagacct ccccctcgca gcgctgatcg tcaacggaag
     2641 ggaggaggga gtccgcgatg tgctccgtcg ctacgaccga gggctcgctc cggagatccc
     2701 aatcctgtgc cagaccgtcg acatcgctct cagcgaggtc gagcagagcg tccaacagcc
     2761 tcaacggctg attgggttcg atggtctgtt cttcgccgca gggcgggccg tcacgctggt
     2821 tgcacgcccg aatttgccgg acgccgctcg ccaggccgcg gagacactgg tccgatcgct
     2881 cggcaagctg ccggtgtggg tgagcgaaag ccctgcattg gtgctgccgc ggatcgtgtg
     2941 ctgcctggcg aatgaagccg cgttcgcggt cgccgagggg gtggccgagc cggaggcggt
     3001 ggacaccgcg atgaaattgg gggtcaacta tccgcagggt ccgctggaat gggccgccac
     3061 tctgggctac cggaaagtga ttgccgtgct ggaccacctg gcggacgagt acggggaaga
     3121 gcgctatcga gttgctccgt ggctgcgcaa ccgcgctcgc cgcagcctgt agtccataat
     3181 tgttggaatc gtggcggctt aagaaggaga ttgggagtga ctgatcgaag atttgtcggg
     3241 aagcccgcgg cagagttcga attcacggcc atcgagtacg aaaaggccga ctaccgagcg
     3301 acgatcacca tcaatcgtcc gaaggtgctc aacgcggtca atcatgccgt catagaagag
     3361 atgaacgtcg ccttgaagga cgccagctgg gacgactcgg tcgcggtgat cgtcctgacc
     3421 ggtgcaggcg atcgagcctt ctgcactgga gccgatctca gggagcagga agagttcctt
     3481 gaacggcccc gagattactg gaagtggatg ggggagttta tcgagctcca tgaacgactg
     3541 cgtaacctgg gcaagccgac cgtcgctcgg ctcaacggga tcgtcgtcgg gggtgggaac
     3601 gaattcaacc taagctgcga tctggcagtg gccgccgacg acatctacat tcggcaggtc
     3661 ggaaccagcc acggcagcgt ggccctggcc ggcgccaccc aattcctacc cttaattgtg
     3721 ggcgaccggc gggctcggga gattttgttg ttaaatgaag agatcccggc ggcgaaagcc
     3781 ctcgagtggg gattgatcaa ctacgcggta ccgcgggcgg agctggaccg ggcggtagac
     3841 gagttggttg ccaagctgat agccaaactc ccggagaaga cccgatacac caaacagcag
     3901 ctcaacttct ggcgcgacct gagctggcac ctgacgatcg ggcacgggcg ggaatggctg
     3961 gcgatccaca acaccagtcc ggagacctgg gaaggagtgc gggcgtttgg cgagaagcga
     4021 gctccggact acgattccat ccgccggcgg tgggcagagg atgattcacc cgaatggctc
     4081 ggcggccggc ctccgcggga tccccgctag tcgatgccag tatgatcgaa gccgaacgcc
     4141 tggaggcctt tgaggcccga attcaccggg gagagaagat tgaacccggc gactggatgc
     4201 cggatgagta tcgccgccag ttgatccgga tgatttcgca gcacgcccac agcgagatcg
     4261 tcgggatgct cccggaaggg gcctggatca cccgggcgcc caacctgcgc cgcaagctgg
     4321 tgctgctggc caaggtgcaa gatgaggccg gccacggtca atatctctac catgcggccg
     4381 agacgcttgg agcgacccgg gaagagatga tcgaagcgtt gctctccggc cgggccaaat
     4441 actcgagcat cttcaactac ccttctttgt cttgggccga catgggcgtg atcggctggt
     4501 ttgtggatgg ggccgccatc ctgaaccaga ccatgctcgc ccgctgctcc tatggccctt
     4561 actcccgggc caatatccgg atctccgccg aagagagctt ccatatgaag cagggcaagg
     4621 agcaggtcat tctgatgtcg caggggaccc ccctgcagcg gtcgatggtc caggatgccg
     4681 tggaccgctg gtggtggccg accatcatga tgttcgggcc ccacgaccac gattcgccca
     4741 acagcccggt cctcatccgc tggggaatca agaccaagac caacgaccag ctgcggcagg
     4801 attttgtgaa tcaggtggcc ccggaagtgc tggagcttgg gctgagcttg cctgacccgt
     4861 ccctccacta tgacgaggct tcaggaaact ggctcaccgg gtcgattgat tgggacgagt
     4921 tctggcgggt ggtcaagggc gatggaccgt gcaacgcgga gcggattgcg gcccgccgca
     4981 aggcgcatgc cgacggggag tgggtccggg cagccctggc ggcatacgcc gagcgtcagg
     5041 ccacggcatg agggataccc agtggccgcg cttccaggtg tttcaccagg agcacccgga
     5101 ccgaccgcat accaatgccg gagccgtgca cgcgcctgac gccgagctgg cgcttcagaa
     5161 cgcccgggat gtcttcgtcc gaaggccgga ctgtgtcagt ctgtgggtcg tccccgcgga
     5221 tcagattctc agcctcactg ctgaagagat cgagcacacg gatctgcccc cggcgccggc
     5281 agggaacggc gactcgcagc gctacctggt cttcatcaag cgcactcaag taggatctca
     5341 cgagcatgtg ggagcggtcg cggcggcgtc cggacccgcg gccttgggcg aggcggtacg
     5401 ccgctttggg acagcagatg cactggtttg gtgggttgtt tccgagagtc tgatcgagcg
     5461 gacggaggcc gccgaggtcg gcagtctgtt cgggccggcg aaggacaagc cctatcggga
     5521 ccaggccttc taccacacaa ccaccctaat gcggcagatt cgcgggcggc cgaaacaaga
     5581 accatgaaag ccgaattccg ggaatcgctt gcgaactatc tgaccgccct cggcgatgac
     5641 gaattgattc tggctcatcg cggctcggag tgggccggcc atgccccgat cctggaggag
     5701 gacatcgcct tcgccaattt agccttggac gagctgggcc atgcctcgac ctggtatcgg
     5761 ttggccgccg agctgcttaa tgaagacccg gagcgg
//
LOCUS       DATDPQ010000380         3076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2582447, whole genome shotgun sequence.
ACCESSION   DATDPQ010000380 DATDPQ010000000
VERSION     DATDPQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582447"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VI520_08500"
     CDS             complement(<1..722)
                     /locus_tag="VI520_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HLF37958.1"
                     /translation="MVETSTALAFPTELPSLTPTITLTPTPADTPTPSLTPTPTAIWG
                     DFPGPAELSATEIPPPMPEIAFGDNVLNVILLGSDARPDTGGFRTDTIMLVSVDPAAG
                     TAKLISIPRDLYVYIPGWRMDRINTADPRGGFEMMRQTILYNFGIDLDGWARTTFSGF
                     INAVDALGGITVQSTGYLTDECGGVYRSYGPGSYFMDGFEALCYARMRKATGDFDRLR
                     RQQEVLQAIFVKIVSIDGLSRLP"
     gene            complement(1119..2936)
                     /locus_tag="VI520_08505"
     CDS             complement(1119..2936)
                     /locus_tag="VI520_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37959.1"
                     /translation="MSFFADLEREDYDRSYRDRDLVARLAEYFRPQRRRVLIAALATG
                     LIAAGEAASPILVSQGVGLLQRNPAAVPASWIAGVLFLLGMLVWSVNWVRRRMLTRAV
                     GAVMLDLRTEAFAATAAHDLAFYDEHASGKIVSRITSDTNEFAQVAVLVTDVASEILQ
                     VAILIVVLLRIEWSLTLWVLGMIPIFLLVALGFRRLARRVIRRSMRGIANVNASIKEA
                     VTGITVAKNFRQEQAIYAEFDRINMQSYGANVRRGFVLSVVFPTLNAMMGFGTALMVF
                     LGGSSAIRGAVGVGTWYLFMRSLDRFWFPILSLSAFWSQIQGGLSAAERVFALIDTES
                     QVRQTDRLTIPRLHGQIRFEQVDFRYHSQEPVLEGFSLVIEPGESLALVGHTGAGKSS
                     IVKLITRFYEFQAGRLLVDGLDIRSLDLHDFRRQIGLVSQNPFLFAGTVIDNIRYARP
                     EVSDEEITALAQQIGSGEWLETLPQGLLSKVGERGGRLSMGQRQLVSLMRVLVQRPAI
                     FILDEATANIDPFTESQIQEALQLILAETTSILIAHRLSTVRACDRIVVLRQGRVIEQ
                     GDHDRLMAQGGHYAELYNTYFRHQSLAYVQQSRLAAAAS"
BASE COUNT          641 a          941 c          969 g          525 t
ORIGIN      
        1 ggcaatcgcg acaaaccatc gatactcaca atcttgacga agatcgcctg aagcacctcc
       61 tgctgccggc gcaagcgatc gaaatcgccg gtggctttgc gcatgcgggc gtagcagagg
      121 gcttcgaacc catccatgaa ataactgccc gggccgtagc tgcggtagac gcccccgcac
      181 tcatccgtga ggtagccggt cgattgcacg gtgatcccac cgagcgcgtc gaccgcgttg
      241 ataaacccgg agaaagtggt ccgagcccag ccgtccagat cgatcccgaa gttgtagagg
      301 atcgtctgcc gcatcatctc gaacccacct cgcgggtcgg cggtattgat ccggtccatc
      361 cgccagccgg gaatgtagac gtacaagtcg cgcggaattg aaatcagttt cgcggtgccg
      421 gcggctggat ccaccgatac cagcatgatg gtgtcggtcc ggaaccctcc ggtgtccggc
      481 cgcgcatcgc tccccagcag aatcacgtta agcacgttgt ctccgaacgc gatctccggc
      541 atgggtggtg ggatctcggt tgccgacagc tcagcgggtc ccggaaagtc gccccagatc
      601 gccgtcggag tcggggtaag ggagggagtc ggtgtgtcgg cgggagttgg agtcagggtg
      661 atggtcgggg tgagagaagg caactccgtc gggaaggcca gggcggttga cgtttcaacc
      721 acgcttgttt cgaggcccgc cccctgcacc gtatcttgca aaaggtcctg aagcgatgca
      781 aggcaagcga cagacagcgc tgcgggcaac ccaaggaaga gcagcaggcg agttttcaaa
      841 caagcgtcct tttgggcaac caaagaatca gacgcattct ggcggccaca agatcccgcg
      901 gattctattc caaactgaga ccccggccat ggatgcaatc catgtgccta cggaaggccg
      961 gttaaatggc agccgcccgc tttagccgga ggaagagggg tcgcgggcgg cttcgaggcc
     1021 tggacactct ggggggaagt gtccggctcc atagtaggtg attctacggt tggctcacct
     1081 tacagaggcc taacagagtc cggggggcca agccctggtc aactcgccgc agcagccagc
     1141 cgcgactgtt ggacataggc tagtgattgg tggcggaaat aggtgttgta taactcggcg
     1201 tagtggcccc cctgggccat caaacgatcg tggtcgcctt gctcgatgac tcggccctgc
     1261 cgcaggacga caatccgatc gcaggcccgg accgtggaca gccggtgggc gatcaggatt
     1321 gaggtggtct ccgccaggat cagctgcagg gcttcctgaa tctgggactc ggtgaaagga
     1381 tcgatgttgg cggtggcctc atccaggatg aagatcgccg gccgttgaac caagactcgc
     1441 atcagagaga ccagctgccg ctgccccatt gacaaccggc ccccgcgctc cccgactttg
     1501 ctgagcaacc cctgcgggag agtttccagc cattcaccgg agccgatttg ctgggcgagg
     1561 gcggtaatct cctcgtccga cacctccggc cgggcatagc ggatgttatc gatgaccgtc
     1621 ccggcgaaga ggaacggatt ctgagacacc agcccaattt gtcgccgaaa gtcatgcaaa
     1681 tcgagtgacc gtatatccag tccgtctacc agcaatcgcc ccgcctgaaa ctcgtagaac
     1741 cttgtgatca atttcacaat agaagacttg ccggcgccgg tgtgcccgac caacgccagg
     1801 ctttcgccag gctcaatcac caggctgaaa ccctcgagga ccggctcctg ggaatggtag
     1861 cgaaagtcga cctgctcgaa gcggatctga ccgtgcagcc tggggatcgt cagccgatcg
     1921 gtctggcgga cctgagattc ggtgtcgatc agcgcgaaca cccgctcggc agccgagagg
     1981 cctccttgga tttggctcca gaatgcagag aggctgagga tcgggaacca gaagcggtcc
     2041 aggctgcgca tgaacaggta ccaggtacca acccccacgg ccccgcgaat tgcggagctg
     2101 ccgccgagga acaccatcag ggccgtgccg aatcccatca tggcgttcag ggtcgggaac
     2161 acgaccgaca gcacaaaccc gcggcgaacg ttggcaccgt acgactgcat attgattcgg
     2221 tcgaactcgg cgtagatggc ctgctcctgg cgaaaattct tggcgaccgt gatcccagtg
     2281 accgcttcct taatgctggc gttgacgttg gcgatgccgc gcatcgagcg gcggatcaca
     2341 cgccgggcca ggcggcgaaa gcccagggcg accaacagga aaatcggaat catgccgagc
     2401 acccagaggg tcaagctcca ttcgatccgg agcaggacca cgatcagaat cgccacctgc
     2461 aggatctcac tggcgacgtc ggtgaccagc accgcgacct gcgcaaactc attggtgtcg
     2521 gaggtgatcc ggctgacgat cttccccgag gcatgctcat cgtaaaaggc caggtcgtga
     2581 gcggcggtgg ctgcgaaggc ttcggtccgc agatcgagca tcacggctcc gaccgcccgg
     2641 gtgagcatcc gccgccggac ccagttcacg gaccagacca gcatgcctag caggaagagc
     2701 acccctgcga tccagctggc ggggaccgcg gccgggttgc gctgaagcag cccgacccct
     2761 tgggaaacca ggatcggcga ggcggcctca ccggcggcga tcaacccggt cgccagcgcc
     2821 gcgatcagca cccggcgccg ctgtggacgg aagtactccg ccagccgggc caccagatcc
     2881 cggtctcggt agctccggtc gtagtcttcc cgctcgagat ccgcgaagaa tgacatcagt
     2941 cggacagccg ctggaagatc tggcggtacg ccggagatct ggccataagc tcatcatgag
     3001 tcccgagggc ggccattcgt ccgtttcgca gtaccagaat ctgatcggcc caacggattt
     3061 gtgagagccg gtgggt
//
LOCUS       DATDPQ010000381         3740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1806081, whole genome shotgun sequence.
ACCESSION   DATDPQ010000381 DATDPQ010000000
VERSION     DATDPQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806081"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VI520_08510"
     CDS             complement(<1..411)
                     /locus_tag="VI520_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLF37960.1"
                     /translation="MNSLLKKLKLEAVNAGACLGPQGWIRDPNGRQLVSYNPTTSEPI
                     ASVIQATSTTYDRVAQSAVDSFKAWREQPAPKRGLLIRDLAGLVREYKEPLGDLISLE
                     MGKIRAEGHGEVQEMIDVCDFAVGLSRQLYGLTMQ"
     gene            complement(484..1266)
                     /locus_tag="VI520_08515"
     CDS             complement(484..1266)
                     /locus_tag="VI520_08515"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HLF37961.1"
                     /translation="MIFLKLGGSLITDKARRETPKVAVLQRVAHEIHEARRIKPELKL
                     LIGHGSGSYGHPAAELAGIQSGVRTAEDWRGFLDTWAAAQRLHRLVLDTLSEAELPVF
                     SFLPSALAVANDGEIVAFPDEPIRKALRAGLIPLVQGDVAFDRVRGGVVLSTERIMAY
                     LAIRLKPERILLAGLEEGVYERYPERNRLLPELAPKDLPGIDLGGSEAPDVTGGMSHK
                     VQLAIELAQTIPGVEVRIFSGGMSGNVQAALLGYALGTRVRG"
     gene            complement(1263..2201)
                     /gene="mvk"
                     /locus_tag="VI520_08520"
     CDS             complement(1263..2201)
                     /gene="mvk"
                     /locus_tag="VI520_08520"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HLF37962.1"
                     /translation="MTERTAPGKVILFGEHAVVYGKPAVAVPVHSTRARAQVVDLPDD
                     PAGWVRLEAPGTQLSSWLHEMPEANPLAAAVRLSLEDSGQKLESALRITIESEIPVGA
                     GMGSSAAVSVAVLRAVAAHLGQELSAERVSDLAFNVEKLQHGTPSGIDNSVVAHERPL
                     YFISGQPPQFLQVGRPFTLMIGDSGDRLPTRESVAAVRERRSADPQSFDRLFAEIAAI
                     SEQGRRLIESGDTHQLGPLLDRNQTLLKAIGVSGKGLEQLIRAAKRAGALGAKLSGAG
                     RGGIMIALVEPASAEAVGIALREAGATRTIQTEVAA"
     gene            complement(2277..3149)
                     /locus_tag="VI520_08525"
     CDS             complement(2277..3149)
                     /locus_tag="VI520_08525"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HLF37963.1"
                     /translation="MLKALLRAMRPRQWAKNVFVFAALVFDEQLLRPEPLVRIMVGFA
                     MLCLISSSVYLLNDIADVEADRRHPVKRTRPIAAGELAIPVAAAAAGLLAAISLLISF
                     ALGNSFGWIVAGYFGLNLLYSLWLKHAAIIDVLIIAAGFVLRVAAGVSLIQVSRFSPW
                     LYVCTTLLALIIGFGKRRAELLLLADGANSHRPVLDGYNVELLDQLIVVVSATTIMAY
                     SLYTFSAENLPANHLMMLTIPFVLYGIFRYLRLIHVENIGGAPEELILTDRPLLINLL
                     LWGLFAIVILYVGQ"
     gene            3232..>3740
                     /locus_tag="VI520_08530"
     CDS             3232..>3740
                     /locus_tag="VI520_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HLF37964.1"
                     /translation="MAEPKERLDQLLVSRGLVESRALAQRMIMAGQVRLEGQLVLQPS
                     TRVAPDAAVEMISAPRFVSRGGEKLEAALQQFRLQPAGWVCADVGASTGGFTDCLLQH
                     GADRVYAIDVGHGQLHWRLRNDPRVVLMERKNARTLSELPEPIRLATVDVSFISLRLI
                     LPSVAGWLVA"
BASE COUNT          680 a         1216 c         1209 g          635 t
ORIGIN      
        1 ctgcattgtc aatccgtaga gctggcggga taggccgacc gcgaaatcac agacgtcgat
       61 catctcctga acttcgccgt ggccttcggc ccggatcttc cccatctcca gactgatgag
      121 atcgcccagg ggttccttgt actcccggac caggccggcc aggtcccgaa tcagcagccc
      181 gcgtttgggt gccggctgct ccctccaggc cttgaagctg tccaccgccg actgggcgac
      241 ccggtcatag gtggtcgagg tcgcctggat cacggaggcg atcggctcac tggttgtggg
      301 gttgtaggac acgagctggc ggccgttcgg gtcgcggatc cacccctgcg ggcccaggca
      361 ggccccggcg ttcaccgctt cgagcttgag cttcttcaat aatgaattca tccatcgccc
      421 tccaggccgc aaacagatcg cggccagttt agcatggcga accggaccgt tgcccggagg
      481 ccgctatccc cgcacccggg tgcccagcgc gtacccaagc agcgcagcct gcacgttgcc
      541 ggacatgccg cctgagaaaa tcctaacctc gactccggga attgtttgag ccaactcgat
      601 cgccaattga accttgtggc tcatacctcc ggtgacgtcg ggtgcctccg aaccgcccag
      661 gtcaattcct ggcaggtctt tcggcgcgag ctctggcagc agtcggttcc tttccgggta
      721 ccgctcatac accccctcct ccagcccggc gagcaagatc cgctctggct tgagccggat
      781 ggcgagatag gccatgatcc gctcggttga caggactacc ccaccgcgga cccggtcaaa
      841 ggccacatca ccctggacca agggaatcag gccggcccgg agtgccttgc ggatcggctc
      901 atccggaaag gcgacgatct cgccatcgtt ggccaccgcc agggcggagg ggaggaatga
      961 gaacaccggc agctcggcct cgctcagggt atccagcacg agtcggtgga gccgctgcgc
     1021 cgcggcccag gtgtccagga aaccccgcca atcctcagcg gtccttacgc cactttggat
     1081 cccggcgagt tccgcggccg ggtgtccgta cgagcccgag ccatgcccga tcagcagctt
     1141 cagctccggc ttgatccgcc tggcctcatg gatttcgtgc gccacgcgct gcaagacggc
     1201 aaccttcgga gtctcgcggc gggccttgtc ggtgatcagt gatccgccaa gcttgaggaa
     1261 gatcatgccg caacctcagt ctggatcgta cgggtcgctc cggcctctcg cagcgcgatc
     1321 ccgaccgcct cggccgacgc cggctccacc agcgcgatca tgatcccgcc ccggcccgcc
     1381 ccggacaatt tagcgcccag cgctccggcc cgtttggcag cccggatcag ctgttcgagg
     1441 cccttgccgc tgaccccgat cgccttcagc agggtctgat tgcgatccag gagcggtccg
     1501 agctgatggg tgtcgcccga ttcaatgagg cggcggccct gctcgctgat ggcagcaatc
     1561 tcggcaaaca gccggtcgaa actctgcgga tcggctgacc ggcgctcgcg aacggctgcg
     1621 actgattctc gggtggggag gcggtcgccg ctgtcgccaa tcataagcgt gaacggacgc
     1681 ccgacctgca ggaactgcgg cggttggccg gagatgaagt acagcgggcg ttcgtgggcg
     1741 acgaccgaat tgtcaatccc ggagggggtc ccatgctgca gcttctcaac gttgaaggcc
     1801 aggtccgaaa cccgctccgc ggacagttcc tgccccaggt gggccgcaac cgctcgtaga
     1861 actgccacgc tcactgcggc gctggacccc atcccggcgc caactgggat ctcggattcg
     1921 atcgtgatcc gcagggcact ttcgagcttt tgacccgaat cctctaagct gagccgcact
     1981 gcggccgcaa ggggattcgc ttcgggcatt tcgtgtagcc atgagctaag ctgggtcccc
     2041 ggagcttcca agcggaccca tccggctgga tcatcgggta ggtcgacgac ttgggcccgg
     2101 gcccgggtgg agtgaaccgg cacggccacg gccggcttgc cgtagacgac ggcatgttcc
     2161 ccgaagagga tgaccttccc gggtgcggtt cgctcggtca tgctcgatcc gtcaggggtc
     2221 gcgggtccgg acggaggacc cggatcacgg cgtgcggtgg ctgcttgggg agatgctcac
     2281 tggccgacgt agaggatgac gatcgcgaac aagccccaca agagcagatt gatcaagagc
     2341 gggcggtcgg tgaggatgag ttcctccggc gcgccaccga tgttttcgac atggatcaag
     2401 cgaagatagc gaaagatccc atacagcacg aacgggatgg tcagcatcat gagatggttg
     2461 gccggcaggt tctcggcaga aaaagtgtag agcgagtatg ccataatggt cgtcgccgag
     2521 acgaccacga tcagctgatc cagcagctca acgttgtacc cgtccagcac tgggcggtgt
     2581 gagttggcgc catcggccag cagcagcagt tcggcccgcc gcttgccaaa tccaatgatc
     2641 agggccaaca acgtcgtgca cacgtacaac cagggggaaa atcgcgagac ctggataagg
     2701 ctgacccccg ccgcgaccct gagtacaaac ccggctgcga tgatcagcac atcgatgatc
     2761 gcggcgtgct tgagccacaa cgagtagagc aggttcaacc cgaagtaacc ggctacgatc
     2821 cagccgaatg agtttccgag ggcaaaactg atcaggaggg aaatcgcggc gagcagcccg
     2881 gcggccgcag ccgctaccgg gatagccaat tccccggcgg cgatcggccg ggtgcgcttg
     2941 accggatggc ggcggtcggc ctcgacgtcg gcgatgtcgt tgagcagata gaccgagctc
     3001 gagatgaggc acagcatcgc gaacccgacc atgatgcgta ccagcggctc cggccggagg
     3061 agctgttcgt cgaagaccag cgcggcgaac acaaatacgt tcttggccca ctgtcgcggc
     3121 cgcatggctc gcaggagcgc cttgagcatg ccgcgaagga tagcagttgc aaccgcggcg
     3181 agcaagcgga cggccctccc atgccgcgag agttcaggct cggtcgaata gatggcggaa
     3241 ccgaaggaac ggctcgatca actgctcgtc tcgcgcggcc tggtcgagag ccgcgccctc
     3301 gcccagcgga tgatcatggc cggccaggtg cggctggaag gacagctcgt gctgcagccc
     3361 tcgacccggg tggccccgga cgcggcggtc gaaatgatct ccgccccccg cttcgtctcc
     3421 cggggtggtg agaagctgga ggcggccctc cagcaattcc ggctgcagcc ggccggatgg
     3481 gtgtgcgccg acgtcggggc ctcgaccggc ggattcaccg actgcctgct gcagcacggc
     3541 gccgaccggg tttatgcgat cgatgtcggc cacgggcagc tgcactggcg gctgcggaac
     3601 gatcctcggg tggtgctcat ggaacgcaag aacgccagaa ccttgagcga actccccgag
     3661 ccgattcggc tggcgacggt cgatgtgtct ttcatctcgc tgcgcctcat cctgccttcg
     3721 gtggcgggct ggctggtcgc
//
LOCUS       DATDPQ010000382         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2861096, whole genome shotgun sequence.
ACCESSION   DATDPQ010000382 DATDPQ010000000
VERSION     DATDPQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2861096"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1421)
                     /locus_tag="VI520_08535"
     CDS             complement(174..1421)
                     /locus_tag="VI520_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLF37965.1"
                     /translation="MGPVAMRLTLINQFYPPDFAPTGELAASLAEHRVQLGDQVTVVT
                     SQGGYVAESPVEAAPPASNPRIHRLWTPRLGKQTLALRLIDYSFFYAQAAIRLALLPR
                     QDLIVSLTTPPLIALAGALHKWLHRETRLILWSMDCYPEVPERAGVLRPGSLPNRLIR
                     WLNRRLFRALDYLVCLDTPMEQLLKSQHESANPKLPTSVIHNWEPALSYPIDLRPPPW
                     PMAKELDLSGKFVVLYLGNAGYGHQFETVLDAAEQLRSEPVAFIFVGGGQKWSELRRA
                     KEQRGLASLHVLRYLPRPETPSVMAAADCALVTLRDSYLGVISPSKIHSNLAMGLPLI
                     YIGPEGGNVDEAIQRYQCGVSLRHGDVNGMVEFIRRLQAQPERSKAFRSQARRAFEEA
                     YSDVVCLPQFDRVLEQLVGRAPA"
     gene            1493..>2080
                     /locus_tag="VI520_08540"
     CDS             1493..>2080
                     /locus_tag="VI520_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37966.1"
                     /translation="MPEAKSIPPFSQQAPWPVQLYSALLSHWPRVFALAVLGGLLGLG
                     ASWVSPARYLATARISVGIDYGRAQFADEDAERHILARVQDLLLSDEALERALDSFVL
                     AGEAAPSLGGFREQVRLTRIEAEWSLGVTDPSPGRAAGLASTWAAASLGLLEESQQEA
                     WKLAELQALFFQFACRPPAPGESGDPPVWLCDELQG"
BASE COUNT          372 a          644 c          671 g          393 t
ORIGIN      
        1 gccgcccgca gctggcccga aatcatgcgg gttcactttc gagatggggg gagcgtgttc
       61 tccattatct atgccaagta cctgctgcgg cggctgctgg agccggtgat gccctggcgg
      121 gtgaaactgc gcttgtttcg ctacggcctg gagcagaagg tccgctgggg tgactaagcc
      181 ggggcgcggc ccacgagctg ttccaacacc cggtcaaatt gcggcaggca cacaacgtct
      241 gaataggcct cctcgaaagc gcggcgggcc tggctcctga aagcttttga ccgctccggt
      301 tgtgcctgca gcctccggat gaactcaacc attccgttca cgtccccgtg acgtaggctg
      361 accccgcact gatagcgctg gatggcctca tccacgttgc ctccctctgg cccgatataa
      421 atcaagggca gccccatggc caggttggag tgaattttgc tcgggctgat cacgccgagg
      481 tacgaatcgc gcagcgtcac caatgcgcag tcggccgccg ccatcacgga cggcgtttcg
      541 ggtcttggta ggtagcgcag cacgtgcagg ctggccagcc cgcgctgttc cttggctcgc
      601 cgcagctcgc tccatttctg accgcctccg acgaagatga aggcgacggg ttcgcttcgc
      661 agttgctcgg cagcatccag caccgtttca aactggtgcc catagccggc gttcccgagg
      721 tagagcacca cgaacttccc gctgaggtcg agttctttgg ccatcggcca tggaggcggt
      781 cgcagatcta tgggatagct cagtgcgggt tcccaattgt gaattaccga ggttggcagt
      841 ttggggttgg cactctcgtg ctgagacttc agcagctgct ccataggagt atccaggcag
      901 accagataat ccagggcccg aaagagccgc cggttgagcc agcggataag ccggttcgga
      961 aggcttcccg gacggagcac cccggcccgc tccggcacct ctgggtagca gtccatgctc
     1021 cacaaaatga gccgagtctc ccgatgcagc cacttgtgca gtgcgccggc caatgcgatc
     1081 aggggcgggg tcgtcagcga aacgatcagg tcttgccgcg gtagcagagc cagccggatc
     1141 gcggcttggg cgtagaaaaa gctgtagtcg atcagccgca gggcgagggt ctgctttccc
     1201 aaccgggggg tccacagccg atggatccgc gggtttgaag cgggaggggc ggcctcgacc
     1261 ggcgactcgg caacatagcc tccctgactg gtgaccaccg tcacctgatc gccgagttgg
     1321 acccggtgtt cggccaacga ggcggccagc tcacctgtgg gcgcgaaatc ggggggataa
     1381 aactgattga tcagggtcag gcgcatggct accggcccca ttgtatctag agagccttgc
     1441 ccaagcccaa gcgcctcggt tggagcagca gtgttagact agcaaaccgc ctgtgcccga
     1501 agccaagtcc attccacctt tcagccagca ggctccatgg cccgtccagc tctactcggc
     1561 actccttagc cactggcccc gggtcttcgc cctggcagtc ctgggagggt tgttgggact
     1621 gggcgcgagt tgggtcagcc cggctcgcta ccttgccacg gcaagaatct cggtcgggat
     1681 tgattacggc cgagctcagt tcgcggacga ggacgccgaa cgccacattc tggcgagagt
     1741 tcaggacttg ctgctgtcgg atgaggccct cgagcgggca ttggactcat tcgtgctcgc
     1801 cggtgaggca gcgccaagcc taggcggctt ccgagagcaa gttcggctga cgcggatcga
     1861 ggccgaatgg tcactcgggg taaccgatcc cagccctggc cgggccgcgg ggctggccag
     1921 cacctgggct gcggcgtccc tcggcttgct tgaagaatcc caacaggagg cctggaagct
     1981 ggctgagctg caagccctgt tctttcagtt cgcttgtcga cccccggcgc caggcgaatc
     2041 tggagatcca cccgtgtggc tgtgcgacga actccaggga
//
LOCUS       DATDPQ010000383         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2868745, whole genome shotgun sequence.
ACCESSION   DATDPQ010000383 DATDPQ010000000
VERSION     DATDPQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868745"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VI520_08545"
     CDS             <1..939
                     /locus_tag="VI520_08545"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HLF37967.1"
                     /translation="EAINLVAHSWGRANLKAGDVVLLTEMEHHSNIVPWLMLAKEIGF
                     EIHYVSFGTDYQLDQQQYRRLLETRPKLVGFTMMSNVLGTITPVGEMTAAAHAAGAVV
                     LVDGAQAVPHLQLNVAQLGVDFLAFSAHKMCGPSGIGALYGRRELLERMPPFLGGGEM
                     IKRVRLDSFEPNEVPHKFEAGTPPIAEAVGFGAAVDFLTELGMPAVMEHERMMVTRAV
                     EQLEEVPGVSVYGPTPEHKGGVAAFTFETAHAHDVAQLLDREGIAVRAGHHCAMPLHE
                     KLGLPATTRASFYLYNTPEEIDRLIEGLYKVREVFA"
     gene            963..1361
                     /locus_tag="VI520_08550"
     CDS             963..1361
                     /locus_tag="VI520_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HLF37968.1"
                     /translation="MDDLYREQIIDRYKNPRMRGKLDPHDYSYEDDNPLCGDHVRIDL
                     RVDGEGRVSEAAFSGEGCAISQASADLLMESVVGKPLEEVKGYGKEALLEMLGIELGP
                     VRMKCALLSLKVLKAGVYGLEHLEEELAAI"
     gene            1427..1777
                     /locus_tag="VI520_08555"
     CDS             1427..1777
                     /locus_tag="VI520_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HLF37969.1"
                     /translation="MYNYSLVNDEKLEFVTVATVGELGNGDRLILEVGGETIAVFNIA
                     GQYFAISDVCSHDDGPVAEGELREYQIECPRHGAHFDVRSGKVLSLPAVVDIPAYPVR
                     VVGDEIQVGLPAEG"
     gene            1865..>2453
                     /locus_tag="VI520_08560"
     CDS             1865..>2453
                     /locus_tag="VI520_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HLF37970.1"
                     /translation="MAKKWVYAYDEVEEAEGVAGSWDGVKALLGGKGANLAEMTRLEI
                     PVPEGFVITTEACNTYLAAGEQFPEGMWDQVLAALATIEKRTGKRLGDPADPLLVSCR
                     SGAKFSMPGMMDTVLNIGLNDQTAAGMVKLTGDPRFVYDSYRRLVQMYGSVVMGVADE
                     PFEHVLTEFRKQRGVESDSDLTAEDWKKITERFKEI"
BASE COUNT          463 a          701 c          832 g          457 t
ORIGIN      
        1 gaggccatca acctggtcgc ccatagttgg ggtcgcgcca atctgaaggc cggcgacgtt
       61 gtcctgctga ccgaaatgga gcaccactcc aacattgtcc cctggctgat gctggccaag
      121 gagatcggct tcgagatcca ctacgtttcc ttcggcacgg attatcagct cgatcagcag
      181 cagtatcgca ggctgctgga gacccgccct aaactggtcg gattcaccat gatgtccaac
      241 gtgttgggga cgatcacccc cgtcggcgaa atgaccgcgg ccgcccacgc ggccggcgcg
      301 gtggtgctgg tggacggggc gcaggcggtg ccgcatctcc agctaaacgt cgctcagctc
      361 ggagtggatt tcctggcctt ctctgcccac aagatgtgcg ggccgtccgg gatcggggcc
      421 ctctatgggc ggcgcgagct gctcgagcgg atgccgccct tcctgggggg tggcgagatg
      481 atcaagcggg tgcggctcga ctcattcgaa ccgaatgagg ttccccacaa gttcgaggcc
      541 ggcaccccac cgattgccga ggcagtcggc ttcggtgcgg cggtggactt cctgaccgag
      601 ctcggcatgc cggcggtgat ggagcatgag cggatgatgg tcacccgggc cgtcgaacag
      661 ctggaggaag tgcccggagt cagcgtctac ggcccaaccc ccgaacacaa gggtggggtc
      721 gcagccttca ccttcgagac cgcccatgcc cacgatgttg cccaattgct cgaccgggaa
      781 ggaatcgcgg tacgggccgg ccatcactgt gccatgccgc tgcacgagaa gctcggcctg
      841 ccggccacca cccgggcttc cttctatctg tacaacaccc ccgaggagat tgacaggctg
      901 attgagggcc tgtataaggt gagggaggtc ttcgcttagc acccgcgata cagcgaccga
      961 cgatggacga tctctaccgc gaacagatca ttgaccggta caagaacccc cgcatgcggg
     1021 ggaagctgga cccgcatgat tacagctatg aggacgacaa cccgctgtgc ggcgatcacg
     1081 ttcggatcga tctccgggta gatggcgagg gccgggtaag cgaggccgcg ttctccggcg
     1141 agggttgtgc gatttctcag gcctcggccg acctgttgat ggaatcggtg gttggcaagc
     1201 cgttggagga ggtcaagggg tacggcaagg aggctttgct cgagatgctt ggaatcgagc
     1261 tcgggccggt ccgcatgaag tgcgccctgc tgtcgctcaa ggtgctgaag gcgggagtat
     1321 acggcctgga gcacttggaa gaggaattgg cggcgatcta accgccagac cgcgcgcctc
     1381 gcttgcccgc cggccgcgtc cggcgttttt tctgtagcag acccacatgt acaactactc
     1441 cctggtaaat gacgagaaac tggaattcgt gacggtcgcg accgtaggcg agctcgggaa
     1501 tggggatcgc ctgatcctgg aggtcggcgg agaaacgatc gcggtattca acattgcggg
     1561 ccagtatttc gccatctcgg acgtgtgttc gcatgacgat gggccggtcg ccgaaggcga
     1621 gctgcgggaa tatcagatcg agtgtccccg gcacggggcc catttcgatg tgcgcagcgg
     1681 caaggtgctt tcgcttccgg ctgtggtaga tatcccggcc tatccggtgc gggtcgtggg
     1741 ggacgagatt caggtcggcc tgcccgcgga aggttaaagc cgcggcctgc agttttgcag
     1801 taacattgtt cccggcaccg cgcggcgagg attcgaacgc ggtgttctta tgagggtgat
     1861 ggttatggcg aagaaatggg tttacgcata cgacgaagtc gaagaggcgg aaggcgtcgc
     1921 cgggtcgtgg gacggagtga aggctctgct gggtggcaag ggcgccaacc tggccgagat
     1981 gacccggctc gagatcccgg tccccgaggg ttttgtgatc accaccgagg cctgcaacac
     2041 ctacctggcg gccggggagc aattcccgga ggggatgtgg gatcaggtcc tggcggccct
     2101 ggccacgatt gagaaacgga ccgggaagcg cctcggtgat ccagccgatc cgctgctggt
     2161 ctcctgtcgt tccggcgcca agttctcgat gcccgggatg atggacacgg tattgaacat
     2221 cgggctgaac gatcagaccg ccgccgggat ggtcaagctg accggcgatc cccgctttgt
     2281 ctacgattct taccggcgcc tggtccaaat gtacggctcg gtggtcatgg gcgtcgccga
     2341 cgaaccgttc gagcacgtgt tgaccgaatt tcgaaagcag cgaggggttg aaagcgactc
     2401 ggacctgacc gcggaggatt ggaagaagat taccgaacgg ttcaaggaaa tcg
//
LOCUS       DATDPQ010000384         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_243778, whole genome shotgun sequence.
ACCESSION   DATDPQ010000384 DATDPQ010000000
VERSION     DATDPQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243778"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VI520_08565"
     CDS             <1..907
                     /locus_tag="VI520_08565"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLF37971.1"
                     /translation="KTFNRMVEGLREGSIYRDLLGRTVTPEVRAQLRTAFSDGALILK
                     GQNAEATILFADFRGYTSMAENTDPALVMKTLNDYFSGVVPIISLHGGVVNKFDGDSV
                     MAFFGILPRYLPPRVSALQATHAGLAMLEHIHKVNRERESARQPSFQMGIGISTGTVI
                     AGGLGSEDRVHYTVVGDTVNTAQRIQEASQTLGGTALVIAESTYRKLGAVRSQFQFGQ
                     TGRAALKGKQREVMVYQVLGRKSRLVEQAQLEQQVKQYTASLPRITQILSGQPTDSNE
                     QPEVSPEERDFLKERLPPSEGVEEL"
     gene            1001..1939
                     /locus_tag="VI520_08570"
     CDS             1001..1939
                     /locus_tag="VI520_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37972.1"
                     /translation="MDSRLARWLALTAFAVLIAGWFAAALHLTGASAGQPGFPFNLRS
                     NLAADYGADPGAGRITSLRISIIADWLKDLGFGEDDSELLAGLDSPVPTATARNFEGD
                     PPFTATMTPSPTQTNTPLPTSTSTPKPPTKTPKPTDTEVPEPTELPDDSIAPSISGGS
                     FFPTFGTLLPLCDGNDIAITGLRVEDPGPSSGFGLELPDNGWVKLKYEILGPGSTGYV
                     YSADLGPPLTGGWTDGPGSSWDAYYKGTLTIDFSDGYAFTAGGKLFFRPPLSGPTETP
                     TPEPFTVKVWSIVQDNAGNESYVLHGTYTLDSACGG"
     gene            1973..3010
                     /locus_tag="VI520_08575"
     CDS             1973..3010
                     /locus_tag="VI520_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37973.1"
                     /translation="MATNTERRTPFDLRTTIPLAKTTLLSLARKPKRSWLIPFLTLIT
                     GITFATMMLELMFSPGPLSALAFEGELRSKLIADYGADPLGAKIQGLRLTIMDEVLRG
                     GSDQGSGSAPRDPILSDPVPTATPAGQAEATATGETLATVEPTATGPSFTSTPLAAPV
                     ATASNTPIPIPLATNPPGSLCDKLSIVGIWIDGDDKLKTSVRNQASVKAFLTDTILDW
                     PDVPAPAYVDWFEFDGNRYYNGDDSSSPTSMTGSSRQLSAGKTRTWEADWDDEPAEGL
                     YGSFSVTLVFKVSGEFCTLGSSTFKSPPATPEPSPLPSETPLPPTETVLAPTATPPPT
                     ETPTETPTPVA"
BASE COUNT          618 a          939 c          932 g          548 t
ORIGIN      
        1 gaagaccttc aaccgaatgg tcgaaggctt gcgggaaggc tcgatctacc gagatttgct
       61 tggcaggaca gtgacccctg aggtgcgcgc ccagctgcgg accgccttct ccgatggggc
      121 gttgattctg aaaggccaga acgccgaggc gacgatcttg ttcgctgact tccgcggcta
      181 caccagcatg gccgagaaca cggatccggc gctggtcatg aagaccctga acgactactt
      241 ttcgggcgtg gtgccgatca tctcgctgca tggtggagtg gtcaacaagt ttgatggtga
      301 ctcggtgatg gccttctttg gcatcctgcc ccgatacctc cccccacgag tcagcgccct
      361 gcaggcgacc cacgccggcc tcgctatgct ggaacacatc cacaaagtca accgagagcg
      421 ggagagtgcg agacaaccct cctttcaaat ggggattggc atctcgaccg gcaccgtgat
      481 tgccggtggg ttgggcagcg aggaccgggt gcactacacg gtggtgggcg acacggtgaa
      541 cacggcacaa cgaattcaag aggcaagtca gacgttgggt gggaccgctc tggtgatcgc
      601 cgagtccacc tatcgcaagc tcggagctgt ccggagtcaa tttcagtttg gtcagacagg
      661 ccgagccgca ctgaagggca agcagcggga agtgatggtg taccaggtgc ttggccgcaa
      721 gagccgcttg gtggagcaag cccagctgga gcagcaggtc aagcagtaca ctgcgagcct
      781 accccggatc acccaaatcc tctcgggcca accgacagac tcgaacgagc agccggaggt
      841 cagcccggag gaacgggact ttctcaagga gcgtttgccg ccttccgaag gcgtggaaga
      901 gctgtaggaa ggacggcacc ttgtttcatc attggcatcc agcgaaacta agagccaggc
      961 gtggtcatgt aagggggtca gccttgcatc tactgccaga ttggactctc gactcgctcg
     1021 ctggctcgcg cttaccgcat tcgccgtcct catcgccggg tggttcgcgg cggctttgca
     1081 cctgaccgga gcttccgccg gccagcctgg gtttcccttc aatctacgtt ccaacttagc
     1141 ggcagactat ggagccgatc cgggggcggg caggatcact tcgctgcgga tctcgatcat
     1201 cgccgattgg ttgaaagatc ttgggtttgg ggaggatgat tctgaactgc tggctggact
     1261 ggactcgccg gtgcccaccg ccaccgcccg gaatttcgag ggcgacccgc ctttcacggc
     1321 gacgatgact cccagcccga cacaaaccaa cacgcccctg ccgacatcga cctcaactcc
     1381 aaagcctccg acaaagactc ccaagccaac cgataccgag gtcccagagc caactgaact
     1441 gccggacgac agcattgcgc cgagcatctc aggaggctca ttcttcccca ccttcgggac
     1501 gctgctgccg ttgtgcgacg ggaacgacat cgcgatcacc ggcctgcgcg tagaggatcc
     1561 aggcccgtcg agtgggtttg ggctcgaact gcctgacaac ggctgggtca agctcaagta
     1621 cgagatcctc gggccgggat cgactggata tgtatattct gcggatcttg gtccgccgct
     1681 cactggaggg tggacagacg gccccggctc gagttgggat gcctattaca agggcactct
     1741 cacgatcgat ttctcggacg ggtatgcgtt cacggccggt ggcaagctgt tcttccggcc
     1801 gccgctcagt ggtccgaccg agacgccgac accggaaccg ttcaccgtca aggtgtggtc
     1861 gatcgtccaa gacaacgccg gcaatgagag ctatgtgctg cacggaacgt acactctgga
     1921 cagcgcctgt ggcggttaga atgaatcagt tcacaagagg gatcggctga acttggcaac
     1981 caacacagag cggcgcaccc cgttcgatct aagaactacc atcccgctgg ccaagacgac
     2041 cctgctcagc ctggcccgca agccgaagcg aagctggttg atccccttcc ttactttaat
     2101 caccggtatc acatttgcaa cgatgatgtt ggagctgatg ttctcgccgg ggccgctcag
     2161 cgcgctggct ttcgagggcg aactgcgttc gaagctaatc gccgactacg gagctgatcc
     2221 gctgggggcc aagatccagg ggctgcggct gacgatcatg gatgaggtat tgcggggagg
     2281 cagcgatcag ggctccgggt cagcgccgag ggatccgatc ttgagcgacc cggtcccaac
     2341 cgccaccccg gccgggcagg ctgaggcgac cgcgacgggc gagacgctcg ccacggtcga
     2401 gccgaccgcg actggaccgt cgttcacgag cactccgctg gccgcgcccg tcgcaaccgc
     2461 gagtaacacg ccgatcccaa tcccgcttgc gaccaatccg cctggctcgc tatgcgacaa
     2521 gctctcgatc gtcggcatct ggatcgatgg cgatgacaaa ctcaaaacca gcgtgcgtaa
     2581 tcaggcctcg gtgaaggcgt tccttaccga tacgatcttg gactggccgg atgttccggc
     2641 gccagcctat gtggattggt tcgagtttga tggcaaccgg tactacaacg gggacgattc
     2701 cagttctccg acgagcatga ccgggtcgag ccgccagctg agtgctggga aaacgcgaac
     2761 ctgggaagcg gattgggacg acgagccggc ggaagggctc tacggaagtt tcagcgtgac
     2821 cctggttttc aaggtttccg gggaattctg caccctgggc agcagcacct tcaagtctcc
     2881 gccggcgacc ccggagccct cgccactgcc aagcgaaacg ccgcttccgc cgacggagac
     2941 tgtacttgcg ccgaccgcaa ctcccccgcc gaccgagact cctaccgaga ccccaacccc
     3001 cgtcgcctga gaggaagaaa ggcgagtccg aacagcg
//
LOCUS       DATDPQ010000385         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1036787, whole genome shotgun sequence.
ACCESSION   DATDPQ010000385 DATDPQ010000000
VERSION     DATDPQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036787"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VI520_08580"
     CDS             <1..468
                     /locus_tag="VI520_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLF37974.1"
                     /translation="VLDSAGAADMDHPAIAKYLYNDLGVPDWWSQMVTVGYEQARGRR
                     RVHETARGFQISRSRTMEVSAARAFKAWSEAAVRNRWLPGRDLKVRKATRNKSIRSTW
                     SADGSDVMVDFVAKGPGKVQVVVQHSRLSDPKQAERMKEFWSAALGRLKAQLE"
     gene            572..901
                     /locus_tag="VI520_08585"
     CDS             572..901
                     /locus_tag="VI520_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HLF37975.1"
                     /translation="MAYDKKLADRIRKALSKQPKLTEREMFGGIGFMLRGNMACGVIG
                     DEMMVRIAKEETDSAIKEPGARIFDFSGRPSPGWILVNQSVLTKPAEIRGWVKRGVEY
                     ALSLPAK"
     gene            926..1798
                     /locus_tag="VI520_08590"
     CDS             926..1798
                     /locus_tag="VI520_08590"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HLF37976.1"
                     /translation="MGDLDRALISLAGLSVGDAFGEQFFRLPWDAIRERRLPPGPWPW
                     TDDTAMALSIVEVLRNHHAIEQDVLAARFAERFRQDPNRGYGGGAVRLLSQVAEGASW
                     EVEAPRLFDGGSYGNGAAMRAGPIGGYFWGDPERAAQEAQRSAIVTHSHLEGQAGAIA
                     VAAAAAMAPFKLAISSLDFLSQVLNRLPPTRTREKIEEARRIGPDEHRRAVTTLGTGY
                     EIAAFDTVPFCLWVAAHHGFDFETALWTTIAGGGDRDTTCAIVGSIVGLSADIPETWM
                     RAREPLPAEFELGR"
     gene            1901..2485
                     /gene="msrA"
                     /locus_tag="VI520_08595"
     CDS             1901..2485
                     /gene="msrA"
                     /locus_tag="VI520_08595"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HLF37977.1"
                     /translation="MASHETPAATKNSEIATLGGGCFWCLEPVFEALSGVRTVTVGYA
                     GGELAAPTYRQVGSGTTGHAEVVQIEFNPRVISYREILEVFFSVHDPTTPNRQGADIG
                     TQYRSLVLSHSPEQQKTAEALVRELQAEGSWDSPIVTQVQPFRSFYLGEDYHQDYYEN
                     NSSAGYCRIVIDPKLAKFRKRYADKLKREPSPAG"
     gene            2494..>2714
                     /locus_tag="VI520_08600"
     CDS             2494..>2714
                     /locus_tag="VI520_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLF37978.1"
                     /translation="MSHDELVRRNKAHTLTSWTAQDDWNPLSMVKAEGVYFWDADGKR
                     YLDWSSQLFNVNIGHANPHVIQAIQEQVAR"
BASE COUNT          539 a          847 c          856 g          472 t
ORIGIN      
        1 gtgctcgact cggcgggagc ggccgacatg gaccatccgg ccatcgcaaa atacctttac
       61 aacgacctcg gcgttcccga ttggtggtcg caaatggtga cggtaggcta cgagcaagcc
      121 cggggacgca ggcgggttca cgagactgct cgaggctttc agatcagccg gtccaggacc
      181 atggaagttt cggctgcccg cgccttcaaa gcctggagtg aggcagcggt ccgcaaccgc
      241 tggctgccag gtcgggatct caaagtccga aaggcgaccc gcaacaaatc gatccggagt
      301 acctggtcgg ctgatggatc agatgtaatg gtggatttcg ttgcgaaggg ccccggcaaa
      361 gttcaggtgg tcgttcagca ttccaggctc tcggatccga aacaagccga acggatgaaa
      421 gagttctggt cggcggccct cggccggttg aaagctcaac tcgaatgacc gcatcaacag
      481 cgcgcgccga ctatgaggcg ccgacccaca tcgtgcgacc ccagcggcgc gactagcaag
      541 ctcccaatca tagaccggct tggatttgcc aatggcttat gacaagaagc tcgcagatcg
      601 aattcgaaag gcgctcagca agcagccaaa acttaccgag cgggagatgt ttggggggat
      661 cggcttcatg cttcgaggca acatggcctg cggtgtcatc ggcgatgaaa tgatggtgcg
      721 catagccaag gaggagaccg attccgcaat caaggagcct ggagcccgga tctttgactt
      781 ctcgggccgg ccctcgccgg gctggatcct ggtgaatcag agtgtgctta ccaagccagc
      841 cgagataagg ggctgggtca aacgaggcgt cgagtacgcg ctgagcctac ctgcaaaatg
      901 agccgctggg agtgagccgg cgcggatggg ggacctggac cgggcactca tctcacttgc
      961 cggcctctcg gtcggggatg cgtttgggga gcagttcttc cggctgccct gggatgcgat
     1021 ccgggagcgc cggctgccgc ccggcccttg gccctggacc gacgacaccg cgatggccct
     1081 ctcgatcgtg gaagtattgc ggaatcacca cgccatcgag caggatgtcc tggccgcccg
     1141 cttcgccgag cgctttcgcc aggatcctaa ccgtgggtat gggggcgggg ccgtccgact
     1201 gctttcgcaa gttgctgagg gcgcgagctg ggaggtcgaa gccccacgcc tgttcgacgg
     1261 cggatcgtac ggaaacggcg ccgcgatgcg ggccgggccg atcggcggct acttctgggg
     1321 cgatccggag cgggcggcac aggaagccca acgatccgca atcgtgaccc actcccacct
     1381 ggaaggccag gccggagcga tcgcggtcgc ggctgcggcc gcgatggcac cgttcaagct
     1441 ggccatctcg agcctcgact ttctatccca agttctcaat cgcctcccgc caacccggac
     1501 ccgtgaaaaa atcgaggagg cccgcagaat cgggccggat gagcacagga gggcggtcac
     1561 caccctcggg acaggttacg aaattgcggc ctttgacacg gtccccttct gcctctgggt
     1621 ggctgcccac cacgggttcg acttcgagac cgccctgtgg actacgatcg ccgggggcgg
     1681 cgaccgagac acgacctgcg cgatcgtcgg cagtatcgtc ggtctgtcgg ccgacattcc
     1741 ggagacctgg atgcgggctc gggagcccct cccggccgaa ttcgagctcg gccggtaatc
     1801 ctttcgcaag gcgggtcggg tagaatccgc ccgcgatcgc ggctgccctg caaccctact
     1861 tcgcaccgcg ctcgacactt tgaacggagg caaccgcacg atggcatccc acgaaactcc
     1921 agccgccacc aagaattcgg aaatcgcgac gctgggtggc gggtgttttt ggtgtcttga
     1981 accggtcttt gaggccctga gcggagttcg cacggtcacg gtcggatacg ccggcggtga
     2041 gctggccgca cccacttatc ggcaggttgg cagcggaacg accggccacg ccgaagtcgt
     2101 ccagatcgag ttcaatccgc gggtcatcag ctatcgagaa atcttggaag tcttcttctc
     2161 ggtccatgac cccacgaccc cgaatcgcca aggcgcggat atcgggaccc agtaccgctc
     2221 gctggtgctg tctcacagcc ccgagcagca gaagaccgct gaggcgctcg tccgtgaact
     2281 acaggctgaa gggagctggg attctccgat cgtgacccag gtccagccct tccgttcctt
     2341 ctacctgggc gaagactatc atcaggatta ctacgagaac aattcctctg ccgggtattg
     2401 ccgaatcgtg atcgatccga agctggccaa attccgcaaa cgctacgcgg acaagctcaa
     2461 gcgagagccc tccccggccg gctaaatccc ctcatgagtc acgacgagct cgtccggcgc
     2521 aacaaggctc acaccctgac cagctggacc gctcaagacg actggaaccc cctcagcatg
     2581 gtgaaggccg agggggtcta cttttgggac gccgacggca agcgttacct cgattggtcc
     2641 tcccagctct tcaacgtgaa catcgggcat gccaacccgc atgtgatcca ggcgatccaa
     2701 gaacaggtgg cgcg
//
LOCUS       DATDPQ010000386         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_2598129, whole genome shotgun sequence.
ACCESSION   DATDPQ010000386 DATDPQ010000000
VERSION     DATDPQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598129"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1252
                     /locus_tag="VI520_08605"
     CDS             17..1252
                     /locus_tag="VI520_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37979.1"
                     /translation="MSSPLSRLGFHYFPDDSHYTGRDLEQWLPVLLKLAARWLVLSAS
                     PKRSIPEPFVRGIVEAGIRPVVWIKSGMSDTSPRELAPLLTSYSAWGVQEVVVFDRPN
                     QRTSWTTTDWSRPDLVERFVDRMLPILQLEKSVGLNPVFPPLEPGGDYWDTAFLETAL
                     QSIQRRGQSALLRKMCLAIYGWTFGHPLDWGFGGPANWPEARPYEALEGIQDQIGFRT
                     FDWYGLITKSQIGIELPMIVVAGGPGDAEAARARPAQTVSEILRALQAAEITDSVLAF
                     NFYLLACDPDSQAYSNAWFAAPGEPKPLVAAAKRMIDASGKQVMLQVDKPLNHYVLLP
                     HAGLSDDSWMAARKLAQDAVVGFSTDEAAQAKRVTLVGDEAAIPPSVERRLIREGCLV
                     LRVGPSSTPSWDSAQRRRS"
     gene            1249..2385
                     /gene="ftsZ"
                     /locus_tag="VI520_08610"
     CDS             1249..2385
                     /gene="ftsZ"
                     /locus_tag="VI520_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLF37980.1"
                     /translation="MIPFTSDPQNMNAAPATAQRELAEFGQARIKVIGLGGGGCNAID
                     RMVELGLIGASFIAANTDCQALASSLAPTRVQLGPNVTRGLGSGGDPRVGAAAALESS
                     RELAQVFAGADMVFLTAGMGGGTGTGAAPIAAEIAREAGAVVIATVTMPFGFEMSRRS
                     KNAVEGVKRLQPHTHTLITVPNDRLLEAVPRNSSLDMAFRFADDVLRQGIQGIVELVT
                     KPGLINVDFAHVRSLMQQGGGALMAIGLGEGEAKASMAVHQALHHPLLEVNSLEFATG
                     ALLHFTGGDDLSLSEVGSAVESLRENLPLDVDVVLGVTPEPTMEGRVQVILVVTGIGG
                     RPVRSFVTDQPEPFSIVSDNLDVPAFLRKRSSGVPSAPRQPQAS"
     gene            2387..2620
                     /locus_tag="VI520_08615"
     CDS             2387..2620
                     /locus_tag="VI520_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37981.1"
                     /translation="MDRETVGKISSTVVGQFPEMDGVKPAIRRQAPPGDSPTLFLLTY
                     KAAARLPNGKSLQRVVRVVADDEGHVIRISTSR"
BASE COUNT          516 a          843 c          887 g          543 t
ORIGIN      
        1 aggctaagac ctggcaatga gctcacccct ttcgagacta gggtttcact acttccccga
       61 cgacagccac tataccgggc gggacctcga acagtggttg ccggtgctgc taaaactcgc
      121 cgcccgctgg ttggtcttgt ccgcctcgcc caaacggtcg atcccagagc cgtttgtgcg
      181 cgggatcgtg gaggccggaa tcagaccggt cgtctggatc aagagcggga tgtcggatac
      241 cagccctcgg gaacttgccc ctttgctgac cagctattcg gcctgggggg tccaggaagt
      301 cgtggtcttc gaccgaccca atcagcgcac cagctggaca accaccgatt ggagccgacc
      361 ggatttggtt gaacgatttg tggatcgaat gcttcccatc ctccaactgg agaaatcggt
      421 tggtttgaat ccggtcttcc ccccgcttga gccgggaggc gactattggg acaccgcctt
      481 tttggagacc gccctccagt ccattcagcg gcgcgggcag agcgccctac tgcgcaagat
      541 gtgtctcgcg atttacggtt ggaccttcgg tcatccgctc gactggggat ttgggggtcc
      601 ggctaattgg cccgaggctc gaccctacga agcgctggaa ggaattcaag accagattgg
      661 ttttcgaacc ttcgattggt acggacttat cacaaaatct cagatcggca tcgagctgcc
      721 catgatcgtt gtggccggtg ggcccggcga tgccgaggcg gcccgcgccc gcccggctca
      781 gacggttagc gagatcctac gcgccctgca agccgcggag atcacggatt cggtgctggc
      841 cttcaatttc taccttctgg catgcgatcc cgattcccaa gcctattcca atgcctggtt
      901 cgcggctccc ggcgagccaa agccgctcgt cgccgccgcc aagcggatga tcgacgcgtc
      961 cgggaagcag gtcatgcttc aggtcgacaa gcccttgaac cactatgtgc tcctacccca
     1021 tgcaggtctg tccgatgact cgtggatggc cgccaggaaa ttggctcaag atgcggtcgt
     1081 gggattctcg acagacgagg cagctcaagc caagcgggtc acgctggtcg gcgatgaggc
     1141 cgcaattccg ccttccgtcg agcgccggct gatccgcgag ggttgtctcg tgttgcgggt
     1201 cgggccttcg tcgacaccaa gctgggactc cgcgcaaagg agaaggtcat gatccctttc
     1261 acatcagatc cacagaacat gaatgctgct ccggccacgg ctcagaggga gctcgcggaa
     1321 tttggccagg cccggatcaa ggtcatcggt ctgggcggcg gcggctgcaa cgcgattgac
     1381 cggatggtcg aactgggctt gatcggcgcc agtttcatcg ccgccaatac cgattgccag
     1441 gcgctcgcct cgagcctggc ccccacccga gtccaactgg gaccgaatgt gacccgcggc
     1501 ctgggttccg gcggggaccc tcgagtggga gcggccgccg cgctggagag cagccgggag
     1561 ctggcgcagg ttttcgccgg ggcggatatg gtgtttctca ctgccggcat gggtggcggc
     1621 actggaaccg gcgccgcccc gatcgcggcc gagatcgccc gggaagccgg ggccgtggtc
     1681 atcgctacag tgacgatgcc ctttggcttc gaaatgagcc ggagatccaa gaacgcagtc
     1741 gaaggagtca agcggctgca gcctcacacc cacactctga ttacggtccc gaatgatcgt
     1801 ctgctggagg ccgttccccg gaattcgagt ctcgatatgg cgttccgctt cgccgacgac
     1861 gtgctgcggc agggaattca ggggatcgtc gagctggtca ccaaacccgg gttgatcaac
     1921 gtggattttg cccatgtgcg cagtctgatg cagcagggag gcggggcact catggcaatc
     1981 gggctcggcg agggcgaagc gaaagcctcc atggctgtac accaggctct gcatcacccg
     2041 ctgctggagg tcaacagcct cgagtttgct accggcgcat tgctccattt caccggagga
     2101 gacgatctga gtttgtcaga agtcggctcg gcggtcgaga gtcttcgaga gaatctgccg
     2161 cttgacgtgg atgtcgtgtt gggggtgacg ccggagccga ccatggaagg gcgggttcag
     2221 gttatcctgg tggttaccgg aatcggaggc cgtccggtcc ggagctttgt cacagatcag
     2281 cccgagccgt tctcaatagt ttcggacaat cttgacgtcc cggcctttct gcgtaaacgg
     2341 tccagcgggg taccaagcgc cccgcgccaa ccccaggcga gctagcgtgg atcgcgagac
     2401 ggtcggcaag atcagcagca cggtcgtggg gcagttccca gaaatggatg gtgtgaagcc
     2461 ggccattcga cgtcaagctc cccccggaga ctcgccgacc ctgttcctgc tcacctacaa
     2521 agcggcggct cggctgccga acggaaaatc cttgcagaga gttgtgcggg tcgtggccga
     2581 tgacgagggc cacgtgatcc gaatcagcac atcgaggtga acccatgaat aaactgaagt
     2641 atcgaccaat cgtggtgctc aatctgctac tgctcaagac caccgtcctg ttggcggagt
     2701 gggccctcca acaccgcgag gccgtgaggc tcagcaggac ctatgcaccg gtgtttctgg
     2761 gcggtttcgc ggcctacttc gccggccgg
//
LOCUS       DATDPQ010000387         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_509633, whole genome shotgun sequence.
ACCESSION   DATDPQ010000387 DATDPQ010000000
VERSION     DATDPQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509633"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1165
                     /gene="mutY"
                     /locus_tag="VI520_08620"
     CDS             110..1165
                     /gene="mutY"
                     /locus_tag="VI520_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HLF37982.1"
                     /translation="MAAGLTKDLQAWFVKSGRDLPWRRTPDPYAIWISEVMLQQTRVE
                     TAVPYYERFIKRFPDVHSLAAASEEEVLRLWEGLGYYSRARHLHQAATQVVARFGGRI
                     PESPDRLQELPGVGRYTAAAVAAIAFGQDTLALDGNLRRVLARLFDIHLDPRSPAGGR
                     ELQRQAAAILPRGKASEFNQGLMDLGALICKPRAPACPSCPLARYCLARRRATQEQLP
                     VRGPRRSIPDRLAVAAVWEEDGKVLIRQNPSGGLLAGLWGFPGGLLAEGEDERAGLTR
                     LIEQQLEIRIRLGEPLPRLKHSYSHFRVTLQPFKCQAAGRRLRERAGTRWVGVELLGR
                     LPMGRLDRQLADRLTAG"
     gene            1321..2568
                     /locus_tag="VI520_08625"
     CDS             1321..2568
                     /locus_tag="VI520_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37983.1"
                     /translation="MPQSPLLAALERKSGLIAYVGPDGNVYTIDQGGGNQSKITEDAH
                     PAEEGVRRFYDFPTWSAADNKLAFVGFEVDQDGRTNAGIFTVEPEDQPPVEVYSSDQH
                     LPFYLYWSPDGEWVSFLSSSLGSNSMAMQLTSAAGGETSLIDTGRPYYWAWAPEGMQV
                     LAHVGGSSATNPGAARISFLEMDPFVRAVGLRIEPTSFQAPAYSPDGEYVLFAGDSGD
                     GQPQLVLADSAGEVLRSLVDYDGSIAFSWAPRGDYAAYLSGDTSSQALIGDLTFVDLR
                     RPDEPQHIKTDAERVLGFFWAPDGKTVAYFVPVMFSNAAEGEEQTAENSQFLLELHIA
                     EARSGSTRRIAAFQPTQTFLNIFPFFDQYQRSVTIWSPDSNYLVVSAVASAEQQGLFI
                     VPASGDYEPRFLAEGNVGFWSWK"
     gene            complement(2641..>2877)
                     /locus_tag="VI520_08630"
     CDS             complement(2641..>2877)
                     /locus_tag="VI520_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37984.1"
                     /translation="ELSNAEVLQMLGKNSIQVAVEQGLELESVLEQMGVNPRGLAYQR
                     REELPTGEAPASPKASNETERPEKTKRKTAKAQS"
BASE COUNT          497 a          849 c          945 g          586 t
ORIGIN      
        1 tcgagcgctt cattaaagat gggcatgggc gctgctccgg ggcgtgccag ctcggagctc
       61 cgaggctgga agccggcgaa ttatagcttg cgaccggtat acttcgcgca tggccgccgg
      121 attgacgaag gatctgcaag cttggtttgt gaaatccggg cgggaccttc cctggcggcg
      181 gacccccgat ccttacgcga tctggatttc ggaggtgatg ctgcagcaga ctcgggtcga
      241 gacggccgtg ccttactacg aacgattcat aaagcgcttt ccggatgtgc actcgctggc
      301 ggcggcctcc gaggaagagg tgctgcggct ctgggagggg cttggctatt actcccgggc
      361 tcgtcacctg catcaagccg cgactcaggt cgtggcgcga tttggtgggc ggattcccga
      421 aagtccggac cgcttgcagg agttgcccgg cgttggacga tataccgcgg cggccgtcgc
      481 ggcgatcgcc ttcggccagg ataccctggc actggatggc aacctgcggc gggtgctggc
      541 ccgcctgttc gatatccatc tcgacccacg cagccccgcg ggagggcggg aactgcagcg
      601 gcaggcggcc gcgattctgc cgcggggaaa ggcctccgag ttcaatcagg gcctgatgga
      661 cctcggtgcg ctcatttgca agccgcgggc accggcttgc ccgagctgtc cgctggcccg
      721 ttactgtctg gcgcgccggc gggccaccca ggagcaattg ccggtacgcg ggccacgtag
      781 atcgatcccc gatcgcttgg cggtggccgc ggtctgggaa gaggacggaa aggtgctgat
      841 ccgacaaaat ccgtccggcg gtctgctagc gggactctgg ggttttccag gtggactctt
      901 ggcggaaggc gaggacgaac gggccggatt gacccggctg atcgagcagc agctggagat
      961 ccggattcgg ctgggcgagc cgctgccgcg attgaaacat agctacagcc atttccgggt
     1021 cactctgcag ccgttcaagt gccaggccgc cgggcgtcgg ttgcgcgaac gggcgggcac
     1081 tcgctgggtt ggagtggaac tgcttggaag gctcccgatg ggaaggctag accgtcagct
     1141 ggccgatcga ctgacagcag ggtgagggtt tgccgcggtt cttctgggac cttaatcaga
     1201 cgttctgagg cctcatgtta caatctgccg acttcctggg atctgggcaa ccttatgtgg
     1261 cgctggcttc tcccgattgt tgcggtggcc gtggcgggtt gctccgagag gggcctgcga
     1321 ttgccgcaaa gcccgctgct ggcagccctg gagcgcaaga gcgggttgat tgcgtacgtc
     1381 ggtcccgatg ggaacgtcta cacaatcgac caaggcggcg gcaatcaatc caagatcacg
     1441 gaagatgccc atccggccga agagggtgtg cggcgtttct acgacttccc cacctggtcc
     1501 gcggcagaca ataagctggc tttcgttgga ttcgaggtcg accaggatgg gcggaccaac
     1561 gccggcatct tcactgtgga acctgaagac cagcccccgg tcgaggttta cagcagtgac
     1621 cagcatctgc ccttctatct gtattggtcc ccggatggtg agtgggtgag tttcctcagt
     1681 tccagtctgg gaagcaattc gatggcgatg cagctgactt cggcagcggg cggagaaact
     1741 tcgctcatcg ataccggccg cccgtactac tgggcctggg ctccagaagg gatgcaggtg
     1801 ctggcccacg tcggggggtc gtccgcgacc aatccaggcg cagctcgaat ctcattcttg
     1861 gaaatggacc cctttgtgcg agcagtcggg ctgaggatcg agcctaccag ctttcaggcc
     1921 ccggcctact ctccggacgg cgagtacgtg ctattcgcgg gcgatagcgg ggacggccag
     1981 ccccaacttg tgctcgcaga ttcggcaggc gaagtgctgc gctccctggt agattacgac
     2041 gggagcatcg ccttctcctg ggctccccgg ggggattatg cagcctacct cagcggggat
     2101 accagcagtc aagcgcttat cggcgatctg acattcgtgg atctgaggcg accggacgag
     2161 ccgcagcata tcaagaccga tgccgaacgg gtgcttggtt tcttttgggc accggacggc
     2221 aagacagtgg cgtatttcgt gccggtcatg ttttctaacg cggccgaggg cgaggagcaa
     2281 accgccgaaa acagccagtt cctgttggag ctgcacatcg cggaagcccg cagcggcagc
     2341 acccggcgga tcgcagcgtt ccagccgacc caaacgttcc tgaacatctt tcccttcttt
     2401 gaccagtacc aacgctcggt gacgatctgg tcgccggaca gcaactatct ggtggtttcg
     2461 gcggtcgcca gcgcagagca gcaggggctg ttcattgtcc cggcctcggg cgattacgag
     2521 ccgcggttcc tcgccgaagg caatgtcgga ttttggtcct ggaagtagat ttgacctctg
     2581 agaatcaaac gggaggccga tttcggcctc ccgttttctt tacgacgtaa tccagctagc
     2641 ctaactctgc gcctttgctg tcttgcgctt ggtcttttcg ggtcgctcgg tttcgttgga
     2701 agccttcgga ctggcaggtg cctcgccggt gggcagctcc tccctgcgct gataggccag
     2761 cccgcggggg ttgacgccca tctgctcgag cacgctctct agttccagcc cctgttcgac
     2821 agcaacctga atgctgttct tgcccaacat ctgcaagact tcagcgtttg aaagctc
//
LOCUS       DATDPQ010000388         9906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1039403, whole genome shotgun sequence.
ACCESSION   DATDPQ010000388 DATDPQ010000000
VERSION     DATDPQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039403"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VI520_08635"
     CDS             complement(<1..1462)
                     /locus_tag="VI520_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37985.1"
                     /translation="MGEMSSQVREPIHGYATQPALVPPPSKSFEAEALRITPRSRTAQ
                     ILLTYSPAILALLLMLPRLASAEFGLFDDGETVLVAEAIDGGGWAILSEEASAGRFRP
                     VHWLQYSLVYSLSGPQPAAFFLWNALVLVVITVELIGLVQLCGGSTLQAWLSGFLLVL
                     SGPVVENAYTLSKPELLQSALLLGGLISFAGLRKGQGSFRRWGTSGLVSAILFLGGIL
                     TKETALLMLPIAIAWLLAAYLRFRRGGDPEGLRRTFALALAALAAAVAFFLLRGQFLE
                     VSLAGGTYSNNLELSLPRVRDSAIRWSGWLIRDFAYLAPLGLLALIDLTRGRLEQGRL
                     ILASLLWTAAWIAIYLPWEFTVEYYMLPVALGVSVIAGAIVGSVLHRLRGSEVAGRPR
                     WEWAAILPALFLWLTTLPNNLSNARQQLAVDEANAEMMDYLAAGLPPAGTLIVNIQQP
                     NEYVEEIESQLRGLHGLGEVSVEVFDPQRGLPSDTSV"
     gene            complement(1488..2303)
                     /locus_tag="VI520_08640"
     CDS             complement(1488..2303)
                     /locus_tag="VI520_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HLF37986.1"
                     /translation="MKPLLRRLIPDPIWREGSRLYWWWHNRGRHRGAATFSARWRKSR
                     RALESFRQRYRGERCFILGNGPSLRVTDLSLLRGETTFGTNRIYLLFQELGFTTTFYT
                     AVNTLVIEQCATEIRSLNIPKFLTWRARGWVAEDRGVVFLDTDYTGPETFAQDLTSRV
                     FEGGTVTYVAMQLAYYMGFSEVILVGVDHNYASQGPANETVISDGEDPNHFHPDYFGK
                     GFRWQLPDLEASERSYELAREAFERDGRRILDATIGGKLSVFPKVDYASLFGK"
     gene            complement(2486..3229)
                     /locus_tag="VI520_08645"
     CDS             complement(2486..3229)
                     /locus_tag="VI520_08645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLF37987.1"
                     /translation="MKRVALITGASGGIGQAAVQLFTSANWQVVAADRKAAPRPAESV
                     RFVQADVSSPDDVSRVFDELAGQEGRLDALVNNAAIQISKLILTTSVAEWDDVMDSNM
                     RSVYLTAKHGFELLRAARGSIVNVSSVHALATSASIAAYAASKGGLLALTRAMAIEFG
                     RRGVRVNAVLPGAVDTPMLRDSLGRLKGGTLEHKLQALGARSPLGRIGRPEEIAQAIL
                     FLADSEQSAYITGQGLVVDGGALARLSTE"
     gene            complement(3275..4261)
                     /locus_tag="VI520_08650"
     CDS             complement(3275..4261)
                     /locus_tag="VI520_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLF37988.1"
                     /translation="MGYRVLISAPYLIPQFERFREHFSLAGIEVILAEVNERLGETEL
                     LEYVGGVDGVICGDDQFTSQVLERVAPRLKVISKWGTGVDSIDLEVARRLGVKVFNTP
                     GAFTDPVADSVLGYVLAFARNSPWTDRELKGGRWTKGTARALNECALGVVGVGRIGKA
                     VLRRARPFGMKLLGSDIVEIDAGFVEQVGVEMTSLDELLALADFVSLNCDLNPTSHHL
                     MNAERLAMMKVGAVLVNTARGPVVDEPALIEALQSGRLGGAALDVFEQEPLSTDSPLR
                     RMDNVLLAPHNANSSPSAWERVHWNTIGNLFEGLGLSRPEPRPVVDITPVQR"
     gene            complement(4266..6191)
                     /locus_tag="VI520_08655"
     CDS             complement(4266..6191)
                     /locus_tag="VI520_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37989.1"
                     /translation="MRDHLESPNARGRLRAEEGVLIASLCLYLVVLFRTAWVSDDAYI
                     TFRTISNLSSGHGLAWNVGERVQAYTHPLWMFVISAGVYFTHEFYFTSLAVSILVSLA
                     AAAVLARFVATRPMAAVLGLAWLFGSRAYVDYSTSGLENPLTYLLLATYLAIYFRLAD
                     RRWRLIALSTVAALLALNRLDSLLLVLPSLLYAAWRERWNPALRGLALGFLPLLGWEG
                     FSMVYYGFPLPNTAYAKLASGLPPSELWQQGFYYLLNSLNVDPLTPTLILAGLAAAYF
                     MMRRGSVTIALGVLVYLLYVVRIGGDFMGGRFLAAPLVLCAGLLSRSQISDRWTVAAL
                     VVVVAAGFAAPHPTLLSGADYGSQGQAWIDAWGISDERAVYYPSTGLLQARRHVDMPN
                     HAWAHEGLAIRGQAGAAVLPVGAGFFGFFAGPDIHLVDLVGLADPLLARLPATPNPFW
                     RIGHFERRIPDGYLETLRSGENRIVEPNLAEYYDKLSLIVSGPLFDRERWAAIWQLNL
                     GAADSLLRAYADSLPRTVSIHEVELSESVREASSEGIAFSPDGLRILFGRAEHARRLR
                     LRLDRRTAYLAVFSLGSEEVTRKLIPAGDPGEAEVANYEVGVSARAADRGYDALALFP
                     ANAQAELRLEYVEILED"
     gene            complement(6235..7785)
                     /locus_tag="VI520_08660"
     CDS             complement(6235..7785)
                     /locus_tag="VI520_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37990.1"
                     /translation="MRRWPERSPLHPFLLATYPVLALLASNLGQVRPGIANRALIVSI
                     LLGGMTVLGFRALLRDWPRAALLASWGLLLFYSYGHVYNELRSVQVMGTVLGRHRFLL
                     SAWALLGLGGAWLILRRPAAPEWLTAMLNFGLLLAIAIPIGKILLSGVGPVAASTKAG
                     VNAMELTGLQAPADRPPPDIYFIILDAYARADTLRDQFDFDNGAFLDALSEMGFYVAE
                     CSQSNYAQTELSLAATLNMQYLSDLIDESIQGEAARAQLWPLIRHSAVRLLLEGLGYR
                     TVAFETGYYWTEWEDADVYLAPQRGLFAGMSAFEGTLLRSTAAWALIDALPVLPPFLQ
                     RDLDRSTDAHRDRLLYVLDQLGRTPEIPGPKFVFAHIVSPHRPFVFDAEGNPVDDEYT
                     WTHSNLGLDAYREGYRQQVAYLNSRMVSILRGIVEGSNGQAVIIMQGDHGPEEGSSQD
                     RMRNLDVIHLPGGDSPTLYPSISPVNAMRIALNDALDARLPLAEDISRFSTYEAPFDY
                     SQIASECQ"
     gene            complement(7782..9176)
                     /locus_tag="VI520_08665"
     CDS             complement(7782..9176)
                     /locus_tag="VI520_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37991.1"
                     /translation="MRKNADIEIHPASFRDPSGFVFRRNGRLYRQVNRAYAGHYEALV
                     GSGLYDRLIQQGRLLPHAEVDAPAARPEDAFKVLEPEPLDFVSYPYEWCFGQLKDAAL
                     ATLDTQRMAIEHGMTLKDASAYNVQFRQGRAQLVDTLSFEVLRKGEPWVAYRQFCQHF
                     LAPLALMAYRDFRLGELLRNHIDGIPLDLASRVLPRRTWLSPGLLVHIHLHASAQRRY
                     AGKSVLAKRAGRRMSRQALLGLVENLEAAVRRLHWRPVGTEWANYRQFHGYSEADLAE
                     KCRLVGEFLDQVRPVSVWDLGANVGTFSRIASQRGIATIAFDFDPAAVEHNYLKAKKE
                     GDPRLLALVMDLTNPSPALGWAGKERMSLAERGPVGALLALALVHHLAISNNVPLGMI
                     AEFLAGLSSWLLIEFIPKEDPQVQRLLASRADIFSEYNFEGFEAAFSPHYEFRRRDPV
                     GDSGRWLYWMERGE"
     gene            complement(9160..9378)
                     /locus_tag="VI520_08670"
     CDS             complement(9160..9378)
                     /locus_tag="VI520_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37992.1"
                     /translation="MNKLEIARILSSLLASVFARPVQYLDPGSGSYLLQLLIAGILGA
                     LFALRLYWRQVKGFFLKLLKRGGDEEER"
     gene            complement(9470..>9906)
                     /locus_tag="VI520_08675"
     CDS             complement(9470..>9906)
                     /locus_tag="VI520_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HLF37993.1"
                     /translation="GQIAMNEVLLYDVETIEAGFYLQTHSTNPLLRPETISRAISRFF
                     DAYPDHDSLFSVTRLQQRLWDEYSQAVNHDPAVLQRTQDLPPLFVENSCLYIFERESF
                     LRRRNRIGEKPLMFEVEPLEAWDIDEEHDFAIAERLLTNWRD"
BASE COUNT         2059 a         3214 c         2922 g         1711 t
ORIGIN      
        1 cgaccgaggt atcggagggg agacctcgct gtggatcgaa cacctccacg ctgacctccc
       61 caagcccatg caaccctcgt agttgactct cgatctcctc aacatactcg ttgggctgct
      121 ggatgttcac gatgagcgtt cctgccggag gcaagcccgc cgcgaggtaa tccatcatct
      181 cggcgttggc ctcgtccaca gcgagctgct gtctggcgtt acttagattg ttgggaagtg
      241 tggtcagcca gagaaagaga gctggaagaa tcgcggccca ctcccagcga gggcgtcctg
      301 ccacctcgct tcccctcaac cgatggagca ctgaccccac gatcgcaccc gcgattaccg
      361 agacgcccag cgccaccggc aacatgtagt actcgacggt gaactcccag gggaggtaga
      421 ttgcgatcca cgcggcggtc caaagcagcg aggccaggat caggcgaccc tgttccaacc
      481 gtcctctcgt gaggtcgatg agggcgagca ggcccaatgg cgccaagtag gcgaaatctc
      541 ggatcagcca gccactccag cggattgctg aatccctcac cctgggaagg ctgagctcga
      601 gattgttgct gtaggttccg ccagccagcg aaacctccaa gaactgccct cgcaagagga
      661 agaaggccac cgccgccgcc aatgcggcaa gcgccagggc gaaagtccgg cgcagtccct
      721 ctgggtcgcc tccgcggcgg aaccgcaagt atgccgccaa aagccaagcg atggcgatcg
      781 gcagcatgag cagcgcggtc tccttggtca ggatcccacc caggaagaga atcgcgctca
      841 cgagtccgga ggtcccccac cgacgaaacg agccctggcc tttccgcagg ccggcgaacg
      901 agatcagccc acccagaagc agcgctgact gcaagagctc cggcttggat agggtgtagg
      961 cgttctccac tacggggccg gagaggacca ggaggaatcc gctcaaccag gcctgcaacg
     1021 tcgagccgcc gcaaagctgc accaagccga tcagctccac ggtgatcacg actagcacca
     1081 gcgcattcca gaggaagaag gccgccggct gtggcccgct gagcgaatag accagcgaat
     1141 actgaagcca atgcacgggc ctgaacctgc ctgcactggc ctcctcggag aggatggccc
     1201 agccgccccc atcgatcgcc tccgcaacga gcactgtctc gccgtcatca aacagcccaa
     1261 actcagccga agcgagcctc ggcagcatga gcaggagcgc caggatcgcc ggcgagtagg
     1321 tcagcaggat ttgggcagtt cgggagcgcg gcgtgatcct cagtgcctct gcctcgaagc
     1381 tcttggaggg tggggggacc aaggctggct gcgtggcata tccatggatc ggctcacgga
     1441 cctgtgaaga catctccccc accgagcctt cctgcctctg ggtcgggtca tttgccgaat
     1501 aaggaagcgt agtctacctt gggaaagacg gagagtttgc cgccgatagt ggcatcaaga
     1561 atccggcgac cgtcccgctc gaaggcctct cgggccagct cgtaggagcg ctcggaggcc
     1621 tccagatcgg gaagttgcca gcgaaagccc ttgccgaagt aatccgggtg gaagtggttt
     1681 ggatcctcgc catcagagat gaccgtttcg ttggccgggc cctgactggc gtagttgtga
     1741 tccactccca ccaggatgac ctcggaaaaa cccatgtagt aggccagctg catggccacg
     1801 taggtgacgg tgcccccttc gaagacccgc gaggtcaggt cctgggcgaa cgtctcgggc
     1861 ccggtgtaat cggtgtccag gaagaccacc cctcgatcct ccgccaccca gcctcgggcg
     1921 cgccaagtca ggaacttcgg gatgttgagc gatcggatct ccgtcgcgca ctgttcgatt
     1981 accagggtgt tcaccgcagt gtaaaaggtg gttgtgaacc caagctcttg aaagagcagg
     2041 tagatgcggt tggtcccgaa ggtggtttcc ccccgcagca ggctcagatc ggtcactcgc
     2101 aagctgggcc cattgcccag aatgaagcag cgctccccgc ggtaacgctg tcggaaggac
     2161 tccagggcac gccggctctt cctccagcgc gcgctgaagg ttgcggctcc tcggtggcga
     2221 ccgcggttat gccaccacca gtagagccgc gagccctccc gccagatcgg gtcggggatc
     2281 aaccgccgga gcagtggctt catcttcttt cctcgaacgc tcacgggcct catgccgccg
     2341 gccgcatcgc cacccagttc cagcaatccg tcgcgaagag cggatgggag gcctcccaga
     2401 tcaggcttgc ccgcagcgcg gcctggcgct aggtccaacc tggacccggg aggtcatgcg
     2461 cgagggttcg cgcggatcca tgcggctact ccgtgctcaa ccgggccagc gcgccgccgt
     2521 ctactaccag cccctgaccg gtgatgtacg cggattgctc gctatccgcc agaaagagga
     2581 tcgcctgagc gatttcctcg ggtcgcccaa tccgtccgag gggcgaccga gccccaaggg
     2641 cctgcagctt gtgctccagg gtcccgccct tgagccgacc aaggctatcc ctcagcattg
     2701 gagtgtcgac cgccccgggc agcacagcat tcaccctgac ccccctgcgc ccgaactcga
     2761 tggccatggc ccgagtcagt gccaacaagc cccctttgct cgccgcatag gccgcaatac
     2821 tcgccgaggt tgctagcgca tggacagaac tcacgttcac gatcgacccg cgagccgctc
     2881 ggagcaattc gaacccgtgc ttggccgtta gatagactga gcgcatgttc gaatccatga
     2941 cgtcgtccca ctcggcgacc gaggtcgtga ggatcagctt gctgatctgg atggccgcat
     3001 tgttgacaag ggcatccagc ctgccttcct gcccggcgag ctcatcgaaa acccgactca
     3061 catcatcggg ggacgagacg tccgcctgca cgaaacggac actttcagcc gggcggggcg
     3121 cggctttccg atccgccgcc acaacctgcc aattggcgga ggtaaaaagc tggacggcgg
     3181 cctgaccgat tcctccggag gccccggtga tcaaggctac tcgcttcatt gacgacgccg
     3241 tcgcctcaag actcccaagc tatcaccaca gtggtcagcg ttgcaccggt gtgatgtcga
     3301 ccacgggtct cggctccggg cgggagagac cgagcccctc gaaaagattg ccgatggtgt
     3361 tccaatgcac ccgctcccag gcgcttgggc tgctatttgc gttgtggggg gccaagagca
     3421 cattgtccat cctgcgtaac gggctatccg tggagagagg ctcttgctca aatacgtcca
     3481 gcgccgcccc gccgaggcgg ccgctctgca gcgcttcgat cagagctggt tcatcgacca
     3541 cgggtccacg cgcggtattg accagcactg cacccacctt catcatcgca agccgctcgg
     3601 cgttcatcag gtggtgggag gtcgggttga gatcgcagtt caggctgacg aagtcagcca
     3661 gggcaagcag ctcgtccagc gaggtcatct caacacccac ctgctccaca aatccggcgt
     3721 cgatttccac gatgtcactc cccagcaact tcatgccgaa aggtcgtgcc cgtcggagga
     3781 cggcttttcc gattcgtccg acgcctacaa cccccagcgc gcactcattc agcgcgcgtg
     3841 ccgtcccctt ggtccatcgg ccgcctttca gctcgcgatc ggtccagggc gagtttcggg
     3901 caaaggccag gacgtacccg agcaccgagt cggccacggg atcggtgaag gcgccaggcg
     3961 tgttgaagac cttgactccg agccgtcgtg ccacctccag gtcaatcgag tcgaccccag
     4021 ttccccactt ggagatcact ttcaggcggg gggccacccg ttccaacacc tgggacgtaa
     4081 attggtcgtc gccacagatc actccgtcta cgccgccgac gtactcgagc aactcggttt
     4141 cacccaaccg ctcattcact tccgccagga tcacctcgat cccggccaga ctaaagtgct
     4201 cgcggaaccg ctcgaactgc gggatcaaat acggcgccga tatcaggaca cggtatccca
     4261 ctcggctaat cctccaagat ctccacgtac tccagtcgga gttcagcctg tgcattggca
     4321 gggaaaagcg ccaaggcgtc gtacccccgg tcggccgccc tagcggatac tccaacctca
     4381 taattggcga cctctgcctc tcccggatcg cccgccggga tcagcttcct ggtgacctcc
     4441 tcggatccaa ggctgaagac cgccagatag gccgtcctcc gatccaatct gagcctcagt
     4501 cttcgtgcgt gttcggcccg tccaaacaga atacgcaagc catccggcga aaaggcgatc
     4561 ccctcactcg acgcctcgcg caccgactca gacagctcga cctcatggat cgaaacggtg
     4621 cgaggtaacg aatccgcgta ggcgcgcagc agggaatccg cggcgccgag gttgagctgc
     4681 cagatggcgg cccagcgctc ccggtcgaac aacggcccgc tgacgatcaa cgagagcttg
     4741 tcataatact cggcgagatt aggctccaca atccggttct ctccggaccg caacgtctca
     4801 aggtacccat ccgggatcct gcgttcgaaa tggccgatcc gccagaatgg gttcggcgtc
     4861 gcaggtaatc tggctaagag aggatcagcc aggcccacca ggtcgaccag atgaatatca
     4921 ggcccggcaa agaaaccaaa gaaacccgct ccgaccggca ggaccgcggc ccccgcctgt
     4981 ccacggatcg caagcccttc atgggcccag gcgtggttgg gcatgtccac atggcggcgg
     5041 gcttgcagca ggcctgtgct cgggtagtag acagcccgtt cgtccgagat cccccaggca
     5101 tcgatccatg cttggccctg acttccatag tctgccccgc tgagcagggt cggatgcgga
     5161 gccgcgaaac ccgccgccac caccacaacc aatgccgcca cagtccaccg gtcgcttatc
     5221 tgcgagcggc tcagcagtcc tgcgcacaac accagcggcg ctgcgaggaa gcgaccaccc
     5281 atgaagtcac cgccgatgcg gaccacgtac agcaggtaga ccaaaacccc tagggcgata
     5341 gtgaccgagc cccgtctcat catgaagtag gccgccgcaa gtccggcaag gatgagggtc
     5401 ggcgtcaacg ggtcgacgtt cagtgaattc aacaggtaat agaagccctg ctgccacagc
     5461 tcagatgggg gcagcccgga ggccagcttg gcgtaggccg tgttgggcag gggaaaccca
     5521 tagtagacca tcgaaaaccc ctcccagccc aggagcggca gaaatcctaa cgcgagcccg
     5581 cgcagggctg ggttccagcg ttcccgccag gccgcataga gcagcgaggg aagaacaagc
     5641 agcaggctgt caagtcgatt gagggcgagc agcgccgcca cggtcgatag cgcgatcaac
     5701 ctccagcgcc ggtcggccaa ccggaagtaa atcgccaggt acgtagccag cagcaggtag
     5761 gtgagggggt tttccagtcc tgaagttgag tagtcgacgt aagcccgaga cccgaaaagc
     5821 caagccaggc cgagcaccgc cgccatcggc ctcgtcgcca caaagcgggc caggacagca
     5881 gccgccgcca gggacaccag aatcgagact gcgaggctgg tgaaatagaa ctcatgagtg
     5941 aagtagaccc cggccgaaat gacaaacatc cagagcggat gcgtgtaagc ctgcacccgc
     6001 tcacccacat tccaggccag cccatggccg ctggaaaggt tgctgatcgt gcggaacgtg
     6061 atatatgcgt cgtcgctcac ccaggcggtg cggaaaagca caactaggta caggcagaga
     6121 gaggcgatga gcacgccctc ctcggcgcgg agccggcctc gggcgttggg gctctctagg
     6181 tggtctcgca ttccggattg aatcagtgcc ctggtcccgc ttcagaaacc atcactactg
     6241 gcattcgctc gcaatctggc tgtaatcgaa gggcgcctcg taggtcgaga atctgctaat
     6301 gtcctctgcg agtggaaggc gagcatcgag agcatcgttc aacgcaatgc gcattgcgtt
     6361 caccggagaa atagaagggt agagggtcgg gctgtctccg cccggcagat ggatcacatc
     6421 caggttccgc atccgatcct ggctcgaacc ctcctctggc ccgtggtctc cctgcatgat
     6481 gatcacggct tgcccatttg atccttcgac aatccccctc agtatggaca ccatccggct
     6541 gttcaaatag gcgacctgct ggcggtagcc ctcacggtag gcgtccagac caagattgga
     6601 gtgggtccag gtgtattcat cgtccaccgg attgccttca gcatcaaaga cgaacgggcg
     6661 gtgcggcgag acgatgtggg caaagacgaa cttgggtcca gggatctcgg gcgtcctccc
     6721 caactggtcg aggacataca gcagccgatc gcggtgcgcg tcggtcgacc ggtccaggtc
     6781 ccgctgtaga aacggcggca acacggggag ggcatcgatc agtgcccagg cagccgtaga
     6841 tcggagcagc gtcccctcga acgcgctcat tcctgcgaag agtcctcgct ggggcgccag
     6901 ataaacgtcg gcgtcctccc actcggtcca gtaataccct gtctcgaatg ccaccgttcg
     6961 gtatccaagt ccctccaaca acaatcgcac tgcgctatga cggatcagtg gccagagctg
     7021 agcccgagcg gcctcgcctt ggatgctttc gtcgattaag tcgctcaagt actgcatgtt
     7081 cagggtcgcg gcgagcgaca gctcggtctg ggcatagttc gactggctgc actccgccac
     7141 ataaaacccc atttctgaca gcgcatcgag gaaagccccg ttatcgaagt caaattggtc
     7201 acgcaatgtg tcggcccggg cataagcatc caggatgatg aagtagatgt ccggcggtgg
     7261 ccgatctgcc ggcgcttgca gccccgttaa ctccatggcg ttcacgccgg ccttcgtgga
     7321 agccgcgacc gggccaactc cgctaagtag gatcttaccg atcggtatgg caattgctaa
     7381 cagcagcccg aaattgagca tggcagtgag ccattccgga gccgccgggc ggcgcagaat
     7441 cagccaggcc cctccgagcc ccaggagcgc ccatgctgaa agcagaaaac gatgtcggcc
     7501 taatacagtg cccatcacct ggacagagcg caactcgtta taaacgtgac cataggagta
     7561 gaaaagcagg agtccccagc ttgctaggag cgcggcgcgc ggccagtctc tcagcaatgc
     7621 gcggaagccg agcaccgtca tccccccaag cagaatcgag acgatcaaag ctctgttcgc
     7681 gatcccgggc cgaacctgac cgaggttgct ggccaacagc gcgagcaccg ggtaggtcgc
     7741 cagcagaaac ggatgcagtg ggctgcgttc cggccagcgc ctcactctcc ccgctccatc
     7801 cagtacagcc agcgaccgct gtcgcctacg ggatcacgac gccggaactc ataatgcggg
     7861 ctgaaggcgg cctcgaagcc ctcgaagttg tattcagaaa agatgtctgc ccggctggcg
     7921 agcagccgct ggacttgcgg atcttccttg gggatgaact cgataagcaa ccagctgctc
     7981 agtccggcca agaactcggc gatcattcca agcggcacgt tgttcgagat cgccaggtga
     8041 tgcactaacg ccagagctaa cagtgcgccg accgggcctc gctcggctaa cgacatccgt
     8101 tccttccccg cccaccccag ggccgggctg ggattggtga ggtccatgac cagtgcaagc
     8161 aggcggggat ctccttcttt ctttgcctta aggtaattgt gttcgactgc ggcgggatcg
     8221 aagtcgaacg cgattgtcgc gatccctcgc tggctcgcga ttcggctgaa ggtgcccacg
     8281 ttcgcgccca gatcccaaac gctaaccggc cggacctggt cgaggaattc gcctactaac
     8341 ctacacttct cggccagatc ggcttcagaa tacccgtgaa actgccggta gtttgcccat
     8401 tccgtaccca ctgggcgcca gtggaggcgc cgcaccgcag cctccaggtt ctccaccagc
     8461 ccgagcaacg cctgccggct catccgccgc ccggcccgct tcgccaacac ggatttcccg
     8521 gcgtaccgcc gctgcgccga ggcgtgaagg tgtatatgca ccagcagccc gggcgacaac
     8581 caggttctcc gcgggagcac ccggctcgcc aggtccagcg ggattccatc gatgtggttt
     8641 cgcaacaatt caccgagtct gaaatcgcgg taggccatca aagccagcgg cgccaggaag
     8701 tgctggcaga actgacggta cgcgacccat ggctctccct ttcgcaggac ctcgaacgag
     8761 agcgtatcca ccagctgagc ccgcccctgg cggaactgca cgttgtaagc actggcgtcc
     8821 ttgagggtca tgccgtgctc aattgccatc cgctgagtgt ccagcgtggc aagtgcggca
     8881 tccttcagct ggccgaagca ccactcatag gggtacgaca caaagtcaag cggctccggc
     8941 tcaagcacct tgaaggcgtc ctcgggccgg gcagccggcg cgtccacctc cgcgtgcggc
     9001 agcagacgcc cttgctggat caatcggtcg tagaggcctg atcccaccag cgcctcataa
     9061 tggccagcgt aggcgcggtt gacctgtcgg tagagccggc cattcctccg gaagacaaaa
     9121 ccgcttggat cccgaaacga agccgggtgg atctcgatgt cagcgttctt cctcatcccc
     9181 accccgcttc aagagtttga ggaagaatcc tttgacttgc ctccagtaca gcctcagcgc
     9241 gaaaagcgcg ccaagaattc cggcaatgag cagctgaagc aggtagctcc cagatccagg
     9301 atcgagatac tgaaccggtc gggcgaacac ggaagccagc aggctggaca gaatcctggc
     9361 aatttccagc ttgttcacga tatcgatccc catggcatgc ataggtaagg cagcatagag
     9421 cgttgaccct cctgatcgca gcgactccgg ccacaagcct cgggccgact cagtccctcc
     9481 agttcgtgag cagcctttcg gcgattgcga aatcgtgctc ctcgtcgata tcccaggctt
     9541 ccagcggctc aacctcgaac atcagcggct tctcgccgat tcggtttctc cgccgcaaga
     9601 agctttcccg ttcaaaaatg tagaggcagg aattctctac aaagaggggc ggcaagtcct
     9661 gcgtgcgctg caggactgcc gggtcgtgat tgacggcctg gctatattcg tcccacaagc
     9721 gctgctgcag gcgggtcacc gaaaacaaac tgtcgtggtc gggataggca tcgaagaaac
     9781 gcgagatggc gcgcgaaatg gtctccggtc gcaacagtgg attcgtgctg tgggtctgca
     9841 gatagaaccc agcttcaatt gtctccacgt catacagaag gacctcgttc atggcaatct
     9901 gcccgt
//
LOCUS       DATDPQ010000389         5842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3115803, whole genome shotgun sequence.
ACCESSION   DATDPQ010000389 DATDPQ010000000
VERSION     DATDPQ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115803"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VI520_08680"
     CDS             complement(<1..729)
                     /locus_tag="VI520_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLF37994.1"
                     /translation="MDLAEFLRMAWESIVNSKLRSLLTILGIIIGVASVIVMIGVSAG
                     TEAAIEERITGLGSNLLFVQSGFNRFGPGERSVEFSGPVGQAAGGLVYDDAFAIAEQV
                     NGVVAVAVEQSSVQTVKVGNVSLDSVSIVGSTSDYPSVRDMAIEIGRYFTQQEVDGKH
                     KVAVLGRSLAEELFGDSDPIGQTVNVGGTKLRVIGVFEERGLVSGVDFDSQLYTPISV
                     VFQKFTPSLFARIIGDRVRMIYVEV"
     gene            complement(716..1456)
                     /locus_tag="VI520_08685"
     CDS             complement(716..1456)
                     /locus_tag="VI520_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLF37995.1"
                     /translation="MPILQIIDVSKVYGEGEISVRALEAVSLSIEAGEFVAVMGPSGS
                     GKSTLLNILGCLDRPSAGRYLLDSVDVSQMDRAELARIRNERIGFVFQSYHLLARTSS
                     LKNVVAPLLYSRNHGRTKEEKLGMARQALEAIGLGDRLEHQPQQLSGGQQQRVAIARA
                     LVTDPAFILADEPTGNLDSQTGQEILTLLADLHRQGRTILIITHSEEIAALTDRTVRL
                     RDGRVEQISRNRSGADTLAVNEVPNGSR"
     gene            complement(1456..2841)
                     /locus_tag="VI520_08690"
     CDS             complement(1456..2841)
                     /locus_tag="VI520_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLF37996.1"
                     /translation="MRIGIGAALLLAVPALAYYLLAYAPAQTTTAEPEFQTSAVRQGD
                     LILYASGSGTLIAGNQVELGFGTSGSVAEVHVQAGDTVEAGEILAEQADREQLEAAVA
                     ADELALVEAQQALEELYASADLVAAQAQLSLANAQAAVDVAQATWQSQQAGYRASSTT
                     IRAAEAELALAKEAMAAAEAEAGKFSSDDPQFAQAYKDYAAAVQRYWKALANLNWYTG
                     HPTELQQAKLDAELALAKAQVGEAERAYSQVQAGPDPDQLASAELRVANAEAELALSR
                     RNLERSVIVAPFAGTIMEVSGGIGDSVAAPFLALADLSRPELEVFLDETDADKISIGD
                     EVEVVFDAFPATIYTGRVVQVDPKLNTQLGVATIKGRVQLDPAEVDGLLVGMNAAIDV
                     IGGRAEGVLLVPVEALRELGPGEYAVFVVVDGEPQLRSVEVGLMDLTFAEIRSGLTAG
                     EIVTTGVVETG"
     gene            complement(3053..3715)
                     /locus_tag="VI520_08695"
     CDS             complement(3053..3715)
                     /locus_tag="VI520_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37997.1"
                     /translation="MKDKSWLKWLVLGVGAAAMVLAGIGWVGTAVAQAAPGPRTHTGS
                     IAGHGGFGHWLVDMQALLAEALGIEVEELEAAQQAANEAAIQQLLDQGQLSEEQADLA
                     SAANRLKSYLDREAFVAEALGISVPDLKAAVAEGQTLSDLLDEQGLDSATYRENLAAA
                     EETAIAQAVEDGVITQEQADQLQDRGPGHFGFGPGHGGFHPGGFFGPGVPERAPEDTL
                     NG"
     gene            complement(3819..4454)
                     /locus_tag="VI520_08700"
     CDS             complement(3819..4454)
                     /locus_tag="VI520_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLF37998.1"
                     /translation="MKILAISDLVDERVYSEHIRVNYPEVELVLGCGDLPLYYLEFVA
                     SMLDRPVLYVRGNHDAPYEETADGRQRTVAEGCLPIDGRMARVLGLTFIGLGGSRRYS
                     PDPPFQFSEGQMRRRLAALLPRLAINRVRHGRFVDVAVAHAPPYGIHDANDPAHVGFR
                     TFLSLMRLFRPRLLLHGHVHQWRHDRPMRSVYLETEVVGVFPVRVIELPDR"
     gene            4581..>5842
                     /locus_tag="VI520_08705"
     CDS             4581..>5842
                     /locus_tag="VI520_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF37999.1"
                     /translation="MSEGSPAAYALAVMDFRRARRKAALREVLTRLSRGQRDLLSYEQ
                     VRDKLRARETPGWTLQDIPLDSIVGSMGRYKDFTRDFLPLERADEGRWARVKVAMSGA
                     AGLPPIEVYRVGGAHFVLDGNHRVSVARELGASHIQAYVREVETKVSLSPDVQPDDLI
                     IKAEQAAFLERTRLDEIRPAADFTVTSAGRYDVLEGQIKAHRHSLRLQRPGGVGDAEV
                     VGSWFDRIYLPAVHSIRRISLLREFPARTEADLYVWITQHQADLERSLGWEIRAESAA
                     ADLAARAGRRLRRIIGRIVEKAAGTLTLAPWVPAPAPAQWPEGVLAAHERAGFTLNIL
                     LPVGGARHDWSAFDQAVLVAQREGARLLGLYVMPSGTEPQRESAREIQLEFERRAQAA
                     GVPCGFAIEVGEIAAQIVDRARWVDLVI"
BASE COUNT         1041 a         1952 c         1796 g         1053 t
ORIGIN      
        1 cacttccacg tagatcatcc gcacccggtc gccgatgatg cgagcgaaca ggctgggggt
       61 gaacttctga aagacgaccg agatcggggt gtagagctgc gagtcaaaat ccaccccgct
      121 caccagcccc cgttcctcaa agaccccgat caccctcagc ttcgtgccgc ccacgttgac
      181 tgtctggcca atcggatctg agtccccgaa gagctcctcg gccaggctcc gccccagaac
      241 tgcgacctta tgtttcccat ccacttcctg ctgggtgaaa tagcggccga tttcgatagc
      301 catgtcccgg acggatggat aatcggacgt gctgcccaca attgatacgg agtcgagcga
      361 aacgttgccg accttgactg tttggaccga ggactgctcg accgccaccg cgaccacccc
      421 attgacctgc tcggcaatcg cgaaggcgtc gtcgtacacc aaaccgcccg cggcttggcc
      481 gaccggcccg ctgaattcaa ccgagcgttc tccgggtcca aatcggttga atccggattg
      541 aacgaagagc aagttcgagc cgaggccggt gattcgttcc tcgatggcgg cctcggtgcc
      601 ggcgctgact ccgatcatca caatgaccga ggccaccccg atgatgatcc cgagtattgt
      661 gagcaaagac cgaagcttgc tgttgacaat gctctcccat gccatgcgca ggaattcagc
      721 gagatccatt cggcacctca ttgacggcca gcgtatctgc ccccgaccgg ttccgcgaaa
      781 tttgctccac ccgcccgtcc cgcagccgaa ccgttcgatc ggtcagggcg gcaatctcct
      841 cgctgtgggt gatgatcagg atcgtgcgcc cctgccggtg caggtccgcc agcaaggtca
      901 ggatctcctg cccggtctgg ctgtccaagt ttccggtggg ctcgtcggcc agaatgaagg
      961 ccggatcggt caccaaggcc cgggcgatcg ccacccgctg ctgttgcccg ccggacaatt
     1021 gctgcggctg atgctccaat cgatccccga gcccaatcgc ctccagcgcc tgccgggcca
     1081 tcccgagctt ctcttccttg gtccggccgt gatttcgcga gtacaacagc ggagccacga
     1141 catttttcag cgagcttgtc cgggccagca ggtgatagga ctgaaagacg aatccgatcc
     1201 gctcgttgcg gatcctggcc agctcggccc gatccatctg gctcacgtcc accgagtcaa
     1261 gcagatagcg cccggccgac ggtcgatcca gacatcccag gatgttcaac agggtgctct
     1321 tcccagagcc ggagggcccc atcacggcga caaactcgcc ggcctcaatg gacaggctga
     1381 ccgcctccag cgctcgaacg ctgatttcgc cttcaccgta aactttcgac acgtcgatta
     1441 tctggaggat gggcatcagc cggtctccac cacgccggtg gtcacgatct ccccggcggt
     1501 caggccagag cggatctcgg caaaggtcag atccatcagc ccgacttcga ccgaccgcaa
     1561 ctggggctcg ccatcaacca ccacgaacac tgcgtactcg cccggcccta actcgcgcag
     1621 cgcctcgacc ggcacgagca gcacgccttc ggcacggccg ccgatcacgt caatcgcggc
     1681 gttcatcccg accaacagcc catccacctc agctgggtcc agctgcaccc gccccttgat
     1741 cgttgctacc ccgagctgcg tgttcagctt cgggtctacc tgcacgaccc ggccggtgta
     1801 gatcgtggcc gggaaggcgt cgaagacgac ttccacctcg tctccgatcg agatcttgtc
     1861 cgcgtcggtc tcatccagaa acacctccag ctcgggtcga ctgagatcgg ccagggccag
     1921 gaatggagcc gcaacgctgt cgccgatccc gccgctgacc tccatgatcg tgcccgcgaa
     1981 aggcgccacg atcaccgacc gctcgaggtt gcgccgggag agtgccagct cggcctcggc
     2041 gttggcgacc cgcagctctg cgctcgccag ttgatccggg tccggtccag cctgaacctg
     2101 tgagtaggcc cgctcggcct ccccaacttg ggccttggcg agggctagct ccgcatcaag
     2161 cttcgcttgc tggagctcgg tcggatgacc ggtgtaccaa ttgagattcg caagcgcctt
     2221 ccagtagcgc tgcacggccg ccgcatagtc cttgtaggcc tgggcgaatt gggggtcgtc
     2281 tgacgagaac ttccccgcct cggcctcggc agccgccatc gcctccttgg ccaaggccag
     2341 ctccgcctcg gcggcccgga tcgtcgtgct gctggcccga tatccggctt gctgcgactg
     2401 ccaggtggcc tgagcaacat ctacagccgc ctgggcgttc gccaggctca actgggcctg
     2461 ggcggcaacc aaatctgccg aggcatagag ctcctccagg gcttgctggg cctccacgag
     2521 ggccagctca tcggcggcga ccgccgcctc gagctgctcg cggtccgcct gttccgccaa
     2581 gatttccccg gcctcgaccg tatcgccggc ttgcacgtga acctccgcga cgcttccgct
     2641 ggtgccgaag ccgagctcaa cttggttccc agcaatcagg gtcccgctgc cgctggcata
     2701 gagaatcaag tcgccctggc gcaccgcgct ggtctggaat tcgggctcgg cggttgtggt
     2761 ctgcgccggg gcgtatgcca ggaggtagta cgccagcgcc ggaactgcga ggagcagtgc
     2821 cgctccaatc ccaattcgca ccggcctgcg actcaatggg ccaactcgga aaggcctgcg
     2881 tatccagtct gcgagcatct ggattgctcc tcgaatctgt ggtcgctcgt tagggtaggc
     2941 gcccggcgtt caggacttgt gaggggccca tgtgaagact gtgtgaatca caactcctcc
     3001 gcggggcggc ccacggagga gttgctaagg aggagaggag aggaggaaga atctagccgt
     3061 tcaacgtgtc ttccggggcc cgttccggaa cgccgggacc gaagaacccg cccgggtgga
     3121 agcccccgtg gcccggtccg aaaccgaagt gcccgggtcc acgatcctgc agctgatccg
     3181 cctgctcctg ggtgatcact ccgtcctcga cggcctgagc gatcgcggtc tcctccgctg
     3241 cggccaggtt ctcccggtag gtagcggaat ccaacccctg ttcgtcgagt aggtcggaca
     3301 aggtttgtcc ttcggcaact gccgctttca agtcagggac gctgatcccg agagcctctg
     3361 cgacaaacgc ctcacgatcc agatagctct tcaggcgatt cgccgcgctg gccaagtcgg
     3421 cctgttcctc gctcagctgc ccttgatcga gcagctgctg gatggcggcc tcgttcgcgg
     3481 cctgctgagc ggcctccagc tcttcgacct cgatcccgag cgcctccgcc agcagcgcct
     3541 gcatgtcgac cagccagtga ccaaacccgc cgtggcccgc gatcgagccg gtgtgcgtcc
     3601 gcggaccggg ggccgcctgg gcgaccgccg tgcccaccca tccaatgccg gccagcacca
     3661 tcgcggccgc gccgacccca agcaccaacc acttcaacca acttttgtct ttcacgcctt
     3721 gctcctttca ggttgtgtga tgcacccgac aggatacgcg gcgtgggttt gcaaaatgtg
     3781 aagctggaat gtcgatccgg taatatccgg ctgaacccct agcgatcggg cagttcaatc
     3841 actcggaccg ggaacactcc cacgacctcg gtctcaaggt agaccgagcg catcggccgg
     3901 tcatgccgcc actggtggac gtggccgtgc agcagcagcc gcggccggaa caaacgcatg
     3961 aggctgagaa aggttcggaa cccgacatgg gccgggtcgt tcgcgtcatg aatcccatag
     4021 ggtggggcat gggccaccgc cacgtcaacg aagcggccgt gccgcacccg gttgattgcc
     4081 aagcgcggca gcagtgcggc aagccggcgg cgcatctggc cctcgctgaa ctggaaggga
     4141 gggtcgggcg agtaccggcg cgagcccccg agtccaatga aggtcagtcc caatactcgg
     4201 gccatccgtc catcgatcgg caggcagcct tcggcgaccg ttcgctgccg accgtcggcg
     4261 gtctcttcat agggagcgtc gtggttgccg cgaacataga gcaccggccg atcgagcatc
     4321 gaagccacga attcgaggta atacagtggg agatccccgc aaccgagcac gagctcgact
     4381 tccggatagt tgactcggat gtgctccgaa tagactcgct catccaccaa atcgctaatc
     4441 gccaggatct tcatctacaa ttcagccctc gtgaacccag aggaagtttc agctatcgga
     4501 acaccgggcg gcctgcgcat gattgtattc gtactcggac cgggcgtgtt catcggcgga
     4561 ttccgggcca agggttctgc gtgagcgagg gtagcccggc agcctatgcc ctagccgtga
     4621 tggatttccg gcgagcgcgc cgaaaggcgg ccctccggga ggtgctcacc cgcctgtcac
     4681 gcggccagcg ggatctgctc tcgtacgagc aggtcaggga caaactcagg gccagggaaa
     4741 ctcccgggtg gacgctccaa gatattccgc tagattcgat cgtgggaagt atgggccgct
     4801 acaaggactt cacccgagat ttcctaccct tggagcgggc cgacgagggg cgctgggccc
     4861 gggtcaaagt cgccatgagc ggcgcggccg gcctgcctcc catcgaagtc tatcgcgtcg
     4921 gcggagccca tttcgttctg gatggaaacc accgggtctc ggtcgcccgt gagctcgggg
     4981 cgagccacat tcaggcctat gtcagggaag tggagaccaa ggtctccctg agtccagacg
     5041 tccagccgga tgatctgatc atcaaggccg agcaagccgc attcctcgaa cggacccgct
     5101 tggacgaaat tcgaccggct gcggatttca ccgtgacctc cgccggtcgc tatgacgtgc
     5161 ttgaagggca aatcaaagcg caccgccaca gcctgcggct gcagcgcccg gggggagtag
     5221 gcgacgcaga agtagtcggc agttggtttg atcgaatcta ccttccggcc gtgcattcca
     5281 ttcgacgaat cagcctgctg cgcgagttcc cggcccgcac cgaggccgac ctctatgtct
     5341 ggattacaca gcatcaggcc gacctcgagc gctccctcgg gtgggagatt cgggcggaat
     5401 cggctgcggc cgatctggcg gcgcgggccg ggcgccgcct ccggcgaatc atcggtcgga
     5461 ttgtggagaa agcagccggc acgctgacgc tagctccctg ggtgccggct cccgctccgg
     5521 cccaatggcc ggagggtgtc ttggccgctc acgagcgggc ggggttcacc ctcaacatct
     5581 tgcttccggt cggcggggcg cggcacgact ggagcgcctt cgatcaggct gtgctggttg
     5641 cccaacggga aggcgctcga ctgttggggc tgtatgtgat gccgtcgggc accgagccgc
     5701 agcgcgagtc ggcccgggaa attcagctcg agtttgagcg gcgagcccag gcggccggag
     5761 ttccgtgtgg tttcgcgatc gaggtggggg aaattgcggc ccaaatcgtc gatcgcgccc
     5821 gctgggtcga tttggtgatc tt
//
LOCUS       DATDPQ010000390         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3635663, whole genome shotgun sequence.
ACCESSION   DATDPQ010000390 DATDPQ010000000
VERSION     DATDPQ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635663"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1411
                     /locus_tag="VI520_08710"
     CDS             230..1411
                     /locus_tag="VI520_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF38000.1"
                     /translation="MPDLKYLIIGGGMAANAAVQGIRSLDPSGSLGLLSSEPDPPYRR
                     PPLSKGLWKGKSLDSIWLGTLDQGVELQLGRTAVGLDPNSKLVRDDQGTEYHYGKLLL
                     ATGGTPRRLAQDGNRVIYFRDLADFRKLRALADTVERFVVIGGGFIGSELTAALAMNG
                     RRVSIIFPEAGIGAREFPPELATFLNEYYRGKGVEVLAGETIDRVEQATGSIRVHTRS
                     GKTLPAEAVVAGLGILPNTKLAVDAGLETDDGIVVKPSLQTSDPAIYAAGDVARFHNP
                     ALDRRMRVEHVDNSVTMGGLAGKSMAGADVRYDHLPYFYSDLFELGYEAVGILDPRLE
                     LVTDWIEPLKKGVIYYLEGGRVRGALLWDTWGQVEAARALIAEPGPFSASDLRGRIRS
                     E"
     gene            1421..2455
                     /locus_tag="VI520_08715"
     CDS             1421..2455
                     /locus_tag="VI520_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HLF38001.1"
                     /translation="MRDILPDLIRWREHDSPIALATVVQTWGSSPRPAGAKMAINAGG
                     EITGSVSGGCVESAVAEEGLQVLKDGRPRLLHFGVADETAWQVGLACGGSLDVFVERL
                     DPEHFEFVRQLVEAEAPAATVTVVKGPPAMVGRKLSLSTEDPSRRLGDLNSGLDQPAV
                     ERARQTEQSEMAELAGSEQPVQAFIDVLPPPPTLVIVGGAHIAIPLAELAKTMGYRTV
                     IVDPRRAFATEARFPGVDRLIPAWPGQAFQEFPLTKSTAVVTLSHDPKIDEPALTLAL
                     PSAAFYVGALGSRTTQAKRLERLRERGLSEANLDRLHGPVGLDLAAESPEEIALSIMA
                     QIVATRNSRA"
BASE COUNT          455 a          795 c          819 g          430 t
ORIGIN      
        1 gggttcggcc tcggccggcc cagtcggggc atcgggaggc accggccaga aggcgaggta
       61 agcacaacca gccaggacta acagtcccgc cagggtcaaa agcgcacgga agaaggccag
      121 gatgtatctc acgccggtgc ccaacccgcc gagcgaattg tagtgcaatc acctgggtcg
      181 cggcgggata gtagaattgc agcacgaccg ctcgagtgga agagcccgga tgccagatct
      241 caagtacctg atcatcggcg gtggcatggc ggcgaacgcc gcggtgcagg ggatccgatc
      301 gctcgatccg agtgggtctc tggggttgct cagctcagag ccggaccccc cttaccggcg
      361 ccccccgctc tccaaaggcc tatggaaagg caaatccctc gatagcattt ggctgggcac
      421 tctcgatcag ggggtcgaac tccagctcgg gcggactgcg gtggggctgg atccgaattc
      481 caagctcgtg cgcgatgatc aggggaccga atatcactac ggaaagctgc tgctggcgac
      541 cggcggtacg cctcgccgac tcgctcagga cggcaatcgc gttatctatt tccgggatct
      601 cgccgatttc cgcaaactga gagccctggc cgacaccgtc gagcggttcg tggtcatcgg
      661 cggcgggttc atcggttcgg agttgaccgc agcgctggcg atgaatggca ggcgggtatc
      721 catcatcttc ccggaagccg ggatcggcgc ccgggagttt ccgcccgaat tagccacctt
      781 cctgaacgaa tattaccggg gaaaaggggt cgaggtcctg gccggcgaga ccatcgatcg
      841 agttgagcag gcaacgggct cgattcgggt ccacacccga tctggcaaga ccttgccggc
      901 cgaggccgtc gtcgcggggc ttggcatcct gcctaacacg aagttggccg tagacgccgg
      961 gcttgaaacc gacgacggga tcgtggtcaa accctccctt cagaccagcg atccggcgat
     1021 ctacgccgcc ggtgatgtgg cccgcttcca caatcccgcg ctggaccgcc gcatgcgggt
     1081 cgagcatgta gacaactcgg tcaccatggg cgggctggca ggcaagtcga tggccggagc
     1141 agacgtgcgc tacgatcacc tgccttattt ctattccgac ttattcgagc tcggttatga
     1201 agccgtcgga atcctcgatc ctcgcctcga actggtcacg gattggatcg agccgcttaa
     1261 gaagggtgtg atttactacc tggagggcgg ccgggtgcgg ggcgccctgc tctgggacac
     1321 ctgggggcag gttgaagcgg cccgcgccct catcgccgag ccggggccct tttcagcgag
     1381 cgatctaagg ggacgcatcc gatcggaatg aactgagcca atgcgcgaca tcctgcctga
     1441 cctcatccgc tggcgtgaac acgattcccc gatcgccctg gcgaccgtcg ttcagacctg
     1501 gggatcttcg ccccgcccgg cgggcgccaa gatggcgatc aacgcgggcg gcgagatcac
     1561 cggttcggtc agcggcggct gtgttgagtc ggctgtagca gaggaaggcc tgcaggtcct
     1621 caaggatggc aggccgcggc tcttgcactt tggagtagcg gacgagaccg cctggcaggt
     1681 tggcctggcc tgtggcggca gcctggacgt cttcgtcgag cggctggacc ccgagcattt
     1741 cgaattcgtg cggcaattag tcgaagccga ggcgccggcc gcgacagtaa cggttgtgaa
     1801 gggaccgccg gccatggtcg ggcggaaact tagcctgagt accgaggacc cctcccgccg
     1861 cctcggcgat ctgaattccg ggctcgacca gccggcagtg gagcgcgctc gacagaccga
     1921 gcaatccgag atggccgagc tcgccggcag cgagcagccg gtgcaggcct ttatcgacgt
     1981 gctcccgccc ccgccaactc tggtgatcgt tggcggcgcc cacatcgcca ttccgctggc
     2041 cgagctcgcc aagacgatgg gctatcggac cgtgatcgtg gacccgcgcc gggcattcgc
     2101 caccgaagcc cgcttccccg gcgtcgatcg cctgatcccg gcctggcctg ggcaagcctt
     2161 ccaggagttc ccgctgacga agtcaaccgc cgtggttacc ctctcgcacg atcccaaaat
     2221 cgacgagccg gccctcaccc tggcactgcc ttcggcggcc ttctatgtag gggccttggg
     2281 cagccggaca acccaggcca agcggctgga gcggctcagg gaacgcgggc tctcggaggc
     2341 caacctcgac cggctgcacg gtccagttgg gcttgatctg gccgccgagt cgcccgagga
     2401 gattgcactc tcgatcatgg cccaaatcgt tgccactcgg aattcgcggg cgtgagctaa
     2461 ggagaaaaac ttggcaccga atctcatgat tcctggacc
//
LOCUS       DATDPQ010000391         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1826728, whole genome shotgun sequence.
ACCESSION   DATDPQ010000391 DATDPQ010000000
VERSION     DATDPQ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826728"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1989)
                     /gene="aspS"
                     /locus_tag="VI520_08720"
     CDS             complement(199..1989)
                     /gene="aspS"
                     /locus_tag="VI520_08720"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLF38002.1"
                     /translation="MLKSHSCGELRAEHAGQQVRLAGWVNRRRDHGGLTFLDLRDRSG
                     VVQAVANPGISSAAHTTAAEVRPEWVLHIAGTVRLRPAGMQNPELPTGAVEVEVHELR
                     VLNPAKTPPFLINEDSEVEEMVRLQYRYLDLRRERMQRNLELRHKVIKYIRDFLDRRG
                     FLEIETPILFKATPEGARDYLVPSRLYPGEVYALPQSPQQLKQLLMVAGLDRYFQIAR
                     CFRDEDQRGDRQPEFTQLDLEMSFVEREDVMALLEQLYSDLVSSLFPERPVQAIPWPR
                     LTYREALDRFGTDRPDLRFGMELKDVSDLAAGSGFQVFEAAIAGGGEVRAINAKGCAG
                     FSRREIEELTQFVAPFGAKGLAYLALPESGEPRSSFAKFVAAERLEALQARLEAAPGD
                     LLLFVADRQAVVLESLARLRSQLAARLGLYDTKLLSFCWVIDFPLVLWNEQEGRWDPS
                     HHLFTAPLPEDVHWLDQEPGRARGQQYDVVLNGGETAGGSIRIHDRALQERIFQLIGL
                     ELETAQQRFGHMLTAFEYGTPPHGGIASGLDRLCMVLAQEPNIREVIAFPKNQAARDV
                     MAGAPSLAEPGALRDLHLQVVTSPPGRPNT"
BASE COUNT          462 a          841 c          788 g          456 t
ORIGIN      
        1 ttgaacctgc ttacgcaggt ggcgtcctgc acgtgggttt cgccttggcc gaagcctacc
       61 gcgtccccca tcgtagcgcg gaccccttat cgttgatgtt ggctcaaaac gatatgccgc
      121 atcgcgtgca cagcagggac actacttggt ataccacgat cgcccgtccc caacaatggt
      181 gatcgccggc ctttggtact atgtattcgg acgccccgga ggtgatgtga cgacttgaag
      241 atgcaagtcg cgcagcgctc ccggttcggc cagcgacggc gcgccggcca tcacgtcgcg
      301 ggcggcctgg ttctttggga aagcgatgac ttcccggata ttgggttcct gcgccaggac
      361 catgcacaac cgatccagcc ccgaggcgat tccaccatgc ggcggggtgc cgtactcgaa
      421 ggcggtcagc atgtggccga agcgctgctg ggcagtttcc agctcgagac caatcaactg
      481 aaagatgcgt tcctgcagcg cacgatcgtg gatccggatg ctcccgccgg ccgtctctcc
      541 cccgttgagc acgacgtcgt attgttgacc gcgggcccgg cctggctcct ggtcgagcca
      601 gtgaacatcc tccggcagcg gtgcagtgaa cagatggtgg ctgggatccc agcgcccttc
      661 ctgctcgttc cacagcacga gcgggaaatc gatcacccaa cagaaggaca gcagcttggt
      721 atcgtacagg ccgagccgcg cagccagttg cgatcgcagc cgggcaagcg attcgagcac
      781 caccgcttgc cggtcggcga cgaacagcag caggtcgccg ggggcagcct ccagccgggc
      841 ctgaagcgcc tccagccgct cggcggccac gaacttggcg aaggatgagc gcggctcgcc
      901 cgactccggc agagccaggt aggccagtcc cttcgcgcca aagggcgcca caaactgagt
      961 gagctcctcg atttcgcgcc ggctgaagcc ggcgcagccc ttggcattga tcgctcgaac
     1021 ttctccgccg ccggcgatcg cggcctcaaa cacctggaag ccgctgccgg ccgccaaatc
     1081 tgaaacgtct ttcagctcca tcccgaagcg caggtcggga cggtcggtac cgaagcggtc
     1141 cagggcctca cgataggtca accggggcca cggtatcgcc tgcaccggcc gctccgggaa
     1201 caggctgctg accaagtcgc tgtagagctg ctcgagcagc gccatcacgt cctcccgctc
     1261 gacgaatgac atctcgaggt cgagctgagt aaattccggc tggcggtcgc cgcgctggtc
     1321 ttcatcacgg aagcaccggg cgatctggaa atagcgatcg aggccggcga ccatcagcag
     1381 ttgcttgagc tgctggggcg actgcggcag ggcatacact tctcccgggt acaggcggga
     1441 ggggaccaag tagtcgcggg caccctccgg ggttgccttg aagaggatcg gggtctcgat
     1501 ctccaggaac ccgcgccgat ccaggaaatc ccggatgtac ttgatcacct tgtggcgcag
     1561 ctcgaggttg cgctgcatcc gttcccggcg caggtccaga taccgatact gcagccgaac
     1621 catctcctcc acctcgctat cttcgttgat gagaaaaggc ggtgttttag ctggattgag
     1681 cacccgcagt tcgtggactt cgacttcgac cgccccggtg ggcaattccg gattctgcat
     1741 gcccgccggg cggagccgga cggtccctgc gatgtgtaat acccactccg ggcgcacctc
     1801 ggccgcggtc gtgtgggcgg cgctcgagat ccccgggttg gcaaccgcct gcacgacccc
     1861 cgaacgatcc cgcaggtcca ggaaggtcag cccgccgtga tcccggcgcc gattgaccca
     1921 cccagccaac ctgacctgct ggccggcatg ttcggcccgc agctctccgc aactgtgcga
     1981 cttgagcatg tctcctccca cttaaagaaa aaacgccgcc cggcaggtgt cccctcgggc
     2041 ggcggatagg ttcgtttccc ggtgctcagc gggaacgcgg gccatccacc cgagggcagc
     2101 cgccgtcggg gttgatagga ttggcatcgt tccgcttccc gccagtcatg agggtcgaat
     2161 tatagcactt agcgcattcg cccagctcat ccccgaaggg taggcaacgg ggcccaaccc
     2221 tcgcggatgt ctcccaggag gccggggctg ctcatgcggg atgaagctca ctattcgagt
     2281 accccatgcc tatgctagcc ttcactggat accatcacga atctgacgct cgacaacgat
     2341 ggggggacgg tctaggcctt ctactcggag ccgtccttgg tgagacctgt ggccgatctg
     2401 ttgaatccgt acatcgccgg ctcgccagtc accgacgcca agatgttctt cggtcgggag
     2461 gacatcttcg actggatcca gcacagcctg gcggggcaat tcgccgacca catcctggtc
     2521 atccacggac agcggcgggt cggcaag
//
LOCUS       DATDPQ010000392         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_514442, whole genome shotgun sequence.
ACCESSION   DATDPQ010000392 DATDPQ010000000
VERSION     DATDPQ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514442"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..341)
                     /gene="rpmE"
                     /locus_tag="VI520_08725"
                     /pseudo
     CDS             complement(153..341)
                     /gene="rpmE"
                     /locus_tag="VI520_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(338..607)
                     /gene="rpmA"
                     /locus_tag="VI520_08730"
     CDS             complement(338..607)
                     /gene="rpmA"
                     /locus_tag="VI520_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLF38003.1"
                     /translation="MAHKKGGGSTRNGRDSQSQRLGVKKYAGEYLQSGNIIIRQRGSR
                     IHPGLNVRKGSDDTLFATADGLLQFDFGPGGRRRANVIPQLETDQ"
     gene            complement(620..1123)
                     /gene="rplU"
                     /locus_tag="VI520_08735"
     CDS             complement(620..1123)
                     /gene="rplU"
                     /locus_tag="VI520_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HLF38004.1"
                     /translation="MKYAIFAHGGKQYLAQEGARIEVDRMPLEVGKPVEFQEILLVSD
                     GRKTEVGSPYVPGAKVQGTIVEHPNGKKIIVFKYIPKHRYRRTKGQRRRLTTISIEAV
                     GIAGADEPAPVSPRKPAPRPAAKPAAKPPAKPPAKRASVKRGPAKPSGQARKTAGKPK
                     SARAKKK"
     gene            complement(1196..>3105)
                     /locus_tag="VI520_08740"
     CDS             complement(1196..>3105)
                     /locus_tag="VI520_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLF38005.1"
                     /translation="FAYATRSGTTIAVSDITVPEAKKAIVARTLEEAEAVRKDYLRGL
                     LTEQEQNERIIELWQNTTTEVAAAVKTGMDPAGNLSTMAISGATKGGFGPISQLAGMR
                     GLMADPAGRIIPLPIRSNFRDGLNTLEYFISTHGARKGLTDTALRTADAGYLTRRLVD
                     VAQDMIINSDDCGTEDGMMIRTADDVAGQDFVSRMLGRVLAKRVVDPKTGEVLFPRGA
                     LLDSKAARQLTTTGVEEVLVRSPLTCELQFGLCAQCYGMDLGRGNMVELGAAVGIVAA
                     QSIGEPGTQLTLRTFHTGGVAAGGDITTGLPRVEELFEARKKPKGEAVTSEIEGTVRI
                     VSSDSPDGQREVQVLDSQLLRDEYTIPGNWSLRIENGDTVETGDTLASRGESTIAASH
                     GGKARVKDRKVTISYEQREQVEYEVPASARLLVEHGQRVSAGQQLTEGSLNPHRILRI
                     LGREACEVYLLTEVQKVYRSQGQNINDKHFEVIIRKMLSKVQISQPGGTSLLPGDLID
                     RLYLHRLNAQILAEGKRPARAMQALLGISKASLSTESFLSASSFQHTIKVLAGAAIEG
                     KVDYLRGLKENVIIGKLIPAGTGFPGEAVVLHDERLDGERPVGPVAELEPVTELVGEE
                     PIPEAETVAAD"
BASE COUNT          496 a          996 c          927 g          686 t
ORIGIN      
        1 tcgagtgcct gcccgacctg ggtgatcccg gcctgggcca gggcctgctc agcgcgcttg
       61 cccagctcca attcggtgat tggggtcaat ggcgacgtgc gtgccacctc ccgctccttg
      121 atctcctcca ggtactcctg ccgtgcttcc agcttccgat agaagcgatc cacctggcct
      181 tcggtgtcga caatgcgctg ctcgccggtg tagaagggat ggcagttgga acagacgtcg
      241 gtgtgaatca gctcgaccgt tgacccggta gtccaggtat tgccgcacga acaaactact
      301 ttggcgttcg ggtaataggt ggggtggatg ccgggtctca ctgatcagtc tccaattggg
      361 gtatgacatt cgcccgccgg cggccgcccg gtccgaagtc gaattgcagc aatccgtcgg
      421 cggtcgcgaa cagggtgtcg tcgctgccct tcctgacgtt taggccggga tgaatccggc
      481 taccacgctg gcggataatg atgttgccgg attgcaagta ctcgccggcg tatttcttca
      541 ctcccaggcg ttgggattgg ctgtcacggc cgttgcgggt cgatcctccg cccttcttgt
      601 gtgccatgtc ggctaccctc tatttcttct ttgcccgagc tgacttgggt ttccccgccg
      661 tcttgcgggc ctggccggac ggtttcgcag gcccccgctt aacactggcc cgtttggcag
      721 gaggtttggc gggaggcttg gcagccggct tggccgccgg tctgggcgcc ggtttccggg
      781 gcgaaactgg agctggttca tccgcgccgg caatcccgac cgcctcgatt gagatcgtgg
      841 tcagtctgcg gcgctgcccc ttcgttcggc gataacggtg cttggggatg tatttgaaaa
      901 cgatgatctt cttcccgtta gggtgctcaa cgatggtgcc ctggaccttg gctccgggca
      961 cgtatggaga ccctacttca gtcttcctgc cgtcggaaac gagcaagatc tcttggaact
     1021 cgacgggctt gcccacttcc agcggcatgc gatcgacctc gatcctggca ccttcctgcg
     1081 cgagatattg tttgcctccg tgggcaaaga tcgcatactt cattgtctag tttgacgtac
     1141 tccggcgccc acttgaacgc ggcgcgaact ctaattataa ccggccggct ccccctcagt
     1201 cggcggccac cgtctcagct tcggggatcg gctcttcccc aaccagctcg gtcactggct
     1261 cgagttccgc taccggcccg accggccgct ccccatccag tcgttcgtcg tggagcacga
     1321 ccgcctcgcc cgggaagccg gtgcccgccg gtatcagctt gccaatgatc acgttctcct
     1381 taaggcctcg caggtagtcg accttccctt cgatggccgc cccggccaac accttgatgg
     1441 tatgttggaa ggaggaggcc gacaggaaag attcagtgct caaggaagcc ttgctgatcc
     1501 ccagcagcgc ttgcatcgct ctggccggcc gcttgccctc ggctaagatc tgagcattca
     1561 gccggtgcag gtagagccga tcgatcaggt cgccgggcag caggctagtt cccccgggct
     1621 gcgagatctg gaccttggaa agcatcttcc gaatgatcac ctcgaagtgc ttgtcgttga
     1681 tgttttgacc ctgcgagcgg tagactttct gcacctcggt caacaggtag acctcacagg
     1741 cctcccggcc gagaatgcgc aggatccgat gtggattgag agatccttcg gtcagctgct
     1801 ggcccgccga cactctttgg ccgtgttcca ccagcaatcg ggcgctcgcc ggaacttcgt
     1861 attcgacctg ctcgcgttgc tcataggaga tcgtaacctt gcggtctttg acccgggcct
     1921 tgccgccgtg gctggccgca atagtcgact cgccgcggct ggccagtgta tcgccggttt
     1981 cgacagtgtc gccgttctcg atccggagcg accaattgcc cgggatcgta tattcgtccc
     2041 tgagcaattg gctatccaag acctggacct cccgctggcc atccgggctg tctgacgaaa
     2101 cgatccggac agttccctca atctcagagg tcactgcctc gcccttcggc ttcttgcggg
     2161 cttcgaagag ctcctcgacc cggggtagac cggtggtgat atctccaccg gctgccacgc
     2221 caccggtatg gaaggtgcgg agagtgagtt gggtcccggg ttcgccgatc gactgggcag
     2281 cgacgattcc gaccgccgcg ccgagctcga ccatgttgcc tcgccccaga tccatgccat
     2341 agcactgcgc acacagcccg aactgcagct cacaggtcag cggcgatcgg actagcactt
     2401 cttcgacacc cgtggttgtg agctgcctgg ccgctttgct atctagcagc gcaccgcggg
     2461 ggaaaagtac ttccccggtc ttcgggtcca caactcgttt ggccagcacc cgtccgagca
     2521 tgcgggacac gaagtcctgc cccgccacat cgtcggcggt ccggatcatc attccgtctt
     2581 cggtgccaca gtcatcggaa ttgataatca tgtcctgggc gacgtcgacc aaacgccggg
     2641 tcaggtatcc ggcgtccgcg gtgcggagtg cggtgtcggt tagacccttg cgcgccccgt
     2701 gggtggagat gaagtactcg agcgtgttca gcccatcccg gaagttggat cgaatcggca
     2761 gcgggatgat ccggcctgcc ggatctgcca tgagcccccg catgccggcc agctgggaga
     2821 ttggtccgaa gcctcccttg gttgcgccgg agatcgccat cgtgctcagg ttccccgccg
     2881 gatccattcc ggtcttgacc gcggccgcga cctcagtggt cgtgttttgc cacagttcaa
     2941 tgatccgctc gttctgctct tgctcggtca gcaggccccg caagtagtcc ttccgaaccg
     3001 cctccgcctc ttccagcgtt ctggcgacaa tggccttctt cgcttccggc actgtaatgt
     3061 ccgaaactgc gatggttgtc ccggagcgag tggcgtaagc gaacc
//
LOCUS       DATDPQ010000393         9419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_257064, whole genome shotgun sequence.
ACCESSION   DATDPQ010000393 DATDPQ010000000
VERSION     DATDPQ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257064"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VI520_08745"
     CDS             <1..637
                     /locus_tag="VI520_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLF38006.1"
                     /translation="GGYQRRELWSDEAWQWRLEAKAEHPVYWQREVDRWLWQRFDALT
                     PLEPFKPVIHVNWHEANAYCAWAGRRLPSEAEWELAASAERAAGGQGVSDHKRRYPWG
                     DEPPNPQRANLDSAVMGCLDVRALPAGDSAFGCRQMIGNVWEWTADTFEAYPGFELDP
                     YDTYSAPSFGQQKVLRGGCWATRSRLIRNTFRNFYTPDRNNIFAGFRTCAL"
     gene            complement(652..3441)
                     /locus_tag="VI520_08750"
     CDS             complement(652..3441)
                     /locus_tag="VI520_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HLF38007.1"
                     /translation="MDRTELRASARTTADETPWHALEATRVAALLEVSSERGLESTRA
                     AERLEQYGPNELAEAPRPGFWVQLLQQFNNFVVIVLIVAALVSALLGDFVEAAAILAI
                     VVLNAALGVVQERRAERAMAALRKLSAPDAQVVRDGRRQVIPGREVVPGDLVLLEAGN
                     YVPADLRLLEAVNLRIEEAPLTGESVSVDKDAKLNVDEHVPLGDRHNMAFSGTLVSYG
                     RGRGLVAATGMRTQLGKIATLLQEVKHEATPLQRRLDQLGKVLGWAALAVCAGVFVVG
                     WVRGFDPLEMFLVAVSLAVAAVPEGLPAVVTITLALGMREMIQRNALIRRLSSVETLG
                     STTVICADKTGTLTQSRMTATRLWVDGKLFEIDGADHSPEAAFRVDGSPVNLNHYPGS
                     LTALWVAALVNDTDFEPGTDGSFRVVGDPTEAALVVAASKAGARRDQLEQVYPRINEI
                     PFDSGRKRMTTVHKVLNPTAEDASPFYDGSLRGWEVVATKGAPDVVLDLCTHYQTIQD
                     QSMPLDAAARERILSANAAMAQQALRVIAVAYRAEPDVPDETTPETVEHSLTFVGLIG
                     MIDPARPEVPPALRRARTAGVRTVMITGDYPDTARAIAEAIGLLEPGYQVITGRALDE
                     MDQAQLQQEIRRTAVFARVSPEHKVRIVDALKQDNQVVAMTGDGVNDAPALKRADIGV
                     AMGITGTDVAKETADMVLTDDNYASIVAAIEQGRVIYANIRKFVYYLLSCNLAEILTI
                     LVAILAGLPSPLAAIQLLWLNLVTDGAPALALGLEPGEPKIMERPPRPAKEPIINREM
                     LGRIGIQTVAIAGVTLSAYLIGLRLYPEAARTMAFATISFSELLRAFTSRSLQIPLLQ
                     IGLFSNRAMFAAVVASAVMLLIVIYVPFLQPIFNTVSLGPEQWRFVFPLLLVPALVAE
                     FSKIMLRRGAAVGQA"
     gene            complement(3521..4099)
                     /gene="phoU"
                     /locus_tag="VI520_08755"
     CDS             complement(3521..4099)
                     /gene="phoU"
                     /locus_tag="VI520_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HLF38008.1"
                     /translation="MRDGDQQAAEAIIAADAHLNRIRFEIEEACLEITATQQPAATDL
                     RQVIAAMNVVVDLERMADHAAGVAKTVLRMGAQPRAEPPAGLEAIAELTSSMLRQVLQ
                     VYAAGDVEAAYQVARMDNRIDQGYQALFRELLEEMARDPTHTTTALYLLFAGHNLKRI
                     ADRVTNVAERVIFTASGEMRELNPEPDDARIS"
     gene            4046..4192
                     /locus_tag="VI520_08760"
     CDS             4046..4192
                     /locus_tag="VI520_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38009.1"
                     /translation="MRVGRDNRFGRLLIAVPQRTNCLRDRLYDKLAKLKDSASDRDQL
                     PIQH"
     gene            complement(4207..4953)
                     /locus_tag="VI520_08765"
     CDS             complement(4207..4953)
                     /locus_tag="VI520_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLF38010.1"
                     /translation="MKIAVISDIHGNLASLQAVWEDLELQRPEGVYCLGDLVGYGAHP
                     NEVIEFVRQREIPTLMGNYDEGVGFDLNDCGCVYKDPELKRLGDLSLAWTRAHTTQDN
                     KLYLQSLPLQIRLDDRRPRLLLVHGSPRRMNEYLYEDRPPATFERIAKLAGCDMLLFG
                     HTHLPYEKRVAGTLFANVGSVGRPKDGDPRACYALVELGRRPRVKLRRVEYDVEAEAA
                     AIEQSDLPNHYAEELRLGRTPAMALAGGVP"
     gene            complement(4975..5853)
                     /gene="pstB"
                     /locus_tag="VI520_08770"
     CDS             complement(4975..5853)
                     /gene="pstB"
                     /locus_tag="VI520_08770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HLF38011.1"
                     /translation="MDERLVGLRDRGVSLAARQANVIALEEREPKTGVEPAPEELALE
                     SRGLRVYYGEFLAIRDVNLSIQRRKITAIIGPSGCGKTTLLRSFNRMNELVPTARTEG
                     EVIFQGQNIYADKVDSVEVRRRIGMVFQKPNPFPKSIYENIAWGARINGFQGSLDGLV
                     EESLKRAALWDEVSDKLDQSGLSLSGGQQQRLCIARAIAVRPDVILMDEPCSSLDPVA
                     TFRIEELMRQLASDYTIVIVTHNMQQAARVSDFTAMMMLGQERAGEMIEFDQTGSIFT
                     RPKDKRTEDYVTGRFG"
     gene            complement(5869..7122)
                     /gene="pstA"
                     /locus_tag="VI520_08775"
     CDS             complement(5869..7122)
                     /gene="pstA"
                     /locus_tag="VI520_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HLF38012.1"
                     /translation="MSQLRRTVAAGEFATNVERRRRVGARWRLIFQVSTIVGIVVLTA
                     LLFNILNQSFGLAAVEFGIEPASLAQNGVPLEQLSRDQLTAIFQANVSPGLYRRYESE
                     KPFAERTQFEVYELVMERVVEPTVVKTWSLSDTILKREAIEAEQAAKHPAARLQFFSW
                     LNPEFVVRPQSSDPLRAGVRTAIFGTLWTIAITIVFAFPIGVGAAIYLEEYAGDTGLN
                     RLIQTNINNLAGVPSIIYGILGLAIFVRGLEPLTSGAVFGYGDDPTTANGRTILSAGL
                     TLGLLILPLIIINAREAIRAVPNSLRRASFGLGATRWQTVWSHVLPGAIPGILTGTIL
                     AISRAIGETAPLVVVGASTFVTFDPDGPFSKFTTLPIQIYQWTARPQAEFRNIAAAAI
                     LVLLILLLTMNAGAVVLRNRYRVRF"
     gene            complement(7119..8051)
                     /gene="pstC"
                     /locus_tag="VI520_08780"
     CDS             complement(7119..8051)
                     /gene="pstC"
                     /locus_tag="VI520_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HLF38013.1"
                     /translation="MTTESLIQPASLMRRPRIREALIQGFLFLCGALSILTTLGIVYE
                     LGKESLGFFGSGADQASLAEFLTGTEWSPQIGKFGILPLLNATLMTSFSAMLLALPLG
                     LSVAIYLSEYASDRARSILKPILEVLAGIPTVVYGYFALTFMTPLLRTIFGKEVVEIY
                     NTGSAGLVIGILILPLVTSMSEDALNAVPRSLREGAYALGATRLETAVKIVVPAALSG
                     IAAAFIVAISRAIGETMVVAIAAGAGPALTLNPFKAAETMTGHIVRISGGDLSYDSID
                     YNSIFAIGLMLFTITLALNILSRRIVNRFREVYE"
     gene            complement(8140..9180)
                     /locus_tag="VI520_08785"
     CDS             complement(8140..9180)
                     /locus_tag="VI520_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HLF38014.1"
                     /translation="MHPNRDFVLLLVAGTLLAACATAAPTGAPTSAPVQVEPTAGVEP
                     TAAPPQAEPAAEGALPEVNPLEVTGDIITAGSSTVFPLSERMAERFEEEGYSGKITID
                     SIGSGGGFERFCVTGETDISNASRPIKDSEVESCRAIGREPVEFRVGTDALAITVSAE
                     NDFVDSLTLEQLALVFSTAETWADVDPSWPAEPILRFSPGTDSGTFDYFVEEVFDSEE
                     APILSSANLQLSEDDNVLVQGVLGSPFSVGYFGYAYYQENADTLKVLSVEGVEPTAEN
                     VDNGSYPMARPLFIYSDGAIMQAKPQVASFINFYLTFVNEEVLDVGYFPASPEALDAA
                     KTAWLQATGMGG"
BASE COUNT         1738 a         3035 c         2921 g         1725 t
ORIGIN      
        1 aggtgggtat cagcgccggg agttgtggag cgatgaggcc tggcaatggc gcctggaggc
       61 gaaggcggaa catccggttt actggcagcg ggaggttgac cgttggctct ggcagcggtt
      121 cgacgcgctg acgccattag aaccattcaa gccggtgatc cacgtcaact ggcacgaggc
      181 caatgcctac tgcgcctggg ctggtcgccg gcttcccagt gaagcggagt gggaactggc
      241 agccagtgct gaacgggcgg cgggtggaca gggagtttca gaccacaagc gccgctatcc
      301 gtggggcgat gaaccgccca atcctcagcg agcgaacctc gactcggcgg tcatgggctg
      361 cctagacgtt cgagccctcc cagcggggga cagtgctttc ggctgccgcc agatgatcgg
      421 caatgtgtgg gagtggaccg ccgacacgtt tgaagcctat ccaggattcg agctggatcc
      481 gtacgacacc tactcggcgc cgtctttcgg ccaacaaaag gtgctccgag gcggctgctg
      541 ggcgacccgg tcccgcctca tccgcaatac cttccgcaac ttctacaccc ctgaccgcaa
      601 caacatcttt gccggctttc ggacctgcgc cctctgagcc gcgcccggcc atcaagcctg
      661 ccctaccgcc gccccgcggc ggagcatgat ctttgagaat tctgccacca gcgctggaac
      721 cagcaagagc ggaaaaacaa aacgccactg ctccgggcca agcgagacgg tgttgaagat
      781 cggctgcagg aacggaacgt agatcacgat cagcagcatc accgccgaag caaccacggc
      841 ggcaaacatc gcccgattgc tgaacagccc gatctgaagc agtgggatct gcagcgagcg
      901 agaagtgaac gcccgcagca gttccgagaa ggaaatggtg gcgaaggcca tcgtccgggc
      961 cgcctccggg tagaggcgca gcccgatcag ataggccgag agggtgaccc ccgcaatggc
     1021 gacggtctga atccctatcc ggcccaacat ttctcgattg atgatgggct ccttcgccgg
     1081 acgaggcggt cgctccatga tcttcggctc gccgggctcc agcccaagcg ccagcgccgg
     1141 ggcaccatcg gtcaccaggt taagccacag cagttgaatg gcggccaacg gagagggcaa
     1201 cccggccagg atagccacca gaatcgtcaa gatctccgcc aggttgcagg acaatagata
     1261 gtaaacaaac ttgcggatgt tggcgtagat cacccgcccc tgttcgattg ctgcgacgat
     1321 gctggcgtag ttgtcgtcgg tgagcaccat gtcggccgtt tccttggcga cgtcggtccc
     1381 ggtgatgccc atggctactc caatgtcggc ccgcttgagg gccggggcat cgttgactcc
     1441 atcgccggtc atcgccacaa cctggttgtc ctgcttcaag gcatccacga tccgcacttt
     1501 gtgttccggc gacacccggg cgaatacggc cgttcgcctg atctcctgct gcaattgggc
     1561 ttggtccatc tcgtccagtg cccggccggt gatgacctga tagcccggct ccagcagccc
     1621 gatggcctcc gcgatggccc gggcagtgtc cgggtagtcg ccggtgatca tcactgtgcg
     1681 gaccccagcc gttcgagccc gccgcaaggc cggagggacc tccggccgag cggggtcgat
     1741 catcccaatc aagcccacaa aggtgaggct gtgctccacg gtttcgggcg tggtctcgtc
     1801 tggcacatcc ggctcggccc ggtaagcgac ggcgatcact cgcagcgctt gctgggccat
     1861 cgcagcattg gcactcagga ttctctctcg ggctgccgcg tcaagcggca tagactgatc
     1921 ctggatggtc tggtaatgag tgcacagatc gagcacgaca tcgggtgctc ccttggtcgc
     1981 gactacctcc cacccgcgca ggctcccgtc gtagaaggga ctcgcatctt cggcggtggg
     2041 attgaggact ttgtggacgg tggtcatccg tttgcggccc gagtcgaagg ggatctcgtt
     2101 gatgcgcggg taaacctgtt ccagctgatc tcggcgggcg cccgccttgc tggcggccac
     2161 cacaagcgca gcctcggtcg gatcgcctac cacccggaag gaaccgtccg tgcccggttc
     2221 gaaatcggtg tcgttaacca gggctgccac ccagagcgcg gtcaatgatc ccggatagtg
     2281 gttcagattg accggagagc cgtccactcg aaaggctgcc tctggcgagt ggtctgcccc
     2341 gtcaatttcg aacagttttc cgtccaccca caggcgggtg gcggtcatcc ggctttgggt
     2401 cagggtgcca gtcttgtcgg cacagatcac cgtggtcgag ccgagggtct cgaccgagga
     2461 cagcctgcgg atcagagcgt tgcgctgaat catttcccgc atgccaagcg ccagcgtgat
     2521 ggtcactacg gccggtagtc cttccggcac cgccgccacc gccaggctca ccgccaccag
     2581 aaacatttcc agagggtcga acccgcgtac ccagccgacg acgaaaactc ctgcgcacac
     2641 tgccagggct gcccaaccca agacctttcc caactgatcg agccggcgct gcagtggggt
     2701 agcttcgtgt ttcacctcct gcagcagtgt ggcgatcttg ccgagttggg ttcgcatgcc
     2761 ggtcgccgcc accagccccc gccccctgcc ataagagact aacgtcccac tgaaggccat
     2821 gttgtgtcgg tcgccgagcg ggacgtgctc gtccacattc agcttggcgt ccttgtccac
     2881 cgagaccgac tcgccggtca agggcgcttc ctcgatcctc agattgaccg cctccaagag
     2941 ccgcagatcc gccggcacat agttgccggc ctccagcagc accaggtcgc cagggacgac
     3001 ctctcggccc gggatcacct gccggcggcc gtcccgcacg acctgggcgt caggtgcact
     3061 cagcttgcgc agggccgcca tggcccgctc ggcccgccgt tcctgcacga cgcctaaggc
     3121 ggcgttcaag acaacgatgg ccaggatggc cgcggcttcg acgaagtctc cgagtagcgc
     3181 cgagaccagg gcggccacga ttagaacgat gactacgaaa ttgttgaact gttgaaggag
     3241 ttgaacccag aatcccggcc ggggtgcttc ggccagctcg ttggggccat actgctcgag
     3301 ccgctcagcc gctctggtcg actccagccc gcgctcgctg gatacttcaa gcagtgcggc
     3361 gacccgggtc gcttccagcg cgtgccaggg ggtctcatcc gctgtggtgc gggccgaggc
     3421 ccggagctca gtccgatcca tagctaaaaa gtgggcggcg ctcgccaccc acagcggcat
     3481 tataccgcag gcaactctgt tccctactta gttgttggct tcaggagatc cgagcgtcgt
     3541 ccggttcggg gttgagctcg cgcatttcgc cgctggcggt gaagatcacc cgctcggcga
     3601 cgttcgtcac ccgatcggcg atccgcttta agttgtgccc ggcgaagagc agatacagtg
     3661 cagttgtggt gtgggtagga tcccgcgcca tctcttccaa cagctcccgg aacagggcct
     3721 ggtagccttg atcgattcga ttgtccatcc gcgcgacctg atacgccgct tcgacgtctc
     3781 cagccgcgta gacctgcaag acttggcgca gcatgctcga agtcagttcg gcgatggctt
     3841 ccaggccggc aggcggctcg gccctgggct gggctcccat cctcagcacg gtcttcgcca
     3901 ctcccgcggc gtggtcggcc atccgctcca aatccaccac gacattcatg gcggcgatga
     3961 cctgtctgag atcggtggcc gccggctgtt gggtcgcggt gatctcgagg caggcctctt
     4021 cgatctcaaa gcgaattcgg ttcaagtgcg cgtcggccgc gataatcgct tcggccgcct
     4081 gctgatcgcc gtcccgcagc gcaccaactg cctgcgcgat cgtctatacg acaagctcgc
     4141 caagttgaag gactcggcgt cggatcgtga ccagctcccg atccagcact gaacgggcgg
     4201 gcaggctcac ggcactccac ctgccagggc catcgccggt gtcctgccaa gccgcagttc
     4261 ctcggcataa tgattcggca gatccgattg ctcgatagct gcggcctcgg cctccacgtc
     4321 atactccacc cggcgcagct tgacccttgg ccggcggccg agctcgacca aggcatagca
     4381 cgcacgcggg tctccatcct tgggccgacc tacactccca acgttggcga acaaagtccc
     4441 ggccacccgc ttttcgtacg gcagatgcgt atgaccaaag agcagcatgt cgcagccggc
     4501 aagtttggcg atccgctcga aggtggctgg cggccggtcc tcatacaggt attcgttcat
     4561 ccggcgcggg ctgccatgca ctaagagcag ccgcggccgc cggtcgtcca gtcggatctg
     4621 caggggcagc gactgtagat agagcttgtt gtcctgggtg gtgtgagccc gggtccaggc
     4681 cagggaaagg tccccgagtc gtttgagttc cggatccttg tacacgcagc cgcaatcatt
     4741 cagatcgaag cccactccct cgtcataatt gcccatcaag gtagggatct cgcgctgccg
     4801 aacaaactcg atcacctcgt tcggatgggc tccatatcca accagatctc ccagacagta
     4861 aaccccctct gggcgctgga gctccagatc ctcccagacc gcttgcagcg aggccaggtt
     4921 tccatgaatg tcggaaatta ctgcgatctt catctcggcc tccgcgtgac tcggctatcc
     4981 gaatcggccg gtaacatagt cttcggtccg cttgtctttg ggccgggtga agatgctgcc
     5041 ggtctggtcg aactcgatca tctcgccggc ccgctcttgg cccaacatca tcatcgccgt
     5101 gaaatccgat acccgggccg cctgctgcat gttatgggtg acgatcacga tcgtgtagtc
     5161 ggacgccagc tggcgcatca gctcctcgat ccgaaaggtc gccaccggat ccagcgagga
     5221 gcacggttcg tccatcagga tcacatccgg tcgcaccgcg atcgcccggg cgatgcacaa
     5281 ccgctgctgt tggccgcccg acagcgacag gccgctctgg tcgagcttgt cgctcacttc
     5341 atcccacagc gcggcccgct tcagcgactc ttcgaccagg ccgtccaggc tgccttggaa
     5401 gccattgatc cgtgctcccc aggcgatgtt ttcatagatg ctcttgggga agggattcgg
     5461 cttctggaag accatgccaa tccggcggcg cacctccacc gaatccacct tatccgcata
     5521 gatgttttgt ccctggaaga tcacctcgcc ttcggtacgg gcagtcggga ccagctcgtt
     5581 cattcggttg aaggaacgca gcagggtggt cttgccacag ccggagggac cgatgatcgc
     5641 cgtgatcttg cggcgctgga tcgagaggtt gacgtcccgg atggcgagaa actcgccgta
     5701 gtacacccgc agcccacgcg attccagcgc cagctcctcc ggcgcaggct cgacccccgt
     5761 cttgggctcc cgctcttcga gggcgatcac gttcgcttgc ctcgctgcca agcttactcc
     5821 ccgatcgcgc agtcctacaa gccgctcgtc cattgtgtgc cgtgtgcctc agaaccggac
     5881 ccggtaccga ttgcgcaaca ccactgcgcc ggcgttcatg gtcagcagca ggatgagtag
     5941 aacaaggatg gccgcggccg cgatgttgcg aaactcggcc tgcgggcggg ccgtccattg
     6001 gtagatctga atcggcaggg tggtgaattt cgaaaacggt ccgtcggggt cgaaagtcac
     6061 gaaggtggag gcccccacca ccaccagcgg cgcggtctct ccaatggccc gggagatggc
     6121 caggatcgtt ccggtcagaa ttccagggat ggcgccgggg agcacgtgcg accacacggt
     6181 ctgccagcgg gtggcgccca ggccgaagct ggcccgccgc aaggagttgg gcaccgcccg
     6241 aatggcttcc cgggcgttga tgataatgag cggcaggatc aacagtccga gggtgagccc
     6301 ggcggagagg atggtgcgcc cattggcggt ggtcgggtcg tcaccatagc caaacacggc
     6361 gccgctggtg agcggctcca acccgcggac gaaaatcgcc agccccagga tcccgtaaat
     6421 tattgacggc accccggcca gattgttgat attggtctgg atcaaacggt tcaacccggt
     6481 gtcgccggcg tactcctcaa ggtagatcgc ggcgcccacc ccgattggaa atgcaaagac
     6541 gatcgtgatg gcgatcgtcc acagcgtgcc gaagatcgcg gtgcgcaccc cggcccgcag
     6601 cggatcggag gactgcggcc tgaccacgaa ctcggggttc agccagctga agaactggag
     6661 ccgggcggcg ggatgcttcg cggcttgctc agcctcgatc gcctcccgct tgagaatcgt
     6721 gtcagagagc gaccaggtct tgacgacggt tggctcgacc acccgttcca taactagttc
     6781 atacacctcg aattgggtgc gctcggcaaa tggtttctcg ctctcgtagc ggcggtagag
     6841 gcccggcgag acattggcct ggaaaatggc cgtcagctga tcccggctga gctgctcgag
     6901 cggcacaccg ttttgggcca gactggcggg ctcaattcca aattccaccg cggccaggcc
     6961 gaagctctgg ttgaggatgt tgaacagcag tgcagtcagc accacgatgc cgacgatggt
     7021 tgacacctgg aagatcagcc gccagcgggc gcccacccgt cggcgccgtt cgacattggt
     7081 cgcaaactcc ccggccgcga cggtccgccg cagctggctc attcgtaaac ttcccggaac
     7141 cggttcacga ttcgccgact gaggatgttg agagcgaggg tgatggtaaa cagcatcaat
     7201 ccgatggcga agatgctgtt gtagtcgatc gagtcgtagc tgaggtcgcc gccgctaatc
     7261 cgaacgatgt gcccggtcat cgtttcggcg gctttgaatg ggttgagggt gagcgccggg
     7321 ccggcaccag ccgcaatagc cacgaccatc gtctccccaa tagcccggga gatggcgacg
     7381 atgaaggccg ccgcaatgcc ggacaacgcg gccggaacca caatcttcac cgcagtctcc
     7441 agccgagtgg cgcccagggc gtaggccccc tcccgcagcg accgcgggac cgcattgagg
     7501 gcgtcctcgc tcatcgaggt caccaggggc aggattagta tcccgatcac caggccggcc
     7561 gaacctgtgt tgtagatctc aacgacttcc ttgccgaaga tcgttcgcag cagcggagtc
     7621 atgaaggtca gggcaaagta gccgtaaacc acggtaggga tcccggccag cacctccagg
     7681 atcggtttca ggatggagcg ggctcgatcc gaggcgtact cactgaggta gatcgcgacg
     7741 ctcaggccca gcggcagcgc cagcagcatg gcagagaagg aggtcatgag ggttgcgttc
     7801 agcagcggca ggattccaaa cttgccgatc tgagggctcc actcggtacc ggtgaggaac
     7861 tcggccagcg aggcctgatc ggccccgctg ccgaagaagc cgagcgactc cttcccgagc
     7921 tcgtagacga ttccgagggt ggtcagaatc gagagtgctc cacacaggaa gagaaagccc
     7981 tggatcaggg cttcgcggat ccgcggcctt ctcatcaagg aagccggttg aatgagagat
     8041 tcagtcgtca tcccaaaccc acaatcttaa caagaccatg cgtaggggct tgggcgctcc
     8101 ctacgcatgg cggcctacgg gcaggcgata cgattcggat cagccgccca tcccggtggc
     8161 ctgcaaccag gcggtcttgg cggcgtccaa ggcctccggg ctggctggga agtaacctac
     8221 atccagcact tcttcattca cgaaggtgag gtagaagttg atgaacgagg ctacctgggg
     8281 tttggcctgc atgatcgccc cgtctgagta gatgaacagc gggcgcgcca tcgggtagct
     8341 gccgttatcc acgttctcgg ctgtcggctc cacgccttcg accgacaaca ccttcagcgt
     8401 gtcggcgttc tcctgatagt aggcatagcc gaagtagcca accgagaagg ggctgcccag
     8461 gaccccctgc accagcacgt tgtcgtcctc actcagctgc aagttcgcgc tgctcaagat
     8521 cggggcctct tccgagtcaa acacctcttc gacgaagtag tcgaaggtgc cgctgtcggt
     8581 ccccgggctg aaacgcagga tcggctctgc cggccagctc gggtccacgt cggcccaagt
     8641 ctcggcagtc gagaacacga gcgccaactg ctctaacgtg aggctatcga caaagtcgtt
     8701 ctccgcgctg accgtgattg ccagcgcgtc ggtcccgacc cggaactcca ccggctcgcg
     8761 cccgatcgcc cggcaggact ccacctcact gtccttgatc ggccggctgg cgttggagat
     8821 gtctgtctcg ccggtgacgc agaaacgctc gaagccccct ccactcccga tgctgtcgat
     8881 cgtgatcttg cccgagtagc cctcctcctc gaagcgttcc gccatccgct cactcagcgg
     8941 aaacacggtc gagctgccgg cggtgatgat gtcgccggtg acctccagcg ggttcacttc
     9001 ggggagcgcc ccttcggcgg ccggctccgc ctgcggtggg gctgccgtcg gctcgacgcc
     9061 cgcggttggc tccacctgca ccggtgccga ggtcggcgcc ccggtgggag cggcggtcgc
     9121 acaggcggcc agcagggtcc cggctaccaa caacaaaacg aaatcccgat tagggtgcat
     9181 tcctgtatct cctcctgaga ttgttcatcg aggaccatcc tagccgggtg ggtttaagac
     9241 ccagtcaagg ttgggttaag tttctttaat cgtgaaggcg ggcggggggg ggcggggtcg
     9301 ccctcatccg aaacttaggc ggcctggaag gccagatgga agacggcgcc cttctcaggt
     9361 gccgggcggt tctcggccca gatcctcccg gcgtgagccc gcaggatgtg atgggcgat
//
LOCUS       DATDPQ010000394         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3638655, whole genome shotgun sequence.
ACCESSION   DATDPQ010000394 DATDPQ010000000
VERSION     DATDPQ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638655"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1052
                     /locus_tag="VI520_08790"
     CDS             102..1052
                     /locus_tag="VI520_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HLF38015.1"
                     /translation="MQSGFVGLLELLGNEAYPLEPASLTELSDLDSVRLAQFRSAWER
                     FGTKRRHAILLQLGELANSKIELSFEAVNRFALQDSDPPTRAQAIGNLWESEDPQLAR
                     QLLDMLRADRVAEVRKAAADALGSFVLLAETEDLAEQLRLEIERGLLKAVRSDPSKEV
                     REGCLQSLGFSTREEVPELIEMAYSSGEGSSLRAALRAMSRSADSHWAPLVVAKLQDP
                     EPGTRFEAVQAAGELDIREASEELVDLLEDVDDRIRKAAIWSLSQVGGSIAIAALEQL
                     QESASESEAQLIQDALDNLEFEDGARELFRLNFDDEDSAA"
     gene            1049..1960
                     /locus_tag="VI520_08795"
     CDS             1049..1960
                     /locus_tag="VI520_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HLF38016.1"
                     /translation="MSGLLSSFADHILPILITAAAGFLAHKLLGLNPRPLSQIAFYVL
                     SPALVFDLLLSADIRGLQVVSMMALATIVVLSIGGFSWLLGRALGLDPKLASAVLLAT
                     AFMNAGNYGLSLNQFVYGESGLAWAGLFFVASMMLTNSLGVYGASRGRRTPLAALREL
                     AKVPAVYAIPLALTARAFDLHLPLALSRPIDLLGTATVPILLLVLGMQIGAGGASGHR
                     GLIAVCVGLRLVVAPLVAWLVAPSLGLDGLARQVGVLESAMPTAVMSTILAIEFDVEP
                     GFVTRVVMLTTLLSPLTLTPLITLLAS"
     gene            complement(1957..>3572)
                     /locus_tag="VI520_08800"
     CDS             complement(1957..>3572)
                     /locus_tag="VI520_08800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLF38017.1"
                     /translation="VVLLPQKDYWDWVRACREFVLTFGAYLAQDPRAAGRYMSPRQVI
                     SFPRASGGYAESDDIEGWLQREYPEVRLDPVDAESPEGLEQALARRVADDDRFGQKQR
                     PFFLLWPTEFPVITQRFGANPRIYTRFGMPGHEGVDIRALPNTKVFACADGQVYRVEN
                     NPKAHAYGIHVRIRHRDGYRTIYGHLAKALVKEGEPVTGAQVIGLADATGASVGHHLH
                     LSLKKDQASELGETTYPLDIIDPTEFLVWPEPAWPKGLPNTAWPIGKCLVGAHGRVGA
                     ALEEADLQLIERARLEAIVVSLQDSAQTVERLRSHRPAMFLLARLTADFSGEPLTPIQ
                     YVASVQRRLRELYQAELRYVELCPNPNLQSEGWGRSWKDGQAFAAWFAEAASRFRDRF
                     PELKLGFPGLSPGEHVPGKKADPLQFLDAADEATLAADWVGVNCYWTDLRGMDALTGG
                     RMYEEYRLRYPAKLLIITEFGNPSIDLLSKSRAGQYLEFYRQVRDQAGIAAAFCFAIA
                     AGAGYPGLAWSAAGESDREISTVIGSRAF"
BASE COUNT          618 a         1138 c         1091 g          725 t
ORIGIN      
        1 tggcacacac cctcggattc atggtcctgc tcgtgctcat cctctacgtg aacgtccagg
       61 atttcctcaa ccccatcatc ctgccccgct aatcgttcgc catgcaaagt ggcttcgttg
      121 gattgcttga gttgctgggc aacgaagcct acccgctcga gccggccagc ctgaccgagc
      181 tctccgatct cgactccgtg cgcctggctc aattccgctc ggcctgggaa cgattcggca
      241 cgaagcggcg ccacgccatt ctgctccagt tgggtgaact ggcaaactcc aagatcgagc
      301 tctctttcga agcggtcaac cgattcgctc tccaggattc cgacccgccc acgcgcgctc
      361 aagcaatcgg caacttgtgg gagtccgagg acccgcagct cgctaggcag ctgttagaca
      421 tgcttcgcgc cgatcgggta gcggaggtga ggaaggctgc cgccgatgct ttgggttcgt
      481 tcgtcctgtt ggctgagacc gaggatctcg cggagcagct gcgccttgag atcgagcggg
      541 gtctgttgaa ggcagtccgt tcggatccga gtaaggaagt acgggagggt tgtctacagt
      601 ctctcggatt ctcgacccgc gaggaagtcc ccgagctgat tgagatggcc tattcctctg
      661 gggaaggttc cagcttgcgc gcagccctgc gggcgatgtc gcgttctgcc gattcccatt
      721 gggccccctt ggtcgtagca aaactgcagg acccggagcc aggtacccgc ttcgaggcgg
      781 tgcaggcggc cggagagctg gacatccgag aagcctcgga ggagctggtc gacctgctgg
      841 aggatgtgga cgaccggatc cgcaaggcgg cgatctggtc cctcagccag gtgggaggct
      901 cgatcgccat cgccgccttg gagcagctgc aggagagcgc ctccgaatcc gaggcgcagc
      961 tgattcaaga cgccctcgat aacctggaat tcgaggacgg ggcgcgggaa ctcttcaggc
     1021 tgaacttcga cgacgaggat tccgcggctt gagcggtctg ctcagttcgt ttgcagacca
     1081 cattcttcca atcctcatca ccgcggcagc agggttcctc gcccacaaac tgcttgggct
     1141 taacccccga ccgctctctc aaatcgcctt ctacgtgctc tcgccagcac tggttttcga
     1201 cctcttgcta tcggccgaca ttcgtggcct gcaggtcgtt tccatgatgg cactggcgac
     1261 gattgtggtc ttatccatag gtggattcag ttggctgctc ggccgcgccc tcggtctcga
     1321 ccccaagctt gcctcagcgg tgctgcttgc cacagccttt atgaatgccg ggaactacgg
     1381 tctgtcgctc aaccagtttg tctacggtga atcggggctg gcctgggccg ggcttttctt
     1441 cgtggcctcg atgatgttga ccaattcgct cggggtatat ggcgcttcga gaggtcgacg
     1501 gactccgctg gccgccctgc gcgagcttgc caaggtcccg gcagtctatg cgatcccgct
     1561 cgcgttgacc gcccgggcct tcgacctgca cctcccgttg gcgcttagcc gtccgatcga
     1621 tctcctggga acagccacgg tcccgatctt gctgttagtc cttggcatgc agattggtgc
     1681 gggcggagcc tccggccacc gtggattgat tgcagtctgt gttggccttc ggctggtagt
     1741 cgcgccgttg gttgcctggc tggtcgcgcc aagcctcggg ttggacggcc tcgctcggca
     1801 agtgggagtg cttgagtcag ccatgccgac cgcggtcatg agcacaatcc tcgcgattga
     1861 atttgacgtc gagccaggct tcgtcacccg ggtcgtaatg ctgacgactt tgctcagccc
     1921 gctgacgttg actccgctga tcacgctgct cgccagctag aacgcccggc tcccgatcac
     1981 ggtcgaaatc tcgcggtcag attcccctgc agcactccag gccagccccg gatatcctgc
     2041 gccggcggcg atcgcaaaac agaacgcggc ggcgatcccg gcctggtctc ggacctggcg
     2101 atagaactcc aggtactggc cggccctcga tttggaaagc aggtcaatcg aggggttgcc
     2161 gaattccgtg atgataagca gcttagcggg gtaacgcagc cggtactcct cgtacattcg
     2221 tccgccggtg agcgcatcca tgccgcgcag gtcggtccag taacagttca cgcccaccca
     2281 atccgccgcc agggtcgcct catcggccgc gtcaaggaat tgcagcgggt cggccttttt
     2341 cccaggcacg tgctcacccg gcgagaggcc tggaaagccg agcttcagtt cggggaaacg
     2401 atccctgaag cggcttgccg cctctgcgaa ccaagcggcg aaagcctgcc cgtccttcca
     2461 acttctcccc catccttcac tttgtaagtt cgggttcggg cagagctcga cgtagcggag
     2521 ctccgcctga tagagctctc gcagccggcg ttggacggag gctacgtatt ggatcggggt
     2581 gagtggctca cccgagaaat ccgccgtgag gcgggccaag aggaacatcg ccggtcggtg
     2641 gcttcgcaac cgctcaacgg tctgtgcaga atcctggagc gagacaacga tggcctccag
     2701 ccgggcccgc tcgatcagct gcagatcggc ctcctcgagc gcagctccga cccggccatg
     2761 ggcaccgacc aggcacttcc cgatgggcca ggcggtattc ggaagtccct tcggccaggc
     2821 cggctcgggc caaaccaaga attcggtggg atcaatgatg tctaagggat aggttgtttc
     2881 gcctagctcg ctggcctggt cctttttcag cgagaggtgc agatgatgac cgaccgaggc
     2941 accggtggcg tcggccaggc cgataacctg cgccccagtc accggctctc cctctttcac
     3001 cagagccttg gccaggtgcc cgtagatcgt tcggtagcca tcccgatgcc ggatccgaac
     3061 gtggatccca taggcgtgcg cctttggatt gttttccacc cggtacactt gcccgtcggc
     3121 acaggcaaag acctttgtgt tcggcaatgc ccggatatcg accccttcat gccccggcat
     3181 gccgaaccga gtgtaaatcc gggggttcgc cccgaagcgc tgggtgatca ccgggaactc
     3241 ggtaggccag agtaggaaga agggccgctg cttctgaccg aaccggtcgt catctgccac
     3301 ccggcgggcg agtgcctgct ccagcccctc tggactctcg gcgtcaaccg gatccagccg
     3361 aacttcgggg tattcccgct gcagccagcc ttcgatatcg tcggattcag cgtacccacc
     3421 cgatgcccgc gggaaggaga taacttgtcg gggcgacatg tagcggcccg ccgcccgtgg
     3481 gtcttgagcc aggtaagcgc cgaaagtgag cacaaattcg cggcaagccc gcacccaatc
     3541 ccagtagtcc ttttggggaa gcaggacgac tt
//
LOCUS       DATDPQ010000395         5441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_773701, whole genome shotgun sequence.
ACCESSION   DATDPQ010000395 DATDPQ010000000
VERSION     DATDPQ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773701"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VI520_08805"
     CDS             complement(<1..247)
                     /locus_tag="VI520_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38018.1"
                     /translation="MFAIKAEVSDPRAETFAFGEQKTMYGGKRIARGDTIFVFASENE
                     GGPGLIASGIVTSADAIPRKRGIARQTPRVSITIMRTA"
     gene            complement(865..3108)
                     /locus_tag="VI520_08810"
     CDS             complement(865..3108)
                     /locus_tag="VI520_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38019.1"
                     /translation="MEIPYLPGKAVVGPSPLARFLPPLEEGTVALAIERFGMAGQLVL
                     DPFGASPRLALEAAQQGCSVLVAVNNPITRYVLRHTLQPFALDELQSALSRLSASAKD
                     GGRLEPFLLDLYQTLCSRCGRQVSVEYFVWDREENVPVLKFYVCPHCNHTIEEPTDAE
                     DRRRSLEHGAKGLPHATALEQLAPRGSPDREHAEAAVSVYPGRAIYAIMTLLNKLQQN
                     ELGERMEAARALLLLMLDQTNALWGYPEGRIRPLQLTASPRYLESNAWRALEQAIGQW
                     SGSASRVELVEWAGEASLRPGGVTLFEGPVRELSKGLPAGRVERILTVIPRPNQAYWT
                     LSALWAAWLWGREAADPVRAALRRRRYDWAWHADALHTTLRAMRPALSDKVQVVGLVP
                     DAEPGFLAAALAGFDGAGFRLTGRALRPAEGQAILTWEWEGGPGQLAAPAQLAQIMKQ
                     AAQHALAERGEPSSYELPHEAAWSELATRRMLARAQGSESRTPLGTIGEDFDQALADR
                     SVFEHLGKGAEPETGRYWLSAPAEVSLPLADRVEGLALGILREQDRISDMELDRRLCE
                     ALPGLLTPDRRLVMAVLRSYAISEGGGGIWSLRAEDRGEARQADRAEIQSLLAGLGNR
                     LGFEVEGSDPVRWADRTNEQRFSFWVDETARLGTAMRRQLEQGESGRGSGLEVVVIPG
                     GRAALVAEKSRRDPLLQAWLESAPRVIKFRHVRRLAEDTTLDRGNLMERLVLDPPGHE
                     DPQLPLL"
     gene            complement(3187..4446)
                     /locus_tag="VI520_08815"
     CDS             complement(3187..4446)
                     /locus_tag="VI520_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38020.1"
                     /translation="MTRLRQIGQWAALVLTLLASPLLLGGSAALTLNRSDQVRNFTRP
                     VEFDYVSWTGVALAEKLEYSGLRPAEYLSEPERKTLVLEYVELIGETARLRAELEAIL
                     GDPNLDDPQQAAKPVGLQLEETRSRLEDLRPLAESVLQENVAAVLADLGLGWGPALFP
                     PVSFRFSSLPSALVVSPREVIRQDANLQLVPELSLEQQIDLESRVEQGLDVSALVVGI
                     GGLSTYPTMVMETTAMDWLVETIVHEWVHHYLAFRPLGLGYNRSPELRTFNETVAGLV
                     GREIGREVLARYYPEHLPPAPPQNPPAPLPGAPPEFDFQIEMRETRERVDALLAEGQV
                     EEAESYMEARRRVFWEHGYRHLRRINQAYFAFYGAYADVPGGPAGEDPVGDAVRQLWE
                     QVGDPVAFLRQVAWMSSPEDLFASPPG"
     gene            4584..>5441
                     /locus_tag="VI520_08820"
     CDS             4584..>5441
                     /locus_tag="VI520_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLF38021.1"
                     /translation="MNFLVTGAAGFLGSALANRLVREGHQVRGLDDLSAGDPGRLDPD
                     VLFTRGDVTDRPKLWSLLQDVDCVYHLAARVSVPESVLYPREYNAVNVGGTVSVMEAM
                     RDVGVKRVVLASSGAVYGDRQEQPLSEDTPPDPRSPYAVSKLAAEFYVRTIGSLWGIE
                     TVVLRVFNAYGPDQPLPAAHPPVIPNFLRRTVRGGTLVIHGKGEQTRDFVFITDVVEA
                     LVSAATAPNIGRSVINIGSGIETSIRDLAQQVLETVGTSAEWLYKEDQDPGTSRMCAD
                     IKRARVKLGY"
BASE COUNT         1011 a         1785 c         1681 g          964 t
ORIGIN      
        1 gcgcggtgcg catgatggtg atgctgacgc gcggcgtttg cctggctatt ccgcgtttcc
       61 ttggaatcgc gtcggcagag gtcacgatgc cgctagcgat tagacctgga ccgccctcgt
      121 tttcgcttgc gaagacaaag atcgtgtcgc ccctggcgat gcgcttgcca ccgtacatcg
      181 tcttttgctc gccgaacgcg aacgtctccg ccctcgggtc actcacctcg gctttgatcg
      241 cgaacatcgt agtcctcttc ccaggaattc ctcaaccgct cgtgacgcct aactagtact
      301 tagaccacat ccacccagta tagccctgct cccaagagtc ttatccggtg aaatccggca
      361 atatcacccc cgcaggggat gtgtagagga tggatttgca ctaatatggc ggcacagggt
      421 gtttatgcaa tgtggggtag gaccgagcgc gacgcagggt agccggtcgt gccgtaatat
      481 ggtcatagta gtagccgaag ctcattcagc gtgacgtgcg cgacacttgc gtctcagtca
      541 atggtgtaat ctctgtgtga tacctcagct tcaacgagtc gcatgttagc tcctcggtgc
      601 actggcgcca gccggaccag cagaaacacc ggccacagcc atgcaaaccg atttcaagac
      661 aatcatggaa accaccccag atcatcccct gactaagacc ttagaagatc gcggctgaga
      721 cccaattaga actagaacgt gacagaatca ctgaacgaag agagttactc tcgagctcat
      781 acgcggttat gagaatcagt attccatctt gggagctatt ggtcttgcgc aacggccaga
      841 ctaatcggag gatgcagtct ggttctacag cagcgggagc tgcggatcct cgtgaccggg
      901 cggatcgaga acgagccgtt ccatgaggtt gccgcggtcc aaggttgtat cttcagcaag
      961 gcggcgaacg tgacggaatt tgatgacccg cggcgcactc tccagccagg cctgcagcag
     1021 cgggtcgcgc cgggatttct ccgctacaag ggcggctcgc cctccgggga tcacgacaac
     1081 ttccagcccc gagccgcgcc cggactcgcc ctgctcaagc tggcggcgca tcgcggtgcc
     1141 cagccttgca gtttcatcca cccaaaagct gaaacgctgc tcgttcgtcc ggtcggccca
     1201 gcggaccggg tcgctgccct ccacctcgaa ccctaaccgg ttccccagcc cggccagcaa
     1261 ggattgaatc tccgcccgat cggcctggcg ggcctcgcct cgatcctccg cccgcaggct
     1321 ccaaataccg cctccgccct cggagatggc gtaggatctc aggaccgcca tcaccaatct
     1381 gcgatccggg gtgagcagcc caggcagcgc ttcgcacagc cgccggtcga gctccatatc
     1441 ggaaatacga tcctgttcgc gcaggatgcc gagagccaaa ccctccactc gatccgccag
     1501 tgggaggctg acctcggcgg gagcactcaa ccaataccgg ccggtctccg gttcggcccc
     1561 cttcccaaga tgttcaaaga ccgaccgatc ggcgagtgcc tggtcgaagt cctcgccaat
     1621 cgtccccaat ggagtgcggc tttcactccc ctgagctctg gccagcatgc gccgagttgc
     1681 aagttccgac caggccgcct cgtggggcag ctcatagctc gaaggttcgc cgcgttcggc
     1741 gagcgcgtgc tgcgccgcct gtttcatgat ttgggccagt tgggccggcg cggcgagctg
     1801 gccgggtccc ccctcccact cccaggtgag gatcgcctgg ccctcggccg ggcgcaaagc
     1861 ccgcccggtt agtcgaaatc ccgccccgtc gaagccggcc agggcagccg ccagaaatcc
     1921 tggttcggcg tcgggcacca agcccaccac ctggacttta tcgctgagcg caggacgcat
     1981 cgcccgcaag gtcgtatgaa gcgcgtccgc gtgccaggcc cagtcgtagc gtcgccggcg
     2041 cagcgcggcc cgcaccgggt cggccgcctc ccgcccccac agccaggcgg cccacaacgc
     2101 ggagagggtc cagtaagcct gattgggacg cgggatcacc gtgaggatcc gttcgacccg
     2161 tccggcaggc agccccttcg agagttcgcg caccggcccc tcgaagagcg tgaccccacc
     2221 cggccgaagg ctcgcctcgc ctgcccactc gaccagttcg acccgggatg cgctccccga
     2281 ccactgcccg atagcctgct ccagcgcccg ccaggcgttc gattccaaat aacgcggaga
     2341 agcggtcagc tgcagcggac ggatgcgtcc ctccggatac ccccagaggg cattggtctg
     2401 gtcgagcatc agcagcaaca aggcccgggc cgcttccatc cgttctccca gctcgttctg
     2461 ttgcagcttg ttaagcaggg tcatgatggc gtagatcgcc cgccccgggt acacactcac
     2521 ggcagcttcg gcatgctccc gatccgggct gccgcgcggt gcaagctgct cgagggcagt
     2581 ggcatgcggc agccctttcg ctccatgctc tagcgagcgg cgtcggtctt ccgcgtccgt
     2641 cggttcttcg atggtgtgat tgcagtgagg acagacatag aacttcaaga ccggcacatt
     2701 ctcttctcgg tcccagacaa aatactcgac ggagacctgc cggccgcagc gggagcagag
     2761 agtctggtac agatccagca gaaacggctc tagccgaccc ccatccttgg ccgaagcgga
     2821 cagccgggag agggctgact gcaactcgtc cagcgcgaag ggttggaggg tgtggcggag
     2881 cacgtagcgg gtgatcgggt tattcaccgc caccagcacc gaacatccct gttgagcggc
     2941 ctccagcgcg agccttggcg aggcgccgaa ggggtcgagg accagttgac cggccatccc
     3001 gaagcgttcg atggccagag ccacggtgcc ctcttccagc ggcggcagaa accgggccaa
     3061 ggggctggga ccaaccaccg ctttcccggg gaggtaaggg atctccacgg ttcgagtata
     3121 agggacccga tccggttgtc ggaagagttc gggggccgcg gacctggccg tcgaccggtt
     3181 aggttttcaa ccggggggcg aggcgaagag gtcttcgggg ctgctcatcc aggcgacctg
     3241 acggaggaag gcgactggat ctccgacctg ctcccagagc tggcgaaccg catcgccgac
     3301 cggatcttcg ccggccgggc cgccgggcac gtcggcatac gcaccgtaga acgcaaaata
     3361 agcctggttg atccgccgga ggtggcggta gccgtgctcc cagaataccc ggcgccgggc
     3421 ctccatatag gattccgctt cctcaacctg cccctccgcg agcagcgcat ccacccgctc
     3481 acgggtctct ctcatctcaa tctgaaagtc aaactcaggc ggcgctccgg gcagcggagc
     3541 tggagggttc tgcggcggag caggaggcag atgttcggga tagtagcggg ccagcacttc
     3601 tcttccgatc tccctaccga ccaggccggc gacggtttcg ttgaaggtgc gcagctcagg
     3661 ggagcggttg tagccgaggc cgagcggacg gaacgccaga tagtgatgca cccattcgtg
     3721 gacgatggtc tcgaccagcc aatccatggc agttgtctcc attaccatcg ttggataagt
     3781 ggagaggccc ccgatcccga ccaccagggc cgagacgtcg agcccctgct cgacccgaga
     3841 ttccagatcg atctgctgct ccaggctgag ctcgggcacc aattggagat tcgcatcctg
     3901 gcggatcacc tcccgggggg aaaccaccag tgccgatggc aggctcgaaa agcggaagga
     3961 gaccgggggg aacaaggccg gcccccaacc gagtccgagg tcggccagga ccgcggccac
     4021 gttttcctgc agtacggatt cggccaaggg ccgaagatct tccagccggc tgcgggtttc
     4081 ctcaagctgc agcccgaccg gcttggccgc ctgttgcggg tcgtccagat taggatcgcc
     4141 gaggattgcc tccagctcgg ctcgaagccg ggcggtctcg ccgatcagct ccacatactc
     4201 caagaccagg gtcttgcgtt cgggctcgct caggtattcg gccggccgca gcccggagta
     4261 ctcgagcttc tccgctaatg ccacccccgt ccaactgacg taatcaaact ccacgggacg
     4321 ggtgaagttt cgcacctggt cgctgcggtt cagggtaagc gccgcgctcc ctccgagcag
     4381 gagcggcgag gccagcaagg tcagcaccag ggccgcccac tgtccgatct gcctgagcct
     4441 ggtcatttgc gcatcttatc aggaactgat cgggtgagct gcggacgacc gccccctgaa
     4501 tcggccggga aagtcgctgt gctataatcc gccggcgcgg gacccgctgg gtcccgtgcc
     4561 gacaattagc cctatcccgc caaatgaatt tcctcgtcac tggcgccgca ggcttcctcg
     4621 gatccgcgct ggccaatcga cttgtcaggg aagggcacca ggtccggggc ctggatgacc
     4681 tctcggccgg cgatccgggg cggctcgacc cggatgtgct cttcacccgc ggcgacgtaa
     4741 ccgaccgccc caagctctgg tccttgctgc aagacgttga ctgcgtgtac cacttggccg
     4801 cccgggtttc ggtgccggaa tcggttctgt acccccgcga atacaacgcg gtaaatgtcg
     4861 gggggacggt cagtgtcatg gaagcgatgc gagatgtggg tgtcaagcgg gtggtgttgg
     4921 cctcctccgg cgccgtgtac ggagaccggc aggaacaacc cctgagcgag gacacgcctc
     4981 ctgatcctcg ctccccctac gcggtttcca agctggccgc ggagttctat gtgcgcacga
     5041 tcggatcctt gtgggggatc gaaaccgtgg tgctgagggt gttcaacgcc tatggccccg
     5101 accaaccgct gccggctgcc caccctccag taatcccgaa cttcctgcgc cgcacggtgc
     5161 gaggtggaac gctggtcatc catgggaagg gcgagcagac ccgggacttc gttttcatca
     5221 ccgacgtcgt cgaagcgctg gtgtcggctg cgaccgcgcc gaacatcggt cgttcggtga
     5281 tcaatatcgg aagcggaata gagaccagca ttcgcgacct cgcccagcag gtgctggaaa
     5341 ccgttgggac cagcgcggag tggttgtaca aagaagatca agaccctggg acctctcgga
     5401 tgtgtgccga catcaagcgg gcccgagtca agctgggcta c
//
LOCUS       DATDPQ010000396         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_515984, whole genome shotgun sequence.
ACCESSION   DATDPQ010000396 DATDPQ010000000
VERSION     DATDPQ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515984"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VI520_08825"
     CDS             <1..202
                     /locus_tag="VI520_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38022.1"
                     /translation="TQGRGPVADWHRNQQAYETFRDLPHDLREFLGKRDSPRLIRLAI
                     RLEELSIDRLRNLGELISDIAR"
     gene            223..726
                     /locus_tag="VI520_08830"
     CDS             223..726
                     /locus_tag="VI520_08830"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLF38023.1"
                     /translation="MSQALEAYHQGKLEDAIRLFESAHGLEADPARRAELANNLSVSY
                     LKFGRSQEALSIVRGTGELFSKLGDIQRAAQATGNLAAALEATGNLPAAETAYQQAAE
                     SFRALGDQDSLGYTLNALAQVQLRQHRPLEAAITLQAGALASPPRGLRGRLLKRLLRL
                     PSRLLRF"
     gene            738..1583
                     /locus_tag="VI520_08835"
     CDS             738..1583
                     /locus_tag="VI520_08835"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLF38024.1"
                     /translation="MSGWTIRQTTADDRALVDGLVLGSRWKHLHLDWLEPAALTDQSP
                     FLLAMQAGVPVGCLGCPPDLPGVSWLRAFAVTSGVKPEAAWQPLWELAASALRELRVD
                     RVAALPIEGWFESLLAGAGYQPANAVIFFERELTRSIPSPSLRSRPIQGEDERALLEL
                     DHLAFEPLWQLSSDSLRAALIQSDSATIIESAGGLAGYQITTRSPLGAHLARLAVHPG
                     HRRRGLGRSLVLEALQSARQGGLSRLSVNTQADNLASQRLYRSLGFRETGQHYRVLET
                     RLPKL"
     gene            1641..2243
                     /locus_tag="VI520_08840"
     CDS             1641..2243
                     /locus_tag="VI520_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HLF38025.1"
                     /translation="MRLPPDRSLPNYRMRWLVMAIEEQMGAHGLRMILRQSDLTRYLE
                     SLPPANDELQSRSADYARLLTTVRTYFGLGARGTLTRIGRSIFRNQVAQRPLLNARRK
                     IIGPFLSTQSKLAWILNQLASEMAAPNGQVRVSRAGEGFWLVDETFDRTLGVVAEHPI
                     CWSTVGELAEAVTWMTREEVPIREVDCRASGKEACRFAVL"
BASE COUNT          393 a          743 c          703 g          439 t
ORIGIN      
        1 cacccagggc cgcggcccgg ttgccgactg gcatcgcaac cagcaggcct acgagacctt
       61 ccgcgacctt ccccacgacc tgcgcgagtt tctggggaag cgagatagtc cccggttgat
      121 ccggttggct attcgcctcg aggaactttc tatcgatcgg ctgcgcaatc tcggcgagct
      181 aatctccgat atcgcccgtt gaatgccgaa ccctctagcc ttgtgagtca ggccctcgaa
      241 gcctatcatc aggggaaact ggaggacgcg attcgcctct tcgagtcggc ccatgggttg
      301 gaggccgatc cggccaggcg ggccgagctg gccaacaacc taagcgttag ctacttgaag
      361 ttcgggcgct cacaggaagc cctctccata gtccgaggta cgggggagct tttctccaag
      421 ctgggcgaca tccaacgggc tgcccaagcg acgggcaacc tggccgccgc cctggaggcc
      481 actggcaatc ttccagccgc cgagactgcc tatcagcagg ctgccgagtc gttccgtgct
      541 ctgggggatc aagactcgct tggatacacg cttaacgccc ttgcccaggt tcaattgcgc
      601 caacatcggc cgctcgaggc ggccatcact ttgcaagccg gagcccttgc aagccctccc
      661 cgcggcctgc gcggccggtt gctcaagcga cttcttcgac tgccctcccg cctgcttcga
      721 ttctgaaccg cggtccgatg agtggctgga ccattcgtca aactacggca gatgaccgag
      781 cgttggtgga cgggttagtc ctcggttccc gctggaagca cctgcatctg gattggctgg
      841 agccagccgc cctaactgac caatctcctt ttctgctggc aatgcaggcg ggcgttccgg
      901 taggctgtct cggttgtccg ccggacctgc ccggtgtgag ctggctgcgg gcgtttgcag
      961 tgacgtccgg agtcaagcct gaggcggcct ggcagccgct ctgggagctg gcggccagcg
     1021 ccctgcgcga actgcgggtg gaccgagtgg ctgcgctgcc gattgagggc tggttcgaat
     1081 cgctgctggc cggggctggg tatcagcccg ctaacgcggt gatcttcttc gagcgtgaac
     1141 tgacccgctc gatcccatcc ccttctctcc gatccagacc catccagggc gaggatgaaa
     1201 gggcactgct cgaactcgat cacctcgcct tcgagccgct ttggcagctc tcgagcgatt
     1261 cgcttcgggc tgcgctcata cagtcggatt cggccacaat catcgagtcg gcgggtgggc
     1321 tggccggcta ccagattaca acccggtcac cacttggagc ccacctggcc cgattggcag
     1381 tgcaccccgg ccatcgccga cgggggctgg ggcgttcgct cgtcttagag gccttgcaga
     1441 gcgcccgcca gggtggtctc agtcggctct cggtaaacac ccaggccgac aacctggcca
     1501 gccagcgcct gtatcgcagt cttggattcc gggagaccgg ccagcattac cgagtccttg
     1561 agacccgcct cccaaagcta taatccgcta tggattcctg gtccggctcc gcggatcggc
     1621 cacaagcatt gcccctcgag atgagattgc ctcccgatcg ttccctaccg aactaccgga
     1681 tgcgctggtt ggtcatggcg attgaggaac aaatgggggc ccacggactt cggatgatcc
     1741 tccgtcaatc cgacctgacc cgatatctgg aatccctgcc tccggccaac gacgaactcc
     1801 agagccgctc ggcggactac gctcgattgc tgacgaccgt tcggacttac ttcgggctcg
     1861 gtgcccgcgg gacgctgacc cgcatcggtc gatcgatctt ccgcaaccag gtcgcgcagc
     1921 gccccctgct caatgcccgc cgaaagatca ttgggccctt tctatcaacc cagagcaagt
     1981 tggcctggat tctgaaccag ctggcgagtg agatggcggc cccgaatggg caagtccgag
     2041 tcagccgggc cggcgagggg ttctggcttg tcgacgagac cttcgaccgc accctagggg
     2101 tggtcgccga gcacccgatc tgttggtcga cggtcggcga gttagcggaa gcggtaacct
     2161 ggatgacacg ggaggaagtc ccgatccgcg aagtggactg ccgggcaagt ggtaaagaag
     2221 cgtgccgctt cgcggtcctc taaggttcgg gcttagccag ggtcggggat tccacacc
//
LOCUS       DATDPQ010000397         2465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_1045944, whole genome shotgun sequence.
ACCESSION   DATDPQ010000397 DATDPQ010000000
VERSION     DATDPQ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045944"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VI520_08845"
     CDS             complement(<1..220)
                     /locus_tag="VI520_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38026.1"
                     /translation="MFGPFGPFGIRFDWPEFFVGLGTGLVFALALWRLRAVFGWGLQM
                     LRDALGSLRTGLTSKAIDRYQVELISRAE"
     gene            complement(223..924)
                     /locus_tag="VI520_08850"
     CDS             complement(223..924)
                     /locus_tag="VI520_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38027.1"
                     /translation="MSRASGLHRLQAMDLAADRIRVRQQEIEAQLADSAELERLRTDL
                     AAASEAAQAAGAVVRTAHRVVQDQRHKLKETEQALYSGRSQTPKELAELQAEAESLKR
                     HLDTLEDRYLEAMLSQDQVAAEERQSQLAVNSLMSDRSTLHSELQAELGQIRDRLNDF
                     TIEREAALVSVSEPDLATYERLRSRLGGIAVATLEEASCGACGLRVSASREQVVRAGG
                     ELVTCGQCGRVLYGG"
     gene            998..>2465
                     /locus_tag="VI520_08855"
     CDS             998..>2465
                     /locus_tag="VI520_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38028.1"
                     /translation="MFGPRSAAGVSAVALLLVNLPYIVAPSLIAPQSSFSGFLLNPID
                     GHSYLAKIRQGAAEPLEFRLPYAAEPGDGALLFVYYLVLGRVGSRVGLAPLTMVHSAR
                     VLGAAAMFAATYLFVKRILAPGRAQWAAFGLALFGTGIGWLAVPFGLTPVDLQVPEAI
                     PFMTAFVNAHFPAAAALLLFSVTAIASGYPALLIYLASLGLSLIQPFGSLTVGAALGA
                     WVLVEAIVRRKSDRGPASDSGSEPRRPRRTGVAAFLLGALPVLVYDFGVIRTHPVLGG
                     WAAQNITPSPSLPETLLGYGLLVPLAVIGIRAARLRERSESRLLVSWVLVNGLLLYGP
                     FLLQRRLSLGLFFPLAALAGLGLERLASSRRRFIFLLVGTLALSLPSHGLVIASGLSS
                     VARGDPALVVDRDDEELTQWIGNHLIDSELILAGPRTGNRLPAFTPARVIYGHPFETP
                     KAAEWRAELVRLFGWDQDPGSGLERLRNLGVRYVLYSSE"
BASE COUNT          392 a          815 c          758 g          500 t
ORIGIN      
        1 tctccgcccg ggagatgagt tccacctgat atcggtcgat cgctttagag gtcagaccag
       61 tacggagcga tccgagcgcg tctcggagca tctggagccc ccagccgaac actgcccgca
      121 atcgccacag tgcgagggca aacaccaggc cggtcccgag accgacaaag aactcgggcc
      181 aatcaaatcg aattccgaag gggccaaagg gtccaaacat ttctatccgc cgtacagcac
      241 gcggccacac tgaccgcagg taaccaattc acctccagcc cggaccacct gttctctcga
      301 agccgagacc cggagaccgc atgctccaca gctagcctct tctagggtgg cgaccgcgat
      361 gcctcccaac cgggagcgca gcctctcgta ggtcgccagg tctggctctg agacgctgac
      421 cagcgcggct tcgcgctcga tcgtgaagtc attcagccgg tctcgaattt gcccgagttc
      481 agcctgcaat tcggagtgaa gtgtggaacg gtccgacatc aacgaattga ccgccaactg
      541 tgactggcgc tcctcggccg ccacctgatc ctgactcagc atcgcctcga gataccgatc
      601 ctccaacgtg tccaggtgac gtttgagcga ctccgcttcg gcttgaagct cggccagctc
      661 tttaggagtc tggctcctcc cactatacag ggcctgttcg gtctccttca gcttatgacg
      721 ttggtcctgc acgacgcgat gggcagttcg taccactgcc ccggccgcct gggccgcctc
      781 gctcgccgcg gccagatcgg ttcgcaggcg ctccagctcg gccgagtcgg cgagttgggc
      841 ttcgatttct tgctggcgaa ccctgatccg atctgcagcg aggtccatgg cttgcaggcg
      901 gtgaagaccg gaggcgcggc tcatttccgg attatagatg ctggcattcg aggagagcaa
      961 atggcgggcc cccctgaccc gacgtacaat cccacccgtg ttcgggccga gatccgcagc
     1021 cggagtaagc gcggtcgccc tgctgctggt caacctgccc tacatagtag ctcccagcct
     1081 catcgccccc caatccagct tcagcggatt cttgctcaat ccgatcgacg gccacagtta
     1141 cctggccaag atccggcagg gagccgcgga gccgctggag ttccggctgc cgtatgccgc
     1201 cgagccggga gacggagcgc tactcttcgt gtactacctg gtcctcggcc gggtgggcag
     1261 ccgggtcggg ctggcgccat tgactatggt ccattccgcc cgtgtgttgg gggcggccgc
     1321 aatgttcgcc gccacctact tgttcgtcaa acggattctc gcaccgggca gagcccaatg
     1381 ggcggccttc ggtttggcgc tcttcgggac cgggattggg tggctggccg ttccctttgg
     1441 ccttacgccg gtggatctcc aagtacccga ggccatccct ttcatgacgg cgtttgtgaa
     1501 cgcccacttc ccggccgccg ccgccttgct gctcttctcg gtgaccgcta tcgcctccgg
     1561 ttacccggcc ttgctgatct atctggcaag tctcgggctg tcgctgattc agccttttgg
     1621 ctcgttgacc gtgggggcgg ccctcggagc ctgggttctg gtcgaagcga tcgtgcggcg
     1681 caaatctgac cgcggcccgg cctccgactc gggttccgaa cccaggcgac cgcggcggac
     1741 gggggtggcg gcgttcttgc tcggcgcgct cccggtgttg gtctacgact tcggggtcat
     1801 tcgcacacac cccgtactcg ggggatgggc cgcccaaaac attacacctt ctccctcact
     1861 gcctgagacc ctgttgggat atggactgct cgtgccgctg gcggtgatcg ggatcagggc
     1921 cgccaggctg cgcgaacgga gtgaatctcg attgctcgtc agctgggtgc tggtcaacgg
     1981 tctgctgctg tacggaccat ttctgctgca gcgccggctc agcctggggc tcttctttcc
     2041 gttagcggct ctggccggcc tgggactcga gcgactggcc tccagccgcc ggcgtttcat
     2101 cttcttgctg gttgggacct tggccctctc ccttccctcc catggcctgg tcatcgcctc
     2161 ggggctaagc tcggtggcgc ggggagaccc tgcgctggtc gttgaccgag atgatgaaga
     2221 gttgacccag tggatcggaa accatctgat cgattcggag ctgatcctgg ccggaccccg
     2281 aaccggcaac cgcctcccgg cattcactcc ggcacgcgtg atctacggac atccgtttga
     2341 aactcccaag gctgccgagt ggagggcaga gctggtccgg ttgttcggct gggaccagga
     2401 cccgggaagc ggtctcgagc ggctccggaa cctgggcgtc cggtatgtgc tctactcgag
     2461 cgagg
//
LOCUS       DATDPQ010000398         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3656123, whole genome shotgun sequence.
ACCESSION   DATDPQ010000398 DATDPQ010000000
VERSION     DATDPQ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656123"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VI520_08860"
     CDS             <1..274
                     /locus_tag="VI520_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38029.1"
                     /translation="YSLRAWDGEAMTNLVRATTGTSIQAGSEQVNRVGLLATGDQLIL
                     YVNGARLTEIEDSRFSEEGSFGVFVGARQTSNFTIRVSEIAYWESP"
     gene            288..905
                     /locus_tag="VI520_08865"
     CDS             288..905
                     /locus_tag="VI520_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HLF38030.1"
                     /translation="MSERFISRDFVGKRREAAAGDRVPPGQHPVDDFPVLSAGPTPRT
                     DLDKWSFSLEGELAKPARWSWEEFLALPAQEFTVDIHCVTKWTKLDTRWKGVSVDTLL
                     EQVELSPKAGYVTAFSDGGYTTNLPLPDVINGQAFIGYEYDGRPLAPEHGGPARLVVP
                     HLYFWKSAKWVRGLRLMEKDKRGFWESLGYNNHGDPWKEERYVGD"
     gene            912..1658
                     /locus_tag="VI520_08870"
     CDS             912..1658
                     /locus_tag="VI520_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HLF38031.1"
                     /translation="MKRLLEWQLATVKSIREETYRTKTLVLSLPAWVAHRPGQHYDVR
                     LTAPDGYQAQRSYSIASEPERTGEIDLTVERLAEGEVSTYLHDVLAPGDRIEVRGPIG
                     GYFVWEASLGGPLLLVAGGSGVVPLMSMVRHRRAAGSTVATHLLYSSRTPEDVIYADE
                     LRGLQQSGNGLRVIQTFTRTQPPNWTGYARRIDVAMLKEVAGPLGRRAQAFICGPTLL
                     VEAAADGLVEAGLSPERIKTERFGPSGGAG"
     gene            1673..1963
                     /locus_tag="VI520_08875"
     CDS             1673..1963
                     /locus_tag="VI520_08875"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HLF38032.1"
                     /translation="MGDATDGIRANMLDGNSAAGVLEEIFGEEMTASPTECDHCGREG
                     ELGSLLMFTQAPGLVLRCPACSEVMLRVVQTPDYTYLDVRGAVYLRLARRTA"
     gene            complement(2076..3293)
                     /locus_tag="VI520_08880"
     CDS             complement(2076..3293)
                     /locus_tag="VI520_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HLF38033.1"
                     /translation="MPVLPRFDQFEGRHWETGSVSNALAYAGARVPQTKQRYSEALLL
                     GISGGAAFGYFVFDYKGHDPHVALLSRNTFDPLQTLLERLAIPQDLFQTSDPKKGERN
                     LIEVLESGRPAIVWADAFSLPYNSPAADQAIWGMLPILVYGHEDGKAYIADRSAKPLT
                     STADELARARARVKKDKFRVLALGAPDPKKLPGAIQKGIWQCIELFTEPPPKGTRDNF
                     GFAAYQKWANMLTNTRNPQSWERLLAPGSRMYAALAGSEHQPGAFGWARTFPSNQVDD
                     RKLYADFLDEAALILRKPKLKPAGEKFRASSAAWEELAEALLPDEVPLFKETRQLLLR
                     KRDLFINKGEAALEERKVISARLAEIRANIDKKFPLSAEEVAGLRGRLAEQVLNVSEI
                     ERQAIELMQAAMA"
     gene            3342..>3931
                     /locus_tag="VI520_08885"
     CDS             3342..>3931
                     /locus_tag="VI520_08885"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HLF38034.1"
                     /translation="MPGEQDRDIVWTFARRRRLGGAAAAVLILVTLLAVRRPIERAQP
                     AEPSASNPTPESAARGGSLSVTLVVTPAPRSSTSASTATPAPPTTETVPAVGPAYRPG
                     FNPLTGLPVSDPQTLLTVPLLVSITEFPPSARPQSGLSAASQVWETAIGEGMSRFLAV
                     YYGDYQEVLSDLLESHPKLTTSDPAIGPIRSGRVGY"
BASE COUNT          663 a         1225 c         1307 g          736 t
ORIGIN      
        1 ctactcactg cgcgcttggg acggggaggc gatgaccaac ctggttcggg cgacgaccgg
       61 caccagcatc caggccggct ccgagcaggt caaccgagtc ggcttgttag cgaccggtga
      121 ccaactgatc ctctacgtaa atggggcgcg gctcaccgag atcgaggatt ccaggttctc
      181 cgaggaaggc tcttttggtg tgttcgtcgg cgcccgccag acctccaact tcacgattcg
      241 cgttagcgag atcgcttatt gggagagccc ttaggccgag tcgggacatg agcgagcgct
      301 tcatttccag agatttcgtt ggtaagcgga gggaagccgc ggcaggggat cgggtgccgc
      361 caggccagca cccggtggac gacttcccgg tgctttccgc cggacccact ccgcggaccg
      421 atcttgacaa gtggtccttc agcctcgagg gcgagctggc caagccggct cgttggtctt
      481 gggaagaatt cctggcgctg ccggcgcaag agttcaccgt cgacattcac tgcgtgacca
      541 agtggacgaa actcgatacc cgttggaaag gggtgagcgt cgacaccctc ttggagcaag
      601 tcgagctgag ccccaaggcc gggtacgtta cggccttcag tgacggaggc tatacgacaa
      661 acctacccct gccggatgtg attaatggcc aggcgttcat cggctatgaa tacgacggcc
      721 ggccgctggc gccggagcac ggcggcccgg cccggctcgt cgtgccgcac ttgtatttct
      781 ggaagagcgc caagtgggtc cgcgggctgc gcctgatgga aaaggacaaa cgcggtttct
      841 gggagtcgtt gggctacaac aaccacgggg atccctggaa ggaagagcgc tatgtcgggg
      901 attgagcgct catgaaacga ctgctcgagt ggcagctcgc gacggttaag tcaatccggg
      961 aggagactta ccggacgaag accctggttc tctccctgcc cgcctgggtc gcccaccggc
     1021 cgggtcagca ttacgatgtc cgcctgaccg cgccggacgg ctatcaggcc cagcggagct
     1081 actccatcgc ttccgagccg gagcggaccg gcgagatcga cctcaccgtc gagcggctgg
     1141 cggagggcga ggtctccacc tatcttcacg atgtgttggc ccccggcgac cggatcgagg
     1201 tgcgaggccc gatcggaggt tactttgtct gggaggccag cctgggcggc cctttgctgc
     1261 ttgttgccgg cggatcggga gtggtgccgt tgatgtcaat ggtccgccat cggcgggcgg
     1321 ccggttcgac cgtcgccacc catctgctct acagttcccg gactccggaa gacgtgattt
     1381 atgccgatga gctccgggga ttgcagcagt ccggaaatgg gttgcgagtc atccagacct
     1441 ttaccagaac ccaaccgccg aattggaccg gttacgcccg gcggatcgat gtggcgatgc
     1501 tcaaagaggt cgccggcccg ctgggtcgcc gggcccaggc gttcatctgc ggaccgaccc
     1561 tgctggtgga ggcggcggcg gacggcctgg tcgaggccgg cctgtcgccg gagcgaatca
     1621 agaccgagcg tttcggtccc agcggaggag caggctgagg aggagtccgg ctatgggcga
     1681 cgccacggat ggaatccgcg cgaacatgct cgatgggaac tcggcggcgg gtgtgctcga
     1741 agagatattt ggcgaggaga tgactgccag cccgaccgag tgcgatcact gtggccggga
     1801 gggcgagttg ggcagcctgc tcatgtttac ccaagccccg ggtctcgtgt tgcgctgccc
     1861 ggcctgcagc gaagtgatgc tgcgggtcgt gcagacgcca gattacacgt atttggatgt
     1921 ccgcggggcg gtgtacttga ggctggcgcg ccggacagcc tgagcccagg acagatccgg
     1981 cgtggtctcg atgtcgcgtg agtgccgagg gcaatcagcg gcggggtagc ggcccaggct
     2041 ccgagctggg ggtgaggcta gctaagctgc ccgcttcagg ccatcgcggc ctgcatcaat
     2101 tcaatcgcct ggcgttcgat ctcgctgaca ttcaagacct gctcggccag gcgcccccgc
     2161 agcccggcca cctcctcggc ggatagtggg aacttcttgt cgatgttggc ccgaatctcg
     2221 gccagccggg cgctgatcac ttttctctcc tccagcgccg cctcgccctt attgatgaag
     2281 agatcccgct tgcgcagcag cagctgccgg gtctctttga acaacggcac ctcatcgggc
     2341 agcagcgctt ctgccaactc ctcccaggcg gcgctgctcg cccggaactt ctcgcccgcc
     2401 ggcttgagtt ttggcttgcg caggatcaaa gcggcctcgt ccaagaagtc ggcgtagagt
     2461 ttgcggtcgt ccacctgatt ggaggggaac gtgcgggccc agccgaaggc gcccggctga
     2521 tgctcgctcc cggccagggc cgcatacatg cggctgcccg gagccaacag ccgctcccaa
     2581 ctctgcgggt tacgggtgtt ggtcagcatg ttggcccact tctgataggc ggcgaaaccg
     2641 aagttgtctc gggttccctt cggcggtggc tcggtgaata gctcgatgca ctgccagatg
     2701 cccttctgga tggcgccggg cagctttttc gggtcgggcg cgccgagcgc cagcactcgg
     2761 aacttgtctt ttttcactcg ggccctggcc cgcgccaatt catccgcggt ggaggtgagc
     2821 ggtttggccg aacgatcggc gatgtaggcc ttcccatctt cgtggccata aacgagaatt
     2881 ggcagcatcc cccagatggc ttggtcggcg gcgggggagt tgtagggcaa actgaaggcg
     2941 tccgcccaca cgatcgccgg gcggccgctc tccaacacct cgatcaggtt tcgctcaccc
     3001 ttcttgggat cgctggtctg gaagaggtct tgcgggatcg ccagccgttc cagcagggtc
     3061 tgcagcgggt caaaggtgtt gcgggagagc agcgccacat gcgggtcgtg acccttgtaa
     3121 tcgaacacaa agtacccgaa ggccgcgccg ccgctgatcc cgagcagcag agcctccgaa
     3181 tagcgctgtt tggtctgcgg cacccgggcc ccggcgtatg ctaaggcatt gctcaccgag
     3241 ccggtctccc agtggcgtcc ttcgaattgg tcgaatcggg ggagcacagg catgtccggt
     3301 ttcctttcag gtaatatctg cggggattat agagaaattc catgcccggt gagcaagatc
     3361 gagacatagt ctggactttt gccaggcgac ggcggctggg cggcgcggcg gccgccgtgc
     3421 tgattctcgt taccctgctc gctgtgaggc ggcctattga acgagcgcag ccagccgagc
     3481 cgtcggctag taacccgaca cctgagtcgg ctgcccgtgg gggatcgctg tcagtcaccc
     3541 tggtggttac cccagccccg agaagctcga ccagcgcttc gaccgcaacg cccgctcccc
     3601 ccacaaccga gacggtgccc gcggtgggcc ccgcctaccg gcccggcttc aacccgctga
     3661 ccgggctgcc ggtttccgac ccccaaactc tgctcacggt tccgctgctg gtctcgatta
     3721 ccgagttccc gccctcggct cgcccacagt ccggactctc cgccgcctcg caggtctggg
     3781 aaacggccat cggggagggg atgagccggt tcctggcggt gtattacgga gactaccagg
     3841 aggtcttgtc cgacctgctc gagagccacc ccaagttgac gacgagtgac ccggctatcg
     3901 ggccgatccg ttcggggagg gtcggctatc a
//
LOCUS       DATDPQ010000399         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U7925
            k141_3138689, whole genome shotgun sequence.
ACCESSION   DATDPQ010000399 DATDPQ010000000
VERSION     DATDPQ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962693
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 10:15:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,781
            CDSs (total)                      :: 1,751
            Genes (coding)                    :: 1,746
            CDSs (with protein)               :: 1,746
            Genes (RNA)                       :: 30
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138689"
                     /isolate="SMAG_U7925"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VI520_08890"
     CDS             <1..677
                     /locus_tag="VI520_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38035.1"
                     /translation="GLVGAELGEAIIGDLQELRPTVEAHWGKGGSWLWLALEVVRGAA
                     VLTAQRLSPASGRRKENRSMDNWKETVRRAAAAIGLIACLPAAVLVVGGLLQSDWSAP
                     AVRSALERTLFNPDLVGFRVLIHPATILSGLLLAAGLNLIPLLRIGLERRPGTLVGTL
                     ALRIRSAHLGVAVVALGLLGVILGYGFTENFRVVPTHVSVQVSGWQIERDLLMAVCEE
                     AQCGQR"
     gene            664..1512
                     /locus_tag="VI520_08895"
     CDS             664..1512
                     /locus_tag="VI520_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HLF38036.1"
                     /translation="MGSDDPLILAITSDYFLIPRLEDTARALGFRLEVVQTAADLGAE
                     GPPIPRAIPLTEPLEGPDAAFVRSLVDRRPALLLVDTAHDGVPWEHWIQTLKTGSATR
                     RIPVVAFGPHVEAEVLQRAIRAGAEEAVSLGQLQASLPQIIERRARRIDLAALKEACD
                     QPLSTLGAHGLGLLNSGEYFEAHEVLEKAWMEAPEWHGYLYRSLLQVSVAYLQVQRAN
                     YPGAVKMLLRLKQWLEPLPDRCSGIDIAALKRNMAEFSAAVEAAGVDGIATLDPGLLR
                     PVLSQE"
     gene            complement(1503..2678)
                     /locus_tag="VI520_08900"
     CDS             complement(1503..2678)
                     /locus_tag="VI520_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLF38037.1"
                     /translation="MAGQADGSVVIVGAGVFGASAALELRKRGHIVTLLDPGPVPHVL
                     AASTDISKVLRMDYGADEFYMELMEAAFQGWDAWNEGWGEPLFHQTGMLLLAGGPMAP
                     GGFEHDSYQLLRARGHHPERLSAAQIKERFPAWDPADHPEAYFNPRAGWAESGEVVAR
                     LIADGKKLGVKVYASAEMRTLIEQGKRVHGVRMADGREFEAEYVVLAAGAWSPALLPQ
                     LEDLMWSTGQPVFHFRVEDIESFQPPKFAVWSADIAGTGWYGFPALDDGTVKVANHGP
                     GRRQAAAEPRQVDPGYEEMFRAFFRRSLPALAEAPMISNRLCMYCDTWDGDFWIDFDP
                     DREGLLVAAGGSGHGFKFAPMLGPIIADVLERKPNRFAHRFRWRRRGELKTEDARYS"
     gene            complement(2709..>3772)
                     /locus_tag="VI520_08905"
     CDS             complement(2709..>3772)
                     /locus_tag="VI520_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLF38038.1"
                     /translation="LIHIAVDGRPLVEHPTSAAAQHLLEWINGLSGPETKFSLVHPDG
                     ELPELPPGVRSWPVGPIRRGWERLRYDQITLPRAVAQAGAELLLTFSDGIPLKARMPI
                     AVVPPIDPRASEAGILEILRTAAGRAGLRGANTILIAADLAGTTHSGRRYPPFVSPPF
                     LRPEVESAGAERQQVLCYDTNPEDIRTALAAWTWVDGSLGDTYPLQFLSWDPQVEELI
                     ATTAAEFDVADSVRTRPPTKLLSQLYRRAAAVLSVRLVAWGQPFRWALASGVPVVGFE
                     SPSANSILGDAGYLVPDGDTRALGAACLSLLVQDELAESLRVRGRRRAAAYRDRPPVE
                     LIAELADRSGSLVGRRAPS"
BASE COUNT          658 a         1241 c         1225 g          648 t
ORIGIN      
        1 ccgggctggt cggtgccgag ctgggggagg cgatcatcgg cgacctacag gaactccgcc
       61 cgacagtcga ggctcactgg ggcaagggtg ggtcatggtt gtggctggct ctggaagtcg
      121 tgcgtggcgc ggcagtgcta accgctcagc ggctgagtcc ggcgagcggg cgtagaaagg
      181 agaatcgatc gatggacaac tggaaagaga ctgtcaggcg ggctgcggcc gcaatcgggc
      241 tgatcgcctg ccttccagcg gcggtgctgg tagtcggagg gctgctgcag agcgactgga
      301 gcgctccggc ggttcggtcg gcactcgagc ggaccctgtt caatccggac ctggtcgggt
      361 tccgggtgct gatccatccg gcgacaattc tgtccgggct gctcctggcg gccggactga
      421 acctcatccc gctattgcgc atcggactgg aacgccggcc gggaactcta gtcggcaccc
      481 tggcgcttcg gatccgctcc gcccacctgg gggttgcggt tgtcgccctc ggcctgttgg
      541 gcgttatcct tggctatgga tttaccgaaa acttcagggt cgtgccgacc catgtgagcg
      601 tccaggtgtc aggttggcag atcgagcggg atctcctgat ggcggtttgc gaggaggccc
      661 agtgtgggca gcgatgaccc gctgatcctg gccatcacga gtgattactt cctgatcccc
      721 cgcttggaag ataccgcccg cgccctcggt ttccggctgg aggtcgttca gaccgcggcg
      781 gacctcggag cggaggggcc tcctattccc agagccattc cgctcaccga gcccttggaa
      841 ggccccgatg cagcctttgt gcggagccta gtcgaccggc gccccgcctt gctgctggtg
      901 gataccgctc acgatggggt gccttgggag cactggatcc agaccctgaa gaccggctcg
      961 gcgacccgcc gaatcccggt ggtcgccttc ggtccgcacg tcgaggccga ggtactgcag
     1021 cgggcgatcc gggccggcgc cgaggaggcg gtaagcctcg ggcaacttca agccagcttg
     1081 ccccagatca tcgagcgccg ggcccggcga atcgatctgg ccgccttgaa agaggcctgc
     1141 gaccaaccgc tctctacctt gggtgcccat gggctcgggc tattgaactc cggagagtac
     1201 ttcgaagcgc atgaagtctt ggaaaaagcg tggatggaag ctccggagtg gcacggctac
     1261 ctctaccggt ctctgctcca ggtgtccgta gcgtatttgc aggtccagcg ggcgaactac
     1321 cccggggcgg tcaaaatgct gctgcggctg aaacagtggt tagagccgct gccggatcgc
     1381 tgcagcggga ttgatatcgc cgcgctaaaa cggaacatgg ccgagttctc cgcggcggtc
     1441 gaggccgccg gggtagatgg aatcgcgacc ctcgatccgg ggctgctgcg gccggtctta
     1501 agtcaggaat agcgggcgtc ttcggtcttg agctcgcccc gccgcctcca gcggaagcgg
     1561 tgggcgaagc ggttcggctt gcgctccagc acatcggcaa tgatcgggcc gagcatgggt
     1621 gcgaatttga agccatggcc gctgcctccg gccgcgacca gcagaccctc ccgatctgga
     1681 tcgaaatcga tccagaagtc cccatcccag gtgtcgcagt acatgcacag ccggttgctg
     1741 atcatcggcg cctcggctag cgccggcagc gaacggcgga agaaagcccg gaacatctcc
     1801 tcatacccgg gatctacctg ccgcggttcg gcggcggctt ggcgccgccc gggcccatgg
     1861 ttggcgacct tcacggtccc atcgtcgagc gccgggaatc cgtaccatcc ggtgcctgcg
     1921 atatcggcgg accacaccgc gaatttcggg ggctggaatg actcgatgtc ttctacccgg
     1981 aagtggaaaa ccggctggcc ggtcgaccac atgaggtctt ctagctgtgg cagcagcgcg
     2041 gggctccagg ccccggctgc gagcacgaca tactccgctt cgaattcccg cccatccgcc
     2101 atccgcaccc catgcactcg cttcccttgc tcgatcaggg tgcgcatctc cgcgcttgcg
     2161 tagaccttga ccccaagttt ctttccatct gcgatgaggc gggccaccac ctcgccgctc
     2221 tcggcccaac cggcccgcgg gttgaaatag gcctccgggt ggtcggccgg gtcccaggcg
     2281 ggaaagcgct ccttgatctg ggcggccgat aaccgttcgg ggtgatggcc gcgggcgcgc
     2341 agcagttggt agctgtcgtg ttcgaagcca cccggcgcca tgggcccacc ggccagcaag
     2401 agcattccgg tctggtggaa gagcggctcc ccccacccct cgttccacgc gtcccacccc
     2461 tggaacgcgg cctccatcaa ttccatatag aactcgtccg caccgtaatc catgcgcagg
     2521 actttgctga tgtcggtcga ggcggccagc acgtgcggca ccggcccagg atcgagcagg
     2581 gtgacgatgt ggccgcgttt gcgcaattca agcgcggccg aagctccgaa aacaccggcc
     2641 ccgacgatga caaccgagcc gtccgcctgt ccggccattc tcagctcaat ccaccagatc
     2701 tgacgatttc aagaaggtgc acgccttcct accagggaac ccgaccggtc agccagctcg
     2761 gcgatcaact ccaccggtgg gcgatcccgg taggccgcgg cccggcggcg acccctgacc
     2821 cgcagcgatt cggccaattc atcctggacc agcagcgaca gacaagccgc cccaagcgcc
     2881 cgagtatctc catccggtac cagatatccg gcgtcgccca gaatcgaatt cgccgacggc
     2941 gattcaaacc ccaccacggg taccccgcta gccagcgccc agcggaaggg ctgaccccag
     3001 gctactaaac gcaccgacag aactgcggcc gccctacgat acagttgaga aagcagcttg
     3061 gtcggtggcc gggtcctgac cgaatcggcg acatcgaact cggcggctgt cgttgcgatc
     3121 agctcctcga cctgagggtc ccagctcagg aattggaggg gataagtgtc ccctagcgag
     3181 ccgtccaccc aggtccaggc tgccagcgcc gtgcgaatgt cttccgggtt cgtatcgtag
     3241 cacaggacct gctgccgctc cgcgccagct gactcaactt cgggccttaa gaagggcggt
     3301 gaaacaaacg gagggtagcg ccgtccggag tgcgtcgttc cggccagatc ggccgcaatc
     3361 aggatggtgt tcgcgccgcg gaggcctgcc cggcccgccg ctgtcctgag gatctccaga
     3421 atcccggctt cggaagcccg cgggtcgatc ggagggacga ccgcgatcgg catccgggcc
     3481 ttcagcggaa tcccatccga gaaggtcaac aagagttccg cacccgcctg cgccacggcc
     3541 cgcggaagag tgatctgatc gtagcggagc cgctcccagc cacgccggat gggccctact
     3601 ggccaggaac gaacccccgg cggaagctcg ggcagctccc cgtccggatg caccagggag
     3661 aatttcgtct ccggacccga gaggccattg atccattcga ggagatgctg ggccgccgct
     3721 gaggtcgggt gctcgacgag cggtcggcca tcgactgcaa tgtgaatcag at
//
